
pump-probe microscopy

pump-probe microscopy anedi rasayana praticharyalanu adhyayanam cheyadaaniki femtochemistrylo upayoginche naan-leanier optical imaging paddhati. idhi antarjanyuyetara fluorescent lakshyaala nundi adhika-viruddha chitraalanu utpatti chestundi. idhi materials sains, medicine mariyu art punaruddharanatho sahaa aneka anuvartanaalanu kaligi undhi.
prayojanaalu.
suukshmadarshini saastravettalu upayoginche naan leanier shoshana yokka classic paddhati sampradaaya rendu-photan fluorescence, dheenilo oke muulam nundi rendu phootaanlu photoelectronnu uttejaparichenduku sankarshana chendutaayi. electron daani ground state kii tirigi maarutunnappudu oka photan nuu vidudala chestundi. eee suukshmadarshini paddhati daani antarleena trimiteeya optical vibhajana saamarthyaala kaaranamgaa jeeva saastraalalo viplavaatmakamainadi.
rendu-photan shoshana anedi antargatamgaa oka naan leanier prakriyah fluorescent avtput teevrata anedi excitation kaanti teevrata yokka vargaaniki anulomaanupaatamlo untundi. idhi pratideepti lazer punjam yokka kendra binduvu lopala matrame utpatti avtundani nirdhaaristundi, endukante eee plain velupala teevrata photoelectronnu uttejaparachadaaniki saripodu.
ayithe, eee suukshmadarshini paddhati rendu-photan excitation mariyu fluorescence rendintikee lonayye jeeva anuvula sankhya dwara antargatamgaa parimitam cheyabadindi.
pump-probe microscopy neerugaa excitation kaantini kolavadam dwara eee parimitini adhigamistundi. idhi vishraanti teesukunna tarvaata fluoreses kakapoyina, rendu-photan shoshana saamarthyam unna edainaa anuvuku sambhaavya lakshyaala sankhyanu vistaristundi. lakshya anuvunu uttejita sthithiki teesukuraavadaaniki eee paddhati "pump" anie piluvabade pulsed lazer punjam yokka vaisaalyaanni niyantristundi. idhi anuvutho rendu kiranaala paraspara charya aadhaaramgaa "probe" anie piluvabade rendava pondikaina punjam yokka lakshanaalanu prabhaavitam chestundi. eee lakshanaalanu oka detector dwara kolichi oka chitraanni roopondistaaru.
pump-probe microscopy yokka bhouthika shaastram.
pump-probe microscopy fluorescent lakshyalapai aadhaarapadadu kabatti, eee paddhati bahula rakaala multifotan shoshananu sadviniyogam chesukuntundi.
rendu-photan shoshana.
rendu-photan shoshana (tpa) anedi moodava-kramam prakriya, dheenilo rendu phootaanlu oke anuvu dwara daadaapu ekakaalamlo grahinchabadataayi. adhe quantum sanghatanalo adhe electron rendava photaannu grahinchinatlayithe, electron uttejita sthithiloki pravesistundi.
idhi rendu-photan microscopy (tii. pii. emm) loe upayoginche adhe drugvishayam, conny pump-probe microscopyni tii. pii. emm nundi vaeru chese rendu mukhya lakshanaalu unnaayi. modata, anuvu fluorescent kanavasaram ledhu kabatti, photo detector probe teevratanu kolustundi. anduvalla, rendu-photan shoshana sambhavinchinappudu signal taggutundi, idhi TPM kii viruddhamgaa untundi.
rendavadi, pump-probe microscopy prati photanku varnapatamgaa vaeru cheyabadina muulaalanu upayogistundi, ayithe sampradaaya tii. pii. emm oke tarangadairghyam yokka oka muulaanni upayogistundi. dheenini degenerate too-photan excitation anie pilustaaru.
uttejita-sthithi shoshana.
pump punjam oka electronnu uttejita sthithiloki pampinappudu uttejita-sthithi shoshana (esa) sambhavistundi, appudu probe punjam electronnu adhika uttejita sthithiki pamputundi. idhi pradhanamgaa idhi sambhavinche kaalaparimitilo tpa nundi bhinnamgaa untundi. endukante oka electron nanoseconla vyavadhi varaku uttejita sthithilo undagaladu, anduvalla tpa kante ekkuva pulls vyavadhi avasaram.
uddeepana udgaaraalu.
pump-probe microscopy kooda uddeepana udgaaraalanu kolavagaladu. eee sandarbhamlo, pump punjam electronnu uttejita sthithiki nadipistundi. appudu electron probe punjanku gurainappudu photaannu vidudala chestundi. eee paraspara charya detector sait vadda probe signalnu penchutundi.
