
thymet oligopeptidase

tops ennum ariyappedunna thymet oligopeptidesukal (θιπετοπτιδας) oru tharam emm3 metalopeptidesukalaanu. eee ensimeukal mrugangalilum sasyangalilum kaanapedunnu, ithu vyathirikthamaya pravartthanangal kaanikkunnu. mrugangalilum manushyarilum, bradikinin, neuro tensin, angiotensin aii, aba pepttaid thudangiya pepttaidukalude apachayathil avaa ulppedunnu, ithu physiological prakriyakale niyantrikkaan sahayikkunnu. sasyangalil, avayude punk targettucheyyunna pepttaidukalude apachayavum salicylic assid (sa)-ashrita stress signalingiloode rogakaarikalodulla rogaprathirodha prathikaranavumayi bandhappettirikkunnu. shaasthreeya padanangalkkulla oru maathrukaa sasyamaayi amgeekarikkappetta "arabidopsis taliana" yill-topp 1, topp 2 ennariyappedunna rund thymet oligopeptidesukal chediyile salicylic assid bindinginte lakshyangalayi thiricharinjittundu. vividha gadakangalumaayi edapedunnathum mikka paathakalum anjjaathavumaaya zaa-mediated signalling manasilakkunnathinulla pradhana gadakangalaanu eee topp ensimeukal.
uthbhavavum perum.
raajya mrugangalil thymet ("theolsensitive mettalo" yill ninnu) oligopeptidesukal (pepttaid-sais niyanthranam) vyaapakamaayi padikkappettittund. aadyathe topp ensime 1983 ll elikalude mastishka homogenateukalil ninnu kandethukayum shudheekarikkukayum cheythu. innu, topp ensimeukal kooduthalum manushyarudeyum elikaludeyum pityoottari, masthishkam, vrishanangal ennivayilaanu vitharanam cheyyappedunnathu. sasyangalil, prathyekichu "eiii" yill. taliana, "20-kalile protesominteyum zaa-binding proteinukaludeyum bhaagamaayi ensimeukal aduthide kandethi.
layikkunna metalloproteinase, pii. isad-peptidays, collagenase polulla peptidays, thimett peptidays, endopeptidase <aii. dii. 1> thudangiya eee koottam ensaimukale thirichariyaan vyathyastha perukal upayogichittundu. 1992-ll internaashanal union oof biochemistry aandu molecular biology (aii. yuu. bii. emm. bii) eee peru samrakshichukondu "thimett oligopeptidase" enna peru nirddeshichu.
kudumbam.
maa, sabklan maa (iii), emm3 kudumbam, emm3eiii upakudumbam ennivayil ulppedunna metalopeptidesukalaanu thymet oligopeptidesukal. neurolysine ("iii. sii" <aii. dii. 2>), sacarolysine ("iii. sii": <aii. dii. 1>), oligopeptidase mepb, oligopeptidase eiii, oligopeptidase eph, oligopeptidase pepb thudangiya ettu amgangalaanu emm3 kudumbatthil ulppedunnathu. eee ensimeukal procariotic, eucariotic jeevikalil pradhana pravartthanangal nadathunnu.
"arabidopsis" thymet oligopeptidesukal.
arabidopsis taliana" yill moonnu tharam toppukal kaanapedunnu. topp 1 (oopu, oorganellar oligopeptidase, topporg, thymet metaloendopeptidase 1 ennum ariyappedunnu) mitocondriyayilum chloroplastukalilum sthithicheyyunnu, ithinu 565620 enna jeane undu. topp 2 (thymet metaloendopeptidase 2 ennum ariyappedunnu) cytosolil sthithicheyyunnu, ithinu eiii. tii. 5jii10540 jeane undu. eee rund ensaimukaludeyum vyathirikthamaya jeenukal pazha eecha, kothukukal, arrie ennivayilum nilanirthunnu. avasaanamaayi, topple (topp-like ennum ariyappedunnu) cytosolil sthithicheyyunnu, ithinu eiii. tii. 1jii. 67690 jeane undu.
eee toppukal sasthanikalude tissuekalil kaanappedunnathin samaanamaan. topp 1 umm topp 2 umm amino assid shreniyil 28 sathamaanam saamyamulla mouse topinode kooduthal saamyamullavayaan, athesamayam topp amino assid shreniyil 34 sathamaanam saamyamulla mouse neurolysinumaayi kooduthal saamyamullathaan.
