
mipol1

sii. sii. dii. sii193 (193 adangiya coiled-koyil domaine) ennum ariyappedunna mipol 1 (mirror imej polydactily 1) manushyaril "mipol 1" jeane encode cheiyunna oru proteinaan. eee jeeninte parivartthanam mirror-imej polydactyliyumaayi (lorin-sandro syndrome ennum ariyappedunnu) bandhappettirikkunnu.) manushyaril, ithu akkangalude mirror-imej duplication savisheshathayulla oru apoorva janithaka avasthayaanu.
jeane.
sii. sii. dii. sii. 193 (193 adangiya coiled-koyil domaine) ennum mipol 1 ariyappedunnu.
locus.
15 exonukalum 11 intronukalum ulkkollunna mypol 1 jeane plus strandle 14q13.3-q21.1 ll sthithicheyyunnu, bays jodikal 37,197,888 muthal 37,579,207 vare (manushya grachu 38 primery asambliyil, neelam 381,320 bays jodikal) vyaapichukidakkunnu. athinte ayalpakkathulla chila sradheyamaaya jeenukalil slc25eiii21 (eee jeeninte mutation synpolydactylic kaaranamaakunnu), foxa 1 enniva ulppedunnu.
marnah.
badal spliesing vazhi uthppaadippikkunna kuranjathu 15 splies isophomukalenkilum mipol 1-ll undu.
protein.
swathukkal.
manushyaril parishkkarikkappedaathu mipol 1 protein isophome 1 nuu 5.6 aiso electric pooyintum 51.5 kiloda tanmatra bhaaravumund. mattu manushya proteinukalumaayi thaarathamyappeduthumbol, asaadhaaranamaamvidham kuranja alavil prolin, glycine, uyarnna alavil glutamic assid, glutamine enniva adangiyirikkunnu.
isophomukal.
manushyaril eee proteininte kuranjathu moonnu isophomukalenkilum badal spliesing vazhi uthppaadippikkappedunnu isophome 1, neelam 442 amino aasidukal, isophome 2 neelam 261 amino aasidukal, isophome 3 neelam 169 amino aasidukal.
domainukalum rooparekhakalum.
mipol 1-ll 107-212,253-435 sthaanangalil (<aii. dii. 1> ll kaanichirikkunnu) athinte sii-terminasil rund coiled-koyil domainukal adangiyirikkunnu. 128-143 sthaanathu oru ubhaykakshi nuclear localisation signal pravachikkappedunnu. 
vivarthanaananthara mattangal.
mipol 1-nulla bioinformatics upakaranangal upayogichu inipparayunna vivarthanaananthara parishkkaranangal pravachikkappedunnu. eee proteininaayi onniladhikam phosphorylation sittukal pravachikkappedunnu, avaa oru cassin kainas 1 (sike 1) saitt, moonnu cassin kainas 2 (sike 2) sittukal, moonnu neck 2 sittukal ennivayulppede aduthulla oorthologukalil samrakshikkappedunnu.
ghadana.
mipol 1-nte krithyamaaya ghadana ithuvare vyakthamakkiyittilla. athinte moonnaam gadanayekkurichulla homology adisthaanamaakkiyullathum puthiya pravachanangalum soochippikkunnathu athil parasparam bandhippicha alfa helikkukal adangiyirikkaam, ithu coiled-koyil domainukal roopappeduthunnu (fig.4 kaanuka.).
sub sellular localisation.
manushyante yuu2oesu kosharekhayile (asthi austiosaarkkoma epithelial koshangal) immunofluorescence imaging cytosolile praadeshikavalkkaranam kaanikkunnu. manushya prostettu tissuevinte immunohistokemisro imaging cytosolic localisation soochippikkunnu. 128-143 sthaanathu oru ubhaykakshi nuclear localisation signal pravachikkappedunnu, ithu sasthanikalude oorthologukalil valareyadhikam samrakshikkappedunnu (fig.2 kaanuka.), ithu nucleusil saadhyamaaya localisatione soochippikkunnu.
jeane niyanthranam.
