
glucose auxidase

glucose auxidase enzyme (gokes kinwa godd) jyala notatin (eae. see. kramank 1.1.3.4) asehi mhanatat, haah eka oxidoriductase aahe joou glukojchya hydrogen peroxide aanee dii-glucono-dii. dii.-lactonemadhye auxidationla utprerit karato. heey enzyme burshi aanee kitkanchya vishisht prajatindware tayar kele jaate aanee jevha auxigen aanee glucose upasthit asatat tevha jivanurodhi kriyakalap darshavate.
glucose auxidescha vapar mothya pramanavar sharirachya dravpadarthanmadhye (vaidyakeey chachani), vanaspatijanya kachchya malamadhye aanee ann udhyogat mukt glukojchya nirdharanasathi keela jaato. jaivtantradnyanamadhye dekhil yache anek upayog aahet, visheshtah nanoe tantradnyanatil jaivasinvedkansaha jaivarsayanshastrasathiche enzyme parikshan. 1928 madhye detlev mullerne 'espergillus niger' pasoon teey pahilyanda vegale kele.
karya.
burshi aanee kitkanchya anek prajati glucose auxidesche sanshleshan karatat, jeey hydrogen peroxide tayar karate, jeey jivanunna marte.
penicillium notatumchya jivanurodhi sanvardhanatun kadhalelya notatinla moolatah penicillin aii asey naav denyat aale hote, parantu penicillinshi gondhal talhanyasathi tyache naav badalanyat aale. notatin heey penicillin bii aanee glucose auxidase sarkhe asalyache disun aale, jeey "pii" vyatirikt itar sachanmadhun kadhalele enzymes aahet. notam "; aata teey samanyatah glucose auxidase mhanoon olakhale jaate.
suruvatichya prayoganmadhye asey disun aale qee hydrogen peroxide tayar jhalyamule notatinmadhye (glukojchya upasthitit) 'inn vitro' jivanurodhi kriya disun yete. "inn vivo" chachanyanvarun asey disun aale qee, "streptococcus haemolitics", "staphylococcus aurius" kinwa salmonelapasun kruntakanche sanrakshn karnyasathi notatin prabhavi navhate aanee kahee matranmadhye uutinche gambhir nuksan jhaale.
madhmashi kamgaranchya hypopharengeal granthindware dekhil glucose auxidase tayar hoto aanee teey madhamadhye jamaa hote jithe teey naisargik snrakshak mhanoon karya karate. madhachya prushthbhagavaril gokes vatavaranatil oo2 qamee karoon hydrogen peroxide (etch2oo2) karate, joou pratijaivik adthala mhanoon kaam karato.
rachana.
gokes heey eka diameric prathine aahe, jyachi 3dii rachana spasht kelee gelee aahe. glucose jithe bandhala jaato tee sakriy jaaga khol khishat asate. peshinchya baher karya karananya anek prathinanpramanech, heey enzyme carbohydrate sakhalyanni jhakalele asate. gokes heey 160 keey. dii. aii. chya aanvik vajanasah glucose oxidising enzyme aahe. heey diameric glycoprotein aahe jyamadhye pratyeki 80 kilo dactor vajanache done upghatak asatat. sakriy thikani flavinadenine dynucleotide (phaid) prathinanchya kavchamadhye andaje 1.5 nm aat purale jaate aanee prarambhik electron sweekarak mhanoon karya karate.
yantrana.
ph 7 varr, glucose heey chakriy hemiacital swarupat 63.6% β-d-glucopyranose aanee 36.4% α-d-glucopyranose mhanoon dravanat astitvat asate, rekheey aanee furanose swarupache praman nagany asate. glucose auxidase visheshtah β-d-glucopyranose bandhala jaato aanee α-d-glucosever karya karat nahee. teey dravanatil serve glukojche auksidikaran karate kaaran α aanee β anomersmadhil santulan pratikriyemadhye sevan kele jaate tase β bajukade nele jaate.
