We observed the pancreas directly using US and could identify the thickest portion of the pancreatic tail. A 25-gauge EUS needle was inserted into the thickest portion of the pancreatic tail under US guidance. The needle was primed with 100% ethanol solution before the injection and 1.0 mL ethanol was slowly injected into the pancreatic parenchyma. Hyperechoic bubbles created by ethanol were continuously visualised during injection.