Table 3

Haplotype frequency distributions of IL-1 gene cluster in 688 young subjects, 309 elderly subjects and 134 centenarian subjects from Northern and Central Italy

19(cid:1)64 years (n(cid:5)1376, %9S.D.) 65(cid:1)99 years (n(cid:5)618, %9S.D.) 100 years (n(cid:5)268, %9S.D.) / / / / / / / / 1CC 454 (0.3390.01) 204 (0.3390.02) 94 (0.3590.03) / / / 1CT 206 (0.1590.01) 80 (0.1390.02) 43 (0.1690.02) / / / 1TC 261 (0.1990.01) 124 (0.2090.02) 51 (0.1990.02) / / / 1TT 55 (0.0490.01) 31 (0.0590.01) 8 (0.0390.01) / / / 2CC 124 (0.0990.01) 37 (0.0690.01) 21 (0.0890.02) / / / 2CT 165 (0.1290.01) 80 (0.1390.01) 29 (0.1190.02) / / / 2TC 28 (0.0290.01) 19 (0.0390.01) 5 (0.0290.01) / / / 2TT 55 (0.0490.01) 19 (0.0390.01) 8 (0.0390.01) / / /

Estimation of maximum-likelihood multi-locus haplotype frequencies was calculated using an EM (expectation maximisation) algorithm implemented in the Arlequin package. x2-tests for comparison of haplotypic distribution was performed by use of Monte-Carlo algorithms implemented in SPSS. x2(cid:5)13.366; d.f.(cid:5)14; P(cid:5)0.507. / / /

    1500510004(qwy/8d)ey1-T1 Age

Acknowledgements

This work was financed by Ministry of Health Projects ‘‘Analisi delle risposte immunitarie dopo som- ministrazione di vettore plasmidico codificante per VEGF umano e studio dei polimorfismi delle citochine pro-infiammatorie nell’infiammazione cronica eta`-corre- lata’’ to C.F. and ‘‘Immunological parameters age- related’’ and ‘‘Pharmacogenomics of Alzheimer’s dis- ease’’ to C.C. The collaboration between the Palermo group and the Istituto Nazionale di Riposo e Cura per Anziani was enhanced by a cooperation contract (Long- evity and elderly disability biological markers) and by the EU thematic network program ImAginE (QLK6- CT-1999-02031). L.C. is a post-graduate student of Ph.D. course in pathobiology directed by Prof. C.C.

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