text: If you didn’t watch the first season, fire up the netty right now and crush that first season out because it’s GOLD. This is a hot take on the original “Sabrina the Teenage Witch”, it’s a bit more unruly and just darker overall as a tv show. Keirnan Shipka is your leading lady, she plays the role of Sabrina! Check out her transformation in season 2, I honestly can’t wait.

tokenized: If you did n’t watch the first season , fire up the netty right now and crush that first season out because it ’s GOLD . This is a hot take on the original “ Sabrina the Teenage Witch ” , it ’s a bit more unruly and just darker overall as a tv show . Keirnan Shipka is your leading lady , she plays the role of Sabrina ! Check out her transformation in season 2 , I honestly ca n’t wait .


text:
Landscape, nature and wildlife photography and footage of best worldwide locations. Monuments and architecture photos and videos from capitals cities.
11,191 stock photos, vectors, and illustrations are available royalty-free.
ILLUSTRATION: Visual representation of first picture of a Black Hole, revealed on 10th April 2019 by the scientists. Black hole at the center of galaxy Messier 87, or M87. background wth copy space.
Panorama of Coronado old pier reflecting on in San Diego Bay from Coronado Island, California, USA. San Diego cityscape skyline with Downtown and Waterfront Marina District at twilight on background.
Singapore - May 5, 2018: Google sign in the new offices of Google Headquarters in Mapletree Business City II, Singapore. Google's Asia-Pacific HQ with employs 1000 people.
Wild deer in Nara Park in Japan. Deer are symbol of Nara's greatest tourist attraction. On background, red Torii gate of Kasuga Taisha Shine one of the most popular temples in Nara City.
Franschhoek Wine Tram hop-on hop-off tour, one of the best ways to discover Franschhoek Valley in scenic landscape of Wine Region, near Cape Town, South Africa.
Singapore - May 5, 2018: closeup of Google sign inside a new office to house fast growing team of engineers in Singapore. Google's Asia-Pacific Headquarters con employs 1000 people.
Sunset light on Douro River from Serra do Pilar at Vila Nova de Gaia, Oporto, POrtugal in Europe. Dom Luis I is the most iconic and famous of these bridges.
Milan, Italy - November 15, 2016: refectory of church Santa Maria Delle Grazie in Milan in Italy, hosting The Last Supper masterpiece of late 1490s, after restoration. right side point of view.
Scenic aerial view of Clarke Quay and Riverside area at blue hour in Singapore, Southeast Asia. Waterfront skyline reflected on Singapore River. Popular attraction for nightlife.
Tourism in Algarve. Summer holidays in Portugal, Europe. Lifestyle tourist sitting on promontory of Ponta da Piedade. Caucasian woman looking amazing views of iconic cliffs of turquoise sea of Lagos.
Los Gatos, California, United States - August 12, 2018: Netflix Logo at Netflix Headquarters in Silicon Valley, Ca. Netflix is an entertainment service provider for movies and tv.
Sunnyvale, California, USA - August 13, 2018: closeup of Amazon Sign at Enterprise Way, the Big Amazon campus in Sunnyvale, Silicon Valley. Amazon is leader in electronic commerce.
Singapore - May 3, 2018: Infinity Pool at sunset of Skypark that tops the Marina Bay Sands Hotel and Casino from rooftop of La Vie Club Lounge on 57th floor. Financial district skyline on background.
Los Angeles, California, United States - August 9, 2018: aerial view of tourists take photo onpanoramic terrace at twilight. People look at LA Downtown skyline from Oue Skyspace U.S. Bank Tower.
Perth, Australia - Jan 6, 2018: turistic ferry in Elizabeth Quay Marina at sunset with skyscrapers of Central Business District on background in Perth, Western Australia.
Los Angeles, California, United States - August 9, 2018: La Luz Del Dia Restaurant and La Plaza United Methodist Church in Old Plaza, Olvera Streets, downtown LA, Los Angeles State Historic Landmark.
Macau, China - December 9, 2016: Venetian Macao by night, golden colors of this resort and Casino at night seen from Galaxy Cotai Strip. Gambling tourism is Macau's biggest source of revenue.
Sunnyvale, California, USA - August 13, 2018: closeup of Amazon Sign atop of glass building of Big Amazon campus in Silicon Valley. Amazon is leader in electronic commerce and cloud computing.
Aerial view of Sodwana Bay National Park within the iSimangaliso Wetland Park, Maputaland, an area of KwaZulu-Natal on the east coast of South Africa. Indian Ocean landscape.
Sea chairs in Coronado Beach along Ocean Boulevard on Pacific Ocean, Coronado Island, San Diego. Summer season in West Coast, USA. Blue sky with copy space. Summer holidays in California.
A Sicklefin Lemon shark, Negaprion acutidens, isolated on white background. The Lemon shark lives in the sandy reef near the edge of water. Copy space. View from top.
Oporto, Portugal - August 13, 2017: large wooden staircase with red steps inside Library Lello and Irmao in historic center of Porto, famous for Harry Potter film. Horizontal shot.
Blonde woman on balcony looking canals of Venice, a Venetian style waterfront village in sunny day. Caucasian tourist enjoys Qanat Quartier in the Pearl-Qatar, icon of Doha, Persian Gulf, Middle East.
Singapore - May 2, 2018: Universal Studios world globe in green light, with tourists visiting this Hollywood movie theme park in Sentosa island.
Aerial view of South African hippopotamus family. Cape hippopotamus sleeping on a shore of St Lucia Estuary in iSimangaliso Wetland Park, South Africa. Scenic tourist flight.
Historic Minaret of the Mosque in Katara. Katara is a cultural village also named valley of cultures in Doha, West Bay, Qatar. Middle East, Arabian Peninsula.
Doha, Qatar - February 21, 2019: Khalifa National Stadium the main stadium of Qatar in Aspire Park, will host the 2022 World Cup. Finally completed and renovated, fully covered with air conditioning.
Perth, Australia - Jan 6, 2018: famous landmark in Perth Downtown: Esplanade, skyscrapers of Central Business District, Elizabeth Quay Carousel and Spanda Sculpture in Elizabeth Quay. Sunset light.
Scenic landscape of iconic Santa Monica Pier at orange sunset sky from the beach on Paficif Ocean. Santa Monica Historic Landmark, California, USA. Wide banner panorama.
Flower beds and blooming trees along seafront of Doha Bay in Doha park. Popular tourist attraction near Corniche in city center. Qatar, Middle East, Arabian Peninsula, Persian Gulf. Sunny blue sky.
Front view of portrait of male Greater kudu, a species of antelope at sunset light. Game drive safari in iSimangaliso Wetland Park, South Africa. Tragelaphus Strepsiceros species.
Wild deer and Torii gate of Nara Park in Japan. Deer are Nara's greatest tourist attraction. red Torii gate of Kasuga Taisha Shine one of the most popular temples in Nara City.
Banner astronomical background. Full red moon phases by night. The total phases of the lunar eclipse. Wide panorama.
Doha seafront and West Bay skyline along Corniche in Qatari capital with Dhow Harbour lighting at blue hour. Doha in Qatar. Middle East, Arabian Peninsula, Persian Gulf. Twilight shot.
Close up of Kilauea Volcano, Hawaii Volcanoes National Park, also known Kilauea Smile because from 2016 seems to smile, erupting lava into Pacific Ocean, Big Island. Scenic sea view from the boat.
Decorated entrance gate of Grand Mosque in West Bay area, Doha in Qatar. Qatar State Mosque in arab style, Middle East, Arabian Peninsula. Evening sky.
Abstract business background. Concept of powerful United States American Dollar. United States Flag and bodybuilder shaped USD currency. Financial concept about exchange rate of American dollars.
