Duraiz Ahmed Khan,Afia Shahid,Asmaa E. Sherif,Hanan Y. Aati,Muhammad Abdullah,Khalid Mehmood,Musaddique Hussain,Abdul Basit,Bilal Ahmad Ghalloo,Kashif ur Rehman Khan
A detailed biochemical characterization, toxicological assessment and molecular docking studies of Launaea fragilis: An important medicinal xero-halophyte
Launaea fragilis,GC–MS analysis,Toxicity,Antioxidant,Enzyme inhibition,Computational analysis
Graphical abstract
A detailed biochemical characterization, toxicological assessment and molecular docking studies of Launaea fragilis: An important medicinal xero-halophyte



Since the dawn of civilizations, humans and plants both have co-existed and this co-existence has helped humans in revolutionizing pharmaceutical and nutritional sciences as plants have represented the paradigm of remedies for centuries (Sadeer et al., 2019). Recently, the interest of researchers has increased exponentially to acknowledge the composition of plants and investigate their clinical applications. Moreover, utilizing the power of nature to overcome proliferating diseases like diabetes, skin aging, cancer, heart attack and alarming health conditions like COVID-19 (Coronavirus disease 2019) is the need of the hour (Bhalla et al., 2021). Numerous bioactive compounds have been identified and isolated from the plants serving not only as lead drugs but also as precursors in the development of new drug entities (Parveen et al., 2021). Bioactive secondary metabolites are considered a renewable source of drug leads that are non-toxic, environment friendly, and hold extensive uses in pharmaceutical, nutraceutical, perfume, and agricultural industries (Sharma et al., 2021). These metabolites are responsible for the anti-inflammatory, antioxidant, antimicrobial, anti-cancer, anti-diabetic, and numerous other therapeutic properties of the plant. Because of these characteristics about 20,000 different species of plants are used for treatment purposes (Aziz et al., 2022). Due to the immense medicinal potential of plants, botanical drug investigation programs led by the government are initiated in some countries to discover phytopharmaceuticals (Ahn 2017). Nowadays medicinal chemists make use of virtual screening methods to search drug candidates, these methods are rational and direct with the advantage of being cost-effective and efficient. Scientists in pursuit of novel drugs often adopt the molecular docking method that helps in finding bioactive secondary metabolites from the pool. This method studies the theoretic affinity of a specific molecule with a specific biological target (Tripathi and Misra 2017). Virtual screening through molecular docking helps in determining the potential ability of natural products and developing novel herbal products as remedies for various clinical needs (Parveen et al., 2021). Launaea fragilis (Asso) Pau is a medicinal xero-halophyte belonging to the Asteraceae family. The genus launaea Cass. is a polymorphic genus that comprises 54 species and 10 subspecies that are distributed throughout SW Asia, South Mediterranean, and Africa (El-Darier et al., 2021). Many of its plants have folklore uses to treat diarrhea, infected wounds, inflammation, bitter stomachic, skin diseases, child fever, and hepatic pain and are also used for their diuretic, insecticidal and soporific properties (Cheriti et al., 2012). Considering the medicinal importance of the genus launaea Cass. and the ethnobotanical background of L. fragilis, the present study aimed to assess the comprehensive phytochemical make-up, safety or toxicity profile, biological potential, and in-silico computational aspects of ethanolic extract of L. fragilis (EELF) for the first time as there is scarcity of experimental records on this plant. Phytochemical profiling was done with gas chromatography-mass spectrometry (GC–MS) secondary metabolite analysis. To establish safety profile, an in-vivo chronic toxicity study was performed on the chicks. Plants can be applied for multi-targets in treating diseases at a single time. It is possible due to the fact of plant pharmacological and chemical diversity. Therefore, in this study, the biological potential of L. fragilis was assessed through various biological assays including in-vitro antioxidant, antiviral, antibacterial, and various clinically significant enzyme inhibition assays. Moreover, in-silico computational studies of the phytochemicals identified in the EELF were done to highlight the interactions, mechanisms of enzyme inhibition, and ADMET profiles.

