
aarr. enn. eiii-binding protein daattaabes

aarr. enn. eiii-binding protein daattaabes (aarr. bii. pii. dii. bii) aarr. enn. eiii-binding saittukalude pareekshanathmaka nireekshanangal ulppedunna aarr. enn. eiii-binding protein prathyekathakalude oru jaiva daattaabesaan. praadhamika saahithyathil ninnulla inn vitro, inn vivo ennivayaanu ulppeduthiyittulla pareekshanathmaka phalangal. homo sapiens", "musculus", "drosophila melanogaster", "synorhabditis elegans" ennee naalu mettasovan enangal ithil ulppedunnu. eee daattaabesil ulppeduthiyittulla rna-binding domainukal rna recognission motif, keey homology, sisich sink finger ennivayum athilereyum domainukalumaanu. ettavum puthiya aarr. bii. pii. dii. bii. releasaaya (vii1.3, septambar 2012) ll 1,171 aarr. enn. eiii-binding proteinukal ulppedunnu.
aarr. enn. eiii binding proteenekkurichulla pashchaathala vivarangal.
prokaariyottukalilum yookkaariyottukalilum transcription vivarthana prakriyakalum vyathyasthamaan. prokaariyottukalil ninnu vyathyasthamayi, eee rund prakriyakalum yookkaariyottinte nucleusilum cytoplaasathilum vevvere sambhavikkunnu. ekkaaranathaal, yookkariyottukal pree-marnah prosess cheiyunnathinu spliesing, editting, polyadenylation enniva ulppedunna poost-transcription modification enna thanthram prayogikkunnu. eee prakriyayil aarr. enn. eiii-binding proteinukal (aarr. bii. pii. ezz) nirnaayaka punk vahikkunnu. ellah aarr. bii. pii. esinum aarr. enn. eiii. yumayi bandhippikkaan kazhiyum, ithu vyathyastha prathyekathakaleyum bandhangaleyum aasrayichirikkunnu. aarr. bii. pii. esil kuranjathu oru aarr. enn. eiii-binding domainukalenkilum adangiyirikkunnu, saadhaaranayaayi avaykku onniladhikam binding domainukalundu. rna-binding domaine (rnp domaine, rna recognission motif, rrm ennum ariyappedunna arbidi), keey-homology (kha) domaine (type aii, type II), aarji (aarji-gly-gly) boxes, sm domaine; dedd/deeh boxes, sink finger (znf, kooduthalum sii-x8-x-x5-x-x3-h), double strended rna-binding domaine (dsrbd), colled-shock domaine; pumilio/efbf (puf allengil pumm-echdi) domaine, pivai/argonuit/swille (paas) domaine enniva nannayi savisheshathakalullathaanu.
onniladhikam binding domainukalaanu aarr. bii. pii. ezz nirmmikkunnath. eee domainukalil kurachu adisthaana modular unitukal adangiyirikkunnu. orotta roopavumaayi thaarathamyappeduthumbol, aarr. bii. pii. esinu aaa onniladhikam roopangal upayogichu nucleic aasidukalude valare dairkhyameriya stretch thirichariyaan kazhiyum. athesamayam, aarr. bii. pii. ezz durbalamaaya edapedalukal roopappeduthikkondu aarr. enn. eiii. yumayi bandhippikkunnu. durbalamaaya prathipravartthana uparithalam pradhaanamaayum eee roopangalaal vardhikkunnu. thalphalamaayi, aarr. bii. pii. esinu aarr. enn. eye orotta domaininekkaal uyarnna prathyekathayumaayum aduppathodeyum bandhippikkaan kazhiyum. aarr. enn. eiii-binding protein daattaabesin moonnu pradhana prathyeka vibhaagangalund. avaa rna recognission motif (rrm), keey-homology domaine (keey domaine), sink viralukal ennivayaanu.
bandhapetta gaveshanam.
aarr. enn. eiii-binding protein domainukal.
lundeyude lekhanathil, avarude samgham vividha tharam aarr. enn. eiii-binding protein roopavum avayude prathyeka pravartthanangalum avatharippichu.
aarr. enn. eiii. recognission rotif (aarr. aarr. emm).
rna recognission rotifil (rrm) ekadesham 80-90 amino aasidukal adangiyirikkunnu, avaa rund helixukalulla (βαββαβ topology) naalu-stranded aanti-paaralal β-sheett undaakkunnu. rna thiricharivinaayi β-sheett nirnaayaka punk vahikkunnu. saadhaaranayaayi, β-sheettile moonnu samrakshitha avashishtangal eee thirichariyal prakriyaykku valare pradhaanamaan. prathyekichum, oru aarg allengil lies avashishtangal phosphodeestarinte nattellilekku oru uppu paalam undaakkunnu, koodaathe mattu rund sugandha avashishtangal nucleobaseukalumaayi adukki vaykkunnu. eee naalu β-sheettukalil oronnum oru nucleodideukale thirichariyunnu. ennirunnaalum, thuranna looppukalum adhika dvitheeya ghadanayum upayogichu, rrminu 8 nucleotideukal vare thirichariyaan kazhiyum.
