BEEBERERPEESRREEREEPPERERE RSPR REESE EP ERE RE EE0 \oGrpllGrp9Grp5Grp4Grp3Grp2Grpl

Grp7J (16065g)

“* 45" criteria. * . This \ s WAS | ee NE IIINIDs performed t reneebyt y using t   ot — i> cluster ¢ r of | EEE IELTSf programmes i sint 1 the I /9#1(cid:24)/ Jp Meee> package ( OL IN> (Version - 4(cid:28)%(cid:5)) ee OD; Felsten- stein, | +;;4>(cid:28) oe t. The t oe: restriction 1 ay Meee1 matrix |  ume Mewas | EMORY; bootstrapped | +((( ) times, 1 , Maximum | ae Pee ee| parsimony t ~A\rh?./ trees t     > Wagner method \ eae *1 were t > then § 1 generated f 1 from t 1 this ¢ s data < 1 and f terres **1 finally, < , ac 1 consensus t s tree \ > was ¢ ne PO ODs obtained. I ae ere. Haplotype « oN> codes ( come eo”s (hap | +A *3> ) correspond t ltot “ eNemes» those vu > used i lint 1 the t > text. 7 . The e > eleven ¢ eee I eM1 groups ¢ s of r  f related | 1 haplotypes f s formed ( <@(cid:9)(cid:15) +A++> ) are i ohne: ae> indicated ONSont Vy ee1 the | eNO eee aes> branches « .of ¢ f each ¢ 1 Grp ¢ ) and t 1 the J & we RN EN OEE| haplogroup ( <@(cid:9)(cid:15) ,> ) is I ENOL EEEs highlighted | | by t the s a INA NO> square | ere» bracket.

)*+* . Ross ¢ yet ¢ tal. / , / Experimental ( | Gerontology - /0 123345 4404644(cid:30)7

sdnoid oSe yi0q 10j ([[—] dD) sdnosSojdey oy Jo sorouonbary oy, uosteduios yore Joy onyea-d jOeXe S,JOYst-] Oy) SMOYS 97Q¥) BY) JO UNOSTe ee i dana aps Ca nacepd aan cad Cantden am cuaane arian a  (dey) odXjojdeyy — dnosojdey (%) 671 =U IL (YC6=UTRWAy SE=UIRW (%)OOl=4[IOL eG =uoeWoy [p= ude  %444(cid:28)(>3<::A>*<*+4%*(cid:30)**(cid:30):’(cid:30)%’(cid:30)4++(cid:15)(cid:9)@ ((+3(cid:28)(>*<%’4>*<**A:4(cid:30)%4(cid:30);’(cid:30)*’(cid:30)’’(cid:30)*+’(cid:15)(cid:9)@ 3+:’(cid:28)(>(*<’%%4,+>%*<%**’+’’*(cid:30)+’(cid:30)3+(cid:30)’+(cid:30)’4(cid:15)(cid:9)@ (*3+(cid:28)(>%<,%’>’<’’A,4(cid:30)*4(cid:30)’4(cid:30)(+(cid:30);*(cid:15)(cid:9)@ (3*((cid:28)(>,<;,’>%+<%+(+%(’(cid:30);+(cid:30)*(cid:30)4%(cid:15)(cid:9)@ ;*;%(cid:28)(>’<’A’>+<+A+(4(cid:30)3’3(cid:15)(cid:9)@ ;334(cid:28)(>’+<%+++*>*+<*+:3+*(cid:30)34(cid:30)+4(cid:30),’(cid:30)4’(cid:30):+(cid:30)3(cid:30)%,(cid:15)(cid:9)@ 4(4((cid:28)(>%<3*’AAA3*(cid:30)4*(cid:30)(*(cid:30)44(cid:30),+:(cid:15)(cid:9)@ 34(*(cid:28)(>:<(+3*>3<3%+;4(cid:30):(cid:30),;(cid:15)(cid:9)@ ;+:((cid:28)(AAA>4<4’+%+(cid:30)++(+(cid:15)(cid:9)@ ,(+(cid:28)(>’+<%+*++>3<344+++(cid:15)(cid:9)@