The naturally grown whole plant of L. fragilis was procured in November 2020 from Dingarh, Mojgarh, and Derawar fort areas in the Bahawalpur region, Pakistan. The plant was identified as L. fragilis by taxonomist Mr. Ghulam Sarwar, from the Department of Botany, The Islamia University of Bahawalpur, Punjab, Pakistan. The plant sample with the reference number (LS/IUB/163) was also submitted to the official herbarium of the University. The plant was shade dried, powdered (500 g) and the hydroethanolic extract of L. fragilis (EELF) was prepared through maceration in 80% ethanol: water 20% with intermittent shaking for 14 days at room temperature. The macerated plant was filtered and the extract was concentrated at 45 °C and reduced pressure using a rotary evaporator. The extract (53 g) prepared was stored at 2 to 8 °C for further use. The percent (%) yield was calculated on dry weight basis.

EELF was subjected to primary and secondary metabolites screening as described previously by (Velavan 2015) to identify the presence of carbohydrates, lipids, amino acids, proteins, alkaloids, glycosides, saponins, tannins, flavonoids, steroids, terpenes, and resins.

Previously established methods were utilized to determine TFC (total flavonoid content) and TPC (total phenolic content) respectively (Ahmed et al., 2022). The TFC was reported as mg Quercetin Equivalents (mg QE/g dry extract), whereas the TPC was reported as mg Gallic Acid Equivalents (mg GAE/g dry extract).

Chemical profiling of EELF was done using GC–MS analysis. Gas chromatogram (Agilent 6890 series) coupled with mass spectrometer detector (Agilent 5973 series) was used. The column used was ultra-inert capillary non-polar HP-5MS with a 30 m column length, 0.25 µm film thickness, and 25 mm internal diameter. Helium gas (99.995%) in the pure form was employed to carry the sample at a constant 1.02 mL/min flow. 1.0 µL of the hydro-ethanol extract diluted with a respective solvent (ethanol) was injected at 245 °C using split-less injection. Initially, the temperature was adjusted at 55–155 °C and then gradually elevated at 3 °C/min with a retention time of 10 min. In the end, 310 °C temperature at 10 °C/min was adjusted. Detected bioactive compounds were identified through a NIST library search (NIST 2011) (Hayat and Uzair 2019).

Toxicological evaluation of different doses of EELF was conducted on a-day-old broiler chicks (n = 100) procured from Faisal Chicks (Pvt) Ltd, Multan using standard protocols. The study was approved by Pharmacy Animals Ethics Committee (PAEC) with reference number PAEC/21/132. The study was executed in November and December 2021 where these chicks were kept in a semi-open shed under standard conditions of temperature, humidity, feed, and management. Vaccinations of all the chicks were done as per schedule against Infectious Bursal disease (IBD), Hydropericardium (HPS), and Newcastle (ND). A starter diet having 18% crude protein and 2700 Kcal/kg metabolizable energy along with clean water was initiated ad libitum. All the chicks were randomly divided into five groups (G1-G5) after seven days of acclimatization and each group was separated from the other in a 3x3 feet pen covered with an iron mesh. Each group was treated with a different dose of EELF on daily basis for the next 21 days as provided in Table S1.

The toxicity investigations of EELF were conducted through already established methods with slight modifications (Shahzad et al., 2012, Ghaffar et al., 2017, Saleem et al., 2019). All the experimental birds were examined three times a day for any irregularity and a record was maintained. Clinical signs, behavioral changes, stimuli response, physical appearance, and mortality were observed. Feed consumption along with water intake was also observed. On days 7, 14, and 21 after the commencement of dosing, blood samples were drawn from the chicks for serum biochemical and hematological examinations. On the same sampling days, five birds from each group were weighed to obtain live weight and then slaughtered to obtain carcass and different body organ weights.

A hematological examination was performed through an automated hematological analyzer and the parameters considered were leucocytes, lymphocytes, erythrocytes, hemoglobin, HCT, MCHC, and MCV count. For serum biochemical analysis, serum was separated by centrifugation at 3000 rpm for 15 mins and stored at 8℃. AST, ALT, ALP, Urea, and serum creatinine were determined through commercially available kits.

The chicks were slaughtered by cutting the jugular vein. Carcass weight along with visceral organ weights (absolute weight) and organ indexes (Organ weight/ Body weight) were calculated.

Radical scavenging activity quantification (RSA) was done through ABTS (2,2-azinobis 3-ethylbenzothiazoline-6-sulfonic acid) and DPPH (1,1-diphenyl-2-picrylhydrazyl). The reducing capability of EELF was determined using FRAP (ferric-reducing antioxidant power) and CUPRAC (Cupric-reducing antioxidant capacity). Already established methods were used with slight modifications.

