lacritin

lacritin heey 12.3 kiloda glycoprotein aahe jeey lackert janukadware manavanmadhye encode kelele aahe. ashru prathinanchya srawala uttejan uttejan ghatkansathi lacritincha shodh padadyavarun samore aala. lacritin heey ashru aanee lalemadhye aadhalanare sravit prathin aahe. lacritin ashru srav, upakala peshincha prasar aanee astitwala dekhil protsahan dete aanee asha prakare parpatalichi jakham bharoon kadhanare lacritin heey peshichya astitva kriyakalapasaha eka bahuuddeshiy abhiyojak mitogen aahe. lacritinche naisargik kinwa jivanu vichhedan see-terminal tukada sodate joou jivanurodhi aahe.
bahutek lacritin heey lacrimal granthidvare tayar kele jaate, jyat wolferingchya sahayak lacrimal granthicha samavesh aahe. kahee lacritin meibomian granthidvare aanee netrashleshmala aanee parpatalachya upakala peshindware tayar hote. yaa upakalanat ektritpane lacrimal functional unitcha (ell. eff. yuu.) barachsa bhag asato. korade dolhe haah ell. eff. yuu. chaa sarvat samanya rog aahe. vadhatya abhyasatoon asey soochit hote qee contact lensshee sambandhit koradya dolhyasah koradya dolhyanmadhye lacritinche bhinn pramanat niyaman kele jaau shakate. sasachya purv chikitsalayin abhyasat samyik lacritin fadnyala protsahan dete. koradya dolhyachya aayar nockout maus modelmadhye (manvi sjogren syndromsarkhe manale jaate), samyik lacritin pyelocarpin-prerit fadane punarsthapit karate, mothya pramanat lisaminecha hirwa daag door karate aanee lacrimal granthitil dahak kendrancha aakar qamee karate.
lacritin peshinche lakshya heparan sulfate proteoglycan syndican-1 (ess. dii. see. 1) yaa peshinchya prushthbhagavar avalambun asate. bandhan heey enzyme-niyantrit 'auph-aun' swichcha vapar karate jyamadhye sakriy epithelial heparanej (hpse) heparan sulfateche vibhajan karoon syndecan-1 chya mukhya prathinanchya aine-terminal pradeshatil bandhan sthal ughad karate. jee-prathine-jodalele receptor (jee. pii. see. aar.) nantar jodale gelyasarkhe disate. jarr paristhiti prasarasathi yogya asel tarr lakshyit peshi enfat aanee emm. tii. aar. laa kinwa tanavachya paristhitit aii. keey. tii. aanee foxo 3 laa sanket detat.
rachana.
aine-terminal signal peptidechya vichchedananantar lacritinemadhye 119 amino acid asatat aanee mukhytah see-terminal ardhya bhagat anek anumanit alfa helices pradarshit karatat. yapaiki done see-terminalchi pushti golakar dvibhajana jhaali aahe. sarvat see-terminal alfa helics heey amphipathic aahe, jyat viruddha viruddha hydrophobic aanee hydrophilic avashesh aahet. hydrophobic chehara haah eka mahattvacha syndecan-1 bandhan ghatak aahe. pandar (naisargikaritya avyavasthit pradeshancha andaj lavnara) assa andaj vartavato qee see-terminal aanee aine-terminal ardhe anukrame 'krambaddha' aanee 'avyavasthit' aahet. 11-12 andajit oo-glycosylation sites aine-terminal ardhya bhagat loksankhya vadhavtat. see-terminal amphipathic alfa helics heey lacritinchya ekmev aine-glycosylation saitache thikan dekhil aahe. climatic droplet keratopathy' madhye heey thikan glycosylated nahee. eae madhye lacritin punha ektritpane tayar hote. kolai (glycosylation nahee) aanee ashrunmadhil lacritin (glycosylated) ess. dii. ess.-prushthanusar ~ 18 aanee ~ 25 keey. dii. aii. chya sambandhit halchalinsah aakarat bhinn asatat. 12. 3 keey. dii. aii. chya prathinanchya gabhachya aanvik vajanasah, lacritinchya amphipathic alfa helisesdware gatishilata anshatah mandavane shakya aahe. lacritinchya mukhya prathinancha anumanit pii "aay" 5 aahe.
