
yuu7-actinitoxin-pn1eah

yuu7-stenitoxin-pn1eah (ledhaa sankshiptamgaa yuu7-cntx-pn1eah) anedi tripivi1 chaanellanu nirodhinche neurotoxin, mariyu idhi noppini kaliginche prabhavalanu pradarsinchagaladu. idhi sahajamgaa "phoneutria nigreventor" yokka vishamlo kanipistundi.
vyutpatti.
taxinku pratipaadita paeru yuu7-actinitoxin-pn1eah. ikkada, 'citanitoxin' anedi "citanidae" kutumbaaniki chendina salepurugula vishamlo kanipinche vishanni suuchistumdi. pn' anedi "phoneutria negreventor" kuu sankshipta roopam, idhi verucheyabadina jantuvu yokka jaathi mariyu jaatulanu suuchistumdi.
yuu7-cntx-pn1eki saadhaaranamgaa upayoginche pratyaamnaaya paeru pntx3-5, idhi "phoneutria nigreventor" taxin 3-5kii sankshipta roopam. ikkada, 3 anedi zell filteration mariyu riverse faze protein faast liquid chromatography kalayikanu kaligi unna saanketikatanu upayoginchi visham nundi verucheyabadina moodava vishapuurita protein bhinnamlo bhaagamgaa suuchistumdi. adevidhamgaa, 5 anedi eee bhinnam nundi riverse faze mariyu ayan-exchange hye-performance liquid chromatography dwara vaeru cheyabadina aidava peptide anie suuchistumdi.
muulaalu.
yuu7-cntx-pn1eah sahajamgaa "phoneutria nigreventor" yokka vishamlo kanipistundi, dheenini tarachugaa brazilian vandaring ledhaa 'saayudha' saaleedu anie pilustaaru. adanamgaa, isolated neurotoxin nundi pondina amaino assid sequence aadhaaramgaa recombinant protein utpatti cheyabadindi.
chemistry.
nirmaanam.
yuu7-cntx-pn1eah anedi oka 5063.6 zii/moll, 45 amaino assid protein (uniprote pii81791). paripakva protein yokka amaino aamlam kramam ilaa untundih
 0 gcigrnessck fdrhgCCwpw scancake pesdiviewsex lkigk 45
neurotoxin yokka oohinchina nirmaanam naalugu disulfide bandhaalanu kaligi untundi, ivi eee krindi paddhatiloo amarchabadi untayih 1-4,2-5,3-8,6-7. disulfide bandhaalalo moodu inhibitor systeine mudi yerparustaayi, idhi vaedi vikruteekarana mariyu proteolysis nirodhakatanu penchutundani nammutaaru. transcriptomics oka enn-terminal signal peptide mariyu proo peptide rendinti unikini velladinchindi, ivi anuvaadam tarvaata cheelchabadataayi.
kutumbam.
yuu7-cn tx-pn1eah anedi cstx kutumbamlo sabhyudiga pariganinchabadutundi. eee kutumbamlo pradhanamgaa "cupiennius saeli" vishamlo kanipinche vishalu unnappatikee, yuu7-cntx-pn1eah kooda daani atyanta saaruupya disulfide bandham nirmaanam kaaranamgaa cherchabadindi.  
lakshyam mariyu paramaanu yantraangam.
rtrpv1 thoo sankraminchina 293 kanaalapai u7-cntx-pn1a pareekshinchabadinappudu, capsicin grahakam, prerita antargata ca2 + pravahalu nirodhinchabaddaayi. u7-cntx-pn1a trigiminal ganglian nundi glutamate vidudalanu nirodhinchindi. idhi kalisi taxin anedi trpv1 grahakalanu nirodhistundani suuchistumdi. empika chesina trpv1 blocker ayina sb-366791 thoo poliste, vishamu ekkuva nirodhaka saktini chooputundani kanugonabadindi, endukante u7-cntx-pn1a yokka chaalaa takkuva ekagrata avasaram, 47 ± 0.18nm, 45 ± 1.18nm mariyu 390 ± 5.1nm yokka IC50 thoo sthaanika, recombinant u7-cntx-pn1a mariyu sb-366791, varusaga. adanamgaa, neuropathic noppi namoonaalo ell-rakam voltage-gated calcium chhaanals (vijisisi) nuu ell-rakam vijisisi blockers upayoginchi lakshyamgaa pettukoodaaniki taxin kanugonabadindi. elukalaku (30 efmol/sait, antey.tii.), ayithe, khachitamaina antarleena yantraangam inca kanugonabadaledu.
vishapuuritam.
intracerebroventracularic injection dwara 5 pg/mousnu aplai chesinappudu, u7-cntx-pn1a elukala posterior avayavaalalo pakshavaataaniki kaaranamavutundani kanugonabadindi. kadalikalu mariyu dookudu 24 gantallo taggadam gamaninchabadindi.
chikitsa upayogam.
yuu7-cntx-pn1eahnuu clinical settinglo analgesic mariyu anty-nociseptionga ledhaa trpv1 chaanal kutumbaanni adhyayanam cheyadaaniki aushadha saadhanamgaa upayoginchavacchu. vividha noppi namoonaalalo idhi vividha sthaayilalo antinocyptive prabhavalanu pradarsinchindi. operation anantara noppiloo kotha namoonaalo, idhi janthu namoonaalalo yaantrika hyperologesianu tagginchindi (30-300 fmol/sait, i.tii.). adanamgaa, U7-cntx-pn1a (30 fmol/sait, i.) rojuvaaree upayogam tarvaata sthiramaina antinosyseptive prabhavalanu kolustaaru.tii.), mariyu, neuropathic noppi yokka pakshika sciatic naraala bandhana namoonaalo, yaantrika hyperalgesia yokka swalpakaalika taggimpu gamaninchabadindi (1h). U7-cntx-pn1a yokka atyanta aasaajanakamaina mariyu vinootnamaina chikitsa upayogam cancer-sambandhita noppi-namoonaalalo undhi, idhi elukalalo vartinchabadutundi. 30 efmol/sait, antey.tii. marphin sahanam abhivruddhi chesina elukalaku kooda eee elukala namoonaalalo yaantrika hyperalgesia tagginchabadindi.

