aars oopparan

molecular biologyil, niravadhi bacteria taxonukalil kaanapedunna oru oopparan aanu aars oopparan. aarsenett, aarsenait, antimonite ennivayude vishaamsham illaathaakkunnathinu ithu aavasyamaanu. eee samvidaanam koshatthil ninnu arsenite antimonite puratthekku kondupookunnu. arsa, arsb jeenukalude ulppannangalaaya rund polypeptideukal chernnathaanu pump. eee rund-subunit ensime arsenitinum antimonitinum prathirodham srishtikkunnu. ennirunnaalum, aarsenett puranthallunnathinu munbu athu aarsenaittayi kuraykkanam. moonnaamathe jeane, rsc, aarsenett pumping prathirodhavum anuvadikkunnathinu substrate nirddishtatha vikasippikkunnu. arsk ekadesham 150 avashishtangalulla aarsenett reductase aanu, ithu sajeevamaaya saittil oru redox sajeeva sisttain avashishtathode aarsenettine aarsenaittayi parivartthanam cheyyaan reduced glutathiyon (jii. ezz. echu) upayogikkunnu. jii. ezz. echu, aarsenett, gluteredoxin 1 (jii. aarr. ex. 1) ennivayulla oru sajeeva kwaattenari complexes aarr. ezz. sii roopeekarikkunnu. kuravu sambhavikkunnathin moonnu ligandukalum oresamayam undaayirikkanam.
arsa arsb.
arsa arsb oru ayoon-translotting atpes roopappeduthunnu. membranumaayi bandhapetta chaanal enna nilayil athinte punk kanakkileduthu arsb protein athinte mothatthilulla hydrophobic swabhaavathaal verthirichirikkunnu. sequence vishakalanam 13 anumanaparamaaya transmembran (tm) pradeshangalude saannidhyam velippeduthunnu.
aarr. ezz. sii.
arsk protein ghadana pariharichu. alfa-helikkukalaal chuttapetta beetaa-sheett coor nirvachichirikkunna theoredoxin suuppar famili foldil pedunna onnaanith. aarsinte sajeevamaaya sisttain avashishtangal aadyathe beetaa-strandinum aadyathe helixinum edayilulla looppilaan sthithicheyyunnathu, ithu ezz. pii. ex proteinilum athinte homologukalilum samrakshikkappedunnu.
disulfide sammarddathodulla prathikaranamaayi onniladhikam jeenukalude transcriptione niyanthrikkunna gram positteev bacteria transcription gadakangalaaya ezz. pii. ex proteinukalum aarr. ezz. sii kudumbatthil ulppedunnu.
rsd, rsr.
escherichiya koliyil" arsenicalukalkkum antimoniyalukalkkum prathirodham nalkunna arsardabk oopparaninte trans-acting repressoraanu rsd. ithil rund jody visinal sisttain avashishtangal undu, sis (12)-sis (13), sis (112)-sis (113), avaa metaloyidukalkkaayi prathyeka binding sittukal undaakkunnu, ithu oopparanil ninnu arsd vighadanathinu kaaranamaakunnu. ennirunnaalum, oru homodymer enna nilayil ithinu naalu visinal sisttain jodikalundu. rsd kudumbatthil niravadhi baakteeriyal arsenical resistans oopparan trans-acting repressor rsd proteinukal adangiyirikkunnu.
trans acting regulatory proteinaan rsr. koshatthil arsenic illaathirikkumbol ithu arsardabk oopparanil oru adichamarthal yanthramaayi pravartthikkunnu. koshatthil arsenic undaakumbol aarsaarinu oopparettarumaayulla aduppam nashtappedum, koodaathe rna polymerasinu arsdcabnte jeenukal transcribe cheyyaan kazhiyum.
rs operon niyantrikkaan arsd rsr orumichu pravartthikkunnu.
arsenic chaaperon, rsd, escherichiya koliyude arsardabk oopparan encode cheythathaanu. rsd trival metaloyidukale aarsayilekk maattunnu, ithu oru (iii)/esby (iii) efflex pambinte kaattalatik sabyoonittaan. arsdumaayulla edapedal arsenitinodulla aarsayude aduppam vardhippikkunnu, angane aarsenaitinte kuranja saandrathayil athinte atpes pravartthanam vardhippikkukayum aarsenait puranthallalinte nirakk vardhippikkukayum cheiyunnu.

