
aiss. bee. pee.-tagg

streptavidine-binding peptide (aiss. bee. pee.)-tagg ack 38-emino acid anukram haye jise punah sanyojak protein mein engineer kiya jaa sakata haye. aiss. bee. pee.-tagg yukt punarsanyoji protein streptavidine seey judate hain aur iss guna kaa upayog vishisht shuddhikaran, pehchaan yaa sthirikaran ranneetiyon mein kiya jaa sakata haye.
aiss. bee. pee. tagg kaa anukram mdekttgwrghvveglageleqlrarrehpqgqrep haye.
khoj.
streptavidine-binding peptide kii khoj aim. aar. aine. aeye. display kii inn vitro chayan takneek kaa upayog karke saat trillion yaadrichhik roop seey utpann peptides key pustakalaya key bheetar kii gaee thee. chayan streptavidine-agrose key saath ushmaayan key baad biotin key saath allution dwara kiya gayaa thaa. aiss. bee. pee.-tagg koo 2.5nm key santulan viyojan sthiraank key saath streptavidine koo baandhate hue dikhaaya gayaa haye aur mool sthitiyon mein biotin key saath aasaani seey baahar nickle jaataa haye.
anuprayog.
protein shuddhikaran.
halke nishkasana kii sthiti (biotin plus wash buffer) key kaaran aiss. bee. pee.-tagg kiye gaye protein koo ack shuddhikaran charan key saath apekshaakrit shuddh sthiti mein utpann kiya jaa sakata haye. kaee apekshaakrit prachur matra mein standhari protein hain joe swabhavik roop seey aaee. aim. aeye. see. matricks key saath judey hotey hain joe adhik samanya roop seey upayog kiye jaane vaale polyhystidine-tagg (unake-tagg) seey judate hain. iss kaaran seey gair-emac shuddhikaran protocol, jisamen aiss. bee. pee.-tagg bhee shamil haye, koo aksar standhari koshikaon mein vyakt kiye jaane vaale protein key liye pasand kiya jaataa haye.
protein jatil shuddhikaran.
antahkriya karane vaale protein key parisaron koo aiss. bee. pee.-tagg kaa upayog karke shuddh kiya jaa sakata haye kyonki biotin key saath nishkasana una sthitiyon mein punarpraapti kii anumati detaa haye jinamen vaanchhit parisar judey rahate hain. udaaharan key liye, condensin parisar koo kima aur anya dwara shuddh kiya gayaa thaa."[2010] aur taaj pratilekhan saha-sakriyak key saath parisaron koo jhaang aur anya dwara shuddh kiya gayaa thaa."[2009]. aiss. bee. pee.-tagg koo kaee tendam affinity purification (tapp) pranaliyon mein bhee shamil kiya gayaa haye jisamen kaee taigon key saath kramik shuddhikaran charanon kaa upayog kiya jaataa haye, udaaharan key liye jee. aff. pee. fusion protein aur bee. tii. key.-protein parisaron koo aiss. bee. pee.-tagg key saath ack tapp protocol kaa upayog karke shuddh kiya gayaa thaa aur unake-tagg, aich. dee. jee. aff.-protein parisaron koo aiss. bee. pee.-tagg key saath ack tapp protocol kaa upayog karke shuddh kiya gayaa thaa aur flagg-tagg aur doubleu. aine. tii. parisaron koo aiss. bee. pee.-tagg aur [shaant-taag] key saath ack tapp protocol kaa upayog karke shuddh kiya gayaa thaa. null kaa upayog aam taur parr protein parisaron key saath kiya jaataa haye aur kaee adhyayan shuddhata aur upaj mein mahatvapurna sudhaar kii report karate hain jaba aiss. bee. pee.-tagg null pranaliyon kii tulanaa gair-aiss. bee. pee.-tagg pranaliyon seey kii jaati haye. aiss. bee. pee.-tagg kaa upayog karane vaali vaanijyik null pranaliyon mein interplay adenoviral aur stanpaayi null pranaaliyan shamil hain joe furtili praudyogikiyon dwara bechi jaati hain, isi tarah key utpaad sigma-aldrich dwara beche jaate hain.
proteomics.
javic roop seey praasangik protein-protein antahkriyaon key liye screen aiss. bee. pee.-tagg aur protein aeye key saath tendam affinity purification (tapp) kaa upayog karke, aiss. bee. pee.-tagg aur protein jee key saath paraspar kriya proteomics aur transcription kaarak parisaron key liye, una proteins key liye joe dengu virus protein denvi-2 ns4aeye key saath aiss. bee. pee.-tagg aur callodulin tagg key saath antahkriya karate hain. aur protein key liye joe protein phosphat 2aeye (pp2aeye) key saath sbp-tagg aur haemaglutinin (haye)-tagg key saath baatchit karate hain.
imaging.
aiss. bee. pee.-tagg bhee ghol mein streptavidine yaa streptavidine abhikarmakon seey juda jaayega. inn engineer sanghon key anuprayogon mein jeevit koshikaon key bheetar vishisht proteins kaa drishya, ack inn vitro anuvaad pranali mein vyaktigat proteins key anuvaad kii gativigyaan kii nigrani, aiss. bee. pee.-tagg koo aine-terminal sthanantaran anukram mein fuse karke endoplasmic reticulum mein ack bahu-faile jhilli protein key ekikaran kaa niyantran aur phir streptavidine key saath ekikaran koo rokna aur biotin key saath ekikaran koo phir seey shuru karna shamil haye. fluorescent streptavidine abhikarmak (ee.jee. streptavidine-aich. aar. pee.) kaa upayog aiss. dee. aiss.-pays key immunoblotting dwara aiss. bee. pee.-tagg kii kalpana karane key liye kiya jaa sakata haye. isake alawa, aiss. bee. pee.-tagg key liye antibody vyaavsaayik roop seey upalabdh hain.
satah plasmon anunaad.
aiss. bee. pee.-tagg kaa upayog streptavidine-karyatmak satahon parr punah sanyojak protein koo prativarti roop seey sthir karane key liye kiya gayaa haye jisase karyatmak satah key punah upayog key saath satah plasmon anunaad (spray) takneekon dwara paraspar kriya mulyankan kii anumati milati haye. aiss. bee. pee.-tagg kii tulanaa anya streptavidine-binding peptides jaise kii strape-tagg key saath karane key liye bhee spray kaa upayog kiya gayaa haye.