Solvang, California, United States - August 10, 2018: old clock tower in Solvang historic downtown, Santa Ynez Valley in Santa Barbara County. A Danish Village is a popular tourist attraction.
Doha, Qatar - February 16, 2019: two Arabs standing near fountain of Museum of Islamic Art with skyscrapers of West Bay skyline on background. Middle East, Arabian Peninsula, Persian Gulf.
Nature's Window over Murchison River Gorge in Kalbarri National Park, Western Australia. The red rock sandstone arch is the most iconic natural attractions in WA.
Purebred white Arabian horse running in a paddock in Doha city center, Capital of Qatar. The traditional stables are part of old Souq Waqif market area. Middle East, Arabian Peninsula in Persian Gulf.
Doha, Qatar - February 20, 2019: two heritage Police Officers in traditional 1940s Qatari uniform riding white Arabian Horses at square of Souq Waqif. Popular tourist attraction in Doha city center.
HONOLULU, OAHU, HAWAII, USA - AUGUST 21, 2016: statue portrait of Admiral Chester W. Nimitz, represented the United States during surrender of Japan aboard USS Missouri in Tokyo Bay 1945.
Aerial view of Los Angeles skyline in California, United States. Travel and tourism american concept. Blonde tourist woman looking at Downtown of LA cityscape from observation deck. Sunset shot.
Chacma Baboon mum with baby, species Papio ursinus, sitting on the tree in nature forest. Cape baby baboon hugs mom. Game drive safari in Hluhluwe-iMfolozi Reserve, South Africa. Copy space.
The Capital city of Qatar by night. Doha West Bay skyline reflecting in Doha Bay. Modern glassed skyscrapers of Doha in Qatar, Middle East, Arabian Peninsula in Persian Gulf. Night urban scenary.
Young male tourist feeds with deer biscuit at Nara wild deer in a public park of Nara, Japan. Red Torii and stone lanterns on background. Tourism and travel concept. Spring rain day.
Woman in hat sunbathing on the rocks of Waterfall beach in Denmark, Western Australia. Caucasian tourist looking Great Southern Ocean in William Bay NP. Summer destination in Australia.
Osaka, Japan - April 29, 2017: crowd of people for Golden Week at entrance of Ebisu Bashi-Suji Shopping Street with its colorful neon in Namba District, one of main tourist destinations in Osaka.
Full red moon phases in circle on black background. The total phases of the lunar eclipse turning red.
Panorama of Mount Sinai in Sinai Peninsula of Egypt. Dawn of the holy summit of Mount Sinai, Aka Jebel Musa, know also as Mount of Ten Commandments or Mount of Moses.
Solvang, California, United States - August 10, 2018: people at a coffee shop enjoy picturesque Danish architecture of Solvang. Old Windmill at sunset. Santa Ynez Valley in Santa Barbara County.
Old Windmill in a sunny day with blue sky. Santa Ynez Valley, California, United States. Solvang is a Danish Village, known for its windmills. Popular travel destination. Vertical shot.
Woman with abaya dress looks at views of modern skyscrapers of Doha West Bay skyline outdoors State Grand Mosque in Doha, Qatar, Middle East, Arabian Peninsula. Sunny day with blue sky.
Caucasian woman with raised arms looking at the Al Thakira Mangroves Nature Reserve in Al Dhakira, a popular touristic attraction. Happy blonde tourist visits Qatar in Middle East, Persian Gulf.
Woman with abaya dress looks at views of skyscrapers of Doha West Bay skyline outdoors State Grand Mosque in Doha, Qatar, Middle East, Arabian Peninsula.
Caucasian lifestyle woman enjoying at pond with clear water in Las Vegas Strip. Blonde tourist vacation in Nevada, USA. Las Vegas cityscape daytime. American travel in summer holidays.
Macau, China - December 9, 2016: Canals with Venetian bridge of the Venetian style Casino. Inside luxury shopping mall in the Venetian Hotel in Cotai Strip.
Follow me, woman holding hand at above promontory of Praia Do Camilo in Algarve, Portugal, Europe. Woman freedom at Camilo Beach. Concept of summer holidays and tourist traveler, holding man by hand.
Follow me. Tourist woman with wide hat holding hand of partner looking panorama from terrace over Clarke Quay and Riverside area in Singapore, Southeast Asia. Lady enjoys on Singapore River downtown.
Moon eclipse in full moon and super moon by night. Total Lunar eclipse with moon turning red cause of closer Mars planet. Astronomical background.
Smiling woman with orange sarong and greek clothes enjoying Ancient Theatre Epidaurus amphitheater in Peloponnese, Greece. European travel destination. Historical heritage and landmarks.
Kamakura, Japan - April 23, 2017: Romon the Great Gate at Tsurugaoka Hachiman Shinto shrine.Above the gate is a plaque bearing the name of the shrine, Hachimangu, written in 1629 by Prince Ryoujo.
Woman traveler enjoying at binocular from Oue Skyspace Bank Tower in Los Angeles, California, United States at sunset light. Happy tourist at panoramic terrace above Downtown of LA skyline.
Beach volleyball net at sunset at Fort DeRussy Beach Park which is part of eight sections that make up the popular and long Waikiki Beach in Honolulu, Oahu, Hawaii.
Palm trees along marina walkway in Porto Arabia at the Pearl-Qatar, Doha, with skyscrapers of West Bay skyline at sunset sky. Scenic sunset landscape of Persian Gulf in Middle East.
Albany, Australia - Dec 28, 2017: iconic Albany Town Hall with clock tower is opened in 1888 is the first civic building constructed in Albany, York Street, Western Australia, at twilight.
Carefree woman with raised arms at Welcome to Fabulous Las Vegas Nevada Sign, popular landmark on Las Vegas Strip at entrance of the city. Happy tourist in Nevada, Unites States. Blue sky.
Sweet and tender little australian wombat in position marsupial. Isolated on white background. The Wombat is a crepuscular and nocturnal marsupial.
Spectacular aerial view of Shoshone Falls or Niagara of the West, Snake River, Idaho, United States.
Pillars and sandstone formations seen from Praia do Camilo, Lagos, Algarve, Portugal. Camilo Beach consists of two beaches connected through a tunnel. This part of beach is visible at low tide.
Singapore - May 2, 2018: Universal Studios with luminous globe in Sentosa island at blue hour with pink lights. Universal Studios Singapore is southeast Asia's first Hollywood movie theme park.
Cervantes, Australia - Dec 22, 2017: Four-wheel-drive car on Pinnacles Drive, dirt road in Pinnacles Desert, Nambung National Park, Western Australia. Discovery and adventure travel concept.
Tokyo, Japan - April 17, 2017: green line of Yamanote for Harajuku, the most important train line in Tokyo. Crowd of commuters waiting for rail train at Shinjuku Station in Tokyo.
Rows of grapes in picturesque Stellenbosch, near Cape Town, wine region with Thelema Mountain on backdrop. Stellenbosch Wine Routes are one of most popular attractions of South Africa. Summer season.
Elefant calf protected by mum. Safari game drive in Pilanesberg National Park, South Africa. The African Elephant is part of the Big Five.
Hanami in spring season with unidentifiable women with green Japanese kimono in bamboo grove of Take-dera Hokoku-ji Temple at sunset in Kamakura, Japan city. Japanese culture and lifestyle.
Tourism and travel in California, West Coast. Mobile phone with American flag cover taking photos of San Diego By skyline. Smartphone shoting in summer holidays from Coronado Island, USA.
A couple of Quokka, Setonix brachyurus. Rottnest Island near Perth, Western Australia, is the primary home of the quokka, a type of marsupial. Quokka is considered the happiest animal in the world.

