text:
Sci Rep. 2015 Jun 22;5:11541. doi: 10.1038/srep11541.
Voltage-gated Na+ Channel Activity Increases Colon Cancer Transcriptional Activity and Invasion Via Persistent MAPK Signaling.
House CD1, Wang BD2, Ceniccola K2, Williams R2, Simaan M3, Olender J2, Patel V3, Baptista-Hon DT4, Annunziata CM5, Gutkind JS3, Hales TG4, Lee NH2.
1] Department of Pharmacology and Physiology, The George Washington University, Washington, DC  Women's Malignancies Branch, National Cancer Institute, Bethesda, MD.
Department of Pharmacology and Physiology, The George Washington University, Washington, DC.
The National Institute of Dental and Craniofacial Research, Bethesda, MD.
The Institute of Academic Anaesthesia, Division of Neuroscience, Ninewells Hospital, University of Dundee, Dundee, DD1 9SY, UK.
Women's Malignancies Branch, National Cancer Institute, Bethesda, MD.
Functional expression of voltage-gated Na(+) channels (VGSCs) has been demonstrated in multiple cancer cell types where channel activity induces invasive activity. The signaling mechanisms by which VGSCs promote oncogenesis remain poorly understood. We explored the signal transduction process critical to VGSC-mediated invasion on the basis of reports linking channel activity to gene expression changes in excitable cells. Coincidentally, many genes transcriptionally regulated by the SCN5A isoform in colon cancer have an over-representation of cis-acting sites for transcription factors phosphorylated by ERK1/2 MAPK. We hypothesized that VGSC activity promotes MAPK activation to induce transcriptional changes in invasion-related genes. Using pharmacological inhibitors/activators and siRNA-mediated gene knockdowns, we correlated channel activity with Rap1-dependent persistent MAPK activation in the SW620 human colon cancer cell line. We further demonstrated that VGSC activity induces downstream changes in invasion-related gene expression via a PKA/ERK/c-JUN/ELK-1/ETS-1 transcriptional pathway. This is the first study illustrating a molecular mechanism linking functional activity of VGSCs to transcriptional activation of invasion-related genes.
Veratridine-enhanced Na+ current, invasion potential, and MAPK signaling.
a) Representative voltage-activated Na+ currents recorded from voltage-clamped SW620 cells in the absence and presence of 100 μM veratridine. Currents were activated by depolarizing to voltages between −70 and +20 mV in 10 mV steps. b) A graph depicting the voltage-dependence of activation and inactivation of conductance mediated by VGSCs in the absence (closed circles) or presence (open circles) of 100 μM veratridine expressed as a percentage of maximal conductance. Data were fitted with a Boltzmann function, as described previously. Values for V½ of activation and inactivation in the absence (−4.9 ± 2.7 and −86 ± 1.5 mV; n = 15, respectively) and presence of veratridine (−2.4 ± 5.3 and −95 ± 3.0 mV; n = 10, respectively) were determined as described in the Methods. The values for V½ of VGSC inactivation in the absence and presence of veratridine differed significantly (p < 0.05, unpaired t-test). The inset graph illustrates the predicted window of available current within the fits to activation and inactivation curves, in the absence (solid lines) and presence (dashed lines) of veratridine. Veratridine increases the size of the window current by reducing the extent of inactivation. c) Total number of invading SW620 cells as assessed by Matrigel assay over 24, 48 and 72 hr was significantly increased in the presence of 100 μM veratridine. Vehicle is < 0.1% DMSO final concentration. d) Western blot analysis demonstrates increase in activated ERK that occurs with short exposure to veratridine. Note that slight diminution of total ERK1/2 at 5 min is loading artifact. e) Western blot analysis demonstrates increase in activated ERK that occurs with prolonged exposure to veratridine. Traces are representative of 3 independent experiments. Columns, mean from at least 3 independent experiments; bars, SEM. * significantly different compared with vehicle control, P < 0.05, two-sided unpaired t-test (c); * significantly different compared with vehicle control (0 min), P < 0.05, ANOVA with post-hoc Dunnett’s (d); * significantly different compared to 24, 48 and 72 hr vehicle control, P < 0.05, ANOVA with post-hoc Holm test, all other pairwise comparisons non-significant (e).
Inhibition of VGSC current reduces invasion potential and MAPK signaling.
a) Lidocaine concentration-response relationship for inhibition of Na+ currents recorded from SW620 colon cancer cells. Currents were activated by stepping from a holding potential of −80 mV to 10 mV. The inhibition was measured at the peak of the current. Data, fitted by using the logistic equation to determine the IC50 value for lidocaine, are presented as mean ± SEM of 5 independent experiments for each data point. The exemplar current reveals the inhibitory effect of 10 μM lidocaine. The current is expanded to illustrate the inhibition of the peak current. b) Total number of invading SW620 cells over 24, 48 and 72 hr as assessed by Matrigel assay was significantly decreased in the presence of 100 μM lidocaine, a local anesthetic that blocks VGSCs. Vehicle is <0.1% ethanol final concentration. c) Western blot analysis demonstrates the loss of activated ERK that occurs with 72 hr lidocaine (100 μM) treatment. A representative western blot is shown (top). Results are means ± SEM from at least 5 independent experiments (a, b and c). * Significantly different compared with vehicle control, P < 0.05, two-sided unpaired t-test.
Invasion potential and MAPK signaling is mediated by Nav1.5 isoform.
a) Total number of invading cells is significantly decreased with 30 μM TTX and significantly increased in the presence of 100 μM veratridine over a 48 hr time period. Veratridine-stimulated invasion is abrogated in the presence of TTX. b) siRNA-mediated knockdown of SCN5A significantly reduced activated ERK1/2 levels compared to cells treated with a nonsense siRNA and abrogated veratridine-stimulated ERK1/2 activation. Two separate siRNAs were combined (siSCN5A) for knockdown experiments (the 2 siRNAs were previously demonstrated to lack off-target effects)4. A nonsense siRNA (siNonsense) served as control. * Significantly different for all pairwise comparisons, # significantly different for all pairwise comparisons. P < 0.05, ANOVA with post-hoc Holm test (a and b). Results are means ± SEM from at least 3–5 independent experiments for each data point.
MAPK signaling contributes to invasion potential of colon cancer cells.
a) Inhibition of MEK by either 10 μM U0126 or 25 μM PD98059 significantly reduces invasion over 48 hr. U0126 treatment abrogates both (b) veratridine-stimulated invasion and (c) MAPK activity over a 48 hr time period in SW620 cells. Results are means ± SEM from at least 4 independent experiments for each data point. * Significantly different from Vehicle Control, P < 0.05, ANOVA with post-hoc Dunnett’s (a); * significantly different for all pairwise comparisons, P < 0.05, ANOVA with post-hoc Holm test (b); * significantly different from Veratridine, P < 0.05, two-sided unpaired t-test (c).
Veratridine signals through Rap1 to enhance invasiveness and persistent MAPK signaling in colon cancer cells.
a) SW620 cells were serum starved for 24 hr prior to treatment with 100 μM veratridine for time periods indicated. A 10 min, 10 μM forskolin treatment was included as a positive control. Cell lysates were subjected to pulldown assay for detecting activated GTP-bound Rap1. An increase in GTP-bound Rap1 can be seen starting at 5 min and persists through 24 hr. b) siRNA-mediated knockdown of Rap1A and Rap1B inhibited basal SW620 invasion through Matrigel at 48 hr. Two separate siRNAs (−1 and −2) were employed for each Rap1 isotype. A nonsense siRNA (siNonsense) served as control. c) siRNA-mediated knockdown of Rap1A and Rap1B inhibited veratridine-stimulated SW620 invasion through Matrigel at 48 hr. d) siRNA-mediated knockdown of Rap1B (siRAP1B), but not Rap1A (siRAP1A), inhibited veratridine-stimulated ERK1/2 activity at 48 hr. Representative western blot provided above bar graph. In the experiments depicted in panels c and d, both siRNAs (−1 and −2) for each respective Rap1 isotype were combined for knockdown experiments, as off-target effects were not observed (see panel b). The combination of siRNAs led to greater knockdown efficiency as defined by qRT-PCR of Rap1B and Rap1B mRNAs. Results are means ± SEM from at least 4 independent experiments for each data point. * Significantly different from siNonsense control, P < 0.05, ANOVA with post-hoc Dunnett’s (b); * significantly different for all pairwise comparisons, # significantly different for all pairwise comparisons (except for siRAP1A versus siRAP1B), P < 0.05, ANOVA with post-hoc Holm test, all other pairwise comparisons non-significant (c and d).
Veratridine signals through PKA to enhance invasiveness and persistent MAPK signaling in SW620 colon cancer cells.
a) Baseline Matrigel invasion by SW620 cells over 48 hr was significantly decreased in the presence of 10 μM H89, a PKA inhibitor. b) Veratridine-stimulated (100 μM) invasion by SW620 cells at 48 hr was inhibited by pretreatment with 10 μM H89. c) Veratridine-stimulated (100 μM) invasion by SW620 cells at 48 hr is inhibited by siRNA-mediated knockdown of PKA. Two separate siRNAs targeting both the alpha and beta PKA catalytic subunit isoforms were employed in knockdown experiments (siPKAc-1 = siPKACA_1 + siPKACB_1, and siPKAc-2 = siPKACA_2 + siPKACB_2). d) Western blot analysis demonstrated an inhibition of 100 μM veratridine-stimulated ERK1/2 phosphorylation in SW620 cells at 48 hr following 10 μM pretreatment with the PKA inhibitor H89. Representative blot is shown (top). e) Veratridine-stimulated Rap1 loading of GTP is inhibited by 10 μM pretreatment with the PKA inhibitor H89. Forskolin (10 μM) served as a positive control. Representative blot is shown (top). Results are means ± SEM from at least 3–6 independent experiments for each time point in a-e. * Significantly different from vehicle control, P < 0.05, two-sided unpaired t-test (a); * significantly different for all pairwise comparisons (b-d), # significantly different for all pairwise comparisons except for siPKAc-1 versus siPKAc-2 (b and c), P < 0.05, ANOVA with post-hoc Holm test, all other pairwise comparisons non-significant (b-d); * significantly different from Vehicle Control, P < 0.05, ANOVA with post-hoc Dunnett’s (e).
Veratridine does not promote cAMP accumulation but abrogates co-localization of IkBα and PKA.
a) SW620 cells were grown to confluence in 12 well plates and serum starved for 18 hr prior to treatment with 100 μM veratridine or 10 μM forskolin for the indicated time periods. Veratridine stimulation did not induce accumulation of cAMP (as measured by RIA) compared to control (0 min). Forskolin treatment (positive control) induced a significant increase in cAMP immunoreactivity. Results are means ± SEM from at least 3 independent experiments for each time point. b) Veratridine stimulation (100 μM) reverses cytoplasmic colocalization of PKA and IKBα. Confocal immunofluorescence of SW620 cells were triple-stained for PKA catalytic subunit alpha isoform 2 (green), IkBα (red) and nuclei by DAPI (blue). PKA/IkBα colocalization shown in yellow (PKA/IkBα colocalize panels). Confocal images are representative of 6 independent experiments. c) Western blot analysis demonstrates veratridine-stimulated ERK1/2 phosphorylation in SW620 cells at 30 min correlates with IkBα degradation. Results are means ± SEM from at least 3 independent experiments, * Significantly different from 0 min control, P < 0.05, ANOVA with post-hoc Dunnett’s (a); * significantly different from Vehicle P < 0.05, two-sided unpaired t-test (c).
Activation of sodium channels induces expression changes of invasion-related genes via a PKA/ERK/c-JUN/ELK-1/ETS-1 transcriptional pathway.
a) Veratridine-stimulation of invasion-related gene expression in SW620 cells at 24 hr was reversed by pretreatment with the PKA inhibitor H89 and MEK inhibitor U0126. qRT-PCR results of invasion-related gene expression in SW620 cells treated with vehicle, 100 μM veratridine, 100 μM veratridine +10 μM H89, or 100 μM veratridine +10 μM U0126. b) Veratridine increased binding of transcription factors ETS1, c-Jun and ELK1 to invasion-related genes as shown by ChIP-PCR. Representative blots are depicted above bar graphs. ACTB (actin B) served as a negative control where veratridine stimulation did not increase transcription factor binding to the ACTB promoter compared to vehicle-treated cells. IP, immunoprecipitation of transcription factor bound to chromatin; No Ab, no antibody treatment control. c) siRNA-mediated knockdown of invasion-related genes abrogated veratridine-stimulated invasion of SW620 cells through Matrigel at 48 hr. Results are means ± SEM from at least 3–6 independent experiments for each time point in a-c. * Significantly different from corresponding vehicle control, P < 0.05, ANOVA with post-hoc Dunnett’s (a); * significantly different from corresponding vehicle control, P < 0.05, two-sided unpaired t-test (b); * significantly different for all pairwise comparisons, P < 0.05, ANOVA with post-hoc Holm test (c).

