The F62L polymorphism is a rare polymorphism or a sporadic mutation since it was not observed in 1390 additional alleles that were examined. Another mutation V80L seemed to be limited to African Americans, and in the sample of 600 African American alleles examined no relationship to aging was apparent. One polymorphism, the 1648 A0G (I550V), which initial studies suggested were in excess in the older population, was considered more extensively for its association with lifespan and body weight. An examination of 1338 female and 684 male white subjects demonstrated that the 1648 A0G (I550V) mutation was not associated with an increase in lifespan and not related to lower body weight, suggesting an effect on caloric restriction.

Because of the proposed catabolic effects of Indy mutations, we calculated the average heights and weights of white subjects with different genotypes. The results did not demonstrate an association of genotype with height and weight.

We have identified three mutations in the human Indy gene that result in an amino acid change. None of the mutations were observed to be associated with longevity or body weight. Our study involved a small number of elderly subjects (age 90-99), and thus only the presence of a high-frequency longevity-promoting mutation would have been observed. The examination of a larger elderly population would be necessary to identify less common longevity-promoting haplotypes or polymorphisms in the Indy gene. Furthermore, since our study involved only unrelated subjects in a normal population, it remains possible that mutations in the Indy gene might play a role in the increased longevity seen in familial centenarians.

The authors would like to acknowledge Dr Jill Waalen and Carol Halloran for their expert assistance. This is manuscript number 14417-MEM from The Scripps Research Institute. Supported by grant DK53505-04 and funds from the Stein Fund.I'm sorry, but there is no text enclosed in ``` ``` for me to proofread and edit. Could you please provide me with the text you want me to process?