tokenized:
Landscape , nature and wildlife photography and footage of best worldwide locations . Monuments and architecture photos and videos from capitals cities .
 11,191 stock photos , vectors , and illustrations are available royalty - free .
 ILLUSTRATION : Visual representation of first picture of a Black Hole , revealed on 10th April 2019 by the scientists . Black hole at the center of galaxy Messier 87 , or M87 . background wth copy space .
 Panorama of Coronado old pier reflecting on in San Diego Bay from Coronado Island , California , USA . San Diego cityscape skyline with Downtown and Waterfront Marina District at twilight on background .
 Singapore - May 5 , 2018 : Google sign in the new offices of Google Headquarters in Mapletree Business City II , Singapore . Google 's Asia - Pacific HQ with employs 1000 people .
 Wild deer in Nara Park in Japan . Deer are symbol of Nara 's greatest tourist attraction . On background , red Torii gate of Kasuga Taisha Shine one of the most popular temples in Nara City .
 Franschhoek Wine Tram hop - on hop - off tour , one of the best ways to discover Franschhoek Valley in scenic landscape of Wine Region , near Cape Town , South Africa .
 Singapore - May 5 , 2018 : closeup of Google sign inside a new office to house fast growing team of engineers in Singapore . Google 's Asia - Pacific Headquarters con employs 1000 people .
 Sunset light on Douro River from Serra do Pilar at Vila Nova de Gaia , Oporto , POrtugal in Europe . Dom Luis I is the most iconic and famous of these bridges .
 Milan , Italy - November 15 , 2016 : refectory of church Santa Maria Delle Grazie in Milan in Italy , hosting The Last Supper masterpiece of late 1490s , after restoration . right side point of view .
 Scenic aerial view of Clarke Quay and Riverside area at blue hour in Singapore , Southeast Asia . Waterfront skyline reflected on Singapore River . Popular attraction for nightlife .
 Tourism in Algarve . Summer holidays in Portugal , Europe . Lifestyle tourist sitting on promontory of Ponta da Piedade . Caucasian woman looking amazing views of iconic cliffs of turquoise sea of Lagos .
 Los Gatos , California , United States - August 12 , 2018 : Netflix Logo at Netflix Headquarters in Silicon Valley , Ca . Netflix is an entertainment service provider for movies and tv .
 Sunnyvale , California , USA - August 13 , 2018 : closeup of Amazon Sign at Enterprise Way , the Big Amazon campus in Sunnyvale , Silicon Valley . Amazon is leader in electronic commerce .
 Singapore - May 3 , 2018 : Infinity Pool at sunset of Skypark that tops the Marina Bay Sands Hotel and Casino from rooftop of La Vie Club Lounge on 57th floor . Financial district skyline on background .
 Los Angeles , California , United States - August 9 , 2018 : aerial view of tourists take photo onpanoramic terrace at twilight . People look at LA Downtown skyline from Oue Skyspace U.S. Bank Tower .
 Perth , Australia - Jan 6 , 2018 : turistic ferry in Elizabeth Quay Marina at sunset with skyscrapers of Central Business District on background in Perth , Western Australia .
 Los Angeles , California , United States - August 9 , 2018 : La Luz Del Dia Restaurant and La Plaza United Methodist Church in Old Plaza , Olvera Streets , downtown LA , Los Angeles State Historic Landmark .
 Macau , China - December 9 , 2016 : Venetian Macao by night , golden colors of this resort and Casino at night seen from Galaxy Cotai Strip . Gambling tourism is Macau 's biggest source of revenue .
 Sunnyvale , California , USA - August 13 , 2018 : closeup of Amazon Sign atop of glass building of Big Amazon campus in Silicon Valley . Amazon is leader in electronic commerce and cloud computing .
 Aerial view of Sodwana Bay National Park within the iSimangaliso Wetland Park , Maputaland , an area of KwaZulu - Natal on the east coast of South Africa . Indian Ocean landscape .
 Sea chairs in Coronado Beach along Ocean Boulevard on Pacific Ocean , Coronado Island , San Diego . Summer season in West Coast , USA . Blue sky with copy space . Summer holidays in California .
 A Sicklefin Lemon shark , Negaprion acutidens , isolated on white background . The Lemon shark lives in the sandy reef near the edge of water . Copy space . View from top .
 Oporto , Portugal - August 13 , 2017 : large wooden staircase with red steps inside Library Lello and Irmao in historic center of Porto , famous for Harry Potter film . Horizontal shot .
 Blonde woman on balcony looking canals of Venice , a Venetian style waterfront village in sunny day . Caucasian tourist enjoys Qanat Quartier in the Pearl - Qatar , icon of Doha , Persian Gulf , Middle East .
 Singapore - May 2 , 2018 : Universal Studios world globe in green light , with tourists visiting this Hollywood movie theme park in Sentosa island .
 Aerial view of South African hippopotamus family . Cape hippopotamus sleeping on a shore of St Lucia Estuary in iSimangaliso Wetland Park , South Africa . Scenic tourist flight .
 Historic Minaret of the Mosque in Katara . Katara is a cultural village also named valley of cultures in Doha , West Bay , Qatar . Middle East , Arabian Peninsula .
 Doha , Qatar - February 21 , 2019 : Khalifa National Stadium the main stadium of Qatar in Aspire Park , will host the 2022 World Cup . Finally completed and renovated , fully covered with air conditioning .
 Perth , Australia - Jan 6 , 2018 : famous landmark in Perth Downtown : Esplanade , skyscrapers of Central Business District , Elizabeth Quay Carousel and Spanda Sculpture in Elizabeth Quay . Sunset light .
 Scenic landscape of iconic Santa Monica Pier at orange sunset sky from the beach on Paficif Ocean . Santa Monica Historic Landmark , California , USA . Wide banner panorama .
 Flower beds and blooming trees along seafront of Doha Bay in Doha park . Popular tourist attraction near Corniche in city center . Qatar , Middle East , Arabian Peninsula , Persian Gulf . Sunny blue sky .
 Front view of portrait of male Greater kudu , a species of antelope at sunset light . Game drive safari in iSimangaliso Wetland Park , South Africa . Tragelaphus Strepsiceros species .
 Wild deer and Torii gate of Nara Park in Japan . Deer are Nara 's greatest tourist attraction . red Torii gate of Kasuga Taisha Shine one of the most popular temples in Nara City .
 Banner astronomical background . Full red moon phases by night . The total phases of the lunar eclipse . Wide panorama .
 Doha seafront and West Bay skyline along Corniche in Qatari capital with Dhow Harbour lighting at blue hour . Doha in Qatar . Middle East , Arabian Peninsula , Persian Gulf . Twilight shot .
 Close up of Kilauea Volcano , Hawaii Volcanoes National Park , also known Kilauea Smile because from 2016 seems to smile , erupting lava into Pacific Ocean , Big Island . Scenic sea view from the boat .
 Decorated entrance gate of Grand Mosque in West Bay area , Doha in Qatar . Qatar State Mosque in arab style , Middle East , Arabian Peninsula . Evening sky .
 Abstract business background . Concept of powerful United States American Dollar . United States Flag and bodybuilder shaped USD currency . Financial concept about exchange rate of American dollars .
 Solvang , California , United States - August 10 , 2018 : old clock tower in Solvang historic downtown , Santa Ynez Valley in Santa Barbara County . A Danish Village is a popular tourist attraction .
 Doha , Qatar - February 16 , 2019 : two Arabs standing near fountain of Museum of Islamic Art with skyscrapers of West Bay skyline on background . Middle East , Arabian Peninsula , Persian Gulf .
 Nature 's Window over Murchison River Gorge in Kalbarri National Park , Western Australia . The red rock sandstone arch is the most iconic natural attractions in WA .
 Purebred white Arabian horse running in a paddock in Doha city center , Capital of Qatar . The traditional stables are part of old Souq Waqif market area . Middle East , Arabian Peninsula in Persian Gulf .
 Doha , Qatar - February 20 , 2019 : two heritage Police Officers in traditional 1940s Qatari uniform riding white Arabian Horses at square of Souq Waqif . Popular tourist attraction in Doha city center .
 HONOLULU , OAHU , HAWAII , USA - AUGUST 21 , 2016 : statue portrait of Admiral Chester W. Nimitz , represented the United States during surrender of Japan aboard USS Missouri in Tokyo Bay 1945 .
 Aerial view of Los Angeles skyline in California , United States . Travel and tourism american concept . Blonde tourist woman looking at Downtown of LA cityscape from observation deck . Sunset shot .
 Chacma Baboon mum with baby , species Papio ursinus , sitting on the tree in nature forest . Cape baby baboon hugs mom . Game drive safari in Hluhluwe - iMfolozi Reserve , South Africa . Copy space .
 The Capital city of Qatar by night . Doha West Bay skyline reflecting in Doha Bay . Modern glassed skyscrapers of Doha in Qatar , Middle East , Arabian Peninsula in Persian Gulf . Night urban scenary .
 Young male tourist feeds with deer biscuit at Nara wild deer in a public park of Nara , Japan . Red Torii and stone lanterns on background . Tourism and travel concept . Spring rain day .
 Woman in hat sunbathing on the rocks of Waterfall beach in Denmark , Western Australia . Caucasian tourist looking Great Southern Ocean in William Bay NP . Summer destination in Australia .
 Osaka , Japan - April 29 , 2017 : crowd of people for Golden Week at entrance of Ebisu Bashi - Suji Shopping Street with its colorful neon in Namba District , one of main tourist destinations in Osaka .
 Full red moon phases in circle on black background . The total phases of the lunar eclipse turning red .
 Panorama of Mount Sinai in Sinai Peninsula of Egypt . Dawn of the holy summit of Mount Sinai , Aka Jebel Musa , know also as Mount of Ten Commandments or Mount of Moses .
 Solvang , California , United States - August 10 , 2018 : people at a coffee shop enjoy picturesque Danish architecture of Solvang . Old Windmill at sunset . Santa Ynez Valley in Santa Barbara County .
 Old Windmill in a sunny day with blue sky . Santa Ynez Valley , California , United States . Solvang is a Danish Village , known for its windmills . Popular travel destination . Vertical shot .
 Woman with abaya dress looks at views of modern skyscrapers of Doha West Bay skyline outdoors State Grand Mosque in Doha , Qatar , Middle East , Arabian Peninsula . Sunny day with blue sky .
 Caucasian woman with raised arms looking at the Al Thakira Mangroves Nature Reserve in Al Dhakira , a popular touristic attraction . Happy blonde tourist visits Qatar in Middle East , Persian Gulf .
 Woman with abaya dress looks at views of skyscrapers of Doha West Bay skyline outdoors State Grand Mosque in Doha , Qatar , Middle East , Arabian Peninsula .
 Caucasian lifestyle woman enjoying at pond with clear water in Las Vegas Strip . Blonde tourist vacation in Nevada , USA . Las Vegas cityscape daytime . American travel in summer holidays .
 Macau , China - December 9 , 2016 : Canals with Venetian bridge of the Venetian style Casino . Inside luxury shopping mall in the Venetian Hotel in Cotai Strip .
 Follow me , woman holding hand at above promontory of Praia Do Camilo in Algarve , Portugal , Europe . Woman freedom at Camilo Beach . Concept of summer holidays and tourist traveler , holding man by hand .
 Follow me . Tourist woman with wide hat holding hand of partner looking panorama from terrace over Clarke Quay and Riverside area in Singapore , Southeast Asia . Lady enjoys on Singapore River downtown .
 Moon eclipse in full moon and super moon by night . Total Lunar eclipse with moon turning red cause of closer Mars planet . Astronomical background .
 Smiling woman with orange sarong and greek clothes enjoying Ancient Theatre Epidaurus amphitheater in Peloponnese , Greece . European travel destination . Historical heritage and landmarks .
 Kamakura , Japan - April 23 , 2017 : Romon the Great Gate at Tsurugaoka Hachiman Shinto shrine . Above the gate is a plaque bearing the name of the shrine , Hachimangu , written in 1629 by Prince Ryoujo .
 Woman traveler enjoying at binocular from Oue Skyspace Bank Tower in Los Angeles , California , United States at sunset light . Happy tourist at panoramic terrace above Downtown of LA skyline .
 Beach volleyball net at sunset at Fort DeRussy Beach Park which is part of eight sections that make up the popular and long Waikiki Beach in Honolulu , Oahu , Hawaii .
 Palm trees along marina walkway in Porto Arabia at the Pearl - Qatar , Doha , with skyscrapers of West Bay skyline at sunset sky . Scenic sunset landscape of Persian Gulf in Middle East .
 Albany , Australia - Dec 28 , 2017 : iconic Albany Town Hall with clock tower is opened in 1888 is the first civic building constructed in Albany , York Street , Western Australia , at twilight .
 Carefree woman with raised arms at Welcome to Fabulous Las Vegas Nevada Sign , popular landmark on Las Vegas Strip at entrance of the city . Happy tourist in Nevada , Unites States . Blue sky .
 Sweet and tender little australian wombat in position marsupial . Isolated on white background . The Wombat is a crepuscular and nocturnal marsupial .
 Spectacular aerial view of Shoshone Falls or Niagara of the West , Snake River , Idaho , United States .
 Pillars and sandstone formations seen from Praia do Camilo , Lagos , Algarve , Portugal . Camilo Beach consists of two beaches connected through a tunnel . This part of beach is visible at low tide .
 Singapore - May 2 , 2018 : Universal Studios with luminous globe in Sentosa island at blue hour with pink lights . Universal Studios Singapore is southeast Asia 's first Hollywood movie theme park .
 Cervantes , Australia - Dec 22 , 2017 : Four - wheel - drive car on Pinnacles Drive , dirt road in Pinnacles Desert , Nambung National Park , Western Australia . Discovery and adventure travel concept .
 Tokyo , Japan - April 17 , 2017 : green line of Yamanote for Harajuku , the most important train line in Tokyo . Crowd of commuters waiting for rail train at Shinjuku Station in Tokyo .
 Rows of grapes in picturesque Stellenbosch , near Cape Town , wine region with Thelema Mountain on backdrop . Stellenbosch Wine Routes are one of most popular attractions of South Africa . Summer season .
 Elefant calf protected by mum . Safari game drive in Pilanesberg National Park , South Africa . The African Elephant is part of the Big Five .
 Hanami in spring season with unidentifiable women with green Japanese kimono in bamboo grove of Take - dera Hokoku - ji Temple at sunset in Kamakura , Japan city . Japanese culture and lifestyle .
 Tourism and travel in California , West Coast . Mobile phone with American flag cover taking photos of San Diego By skyline . Smartphone shoting in summer holidays from Coronado Island , USA .
 A couple of Quokka , Setonix brachyurus . Rottnest Island near Perth , Western Australia , is the primary home of the quokka , a type of marsupial . Quokka is considered the happiest animal in the world .


