text:
How do we know that Paul knows what he’s talking about? Why can he bear witness? Is it just because he is a Jew himself?
It is not only because he is a Jew, but rather that he is “a Pharisee and the son of a Pharisee,” as he describes himself in Acts 23:6. In other words, Paul is himself an expert in Jewish law, Jewish religious life and Jewish spirituality, and raised by one, too. He’s been around and knows what he’s talking about. And what is more, Paul still expresses his love for his fellow Jews, even though they have now become his enemies.
So when Paul says that he knows the Jews and that they have a “zeal for God, but not according to knowledge,” we have good reason to believe him. He’s experienced their life, learned from their great teachers and even become one of their experts in Jewish life.
The historical meaning we can read here is that Paul is pointing to the way the Jews of his time observed the Law of Moses. The Pharisees especially would follow it as literally and as exactly as possible, even if it meant the neglect of those around them who needed their help.
We saw many times, for instance, how the Pharisees criticized Jesus for supposedly violating the Sabbath requirement not to work when He healed someone. What mattered to them was the rules, not the suffering of a human person.
How could that have been fixed? The key is in the word knowledge. Man’s purpose is to know God, and knowing God is even defined by Christ Himself as what salvation actually is (John 17:6). And Jesus says that He knows the Father and the Father knows Him (John 10:15). Thus, the “knowledge” according to which proper zeal operates is a knowledge of God, of knowing God and being known by Him. It is love. It is union and communion.
Thus, if the Pharisees had known God, had truly loved God, then they would have seen the purpose of the Law was not to weigh people down with heavy burdens of exactness but rather to lift them up to loving communion with the Father. And had the Jews known God, they would have received Him when He came in the flesh. Yet they crucified Him.
Sadly, though, lack of this kind of knowledge of God persists. We can see the Pharisees’ ignorant zeal sometimes in church life, when people become so obsessed with the canons and other rules of the Church that they neglect the suffering of those at hand, perhaps even causing suffering by demanding that such rules be followed, rules that while important are not dogmatic or moral teachings.
An example might be breaking communion with fellow Orthodox Christians because they do not follow the same liturgical calendar. Another might be demanding that people fast or do piety in a certain way and then condemning or mistreating them if they do not.
Such rules and traditions are of course important and ultimately indispensable in their context, but if they aren’t being used to bring people to Jesus Christ, they are being misused. That does not mean that we can violate the dogmas or moral teachings of the Church, but it does mean that other kinds of Church traditions have to be applied with good sense and most especially love.
We can speak of another kind of “zeal for God” that is “not according to knowledge.” Often, in church life, we can “major in the minors” in other ways. Some people have a zeal for many elements in church life that may in themselves be helpful or at least harmless but can also be used to distract from the “one thing needful” (Luke 10:42).
For instance, someone might give money to church. Or he might participate in many educational classes and events. Or he might work in the kitchen or at coffee hour. Or he might sing in the choir or at the chanter’s stand. Or he might be teaching. Or he might go to many social events. Or he might donate piles of goods for the needy. Or pick almost anything else. He might love whatever it is and really throw himself into it. He might be the very best at it.
But if he is not engaged in the spiritual life of struggle against sin, of genuine prayer both corporately and privately, of participation in the sacraments, of love for his fellow Christians and most especially love for God, then all this has become a zeal without knowledge. It is possible to be very near to God all the time and yet be very far away from Him at the same time. Again, all these things are good, but if we do not come to know God Himself better through them, then they are useless and even harmful to us.
Finally, there is another kind of zeal that is not according to knowledge that we can see in our time. Some people become obsessed with all the details of Orthodox theology or praxis yet do not know Jesus Christ in His personal warmth and love. They love the religion but not the One Whom the religion points to. They love the spirituality but not the One Who sends the Holy Spirit.
This kind of ignorant zeal is a temptation especially for the clergy and for the new convert, though it can afflict anyone. This zeal creates a religion that may be glorious and stunning but ultimately cold and demanding. It may be expressed as rigorism or ritualism or esotericism or false traditionalism. In all cases, what is true and good in the Orthodox Christian faith is turned into an identity marker for correctness rather than a salvific path toward communion with God.
If we are to have a zeal for God that is indeed according to knowledge, we should focus first on that knowledge and then let the zeal come of its own accord. Does not a man who defends his beloved wife from danger have a zeal that comes from his knowledge of her? Does not a mother who watches over her children with care and energy have a zeal that comes from her knowledge of them? Do not friends who dedicate all they have to staying connected with one another have a zeal that comes from their knowledge of each other?
Do you see how zeal is the result of love? It is not a substitute for love. Enthusiasm and seriousness are not the same thing as love. If we are to have a true zeal for God, then we must gain it through knowledge of Him.
And how do we gain that knowledge of Him? Our faith comes by hearing the word of God (Rom. 10:17), by prayer, by active love for His children, by participation in the sacraments, by earnestly seeking to know Him in every way. When we do that, the zeal will come of its own accord. Our energy for doing God’s work will come from knowing God’s heart.
To our Lord Jesus Christ therefore be all glory, honor and worship, with His Father and the Holy Spirit, now and ever, and unto ages of ages. Amen.
I have relied here significantly on the commentary of the late Archbishop Dmitri (Royster) of Dallas, in his work on Romans 10 as well as Romans 8. The edition of his commentary I’m using is a draft edition from 2004-05.
thank you Father Damick, I have always believed this, you describe what zeal for Orthodoxy is really about, perfectly.

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How do we know that Paul knows what he ’s talking about ? Why can he bear witness ? Is it just because he is a Jew himself ?
 It is not only because he is a Jew , but rather that he is “ a Pharisee and the son of a Pharisee , ” as he describes himself in Acts 23:6 . In other words , Paul is himself an expert in Jewish law , Jewish religious life and Jewish spirituality , and raised by one , too . He ’s been around and knows what he ’s talking about . And what is more , Paul still expresses his love for his fellow Jews , even though they have now become his enemies .
 So when Paul says that he knows the Jews and that they have a “ zeal for God , but not according to knowledge , ” we have good reason to believe him . He ’s experienced their life , learned from their great teachers and even become one of their experts in Jewish life .
 The historical meaning we can read here is that Paul is pointing to the way the Jews of his time observed the Law of Moses . The Pharisees especially would follow it as literally and as exactly as possible , even if it meant the neglect of those around them who needed their help .
 We saw many times , for instance , how the Pharisees criticized Jesus for supposedly violating the Sabbath requirement not to work when He healed someone . What mattered to them was the rules , not the suffering of a human person .
 How could that have been fixed ? The key is in the word knowledge . Man ’s purpose is to know God , and knowing God is even defined by Christ Himself as what salvation actually is ( John 17:6 ) . And Jesus says that He knows the Father and the Father knows Him ( John 10:15 ) . Thus , the “ knowledge ” according to which proper zeal operates is a knowledge of God , of knowing God and being known by Him . It is love . It is union and communion .
 Thus , if the Pharisees had known God , had truly loved God , then they would have seen the purpose of the Law was not to weigh people down with heavy burdens of exactness but rather to lift them up to loving communion with the Father . And had the Jews known God , they would have received Him when He came in the flesh . Yet they crucified Him .
 Sadly , though , lack of this kind of knowledge of God persists . We can see the Pharisees ’ ignorant zeal sometimes in church life , when people become so obsessed with the canons and other rules of the Church that they neglect the suffering of those at hand , perhaps even causing suffering by demanding that such rules be followed , rules that while important are not dogmatic or moral teachings .
 An example might be breaking communion with fellow Orthodox Christians because they do not follow the same liturgical calendar . Another might be demanding that people fast or do piety in a certain way and then condemning or mistreating them if they do not .
 Such rules and traditions are of course important and ultimately indispensable in their context , but if they are n’t being used to bring people to Jesus Christ , they are being misused . That does not mean that we can violate the dogmas or moral teachings of the Church , but it does mean that other kinds of Church traditions have to be applied with good sense and most especially love .
 We can speak of another kind of “ zeal for God ” that is “ not according to knowledge . ” Often , in church life , we can “ major in the minors ” in other ways . Some people have a zeal for many elements in church life that may in themselves be helpful or at least harmless but can also be used to distract from the “ one thing needful ” ( Luke 10:42 ) .
 For instance , someone might give money to church . Or he might participate in many educational classes and events . Or he might work in the kitchen or at coffee hour . Or he might sing in the choir or at the chanter ’s stand . Or he might be teaching . Or he might go to many social events . Or he might donate piles of goods for the needy . Or pick almost anything else . He might love whatever it is and really throw himself into it . He might be the very best at it .
 But if he is not engaged in the spiritual life of struggle against sin , of genuine prayer both corporately and privately , of participation in the sacraments , of love for his fellow Christians and most especially love for God , then all this has become a zeal without knowledge . It is possible to be very near to God all the time and yet be very far away from Him at the same time . Again , all these things are good , but if we do not come to know God Himself better through them , then they are useless and even harmful to us .
 Finally , there is another kind of zeal that is not according to knowledge that we can see in our time . Some people become obsessed with all the details of Orthodox theology or praxis yet do not know Jesus Christ in His personal warmth and love . They love the religion but not the One Whom the religion points to . They love the spirituality but not the One Who sends the Holy Spirit .
 This kind of ignorant zeal is a temptation especially for the clergy and for the new convert , though it can afflict anyone . This zeal creates a religion that may be glorious and stunning but ultimately cold and demanding . It may be expressed as rigorism or ritualism or esotericism or false traditionalism . In all cases , what is true and good in the Orthodox Christian faith is turned into an identity marker for correctness rather than a salvific path toward communion with God .
 If we are to have a zeal for God that is indeed according to knowledge , we should focus first on that knowledge and then let the zeal come of its own accord . Does not a man who defends his beloved wife from danger have a zeal that comes from his knowledge of her ? Does not a mother who watches over her children with care and energy have a zeal that comes from her knowledge of them ? Do not friends who dedicate all they have to staying connected with one another have a zeal that comes from their knowledge of each other ?
 Do you see how zeal is the result of love ? It is not a substitute for love . Enthusiasm and seriousness are not the same thing as love . If we are to have a true zeal for God , then we must gain it through knowledge of Him .
 And how do we gain that knowledge of Him ? Our faith comes by hearing the word of God ( Rom . 10:17 ) , by prayer , by active love for His children , by participation in the sacraments , by earnestly seeking to know Him in every way . When we do that , the zeal will come of its own accord . Our energy for doing God ’s work will come from knowing God ’s heart .
 To our Lord Jesus Christ therefore be all glory , honor and worship , with His Father and the Holy Spirit , now and ever , and unto ages of ages . Amen .
 I have relied here significantly on the commentary of the late Archbishop Dmitri ( Royster ) of Dallas , in his work on Romans 10 as well as Romans 8 . The edition of his commentary I ’m using is a draft edition from 2004 - 05 .
 thank you Father Damick , I have always believed this , you describe what zeal for Orthodoxy is really about , perfectly .