lowercased:
sci rep. 2015 jun 22;5:11541. doi: 10.1038/srep11541.
voltage-gated na+ channel activity increases colon cancer transcriptional activity and invasion via persistent mapk signaling.
house cd1, wang bd2, ceniccola k2, williams r2, simaan m3, olender j2, patel v3, baptista-hon dt4, annunziata cm5, gutkind js3, hales tg4, lee nh2.
1] department of pharmacology and physiology, the george washington university, washington, dc  women's malignancies branch, national cancer institute, bethesda, md.
department of pharmacology and physiology, the george washington university, washington, dc.
the national institute of dental and craniofacial research, bethesda, md.
the institute of academic anaesthesia, division of neuroscience, ninewells hospital, university of dundee, dundee, dd1 9sy, uk.
women's malignancies branch, national cancer institute, bethesda, md.
functional expression of voltage-gated na(+) channels (vgscs) has been demonstrated in multiple cancer cell types where channel activity induces invasive activity. the signaling mechanisms by which vgscs promote oncogenesis remain poorly understood. we explored the signal transduction process critical to vgsc-mediated invasion on the basis of reports linking channel activity to gene expression changes in excitable cells. coincidentally, many genes transcriptionally regulated by the scn5a isoform in colon cancer have an over-representation of cis-acting sites for transcription factors phosphorylated by erk1/2 mapk. we hypothesized that vgsc activity promotes mapk activation to induce transcriptional changes in invasion-related genes. using pharmacological inhibitors/activators and sirna-mediated gene knockdowns, we correlated channel activity with rap1-dependent persistent mapk activation in the sw620 human colon cancer cell line. we further demonstrated that vgsc activity induces downstream changes in invasion-related gene expression via a pka/erk/c-jun/elk-1/ets-1 transcriptional pathway. this is the first study illustrating a molecular mechanism linking functional activity of vgscs to transcriptional activation of invasion-related genes.
veratridine-enhanced na+ current, invasion potential, and mapk signaling.
a) representative voltage-activated na+ currents recorded from voltage-clamped sw620 cells in the absence and presence of 100 μm veratridine. currents were activated by depolarizing to voltages between −70 and +20 mv in 10 mv steps. b) a graph depicting the voltage-dependence of activation and inactivation of conductance mediated by vgscs in the absence (closed circles) or presence (open circles) of 100 μm veratridine expressed as a percentage of maximal conductance. data were fitted with a boltzmann function, as described previously. values for v½ of activation and inactivation in the absence (−4.9 ± 2.7 and −86 ± 1.5 mv; n = 15, respectively) and presence of veratridine (−2.4 ± 5.3 and −95 ± 3.0 mv; n = 10, respectively) were determined as described in the methods. the values for v½ of vgsc inactivation in the absence and presence of veratridine differed significantly (p < 0.05, unpaired t-test). the inset graph illustrates the predicted window of available current within the fits to activation and inactivation curves, in the absence (solid lines) and presence (dashed lines) of veratridine. veratridine increases the size of the window current by reducing the extent of inactivation. c) total number of invading sw620 cells as assessed by matrigel assay over 24, 48 and 72 hr was significantly increased in the presence of 100 μm veratridine. vehicle is < 0.1% dmso final concentration. d) western blot analysis demonstrates increase in activated erk that occurs with short exposure to veratridine. note that slight diminution of total erk1/2 at 5 min is loading artifact. e) western blot analysis demonstrates increase in activated erk that occurs with prolonged exposure to veratridine. traces are representative of 3 independent experiments. columns, mean from at least 3 independent experiments; bars, sem. * significantly different compared with vehicle control, p < 0.05, two-sided unpaired t-test (c); * significantly different compared with vehicle control (0 min), p < 0.05, anova with post-hoc dunnett’s (d); * significantly different compared to 24, 48 and 72 hr vehicle control, p < 0.05, anova with post-hoc holm test, all other pairwise comparisons non-significant (e).
inhibition of vgsc current reduces invasion potential and mapk signaling.
a) lidocaine concentration-response relationship for inhibition of na+ currents recorded from sw620 colon cancer cells. currents were activated by stepping from a holding potential of −80 mv to 10 mv. the inhibition was measured at the peak of the current. data, fitted by using the logistic equation to determine the ic50 value for lidocaine, are presented as mean ± sem of 5 independent experiments for each data point. the exemplar current reveals the inhibitory effect of 10 μm lidocaine. the current is expanded to illustrate the inhibition of the peak current. b) total number of invading sw620 cells over 24, 48 and 72 hr as assessed by matrigel assay was significantly decreased in the presence of 100 μm lidocaine, a local anesthetic that blocks vgscs. vehicle is <0.1% ethanol final concentration. c) western blot analysis demonstrates the loss of activated erk that occurs with 72 hr lidocaine (100 μm) treatment. a representative western blot is shown (top). results are means ± sem from at least 5 independent experiments (a, b and c). * significantly different compared with vehicle control, p < 0.05, two-sided unpaired t-test.
invasion potential and mapk signaling is mediated by nav1.5 isoform.
a) total number of invading cells is significantly decreased with 30 μm ttx and significantly increased in the presence of 100 μm veratridine over a 48 hr time period. veratridine-stimulated invasion is abrogated in the presence of ttx. b) sirna-mediated knockdown of scn5a significantly reduced activated erk1/2 levels compared to cells treated with a nonsense sirna and abrogated veratridine-stimulated erk1/2 activation. two separate sirnas were combined (siscn5a) for knockdown experiments (the 2 sirnas were previously demonstrated to lack off-target effects)4. a nonsense sirna (sinonsense) served as control. * significantly different for all pairwise comparisons, # significantly different for all pairwise comparisons. p < 0.05, anova with post-hoc holm test (a and b). results are means ± sem from at least 3–5 independent experiments for each data point.
mapk signaling contributes to invasion potential of colon cancer cells.
a) inhibition of mek by either 10 μm u0126 or 25 μm pd98059 significantly reduces invasion over 48 hr. u0126 treatment abrogates both (b) veratridine-stimulated invasion and (c) mapk activity over a 48 hr time period in sw620 cells. results are means ± sem from at least 4 independent experiments for each data point. * significantly different from vehicle control, p < 0.05, anova with post-hoc dunnett’s (a); * significantly different for all pairwise comparisons, p < 0.05, anova with post-hoc holm test (b); * significantly different from veratridine, p < 0.05, two-sided unpaired t-test (c).
veratridine signals through rap1 to enhance invasiveness and persistent mapk signaling in colon cancer cells.
a) sw620 cells were serum starved for 24 hr prior to treatment with 100 μm veratridine for time periods indicated. a 10 min, 10 μm forskolin treatment was included as a positive control. cell lysates were subjected to pulldown assay for detecting activated gtp-bound rap1. an increase in gtp-bound rap1 can be seen starting at 5 min and persists through 24 hr. b) sirna-mediated knockdown of rap1a and rap1b inhibited basal sw620 invasion through matrigel at 48 hr. two separate sirnas (−1 and −2) were employed for each rap1 isotype. a nonsense sirna (sinonsense) served as control. c) sirna-mediated knockdown of rap1a and rap1b inhibited veratridine-stimulated sw620 invasion through matrigel at 48 hr. d) sirna-mediated knockdown of rap1b (sirap1b), but not rap1a (sirap1a), inhibited veratridine-stimulated erk1/2 activity at 48 hr. representative western blot provided above bar graph. in the experiments depicted in panels c and d, both sirnas (−1 and −2) for each respective rap1 isotype were combined for knockdown experiments, as off-target effects were not observed (see panel b). the combination of sirnas led to greater knockdown efficiency as defined by qrt-pcr of rap1b and rap1b mrnas. results are means ± sem from at least 4 independent experiments for each data point. * significantly different from sinonsense control, p < 0.05, anova with post-hoc dunnett’s (b); * significantly different for all pairwise comparisons, # significantly different for all pairwise comparisons (except for sirap1a versus sirap1b), p < 0.05, anova with post-hoc holm test, all other pairwise comparisons non-significant (c and d).
veratridine signals through pka to enhance invasiveness and persistent mapk signaling in sw620 colon cancer cells.
a) baseline matrigel invasion by sw620 cells over 48 hr was significantly decreased in the presence of 10 μm h89, a pka inhibitor. b) veratridine-stimulated (100 μm) invasion by sw620 cells at 48 hr was inhibited by pretreatment with 10 μm h89. c) veratridine-stimulated (100 μm) invasion by sw620 cells at 48 hr is inhibited by sirna-mediated knockdown of pka. two separate sirnas targeting both the alpha and beta pka catalytic subunit isoforms were employed in knockdown experiments (sipkac-1 = sipkaca_1 + sipkacb_1, and sipkac-2 = sipkaca_2 + sipkacb_2). d) western blot analysis demonstrated an inhibition of 100 μm veratridine-stimulated erk1/2 phosphorylation in sw620 cells at 48 hr following 10 μm pretreatment with the pka inhibitor h89. representative blot is shown (top). e) veratridine-stimulated rap1 loading of gtp is inhibited by 10 μm pretreatment with the pka inhibitor h89. forskolin (10 μm) served as a positive control. representative blot is shown (top). results are means ± sem from at least 3–6 independent experiments for each time point in a-e. * significantly different from vehicle control, p < 0.05, two-sided unpaired t-test (a); * significantly different for all pairwise comparisons (b-d), # significantly different for all pairwise comparisons except for sipkac-1 versus sipkac-2 (b and c), p < 0.05, anova with post-hoc holm test, all other pairwise comparisons non-significant (b-d); * significantly different from vehicle control, p < 0.05, anova with post-hoc dunnett’s (e).
veratridine does not promote camp accumulation but abrogates co-localization of ikbα and pka.
a) sw620 cells were grown to confluence in 12 well plates and serum starved for 18 hr prior to treatment with 100 μm veratridine or 10 μm forskolin for the indicated time periods. veratridine stimulation did not induce accumulation of camp (as measured by ria) compared to control (0 min). forskolin treatment (positive control) induced a significant increase in camp immunoreactivity. results are means ± sem from at least 3 independent experiments for each time point. b) veratridine stimulation (100 μm) reverses cytoplasmic colocalization of pka and ikbα. confocal immunofluorescence of sw620 cells were triple-stained for pka catalytic subunit alpha isoform 2 (green), ikbα (red) and nuclei by dapi (blue). pka/ikbα colocalization shown in yellow (pka/ikbα colocalize panels). confocal images are representative of 6 independent experiments. c) western blot analysis demonstrates veratridine-stimulated erk1/2 phosphorylation in sw620 cells at 30 min correlates with ikbα degradation. results are means ± sem from at least 3 independent experiments, * significantly different from 0 min control, p < 0.05, anova with post-hoc dunnett’s (a); * significantly different from vehicle p < 0.05, two-sided unpaired t-test (c).
activation of sodium channels induces expression changes of invasion-related genes via a pka/erk/c-jun/elk-1/ets-1 transcriptional pathway.
a) veratridine-stimulation of invasion-related gene expression in sw620 cells at 24 hr was reversed by pretreatment with the pka inhibitor h89 and mek inhibitor u0126. qrt-pcr results of invasion-related gene expression in sw620 cells treated with vehicle, 100 μm veratridine, 100 μm veratridine +10 μm h89, or 100 μm veratridine +10 μm u0126. b) veratridine increased binding of transcription factors ets1, c-jun and elk1 to invasion-related genes as shown by chip-pcr. representative blots are depicted above bar graphs. actb (actin b) served as a negative control where veratridine stimulation did not increase transcription factor binding to the actb promoter compared to vehicle-treated cells. ip, immunoprecipitation of transcription factor bound to chromatin; no ab, no antibody treatment control. c) sirna-mediated knockdown of invasion-related genes abrogated veratridine-stimulated invasion of sw620 cells through matrigel at 48 hr. results are means ± sem from at least 3–6 independent experiments for each time point in a-c. * significantly different from corresponding vehicle control, p < 0.05, anova with post-hoc dunnett’s (a); * significantly different from corresponding vehicle control, p < 0.05, two-sided unpaired t-test (b); * significantly different for all pairwise comparisons, p < 0.05, anova with post-hoc holm test (c).


