
draveebhavana vakrata vislaeshanha

draveebhavana vakrata vislaeshanha anedi vaedi chesetappudu double-stranded dna yokka viyojana lakshanaala anchanaa. ushnograta perige koddi, double strand vidipovadam praarambhistundi, idhi shoshana teevrata, hyper chromicity perugudalaku daariteestundi. dnalo 50 saatam vikruteekarinchabadina ushnogratanu draveebhavana ushnograta antaaru. draveebhavana ushnogratanu kolavadam anedi draveebhavana ushnogratanu adhyayanam cheyadam dwara jaatulanu anchanaa vaeyadaaniki manaku sahaayapadutundi. endukante prati jeevi oka nirdishta draveebhavana vakrarekhanu kaligi untundi.
sekarinchina samacharanni single-nucleotide polymorphisms (snp) unikini mariyu gurtimpunu anchanaa vaeyadaaniki upayoginchavacchu. endukante zii-sii bays jatha cheyadam valla vaati madhya 3 hydrogen bandhaalu untaayi, ayithe eah-tii bays jatalaku 2 matrame untaayi. dna anedi eah ledhaa tii nundi sii ledhaa zii varaku utparivartanalu kaligi undatam valla adhika draveebhavana ushnogratanu srushtistundi.
eee samacharam DNAతో anuvu yokka paraspara charya vidhaanaaniki kooda mukhyamaina aadhaaraalanu istundi. intercollators vanti anuvulu bays jatala madhya slat avtayi mariyu paie stocking dwara sankarshana chendutaayi. idhi dna nirmaanampai sthireekarana prabhavanni chooputundi, idhi daani draveebhavana ushnograta perugudalaku daariteestundi. adevidhamgaa, uppu saandratalanu penchadam anedi dna vennemukalooni phosphatel madhya pratikuula vikarshanhalanu vyaapti cheyadaaniki sahaayapadutundi. idhi dna yokka draveebhavana ushnograta perugudalaku kooda daariteestundi. dheeniki viruddhamgaa, dna yokka sthirathvampai pihech pratikuula prabhavanni chooputundi, idhi daani draveebhavana ushnograta taggadaaniki daariteeyavachchu.
amalu.
rendu DNA tantuvula madhya bays-bays hydrogen bandhaanni vichchinnam cheyadaaniki avasaramaina shakti vaati podavu, gc content mariyu vaati paripuurakatapai aadharapadi untundi. double-stranded dna sequencelanu kaligi unna reaction-misramaanni vaedi cheyadam dwara mariyu ushnogrataku vyatiraekamgaa viyojanaanni kolavadam dwara, eee lakshanaalanu oohinchavachhu.
modata, yuvi shoshana kolatalanu upayoginchi strand dissociation gamaninchabadindi, conny fluorescence kolatala aadhaaramgaa paddhatulu ippudu atyanta saadhaarana vidhanam.
rendu dna-strandla madhya ushnograta-aadhaarita vibajananu sibr green, evagreen ledhaa fluorophore-label dna probes vanti dna-intercolating fluorophore upayoginchi kolavavacchu. sibar green vishayamlo (idhi dna yokka rendu tantuvula chinna gaadilo parasparam kalipinappudu 1000 retlu ekkuva teevramgaa fluoreses chestundi), vaedi chesetappudu dna yokka vibhajana fluorescence loe pedda taggimpu dwara kolavabadutundi. pratyamnaayamgaa, lakshya shreniki probe yokka paripuurakatanu nirnayinchadaaniki juckstapositioned probes (okati fluorophore mariyu marokati, tagina samanakam) nuu upayoginchavacchu.
draveebhavana-vakrata yokka pratikuula modati utpannam yokka graph, aaa vidhamgaa erpadina sikharaala kaaranamgaa, viyojana ushnogratanu (50 saatam viyojanamgaa nirvachinchabadindi) gurtinchadaanni sulabhataram chestundi.
1997loe lightsiclerlo cbr green enable product deferentiation. single-stranded (ss) probe-tuu-amplican hybrid nundi chaalaa nirdishta draveebhavana vakrarekhalanu andinchadaaniki hybridisation probes (ledhaa fret probes) kooda pradarsinchabaddaayi. light cycler parikaramlo eee viniyogaanni prachuryam pondadaaniki idaho saanketikata mariyu roche chaalaa chesaayi.
applications.
1990la chivari nundi sibr green, itara double-strand nirdishta rangula dwara utpatti vislaeshanha ledhaa probe-aadhaarita draveebhavana vakrata vislaeshanha daadaapu sarvavyaapti chendindi. probe-aadhaarita saanketikata single-nucleotide polymorphisms (snp) nuu gurtinchadaaniki taginanta sunnitamainadi mariyu utpatti cheyabadina dissociation namuunaala kaaranamgaa homozygus wild type, heterozygus mariyu homozygus utparivartana yugmavikalpala madhya thedaanu gurtinchagaladu. probes lekunda, amplican melting (mottam pcr utpatti yokka melting mariyu vislaeshanha) saadhaaranamgaa melting profiles dwara oke bays vaeriyantlanu kanugonadamlo vijayavantam kaaledu. adhika spashtata gala parikaraalu mariyu adhunaatana rangulatho, vaanijyaparamgaa labhinche aneka parikaraalatoe oka bays variantla amplican draveebhavana vislaeshanha ippudu saadhyamaindi. udaaharanakuh anuvartita jeeva vyavasthalu 7500 vegavantamaina vyavastha mariyu 7900h vegavantamaina real time pcr vyavastha, idaho technology yokka light scanner (modati plate aadhaarita hye resolution melting parikaram), kiazen yokka rotter-zeane parikaraalu mariyu roche yokka lightsicler 480 parikaraalu.
aneka parisoedhanalu mariyu clinical udaaharanalu saahityamlo unnaayi, ivi draveebhavana vakrata vislaeshanhanu kramabaddheekarana prayatnaalanu nivaarinchadaaniki ledhaa puurti cheyadaaniki upayoginchadaanni chuuputaayi, tadwara kharchulanu taggistaayi.
chaalaa parimaanaatmaka pcr yantraalaku draveebhavana vakrata utpatti mariyu vislaeshanha empika unnappatikee, vislaeshanha mariyu software maddatu sthaayi maarutuu untundi. adhika spashtata gala draavanam (hye-res draavanam ledhaa hcrm anie pilustaaru) eee saadhaarana saanketika parignaanam yokka purogati mariyu mottam rangu-tadisina amplican lopala snp gurtinchadaaniki adhika sunnitatvaanni andinchadam praarambhinchindi. idhi takkuva kharchutho kuudukunnadi mariyu sambhaavyata laeni draveebhavana vakrata vyavasthalanu abhivruddhi cheyadaaniki roopakalpanalo sulabhamainadi. ayithe, pedda mottamlo namuunaalanu proses cheyalsina janyu namuunaa anuvartanaala kosam, mottam nirgamaamsa mariyu vivarana soulabhyam kante abhivruddhi vyayam takkuva mukhyamainadi kaavacchu, tadwara probe-aadhaarita janyu namuunaa paddhatulaku anukuulamgaa untundi.

