
thymidine kines

thymidine kines anedi oka enjaim, idhi phosphotranspherase (oka kines): 2 '-dioxythymidine kines, eah. tii. pii-thymidine 5'-phosphotranspherase, ii. sii. <ii. dii. 1>. idhi chaalaa jeeva kanaalalo kanipistundi. idhi ksheerada kanaalalo rendu roopaallo untundi, tike1 mariyu tike2. konni vairaslu viral thymidine kinesla vyakteekarana kosam janyu samacharanni kooda kaligi untaayi.
thymidine kines praticharyanu utprerakam chestundih
ikkada thd anedi (dioxy) thymidine, ATP anedi adenosine trifasphate, TMP anedi (dioxy) thymidine monophosphate mariyu ADP anedi adenosine diphosphate.
dna samshlaeshanhaloo thymidine kineslu keelaka paatra poeshistaayi, anduvalla avi dnloki thymidini pravesapettadaaniki pratyaekamaina praticharya golusulo bhaagam kabatti kana vibhajanalo keelaka paatra poeshistaayi. aaharam nundi mariyu chanipoyina kanaala nundi dna ksheenata phalitamgaa shareera dravaalalo thymidine untundi. aneka antiviral aushadhaala charyaku thymidine kines avasaram. monoclonal pratirodhakaala utpattilo hybridoma kana rekhalanu enchukoovadaaniki idhi upayoginchabadutundi. clinical chemistrylo idhi pradhanamgaa hematological praanaantaka vyaadhula rooga nirdhaarana, chikitsa niyantrana mariyu phaalo-aplo vistarana markerga upayoginchabadutundi.
charitra.
dnalo thymidine kalayika 1950 loe pradarsinchabadindi. kontakaalam taruvaata, eee kalayikaku mundu phosphorylation anie choopabadindi. 
mariyu, 1960 loe, dheeniki kaaranamaina enjaim suddhi cheyabadindi mariyu vargeekarinchabadindi.
vargeekarana.
thymidine kinesla yokka rendu ververu taragatulu gurtinchabaddaayi mariyu eee suupar familylo cherchabaddaayih oka kutumba samuuhaalu herpes virus nundi thymidine kines alaage cellular thymidilate kineslanu kalipi, rendava kutumba samuuhaalu sakasherukaalu, bacteria, bacteria tii4, poxwiruslu, african swine feavour virus (asfv) mariyu fish lymphosystis disease virus (fldv) vanti vividha vanarula nundi vachayi. keetakaala indrajaala vairasla yokka pradhana capsid protein kooda eee kutumbaaniki chendinadi. procite namuunaa thymidine kinases yokka cellular rakanni matrame gurtistundi.
isozymes.
ksheeradaalaku rendu isoengimelu untaayi, avi rasaayanikamgaa chaalaa bhinnamgaa untaayi, tike1 mariyu tike2. modatidi modata pindam kanajaalamlo kanugonabadindi, rendavadi vayojana kanajaalamlo samruddhiga unnatlu kanugonabadindi, praarambhamlo vaatini pindam mariyu vayojana thymidine kines anie pilicheevaaru. tvaralo tk1 anedi kana vibhajana (kana chakram-aadhaarita) anchanaalo matrame cytoplasmlo undani choopabadindi, ayithe tk2 mitochondrialo undhi mariyu kana chakram-swatantramgaa untundi. rendu isoengimelu ververu praticharya gtsastranni kaligi untaayi mariyu ververu nirodhakaala dwara nirodhinchabadataayi.
viral thymidine kineslu ksheerada enjaimlaku nirmanatmakamga mariyu jeevarasaayanaparamgaa puurtigaa bhinnamgaa untaayi mariyu ksheerada enjaimlanu nirodhinchani inhibitors dwara nirodhinchabadataayi. rendu maanava isoengimela janyuvulu 1970la madhyalo sthaanikeekarinchabaddaayi. Tk1 kosam janyuvu clone cheyabadindi mariyu kramabaddheekarinchabadindi. sambandhita protein sumaru 25 kilo meterla paramaanu baruvunu kaligi untundi. saadhaaranamgaa, idhi sumaru 50 kilo meterla paramaanu baruvutho diamer gaa kanajaalamlo sambhavistundi. dheenini eah. tii. pii. dwara sakriyam cheyavachu. kriyaaseelakam chesina tarvaata, 100 kedi paramaanu baruvu kaligina tetramer. ayithe, prasaranalo unde enjaim roopam janyuvu dwara encode cheyabadina proteinku anugunamgaa undaduh prasaranalo kriyaaseela enjaim yokka pradhana bhaagam 730 kd paramaanu baruvunu kaligi untundi mariyu bahusha itara proteinlatho sanklishtamgaa bandhinchabadi untundi. eee sanklishtata marinta sthiramgaa untundi mariyu takkuva paramaanu baruvu roopaala kante ekkuva nirdishta kaaryaacharananu kaligi untundi.