/*?* (cid:18)(cid:4)<4(cid:30)7 and | (cid:18)(cid:4)(cid:29)3<< | polymorphisms

The same control and aged cohort of samples were examined for the two mtDNA nucleotide | eR IED DED IIL OESpolymorphisms, onat | positions (cid:17)(cid:3)%+,: and (cid:17)(cid:3);(%%(cid:30) associated with longevity. In| the | eeCase | of | sd oe* the 1 (cid:17)(cid:3)%+,:(cid:14)  _ polymorphism, ;  all: ’’; —— “Sao| individuals : from | the |  Trish | 8 Om ee| population tested 1 | negative | for | ‘this | eh pee ee; polymorphism | aan eeey SS| (Mito - 4 | probe). . AL ’’; ) individuals | meee TEtested | eee| positive for t r the 1 (cid:17)(cid:3)%+,: SE EEE Ee,’ sequence | ed OEEusing 1 ‘oer the | RNA EE MANE> Mito + wm“ Peeve| specific | Me pe eae: probe. ] Inc 1 order t to ) verify t ee |' these 1 aresults < 5a cohort. of 4(  wee OD(Singapore | “hinese) “epeeDNA asamples were | examined yy “oe”under the | exact same ( - conditions neenee XOsand 1 Ane’| the 1 (cid:17)(cid:3)%+,:(cid:14) ee ENN PN ees. polymorphism mp| Was ¢ ; observed in: | SIX | . samples | <’(=> ) and the 1 (cid:17)(cid:3)%+,: ' polymorphism | in! ’* pee tee Esamples | <:(=>) no | . heteroplasmy » | was % nee pee vee; apparent. ' OF_ These samples ' a; were t re’ws*®> then « ONE OEE| employed las | eeepositive « Powe NM> and 1 ANM IM Fe ae| negative EN ONE EO> controls % roesand ¢ | all t | the ¢ > aged < | and « 1 control ens weialsamples NN END EE ALISITre-examined pew I™and the epee &previous NE I ESresults > NOEconfirmed, indicating aan ‘orabsence of the (cid:17)(cid:3)%+,:(cid:14) polymorphism in the Irish population. The I LENE IIISpolymorphic GCC ooomtDNA nucleotide DRE EEE” EEassociated ree Jaewith an increased eee PMEfrequency in French oe pen J ees eescentenarians <(cid:17)(cid:3);(%%(cid:14)> was also observed | Pat | -an increased | ~eee_ frequency iwithin | the Irish | aged | EERE ERENT NO/community | <+’B+’;(cid:30) ;= O * male/8 female) - pin NE NE| comparison | to | the « control | cohort | © “S*Of | samples |   <%B+(((cid:30) %= O 4 male/2 female). . PEE FORE_ Although t 7 eee bethese 1 results 1 reflect t t those « - observed j int eel\ the | epee wees: French : oe| study 1  | statistical NEA N Fanalysis \ s WAS | ne; performed | | using | ; Fisher’ ¢ s exact | t probability t test 1 [no § ENE ONIN ON) significance ' > Was ( ; observed, EE POEP-value ( ((cid:28)+3*(cid:28) .Nos CELI LL ES) significant SNE NESSt association | va" Fe1 Was ¢ ——e—e—er—eee*s observed | J «nee| when 1 me SES: male < >and { | female NE NOD» frequencies were ¢ - analysed | NN| separately, | however | the j increase | in | \ the 1 (cid:17)(cid:3);(%%(cid:14) . polymorphism | 7“| Was | » More marked | “DSin | io“—_the | pee eesfemales | <4 oaVS | ;=> } compared | | to | the 1 males | <, VS +(=>(cid:28)

 , Discussion