text:
John Martinkovic was three-time Pro Bowl selection at defensive end and one of oldest former Packers players.
GREEN BAY – John Martinkovic, one of the oldest surviving former Green Bay Packers players, died Thursday at 91.
Martinkovic, who played defensive end for the Packers from 1951-56, was a three-time Pro Bowl choice and a 1974 inductee into the Green Bay Packers Hall of Fame. He turned 91 on Feb. 4, Super Bowl Sunday.
Drafted by Washington in the sixth round in 1951, Martinkovic was acquired by the Packers in a trade for offensive end Ted Cook. He finished his career in 1957, playing one year for the New York Giants, where he met then-assistant coach Vince Lombardi.
Green Bay Packers historian Cliff Christl pointed out the Packers used the third-round draft pick they got for Martinkovic to draft future Pro Football Hall of Fame linebacker Ray Nitschke.
Martinkovic did not miss a game in his career, playing 84, including 72 in Green Bay.
Martinkovic was a native of Hamilton, Ohio. He served three years in the U.S. Navy before graduating from Xavier University in Cincinnati.
He was a member of four halls of fame, including Badin High (Hamilton Catholic), Butler County and Xavier University, all in Ohio, and the Packers Hall of Fame. He was a member of the NFL Players Association, the NFL Alumni Association, Knights of Columbus Council #617, Green Bay Moose Lodge #801 and Green Bay Retired Men's Club.
He is survived by wife, Clare, four daughters, seven grandchildren and seven great-grandchildren.
Friends may call at St. Agnes Catholic Church, 1484 Ninth St., from 9–11 a.m. Friday, followed by a funeral service.

