
pharmyelmethanophuran dehydrogenase

enjaimologylo, pharmyelmethanophuran dehydrogenase () anedi rasayana praticharyanu utprerakam chese enjaim.
eee enjaim yokka 3 uparitalaalu pharmyelmethanophuran, hetch2oo mariyu acceptor, ayithe daani 3 utpattulu carbon dye oxide, mithanophuran mariyu tagginchina acceptor.
eee enjaim oxidoriductasela kutumbaaniki chendinadi, mukhyamgaa daata yokka aldehyde ledhaa oxo samuuhampai itara sweekartalato panichesevi. eee enjaim taragati yokka kramabaddhamaina paeru pharmyelmethanophuran acceptor oxidoreductase. eee enjaimnu pharmyelmethanophuran (sweekarta) oxidoreductase anie kooda pilustaaru. eee enjaim folate biosynthesislo paalgontundi. dheeniki 2 cofactors unnaih malibdinum mariyu terrin.
aavishkarana mariyu jeevasambandhamaina sanghatana.
pharmyelmethanophuran (formail-mfr) dehydrogenase methanogenic archaealo kanipistundi, ivi carbon dioxide, formate, mithanol, methylamines mariyu acetate vanti uparitalaalanu upayoginchi meethennu samslaeshanha cheyagalavu. 
1967loe, methanogenla kosam hydrogen mariyu carbon dioxide yokka saamuuhika samskruti kosam nammadagina saanketikata abhivruddhi cheyabadindi. kilograamula kana sthaayini abhivruddhi chesi jeevarasaayana adhyayanala kosam upayoginchinanduna methanogenla jeevarasaayana saastramlo saha enjaimlu paalgontaayani spashtamaindi. "methanobacterium thermoatotrophicum" yokka hydrojantho carbon dioxide (CO2) taggimpu anedi atyanta adhyayanam cheyabadina vyavastha."" 
"methanobacterium thermoatotrophicum" yokka gvakriyalo methanogenesisloni daadaapu anni praticharyalu untaayi."formail-mfr kaligina malibdinum mariyu tungston m nundi vaeru cheyabaddaayi. thermoatotrophicum "avi karige cofactors-ksheeninchina kana saaraala nundi proteinlanu suddhi chesinappudu."" idhi prayogaaniki mundu unikilo undani teliyadu."carbon dye oxide karagani cofactors-ksheeninchina kana saaraala nundi meethennu utpatti cheyadaaniki mfr avasaram."formill-mfr dehydrogenase kooda" "mithanosaurcina barkeri" "mariyu archaeoglobus fulgidus kana saaraala nundi vaeru cheyabadindi"."malibdinum kaligina formail-mfr dehydrogenase" "methanothermobacter volfay" "nundi vaeru cheyabadindi".
nirmaanam.
2016loe, pharmyelmethanophuran dehydrogenase yokka ex-rae nirmaanam nirnayinchabadindi. formill-mfr rendu heterohexamerlanu kaligi untundi, ivi protein upabhaagaalu, ivi sii2 bhramana samaruupataloo cemetric diamerga anubandhinchabadataayi. formill-mfr dehydrogenase kooda varusaga diamer mariyu tetramer roopaallo 23 mariyu 46 iron-sulfur cubane samuuhaalanu kaligi untundi. sub unit efdabludlo dihydrorotescu saaruupyamaina rendu jink anuvulu untaayi. indulo enn6-carboxylicin, jink ligandlu mariyu utpreranaku keelakamaina aspartate kooda untaayi. intalo, sub unit eff. doubleu. dii. eff. naalugu tii-aakarapu ferrodoxin domainlatho koodi untundi, avi okela untaayi. eff. doubleu. dii. eff. upabhaagamlooni tii-aakarapu inumu-sulfur samuuhaalu bayati anchu nundi lopali core varaku oka marganni yerparustaayi. efdabludibidilo redox-active tungston untundi. 
efdabludibidiloni tungston naalugu diethioline theoletlatho samanvayam cheyabadutundi. sisis118 yokka theolet nundi aaru sulfurlu mariyu oka sendriya sulfide ligand efdabludibi yokka kriyaaseela pradaesamloe tungstapterin yokka tungstonku samanvayam chestaayi. tungston vakreekruta ashtatala jyaamitiloo samanvayam cheyabadutundi. carbon dioxide (CO2) kuu tagina bandhinche pradaesaanni christal yokka ex-rae nirmaanamloo draavakam aakramistundi, idhi vivolo undadu. binding sait cys118, hiss119, aargu228 mariyu sulfur-tungston ligand madhya undhi.
methanogenesis utprerakam.
formail-mfr dehydrogenase carbon dioxide (sioo2) nuu tagginchi carboxy-mfrnu yerparuchukovadam dwara methanogenesis praticharyanu utprerakam chestundi. ex-rae nirmaanam nundi pondina nirmaanaatmaka samacharam prakaram carbon dioxide (CO2) anedi 12.4 kii dhooramlo unna 4FE-4s ferredoxin (e = ~-500 mv) dwara fwdbd yokka tungstapterin kriyaaseela sait vadda carboxy-mfr kuu (e0 '=-430 mv) erpadataaniki tagginchabadutundi. appudu, idhi daani tungston ledhaa malibdinum kriyaaseela pradaesamloe carboxy-mfrnu mfrga taggistundi.
pratipaadita yantraangam.
43 samvatsaraala podavaina hydrophilic sorangam carbon dye oxide taggimpu mariyu sthireekarana yokka pratipaadita rendu-dasala drushtaamtaaniki maddatu istundi. eee hyrophilic sorangam efdabludibidi mariyu fdabluda kriyaaseela pradaesaala madhyalo undhi mariyu formic aamlam mariyu formate ravaanaaku [pica = 3.75] soukaryavantamgaa untundi. eee sorangam addankigaa kanipistundi, indulo irukaina maargam mariyu prati kriyaaseela pradaesam mundu bhaagamlo unna vistruta draavakam nindina kuharam untundi. addankula vadda eff. doubleu. dii. bii. dii. yokka aarr. zii. 228 mariyu eff. doubleu. dii. eah. niyantrana dwaaram operation yokka ell. ii. ess. 64. rendu [4Fe-4s] cluster golusu yokka bayati samuuham fwdf upabhaagaala saakhala bayati bhujamlo electronlanu tungston kendraaniki pamputundi. appudu, carbon dioxide fwdbd yokka hydrophobic sorangam dwara utpreraka compartmentloki pravesinchinappudu carbon dioxide formatega tagginchabadutundi (tungston aakseekarana cheyabadinappuduh tungston aakseekarana sthithi + 4 nundi + 6 kii velutundi). formic aamlam ledhaa formate hydrophilic sorangam dwara fdabluda yokka kriyaaseela pradaesaaniki vyaapistundi. okasari idhi kriyaaseela pradaesamloe vyaapinchina tarvaata, idhi anhu jink kendram vadda mfr thoo ghaneebhavistundi. taggina ferredoxintho erpadataaniki carbon dye oxide yokka exergonic taggimpunu saadhinchadaaniki sorangamloki pumping formate pratipaadinchabadindi.

