, Results

/*4* Forty-six | /mtDNA | haplotypes

Restriction « lenzyme ¢ » analysis « sof t F the 7 ’3*4 } bp | /   - amplified | | product | <(cid:17)(cid:3)+4;(:0+3%%+> ) of > mtDNA « NN NEE JOD”. derived 1 | from t \ the ‘ ’’; ) Trish 1 ES Meeeindividuals seers used i lint oe\ this : s study 1 ee Meee aNrevealed lat \ total was| of | 4* restriction : | Sites. | . Sequencing : r analysis 1 : identified | that { wet five > of t f these \ > Were I novel, ‘unreported 1 RE OED ENT| restriction : “pee1 sites, ¢ oh, and 1 eee| the oF ee eespecific 1 - nucleotide « > changes sare § given i in t : the mee Dd| to’ ‘J eee Pb» Table | 4(cid:28)  total « | of ¢ *3 ————rrorodifferent | ae NE OO| genetic | haplotypes « NE NEES IIA ED EE; encompassing | “™ &*the >Toentire | ’’; ; involved i [in | | this : ; study) were i ESidentified « | on t aethe | oJ}basis of 1 the | EE EO- presence | <+> ) OF § ‘absence mere) of « F each « 1 of t F the : 4* LD| restriction s 1 Sites ( ; (Table : 4>(cid:28) . Eleven ¢ of t f the | eR poe”> haplotypes whee NA> WETE | it see ee> unique t > tot ) the cohort | of | ‘samples | and | 5 wane ha| twenty | ee fe ewhaplotypes » , were | BEN ETE- unique | hte”to! arthe ; aged | SO| sample | - group 4>(cid:28) _ The | NE EEE TEE- frequency « NO Jdifferences | Jere; found | us peo| between | \ the 1 ——two 3 )age | eee SS: groups | oSfor | a |‘ the *3 oer) haplo- meee fe ane; (Table - *> ete) failed 1 oN| to 1 Peetey reach : | statistical : | significance ' - when : ™ “or of| analysed | ore| by | ’ the | - Fisher’s « peexact wee hae ee(data | | not Fee ™: shown). ' _ These 1 results | » WeTe « ween”also ) examined ' | with 1 PO| respect | “7 *tol » male ; and | | female DENN IEE ND NEE T®differences | ; between t 1 the ¢ > control i | and < | aged s | sample s BE AREER EASE> groups i re." *sand ¢ | again 1 1 none « of t f these ee OS‘found | | to 1 ) reach a | | level   * statistical ee “oN eeesignificance | mere &after oereeeecorrection : | for | * the « *3 ) multiple  +  ((cid:1);%+(cid:2)*3(cid:3) ’ << ((cid:28)((++(cid:28) enThis | is | » hardly : surprising - taking i into ; ee) account “Peevery | ueneny ™high | number | weof a rnvariables | <*3 haplotypes) with» a nrespect | Besto | “oothe | mee GD REsample size | <’’; as well ; | as | the | low van NE eesfrequencies « “21of | ‘the 1 1f NWA ND/ majority } “pe.of | haplotypes.

Table : ’

—The | a Ne OR: polymorphic | meen INS- nucleotide i is | “_—_ ree: italicised. | . Positions « ; defined | according | tot ) the | : published 1 | mitochondrial | | genome AA pee eessequence ¢ es> of 1 pees eeef Anderson ¢ 1 et i t al. ( <+;:+>

 Probe Position Conc. ( <(cid:15)(cid:1)>  Wash t 1 temperature ( <(cid:2) > 5'sequence  4(cid:4) Mito | + %+,(0%+:3 ’( *3 GAAAC  CAAG (   » TAAC (cid:14).@(cid:14) Mito : 4 %+,(0%+:3 ’( *3 GAAAC CAAG ¢ + | TAAC (cid:14).@(cid:14) Mito ¢ ’ ;(*:0;(3’ ’( %( .@@(cid:14)(cid:14)@ $ CAC . Mito ¢ * ;(*:0;(3’ ’( *: .@@(cid:14)(cid:14)@ + CAC .

