Cross-sectional CTL reformatted image in an obliqued dorsal plane set in a soft tissue window of a dog in group 2 at 1 min post-injection demonstrating the presence of an efferent lymphatic tract (arrow heads) coursing from the injection site (red dotted outline) to the right dorsolateral mandibular lymph node (star). See Supplementary Video 1 for corresponding percutaneous fluorescence using NIRF.