
pandinus imperator toxin (pee. aaee. 4)

paaee4 (α-ktx. 6) bichchu "pandinus imperator" seey ack chhota vish haye joe vishisht potasium chainalon koo avaruddh kartaa haye.
vyutpatti.
paaee4 naam bichchu "pandinus imperator" seey alag kiya gayaa chautha vish haye, jisase paaee1, paaee2, paaee3 aur paaee7 koo bhee alag kiya gayaa haye.
rasayan vigyaan.
paaee4 ack peptide haye, jisamen nimnalikhit anukram key saath 38 emino-acid hotey hainh
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isamen ack α-helix aur ack β-sheet hoti haye; yeh chaar cysteine-jodon dwara sthir hoti haye joe chaar chhote diasulphide pulon dwara cross-link hoti hain. chaar diasulphide pull α-ktx6 upa-parivaar key liye vishisht hain, jabaki adhikansh anya bichchu vishaakt padarthon mein kewal teen diasulphide pull hotey hain. paaee4 key cysteine koo nimnalikhit crum mein jodaa jaataa haih 6see-27see; 12see-32see; 16see-34see; 22see-37see.
adhikansh diasulphide pull baaen haath seey sanrachana dikhaate hain, halanki 22see-37see key beach diasulphide pull mein kuchh bhinnata paaee jaati haye. kewal 6see-27see key beach kaa diasulphide pull daahine haath kii sanrachana dikhaata haye.
lakshya aur kaarravaai kaa tarika.
paaee4 vibhinn potasium chainalon koo avaruddh kartaa haye, udaaharan key liye, shekar bee, kv1.2, aur eske potasium channel.
"shekar" bee channel.
paaee4 shekar bee potasium chainalon seey judataa haye, voltage-gated potasium channel kv1.1 kaa drosophila homolog. paaee4 iss channel koo 3 ± 2.2 nm key ic50 key saath prativarti roop seey avaruddh kartaa haye.
ack paaee4 peptide, praakritik paaee4 (koech2aine key bajaay coao-) kii tulanaa mein ack alag see-terminus key saath sanshleshit, praakritik paaee4 key samaan bandhan visheshtaon koo darshaata haye. isase pataa chalata haye kii peptide paaee4 kaa see-terminus shekar bee channel key liye paaee4 key bandhan mein shamil naheen haye.
avashesh 26k, 28i, 29n, 33k, aur 35y shekar bee chainalon key saath paaee4 kii baatchit key liye mahatvapurna hoe sakate hain, vishesh roop seey 26k aur 35y, joe anya potasium channel vishaakt padarthon mein ack lycine aur sugandhit samooh kaa ack sanrakshit rung banaate hain. yeh sujhaav diyaa gayaa haye kii lycine kaa sakaratmak avesh ack potasium aayan kii nakal kartaa haye aur potasium channel key chhidra mein pravesh kartaa haye, isaliye chhidra key udghaatan koo rokta haye aur aayan pravaah koo rokta haye.
kv1.2 channel.
pee. aaee. 4 voltage-gated potasium channel koo cumm sandrata (aaee. see. 50 8 ± 5 pee. aim.) parr avaruddh kartaa haye. 10 microne taka kii sandrata parr kv1.1 aur kv1.3 chainalon parr koee mahatvapurna prabhaav naheen dekhaa gayaa haye.
peptide paaee4 koo kv1.2 channel seey jodane mein, β-sheet sanrachana koo ack mahatvapurna bhoomika nibhaane key liye socha jaataa haye. sabse pahale, β-sheet sanrachana mein sthit avashesh 35y, kv1.2 chainalon (344w, 345w aur 355y) key sugandhit samooh key saath sthir vidyut balon key madhyam seey kaskar baatchit kartaa haye. doosara, avashesh 26k chaar kv1.2 α-upghatakon (357d) dwara banaae gaye chhidra key channel mein sthit chaar carbonyl oxygen parmaanuon dwara sthir hoe jaataa haye. anta mein, kv1.2 chainalon key chaar α-upa-ikaaiyon key chaar 332q avashesh vish ring (10r, 19r, 30k, 33k seey baney) key chaar upa-ikaaiyon key saath namak pulon key madhyam seey baatchit karate hain.
aiss. key. channel.
isake alawa, paaee4 aiss. key. chainalon, chhote chaalan see. aeye. 2 +-sakriya potasium chainalon seey judataa haye. paaee4 apamine, ack anya aiss. key. channel toxin key saath pratispardha kartaa haye. aaee. see. 50 0.5 ± 0.2 microne haye.
vishaaktata.
bichchu kaa vish standhaariyon, keedon aur crustatian key liye vishaakt hoe sakata haye. mastishk key nilay pranali mein injection lagaane parr choohe mein paaee4 ghaatak hotaa haye aur ell. dee. 50 maan 0.2 microgram/mouse hotaa haye.