Biodiversity maps can be a valuable way to initiate dialogue around biodiversity mainstreaming, providing a tangible view of biodiversity objectives and how these could be mainstreamed in a spatially explicit manner. Good biodiversity science needs to be transparent and defensible to meet the burden of evidence demanded during such discussions. When a biodiversity mainstreaming intervention is planned, or an opportunity arises, there is often existing scientific information available, which can be utilised or adapted as required. Pilot projects can often play an essential role, delivering on-the-ground experience and proof of application within the intended institutional context.