
biomphalaria glabratalo efmrfomide

hridaya karyakalapala niyantranalo palgone neuropeptide ayina efmrfomide, maanava-sokina trematode paraannajeevi "skistosoma mansoniki" madhyantara atitheyamgaa paatraku prasiddhi chendina manchineeti natta jaathi ayina "biomphalaria glabrata" loe kanipistundi.
eee manchineeti natta jaatulanu oka namuunaa jeevigaa upayogistaaru, maroe maatalo cheppalante, oka maanavetara jaathi, idhi oka jeeva drugvishayaanni ardham chesukovadaniki vistrutamgaa adhyayanam cheyabadutundi, namoonaalo chesina aavishkaranalu itara jeevula paniteerupai antardrushtini andistaayane aasatoe. namuunaa jeevulu vivo namoonaalalo untaayi mariyu maanava prayogaalu asaadhyamaina ledhaa anaitikamainappudu maanava vyaadhini parisoedhinchadaaniki vistrutamgaa upayoginchabadataayi.
auchityam.
eee natta maanava pathology mariyu schistosomiasis yokka epidemiologyki sambandhinchi adhyayanam cheyabadindi. "ess. masoni "daani atidheyudini (" bii ") maarchadaaniki prasiddhi chendindi. shistosomine mariyu npi vanti neuropeptidela niyantrana/taggimpu dwara glabrata yokka) pravartana, mariyu konni adhyayanalu efmrfomide aminergic anie nivedinchaayi, mariyu paraannajeevulato sankramanaku pratispandinchadaaniki anuvula sraavamlo chikkukovacchu.
bii" yokka ganglionic central naraala vyavastha (cns). glabrata "loe jatha cheyabadina mastishka, pedal, pleural, parietal mariyu bukal ganglia mariyu oka jatha cheyani viseral ganglian untaayi. efmrfomide mastishka mariyu viseral ganglialo kendrikrutamai untundi, ayithe pindam abhivruddhi konasagutunnappudu efmrfomide natta thala-paada praantam nundi krindiki kadulutundani prastuta parisoedhana nundi labhinchina aadharalu suuchistunnaayi.
pinda kendra naadii vyavastha prarambha abhivruddhi samayamlo efmrfomide yokka khachitamaina paatra baga adhyayanam cheyabadaledu. eee neuropeptide gurtinchadam chaalaa mukhyam endukante daani vyakteekarana akasherukaalalo abhivruddhi prarambha dasallo cns punaadini vestundi. iteevali samvatsaraalalo, akasherukaalalo efmrfomide yokka neuromodulatory charyalu marinta spashtamgaa kanipinchaayi. cheruvu nattala yokka planorbidae mariyu limenidae kutumbaalapai chesina vistrutamaina adhyayanala kaaranamgaa idhi kontavaraku undhi.
b" loe fmrfamide vyakteekarana. glabrata "nuu 72 gantala praarambhamlo, cheelika taruvaata gurtinchavacchu. modati efmrfomide immunoriactive sell abhivruddhi chendina 25-28% vadda kanipistundi mariyu pindam yokka atyanta venuka bhaagamlo untundi kabatti, adhyayanalu ganglionic naadii vyavastha yokka venuka bhaagamlo daani vyakteekarana namuunaanu choopinchaayi. kanam prati vaipu oke prakriyanu pamputundi mariyu prati prakriya shareera vakratanu anusaristundi.
eee prakriyalu podigutunna koddi, shareera godaku iruvaipula rendu parshwa efmrfomide-vyakteekarana kanaalu spashtamgaa kanipistaayi. konni adhyayanalu efmrfomide eee nirmaanaalu trocopore larvalalo udbhavinchi abhivruddhi samayamlo vistarinche kandaraalanu prerepinchavacchani suuchimchaayi. eee neuromodulator hridaya karyakalapalanu niyantrinchadaaniki sahaayapadutundi. aneka efmrfomid sambandhita peptidelu telisinavi, ivi vividha cellular vidhulanu niyantristaayi mariyu anty-opiyet prabhavalu vanti aushadha charyalanu kaligi untaayi. efmrfomide osmore regulation mariyu development patterninglo kooda paatra pooshinchavachchu. eee neuropeoptide bahula vidhulanu kaligi untundi mariyu pindam vayojana nattagaa paripakvam chendadaaniki anumatinche aneka prakriyalanu niyantristundi.
bii" loe efmrfomide yokka immunocytochemistry. glabrata "pindaalu.
pindam jeevita kaalamlo efmrfomide namuunaa immunofluorescence dwara namodu cheyabadindi. cheelika tarvaata 0 nundi 144 gantala (6 rojulu) samayamlo pindaalanu guddu dravyarashi nundi teesi, gadhi ushnograta vadda 2 gantala paatu 4 saatam pfalo unchaaru. 4 rojula kante paatha pindaalanu praathamika antibody vyaaptini meruguparachadaaniki 5 nimishala paatu pbs loe 0.5 saatam ethyleniaminetetrasetic assid (edda) draavanamthoo mundugaane chikitsa chesi, aapai gadhi ushnograta vadda 2 gantala paatu 4 saatam pfalo amarcharu. pindaalannii ratriputa 4 saatam triton x-100loe pbsloe kadigi, nirodhinche draavanamloo podigevaaru, 1 saatam saadhaarana goal seeram pbsloe, ratriputa 4°C vadda.
ratriputa addukunna taruvaata, pindaalaku 1:2000 (ngs/pbs loe) vadda praathamika antibody draavanamthoo chikitsa chesaaru. floresin isotheocyanate (fitsi) thoo tagg cheyabadina dviteeya pratirakshakam-samyugmita anty robbit igg (polyclonal) nuu ratriputa 4 masim vadda 1:20 vadda podigevaaru. pindaalanu appudu oka slide meeda amarcharu mariyu fluorescent microscopytho chuusaaru.