The measurement was taken focusing on the best image for iATT without following the protocol for stiffness assessment. The two horizontal yellow lines graphically show the width (length of each line) and the length (distance between the two lines) of the iATT measurement area, which has a fixed size (length of 4 cm, from 35 to 75 mm from the skin). The yellow rectangle is the region of interest (ROI) for stiffness measurement. Because it is not possible to exclude the stiffness measurement, the stiffness ROI was intentionally positioned close to the liver capsule. This explains the huge variability between consecutive stiffness measurements with an IQR/M = 67% and a VsN always <50%.