Why (and how) is some serum heat-inactivated?
为什么（以及如何）热灭活血清？
Complement proteins naturally occurring in mammalian sera mediate cytolytic events, contract smooth muscles, and activate lymphocytic and myeloid cells.  Heat-inactivation destroys the activity of complement in serum.  This method is therefore recommended for immunological applications and for the culture of embryonic stem cells (ESCs), insect cells and smooth muscle cells.
The heat inactivation process must be carefully controlled to prevent denaturation of desirable serum proteins that are key constituents of sera necessary for cell and tissue propagation.
Heat inactivation occurs when serum is heated to 56 °C for a period of 30 minutes.
自然存在于哺乳动物血清中的补体蛋白可介导细胞溶解，收缩平滑肌，并激活淋巴细胞和骨髓细胞。  热灭活可破坏血清中补体的活性。  因此，推荐将该方法用于免疫应用和胚胎干细胞（ESC）、昆虫细胞和平滑肌细胞的培养。
热灭活过程必须小心控制，防止所需血清蛋白变性，所述血清蛋白质是细胞和组织增殖所必需的关键血清成分。
将血清加热至56 °C，持续30分钟，即可热灭活。