
anandi-pii. see. aar. parikshan

glal hydrolysis aanee bandhan adapterver avalambun asalele pii. see. aar. parikshan (anand-pii. see. aar. parikshan) hii dii. aine. emm. tii. zaza aanee dii. aine. emm. tii. zeb dii. aine. aii. methyltransfereses "dii novo" dii. aine. aii. methylation darmyan tayar jhalelya aar. (5 emm. see.) jee. aay. sites nishchit karanyachi naveen paddhat aahe. glade-pii. see. aar. parikshanasathi dii. aine. aii. chya bisulfite upcharanchi aavashyakta nasate.
yaa paddhatichi rachana visheshtah sambandhit unmethylated sthalanpeksha jaast pramanat manvi aanee sastan pranyanchya genomemadhe swarasya asalelya aar. see. jee. siteche methylation nirdharit karnyasathi karnyat aalee hotee. rakt aanee uutinchya vaidyakeey namunyanmadhun dii. aine. aii. tayar karnyasathi hii eka vaishishtyapurn paristhiti aahe.
glade-pii. see. aar. parikshan heey naveen prakarchya enzymesver aadharit aahe-sthal-vishisht methyl-nirdeshit dii. aine. aii.-endonucleases (emm. dii. dii. aine. aii. endonucleases). heey enzymes jaivarasayanik gunadharmanmadhil pratibandhatmak enzymessarkhech asatat aanee dii. aine. aii. purnapane vighatit karatat, parantu ulatya paddhatine karya karatatah teey keval methylated dii. aine. aii. vichchhedit karatat aanee unmethylated dii. aine. aii. ajibat vichchhedit karat naheet. sastan pranyanmadhil dii. aine. aii.-methyltransferesses dii. aine. emm. tii. 1, dii. aine. emm. tii. 3aii aanee dii. aine. emm. tii. 3bii, dii. aine. aii. methylation pratikriyes utprerit karatat.
heey sarvashrut aahe qee pravartakanchya niyamak kshetranmadhye aanee/kinwa vividh janukanmadhye pahilya exonmadhye see. pii. jee.-betanche hypermethylisation anekada turalak carcinogenesischya suruvatichya tappyat hote. yamule arbud peshinmadhil janukanchya abhivyaktiche niyaman qamee hote, tarr nirogi uutinmadhye sambandhit januke sakriy rahatat. asha prakare, asha epigenetic biomarkerscha shodh ghene heey nidan aanee purvasuchanatmak sadhananpaiki sarvat aashadayak sadhan aahe
dii. aine. emm. tii. 3aii aanee dii. aine. emm. tii. 3bii tharanchya vishishttechya abhyasatoon asey disun aale aahe qee donhi enzymes pramukhyane aar. see. jee. site olakhatat aanee 5 '-aar (5 emm. see.) jee. aay.-3'/3 '-vaay. jee. (5 emm. see.) aar-5' kram tayar karnyasathi antargat see. jee.-dynucleotidemadhye badal karatat. naveen enzymespacky eka gly site aar (5 emsee) gi olakhato aanee too todato. yaa adviteey thurr vishishtatemule, manvi aanee sastan pranyanchya dnmdhil 'dii novo' methylated sites olakhanyasathi gly heey eka soyiche sadhan aahe.anand-pii. see. aar. parikshanamadhye 3 sopya payya samavisht aaheth 
eka naliket parikshan kele jaate, sumare 2-3 taas lagatat aanee aar (5 emsee) gi sites dnachya anek pratee dekhil nirdharit karatat.