
gelonin

gelonin ack prakaar 1 ribosome-nishkriya karane vaalaa protein haye aur lagbhag 30 kda kaa vish haye joe himalayan paudhe key gelonium multifluorum key beejon mein paaya jaataa haye. koshika-mukt pranaliyon mein gelonin 4324 site parr adenine koo alag karke eukariotic ribosome kii 28s aar. aar. aine. aeye. ikai parr shaktishali aine-glycosidase gatividhi kartaa haye. gelonin mein carbohydrate-binding domen kaa abhaav hotaa haye isaliye yeh plasma jhilli koo paar karane mein asamarth haye, jisase yeh kewal koshika mukt pranaliyon mein atyadhik prabhaavi haye.
sanrachana.
gelonin 30 kilo dee. aeye. protein haye. gelonin ack diamer haye, jisamen dou samaan monomers hotey hain. pratyek monomer kull 502 avasheshon key liye 251 emino acid seey banaa haye. gelonin koo ack (α + β) protein key roop mein vargeekrit kiya gayaa haye, kyonki isaki maadhyamik sanrachana mein beeta sheet aur alfa helix donon hotey hain. pratyek monomer key pahale 100 amino acid 10 beeta sheet banaate hain, jabaki unake antim 151 amino acid 10 alfa helix banaate hain. geloninuc dwi-mandrak hydrophobic antahkriya aaa hydrogen bandh dwara sthir hoit achhi. vishesh roop seey, anuon koo sthir karane key liye pratyek monomer hydrogen bandhan key aeye. aiss. aine. 22, aar. jee. 178, aeye. aiss. aine. 180 aur ell. aaee. aiss. 237 avashesh ack dusare key saath. isi tarah, ack monomer seey hydrophobic avashesh tyre 14, aaile 15, vail 16 aur pro38 nikatvarti monomer mein samaan avasheshon key saath hydrophobic antahkriya banaate hain taaki diamer koo aur sthir kiya jaa sakey.
sakriya site.
gelonin kaa sakriya sthal chhah pramukh avasheshon dwara banaaya gayaa ack daraar haih tyre74, gly111, tyre113, glue166, aarg169, aur trp198. sakriya sthal kaa aakar gly111 aur tyre113 key beach hydrogen bandhan dwara sthir hotaa haye. tyre113, glue166, aur sakriya sthal mein aarg169 avashesh eukariotic 28aiss aar. aar. aine. key 4324 site parr adenine key enzymatic nishkasana mein bhaag lete hain. haalaanki geloninuc pratikriya tantraken vistaarasan varnit kayalaja rahal achhi, lekin aisaa maanal jaait achhi jey anya prakaar 1 ribosome-nishkriyakaari protein (rip) key beach sanrakshit achhi. anya prakaar 1 rips parr kiye gaye shodh key anusaar, tyre113 aur aarg169 adenine nucleobase mein nitrogen parmaanuon key saath hydrogen bandhan banaate hain. yeh nucleobase aur ribose koo jodane vaale glycoside bandhan key vichhedan koo suvidhajanak banaata haye, jisase sakaratmak roop seey aaveshit oxocarbenium aayan madhyavarti key saath ack sankraman sthiti banati haye. oxocarbenium aayan glue166 key nakaratmak avesh seey sthir hotaa haye. gelonin key sakriya sthal mein teen paani key anu bhee hotey hain, joe nucleophile key roop mein kaary karate hain aur pratikriya koo puuraa karate hue oxocarbenium aayan parr hamla karate hain.
chikitsiya anuprayog.
eukariotic 28s rrna koo alag karke anuvaad koo rokane kii kshamata key kaaran, gelonin mein cancer chikitsa key roop mein upayog karane kii kshamata haye. kaee "inn vitro" model mein gelonin kii cancer virodhi gatividhi kaa pradarshan kiya gayaa haye. haalaanki, isaki hydrophilicity key kaaran, gelonin koshikaon mein aantarik roop seey saksham naheen haye. isase macromolecule key naidaanik anuprayog kathin hoe gaye hain. kaee gelonin vitaran pranaliyon koo engineer kiya gayaa haye, jisamen koshika-pravesh karane vaale peptide key liye sanyugman, listeriolysine oh kaa upayog karke liposome encapsulation aur dwi-vishisht antibody key saath lagaav shamil hain. inn sabhi vitaran pranaliyon koo kaee alag-alag koshika rekhaaon mein tumour key aakar "inn vivo" mein kaafee kamee key liye dikhaaya gayaa haye. haalaanki, geloninuc naidaanik pareekshanken abhee taka adhikrit naheen kiya gayaa haye.