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704.11, "word": " جاء", "probability": 0.99462890625}, {"start": 704.11, "end": 704.47, "word": " في", "probability": 0.978515625}, {"start": 704.47, "end": 704.97, "word": " هذا", "probability": 0.97119140625}, {"start": 704.97, "end": 705.69, "word": " الفصل", "probability": 0.955322265625}, {"start": 705.69, "end": 706.97, "word": " تستعدوا", "probability": 0.903076171875}, {"start": 706.97, "end": 707.97, "word": " لـ", "probability": 0.752685546875}, {"start": 707.97, "end": 708.35, "word": " Quiz", "probability": 0.429931640625}, {"start": 708.35, "end": 709.09, "word": " اللي", "probability": 0.921875}, {"start": 709.09, "end": 709.87, "word": " هيكون", "probability": 0.887939453125}, {"start": 709.87, "end": 710.45, "word": " الإعلان", "probability": 0.958984375}, {"start": 710.45, "end": 710.81, "word": " خلال", "probability": 0.994140625}, {"start": 710.81, "end": 711.47, "word": " الأسبوع", "probability": 0.9933268229166666}, {"start": 711.47, "end": 712.13, "word": " عليه", 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"probability": 0.938720703125}, {"start": 715.21, "end": 715.73, "word": " القدر", "probability": 0.99462890625}, {"start": 715.73, "end": 715.91, "word": " في", "probability": 0.96337890625}, {"start": 715.91, "end": 716.31, "word": " هذه", "probability": 0.990234375}, {"start": 716.31, "end": 717.27, "word": " المحاضرة", "probability": 0.9775390625}, {"start": 717.27, "end": 718.69, "word": " ألقاكم", "probability": 0.9384765625}, {"start": 718.69, "end": 718.97, "word": " إن", "probability": 0.56689453125}, {"start": 718.97, "end": 719.21, "word": " شاء", "probability": 0.979248046875}, {"start": 719.21, "end": 719.71, "word": " الله", "probability": 0.9541015625}, {"start": 719.71, "end": 720.37, "word": " على", "probability": 0.939453125}, {"start": 720.37, "end": 721.11, "word": " خير", "probability": 0.977783203125}, {"start": 721.11, "end": 721.67, "word": " السلام", "probability": 0.8515625}, {"start": 721.67, "end": 722.21, "word": " عليكم", "probability": 0.954345703125}, {"start": 722.21, "end": 722.73, "word": " ورحمة", "probability": 0.9822998046875}, {"start": 722.73, "end": 723.09, "word": " الله", "probability": 0.97509765625}, {"start": 723.09, "end": 724.11, "word": " وبركاته", "probability": 0.9763671875}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 729.8031875, "duration_after_vad": 715.2625000000007}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/VrAinvqTIMc.srt b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/VrAinvqTIMc.srt
new file mode 100644
index 0000000000000000000000000000000000000000..a292620bc35adffd717c375bc6edf071f747c286
--- /dev/null
+++ b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/VrAinvqTIMc.srt
@@ -0,0 +1,523 @@
+1
+00:00:05,650 --> 00:00:08,130
+أعوذ بالله من الشيطان الرجيم بسم الله الرحمن
+
+2
+00:00:08,130 --> 00:00:11,710
+الرحيم الحمد لله الواحد الأحد الفرد الصمد الذي لم
+
+3
+00:00:11,710 --> 00:00:15,210
+يلد ولم يولد ولم يكن له كفوا أحد والصلاة والسلام
+
+4
+00:00:15,210 --> 00:00:20,010
+على خير الأنام محمد وعلى آله وصحبه الكرام وبعد أهلا 
+
+5
+00:00:20,010 --> 00:00:25,870
+بكم جميعا في لقائنا المتجدد دوما واليوم إن شاء
+
+6
+00:00:25,870 --> 00:00:32,150
+الله نستكمل الحديث عن أقسام الحكم الوضعي ومتعلقاته
+
+7
+00:00:32,150 --> 00:00:37,250
+كنا قد وصلنا إلى القسم الثالث وهو المانعية
+
+8
+00:00:37,250 --> 00:00:43,570
+ومتعلقها المانع والمانعية هي اعتبار الشيء مانعا
+
+9
+00:00:43,570 --> 00:00:50,170
+أما المانع هو الوصف الظاهر المنضبط الذي يلزم من 
+
+10
+00:00:50,170 --> 00:00:57,080
+وجوده عدم الحكم ولا يلزم من عدمه وجود الحكم ولا
+
+11
+00:00:57,080 --> 00:01:01,660
+عدمه خلّينا نشوف المثال هو بالمثال يتضح المقال
+
+12
+00:01:01,660 --> 00:01:07,380
+عندنا مثال القتل طبعا القتل مانع من وجود حكم
+
+13
+00:01:07,380 --> 00:01:14,960
+الوراثة القتل مانع من وجود حكم الوراثة فالقتل
+
+14
+00:01:14,960 --> 00:01:22,730
+المانع من الميراث إذا انعدم لا يلزم من عدمه وجود
+
+15
+00:01:22,730 --> 00:01:29,050
+الحكم وهو الوراثة ولا عدمه لأن المورث قد يكون له
+
+16
+00:01:29,050 --> 00:01:34,570
+مال وقد لا يكون له مال وقد يوجد سبب للوراثة وقد لا
+
+17
+00:01:34,570 --> 00:01:41,830
+يوجد سبب للوراثة إذا المانع هو وصف ظاهر منضبط يلزم
+
+18
+00:01:41,830 --> 00:01:48,630
+من وجوده عدم الحكم ولا يلزم من عدمه وجود الحكم ولا 
+
+19
+00:01:48,630 --> 00:01:55,070
+عدمه زي ما حكينا القتل يمنع من الميراث إذا انعدمه
+
+20
+00:01:55,070 --> 00:02:00,090
+لا يلزم من انعدامه يعني إذا لم يوجد قتل لا يلزم من
+
+21
+00:02:00,090 --> 00:02:05,370
+عدم وجود القتل وجود الحكم وهو الوراثة ولا عدم وجود
+
+22
+00:02:05,370 --> 00:02:11,270
+الوراثة ليش؟ لأن المورث قد يكون له مال وقد لا يكون
+
+23
+00:02:11,270 --> 00:02:18,670
+له مال وقد يوجد سبب للوراثة وقد لا يوجد سبب للوراثة
+
+24
+00:02:18,670 --> 00:02:26,810
+أما القسم الرابع وهو الصحة ومتعلقها الصحيح والصحة
+
+25
+00:02:26,810 --> 00:02:34,010
+هي اعتبار الشيء صحيحا إذا استوفى أركانه وشروطه أما
+
+26
+00:02:34,010 --> 00:02:39,830
+الصحيح هو ما صدر من أفعال المكلف مستوفيا أركانه
+
+27
+00:02:39,830 --> 00:02:46,170
+وشروطه على الكيفية المطلوبة وتترتب عليه أثاره
+
+28
+00:02:46,170 --> 00:02:53,920
+الشرعية زي في العبادات الصلاة إذا استوفت شروطها ووقت
+
+29
+00:02:53,920 --> 00:03:00,000
+المكلف بجميع أركانها تكون صحيحة وذلك لأن آثارها
+
+30
+00:03:00,000 --> 00:03:04,960
+تترتب عليها أما بالنسبة للمعاملات خلّينا ناخد مثال
+
+31
+00:03:04,960 --> 00:03:09,520
+من المعاملات زي البيع الذي استوفى أركانه وشروطه
+
+32
+00:03:09,520 --> 00:03:15,250
+يكون صحيحا وذلك لأنه يترتب عليه انتقال الشيء ..
+
+33
+00:03:15,250 --> 00:03:19,670
+الملكية الشيء المبيع للمشتري وانتقال ملكية الثمن
+
+34
+00:03:19,670 --> 00:03:29,350
+للبائع أما بالنسبة للقسم الخامس وهو البطلان
+
+35
+00:03:29,350 --> 00:03:37,250
+ومتعلقه الباطل البطلان أو الفساد ومتعلقه الباطل أو
+
+36
+00:03:37,250 --> 00:03:42,740
+الفاسد البطلان أو الفساد هو أو هي اعتبار الشيء
+
+37
+00:03:42,740 --> 00:03:50,080
+باطلا أو فاسدا أما الباطل أو الفاسد مصدر من أفعال
+
+38
+00:03:50,080 --> 00:03:55,340
+المكلف غير مستوفن لأركانه وشروطه على الكيفية
+
+39
+00:03:55,340 --> 00:04:01,600
+المطلوبة ولم تترتب عليه آثاره الشرعية خلينا ناخد
+
+40
+00:04:01,600 --> 00:04:05,880
+مثال من العبادات الصلاة الواجبة التي فعلها المكلف
+
+41
+00:04:05,880 --> 00:04:12,330
+وترك ركنا من أركانها أو فعلها بجميع أركانها لكنها
+
+42
+00:04:12,330 --> 00:04:17,370
+كانت قبل دخول وقتها بغير عذر فهذه الصلاة بنحكم
+
+43
+00:04:17,370 --> 00:04:22,490
+عليها بالبطلان أما بالنسبة للمعاملات زي بيع
+
+44
+00:04:22,490 --> 00:04:27,930
+المجنون لشيء يملكه هذا البيع طبعا وإن كان أخذ
+
+45
+00:04:27,930 --> 00:04:32,540
+الصورة الصحيحة الصورة الشكلية الصحيحة لكن يحكم عليه
+
+46
+00:04:32,540 --> 00:04:37,700
+بالبطلان لأنه فقد شرطا من شروطه وهو عدم أهلية
+
+47
+00:04:37,700 --> 00:04:44,060
+البائع ننتقل الآن للفرق بين الباطل و الفاسد أكيد
+
+48
+00:04:44,060 --> 00:04:49,320
+بنسمع في بعض الأحكام نقول هذا باطل و هذا فاسد
+
+49
+00:04:49,320 --> 00:04:55,170
+خلينا نشوف أيش الفرق بين الباطل و الفاسد يطلق
+
+50
+00:04:55,170 --> 00:05:00,110
+الباطل على الفساد عند المتكلمين من علماء الأصول
+
+51
+00:05:00,110 --> 00:05:07,410
+سواء كان في جانب العبادات أو كان في جانب المعاملات
+
+52
+00:05:07,410 --> 00:05:14,770
+كل باطل فاسد وكل فاسد باطل فهما كلمتان مترادفتان
+
+53
+00:05:14,770 --> 00:05:19,850
+تدلان على شيء واحد ما هو هذا الشيء؟ هو عدم ترتب
+
+54
+00:05:19,850 --> 00:05:27,410
+الأثر الشريعي على الفعل إذن عند جمهور العلماء يُطلق
+
+55
+00:05:27,410 --> 00:05:32,690
+الباطل على الفاسد سواء كان في جانب العبادات أو
+
+56
+00:05:32,690 --> 00:05:38,110
+كان في جانب المعاملات كل باطل فاسد وكل فاسد باطل
+
+57
+00:05:38,110 --> 00:05:42,770
+كلمتان مترادفتان تدلان على شيء واحد ما هو هذا
+
+58
+00:05:42,770 --> 00:05:49,470
+الشيء هو عدم ترتب الأثر الشرعي على الفعل طبعا
+
+59
+00:05:49,470 --> 00:05:55,900
+بالنسبة للبطلان والفساد في العبادات لا خلافة في هما
+
+60
+00:05:55,900 --> 00:06:01,620
+بين العلماء فهما بمعنى واحد عند الجمهور أو عند
+
+61
+00:06:01,620 --> 00:06:07,260
+الأحناف وهو مخالفة العبادة لأمر الشارع سواء كانت
+
+62
+00:06:07,260 --> 00:06:13,760
+المخالفة ناشئة عن فوات ركن من أركانها زي الصلاة
+
+63
+00:06:13,760 --> 00:06:17,540
+بدون ركوع أو سجود أو فوات شرط من شروطها كالصلاة
+
+64
+00:06:17,540 --> 00:06:23,590
+بدون وضوء إذا البطلان و الفساد في العبادات لا خلافة
+
+65
+00:06:23,590 --> 00:06:30,550
+بين العلماء أنهم بمعنى واحد طيب هذا بالنسبة
+
+66
+00:06:30,550 --> 00:06:35,350
+للعبادات أما بالنسبة للمعاملات فالحنفية يفرقون بين
+
+67
+00:06:35,350 --> 00:06:41,250
+الباطل و الفاسد ويجعلون للفاسد معنى آخر يخالف معنى
+
+68
+00:06:41,250 --> 00:06:45,760
+الباطل هذا بس في إيه؟ في المعاملات البطلان عند
+
+69
+00:06:45,760 --> 00:06:51,360
+الحنفية هو مخالفة التصرف لأمر الشارع في ركن من
+
+70
+00:06:51,360 --> 00:06:55,600
+أركانه أو أمر من الأمور الأساسية التي تقوم عليها
+
+71
+00:06:55,600 --> 00:07:01,160
+هذه الأركان زي العاقد أو المعقود عليه كالبيع
+
+72
+00:07:01,160 --> 00:07:05,400
+الصادر من مجنون لانتفاء الإرادة المعبر عنها
+
+73
+00:07:05,400 --> 00:07:09,760
+بالإيجاب والقبول وكبيع الميتة والزواج بإحدى
+
+74
+00:07:09,760 --> 00:07:13,720
+المحارم فإنهم يطلقون عليه اسم الباطل ولا يرتبون
+
+75
+00:07:13,720 --> 00:07:20,950
+عليه أثرا شرعيا أما الفساد عند الحنفية هو موافقة
+
+76
+00:07:20,950 --> 00:07:27,270
+التصرف لأمر الشارع في ركن من أركانه والأمور
+
+77
+00:07:27,270 --> 00:07:31,990
+الأساسية التي تقوم عليها تلك الأركان ومخالفته في
+
+78
+00:07:31,990 --> 00:07:38,470
+شرط من الشروط المكملة لذلك زي في المعاملات البيع
+
+79
+00:07:38,470 --> 00:07:42,850
+بثمن مجهول أو إلى أجل مجهول هذا التصرف بيعتبره
+
+80
+00:07:42,850 --> 00:07:49,110
+فاسد ويمكن أن يصحح هذا الشرط ويعود هذا العقد صحيحا
+
+81
+00:07:49,110 --> 00:07:54,510
+طيب السؤال الآن اللي بترح نفسه ما وجه التفرقة عند
+
+82
+00:07:54,510 --> 00:07:58,370
+الحنفية بين العبادات والمعاملات؟ ليش يا أحنا
+
+83
+00:07:58,370 --> 00:08:03,870
+فرقتوا بين الباطل والفاسد في العبادات والمعاملات
+
+84
+00:08:03,870 --> 00:08:08,210
+قلت العبادات الفاسد والباطل واحد لكن المعاملات
+
+85
+00:08:08,210 --> 00:08:17,110
+فرقته بين الباطل والفاسد ليش؟ الجواب أنه لما كان
+
+86
+00:08:17,110 --> 00:08:20,530
+المقصود من العبادات التعبد وهو لا يكون إلا
+
+87
+00:08:20,530 --> 00:08:26,370
+بالامتثال والطاعة طبعا تكون المخالفة فيها مفوتة
+
+88
+00:08:26,370 --> 00:08:31,410
+للمقصود فلا يظهر وجه للتفرقة بين الباطل والفاسد
+
+89
+00:08:31,970 --> 00:08:36,070
+هنا لا تبرأ ذمة المكلف بصلاة فاسدة كما لا تبرأ
+
+90
+00:08:36,070 --> 00:08:44,270
+بصلاة باطلة إذا في العبادة المقصود في العبادة طبعا
+
+91
+00:08:44,270 --> 00:08:49,430
+التعبد والتعبد لا يكون إلا بالامتثال والطاعة ولابد
+
+92
+00:08:49,430 --> 00:08:57,140
+أن يأتي بها على الوجه المطلوب لذلك تكون فاسدة أو
+
+93
+00:08:57,140 --> 00:09:02,020
+تكون .. أو تكون باطلة هذا بالنسبة للعبادة ومن أمثل
+
+94
+00:09:02,020 --> 00:09:08,400
+ذلك صيام يوم العيد نزرا أما في المعاملات طبعا
+
+95
+00:09:08,400 --> 00:09:13,080
+المقصود في المعاملات طبعا تحقيق مصالح العبادة
+
+96
+00:09:13,080 --> 00:09:18,840
+الدنياوية هنا انفسح المجال وأمكنه تحقيقها حتى مع
+
+97
+00:09:18,840 --> 00:09:23,880
+وجود الخلل في وصفها فلا تنعدم وتلغى تماما إلا إذا
+
+98
+00:09:23,880 --> 00:09:28,620
+كان الخلل زي ما حكينا راجع إلى الحقيقة وتؤثر فيها
+
+99
+00:09:28,620 --> 00:09:35,860
+من عدم ركنها الذي هو جزء من ماهيتها أما بالنسبة
+
+100
+00:09:35,860 --> 00:09:42,160
+للقسم السادس وهو العزيمة وهو ما شرع طبعا القسم
+
+101
+00:09:42,160 --> 00:09:48,300
+السادس من أحكام من أقسام الحكم الوضعي العظيمة وهي
+
+102
+00:09:48,300 --> 00:09:54,880
+ما شرع من الأحكام الكلية ابتداء لتكون قانونا عاما
+
+103
+00:09:54,880 --> 00:09:59,080
+لكل المكلفين في جميع الأحوال زي الصلاة والزكاة
+
+104
+00:09:59,080 --> 00:10:04,020
+وسائر الشعائر الإسلامية الكلية أما القسم الأخير
+
+105
+00:10:04,020 --> 00:10:08,980
+وهو الرخصة وهو الحكم الثابت على خلاف الدليل لعذر
+
+106
+00:10:08,980 --> 00:10:14,010
+زي ترك صلاة الجماعة بعذر المطر ترك الصيام في السفر
+
+107
+00:10:14,010 --> 00:10:21,750
+أكل ميت للمضطر بالنسبالي العلماء قسموا الرخصة إلى
+
+108
+00:10:21,750 --> 00:10:27,490
+أقسام جعلوها رخصة واجبة ورخصة مندوبة ورخصة مباحة
+
+109
+00:10:27,490 --> 00:10:31,750
+أما الرخصة الواجبة وهي التي تجب على المكلف أو يجب
+
+110
+00:10:31,750 --> 00:10:37,670
+على المكلف الذي أخذ بها عند قيام موجبها زي إيش؟ زي
+
+111
+00:10:37,670 --> 00:10:43,240
+أكل ميت للمضطر في السفر فإنه يجب عليه أكل ما يسد
+
+112
+00:10:43,240 --> 00:10:48,680
+رمقه ويبقى على حياته إذا اضطر إلى ذلك في السفر
+
+113
+00:10:48,680 --> 00:10:54,740
+طبعا الرمق هو .. هي الروح طبعا مصلحته الآن ابقى
+
+114
+00:10:54,740 --> 00:11:00,120
+على حياته والآن هذه المصلحة تكون واجبة فلا يجوز
+
+115
+00:11:00,120 --> 00:11:06,620
+أن يهدرها إذا تمكن من الحفاظ عليها وذلك بتناول
+
+116
+00:11:06,620 --> 00:11:12,840
+جزء من الميتة تدفع عنه طبعا الضرر أما الرخصة طبعا
+
+117
+00:11:12,840 --> 00:11:17,620
+هنا الرخصة بتكون واجبة لأن الحفاظ على حياته واجبة
+
+118
+00:11:17,620 --> 00:11:20,920
+أما الرخصة المندوبة وهي التي يستحب للمكلف الذي
+
+119
+00:11:20,920 --> 00:11:26,120
+أخذ بها وذلك كالقصر في السفر فإن القصر في السفر
+
+120
+00:11:26,120 --> 00:11:31,400
+الطويل مندوب طبعا وهدف موجود كثير في الأحكام التي
+
+121
+00:11:31,400 --> 00:11:38,400
+تتعلق بالعبادة أما الرخصة المباحة هي التي يجوز
+
+122
+00:11:38,400 --> 00:11:43,680
+للمكلف الذي أخذ بها وذلك كالسلم بيع أحد أنواع
+
+123
+00:11:43,680 --> 00:11:49,260
+البيوع طبعا وهو بيع موصوف في الذمة بثمن عاجل فهو
+
+124
+00:11:49,260 --> 00:11:53,340
+رخصة واردة على خلاف الدليل إلا أنه مباح وليس
+
+125
+00:11:53,340 --> 00:11:57,960
+مندوبا فيجوز للمكلف الذي عمل به إلا أنه لا يندب 
+
+126
+00:11:57,960 --> 00:12:02,630
+أيضًا بيع العارية مثال ثاني للرخصة المباحة
+
+127
+00:12:02,630 --> 00:12:08,190
+وهو بيع الرطب على النخل بالتمر طبعًا هذا البيع 
+
+128
+00:12:08,190 --> 00:12:12,750
+وارد على خلاف الأصل الناهية عن ذلك وورده الإجارة
+
+129
+00:12:12,750 --> 00:12:20,800
+مثل هذا الأمر الآن سنتحدث عن المحكوم فيه نترك هذا
+
+130
+00:12:20,800 --> 00:12:27,500
+الحديث للمحاضرة القادمة بإذن الله و السلام عليكم و 
+
+131
+00:12:27,500 --> 00:12:29,680
+رحمته الله و بركته 
diff --git a/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/_4T4bIvzc8U_raw.json b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/_4T4bIvzc8U_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..ea0ed69edf11c5028b6bed20584b8c162ee26179
--- /dev/null
+++ b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/_4T4bIvzc8U_raw.json
@@ -0,0 +1 @@
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"لكن لماذا اعتبر المشرع هذه الأهلية ناقصة؟ عندي طبعا الجواب سببين السبب الأول التابعية لأمه تابع لأمه التابعية في ارتباطه بامه حسا لتحركه بتحركها وقراره بقرارها فهو جزء منها كاليد والرجل وسائر الأعضاء وارتباطه بيها حكما أيضا اذ يباعوا ببيعها ويعتقوا بعتقها", "tokens": [1211, 19452, 32767, 45636, 995, 1975, 34268, 26890, 9673, 46309, 3615, 29538, 16247, 3224, 1211, 10632, 8717, 995, 4587, 9381, 3660, 22807, 18871, 16254, 23032, 3555, 3615, 995, 25724, 14407, 3555, 8608, 3555, 3555, 9957, 21136, 3555, 3555, 16247, 12610, 16712, 16758, 3615, 10632, 5296, 10721, 2304, 3224, 6055, 16758, 3615, 5296, 10721, 2304, 3224, 16712, 16758, 3615, 10632, 8978, 1975, 43500, 3555, 41193, 3224, 4724, 10943, 3224, 11331, 3794, 995, 5296, 2655, 5016, 31747, 3224, 39894, 5016, 31747, 11296, 4032, 4587, 2288, 9640, 3224, 4724, 4587, 2288, 9640, 11296, 6156, 3224, 2407, 10874, 11622, 38207, 9154, 11296, 9122, 6027, 25708, 16070, 47341, 1211, 46952, 16373, 2288, 16247, 3615, 11242, 16606, 4032, 9640, 2655, 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"word": " حكينا", "probability": 0.9825439453125}, {"start": 895.04, "end": 896.1, "word": " في", "probability": 0.7197265625}, {"start": 896.1, "end": 896.64, "word": " تفصيل", "probability": 0.9493408203125}, {"start": 896.64, "end": 897.12, "word": " لسه", "probability": 0.9288736979166666}, {"start": 897.12, "end": 897.62, "word": " على", "probability": 0.91259765625}, {"start": 897.62, "end": 898.02, "word": " هذا", "probability": 0.85205078125}, {"start": 898.02, "end": 898.76, "word": " الكلام", "probability": 0.97021484375}, {"start": 898.76, "end": 899.0, "word": " عند", "probability": 0.9814453125}, {"start": 899.0, "end": 899.5, "word": " الحديث", "probability": 0.986328125}, {"start": 899.5, "end": 899.84, "word": " عن", "probability": 0.9892578125}, {"start": 899.84, "end": 900.86, "word": " عوارض", "probability": 0.9786376953125}, {"start": 900.86, "end": 901.58, "word": " الأهلية", "probability": 0.9276123046875}, {"start": 901.58, "end": 902.42, "word": " نقف", "probability": 0.9638671875}, {"start": 902.42, "end": 902.84, "word": " الآن", "probability": 0.818603515625}, {"start": 902.84, "end": 903.3, "word": " عند", "probability": 0.98095703125}, {"start": 903.3, "end": 904.08, "word": " أهلية", "probability": 0.9464111328125}, {"start": 904.08, "end": 904.64, "word": " الأداء", "probability": 0.9803059895833334}, {"start": 904.64, "end": 905.22, "word": " لتكون", "probability": 0.96728515625}, {"start": 905.22, "end": 905.52, "word": " في", "probability": 0.974609375}, {"start": 905.52, "end": 906.34, "word": " المحاضرة", "probability": 0.9364013671875}, {"start": 906.34, "end": 907.46, "word": " القادمة", "probability": 0.9943033854166666}, {"start": 907.46, "end": 908.02, "word": " بإذن", "probability": 0.952392578125}, {"start": 908.02, "end": 908.32, "word": " الله", "probability": 0.9609375}, {"start": 908.32, "end": 909.76, "word": " و", "probability": 0.411376953125}, {"start": 909.76, "end": 910.84, "word": " السلام", "probability": 0.927490234375}, {"start": 910.84, "end": 911.42, "word": " عليكم", "probability": 0.955810546875}, {"start": 911.42, "end": 911.6, "word": " و", "probability": 0.99755859375}, {"start": 911.6, "end": 911.94, "word": " رحمة", "probability": 0.8575846354166666}, {"start": 911.94, "end": 912.3, "word": " الله", "probability": 0.97509765625}, {"start": 912.3, "end": 913.3, "word": " وبركاته", "probability": 0.92666015625}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 920.04425, "duration_after_vad": 899.9237500000013}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/_4T4bIvzc8U_raw.srt b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/_4T4bIvzc8U_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..396e1e70812adae5fcf81116376eec5d20c71381
--- /dev/null
+++ b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/_4T4bIvzc8U_raw.srt
@@ -0,0 +1,636 @@
+1
+00:00:04,820 --> 00:00:07,380
+أعوذ بالله من الشيطان الرجيم بسم الله الرحمن
+
+2
+00:00:07,380 --> 00:00:11,200
+الرحيم الحمد لله الواحد الأحد الفرد الصمد الذي لم
+
+3
+00:00:11,200 --> 00:00:15,280
+يلد ولم يولد ولم يكن له كفوان أحد وصلاة والسلام
+
+4
+00:00:15,280 --> 00:00:20,200
+على خير الأنام محمد وعلى آله وصحبه الكرام وبعد
+
+5
+00:00:20,200 --> 00:00:24,780
+بداية أخوات الحبيبات وبنات الطيبات أحييكم بتحية
+
+6
+00:00:24,780 --> 00:00:29,180
+طيبة مباركة من عند الله السلام عليكم ورحمة الله
+
+7
+00:00:29,180 --> 00:00:35,250
+وبركاتهاليوم ان شاء الله هنبدأ بمباحث الفصل الثالث
+
+8
+00:00:35,250 --> 00:00:43,230
+والذي نتحدث فيه عن حقيقة الأهلية وأقسام الأهلية
+
+9
+00:00:43,230 --> 00:00:49,030
+وعوارض الأهلية سواء كان منها العوارض السماوية أو
+
+10
+00:00:49,030 --> 00:00:54,720
+المكتسبةنتحدث أولا عن حقيقة الأهلية طبعا معنى
+
+11
+00:00:54,720 --> 00:01:00,440
+الأهلية في اللغة تطلق على عدة معاني وكلها بترجع
+
+12
+00:01:00,440 --> 00:01:07,620
+لمعنى واحد وهو صلاحية الأمر لشيء يقال أ فلان أهلن
+
+13
+00:01:07,620 --> 00:01:16,340
+لعملي كذا إذا كان صالحا للقيام بيهبالنسبة لمعنى
+
+14
+00:01:16,340 --> 00:01:22,300
+الأهلية في الإصطلاح هي صلاحية الإنسان للإلزام
+
+15
+00:01:22,300 --> 00:01:26,940
+والالتزام، أي هذا الإنسان يكون صالحا لأن تثبت عليه
+
+16
+00:01:26,940 --> 00:01:33,000
+الحقوق وتجب عليه الواجباتهذا الإنسان بيكون صالح
+
+17
+00:01:33,000 --> 00:01:38,780
+لوجوب الحقوق المشروعة له وعليه ولصدور الأفعال منه
+
+18
+00:01:38,780 --> 00:01:44,680
+على وجه يعتد به شرعا طبعا بعد توفر الشروط اللازمة
+
+19
+00:01:44,680 --> 00:01:49,560
+في المكلف لصحة ثبوت الحقوق له والواجبات عليهم
+
+20
+00:01:49,870 --> 00:01:54,590
+هنستعرض عدة تعريفات وبعدين ننتهي إلى التعريف
+
+21
+00:01:54,590 --> 00:01:59,390
+المطلوب بحفظه إلى الامتحان هذا اللي انتوا طبعا
+
+22
+00:01:59,390 --> 00:02:03,930
+بتنتظروه مني خليني نستعرض هذه التعريفات وبعدين نقف
+
+23
+00:02:03,930 --> 00:02:09,590
+على التعريف المطلوب معناه بقى ان بعض التعريفات
+
+24
+00:02:09,590 --> 00:02:14,290
+اللي عرفوها العلماء الأهلية منها صلاحية الإنسان
+
+25
+00:02:14,290 --> 00:02:20,900
+لصدور شيء منه وطلبه منه وقابله إياوأيضا صلاحية
+
+26
+00:02:20,900 --> 00:02:26,420
+الإنسان لثبوت الحقوق له ولغيره وصلاحيته للالتزام
+
+27
+00:02:26,420 --> 00:02:31,380
+بيها وعندنا كمان تعريف الأهلية عبارة عن صلاحية
+
+28
+00:02:31,380 --> 00:02:37,060
+الإنسان لوجوب الحقوق المشروعة له وعليهوفسّروا هذه
+
+29
+00:02:37,060 --> 00:02:43,260
+الصلاحية بالأمانة التي أخبر الله تعالى عنها بقوله
+
+30
+00:02:43,260 --> 00:02:47,820
+إن عرض الأمانة على السماوات والأرض والجبال إلى آخر
+
+31
+00:02:47,820 --> 00:02:54,220
+الأية الأمانة هي هنا فسّرها العلماء بأنها التكاليف
+
+32
+00:02:54,220 --> 00:03:01,380
+الشرعية نستطيع
+
+33
+00:03:01,380 --> 00:03:06,460
+أن نقول أن الأهليةيعني بعد استعراض التعريفات
+
+34
+00:03:06,460 --> 00:03:11,280
+السابقة نستطيع أن نقول هذا هو التعريف المطلوب هذا
+
+35
+00:03:11,280 --> 00:03:18,140
+جمعناه من كل التعريفات وهي عبارة عن صفة قدرها
+
+36
+00:03:18,140 --> 00:03:24,380
+الشارع في الإنسان جعلته موضعا صالحا للمخاطبة
+
+37
+00:03:24,380 --> 00:03:29,280
+بالأحكام الشرعية على جهة الإلزام والالتزام يلا مين
+
+38
+00:03:29,280 --> 00:03:34,810
+حفظت التعريف؟تمام؟ لازم نحفظ التعريف في هذا كل
+
+39
+00:03:34,810 --> 00:03:41,130
+كلمة موجودة في التعريف لازم احنا نحفظها تمام؟ اذا
+
+40
+00:03:41,130 --> 00:03:45,810
+كمان مرة الأهلية وهذا هو كمان مرة بقول التعريف
+
+41
+00:03:45,810 --> 00:03:52,010
+المطلوب هي عبارة عن صفة قدرها الشارع في الإنسان
+
+42
+00:03:52,010 --> 00:03:57,010
+جعلته موضعا صالحا للمخاطبة بالأحكام الشرعية على
+
+43
+00:03:57,010 --> 00:04:01,640
+جهة الإلزام والالتزامأي صلاحية هذا الإنسان لأن
+
+44
+00:04:01,640 --> 00:04:06,340
+تثبت عليه الحقوق وتجيب عليه إيه وتجيب عليه الواجب
+
+45
+00:04:06,340 --> 00:04:12,880
+ننتقل الآن إلى أقسام الأهلية عندي الأهلية تنقسم
+
+46
+00:04:12,880 --> 00:04:18,580
+إلى قسمين أهلية وجوب أهلية أداء أهلية الوجوب قسمها
+
+47
+00:04:18,580 --> 00:04:23,100
+العلماء إلى قسمين ممكن تلاقي يعني في بعض كتب
+
+48
+00:04:23,100 --> 00:04:29,050
+الأصول أنها تلت أقسام مافيش إشكاليةاحنا خلّينا
+
+49
+00:04:29,050 --> 00:04:34,790
+نعتمد القسمين هذه أهلية الوجوب أهلية ناقصة وأهلية
+
+50
+00:04:34,790 --> 00:04:40,510
+كاملة كما قسمها العلماء أما أهلية الأداء فقسموها
+
+51
+00:04:40,510 --> 00:04:46,670
+إلى ثلاثة أقسام أهلية أداء منعدمة أهلية أداء ناقصة
+
+52
+00:04:46,670 --> 00:04:52,210
+أهلية أداء كاملة تعالوا نشوف ونفصل في هذا الكلام
+
+53
+00:04:52,210 --> 00:04:58,010
+نيجي أول لأهلية الوجوباهلية الوجوب زي ما حكينا،
+
+54
+00:04:58,010 --> 00:05:02,710
+عندي أهلية وجوب ناقصة وعندي أهلية وجوب كاملة
+
+55
+00:05:02,710 --> 00:05:09,130
+السؤال الأن، ما معنى أهلية الوجوب؟ إيش يعني أهلية
+
+56
+00:05:09,130 --> 00:05:16,090
+وجوب؟ أهلية وجوب هي صلاحية الإنسان لثبوت الحق له
+
+57
+00:05:16,090 --> 00:05:21,790
+أو عليهاطيب كيف نثبت الحق له أو عليها؟ و ذلك
+
+58
+00:05:21,790 --> 00:05:28,090
+بمقتضى الذمة التي يولد بها كل آدمي، إذا الذمة هي
+
+59
+00:05:28,090 --> 00:05:34,990
+الوعاء، هي مكان الوجوب، وهي وصف اختص الله سبحانه
+
+60
+00:05:34,990 --> 00:05:40,910
+وتعالى به الإنسان دون غيره من الحيوانات التي فقدت
+
+61
+00:05:40,910 --> 00:05:46,600
+الذمة، إذا الذمة هذه بس موجودة لمين؟ موجودةللإنسان
+
+62
+00:05:46,600 --> 00:05:50,780
+ربنا سبحانه وتعالى اختص الإنسان بها دون سائر
+
+63
+00:05:50,780 --> 00:05:57,060
+المخلوقات إذا الذمة هي أساس هذه الأهلية و الذمة
+
+64
+00:05:57,060 --> 00:06:02,460
+تساوي أيه؟ تساوي الحياة و لا تكون الذمة للإنسان
+
+65
+00:06:02,460 --> 00:06:07,880
+إلا بتحقق الحياة تمام؟ إذا أساس أهلية الوجوب هي
+
+66
+00:06:07,880 --> 00:06:13,540
+الذمة و الذمة تساوي أيه؟ تساوي الحياةلا تكون
+
+67
+00:06:13,540 --> 00:06:20,940
+للإنسان ذمة إلا إيه؟ إلا بتحقق الحياة نيجي لمعنى
+
+68
+00:06:20,940 --> 00:06:26,540
+الذمة في الإصطلاح كما أعرفها العلماء هي أمر أو وصف
+
+69
+00:06:26,540 --> 00:06:32,500
+يصير به الإنسان أهلا للإيجاب والاستجاب أي للالذام
+
+70
+00:06:32,500 --> 00:06:38,140
+والالتزاموتثبت ذمة للإنسان بناء على العهد الذي جرع
+
+71
+00:06:38,140 --> 00:06:44,140
+بينه وبين الحق سبحانه وتعالى يوم أن أخذ عليه العهد
+
+72
+00:06:44,140 --> 00:06:47,480
+وكان
+
+73
+00:06:47,480 --> 00:06:55,000
+بهذا العهد مسئول هذه الأهلية تتحقق للإنسان و هو في
+
+74
+00:06:55,000 --> 00:07:01,660
+بطن أمه قبل أن يولدتثبت له الحقوق التي لا تحتاج
+
+75
+00:07:01,660 --> 00:07:08,360
+إلى القبول مثل الارث والوصية والوقف وتستمر معه هذه
+
+76
+00:07:08,360 --> 00:07:14,580
+الأهلية حتى الموت، إذا الإنسان الأهلية هذه أهلية
+
+77
+00:07:14,580 --> 00:07:19,930
+الوجودطبعا أهلية الوجوب تساوي الحياة أو تساوي
+
+78
+00:07:19,930 --> 00:07:25,770
+الذمة هذه الأهلية تتحقق للإنسان وهو في بطن أمه قبل
+
+79
+00:07:25,770 --> 00:07:30,170
+أن يولد تثبت له الحقوق التي لا تحتاج إلى القبول
+
+80
+00:07:30,170 --> 00:07:36,150
+وتستمر معه هذه الأهلية حتى الموت إذا كل إنسان حي
+
+81
+00:07:36,150 --> 00:07:41,390
+له أهلية وجوب كل إنسان حي له أهلية وجوب
+
+82
+00:07:44,040 --> 00:07:51,260
+أحوال أهلية الوجوب عندي حالتين الحالة الأولى أهلية
+
+83
+00:07:51,260 --> 00:07:57,380
+وجوب ناقصة والحالة التانية أهلية وجوب كاملة أهلية
+
+84
+00:07:57,380 --> 00:08:04,580
+وجوب ناقصة هي التي تؤهل من اتصف بها لثبوت الحق له
+
+85
+00:08:04,580 --> 00:08:09,280
+دون أن يكون أهلا لثبوت أي حق عليها وهذا لا يكون
+
+86
+00:08:09,280 --> 00:08:16,650
+إلا في الجنينالجنين متى تأكدت حياته في بطن أمه حتى
+
+87
+00:08:16,650 --> 00:08:23,530
+يولد حيا فهو أهل ثبوت الحقوق له من إرث ووصية
+
+88
+00:08:23,530 --> 00:08:28,630
+واستحقاق للوقف وثبوت النسب له من جهة أبي ولكن لا
+
+89
+00:08:28,630 --> 00:08:33,830
+يثبت عليه أي حق من الحقوق فلا يصح للوكيل الشراء له
+
+90
+00:08:33,830 --> 00:08:37,990
+ولا الهبة ولا الالتزام بالنفقة لقريب أو غيره لأن
+
+91
+00:08:37,990 --> 00:08:44,160
+الجنين يجمع بين صفتينالصفة .. الصفة الأولى هو تابع
+
+92
+00:08:44,160 --> 00:08:51,300
+لأمه والصفة التانية وهو معدل الاستقلال عنها عشان
+
+93
+00:08:51,300 --> 00:08:56,780
+هيك الآن بستحضرنا سؤال إيش السبب اللي خلانا نعتبر
+
+94
+00:08:56,780 --> 00:09:01,620
+أهلية الوجوب هذه للجنين ناقظة؟ ليش ما تعتبرها
+
+95
+00:09:01,620 --> 00:09:05,740
+كاملة؟ مش هي .. قلتلي إنه حي في بطن أمه و ال .. و
+
+96
+00:09:05,740 --> 00:09:11,670
+الأساسي الأهلية الوجوب ال .. الحياءلكن لماذا اعتبر
+
+97
+00:09:11,670 --> 00:09:17,370
+المشرع هذه الأهلية ناقصة؟ عندي طبعا الجواب سببين
+
+98
+00:09:17,370 --> 00:09:22,990
+السبب الأول التابعية لأمه تابع لأمه التابعية في
+
+99
+00:09:22,990 --> 00:09:29,550
+ارتباطه بامه حسا لتحركه بتحركها وقراره بقرارها فهو
+
+100
+00:09:29,550 --> 00:09:33,190
+جزء منها كاليد والرجل وسائر الأعضاء وارتباطه بيها
+
+101
+00:09:33,190 --> 00:09:39,330
+حكما أيضا اذ يباعوا ببيعها ويعتقوا بعتقهاالسبب
+
+102
+00:09:39,330 --> 00:09:43,610
+الأول في النقصان تابع لإمه، عشان هيك اعتبرنا ناقص
+
+103
+00:09:43,610 --> 00:09:51,370
+لإن هو تابع لإمه في كل حركة بتتحركها أما السبب
+
+104
+00:09:51,370 --> 00:09:57,370
+الثاني هو الاستقلال عنها، طب كيف؟ الجنين الآن
+
+105
+00:09:57,370 --> 00:10:02,470
+منفرد عن الأم بحياته، بتنفس لحاله، في عنده رئة و
+
+106
+00:10:02,470 --> 00:10:09,080
+في عنده قلب، وله كيان ومقومات إنسانيةوهو بذلك معدل
+
+107
+00:10:09,080 --> 00:10:14,820
+الانفصال عن أمه ليصير ذاتا مستقلة عشان هيك
+
+108
+00:10:14,820 --> 00:10:21,340
+التباعيته هذه ليست مطلقة بل هي تباعية جزئية، لذا
+
+109
+00:10:21,340 --> 00:10:26,800
+التباعية مدتها تسع شهور و بعد هيك هينفصل عن أمه و
+
+110
+00:10:26,800 --> 00:10:31,920
+يصير إنسان كامل عشان هيك اعتبر المشرع هذه أهلية
+
+111
+00:10:31,920 --> 00:10:36,940
+الوجوب أهلية ناقصةلكن لو إجوا حد سألكوا يعني إيش
+
+112
+00:10:36,940 --> 00:10:42,420
+ناقصة؟ يعني نص بتنفس و نص لأ؟ نص حي و نص لأ؟
+
+113
+00:10:42,420 --> 00:10:47,180
+بنقوله لأ، دير بالة كإن إحنا سببين، هو صح تابع
+
+114
+00:10:47,180 --> 00:10:53,810
+لأمهمعدل الاستقلال عنها عشان هيك المشرع ما اعتبرش
+
+115
+00:10:53,810 --> 00:10:58,830
+التباعية هذه تباعية كاملة بل اعتبرها تباعية جزئية
+
+116
+00:10:58,830 --> 00:11:04,910
+فكانت هذه التباعية مستدعية لعدم ثبوت الحق له سواء
+
+117
+00:11:04,910 --> 00:11:10,310
+كان الحق ارثا او وقفا او وصية لكن المشرع الحكيم
+
+118
+00:11:10,310 --> 00:11:15,970
+نظر في صفة الاستقلالوأنه نفس حية ذات طبيعة خاصة
+
+119
+00:11:15,970 --> 00:11:23,630
+فأعطاه بالنظر لإستقلاله ومنعه لتبعيته تمام؟ إذا
+
+120
+00:11:23,630 --> 00:11:29,070
+لماذا اعتبرنا أهلية الوجوب هذه ناقصة؟ في عندي
+
+121
+00:11:29,070 --> 00:11:33,390
+سببين أنه أول حاجة تهبه لأمه والسبب التاني أنه
+
+122
+00:11:33,390 --> 00:11:39,590
+معدل الاستقلال عنها فكانت تبعيته لأمه مستدعية عدم
+
+123
+00:11:39,590 --> 00:11:46,200
+ثبوت الحق له لكن استعدادهوصفة الاستقلال اللى فيه
+
+124
+00:11:46,200 --> 00:11:53,060
+نظرة لها المشرع نظرة حكمة وأنه نفس حية ذات طبيعة
+
+125
+00:11:53,060 --> 00:12:00,420
+خاصة فأعطاه بالنظر للاستقلال ومنعه بالنظر للتبعية
+
+126
+00:12:00,420 --> 00:12:06,780
+فكان هذا النقص نأتي الآن للحديث عن أهلية الوجوب
+
+127
+00:12:06,780 --> 00:12:10,320
+الكاملة أهلية الوجوب الكاملة زى ما أنتوا شايفين
+
+128
+00:12:10,700 --> 00:12:17,640
+تثبت للإنسان منذ ولادته دون أن تفارقوا في جميع
+
+129
+00:12:17,640 --> 00:12:24,080
+أدوار حياته فيصلح الانسان لتلقى الحقوق والالتزام
+
+130
+00:12:24,080 --> 00:12:29,200
+بالواجبات وخدوا بالكم من هذه العبارة المهمة اللي
+
+131
+00:12:29,200 --> 00:12:34,880
+ركزنا عليها في المحاضرة ولا يوجد إنسان فاقد لهذه
+
+132
+00:12:34,880 --> 00:12:41,080
+الأهلية فأهلية الوجوب هيما كان المتصف بها صالحا
+
+133
+00:12:41,080 --> 00:12:46,480
+لثبوت الحق له وعليه بحيث تكون ذمته مطالبة
+
+134
+00:12:46,480 --> 00:12:53,880
+بالالتزامات المالية أساس الأهلية الحياة كل إنسان
+
+135
+00:12:53,880 --> 00:13:06,140
+حي يستحق أهلية الوجوب الكاملة بهذه
+
+136
+00:13:06,140 --> 00:13:10,420
+الأهليةيصلح الإنسان أن يتلقى الحقوق وأن يلتزم
+
+137
+00:13:10,420 --> 00:13:14,960
+بالواجبات وأهلية الوجوب الكاملة تتحقق لكل مولود
+
+138
+00:13:14,960 --> 00:13:19,800
+سواء كان هذا المولود مميزا ام لا ويثبت لكل مولود
+
+139
+00:13:19,800 --> 00:13:24,780
+ما يليه خلينا نستعرض النقاط هذه بدون تفصيل لأن
+
+140
+00:13:24,780 --> 00:13:30,920
+تفصيلها هيكون جاي في عوارض الأهلية صلاحيته اللي هو
+
+141
+00:13:30,920 --> 00:13:37,170
+طبعا الإنسانصلاحية ذمته للالتزام بما يقوم به وليه
+
+142
+00:13:37,170 --> 00:13:43,010
+اللي هو احنا بنتحدث عن المولود قبل
+
+143
+00:13:43,010 --> 00:13:48,570
+البلوغ صلاحية ذمته للالتزام بما يقوم به وليه من
+
+144
+00:13:48,570 --> 00:13:54,250
+تصرفات مع التقييد بها ان بلغها الرجل لأنها وقعت
+
+145
+00:13:54,250 --> 00:13:58,930
+باسمه وفي الحدود التي أجازهاحكينا احنا بدناش تفصيل
+
+146
+00:13:58,930 --> 00:14:03,610
+كتير في هذا الكلام لسه هنشرحه بالتفصيل ان شاء الله
+
+147
+00:14:03,610 --> 00:14:09,550
+عند الحديث عن عوارض الأهلين يثبت في مالهم اللي هم
+
+148
+00:14:09,550 --> 00:14:14,290
+الصغار يعني كل ما فيه مقنع كصدقة الفطر اكتفاء
+
+149
+00:14:14,290 --> 00:14:19,180
+بالاهلية القاسرة بواسطة الوليأيضًا ضمان ما يطلفه
+
+150
+00:14:19,180 --> 00:14:23,700
+القاصر من أموال لصلاحية ذمته لثبوت كل واجب مالي
+
+151
+00:14:23,700 --> 00:14:28,580
+عليها لأن المقصود منه ضمان التلف ويقوم بأدائه وليه
+
+152
+00:14:28,580 --> 00:14:34,480
+نيابة عنه وانتوا بتسمعوا يمكن عندنا بتسمعوا
+
+153
+00:14:34,480 --> 00:14:39,160
+جداتكوا أجدادكوا إيش بيحكوا لصغير وراه كبير يعني
+
+154
+00:14:39,160 --> 00:14:44,880
+إذا أطلف الصغير شيئًا تمام أو تعدى على حقوق
+
+155
+00:14:44,880 --> 00:14:50,120
+الآخرينمش هو اللي بيتحسب و ليه اللي بيتحسب و يضمن
+
+156
+00:14:50,120 --> 00:14:57,120
+المتلف من ما يتلفه القاصر زي ما حكينا في تفصيل لسه
+
+157
+00:14:57,120 --> 00:15:02,420
+على هذا الكلام عند الحديث عن عوارض الأهلية نقف
+
+158
+00:15:02,420 --> 00:15:07,460
+الآن عند أهلية الأداء لتكون في المحاضرة القادمة
+
+159
+00:15:07,460 --> 00:15:13,300
+بإذن الله و السلام عليكم و رحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/dNcz-Gf_p5I.srt b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/dNcz-Gf_p5I.srt
new file mode 100644
index 0000000000000000000000000000000000000000..56c18d8fe234bec5bb64cb08ba12129e9f65b7f5
--- /dev/null
+++ b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/dNcz-Gf_p5I.srt
@@ -0,0 +1,879 @@
+1
+00:00:08,400 --> 00:00:11,140
+أعوذ بالله من الشيطان الرجيم بسم الله الرحمن
+
+2
+00:00:11,140 --> 00:00:15,580
+الرحيم إن الحمد لله نحمده ونستعينه ونستغفره ونعوذ 
+
+3
+00:00:15,580 --> 00:00:19,680
+بالله من شرور أنفسنا وسيئات أعمالنا ما يهدي الله 
+
+4
+00:00:19,680 --> 00:00:24,140
+فلا مضل له وما يضل فلا هادي له وأشهد أن لا إله إلا 
+
+5
+00:00:24,140 --> 00:00:29,240
+الله وأشهد أن محمد عبده ورسوله وبعد بداية أخوات 
+
+6
+00:00:29,240 --> 00:00:33,940
+الحبيبات وبنات الطيبات أحييكم بتحية طيبة مباركة من 
+
+7
+00:00:33,940 --> 00:00:38,430
+عند الله السلام عليكم ورحمة الله وبركاته اليوم إن 
+
+8
+00:00:38,430 --> 00:00:43,330
+شاء الله سنستكمل الحديث عن العوارض كنا قد انتهينا 
+
+9
+00:00:43,330 --> 00:00:48,930
+من العوارض السماوية وقلنا أننا قدمنا العوارض 
+
+10
+00:00:48,930 --> 00:00:54,370
+السماوية لأنها ليست بيد الإنسان وتركنا أو أخرنا 
+
+11
+00:00:54,370 --> 00:00:58,130
+العوارض المكتسبة اليوم إن شاء الله هنتكلم عن هذه 
+
+12
+00:00:58,130 --> 00:01:02,350
+العوارض، صرتوا أنتم كلكم عارفين أنه كل عارض من 
+
+13
+00:01:02,350 --> 00:01:09,810
+هذه العوارض يجب أن نعرف تعريفه ونأخذ أمثلة عليه وعلى 
+
+14
+00:01:09,810 --> 00:01:15,750
+ماذا يؤثر في أهلية الإنسان أما بالنسبة لتعريف 
+
+15
+00:01:15,750 --> 00:01:21,550
+العوارض المكتسبة هي ما تكون من فعل الإنسان بقدرة 
+
+16
+00:01:21,550 --> 00:01:26,670
+منه اختيارًا هذا طبعًا إيش؟ بخلاف عن قضية العوارض 
+
+17
+00:01:26,670 --> 00:01:31,830
+السماوية التي لا يكون للإنسان فيها اختيار ولا قدرة 
+
+18
+00:01:32,090 --> 00:01:37,550
+أما هذه العوارض المكتسبة هنتكلم عنها بالتفصيل وهي 
+
+19
+00:01:37,550 --> 00:01:44,510
+الجهل والسكر والهزل والسفه والخطأ والسفر والإكراه 
+
+20
+00:01:44,510 --> 00:01:50,310
+طبعًا هذه العوارض إما أن تكون واقعة من الإنسان نفسه 
+
+21
+00:01:50,310 --> 00:01:55,470
+هو الذي عملها أو من غيره عليه من الإنسان نفسه 
+
+22
+00:01:55,470 --> 00:02:00,180
+كالجهل والسكر والهزل والسفه والخطأ والسفر أما 
+
+23
+00:02:00,180 --> 00:02:06,060
+العوارض المكتسبة الواقعة من غيره عليها زي .. زي 
+
+24
+00:02:06,060 --> 00:02:11,560
+الإكراه فقط نجي لموضوع السكر طبعًا بالنسبة ليه 
+
+25
+00:02:11,560 --> 00:02:17,620
+السكر علماء الأصول اعتبروا من العوارض المكتسبة وإن 
+
+26
+00:02:17,620 --> 00:02:23,060
+لم يكن حصوله في مقدور الإنسان كيف يعني؟ يعني حالة 
+
+27
+00:02:23,060 --> 00:02:26,160
+السكر هذه مش أنا الذي صنعتها يعني ممكن الإنسان 
+
+28
+00:02:26,160 --> 00:02:31,760
+يشرب ويسكر ممكن لأ هذا أثر يعني ليس بيد الإنسان، 
+
+29
+00:02:31,760 --> 00:02:39,560
+لِيش؟ لأن السكر شرب ما يُذكر بفعل المرء اختيارًا، 
+
+30
+00:02:39,560 --> 00:02:47,160
+لكنه قصد السكر، عشان هيك أُضيف لفعله نظرًا إلى كل من 
+
+31
+00:02:47,160 --> 00:02:52,860
+السبب والهدف طبعًا السكر حرام بالإجماع عروض النصوص 
+
+32
+00:02:52,860 --> 00:02:58,430
+القاطعة بتحريمه من الكتاب أو السنة موضوعنا هو تعريف 
+
+33
+00:02:58,430 --> 00:03:04,930
+السكر السكر هو حالة تعرض لها الإنسان من إمتلاء دماغه 
+
+34
+00:03:04,930 --> 00:03:10,610
+من الأبخرة المتصاعدة إليه فيتعطل معه عقله المميز 
+
+35
+00:03:10,610 --> 00:03:18,350
+بين الأمور الحسنة والقبيحة نجي الآن لتأثير السكر 
+
+36
+00:03:18,350 --> 00:03:23,390
+وهذا مهم كثير في موضوعنا أساسًا احنا كل العوارض عشان 
+
+37
+00:03:23,390 --> 00:03:27,090
+نشوف أثر هذه حديثنا عن العوارض عشان نشوف أثر هذه 
+
+38
+00:03:27,090 --> 00:03:32,210
+العوارض على أهلية الإنسان بالنسبة لها تأثير السكر 
+
+39
+00:03:32,210 --> 00:03:37,530
+اختلف العلماء في تأثير السكر على عقل الإنسان بين 
+
+40
+00:03:37,530 --> 00:03:41,970
+الإزالة والسكر المؤقت الذي ينتهي بإفاقة السكران 
+
+41
+00:03:41,970 --> 00:03:48,170
+فإذا زالت السكر رجع العقل لحالته الطبيعية التي كان 
+
+42
+00:03:48,170 --> 00:03:53,780
+عليها قبل تناول المسكر خذوا بالكم خلاف العلماء هل 
+
+43
+00:03:53,780 --> 00:03:59,100
+أزيل العقل تمامًا أو ستر العقل مؤقتًا ويعود بعد 
+
+44
+00:03:59,100 --> 00:04:05,280
+انتهاء حالة السكر؟ تمام؟ هذه الرأي الرأي الثاني 
+
+45
+00:04:05,280 --> 00:04:11,200
+وهو الراجح الرأي الثاني هو الراجح ليش؟ لأن السكر 
+
+46
+00:04:11,200 --> 00:04:17,210
+لا يزيل العقل حقيقة العقل موجود ولكنه يُفوت قدرة 
+
+47
+00:04:17,210 --> 00:04:22,930
+السكران على إيش؟ على فهم الخطاب و هنا انتبهوا 
+
+48
+00:04:22,930 --> 00:04:28,010
+معايا كويس يفرقوا بين السكر المباح والسكر بسبب 
+
+49
+00:04:28,010 --> 00:04:33,070
+المعصية كلكم بتتطلعوا في بعض هلّق؟ سكر مباح؟ إيه؟ 
+
+50
+00:04:33,070 --> 00:04:36,290
+هو في حاجة اسمها سكر مباح؟ اه خلّي بالك معايا كيف 
+
+51
+00:04:36,290 --> 00:04:44,850
+سيكون سكر بطريق المباح كسكر المضطر يعني الذي اضطر 
+
+52
+00:04:44,850 --> 00:04:50,990
+إلى شرب الخمر وسكر المكره على شرب الخمر وأيضًا 
+
+53
+00:04:50,990 --> 00:04:55,650
+السكر بدواء كالبنج الأفيون يعني واحد مريض بياخد 
+
+54
+00:04:55,650 --> 00:05:03,490
+دواء و سكر فإن حكمه هنا يكون كالْإغماء فيمنع من صحة 
+
+55
+00:05:03,490 --> 00:05:08,270
+جميع التصرفات لأن ذلك ليس من جنس الهَوَى فصار من 
+
+56
+00:05:08,270 --> 00:05:14,290
+أقسام المرض فلا يطالب بالتكاليف حال غيبوبته ويطالب 
+
+57
+00:05:14,290 --> 00:05:20,990
+بالقضاء بعد الإفاقة إذا السكر الباح المشرع يعني 
+
+58
+00:05:20,990 --> 00:05:27,100
+عمل له رعاية خاصة لأنه ليس له يد فيه هذا السكر عشان 
+
+59
+00:05:27,100 --> 00:05:32,060
+هيك سمّيناه سكر قبح يعني واحد بدهم يعملوله عملية 
+
+60
+00:05:32,060 --> 00:05:38,400
+وبنجوه سكر واحد اضطر غصًّا بلقمة واضطر لشرب ما وجدش 
+
+61
+00:05:38,400 --> 00:05:44,920
+لخمر واضطر إلى شرب هذا المسكر والمكره كذلك أما هنا 
+
+62
+00:05:44,920 --> 00:05:51,330
+الموضوع عاد أن سكرته بسبب ما هو معصية فيجعل العقل في 
+
+63
+00:05:51,330 --> 00:05:57,150
+حكم الموجود زجرًا له وعقابًا على ذنبه الذي نهاه 
+
+64
+00:05:57,150 --> 00:06:03,770
+عنه الشارع الحكيم هنا الحكم يلزم السكران بهذه 
+
+65
+00:06:03,770 --> 00:06:10,570
+الحالة بكل الأحكام وتصحّ أفعاله كلها، كل العبارات 
+
+66
+00:06:10,570 --> 00:06:13,550
+التي ينطقها، الطلاق والاعتاق والبيع والشراء و 
+
+67
+00:06:13,550 --> 00:06:18,290
+الإقرارات والزواج وسائل التصرفات كلها يُلزم فيها، 
+
+68
+00:06:18,290 --> 00:06:22,990
+حتى إذا ارتكب جريمة يُعاقب عليها، فإذا قتل يجب عليه 
+
+69
+00:06:22,990 --> 00:06:27,630
+القصاص وإذا سرقة أو قذف أو زنى يقع عليه الحد بعد 
+
+70
+00:06:27,630 --> 00:06:34,920
+الصحة نأتي للعرض الثاني وهو الهزل، إيش الهزل يعني؟ 
+
+71
+00:06:34,920 --> 00:06:39,400
+الهزل الذي هو المزح، وهو نطق الإنسان بالكلام 
+
+72
+00:06:39,400 --> 00:06:44,740
+راضياً، فاهماً، لمعناه دون أن يقصد به الحقيقة 
+
+73
+00:06:44,740 --> 00:06:51,340
+ولا المجاز، تمام؟ يعني واحد بمزح مع الثاني، بمزح، 
+
+74
+00:06:51,340 --> 00:06:57,780
+فاهم الذي يحكيه وفاهم معناه لكن قصده ونيّته 
+
+75
+00:06:57,780 --> 00:07:03,980
+ليست لا حقيقة ولا إيش ولا مجازًا .. مزح زي ما بقولوا 
+
+76
+00:07:03,980 --> 00:07:09,560
+خذوا بالكم معايا بعد الآن شرط الهزل أن يكون منطوقًا 
+
+77
+00:07:09,560 --> 00:07:14,900
+به صراحةً يعني أنا بمزح معك بعتك هذا البيت استنى 
+
+78
+00:07:14,900 --> 00:07:19,380
+استنى بمزح معاك ما فيش لا بيع ولا شراء يعني هي نطقة 
+
+79
+00:07:19,880 --> 00:07:26,280
+لكن لو حكى هو قعد بدحك وحكى بعتك هذا البيت وهو 
+
+80
+00:07:26,280 --> 00:07:32,240
+حكى قابلت خلاص انتهى الموضوع لأن لم يصرح أن هذا ال
+
+81
+00:07:32,240 --> 00:07:37,960
+... ال ... البيع هازل تمام؟ لم يصرح عشان هيك بنقول 
+
+82
+00:07:37,960 --> 00:07:42,100
+شرط الهازل أن يكون منطوقًا به صراحةً بمزح معاك كما 
+
+83
+00:07:42,100 --> 00:07:46,580
+إذا قيل أتصرف في هذا العمل أو أبيعه أو أشتريه 
+
+84
+00:07:46,580 --> 00:07:52,390
+هازلاً ومعنى هذا أن دلالة الحال غير كافية فيه كيف 
+
+85
+00:07:52,390 --> 00:07:56,330
+يعني؟ دلالة الحال يعني إذا كان مثلًا في حالة ضحك أو 
+
+86
+00:07:56,330 --> 00:08:03,370
+حالة يعني أن الحالة تبعته بتصرفه وشكله أنه بمزح، 
+
+87
+00:08:03,370 --> 00:08:07,750
+لأ ما لناش دعوة احنا في دلالة الحال إلينا في النطق 
+
+88
+00:08:07,750 --> 00:08:13,230
+الصريح تمام؟ كمان مرة؟ وللأهمية شرط الهزل أن يكون 
+
+89
+00:08:13,230 --> 00:08:19,090
+منطوقًا به صراحةً ولا تكفي فيه دلالة الحال حكم الهزل 
+
+90
+00:08:19,090 --> 00:08:24,790
+يعدّ الهزل منافع لاختيار الحكم والرضا به، ولكنه غير 
+
+91
+00:08:24,790 --> 00:08:30,050
+منافع لأهلية المرء ولا لاختيار المباشرة أو الرضا 
+
+92
+00:08:30,790 --> 00:08:36,310
+تعالوا نشوف نقطة مهمة تتعلق بالهازل بالنسبة لتصرفات 
+
+93
+00:08:36,310 --> 00:08:41,810
+الهازل حسب الرضا والاختيار هنقسمها لثلاثة أقسام 
+
+94
+00:08:41,810 --> 00:08:47,670
+إنشاءات، إخبارات، اعتقادات تعالوا نشوف تصرفات 
+
+95
+00:08:47,670 --> 00:08:57,910
+الهازل في الإنشاءات، في الإخبارات، في الاعتقادات 
+
+96
+00:09:01,810 --> 00:09:07,690
+بالنسبة للإنشاءات، إيش يعني إنشاءات؟ هي التصرفات 
+
+97
+00:09:07,690 --> 00:09:13,290
+التي يترتب عليها إحداث حكم شرعي وهذه الإنشاءات 
+
+98
+00:09:13,290 --> 00:09:20,190
+قسمين، قسم لا يؤثر فيه الهزل وقسم يؤثر فيه الهزل 
+
+99
+00:09:20,190 --> 00:09:27,650
+القسم الذي جعله المشرع لا يؤثر فيه الهزل لا ينبغي 
+
+100
+00:09:27,650 --> 00:09:33,910
+الهزل في ما فيه حق لله تعالى مثل النكاح والطلاق 
+
+101
+00:09:33,910 --> 00:09:38,710
+والاعتاق عندنا نص عن النبي صلى الله عليه وسلم 
+
+102
+00:09:38,710 --> 00:09:44,610
+ثلاثة جدّهن جدّ وهزلهن جدّ يعني إذا مزحت في طلاق 
+
+103
+00:09:44,610 --> 00:09:51,280
+ومزحت في نكاح في اعتاق كل واقع يعني هان الهزل لو 
+
+104
+00:09:51,280 --> 00:09:56,440
+واحد طلق زوجته وقال لها أنه مازحًا أو هازلة، وقع 
+
+105
+00:09:56,440 --> 00:10:01,800
+الطلاق، ليش؟ لأنه ما ينفعش بالمرة يقرب على حق من 
+
+106
+00:10:01,800 --> 00:10:06,060
+حقوق الله تعالى، لا ينبغي الهزل في ما فيه حق لله 
+
+107
+00:10:06,060 --> 00:10:10,880
+تعالى أما القسم الذي يؤثر فيه الهزل، الانتفاع، 
+
+108
+00:10:10,880 --> 00:10:15,100
+أساس الالتزام، وهو القصد والإرادة، زي البيع 
+
+109
+00:10:15,100 --> 00:10:20,810
+والإجارة أما الإخبارات وهي ما يُقصد بها بيان الواقع 
+
+110
+00:10:20,810 --> 00:10:25,590
+الهزل طبعًا مبطل للإخبارات لأن إقرار الإنسان بها 
+
+111
+00:10:25,590 --> 00:10:30,290
+يعتمد على صحة الأخبار به والهزل منافٍ لاعتماد 
+
+112
+00:10:30,290 --> 00:10:37,550
+الصحة ولهذا فإن الآخر هازلاً يعدّ إقراره باطلًا تمام؟ 
+
+113
+00:10:37,550 --> 00:10:43,290
+أما بالنسبة للاعتقادات وهي ماذا يُقصد به بيان 
+
+114
+00:10:43,590 --> 00:10:48,890
+الواقع بل الاعتقاد والتدين به بعد اعتقاد القلب به 
+
+115
+00:10:48,890 --> 00:10:53,490
+طبعًا الهزل بالردة كفر لأنه استخفاف بالدين لا يجيز 
+
+116
+00:10:53,490 --> 00:10:59,230
+ذلك وبهذا يعتبر مرتدًا تُطبق عليه أحكام الردة ويكون 
+
+117
+00:10:59,230 --> 00:11:04,550
+ارتداده بالهزل نفسه بصرف النظر عما قاله في الهزل 
+
+118
+00:11:04,550 --> 00:11:07,650
+تمام؟
+
+119
+00:11:07,650 --> 00:11:18,100
+الآن ننطلق إلى عارض الثالث وهو السفه السفه طبعًا هو 
+
+120
+00:11:18,100 --> 00:11:25,580
+تصرفات الإنسان في أمواله تصرفًا مخالفًا لمقتضى العقل 
+
+121
+00:11:25,580 --> 00:11:29,880
+السليم وقواعد الشرع الحكيم مع وجود العقل حقيقة 
+
+122
+00:11:29,880 --> 00:11:36,480
+للسفيه السفيه موجود عقل لكن تصرفاته مخالفة لمقتضى 
+
+123
+00:11:36,480 --> 00:11:40,660
+العقل السليم تحس بتقولي هذا مش طبيعي في حاجة لكن 
+
+124
+00:11:40,660 --> 00:11:49,190
+السفه لا يوجب خللًا في الأهلية ولا يمنع شيئًا من 
+
+125
+00:11:49,190 --> 00:11:54,750
+أحكام الشرع ولا يوجب وضع الخطاب بحال رفع الخطاب 
+
+126
+00:11:54,750 --> 00:11:59,870
+يعني فيبقى السفيه أهلا لمباشرة التصرفات ومطالبا
+
+127
+00:11:59,870 --> 00:12:05,610
+بأداء العبادات، أفلام بتقول هذا سفيه يعني عقله 
+
+128
+00:12:05,610 --> 00:12:12,340
+موجود وبتحس بس في عنده يعني شوية خلل، بتقول هذا
+
+129
+00:12:12,340 --> 00:12:18,920
+بحساه مش طبيعي، لكن هذا السفه في الشرع لا يوجب 
+
+130
+00:12:18,920 --> 00:12:22,440
+خللا في الأهلية ولا يمنع شيئا من أحكام الشرع ولا
+
+131
+00:12:22,440 --> 00:12:26,980
+يوجب وضع الخطاب، في حال يعني بطلب بالصلاة ومطلب 
+
+132
+00:12:26,980 --> 00:12:31,900
+بالذكاء، ويبقى أهلا لمباشرة التصرفات زي البيع
+
+133
+00:12:31,900 --> 00:12:37,220
+والشراء وغيره ومطالبا بأداء كل العبادات، وقد أجمع
+
+134
+00:12:37,220 --> 00:12:42,660
+العلماء على أن الصبي إذا بلغ سفيها يمنع ماله لقوله
+
+135
+00:12:42,660 --> 00:12:47,180
+تعالى ولا تؤتوا السفهاء أموالكم التي جعل الله لكم
+
+136
+00:12:47,180 --> 00:12:51,540
+قيمة، فإن أصبح راشدا فيسقط حينئذ منع المال عنه
+
+137
+00:12:51,540 --> 00:12:57,000
+لقوله تعالى فانقنزتم منهم رشدا فادفعوا إليهم
+
+138
+00:12:57,000 --> 00:13:02,420
+أموالهم، إلا عند الحنفية فقد أفادوا أن مدة الحجر
+
+139
+00:13:02,420 --> 00:13:07,500
+تنتهي عند خمسة وعشرين سنة وإن لازمه الصفة بعد ذلك
+
+140
+00:13:07,500 --> 00:13:16,240
+هو صفيه يعني يمنع من التصرف في أمواله تماما بعد
+
+141
+00:13:16,240 --> 00:13:20,400
+الحجر عليه، يعني تصرفاته هو بيكون أهل لهذه
+
+142
+00:13:20,400 --> 00:13:27,180
+التصرفات، لكن بعد الحجر عليه يمنع من هذه التصرفات
+
+143
+00:13:27,180 --> 00:13:36,380
+تصبح تصرفاته غير معتبرة شرعا، نيجي الآن للخطأ، الخطأ
+
+144
+00:13:37,150 --> 00:13:42,470
+هو وقوع شيء من المكلف على غير إيرادته، لتركه 
+
+145
+00:13:42,470 --> 00:13:48,030
+التثبت عنده، مباشرة أمر مقصود سوى، يعني واحد عمال
+
+146
+00:13:48,030 --> 00:13:53,770
+بصيد، وهو بصيد مع برودة الصيد، نشن هيك على سطح
+
+147
+00:13:53,770 --> 00:13:58,570
+الجران في شاي فعصور هو، لكن للأسف طلع إيش رأس
+
+148
+00:13:58,570 --> 00:14:04,120
+جارته، راح طخل على أساس هو إيش قصده نيته يصيدوا
+
+149
+00:14:04,120 --> 00:14:10,140
+العصور، لكن اجت خطأ في الجار، إذا الخطأ هو وقوع
+
+150
+00:14:10,140 --> 00:14:14,140
+الشئ من المكلف على غير إيرادته، يعني هو مش قاصد
+
+151
+00:14:14,140 --> 00:14:20,820
+يقتل، قاصد الصيد، لكنه يعني ألزمنا إحنا وهنعاقبه
+
+152
+00:14:20,820 --> 00:14:25,360
+على أساس إنه ترك التثبت عند المباشرة، يعني إنت
+
+153
+00:14:25,360 --> 00:14:29,940
+بتصيد ماشي، بتشوف الصيادين حاملينه دور، لازم تقرب،
+
+154
+00:14:29,940 --> 00:14:34,670
+تشوف إيش اللي بدك تصيده هذا، تمام؟ عشان هيك بنقول
+
+155
+00:14:34,670 --> 00:14:38,310
+وقوع الشيء من المُكلَف على غير إيرادته لأنه
+
+156
+00:14:38,310 --> 00:14:43,550
+إرادته الصيد، لتركه التثبت عنده، مباشرة أمر مقصود
+
+157
+00:14:43,550 --> 00:14:50,030
+سوى، يعني هو قاصد صيد فقط وقع قتل، الخطأ لا ينافي
+
+158
+00:14:51,030 --> 00:14:55,850
+أهليتي الوجوب والأداء في حق المكلف لأنه لا يخل
+
+159
+00:14:55,850 --> 00:15:00,190
+بأسس الأهلية وقواعدها وهي الحياة والعقل والتمييز
+
+160
+00:15:00,190 --> 00:15:05,770
+كما عرفنا سابقا، ولذلك لم يسقط عن المكلف أي واجب من 
+
+161
+00:15:05,770 --> 00:15:07,630
+الواجبات الشرعية
+
+162
+00:15:09,680 --> 00:15:13,800
+كل هذا الكلام اللي حكينا بدنا نجاوب الآن على
+
+163
+00:15:13,800 --> 00:15:18,920
+السؤال اللي بيطرح نفسه، ما سبب جعل الخطأ عارضا مكتسبا
+
+164
+00:15:18,920 --> 00:15:24,340
+وإذا أنت قدرت إن هذا الإنسان ما كانش قاصد وأخطأ طب
+
+165
+00:15:24,340 --> 00:15:30,260
+ليش جعلتيه لي عارض من عوارض المكتسبة؟ طب جعل الخطأ
+
+166
+00:15:30,260 --> 00:15:36,560
+عارضا مكتسبا وذلك لأنه يقع نتيجة لعدم الاحتياط والـ
+
+167
+00:15:36,560 --> 00:15:44,660
+احتراز، تمام؟ ومثل ذلك صائم أراد أن يتمضمض في وضوء
+
+168
+00:15:44,660 --> 00:15:50,320
+فدخل الماء إلى جوفه، رامن صائد يعني صياد أصاب
+
+169
+00:15:50,320 --> 00:15:57,460
+إنسانا ظن أنه صيد فقتله، فكل من المتوضئ والمصيب لم
+
+170
+00:15:57,460 --> 00:16:02,700
+يقصد ما وقع منه، المتوضئ قصد الوضوء، فراح شرب ميه
+
+171
+00:16:02,700 --> 00:16:08,060
+ما كانش يقصد أنه يشرب ميه، والصائد أيضا ما كانش يقصد
+
+172
+00:16:08,060 --> 00:16:12,780
+القتل وإنما الصيد، الأول زي ما حكينا قصد وضوء فقط
+
+173
+00:16:12,780 --> 00:16:18,080
+لإيصال الماء لجوفه، والثاني قصد الصيد ولم يرد إصابة 
+
+174
+00:16:18,080 --> 00:16:24,280
+إنسان، طيب خلينا نشوف الحكاية اللي بتتعلق بالخطأ
+
+175
+00:16:24,280 --> 00:16:32,140
+الخطأ حكينا إنه شيء غير متعمد، والأصل في الشريعة عدم
+
+176
+00:16:32,140 --> 00:16:36,580
+مسئولية الفاعل خطأ، لأن المسئولية إنما تكون على فعل
+
+177
+00:16:36,580 --> 00:16:42,360
+حرمه الشارع مع الإتيان به عمدا أو فعل حرمه الشارع 
+
+178
+00:16:42,360 --> 00:16:49,200
+مع الإتيان به عمدا، يعني المسئولية متى
+
+179
+00:16:49,200 --> 00:16:55,140
+تقع على الإنسان عندما يفعل ما حرمه الله، وهذا الفعل
+
+180
+00:16:55,140 --> 00:17:02,570
+الذي فعله المحرم، أتى به أو فعله عمدا، قال تعالى
+
+181
+00:17:02,570 --> 00:17:07,910
+وليس عليكم جناح في ما أخطأتم به، ولكن ما تعمدت
+
+182
+00:17:07,910 --> 00:17:14,330
+قلوبكم، ولكن هنا استثنى العقاب على الخطأ، ومن ذلك وما
+
+183
+00:17:14,330 --> 00:17:19,330
+كان لمؤمن أن يقتل مؤمنا إلا خطأ، ومن قتل مؤمنا خطأ
+
+184
+00:17:19,330 --> 00:17:25,510
+فتحرير رقبة مؤمنة ودية مسلمة إلى أهله، سبب هذا
+
+185
+00:17:25,510 --> 00:17:31,850
+الاستثناء أن الخطأ فيه جناية من جهة أن المخطئ لم
+
+186
+00:17:31,850 --> 00:17:38,630
+يتثبت زي ما حكينافأخذ، فأخذ من هذه الناحية، ولذلك لم
+
+187
+00:17:38,630 --> 00:17:43,050
+تكتر العقوبة من الخطأ بمقدار الجريمة ذاتها وإنما
+
+188
+00:17:43,050 --> 00:17:50,190
+بمقدار عدم التثبت المؤدي إلى حصولها، بالنسبة لحكم
+
+189
+00:17:50,190 --> 00:17:55,870
+الخطأ، الخطأ طبعا بحسب حقوق الله تعالى أو حقوق
+
+190
+00:17:55,870 --> 00:18:00,370
+العباد، في حقوق الله تعالى، فيصلح الخطأ عذرا في
+
+191
+00:18:00,370 --> 00:18:04,490
+سقوطها إذا حصلها عن اجتهاد، فإن أخطأ المجتهد في 
+
+192
+00:18:04,490 --> 00:18:09,010
+الفتوى بعد استفراء وسعه وبذل جهد في تحري الصواب،
+
+193
+00:18:09,010 --> 00:18:14,680
+لا يكون آثما، بل ويستحق أجرا وإن كان مخطئ،
+
+194
+00:18:14,680 --> 00:18:22,180
+ليش؟ لأن هذا الخطأ وقع في حقوق الله التي طبعا تصلح
+
+195
+00:18:22,180 --> 00:18:27,140
+أن تكون عذرا في سقوطها، أما في حقوق العباد فلا يصلح
+
+196
+00:18:27,140 --> 00:18:31,680
+فيها عذرا، فيجب على من أتلف مال الإنسان خطأ ضمان
+
+197
+00:18:31,680 --> 00:18:37,640
+العدوان، لأن ضمان المال لجزاء فعلي
+
+198
+00:18:40,550 --> 00:18:45,390
+أما بالنسبة لحكم الخطأ من ناحية رفع الإثم في
+
+199
+00:18:45,390 --> 00:18:50,470
+الآخرة، فقد اتفق الفقهاء على رفع الخطأ للإثم في
+
+200
+00:18:50,470 --> 00:18:54,310
+الحياة الآخرة لقوله صلى الله عليه وسلم إن الله
+
+201
+00:18:54,310 --> 00:18:58,830
+تجاوز عن أمتي الخطأ والنسيان وما استكرهوا عليه، لأن
+
+202
+00:18:58,830 --> 00:19:04,210
+المقصود رفع إثم الخطأ والنسيان، ولا يعقل رفع النسيان 
+
+203
+00:19:04,210 --> 00:19:09,070
+والخطأ الذي يؤخذ من ظاهر العبارة، لأن رفع العمل
+
+204
+00:19:09,070 --> 00:19:13,990
+بعد وقوعه لا يمكن، ولأن ذلك واقع من الأمة، ولابد
+
+205
+00:19:13,990 --> 00:19:18,910
+لصحة الكلام وصدقه من تقدير ما يقتضيه صدقه وصحته
+
+206
+00:19:18,910 --> 00:19:24,890
