alkadharmi lysis

alkadharmi lysis kinwa alkadharmi nishkarshan hii aanvik jivashastrat jivanunpasun plasmid dna vegale karnyasathi vaparali jaanari eka paddhat aahe.
paddhat.
rus asalelya plasmid asalelya jivanunna pratham sanwardhan kele jaate, nantar pesheey samagreela (dii. aine. aii. saha) vahak patrachya talashi asalelya golimadhye kendrit karnyasathi namuna centrifuges keela jaato. supernatent fekoon dile jaate aanee nantar golee edda-yukt sharirik buffermadhye punha nilambit kelee jaate. dii. aine. aii. adhogatikarak enzymeschya (dii. aine. aii. ess.) karyasathi aavashyak asalelya emm. jee. 2 + aanee see. aii. 2 + sarkhya dvimiteey dhatuche catayan chillet karane aanee dii. aine. aii. phosphatecha kanaa aanee peshi bhint sthir karnyasathi dekhil kaam karane haah aii. dii. aii. chaa uddesh aahe. peshi futanyapasun rokhanyasathi buffermadhil glucose peshincha parasaran daab rakhel. buffermadhil tri peshi peshicha ph 8 neey rakhoon thewel aanee aar. aine. aii. aar. aine. aii. kadhoon takel jyamule prayogat vyatyay yeil.
swatantrapane, detergent soodiyam dodecil sulfate (ess. dii. ess.) aanee soodiyam hydroxide (naoh) sarkhya majboot ksharasah eka majboot alkadharmi dravan tayar kele jaate aanee nantar misalale jaate. parinami mishran kahee minitansathi ubavale jaate. yaa kalat, detergent peshinchya patalat vyatyay aanate aanee ksharala gunasutra aanee plasmid dna yaa donhicha sampark sadhanyas aanee vikrutee karnyas anumati dete.
ess. dii. ess. neey peshi puttle fadlyanantar, peshinche praman naohla nishprabhavi karel; mhanunach licescha ph 12.8 varoon 12.3 paryant khalee jaato. tyamule jarr puresha jivanu peshi nastil tarr atirikt naoh lahan dii. aine. aii. tukade tayar karnyasathi karya karel. parantu 0.50 mea. ell.-arginine, jeey sthir ph puravatha karoo shakate, 0.01 mea. soodiyam hydroxide badalanyasathi vaparale jaau shakate.
shevati, potassium actate misalale jaate. heey dravan aamlikrut karate aanee dravanatun baher baher gunasutriy dnala navhe, tarr plasmid dnahe punarprakriya karnyas anumati dete. soodiyam dodecil sulfetcha paus padane aanee asha prakare detergent kadhoon takane heey potassiumche aanakhi eka karya aahe. antim centrifugation kele jaate aanee yaveli golimadhye fakt avashesh asatat aanee teey fekale jaau shakatat. plasmidyukt supernatent kaljipurvak kadhale jaate aanee teey aanakhi shuddha kele jaau shakate kinwa jail electrophoresisarkhya vishleshanasathi vaparale jaau shakate.
itar upayog.
tasech, kshariy lisisacha vapar kadhikadhi vanaspatinchi anuvanshik samagri kadhanyasathi keela jaato. vanaspatichya peshinna detergent (samanyatah twin 20 sarkhe zviterionic kinwa noninic detergent) asalele majboot alkadharmi dravan dile jaate aanee mishran uchch tapmanat ubavale jaate. soodiyam hydroxide anuvanshik samagreela haanee pohochavanyachya pravruttimule, dii. aine. aii. tukadyacha aakar qamee jhalyamule hii paddhat varanvar vaparali jaat nahee.