text:
My Sunday morning indulgence of fully caffeinated coffee complete with real cream and real sugar ☕ Unfortunately it is partnered with some persistent swelling in my ankle. Ice, compression sock, and elevation are the name of the game. And likely a trek to the doctor for an ultrasound to rule out a blood clot.
Swelling of the feet and legs post Taxol is very common, and I noticed it beginning shortly after my last treatment. Up until this week, I’ve always been able to control it, but now it has stopped playing the game my way.
I feel as though, once again, I come to a point where I am ready to embrace my wonderful busy life, and my body says “nope, slow down, go easy, rest, and recover.” And if there is one incredibly hard lesson I have learned on this journey, it is to listen to what my body is saying – especially when it says “stop”. However, aside from this swelling and some still incredibly dry skin, most of my side effect symptoms have subsided. I am seeing hair and nail growth and health. My eyelashes have joined the party and my eyebrows are ready for some shaping.
Also in the good news department, exchange surgery is scheduled! I’m getting rid of these expanders and getting squishier (a very relative term) implants for my birthday!

lowercased:
my sunday morning indulgence of fully caffeinated coffee complete with real cream and real sugar ☕ unfortunately it is partnered with some persistent swelling in my ankle. ice, compression sock, and elevation are the name of the game. and likely a trek to the doctor for an ultrasound to rule out a blood clot.
swelling of the feet and legs post taxol is very common, and i noticed it beginning shortly after my last treatment. up until this week, i’ve always been able to control it, but now it has stopped playing the game my way.
i feel as though, once again, i come to a point where i am ready to embrace my wonderful busy life, and my body says “nope, slow down, go easy, rest, and recover.” and if there is one incredibly hard lesson i have learned on this journey, it is to listen to what my body is saying – especially when it says “stop”. however, aside from this swelling and some still incredibly dry skin, most of my side effect symptoms have subsided. i am seeing hair and nail growth and health. my eyelashes have joined the party and my eyebrows are ready for some shaping.
also in the good news department, exchange surgery is scheduled! i’m getting rid of these expanders and getting squishier (a very relative term) implants for my birthday!


text:
After decades of political instability, Nicaragua entered into a period of economic growth, decentralized governance, and poverty reduction in the 2000s. However, in 2011 President Ortega defied the country’s constitution by remaining President for a third term. Municipal elections in 2012 were also marked by irregularities, though popular support for government administration remains high (NDI, 2013).
There is no ministry at the national level specifically mandated to perform decentralization. The Nicaraguan Institute for Municipal Strengthening (INIFOM) and the Office at the Technical Secretariat of the Presidency (OAP-SETEC) play a leading role (Development Partners, 2007).
Nicaragua is divided into15 Departments and 2 autonomous regions: the North Atlantic Autonomous Region (RAAN) and the Autonomous Region of the Southern Atlantic (RAAS).
Within the departments are 111 municipalities, each with a municipal council directly elected by the citizens.
Hagamos Democracia (Let Democracy) promotes awareness and utilization of democratic values and initiatives in Nicaragua. Using an educational model, they aim to increase the direct participation of citizens and ensure the democratic processes of local authorities.
Red Nicaragüense por la Democracia y el Desarrollo Local (The Nicaraguan Network for Local Democracy and Development) provides a space for communication between members of civil society committed to local democracy.
Group FUNDEMOS is committed to facing the challenges of a democratic transition, and they strive to strengthen local democracy in Nicaragua municipalities through the development of an active civil society.
Grupo ProJusticia (Group For Justice) promotes Nicaraguans’ understanding of the constitution and their rights established within the document regarding freedom of expression.
The Institute of Strategic Studies and Public Policy (IEEPP) supports citizen participation in public affairs and aims to promote good governance. IEEPP works in both the civil society and state level to make decisions that work for both parties.
The Nicaraguan Institute of Humanistic Studies (INEH) views development with a gender perspective; therefore, their work training and strengthening democratic authorities focuses on gender equality and overall autonomy.
The Nicaraguan Institute of Municipal Development (INIFOM) represents the interests of the municipalities as well as the federal government to strike a balance between the two and move decentralization forward.
The Association of Municipalities of Nicaragua (AMUNIC) represents local governments statewide and played a key role in moving decentralization policies forward in the 1990s.
The Federation of Central American (FEMICA) is a regional non-profit that assists with capacity building workshops for municipal leaders and other local authorities. FEMICA works heavily in Nicaragua, combining efforts with local capacity building organizations, such as AMUNIC, to promote autonomous local governance.
Local authorities must seek permission from the central government to introduce new taxes, and local taxes make up nearly half of the revenue on the local level (UCLG, 2010).
The Law of Transfers (Ley de Tranferencias) came into effect in 2004 and mandates that 4 percent of state income be transferred to local municipalities. The law states that each year, the transfers will increase by one percent until they reached 10 percent in 2010 (UCLG, 2008).
The 1997 Law on Municipalities mandates participatory budgeting practices and requires that mayors hold town meetings to make the local budgets public knowledge (ICMA, 2004).
The 2003 Citizen Participation Law promotes participation through local departments and groups within municipalities (Hansen, 2007).
In 2006, the government initiated the National Policy of Decentralization, laying out the steps necessary to implement decentralization in the country (UCLG, 2008).
According to UCLG, the sewage, lighting, and solid waste sectors of government are all decentralized (UCLG, 2008).
There is no democratic representation below the level of the municipalities, so popular elections do not take place on the most local scale (UCLG, 2008).
The sectors of local government considered to remain the most centralized are public safety, education and health (UCLG, 2008).