text:
Note: The Unity Uploader is a community project run by our awesome friend, Anthony Madden, with support from us. If you're interested, please join the Slack and join the #unity-uploader.
The Uploader allows you to use a Unity scenes as Template for your Worlds. You can bring in a haunted house for Halloween or your favorite creation from Minecraft. If you can import it into Unity, you can probably get it into Altspace this way. Here are a few example Worlds.
Join the SDK Slack - Friends don't let friends build Worlds alone.
Install Unity Hub and install Unity 2018.1.9f2. The Uploader will not work unless you match this version exactly. You'll need a free Unity account if you don't have one and choose "Personal" since you're doing this for fun! During the install, make sure you check the "Android Builds" option and disable auto-update.
Create a Template on our website. Name it "Hello World Template".
Create a World and name it "Hello World". Select "Hello World Template" as the Template.
Open Unity Hub and create a new Unity 2018.1.9f2 project.
Click "Load Templates" and then select "Hello World Template"
Add a cube to your scene and save.
Check "Build for Windows?" and uncheck "Build for Android?"
Click "Upload". In about a minute you should see "Upload" complete.
Yes: models, collision, animations, particle effects, audio, skyboxes, etc.
On the Template page on the left side, we show you the latest uploads by platform. If it was successful you'd see "1-2mins ago".
You can be in-World when you update. The moment the Uploader says "Upload Complete" you can reset the World to see the changes.
Did you save the scene in Unity?
Did you choose the platform you're testing on?
Are you in the right World? Did you choose the right Template in the Uploader AND in the World form?
Did you check the Template page stats?
Most common upload error is having the wrong Unity version. It must match the required version exactly.
Your upload might be too big. Try to keep PC scenes < 100MB. Start small and build up. Optimize, optimize, optimize.
How do I add custom spawn points to my Worlds?

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Note : The Unity Uploader is a community project run by our awesome friend , Anthony Madden , with support from us . If you 're interested , please join the Slack and join the # unity - uploader .
 The Uploader allows you to use a Unity scenes as Template for your Worlds . You can bring in a haunted house for Halloween or your favorite creation from Minecraft . If you can import it into Unity , you can probably get it into Altspace this way . Here are a few example Worlds .
 Join the SDK Slack - Friends do n't let friends build Worlds alone .
 Install Unity Hub and install Unity 2018.1.9f2 . The Uploader will not work unless you match this version exactly . You 'll need a free Unity account if you do n't have one and choose " Personal " since you 're doing this for fun ! During the install , make sure you check the " Android Builds " option and disable auto - update .
 Create a Template on our website . Name it " Hello World Template " .
 Create a World and name it " Hello World " . Select " Hello World Template " as the Template .
 Open Unity Hub and create a new Unity 2018.1.9f2 project .
 Click " Load Templates " and then select " Hello World Template "
 Add a cube to your scene and save .
 Check " Build for Windows ? " and uncheck " Build for Android ? "
 Click " Upload " . In about a minute you should see " Upload " complete .
 Yes : models , collision , animations , particle effects , audio , skyboxes , etc .
 On the Template page on the left side , we show you the latest uploads by platform . If it was successful you 'd see " 1 - 2mins ago " .
 You can be in - World when you update . The moment the Uploader says " Upload Complete " you can reset the World to see the changes .
 Did you save the scene in Unity ?
 Did you choose the platform you 're testing on ?
 Are you in the right World ? Did you choose the right Template in the Uploader AND in the World form ?
 Did you check the Template page stats ?
 Most common upload error is having the wrong Unity version . It must match the required version exactly .
 Your upload might be too big . Try to keep PC scenes < 100 MB . Start small and build up . Optimize , optimize , optimize .
 How do I add custom spawn points to my Worlds ?


text:
Complete range of fuel dispensers for non-commercial use. Sturdy structure fitted with front door for easy maintenance operations. High capacity vane pump. Inbuilt bypass. Automatic nozzle with swivel connection. Inbuilt water absorption filter (or on request according to model) to ensure that dispensed product is free of impurities.
Fitted with oval gear meters and an electronic unit for multiuser control and pre-selected dispensing. Local memory capable of storing data for last 255 operations. Interfaces with PC for sending and organizing data. Possibility of entering vehicle registration number, kilometers, date and time of delivery. Magnetic keys (Kit keys) available for user identification.
On request: a dedicated software for printing individual summaries of dispensing details.
Users : no. 120 max.

tokenized:
Complete range of fuel dispensers for non - commercial use . Sturdy structure fitted with front door for easy maintenance operations . High capacity vane pump . Inbuilt bypass . Automatic nozzle with swivel connection . Inbuilt water absorption filter ( or on request according to model ) to ensure that dispensed product is free of impurities .
 Fitted with oval gear meters and an electronic unit for multiuser control and pre - selected dispensing . Local memory capable of storing data for last 255 operations . Interfaces with PC for sending and organizing data . Possibility of entering vehicle registration number , kilometers , date and time of delivery . Magnetic keys ( Kit keys ) available for user identification .
 On request : a dedicated software for printing individual summaries of dispensing details .
 Users : no . 120 max .


text:
The chaos that the shortage of qualified Primary-Care Physicians is causing in the United States is glaringly obvious. It does not help that more and more family doctors announce they are giving up their practice in favor of becoming private doctors, taking cash-only patients while others decide to sell their practices to hospitals.
In 2012, the New York Times reported that America was short almost 9,000 Primary-Care Physicians. That number is expected to have gone up drastically since then as more are being let go or choosing to let go voluntarily. This shortage is expected to grow even more acute in the next 15 years, with a projected shortfall of an estimated 65,000 doctors. These are family doctors, general internists, general pediatricians and geriatricians – the doctors who diagnose new illnesses, manage chronic ones, advocate preventive care and protect wellness.
Take a look at some of the most compelling reasons why choosing to become a primary care physician in the US today is an excellent idea.
According to the American Academy of Family Physicians, the demand for family physicians in the USA is expected to exceed the supply by the year 2020. In such a scenario, the demand for primary-care physicians is expected to grow exponentially as the shortage worsens. This means you are never going to be out of a job for a long time to come.
For a long time, one of the main reasons why this specialty was often featured last on the list of most preferred fields for med students was the low wages associated with it. A lot has changed in recent years though. According to a report published by a reputed medical magazine lately, the average earnings for primary-care physicians shot up by about 5.2%, which is a faster rise than those of other specialists.
Moreover, primary-care physicians working in underserved regions enjoy handsome tax benefit incentives with Medicare currently providing a 10 % bonus payment.
In the past, many people would only go to hospital emergency rooms when they got sick instead of seeing a regular doctor. All of this is expected to change as of January 1, 2014 when the Obamacare insurance plans or the Affordable Care Act comes into effect and the newly insured start seeking care. Many authorities project that the growing patient demand for services could cause the health care system to crash. The newly insured will all need Primary-Care Providers.
The practice of primary care medicine is not easy. In fact, it is often characterized as one of the most challenging careers within the field of medicine. Despite this, the immense personal satisfaction and pleasure that is derived from successful patient care is what often drives future PCPs into the field.