bhuu-sthithi ksheenata.
pump punjam electronnu uttejita sthithiloki pampinappudu ground-state ksheenata sambhavistundi. ayithe, esa maadirigaa kakunda, probe punjam oka electronnu dviteeya uttejita sthithiloki pampadu. badulugaa, idhi migilina electronlanu ground state nundi modati uttejita sthithiki pamputundi. ayithe, pump punjam naela sthithilo electronla sankhyanu tagginchinanduna, takkuva probe phootaanlu grahinchabadataayi mariyu detector sait vadda probe signal perugutundi.
cross-faze modulation.
cross-faze modulation anedi kerr prabhavam valla sambhavistundi, dheenilo pedda vidyut kshetram samakshamlo namuunaa yokka vakreebhavana suuchika maarutundi. eee sandarbhamlo, pump punjam probe yokka dasanu modulate chestundi, appudu dheenini interferometric paddhatula dwara kolavavacchu. konni sandarbhaallo, cross-faze modulation spectral shifting anie pilustaaru, eee faze maarpu spectral filtertho gurtinchagala pump spectrumlo maarpunu prerepistundi.
optical design.
utsaaham.
naan leanier optical paraspara charyalanu kolavadaniki adhika sthaayi takshana shakti mariyu chaalaa khachitamaina samayam avasaram. sunnitamaina namuunaala nashtanni nivaaristuu, eee paraspara charyalanu utpatti cheyadaaniki avasaramaina photanla adhika sankhyanu saadhinchadaaniki, eee suukshmadarshinulaku namuunaa lazer avasaram. eee laejarlu femtosecond samayaparimitilo chaalaa ekkuva photan gananalanu saadhinchagalavu mariyu takkuva sagatu saktini nirvahinchagalavu. chaalaa vyavasthalu tie saaph geyin meediyamnu upayogistaayi, endukante idhi access cheyagala vistruta shreni tarangadairghyala kaaranamgaa.
saadhaaranamgaa, pampu mariyu probnu utpatti cheyadaaniki adhe muulam upayoginchabadutundi. probe punjamnu kaavalasina tarangadairghyaaniki maarchadaaniki optical paraamiteeya ausilator (opo) upayoginchabadutundi. spectroscopic anuvartanaala kosam probe tarangadairghyaanni pedda shrenilo tune cheyavachu.
ayithe, konni rakaala rendu-photan paraspara charyalaku, pratyeka pulsed muulaalanu upayoginchadam saadhyamavutundi. uttejita-sthithi shoshana vanti paraspara charyalatho matrame idhi saadhyamavutundi, dheenilo electronlu aneka picosecondla paatu uttejita sthithilo untaayi. ayithe, pump mariyu probe kiranaala madhya samayanni savarinchadaaniki ververu podavula rendu ververu punjam maargaalato oke femtosecond muulaanni upayoginchadam sarvasaadhaaranam.
pump punjam vyaapti 107 heartz kramamlo acousto-optic ledhaa electro-optic modulatornu upayoginchi modulate cheyabadutundi. pampu mariyu probe kiranaalu appudu dicroic beamsplitternu upayoginchi tirigi kalapabadataayi mariyu namuunaapai drushti pettadaniki mundu point-bye-point image generation kosam galvanometric addaalanu upayoginchi skaan cheyabadataayi.
gurtinchadam.
probe modulation dwara utpatti cheyabadina signal asalu pump punjam kante chaalaa chinnadi, kabatti rendoo dicroic addaanni upayoginchi gurtinche maargamlo varnapatamgaa vaeru cheyabadataayi. probe signalnu aneka rakaala photo detectorlatho sekarinchavachhu, saadhaaranamgaa photodiode. appudu, pampu modulation frequenseeki tune cheyabadina lock-inn amplifiernu upayoginchi modulated signal vistarinchabadutundi.
dayta vislaeshanha.
hyperspectral dayta vislaeshanha maadirigaane, delay stock anie piluvabade pump-probe imaging daetaanu, antarleena paramaanu jaatula yokka paramaanu viruddhatatoe chitraanni pondadaaniki proses cheyali. pampu-probe daetaanu proses cheyadam aneka kaaranaala valla savaluga untundi-udaaharanaku, sankethaalu bipolar (saanukuula mariyu pratikuula), bahula-ghaataankam, mariyu rasayana vaataavaranamloo suukshma maarpula dwara gananeeyamgaa maarchavacchu. pump-probe dayta vislaeshanhaku pradhana paddhatulu bahula-ghaataanka amarika, pradhana bhagala vislaeshanha mariyu fazer vislaeshanha.
