
atiprabhavi dhabba

atiprabhavi daag ack prakaar kaa daag haye jiska upayog sookshmadarshi mein ack jeev seey hataai gaee jeevit koshikaon kii jaanch karane key liye kiya jaataa haye. yeh intravital steining seey alag haye, joe shareer mein daag koo inject karke yaa anyatha pesh karke kiya jaataa haye. iss prakaar ack atee-vishaakt daag mein adhik vishaaktata hoe sakati haye, kyonki kewal kuchh koshikaon koo ise thode samay key liye jeevit rahane kii aavashyakta hoti haye. mahatvapurna daag" shabd kaa upayog kuchh lekhakon dwara vishesh roop seey ack intravital daag koo sandarbhit karane key liye kiya jaataa haye, aur anya dwara ack superavital daag key saath ack dusare key saath, mool avadharana yeh haye kii jaanch kii jaa rahee koshika abhee bhee jeevit haye. choonki koshikaaen jeevit aur binaa chipke hoti hain, shareer key baahar, atiprabhavi daag asthaayi prakriti key hotey hain.
sabse aam atiprabhavi daag reticulocytes parr nae mithileen neele yaa shandar cressil neele rung kaa upayog karke kiya jaataa haye, jisase inn jeevit aparipakva laal rakt koshikaon mein atiprabhavi daagon dwara vishisht roop seey avakshepit ribosome key reticulofilaments pattern koo dekhana sambhav hoe jaataa haye. aisee koshikaon kii sankhya kii ginati karke laal rakt koshika banane kii darr nirdhaarit kii jaa sakati haye, joe asthi majja gatividhi aur anaemia mein ack antardrishti pradaan karati haye. yeh mahatvapurna daag key vipareet haye, jaba prayukt rung vaha hotaa haye jise jeevit koshikaon seey "baahar rakhaa jaataa haye" taaki kewal mrit koshikaaen sakaratmak roop seey dagdar houn. (mahatvapurna daagon mein trippen blue aur propedium iodide jaise rung shamil hain, joe yaa tou bahut bhaari hotey hain yaa koshika jhilli koo paar karane key liye bahut adhik charge hotey hain, yaa joe sakriya roop seey jeevit koshikaon dwara teji seey baahar pump kiye jaate hain.)
agrapakshi dhabbon koo koshika satah antibody dhabbon (immunofluoracence) key saath jodaa jaa sakata haye jaise kii pahalu vishleshan key liye. pratirakshaatmak pratideepti koo jeevit koshikaon key aantarik bhaag mein bhee detergent triton x-100 kaa upayog karke prativarti koshika pargamyata dwara kiya jaa sakata haye. koshikaon kii sankhya key liye uchit sandrata key liye saavdhaanipoorvak samaayojit kiya jaataa haye, poorv upchaar koshika key aantarik bhaag mein 1 aur 150 kilodalton key beach anuon kii pahunch kii anumati dey sakata haye. halanki iss sandarbh mein antibody kaa upayog isi tarah seey kiya jaa sakata haye, lekin "supervital stein" shabd aamtaur parr chhote rasayanon key liye aarakshit haye joe aantarik roop seey upayukt guna rakhate hain.
samanya atiprabhavi rangon key udaaharan.
aar. bee. see. key liye atee-vishisht rangah