Plant extract (2500 μL) and 2500 μL ethanolic extract of DPPH (0.004%) were mixed and then incubated in the dark at 25 °C and stirred for 20 min. For baseline correction, 90% aqueous ethanol was prepared as a negative control, and absorbance was taken at 517 nm. The outcomes were measured as IC50 value (Shahzad et al., 2022).

ABTS+ cation was produced by incubating potassium persulfate (2.5 mM) and ABTS mixture (7 mM) in the absence of light at room temperature. Plant extract (75 µL) was mixed with ABTS+ solution (150 µL) and the absorbance was taken at 734 nm after 30 mins (Grochowski et al., 2019). The results were measured as IC50 value.

Plant extract (75 µL) was mixed with [7.5 mM neocuprine (150 µL), 10 mM CuCl2 (150 µL), 1 M NH4Ac buffer (150 µL)] mixture, at 450 nm absorbance was taken after 30 mins (Grochowski et al., 2019). The results were measured as IC50 value.

Plant extract (75 µL) was added to the reagent (1500 µL) in 10 mM TPTZ (2,4,6-tris(2-pyridyl)-s-triazine) in Hydrochloric acid (40 mM), 20 mM ferric chloride and acetate buffer (0.3 M) in a final 10:1:1 ratio. At 593 nm absorbance was noted after 30 min (Khursheed et al., 2022). The results were measured as IC50 value.

The inhibition potential of EELF was tested against α-glucosidase, tyrosinase, and acetylcholinesterase. Eserine, kojic acid and agarose were used as standards for acetylcholinesterase, tyrosinase, and α-glucosidase respectively.

α-glucosidase inhibitory potential of EELF was determined using an already established method (Palanisamy et al., 2011) with minute changes. The test was performed using a 96-well microplate, with agarose as standard and P-NPG (P-nitrophenyl-D-glucopyranoside) as a substrate. α-glucosidase (0.4 U/mL) was dissolved in a phosphate buffer solution having pH 6.8 and BSA(0.2%) added to it. The sample was prepared by mixing plant extract (40 μL), P-NPG (40 μL), and α-Glucosidase solution (40 μL) in phosphate buffer (pH6.8) in 96 well microplate and incubating at 37 °C for 20 min. At 400 nm, absorbance was observed and the IC50 value was determined.

The spectrophotometric procedure developed by (Ellman et al., 1961) was employed to analyze the acetylcholinesterase inhibitory potential as formerly described by (Aktumsek et al., 2013) with minute changes. The test was performed using a 96-well microplate, Eserine as a positive control and acetylthiocholine iodide as a substrate. Fifty μL sample extract (2 mg/ml), 125 μL 0.3 mM 5,5′-Dithio-bis(2-nitro- benzoic) acid in 50 mM Tris–HCl (pH 8) and 20 μL of acetylthiocholine iodide (1.5 mM) were taken in 96 well microplate. Absorbance at 405 nm was taken after every 45 s. Thereafter, 25 μL acetylcholinesterase was mixed with it. The absorbance was measured at 405 nm after 15 mins and the IC50 value was calculated.

The Dopachrome method established by (Ghalloo et al., 2022) with some changes was used. The test was performed using a 96-well microplate, Kojic acid as standard and L- DOPA as a substrate. The sample was prepared by mixing 25 μL plant extract, phosphate buffer (100 μL), and tyrosinase solution (40 μL) in 96 well microplate. Thereafter, 40 μL L-DOPA was mixed with it. At 492 nm, the absorbance was observed after 15 mins of incubation at 37 °C and IC50 was calculated.

In-vitro Antibacterial potential of EELF was examined against gram-positive strains including Micrococcus luteus (ATCC 4925), Bacillus pumilus (ATCC 13835), Bacillus subtilis (ATCC 1692), Staphylococcus aureus (ATCC 6538), Staphylococcus epidermidis (ATCC 8724) and gram-negative strains including Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 9027). Drug Testing Laboratory Punjab, Pakistan provided these strains. The Standard drug used was a combination of Amoxicillin/Clavulanic acid.

