sim-simr taxin-antitoxin vyavastha

sim-cmr taxin-antitoxin vyavasthalo oka chinna sahajeevana endonuclease taxin, sim mariyu naan-coding rna sahajeevana rna antitoxin, cmr untaayi, idhi sim anuvaadaanni nirodhistundi. sim-simar anedi oka rakam ii taxin-antitoxin vyavastha, idhi antitoxin, simr dwara niyantrinchabadutundi. debbatinna rna mariyu dna recyclinglo sim-cmr complexes mukhyamaina paatra pooshistundani nammutaaru. sim mariyu simmer yokka sambandham mariyu sambandhita nirmaanaalu vishapuurita vidhanam mariyu procaryotic vyavasthalalo mottam paatra gurinchi antardrushtini andistaayi.
aavishkarana.
cmr modata ryjc anie paeru pettabadindi mariyu idhi σ70 pramotartho kuudina 77 nucleotide (nt) rna. raijesi vyatiraeka strandpy "visive" open reeding framenu 6 enti dwara ativyaapti chesinatlu kanugonabadindi, mariyu idhi antisense rnaga vargeekarinchabadindi, idhi "visive" yokka 5 'anuvaadam cheyani praantaanni bandhistundi. tadupari adhyayanam "yjiv" mariyu ryjc rendinti paerunu varusaga sim (chittadavi saaruupyatatoe sos-prerita yjiv janyuvu) mariyu simr gaa maarchadaaniki daariteesindi. aberb suupar familytho saaruupyatalu unnappatikee, sime kutumbam pratyaekamgaa proteobacterialo kanugonabadindi. 
sim mariyu simr madhya sambandham.
simmer antisense rna anedi sink prarambha codan venuka 3 entega lipyantareekarinchabadindi, anduke sink pravartakudu sink codan lopala ponduparachabadinatlu pariganinchabadutundi. phalitamgaa, simmer antisense binding dwara simm yokka rna anuvaadaanni nirodhistundi, idhi chivariki simmer marnah ksheenataku daariteestundani suuchistumdi. amaino assid vislaeshanha prakaram, transcription karakalu ledhaa antitoxins kaaranamgaa taxin lanty pravartananu pradarsinche rna cheelika proteinga sim parinaamam chendhi undavacchani nirdhaarinchindi. itara saadhaarana taxin-antitoxin vyavasthalaku bhinnamgaa, simmer antitoxin sim taxin kante marinta sthiramgaa untundi. 
dna debbatinna taruvaata, sos pratispandana simmer rna yokka lipyantareekarananu anichivestundi, dna marammatu cheyabade varaku sime taxin debbatinna mrnanu ksheeninchadaaniki anumatistundi. dheeniki viruddhamgaa, lexa repressor binding saitlu, simr mariyu loane protease dwara sim gattiga anachiveyabadutundi. eee moodu amsaalu bahula sthaayilalo unnaayi, ikkada lexa transcription down regulationlo paalgontundi, simmer rna translation down regulationlo paalgontundi mariyu loane protease protein ksheenatalo paalgontundi. saimpai anachiveta yokka paridhi lexa, simr mariyu loane protease yokka sankalana saktipai aadharapadi untundi. mottammeeda, sim samslaeshanha nemmadigaa untundi, endukante daani kaaryaacharana dna marammatu proteinlapy ekkuvagaa aadharapadi untundi. cellular vaataavaranamloo, mitomycin sii dnnu debbateestundi, idhi dna marammattunu praarambhinchadaaniki sim marnah yokka athi vyakteekaranaku daariteestundi.
vishapuuritam.
inn vitro" pareekshinchinappudu, sim yokka athi vyakteekarana kaalanee-erpade kanaala perugudalapai pratikuula prabhavalanu pradarsinchindi. kanamlooni prapancha anuvaadaanni anachiveyadam dwara, maroe vishamaina mazf maadirigaane marnanu cheelchadam dwara sim daani vishapuuritatanu pradarsistundi. parimaanaatmaka uttara maccha prayogaalu kanaalalo simar rna anedi sim mrna (0.02 efmol micro graa −1 mariyu 0.2 efmol micro graa −1) saandrata kante 10 retlu ekkuvagaa undani choopinchaayi. 
nirmaanam.
sime.
sim taxinlo 113 amaino aamlalu untaayi. amaino aamlam kramam mariyu sim yokka trutheeya nirmaanaanni anchanaa vesetappudu, aberb suupar familyni pooli unde balamaina saaruupyatalu kanugonabaddayi. eee suupar family pradhanamgaa transcription karakalu ledhaa antitoxinsga panichestundi; ayithe, aberb suupar family yokka praadhamika kramam mariyu trutheeya nirmaanaaniki sime yokka saaruupyata sime proteins transcription karakam ledhaa antitoxin nundi visha pravartananu pradarsinche rna-sambandhita proteinku parinaamaatmaka maarpunu anubhavinchaayani suuchistumdi. aberb suupar family protein nirmaanam mariyu sime protein nirmaanam madhya, protein madhyalo unna formula _ 1-helix mariyu formula _ 2 2 ekkuvagaa samrakshinchabadina aneka keelaka hydrophobic avasheshaalu unnaayi. eee keelaka saaruupyatalu unnappatikee, sime aberb suupar family yokka saadhaarana nirmaanamloo kanipinchani dhruva avasheshaalanu pradarsistundi, idhi eee avasheshaalu sime aarr. enn. yea cheelika saamardhyamlo paatranu kaligi undavacchani suuchistumdi.
swiss-namoonaalo aneka prayogaatmaka nirmaanaalu mariyu saiddhaantika homology namuunaalu unnaayi, ivi sim praadhamika kramam mariyu trutheeya nirmaanam yokka konni amsaalanu nirvachistaayi. uniprotcub accession number p39394 anedi sim taxin yokka saadhaarana nirmaanaanni suuchistumdi. swiss-modal syntheritical namoonaalo, formula _ 1-helix amaino aamlalu g44, q45, w46, l47, e48, a49, mariyu a50 kaligi untundi. formula _ 2 2 amaino aamlalu g55, t56, a57, v58, d59, v60, k61, v62, i67, v68, l69, t70, a71, q72, p73, mariyu p74 untaayi, ivi m63, e64, g65, mariyu c66 kaligi unna formula _ 2-turn kaligi untaayi.
sim.
simmer anedi antisense rna antey daani dviteeya nirmaanam vilakshanhamaina kaandam-mariyu-loop muulakaalatoe paatu nirmaanaaniki pakkane jatacheyani praantaalanu kaligi untundi. simar yokka anchanaa vesina dviteeya nirmaanam nucleotide sequence ccag kaligi unna loopnu pradarsistundi. eee lakshana loop lstr-1 mariyu ohechsi rna proteinlatho panchukoobadutundi mariyu itara proteinlaku binding saitga anchanaa veyabadindi. prastutam, arcsb protein dayta byaank ledhaa swiss-modal repositorylo cmr yokka anchanaa vesina trutheeya nirmaanaanni soochinche faillu aevee laevu. 

