
phospholipase A1

phospholipase a1 (ec 3.1.1.32; kramabaddhamaina paeruh phosphatidilcoline 1-asylhydrolase) anedi plaa1eah janyuvu dwara encode cheyabadina oka phospholipase enjaim, idhi 1-asil samuuhaanni tolagistundihi 
idhi phospholipase anie piluvabade enjaimla taragatilo nivasinche enjaim, idhi phospholipidlanu kovvu aamlalugaa hydrolyse chestundi. avi utprerakam chese praticharya rakanni batti vaeru cheyabadina naalugu taragatulu unnaayi. mukhyamgaa, phospholipase a1 (pla1) pratyaekamgaa phospholipids yokka "sn"-1 sthaanam vadda cheelikanu utprerakam chestundi, kovvu aamlam mariyu lysophospholipidnu yerparustundi.
function.
plaa1luu maanavulato sahaa aneka jaatulalo unnaayi, mariyu lysophospholipid madhyavartula utpattini niyantrinchadam mariyu sulabhataram cheyadam mariyu jeerna enjaimlugaa panicheyadam vanti vividha rakaala cellular vidhulanu kaligi untaayi. eee enjaimlu cellular phospholipids yokka vegavantamaina turnover retlaku baadhyata vahistaayi. dheeniki adanamgaa, kovvu aamlam mariyu lysophospholipid ayina plaa1 dwara utprerakam cheyabadina praticharya yokka utpattulu platelet aggregation mariyu mruduvaina kandaraala sankocham vanti vividha jeevasambandhamaina vidhulalo mukhyamainavi. adanamgaa, lysophospholipidsnu aahara paddhatulu mariyu soundarya sadhanalalo sarfaktaantlugaa chudavachu mariyu aushadha pampinheelo upayoginchavacchu. plaa1 aneka jaatulalo kanipistunnanduna, adhyayanam cheyabadutunna jeevi aadhaaramgaa eee oka nirdishta enjaimlo vividha taragatulu unnayani kanugonabadindi.
jaatulu mariyu kanajaala pampinhee.
plaa1loe aneka vaividhyaalu unnaayi, idhi unna prati jeevi madhya koddigaa bhinnamgaa untundi. mukhyamgaa, idhi eluka kaaleyam mariyu bovin medadula plasma vanti ksheerada kanaalalo kanipistundi, mariyu metazone paraannajeevulu, protojova paraannajeevulu mariyu paamu vishamlo kooda kanipistundi.
uparitala pratyekata.
plaa1 ionic uparitalaala kante ionic kaani uparitalaalanu pradhaanyamgaa jalavisleshanam chestundi. tatastha phospholipidlapy pla1 charyaku vaanchaneeya pihech paristhitulu sumaru 7.5 kaga, aamla phospholipidlapy pla1 charyaku vaanchaneeya paristhitulu sumaru 4.
nirmaanam.
plaa1 yokka nirmaanam eee krindi kramaanni kaligi unna monomer gly-x-serr-x-gly, ikkada x edainaa itara amaino aamlanni suuchistumdi. serin enjaimlo kriyaaseela pradaesamgaa pariganinchabadutundi. plaa1laloe disulfide bandham erpadataaniki avasaramaina vividha rakaala systeine avasheshaalatho serr-asp-hiss yokka utpreraka trayam kooda untundi. systeine avasheshaalu mootha domine mariyu bii9 domine vanti keelaka nirmaanaatmaka muulaamsaalaku baadhyata vahistaayi, eee rendoo lipid binding uparitala looplu. eee rendu looplu prati plaa1 madhya maaravacchu. udaaharanaku, podavaina mootha domine (22-23 amaino aamlalu) mariyu podavaina bii9 domine (18-19 amaino aamlalu) kaligina plaa1 enjaim tricylglycerol hydroless kaaryaacharananu pradarsinche extra cellular plaa1 nuu kaligi untundi. dheeniki viruddhamgaa, marinta empikagaa pariganinchabade oka pla1 enjaim varusaga 7-12 mariyu 12-13 amaino aamlalalo vistarinchi unna chinna mootha mariyu bii9 domine kaligi untundi.
paarisraamika viniyogam.
plaa2 vanti itara phospholipasela maadirigaa kakunda, eee enjaimnu suddhi cheyadaaniki, clone cheyadaaniki, vyakteekarinchadaaniki mariyu vargeekarinchadaaniki samarthavanthamaina maargam lekapovadam valla plaa1 gurinchi chaalaa teliyadu. dheeni kaaranamgaa pla1 prastutam vaanijyaparamgaa andubaatulo ledhu. lysophospholipidsnu aahara paddhatulu mariyu soundarya sadhanalalo sarfaktaantlugaa chudavachu mariyu aushadha pampinheelo upayoginchavacchu. pla1 utpattiki tagina vruddhi vaataavaranaalanu nirnayinchadaaniki prastuta parisoedhanalu amalu cheyabadutunnayi. oka nirdishta adhyayanamlo, plaa1 nuu ess dwara utpatti cheyavachani kanugonabadindi. serivisia "mariyu" eah. oriza ". eee pla1 utpatti samskrutulalo, natrajani mariyu carbon vanarulanu penchadam pla1 digubadi perugudalaku daariteestundi.
aavishkarana.
1900la praarambhamlo, phosphatidylcoline thoo pancreatic rasaanni podigina tarvaata uchita kovvu aamlalu paerukupoetunnaayani chuupistuu oka pariseelana jarigindi. gamaninchina plaa1 karyakalapala modati kesulalo okati 1903loe paamu visham phosphatidilcolinnu lysophosphatidilcoling maarchinatlu kanugonabadindi, idhi kovvu aamlalalo okati laeni phosphatidilcolinga nirvachinchabadindi. 1960laloe, enjaimlu eee kovvu aamlam cheelikanu aneka vidhaluga utprerakam chestayani kanugonabadindi, vaatilo okati "sn"-1 sthaanam. eee pratyeka praticharya plaa1 dwara utprerakam cheyabadutundi, ayithe "sn"-2 sthaanam vadda praticharya phospholipase eah2 dwara utprerakam cheyabadutundi.