Already established protocols as described by were used (Shahid et al., 2023). Bacterial cultures were prepared by streaking on Mueller Hinton agar plates and incubating at 37℃ for 24 h. After a day, a few colonies of each bacteria were added to sterile nutrient broth in a test tube and incubated at 37℃ overnight. In the end, dilutions of 108 CFU/mL cell density were prepared for each bacterial colony. Sample solutions of EELF were prepared at concentrations of 50,100 and 150 mg/mL by dissolving the extract in 10% DMSO (Dimethyl sulfoxide) solution. The bacterial inoculum (0.1 mL) was spread evenly throughout the surface of Mueller Hinton agar (30 mL) in a petri dish. Four wells having 5 mm diameter were punched into the plates and a sample solution (80 µL) was added to each well. The Petri dishes were incubated for 24 h at 37℃ and the results were computed as Zone of Inhibition expressed in millimeters.

The anti-viral potential of EELF was assessed according to protocols established by (Dilshad et al., 2022) with slight modifications. Three strains include Newcastle disease virus (NDV, Lasoota strain), Avian infectious Bronchitis Virus (IBV, H120 strain), and Influenza Virus (H9, H9N2 strain).

Chicken eggs possess numerous advantages as it is readily available, easy to handle, cheap, and require little storage space hence, are used for preliminary viral growth. Chorioallantoic membrane fluid provides optimum conditions for viral replication. Specific pathogen-free 8–12 days old chicken embryonated eggs were inoculated with above mentioned viral strains. Before inoculation, eggs were sterilized with a spirit swab, and with the help of a 3 cc syringe, a hole was made and viral strains were injected into embryonated eggs. After inoculation, melted wax was used to seal holes and inoculated eggs were incubated for 72 h at 37℃. Allantoic fluids were harvested (after 72 h) with the help of a syringe and subjected to Hemagglutination Test (HA) to compute the titer of each virus using a 96-well microtiter round bottom plate.

Hemagglutination (HA) assay was performed by collecting 5 mL of chicken blood using Alsever’s solution. Blood was centrifuged at 4500 RPMs for 4–5 mins and the supernatant was disposed of. The process was repeated thrice for better purification and results. RBC solution (1%) was prepared by mixing 20 µL packed RBCs with phosphate buffer saline solution (2 mL) and shaken gently in Eppendorf tubes to avoid precipitation. PBS (40 µL) was poured into each microtiter plate well and samples (40 µL) were added to the first well, mixed thoroughly, and 40 µL of this solution was transferred to 2nd well, and the process repeated up to the 11th well while 12th well only contained PBS (negative control). RBC solution (40 µL) was mixed in each well, incubated for an hour at 37℃, and Hemagglutination activity was observed.

Different tools like PyRx, Babel, auto dock vina software, Discovery studio, and MGL Tools were used to carry out molecular docking studies. PubChem database was used to download the structures (3D) of the ligand molecules in SDF and were optimized using Babel. The optimized ligand molecules were docked with three enzymes namely acetylcholinesterase, α-glucosidase, and tyrosinase. The co-crystal structures of α-glucosidase with a code 5ZCB (Auiewiriyanukul et al., 2018), acetylcholinesterase with a code 1GQR (Bar-On et al., 2002), and tyrosinase with a code 3NM8 (Sendovski et al., 2011) were downloaded from protein data bank (PDB). Before docking enzymes were prepared using Discovery Studio 2021 Client and all co-crystallized molecules, water molecules, and inhibitors were removed. Docking was performed by uploading prepared enzymes and ligands to vina, embedded in PyRx. The outcomes were expressed as binding energies and the docked ligands along with their intermolecular interactions with the active sites were visualized using Discovery Studio 2021 Client (Nisar et al., 2022).

On 7st january 2023, SwissADME (https://www.swissadme.ch/), was used to determine ADME attributes of bioactive compounds (Daina et al., 2017) and PROTOX II (https://tox-new.charite.de/protox_II/) was used to determine the toxicity profiles of the bioactive compounds.

Each evaluation was performed thrice to calculate their mean. The outcomes were presented as mean ± SD. SPSS (v22.0) software was operated for analyzing data, one-way analysis of variance and then post hoc Tukey’s test were performed. p < 0.05 was considered as significant.

The powdered material of L. fragilis soaked in hydroethanolic solvent system (Ethanol 80: 20 Distilled water) yielded an extract of 53 g. The percent yielded was calculated on dry weight basis which was 10.6 %. EELF was qualitatively screened for the primary and secondary phytoconstituents. The results of the preliminary phytochemical profiling of EELF are summarized in Table S2.

In the current study, EELF was tested for total bioactive content (TPC and TFC). The results are expressed in Table 1. which showed that EELF had 73.45 ± 0.25 mg QE/g TFC and 109.02 ± 0.23 mg GAE/g TPC.