jaiva raasa savisheshathakal.
amino assid shreniyil (2014 leeii vaangil udharichathupole) samrakshitha sreniyaaya hiss-gloo-x-x-hiss ulla emm3 peptidesukalaanu thymet oligopeptidesukal. ensaimukalkku avayude pravartthanangal nirvahikkaan thayolinteyum sinkinteyum saannidhyam aavasyamaanu. a" yill thirichariyaan kazhiyunna moonnu thymet oligopeptidesukalil ninnu. taliana, "topp 1, topp 2 enniva maathramaan rogaprathirodha samvidaanathinte prathikaranatthil ulppedunna topp ensimeukal. 6.5 muthal 8.5 vareyulla vishalamaaya piechu shreniyil avaykku avarude pravartthanam nadatthaan kazhiyum. topp ensaimukalkku oru diamer bandhippikkanum roopappeduthaanumulla sheshiyund. avaa monomerukalaayum dimerukalaayum nilanilkkaam.
protein shreniyil 93 sathamaanam saamyamulla topp 1, topp 2 enniva homologukalaayi kanakkakkunnu. avarude gadanakalkku rund domainukalundu. ennirunnaalum, domaine i-leeii helixukal α6, α9, α20 ennivayude sthaanangalile vyathyaasangal kaaranam eee domainukal topp 2-nekkaal topp 1-ll aduthaanu. athukondaanu topp 1 nuu clossed foam topp 2 nuu ooppan foam ullathu. topp 1 ll ninnu vyathyasthamayi, topp 2-ll athinte shreniyil enn-terminal serin sambannamaaya oru pradesham adangiyittilla. koshatthinullile praadeshikavalkkaranathe adisthaanamaakki, topp 1 eratta praadesikavalkkaranamulla oru oorganellar oligopeptidesaayi kanakkaakkappedunnu, topp 2 nuu cytosolic oligopeptidase ennu namakaranam cheiyunnu.
sasyangalile pravartthanangalum edapedalukalum.
pepttaid apachayam.
mitocondriyayilekkum chloroplastukalilekkum proteinukal irakkumathi cheyyumbol anaavashya pepttaidukalude apachayathinulla pathayil topp 1 oru punk vahikkunnu. topp 1 gadanayude clossed konformationile mattangal kaaranam eee function vishadeekarikkaan kazhiyum. I, II domainukal srishtikkunna spheroidal aakruthi, 3,000 Â3 voliyamulla kaattalitik araye prathinidheekarikkunnu. pepttaid degradation samayat, rund domainukalum verthirikkappettal maathrame substrate binding sambhavikkoo, ithu arayilekku (active saitt) swatantra targetting pepttaidukale axus cheyyaanum bandhippikkanum anuvadikkunnu. hydrophobic, dhruva edapedalukal enningane niravadhi noonn-kovalant edapedalukaliloodeyaanu ensime substrate complexes paripaalikkappedunnathu. pepttaidinte valippam eee prakriya parimithappedutthunnu. athinaal, ekadesham ore alavilulla (3,000 Â3) pepttaidukale maathrame ensaiminu tharamthaazhthaanaakoo. vichedikkaavunna pepttaidukal 8 muthal 23 vare amino assid avashishtangalkidayilaanu.
koodaathe, mattu tharatthilulla pepttaidukalude apachayathilaanu topp 1 sambhavikkunnathenn nirddeshikkappettittund. mitocondria, chloroplastukal ennivaykkullil thurannatho kedupaadukal sambhavichatho aaya proteinukal illaathaakkunnathinu ensime pradhaanamaan. proteinukalude pilarpp prakriyayil, ensaiminu pepttaid shakalangale karsanamaaya sequence aavasyamillaathe takarkkaan kazhiyum, pakshe pepttaidinte valuppam kondu maathram parimithappedutthunnu.
cytosolil nadakkunna proteoletic pravartthanatthinte bhagamaanu topp 2. eiii. 5 muthal 17 vare amino assid avashishtangalkkidayil pepttaidukale thakarkkunnathiloode auxidative sammarddathodum ooksidaisd proteinukalude apachayathodumulla prathikaranatthil taliana enna ensaiminu oru pankundu.