+وهو الرفع، وهو المسمى بدلالة الاقتضاء عند علماء
+
+207
+00:19:24,890 --> 00:19:29,810
+الأصول، ولقوله تعالى ربنا لا تؤاخذنا إن نسينا أو
+
+208
+00:19:29,810 --> 00:19:38,270
+أخطأنا، كيف يعني؟ دلالة الاقتضاء يعني أنه لابد من 
+
+209
+00:19:38,270 --> 00:19:45,850
+تقدير في قوله مثلا إن الله تجاوز عن أمتي الخطأ
+
+210
+00:19:45,850 --> 00:19:49,930
+والنسيان، إيش المقدر هنا اللي سموه العلماء دلالة
+
+211
+00:19:49,930 --> 00:19:55,930
+الاقتضاء؟ المقدر إن الله تجاوز عن أمتي إثم الخطأ
+
+212
+00:19:55,930 --> 00:19:59,730
+والنسيان، ولم يتجاوز عن الخطأ والنسيان، لأن الخطأ
+
+213
+00:19:59,730 --> 00:20:03,610
+والنسيان خلاص وقعوا وانتهينا خلاص لا يمكن رفع، لكن
+
+214
+00:20:03,610 --> 00:20:08,650
+الذي يرفع هو الإثم، عشان يكون كلام النبي هنا صح
+
+215
+00:20:08,650 --> 00:20:12,910
+وهو المعصوم، لابد من التقدير، هذا اللي سموه علماء
+
+216
+00:20:12,910 --> 00:20:19,360
+الأصول دلالة إيه؟ دلالة الاقتضاء، نكتفي في هذه
+
+217
+00:20:19,360 --> 00:20:23,440
+المحاضرة بهذا القدر، على أن نستكمل إن شاء الله في
+
+218
+00:20:23,440 --> 00:20:30,000
+المحاضرات القادمة باقي العوارض المكتسبة، أقول قولي
+
+219
+00:20:30,000 --> 00:20:34,980
+هذا وأستغفر الله لي ولكم، سبحانك اللهم وبحمدك، وأشهد
+
+220
+00:20:34,980 --> 00:20:39,240
+أن لا إله إلا الله، وأشهد أن محمدًا عبده ورسوله 
diff --git a/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/dNcz-Gf_p5I_raw.json b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/dNcz-Gf_p5I_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..ea8ff406e9e636d2fdedaa0c0b615f5d57a07cf0
--- /dev/null
+++ b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/dNcz-Gf_p5I_raw.json
@@ -0,0 +1 @@
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{"start": 1205.43, "end": 1206.55, "word": " الإثم", "probability": 0.7205403645833334}, {"start": 1206.55, "end": 1206.93, "word": " عشان", "probability": 0.96728515625}, {"start": 1206.93, "end": 1207.19, "word": " يكون", "probability": 0.991455078125}, {"start": 1207.19, "end": 1207.51, "word": " كلام", "probability": 0.975830078125}, {"start": 1207.51, "end": 1207.79, "word": " النبي", "probability": 0.507080078125}, {"start": 1207.79, "end": 1208.35, "word": " هنان", "probability": 0.4366861979166667}, {"start": 1208.35, "end": 1208.65, "word": " صح", "probability": 0.99365234375}, {"start": 1208.65, "end": 1208.97, "word": " وهو", "probability": 0.686767578125}, {"start": 1208.97, "end": 1209.57, "word": " المعصوم", "probability": 0.908203125}, {"start": 1209.57, "end": 1210.35, "word": " لابد", "probability": 0.9044596354166666}, {"start": 1210.35, "end": 1210.67, "word": " من", "probability": 0.9931640625}, {"start": 1210.67, "end": 1211.25, "word": " التقدير", "probability": 0.86474609375}, {"start": 1211.25, "end": 1211.53, "word": " هذا", "probability": 0.87744140625}, {"start": 1211.53, "end": 1212.11, "word": " اللي", "probability": 0.96728515625}, {"start": 1212.11, "end": 1212.55, "word": " سموه", "probability": 0.7789306640625}, {"start": 1212.55, "end": 1212.91, "word": " علماء", "probability": 0.9773763020833334}, {"start": 1212.91, "end": 1213.55, "word": " الأصول", "probability": 0.9480794270833334}, {"start": 1213.55, "end": 1214.37, "word": " دلالة", "probability": 0.9779052734375}, {"start": 1214.37, "end": 1214.95, "word": " إيه؟", "probability": 0.7506103515625}, {"start": 1214.95, "end": 1215.53, "word": " دلالة", "probability": 0.99072265625}, {"start": 1215.53, "end": 1216.51, "word": " الاقتضاء", "probability": 0.706298828125}], "temperature": 1.0}, {"id": 50, "seek": 123924, "start": 1217.56, "end": 1239.24, "text": "نكتفي في هذه المحاضرة بهذا القدر على أن نستكمل ان شاء الله في المحاضرات القادمة باقي العوارض المكتسبة أقول قولي هذا وأستغفر الله لي ولكم سبحانك اللهم وبحمدك وأشهد أن لا إله إلا الله وأشهد أن محمدًا عبده ورسوله", "tokens": [1863, 4117, 2655, 41185, 8978, 29538, 9673, 5016, 46958, 25720, 39627, 15730, 25062, 3215, 2288, 15844, 14739, 8717, 14851, 24793, 1211, 16472, 13412, 16606, 21984, 8978, 9673, 5016, 46958, 2288, 9307, 25062, 18513, 46007, 4724, 995, 38436, 18863, 2407, 9640, 11242, 9673, 4117, 2655, 35457, 3660, 5551, 39648, 12174, 12610, 1829, 23758, 36725, 14851, 17082, 5172, 2288, 21984, 32239, 4032, 23275, 2304, 8608, 49628, 7649, 4117, 13672, 16095, 46599, 35571, 3215, 4117, 36725, 8592, 3224, 3215, 14739, 20193, 11933, 43761, 11933, 15040, 21984, 36725, 8592, 3224, 3215, 14739, 3714, 35571, 3215, 14111, 995, 6225, 44510, 3224, 4032, 2288, 3794, 12610, 3224], "avg_logprob": -0.09857537100712459, "compression_ratio": 1.9791666666666667, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 1217.56, "end": 1218.48, "word": "نكتفي", "probability": 0.9156494140625}, {"start": 1218.48, "end": 1218.86, "word": " في", "probability": 0.91455078125}, {"start": 1218.86, "end": 1219.36, "word": " هذه", "probability": 0.96728515625}, {"start": 1219.36, "end": 1220.34, "word": " المحاضرة", "probability": 0.9544677734375}, {"start": 1220.34, "end": 1220.78, "word": " بهذا", "probability": 0.921630859375}, {"start": 1220.78, "end": 1221.16, "word": " القدر", "probability": 0.99462890625}, {"start": 1221.16, "end": 1221.38, "word": " على", "probability": 0.81787109375}, {"start": 1221.38, "end": 1221.58, "word": " أن", "probability": 0.66259765625}, {"start": 1221.58, "end": 1222.04, "word": " نستكمل", "probability": 0.879150390625}, {"start": 1222.04, "end": 1222.18, "word": " ان", "probability": 0.51611328125}, {"start": 1222.18, "end": 1222.32, "word": " شاء", "probability": 0.97607421875}, {"start": 1222.32, "end": 1222.64, "word": " الله", "probability": 0.9306640625}, {"start": 1222.64, "end": 1223.44, "word": " في", "probability": 0.93701171875}, {"start": 1223.44, "end": 1225.24, "word": " المحاضرات", "probability": 0.98779296875}, {"start": 1225.24, "end": 1226.04, "word": " القادمة", "probability": 0.99560546875}, {"start": 1226.04, "end": 1226.78, "word": " باقي", "probability": 0.8806966145833334}, {"start": 1226.78, "end": 1227.7, "word": " العوارض", "probability": 0.954833984375}, {"start": 1227.7, "end": 1229.12, "word": " المكتسبة", "probability": 0.91474609375}, {"start": 1229.12, "end": 1229.58, "word": " أقول", "probability": 0.830322265625}, {"start": 1229.58, "end": 1230.0, "word": " قولي", "probability": 0.94921875}, {"start": 1230.0, "end": 1230.3, "word": " هذا", "probability": 0.9501953125}, {"start": 1230.3, "end": 1231.46, "word": " وأستغفر", "probability": 0.88251953125}, {"start": 1231.46, "end": 1231.62, "word": " الله", "probability": 0.92529296875}, {"start": 1231.62, "end": 1231.82, "word": " لي", "probability": 0.8857421875}, {"start": 1231.82, "end": 1232.32, "word": " ولكم", "probability": 0.9689127604166666}, {"start": 1232.32, "end": 1232.86, "word": " سبحانك", "probability": 0.84814453125}, {"start": 1232.86, "end": 1233.32, "word": " اللهم", "probability": 0.922607421875}, {"start": 1233.32, "end": 1234.56, "word": " وبحمدك", "probability": 0.8892822265625}, {"start": 1234.56, "end": 1234.98, "word": " وأشهد", "probability": 0.9256591796875}, {"start": 1234.98, "end": 1235.16, "word": " أن", "probability": 0.86865234375}, {"start": 1235.16, "end": 1235.32, "word": " لا", "probability": 0.95751953125}, {"start": 1235.32, "end": 1235.6, "word": " إله", "probability": 0.954833984375}, {"start": 1235.6, "end": 1235.88, "word": " إلا", "probability": 0.931396484375}, {"start": 1235.88, "end": 1236.12, "word": " الله", "probability": 0.92529296875}, {"start": 1236.12, "end": 1236.6, "word": " وأشهد", "probability": 0.8917236328125}, {"start": 1236.6, "end": 1236.86, "word": " أن", "probability": 0.951171875}, {"start": 1236.86, "end": 1237.98, "word": " محمدًا", "probability": 0.83779296875}, {"start": 1237.98, "end": 1238.52, "word": " عبده", "probability": 0.9873046875}, {"start": 1238.52, "end": 1239.24, "word": " ورسوله", "probability": 0.9796875}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 1255.549375, "duration_after_vad": 1213.6618749999996}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/jDlDaN-9ALs_postprocess.srt b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/jDlDaN-9ALs_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..6d1986f16d3e18d827f049df41a69270d888c5de
--- /dev/null
+++ b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/jDlDaN-9ALs_postprocess.srt
@@ -0,0 +1,1316 @@
+1
+00:00:05,200 --> 00:00:07,800
+أعوذ بالله من الشيطان الرجيم بسم الله الرحمن
+
+2
+00:00:07,800 --> 00:00:12,640
+الرحيم إن الحمد لله نحمده و نستعينه و نستغفره و
+
+3
+00:00:12,640 --> 00:00:16,820
+نعوذ بالله من شرور أنفسنا و سيئات أعمالنا ما يهده
+
+4
+00:00:16,820 --> 00:00:20,900
+الله فلا مضل له و ما يضلل فلا هادي له و أشهد أن لا
+
+5
+00:00:20,900 --> 00:00:25,300
+إله إلا الله و أشهد أن محمدا عبده و رسوله و بعد
+
+6
+00:00:25,300 --> 00:00:30,050
+بداية أخوات الحبيبات و بنات الطيباتأحييكم بتحية
+
+7
+00:00:30,050 --> 00:00:34,350
+طيبة مباركة من عند الله السلام عليكم ورحمة الله
+
+8
+00:00:34,350 --> 00:00:38,490
+وبركاته اللهم أعلمنا ماينفعنا وانفعنا بما أعلمتنا
+
+9
+00:00:38,490 --> 00:00:44,990
+وازدنا علما اللهم آمين ننطلق الآن إلى رحلة الأحكام
+
+10
+00:00:44,990 --> 00:00:52,330
+الشرعية هيكون حديثنا فيها عن الحاكم والحكم الشرعي
+
+11
+00:00:52,330 --> 00:00:58,460
+والمحكوم فيه وأخيرا المكلف وهو المحكوم عليهأما
+
+12
+00:00:58,460 --> 00:01:06,740
+الحاكم فهو بمعنى منشئ الحكم أي الذي يصدر عنه الحكم
+
+13
+00:01:06,740 --> 00:01:13,060
+وهو الله سبحانه وتعالى فلا حاكم سوى الله ولا حكم
+
+14
+00:01:13,060 --> 00:01:20,320
+إلا ما حكم به ولا شرع إلا من الله تعالى طبعا كل من
+
+15
+00:01:20,320 --> 00:01:28,490
+يخالف ويتبع تشريع غير الله تعالىفهو كافر بإجماع
+
+16
+00:01:28,490 --> 00:01:35,930
+المسلمين و أدل على ذلك كثيرا كقوله عز وجل إن الحكم
+
+17
+00:01:35,930 --> 00:01:42,450
+إلا لله أمر الله تعبده إلا إيا الآن سنتحدث عن
+
+18
+00:01:42,450 --> 00:01:47,470
+الحكم الشرعي وأقسامه تبقى قعد معايا كويس لأن هذا
+
+19
+00:01:47,470 --> 00:01:55,880
+موضوع مهم جدا أما الحكم معناه في اللغةيطلق على
+
+20
+00:01:55,880 --> 00:02:01,940
+القضاء والحكمة والحكم يأتي بمعنى المانعة أما في
+
+21
+00:02:01,940 --> 00:02:07,300
+الإصطلاح فهو خطاب الله تعالى المتعلق بأفعال
+
+22
+00:02:07,300 --> 00:02:13,660
+المكلفين اقتضاء أو تخيير أو وضع نأتي الآن لأقسام
+
+23
+00:02:13,660 --> 00:02:20,460
+الحكم الشرعي طبعا العلماء اختلفوا في بيان أقسام
+
+24
+00:02:20,460 --> 00:02:24,920
+الحكم الشرعيمنهم ما نجعله قسمًا واحدًا وهو الحكم
+
+25
+00:02:24,920 --> 00:02:29,040
+التكليفي ومنهم ما نجعله قسمين الحكم التكليفي
+
+26
+00:02:29,040 --> 00:02:33,580
+والحكم الوضعي والسبب اختلافهم في تعريفه وهذا
+
+27
+00:02:33,580 --> 00:02:37,600
+الكلام باختصار شديد والمنهج اللي إحنا هنمشي عليه
+
+28
+00:02:37,600 --> 00:02:43,120
+في تقسيم الحكم الشرعي هنتابع المنهج التاني وهو أن
+
+29
+00:02:43,120 --> 00:02:49,220
+الحكم الشرعي ينقسم إلى قسمين التكليفي والوضعي إذا
+
+30
+00:02:49,540 --> 00:02:54,640
+أقسام الحكم الشرعي اللي هنعتمدهم القسم الأول الحكم
+
+31
+00:02:54,640 --> 00:03:01,000
+التكليفي والقسم الثاني الحكم الوطني نبدأ بالحكم
+
+32
+00:03:01,000 --> 00:03:07,620
+التكليفي بالنسبالي الحكم التكليفي هو خطاب الشارع
+
+33
+00:03:07,620 --> 00:03:13,940
+المتعلق بأفعال المكلفين بالاقتضاء أو التخيير طبعا
+
+34
+00:03:13,940 --> 00:03:18,920
+كلمة الاقتضاء هنا وكلمة التخيير بتشتمل على
+
+35
+00:03:19,600 --> 00:03:25,620
+التقسيمات الحكم التكليفي تمام؟ تجتمل على تقسيمات
+
+36
+00:03:25,620 --> 00:03:29,120
+الحكم التكليفي خلينا نشوف هذه التقسيمات على السريع
+
+37
+00:03:29,120 --> 00:03:35,960
+مع بعض طبعا كلمة الاقتضاء معناها الطلب والطلب
+
+38
+00:03:35,960 --> 00:03:44,260
+ينقسم إلى قسمين طلب فعل وطلب ترك إن كان طلب الفعل
+
+39
+00:03:44,260 --> 00:03:51,760
+طلبا جازما فهو الإيجاب، هذه القسم الأولوإن كان طلب
+
+40
+00:03:51,760 --> 00:03:59,280
+الفعل طلبا غير جازم فهو الندم أما القسم الثاني طلب
+
+41
+00:03:59,280 --> 00:04:08,600
+الترك إن كان طلب الترك طلبا جازما فهو التحريم وإن
+
+42
+00:04:08,600 --> 00:04:13,300
+كان طلب الترك طلبا غير جازم فهو إيه؟ فهو الكراهة
+
+43
+00:04:13,300 --> 00:04:18,360
+لحظة معايا صار في عندي الإجاب والندب والتحريم
+
+44
+00:04:18,360 --> 00:04:23,580
+والكراهةبيظل عندي إيش؟ بيظل عندي التخيير اللي هو
+
+45
+00:04:23,580 --> 00:04:29,200
+إيه اللي باعها اللي هو يستوي الفعل والترج إذا عندي
+
+46
+00:04:29,200 --> 00:04:33,360
+الطلب كلمة اقتضاء هذه لحالها معناها إيش؟ معناها
+
+47
+00:04:33,360 --> 00:04:39,080
+طلب بيندرج فيها تحتها الأقسام الأربعة للحكم
+
+48
+00:04:39,080 --> 00:04:43,500
+التكليفي اللي هو الإيجاب زي ما أنتوا شايفين و
+
+49
+00:04:43,500 --> 00:04:47,060
+الندم و التحريم و القراها كله هاي من كلمة واحدة في
+
+50
+00:04:47,060 --> 00:04:51,670
+التعريف والتخيير اللي هو رقم خمسةاللي هي إيه؟ اللي
+
+51
+00:04:51,670 --> 00:04:58,210
+هو الإباحة، تمام؟ إذا الحكم التكليفي كمان مرة هو
+
+52
+00:04:58,210 --> 00:05:04,250
+خطاب الشارع المتعلق بأفعال المكلفين بالاقتضاء أو
+
+53
+00:05:04,250 --> 00:05:11,930
+التخيير، تمام؟ نأتي الآن إلى أقسام الحكم التكليفي
+
+54
+00:05:11,930 --> 00:05:17,330
+ومتعلقاتهطبعا المتعلق الحكم التكليفي هو من الله
+
+55
+00:05:17,330 --> 00:05:21,990
+سبحانه وتعالى والمتعلق بنا نحن المكلفين ونفعل
+
+56
+00:05:21,990 --> 00:05:27,810
+التكليف الذي طلبه الله سبحانه وتعالى مننا طبعا في
+
+57
+00:05:27,810 --> 00:05:31,110
+خلاف عند العلماء في بيان أقسام الحكم التكليفي
+
+58
+00:05:31,110 --> 00:05:37,250
+عندنا الحنافية لهم تقسيمات هنشوفها مع بعض و
+
+59
+00:05:37,250 --> 00:05:43,320
+الجمهور لهم تقسيماتحنذكر كل تقسيم من هذه التقسيمات
+
+60
+00:05:43,320 --> 00:05:49,300
+لكل فريق من الجمهور كل من الجمهور والحنفية بإذن
+
+61
+00:05:49,300 --> 00:05:54,960
+الله تعالى نبدأ بأقسام الحكم التكليفي عند الجمهور
+
+62
+00:05:54,960 --> 00:06:03,580
+وبيان متعلقاته القسم الأول وهو الإيجاب ومتعلقه
+
+63
+00:06:03,580 --> 00:06:12,920
+الواجب القسم الثاني الندبومتعلق المندوب أما القسم
+
+64
+00:06:12,920 --> 00:06:19,840
+الثالث فهو التحريم ومتعلق الحرام أو المحرم والقسم
+
+65
+00:06:19,840 --> 00:06:26,960
+الرابع الكراهة ومتعلقها المكروه أما القسم الأخير
+
+66
+00:06:26,960 --> 00:06:33,620
+عند جمهور العلماء هو الإباحة ومتعلق المباح إذن
+
+67
+00:06:33,620 --> 00:06:37,800
+الأقسام الحكم التكليفي عند الجمهور خمسةاللي قبل
+
+68
+00:06:37,800 --> 00:06:42,960
+شوية وضحناهم من خلال كلمة الاقتضاء في التعريف كل
+
+69
+00:06:42,960 --> 00:06:50,900
+قسم له متعلق الحكم الإيجاب ومتعلقه الواجب الندب
+
+70
+00:06:50,900 --> 00:06:56,040
+ومتعلق المندوب التحريم ومتعلق الحرام أو المحرم
+
+71
+00:06:56,040 --> 00:07:02,860
+الكراهة ومتعلقها المكروه الإباحة ومتعلقها المباح
+
+72
+00:07:02,860 --> 00:07:09,590
+طبعا هذه الأقسام بدك تحفظيها زي اسمكتمام؟ كل قسم و
+
+73
+00:07:09,590 --> 00:07:18,450
+المتعلق الذي يتعلق به نيجي لتعريف كل قسم من هذه
+
+74
+00:07:18,450 --> 00:07:23,410
+الأقصام و تعريف متعلقه و نجيب مثل عليه الآن هنجيب
+
+75
+00:07:23,410 --> 00:07:28,150
+كل قسم من هذه الأقصام اللي هي أقصام الحكم التكليفي
+
+76
+00:07:28,150 --> 00:07:34,530
+عند الجمهور و هنتعرف على معناه سواء هو أو المتعلق
+
+77
+00:07:34,530 --> 00:07:41,880
+الذي يتعلق به حكيناالإيجاب متعلق الواجب الإيجاب هو
+
+78
+00:07:41,880 --> 00:07:47,940
+خطاب الشرع الطالب للفعل طلبا جازما ركزي معايا
+
+79
+00:07:47,940 --> 00:07:53,460
+الإيجاب هو خطاب من مين؟ من المشرع الطالب للفعل
+
+80
+00:07:53,460 --> 00:07:58,600
+طلبا جازما هذا هو الحكم طيب إيش المتعلق تبع الحكم
+
+81
+00:07:58,600 --> 00:08:02,760
+اللي هو التنفيذ تبعنا اللي بتعلق فينا إحنا كمكلفين
+
+82
+00:08:02,760 --> 00:08:09,880
+متعلق الإيجاب الواجبالواجب هو ما طلب الشرع فعله
+
+83
+00:08:09,880 --> 00:08:16,880
+طلبا جاذما نجيب مثال عليه قول الله تعالى و أقيموا
+
+84
+00:08:16,880 --> 00:08:21,320
+الصلاة و آتوا الذكاء و أقيموا الصلاة و آتوا الذكاء
+
+85
+00:08:21,320 --> 00:08:26,580
+تمام؟ إذا الان مطلوب مني كل قسم من هذه الأقسام
+
+86
+00:08:26,580 --> 00:08:31,420
+تعريفه و تعريف متعلقه و نجيب مثال عليه تمام؟ و هذا
+
+87
+00:08:31,420 --> 00:08:35,660
+الكلام بدك تبدأ تحفظ الآن التعريفاتخد بالك وانت
+
+88
+00:08:35,660 --> 00:08:40,340
+بتحفظ التعريف، بنفعش تنقص كلمة من التعريف وبنفعش
+
+89
+00:08:40,340 --> 00:08:44,420
+تزودي كلمة على التعريف، لازم التعريف زي ما هو، هكي
+
+90
+00:08:44,420 --> 00:08:49,860
+تمام؟ هذا استضاحه، هذا تعريف، لازم نحفظه زي ما هو،
+
+91
+00:08:49,860 --> 00:08:53,840
+يعني لو روحت كتب عليه التعريف ناقص، رح أحط عليه
+
+92
+00:08:53,840 --> 00:08:58,600
+غلط، مش رح أحط عليه نص العلامة أو كتبت التعريف فيه
+
+93
+00:08:58,600 --> 00:09:02,760
+زيادة أو ألفتيه من عندك، بنفعش، لازم التعريف زي ما
+
+94
+00:09:02,760 --> 00:09:10,990
+هو موجودخلاص اتفقنا؟ تمام متفقين نكمل الآن الندب
+
+95
+00:09:10,990 --> 00:09:17,670
+ومتعلقه المندوب الندب هو خطاب الشارع الطالب للفعل
+
+96
+00:09:17,670 --> 00:09:25,170
+طلبا غير جازم والمندوب هو ما طلب الشارع فعله طلبا
+
+97
+00:09:25,170 --> 00:09:30,800
+غير جازمومثال عليه قول الله تعالى يا أيها الذين
+
+98
+00:09:30,800 --> 00:09:36,580
+آمنوا إذا دا داينتم بدين إلى أجل مسمن فاكتبوه أما
+
+99
+00:09:36,580 --> 00:09:42,200
+القسم الثالث فهو التحريم ومتعلق الحرام أو المحرم
+
+100
+00:09:42,200 --> 00:09:49,040
+أما التحريم هو خطاب الشرع المقتضي ترك الفعل اقتضاء
+
+101
+00:09:49,040 --> 00:09:56,220
+جازما أما المحرم فهو ما طلب الشرع تركه طلبا جازما
+
+102
+00:09:56,990 --> 00:10:02,070
+ومثال عليه قول الله تعالى ولا تقتل النفس التي حرم
+
+103
+00:10:02,070 --> 00:10:07,970
+الله إلا بالحق أما القسم الرابع فهو الكراهة
+
+104
+00:10:07,970 --> 00:10:14,170
+ومتعلقه المكروه أما الكراهة فهي خطاب الشرع المقتضي
+
+105
+00:10:14,170 --> 00:10:19,930
+ترك الفعل اقتضاء غير جازم أما المكروه هو ما طلب
+
+106
+00:10:19,930 --> 00:10:26,910
+الشرع تركه طلبا غير جازم ومثال عليهقول النبي صلى
+
+107
+00:10:26,910 --> 00:10:32,550
+الله عليه وسلم أكل من أكل البصلة أو الثوم فليعتزل
+
+108
+00:10:32,550 --> 00:10:38,310
+مجلسنا هذا في قول النبي صلى الله عليه وسلم إذا دخل
+
+109
+00:10:38,310 --> 00:10:47,550
+أحدكم المسجد فلا يجلسان حتى يصلي ركعتين أخر
+
+110
+00:10:47,550 --> 00:10:54,500
+قسم من هذه الأقسام الإباحة ومتعلقها المباحالإباحة
+
+111
+00:10:54,500 --> 00:10:58,540
+هي خطاب الشرع المتعلق بأفعال المكلفين على وجه
+
+112
+00:10:58,540 --> 00:11:04,260
+التخيير بين الفعل والترك أما المباح هو ما خيّر
+
+113
+00:11:04,260 --> 00:11:09,440
+الشارع فيه بين الفعل والترك من غير اقتضاء ولا زجر
+
+114
+00:11:09,440 --> 00:11:16,520
+مثال عليه قول الله تعالى فانكحوا ما طاب لكم باق
+
+115
+00:11:16,520 --> 00:11:22,070
+الآية كلوا مما في الأرض حلالا طيبةتمام؟ هذا الحكم
+
+116
+00:11:22,070 --> 00:11:30,910
+المباح نقتل الآن لتقسيم الحكم الشرعي عند الحنافية
+
+117
+00:11:30,910 --> 00:11:34,930
+ومتعلقاته احنا حكينا عند الجمهور عندنا خمس أقسام
+
+118
+00:11:34,930 --> 00:11:40,190
+طيب عند الحنافية تعالوا نشوف مع بعض أكام قسم القسم
+
+119
+00:11:40,190 --> 00:11:49,490
+الأول هو الافتراض ومتعلقه الفرض قسم ثاني الإيجاب
+
+120
+00:11:50,200 --> 00:11:55,540
+ومتعلقه الواجب لحظة احنا في صار فيه ازيادة في
+
+121
+00:11:55,540 --> 00:11:59,880
+الأقسام وهذه ازيادة لها سبب الآن هنذكره بإذن الله
+
+122
+00:11:59,880 --> 00:12:06,200
+تعالى أما القسم الثالث فهو الندب ومتعلقه المندوب
+
+123
+00:12:06,200 --> 00:12:13,360
+التحريم ومتعلقه الحرام أو المحرم الكراها التحريمية
+
+124
+00:12:13,360 --> 00:12:19,490
+ومتعلقها المكروه تحريما الكراها التنزيهيةومتعلقها
+
+125
+00:12:19,490 --> 00:12:25,570
+المكروه تنزيها الإباحة ومتعلقها المباحة يللا نعدهم
+
+126
+00:12:25,570 --> 00:12:33,170
+مع بعض الأقسام عندنا الافتراض الإيجاب 2 الندب 3
+
+127
+00:12:33,170 --> 00:12:38,190
+تحريم 4 كراها التحريمية 5 كراها التنزيهية 6
+
+128
+00:12:38,190 --> 00:12:46,430
+الإباحة 7 يعني الأحناف زادوا قسمين على الأقسامعلى
+
+129
+00:12:46,430 --> 00:12:51,290
+أقسام الحكم التكليفي عند الجمهور طبعا هو الافتراض
+
+130
+00:12:51,290 --> 00:12:57,230
+هذا الزيادة والكرهة القسموها
+
+131
+00:12:57,230 --> 00:13:02,390
+إلى قسمين الكراهة تحريمية وكرهة تنزيهية تعالى نشوف
+
+132
+00:13:02,390 --> 00:13:06,310
+تعريف كل قسم من هذه الأقسام وحكينا التعريفات لازم
+
+133
+00:13:06,310 --> 00:13:11,730
+نحفظها زي ما هي لا بدنا نزود عليها ولا بدنا نقص
+
+134
+00:13:11,730 --> 00:13:16,200
+عليها تعالى نشوف مع بعضتعريف الإفتراض وتعريف
+
+135
+00:13:16,200 --> 00:13:21,820
+متعلقه الفرض أما الإفتراض هو خطاب الشارع الطالب
+
+136
+00:13:21,820 --> 00:13:27,240
+للفعل طلبا جازما بدليل قطعي خلوا بالكم معايا في
+
+137
+00:13:27,240 --> 00:13:34,200
+التعريف كمان مرة الإفتراض هو خطاب الشارع الطالب
+
+138
+00:13:34,200 --> 00:13:42,180
+للفعل طلبا جازما بدليل قطعي أما الفرضوهو ما ثبت
+
+139
+00:13:42,180 --> 00:13:48,060
+بدليل قطعي واستحق الذنب على تركه مطلقا من غير عذره
+
+140
+00:13:48,060 --> 00:13:53,400
+أما مثال على ما نقول قول الله تعالى وأقيموا الصلاة
+
+141
+00:13:53,400 --> 00:13:59,620
+وآتوا الزكاة نأتي للإيجاب وقبل شوية عرفناه مش
+
+142
+00:13:59,620 --> 00:14:05,740
+هنجيب حاجة جديدة الآن الإيجاب ومتعلقه الواجب لكن
+
+143
+00:14:05,740 --> 00:14:10,730
+الأحناف هيزيدوا قيد في التعريف علىتعريف الجمهور
+
+144
+00:14:10,730 --> 00:14:17,010
+هنشوفه إيه الآن الإجاب هو خطاب الشارع الطالب للفعل
+
+145
+00:14:17,010 --> 00:14:22,710
+طلبا جاذما بدليل ظني اخدين بالكوا هناك الإفتراض
+
+146
+00:14:22,710 --> 00:14:29,250
+الطلب الجاذم ثابت بإيش؟ بدليل قطعي هنا ثابت بإيش؟
+
+147
+00:14:29,250 --> 00:14:36,130
+بدليل ظني اللي هو الإجاب أما الواجب ما ثبت بدليل
+
+148
+00:14:36,130 --> 00:14:43,320
+ظنيواستحق الذنب على تركه مطلقا بغير عذر مثال عليه
+
+149
+00:14:43,320 --> 00:14:48,160
+قول النبي صلى الله عليه وسلم لصلاة لمن لم يقرأ
+
+150
+00:14:48,160 --> 00:14:57,140
+بفاتحات الكتاب الندب ومتعلق المندوب عند الأحناف
+
+151
+00:14:57,140 --> 00:15:03,280
+الندب هو خطاب الشارع الطالب للفعل طلبا غير جازم
+
+152
+00:15:03,280 --> 00:15:09,570
+أما المندوبالمطلوب فعله شرعا من غير ذمن على تركه
+
+153
+00:15:09,570 --> 00:15:15,110
+مطلقا ومثال عليه قول الله عز وجل يا أيها الذين
+
+154
+00:15:15,110 --> 00:15:21,090
+آمنوا إذا تدينتم بدين إلى أجل مسمن فاكتبوه أما
+
+155
+00:15:21,090 --> 00:15:27,730
+القسم الرابع التحريم ومتعلقه الحرام أو المحرم
+
+156
+00:15:27,730 --> 00:15:33,670
+التحريم هو خطاب الشارع الطالب ترك الفعل طلبا جازما
+
+157
+00:15:33,670 --> 00:15:40,900
+بدليل قطعيأما المحرم تعريفه وهو متعلق الحكم
+
+158
+00:15:40,900 --> 00:15:47,240
+التكليفي اللي هو التحريم ما ثبت تركه بدليل قطعي
+
+159
+00:15:47,240 --> 00:15:53,820
+واستحق الذنب على فعله من غير عذر ومثال عليه قول
+
+160
+00:15:53,820 --> 00:15:59,900
+الله عز وجل ولا تقتل النفس التي حرم الله إلا بالحق
+
+161
+00:15:59,900 --> 00:16:06,480
+أما الكراهة التحريميةومتعلقها المكروه تحريما
+
+162
+00:16:06,480 --> 00:16:12,660
+الكراها التحريمية هي خطاب الشارع الطالب ترك الفعل
+
+163
+00:16:12,660 --> 00:16:17,620
+طلبا جازما بدليلا ظنين في حد بيسأل عندها ويقول له
+
+164
+00:16:17,620 --> 00:16:21,260
+هذا كله بدنا نحفظه؟ أه كله بدنا نحفظه احنا في
+
+165
+00:16:21,260 --> 00:16:25,180
+الأول لما حفظنا أقصام الحكم التكليفي عند الجمهور
+
+166
+00:16:25,180 --> 00:16:30,720
+كانوا خمسة وانا عند الحنافية صاروا سبعة يعني زودنا
+
+167
+00:16:30,720 --> 00:16:35,010
+اتنينيعني ماجيبناش حاجة جديدة تقريبا إلا القسمين
+
+168
+00:16:35,010 --> 00:16:37,810
+الاجداد هدول يعني معناته لما تحفظ الخمسة
+
+169
+00:16:37,810 --> 00:16:41,550
+الأولانيين بيضل عليك اتنين على الخمسة هدول مع
+
+170
+00:16:41,550 --> 00:16:49,650
+فوارق التعريف لما تحفظيه هيسهل عليك أنك أول حاجة
+
+171
+00:16:49,650 --> 00:16:52,550
+بتلخصيه عشان يسهل عليك إن شاء الله حفظه وما
+
+172
+00:16:52,550 --> 00:16:56,770
+تتغلبيش فيه هتعدي مرة مرتين إلا أنت حافظها إذا
+
+173
+00:16:56,770 --> 00:17:01,470
+نرجع نكمل تاني الكراهة التحريمية ومتعلقهاالمكروه
+
+174
+00:17:01,470 --> 00:17:06,790
+تحريما والكراهة التحريمية هي خطاب الشارع الطالب
+
+175
+00:17:06,790 --> 00:17:12,530
+ترك الفعل طلبا جازما بدليل ظنى أما المكروه تحريما
+
+176
+00:17:12,530 --> 00:17:18,590
+وهو متعلق الكراهة التحريمية هي أو هو ما طلب الشارع
+
+177
+00:17:18,590 --> 00:17:25,050
+من المكلف الكف عنه حتما بدليل ظنى لقطعى طبعا مثال
+
+178
+00:17:25,050 --> 00:17:28,580
+عليهقول النبي صلى الله عليه وسلم لا يبيع أحدكم على
+
+179
+00:17:28,580 --> 00:17:36,420
+بيع أخيه ولا يخطب على خطبة أخيه إلا أن يأذن له أما
+
+180
+00:17:36,420 --> 00:17:44,220
+الكراها التنزيهية لمتعلقها المكروه تنزيها والكراهة
+
+181
+00:17:44,220 --> 00:17:50,860
+التنزيهية هي خطاب الشارع الطالب ترك الفعل طلبا غير
+
+182
+00:17:50,860 --> 00:17:58,350
+جازم أما المكروه تنزيهاهو ما طلب الشارع تركه لا
+
+183
+00:17:58,350 --> 00:18:04,850
+على وجه الحتمي والالزام ومثال عليه زي ما حكينا قبل
+
+184
+00:18:04,850 --> 00:18:09,690
+قليل قول النبي صلى الله عليه وسلم من أكل ثوما أو
+
+185
+00:18:09,690 --> 00:18:18,170
+بصلا فلا يقربن مصلانا أما الحكم الأخير فهو الإباحة
+
+186
+00:18:18,170 --> 00:18:23,290
+ومتعلق المباح والإباحة هي تخيير الشارع بين الفعل
+
+187
+00:18:23,290 --> 00:18:28,140
+والتركولا مدح ولا ذم على الفعل والترك ويقال لها
+
+188
+00:18:28,140 --> 00:18:32,720
+الحلال يعني بيطلقوا عليها بعض العلماء اسم الحلال
+
+189
+00:18:32,720 --> 00:18:37,520
+أو لفظ الحلال أو مصطلح الحلال المباح ما خيّر
+
+190
+00:18:37,520 --> 00:18:43,800
+الشارع فيه بين فعله وتركه ومثال عليه قول الله عز
+
+191
+00:18:43,800 --> 00:18:47,400
+وجل فانكحوا ما طاب لكم إلى أخر الأية كلوا مما في
+
+192
+00:18:47,400 --> 00:18:52,860
+الأرض حلالا طيبة لاحظوا ان بعد استعراض هذه الأقسام
+
+193
+00:18:53,190 --> 00:18:57,730
+حاولت أنا أن الأمثلة نفس الأمثلة اللي طرحتها في
+
+194
+00:18:57,730 --> 00:19:02,130
+أقسام الحكم التكليفي عند جمهور العلماء عشان ما
+
+195
+00:19:02,130 --> 00:19:06,550
+نتلخبطش وما نجيبش حاجة جديدة يعني الآن مطلوب مني
+
+196
+00:19:06,550 --> 00:19:11,310
+أقسام الحكم التكليفي عند الجمهور الخامسة كل قسم
+
+197
+00:19:11,310 --> 00:19:15,210
+ومتعلقه تعريف كل قسم وتعريف المتعلق مثال عليه
+
+198
+00:19:15,210 --> 00:19:19,650
+ننتقل إلى الحكم التكليفي عند الحنافية برضه هنجيب
+
+199
+00:19:19,650 --> 00:19:24,270
+تعريف كل قسم ومتعلقهطبعا متعلق زي ما حكينا بقين
+
+200
+00:19:24,270 --> 00:19:29,250
+بفعل المكلف أو بالمكلف ربنا سبحانه وتعالى يكلفنا
+
+201
+00:19:29,250 --> 00:19:35,110
+بالحكم هي الحكم الإباحة والمتعلق اللي هو فينا في
+
+202
+00:19:35,110 --> 00:19:40,730
+فعلنا اللي هو المباحي تمام؟ إذا كمان مرة بنأكد على
+
+203
+00:19:40,730 --> 00:19:49,680
+أنه كل الأقسام هذه مطلوبة تحفظ تعريفاتها بدقةيعني
+
+204
+00:19:49,680 --> 00:19:53,740
+مابنفعش نسيب ولا كلمة و مابنفعش نزيد ولا كلمة و
+
+205
+00:19:53,740 --> 00:19:57,800
+مابنفعش نقلف تعريف من عندنا بالمعنى يعني بدي أجيب
+
+206
+00:19:57,800 --> 00:20:01,840
+المعنى كما هو عشان تسهلي على حالك بتروحي عاملة
+
+207
+00:20:01,840 --> 00:20:07,840
+جدول بطريقة جميلة هيك تكون واضحة و بتروحي كاتبة في
+
+208
+00:20:07,840 --> 00:20:12,580
+الأقسام هذه و متعلقات الأقسام هذه و بتروحي حافظاها
+
+209
+00:20:12,580 --> 00:20:16,760
+مفيش بتقريها مرة مرتين إلا أنت ان شاء الله بتكون
+
+210
+00:20:16,760 --> 00:20:23,070
+محفوظةنقطة مش مفهومة ان شاء الله في اللقاء المفتوح
+
+211
+00:20:23,070 --> 00:20:28,850
+بيننا حضروا الأسئلة عشان نجاوب عليها و نفصل فيها
+
+212
+00:20:28,850 --> 00:20:35,690
+بإذن الله تعالى و نوضحها نأتي الآن للفرق بين الفرض
+
+213
+00:20:35,690 --> 00:20:40,510
+و الواجب تكلمنا قبل قليل في أقسام الحكم التكليفي
+
+214
+00:20:40,510 --> 00:20:46,170
+عند الجمهور و عند الأحناف وجدنا أن الأحناف قالوا
+
+215
+00:20:46,170 --> 00:20:51,340
+في عناحاجة اسمها فرض وفي عندنا حاجة اسمها واجب أما
+
+216
+00:20:51,340 --> 00:20:54,760
+الجمهور قالوا لأ احنا عندنا الفرض و الواجب واحد
+
+217
+00:20:54,760 --> 00:21:00,200
+طيب السؤال اللي بيترح نفسه الآن ما الفرق بين الفرض
+
+218
+00:21:00,200 --> 00:21:07,560
+و الواجب الفعل عند الجمهور يسمى واجبا كما يسمى
+
+219
+00:21:07,560 --> 00:21:14,140
+فرضا ليش؟ هي الجواب لأنهم لم ينظروا إلى الدليل من
+
+220
+00:21:14,140 --> 00:21:20,070
+جهة قطعيته أو ظنيتهإنما نظروا إلى طبيعة الفعل قالك
+
+221
+00:21:20,070 --> 00:21:25,370
+إذا طبيعة الفعل إلزامي فهو واجب وإن كان غير إلزامي
+
+222
+00:21:25,370 --> 00:21:31,110
+فهو المندوب سواء كان ثابت بدليل قطعي أم دليل ظني،
+
+223
+00:21:31,110 --> 00:21:37,470
+هيك واضحط الأمور، إذا جمهور العلماء قالوا الفرض
+
+224
+00:21:37,470 --> 00:21:42,790
+والواجب عندنا واحد زي ما بنسمي الواجب بنسميه واجب
+
+225
+00:21:42,790 --> 00:21:48,930
+كمان بنسميه فرض مافيش فرق بينهمليش؟ لأنهم لم
+
+226
+00:21:48,930 --> 00:21:53,950
+ينظروا إلى الدليل من جهة قطعيته أو ظنيته، لكن
+
+227
+00:21:53,950 --> 00:21:57,330
+نظروا إلى الدليل من ناحية إيه؟ من ناحية طبيعة
+
+228
+00:21:57,330 --> 00:22:02,010
+الفعل طبيعة الفعل، إذا كان فيه جهة إلزامية، قالك
+
+229
+00:22:02,010 --> 00:22:06,090
+هذا واجب، إن كان مش إلزامية، قالك هذا مندوب سواء
+
+230
+00:22:06,090 --> 00:22:12,630
+كان ثابت بدليل قطعي أو دليل ظني، تمام؟ طيب، تعالوا
+
+231
+00:22:12,630 --> 00:22:18,050
+نشوف الأحناف، طيبلماذا يفرقون بين الفرض والواجب
+
+232
+00:22:18,050 --> 00:22:24,310
+بقول الفرض عندهم ما ثبت بدليل قطعي انتهينا ما هو
+
+233
+00:22:24,310 --> 00:22:28,870
+في التعريف اللي قبل شوية حكينا الافتراض ومتعلقها
+
+234
+00:22:28,870 --> 00:22:34,850
+الفرض وهو ثابت بدليل قطعي أما الواجب ما ثبت بدليل
+
+235
+00:22:34,850 --> 00:22:41,690
+ظنني تمام؟ إذا جمهور العلماء نظروا إلى طبيعة الفعل
+
+236
+00:22:41,690 --> 00:22:50,130
+أما الأحناف نظروا إلىثبوت الحكم بالدليل لو كان
+
+237
+00:22:50,130 --> 00:22:55,090
+الدليل قطعي بيكون فرض لو كان الدليل ظنى بيكون ايه؟
+
+238
+00:22:55,090 --> 00:23:02,190
+بيكون واجب بعض العلماء قال استنى شوية كالقامدي و
+
+239
+00:23:02,190 --> 00:23:06,630
+الرازي قال الخلاف هذا اللي بين الجمهور و الحنافية
+
+240
+00:23:06,630 --> 00:23:13,770
+خلاف لفظي يعني كأنه مافيش خلاف تمام؟ لكن لو نظرنا
+
+241
+00:23:13,770 --> 00:23:19,030
+إلىالواقع من حيث الآثار الفقهية والأحكام الشرعية
+
+242
+00:23:19,030 --> 00:23:24,890
+لو وجدنا أن الحنافية رتبوا على الخلاف بعض الآثار
+
+243
+00:23:24,890 --> 00:23:31,210
+الفقهية من ناحية الحكم كالحكم العلمي والعقائدي ومن
+
+244
+00:23:31,210 --> 00:23:35,770
+ناحية الأحكام الشرعية للفروع الفقهية من ناحية
+
+245
+00:23:35,770 --> 00:23:41,450
+الحكم اللي يكفر الإنسان بإنكار الفرض أما الواجب
+
+246
+00:23:41,450 --> 00:23:49,690
+فلا يكفر ممكنهتمام؟ من ناحية الآثار الفقهية قالوا
+
+247
+00:23:49,690 --> 00:23:56,970
+إن ترك مطلق القراءة في الصلاة يبطلها لأن الأمر بها
+
+248
+00:23:56,970 --> 00:24:02,290
+قرآني في قوله تعالى فاقرؤوا ما تيسر من القرآن هنا
+
+249
+00:24:02,290 --> 00:24:07,470
+هذا دليل عندهم قطعي والدليل القطعي بيقول اقرأوا ما
+
+250
+00:24:07,470 --> 00:24:14,730
+تيسر من القرآن في الصلاة وحكى مش شرط يكونالفاتحة
+
+251
+00:24:14,730 --> 00:24:20,250
+لكن أي شيء قرأته في الصلاة من القرآن بالدليل
+
+252
+00:24:20,250 --> 00:24:24,190
+الثابت هنا عندنا الدليل القاطع عندنا فقرؤوا ما
+
+253
+00:24:24,190 --> 00:24:28,790
+تيسر منهم فتكون صلاتك صحيحة متى بتكون صلاتك باطلة
+
+254
+00:24:28,790 --> 00:24:32,270
+إذا تركت مطلق القراءة يعني إذا ماقرأتش ولا حاجة من
+
+255
+00:24:32,270 --> 00:24:38,930
+القرآن أما ترك الفاتحة عندهم بذاتها في الصلاة فلا
+
+256
+00:24:38,930 --> 00:24:45,610
+يبطلها لأن الأمر بها ثبت بخبر الأحادأي بقوله صلى
+
+257
+00:24:45,610 --> 00:24:49,030
+الله عليه وسلم لا صلاة لمن لم يقرأ بفاتحة الكتاب
+
+258
+00:24:49,030 --> 00:24:54,270
+وهو يفيد الظن تمام؟ إذا الأثر الفقهية اللي رتبوها
+
+259
+00:24:54,270 --> 00:24:58,290
+على الخلاف العلماء بيقولوا القامدي و الرازي
+
+260
+00:24:58,290 --> 00:25:02,310
+بيقولوا الخلاف فده في تقصيق في الفرق بين الفرض
+
+261
+00:25:02,310 --> 00:25:06,590
+والواجب خلاف لفظي يعني كأنه مافيش خلاف لكن لو
+
+262
+00:25:06,590 --> 00:25:10,170
+نظرنا إلى الواقع لو وجدنا خلاف ناحية الحكم يكفر
+
+263
+00:25:10,170 --> 00:25:14,950
+الإنسان بإنكار الفرض عند الحنفيةأما بإنكار الواجب
+
+264
+00:25:14,950 --> 00:25:20,650
+فلا يكفر منكره من ناحية الأثار الفقهية وجدنا أن من
+
+265
+00:25:20,650 --> 00:25:27,590
+ترك مطلق القراءة في الصلاة يبطلها ليش؟ لأن الأمر
+
+266
+00:25:27,590 --> 00:25:32,690
+بيها قرآني ثبت بدليل قطعي فقرؤوا ما تيسر من القرآن
+
+267
+00:25:32,690 --> 00:25:37,390
+أما ترك قراءة الفاتحة بذاتها في الصلاة عندهم عند
+
+268
+00:25:37,390 --> 00:25:42,920
+الأحناف فلا يبطلهالأن الأمر بها ثبت بخبر الأحد أي
+
+269
+00:25:42,920 --> 00:25:47,060
+بقوله صلى الله عليه وسلم لصلة لمن لم يقرأ بفاتحة
+
+270
+00:25:47,060 --> 00:25:55,500
+الكتاب وهو يفيد الظن الراجح عندنا كما رجح مؤلف
+
+271
+00:25:55,500 --> 00:26:00,820
+الكتاب رجح ما ذهب إليه الجمهور واحنا من ضمن صوتنا
+
+272
+00:26:00,820 --> 00:26:05,960
+لصوته طب يقول ليش؟ السبب يعني لماذا يعني رجحت
+
+273
+00:26:05,960 --> 00:26:10,920
+الجمهور على قول الأحناف تعالوا نشوفالأسباب اللي
+
+274
+00:26:10,920 --> 00:26:17,380
+ذُكرته هذه كلها مهمة جدا أول سبب اختلاف طرق .. طرق
+
+275
+00:26:17,380 --> 00:26:20,380
+.. طرق إثبات الواجبات في القوة والضعف لا يوجب
+
+276
+00:26:20,380 --> 00:26:25,240
+اختلاف الواجب في حقيقته من حيث هو واجب يعني الواجب
+
+277
+00:26:25,240 --> 00:26:30,020
+بيظل واجب سواء ثبت بدليل قطعي أو ثبت بدليل ..
+
+278
+00:26:30,020 --> 00:26:35,240
+بدليل ايه؟ بدليل ظني كله اسمه واجب في النهاية أي
+
+279
+00:26:35,240 --> 00:26:42,250
+واحد اتنين المشرعأطلق اسم الفرض على الواجب يعني
+
+280
+00:26:42,250 --> 00:26:45,370
+ربنا سبحانه وتعالى أطلق اسم الفرض على الواجب
+
+281
+00:26:45,370 --> 00:26:50,070
+فبيكون الفرض والواجب واحد بقوله تعالى فمن فرض فيهن
+
+282
+00:26:50,070 --> 00:26:56,310
+الحج أي أوجب الحج كمان في عندي سبب أجمع العلماء
+
+283
+00:26:56,310 --> 00:26:59,410
+على إطلاق اسم الفرض على ما أدى من الصلاوات
+
+284
+00:26:59,410 --> 00:27:04,230
+المختلفة في صحتها بين الأامة ومن ذلك من صلى بغير
+
+285
+00:27:04,230 --> 00:27:09,850
+الفاتحة أدى فرض الله تعالىوالأصل فيه الإطلاق
+
+286
+00:27:09,850 --> 00:27:15,510
+الحقيقة السبب الرابع أن هذه التفريقة غير مقبولة
+
+287
+00:27:15,510 --> 00:27:21,170
+مردودة يعني التفريق بين الفرض والواجب مردود ليش؟
+
+288
+00:27:21,170 --> 00:27:26,050
+لأنه بترتب عليها أن يكون للفعل الواحد حكمان
+
+289
+00:27:26,050 --> 00:27:30,610
+مختلفان واحد بالنسبة إلنا والآخر بالنسبة للصحابي
+
+290
+00:27:30,610 --> 00:27:34,550
+اللي روى الحديث عن النبي صلى الله عليه وسلمفهو
+
+291
+00:27:34,550 --> 00:27:38,110
+بالنسبة له فرض لانتفاء الشبهة في صحة الدليل في
+
+292
+00:27:38,110 --> 00:27:44,710
+حقها وهذا إيه؟ وهذا تفريق غير مقبول لكن يعني ..
+
+293
+00:27:44,710 --> 00:27:49,350
+يعني بعد .. ولو أننا رجحنا قول الجمهور لكن بالآخر
+
+294
+00:27:49,350 --> 00:27:54,170
+بنضم صوتنا لصوت القامدي والرازي ونقول إن هذا
+
+295
+00:27:54,170 --> 00:27:58,250
+الخلاف بين الجمهور والحنافية خلافهم إصطلاحي ولا
+
+296
+00:27:58,250 --> 00:28:01,930
+مشح في الإصطلاح كما يقول العلماء لا مشح في
+
+297
+00:28:01,930 --> 00:28:07,830
+الإصطلاح يعنيأيا كان الإصطلاح المهم النتيجة واحدة
+
+298
+00:28:07,830 --> 00:28:12,030
+المهم النتيجة واحدة زي ما بيقولوا all roads lead
+
+299
+00:28:12,030 --> 00:28:17,190
+to Rome يعني كل الطرق تؤدي إلى إيه؟ تؤدي إلى رومة
+
+300
+00:28:17,190 --> 00:28:24,610
+الآن سنتحدث عن أحكام متعلقات الأحكام الشرعية طبعا
+
+301
+00:28:24,610 --> 00:28:32,790
+كل متعلق له حكم هذا الحكم احنا حكينا أقساموأقسام
+
+302
+00:28:32,790 --> 00:28:36,830
+الحكم التكليفي ومتعلقات وهل قد بدنا حكم المتعلق
+
+303
+00:28:36,830 --> 00:28:44,170
+هذا الواجب يلزم الإتيان به ويثاب فاعله ويعاقب
+
+304
+00:28:44,170 --> 00:28:51,450
+تاريخه ويكفر من أنكره إلا عند الحنافية المندوب
+
+305
+00:28:51,450 --> 00:28:58,210
+يلا إيش الحكم المتعلق فيه يستحق فاعله السواب ولا
+
+306
+00:28:58,210 --> 00:29:06,130
+يعاقب تاريخه يعني أناصليت الآن صليت الفرض أو قدّيت
+
+307
+00:29:06,130 --> 00:29:10,150
+الواجب اللي عليا صليت الظهر مثلا أو صليت الفجر
+
+308
+00:29:10,150 --> 00:29:17,290
+الصلاة هذه إيش بده يترتب عليها؟ يعني بده يترتب
+
+309
+00:29:17,290 --> 00:29:21,910
+عليها أول حاجة أنا أنجزتها أتمتها كما طلبها ربنا
+
+310
+00:29:21,910 --> 00:29:27,270
+سبحانه وتعالى الآن لما أنجزتها كما طلبها منه ربنا
+
+311
+00:29:27,270 --> 00:29:31,070
+سبحانه وتعالى الآن الإنجاز هذا إيش بده يترتب عليه؟
+
+312
+00:29:31,720 --> 00:29:38,720
+يساب فاعله ويعاقب تاركه لو تركه ويكفر من أنكره إلا
+
+313
+00:29:38,720 --> 00:29:43,900
+عند الحنافية يعني عند الجمهور بناء على تقسيمات
+
+314
+00:29:43,900 --> 00:29:49,500
+الأحكام الشرعية عند الحنافية المندوب إذا عمل
+
+315
+00:29:49,500 --> 00:29:54,400
+الإنسان شيء مندوب يستحق فاعله ثواب ولا يعاقب تاركه
+
+316
+00:29:54,400 --> 00:29:59,480
+أما الحرام يلزم اجتنابه ويعاقب فاعله ويساب الإنسان
+
+317
+00:29:59,480 --> 00:30:06,140
+على تركهو يكفر مستحيله أما المكروه فاعله لا يستحق
+
+318
+00:30:06,140 --> 00:30:09,980
+العقاب و قد يستحق اللوم و الاعتاب و يساب تاريخه
+
+319
+00:30:09,980 --> 00:30:13,640
+أما المباح لا ثواب ولا عقاب على فعله أو تركه
+
+320
+00:30:13,640 --> 00:30:18,280
+المكروه تحريما يستحق فاعله العقاب و اللوم و لكن لا
+
+321
+00:30:18,280 --> 00:30:24,360
+يكفر منكره عند الحنفية المكروه تنزيها فعله لا
+
+322
+00:30:24,360 --> 00:30:28,980
+يستوجب العقاب ولا الذنب و لكنه يكون خلاف الأولى و
+
+323
+00:30:28,980 --> 00:30:35,810
+الأفضلفيساب تاركه أما الفرض فيلزم الإتيان به ويساب
+
+324
+00:30:35,810 --> 00:30:41,230
+فاعله ويعاقب تاركه ويكفر من أنكره عند الحنفية ولا
+
+325
+00:30:41,230 --> 00:30:48,050
+عند الجمهور؟ عند الكل، عند الكل، تمام؟ إذن هذه هي
+
+326
+00:30:48,050 --> 00:30:54,420
+أحكام متعلقات الأحكام الشرعيةنكتفي بهذا القدر في
+
+327
+00:30:54,420 --> 00:31:00,160
+هذه المحاضرة على أن نقف عند الصيغ التي تعرف بها
+
+328
+00:31:00,160 --> 00:31:05,980
+الأحكام في المحاضرة القادمة نلقاكم على خير السلام
+
+329
+00:31:05,980 --> 00:31:08,360
+عليكم ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/zF8QWIT1WC8.srt b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/zF8QWIT1WC8.srt
new file mode 100644
index 0000000000000000000000000000000000000000..546ae42d57ef9d3600d969a760a7a2a5f5371f32
--- /dev/null
+++ b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/zF8QWIT1WC8.srt
@@ -0,0 +1,775 @@
+1
+00:00:06,440 --> 00:00:09,280
+أعوذ بالله من الشيطان الرجيم بسم الله الرحمن
+
+2
+00:00:09,280 --> 00:00:13,460
+الرحيم إن الحمد لله نحمده و نستعينه و نستغفره و
+
+3
+00:00:13,460 --> 00:00:17,840
+نعوذ بالله من شرور أنفسنا و سيئات أعمالنا  ما يهده 
+
+4
+00:00:17,840 --> 00:00:22,100
+الله فلا مضل له و ما يضلل فلا هادي له و أشهد أن لا
+
+5
+00:00:22,100 --> 00:00:26,840
+إله إلا الله و أشهد أن محمدا عبده و رسوله و بعض
+
+6
+00:00:27,560 --> 00:00:31,820
+بداية أخواتي الحبيبات وبنات الطيبات أحييكم بتحية 
+
+7
+00:00:31,820 --> 00:00:36,420
+طيبة مباركة من عند الله السلام عليكم ورحمة الله 
+
+8
+00:00:36,420 --> 00:00:42,980
+وبركاته سنتحدث اليوم في هذه المحاضرة عن المحكوم
+
+9
+00:00:42,980 --> 00:00:51,660
+فيه أولا المحكوم فيه وهو فعل المكلف طبعا حقيقة
+
+10
+00:00:51,660 --> 00:00:58,520
+المحكوم فيه هو الفعل الذي تعلق به خطاب الشارع زي
+
+11
+00:00:58,520 --> 00:01:05,840
+الإيجاب متعلق الواجب الندب متعلق المندوب التحريم 
+
+12
+00:01:05,840 --> 00:01:12,480
+متعلق الحرام أو المحرم الكراهة ومتعلقها المكروه
+
+13
+00:01:12,480 --> 00:01:18,920
+الإباحة ومتعلقها المباح وسبق أن تحدثنا عن الإيجاب
+
+14
+00:01:18,920 --> 00:01:24,600
+وتعريفه والواجب وتعريفه واستعرضنا الأمثلة التي
+
+15
+00:01:25,120 --> 00:01:30,960
+تتعلق بحقيقة المحكوم فيه أو حقيقة الفعل الذي تعلق
+
+16
+00:01:30,960 --> 00:01:36,220
+به خطاب الشارع وهذا الكلام كمان اللي بدي أحكيه
+
+17
+00:01:36,220 --> 00:01:42,080
+الآن مش جديد لكن خلينا احنا نستعرضه المحكوم فيه
+
+18
+00:01:42,080 --> 00:01:46,720
+إذا كان خطاب الشارع من قبيل الحكم التكليفي فإن
+
+19
+00:01:46,720 --> 00:01:51,440
+المحكوم فيه لا يكون إلا فعلا المكلف زي الصلاة 
+
+20
+00:01:51,440 --> 00:01:58,450
+الصوم، الكتابة، الدين، القتل العمد، العدوان طبعا هذا
+
+21
+00:01:58,450 --> 00:02:03,810
+الكلام له أدلة كثيرة كقول الله تعالى وأقيموا
+
+22
+00:02:03,810 --> 00:02:09,090
+الصلاة واتوا الذكاة فيه طبعا إيجاب تعلق بفعل وهو
+
+23
+00:02:09,090 --> 00:02:14,270
+إقامة الصلاة فجعله واجبا وهي كمان مرة بنقول أن 
+
+24
+00:02:14,270 --> 00:02:20,550
+الكلام هذا مش جديد هذا كلام يعني سبق أن تحدثنا به
+
+25
+00:02:21,310 --> 00:02:24,710
+وقال الله تعالى يا أيها الذين آمنوا إذا تداينتم
+
+26
+00:02:24,710 --> 00:02:30,850
+بدين إلى أجل مسمى، فاكتبوه طبعا فيه ندب
+
+27
+00:02:30,850 --> 00:02:35,770
+تعلق بفعل وهو كتابة الدين فجعله مندوبا فعل مين
+
+28
+00:02:35,770 --> 00:02:42,110
+طبعا فعل المكلف وقوله أيضا وقول الله تعالى ولا 
+
+29
+00:02:42,110 --> 00:02:47,870
+تقتلوا نفس التحريم المستفاد منه تعلق بفعل هو قتل
+
+30
+00:02:47,870 --> 00:02:52,990
+النفس فجعله محرما وقوله عز وجل فإذا قضيت الصلاة
+
+31
+00:02:52,990 --> 00:02:56,830
+فانتشر في الأرض فيه إباحة وهو الانتشار في الأرض
+
+32
+00:02:56,830 --> 00:03:04,030
+وذلك متعلق بفعل المكلف فجعله مباحا أما إذا كان من
+
+33
+00:03:04,030 --> 00:03:08,390
+قبيل الحكم الوضعي فقد يكون المحكم فيه فعل المكلف
+
+34
+00:03:08,390 --> 00:03:13,830
+وقد لا يكون فعل المكلف ولكن له ارتباط بفعله إذا
+
+35
+00:03:13,830 --> 00:03:17,350
+كان المحكم فيه فعل المكلف زي الطهارة التي جعلها
+
+36
+00:03:17,350 --> 00:03:22,930
+المشرع شرطا للصلاة وهي فعل للمكلف مين اللي بده يعمل
+
+37
+00:03:22,930 --> 00:03:25,550
+الطهارة هذه؟ مين اللي بده يتوضى؟ اللي هو مين؟
+
+38
+00:03:25,550 --> 00:03:32,530
+المكلف والزنا الذي جعله الشارع سببا للوجوب طبعا
+
+39
+00:03:32,530 --> 00:03:38,950
+الحد وهو فعل للمكلف قتل الوارث، مورثه الذي جعله
+
+40
+00:03:38,950 --> 00:03:43,050
+الشارع مانع من الميراث وهو فعل للمكلف أيضا البيع
+
+41
+00:03:43,050 --> 00:03:49,950
+جعله الشارع سببا للملكية وهو فعل للمكلف وقد لا يكون
+
+42
+00:03:49,950 --> 00:03:57,730
+فعلا للمكلف ولكن له ارتباط بفعله كدلوق الشمس فإنه
+
+43
+00:03:57,730 --> 00:04:01,350
+ليس فعل من أفعال المكلف ولا نستطيع نطلع الشمس و
+
+44
+00:04:01,350 --> 00:04:05,930
+نرجع الشمس نخليها تشرق و نخليها تغيب، لأ طبعا هذه
+
+45
+00:04:05,930 --> 00:04:11,090
+ليست من أفعال المكلفين لكن مرتبطة بفعل المكلف من
+
+46
+00:04:11,090 --> 00:04:15,550
+ناحية أن الدلوق سبب لوجوب الصلاة التي هي من أفعال
+
+47
+00:04:15,550 --> 00:04:21,730
+المكلف أيضا بلوغ الحلم الذي جعله الشارع شرطا 
+
+48
+00:04:21,730 --> 00:04:26,330
+للتكليف ليس فعلا من أفعال المكلف ولكنه مرتبط بفعل
+
+49
+00:04:26,330 --> 00:04:32,930
+المكلف من ناحية أن بلوغ الحلم سببا لأهلية التكليف
+
+50
+00:04:32,930 --> 00:04:38,270
+وكذلك الحيض والنفاس اللذان جعلهم الشارع مانعين من
+
+51
+00:04:38,270 --> 00:04:43,070
+صحة العبادة من هنا نفهم أنه لا تكليف إلا بفعل كما 
+
+52
+00:04:43,070 --> 00:04:49,330
+يقول علماء الأصول فإذا كان حكم الشارع إيجابا أو
+
+53
+00:04:49,330 --> 00:04:53,730
+ندبا فمتعلق الإيجاب هو فعل الواجب على سبيل الحتم
+
+54
+00:04:53,730 --> 00:04:58,850
+ومتعلق الندب هو فعل المندوب على سبيل الحتم ففي
+
+55
+00:04:58,850 --> 00:05:03,950
+الحالتين يكون تكليفا بفعل وكذلك التحريم والكراهة
+
+56
+00:05:03,950 --> 00:05:09,430
+فالمكلف به فيهما فعل لأنه عبارة عن كف النفس عن
+
+57
+00:05:09,430 --> 00:05:17,580
+الفعل المحرم أو المكروه وضع العلماء شروط للمحكوم
+
+58
+00:05:17,580 --> 00:05:22,960
+فيه خلينا نستعرض هذه الشروط عندنا اشترطوا لصحة
+
+59
+00:05:22,960 --> 00:05:27,620
+التكليف بالفعل ثلاث شروط وهناك شرط رابع مختلف فيه
+
+60
+00:05:27,620 --> 00:05:33,120
+مش هنتكلم عنه أما الشرط الأول يشترط أن يكون الفعل
+
+61
+00:05:33,120 --> 00:05:39,800
+معلوما للمكلف علما تاما حتى يتصور قصده إليه ويؤديه
+
+62
+00:05:39,800 --> 00:05:45,160
+على الوجه المطلوب منه فلا يكلف الشخص بالصلاة حتى 
+
+63
+00:05:45,160 --> 00:05:49,820
+يعرف أركان الصلاة وشروط الصلاة وكيفيّة أداء الصلاة
+
+64
+00:05:49,820 --> 00:05:54,580
+حيث أن الأمر
+
+65
+00:05:54,580 --> 00:06:00,460
+بالصلاة في القرآن جاء مجملا قال تعالى وأقيموا 
+
+66
+00:06:00,460 --> 00:06:04,300
+الصلاة ومن الذي بين هذا المجمل؟ النبي صلى الله 
+
+67
+00:06:04,300 --> 00:06:10,820
+عليه وسلم بينه بفعله في الصلاة كما قال للصحابة رضي
+
+68
+00:06:10,820 --> 00:06:15,830
+الله عنهم صلوا كما رأيتموني و صليت وهيك علمهم و
+
+69
+00:06:15,830 --> 00:06:21,390
+صارت الصلاة معلومة لديهم من حيث أركانها وشروطها و
+
+70
+00:06:21,390 --> 00:06:28,210
+كيفيتها وكذلك الزكاة والصوم والحج فكل هذه أمور
+
+71
+00:06:28,210 --> 00:06:32,990
+جاءت مجملة والنبي صلى الله عليه وسلم بين حقيقتها
+
+72
+00:06:32,990 --> 00:06:38,190
+وأركانها وشروطها ومقدارها ووقتها حتى أصبحت
+
+73
+00:06:38,190 --> 00:06:44,310
+معلومة للمكلف علما تاما إذن شروط المحكم فيه أن
+
+74
+00:06:44,310 --> 00:06:50,850
+يكون الفعل معلوما للمكلف علما تاما حتى يتصور قصده
+
+75
+00:06:50,850 --> 00:06:55,550
+إليه ويؤديه على الوجه المطلوب مش معقول واحد حاجة
+
+76
+00:06:55,550 --> 00:07:00,590
+ما يعرفهاش ييجي تقوله اعملها ويقدر ينفذها لازم يكون
+
+77
+00:07:00,590 --> 00:07:06,830
+يتوضح له هذا الأمر زي ما النبي صلى الله عليه وسلم
+
+78
+00:07:06,830 --> 00:07:12,250
+فعل مع الصحابة وضح لهم كيفية الصلاة وأركان الصلاة
+
+79
+00:07:12,250 --> 00:07:19,050
+وشروطها وكيفية أداءها وبعد ذلك طالبهم بالصلاة
+
+80
+00:07:19,050 --> 00:07:26,210
+السؤال الآن ما المراد بعلم المكلف بما كلف به؟ طبعا
+
+81
+00:07:26,210 --> 00:07:32,630
+هنا المراد هو إمكان علمه به وليس المراد علمه به
+
+82
+00:07:32,630 --> 00:07:39,320
+فعلا إيش يعني؟ يعني الإنسان الذي يصل إلى سن البلوغ
+
+83
+00:07:39,320 --> 00:07:45,320
+هو عاقل قادر على معرفة الأحكام الشرعية بنفسه وإذا
+
+84
+00:07:45,320 --> 00:07:50,580
+مش قادر هو بنفسه يعرف الأحكام هذه، بيتوجه للعلماء
+
+85
+00:07:50,580 --> 00:07:56,900
+بالسؤال هنا يعتبر عالمًا بما كلف به وتنفذ عليه
+
+86
+00:07:56,900 --> 00:08:02,780
+الأحكام ويلزم بآثارها ولا يقبل منه الاعتذار بالجهل
+
+87
+00:08:03,200 --> 00:08:07,760
+يعني مينفعش واحد نقوله صلي يقول بعرفش أصلي لأ بدك
+
+88
+00:08:07,760 --> 00:08:13,580
+تروح تقرأ عن الصلاة كيف أحكامها وتتعلمها بنفسك و
+
+89
+00:08:13,580 --> 00:08:18,160
+مش تعرفهاش من حالك بتروح إيش بتتوجه للعلماء و
+
+90
+00:08:18,160 --> 00:08:21,900
+بتسألهم عشان هيك قال الفقهاء لا يقبل في دار
+
+91
+00:08:21,900 --> 00:08:26,780
+الإسلام عذر بالجهل للحكم الشرعي وبهذا الإمكان
+
+92
+00:08:26,780 --> 00:08:32,340
+يستطيع المكلف القيام بما كلف به السبب في الاكتفاء
+
+93
+00:08:32,340 --> 00:08:37,520
+بإمكان العلم بالحكم هو أنه لو شرطه لصحة التكليف
+
+94
+00:08:37,520 --> 00:08:42,180
+علم المكلف بالفعل لا إمكانه لتسعى طبعا مجال
+
+95
+00:08:42,180 --> 00:08:47,220
+الاعتذار بجهل الأحكام ولا استقامة التكليف وفي هذا
+
+96
+00:08:47,220 --> 00:08:54,940
+تعطيل للأحكام أما بالنسبة للشرط الثاني طبعا احنا
+
+97
+00:08:54,940 --> 00:08:59,570
+عشان نذكركم احنا بنتكلم في شروط المحكم فيه خلصنا
+
+98
+00:08:59,570 --> 00:09:04,770
+الشرط الأول، ننتقل الآن إلى الشرط الثاني أن يعلم
+
+99
+00:09:04,770 --> 00:09:10,810
+المكلف طلب الله تعالى للفعل حتى يعتبر طاعة وامتثال
+
+100
+00:09:10,810 --> 00:09:15,430
+لأوامر الله تعالى علشان هيك المسلم لا يقبل حكما
+
+101
+00:09:15,430 --> 00:09:19,950
+إلا إذا علم أن الله سبحانه وتعالى قد طلبه ليتصور
+
+102
+00:09:19,950 --> 00:09:25,430
+قصد الطاعة وامتثال لله تعالى فيه أما هذا طبعا
+
+103
+00:09:25,430 --> 00:09:30,970
+الشرط واضح، ما فيهوش شرح كتير أما الشرط الثالث
+
+104
+00:09:30,970 --> 00:09:36,670
+أن يكون الفعل المكلف به ممكن بحيث يستطيع المكلف أن
+
+105
+00:09:36,670 --> 00:09:41,970
+يفعله أو يتركه إيش يعني أن يكون الفعل المكلف به
+
+106
+00:09:41,970 --> 00:09:48,610
+ممكن يعني يستطيع المكلف فعله بدون مشقة يعني لو 
+
+107
+00:09:48,610 --> 00:09:56,050
+قلنا إن صلاة الفجر لازم نصليها على سور الحيط تمام؟
+
+108
+00:09:56,380 --> 00:10:00,760
+كم واحد حيصلي على سور الحيط صلاة الفجر؟ قليل،
+
+109
+00:10:00,760 --> 00:10:04,940
+واللي حيصلي يوم عن يوم هيتعب، هيبطل يصلي، إذا
+
+110
+00:10:04,940 --> 00:10:08,940
+عشان هيك، بدو يكون الفعل إيه؟ بدو يكون ممكن، يعني
+
+111
+00:10:08,940 --> 00:10:13,860
+يستطيع المكلف أن ينفذه بدون كلفة، بدون مشقة وبدون
+
+112
+00:10:13,860 --> 00:10:20,460
+تعب طبعا، مجال التكليف الشرعي للأفعال التي لا تخرج
+
+113
+00:10:20,460 --> 00:10:27,290
+عن طاقة المكلفين، طاقة المكلفين و وسعهم وما ليس فيه
+
+114
+00:10:27,290 --> 00:10:31,450
+من المشقة والعناء ما يخرج عن المعهود في الأعمال
+
+115
+00:10:31,450 --> 00:10:35,790
+العادية طبعا لما ربنا سبحانه وتعالى يطلب مننا معنى
+
+116
+00:10:35,790 --> 00:10:40,890
+الكلام هذا يطلب مننا الصلاة صلاة سهلة أني أنا كل
+
+117
+00:10:40,890 --> 00:10:46,270
+ما علي أني أطهر للصلاة هذه وأبدأ في فعل الصلاة
+
+118
+00:10:46,270 --> 00:10:52,950
+مرات بتقول يمكن هذا الفعل فيه مشقة اه المشقة
+
+119
+00:10:52,950 --> 00:10:59,130
+نوعين مشقة ممكنة ومشقة غير ممكنة مشقة ممكنة يعني
+
+120
+00:10:59,130 --> 00:11:05,110
+يستطيع المكلف أن يفعلها لكن مشقة غير ممكنة هذه لا
+
+121
+00:11:05,110 --> 00:11:10,830
+يستطيع المكلف أن يفعلها وبالتالي سيترك هذه العبادة
+
+122
+00:11:10,830 --> 00:11:17,410
+لكن المشرع يعني أن المكلف يقوم بالعبادة وينفذ
+
+123
+00:11:17,410 --> 00:11:22,890
+العبادة حتى يقوم بإعمار الأرض طبعا يمكن الفعل يكون
+
+124
+00:11:22,890 --> 00:11:28,680
+ثقيل على النفس إن بدي أصحى من النوم وأتوضى وأصلي
+
+125
+00:11:28,680 --> 00:11:33,640
+هذا فعل يعني ممكن يكون أي فعل يصير ممكن يكون ثقيل
+
+126
+00:11:33,640 --> 00:11:41,280
+على النفس لكن هنا وإن أحس المكلف بعبق لم يكن يحس 
+
+127
+00:11:41,280 --> 00:11:46,040
+به قبل التكليف هذا الشعور بنسميه شعور فطري لا يمكن
+
+128
+00:11:46,040 --> 00:11:52,480
+رفعه شعور فطري يعني كل واحد ممكن يتعب من أنه أو يجد
+
+129
+00:11:52,480 --> 00:11:58,980
+مشقة من أنه يقوم للصلاة ويتوضى ويصلي لكن هذا
+
+130
+00:11:58,980 --> 00:12:04,480
+الشعور المشقة بسيط ويمكن يتغلب عليه الإنسان وهو
+
+131
+00:12:04,480 --> 00:12:10,990
+شعور فطري لا يمكن يعني إزالته من نفس الإنسان إن نفس
+
+132
+00:12:10,990 --> 00:12:16,990
+الإنسان حب الإنسان الراحة والخلود إلى يعني العدم
+
+133
+00:12:16,990 --> 00:12:24,290
+القيم من النوم وعدم فعل أي أمر يسبب له تعب هذا
+
+134
+00:12:24,290 --> 00:12:29,450
+التعب وإن كان بسيط لكن الشارع يعلم أن المكلف ممكن
+
+135
+00:12:29,450 --> 00:12:33,290
+أن يتغلب على هذا التعب وعلى هذا الشعور الفطري
+
+136
+00:12:33,290 --> 00:12:39,750
+ويحقق العبادة هنا عندنا بالنسبة لأقسام المحكم فيه
+
+137
+00:12:39,750 --> 00:12:44,290
+مش هناخده بالتفصيل لكن هنستعرضه استعراض الحنفية
+
+138
+00:12:44,290 --> 00:12:49,090
+قسم وفعل المكلف من حيث صلته بأحكام الله تعالى
+
+139
+00:12:49,090 --> 00:12:53,990
+وحقوق العباد إلى أربع أقسام حق خالص لله تعالى، حق
+
+140
+00:12:53,990 --> 00:12:58,830
+خالص للعبد، مجتمع فيه الحقان وحق الله غالب، مجتمع
+
+141
+00:12:58,830 --> 00:13:03,800
+فيه الحقان وحق العبد غالبهذه تقسيم الحقوق أو تقسيم
+
+142
+00:13:03,800 --> 00:13:09,460
+الأقسام المحكوم فيها عند الحنفية بس يعني اعرفوهم 
+
+143
+00:13:09,460 --> 00:13:14,520
+معرفة بدون تفصيل بدون تعريف لكل حق من الحقوق هذه
+
+144
+00:13:14,520 --> 00:13:20,600
+بدون أن نستعرض أمثلة على هذه الحقوق هذا بس للإعلان 
+
+145
+00:13:20,600 --> 00:13:25,000
+فقط الآن
+
+146
+00:13:25,000 --> 00:13:31,560
+سنتحدث عن المحكوم عليه المحكوم عليه الآن هو المكلف
+
+147
+00:13:32,040 --> 00:13:38,220
+ومن هنا بدنا نبدأ طبعا الكلام المهم الذي يتعلق
+
+148
+00:13:38,220 --> 00:13:44,640
+بالمكلف المكلف هو الشخص البالغ العقل الذي تعلق خطاب
+
+149
+00:13:44,640 --> 00:13:51,060
+الشارع بفعله ووجه إليه الأحكام وطالبه بتنفيذها شرط
+
+150
+00:13:51,060 --> 00:13:56,120
+صحة التكليف أو شرط صحة التكليف المكلف طبعا عندي
+
+151
+00:13:56,120 --> 00:14:00,400
+شرطين ومهمين هذول الشرطين الشرط الأول اللي هو 
+
+152
+00:14:00,400 --> 00:14:04,320
+العقل والفهم والشرط الثاني اللي هو الأهلية اللي
+
+153
+00:14:04,320 --> 00:14:09,820
