Results:
The ApoE 4 allele was significantly associated with AD (p < 0.00001; OR 3.74; 95% CI: 2.57-5.45). The distribution of the rs3851179 genotypes and allele frequencies in the centenarian group shows a statistically significant difference compared to the group of healthy subjects: OR 1.92 for the AA genotype (95% CI:1.00-3.67, p = 0.033) and 6, 7]. After stratification by gender, the statistically significant value of the distribution of the A allele was confirmed among centenarian females: OR was 1.46 (95% CI: 1.00-2.14, p = 0.042), while no differences in genotype distributions of rs3851179 were observed in Hardy-Weinberg equilibrium in all the studied groups. No significant association between mean age-at-onset of LOAD or mean age of the centenarians and distribution, odds ratios (ORs) with corresponding 95% confidence intervals (CIs), p-value of PICALM rs3851179 polymorphism in the studied groups. Total genotypes and alleles were GG GA AA G A Control, n (%) 359 145 (40.4) 153 (42.6) 61 (16.9) 443 (61.7) 275 (38.3) Females 201 78 (38.8) 85 (42.3) 38 (18.9) 241 (59.9) 161 (40) Males 158 67 (42.4) 68 (43) 23 (14.5) 202 (64) 114 (36) Centenarians n (%) 105 31 (29.5) 49 (46.7) 25 (23.8) 111 (52.8) 99 (47.2). OR (95% CI), p-value:1*1.50(0.88-2.56),0.11;1.92(1.00-3.67),0.033;1*1.44(1.04-1.98),0.021 for GG, GA, and AA genotypes respectively.Females n (%)81;21(25.9);40(49.4);20(24.7);82(50.6);80(49.4). OR (95% CI), p-value: 1*1.75(0.91-3.37),0.07;1.95(0.89-4.3),0.067;1*1.46(1.00-2.14),0.042 for GG, GA, and AA genotypes respectively.Males n (%) 38 (18.9);24;10(41.6);9(37.6);5(23.8);29(60.4);19(39.6). OR (95% CI), p-value: 1*0.89(0.31-2.55),0.80;1.3(0.59-2.9),0.52;1*1.19(0.60-2.26),0.63 for GG, GA, and AA genotypes respectively.LOAD n (%) 349;140 (40.1);154 (44.1);55 (15.7);434 (62.1);264 (37.8). OR (95% CI), p-value: 1*1.04(0.75-1.46),0 .80; 0 .93 (0 .59-1 .47) , 0 .75 ; 1*0 .98(0 .79-1 .22) , 0 .85 for GG, GA, and AA genotypes respectively.Females n (%)229;92 (40 .2) ;101 (44 .1) ;36 (15 .7) ;285 (62 .2) ;173 (37 .8). OR (95% CI), p-value: 1*1 .01(0 .65-1 .56),0 .97 ; 0 .80(0 .45-1 .44),0 .43 ; 1*0 .91(0 .68-1 .21),0 .49 for GG, GA, and AA genotypes respectively.Males n (%)120;49 (40.8);54 (45);17 (14.2);152 (63.3);88 (36.7). I. Piaceri et al. / Implication of a Genetic Variant at PICALM in Alzheimer's Disease Patients and Centenarians were shown in LOAD (Table 2). After stratification, no differences were observed among all studied subjects with the presence/absence of APOE4 allele (data not shown). This is a negative finding for the limited number of men (24 out of 105) in the centenarians. We have analyzed PICALM on centenarians for the first time and provide evidence of its implication in the genetic architecture of longevity. 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