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The chaos that the shortage of qualified Primary - Care Physicians is causing in the United States is glaringly obvious . It does not help that more and more family doctors announce they are giving up their practice in favor of becoming private doctors , taking cash - only patients while others decide to sell their practices to hospitals .
 In 2012 , the New York Times reported that America was short almost 9,000 Primary - Care Physicians . That number is expected to have gone up drastically since then as more are being let go or choosing to let go voluntarily . This shortage is expected to grow even more acute in the next 15 years , with a projected shortfall of an estimated 65,000 doctors . These are family doctors , general internists , general pediatricians and geriatricians – the doctors who diagnose new illnesses , manage chronic ones , advocate preventive care and protect wellness .
 Take a look at some of the most compelling reasons why choosing to become a primary care physician in the US today is an excellent idea .
 According to the American Academy of Family Physicians , the demand for family physicians in the USA is expected to exceed the supply by the year 2020 . In such a scenario , the demand for primary - care physicians is expected to grow exponentially as the shortage worsens . This means you are never going to be out of a job for a long time to come .
 For a long time , one of the main reasons why this specialty was often featured last on the list of most preferred fields for med students was the low wages associated with it . A lot has changed in recent years though . According to a report published by a reputed medical magazine lately , the average earnings for primary - care physicians shot up by about 5.2 % , which is a faster rise than those of other specialists .
 Moreover , primary - care physicians working in underserved regions enjoy handsome tax benefit incentives with Medicare currently providing a 10 % bonus payment .
 In the past , many people would only go to hospital emergency rooms when they got sick instead of seeing a regular doctor . All of this is expected to change as of January 1 , 2014 when the Obamacare insurance plans or the Affordable Care Act comes into effect and the newly insured start seeking care . Many authorities project that the growing patient demand for services could cause the health care system to crash . The newly insured will all need Primary - Care Providers .
 The practice of primary care medicine is not easy . In fact , it is often characterized as one of the most challenging careers within the field of medicine . Despite this , the immense personal satisfaction and pleasure that is derived from successful patient care is what often drives future PCPs into the field .


text:
What is a ready dating website by Dating Pro Professional dating site in 1 day You pick a package, we do the rest. Full technical documentation from developers for developers. Open to modification by any PHP developer. No “powered-by,” no ads unless you want them for making money.
He wanted to make plans to hang out some time. A specific place and time.
Paper World offers offers original newspapers, magazines and other memorabilia from the day you were born. Unique gift ideas for a birthday present, Mother’s Day gift and more birthday gift ideas.
See some words or phrases that you don’t understand? Check out The Dragon’s Lexicon. My dreams consist of love, laughter, and living life to its fullest. A lover of fine wine and food, I tend to break the bank of those who can handle me.
He might answer a few emails. Or possibly take a quick call. I have to get going. Often, I stick around without him. What kind of madman voluntarily works on a Friday night? All of those words sound funny. Like they should be used to describe someone much older. But weirdly, they apply to Rand. And have for years. We go on the road together. The answer really should be no. And yet, when they ask, all I can say is yes. I miss him constantly.
Always tugging at those damn heartstrings of mine when he leaves a room.
Check out our members now! Join free, anonymous and instantly! Meet new people and start dating! You can send messages anonymously to anyone you like. Start meeting new people today and discover the real potential of this website. You have probably been waiting for a chance like this – now start to discover how enjoyable life can really be!
Single marriage-minded women only. We offer 4, easy to understand, services with one downpayment of Euro for all 4. We will introduce you to ALL girls from Ukraine in our database meeting your criteria. You will get a list of those who interested and direct contact of 2 ladies wanting you to write them! Direct contact info of ONE girl: This means she is opened for relationship with you and expecting your contact. You may select several girls to see who likes you and get in touch with just one.
It was the last time she saw him. I have countless emails from readers telling me stories of guys and women who moved the initial dating period along at high speed.
Hello I’ve been in a relationship with a widower for a little over a year. When I met him, it was 3 years since he’d lost his wife. I was the first girlfriend he’d had in that time.
Organizing trip for girl you want to meet in your or third country: Introduction tour to Kiev to meet ALL girls you like: See who has Upcoming Birthday – write a special Birthday letter to your favorite Women. Here you can see images for photos of most pupular Ukrainian Women for dating and mariage.
The process of forgetting someone you loved can break you. Or it can transform you into a stronger, more balanced and more mature version of yourself, with a much higher potential for choosing and creating deeply fulfilling relationships in the future. Here are 7 basic principles you should keep in mind as you strive to forget someone you loved. How to forget someone — Rule 1. It makes us always available to them as a fall back option.
Photo by Staydazzled How to forget someone — Rule 2. The key is to shift your focus away from them instead. Now is a great time to take a fresh look at your life. Concentrate on the gifts of singlehood. Re-evaluate your life goals.

tokenized:
What is a ready dating website by Dating Pro Professional dating site in 1 day You pick a package , we do the rest . Full technical documentation from developers for developers . Open to modification by any PHP developer . No “ powered - by , ” no ads unless you want them for making money .
 He wanted to make plans to hang out some time . A specific place and time .
 Paper World offers offers original newspapers , magazines and other memorabilia from the day you were born . Unique gift ideas for a birthday present , Mother ’s Day gift and more birthday gift ideas .
 See some words or phrases that you do n’t understand ? Check out The Dragon ’s Lexicon . My dreams consist of love , laughter , and living life to its fullest . A lover of fine wine and food , I tend to break the bank of those who can handle me .
 He might answer a few emails . Or possibly take a quick call . I have to get going . Often , I stick around without him . What kind of madman voluntarily works on a Friday night ? All of those words sound funny . Like they should be used to describe someone much older . But weirdly , they apply to Rand . And have for years . We go on the road together . The answer really should be no . And yet , when they ask , all I can say is yes . I miss him constantly .
 Always tugging at those damn heartstrings of mine when he leaves a room .
 Check out our members now ! Join free , anonymous and instantly ! Meet new people and start dating ! You can send messages anonymously to anyone you like . Start meeting new people today and discover the real potential of this website . You have probably been waiting for a chance like this – now start to discover how enjoyable life can really be !
 Single marriage - minded women only . We offer 4 , easy to understand , services with one downpayment of Euro for all 4 . We will introduce you to ALL girls from Ukraine in our database meeting your criteria . You will get a list of those who interested and direct contact of 2 ladies wanting you to write them ! Direct contact info of ONE girl : This means she is opened for relationship with you and expecting your contact . You may select several girls to see who likes you and get in touch with just one .
 It was the last time she saw him . I have countless emails from readers telling me stories of guys and women who moved the initial dating period along at high speed .
 Hello I ’ve been in a relationship with a widower for a little over a year . When I met him , it was 3 years since he ’d lost his wife . I was the first girlfriend he ’d had in that time .
 Organizing trip for girl you want to meet in your or third country : Introduction tour to Kiev to meet ALL girls you like : See who has Upcoming Birthday – write a special Birthday letter to your favorite Women . Here you can see images for photos of most pupular Ukrainian Women for dating and mariage .
 The process of forgetting someone you loved can break you . Or it can transform you into a stronger , more balanced and more mature version of yourself , with a much higher potential for choosing and creating deeply fulfilling relationships in the future . Here are 7 basic principles you should keep in mind as you strive to forget someone you loved . How to forget someone — Rule 1 . It makes us always available to them as a fall back option .
 Photo by Staydazzled How to forget someone — Rule 2 . The key is to shift your focus away from them instead . Now is a great time to take a fresh look at your life . Concentrate on the gifts of singlehood . Re - evaluate your life goals .


text:
Throttle cable and tube lil problem help me please.
Discussion in 'The Lounge' started by Adrian bravo, Jun 3, 2018.
Hey Im new to the forum. I have purchased an xs400 I'm new to motorcycles now the last owner of the bike has upgraded the throttle tube, throttle cable, etc. Now I was riding and the cable broke I was looking on the internet but can't find the throttle cable. Only the original, but it won't work because again he upgraded the throttle tube. So I went and bought the original throttle cable and idk what else besides the throttle tube I need since I never seen the original setting. help please!
The stock throttle tube works with the stock handlebar switches, which you don't appear to be using.

