Why do cell and tissue cultures need serum?
为什么细胞和组织培养需要血清？
Serum is added as a supplement to culture media at a concentration of 2-10% to provide a mixture of nutritional, hormonal, growth and attachment factors. Serum also acts as a buffer to the cell culture system against a variety of disruptions to cell growth and toxic effects such as pH change, proteoloytic activity, or the presence of heavy metals or endotoxin.
以2-10%浓度添加血清作为培养基补充物，以提供营养、激素、生长和粘附因子的混合物。血清还可作为细胞培养系统的缓冲剂，可抵抗细胞生长过程中的各种破坏和毒性作用，如pH变化、蛋白水解活性或者存在重金属或内毒素。