) SUONVUA aprogonu SuIMOTJOJ oy) UTeIUOS pue poyodar A[snorAoid uss JOU sARY YSIYM ‘sures ops ul SuNtnsoad [[e ‘Apnys sry) ur aey poyNuopreat ATAA Trimate este) «nangindsad nerd wamingt am om marenn fact den) cad finden wren wm AnTHAn wm cyte nanarnert adem fiat



)*+* . Ross ¢ yet ¢ tal. / , / Experimental ( | Gerontology - /0 123345 4404644(cid:30)7



++,( )*+* . Ross ¢ yet ¢ tal. / , / Experimental ( | Gerontology - /0 123345 4404644(cid:30)7 y1geL sdnois a8 yjoq Joj paasasgo sarsuanbayy adXyojdey oy,AInvT Ajuo sadXojdey asoyp syiodar a[qey oy) JO WoNog ey] ‘sofdures Jo WOYOS pase oy) pue [oNUOS sy) YO UT poAsosqo sodXyojdey asoy) smoys e[qei oy) JO yey doy oy,odn are? ASp man iat naamean carnanharn adfiarden ant [P10] pourquIos oy} YIM J9Y}930) padnoss are oy) Woyed Aouonbay sures oy) pey edAjo] dey suo uey) o10ur asoym pur sojdures pose oy) Jo [ONUOS oY} JoY}10 UT poarosgoCn ead Gaiden acam eindat aap. arn Tamanna ant ‘eardiipe ta awanas naSp ain nie Tania, am mana nm nasmena eadfiaiden acam eunne arapy am ia upn daa ant povodara TACaA, 6TI = 4 ‘pasy OOL = ¥ ‘JonuOD  adAjojdey 671 = 4 (%) [OL 06 = ¥ aeUto.y 6€ = ¥ OPIN OOT = ¥ (%) TOL 6S = ¥ opeulo.y Ip = 4 oe  >’+<%+*++>3<344+(cid:15)(cid:10)9 >34<,*44*+>’*<’*4’;+’(cid:15)(cid:10)9 >,<;,’>’+<’+;4*(cid:15)(cid:10)9 >4<*4+>:<:**%(cid:15)(cid:10)9 >+<++A>*<*4+3(cid:15)(cid:10)9 >3<:%4>%<%*+,(cid:15)(cid:10)9 >+<++A>+<++A:(cid:15)(cid:10)9 >+<++A>+<++A;(cid:15)(cid:10)(cid:6) >+<++A>+<++A(+(cid:15)(cid:10)(cid:6) >’<4+’>’<’A’’+(cid:15)(cid:10)(cid:6) >4<**A>’<’++4+(cid:15)(cid:10)(cid:6) >%(cid:28)+<’A’>+<++A*+(cid:15)(cid:10)(cid:6) >%(cid:28)+<’++>+<+A+,’(cid:15)(cid:10)(cid:6) >%(cid:28)+<’A’>+<+A+:’(cid:15)(cid:10)(cid:6) >+<+A+>+<++A;’(cid:15)(cid:10)(cid:6) +(+’,;’::’%34(cid:11)(cid:10)(cid:3)(cid:4). M408a1D9 a8p Aayyia of Saspo anbiuy) JonuogC1082:psy adMjojdey7 anbiun  jeIoL ayeula,y oe [r10, .L ayeula,y arnnasw jonuo> *A+3’(cid:30)%’(cid:30);+(cid:30)4(cid:15)(cid:10)(cid:6) ’++++(cid:15)(cid:10)(cid:6) 3+A*’(cid:30)4’(cid:30)’’(cid:30)+’(cid:30)(’%+(cid:15)(cid:10)(cid:6) (++A(cid:30)%4(cid:30)*4(cid:30),+(cid:30)3+(cid:15)(cid:10)(cid:6) 3*(cid:30)%*(cid:30)**(cid:30)+*(cid:30):4(cid:30),4 3+’’4(cid:30):+(cid:15)(cid:10)(cid:6) ’A’(4(cid:15)(cid:10)(cid:6) *’A4*(cid:30)+4(cid:15)(cid:10)(cid:6)  * rcdreu *A+’*(cid:30)(*(cid:30);4 ’’A34(cid:15)(cid:10)(cid:6) :’:+(+’+,%(cid:11)(cid:10)(cid:3)(cid:4).

/*2* Phylogenetic « analysis