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Throttle cable and tube lil problem help me please .
 Discussion in ' The Lounge ' started by Adrian bravo , Jun 3 , 2018 .
 Hey I m new to the forum . I have purchased an xs400 I 'm new to motorcycles now the last owner of the bike has upgraded the throttle tube , throttle cable , etc . Now I was riding and the cable broke I was looking on the internet but ca n't find the throttle cable . Only the original , but it wo n't work because again he upgraded the throttle tube . So I went and bought the original throttle cable and idk what else besides the throttle tube I need since I never seen the original setting . help please !
 The stock throttle tube works with the stock handlebar switches , which you do n't appear to be using .


text:
Sad but true. I used to really enjoy the game. Official Notice Here Odd since it went free to play, but I guess that’s not a guarantee for success either.
Breaking news over at broken toys – the return of an old MMO hero/villain. Is Brad McQuaid back? Myself, as well as others tend to think it’s a hoax. Let’s chat about it a bit – it has been a slow gaming “work” week anyway.

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Sad but true . I used to really enjoy the game . Official Notice Here Odd since it went free to play , but I guess that ’s not a guarantee for success either .
 Breaking news over at broken toys – the return of an old MMO hero / villain . Is Brad McQuaid back ? Myself , as well as others tend to think it ’s a hoax . Let ’s chat about it a bit – it has been a slow gaming “ work ” week anyway .


text:
Notice is hereby given that the Board of Appeal of the Township of Penn, McLeod County, MN will meet at the office of the Clerk in said township at the Penn Township Hall located at 15989 40th Street, in said township at 7:00 p.m. on Monday the 8th day of April 2019 for the purpose of reviewing and correcting the assessment of said township for the year 2020.
All persons considering themselves aggrieved by said assessment, or who wish to complain that the property of another is assessed too low, are hereby notified to appear at said meeting and show cause of having such assessment corrected. No complaint that another person is assessed too low will be acted upon until the person so assessed, or his agent, shall have been notified of such complaint.
Given under my hand this 22nd day of March 2019.

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Notice is hereby given that the Board of Appeal of the Township of Penn , McLeod County , MN will meet at the office of the Clerk in said township at the Penn Township Hall located at 15989 40th Street , in said township at 7:00 p.m. on Monday the 8th day of April 2019 for the purpose of reviewing and correcting the assessment of said township for the year 2020 .
 All persons considering themselves aggrieved by said assessment , or who wish to complain that the property of another is assessed too low , are hereby notified to appear at said meeting and show cause of having such assessment corrected . No complaint that another person is assessed too low will be acted upon until the person so assessed , or his agent , shall have been notified of such complaint .
 Given under my hand this 22nd day of March 2019 .


text:
Proceedings of the KMGov2002 Workshop, Copenhagen, Denmark, May 23-24, 2002, pp. 173-185.
Public transaction services (such as e-forms), although perceived the future of e-government have not yet realised their full potential. E-forms have a significant role in e-government, as they are the basis for realising most of the twenty public services that all European Union member states have to provide to their citizens and businesses. The aim of this paper is to present a knowledge-based platform to assist public sector employees to generate online transaction services by simplifying their development, maintenance and integration with already installed IT systems.

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Proceedings of the KMGov2002 Workshop , Copenhagen , Denmark , May 23 - 24 , 2002 , pp . 173 - 185 .
 Public transaction services ( such as e - forms ) , although perceived the future of e - government have not yet realised their full potential . E - forms have a significant role in e - government , as they are the basis for realising most of the twenty public services that all European Union member states have to provide to their citizens and businesses . The aim of this paper is to present a knowledge - based platform to assist public sector employees to generate online transaction services by simplifying their development , maintenance and integration with already installed IT systems .


text:
Teachers from AOS92 and across Maine learn how to "Code"
Teachers from AOS92 and across Maine gathered at VCS this Saturday for a day of learning how to teach computer science using Code.org. Mike Harvey was the facilitator for 14 educators who spent the day conducting sample "unplugged" lessons, exploring computer science for young learners, and trying out the lessons on Code.org. This is the second time that Mike has been invited to VCS to conduct this awesome workshop. Thanks Mike!

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Teachers from AOS92 and across Maine learn how to " Code "
 Teachers from AOS92 and across Maine gathered at VCS this Saturday for a day of learning how to teach computer science using Code.org . Mike Harvey was the facilitator for 14 educators who spent the day conducting sample " unplugged " lessons , exploring computer science for young learners , and trying out the lessons on Code.org . This is the second time that Mike has been invited to VCS to conduct this awesome workshop . Thanks Mike !


text:
“En Construccíon” features works that reveal the dynamic that makes up a collection and investigates the dialogue between poetic possibilities, and political, social and historical questions that arise from the artists´ creations. Curated by Hersilia Álvarez, Camila Feal, Amanda Nascimento, Cecília Rabossi and Juliana Robles de la Pava, Ivan Grilo is one of the 21 international artists that have contributed to the exhibition.
For the first time, the MUNTREF collection features an exhibition that is literally in construction, heterogeneous and with no single criteria. Made up of donations, acquisitions and parts of other temporary showings. In it, there are waves of meaning and works that establish a complex cosmography.
From a point of view that questions the limits of its own present timing, “En Construccíon” proposes a dialogue between expressions of modernity and contemporary production that allows new debates to be activated and new problems to be extrapolated.
Themes from the uses of language, immigration, discussions about the environment and the search to make visible the relations between the visual and the textual, questioning the natural and social environment are present in the array of works presented.

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“ En Construccíon ” features works that reveal the dynamic that makes up a collection and investigates the dialogue between poetic possibilities , and political , social and historical questions that arise from the artists ´ creations . Curated by Hersilia Álvarez , Camila Feal , Amanda Nascimento , Cecília Rabossi and Juliana Robles de la Pava , Ivan Grilo is one of the 21 international artists that have contributed to the exhibition .
 For the first time , the MUNTREF collection features an exhibition that is literally in construction , heterogeneous and with no single criteria . Made up of donations , acquisitions and parts of other temporary showings . In it , there are waves of meaning and works that establish a complex cosmography .
 From a point of view that questions the limits of its own present timing , “ En Construccíon ” proposes a dialogue between expressions of modernity and contemporary production that allows new debates to be activated and new problems to be extrapolated .
 Themes from the uses of language , immigration , discussions about the environment and the search to make visible the relations between the visual and the textual , questioning the natural and social environment are present in the array of works presented .


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Northern Ireland, Scotland, and England.
Hay Tourist Information Bureau, Oxford Road, Hay-on-Wye, Hereford, HR3 5DG.
(Yes, the postal address is England even though they are in Wales.)"
where you can eat while they search for your books.
Buses run to Hay from Hereford and Brecon.  Early closing day is Tuesday.
Blackwells (St. Mary St).  "Good large general bookshop."
secondhand bookshop with an excellent SF section."
of 06/00, one of the branches that carries books.
as well as for those involved in business, commerce, etc.
House, 16 Melbourn, Royston, Herts, SG8 7BZ, 01763-248400, FAX 01763-248921.
ISLES.  The Seventh Edition was published in July 1999.