eee jeeninte pravachicha promoter sequence bays jody 37196852 muthal 37198126 (1,275 bipi) vare vyaapichukidakkunnu, koodaathe gaatta binding gadakangal, smad 3, tipi 63, enrf 1 thudangiya transcription gadakangalkkaayi onniladhikam pravachicha binding saittukalund.
jeane expression.
manushyaril kuranja alavil, prostettil ettavum uyarnna prakadanatthode, mipol 1 sarvavyaapiyaayi prakadippikkunnu.
traanskript niyanthranam.
pravachicha onniladhikam stem looppukal (bioinformatics upakaranangal upayogichu) rna dvitheeya ghadanaye sthirappeduthukayum adutha bandhamullah speesheesukaliludaneelam samrakshikkukayum cheiyunnu. mirr 3163, mirr190eiii thudangiya micrornakalkku onniladhikam binding targettukal kandethiyittundu, avaykku eee pradeshangale marnayil nishabdamaakkaanum vivarthanathe thadayaanum kazhiyum.
klinikkal praadhaanyam.
mipol 1 jeane oru autosomal dominant jeenaanu. manushyaril syndromic allatha polydactylic kaaranamakunna aarr jeenukalil onnaanith (i.iii. mattu anubandha vairudhyangalonnumille oru prathyeka sambhavamaayi polydactily sambhavikkunnu). eee jeeninte parivartthanam manushyaril mirror-imej polydactyliyumaayi (lorin-sandro syndrome ennum ariyappedunnu) bandhappettirikkunnu, ithu kaikalileyum kaalukalileyum akkangalude mirror-imej duplication savisheshathayulla oru apoorva janithaka avasthayaanu.
eee jeane kendra naadeevyavasthayude vikasanavumayi bandhappettirikkunnu, eee jeeninte nashtam craniophasial vaikalyangalkkum corpus kalosathinte ajenisisinum kaaranamakum.
pii21 (waf1/sip1), pii27 (saiklin-ashrita kainaasukal aaya proteinukal, sell saikkil arast vazhi tumour adichamarthunnathumaayi bandhappettirikkunnu) ennivayude upgradulationiloode nasopharinchal carsinomayil (enpc) jeane oru tumour saprassaraayi pravarthikkunnathaayi kaanikkunnu. cancer purogathiyil mipol 1 jeeninte punk anweshikkunna mattoru patanam report cheythath enpc tumour tissuekalil mipol 1 niyanthrikkappettirum krithrimamaayi jeenine veendum prakadippikkunnathu angiogenic gadakangale niyanthrikkunnathiloode metaastaasisumaayi bandhapetta proteinukalaaya ekedi, pii 65, fac 14 ennivayude phosphorilation kuraykkunnathiloodeyum tumour adichamarthalinu kaaranamayennum. mipol 1 ariyappedunna tumour adichamarthunna mattoru jeenaaya robumaayi edapazhakunnu, eee edapedal roob pravartthanam vardhippikkunnathinu sthireekarichu. 
pediatric hye grade glioma (phgc) kurichulla oru padanatthil, uyarnna rakthakkuzhalukalile tumourukalil mipol 1 jeane daun-regulated 2.4-fold aanennu kandethi.
vairal aarenekalude transcription replication ulppedunna proteinaaya saars-cove 2 leeii replicase polyprotein 1ebumaayi protein prathipravarthikkunnathi ariyappedunnu.
parasparaprathikarana proteinukal.
eee protein onniladhikam manushya proteinukalumaayi prathipravarthikkunnathi ariyappedunnu, ithu rund-hibrid screening vazhi parishodhikkappedunnu. chila sradheyamaaya udaaharanagal ivayaanu
laatts 2: koshangalude vyaapanam niyanthrikkunnathiloode apoptosis prothsaahippikkunnathilum avayavangalude valippam niyanthrikkunnathilum tumour adichamarthunnathilum pradhana punk vahikkunna hippo signalling paathayile yaap 1 neey negatteevu aayi niyanthrikkunnu.
zgpat (jii paachu domaine adangiya proteenodukoodiya sink finger cch-type): koshangalude vyaapanam, athijeevanam, migration ennivayil ulppedunna oru jeenaaya egfarinte aavishkaarathe prathikoolamaayi niyanthrikkunna oru transcription repressor, ithu tumour saprassaraayi pravartthikkumennu soochippikkunnu.
