
roose sarcoma vairas

raus sarcoma vairas (aarr. ezz. vii) () oru retrovirusanu, ithu vivaricha aadyathe oongovairasaanu. ithu kozhikalil sarcomic kaaranamaakunnu.
ellah retrovirusukaleyum pole, hoost dnaumayi samyojippikkunnathin munbu ithu athinte rna genome cdnaylake transcribe cheiyunnu.
charithram.
newyork nagaratthile rockfeller sarvakalashaalayil jolie cheiyunna paittan roose 1911 ll aarogyakaramaya plymouth roock kozhikalil chikkan tumourinte kosharahita sathil kuthivachu rsv kandethi. eee sathil ninnu oongoojenisis undakunnathaayi kandethi. connective tissue (oru sarcoma) kondaanu tumour nirmmichathennu kandethi. angane, kaansarinte valarchaye thanmaathraaparamaayi padikkaan upayogikkaavunna aadyathe oongoojenik retrovirus ennaanu aarr. ezz. vii ariyappedunnathu.
1958-ll haari rubin howard temin chernnu chikkan bhroona fibroblastukale rsv anubaadhayiloode roopashaasthraparamaayi maattaan kazhiyunna oru parisodhana vikasippichu. rund varshathinu sesham koshangalude parivartthanam cheyyappetta roopaghadana niyanthrikkunnath arsvyude janithaka savisheshathayaanu ennu temin nigamanam cheythu. akkaalat ithu anjjaathamaayirunnu, ennaal aarogyakaramaya koshangalile roopaghadana parivarthanatthinu utharavaadiyaaya "src" jeane pinneedu thiricharinju. 1960kalil, rund kandethalukal uyarnnuvannu replication-kazhivulla otappetta virusukal aarsviyumaayi bandhappettirunnuvenkum parivarthanamillaathavayinnu, koodaathe arsvyude otappetta replication-defective strain parivartthana-kazhivullathaayirunnu. vairal replication marakamaaya parivartthanavum aaresviyile vyathyastha prakriyakalaanenna daaranaykku eee rund kandethalukalum kaaranamaayi.
1966ll addehathinte kandethalinte praadhaanyathinu shareerashaasthrathino vaidyashaasthrathino ulla nobal sammanam roosinu labhichu. thudarnnu, epsteen-baar vairas polulla mattu oongoojenik human virusukal kandethi. koodaathe, thudakkathil retrovirusukalilum pinneedu koshangalilum oongoojinukal kandethi.
ghadanayum genome.
dna intermediattulla positteev sense rna genome ulla klaas VI enveloped vairasaanu rsv.
arswick naalu jeenukalunda
rsv genome athidhikalkkulla genome samyojanam saadhyamaakkukayum rsv jeenukalude amithamaya aavishkaaram saadhyamaakkukayum cheiyunna terminal aavarthanangalund.
src jeane.
asaadhaaranamaaya aathidheya koshangalil aniyanthrithamaaya valarchaykku kaaranamakunnathinaal src jeane oongoojenik aanu. kandethiya aadyathe retroviral oongoojen aayirunnu ithu. mrugangalude raajyathudaneelam kaanapedunna oru jeane aanu ithu, speesheesukalkkidayil uyarnna alavilulla samrakshanamundu.
src jeane rsv ettedukkukayum athinte genome ulppeduthukayum cheythu, ithu hoost koshangalude aniyanthrithamaaya maittosis uthejippikkaan kazhiyumenna nettam nalkukayum puthiya anubaadhaykk samriddhamaaya koshangal nalkukayum cheiyunnu.
rsv vyaapanathin src jeane anivaaryamallengilum athu undaakumbol viralence valareyadhikam vardhippikkunnu.
koshavalarchayum vyathyasavum niyanthrikkunnathil earppettirikkunna oru tyrosine kinace aanu src. ithinu oru shhaa2, shhaa3 domainukalundu, avaa athinte sajeevamakkalinum nirjeevamakkalinum utharavaadikalaanu.
aarr. enn. eiii. dvitheeya ghadana.
arsvyude rna genome 5 muthal 7 kebi vare neelamulla valare neelamulla 3 'utr adangiyirikkunnu, ithu saadhaaranayaayi eucariotic hoost cellinullile asambandha madhyastha kshayam (enmd) yilek nayikkum. 3 'uthru ullil oru samrakshitha dvitheeya ghadana ghadakam thiricharinjittundu, ithu roose sarcoma vairas stebility ghadakam (aarr. ezz. iii) ennariyappedunnu. eee ghadakam splis cheyyaatha vairal rnaude apachayathe thadayumennu theliyikkappettittund.
roose sarcoma vairasinte genome aanu aarr. ezz. iii moolakathe aadyamaayi thiricharinjathenkilum eviyan retrovirus kudumbatthiludaneelam ithu vyaapakamaayi samrakshikkappedunnu. rse ghadakam ~ 300 bipi neelamullathum gagg naachural translation termination codoninte thazheyaayi sthithicheyyunnathumaanu. aarr. ezz. iii moolakatthinte dvitheeya ghadana nirnnayikkunnath aarr. enn. eiii. ezz. dahanavum roopaghadana rasatantra vishakalanavumaan.
arsvyil thiricharinja mattu gadakangalil primer binding saitt ulppedunnu.
gagg protein.
virian assemblic aathidheya koshatthinte pakwathayulla vairas anubaadhaykkum gagg proteinukal aavasyamaanu. arsvyude gagg proteinil (pr76) 701 amino aasidukal adangiyirikkunnu. ithu vairas encode cheytha protees upayogichu pilarnnu pakarchavyaadhiyaaya vairiyanil kaanapedunna uthppannangal purathuvidunnu. puthiya koshangale baadikkunnathinulla mattu paathakalilekku praveshikkaan kazhiyunna matricks (maa), capsid (caa), nucleocapsid (ensie) enniva eee pilarnna uthppannangalil ulppedunnu.
aarr. ezz. vii cover.
arswick oru glycoprotein adangiya oru cover undu envi. oligomarukalaayi othucherunna glycoproteinukalaaya jipi85, jipi37 enniva chernnathaanu envi. hoost sell resepterumaayi arsv bandhippikkukayum piechu swatantra reethiyil taargett sellumaayi fusion prerippikkukayum cheyyuka ennathaanu enviyude pravartthanam. exocytosis samayat cover labhikkunnu. vairas mukulangal allengil plasma membranilekku thallunnu, ithu hoost sellil ninnu oru puthiya baahya membranumaayi sellil ninnu purathupokaan anuvadikkunnu.
replication saikkil.
sell entry.
virusukalkku aathidheya koshatthilekku praveshikkaan rund vazhikalunda sell risapttar endocytosis allengil fusion. vairas oru resepterine taargett sell membranil bandhippikkukayum vairas koshatthilekku kondupookukayoo endocytos cheiyukayo cheiyunna prakriyayaan endocytosis. endocytosis onnukil piechu swathanthramo piechu aashrithamo aakam. vairas taargett sell membranumaayi samyojippichu athinte genome koshatthilekku purathuvidumbozhaanu fusion sambhavikkunnath. aathidheya kosha stharathinte samyojanathiloode aarr. ezz. vii aathidheya koshatthilekku praveshikkunnu.
transcription.
rsv genome transcription sambhavikkunnathin, oru primer aavasyamaanu. 4s rna ennathu rsv-yude primer 70s rna ennathu dna sinthasisinulla templettumaayi varthikkunnu. aarr. enn. eiii-ashrita dna polymerase aaya reverse traanscriptes, vairal rna poornna neelamulla dna complimentilekku traanskript cheiyunnu.