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Northern Ireland , Scotland , and England .
 Hay Tourist Information Bureau , Oxford Road , Hay - on - Wye , Hereford , HR3 5DG .
 ( Yes , the postal address is England even though they are in Wales . ) "
 where you can eat while they search for your books .
 Buses run to Hay from Hereford and Brecon .   Early closing day is Tuesday .
 Blackwells ( St. Mary St ) .   " Good large general bookshop . "
 secondhand bookshop with an excellent SF section . "
 of 06/00 , one of the branches that carries books .
 as well as for those involved in business , commerce , etc .
 House , 16 Melbourn , Royston , Herts , SG8 7BZ , 01763 - 248400 , FAX 01763 - 248921 .
 ISLES .   The Seventh Edition was published in July 1999 .


text:
But his musical career didn’t take off until he had a dual awakening. About 12 years ago, the Lawrenceville resident renewed his Christian faith and his interest in old-time country music.
The result has been a spiritual and professional rebirth for Forsythe, 60, who lives at Nied’s Hotel, the Lawrenceville establishment famous for its fish sandwiches and live music.
A video, “Live From Nied’s Hotel,” filmed and produced for WQED-TV, earned a Mid-Atlantic Emmy for Arts Program nomination for Forsythe and producer and director Dino DiStefano.
Forsythe’s new album, “This is Slim Forsythe,” is available now via Get Hip Records, which will host a release party at its Pittsburgh’s North Side location on July 1.
Question: How did you get into country music?
Answer: It’s what I’m all about now, but I came to it in the middle of my life. Now, of course, growing up in the second half of the 20th century, you’re going to get some of that by osmosis: “Jambalaya,” “Hey Good Lookin’,” … It was in the air but I didn’t get hooked on it until the early 1990s. The Slim thing came along in 2004, 2005. My first gig as Slim Forsythe — and I say this with classic understatement — we were Slim Forsythe and the Parklane Drifters, and I billed it as “One of Steeltown’s Finest Three-Quarter Female Hank Williams Tribute Bands.” And it was true.
Q: What was it about country music that appealed to you?
A: All I can say that the stuff that really grabbed me was some of the lesser-known works, like the old Hank (Williams) and Audrey Mae Sheppard gospel duets like “Dear Brother,” “Jesus Remembered Me,” “Lost on the River.” That stuff just grabbed me and I started learning every Hank Williams song I could.
Q: The new album is just vocals and guitar, and features tributes to your musical influences including “Mother Queen of My Heart” by Slim Bryant and Jimmie Rodgers, and Roy Acuff’s “Wreck on the Highway.” Can you talk about why you decided to record these tracks?
A: There are five songs where I pay tribute to my heroes. … I do an old song from the 1800s, “Put My Little Shoes Away,” which came into the bluegrass repertoire of the 20th century through Bill Monroe and the Stanley Brothers. That’s side one. On side two I follow in that tradition with my own original stuff. Including new words to “I’ve Been Everywhere” (by Johnny Cash). It’s now “Slim’s Been Everywhere.” …. It’s just me and my guitar, and it’s 100 percent live.
Q: The discovery of this music also led to a spiritual rebirth for you. How did country music connect with your faith?
A: I’ve been a Christian since I was a boy, but I do have to say finding this Slim thing and classic country music was a big part of getting me back to my faith, and on a better path. I had kind of lost my way for a decade or so, and I really gotta say it was music of Hank Williams and Johnny Cash and the Stanley Brothers that helped me find my way back. I don’t think I ever lost my faith, but I did misplace it for a while.
Q: Does your faith come out in your performances?
A: There’s a vibe to the material, it just amazing to see what happens when you throw a gospel song into the mix. I don’t do a lot of straight church work. Once in a while I do and that’s all fine. But I’m the guy that’s singing the gospel song at one a.m. in the saloon the night before. It just seems to be my path.
Q: This is a true solo album, recorded with the masterful Gregg Kostelich at Get Hip. But you’re also known for having some great musicians in your bands.
A: The focus is off that now because this record is just me and Betty, my trusted guitar. But I’ve been very lucky the past 13 years to do shows with Pete Freeman on pedal steel guitar, Tricky Mannion on fiddle. My steady bass player is Candy Mountain (Erin Snyder) who is just a fabulously talented musician. I’ve collaborated with Evan Knauer (of A.T.S.) who has played with me through the years in various iterations. On my best night I am a mediocre rhythm guitarist, but I’ve found a way to be surrounded by these fabulously talented musicians.
The CD release party for “This is Slim Forsythe” is July 1 at Get Hip Records Warehouse, 1800 Preble Ave., Pittsburgh’s North Side. Details: 412-231-4766, gethip.com.
The WYEP Summer Music Festival is now in its 20th year, having hosted artists including Jason Isbell, Sam Roberts, Sinead O’Connor, .moe and Freedy Johnston.

tokenized:
But his musical career did n’t take off until he had a dual awakening . About 12 years ago , the Lawrenceville resident renewed his Christian faith and his interest in old - time country music .
 The result has been a spiritual and professional rebirth for Forsythe , 60 , who lives at Nied ’s Hotel , the Lawrenceville establishment famous for its fish sandwiches and live music .
 A video , “ Live From Nied ’s Hotel , ” filmed and produced for WQED - TV , earned a Mid - Atlantic Emmy for Arts Program nomination for Forsythe and producer and director Dino DiStefano .
 Forsythe ’s new album , “ This is Slim Forsythe , ” is available now via Get Hip Records , which will host a release party at its Pittsburgh ’s North Side location on July 1 .
 Question : How did you get into country music ?
 Answer : It ’s what I ’m all about now , but I came to it in the middle of my life . Now , of course , growing up in the second half of the 20th century , you ’re going to get some of that by osmosis : “ Jambalaya , ” “ Hey Good Lookin ’ , ” … It was in the air but I did n’t get hooked on it until the early 1990s . The Slim thing came along in 2004 , 2005 . My first gig as Slim Forsythe  — and I say this with classic understatement — we were Slim Forsythe and the Parklane Drifters , and I billed it as “ One of Steeltown ’s Finest Three - Quarter Female Hank Williams Tribute Bands . ” And it was true .
 Q : What was it about country music that appealed to you ?
 A : All I can say that the stuff that really grabbed me was some of the lesser - known works , like the old Hank ( Williams ) and Audrey Mae Sheppard gospel duets like “ Dear Brother , ” “ Jesus Remembered Me , ” “ Lost on the River . ” That stuff just grabbed me and I started learning every Hank Williams song I could .
 Q : The new album is just vocals and guitar , and features tributes to your musical influences including “ Mother Queen of My Heart ” by Slim Bryant and Jimmie Rodgers , and Roy Acuff ’s “ Wreck on the Highway . ” Can you talk about why you decided to record these tracks ?
 A : There are five songs where I pay tribute to my heroes . … I do an old song from the 1800s , “ Put My Little Shoes Away , ” which came into the bluegrass repertoire of the 20th century through Bill Monroe and the Stanley Brothers . That ’s side one . On side two I follow in that tradition with my own original stuff . Including new words to “ I ’ve Been Everywhere ” ( by Johnny Cash ) . It ’s now “ Slim ’s Been Everywhere . ” … . It ’s just me and my guitar , and it ’s 100 percent live .
 Q : The discovery of this music also led to a spiritual rebirth for you . How did country music connect with your faith ?
 A : I ’ve been a Christian since I was a boy , but I do have to say finding this Slim thing and classic country music was a big part of getting me back to my faith , and on a better path . I had kind of lost my way for a decade or so , and I really got ta say it was music of Hank Williams and Johnny Cash and the Stanley Brothers that helped me find my way back . I do n’t think I ever lost my faith , but I did misplace it for a while .
 Q : Does your faith come out in your performances ?
 A : There ’s a vibe to the material , it just amazing to see what happens when you throw a gospel song into the mix . I do n’t do a lot of straight church work . Once in a while I do and that ’s all fine . But I ’m the guy that ’s singing the gospel song at one a.m. in the saloon the night before . It just seems to be my path .
 Q : This is a true solo album , recorded with the masterful Gregg Kostelich at Get Hip . But you ’re also known for having some great musicians in your bands .
 A : The focus is off that now because this record is just me and Betty , my trusted guitar . But I ’ve been very lucky the past 13 years to do shows with Pete Freeman on pedal steel guitar , Tricky Mannion on fiddle . My steady bass player is Candy Mountain ( Erin Snyder ) who is just a fabulously talented musician . I ’ve collaborated with Evan Knauer ( of A.T.S. ) who has played with me through the years in various iterations . On my best night I am a mediocre rhythm guitarist , but I ’ve found a way to be surrounded by these fabulously talented musicians .
 The CD release party for “ This is Slim Forsythe ” is July 1 at Get Hip Records Warehouse , 1800 Preble Ave . , Pittsburgh ’s North Side . Details : 412 - 231 - 4766 , gethip.com .
 The WYEP Summer Music Festival is now in its 20th year , having hosted artists including Jason Isbell , Sam Roberts , Sinead O’Connor , .moe and Freedy Johnston .


