mahadhamani-gonad-mesonephros

mahadhamani-gonad-mesonephros (aii. jee. emm.) haah bhrun mesodermcha eka pradesh aahe joou kombadi, undeer aanee manvi garbhanmadhil parra-mahadhamani splanknopluramdhun garbh vikasadarmyan vikasit hoto. praudh vikiranit praptakartyanchya dirghakalin bahu-vanshiy punaravartan karnyas saksham asalelya pahilya praudh nishchit raktaranjit moolpeshi, 'endothelial-too-hematopoetic transition' (eae. etch. tii.) mhanoon olakhalya olakhalya endothelial transdiferentiation prakriyedvare, garbh prushthiy mahadhmanichya ventral endothelial bhintitun udbhavtat. mausachya bhrunamadhye, heey agadi pahile etch. ess. see. tyanchya tyanchya. eff. pii. pii, see. dii. 31, see. dii. 34, see. kiit., see. dii. 27, see. dii. 41, gaata 2, runex 1, notch 1 aanee bii. emm. pii. yaa abhivyaktidvare darshavile jaatat. 
mahadhamani-gonad-mesonephros (aii. jee. emm.) pradesh heey garbhatil manav, under aanee pakshi aanee zebrafish sarkhya sastan pranyansarkhya prushthavanshiy nasalelya pranyanmadhye olakhalya olakhalya splanknopleura mesodermpasoon tayar kelele kshetra aahe. tyat prushthiy mahadhamani, jananendriyachya kadya aanee mesonephros asatat aanee notocord aanee sharirik mesodermchya darmyan sthit asatat, jeey nabhipasoon garbhachya agrabhagachya anganchya kaliparyant pasarlele asatat. aii. jee. emm. pradesh garbhachya vikasat mahattvachi bhoomika bajavato, heey nishchit raktastravasathiche pahile swayatt antargarbhashayi thikan aahe. nishchit raktaranjit peshi hematopoeatic mool peshi tayar karatat jyaa kiranotsargi praptakartyanmadhye kramikpane pratyaropit kelyavar 'swayam-nutanikaran' karanyachi kshamata asatat aanee praudh raktaranjit peshi padanukramachya konatyahi raktpeshinchya vanshamadhye farak karatat. haemogenic endothelium mhanoon olakhalya olakhalya prushthiy mahadhmanichya (aii. jee. emm. pradeshatil) majlyavaril vishesh endothelial peshi hematopoetic stame peshinmadhye bhinn hotat.
garbhachya vikasat.
aii. jee. emm. kshetra heey garbhachya mesoderm tharatoon tayar kele jaate. organogenesischya darmyan (manvi garbhatil chauthya aathvadyachya aaspas), mesodermcha aatadyacha bhag, splanknopleura, prushthiy mahadhamani, jananendriyachya kadya aanee mesonephros yancha samavesh asalelya vishisht sanrachananmadhye rupantarit hoto. garbhachya vikasadarmyan kahee kalasathi, prushthiy mahadhamani hematopoetic stame peshi tayar karate, jyaa akheris yakrutavar vasahat nirman kartil aanee praudhanmadhye serve paripakv rakt vanshavalinna janm deteel. janmanantar, prushthiy mahadhamani avarohi mahadhamani banate, tarr jananendriyachya kadya gonads tayar karatat. mesonephros nephron aanee mutrapindanchya itar sambandhit sanrachana tayar karatat.
zenopus levisasarkhya sastan pranyansarkhya prushthavanshiy nasalelya pranyanmadhye aii. jee. emm. pradeshachya nirmitiche sarvottam varnan kele gele aahe. jatharprajvalanantar lagechach, standharkanmadhil splanknopleura mesodermshee samaan asalelya dorsolateral platemadhil peshi, prushthiy mahadhamani tayar karnyasathi notocordchya khalee, madhyareshekade sthalantarit hotat aanee parshvikpane cardinal vence aanee nephric duct tayar hotat.