+هنتكلم عنها إن شاء الله بتفصيل كبير في الفصل
+
+154
+00:14:09,820 --> 00:14:15,100
+الأخير اللي هو الفصل الثالث إن شاء الله تعالى أما
+
+155
+00:14:15,100 --> 00:14:19,040
+بالنسبة ليه الشرط الأول العقل والفهم؟ يعني أن يكون
+
+156
+00:14:19,040 --> 00:14:23,360
+المكلف قادرا على فهم دليل التكليف بأن يفهم الكلام
+
+157
+00:14:23,360 --> 00:14:27,140
+الذي وجهه إليه والمراد بإن الفهم استطاعة فهم
+
+158
+00:14:27,140 --> 00:14:32,600
+المكلف للخطاب وتصوره وليس تصديق به عشان هيك بنقول
+
+159
+00:14:32,600 --> 00:14:37,080
+الكفار مخاطبون بالإيمان وإن لم يقع منهم تصديق
+
+160
+00:14:37,080 --> 00:14:40,580
+باتفاق العلماء لأن الرسول صلى الله عليه وسلم مبعوث
+
+161
+00:14:40,580 --> 00:14:44,960
+إلى جميع الخلق عشان هيك يقول أحد علماء الأصول وهو
+
+162
+00:14:44,960 --> 00:14:49,300
+الشوكاني لا خلافة في أنهم مخاطبون يعني الكفار بأمر
+
+163
+00:14:49,300 --> 00:14:53,080
+الإيمان لأنه النبي صلى الله عليه وسلم مبعوث إلى
+
+164
+00:14:53,080 --> 00:14:59,720
+الكافة وبالمعاملات أيضا دليل هذا الشرط لزوم المحال
+
+165
+00:14:59,720 --> 00:15:05,480
+عند عدم اعتباره في التكليف كيف؟ لأن طلب حصول الفعل
+
+166
+00:15:05,480 --> 00:15:09,960
+من المكلف يقصد به الامتثال والابتلاء وذلك مستحيل
+
+167
+00:15:09,960 --> 00:15:15,640
+عادة وشرعا ممن لا شعور له ولأنه لو صح ذلك التكليف
+
+168
+00:15:15,640 --> 00:15:20,520
+لكل فترة البهائم التي حالة عدم الفهم بينها وبين
+
+169
+00:15:20,520 --> 00:15:26,630
+الخطاب يعني لابد أن يوجد العقل والفهم حتى يتم تكليف
+
+170
+00:15:26,630 --> 00:15:29,970
+المكلف بترتب على هذا الشرط عدة أمور هذه الأمور
+
+171
+00:15:29,970 --> 00:15:33,650
+اللي هنستعرضها سريعا لها تفصيل في الحديث عن 
+
+172
+00:15:33,650 --> 00:15:39,650
+الأهلية عدم تكليف طبعا المجنون والصبي هذا شيء يعني
+
+173
+00:15:39,650 --> 00:15:46,550
+بديهي كيف نكلف من لا عقل له ومن لا إدراك له وهذا
+
+174
+00:15:46,550 --> 00:15:50,890
+هنتكلم إن شاء الله بالتفصيل عنه عدم تكليف النائم
+
+175
+00:15:50,890 --> 00:15:56,300
+والغافل والسكران برضه لنا حديث طويل عنهم طبعا عدم
+
+176
+00:15:56,300 --> 00:16:02,480
+تكليفهم بالعبادة لكن النفقة للأقارب والزكاة وجوب
+
+177
+00:16:02,480 --> 00:16:05,940
+الضمان فيما يتلفه الصبي والمجنون ليس من خطاب
+
+178
+00:16:05,940 --> 00:16:09,900
+التكليف إنما من خطاب الوضع اللي هو ربط الأسباب
+
+179
+00:16:09,900 --> 00:16:14,140
+بالمسببات لأن هذه الواجبات لم تتعلق بفعل الصبي
+
+180
+00:16:14,140 --> 00:16:19,000
+والمجنون إنما تعلقت بالمال أو الذمة وهذا برضه
+
+181
+00:16:19,000 --> 00:16:23,670
+هنوضحه أكثر لما نتكلم عن أهلية الصبي وأهلية المجنون
+
+182
+00:16:23,670 --> 00:16:30,810
+ليش؟ لأنهم صالحان بما ثبت لهم من إنسانية تهيئتهم
+
+183
+00:16:30,810 --> 00:16:36,310
+لفهم الخطاب عند حدوث البلوغ والإفاقة ومتول الأداء
+
+184
+00:16:36,310 --> 00:16:42,550
+هو الولي بعد بلوغ الصبي وإفاقة المجنون كمان مرة
+
+185
+00:16:42,550 --> 00:16:48,690
+بقول إن هذا الكلام هنقوم بتفصيله إن شاء الله عندها
+
+186
+00:16:48,690 --> 00:16:52,730
+في الفصل الثالث وهو الأخير معناه بيكون إن شاء الله
+
+187
+00:16:52,730 --> 00:16:57,170
+الشرط الثاني الأهلية طبعاً أن يكون المكلف أهلا
+
+188
+00:16:57,170 --> 00:17:01,330
+للتكليف وأهلية المكلف لا تتحقق إلا بالعقل والفهم
+
+189
+00:17:01,330 --> 00:17:07,550
+لأن العقل شرط للتكليف برضه موضوع الأهلية إن شاء
+
+190
+00:17:07,550 --> 00:17:13,740
+الله نفصل الحديث فيه بتعريف الأهلية وسنتكلم عن
+
+191
+00:17:13,740 --> 00:17:19,060
+عوارض الأهلية السماوية والمكتسبة بإذن الله تعالى
+
+192
+00:17:19,060 --> 00:17:25,140
+كما قلنا في الفصل الثالث بإذن الله تعالى نكتفي
+
+193
+00:17:25,140 --> 00:17:29,740
+بهذا القدر في هذه المحاضرة على أن نلقاكم في
+
+194
+00:17:29,740 --> 00:17:34,180
+المحاضرة القادمة السلام عليكم ورحمة الله وبركاته 
diff --git a/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/zF8QWIT1WC8_postprocess.srt b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/zF8QWIT1WC8_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..a934655cf4dd798b5b9c6f76f9635f6380e386b6
--- /dev/null
+++ b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/zF8QWIT1WC8_postprocess.srt
@@ -0,0 +1,776 @@
+1
+00:00:06,440 --> 00:00:09,280
+أعوذ بالله من الشيطان الرجيم بسم الله الرحمن
+
+2
+00:00:09,280 --> 00:00:13,460
+الرحيم إن الحمد لله نحمده و نستعينه و نستغفره و
+
+3
+00:00:13,460 --> 00:00:17,840
+نعوذ بالله من شرور أنفسنا و سيئات أعمالنا ما يهده
+
+4
+00:00:17,840 --> 00:00:22,100
+الله فلا مضل له و ما يضلل فلا هادي له و أشهد أن لا
+
+5
+00:00:22,100 --> 00:00:26,840
+إله إلا الله و أشهد أن محمدا عبده و رسوله و بعض
+
+6
+00:00:27,560 --> 00:00:31,820
+بداية أخوات الحبيبات وبنات الطيبات أحييكم بتحية
+
+7
+00:00:31,820 --> 00:00:36,420
+طيبة مباركة من عند الله السلام عليكم ورحمة الله
+
+8
+00:00:36,420 --> 00:00:42,980
+وبركاته سنتحدث اليوم في هذه المحاضرة عن المحكوم
+
+9
+00:00:42,980 --> 00:00:51,660
+فيه أولا المحكوم فيه وهو فعل المكلف طبعا حقيقة
+
+10
+00:00:51,660 --> 00:00:58,520
+المحكوم فيه هو الفعل الذي تعلقبه خطاب الشارع زي
+
+11
+00:00:58,520 --> 00:01:05,840
+الإجاب متعلق الواجب الندب متعلق المندوب التحريم
+
+12
+00:01:05,840 --> 00:01:12,480
+متعلق الحرام أو المحرم الكراهة ومتعلقها المكروه
+
+13
+00:01:12,480 --> 00:01:18,920
+الإباحة ومتعلقها المباح وسبق أن تحدثنا عن الإجاب
+
+14
+00:01:18,920 --> 00:01:24,600
+وتعريفه والواجب وتعريفه واستعرضنا الأمثلة التي
+
+15
+00:01:25,120 --> 00:01:30,960
+تتعلق بحقيقة المحكوم فيه أو حقيقة الفعل الذي تعلق
+
+16
+00:01:30,960 --> 00:01:36,220
+به خطاب الشارع وهذا الكلام كمان اللي بدي أحكيه
+
+17
+00:01:36,220 --> 00:01:42,080
+الآن مش جديد لكن خلّينا احنا نستعرضه المحكوم فيه
+
+18
+00:01:42,080 --> 00:01:46,720
+إذا كان خطاب الشارع من قبيل الحكم التكليفي فإن
+
+19
+00:01:46,720 --> 00:01:51,440
+المحكوم فيه لا يكون إلا فعلا المكلف زي الصلاة
+
+20
+00:01:51,440 --> 00:01:58,450
+الصومكتابة الدين القتل العمد العدوان طبعا هذا
+
+21
+00:01:58,450 --> 00:02:03,810
+الكلام له أدلة كثيرة كقول الله تعالى وأقيموا
+
+22
+00:02:03,810 --> 00:02:09,090
+الصلاة واتوا الذكاء فيه طبعا إيجاب تعلق بفعل وهو
+
+23
+00:02:09,090 --> 00:02:14,270
+إقامة الصلاة فجعله واجبا وهي كمان مرة بنقول أن
+
+24
+00:02:14,270 --> 00:02:20,550
+الكلام هذا مش جديد هذا كلام يعني سبق أن تحدثنا به
+
+25
+00:02:21,310 --> 00:02:24,710
+وقال الله تعالى يا أيها الذين آمنوا إذا تدايمتم
+
+26
+00:02:24,710 --> 00:02:30,850
+بدين إلى أجل المسمى، مسما فاكتبوه طبعا فيه ندب
+
+27
+00:02:30,850 --> 00:02:35,770
+تعلق بفعل وهو كتابة الدين فجعله مندوبا فعل مين
+
+28
+00:02:35,770 --> 00:02:42,110
+طبعا فعل المكلف وقوله أيضا وقول الله تعالى ولا
+
+29
+00:02:42,110 --> 00:02:47,870
+تقتلوا نفس التحريم المستفاد منه تعلق بفعل هو قتل
+
+30
+00:02:47,870 --> 00:02:52,990
+النفس فجعله محرماوقوله عز وجل فإذا قضيت الصلاة
+
+31
+00:02:52,990 --> 00:02:56,830
+فانتشره في الأرض فيه إباحة وهو الانتشار في الأرض
+
+32
+00:02:56,830 --> 00:03:04,030
+وذلك متعلق بفعل المكلف فجعله مباحا أما إذا كان من
+
+33
+00:03:04,030 --> 00:03:08,390
+قبيل الحكم الوضعي فقد يكون المحكم فيه فعل المكلف
+
+34
+00:03:08,390 --> 00:03:13,830
+وقد لا يكون فعل المكلف ولكن له ارتباط بفعله إذا
+
+35
+00:03:13,830 --> 00:03:17,350
+كان المحكم فيه فعل المكلف زي الطهارة التي جعلها
+
+36
+00:03:17,350 --> 00:03:22,930
+المشرع شرطا للصلاةوهي فعل للمكلف مين اللي بده يعمل
+
+37
+00:03:22,930 --> 00:03:25,550
+التهارة هذه؟ مين اللي بده يتوضى؟ اللي هو مين؟
+
+38
+00:03:25,550 --> 00:03:32,530
+المكلف والزنا الذي جعله الشارع سببا للوجوب طبعا
+
+39
+00:03:32,530 --> 00:03:38,950
+الحد وهو فعل للمكلف قتل الوارث مورسه الذي جعله
+
+40
+00:03:38,950 --> 00:03:43,050
+الشارع مانع من الميراث وهو فعل للمكلف أيضا البيع
+
+41
+00:03:43,050 --> 00:03:49,950
+جعله الشارع سببا للملكية وهو فعل للمكلفوقد لا يكون
+
+42
+00:03:49,950 --> 00:03:57,730
+فعلًا للمكلف ولكن له ارتباط بفعله كدلوق الشمس فإنه
+
+43
+00:03:57,730 --> 00:04:01,350
+ليس فعل من أفعال المكلف و لا نستطيع نطلع الشمس و
+
+44
+00:04:01,350 --> 00:04:05,930
+نرجع الشمس نخليها تشرق و نخليها تغيب، لأ طبعًا هذه
+
+45
+00:04:05,930 --> 00:04:11,090
+ليست من أفعال المكلفين لكن مرتبطة بفعل المكلف من
+
+46
+00:04:11,090 --> 00:04:15,550
+ناحية أن الدلوق سبب لوجوب الصلاة التي هي من أفعال
+
+47
+00:04:15,550 --> 00:04:21,730
+المكلفأيضًا بلوغ الحلم الذي جعله الشارع شرطان
+
+48
+00:04:21,730 --> 00:04:26,330
+للتكليف ليس فعلا من أفعال المكلف ولكنه مرتبط بفعل
+
+49
+00:04:26,330 --> 00:04:32,930
+المكلف من ناحية أن بلوغ الحلم سببًا لأهلية التكليف
+
+50
+00:04:32,930 --> 00:04:38,270
+وكذلك الحيد والنفاس اللذان جعلهم الشارع مانعين من
+
+51
+00:04:38,270 --> 00:04:43,070
+صحة العبادة من هنا نفهم أنه لا تكليف إلا بفعل كما
+
+52
+00:04:43,070 --> 00:04:49,330
+يقول علماء الأصولفإذا كان حكم الشارع إيجابا أو
+
+53
+00:04:49,330 --> 00:04:53,730
+ندبا فمتعلق الإيجاب هو فعل الواجب على سبيل الحتم
+
+54
+00:04:53,730 --> 00:04:58,850
+ومتعلق الندب هو فعل المندوب لعل سبيل الحتم ففي
+
+55
+00:04:58,850 --> 00:05:03,950
+الحالتين يكون تكليفا بفعل وكذلك التحريم والكرهة
+
+56
+00:05:03,950 --> 00:05:09,430
+فالمكلف بيه فيهما فعل لأنه عبارة عن كف النفس عن
+
+57
+00:05:09,430 --> 00:05:17,580
+الفعل المحرم أو ال macroوضع العلماء شروط للمحكوم
+
+58
+00:05:17,580 --> 00:05:22,960
+فيه خلّينا نستعرض هذه الشروط عندنا اشترطوا لصحة
+
+59
+00:05:22,960 --> 00:05:27,620
+التكليف بالفعل تلت شروط وهناك شرط رابع مختلف فيه
+
+60
+00:05:27,620 --> 00:05:33,120
+مش هنتكلم عنه أما الشرط الأول يشترط أن يكون الفعل
+
+61
+00:05:33,120 --> 00:05:39,800
+معلوما للمبلغ علما داما حتى يتصور قصده إليه ويؤديه
+
+62
+00:05:39,800 --> 00:05:45,160
+على الوجه المطلوب منهفلا يكلف الشخص بالصلاة حتى
+
+63
+00:05:45,160 --> 00:05:49,820
+يعرف أركان الصلاة وشروط الصلاة وكيفيت أدا الصلاة
+
+64
+00:05:49,820 --> 00:05:54,580
+حيث أن الأمر
+
+65
+00:05:54,580 --> 00:06:00,460
+بالصلاة في القرآن جاء مجملا قال تعالى وأقيموا
+
+66
+00:06:00,460 --> 00:06:04,300
+الصلاة ومن الذي بين هذا المجمل؟ النبي صلى الله
+
+67
+00:06:04,300 --> 00:06:10,820
+عليه وسلم بينه بفعله في الصلاة كما قال للصحابة رضي
+
+68
+00:06:10,820 --> 00:06:15,830
+الله عنهمصلوا كما رأيتموني و صليت و هيك علمهم و
+
+69
+00:06:15,830 --> 00:06:21,390
+صارت الصلاة معلومة لديهم من حيث أركانها و شروطها و
+
+70
+00:06:21,390 --> 00:06:28,210
+كيفيتها و كذلك الذكاء و الصوم و الحج فكل هذه أمور
+
+71
+00:06:28,210 --> 00:06:32,990
+جاءت مجملة و النبي صلى الله عليه و سلم بين حقيقتها
+
+72
+00:06:32,990 --> 00:06:38,190
+و أركانها و شروطها و مقدارها و وقتها حتى أصبحت
+
+73
+00:06:38,190 --> 00:06:44,310
+معلومة للمكلف علما تمامإذا شروط المحكم فيها أن
+
+74
+00:06:44,310 --> 00:06:50,850
+يكون الفعل معلوما للمكلف علما تاما حتى يتصور قصده
+
+75
+00:06:50,850 --> 00:06:55,550
+إليه ويؤديه على الوجه المطلوب مش معقول واحد حاجة
+
+76
+00:06:55,550 --> 00:07:00,590
+ما بعرفاش ييجي تقوله اعملها ويقدر ينفذها لازم يكون
+
+77
+00:07:00,590 --> 00:07:06,830
+يتوضح له هذا الأمروزي ما النبي صلى الله عليه وسلم
+
+78
+00:07:06,830 --> 00:07:12,250
+فعل مع الصحابة وضح لهم كيفية الصلاة وأركان الصلاة
+
+79
+00:07:12,250 --> 00:07:19,050
+وشروطها وكيفية أداءه وبعد ذلك طالبهم بالصلاة
+
+80
+00:07:19,050 --> 00:07:26,210
+السؤال الآن ما المراد بعلم المكلف بما كلف به؟ طبعا
+
+81
+00:07:26,210 --> 00:07:32,630
+هنا المراد هو يمكن علمه به وليس المراد علمه به
+
+82
+00:07:32,630 --> 00:07:39,320
+فعلاإيش يعني؟ يعني الإنسان الذي يصل إلى سن البلوغ
+
+83
+00:07:39,320 --> 00:07:45,320
+هو عاقل قادر على معرفة الأحكام الشرعية بنفسه وإذا
+
+84
+00:07:45,320 --> 00:07:50,580
+مش قادر هو بنفسه يعرف الأحكام هذه، متوجه للعلماء
+
+85
+00:07:50,580 --> 00:07:56,900
+بالسؤال هنا يعتبر عالمًا بما كلف به وتنفذ عليه
+
+86
+00:07:56,900 --> 00:08:02,780
+الأحكام ويلزم بآثارها ولا يقبل منه الاعتذار بالجهل
+
+87
+00:08:03,200 --> 00:08:07,760
+يعني مينفعش واحد نقوله صلي يقول بعرفش أصلي لأ بدك
+
+88
+00:08:07,760 --> 00:08:13,580
+تروح تقرا عن الصلاة كيف أحكامها و تتعلمها بنفسك و
+
+89
+00:08:13,580 --> 00:08:18,160
+مش تعرفش من حالك بتروح إيش بتتوجه للعلماء و
+
+90
+00:08:18,160 --> 00:08:21,900
+بتسألهم عشان هيك قال الفقهاء لا يقبل في دار
+
+91
+00:08:21,900 --> 00:08:26,780
+الإسلام عذر بالجهل للحكم الشرعي وبهذا الإمكان
+
+92
+00:08:26,780 --> 00:08:32,340
+يستطيع المكلف القيام بما كلف بهوالسبب في الاكتفاء
+
+93
+00:08:32,340 --> 00:08:37,520
+بإمكان العلم بالحكم هو أنه لو شرطه لصحة التكليف
+
+94
+00:08:37,520 --> 00:08:42,180
+علم المكلف بالفعل لا إمكانه لتسعى طبعا مجال
+
+95
+00:08:42,180 --> 00:08:47,220
+الاعتذار بجهل الأحكام ولا مستقامة التكليف وفي هذا
+
+96
+00:08:47,220 --> 00:08:54,940
+تعطيل للأحكم أما بالنسبة للشرط التاني طبعا احنا
+
+97
+00:08:54,940 --> 00:08:59,570
+عشان نذكركم احنا بنتكلم في شروط المحكم فيهخلصنا
+
+98
+00:08:59,570 --> 00:09:04,770
+الشرط الأول، ننتقل الآن إلى الشرط الثاني أن يعلم
+
+99
+00:09:04,770 --> 00:09:10,810
+المكلف طلب الله تعالى للفعل حتى يعتبر طاعة وامتثال
+
+100
+00:09:10,810 --> 00:09:15,430
+لأوامر الله تعالى علشان هيك المسلم لا يقبل حكما
+
+101
+00:09:15,430 --> 00:09:19,950
+إلا إذا علم أن الله سبحانه وتعالى قد طلبه ليتصور
+
+102
+00:09:19,950 --> 00:09:25,430
+قصد الطاعة وامتثال لله تعالى فيه أما هذا طبعا
+
+103
+00:09:25,430 --> 00:09:30,970
+الشرط واضح، مافيدهشيعني شرح كتير أما الشرط التالث
+
+104
+00:09:30,970 --> 00:09:36,670
+أن يكون الفعل المكلف به ممكن بحيث يستطيع المكلف أن
+
+105
+00:09:36,670 --> 00:09:41,970
+يفعله أو يتركه إيش يعني أن يكون الفعل المكلف به
+
+106
+00:09:41,970 --> 00:09:48,610
+ممكن يعني يستطيع المكلف فعله بدون مشقة يعني لو
+
+107
+00:09:48,610 --> 00:09:56,050
+قلنا إن صلاة الفجر لازم نصليها على صور الحيط تمام؟
+
+108
+00:09:56,380 --> 00:10:00,760
+أكم واحد حيصلّي على سور الحيط صلاة الفجر؟ قليل،
+
+109
+00:10:00,760 --> 00:10:04,940
+واللي حيصلّي يوم عن يوم هيتعب، هيبطل يصلّي، إذا
+
+110
+00:10:04,940 --> 00:10:08,940
+عشان هيك، بدو يكون الفعل إيه؟ بدو يكون ممكن، يعني
+
+111
+00:10:08,940 --> 00:10:13,860
+يستطيع المكلف أن ينفذه بدون كلفة، بدون مشقة وبدون
+
+112
+00:10:13,860 --> 00:10:20,460
+تعب طبعا، مجال التكليف الشرعي للأفعال التي لتخرج
+
+113
+00:10:20,460 --> 00:10:27,290
+عن طاعة المكلفين، طاقة المكلفين ووصهموما ليس فيه
+
+114
+00:10:27,290 --> 00:10:31,450
+من المشقة والعناء ما يخرج عن المعهود في الأعمال
+
+115
+00:10:31,450 --> 00:10:35,790
+العادية طبعا لما ربنا سبحانه وتعالى يطلب مننا معنى
+
+116
+00:10:35,790 --> 00:10:40,890
+الكلام هذا يطلب مننا الصلاة صلاة سهلة أني أنا كل
+
+117
+00:10:40,890 --> 00:10:46,270
+ما علي أني أطهر للصلاة هذه و أبدأ في فعل الصلاة
+
+118
+00:10:46,270 --> 00:10:52,950
+مرات بتقول يمكن هذا الفعل تقيل وفي مشقة اه المشقة
+
+119
+00:10:52,950 --> 00:10:59,130
+نوعين مشقةممكنة ومشقة غير ممكنة مشقة ممكنة يعني
+
+120
+00:10:59,130 --> 00:11:05,110
+يستطيع المكلف أن يفعلها لكن مشقة غير ممكنة هذه لا
+
+121
+00:11:05,110 --> 00:11:10,830
+يستطيع المكلف أن يفعلها وبالتالي سيترك هذه العبادة
+
+122
+00:11:10,830 --> 00:11:17,410
+لكن المشرع يعني أن المكلف يقوم بالعبادة وينفذ
+
+123
+00:11:17,410 --> 00:11:22,890
+العبادة حتى يقوم بإعمار الأرض طبعا يمكن الفعل يكون
+
+124
+00:11:22,890 --> 00:11:28,680
+ثقيل على النفسإن بدي أصحى من النوم و أتودى و أصلي
+
+125
+00:11:28,680 --> 00:11:33,640
+هذا فعل يعني ممكن يكون أي فعل يصير ممكن يكون ثقيل
+
+126
+00:11:33,640 --> 00:11:41,280
+على النفس لكن هنا وإن أحس المكلف بعبق لم يكن يحس
+
+127
+00:11:41,280 --> 00:11:46,040
+به قبل التكليف هذا الشعور بنسميه شعور فطري لا يمكن
+
+128
+00:11:46,040 --> 00:11:52,480
+رفعه شعور فطري يعني كل واحد ممكن يتعبمن أنه أو يجد
+
+129
+00:11:52,480 --> 00:11:58,980
+مشقة من أنه يقوم للصلاة ويتوضى ويصلي لكن هذا
+
+130
+00:11:58,980 --> 00:12:04,480
+الشعور المشقة بسيط ويمكن يتغلب عليه الإنسان وهو
+
+131
+00:12:04,480 --> 00:12:10,990
+شعور فطري لا يمكن يعني إزالته من نفس الإنسانإن نفس
+
+132
+00:12:10,990 --> 00:12:16,990
+الإنسان حب الإنسان الراحة والخلود إلى يعني العدم
+
+133
+00:12:16,990 --> 00:12:24,290
+القيم من النوم وعدم فعل أي أمر يسبب له تعب هذا
+
+134
+00:12:24,290 --> 00:12:29,450
+التعب وإن كان بسيط لكن المشارع يعلم أن المكلف ممكن
+
+135
+00:12:29,450 --> 00:12:33,290
+أن يتغلب على هذا التعب وعلى هذا الشعور الفطري
+
+136
+00:12:33,290 --> 00:12:39,750
+ويحقق العبادةهنا عندنا بالنسبة لأقسام المحكم فيه
+
+137
+00:12:39,750 --> 00:12:44,290
+مش هناخده بالتفصيل لكن هنستعرضه استعراض الحنفية
+
+138
+00:12:44,290 --> 00:12:49,090
+قسم وفعل المكلف من حيث صلته بأحكام الله تعالى
+
+139
+00:12:49,090 --> 00:12:53,990
+وحقوق العباد إلى أربع أقسام حق خالص لله تعالى، حق
+
+140
+00:12:53,990 --> 00:12:58,830
+خالص للعبد، مجتمع فيه الحقان وحق الله غالب، مجتمع
+
+141
+00:12:58,830 --> 00:13:03,800
+فيه الحقان وحق العبد غالبهذه تقسيم الحقوق أو تقسيم
+
+142
+00:13:03,800 --> 00:13:09,460
+الأقصام المحكوم فيها عند الحنافية بس يعني إعرفوهم
+
+143
+00:13:09,460 --> 00:13:14,520
+معرفة بدون تفصيل بدون تعريف لكل حق من الحقوق هذه
+
+144
+00:13:14,520 --> 00:13:20,600
+بدون أن نستعرض أمثلة على هذه الحقوق هذا بس للإعلان
+
+145
+00:13:20,600 --> 00:13:25,000
+فقط الآن
+
+146
+00:13:25,000 --> 00:13:31,560
+سنتحدث عن المحكوم عليه المحكوم عليه الآن هو المكلف
+
+147
+00:13:32,040 --> 00:13:38,220
+ومن هنا بدنا نبدأ طبعا الكلام المهم الذي يتعلق
+
+148
+00:13:38,220 --> 00:13:44,640
+بالمكلف المكلف هو الشخص البلغ العقل الذي تعلق خطاب
+
+149
+00:13:44,640 --> 00:13:51,060
+الشارع بفعله ووجه إليه الأحكام وطالبه بتنفيذها شرط
+
+150
+00:13:51,060 --> 00:13:56,120
+صحة التكليف أو شرط صحة التكليف المكلف طبعا عندي
+
+151
+00:13:56,120 --> 00:14:00,400
+شرطين ومهمين هذول الشرطين الشرط الأول اللي هو
+
+152
+00:14:00,400 --> 00:14:04,320
+العقل والفهموالشرط الثاني اللي هو الأهلية اللي
+
+153
+00:14:04,320 --> 00:14:09,820
+هنتكلم عنها ان شاء الله بتفصيل كبير في الفصل
+
+154
+00:14:09,820 --> 00:14:15,100
+الأخير اللي هو الفصل التالت ان شاء الله تعالى أما
+
+155
+00:14:15,100 --> 00:14:19,040
+بالنسبة ليه الشرط الأول العقل والفهم؟ يعني أن يكون
+
+156
+00:14:19,040 --> 00:14:23,360
+المكلف قادرا على فهم دليل التكليف بأن يفهم الكلام
+
+157
+00:14:23,360 --> 00:14:27,140
+الذي وجهه إليه والمراد بإن الفهم استطاعت فهم
+
+158
+00:14:27,140 --> 00:14:32,600
+المكلف للخطاب وتصوره وليس تصديق بهعشان هيك بنقول
+
+159
+00:14:32,600 --> 00:14:37,080
+الكفار مخاطبون بالإيمان وإن لم يقع منهم تصديق
+
+160
+00:14:37,080 --> 00:14:40,580
+باتفاق العلماء لأن الرسول صلى الله عليه وسلم مبعوث
+
+161
+00:14:40,580 --> 00:14:44,960
+إلى جميع الخلق عشان هيك يقول أحد علماء الأصول وهو
+
+162
+00:14:44,960 --> 00:14:49,300
+الشوكاني لا خلافة في أنهم مخاطبون يعني الكفار بأمر
+
+163
+00:14:49,300 --> 00:14:53,080
+الإيمان لأنه النبي صلى الله عليه وسلم مبعوث إلى
+
+164
+00:14:53,080 --> 00:14:59,720
+الكافة و بالمعاملات أيضا دليل هذا الشرطلزوم المحال
+
+165
+00:14:59,720 --> 00:15:05,480
+عند عدم اعتباره في التكليف كيف؟ لأن طلب حصول الفعل
+
+166
+00:15:05,480 --> 00:15:09,960
+من المكلف يقصد به الامتثال والابتلاء وذلك مستحيل
+
+167
+00:15:09,960 --> 00:15:15,640
+عادة وشرعا ممن لا شعور له ولأنه لو صح ذلك التكليف
+
+168
+00:15:15,640 --> 00:15:20,520
+لكل فترة البهائم التي حالة عدم الفهم بينها وبين
+
+169
+00:15:20,520 --> 00:15:26,630
+الخطاب يعني لابد أن يوجد العقل والفهم حتىيتم تكليف
+
+170
+00:15:26,630 --> 00:15:29,970
+المكلف بترتب على هذا الشرط عدة أمور هذه الأمور
+
+171
+00:15:29,970 --> 00:15:33,650
+اللي هنستعرضها سريعا لها تفصيل في الحديث عن
+
+172
+00:15:33,650 --> 00:15:39,650
+الأهلية عدم تكليف طبعا المجنون والصبي هذا إشي يعني
+
+173
+00:15:39,650 --> 00:15:46,550
+بديهي كيف نكلف من لا عقل له ومن لا إدراك له وهذا
+
+174
+00:15:46,550 --> 00:15:50,890
+هنتكلم ان شاء الله بالتفصيل عنه عدم تكليف النائم
+
+175
+00:15:50,890 --> 00:15:56,300
+والغافل والسكرانبرضه لنا حديث طويل عنهم طبعا عدم
+
+176
+00:15:56,300 --> 00:16:02,480
+تكليفهم بالعبادة لكن النفقة للأقارب والزكاة وجوب
+
+177
+00:16:02,480 --> 00:16:05,940
+الضمان فيما يتلفه الصبي والمجنون ليس من خطاب
+
+178
+00:16:05,940 --> 00:16:09,900
+التكليف إنما من خطاب الوضع اللي هو ربط الأسباب
+
+179
+00:16:09,900 --> 00:16:14,140
+بالمسببات لأن هذه الواجبات لم تتعلق بفعل الصبي
+
+180
+00:16:14,140 --> 00:16:19,000
+والمجنون إنما تعلقت بالمال أو الذمة وهذا برضه
+
+181
+00:16:19,000 --> 00:16:23,670
+هنوضحه أكتر لما نتكلم عنأهلية الصبي وأهلية المجنون
+
+182
+00:16:23,670 --> 00:16:30,810
+ليش؟ لأنهم صالحان بما ثبت لهم من إنسانية تهيئتهم
+
+183
+00:16:30,810 --> 00:16:36,310
+لفهم الخطاب عند حدوث البلوغ والإفاقة ومتول الأداء
+
+184
+00:16:36,310 --> 00:16:42,550
+هو الولي بعد بلوغ الصبي وإفاقة المجنون كمان مرة
+
+185
+00:16:42,550 --> 00:16:48,690
+بقول إن هذا الكلام هنقوم بتفصيلهإن شاء الله عندها
+
+186
+00:16:48,690 --> 00:16:52,730
+في الفصل الثالث وهو الأخير معناه بيكون إن شاء الله
+
+187
+00:16:52,730 --> 00:16:57,170
+الشرط التاني الأهلية طبعاً أن يكون المكلف أهلا
+
+188
+00:16:57,170 --> 00:17:01,330
+للتكليف وأهلية المكلف لا تتحقق إلا بالعقل والفهم
+
+189
+00:17:01,330 --> 00:17:07,550
+لأن العقل شرط للتكليف برضه موضوع الأهلية إن شاء
+
+190
+00:17:07,550 --> 00:17:13,740
+الله نفصل الحديث فيه بتعريف الأهليةوسنتكلم عن
+
+191
+00:17:13,740 --> 00:17:19,060
+عوارض الأهلية السماوية والمكتسبة بإذن الله تعالى
+
+192
+00:17:19,060 --> 00:17:25,140
+كما قلنا في الفصل الثالث بإذن الله تعالى نكتفي
+
+193
+00:17:25,140 --> 00:17:29,740
+بهذا القدر في هذه المحاضرة على أن نلقاكم في
+
+194
+00:17:29,740 --> 00:17:34,180
+المحاضرة القادمة السلام عليكم ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/zJ_ZuDD_IQs_raw.srt b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/zJ_ZuDD_IQs_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..fc6cafd3897da70cac66509b8df0e921653ebde2
--- /dev/null
+++ b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/zJ_ZuDD_IQs_raw.srt
@@ -0,0 +1,596 @@
+1
+00:00:11,240 --> 00:00:13,920
+أعوذ بالله من الشيطان الرجيم بسم الله الرحمن
+
+2
+00:00:13,920 --> 00:00:17,560
+الرحيم الحمد لله الواحد الأحد الفرد الصمد الذي لم
+
+3
+00:00:17,560 --> 00:00:21,480
+يلد ولم يولد ولم يكن له كفوا أحد وصلاة والسلام على
+
+4
+00:00:21,480 --> 00:00:27,160
+خير الأنام محمد وعلى آله وصحبه الكرام وبعد بداية
+
+5
+00:00:27,160 --> 00:00:31,040
+أخوات الحبيبات وبنات الطيبات أحييكم بتحية الإسلام
+
+6
+00:00:31,040 --> 00:00:35,150
+العظيم السلام عليكم ورحمة الله وبركاتهكنا قد
+
+7
+00:00:35,150 --> 00:00:40,330
+انتهينا في المحاضرة السابقة من الحديث عن أقسام
+
+8
+00:00:40,330 --> 00:00:46,770
+الأهلية وقولنا أن أقسام الأهلية قسمين الأول أهلية
+
+9
+00:00:46,770 --> 00:00:50,990
+الوجوب والثاني أهلية الأداء طبعا أهلية الوجوب
+
+10
+00:00:50,990 --> 00:00:56,590
+أهلية ناقصة وكاملة وأهلية الأداء منعدمة وناقصة
+
+11
+00:00:56,590 --> 00:01:01,540
+وكاملة وفصلنا الحديث عن ذلكاليوم ان شاء الله
+
+12
+00:01:01,540 --> 00:01:08,880
+هنتكلم عن عوارض الأهلية بالنسبة لمعنى العوارض في
+
+13
+00:01:08,880 --> 00:01:14,940
+اللغة العارض هو المانع أو الحائل ومعنى اعترض الشيء
+
+14
+00:01:14,940 --> 00:01:20,760
+صار عارضا واعترض دونه حالة واعترض له أي منعه
+
+15
+00:01:20,760 --> 00:01:26,300
+والعارض أيضا ما اعترض في الأفق فسده من سحاب او نحل
+
+16
+00:01:26,300 --> 00:01:31,760
+او جراد او نحو ذلك ومنه قول اللهتعالى عن قوم عاد
+
+17
+00:01:31,760 --> 00:01:37,620
+هذا عارض ممطرنا والـ A فتعرض في الشيء أي تحول
+
+18
+00:01:37,620 --> 00:01:44,800
+وتمنع ويسمى السحاب بالعارض لمنعه أثر الشمس وشعاعها
+
+19
+00:01:44,800 --> 00:01:51,320
+معنى العوارض في الإصطلاح هي أحوال تطرق على الإنسان
+
+20
+00:01:51,320 --> 00:01:57,760
+المكلف في أهليته دون أن تكون ملازمة له هذه الأمور
+
+21
+00:01:57,760 --> 00:02:03,640
+التي لها تأثيرفي تغيير الأحكام عوارض لمنعها
+
+22
+00:02:03,640 --> 00:02:09,660
+الأحكام التي تتعلق بأهلية الوجوب وأهلية الأداء عن
+
+23
+00:02:09,660 --> 00:02:15,760
+الثبوت بالنسبة لتأثير العوارض طبعا ما سبق أن
+
+24
+00:02:15,760 --> 00:02:20,380
+تحدثنا عنه في الأهلية هو بناء على الحالة الطبيعية
+
+25
+00:02:20,380 --> 00:02:24,500
+التي يكون فيها الشخص سليما غير أنه قد يترأ على
+
+26
+00:02:24,500 --> 00:02:28,900
+الأشخاص عوارض وهذه العوارضذات تأثير على أهلية
+
+27
+00:02:28,900 --> 00:02:33,520
+الإنسان فإما أن تكون مزيلة لأهلية الوجوب كما في
+
+28
+00:02:33,520 --> 00:02:39,300
+الموت فالموت عاجزة منافق لأهلية أحكام الدنيا من كل
+
+29
+00:02:39,300 --> 00:02:44,700
+ما فيه تكليف وهذه العوارض تزيل أحيانا أهلية الأداء
+
+30
+00:02:44,700 --> 00:02:48,760
+كحالتين النوم والاغماء وأحيانا أخرى لا تزيل هذه
+
+31
+00:02:48,760 --> 00:02:53,140
+العوارض لأهلية الوجوب ولا أهلية الأداء لكنها تغير
+
+32
+00:02:53,140 --> 00:02:58,700
+بعض الأحكام مع باقائهممع بقاء أهلية الوجوه و أهلية
+
+33
+00:02:58,700 --> 00:03:03,240
+الأداء كما في حالتي السفر والجهل في دار الحرب كل
+
+34
+00:03:03,240 --> 00:03:09,400
+هذا طبعا هيجي الحديث عن التفصيل فيه بالنسبة لعوارض
+
+35
+00:03:09,400 --> 00:03:15,600
+علماء الوصول يقسم العوارض إلى قسمين رئيسيين هما
+
+36
+00:03:15,600 --> 00:03:20,260
+العوارض السماوية و العوارض المكتسبة خلينا نتحدث
+
+37
+00:03:20,260 --> 00:03:27,830
+اليوم عن العوارض السماويةو قبل أن نبدأ بهذه
+
+38
+00:03:27,830 --> 00:03:34,710
+العوارض خلّينا نقف على حقيقة العوارض السماوية طبعا
+
+39
+00:03:34,710 --> 00:03:39,530
+العوارض السماوية ما ثبت من قبل صاحب الشرع بدون
+
+40
+00:03:39,530 --> 00:03:46,170
+اختيار للشخص فيه أي الإنسان ليس له كسب ولا اختيار
+
+41
+00:03:46,170 --> 00:03:52,730
+في هذا الأمر طبعا هذه العوارض لا دخلة للإنسان فيها
+
+42
+00:03:53,300 --> 00:03:57,020
+ونصبت إلى السماء لنزولها من السماء وهي خارجة عن
+
+43
+00:03:57,020 --> 00:04:00,840
+قدرة الإنسان وقدم العلماء الحديث عن العوارض
+
+44
+00:04:00,840 --> 00:04:05,280
+السماوية على العوارض المكتسبة ليش؟ لأنها أظهر في
+
+45
+00:04:05,280 --> 00:04:09,920
+العارضية لخروجها عن اختيار الإنسان وأشد تأثيرا في
+
+46
+00:04:09,920 --> 00:04:16,880
+الأحكام المكتسبة طبعا بالنسبة للعوارض السماوية
+
+47
+00:04:16,880 --> 00:04:24,220
+قسموها العلماء إلى 11 قسمالصغر والجنون والعته
+
+48
+00:04:24,220 --> 00:04:29,120
+والنسيان والنوم والاغماء والرق اللي مش هنتحدث عنه
+
+49
+00:04:29,120 --> 00:04:35,560
+والمرض والحيد والنفس والموت طبعا قبل بنبدأ بالحديث
+
+50
+00:04:35,560 --> 00:04:40,560
+عن هذه العوارض كل عارض من هذه العوارض بدك تعمل
+
+51
+00:04:40,560 --> 00:04:46,620
+حسابك أنك بدك تعمليله صفحة لوحدها و تبدأ بتلخيص كل
+
+52
+00:04:46,620 --> 00:04:52,120
+عارض لوحده بإيدك بقلمك تمسك القلم و بتبدأالصغر
+
+53
+00:04:52,120 --> 00:04:56,120
+مثلا تعريفه و الأحكام اللي متعلقة فيه تأثيره على
+
+54
+00:04:56,120 --> 00:05:01,440
+ال .. ال .. طبعا الأحكام التكلفية أو عقود البيع
+
+55
+00:05:01,440 --> 00:05:06,420
+مدى تأثيره على أهلية الوجوب أهلية الأداء على حسب
+
+56
+00:05:06,420 --> 00:05:11,400
+يعني ما احنا هنشرح عنه معناه ذلك انك انت الآن
+
+57
+00:05:11,400 --> 00:05:16,100
+بالنسبة للأعراض العوارض السماوية العشر عوارض هدول
+
+58
+00:05:16,100 --> 00:05:19,220
+لإنه احنا هنستثنى الرق لإنه مافيش إله داعي لحديث
+
+59
+00:05:19,220 --> 00:05:23,950
+عنه اليوم لإنه مش موجودهيكون عندك عشر ورقات من
+
+60
+00:05:23,950 --> 00:05:27,570
+دفتر أبو أربعين، أدول العشر ورقات فيهم تلخيص
+
+61
+00:05:27,570 --> 00:05:32,930
+للعوارض السماوية زي ما هنحكي كل عارض إيش هيتعلق
+
+62
+00:05:32,930 --> 00:05:37,630
+فيه و هيك بتكون سهلت عليك و يسرطلك الأمر في
+
+63
+00:05:37,630 --> 00:05:43,930
+الدراسة، للاختبار، إن هي إيه بإذن الله تعالى نبدأ
+
+64
+00:05:43,930 --> 00:05:48,370
+الحديث عن العارض الأول وهو الصغر، زي ما اتفقنا
+
+65
+00:05:48,370 --> 00:05:52,620
+بدنا نبدأ بتعريف الصغرطبعا زي ما انتوا شايفين
+
+66
+00:05:52,620 --> 00:05:58,160
+التعريف لازم يكون صغير مش كبير هذا بيسموه العلماء
+
+67
+00:05:58,160 --> 00:06:05,480
+تعريف بالرسم أو التعريف بالأثر بالنسبالي الصغر هو
+
+68
+00:06:05,480 --> 00:06:10,760
+المرحلة اللتي تسبق طور البلوغ سواء كان هذا البلوغ
+
+69
+00:06:10,760 --> 00:06:14,640
+طبيعيا عن طريق العلامات الطبيعية كخروج المنو و
+
+70
+00:06:14,640 --> 00:06:22,050
+انبات الشعر أو الحمل أو نزول الدمالحيد او تقديريا
+
+71
+00:06:22,050 --> 00:06:28,410
+اي عن طريق تقدير العمر كتقديره بخمسة عشرة عامة اذا
+
+72
+00:06:28,410 --> 00:06:33,150
+البلوغ هو عبارة عن مرحلة تسبق طور البلوغ الصغر
+
+73
+00:06:33,150 --> 00:06:38,770
+اسفة الصغر هو عبارة عن مرحلة تسبق طور البلوغ طبعا
+
+74
+00:06:38,770 --> 00:06:43,170
+ايش البلوغ؟ البلوغ ده ممكن يكون طبيعي بظهور
+
+75
+00:06:43,170 --> 00:06:48,830
+العلامات الطبيعية على ال .. ال .. الولد او البنت
+
+76
+00:06:49,030 --> 00:06:55,330
+حسب يعني المعداد أو تقديريا يعني مافيش عندي علامات
+
+77
+00:06:55,330 --> 00:07:00,530
+طبيعية بروح بعمل تقدير عن طريق تقدير العمر و أحدده
+
+78
+00:07:00,530 --> 00:07:08,330
+العلماء بخمسة عشرة عامة نيجي لمراحل الصغر و هان
+
+79
+00:07:08,330 --> 00:07:12,930
+احنا الحديث هيكون المرحلة الأولى ما قبل التمييز و
+
+80
+00:07:12,930 --> 00:07:16,630
+المرحلة التانية اللي هي مرحلة التمييز طبعا كل
+
+81
+00:07:16,630 --> 00:07:22,710
+مرحلة من هذه المراحللها أحكام تتعلق بها مرحلة ما
+
+82
+00:07:22,710 --> 00:07:28,790
+قبل التمييز تبدأ هذه المرحلة من حين مولد الصبي إلى
+
+83
+00:07:28,790 --> 00:07:34,410
+أن يقارب سن السابعة يعني من حين الولادة لسن سبعة
+
+84
+00:07:34,410 --> 00:07:37,930
+سنين في هذه المرحلة هذه الأحكام اللي بتتعلق بيها
+
+85
+00:07:37,930 --> 00:07:42,850
+تسقط عنه العبادات لا يصح تكليفه لفقده العقل
+
+86
+00:07:42,850 --> 00:07:48,460
+والتمييز معهلكن يؤخذ بضمان الأفعال في المطلفات
+
+87
+00:07:48,460 --> 00:07:53,460
+وحكينا مثال يمكن أنتم متذكرينه حكيناه مرة في
+
+88
+00:07:53,460 --> 00:08:00,320
+المحادرات السابقة أن جدتنا زمان إيش كانت يقول أو
+
+89
+00:08:00,320 --> 00:08:04,520
+جدادنا الكبار إيش كانوا يحكوا لصغير ورا إيش ورا
+
+90
+00:08:04,520 --> 00:08:09,940
+كبير هي معناة ضمان المطلفات مش معقول الصبي يطلف
+
+91
+00:08:09,940 --> 00:08:17,730
+مال الغيروالتلف هذا يكون مسامح فيه، لأ هذا لابد أن
+
+92
+00:08:17,730 --> 00:08:21,350
+يأخذ بالضمان حتى يحافظ على ممتلكات الآخرين يعني
+
+93
+00:08:21,350 --> 00:08:25,210
+حكمة المشرع من ضمان المتلفات اللي هي إيه المحافظة
+
+94
+00:08:25,210 --> 00:08:30,040
+على ضمان الآخرين وإذا بتتذكروا لما حكيتلكمالولد
+
+95
+00:08:30,040 --> 00:08:35,460
+اللي أمه ماخدها معاها على محل .. و هذا المحل بيبيع
+
+96
+00:08:35,460 --> 00:08:39,940
+فناجين و كبيات و إزاز و كذا و راح كصرف من الفناجين
+
+97
+00:08:39,940 --> 00:08:43,980
+هذه بنفع أمه تقول لصاحب المحل أسفة و تمشي و لا
+
+98
+00:08:43,980 --> 00:08:49,500
+بتدفع بدل المطلف هذا؟ هيدفعها بدل المطلف هذا المرة
+
+99
+00:08:49,500 --> 00:08:54,300
+الجاية مش هتاخد ابنها معاها أو ه .. هي تمسكه في
+
+100
+00:08:54,300 --> 00:08:58,490
+إيدها و مش هتسيبهيتلف أموال الآخرين وان هنا تظهر
+
+101
+00:08:58,490 --> 00:09:02,550
+حكمة المشرع في المحافظة على الأموال الآخرين بهذا
+
+102
+00:09:02,550 --> 00:09:07,050
+الضمان إذن مرحلة ما قبل التمييز تبدأ من حين
+
+103
+00:09:07,050 --> 00:09:12,350
+الولادة إلى سن السابعة هذه المرحلة تسقط عنه
+
+104
+00:09:12,350 --> 00:09:17,150
+العبادات ولا يصح تكلفه لفقده العقل والتمييز معا
+
+105
+00:09:17,150 --> 00:09:22,950
+ولكن يؤخذ بضمان الأفعال في المطلفات فزي ما قلنا لو
+
+106
+00:09:22,950 --> 00:09:28,100
+أطلف مال غيره ضمنأما بالنسبة لأقواله فلا تعتبر
+
+107
+00:09:28,100 --> 00:09:33,400
+شرعا، يعني لو باع، لو رهن، لو أجر، كل هذه أقواله
+
+108
+00:09:33,400 --> 00:09:37,860
+باطلة وغير معتبرة، لعدم تعقل المعاني، وعلى هذا فلا
+
+109
+00:09:37,860 --> 00:09:43,220
+تصح لا عقوده ولا إكراراته، وحتى إن أجازها الولي في
+
+110
+00:09:43,220 --> 00:09:48,840
+مرحلة ما قبل التمييز، معناه هذاإنه الصغير في هذه
+
+111
+00:09:48,840 --> 00:09:53,580
+الحالة كالمجنون بل هو أدنى منه لأن المجنون قد يميز
+
+112
+00:09:53,580 --> 00:09:58,980
+وإن فقد العقل أما الصغير ففاقد للعقل والتمييز معاه
+
+113
+00:09:58,980 --> 00:10:05,020
+نيجي لمرحلة ثانية من مراحل التمييز تبدأ طبعا هان
+
+114
+00:10:05,020 --> 00:10:09,900
+من حيث انتهينا من المرحلة الأولى وهي سن السابعة
+
+115
+00:10:09,900 --> 00:10:15,800
+وتنتهي بالبلوغفي هذه المرحلة يرقى الطفل بيكبر تبدو
+
+116
+00:10:15,800 --> 00:10:20,980
+عليه آثار العقل أصبح صاحب أهلية وكان ينبغي أن يثبت
+
+117
+00:10:20,980 --> 00:10:26,500
+في حقه وجوب الأداء بحسب وجود الأهلية لكن الشارع
+
+118
+00:10:26,500 --> 00:10:31,340
+اعتبره اعتبر صغره عذرا لأن الأهلية التي نالها
+
+119
+00:10:31,340 --> 00:10:37,040
+أهلية قاسرة لقصور عقله بسبب بقاء صغره وعدم بلوغ
+
+120
+00:10:37,040 --> 00:10:41,840
+عقله غير الاعتدال هنا الأحكام اللي بتتعلق فيهتسقط
+
+121
+00:10:41,840 --> 00:10:45,500
+عن المميز ما يحتمل السقوط من حقوق الله تعالى
+
+122
+00:10:45,500 --> 00:10:50,260
+كالصوم والصلاة وسائر الإعبادات فإنها محتملة للسقوط
+
+123
+00:10:50,260 --> 00:10:54,980
+عند وجود الأعذار أصلا يعني ولا يسقط عنه ما لا
+
+124
+00:10:54,980 --> 00:11:00,620
+يحتمل السقوط كعقيدة الإيمان لأنه فرض دائم لا يقبل
+
+125
+00:11:00,620 --> 00:11:05,680
+السقوط لأن الله تعالى إله دائم منزه عن الزوال
+
+126
+00:11:05,680 --> 00:11:12,330
+والتغيير فأصبح وجوب التوحيد دائما بدوامالألوهية و
+
+127
+00:11:12,330 --> 00:11:17,010
+لو سألناكوا ما سبب اختيار الفقهاء لأن تكون سن
+
+128
+00:11:17,010 --> 00:11:20,930
+السابعة بداية مرحلة التمييز عند الصغير على طول
+
+129
+00:11:20,930 --> 00:11:25,310
+الجواب بيكون اه صح يا أسماء bravo عليك يا صفية
+
+130
+00:11:25,310 --> 00:11:30,580
+دايما بتسبق ايه؟في الإجابة وهو قول النبي صلى الله
+
+131
+00:11:30,580 --> 00:11:34,460
+عليه وسلم قروصوا بيانكم بالصلاة لسبع سنين واضربوهم
+
+132
+00:11:34,460 --> 00:11:38,720
+عليها لعشر سنين وفرقوا بينهم في المضاجع فكان هذا
+
+133
+00:11:38,720 --> 00:11:44,520
+دليل على اختيار الفقهة أو الوقوف على سن السابع وهي
+
+134
+00:11:44,520 --> 00:11:49,320
+بداية لمرحلة التمييز عند الصغير طبعا هنا لا يخفى
+
+135
+00:11:49,320 --> 00:11:51,700
+عليكم أن الأمر بالصلاة في حديث الرسول صلى الله
+
+136
+00:11:51,700 --> 00:11:56,540
+عليه وسلمفي هذه السنة من العمر بمثابة الأمر
+
+137
+00:11:56,540 --> 00:12:01,180
+بالتمرين والتدريب للصبي لأجل أن يعتاد أداء الصلاة
+
+138
+00:12:01,180 --> 00:12:07,700
+عند غلوغه واكتمال عقله وسمي الصبي مميزا لأنه أصبح
+
+139
+00:12:07,700 --> 00:12:12,160
+الأن يميز بين الأقوال والأفعال بيفرق عن المرحلة
+
+140
+00:12:12,160 --> 00:12:16,060
+قبل التمييز قبل السن السابع ماكانش يفهم ولا كان
+
+141
+00:12:16,060 --> 00:12:21,620
+يميز لكن هنايعني بدأ عقله ينمو و يكبر فأصبح يميز
+
+142
+00:12:21,620 --> 00:12:26,820
+بين الأقوال و الأفعال و بين الخير و الشر و بين
+
+143
+00:12:26,820 --> 00:12:33,640
+الجيد و الراضي هذا الحديث كان عن مرح عن الصغر إذا
+
+144
+00:12:33,640 --> 00:12:42,310
+لو سألتك سؤالعرفي الصغر واتحدثي عن المراحل التي
+
+145
+00:12:42,310 --> 00:12:48,150
+يمروا بها الصغير وما يتعلق بها من أحكام إجابتك
+
+146
+00:12:48,150 --> 00:12:52,730
+بتكون الكلام اللي حكيناه سابقا أكتفي بهذا القدر في
+
+147
+00:12:52,730 --> 00:12:57,090
+هذه المحاضرة على أن أستكمل باقي العوارض السماوية
+
+148
+00:12:57,090 --> 00:13:00,950
+في المحاضرة القادمة بإذن الله و السلام عليكم و
+
+149
+00:13:00,950 --> 00:13:02,570
+رحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/IFIlLjkAPfo_raw.json b/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/IFIlLjkAPfo_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..4f9d807bf00bc036ba35e4a983ec1be2e48254eb
--- /dev/null
+++ b/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/IFIlLjkAPfo_raw.json
@@ -0,0 +1 @@
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يكون", "probability": 0.96044921875}, {"start": 4579.7, "end": 4580.06, "word": " نظيف", "probability": 0.8219401041666666}, {"start": 4580.06, "end": 4580.22, "word": " في", "probability": 0.6689453125}, {"start": 4580.22, "end": 4580.6, "word": " النار", "probability": 0.8504231770833334}, {"start": 4580.6, "end": 4581.54, "word": " اول", "probability": 0.7120361328125}, {"start": 4581.54, "end": 4581.88, "word": " حاجة", "probability": 0.9710286458333334}, {"start": 4581.88, "end": 4582.1, "word": " بدي", "probability": 0.6456298828125}, {"start": 4582.1, "end": 4582.68, "word": " اقعده", "probability": 0.8668212890625}, {"start": 4582.68, "end": 4584.12, "word": " مايكونش", "probability": 0.76751708984375}, {"start": 4584.12, "end": 4584.46, "word": " نايم", "probability": 0.9334309895833334}, {"start": 4584.46, "end": 4584.88, "word": " لانه", "probability": 0.8141276041666666}, {"start": 4584.88, "end": 4585.0, "word": " دي", "probability": 0.33905029296875}, {"start": 4585.0, "end": 4585.24, "word": " بنام", "probability": 0.479248046875}, {"start": 4585.24, "end": 4585.88, "word": " بنخنج", "probability": 0.7525634765625}, {"start": 4585.88, "end": 4587.32, "word": " بدي", "probability": 0.846923828125}, {"start": 4587.32, "end": 4587.9, "word": " اعمله", "probability": 0.9518229166666666}, {"start": 4587.9, "end": 4589.84, "word": " كمدات", "probability": 0.895751953125}, {"start": 4589.84, "end": 4590.58, "word": " تلج", "probability": 0.9763997395833334}, {"start": 4590.58, "end": 4590.92, "word": " على", "probability": 0.70068359375}, {"start": 4590.92, "end": 4591.46, "word": " راسه", "probability": 0.9544270833333334}, {"start": 4591.46, "end": 4593.04, "word": " وين", "probability": 0.70947265625}, {"start": 4593.04, "end": 4593.48, "word": " بتحط", "probability": 0.8878580729166666}, {"start": 4593.48, "end": 4594.0, "word": " كمدات", "probability": 0.9434814453125}, {"start": 4594.0, "end": 4594.4, "word": " التلج", "probability": 0.9837239583333334}, {"start": 4594.4, "end": 4594.56, "word": " على", "probability": 0.84228515625}, {"start": 4594.56, "end": 4594.98, "word": " الراس", "probability": 0.8271484375}, {"start": 4594.98, "end": 4596.34, "word": " عكس", "probability": 0.9402669270833334}, {"start": 4596.34, "end": 4596.82, "word": " الجهة", "probability": 0.96484375}, {"start": 4596.82, "end": 4598.34, "word": " المصابة", "probability": 0.9041748046875}, {"start": 4598.34, "end": 4598.74, "word": " اذا", "probability": 0.778076171875}, {"start": 4598.74, "end": 4599.26, "word": " انشلت", "probability": 0.936767578125}, {"start": 4599.26, "end": 4599.76, "word": " اليمين", "probability": 0.8099772135416666}, {"start": 4599.76, "end": 4600.7, "word": " بحط", "probability": 0.9425455729166666}, {"start": 4600.7, "end": 4601.06, "word": " التلج", "probability": 0.9856770833333334}, {"start": 4601.06, "end": 4601.2, "word": " على", "probability": 0.8212890625}, {"start": 4601.2, "end": 4601.44, "word": " الراس", "probability": 0.8583984375}, {"start": 4601.44, "end": 4602.52, "word": " وين؟", "probability": 0.82421875}, {"start": 4602.52, "end": 4603.54, "word": " في", "probability": 0.8515625}, {"start": 4603.54, "end": 4604.02, "word": " الجهة", "probability": 0.9850260416666666}, {"start": 4604.02, "end": 4604.66, "word": " الشمال", "probability": 0.8933919270833334}], "temperature": 1.0}, {"id": 163, "seek": 463085, "start": 4605.17, "end": 4630.85, "text": "و بدي اهتم في التنفس و اذا بدي اخليه على جنبه بدي اخليه على جنبه السليم لإنه ممكن يصير افرازات منه انه صار افرازات منه المنطقة مشلودة بيشرج فيها و بيسكر نفسه و مش داري لكن اذا كان على الشجة السليمة ممكن يتعامل معاها يتفها يتخلص منها بطريقة و هو صاحي و بنوديه على اقرب مركز طبي مع", "tokens": [2407, 4724, 16254, 1975, 3224, 39237, 8978, 16712, 1863, 36178, 4032, 1975, 15730, 4724, 16254, 1975, 9778, 20292, 3224, 15844, 10874, 1863, 3555, 3224, 4724, 16254, 1975, 9778, 20292, 3224, 15844, 10874, 1863, 3555, 3224, 21136, 20292, 2304, 5296, 28814, 1863, 3224, 3714, 43020, 7251, 9381, 13546, 1975, 5172, 2288, 31377, 9307, 9154, 3224, 16472, 3224, 20328, 9640, 1975, 5172, 2288, 31377, 9307, 9154, 3224, 9673, 1863, 9566, 28671, 37893, 1211, 23328, 3660, 4724, 1829, 46309, 7435, 8978, 11296, 4032, 4724, 1829, 3794, 37983, 8717, 36178, 3224, 4032, 37893, 11778, 9640, 1829, 44381, 1975, 15730, 25961, 15844, 25124, 7435, 3660, 21136, 20292, 46007, 3714, 43020, 7251, 2655, 3615, 10943, 1211, 20449, 995, 11296, 7251, 2655, 5172, 11296, 7251, 2655, 9778, 1211, 9381, 9154, 11296, 4724, 9566, 16572, 28671, 4032, 31439, 20328, 39319, 1829, 4032, 44945, 23328, 1829, 3224, 15844, 1975, 4587, 25513, 3714, 31747, 11622, 23032, 21292, 20449], "avg_logprob": -0.15908137583892618, "compression_ratio": 2.141025641025641, "no_speech_prob": 7.152557373046875e-07, "words": [{"start": 4605.17, "end": 4605.43, "word": "و", "probability": 0.51220703125}, {"start": 4605.43, "end": 4605.81, "word": " بدي", "probability": 0.6175537109375}, {"start": 4605.81, "end": 4606.29, "word": " اهتم", "probability": 0.9150390625}, {"start": 4606.29, "end": 4606.45, "word": " في", "probability": 0.6376953125}, {"start": 4606.45, "end": 4607.01, "word": " التنفس", "probability": 0.97802734375}, {"start": 4607.01, "end": 4607.37, "word": " و", "probability": 0.93994140625}, {"start": 4607.37, "end": 4607.51, "word": " اذا", "probability": 0.88037109375}, {"start": 4607.51, "end": 4607.79, "word": " بدي", "probability": 0.760498046875}, {"start": 4607.79, "end": 4608.37, "word": " اخليه", "probability": 0.828125}, {"start": 4608.37, "end": 4608.53, "word": " على", "probability": 0.85498046875}, {"start": 4608.53, "end": 4608.93, "word": " جنبه", "probability": 0.9805908203125}, {"start": 4608.93, "end": 4609.13, "word": " بدي", "probability": 0.774169921875}, {"start": 4609.13, "end": 4609.49, "word": " اخليه", "probability": 0.9393310546875}, {"start": 4609.49, "end": 4609.65, "word": " على", "probability": 0.94384765625}, {"start": 4609.65, "end": 4610.07, "word": " جنبه", "probability": 0.9879150390625}, {"start": 4610.07, "end": 4610.83, "word": " السليم", "probability": 0.95556640625}, {"start": 4610.83, "end": 4611.53, "word": " لإنه", "probability": 0.79949951171875}, {"start": 4611.53, "end": 4611.81, "word": " ممكن", "probability": 0.983642578125}, {"start": 4611.81, "end": 4612.17, "word": " يصير", "probability": 0.8603515625}, {"start": 4612.17, "end": 4612.73, "word": " افرازات", "probability": 0.84736328125}, {"start": 4612.73, "end": 4613.13, "word": " منه", "probability": 0.99365234375}, {"start": 4613.13, "end": 4614.25, "word": " انه", "probability": 0.51611328125}, {"start": 4614.25, "end": 4614.47, "word": " صار", "probability": 0.895751953125}, {"start": 4614.47, "end": 4615.03, "word": " افرازات", "probability": 0.98056640625}, {"start": 4615.03, "end": 4615.37, "word": " منه", "probability": 0.9931640625}, {"start": 4615.37, "end": 4615.95, "word": " المنطقة", "probability": 0.9208984375}, {"start": 4615.95, "end": 4616.65, "word": " مشلودة", "probability": 0.93701171875}, {"start": 4616.65, "end": 4617.65, "word": " بيشرج", "probability": 0.6856689453125}, {"start": 4617.65, "end": 4617.89, "word": " فيها", "probability": 0.98779296875}, {"start": 4617.89, "end": 4617.99, "word": " و", "probability": 0.86865234375}, {"start": 4617.99, "end": 4618.41, "word": " بيسكر", "probability": 0.7032470703125}, {"start": 4618.41, "end": 4618.79, "word": " نفسه", "probability": 0.97119140625}, {"start": 4618.79, "end": 4618.89, "word": " و", "probability": 0.9423828125}, {"start": 4618.89, "end": 4618.99, "word": " مش", "probability": 0.60693359375}, {"start": 4618.99, "end": 4619.25, "word": " داري", "probability": 0.7627766927083334}, {"start": 4619.25, "end": 4619.47, "word": " لكن", "probability": 0.8857421875}, {"start": 4619.47, "end": 4619.73, "word": " اذا", "probability": 0.871337890625}, {"start": 4619.73, "end": 4619.97, "word": " كان", "probability": 0.99755859375}, {"start": 4619.97, "end": 4620.11, "word": " على", "probability": 0.449462890625}, {"start": 4620.11, "end": 4620.45, "word": " الشجة", "probability": 0.8450520833333334}, {"start": 4620.45, "end": 4620.95, "word": " السليمة", "probability": 0.9541015625}, {"start": 4620.95, "end": 4621.83, "word": " ممكن", "probability": 0.989013671875}, {"start": 4621.83, "end": 4622.35, "word": " يتعامل", "probability": 0.992578125}, {"start": 4622.35, "end": 4622.69, "word": " معاها", "probability": 0.7626139322916666}, {"start": 4622.69, "end": 4623.31, "word": " يتفها", "probability": 0.864501953125}, {"start": 4623.31, "end": 4624.51, "word": " يتخلص", "probability": 0.9525390625}, {"start": 4624.51, "end": 4624.91, "word": " منها", "probability": 0.9951171875}, {"start": 4624.91, "end": 4625.61, "word": " بطريقة", "probability": 0.912109375}, {"start": 4625.61, "end": 4625.91, "word": " و", "probability": 0.369384765625}, {"start": 4625.91, "end": 4626.11, "word": " هو", "probability": 0.97314453125}, {"start": 4626.11, "end": 4627.51, "word": " صاحي", "probability": 0.9072265625}, {"start": 4627.51, "end": 4628.15, "word": " و", "probability": 0.92724609375}, {"start": 4628.15, "end": 4628.79, "word": " بنوديه", "probability": 0.799560546875}, {"start": 4628.79, "end": 4628.99, "word": " على", "probability": 0.92626953125}, {"start": 4628.99, "end": 4629.41, "word": " اقرب", "probability": 0.8387044270833334}, {"start": 4629.41, "end": 4629.83, "word": " مركز", "probability": 0.9519856770833334}, {"start": 4629.83, "end": 4630.21, "word": " طبي", "probability": 0.91845703125}, {"start": 4630.21, "end": 4630.85, "word": " مع", "probability": 0.99072265625}], "temperature": 1.0}, {"id": 164, "seek": 463657, "start": 4631.43, "end": 4636.57, "text": "العناية الطبية بمعنى مع مسعف أو مع إسعاف", "tokens": [6027, 3615, 8315, 10632, 41950, 3555, 10632, 4724, 2304, 3615, 1863, 7578, 20449, 47524, 3615, 5172, 34051, 20449, 11933, 3794, 3615, 31845], "avg_logprob": -0.1439368232436802, "compression_ratio": 1.1774193548387097, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 4631.43, "end": 4632.25, "word": "العناية", "probability": 0.9637451171875}, {"start": 4632.25, "end": 4633.57, "word": " الطبية", "probability": 0.9567057291666666}, {"start": 4633.57, "end": 4634.11, "word": " بمعنى", "probability": 0.97353515625}, {"start": 4634.11, "end": 4634.35, "word": " مع", "probability": 0.9296875}, {"start": 4634.35, "end": 4635.03, "word": " مسعف", "probability": 0.8546549479166666}, {"start": 4635.03, "end": 4635.31, "word": " أو", "probability": 0.442626953125}, {"start": 4635.31, "end": 4635.69, "word": " مع", "probability": 0.92919921875}, {"start": 4635.69, "end": 4636.57, "word": " إسعاف", "probability": 0.78564453125}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 4637.2105, "duration_after_vad": 4385.723124999984}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/MZMxT0v6xi8_postprocess.srt b/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/MZMxT0v6xi8_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..09325c01ae10c2818ed0c627cc6af40ae0a71fcf
--- /dev/null
+++ b/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/MZMxT0v6xi8_postprocess.srt
@@ -0,0 +1,2416 @@
+1
+00:00:18,880 --> 00:00:22,480
+قدامنا صورة ان شاء الله بتورينا إيه أنواع الكثور
+
+2
+00:00:22,480 --> 00:00:29,180
+هذه الكثور عبارة عن كثور بسيطة في شكل العظم كثور
+
+3
+00:00:29,180 --> 00:00:33,760
+بسيطة في إيش؟ في شكل العظم بمعنى إيش؟ إيش معناة
+
+4
+00:00:33,760 --> 00:00:40,520
+كسر بسيط في شكل العظم؟ العظم بس شجفتين لحظة العظم
+
+5
+00:00:40,520 --> 00:00:47,280
+بس شجفتين فسمناها كسر بسيطلكن لما تكون العظم اكتر
+
+6
+00:00:47,280 --> 00:00:54,700
+من كسر او اكتر من شجفتين بيسموه ايش متفتت كمان في
+
+7
+00:00:54,700 --> 00:01:02,960
+عندنا الكسر البسيط قولنا اللي هو بيكون فش في الجلد
+
+8
+00:01:02,960 --> 00:01:08,350
+جرحلما يكون فيه جرح في الجلب مع الكسر بنسميه كسر
+
+9
+00:01:08,350 --> 00:01:14,590
+مش بسيط لكن لما يكون بس كسر و جرح مع ال .. ال ..
+
+10
+00:01:14,590 --> 00:01:18,750
+هذا بنسميه .. فيه جرح و فيه كسر بنسميه كسر .. كسر
+
+11
+00:01:18,750 --> 00:01:24,630
+إيش مفتوح هاي في عندك كسر هنا أكتر من كسر شايفات
+
+12
+00:01:24,630 --> 00:01:29,950
+هذا؟ إيش شايفات هنا؟ ملاحظات هذه عظمه و هذه عظمه و
+
+13
+00:01:29,950 --> 00:01:36,800
+هذه عظمه .. متفتتماشي اكتر وهذه اكم عظم هذه واحد
+
+14
+00:01:36,800 --> 00:01:42,980
+اثنتين تلاتة اربع اكتر من عظمتين هذا يعني يعتبر
+
+15
+00:01:42,980 --> 00:01:47,220
+هاي مثلا كسر مفتوح في المحظات الكسر مفتوح ايش صاير
+
+16
+00:01:47,220 --> 00:01:58,400
+طالع المكان من جوا نعم حكينا من اعراض الكثور انه
+
+17
+00:01:58,400 --> 00:02:03,950
+قد لا نجد الا حاجة واحدة مثلا نجد عدم قدر علىتحريك
+
+18
+00:02:03,950 --> 00:02:09,410
+ال .. البكان او نجد ورم او نجد ألم شديد ماشي و
+
+19
+00:02:09,410 --> 00:02:15,550
+يكون فيه عنا كثر وهذه غالبا بتسير في الأطراف انه
+
+20
+00:02:15,550 --> 00:02:21,790
+بتلاقي بيسير في الشيء الا علامة واحدة و ممكن يكون
+
+21
+00:02:21,790 --> 00:02:25,930
+الدكتور مش متخصص مثلا او مش كثير يعني مر عليه
+
+22
+00:02:25,930 --> 00:02:29,890
+حالات فبتلاقيه بتعدى عليه فإذا ظل المريض يشكي من
+
+23
+00:02:29,890 --> 00:02:36,720
+هذا الألم بنروح تاني و تالتحتى يكتشفوا انه ايش في
+
+24
+00:02:36,720 --> 00:02:48,200
+عندنا مشكلة في ال .. عشان نسعف الكسر احنا
+
+25
+00:02:48,200 --> 00:02:53,020
+دورنا كمصرفين ايش نساوي تثبيت
+
+26
+00:02:53,020 --> 00:02:57,980
+العظام ممنوع نخل العظام يتحرك شوف هذه التثبيت
+
+27
+00:02:57,980 --> 00:03:01,460
+العظام بيمنع
+
+28
+00:03:01,460 --> 00:03:08,690
+حدوث مضاعفاتو بخفف الألانة كي بدأ أثبت العظام كي
+
+29
+00:03:08,690 --> 00:03:15,330
+بدأ أثبت العظام نثبت العظام طبعا بوضع ما يسمى
+
+30
+00:03:15,330 --> 00:03:20,570
+بالجابيرة في حاجة اسمها إيه الجابيرة الجابيرة عادة
+
+31
+00:03:20,570 --> 00:03:27,250
+من وين بنجيبها كل ما حولي ممكن أستخدم الجابيرة زي
+
+32
+00:03:27,250 --> 00:03:32,390
+الكرتون جرائد
+
+33
+00:03:34,710 --> 00:03:43,770
+كتب، إيد، عصات مكنسة أو عصات قشاطة، أغصان
+
+34
+00:03:43,770 --> 00:03:51,670
+الصجر، كل هذه الشغلات .. نعم؟ ممكن أجيب مثلا حرام
+
+35
+00:03:51,670 --> 00:03:56,650
+و ألفه فبصير صلب، لما ألفه و يصير صلب بصير ..
+
+36
+00:03:56,650 --> 00:04:00,760
+بشتغل جابيرةكل هذه الشغلات ممكن انا استخدمها
+
+37
+00:04:00,760 --> 00:04:04,180
+كجبيرة و ممكن انا استخدم جبيرة و مافيش عندي ولا
+
+38
+00:04:04,180 --> 00:04:09,060
+حاجة زي هيك زي كيف مش ممكن تيجي في مكان خالق او
+
+39
+00:04:09,060 --> 00:04:15,480
+بعيد عن كل البشر و مافيش ولا مكنسة ولا خشبة ولا
+
+40
+00:04:15,480 --> 00:04:19,960
+قطعة جماش ولا فيه اي حاجة كيف ممكن انا اعمل جبيرة
+
+41
+00:04:19,960 --> 00:04:25,720
+ايش هدف الجبيرة هو منع
+
+42
+00:04:25,720 --> 00:04:30,780
+حركة العضو المصابغالبا العضو المصاب إذا كان بده
+
+43
+00:04:30,780 --> 00:04:37,200
+يكون إيد أو رجل، مش هيك؟ لو حطيت إيدي جنب جسمي،
+
+44
+00:04:37,200 --> 00:04:43,820
+إيدي جنب جسمي ولفيتها، بصير مثبتة ولا لأ؟ طب و
+
+45
+00:04:43,820 --> 00:04:49,340
+الرجل؟ الرجل الأولى السليمة مع الرجل المصابة
+
+46
+00:04:49,340 --> 00:04:54,400
+وانثبتها، بصير جابيرة ولا بصيرش؟ إيش استخدمنا؟
+
+47
+00:04:56,460 --> 00:05:02,320
+استخدمنا جسم المصاب استخدمنا جسم المصاب كجبيرة
+
+48
+00:05:02,320 --> 00:05:09,340
+طبعا عشان نعمل إسعاف للكسور بدنا أي حاجة موجودة مع
+
+49
+00:05:09,340 --> 00:05:14,480
+الكسر بدنا نعالجها يعني عندي نزيف بده واقف النزيف
+
+50
+00:05:14,480 --> 00:05:20,040
+عندي جروح بده أغطي الجروح لأنه إذا حطيتش بيرة و
+
+51
+00:05:20,040 --> 00:05:25,060
+الجروح مفتوح هتأدي إلى تلوث المكان فبده أعمل غيار
+
+52
+00:05:25,870 --> 00:05:32,950
+للمكان الجروح و ثم اوضع اللى هو الجبيرة طبعا قدر
+
+53
+00:05:32,950 --> 00:05:39,370
+ما يمكن لازم مانحركش الجزء المصاب و بعدين
+
+54
+00:05:45,010 --> 00:05:48,790
+إذا في إمكانية أن أعمل إسعاف في نفس المكان يعني
+
+55
+00:05:48,790 --> 00:05:52,770
+ماحاولش أنجل المصاب إذا في إمكانية لأي مكان ثاني
+
+56
+00:05:52,770 --> 00:05:57,430
+لإن نقل المصاب لأي مكان ثاني ممكن يعمل يزيد النزيف
+
+57
+00:05:57,430 --> 00:06:03,970
+أو يزيد تهتك الأنسجة أو حتى يخليالعظام المكسورة
+
+58
+00:06:03,970 --> 00:06:10,370
+تعمل جروح في الجسم و طبعا لازم يكون كل شئ ب ..