lowercased:
after decades of political instability, nicaragua entered into a period of economic growth, decentralized governance, and poverty reduction in the 2000s. however, in 2011 president ortega defied the country’s constitution by remaining president for a third term. municipal elections in 2012 were also marked by irregularities, though popular support for government administration remains high (ndi, 2013).
there is no ministry at the national level specifically mandated to perform decentralization. the nicaraguan institute for municipal strengthening (inifom) and the office at the technical secretariat of the presidency (oap-setec) play a leading role (development partners, 2007).
nicaragua is divided into15 departments and 2 autonomous regions: the north atlantic autonomous region (raan) and the autonomous region of the southern atlantic (raas).
within the departments are 111 municipalities, each with a municipal council directly elected by the citizens.
hagamos democracia (let democracy) promotes awareness and utilization of democratic values and initiatives in nicaragua. using an educational model, they aim to increase the direct participation of citizens and ensure the democratic processes of local authorities.
red nicaragüense por la democracia y el desarrollo local (the nicaraguan network for local democracy and development) provides a space for communication between members of civil society committed to local democracy.
group fundemos is committed to facing the challenges of a democratic transition, and they strive to strengthen local democracy in nicaragua municipalities through the development of an active civil society.
grupo projusticia (group for justice) promotes nicaraguans’ understanding of the constitution and their rights established within the document regarding freedom of expression.
the institute of strategic studies and public policy (ieepp) supports citizen participation in public affairs and aims to promote good governance. ieepp works in both the civil society and state level to make decisions that work for both parties.
the nicaraguan institute of humanistic studies (ineh) views development with a gender perspective; therefore, their work training and strengthening democratic authorities focuses on gender equality and overall autonomy.
the nicaraguan institute of municipal development (inifom) represents the interests of the municipalities as well as the federal government to strike a balance between the two and move decentralization forward.
the association of municipalities of nicaragua (amunic) represents local governments statewide and played a key role in moving decentralization policies forward in the 1990s.
the federation of central american (femica) is a regional non-profit that assists with capacity building workshops for municipal leaders and other local authorities. femica works heavily in nicaragua, combining efforts with local capacity building organizations, such as amunic, to promote autonomous local governance.
local authorities must seek permission from the central government to introduce new taxes, and local taxes make up nearly half of the revenue on the local level (uclg, 2010).
the law of transfers (ley de tranferencias) came into effect in 2004 and mandates that 4 percent of state income be transferred to local municipalities. the law states that each year, the transfers will increase by one percent until they reached 10 percent in 2010 (uclg, 2008).
the 1997 law on municipalities mandates participatory budgeting practices and requires that mayors hold town meetings to make the local budgets public knowledge (icma, 2004).
the 2003 citizen participation law promotes participation through local departments and groups within municipalities (hansen, 2007).
in 2006, the government initiated the national policy of decentralization, laying out the steps necessary to implement decentralization in the country (uclg, 2008).
according to uclg, the sewage, lighting, and solid waste sectors of government are all decentralized (uclg, 2008).
there is no democratic representation below the level of the municipalities, so popular elections do not take place on the most local scale (uclg, 2008).
the sectors of local government considered to remain the most centralized are public safety, education and health (uclg, 2008).


text:
This APK is signed by burton999 and upgrades your existing app.
1.Supported the icon pack. You can change an icon according to the theme with supported launchers.
1.Improved the restore feature, you can restore any text file.
1.Translated CalcNote into Spanish, Portuguese, German, French, Italian, Russian, Korean and Indonesian.
CalcNote is a new generation of calculator designed for the smart phone.
Just type a expression, the answer will be displayed instantly. No need to tap equals.
It works like notepad or your word processor, you can see multiple questions and answers all at once.
It's like a spreadsheet but much easier and simpler.
If you make a mistake, you don't need to start again, you just fix the wrong expression.
CalcNote will instantly evaluate any mathematical expression and display result in real-time.
With a Notepad-style interface you write operations on each line and see results instantly.
You can combine text-lines with calculations.
Every line in CalcNote is evaluated so you can write different operations on each.
It even allows referencing lines as in Spreadsheet so you can do multi-line combined calculations (i.e. $2+$3 adds up the results from line 2 and 3).
Swipe right-left to switch between different keypads with multiple operations you can perform.
Regular mathematical operations, logarithmic, trigonometric functions (i.e. sin, cos), permutations/combinations as well as a percentage calculator and unit converter.
In CalcNote you can organize your calculation sheets, save them individually and even export expressions if you want to use them elsewhere (i.e. in Spreadsheet, in a scientific paper, etc.).
You can customize CalcNote by changing its background/text color, showing/hiding line numbers, fonts, display format, customize the keypad layout and much more.
This is a free version of CalcNote, which displays ad at the top of the screen.
If you'd like to remove ads, you can upgrade to Pro.
burton999 makes no warranty as to the accuracy or reliability or suitability of any calculation results or information provided through the CalcNote. burton999 is not also responsible for any damages, direct or indirect, which may occur by the calculation results or information provided through the CalcNote.
I hope this app will be helpful for you.

lowercased:
this apk is signed by burton999 and upgrades your existing app.
1.supported the icon pack. you can change an icon according to the theme with supported launchers.
1.improved the restore feature, you can restore any text file.
1.translated calcnote into spanish, portuguese, german, french, italian, russian, korean and indonesian.
calcnote is a new generation of calculator designed for the smart phone.
just type a expression, the answer will be displayed instantly. no need to tap equals.
it works like notepad or your word processor, you can see multiple questions and answers all at once.
it's like a spreadsheet but much easier and simpler.
if you make a mistake, you don't need to start again, you just fix the wrong expression.
calcnote will instantly evaluate any mathematical expression and display result in real-time.
with a notepad-style interface you write operations on each line and see results instantly.
you can combine text-lines with calculations.
every line in calcnote is evaluated so you can write different operations on each.
it even allows referencing lines as in spreadsheet so you can do multi-line combined calculations (i.e. $2+$3 adds up the results from line 2 and 3).
swipe right-left to switch between different keypads with multiple operations you can perform.
regular mathematical operations, logarithmic, trigonometric functions (i.e. sin, cos), permutations/combinations as well as a percentage calculator and unit converter.
in calcnote you can organize your calculation sheets, save them individually and even export expressions if you want to use them elsewhere (i.e. in spreadsheet, in a scientific paper, etc.).
you can customize calcnote by changing its background/text color, showing/hiding line numbers, fonts, display format, customize the keypad layout and much more.
this is a free version of calcnote, which displays ad at the top of the screen.
if you'd like to remove ads, you can upgrade to pro.
burton999 makes no warranty as to the accuracy or reliability or suitability of any calculation results or information provided through the calcnote. burton999 is not also responsible for any damages, direct or indirect, which may occur by the calculation results or information provided through the calcnote.
i hope this app will be helpful for you.


text:
Our auction is usually the 3rd Saturday of October EVERY year at the Halifax Plantation Clubhouse. This year was a huge success. Tammy was our 2018 Auction Chairwoman and she did a fabulous job of working herself, and tying everyone else who worked on the auction together. The result was a flawless and success auction.
Tammy did a wonderful job of chairing the auction. She collected most of the gift cards and big ticket prizes for the annual raffle. Kudos Tammy...Great success!
Lee is our auctioneer every year. She has an uncle who is a professional auctioneer, so it's in her blood. She cashed in her points with her bank and got several gift cards for the raffle. Great job Lee!
Joanne is the grand prize winner of the raffle. Nearly a thousand dollars worth of gift cards and many hand painted ornaments are on that gift tree. This is the 2nd time she has won the 2-night stay at a hotel and a ride around the Daytona Race Track with a professional driver. AND it's the second time she has given it to a friend who had that "wish" on his Bucket List. Joanne has won the grand prize three times. And because she's such a wonderful, generous, beautiful lady...EVERYONE was happy for her. Win it again next year Joanne...You deserve it!
Doreen, second from left has been our President for the past two years. You've been an excellent Prez Doreen.
Nedra on the far left will take over Doreen's job in January 2019. We are so lucky to have these take-charge (in a friendly and effective way) ladies.
This beautiful chair was painted by a group of Palm Coast Decorative Painters for the auction.

lowercased:
our auction is usually the 3rd saturday of october every year at the halifax plantation clubhouse. this year was a huge success. tammy was our 2018 auction chairwoman and she did a fabulous job of working herself, and tying everyone else who worked on the auction together. the result was a flawless and success auction.
tammy did a wonderful job of chairing the auction. she collected most of the gift cards and big ticket prizes for the annual raffle. kudos tammy...great success!
lee is our auctioneer every year. she has an uncle who is a professional auctioneer, so it's in her blood. she cashed in her points with her bank and got several gift cards for the raffle. great job lee!
joanne is the grand prize winner of the raffle. nearly a thousand dollars worth of gift cards and many hand painted ornaments are on that gift tree. this is the 2nd time she has won the 2-night stay at a hotel and a ride around the daytona race track with a professional driver. and it's the second time she has given it to a friend who had that "wish" on his bucket list. joanne has won the grand prize three times. and because she's such a wonderful, generous, beautiful lady...everyone was happy for her. win it again next year joanne...you deserve it!
doreen, second from left has been our president for the past two years. you've been an excellent prez doreen.
nedra on the far left will take over doreen's job in january 2019. we are so lucky to have these take-charge (in a friendly and effective way) ladies.
this beautiful chair was painted by a group of palm coast decorative painters for the auction.


text:
Pioneering singer/songwriter Joni Mitchell will join Fleetwood Mac diva Stevie Nicks and hot country singer Shania Twain for Woodstock '98, according to Mitchell's management firm, Sam Feldman Management. The lineup for the Aug. 14-16 music festival, to be held on the famed Woodstock land in Sullivan County, N.Y., is still being coordinated. The event is being presented by cable TV mogul Allen Gerry. Look for more artists to be announced.
This article has been viewed 5,731 times since being added on January 9, 2000.

