
chromatophoxing

chromatophoxing ack protein-prithakkaran takneek haye joe ekal protein aur anya ampholites koo unake summ-vidyut bindu mein antar key anusaar ack jatil mishran seey samadhan karane kii anumati detee haye. chromatophoxing aayan vinimay regin kaa upayog kartaa haye aur aamtaur parr tez protein taral chromatography (aff. pee. ell. see.) yaa nirantar buffer gradient kaa utpaadan karane mein saksham samaan upkaranon parr kiya jaataa haye, halanki yeh ack aavashyakta naheen haye. 
vishisht aayan vinimay chromatography key vipareet, jahaan buffer vatavaran kii ionic shakti koo badhaakar baadhya anuon koo raal seey baahar nikala jaataa haye, buffer key ph koo badalkar allutes baadhya prajaatiyon koo chromatophocus karate hue. yeh baadhya anuon key shuddh satah avesh koo badal detaa haye, jisase raal key liye unaki pravritti badal jaati haye. jaise hii buffer pranali kaa badalata ph kissy diye gaye anu key paaee koo paar kartaa haye, vaha anu raal seey baahar nickle jaayega kyonki isamen abb shuddh satah charge naheen hoga (aayan vinimay raal key liye aanvik bandhan key liye ack aavashyakta). 
chromatophoxing protein key sambandh mein ack shaktishali shuddhikaran takneek haye kyonki yeh bahut samaan prajaatiyon koo kewal 0.02 pee. aich. ikaaiyon seey alag curr sakati haye joe paaramparik aayan vinimay ranneetiyon kaa upayog karke achchi tarah seey alag naheen hoe sakati hain.
iss takneek kii ack badee kamee yeh haye kii kuchh protein taba ekatrit hotey hain jaba way apekshaakrit uchch sandrata parr maujood hotey hain aur koee shuddh satah charge naheen rakhate hain. yeh raal kii rukawat kaa kaaran bunn sakata haye, joe aff. pee. ell. see. upkaran parr aayan vinimay raal key seelband stambhon kaa upayog karate samay atyadhik samasyagrast hotaa haye, jisake parinamswarup dabaav nirmaan aur sambhaavit upkaran viphalata hoti haye. kabhi-kabhi namoone kii sandrata koo seemit karke aur buffer yojakon key upayog seey spasht ektrikaran key muddon koo door kiya jaa sakata haye joe samagr gathan koo rokte hain.