+
+59
+00:06:10,370 --> 00:06:15,330
+برقة و بحنية زي ما اقوله مش بالعنف لإن العنف في
+
+60
+00:06:15,330 --> 00:06:21,090
+حالة الكسور بيخرب أكتر من ما بيفيد و احنا كمسعفين
+
+61
+00:06:21,090 --> 00:06:26,760
+إذا لجيت العظم خارج إلى الخارج ممنوع اترجعيهحتى
+
+62
+00:06:26,760 --> 00:06:30,400
+المفصل إذا لجيتيه مخلوق وانتكيش دراية في ترجيع
+
+63
+00:06:30,400 --> 00:06:36,300
+المفصل سبيه وبنتعامل معاه كأنه إيش فيه كسر إذا
+
+64
+00:06:36,300 --> 00:06:41,940
+لجيت مفصل مخلوق بتعامل إنه فيه كسر وبحاولش أرجع
+
+65
+00:06:41,940 --> 00:06:49,160
+المفصل حكينا عن الجبائر جونا الجبائر بنجيبها من ما
+
+66
+00:06:49,160 --> 00:06:56,000
+يوجد حولنا طبعا اليوم في جبائر معدنيةاليوم زي غطاء
+
+67
+00:06:56,000 --> 00:07:03,320
+طنجرة الخبز شوفتوها السلوك مش فيه سلوك على طنجرة
+
+68
+00:07:03,320 --> 00:07:08,640
+اللي نخبز فيها في البيت عليها سلوك زي نفس الفكرة
+
+69
+00:07:08,640 --> 00:07:12,160
+عاملين اليوم جباير بتلاقي مستشفياتنا كلها ملانة من
+
+70
+00:07:12,160 --> 00:07:16,790
+هذه الجباير بس بتشكل مستطيلبشكل حوالي سبعة إلى
+
+71
+00:07:16,790 --> 00:07:21,850
+عشرة سانتي في طول مثلا ستين سانتي تمانين سانتي
+
+72
+00:07:21,850 --> 00:07:26,690
+اربعين سانتي تلاتين سانتي هذه بنستخدمها كل جباير
+
+73
+00:07:26,690 --> 00:07:33,050
+عبارة عن قطع معدنية بنستخدمها كجباير كمان ممكن
+
+74
+00:07:33,050 --> 00:07:38,450
+نستخدم الخشب ممكن نستخدم قطع البلاستيك ممكن نستخدم
+
+75
+00:07:38,450 --> 00:07:44,990
+أي حاجة حوالينا كتب كتاب بتلفيه بتستخدميه جبيرةمش
+
+76
+00:07:44,990 --> 00:07:51,590
+موجود ولا إيش زي ما قلنا بنستخدم جسم المصاب للمصاب
+
+77
+00:07:51,590 --> 00:07:54,310
+يعني الإيجار اليمين بتعمل شبيه للإيجار الشمال
+
+78
+00:07:54,310 --> 00:08:00,370
+الإيجار الإيد بستخدمها مع الجسم فبصير عندي جبه
+
+79
+00:08:00,370 --> 00:08:06,310
+ناشي و زي ما قلنا لما نيجي ننقل مصاب بدنا ننقله
+
+80
+00:08:06,310 --> 00:08:10,510
+بهدوء إذا كان في كسر في العمود الفقري أو في الظهر
+
+81
+00:08:10,510 --> 00:08:16,750
+أو في الرجبةما بديش أخلى المصاب يتحرك بدي أحاول ما
+
+82
+00:08:16,750 --> 00:08:23,430
+استطعت أني أثبته لكن بعد ما أعمل الدور المطلوب مني
+
+83
+00:08:23,430 --> 00:08:28,690
+ايش الدور المطلوب مني؟ لما تنام على فرشة كيف بيكون
+
+84
+00:08:28,690 --> 00:08:35,690
+ركبتك على الفرشة؟ مرتفع كيف بيكون آخر الظهر؟ يعني
+
+85
+00:08:35,690 --> 00:08:38,290
+و أنا نام على الأرض بقدر أحط إيدي تحت آخر ظهري من
+
+86
+00:08:38,290 --> 00:08:46,470
+تحت ولا لأ؟ ليش؟في فراغ شكل العمود الفقري يعني مش
+
+87
+00:08:46,470 --> 00:08:53,090
+عدل غير مستقيل فبكون في مناطق فيها فراغ اذا اذا
+
+88
+00:08:53,090 --> 00:08:57,950
+انا بدي اجي اعمل اسعاف اولي لمصاب عنده كسر في
+
+89
+00:08:57,950 --> 00:09:02,190
+العمود الفقري ايش بدي اعمله بدي اثبت البني ادم
+
+90
+00:09:02,190 --> 00:09:08,740
+مايتحركش و احط في مناطق الفراغتثبيتات يعني تحت
+
+91
+00:09:08,740 --> 00:09:14,480
+الرجبة و تحت الظهر ممكن احط بشكير قطعة قماش ممكن
+
+92
+00:09:14,480 --> 00:09:20,840
+احط عمل اجيب شوية رمل و احطها ليش؟ لأن ممكن الظهر
+
+93
+00:09:20,840 --> 00:09:25,600
+تجلق يكسر زيادة العمود الفقر المكسور او ممكن
+
+94
+00:09:25,600 --> 00:09:32,240
+الرجبة نفسها تنكسر زيادة لأنه صار في عنديثجل من
+
+95
+00:09:32,240 --> 00:09:37,200
+الرجبة والراس والجسم وفيش استمرارية في العظم
+
+96
+00:09:37,200 --> 00:09:51,500
+فبنكسر وبصير عندى ما يسمى باشهدان عشان
+
+97
+00:09:51,500 --> 00:09:56,660
+نيجى نعمل إسعاف أولى لوحد مصاب بالكسر
+
+98
+00:10:00,230 --> 00:10:06,110
+لازم ان ثبت المكان المصاب عشان اثبت المكان احتاج
+
+99
+00:10:06,110 --> 00:10:13,750
+الى جابيرة تغطي المفصل قبل الكسر ومفصل بعد الكسر
+
+100
+00:10:13,750 --> 00:10:19,830
+هتصوري مثلا واحد ايده مكسورة ماشي الايد مكسورة من
+
+101
+00:10:19,830 --> 00:10:26,170
+هنا كيف بتشوفون بيحطوله جبس الذاكرين بيحطوا جبس من
+
+102
+00:10:26,170 --> 00:10:34,240
+عند كفة الايد لنص ال ايهمن نص الذراع مظبوط ليش طيب
+
+103
+00:10:34,240 --> 00:10:38,620
+عشان يثبت هذا المفصل و يثبت هذا المفصل لأنه اذا
+
+104
+00:10:38,620 --> 00:10:42,680
+هذا المفصل ماثبتش او هذا المفصل ماثبتش و بيظل في
+
+105
+00:10:42,680 --> 00:10:47,020
+حركة كل ما اتحركت العظمة بتلحمش الحاجة التانية
+
+106
+00:10:47,020 --> 00:10:51,920
+بتعمل مشاكل تبدأ تقطع في الأنسجة و تقطع في الأوطان
+
+107
+00:10:51,920 --> 00:10:59,370
+و بتعمل ألم للإنسان و بتعمله حاجات يعني كبيرة فمن
+
+108
+00:10:59,370 --> 00:11:02,190
+الحاجات اللى انا بدى اعملها بدى اثبت مفصل قبل و
+
+109
+00:11:02,190 --> 00:11:07,650
+مفصل بعد الحاجة التانى بديش احط جبيرة مباشرة على
+
+110
+00:11:07,650 --> 00:11:12,890
+المنطقة اللى بدها تنحطلها جبيرة بدي احطلها عبارة
+
+111
+00:11:12,890 --> 00:11:19,310
+عن تبطين ابطانة الابطانة ده من اين بجيبها قطعة
+
+112
+00:11:19,310 --> 00:11:26,440
+قماش قطعة قطنقطرة سفنج أي حاجة تنحط بين الجبيرة
+
+113
+00:11:26,440 --> 00:11:31,340
+الصلبة وبين جسم الإنسان لأن هذه عبارة عن شيء
+
+114
+00:11:31,340 --> 00:11:37,700
+هيعمله صدمة ويعمله احتكاك ويعمله فبقى امنع
+
+115
+00:11:37,700 --> 00:11:45,360
+الاحتكاك وامنع الجرح في منطقة اللي هو الجبيرة طبعا
+
+116
+00:11:45,360 --> 00:11:51,000
+بتعملاسعاف أول لأي حاجة، في جراح بدي أغيرله، في
+
+117
+00:11:51,000 --> 00:11:55,920
+صعوبة في التنفس بدي أساعده يتنفس، في نزيف بدي أوقف
+
+118
+00:11:55,920 --> 00:12:01,060
+النزيف، كل هذه الشغلات من واجبات المسعف في حال
+
+119
+00:12:01,060 --> 00:12:04,360
+اسعاف اللي هو الكسور
+
+120
+00:12:07,240 --> 00:12:13,320
+معناه مش لازم يعني اذا كان مصاب جنب المستشفى ولا
+
+121
+00:12:13,320 --> 00:12:18,080
+كان مصاب في مكان .. اروح اللي انا جاره اجره مكسورة
+
+122
+00:12:18,080 --> 00:12:24,020
+جار المصاب و اجره مكسورة بيعمل زيادة نزيف و زيادة
+
+123
+00:12:24,020 --> 00:12:28,220
+تهتك في الأنسجة فدائما دائما دائما اي واحد مصاب
+
+124
+00:12:28,220 --> 00:12:34,920
+بيكسر لازم اثبت منطقة الكسر قبل ما اعمله ايحركة
+
+125
+00:12:34,920 --> 00:12:41,760
+وإلا ممكن جدا أعمل زيادة تهتك وزيادة نظيفة زى ما
+
+126
+00:12:41,760 --> 00:12:46,880
+قلنا الجبائر بنجيبها من أي حاجة موجودة حوالنا من
+
+127
+00:12:46,880 --> 00:12:53,070
+إيش ممكن نجيب الجبائر قطعة كرتونبتنفع جبيرة و لا
+
+128
+00:12:53,070 --> 00:12:59,490
+بتنفعش؟ الكتاب بتنفع جبيرة و لا بتنفعش؟ قطعة قماش
+
+129
+00:12:59,490 --> 00:13:04,690
+اللفة لما تصير صلبة شوية بتنفع و لا بتنفعش؟ عصات
+
+130
+00:13:04,690 --> 00:13:11,970
+مكنسة ممكن نستخدمها جبيرة و لا لأ؟ الألواح الخشبية
+
+131
+00:13:11,970 --> 00:13:16,590
+الرفيعة أو الرقيقة ممكن نستخدمها؟ كل هذه الشغلات
+
+132
+00:13:16,590 --> 00:13:21,400
+ممكن عروف الصجر ممكن نستخدمها جبيرة و لا لأ؟و انت
+
+133
+00:13:21,400 --> 00:13:26,000
+ماشي فى الشارع و لقيت صجرة و فى عرق صجرة ممكن نقصه
+
+134
+00:13:26,000 --> 00:13:31,920
+و نستخدم جبيرة فإيش في حواليك ممكن تستخدمي جبيرة
+
+135
+00:13:31,920 --> 00:13:37,420
+لإنسان الجبيرة زى ما قلنا لازم تغطى جزء مفصل قبل
+
+136
+00:13:37,420 --> 00:13:41,460
+مفصل بعد بس انا قبل ما اعمل الجبيرةعلى الأطراف
+
+137
+00:13:41,460 --> 00:13:46,240
+بالذات لازم أجيس النبض للمصاب ليش لازم أجيس النبض
+
+138
+00:13:46,240 --> 00:13:51,780
+للمصاب؟ لأنه بعد ما أحط الجبيرة ممكن أنا ألف و
+
+139
+00:13:51,780 --> 00:13:59,240
+أضغط زيادة فيروح النبض معناته أنا عملت مشكلة فلازم
+
+140
+00:13:59,240 --> 00:14:03,340
+أجيس النبض قبل و بعد وضع الجبيرة و إلا ممكن
+
+141
+00:14:03,340 --> 00:14:09,380
+الجبيرة تكون عائق وسبب في زيادة مشاكل المصابعشان
+
+142
+00:14:09,380 --> 00:14:13,000
+أتأكد أنه في ضايل الأوعية الدموية شغالة كويس و
+
+143
+00:14:13,000 --> 00:14:17,100
+الدورة الدموية بتوصل دم للجزء المصاب لازم أجيس
+
+144
+00:14:17,100 --> 00:14:22,480
+النبض قبل و بعد وضع الجباير طب كيف أنا أستخدم
+
+145
+00:14:22,480 --> 00:14:27,280
+الجباير هذه الجباير
+
+146
+00:14:27,280 --> 00:14:33,980
+هذه في عندنا لو في كسر في عظمة اللوح عظمة اللوح
+
+147
+00:14:33,980 --> 00:14:34,520
+هذه وين
+
+148
+00:14:37,850 --> 00:14:44,410
+الكتف من ورا ماشي كيف ممكن اعملها اسعاف بالرباط
+
+149
+00:14:44,410 --> 00:14:49,630
+المثلث عرفات الرباط المثلث تحط رباط مثلث و بتثبتي
+
+150
+00:14:49,630 --> 00:14:54,470
+المنطقة و بنحط كمان رباط مثلث حوالين الايد فصارت
+
+151
+00:14:54,470 --> 00:14:59,410
+الايد ثابتة بتتحركش طب لو كان في عضمة التلقوة اللي
+
+152
+00:14:59,410 --> 00:15:04,930
+هي هذه كيف بدأ اثبتها عضمة التلقوة سيادتك وسيادة
+
+153
+00:15:04,930 --> 00:15:12,440
+الكلمستقيمة وعدلة مظبوط ليش مستقيمة وعدلة لإن في
+
+154
+00:15:12,440 --> 00:15:17,080
+عظمتين hand ردت الكتف إلى مكانه لو انكسرت واحدة
+
+155
+00:15:17,080 --> 00:15:25,200
+ممكن يصير هيك ثورات هذه بدل ما هي عدلة تصير تكسر
+
+156
+00:15:25,200 --> 00:15:30,580
+فبصير الواحد جاله هيك عشان أعدل المكان ما استطعت
+
+157
+00:15:30,580 --> 00:15:37,790
+توضعكمية من القطن تحت الإبط أو مخدة صغيرة تحت
+
+158
+00:15:37,790 --> 00:15:44,870
+الإبط أو قطعة قماشك يعني كافية تحت الإبط و يعلق
+
+159
+00:15:44,870 --> 00:15:51,300
+بينارباط مثلث ويعلق بارباط مثلث ليش؟ هذه بترجع
+
+160
+00:15:51,300 --> 00:15:56,080
+المكان الى مكان عليه مستطعناه وبتمنع زيادة انها
+
+161
+00:15:56,080 --> 00:16:02,400
+تخش اليد و تقصر المنطقة و يصير فيه تشوء طبعا عشان
+
+162
+00:16:02,400 --> 00:16:08,420
+اثبتها مظبوط ارباط مثلث بمنع انها تروح يمين وشمال
+
+163
+00:16:08,420 --> 00:16:14,210
+بمنعش بمنعفبحط كمان رباط مثلث او رباط اخر فوقه
+
+164
+00:16:14,210 --> 00:16:19,430
+فبصير عندى رباط معلق ايدي فوق تحت ورباط معنى ايدي
+
+165
+00:16:19,430 --> 00:16:26,210
+تروح داخل وخارجي واضحة الفكرة شايفين انا ايش مساوي
+
+166
+00:16:26,210 --> 00:16:35,730
+مثبت ادي بايه؟ بجسمه مثبت اديه بجسمه بمعنى انه
+
+167
+00:16:35,730 --> 00:16:44,210
+مستخدم الجسمكجبيرة ليه تثبيت اللي هو اليد المكسورة
+
+168
+00:16:44,210 --> 00:16:52,730
+كسر عظمة اللوح نفس كسر عظمة الطرق هو بنحط إيش اللي
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+169
+00:16:52,730 --> 00:17:00,210
+هي رباط مثلث ورباط مثلث آخر كسر
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+170
+00:17:00,210 --> 00:17:03,130
+عظمة العضد
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+171
+00:17:06,540 --> 00:17:13,340
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+172
+00:17:13,340 --> 00:17:19,120
+لهان بنحطله جابيرة لإنه في مكانية نركب جابيرة هان
+
+173
+00:17:19,120 --> 00:17:24,560
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+174
+00:17:24,560 --> 00:17:30,560
+مثلث ارجعوا لصورة شكل ستين صفحة مية وأربعة
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+175
+00:17:30,560 --> 00:17:36,970
+شايفاتها؟ملاحظين حطين ايش هيحط جبيرة في واحد وستين
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+176
+00:17:36,970 --> 00:17:44,470
+وستين ومحطوط ما يسمى بغباط المثلث عشان يمنع الحركة
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+177
+00:17:44,470 --> 00:17:52,890
+كسر عضمة المرفق المرفق اللي هو الكوء المرفق عبارة
+
+178
+00:17:52,890 --> 00:18:00,630
+عن ايش كام عضمة تلت عضمات ولا لا الكوء هذا عبارة
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+179
+00:18:00,630 --> 00:18:06,480
+عن تلت عضماتعظمة العلوية وعظمتين اتحاد فلو صار كسر
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+180
+00:18:06,480 --> 00:18:12,120
+في المرفق انت كمسحف مابتقدر تحدد اي عظمة مكسورة مش
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+181
+00:18:12,120 --> 00:18:17,760
+سهل بدها أشعة وبدها تشخيص فانا بدي اعتبر التلاتة
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+182
+00:18:17,760 --> 00:18:21,840
+ممكن يكون المكسورة فإيش بدي أسوي بدي أخلي اليد
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+183
+00:18:21,840 --> 00:18:26,850
+مستقيمةانه انتنيتها هنا عملت مشكلة نخلي الإيد
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+184
+00:18:26,850 --> 00:18:32,890
+مستقيمة و أثبت الإيد بالطريقة هذه غالبا مع جسم
+
+185
+00:18:32,890 --> 00:18:40,770
+الإنسان بأثبتها زي ما في شكل 62 حاطة جافيرة و نثبت
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+186
+00:18:40,770 --> 00:18:46,710
+الإيد مع اللي هو الجسم كسر
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+187
+00:18:46,710 --> 00:18:56,600
+اللي هو الساعد الساعد وينمن هاللى هالك كسر الساعد
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+188
+00:18:56,600 --> 00:19:05,340
+بنحط جبيرة زى اى مكان بنحط جبيرة او تنتين و
+
+189
+00:19:05,340 --> 00:19:15,280
+بنثبتهم و بنعلق اللى هو الايد برباط مثلث ماشي؟ كسر
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+190
+00:19:15,280 --> 00:19:23,890
+عظمة الفخدكسر عضمة الفخت من أخطر أنواع الكثور اللى
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+191
+00:19:23,890 --> 00:19:28,490
+ممكن يصير للإنسان طبعا إيش بده سببها؟ بده صدمة
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+192
+00:19:28,490 --> 00:19:35,270
+عنيفة جدا؟ أو اطلاق نار؟ أنا شوفت أكتر من حالة كسر
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+193
+00:19:35,270 --> 00:19:40,790
+الساعد كان سبب في وفاته، توفى، حد فيه كثاجن
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+194
+00:19:40,790 --> 00:19:46,500
+المغازي فشها و لا دير البلعبيا عندنا راجل في
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+195
+00:19:46,500 --> 00:19:51,780
+المغازي أجاء طلق في الفقد و روحت زورته في المستشفى
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+196
+00:19:51,780 --> 00:19:58,600
+حين الحادث و سيبته عادي جدا وصلت الدار اتصل عليه
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+197
+00:19:58,600 --> 00:20:01,920
+ابني كان هناك قال لي يا أبا الشيخ اتوفى قال يا
+
+198
+00:20:01,920 --> 00:20:05,700
+رحمة الله ماخدين بادكو كيف؟ فممكن عضمة الساعد اللي
+
+199
+00:20:05,700 --> 00:20:12,980
+.. الفقد هذه ينزف حوالي اتنين لتر في فترة قصيرة
+
+200
+00:20:13,600 --> 00:20:18,300
+لأنها كلها أوعية دموية كبيرة و ممكن تؤدي الى وفاة
+
+201
+00:20:18,300 --> 00:20:23,040
+الإنسان و ممكن نخش في صدمة نتيجة فقد الدم و نتيجة
+
+202
+00:20:23,040 --> 00:20:31,340
+الألم الشديد ايه اللي بيصير؟ اه اشي صار فيه؟ لحجوه
+
+203
+00:20:31,340 --> 00:20:37,330
+بس نزف كميات كبيرة و لحجوه طيب الحمد لله أسدمتالف
+
+204
+00:20:37,330 --> 00:20:43,130
+سلام فكسر عظمة الفقد هذه ممكن جدا تؤدي الى وفاة
+
+205
+00:20:43,130 --> 00:20:47,830
+انسان او الى فقدان كمية كبيرة من الدم و الى تشوف
+
+206
+00:20:47,830 --> 00:20:55,350
+في منطقة اللى هى الكسر كيف بنعمل اسعاف لكسر عظمة
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+207
+00:20:55,350 --> 00:21:00,810
+الفقد طبعا إذا في نزيف بنحاول نوقف النزيف وقفى
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+208
+00:21:00,810 --> 00:21:05,780
+بطريقة البترإذا مارضيش يجف كيف احنا نتعامل مع
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+209
+00:21:05,780 --> 00:21:10,560
+better ممكن اربط رباط مانع او رباط ضغط و اوقف
+
+210
+00:21:10,560 --> 00:21:17,700
+النظيف و بدي احط جبيرة تيجي من أسفل القدم الى
+
+211
+00:21:17,700 --> 00:21:24,600
+الإبط عشان اثبت هذا المفصلوالتانية من الداخل من
+
+212
+00:21:24,600 --> 00:21:31,900
+أسفل القدم إلى آخر الفخد وبثبتهم بطريقة تكون مبطنة
+
+213
+00:21:31,900 --> 00:21:36,000
+و تمام تمام و بودي على أقرب مركز طبي هذا بالنسبة
+
+214
+00:21:36,000 --> 00:21:42,420
+للفخد لكن الساق بد جبيرة تانية جبيرة من أسفل القدم
+
+215
+00:21:42,420 --> 00:21:45,500
+لأعلى الركبة و الجبيرة التانية من الداخل برضه من
+
+216
+00:21:45,500 --> 00:21:50,540
+أسفل القدملأعلى الركبة و بنثبتهم و بنبعتهم على
+
+217
+00:21:50,540 --> 00:21:59,340
+أقرب مركز طبي كسر نفس القدم قدم ممكن تنكسر بدي
+
+218
+00:21:59,340 --> 00:22:05,180
+أثبتها كيف؟ بنجيب قطعة قماش بشكير و بنلويها او
+
+219
+00:22:05,180 --> 00:22:14,300
+مخدة صغيرة او كرتونة او اذا موجود عندىالشبك اللى
+
+220
+00:22:14,300 --> 00:22:19,080
+بنستخدمه فى المستشفيات باطعجه بزاوية قائمة و بثبت
+
+221
+00:22:19,080 --> 00:22:24,660
+اللى هى القدم بحيث انه تكون ناخدة وضعها الطبيعى
+
+222
+00:22:24,660 --> 00:22:31,740
+اللى هى عبارة عن زاوية قائمة الان قد ينكسر العمود
+
+223
+00:22:31,740 --> 00:22:35,400
+الفقري كيف انت كمصرفة بدك تعرفى ان فى كسر فى
+
+224
+00:22:35,400 --> 00:22:40,240
+العمود الفقري تقدر تقوله نعم
+
+225
+00:22:41,590 --> 00:22:45,010
+بكون الإصابة فى الظهر بيقولى اللى هو وين إصابتك
+
+226
+00:22:45,010 --> 00:22:49,150
+بيقولك والله أنا ظهري حاسس أنه هويتى فى ظهري واش
+
+227
+00:22:49,150 --> 00:22:54,350
+كمان ممكن يصير بالنسبة للي عنده كسر فى العمود
+
+228
+00:22:54,350 --> 00:22:59,170
+الفقري او احتمال كسر فى العمود الفقري بيصير عنده
+
+229
+00:22:59,170 --> 00:23:04,870
+مشاكل فى الساقين فى الرجل يعني اما انه بيحس خدلان
+
+230
+00:23:04,870 --> 00:23:12,330
+فى رجله ماخدين بيت ككير او فى رجليه التنتينأو بيحس
+
+231
+00:23:12,330 --> 00:23:19,790
+عدم قدرة على حركة إجره، ولو قلتله حرك صوابع رجلك،
+
+232
+00:23:19,790 --> 00:23:23,110
+بيقدرش يحرك صوابع رجله، أي واحد من هدول اللي
+
+233
+00:23:23,110 --> 00:23:27,410
+جيتيها بيظن إنه إيش فيه كسر في العمود الفقري، إيش
+
+234
+00:23:27,410 --> 00:23:31,010
+دوري أنا كمسه في كسر العمود الفقري؟
+
+235
+00:23:34,490 --> 00:23:40,630
+خلّيه مرتاح نايم لأنه صدقوا يقعد ساعة نايم و يستمر
+
+236
+00:23:40,630 --> 00:23:45,970
+الإسعاف أحسن ما أسفه بطريقة خطأ و ينشل و يظل باجي
+
+237
+00:23:45,970 --> 00:23:53,330
+عمره يعاني من إيش؟ من أثار الحادث فبخلّيه مرتاح و
+
+238
+00:23:53,330 --> 00:23:57,700
+باجي أنا على المناطق الفاضيةاللي هي تحت الظهر في
+
+239
+00:23:57,700 --> 00:24:04,960
+أسفله و تحت الرجبة و بمليها بحيث انه تسير ماتضغطش
+
+240
+00:24:04,960 --> 00:24:10,560
+على جسمه و تنكسر بزيادة كي بمليها قطعة قماش بشكير
+
+241
+00:24:10,560 --> 00:24:17,800
+قطعة قطن بكت قطن مافيش ولا حاجة شوية رملالرمل اللى
+
+242
+00:24:17,800 --> 00:24:23,620
+حوليا بعبى هذه المنطقة عشان تظل داعمة العمود
+
+243
+00:24:23,620 --> 00:24:28,760
+الفقري و يظل العمود الفقري ماخد شكله الطبيعى ماشي
+
+244
+00:24:28,760 --> 00:24:33,140
+طب انا برضه المرة الأخيرة بنصح انه ايش مانحاولش
+
+245
+00:24:33,140 --> 00:24:38,840
+ننجله لكن قد نضطر ان ننجله كيه بنضطر اذا ننجله اذا
+
+246
+00:24:38,840 --> 00:24:44,120
+بدنا ننجله بدنا نخليه مايتحركش ولا حركة جسمه جسمه
+
+247
+00:24:44,120 --> 00:24:52,770
+يكون شك في واحد زى كيفيعني انشبه انه مثلا هذه
+
+248
+00:24:52,770 --> 00:25:00,050
+الشنطة اللي هي البني آدم كيف بدى اتعامل معها؟
+
+249
+00:25:00,050 --> 00:25:08,130
+اتعامل معها كالقاتل بيجوا تلت تمفاربيجي عند المصاب
+
+250
+00:25:08,130 --> 00:25:12,070
+واحد في نصه و واحد عند جريه او عند وسطه و واحد عند
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+251
+00:25:12,070 --> 00:25:17,150
+راسه و بيجي واحد عند الراس على الشج هذه و بيشد
+
+252
+00:25:17,150 --> 00:25:22,610
+الراس عشان قد يكون كسر في وين؟ في الرقبة اللي
+
+253
+00:25:22,610 --> 00:25:26,750
+بيشتغل عند الراس بيكون الأكتر خبرة و دراية و هو
+
+254
+00:25:26,750 --> 00:25:34,290
+اللي بد الأوامر الآن ايش بيصير؟ هو بيقول لفه معايا
+
+255
+00:25:35,180 --> 00:25:42,060
+بكون احنا محضرين لوح خشب هان موجود لوح خشب و بنيجي
+
+256
+00:25:42,060 --> 00:25:45,840
+احنا التلاتي اللي هانا بنسحب و هو بيبقى ماسك
+
+257
+00:25:45,840 --> 00:25:51,900
+الرجبه و بيجي اللي في النص منهم بيسحب الخشب تحت
+
+258
+00:25:51,900 --> 00:25:56,920
+المصاب واحد اتنين تلاتي بنرجع الحين صار المصاب وين
+
+259
+00:25:56,920 --> 00:26:05,150
+فوق لوح الخشبماشي بنثبته بنربطه يعني بنثبته بنحط
+
+260
+00:26:05,150 --> 00:26:11,160
+تحت الرجبدعمات و تحت الظهر و بنربط بالإكشاتات و
+
+261
+00:26:11,160 --> 00:26:14,560
+بنرفع اللي هو الشيال إلى أين لكن برضه لازلت بقول
+
+262
+00:26:14,560 --> 00:26:20,200
+انه CB اللي مكسور بالعمود الفقري إذا استطعتي حتى
+
+263
+00:26:20,200 --> 00:26:25,900
+يأتي المسعفين مع ملء الفراغات اللي في الرجبة و تحت
+
+264
+00:26:25,900 --> 00:26:32,600
+الظهر واضح؟ هذا بالنسبة لإيش؟ لالإنسان اللي عنده
+
+265
+00:26:32,600 --> 00:26:40,070
+كسر في العمود الفقريفي اي سؤال؟
+
+266
+00:26:40,070 --> 00:26:45,310
+احنا
+
+267
+00:26:45,310 --> 00:26:51,750
+الآن حكينا عن الكثور فقط، مظبوط؟ احنا قلنا ان في
+
+268
+00:26:51,750 --> 00:26:58,490
+حاجة اسمها مفاصل المفاصل هذه ممكن يصير فيها خلممن
+
+269
+00:26:58,490 --> 00:27:01,250
+الخلل اللى بيصير في المفاصل انه ممكن يصير حاجة
+
+270
+00:27:01,250 --> 00:27:06,750
+اسمها الوثي او القصة او اللي مال ايدك والله انقصعت
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+271
+00:27:06,750 --> 00:27:13,730
+ايش انقصعت او ايش التوت او ايش الوثي مين تقولنا
+
+272
+00:27:13,730 --> 00:27:24,150
+ايش اللي او القصة او الوثي مش
+
+273
+00:27:24,150 --> 00:27:24,590
+ثاني
+
+274
+00:27:29,110 --> 00:27:37,430
+بتكون مش مقصودة بس بتوجه طب إيه سبب الوجاق؟ المفصل
+
+275
+00:27:37,430 --> 00:27:43,010
+بيطلع من مكانه و بيرجع نتيجة شد زيادة على المفصل
+
+276
+00:27:43,010 --> 00:27:48,760
+فتلاقي المفصل بيطلع من مكانه و بيرجعهذا بنسمي قصع
+
+277
+00:27:48,760 --> 00:27:53,440
+أو لي أو وثي طبعا لما يطلع المفصل من مكانه ويرجع
+
+278
+00:27:53,440 --> 00:27:59,200
+بيصير ورعب بيصير ألم بيصير صعوبة حركة كل هذه
+
+279
+00:27:59,200 --> 00:28:04,740
+الشغلات بتصير ايش دوري انا كمسعف دوري كمسعف انا
+
+280
+00:28:04,740 --> 00:28:09,900
+اني بحطله جبيرة عادي كأنه كسر و بتفيد كتير كتير لو
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+281
+00:28:09,900 --> 00:28:16,100
+حطيتي كامدات ميه باردة كامدات تلج ماشي فال .. ال
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+282
+00:28:16,100 --> 00:28:22,540
+ليأو الوثي أو القصة خروج المفصل من مكانه وعودته
+
+283
+00:28:22,540 --> 00:28:27,020
+إلى حالته الصحيحة وبعد هيك طبعا بيصير مشكلة في
+
+284
+00:28:27,020 --> 00:28:33,660
+الألياف وفي الأوتار وكذا فبدنا نحطله كجابيرة
+
+285
+00:28:33,660 --> 00:28:38,580
+مؤقتة و بنحطله كمدات ميه باردة و بنبعته على أقرب
+
+286
+00:28:38,580 --> 00:28:44,960
+مركز طبي طبعا المشكلة الوثي هذا و القصة انه بتظل
+
+287
+00:28:44,960 --> 00:28:51,790
+الحكاية تتكرربتظل تتكرر ايش يعني تتكرر يعني اليوم
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+288
+00:28:51,790 --> 00:28:56,070
+فلت المفصل كمان يومين تلاتة بتلاقي فلت تاني كمان
+
+289
+00:28:56,070 --> 00:29:00,310
+اربع خمس ايام قبل كام يوم يمكن يومين تلاتة تقريبا
+
+290
+00:29:00,310 --> 00:29:06,030
+اجاني حد بيشكر نفس القصة بيقولي انا الاصبع بيظله
+
+291
+00:29:06,030 --> 00:29:10,810
+كل يوم و التاني و هو يجعلى فبقوله اكيد بتأثر بنخلع
+
+292
+00:29:10,810 --> 00:29:16,390
+قاللي اه ايش الحل قولتله الحل بده يتثبت لمدة اقل
+
+293
+00:29:16,390 --> 00:29:22,470
+حاجة تلت اسابيعبدو يثبت كأنه مجبّص تلت أسبيه ليش؟
+
+294
+00:29:22,470 --> 00:29:26,590
+في التلت أسبيه هذه بترتاح العضلات و بترتاح الأوثار
+
+295
+00:29:26,590 --> 00:29:30,310
+و بترجع لوضعها الطبيعي لكن سبيها زي ما هي بيظل كل
+
+296
+00:29:30,310 --> 00:29:35,850
+يوم التاني و هي فالته صارت ضعيفة الحاجة التانية أن
+
+297
+00:29:35,850 --> 00:29:42,510
+أول ما تسير الحدث حطي كم ده التلج حطيكمداد التلج
+
+298
+00:29:42,510 --> 00:29:46,770
+لأنه كمداد التلج بتخفف الورعم و بتخفف الألم و
+
+299
+00:29:46,770 --> 00:29:50,370
+بتخفف النزيف إذا صار بالدوسير نزيف هذا بالنسبة
+
+300
+00:29:50,370 --> 00:29:57,390
+للوثين في حاجة ثانية اللي هي خلق المفصل إيش بفرق
+
+301
+00:29:57,390 --> 00:30:03,010
+خلق المفصل عن الوثين بفرق؟
+
+302
+00:30:03,010 --> 00:30:09,410
+إيش بفرق؟ خلق المفصل بطلع المفصل من المكان و بضل
+
+303
+00:30:09,410 --> 00:30:16,300
+طالعبرجعش ايش بيصير لما يطلع المفصل من مكانه برجعش
+
+304
+00:30:16,300 --> 00:30:20,780
+بيصير قلم و بيصير ورم و بيصير كذا لكن اهم حاجة
+
+305
+00:30:20,780 --> 00:30:25,540
+بتصير او اهم عرض من عراض خلق المفصل انه بيصير فقد
+
+306
+00:30:25,540 --> 00:30:29,800
+كامل للحركة في العضو العضو المفصل اللي بيتخلق
+
+307
+00:30:29,800 --> 00:30:34,460
+بيبطل يتحرك ولا بيقدر يحركه اي حركة خالص لان
+
+308
+00:30:34,460 --> 00:30:39,190
+المفصل من الأشياء اللي بتساعد عليهالحركة فمن
+
+309
+00:30:39,190 --> 00:30:46,970
+الشغلات هذه اللي هو ايش فقد كامل للحركة طبعا بيصير
+
+310
+00:30:46,970 --> 00:30:50,950
+ألم بيصير ورم بيصير تشوه و بيصير تغير في لون
+
+311
+00:30:50,950 --> 00:30:57,630
+المكان كيف بننصفه هذا زي و زي الكسر البسيط بنعمله
+
+312
+00:30:57,630 --> 00:31:02,810
+جبيرة و بنثبته و بنوديه على أقرب مركز طبي أنا إذا
+
+313
+00:31:02,810 --> 00:31:07,810
+بعرفش أرجع المفصل برجعوشلكن اذا في عندك خبرة بتعرف
+
+314
+00:31:07,810 --> 00:31:12,710
+ترجعيه فاش مشكلة انه نرجعه صدقوا انه يعني اذا
+
+315
+00:31:12,710 --> 00:31:16,370
+ماكانش فيه خبرة بيخرب الواحد اكتر من ما بيعمر
+
+316
+00:31:16,370 --> 00:31:20,370
+فبنان علي اللي بيعرفش يعني فاسألوا اهل الذكري ان
+
+317
+00:31:20,370 --> 00:31:26,310
+كنتم لا تعلموا نسيب الشغل للناس اللي متخصصين في
+
+318
+00:31:26,310 --> 00:31:32,430
+عندنا الحين عادي اسمها الشد العضلي او التمزق الشد
+
+319
+00:31:32,430 --> 00:31:40,320
+العضلي او التمزقيعني قد يكون أكتر إذاعا
+
+320
+00:31:40,320 --> 00:31:49,060
+و ألما و مدة علاج من الكسر الشد العملي يعني بمعنى
+
+321
+00:31:49,060 --> 00:31:57,180
+أنا أذكر يوم من الأيام في كان واحد بيقصنا بيشتغل
+
+322
+00:31:57,180 --> 00:32:03,390
+بيتدربفمن ضمن التدريب حطوه على سطح حوالي أربعة متر
+
+323
+00:32:03,390 --> 00:32:12,310
+و حطوه لهم و مالتهت و قالوله ايش امشي فطت قالولهم
+
+324
+00:32:12,310 --> 00:32:21,610
+ايش فطت الساق التاني واحد من الرجلين فيها التهاب و
+
+325
+00:32:21,610 --> 00:32:26,530
+كان بتعالج إليها و بدي يتمثل للشفاء بدي يتمثل
+
+326
+00:32:26,530 --> 00:32:31,830
+للشفاءفالراجل اجتهد قال للمدرب يا أخوي انا أجري
+
+327
+00:32:31,830 --> 00:32:35,610
+بتجاني قال انا بعرفش انا مش شغلتي روح جيب وارقة من
+
+328
+00:32:35,610 --> 00:32:39,850
+الطبيب الوحدة و بعافيك من المدرس اما انا مابقدرش
+
+329
+00:32:39,850 --> 00:32:45,630
+كلامه صح ولا لأ فراح اجتهد ايش ساوي أخينا أخينا
+
+330
+00:32:45,630 --> 00:32:51,830
+بده فط من سطح حوالي أربعة متر على رمل بدل مايفط
+
+331
+00:32:51,830 --> 00:32:55,890
+على رجليه تنتين قالك بده فط على واحدة أحمل عليها
+
+332
+00:32:55,890 --> 00:33:00,540
+لليش على التنتينفات وماحمدش على رجل المصابة
+
+333
+00:33:00,540 --> 00:33:05,420
+الأولانية حاومة على هذه التوت رجله بدل ما هي ماشي
+
+334
+00:33:05,420 --> 00:33:10,580
+عادل صارت عوجة هيك على الجامعة هذا اللي هو التمزق
+
+335
+00:33:10,580 --> 00:33:15,440
+العضلي هذا الشد العضلي او التمزق العضلي طبعا اتصل
+
+336
+00:33:15,440 --> 00:33:23,370
+عليافساعدت حالي و روحت كان هناك في دكتور قاعد في
+
+337
+00:33:23,370 --> 00:33:27,570
+ال .. الوحدة فقلتله صاحيح هذا يعني الشد العضلي
+
+338
+00:33:27,570 --> 00:33:31,610
+بحتاج إلى عناية طويلة و بده فترة طوية قال صح كلامك
+
+339
+00:33:31,610 --> 00:33:37,150
+اللي انا الشاب هذا حطناله جبس عساس نمنع حركتها و
+
+340
+00:33:37,150 --> 00:33:40,890
+بعد تلتر .. تلتر بعصبية هيك الراجل ماتحملش الجبس
+
+341
+00:33:40,890 --> 00:33:47,760
+فكه فكه و صار يمشي عليها بمشيه بس في ألممرجعتش الى
+
+342
+00:33:47,760 --> 00:33:53,300
+وضعها ايش؟ الطبيعى، بعد فترة مثلا بده راجع يروح
+
+343
+00:33:53,300 --> 00:33:58,300
+يحط الجبس، يركب جبس جديد ويروح يراجع وضل يا عم
+
+344
+00:33:58,300 --> 00:34:01,780
+بصحى شاكى يا .. يقولك ابصرش .. والآن ياله حوالي
+
+345
+00:34:01,780 --> 00:34:07,280
+عشر سنوات، لليوم اجرب تجعب، لليوم بحطلها مشد،
+
+346
+00:34:07,280 --> 00:34:13,750
+لليوم بقذ عليها، ليش؟ما أخدتش الوقت الكافي لإنه
+
+347
+00:34:13,750 --> 00:34:19,530
+ترجع الأوتار وترجع العضلات وترجع ال .. الوضع العام
+
+348
+00:34:19,530 --> 00:34:23,550
+إلى ما كان عليه قبل الإصابة وبناء عليه طبعا الشد
+
+349
+00:34:23,550 --> 00:34:30,270
+العضلي أو التمزق بياخد يعني اهتمام كثير لأنه ممكن
+
+350
+00:34:30,270 --> 00:34:35,390
+يكون مؤذي جدا من إسعافاتهم كمان إن هذا بيحط مكملات
+
+351
+00:34:35,390 --> 00:34:41,300
+تلج على المطرح حين ما يصير التمزق العضليبدنا نحط
+
+352
+00:34:41,300 --> 00:34:45,980
+كمدات دائما .. دائما الإصابة الحديثة دائما حطيلها
+
+353
+00:34:45,980 --> 00:34:50,900
+كمدات تلج ماتحطيش ميه سخنة كمدات السخنة في الإصابة
+
+354
+00:34:50,900 --> 00:34:57,000
+الجديدة إيش بتعمل؟ بتزيد النظيف بتزيد النظيف و
+
+355
+00:34:57,000 --> 00:35:01,100
+بتزيد الورم و بتزيد القلم و عليها بدنا نحط كمدات
+
+356
+00:35:01,100 --> 00:35:09,600
+ميه باردة في حد عنده أي سؤال؟ في أي سؤال؟الان
+
+357
+00:35:09,600 --> 00:35:14,780
+بننتقل الى موضوع انقاذ
+
+358
+00:35:14,780 --> 00:35:23,900
+ونقل المصاب ناشي؟ انقاذ ونقل المصاب كيف
+
+359
+00:35:23,900 --> 00:35:32,520
+بدنا نستفيد من هذا الموضوع؟ ايش يعني اخلاق؟ اخلاق
+
+360
+00:35:32,520 --> 00:35:36,080
+او انقاذ ونقل المصاب ايش يعني بنقصد فيه؟
+
+361
+00:35:41,450 --> 00:35:45,310
+تسمعوش انتوا فى المؤسسات الحكومية و فى الجيش و
+
+362
+00:35:45,310 --> 00:35:52,270
+الشرطة ايش مالكوا يا جماعة قال اليوم فى إخلاق ها؟
+
+363
+00:35:52,270 --> 00:35:57,190
+لأ مش بروحوش ببعدوا عن المكان هم بروحوا بدوموا بس
+
+364
+00:35:57,190 --> 00:36:01,510
+بدل ما احنا فى الغرفة هذه بنسير فى الممر مظبوط بدل
+
+365
+00:36:01,510 --> 00:36:06,890
+ما احنا هيك قاعدين حوالى عشر تنفار بيسير كل واحد
+
+366
+00:36:06,890 --> 00:36:13,610
+فى شجة واخدين بالكوا كيف الإخلاقاللي هو البعد عن
+
+367
+00:36:13,610 --> 00:36:19,470
+المنطقة اللي فيها .. اللتي فيها خطر او المتوقع
+
+368
+00:36:19,470 --> 00:36:23,610
+حدوث خطر هو دول الجيش لما بيخلوا او الشرطة لما
+
+369
+00:36:23,610 --> 00:36:30,050
+بيخلوا بيكون عندهم خطر عنيدها لكن ايش بيكون؟ متوقع
+
+370
+00:36:30,050 --> 00:36:33,890
+حدوث خطر تيجي الجيش الإسرائيلي او الطيارات
+
+371
+00:36:33,890 --> 00:36:37,730
+الإسرائيلية تضرب تقصف المكان فبصير توقع حدوث خطر
+
+372
+00:36:37,730 --> 00:36:43,650
+فبيبعدوافالتوقع حدوث الخطر او وجود الخطر بيدينى
+
+373
+00:36:43,650 --> 00:36:50,730
+اهمية انه لازم اخلى المكان وين المناطق الخطيرة
+
+374
+00:36:50,730 --> 00:36:56,270
+اللى انا لازم اخلى منها فى عدة مناطق خطيرة لازم
+
+375
+00:36:56,270 --> 00:36:59,730
+اخلى منها ايش المناطق الخطيرة اللى بتاخلى منها
+
+376
+00:36:59,730 --> 00:37:04,830
+مناطق الحروق منطقة فيها حريق
+
+377
+00:37:08,220 --> 00:37:14,280
+ليش بده اخليها طيب صح بس ليش بده اخليها متوقع
+
+378
+00:37:14,280 --> 00:37:22,940
+حدوث خطر انا بحقلي منطقة فيها خطر الآن او متوقع
+
+379
+00:37:22,940 --> 00:37:27,280
+كمان شوية تيجي طيارة او دباب تقصف المكان فصار
+
+380
+00:37:27,280 --> 00:37:33,480
+متوقع فالمناطق اللي فيها خطر باخليها المتوقع حدوث
+
+381
+00:37:33,480 --> 00:37:37,600
+خطر فيها باخليها كمان ايش المناطق اللي باخليها
+
+382
+00:37:40,710 --> 00:37:45,950
+مناطق الحريق صار نار وولعت نار الحين اللي اصلا مع
+
+383
+00:37:45,950 --> 00:37:49,290
+الله احنا قاعدين هنا صار شوية نار كل كم بيصير
+
+384
+00:37:49,290 --> 00:37:54,970
+انسرخن، مظبوط؟ مش بتفكرين كيف نسوي و نشرد، لأ و
+
+385
+00:37:54,970 --> 00:37:57,870
+بتروحين تجرن اللي انت واقفات على الباب اللي هو
+
+386
+00:37:57,870 --> 00:38:02,930
+الباب مش راضي يفتح، مظبوط؟ مش هيك بيصير؟ عشان هيك
+
+387
+00:38:02,930 --> 00:38:07,370
+المناطق اللي زي هذه المناطق الكبيرة الاصل الاصل ان
+
+388
+00:38:07,370 --> 00:38:13,070
+بوابة يفتحين براالأصل أنه بوابة إيش يفتحان برا
+
+389
+00:38:13,070 --> 00:38:16,930
+تصورها الحين فيه هنا خمسمائة واحد ولا متين واحد و
+
+390
+00:38:16,930 --> 00:38:21,650
+كلنا صار مشكلة و جينا نجري على الباب إيش بيصير؟
+
+391
+00:38:21,650 --> 00:38:27,210
+لكن لو أن الباب بيفتح لبرا مجرد واحد بس يحط إيده
+
+392
+00:38:27,210 --> 00:38:32,250
+على الإيد بيفتح و بنطلع فالمناطق اللي فيها حروق
+
+393
+00:38:32,250 --> 00:38:38,400
+مثلا بدها إخلاء لأن الحرق ممكن يمتدكمان الحريق
+
+394
+00:38:38,400 --> 00:38:44,260
+جولنا من مقاطره انه بيعمل انهيارات ماشي المناطق
+
+395
+00:38:44,260 --> 00:38:50,300
+اللي حرارتها عالية او منخفضة تحتاج الى اخلاق انا
+
+396
+00:38:50,300 --> 00:38:54,980
+جولنا اذا واحد بيشتغل في منطقة حرارتها عالية واصيب
+
+397
+00:38:54,980 --> 00:39:01,100
+بضربة حرارة وين بنسافره؟ اول حاجة بنساويها ايش؟
+
+398
+00:39:01,100 --> 00:39:07,520
+بنطلعه من المنطقة السخنة ولا لأ؟طلعوا من المنطقة
+
+399
+00:39:07,520 --> 00:39:11,200
+السخنة الى منطقة حرارتها محتملة او حرارتها طبيعية
+
+400
+00:39:11,200 --> 00:39:16,260
+فالمناطق دول الحرارة العالية جدا بنجيب منها كمان
+
+401
+00:39:16,260 --> 00:39:23,360
+التلاجة او المنطقة المنخفضة للحرارة قمة جبل بضليش
+
+402
+00:39:23,360 --> 00:39:26,400
+اسعف واحد على قمة جبل و الدنيا شتاء اربعانية و
+
+403
+00:39:26,400 --> 00:39:32,180
+التلج داب بالبلد هسير زيهحانه هسير زيه، كمان التلج
+
+404
+00:39:32,180 --> 00:39:38,500
+سهل انه مايظلش في مكانه و يصير فيه انهيار فالمناطق
+
+405
+00:39:38,500 --> 00:39:42,340
+ذات الحارة الحرارة الزائدة او الحرارة المتخفضة
+
+406
+00:39:42,340 --> 00:39:48,020
+كمان يا صبايا المناطق المرتفعة اللي فيها هوا،
+
+407
+00:39:48,020 --> 00:39:52,360
+تيارات الهوا حين انت في الشتاء او في اليوم العادي
+
+408
+00:39:52,360 --> 00:39:59,310
+اقفع على السطح فوق الرابع، شو بيصير؟بصير هوى جدا و
+
+409
+00:39:59,310 --> 00:40:03,310
+بتبقى الدنيا شوب و أنت سجان فهذه منطقة برضه بدها
+
+410
+00:40:03,310 --> 00:40:08,230
+إخلاء مفهش أعمل إسعاف فيها من المناطق اللي بكون ..
+
+411
+00:40:08,230 --> 00:40:14,370
+بدي أخليها كمان المنطقة اللي فيها كهربا جاطة سلك
+
+412
+00:40:14,370 --> 00:40:18,410
+كهربا هو المنطقة الجريبة أو واحد مصاب بالكهربا إن
+
+413
+00:40:18,410 --> 00:40:22,910
+ضلينا مسكينه بسلك الكهربا بيخرب بيته و لو أجى واحد
+
+414
+00:40:22,910 --> 00:40:28,640
+كمان صاب البني آدم بينصاب هو أيضابالكهربا والتانين
+
+415
+00:40:28,640 --> 00:40:35,060
+ممكن يموتوا من فابد أخلي البني آدم أو أبعد الكهربا
+
+416
+00:40:35,060 --> 00:40:39,020
+عنه كيف ممكن أبعد الكهربا عنه أو أخليه يا بفصل
+
+417
+00:40:39,020 --> 00:40:46,840
+الأمانيا اما ايش بجيب قطعة مادة غير قابلة للتوصيل
+
+418
+00:40:46,840 --> 00:40:52,160
+زي الخشب الجاف او البلاستيك و بزيح السلك هو بدي
+
+419
+00:40:52,160 --> 00:40:56,460
+يعني بس حاجة بسيطة بزيح السلك عن المكان و بأبعد
+
+420
+00:40:56,460 --> 00:41:02,160
+المصاب عن اللي هي مكان الخطورة
+
+421
+00:41:02,160 --> 00:41:10,420
+من المناطق الخطرة جدا الشارع العالم ولا لأمناطق
+
+422
+00:41:10,420 --> 00:41:14,460
+سير السيارات الان انا ماشي فيها الليل او في انها
+
+423
+00:41:14,460 --> 00:41:18,360
+هو عارف ان هالخط هذا يابا يما اكوسه مش فيه ولا
+
+424
+00:41:18,360 --> 00:41:23,380
+مشكلة فببقى ماشي سبعين تمانين مية هوب اللي فيه في
+
+425
+00:41:23,380 --> 00:41:28,040
+وسط قدامي اللي الواحد متمدل بلحق شجر فممكن جدا
+
+426
+00:41:28,040 --> 00:41:32,140
+خاصة اذا في منعطف او في اش زي ذلك فممكن جدا ادهسه
+
+427
+00:41:32,140 --> 00:41:37,740
+و ادهس مين المسعف فبكل بساطة هذا جديش احتاج نجل
+
+428
+00:41:41,260 --> 00:41:46,300
+شبراين .. شبراين بس عن الأسفل بسير على الرصيف و لا
+
+429
+00:41:46,300 --> 00:41:51,020
+لأ خمسين صانطي على نص متر يكفن أنه يسير على الرصيف
+
+430
+00:41:51,020 --> 00:41:56,500
+و يظل الطريق فاتحة و ماشية فبمعنى المناطق الخطيرة
+
+431
+00:41:56,500 --> 00:42:02,280
+هذه كلها تحتاج إلى إخلاء .. إيش فيه كمان مناطق
+
+432
+00:42:02,280 --> 00:42:06,100
+ممكن تسير فيها تحتاج إلى إخلاءات؟
+
+433
+00:42:09,700 --> 00:42:15,480
+المناطق اللي فيها غازات ثامنة أو فيها مكان دوار
+
+434
+00:42:15,480 --> 00:42:22,560
+ممكن يمسك البني آدم يعني الآن فيه ابخرة و الأوضة
+
+435
+00:42:22,560 --> 00:42:27,360
+ملانة بغازات ثامنة و أنا بدي أجي أسعف طب مانحن
+
+436
+00:42:27,360 --> 00:42:31,460
+خانج مع الإنسان اللي قاعد يوا و لا لأ؟ طب في عندي
+
+437
+00:42:31,460 --> 00:42:39,740
+مكينة قاعدة بتقص خشب منشار و واحد إيده انجرحتطب
+
+438
+00:42:39,740 --> 00:42:42,540
+إذا جيت أسف و أنا جنب المنشرار ماني ممكن يعني يا
+
+439
+00:42:42,540 --> 00:42:47,680
+أكمل عليها يا أنجرح زيه فالمكائن الدوارة بتشوف
+
+440
+00:42:47,680 --> 00:42:52,160
+الستات اللي بيروح يتمرجح على ال .. في ال ..
+
+441
+00:42:52,160 --> 00:42:59,000
+الملاهي في الملاهي تصور صبي صغيرة و شعرها طويل و
+
+442
+00:42:59,000 --> 00:43:05,060
+سايح زي ما بيقوله و أجت جنب المكان إيش هيصير فيها؟
+
+443
+00:43:05,060 --> 00:43:09,660
+هيشدها المكان و هيخلع الشعر من الرأسمحصلت مش
+
+444
+00:43:09,660 --> 00:43:15,100
+محصلتش انه شد الشعر و خلق الشعر من الرأس مع الجلد
+
+445
+00:43:15,100 --> 00:43:20,120
+فهذه المناطق كلها تحتاج إلى وين؟ إلى إخلاء فكل
+
+446
+00:43:20,120 --> 00:43:25,120
+المناطق يعني اللي انت شايفها تحتاج إلى إخلاء يجب
+
+447
+00:43:25,120 --> 00:43:29,160
+أن نخلق نفسنا منها شواطئ البحار و الأنهار و
+
+448
+00:43:29,160 --> 00:43:34,400
+الوديان تحتاج إلى إخلاء ولا لأ؟ طب ليش؟
+
+449
+00:43:39,350 --> 00:43:42,570
+شواطئ البحار و الانهار و الوديان تحتاجي لاخلاق ولا
+
+450
+00:43:42,570 --> 00:43:50,390
+لأ؟ تحتاجي ليش؟ اه انا بدي اسعف واحد جامب صار عنده
+
+451
+00:43:50,390 --> 00:43:56,210
+حادث على حفة البحار او على حفة واد في ميه او على
+
+452
+00:43:56,210 --> 00:44:02,430
+حفة نهر اسعف و اظل قاعد ولا ابعده؟ لازم اخليه عن
+
+453
+00:44:02,430 --> 00:44:05,450
+الحفة ليش طيب؟
+
+454
+00:44:08,750 --> 00:44:17,830
+مكان خطر، إيش القطورة في ال .. الوادي هات؟ انتوا
+
+455
+00:44:17,830 --> 00:44:22,070
+ماذا ذاكرين من فترة بسيطة كان في .. في مصر مباني
+
+456
+00:44:22,070 --> 00:44:29,010
+هالت في .. لأنه في مي من تحتها بتمشي؟ أه ذاكرينها؟
+
+457
+00:44:29,010 --> 00:44:33,990
+هالت وماتوا ناس كتير إيش القصة؟ القصة إنه المي
+
+458
+00:44:33,990 --> 00:44:39,660
+بتخلي الأرض رخوةبصير الأرض حلال فبصير الأرض يعني
+
+459
+00:44:39,660 --> 00:44:44,800
+مش متماسك هي بتجعل الآن في عندي نهر واد غزي هذا
+
+460
+00:44:44,800 --> 00:44:48,940
+إذا كانت تتذكروه كان واد غزي بتبجي السيارة فيه زي
+
+461
+00:44:48,940 --> 00:44:54,920
+ما أنا ماسك ال .. هذي بتجري و حاملها و بتفجر فيه
+
+462
+00:44:54,920 --> 00:44:58,540
+في واد غزي هذا اللي شايفته بجي سير المي فيه يعني
+
+463
+00:44:58,540 --> 00:45:03,880
+عالي جدا فكان بحمل السيارة و بحمل .. كنا نيجي
+
+464
+00:45:03,880 --> 00:45:11,000
+نتفرج عليهكان في بيارة جوا كل شوية اللى هي ماكل ال
+
+465
+00:45:11,000 --> 00:45:16,500
+.. الميه ماكل الطينة ومنازل شجرة و تسير الشجرة
+
+466
+00:45:16,500 --> 00:45:21,700
+تعوم فى وين فكمان ممكن تاكل ال .. الطينة تتاكل و
+
+467
+00:45:21,700 --> 00:45:27,000
+يكون مصعف و مصاب موجودين يقعوا فى الواد بتزهلقوا و
+
+468
+00:45:27,000 --> 00:45:30,280
+بقعوا فى الواد و بموتوا فاحنا يبقى لازم نبعد
+
+469
+00:45:30,280 --> 00:45:35,170
+المصاب عن منطقة البحروانت قاعد على جنب البحر
+
+470
+00:45:35,170 --> 00:45:39,990
+احيانا بيجي ع بالك تبلي ليجريك بالميه، مظبوط؟
+
+471
+00:45:39,990 --> 00:45:47,710
+ثواني إلا بوج كبير جاي لامغطياكي، مظبوط؟ فلو كان
+
+472
+00:45:47,710 --> 00:45:52,990
+في واحد قاعد بتسافي فيه وغططه الميه، هنقجه فكل
+
+473
+00:45:52,990 --> 00:45:55,910
+الشغلات هذه يعني شواطئ البحار، شواطئ النهار
+
+474
+00:45:55,910 --> 00:45:59,850
+المنطقة القايلة للسقوط، الانهيارات، ال .. ال .. كل
+
+475
+00:45:59,850 --> 00:46:05,280
+هذه الشغلات تحتاج إلى أنه إيش؟ننقل المصاب منها
+
+476
+00:46:05,280 --> 00:46:14,320
+منطقة فيها مثلا سموم وين بنلاقي سموم في المزارع
+
+477
+00:46:14,320 --> 00:46:18,440
+عارفين الحمامات اللي بيسموها حمامات هاد النيلون
+
+478
+00:46:18,440 --> 00:46:23,240
+ايش بيسموها هي اللغة العربية الدفيئات بيسموها
+
+479
+00:46:23,240 --> 00:46:26,820
+الدفيئات الزراعية الدفيئات الزراعية هذه كيف
+
+480
+00:46:26,820 --> 00:46:31,220
+بيشتغلوا فيها بيشتغلوا فيها برشوا فيها مواد
+
+481
+00:46:31,220 --> 00:46:37,320
+كيميائية خطيرةمواد كيميائية عشان تنمي الزرق ومواد
+
+482
+00:46:37,320 --> 00:46:41,720
+كيميائية عشان تقتل الحشرات او تقتل اللي هي
+
+483
+00:46:41,720 --> 00:46:47,900
+الطفيليات او تقتل الامراض فالصورة انه مسكرا لدافئة
+
+484
+00:46:47,900 --> 00:46:52,960
+هادي و واحد ملاني من المواد هذا وانخنج عشان انا
+
+485
+00:46:52,960 --> 00:46:58,960
+اسف و لازم وين اوديه لازم ااخده على برع بحيث انه
+
+486
+00:46:58,960 --> 00:47:06,790
+ايش هو انا ويا نتنفس هو صحولا لأ مناطق الحروق اللى
+
+487
+00:47:06,790 --> 00:47:10,730
+فيها دخن اللى .. مناطق اللى فيها حروب .. مناطق
+
+488
+00:47:10,730 --> 00:47:15,510
+اللى فيها غاز تبع اليهود انا بجيت زمان اطلع مع
+
+489
+00:47:15,510 --> 00:47:21,910
+الإسعاف قلم كل .. كان معايا تصريح من اليهود انه
+
+490
+00:47:21,910 --> 00:47:26,730
+تصريح يعني ك .. بجيت مسئول مستشفى ناصر فبجيت اطلع
+
+491
+00:47:26,730 --> 00:47:31,950
+قلم تمريضى بدنا اشتغله واروح الناس اللى خلصوا دوام
+
+492
+00:47:33,310 --> 00:47:37,530
+فكنا نلف و كنا نيجي في شوارع اليهود ضاربين فيها
+
+493
+00:47:37,530 --> 00:47:41,950
+غاز ساد جيه مابجيناش نستجر نجرب عليها ننخنج
+
+494
+00:47:41,950 --> 00:47:47,290
+فالمناطق اللي فيها تلوث في الهواء و فيها غازات
+
+495
+00:47:47,290 --> 00:47:51,130
+سامة و فيها اول اكسيد كربون او تاني اكسيد كربون
+
+496
+00:47:51,130 --> 00:47:55,850
+ماقدرش اخش فيها فكل هذه الشغلات بدي انا انجل
+
+497
+00:47:55,850 --> 00:48:01,910
+المصعف او المصاب عشان استطيع اجدمله اسعاف ماتعبش
+
+498
+00:48:01,910 --> 00:48:06,910
+انا وياهالمناطق اللي فيها غازات مش فيها اكسوجين
+
+499
+00:48:06,910 --> 00:48:17,070
+كفاية لاللي هي تنفس طب
+
+500
+00:48:17,070 --> 00:48:21,090
+انكب من هنا نبعد المصاب في عدة طرق لإبعاد المصاب
+
+501
+00:48:21,090 --> 00:48:29,710
+في عدة احتمالات الاحتمال الأول ان انا اكون وحدي
+
+502
+00:48:29,710 --> 00:48:32,070
+المصير
+
+503
+00:48:33,920 --> 00:48:39,840
+فقط مش في احتمال هيك اجي على منطقة فيها مشكلة و
+
+504
+00:48:39,840 --> 00:48:44,180
+مافيش حد يسعف الا انا و ألمساه بصير ولا بصيرش
+
+505
+00:48:44,180 --> 00:48:54,480
+الاحتمال الثاني ان انا المسعف مع شيء يعني في معايا
+
+506
+00:48:54,480 --> 00:49:00,180
+بطنية في معايا حرام في معايا كرسي في معايا اي حاجة
+
+507
+00:49:00,180 --> 00:49:10,430
+مظبوط ممكن استفيد منهااحتمال الثالث مصعف مع
+
+508
+00:49:10,430 --> 00:49:19,310
+مساعد او مصعف اخر يعني سنة كده ايش اتنين مقنعش اشي
+
+509
+00:49:19,310 --> 00:49:28,450
+احنا بني ادمين انسانين مصعف مع مساعد بدون شيء
+
+510
+00:49:28,450 --> 00:49:32,810
+بصيروا ولا بصيروا احنا اتنين جايين و في عندنا مصاب
+
+511
+00:49:32,810 --> 00:49:43,600
+و بدنا ننجلهاحتمال بعده مصعفين معشق ايش
+
+512
+00:49:43,600 --> 00:49:53,640
+يعني معشق مع كرسي مع بطني حرام لوح خشب اي حاجة
+
+513
+00:49:53,640 --> 00:50:00,600
+الاحتمال بعده ايش اكتر من
+
+514
+00:50:00,600 --> 00:50:03,400
+مصعفين
+
+515
+00:50:06,030 --> 00:50:15,430
+بدون شيء بعدين أكتر من مصرفين مع شيء في احتمالات
+
+516
+00:50:15,430 --> 00:50:27,130
+غير هيك فكروا في احتمالات غير هيك في في احتمالات
+
+517
+00:50:27,130 --> 00:50:34,930
+غير هيك تمام احتمال انه انا مصرف فقط مافيش معايا
+
+518
+00:50:34,930 --> 00:50:41,080
+ولا حاجةطب أنا بدي أسعر مين؟ بدي أسعر الإنسان،
+
+519
+00:50:41,080 --> 00:50:46,540
+الإنسان هذا أعمار، ممكن يكون طفل صغير، صح ولا لأ؟
+
+520
+00:50:46,540 --> 00:50:50,940
+طفل صغير فيش عنده صور بحمله بين إيديه و بمشي فيه،
+
+521
+00:50:50,940 --> 00:50:56,880
+صورين؟ بحمله بين إيديه و بمشي فيه، أحيانا صرايا
+
+522
+00:50:56,880 --> 00:51:05,630
+أنا يعني أذكر قصةانه كان في شباب واقفين من أيام
+
+523
+00:51:05,630 --> 00:51:12,410
+المطاردين زمان مسلحين وكان اليهود بعيدين عنهم يعني
+
+524
+00:51:12,410 --> 00:51:16,250
+مثل هان وقولوا بوابة الجامعة او الكراج تبع
+
+525
+00:51:16,250 --> 00:51:22,630
+السيارات اللى تحت فواحد قال يا شباب اليهود واحد
+
+526
+00:51:22,630 --> 00:51:27,310
+قال يا شباب اليهود عارفين ايش صعب؟ كانوا تلافى
+
+527
+00:51:28,360 --> 00:51:32,320
+اثنين شردوا والتالى ترتمى على الأرض ولا جوم مش
+
+528
+00:51:32,320 --> 00:51:39,640
+قادر اتجرطل زى ما بقوله بالبلد دى ماتخوف وقف تخشى
+
+529
+00:51:39,640 --> 00:51:45,900
+بخوف طب أنا ده كيف انا بيدنقله؟ كل بساطة لو مسكتيه
+
+530
+00:51:45,900 --> 00:51:52,460
+من يده بقى مظبوط ولا لا؟ ايش بده؟ ند فى الدعم نفسى
+
+531
+00:51:52,460 --> 00:51:57,140
+اخدين بالكوا كيف ففعلا اجوا حملوا واحد او مسكوا
+
+532
+00:51:57,140 --> 00:52:01,630
+واحدوشد طب انت يا زلم اليهود على بعد كذا و فاخر
+
+533
+00:52:01,630 --> 00:52:07,530
+الدنيا فكيف لو واجهوكم واجهة فسبحان الله العظيم
+
+534
+00:52:07,530 --> 00:52:11,910
+فلما يكون مسعف فقط بعض الناس بده بس تي مسكي منيد و
+
+535
+00:52:11,910 --> 00:52:20,410
+بمشي طفل صغير بهم بعض الناس بدك بس تدعميه نفسيالذا
+
+536
+00:52:20,410 --> 00:52:23,650
+انا مابقوم فش فيكش انت كويس مابقوم ساعدني بدل ما
+
+537
+00:52:23,650 --> 00:52:28,450
+انت في عندنا عشر و خمستاشر إصابة فيعني الجثة
+
+538
+00:52:28,450 --> 00:52:37,890
+بتتوقف على لباقة المصعف و وضع المصاب بكل بساطة
+
+539
+00:52:37,890 --> 00:52:43,130
+ممكن يعني و عمر المصاب كمان ممكن أحمله ممكن أمشي
+
+540
+00:52:43,130 --> 00:52:49,330
+ممكن زي انتوا شايفيني ممكن هنا في عدة صور كيف ممكن
+
+541
+00:52:49,330 --> 00:52:57,220
+واحدطلعوا صورة اللى فى صفحه 112 شايفينه؟
+
+542
+00:52:57,220 --> 00:53:02,240
+شايفين صورة 112؟ اى واحد صغير حامله بين اديه
+
+543
+00:53:02,240 --> 00:53:06,340
+الواحد التانى لجى لجى وماشي هو إياه حط يده على
+
+544
+00:53:06,340 --> 00:53:11,880
+كتفه وماشي هو إياه الصفحه اللى بعدها حامله على
+
+545
+00:53:11,880 --> 00:53:15,760
+ظهره حامله على ظهره كيف ممكن انا أحمل واحد على
+
+546
+00:53:15,760 --> 00:53:22,730
+ظهري بوقفه وبعدين بحط ظهري فى ظهرهفإذا كنت واحد
+
+547
+00:53:22,730 --> 00:53:31,530
+لحالي ممكن أعمل هيك شغلات يا عجبتك الفكرة؟ يعني
+
+548
+00:53:31,530 --> 00:53:35,910
+أنتوا ملاحظين يعني ال .. ال .. الإسعاف الأولي بده
+
+549
+00:53:35,910 --> 00:53:40,290
+ديارة مش بس الإسعاف الأول حياة كلها بده ديارة ولا
+
+550
+00:53:40,290 --> 00:53:46,190
+لأ بدك تكون شاطرة و لبقة و مديرة و ماتجفيش عند حد
+
+551
+00:53:46,190 --> 00:53:50,890
+معين تقولي خلصت الدنيافممكن جدا زى ما قلنا كمان
+
+552
+00:53:50,890 --> 00:53:57,590
+ممكن جدا احمله احط ايدي بالرجله و الايد التانى
+
+553
+00:53:57,590 --> 00:54:02,270
+تمسك و احطه ع رجلي اذا كان خفيف شوية و امشي فيه
+
+554
+00:54:02,270 --> 00:54:08,290
+اودى وين بدى فكل هذه شغلات اذا كان مسعف بدون اشي
+
+555
+00:54:08,290 --> 00:54:14,150
+من الحاجات اللى ممكن يعملها المسعف بدون اشي لو
+
+556
+00:54:14,150 --> 00:54:18,150
+واحد نايم على الأرضواحد نايم على الأرض بقدر أنقله
+
+557
+00:54:18,150 --> 00:54:24,430
+ولا بقدرش؟ لو جيت أنا مافيش فيه قصور طبعا، مسكته
+
+558
+00:54:24,430 --> 00:54:29,930
+وجريته من رجليها، بنجره ولا بنجرش؟ سهل، حتى لو كان
+
+559
+00:54:29,930 --> 00:54:34,270
+وزنه أضجر من وزني بكتير، واشده من رجليها، ممكن
+
+560
+00:54:34,270 --> 00:54:39,750
+انشده، لكن إيش الفكرة من شده من رجليها؟ الفكرة شده
+
+561
+00:54:39,750 --> 00:54:44,090
+من رجليها ممكن تسلخ دهرو إذا الأرض فيها حصو ورمل
+
+562
+00:54:44,090 --> 00:54:44,790
+وإش زي هي
+
+563
+00:54:48,770 --> 00:54:55,830
+نعم كأنه انحرج الجلد اتسلخ فبدي احط حاجة بين دهرو
+
+564
+00:54:55,830 --> 00:55:01,310
+و بين ايش القرض اللي انا بشد فيها مافيش حاجة بدي
+
+565
+00:55:01,310 --> 00:55:06,010
+احاول اشد ملابس و اربطها كويس اذا في اجشاط في
+
+566
+00:55:06,010 --> 00:55:13,370
+بنطلون بالذرر نيح بحيث انه مايحكش جسمه و يعمل جرح
+
+567
+00:55:13,370 --> 00:55:19,800
+خدشي يدخل التهاباتممكن بلاش من الرجلين يا ستي ممكن
+
+568
+00:55:19,800 --> 00:55:24,520
+اجي من تحت اباطه ولا لأ لو مسجناها من تحت القبط
+
+569
+00:55:24,520 --> 00:55:28,500
+اللي حطيت إيدي تحت اباطه التانية وسعبت هذه الفكرة
+
+570
+00:55:28,500 --> 00:55:33,820
+أسهل من الرجلين ليش؟ لأنه كل ما شديت الملابس بدل
+
+571
+00:55:33,820 --> 00:55:38,900
+ما تطلع فوق بتنزل تحت فلما تنزلش بتحمي بتحمي الظهر
+
+572
+00:55:38,900 --> 00:55:45,420
+بتسلخش طبعا ممكن تتمزع الملابس مع السلام مش مشكلة
+
+573
+00:55:45,420 --> 00:55:51,440
+المهم إيشإذا ضل البناء آدم بيكون احنا حققنا الهدف
+
+574
+00:55:51,440 --> 00:55:56,440
+الأمثل فيعني لو كان مسعف واحد وماعوش ولا إشي بقدر
+
+575
+00:55:56,440 --> 00:56:00,680
+أعمل كل هالشغلات زي ما أنتوا شايفين شغلات مش وسيطة
+
+576
+00:56:00,680 --> 00:56:08,920
+ونجلت المسعف بسهولة بدون أي مشاكل الآن
+
+577
+00:56:08,920 --> 00:56:15,000
+ممكن يكون عندي مسعف ومعاه إشي إيش ممكن يكون مع
+
+578
+00:56:15,000 --> 00:56:15,620
+المسعف؟
+
+579
+00:56:19,220 --> 00:56:27,200
+لو حطينا على لوحة خشب بنجر لو عنده كرسي لو عنده
+
+580
+00:56:27,200 --> 00:56:33,520
+كرسي و جلدت البني أدم على الكرسي و جيت تعجته زي
+
+581
+00:56:33,520 --> 00:56:40,160
+هيك و سحبته بسحبه ولا بسحبوش بسحبه و بيصير وضع
+
+582
+00:56:40,160 --> 00:56:47,960
+كويس طب لو عندي انا شرشف او بطنية و نيمت عليها و
+
+583
+00:56:47,960 --> 00:56:53,590
+شحطتبودي على مسافة بعيدة جدا، ماخدين بالكوا كيف؟
+
+584
+00:56:53,590 --> 00:56:58,410
+فبكل بساطة يعني لو كان عندى مسعف واحد ومعاه اشي
+
+585
+00:56:58,410 --> 00:57:02,530
+يعني انتوا بتكونوا تعرفوا بالظبط انه انت مديرة
+
+586
+00:57:02,530 --> 00:57:08,270
+الموقف، انت اللي الخطيف رجبتكانت اللي الله راح
+
+587
+00:57:08,270 --> 00:57:13,750
+حاسبك إذا قصرتي في إسعاب بني آدم و أنت بتقدر
+
+588
+00:57:13,750 --> 00:57:17,190
+تعمليها مش بس إسعاب بني آدم أي حاجة بتقدر تعمليها
+
+589
+00:57:17,190 --> 00:57:21,790
+خير تنقذ فيها شيء معين و تقصري ممكن جدا تتحسب
+
+590
+00:57:21,790 --> 00:57:26,350
+عليها أمام الله سبحانه وتعالى فانت واتقوا الله و
+
+591
+00:57:26,350 --> 00:57:30,310
+يعلمكم الله قول يا رب و صدقي ما بتعرف من وين بيجيك
+
+592
+00:57:30,310 --> 00:57:34,940
+الفرج بس وكليها لمينلله سبحانه وتعالى و الفرج
+
+593
+00:57:34,940 --> 00:57:38,960
+بيجيكي يعني بطريقة كويسة فلما يكون عندى مصحف واحد
+
+594
+00:57:38,960 --> 00:57:45,120
+وماعوش ولا اشي زى ما انتوا شايفين ممكن جدا انه
+
+595
+00:57:45,120 --> 00:57:50,640
+ينقل المصاب من منطقة الخطر الى منطقة الأمان الحين
+
+596
+00:57:50,640 --> 00:57:56,560
+المرة الجاية بنحكي عن مصحف وماعاه اشي ماشي الحال
+
+597
+00:57:56,560 --> 00:58:04,020
+بمشي الحال اللى الحين نحكيهنا أسئلة أسئلة في حالة
+
+598
+00:58:04,020 --> 00:58:08,880
+مثلا لو أجت مية نار على الرجل مين تقولها؟ حكيناها
+
+599
+00:58:08,880 --> 00:58:12,340
+هذا، مين يا بنات تقول؟ لو أجت مية نار على الرجل؟
+
+600
+00:58:12,340 --> 00:58:17,440
+نظلنا نغسل فيها المي على طول وبنعادلها بعد ما نخلص
+
+601
+00:58:17,440 --> 00:58:22,360
+غسيل بمادة قلوية كربونة، كربونة الطبيخ اللي في
+
+602
+00:58:22,360 --> 00:58:26,960
+الدار، تحط معلقة كربونةعلى كباية ميوبة حركية
+
+603
+00:58:26,960 --> 00:58:31,800
+وبدريها على المنطقة لإن الكربون قلوي والميط النار
+
+604
+00:58:31,800 --> 00:58:37,980
+حامض ماشي؟ بتصير ان شاء الله كويسة أي سؤال تاني؟
+
diff --git a/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/MZMxT0v6xi8_raw.json b/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/MZMxT0v6xi8_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..9be17ce1db00ad116096312b10ccfc418abe5793
--- /dev/null
+++ b/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/MZMxT0v6xi8_raw.json
@@ -0,0 +1 @@
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"end": 3497.44, "word": " نخلص", "probability": 0.989501953125}, {"start": 3497.44, "end": 3497.9, "word": " غسيل", "probability": 0.9441731770833334}, {"start": 3497.9, "end": 3499.64, "word": " بمادة", "probability": 0.80859375}, {"start": 3499.64, "end": 3500.16, "word": " قلوية", "probability": 0.924072265625}, {"start": 3500.16, "end": 3500.88, "word": " كربونة،", "probability": 0.6912109375}, {"start": 3500.88, "end": 3501.7, "word": " كربونة", "probability": 0.9534912109375}, {"start": 3501.7, "end": 3502.1, "word": " الطبيخ", "probability": 0.98291015625}, {"start": 3502.1, "end": 3502.26, "word": " اللي", "probability": 0.88330078125}, {"start": 3502.26, "end": 3502.36, "word": " في", "probability": 0.9677734375}, {"start": 3502.36, "end": 3503.68, "word": " الدار،", "probability": 0.8536783854166666}, {"start": 3503.68, "end": 3503.96, "word": " تحط", "probability": 0.7285563151041666}, {"start": 3503.96, "end": 3504.36, "word": " معلقة", "probability": 0.645751953125}, {"start": 3504.36, "end": 3505.04, "word": " كربونة", "probability": 0.95654296875}], "temperature": 1.0}, {"id": 131, "seek": 351798, "start": 3505.46, "end": 3517.98, "text": "على كباية ميوبة حركية وبدريها على المنطقة لإن الكربون قلوي والميط النار حامض ماشي؟ بتصير ان شاء الله كويسة أي سؤال تاني؟", "tokens": [3615, 23942, 9122, 3555, 995, 10632, 3714, 1829, 37746, 3660, 11331, 31747, 10632, 46599, 3215, 16572, 11296, 15844, 9673, 1863, 9566, 28671, 5296, 28814, 1863, 33251, 25513, 11536, 12174, 1211, 45865, 16070, 2304, 1829, 9566, 2423, 8315, 2288, 11331, 10943, 11242, 3714, 33599, 1829, 22807, 39894, 9381, 13546, 16472, 13412, 16606, 21984, 9122, 45865, 3794, 3660, 36632, 8608, 33604, 6027, 6055, 7649, 1829, 22807], "avg_logprob": -0.2899038461538462, "compression_ratio": 1.6102941176470589, "no_speech_prob": 0.00025534629821777344, "words": [{"start": 3505.46, "end": 3505.76, "word": "على", "probability": 0.343505859375}, {"start": 3505.76, "end": 3506.18, "word": " كباية", "probability": 0.802490234375}, {"start": 3506.18, "end": 3506.62, "word": " ميوبة", "probability": 0.865478515625}, {"start": 3506.62, "end": 3506.96, "word": " حركية", "probability": 0.9348958333333334}, {"start": 3506.96, "end": 3507.48, "word": " وبدريها", "probability": 0.66766357421875}, {"start": 3507.48, "end": 3507.66, "word": " على", "probability": 0.8193359375}, {"start": 3507.66, "end": 3508.3, "word": " المنطقة", "probability": 0.982177734375}, {"start": 3508.3, "end": 3509.0, "word": " لإن", "probability": 0.7312825520833334}, {"start": 3509.0, "end": 3509.68, "word": " الكربون", "probability": 0.8255208333333334}, {"start": 3509.68, "end": 3510.18, "word": " قلوي", "probability": 0.8193359375}, {"start": 3510.18, "end": 3511.34, "word": " والميط", "probability": 0.5006103515625}, {"start": 3511.34, "end": 3511.8, "word": " النار", "probability": 0.7881673177083334}, {"start": 3511.8, "end": 3512.6, "word": " حامض", "probability": 0.9881184895833334}, {"start": 3512.6, "end": 3513.34, "word": " ماشي؟", "probability": 0.68487548828125}, {"start": 3513.34, "end": 3514.0, "word": " بتصير", "probability": 0.736328125}, {"start": 3514.0, "end": 3514.48, "word": " ان", "probability": 0.79296875}, {"start": 3514.48, "end": 3514.68, "word": " شاء", "probability": 0.984375}, {"start": 3514.68, "end": 3514.92, "word": " الله", "probability": 0.96044921875}, {"start": 3514.92, "end": 3516.26, "word": " كويسة", "probability": 0.9830322265625}, {"start": 3516.26, "end": 3516.76, "word": " أي", "probability": 0.237548828125}, {"start": 3516.76, "end": 3517.12, "word": " سؤال", "probability": 0.98828125}, {"start": 3517.12, "end": 3517.98, "word": " تاني؟", "probability": 0.9228515625}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 3519.1235, "duration_after_vad": 3371.2543749999872}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/Owc1NKWveag_raw.srt b/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/Owc1NKWveag_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..fe662d1cab25b803ceefd070368a719450cc4e82
--- /dev/null
+++ b/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/Owc1NKWveag_raw.srt
@@ -0,0 +1,3192 @@
+1
+00:00:20,670 --> 00:00:25,470
+اليوم ان شاء الله نتكلم عن موضوع الصدمة والغيبومة
+
+2
+00:00:25,470 --> 00:00:30,470
+في شيء من اللي شرحناه قبل عايزين تسأله فيه في اي
+
+3
+00:00:30,470 --> 00:00:39,070
+سؤال في اللي فات كله واضح ان شاء الله نبدأ
+
+4
+00:00:39,070 --> 00:00:44,090
+في موضوع اسمه الصدمة حد عنده فكرة ايش يعني الصدمة
+
+5
+00:00:45,580 --> 00:00:50,220
+ماذا تعني الصدمة؟ مال فلان والله يا أخوي مظلومة،
+
+6
+00:00:50,220 --> 00:00:56,740
+إيش يعني مظلومة؟ ماذا تعني لكون كلمة صدمة؟ الصدمة
+
+7
+00:00:56,740 --> 00:01:03,940
+شيء إليه علاقة في الدم والأكسوجين اللي بيصل
+
+8
+00:01:03,940 --> 00:01:10,740
+للأجهزة الحيوية لما يخس الأكسوجين والدم اللي بيصل
+
+9
+00:01:10,740 --> 00:01:18,600
+خاصة للمخبصير حاجة اسمها صدمة كمان لما يصل دم إلى
+
+10
+00:01:18,600 --> 00:01:28,260
+المخ مش نقي او دم لا يستفيد منه المخ بيصير حاجة
+
+11
+00:01:28,260 --> 00:01:32,540
+اسمها صدمة فهي عبارة عن نقص تروية الأنسجة يعني
+
+12
+00:01:32,540 --> 00:01:39,140
+الأنسجة لا ترتوي كفاية بالدم اللي بيصلها بيصل دم
+
+13
+00:01:39,140 --> 00:01:47,610
+بسبيستفيدش منه الجسم او مش كفاية الناتج عن عدم
+
+14
+00:01:47,610 --> 00:01:50,990
+التوازن بين حاجات خلايا الجسم و ما يصلها منها ال
+
+15
+00:01:50,990 --> 00:01:55,210
+oxygen كيف بيصل ال oxygen للجسم؟ عن طريق الدورة
+
+16
+00:01:55,210 --> 00:02:01,190
+الدموية الدم بيحمل ال oxygen و بيحمل التغذية لباقي
+
+17
+00:02:01,190 --> 00:02:07,370
+الخلايا فبتصير فيه ما يسمى اللي هي الغذاء و
+
+18
+00:02:07,370 --> 00:02:08,330
+التروية
+
+19
+00:02:10,390 --> 00:02:16,170
+مما يؤدي إلى قصور في الوظائف الحيوية خاصة على
+
+20
+00:02:16,170 --> 00:02:21,030
+مستوى اللي هو الحياة حيث يستخدم ال oxygen لتوليد
+
+21
+00:02:21,030 --> 00:02:26,990
+مصدر الطاقة في الجسم بدون ال oxygen نستفيدش من
+
+22
+00:02:26,990 --> 00:02:33,110
+الأكل ال oxygen هو الوقودهو اللى بيعملنا الطاقة هو
+
+23
+00:02:33,110 --> 00:02:36,290
+اللى بيعملنا النمو هو اللى بيعملنا كل الأشياء اللى
+
+24
+00:02:36,290 --> 00:02:41,290
+احنا ويصبح هذا الخلل غير قابل للاصلاح اذا تأخر
+
+25
+00:02:41,290 --> 00:02:46,750
+العلاج ايش المفهوم المعاكس المفهوم المعاكس انه
+
+26
+00:02:46,750 --> 00:02:53,410
+ممكن يعالج اذا اتينا للعلاج في الوقت المبكر او في
+
+27
+00:02:53,410 --> 00:03:00,350
+الوقت الصحيح وإلا اذا تأخرنا ممكن يسوي مشاكلما
+
+28
+00:03:00,350 --> 00:03:09,510
+يكون سهل ألاجة في
+
+29
+00:03:09,510 --> 00:03:16,070
+كمان تعريف تاني للصدمة فشل الجهاز الدوري اللي
+
+30
+00:03:16,070 --> 00:03:20,730
+عبارة عن القلب والأوعية الدموية في تزويد خلايا
+
+31
+00:03:20,730 --> 00:03:25,350
+الجسم بكمية كافية من الدم المحمل بالأكسجين عشان
+
+32
+00:03:25,350 --> 00:03:35,000
+يروي الأنسجال الحيويةبصل أكسجين غير كافي للأجهزة
+
+33
+00:03:35,000 --> 00:03:39,720
+الحيوية فبصير عند البني آدم صدمة فشل الجهاز الدوري
+
+34
+00:03:39,720 --> 00:03:44,160
+اللي هو القلب والأوعي الدموية في تزويد خلايا الجسم
+
+35
+00:03:44,160 --> 00:03:51,380
+الحيوية ماشي بكمية كافية من الدم المحمل بالأكسجين
+
+36
+00:03:51,380 --> 00:03:56,760
+بصليش أكسجين كفاية لإرواع الأنسجة الحيوية
+
+37
+00:03:59,710 --> 00:04:05,130
+طبعا هذه الصدمة إيش بسببها الصدمة
+
+38
+00:04:05,130 --> 00:04:08,730
+هذه إيه لها عدة أسباب طبعا هنا كاتب هو أسباب
+
+39
+00:04:08,730 --> 00:04:13,490
+الصدمة أسباب الصدمة بمعنى أنواع الصدمة إذا قلنا
+
+40
+00:04:13,490 --> 00:04:20,270
+أسباب الصدمة أو أنواع الصدمة بتحمل نفس المعنى لابد
+
+41
+00:04:20,270 --> 00:04:26,830
+من تحديد سبب للصدمة الإصابة النزيف المرض غالبا
+
+42
+00:04:26,830 --> 00:04:34,710
+أسباب الصدمةموجودة في أحد الثلاثة إما إصابة وإما
+
+43
+00:04:34,710 --> 00:04:43,970
+نزيف وإما مرض في صدمة ناتجة عن نقص حجم الدم تعالوا
+
+44
+00:04:43,970 --> 00:04:51,910
+هنا تعالوا هنا صدمة ناتجة عن نقص حجم الدم ممكن
+
+45
+00:04:51,910 --> 00:04:53,970
+تقولوا إيش يعني نقص حجم الدم
+
+46
+00:05:00,760 --> 00:05:05,180
+ممكن حجم الدم يصير بحاجتين الحاجة الأولى نظيف
+
+47
+00:05:05,180 --> 00:05:12,580
+الحاجة التانية مش شرط المرض يعني انا بحكيش عن قوة
+
+48
+00:05:12,580 --> 00:05:18,640
+الدم يعني بتروح بتجيس الدم بتلاقي اتناش اربتاش
+
+49
+00:05:18,640 --> 00:05:27,160
+عشرة بكون حجمه طبيعي حجم الدم يعني كمية الدم ماشي
+
+50
+00:05:27,160 --> 00:05:31,800
+احنا عندنا في الجسم سبعة فالمية من جسمنادم هذا
+
+51
+00:05:31,800 --> 00:05:36,660
+الدم ممكن يكون hemoglobin 5 وحجمه 7% وممكن يكون
+
+52
+00:05:36,660 --> 00:05:43,080
+hemoglobin 15 وبرضه حجمه 7% احنا بنحكي عن نقص حجم
+
+53
+00:05:43,080 --> 00:05:50,440
+الدم نقص حجم الدم ممكن يصير من النزيل قال لو جيبنا
+
+54
+00:05:50,440 --> 00:05:58,640
+كاسة ماء ملناها ميه وسكر حطينا في الميهمثلا عشرة
+
+55
+00:05:58,640 --> 00:06:03,260
+في المية سكر وتسعين في المية ميه وحركناها بالسير
+
+56
+00:06:03,260 --> 00:06:09,140
+الميه السكر الى الميه واحد الى عشرة مظبوط الان لو
+
+57
+00:06:09,140 --> 00:06:13,160
+مسكن الكباية بعد ما دوبنا السكر بشكل طبيعى وكبنا
+
+58
+00:06:13,160 --> 00:06:17,920
+منها ايش بيصير في حجم السائل حجم الميه بتقل فنقص
+
+59
+00:06:17,920 --> 00:06:25,740
+حجم السائل نقص حجم الدم لو واحد نزفو الدم خرج إلى
+
+60
+00:06:25,740 --> 00:06:30,180
+الخارج بصير نقص لإيش؟ لحجم الدم طب لما في رمضان
+
+61
+00:06:30,180 --> 00:06:36,660
+بنعمل قطر بتعمل قطر زينا و لا لأ؟ كيف بتعملوه؟ و
+
+62
+00:06:36,660 --> 00:06:44,460
+لمون ماشي كيف بتسووا؟
+
+63
+00:06:44,460 --> 00:06:47,840
+بنغلي
+
+64
+00:06:47,840 --> 00:06:54,760
+السكر و الميه و بنحط اللمونو بنغليه ماشي ايش بيصير
+
+65
+00:06:54,760 --> 00:07:01,440
+في حجم الميه؟ و ايش بيصير في الجطر في السكر؟ بثقل
+
+66
+00:07:01,440 --> 00:07:12,800
+لكن حجم الميه خس يبقى حجم السائل خس السكر خس موجود
+
+67
+00:07:12,800 --> 00:07:21,540
+بس موجود ولا gram ولا milli سكرخس بمعنى انه كمية
+
+68
+00:07:21,540 --> 00:07:26,820
+السائل خلصت لكن كمية محتوى السائل ضايلة هيك بالدم
+
+69
+00:07:26,820 --> 00:07:31,560
+بيصير كمية محتوى الدم كرات الدم الحمرا و كرات الدم
+
+70
+00:07:31,560 --> 00:07:36,040
+البيضا و البلازمة البلازمة بتخس كرات الدم الحمرا و
+
+71
+00:07:36,040 --> 00:07:40,900
+كرات الدم البيضا موجودة لكن سوائل الدم طلعت وقت
+
+72
+00:07:40,900 --> 00:07:47,310
+إيش هذا بيصير في الجفاف وقت إيش بيصير الجفافواحد
+
+73
+00:07:47,310 --> 00:07:50,430
+طفل بتوديه على مستشفى لأطفال كان قالك الطفل عنده
+
+74
+00:07:50,430 --> 00:07:54,810
+جفاف ايش يعني جفاف الولد بيسهل و بنتج له تلت أيام
+
+75
+00:07:54,810 --> 00:08:00,630
+بيسهل و بنتج بمعنى كل ما ياخد حاجة بيستفيدش منها
+
+76
+00:08:00,630 --> 00:08:05,370
+جسمه و في المقابل بيطلع من جسمه اشي بيطلع مثلا
+
+77
+00:08:05,370 --> 00:08:10,010
+بول، بيطلع عبراز، بيطلع كذا فجسمه بيخس فبصير الجسم
+
+78
+00:08:10,010 --> 00:08:14,270
+ياخد من ذاته تطلع إلى الولد بيصير زي العوض و
+
+79
+00:08:14,270 --> 00:08:19,720
+العظمةفيش الجلد واللحم زي ما بقوله ناشف شوفت عمرك
+
+80
+00:08:19,720 --> 00:08:26,260
+ولد عنده جفاف صغير ناشف مظبوط هذا اسمه جفاف لو
+
+81
+00:08:26,260 --> 00:08:32,500
+جيتي فحصتيله الدم بتلاقي دم مجدير لا بتلاقيه عالي
+
+82
+00:08:32,500 --> 00:08:36,940
+جدا بتلاقيه عالي جدا ليش؟ ازاي ما بنعمل اليطار
+
+83
+00:08:36,940 --> 00:08:42,240
+اتركز الخلايا في مسافة قليلة من او في حجم قليل من
+
+84
+00:08:42,240 --> 00:08:49,940
+الدم فصارت كبيرةفال .. نقص حجم الدم ممكن يصير ..
