Why are Oris assays better than Boyden chamber / transwell assays?
为什么 Oris 检测方法比 Boyden 室/经孔检测方法更好？
Oris assays provide a more physiologically relevant environment than Boyden / transwell assays because there is no artificial membrane through which cells must pass. Moreover, cell movements can be monitored in real time in Oris assays, as there is no membrane to interfere with microscopic observation. This not only provides more information, but also accelerates methods development because your end point is evident from simply observing the assay. The Oris 3D Embedded Invasion Assay is superior to Boyden / transwell assays for cell invasion because the cells are embedded in 3D throughout the assay, whereas cells are seeded on a 2D surface in Boyden / transwells assays, which changes cell physiology significantly (e.g. references 1,2,3,4,5,6,7,8).
与 Boyden 或 transwell 试验相比，Oris 试验提供了更贴近生理的环境，因为没有人工膜供细胞通过。此外，由于没有膜干扰显微镜观察，在 Oris 试验中可以实时监测细胞运动。这不仅能提供更多信息，还能加快方法的开发，因为只需观察试验就能发现终点。 在细胞侵袭方面，Oris 的三维嵌入式侵袭检测法比 Boyden / transwell 检测法更胜一筹，因为在整个检测过程中，细胞都是嵌入在三维环境中，而 Boyden / transwell 检测法是将细胞播种在二维表面上，这就大大改变了细胞的生理机能（如参考文献）。 1,2,3,4,5,6,7,8).