collagen hybridising peptide

collagen hybridising peptide (chp) anedi saadhaaranamgaa gly-xaa-ya amaino aamlam triple yokka 6 nundi 10 punaraavruta unitlatho kuudina synthetic peptide kramam, idhi sahaja collagenla hallmark kramaanni anukaristundi. oka chp peptide saadhaaranamgaa xaa mariyu yaa sthaanaalalo proline mariyu hydroxyproline yokka adhika kantentnu kaligi untundi, idhi collagen yokka pratyaekamaina triple helix conformation roopondinchadaaniki balamaina pravruttini istundi. oke-stranded (monomeric) sthithilo, peptide collagen strandlatho hybridised triple helixnu roopondinchadam dwara kanajaalaalo vikruteekarinchina collagen strandlanu gurtinchagaladu. idhi triple helical chain assembly mariyu inter-chain hydrogen banding dwara sambhavistundi, idhi pcr samayamlo karigina DNA strandlaku primerlu bandhinche paddhatiloo jarugutundi. bandham kollajenpai nirdishta kramam ledhaa epitope meeda aadharapadi undadu, vividha rakaala collagen golusulanu lakshyamgaa chesukovadaniki chps nii anumatistundi.
collagen, chp, cmp, mariyu clp.
collagen anedi extracellular matricks (ii. sii. emm) yokka pradhana bhaagam. collagen suupar familylo 28 rakaala kollaajenlu untaayi. eee collagenla paniteeru mariyu kramaanugata nirmaanam maaravacchu, ayithe avanni triple helix anie piluvabade nirvachinche nirmaana lakshanaanni panchukuntaayi, ikkada moodu edama chethi polyproline II-rakam (ppi) helislu kudi chethi suupar coiled helical muulaamsaanni roopondinchadaaniki samavesamavutayi. collagen mimetic peptides (sii. emm. pii. ess) ledhaa collagen lanty peptides (sii. ell. pii. ess) anie piluvabade chinna synthetic peptides collagen triple helix yokka nirvachinchina nirmaanam, daani madata gatisastram mariyu chinna triple helical namoonalugaa ushna sthirathvaanni spashtam cheyadamlo pradhana paatra poeshinchaayi. sii. emm. pii. luu, clips mariyu chips annee vaati amaino assid sequencela paramgaa chaalaa pooli untaayi, conny sii. emm. pii. luu ledhaa clups vaati draveebhavana ushnogratala kante ekkuvagaa vaedi cheyabadinappudu matrame, avi vidipoyina, single-stranded sthithilo unnaya mariyu vaatini chipsga pariganinchavacchu.
bandhinche vidhanam.
single-stranded chips itara lakshya yantraamgaala nundi pratyaekamaina paddhatiloo denatured collagen chains mariyu jelatinku bandhistundi, udaaharanaku, monoclonal antibodies (mabs) kosam kanipinche nirdishta epitopulanu bandhinchadaaniki viruddhamgaa, avi madata mariyu golusu assembly kosam pratyaekamaina nirmaanaatmaka muulaamsaanni (collagen triple helix) gurtistaayi. vaati pratyaekamaina lakshya yantraangam kaaranamgaa, chips vikruteekarinchina collagen golusula patla adhika bandham nirdishtatanu kaligi untundi, conny chekkuchedarakunda (triple helical) kollajenku daadaapu etuvanti anubandham undadu. ushna, rasayana, yaantrika ledhaa enzymatic prakriyala dwara vikruteekarinchabadina collagen golusulanu, alaage bahula collagen rakalanu (udhaa.zii., cole i, ii, iv). adhyayanalu kooda chips mariyu vaati fluorophore kanjugetlu seeram thoo sambandhamlo unnatamaina sthirathvaanni kaligi unnayani choopinchaayi.
kanajaala punarnirmaanam mariyu nashtam kosam biomarkerga denatured collagen.