recombinant tike1nii eee vidhamgaa sakriyam chesi tetramerga marchalemu, idhi kanaalalo sambhavinche enjaim samslaeshanha tarvaata savarinchabadindani choopistundi.
kana vibhajana yokka S dasalo tk1 anedi kanam dwara samslaeshanha cheyabadutundi. kana vibhajana puurtayina tarvaata, tk1 kanaantaramgaa ksheenistundi mariyu saadhaarana kana vibhajana tarvaata shareera dravaalaku velladu. kanamlo thymidine kines charyaku pratispandana-niyantrana undih thymidine trifasphate (ttp), thymidine yokka marinta phosphorylation yokka utpatti, thymidine kinesku nirodhakam vale panichestundi. idhi nucleic aamlam samslaeshanha kosam andubaatulo unna samatulya mottamlo titipini nirvahinchadaaniki upayogapadutundi, vyavasthanu atigaa santruptaparachadu. 5 '-aminothymidine, thymidine yokka vishapuuritam kaani analog, eee niyantrana yantraamgamlo jokyam chesukuntundi mariyu tadwara antinoplastic aushadhaalugaa upayoginche thymidine analogues yokka cytotoxicityni penchutundi. thymidine mariyu thymidine saaruupya phosphorylation yokka praticharya gatisastram sanklishtamainadi mariyu paakshikamgaa matrame telusu. thymidine trifasphate kuu thymidine yokka mottam phosphorylation mykhelis-menten kineticsnu anusarinchadu, mariyu thymidine mariyu euridine yokka vividha phosphatelu okadaanikokati phosphorylationku antaraayam kaligistaayi. vividha jaatula nundi tii. keey yokka gatisastram okadaanikokati bhinnamgaa untundi mariyu icchina jaatula nundi (monomer, diamer, tetramer mariyu seeram roopam) vividha roopaalu vibhina gathi lakshanaalanu kaligi untaayi.
herpes simplexes virus, varisella joster virus mariyu epstein-baar virus laloe virus nirdishta thymidine kinesla janyuvulu gurtinchabaddaayi.
 + eah. tii. pii --->
 + ADP
thymidine monophosphate mariyu eah. dii. pii. nii ivvadaaniki thymidine eah. tii. pii. thoo pratispandistundi.
function.
thymidine kinace dwara utprerakam cheyabadina praticharya yokka utpatti ayina thymidine monophosphate, thymidilate kinace enjaim dwara thymidine diphosphatega mariyu nucleoside diphosphate kinace enjaim dwara thymidine trifasphatega marintagaa phosphorylate cheyabadutundi. trifasphate oka dna anuvulo cherchabadutundi, idhi dna polymerase mariyu paripuurakaramaina dna anuvu (ledhaa riverse transcriptase vishayamlo rna anuvu, retroviruslo unde enjaim) dwara utprerakam cheyabadina praticharya.
thymidine monophosphate kooda dioxuridine monophosphate yokka methylation dwara vaerae praticharyalo kanam dwara utpatti cheyabadutundi, idhi thymidineku sambandham laeni itara jeevakriya maargaala utpatti, thymidilate synthase enjaim dwara. rendava maargam dna marammattu kosam thymidine monophosphate sarafara cheyadaaniki saripotundi. oka kanam vibhajinchadaaniki siddhamainappudu, DNA yokka puurti kotta amarika avasaram, mariyu thymidine triphosphate sahaa building blackla avasaram perugutundi. vibhajana samayamlo avasaramaina konni enjaimlanu tayaaru cheyadam dwara kanaalu kana vibhajanaku siddhamavutaayi. avi saadhaaranamgaa kanaalalo undavu mariyu taruvaata tagginchabadataayi mariyu digajaarutaayi. ituvanti enjaimlanu salvage enjaimlu antaaru. thymidine kines 1 atuvanti rakshana enjaim, ayithe thymidine kines 2 mariyu thymidilate synthase kana chakram-aadhaaritam kaadu.