+
+85
+00:08:49,940 --> 00:08:53,600
+في عند دم لكن حجمه غير كافي فبصارش كفاية لوين
+
+86
+00:08:53,600 --> 00:08:59,800
+للأنسجة الحيوية فالقيق والاسهال الجفاف عدم الأكل
+
+87
+00:08:59,800 --> 00:09:03,460
+اللي بضربه عن الطعام الناس اللي بضربه بتحس في
+
+88
+00:09:03,460 --> 00:09:08,660
+السجن بقعدوا كده يوم بوكلوش ولا بشربوا بيصيروا
+
+89
+00:09:08,660 --> 00:09:14,930
+يحملوهم حملوا مش قادرين يتحركوا بيستوواليش؟ لأنه
+
+90
+00:09:14,930 --> 00:09:21,850
+خس حجم الدم ففي صدمة ناتجة عن نقص حجم الدم لما خس
+
+91
+00:09:21,850 --> 00:09:26,190
+حجم الدم بيصير يصل المخ كفاية فبصير المخ مايصلوش
+
+92
+00:09:26,190 --> 00:09:33,530
+كفاية النوع
+
+93
+00:09:33,530 --> 00:09:38,230
+التاني صدمة
+
+94
+00:09:38,230 --> 00:09:46,890
+اللي لها علاقة بالأوعية الدمويةإيش يعني؟ الأوعية
+
+95
+00:09:46,890 --> 00:09:51,970
+الدموية هاد في الشيوب بتتوسع فبصير
+
+96
+00:09:51,970 --> 00:09:56,070
+.. احنا قلنا في الوضع الطبيعي لما يصير صدمة نقص
+
+97
+00:09:56,070 --> 00:09:59,510
+حجم الدم الجسم بيفرز مادة ال adrenaline بتدج
+
+98
+00:09:59,510 --> 00:10:03,310
+الأوعية الدموية فبصير الدم وينروح على الخلايا
+
+99
+00:10:03,310 --> 00:10:09,910
+الحية على القلب و الرأتين و المخ الحين لو اتوسعت
+
+100
+00:10:09,910 --> 00:10:16,920
+الأوعية الدموية إيش هيصير؟هيخس الدم عن المخ ولا لأ
+
+101
+00:10:16,920 --> 00:10:24,220
+فصار نقص وصول الدم الى المخ صدمة متصلة بالقلب كله
+
+102
+00:10:24,220 --> 00:10:31,620
+هدف حجم الدم الجلب سياد تعين مريض او عليد بمعنى
+
+103
+00:10:31,620 --> 00:10:37,400
+ايش بيشتغل القلب مضخة للجسم المضخة هذه ايش بتساوي
+
+104
+00:10:37,400 --> 00:10:43,970
+توصل الدم مع ال oxygen الى كل خلية في الجسمالحين
+
+105
+00:10:43,970 --> 00:10:50,230
+وين ما أنخز إبرة بطيح دم مظبوط؟ إيش معناته؟ معناته
+
+106
+00:10:50,230 --> 00:10:57,450
+الدم يصل إلى كل جزئية من جزئيات الإنسان فلما القلب
+
+107
+00:10:57,450 --> 00:11:06,610
+يكون فيه مشكلة عضلة القلب مريضة فيه ضعف في القلب
+
+108
+00:11:06,610 --> 00:11:12,500
+فيه جلطة في القلب فيه هبوط في القلبفي أي حاجة
+
+109
+00:11:12,500 --> 00:11:17,040
+بيصير القلب غير قادر .. في دم لكن فيش motor فيش
+
+110
+00:11:17,040 --> 00:11:23,140
+جهاز يرفع الدم يصل لكل الأماكن اللي لازم يصل لها
+
+111
+00:11:23,140 --> 00:11:27,040
+فبخش الإنسان بصدمة .. ناحظي انت كل الشغل اللي لها
+
+112
+00:11:27,040 --> 00:11:33,140
+علاقة بإيش؟ بوصول ال oxygen إلى الإنسان و الأجهزة
+
+113
+00:11:33,140 --> 00:11:43,170
+الحيوية في صدمة اسمها الصدمة التسمميةمرضت عنكم
+
+114
+00:11:43,170 --> 00:11:48,950
+صدمة تسمومية إيش عن صدمة تسمومية؟ يعني البني آدم
+
+115
+00:11:48,950 --> 00:11:57,110
+دمه فيه أخد سم السم ممكن ييجي من الإنسان ذاته و
+
+116
+00:11:57,110 --> 00:12:03,910
+ممكن ييجي من الخارج كيف بيجي السم من الإنسان ذاته؟
+
+117
+00:12:03,910 --> 00:12:10,280
+هذا من الخارج التهيبات في جسمهالتهابات في جسمه،
+
+118
+00:12:10,280 --> 00:12:14,600
+جسمه صار فيه التهابات و بدأ يفرز الميكروب يفرز
+
+119
+00:12:14,600 --> 00:12:18,340
+المواد السامة فبصير تسمم، الدم اللي بيصل للمخ
+
+120
+00:12:18,340 --> 00:12:22,520
+عمركوش سمعته الطفل أخدوه عن المستشفى و قالوا عنده
+
+121
+00:12:22,520 --> 00:12:27,120
+تسمم في الدم تسمم
+
+122
+00:12:27,120 --> 00:12:33,020
+في الدم و عنده حرارة و عنده وضع تأبزي جرثومة في
+
+123
+00:12:33,020 --> 00:12:37,600
+الدم بكثير هذا اسمه التسمم في الدم التسمم في الدم
+
+124
+00:12:37,600 --> 00:12:42,620
+يعني أن الدم أصبح غير نقي يعني صار يصل إلى الجسم
+
+125
+00:12:42,620 --> 00:12:49,740
+للأجهزة الحيوية بكميات ولكن غير مفيدةفبساوي ما
+
+126
+00:12:49,740 --> 00:12:54,360
+يسمى بالصدمة هذه اسمها الصدمة التساممية من الذات
+
+127
+00:12:54,360 --> 00:12:58,840
+في صدمة تساممية زي ما قالت الأخت من ان اجيب دوة او
+
+128
+00:12:58,840 --> 00:13:04,160
+اجيب مادة سامة و اشربها المادة او ألذق بواسطة
+
+129
+00:13:04,160 --> 00:13:11,120
+صعبان بواسطة عقرب بواسطة شيء يعمللي تسامم التسامم
+
+130
+00:13:11,120 --> 00:13:16,120
+هذا بيدخل على الدم السام بيخرب الدم الدم بيصير
+
+131
+00:13:16,120 --> 00:13:21,450
+يعنيغير قابل الجسم للاستفادة منه فبموت الإنسان إذا
+
+132
+00:13:21,450 --> 00:13:24,990
+مالحقنهوش ماخدين بالك و كده فهذه الصدمة تسموها في
+
+133
+00:13:24,990 --> 00:13:30,110
+حاجة اسم الصدمة ايش الصدمة العصبية الصدمة العصبية
+
+134
+00:13:30,110 --> 00:13:36,290
+او النفسية يعني بتيجي مثلا صاحبيتك او يعني هذا كان
+
+135
+00:13:36,290 --> 00:13:43,710
+اليوم في كان عندنا شاب خاطب ووزع الكرتات يوم الأحد
+
+136
+00:13:43,710 --> 00:13:49,970
+بده يتجوزيوم اللي في الجمعة دخل عملية استشهادية أو
+
+137
+00:13:49,970 --> 00:13:56,590
+هي استشهد، مين أكتر الناس أغمى عليه وصدمه؟ أم
+
+138
+00:13:56,590 --> 00:14:07,610
+العروسة، صدم، نعم، أه، بنتها، وموزعة كارت الفرح،
+
+139
+00:14:07,610 --> 00:14:14,830
+وودوها لمستشفى وعلاج، لمانياش، صدمنا، عصبيفالبني
+
+140
+00:14:14,830 --> 00:14:21,430
+آدم الله سبحانه وتعالى يعني خلقنا كبشر وعلمنا انه
+
+141
+00:14:21,430 --> 00:14:24,730
+نرضى بقضاء الله عشان هيك النبي صلى الله عليه و سلم
+
+142
+00:14:24,730 --> 00:14:30,950
+بيقول عجبا لأمر المؤمن ان أمره كله كله بمعنى اللي
+
+143
+00:14:30,950 --> 00:14:35,790
+بيشوفه كويس و اللي مش كويس كله احنا بنشوف قطأه صح
+
+144
+00:14:35,790 --> 00:14:41,050
+لكن لو اطلعتم الغيب لاختارتم الواقع ان أمره كله له
+
+145
+00:14:41,050 --> 00:14:47,010
+ان أصابته سراءفكان خير الله وإن أصابته ضراء فكان
+
+146
+00:14:47,010 --> 00:14:51,010
+خير الله وليس ذلك إلا أن المؤمن أوقف مقال صلى الله
+
+147
+00:14:51,010 --> 00:14:56,610
+عليه وسلم فخشت المرأة يعني في مشكلة نفسية عصبية
+
+148
+00:14:56,610 --> 00:15:02,310
+وخشت في صدمة هذه اسمها الصدمة العصبية الصدمة
+
+149
+00:15:02,310 --> 00:15:07,350
+العصبية أخوات المجتمع ممكن تيجي من شدة الفرح وممكن
+
+150
+00:15:07,350 --> 00:15:11,870
+تيجي من سدة الحزن ماخدين بعتكم؟ من شدة الفرح برضه
+
+151
+00:15:11,870 --> 00:15:15,820
+ممكن واحد يعنيبقى هنا يوم من الأيام أنا و شاب و
+
+152
+00:15:15,820 --> 00:15:22,880
+خاله واقفين فأجى لخاله خبر انه انت انجحت في مصر
+
+153
+00:15:22,880 --> 00:15:26,840
+طبعا خالي بدرس انت انجحت و أخدت الأول ع الكلية
+
+154
+00:15:26,840 --> 00:15:33,260
+اللي و جأ بين أدينا و جأ من شدة الفرح من شدة الفرح
+
+155
+00:15:33,260 --> 00:15:37,020
+و جأ بين أدينا فبكل بساطة البني آدم يعني يجب أن
+
+156
+00:15:37,020 --> 00:15:41,780
+يكون متزن و إلا ميتزن بيخش في مشاكل كبيرة
+
+157
+00:15:44,890 --> 00:15:51,590
+ف من كمان الصدمات في حاجة اسمها الصدمة الكهربائية
+
+158
+00:15:51,590 --> 00:15:56,670
+ماشي ايش عن الكهربائية مين عمرها صبتها مين عمرها
+
+159
+00:15:56,670 --> 00:16:01,550
+صبتها الكهربا ماظننيش ماصبتكنش الكهربا يعني كل
+
+160
+00:16:01,550 --> 00:16:05,910
+صبتنا الكهربا الكهربا ايش بيساوي لما تصيب البني
+
+161
+00:16:05,910 --> 00:16:11,150
+آدم بتخيل
+
+162
+00:16:11,150 --> 00:16:16,670
+واحد مايحكمش نفسهمايحكمش كأنك انت صايباك مش زمان،
+
+163
+00:16:16,670 --> 00:16:22,590
+صح؟ ايش صار معاك؟ انا انا انا انا انا انا انا انا
+
+164
+00:16:22,590 --> 00:16:23,790
+انا انا انا انا انا انا انا انا انا انا انا انا
+
+165
+00:16:23,790 --> 00:16:24,550
+انا انا انا انا انا انا انا انا انا انا انا انا
+
+166
+00:16:24,550 --> 00:16:25,170
+انا انا انا انا انا انا انا انا انا انا انا انا
+
+167
+00:16:25,170 --> 00:16:27,370
+انا انا انا انا انا انا انا انا انا انا انا انا
+
+168
+00:16:27,370 --> 00:16:30,750
+انا انا انا انا انا انا انا انا انا انا انا انا
+
+169
+00:16:30,750 --> 00:16:30,750
+انا انا انا انا انا انا انا انا انا انا انا انا
+
+170
+00:16:30,750 --> 00:16:30,790
+انا انا انا انا انا انا انا انا انا انا انا انا
+
+171
+00:16:30,790 --> 00:16:39,590
+انا انا انا
+
+172
+00:16:39,590 --> 00:16:39,590
+انا
+
+173
+00:16:43,320 --> 00:16:47,620
+عارفين إيش بيصير بالظبط الكهربا؟ عشان تعرفوا كده
+
+174
+00:16:47,620 --> 00:16:54,860
+البني آدم لا يسوي شيء كام تحتاجي لأن تبعدي عن
+
+175
+00:16:54,860 --> 00:17:00,620
+الكهربا؟ نص ملي تحركي إيدك أو جسمك نص ملي، مظبوط؟
+
+176
+00:17:00,620 --> 00:17:05,360
+عشان تبعدي عن إيش؟ سلك الكهربا، تقدري؟ تقدري تحركي
+
+177
+00:17:05,360 --> 00:17:09,910
+حالك نص ملي؟شايف هالضخام و العامل حال الواحد فيش
+
+178
+00:17:09,910 --> 00:17:14,070
+في الدنيا اللى هو لا يستطيع حين قدر الله سبحانه
+
+179
+00:17:14,070 --> 00:17:17,970
+وتعالى ان يحرك حاله نص مدى فالكهربا يا أخوات
+
+180
+00:17:17,970 --> 00:17:23,390
+المجدات إذا مسكت الإنسان بتوقف الجهاز العصب فبيجعف
+
+181
+00:17:23,390 --> 00:17:27,130
+فبيقولوا رمته الكهربا هي مارمتهوش الكهربا هو فقد
+
+182
+00:17:27,130 --> 00:17:32,860
+اتزانه بيقدر يظل متزن واقف الواحدفبيجى .. و لما
+
+183
+00:17:32,860 --> 00:17:36,440
+بيجى بتقول نفضته الكهربا او رمته الكهربا طبعا هو و
+
+184
+00:17:36,440 --> 00:17:41,900
+مصيبه يا بترميه و بيبعد عن السلك و بفك يا بيكون
+
+185
+00:17:41,900 --> 00:17:45,640
+جسمه معرق او مبلل و بيجي فوق السلك و بتخلص عليه
+
+186
+00:17:45,640 --> 00:17:51,060
+مظبوط؟ و كتير يعني في حالات صابتهم الكهربا و ماتوا
+
+187
+00:17:51,060 --> 00:17:57,820
+بفحن تفحين فالصدمة الكهربائية ايش بتساوي بتعمل
+
+188
+00:17:57,820 --> 00:18:03,500
+تأيين في الدمايش يعني تأيون في الدم؟ ايش يعني
+
+189
+00:18:03,500 --> 00:18:09,360
+تأيون؟ الموجب بيروح على شجة والسالب بيروح على شجة
+
+190
+00:18:09,360 --> 00:18:15,520
+مين عارف ايش الملح؟ ايش الملح؟ ال sodium ال
+
+191
+00:18:15,520 --> 00:18:22,420
+chloride مش هيك؟ مش هيك الملح؟ ال sodium موجب و ال
+
+192
+00:18:22,420 --> 00:18:28,500
+chloride سالب لو جيبنا ال sodium لحاله و ناكله
+
+193
+00:18:28,500 --> 00:18:32,850
+بعملنا مشكلة؟و نجيبنا الكلور لحاله و ناكله بيعملنا
+
+194
+00:18:32,850 --> 00:18:37,810
+مشكلة لكن التاني مع بعض مهمات
+
+195
+00:18:37,810 --> 00:18:41,970
+.. مهمات للبني آدم ما بتعرفيش تاكل ولا أكلة حتى
+
+196
+00:18:41,970 --> 00:18:46,390
+الحلويات لما بنعملها بالرش رشة ملعق رشة بسيطة،
+
+197
+00:18:46,390 --> 00:18:51,190
+نصبوت؟ بتديها طعم أسبق فسبحان الله يعني لما يصير
+
+198
+00:18:51,190 --> 00:18:58,130
+الدم الموجب على شجة و السالب على شجة إيش بيصير؟ مش
+
+199
+00:18:58,130 --> 00:19:02,700
+دمفلو أجل المخ السالي بيبقى شجة و الموجة بيبقى شجة
+
+200
+00:19:02,700 --> 00:19:08,160
+بيستفيد منه المخ بيستفيدش منه المخ فإذا الصدمة
+
+201
+00:19:08,160 --> 00:19:13,540
+الكهرباية ضايد ايش وراكم حكينا عن الصدمة التسممية
+
+202
+00:19:13,540 --> 00:19:18,620
+والصدمة الكهرباية الصدمة الكيميائية الصدمة
+
+203
+00:19:18,620 --> 00:19:22,800
+الكيميائية الصدمة الكيميائية بتختلف عن الصدمة
+
+204
+00:19:22,800 --> 00:19:28,930
+التسممية في ايش ان الإنسان باخد أشياءمواد كيميائية
+
+205
+00:19:28,930 --> 00:19:33,730
+جسمه مابيقبلهاش يعني واحد عنده حساسية بنسالين
+
+206
+00:19:33,730 --> 00:19:41,310
+بنسالين مادة كيميائية بتفيد كل الناس إلا جزء بسيط
+
+207
+00:19:41,310 --> 00:19:46,830
+من الناس لو أخد إبرة بنسالين وجسمه مابيقبلش هذه
+
+208
+00:19:46,830 --> 00:19:51,630
+الإبرة بيخش فيه مشكلة أنا أذكر يوم من الأيام في إن
+
+209
+00:19:51,630 --> 00:19:56,740
+بنت جارةمنت صغيرة هيك يعني جرتنا الصبح بشوفها و
+
+210
+00:19:56,740 --> 00:20:00,600
+الضغط بشوفها و بتخرجت معاها زغنونة جات بنات بناتي
+
+211
+00:20:00,600 --> 00:20:06,020
+يعني او جناتي ولادي فمار جين هي و أمها من الشارع
+
+212
+00:20:06,020 --> 00:20:09,780
+بتطلع في البنت مش هذه البنت اللي بشوفها كل يوم
+
+213
+00:20:09,780 --> 00:20:14,540
+واقف يا حج مهلا وين باقي؟ قالت والله جايين من
+
+214
+00:20:14,540 --> 00:20:18,180
+عيادة الوكالة ايش سويت البنت؟ قال ده جناها قبرة
+
+215
+00:20:18,180 --> 00:20:23,710
+بنسالين بقوا أيام هيدقوا بنسالين قبرقلت لها بس بس
+
+216
+00:20:23,710 --> 00:20:28,410
+البنت بدها تموت و أخدت البنت و قال الله كريم أنه
+
+217
+00:20:28,410 --> 00:20:33,270
+عندي أدوية أعطيتها مضاد للحساسية و اتحسن وضعها و
+
+218
+00:20:33,270 --> 00:20:37,270
+الحين صرت لما كبرت يقولوا ليها دي بنتك فكان
+
+219
+00:20:37,270 --> 00:20:42,470
+بالإمكان أنها تخش في صدمة كيميائية أنا أذكر من مدة
+
+220
+00:20:42,470 --> 00:20:50,530
+في الوكالة في خانوس أوفح ما أذكر بالظبط وينبدل ما
+
+221
+00:20:50,530 --> 00:20:56,930
+يطعموا الأطفال بتطعيم صحيح طعموهم بأنسولين زجاجة
+
+222
+00:20:56,930 --> 00:21:02,610
+الأنسولين زيها بالظبط زي زجاجة التطعيم طبعا
+
+223
+00:21:02,610 --> 00:21:09,430
+الأنسولين بضيع السكر في الجسم المخ بتغذع السكر
+
+224
+00:21:09,430 --> 00:21:16,910
+فبطل يصل لمخ الأطفال سكر بعضهم مات وبعضهم أصيب
+
+225
+00:21:16,910 --> 00:21:24,640
+بإعاقات وبعضهم لحكوهه؟ اه اه بعضهم أصيب بإعاقات و
+
+226
+00:21:24,640 --> 00:21:31,560
+وعدهم مات و بعضهم ايش لحقوه نتيجة انه بطل يصل بصل
+
+227
+00:21:31,560 --> 00:21:36,300
+دم بس مش فيه سكر بصل دم بس مش فيه سكر كله هذا اسم
+
+228
+00:21:36,300 --> 00:21:40,600
+الصدمة ايش كيميائية فالصدمة الكيميائية يعني بياخد
+
+229
+00:21:40,600 --> 00:21:48,020
+واحد حاجات بتخرب الدم او بتخلي الجسم مايستفيدش منه
+
+230
+00:21:48,020 --> 00:21:49,500
+طبعا
+
+231
+00:21:51,390 --> 00:21:56,810
+الصدمة بتخلي الإنسان في حاجة زيادة لل Oxygen لأنه
+
+232
+00:21:56,810 --> 00:21:59,830
+بيصير ضربات القلب زيادة و بعدين لازم إذا في نزيف
+
+233
+00:21:59,830 --> 00:22:05,610
+حد حاد بدك توقفيه عشان تحافظي على حجم الدم كمان
+
+234
+00:22:05,610 --> 00:22:09,050
+إذا كان هناك إصابات في الرأس أو الرقبة لازم المصاب
+
+235
+00:22:09,050 --> 00:22:13,850
+يظلوا مايتحركش لأنه ممكن أي حركة في حالة إصابة
+
+236
+00:22:13,850 --> 00:22:19,810
+الرقبة تعمل مشاكل الصدمة هذه كيف بدنا نتعامل معاها
+
+237
+00:22:25,170 --> 00:22:32,590
+هدفي اوصل دم للاجهزة الحيوية للمخ كيف بدأ اوصل دم
+
+238
+00:22:32,590 --> 00:22:38,230
+للمخ الحاجة الأولى بده حسن دورة دمائي بالقاتل في
+
+239
+00:22:38,230 --> 00:22:44,570
+نزيف بده وقفه في نزيف بده وقفه خدنا كيف نوقف نزيف
+
+240
+00:22:44,570 --> 00:22:49,950
+ذاكرات مش صعب ولا صعب ايش الأدوات اللي استخدمناها
+
+241
+00:22:51,940 --> 00:22:57,200
+المتوفر حولي نظموت عشان اوقف النظيف استخدمت أدوات
+
+242
+00:22:57,200 --> 00:23:02,840
+المتوفرة حولي فعشان
+
+243
+00:23:02,840 --> 00:23:09,080
+أحسن الدورة الدموية بدأ أسيطر عن النظيف اذا
+
+244
+00:23:09,080 --> 00:23:14,960
+كان سيطرت على النظيف وفي نقص سوائل والمصاب يستطيع
+
+245
+00:23:14,960 --> 00:23:20,900
+الشرب بدأ اعوضله السوائل ده سجينبدأ أزجيه سوائل
+
+246
+00:23:20,900 --> 00:23:26,720
+طبعا السوائل هذه بتعودش الدم انما ايش بتعمل بتزيد
+
+247
+00:23:26,720 --> 00:23:32,940
+حجم .. بتزيد حجم الدم الحين في الحروب او في
+
+248
+00:23:32,940 --> 00:23:38,000
+الإصابات المستشفى بيطلعوا يقولوا ايش بدناه يا
+
+249
+00:23:38,000 --> 00:23:42,200
+جماعة اللي عنده استعداد يتبرع بالدم يتفضل على
+
+250
+00:23:42,200 --> 00:23:48,330
+مستشفى كده طب يعني مافيش عندهم حليل؟عندهم محليل
+
+251
+00:23:48,330 --> 00:23:52,790
+لكن المحليل المحليل بتعوضش الدم الحاجة الأمثل
+
+252
+00:23:52,790 --> 00:23:55,850
+لتعويض الدم ايش او الحاجة الوحيد لتعويض الدم اللي
+
+253
+00:23:55,850 --> 00:24:02,750
+هو ايش الدم لا شيء يعوض الدم مثل الدم وإلا كان
+
+254
+00:24:02,750 --> 00:24:07,170
+حطينا وبناشي نغلب حالنا فاحنا بدنا نسيطر على
+
+255
+00:24:07,170 --> 00:24:13,490
+النزيف ونعطل بني آدم سوائل إذا بيشرب وصاحي عشان
+
+256
+00:24:13,490 --> 00:24:20,210
+ايشانزيد حجم الدم حتى يصل الى اقرب مركز طبي طبعا
+
+257
+00:24:20,210 --> 00:24:23,710
+اذا في اصابات في الرقب او في العنق او حاجة زي هيك
+
+258
+00:24:23,710 --> 00:24:28,110
+ممنوع يتحرك لانه اذا اتحركت الرجب وانقطع النقاع
+
+259
+00:24:28,110 --> 00:24:34,230
+الشوكي كل ما تحته بنشل والتنفس وضربات الجلب في
+
+260
+00:24:34,230 --> 00:24:37,910
+الرجل التنفس وضربات الجلب في الرجل كمان انا بديش
+
+261
+00:24:37,910 --> 00:24:42,530
+حتى لو في الظهر بديش اشيل البنا آدم يعنى ولا لأطلع
+
+262
+00:24:42,530 --> 00:24:46,870
+بالزلمة و طلع مشلول، إيش عملنا؟ لأ بزيادة خمس
+
+263
+00:24:46,870 --> 00:24:52,290
+دقايق، بزيادة روبع ساعة حتى يأتي الناس المتخصصين و
+
+264
+00:24:52,290 --> 00:24:57,450
+تيجي سيارة الأسعاف و بدي أهتم في المصاب ككل، احنا
+
+265
+00:24:57,450 --> 00:25:03,390
+جولنا البني آدم كللا يتجزأ مظبوط نحافظ على كل
+
+266
+00:25:03,390 --> 00:25:10,430
+أجزاء الإنسان بحيث انه ما نهتم في جزء لون الآخر
+
+267
+00:25:10,430 --> 00:25:16,830
+لأنه إذا اشتكى منه عضو سائر الجسد بالسهري و الحمض
+
+268
+00:25:16,830 --> 00:25:24,150
+كيف بدنا نسعى في الصدمة قولنا واحد عندي عنده صدمة
+
+269
+00:25:24,150 --> 00:25:26,910
+كيف بدنا نسعى في الصدمة
+
+270
+00:25:30,730 --> 00:25:39,850
+أول حاجة أي حركة للي عنده صدمة تعتبر جهد الجهد هذا
+
+271
+00:25:39,850 --> 00:25:46,530
+ماذا يحتاج طاقة طاقة بدها دم مافيش عنده طاقة زيادة
+
+272
+00:25:46,530 --> 00:25:52,570
+ولا في عنده إمكانية انه يعطي الدم كفاية فبحاول ان
+
+273
+00:25:52,570 --> 00:25:58,190
+اخليه هادي بحاول اخليه هادي انا بالزمن الدم وين؟
+
+274
+00:25:58,190 --> 00:26:08,230
+في المخوفى القلب كل بساطة بلزمنيش فى الرجلين او
+
+275
+00:26:08,230 --> 00:26:13,410
+حتى الادين دم فبروحش على مدى بنايم المصاد وبرفع
+
+276
+00:26:13,410 --> 00:26:21,450
+رجليه هذا تلاتين سانتي فوق مستوى القلب ايش سويت
+
+277
+00:26:21,450 --> 00:26:26,930
+انا ساعدت ان الدم اللى فى الرجلين هذا يروح للدورة
+
+278
+00:26:26,930 --> 00:26:31,560
+الدموية الأساسية ايش الأدوات اللى استخدمتهافيش ولا
+
+279
+00:26:31,560 --> 00:26:37,460
+حاجة عندي بحطه اجريه برفعهم بحطه جنب حيط ماخدين
+
+280
+00:26:37,460 --> 00:26:45,320
+بالكواكير؟ بحط اجريه جنب حيط ب .. حجر block كرسي
+
+281
+00:26:45,320 --> 00:26:55,600
+حجر عادي اي شي حواليا ممكن جدا ارفع رجليه على
+
+282
+00:26:55,600 --> 00:27:05,300
+المكان هذا و اخل الدم يرجعباهتم في الملاحظات اللى
+
+283
+00:27:05,300 --> 00:27:11,000
+هى علامات الحياة بسميها اللى هى النبض وش قلنا و
+
+284
+00:27:11,000 --> 00:27:20,100
+التنفس و باهتم بإنه يعني صاحي باهتم إنه ما نتجش و
+
+285
+00:27:20,100 --> 00:27:23,820
+خنج نفسه تانى كيه بده اهتم إنه ما نتجش و خنج نفسه
+
+286
+00:27:23,820 --> 00:27:30,740
+تانى؟ بده أحطه على جهة وجهه بحط وجهه على جهةبالظبط
+
+287
+00:27:30,740 --> 00:27:36,640
+الطفل الصغير بعد ما طرده أمه الأصل إيش؟ طره .. أول
+
+288
+00:27:36,640 --> 00:27:41,000
+حاجة إيش؟ اضطره اللي بيقولوا عن ال .. ال .. ال ..
+
+289
+00:27:41,000 --> 00:27:41,300
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+290
+00:27:41,300 --> 00:27:41,320
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+291
+00:27:41,320 --> 00:27:41,720
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+292
+00:27:41,720 --> 00:27:42,500
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+293
+00:27:42,500 --> 00:27:42,700
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+294
+00:27:42,700 --> 00:27:43,520
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+295
+00:27:43,520 --> 00:27:43,560
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+296
+00:27:43,560 --> 00:27:46,780
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+297
+00:27:46,780 --> 00:27:46,780
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+298
+00:27:46,780 --> 00:27:53,370
+.. ال .. ال .. ال .. ال .. ال .. ال .. الفممكن جدا
+
+299
+00:27:53,370 --> 00:27:57,110
+صدقوا صدقوا أجانا أكتر من مرة أطفال في سن تلاتة
+
+300
+00:27:57,110 --> 00:28:01,870
+إلى شهرين إلى أربعة ميتين عن المستشفى ناتج بعد
+
+301
+00:28:01,870 --> 00:28:05,700
+الضاعةو صحيت أمه في الليل الولد بعد ساعتين تلاتة
+
+302
+00:28:05,700 --> 00:28:09,320
+بصحى ماصحيش بعد أربع خمس ساعات طلع في الولد اللي
+
+303
+00:28:09,320 --> 00:28:13,020
+الولد أزرق أزرق وشريطة و جابوه يجوا في المستشفى من
+
+304
+00:28:13,020 --> 00:28:18,840
+إيش؟ ردعته سيادته ونامت ماطرعتوش محططوش على
+
+305
+00:28:18,840 --> 00:28:22,300
+الجامعه طبعا الولد صغير مش سهل يحرك حد بيمين ولا
+
+306
+00:28:22,300 --> 00:28:28,940
+شمال أو بالأعلى مش همش بسكروا خنقوا خنقوا سكروا
+
+307
+00:28:28,940 --> 00:28:39,840
+خنقوا بعدين؟نعم، إذا نهتم بإنه نجيسله الملاحظات،
+
+308
+00:28:39,840 --> 00:28:45,380
+نشوف النبض نتابعهتوقف القلب بتعمله تدليق قلب، توقف
+
+309
+00:28:45,380 --> 00:28:51,920
+النفس بتعمله تنفس صناعي بنرفع الأرجل إلى أعلى عشان
+
+310
+00:28:51,920 --> 00:28:56,940
+الدم اللي في رجليه يرجع إلى الدورة الدموية إلا إذا
+
+311
+00:28:56,940 --> 00:29:01,080
+في طبعا أي مشكلة يعني مثلا واحد أجره مش سهل أرفعها
+
+312
+00:29:01,080 --> 00:29:02,340
+بحاول أني مارفعهاش
+
+313
+00:29:09,820 --> 00:29:13,600
+مين .. أنت عندك ولاد؟ مين متجوز و عنده ولاد يبقى
+
+314
+00:29:13,600 --> 00:29:20,320
+عندك؟ إيش بتنام أبنك؟ سهل ولا صاهر؟ من الأحلى
+
+315
+00:29:20,320 --> 00:29:23,100
+مايمكن أنه ينام الطفل علي جنبي أختي الله يرضى عليك
+
+316
+00:29:23,100 --> 00:29:27,760
+حطي ايه هيك على كتفه و نايميه خايف يرجع تانى حط
+
+317
+00:29:27,760 --> 00:29:32,040
+وسادة ورا بقى وسادة صغيرة هذه كمان واحدة واحدة
+
+318
+00:29:32,040 --> 00:29:34,600
+لراسه وواحدة جنبه بلاش حتى اللي عنده راسه حطيها
+
+319
+00:29:34,600 --> 00:29:41,600
+ورا ده بتلاقيه نام ورتكز وليش رايك؟احنا بدنا بعد
+
+320
+00:29:41,600 --> 00:29:46,100
+الرضاعة مباشرة ندشنه زى ما قالت الأخت او بلاش
+
+321
+00:29:46,100 --> 00:29:51,740
+ندشنه احنا بقول نطرعه ماشي بعد ما نطرعه نايمه على
+
+322
+00:29:51,740 --> 00:29:58,160
+جنبه لإن الأكل بقعد فترة في المعدة ماشي بعد الفترة
+
+323
+00:29:58,160 --> 00:30:02,240
+هذه وين بروح؟بتفضل المعدة وبروح لأمعاق طبعا بنادر
+
+324
+00:30:02,240 --> 00:30:05,920
+أملي كبير لما ياكل واجبة دسمة تقعد حوالي أربع
+
+325
+00:30:05,920 --> 00:30:10,760
+ساعات في المعدة تعالى أنت تغدى مفتول و لحمة ماجيش
+
+326
+00:30:10,760 --> 00:30:15,220
+ع بالك تتعشى بقعدنا المغرب و أنت شابعانة مظبوط طب
+
+327
+00:30:15,220 --> 00:30:21,020
+كل رز كمان ساعة إلا ربع بيصير بدك تاكل تاني ماشي
+
+328
+00:30:21,020 --> 00:30:24,320
+فالحليب ممكن يقعدله حوالي ساعتين ساعتين و نص في
+
+329
+00:30:24,320 --> 00:30:29,030
+المعدة بعد هيك حطي على أي جنب بدكففي فترة هادى
+
+330
+00:30:29,030 --> 00:30:33,810
+ممكن ينتج مش الأطفال بينتجوا وما بيروحوا النتقل
+
+331
+00:30:33,810 --> 00:30:41,510
+بده سكر مجرد التنفس .. بسكر مجرد التنفس ينخنق واخد
+
+332
+00:30:41,510 --> 00:30:45,770
+بالك فبنحط الطفل على جنبه طبيعى يعنى مابتقدرش
+
+333
+00:30:45,770 --> 00:30:50,810
+تتخلى لحاله بتحط وسادة صغيرة ورا دهره بتحطيها بدون
+
+334
+00:30:50,810 --> 00:30:54,530
+وسادة ولا لأ؟ بدون وسادة بس بيبقى كده على جانبك
+
+335
+00:30:54,530 --> 00:31:02,700
+نعمإذا بنسيطر عن نزيف بنوقف النزيف طريقتنا و
+
+336
+00:31:02,700 --> 00:31:08,400
+بنتابع الملاحظات اللي هي العلامات الحيوية اللي هي
+
+337
+00:31:08,400 --> 00:31:15,560
+النبض و التنفس و بنرفع إجريه إلى أعلى حتى يعود
+
+338
+00:31:15,560 --> 00:31:19,700
+الدم اللي في الأرجل إلا إذا كان فيه مانع اللي هيك
+
+339
+00:31:19,700 --> 00:31:20,100
+شغله
+
+340
+00:31:23,110 --> 00:31:27,950
+مهم جدا انه نحافظ على درجة حرارة الإنسان ليش
+
+341
+00:31:27,950 --> 00:31:33,370
+بنحافظ على درجة حرارة الإنسان؟ في الصدمة مهم جدا
+
+342
+00:31:33,370 --> 00:31:37,910
+أنه نحافظ على درجة حرارة الإنسان لأنه في الحرب إذا
+
+343
+00:31:37,910 --> 00:31:38,470
+دفعنا بزيادة
+
+344
+00:31:49,630 --> 00:31:55,850
+هيصير الأوعية الدموية الطرفية ملانة بالدم على حساب
+
+345
+00:31:55,850 --> 00:32:00,850
+الأجهزة الحيوية على حساب الأجهزة الحيوية طب وان
+
+346
+00:32:00,850 --> 00:32:11,870
+كان سجعة في البرد نعم ايش بيصير احنا
+
+347
+00:32:11,870 --> 00:32:14,750
+مش كتير لازم ندم في الأطراف بس لا بدناش إياه يسجع
+
+348
+00:32:14,750 --> 00:32:16,450
+ليش بدناش إياه يسجع
+
+349
+00:32:23,700 --> 00:32:27,600
+فى البرد الشديد يا أخوات المجدات بيصير الواحد
+
+350
+00:32:27,600 --> 00:32:36,200
+يرجع، عمار كده شوفت ولا ده صغير بتق، إيش يعني بتق؟
+
+351
+00:32:36,200 --> 00:32:37,240
+سجعان كتير
+
+352
+00:32:40,470 --> 00:32:46,330
+بالظبط لما يبقى حرارته مرتفعة برضه بيصير إيه؟ يرجف
+
+353
+00:32:46,330 --> 00:32:52,010
+فاحنا بناشيها لا يسخن ولا يبرد ان سخن بيكون
+
+354
+00:32:52,010 --> 00:32:56,030
+الأعضاء الجانبية والطرفية اتوسع الأوعية الدموية
+
+355
+00:32:56,030 --> 00:33:01,890
+فيها وانتلت دم على حساب الأجهزة الحيوية إذا برد
+
+356
+00:33:01,890 --> 00:33:07,950
+بده يدف جسمه بيصير يرجف عشان يرجف إيش بده؟بدو دم
+
+357
+00:33:07,950 --> 00:33:12,410
+فى الأطراف لما تبدى تصير فى حركة شديدة برضه أخد
+
+358
+00:33:12,410 --> 00:33:17,050
+الدم وين من الجهاز الحيوي اللى هو المخ و القلب
+
+359
+00:33:17,050 --> 00:33:22,150
+ووده وين الأطراف عشان هيك بدي أحافظ على حرارة
+
+360
+00:33:22,150 --> 00:33:29,390
+الجسم بحيث بمعنى كيف بدي أحافظ على حرارة الجسم إذا
+
+361
+00:33:29,390 --> 00:33:35,070
+كانت الدنيا برد بدي أغطيه بغطى محترم وإذا كانت
+
+362
+00:33:35,070 --> 00:33:39,480
+الدنيا شوبولابس أوائة كتير بتخفف عنه أقولكوا
+
+363
+00:33:39,480 --> 00:33:45,540
+هالقصة الصغيرة أنا كنت في يوم من الأيام بأشتغل في
+
+364
+00:33:45,540 --> 00:33:48,580
+أصدقاء المريض عارفين أصدقاء المريض؟ هي اليوم
+
+365
+00:33:48,580 --> 00:33:57,740
+بتجامعة وطبعا بعد الظهر أجانا واحد متخلف بيشتغل في
+
+366
+00:33:57,740 --> 00:34:01,780
+البلدية زمان كان يعني البلدية بيشتغلوا فيها اليوم
+
+367
+00:34:01,780 --> 00:34:05,910
+اللي بيشتغلوا في البلدية معاهم دكتورو معاهم
+
+368
+00:34:05,910 --> 00:34:10,490
+مهندسين و بترجى رجى مسكين عشان ايش يشتغل يكنس
+
+369
+00:34:10,490 --> 00:34:15,090
+الشوارع مظبوط بس يلاقي زمان بقوا يترجوا الناس رجع
+
+370
+00:34:15,090 --> 00:34:20,430
+انهم يشتغلوا و ماحدش يرضى فأجانا الراجل هذا متخلف
+
+371
+00:34:20,430 --> 00:34:25,210
+و مشغلين في البلدية طبعا ينظف الشوارع الدنيا شهر
+
+372
+00:34:25,210 --> 00:34:34,350
+سبعة تقريبا و شوب فهذا في الشوب صار عنده صدمةنتيجة
+
+373
+00:34:34,350 --> 00:34:39,570
+العراج ونتيجة كذا وفقدان السواقل فلما جابوه عندنا
+
+374
+00:34:39,570 --> 00:34:46,970
+صدقوا صدقوا اكتر من خمستاشر بنطلون شلحنا لابس اكتر
+
+375
+00:34:46,970 --> 00:34:51,530
+من خمستاشر بنطلون كيف يعني هو بيشتغل في البلدية
+
+376
+00:34:51,530 --> 00:34:56,810
+بيكنس وكل ما هو بيكنس ع الزبالة بيلاقي بنطلون ايش
+
+377
+00:34:56,810 --> 00:35:01,910
+بيساوي فيه بيلبسوه كيف البنطلون اللي برتمع الزبالة
+
+378
+00:35:03,650 --> 00:35:10,550
+زي الشادر مش بس وسخه بس زي ال nylon من كتر الوساخة
+
+379
+00:35:10,550 --> 00:35:14,770
+فلبس فوق الخمستاشر او ستاشر بنطلون ايش عمل ان
+
+380
+00:35:14,770 --> 00:35:20,310
+هادول خل ان جسمه يصير حامي جدا وصار فيه توسف
+
+381
+00:35:20,310 --> 00:35:25,010
+الأوعي الترفية خش في صدمةماخدين بالكوا كيف
+
+382
+00:35:25,010 --> 00:35:29,830
+فبقولكوا جينا نعالجوا يعني شلّح البنطلون الأول
+
+383
+00:35:29,830 --> 00:35:35,370
+التاني التاني حوالي 15 بنطلون رفعنا عنه في هذاك
+
+384
+00:35:35,370 --> 00:35:40,710
+الوجد فدخلنا انه فعلا دخلنا انه في صدمة يعنى بمعنى
+
+385
+00:35:40,710 --> 00:35:45,750
+انه بدنا نحافظ على درجة حرارة الجسم إذا بده شوية
+
+386
+00:35:45,750 --> 00:35:52,440
+تدفئة بسيطة عشان مايرجفش بدنا ندفيه وإذا كانالجو
+
+387
+00:35:52,440 --> 00:35:55,960
+بارد بدنا ندفيه وإذا كان الجو سخن سخن بدنا نخفف
+
+388
+00:35:55,960 --> 00:36:02,720
+عنه الملابس وإلا بيكون في مشكلة تخلى الأوعية
+
+389
+00:36:02,720 --> 00:36:08,260
+الدموية الطرفية تنتلي على حساب اللي هو القلب وذا
+
+390
+00:36:08,260 --> 00:36:13,100
+لازم نأمر طبعا العمل تنفس صناعي بدنا نعمله تنفس
+
+391
+00:36:13,100 --> 00:36:18,980
+صناعي احنا قولنا صدمة الكهربا كيف بتصير؟ مين
+
+392
+00:36:18,980 --> 00:36:19,900
+عارفها؟
+
+393
+00:36:22,340 --> 00:36:29,700
+مين تقولي كيف تصير؟ بتصير إنه بيفقد الجسم إحساسه
+
+394
+00:36:29,700 --> 00:36:34,740
+الجهاز العصبي بيفقد إحساسه مافيش عند الإنسان
+
+395
+00:36:34,740 --> 00:36:40,800
+إمكانية إنه يتحرك milli واحد الحاجة التانية بيصير
+
+396
+00:36:40,800 --> 00:36:49,440
+في مكان يعني أنا أذكر في حياتي شوفت مرتين ناس من
+
+397
+00:36:49,440 --> 00:36:50,840
+وين طبعا الكهربا بتجيناها
+
+398
+00:36:53,650 --> 00:36:57,750
+تجينا من الشركة يعني من الكهربا تلي البيت كمان
+
+399
+00:36:57,750 --> 00:37:04,250
+ممكن تجينا كهربا من البرج من البرج نفسه عارفين أنا
+
+400
+00:37:04,250 --> 00:37:09,770
+شوفت مرتين شوفت مرتين في حياتي فقط شوفت مرتين في
+
+401
+00:37:09,770 --> 00:37:18,720
+حياتي فقط ناس ماتوا من البرجناس من رفح وناس من
+
+402
+00:37:18,720 --> 00:37:23,400
+خالي ونص طبعا في يوم بتبقى الدنيا مطر شديد وفي
+
+403
+00:37:23,400 --> 00:37:29,880
+راعد وبرج شديد وطبعا وماشيين اتنين منهم كانوا رعاد
+
+404
+00:37:29,880 --> 00:37:34,380
+غنم في الخلاه واتنين تانين كانوا ماشيين على شط
+
+405
+00:37:34,380 --> 00:37:45,050
+البحر ماشي فتعرضوا الى سعقة كهربائية نتيجةاللي هو
+
+406
+00:37:45,050 --> 00:37:50,990
+البرج البرج عبارة عن إيش؟
+
+407
+00:37:50,990 --> 00:37:57,050
+التقاء غينتين واحد موجبة وواحد سالبة فبولده كهربا
+
+408
+00:37:57,050 --> 00:38:02,630
+جوية جدا بتعمل الشرارة وفي نفس اللحظة بتعمل الصوت
+
+409
+00:38:02,630 --> 00:38:08,390
+بس الشرارة بتصلنا قبل الصوت لإنها أسرع من الصوت
+
+410
+00:38:08,390 --> 00:38:13,110
+دايما بعد البرج بشويه بتسمع إيشالراعد فالراعد هو
+
+411
+00:38:13,110 --> 00:38:19,010
+صوت التقاء اللى هى السحابتين محملة بسالب وموجب و
+
+412
+00:38:19,010 --> 00:38:24,170
+بتعمل كهربا و بتعمل ما يسمى بالراعد فالكهربا اللى
+
+413
+00:38:24,170 --> 00:38:29,370
+تبعت المنزل اللى من الشركة ممكن تعمل تفقد الإنسان
+
+414
+00:38:29,370 --> 00:38:34,890
+جهازه العصبي بطل يحس لدرجة زى ما قلنا قداش بدك
+
+415
+00:38:34,890 --> 00:38:39,470
+تطيبي من المأذق أو تطلع من هذا المأذق بدك ملي
+
+416
+00:38:39,470 --> 00:38:45,460
+ترفعي إيدك بتقدريعارفين قصة ال .. الراجل اللى صار
+
+417
+00:38:45,460 --> 00:38:50,040
+عنده مشكلة ف .. و هو بحلم فبسأله ايش ممكن يعمل؟
+
+418
+00:38:50,040 --> 00:38:54,260
+يصحى من النوم، بروح المعزقة، مش هيك؟ فانت فى معزقة
+
+419
+00:38:54,260 --> 00:38:59,620
+الكهربا، ايش بدك؟ بدك ملي يتحركى تبعد عن الكهربا،
+
+420
+00:38:59,620 --> 00:39:04,300
+مظبوط؟ لا يستطيع انه يتحرك الملي الحاجة التانية
+
+421
+00:39:04,300 --> 00:39:06,900
+اللى بتسير انه ايش بيثير؟
+
+422
+00:39:10,550 --> 00:39:16,450
+بصير تعيون في الدم زى ما حكينا الصوديوم بروحها شجة
+
+423
+00:39:16,450 --> 00:39:20,370
+والكلور بروحها شجة الحاجات الموجبة في الدم بروحها
+
+424
+00:39:20,370 --> 00:39:25,750
+شجة والحاجات السلب بروحها شجة صار الدم ايش مش دم
+
+425
+00:39:25,750 --> 00:39:34,010
+بشتغلش شغله كدم متجمع تمام تمام الصدمة
+
+426
+00:39:35,270 --> 00:39:39,270
+أما عصبية وتسبب الوفاة لشدتها وقد تكون صدمة قلبية
+
+427
+00:39:39,270 --> 00:39:45,230
+نتيجة تأثير اللي هو الكهربا على عضلة القلب احنا
+
+428
+00:39:45,230 --> 00:39:49,270
+كثير من الناس بيصير عنده توقف قلب بنعمله صدمة
+
+429
+00:39:49,270 --> 00:39:53,710
+كهرباية، مظبوط؟ بس قداشة صدمة الكهرباية مرة واحدة
+
+430
+00:39:53,710 --> 00:40:00,970
+لثانية وموزونة فممكن صدمة الكهربا الزيادة تأثر على
+
+431
+00:40:00,970 --> 00:40:06,180
+عضلة القلب، ماشي؟اسعاف اصابات اللي هو التيار
+
+432
+00:40:06,180 --> 00:40:10,860
+الكهربى كيف احنا بنساوي لو واحد عنده صدمة بالتيار
+
+433
+00:40:10,860 --> 00:40:13,940
+الكهربى؟
+
+434
+00:40:13,940 --> 00:40:17,940
+بدنا نبعد عنه الكهربى او نبعده .. او نبعده عن
+
+435
+00:40:17,940 --> 00:40:23,680
+الكهربى طبعا يا أخوتي الماجدات اذا انت جيت مسكت
+
+436
+00:40:23,680 --> 00:40:28,380
+طبق المصاب بالكهربى بيصير هو عبارة عن سلك بيوصل
+
+437
+00:40:29,460 --> 00:40:35,340
+عبارة عن خط كهربي بوصل فانت لازم يا اما بتبعد
+
+438
+00:40:35,340 --> 00:40:41,740
+الكهربا عنه بانك تطفل الأمان او تطفل المفتاح او
+
+439
+00:40:41,740 --> 00:40:45,860
+بدك تبعدي عن الكهربا كيف بده ابعد عن الكهربا؟ بده
+
+440
+00:40:45,860 --> 00:40:50,620
+اجيب أداة غير موصلة وماتكونيش سياتك حافية لأن
+
+441
+00:40:50,620 --> 00:40:54,600
+سياتك اذا كنت حافية ممكن جدا الكهربا بتكون واصل
+
+442
+00:40:54,600 --> 00:41:00,160
+الأرض بتلاحظ ان انت تيجي بتفتح تلاجة مثلاتلاجة
+
+443
+00:41:00,160 --> 00:41:05,820
+بتحس انه فيه مس تكتش في نفسك انك لابسة شبشب مبلل
+
+444
+00:41:05,820 --> 00:41:11,440
+او مش لابسة شبشب خالص في المطبخ مش هيك بيجي أخوك
+
+445
+00:41:11,440 --> 00:41:14,520
+بيجي أمك بيجي حدا بمسك و بقولك يا عم مش مبين الشيء
+
+446
+00:41:14,520 --> 00:41:21,220
+ليش؟ لأنه معزول عن الأرض الأصل مايكونش للمعزول ولا
+
+447
+00:41:21,220 --> 00:41:26,100
+لغير المعزول لكن إذا كان في عزل عن الأرض باكتملش
+
+448
+00:41:26,100 --> 00:41:30,360
+الدائرةعشان تكتمل الدائرة لازم يكون في توصيل بين
+
+449
+00:41:30,360 --> 00:41:36,080
+الأرض والجسم أو في مسك للخطين في مسك للموجب
+
+450
+00:41:36,080 --> 00:41:41,460
+والسالب .. مين عندك المهندس الكهربا؟ مش ولا حد ..
+
+451
+00:41:41,460 --> 00:41:46,640
+السالب والموجب إذا اتصلن في جسم الإنسان بنزلش
+
+452
+00:41:46,640 --> 00:41:51,740
+الأمان أمان الأفراد بنزلش بنزل إذا اتصل الجسم
+
+453
+00:41:51,740 --> 00:41:56,860
+بالأرضفإذا اتصل الجسم بالأرض بنزل الأمان فالأمان
+
+454
+00:41:56,860 --> 00:42:01,060
+بيصير مالوش لزوم لما أنت بتمسك قطين وبنقل عليه
+
+455
+00:42:01,060 --> 00:42:06,340
+تعيين الكهربا الحاجة الأولى بنعملها أن نفصل التيار
+
+456
+00:42:06,340 --> 00:42:10,960
+الكهربا أو نزيحه عن الكهرباكيف نقول؟ زي ما قالت
+
+457
+00:42:10,960 --> 00:42:17,020
+أختنا، بنجيب حاجة عازلة، بنرفع فيها .. بنزيح فيها
+
+458
+00:42:17,020 --> 00:42:25,260
+السلك الكهربا عن الإنسان و بيكون هيك المصدر فاك أو
+
+459
+00:42:25,260 --> 00:42:28,460
+بنفصل الأمان إلى الأمان القريب، لكن إذا مش عارف
+
+460
+00:42:28,460 --> 00:42:33,060
+الأمان، بدك تبقى ديمين الإنسان عن .. أحيانا بلزم
+
+461
+00:42:33,060 --> 00:42:36,730
+بس يتسكر المفتاحبكون في مفتاح مثلا على الجنب
+
+462
+00:42:36,730 --> 00:42:41,850
+وبتطفو المفتاح خلاص بتفصل الكهربا أو أحيانا بظبطش
+
+463
+00:42:41,850 --> 00:42:46,530
+إلا نرفع البناء آدم إذا ماعرفناش ال .. وين الأمان
+
+464
+00:42:46,530 --> 00:42:50,710
+أو كان المفتاح مثلا مش مشبوك على الخط الراجع مشبوك
+
+465
+00:42:50,710 --> 00:42:55,530
+على خط الكهربا ماشي أحيان المفتاح مش مشبوك على
+
+466
+00:42:55,530 --> 00:43:02,240
+الخط الراجع فبنسوي الشغل هذاطبعا يعني كل .. بتاعين
+
+467
+00:43:02,240 --> 00:43:08,760
+الصدمات بدنا نخلي يتنفس و بدنا نعالج الخاطر ..
+
+468
+00:43:08,760 --> 00:43:14,460
+الخطورة اللي بتصير و نعمله تدليك للقلب إذا لزم و
+
+469
+00:43:14,460 --> 00:43:18,000
+بدنا إذا صار حروق نعالجها يعني الكهربا بتعمل حرق
+
+470
+00:43:18,000 --> 00:43:24,580
+أنا قولتلكوا إنه شوفت حالتين ناس من ال .. البرج
+
+471
+00:43:24,580 --> 00:43:29,540
+البرج عبارة عن شحنات كهربائيةعارفين كيف كان ..
+
+472
+00:43:29,540 --> 00:43:33,700
+باجوا يجيبوا الدكتورة الخصائي تبع التشريح بيسموه
+
+473
+00:43:33,700 --> 00:43:38,720
+الدكتورة التشريح من حد ما يطلع يقول هذا حارق كهربا
+
+474
+00:43:38,720 --> 00:43:45,880
+.. حارق غير .. حارق برج كيف حارق البرج؟ بيبقى ..
+
+475
+00:43:45,880 --> 00:43:53,140
+أو هذا كهربا برج وين الجسم ملتمس بالأرض دخول وخروج
+
+476
+00:43:53,140 --> 00:43:58,180
+الكهربا بيبينالجسم اللى واصل الأرض بيبقى فيه حروق
+
+477
+00:43:58,180 --> 00:44:07,080
+حرج عادي مكان دخول الكهربا كأنه فنان راسم ولد بلون
+
+478
+00:44:07,080 --> 00:44:11,900
+أحمر اخدين بالكوا كيف طلع على الجسم كأنه في واحد
+
+479
+00:44:11,900 --> 00:44:18,240
+فنان راسم لون أحمر دخول الكهربا خروجها عامل مكانه
+
+480
+00:44:18,240 --> 00:44:23,840
+حرج فإذا كان ضاله طيب بدي أنا أعالج الحرجماخدين
+
+481
+00:44:23,840 --> 00:44:28,100
+بالكوا كيف؟ في حروج بتنتج عن التيار الكهربي زيها
+
+482
+00:44:28,100 --> 00:44:33,840
+زي الحروج اللى بتنتج عن النار فبدى أعالج الحرق هذا
+
+483
+00:44:33,840 --> 00:44:38,540
+بتهيأح كنا مش هيك فاليوم صاروا إيش يعملوا في الخلة
+
+484
+00:44:38,540 --> 00:44:44,820
+أو في الدور مثلا العالية صاروا بيحطوا شيء للكهربا
+
+485
+00:44:44,820 --> 00:44:50,190
+مانع صواق يعني بيحطواعلى الأبراد على كل المناطق
+
+486
+00:44:50,190 --> 00:44:55,570
+العالية فكمان يعني ينصح ان اللي بيستخدم بيروح على
+
+487
+00:44:55,570 --> 00:44:59,190
+الخلة كتير و في برج و في رعد يكون فيه دوع صاية من
+
+488
+00:44:59,190 --> 00:45:04,840
+حديدعصار من حديد أو عصار من مادة معدم بحيث انه هذه
+
+489
+00:45:04,840 --> 00:45:10,320
+الصائق أسهله يمشي فيها الكهربا يعني اللي بتنزل من
+
+490
+00:45:10,320 --> 00:45:15,860
+ال .. من البرج أسهلها تمشي في الحديد من أنها تمشي
+
+491
+00:45:15,860 --> 00:45:20,540
+في الجسم فبتخفف هذه والأحسن ان لما يكون في صائق
+
+492
+00:45:20,540 --> 00:45:25,200
+نضبمافيش داعي نطلع في الشارع او نطلع هنا و هنا و
+
+493
+00:45:25,200 --> 00:45:28,960
+خاصة و انه في المطر بيبقى الواحد جسمه ملان ميه و
+
+494
+00:45:28,960 --> 00:45:37,400
+الميه موصل جيد للكهرباء خلصنا موضوع ايش الصدمة مين
+
+495
+00:45:37,400 --> 00:45:43,400
+عنده اي سؤال؟ اعراض الصدمة اعراض الصدمة ماحكيناهاش
+
+496
+00:45:43,400 --> 00:45:49,660
+ايش عندك مكتوب اعراض الصدمة جودي؟ اعراض الصدمة
+
+497
+00:45:49,660 --> 00:45:50,840
+يعني
+
+498
+00:45:53,710 --> 00:45:57,410
+في إعراض أول ما تصير الصدمة، إيش نتوقع يكون أول ما
+
+499
+00:45:57,410 --> 00:46:00,990
+تصير الصدمة؟
+
+500
+00:46:00,990 --> 00:46:07,550
+نحكيكوا إياها القصة بس نحكيكوا إياها القصة أمرك
+
+501
+00:46:07,550 --> 00:46:13,310
+روحت على مناسبة غير سعيدة واحدة أمها ماتت ولا
+
+502
+00:46:13,310 --> 00:46:18,910
+أخوها مات ولا إيش زي هيك جاب اليومين كيف بيكون
+
+503
+00:46:18,910 --> 00:46:21,750
+الأقرب للبني آدمها؟
+
+504
+00:46:26,380 --> 00:46:36,220
+ه؟ بكون شكله العام كيف؟ وجهه شاحب؟ و العرق في
+
+505
+00:46:36,220 --> 00:46:41,980
+جسمه؟ و مش هو يعني مثلا مثلا لو جينا نقول صدمة
+
+506
+00:46:41,980 --> 00:46:42,600
+عصبية
+
+507
+00:46:45,110 --> 00:46:51,590
+صدمة عصبية واحدة و هى بتدق في الشكوش ضربة أصبعها
+
+508
+00:46:51,590 --> 00:47:03,050
+بيصير ماكن ايش بيصير؟ ايش بيصير فيكى؟ مين
+
+509
+00:47:03,050 --> 00:47:09,770
+عمرها صارت معاها هيك و جامدة؟ ايش صار؟ كأنه بيغيب
+
+510
+00:47:09,770 --> 00:47:17,150
+الواحدكأنه بيفقد الوعي بحس الدنيا معتمة النظر
+
+511
+00:47:17,150 --> 00:47:27,170
+بيسير عليه كأنه run زوال جسمه بنهار ممكن يعرق و
+
+512
+00:47:27,170 --> 00:47:35,910
+بجعق هذه من الصدمة العصبية كمان الصدمة فقدان الدم
+
+513
+00:47:35,910 --> 00:47:43,070
+مثلا ممكن جدا برضهيخس قوته و يظل يتناقص القوة الى
+
+514
+00:47:43,070 --> 00:47:46,730
+انه يجعب و ينزل ضغطه و يصبح ضغطه واطى و يصبح نبضه
+
+515
+00:47:46,730 --> 00:47:54,170
+سريع كل هذه علامات من علامات الصدمة ايش كمان؟ اولا
+
+516
+00:47:54,170 --> 00:47:58,450
+يبدأ العقب تقضي الجسام ثمانا تقضي عقوبة الصدمة
+
+517
+00:47:58,450 --> 00:48:01,710
+تقريجيا اربع عيسى تقضي الصدمة سريعا و تقريجيا
+
+518
+00:48:01,710 --> 00:48:04,170
+وعادة منها تقضي نبض افضل
+
+519
+00:48:17,020 --> 00:48:27,520
+سمعى نعم نعم كله تقريبا حاكمة فكله تابع بعضه فأرض
+
+520
+00:48:27,520 --> 00:48:34,570
+الصدمة زي ما احنا حكينا و زي ما أختنا جالتالان
+
+521
+00:48:34,570 --> 00:48:41,730
+قولنا بدنا كيف نتعامل مع الصدمة بدنا نحسن كل إشي
+
+522
+00:48:41,730 --> 00:48:47,870
+بسبب خطأ الحاجة الأولى بدنا نحسن الدورة الدموية
+
+523
+00:48:47,870 --> 00:48:52,930
+كيف بدي أحسن الدورة الدموية؟ مين بتقولنا؟ بدي أرفع
+
+524
+00:48:52,930 --> 00:48:58,350
+رجلين إلى أعلى و بدي إيش أسوي؟ أسجي سوائد إذا
+
+525
+00:48:58,350 --> 00:49:01,730
+كانوا بيشرب أسجي سوائد طبعا اللي بتاعين الإسعاف
+
+526
+00:49:01,730 --> 00:49:08,260
+ممكن يركبوله دمو ممكن يركبوله محلول عشان إيش؟ يزيد
+
+527
+00:49:08,260 --> 00:49:14,440
+حجم الدم الحاجة التانية بده حسن التنفس كيف بده حسن
+
+528
+00:49:14,440 --> 00:49:19,880
+التنفس؟ في أحد الأيام .. في أحد الأيام في راجل غير
+
+529
+00:49:19,880 --> 00:49:27,960
+الحين مهندس كبير في البلد و مسؤول ولد صغير خش في
+
+530
+00:49:27,960 --> 00:49:33,930
+صدمة ظاهرة سبمية نتيجة التهيبات في جسمهفطبعا كان
+
+531
+00:49:33,930 --> 00:49:38,770
+إيش الناس بيساوي كانوا الناس زمان هذا الكلام يعني
+
+532
+00:49:38,770 --> 00:49:45,110
+من يمكن خمس و ثلاثين و أربعين سنة مافيش علم و أحنا
+
+533
+00:49:45,110 --> 00:49:51,110
+قلوبنا كلنا على بعض فأحيانا كتر الحنان أو كتر
+
+534
+00:49:51,110 --> 00:49:57,230
+المحب أو كتر الشفقة بتخرب كتر الحنان و الاهتمام
+
+535
+00:49:57,230 --> 00:50:04,150
+بخرب فالولد أيه طبعا كل نسوان الحارةأجى نلتف
+
+536
+00:50:04,150 --> 00:50:09,890
+حوالين الولد وصار نزاجا ويلطمن يا خست يا حرام
+
+537
+00:50:09,890 --> 00:50:13,370
+مسكين و الله يخسر عليه و الله شاطر و الله .. و
+
+538
+00:50:13,370 --> 00:50:16,810
+يصيروا عادي يقولوا في المناقب الحميدة و الصفات
+
+539
+00:50:16,810 --> 00:50:22,050
+الطيبة لهذا البنادم ايش جاعدات بيساون هدول؟ جاعدات
+
+540
+00:50:22,050 --> 00:50:28,150
+كلهين بتنفسوا خنجنهمظبوط ولا لا؟ إلا إن ربنا بعت
+
+541
+00:50:28,150 --> 00:50:32,590
+واحد هيك فتحة نتفى وشاطرة حملته من بينهم وودته في
+
+542
+00:50:32,590 --> 00:50:37,910
+جاع الدار أو في الحوش وحمله ووده على المستشفى فأنا
+
+543
+00:50:37,910 --> 00:50:44,170
+عشان أحسن التنفس إذا عندي سطوانة أكسوجين بدي أعطيه
+
+544
+00:50:44,170 --> 00:50:47,390
+أكسوجين مافيش عنده أكسوجين بدي أخليه يتنفس هو نظيف
+
+545
+00:50:47,390 --> 00:50:52,010
+يعني مانفعش واحد مخنوق أحطه في غرف زي هيك مغلقة
+
+546
+00:50:52,010 --> 00:50:58,810
+بفتح الشبابيك بفتح الهوياتبطلع برا بابعد الناس
+
+547
+00:50:58,810 --> 00:51:02,170
+اللي حواليه لإنه ممكن جدا قال خاله و عمه و كذا
+
+548
+00:51:02,170 --> 00:51:05,650
+ييجي من كتر ما هو زعلان اللي هو السجارة من أختها
+
+549
+00:51:05,650 --> 00:51:10,910
+يولعها و يدخن كان زمان في حاجة يقولك ولع نار عشان
+
+550
+00:51:10,910 --> 00:51:17,690
+يعرقعشان ينخنج مش عشان يهرج عشان يموت فهذا كلام
+
+551
+00:51:17,690 --> 00:51:22,450
+كله عشان نحسن الدورة الدموية بدنا نرفع أجريه عشان
+
+552
+00:51:22,450 --> 00:51:25,930
+نحسن ال oxygen إذا عندي سوانة oxygen بدي أعطيه
+
+553
+00:51:25,930 --> 00:51:30,050
+منها علشان حسن ال oxygen بدي أفتح الشبابيك و
+
+554
+00:51:30,050 --> 00:51:34,290
+البواب طبعا أنا متصل في الإسعاف و بدي جيني بس في
+
+555
+00:51:34,290 --> 00:51:37,990
+فترة ما يصلني الإسعافبدي اكون عامل الإصحافات اللي
+
+556
+00:51:37,990 --> 00:51:43,190
+انا لازم اعملها حرارته بدي احافظ عليها بحيث انه
+
+557
+00:51:43,190 --> 00:51:49,110
+ايش ماتزيدش ولا تخس لان انزادت هتعمل مشكلة كمان
+
+558
+00:51:49,110 --> 00:51:54,770
+اذا خست هتعمل مشكلة
+
+559
+00:51:54,770 --> 00:52:00,410
+في
+
+560
+00:52:00,410 --> 00:52:02,710
+عنا شوية تحذيرات هنا اللي هي ايش
+
+561
+00:52:06,230 --> 00:52:11,790
+الصدمات تسبب حاجة متزيلة للأكسجين لابد من السيطرة
+
+562
+00:52:11,790 --> 00:52:14,670
+على النزيف إذا كان هناك أصابات في الرأس ما نحركش
+
+563
+00:52:14,670 --> 00:52:20,150
+حماية المصاب من أي أصابات أخرى واتصال بسيارة
+
+564
+00:52:20,150 --> 00:52:23,610
+الإسعاف دايما .. دايما .. دايما الله مكرمنا
+
+565
+00:52:23,610 --> 00:52:28,370
+الجوالات تحت إيدنا ماكانش إلك بكون كل واحد ماري
+
+566
+00:52:28,370 --> 00:52:33,340
+حواليكي كان ذكر أو أنثى غالبا مع جوالوشاطرين
+
+567
+00:52:33,340 --> 00:52:37,860
+الحكومة عاملين كل المؤسسات اللي هى مبلاش سواء
+
+568
+00:52:37,860 --> 00:52:41,600
+ماعاكى كارت أو ماعاكيش كارت بتتصل ع 101 كم رقم
+
+569
+00:52:41,600 --> 00:52:48,060
+الإسعاف؟ 101 رقم الشرطة؟ 102 رقم الشرطة؟ 100؟ اه
+
+570
+00:52:48,060 --> 00:52:54,300
+الدفاع المدني .. الدفاع المدني 102 فبنتصل بالظبط
+
+571
+00:52:54,300 --> 00:53:04,200
+على الإنسان و المعنى و بنخلي يجي يعملنا اللازمطبعا
+
+572
+00:53:04,200 --> 00:53:07,880
+يعني لو الصدمة العصبية انتوا بتشوفوها يمكن في
+
+573
+00:53:07,880 --> 00:53:13,760
+الامتحانات عمركوا شوفت واحدة في الامتحان سخسخت من
+
+574
+00:53:13,760 --> 00:53:17,960
+بارح بنت ابني بتقدم توجيهي انا سيدها و بتقدم
+
+575
+00:53:17,960 --> 00:53:24,220
+توجيهي تصوروا نعم لأ هي أدبي التارية تارية جالت يا
+
+576
+00:53:24,220 --> 00:53:31,440
+سيدها واحدة صارت تجمد وبعتملي أمي وين أمي واحدة
+
+577
+00:53:31,440 --> 00:53:32,900
+فطت من الشباك
+
+578
+00:53:35,610 --> 00:53:41,370
+ه؟ سمعتيها؟ هاي أخت سامع، واحدة فطت من الشباك
+
+579
+00:53:41,370 --> 00:53:45,650
+والتاني صارت تقول جيبولي أمي، مااخدين بالك؟ فهذه
+
+580
+00:53:45,650 --> 00:53:53,050
+عبارة عن إيش؟ يعني الجثة صدمة عصبية و لا لأ؟
+
+581
+00:53:53,050 --> 00:54:01,880
+فالصدمة العصبية هذه بدها يعني هدوءبدها تعالج
+
+582
+00:54:01,880 --> 00:54:09,960
+بطريقة انه يعني نحافظ على التنفس بالظبط
+
+583
+00:54:09,960 --> 00:54:13,860
+و هذا بيصير مع الصبايا يعني ممكن يسكر الأسنسير تطف
+
+584
+00:54:13,860 --> 00:54:18,520
+الكهربا و انت في الداخل بت .. بتنهزي .. بتنهزي و
+
+585
+00:54:18,520 --> 00:54:25,680
+بيصير عندك شوية مشاكل مين عندها اي سؤال؟ مين عندها
+
+586
+00:54:25,680 --> 00:54:30,470
+اي سؤال يا خوتي؟ هيك تمام؟هك ان شاء الله بنكون
+
+587
+00:54:30,470 --> 00:54:35,590
+خلصنا من موضوع الصدمة في عندنا حاجة تانية اسمها
+
+588
+00:54:35,590 --> 00:54:43,590
+إيش الغيبوبة الغيبوبة إيش بتفرج عن الصدمة؟ إيش
+
+589
+00:54:43,590 --> 00:54:51,270
+بتفرج الغيبوبة عن الصدمة؟ مين عارفها؟
+
+590
+00:54:51,270 --> 00:54:57,530
+الغيبوبة مش كتير لعلاقة بالدم إنما لعلاقة بإيش؟
+
+591
+00:55:00,260 --> 00:55:05,700
+بالجهاز العصبي الإغماق بسيبه بساوي غيبوبة بساوي
+
+592
+00:55:05,700 --> 00:55:11,160
+غيبوبة ممكن
+
+593
+00:55:11,160 --> 00:55:15,400
+لو اتأخر في الصدمة يفقد وعي لو مالحجنهوش وعلجناه
+
+594
+00:55:15,400 --> 00:55:22,020
+يفقد وعي الغيبوبة هي عبارة عن فقدان
+
+595
+00:55:22,020 --> 00:55:30,590
+الوعي وعدم إدراك الشخص بما يدور حولهواحد بيبقى مش
+
+596
+00:55:30,590 --> 00:55:35,490
+صاحي إيش بيصير حواليه و بفقد وعيه زي ما حكينا إلكم
+
+597
+00:55:35,490 --> 00:55:41,010
+ممكن بنت تبقى تقدم امتحان تفتح أول سؤال تطلمس
+
+598
+00:55:41,010 --> 00:55:46,990
+عينيها تبطل تشوف اللي هي مغايبة مجرد هذه البنت ما
+
+599
+00:55:46,990 --> 00:55:53,370
+تنام على الأرض بتروك مجرد ما تنام على الأرض بتروك
+
+600
+00:55:53,370 --> 00:56:00,170
+إيش اللي بيصير معها؟ لما تنام على الأرضالمخ مع
+
+601
+00:56:00,170 --> 00:56:07,070
+الضغط بطل يصل المخ الدم الكفاية لما نعمت على الأرض
+
+602
+00:56:07,070 --> 00:56:19,190
+صار عندنا ايش ما يسمى بالصحصح او كده فاسباب
+
+603
+00:56:19,190 --> 00:56:23,010
+الغيموبة يا أخوة المجدات التهابات او مشاكل في
+
+604
+00:56:23,010 --> 00:56:27,560
+الجهاز العصبيإذا الجهاز العصبي اللي هو عبارة عن
+
+605
+00:56:27,560 --> 00:56:36,060
+المخ اللي هو يعتبر المسؤول الأول و الأهم عن كل
+
+606
+00:56:36,060 --> 00:56:42,540
+الجسم إلا الجلب صح؟ عشان يقولنا من الذاكرات أن
+
+607
+00:56:42,540 --> 00:56:46,120
+موجودة أنواع العضلات و أن في إيرادية و لا إيرادية
+
+608
+00:56:46,120 --> 00:56:53,130
+و في عضلات الجلب ليش حطوا الجلب لحاله؟لأن عضلة
+
+609
+00:56:53,130 --> 00:56:59,870
+القلب لا إرادية ولكن لا ترتبط بالجهاز العصبي يعني
+
+610
+00:56:59,870 --> 00:57:05,270
+ممكن يموت الواحد ويظل جلبه شغال لفترة ويظل جلبه
+
+611
+00:57:05,270 --> 00:57:13,490
+شغال لفترة وبناء عليه عضلة القلب يعني برا اللي بقى
+
+612
+00:57:13,490 --> 00:57:17,770
+فالجهاز العصبي مسئول عن كل إشي في الجسم مع دل جلب
+
+613
+00:57:17,770 --> 00:57:22,560
+أحيانا هذا الجهاز العصبي بيصير فيه خلدبصير فيه
+
+614
+00:57:22,560 --> 00:57:27,160
+مشاكل من المشاكل اللي بصير فيه إيش إصابات الرأس
+
+615
+00:57:27,160 --> 00:57:36,240
+كيف بده يصير إصابة في الرأس؟ واحد بلعب كورة وقعد
+
+616
+00:57:36,240 --> 00:57:42,140
+له خابط رأسه في الأرض بخش في غيبوبة ممكن أنا أعرف
+
+617
+00:57:42,140 --> 00:57:51,160
+طفل في الشجاعية كان نايم في مستشفى الوفع للأسف
+
+618
+00:57:51,930 --> 00:57:58,210
+اه فهذا الطفل يعني كنت ماخد طلاب معايا و رايح كان
+
+619
+00:57:58,210 --> 00:58:03,450
+الولد له حوالي شهرين مغيب فبسأل ايش القصة قالوا
+
+620
+00:58:03,450 --> 00:58:08,130
+بلعب في طيارة اللي بيطيروا فيها الأطفال ماشي و هو
+
+621
+00:58:08,130 --> 00:58:14,510
+ماشي زي هيك حجر دجم في رجله وقع على رأسه أجى على
+
+622
+00:58:14,510 --> 00:58:19,210
+المخيخ كان أيام ما شوفته أكتر من شهرين اللي هو
+
+623
+00:58:19,210 --> 00:58:25,560
+مغيبمن إصابة في الرأس ايش فقد من دمه ولا حاجة
+
+624
+00:58:25,560 --> 00:58:29,400
+بتروح على المستشفى ايش مال فلان كان قالك اندر
+
+625
+00:58:29,400 --> 00:58:33,900
+ارتجاج في المخ ايش كان ارتجاج في المخ ولا نقطة دم
+
+626
+00:58:33,900 --> 00:58:42,680
+رايحة منه زاد الدم في حركة في المخ غير طبيعية صارت
+
+627
+00:58:42,680 --> 00:58:46,540
+لكن مافقد ولا نقطة دم ولا صار فيه اي حاجة فإصابات
+
+628
+00:58:46,540 --> 00:58:50,320
+المخ هي المسؤولة عن ما يسمى
+
+629
+00:58:53,030 --> 00:59:00,550
+بالغيبوبة ف في الرياضة في الملاكمة في حوادث الطرق
+
+630
+00:59:00,550 --> 00:59:06,810
+في كسر الجمجمة سواء داخلي او خارجي في أمراض الجهاز
+
+631
+00:59:06,810 --> 00:59:12,470
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+
+632
+00:59:12,470 --> 00:59:17,030
+يغيب عمرك شفته واحد صار عنده جلطة في الدماغ شفته
+
+633
+00:59:17,030 --> 00:59:24,220
+اه كتيرفهذا مرض يعني شائع عليها نعم لما يصير جلطة
+
+634
+00:59:24,220 --> 00:59:29,320
+في الدماغ كدهش البني آدم بيخسر دم ولا شيء انما ايش
+
+635
+00:59:29,320 --> 00:59:34,080
+اللي بتأثر الجهاز العصبي فالجهاز العصبي هو اللي
+
+636
+00:59:34,080 --> 00:59:40,540
+بتأثر بيصير عند البني آدم غيبوبة كمان الحمّة
+
+637
+00:59:40,540 --> 00:59:47,680
+الشوكية سمعتوا فيها اللي هي التهاب السحاية أغشية
+
+638
+00:59:47,680 --> 00:59:52,730
+المخالاتهاب اغشية المخ اغشية المخ هذه مغطية او
+
+639
+00:59:52,730 --> 00:59:58,430
+مغطية كل شي في الجهاز العصب مغطية المخ ومغطية
+
+640
+00:59:58,430 --> 01:00:04,410
+النقاء الشوكي كله فلما يصير فيها اتهاب بتأثر المخ
+
+641
+01:00:04,410 --> 01:00:12,270
+وبتأثر الجسم هذه برضه في وجتها يعني ممكن تعمل
+
+642
+01:00:12,270 --> 01:00:18,300
+مشاكل و تضرب المخ جديش خص الدمفيش إشي خاص إنما
+
+643
+01:00:18,300 --> 01:00:23,280
+ممكن تعمل مشاكل أنا بعرف ناس كتير كانوا بيصيبوا
+
+644
+01:00:23,280 --> 01:00:26,960
+بالحمة الشوكية هي غالبا بيصيب الأطفال بس ممكن تصيب
+
+645
+01:00:26,960 --> 01:00:33,460
+كمان الكبار من صابوا بالحمة الشوكية إذا ما جابوهوش
+
+646
+01:00:33,460 --> 01:00:39,080
+بسرعة على المستشفى كثير منهم بيصير عندهم مضاعفات
+
+647
+01:00:39,080 --> 01:00:44,980
+يعني بطيب الولد أو البنت و عنده شلب بطيب الولد و
+
+648
+01:00:44,980 --> 01:00:52,070
+عنده تخلف عقليمش حرارة زايدة هاد التهاب الحمّة
+
+649
+01:00:52,070 --> 01:00:55,990
+الشوكية سببها
+
+650
+01:00:55,990 --> 01:01:01,890
+انه ال microbe أعراضها بدّاك حكيناها احنا احنا
+
+651
+01:01:01,890 --> 01:01:05,490
+حكينا أعراضها قولنا انه ماحكيناش أعراض الحمّة
+
+652
+01:01:05,490 --> 01:01:10,630
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+
+653
+01:01:10,630 --> 01:01:18,480
+أعراضها تذكروا أيام أجت السلطة زمانصار فيه انتشار
+
+654
+01:01:18,480 --> 01:01:25,840
+حمّ شوقي في البلد بشكل فظيع نعم صار micro-opted
+
+655
+01:01:25,840 --> 01:01:34,720
+بالبلد صار
+
+656
+01:01:34,720 --> 01:01:39,480
+فيه التهاب يعني واضح فصار الناس خايفة يعني يجيبون
+
+657
+01:01:39,480 --> 01:01:44,020
+أولادهم يشوف إنها الولد كنت أعمل حركة واحدة فقط
+
+658
+01:01:44,020 --> 01:01:50,830
+للي بيجيناكنت انام و احط ايدي تحت راسه مش تحت
+
+659
+01:01:50,830 --> 01:01:58,310
+رقبته و ارفع اذا ارتفع قدمت راسه و ارتفع اقول له
+
+660
+01:01:58,310 --> 01:02:03,250
+مافيش فيه حاجةإذا طلع شجف واحد كأنك حط خشبة بين
+
+661
+01:02:03,250 --> 01:02:09,550
+الراس والظهر يعني هادي تصير شجفة تشنج هقولهم
+
+662
+01:02:09,550 --> 01:02:14,810
+إلحقوا ان درجة أختي في الليل حوالي ممكن بقيت عشرة
+
+663
+01:02:14,810 --> 01:02:18,770
+في الليل وادواجت متأخرت تصلت عليا جالتلي أخوي
+
+664
+01:02:18,770 --> 01:02:23,910
+بقولها يا أختي جالت الولاد جيراننا التنين وادوهم
+
+665
+01:02:23,910 --> 01:02:26,930
+على المستشفى وطلع عندهم حمشاوكية وابنى والله
+
+666
+01:02:26,930 --> 01:02:32,550
+شايفها مش مبسوطأنا خايف فيه إشي قولتلى أختي اعمل
+
+667
+01:02:32,550 --> 01:02:36,270
+هالحركة نايميه و احط يدك تحت رأسي و ارفعيها إذا
+
+668
+01:02:36,270 --> 01:02:40,470
+انتنت رجبتي و تجلجيش و فعلا عملتها قالتلى لأ انت
+
+669
+01:02:40,470 --> 01:02:44,630
+انت مافيش مشكلة و روحتلى جيتش مشكلة صحية فأول
+
+670
+01:02:44,630 --> 01:02:53,150
+أعراضها إيش تشنج عضلات الرجب تشنج أو تسلب عضلات
+
+671
+01:02:53,150 --> 01:02:59,120
+الرجب زى التتانوس أول أعراضه إيش شد عضلاتالفك
+
+672
+01:02:59,120 --> 01:03:03,600
+ماخدين بالك و كيف فهذه من عراض الحم الشوكية أخوات
+
+673
+01:03:03,600 --> 01:03:07,900
+الماجدات عشان تمنع المضاعفات لو لقيت حاجة زي هيك
+
+674
+01:03:07,900 --> 01:03:13,240
+اعطي الطفل او اللي عنده هذه العراض اعطيه جرعة مضاد
+
+675
+01:03:13,240 --> 01:03:19,860
+حيوي مجوزة ايش يعني مجوزة double يعني احنا بنعطيه
+
+676
+01:03:19,860 --> 01:03:25,080
+معلقة جرعته معلقة صغيرة بنعطيه معلقتين بقولوا ان
+
+677
+01:03:25,080 --> 01:03:29,690
+هذه الجرعة بتمنع المضاعفاتيعني بيصيرش ان شاء الله
+
+678
+01:03:29,690 --> 01:03:35,790
+بيصيرش لا شلل ولا بيصير فقدان شيء في الجهاز العصبي
+
+679
+01:03:35,790 --> 01:03:41,210
+لا سمع ولا بصر ولا .. ولا تخلف ولا أي حاجة و بعدين
+
+680
+01:03:41,210 --> 01:03:46,050
+بنوديه بسرعة على أقرب مركز طبي و بنقولهم أنه أعطنا
+
+681
+01:03:46,050 --> 01:03:52,610
+جرعة مضاد حيوي كبيرة لأنه بيعملوله مزرعة و لو ممكن
+
+682
+01:03:52,610 --> 01:03:53,650
+المضاد الحيوي
+
+683
+01:03:56,730 --> 01:04:01,590
+مزرعة بياخد من ظهره بازل ظهر بيحطوا إبر في الظهر
+
+684
+01:04:01,590 --> 01:04:06,130
+تحت النخاع الشوكي بيطلع بيحطوا في أنبوب و بيبعتوا
+
+685
+01:04:06,130 --> 01:04:11,570
+على المقتبر بيلاقوا فيها إيش بكتيريا أو بيلاقوا
+
+686
+01:04:11,570 --> 01:04:15,950
+فيها مشاكل سكر و حاجات زي هيك بتدل عندهم طبعا
+
+687
+01:04:15,950 --> 01:04:21,270
+عارفين كيف بينفحص و بيقولوا أنه في حم شوكية و لا
+
+688
+01:04:21,270 --> 01:04:25,040
+فيش حم شوكيةطبعا انت قولي للطبيب المعالج انه
+
+689
+01:04:25,040 --> 01:04:29,300
+اعطيناه مضاعف حيوي لان هذه بتخفف من ظهور البكتيريا
+
+690
+01:04:29,300 --> 01:04:35,420
+في المزرعة لكن اعطيه عشان تمنعي حدوث اي مضاعفات
+
+691
+01:04:35,420 --> 01:04:42,960
+ناشي؟ فاذا الغيبوبة هذه اللي علاقة بالجهاز العصبي
+
+692
+01:04:42,960 --> 01:04:49,460
+زي التهابات، المخ، انسداد الشرايين، حمش شوقية، في
+
+693
+01:04:49,460 --> 01:04:55,310
+حاجة اسمها السرعفيه صراع الاب لبسي الصراع فيه كبير
+
+694
+01:04:55,310 --> 01:05:00,990
+وفيه صغير ايش يعني الصراع الصراع
+
+695
+01:05:00,990 --> 01:05:09,330
+هو مرض غير معروف للأسباب حتى اليوم اسمعته النبي
+
+696
+01:05:09,330 --> 01:05:13,830
+صلى الله عليه وسلم جاته امرأة تقول يا رسول الله
+
+697
+01:05:13,830 --> 01:05:19,990
+اني أسرة ايش قاله له النبي مين ذاكر ايش النبي قال
+
+698
+01:05:22,220 --> 01:05:30,140
+قال إن شئتي دعوت لكي فشفاك الله وإن شئتي صبرتي
+
+699
+01:05:30,140 --> 01:05:36,820
+ولكي الجنة وإن شئتي ..سمعتوش القصات؟ غريب، كتير
+
+700
+01:05:36,820 --> 01:05:40,840
+بتنجدها تبتسمع فطبعا واحد عاجل زي حكايتكوا يعني
+
+701
+01:05:40,840 --> 01:05:47,910
+إيش بختار؟ قطعونيو اروح الجنة اللى بيستشهدوا و
+
+702
+01:05:47,910 --> 01:05:51,410
+اللى بيخشوا معارك و اللى بيطلعوا و بنزلوا و كده
+
+703
+01:05:51,410 --> 01:05:57,930
+لليه؟ ايش الهدف؟ الجنة يا رسول الله ليسوا بينى و
+
+704
+01:05:57,930 --> 01:06:01,590
+بين الجنة الا ان انا اكل هذه التمرات قال نعم قال
+
+705
+01:06:01,590 --> 01:06:06,110
+ها انها لحياة طويلة ارماها و دخل في المعاركة و قبل
+
+706
+01:06:06,110 --> 01:06:11,510
+بلاء حسن عشان اروح وين؟ الجنة فالنبي فش يمر عنى
+
+707
+01:06:11,510 --> 01:06:18,800
+مطلقاإنه قال لأحد مريض إن شئت صبرت ولك الجنة وإن
+
+708
+01:06:18,800 --> 01:06:27,980
+شئت دعوت لك إلا هذا لأن النبي وما ينطقه إن هو إلا
+
+709
+01:06:27,980 --> 01:06:34,300
+واحد من يوحى فالصراء لحتى يومنا هذا ليس له علاج
+
+710
+01:06:34,300 --> 01:06:40,890
+شافي ليس له علاج شافي طب أيش اللي بيعملوهاللي
+
+711
+01:06:40,890 --> 01:06:47,510
+بيعملوه بيعطوا علاج بيعطوا علاج بيعمل حاجتين
+
+712
+01:06:47,510 --> 01:06:54,210
+الحاجة الأولى بتيجي بأقل حدة النوبة بتيجي بأقل حدة
+
+713
+01:06:54,210 --> 01:06:59,850
+الحاجة التانية بيبعد مدة النوبة عن أختها يعني أنا
+
+714
+01:06:59,850 --> 01:07:04,230
+خرفوني أنه في طفل بيصرع في اليوم حوالي عشرين مرة
+
+715
+01:07:04,230 --> 01:07:08,850
+في اليوم هذا لو صار يصرع مرة في اليوم إيش بيقولوا
+
+716
+01:07:08,850 --> 01:07:15,980
+أهله؟اتحسن ولا لأ؟ بيقولوا اتحسن واحد يصرع في
+
+717
+01:07:15,980 --> 01:07:19,680
+الشهر مرة لو صار كل سنة مرة ايش بيقولوا اهله طار
+
+718
+01:07:19,680 --> 01:07:27,940
+طار واحد يصرع بأنف وشد وكذا يسير يجيب هدوء بيقولوا
+
+719
+01:07:27,940 --> 01:07:32,500
+طار فاحنا اللي بيعملوه اليوم بيعطوا علاج يخفف حدة
+
+720
+01:07:32,500 --> 01:07:39,570
+الصراع ويبعد المدةو يبعد المدى مافيش لحد اليوم دوة
+
+721
+01:07:39,570 --> 01:07:46,050
+شافة مية في المية طبعا هم بيقولوا انه بيعملوا فحص
+
+722
+01:07:46,050 --> 01:07:53,490
+للمخ رسم كهربي للمخ هم بيطلع عند معظمهم شحنات
+
+723
+01:07:53,490 --> 01:07:58,030
+كهربائية بتشنج؟
+
+724
+01:07:58,030 --> 01:08:00,830
+ليش بيسوي الصراع؟ مين عمره شافت واحد بتشنج، بتسقى؟
+
+725
+01:08:00,830 --> 01:08:02,490
+كيف؟ كيف؟
+
+726
+01:08:05,400 --> 01:08:10,120
+بتشنّر كل جسم بيصير تشنّر ماخدين بالكوكب و بيفقد
+
+727
+01:08:10,120 --> 01:08:15,000
+الوعي بيفقد الوعي كمان طبعا هذا الصراع الكبير
+
+728
+01:08:15,000 --> 01:08:19,540
+فمعظمهم
+
+729
+01:08:19,540 --> 01:08:23,080
+بيطلع عنده زيادة كهرب في المخ بس قد يطلع مافيش
+
+730
+01:08:23,080 --> 01:08:27,420
+عنده كهرب خفيف في المخ و يصرع و قد يكون عند بعض
+
+731
+01:08:27,420 --> 01:08:30,920
+الناس كهرب زيادة في المخ و مايصرعش عشان هيك النبي
+
+732
+01:08:30,920 --> 01:08:35,720
+صلى الله عليه و سلم قال لنا إن شئتي دعوت لكيفشفاك
+
+733
+01:08:35,720 --> 01:08:39,140
+الله وإن شئت صبرتي ولك الجنة قالت يا رسول الله إني
+
+734
+01:08:39,140 --> 01:08:44,940
+أتكشف فاضغ الله ليه قال أتكشف فدعلها صارت لا تصرع
+
+735
+01:08:44,940 --> 01:08:48,500
+إلا و هي في الصلاة أو و هي ساجدة مرت عن كل قصة بس
+
+736
+01:08:48,500 --> 01:08:56,920
+انت نسيت يمكن نعم نعم فضربات في الجهاز العصبي ممكن
+
+737
+01:08:56,920 --> 01:09:04,630
+تعمل ما يسمى بالغيبوبة انسداد الشرايينزي ما قلنا
+
+738
+01:09:04,630 --> 01:09:08,830
+في النزيف او في الجلطة في عندنا حاجة اسمها غيبوبة
+
+739
+01:09:08,830 --> 01:09:15,050
+زياد السكر و غيبوبة نقص السكر في ناس بيصير عندهم
+
+740
+01:09:15,050 --> 01:09:20,770
+سكر زيادة و في ناس بيصير عندهم سكر ناقص طب كيف
+
+741
+01:09:20,770 --> 01:09:24,990
+بتعرف افرق بين سكر زيادة و سكر ناقص؟ مين عنده حد
+
+742
+01:09:24,990 --> 01:09:31,630
+معاه سكرين؟ بتسويش
+
+743
+01:09:31,630 --> 01:09:33,310
+السكر زيادة هذه التهبات؟
+
+744
+01:09:35,670 --> 01:09:41,690
+السكر ازيانى يا أخوات الماجدات بيصير رائحة لنفس
+
+745
+01:09:41,690 --> 01:09:46,270
+المصاب زى ريحت الاسيتون زى ريحت الاسيتون اللى
+
+746
+01:09:46,270 --> 01:09:50,190
+بتجيبه فى المناكير أو زى ريحت التفاح التالف و
+
+747
+01:09:50,190 --> 01:09:56,370
+بيكون حالة سكر يعنى معروفة و بتحماش باوكل اشمجا
+
+748
+01:09:56,370 --> 01:10:01,370
+طبعا هذه يعني قد يكون فى خطأ من الطاقة من الطب
+
+749
+01:10:01,370 --> 01:10:10,010
+اللى بتعامل معاهانا بعرف ناس عنده
+
+750
+01:10:10,010 --> 01:10:15,370
+سكر فالراجل هذا زى ما تقوله لما يقول لو واحد عندك
+
+751
+01:10:15,370 --> 01:10:18,630
+سكر يبقى بتلاقيه بالتزم مية في المية في أولها
+
+752
+01:10:18,630 --> 01:10:22,910
+بياخد علاجه مية في المية بتحمى مية في المية في
+
+753
+01:10:22,910 --> 01:10:27,930
+أولها فبعد ما اتحمى و أخد علاجه و إش زى هيك بعد
+
+754
+01:10:27,930 --> 01:10:34,320
+تلت أربعة شهور راح يفحص عند حد برااللي هو جاي بقول
+
+755
+01:10:34,320 --> 01:10:39,320
+شايف قالولي مين الحمار اللي قالك عندك سكري السكري
+
+756
+01:10:39,320 --> 01:10:45,930
+طلع عندى 100% مافيش سكري طبعا هو كلامه غلطفي علاج
+
+757
+01:10:45,930 --> 01:10:49,310
+مياخد وفي تحمية احنا ليش بنعطي علاج للبناء آدم
+
+758
+01:10:49,310 --> 01:10:53,830
+عشان نرجعوا للطبيعي أو لقريب من الطبيعي ولا مش هيك
+
+759
+01:10:53,830 --> 01:10:58,390
+عندك حرارة عالية بنعطيكي علاج للحرارة عشان نرجع
+
+760
+01:10:58,390 --> 01:11:01,630
+حرارتك للطبيعي انجيز حرارة و هي طبيعية نقول مين
+
+761
+01:11:01,630 --> 01:11:05,550
+الحمار اللي قال عندكي إيش حرارة طب وقف علاجك مثلا
+
+762
+01:11:05,550 --> 01:11:11,230
+بترجع تاني و يمكن أسوأ فبرجع مسكين بيوكل أكل كله
+
+763
+01:11:11,230 --> 01:11:15,890
+حلو و بده يعوض الحرمان اللي انحرموهالمد اللى فاتت
+
+764
+01:11:15,890 --> 01:11:22,410
+فبيخش بما يسمى بزيادة السكر غيبوت زيادة السكر كيف
+
+765
+01:11:22,410 --> 01:11:24,990
+بدنا نعرف يقولنا انه بيصير غيبوت زيادة السكر بيصير
+
+766
+01:11:24,990 --> 01:11:31,070
+فيه ريحة زى ريحة التفاح التالف او زى ريحة الاسيتول
+
+767
+01:11:31,070 --> 01:11:38,670
+ماشي هدول طبعا يعني بكل بساطة مشاكلهم اقل من مشاكل
+
+768
+01:11:38,670 --> 01:11:45,930
+اللى بيصير عنده غيبوت نقص السكر طب ليش اجلمين
+
+769
+01:11:45,930 --> 01:11:52,530
+يقول؟ مين يقول؟ ليش أجل؟ غيبوبة نقص السكر أشد
+
+770
+01:11:52,530 --> 01:11:57,850
+خطورة، ليش؟ بالظبط،
+
+771
+01:11:57,850 --> 01:12:02,610
+المخ بيتغذى على السكر، المخ والجهاز العصبي بتغذى
+
+772
+01:12:02,610 --> 01:12:06,610
+على السكر، الخلايا في جسمنا كلها بتتغذى على السكر،
+
+773
+01:12:06,610 --> 01:12:11,330
+فلما يوصل الغيبوبة معناته بصلش سكر للمخماخدين
+
+774
+01:12:11,330 --> 01:12:16,210
+بالكوا كيف فلو طولنا شوية هيبدأ خلايا المخ تتلف
+
+775
+01:12:16,210 --> 01:12:22,130
+تبدأ خلايا المخ تتلف بيصير مشكلة دائمة عند الإنسان
+
+776
+01:12:22,130 --> 01:12:27,270
+بيصير خلال دائم عند الإنسان وممكن يموت فعشان هيك
+
+777
+01:12:27,270 --> 01:12:31,930
+يا أخوات المجيدات كيف بدأ أعرف ان هذا الإنسان عنده
+
+778
+01:12:31,930 --> 01:12:37,550
+زيادة السكر او نخس السكر طبعا نخس السكر يكون
+
+779
+01:12:37,550 --> 01:12:44,650
+الإنسان مضطرببكون إنسان يتعالج للسكري و ملتزم جدا
+
+780
+01:12:44,650 --> 01:12:50,990
+بالحمية و باخد العلاج 100% لكن مش في وقته كيف؟
+
+781
+01:12:50,990 --> 01:12:56,410
+باخده قبلأول مرة و تاني مرة و تالت مرة بياخده قبل
+
+782
+01:12:56,410 --> 01:13:00,130
+قبل قبل بيظل ياخد من سكره من سكره من سكره إلى أنه
+
+783
+01:13:00,130 --> 01:13:04,990
+سكره ينتهي فبصير ياخد من السكر اللي أقل من الطبيعي
+
+784
+01:13:04,990 --> 01:13:10,970
+بنزل لوضع بيصير وضعه صعب جدا و بتأثر مخه و بيصير
+
+785
+01:13:10,970 --> 01:13:19,990
+مشكلة كيف بتعرف؟ قولنا بيبقى مضطرب عرجان عينية
+
+786
+01:13:19,990 --> 01:13:28,100
+متسعة ماخدين بالكوا كيف؟و مش مركز هذا بكون عنده ما
+
+787
+01:13:28,100 --> 01:13:34,200
+يسمى بغيبوبة او نقص السكر ايش علاجه مادول الناس؟
+
+788
+01:13:34,200 --> 01:13:39,480
+بدي اعطيه حاجة حلوة اذا ببلع اذا ببلع بدي اعطيه
+
+789
+01:13:39,480 --> 01:13:44,840
+شيء حلو عشان ارفع السكر عشان هيك احنا بننصح تابعين
+
+790
+01:13:44,840 --> 01:13:49,140
+السكر انه دايما دايما اللي بخده علاجات هذه يكون
+
+791
+01:13:49,140 --> 01:13:54,390
+معاهم اشي حلوبرح و اللي هو اول كان عندنا جارنا و
+
+792
+01:13:54,390 --> 01:13:57,890
+بقولي والله انا السكر بنحرق عندي فبقولي الزلمة
+
+793
+01:13:57,890 --> 01:14:00,510
+خلّي معاك دايما حاجة حلوة قال انا وانا كده بقدر
+
+794
+01:14:00,510 --> 01:14:05,430
+أحملها بقولي راضي الحبط حلو احنا بنحمل كيلوس سكر؟
+
+795
+01:14:05,430 --> 01:14:09,250
+الزلمة فاكر انك بتحمل كيلوس سكر حبط حلو او حبتين
+
+796
+01:14:09,250 --> 01:14:13,650
+حلو حطهم في جيبتك ومشي فيهم عادي لو حسيت اي ايش
+
+797
+01:14:13,650 --> 01:14:18,720
+بتحط حبه بتدي جزء من السكر اللي صح صحكفهدول الناس
+
+798
+01:14:18,720 --> 01:14:24,680
+طبعا بنعطيهم سكر حتى يتم يعني ارتفاع السكر
+
diff --git a/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/RwnvgFCBCRw_postprocess.srt b/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/RwnvgFCBCRw_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..847c2fd09e0fa55024ec3a3b9f0deb5ba8a8790b
--- /dev/null
+++ b/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/RwnvgFCBCRw_postprocess.srt
@@ -0,0 +1,3116 @@
+1
+00:00:20,790 --> 00:00:24,970
+بالنسبة لأسباب الحروق قلنا إنه الحرارة العالية
+
+2
+00:00:24,970 --> 00:00:30,790
+عشان يتم حرق للإنسان نحتاج إلى مصدر حرارة ونحتاج
+
+3
+00:00:30,790 --> 00:00:36,390
+إلى إيش؟ زمن أو مدة، مظبوط؟ وربنا سبحانه وتعالى
+
+4
+00:00:36,390 --> 00:00:41,050
+بيقول وإن منكم إلا واردوها كان على ربك حتما مقضية
+
+5
+00:00:41,050 --> 00:00:46,810
+هل كل الناس هينحرجوا في نهار جهنم؟ طب ما هو السبب؟
+
+6
+00:00:46,810 --> 00:00:52,940
+السبب أنهم يمرون عليها كالبرقبمعنى الزمن صفر
+
+7
+00:00:52,940 --> 00:00:59,240
+فبصيرش حروق ولا لأ فالحرارة العالية من الحاجات
+
+8
+00:00:59,240 --> 00:01:05,220
+اللى بتعمل قولنا النار الغاز السخن السوائل الساخنة
+
+9
+00:01:05,220 --> 00:01:09,540
+كمان المواد الكيمائية ممكن تعمل حروق مواد كيمائية
+
+10
+00:01:09,540 --> 00:01:14,660
+سواء قلويات أو أحمض حتى مشتقات البترول ممكن تعمل
+
+11
+00:01:14,660 --> 00:01:22,110
+حروق إذا مكثت مد على جسم الإنسانالأحماض و القلويات
+
+12
+00:01:22,110 --> 00:01:26,070
+ممكن تعمل حروق و الدار الحمد لله ملانة أحماض و
+
+13
+00:01:26,070 --> 00:01:32,090
+قلويات ميه تنار و الكلور المركز و الصودا الكاوية و
+
+14
+00:01:32,090 --> 00:01:35,710
+كل هذه الشغلات و مزيل الدهون كل هذه عبارة عن مواد
+
+15
+00:01:35,710 --> 00:01:40,810
+كيميائية الكهربا بتعمل حروق الإشعاعات حكينا عن
+
+16
+00:01:40,810 --> 00:01:46,060
+الإشعاعاتتعمل حروق في مخاطر إضافية للحروق يعني
+
+17
+00:01:46,060 --> 00:01:51,880
+الحرق لا يقتصر عند الناس اللي فيه مكانه أو لا
+
+18
+00:01:51,880 --> 00:01:55,780
+يقتصر عند الناس الموجودين في الحدث ممكن يأثر على
+
+19
+00:01:55,780 --> 00:02:02,140
+ناس تانين و ممكن يأثر على المكان بمعنى ممكن
+
+20
+00:02:02,140 --> 00:02:05,840
+الدخنة تعمل اختناق للناس اللي في المارة أو الناس
+
+21
+00:02:05,840 --> 00:02:12,060
+اللي في المبنى جنبنابمعنى انه فش غاز اكسوجين كفاية
+
+22
+00:02:12,060 --> 00:02:17,820
+يستنشق الإنسان فبيختنق فبتعمل ما يسمى اختناقات
+
+23
+00:02:17,820 --> 00:02:22,980
+الحاجة التانية انه الأبخرة المتساعدة من المواد
+
+24
+00:02:22,980 --> 00:02:25,960
+الكيميائية والحاجات الزادية بتمشي بحملها الجو و
+
+25
+00:02:25,960 --> 00:02:30,840
+بمشي و بتعمل برضه عشان هيك جاعدين يعني بيعملوا
+
+26
+00:02:30,840 --> 00:02:35,140
+لإيران و بيعملوا لكذا انهالاشعاعات النووية و مش
+
+27
+00:02:35,140 --> 00:02:38,880
+الاشعاعات النووية هذه ممكن تعمل مشاكل لأنها بتنحمل
+
+28
+00:02:38,880 --> 00:02:42,140
+بواسطة الريح و بتنتجل من المكان كمان الحرارة ممكن
+
+29
+00:02:42,140 --> 00:02:48,080
+تنحمل و الدخنة السخنة او الأبخار السخنة تنحمل من
+
+30
+00:02:48,080 --> 00:02:53,820
+مكان لمكان و تعمل اختناقات واحتراقات المبنى اللي
+
+31
+00:02:53,820 --> 00:03:00,370
+بنحرج بيصير قايل للسقوط ممكن أن ينهار و يدعالسجف
+
+32
+00:03:00,370 --> 00:03:07,890
+بطول عبارة عن اسمنت زي الحديد إذا تعرض للحرارة، شو
+
+33
+00:03:07,890 --> 00:03:12,770
+بيصير في الحديد؟ بتمدد فبتتكسر الاسمنت الاسمنت
+
+34
+00:03:12,770 --> 00:03:16,670
+اللي حواليه أو الصبّة اللي حواليه، شو بيصير فيها؟
+
+35
+00:03:16,670 --> 00:03:23,350
+بتتكسر، اتكسرت، صارت قايلة للسقوط فممكن يصير
+
+36
+00:03:23,350 --> 00:03:25,750
+انهيارات في المباني
+
+37
+00:03:28,560 --> 00:03:33,880
+الحروق إله درجات درجة الحروق إلها علاقة بالزمن
+
+38
+00:03:33,880 --> 00:03:39,280
+وشدة النار شدة النار و الزمن إذا كان النار شديدة
+
+39
+00:03:39,280 --> 00:03:43,480
+جدا بدون زمن ممكن مايصيرش حرق أو يصير حرق من درجة
+
+40
+00:03:43,480 --> 00:03:48,660
+الأولى إذا كان النار شديدة و زمن طويل جدا تعمل حرق
+
+41
+00:03:48,660 --> 00:03:53,280
+من الدرجة الثالثة أو الرابعة ماشي فالنار درجات
+
+42
+00:03:53,280 --> 00:04:01,080
+الحروق إلها عاملين أساسيااللي هي شدة الحرارة مع
+
+43
+00:04:01,080 --> 00:04:09,320
+مدة الحرق في أن قسم الحروق إلى عدة درجات الدرجة
+
+44
+00:04:09,320 --> 00:04:13,580
+الأولى وهي أبسط الدرجات طبعا اللي بتاخد الأولى
+
+45
+00:04:13,580 --> 00:04:17,860
+كطالبة بتكون جامعة أعلى العلامات لكن اللي بنحرك
+
+46
+00:04:17,860 --> 00:04:23,850
+درجة أولى ويكون أبسطالحروق يعني يعتبر بسيط جدا و
+
+47
+00:04:23,850 --> 00:04:28,950
+قد يعني يحتاج الى ولا شيء كيف بيصير هذا الحرج من
+
+48
+00:04:28,950 --> 00:04:33,610
+الدرجة الأولى مثلا؟ شو بيسببه؟
+
+49
+00:04:33,610 --> 00:04:38,910
+ايش بيسببه؟ التعرض لإشهاد الشمس واحد في هالصيف
+
+50
+00:04:38,910 --> 00:04:43,010
+الحلو الدنيا نار و يروح من الساعة تمانية تسعة
+
+51
+00:04:43,010 --> 00:04:48,110
+الصبح يظل على البحر لأذن المغرب اه؟ ايش بيصير فيه؟
+
+52
+00:04:48,950 --> 00:04:54,250
+بنحرج مش راضى يقعد فى الخيمة و بده يتصيف يعنى فى
+
+53
+00:04:54,250 --> 00:04:58,130
+واحد بيقولى إيش معناه فى المصطفين المصطفين يعني
+
+54
+00:04:58,130 --> 00:05:02,930
+اللى راحين وطشوا فى الصيف صح؟ و غالبا منهم بيروحوا
+
+55
+00:05:02,930 --> 00:05:07,650
+يا بيروحوا على البحر يا بيروحوا فى مناطق فاضية هيك
+
+56
+00:05:07,650 --> 00:05:14,890
+و يتصيف فبيكونوا عرضة لأشعة الشمس لفترة طويلة و
+
+57
+00:05:14,890 --> 00:05:19,340
+مصطفين يعني فى الصيفراحين يتنزعوا في الصيف الصيف
+
+58
+00:05:19,340 --> 00:05:27,080
+موصوب بإيه حرارة عالية ونمدة طويلة الدرجة الأولى
+
+59
+00:05:27,080 --> 00:05:33,280
+طبعا بيصير حمرار تغير لون الجل ورم خفيف بيصير
+
+60
+00:05:33,280 --> 00:05:43,370
+إحساس بالألم بسيط وغالبا بطيب بسرعة بدون ترك أيهذا
+
+61
+00:05:43,370 --> 00:05:52,050
+بالنسبة للدرجة الأولى ويقولنا بيسببه حرارة بسيطة
+
+62
+00:05:52,050 --> 00:05:58,250
+لمدة طويلة او حرارة اكتر شويه لمدة قصيرة جدا ممكن
+
+63
+00:05:58,250 --> 00:06:02,930
+تسبب الحرج من الدرجة الداخلية الحرج الدرجة التانية
+
+64
+00:06:05,700 --> 00:06:09,600
+نفس مصفات حرق الدرجة الأولى اللى انه بيصير في
+
+65
+00:06:09,600 --> 00:06:15,220
+الجسم فقاقيع بيصير في الجسم فقاقيع هذه الفقاقيع
+
+66
+00:06:15,220 --> 00:06:20,760
+تحتوي على البلازمة البلازمة الدم و اما بتلاقي
+
+67
+00:06:20,760 --> 00:06:24,160
+فجرية في جسم الإنسان بتقول هذا حرق من الدرجة
+
+68
+00:06:24,160 --> 00:06:31,440
+التانية طبعا بيكون عمق الحرق أكبر بيتأثر
+
+69
+00:06:31,440 --> 00:06:38,690
+الدرجات اللى أق تحت الجلدبصير احمرار و بصير نتوقات
+
+70
+00:06:38,690 --> 00:06:43,770
+و زى ما قلنا بصير فقاقية بكون ايه فى الفقاقية هاد
+
+71
+00:06:43,770 --> 00:06:48,690
+ملانة بطبجة من البلازمة الدم داخل الفقاعة بكون
+
+72
+00:06:48,690 --> 00:06:52,950
+طبجة من البلازمة الدم طبعا هاد بطبجة يعنى عبارة عن
+
+73
+00:06:52,950 --> 00:06:59,290
+شىء معقّم نظيف معقّم طاهر عشان هيك بنحرص أنه
+
+74
+00:06:59,290 --> 00:07:03,230
+مانجيمهاش الدرجة
+
+75
+00:07:03,230 --> 00:07:11,200
+الثالثةبتكون النار أشد الزمن أطول النار أشد الزمن
+
+76
+00:07:11,200 --> 00:07:22,960
+أطول زي مي سخنة مثلا كهربا نار أتباع النار سوائل
+
+77
+00:07:22,960 --> 00:07:29,000
+ساخنة كل هذه شغلات ممكن تعمل حروق طبعا بيصير حرق
+
+78
+00:07:29,000 --> 00:07:34,860
+للأنسجة العميقة كالجلب و بيصل للطبق القائديةو
+
+79
+00:07:34,860 --> 00:07:40,400
+العضلات و كذا ممكن كل هذه تتأثر بالبداية بيبدوا
+
+80
+00:07:40,400 --> 00:07:45,120
+لون الجلد أبيض ليش بيبدأ لون الجلد في الحرق من
+
+81
+00:07:45,120 --> 00:07:49,820
+درجة تالت أولها أبيض أو شاحب لأن الأوعية الدموية
+
+82
+00:07:49,820 --> 00:07:54,660
+بتنخبط و بصير فش فيها دم شو اللي معطي لونك أنت
+
+83
+00:07:54,660 --> 00:07:59,180
+اللون اللي انت فيه أن الأوعية الدموية موجودة مدينة
+
+84
+00:07:59,180 --> 00:08:03,900
+لونهابالاضافة طبعا لمادة قداش فيه ميلانين مافيش
+
+85
+00:08:03,900 --> 00:08:10,020
+ميلانين لكن البني آدم لما بيخاف بصفر وجهه يعني
+
+86
+00:08:10,020 --> 00:08:14,480
+بيصير أبيض بيصير لونه أبيض من ما يكون في الوضع
+
+87
+00:08:14,480 --> 00:08:20,540
+ليش؟ لأنه صار فش دم في الوجه مش فش بالمرة كمية
+
+88
+00:08:20,540 --> 00:08:25,040
+قليلة جدا من الدم في الوجه فبصفر بيصير إيش لونه
+
+89
+00:08:25,040 --> 00:08:32,780
+شاحب ففي حالة الحرق بيصير اللون شاحبأو أبيض الطبق
+
+90
+00:08:32,780 --> 00:08:36,960
+القاعدى للجلد بتتلف عشان هيك بيصيرش التقام او
+
+91
+00:08:36,960 --> 00:08:41,240
+يحتاج وقت طويل جدا للالتقام او بيصير التقام بوجود
+
+92
+00:08:41,240 --> 00:08:47,160
+تشوه او ندبات في جسم الإنسان وهذه الندبات يعني قد
+
+93
+00:08:47,160 --> 00:08:51,600
+تحتاج الى عمليات تجميل فيما بعد وزى ما قلنا في ناس
+
+94
+00:08:51,600 --> 00:08:57,970
+بيقولوا في درجة رابعة اللى هو عبارة عن حرقتفحين
+
+95
+00:08:57,970 --> 00:09:03,070
+حرق المنطقة لدرجة ان ده بيصير فحمة فبيطلع مين أكتر
+
+96
+00:09:03,070 --> 00:09:10,530
+حالات الحروق هذه حسب حالة المصاب بالظبط مين الأكتر
+
+97
+00:09:10,530 --> 00:09:15,470
+حسب حالة المصاب واحد تفحم أحد أصابع يده مثلا أو
+
+98
+00:09:15,470 --> 00:09:21,110
+أحد أصابع قدمه حتى لو قطع واتخلصنا منه بيظل الجسم
+
+99
+00:09:21,110 --> 00:09:26,870
+سليم وكويس غير عن أنه إنسان ينحرقأو يحرق بدرجة
+
+100
+00:09:26,870 --> 00:09:31,130
+مثلا ثالثة صدره و دهره ماخدين bad cocaine بصير
+
+101
+00:09:31,130 --> 00:09:36,610
+مشكلة وبناء عليه حالة الجسم أو حالة المصاب هي التي
+
+102
+00:09:36,610 --> 00:09:43,410
+تقدر اللي هو الإصابة حكينا عن مساعة السطح ذاكرين؟
+
+103
+00:09:43,410 --> 00:09:49,330
+ذاكرين مساعة السطح بتهمنا
+
+104
+00:09:49,330 --> 00:09:55,530
+في إيش؟في كيفية التعامل مع المقصاد واحد عنده حرق
+
+105
+00:09:55,530 --> 00:10:04,630
+نسبة في المية كيف نتصرف معاه احنا بكل بساطة في
+
+106
+00:10:04,630 --> 00:10:10,310
+قاعدة عشرة في المية في الكبار البالغين السليمين
+
+107
+00:10:10,310 --> 00:10:17,510
+ليس في حاجة الى دخول المستشفى غالبا بنجوسين ايش؟
+
+108
+00:10:17,510 --> 00:10:22,630
+غالبا ليش غالبا؟ لأن في حالاتأجل من هيك تحتاج إلى
+
+109
+00:10:22,630 --> 00:10:27,430
+دخول مستشفى التلاتين في المية لازم يدخل مستشفى
+
+110
+00:10:27,430 --> 00:10:35,650
+أكتر من خمسين في المية يشكل خطر على حياة الإنسان
+
+111
+00:10:35,650 --> 00:10:41,650
+شكل خطر على حياة الإنسان احنا جولنا غالبا ايش يعني
+
+112
+00:10:41,650 --> 00:10:46,870
+كده جولنا غالبا يعني في استثنائة ماجولناش على
+
+113
+00:10:46,870 --> 00:10:50,240
+إطلاقاذا كان على اطلاق يبقى هذا القاعدة بتمشي على
+
+114
+00:10:50,240 --> 00:10:55,340
+الجميع لكن غالبا يبقى في استثناء ايش الاستثناء من
+
+115
+00:10:55,340 --> 00:10:59,620
+الموضوع الاستثناء
+
+116
+00:10:59,620 --> 00:11:07,280
+العمر الأطفال عشرة فالمية عندهم مش قليل الطفل عشرة
+
+117
+00:11:07,280 --> 00:11:11,500
+فالمية من مساحة جسمه بده مستشفى الشايخوخة
+
+118
+00:11:12,530 --> 00:11:17,830
+الاختيارية 10% برضه من مساحة جسمه تحتاج دخول
+
+119
+00:11:17,830 --> 00:11:27,030
+مستشفى كمان نعم كمان من الحاجات اللي .. المكان او
+
+120
+00:11:27,030 --> 00:11:32,530
+صحة البني ادم واحد مريض سكر واحد مريض ضغط واحد
+
+121
+00:11:32,530 --> 00:11:37,310
+مريض قلب واحد مريض او اي دموية واحد مريض ممكن
+
+122
+00:11:37,310 --> 00:11:42,390
+يحتاج دخول مستشفى بنسبة حرق قليلةزي ما قالت أختنا
+
+123
+00:11:42,390 --> 00:11:51,650
+المكان الإصابة في الأطراف أصابع الإيديان أو أصابع
+
+124
+00:11:51,650 --> 00:11:56,810
+الرجلين كدهش بين أصابع إيديكي و العظمة كدهش بينه
+
+125
+00:11:56,810 --> 00:12:01,110
+مافيش لحمة مافيش حاجة بسيطة جدا فبكل بساطة هذه
+
+126
+00:12:01,110 --> 00:12:06,150
+ممكن مع الحرج تروح إيش حيصير لو راحت حتى تساقط
+
+127
+00:12:06,150 --> 00:12:11,370
+الأصابع أنا بعرف ناس انحرجوا وأصابعهم موجةفهذا
+
+128
+00:12:11,370 --> 00:12:15,010
+لازم يكون تحت رعاية طبية عشان ما يخششه مضاعفات
+
+129
+00:12:15,010 --> 00:12:19,430
+ماشي؟ فالأطراف سواء أطراف الإيدين أو أطراف الرجلين
+
+130
+00:12:19,430 --> 00:12:24,850
+يجب أنه يهتموا فيهم و يدخلوا مستشفى كمان منطقة
+
+131
+00:12:24,850 --> 00:12:32,490
+الفم والأنف والرجبة هذه المنطقة إيش دورها؟ التنفس
+
+132
+00:12:32,490 --> 00:12:39,070
+كمان أخطر من الأكل ولا لأ؟ التنفس مهم جدا بعد قليل
+
+133
+00:12:39,070 --> 00:12:43,960
+بعد قليلستتورم هذه المنطقة إذا تورمت هذه المنطقة
+
+134
+00:12:43,960 --> 00:12:51,900
+ماذا ينتج؟ سكر مجرى النفس إذا أنت بيصير عندك ذوكان
+
+135
+00:12:51,900 --> 00:13:00,460
+أو رشح تقدر تتنفسي من أنفك بسكر خالص إيش صار جوا؟
+
+136
+00:13:00,460 --> 00:13:03,840
+ورمت المنطقة بالظبط اللي بيصير في الرشح أو في
+
+137
+00:13:03,840 --> 00:13:11,260
+الذوكان أو في تسكير الأنف أنه المنطقة جوا ترمورمت
+
+138
+00:13:11,260 --> 00:13:16,120
+سكرت النفس فالحرق بيعمل ورم من أبسط يعني أمور
+
+139
+00:13:16,120 --> 00:13:19,860
+الحرق أنه بعد الحرق بيصير ورم فلو صار ورم في الفم
+
+140
+00:13:19,860 --> 00:13:25,980
+و الأنف سكر مجرى النفس ماشي فالناس اللي في منطقة
+
+141
+00:13:25,980 --> 00:13:31,200
+التنفس يحتاجوا إلى دخول مستشفى اللي هو حروق الرقبة
+
+142
+00:13:31,200 --> 00:13:36,210
+و الوجاهماشي تحتاج الى دخول مستشفى ومنطقة دي
+
+143
+00:13:36,210 --> 00:13:41,390
+بقولنا الكفين و القدمين
+
+144
+00:13:41,390 --> 00:13:49,610
+تحتاج الى دخول مستشفى ماشي منطقة العانة منطقة مهم
+
+145
+00:13:49,610 --> 00:13:54,450
+جدا كل منطقة العانة كلها واحد فالمية لكن الحرق في
+
+146
+00:13:54,450 --> 00:13:58,910
+هذه المنطقة يحتاج دخول مستشفى ليه؟ بده يفور كمان
+
+147
+00:13:58,910 --> 00:14:03,140
+شوية الطفل او الكبير او الصغير او كدهإيش أنا بده
+
+148
+00:14:03,140 --> 00:14:09,620
+بول؟ يعني إذا كانش المكان مفتوح هيتفجر هتنفجر
+
+149
+00:14:09,620 --> 00:14:14,320
+المثانة و هيسير يخش في مشاكل فبكل بساطة أول ما
+
+150
+00:14:14,320 --> 00:14:19,680
+يدخل المستشفى أما بدخلوله جسطرة و أما بيفتحوله ..