tokenized:
John Martinkovic was three - time Pro Bowl selection at defensive end and one of oldest former Packers players .
 GREEN BAY – John Martinkovic , one of the oldest surviving former Green Bay Packers players , died Thursday at 91 .
 Martinkovic , who played defensive end for the Packers from 1951 - 56 , was a three - time Pro Bowl choice and a 1974 inductee into the Green Bay Packers Hall of Fame . He turned 91 on Feb. 4 , Super Bowl Sunday .
 Drafted by Washington in the sixth round in 1951 , Martinkovic was acquired by the Packers in a trade for offensive end Ted Cook . He finished his career in 1957 , playing one year for the New York Giants , where he met then - assistant coach Vince Lombardi .
 Green Bay Packers historian Cliff Christl pointed out the Packers used the third - round draft pick they got for Martinkovic to draft future Pro Football Hall of Fame linebacker Ray Nitschke .
 Martinkovic did not miss a game in his career , playing 84 , including 72 in Green Bay .
 Martinkovic was a native of Hamilton , Ohio . He served three years in the U.S. Navy before graduating from Xavier University in Cincinnati .
 He was a member of four halls of fame , including Badin High ( Hamilton Catholic ) , Butler County and Xavier University , all in Ohio , and the Packers Hall of Fame . He was a member of the NFL Players Association , the NFL Alumni Association , Knights of Columbus Council # 617 , Green Bay Moose Lodge # 801 and Green Bay Retired Men 's Club .
 He is survived by wife , Clare , four daughters , seven grandchildren and seven great - grandchildren .
 Friends may call at St. Agnes Catholic Church , 1484 Ninth St. , from 9–11 a.m. Friday , followed by a funeral service .


text:
This exciting twelve (12) credit program introduces students to the basic concepts of health by examining wellness from a holistic perspective. Students are taught how to live healthy lives, manage stress, and understand the benefits of physical activity in addition to learning about complementary and alternative medicine and fitness. If you are looking for a short and informative program that will enhance your personal life or professional practice, you will enjoy taking a course in this program or the entire program to earn a certificate.
Students who wish to take just an individual course from this program as a non-matriculated student, must choose “Continuing Education” on the application page as their program selection. As a non-matriculating student there is NO application fee. Students must supply a photo ID.

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This exciting twelve ( 12 ) credit program introduces students to the basic concepts of health by examining wellness from a holistic perspective . Students are taught how to live healthy lives , manage stress , and understand the benefits of physical activity in addition to learning about complementary and alternative medicine and fitness . If you are looking for a short and informative program that will enhance your personal life or professional practice , you will enjoy taking a course in this program or the entire program to earn a certificate .
 Students who wish to take just an individual course from this program as a non - matriculated student , must choose “ Continuing Education ” on the application page as their program selection . As a non - matriculating student there is NO application fee . Students must supply a photo ID .


text:
But, honestly, it has always been a guiding principle for my professional life. Anytime I have encountered a lack of services for a specific group of people on a particular topic, I have taken it as a hint from the Universe to expand. To grow. To create the resources that did not exist that people so desperately want and need.
Sunstone Health & Wellness is a direct response to a 2013 report in the Indianapolis Star that ranked Indiana as the 8th most overweight state in the United States.
How could I pretend not to see this? To pretend as if the people of my community, my state, my country, were not struggling? To turn a blind eye to the reality that many people are just surviving, and not thriving?
That’s not my style. I want people to opt in to all things at the highest level possible.
Something has to change. We have to change.
My intense love for my work as a therapist, combined with my personal gravitation to all things fresh and healthy, has been the foundation for Sunstone Health & Wellness. I delight in being able to offer people innovative, cutting edge strategies for creating healthy, sustainable change.
Sunstone Health & Wellness is focused on all aspects of health–physical, emotional, mental, and spiritual. With offices in both Greenwood and Indianapolis, we offer convenient locations regardless of where you are in the city.
At Sunstone, we truly love people and respect the journey to living your best life possible. We want to see you grow to live a life of health and vitality. Health for your heart, mind, body, and world at Sunstone.

