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longevity  the aTrish eepopulation

Blood‘ ELIE IE IEEE| Transfusing | eee A: Building, | OE_ Belfast | t NII | BT9 | )7TS, | !

Received  % July 4 ’((() ; received i lint 1 revised | form < * | January < ’((+) ; accepted 7 , | February ‘



The 1 >mtDNA § . genome |   s been i 1 implicated las f s playing <  1 pivotal 1 | role i > in ¢ 1 determining t : the | > longevity y and   s of t f the I me oouhNN™ee”> human | 1 lifespan. / .Al / (cid:29)0(cid:29)-1/ BPO EEE SP> methodology \ eeey was U s used t ltoi ) identify f REEDED LON IDESy polymorphic 1 Seorress ooees> restriction enzyme s - sites | ; within ; la ’3*4 } bp 1 ) region « \ of t F the 1 > mtDNA ; oy Tew”. genome % and ¢  lat 1 table « eee- of § J reger genetic | oe pene aoe- haplotypes { s for ¢ Pa OO ONhealthy | aged | and ;  | younger “wr Soocontrol « _ cohort | - of 7 anes oN* patients ° | Was | ; constructed. | — wn BeForty-six « ee OPE ITdifferent 1 reeem se-mtDNA ONDE OEhaplotypes <   ++ OFS| groups ¢ ‘ousoft f related I i haplotypes \ ‘renes were i > identified | across t s the t > two i wee ye) age § > groups | s but s t statistical Tepesanalysis f eeorns failed t Ito s nn — ieee |) show < “pre M*y any s ENN ETS/ significant < eres’ Pee Nst associations. "  . The I > European J & EO NEE RANJ haplogroup, | =~ ore ee, previously r / reported t Ce) be< COND OED OLNassociated \  1 with | EEN OED OTS1 longevity, \ , Was I s not { t found « lat < tani 1 increased f  eM ee AOSD| frequency \ “eeo™; within t 1 the J   reemee ®1 aged | co =———_——e——_—erTen| population (cid:1)’   ((cid:1)43(cid:3)(cid:1) NE EIHowever, | Srthe | “yo Trews ae- haplotypes | ; comprising | the . & eShaplogroup « 2 o*oV_—_—) could | | be nae ARE OEE Be PMdifferentiated | pee| into | ) two Me Meedistinct | ow sper- branches | by | the | ee EOP EI E- presence | meOF | ins ieneabsence eeof | the | / two | eee sr eee} polymorphic | restriction | | sites, +34:;(cid:21) and +3((((cid:21)(cid:28) . The | pS» branch « | of | ~ eeehaplotypes ¢ : defined | | by +34:;(cid:21) AM RMAsisplayed  atbas tpt. jeeignificant  | increased { i AMMEN| frequency i yin the < wee> aged s 1 samples ( <:=> oe pees) compared t Ito t ) the ¢ ee> controls ( <+=>(cid:30) ’  ((cid:1)(+%(cid:1) ees. Inversely, t , the | > branch « 1 of | fe Ne PORESf haplotypes eee eee ze ee eee EN I NIN Nt NI IIII IDI SD NID Fig ENE NINE IN LE LIND IA IDI EI OLED I EOdefined by 16000g displayed a significant decreased frequency in the aged samples (4%) compared to the controls ( <+4=>(cid:30) ’  ((cid:1)(++(cid:1) eee. The ee ES NE OO- polymorphism | <(cid:17)(cid:3)%+,:(cid:14)> “J 0h NN ee) associated \  | with | eApeNee NES): longevity i pwint CADETS1 the | > Japanese was | not f PEM INSt found i Fo *lint 1 the | > Irish ] ae RAEN pee ye”| population, \ Aa ees, while t meee X> the | © Laan EM AP aus- polymorphism ( <(cid:17)(cid:3);(%%(cid:14)> Bh ENED ALT ®) associated |  | with s “Nee eee1 successful ageing i   1 the F pe AREER LEE” PS eee> French centenarians \ s was f s found 2 lat z hE PM FLIESt an increased f ee PE NEEDS NAD AD1 frequency int we As ©1 the I   1 aged population ( <;=> eowene nncompared t ltot ) the 3 > younger ¢ r control § | group ( <%=>(cid:30) , but f t failed t ltor “eee”) reach < ral hae Jaelevel ¢ | of s ae EE SNf statistical s eM PMP MA eenen %| significance, | ’  ((cid:1)+3*(cid:1)  ’((+ me Ieee| Elsevier S r Science | oT”> Inc. : Alt Ws se| rights 1 s reserved.

Keywords: } : Mitochondrial | | DNA; I ; Longevity; J & | Haplogroup; | ; Polymorphism

"  Corresponding < y author. / . Address: I : Northern I 1 Ireland I 1 Regional | | Histocompatibility < y and I 1 Immunogenetics | tory, E — mop, Belfast ¢ eeCity I me Ney Hospital,  , Blood 1  1 Transfusing I » Building, I  renee”, Belfast tNIE $.; ,.?(cid:30) DE(cid:28) ro. Tel.: - (cid:1)**0+’4’0’304:0:4) (cid:1)**0+’4’0’304:0:((cid:28)

E-mail « | address: 1 - martin.curran @bll.n-i.nhs.uk ( : (M.D. ¢ Curran).

(%4+0%%3%B(+BC 0 see | > front 1 t matter  ’((+ | Elsevier r Science | > Inc. 4 . All | rights 1 s reserved. PII: ?(%4+0%%3%<(+>(((;*0:

 _ Introduction