lopam.
thymidine kines 2 nuu mitochondrial DNA samslaeshanha kosam kanaalu upayogistaayi. tike2 janyuvulo utparivartanalu mitochondrial dna depletion syndrome yokka mayopathik roopaaniki daariteestaayi. tike 2 lopaniki maroka kaaranam aakseekarana ottidi prerita ess-glutationation mariyu mitochondrial thymidine kines 2 yokka proteolytic ksheenata kaavacchu. tike 1 lopam valla kalige syndrome teliyadu, bahusha lopabhuishta tike 1 janyuvu pindam maranaaniki daariteestundi.
abhivruddhi samayamlo thymidine kines.
samslaeshanha tarvaata thymidine kines 1 yokka maarpu tarvaata tetramer erpadatam enjaim kaaryaacharananu penchutundi. enjaim karyakalapala niyantranaku idhi oka yantraangam anie soochinchabadindi. tetramerla nirmaanam dictiostelium abhivruddhi dasha tarvaata gamaninchabadutundi. dna samslaeshanha yokka chakkati niyantrana kosam daani upayogam vecchani raktamtho unna jantuvulu sakasherukaala nundi bayataku vacchina tarvaata sthaapinchabadindani soochinchabadindi. abhivruddhilo thymidine kines vanti enjaimla abhivruddhi kooda adhyayanam cheyabadindi.
jaatula pampinhee.
thymidine kines jantuvulu, mokkalu, konni bacteria, archaeans mariyu virus laloe untundi. poxes vairaslu, african swine feavour virus, herpes simplexes virus, varisella joster virus mariyu epstein-baar virus nundi thymidine kineslu gurtinchabaddaayi mariyu vividha sthaayilalo vargeekarinchabaddaayi. virus looni enjaim roopam itara jeevula kante bhinnamgaa untundi. sileendhraalalo thymidine kines undadu.
applications.
vibhajinche kanaala gurtimpu.
jeevarasaayana parisoedhanaloo thymidine kines yokka modati paroksha upayogam radiolabeld thymidine nuu cherchadam dwara kanaalanu vibhajinchadam mariyu vibhajana kanaalanu gurtinchadaaniki rediyodhaarmikata ledhaa autoradiography yokka tadupari kolata. eee prayojanam kosam tritated thymidine perugudala maadhyamamlo cherchabadutundi. saanketikatalo lopaalu unnappatikee, idhi ippatikee praanaantaka kanaala perugudala retunu nirnayinchadaaniki mariyu roganirodhaka saastramlo lymphocytela kriyaaseelatanu adhyayanam cheyadaaniki upayoginchabadutundi.
kriyaaseela kanitula pempudu jantuvula skaan.
fluorothymidine anedi thymidine analog. daani teesukovadam thymidine kines 1 dwara niyantrinchabadutundi, anduvalla idhi vaegamgaa kaniti kanajaalaanni vistarinchadam dwara praadhaanyatanistundi. florin isotope 18 anedi positron emission tomography (pempudu jantuvu) loe upayoginche positron emiter. anduvalla fluorin-18 radiolabel cheyabadina fluorothymidine eff-18 kriyaaseela kaniti vistarana yokka pempudu jantuvula imagingku upayogapadutundi mariyu saadhaaranamgaa upayoginche marker fluedoxiglucose (18eff) thoo anukuulamgaa polchabadutundi. clinical adhyayanalanu poolchadaaniki sahayapade praamaanika protocol soochinchabadindi.
hybridola empika.
