
ezz. bii. buffer

dna, rna thudangiya nucleic aasidukale verthirikkunnathin agaros, polyacrylamide jell electrophoresis ennivayil upayogikkunna oru buffer layaniyaan esby buffer. "brodyudeyum kerninteyum prasidheekaranangale adisthaanamaakkiyulla soodiyam boric assid adisthaanamaakkiyulla chaalaka maadhyamathinu (us pettant #7811437) vegathayeriya mikacha media ellcyude vaanijya vyaapaaramudrayaanu "esby".
ithu soodiyam borettu kondaanu nirmmichirikkunnathu, saadhaaranayaayi piechu 8 ll 1-10 emm. ithinu kuranja chaalakathayund, moorchayulla baandukal uthpaadippikkunnu, koodaathe tibe buffer allengil tay buffer (5-10 vii/centimeterumaayi thaarathamyappeduthumbol 5-35 vii/centimeter) upayogichu nirmmicha jellukalekkaal uyarnna vegathayil pravarthippikkaan kazhiyum. oru nischitha voltageil, choodu srishtikkappedukayum angane jell choodaakkukayum cheyyum. ennirunnaalum, esby bufferinu tibi, taay ennivayekkaal kuranja chaalakathayund, athinaal jell thaapanila tibi allengil taay bafarukalekkaal valare kuravaanu. athinaal, electrophoresis vegathilaakkaan volteju vardhippikkaan kazhiyum, angane oru jell rann saadhaarana samayathinte oru bhagam maathrame edukkoo. jell sliceil ninnu dna verthirichedukkal allengil sathen bloat vishakalanam polulla thaazhekkulla prayogangal soodiyam borettu jellukalil pratheekshichathupole pravartthikkunnu.
lythium borettu adangiya elby buffer soodiyam borettinu samaanamaan, athinte ellah gunangalumundu, ennaal soodiyam ayonukalumaayi thaarathamyappeduthumbol lythium ayonukalude chalakatha kuravaayathinaal uyarnna voltageukal upayogikkaan anuvadikkunnu. ennirunnaalum, lythium borettinu alppam kooduthal chelaveriyathaanu.

