
4piai sted microscopy

uthejippicha emission depletion (sted) microscopy, 4piai-microscopy ennee bandhamillaattha rund ashayangal samyojippichathinte falamaanu 4piai-sted-microscope. ivide, rund ethir lensukalude pothuvaaya focusil oru fluorescent sample sthaapichittundenkil eksaitteshanum ditakshanum orotta lensiloodeyaanu (4piai mooad eiii) nadathunnathu. pacha eksaitteshan pulsine udan thanne oru chuvanna sted-pulse pinthudarunnu, ithu rund lensukaliloodeyum phokkal mekhalayilekku praveshikkunnu, ithu uthejithamaaya fluorescent thanmaathrakalude uttejaka udvamanam nilathekku prerippikkunnu. kendratthil ninnulla fluorescence emission anuvadikkunnathinum ennaal ayal pradeshangalil ninnu athine adichamarthunnathinum sted beam kendratthil kuranjathu aayirikkan ghattam ghattamayi maattunnath upayogapradamaanu.