text:
C-Reactive protein (CRP), the prototypical acute-phase protein, is produced by liver hepatocytes and regulated by cytokines, particularly interleukin-6 (1)(2). Circulating concentrations of CRP indicate inflammatory activity, and the recent development of highly sensitive CRP assays (3)(4)(5) has led to the discovery that slight increases in CRP (>1–2 mg/L) are indicative of low-grade inflammatory processes that may be related to the pathophysiology of cardiovascular disease. More than a dozen population-based studies have demonstrated that increased CRP is an independent risk factor for future cardiovascular disease, with adjusted odds ratios >2.0 (6)(7)(8)(9). The American Heart Association and the CDC have recommended measurements of CRP in clinical practice and called for additional population-based research (10).
A potential obstacle to the measurement of CRP (as well as other biomarkers) in large epidemiologic, community-based studies is the requirement for venous blood. Venipuncture is a relatively invasive procedure that must be performed by a trained phlebotomist (usually in a clinical setting), and it requires readily accessible facilities where blood samples can be promptly processed and stored under controlled conditions. Assays using whole blood dried on filter paper may provide a viable alternative: Several community-based applications have shown this to be a convenient and reliable means to facilitate sample collection, storage, and transportation, and laboratory methods have been validated for a growing number of analytes (11)(12)(13)(14)(15)(16). “Guthrie papers” have been a core component of US hospital-based newborn-screening programs since the 1960s and are subject to a rigorous quality-control program (17).
Samples can be collected on filter paper easily by nonmedical personnel: The patient’s finger is pricked with a sterile, disposable lancet (commonly used by diabetics), and up to five drops of blood (∼50 μL per drop) are spotted onto standardized filter paper (no. 903; Schleicher and Schuell) that is certified to meet performance standards for sample absorption and lot-to-lot consistency set by the NCCLS and by the Food and Drug Administration regulations for Class II Medical Devices. The samples are allowed to dry and then shipped by express or standard mail to the laboratory for freezer storage.
We describe a high-sensitivity enzyme immunoassay for CRP in dried blood spots. To minimize matrix differences and maximize comparability between calibrators and unknowns, dried-blood-spot calibrators were made by diluting delipidated human serum enriched with CRP [X0923 (standardized against the WHO International Reference Preparation); Dako] with washed erythrocytes, followed by application to filter paper. Washed erythrocytes were obtained as follows: (a) Whole blood was collected by venipuncture in 5-mL EDTA Vacutainer Tubes and centrifuged at 1500g for 15 min. (b) Plasma and buffy coat were removed and discarded. (c) Approximately 3 mL of saline (0.86 g of NaCl in 100 mL of deionized H2O) was added. (d) Tubes were mixed gently for 5 min on a hematology rotor and centrifuged as before. Saline and any remaining buffy coat were removed, and steps c and d were repeated for a total of three washes. The CRP preparation was serially diluted in assay buffer (0.01 mol/L phosphate buffer, 0.5 mol/L NaCl, pH 7.2), diluted 1:2 with washed erythrocytes, and pipetted on filter paper in 50-μL drops. Final calibrator concentrations were 10.1, 5.07, 2.53, 1.27, 0.63, 0.32, 0.16, 0.08, and 0.00 mg/L.
Microtiter plates (cat. no. 439454; NUNC Maxisorp) were coated overnight with 100 μL/well rabbit anti-human CRP antibody (cat. no. A0073; Dako) at a concentration of 10 mg/L in coating buffer (0.01 mol/L phosphate buffer, 0.145 mol/L NaCl, 0.1 g/L Thimerosal, pH 7.2). One 3.2-mm disk from each blood spot sample, control, and calibrator was eluted overnight at 4 °C in 250 μL of wash/elution buffer (0.01 mol/L phosphate buffer, 0.5 mol/L NaCl, 1 mL/L Tween 20, pH 7.2) and rotated on a microplate shaker (cat. no. 51402; Cole-Parmer; 3 mm orbit) at 300 rpm at room temperature for 60 min the following day. Eluate (100 μL) from each disk was pipetted in duplicate into microtiter wells that had been blocked by incubation for 30 min with wash/elution buffer. Wells were washed after a 2-h incubation at room temperature, with rotation at 250 rpm. Detection antibody (peroxidase-conjugated rabbit anti-human CRP antibody; cat. no. P227; Dako) was diluted to 0.163 mg/L in wash/elution buffer, added to the wells (100 μL), and incubated for 2 h at room temperature. The wells were washed, and 100 μL of chromogenic substrate [14 mg of 1,2-phenylenediamine dihydrochloride (cat. no. S2045; Dako), 5 μL of 300 g/L H2O2, and 12 mL of deionized H2O) were added for color development. Wells were incubated in the dark for 30 min before the addition of 100 μL of stop solution (0.5 mol/L H2SO4). The absorbance was read at 490 nm (BioTek Elx808), and sample concentrations were calculated from the best-fit four-parameter logistic calibration curve (KCJunior; BioTek). Samples reading above the highest calibrator were reanalyzed at a higher dilution factor (i.e., disks were eluted in 500 μL of wash/elution buffer and the result was multiplied by 2).
The detection limit (defined as the concentration corresponding to the absorbance 2 SD above the mean of 10 replicates of the 0.00 mg/L calibrator) was 0.028 mg/L. Within-assay imprecision estimates (CV) at 1.01 and 5.09 mg/L were 5.1% and 6.4% (n = 10 determinations), respectively; between-assay CV were 9.5% and 6.9%, respectively (n = 10 determinations across 10 assays performed on different days). We investigated assay linearity by serially diluting two samples after elution. The observed values ranged from 94.5% to 109% of expected, with a mean of 103%. Control sera containing a known concentration of purified CRP (cat. no. X0925; Dako) were diluted 1:2 with washed erythrocytes and spotted on filter paper. Observed values for the low and high control samples were 102% and 93% of expected, respectively.
We compared CRP concentrations in 94 paired blood-spot and serum samples (Fig. 1⇓ ) that were leftover patient samples. Serum samples were analyzed with a turbidimetric assay on the IMMAGE™ Immunochemistry System (Beckman Coulter, Inc). The relationship between the blood spot and serum methods was linear, and the correlation was high. The regression equation provides a means for generating serum equivalents from blood-spot samples if desired (12), although a larger, more representative sample of paired blood-spot and serum samples should be enlisted to generate this equation before its application. We also compared serum and blood-spot results by calculating the ratio of serum CRP to blood-spot CRP and inspecting for differences or inconsistent variability across the measurement range (18). The mean (SD) ratio was 1.39 (0.48), with two values outside the 95% limits of agreement. These values belonged to samples with blood-spot CRP concentrations <0.5 mg/L and reflect the slightly higher variability in the agreement between serum and blood spot results for samples <1.0 mg/L. There was no evidence of systematic differences in the serum:blood-spot CRP ratio across the assay range.
Hematocrit correction does not improve agreement between plasma and blood-spot results for gonadotropins and is not necessary for samples with normal hematocrits (12). To confirm this for CRP, we added washed erythrocytes (in concentrations of 30%, 40%, and 50%) to three plasma samples and spotted them on filter paper. There was no consistent association between hematocrit and blood-spot CRP concentration.
Many epidemiologic analyses of the association between serum CRP and cardiovascular disease risk are categorical, with the distribution of CRP concentrations divided into tertiles or quartiles (7)(9). Recently, cutpoints of low risk (<1.0 mg/L), average risk (1.0–3.0 mg/L), and high risk (>3.0 mg/L) have been proposed that approximate the tertile distribution of serum/plasma CRP in a range of populations (10). We compared category assignments according to the blood-spot and serum methods in our 94 matched samples, with the distributions of blood-spot and serum CRP concentrations divided separately into tertiles. Eighty-seven of 94 individuals (93%) were assigned to the same category by both methods.
We evaluated the stability of CRP in dried blood spots by exposing nine samples sealed in plastic bags with two desiccant packs (cat. no. 61161-319; VWR) to the following temperature conditions for up to 14 days: 37 °C, room temperature (21–23 °C), and 4 °C. An additional variable-temperature condition was evaluated (12 h at 32 °C and 12 h at 22 °C) to simulate ambient temperature exposures in tropical environments. Samples were considered to remain stable as long as CRP concentrations remained within 2 SD of baseline values measured in samples stored at −30 °C immediately after collection. Six determinations were used to calculate the baseline mean (SD) for each sample: 1.11 (0.10), 1.58 (0.093), 1.60 (0.15), 2.46 (0.25), 2.65 (0.17), 4.28 (0.17), 4.57 (0.32), 6.40 (0.55), and 6.64 (0.44) mg/L. CRP concentrations remained stable in dried blood spots for 3 days at 37 °C, 3 days at 32/22 °C, and for at least 14 days at room temperature and 4 °C. The stability of CRP to repeated cycles of freezing and thawing was also evaluated, with no evidence of deterioration after five freeze–thaw cycles (1 h at room temperature, repeated over 5 different days).
Previous applications of whole-blood-spot methods have demonstrated performance characteristics similar to those for methods that rely on venipuncture (17). The ease of finger stick blood collection alleviates constraints associated with sampling in clinical settings, increases the frequency with which samples can be taken, and expands the methodologic options for population-level health research. In particular, our high-sensitivity CRP method could potentially be a useful tool for community-based, epidemiologic investigations of inflammation and cardiovascular risk.
Relationship between blood-spot and serum CRP concentrations in 84 paired samples.
The best-fit linear regression line is shown. Samples above the highest blood-spot calibrator (10.13 mg/L) are not included (n = 10).
This study was approved by the Institutional Review Board of the Evanston Northwestern Healthcare Research Institute. Financial support provided by the National Science Foundation (BCS-0134225).
Ballou SP, Kushner I. C-Reactive protein and the acute phase response. Adv Intern Med 1992;37:313-336.
Pepys MB. The acute phase response and C-reactive protein. Weatherall DJ Ledingham JGG Warrell DA eds. Oxford textbook of medicine, 3rd ed 1995;Vol. 2:1527-1533 Oxford University Press Oxford. .
Wilkins J, Gallimore JR, Moore EG, Pepys MB. Rapid automated high sensitivity enzyme immunoassay of C-reactive protein. Clin Chem 1998;44:1358-1361.
Lagrand WK, Visser CA, Hermens WT, Niessen HWM, Verheugt FWA, Wolbink GJ, et al. C-Reactive protein as a cardiovascular risk factor: more than a epiphenomenon?. Circulation 1999;100:96-102.
Danesh J, Whincup P, Walker M, Lennon L, Thomson A, Appleby P, et al. Low grade inflammation and coronary heart disease: prospective study and updated meta-analyses. BMJ 2000;321:199-204.
Pearson TA, Mensah GA, Alexander RW, Anderson JL, Cannon RO, Criqui M, et al. Markers of inflammation and cardiovascular disease: application to clinical and public health practice. Circulation 2003;107:499-511.
McDade TW, Stallings JF, Angold A, Costello EJ, Burleson M, Cacioppo JT, et al. Epstein-Barr virus antibodies in whole blood spots: a minimally-invasive method for assessing an aspect of cell-mediated immunity. Psychosom Med 2000;62:560-567.
Worthman CM, Stallings JF. Measurement of gonadotropins in dried blood spots. Clin Chem 1994;40:448-453.
Worthman CM, Stallings JF. Hormone measures in finger-prick blood spot samples: new field methods for reproductive endocrinology. Am J Physical Anthropol 1997;104:1-22.
Cook JD, Flowers CH, Skikne BS. An assessment of dried blood-spot technology for identifying iron deficiency. Blood 1998;92:1807-1813.
Erhardt JG, Craft NE, Heinrich F, Bielaski HK. Rapid and simple measurement of retinol in dried whole blood spots. J Nutr 2002;132:318-321.
McDade TW, Shell-Duncan B. Whole blood collected on filter paper provides a minimally-invasive method for assessing transferrin receptor level. J Nutr 2002;132:3760-3763.

