How long should I allow the cells to migrate or invade?
我应该让细胞迁移或入侵多长时间？
For the best statistics, choose an assay time that results in untreated cells closing at least 2/3 of the original open area of the detection zone, but less than 100% closure. An advantage of the Oris Cell Migration and Invasion Assays is that you can inspect the experiment at any time during the incubation period to ascertain how far the cells have moved. The first time you run an Oris assay, simply observe the cells periodically under a microscope to assess the extent of migration/invasion and stop the experiment when the appropriate degree of movement is observed. Since rates of cell movement differ widely between different cell types, optimal incubation times will vary with varying cell types. Migration times can vary from 16 – 72 hours, while invasion times can vary from 1 – 6 days. For extended experiments, we recommend that you change culture medium above with fresh inhibitors every 48-72 hr.
为获得最佳统计数据，选择的检测时间应使未处理细胞的闭合面积至少达到检测区原始开放面积的 2/3，但闭合面积应小于 100%。奥利斯细胞迁移和侵袭检测法的一个优点是，您可以在培养期间随时检查实验，以确定细胞移动了多远。第一次进行 Oris 检测时，只需在显微镜下定期观察细胞以评估迁移/侵袭程度，并在观察到适当程度的移动时停止实验。 由于不同细胞类型的细胞移动速度差异很大，因此最佳培养时间也因细胞类型而异。迁移时间为 16 - 72 小时，侵袭时间为 1 - 6 天。若要延长实验时间，我们建议您每 48-72 小时更换一次培养基，并加入新的抑制剂。