Association study: Although GWAS does not rely on choosing the right gene beforehand, they do require a large sample size to support with adequate power the hundreds of thousands of SNPs.

Table Associated with Longevity in SNPs SICS LLIs SNP CHR Gene Assoc Model a/A MAF p Value OR (95% CI) rs2495513 1 TMEM61 AM G/A 0.2 3.98E-05 0.60 (0.47-0.77) rs4291539 1 DM C/T 0.42 2.37E-05 1.81 (1.37-2.39) rs10923806 1 RM G/A 0.38 7.24E-06 2.30 (1.59-3.33) rs1538287 1 KCNH1 DM A/G 0.18 2.64E-05 0.55 (0.42-0.73) rs6540664 1 AM A/G 0.5 4.45E-05 1.46 (1.22-1.75) rs6701445 1 TAF5L DM T/C 0.15 2.24E-05 1.84 (1.39-2.45) rs12088486 1 DM A/C 0.28 8.83E-06 0.56 (0.43-0.72) rs10514626 1 AM A/G 0.06 4.65E-06 0.38 (0.24-0.58) rs7583529 2 CFLAR RM A/G 0.21 6.45E-05 3.15 (1.75-5.68) rs4505466 2 SH3BP4rs10956502 8 FAM49B DM G/T 0.31 2.25E-05 0.57 (0.44-0.74) 
rs2905476 9 RM T/C 0.28 6.35E-05 0.29 (0.15-0.55) 
rs7873259 9 ANKRD19 RM G/A 0.27 1.18E-05 3.14 (1.84-5.37) 
rs4740391 9 AM G/A 0.11 3.99E-05 0.52 (0.38-0.71) 
rs12413082 10 MSRB2 DM T/C 0.32 1.50E-06 0.53 (0.41-0.69) 
rs7915479 10 CDH23 DM T/C 0.47 1.47E-05 1.92 (1.43-2.59) 
rs1556758 10 SORCS1 RM C/T 0.45 3.47E-05 0.50 (0.36-0.70)
rs2073586 11 ABCC8 RM T/C 0.4 8.15E-05 0.48 (0.33-0.69) 
rs11237644 11 AM A/G 0.4 9.24E-06 1.52 (1.26-1,83) 
rs870959 11 AM T/C 0.40 1,27E-05,1,51 (1,25–1,81) 
rs6592810,11 AM C/T,33,1,10E−05,1,54,(1,27–1,86) 
rs4938180 11 IGSF4 RM T/C 0.49 2.16E-05 1.91 (1.41-2.58) 
rs2111173 12 PTPRO DM C/T 0.39 5.22E-05 1.74 (1.33-2.28) 
rs969845 12 RM A/G 0.18 7.22E-05 5.87 (2.19-15.70) 
rs9315385 13 DCAMKL1 AM G/T 0.14 3.98E-05 1.74 (1.33-2.26) 
rs2147556 13 AM T/C 0.29 9.59E-06,0,63,(0,52–0,78) 
rs731287 13 RM T/C 0.28,9,88E-05,2,51,(1,56–4,05) 
rs285097,13 RNF113B AM G/T,0,08,3,79E-05,0,47,(0,33–0,68) 
rs1584547 14 RM T/C 0.24 8.52E-05 2.96 (1.69-5.21) 
rs4594173 14 MADMC1 AM G/A 0.08 4.88E-05 1.94 (1.40-2.69) 
rs2277472 14 MAMDC1 AM T/C 0.09,3,88E-05,1,95,(1,41–2,70) 
rs10134056 14 RM A/G 0.39 5.61E-05 0.46 (0.31-0.68) 
rs4777170 15 RM C/T 0.43 4.52E-05 2.00 (1.43-2.80) 
rs81647 16 DM G/A 0.4 5.53E-05,0,58,(0,44–0,76) 
rs1582594 16 DM A/C 0.32 3.00E-06,1,87,(1,44–2,43) 
rs2967137 16 DM C/T 0.37 8.00E-06,1,83,(1,40–2,38) 
rs6504441 17 PRKCA AM T/C 0.3 1.06E-06,0,60,(0,49–0,74) 
rs2070325 20 LPLUNC4 RM G/A 0.3 5.98E-05,2,42,(1,56–3,77) 
rs130329 22 RMCAMKIV and longevity in Southern Italian Centenarians. Our findings suggest that the CAMKIV gene plays a prosurvival role by inducing the phosphorylation of SIRT1, AKT, and FOXO3A. This mechanism promotes cell cycle arrest and oxidative stress resistance, which are key factors in aging and longevity. 

To investigate the effect of rs10491334 on CAMKIV protein expression, we compared mononuclear cells from homozygous carriers to those from individuals carrying the wild-type gene. We found that the protein level of CAMKIV is significantly lower in homozygous carriers. Additionally, our study revealed a higher frequency of wild-type CAMKIV genotypes among LLIs, supporting a prosurvival role for CAMKIV.

Our results suggest that CAMKIV could represent a potential target for fighting aging-associated illness. Further studies are needed to fully understand the molecular mechanisms underlying the association between CAMKIV and longevity.genome-wide association studies. Nat Genet 2006;38:904-909.

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