+
+151
+00:14:19,680 --> 00:14:24,880
+بدخلوله من تحت الصرة فوق المثانة فبصير البول يطلع
+
+152
+00:14:24,880 --> 00:14:30,150
+منه يبقى اتريحنا من حكاية أنه تنفجرالمثالة هذه
+
+153
+00:14:30,150 --> 00:14:34,230
+مناطق حتى لو كانت الناس بقدر ما تكون تحتاج إلى
+
+154
+00:14:34,230 --> 00:14:40,870
+دخول مستشفى حكينا إنه سطح الجسم جدير 16 في ال ..
+
+155
+00:14:40,870 --> 00:14:48,930
+16 متر واحد وست من عشرة واحد وست متر مربع ماشي
+
+156
+00:14:48,930 --> 00:14:53,290
+طبعا هذا في المتوسط في ناس ممكن يكونوا أقل كتير
+
+157
+00:14:53,290 --> 00:14:57,790
+وفي ناس ممكن يكونوا أكتر كتيريعني بتشوفي واحدة
+
+158
+00:14:57,790 --> 00:15:01,770
+صلاة النبر بتخشش منها الباب مش هيطلع .. مش هتطلع
+
+159
+00:15:01,770 --> 00:15:06,130
+واحد وست من عشرة و بتشوفي واحدة يعني أضعف منها ال
+
+160
+00:15:06,130 --> 00:15:11,530
+computer مش هتطلع واحد وست من عشرة، مظبوط؟ وبناء
+
+161
+00:15:11,530 --> 00:15:16,410
+عليه المساحة بالمتوسط تعتبر واحد وست من عشرة
+
+162
+00:15:16,410 --> 00:15:22,790
+ذاكرين كيف جسمناها؟ جسمناها بنظام اسم نظام إيش؟
+
+163
+00:15:22,790 --> 00:15:24,370
+نظام التسعة، كيف قلناها؟
+
+164
+00:15:29,260 --> 00:15:35,860
+الساق احنا قلنا عملنا ال .. الاساس اليد الذراع
+
+165
+00:15:35,860 --> 00:15:41,040
+الذراع ماشي قلنا بناء على الذراع بتقدري تجيبي كل
+
+166
+00:15:41,040 --> 00:15:45,480
+الباقية الذراع قداش تسعة احنا عندنا اكتر ذراع
+
+167
+00:15:45,480 --> 00:15:53,000
+هيصار تسعتين الذراع قد الإجر او قد الساق قد الساق
+
+168
+00:15:53,000 --> 00:15:58,890
+قداش تسعتين في الكتاب المرسوم عندكم ايشمين تعرف
+
+169
+00:15:58,890 --> 00:16:02,490
+تجراها من الكتاب؟
+
+170
+00:16:02,490 --> 00:16:10,210
+لا .. لا .. بقعد .. نعم؟ إيش شايفين ع الدراق؟ فيه
+
+171
+00:16:10,210 --> 00:16:14,650
+مرسوم اثنين .. واحد من الوجه و واحد من الجفا مش
+
+172
+00:16:14,650 --> 00:16:19,850
+هيك؟ و مكتوب ع دراقة واحدة فقط تسعة، مظبوط؟ طب
+
+173
+00:16:19,850 --> 00:16:26,090
+انزلوا تحت ع الساق مكتوب عدل أربعة .. مكتوب عدل
+
+174
+00:16:26,090 --> 00:16:32,890
+أربعة، إيش بتفهم منها؟ان كل قدم تسعتين يعني هذا من
+
+175
+00:16:32,890 --> 00:16:38,990
+الأمام تسعة جسمها و من الخلف تسعة لكن الذراع من
+
+176
+00:16:38,990 --> 00:16:44,570
+الأمام و الخلف يعتبر تسعة واحدة فصار عندي الساق
+
+177
+00:16:44,570 --> 00:16:52,190
+تسعتين عندي ساقين يبقى اربع تسعات و ذراعين ست
+
+178
+00:16:52,190 --> 00:16:59,300
+تسعات مش هيكقلنا الذراع جد غطاء الكم او ال .. هذا
+
+179
+00:16:59,300 --> 00:17:04,220
+بيجي .. بيغطي البطن والصدر يبقى الصدر والبطن يسوى
+
+180
+00:17:04,220 --> 00:17:09,620
+تسعة اتين والصدر والبطن تماما جد و الظهر، مظبوط؟
+
+181
+00:17:09,620 --> 00:17:13,480
+كمان تسعة اتين، هاي اربع تسعات، صار عندي جد ايش؟
+
+182
+00:17:13,480 --> 00:17:17,860
+عشر تسعة، صح؟ ضال تسعة، حكينا عن الوجه و الراس و
+
+183
+00:17:17,860 --> 00:17:23,370
+ال .. و الرجبةماجيبناش سئلة هون هي أخدم تسعة صار
+
+184
+00:17:23,370 --> 00:17:27,790
+احداشر تسعة في تسعة احداشر في تسعة وظال واحد في
+
+185
+00:17:27,790 --> 00:17:33,590
+المية اللي هي منطقة العالم واضح هذا بالنسبة لكيف
+
+186
+00:17:33,590 --> 00:17:40,790
+جسمنا التسعة في حد عنكم استعدادة عدوها نرجع
+
+187
+00:17:40,790 --> 00:17:45,890
+تاني مين تقولنا كيف جسمنا الجسم
+
+188
+00:17:56,500 --> 00:18:03,680
+تفضل الذراع كل ذراع تسعة أربع
+
+189
+00:18:03,680 --> 00:18:11,460
+تسعات الساقين اتنين و اتنين البطن والصدر لأ البطن
+
+190
+00:18:11,460 --> 00:18:18,860
+والصدر تسعة تاني تمانتاش مع بعض و الظهر تسعة تاني
+
+191
+00:18:18,860 --> 00:18:22,680
+تسعة
+
+192
+00:18:23,720 --> 00:18:32,760
+بظل ايش واحد لمنطقة العالم ماشي ايش
+
+193
+00:18:32,760 --> 00:18:37,260
+بفيدنا قولنا هذا الكلام بيعلمنا كيفية التعامل مع
+
+194
+00:18:37,260 --> 00:18:46,860
+الحالات قولنا لعند عشرة في المية غالبا لا يحتاج
+
+195
+00:18:46,860 --> 00:18:54,420
+دخول مستشفى غالبا مش على اطلاق30% فما فوق بده
+
+196
+00:18:54,420 --> 00:19:03,140
+مستشفى تحتاج دخول مستشفى 50% فما فوق خطر على حياة
+
+197
+00:19:03,140 --> 00:19:11,480
+الإنسان ماشي لكن المناطق المستثناء من القاعدة هذه
+
+198
+00:19:11,480 --> 00:19:17,830
+اللي هي منطقة الأطرافالإيدين والرجلين الأصابع
+
+199
+00:19:17,830 --> 00:19:22,330
+تحتاج دخول مستشفى جد ما كانت النسب القليلة منطقة
+
+200
+00:19:22,330 --> 00:19:26,590
+الأنف والفم والرجبة تحتاج دخول مستشفى جد ما تكون
+
+201
+00:19:26,590 --> 00:19:33,170
+مسابق جريبة منطقة العانجلنه تحتاج دخول مستشفى كيف
+
+202
+00:19:33,170 --> 00:19:42,310
+احنا بنسعف حالات الحروق في .. في القديم كان يعني
+
+203
+00:19:42,310 --> 00:19:46,340
+اللي بديه يعمل حاله شاطرالواحد اللي هو حاطط على
+
+204
+00:19:46,340 --> 00:19:50,520
+حاله مادة مشتعلة و مولّع في حاله النار زي ال ..
+
+205
+00:19:50,520 --> 00:19:55,360
+البو عزيزي هذا بتاع تونس و .. ومشت عند كتير من
+
+206
+00:19:55,360 --> 00:19:58,800
+الناس يعني صاروا يحسبوها شطارة فحين انه هذا انتحار
+
+207
+00:19:58,800 --> 00:20:03,080
+لا يجوز شرعا و اللي بحرج نفسه بيظل ربنا .. اللي
+
+208
+00:20:03,080 --> 00:20:08,660
+بقتل نفسه بيظل ربنا في النار يعذبه بالشيء اللي عذب
+
+209
+00:20:08,660 --> 00:20:13,060
+نفسه فيه بالاضافة إلى نار جهنم مش بده فهي الغالب
+
+210
+00:20:13,060 --> 00:20:19,210
+اللي واحداحنا قادرين على ولا شي فبنقن عليه يعني مش
+
+211
+00:20:19,210 --> 00:20:26,170
+شطار أن الواحد يحرج نفسه ف زمان كان الواحد يعني زي
+
+212
+00:20:26,170 --> 00:20:30,770
+ما بيقولوا إذا يعني خدشتي حاسيسه ولا شعرت .. جرحت
+
+213
+00:20:30,770 --> 00:20:35,230
+مشاعره ولا أبصرتشه يعني أي كلمة بسيطة يروح اللي هو
+
+214
+00:20:35,230 --> 00:20:40,570
+جال يعني بده يتخلص من الدنيا و .. بده ينتحر فيدير
+
+215
+00:20:40,570 --> 00:20:46,990
+حاله بدزيم كذا و يولف حاله معاههذا الكلام ايش كان
+
+216
+00:20:46,990 --> 00:20:50,690
+بيصير مع الحالات مجرد ما تصيب النار جسمه مين
+
+217
+00:20:50,690 --> 00:20:55,370
+بيلاقيه في وجهه يدب حاله يعني ان حين صار بده حياة
+
+218
+00:20:55,370 --> 00:20:59,130
+طب احنا بنترجح اننا يومين تلات اسبوع بيصيرش و
+
+219
+00:20:59,130 --> 00:21:04,810
+اتحمل و .. ابدا مجرد ما صابت النار جسمه صار ايش؟
+
+220
+00:21:04,810 --> 00:21:10,240
+بيجري على الحياة طبعا ايش بيصير؟ بتشوهبقعد أشهر في
+
+221
+00:21:10,240 --> 00:21:14,760
+المستشفيات وجهه بيصير عشجة، إيده بتبطل تفتح، شفتوا
+
+222
+00:21:14,760 --> 00:21:20,280
+حالات المحرجين كتير كيف؟ وضعهم بيصير كرب وكل يوم و
+
+223
+00:21:20,280 --> 00:21:24,000
+اتانى هو داخل عامل عملية تجميل و صار خش في
+
+224
+00:21:24,000 --> 00:21:28,560
+التهابات و خش في اكتئاب نفسه و خش و خش و سعر هو
+
+225
+00:21:28,560 --> 00:21:32,680
+نفسه يستحمل حاجةفمكان ربنا اللي قد خلقنا الإنسان
+
+226
+00:21:32,680 --> 00:21:37,660
+فيه أحسن تقول إن كان شكلك محترم و مجبول و تمام
+
+227
+00:21:37,660 --> 00:21:42,280
+تمام دير بالك على حالة بدهوش فالحين صار بدهو دير
+
+228
+00:21:42,280 --> 00:21:48,340
+باله على حاله بعد ما صار في وضع مش طبيعي فاحنا
+
+229
+00:21:48,340 --> 00:21:52,980
+عشان هي كده مجرد ما ينحرج الواحد بدب حاله على أقرب
+
+230
+00:21:52,980 --> 00:21:58,390
+بني آدمممكن يحرج البني أدم اللى بده إياه يسعفه و
+
+231
+00:21:58,390 --> 00:22:04,050
+أنا شوفت صدقه حالات كتيرة مسعفين إيديهم محرجات جاي
+
+232
+00:22:04,050 --> 00:22:09,430
+طف واحد فمحرج إيديه عشان إحنا يعني نطف الإنسان
+
+233
+00:22:09,430 --> 00:22:15,010
+لازم يعني ماتخليهوش ييجي فينا كيف ممكن أعمل؟ كل
+
+234
+00:22:15,010 --> 00:22:19,470
+بساطة جيب بطنيه ولفه عندك بطنيه لفه مافيش بطنيه
+
+235
+00:22:19,470 --> 00:22:24,810
+خليه يتدحرج على الأرض واقع دهره محروجلو نام ع دهرو
+
+236
+00:22:24,810 --> 00:22:30,930
+ايش بيصير فيه؟ ليش بنطفي طيب؟ مافيش أكسجين بالظبط
+
+237
+00:22:30,930 --> 00:22:35,910
+لو واحد مهروب من السدر ونام على ال .. على صدره بت
+
+238
+00:22:35,910 --> 00:22:39,930
+.. بتنطفي النوم فبنخلي يده حرجة على الأرض ناخدين
+
+239
+00:22:39,930 --> 00:22:44,970
+بالكوه أو بنلفه بحرام بحيث أنه نمنع الأكسجين يخش
+
+240
+00:22:44,970 --> 00:22:50,030
+على منطقة الحراج و بنطفيه الحاجة اللي بعدها
+
+241
+00:22:52,950 --> 00:22:57,530
+بدنا نوفر ما احنا قلناه ان كل بينا ولا واحد عارف
+
+242
+00:22:57,530 --> 00:23:04,510
+السؤال و لا لأ سألنا كذا سؤال ولا واحد بجاول فقلنا
+
+243
+00:23:04,510 --> 00:23:07,990
+بنرجعوا ع السريع احنا بنحكي هذا الكلام كان بده
+
+244
+00:23:07,990 --> 00:23:11,230
+اربع ساعات او ساعتين و شوية المهم ماشينا ع السريع
+
+245
+00:23:11,230 --> 00:23:16,290
+و بعدين بننقلوا على اقرب مركز طبي اسعاف الحروج من
+
+246
+00:23:16,290 --> 00:23:18,610
+الدرجة الاولى مافيش خطورة كتير من الحروج من الدرجة
+
+247
+00:23:18,610 --> 00:23:24,550
+الاولى فمجرد يعني ميه باردةممكن لدرجة التانية
+
+248
+00:23:24,550 --> 00:23:29,290
+ممنوع نجيب الفقاعات بالاضافة للماء البارد و بنحطش
+
+249
+00:23:29,290 --> 00:23:34,710
+ماء مثلج الفقاعات بنحطش ماء مثلج لإنه هتعمل ثلج في
+
+250
+00:23:34,710 --> 00:23:38,850
+الفقاعة و تخشش الواحد في المشاكل حرق الدرجة
+
+251
+00:23:38,850 --> 00:23:42,910
+التالتة مافيش ملابس تنجام لإن الملابس ملزقة و
+
+252
+00:23:42,910 --> 00:23:48,770
+مسكرة السوائل اللي بتطلع من الجسم بنحط طبقة انغطية
+
+253
+00:23:48,770 --> 00:23:53,790
+يعني بحرام شاشة اشي زي هيالاطراف طبعا نرفعها إلى
+
+254
+00:23:53,790 --> 00:24:00,110
+أعلى عشان ما ترمش و بنمنعه أنه يتحرك لأن الحركة
+
+255
+00:24:00,110 --> 00:24:05,870
+بيصير بدها دم و سوائل و إش زي هيك و بنتبه للوجه و
+
+256
+00:24:05,870 --> 00:24:10,690
+للتنفس إذا كان في إصابة في الوجه بنحطش ماء مطلقا
+
+257
+00:24:10,690 --> 00:24:15,090
+على حرج من الدرج التالتة واحد محروب من الدرج
+
+258
+00:24:15,090 --> 00:24:17,850
+التالتة رغم أنه أكبر من الدرج الأولى و درج تانية
+
+259
+00:24:17,850 --> 00:24:25,380
+بيحطش عليه ماء مين عارف ليش بنحطش ماءبالظبط المي
+
+260
+00:24:25,380 --> 00:24:29,760
+هذه هتمص السوائل و الأملح اللى في جسمه هتسوي صدمة
+
+261
+00:24:29,760 --> 00:24:35,500
+ازيادة و تسوي مشكلة اكبر اذا بدنا نعطيه ميه بنسجيه
+
+262
+00:24:35,500 --> 00:24:43,280
+ميه سجية بنسجيله ميه نسبة واحد الى نص صودا وملح
+
+263
+00:24:43,280 --> 00:24:49,680
+كربونا مع ملح على لتر ميه و بنسجيه و ممنوع ياخد
+
+264
+00:24:49,680 --> 00:24:55,790
+مادة كحولى لان الكحول فى ديننا حرامفي حروق المواد
+
+265
+00:24:55,790 --> 00:25:01,690
+الكيميائية هنا وصلنا حروق
+
+266
+00:25:01,690 --> 00:25:04,930
+المواد الكيميائية برضه بدنا نزيل المادة الحارقة و
+
+267
+00:25:04,930 --> 00:25:09,950
+بدنا نغسل أثار أثار المادة الحارقة جيدا و نعدلها و
+
+268
+00:25:09,950 --> 00:25:15,930
+حكينا عن قصة الفلفل ذاكره ايش بتفهم ان الفلفل بضل
+
+269
+00:25:15,930 --> 00:25:20,570
+يحرج انه بضل الجسم محتفظ في مساماته في هذه المادة
+
+270
+00:25:20,570 --> 00:25:26,450
+رغم اني غسلت لما ازهجتفبدي أعادل المادة المتبقية
+
+271
+00:25:26,450 --> 00:25:31,810
+على الجسم بالقلوي
+
+272
+00:25:31,810 --> 00:25:35,490
+بحامض و الحامض بقلوي بس حامض و قلوي من اللي بدرش
+
+273
+00:25:35,490 --> 00:25:40,390
+الجسم يكون حامض زي الليمون مثلا و قلوي زي الكربونة
+
+274
+00:25:40,390 --> 00:25:45,910
+حروق العين يوضع المصاب على الجانب السليم الجانب
+
+275
+00:25:45,910 --> 00:25:50,850
+المصاب الجانب المصاب و بندير ماءبحيث أنه مايجيش
+
+276
+00:25:50,850 --> 00:25:56,350
+الميه على العين السليمة و بننظفه و بنوديه على أقرب
+
+277
+00:25:56,350 --> 00:26:02,150
+مركز طبي بعدين عضت التلج، عشان وصلنا؟ عضت الثلج
+
+278
+00:26:02,150 --> 00:26:07,270
+أثرية الثلج له أسنان و بقاط صح ولا لأ؟ إيش عن عض
+
+279
+00:26:07,270 --> 00:26:11,330
+التلج؟
+
+280
+00:26:11,330 --> 00:26:17,330
+اتعرض الإنسان لدرجة حرارة منخفضة جدا بتأثر على كل
+
+281
+00:26:17,330 --> 00:26:21,790
+جسمتأثر على كل الجسم فتعمل ما يسمى بعضة التلج
+
+282
+00:26:21,790 --> 00:26:26,370
+وتحدث عندما تعرض للراحة الحرارة وتزداد خطورتها
+
+283
+00:26:26,370 --> 00:26:31,550
+بالزمن زي الحرج كل ما زاد الزمن كل ما زاد ايه
+
+284
+00:26:31,550 --> 00:26:35,410
+الأثار فوين
+
+285
+00:26:35,410 --> 00:26:41,570
+في مناطق بنلاقي فيها حرارة متخفضة جبل الشيخ ليش
+
+286
+00:26:41,570 --> 00:26:48,050
+اسمه جبل الشيخ؟ من عارف؟ لأنه لابس طيب طول السنة
+
+287
+00:26:48,840 --> 00:26:56,020
+فجيه بيضة اللي هي التلج فسموه جبل الشيخ فيه برد في
+
+288
+00:26:56,020 --> 00:26:59,240
+المنطقة و بعدين معروف يعني كل مرتفع الإنسان عن سطح
+
+289
+00:26:59,240 --> 00:27:06,660
+البحر 150 متر بتخف درجة الحرارة أظن درجة كل 150
+
+290
+00:27:06,660 --> 00:27:13,200
+متر طب لما جبل يكون يعني حوالي 3000 متر عن سطح
+
+291
+00:27:13,200 --> 00:27:18,660
+البحر كثير بيخس حرارة فبصير تلج وبناء عليهاالمناطق
+
+292
+00:27:18,660 --> 00:27:23,660
+المرتفعة .. المناطق اللي فيها رطوبة و ريح .. فيها
+
+293
+00:27:23,660 --> 00:27:29,280
+رطوبة و ريح ممكن جدا الواحد يسير يرجف من كتر البرد
+
+294
+00:27:29,280 --> 00:27:34,840
+اتعالى كثير من الناس بيشكي انه انا عندي صداع و
+
+295
+00:27:34,840 --> 00:27:39,480
+مناخير مسددة و دايما مرشح بسأل لو انت ساكن علي اي
+
+296
+00:27:39,480 --> 00:27:42,540
+دور كان جالك على الدور الرابع او الخامس قلكوا شباك
+
+297
+00:27:42,540 --> 00:27:45,040
+غربي على الهواء جال اقعد .. تقعد على الشباك جال
+
+298
+00:27:45,040 --> 00:27:49,460
+اقعد نهيت الدنيا شغلفببقى جاي الهواء بارد جدا
+
+299
+00:27:49,460 --> 00:27:58,520
+بيعمله دفعات برد فالمنطقة المرتفعة و بالهواء
+
+300
+00:27:58,520 --> 00:28:03,120
+السريع بيعمل .. او الهواء الرطوبة كمان كل هذه
+
+301
+00:28:03,120 --> 00:28:06,400
+شغلات انت عادوا في السعودية مثلا الحرارة تصل فوق
+
+302
+00:28:06,400 --> 00:28:13,160
+الأربعين و الناس بيحسوش ليش بيحسوش؟ مافيش رطوبة
+
+303
+00:28:13,160 --> 00:28:17,390
+مافيش رطوبة لكن احنا على جنب البحرالرطوبة عالية
+
+304
+00:28:17,390 --> 00:28:22,870
+جدا، الرطوبة عالية جدا، في المناطق اللي فيها رطوبة
+
+305
+00:28:22,870 --> 00:28:30,390
+وفيها بخار ميه وإيش زي هيك، بتسبب اللي هي انخفاض
+
+306
+00:28:30,390 --> 00:28:36,750
+في درجات الحرارة، التلاجات، اللي يوم في التلاجة
+
+307
+00:28:36,750 --> 00:28:41,680
+بتخش فيها مكتورةمظبوط تلوجة بتخش فيها وقت مجطورة
+
+308
+00:28:41,680 --> 00:28:46,920
+كيب بتاعي نفسه اللحمة المثلجة و الجاج المثلج و
+
+309
+00:28:46,920 --> 00:28:52,420
+السمك المثلج كيب بيساوي هدول بيجيبوا مقاطير و
+
+310
+00:28:52,420 --> 00:28:55,340
+بيدخلوها و بتسيلوا نازلوا و عنها دي إيه اللي هي
+
+311
+00:28:55,340 --> 00:29:00,080
+يعني مواصفاتها الخاصة ففي بعض الناس بتشتغل في وسط
+
+312
+00:29:00,080 --> 00:29:04,600
+التلاجة و عشان تشتغل التلاجة بالطريقة الصحية لازم
+
+313
+00:29:04,600 --> 00:29:07,760
+يكون حرارتها متخفضة جدا بتهيألى خمسة و عشرين تحت
+
+314
+00:29:07,760 --> 00:29:12,270
+الصفريعني تلاجاتنا في الدور بيظبطنش لهيك شغل تخزن
+
+315
+00:29:12,270 --> 00:29:16,650
+فيها بعد أسبوع زمان بتحسيه يتغير لكن بتجيبيه من
+
+316
+00:29:16,650 --> 00:29:22,410
+عند الراجل كأنه جديد ليش؟ درجة حرارته متخفضة جدا
+
+317
+00:29:22,410 --> 00:29:28,130
+وبناء عليها هذه المناطق ممكن تسبب ما يسمى عضة
+
+318
+00:29:28,130 --> 00:29:36,710
+البرد في في الجسم مناطق مهم تتدفى ومهم ما تتعرضش
+
+319
+00:29:36,710 --> 00:29:41,740
+لعضة البرد اللي هي مناطق إيش؟الاطراف، الاطراف
+
+320
+00:29:41,740 --> 00:29:47,320
+منطقة بسيطة وقوايات الدموي جليلة، جلبها جليل،
+
+321
+00:29:47,320 --> 00:29:52,560
+عضلاتها جليلة، فبكل بساطة بتتأثر بالبرد، اطراف
+
+322
+00:29:52,560 --> 00:29:56,660
+الإيدين واطراف الإيزين، كمان منطقة الفم والأنف
+
+323
+00:29:56,660 --> 00:30:04,430
+والوجه، أخدين بالكواكير؟تعمل يعني سهولة انه يتأثر
+
+324
+00:30:04,430 --> 00:30:09,370
+الإنسان في البرد فهذه المناطق يعني لازم طلعتوا
+
+325
+00:30:09,370 --> 00:30:14,990
+انتروس مثلا يبقوا لابسين كمامات و لابسين في إيديهم
+
+326
+00:30:14,990 --> 00:30:19,310
+و الدنيا عندهم حرارة دايما دايما بردة و مع ذلك
+
+327
+00:30:19,310 --> 00:30:23,070
+بشتغلوا بروحوا بيجوا تمام تمام فهذه المناطق أكثر
+
+328
+00:30:23,070 --> 00:30:28,770
+مناطق أرضة لعضة التلج في الإنسان و كمان ذنين ممكن
+
+329
+00:30:28,770 --> 00:30:35,590
+يتأثرواإيش الأعراض اللي بيصير؟ الأعراض اللي بيصير
+
+330
+00:30:35,590 --> 00:30:42,950
+سببها أنه سوائل في جسم الإنسان بتبرد لما تبرد
+
+331
+00:30:42,950 --> 00:30:47,750
+بيصير تلج تصل لو درجة .. لما بتصير تلج بيصير مش دم
+
+332
+00:30:47,750 --> 00:30:54,530
+بيصير لها لون باهد اللون بيصير باهد الإنسان في
+
+333
+00:30:54,530 --> 00:31:00,370
+الأول بيحس بألم بسيط أو قد لا يشعر بألم اليوم يا
+
+334
+00:31:00,370 --> 00:31:06,850
+صبايابيحرجوا بالثلج معاكوا الخبر؟ وبيبنجوا بالثلج
+
+335
+00:31:06,850 --> 00:31:11,050
+يعني بيدهم يشتغلوا يقفوا يشتغلوا في مكان معين ممكن
+
+336
+00:31:11,050 --> 00:31:16,730
+يثلجك هذا العضو ويفتح فيه ويشتغل لأنه بيخف الاحساس
+
+337
+00:31:16,730 --> 00:31:20,450
+مع الثلج فالناس اللي بيصير عنده قطب الثلج ممكن في
+
+338
+00:31:20,450 --> 00:31:24,170
+الأول يبدأ يحس ألم بسيط وبعد هيك مايحسش خالص
+
+339
+00:31:24,170 --> 00:31:30,570
+مايحسش خالص لأنه الجهاز العصبي اتأثر من البرودة
+
+340
+00:31:30,570 --> 00:31:36,800
+العاليةبعد هيك إن ضلوا الواحد يعني في منطقة باردة
+
+341
+00:31:36,800 --> 00:31:45,880
+ممكن جدا يصير تأثير جسمي كامل بمعنى أنه مخه بتأثر،
+
+342
+00:31:45,880 --> 00:31:50,420
+جهاز الدولة الدموية بتأثر، القلب بتأثر، الرأتين
+
+343
+00:31:50,420 --> 00:31:57,860
+بتأثر، بموت يعني بتفلطح، بتكبر و بيصير عنده صدمة
+
+344
+00:31:57,860 --> 00:32:03,340
+عصبية مابقدرش يعني يقومها و بموتانا بعرف طبعا أيام
+
+345
+00:32:03,340 --> 00:32:07,840
+زمان و احنا صغار كانوا بخرفونا ان في طبعا غالبا
+
+346
+00:32:07,840 --> 00:32:14,300
+الفجرة شاطرين غالبا الفجرة شاطرين ليش؟ لأن هاي
+
+347
+00:32:14,300 --> 00:32:17,420
+البيارة بتاعتهم و هي مصدر الرزق يعني و هي الشغل و
+
+348
+00:32:17,420 --> 00:32:23,580
+العمل ولا لأ لكن الأبوه عنده مثلا شركات ولا عنده
+
+349
+00:32:23,580 --> 00:32:29,090
+مؤسسات ولا عنده مصانع ولا عنده ولا عندهما هو يا
+
+350
+00:32:29,090 --> 00:32:31,710
+ابني احنا نسيب المدرسة تعادى اشتغل احنا بدنا حد
+
+351
+00:32:31,710 --> 00:32:38,170
+يشتغل يمسك مالنا مش هيك فكان في ولد شاطر و فجير
+
+352
+00:32:38,170 --> 00:32:42,130
+وولد مبسوط و مكيف يعنى الولد الشاطر و الفجير مش
+
+353
+00:32:42,130 --> 00:32:46,950
+عندهم كهربا وولا عندهم دفات ولا عندهم اشي و الفجير
+
+354
+00:32:46,950 --> 00:32:49,670
+.. الولد الفرج ساكنين على التاني و اللي هو على
+
+355
+00:32:49,670 --> 00:32:53,650
+التالت فكان الولد الفجير هذا الشاطر يروح تحت عمود
+
+356
+00:32:53,650 --> 00:32:57,070
+الكهربا في أيام الامتحانات و يصير يجرأ في الليل
+
+357
+00:32:57,070 --> 00:33:01,530
+على الضوءمش فيه عمدان كهربا عليها ضوء يقعد يجرى
+
+358
+00:33:01,530 --> 00:33:05,930
+مسكين فالدنيا أربانية و فيه امتحانات جاء تحتها
+
+359
+00:33:05,930 --> 00:33:11,770
+بالعمود صبح لجأ زميله متوفي تحت العمود فجال يا
+
+360
+00:33:11,770 --> 00:33:15,690
+مسكيني انا والله قصرت في صاحبي اصول أخدته عندي يا
+
+361
+00:33:15,690 --> 00:33:21,010
+جارية يجرى في دارنا فبمعنى انه ممكن البرد يدى الى
+
+362
+00:33:21,010 --> 00:33:23,750
+الى وفاة نعم
+
+363
+00:33:26,960 --> 00:33:33,740
+كيف بنسف عضة التلج الوقاية
+
+364
+00:33:33,740 --> 00:33:38,380
+خيره من العجل الوقاية خيره من العجل وين في منطقة
+
+365
+00:33:38,380 --> 00:33:45,340
+ممكن تعمل تلج او عض التلج او منطقة باردة جدا الاصل
+
+366
+00:33:45,340 --> 00:33:48,980
+مااتعرضش إلها الحاجة التانية هو لازم ألبس لبس
+
+367
+00:33:48,980 --> 00:33:54,640
+كفاية انا .. انا بشوف الناس الأجانب مثلا تبقى
+
+368
+00:33:54,640 --> 00:33:58,860
+لوحدهافي الأربانية و لابس اللباس خفيف جدا يعني
+
+369
+00:33:58,860 --> 00:34:04,540
+احنا عندنا في الصيف بنحس بسجعة فليش هيك لأن
+
+370
+00:34:04,540 --> 00:34:11,560
+الجماعة عندهم بيشرب و الشرب هذا بيعطي إحساس زائف
+
+371
+00:34:11,560 --> 00:34:17,640
+بالدفع أخدين بالكواكيف لكن بعد شوية اتعرض جسمها
+
+372
+00:34:17,640 --> 00:34:23,340
+لرفعية البرد الشديد لازم نغطي الجسم المظاب و نبعده
+
+373
+00:34:23,340 --> 00:34:30,650
+عن منطقة البردو نسجيله سائل دافى يعنى بدنا نعمله
+
+374
+00:34:30,650 --> 00:34:35,690
+تدفئة خارجية بالغطا ايه وقت فاكره اذا وجدت ولد
+
+375
+00:34:35,690 --> 00:34:41,350
+انبلل و لابس أوعى تدفى جد ما تدفى بياخد وجه طويل
+
+376
+00:34:41,350 --> 00:34:45,230
+جدا لما يدفع اول حاجة بدك تسويها جيه المبلول
+
+377
+00:34:45,230 --> 00:34:52,170
+غيريله اياه ولفىه بجمعش دافى ناشف لأن ان ضال الجسم
+
+378
+00:34:52,170 --> 00:34:57,390
+انبلل باخد وجه طويل جدالما نيدفع فبنجيب الجزء اللى
+
+379
+00:34:57,390 --> 00:35:02,590
+مبالغ لهذا و بنلبسه بقواعى جافة و بندفعه و بنبدأ
+
+380
+00:35:02,590 --> 00:35:10,450
+تدفعه بالتدريج يعني ممكن نحط ميه دافية نسجيه شيء
+
+381
+00:35:10,450 --> 00:35:15,040
+سخنماما تيجي تسجي كباية شاي سخنة أو كباية حليب
+
+382
+00:35:15,040 --> 00:35:21,700
+سخنة او اي مشروب سخن حتى مراجة دافية بتلاقي بيصير
+
+383
+00:35:21,700 --> 00:35:27,580
+تدفع داخلية بالاضافة لتدفع الخارجية مابنحطش دفيات
+
+384
+00:35:27,580 --> 00:35:32,840
+مباشرة ماتجيبيش دفيات مباشرة تحطيها على الإنسان
+
+385
+00:35:32,840 --> 00:35:37,880
+ليش؟ انا نفسي بتعرفي الدافع مش هيش مفروض اسفل
+
+386
+00:35:37,880 --> 00:35:38,360
+المطار
+
+387
+00:35:41,740 --> 00:35:44,940
+ذاكرين احنا قولنا ايش؟ يحس الإنسان في أول الأمر
+
+388
+00:35:44,940 --> 00:35:49,780
+بألم بسيط قد لا يلاحظوا البعض ولكن بعد ذلك ينعدم
+
+389
+00:35:49,780 --> 00:35:55,240
+الإحساس بالألم ماما صار مافيش إحساس وحطيت دفاية
+
+390
+00:35:55,240 --> 00:36:03,160
+وانحرج ماحسش يعني في كان لي نصيب كان بيجرى في
+
+391
+00:36:03,160 --> 00:36:09,160
+بيرزيت بيرزيت هذه منطقة تلج يعني بيخرفونا كأنه
+
+392
+00:36:09,160 --> 00:36:14,070
+يعني الحنفيةاذا كان بتنقط مثلا بيقوم الصبح بيلاقوا
+
+393
+00:36:14,070 --> 00:36:20,990
+ال .. من الأرض لحنفية خط تلج، الحنفية بيفتح و لا
+
+394
+00:36:20,990 --> 00:36:26,910
+بتطيهش، من أيش؟ شدة البلودة، فهذا نصيبي كان من
+
+395
+00:36:26,910 --> 00:36:30,390
+الأوائل، من الأوائل على القطار و راح يجرح في BHZ
+
+396
+00:36:30,390 --> 00:36:35,270
+تهندسة، كل ما بغى يجي أروح أسلم عليه ألاقي بجامته
+
+397
+00:36:35,270 --> 00:36:40,990
+محروقةاجر البجاما من تحت محرجات اول مرة لبسها اول
+
+398
+00:36:40,990 --> 00:36:44,290
+بجاما محرجة تاني بجاما لبسها اللي هي محرجة بقوللي
+
+399
+00:36:44,290 --> 00:36:48,030
+ورا كده الشياطين قاللي والله انت عارف عشان هناك
+
+400
+00:36:48,030 --> 00:36:54,970
+ثلج و سجعة بحط التل الدفاية و بحط اجري فوقها بحسش
+
+401
+00:36:54,970 --> 00:37:00,810
+في الدفاية انها موجودة الا لما اشم ريحة الدخنة
+
+402
+00:37:00,810 --> 00:37:07,440
+اللي بتطلع من البنطدون ايش بنفهمإن مافيش إحساس لما
+
+403
+00:37:07,440 --> 00:37:11,480
+يصير فيه برد لما يصير فيه قضات برد طبعا هو بده
+
+404
+00:37:11,480 --> 00:37:14,800
+يصهر و يجرى عشان يحصل مجموع يعني و عشان ينجح
+
+405
+00:37:14,800 --> 00:37:19,260
+ماخدين بقى الكوكب؟ فإذا بجيبش أنا دفاية جنب البني
+
+406
+00:37:19,260 --> 00:37:25,400
+آدم و بخليه يتدفى مباشر و أنما ممكن جدا يعني غرفة
+
+407
+00:37:25,400 --> 00:37:28,440
+مثلا تلاتة في أربعة أو أربعة في أربعة أحط فيها
+
+408
+00:37:28,440 --> 00:37:32,400
+دفاية قعدة جنب بعيد مترين عن البني آدم بحمل جو
+
+409
+00:37:32,400 --> 00:37:36,380
+بالإضافة إلى الغطاةبالاضافة إلى التدفئة الداخلية،
+
+410
+00:37:36,380 --> 00:37:40,620
+اللي هي عبارة عن أيش التدفئة الداخلية؟ مسجين، حاجة
+
+411
+00:37:40,620 --> 00:37:44,780
+سخنة، التدفئة الخارجية هي الملابس، مظبوط؟ بس
+
+412
+00:37:44,780 --> 00:37:50,180
+الداخلية عبارة عن أيش؟ مشروب ساخن، بيصير عنده أيش؟
+
+413
+00:37:50,180 --> 00:37:55,720
+دفة، الجزء المصاب بندلكوش مطلقا، طب ليش الجزء
+
+414
+00:37:55,720 --> 00:37:59,060
+المصاب بندلكوش؟
+
+415
+00:38:03,620 --> 00:38:12,200
+بصير مشكلة ممكن يصير مشكلة اه ليش؟ اه ليش طيب؟ لأن
+
+416
+00:38:12,200 --> 00:38:16,540
+ايش اللي صار في الجزء اللي صار البلورات التلجية
+
+417
+00:38:16,540 --> 00:38:20,640
+اتكونت تحت الحين البلورات التلجية لما نبدأ ندفي
+
+418
+00:38:20,640 --> 00:38:24,960
+لما تفكي التلاجة اللي الفريزر ملان تلج ايش بيصير
+
+419
+00:38:24,960 --> 00:38:31,160
+فيه؟ نعم بيبدأ يسيح و بيبدأ يصير زي القبر زي
+
+420
+00:38:31,160 --> 00:38:36,540
+مساميرلما التلج فى الإيد أو فى الرجل يبدأ يسيح
+
+421
+00:38:36,540 --> 00:38:41,240
+بيصير بوزه زى المسامير بتيجى تدلقيه شو بيساوي؟
+
+422
+00:38:41,240 --> 00:38:45,720
+بيخرب العنسجة ومبوت الجلد بيخرب العنسجة ومبوت
+
+423
+00:38:45,720 --> 00:38:50,700
+الجلد وبناء عليه مانعملش عمل تدليق للمنطقة المصابة
+
+424
+00:38:50,700 --> 00:38:57,920
+لأن هذا ممكن يعمل تلف للعنسجة مانعملش
+
+425
+00:38:57,920 --> 00:39:02,500
+انصار أحيانا بيصير فقاعات تلجيةزي ما الحرق من
+
+426
+00:39:02,500 --> 00:39:08,260
+الدرج التاني بعمل فقاعات في الحرق أو عضة التلج
+
+427
+00:39:08,260 --> 00:39:14,020
+بعمل فقاعات فيها بلورات تلجية هذه برضه بنحاولش
+
+428
+00:39:14,020 --> 00:39:21,320
+نفكها ولا نفركها ولا نحركها وطبعا التلجبيعمل ورم
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+429
+00:39:21,320 --> 00:39:26,620
+فعشان مايصيرش ورم زيادة بدي أرفع إيدين ورجلين
+
+430
+00:39:26,620 --> 00:39:31,660
+المصاب أعلى من مستوى القلب عشان يخف الورم ومايصيرش
+
+431
+00:39:31,660 --> 00:39:37,000
+انتفاخ كتير من الصبايا يمكن بتلاقيها مجرد ماتيجي
+
+432
+00:39:37,000 --> 00:39:42,600
+أرباني تشتاء صابع إيديها ورجليها بيرموا بيرموا و
+
+433
+00:39:42,600 --> 00:39:45,540
+بتظل طول الشتاء ومناخيرها بيكبرن
+
+434
+00:39:48,520 --> 00:39:54,020
+جد اه بيصيرون ذاكمين ع طول مضال كل فترة الشتاء ايش
+
+435
+00:39:54,020 --> 00:39:59,620
+السبب في هذا الكلام السبب في هذا الكلام انه هي
+
+436
+00:39:59,620 --> 00:40:03,720
+استنت على حالها لما صار في تلف خلايا في إيديها و
+
+437
+00:40:03,720 --> 00:40:10,140
+في رجليها تلفت و بعدين أجت تصحى اتدف حالها اللي
+
+438
+00:40:10,140 --> 00:40:14,060
+بيصير معاها زي هيك انا بانصحها ايش تعمل تستقبل
+
+439
+00:40:14,060 --> 00:40:19,240
+الشتاء بدري يعني ماتستناش على أطرافها يرمنو بعدين
+
+440
+00:40:19,240 --> 00:40:24,920
+تبدأ تهتم انهم يعني تدفّيهم لأ تدفّيهم من بدري قبل
+
+441
+00:40:24,920 --> 00:40:29,960
+أن يصير السجعة مظبوط تبدأ تلبس .. لبس شتاء و إذا
+
+442
+00:40:29,960 --> 00:40:36,580
+في إمكانية تلبس كفات في الإيديان و في الرجلين يبقى
+
+443
+00:40:36,580 --> 00:40:42,520
+تدفّي الظهر بيصير معاكن دي بالظبط من بدري شتّي
+
+444
+00:40:42,520 --> 00:40:48,150
+البس دافع من بدريو تحاوليش تتعرض للبرد عشان
+
+445
+00:40:48,150 --> 00:40:51,870
+مايرموش أديك أصلا لأنه إذا ورموا بيرضوش يطيبوا إلا
+
+446
+00:40:51,870 --> 00:40:55,570
+لما يخلص الشتاء قرابس يعني بتقعدين لتالتة اربعة
+
+447
+00:40:55,570 --> 00:41:02,030
+أشهر للحكاية و هي مشكلة فيرفع الجزء المصاب فوق
+
+448
+00:41:02,030 --> 00:41:08,010
+مستوى القلب عشان مايصيرش ورم و يصير الوضع كويس
+
+449
+00:41:08,010 --> 00:41:11,170
+ماشي؟
+
+450
+00:41:11,170 --> 00:41:19,970
+غايبوه بقى؟قولنا ايش سببها اهم سبب اصابة الرأس
+
+451
+00:41:19,970 --> 00:41:27,870
+الرأس فيها ايش المخ مركز العصاب فغالبا الغيبوبة
+
+452
+00:41:27,870 --> 00:41:34,650
+بتسير من تأثر جهاز العصبي اللي هو متمثل بالمخ ماشي
+
+453
+00:41:34,650 --> 00:41:42,590
+ايش بسبب هذه الاصابات ضرب في المخ ضرب تدمب سواء
+
+454
+00:41:42,590 --> 00:41:48,140
+يعني نزيففي داخل الجمجمة او في اعلى المخ بين
+
+455
+00:41:48,140 --> 00:41:54,620
+العظمة الجمجمة وبين المخ ممكن يصير نزيف بيعمل ما
+
+456
+00:41:54,620 --> 00:42:02,580
+يسبب الغيبوبة ايش بيسبب هذا الشيء ضرب حادث سيارة
+
+457
+00:42:02,580 --> 00:42:11,100
+سقوط شجار الرياضة العنيفة كل هذه الشغلات اي إصابة
+
+458
+00:42:11,100 --> 00:42:16,480
+قوية ممكن تعملاصابة في الراس و اتسبب الغيبوبة انا
+
+459
+00:42:16,480 --> 00:42:22,060
+اعرف طفل كان في المستشفى لما روحت انا على المكان
+
+460
+00:42:22,060 --> 00:42:26,120
+اللي هو فيه كان له شهرين في حالة غيبوبة طفل هذا من
+
+461
+00:42:26,120 --> 00:42:30,800
+الشجاعية فبسأل عشان اعرف ايش الحدث و نتعامل معاه
+
+462
+00:42:30,800 --> 00:42:33,840
+قالوا هذا كان بيطير طيارة اللي بيطيروا فيها
+
+463
+00:42:33,840 --> 00:42:38,620
+الأطفال و هو بيطير فيها حجر تحت رجله وجع اللي هو
+
+464
+00:42:38,620 --> 00:42:47,550
+ضارب في راسه فانضرب المخيخالمخيخ انضرب ايام شفته
+
+465
+00:42:47,550 --> 00:42:52,930
+كان له شهرين في حالة غيبوبة طبعا احنا خلصنا و سبنا
+
+466
+00:42:52,930 --> 00:42:57,690
+و الله بعلم ماكنتش صحي صحي و ماصحيش فأي إصابة في
+
+467
+00:42:57,690 --> 00:43:03,110
+المخ ممكن تسبب ما يسمى الغيبوبة نعم ايش إصابة
+
+468
+00:43:03,110 --> 00:43:08,270
+بالرقص؟ قاعدين بقول إصابات الرقص لأ يعني إيش ال ..
+
+469
+00:43:08,270 --> 00:43:12,790
+مثلا جيت أنا عند أخر كتير بوم عليهابس يعني ..