karya.
mahadhamani gonad mesonephros pradeshache sarvat mahattvache karya mhanje nishchit raktastravamadhye tyachi bhoomika. nishchit raktastrav hii garbhatil raktastravanchi dusari laat aahe aanee praudh raktastrav pranalitil serve raktastrav moolpeshi nirman karate. mahadhamani gonad mesonephros pradeshat bahuvidh hematopoetic vasahat-nirman karnare ekak-pleeha (see. eff. yuu.-ess.) purvaj peshi aanee pluripotential dirghakalin punarvapar punarvapar hematopoetic stame peshi (ell. tii. aar.-etch. ess. see.) aadhaltat. extra-embryonic hematopoetic site asalelya pivalya pivalya pishavichya ulat, mahadhamani gonad mesonephros pradeshat see. eff. yuu.-ess. chee sankhya khoop jaast hotee. lonichi pishavi aanee garbhachya yakrutapeksha thodya lavkar mahadhamani gonad mesonephros pradeshat dekhil ell. tii. aar.-etch. ess. see. kriyakalap aadhalala. asha prakare yaa pradeshatil nishchit raktastrav shakti soochit karate. shivay, undaranchya garbhatil aii. jee. emm. chey vegale avyav sanwardhan, pivalya pivalya pishavicha kinwa yakrutacha prabhav naan padata, swayattapane hematopoetic stame seela kriyakalap suru karoo shakate. 10 divasanni coitus nantar (aaa.pii.see.) mahadhamani gonad mesonephros pradesh nishchit raktpesheey moolpeshi kriyakalap suru karnyas aanee vistarnyas saksham hota, tarr pivalya pivalya pishavit 11 dii paryant konatihi raktpesheey kriyakalap disun aalee nahee.pii.see. manvi bhrunanmadhyehi asech aahe, jithe teey mahadhamani gonad mesonephros pradeshat 27 vya divashi pahilyanda aadhaltat, 35 vya divashi vegane vistartat, nantar 40 vya divashi adrushya hotat. heey "gayab hone" yaa hematopoetic stame peshinchya garbhachya yakrutat sthalantarit honyashi sambandhit aahe, jithe teey nantarche hematopoisische thikan banate.
histology.
prushthiy mahadhmanimadhye endothelial thurr aanee antarnihit stromal thurr asato. hematogenic endothelium navachi aanakhi eka peshi sankhya dekhil aahe, jee hematopoetic stame peshi tayar karnyasathi endothelial tharatoon tayar hote.
endothelial peshi.
endothelial peshi serve raktavahinyanchya lumenla eka squamous endothelial thurr mhanoon rekhatatat. yaa peshi ghatt jodyandware ekmekanshi sampark sadhatat. aii. jee. emm. madhye, endothelial peshi prushthiy mahadhmanichya lumenla rekha lavtat. endothelial peshincha eka vishesh upasanch, haemogenic endotheliummadhe hematopoetic stame peshinmadhye farak karanyachi kshamata aahe.
haemogenic endothelium.
prushthiy mahadhmanichya ventral endotheliumla ghatt chikatoon asalelya hematopoetic stame cells (etch. ess. see.) aadhalun aalya. hematogenic endotheliumpasoon yaa peshi udbhavtat, jeey hematopoetic aanee endothelial vanshanchya purvavarti aahet. yethech etch. ess. see. heey dorsa mahadhmanichya endothelial astarapasun vegale hote. endothelial peshinsathi eka vishisht chinhak asalele vhee-caderine heey mahadhamani endotheliumchya luminal bajula aadhalate. prushthiy mahadhmanichya bhintivar samoohit jhalelya peshi dekhil vhee-cadrine tasech see. dii. 34, eka samanya hematopoetic aanee endothelial marker; aanee see. dii. 45, hematopoetic peshinvar upasthit asalele marker dekhil vyakt karatat. jevha yaa vishesh endothelial peshi 'inn vitro' madhye sanvardhit kelya gelya, tevha tyaa hematopoetic moolchya peshinpeksha uchch darane hematopoetic stame peshi tayar karnyas saksham hotya. asha prakare donhi vanshatil peshi prushthbhagachya chinhakanchi saha-abhivyakti asey soochit karate qee hematopoetic stame peshi aii. jee. emm. madheel prushthiy mahadhmanichya endothelial peshinpeksha bhinn asatat.
