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manushyaril "gyaard" jeane encode cheiyunna oru proteinaan gamma-aminobutic assid risapttar subunit deltta. sasthanikalude thalachoril, deltta (δ) subunit qoo-assembly vazhi nirddishta gaba risapttar subtypukal roopappeduthunnu, ithu gaba resepterukal (δ-gaba resepterukal) adangiya δ subunitilekku nayikkunnu.
ghadanayum pravartthanavum.
hettero-pentameric δ-gaba resepterukalude upavibhagangalilonnaaya deltta (δ) subunit, gaba modulate cheiyunna δ-gaba resepterukalude nirddishta sellular localisationulla oru nirnnayaka sabyoonittaan. sasthanikalude thalachorile pradhana inhibittari neuro traansmittaanu gaba, avide ithu ligand gated chloride chaanalukalaaya gaba resepterukalude shekharathil pravartthikkunnu. 19 sabyoonittukalude (α1-α6, β1-β3, γ1-γ3, δ, ω, θ, π, ρ1-ρ3) kudumbatthil ninnulla asamblikal ulppede vaividhyamaarnna subunit poolil ninnaan ithu kootticherkkunnathu. gyaard jeane deltta (δ) sabyoonittine encode cheiyunnu. prathyekichum, δ-subunit saadhaaranayaayi extraasainaaptik pravartthanavumaayi bandhapetta gaba resepterukalil prakadippikkunnu, ithu madhyavarthithva tonic inhibition aanu, ithu classical inhibishane (phaasik allengil synaptic inhibition) apekshichu mandagathiyilaanu. ettavum saadhaaranamaaya gaba resepterukalkku gamma subunit undu, ithu resepterine benzodiasepines bandhippikkaan anuvadikkunnu. ekkaaranathaal, δ-subunitukal (δ-gaba risapttar) adangiya resepterukale chilappol "benzodiasepine-insensitteevu" gaba resepterukal ennu vilikkunnu. ennirunnaalum, ethanolinode prathikarikkaattha benzodiasepine sensitiivu resepterukalumaayi thaarathamyappeduthumbol avaa ethanolinode athisayakaramaaya uyarnna samvedanakshamatha kaanikkunnu, ennirunnaalum eee phalangal saahithyathil poornnamaayum sthireekarichittilla. resepterukal adangiya δ-subunit thalachorinte hippocampasile ventral tegmental eriya (vii. tii. eiii) pathayil ulppettirikkunnathaayi ariyappedunnu, athaayath avaa padanatthilum oormmayilum prathiphalatthilum swaadheenam chelutthiyekkaam.
gaba resepterinte cloning.
shudheekaricha (bowine braine) resepterukalil ninnu labhikkuna pepttaid sequenceukal braine cdna librarikal parishodhikkunnathinaayi synthetic dna probukal nirmmikkaan upayogichirunna classical reethiyilaan gaba resepterukal aadyam kloon cheythath. oduvil, eee saankethikavidya jeane kudumbatthinte bhooribhaagatheyum athinte isophomukal upayogichu thirichariyaan kaaranamaayi α1-α6, β1-β3, γ1-γ3 subunitukal, oru δ subunit.
kosha tharam-nirddishta aavishkaaram.
13 gaba risapttar sabyoonittukalude emrnakalude sellular localisation vividha mastishka pradeshangalil vishakalanam cheythu. udaaharanathinu, seribellathil, cerebellar granuel koshangalilum parkinje koshangalilum vividha risapttar upavibhagangal kaanapedunnu, athesamayam ghraana bulb, periglomerular koshangal, tuft cheytha koshangal, aantharika granuel koshangal ennivayil gaba risapttar upavibhagangal prakadippikkunnu. prathyekamaayi, sell tharam-nirddishta δ subunit eksprashante paatten chuvadeyulla pattikayil kaanichirikkunnu.
kvaanditteevu reverse traanscriptes pcr digital pcr thammilulla saankethika thaarathamyathil, elii "gabbard" jeeninte aavishkaaram somattosensari courtexile moonnu kosha tharangalil parishodhichu neurogliaphom cellukal, phaast spiking basket cellukal, pyramid cellukal. moonnu kosha tharangalilum jeane expression kandethiyenkilum neurogliaphom koshangalil mattu kosha tharangale apekshichu ganyamaaya sambushteekaranam kaanichu. ethanolumaayi vittumaaraatha edavittulla sanbarkkam moolam gaba risapttar deltta subunit aazhathil niyanthrikkappedunnu, ithu paatholajikkal alcohol aashrithathvathinu shakthamayi sambhavana cheiyunnuvennu thonnunnu.
δ-subunit gfp tagging.
kadathal, praadeshikavalkkaranam, oligomerisation, prasakthamaaya risapttar upavibhaagangaludeyum prasakthamaaya upavibhaagangaludeyum protein edapedalukal enniva padikkunnathinaayi gaabayude sub unitukalkku green fluorescent protein (jii. eph. pii) allengil athinte vakabhedangal (iii. jii. eph. pii) tagg cheythu. saadhaaranayaayi, prasakthamaaya sabyoonittinte pakwathayulla pepttaid shreniyude enn-terminus allengil sii-terminasilaanu iii. jii. eph. pii allengil jii. eph. pii tagging nadathunnathu. enn-terminus, sii-terminus, intra sellular (cytoplasmic) domaine thudangiya sabyoonittinte vividha domainukalilaanu δ-sabyoonittinte jii. eph. pii tagging nadathiyathu. ennirunnaalum, ivayum mattu padanangalum undaayirunnittum, saahityam parasparavirudhamaaya phalangal soochippikkunnathinaal eee upavibhaagathin membran taargettinginaayi α, β upavibhagangal aavashyamaano ennu vyakthamalla. eee sabyoonittinte jii. eph. pii tagging upayogichukondu, δ sabyoonittinte sell membran expression α, β sabyoonittukalude saannidhyathil maathrame nireekshikkappettitlu ennu oru group report cheythu. ennirunnaalum, δ sabyoonittin kosha stharathe lakshyamidaamennum βδ adangiya resepterukal nilavilundennum mattoru samgham nirddeshichu.

