According to NFkB gene polymorphism, our study did not find any difference in genotypes/allele frequency between groups. Some studies have suggested that NFkB gene polymorphism is involved in aging.

The current study is a candidate-gene association study that compared nonagenarians and centenarians with younger individuals with no possible prediction of their future longevity. Also, the number of participants is relatively small, but we should keep in mind that nonagenarians and centenarians are usually not very enthusiastic to visit hospitals unless it is an urgent health problem. Finally, our study did not have ACE and CETP levels measured in our cohorts.variety of polymorphisms and their relation to human longevity was conducted. To avoid misclassifying heterozygotes as homozygotes, a second PCR was performed. The study focused on individuals from Athens, who were reasonably homogenous in terms of geographical and ethnic origin, with the majority being nonagenarians and centenarians. The study found that there were differences in the frequency of alleles in the oldest two decades compared to middle-aged patients. Understanding aging at a molecular level could provide an option to slow this process and extend lifespan. The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. The authors received no financial support for the research, authorship, and/or publication of this article.

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