niyantrita collagen turnover pindam abhivruddhi, avayava roopajananamtho paatu kanajaala nirvahana mariyu marammattuku keelakam. ayithe, collagen homeostasis yokka maarpulu aneka vyaadhulu mariyu rogalakshana paristhithulatho mudipadi untaayi. adhika collagen ksheenata cancer metastasis, charma vruddhaapyam, arthuritis mariyu bolu emukala vyaadhiki sambandhinchinadi kaavacchu. triple helix nirmaanam dwara ksheeninchina mariyu vippabadina collagen tantuvulatoe bandhinche saamarthyam aadhaaramgaa punarnirminchabadutunna kanajalalanu chps lakshyamgaa chesukovacchu. lakshyamaina bhaagamgaa, chips hystopathology, diagnostics mariyu vistruta shreni vyaadhulaku aushadha pampinheelo goppa saamarthyaanni andistundi.
vyaadhi sthithulalo collagen vikruteekarananu anchanaa vaeyadaaniki chaalaa paddhatulu parokshamgaa untaayi, udaaharanaku matricks metaloproteiness (emm. emm. pii) karyakalapalanu gurtinchadam ledhaa moothram, seeram ledhaa sinovial dravam loe collagen peptide sakalaalanu lekkinchadam. collagen nuu neerugaa lakshyamgaa chesukovadaniki sampradaaya paddhatulanu upayoginchi, parisoedhakulu faze display dwara empika cheyabadina collagen binding peptides, collagen binding proteinla nundi udbhavinchinavi ledhaa kollaajenlaku vyatiraekamgaa perigina pratirodhakaalapai aadharapadali. duradrushtavasaattu, eee sammelanaalu nirmaanaatmakamainavi kaani mariyu nirvachinchina nirvachinchina epitopnu pradarsinchani vikruteekarinchina kollaajenlanu lakshyamgaa chesukolevu. adanamgaa, nirdishta adhokaranam chendina collagen sakalaalanu vaeru cheyadaaniki nivedinchabadina pratiroedhakaalu okati ledhaa konni collagen rakalanu matrame gurtinchagalavu. dheeniki viruddhamgaa, chps, suutrapraayamgaa, anni rakaala vikruteekarinchina collagenlatho bandhinchagaladu.
applications.
kanajaalam marakalu.
fluorofore-ledhaa biotine-label cheyabadina chips nuu collagen ksheenata mariyu denaturation immunofluorescence mariyu immunohystochemistry applicationla dwara gurtinchadaaniki oka steining agentga upayogistaaru. chii. pii. ess. ghaneebhavinchina kanajaala vibhagalu, formalin-fixed paraphine embeded (eff. eff. pii. ii) vibhagalu, alaage taja kanajalalanu maraka cheyavachu. bahula jaatula nundi kanajaala namuunaalaku mariyu myocardial inforction, arthuritis, nephritis mariyu fibrosis vanti aneka rakaala vyaadhulaku chp vartistundi.
vivo imaging loe.
inn vivo" fluorescent imaging kosam nrpsnu nier-infrared fluoroforlatho kooda label cheyavachu.
collagen gurtimpu.
sds-paejee jellalo aneka rakaala collagen byaandlanu drushyamaanam cheyadaaniki chipsnu upayoginchavacchu. jelnu load cheyadaaniki mundu esdeela samakshamlo vaedi cheyadam dwara collagen vikruteekarinchabadutundi. jellu fluorescent-label cheyabadina chips dwara marakalu padinapudu collagen byaandlu chipi-collagen hybridisation dwara drushyamaanam cheyabadataayi.
anusandhaana kanajaalaaniki yaantrika nashtanni gurtinchadam.
collagen sariiramlooni snaayuvulu, snaayuvulu mariyu emuka vanti load baring kanajaalaalo yaantrika balaanni andistundi. eee kanajaalaalaku balaalu vartimpajeyabadinanduna, collagen triple helix debbatintundi mariyu visraantini pondavacchu, mariyu atuvanti bandhana kanajaalaalo yaantrika nashtanni paramaanu sthaayi gurtinchadaaniki chips anumatistundi.

