
trimethylamine enn-ooksaid reductase

electron transport cheyinte bhaagamaayi trimethylamine "enn"-ooksaid reductase (toorr allengil tamo reductase, iii. sii. <aii. dii. 1>) ennathu trimethylamine "enn"-ooksaid (timo) trimethylamine (tii. emm. eiii) aayi kuraykkaan kazhiyunna oru microbial ensaimaanu. iii" yill ninnaan ensime shudheekarichathu. koliyum photosynthetic baakteeriyaya rosobactor denitrifance.
malsyathinte koshangalil uyarnna saandrathayil trimethylamine ooksaid kaanapedunnu, eee samyuktham durgandhamulla trimethilaamainaayi bakteeriyakal kuraykkunnathu malsyam kedaakunnathil oru pradhana prakriyayaan.
vargeekaranam.
tii. emm. eiii. oo. reductasinu oru ensime kammeeshan (iii. sii) nambar <aii. dii. 1> undu. iii. sii nambarukal ensime naamakaranatthinte oru samvidaanamaanu, eee naamakaranatthinte oro bhaagavum athinte prathikaranavumayi bandhappett ensaiminte purogamana vargeekaranathe soochippikkunnu. aadya nambar prathikarana tharam nirvachikkunnu, randaamathe nambar ulppetta samyukthangalekkurichula vivarangal nalkunnu, moonnaamathe nambar prathikaranatthinte tharam vyakthamaakkunnu, naalaamathe nambar oro ensimine savisheshamaaya seeriyal nambar poortthiyaakkunnu.
trimethylamine enn-ooksaid reductasinu isi nambar 1.7.2.3 undu, eee gadakangal inipparayunna ensime vargeekaranangale soochippikkunnu
speeshees vitharanam.
yooriyayude uyarnna saandrata polulla sammarddangalil ninnu proteinukale samrakshikkunnathil upayogapradamaaya jaiva pravartthanamulla oru jaiva ausmoliteanu tmavo. glycerol polulla pulippikkaanaavaatha kaarban srothassukalil valaraan anuvadikkunna oru badal electron transport cheyin aayi tmavo upayogichu vividha bakteeriyakal vaayurahithamayi valarunnu. moonnu vyathyastha paaristhithika edangalil tii. emm. eiii. aayi kuraykkaan kazhivulla bakteeriyakal kaanapedunnu. samudrathile bakteeriyakalilum aazhamillaatha kulangalil vasikkunna photosynthetic bakteeriyakalilum enterobacteriyakalilum tmavo-reducing innuvare nireekshikkappettundu.
niravadhi jeevikalil tmavo reductaseukal padikkappettittund, ariyappedunna ellah terminal ensaimukalilum oru molybdinum coffactorinte saannidyamaanu oru saadhaarana samrakshitha savisheshatha.
substrate prathyekathayude adisthaanathil eee ensaimukale rund grouppukalaayi tirikkaam
aadya groupil "escherichiya koolii", "shevanella putrephasians", "rosobactor denitrifance" thudangiya enangalum randaamathe groupil "proteus vulgaris", "rhodobacter capsulatus", "rhodobacter spheroids" thudangiya enangalum ulppedunnu.
tmavo shvasanavyavasthaye kooduthalum "iii" ennathil tanmatra thalatthil vyaapakamaayi padichittundu. koolii, rhodobacter enangal.
prathikarana samvidaanam.
iii. yill. koolii, "tmavo reductase" ennathu "toorr" caid oopparan encode cheiyunnu. tork jeane oru pentahemic sii-type cytochrome (tork) encode cheiyunnu. tora jeane encode cheytha periplasmic tora terminal ensaimilekku tork electronukale nerittu kaimaran saadhyathayund. torcad oopparaninte vaayurahithamaya aavishkaaram tmoo allengil anubandha samyukthangalude saannidhyathaal karsanamaayi niyanthrikkappedunnu.
