
sthaanika shosharasa kanupula vislaeshanha

murain lokal lymph node asse (llna) anedi charma sunnitatvam kosam "inn vivo" pareeksha.
ell. ell. enn. yea. ekkuvagaa ginia pig garishteekarana pareeksha mariyu beauhler pareekshanu adhigaminchindi. idhi marinta saastreeyamainadigaa mariyu takkuva krooramainadigaa pariganinchabadutundi (takkuva sankhyalo jantuvulu; takkuva baadha) mariyu vistruta shaastreeya mariyu niyantrana aamodaanni kanugondi.
llna antarleena suutram emitante, charma sensitizerlu shosharasa kanupulalo shosharasa kanupula perugudalanu prerepistaayi, idhi application saitnu paarudala chestundi. lymphocyte vistarananu radiolabelling (triteed thymidine parimaanam), bioluminisense (lymphocyteslo eah. tii. pii kantentnu parimaanam cheyadam) ledhaa immunoasse (elissa brdu kosam nirdishta antibodyni upayoginchadam) dwara kolavavacchu.
pareeksha samagri elukala chevulaku vartinchabadutundi. aichhikamgaa, 3h-methyl-thymidine ledhaa bradu vanti tracer padaardham lymphocyte vileenam kosam intraperitonialga inject cheyabadutundi. jantuvulanu anayasa chestaaru mariyu vaati shosharasa kanajaalam tolaginchi vislaeshistaaru. motaadu jantuvula nundi shosharasa kanupulalo tracer vileenam nishpattini niyantrana jantuvulato poolchutaaru, idhi uddeepana suuchika (sii) nuu istundi. uddeepana suuchika 3 (sii> 3) nuu daatinappudu, sambandhita sensitising sambhaavyatanu oohistaaru. shaastreeya ginia pandhi pareekshalaku viruddhamgaa, llna pareekshinchina rasayananni sunnitamgaa chese shakti yokka parimaanaatmaka kolatanu andistundi.
konni loha sammelanaalu, surfuctantlu, adhika-paramaanu-baruvu gala proteins, balamaina charma chikaku kaliginchevi mariyu chikitsa samayamlo aamodayogyamaina kaalaaniki cheviki taginantagaa kattubadi laeni padaarthaalaku llna taginadi kakapovachhu. eluka, ginia pandhi mariyu maanavulalo oka padaartham yokka sensitising potential loe sampoorna anugunyata ledhu.
niyantrana.
rasaayanaala pareeksha kosam maargadarsakaalu nem. 2010 juulai 23 naati 429.
reach regulation, anubandham VIII, paeraa 8.3 "murine lokal lymph node asse (llna) anedi" inn vivo "pareekshaku modati-empika paddhati. asaadhaarana paristhitulalo matrame maroka pareekshanu upayoginchaali. maroka pareekshanu upayoginchadaaniki samarthana andinchabadutundi."
european maargadarsakam okd 406 charma sunnitatvam prakaram, llna ledhaa mest (eluka chevi vaapu pareeksha) nuu charma sunnitatva sambhaavyatanu anchanaa veyadamlo modati dasagaa upayoginchavacchu. yea pareekshalonainaa saanukuula phalitam kanipiste, oka pareeksha padaarthaanni sambhaavya sensitizerga paerkonavacchu, inca tadupari ginia pig pareekshanu nirvahinchaalsina avasaram undakapovachhu. ayithe, llna ledhaa mestlo pratikuula phalitam kanipiste, ginia pig pareeksha (pradhaanyamgaa gpmt ledhaa biti) nirvahinchaali.