
ativyaapti maccha

supravital steining anedi oka jeevi nundi tolaginchabadina jeeva kanaalanu pariseelinchadaaniki suukshmadarshini loe upayoginche steining paddhati. idhi sareeramloki marakanu inject cheyadam ledhaa pravesapettadam dwara chese intravital steining nundi bhinnamgaa untundi. anduvalla oka ativyaapti maraka ekkuva vishapuuritatanu kaligi undavacchu, endukante konni kanaalu matrame daanini kontakaalam manugada saaginchaalsina avasaram undhi. keelaka maraka" anae padaanni kontamandi rachayitalu pratyaekamgaa intravital marakanu suuchimchadaaniki upayogistaaru, mariyu itarulu parasparam oka supravital marakatho marchukuntaru, pradhana bhavana emitante pareekshinchabadutunna kanam ippatikee sajeevamgaa undhi. kanaalu sajeevamgaa mariyu sthiramgaa lenanduna, sareeram velupala, ativyaapti marakalu taatkaalika swabhaavam kaligi untaayi.
atyanta saadhaaranamaina supravital steyn kotta methylene neelam ledhaa adbhutamaina cressil neelam rangunu upayoginchi reticulocytelapy nirvahinchabadutundi, idhi eee sajeeva aparipakva erra rakta kanaalalo supravital steynla dwara vilakshanamgaa avakshepinchabadina ribozomela reticulophilamentus namuunaanu chuudataaniki veelu kalpistundi. atuvanti kanaala sankhyanu lekkinchadam dwara erra rakta kanaala erpade retunu nirnayinchavacchu, idhi emuka majja karyakalapalu mariyu raktaheenatapai antardrushtini andistundi. idhi keelaka marakku viruddhamgaa untundi, upayoginchina rangu sajeeva kanaala nundi "minahaayinchabadina" rangugaa unnappudu, tadwara chanipoyina kanaalu matrame sanukoolamgaa marakalu padataayi. (keelaka marakalu tripan blue mariyu propedium iodide vanti rangulu, ivi chaalaa sthuulamgaa untaayi ledhaa kana poranu daatadaaniki chaalaa chaarj cheyabadataayi ledhaa sajeeva kanaala dwara churukuga vaegamgaa bayataku pampabadataayi.)
mukha vislaeshanha vanti anuvartanaala kosam kana uparitala antibody steining (immunofluorescence) thoo supravital steiningnu kalapavacchu. detergent triton x-100 nuu upayoginchi reversible sell paaragamyata dwara sajeeva kanaala lopali bhaagamlo immunofluorescence kooda cheyavachu. kanaala sankhyaku tagina yekagrataku jagrattagaa sardubaatu cheyabadi, prii treatment 1 nundi 150 kilodaltonla madhya anuvulanu kana lopali bhaagaaniki cherukovadaniki anumatistundi. eee sandarbhamlo pratiroedhakaalanu idhe vidhamgaa upayoginchinappatiki, "supravital steyn" anae padam saadhaaranamgaa antargatamgaa tagina lakshanaalanu kaligi unna chinna rasaayanaala kosam ketayinchabadutundi.
saadhaarana ativyaapti rangula udaaharanalu.
aarr. bii. sii. la kosam ativyaapti rangulu