jiwant zebrafish bhrunanchya velechya ulatya imagingne hematogenic endotheliumche hematopoetic stame peshinmadhye vegale honyache drushya pradan kele aahe. fertilisationanter sumare 30 tasanpasoon, dii. etch. ess. see. chya pahilya pradarshanachya kahee taas aadhi, mahadhmanichya majlyavaril anek endothelial peshi sankuchit houu lagatat aanee up-mahadhamani jagekade vakoo lagatat, jee sahasa 1 teey 2 taas tikatat. tyanantar yaa peshi madhyavarti akshasah aanakhi aakunchan pavtat, tyachya done bajukadil endothelial shejari ekatra aanatat aanee tyanchyashi tyacha sampark sodatat. udayas aaleli peshi eka golakar aakarvidnyan gruheet dharate aanee patrachya akshavar pravas karnyasathi rostral aanee coddle endothelial peshinshi majboot sampark rakhate. electron sukshmadarshakachya pratima darshavtat qee yaa peshi ghatt jodyandware sampark rakhatat. ekada heey sampark virghalale qee, peshi, tyachya epical-bases dhruviyatemule, up mahadhamani jaaget jaate aanee parinami itar hematopoetic avyavanna vasahat karate.
raktaranjit moolpeshicha vikas.
etch. ess. see. chya aii. jee. emm. utpadanat, haemogenic endothelial peshi mahattvachi bhoomika bajavtat asey manale jaate. haemogenic endothelial peshi yaa vishisht endothelial peshi aahet jyaa ekach velee hematopoetic aanee endothelial marker donhi vyakt karatat. heey haemogenic endothelial peshi nantar sakriy hotat, tyanche bandhane jawalchya endothelial peshinshi sodatat aanee 'budding' mhanoon olakhalya jaanarya prakriyet parisancharanat pravesh karatat. heey undaranchya vikasit vikasit bhrunat eae. 9.5 varr ghadate. yethoon haemogenic endothelial peshi etch. ess. see. madhye vikasit hotat. tathapi, haemogenic endothelial peshi sakriyatemadhye samavisht asalela achook sanket marg adnyat aahe, parantu nitrick auxide (kramank), notch 1 aanee runex 1 yasah anek sanket renu samavisht kele gele aahet. 
aii. jee. emm. haemogenic endothelial peshi sakriyakaranat samavisht asalelya sanket marganmadhye khaleel goshtincha samavesh hotoh
hotoh.
