neutravidene

neutravidene prathine hii chikan avidinechi eka deglycosylated aavrutti aahe, jyache vastuman andaje 60,000 daltons aahe. karbodkanmadhe asalele padarth kadhoon takalyamule, lectinche bandhan nidan naan houu houu pataliparyant qamee hote, tarihi yaa kriyakalapasathi karbodkanmadhe aavashyak naslyamule biotineche bandhan tikavoon thewale jaate. avidinemadhye uchch paee asate parantu neutravidinmadhye jawaljawal-tatasth paee (ph. 6.3) asate, jeey nakaratmak-charge kelelya peshinchya prushthbhagashi kinwa dna/rnash gair-vishisht parasparsanvad qamee karate. neutravidinmadhye ajunahi lycine avashesh aahet jeey vyutpatti kinwa sanyugmanasathi upalabdh aahet.
swatah avidinpramanech, neutravidene haah biotineshee ("keey" dii = 10−15 meater) drudh sambandh asalela tetramer aahe. jaivarasayanik vaparat, streptavidine, jeey biotineshee agadi ghattapane bandhale jaate, teey neutravidines adlabadal karoon vaparale jaau shakate.
ghan aadharanvar sthir kelele avidine heey biotin-label kelele prathine kinwa nuclic acid renu pakadanyasathi shuddhikaran madhyam mhanoon dekhil vaparale jaate. udaharanarth, peshi prushthbhagavaril prathinanna visheshtah puttle-abhedya biotin abhikarmakasah label kele jaau shakate, nantar neutravidene aadhar vaparoon visheshtah tipale jaau shakate.
sandarbh.
vaay. hiller, jeey.emm. gershoni, eae.aaa. bayer, emm. vilchek jaiv rasayan. jeey., 248 (1987), pru. 167-171
eae.aaa. bayer, eff. daad mester, tii. kulik, emm. vilchek upkaran. jaivarasayan. biotech., 53 (1995), pru. 1-9
aaa.tii. martila aanee itar. februwari akshare 467 (2000), pru. 31-36 recombinant neutralite avidinh chikan avidinecha eka gair-glycosylated, amleey utparivartak joou biotinsathi uchch aakarshan aanee qamee gair-vishisht bandhan gunadharm darshavito

