
sailos metabolism

vividha jeevikalkk catabolise cheyyaano upayogapradamaaya uthppannangalayi metabolise cheyyaano kazhiyunna anchu kaarban aldos (pentos, monosacride) aanu dii-sailos.
dii-sailosinte kaattabolisathinu kuranjathu naalu vyathyastha paathakalaanullatha eucariotic sookshumaanukkalil oru auxido-reductase paathayund. prokaariyottukal saadhaaranayaayi oru isomerase paatha upayogikkunnu, koodaathe yathaakramam weimberg, dahams pathakal ennu vilikkunna rund auxidative paathakalum procariotic sookshumaanukkalil kaanapedunnu.
vazhikal.
auxido-reductase paatha.
eee paathaye "sailos reductase-sailittol dehydrogenase" allengil xr-xdh pathway ennum vilikkunnu. sailos reductase (xr), sailittol dehydrogenase (xdh) ennivayaanu eee paathayile aadyathe rund ensimeukal. naad allengil naduff upayogichu dii-sailos sailitolaayi kuraykkunnu. cofactor naad upayogichu xdh upayogichu sailittol dii-sileulosaayi ooksidais cheiyunnu. avasana ghattathil dii-silulose kinace, xk upayogichu oru atp phosphorylate cheiyunnu, ithu pentos phoosphett paathayude intermediattaaya dii-silulose-5-phosphettinu kaaranamaakunnu.
isomerase paatha.
eee pathayil sailos isomerase ensime dii-sailosine nerittu dii-sailulosaki maattunnu. auxido-reductase paathayilennapole dii-silulose pinneedu dii-silulose-5-fosfettaayi phosphorylate cheiyunnu. santhulithaavasthayil, isomerase prathikaranam 83 sathamaanam dii-sailos, 17 sathamaanam dii-sailulos ennivayude mishrithathinu kaaranamaakunnu, kaaranam sailosine cylulosilekku parivartthanam cheiyunnathu oorjjaparamaayi prathikoolamaanu.
weimberg paatha.
dii-sailos dehydrajanes vazhi dii-sailos dii-sailono-lactonayi ooksidais cheyyappedunna oru auxidative paathayaan weimberg paatha, thudarnnu lactonine dii-silonic aasidilekku jalavishleshanam cheiyunnathinu oru lactonase. oru sailonettu dehydrates oru jala thanmatra vibhajikkunnu, athinte falamaayi 2-keetto 3-deoxy-sailonettu undaakunnu. 2-keetto-3-deox-dii-sailonettu dehydrate α-kettogloottaarett semieldehyde roopappeduthunnu. ithu pinneedu α-kettogloottaarett semieldehyde dehydrogenase vazhi ooksidais cheyyappedukayum citric assid sykkilile oru pradhana intermediattaayi varthikkunna 2-kettogloottaarett uthpaadippikkukayum cheiyunnu.
dahams paatha.
dahams paatha weimberg paathayaayi aarambikkunnu, ennaal 2-keetto-3 deoxy-sailonettu oru aldolase upayogichu pairuvettu, glycolaldehyde ennivayaayi vibhajikkappedunnu.
jaiva saankethikavidyayude prayogangal.
dii-sailosine ethanolilekku pulippikkunnath abhikaamyamaanu. schifersomises pichiya steeppittis" polulla thaddesheeya sailos pulippikkal yeesttukaliloodeyo allengil "sacharomysus serivisiya" yude metabolikkalaayi enchiniyaring cheytha strainukaliloodeyo ithu saadhyamaakkaam. "pichiya steppittis" yeest uthppaadippikkunna parambaraagatha ethanol "sacharomysus serivisiya" pole ethanol sahishnuthayullathalla. "ezz. maruvashathu serivisiyaykk dii-sailosine ethanolilekku pulippikkaan kazhiyilla. kall" srishtikkaanulla sramangalil. serivisiya "p-yude" xyl1 "," xyl2 "jeenukale dii-sailos pulippikkaan kazhiyunna strainukal. dii-sailos reductase (exr), sailittol dehydrogenase (exh) ennivaykkulla coding yathaakramam essil avatharippichu. janithaka enchiniyaring vazhi serivisiya. xr, d-sailosil ninnu sailitolinteyum xdh sailittolil ninnu d-sailitolinteyum roopeekaranathe uthejippikkunnu. "sacharomysus serivisiya" kku pentos phoosphett paathayiloode swaabhaavikamaayi dii-sailoolosine pulippikkaan kazhiyum.