karel (jyala aii. emm. ell. 1 asehi mhanatat) haah eka pratilekhan ghatak aahe joou aii. jee. emm. madheel haemogenic endothelial peshinchya nirmitimadhye aanee sakriyatemadhye mothya pramanat guntalela aahe. runex 1 baad jhalelya abhyasanmadhye eae12 varr bhrun ghatak honyapurvi garbhachya serve uutinmadhil nishchit raktaranjit kriyakalap purnapane kadhoon takalyache disun aale aahe. runex 1 baad jhalelya khelanmule agmadhe aakarshastriy badal dekhil hotat, jyat messenkimal peshinchi jaast gardi hote. messenkimal peshi endothelial peshinmadhye bhinn hott asatana, runex 1 chya anupasthitimule messenkimal peshinchya haemogenic endothelial peshinmadhye bhinn karnyachya kshamatevar parinam houu shakato. heey messenkimal peshi sankhyetil vaadh aanee itar raktaranjit rog chinhakansathi sakaratmak peshincha vishisht abhav spasht karel. karel heey haemogenic endotheliumchya sakriyatemadhye dekhil samavisht kele gele aahe. sashart nockoutscha vapar karoon asey darshavile gele qee aii. jee. emm. haemogenic endothelial peshinmadhil runex 1 abhivyakti kadhoon takalyane etch. ess. see. chey utpadan rokhale gele. tyach prayoganni asehi dakhavun dile qee ekada etch. ess. see. tayar jhaale qee, niyantrananchya tulanet etch. ess. see. kriyakalapanmadhye konatahi vichalan naan karanyachi aavashyakta runex 1 laa rahili nahee. yavyatirikt, jevha runex 1 nockoutmadheel aii. jee. emm. peshi 'inn vitro' madhoon 'overexpress runex 1' madhye retroviral hastantaran karatat, tevha tyanna vachavale jaau shakate aanee teey nishchit raktaranjit peshi tayar karoo shakatat. heey soochit karate qee raktajankiy peshi sakriy karnyasathi aanee messenkimal peshinmadhun tyachya utpadanasathi signal margat runex 1 mahattvapoorn bhoomika bajavate.
nitrick auxide.
nitrick auxide signalling dekhil raktajankiy endothelial peshi utpadan aanee sakriyakaranat bhoomika bajavate asey darshavile gele aahe, bahudha runex 1 chya abhivyaktiche niyaman karoon. rakt pravahatoon yenara nivval taana raktavahinyanmadhil yantrik receptorsna nahee tayar karnyasathi sakriy karato, jyamule konatehi utpadan parisancharan avalambun nasate. heey aine. see. ex. 1 nockoutsmadhe disun yete, jithe hrudayache thoke vikasit karnyat apayash aanee parinami parisancharan naan jhalyane dhaav dhaav niyaman qamee hote aanee aii. jee. emm. madhye raktaranjit kriyakalap hott nahee. jevha aine. see. ex. 1 nockoutsla bahya srot nahee mhanoon puravale jaate, tevha aii. jee. emm. madheel raktastrav kriyakalap jawaljawal jangali-prakarchya patalivar parat yeto. heey keval raktabharachi upasthiti navhe tarr raktavahinya niyantrit karnara mukhya ghatak mhanoon konatehi sanket vegale karat nahee. tathapi, 'nahee' laa laa abhivyaktishi jodanara sanketchinh pravah adyap spasht jhalela nahee. icam-1 yaa peshi sanlagnatechya renunchya abhivyaktiche niyaman karoon endothelial peshinchya halchalinvar niyantran thevanyasathi konatehi sanket dekhil darshavile gele naheet. yamule raktavahinyanmadhil haemogenic endothelial peshinchya paribhramanat vaadh honyat tyacha samavesh asanyachi shakyata aahe. raktajankiy endothelial peshi sakriyatesathi dekhil runex 1 mahattvapoorn asalyane, heey shakya aahe qee konatehi yaa donhi khalchya prabhavanche niyaman karat nahee.
notch signalling.
notch 1 heey aanakhi eka prathine aahe jeey etch. ess. see. utpadanasathi signal margat samavisht kele gele aahe. notch 1 nockoutsmadhe pivalya pivalya pishavit samanya raktastrav disun yeto, parantu aii. jee. emm. madhye konatehi etch. ess. see. tayar karnyat ayashasvi hotat. prayoganmadhye asey disun aale aahe qee qamee jhalelya notch 1 abhivyakticha runex 1 chya abhivyaktivar dekhil parinam hoto, parinami tyache niyaman qamee hote. pudhil prayoganmadhye jyamadhye notch 1 atiprakatit aahe, teey aii. jee. emm. chya endotheliummadhe vikasit vikasit nishchit raktastravi peshinche mothe samooh darshavtat. runex 1 abhivyakti hii raktpesheey peshi utpadanachya pramanat asalyane, heey parinam soochit karatat qee notch 1 dekhil runex 1 niyantrit karnyat sameel aahe.

