
fusion jeane

munbu swathanthramaayirunna rund jeenukalil ninnu roopappetta oru hibrid jeenaanu fusion jeane. translocation, interstishyal deletion allengil chromosomal inversion ennivayude falamaayi ithu sambhavikkam. ellah pradhana tharam manushya neoplassiayilum fusion jeenukal kaanappedunnundennu kandethiyittundu. eee fusion jeenukalude thirichariyal oru diagnostic, pravachana marker enna nilayil oru pradhana punk vahikkunnu.
charithram.
aadyathe fusion jeane 1980 kalude thudakkathil cancer koshangalil vivarikkapettu. 1960 ll philadelphiyayile peettar novalum david hungerford chernnu vittumaaraatha myelooid leukemia ulla rogikalil oru cheria asaadhaarana marker chromosome kandethiyathine adisthaanamaakkiyullathaanu eee kandethal-manushyante maarakaavasthayil kandethiya aadyathe sthiramaaya chromosome asaadhaaranatha, pinneedu philadelphia chromosome ennu namakaranam cheythu. 1973ll chikkagoyile jaanett rauli kaanichathu philadelphia chromosome uthbhavichath 9num 22num edayilulla oru parivarthanatthiloodeyaanu, allathe munbu karuthiyirunnathupole 22-aamm chromosome lalithamaayi illaathaakkunnathiloodeyla. 1980 kalude thudakkathil niravadhi anweshakar kaanichathu philadelphia chromosome translocation oru puthiya bcr roopeekaranathilekku nayichuvennu abl 1 fusion jeane, chromosome 9 leeii breakpoyintil "abl 1" jeeninte 3 'bhaagavum chromosome 22 leeii breakpoyintil "bcr" enna jeeninte 5' bhaagavum ulkkollunnu. 1985 ll chromosome 22 leeii fusion jeane vittumaaraatha myelooid raktarbudam undakkaanulla sheshiyulla asaadhaaranamaaya kaimerik bcra: abl 1 protein ulppaadippikkunnuvennu vyakthamaayi.
oongoojenukal.
tumergenesisil anubandha jeane fusion oru pradhana punk vahikkunnuvennu 30 varshamaayi ariyappedunnu. fusion jeenukalkku tumour roopeekaranathinu kaaranamakum, kaaranam fusion jeenukalkku noonn-fusion jeenukalekkaal valare sajeevamaaya asaadhaarana protein utppaadippikkaan kazhiyum. palappozhum, fusion jeenukal cancerinu kaaranamakunna oongojinukalaanu; ivayil bii. sii. aarr-eiii. bii. ell, tell-eiii. emm. ell. 1 (ellaam tii (12; 21)), eiii. emm. ell. 1-eiii. iii. oo (tii (8; 21) ulla emm. 2 eiii. emm. ell), chromosome 21-ll intersteeshyal deletion ulla tii. emm. pii. aarr. ezz. 2-err. jii enniva ulppedunnu, ithu palappozhum prostettu canceril sambhavikkunnu. 
tii. emm. pii. aarr. ezz. 2-aarjinte kaaryatthil, androjan risapttar (aarr) signalling thadassappeduthunnathiloodu oongoojenik itts transcription ghadakam vazhi aarr expression thadayunnathiloodeyum, fusion uthppannam prostettu cancerine niyanthrikkunnu. 
hematological cancerukal, sarcoma, prostettu cancer ennivayil ninnaan mikka fusion jeenukalum kaanappedunnath.
uyarnna nilavaaramulla seres andashaya arbudathinu prathyekavum savisheshavumaaya oru fusion jeenaanu bii. keey. emm-eiii. keey. tii. 2.
rund fusion pankaalikalil ninnu puthiyathum vyathyasthavumaya pravartthanamulla oru jeane uthppannathilekku oongoojenik fusion jeenukal nayichekkaam. pakaramaayi, oru proto-oongoojen sakthamaaya oru promoterumaayi samyojippikkappedunnu, athuvazhi upstream fusion pankaaliyude sakthamaaya promoter moolamundaakunna oru appregulation vazhi oongoojenik function pravartthikkaan sajjeekarikkunnu. randaamathethu lymphomyil saadhaaranamaanu, avide oongoojenukal immunoglobulin jeenukalude promotermaarumaayi chernnupokunnu. trans-spliesing allengil reed-throo evantukal moolavum oongoojenik fusion traanscriptukal undakam.
neoplassiayil chromosomal translocationukal atharamoru pradhana punk vahikkunnathinaal, chromosomal vyathiyaanangaludeyum cancerile jeane fusionukaludeyum oru prathyeka daattaabes srishtichittundu. eee daattaabesine chromosome vyathiyaanangaludeyum cancerile jeane fusionukaludeyum maittalmaan daattaabes ennu vilikkunnu.
roganirnayam.