GC–MS analysis of EELF was done and the results are shown in Table 2. The chromatogram showed 47 peaks (Fig. 1). These compounds were tentatively identified through the NIST library. Major classes of compounds were steroids, fatty acids, esters, and vitamins. β-Amyrin (34.25%) (46), Alpha-Linoleic acid (13.13%) (11), β-Gurjunene (11.77%) (10), Bicyclogermacrene (3.69%) (36), β-Sitosterol (3.43%) (45), Friedelan-3-one (3.40%) (16), 1-ethenyl-1-methyl-4-methylene-2-(2-methyl-1-propenyl)-Cycloheptane (3.01%) (42), Ursa-9-(11),12-dien-3-ol (2.52%) (44), 2,2,6-Trimethylcyclohexanone (1.81%) (29), Ergosta-4,6,22-trien-3-beta-ol (1.68%) (31), 9,17-Octadecadienal, (Z)- (1.57%) (12) and Myristic acid (1.51%) (5) were among the major identified compounds. Overall, the compounds obtained were from different Chemical classes and showed different percent areas as shown in Table 2. In detail the terpenoids showed highest percent area (40.97 %), followed by fatty acids (16.79 %), ketones (16.43%), hydrocarbon (6.48%), sterols (5.11 %), cholesterol derivatives (3.99 %), terpenes (3.69 %), esters (2.94 %), Benzene and its derivatives (1.29 %), carboxylic acids (1.4 %), cyclic diols (0.78%), alkaloids (0.49%), and imidazole (0.34%). The diversity in chemical classes might be the reason of pharmacological potential of the species.

The safety profile of EELF was established through an in-vivo toxicity study on chicks divided into five groups. Different parameters such as hematological, biochemical, and physical responses were assessed during the study with a sampling interval of 7 days. The chicks showed no behavioral alteration or clinical signs on receiving treatment. The outcomes are notified in Table S3. The chicks of each group showed no depression, dullness, tremors, gasping, alteration in feed or water intake, weight loss, or morphological changes. During the experiment period (21 days) of continuous treatment, no deaths or toxicity symptoms were observed. The effects of EELF on the chick’s hematopoietic system and biochemical parameters after oral administration were examined and results are summarized in Table S4 and S5. Haematological (hemoglobin, erythrocytes, MCHC, leucocytes, lymphocytes, and HCT) and biochemical (ALT, AST, Serum urea, ALP, and creatinine) parameters of the control and all the experimental groups showed no significant difference in the values. Effect of EELF after oral administration was also observed on the live weight, carcass weight, and organs indexes. The results are tabulated in Table S6. No significant difference in weights and injuries to any organs were observed even at higher doses.

In this study, EELF was tested for its antioxidant potential through four different assays Table 1. The extract had an IC50 value of 39.27 ± 0.90 and 26.87 ± 1.21 µg/mL for DPPH and ABTS, respectively. Whereas, the IC50 values of 13.14 ± 0.18 and 18.06 ± 0.34 µg/mL for CUPRAC and FRAP, respectively.

The EELF presented significant enzyme inhibition and outcomes are tabulated in Table 3. For α-glucosidase, EELF showed an IC50 value of 36.53 ± 0.05 µg/mL in comparison to agarose (standard) 5.87 ± 0.01 µg/mL. For acetylcholinesterase, EELF showed an IC50 value of 33.28 ± 0.08 µg/mL in comparison to Eserine (standard) 1.87 ± 0.01 µg/mL. EELF showed an IC50 value of 27.85 ± 0.04 µg/mL against tyrosinase, while the standard Kojic acid showed an IC50 value of 2.87 ± 0.01 µg/mL.

The antibacterial potential of L. fragilis was screened against two gram-negative and five gram-positive strains. Results are shown in Table 4. as the zones of inhibition which suggests dose-dependent inhibition of bacterial growth by the EELF. The extract showed a maximum zone of inhibition of 23 mm at 150 g/ml against micrococcus luteus and was active against other strains as well.

A hemagglutination test was employed to test the anti-viral capabilities of L. fragilis against H9, NDV, and IBV strains. The results are expressed as Titer count as shown in Table 4. EELF showed very strong antiviral activity similar to Acyclovir (standard) against all the tested strains as their titer count was 00.