lacritin heey uutinchya transglutaminezdwar paraspar jodanichya adheen aahe, jyamule diamer aanee treamers lacritin multimer tayar hotat. 1 minitanchya aat cross linking suru kele jaate, jyasathi 0.9 nm lacritinchi aavashyakta asate. manvi ashrunmadhye andaje ootee transglutaminezchi 0.6 micro moller patali cross linkingla protsahan denyasathi pureshi aahe. cross linkingmadhye datyanche lycine 82 aanee 85 aanee swikaranare glutamine 106 samavisht asatat. glutamine 106 heey syndican-1 chya aine-terminusla bandhanyasathi jababdar asalelya see-terminsjaval amphipathic alfa heliksmadhye rahate. tyanusar, cross linked lacritin syndican-1 laa kharabaritya bandhate aanee nishkriy asate.
nebank istchya aakadevarivarun, esvyumadhye lacritinche anek prakar aadhalale aahet. lacritin-bii (11.1 keey. dii. aii.; pii "aay" 5.3) madhye civexilt yaa kramacha abhav aahe. lacritin-see (10.7 keey. dii. aii.; pii "aay" 4.6) eka naveen see-terminus darshavite joou syndecan-1 laa bandhanyat aksham asava aanee peshi tikoon rahnyachya kriyakalapancha abhav asava.
splies prakar heey proteophorms aahet. proteoformmadhye lacritinchya proteolytical prakriya kelelya prakarancha samavesh hoto. varchya khalchya vastuman vishisht anukramanmadhun asey disun aale qee manvi ashrunmadhye paach aine-aanee bavis bhinn see-terminal lacritin-eka proteophorm asatat. kahijan lacritinchya see-terminsmadhun 'aine-104', 'aine-94' aanee 'aine-94/see-6' heey jaiv sakriy lacritin krutrim peptides andaje manatat. protease avarodhak abhyas asey suchavatat qee see-terminal proteoformmadhye lacritinchya prakriyesathi cathepsin bii, calpain, alenil emino peptidase, arginyl aminopeptidase, emm. emm. pii. 9, emm. emm. pii. 10, cathepsin jee, plazma calicrine, plasmin, thrombin aanee tripsin yasah vividh ashru proteasechi aavashyakta asate. abadhit lacritinsarkhe see-terminal proteophorm, koradya dolhyanchya ashrunmadhye nivadak pramanat qamee asatat.
peshi lakshyikaran.
lacritin heey fibroblastic, glioma kinwa lymphoblastic peshinna navhe tarr upakala peshinchya pratibandhit gatala (manvi parpatliy upkalasah) lakshya karate. peshi prushthbhagavaril proteoglycan syndecan-1 anshatah jababdar aahe.
lacritin-pratisadatmak peshitil biotinilated peshi prushthbhagavaril prathine sharirik mithachya paristhitit lacritins ubavali gelee. lacritinla lacritinla vastunche kramvarit maas spectrometridware kele gele. kahee bandhalele. sarvat pramukh syndecan-1 (ess. dii. see. 1) hote. pushtiksham pull-daun parikshananmadhye, bandhan heey kutumbatil sadasyansah syndecan-2 kinwa syndecan-4 saha samayik kele gele navhate, jeey soochit karate qee prathinancha gabha (aanee nakaratmak charge asaleli heparan sulfate side-cheen nahee) heey bandhan karnyache mukhya thikan hote. pudhil vishleshanane hii jaaga syndecan-1 chya aine-terminal 51 amino esidparyant sankuchit kelee aanee tyanantar vegvegalya prajatinmadhun syndecan-1 madhye sanrakshit kelelya aine-terminal sequence gagleparyant sankuchit kelee. gaggle lacritinchya see-terminal amphipathic alfa helixchya alfa helicityla protsahan dete aanee bahudha hydrophobic hydrophobic bandhate. syndecan-1 tyachya lamb heparan sulfate bajuchya sakhalyandvare anek vadhichya ghatakanchi bandhani karate. tarihi, lamb heparan sulfate sakhali lacritinchya bandhanimadhye hastakshep karatat. syndecans nehmich heparan sulfetne sushobhit kele jaat asalyane, yacha arth heparan sulfate anshatah kinwa purnapane vighatit karnyasathi hapernays upalabdh asane aavashyak aahe, jyamule lacritinla bandhata yete. kharey tarr, sirna qamee jhalyanantar heparanejachi kamatarata asalelya peshinmadhye konatehi bandhan aadhalale nahee. bahya heparanej kinwa heparaitinejmadhye spike karoon bandhan punarsanchayit kele gele. asha prakare, heparanej lacritinche karya 'aun-switch' mhanoon niyantrit karate. heparanez-cleved heparan sulfatevaril 3-oo sulfated gut (jeey bahudha lacritinchya see-terminal amphipathic alfa helixchya catanic fesashi sanvad sadhatat) aanee aine-terminal chaundroitin sulfate sakhali (bahudha catanic fesashi dekhil bandhate) bandhanat yogdan detana disatat. lacritinchya point mutagenesismule bandhansthalachi jaaga sankuchit jhaali aahe. heey naveen heparanej yantrana pahilya najaret dolhyanchya aarogyasathi kharab asalyache disate kaaran parpatliy stromamadhe aakraman naan lymphocytespasoon heparanejache prakashan dahak asate. tarihi heparanej heey parpatli upakalakache samanya srav utpadan aahe.
