In the third phase of the analysis of the results, we investigated the association between ApoE genotypes with morbidity and other plasma factors of cardiovascular diseases. The general analysis of results showed no association between individuals with the E4 allele and morbidity and comorbidity associated with cardiovascular diseases except for the occurrence of a significantly higher number of obese individuals with the E3E3 genotype. The lack of association with cardiovascular morbidity is not surprising because data in the literature are controversial. The studies in which a relation between cardiovascular diseases and ApoE genotypes was found report an Our results suggest a hypothesis that we need to increase knowledge about elderly longevity because data in the literature show that elderly people have a biology and a set of risk factors for cardiovascular diseases different from those in E3E3 individuals, suggesting the influence of additional environmental factors that were not analyzed in this study (such as nutritional condition) or interaction with other genetic factors that could reduce or neutralize negative effects caused by the E4 allele or reduce beneficial effects of E3E3 genotype (such as high uric acid levels). Complementary studies on the relation to other genetic polymorphisms in this population may help to elucidate this hypothesis.

Regarding individuals with longevity, the prevalence of cardiovascular diseases is very high, and the proportion of these people will significantly increase in the next decades. Future studies determining a pattern of cardiovascular risk for this age group will be paramount, both for preventing and handling these risks. Therefore, studies on the polymorphism of genes associated with cardiovascular diseases should be carried out comparing populations of individuals with heart disease with populations of healthy individuals and also individuals above the age of 80 years.

Acknowledgement: Our results allow us to conclude that, even though no individual with the E4E4 genotype existed in the population studied, suggesting a frequency-dependent effect, no association with risk factors and morbidity related to cardiovascular diseases in heterozygous individuals was observed. This happened even with LDL-c levels significantly higher than those observed in individuals with the E2 allele. We thank the Coordenacao de Aperfeicoamento de Pessoal (CAPES), Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq),I'm sorry, but the provided text is too short and lacks context. Could you please provide me with more information or text to work with?