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But , honestly , it has always been a guiding principle for my professional life . Anytime I have encountered a lack of services for a specific group of people on a particular topic , I have taken it as a hint from the Universe to expand . To grow . To create the resources that did not exist that people so desperately want and need .
 Sunstone Health & Wellness is a direct response to a 2013 report in the Indianapolis Star that ranked Indiana as the 8th most overweight state in the United States .
 How could I pretend not to see this ? To pretend as if the people of my community , my state , my country , were not struggling ? To turn a blind eye to the reality that many people are just surviving , and not thriving ?
 That ’s not my style . I want people to opt in to all things at the highest level possible .
 Something has to change . We have to change .
 My intense love for my work as a therapist , combined with my personal gravitation to all things fresh and healthy , has been the foundation for Sunstone Health & Wellness . I delight in being able to offer people innovative , cutting edge strategies for creating healthy , sustainable change .
 Sunstone Health & Wellness is focused on all aspects of health – physical , emotional , mental , and spiritual . With offices in both Greenwood and Indianapolis , we offer convenient locations regardless of where you are in the city .
 At Sunstone , we truly love people and respect the journey to living your best life possible . We want to see you grow to live a life of health and vitality . Health for your heart , mind , body , and world at Sunstone .


text:
You can download 720p & 1080p High Definition Blu-ray Quality Videos to your computer for free. Muskurahat.com have all latest hindi movie .
. Shutter Island Full Movie Download in HD Mp4 Mobile . Machina Full Movie Free Download : Caleb, a 26 year . 2 Full Movie Online, Free Download .. Hindi Artist. Latest Trailers . wapwon, wapsow, Download HD Mp4,3GP,FLV Full Mobile Videos, . monte carlo movie free downloadnaam shabana full movie online watch .
Hindi Dubbed; 720p Movies; Movie Calendar. . Kidnap Torrent Movie 2017 Free Download. . Judwaa 2 HD Movie Full 2017 Torrent Download Category: .. . free download South Indian Hindi movies 2017,Latest . Special 26 Returns (2017) hindi Dubbed full . Baahubali 2 (2017) Hindi Dubbed Full Movie HD Download .. Latest Malayalam Movies Tamil Movies Bollywood Movies Hollywood Movies Telugu Movies Kannada Movies Free Download MKV MP4 . Hindi 720p DVDRip x264 . Full Movie .

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You can download 720p & 1080p High Definition Blu - ray Quality Videos to your computer for free . Muskurahat.com have all latest hindi movie .
 . Shutter Island Full Movie Download in HD Mp4 Mobile . Machina Full Movie Free Download : Caleb , a 26 year . 2 Full Movie Online , Free Download .. Hindi Artist . Latest Trailers . wapwon , wapsow , Download HD Mp4,3GP , FLV Full Mobile Videos , . monte carlo movie free downloadnaam shabana full movie online watch .
 Hindi Dubbed ; 720p Movies ; Movie Calendar . . Kidnap Torrent Movie 2017 Free Download . . Judwaa 2 HD Movie Full 2017 Torrent Download Category : .. . free download South Indian Hindi movies 2017,Latest . Special 26 Returns ( 2017 ) hindi Dubbed full . Baahubali 2 ( 2017 ) Hindi Dubbed Full Movie HD Download .. Latest Malayalam Movies Tamil Movies Bollywood Movies Hollywood Movies Telugu Movies Kannada Movies Free Download MKV MP4 . Hindi 720p DVDRip x264 . Full Movie .


text:
Look up small business ideas in mumbai without investment in Wiktionary, the free dictionary.
small business ideas in mumbai without investmentWe’ve been determined to create a more productive conversation and shared vision for improving competitiveness through decarbonisation and energy efficiency – and to get people on all sides to understand one another and be ready to play their part. I want to express my thanks to the British Glass staff who have made this happen.” The unique bottle is also adorned by a range of eye-catching, hand designed labels. Each label is divided into three distinct parts: The first, an ordered pattern to represent ‘method', the middle panel showcases the logo and the third, a marbling pattern created to represent ‘madness’. Replacing hardwood windows with aluminium took careful consideration, but the PURe® System looks fantastic and from a performance perspective, it has considerably surpassed my expectations. In fact we now intend to replace all of the windows in the house with PURe® windows!” Commenting on the recent renovations, Ms Stephens said: “I am absolutely delighted with the work that has been carried out and Dortech’s customer service and professional installation has been second to none. This innovative, hexagonal shaped bottle has been designed for experimental super premium whiskey. These extra white flint glass bottles have been manufactured by Allied Glass. The whiskey was inspired by masters and apprentices working together at the new Midleton micro-distillery, one of the oldest and most iconic distilleries in the world. The brand combines tradition and innovation to convey a sense of fun and excitement. It uses two opposing patterns throughout the packaging. The straight lines symbolise method and order and the stunning marbled patterns reference madness and liquid experimentation.
This business reporting page lists articles associated with the title Seo.
If an emirates upgrade to business class led you here, you may wish to change the link to point directly to the intended article.

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Look up small business ideas in mumbai without investment in Wiktionary , the free dictionary .
 small business ideas in mumbai without investmentWe’ve been determined to create a more productive conversation and shared vision for improving competitiveness through decarbonisation and energy efficiency – and to get people on all sides to understand one another and be ready to play their part . I want to express my thanks to the British Glass staff who have made this happen . ” The unique bottle is also adorned by a range of eye - catching , hand designed labels . Each label is divided into three distinct parts : The first , an ordered pattern to represent ‘ method ' , the middle panel showcases the logo and the third , a marbling pattern created to represent ‘ madness ’ . Replacing hardwood windows with aluminium took careful consideration , but the PURe ® System looks fantastic and from a performance perspective , it has considerably surpassed my expectations . In fact we now intend to replace all of the windows in the house with PURe ® windows ! ” Commenting on the recent renovations , Ms Stephens said : “ I am absolutely delighted with the work that has been carried out and Dortech ’s customer service and professional installation has been second to none . This innovative , hexagonal shaped bottle has been designed for experimental super premium whiskey . These extra white flint glass bottles have been manufactured by Allied Glass . The whiskey was inspired by masters and apprentices working together at the new Midleton micro - distillery , one of the oldest and most iconic distilleries in the world . The brand combines tradition and innovation to convey a sense of fun and excitement . It uses two opposing patterns throughout the packaging . The straight lines symbolise method and order and the stunning marbled patterns reference madness and liquid experimentation .
 This business reporting page lists articles associated with the title Seo .
 If an emirates upgrade to business class led you here , you may wish to change the link to point directly to the intended article .


text:
Virelai is based in Denmark and play music from the middle age and from the Danish folk tradition. The repertoire includes dancing melodies from the south of Europe as well as old Nordic ballads and songs.
You can meet Virelai anywhere: in the streets making people dance wild; at a medieval market with the children singing along; at your school's, company's or best friend's party or as a soundtrack in a fantasy evening. You can be taken into a circle chain dance or sit down and fall into some old legend sang by Mia.

