An integrated system for high throughput Ta

an based SNP genotyping. Bioinfor- matics

,

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Harman, D.,

Free radical theory of aging. Triangle

,

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Herskind, A.M., M

ue, M., Holm, N.V., Sorensen, T.I., Harvald, B., Vaupel, J.W.,

The heritability of human longevity: a population-based study of

Danish twin pairs born

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Hum. Genet.

,

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Hjelmborg, J.Vb., Iachine,I., Skytthe, A., Vaupel, J.W., M

ue, M.,Koskenvuo, M.,Kaprio, J., Pedersen, N.L., Christensen, K.,

Genetic influence on human lifespan and longevity. Hum. Genet.

,

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Holzenberger, M.,Dupont, J.,Ducos,B., Leneuve,P.,Geloen,A., Even,P.C.,Cervera, P.,Le,

In conclusion we here present new candidate variations for human longevity located in the DNA damage signaling and DNA re- pair, the GH/IGF-1/INS signaling and pro/antioxidant pathways. We find that 4 of the 17 identified SNP associations show significant and similar direction of effect when using the two different study ap- proaches applied to the Danish discovery cohorts, while 2 SNP associ- ations showed similar tendencies. However, none of the 17 SNP associations displayed replication in the independent samples from Germany and the Netherlands, although for 2 SNP associations some support was noticed.

Conflicts of interest

The authors have no conflicts to declare.

Acknowledgments

This study was supported by the Max-Planck Institute for Demo- graphic Research (Rostock, Germany), the INTERREG 4 A programme Syddanmark-Schleswig-K.E.R.N (by EU funds from the European Re- gional Development Fund), the National Institute on Aging (P01 AG08761), a Dutch grant from the Netherlands Consortium for Healthy Aging (Grant 050-060-810) in the framework of the Nether- lands Genomics Initiative/Netherlands Organization for Scientific Research (NWO), the European Union's Seventh Framework Pro- gramme (FP7/2007-2011) under grant agreement n° 259679, the Novo Nordisk Foundation, the Aase and Ejnar Danielsen Foundation, the Augustinus Foundation, the Brødrene Hartmann Foundation, the King Christian the 10th Foundation and the Einer Willumsens Mindelegat Foundation. The Danish Aging Research Center is sup- ported by a grant from the VELUX Foundation. Susanne Knudsen, Steen Gregersen, Ulla Munk, Shuxia Li, Anne Mette Hedegaard Nielsen, Marlene Graff Sørensen and Lene Elnegaard are thanked for excellent technical work.

Lisa, R., Spazzafumo, L., Amadio, L., Giunta, S., Stecconi, R., Morresi, R., Viticchi, Mattace, R., De, B.G., Baggio, G.,

Do men and women follow different tories to reach extreme longevity? Italian Multicenter Study on (IMUSCE). Aging (Milano)

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Giannakou, M.E., Goss, M., Junger, M.A., Hafen, E., Leevers, S.J., Partridge, L., Long-lived Drosophila with overexpressed

OXO in adult fat body.

,

Gueorguiev, M., Lecoeur, C., Meyre, D., Benzinou, M., Mein, C.A., Hinney, A., Vatin, Weill, J., Heude, B., Hebebrand, J., Grossman, A.B., Korbonits, M., Froguel, P., Associationstudies onghrelinandghrelin receptorgenepolymorphisms sity. Obesity

,

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(Silver.Spring). Hampe, J., Wollstein, A., Lu, T., Frevel, H.J., Will, M., Manaster, C., Schreiber, S.,

integrated system for high throughput Ta

an based SNP genotyping. matics

,

–

Harman, D.,

Free radical theory of aging. Triangle

,

–

Herskind, A.M., M

ue, M., Holm, N.V., Sorensen, T.I., Harvald, B., Vaupel, J.W., The heritability of human longevity: a population-based study of

twin pairs born

–

Hum. Genet.

,

–

Hjelmborg, J.Vb., Iachine,I., Skytthe, A., Vaupel, J.W., M

ue, M.,Koskenvuo, J., Pedersen, N.L., Christensen, K.,

Genetic influence on human lifespan longevity. Hum. Genet.

,

–

Holzenberger, M.,Dupont, J.,Ducos,B., Leneuve,P.,Geloen,A., Even,P.C.,Cervera, B.Y.,

IGF-

receptor regulates lifespan and resistance to oxidative stress mice. Nature

,

–

Hubacek, J.A., Bohuslavova, R., Skodova, Z., Adamkova, V.,

Variants within ghrelin gene-association with HDL-cholesterol, but not with body mass Folia Biol. (Praha)

,

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Jacobsen, R., Martinussen, T., Christiansen, L., Jeune, B., ndersen-Ranberg, K., J.W., Christensen, K.,

Increased effect of the Ap

gene on survival at vanced age in healthy and long-lived Danes: two nationwide cohort Aging Cell

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Kawano, T., Ito, Y., Ishiguro, M., Takuwa, K., Nakajima, T., Kimura, Y.,

cloning and characterization of a new insulin/IGF-like peptide of the Caenorhabditis elegans. Biochem. Biophys. Res. Commun.

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Appendix A. Supplementary data

Supplementary data to this article can be found online at doi:10.

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