rcor3 (restt corpressor 3): transcription adichamarthunna qoo-repressor complexinte gadakamaayi pravarthichekkaavunna oru protein
thaazhepparayunna vairal proteinukalumaayi ithu edapedunnu 
polyprotein 1ebi (saars-cove 2) ennathinte pakarpp vairal aarenekalude transcription pakarppilum ulppedunna oru multifunctional protein.
protein iii7 (human papillomavirus): koshachakrathilekku shaanthamaaya koshangalude praveshanathe nayikkunnathiloode vairal genome replicationil oru punk vahikkunnu.
uthbhavavum parinaamavum.
eee proteininte ettavum aadyakaala orthologue ekadesham 948 dasalaksham varshangalkk munbu kingdom animaliayile failam placosovayile trichoplex aderencil prathyakshappettu. adutha ettavum vidoora oorthologukal ekadesham 824 dasalaksham varshangalkk munbu failam sinideriyayil kaanapedunnu.
sequence homology.
mipol 1 protein manushyarilum mattu speesheesukalilum ariyappedunna paaralogukalonnumilla, athinaal, athinte jeane kudumbatthile eaka amgamaanithu.
praimettukal muthal pavizhapputukal vareyum failam sinideriyayil kadal anaemonukalum ulppede mrugangalil mipol 1 proteininte 300 ladhikam oorthologukal undu. proteente oorthologukal lalithamaaya aadima akasherukkal ulla oru inamaaya trichoplax aderence pole vidoora enangalil kandethi. pattika 2 orthologue spacinte oru sample kaanikkunnu.
sasthanikal, uragangal, pakshikal, ubhayajeevikal thudangiya kordettukalil 70 sathamaanathiladhikam sequence samaanathakalulla adutha bandhamullah oorthologukal kaanapedunnu. oorthologukalude sequence dairkhyam manushyante mipol 1 proteinu samanamaayirunnu, kaaryamaaya jeane duplication nireekshikkappettilla.
asthi malsyam, cartilaginus malsyam, selakkanthukal ennivayil 55-70% shreniyil (mithamaaya bandhamullah oorthologukal) sequence samaanathakalulla jeevikale kandethi. eee speesheesukalil sequence dairkhyam saadhaaranayaayi kooduthalaanu, enn-terminasil dairkhyameriya amino assid sequence undu (manushya proteinumaayi vinyaasam amino assid 100 nuu chuttum sambhavikkunnu).
50 sathamaanathil thaazhe (ekadesham <aii. dii. 1>) saamyamulla vidoora bandhamullah oorthologukal hemicordateukal, ekkinodermukal, aarthropodukal, molaskkukal, sinidaria, plakosova ennivayil kaanapedunnu. vidoora oorthologukalumaayulla onniladhikam sequence vinyaasam proteente enn-terminasile mosham vinyaasathe soochippikkunnu.
eee proteinil rund cog (proteinukalude orthologas grouppukalude klasttarukal) domainukal kandethi (fig.3 kaanuka): cog1196 106-340 sthaanathu (chromosome segregation atpes), cog4372 259-431 (duff3084 domaine adangiya savisheshathayillaatha samrakshitha protein)
phylogenetics.
vividha mipol 1 oorthologukalkkaayi manushyaril ninnu vyathichalicha theeyathiyude pravartthanamaayi thiruthiya sathamaanam vyathichalanathinte (100 amino aasidukalil amino assid mattangal) oru plottil oru leanier regression vishakalanam upayogichu (fig.5 kaanuka), mipol 1 proteinile amino aasidukalile 1 sathamaanam maattathinu 5.68 dasalaksham varshangal edukkumennu kanakkaakkappedunnu. fibrinogen alfa polulla athivegam vikasichukondirikkunna proteinukalumaayum cytochrome sii polulla pathukke vikasichukondirikkunna proteinukalumaayum thaarathamyappeduthumbol mipol 1 protein mithamaaya nirakkil vikasikkunnu.