lacritin-aadharit mitogenesis heey pertusis toxindware pratibandhit kele jaate. yacha arth assa aahe qee lacritin lakshyankit vishishtatecha aanakhi eka mahattvacha ghatak mhanje jee-prathine-jodalela receptor joou bahudha ess. dii. see. 1 saha lakshyankit sankul tayar karel. jee-prathine jodalelya receptorcha sahbhag lacritin signalingachi gatee spasht karel.
karya.
lacritin heey manvi ashru aavaranatil glycoprotein aahe aanee kahee pramanat laal, fuffusanche galane aanee plasmache aahe. heey pramukhyane lacrimal granthidvare tayar hote. kahee lacritin meibomian granthidvare aanee netrashleshmala aanee parpatalachya upakala peshindware dekhil tayar hote. lacritin januk (lackert) heey manvi dolhyatil sarvat pratilekhanit niyantrit janukanpaiki eka aahe. karyatmak abhyas kahee gair-vyutpann upkalachya upakala nutanikaranat bhoomika suchavatat. nalyanmadhun khalchya dishene vahalyane, teey 'pravardhanshil kshetra' nirman karoo shakate. lacritin strav (lipocallin-1 aanee lactopherines), peshi tikoon rahane aanee jakhami jhalyanantar corneal epitheliumche punarutpadan karnyas dekhil protsahan dete. 4 micronometerchya ekagratevar see-terminal lacritin synthetic peptide 'lacrip' (jyala 'aine-94/see-6' asehi mhanatat) saha darroj teen vela samyik upchar, punarutpadit parpatalachya majjatantu aanee undaranchya shushk dolhyanchya pratikrutitil netra prushthbhagavaril upakala. yamule, dolhyancha sarvat samanya aajaar asalelya koradya dolhyachya upcharanmadhye lacritinche chikitsalayin upayog honyachi shakyata vadhate. lasik kinwa pii. aar. keey. shastrakriyenantar barey honyas protsahan denyasathi dekhil heey fayadeshir tharoo shakate. alikadil abhyas asey suchavatat qee lacritin monomer keval koradya dolhyanmadhyech navhe tarr blaferitismadhyehi bhinn ritine niyantrit kele jaate.
lacritin haah eka ell. eff. yuu. abhiyojak mitogen aanee biphasic doses pratisadasah jaganyacha ghatak aahe joou manvi peshinvaril manvi punarsanyojit lacritinsathi 1-10 nmvar ishtatam aahe. under kinwa undaranchya peshinvar kinwa sasachya dolhyanvar manvi lacritinchi uchch sandrata ishtatam asate. alikadil alikadil tappyatil vaidyakeey chachanit, prathamik sjogrens syndromechya rugnanmadhye, jyanche dolhe koradepanache gunank 60 peksha jaast aahet, madhyam teey tivra korade dolhe darshavinare gunank asalelya rugnanmadhye, darroj teen vela lagoo keleli 'lacripip' chee 22 micronumeterchi samyik matra done aathvadyant prabhavi tharali. parpatalitil fluoresineche daag aanee jaljal/dansh heey donhi lakshan qamee jhaale. biphasic matrechya pratisadanusar, 44 micronumeter matra mothya pramanat aprabhavi hotee. biphasic matrechya pratisadat ghanta-aakarachi vakrata asate, jyache doses ishtatam matrepeksha qamee kinwa jaast qamee prabhavi asatat. itar mitogens heey gunadharm samayik karatat. tathapi, makad lacrimal esinar peshinvar makad lacritincha vapar karoon srav tapasanimadhye, matra pratisad 0.12.3 aanee 1 microne meterchya arund matra shrenidware shrenidware lipocallin kinwa lactopherine sravasah sigmoidal asalyache disate. lacrimal granthitoon khalchya dishene lacritin nalikanmadhun dolhyavar vahate.
