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In conclusion, we present new candidate variations for human longevity located in the DNA damage signaling and DNA repair pathways and the GH/IGF-1/INS signaling and pro/antioxidant pathways. We found that four of the 17 identified SNP associations showed significant and similar direction of effect when using the two different study approaches applied to the Danish discovery cohorts, while two SNP associations showed similar tendencies. However, none of the 17 SNP associations displayed replication in the independent samples from Germany and the Netherlands, although for two SNP associations some support was noticed.

Conflicts of interest: The authors have no conflicts to declare.

Acknowledgments: This study was supported by the Max-Planck Institute for Demographic Research (Rostock, Germany), the INTERREG 4 A programme Syddanmark-Schleswig-K.E.R.N (by EU funds from the European Regional Development Fund), the National Institute on Aging (P01 AG08761), a Dutch grant from the Netherlands Consortium for Healthy Aging (Grant 050-060-810) in the framework of the Netherlands Genomics Initiative/Netherlands Organization for Scientific Research (NWO), the European Union's Seventh Framework Programme (FP7/2007-2011) under grant agreement n 259679, the Novo Nordisk Foundation, the Aase and Ejnar Danielsen Foundation, the Augustinus Foundation, the Brdrene Hartmann Foundation, the King Christian the 10th Foundation, and the Einer Willumsens Mindelegat Foundation. The Danish Aging Research Center is supported by a grant from the VELUX Foundation. Susanne Knudsen, Steen Gregersen, Ulla Munk, Shuxia Li, Anne Mette Hedegaard Nielsen, Marlene Graff Srensen, and Lene Elnegaard are thanked for excellent technical work.

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