glucose auxidase heey β-d-glukojche dii-glucono-1,5-lactonemadhye auxidation utprerit karate, jeey nantar gluconic aesidmadhye hydrolyse hote.
utprerak mhanoon kaam karnyasathi, goksala flavin edenine dynucleotide (phaid) yaa saha-utsechakachi aavashyakta asate. jaivik auxidation-reduction (redox) pratikriyanmadhye phaid haah eka samanya ghatak aahe. redox pratikriyanmadhye renupasoon electroncha laabh kinwa tota hoto. gokes-utprerit redox pratikriyemadhye, phaid prarambhik electron sweekarak mhanoon karya karate aanee teey phaid-madhye qamee hote. mug phaid-chey antim electron sweekarak, aanvik auxigen (oo2) dware auksidikaran kele jaate, jeey asey karoo shakate kaaran tyat qamee karanyachi kshamata jaast asate. tyanantar oo2 qamee houn hydrogen peroxide (etch2oo2) hoto.
anuprayog.
glukojche nirikshan.
glucose auxidescha vapar mothya pramanavar peroxidase pratikriyeshi jodala jaato, joou tayar jhalelya etch2oo2chee rangmapi drushtikonatun kalpana karato, nidanasathi seramadhye kinwa rakt plazmamadhye mukt glukojche nirdharan karnyasathi, swahaste kinwa svayanchalit prakriyesah spectrometric parikshanancha vapar karoon aanee agadi vaparachya binduvaril jalad parikshanansathi dekhil.
ashach prakarchya parikshananmule kinwan, jaivik abhikarmakatil glukojchya pataliche nirikshan karata yete aanee vanaspatijanya kachcha maal aanee annapadarthanmadhil glucose niyantrit karata yete. glucose auxidase chachanimadhye, glucose pratham auxidise kele jaate, glucose auxidesdware utprerit kele jaate, gluconet aanee hydrogen peroxide tayar karnyasathi. tyanantar hydrogen peroxidela oxidative paddhatine chromogens jodale jaate aanee rangeet sanyug tayar kele jaate jeey spectroscopic paddhatine mojale jaau shakate. udaharanarth, hydrogen peroxide aanee 4 amino-antipyrene (4-aap) aanee peroxidase upasthitit phenol miloon laal quinoimine rung tayar hoto joou 505 nmvar mojala jaau shakato. 505 nmvaril shoshan heey namunyatil glukojchya ekagratechya pramanat asate.
glukojche auksidikaran karnyasathi aanee glukojchya ekagratechya pramanat electronic pravah tayar karnyasathi aavashyak asalele electron ghenyasathi enzymatic glucose biosensor oo2aivaji electrodecha vapar karatat. madhumehachya rugnandware seeram glukojchya patalivar laksh thevanyasathi vaparlya jaanarya disposable glucose sensor pattyanmagil heey tantradnyan aahe.
ann sanrakshn.
utpadanat, goksacha vapar tyachya oxidising prabhavanmule eka yojak mhanoon keela jaatoh teey beckingmadhe majboot pithasathi soochit karate, bromet sarkhya auxidantschi jaaga ghete. avanchhit tapkiri rung aanee avanchhit chav talhanyasathi koradya andyachi puud yasarkhya pack kelyavar annatoon auxigen aanee glucose kadhoon taknyas madat karnyasathi ann snrakshak mhanoon dekhil yacha vapar keela jaato.
jakhamevar upchar.
jakhamechi kalji ghenari utpadane, jase qee "flaminal hydro", glucose auxidase aanee itar ghatak asalelya alginate hydrogelcha auxidation agent mhanoon vapar karatat.
clinical chachanya.
glucose auxidase aanee glucose misalananya pishavi-varr-zadap upkaranatun anunasik favaranichi samanya sardichya pratibandhasathi aanee upcharansathi 2016 madhye vaidyakeey chachanya ghenyat aalya aahet.