
free-flow electrophoresis

vaahaka-rahita electrophoresis anie kooda piluvabade free-flow electrophoresis (ffe) anedi oka maatrika-rahita electrophoretic vibhajana saanketikata. ffe anedi kesanaalika electrophoresisku saaruupya saanketikata, polchadagina spashtatato, shaastreeya prasnalaku upayoginchavacchu, ikkada pakshika-sannahaka mariyu sannahaka parimaanamlo namuunaalu avasaram. chaarj ledhaa isoelectric paayintlooni vyatyaasaala prakaram namuunaalanu parimanatmakamga vaeru cheyadaaniki idhi upayoginchabadutundi. eee saanketikata yokka bahumukha pragna kaaranamgaa, arudaina loha ayanlu, protein isopharmlu, bahula protein complexelu, peptides, organels, kanaalu, dna origami, rakta seeram mariyu nanoparticals vanti namuunaalanu vaeru cheyadaaniki vistruta shreni protocols unnaayi. zell electrophorasis loe polyacrylamide vanti matricks avasaram lekunda drava draavanamloo karigina namuunaalanu vaegamgaa mariyu sunnitamgaa vaeru cheyadam anedi ffe yokka prayojanam. vislaeshanhalu yea vaahaka ledhaa maatruka nirmaanaaniki kattubadi undavu kabatti idhi chaalaa ekkuva recovery retunu nirdhaaristundi. daani nirantara swabhaavam mariyu adhika valume nirgamaamsa kaaranamgaa, eee saanketikata chaalaa ekkuva spashtatato sannahaka mottaala namuunaalanu vaegamgaa vaeru cheyadaaniki anumatistundi. antekakunda, vibhajanalanu sthaanika ledhaa vikruteekarinche paristhitulalo nirvahinchavacchu.
charitra.
ffeni 1960laloe germanyloni maaks-plank-institute vadda curt haneeg abhivruddhi chesaaru. 1980la varaku, idhi kanaalu mariyu kanajaalaala vibhajanaku praamaanika saanketikata, mariyu sunna gurutvakarshana kinda avakshepananu tagginchadaaniki antarikshamlo kooda ffe pareekshinchabadindi. flow cytometry kanaala kramabaddheekaranaku praamaanika paddhatigaa maaradamtho, ffe abhivruddhi proteins mariyu charge cheyabadina kanaala vibhajanapai drushti pettindi. konni samuuhaalu ffe vyavasthalu ledhaa micro eff la yokka chinna samskaranalapai kooda pani chestunnayi.
saanketikata.
vibhajana gadilo oka venuka palaka mariyu mundu palaka untaayi. byaak plate saadhaaranamgaa challabadina aluminium blacknu kaligi untundi, idhi plaastik kappabadina gaaju addamtho kappabadi untundi. mundu palaka eee rojullo pii. emm. emm. yea. thoo tayaaru cheyabadindi, munupati kaalamlo gaajunu upayoginchaaru. mundu mariyu venuka palaka madhya dooraanni spacerla dwara sardubaatu cheyavachu mariyu saadhaaranamgaa 0.01-0.5 mimi madhya untundi. mundu palakalo vibhajana bufferlu mariyu namuunaa kosam inletlu, fraction tubela kosam avutletlu mariyu electrodeluga platinum vairlu kooda untaayi. bahula buffer inletlapai ververu bufferlanu vartimpajeyadam dwara operator Ph, uppu saandratalu ledhaa kuurpunu marchagaladu, anduvalla gadhi vedalpupai vibhajana paristhitulu. 
vaeru chese bufferlu mariyu namuunaanu peristaltic pampula dwara, laminar pravahanni nirdhaarinchadaaniki vartimpajestaaru. adhika voltage vidyut kshetram laminar pravaahaaniki lambamgaa vartinchabadutundi. laminar pravaahamlooni vislaeshanhalanu charge saandrata mariyu/ledhaa isoelectric point dwara vaeru cheyavachu. daani atyanta bahumukha swabhaavam kaaranamgaa, eee saanketikata isotachophoresis, isoelectric focusing ledhaa (viramam) john electrophoresis vanti vividha electrophoresis paddhatulanu upayoginchagaladu. 
vibhajana kanam chivarilo, vaeru cheyabadina namuunaa binna gottala vadda vibhajinchabadutundi mariyu microtetor platelalo sekarinchabadutundi. aaa taruvaata namuunaalanu hetch. pii. ell. sii, ell. sii-emm. ess, maas spectrometry (eesi/maldi, upayoginchina protokaalnu batti) ledhaa electrophoresis (if/ess. dii. ess. paejee, 2dii-paejee) vanti anni pradhana paddhatula dwara vargeekarinchavachhu.
applications.
praamaanika anuvartanamlo protein complexelu, membrane proteins, protein mariyu antibody isopharmlu, kanaalu, upa kana compartmentlu (organels, ribozomelu vantivi) mariyu liposomla adhika-resolution vibhajana untaayi. ampholites dwara utpatti cheyabadina chaalaa chadunaina pihech-gradientlanu upayoginchadam dwara, 0.02 pihech unitla kante takkuvagaa unde protein isopharmlanu vaeru cheyadam saadhyamavutundi, veeti nirgamaamsamu gantaku 3 mii. graa. untundi.
resolutionnu penchadaaniki ledhaa namuunaalanu vikruteekarinchadaaniki bufferlaku sankalanaalanu jodinchadam kooda saadhyame. saadhaaranamgaa 8 mea varaku euria saandratalu, chaps, chapso, digitonin, dodessil-bs-dii-maltoside, octyle-bs-dii-glucoside, triton-ex-114 (if) mariyu dtt 50 mimi varaku vanti detergentlalo% thattukogalavu.
mukhya lakshanaalu.
 

