) The SIRT

polymor- phism rs

and risk of Alzheimer's disease in two Caucasian case–control cohorts. Alzheimers Dement

(

):

–

Polito L, Kehoe PG, Forloni G, Albani D (

) The molecular genetics of sirtuins: association with human longevity and age-related diseases. Int J Mol Epidemiol Genet

:

–

Rose G, Dato S, Altomare K, Bellizzi D, Garasto S, Greco V, Passarino G, Feraco E, Mari V, Barbi C, Bonafe M, Franceschi C, Tan Q, Boiko S, Yashin AI, De Benedictis G (

) Variability of the SIRT

gene, human silent infor- mation regulator Sir

homologue, and survivorship in the elderly. Exp Gerontol

:

–

Seripa D, Pilotto A, Panza F, Matera MG, Pilotto A (

) Pharmacogenetics of cytochrome P

(CYP) in the elder- ly. Ageing Res Rev

:

–

We acknowledge that this assessment suffers from limitations; first of all, the lack of independent tissue source to confirm a widespread and not tissue-specific effect. Moreover, we are not able to rule out the possi- bility that other genetic or epigenetic modulations oc- curring in PBMC might have affected the transcription SIRT3 rate of the gene. Finally, the exact VNTR se- quence linked to rs11555236 (T) allele should be fur- ther investigated.

In summary, this study points to an involvement of SIRT3 variability in human longevity, with a particular SIRT3 emphasis on the role of the rs11555236 poly- morphic site as a genetic and functional marker.

Acknowledgments This work was supported by “Fondazione Italo Monzino,” Milan, Italy and by grants from “Interdisciplin- ary Geriatric Research Foundation (FORGEI)” and from “Fondazione Veneto Banca.”

References

Albani D, Batelli S, Polito L, Prato F, Pesaresi M, Gajo GB, De Angeli S, Zanardo A, Galimberti D, Scarpini E, Gallucci M, Forloni G (

) Interleukin-

plasma level increases with age in an Italian elderly population ("The Treviso Longeva"- Trelong-study) with a sex-specific contribution of rs

polymorphism. Age Dordr

:

–

Albani D, Mazzuco S, Polito L, Batelli S, Biella G, Ongaro F, Gustafson DR, Antuono P, Gajo G, Durante E, Caberlotto L, Zanardo A, Siculi M, Gallucci M, Forloni G (

) Insulin- like growth factor

receptor polymorphism rs

and circulating interleukin-

level affect male longevity in a population-based prospective study (Treviso Longeva- TRELONG). Aging Male

:

–

Albani D, Polito L, Forloni G (

) Sirtuins as novel targets for Alzheimer's disease and other neurodegenerative disorders: experimental andgenetic evidence.J Alzheimer

is

:

–

Anselmi CV, Malovini A, Roncarati R, Novelli V, Villa F, Condorelli G, Bellazzi R, Puca AA (

) Association of the FOXO

A locus with extreme longevity in a southern Italian centenarian study. Rejuvenation Res

:

–

Bause AS, Haigis MC (

) SIRT

regulation of mitochondrial oxidative stress. Exp Gerontol. doi:

/j.exger.

Bellizzi D, Rose G, Cavalcante P, Covello G, Dato S, De Rango F, Greco V, Maggiolini M, Feraco E, Mari V, Franceschi C, Passarin

, D

enedictis G (

)A nove

NTRenhancer within the SIRT

gene, a human homologue of SIR

, is associated with survival at oldest ages. Genomics

:

–

Benigni A, Corna D,Zoj

, Sonzogni A, Latini R, Salio M, Conti S, Rottoli D, Longaretti L, Cassis P, Morigi M, Coffman TM, Remuzzi G (

) Disruption of the An

I type

receptor promotes longevity in mice. J Clin Invest

:

–

Cederholm T, Persson M, Andersson P, Stenvinkel P, Nordfors L, Madden J, Vedin I, Wretlind B, Grimble RF, Palmblad J (

) Polymorphisms in cytokine genes influence long-

Yu W, Dittenhafer-Reed KE, Denu JM (2012) SIRT3 protein deacetylates isocitrate dehydrogenase 2 (IDH2) and regulates mitochondrial redox status. J Biol Chem 287:14078–14086