The legislation was consequently amended in 2011 to make a chemical method (EU-RL LC-MS/MS liquid chromatography-mass spectrometry) the new reference method, with a phasing out of the biological methods over four years. During this period a modified mouse bioassay is still permitted. Changes were introduced to the legislation dealing with treatment to kill viable parasites in fishery products for human consumption in order to update the requirements based on information provided by a recent opinion from The European Food Safety Authority (EFSA) on Parasites in Fishery Products and experience gained from the application of the present legislation.