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pioneering singer/songwriter joni mitchell will join fleetwood mac diva stevie nicks and hot country singer shania twain for woodstock '98, according to mitchell's management firm, sam feldman management. the lineup for the aug. 14-16 music festival, to be held on the famed woodstock land in sullivan county, n.y., is still being coordinated. the event is being presented by cable tv mogul allen gerry. look for more artists to be announced.
this article has been viewed 5,731 times since being added on january 9, 2000.


text:
Al’s Bar will be closed on Christmas Eve and Christmas Day.
The Beer Trappe on Euclid will be open Christmas Eve at normal hours but closed on Christmas Day.
Blue Stallion Brewing will be open on Christmas Eve at normal hours but will close on Christmas Day.
Buddha Lounge will be open regular hours on Christmas Eve but will be closed Christmas Day.
Cheapside Bar and Grill is open Christmas Eve but closed Christmas Day.
Chevy Chase Inn at 833 E. Euclid Avenue will be open limited hours on Christmas Eve and will be closed on Christmas Day.
Cocktails will be closed on Christmas Eve but will open at 7 pm on Christmas Day.
Country Boy Brewing at 436 Chair Avenue will be open 4-12 pm on Christmas Eve and 3-10 pm Christmas Day.
Ethereal Brewing in the Distillery District is closed for Christmas Eve and is closed for Christmas Day.
McCarthy’s Irish Bar at 117 S. Upper will be open normal hours on Christmas Eve and Christmas Day.
JDI at 319 Cedar Street will be closed Christmas Eve and will be closed Christmas Day.
Lynagh’s at 384 Woodland Avenue will be closed Christmas Eve but will open Christmas Night for drinks.
The Tin Roof on Limestone will be closed on Christmas Eve and will be closed Christmas Day.
The Upstart Crow will be open Christmas Eve but will be closed Christmas Day.
West Sixth will be open on Christmas Eve for abbreviated hours but will be closed Christmas Day.

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al’s bar will be closed on christmas eve and christmas day.
the beer trappe on euclid will be open christmas eve at normal hours but closed on christmas day.
blue stallion brewing will be open on christmas eve at normal hours but will close on christmas day.
buddha lounge will be open regular hours on christmas eve but will be closed christmas day.
cheapside bar and grill is open christmas eve but closed christmas day.
chevy chase inn at 833 e. euclid avenue will be open limited hours on christmas eve and will be closed on christmas day.
cocktails will be closed on christmas eve but will open at 7 pm on christmas day.
country boy brewing at 436 chair avenue will be open 4-12 pm on christmas eve and 3-10 pm christmas day.
ethereal brewing in the distillery district is closed for christmas eve and is closed for christmas day.
mccarthy’s irish bar at 117 s. upper will be open normal hours on christmas eve and christmas day.
jdi at 319 cedar street will be closed christmas eve and will be closed christmas day.
lynagh’s at 384 woodland avenue will be closed christmas eve but will open christmas night for drinks.
the tin roof on limestone will be closed on christmas eve and will be closed christmas day.
the upstart crow will be open christmas eve but will be closed christmas day.
west sixth will be open on christmas eve for abbreviated hours but will be closed christmas day.


text:
This visa is provided for new born babies who are less than 3 months upon their arrival along with his/her mother at the entry border.
The baby should have a passport or his/her name should be added in the passport of mother or father.
Fine for overstay: QR 10 per day of delay after expiring the specified period which is two months outside the country and three months inside country.

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this visa is provided for new born babies who are less than 3 months upon their arrival along with his/her mother at the entry border.
the baby should have a passport or his/her name should be added in the passport of mother or father.
fine for overstay: qr 10 per day of delay after expiring the specified period which is two months outside the country and three months inside country.


text:
If you have gotten your copy of The Sunday Times last week, you might have come across some familiar faces in the Scoot Supplement that came with it as well.
Yes, that's us - showcasing the best that Sydney has to offer for families with young tots. So if you are planning for a holiday in Sydney and its surrounds, do click on the picture above to read what I have to share!
I chanced upon your blog and I love reading it. I used to expect my kids to smile at the camera and feel unhappy when they dont. When i saw those natural shots u took of ur kids, be it they are moody, making funny faces or crying, it amuse me. Ur blog reminded me its just part of them growing up and to treasure every moment. I hope im not too late with my boy (4yrs) and gal(2yrs). Thanks to u, Im motivated to bring them out more often. Thanks.
Thanks for dropping by and for your extremely kind words too!

lowercased:
if you have gotten your copy of the sunday times last week, you might have come across some familiar faces in the scoot supplement that came with it as well.
yes, that's us - showcasing the best that sydney has to offer for families with young tots. so if you are planning for a holiday in sydney and its surrounds, do click on the picture above to read what i have to share!
i chanced upon your blog and i love reading it. i used to expect my kids to smile at the camera and feel unhappy when they dont. when i saw those natural shots u took of ur kids, be it they are moody, making funny faces or crying, it amuse me. ur blog reminded me its just part of them growing up and to treasure every moment. i hope im not too late with my boy (4yrs) and gal(2yrs). thanks to u, im motivated to bring them out more often. thanks.
thanks for dropping by and for your extremely kind words too!


text:
Umehara is in the throws of grief over the passing of his girlfriend. We join him as he is being consoled by his good friend, Miyagawa, who offers a hand to help. Miyagawa invites his friend to a gathering that he has been regularly attending, but it is a gathering like no other.
On arriving, Umehara finds a room full of men on their knees all looking towards an empty chair on the room’s opposite wall. The whole place has the air of a cult, with those suspicions being solidified once the target of these men’s attention appears. It is Tomie. All of the men go wild and Miyagawa reveals that he has brought his friend as a gift to Tomie in order to receive a reward.
Tomie seems taken by Umehara immediately, however, he couldn’t care less about her. It seems his love for his recently passed lady are just too strong. As the story moves forward, Tomie tries to work out why she has no effect on him. Her failure leads to her turning her worshippers against him in response. But what is truly shocking, is when the inevitable happens and the crowds of men move past the infatuation stage, and into the “I just want to kill her and cut her up” stage. By the end of this gathering, things get rather messy – and perhaps not for the reasons you may first think.
This story is about Tomie’s power of obsession over men, except it’s turned up to eleven. She essentially has an army at her disposal, and chooses to have them shower her with complements and gifts. She will demand for them to make her laugh; to entertain her in any way she shes fit. But when she doesn’t get her way, as is the case with Umehara, you’d better not be in her path.
The power that she has over these men is potent. And the conclusion of such an odd situation – with these men all sitting at her feet in a growing internal frenzy – could only lead to bad things. The whole chapter feels like a boiling pot of water just waiting to break over the sides. The final panels present a suitably violent scene for such a dangerous, high-pressure atmosphere.
Not a favourite of mine in the Tomie Collection, but still a very worthwhile addition to its overall world. Here, Junji Ito is focusing in on the side of Tomie that drives men to obsess over her – and to do anything she asks in order to please her. Although it isn’t very large in scope, when it is digested along with all of the other chapters, really give a complete picture of this Queen of horror manga.
I don’t think this would be the best chapter to serve as an introduction to the lady. Although most of what you see in here would have been seen before in earlier chapters, there is one part that I believe is new. She manages to force herself into the reoccurring dreams of Umehara. Whether it is her doing or just from the effect she’s had on him, is not clear. So perhaps she manages to win him over after all?
If you are an existing fan of the series and want to see more of what you have come to enjoy from Tomie, then Gathering will make a fine addition to that repertoire.

lowercased:
umehara is in the throws of grief over the passing of his girlfriend. we join him as he is being consoled by his good friend, miyagawa, who offers a hand to help. miyagawa invites his friend to a gathering that he has been regularly attending, but it is a gathering like no other.
on arriving, umehara finds a room full of men on their knees all looking towards an empty chair on the room’s opposite wall. the whole place has the air of a cult, with those suspicions being solidified once the target of these men’s attention appears. it is tomie. all of the men go wild and miyagawa reveals that he has brought his friend as a gift to tomie in order to receive a reward.
tomie seems taken by umehara immediately, however, he couldn’t care less about her. it seems his love for his recently passed lady are just too strong. as the story moves forward, tomie tries to work out why she has no effect on him. her failure leads to her turning her worshippers against him in response. but what is truly shocking, is when the inevitable happens and the crowds of men move past the infatuation stage, and into the “i just want to kill her and cut her up” stage. by the end of this gathering, things get rather messy – and perhaps not for the reasons you may first think.
this story is about tomie’s power of obsession over men, except it’s turned up to eleven. she essentially has an army at her disposal, and chooses to have them shower her with complements and gifts. she will demand for them to make her laugh; to entertain her in any way she shes fit. but when she doesn’t get her way, as is the case with umehara, you’d better not be in her path.
the power that she has over these men is potent. and the conclusion of such an odd situation – with these men all sitting at her feet in a growing internal frenzy – could only lead to bad things. the whole chapter feels like a boiling pot of water just waiting to break over the sides. the final panels present a suitably violent scene for such a dangerous, high-pressure atmosphere.
not a favourite of mine in the tomie collection, but still a very worthwhile addition to its overall world. here, junji ito is focusing in on the side of tomie that drives men to obsess over her – and to do anything she asks in order to please her. although it isn’t very large in scope, when it is digested along with all of the other chapters, really give a complete picture of this queen of horror manga.
i don’t think this would be the best chapter to serve as an introduction to the lady. although most of what you see in here would have been seen before in earlier chapters, there is one part that i believe is new. she manages to force herself into the reoccurring dreams of umehara. whether it is her doing or just from the effect she’s had on him, is not clear. so perhaps she manages to win him over after all?
if you are an existing fan of the series and want to see more of what you have come to enjoy from tomie, then gathering will make a fine addition to that repertoire.


text:
COLUMBIA, SC (WIS) - Lawmakers met to discuss medical marijuana in the Palmetto State on Tuesday.
The “Compassionate Care Act” would allow doctors to legally prescribe medical marijuana for some chronic conditions. Sen. Tom Davis (R-Beaufort) and Rep. Peter McCoy (R-Charleston) spoke about their medical marijuana bills at a press conference after it was introduced on the State Senate floor.
Sen. Davis said this is the most socially conservative medical marijuana bill in the country. He doesn’t want it to lay the foundation for recreation use in the state. “Each step of the process – from the growing to the processing to the dispensing is monitored in real time constantly by DHEC and SLED,” Sen. Davis said.
Sen. Davis shared some details on the Compassionate Care Act while surrounded by members of both parties in the House and Senate.
The Compassionate Care Act is proposing the use of medical cannabis for people with certain debilitating medical conditions. Those include: cancer, multiple sclerosis, a neurological disease, PTSD, glaucoma, Crohn’s disease, sickle cell anemia, ulcerative colitis, wasting syndrome, severe nausea in a person who is in hospice care, chronic medical condition causing severe muscle spasms, including multiple sclerosis: or “chronic or debilitating diseases for which an opioid is currently or could be prescribed by a physician based on generally accepted standards of care.
Some other provisions say certifying physicians must complete training that is specific to medical cannabis.
Another provision said smoking of cannabis is not allowed. It may only be used by patients in the forms such as vaporized flower or oil, gel caps, suppositories, patches, edibles or topical cream.
Sen. Davis said the medical cannabis will be tested by an independent testing lab to make sure it is safe and properly labelled.
Supporters say a benchmark research poll shows 72 percent of South Carolinians support medical marijuana. Others argue against it saying CBD oil is already legal in the state so there’s no need for medical marijuana.
Two days after a New York Times report uncovered several issues with production at the North Charleston Boeing facility, one man quoted spoke on camera with CBS News.

