
ribonucles ii

ribonuclease ii anedi oka bacteria ribonuclease, idhi ribosomal rna (9ess nundi 5ess rna) yokka prosessing mariyu bulk cellular rna yokka rasayana ksheenatalo paalgontundi.
cellular sthaanikeekarana.
ribozomelu mariyu mudi poralu avakshepanalu avadamtho, arnes ii anedi kana pora protein complexelo bhaagamgaa soochinchabadindi. suukshmadarshini antargata cytoplasmic poraku ledhaa lopali poratho daggari sambandham unna oka helical cytoskelital nirmaanaaniki tagg cheyabadina rnase e nii sthaanikeekarinchindi.
protein nirmaanam.
eee enjaim 1,061 avasheshaalanu kaligi untundi mariyu rendu vibhina kriyaatmaka praantalugaa vidipotundi, ivi 5 'n-terminus vadda unna pedda domine mariyu 3' c-terminus vadda unna chinna domine. enn-terminal sagam utpreraka domaine yerparustundi, sii-terminal sagam degradozome paranjaanu yerparustundi. loha-bandhinche paaket vaatini rnase e protein nirmaanam madhyalo vaeru chestundi. ayithe degradozome erpadatam anedi e kii keelaka paatra pooshinchadu. kolai perugudala, sii-terminal sagam tolagimpu konni arnes ii uparitalaalu ksheeninche retunu taggistundani kanugonabadindi.
ribonucles ii anedi tetrameric conformationlo panichestundi, idhi handle praantamloo anusandhaaninchabadina rendu kattera laaga kanipinche nirmaanaanni roopondinchadaaniki okadaanitoe okati anubandhinchabadina naalugu upabhaagaalanu kaligi untundi. kattera blade pedda domaintho tayaaru cheyabadindi mariyu handle chinna domaintho tayaaru cheyabadindi. pedda domine yokka utpreraka pradaesamloe, rnase h sub domine, dnase i sub domine, s1 sub domine mariyu 5 'sensing region vanti naalugu sub domainlu unnaayi. eee naalugu upabhaagaalu vaati paniteeru mariyu sajatiya nirmaana madatalu saaruupyata aadhaaramgaa vibhajinchabaddaayi. rnase h anedi n-terminal praarambhamlo undhi mariyu rnase h endoribonuclese kutumbam paeru pettabadindi, endukante avi okelanti nirmaanaanni panchukuntaayi; ayithe, rnase h utpreraka vidhiga kakunda nirmaanaatmaka vidhiga panichestundi endukante idhi kriyaaseela-sait avasheshaalu lekapovadam. taruvaata, s1 sub domine mariyu 5 'sensing praantam rnase h fold loe amarchabadataayi. rnase e s1 domine oka obi-foldnu sweekaristundi, dheenilo soukaryavantamaina looplu baga arder cheyabadina aidu-stranded β-barrell korku jodinchabadataayi. ribonuclease eloe, s1 domine dimerisation dwara tetrameric quaterinary nirmaanam erpadataaniki sahayapadatame kakunda, eee tetrameric enjaimloni utpreraka domainla dwara rna jalavisleshananu sulabhataram cheyadaaniki uparitala-binding saitga kooda panichestundi. ess1 sub domaintho, 5 'sensing praantam uparitala-binding saitga panichestundi, idhi oka upabhaagamlo lakshya rna anuvunu sthireekarinchadaaniki sahaayapadutundi, tadwara diamerloni itara upabhaagaalu aasakti unna rnanu cheelchagalavu. utpreraka pradaesaaniki dhooramlo unna 5 'sensing praantam, idhi dnase i upa domainlo untundi. rnase e utpreraka sait yokka chivari upa domine dnase i, idhi double-stranded dna nuu cheelche endonuclease nirmaanamtho daani conformational saaruupyataku paeru pettabadindi. ribonucles e loe, diamer interfacelo aadhipatyam chelaayinchadaaniki dnase i sub domine sweeya-puurti chestundi. alaage, rna vennemuka yokka jalavisleshita daadi dwara cheelikaku madhyavartitvam vahinche rendu magnitium ayan binding sait mariyu rendu upa unitlatho chesina diamer sthireekaranaku sahayapade rendu jink ayan binding sait kooda unnaayi.
function.
"escherichia kooli" endoribonuclese ii janyu vyakteekaranapai gananeeyamaina prabhavanni chooputundi. idhi ribosomal rna (rrna) mariyu badilee rna (trna) paripakvataku matrame kakunda jalavisleshana praticharya dwara sandesavaahaka rna (mrna) yokka vegavantamaina ksheenataku kooda avasaram. 
rrna puurvagaami paripakvatalo, prosessing kosam uparitalaalu nagna rnalu kaavu, conny kontavaraku asampoornamaina, savarinchani prii-rrna-ribosomal protein complexelu. prii-16luu mariyu prii-23luu rendintinee praathamika rna-protein complexes nundi arnes III dwara tolagistaaru, idhi 5 'monophosphorylated cheelika utpattulanu utpatti cheyadam dwara rna paripakvatalo tadupari dasalanu sakriyam chestundi. rnase e anedi 16s rrna yokka 17la puurvagaamini marinta taggistundi. eee charya rnase g dwara rrna yokka 5 'paripakvatanu sulabhataram cheyadaaniki mariyu excise prii-5s rrna kii rendu cheelikalu cheyadaaniki sahaayapadutundi. tranala vishayamlo, 86 tranala jaatulalo sumaru 50 ii. koliki rnase e avasaram. ribonucles ii anedi tranala yokka 3 'chivaralo trananu kaligi unna praathamika transcriptsnu cheelchivestundi. eee cheelikalu vyaktigata trana puurvagaamulanu vaeru cheyadaaniki mariyu tranalanu mrna ledhaa terminator sequencela nundi vaeru cheyadaaniki upayogapadataayi. ribonuclease ii yokka praadhamika pani 3 'exonucleasela dwara accessnu praarambhinchadaaniki parinati chendina 3' chivarana unna pradaesamloe cheeladam.