To highlight vital molecules and possible underlying mechanisms/interactions involved in the enzyme’s inhibition, docking studies were performed with the secondary metabolites tentatively identified in the EELF through GC–MS. Docking of control ligands was done to detect the active site of enzymes. All the tentative compounds were docked with each enzyme and compounds with the least binding energies (kcal/mol) are tabulated along with their interaction site. The results obtained after docking are represented in Table S7. In the case of α-glucosidase, agarose (standard) had a binding energy of −6.8 kcal/mol. whereas, β-Amyrin and Pseudotaraxasterol showed −8.4 kcal/mol, and Friedelan-3-one showed −7.9 kcal/mol. Molecular interactions of these compounds with α-glucosidase are represented in Fig. 2. Docking studies of Acetylcholinesterase showed that Eserine (standard) had a binding energy of −8.9 kcal/mol and Ergosta-4,6,22-trien-3.-beta.-ol showed the least binding energy (-12 kcal/mol) followed by beta-Sitosterol (-11.6 kcal/mol) and beta-Sitosterol acetate (-11.4 kcal/mol). Fig. 3. represents the molecular interaction between these compounds and acetylcholinesterase. In the case of tyrosinase, Kojic acid (standard) showed a binding energy of −5.7 kcal/mol. Whereas a-Neooleana-3,12-diene showed even better binding energy (-8.3 kcal/mol). Followed by Pseudotaraxasterol (-8.1 kcal/mol) and β-Amyrin (-8.0 kcal/mol). Molecular interactions of these compounds with tyrosinase are represented in Fig. 4.

The results of ADMET studies are compiled in Table S8 and S9. All the compounds showed only 1 violation of Lipinski’s rule, showing that they are orally bioavailable drugs. Whereas Benzo[b]naphtho[2,3-d]furan violated zero criteria and possessed high gastrointestinal absorption and blood–brain barrier penetration. The bioavailability radar is shown in Fig. 5. The results of in silico toxicity prediction through ProTox-II compiled in Table S10. showed that all the best-docked compounds have low toxic potential. Whereas, β-Amyrin and pseudotaraxasterol are non-toxic.

