
teey buffer

tii buffer heey aanvik jivashastrat, visheshtah dii. aine. aii., see. dii. aine. aii. kinwa aar. aine. aii. chaa samavesh asalelya prakriyanmadhye samanyatah vaparale jaanare buffer solution aahe. "tii" heey tyachya ghatakanpasun tayar kele gele aaheh triss, eka samanya ph buffer aanee edda, eka renu joou emm. jee. 2 + sarkhya catayanla chillet karato. dii. aine. aii. kinwa aar. aine. aii. vidravya karane, tasech tyache ksharanapasun sanrakshn karane haah tii. buffercha uddesh aahe.
pakakruti.
1x te buffer banavanyasathi eka vaishishtyapurn pakakruti aaheh
tii bufferla tii10eae1 buffer asehi mhanatat, jeey "tii teena eae vann buffer" mhanoon vachale jaau shakate.
tii10eae1 bufferche 100 miilii dravan tayar karnyasathi, 1 miilii trius bases (ph 10-11) aanee 0.2 miilii edda (0.5 meater) yanche 1 miilii misaloon 100 miliparyantchya duheri distilled panyane tayar kele jaate. ph 8 paryant qamee karnyasathi uchch molarity etch. see. ell. chey microlitor praman joda.
1980 chya dashkatil nuclease abhyasachya aadhare, ph samanyatah rnsathi 7.5 aanee dnsathi 8.0 madhye samayojit kele jaate. yaa ph mulyanvar sambandhit dna aanee rna nucleases qamee sakriy asavet asey manale jaate, parantu dna aanee rna yaa donhichya sathavanukisathi ph 8 surakshitpane vaparale jaau shakate.
yaa enzymela aavashyak asalelya dhatuchya catayanshi bandhun edda pudhe denace nishkriy karate.
genomic aanee plasmid dna alpakalin vaparasathi 4°seey. (39.2°pha.) varr tii buffermadhye kinwa dirghakalin sathavanukisathi-20°seey. (-4°pha.) teey-80°seey. (-112°pha.) varr sathavale jaau shakatat. varanvar gothvan-vitalanyachi chakram talhali pahijet.
qamee teey kinwa qamee edda.
tii bufferche karya heey emm. jee. 2 + sarkhya dhatuchya catayanchya chillettingwar aadharit aahe. samasya ashee aahe qee pii. see. aar. polymerasela karya karnyasathi emm. jee. 2 + chee dekhil aavashyakta asate, mhanoon jarr eddache praman khoop jaast asel tarr tyacha pii. see. aar. varr parinam houu shakato. 10 putt qamee pramanat edda asaleli tii bufferchi eka aavrutti aahe jee nyayvaidhyak ess. aar. kitsathi varanvar vaparali jaate. multiplex emplifications sanchanchya vaparamule, pratimemadhe upasthit asalelya mii. gra. 2 + madhye hastakshep houu naye mhanoon namunyat edache praman qamee asane aavashyak aahe. jarr namuna patal karnyasathi niyamit tii buffercha vapar keela gela tarr dii. aine. aii. profilemadhye asantulan disun yeil, tarr loow tii. madhye adhik changle santulan asel. loow tii buffer kinwa tii loow edda buffer 10 miimii triss-etch. see. ell. (ph. 8.0) + 0.1 miimii eddane banalela asato.
sandarbh.
ph7.4 tii buffer = 100 miimii/ell triss (ph7.4) + 10 miimii/ell edda (ph8.0)
aanvik cloning eka prayogshala niyamavali