*r2 > 0.80 with the preceding SNP

3b. GEE: Age at Death and Morbidity-Free Survival at 65 years

Trait 1 Trait 2 SNP Chr Physical Gene Trait 1 GEE Trait 1 FBAT Trait 2 GEE Trait 2 FBAT Position p-value p-value p-value p-value Age at Morbidity- rs9308261 1 113,603,160 MAGI3 0.009 0.156 0.002 0.900 Death free at 65 rs10490518 2 31,223,004 GALNT14 0.009 0.373 0.010 0.948 rs2374983 7 94,516,375 PPP1R9A/ 0.006 0.980 0.007 0.727 PON1 rs655883 11 98,994,584 CNTN5 0.006 0.390 0.001 0.293 rs1368850 11 130,433,518 0.004 0.387 0.005 0.292 rs4943116 13 32,995,650 STARD13 0.006 0.205 0.008 0.049 rs2254191 13 45,344,403 0.007 0.425 0.004 0.116 rs1620210 13 45,759,488 C13orf18 0.001 0.161 0.004 0.068 rs2823322 21 15,814,903 0.0004 0.045 0.006 0.029 3c. FBAT: Biologic Age and Walking Speed Trait 1 Trait 2 SNP Chr Physical Gene Trait 1 GEE Trait 1 FBAT Trait 2 GEE Trait 2 FBAT Position p-value p-value p-value p-value Biologic age Walking speed rs873348 4 178,246,509 0.135 0.004 0.114 0.006 Biologic age Walking speed rs10520361* 4 178,247,037 0.074 0.006 0.054 0.005 Biologic age Walking speed rs31564 5 135,258,152 IL9 0.015 0.001 0.008 0.002 Biologic age Walking speed rs1862345 5 148,018,498 HTR4 0.172 0.0002 0.399 0.008 Biologic age Walking speed rs7844834 8 11,323,556 C8orf12|C8or 0.017 0.004 0.011 0.004 f13 Biologic age Walking speed rs952658 12 20,756,568 SLCO1C1 0.024 0.008 0.935 0.006 Biologic age Walking speed rs6487366 12 23,994,617 SOX5 0.017 0.003 0.959 0.004 Biologic age Walking speed rs7135493 12 28,134,847 0.020 0.006 0.033 0.004 Biologic age Walking speed rs10492036 12 124,728,934 0.308 0.005 0.169 0.009 Biologic age Walking speed rs1978945 13 105,641,257 0.093 0.009 0.031 0.006 Biologic age Walking speed rs2165723* 13 105,641,610 0.046 0.010 0.027 0.003 Biologic age Walking speed rs10492651* 13 105,641,634 0.083 0.009 0.023 0.001 Biologic age Walking speed rs9301112* 13 105,642,018 0.097 0.004 0.053 0.003

3c. FBAT: Biologic Age and Walking Speed

* r2 > 0.8 with the preceding SNP (calculated if the distance is <250,000 base pairs)

3d. GEE: Biologic Age and Walking Speed

Trait 1 Trait 2 SNP Chr Physical Gene Trait 1 GEE Trait 1 FBAT Trait 2 GEE Trait 2 FBAT Position p-value p-value p-value p-value Biologic Age Walking speed rs1474827 6 134,886,011 0.007 0.746 0.000 0.233 Biologic Age Walking speed rs10231641 7 119,166,342 0.004 0.077 0.009 0.278 Biologic Age Walking speed rs310575 8 51,603,257 SNTG1 0.008 0.209 0.003 0.007 Biologic Age Walking speed rs10520603 15 84,170,955 0.009 0.008 0.003 0.050 Biologic Age Walking speed rs7166323* 15 84,171,745 0.009 0.012 0.003 0.065 Biologic Age Walking speed rs2215921 16 9,604,834 0.007 0.049 0.001 0.316

* r2 > 0.8 with the preceding SNP (calculated if the distance is <250,000 base pairs)