Primary and secondary metabolites produced by plants govern important functions for plants, for instance, primary metabolites oversee photosynthesis, growth, development, and respiration. Whereas, secondary metabolites are useful in ethnomedicinal perspectives (Crozier et al., 2008). For instance, alkaloids possess anti-bacterial, anti-fungal, antiviral, and anti-tumor activities (Cahlíková et al., 2020). Tannins, phenols, and flavonoids possess antioxidant and antitumor potential (Muniyandi et al., 2019). Saponins have anti-inflammatory, Antidiabetic, antibacterial, and antitumor activity. The preliminary phytochemical profiling of EELF showed that extract is a great source of these primary and secondary metabolites. Owing to the presence of these bioactive metabolites, L. fragilis can be considered an important therapeutic plant. The extract also revealed a high value of TPC and TFC however, a higher quantity of TPC as compared to TFC was present in the extract. Hence, the ethanolic extract is proved to be a suitable solvent in the preparation of plant extracts with rich polyphenolic content. Therefore, the pharmacological evaluation of ethanolic extracts is of prime significance due to high polyphenolic content. GC–MS analysis is widely applied for the tentative identification of secondary metabolites present in plant extracts (Yousuf et al., 2022). In recent times, the use of this efficient tool has increased and has provided a vital scientific platform for the chemical profiling of medicinal plants (Aye et al., 2019). In the present analysis, GC–MS profiling of EELF showed 47 compounds. Many of these identified compounds have been reported to possess medicinal properties for example, β-Amyrin possesses antihyperglycemic, hypolipidemic agents, and anti-tyrosinase activity (Nogueira et al., 2019, Viet et al., 2021). Beta-Sitosterol is usually used in hypercholesterolemia, heart problems, cancer, neuroprotection, and diabetes (Saeidnia et al., 2014). Friedelan-3-one is an antidiabetic agent (Harley et al., 2021). The pharmacological activities of EELF may be due to these identified compounds so we planned to establish a safety profile and analyze the antioxidant, enzyme inhibition (antidiabetic, skin, and neuroprotective), antibacterial and antiviral potential of L. fragilis. Hematological parameters are one the most essential indicators of human and animal physiological and pathological status as these parameters are targeted by chemical toxins (Nghonjuyi et al., 2016). In toxicological studies hematopoietic system plays a vital role in assessing toxicity, while blood analysis is appropriate for the evaluation of risk as the abnormalities in the values of animal blood can be extrapolated to predict toxicity in humans (Olson et al., 2000). The hematological analysis of chicks showed that different doses of EELF have no detrimental effect on the blood system. However, a small increase in lymphocytes and leucocytes count of the groups treated with EELF after 2 weeks of administration was observed, which suggests that EELF contains bioactive components capable of boosting the immune system as the increase in leucocytes indicates the boosting of the immune system (Gill and Rutherfurd 2001). In addition, the biochemical parameters, body weight, and organ indexes of treated chicks were also examined at the same intervals. Oral administration of EELF for continuous 21 days showed no significant difference in the biochemical parameters, Body weight and organ indexes. Hence, EELF can be considered safe and non-toxic because no significant deteriorative changes in the experimental chicks as compared to control were observed throughout the toxicological evaluation. Radicals with unpaired electrons and the capability to damage biomolecules are termed reactive oxygen species (ROS). ROS can also damage DNA resulting in mutations and different diseases (Halliwell, 1990, Halliwell, 1995, Alho and Leinonen, 1999, Martelli and Giacomini, 2018). Antioxidants considerably delay or stop oxidative stress and discovering novel antioxidants from natural sources is of great importance as they block oxidation, hence preventing oxidative stress-related diseases (Zengin et al., 2018, Basit et al., 2022). The extract showed strong antioxidant activity correlating to polyphenolic content present in the extract (i.e. higher the polyphenolic content stronger the antioxidant) (Chavan et al., 2013). The antioxidant potential of EELF might be the underlying mechanism responsible for its pharmacological activities, as the involvement of ROS in the pathophysiology of several diseases including diabetes, ulcer, and aging is well established (Ahmad et al., 2019). To combat global health-associated crises, the inhibition of clinically important enzymes is a vital strategy. α-glucosidase is a clinically important enzyme that is present in GIT and is involved in carbohydrate digestion hence regulating blood glucose levels (Mollica et al., 2019). The unchecked elevated level of blood sugar can result in diabetes, which can cause kidney, cardiovascular, and eye disorders. Therefore, α-glucosidase inhibition is of vital importance for curing diabetes (Bhandurge et al., 2010). The extract showed an IC50 value of 36.53 ± 0.05 µg/mL and agarose (standard) gave IC50 value of 5.87 ± 0.01 µg/mL. An earlier study on the ethanolic extract of L. nudicaulis showed a hypoglycemic effect on diabetic rats at a dose of 250 and 500 mg/kg/day (El-Newary et al., 2021). The presence of compounds such as β-Amyrin, β-Sitosterol, Friedelan-3-one, etc. is interrelated to the anti-diabetic activity of the extract. Likewise, acetylcholinesterase is another important enzyme that converts acetylcholine into choline and acetic acid hence playing a key part in Alzheimer’s disease (AD) pathogenesis (García-Ayllón et al., 2011). AD is a neurodegenerative disease causing irreversible damage to the brain. As a result, cholinergic neurons are affected and neurotransmission is terminated (Ritter 2012). Recent experiments revealed that inhibition of AChE enhances cholinergic neurotransmission in CNS and