Chest CT scan of a novel false-negative PC patient. A 44-year-old nonsmoking female with a ground-glass opacity in the left lower lung showed false-negative CrAg LFA detection in the lung aspirate after CT-guided percutaneous lung biopsy. No suitable pathological tissue was obtained and the patient eventually underwent thoracoscopic surgery and was diagnosed with PC. CrAg = cryptococcal capsular polysaccharide antigen, CT = computed tomography, LFA = lateral flow immunoassay, PC = pulmonary cryptococcosis.