2b. FBAT, Top 5 p-values by Phenotype*

Trait SNP Chromosome Physical location GEE p-value FBAT p-value Gene Region (within 60 Age at death rs10493513 1 73,091,610 0.640 1.5 × 10-6 rs10493514* 1 73,092,533 0.623 2.8 × 10-6 rs6689491* 1 73,064,050 0.205 2.0 × 10-5 rs10493515 1 73,527,652 0.225 2.3 × 10-5 rs10493518* 1 73,572,652 0.191 3.6 × 10-5 rs10493517* 1 73,570,372 0.215 4.2 × 10-5 rs6657082* 1 73,065,349 0.224 5.5 × 10-5 rs10498263 14 19,285,288 0.310 8.3 × 10-5 OR4Q3 OR4M1 rs1915501 4 28,612,632 0.383 1.1 × 10-4 rs1405051* 1 73,060,505 0.176 1.4 × 10-4 rs6459623 6 18,634,791 0.604 1.5 × 10-4 IBRDC2

Table 2: Aging and Longevity Phenotypes† for FHS 100K Project: Results of Association and Linkage Analyses (Continued)

Morbidity-free survival at age 65 rs10509200 10 65,296,567 0.613 7.0 × 10-5 rs965036 6 20,099,022 0.550 8.6 × 10-5 rs720565 6 136,834,657 0.014 9.8 × 10-5 MAP7 rs1192372 2 84,923,204 0.094 9.9 × 10-5 rs10505239 8 115,976,403 0.141 1.3 × 10-4 Age at natural menopause† rs959702 10 2,139,260 0.003 1.4 × 10-5 rs7165378 15 69,478,558 0.006 6.4 × 10-5 rs997161 10 130,876,127 0.074 8.6 × 10-5 rs165284 1 91,235,574 0.006 8.6 × 10-5 ZNF644 rs2280585 3 64,882,324 0.884 1.1 × 10-4 Walking speed exam 7 rs4471448 11 86,760,972 0.175 3.8 × 10-6 TMEM135 rs336963 5 83,005,460 0.570 2.6 × 10-5 HAPLN1 rs7862683 9 18,257,947 0.001 4.9 × 10-5 rs9317757 13 68,458,430 0.252 7.8 × 10-5 rs10501636* 11 86,789,967 0.355 1.1 × 10-4 rs2340392 3 80,440,990 0.002 1.7 × 10-4 Biologic age by osseographic scoring system rs1380703 2 57,852,938 0.008 1.1 × 10-5 rs324702 4 77,094,969 0.390 3.3 × 10-5 PPEF2 rs324735 4 77,062,193 0.096 7.6 × 10-5 rs1106184 2 10,914,125 0.006 8.8 × 10-5 PDIA6 rs604578 18 30,923,438 0.004 9.5 × 10-5 MAPRE2 2c Linkage¶ Peaks with LOD scores ≥ 2.0 Trait SNP closest to Chromosome Physical location 1.5 – LOD 1.5 – LOD LOD score linkage peak support support interval start interval end Age at natural menopause rs1371217 4 182,890,808 178,671,796 186,905,362 2.08 rs10509024 10 56,567,832 36,084,470 70,573,011 2.39 rs4793513 17 66,429,892 60,635,492 69,512,021 2.48 Walking speed, exam 7 rs2769261 1 113,278,949 107,080,550 144,332,709 2.30 rs921055 2 233,522,842 229,328,008 242,141,304 2.13 rs2602044 3 109,427,883 102,255,525 111,604,019 3.38 rs8011773 14 96,927,485 96,342,135 100,389,787 2.69 rs1362626 16 4,489,227 205,160 10,344,522 2.05 Biologic age by osseographic scoring system rs353810 9 86,258,745 81,441,976 92,220,168 3.26 rs1203981 16 205,160 205,160 7,431,239 2.49 rs2248383 21 35,151,811 27,412,716 40,940,879 2.22

Morbidity-free survival at age 65 rs10509200 10 65,296,567 0.613 7.0 × 10-5 rs965036 6 20,099,022 0.550 8.6 × 10-5 rs720565 6 136,834,657 0.014 9.8 × 10-5 MAP7 rs1192372 2 84,923,204 0.094 9.9 × 10-5 rs10505239 8 115,976,403 0.141 1.3 × 10-4 Age at natural menopause† rs959702 10 2,139,260 0.003 1.4 × 10-5 rs7165378 15 69,478,558 0.006 6.4 × 10-5 rs997161 10 130,876,127 0.074 8.6 × 10-5 rs165284 1 91,235,574 0.006 8.6 × 10-5 ZNF644 rs2280585 3 64,882,324 0.884 1.1 × 10-4 Walking speed exam 7 rs4471448 11 86,760,972 0.175 3.8 × 10-6 TMEM135 rs336963 5 83,005,460 0.570 2.6 × 10-5 HAPLN1 rs7862683 9 18,257,947 0.001 4.9 × 10-5 rs9317757 13 68,458,430 0.252 7.8 × 10-5 rs10501636* 11 86,789,967 0.355 1.1 × 10-4 rs2340392 3 80,440,990 0.002 1.7 × 10-4 Biologic age by osseographic scoring system rs1380703 2 57,852,938 0.008 1.1 × 10-5 rs324702 4 77,094,969 0.390 3.3 × 10-5 PPEF2 rs324735 4 77,062,193 0.096 7.6 × 10-5 rs1106184 2 10,914,125 0.006 8.8 × 10-5 PDIA6 rs604578 18 30,923,438 0.004 9.5 × 10-5 MAPRE2

2c Linkage¶ Peaks with LOD scores ≥ 2.0

* For each phenotype SNPs are ranked by p-value. A SNP in LD (r2 > 0.8) with a higher ranked SNP, is identified with an asterisk. All SNPs for a phenotype are listed until 5 independent SNPs are identified. Thus, for some phenotypes more than 5 SNPs are listed. For the age at death trait, the FBAT analysis identified two areas on chromosome 1 in LD, with r2 = .5–.6 between the two regions and r2 of nearly 1.0 within the region. † Multivariable-adjusted trait results are presented ‡Trait had <500 participants in the sample.

Table 3: All Significant SNP Associations (GEE or FBAT p-value < 0.01) for at least Two Traits

FBAT: Age at Death and Morbidity-Free Survival at 65 years

Trait 1 Trait 2 SNP Chr Physical Gene Trait 1 GEE Trait 1 FBAT Trait 2 GEE Trait 2 FBAT Position p-value p-value p-value p-value Age at Morbidity- rs6682403 1 234,743,324 0.849 0.004 0.106 0.004 Death free at 65 rs10488907 4 113,669,709 ALPK1 0.452 0.008 0.336 0.009 rs17190837 9 13,391,548 0.010 0.009 0.097 0.004 rs4752977 11 47,257,005 MADD 0.736 0.008 0.002 0.009 rs10506274 12 80,103,932 0.531 0.001 0.989 0.001 rs2831154 21 28,059,331 0.323 0.008 0.358 0.006 rs243725* 21 28,060,803 0.264 0.007 0.359 0.008

*r2 > 0.80 with the preceding SNP

3b. GEE: Age at Death and Morbidity-Free Survival at 65 years