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columbia, sc (wis) - lawmakers met to discuss medical marijuana in the palmetto state on tuesday.
the “compassionate care act” would allow doctors to legally prescribe medical marijuana for some chronic conditions. sen. tom davis (r-beaufort) and rep. peter mccoy (r-charleston) spoke about their medical marijuana bills at a press conference after it was introduced on the state senate floor.
sen. davis said this is the most socially conservative medical marijuana bill in the country. he doesn’t want it to lay the foundation for recreation use in the state. “each step of the process – from the growing to the processing to the dispensing is monitored in real time constantly by dhec and sled,” sen. davis said.
sen. davis shared some details on the compassionate care act while surrounded by members of both parties in the house and senate.
the compassionate care act is proposing the use of medical cannabis for people with certain debilitating medical conditions. those include: cancer, multiple sclerosis, a neurological disease, ptsd, glaucoma, crohn’s disease, sickle cell anemia, ulcerative colitis, wasting syndrome, severe nausea in a person who is in hospice care, chronic medical condition causing severe muscle spasms, including multiple sclerosis: or “chronic or debilitating diseases for which an opioid is currently or could be prescribed by a physician based on generally accepted standards of care.
some other provisions say certifying physicians must complete training that is specific to medical cannabis.
another provision said smoking of cannabis is not allowed. it may only be used by patients in the forms such as vaporized flower or oil, gel caps, suppositories, patches, edibles or topical cream.
sen. davis said the medical cannabis will be tested by an independent testing lab to make sure it is safe and properly labelled.
supporters say a benchmark research poll shows 72 percent of south carolinians support medical marijuana. others argue against it saying cbd oil is already legal in the state so there’s no need for medical marijuana.
two days after a new york times report uncovered several issues with production at the north charleston boeing facility, one man quoted spoke on camera with cbs news.


text:
Airtrain stations are located outside the Brisbane Airport terminals.
Customers are saying they "Should have used it years ago"
Airtrain has a station directly outside both the Domestic and International airport terminals. Simply follow the “trains” signs from the arrivals areas in both terminals. The Domestic Terminal is approximately 2 km from the International Terminal and takes only two minutes by train.
Airtrain is located on the elevated Skywalk and is easily accessed using travellators directly in front of the domestic airport. Airtrain has information counter where you can purchase a ticket in both the Qantas and Virgin Australia arrivals areas. Our friendly staff will assist you get to your destination quickly.
Airtrain is located on level 3 on the International Terminal. The arrivals are on level 2. Simply follow the “trains” signs to level 3 using the travellators or lifts. Airtrain has an information counter where you can purchase tickets in the arrivals hall on level 2. Our friendly staff will assist you get to your destination quickly.
Trolleys are available inside the terminals and you can use these on the way to the Airtrain stations. Trolleys are not able to be taken into the station platforms or trains for safety reasons. Ask our staff if you need assistance with your bags.
Please note that for security reasons, Airtrain stations do not have luggage storage facilities. Brisbane Airport have luggage storage facilities available.

lowercased:
airtrain stations are located outside the brisbane airport terminals.
customers are saying they "should have used it years ago"
airtrain has a station directly outside both the domestic and international airport terminals. simply follow the “trains” signs from the arrivals areas in both terminals. the domestic terminal is approximately 2 km from the international terminal and takes only two minutes by train.
airtrain is located on the elevated skywalk and is easily accessed using travellators directly in front of the domestic airport. airtrain has information counter where you can purchase a ticket in both the qantas and virgin australia arrivals areas. our friendly staff will assist you get to your destination quickly.
airtrain is located on level 3 on the international terminal. the arrivals are on level 2. simply follow the “trains” signs to level 3 using the travellators or lifts. airtrain has an information counter where you can purchase tickets in the arrivals hall on level 2. our friendly staff will assist you get to your destination quickly.
trolleys are available inside the terminals and you can use these on the way to the airtrain stations. trolleys are not able to be taken into the station platforms or trains for safety reasons. ask our staff if you need assistance with your bags.
please note that for security reasons, airtrain stations do not have luggage storage facilities. brisbane airport have luggage storage facilities available.


text:
Pity the person who invests heavily in canals right before the railways start operating. You could understand, for example, why the sponsor of the Bridgewater Canal (perhaps England's first) would vehemently oppose the planned Liverpool and Manchester Railway. But the march of technology could not be stopped – nor could the new challenges it raised. The same is true of today's digital innovations.
In terms of security, we need to ensure we aren't sitting, as it were, on a slow-moving canal barge while the 8:15 to Manchester flashes past. We need to take a clear-eyed look at the strategic vulnerabilities that these technologies will bring, and anticipate how malicious actors could attempt to exploit new digital infrastructure for their own gain or to deploy it as a weapon.
Some challenges, such as the misuse of social media to spread disinformation – using, among others, cutting-edge tools like deepfake videos and artificial intelligence – are already clear. But, crucially, we are also talking in terms of the infrastructure itself.
The fifth generation of mobile communication technology, 5G, poses a particular challenge. It will be the backbone of global connectivity, which raises strategic and security questions about issues like supply-chain security and provenance. How can we be sure that the components used in future generations of European technology – not only 5G – will be secure?
Already, digital supply-chain security is far from airtight, reflected in recent reports of companies finding mysterious chips on their server motherboards, seemingly added at the time of manufacture. The British government has warned telecoms companies to consider their suppliers very carefully, while the United States has been looking to restrict some kinds of foreign direct investment in key technologies like semiconductors and robotics.
To secure the digital-infrastructure supply chain, we need greater transparency regarding the provenance of technological components. Maintaining a diversity of suppliers is also vital.
Furthermore, common standards and rules are needed to establish the trustworthiness of international partners. That is the basis of a recent proposal by French President Emmanuel Macron, described in the Paris Call for Trust and Security in Cyberspace.
The Internet, according to Macron, has become a site of conflict, where malicious actors exploit the vulnerabilities of digital products and services. He proposes that Europe create an "Internet of Trust," based on lawfulness and cooperation. I agree. Europeans should be able to continue enjoying their online lives secure in the knowledge that their fundamental values and rights, such as free speech, are protected.
We need a strategy that balances our need to harness technological innovation in order to safeguard our economic future with the need to avoid creating massive security vulnerabilities in the process. And with the technology train already hurtling down the track, we must act fast to mitigate current risks and ensure that we are laying the groundwork needed to avoid future threats.
In confronting this admittedly huge challenge, we need to avoid short-sighted responses, such as protectionism and other measures that stifle innovation. Instead, we must map the scale and extent of the risk, and decide what is truly strategic. For Europe, this means not just protecting supply chains, but also pursuing large-scale, coordinated investment in our own tech industries. The European Commission is uniquely well placed to drive this cross-sectoral work.
It is not too late for Europe to safeguard its digital future. Even the owner of the Bridgewater Canal eventually saw the way the wind was blowing – and invested heavily in the rival railway.
Julian King, a former British ambassador to Ireland and France, is the EU Commissioner for the Security Union.

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pity the person who invests heavily in canals right before the railways start operating. you could understand, for example, why the sponsor of the bridgewater canal (perhaps england's first) would vehemently oppose the planned liverpool and manchester railway. but the march of technology could not be stopped – nor could the new challenges it raised. the same is true of today's digital innovations.
in terms of security, we need to ensure we aren't sitting, as it were, on a slow-moving canal barge while the 8:15 to manchester flashes past. we need to take a clear-eyed look at the strategic vulnerabilities that these technologies will bring, and anticipate how malicious actors could attempt to exploit new digital infrastructure for their own gain or to deploy it as a weapon.
some challenges, such as the misuse of social media to spread disinformation – using, among others, cutting-edge tools like deepfake videos and artificial intelligence – are already clear. but, crucially, we are also talking in terms of the infrastructure itself.
the fifth generation of mobile communication technology, 5g, poses a particular challenge. it will be the backbone of global connectivity, which raises strategic and security questions about issues like supply-chain security and provenance. how can we be sure that the components used in future generations of european technology – not only 5g – will be secure?
already, digital supply-chain security is far from airtight, reflected in recent reports of companies finding mysterious chips on their server motherboards, seemingly added at the time of manufacture. the british government has warned telecoms companies to consider their suppliers very carefully, while the united states has been looking to restrict some kinds of foreign direct investment in key technologies like semiconductors and robotics.
to secure the digital-infrastructure supply chain, we need greater transparency regarding the provenance of technological components. maintaining a diversity of suppliers is also vital.
furthermore, common standards and rules are needed to establish the trustworthiness of international partners. that is the basis of a recent proposal by french president emmanuel macron, described in the paris call for trust and security in cyberspace.
the internet, according to macron, has become a site of conflict, where malicious actors exploit the vulnerabilities of digital products and services. he proposes that europe create an "internet of trust," based on lawfulness and cooperation. i agree. europeans should be able to continue enjoying their online lives secure in the knowledge that their fundamental values and rights, such as free speech, are protected.
we need a strategy that balances our need to harness technological innovation in order to safeguard our economic future with the need to avoid creating massive security vulnerabilities in the process. and with the technology train already hurtling down the track, we must act fast to mitigate current risks and ensure that we are laying the groundwork needed to avoid future threats.
in confronting this admittedly huge challenge, we need to avoid short-sighted responses, such as protectionism and other measures that stifle innovation. instead, we must map the scale and extent of the risk, and decide what is truly strategic. for europe, this means not just protecting supply chains, but also pursuing large-scale, coordinated investment in our own tech industries. the european commission is uniquely well placed to drive this cross-sectoral work.
it is not too late for europe to safeguard its digital future. even the owner of the bridgewater canal eventually saw the way the wind was blowing – and invested heavily in the rival railway.
julian king, a former british ambassador to ireland and france, is the eu commissioner for the security union.


