
chromosome jumping

chromosome jumping anedi janyuvula bhouthika mappinglo upayoginche molecular biology yokka sadhanam. idhi chromosome nadakatho sahaa adhe prayojanam kosam upayoginche aneka itara saadhanaalaku sambandhinchinadi.
chromosome jumping anedi pratiroopam cheyataaniki kashtamaina praantaalanu daataveyadaaniki upayoginchabadutundi, punaraavruta DNA kaligi unnavi, chromosome nadaka dwara sulabhamgaa mapp cheyalemu mariyu oka nirdishta janyuvu kosam vetakadamlo chromosome venta vaegamgaa kadaladaaniki upayogapadutundi. chromosome nadaka vale kakunda, chromosome jumping anedi madhyavartitva kramaanni cloning cheyakunda adhe chromosome yokka sambhaavya suduura binduvunu daatadaaniki chromosome yokka oka binduvupai praarambhinchagaladu. pedda dna sakalam yokka chivaralanu chromosome jumping yokka lakshya cloning vibhagam, ayithe madhya vibhagam cloning dasaku mundu rasayana taarumaaru varusa dwara tolaginchabadutundi. 
prakriya.
chromosome jumping anedi madhya vibhagam lekunda dna kramam yokka rendu chivaralanu clone cheyadaaniki veelu kalpistundi. janyu DNA nii parimiti endonuclease upayoginchi paakshikamgaa jeernam cheyavachu mariyu DNA ligase sahayamtho, sakalaalu takkuva gaadatatoo vruttaakaaramlo untaayi. telisina kramam nundi, vruttaakaara junction antataa kramam cheyadaaniki primer roopondinchabadindi. eee primer 100 kb-300 kb viramalanu dookadaaniki upayoginchabadutundih 100 kb dhooramlo unna kramam vruttaakaaramlo telisina kramaniki daggaragaa vacchi untundi, idhi pratyaamnaaya paddhatiloo dookadam mariyu kramabaddhikarananu anumatistundi. anduvalla, chromosome nadaka dwara cherukoleni kramaanni kramabaddheekarinchavachhu. janyuvu lanty kramaanni vetakadaaniki kotta jump sthaanam nundi (yea disalo ayina) chromosome nadakanu kooda upayoginchavacchu, ledhaa chromosome venta marinta purogati saadhinchadaaniki adanapu jamplanu upayoginchavacchu. chromosome dwara chromosomecu jumping cheyadam dwara chromosome dwara nadiche lakshya janyuvu yokka shodhana kosam punaraavruta DNA nii daataveyadaaniki anumatistundi.
library.
cloning dasaku mundu amalu cheyabadina taarumaaru kaaranamgaa chromosome jumping library chromosome walking nundi bhinnamgaa untundi. chromosome jumping libraryni nirminchadaaniki, vyaktigata klonelu janyuvulooni yaadruchchika binduvula nundi udbhavistaayi (saadhaarana jumping librarylu modati praathamika protocol) ledhaa nirdishta parimiti sakalaala (nirdishta jumping librarylu pratyaamnaaya protocol) mugimpu nundi gurtinchaali. 
noti-digested dna.
libraryni nirminchadaaniki oka udaaharana notai vanti arudaina-cutting parimiti endonuclesga vargeekarinchabadindi. noti-digested human dna aadhaaramgaa libraryni nirminchadaaniki mariyu vargeekarinchadaaniki, yaadruchchika klonelu parimiti mapping dwara vislaeshimchabaddaayi. notito puurti jeernakriya dwara tayaaraina sakalaala parimaanaala vistruta pampinhee kaaranamgaa, libraryni takkuva mariyu adhika plasmid ekagrata anae rendu bhinnaluga nirminchaaru. pratyaekamaina tudi sakalaalanu kaligi unna clonelanu pulls feeled gradient (pfg) sadaran blats kuu hybridisation dwara vislaeshimchaaru. noti, bisshi mariyu nrui enjaimlatho maanava dna yokka single mariyu double digestla kosam sekarinchina phalitaalanu pariseelinchi, 850 kebi thoo kuudina parimiti mapp linking mariyu jumping clonelanu kaligi unna praantam srushtinchabadindi. inca, 250 mariyu 350 kebi jumps yokka noti sakalaalu 0.25 mariyu 0.35 sem. mea. janyu dooraalaku anugunamgaa utpannamaina rendu chivarala clonelalo spashtamgaa kanipinchaayi.
prayojanaalu mariyu aprayojanaalu.
chromosome jumping yokka prayojanaaluh
ayithe, eee prayojanaalu unnappatikee, chromosome jumping ippatikee cloning vector saamarthyam dwara parimitam cheyabadindi, idhi rendu sakalaala chivarala dhooram, idhi sumaru vandala kilobesla kaavacchu. adanamgaa, jump madhya unna dnnu clone cheyadu kabatti, dnalo unna anni janyuvulanu gurtinchadaaniki chromosome walking cheyavalasi untundi. sambandham lekunda, chromosome nadakatho poliste vanda kilobeslaku paiga dhooke avakaasam unnanduna idhi ippatikee prayojanakaramgaa pariganinchabadutundi.
applications.
janyu rugmatalu.
chromosome jumping librarylu pedda paramaanu dhooraalatho praamaanika cloning paddhatula sanklishtatanu parishkarinchadamlo sahaayapadataayi. eee prakriya 100 kilobesla jumps avasaramayye itara janyu rugmatalaku chromosome jumping libraryni upayoginche avakaasaanni anumatinchindi. mukhyamgaa systic fibrosis vanti janyu rugmatalaku, daani janyuvu maanava chromosome 7loe undhi, chromosome jumping libraryni upayoginchi jumping clone kosam vetakagaligindi, idhi ankogene nuu kalusukunnadi. coding (exanlu) mariyu naan-coding (intraanlu) vibhaagaalato koodi unna eucariotic janyuvulalo undatam valla systic fibrosis gurtinchadam sanklishtamgaa undhi, ikkada intraanlu parimaanamlo chinnaviga untaayi, tadwara vaatini gurtinchadam kashtam. systic fibrosis janyuvunu gurtinchadamlo maroka poraatam emitante, ksheerada kanaalu vividha rakaala punaraavruta dnlanu kaligi untaayi, ivi tappu cloning mariyu dna replication yokka addanki mariyu asthirataku daariteestaayi. eee rendu samasyalu, saampradaaya cloning paddhatulu proses cheyalevu endukante systic fibrosis janyuvunu gurtinchadaaniki oka sanketaanni utpatti cheyadaaniki exanla pedda digubadi kanipinchaalsi untundi mariyu DNA punaraavruta muulakaala nundi vimukti kaligi undaali. 

