
inn situ electron microscopy

inn seatoo electron microscopy heey eka tapas tantra aahe jithe electron microscopecha vapar ekhadya uttejkala namunyacha pratisad pratyaksh velet pahanyasathi keela jaato. electron sukshmadarshakamadhye namunyachi pratima kadhanyasathi vaparlya jaanarya electronchya uchch-urja kirananchya swarupamule, sukshmadarshakanni dirghkal nirikshan kele aahe qee electron kiranadvare namune niyamitpane badalale jaatat kinwa kharab hotat. 1960 chya dashakapasun, aanee pareshan electron sukshmadarshak (tii. emm. ess.) vaparoon, shastradnyanni namuna namuna kakshat asatana samgrimadhye badal karnyasathi aanee prerit nukasanichya velenusar pratima ghenyasathi jaanunbujun prayatna kele.
1960 chya dashakat dekhil, temscha vapar karoon samagri shastradnyanni electron-pardarshak dhatuchya namunyanchya electron bimdware honanya vikiranas milananya pratisadacha abhyas karnyas suruvat kelee. vimanchalan aanee antaral uddanadarmyan dhatuchya thakvyabaddal adhik samajoon ghenyasathi heey kele gele hote. uchch pravegak vidyutdab asalelya upkarnanvar prayog kele gele; aadhunik tapmanasah upalabdh asalelya up-nanometer resolutionchya tulanet pratimeche resolution qamee hote. 
1960 chya dashakapasun electron sukshmadarshakiy sudharananmadhye avkashiy sankalp vadhavnyavar laksh kendrit karnyat aale. yasathi sampoorn imaging platformsathi, parantu visheshtah namuna tappyachya aaspaschya kshetrasathi vadhiv sthirata aavashyak hotee. charge-coupled device camerey vaparoon sudharit pratima-capture pranali aanee namuna tappyatil pragati aanee uchch resolutionmule vishesh dharkanmadhil namunyanna uttejan denyasathi samarpit pranali tayar karnyat aanee namunyanchya pratisadanchi anek chaukati kinwa video capture karnyat madat jhaali. 
samgrichya namunyanvyatirikt, inn seatoo electron microscopy jaivik namunyanvar kelee jaate aanee yantrik, rasayanik, aushnik aanee vidyut pratisadancha samavesh asalele prayog karnyasathi vaparali jaate. suruvatichya prayoganmadhye bahutek vela tempcha vapar keela jaato, kaaran pratima ekach chaukatit tipali jaate, tarr scanning electron sukshmadarshikela uttejana lagoo kartana namunyachya palikade halavane kinwa scan karane aavashyak asate, jyamule namuna badalato. 
mool electron sukshmadarshanat maryadit asalelya suruvatichya samasyanmadhye serve pramananvar yantrik compan (sukshmadarshakapasun namunyaparyant) aanee aushnik aanee vidyut hastakshep, visheshtah namuna dharkamadhye samavisht hote. yaa serve samasyansathi jalad capture velechi aavashyakta hotee. tathapi, jalad capture vel qamee signal-too-noise gunottar asaleli pratima tayar karate, pratimeche resolution maryadit karate aanee prayog karnyasathi upalabdh asalelya veleche praman dekhil maryadit karate.