bahula-ghaataanka amarika.
bahula-ghaataanka amarikalo, samayam-parishkarinchabadina vakrarekhalu kshayam sthiraamkaalanu nirnayinchadaaniki ghaataanka kshayam namuunaatoe amarchabadi untaayi. eee paddhati suutigaa unnappatikee, idhi takkuva khachitatvanni kaligi untundi.
pradhana bhagala vislaeshanha.
pradhana bhagala vislaeshanha (pca) anedi pampu-probe dayta vislaeshanha kosam upayoginche prarambha paddhatulalo okati, endukante idhi saadhaaranamgaa hyperspectral dayta vislaeshanha kosam upayoginchabadutundi. pca daetaanu orthogonal bhagaluga vichchinnam chestundi. melanoma adhyayanaalalo, pradhana bhagalu vividha rakaala melanin nundi pondina sanketaalato manchi angeekaaraanni choopinchaayi. pca yokka prayojanam emitante, detaloni vyatyaasamlo ekkuva bhaagam unde pradhana bhaagaalanu matrame unchadam dwara sabdaanni tagginchavachhu. ayithe, pradhana bhagalu saadhaaranamgaa orthogonal kaani antarleena rasayana jaatula vaastava lakshanaalanu pratibimbimchavu. anduvalla, oka parimiti emitante, pratyaekamaina rasayana jaatula sankhyanu pca upayoginchi oohinchalemu.
fazer vislaeshanha.
fazer vislaeshanha saadhaaranamgaa fluorescence-life time imaging microscopy (flim) dayta vislaeshanha kosam upayoginchabadutundi mariyu pump-probe imaging dayta vislaeshanha kosam sweekarinchabadindi. sankethaalu icchina pounahpunyamlo forier parivartana yokka nijamaina mariyu oohaatmaka bhagaluga vichchinnam cheyabadataayi. nijamaina mariyu oohaatmaka bhaagaalanu okadaanikokati vyatiraekamgaa plaat cheyadam dwara, vibhina jeevitakaalaalato vividha chromophores pampineeni mapp cheyavachu. melanoma adhyayanaalalo, eee vidhanam melanin yokka vividha roopaala madhya thedaanu gurtinchagaladani mallee choopabadindi. fazer vislaeshanha yokka pradhana prayojanaallo okati, idhi parimaanaatmaka vislaeshanha kosam pcatho kalipi unna content yokka sahajamaina gunaatmaka, graphical veekshanhanu andistundi.
applications.
adhika-vegam mariyu adhika-sensitivity pump-probe imaging paddhatula abhivruddhi materials sains, biology mariyu art vanti aneka rangaalalo anuvartanaalanu praarambhinchindi.
materials sains.
graphane, nanocubes, nanovirelu mariyu vividha rakaala semikandaktarlu vanti suukshma padaardhaala adhyayanam mariyu lakshanaala kosam pump-probe imaging anuvainadi, endukante vaati pedda grahanaseelatalu conny balaheenamaina fluorescence kaaranamgaa. mukhyamgaa, yeka-godala carbon nanotubelanu vistrutamgaa adhyayanam chesi, submichrometer resolutiontho chitreekarinchaaru, ivi carrier dynamics, photophysical mariyu photochemical lakshanaala gurinchi vivaraalanu andistaayi.
jeevasaastram.
jeevasaastramlo pump-probe technic yokka modati anuvartanam dye-label sell yokka uddeepana udgaaraala inn vitro imaging. melanin eumelanin (godhuma/nalupu) mariyu pheomelanin (erupu/pasupu) yokka rendu pradhana roopaala madhya thedaanu gurtinchadaaniki melanin imaging kosam pampu-probe imaging ippudu vistrutamgaa upayoginchabadutundi. melanomalo, eumelanin gananeeyamgaa perugutundi. anduvalla, eumelanin mariyu pheomelanin pampineeni imaging cheyadam valla adhika sunnitatvamto nirapaayamaina gayalu mariyu melanomanu gurtinchadaaniki sahaayapadutundi.
kala.
kalaakrutiloo vistruta shreni varnapata shoshana lakshanaalatoo kuudina aneka varnadravyam untundi, ivi vaati rangunu nirnayistaayi. eee varnadravyam yokka vistruta varnapata lakshanaalu kaaranamgaa, mishramamlo oka nirdishta varnadravyam gurtinchadam kashtam. pump-probe imaging khachitamaina, adhika-resolution, molecular samacharanni andinchagaladu mariyu oke drushya rangunu kaligi unde varnadravyam madhya thedaanu gurtinchagaladu.

