pratijan punarpraapti

ootak joe formaldehyde key saath sanrakshit kiye gaye hain, ack atyadhik pratikriyaasheel yaugik, vibhinn prakaar key rasayanik sanshodhan hotey hain joe pratiraksha-rasayan jaisi jaiv chikitsa prakriyaon mein protein kii pehchaan koo cumm curr sakate hain. pratijan punarpraapti inn rasayanik sanshodhanon koo cumm karane yaa samaapt karane kaa ack tarika haye. pratijan punarpraapti key dou praathamik tareeke garmi-madhyasth epitope punarpraapti (hier) aur proteolytic prerit epitope punarpraapti (pier) hain.
protease-prerit epitope punarpraapti (ghaat).
pier mein, protein key, tripsin aur pepsin sahit enzymon kaa upayog antibody koo isake epitope mein baandhane key liye kiya gayaa haye. kriya kaa kathit tantra epitope koo chhipaate hue peptides kaa daraar haye, joe pratijanta koo bahaal kartaa haye.
ghaat key nuksan mein pratirakshaatmaktaa koo bahaal karane key liye cumm safalata darr, aur ootak aakriti vigyaan aur ruchi key pratijan kaa sambhaavit vinash shamil haye.
ooshmaa-prerit epitope punarpraapti (hier).
maanaa jaataa haye kii hier kuchh cross-link koo ulat detaa haye, aur epitope kii tritiyak sanrachana key dwiteeyak koo bahaal karane kii anumati detaa haye. protocol key liye pratyek ootak, nirdhaaran vidhi aur adhyayan kiye jaane vaale pratijan key liye anukoolan kii aavashyakta hoti haye.
samanya taur parr, hier kii safalata darr ghaat kii tulanaa mein bahut adhik haye. hier koo microwave ovan, pressure cooker, sabji steamer, autoclave yaa paani key snan key saath kiya jaa sakata haye. samay, tapman, buffer aur ph-samvedansheel haye.
kamare key tapman epitope punarpraapti (aar. tii. aar.).
aar. tii. aar. mein hydrochloric acid (pee. aich. 1) yaa formic acid (pee. aich. 2) jaise acid kaa upayog karke cross-link kaa ulatana shamil haye.
jamey hue khand epitope punarpraapti (aff. aiss. aar.).
aff. aiss. aar. dou pratijan punarpraapti vidhiyon koo sandarbhit kartaa haye jinhen vishesh roop seey aldehyde-fixed cryostate jamey hue ootak vargon yaa samvardhit koshikaon key liye design kiya gayaa haye; aiss. dee. aiss. aur heating aine block vidhiyaan.
aiss. dee. aiss. vidhi key liye 1 pratishat aiss. dee. aiss. kaa upayog karke 5 minut key sankshipt upchaar kii aavashyakta hoti haye aur yeh aaee. aich. see. key saath-saath immunofluorescence dwara dhundhlapan kii teevrata mein vriddhi curr sakata haye.
heating aine block paraphormaldehyde mein sthir ootak block letaa haye, unhein punarpraapti samadhan mein germ kartaa haye, aur phir sookhi burf kaa upayog karke unhein freeze curr detaa haye. heating kaa upayog pahale seey hii aeye. aar. mein kiya jaataa haye aur vyaapak roop seey prabhaavi saabit huaa haye, lekin pichhale heating vidhiyon koo jamey hue vargon koo maarne key liye dikhaaya gayaa haye. yeh vidhi antigen kii ack vistrit shrinkhala key liye pratirakshaatmaktaa koo badhaane aur kuchh mamalon mein prishthabhoomi key daag koo cumm karane key liye saabit hui.