treat AD (Bursal et al., 2021). Commercially available synthetic drugs currently in use for treating these diseases have limitations associated with their adverse reactions (Zengin et al., 2016, Zengin et al., 2017). In the current research, EELF showed an IC50 value of 33.28 ± 0.08 µg/mL, while the Eserine (standard) gave 1.87 ± 0.01 µg/mL. Previous research work showed that the methanolic extract of L. procumbens possesses cholinesterase inhibition activity at 100 and 200 mg/kg B.W. (Khan 2012). Moreover, triterpenoids and sterols present in the ethyl acetate extract of S. maritima provide its neuroprotective activity against neuroinflammation occurring in neurodegenerative diseases (George et al., 2020). GC–MS of EELF also showed the presence of sterols and triterpenoids emphasizing the neuroprotective property of the plant and potential treatment for neurodegenerative diseases. EELF was also tested against tyrosinase involved in the production of melanin. Tyrosinase plays a vital role in skin disorders and its inhibitors are beneficial in treating hyperpigmentation in humans (Parvez et al., 2006). EELF showed an IC50 value of 27.85 ± 0.04 µg/mL against tyrosinase and Kojic acid (standard) showed an IC50 value of 2.87 ± 0.01 µg/mL. Tyrosinase inhibition activity is correlated to the presence of polyphenolic compounds as it is reported earlier that polyphenolic compounds bind reversibly to tyrosinase enzyme and reduce its catalytic activity (Chang 2009). EELF also contains polyphenols contributing to its tyrosinase inhibition activity. Conclusively, EELF is rich in antioxidants and secondary metabolites making it inhibitor of clinically important enzymes. GC–MS analysis revealed that EELF contains many compounds which may synergistically be responsible for its antibacterial and antiviral potential namely Octadecanoic acid (Jasim et al., 2015), β-caryophyllene (Tariq et al., 2019), alpha-Linoleic acid (Krishnaveni et al., 2014), Phthalic acid (Huang et al., 2021), etc. as they all possess antibacterial and antiviral activities. Hence, the plant extract was subjected to antibacterial and antiviral assays. L. fragilis showed significant antimicrobial activity as the zones of inhibition computed against all the tested strains were greater than 9 mm. As for the antiviral assay, EELF showed very strong activity even comparable to Acyclovir (standard) with a titer count of 00 against all tested strains. Computational chemistry, especially in-silico molecular docking is a reliable and accurate tool for predicting the interaction of ligands with targeted molecules, their binding energy, underlying mechanisms and correlating the biological activities of therapeutic plants observed in the experiments on a molecular basis (Baig et al., 2018). All the tentative compounds along with standards were docked with acetylcholinesterase, tyrosinase, and α-glucosidase. The inhibition of enzymes was mainly attributed to the formation of Van der Waals, hydrogen bond, pi-alkyl, alkyl, and pi-sigma interactions at the active sites of the enzymes. Compounds like β-Amyrin, Ergosta-4,6,22-trien-3-beta-ol, Pseudotaraxasterol, and Beta-Sitosterol showed even better binding as compared to standards. To determine the drug-likeness, physiochemical properties, and pharmacodynamics of the compounds, the SwissADME was used (Al-Qahtani et al., 2023). ADME properties provide insight whether molecules under study can be used as future medicines or not (Türkan et al., 2021). Compounds having lower molecular weight, Lipophilicity, and lower hydrogen bond capacity possess good absorption, high bioavailability, and distribution (Daina et al., 2014, Duffy et al., 2015). If a chemical compound follows all the criteria of Lipinski’s rule it can show a drug-like behavior and considered as potential therapeutic agent. On the other hand, if a chemical compound fails to follow more than one Lipinski’s criteria it is considered an orally unavailable drug. Lipinski’s rule has the following five criteria: (1) Molecular weight (less than 500); (2) Lipophilicity (Log Po/w less than 5); (3) Molecular refractivity (40–130); (4) Hydrogen bond acceptor (≤10); Hydrogen bond donor (less than equal to 5) (Lipinski 2004). All the best-docked compounds showed only one violation showing that all the compounds are orally bioavailable drugs. To predict toxicity, the PROTOX-II program makes use of the chemical structure and compares it with other chemical compounds with known toxicity (Banerjee et al., 2018). The results of in silico toxicity analysis showed that all the best-docked compounds have low toxic potential. Whereas, β-Amyrin and pseudotaraxasterol are non-toxic.

In this study, L. fragilis ethanol extract (EELF) was explored for the first time for its phytochemical composition, in-vivo toxicity, in-vitro biological potential, and in-silico computational studies. The chemical profiling of EELF showed presence of important primary, and secondary metabolites with pharmacologically significant chemical compounds revealed in the GC–MS analysis. Hence, the extract showed antioxidant, enzyme inhibition, antibacterial, and antiviral activities. The results of in-vivo toxicological evaluation showed the ethanol extract safe after oral administration at different doses for 21 days. The enzyme inhibition ability was also tested theoretically through docking studies, and underlying mechanisms were reported which further supported the enzyme inhibition potential of the plant. ADMET studies for best-docked compounds revealed that these compounds followed Lipinski’s rule rendering them as potential therapeutic agents. Overall, it can be demonstrated that L. fragilis is a safe medicinal plant that can be used as an important source of natural antioxidants, inhibitors of clinically significant enzymes, and antimicrobials that can serve as leads in designing novel phytopharmaceuticals. It is suggested that further studies in the future should be done on the isolation and pharmacological profiling of the compounds tentatively identified in this study.

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.