mattoru sameepanathil, baakteeriyal sailos isomeraseukal eee samvidaanangalil avatharippichittundu. serivisiye ". eee ensime dii-sailosil ninnu dii-sailoolosinte nerittulla roopeekaranathe uthejippikkunnu. baakteeriyal isomeraseukal prakadippikkaanulla pala sramangalum thettaaya madakkal allengil mattu prasnangal kaaranam vijayichilla, ennaal vaayurahita fungus "pyromyses" ezz. pii. yill ninnulla sailos isomerase. phalapradamaanennu thelinjittundu. oru aanukoolyam avakashappettu. sailos isomerase upayogichu roopakalppana cheytha serivisiya parinamaparamaaya poruthappedutthalinu sesham sailosil anerobicaayi valaraan kazhiyumennathaanu.
dii-sailos mettabolisathinidayil auxidative pentos phoosphett paathayiloode ozhukunnathinekkurichu padanangal eee ghattatthinte nirakk parimithappeduthunnathu ethanolilekkulla pulippikkalinte kaaryakshamathaykk gunam cheiyumennu velippeduthi. ethanol uthppaadanam mechappeduthiyekkaannu eee flexile parishkkaranangalil jii. enn. dii 1 jeane allengil isad. dablyu. eph 1 jeane illaathaakkunnathu ulppedunnu. mettabolisatthinte samayat pentos phoosphett paatha adhika naduff uthppaadippikkunnathil, eee ghattam parimithappeduthunnathu naad (pii) echu, naad + coffactorukal thammilulla ithinakam vyakthamaaya asanthulithaavastha pariharikkanum sailittol upolppanna roopeekaranam kuraykkaanum sahayikkum.
rund dii-sailos metabolising paathakale thaarathamyam cheytha mattoru pareekshanam velippeduthi, ettavum valia ethanol vilavu uthppaadippikkunnathina dii-sailosine metabolise cheyyaan xi paathaykk mikacha kazhivundennu velippeduthi, athesamayam xr-xdh paatha ethanol ulppaadanathinte valare vegathayeriya nirakkil ethi.
noonn-auxidative pentos phoosphett pathway ensaimukalaaya transaldolase, transkettoles, ribulose-5-phoosphett epimerase, ribos-5-phoosphett kettol-isomerase enniva encode cheiyunna naalu jeenukalude amithamaya aavishkaaram uyarnna dii-sailulos, dii-sailos pulippikkal nirakkilekku nayichu.
ethanol kaaryakshamamaayi uthppaadippikkunna oru yeest strain vikasippikkuka ennathaanu laborattariyile eee janithaka punasamyojanatthinte lakshyam. ennirunnaalum, dii-sailos metabolising laborattari strainukalude falaprapti ellaayppozhum prakrithiyile asamskritha sailos uthppannangalil avayude metabolism kazhivukale prathifalippikkunnilla. maram polulla kaarshika avashishtangalil ninnaan dii-sailos kooduthalum verthirikkappedunnathil, thaddesheeyamo janithakamattam varuthiyatho aaya yeest eee shudhamallaatha prakrithi srothassukale upapachayam cheiyunnathil phalapradamaayirikkanam.
dii-sailos metabolism paathayude karyakshamatha opttimais cheyyaanulla shramathil xr, xdh ensaimukalude alavinte vyathyasthamaaya aavishkaaram laboratariyil pareekshichittundu.