
rna silencing suppressor pii19

rna silencing suppressor pii19 (tombuswirus pii19 core protein mariyu 19 kda symptum intensity modulator anie kooda pilustaaru) anedi tombuswirus janyuvulooni rf4 janyuvu nundi vyakteekarinchabadina protein. eee vairaslu mokkala kanaalaku sokina positive-sense single-stranded rna vairaslu, veetilo rna silencing vistrutamaina mariyu balamaina antiviral rakshana vyavasthanu yerparustundi. pii19 protein prati-rakshana vyuuhamgaa panichestundi, pratyaekamgaa rna nissabda vyavasthalo chinna jokyam chesukune rna (sirna) gaa panichese 19 nundi 21-nucleotide double-stranded rnalanu bandhistundi. sirnanu vaeru cheyadam dwara, p19 rna nissabdaanni anachivestundi mariyu viral vistarananu prothsahistundi. pii19 protein oka mukhyamaina virus karakamga mariyu mokkalu mariyu vaati vyadhikaaraka kaarakaala madhya parinaamaatmaka aayudha pootiiloo oka bhaagamgaa pariganinchabadutundi.
nirmaanam.
pii19 protein daani parimaanam nundi daani paerunu pondindi, idhi sumaru 19 kilodaltonlu. idhi oka functional homodymer nuu yerparustundi. tamota bushi stunt virus mariyu cornation italian ringspot virus nundi p19 proteinla christal nirmaanaalu andubaatulo unnaayi; protein oka kotta protein madatanu kaligi untundi mariyu beata sheet dwara erpadina binding uparitalaanni upayoginchi mariyu alfa helix dwara prakkane unna rnnu bandhinchadaaniki gatamlo teliyani yantraamgaanni udaharistundi, idhi 21 nucleotidela yokka double-stranded rnltho naan-sequence-nirdishta paddhatiloo sankarshana chendutundi.
function.
pii19 protein double-stranded rnltho bandhistundi, ivi chinna jokyam chesukune rna (sirna) gaa panicheestaayi mariyu enjaim dlc4 (dicerku homologytho kuudina mokkala enjaimla kutumbamlo sabhyudu) yokka 21-nucleotide utpattiki pratyekata kaligi untundi. sirnato bandhinchadam dwara, p19 eee jaatulanu kramabaddheekaristundi mariyu kanamlooni antiviral rna nissabda yantraamgaaniki madhyavartitvam chese protein complexes ayina rna-prerita nissabda samudaayam (risc) thoo sankarshana chendakunda nirodhistundi.
pii19 protein atitheya kanaaniki antarjanyudaina microrna anuvulatho, alaage chivariki virus yokka sonta janyuvu nundi udbhavinchina sirnasto kooda bandhinchagaladu. mukhyamgaa, eee namuunaaku minahaayimpu emitante, orgonout-1 (mundu 1) yokka vyakteekarananu anachivese niyantrana kaani coding rna ayina microrna mirr-168 thoo sankarshana chendadamlo pii19 yokka asamarthatha. rna nissabdam kosam munupati 1 protein avasaram, anduvalla pii19 yokka mirna yokka saadhaarana sequestration nundi daani repressornu empikagaa vidichipettadam cellular munupati 1 sthaayilanu tagginche prabhavanni kaligi untundi mariyu idhi pii19 nissabdaanni nirodhinche adanapu yantraangam. eee rendu vidhanalu okadaanikokati swatantramgaa untaayi mariyu utparivartana dwara empika cheyabadina vidhamgaa raddu cheyavachu.
parinaamam.
p19 proteinnu encoding chese janyuvu anedi atigaa mudrinchina janyuvuku oka udaaharana, idhi vairaslalo saadhaaranamaina janyu amarika, dheenilo bahula janyuvulu janyuvulooni oke bhaagam dwara encode cheyabadataayi, veetini pratyaamnaaya pathana framelalo chadavabadutundi. p19nii encode chese open reeding frame orf4, maroka janyuvu yokka open reeding framelo puurtigaa untundi, idhi orf3gaa perkonabadindi mariyu kadalika protein p22nii encode chestundi. janyuvulu mariyu vaati saapeksha sthaanaalu rendoo, tombuswirus kutumbamlo samrakshinchabadataayi. p19 eee vamsamlo "dii novo" udbhavinchindani bhaavistunnaaru.
dsrna yokka sequestration anedi rna silencingku vyatiraekamgaa oka saadhaarana viral counter-defence vyuuham, idhi virus mariyu host madhya parinaamaatmaka aayudhaala pootii roopamlo abhivruddhi chendindi. eee paatralo p19 protein pratyekamainadi kaadu; convergent evalution yokka udaaharanalo, eee vyuuham vibhina nirmaanaalu mariyu rnanu bandhinche bhouthika maargaalato proteinlanu upayoginchi vibhina viral vamsaalaloo kaneesam moodu saarlu abhivruddhi chendinatlu kanipistundi.
charitra.
tamota bushi stunt virus, idhi tombuswirus kutumbaaniki chendina rakam, idhi mokkala vairasla adhyayanam kosam deerghakaala namuunaa vyavastha. open reeding frame encoding p19 modata 1980la chivaralo virus yokka janyuvunu kramabaddhikarinchinappudu kanugonabadindi; virus mariyu infectivityni prothsahinchadamlo daani paatra modatlo takkuvagaa prasamsimchabadinappatikii, anchanaa vesina protein nijamgaa janyuvu nundi vyakteekarinchabadindani taruvaata niroopinchabadindi. rna silencing yokka anichivetagaa daani paatranu spashtam chesina taruvaata, rna silencing, rna jokyam mariyu sambandhita prakriyalapai molecular biology parisoedhanaloo pii19 kooda oka saadhanamgaa upayoginchabadindi.