4: All Significant SNP Associations with Selected Longevity Candidate Genes* (FBAT or GEE p-value < 0.01)

Trait Gene SNP Chr Physical FBAT p-value GEE p-value SNP function SNP position relative Position to gene (up to 60 kb) Age at death FOXO1a rs4943794 13 40,071,408 0.068 0.00028 Intron in rs10507486 13 40,084,501 0.043 0.00013 Intron in GAPDH† rs4764600 12 6,472,241 0.833 0.005 Locus/intron near KL rs683907 13 32,522,175 0.009 0.507 Intron in rs687045 13 32,522,889 0.007 0.712 Intron in LEPR rs1475398 1 65,695,278 0.069 0.005 Untranslated in rs1343981 1 65,757,349 0.031 0.006 Intron in rs10493379 1 65,757,948 0.015 0.004 Intron in rs2154380 1 65,769,462 0.004 0.003 Intron in rs6669117 1 65,773,093 0.050 0.007 Intron in PON1 rs2374983 7 94,516,375 0.980 0.006 Intron near PSEN1 rs362356 14 72,708,382 0.005 0.130 Intron in SOD2 rs911847 6 160,039,379 0.358 0.005 Unknown near WRN‡ rs2543600 8 30,969,282 0.182 4.2 × 10-6 Unknown near Morbidity-free survival at age 65 GHR rs719756 5 42,761,386 0.003 0.676 Unknown near LEPR rs1171278 1 65,700,167 0.042 0.003 Untranslated in rs3790426 1 65,755,040 0.460 0.002 Intron in MORF4L1 rs1383636 15 76,893,275 0.458 0.007 Unknown near PON1 rs2374983 7 94,516,375 0.727 0.007 Intron near rs854523 7 94,542,884 0.850 0.007 Intron in PTH rs10500784 11 13,530,401 0.010 0.990 Unknown near WRN‡ rs2725369 8 30,970,566 0.113 0.003 Unknown near Biologic Age by OSS FOXO1a rs1923249 13 40,041,881 0.006 0.004 Intron in rs4943794 13 40,071,408 0.009 0.016 Intron in HSPA9 rs256014 5 137,930,983 0.101 0.005 Intron in LASS6 rs1002666 2 169,303,525 0.001 0.008 Intron in SOD2 rs911847 6 160,039,379 0.024 0.009 Unknown near TLR4 rs1927914 9 117,544,279 0.007 0.401 Locus near Walking speed ESR1 rs9322361 6 152,551,257 0.124 0.0089 Intron in LASS6 rs6433083 2 169,324,821 0.232 0.006 Intron in NR3C1 rs2918418 5 142,703,566 0.005 0.081 Intron in rs10515522 5 142,738,587 0.004 0.084 Intron in SOD1 rs2833485 21 32,000,796 0.008 0.507 Locus/intron in TERF2 rs728546 16 68,013,029 0.0045 0.533 Unknown near FASLG rs6700734 1 169,362,468 0.003 0.029 Intron in

*79 genes identified from NCBI, SAGE ke, and GenAge databases; 12 genes with no SNPs on 100K chip; 67 genes with 1–45 SNPs on 100K chip; LGV1 2036 SNPs on chip, results for this region available on the web †The most strongly associated SNP near GAPDH is actually closer to MRPL51 ‡The most strongly associated SNP near WRN is actually closer to PURG

related phenotypes and 100K SNP genotyping results available on the web. This resource has the potential to detect novel susceptibility genes for human longevity and aging and to examine the relevance of promising candi- date gene associations reported in animal models to human aging. We describe several strategies to prioritize SNP associations in this unique resource to enhance the discovery of various genes and pathways that contribute to the control of human longevity. Furthermore, FHS investigators are part of the NIA sponsored Longevity Consortium http://www.longevityconsortium.org which offers the opportunity of collaboration with other investi- gators to replicate important findings in additional cohorts.