mrna ksheenatalo, ribonucles ii anedi eah-mariyu yuu-sampanna praantaalalo single-stranded rnanu gurtinchi vidadeestundi. rnase e utpreraka domine empikagaa 5′-monophosphate rna terminitho bandhistundi, conny 3′ nundi 5′ disalo cheelika modnu kaligi untundi. rnase e anedi 3 'nundi 5' scanning mechanism dwara cheelika pradaesaalanu gurtinchagaladu. eee uparitalaala yokka 5′-monophosphorylated chivarana rnase e yokka anker prosessive modlo sambhavinche directional cheelikala kosam enjaimnu orient chestundi. rna lenappudu, arnes eee yokka ess1 sub domine mariyu 5 'sensing sait rendoo chuttupakkala draavakanki bahirgatamavutaayi, idhi rnani takshaname bandhinchadaaniki veelu kalpistundi. rna samakshamlo, target rna open configurationlo samyukta ess1 sub domine mariyu 5′ sensaartho bandhistundi. rna pradhanamgaa 5′ sensor yokka binding anubandham dwara langaru veyabadutundi mariyu ess1 sub domainloni hydrophobic uparitala pyaach dwara aadhaaritam. S1 sub domine anuvunu orient cheyadaaniki panicheestundagaa, 5 'sensing paaket uparitala-binding anubandhaanni gananeeyamgaa dohadam chestundi. eee rendu saitlu rnanu kaligi undagaa, fusion 5/ess1 sub domine closed configurationloki oke complexga kadulutundi. idhi uparitalaanni utpreraka pradaesaaniki sameepamlo teesukuvastundi, ikkada hydroxyl samuuham nucleophilic daadi praticharya dwara fasfate vennemukapai daadi chestundi. eee pratispandana magnitium ayan dwara madhyavartitvam cheyabadutundi. aasaktigala rna cheelipoyinappudu, mariyu rnase e open configurationku tirigi vachinappudu praticharya yokka utpattulu chivariki vidudala cheyabadataayi. antekakunda, rnase e nii sweeya-niyantrinchavacchu, dheeni dwara ribonuclease e yokka mrna mottam cellular rnase e kaaryaacharanaku sensorga panichestundi mariyu tadwara uparitalaalu labhyata mariyu vruddhi raetu maarpula kaaranamgaa rnase e kaaryaacharananu parimitam chestundi.
eee yokka ribonucles eee poolika. kolai mariyu itara jeevulu.
escherichia kooli" yokka ribonucles ii kii sambandhinchina vividha bacteria shrenula amarika aadhaaramgaa, daadaapu 70 saatam shrenulu shrenula praarambhamlo baga samrakshinchabadataayi mariyu shrenula chivarilo sarigga samrakshinchabadavu. aidu itara jeevula shrenulanu ribonucles e yokka shrenitho polchinappudu, ribonucles e/g kutumbaaniki chendina sabhyudu oke hydrolyse functionnu kaligi unnanduna chaalaa shrenulu n-terminal vadda oke avasheshaalanu panchukunnatlu kanipistundi. maroe maatalo cheppalante, ribonucles ii/zii kutumba sabhyudu yokka pedda utpreraka domine daadaapu okate. dheeniki viruddhamgaa, sii-terminus vadda unna chinna nirmaanaatmaka domine, vividha jeevulaku bhinnamgaa untundi, endukante chinna domine itara enjaimlaku paranjaga panichese nirmaana kramaanni kaligi untundi. udaaharanaku, "sedicia davise" loe unna ribonucles ii anedi s3jyp0 janyuvu nundi vachindi. sedesia davise" loe ribonuclease ii yokka nirmaanaanni gamaninchinappudu, utpreraka domine kramamlo 31-119 avasheshaala vadda unchabadina s1 muulaamsaanni mariyu "escherichia kooli" yokka rnase e loe s1 domine mariyu metal-binding domine vale adhe sthaanamlo unna shrenulapai 404-407 avasheshaala vadda unchina loha-binding saitnu kaligi untundi.
parinaamaatmaka charitra.
pradhanamgaa rna gvakriyalo palgone ribonucleasis (arnes) protein kutumbam, rna paripakvata, rna end-turnover mariyu kanamlo asaadhaarana rna ledhaa gaduvu mugisina jaatula ksheenatalo mukhyamaina paatralu poeshistumdi. avi vaati adhokaranam karyakalapala aadhaaramgaa exoribonucleasis mariyu endoribonucleasizga vargeekarinchabaddaayi. ribonuclease e (rnase e) praarambhamlo "escherichia kooli" jaathi k12 nundi endoribonucleisga kanugonabadindi. idhi dna kramam vislaeshanha, e yokka orthologla aadhaaramgaa. dozenla koddi parinaamatmakamgaa vibhinnamaina bacteria jaatulalo kolai arnes ii unikilo undani anchanaa veyabadindi. ii loe. kolai, ribonucles ii enjaim chaalaa varaku mrnla kshayam vanti rna jeevakriyanu niyantrinchadam dwara kana saadhyatanu niyantrinchadamlo mukhyamaina paatra poeshistumdi mariyu prii-trnla prosessingnu sakriyam chestundi. adhokaranam functiontho paatu, 5s ribosomal rna, tranas mariyu arnes pii ribozyme yokka emm1 rna bhaagam yokka puurvagaamula paripakvataku arnes ii avasaram.