
locus niyanthrana mekhala

vidoora kromaattin saittukalil linkucheyta jeenukalude aavishkaaram vardhippikkunna oru deerghadoora sis-regulatory gadakamaanu locus kantrol reejian (lcr). erythroid koshangalile β-globin jeenukalude selective expressionil kaanunnathupole ithu koppi nambarine aasrayichulla reethiyil pravartthikkunnu, ithu tissue-nirddishtamaanu. promotermaar, enhancerukal, sailensarukal thudangiya lcr, jeane-proximal gadakangal upayogichu jeenukalude expression levelukal parishkkarikkam. kromaattin-modify cheyyal, qoo aaktivettar, transcription complexukal ennivaye recruit cheythaanu lcr pravartthikkunnathu. athinte kramam pala kasherukkalilum samrakshikkappedunnu, koodaathe nirddishta sthalangalude samrakshanam pravartthanatthil praadhaanyam soochippichekkaam. transcription complexinte recruitmentiloode deerghadoora "sis" niyanthranam nadathunnathinaal ithu oru suuppar-enhaansarumaayi thaarathamyam cheyyappedunnu.
charithram.
transgenic elikalekkurichulla padanangalil 20 varsham munbu β-globin lcr thiricharinjirunnu. beetaa-globin jeane eksprashante saadhaarana niyanthranathinu lcr aavasyamaanennu eee padanangal nirnnayichu. eee adhika niyanthrana gadakathinte saannidhyathinte thelivukal β-globin klasttarinte mukalilaayi 20 kebi mekhala illaatha oru koottam rogikalil ninnaan vannath, athu eathenkilum β-globin jeenukalude prakadanatthinu pradhanamaayirunnu. ellah jeenukalum mattu niyanthrana gadakangalum kedukoodaathe undaayirunnuvenkilum, eee domaine illaathe, β-globin klasttarile jeenukalonnum prakadippikkappettil.
udaaharanagal.
peru soochippikkunnathu lcr orotta pradeshathu parimithappeduthiyirikkunnum, eee soochana β-globin elcrinu (echbibi-lcr) maathrame baadhakamaakoo. oru lcr athu niyanthrikkunna jeenukalkku chuttum akathum onniladhikam pradeshangalil vitharanam cheyyaan kazhiyumennu mattu padanangal kandethiyittundu. elikalilum manushyarilum β-globin lcr aadyathe globin jeeninte (epsiloon) mukalilaayi 6-22 kb aayi kaanapedunnu. ithu thaazhepparayunna jeenukale niyanthrikkunnu
eee jeenukalude mukalilulla manushyante ex chromosomile opn1eldablyuvinte aavishkaaravum opn1eldablyuvinte aadya pakarppukalum niyanthrikkunna oru opsinlcr (opsin-lcr) undu. pravarthanarahithamaaya lcr rund oopsinukaludeyum aavishkaaram nashtappedutthukayum neela koonn monochromacyilekku nayikkukayum cheyyum. seabrafish ulppedeyulla teleost malsyangalilum eee lcr samrakshikkappedunnu.
2002 leeii kanakkanusarichu manushyaril 21 laksham kodi pradeshangal ariyappedunnu. 2019 leeii kanakkanusarichu 11 manushya ell. sii. aarr. kall enn. sii. bii. aii daattaabesil rekhappeduthiyittundu.
lcr functionte nirddishta modalukal.
lcr engane pravartthikkunnu ennathinte oru maathruka thirichariyaan padanangal nadathiyittundengilum, inipparayunna modalukalude thelivukal shakthamayi pinthunaykkukayo ozhivaakkukayo cheiyunnilla.
looping model.
transcription gadakangal hypersensitive saitt korukalumaayi bandhippikkukayum lcr athu niyanthrikkunna jeeninte promoterumaayi samvadikkaan kazhiyunna oru lupe roopappeduthukayum cheiyunnu.
tracking model.
transcription gadakangal elcrumaayi bandhippichu oru samuchayam roopappeduthunnu. athu niyanthrikkunna jeeninte promoterumaayi bandhippikkaan kazhiyunnathuvare complexes dna helixiloode neengunnu. orikkal bandhippikkumbol, transcriptional upakaranam jeane expression vardhippikkunnu.
sugamamaya tracking model.
eee sidhaantham looping, tracking modalukal samyojippikkunnu, transcription gadakangal elcrumaayi bandhippichu oru lupe roopappeduthunnu, athu athu niyanthrikkunna jeeninte promotere thedukayum bandhippikkukayum cheiyunnu.
link model.
noonn-dna-binding proteinukalum kromaattin modifierukalum upayogichu transcription gadakangal kramamaya reethiyil elsiaaril ninnu promoterumaayi dnaumayi bandhippikkunnu. ithu transcriptional domaine thurannukattunnathinaayi kromaattin roopeekaranathe maattunnu.
elcrumaayi bandhapetta rogangal.
transgenic elikalil nadatthiya padanangal kaanikkunnath β-globin lcr illaathaakkunnathu chromosominte pradesham hetarokrometik avasthayilekku ghaneebhavikkaan kaaranamaakunnu ennaanu. ithu β-globin jeenukalude aavishkaaram kurayunnathilekku nayikkunnu, ithu manushyarilum elikalilum β-thalaseemiyaykk kaaranamakum.