+
+470
+00:43:12,790 --> 00:43:18,870
+هنحكي عنه على الإغناق و التشنجات تصير تشنجات معاها
+
+471
+00:43:18,870 --> 00:43:24,390
+تتشنج التشنجات ان شاء الله هنحكي عليها فالغيبوبة
+
+472
+00:43:24,390 --> 00:43:30,970
+من أسباب الغيبوبة اللي هي الإصابات في الرأس الحاجة
+
+473
+00:43:30,970 --> 00:43:36,310
+التانية أمراض الجهاز العصبي أمراض الجهاز العصبي
+
+474
+00:43:36,310 --> 00:43:43,230
+إيش عن أمراض الجهاز العصبي تسمع أختك أنتيصار عندها
+
+475
+00:43:43,230 --> 00:43:47,070
+حمّة شوكية و هي صغيرة نامت المستشفى يعني و هي
+
+476
+00:43:47,070 --> 00:43:52,170
+صغيرة لو صار عند الإنسان مرض في الجهاز العصبي زي
+
+477
+00:43:52,170 --> 00:44:00,850
+مثلا .. اه .. لو صار عند الواحد حمّة شوكية مثلا
+
+478
+00:44:00,850 --> 00:44:04,690
+الحمّة الشوكية اللي هي التهاب السحاية ما هي
+
+479
+00:44:04,690 --> 00:44:10,710
+السحاية السحاية هي أغشية المخلما تلتهب أغشية المخ
+
+480
+00:44:10,710 --> 00:44:15,310
+تلتهب بنتجر على خلايا المخ بيأثر على الإنسان و
+
+481
+00:44:15,310 --> 00:44:21,150
+ممكن يعمل ما يسمى بالغيبوبة فكلها شغلات اللي علاقة
+
+482
+00:44:21,150 --> 00:44:28,550
+بإيش بالمخ فإذا أمراض الجهاز العصبي أي انسداد في
+
+483
+00:44:28,550 --> 00:44:35,450
+الشراين أو جلطة نزيف في المخ ممكن يعمل ما يسمى
+
+484
+00:44:35,450 --> 00:44:40,870
+بالغيبوبةفي حاجة اسمها الصرق الكبير و حاجة اسمها
+
+485
+00:44:40,870 --> 00:44:46,470
+الصرق الصغير الصرق اللي الأخت قاعدة بتحكي عنه
+
+486
+00:44:46,470 --> 00:44:52,750
+الصرق الكبير اللي هو بيسير حركات لا إرادية بتؤدي
+
+487
+00:44:52,750 --> 00:44:57,670
+إلى غيبوبة كثير من الناس بيقولك سببها أنه في
+
+488
+00:44:57,670 --> 00:45:03,810
+كهربزايدة في المخ عملت رسم مخ عملت رسم في المخ
+
+489
+00:45:08,010 --> 00:45:10,970
+طب ماشي احنا بدنا نعرف ليش يغم عليها بدنا نروح
+
+490
+00:45:10,970 --> 00:45:16,170
+نصور مخها بتروح على مستشفى الأمراض العصبية اللى
+
+491
+00:45:16,170 --> 00:45:20,770
+جنب مستشفى العيون و بتقضيها على أطباء الجهاز
+
+492
+00:45:20,770 --> 00:45:27,410
+العصبي هناك و بشوفوا إذا كان في مشكلة في الكهربامش
+
+493
+00:45:27,410 --> 00:45:31,250
+لأنه لما ضم عليها المستشفى كانت قدامها ضييف وشغل
+
+494
+00:45:31,250 --> 00:45:34,030
+ومن غير ما توديها على المستشفى؟ بظبط يا الشيخ الله
+
+495
+00:45:34,030 --> 00:45:37,490
+يرضى عليك هذه كل ما غم عليها أكتر كل ما غمت أكتر
+
+496
+00:45:37,490 --> 00:45:42,990
+كل ما صار وضعها أسوأ فبدها علاج ضروري جدا ناشي
+
+497
+00:45:42,990 --> 00:45:48,190
+فتوديها على مستشفى الأمراض العصبية أو على عيادة
+
+498
+00:45:48,190 --> 00:45:56,500
+الصراج اللي هي عرفها عيادة الصراج بعد طبع العصبجنب
+
+499
+00:45:56,500 --> 00:45:59,760
+العيون بالظبط هي هذه المطلوبة جنب العيون اللي هي
+
+500
+00:45:59,760 --> 00:46:06,760
+جسم الأصابع اذا صارت الجهاز التنفسي الجهاز عصبي
+
+501
+00:46:06,760 --> 00:46:12,780
+عفونا انسداد في شرايين المخ حم شوكية في حاجة اسمها
+
+502
+00:46:12,780 --> 00:46:19,300
+صرع كبير صرع كبير غالبا غالبا بيقولوا انه سببه غير
+
+503
+00:46:19,300 --> 00:46:23,040
+معروف الا انه بيصوروا البني ادم بيلاجوا فيه كهره
+
+504
+00:46:23,040 --> 00:46:29,530
+بزيادةمخنا مش بنعمل حاجة اسمها تخطيط قلب، ايش عن
+
+505
+00:46:29,530 --> 00:46:36,210
+تخطيط قلب؟ تخطيط القلب رسم كهربية القلب، جهاز بلسم
+
+506
+00:46:36,210 --> 00:46:40,510
+كهربة القلب، في تخطيط دماغ، تخطيط دماغ رسم كهربة
+
+507
+00:46:40,510 --> 00:46:44,090
+الدماغ، فإن زادت الكهربا في الدماغ عند بعض الناس،
+
+508
+00:46:44,090 --> 00:46:48,350
+عند معظم الناس، بيصير إيش؟ ما يسمى بالصراع، طبعا
+
+509
+00:46:48,350 --> 00:46:51,530
+هذا بيوقد علاج، تعرفوا أنتوا المرة اللي أجت للنبي
+
+510
+00:46:51,530 --> 00:46:53,890
+صلى الله عليه وسلم و قالتلي يا رسول الله اني أسرع
+
+511
+00:46:54,530 --> 00:46:58,350
+النبي إيش قال لها؟
+
+512
+00:46:58,350 --> 00:47:02,790
+ادعو الله ليه أني أشفيني قال لها إيش؟ إن شئتي دعوت
+
+513
+00:47:02,790 --> 00:47:08,550
+لكي و شفاك الله و إن شئتي صبرتي طبعا العاجل إيش
+
+514
+00:47:08,550 --> 00:47:14,070
+هيقول؟ بده تقطعوا روح الجنة، مظبوط؟ الناس كلها
+
+515
+00:47:14,070 --> 00:47:19,340
+بتضحي بكل ما تملك عشان إيش؟عشان الجنة فقالت لا
+
+516
+00:47:19,340 --> 00:47:23,100
+اصبر وليك الجنة بس يا رسول الله اني اتكشف فدعو
+
+517
+00:47:23,100 --> 00:47:27,720
+الله ليه قال اتكشف ادعالها انا بفهم من هذا الحديث
+
+518
+00:47:27,720 --> 00:47:32,800
+ان النبي صلى الله عليه وسلم قالها مافيش إليك عداجة
+
+519
+00:47:32,800 --> 00:47:38,800
+هي قالها مرة عنكوا حد أجى شكل النبي من إشي وقالله
+
+520
+00:47:38,800 --> 00:47:44,920
+اصبر وليك الجنة غير هادىحد مر عنكوا ان النبي قالوا
+
+521
+00:47:44,920 --> 00:47:48,440
+اصبر ولك الجنة غير هادى طبعا الناس اللى بيفخدوا
+
+522
+00:47:48,440 --> 00:47:53,400
+عديهم هذا اش تانى يعنى فش اللي هادى كأنه قال للنبي
+
+523
+00:47:53,400 --> 00:47:58,140
+انت مالكيش علاج فاصبري ولكي الجنة و لحد اليوم فش
+
+524
+00:47:58,140 --> 00:48:04,160
+علاج للصراع انما ايش بينعملهم هذول الناس بيعطوا
+
+525
+00:48:04,160 --> 00:48:10,640
+علاج يخفف نوبة الصراعو يباعل المسافة بين النوبة
+
+526
+00:48:10,640 --> 00:48:14,520
+الأخرى بدل ما يجيها صرع كل يوم الحالة أو كل شهر
+
+527
+00:48:14,520 --> 00:48:18,520
+بيصير بدل كل شهر يجي كل ستة أشهر و بدل ما تيجي
+
+528
+00:48:18,520 --> 00:48:23,360
+حالة شديدة و تشد خالص تيجي حالة أخف شوية هذه
+
+529
+00:48:23,360 --> 00:48:27,960
+بالظبط اللي ممكن ينعمل لهيك ناس اللي هم تبعين
+
+530
+00:48:27,960 --> 00:48:32,280
+الصرع الصرع طبعا هذا بسبب غيبوبة الصرع الصغير إيش
+
+531
+00:48:32,280 --> 00:48:36,540
+بيصير الصرع الصغير بيصير جزء من الجسم
+
+532
+00:48:39,080 --> 00:48:44,360
+بشد يعني انا شوفت حدا بنبقى .. بنبقى قاعد معايا
+
+533
+00:48:44,360 --> 00:48:48,340
+ولا اتخرف انا وياه وحكي ان انا واحنا قاعدين ثوانى
+
+534
+00:48:48,340 --> 00:48:55,960
+كان عامل زيه وصحي وماعوش خبر هذا اسمه صراع صغير
+
+535
+00:48:55,960 --> 00:48:59,720
+لكن الصراع القبيل بتمدد و بتشنج و كل جسمه بيصير
+
+536
+00:48:59,720 --> 00:49:06,320
+ايه اي رجش هذا اسمه الصراعفي عندنا حاجة اسمها
+
+537
+00:49:06,320 --> 00:49:11,880
+غيبوبة نقص السكر وفي حاجة اسمة غيبوبة زيادة السكر
+
+538
+00:49:11,880 --> 00:49:17,020
+طبعا مين أخطر فيهم؟ غيبوبة نقص السكر ليش قلنا
+
+539
+00:49:17,020 --> 00:49:20,360
+أخطر؟
+
+540
+00:49:20,360 --> 00:49:27,960
+المخ بتغذى على السكر و لو نقص التغذى على المخ ممكن
+
+541
+00:49:27,960 --> 00:49:34,020
+يتأثر على خلايا المخ و تضربو تخرب خالص تعتل و يخش
+
+542
+00:49:34,020 --> 00:49:40,200
+الإنسان في عاهة مستديم فعشان هيك إذا جيتي أنت
+
+543
+00:49:40,200 --> 00:49:45,240
+ماعرفتيش تقرري أن هذه حالة نقص السكر أو زيادة
+
+544
+00:49:45,240 --> 00:49:49,500
+السكر و بدك تتعامل معها إنها نقص السكر حكيتلكوا
+
+545
+00:49:49,500 --> 00:49:55,580
+قصة المرأة اللي جيت و قالوا بدها تموت؟ اه؟ ضلينا
+
+546
+00:49:55,580 --> 00:50:00,180
+نطعميها سكر لما صار بعد مزهجنا و احنا نطعميها صار
+
+547
+00:50:00,180 --> 00:50:03,810
+السكر سبعينماخدين بقى الكوكيف؟ كدهش بقى جهازه في
+
+548
+00:50:03,810 --> 00:50:10,310
+الأول فنقص السكر غيبوته أكثر خطورة من زيادة السكر
+
+549
+00:50:10,310 --> 00:50:14,070
+وإذا لم تستطيع التمييز بينهم حكينا فيه حاجات
+
+550
+00:50:14,070 --> 00:50:17,810
+أساسية للتمييز بينهم قلنا إن زيادة السكر بيكون فيه
+
+551
+00:50:17,810 --> 00:50:23,470
+ريحة زي ريحة التفاح التالف أو الاسيتون من ريحة
+
+552
+00:50:23,470 --> 00:50:28,990
+النفس بسبب زيادة السكر وقلنا إن الناس اللي نقص سكر
+
+553
+00:50:28,990 --> 00:50:36,130
+بيبقى متوتر وعرجان وعنيمالبيو .. الحدقة بتختلف
+
+554
+00:50:36,130 --> 00:50:43,130
+شكلها و .. و رايج و مش مجمع كل هذه الشغلات و بكون
+
+555
+00:50:43,130 --> 00:50:49,290
+إنسان مهتم جدا في أخد علاج السكري لكن باكلش باخد
+
+556
+00:50:49,290 --> 00:50:54,250
+العلاج و باخدش إيش طعام فبظل العلاج يقض من سكره
+
+557
+00:50:54,250 --> 00:50:59,670
+لما يصل إلى أن السكر ينزل فإذا أنت جيت و ماعرفتش
+
+558
+00:50:59,670 --> 00:51:05,190
+تميزي تعتبر أنه حالةنقص سكر وبتبدي تعطيه سكر ليش
+
+559
+00:51:05,190 --> 00:51:09,790
+جد ما تعطيه يعني سكر هيزيد عشر او خمستاشر او عشرين
+
+560
+00:51:09,790 --> 00:51:12,750
+اذا كان خمسمية وصار خمسمية وعشرين مش كتير بختلف
+
+561
+00:51:12,750 --> 00:51:17,650
+لكن لو كان اربعين ونقصت سكره لعشرين ممكن يموت او
+
+562
+00:51:17,650 --> 00:51:23,630
+ممكن يصير في عنده تلف في خلايا دماغ اتلازمه باقي
+
+563
+00:51:23,630 --> 00:51:30,230
+عمره وعليه ايش بيصير حالته صعبة اذا احنا اذا لم
+
+564
+00:51:30,230 --> 00:51:35,450
+نستطع ان نحددبنروح ندى شيء بواسطة الفم إذا كان
+
+565
+00:51:35,450 --> 00:51:44,410
+المريض عنده قابلية لإنه ياخد حاجة بالفم في
+
+566
+00:51:44,410 --> 00:51:50,750
+عندنا الأشياء اللى بتسبب كمان الغيبوبة اللى هى
+
+567
+00:51:50,750 --> 00:51:58,790
+المراحل النهائية لتلف الكبد و تسمم الحملالمراحل
+
+568
+00:51:58,790 --> 00:52:01,210
+النهائية اللي حصل في الكبد عمرك شوفت واحد عنده
+
+569
+00:52:01,210 --> 00:52:05,430
+مشكلة في الكبد الناس اللي بيبقى عنهم صفر صفر و
+
+570
+00:52:05,430 --> 00:52:12,350
+بيبقى شكله اسمه شكل صدره زي البرميل شكل صدره ملفوف
+
+571
+00:52:12,350 --> 00:52:18,650
+و هو ناشف زي العودة جريودية لكن صدره ضخم في تجمع
+
+572
+00:52:18,650 --> 00:52:24,690
+سوائل و ماعي في الفترة هذه بتلاقي عنه صفر صفروجلده
+
+573
+00:52:24,690 --> 00:52:28,850
+أصفر أصفر، إيش يعني؟ يعني مادة ال .. المادة
+
+574
+00:52:28,850 --> 00:52:33,610
+الصفراء اللي المفروض تطلع من الجسم متجمعة، هذه
+
+575
+00:52:33,610 --> 00:52:38,590
+تعتبر سم على المخ هذه تعتبر سم، أمرا كنت سمعت أن
+
+576
+00:52:38,590 --> 00:52:43,890
+واحد دمها سالب ومتجوز واحد دمه موجب، إيش بيعملوا
+
+577
+00:52:43,890 --> 00:52:49,030
+لهم ولادهم؟ أنت دمك سالب، متجوز واحد دمه موجب، إيش
+
+578
+00:52:49,030 --> 00:52:53,560
+بيساوي .. بيساوي كل بطن؟إيش بتسوي كل متلة دى؟
+
+579
+00:52:53,560 --> 00:52:57,340
+بيفحصوا دمات إذا دم بنت زيه ماعطلوش إبرا بس إذا
+
+580
+00:52:57,340 --> 00:53:03,680
+دمها بفتلت موجب ماعطلوش إبرا بالظبط بالظبط فإن طلع
+
+581
+00:53:03,680 --> 00:53:10,160
+دم الجنين سالب زي أمه مافيش مشكلة لكن طلع موجب و
+
+582
+00:53:10,160 --> 00:53:16,000
+جاء جزء من دمه على الأم بتسير المرة الجاية كل حمل
+
+583
+00:53:16,000 --> 00:53:16,940
+مشكلة
+
+584
+00:53:19,650 --> 00:53:25,710
+لأن في صار أجسام غريبة في الجسم أجسام .. أه بيغلب
+
+585
+00:53:25,710 --> 00:53:31,930
+ماخدين بالكوفة فهدولة تبعين الكبد الناس اللي زي
+
+586
+00:53:31,930 --> 00:53:37,090
+هيك ممكن يخشوا في مشاكل اتجمع
+
+587
+00:53:37,090 --> 00:53:40,770
+العصارة الصفروية على المخ بيصير وجههم أصفر أصفر
+
+588
+00:53:40,770 --> 00:53:46,630
+ويصير تسمم في المخ ويقدر إلى غيبوبة كمان في تلف
+
+589
+00:53:46,630 --> 00:53:51,050
+الكبد بيصير زي هيك بالظبط مع الكبارفبصير عندهم
+
+590
+00:53:51,050 --> 00:53:56,070
+تسمم من تلف الكبد المنومات
+
+591
+00:53:56,070 --> 00:54:03,230
+المنومات واحد ممكن يشرب منوم لما يصير مافيش
+
+592
+00:54:03,230 --> 00:54:08,650
+استجابة في المخ الخمر ايش الخمر تعريفها كل ما خمر
+
+593
+00:54:08,650 --> 00:54:13,510
+العقل خمر العقل يعني يبطل العقل يستجيب احنا كيف
+
+594
+00:54:13,510 --> 00:54:19,330
+بنتعامل مع مخنا بيكون صاحيلكن لو العقل بطل يستجيب
+
+595
+00:54:19,330 --> 00:54:23,550
+يخش الواحد في غيبوبة و في نوم و في حاجات زي هيك و
+
+596
+00:54:23,550 --> 00:54:26,830
+طبعا يعني في بعض الحالات بتموت تصور الواحد لو ما
+
+597
+00:54:26,830 --> 00:54:32,190
+يكون خاتمته يموت سكران نصيب الله العفو و العافية
+
+598
+00:54:32,190 --> 00:54:37,650
+اللي بيشربوا الأثيون و الحشيش و الكحول الناس اللي
+
+599
+00:54:37,650 --> 00:54:43,290
+بيشربوا يعني السم من المبيضات الحشرية برضهبيعمل
+
+600
+00:54:43,290 --> 00:54:47,810
+تأثير على الدم وبيأثر على المخ وبيعمل ما يسمى
+
+601
+00:54:47,810 --> 00:54:54,190
+بالغيبوبة عشان نعرف احنا ايش سبب الغيبوبة احسن
+
+602
+00:54:54,190 --> 00:54:57,550
+واحد يقولنا معلومات اللي هو المريض او المصاب
+
+603
+00:54:57,550 --> 00:55:05,970
+المصاب مش صاحي اللي حواليه انا كمصحف باجي بطلع اذا
+
+604
+00:55:05,970 --> 00:55:10,510
+والله في علامة زيادة السكر او نقص السكر باعطيله
+
+605
+00:55:10,940 --> 00:55:14,940
+علاج لزيادة السكر او نقص السكر اذا ماكنتش احدد
+
+606
+00:55:14,940 --> 00:55:22,340
+باعتبره ايش نقص سكر وبديله سكر وباقى الحالات غالبا
+
+607
+00:55:22,340 --> 00:55:28,860
+بنوديها على اقرب مركز طبي ناشي الحال هذا بالنسبة
+
+608
+00:55:28,860 --> 00:55:38,240
+للغايمون الحين بدنا نمشي على الاصابات الجهاز
+
+609
+00:55:38,240 --> 00:55:45,160
+التنفسيلو سألناك سؤال غريب، مين عارف كيف بتتنفس؟
+
+610
+00:55:45,160 --> 00:55:48,760
+شهيق و زفير، مين عارف كيف بيصير دخل أكسجين و
+
+611
+00:55:48,760 --> 00:55:52,380
+طلعتين أكسجين كالبون؟ مين عارف كيف ميكانيكية
+
+612
+00:55:52,380 --> 00:56:01,340
+العملية بتتم؟ بتنقبض و بتنبسط الرقتين، كيف؟
+
+613
+00:56:01,340 --> 00:56:06,020
+اما هو كيف بتتعب بالهواء؟ يعني ليش انا مثلا مايتعب
+
+614
+00:56:06,020 --> 00:56:12,070
+اندناي بالهواء بدل من الرقتين؟ايش اللي بيصير؟
+
+615
+00:56:12,070 --> 00:56:19,170
+إشارة من المخ ايش بتقول طيب
+
+616
+00:56:19,170 --> 00:56:23,490
+صدقوا
+
+617
+00:56:23,490 --> 00:56:30,150
+كله هذا أخدته بس بالظبط
+
+618
+00:56:30,150 --> 00:56:36,810
+بالظبط كيف أنا بتنفس هاي الكلام بتتنفس سن أكتر من
+
+619
+00:56:36,810 --> 00:56:42,820
+عشر سنينعمرك سألت الحقيقة كيف تتنفسى؟ احنا بنتنفس
+
+620
+00:56:42,820 --> 00:56:46,420
+كالقاتل المخ
+
+621
+00:56:46,420 --> 00:56:53,180
+مهم جدا فى الجهاز التنفسى المخ بيقدى اشارة للجسم
+
+622
+00:56:53,180 --> 00:56:58,420
+او للجهاز التنفسى انه خص عليك اكسوجين الجسم عايز
+
+623
+00:56:58,420 --> 00:57:04,520
+اكسوجين فالاكسوجين وين موجود؟ احنا عيمين فيه في
+
+624
+00:57:04,520 --> 00:57:09,250
+وسطه .. في وسطه احنافبروح بيأدي إشارة للجهاز
+
+625
+00:57:09,250 --> 00:57:15,650
+التنفسي الجهاز التنفسي بتقبض الحجاب الحاجز الحجاب
+
+626
+00:57:15,650 --> 00:57:21,670
+الحاجز لما انقبض شكل الحجاب الحاجز كالقاعدة هيك
+
+627
+00:57:21,670 --> 00:57:26,650
+لما
+
+628
+00:57:26,650 --> 00:57:33,670
+انقبض الحجاب الحاجز سيرهان ايش بيصير بزيد حجم
+
+629
+00:57:33,670 --> 00:57:38,630
+الصدرمظبوط؟ لما يزيد حجم الصدر إيش بيصير؟ بيصير
+
+630
+00:57:38,630 --> 00:57:44,670
+هوا سالب، في نقص هوا جوا بيصير، بده تعباية، من أين
+
+631
+00:57:44,670 --> 00:57:50,990
+بتعباه؟ من الأنف و الفم، تخش هوا من الأنف و الفم،
+
+632
+00:57:50,990 --> 00:57:56,210
+كمان من الأشياء اللي بتصير عشان أنا أتنفس، عمرك
+
+633
+00:57:56,210 --> 00:58:02,510
+شفت طفل صغير و هو بتنفس؟ أه؟ فلاج بطنه بطلع و بنزل
+
+634
+00:58:02,510 --> 00:58:08,810
+و صدره بطلع و بنزل، مظبوط؟بين الضلوع الضلوع البني
+
+635
+00:58:08,810 --> 00:58:15,490
+آدم الأضلاء في عضلات و حوالين الضلوع عضلات هذه
+
+636
+00:58:15,490 --> 00:58:21,650
+العضلات بتشد تتشد بتنقبض لما تنقبض شو بيصير بتقدم
+
+637
+00:58:21,650 --> 00:58:25,790
+السدر للأمام و على الجانب فبوسع كمان السدر فبوسع
+
+638
+00:58:25,790 --> 00:58:33,310
+أفقي و رأسي موسى أفقي و رأسي جديش بخس هوا بده
+
+639
+00:58:33,310 --> 00:58:40,240
+تعبايةبخس الهواء فبنزل بداله هواء من الانف والفم
+
+640
+00:58:40,240 --> 00:58:46,460
+او الانف والفم او الفم او الانف بصير دخول الهواء
+
+641
+00:58:46,460 --> 00:58:53,940
+بعادل الضغط طبعا قداش بصير نقص في الهواء بصير
+
+642
+00:58:53,940 --> 00:58:59,340
+حوالي اتنين في المية من ضغط الهواء ناقص اتنين في
+
+643
+00:58:59,340 --> 00:59:04,920
+المية تكفي ان يخش هواء الحين يشبع جسمناأخد محاجته
+
+644
+00:59:04,920 --> 00:59:10,900
+من ال Oxygen المخ بيبعت إشارة الحمد لله شبعنا طب
+
+645
+00:59:10,900 --> 00:59:14,400
+عم تسمعوهاش أنتوا هذه إنما الجهاز التنفس اللي
+
+646
+00:59:14,400 --> 00:59:18,620
+بسمعها من فضل الله سبحانه وتعالى أنه احنا هبنى
+
+647
+00:59:18,620 --> 00:59:23,560
+نتنفس لإرادة تصور لو ان كل نفس بدنا نقوله في بعض
+
+648
+00:59:23,560 --> 00:59:28,300
+الناس لازم يكون يعني صاحي عشان يتنفس أو لازم ياخد
+
+649
+00:59:28,300 --> 00:59:33,350
+علاج معين عشان يعرف يتنفسو لو نسي هذا العلاج ممكن
+
+650
+00:59:33,350 --> 00:59:36,950
+يموت فتصوروا احنا عندنا مشكلة من هذا النوع من فضل
+
+651
+00:59:36,950 --> 00:59:41,470
+الله يعني انه فش فالان المخ اعطى إشارة الحمد لله
+
+652
+00:59:41,470 --> 00:59:46,210
+خلاص أخدنا كفايتنا برجع كل إشي زي ما كان ايش يعني
+
+653
+00:59:46,210 --> 00:59:50,610
+كل إشي برجع زي ما كان؟ الحجاب الحاجز برتخي برجع
+
+654
+00:59:50,610 --> 00:59:56,470
+تاني الصدر برجع لما يرجع الحجاب الحاجز يرجعش الصدر
+
+655
+00:59:56,470 --> 01:00:00,470
+ايش بيصير؟ بضغط تاني الهواء فبصير هواء زيادة جوا
+
+656
+01:00:01,880 --> 01:00:06,620
+الهوى زيادى جوا بطلع من ال .. بزيد زيادى اتنين
+
+657
+01:00:06,620 --> 01:00:12,160
+الضغط احنا ضغطنا مية و عشرين مش هيك مية و عشرين
+
+658
+01:00:12,160 --> 01:00:17,060
+مظبوط هيك الضغط العادي لما يزيد اتنين على البناية
+
+659
+01:00:17,060 --> 01:00:21,040
+.. مش كتير عشان هيك و انت قاعد جنب زميلتك تسمعيش
+
+660
+01:00:21,040 --> 01:00:26,060
+صوت نفسها الا لا سمح الله كانت مريضة مرض فى صدرها
+
+661
+01:00:26,060 --> 01:00:30,840
+فى مرض فى الجهاز التنفسى بتسمعىتسمعوا طبعا الأزمة
+
+662
+01:00:30,840 --> 01:00:34,140
+بيبقى يسفر تسفير و بدا واحد عنده ارتهاب في الصدر
+
+663
+01:00:34,140 --> 01:00:39,920
+بيبقى يحشرش حجرة ف بيزيد اتنين او بيخس اتنين
+
+664
+01:00:39,920 --> 01:00:52,720
+فبيطلع اللي هو ايش الهواء من صدر الإنسان الآن
+
+665
+01:00:52,720 --> 01:01:06,980
+عشان نعمل احنا تنفس صناعيكيف بنعمل تنفس صناعي؟ هو
+
+666
+01:01:06,980 --> 01:01:16,940
+السؤال الأول قبل هذا لمن يعمل تنفس صناعي؟ مين
+
+667
+01:01:16,940 --> 01:01:21,260
+بأيدها عاد مغم عليها؟ مين عمره شافت حد مغم عليه؟
+
+668
+01:01:21,260 --> 01:01:27,200
+كتير أنا شوف حد مغم عليه و بتنفس الغرجان
+
+669
+01:01:27,200 --> 01:01:28,820
+مين عمره غرجت؟
+
+670
+01:01:31,320 --> 01:01:34,340
+أنا غرجت خاطرا و طلعوا مني ماعملوش تنفس صناعي
+
+671
+01:01:34,340 --> 01:01:39,220
+طلعوا منك ميه؟ نعم و لا بطلعوا ميه، لحق لا بتطلع
+
+672
+01:01:39,220 --> 01:01:44,080
+غرجان و بتنفس، غرج نتف صغير و طلعنا، بدهوا تنفس
+
+673
+01:01:44,080 --> 01:01:48,440
+صناعي ليه؟ احنا قاعدين بنا نعمل لمين تنفس صناعي؟
+
+674
+01:01:48,440 --> 01:01:55,180
+المرض؟ اللي معاه أزمة يعني عمرك شوفتي واحد عنده
+
+675
+01:01:55,180 --> 01:01:58,900
+أزمة بيعملولي تنفس صناعي؟ كان لجهة الشارع مدى
+
+676
+01:01:58,900 --> 01:02:04,080
+الناس بيعملوا تنفس صناعي، لأنه ..بالظبط بنعمل تنفس
+
+677
+01:02:04,080 --> 01:02:10,200
+صناعي لوحد توقف عنده التنفس واحد توقف عنده التنفس
+
+678
+01:02:10,200 --> 01:02:13,120
+ومش كل الناس اللي توقف عندهم التنفس بعملهم تنفس
+
+679
+01:02:13,120 --> 01:02:18,780
+صناعي ولكلهم حتى
+
+680
+01:02:18,780 --> 01:02:25,440
+لو مارجعش مش كل الناس طبعا يعني واحد عنده مريضة او
+
+681
+01:02:25,440 --> 01:02:31,220
+مريض اللي بينازع ست شهورو اتوقف التنفس عنده بعمله
+
+682
+01:02:31,220 --> 01:02:39,680
+تنفس صناعي؟ اه؟ فانت تعالى اسألى كيف حماتك .. من
+
+683
+01:02:39,680 --> 01:02:45,060
+هنا نقول سؤال يعني ما تزعلوش منه نقولك كيف حماتك؟
+
+684
+01:02:45,060 --> 01:02:47,740
+كنت قولت يا سيخ الله يتغمدها بروحته ايش يعني الله
+
+685
+01:02:47,740 --> 01:02:52,240
+يتغمدها بروحته؟ يعني الله ياخدها مش هيك بالظبط
+
+686
+01:02:52,240 --> 01:02:57,440
+معناتها يعني تعبانة ومتعب اللي حواليها مش هيك؟
+
+687
+01:02:57,440 --> 01:03:01,490
+واحد قيلها ست شهور بتموتفي حالات زي هي اليوم مع
+
+688
+01:03:01,490 --> 01:03:08,310
+التقدم العلمى بيظلوا يرجعوا في البني آدم بيظلوا
+
+689
+01:03:08,310 --> 01:03:12,270
+الواحد يترجع لبد طويل جدا يعنى زمان بقى يوم يومين
+
+690
+01:03:12,270 --> 01:03:17,490
+تلات انطرت و لا رحمة الله اليوم عنده بيبقى الجلب
+
+691
+01:03:17,490 --> 01:03:22,070
+تعبان و بيشتغل ابعطوه اشي الكلا ضارب و بيعطوه اشي
+
+692
+01:03:22,070 --> 01:03:27,990
+الكبد خربان و باخدين بالك و كيفو عنده جلطة في المخ
+
+693
+01:03:27,990 --> 01:03:37,870
+و عنده و عنده و عنده و عنده و عنده و عنده و عنده
+
+694
+01:03:37,870 --> 01:03:39,190
+و عنده و عنده و عنده و عنده و عنده و عنده و عنده و
+
+695
+01:03:39,190 --> 01:03:39,390
+عنده و عنده و عنده و عنده و عنده و عنده و عنده و
+
+696
+01:03:39,390 --> 01:03:39,590
+عنده و عنده و عنده و عنده و عنده و عنده و عنده و
+
+697
+01:03:39,590 --> 01:03:42,010
+عنده و عنده و عنده و عنده و عنده و عنده و عنده و
+
+698
+01:03:42,010 --> 01:03:43,630
+عنده و عنده و عنده و عنده و عنده و عنده و عنده و
+
+699
+01:03:43,630 --> 01:03:50,390
+عنده و عنده و عنده و عنده و عنده
+
+700
+01:03:50,390 --> 01:03:57,800
+و عنده و عنده و عنده وجا دي نمنوكل أنا ويا على صح
+
+701
+01:03:57,800 --> 01:04:02,680
+نواعد، توقف التنفس معاه، في كامل نشاطه، أنا بشتغل
+
+702
+01:04:02,680 --> 01:04:09,020
+السعاف أولي، مظبوط؟ بعمله تنفس صناعي، واحد في أمل
+
+703
+01:04:09,020 --> 01:04:16,780
+كبير من أن أعمله تنفس صناعي، يعني انا يوم .. طب
+
+704
+01:04:16,780 --> 01:04:19,700
+واحد بنازله عشر أيام و لا خمساشار يوم، ان انا
+
+705
+01:04:19,700 --> 01:04:23,820
+أعمله تنفس صناعي؟ انا إيه لمدة طويلة جدا بقابل
+
+706
+01:04:23,820 --> 01:04:28,430
+مستشفياتيعني يمكن ماعملناش تنفس الصناعي في حوالي
+
+707
+01:04:28,430 --> 01:04:34,330
+اربعين سنة مااخدين بالكوا كيف لعشر مااخدين بالكوا
+
+708
+01:04:34,330 --> 01:04:39,030
+كيف و كلهم حالات طارقة يعني انا عملت تنفس الصناعي
+
+709
+01:04:39,030 --> 01:04:44,890
+لوحد كان في السوق جاي يتسوق في وسط السوق و جاء
+
+710
+01:04:44,890 --> 01:04:48,010
+حملوه جابوه ع المستشفى عملتوا تنفس الصناعي مثلا
+
+711
+01:04:48,010 --> 01:04:55,480
+جارتنا امروح من الدوامجاعد ع باب البيت ع باب دارهم
+
+712
+01:04:55,480 --> 01:04:59,680
+بالظبط يعني ده الباب مفتوح مرة كبيرة شوية في السن
+
+713
+01:04:59,680 --> 01:05:02,960
+نصها برا الدار و نصها جوه الدار مش هيك الأجازي
+
+714
+01:05:02,960 --> 01:05:09,620
+بيعملوها زيك بتعمل وماشي قدرا يعني يصدقوا هذا
+
+715
+01:05:09,620 --> 01:05:14,960
+الشارع ما بأمر منه إلا نادرا فمريت من هذا الشارع
+
+716
+01:05:14,960 --> 01:05:19,440
+بتطلع في جارتنا اللي جارتنا بتزرج و فجدت الواقع
+
+717
+01:05:19,440 --> 01:05:23,860
+فخشيت جراي عملت لتنفس صناعة طابتربنا يحفظ سبعانه
+
+718
+01:05:23,860 --> 01:05:27,160
+وتعالى سهل و طابت هي اللي بعملها و انا قعدت مدة
+
+719
+01:05:27,160 --> 01:05:34,040
+طويلة جدا بعدها و هي عاشة لكن واحد يعني حماتي اللي
+
+720
+01:05:34,040 --> 01:05:41,640
+بيجي حوالي عشر ايام بتنازع و انا قاعد و الكل مشدود
+
+721
+01:05:41,640 --> 01:05:44,860
+حواليها يعني و الكل هذا شوية قلتلهم عظم الله
+
+722
+01:05:44,860 --> 01:05:50,200
+اجركوا طبعا انا قاعد و بنتها دكتورة طبيبة قاعدين
+
+723
+01:05:51,310 --> 01:05:54,950
+قلتله اعظم الله جل كل حاجة ماتت فقلتلهم ماتت خلاص
+
+724
+01:05:54,950 --> 01:05:59,670
+واخدين بالك كيف ماعملناش تنفس الصناعي علشان هي
+
+725
+01:05:59,670 --> 01:06:04,710
+لعشر أيام بتموت يعني وكل جاعة بستنى فى اللحظة انه
+
+726
+01:06:04,710 --> 01:06:08,470
+يقولها فى أمان الله لرحمة الله فالتنفس الصناعي
+
+727
+01:06:08,470 --> 01:06:15,150
+بنعمل لواحد ايش فى فايدة من ان اعمله تنفس صناعى
+
+728
+01:06:15,150 --> 01:06:21,710
+وفى أمل انه يستجيب وفى أمل انه يصحىماشي المرة
+
+729
+01:06:21,710 --> 01:06:30,430
+الجاية ان شاء الله و الله مانسانة بحنا هذا ان شاء
+
+730
+01:06:30,430 --> 01:06:34,210
+الله هنحكيه فيما بعد في حد عنده أي سؤال في اللي
+
+731
+01:06:34,210 --> 01:06:40,230
+فات؟ اي سؤال من اللي فات؟
+
+732
+01:06:40,230 --> 01:06:46,170
+كل اللي فات مش بس المحاضرة هذه يعني مش سامع
+
+733
+01:06:49,620 --> 01:06:53,500
+تسمم الحمل يا صبايا سمعتوا في حاجة اسم تسمم حمل؟
+
+734
+01:06:53,500 --> 01:06:59,400
+في حاجة اسمها تسمم حمل هذه التسمم الحمل غالبا
+
+735
+01:06:59,400 --> 01:07:05,580
+بيصير مع الصبايا البُكر إيش عن البُكر؟ الأول غالبا
+
+736
+01:07:05,580 --> 01:07:12,140
+بس قد يحدث مع اللي مش بُكر كمان كأنه الجسم بيعرفش
+
+737
+01:07:12,140 --> 01:07:18,970
+يستقبل الجنين جديد بيحسه يعني إش تانيفببدأ الجسم
+
+738
+01:07:18,970 --> 01:07:23,550
+يعني بده .. بده يتعامل معاه بطريقة غلط فبصير عند
+
+739
+01:07:23,550 --> 01:07:31,390
+المرأة تلت حاجات تلت حاجات الحاجة الأولى ورم بصير
+
+740
+01:07:31,390 --> 01:07:33,710
+الورم في الإيدين وفي الرجلين وفي الوجه وفي كل
+
+741
+01:07:33,710 --> 01:07:44,010
+الجسم تلت الحاجة التانية بصير ارتفاع ضغط ارتفاع
+
+742
+01:07:44,010 --> 01:07:47,960
+الضغط بعمل الصداع والزغلل واللخنة هذهبصير ارتفاع
+
+743
+01:07:47,960 --> 01:07:54,760
+ضغط الحاجة التالتة لو فحصنا ball بطلع فيه زولال
+
+744
+01:07:54,760 --> 01:08:02,080
+بطلع في ال ball زولال زولال protein ال protein
+
+745
+01:08:02,080 --> 01:08:08,340
+بكون ال proteinها بنزل في ال ball ماشي فبتتنفخ
+
+746
+01:08:08,340 --> 01:08:15,160
+ضغطها برتفعبولا بيسير فيه زولالة طبعا هذه الست
+
+747
+01:08:15,160 --> 01:08:21,100
+الأصل أنها تتعالج و تتحمى الأصل تتعالج و تتحمى في
+
+748
+01:08:21,100 --> 01:08:25,380
+علاج و في تحمية وذا وما بيداش تتعالج و تتحمى هي
+
+749
+01:08:25,380 --> 01:08:29,760
+حرة يعني أنا قاعد بقول انت سمعتش قاعد بقول في
+
+750
+01:08:29,760 --> 01:08:35,980
+الآخر إذا بيداش تتعالج و تتحمى هي حرة يعني في حدا
+
+751
+01:08:35,980 --> 01:08:42,820
+بيخصني بكريه و عندها زولالةو عندها .. اللي بيقول
+
+752
+01:08:42,820 --> 01:08:50,140
+.. الضغط عالي و عندها تورم يعني بنسميها إيش؟ اللي
+
+753
+01:08:50,140 --> 01:09:02,180
+هي تسمم حمل .. سميها تسمم حمل بتفخر
+
+754
+01:09:02,180 --> 01:09:07,260
+.. بتتنفخ .. عادي .. جديش أنت الحين بتصير جدك مرة
+
+755
+01:09:07,260 --> 01:09:10,180
+و مصر أو مرتين وجهك بيكبر و ديك بيكبر
+
+756
+01:09:13,270 --> 01:09:16,490
+لأ بس مش لهذا الدرجة يعني مش لهذا الدرجة و ممكن في
+
+757
+01:09:16,490 --> 01:09:20,610
+الشهر التامن او التاسع يصير حاجة بسيطة فهذه الست
+
+758
+01:09:20,610 --> 01:09:26,790
+يا بركر يهديك ربنا يا بنت الحلال اتحمي ماتتحميش
+
+759
+01:09:26,790 --> 01:09:33,890
+المهم حملة بتوع مولاد و أبدا ركبت مخها و مابدهاش
+
+760
+01:09:33,890 --> 01:09:39,290
+فبكلمها و بتقول ما هي أصلا بنت خالتي .. بنت خالتها
+
+761
+01:09:39,290 --> 01:09:42,840
+عاديبتعرفش ولا حاجة في دنياتها وبتاخد معلوماتها من
+
+762
+01:09:42,840 --> 01:09:46,560
+بين خالتها المهم إيش اللي صار فيها استوأت يوم
+
+763
+01:09:46,560 --> 01:09:50,020
+نيمنها في المستشفى، نامت في المستشفى، فروحت أزورها
+
+764
+01:09:50,020 --> 01:09:54,380
+في المستشفى، اموص يجرتها تجيب ملح معاها من الدار
+
+765
+01:09:54,380 --> 01:10:00,100
+تصوروا؟ عشان بيجيبولها الأكل، دي لأ ممنوع هي تاخد
+
+766
+01:10:00,100 --> 01:10:04,520
+أملاح، ممنوعة لأن الملح بزيد الضغط و بزيد التنفخ
+
+767
+01:10:04,520 --> 01:10:11,410
+وفعلا عملولها قيصريةومات الولد الأول ومات الولد
+
+768
+01:10:11,410 --> 01:10:16,970
+التاني و بينهم أبوه أسبوع زمان من سيادتها وهي
+
+769
+01:10:16,970 --> 01:10:22,410
+لليوم تعمل قيصرية ماهي للأسف جامعية بخمس جامعة
+
+770
+01:10:22,410 --> 01:10:27,450
+ماخدين بالكوا كيف؟ فيعني الجثة أنه مش عارف الباب
+
+771
+01:10:27,450 --> 01:10:32,450
+الصحيح اللي تدجه و تاخد معلومات من مين فبتقولك بنت
+
+772
+01:10:32,450 --> 01:10:36,110
+خالتي و بنت خالي و بنت عمتيبنت خالتها و بنت خالة و
+
+773
+01:10:36,110 --> 01:10:39,810
+بنت عامتها يعني إيش .. إيش بتعرف ال .. فسألوا أهل
+
+774
+01:10:39,810 --> 01:10:43,270
+الذكري إن كنتم لأ تعلموا .. اللي واحد بدور على
+
+775
+01:10:43,270 --> 01:10:46,610
+اللي باعرف و الله و يعني اللي اليوم مسكينة يبقى
+
+776
+01:10:46,610 --> 01:10:52,830
+عامل قيصرية ووضعها يعني مش سهل فتسمم الحمل اللي هو
+
+777
+01:10:52,830 --> 01:10:57,690
+هذه الشغلات إذا الواحد انتبهله و اتعالج بمشي كويس
+
+778
+01:10:57,690 --> 01:11:02,530
+و غالبا البطن اللي جاي يكون فش اه ان شاء الله مين
+
+779
+01:11:02,530 --> 01:11:03,470
+هي السؤال عندنا؟
+
diff --git a/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/ToF1ruxgrGo_postprocess.srt b/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/ToF1ruxgrGo_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..b5303eb0562c0d21deb7c20a18286c80c98625b3
--- /dev/null
+++ b/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/ToF1ruxgrGo_postprocess.srt
@@ -0,0 +1,3252 @@
+1
+00:00:20,970 --> 00:00:25,290
+بسم الله الرحمن الرحيم ان شاء الله اليوم هنبدأ في
+
+2
+00:00:25,290 --> 00:00:33,550
+الحروق هناخد تعريف الحروق أسباب الحروق أنواع
+
+3
+00:00:33,550 --> 00:00:39,510
+الحروق مساحة الحروق الإسعاف الأولي للحروق وكمان
+
+4
+00:00:39,510 --> 00:00:44,670
+هنحكي عن مخاطر الحروق ماشي الحال لو قلنا إيش
+
+5
+00:00:44,670 --> 00:00:49,870
+الحرق؟ مين تعرفلنا الحرق؟ ما هو الحرق؟
+
+6
+00:00:53,390 --> 00:01:04,490
+طلب يصيب خلايا وأنسدة الجسم تتعرض لمواد بتتغير
+
+7
+00:01:04,490 --> 00:01:10,950
+من طبيعتها تتغير من طبيعة هذه الخلايا وبتغير
+
+8
+00:01:10,950 --> 00:01:16,250
+وظيفتها وبالتالي بيصير air عملها مش العمل الطبيعي
+
+9
+00:01:16,250 --> 00:01:24,280
+لو جينا مثلا ضربنا مثل كيف الحرارةبتغير طبيعة شيء،
+
+10
+00:01:24,280 --> 00:01:30,080
+في حاجة أتصور أنه يعني يوميا بتتعاملوا معاها،
+
+11
+00:01:30,080 --> 00:01:35,380
+النهار بتغير طبيعة حاجات، زي إيش عندكوا في البيت؟
+
+12
+00:01:35,380 --> 00:01:44,160
+إيش بيغير؟ إيش
+
+13
+00:01:44,160 --> 00:01:47,920
+بيصير في الأكل؟ إيش تغير في طبيعته؟
+
+14
+00:01:55,070 --> 00:02:03,470
+طب مين .. مين أفطرت اليوم؟ إيش أفطرت؟ أنت
+
+15
+00:02:03,470 --> 00:02:07,630
+إيش أفطرت؟
+
+16
+00:02:07,630 --> 00:02:17,280
+إيش أفطرت؟ فول؟ ماشي لو جينا قولنا جداش البيضةبدها
+
+17
+00:02:17,280 --> 00:02:22,580
+عشان تفجس اذا وضعت تحت الجاجة وكانت طبعا بيضة
+
+18
+00:02:22,580 --> 00:02:28,420
+ملققه ملققه بده واحد و عشرين يوم ماشي نحط بيضة
+
+19
+00:02:28,420 --> 00:02:36,120
+واحد و عشرين يوم تحت جاجة جاهزة لأنها يعني ترجد
+
+20
+00:02:36,120 --> 00:02:43,460
+واحد و عشرين يوم بتفجس لو هذه البيضة نفسها سلجناها
+
+21
+00:02:44,660 --> 00:02:48,520
+وحطناها تحت الجاجة اكاميون بدها عشان تفجس تطلع
+
+22
+00:02:48,520 --> 00:02:53,100
+منها صوص طب
+
+23
+00:02:53,100 --> 00:03:00,000
+مانفعش؟ مانفعش؟ طب ليش مانفعش؟ طب ماشي ليش؟ انتوا
+
+24
+00:03:00,000 --> 00:03:04,420
+ملاحظين ان انتوا مش مربطين في المادة المادة الحروق
+
+25
+00:03:04,420 --> 00:03:09,640
+والتعريف بيقول تلف يصيب خلايا وأنسجة الجسم نتيجة
+
+26
+00:03:09,640 --> 00:03:16,330
+تعرضها لمواد تغير من طبيعتهاتغيرت طبيعتها فافقدتها
+
+27
+00:03:16,330 --> 00:03:22,710
+خواصها وضفها كان خواص البيض هذى انه تفجس و تطلع
+
+28
+00:03:22,710 --> 00:03:28,650
+صوص بعد 21 يوم اذا تعرضت لجو المناسب لما تعرضت
+
+29
+00:03:28,650 --> 00:03:35,610
+للحرارة حطيها 21 أسبوع مش يوم بتعفن و بتخرج ايش
+
+30
+00:03:35,610 --> 00:03:43,050
+اللي اتغير الحرارة افقدتها خواصها و ضائفها منها
+
+31
+00:03:43,740 --> 00:03:49,940
+بطلت تصير بيضا تفجس صوص و لا لأ و الفول لو جيبنا
+
+32
+00:03:49,940 --> 00:03:55,980
+حبة فول حبة فول و نجعناها و حطناها مثلا على جطمة
+
+33
+00:03:55,980 --> 00:04:00,760
+بعد أكم من يوم بتصير إيش بتلعلها شرش و بتلعلها ورق
+
+34
+00:04:00,760 --> 00:04:05,340
+عمركوا وجعت منكوا حبة حمص حبة فول هادية في المطبخ
+
+35
+00:04:05,340 --> 00:04:13,630
+أه كنا نعملها إيش كانت تصير تطلع حبة البطاطاتطلع
+
+36
+00:04:13,630 --> 00:04:23,670
+لو سلجناها طب إيش اللي صار؟ تغيرت طبيعتها و فقدت
+
+37
+00:04:23,670 --> 00:04:27,770
+خواصها و ضائف حملها كذلك الحروق في جسم الإنسان
+
+38
+00:04:27,770 --> 00:04:34,150
+بتغير من طبيعة الجسم و بتفقد هذا الجسم إيش؟ خواصه
+
+39
+00:04:34,150 --> 00:04:38,970
+يعني بيصير الشكل مش هو خواص الجلد مثلا أنه يدي شكل
+
+40
+00:04:38,970 --> 00:04:44,040
+معينخواص الجسم يدى إحساس معين، خواص الجلد يدى
+
+41
+00:04:44,040 --> 00:04:48,420
+للجسم يعمل عمل معين، الحروق إذا اتعرض إلى هذا
+
+42
+00:04:48,420 --> 00:04:54,080
+الجسم، بيفقد هذا الخواص، ولا لأ؟ مبنقن عليه يعني،
+
+43
+00:04:54,080 --> 00:04:59,840
+هيك بنعرف إيش معنات فقد الخواص و الوضع نوجينا نقول
+
+44
+00:04:59,840 --> 00:05:02,980
+يعني إيش اللي بيخلي الواحد ينحرج، في حاجات كتير
+
+45
+00:05:02,980 --> 00:05:08,300
+بتخلي الواحد ينحرجمين عارف .. مين عارف كده حرارة
+
+46
+00:05:08,300 --> 00:05:14,700
+النار في الدنيا؟ هذا جهنم،
+
+47
+00:05:14,700 --> 00:05:19,160
+نار جهنم، جزء من .. من سبعين جزء من نار جهنم، طب
+
+48
+00:05:19,160 --> 00:05:25,820
+كده نارنا؟
+
+49
+00:05:25,820 --> 00:05:30,580
+النار اللي بنولعها هذه، الغاز و النار و ال .. كده؟
+
+50
+00:05:30,580 --> 00:05:38,360
+إيش خمس و عشرين؟ آلافألاف ألاف مش تحت الصفر ألاف
+
+51
+00:05:38,360 --> 00:05:45,560
+ماشي فالنار الحرارة العالية هذه لو أجت على الجسم
+
+52
+00:05:45,560 --> 00:05:53,080
+ممكن تعمل تلاف و ممكن ماتعملش ولا شي ولاش رايكوا
+
+53
+00:05:53,080 --> 00:05:58,760
+مامشكلتش حاطيت يدك على النار بسويش اشي اتحسنت يعني
+
+54
+00:05:58,760 --> 00:06:06,470
+مش هيك مش هيك اتحسنتبمعنى انه بدنا حرارة عالية
+
+55
+00:06:06,470 --> 00:06:14,130
+عشان يصير حرق كمان بدنا زمن بدنا زمن يكفي لأنه
+
+56
+00:06:14,130 --> 00:06:19,230
+يقدى لحدوث الحرق الآن ربنا سبحانه وتعالى ماذا يقول
+
+57
+00:06:19,230 --> 00:06:25,490
+في كتابه العزيز احنا اتفجنا انه نار الدنيا جزء من
+
+58
+00:06:25,490 --> 00:06:30,530
+سبعين جزء من نار جهنم جزء من سبعين جزء من نار جهنم
+
+59
+00:06:30,530 --> 00:06:35,030
+طب احنا قاعدةأبواب رمضان كل عبد و انتوا بخير ان
+
+60
+00:06:35,030 --> 00:06:38,930
+شاء الله و ربنا يبلغنا رمضان و يعيننا على صيام و
+
+61
+00:06:38,930 --> 00:06:44,490
+قيامه عشان ان شاء الله مانروحش عن النار فالنار هذه
+
+62
+00:06:44,490 --> 00:06:49,450
+جزء من سبعين جزء ربنا سبحانه وتعالى قال عن نار
+
+63
+00:06:49,450 --> 00:06:56,670
+جهنم اللي جد نار الدنيا بسبعين ضعف اللي ربنا أوقل
+
+64
+00:06:56,670 --> 00:07:04,640
+عليها الف سنةحتى سودت حتى بيضت و ألف سنة حتى حمرت
+
+65
+00:07:04,640 --> 00:07:09,400
+و ألف سنة حتى سودت فهي الآن سوداء قاتمة تلتلاف سنة
+
+66
+00:07:09,400 --> 00:07:15,640
+ماشي هذه النار وإن منكم إلا واردوها مش أنا بقول
+
+67
+00:07:15,640 --> 00:07:23,040
+الله اللي بقول صح؟ ايش يعني؟ كل الناس لازم يوردوها
+
+68
+00:07:23,040 --> 00:07:31,050
+مش يشوفوها لازم يمروا عنها لازم يمروا عنهاالمرور
+
+69
+00:07:31,050 --> 00:07:38,590
+عنها يعني كنا بننحرج، النبي بيان إيش قال؟ قال إن
+
+70
+00:07:38,590 --> 00:07:43,950
+الناس على قدر أعمالهم، والله اللي بيعمل ممتاز،
+
+71
+00:07:43,950 --> 00:07:49,850
+بيمر زي البرق اللامع، برج يعني مافيش وجد، الوجد
+
+72
+00:07:49,850 --> 00:07:56,170
+صفر، اللي أجل شوية أبطأ شويةالأقل وأقل أبطأ أكتر
+
+73
+00:07:56,170 --> 00:08:02,810
+فناس بت .. بتت جرح ناس بتت حرج أطرافها ناس ناس ناس
+
+74
+00:08:02,810 --> 00:08:07,690
+وناس بتجعى فوالله يكوننا مين يعني ناس يقول الله
+
+75
+00:08:07,690 --> 00:08:12,150
+العفو والعافية فهذا النار الناس اللي بخشه منها
+
+76
+00:08:12,150 --> 00:08:17,810
+الناس الصالحين الأدقياء بخشه زي البرجفهدولا لا
+
+77
+00:08:17,810 --> 00:08:21,330
+يشعروا بال .. بتسير النار كما قال الرسول صلى الله
+
+78
+00:08:21,330 --> 00:08:27,330
+عليه و سلم يا مسلم أسرع فقد أوشك نورك أن يطفئ ناري
+
+79
+00:08:27,330 --> 00:08:30,850
+يا سلام اللهم اجعلنا منهم يا رب .. قولوا امين
+
+80
+00:08:30,850 --> 00:08:37,570
+فبمعنى انه مافيش زمن عشان يكون في حرج بدي انا زمن
+
+81
+00:08:37,570 --> 00:08:43,650
+ارتفاع درجة حرارة بدي زمن فبدي نار و بدي زمن و بدي
+
+82
+00:08:43,650 --> 00:08:48,150
+جسم ينحرجالحرارة العالى هذه من وين ممكن تيجي من
+
+83
+00:08:48,150 --> 00:08:52,830
+النار الجافة النار الجافة اللى كلكوا بتولعوها نار
+
+84
+00:08:52,830 --> 00:08:59,750
+الغاز، نار الحطب، نار البابور،
+
+85
+00:08:59,750 --> 00:09:05,150
+نار الورق، أي نار بنولعها بتعمل ما يسمى بالنار
+
+86
+00:09:05,150 --> 00:09:14,110
+الجافة، نار الكهربا، الخبز لو بنخبزه على الخبز
+
+87
+00:09:14,110 --> 00:09:19,750
+الكهربا، مش نارعشان يستوي بده نار الفرن الكهربا
+
+88
+00:09:19,750 --> 00:09:24,230
+برضه اللى بنشوفى و بنعمل برضه بنعمل النار فالنار
+
+89
+00:09:24,230 --> 00:09:30,930
+او اتباعها النار او اتباعها ممكن سبب حرق ايش يعني
+
+90
+00:09:30,930 --> 00:09:39,610
+اتباعها اتباع النار اتباع النار جنب النار الجمر
+
+91
+00:09:39,610 --> 00:09:46,250
+بيضلوا نار فانحنا احنا استخدمنا النارعشان نطبخ
+
+92
+00:09:46,250 --> 00:09:52,690
+طبيخ طبيخ و استخدمنا المعلقة أو الملعقة للحركة
+
+93
+00:09:52,690 --> 00:09:57,890
+الحركة .. النار موصلتش للمعلقة لكن المعلقة بتصير
+
+94
+00:09:57,890 --> 00:10:01,890
+سخن زي النار نفس
+
+95
+00:10:01,890 --> 00:10:07,830
+الغاز بصير سخن بتقدريش تحط إيديكي على المجلة عشان
+
+96
+00:10:07,830 --> 00:10:13,530
+أنت مسكين إيد البراد الشايبتقدريش تمسكيه لأن هذه
+
+97
+00:10:13,530 --> 00:10:19,770
+كلها أتباع نار و ممكن تحريك و لا بتحريكش فهذه تسمى
+
+98
+00:10:19,770 --> 00:10:25,970
+النار و أتباعها الحاجة التانية اللي ممكن تكون هي
+
+99
+00:10:25,970 --> 00:10:33,870
+الغاز الساخن الغاز الساخن من وين بيجي؟ عمرك خبزتي؟
+
+100
+00:10:35,160 --> 00:10:42,100
+و نفس لرغيف على إيدك شواركش شاطر على عقب بابجي
+
+101
+00:10:42,100 --> 00:10:46,740
+الواحد يخبز و بتنخزج نطفة من الرغيف و بتطلع دخنة
+
+102
+00:10:46,740 --> 00:10:54,860
+هوا غاز سخن كأنه نار بيحرج طرف الأصابع حصلت ولا لأ
+
+103
+00:10:54,860 --> 00:10:59,820
+كل كو شفتوها بتهيألي كتير بالظبط بتحصل كتير هذا
+
+104
+00:10:59,820 --> 00:11:04,680
+غاز سخن الغاز الساخن اللي بيطلع من تناجر الضغط
+
+105
+00:11:05,870 --> 00:11:11,650
+طنجرة الضغط بتطلع منها ايش؟ غاز ساخن جدا عارفين
+
+106
+00:11:11,650 --> 00:11:16,930
+انه بتطلع ساخن جدا جدا حوالي تلاتمية وخمسين درجة
+
+107
+00:11:16,930 --> 00:11:21,350
+او اكتر فيهين انه البخار اللى بتطلع من الطنجرة
+
+108
+00:11:21,350 --> 00:11:28,110
+العادية مية فالغاز السخن ممكن جدا يعمل ما يسمى
+
+109
+00:11:28,110 --> 00:11:29,370
+بالحروق
+
+110
+00:11:31,990 --> 00:11:38,410
+طبعا الغازات السخنة هذه إذا استنشقتيها تعمل مصيبة
+
+111
+00:11:38,410 --> 00:11:43,130
+ممكن تعملك تسكير للجيوب الأنفية و تسكر الرئتين و
+
+112
+00:11:43,130 --> 00:11:46,950
+تلتهب الرئتين و تخنق الواحد و موت كتير من الناس
+
+113
+00:11:46,950 --> 00:11:51,870
+بيموتوا نتيجة استنشاق ايش أو بيصير عندهم مشاكل
+
+114
+00:11:51,870 --> 00:11:57,170
+نتيجة استنشاق الغازات الساخنة الحاجة التالت
+
+115
+00:11:57,170 --> 00:12:04,320
+الحرارة سواء السخنةسواء للسخنة، تهيألة سواء للسخنة
+
+116
+00:12:04,320 --> 00:12:13,880
+يعني ده بالبلد، الشاي، مرقة الطعام، كل ما بنسخنه
+
+117
+00:12:13,880 --> 00:12:20,980
+المي اللى بنستخدم فيها المي السخنة، حرقتى بمي
+
+118
+00:12:20,980 --> 00:12:27,800
+سخنة؟ كيه؟ جولىو كان مجرد مدعوم في الجراسة كان
+
+119
+00:12:27,800 --> 00:12:34,640
+حاسب فرق و متعدد بقى جد على طولة نعم فمحكيلي ..
+
+120
+00:12:34,640 --> 00:12:37,780
+الشيانين محكيلي طولة محكيلي بقى تجمعي عطالة و ماني
+
+121
+00:12:37,780 --> 00:12:43,600
+عطالة اشتغلت الطولة بالتجارة؟ ولا تجمعك اتعرضى؟
+
+122
+00:12:43,600 --> 00:12:50,340
+تجمعك اتعرضى على اسم الدولة؟ على اسم الدولة؟ نعم ف
+
+123
+00:12:50,340 --> 00:12:50,600
+..
+
+124
+00:12:53,350 --> 00:12:57,710
+ان شاء الله يعني الحمد لله طبتي، الحمد لله، الحمد
+
+125
+00:12:57,710 --> 00:13:03,830
+لله رب العالمين، فالسوائل الساخنة، المي المغلية،
+
+126
+00:13:03,830 --> 00:13:10,310
+المرجة الطبيخ، الشاي، كل هذه عبارة عن إيش؟ سوائل
+
+127
+00:13:10,310 --> 00:13:15,510
+ساخنة، من الحاجات كمان اللي بتأدي إلى الحروق،
+
+128
+00:13:15,510 --> 00:13:20,230
+المواد الكيميائية، مثلا ميت النار، أنا بعرف شاب
+
+129
+00:13:20,230 --> 00:13:27,570
+شرب ميت نار بالخطأحرجت الجهاز الغضبي، البلعوم سكر
+
+130
+00:13:27,570 --> 00:13:33,950
+و لزج في بعضه فكانوا حاطوله باربيش من الفم او من
+
+131
+00:13:33,950 --> 00:13:40,830
+الأنف و بوكل عن طريق البربيش طبعا اش هي .. ما ..
+
+132
+00:13:40,830 --> 00:13:44,490
+اش هيسوله فيما بعد؟ هيجيبوا البلعوم و يحطوله جزء
+
+133
+00:13:44,490 --> 00:13:50,700
+من الأمعاء عشان تفتح الطريق، سكرةمصورة المجرة
+
+134
+00:13:50,700 --> 00:13:55,540
+الجهاز الهضمى سكرت فبنغيّر المصورة يا بنفتحها يا
+
+135
+00:13:55,540 --> 00:13:59,940
+بن .. إيش؟ حينما شريان في القلب بيسكر شو بنعمله؟
+
+136
+00:13:59,940 --> 00:14:03,540
+بنجيبه و بنحط واحد بداله لإن لو استقصلنا و جيبنا
+
+137
+00:14:03,540 --> 00:14:07,920
+ما هو مسكر مافيش مشكلة خلّي زي ما هو لكن كونه
+
+138
+00:14:07,920 --> 00:14:13,200
+بيغذي جزء معين أو إله وظيفة لا يستغنى عنها بنحط
+
+139
+00:14:13,200 --> 00:14:18,000
+بداله فممكن جدا الأشياء الكيميائية زي مويت الله
+
+140
+00:14:19,250 --> 00:14:24,950
+المركزة زي ال chlorine زي الصودة الكاوية كثير
+
+141
+00:14:24,950 --> 00:14:27,950
+الدار مدانة حاجات زهيرة، اليوم الله أرحم ستة يوم
+
+142
+00:14:27,950 --> 00:14:34,610
+كان الزمان تقعد جنب هالرمل و تجيب شوية كاز و تحطه
+
+143
+00:14:34,610 --> 00:14:38,590
+على الرمل و تجلد جلي سيريال معلمة ويه المعلمة؟ على
+
+144
+00:14:38,590 --> 00:14:44,280
+هنا مابظبطش معانا هالحكي، ليش مابظبطش؟وين بدنا نحط
+
+145
+00:14:44,280 --> 00:14:47,760
+الرمل اللى بدنا نطلعها؟ مافيش مكان نحط فيه الرمل
+
+146
+00:14:47,760 --> 00:14:51,740
+هيسكروا المجاري ويسكروا الدار وبعدين اليوم فى
+
+147
+00:14:51,740 --> 00:14:56,580
+مصاري الحمد لله فى فلوس ونقل عليها يعني فى لكن هذا
+
+148
+00:14:56,580 --> 00:15:01,800
+عمل خطورة أخرى اللى صار فى مواد كيمائية موجودة
+
+149
+00:15:01,800 --> 00:15:05,940
+اللى هى عوامل ضرر موجودة فى البيت ممكن الأطفال
+
+150
+00:15:05,940 --> 00:15:12,330
+يشربوها ممكن أنت تشربيها تنحرجى تيجي عليكىكل هذه
+
+151
+00:15:12,330 --> 00:15:22,750
+الشغلات بتعمل ما يسمى الحرق الكيميائي القلويات
+
+152
+00:15:22,750 --> 00:15:25,310
+المشتقاتة، من الحاجات اللي كمان ممكن تعمل حرق
+
+153
+00:15:25,310 --> 00:15:31,170
+الكهربا، الكهربا بتحرق، ذاكرين حكينا عن الكهربا؟
+
+154
+00:15:31,170 --> 00:15:36,330
+إيش قلنا؟ كيف الكهربا بتحرق؟ مين ذاكرة؟
+
+155
+00:15:40,270 --> 00:15:44,330
+هو كيف الكهرة بيبتحرق؟
+
+156
+00:15:44,330 --> 00:15:48,810
+الكهرة بيبتحرق أنه بيجي عندنا فيشة فيشة حاجها
+
+157
+00:15:48,810 --> 00:15:53,790
+شكلين أو شكلين نص بنشبصها بنحط عليها لزاج و
+
+158
+00:15:53,790 --> 00:15:57,430
+بنتعجها و كده فبيجي واحد بيمسكها اللي هي عاملة أو
+
+159
+00:15:57,430 --> 00:16:01,450
+اللي هي مولعة الدنيا عاملة قصر أو عاملة شورت و
+
+160
+00:16:01,450 --> 00:16:05,690
+مولعة يعني في واحد من جيراننا قبل كام من يوم اللي
+
+161
+00:16:05,690 --> 00:16:09,210
+دارهم مولعة إيش القصة؟ عنده مشترك هذا بالتلت عيون
+
+162
+00:16:09,920 --> 00:16:14,040
+حطوا جنب الخزانة و طالعين برا فصار المشترك فيه
+
+163
+00:16:14,040 --> 00:16:18,120
+خلال و ضالوا يلمع يلمع يلمع و يعمل شرار لما ولع ال
+
+164
+00:16:18,120 --> 00:16:21,500
+plastic الخارجية ال plastic الخارجية مربوطة ..
+
+165
+00:16:21,500 --> 00:16:24,440
+مربوطة جنب الخزانة و الخزانة جنب التخت إلا الدار
+
+166
+00:16:24,440 --> 00:16:29,920
+مولعة إلا الدار مولعة عشان ما نوفر خمس شكل أو أربع
+
+167
+00:16:29,920 --> 00:16:33,520
+شكل بندفع خمسين ألف دنار لو كان مصريهم كلهم هناك
+
+168
+00:16:33,520 --> 00:16:37,580
+مثلا بنحرقهم قولت لأ و الدار بتبقى محرقة و تلعب
+
+169
+00:16:38,630 --> 00:16:44,270
+ممكن جدا الكهربا تسببنا ما يسبب الحروق كمان قولنا
+
+170
+00:16:44,270 --> 00:16:49,930
+ان الكهربا بتعمل تأيين في الجسم و بتعمل حروق ..
+
+171
+00:16:49,930 --> 00:16:54,870
+بتعمل حروق مش بسيطة انا شوفت واحد من أصحابي كان
+
+172
+00:16:54,870 --> 00:17:01,790
+جنبي مسكته الكهربا إصبعه هذا انعوج وظل اليوم زاوية
+
+173
+00:17:01,790 --> 00:17:08,410
+قائمة صدقوني أمين منك من جباليابتعرف واحد اسمه خضر
+
+174
+00:17:08,410 --> 00:17:16,630
+محجز تسمعه في .. بيشتغل في .. تطلع فيه أصبعه واحد
+
+175
+00:17:16,630 --> 00:17:20,990
+من أصابعه هيتلاقيه يعود هذا انصاب الكهربا وان هو
+
+176
+00:17:20,990 --> 00:17:27,290
+واقف جنبه من الكهربا صار حرق وانتنى الأصبع ويعني
+
+177
+00:17:27,290 --> 00:17:31,070
+طبعا هو مبسوط جدا ومن الأصبع مراحش بقى اسمه موجود
+
+178
+00:17:31,070 --> 00:17:37,860
+مش هي فالحروق بتسببها الكهرباكمان قولنالكوا انه
+
+179
+00:17:37,860 --> 00:17:43,780
+شفنا البرج ذاكرات و قولتلكوا انه مكان دخول الكهربا
+
+180
+00:17:43,780 --> 00:17:48,660
+و مكان خروج الكهربا بسبب ايش بسبب حروق .. حروق
+
+181
+00:17:48,660 --> 00:17:54,680
+سيئة من الحاجات اللي بتعمل اللي هي الإشعاعات بتعمل
+
+182
+00:17:54,680 --> 00:17:59,540
+حروق و الورانيوم ذاكرين أيام ما ضربوا اليهود ضربوا
+
+183
+00:17:59,540 --> 00:18:06,010
+البلد بالورانيوم كان بيعمل حرق .. نعمالفسوار
+
+184
+00:18:06,010 --> 00:18:10,630
+واليراني والمنظب هذا .. الفسوارة فال .. ال ..
+
+185
+00:18:10,630 --> 00:18:14,890
+الإشعاعات اللى انضربت فيها هورشيما ومجزاكا سمعتوا
+
+186
+00:18:14,890 --> 00:18:19,890
+عنها؟ اه؟ امريكا اللى قاعدة بتقولك حقوق الإنسان،
+
+187
+00:18:19,890 --> 00:18:25,050
+ضربوا البلد بال .. ال .. لحد اليوم الناس بيلدهم
+
+188
+00:18:25,050 --> 00:18:31,440
+مشوهين و الأرض بتنبتشعشرات السنين صار و الأرض لسه
+
+189
+00:18:31,440 --> 00:18:36,500
+أثار المواد الإشاعية و المذر الذرات و الكذا بتعمل
+
+190
+00:18:36,500 --> 00:18:40,080
+مشاكل في البني أدمين و بتعمل مشاكل في النباتات و
+
+191
+00:18:40,080 --> 00:18:44,620
+بتعمل مشاكل في كل المخلوقات ماخدين بالكوا كيف؟
+
+192
+00:18:44,620 --> 00:18:48,240
+فالإشعاعات الذرية هذه و الإشعاعات اللي بيستخدموها
+
+193
+00:18:48,240 --> 00:18:52,540
+في الحروب و الإشعاعات اللي بيستخدموها لعلاج مرض
+
+194
+00:18:52,540 --> 00:18:58,560
+السلطان مش مرض السلطان بيستخدموه شعاععشان إشعاع
+
+195
+00:18:58,560 --> 00:19:04,400
+كيميائي هذا بيخلي المرض السرطاني موت بس برضه بيأثر
+
+196
+00:19:04,400 --> 00:19:08,720
+على المرض على الخلايا الحيه العادية وما أخدين بالك
+
+197
+00:19:08,720 --> 00:19:12,640
+وكيف عشان هيك يعني اللي باخد هذا بتتعامل مع
+
+198
+00:19:12,640 --> 00:19:18,840
+إفرازاته بطريقة خاصة جدا يعني مخاته عرقه وكذا لأنه
+
+199
+00:19:18,840 --> 00:19:22,960
+ممكن ينضج الإشعاع كمان من هنا بني أدم قدر فبناء
+
+200
+00:19:22,960 --> 00:19:26,960
+عليه الإشعاعات ممكن تعمل مشاكل
+
+201
+00:19:29,150 --> 00:19:40,110
+واضح الكلام؟ خوف اه؟ واضح الكلام؟ نعم الحروف
+
+202
+00:19:40,110 --> 00:19:46,150
+يا أخوات المجدات يعني لا تقف عند درجة الحروف فقط
+
+203
+00:19:46,150 --> 00:19:53,050
+أو عند مكان الحرج فقط ماخدين بالكم؟ فإذا المخاطر
+
+204
+00:19:53,050 --> 00:19:57,070
+الأضافية للحرج أنه بيصير استنشاق غازات سامة
+
+205
+00:19:57,070 --> 00:20:01,720
+واختناقاتإذا كان حد فيكوا أجى أظهر الجامعة أيام من
+
+206
+00:20:01,720 --> 00:20:08,360
+حرقات كل أو معظم الحرق في الدور الأول صح؟ مافيش
+
+207
+00:20:08,360 --> 00:20:13,700
+دور تاني محروق مين بتقول ليش؟ طيب مين بتحلل ليش؟
+
+208
+00:20:13,700 --> 00:20:19,740
+لا الله أول حاجة حماها ثانيا اللي أجى سيادته حرق،
+
+209
+00:20:19,740 --> 00:20:25,700
+حرق من تحت بدأ يحرق تحت و بعدين طلع فوق فلما حرق
+
+210
+00:20:25,700 --> 00:20:30,410
+تحتصارت الدخن و الحم و الكذا و بدى يطلع فوق لحجة
+
+211
+00:20:30,410 --> 00:20:37,350
+الدخن خنجته فشرد تصورين انتوا كيف؟ خنجته الدخن
+
+212
+00:20:37,350 --> 00:20:43,670
+فشرد فمن المخاطر الضافية كيف؟ ما كلهم بيشتغلوا من
+
+213
+00:20:43,670 --> 00:20:49,030
+تحت بقولك كل اللي بده بحرج و كان بده من تحت ربنا
+
+214
+00:20:49,030 --> 00:20:52,140
+سبحانه أن الله يدافع على الذين آمنوافربنا بدوا
+
+215
+00:20:52,140 --> 00:20:57,420
+يحمل هذا بدوا يشتغلوا من تحت بدت الدخنة تطلع تعمل
+
+216
+00:20:57,420 --> 00:21:00,940
+اختناقات وهذه استنشاق الغازات الصادرة عن المواد
+
+217
+00:21:00,940 --> 00:21:07,920
+المحترقة تعمل اختناقات لما يسير حرق في أي مكان إذا
+
+218
+00:21:07,920 --> 00:21:11,720
+بتتذكروا أبراج اللي ضربوهم الشباب في أمريكا
+
+219
+00:21:11,720 --> 00:21:14,780
+الأبراج
+
+220
+00:21:14,780 --> 00:21:17,140
+التجارة .. أه
+
+221
+00:21:19,880 --> 00:21:26,140
+هذه الأبراج إذا بتذكروا وشوفتوا الصور كانت النار
+
+222
+00:21:26,140 --> 00:21:31,320
+تمشي فيها أسرع ما تمشي النار في الورق يعني لو جبت
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+223
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+كالتون وحطتيها على الغاز بتاخد وقت أكتر من منهال
+
+224
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+البرج لاحظوها؟ فسبحان الله العظيم أمريكا بعتت على
+
+225
+00:21:42,240 --> 00:21:49,680
+القمر سفينة سمتها التحديchallenger يعني المتحدة،
+
+226
+00:21:49,680 --> 00:21:56,120
+يعني مين بتتحدى مين؟ مجرد .. هاذي challenger بعد
+
+227
+00:21:56,120 --> 00:22:00,340
+دقيقة و نص من قدوعها، هى و اللى فيها راحت، بتتحدى
+
+228
+00:22:00,340 --> 00:22:06,610
+مين انت؟ف ال .. الناس هدول الأمريكان بدهم يعني
+
+229
+00:22:06,610 --> 00:22:11,870
+يشتغلوا ب .. بحماوة السدر فأجى حوالي بقولك اتنين و
+
+230
+00:22:11,870 --> 00:22:17,670
+سبعين مدرب DNS مصعف و مطفي .. رجل اطفاء اتنين و
+
+231
+00:22:17,670 --> 00:22:22,710
+سبعين واحد مصعف و رجل اطفاء بدهم يخشوا .. مجرد ما
+
+232
+00:22:22,710 --> 00:22:27,370
+خشوا المبنى كان نازل فوقهم كان نازل المبنى فوقهم
+
+233
+00:22:27,370 --> 00:22:32,300
+فزاد .. زاد العدد اتنين و سبعين انسان متخصصفهذه من
+
+234
+00:22:32,300 --> 00:22:35,340
+الحاجات اللي هي الانهيارات اللي بتحدث نتيجة
+
+235
+00:22:35,340 --> 00:22:40,180
+النيران النار بتعمل انهيارات كمان بتعمل اختنقات
+
+236
+00:22:40,180 --> 00:22:43,880
+ماخدين بالكوا كيف وبناء عليه يعني لما تيجي تتعاملي
+
+237
+00:22:43,880 --> 00:22:48,920
+مع مكان فيه انهيارات او فيه حروق او كذا بدك تهتمي
+
+238
+00:22:48,920 --> 00:22:53,740
+انه انت يعني تفض المكان على السريع لأنه ممكن ينهار
+
+239
+00:22:53,740 --> 00:23:00,300
+او ممكن يختنق منه البني ادمي واضحفي اشي بدنا نعيده
+
+240
+00:23:00,300 --> 00:23:09,320
+عرفتوا ليش البيض بفجسش؟ ليش بفجسش البيض؟ لأنه
+
+241
+00:23:09,320 --> 00:23:14,960
+النار غير الطبيعته و وظيفة عمله و هيك الحريق بساوي
+
+242
+00:23:14,960 --> 00:23:21,960
+في أن الحروق حاجة اسمها درجات الحروق درجات
+
+243
+00:23:21,960 --> 00:23:22,920
+الحروق هذه
+
+244
+00:23:26,750 --> 00:23:30,350
+درجة أولى و تانى و تالتى و في ناس بتقول رابع طبعا
+
+245
+00:23:30,350 --> 00:23:34,270
+ايش اللى بيعمل الدرجة للحريق اللى بيعمل الدرجة
+
+246
+00:23:34,270 --> 00:23:41,470
+للحريق كمية الشعلة او كمية المادة الحارقة و المد
+
+247
+00:23:41,470 --> 00:23:47,930
+طول المد كمية الحرارة و نوعية الحرارة و طول المد
+
+248
+00:23:47,930 --> 00:23:54,310
+فيعني مثلا الدرجة الأولىطبعا في درجة أولى و تانية
+
+249
+00:23:54,310 --> 00:23:58,710
+و تالتة و في ناس بيقولوا رابعة الحرج مصفط بالعكس
+
+250
+00:23:58,710 --> 00:24:05,430
+كل ما كان الدرجة أقل كل ما كان أخف يعني مش الأولى
+
+251
+00:24:05,430 --> 00:24:09,830
+في الجامعة هي الأشطر زي الأولى في الحرج هي .. في
+
+252
+00:24:09,830 --> 00:24:14,090
+الحرج هي الأكتر لأ الأولى في الجامعة هي الأشطر
+
+253
+00:24:14,090 --> 00:24:18,590
+الأولى في الحرج هي الأخف هي البسيطة اللى يعني كأنه
+
+254
+00:24:18,590 --> 00:24:22,870
+مافيش حرجماشي؟ إيش ممكن يسببنا حرق من الدرجة
+
+255
+00:24:22,870 --> 00:24:33,290
+الأولى؟ إيش ممكن يسببوا؟ حاجة بسيطة .. حاجة بسيطة
+
+256
+00:24:33,290 --> 00:24:37,890
+من النهار أو من الحرارة أو من المادة الكيميائية
+
+257
+00:24:37,890 --> 00:24:42,730
+تيجي على الجسم لمدة بسيطة أو سياتك بتروحي على
+
+258
+00:24:42,730 --> 00:24:47,370
+البحر تقعدي ست أشهر و أنت تترجى أهلك يا أبا بنروح
+
+259
+00:24:48,820 --> 00:24:52,740
+بقولك يا ابني اتش و كله شباب و وين و انا بغار عليك
+
+260
+00:24:52,740 --> 00:24:56,800
+و ابصرشو فبرضهاش يبعتك فمجرد ماتروح تقعد على البحر
+
+261
+00:24:56,800 --> 00:25:02,620
+بتظلك من الصبح للمغرب و انت ع الشاطر تقعد ..
