
matricks-sahaayata praapt aayaneekaran

maas spectrometry mein, matricks-sahaayata praapt aayaneekaran (inlet aayaneekaran bhee) ack cumm vikhandan (naram) aayaneekaran takneek haye jisamen vayumandaliya dabaav (appy) seey vishleshan aur matricks namoone key kanon koo germ inlet tube mein sthanantarit karna shamil haye joe appy kshetra koo maas vishleshak key nirvaat seey jodata haye.
praarambhik aayaneekaran taba hotaa haye jaba inlet tube key bheetar dabaav cumm hoe jaataa haye.
inlet aayaneekaran electrospre aayaneekaran key samaan haye jisamen ack vipareet charan vilaayak pranali kaa upayog kiya jaataa haye aur utpaadit aayan atyadhik aaveshit hotey hain, halanki hamesha voltage yaa lazer kii aavashyakta naheen hoti haye. yeh peptides, protein aur lipids jaise chhote aur badey anuon key liye ack atyadhik samvedansheel prakriya haye jise taral chromatograph seey jodaa jaa sakata haye.
inlet aayaneekaran takneekon kaa upayog orbitrap maas analyzer, orbitrap forier transform maas spectrometer, raikhik trap quadrupol aur maldi-toff key saath kiya jaa sakata haye.
inlet aayaneekaran key prakaar.
matricks-sahaayata praapt inlet aayaneekaran.
matricks-sahaayata praapt inlet aayaneekaran (maaee) mein, ack matricks joe ack vilaayak hoe sakata haye, kaa upayog parivesh key tapman parr ack mishran key roop mein ruchi key vishleshan key saath kiya jaataa haye. matricks/analyte mishran koo tube key shuruati chhor parr mishran koo tapp karke germ inlet tube mein daala jaataa haye. aayaneekaran seey utpann honey vaale analyte key atyadhik aaveshit ayanon key liye, matricks anuon kaa vighatan honah aavashyak haye.
jinn matricks kaa upayog kiya jaa sakata haye unamen shamil hainh
2, 5-dihydroxybenzoic acid, 
2, 5-dihydroxiacetophene, 
2-eminobangeal alcohol, 
anthronylic acid, aur 
2-hydroxyacetophenone.
lazer spray inlet aayaneekaran.
laserprey inlet aayaneekaran (ell. aiss. aaee. aaee.) maaee kaa ack upasamuchchay haye aur ack matricks-sahaayata praapt lazer avashoshan/aayaneekaran (maldi) vidhi kaa upayog kartaa haye. ise mool roop seey vayumandaliya dabaav matricks-sahaayata praapt lazer apashoshan/aayaneekaran kahaa jaataa thaa, lekin maldi key saath bhram seey bachane key liye iska naam badalkar ell. aiss. aaee. aaee. curr diyaa gayaa thaa aur kyonki yeh ack prakaar kaa inlet aayaneekaran paaya gayaa thaa. sabhi inlet aayaneekaran takneekon key roop mein, atyadhik gunaa aaveshit ayanon kaa utpaadan hotaa haye. ack nitrogen lazer kaa upayog germ inlet tube mein thhos matricks/analyte koo ghataane key liye kiya jaataa haye, avalokan kiye gaye aayan matricks/analyte kii satah parr utpann hotey hain aur isaliye lazer seedhe aayaneekaran mein shamil naheen hotaa haye jaisa kii mool roop seey socha gayaa thaa. ell. aiss. aaee. aaee. protein aanvik bhaar nirdhaarit curr sakata haye aur 20,000 dee. aeye. taka protein key dravyamaan kaa pataa lagaane key liye paaya gayaa haye. protein kaa pataa lagaane key liye ell. aiss. aaee. aaee. kii samvedansheelta, ee. aiss. aaee. kii tulanaa mein parimaan key crum seey adhik hoti haye.
vilaayak sahaayata praapt inlet aayaneekaran.
vilaayak sahaayata praapt inlet aayaneekaran (saaee) matricks-sahaayata praapt inlet aayaneekaran key samaan haye, halanki matricks paani, acetonitrile aur methanol jaise vilaayak hain. yeh aayaneekaran takneek chhote anuon, peptides aur protein key prati atyadhik samvedansheel haye.
analyte koo vilaayak mein bhang curr diyaa jaataa haye aur yaa tou ack keshika stambh dwara germ inlet tube mein pesh kiya jaa sakata haye yaa seedhe ack syringe key saath inlet tube mein yaa pipeting dwara inject kiya jaa sakata haye. keshika stambh fused silika kanon seey banaa hotaa haye jiska ack chhor namuna vilaayak mein duba hotaa haye aur doosara germ inlet tube key anta mein hotaa haye. pariveshiya dabaav aur nirvaat key beach dabaav antar key kaaran vilaayak pump key upayog key binaa keshika stambh key madhyam seey bahata haye.
inlet tube mein tapman 50 degree celsius seey 450 degree celsius taka bhinn hoe sakata haye, cumm tapman kaa upayog kiya jaa rhaa haye yadi uchch tapman seey praapt parinam achche resolution key hain. vilaayak sahaayata praapt inlet aayaneekaran koo naa kewal taral chromatography (ell. see.) key saath jodaa jaa sakata haye, balki naino ell. see. key saath bhee jodaa jaa sakata haye.
inlet aayaneekaran key laabh.
vayumandaliya dabaav parr aayaneekaran aksar pariveshiya dabaav kshetra seey dravyamaan vishleshak key nirvaat mein ayanon key hastaantaran key dauran ayanon key nuksan kaa kaaran banata haye. aayan vishleshan spray key failaav aur 'rima loss' key kaaran khou jaate hain, jisase cumm aayan m/z prithakkaran key liye nirvaat taka pahunchate hain. praarambhik aayaneekaran germ inlet tube key upa-vayumandaliya dabaav kshetra mein hotaa haye joe seedhe dravyamaan vishleshak key nirvaat seey judaa hotaa haye aur isaliye ayanon kaa hastaantaran naheen honey parr aayan kaa nuksan cumm hoe jaataa haye.
ell. aiss. aaee. aaee. mein lazer kaa upayog behtar sthaanik resolution kaa utpaadan karke parinamon kii chhavi gunavatta koo badhaata haye. yahee vaha jagah haye jahan adhik pixel banaae jaate hain aur isaliye ack spasht chhavi praapt kii jaati haye.
gunak aaveshit ayanon kaa utpaadan hotaa haye 
dravyamaan seema koo aur vistaarit karna.
sanrachanaatmak jaankaari key liye vikhandan kaa utpaadan karane vaale anuon koo vikhandit karane key liye kaee tarikon kaa upayog kiya jaa sakata haih
electron hastaantaran prithakkaran (ee. tii. dee.), 
takraav-prerit vighatan (see. aaee. dee.), aur 
electron grahan prithakkaran (ee. see. dee.).
lazer kaa upayog karate samay, kewal chhoti matra kii aavashyakta hoti haye.