samanya manvi ashrunmadhun lacritinche krutrim avakshay heey ughad karate qee lacritinchi kamatarata asalelya ashrunmule dahak cytokinesne tanalelya dolhyanchya prushthbhagavaril peshinche astitva tikavoon thevanyas protsahan milat nahee. manvi koradya dolhyanchya ashrunmadhyehi yaa kriyecha abhav asato. tathapi, lacritins purak dolhyanche korade ashru purnapane sanrakshanatmak asatat. tyachpramane, lacritinche krutrimaritya qamee jhalele ashru jivanunashak kriyakalapanmadhye qamee asatat. lacritin qamee karnyasathi vaparali jaanari pratipinde dekhil see-terminal proteophorm qamee karatat. hii nirikshane suchavatat qee serve ashru prathinanmadhye lacritin haah mukhya snrakshak asoo shakato.
dolhyanche korade ashru akali kosalatat, tasech samanya manvi ashru see-terminal proteoformschya krutrimaritya qamee hotat. donhi prakarananmadhye, krutrim lacritin peptides aine-94 kinwa aine-94/see-6 madhye proxy see-terminal proteophorm mhanoon spike karoon sthirata mothya pramanat punarsanchayit kelee jaate. pratyek peptide (oo-esil)-omega-hydroxy fatty aesidmadhye (ohfa) vegane aat shirate, jeey ashru madhye jaleey lipid simevar rahate asey manale jaate. oaafa haah ashru lipidacha ekmev varg aahe joou koradya dolhyat varvar pahata qamee niyantrit keela jaato.
sanket.
lacritin mitogenic, astitva aanee srav sanketancha abhyas keela gela aahe.
lacritin mitogenic signalling done marganche anusaran karateh
pii. keey. see. aii. chya jalad defosphorylationmule teey cytoplasmpasoon golgi upkaran aanee paridhiy kendrakachya kshetrakade kshanikpane jaate. yethe, teey pii. keey. see. aii. aanee pii. ell. see. jee. emm. 2 saha eka sankul tayar karate jyatoon khalchya dishene emm. tii. oo. aar. aanee aine. fatt signalling suru kele jaate. varchya bajus jee. aii. aay. kinwa jee. aii. oo. sanket jee-prathine-jodalelya receptorcha (jee. pii. see. aar.) sahbhag soochit karatat. jee. pii. see. aar. umedvaracha abhyas suru aahe. syndecan-1 bahudha saha-grahankarta mhanoon kaam karate. lacritinla bandhalyane tyachi jee. pii. see. aar. aatmiyata sudharu shakate.
jevha peshinvar taana yeto tevha lacritinche astitva tikoon rahanyache sanket disun yetat. lacritin autofagila tatpurte uttejit karoon jagane aanee homeostasisla protsahan dete. yaa yantranet pratilekhan ghatak foxo 3 chey lacritin uttejit acetylation samavisht asalyache disate. acetylated foxo 3 autophagic madhyasthasathi legand mhanoon karya karate. lacritin autophagic madhyasth aii. tii. jee. 7 saha foxo 1 (jeey tanavasah acetylated hote) chya jodanila dekhil protsahan dete. lacritinchya anupasthitit, konatehi jodani disun yet nahee. tyamule prarambhik davyachya ulat, keval ekte acetylation heey foxo 1-aii. tii. jee. 7 bandhanasathi apure asanyachi shakyata aahe. lecritin peshinna tanavapasun vachavanyasathi oxidative phosphorylation aanee itar chayapachay ghatana dekhil punarsthapit karate.
makadachya lacrimal esinar peshinmadhun ashru prathinanchya lipocallin aanee lactoferrinchya lacritin uttejit sravachi madhyasthi see. aii. 2 + dware kelee jaat asalyache disat nahee, jeey agonist karbacholchya ulat aahe. jevha makadachya lacrimal esinar peshinvar dahak cytokinescha taana yeto (koradya dolhyat ghadato), tevha carbachol lipocalinchya srawala chalana denyachi kshamata gamavato. tathapi, tanav asatanahi lacritin lipocalinchya srawala uttejan dete.
vitaran.
prajati.
samoohadvare ektrit kelelya genomic sequencingmule itar prajatinmadhye anumanit lacritin orthologsche astitva ughad hote. tulanatmak janukeey sanrekhan asey suchavate qee tapasalelya serve gair-adim anukramanmadhye horse lacritin heey manvi lacritinsarkhech aahe. shivay, ghodyachya ashrunmadhye immunoblottingdware kinwa elisadvare heey shodhata yete. manvi lacritinchya see-, parantu aine-, terminskade nirdeshit pratipinde sarvat prabhavi aahet-peshi lakshyikaranasathi aavashyak asalelya see-terminal amphipathic alfa helixchya andajit sanvardhanachya anushangane.
ootee.