tmavo tmay ulppedunna niravadhi vyathyastha upapachayam vazhikalundu. electron transport cheyinte bhaagamaayi tmavo reductase uthejippicha tmavo tmailek kuraykkunnathu inipparayunna prathikaranatthe pinthudarunnu
naad + echu + + trimethylamine enn-ooksaid formula _ 1 naad + + trimethylamine + echu2oo
ennirunnaalum, randum aarr. denitrifance ", iii. koolii ensaimukalkku cytochromukalil ninnu electronukal sweekarikkaan kazhiyum
tmavo tmay ulppedunna mattu prathikaranangalil iva ulppedunnu
ghadana.
iii. yill. koolii, "inducble, periplasmic tamo reductase mikkavarum ellah timo kurravinum kaaranamaakumenn theliyikkappettittund (bakkiyullava dmso kuravaanu). athesamayam ithinte gadanaaparamaaya vishakalanam illa. shevanella masilia" yill ninnulla tmavo reductase 2.5 Â resolutionil verthirichedukkukayum savisheshatha kaanikkukayum cheythathayi koolii "ensime report cheythittundu.
niravadhi jeevikalil tmavo reductaseukal padikkappettittund, ariyappedunna ellah terminal ensaimukalilum oru molybdinum coffactorinte saannidyamaanu oru saadhaarana savisheshatha. molybdopterine thanmaathrayude pothuvaaya roopam oru pairan valayavumaayi samyojippicha oru terrin group ulkkollunna oru tricyclic ring sistamaanu. eee pairan valayathinte punk molybdinum coffactorinte ooksideshan avastha niyanthrikkunnathino prottoon vyaapanam sugamamaakkunnathino ulla oru margamaakaam. koodaathe, sajeeva kendrathilekku nayikkunna kuzhal polulla praveshana kavaadatthile sugandha avashishtangalude krameekaranam dmso reductase gadanakalumaayi adutha bandhamullathaan. rund tryptophaanukalum rund tyrosine avashishtangalum chernnu roopappedunna oru hydrophobic pokkattum tmavo reductasil adangiyirikkunnu, koodaathe uyarnna parirakshitha avashishtangalum adangiyirikkunnu.
tmavo reductase oof "s thaarathamyam cheyyumbol. massilia "muthal dii. emm. ezz. oo reductase vare" aarr. spheroids "," aarr. capsulates, "mothatthilulla ghadana shradheyamaayi samaanamaan. ennirunnaalum, tmoo reductasile oru pradhana vyathyasam "aarinte dmso reductasil kaanaatha tyrosine (tyre114) aanu. capsulatus ". ithu tmavo reductasil oru thrionin (thr116) upayogichu maattisthaapikkappedunnu, eee avashishtathinu chuttumulla nattellu, avashishtam 100 muthal 116 vare, dii. emm. ezz. oo reductasukalilethinu samaanamalla. molybdinum active sentarinu sameepamulla vishalamaaya axus cheyyaavunna sthalamaanu avasheshikkunnathinte nerittulla anantharafalam, ithu dimethylsulfoxide thanmaathrakalekkaal kurachu bulkier trimethylamine-ooksaid thanmaathrakale eluppathil ulkkollaan saadhyathayund. oru ensaiminte roopam ellaayppozhum athinte pravartthanavumaayi nerittu engane bandhappettirikkunnave eee vyathyasam kaanikkunnu.
ennirunnaalum, tmavo reductase active saittinte gadanayekkurichu sameepakaala vyathyaasangal uyarnnu. nirddishta sajeeva saittil niravadhi aswaabhaavika boond dairkhyangal adangiyirikkunnu; oru moow-ooo bondinte neelam moow-ooo single-boond ekopanathinu valare cheruthaanu, koodaathe naalu moow-ezz bondinte neelam pratheekshichathilum valare kooduthalaanu. koodaathe, sajeevamaaya saittinte nirddishta molybdinum ekopanam valare thirakkeriyathaan, niravadhi nonbonding attangal thammilulla dooram avayude vaan derr walls rediyude aakethukayekkaal ganyamaayi kuravaanu, chila boond konukal yukthirahithamayi cheruthaanu. ippol, eee tirakk ensaiminte onniladhikam roopangalude cochrystalisation moolamaanennu anumaanikkappedunnu.