hybridomas anevi kaniti kanaalanu (ivi anantamgaa vibhajinchagalavu) mariyu immunoglobulin utpatti chese lymphocytelanu (plasma kanaalu) kalapadam dwara pondina kanaalu. icchina pratyaekamaina vishishtata (monoclonal antibodies) thoo pedda mottamlo immunoglobulinlanu utpatti cheyadaaniki hybridolanu vistarinchavacchu. oka samasya emitante, kana kalayika tarvaata upayoginchani kanaala yokka adhika adanapu nundi hybridomalanu vaeru cheyadam. dheenini parishkarinchadaaniki oka saadhaarana maargam fusion kosam thymidine kines negative (tk) tumour sell linelanu upayoginchadam. thymidine kines negative kanaalu thymidine analogues samakshamlo kaniti kana rekhanu penchadam dwara pondabadataayi, ivi thymidine kines positive (tike +) kanaalanu champutayi. appudu pratikuula kanaalanu vistarinchavacchu mariyu tike + plasma kanaalatoe kalayika kosam upayoginchavacchu. kalayika taruvaata, kanaalu methotrexate ledhaa aminopterine thoo oka maadhyamamlo perugutaayi, ivi enjaim dihydropholate reductasenu nirodhistaayi, tadwara thymidine monophosphate yokka dii novo samshlaeshanhanu nirodhistaayi. saadhaaranamgaa upayoginche atuvanti madhyamam topi madhyamam, indulo hypoxanthine, aminopterine mariyu thymidine untaayi. thymidine kines-lopam unna kana rekha nundi upayoginchani kanaalu thymidine monophosphate muulam lenanduna chanipotayi. lymphocytelu chivariki maranistaayi endukante avi "amaramainavi" kaavu."vaari kana rekha puurveekula nundi" "amaratvam" "kaligi unna hybridomas mariyu plasma kanam nundi thymidine kines matrame manugada saagistaayi". kaavalasina pratirakshakaalanu utpatti chese vaatini enchukuni, monoclonal pratirakshakaalanu utpatti cheyadaaniki samskaristaaru. hybridoma kanaalanu maroka janyuvu ayina hgprtki sambandhinchi vivarinchina adhe suutraanni upayoginchi kooda vaeru cheyavachu, idhi rakshana maargamlo gmp nucleotide samshlaeshanhaku avasaramaina impnu samslaeshanha chestundi.
chromosome nirmaanam yokka adhyayanam.
dna fibers yokka molecular duvvenanu budding eest saccaromysis serevisialo chromosomela nirmaanaanni paryaveekshinchadaaniki upayoginchavacchu. idhi vyaktigata anuvula dna replication profilenu andistundi. dheeniki eest jaatulu thymidine kinesnu vyaktam cheyalsina avasaram undhi, idhi adavi rakam eest kaadu, idhi sileendhram (sambhavinche sanghatananu chudandi). anduvalla, thymidine kines kosam janyuvunu janyuvulo chaerchaali.
clinical chemistry.
thymidine kines anedi oka rakshana enjaim, idhi kana vibajananu oohinchi matrame untundi. kana vibhajana tarvaata avasaram laeni proteinlanu ksheenimpajeyadaaniki kanaalaku pratyeka yantraangam unna saadhaarana vibhajanaku guraina kanaala nundi enjaim vidudala cheyabadadu. saadhaarana vishayaalalo, seeram ledhaa plasmalo thymidine kines mottam chaalaa takkuvagaa untundi. kaniti kanaalu bahusha chanipoyina ledhaa maranistunna kaniti kanaala antaraayam kaliginchadaaniki sambandhinchi, prasaranaku enjaimnu vidudala chestaayi. anduvalla seeramlo thymidine kines sthaayi kaniti yokka dookudu kolatagaa parokshamgaa praanaantaka vistarana kolatagaa panichestundi.
chikitsa anuvartanaalu.
konni mandulu pratyaekamgaa kanaalanu vibhajinchadaaniki vyatiraekamgaa nirdesinchabadataayi. vyaadhigrasta kanaalu saadhaarana kanaala kante chaalaa tarachugaa pratiroopam chestaayi mariyu athi vegavantamaina kana pratiruupanaku sambandhinchina konni praanaantaka vyaadhulaku kooda vyatiraekamgaa (udaaharanaku psoriasis), vaatini kanitulu mariyu viral vyaadhulaku (retrovirus mariyu itara vairasku vyatiraekamgaa) vyatiraekamgaa upayoginchavacchu. thymidine analogues yokka antiviral mariyu cancer nirodhaka charya, kaneesam paakshikamgaa, mitochondrial thymidine kines yokka down-regulation dwara saadhinchabadutundani soochinchabadindi.
