
phosphat-buffer khaara

phosphat-buffer khaara (pee. bee. aiss.) ack buffer samadhan (pee. aich. ~ 7.4) haye jiska upayog aamtaur parr javic anusandhaan mein kiya jaataa haye. yeh ack jall-adharit namak ghol haye jisamen disodium hydrogen phosphat, sodium chloride aur kuchh formulation mein, potasium chloride aur potasium dihydrogen phosphat hotaa haye. buffer ack sthir ph banaae rakhane mein madad kartaa haye. samadhanon kii parasaranta aur aayan sandrata maanav shareer (samasthaanik) seey mell khaati haye.
anuprayog.
pee. bee. aiss. key kaee upayog hain kyonki yeh adhikansh koshikaon key liye samasthaanik aur gair-vishaakt haye. inn upayogon mein padaarth koo kamjor karna aur koshika paatr koo dhona shamil haye. edda key saath pee. bee. aiss. kaa upayog sanlagn aur clumped koshikaon koo alag karane key liye bhee kiya jaataa haye. haalaanki, jastaa jaisi dvivaarshik dhatuon koo jodaa naheen jaa sakata haye kyonki isake parinamswarup varsha hogi. iss prakaar key anuprayogon key liye, achche buffers kii sifarish kii jaati haye. saars-cove-2 jaise aar. aine. aeye. virus key parivahan aur bhandaran key sambandh mein pee. bee. aiss. koo viral parivahan madhyam key liye ack sweekarya vikalp dikhaaya gayaa haye.
taiyari.
pee. bee. aiss. samadhan taiyar karane key kaee alag-alag tareeke hain (unamen seey ack dulbeco kaa phosphat-buffer khaara (dee. pee. bee. aiss.) haye, jisaki sanrachana maanak pee. bee. aiss. kii tulanaa mein alag haye). kuchh formulation mein potasium aur magnicium naheen hotaa haye, jabaki anya mein calcium aur/yaa magnicium hotaa haye (iss baat parr nirbhar kartaa haye kii buffer kaa upayog jeevit yaa nishchit ootak parr kiya jaataa haye yaa naheenh baad vaale koo see. aeye. see. ell. 2 yaa aim. jee. see. ell. 2 kii aavashyakta naheen hoti haye).
sabhi lavanon koo bhang karane key liye 800 millie aasut jall seey shuru karein. kull 1 leater kii matra mein aasut jall jodein. parinami 1 × pbs mein 157 mimi naa +, 140 mimi kla-, 4.45mm key +, 10.1 mimi aich. pee. oh. 42 −, 1.76 mimi aich. pee. oh. 4 − aur 7.96 kaa ack ph. kii antim sandrata hogi. buffer koo 7.3 mimi aich. pee. oh. 42− aur 4.6 mimi aich. pee. oh. 4− key antim ph. key liye 2.4 mimi aur ack see. ell.-sandrata key liye 2.4 mimi mein sthanantarit karane key liye aich. see. ell. kii 2.84 mimi jodein.
pee. bee. aiss. kaa pee. aich. ~ 7.4 haye. jaba buffer samadhan banaate hain, tou hamesha seedhe pee. aich. meater kaa upayog karke pee. aich. koo maapana achchha abhyaas haye. yadi aavashyak hoe, tou hydrochloric acid yaa sodium hydroxide kaa upayog karke ph koo samaayojit kiya jaa sakata haye.
pee. bee. aiss. koo vyaavsaayik roop seey banaai gaee pee. bee. aiss. buffer goliyaan yaa pouch kaa upayog karke bhee taiyar kiya jaa sakata haye.
yadi koshika samvardhan mein upayog kiya jaataa haye, tou samadhan koo alikots mein vitarit kiya jaa sakata haye aur autoclaving yaa nispandan dwara sterilize kiya jaa sakata haye. isake upayog key aadhar parr nasbandi aavashyak naheen hoe sakati haye. pee. bee. aiss. koo kamare key tapman parr yaa refrigerator mein sangraheet kiya jaa sakata haye. haalaanki, kendrit stock samadhan thandaa honey parr avakshepit hoe sakate hain aur upayog seey pahale avakshep poori tarah seey bhang honey taka kamare key tapman parr rakhaa jaanaa chaahiye.