tokenized:
C - Reactive protein ( CRP ) , the prototypical acute - phase protein , is produced by liver hepatocytes and regulated by cytokines , particularly interleukin-6 ( 1)(2 ) . Circulating concentrations of CRP indicate inflammatory activity , and the recent development of highly sensitive CRP assays ( 3)(4)(5 ) has led to the discovery that slight increases in CRP ( > 1–2 mg / L ) are indicative of low - grade inflammatory processes that may be related to the pathophysiology of cardiovascular disease . More than a dozen population - based studies have demonstrated that increased CRP is an independent risk factor for future cardiovascular disease , with adjusted odds ratios > 2.0 ( 6)(7)(8)(9 ) . The American Heart Association and the CDC have recommended measurements of CRP in clinical practice and called for additional population - based research ( 10 ) .
 A potential obstacle to the measurement of CRP ( as well as other biomarkers ) in large epidemiologic , community - based studies is the requirement for venous blood . Venipuncture is a relatively invasive procedure that must be performed by a trained phlebotomist ( usually in a clinical setting ) , and it requires readily accessible facilities where blood samples can be promptly processed and stored under controlled conditions . Assays using whole blood dried on filter paper may provide a viable alternative : Several community - based applications have shown this to be a convenient and reliable means to facilitate sample collection , storage , and transportation , and laboratory methods have been validated for a growing number of analytes ( 11)(12)(13)(14)(15)(16 ) . “ Guthrie papers ” have been a core component of US hospital - based newborn - screening programs since the 1960s and are subject to a rigorous quality - control program ( 17 ) .
 Samples can be collected on filter paper easily by nonmedical personnel : The patient ’s finger is pricked with a sterile , disposable lancet ( commonly used by diabetics ) , and up to five drops of blood ( ∼50 μL per drop ) are spotted onto standardized filter paper ( no . 903 ; Schleicher and Schuell ) that is certified to meet performance standards for sample absorption and lot - to - lot consistency set by the NCCLS and by the Food and Drug Administration regulations for Class II Medical Devices . The samples are allowed to dry and then shipped by express or standard mail to the laboratory for freezer storage .
 We describe a high - sensitivity enzyme immunoassay for CRP in dried blood spots . To minimize matrix differences and maximize comparability between calibrators and unknowns , dried - blood - spot calibrators were made by diluting delipidated human serum enriched with CRP [ X0923 ( standardized against the WHO International Reference Preparation ) ; Dako ] with washed erythrocytes , followed by application to filter paper . Washed erythrocytes were obtained as follows : ( a ) Whole blood was collected by venipuncture in 5 - mL EDTA Vacutainer Tubes and centrifuged at 1500 g for 15 min . ( b ) Plasma and buffy coat were removed and discarded . ( c ) Approximately 3 mL of saline ( 0.86 g of NaCl in 100 mL of deionized H2O ) was added . ( d ) Tubes were mixed gently for 5 min on a hematology rotor and centrifuged as before . Saline and any remaining buffy coat were removed , and steps c and d were repeated for a total of three washes . The CRP preparation was serially diluted in assay buffer ( 0.01 mol / L phosphate buffer , 0.5 mol / L NaCl , pH 7.2 ) , diluted 1:2 with washed erythrocytes , and pipetted on filter paper in 50 - μL drops . Final calibrator concentrations were 10.1 , 5.07 , 2.53 , 1.27 , 0.63 , 0.32 , 0.16 , 0.08 , and 0.00 mg / L.
 Microtiter plates ( cat . no . 439454 ; NUNC Maxisorp ) were coated overnight with 100 μL / well rabbit anti - human CRP antibody ( cat . no . A0073 ; Dako ) at a concentration of 10 mg / L in coating buffer ( 0.01 mol / L phosphate buffer , 0.145 mol / L NaCl , 0.1 g / L Thimerosal , pH 7.2 ) . One 3.2 - mm disk from each blood spot sample , control , and calibrator was eluted overnight at 4 ° C in 250 μL of wash / elution buffer ( 0.01 mol / L phosphate buffer , 0.5 mol / L NaCl , 1 mL / L Tween 20 , pH 7.2 ) and rotated on a microplate shaker ( cat . no . 51402 ; Cole - Parmer ; 3 mm orbit ) at 300 rpm at room temperature for 60 min the following day . Eluate ( 100 μL ) from each disk was pipetted in duplicate into microtiter wells that had been blocked by incubation for 30 min with wash / elution buffer . Wells were washed after a 2 - h incubation at room temperature , with rotation at 250 rpm . Detection antibody ( peroxidase - conjugated rabbit anti - human CRP antibody ; cat . no . P227 ; Dako ) was diluted to 0.163 mg / L in wash / elution buffer , added to the wells ( 100 μL ) , and incubated for 2 h at room temperature . The wells were washed , and 100 μL of chromogenic substrate [ 14 mg of 1,2 - phenylenediamine dihydrochloride ( cat . no . S2045 ; Dako ) , 5 μL of 300 g / L H2O2 , and 12 mL of deionized H2O ) were added for color development . Wells were incubated in the dark for 30 min before the addition of 100 μL of stop solution ( 0.5 mol / L H2SO4 ) . The absorbance was read at 490 nm ( BioTek Elx808 ) , and sample concentrations were calculated from the best - fit four - parameter logistic calibration curve ( KCJunior ; BioTek ) . Samples reading above the highest calibrator were reanalyzed at a higher dilution factor ( i.e. , disks were eluted in 500 μL of wash / elution buffer and the result was multiplied by 2 ) .
 The detection limit ( defined as the concentration corresponding to the absorbance 2 SD above the mean of 10 replicates of the 0.00 mg / L calibrator ) was 0.028 mg / L. Within - assay imprecision estimates ( CV ) at 1.01 and 5.09 mg / L were 5.1 % and 6.4 % ( n = 10 determinations ) , respectively ; between - assay CV were 9.5 % and 6.9 % , respectively ( n = 10 determinations across 10 assays performed on different days ) . We investigated assay linearity by serially diluting two samples after elution . The observed values ranged from 94.5 % to 109 % of expected , with a mean of 103 % . Control sera containing a known concentration of purified CRP ( cat . no . X0925 ; Dako ) were diluted 1:2 with washed erythrocytes and spotted on filter paper . Observed values for the low and high control samples were 102 % and 93 % of expected , respectively .