cytostatics.
thymidine jeevakriyaku vyatiraekamgaa vividha rakaala mandulu unnaayi, tadwara cancer sambandhita kana vibajananu niyantrinchadaaniki upayoginche thymidine kines untundi. golusu terminatorlu anevi thymidine analaaglu, ivi perugutunna dna golusulo chaerchabadataayi, conny golusunu marinta podiginchaleni vidhamgaa savarinchabadataayi. thymidine yokka saaruupyaalugaa, eee rakamaina mandulu takshaname 5 '-monophosphatelaku phosphorylate cheyabadataayi. monophosphate marinta sambandhita trifasphate kuu phosphorylate cheyabadutundi mariyu perugutunna dna golusulo cherchabadutundi. analog savarinchabadindi, tadwara idhi nirantara golusu perugudalaku avasaramaina 3 '-sthithilo hydroxyl samuuhaanni kaligi undadu. gdovudin (azt; atc: j05af01) loe 3 '-hydroxyl samuuham azido samuuham dwara bharti cheyabadindi, stavudine (atc: j05af04) loe idhi bharti cheyakunda tolaginchabadindi. seeramlo thymidine kinesnu nirnayinche paddhatullo okadaanilo ajtnu uparitalamgaa upayogistaaru. idhi ajt eee paddhatiloo jokyam chesukuntundani mariyu oka parimiti kaavacchu anie suchistundihi hechaivi infectionlo haart therapylo ajt oka praamaanika bhaagam. aids yokka oka saadhaarana paryavasanamga lymphoma mariyu thymidine kines nirdhaarana yokka atyanta mukhyamaina roganirdhaarana anuvartanam lymphoma paryaveekshanha kosam.
itara thymidine saaruupyaalu, udaaharanaku idoxuridine (etisi zhe05ebi02) tadupari replication chakraalalo bays jatanu nirodhinchadam dwara panichestundi, tadwara phalitamgaa dna golusu lopabhooyishtamgaa untundi. praanaantaka kanaala apoptosis saadhinchadaaniki dheenini rediyodhaarmikatato kooda kalapavacchu.
antivirals.
konni antiviral mandulu, asaiklovir (etisi zhe05ebi01) mariyu gunsiclovir (etisi zhe05ebi06) alaage itara nucleoside analogues vantivi maanava thymidine kineslaku viruddhamgaa viral thymidine kines yokka uparitala nirdishtatanu upayogistaayi. eee mandulu anukuula aushadhaalugaa panicheestaayi, ivi vishapuuritamainavi kaavu, conny viral thymidine kines dwara phosphorylation dwara vishapuurita aushadhaalugaa maarchabadataayi. virus sokina kanaalu anduvalla atyanta vishapuurita trifasphatelanu utpatti chestaayi, ivi kana maranaaniki daariteestaayi. dheeniki viruddhamgaa, maanava thymidine kines, daani marinta irukaina vishishtathatho, phosphorylate cheyalemu mariyu prodragnu sakriyam cheyalemu. eee vidhamgaa, virus baarina padina kanaalu matrame aushadhaaniki gurayye avakaasam untundi. ituvanti mandulu herpes samuuham nundi vaati nirdishta thymidine kinesto vairaslaku vyatiraekamgaa matrame prabhaavavantamgaa untaayi. eee rakamaina mandulatho chikitsa pondina rogulalo, antiviral aushadha nirodhakata abhivruddhi tarachugaa gamaninchabadutundi. herpes simplexes virus mariyu varisella joster virus loe thymidine kines janyuvunu kramabaddhikarinchadam vegavantamaina janyu vaividhyaanni chooputundi mariyu antiviral aushadha nirodhakata nirdhaarananu sulabhataram chestundi.
