Tissue Doppler imaging (TDI) of male goat’s heart obtained from the left apical 4-chamber view at the septal and lateral mitral annulus (SMA, LMA). The sample volume was adjusted at 3.5 mm. The spectral velocity is expressed as systolic velocity (+, Sm) and two diastolic waves (E, Em; A, Am) which respectively evaluate the early and late diastolic mitral annular velocities.