At what point can I add test compounds to Oris assays?
什么时候可以在 Oris 分析中添加测试化合物？
For Oris assays, add test compounds to the culture medium once the cells have attached and the stoppers have been removed, but before migration begins. For Oris Pro Assays, add test compounds once the BCG has dissolved and the cells have attached, but before migration begins. For both Oris and Oris Pro assays, adhesion time is dependent on cell line and plate coating, with attachment times. You may remove and replace the medium prior to adding test compounds in order to eliminate any non-adherent cells. In the invasion assays, compounds may be incorporated into the collagen I solutions and/or added to the culture medium above the collagen.
对于奥利斯检测法，在细胞附着并取下塞子后，但在迁移开始前，将测试化合物加入培养液中。 对于 Oris Pro 检测试剂盒，在卡介苗溶解、细胞附着后，但在迁移开始前加入检测化合物。 对于 Oris 和 Oris Pro 检测法，附着时间取决于细胞系和平板涂层以及附着时间。在加入测试化合物之前，可以移除并更换培养基，以清除任何不粘附的细胞。在侵袭试验中，可将化合物加入胶原蛋白 I 溶液中和/或加入胶原蛋白上方的培养基中。