bahuroopi dii. aine. aii. chey yadruchchik pravardhan

yadruchchik pravardhit bahuroopi dna (rapid), jyala "jalad" asey uchcharale jaate, haah eka prakarcha polymerase sakhali pratikriya (pii. see. aar.) aahe, parantu pravardhit kelele dnahe vibhag yadruchchik aahet. rapp karat asalela shastradnya anek aniyantrit, lahan primer tayar karato (10-12) 
nucleotides), nantar genomic dii. aine. aii. chya mothya namunyacha vapar karoon pii. see. aar. saha pudhe jaate, ashee aasha qee tukade vadhatil. parinami tayar jhalelya namunyanche nirakaran karoon, rapp pratikriyetun ardh-adviteey profile midavata yete.
lakshyit genomechya dii. aine. aii. anukramache dnyan aavashyak nahee, kaaran primer yaa kramane kuthetari bandhale jaatil, parantu nemke kuthe heey nishchit nahee. yamule vaidnyanik samudayache laksh naan gelelya jaivik pranalinchya dii. aine. aii. chee tulana karnyasathi kinwa tulanene qamee dii. aine. aii. anukramanchi tulana kelya kelya pranalimadhye (see. dii. aine. aii. detabank tayar karnyasathi tee yogya nahee) hii paddhat lokpriy hote. kaaran teey mothya, akhand dii. aine. aii. template kramavar avalambun aahe, tyamule adhogati jhalelya dii. aine. aii. namunyanchya vaparat tyala kahee maryada aahet. tyachi nirakaran shakti lakshit, prajati-vishisht dii. aine. aii. tulana paddhatinpeksha khoopach qamee aahe, jase qee lahan tandam punaravrutti. alikadchya varshant, rapedcha vapar vividh vanaspati aanee prani prajatinchya vanshjanan vaishishtyasathi aanee shodhanyasathi keela jaat aahe.
parichay.
rapid marker heey decamer (10 nucleotides lamb) dii. aine. aii. chey tukade asatat, jeey janukeey dii. aine. aii. chya yadruchchik bhaganchya pii. see. aar. pravardhanatun aniyantrit nucleotide anukramachya ekach primers tayar kele jaatat aanee jeey punarutpadak paddhatine nasale taree anuvanshikdrushtya bhinn vyaktinmadhye farak karnyas saksham asatat.
yadruchchik primer vaparoon vyaktichya anuvanshik vividhateche vishleshan karnyasathi yacha vapar keela jaato. prayogacha punarutpadaktetil samasyanmule, anek vaidnyanik niyatkalike aata keval rapver aadharit prayog swikarat naheet.
rapdeela pravardhanasathi fakt eka primerchi aavashyakta asate.
teey kase karya karate.
pii. see. aar. neey vaadh kelyanantar, jail electrophoresisasathi namune jailmadhye (ektar egroj kinwa polyacrylamide) bharale jaatat. yadruchchik pravardhanadvare tayar kelele vegvegale aakar namuna strotapramane punha karata yenyajogya paddhatine jailchya bajoone vegale hotil. yamule eka vegale dii. aine. aii. fingerprint tayar hote.
paramparik pii. see. aar. vishleshanachya ulat, rappedla lakshyit jivachya dii. aine. aii. anukramache konatehi vishisht dnyan aavashyak nasateh samaan 10-meterche primer, primerchya anukramala purak asalelya sthananvar avalambun, dii. aine. aii. chya eka bhagala vadhavtil kinwa vadhavtil nahee. udaharanarth, jarr primer khoop durvar kinwa primerchya 3 'tokanna tond dett naslyas konatahi tukada tayar hott nahee. tyamule, jarr purvi primerla purak asalelya thikani template dii. aine. aii. madhye utparivartan jhaale asel, tarr pii. see. aar. utpadan tayar kele jaanar nahee, parinami jelvar pravardhit dii. aine. aii. vibhagancha vegala namuna tayar hoto.