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Virelai is based in Denmark and play music from the middle age and from the Danish folk tradition . The repertoire includes dancing melodies from the south of Europe as well as old Nordic ballads and songs .
 You can meet Virelai anywhere : in the streets making people dance wild ; at a medieval market with the children singing along ; at your school 's , company 's or best friend 's party or as a soundtrack in a fantasy evening . You can be taken into a circle chain dance or sit down and fall into some old legend sang by Mia .


text: When you pick up a Shaman Bass and begin to play, you immediately know that you're holding a powerful tool. From the fine fret work and beautiful inlays, to the Grover tuners and dual pickups, this bass looks, sounds and plays great! A bass for all types of music. Get a Shaman Bass and get closer to your spiritual world.

tokenized: When you pick up a Shaman Bass and begin to play , you immediately know that you 're holding a powerful tool . From the fine fret work and beautiful inlays , to the Grover tuners and dual pickups , this bass looks , sounds and plays great ! A bass for all types of music . Get a Shaman Bass and get closer to your spiritual world .


text:
Freeze Substitution is a process for low temperature dehydration and fixation of rapidly frozen cells that usually takes days to complete. With the amazing work of K.L. McDonald and R.I. Webb1 they have now introduced a new method for freeze substitution with a basic kit that we are proud to offer.
With this unique kit researchers are now able to achieve excellent freeze substitution results in as little as 90 minutes for cells of small volume such as bacteria and tissue culture cells. For those cells of greater volume or that have significant diffusion barriers such as cuticles or thick cell walls, one can extend the time to 3 hours simply by putting a lid on the box.
Cool the metal block by submerging completely in liquid nitrogen in the ice bucket. Leave for 5 minutes or until the "boiling" stops.
In a separate box, transfer samples into cryovials with frozen fixative, keeping everything at liquid nitrogen temperatures. Seal tightly and be very sure that there is no liquid nitrogen trapped in the vial. Trapped liquid can cause the vials to explode upon warming. It is best to use a room temperature lid when sealing the tube.
Put the vials with samples into holes in the cooled metal block.
Go to a PC (Macs won't work) that has the Lascar datalogger software installed and name and start the program.
Pour off the liquid nitrogen from the block and box, making sure not to let the cryovials come out of the holes in the block.
Arrange the block so the cryovials are horizontal and put the tops of the vials against one side of the foam box. Use a piece of foam or wadded up paper behind the block so it keeps the vials from falling out of the block during shaking.
Turn on the shaker at 100-125 rpm and allow it to gradually warm until the temperature is at least 0°C before removing the vials for rinsing and resin infiltration. This operation should take place in a fume hood in case there is any leakage of osmium-acetone from poorly sealed vials. Freeze substitution will take about 2 hours with the lid off, and about 3 hours with the lid on (though this may vary from lab to lab).
Remove the vials from the metal block and place them onto a rocker at room temperature and wait until they come to about 20°C, then stop the datalogger and save the files.
Rinse out the fixative with 3-4 rinses in acetone and proceed to infiltration and embedding. Take care opening the vials as pressure built up inside can cause a spray of the freeze substitution media.
Tobacco leaf prepared by the quick FS method (Webb & McDonald, 2011). Abbreviations: N = nucleus; Ch = chloroplast; CW = cell wall; Cy = cytoplasm; ER = endoplasmic reticulum; V = vacuole; and, G = Golgi apparatus. Bar = 0.5 µm. Tobacco leaf prepared as above showing details of the cell wall (CW) and chloroplast (Ch) and cell membranes. Bar = 100 nm.
In well-frozen samples the water molecules are not likely to move around very much, even as the temperatures rise to a point where you would expect hexagonal ice (Dubochet, 2007).
Agitation should speed up the substitution of acetone for water molecules in much the same way that agitation speeds up development of film.
Water in the substitution mixture does not appear to slow down substitution as was once believed. In fact, it is known to help membrane contrast (Buser and Walther, 2008).
Does it work for all samples?
If a sample can be successfully freeze substituted by the old methods, then the quick FS method should work just as well.
McDonald and Webb have used this procedure with complete success for over a year and a half for all the samples they have freeze substituted.
If samples show evidence of ice damage then it is because they were damaged during freezing and not during freeze substitution.
Typical temperature curves using the EMS Freeze Substitution Kit. With the lid OFF the time to 0°C is about 2 hours. With the lid ON the time is about 3 hours. Results may vary depending on the particular setting of the shaker. For example, hood air flow can have a definite influence on the shape of the curves.
Cross-section through nerve cells in the head of C.elegans. A BHK cell processed in 90 minutes by the quick FS method. Abbreviations: CCP = clathrin-coated pit, Cav = caveolae, EE = early endosome, IF = intermediate filaments, LE = late endosome, M = mitochondrion, MT = microtubule. Bar = 1 µm. Modified from McDonald and Webb (2011). Mitotic spindle microtubules and spindle pole bodies in the budding yeast, Saccharomyces cerevisiae.
The equipment should be used in a fume hood in case there is a leak of osmium-acetone during a run. We suggest doing a trial run with acetone only in the cryovials to make sure that they are sealed correctly.
When sealing cryotubes that contain frozen fixative and sample, use a warm cap so that the O-ring is flexible and gives a good seal.
Take care when removing the caps after a FS run because there is some pressure built up inside the cryotubes and you can spray osmium/acetone on your hands if you are not careful. Cover the cap with a piece of lab tissue when removing and wear gloves.
Dubochet, J. (2007). The physics of rapid cooling and its implications for cryoimmobilization of cells. Meth. Cell Biol. 79, 7-21.
Buser, C., & Walther, P. (2008). Freeze substitution: the addition of water to polar solvents enhances the retention of structure and acts at temperatures around -60°C. J. Microsc. 230(2), 268-277.