krithyamaaya roganirnayam nadatthaan cancer diagnosticsil chila chromosomal vyathiyaanangaludeyum avayude falamaayundaakunna fusion jeenukaludeyum saannidhyam saadhaaranayaayi upayogikkunnu. chromosome banding vishakalanam, fluorescence inn sittu hybridisation (fish), reverse transcription polymerase cheyin reaction (arty-pcr) enniva diagnostic laborattarikalil upayogikkunna saadhaarana reethikalaanu. cancer genomukalude valare sankeernamaaya swabhaavam kaaranam eee reethikalkkellam avayude prathyeka poraaymakalundu. uyarnna-throoput sequencing, ishtaanusritha dna microaarekal thudangiya sameepakaala sambhavavikasangal kooduthal kaaryakshamamaya reethikal avatharippikkumenn vaagdaanam cheiyunnu.
parinamam.
jeane vaasthuvidyayude parinaamatthil jeane fusion oru pradhana punk vahikkunnu. coding sequenceukalil jeane fusion sambhavikkukayaanenkil namukk athinte prabhavam nireekshikkaan kazhiyum. erattathaappu, sequence divergence, recombination ennivayaanu jeane parinaamatthil pravartthikkunna pradhana sambhavanakal. eee sambhavangalkk ithinakam nilavilulla bhaagangalil ninnu puthiya jeenukal utppaadippikkaan kazhiyum. noonn-coding sequence mekhalayil jeane fusion sambhavikkumbol, athu mattoru jeeninte sis-regulatory seekvansinte niyanthranatthilulla oru jeeninte aavishkaarathinte thettaaya niyanthranathilekku nayichekkaam. ithu coding sequenceukalil sambhavikkukayaanenkil, jeane fusion oru puthiya jeeninte assemblic kaaranamaakunnu, appol ithu oru multy-domaine proteinil pepttaid moduleukal cherkkunnathiloode puthiya pravartthanangal kaanaan anuvadikkunnu. valia jaiva thalatthil jeane fusion sambhavangal kandethunnathinulla reethikalkku proteinukalude multy modular aarkkitekcharinekkurich ulkkaazhcha nalkaan kazhiyum.
purine biosynthesis.
saarvathrika janithaka kodile naalu vivara encoding aditharakalil randennam purains adenine, gwanine ennivayaanu. eee pyoorinukalude jaivasamshleshanam sambhavikkunnath archaea, bacteria, yookkariyottukal ennee jeevajaalangalude moonnu mekhalakalile vyathyastha speesheesukalile samaanavum ennaal samaanamallathathumaaya paathakaliloodeyaanu. rando athiladhikamo purine biosynthetic ensimeukal orotta jeane encode cheiyunna jeane fusionukalude vyaapanamaanu baakteeriyayile purine biosynthetic paathakalude oru pradhana savisheshatha. biosynthetic pathayil thudarchayaaya gattangal nadathunna ensaimukale encode cheiyunna jeenukalkkidayilaanu atharam jeane fusionukal mikkavarum prathyekamaayi sambhavikkunnath. eucariotic speesheesukal saadhaaranayaayi bakteeriyakalil kaanapedunna ettavum saadhaaranamaaya jeane fusionukal pradarshippikkunnu, ennaal koodaathe upapachayam vardhippikkaan saadhyathayulla puthiya fusionukalum undu.
kandethal.
sameepa varshangalil, adutha thalamura sequencing saankethikavidya ithinakam thanne ariyappedunnathum puthiyathumaaya jeane fusion sambhavangal genome wide skailil screen cheyyaan labhyamaayittund. ennirunnaalum, valia thothilulla kandethalinulla munvyavastha koshatthinte transcriptominte oru jody-end sequencingaanu. fusion jeane kandethalinte disha pradhaanamaayum daatta vishakalanathilekkum drishyavalkkaranathilekkuma. traanskript thalatthil kandethiya jeane fusionukal nerittu drishyavalkkarikkunnathinna chila gaveshakar ithinakam traanscriptom viewer (twear) enna puthiya upakaranam vikasippicheduthittundu.
gaveshana aaplikkeshanukal.
jeevashaasthranjar gaveshana aavasyangalkkaayi manappoorvvam fusion jeenukal srishtikkukayum cheythekkaam. thaalpparyamulla jeenukalude niyanthrana gadakangalumaayi reporter jeenukalude samyojanam jeane expression padikkaan gaveshanangale anuvadikkunnu. jeane regulettarmaarude pravartthana nilavaaram alakkaanum jeenukalude niyanthrana sittukal thirichariyaanum (aavashyamaaya signalukal ulppede) ore uthejanathodulla prathikaranamaayi niyanthrikkappedunna vividha jeenukal thirichariyaanum prathyeka koshangalil aavasyamulla jeenukalude aavishkaaram krithrimamaayi niyantrikkaanum reporter jeane fusionukal upayogikkaam. udaaharanathinu, thaalpparyamulla oru proteinteyum pacha fluorescent proteinteyum fusion jeane srishtikkunnathiloode, fluorescence microscopy upayogichu koshangalilo tissuevillo thaalpparyamulla protein nireekshikkaam. oru fusion jeane prakadippikkumbol samanvayippikkunna proteine "fusion protein" ennu vilikkunnu.

