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Table 4 Details of reaction mixtures used for HFE genotyping

Primer Sense/ Primer sequence HFE No. antisense nucleotide annealing position F1 S AAGCAGCCAATGGATGCCAAG 948–968 F2M S GGGAAGAGCAGAGATATACGTA 1045–1066 F3 S AGCTGTTCGTGTTCTATGATC 388–408 F4 S AGCTGTTCGTGTTCTATGATG 388–408 F5 S TTCGTGTTCTATGATCATGAGA 393–414 F6 S TTCGTGTTCTATGATCATGAGT 393–414 F7 S TTCGTGTTCTATGATGATGAGA 393–414 F8 S TTCGTGTTCTATGATGATGAGT 393–414 R1 AS CCACTGATGACTACTCCAATGACTA 1135–1156 R2W AS CCTGGGTGCTCCACCTGGC 1066–1085 R3 AS CTGTGGTTGTGATTTTCCATAA 535–566

Specificity of the primers is determined by the underlined 3(cid:6) nucle- otide. Primers F1, R1 and R3 are consensus non-allele specific pri- mers. References in the text. Nucleotides are numbered according to Feder et al3 (Genbank accession number U60319).

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