significantly increased 1 }in ON| the | - male « - centenarians « of 1 MOO ND A AAS‘northern | J weeItaly | <’4 VS | ’= in | aeepe. the « Een er: control EEsamples), « eeeour | study » failed | | to. | show | any , & haplogroup | <@(cid:9)(cid:15) ,> Re Meee) association | | with « either | age eeegroup ( ) OF § so Meeer gender. | .Inf yoo1 fact, \ , We ( - observed < eedlas ye EMPslight « eee srt decrease 1 (A>int —" br OS1 the ff Bee ee> frequency « / of t f the J & IRENE OS| haplogroup o”in | “wrthe | oNaged | samples | <+’=> ) compared | | to | ‘cothe « control | samples | <+*=>(cid:28) 2 nsThrough | opethe | on- phylo- EOSgenetic  oeaE- analysis | “Pew \ ame; however 1 r LWO ¢ ee eeedistinct | t branches s were § _ eee ee- shown t eee| to f KN SESE» form t a\ the . &  eo ow Pee| haplogroup.   first | t branch ( we) eee (hap41, | , hap 18, | , hap27, | , hap36 ¢ y and | (cid:6)(cid:10)(cid:15)4+> ) is ¢ ; defined | | by 1 the | CEN EOP OE> presence ¢ ao”> of t “MrF the | » Hint  ee NN OEE| restriction : | site +34:;(cid:21) eg nasrand ¢ AP OOP| displays « “res5 an I IE IIE| increased { OF ON ODEN| frequency 1 int mae\ the wii pos> aged s | samples (    +((cid:3) compared to the. somcontrols    ress *1), pe eeee ee”while the ee ETsecond tee FORbranch BN ES(hap5, oN eethap23 “pee NSand Mehap6) aeis NETdefined | l byt ' the < - absence « > of t F the s > site | +3((((cid:21) rand | | has < 5 a ( 1 decreased | ee Meee pe ey| frequency i “royint 1 the aged « eps ae1 cohort of LE AS DOsamples |    %(cid:3) compared | to | the | weer eeecontrol | samples |    +4(cid:3)(cid:1) peWhen Jn BE| statistical ; analysis was | Vee eee.carried Ae| out « ton =e| the | Oe ES- frequency « differences » Nk een XN; P-values « Aeof ((cid:28)(+% feos aeand ((cid:28)(++ were ¢ EE LEELA J OIE- calculated for t r the | - first | t branch ( <+34:;(cid:21)> ) and t Pee| the : : second | | branch ( <+3((((cid:21)>(cid:30) , respectively. | . Even after 1 the level « | of s DRED DDE RAIMA EED NEf significance \ > WaS ¢ “ose; adjusted, i ,int | this « s case | > by j een NX/ just t t two « MMe ENN pene’) comparisons ; sas t aye; the & | haplogroup has | “Eeealready | been ON IO IE IISlongevity-associated, | using MSF Jee MN—Bonferroni’s | EE IEEcorrection | <’ < ((cid:28)(’%>(cid:30) oN erthese P-values —— ireremain | eNO Naysignificant. NELLIS II IIISUnfortunately ‘o™in the Italian study they  ~ MMe Itested CAD MAMfor the defining » PELE LILES ODErestriction | ofsite, +3(3%(cid:21)(cid:30) for: the . & FEN IEhaplogroup -and | it: is) ee Oetherefore | oenot | possible to NEE IIISascertain which branch their ~or oe Meindividuals eee oeassigned “Pr Ssto the & haplogroup eee EESpredominantly belonged (De Benedictis et al., +;;;>(cid:28) It is OE EItempting to BN EEE Especulate OTN MEthat the Italian Demale EEE IIE NEcentenarians t s used i lint 1 their : oo neer study | "9 A =fall AeA)lin t ase a1 to t ) the { > first | nee pee m OS”t branch, | “~— ePeee, given t 1 the i - increased | | prevalence « > of this i sin t \ the | > Irish « | aged EE OLIN)| subjects, ; “Joeand t | this « s could « | explain \ ENED1 why t or, *’ their « ‘ observation ¢  b*1 of i eeFa s | significant increase i int | the | oo» broad . & NS J Es ee| haplogroup i yin | 1 their « cae aN‘aged : ‘pee ND| subjects ‘yee> WaS I >not! t reflected i lint 1 the | “oe» Irish < 1 aged subjects.

oe JeeIntriguingly 1 the | - polymorphic | restriction : site, +34:;(cid:21)(cid:30) _ differentiating 1 the 1 - first | - branch « | of the . &   OO I| haplogroup |  iS 1 pw dS; reported | as | : the - ‘mutation’ | that “sooodefines 1 the | 1’    which | is ; a ee eeesubgroup of rethe ae apsbroad African haplogroup 1 < “hen et al., +;;%>(cid:28) ot — eeThis obo eeemutation was ee ee NEobserved | fat ;  | frequency | of | %= <++ out | ofof | ’’;> uM)in | the | Trish 4 meeeg A A fe”| population | | examined | | but) | Was restricted 1 | to 1 ) the | oe- first | i” aint branch « \ of t wa‘the | & NN RA PODS| haplogroup. | DEE EEE LEO” ET. Interestingly, 1 _ the ] 1’ - haplogroup | ) has ; also been 1 reported 1 lint 1 the | > Italian | 1 population ( Sree ore «bs fee”1 (Quintana-Murci « eSi et < Os?tal., | +;;;> ) at ¢ “ettat “revo b *| frequency « of | +4= <’ out | cPt of +%>(cid:30) . which i 1 is «  “Pere”; considerably |   ahigher 1 ‘than t that 1 OE OID| reported | 9 eS| here. ( se. Given 1 oe\ the | we- significance eeeattributed 1 ase| to | fo Naethe . & | haplogroup | in | eee FEE Oe ™_ longevity, ; it) | would | so| be « re*of § great 1 interest | | to 1 EN EEE) re-examine | - the individuals - used in | “peethe conserstudy | by meee swine do ** omBenedictis | et cal. <+;;;> and | establish | | whether | they have 1 > this 1 ; mutation. ° . The eas YO- importance > of t F the « - overlap | vu RR NE) between a oe\ the two l ) mutations « ; defining 1 r the