Context : No clear date or monument can confirm the link between the two, but these Egyptologists presumed this by Ay's origins, also from Akhmin, and because he inherited most of the titles that Tiye's father, Yuya, held during his lifetime, at the court of Amenhotep III. Tiye was married to Amenhotep III by the second year of his reign. 
Subject : Tiye 
Relation : sex or gender
Which gender does Tiye belong to?