keey-homology domaine (keey. echu domaine).
keey-homology domaine (keey. echu domaine) aanu manushyanil aadyamaayi thiricharinjathu. ithu heterogeneous nuclear raibo nucleoprotein (hnrnp) keey yill ninnaan. athinaal, eee kudumbatthil pedunna binding domainukale keey-homology domaine ennu vilikkunnu. ithu ssdna, ssrna ennivayumaayi bandhippikkunna oru domainanu. yookkariyottukal, eubacteriakal, archaea ennivaykku saadhaaranayaayi itharathilulla domainukalundu. eee domainil ekadesham 70 amino aasidukal adangiyirikkunnu. eee domaininte pradhana signature sequence (i/l/v) igxxgx (i/l/v) aanu. ellah keey. echu domainukalilum moonnu-stranded β-sheettum moonnu α-helixukalum adangiyirikkunnu. eee domaine rund upakudumbangalundu. type aii keey domaine (βαββα topology), type II keey domaine (αββαβ topology). rund classukalkkum, gxxg lupe, flanking helisukal, β-strand, β2 num β3 num (type i) edayilulla allengil α2 num β2 num (type II) edayilulla variable lupe enniva rna thirichariyunnathil valare pradhanappetta punk vahikkunnu.
sink viralukal.
sink ekopitha avashishtangal adangiyirikkunna domainukalaanu sink viralukal. eee domaine moonnu pradhana tharangalundu, avaa cys2his2 (cchh), ccch allengil cchc aanu. saadhaaranayaayi, eee domaininte niravadhi aavarthanangal oru proteinil orumichu pravartthikkunnu. ch sink viral dnaumayi bandhippikkumbol, athinte amgeekaarathile avashishtangal α-helix pradhana groovil vaatsan-crick bays jodikalumaayi hydrajan bondukal roopappeduthunnu. ithu rnaumaayi bandhippikkumbol, dna thirichariyaan upayogikkunna athe avashishtangal ippozhum rna thirichariyaan upayogikkaam. eee rund tharam nucleodideukaleyum verthirichariyaan sink figure upayogikkunna thanthrathil eee domaininte vyathyasthamaaya gadanaaparamaaya krameekaranam adangiyirikkaam. sii. sii. sii, sii. sii. sii sink viralukal ooo. yuu. sambushtamaaya aarr. enn. eiii moolakavumaayi bandhippikkunnu. sitch sink aakruthiyil ninnu vyathyasthamayi, proteente aakruthiyaanu prathyekathayude praadhamika nirnnayaka ghadakam.
aarr. enn. eiii-binding proteente sequence munganana.
reyudeyum kasanudeyum pepparil, aarr. bii. pii. esinte sequence mungananayekkurichulla chodyathe avar abhisambodhana cheiyunnu. avarude gaveshanathil, orotta aarr. bii. pii. ykku sankeernamaaya aarr. enn. eiii. kalude oru valia mollar adhikamaayi incubate cheyyappedunnu. affinity selection vazhi protein veendedukkukayum anubandha aarenekale microare, computational vishakalanangal enniva vazhi chodyam cheyyukayum cheiyunnu. rna-binding proteinukalkku sequence mungananayundennum samaanamo adutha bandhamullatho aaya arbps samanamaaya rna seekwansumaayi bandhippikkumennum avayude phalangal kaanikkunnu.
upayogikkuka.
ippol, aarr. enn. eiii-binding protein daattaabesil (aarr. bii. pii. dii. bii) "homo sapiens", "musculus", "drosophila melanogaster", "synorhabditis elegans" ennivayil ninnulla 1171 aarr. enn. eiii-binding proteinukal adangiyirikkunnu. proteinukal domaine allengil speesheesukal anusarichu tirayan kazhiyum. jeane chihnam, vyaakhyaana aidi, paryaayapadangal, jeane vivaranam, speeshees, rna-binding domaine, pareekshanangalude ennam, homologukal enniva ulppedunna proteinukalude vishadamaaya vivara pattikayilekku rund vazhikalum nayikkum. pareekshanangalude annathekkurichulla link proteinumaayi bandhapetta gaveshana lekhanangalilekku nayikkunnu. koodaathe, eee daattaabesil upayokthaakkalkk nirddishta rna binding seekwansumaayi bandhapetta pareekshanangal tirayan kazhiyum. koodaathe, oru seekwansinaayi binding sittukal pravachikkaan eee saittin upayokthaakkale sahayikkaanaakum.