manav aanee makadanmadhye uutinchya vitaranachi tapasani karnyat aalee aahe. lacrimal granthimadhye lacritin sarvat jaast vyakt hote, jyat wolferingchya sahayak lacrimal granthicha samavesh aahe. laal granthimadhye abhivyakti madhyam asate aanee stanpayi granthimadhye (karkarog parantu nahee kinwa kvachitach samanya) aanee thyroid granthimadhye kinchit asate. laal granthichi abhivyakti adnyat ductal-sarkhya peshinchya swatantra gatala karanibhoot asalyache disate. fuffusanchya bronchoalvolar lavage aanee plazmamadhye kahee lacritin nondavale gele. lacrimal granthimadhye, dhruvikrut lacrimal esinar peshi sarvat vipul lacritin utpadak asalyache disun yete, jyacha purava carbacol uttejananantar lacritinchya utsarjanashi susangat sravatil danyanchya majboot daganmule midato. carbacol-aadharit utsarjanat pii. keey. see. aanee calcium signalingacha samavesh asato. kahee lacritin meibomian granthidvare aanee netrashleshma aanee parpatalachya upakala peshindware dekhil tayar hote, jyat lacrimal granthisah lacrimal functional unitcha (ell. eff. yuu.) barachsa bhag asato. ektritpane pahilyas, ell. eff. yuu. haah shariratil lacritincha prathamik srot aahe aanee dolha mukhya lakshya aahe.
rog.
korade dolhe haah dolhyancha sarvat samanya rog aahe, joou 5-6% loksankhyela prabhavit karato. rajonivruttinantarchya mahilanmadhye yacha pradurbhav 6-9.8% paryant vadhato aanee vruddhanmadhye 34 takkyanparyant vadhato. ashru jhakan vangan karatat aanee prakashachya apavartanasathi mahatvache asatat. ashru epithelial aarogyala dekhil chalana detat. ashrunmadhil andaje 1543 prathinancha keval eka chhotasa bhag koradya dolhyat bhinn pramanat qamee kinwa niyantrit asato. saumya teey gambhir jaleey kamatarata asalelya koradya dolhyanmadhye aanee contact lensshee sambandhit koradya dolhyanmadhye lacritin monomerche bhinn pramanat niyaman kele jaate. mothya chachanit, jaleey kamatarata asalelya koradya dolhyanchya rugnanche 95 takke ashru lacritin monomerchi kamatarata asalele hote. monomer aanee multimermadhye farak naan naan done abhyasanmadhye koradya dolhyamadhye lacritinemadhye konatahi badal jhalyache aadhalun aale. koradya dolhyanchya undaranchya dolhyanvar kelelya samyik upcharanmule (koradya dolhyache air nockout maus model) fadane purvavat jhaale aanee parpatalachya daag aanee lacrimal granthitil dahak kendrancha aakar donhi dabale gele. blaferitis asalelya rugnanchya ashrunmadhye lacritin monomerchi kamatarata dekhil nondavali gelee. blaferitis hii papanyanchi jaljal aahe jee anekada koradya dolhyashi sambandhit asate. hawaman themb keratopathy" madhye, madhye heey glycosylated nasalele disate. kahee lokanni nondavalelya stanachya karkarogachya samanya sthanikikaranachi pratikruti unigine ('stun granthi' haah stanachya karkarogasathi aahe) aanee janukanchya shreni abhyasanmadhye kelee gelee nahee, parantu kahee stanachya karkaroganmadhye uchch abhivyakti kinwa lackert januk pravardhan disun yete. madhumehachya vividh tappyanvaril madhumehachya ashrunchya itrack vishleshanat madhumehachya drushtipatalvikruti asalelya rugnanchya ashrunmadhye tulanene adhik lacritin, lysozyme, lipophilin aii, lipocallin 1, immunoglobulin lambda cheen aanee lactotransferrin aadhalale. vishleshanane lacritin monomer aanee polymermadhye farak keela nahee aanee biomarker mhanoon sarvancha vapar prastavit keela. burshijanya keratayatismadhil "fugerium solani" dware sansargachya suruvatichya tappyat ashru lacritin monomer kvachitach shodhata yeto. ashru lipocallin-1 aanee cystatine ess heey dekhil qamee niyantrit aahet. africa aanee bharatatil serve parpatalachya vrananpaiki ardhe mhanje burshijanya keratitis heey yaa deshanmadhil andhatvache prathamik srot aahet. prathamik sjogren syndromeshy sambandhit korada dolha asalelya vishayanmadhye 'lacrypeptum' chee dusari tappa chikitsalayin chachani (aine. see. tii. 03226444) purn jhaali aahe. lacrypeptum heey lacritin synthetic peptide 'aine-94/see-6' aahe.