stress signalling prathirodhasheshi prathikaranavum.
oru phenolic samyukthamaayi amgeekarikkappetta salicylic assid (ezz. eiii) sasyangalkkullile vividha jaiva prakriyakalil ulppedunnu. eee samyuktham mattu hormonukalumaayi chernnu valarchaykkum vikasana niyanthranathinum sambhavana cheiyunnu. koodaathe, avaa sasyangalude rogaprathirodha prathikaranam nilanirthunnu. rogakaarikalude akramanangaleyum sammarddaavasthakaleyum prathirodhikkunnathin, ezz. eiii signalling rogakariyaaya triggard immunity (pidiai), ifakttar triggard immunity (idiai), prograam cheytha sell deth (pcd) enniva sajeevamaakkunnu. topp 1, topp 2 enniva salicylic aasidumaayi samvadikkaanum sisttathile sa pravarthanatthinu madhyasthatha vahikkanumulla kazhivu kaanichittundu. "eee ensimeukal illaathirikkukayo thakararilavukayo cheyyumbol rogakarikalaaya anubaadhaykkulla saadhyatha kooduthalaanennu inn vitro pareekshanangal theliyichittundu.
tops proteolytic pravartthanatthe avayude monomer/diamer bandham badhikkunnu. auxidative paristhithi diamer roopam vardhippikkukayum paristhithi kuraykkumbol proteolytic pravartthana nirodhanathinu kaaranamaakukayum monomerukalude shekharanathe uthejippikkukayum thudarnnu topp ensaimukalude pravartthanatthe anukoolikkukayum cheiyunnu. sammarddaavasthayil, reactive ooksijan speesheesukalude (rooss) uthppaadanam vardhikkukayum prakaashasamshleshanathilum pcdyilum naashanashtangal undaakkukayum cheiyunnu. eee prathikoolamaaya avasthayodulla prathikaranatthil topp ensimeukal madhyasthatha vahikkunnu. topp 1 chloroplastilekk aantiooksidant ensaimukalude irakkumathi niyanthrikkunnu. eee aantiooksidant ensimeukal oorganellinullile rooss alavu kuraykkunnu. zaa sinthasisine uthejippikkunna ensaimukalude irakkumathi topp 1 anuvadikkunnuvennum nirddeshikkappettittund. maruvashathu, topp 2 cytosolile pii. sii. dick pradhanappetta signalling pepttaidukalude alavu niyanthrikkunnu. cadmium (sidi2 +) stress polulla auxidative kedupaadukal koshatthile kooduthal kedupaadukal thadayunnathinu neekkam cheyyenda ooksidaisd proteinukal uthpaadippikkunnu. topp 2 eee kedupaadukal sambhavicha proteinukalude apachayathinu sahayikkunnu. sammarddasamayathu thurannukaattappedunna amino assid avashishtangale lakshyamit ensime pepttaidukale tharamthaazhthunnu.
exogenous salicylic assid thymet oligopeptidesukalum thammilulla edapedal.
sasyangalil, endogenous zaa aaktiveshan exogenous zaa vazhi nadatthaam. 10 micrometeril thazheyaayi kanakkaakkappedunna exogenous zaa yude kuranja alavu, ajaiva sammarddam moolamundaakunna kedupaadukal kuraykkaanum prakaashasamshleshana pravartthanam vardhippikkaanum sahayikkunnu. ithinu vipareethamaayi, uyarnna alavilulla exogenous zaa prakaashasamshleshana pravartthanam kuraykkukayum koshangalude maranathinu kaaranamaakukayum cheiyunnu. eee uyarnna alavilulla exogenous zaa topp ensaimukalumaayi samvadikkukayum avayude ensaimatik pravartthanam kuraykkukayum cheiyunnu. 5 dasalaksham exogenous zaa yude saannidhyathil topp 1, topp 2 pravartthanangal ekadesham 60 sathamaanam kuranju. eee phenolic samyuktham sajeevamaaya saittil ninnu vyathyasthamaaya oru saittil zaa bandhippikkunna oru malsaraathmakamallaatha samvidaanathiloode topp ensaimukale thadayunnu. prathikaranatthinte tharam paramavadhi vegatha (vii) kurayunnathinte savisheshathayaanu, athesamayam michaelis sthiraangam (keey) maattamillathe nilanirthunnu.