1979 decemberulo mashoochi nirmoolinchabadindani prakatinchina taruvaata, teaca karyakramalu nilipiveyabaddaayi. pramaadavasaattu ledhaa jeeva yuddham phalitamgaa vyaadhi tirigi udbhavinchadam asurakshita janabhanu kalustundi mariyu niyantrinchadam kashtamgaa unde mahammariki daariteeyavachchu. mashoochi mahammarini edurkovadaniki saamuuhika teekaalu veyadam savaluga untundi, endukante aamodinchabadina mashoochi teaca, vaccinia virus, teevramaina dushprabhaavaalanu kaligi untundi. ayinappatikee, konni prabhutvaalu mashoochi teekaanu nilva chestaayi, eee avakaasam nundi rakshana kalpinchadaaniki. ayithe, nirdishta mariyu samarthavanthamaina antiviral aushadhaala abhivruddhiki praadhaanyata ivvabadutundi. herpes vairasku vyatiraekamgaa mandula kosam upayoginche vidhamgaane, eee prayojanam kosam poxwirus yokka thymidine kines yokka pratyaekatanu upayoginchadam anedi oka saadhyamayye vidhanam. oka ibbandi emitante, poxwirus thymidine kines maanava thymidine kinesla maadirigaane thymidine kinesla kutumbaaniki chendinadi mariyu tadwara rasaayanikamgaa marinta saaruupyata kaligi untundi. anduvalla sambhaavya antiviral aushadhaalanu kanugonadaaniki poxwirus thymidine kinesla nirmaanam nirnayinchabadindi. ayithe, eee shodhanalo paxviruslaku vyatiraekamgaa upayoginchagala antiviral aushadham inca dorakaledu.
janyu chikitsalo "aatmahatya janyuvu" gaa.
herpes virus thymidine kines janyuvu janyu chikitsa prayogaalalo bhadrata vyavasthagaa "aatmahatya janyuvu" gaa kooda upayoginchabadindi, idhi janyuvunu vyakteekarinchae kanaalanu gunsiclovir upayoginchi champadaaniki veelu kalpistundi. recombinant janyuvu aniyantrita kana perugudalaku (insertional mutagenesis) daariteese utparivartanaku kaaranamaite idhi manchidi. eee savarinchina kanaala dwara utpatti cheyabadina cytotoxic utpattulu porugu kanaalaku vyaapinchi, vaatini gansiklovirku samanamga avakaasam kalpistaayi, eee drugvishayaanni "baistaandar effect" anie pilustaaru."eee vidhanam janthu namoonaalalo cancerku chikitsa cheyadaaniki upayoginchabadindi, mariyu janyuvunu vyaktam chese praanaantaka kanaalalo 10 saatam varaku kanitini champadam valla idhi prayojanakaramgaa untundi. tomato thymidine kines mariyu ajt upayoginchi idhe vidhamaina vyavasthanu prayatninchaaru. adanamgaa, thymidine kines janyuvunu 2016loe airopalo sharatulatho aamodinchabadina gelmaxis anae hematopoietic stem sell transplant therapylo pramaadakaramaina graft-verses host vyaadhini parishkarinchadaaniki aatmahatya janyuvugaa upayogistaaru.
kaniti marker janyuvulu.
thymidine kines yokka idhe vidhamaina upayogam saadhaarana kanaalalo (kaniti gurtulu) laeni padaarthaala yokka konni kaniti kanaalalo unikini upayogistundi. atuvanti kaniti gurtulu, udaaharanaku, cea (carcinoembrionic antigen) mariyu afp (alfa phetoprotein). eee kaniti gurtula janyuvulanu thymidine kines kosam prothsaahaka janyuvulugaa upayoginchavacchu. thymidine kines appudu kaniti gurtunu vyaktam chese kanaalalo sakriyam cheyabadutundi, conny saadhaarana kanaalalo kaadu, antey gansiklovirtho chikitsa kaniti kanaalanu matrame champutundi. kaniti kanaalaku janyu badileeni lakshyamgaa chesukovadamto sambandham unna samasyalu inca puurtigaa parishkarinchabadaledu kabatti, ituvanti janyu chikitsa-aadhaarita vidhanalu ippatikee prayogaatmakamgaa unnaayi.
kanitulaku neutron capture therapy.
medadu kanitula boran neutron capture therapy kosam boranto thymidine analaagnu chaerchaalani soochinchabadindi mariyu janthu namoonaalalo prayatninchabadindi. boran kaligi unna thymidine utpannaalu chaalaa vistrutamaina sankhyalo vivarinchabaddaayi.
antiparasitis.
