tbe buffer

tbe ledhaa triss/borate/edaa, anedi triss bays, boric aamlam mariyu edaa misramaanni kaligi unna buffer draavanam.
molecular biologylo, tbe mariyu tay bufferlu tarachugaa nucleic aamlaalatoe kuudina vidhanalalo upayoginchabadataayi, veetilo atyanta saadhaaranamainadi electrophoresis. trice-assid draavanaalu konchem praathamika paristhitulaku samarthavanthamaina bufferlu, ivi dnnu deprotonate chesi, neetilo karigela chestaayi. edaa anedi divalent catayans yokka chelator, mukhyamgaa magnitium (mg2 +). kalushita nucleuslatho sahaa aneka enjaimlaku eee ayanlu avasaramaina saha-karakalu kabatti, edaa yokka paatra enzymatic ksheenata nundi nucleic aamlaalanu rakshinchadam. conny parimiti enjaimlu mariyu dna polymerase vanti aneka upayogakaramaina dna-savarinche enjaimlaku kooda kooda + saha-karakam kabatti, tbe ledhaa tay bufferlalo daani gaadata saadhaaranamgaa takkuvagaa unchabadutundi (saadhaaranamgaa sumaru 1 mimi vadda).
electrophoresis kosam iteevala kanugonna tbe mariyu tay bufferlaku pratyaamnaayaalu andubaatulo unnaayi.
vantakam (5x stock parishkaram yokka 1 leater).
hcl dwara pihech nii 8.3 kii sardubaatu cheyandi.
electrophoresislo upayoginche mundu tbe nii 1x kuu paluchana cheyavachu, 0.05x kooda aamodayogyamainadi. adhika saandratalu adhika ushna utpatti kaaranamgaa paelavamaina phalitaalaku daariteestaayi.