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Freeze Substitution is a process for low temperature dehydration and fixation of rapidly frozen cells that usually takes days to complete . With the amazing work of K.L. McDonald and R.I. Webb1 they have now introduced a new method for freeze substitution with a basic kit that we are proud to offer .
 With this unique kit researchers are now able to achieve excellent freeze substitution results in as little as 90 minutes for cells of small volume such as bacteria and tissue culture cells . For those cells of greater volume or that have significant diffusion barriers such as cuticles or thick cell walls , one can extend the time to 3 hours simply by putting a lid on the box .
 Cool the metal block by submerging completely in liquid nitrogen in the ice bucket . Leave for 5 minutes or until the " boiling " stops .
 In a separate box , transfer samples into cryovials with frozen fixative , keeping everything at liquid nitrogen temperatures . Seal tightly and be very sure that there is no liquid nitrogen trapped in the vial . Trapped liquid can cause the vials to explode upon warming . It is best to use a room temperature lid when sealing the tube .
 Put the vials with samples into holes in the cooled metal block .
 Go to a PC ( Macs wo n't work ) that has the Lascar datalogger software installed and name and start the program .
 Pour off the liquid nitrogen from the block and box , making sure not to let the cryovials come out of the holes in the block .
 Arrange the block so the cryovials are horizontal and put the tops of the vials against one side of the foam box . Use a piece of foam or wadded up paper behind the block so it keeps the vials from falling out of the block during shaking .
 Turn on the shaker at 100 - 125 rpm and allow it to gradually warm until the temperature is at least 0 ° C before removing the vials for rinsing and resin infiltration . This operation should take place in a fume hood in case there is any leakage of osmium - acetone from poorly sealed vials . Freeze substitution will take about 2 hours with the lid off , and about 3 hours with the lid on ( though this may vary from lab to lab ) .
 Remove the vials from the metal block and place them onto a rocker at room temperature and wait until they come to about 20 ° C , then stop the datalogger and save the files .
 Rinse out the fixative with 3 - 4 rinses in acetone and proceed to infiltration and embedding . Take care opening the vials as pressure built up inside can cause a spray of the freeze substitution media .
 Tobacco leaf prepared by the quick FS method ( Webb & McDonald , 2011 ) . Abbreviations : N = nucleus ; Ch = chloroplast ; CW = cell wall ; Cy = cytoplasm ; ER = endoplasmic reticulum ; V = vacuole ; and , G = Golgi apparatus . Bar = 0.5 µm . Tobacco leaf prepared as above showing details of the cell wall ( CW ) and chloroplast ( Ch ) and cell membranes . Bar = 100 nm .
 In well - frozen samples the water molecules are not likely to move around very much , even as the temperatures rise to a point where you would expect hexagonal ice ( Dubochet , 2007 ) .
 Agitation should speed up the substitution of acetone for water molecules in much the same way that agitation speeds up development of film .
 Water in the substitution mixture does not appear to slow down substitution as was once believed . In fact , it is known to help membrane contrast ( Buser and Walther , 2008 ) .
 Does it work for all samples ?
 If a sample can be successfully freeze substituted by the old methods , then the quick FS method should work just as well .
 McDonald and Webb have used this procedure with complete success for over a year and a half for all the samples they have freeze substituted .
 If samples show evidence of ice damage then it is because they were damaged during freezing and not during freeze substitution .
 Typical temperature curves using the EMS Freeze Substitution Kit . With the lid OFF the time to 0 ° C is about 2 hours . With the lid ON the time is about 3 hours . Results may vary depending on the particular setting of the shaker . For example , hood air flow can have a definite influence on the shape of the curves .
 Cross - section through nerve cells in the head of C.elegans . A BHK cell processed in 90 minutes by the quick FS method . Abbreviations : CCP = clathrin - coated pit , Cav = caveolae , EE = early endosome , IF = intermediate filaments , LE = late endosome , M = mitochondrion , MT = microtubule . Bar = 1 µm . Modified from McDonald and Webb ( 2011 ) . Mitotic spindle microtubules and spindle pole bodies in the budding yeast , Saccharomyces cerevisiae .
 The equipment should be used in a fume hood in case there is a leak of osmium - acetone during a run . We suggest doing a trial run with acetone only in the cryovials to make sure that they are sealed correctly .
 When sealing cryotubes that contain frozen fixative and sample , use a warm cap so that the O - ring is flexible and gives a good seal .
 Take care when removing the caps after a FS run because there is some pressure built up inside the cryotubes and you can spray osmium / acetone on your hands if you are not careful . Cover the cap with a piece of lab tissue when removing and wear gloves .
 Dubochet , J. ( 2007 ) . The physics of rapid cooling and its implications for cryoimmobilization of cells . Meth . Cell Biol . 79 , 7 - 21 .
 Buser , C. , & Walther , P. ( 2008 ) . Freeze substitution : the addition of water to polar solvents enhances the retention of structure and acts at temperatures around -60 ° C . J. Microsc . 230(2 ) , 268 - 277 .


text:
To allow UCLA students to read reviews or provide feedback about apartments and landlords in Westwood.
Westwood Landlords unfairly take thousands of dollars from UCLA students every year. It's not fair, and UCLA students deserve better. Well, now we have a site to pass on the wisdom and experience from one Westwood renter to the next. Tell your friends! They'll thank you for it.
For UCLA students to find sublets or apartments to rent in Westwood.
Rather than walk around looking for phone numbers, lifeafterdorms.com makes it easy to search for the exact apartment you need. Every year people have to trudge around Westwood and find apartment phone numbers, call to see if they have 3 Bedroom apartments, etc. Well, we already did it for you! Browse all of Westwood and find the perfect apartment.
Do you have questions, comments, or suggestions? Email us!

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To allow UCLA students to read reviews or provide feedback about apartments and landlords in Westwood .
 Westwood Landlords unfairly take thousands of dollars from UCLA students every year . It 's not fair , and UCLA students deserve better . Well , now we have a site to pass on the wisdom and experience from one Westwood renter to the next . Tell your friends ! They 'll thank you for it .
 For UCLA students to find sublets or apartments to rent in Westwood .
 Rather than walk around looking for phone numbers , lifeafterdorms.com makes it easy to search for the exact apartment you need . Every year people have to trudge around Westwood and find apartment phone numbers , call to see if they have 3 Bedroom apartments , etc . Well , we already did it for you ! Browse all of Westwood and find the perfect apartment .
 Do you have questions , comments , or suggestions ? Email us !


text:
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You acknowledge that GA has no liability whatsoever for any information, representation or statement made in third party material displayed upon or accessed from this website.
The content of external Internet sites which may be reached from this website via hyperlinks or which are referred to this website is ‘third-party content’ on which GA has no influence and for which GA takes no responsibility.
You acknowledge that GA has no liability for any unlawful activity on or through this website by any third party not under or within its control, provided it has acted reasonably to prevent such activity. All such activity will be reported to the appropriate authorities.
If you consider any matter or statement in this website offensive, you should complain at first instance using the GA Complaints Procedure. If the matter is not resolved to your satisfaction you should forward the complaint to the Information Commissioner.
You agree in using this site that GA may use data technologies (known as ‘cookies’) – as do most websites- to record certain data files or pieces of information whenever you visit or connect to this website and its contents.
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Cookies are used expressly to speed up your access to website content on current and subsequent visits to the site and to make navigation around the site easier and quicker. They do not collect personal data.
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tokenized:
You acknowledge and accept that any and all information ( including but not restricted to all text , graphics , images , audio / visual files , program data , coding and other such material ) displayed upon or used within this website is subject to copyright , is owned by GA or a third party acting in concert with GA and whilst GA chooses to allow the information to be viewed by you and any other visitor to the website GA expressly forbids you from copying , extracting or in any way reproducing the content or text upon or within the website by any medium and in any forum whatsoever .
 GA provides no warranty of any kind as to the reliability of any statement or information stated upon this website ( save regarding the services it provides to registered users ) and accepts no liability whatsoever for its authenticity or accuracy .
 You acknowledge that GA has no liability whatsoever for any information , representation or statement made in third party material displayed upon or accessed from this website .
 The content of external Internet sites which may be reached from this website via hyperlinks or which are referred to this website is ‘ third - party content ’ on which GA has no influence and for which GA takes no responsibility .
 You acknowledge that GA has no liability for any unlawful activity on or through this website by any third party not under or within its control , provided it has acted reasonably to prevent such activity . All such activity will be reported to the appropriate authorities .
 If you consider any matter or statement in this website offensive , you should complain at first instance using the GA Complaints Procedure . If the matter is not resolved to your satisfaction you should forward the complaint to the Information Commissioner .
 You agree in using this site that GA may use data technologies ( known as ‘ cookies ’ ) – as do most websites- to record certain data files or pieces of information whenever you visit or connect to this website and its contents .
 These cookies help GA to provide you with a better service because they enable it to analyse the viewing trends of visitors to our sites .
 Cookies are used expressly to speed up your access to website content on current and subsequent visits to the site and to make navigation around the site easier and quicker . They do not collect personal data .
 The content of this website and all terms within it falls exclusively under UK law and is governed by the Courts of England & Wales . It is agreed that any action by GA for breach of these terms may be commenced in any relevant or applicable jurisdiction .