 We compared CRP concentrations in 94 paired blood - spot and serum samples ( Fig . 1 ⇓ ) that were leftover patient samples . Serum samples were analyzed with a turbidimetric assay on the IMMAGE ™ Immunochemistry System ( Beckman Coulter , Inc ) . The relationship between the blood spot and serum methods was linear , and the correlation was high . The regression equation provides a means for generating serum equivalents from blood - spot samples if desired ( 12 ) , although a larger , more representative sample of paired blood - spot and serum samples should be enlisted to generate this equation before its application . We also compared serum and blood - spot results by calculating the ratio of serum CRP to blood - spot CRP and inspecting for differences or inconsistent variability across the measurement range ( 18 ) . The mean ( SD ) ratio was 1.39 ( 0.48 ) , with two values outside the 95 % limits of agreement . These values belonged to samples with blood - spot CRP concentrations < 0.5 mg / L and reflect the slightly higher variability in the agreement between serum and blood spot results for samples < 1.0 mg / L. There was no evidence of systematic differences in the serum : blood - spot CRP ratio across the assay range .
 Hematocrit correction does not improve agreement between plasma and blood - spot results for gonadotropins and is not necessary for samples with normal hematocrits ( 12 ) . To confirm this for CRP , we added washed erythrocytes ( in concentrations of 30 % , 40 % , and 50 % ) to three plasma samples and spotted them on filter paper . There was no consistent association between hematocrit and blood - spot CRP concentration .
 Many epidemiologic analyses of the association between serum CRP and cardiovascular disease risk are categorical , with the distribution of CRP concentrations divided into tertiles or quartiles ( 7)(9 ) . Recently , cutpoints of low risk ( < 1.0 mg / L ) , average risk ( 1.0–3.0 mg / L ) , and high risk ( > 3.0 mg / L ) have been proposed that approximate the tertile distribution of serum / plasma CRP in a range of populations ( 10 ) . We compared category assignments according to the blood - spot and serum methods in our 94 matched samples , with the distributions of blood - spot and serum CRP concentrations divided separately into tertiles . Eighty - seven of 94 individuals ( 93 % ) were assigned to the same category by both methods .
 We evaluated the stability of CRP in dried blood spots by exposing nine samples sealed in plastic bags with two desiccant packs ( cat . no . 61161 - 319 ; VWR ) to the following temperature conditions for up to 14 days : 37 ° C , room temperature ( 21–23 ° C ) , and 4 ° C . An additional variable - temperature condition was evaluated ( 12 h at 32 ° C and 12 h at 22 ° C ) to simulate ambient temperature exposures in tropical environments . Samples were considered to remain stable as long as CRP concentrations remained within 2 SD of baseline values measured in samples stored at −30 ° C immediately after collection . Six determinations were used to calculate the baseline mean ( SD ) for each sample : 1.11 ( 0.10 ) , 1.58 ( 0.093 ) , 1.60 ( 0.15 ) , 2.46 ( 0.25 ) , 2.65 ( 0.17 ) , 4.28 ( 0.17 ) , 4.57 ( 0.32 ) , 6.40 ( 0.55 ) , and 6.64 ( 0.44 ) mg / L. CRP concentrations remained stable in dried blood spots for 3 days at 37 ° C , 3 days at 32/22 ° C , and for at least 14 days at room temperature and 4 ° C . The stability of CRP to repeated cycles of freezing and thawing was also evaluated , with no evidence of deterioration after five freeze – thaw cycles ( 1 h at room temperature , repeated over 5 different days ) .
 Previous applications of whole - blood - spot methods have demonstrated performance characteristics similar to those for methods that rely on venipuncture ( 17 ) . The ease of finger stick blood collection alleviates constraints associated with sampling in clinical settings , increases the frequency with which samples can be taken , and expands the methodologic options for population - level health research . In particular , our high - sensitivity CRP method could potentially be a useful tool for community - based , epidemiologic investigations of inflammation and cardiovascular risk .
 Relationship between blood - spot and serum CRP concentrations in 84 paired samples .
 The best - fit linear regression line is shown . Samples above the highest blood - spot calibrator ( 10.13 mg / L ) are not included ( n = 10 ) .
 This study was approved by the Institutional Review Board of the Evanston Northwestern Healthcare Research Institute . Financial support provided by the National Science Foundation ( BCS-0134225 ) .
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 Wilkins J , Gallimore JR , Moore EG , Pepys MB . Rapid automated high sensitivity enzyme immunoassay of C - reactive protein . Clin Chem 1998;44:1358 - 1361 .
 Lagrand WK , Visser CA , Hermens WT , Niessen HWM , Verheugt FWA , Wolbink GJ , et al . C - Reactive protein as a cardiovascular risk factor : more than a epiphenomenon ? . Circulation 1999;100:96 - 102 .
 Danesh J , Whincup P , Walker M , Lennon L , Thomson A , Appleby P , et al . Low grade inflammation and coronary heart disease : prospective study and updated meta - analyses . BMJ 2000;321:199 - 204 .
 Pearson TA , Mensah GA , Alexander RW , Anderson JL , Cannon RO , Criqui M , et al . Markers of inflammation and cardiovascular disease : application to clinical and public health practice . Circulation 2003;107:499 - 511 .
 McDade TW , Stallings JF , Angold A , Costello EJ , Burleson M , Cacioppo JT , et al . Epstein - Barr virus antibodies in whole blood spots : a minimally - invasive method for assessing an aspect of cell - mediated immunity . Psychosom Med 2000;62:560 - 567 .
 Worthman CM , Stallings JF . Measurement of gonadotropins in dried blood spots . Clin Chem 1994;40:448 - 453 .
 Worthman CM , Stallings JF . Hormone measures in finger - prick blood spot samples : new field methods for reproductive endocrinology . Am J Physical Anthropol 1997;104:1 - 22 .
 Cook JD , Flowers CH , Skikne BS . An assessment of dried blood - spot technology for identifying iron deficiency . Blood 1998;92:1807 - 1813 .
 Erhardt JG , Craft NE , Heinrich F , Bielaski HK . Rapid and simple measurement of retinol in dried whole blood spots . J Nutr 2002;132:318 - 321 .
 McDade TW , Shell - Duncan B. Whole blood collected on filter paper provides a minimally - invasive method for assessing transferrin receptor level . J Nutr 2002;132:3760 - 3763 .


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The words , design , placement and materials work together to send a message . The sign masters at April Graphics work with you to determine what installation methods and materials will work best to meet your objectives . We can use your design , or we can design a custom signage solution that fits your specifications .
 By using digital technology , we can transfer your design with high resolution quality onto the substrate that works best for your specific outdoor banner needs . Substrates range from sturdy , weather - resistant fabrics to lighter - weight fabrics that respond to the slightest movement of air . Construction techniques include grommets and pole pockets , sewing and welding .
 GET NOTICED by using a large banner to advertise your new project .


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