
rde-1

synorhabiditis elegansil" aarr. enn. eiii-mediated interferencinu (aarr. enn. eiii) aavashyamaaya oru praadhamika aargonaut proteinaan aarr. dii. iii-1 ("aarr" naayi-"dii" fective "1"). rde-1 jeane locus aadyamaayi "sii" yill savisheshatha nalki. eleegaansinte mutentukal rainiye prathirodhikkunnu, koodaathe plaant, drosophila, kasherukkal enniva ulppedunna valare samrakshikkappetta pivee jeane kudumbatthile amgamaanu.
ranveer paathayile punk.
praadhamika sirna-argonuit samuchayam.
koshatthilekku ulkkollumbol, exogenous trigger dsrna rde-4 kondu bandhippikkukayum rde-1 ulppedunna oru dieser complexes vazhi 21-25 enty primery sirnayayi pilarukayum cheiyunnu. praadhamika sirna rde-1 lake bandhippikkukayum thudarnnu rna-induced silencing complexinte (risk) roopeekaranathe prolsaahippikkukayum cheiyunnu. drosophilayilum manushyarilum ulla mattu aargonoottukalil ninnu vyathyasthamayi, rde-1 leeii kaattalitik arnez echu motif taargett traanscriptinte slicer pravartthanam pradarshippikkunnathaayi kaanichittilla. pakaram, rde-1 leeii arnez echu pravartthanam praadhamikamaayi yaathrakkaarude izhayude pilarppiloode sirna pakwata sugamamakkunnu.
secondary sirna-argonuit complexes.
praadhamika aargonaut complexes secondary sirnakal srishtikkaan oru rna-dependent rna polymerase (rdrp) recruit cheiyunnu, ithu oru amplification prathikaranatthinu kaaranamaakunnu. dvitheeya sirnakal degenerate dvitheeya aargonauttukalaal bandhippichirikkunnu, avaa taargett traanskript degradationil nerittu ulppedunnu. ennirunnaalum, kooduthal samriddhamaaya dvitheeya sirnakalumaayi rde-1 sthiramaaya bandhamonnum kanikkunnilla.
trigger dsrnaude uyarnna saandrathayiloode rde-4 kuravu veendedukkaan kazhiyumenkilum, secondary aargonaut pravarthanaparamaaya redendency prakadippikkunnu, rde-1 kurravulla myoottantukalil rna-mediated silencing punasthaapikkaan kazhiyumennathinu thelivukalonnumilla. eee paridhivare, rde-1 nuu exogenous raie pathayil gunaparamaayi vyathyasthamaaya pravarthanamundennu thonnunnu.
ghadana.
canonical aargonaut proteinukalkku oru chandrakkala aakruthiyilulla adithara roopappeduthunna moonnu praadhamika domainukal undu paas, midu, pivi domainukal. paas, midu ooriyant enniva yathaakramam 3 ', 5' terminikalumaayi bandhippichu eratta-stranded sirna nankooramidukayum aantharika nucleotideukal bays jodiyakkaan praapyamaakkukayum cheiyunnu. pivis domaine oru arnez echu-polulla gadanayilekku madakkappedunnu, koodaathe samrakshitha kaattalitik triad "dii. dii. echu" (rund aspaarttett avashishtangal, oru histidin avashishtam) adangiyirikkunnu. rde-1-nte kristtal ghadana aupachaarikamaayi vyakthamaakkiyittillengil athinte manushya homologukalumaayi saamyamundennu anumanikkaam.
yaathrakkaarude izhayude apachayam.
rnase h kaattalitik triadile eathenkilum avashishtangalude mutation manushya aargonaut protein bind 2 leeii slicer pravartthanam nirtthalaakkunnu, ithu rnase h domaine taargett mrna degradation nerittu utharavaadiyaanennu soochippikkunnu. ennirunnaalum, rde-1 marnah-kleevej pravartthanatthil ulppettitilla.
pakaram, samrakshitha dii. dii. echu. rooparekhayil mutationukalulla rde-1 kuranja passenture (sense) strand vittuvarav prakadippikkunnu, ithu arnez echu pravartthanam passenture strandine pilarthaan sahaayikkunnuvenn soochippikkunnu, ithu guide (antisense) strand bays-pairinginu emrna lakshyamidunnathinu axus cheyyaan anuvadikkunnu. koodaathe, single-stranded sirna loducheyyunnathiloode dii. dii. echu motif myoottantukalil taargett silencing poornnamaayum punasthaapikkaan kazhiyum, ithu raie paathayile oru thaazhekkulla gadakamaanu slicer pravarthanatthinu utharavaadiyennu soochippikkunnu. athinaal, rde-1-nte arnez echu domaine sirna pakwata sugamamakkunnu, pakshe taargett mrna traanscriptukal pilarthunnathil nerittu ulppedunnilla.