paraannajeevi janyuvulo oka tii. keey. janyuvunu pravesapettadam valla bradunu cherchadam saadhyamavutundi, tadwara eee aushadhaanni upayoginchi chikitsaku paraannajeevi sunnitamgaa maarutundani kooda soochinchabadindi mariyu paraannajeevi janyuvu yokka pratiruupanaku sunnitamaina suuchikagaa untundi.
kolata.
seeram mariyu plasma loe.
seeram ledhaa plasmalo thymidine kines sthaayilu ekkuvagaa enjaim activity asses lanu upayoginchi kolustaaru. vaanijyaparamaina madimpulalo, idhi uparitala saaruupyatatoe seeram namuunaanu podiginchadam dwara mariyu utpatti parimaanaanni kolavadam dwara jarugutundi. immunoasse dwara thymidine kines protein yokka pratyaksha nirdhaarana kooda upayoginchabadindi. eee paddhati dwara kanugonabadina thymidine kines parimaanaalu enjaim kaaryakalaapaalato baga sambandham kaligi undavu. dheeniki oka kaaranam emitante, immunoasse dwara gurtinchabadina pedda mottamlo seeram tike1 enjaimga churukuga undadu. idhi mukhyamgaa roganirodhaka saktini marinta sunnitamgaa unde ghana kanitula vishayamlo jarugutundi.
kanajaalamlo.
kanajaalam velikiteesina tarvaata kanajaala namoonaalalo thymidine kines nirnayinchabadindi. velikiteeta ledhaa vislaeshanha kosam praamaanika paddhati abhivruddhi cheyabadaledu mariyu kanaalu mariyu kanajaalaala nundi padaardhaalalo nirnayinchadam anedi yea nirdishta clinical prasnaku sambandhinchi dhruvikarinchabadaledu, ayithe chudandi roman mariyu itarulu. mariyu arner mariyu itarulu. uparitala analog 5-bromovinyl 2 '-deoxuridine upayoginchi kana saralalo tike2 yokka nirdishta nirdhaarana kosam oka paddhati abhivruddhi cheyabadindi. krinda perkonna adhyayanaalalo upayoginchina paddhatulu mariyu phalitaalu nivedinchabadina vidhanam chaalaa bhinnamgaa untaayi, vividha adhyayanala madhya poolikalu saadhyam kaavu. abhivruddhi samayamlo pindam kanajaalaalo tii. keey. 1 sthaayilu taruvaata sambandhita kanajaalaala kante ekkuvagaa untaayi. konni praanaantaka vyaadhulu kanaalu mariyu kanajaalamlo tii. keey. viluvala naatakeeya perugudalaku kooda daariteestaayih monocytosis samayamlo paridheeya lymphocytelalo mariyu haanikaramaina raktaheenata samayamlo emuka majjalo. kana vibhajana samayamlo kanaalalo tike1 unnanduna, praanaantaka kanajaalamlo tike charya sambandhita saadhaarana kanajaalam kante ekkuvagaa undaalani anukovadam sahetukamainadi. chaalaa adhyayanaalalo kooda idhi nirdhaarinchabadindi.
immunohystochemical steining.
immunohystochemical detection kosam thymidine kinesku vyatiraekamgaa pratiroedhakaalu andubaatulo unnaayi. dasha 2 rommu cancer unna rogulanu gurtinchadaaniki thymidine kines kosam marakalu veyadam nammadagina saanketikatagaa kanugonabadindi. thymidine kines mariyu kii-67 maraka kalayika dwara atyadhika sankhyalo rogulanu gurtinchaaru. oopiritittula cancer, colorectal carcinoma, oopiritittula cancer mariyu moothrapindaala kana cancer kosam kooda eee saanketikata dhruvikarinchabadindi.
fluorescent steining.
2 '-dioxy-2', 2 '-diaphloro-5-ethinyluridine (df-edu) herpes simplexes virus thymidine kinesto bandhistundi, conny, sterical addanki kaaranamgaa, maanava thymidine kinesto kaadu. eee karakamu fluorescent ajaidto kalisi sokina kanaala fluorescensenu kaligistundi, conny sokani kanaalaki kaadu. anduvalla, eee uparitala analog vyaadhi sokina kanaalanu pratyaekamgaa maraka vaeyadaaniki veelu kalpistundi.