text:
SAN PASCUAL, Masbate -- A village councilor was shot dead after he was fired upon by an unidentified suspect at around 7 p.m. Tuesday.
According to San Pascual Police Station, the victim was identified as Donald Carba, an incumbent barangay kagawad of Mabini, this town.
Punong Barangay Felipe Dela Pena reported to the police that the victim was shot with bullets from a still-unidentified gun caliber.
The suspect fled following the shooting.

lowercased:
san pascual, masbate -- a village councilor was shot dead after he was fired upon by an unidentified suspect at around 7 p.m. tuesday.
according to san pascual police station, the victim was identified as donald carba, an incumbent barangay kagawad of mabini, this town.
punong barangay felipe dela pena reported to the police that the victim was shot with bullets from a still-unidentified gun caliber.
the suspect fled following the shooting.


text:
Sunday – Events at the Skratch – Beginning at 2 PM.
Electricity is on the air!!!
Another enjoyable early afternoon at Cactus with DJ Francois and Master Instructor Tony Rausseo.
Le Skratch has opened its doors to the public on Sundays. This is to the delight of many salsa dancers.
This is, in fact, good news to all the Salseras and Salseros out there. They all can have their “Salsa Fix” early. Then go home and do other things.
The DJ has prepared a special repertoire composed of Salsa, Bachata, Cha-cha, Kizomba and much more. In addition, you will see the latest video clips on the plasma screen. The DJ is planning to light up the place for all of you.
Tony Rausseo and his Hot Latin Salsa team have a bag full of Dance Tricks, fancy and flashy moves. He is ready to share them all with you. You will not leave the dance floor empty handed. There will be a move for everyone. This is, whether not, you are a beginner, an intermediate or an advanced dance student.
Needless to say, that is a perfect opportunity to practice your dance figures, steps and turn patterns.
Finally, you can enjoy a cool drink, outside, on the terrace or at the bar; before going dancing or after or in between. This will cool you off or put you in the mood to keep on dancing Hot Latin Salsa.
Get a crash course in salsa dancing WANT to learn the salsa?

lowercased:
sunday – events at the skratch – beginning at 2 pm.
electricity is on the air!!!
another enjoyable early afternoon at cactus with dj francois and master instructor tony rausseo.
le skratch has opened its doors to the public on sundays. this is to the delight of many salsa dancers.
this is, in fact, good news to all the salseras and salseros out there. they all can have their “salsa fix” early. then go home and do other things.
the dj has prepared a special repertoire composed of salsa, bachata, cha-cha, kizomba and much more. in addition, you will see the latest video clips on the plasma screen. the dj is planning to light up the place for all of you.
tony rausseo and his hot latin salsa team have a bag full of dance tricks, fancy and flashy moves. he is ready to share them all with you. you will not leave the dance floor empty handed. there will be a move for everyone. this is, whether not, you are a beginner, an intermediate or an advanced dance student.
needless to say, that is a perfect opportunity to practice your dance figures, steps and turn patterns.
finally, you can enjoy a cool drink, outside, on the terrace or at the bar; before going dancing or after or in between. this will cool you off or put you in the mood to keep on dancing hot latin salsa.
get a crash course in salsa dancing want to learn the salsa?


text:
QUINCY – The Barnstable High School boys basketball team turned up its defensive intensity in the second half, got better shot selection and key points from the foul line to hand host Quincy a loss in the first round of the Quincy Tournament on Monday.
QUINCY – The Barnstable High School boys basketball team turned up its defensive intensity in the second half, got better shot selection and key points from the foul line to hand host Quincy a 52-56 loss in the first round of the Quincy Tournament on Monday.
The Red Raiders improve to 10-8 and will face Milton today in the second day of the tournament. Quincy will face Southeastern.
The Presidents took advantage of poor shooting in the second quarter by Barnstable to take a 26-21 lead to the half. The Red Raiders turned the tide and led by nine heading to the final quarter, and controlled the fourth en route to the win.
Barnstable notched 19 points from the charity stripe to help hold off Quincy down the stretch.
Charlie Rice led the Red Raiders with 15 points and 14 rebounds. Matt Delaney added 13 points and Justin Blaze added eight points.
Tykei Hallman led the Presidents with 11 points.
WHITMAN – John Stanton had 12 points and two 3-pointers for the Blue Knights.
Whitman-Hanson had six 3-pointers in the first half to build an insurmountable lead.
Sandwich (3-16) faces Plymouth North tonight.
NEW BEDFORD – A strong opening quarter was wasted through costly turnovers as the Barnstable girls basketball team fell to New Bedford in its regular season finale.
The Red Raiders (9-9) will now await their MIAA Div. 1 tournament seeding.
Rogetta Donalson led Barnstable with 12 points, including two 3-pointers while Tayler Wornum (six points, nine rebounds), Cayana Soares (six points, three steals) and Molly Bent (two points, eight assists) propelled the Red Raiders' offense, which closed to within six in the third.
Brianna Perry (14) and Alyssa Roach (15) led for New Bedford.

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quincy – the barnstable high school boys basketball team turned up its defensive intensity in the second half, got better shot selection and key points from the foul line to hand host quincy a loss in the first round of the quincy tournament on monday.
quincy – the barnstable high school boys basketball team turned up its defensive intensity in the second half, got better shot selection and key points from the foul line to hand host quincy a 52-56 loss in the first round of the quincy tournament on monday.
the red raiders improve to 10-8 and will face milton today in the second day of the tournament. quincy will face southeastern.
the presidents took advantage of poor shooting in the second quarter by barnstable to take a 26-21 lead to the half. the red raiders turned the tide and led by nine heading to the final quarter, and controlled the fourth en route to the win.
barnstable notched 19 points from the charity stripe to help hold off quincy down the stretch.
charlie rice led the red raiders with 15 points and 14 rebounds. matt delaney added 13 points and justin blaze added eight points.
tykei hallman led the presidents with 11 points.
whitman – john stanton had 12 points and two 3-pointers for the blue knights.
whitman-hanson had six 3-pointers in the first half to build an insurmountable lead.
sandwich (3-16) faces plymouth north tonight.
new bedford – a strong opening quarter was wasted through costly turnovers as the barnstable girls basketball team fell to new bedford in its regular season finale.
the red raiders (9-9) will now await their miaa div. 1 tournament seeding.
rogetta donalson led barnstable with 12 points, including two 3-pointers while tayler wornum (six points, nine rebounds), cayana soares (six points, three steals) and molly bent (two points, eight assists) propelled the red raiders' offense, which closed to within six in the third.
brianna perry (14) and alyssa roach (15) led for new bedford.


text:
The well-known U.S. company Colore Pantone Institute, which is a provider of professional color standards for design, each year selects the color of the upcoming year.
The rich tangerine color in the interior – it’s an easy way to add a warmth and energy in the house with the help of small accessories, such as pillows, vases, lamps, blankets, coffee tables and many other details in the interior.
Probably, the kitchen is room where we spend the most time. Therefore, it is important that it meets our expectations – functional furniture, beautiful colors, etc. In this article, we’ll give you design ideas for kitchen walls color. If you want to create a pleasant and welcoming atmosphere in your kitchen, you can choose the yellow.
Paint the bedroom walls with the yellow, honey, carrot and apricot tones. This cheerful bedroom will remind you about the rest, charging cheerful mood. Furthermore, by choosing a combination of yellow and orange can be successfully highlight features of various styles. Saturated perky colors (orange, carrot, bright yellow) used in Mexican style.
What do you associate the Scandinavian countries? This is a prolonged and snowy winter, long nights and capricious short summer. Perhaps that is why the Scandinavians, who have lacked the natural colors of nature, love to use patterns with bright saturated colors.

lowercased:
the well-known u.s. company colore pantone institute, which is a provider of professional color standards for design, each year selects the color of the upcoming year.
the rich tangerine color in the interior – it’s an easy way to add a warmth and energy in the house with the help of small accessories, such as pillows, vases, lamps, blankets, coffee tables and many other details in the interior.
probably, the kitchen is room where we spend the most time. therefore, it is important that it meets our expectations – functional furniture, beautiful colors, etc. in this article, we’ll give you design ideas for kitchen walls color. if you want to create a pleasant and welcoming atmosphere in your kitchen, you can choose the yellow.
paint the bedroom walls with the yellow, honey, carrot and apricot tones. this cheerful bedroom will remind you about the rest, charging cheerful mood. furthermore, by choosing a combination of yellow and orange can be successfully highlight features of various styles. saturated perky colors (orange, carrot, bright yellow) used in mexican style.
what do you associate the scandinavian countries? this is a prolonged and snowy winter, long nights and capricious short summer. perhaps that is why the scandinavians, who have lacked the natural colors of nature, love to use patterns with bright saturated colors.


text:
A sensitive reconstruction of an old house can ensure the comfort of modern living without the house losing any of its individuality. The renovations may range from partial construction adaptations up to a complex reconstruction of the family house.
Renovation of a house cannot be performed without official permits. Therefore, a part of our services is also the preparation of the documentation for the zoning and building proceedings and provision of all necessary consents.

lowercased:
a sensitive reconstruction of an old house can ensure the comfort of modern living without the house losing any of its individuality. the renovations may range from partial construction adaptations up to a complex reconstruction of the family house.
renovation of a house cannot be performed without official permits. therefore, a part of our services is also the preparation of the documentation for the zoning and building proceedings and provision of all necessary consents.


text:
The holiday season is filled with joy and tradition, but we Disney fans really know how to do it up, am I right? We have some great ideas that you can use to get your halls decked in a way that would make Uncle Walt proud!
As I’m sure you know, Disney has some amazing ornaments that you can purchase at their parks as well as online. No matter what your style, you can find something amazing that fits your holiday decor.
Shop the Disney Store’s ornament selection here.
Everyone needs a fancy sock to hang for Santa to fill with goodies, right? Might as well make it a Disney one! Disney Store online has some really cute ones that you can even personalize.
Maybe you need a new tree skirt? Try the Mickey and Minnie Victorian tree skirt. It’s a gorgeous rich red that feels very much in the Christmas spirit.
If your family is anything like mine, the holidays just aren’t the same without lots of good things to eat. The Disney Family website has lots of Disney inspired recipes that will help you share your love of Disney and the holidays in the most delicious way possible. Whether you’re in the mood for sweet or savory, they have something that will make your tummy happy. They also have lots of recipes that are allergy friendly.
Now, we realize that this is not the norm as far as holiday gift giving is concerned, but if you really want to blow your kids’ minds, give them a Disney vacation under the tree this year. Hear us out. If you know you’re going to be visiting a Disney park within the next 6 months why not just put it under the tree? All it takes a refundable $200 deposit and you can have a lifetime of family memories all in one place. You can even video your kids’ reaction as they open a box that says “You’re going to Disney!” inside. That’s half the fun! You can check Pinterest for some really creative ways to give them the news.
The Dole Whipped group will be sharing some of their favorite Disney holiday traditions with you on the Facebook page as well as Pinterest so keep your eyes out for that. Happy Holidays, everyone!
The Welcome Show is probably one of the best lesser known shows at the Magic Kingdom. It is a short show featuring the Mayor of Main Street USA (or sometimes other Main Street characters), dancers, and some Disney characters including Mickey himself and ends with some fireworks.
The Mayor of Main Street USA is a lovely character who greets visitors to the Magic Kingdom and gets people excited about the opening of the park. He is joined by some dancers in turn of the century costumes as they dance and sing in front of the Main Street USA Train Station.
When they are done the spectators are treated to the sight of the steam train pulling into the station with a whole cast of characters like Peter Pan and Wendy, Alice and the White Rabbit, Mary Poppins, Chip and Dale, Pluto and Minnie, and of course Mickey. You can also see the Family of the Day. That is right, if you get there early enough and the stars align you could have the chance to be the ones waving back at the crowd.
The music that accompanies the show is catchy (and possibly familiar if you have seen some musicals) and you will find yourself singing or humming along. And it is so much fun to count down to the opening of the Magic Kingdom.
It won’t take a large part of your day but it is the best way to start off right and you may even leave with a tear in your eye.

lowercased:
the holiday season is filled with joy and tradition, but we disney fans really know how to do it up, am i right? we have some great ideas that you can use to get your halls decked in a way that would make uncle walt proud!
as i’m sure you know, disney has some amazing ornaments that you can purchase at their parks as well as online. no matter what your style, you can find something amazing that fits your holiday decor.
shop the disney store’s ornament selection here.
everyone needs a fancy sock to hang for santa to fill with goodies, right? might as well make it a disney one! disney store online has some really cute ones that you can even personalize.
maybe you need a new tree skirt? try the mickey and minnie victorian tree skirt. it’s a gorgeous rich red that feels very much in the christmas spirit.
if your family is anything like mine, the holidays just aren’t the same without lots of good things to eat. the disney family website has lots of disney inspired recipes that will help you share your love of disney and the holidays in the most delicious way possible. whether you’re in the mood for sweet or savory, they have something that will make your tummy happy. they also have lots of recipes that are allergy friendly.
now, we realize that this is not the norm as far as holiday gift giving is concerned, but if you really want to blow your kids’ minds, give them a disney vacation under the tree this year. hear us out. if you know you’re going to be visiting a disney park within the next 6 months why not just put it under the tree? all it takes a refundable $200 deposit and you can have a lifetime of family memories all in one place. you can even video your kids’ reaction as they open a box that says “you’re going to disney!” inside. that’s half the fun! you can check pinterest for some really creative ways to give them the news.
the dole whipped group will be sharing some of their favorite disney holiday traditions with you on the facebook page as well as pinterest so keep your eyes out for that. happy holidays, everyone!
the welcome show is probably one of the best lesser known shows at the magic kingdom. it is a short show featuring the mayor of main street usa (or sometimes other main street characters), dancers, and some disney characters including mickey himself and ends with some fireworks.
the mayor of main street usa is a lovely character who greets visitors to the magic kingdom and gets people excited about the opening of the park. he is joined by some dancers in turn of the century costumes as they dance and sing in front of the main street usa train station.
when they are done the spectators are treated to the sight of the steam train pulling into the station with a whole cast of characters like peter pan and wendy, alice and the white rabbit, mary poppins, chip and dale, pluto and minnie, and of course mickey. you can also see the family of the day. that is right, if you get there early enough and the stars align you could have the chance to be the ones waving back at the crowd.
the music that accompanies the show is catchy (and possibly familiar if you have seen some musicals) and you will find yourself singing or humming along. and it is so much fun to count down to the opening of the magic kingdom.
it won’t take a large part of your day but it is the best way to start off right and you may even leave with a tear in your eye.


text:
Piracy will kill the industry – Jesus did it!
Best way to teach college students.
I can't tell if I like this one, or the otter one.

lowercased:
piracy will kill the industry – jesus did it!
best way to teach college students.
i can't tell if i like this one, or the otter one.


text:
Bioscience Day takes place in the Stamp Student Union at the University of Maryland, College Park. There is a fee to park in the Union Lane Garage.
Take I-95 South to the Capital Beltway (I-495); follow signs to College Park, US Route 1 South (Exit 25). Proceed approximately two miles south on US Route 1 and turn right onto Campus Drive. Continue straight around the circle "M" and travel up the hill. After passing the Stamp Student Union on your right, take a right onto Union Lane. The parking garage will be on your left.
Take US Route 50 to Capital Beltway (I-95/495); go north on I-95/495 to College Park, US Route 1 South (exit 25). Proceed approximately two miles south on US Route 1 and turn right onto Campus Drive. Continue straight around the circle "M" and travel up the hill. After passing the Stamp Student Union on your right, take a right onto Union Lane. The parking garage will be on your left.
Go northeast on Rhode Island Avenue (US Route 1). After entering the state of Maryland, continue on US Route 1 for approximately five miles and travel through the city of College Park. Turn left onto Campus Drive. Continue straight around the circle "M" and travel up the hill. After passing the Stamp Student Union on your right, take a right onto Union Lane. The parking garage will be on your left.
Take the Capital Beltway (I-495) and head north and east. Just after I-495 joins I-95 in Maryland above Washington, D.C., get off at exit 25B towards College Park on US Route 1 South. Proceed approximately two miles south on US Route 1 and turn right onto Campus Drive. Continue straight around the circle "M" and travel up the hill. After passing the Stamp Student Union on your right, take a right onto Union Lane. The parking garage will be on your left.

lowercased:
bioscience day takes place in the stamp student union at the university of maryland, college park. there is a fee to park in the union lane garage.
take i-95 south to the capital beltway (i-495); follow signs to college park, us route 1 south (exit 25). proceed approximately two miles south on us route 1 and turn right onto campus drive. continue straight around the circle "m" and travel up the hill. after passing the stamp student union on your right, take a right onto union lane. the parking garage will be on your left.
take us route 50 to capital beltway (i-95/495); go north on i-95/495 to college park, us route 1 south (exit 25). proceed approximately two miles south on us route 1 and turn right onto campus drive. continue straight around the circle "m" and travel up the hill. after passing the stamp student union on your right, take a right onto union lane. the parking garage will be on your left.
go northeast on rhode island avenue (us route 1). after entering the state of maryland, continue on us route 1 for approximately five miles and travel through the city of college park. turn left onto campus drive. continue straight around the circle "m" and travel up the hill. after passing the stamp student union on your right, take a right onto union lane. the parking garage will be on your left.
take the capital beltway (i-495) and head north and east. just after i-495 joins i-95 in maryland above washington, d.c., get off at exit 25b towards college park on us route 1 south. proceed approximately two miles south on us route 1 and turn right onto campus drive. continue straight around the circle "m" and travel up the hill. after passing the stamp student union on your right, take a right onto union lane. the parking garage will be on your left.


text:
It has been more than 2 years since my last post. Where did I go? What have I been doing?
I lead the design and development of Parabo Press.
We did a lot of house hunting.
I killed at least 5 plants.
I turned 30! And Andy threw me my first ever surprise party.
I sprained my ankle during a snowboarding lesson.
I briefly participated in the gray hair trend. My hair is still recovering.
I grew out my bangs for the first time in 20 years.
Penelope continues to be cute.
I’m back because I want to share my thoughts, ideas, inspiration and photos with other people again. My brain is getting crowded. I upgraded the old blog with a brand new outfit (I know, it’s called a theme) from Solo Pine. It’s so pretty and is a great reflection of how my style has evolved and been elevated. You’ll still find almost all my old posts here, some have aged better than others.

lowercased:
it has been more than 2 years since my last post. where did i go? what have i been doing?
i lead the design and development of parabo press.
we did a lot of house hunting.
i killed at least 5 plants.
i turned 30! and andy threw me my first ever surprise party.
i sprained my ankle during a snowboarding lesson.
i briefly participated in the gray hair trend. my hair is still recovering.
i grew out my bangs for the first time in 20 years.
penelope continues to be cute.
i’m back because i want to share my thoughts, ideas, inspiration and photos with other people again. my brain is getting crowded. i upgraded the old blog with a brand new outfit (i know, it’s called a theme) from solo pine. it’s so pretty and is a great reflection of how my style has evolved and been elevated. you’ll still find almost all my old posts here, some have aged better than others.


text:
I’ve heard some reviews of the Alpine Pullover fitting snug in the chest and arms and I didn’t find that with my size 8. I would have liked to try the size 10 which was not available just to see if it would be a bit roomier in the tummy and midsection area but other then that it was not an awkward fit for me. Mind you, I don’t have built arms or anything but I don’t exactly have skinny arms either. I think the Run Spirit Pullover from last year was only roomier in the lower tummy area and the lower arms area because it had a bit of a slouchyness to the design.
Live Simply Jacket. Quite similar to the Wear With All. I like this better then the WWA because I love the bigger hood and the seam detailing on it. Metal zipper though.

lowercased:
i’ve heard some reviews of the alpine pullover fitting snug in the chest and arms and i didn’t find that with my size 8. i would have liked to try the size 10 which was not available just to see if it would be a bit roomier in the tummy and midsection area but other then that it was not an awkward fit for me. mind you, i don’t have built arms or anything but i don’t exactly have skinny arms either. i think the run spirit pullover from last year was only roomier in the lower tummy area and the lower arms area because it had a bit of a slouchyness to the design.
live simply jacket. quite similar to the wear with all. i like this better then the wwa because i love the bigger hood and the seam detailing on it. metal zipper though.