+
+262
+00:25:02,620 --> 00:25:07,200
+تتروحي من البحر محروقة .. محروقة حارج من الدرج
+
+263
+00:25:07,200 --> 00:25:11,700
+الأول حارج و في ممكن بعض المناطق توصل لدرج تاني من
+
+264
+00:25:11,700 --> 00:25:16,800
+الشمس فحارجالدرجة الأولى عبارة عن نوع بسيط بيصير
+
+265
+00:25:16,800 --> 00:25:21,660
+شوية أحمرار تغيير بسيط في الجلد شوية ورم خفيف مع
+
+266
+00:25:21,660 --> 00:25:28,140
+شوية ألم بسيط هذا ممكن من الميه دافية شوية ممكن من
+
+267
+00:25:28,140 --> 00:25:33,920
+اللي هو الشمس ممكن من نار لمدة قصيرة جدا يعني
+
+268
+00:25:33,920 --> 00:25:38,760
+تعملك حرق من إيش الدرجة الأولى تخدونكوا قصة على
+
+269
+00:25:38,760 --> 00:25:42,650
+هذا الموضوعدى عبادة كنت سمعه في الظهر صلوا عنا
+
+270
+00:25:42,650 --> 00:25:47,630
+فضيف .. ضيفنا نكرمه من مطبخنا مافيش عنه لا كرم ولا
+
+271
+00:25:47,630 --> 00:25:51,690
+.. أول ما ييجي الواحد نعمله شاي مش شاي؟ ولا
+
+272
+00:25:51,690 --> 00:25:56,070
+بتعملوه شاي في دوره؟ اه يعني هذه اللي بدك تكرميه
+
+273
+00:25:56,070 --> 00:26:04,210
+أول ما مقعده بتجيبيله شاي، مظبوط؟ ممكن فقعدنا كان
+
+274
+00:26:04,210 --> 00:26:10,080
+أيامها بيستخدم المبورمافيش غاز فجريبنا قاعد هنا و
+
+275
+00:26:10,080 --> 00:26:13,960
+عنده الغاز و عنده البابورة و الشاي و كذا و أنا
+
+276
+00:26:13,960 --> 00:26:16,940
+قاعد جنب جريبنا و فيه كمان ضيف قاعد مننا و هنا
+
+277
+00:26:16,940 --> 00:26:24,920
+الحين مجرد ما خلصت الشاي صب و جريبنا هذا صاحب
+
+278
+00:26:24,920 --> 00:26:28,800
+المحل مد ايده هنا و الضيف مد ايده هنا و صاروا
+
+279
+00:26:28,800 --> 00:26:35,830
+اتنين فوق رجليه و جعت منهم الكباية عليك صدقونيوجعت
+
+280
+00:26:35,830 --> 00:26:40,250
+الكباية عليا جراي انا قلت بيدي هيك لأ تلقاه يعنى
+
+281
+00:26:40,250 --> 00:26:45,090
+فجزء من الميه الشاي او الشاي السخن هذا اجعللي جنبي
+
+282
+00:26:45,090 --> 00:26:51,310
+اجعللي جنبي جراي فيه حنافية قدامي روحت على الميه
+
+283
+00:26:51,310 --> 00:26:55,750
+اغسل اغسل اغسل قوم يا ابو فلان قالك لأ يا عم مش
+
+284
+00:26:55,750 --> 00:26:59,890
+مستاهل وقولك يا عم قوم أبدا مارضيش احنا قلنا ايش
+
+285
+00:26:59,890 --> 00:27:06,750
+بسبب الحوادث مين ذاكره الجهل الجهلو الإهمال و
+
+286
+00:27:06,750 --> 00:27:12,190
+التحدي واحد بيركى براسه يعنى خلاص بأنده فالزمان
+
+287
+00:27:12,190 --> 00:27:16,390
+اتحدى خلاص يا راجل قال ابدا بفضل الله انا صار عندى
+
+288
+00:27:16,390 --> 00:27:19,190
+حارج من الدرجة الاولى فقط هو صار عنده حارج من
+
+289
+00:27:19,190 --> 00:27:23,610
+الدرجة التانى وضلوا عدة ايام و هو يغير ولا
+
+290
+00:27:23,610 --> 00:27:27,890
+يقولنالك ايه قال اه ايش بالنسبة و لا يسحبوا و يجوم
+
+291
+00:27:27,890 --> 00:27:31,870
+تحدي ايش يقولوا الناس هنا يعنى عشان نتفض مليجة
+
+292
+00:27:31,870 --> 00:27:37,560
+كعجوم قعدت بدك ناس تفهم يعنىفالحرق من الدرجة
+
+293
+00:27:37,560 --> 00:27:43,540
+الأولى لو أجت حاجة ساخنة لمدة قصيرة جدا والحجتيها
+
+294
+00:27:43,540 --> 00:27:48,120
+بالاسعاف السريع ممكن مايصيرش الا حرق من الدرجة
+
+295
+00:27:48,120 --> 00:27:53,720
+الأولى عبارة عن حمرار تغير بسيط في لون الجلد ورم
+
+296
+00:27:53,720 --> 00:28:00,440
+أحساس بألم بسيط وغالبا بطيب الواحد بدون أي تشوهان
+
+297
+00:28:04,930 --> 00:28:09,930
+هو يعني بدناش نخلي معجون لإسنان سبب احنا لازم نغسل
+
+298
+00:28:09,930 --> 00:28:13,330
+أول أنا بحبش أعط معجون لإسنان ليش بحبش أعط مانوعي
+
+299
+00:28:13,330 --> 00:28:17,730
+معجون لإسنان عمرك روحتي تعمل معجون .. إسنانك
+
+300
+00:28:17,730 --> 00:28:23,010
+وانشغلتي وضل المعجون على الفرشاية فترة بترجع
+
+301
+00:28:23,010 --> 00:28:29,360
+بتلاقي زي الحطب مظبوط منشف فلما هذا ينحط على جرحأو
+
+302
+00:28:29,360 --> 00:28:34,080
+على جلد وينشف ممكن يعمل مشاكل نانيج نجيبه في
+
+303
+00:28:34,080 --> 00:28:44,000
+المستشفى ممكن نسلخ فأحسن ميه أحسن أيش؟ ميه مش على
+
+304
+00:28:44,000 --> 00:28:47,440
+كل إشي بنحط هنحك إن شاء الله وقت إيش نحط تلج وقت
+
+305
+00:28:47,440 --> 00:28:55,420
+إيش مانحطش تلج الدرجة التانية الدرجة التانية من
+
+306
+00:28:55,420 --> 00:29:03,840
+الحروق بكونأعمقه من الدرجة الأولى بتكون فقاقية
+
+307
+00:29:03,840 --> 00:29:10,020
+مائية العمق الحرق أكبر من الدرجة الأولى و تشمل
+
+308
+00:29:10,020 --> 00:29:14,160
+الطبقة متعددة الجذران اللي تحت الجل بيصير إحمرار
+
+309
+00:29:14,160 --> 00:29:18,700
+الجلد و بتبدأ تبين الفقاقية المائية أو النتوئات
+
+310
+00:29:18,700 --> 00:29:23,300
+نافخة الإيد و فيها ميه وين بتشوف الإيد نافخة و
+
+311
+00:29:23,300 --> 00:29:30,140
+فيها نتوئات ميهبكون هذا حرق من الدرجة الثانية ماشي
+
+312
+00:29:30,140 --> 00:29:36,240
+ف الحرارة ممكن تكون اكثر او المدة اكثر او التنين
+
+313
+00:29:36,240 --> 00:29:43,100
+معاها و تعمل اللي هو فقاقية مائية الفقاقية
+
+314
+00:29:43,100 --> 00:29:47,760
+المائية دي عبارة عن ايش محتوياتالدم مع ذاكرات الدم
+
+315
+00:29:47,760 --> 00:29:52,260
+الحمرا والبيضا يعني اللي هي البلازمة البلازمة اللي
+
+316
+00:29:52,260 --> 00:29:59,740
+موجودة في الجسم وبتكون سبب يعني تلف الجد في درجة
+
+317
+00:29:59,740 --> 00:30:08,080
+تالتة الدرجة التالتة معناه ذلك انه حرق لمدة أطول
+
+318
+00:30:08,080 --> 00:30:16,330
+وحرارة أعلى حرق لمدة أطول وحرارة أعلىنيران الغماس
+
+319
+00:30:16,330 --> 00:30:21,110
+في ماء ساخن جدا و الكهربا و كلها تعتمد على درجة
+
+320
+00:30:21,110 --> 00:30:25,430
+الحرارة درجة الحرارة اللي هي عالية و مدة التعرض
+
+321
+00:30:25,430 --> 00:30:31,070
+زيادة المدة فبتكون المدة طويلة و درجة الحرارة أكتر
+
+322
+00:30:31,070 --> 00:30:33,510
+و ممكن يكون الحرق كيميائي عمل الحرق من الدرجة
+
+323
+00:30:33,510 --> 00:30:38,210
+التالتة يعني برضه التعرض بكون لمدة أكتر و كمية
+
+324
+00:30:38,210 --> 00:30:44,660
+المادة الكيميائية أكبرطبعا إيش بيصير .. بيصير حرق
+
+325
+00:30:44,660 --> 00:30:50,260
+الأنسجة العميقة طرق الطبقة اللي هي القاعدية بتتأثر
+
+326
+00:30:50,260 --> 00:30:55,760
+و بيصير الجلد في أوله لونه كأنه أبيض لأنه الأوعية
+
+327
+00:30:55,760 --> 00:31:02,780
+الدموية تنشف و مابينش الدم فيها و بتصير في أولها
+
+328
+00:31:02,780 --> 00:31:08,480
+نعم بتصير الطبقة القاعدية بتتأثر و لازم عشان يطيب
+
+329
+00:31:08,480 --> 00:31:15,220
+هذا النوع من الحرقلازم تدخل جراحي و بعدين في حاجة
+
+330
+00:31:15,220 --> 00:31:20,020
+اسمها الدرجة الرابعة درجة حر من الدرجة الرابعة بعض
+
+331
+00:31:20,020 --> 00:31:24,200
+الناس بيقول و بعض الناس بيحط الرابعة و الثالثة مع
+
+332
+00:31:24,200 --> 00:31:29,600
+بعض اللي قالوا الرابعة قالوا إذا صار تفحم في
+
+333
+00:31:29,600 --> 00:31:34,300
+المكان تسمى درجة رابعة فبطلع مين أختار الدرجات
+
+334
+00:31:38,320 --> 00:31:45,420
+ماشي قولنا قبل شوية كيف خطورة الحرق؟ بتتحدد بإيش؟
+
+335
+00:31:45,420 --> 00:31:51,340
+بالمدة وكمية الحرارة بالمدة وكمية الحرارة و المكان
+
+336
+00:31:51,340 --> 00:31:57,140
+و المكان الآن واحد عنده حرق درجة رابعة على أصبع
+
+337
+00:31:57,140 --> 00:32:03,380
+وماسكنه ورا منها بدنا شيها اشي المشكلة كتير واحد
+
+338
+00:32:03,380 --> 00:32:08,230
+عنده درجة حرارة واحد عنده حرق درجة تالتةوماخد جزء
+
+339
+00:32:08,230 --> 00:32:13,290
+كبير من جسمه، بيكون بده موت، بيكون عنده باباة،
+
+340
+00:32:13,290 --> 00:32:17,270
+فمين بيطلع أخطر؟ حسب عالج المصابة، انتوا ملاحظين
+
+341
+00:32:17,270 --> 00:32:23,350
+احنا قاعدين يعني بن .. بنهتم بإنه انت خدي معلومات
+
+342
+00:32:23,350 --> 00:32:28,410
+كاملة عن اللي جمالك، يعني ايه ايه تسمعي بس درجة
+
+343
+00:32:28,410 --> 00:32:35,990
+حرارة رابعية بقى؟اه هذا طعن نافذ .. و الكافر دي
+
+344
+00:32:35,990 --> 00:32:41,110
+جبالة؟ كافر مصاب؟ ولا لأ؟ و بناء عليه بتحدد
+
+345
+00:32:41,110 --> 00:32:45,790
+الخطورة دكتور في ناس بنحرج و بتضل السنين؟ بتضل
+
+346
+00:32:45,790 --> 00:32:52,010
+العمر اه احنا قلنا انه الحرج او الالتئام بالقصد
+
+347
+00:32:52,010 --> 00:32:57,970
+التانيبترك تشوهات و ممكن يترك مثلا أنه مايقدرش
+
+348
+00:32:57,970 --> 00:33:01,610
+يحركيهم، مايقدرش يحرك وجهم الجلد تتم كل فترة و
+
+349
+00:33:01,610 --> 00:33:05,590
+فترة، ليش ما يتغير الجلد؟ الجلد الخارجي خالص، لكن
+
+350
+00:33:05,590 --> 00:33:12,610
+هذا صار مش جلد، صار .. صار هذا تليف، صار تليف، صار
+
+351
+00:33:12,610 --> 00:33:17,710
+نمو بالقصد التاني، مش هيك؟ حكينا ارجع قول الاجر
+
+352
+00:33:17,710 --> 00:33:20,610
+القصد الأول والقصد التاني، هتشوفي إيش الفرق
+
+353
+00:33:24,880 --> 00:33:29,780
+الان هذا الصورة عندكم في الكتاب صح؟ الان احنا
+
+354
+00:33:29,780 --> 00:33:43,300
+بنيجي نقول حرق 10% 7% 50% 13% 18% ايش يعني حرق 18%
+
+355
+00:33:43,300 --> 00:33:54,110
+15% 10% طبعا هذا بيسموه مساحة الحرقمقداش من الجسم
+
+356
+00:33:54,110 --> 00:34:01,870
+انحرج بياخد نفسه قسموا طبعا الكبار او البالغين الى
+
+357
+00:34:01,870 --> 00:34:10,510
+نظام سموه نظام التسعة سموه
+
+358
+00:34:10,510 --> 00:34:20,130
+نظام التسعة كيف هذا عملوه نظام
+
+359
+00:34:20,130 --> 00:34:20,650
+التسعة
+
+360
+00:34:23,370 --> 00:34:26,310
+بدي أعطيكوا مفتاح لمعرفة نظام التسعة ده لأن هذا
+
+361
+00:34:26,310 --> 00:34:34,070
+مهم تعرفوه نظام التسعة جلك الإيد الذراع كامل تسعة
+
+362
+00:34:34,070 --> 00:34:39,690
+الذراع تسعة
+
+363
+00:34:39,690 --> 00:34:44,670
+في المية ايش يعني تسعة في المية يعني حجم إيدك
+
+364
+00:34:44,670 --> 00:34:50,390
+كاملة جد تسعة في المية من جسمك طبعا الجسم بالشكل
+
+365
+00:34:50,390 --> 00:34:58,770
+الواضح الطبيعيحوالي 1.6 متر مربع مش كل البشر في
+
+366
+00:34:58,770 --> 00:35:02,650
+ناس يعني انا انا بارح شوفت مره يمكن فيها تلاتة متر
+
+367
+00:35:02,650 --> 00:35:09,350
+مربع و بتشوفي واحد يمكن متر مربع لكن بالمتوسط
+
+368
+00:35:09,350 --> 00:35:15,890
+متوسط العام واحد وست من عشرة متر مربع هذا جسم
+
+369
+00:35:15,890 --> 00:35:23,360
+الإنسان حجم الإنسان جالك الذراعمن البني آدم تسعة
+
+370
+00:35:23,360 --> 00:35:29,800
+فالمية الحين احنا اننا اكمل راقة اتنين فصارت
+
+371
+00:35:29,800 --> 00:35:37,300
+تسعتين ماشي بنانيجي ناخد هذا مفتاح او هذا زي ما
+
+372
+00:35:37,300 --> 00:35:45,120
+بقوله standard ماشي معيار لو قلنا كم جلبابك بيجي
+
+373
+00:35:45,120 --> 00:35:52,790
+على جد رجل بنطلونك يبقىصغير بالنسباله يبقى الساق
+
+374
+00:35:52,790 --> 00:35:59,490
+كاملة الإجار تسعة اتين تسعة
+
+375
+00:35:59,490 --> 00:36:06,410
+تسعة وكم ساق عندي يبقى بطلع تسعة تسعة وضعت في
+
+376
+00:36:06,410 --> 00:36:11,950
+كيفار نجيبها سهل هيك طيب يا ستي برضه بنرجع على
+
+377
+00:36:11,950 --> 00:36:17,490
+المعيار كم الجلباب بيغطي السدر والبطن
+
+378
+00:36:21,450 --> 00:36:27,330
+مجيش هي فك كمك و حطيه مكفيش صح ولا لأ؟ يبقى السدر
+
+379
+00:36:27,330 --> 00:36:34,210
+و البطن تتسعتين صح؟
+
+380
+00:36:34,210 --> 00:36:39,670
+يعني شغل مفهوم ضمنه و مبدأش فلسفة و شطارة الدهر جد
+
+381
+00:36:39,670 --> 00:36:45,830
+السدر و البطن ولا لأ؟ الدهر جد السدر و البطن
+
+382
+00:36:51,370 --> 00:37:00,610
+بدون معدل هيطلع عن 11 هدوها لأ عشرة عشرة مين بيقول
+
+383
+00:37:00,610 --> 00:37:06,410
+ماجيبناش سيرة حاجة الرأس و الرقم الرأس من الأمام و
+
+384
+00:37:06,410 --> 00:37:15,210
+الخلف و الرقم ده تساوي تسعة هيصار أكم تسعة أحداشر
+
+385
+00:37:15,210 --> 00:37:20,510
+تسعة في تسعة تسعة و تسعين طب البناء أدم ذاته قداش
+
+386
+00:37:20,830 --> 00:37:23,910
+مية في المية فبظل واحد في المية اللي هي منطقة
+
+387
+00:37:23,910 --> 00:37:30,890
+العانى منطقة العانى بيسموها واحد في المية هيك صار
+
+388
+00:37:30,890 --> 00:37:38,010
+المجموع مية في المية واضح؟ في اي سؤال في الموضوع؟
+
+389
+00:37:38,010 --> 00:37:42,410
+طبعا هاي انتوا عرفتوا كيف بنجراها لو طلعتي هان
+
+390
+00:37:42,410 --> 00:37:50,420
+بكتبلك في الكتاب ع الساق مثلا تسعةو كاتب ع الشجة
+
+391
+00:37:50,420 --> 00:37:53,000
+التاني تسعة يعني من الأمام تسعة و من الخلف تسعة
+
+392
+00:37:53,000 --> 00:37:58,520
+فبطلع الساق كله جديش تسعتين لكن على الذراع كاتب
+
+393
+00:37:58,520 --> 00:38:02,860
+تسعة و الذراع التاني ولا شيء يبقى الذراع .. الذراع
+
+394
+00:38:02,860 --> 00:38:11,240
+قدام و ورا تسعة واحدة باضح؟ نعم ايش انا بهمني
+
+395
+00:38:11,240 --> 00:38:18,140
+كمسعف ان اعرف تسعة و اتنين و خمسة و خمستاشر18% و
+
+396
+00:38:18,140 --> 00:38:22,920
+50% ايش بهمني بهمني
+
+397
+00:38:22,920 --> 00:38:31,680
+احنا قولنا خطورة الحرق في ايش مساحته و عمقه و
+
+398
+00:38:31,680 --> 00:38:42,460
+مكانه مساحته و عمقه و مكانه فبقولك انه من 1 ل 10%
+
+399
+00:38:42,460 --> 00:38:49,640
+في البالغين ايش مكانة الدرجةمبالزه مش يخش مستشفى
+
+400
+00:38:49,640 --> 00:38:58,920
+فى البلوك ماشى من واحد لعشرة فالمية ايش ماكانت حتى
+
+401
+00:38:58,920 --> 00:39:03,360
+لو درجة تالتة ممكن يتعالج فى العيادة وياخد مضاد
+
+402
+00:39:03,360 --> 00:39:08,780
+حيوى مثلا وغيرات وانتباه مفش داعلى انه ينام ايش
+
+403
+00:39:08,780 --> 00:39:14,160
+المستشفى وبعدين لما نطيب الحرق بده عملية تجميل
+
+404
+00:39:14,160 --> 00:39:27,520
+عملية حاجب نعملها كعملية جراحيةماشي من تلاتين
+
+405
+00:39:27,520 --> 00:39:34,100
+فما فوق هذا ضروري ينام المستشفى ضروري ينام
+
+406
+00:39:34,100 --> 00:39:42,420
+المستشفى خمسين فالمية فما فوق يشكل خطر على حياة
+
+407
+00:39:42,420 --> 00:39:51,350
+البني آدم واحد محروق خمسين فالميةفما فوق في احتمال
+
+408
+00:39:51,350 --> 00:40:02,690
+كبير جدا ان يموت ليش طب بده يموت؟ فما
+
+409
+00:40:02,690 --> 00:40:11,910
+فوق نص جسمه محروب طب إيش المشكلة؟
+
+410
+00:40:11,910 --> 00:40:15,870
+متحرك يعني بجلبه رجليني كل شي أنا اشتغلت مع أكتر
+
+411
+00:40:15,870 --> 00:40:20,960
+من واحداليوم ناكل .. ناكل جايبها اللحمة و كباب و
+
+412
+00:40:20,960 --> 00:40:25,380
+طابق بيتغدى و نتخرف ويا تمام تمام و شبق و شرب عصير
+
+413
+00:40:25,380 --> 00:40:32,100
+و بنروح بكرا بنيجي باللقيمات مانت عينا حرق ايش
+
+414
+00:40:32,100 --> 00:40:35,640
+اللي بصير بالظبط معاهم؟ أمركوا زورت واحد محروق في
+
+415
+00:40:35,640 --> 00:40:43,300
+قسم الحروق ولا في الدار؟ بتروحى على البني آدم روحت
+
+416
+00:40:43,300 --> 00:40:49,630
+زورته؟ قداش كان الحرق نسبة؟وين مكانه أولا؟ في
+
+417
+00:40:49,630 --> 00:40:57,750
+الوجه و الرجبه كيف مخدته دايما؟ صفرة صفرة صح؟ لا
+
+418
+00:40:57,750 --> 00:41:03,210
+سوائل سوائل الجسم فتصوري أن واحد صدره مثلا و دهره
+
+419
+00:41:03,210 --> 00:41:10,070
+أكتر من 50% من جسمه محروق بيكون الجلد تالف فاقد
+
+420
+00:41:10,070 --> 00:41:13,830
+خصائصه من خصائص الجلد أنه بيحتفظ بالسوائل في
+
+421
+00:41:13,830 --> 00:41:20,670
+الداخل فبيصير شر مينالمي هذه معها أملاح جسمنا بدون
+
+422
+00:41:20,670 --> 00:41:27,610
+الأملاح ماعيشش فبظل يفقد أملاح و أهم إشي يعني أنه
+
+423
+00:41:27,610 --> 00:41:35,630
+نوازن الأملاح و نوازن السوائل كمان جثة؟ لأ
+
+424
+00:41:35,630 --> 00:41:40,230
+جثة بتخور فعلا؟
+
+425
+00:41:40,230 --> 00:41:46,010
+مالكيش جثة؟ بلاش هتقول جثة ولا بلاش؟ مين بده جثة؟
+
+426
+00:41:48,870 --> 00:41:56,030
+طيب هي قصة يعني بسيطة في إنا جار هذا الجار يعني
+
+427
+00:41:56,030 --> 00:42:03,770
+أرزق زي ما بقوله عامل من كلمة أرزق فالراجل بظمن
+
+428
+00:42:03,770 --> 00:42:10,870
+زيتون و بوصر الزيتون بعمل زيت و ببيع فهذاك اليوم
+
+429
+00:42:10,870 --> 00:42:19,220
+حاططله هيك سد فوق الغرفةبطون يعني و اعمل عريشة ع
+
+430
+00:42:19,220 --> 00:42:27,140
+الداير اب نخل نخل ناشف بتعمله عريشة بالنخل زي اللى
+
+431
+00:42:27,140 --> 00:42:33,720
+ع البحر فعمله قد التنكات زيت حاططن جنبه مسكين
+
+432
+00:42:33,720 --> 00:42:41,040
+ونايم و هو نايم اجى صاروخ فى المنطقة فجر التنكات
+
+433
+00:42:41,040 --> 00:42:47,680
+وولعل وانحرج اللى هو ورق النخلطبعا انحرج هو .. هو
+
+434
+00:42:47,680 --> 00:42:55,940
+نايم فده لما انحرج وده وين ع مستشفى الشفاء نايم يا
+
+435
+00:42:55,940 --> 00:43:00,920
+صاروخ .. اقولك مثلا الطلق اللي بوكتلك بتدريش فيه
+
+436
+00:43:00,920 --> 00:43:05,760
+.. بتسمعيش صوته .. طلق اللي بوكتلك .. بتسمعيش صوته
+
+437
+00:43:05,760 --> 00:43:10,760
+.. ليش بتسمعيش صوته؟ فتشرديش منه .. مكانه وصلك قبل
+
+438
+00:43:10,760 --> 00:43:11,440
+ما تسمعيه
+
+439
+00:43:14,870 --> 00:43:22,030
+اختبار صحي بس صحي محروق صحي حواليه زيت مولق و
+
+440
+00:43:22,030 --> 00:43:27,010
+حلوية ورق الجريد مولق و الدنيا عايمة و فرجته و
+
+441
+00:43:27,010 --> 00:43:32,110
+بطنيته و الغطاء مولق و أوعيه مولق تبقى أخد بالك
+
+442
+00:43:32,110 --> 00:43:35,550
+حمله و راحوا فيه على مستشفى الشفاء طبعا الجريد ما
+
+443
+00:43:35,550 --> 00:43:37,910
+اسمه إلا جارنا يعني سحبت حالي و روحت أزوره في
+
+444
+00:43:37,910 --> 00:43:41,810
+الشفاء إيش يا جماعة أنا المركز قال والله يحولنا
+
+445
+00:43:41,810 --> 00:43:45,780
+على الداخلحوالناه ع الداخل طبعا هو جار دار أبوي
+
+446
+00:43:45,780 --> 00:43:49,280
+بالظبط روحت ع دار أبوي قاللي يا أبا؟ و قولله نعم
+
+447
+00:43:49,280 --> 00:43:54,060
+قاللي جارنا في المستشفى قولله والله يا أبا زورته و
+
+448
+00:43:54,060 --> 00:43:56,680
+مالقيتوش و مودينه ع الداخل قاللي أه بس الناس
+
+449
+00:43:56,680 --> 00:44:00,220
+بييجوا يسلموا و يواسعوا أهله تروحنا و أنت؟ قولله
+
+450
+00:44:00,220 --> 00:44:07,840
+يا أبا روحت أنا و أبوي وين؟ ع جيراننا قعدنا قدران
+
+451
+00:44:07,840 --> 00:44:13,120
+اللي هو في تليفون من عنده من عنده مرافققالوا اشمنى
+
+452
+00:44:13,120 --> 00:44:18,320
+هل انت موجود؟ وخد كلبه هى اللى استاذ احمد ابراهيم
+
+453
+00:44:18,320 --> 00:44:25,180
+ماسكنا طبعا انا عارف ايش القصة يعنى فقديتهم و
+
+454
+00:44:25,180 --> 00:44:29,660
+قولتلهم حافظوا على السوائل و سجون و حاجة زى ايه بس
+
+455
+00:44:29,660 --> 00:44:33,740
+عارف ايش القصة فبعد ما سكر التليفون ماهوش بقول
+
+456
+00:44:33,740 --> 00:44:39,080
+الراجل شايف؟ قوله اه قال هنا بقى يقولوا حرق 90%
+
+457
+00:44:39,080 --> 00:44:46,730
+هناك قالوا 70%مين اللي انبسط؟ أبويا الله يطمنكوش
+
+458
+00:44:46,730 --> 00:44:53,570
+ممتاز طبعا انا ايش ولا كلمة انا ولا كلمة فلما
+
+459
+00:44:53,570 --> 00:44:56,590
+طلعنا بقولوا يا أبا انت عارف انا ايش بشتغل؟ جالي
+
+460
+00:44:56,590 --> 00:45:01,730
+اه يا أبا ليش ماحكيت؟ قوله يا أبا سبعين و تسعين و
+
+461
+00:45:01,730 --> 00:45:07,750
+ستين فوق الخمسين عند بعض جال انا انا اش بعرف نقوله
+
+462
+00:45:07,750 --> 00:45:11,950
+عشان ايه كانت ضلتني ساكت؟و بديش أقولك يعني أنه مش
+
+463
+00:45:11,950 --> 00:45:17,630
+صح فعلا تاني يوم تالت يوم جاب الراجل جثة هامدة إلى
+
+464
+00:45:17,630 --> 00:45:23,550
+رحمة الله ماخدين بالكم كيف؟ فأكثر من 50% بشكل خطر
+
+465
+00:45:23,550 --> 00:45:28,350
+على حياة الإنسان ليش؟ لأن جسمه بفقد السوائل
+
+466
+00:45:28,350 --> 00:45:34,550
+بالأملاح وجسمك بشتغلش فقط على سوائل وأملاحالجلب
+
+467
+00:45:34,550 --> 00:45:40,290
+بده أملاح النبضات بدها أملاح التوصيل بده أملاح
+
+468
+00:45:40,290 --> 00:45:44,350
+الخلايا بتعيش بالأملاح كل شي بعيش بالسوائل
+
+469
+00:45:44,350 --> 00:45:50,310
+والأملاح فبناء عليها يعني احنا بهيك شغلة بنعرف كيف
+
+470
+00:45:50,310 --> 00:45:58,070
+حالة المريض ففوق 50% بكون وضعه صعب طبعا هذا بالوضع
+
+471
+00:45:58,070 --> 00:46:02,980
+الطبيعي لكن في الوضع الغير طبيعيالاطفال مثلا
+
+472
+00:46:02,980 --> 00:46:08,160
+بتأثروا أكتر بالحرج من الصغار حتى الأطفال اللي لهم
+
+473
+00:46:08,160 --> 00:46:14,220
+نسبة حروج لجسمهم غيرنا يعني 10% بدهم مستشفى 10%
+
+474
+00:46:14,220 --> 00:46:18,460
+بدهم مستشفى كمان إيده ورجله و كده و بطنه بتجسموش
+
+475
+00:46:18,460 --> 00:46:21,960
+زي ما احنا بنجسم تسعة تسعة تسعة فيقولهم تجسيمة
+
+476
+00:46:21,960 --> 00:46:27,340
+خاصة بالإيش بالاطفال لإنه مثلا رأس الطفل الكبير
+
+477
+00:46:27,340 --> 00:46:35,190
+لجسمهاه مثلا إجراء برضه بالنسبة لإيديه كبار فيعني
+
+478
+00:46:35,190 --> 00:46:40,750
+فيه تجسيمات خاصة للأطفال فالطفل مثلا ممكن 10%
+
+479
+00:46:40,750 --> 00:46:48,030
+يحتاج لدخول مستشفى الناس المرضى مريض السكري واحد
+
+480
+00:46:48,030 --> 00:46:54,910
+مريض سكري وانحرج ممكن يخش في مصيب واحد عنده مشاكل
+
+481
+00:46:54,910 --> 00:47:01,060
+مثلا واحد عنده مشاكلكبد .. اي مرض معين ممكن يحتاج
+
+482
+00:47:01,060 --> 00:47:07,840
+الى ايش مشاكله دخول مستشفى كمان في مناطق جد ما
+
+483
+00:47:07,840 --> 00:47:13,940
+يكون الحرج لازم تحتاج مستشفى جد ما يكون الحرج احنا
+
+484
+00:47:13,940 --> 00:47:20,360
+اتفاجنا انه الايد كلها جديش 9% 9% طب بتطلع الكف
+
+485
+00:47:20,360 --> 00:47:26,960
+جديش جليل جداإذا كان في حرج في الكفات، بدأ أدخل
+
+486
+00:47:26,960 --> 00:47:36,040
+مستشفى، قبل .. صح طبعا، ليش؟ هو
+
+487
+00:47:36,040 --> 00:47:39,700
+سبحان
+
+488
+00:47:39,700 --> 00:47:42,820
+الله كل إشي في جسم الإنسان مفيد، حتى الزائد
+
+489
+00:47:42,820 --> 00:47:47,040
+الددية، تصور أن حتى الزائد الددية إلا فايد في جسم
+
+490
+00:47:47,040 --> 00:47:53,480
+الإنسان؟ نعمبتحتفظ بنوع من الميكروبات المفيدة
+
+491
+00:47:53,480 --> 00:47:59,280
+للجسم يعني مفيدة
+
+492
+00:47:59,280 --> 00:48:04,680
+.. اه اللوازم مفيدة للجسم فكل اشي يعني في جسم
+
+493
+00:48:04,680 --> 00:48:13,200
+الإنسان مفيد الآن الجنت جريب و العظم امسك هيك
+
+494
+00:48:13,200 --> 00:48:19,560
+بتلاقي العظم انت ماسك اعظم جديش بين العظمةو الخارج
+
+495
+00:48:19,560 --> 00:48:25,220
+صفة بسيطة جدا فممكن جدا الحرق يوجه الأصابع تلاقي
+
+496
+00:48:25,220 --> 00:48:29,680
+واحد محروق يديه رايحات تكين خالص فإذا هذا بحتاج
+
+497
+00:48:29,680 --> 00:48:36,900
+مستشفى و أصابع القدر فبدنا
+
+498
+00:48:36,900 --> 00:48:38,680
+نروح و نودع المستشفى
+
+499
+00:48:43,420 --> 00:48:49,200
+اه هو الحرق لو ضلوا مكشف مكشف ممكن يلتقم أسرع مش
+
+500
+00:48:49,200 --> 00:48:56,240
+بس الحرق حتى الجروب ماشي فإذا أطراف الأصابع سواء
+
+501
+00:48:56,240 --> 00:48:59,680
+الأيدين أو الرجلين جداش كانت النسبة يحتاج تاخد
+
+502
+00:48:59,680 --> 00:49:06,680
+مستشفى الحاجة التانية منطقة التنفس الوجه و الرجمة
+
+503
+00:49:06,680 --> 00:49:12,850
+واحد بستنشق غازاستنشغ في المستشفى جهازه التنفسى
+
+504
+00:49:12,850 --> 00:49:18,450
+ايش معناه تنحرج؟ معناه تورم عمرك و مين انحرجت
+
+505
+00:49:18,450 --> 00:49:24,910
+عمرها؟ هي كلنا انحرجنا ايش بيصير بعد الحرج؟ ايش
+
+506
+00:49:24,910 --> 00:49:30,470
+بيصير بعدها؟ بيصير ورا شبتران؟ تصوري انت جهاز
+
+507
+00:49:30,470 --> 00:49:37,230
+التنفسى استنشغتى غاز سخن او انحرجتى حيرم ايش هيصير
+
+508
+00:49:37,230 --> 00:49:42,150
+في جهاز التنفسى؟ بسكربختنق البهر فإيش بنساوله في
+
+509
+00:49:42,150 --> 00:49:49,490
+المستشفى هذا مجرد ما يصل بيحطوله انبوب تنفس انبوب
+
+510
+00:49:49,490 --> 00:49:54,170
+تنفس في الحنجرة وفي القصب الهوائية انبوب التنفس
+
+511
+00:49:54,170 --> 00:50:01,630
+هذا قد ما يرم من حين كيف الكهرة بيواصل عنها في في
+
+512
+00:50:01,630 --> 00:50:08,430
+وسط البطون بربيش وفي في وسط البربيش سلوكفجد ما
+
+513
+00:50:08,430 --> 00:50:12,170
+تحطي بطون بيظل البربيش فاته فالبربيش هذا او
+
+514
+00:50:12,170 --> 00:50:17,810
+الأنبوب هذا اللى بنحطه فى الحنجرة حتى لو ورن بيظل
+
+515
+00:50:17,810 --> 00:50:24,850
+ايش فاته طريق التنفس و لا لأ فإذا منطقة التنفس هذه
+
+516
+00:50:24,850 --> 00:50:28,670
+بتحتاج دخول مستشفى كمان منطقة العينا كلها واحد
+
+517
+00:50:28,670 --> 00:50:34,530
+فالمية بتحتاج دخول مستشفى ليش؟ من جسم الحساب كل
+
+518
+00:50:34,530 --> 00:50:41,650
+جسم الإنسان حسابنعم؟ منطقة العنا تحتاج تدخل مستشفى
+
+519
+00:50:41,650 --> 00:50:52,410
+ليه؟ خطر أو حساسة ليه؟ هو كل الجلد الطفل أو الصغير
+
+520
+00:50:52,410 --> 00:51:00,310
+أو الكبير كمان شوية بده يبول لو ما بده يبول ما
+
+521
+00:51:00,310 --> 00:51:06,130
+قلنا الحرق بتربي، سكّر مجرد البولسكر مجلة البول
+
+522
+00:51:06,130 --> 00:51:10,970
+اذا ما بولش تنفجر المكانة وبتخرب الجهاز ال كده
+
+523
+00:51:10,970 --> 00:51:14,790
+الجهاز البولي بخرب فمجرد ما يصل واحد عنده حرق
+
+524
+00:51:14,790 --> 00:51:21,130
+بيحطله قسطرة انبوب بيصير يطلع البول برا ماظبطش
+
+525
+00:51:21,130 --> 00:51:25,930
+القسطرة بنحطله فتحة تحت الصرع بنحط بربيج وبصير
+
+526
+00:51:25,930 --> 00:51:32,390
+البول يطلع حتى يتم الشفاء والالتئامفبصيرش انفجار و
+
+527
+00:51:32,390 --> 00:51:36,330
+بصيرش تسمم و بصيرش مشاكل اللى ممكن تنتج و يلغوا
+
+528
+00:51:36,330 --> 00:51:41,730
+منه الحرق لا يتجاوز واحد فالمية فإذا المنطقة و
+
+529
+00:51:41,730 --> 00:51:47,380
+حالة المكانالها دور في انه يدخل مستشفى او مايدخلش
+
+530
+00:51:47,380 --> 00:51:50,640
+مستشفى احنا بتعرف ان الحارج يعني كتير اللي بتقاسيه
+
+531
+00:51:50,640 --> 00:51:52,780
+بس ماعرفش انه هالجامعين اللي هم اللي هم اللي هم
+
+532
+00:51:52,780 --> 00:51:54,700
+اللي هم اللي هم اللي هم اللي هم اللي هم اللي هم
+
+533
+00:51:54,700 --> 00:51:58,060
+اللي هم اللي هم اللي هم اللي هم اللي هم اللي هم
+
+534
+00:51:58,060 --> 00:51:59,780
+اللي هم اللي هم اللي هم اللي هم اللي هم اللي هم
+
+535
+00:51:59,780 --> 00:51:59,820
+اللي هم اللي هم اللي هم اللي هم اللي هم اللي هم
+
+536
+00:51:59,820 --> 00:52:05,660
+اللي
+
+537
+00:52:05,660 --> 00:52:09,200
+هم
+
+538
+00:52:20,470 --> 00:52:25,790
+الثاني والله أحيانا بيضل و أحيانا لا، في التجميل
+
+539
+00:52:25,790 --> 00:52:32,210
+يعني بتتظبط، بتتظبط، بتتظبط تصبيط يعني، أنت في
+
+540
+00:52:32,210 --> 00:52:37,170
+التجميل إيش بتساوي؟ إيد بتتحركش، بتحاول تعمليلها
+
+541
+00:52:37,170 --> 00:52:44,690
+.. تجيمل إش تليفأو الخلايا الناشرة منطقة مثلا
+
+542
+00:52:44,690 --> 00:52:50,310
+شكلها مش مقبول أيه بس اللي بنعمله في التجميل منطقة
+
+543
+00:52:50,310 --> 00:52:55,110
+مش راضية الطيب خالص بنعمل رجعة مش هيك؟ تسمعوا
+
+544
+00:52:55,110 --> 00:52:59,610
+بيعملوا رجعة؟ إيش يعني رجعة؟ بنجيب من الفخد مثلا
+
+545
+00:52:59,610 --> 00:53:04,930
+أو من مكان أو بنحط جلد عشان تساعد على الشفاف احنا
+
+546
+00:53:04,930 --> 00:53:09,800
+قلنا مساحة سطح البني آدم واحد وستة من عشرةعن كتبلك
+
+547
+00:53:09,800 --> 00:53:16,200
+16 ومش ومش 16 وعبارة عن جسمناها إلى نظام التسعة
+
+548
+00:53:16,200 --> 00:53:23,740
+وهذه جنة بتختلف في من؟ في الأطفال الآن كيف بدنا
+
+549
+00:53:23,740 --> 00:53:31,000
+نسعف حالات الحروب مش بس حالات الحروب كل حالة بدنا
+
+550
+00:53:31,000 --> 00:53:37,720
+نسعفها أول حاجة بنعملها بنبعد المصاب عن منطقة
+
+551
+00:53:37,720 --> 00:53:44,170
+الخطرأو نبعد منطقة الخطر عن المصاب دايما بقدر أبعد
+
+552
+00:53:44,170 --> 00:53:48,130
+المصاب عن منطقة الخطر بس أحيانا بقدر أبعد الخطر عن
+
+553
+00:53:48,130 --> 00:53:54,050
+الإصابة يعني واحد مسكال كهربا مش بقدر أنزل الأمان
+
+554
+00:53:54,050 --> 00:54:03,090
+ممكن جدا أقدر أنزل الأمان أو أطفل كهربا ابعد
+
+555
+00:54:03,090 --> 00:54:08,130
+المصاب عن مصدر الإصابة أو ابعد الإصابة من المصدر
+
+556
+00:54:08,130 --> 00:54:18,870
+عن الإنسانيعني مثلا واحد مولع قواعي كيف ممكن اعمل؟
+
+557
+00:54:18,870 --> 00:54:24,950
+بتطفيه كيف بتطفيه؟ لو لفيته البطانية مبللة أو
+
+558
+00:54:24,950 --> 00:54:30,070
+بطانية بدون تبليل لفته فيها بتطفي ليش بتطفي؟ مناء
+
+559
+00:54:30,070 --> 00:54:36,330
+ال oxygen والاشتعال بتمش اللي بوجود oxygen فبطفي
+
+560
+00:54:36,330 --> 00:54:43,020
+اللي هو الاشتعال كمان يعنيممكن ابعد الإنسان عن
+
+561
+00:54:43,020 --> 00:54:46,800
+الغازية السخنة اللى ممكن تسببله اختناق وممكن
+
+562
+00:54:46,800 --> 00:54:56,140
+تسببله إيش تهيأج أو تورم في منطقة التنفس المصاب
+
+563
+00:54:56,140 --> 00:55:04,540
+بكل بساطة بنسمع احنا مثل في البلدي بقولالغرجان
+
+564
+00:55:04,540 --> 00:55:10,960
+بتعلج بجشة الجشة بتحمل بني آدم؟ في كمان مثل اكتر
+
+565
+00:55:10,960 --> 00:55:16,860
+من هيك الغرجان بتعلج بحبال الهوى بمسك حبال الهوى
+
+566
+00:55:16,860 --> 00:55:19,880
+ماخدين بالكوا كده؟ بشوف كأنه في حبال بمسك فيها
+
+567
+00:55:19,880 --> 00:55:24,920
+فعشان هيك لما تيجي تلاقي واحد بده موت غالبا
+
+568
+00:55:24,920 --> 00:55:29,240
+بتلاقيه ماسك ماسك ففكر اش يمسكوا يعني بده حاجة
+
+569
+00:55:29,240 --> 00:55:32,140
+ينجحان فالغرجان
+
+570
+00:55:33,810 --> 00:55:39,030
+في الميّه لسه أخف ألمًا من الغرجان في النار هو
+
+571
+00:55:39,030 --> 00:55:45,010
+المحرج عبارة عن إيش؟ واحد حواليه نار و لا لأ فهذا
+
+572
+00:55:45,010 --> 00:55:49,510
+اللي حواليه نار يعني إيش ما جوا قدامه و بده يجري
+
+573
+00:55:49,510 --> 00:55:55,270
+ممكن يولّع حاله و ممكن يولّع اللي بده ينقذه فممكن
+
+574
+00:55:55,270 --> 00:56:00,250
+يحرجك وهذه شفناها أكتر من مرة أن يجي واحد قدام
+
+575
+00:56:00,250 --> 00:56:05,430
+حرجاتلأن والله أنا طفيت النار عن مرتي أو طفيت
+
+576
+00:56:05,430 --> 00:56:09,270
+النار عن أخويا أو طفيت النار عن بنتي أو عن ابني أو
+
+577
+00:56:09,270 --> 00:56:16,870
+عن جاري ف .. عشان هيك بدك تهدي المصاب تقولله انتبه
+
+578
+00:56:16,870 --> 00:56:23,550
+عشان أخدمك ماانتبهتش بسيبك و بشرد صحيح بسيبك و
+
+579
+00:56:23,550 --> 00:56:30,450
+بشرد فتهديت المصاب مهم جدا يعني و ..نحاول أنه يعني
+
+580
+00:56:30,450 --> 00:56:37,510
+نعطيله حاجة تخفف الأدان و نوديه على أقرب مركز طبي
+
+581
+00:56:37,510 --> 00:56:43,570
+بيعمله الإسعاف طبعا كيف أنا بدي أطف النار زي ما
+
+582
+00:56:43,570 --> 00:56:50,250
+قلنا بنلفه البطانية أو ممكن يخلينا مع الأرض و يضحك
+
+583
+00:56:50,250 --> 00:56:54,170
+يعني أنا مثلا محروق دهري لو نمت على دهري إيش
+
+584
+00:56:54,170 --> 00:56:59,150
+بيصير؟تنطفى النار لو صدره محروق ونام ع صدره ايش
+
+585
+00:56:59,150 --> 00:57:04,730
+بيصير تنطفى النار فده حرجة على منطقة النار ممكن
+
+586
+00:57:04,730 --> 00:57:11,510
+جدا و لو كان على الأرض برضه بنخنقش من الغازات لإن
+
+587
+00:57:11,510 --> 00:57:16,330
+الغازات بتطلع .. بتكون أخف فبتطلع إلى أعلى، نعم
+
+588
+00:57:16,330 --> 00:57:20,290
+هيك
+
+589
+00:57:20,290 --> 00:57:28,520
+عرفنا درجات الحروقاعرفنا نسبة الحروق، اعرفنا
+
+590
+00:57:28,520 --> 00:57:32,580
+المخاطر الضافية للحروق، فى حد عنده أي سؤال فى اللى
+
+591
+00:57:32,580 --> 00:57:38,860
+فات؟ كله ان شاء الله واضح يعنى؟ طيب ممكن كيف نسعف
+
+592
+00:57:38,860 --> 00:57:43,400
+Formally؟ هدول الناس بدهم اسعاف، احنا بندرس مادة
+
+593
+00:57:43,400 --> 00:57:48,120
+اسم ايش؟ الاسعاف الأولى، فضرورى نعرف كيف نسعف
+
+594
+00:57:48,120 --> 00:57:53,010
+البناء اللىاسعار في حرج الدرجة الأولى .. حرج
+
+595
+00:57:53,010 --> 00:58:00,630
+الدرجة الأولى كنا أبسط أنواع الحروق ويعني قد لا
+
+596
+00:58:00,630 --> 00:58:05,530
+يحتاج إلى يعني تدخل مسار لكن احنا ممكن ايش نساوم
+
+597
+00:58:05,530 --> 00:58:13,570
+.. يعني يوم من الأيام في رحلة طلعت وزي ما قلتلكم
+
+598
+00:58:13,570 --> 00:58:19,760
+الرحلات بتطلع في الصيف .. بنات .. بنتي قعدهمفروحت
+
+599
+00:58:19,760 --> 00:58:24,960
+مش متحملة منديلها على وجهها من حرارة الشمس و من
+
+600
+00:58:24,960 --> 00:58:29,080
+الحرج من الدرجة الأولى جالتلي لسه انا أخاف واحدة
+
+601
+00:58:29,080 --> 00:58:33,640
+في الرحلة اللي احنا سلم عليها مات اللي معناها مش
+
+602
+00:58:33,640 --> 00:58:39,900
+متحملة الهواء طبعا زي ما قلنا بنات صعب انهم يطلعن
+
+603
+00:58:39,900 --> 00:58:45,250
+بيطلعن في الصيف ضالن طول اليوم تحت الشمسو حرارة
+
+604
+00:58:45,250 --> 00:58:49,710
+الشمس عالية فصار عندنا حروق من الدرجة الأولى
+
+605
+00:58:49,710 --> 00:58:55,990
+بتطلعي مش متحملة تحط اشي على جسمها فإيش هذه ممكن
+
+606
+00:58:55,990 --> 00:59:01,130
+نعملها طالما كتغسلي بالعضو المصاب أو بتحطيه في ميه
+
+607
+00:59:01,130 --> 00:59:06,070
+لو دخلتيها حمام أو حطيت باني و قعدتيها فيه ممكن
+
+608
+00:59:06,070 --> 00:59:13,800
+جدا .. نعم ممكن جدا يخفف الألمالاطراف لو ارتفعت
+
+609
+00:59:13,800 --> 00:59:20,340
+الى اعلى كمان بيخف الألم و بعدين بنجفف المكان و
+
+610
+00:59:20,340 --> 00:59:28,900
+بننظفه و ممكن نحط شاشة عشان يمنع الحرارة بالنسبة
+
+611
+00:59:28,900 --> 00:59:38,340
+فاش مشكلة انك تحطي حاجة تمنع الألم يعني لو اعطيتي
+
+612
+00:59:38,340 --> 00:59:41,880
+.. اعطيتي البني آدم انا ال .. ايش بعمله دول الناس
+
+613
+00:59:42,480 --> 00:59:48,480
+بعمللهم باعطيهم برهم بنج اذركائين اسمه برهم
+
+614
+00:59:48,480 --> 00:59:55,580
+اذركائين لو حطيتي برهم اذركائين بيخفف الأدام و بعد
+
+615
+00:59:55,580 --> 01:00:01,020
+هيك ان شاء الله بتحسن الواحد اسعاف
+
+616
+01:00:01,020 --> 01:00:05,660
+حرج من الدرجة الثانية حرج الدرجة الثانية بلغوا
+
+617
+01:00:05,660 --> 01:00:11,130
+بدنا نبردبس جونا حرج الدرجة التالنية بيتصف بصفة
+
+618
+01:00:11,130 --> 01:00:16,850
+انه فيه فقاعات مائية فى فقاعات مائية معناته ايه
+
+619
+01:00:16,850 --> 01:00:24,930
+هذه الفقاعات تحتوي على سوائل لو حطينا ثلج الفقاعات
+
+620
+01:00:24,930 --> 01:00:30,810
+المائية هذه هتصير ثلجولا لأ هتنتقل برودة إلها و
+
+621
+01:00:30,810 --> 01:00:35,950
+تصير تلج إذا صار تلج هيعمل مشكلة عمرك فتحت تلاجتك
+
+622
+01:00:35,950 --> 01:00:40,750
+و الفريزر ملان تلج و مش عارف اطولي منه ولا غلط ولا
+
+623
+01:00:40,750 --> 01:00:46,230
+حاجة ففتحت التلاجة عشان يخف التلج اليوم no frost
+
+624
+01:00:46,230 --> 01:00:51,050
+يعني اليوم غالب التلاجات بدون .. بالبخار لكن قبل
+
+625
+01:00:51,050 --> 01:00:56,720
+شوية قبل يمكن سنتين زمان بقى كله تلاجات بفريزرو
+
+626
+01:00:56,720 --> 01:01:04,060
+متلج فتلاقي التلج نزل زي رؤوس الإبار لاحظته؟ هذا
+
+627
+01:01:04,060 --> 01:01:08,220
+بالظبط ممكن يصير في الجسم لما تيجي انت في فخوعات
+
+628
+01:01:08,220 --> 01:01:13,440
+مائية و تحط ثلج ممكن يصير الثلج كأنه رؤوس إبار أي
+
+629
+01:01:13,440 --> 01:01:17,920
+فرقة فيه بتموت خلايا أي فرقة فيه بتموت خلايا
+
+630
+01:01:17,920 --> 01:01:24,280
+فبتزيدالتلف في الجسم بتزيد التلف في الجسم و بتزيد
+
+631
+01:01:24,280 --> 01:01:28,860
+تأثير الحرق عشان هيك بنحطه في مي باردة الحرق من
+
+632
+01:01:28,860 --> 01:01:33,980
+الدرجة التانية الأولى مش كتير مشكلة لو حطينا ثلج
+
+633
+01:01:33,980 --> 01:01:44,880
+لكن احنا ننصح أولا بالباردة بعدين بنجفف و بنوديه
+
+634
+01:01:44,880 --> 01:01:48,420
+على وين أقرب مركز طبي إذا والله في الإصابة في
+
+635
+01:01:48,420 --> 01:01:52,760
+الأطرافبترفع الأطراف إلى أعلى في أي إصابة في
+
+636
+01:01:52,760 --> 01:01:57,300
+الأطراف إن نزلت إيدك و إيدك مضروبة بيصير تضرب عليك
+
+637
+01:01:57,300 --> 01:02:02,380
+جامد مجرد بس ما ترفع إيدك هيك بتحس سبحان الله خف
+
+638
+01:02:02,380 --> 01:02:16,400
+الأدمان زي النزيف الشيء .. زي النزيف الشيء نعم
+
+639
+01:02:16,400 --> 01:02:22,380
+و رفعت لجيالكوخف الألم الحمد لله بخف الألم الرافع
+
+640
+01:02:22,380 --> 01:02:30,800
+بخف الألم و بخف الوعي حرق الدرجة الثالثة الحين حرق
+
+641
+01:02:30,800 --> 01:02:37,840
+الدرجة الثالثة أغمق من حرق الدرجة الأولى و الثانية
+
+642
+01:02:37,840 --> 01:02:43,760
+مين فيك اللافسة أواعى مافيها سلك خيط نيلون مافيش
+
+643
+01:02:43,760 --> 01:02:49,690
+الحين و غالبا النيلون بيجيبوه من وينمن ال petrol
+
+644
+01:02:49,690 --> 01:02:57,410
+هو اقول من المشتقات ال petrol فبكل بساطة هذا سريع
+
+645
+01:02:57,410 --> 01:03:04,770
+اشتعال و بسيح يعني تعالى انت نعم نايلون هم نايلون
+
+646
+01:03:04,770 --> 01:03:10,190
+تعالى انت حط جنيه ميه مش مغطى يعنى و نصها ميه و
+
+647
+01:03:10,190 --> 01:03:14,190
+نصها التانى فاضى جنب او حوالين النار تلاقيها طابة
+
+648
+01:03:14,190 --> 01:03:22,790
+يعنى ايش بيصير فيها تنطبقليش بالمطابق؟ نعم؟
+
+649
+01:03:22,790 --> 01:03:26,890
+لو كانت ملانة ميه سخنه ملانة لا تحفه سخنت الميه
+
+650
+01:03:26,890 --> 01:03:31,730
+مابصيرش فيها اشي و ليش رايكوا؟ يعني انا اذكر ان
+
+651
+01:03:31,730 --> 01:03:39,050
+عملت شاي في جنية كوله جنية كوله plastic مابصيهاش؟
+
+652
+01:03:39,050 --> 01:03:43,410
+مابصيهاش انا بحط الكباية بتاعة الشاي هذا ال
+
+653
+01:03:43,410 --> 01:03:49,500
+plastic بتاعة البرود بعمل .. بحطها على الغازأه مين
+
+654
+01:03:49,500 --> 01:03:55,360
+عندها جراءة تتحاول و تقوللي حط دب الكباية مي للحفة
+
+655
+01:03:55,360 --> 01:03:59,840
+و حطيها فوق عين الغاز و بيملعك مابساوش يعني بصلك
+
+656
+01:03:59,840 --> 01:04:05,000
+تتسخن الكباية المي و ال .. هذا بصير تغلي أه كون
+
+657
+01:04:05,000 --> 01:04:11,220
+المي في قلبها بتسيحش الكباية أه سبحان الله
+
+658
+01:04:11,220 --> 01:04:17,620
+بتتكرمشش كون المي في قلبهاماقولكوا أنا عملت .. لما
+
+659
+01:04:17,620 --> 01:04:23,220
+روحنا عملنا عمرة أنا و زوجاتى جالت لما واحد منهم
+
+660
+01:04:23,220 --> 01:04:27,720
+بدى شاى شاى؟ شاى اجي عبالة تشرب شاى احنا نازلين
+
+661
+01:04:27,720 --> 01:04:32,620
+فندق لسه جديد و قالت والله انا الحين يعني مش يعرف
+
+662
+01:04:32,620 --> 01:04:37,340
+اتحرك بدى شاىفإيش ساويت؟ روحت جيبت سلكين كهربا و
+
+663
+01:04:37,340 --> 01:04:41,300
+جيبت بلاستيكا احنا معانا شاينا و سكرنا ميخدين و
+
+664
+01:04:41,300 --> 01:04:44,900
+ناشف و حاطيتها في الكهربا و حاطيت السلكين في الميه
+
+665
+01:04:44,900 --> 01:04:50,320
+بس تعملوش زيها لإنه مش سهل و غلطت و شربت شاي و
+
+666
+01:04:50,320 --> 01:04:54,660
+شربنا شاي ماخدين بالكوا كيف؟ قولنا ان الكهربا
+
+667
+01:04:54,660 --> 01:05:00,390
+بتعمل تأيون للميه و بتعمل حرارةفاستفدت من هذه
+
+668
+01:05:00,390 --> 01:05:05,070
+المعلومة فين عملتها فماسحتش ال plastic هاي الجثة
+
+669
+01:05:05,070 --> 01:05:12,470
+يعني اذا ال .. الاشياء ال nylon هذه بتشد على جسم
+
+670
+01:05:12,470 --> 01:05:18,370
+الإنسان فبتلزج فانت كمسعفة ماتحاوليش تسلقيها ليش
+
+671
+01:05:18,370 --> 01:05:23,630
+ماتحاوليش تسلقيها؟ لأن هذه وجودها على الجسم بيمنع
+
+672
+01:05:23,630 --> 01:05:28,780
+انزول السوائل طب وينجمناها؟بدوا يصيروا ينزلوا
+
+673
+01:05:28,780 --> 01:05:36,420
+السواق فخليها في مكانها و بعدين يغطى الجرح بطبقة
+
+674
+01:05:36,420 --> 01:05:43,080
+من الشاشة و ترفع الأطراف عن مستوى القلب و بنخل
+
+675
+01:05:43,080 --> 01:05:48,460
+المصاب يعني يهدأ لأن الحركة بدها دورة دموية و بدنا
+
+676
+01:05:48,460 --> 01:05:53,750
+نفقد الدورة الدموية في الأطرافطبعا إذا الوجه و
+
+677
+01:05:53,750 --> 01:05:58,150
+العنق و مكان التنفس واصلا الإصابة بيناهتم جدا
+
+678
+01:05:58,150 --> 01:06:04,230
+بتنفس المصاب لإنه في كل دقيقة ممكن يختنق و يسكر
+
+679
+01:06:04,230 --> 01:06:11,430
+نفسه و ممنوع ممنوع ممنوع انحط مية سواء تلج سواء
+
+680
+01:06:11,430 --> 01:06:16,450
+مية باردة سواء اي مية على الحرج من الدرجة التالتة
+
+681
+01:06:16,450 --> 01:06:18,150
+اتفضل
+
+682
+01:06:19,850 --> 01:06:22,870
+ليش بدنا عشان نحط ميه باردة على الحرق من الدرجة
+
+683
+01:06:22,870 --> 01:06:28,230
+التالتة، ممنوع، بتسبب مصيب زيادة ومشكلة زيادة،
+
+684
+01:06:28,230 --> 01:06:35,690
+ليش؟ لإن اللي .. اللي جوا خارجنا .. كنت
+
+685
+01:06:35,690 --> 01:06:41,950
+روحت غمرك على البحر وخشيت في الميه، قعدتيلك ساعة
+
+686
+01:06:41,950 --> 01:06:51,030
+زمان، نص ساعة، ايش بطلع في جدك؟ ايش يعني بكرمش؟إيش
+
+687
+01:06:51,030 --> 01:06:57,110
+السبب إنه بكرمش؟ لما بنتحمل مثلا في طبقة بتطلع
+
+688
+01:06:57,110 --> 01:07:00,950
+عشان تمنع يعني عشان تحفظ المي اللي جوا الإصفة حد
+
+689
+01:07:00,950 --> 01:07:08,250
+هي بتكرمش لش
+
+690
+01:07:08,250 --> 01:07:14,010
+بكرمش؟ ليش الجلد بنشف؟ عشان الضغط الأوزموزي، أيش
+
+691
+01:07:14,010 --> 01:07:18,810
+الضغط الأوزموزي؟ صح كلامك، إيش بيسير؟
+
+692
+01:07:21,390 --> 01:07:25,450
+البحر تركيزه أعلى من تركيز الجسم فبصير جال البحر
+
+693
+01:07:25,450 --> 01:07:29,810
+بده يعادل نفسه من سوائل جسمي فبصيره مصف سوائلي
+
+694
+01:07:29,810 --> 01:07:35,290
+فبفقدني سوائل بصير عندي جفافة عشان هيك بتروحي على
+
+695
+01:07:35,290 --> 01:07:39,550
+البحر كل شوية بتخش المي بترجع بدك تشربيه مش
+
+696
+01:07:39,550 --> 01:07:45,450
+فاريجيك من العطش مظبوط؟ ليش؟ لأن المي بتفوق ..
+
+697
+01:07:45,450 --> 01:07:49,030
+بتسحب .. فلو جيتي واحد محروق درجة تالتة وصبيت عليه
+
+698
+01:07:49,030 --> 01:07:55,470
+ميههو مصيبة لدرجة ثالثة انه بيفقد سوائل و مع ايش؟
+
+699
+01:07:55,470 --> 01:08:00,850
+مع الاملاح فالحين لو حاطينا عليها ميه بتسير الميه
+
+700
+01:08:00,850 --> 01:08:05,990
+مص املاح من داخل الجسم وسوائل كمان فسوّت مصيبة
+
+701
+01:08:05,990 --> 01:08:11,850
+زيادة سوّت مشاكل زيادة للانسان فإذا لا يوضع ماء
+
+702
+01:08:11,850 --> 01:08:17,590
+مطلقا انما ايش بنعمل في الميه بنعطيله ميه مع
+
+703
+01:08:17,590 --> 01:08:23,010
+كربونة وملحعشان انعوضله السوائل عن طريق الفم اذا
+
+704
+01:08:23,010 --> 01:08:27,890
+بيشرب اذا كان بيشرب كيف طبعا معلجه الى نص على
+
+705
+01:08:27,890 --> 01:08:35,810
+اللتر عشان انعوضله السوائل لا يقطع المريض اي مادة
+
+706
+01:08:35,810 --> 01:08:39,010
+كحولي لان طبعا ما جعل الله شفاء أمتي في ما حرم
+
+707
+01:08:39,010 --> 01:08:42,170
+عليهم او في نجاس كما قال صلى الله عليه و سلم
+
+708
+01:08:42,170 --> 01:08:46,610
+فالحرام الكحول الحاجة التانية في الحادات الحرج
+
+709
+01:08:47,080 --> 01:08:50,840
+بتبقى المعدة أصلا متهيجة فلمّا تعطيها كهول بتنفجر
+
+710
+01:08:50,840 --> 01:08:57,680
+وبتسبب تقرحات نازفة و هذا طبعا يعني بخشنة في
+
+711
+01:08:57,680 --> 01:09:05,240
+المشاكل أكتر الحين زي ما في حرق بالمواد النار و
+
+712
+01:09:05,240 --> 01:09:10,580
+المشتقاتها و الأشياء الحارة في حروق كيميائي وقول
+
+713
+01:09:10,580 --> 01:09:15,790
+النضار ملانا أشياء كيميائية ولا لأالمادة
+
+714
+01:09:15,790 --> 01:09:18,610
+الكيميائية حرق من المادة بالمادة الكيميائية خيط
+
+715
+01:09:18,610 --> 01:09:24,390
+كيف بدي اتعامل معاه واحد اجعل جسمه ميط نار ايش بدي
+
+716
+01:09:24,390 --> 01:09:29,250
+اعمل فيه بدي
+
+717
+01:09:29,250 --> 01:09:36,750
+ابعده عن ميط النار او ابعد ميط النار عنه كيف بدي
+
+718
+01:09:36,750 --> 01:09:42,550
+اظل اغسل بمي جارية بدي اظل اغسل بمي جارية اذا لابس
+
+719
+01:09:42,550 --> 01:09:48,680
+أواعن تلت من ال ..بغير هذه الملابس بغير الملابس و
+
+720
+01:09:48,680 --> 01:09:56,740
+بغسل بالماء و الصابون كمان بعمل عملية معادلة مين
+
+721
+01:09:56,740 --> 01:10:07,340
+عمرها فرمة فلفل لما
+
+722
+01:10:07,340 --> 01:10:12,080
+بتعملي فلفل طبعا انا اعملت تجربة على الفلفل هذا
+
+723
+01:10:12,080 --> 01:10:15,840
+كان الدورما شوفت اللى بنت ابني جاية يا سيده ده
+
+724
+01:10:15,840 --> 01:10:21,080
+يولعني وقولت بس يا سيده أحسن فرصة أعمل تجربة أعرف
+
+725
+01:10:21,080 --> 01:10:26,580
+الفلفل حامض ولا قلون أعرف الفلفل حامض ولا قلون مش
+
+726
+01:10:26,580 --> 01:10:30,580
+مادة كيميائية الفلفل فالمادة الكيميائية غسلنا
+
+727
+01:10:30,580 --> 01:10:37,620
+الأيدان طلت تحرك وقولت بس جبت حطيت مثلا شوية
+
+728
+01:10:37,620 --> 01:10:44,170
+كربونة ع نطفة ميه ودرتها زاد زاد؟ اه زادفروحت حطيت
+
+729
+01:10:44,170 --> 01:10:51,950
+لمون طابت اه لمون على الفلفل انتوا بتلاحظوا انه
+
+730
+01:10:51,950 --> 01:10:58,910
+لما تقصر لمون على الفلفل بتحسيش بحرجانه اه لاحظتوا
+
+731
+01:10:58,910 --> 01:11:03,770
+ولا بلاحظتوا لما تيجي تاكل و تقصر لمون على الفلفل
+
+732
+01:11:03,770 --> 01:11:08,570
+ايش بيصير فيه بيصير ذاكي و بيخف حرجانه مظبوط ولا
+
+733
+01:11:08,570 --> 01:11:16,020
+لأالحرج احنا بنجيب الفلفل و هذا كحاجة يعني تقولك
+
+734
+01:11:16,020 --> 01:11:22,820
+انه في حاجة في حال وجود مادة كيميائية الجسم بتخلصش
+
+735
+01:11:22,820 --> 01:11:28,120
+تماما بالغسيل من المادة الكيميائية بدليل ان الفلفل
+
+736
+01:11:28,120 --> 01:11:34,420
+بعد تلت ساعات او اربع ساعات بتمسح عينك بتولع صح؟ و
+
+737
+01:11:34,420 --> 01:11:38,020
+انت بتبقى قاعدة في الدار مش حاسة بحرجان الفلفل ولا
+
+738
+01:11:38,020 --> 01:11:44,570
+حاسةالفلفل الفلفل الفلفل الفلفل الفلفل الفلفل
+
+739
+01:11:44,570 --> 01:11:44,990
+الفلفل الفلفل الفلفل الفلفل الفلفل الفلفل الفلفل
+
+740
+01:11:44,990 --> 01:11:46,390
+الفلفل الفلفل الفلفل الفلفل الفلفل الفلفل الفلفل
+
+741
+01:11:46,390 --> 01:11:49,930
+الفلفل الفلفل الفلفل الفلفل الفلفل الفلفل الفلفل
+
+742
+01:11:49,930 --> 01:11:53,070
+الفلفل الفلفل الفلفل الفلفل الفلفل الفلفل الفلفل
+
+743
+01:11:53,070 --> 01:11:56,210
+الفلفل
+
+744
+01:11:56,210 --> 01:11:57,670
+الفلفل الفلفل الفلفل الفلفل الفلفل الفلفل الفلفل
+
+745
+01:11:57,670 --> 01:12:00,870
+الفلفل الفلفل
+
+746
+01:12:00,870 --> 01:12:04,490
+الفلفل
+
+747
+01:12:04,490 --> 01:12:07,590
+الفلفل
+
+748
+01:12:07,590 --> 01:12:14,980
+الفأحط للحمض قلوي و القلوي حامض بحطش حامض مضر و لا
+
+749
+01:12:14,980 --> 01:12:19,240
+قلوي مضر يعني أنا بحطش صودة كاوية اللي هي القلوي
+
+750
+01:12:19,240 --> 01:12:25,920
+لميط النار وهذه بتذبح وهذه بتذبح إنما بحط أشياء
+
+751
+01:12:25,920 --> 01:12:30,480
+طبيعية بأكلها في الدار يعني عندي في الدار أنا قلوي
+
+752
+01:12:30,480 --> 01:12:34,280
+مثلا الكربونة بحطها على الفلافل بحطها على بعض
+
+753
+01:12:34,280 --> 01:12:40,810
+المعجناتبحطها على حاجات كتير و لا لأ فلو جيبنا على
+
+754
+01:12:40,810 --> 01:12:46,290
+الحامض معلجة كربونة على كباية ميه و خلطنا و
+
+755
+01:12:46,290 --> 01:12:50,390
+حطيناها على المنطقة بعد ما نغسلها بتعادل المادة
+
+756
+01:12:50,390 --> 01:12:53,970
+الحامض و في الدار عندى إيش مادة حامضة بستخدمها
+
+757
+01:12:53,970 --> 01:12:59,870
+انا؟ الخلو الخلو و الليمون الخلو و الليمون باكله
+
+758
+01:12:59,870 --> 01:13:03,030
+بحطه على الطبيقه و بحطه على الصلطة بتحط الخلو على
+
+759
+01:13:03,030 --> 01:13:03,350
+الصلطة
+
+760
+01:13:06,620 --> 01:13:13,900
+نحط خل على الصينة نحطها .. نحطها .. نحطها .. نحطها
+
+761
+01:13:13,900 --> 01:13:13,980
+.. نحطها .. نحطها .. نحطها .. نحطها .. نحطها ..
+
+762
+01:13:13,980 --> 01:13:14,020
+نحطها .. نحطها .. نحطها .. نحطها .. نحطها .. نحطها
+
+763
+01:13:14,020 --> 01:13:16,320
+.. نحطها .. نحطها .. نحطها .. نحطها .. نحطها ..
+
+764
+01:13:16,320 --> 01:13:17,000
+نحطها .. نحطها .. نحطها .. نحطها .. نحطها .. نحطها
+
+765
+01:13:17,000 --> 01:13:18,480
+.. نحطها .. نحطها .. نحطها .. نحطها .. نحطها ..
+
+766
+01:13:18,480 --> 01:13:23,240
+نحطها .. نحطها .. نحطها .. نحطها .. نحطها .. نحطها
+
+767
+01:13:23,240 --> 01:13:34,040
+.. نحطها .. نحطها .. نحطها .. نحطها .. نحطها ..
+
+768
+01:13:34,040 --> 01:13:43,130
+نحطها ..وإذا لزم غيار بنحط غيار سكه؟
+
+769
+01:13:43,130 --> 01:13:47,250
+مش عارف للفلفل؟
+
+770
+01:13:47,250 --> 01:13:52,730
+والله أنا بنعرف أما أنا بس جربت .. جربت تجربة إن
+
+771
+01:13:52,730 --> 01:13:58,130
+حطيت على الفلفل لمون راح يعني حطيت مادة حاملة
+
+772
+01:13:58,130 --> 01:14:04,270
+بمعنى أنه طلع قلوي فلو حطيتي لمون أو حطيتي خال
+
+773
+01:14:04,270 --> 01:14:10,940
+مكسورممكن، يمكن السكر، ممكن، ليش لأ؟ لو واحد أجى
+
+774
+01:14:10,940 --> 01:14:16,760
+مادة حارقة في عينه ميط
+
+775
+01:14:16,760 --> 01:14:23,980
+نار، ماشي؟ واحد أجت ميط نار في عينه كيف أنا بدي
+
+776
+01:14:23,980 --> 01:14:32,980
+أتعامل مع حريقين عيني؟ الغسل بالماء الجارىالغسل
+
+777
+01:14:32,980 --> 01:14:38,800
+بالماء الجارى العلاجي الأمثل في حالة الإسعاف بجيب
+
+778
+01:14:38,800 --> 01:14:43,440
+مية و بدير طبعا مية جارية مش بجيب حنافية جاوية و
+
+779
+01:14:43,440 --> 01:14:51,180
+بصير أضخ لأن العين مضروبة جنية إبريق شاث بدبوا مية
+
+780
+01:14:51,180 --> 01:14:54,260
+و بصير أدير دير في العين العين قداش بالتسعة في
+
+781
+01:14:54,260 --> 01:14:59,150
+كركوب مقطينلو جيت تجطر تحطي تلقوا طين جطرة واحد
+
+782
+01:14:59,150 --> 01:15:03,190
+بتطلع برا واحد بتضجه هو مظبوط؟ مانتساش كتير فشويه
+
+783
+01:15:03,190 --> 01:15:08,910
+شويه بدير بس كيف الفن في دير المي في العين؟ كيف
+
+784
+01:15:08,910 --> 01:15:16,350
+بدي أدير؟ بخليها مفتوحة و بنرفع رموش لكن كيف بدي
+
+785
+01:15:16,350 --> 01:15:21,890
+أحط وجهه؟ بدي أحط وجهه على الشجة المصابة يعني هذه
+
+786
+01:15:21,890 --> 01:15:26,100
+العين مضروبة بدي أدير هيك و أدير ديرلو هذه العين
+
+787
+01:15:26,100 --> 01:15:31,480
+مضرب ودرت هيك هينتجل منها لعين التانية فلأ بدي
+
+788
+01:15:31,480 --> 01:15:36,360
+أكون على الجهة المصابة و أدير الميه طبعا انا أنصح
+
+789
+01:15:36,360 --> 01:15:40,380
+جدا جدا اذا في مشكلة من هذا النوع حتى في السيارة
+
+790
+01:15:40,380 --> 01:15:43,600
+اللي بتنجل الحالة اتضلك في الطريق تدير الميه
+
+791
+01:15:43,600 --> 01:15:49,030
+هنديكم مثلخلّينا نقول عجراننا كده شخص شفنا في
+
+792
+01:15:49,030 --> 01:15:53,450
+هالدنيا معلش اسأل التاريخ اللي فيه العبر نظر لقومه
+
+793
+01:15:53,450 --> 01:15:58,490
+ليس يدرون الخبر الواحد كل ما يعني عاش أكتر بصير
+
+794
+01:15:58,490 --> 01:16:02,910
+عنده خبرة في الحياة أكتر إذا بده وإذا بدهوش بنزوي
+
+795
+01:16:02,910 --> 01:16:10,330
+نظر في دارهم، مش هيك؟ في عندنا جار جارنا هو بيشتغل
+
+796
+01:16:10,330 --> 01:16:15,870
+مع قصير عارفين اللي صار كيف؟ شمانته وشيت
+
+797
+01:16:18,160 --> 01:16:26,760
+وميه فشيد مادة كاوي اجت مقطة اقصارة في عين الراجل
+
+798
+01:16:26,760 --> 01:16:31,760
+طبعا هو ساكن في الصراعات حملوه و ادوه على مستشفى
+
+799
+01:16:31,760 --> 01:16:38,640
+العيون مستشفى العيون جنب الأطفال في النصر وصلت
+
+800
+01:16:38,640 --> 01:16:46,390
+عينه مطفيه الببه هذا تبع العين لها رايحأبيض هالعين
+
+801
+01:16:46,390 --> 01:16:53,730
+أبيض فش واخدين بالكوا كيف في المقابل أحد طلاب
+
+802
+01:16:53,730 --> 01:16:57,830
+الجامعة الإسلامية برضه من الفدائية رشو في عينه ميط
+
+803
+01:16:57,830 --> 01:17:03,850
+نار واخدين بالكوا كيف أيام مشاكل أيام ما كان
+
+804
+01:17:03,850 --> 01:17:09,330
+اليهود عندنا رشو في عينه ميط نار طبعا في الجامعة
+
+805
+01:17:09,330 --> 01:17:14,810
+هو ان عمل الإسعاف الأولي بفضل الله صحيحو هاي أخونا
+
+806
+01:17:14,810 --> 01:17:19,770
+بيشوف هو معاه دكتوره و بدرس في الجامعة ماخدين
+
+807
+01:17:19,770 --> 01:17:25,750
+بالكوا كيف؟ و هي ميتناه ليش؟ الله يسر أنه كان في
+
+808
+01:17:25,750 --> 01:17:31,710
+مكان جريب و أسعف بسرعة في الطريقة الصحية ماخدين
+
+809
+01:17:31,710 --> 01:17:37,830
+بالكوا كيف؟ فإنتي بسرعة ضلك ديري مية حتى في الطريق
+
+810
+01:17:37,830 --> 01:17:41,950
+إلى المستشفى لإرادة واحد سببته ميةأه بس انتوا بعض
+
+811
+01:17:41,950 --> 01:17:44,890
+الناس بيغطيها و بيصيبها خلاص و بيضلوا يوحى توكل
+
+812
+01:17:44,890 --> 01:17:51,070
+فيه و هو ماعوش خبر، لأ لما نضلنا نغسل فيها إلى أنه
+
+813
+01:17:51,070 --> 01:17:56,430
+إيش نصل إلى أقرب مركز طبي ان شاء الله
+
diff --git a/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/ToF1ruxgrGo_raw.json b/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/ToF1ruxgrGo_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..18ea0e8debb3d7714c7b49b125f7a0fe77ee7fa7
--- /dev/null
+++ b/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/ToF1ruxgrGo_raw.json
@@ -0,0 +1 @@
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47283, 3215, 9957, 20666, 4117, 14407, 9122, 33911, 22807, 6156, 28814, 29399, 1829, 4724, 3794, 2288, 27884, 48812, 23275, 11778, 13546, 1829, 3714, 10632, 11331, 49975, 8978, 41950, 16572, 4587, 30731, 9673, 14851, 8592, 5172, 7578, 5296, 28814, 2288, 18513, 3660, 36764, 24401, 8608, 3555, 3555, 47395, 3714, 10632], "avg_logprob": -0.1738591208344414, "compression_ratio": 1.9862385321100917, "no_speech_prob": 5.960464477539062e-07, "words": [{"start": 4633.87, "end": 4634.11, "word": "و", "probability": 0.9384765625}, {"start": 4634.11, "end": 4634.31, "word": " هاي", "probability": 0.4425048828125}, {"start": 4634.31, "end": 4634.81, "word": " أخونا", "probability": 0.9189453125}, {"start": 4634.81, "end": 4635.43, "word": " بيشوف", "probability": 0.9609375}, {"start": 4635.43, "end": 4636.13, "word": " هو", "probability": 0.53173828125}, {"start": 4636.13, "end": 4636.37, "word": " معاه", "probability": 0.79150390625}, {"start": 4636.37, "end": 4637.23, "word": " دكتوره", "probability": 0.90576171875}, {"start": 4637.23, "end": 4637.43, "word": " و", "probability": 0.8125}, {"start": 4637.43, "end": 4637.81, "word": " بدرس", "probability": 0.84423828125}, {"start": 4637.81, "end": 4637.91, "word": " في", "probability": 0.74609375}, {"start": 4637.91, "end": 4638.41, "word": " الجامعة", "probability": 0.97314453125}, {"start": 4638.41, "end": 4639.77, "word": " ماخدين", "probability": 0.8565673828125}, {"start": 4639.77, "end": 4640.09, "word": " بالكوا", "probability": 0.8759765625}, {"start": 4640.09, "end": 4640.57, "word": " كيف؟", "probability": 0.8614908854166666}, {"start": 4640.57, "end": 4640.97, "word": " و", "probability": 0.64697265625}, {"start": 4640.97, "end": 4641.07, "word": " هي", "probability": 0.59765625}, {"start": 4641.07, "end": 4642.35, "word": " ميتناه", "probability": 0.875244140625}, {"start": 4642.35, "end": 4644.11, "word": " ليش؟", "probability": 0.8961588541666666}, {"start": 4644.11, "end": 4644.45, "word": " الله", "probability": 0.8798828125}, {"start": 4644.45, "end": 4644.97, "word": " يسر", "probability": 0.9306640625}, {"start": 4644.97, "end": 4645.41, "word": " أنه", "probability": 0.7000732421875}, {"start": 4645.41, "end": 4645.61, "word": " كان", "probability": 0.982421875}, {"start": 4645.61, "end": 4645.75, "word": " في", "probability": 0.9501953125}, {"start": 4645.75, "end": 4646.07, "word": " مكان", "probability": 0.993896484375}, {"start": 4646.07, "end": 4646.63, "word": " جريب", "probability": 0.8732096354166666}, {"start": 4646.63, "end": 4647.33, "word": " و", "probability": 0.90673828125}, {"start": 4647.33, "end": 4647.97, "word": " أسعف", "probability": 0.935791015625}, {"start": 4647.97, "end": 4649.09, "word": " بسرعة", "probability": 0.9879150390625}, {"start": 4649.09, "end": 4649.71, "word": " في", "probability": 0.736328125}, {"start": 4649.71, "end": 4650.13, "word": " الطريقة", "probability": 0.96337890625}, {"start": 4650.13, "end": 4650.65, "word": " الصحية", "probability": 0.9103190104166666}, {"start": 4650.65, "end": 4651.71, "word": " ماخدين", "probability": 0.934326171875}, {"start": 4651.71, "end": 4651.99, "word": " بالكوا", "probability": 0.9894205729166666}, {"start": 4651.99, "end": 4652.25, "word": " كيف؟", "probability": 0.96435546875}, {"start": 4652.25, "end": 4652.91, "word": " فإنتي", "probability": 0.67913818359375}, {"start": 4652.91, "end": 4653.43, "word": " بسرعة", "probability": 0.9903564453125}, {"start": 4653.43, "end": 4654.57, "word": " ضلك", "probability": 0.5262451171875}, {"start": 4654.57, "end": 4655.01, "word": " ديري", "probability": 0.7368977864583334}, {"start": 4655.01, "end": 4655.43, "word": " مية", "probability": 0.767333984375}, {"start": 4655.43, "end": 4656.29, "word": " حتى", "probability": 0.95361328125}, {"start": 4656.29, "end": 4657.39, "word": " في", "probability": 0.97314453125}, {"start": 4657.39, "end": 4657.83, "word": " الطريق", "probability": 0.978515625}, {"start": 4657.83, "end": 4658.01, "word": " إلى", "probability": 0.88232421875}, {"start": 4658.01, "end": 4658.49, "word": " المستشفى", "probability": 0.9232421875}, {"start": 4658.49, "end": 4659.17, "word": " لإرادة", "probability": 0.7978515625}, {"start": 4659.17, "end": 4659.37, "word": " واحد", "probability": 0.8408203125}, {"start": 4659.37, "end": 4659.95, "word": " سببته", "probability": 0.66357421875}, {"start": 4659.95, "end": 4660.25, "word": " مية", "probability": 0.94091796875}], "temperature": 1.0}, {"id": 168, "seek": 467643, "start": 4660.77, "end": 4676.43, "text": "أه بس انتوا بعض الناس بيغطيها و بيصيبها خلاص و بيضلوا يوحى توكل فيه و هو ماعوش خبر، لأ لما نضلنا نغسل فيها إلى أنه إيش نصل إلى أقرب مركز طبي ان شاء الله", "tokens": [10721, 3224, 4724, 3794, 16472, 2655, 14407, 45030, 11242, 2423, 8315, 3794, 4724, 1829, 17082, 9566, 1829, 11296, 4032, 4724, 1829, 9381, 1829, 3555, 11296, 16490, 1211, 33546, 4032, 4724, 1829, 11242, 1211, 14407, 7251, 2407, 5016, 7578, 6055, 2407, 28820, 8978, 3224, 4032, 31439, 19446, 3615, 2407, 8592, 16490, 26890, 12399, 5296, 10721, 5296, 15042, 8717, 11242, 1211, 8315, 8717, 17082, 3794, 1211, 8978, 11296, 30731, 14739, 3224, 11933, 1829, 8592, 8717, 36520, 30731, 5551, 4587, 25513, 3714, 31747, 11622, 23032, 21292, 16472, 13412, 16606, 21984], "avg_logprob": -0.1790660543536598, "compression_ratio": 1.6625766871165644, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 4660.77, "end": 4661.17, "word": "أه", "probability": 0.64599609375}, {"start": 4661.17, "end": 4661.37, "word": " بس", "probability": 0.972900390625}, {"start": 4661.37, "end": 4661.73, "word": " انتوا", "probability": 0.7283528645833334}, {"start": 4661.73, "end": 4661.95, "word": " بعض", "probability": 0.974853515625}, {"start": 4661.95, "end": 4662.19, "word": " الناس", "probability": 0.9873046875}, {"start": 4662.19, "end": 4662.71, "word": " بيغطيها", "probability": 0.9593912760416666}, {"start": 4662.71, "end": 4662.81, "word": " و", "probability": 0.82763671875}, {"start": 4662.81, "end": 4663.23, "word": " بيصيبها", "probability": 0.9471028645833334}, {"start": 4663.23, "end": 4663.63, "word": " خلاص", "probability": 0.9822591145833334}, {"start": 4663.63, "end": 4663.79, "word": " و", "probability": 0.88134765625}, {"start": 4663.79, "end": 4664.15, "word": " بيضلوا", "probability": 0.9107421875}, {"start": 4664.15, "end": 4664.45, "word": " يوحى", "probability": 0.861572265625}, {"start": 4664.45, "end": 4664.89, "word": " توكل", "probability": 0.705810546875}, {"start": 4664.89, "end": 4665.25, "word": " فيه", "probability": 0.980712890625}, {"start": 4665.25, "end": 4665.39, "word": " و", "probability": 0.98583984375}, {"start": 4665.39, "end": 4665.49, "word": " هو", "probability": 0.389404296875}, {"start": 4665.49, "end": 4665.69, "word": " ماعوش", "probability": 0.8282470703125}, {"start": 4665.69, "end": 4667.21, "word": " خبر،", "probability": 0.7606608072916666}, {"start": 4667.21, "end": 4668.49, "word": " لأ", "probability": 0.757080078125}, {"start": 4668.49, "end": 4668.75, "word": " لما", "probability": 0.3763427734375}, {"start": 4668.75, "end": 4669.05, "word": " نضلنا", "probability": 0.8336181640625}, {"start": 4669.05, "end": 4669.51, "word": " نغسل", "probability": 0.990478515625}, {"start": 4669.51, "end": 4669.95, "word": " فيها", "probability": 0.9892578125}, {"start": 4669.95, "end": 4670.65, "word": " إلى", "probability": 0.7587890625}, {"start": 4670.65, "end": 4671.07, "word": " أنه", "probability": 0.7099609375}, {"start": 4671.07, "end": 4671.43, "word": " إيش", "probability": 0.7269694010416666}, {"start": 4671.43, "end": 4673.51, "word": " نصل", "probability": 0.864501953125}, {"start": 4673.51, "end": 4673.81, "word": " إلى", "probability": 0.93017578125}, {"start": 4673.81, "end": 4674.17, "word": " أقرب", "probability": 0.9607747395833334}, {"start": 4674.17, "end": 4674.75, "word": " مركز", "probability": 0.98193359375}, {"start": 4674.75, "end": 4675.69, "word": " طبي", "probability": 0.97607421875}, {"start": 4675.69, "end": 4676.01, "word": " ان", "probability": 0.6689453125}, {"start": 4676.01, "end": 4676.27, "word": " شاء", "probability": 0.98583984375}, {"start": 4676.27, "end": 4676.43, "word": " الله", "probability": 0.96533203125}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 4678.635, "duration_after_vad": 4490.948124999999}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/o7VV4VyEA2k.srt b/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/o7VV4VyEA2k.srt
new file mode 100644
index 0000000000000000000000000000000000000000..50c8ba215892f2b2d309ae429210d1427230994e
--- /dev/null
+++ b/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/o7VV4VyEA2k.srt
@@ -0,0 +1,2439 @@
+1
+00:00:18,850 --> 00:00:22,810
+الله الرحمن الرحيم إن شاء الله اليوم هنبدأ في
+
+2
+00:00:22,810 --> 00:00:27,170
+تكميل اللي أخدناه الدور الفاتح هنبدأ في موضوع
+
+3
+00:00:27,170 --> 00:00:29,090
+التشنجات
+
+4
+00:01:04,460 --> 00:01:11,820
+ماشي الحال؟ لو سألنا سؤال إيش يعني إيه التشنجات؟
+
+5
+00:01:11,820 --> 00:01:16,800
+والله إيش مال فلان؟ والله بتشنج إيش يعني بتشنج؟ مش
+
+6
+00:01:16,800 --> 00:01:23,840
+عارف منكم شوفته؟ شد عضلي، جوي، تقلصات عضلية إيش 
+
+7
+00:01:23,840 --> 00:01:27,880
+كمان؟
+
+8
+00:01:27,880 --> 00:01:37,600
+بتشنج واحد بفقد وعيه وكل عضلاته مشدودة أو متقلصة في
+
+9
+00:01:37,600 --> 00:01:44,280
+حركات لا إرادية تشنجات عبارة عن حركات لا إرادية
+
+10
+00:01:44,280 --> 00:01:54,300
+ليس لها هدف وظيفي هذه اسمها تشنجات ماشي؟ حركات لا 
+
+11
+00:01:54,300 --> 00:01:58,880
+إرادية ليس لها هدف وظيفي غالبا بتكون مصحوبة
+
+12
+00:01:58,880 --> 00:02:06,820
+بفقدان الوعي أو تشوه الوعي وممكن تحدث للإنسان فجأة
+
+13
+00:02:06,820 --> 00:02:13,740
+طبعا إيش أسباب هذه التشنجات؟ التشنجات هذه بتصير
+
+14
+00:02:13,740 --> 00:02:20,280
+نتيجة غالبا أمراض نتيجة أمراض يعني بيصير واحد مريض
+
+15
+00:02:20,280 --> 00:02:27,380
+لما يمرض ترتفع حرارته ترتفع حرارته بيصير عنده إيش؟
+
+16
+00:02:27,380 --> 00:02:35,040
+تشنج ليش؟ خاصة من؟ الأطفال خاصة الأطفال لما بترتفع
+
+17
+00:02:35,040 --> 00:02:42,280
+حرارته بتلاقي إيش صار يتشنج كمان لو يجينا ضربنا
+
+18
+00:02:42,280 --> 00:02:50,300
+واحد في رأسه نتيجة حادث سيارة سقوط من علو شجار
+
+19
+00:02:50,300 --> 00:02:56,760
+رياضة عنيفة أكل هوا وين؟ في رأسه اللي هوى في الراس
+
+20
+00:02:56,760 --> 00:03:03,500
+هذا إذا أثرت على المخ ممكن تعمل ما يسمى بالتشنجات و
+
+21
+00:03:03,500 --> 00:03:08,460
+ممكن تحصل نتيجة واحد عنده جلطة في المخ أو نزيف في 
+
+22
+00:03:08,460 --> 00:03:12,020
+المخ تسمعوا والله مال الحجة كان قالك صار عندها
+
+23
+00:03:12,020 --> 00:03:17,260
+إيش؟ جلطة في المخ أو نزيف في المخ ممكن جلطة في
+
+24
+00:03:17,260 --> 00:03:22,580
+المخ أو نزيف في المخ عند بعض الحالات تؤدي إلى إيه؟
+
+25
+00:03:22,580 --> 00:03:27,220
+تشنجة يعني أنا لما صار عندي نزيف في المخ قعدت سنة و
+
+26
+00:03:27,220 --> 00:03:33,240
+نص كاملة أخد علاج صار عندي نزيف في المخ أخد علاج
+
+27
+00:03:33,240 --> 00:03:39,800
+عشان ما يصيرش عندي تشنجات لأن أي خلل في المخ ممكن
+
+28
+00:03:39,800 --> 00:03:46,240
+يعمل تشنجات أي نزيف في المخ ممكن يعمل تشنجات أي
+
+29
+00:03:46,240 --> 00:03:51,280
+مرض في المخ ممكن يعمل ما يسمى بالتشنجات
+
+30
+00:03:58,430 --> 00:04:03,610
+إيش بالظبط اللي بيصير في حكاية التشنجات تعين
+
+31
+00:04:03,610 --> 00:04:11,330
+التشنجات بتلاقي شد جسمه في الأول وبعدين بدأ كل
+
+32
+00:04:11,330 --> 00:04:18,010
+الجسم يهتز بدأ كل الجسم يهتز و بيفقد الوعي أنا
+
+33
+00:04:18,010 --> 00:04:22,130
+شوفت ناس بتشنجوا شفتوا سرير المستشفى أكيد زورتوا
+
+34
+00:04:22,130 --> 00:04:28,450
+هذا في المستشفى قد إيش السرير المستشفى كبير ضخم كنت
+
+35
+00:04:28,450 --> 00:04:34,130
+أشوف حدا بتشنج فوق السرير يصير نفس السرير يهتز من
+
+36
+00:04:34,130 --> 00:04:38,930
+شدة فالناس هدول اللي بتشنجوا بيكون عندهم يعني قوة
+
+37
+00:04:38,930 --> 00:04:44,010
+عجيبة جدا فهذه كلها طبعا هو بيكونش الإنسان قاصدها
+
+38
+00:04:44,010 --> 00:04:49,530
+بيكونش قاصده يعمل هذه الحركات إلا أنه بتصير لا
+
+39
+00:04:49,530 --> 00:04:57,820
+إرادية نتيجة خلل في وين في خلايا المخ طبعا بيغيب
+
+40
+00:04:57,820 --> 00:05:03,260
+الواحد فبيصير متحكمش في نفسه ممكن يتبول يصير عنده
+
+41
+00:05:03,260 --> 00:05:09,000
+إخراج ممكن يصير يخرج زبد من تِمه تشوفه قالك فيلم
+
+42
+00:05:09,000 --> 00:05:15,820
+بيزبد بيطلع من تِمه رغوة بيبدأ وجهه وشفتينه يزرجوا
+
+43
+00:05:15,820 --> 00:05:21,700
+لأنه بيصير مشكلة في التنفس وبعدين هذه الأمراض
+
+44
+00:05:21,700 --> 00:05:27,320
+غالبا غالبا في معظم الحالات بتبدأ إيش اتزول يعني
+
+45
+00:05:27,320 --> 00:05:31,760
+اللي بتشنج وهدول كثير بعرف ناس بيزيدش التشنج عنده
+
+46
+00:05:31,760 --> 00:05:37,500
+عن خمس دقايق خمس دقايق وبعدها إيش بتلاقيه بروح أو
+
+47
+00:05:37,500 --> 00:05:42,360
+بيروح عنه التشنج إلا أنه في نوع من الناس في
+
+48
+00:05:42,360 --> 00:05:48,320
+عندهم بيظل تشنج لمدة طويلة وهذا بيكون السبب يعني
+
+49
+00:05:48,320 --> 00:05:54,900
+في خلل في المخ دائم كيف نصحف هدول الناس؟ نعملهم
+
+50
+00:05:54,900 --> 00:06:02,040
+إسعاف كيف؟ إيش المشكلة عندهم بهم؟ إنهم مش صاحين مش
+
+51
+00:06:02,040 --> 00:06:09,900
+صاحين يبقى ممكن إيش؟ يؤذي نفسه بأن لسانه يسكر نفسه
+
+52
+00:06:09,900 --> 00:06:14,480
+فبدأ اهتم في التنفس ممكن بدل ما يرجع لسانه يقدم
+
+53
+00:06:14,480 --> 00:06:21,360
+قدام ولا مش ممكن لو طلع لسانه قدام إيش بيصير فيه؟
+
+54
+00:06:22,180 --> 00:06:25,960
+ممكن يقطع لسانه لأنه مش هادي على حاله زيادة عن
+
+55
+00:06:25,960 --> 00:06:30,760
+اللزوم فبدي أمنعه يعض لسانه أو أمنع أنه يقطع لسانه
+
+56
+00:06:30,760 --> 00:06:34,400
+كيف بدي أمنع أنه يقطع لسانه؟ أي حاجة بتجيبيها
+
+57
+00:06:34,400 --> 00:06:43,500
+تحطيها بين أسنانه ها قد معص بيظل لساني إياش مجال
+
+58
+00:06:43,500 --> 00:06:51,600
+أنه ما ينقصش ما ينقصش كمان بيظل أسهل لطريقة التنفس من
+
+59
+00:06:51,600 --> 00:06:56,640
+الحاجات اللي كمان بدي اهتم فيها إن هالبني آدم مش
+
+60
+00:06:56,640 --> 00:07:02,140
+عارف إيش بيساوي فبكل بساطة ممكن هو يخبط يبقى نايم
+
+61
+00:07:02,140 --> 00:07:06,120
+على الأرض يخبط رأسه في الأرض ي.. يخبط رأسه يعمله
+
+62
+00:07:06,120 --> 00:07:09,720
+خلل في المخ فبدي أمنعه يخبط في الأرض كيف بده امنعه
+
+63
+00:07:09,720 --> 00:07:13,360
+يخبط الأرض إن
+
+64
+00:07:13,360 --> 00:07:17,140
+هو على السرير بيخبط رأسه في السرير بيحطه على حاجة
+
+65
+00:07:17,140 --> 00:07:22,450
+سفنج على فرشة على مخدة على أي حاجة فبيصير يخبط في
+
+66
+00:07:22,450 --> 00:07:28,270
+إيش؟ في شيء طريق الطريق ألطف من ما.. وهو بخبط هيك
+
+67
+00:07:28,270 --> 00:07:32,430
+كمان في حالة ممكن يكسر حاله بحط بين إيديه مثلا
+
+68
+00:07:32,430 --> 00:07:43,330
+بطانية شرشف كبير اليوم فيه مناشف كبيرة سفنج مخدة
+
+69
+00:07:43,330 --> 00:07:50,500
+أي حاجة بحطها بحيث إنه إيش ما يشدش على نفسه واهم
+
+70
+00:07:50,500 --> 00:07:54,700
+هذه كلها أهم هذه كلها إنه أتأكد إن فيه تنفس ومش
+
+71
+00:07:54,700 --> 00:08:01,140
+طالع لسانه برا وجاصه من شدة اللي هي التشنج اللي
+
+72
+00:08:01,140 --> 00:08:06,060
+بيعمله فبدأ اهتم في من الحاجات اللي أنا مش لازم
+
+73
+00:08:06,060 --> 00:08:10,620
+أعملها مش لازم أقاوم حركات التشنج يعني وهو بيشدّه
+
+74
+00:08:10,620 --> 00:08:15,120
+أشدّه هقول لأ بدناش نجريه اتحرك إذا بدناش لأنه بيبقى
+
+75
+00:08:15,120 --> 00:08:20,230
+قلت لكم عنده قوة يمكن بتوازي قوة عشرة سبحان الله
+
+76
+00:08:20,230 --> 00:08:25,490
+فلو جيت أنت شادّه وأنت تشدي ممكن تعملي له تمزق عضلي
+
+77
+00:08:25,490 --> 00:08:37,350
+ولا تعملي له مشاكل فسيبيه إنما امنعي يؤذي نفسه طبعا
+
+78
+00:08:37,350 --> 00:08:42,250
+إذا توقف التنفس بنعمله تنفس صناعي وينصح إنه إذا
+
+79
+00:08:42,250 --> 00:08:45,210
+بدأنا نعمله تنفس صناعي نعمله تنفس صناعي من الفم
+
+80
+00:08:45,210 --> 00:08:52,010
+للأنف من الفم للأنف ليش من الفم للأنف؟ لإنه بيبقى
+
+81
+00:08:52,010 --> 00:08:57,710
+تِمه مشدد، مسكر على الآخر ومحنّجون بالكز، مش شكر
+
+82
+00:08:57,710 --> 00:09:01,890
+فكيف ده كنت فتحته بموت؟ مش سهل لكن مع أنفه ما فيش
+
+83
+00:09:01,890 --> 00:09:07,890
+إمكانية يتشنج، الأنف لو إيش ما صار بيظل مجرى النفس
+
+84
+00:09:07,890 --> 00:09:17,590
+فاتح فبعمله تنفس عن طريق الأنف مش لازم أرشّ ميه
+
+85
+00:09:17,590 --> 00:09:22,900
+بطريقة عشوائية على جسمه خاصة على الوجه لأنه لو
+
+86
+00:09:22,900 --> 00:09:27,160
+رشيت ميه عشوائي على الوجه وأجت في تِمه أو أجت في
+
+87
+00:09:27,160 --> 00:09:32,380
+القنف ونزلت في الرقبة هيصير جحجح وهيصير مشكلة
+
+88
+00:09:32,380 --> 00:09:36,400
+في التنفس وهيصير مشاكل يعني أنا في غنى عنها فإذا
+
+89
+00:09:36,400 --> 00:09:41,040
+بدي أحط له ميه ولا بد بمسح بقطنة أو بشريط قطعة
+
+90
+00:09:41,040 --> 00:09:46,680
+قماش بمسحه مسح أو بحط بطريقة يعني أعرف وين أحط
+
+91
+00:09:46,680 --> 00:09:52,800
+اللي هي الميه مش الحال في حاجة أخرى بتعمل برضه تشنج
+
+92
+00:09:52,800 --> 00:09:59,000
+اسمها الصرع إيش يعني الصرع الصرع
+
+93
+00:09:59,000 --> 00:10:06,180
+زي وزي التشنج إلا إنه يعني الصرع هذا عبارة عن
+
+94
+00:10:06,180 --> 00:10:10,960
+مرض ما له علاقة بأمراض أخرى يعني مش شرط يكون عنده
+
+95
+00:10:10,960 --> 00:10:16,100
+حرارة أو عنده مثلا هواي في الراس أو أو الآخر زي
+
+96
+00:10:16,100 --> 00:10:21,200
+اللي هو التشنج والكثير من الناس بيقول لك كهرباء المخ
+
+97
+00:10:21,200 --> 00:10:25,520
+بيعملوا له وبتطلع الكهربا عادي بيعملوا له رسم أو
+
+98
+00:10:25,520 --> 00:10:30,520
+تخطيط دماغ وبيطلع الكهربا زيادة في المخ مرت عنكم
+
+99
+00:10:30,520 --> 00:10:35,380
+قصة المرأة اللي جاءت للنبي صلى الله عليه وسلم مين
+
+100
+00:10:35,380 --> 00:10:43,180
+مرت عنها حكيتها إيش فهمنا منها هذه القصة إن
+
+101
+00:10:43,180 --> 00:10:47,670
+الصرع هذا ما فيش اللي دوه النبي قال له النبي قال لها
+
+102
+00:10:47,670 --> 00:10:52,550
+إن شئتي دعوت لك وإن شئتي صبرتي وودّكي الجنة مين
+
+103
+00:10:52,550 --> 00:10:55,370
+عقل بيقول لأ والله ده إيه اللي بدي أطيب وبعد
+
+104
+00:10:55,370 --> 00:10:59,990
+بكرا أموت موتة طبيعية يمكن بكرا يمكن اليوم طب لا والله
+
+105
+00:10:59,990 --> 00:11:04,430
+بدي أكسب الجنة الناس قاعدة بتتقطع لإيش عشان
+
+106
+00:11:04,430 --> 00:11:09,370
+تروح الجنة ولا لأ النبي كان إيش بوعد الناس كان
+
+107
+00:11:09,370 --> 00:11:13,810
+بيقول يعني إنه أُقل كل جنة لدرجة أحد الصحابة رضوان
+
+108
+00:11:13,810 --> 00:11:17,480
+الله عليهم بيقول يعني ليس بيني وبين الجنة إلا أن أكل
+
+109
+00:11:17,480 --> 00:11:22,480
+هذه الثمرات والله إنها لحياتهم طويلة فرمى الثمرات
+
+110
+00:11:22,480 --> 00:11:28,840
+وخش المعركة مرت عنكم يمكن القصة ماشي فيعني كأنه
+
+111
+00:11:28,840 --> 00:11:32,100
+النبي صلى الله عليه وسلم قال لها مرضك ما له علاج
+
+112
+00:11:32,100 --> 00:11:36,060
+وفعلا لليوم الصرع يا أخوات المجدات ما له علاج
+
+113
+00:11:36,060 --> 00:11:43,900
+شافي ما له علاج إيش شافي إنما إله علاج عرضي إيش
+
+114
+00:11:43,900 --> 00:11:50,420
+يعني علاج شافي وعلاج عرضي نعم لأ
+
+115
+00:11:50,420 --> 00:11:56,580
+بيضيع الأعراض أو بيخفف الأعراض لكن أن يشفى يعني
+
+116
+00:11:56,580 --> 00:12:01,780
+مثلا عندنا واحد عنده رشح مثلا بنعالجه للرشح نروح
+
+117
+00:12:01,780 --> 00:12:08,440
+الرشح وبيطيب واحد عنده مثلا التهابات نعطيه علاجات
+
+118
+00:12:08,440 --> 00:12:14,270
+للالتهاب بيطيب لكن الصرع نعطيله علاج عشان هيك هذا
+
+119
+00:12:14,270 --> 00:12:20,750
+بيسموه علاج باقي الأمر علاج ع باقي الأمر يعني لو
+
+120
+00:12:20,750 --> 00:12:24,610
+يوم بطل أو يومين تلاتي بطل ما ياخدش العلاج ممكن
+
+121
+00:12:24,610 --> 00:12:28,470
+تصير الحالة أسوأ عشان هيك دائما لازم يكون عندهم
+
+122
+00:12:28,470 --> 00:12:32,410
+علاج وكنا زمان ننصح الناس اللي بياخدوا العلاج
+
+123
+00:12:32,410 --> 00:12:40,070
+المزمن هذا إنه دائما يحتفظ بشهر علاج في الضغط لأن
+
+124
+00:12:40,070 --> 00:12:43,700
+ممكن تروح على العيادة يقول لك والله خلصوا تكون عندك
+
+125
+00:12:43,700 --> 00:12:47,780
+إيش؟ آخر حبة خدتيها اليوم الصبح وأنت ماشي ما شاء الله 
+
+126
+00:12:47,780 --> 00:12:52,160
+الله على الدور وكل شهر بتروح تجيب علاجك فخلص من
+
+127
+00:12:52,160 --> 00:12:56,040
+العلاج طب مدور في السوق ما لاقيش أو ما فيش معانا 
+
+128
+00:12:56,040 --> 00:13:01,060
+مصاري نشتري فكنا ننصح دائما يكون عندهم علاج احتياط
+
+129
+00:13:01,060 --> 00:13:04,740
+لشهر فاليوم طبعا يعني الناس بتلاقي عندها يعني
+
+130
+00:13:04,740 --> 00:13:09,740
+موجود وبنعني عليه الصرع هذا مرض
+
+131
+00:13:11,080 --> 00:13:17,200
+غالبا بكون فيه زيادة في كهربية المخ وما فيش علاج
+
+132
+00:13:17,200 --> 00:13:24,040
+شافي إنما علاج عرضي بمعنى أنه احنا إيش فيه أعراض
+
+133
+00:13:24,040 --> 00:13:32,420
+بتظهر بنحاول نحد منها أو نوقفها بمعنى لما ألاقي طفل
+
+134
+00:13:32,420 --> 00:13:38,460
+طفل بيتشنج عشرين مرة في اليوم هذا حصل يعني مش
+
+135
+00:13:38,460 --> 00:13:44,000
+ما حصلش، بلغت أنه كان في طفل في المستشفى قالوا هذا
+
+136
+00:13:44,000 --> 00:13:47,400
+مش أقل من عشرين مرة في الـ 24 ساعة بيتشنج
+
+137
+00:13:47,400 --> 00:13:52,440
+هذا لو صار مرة في اليوم يتشنج إيش أهله بيقولوا إيش
+
+138
+00:13:52,440 --> 00:13:58,100
+الحمد لله طار مش هيك بيقولوا لو واحد بيتشنج كل ست
+
+139
+00:13:58,100 --> 00:14:02,700
+شهور صار كل ست سنين بيقولوا احنا وين جينا ووين
+
+140
+00:14:02,700 --> 00:14:08,000
+صرنا كمان لما بيجي تجي النوبة يكون شد شديد جدا وي
+
+141
+00:14:08,000 --> 00:14:12,060
+..ويعني يتعب حاله ويتعب اللي حواليها الحين بتجي
+
+142
+00:14:12,060 --> 00:14:17,840
+أخف شوية ويتحسن فالعلاج اللي موجود اليوم للصرع
+
+143
+00:14:17,840 --> 00:14:26,960
+علاج لباقي العمر بيخفف النوبة وبيبّعد اللي هي إيش
+
+144
+00:14:27,680 --> 00:14:32,400
+الفترة بين النوبة وأختها فيعني ممكن واحد يستفيد
+
+145
+00:14:32,400 --> 00:14:37,560
+من وقته يصير يشتغل شغلات فاحنا هدول الناس كمان
+
+146
+00:14:37,560 --> 00:14:44,060
+يعني بننصحهم أنه يشتغلوا شغلات خاصة إيش يعني شغلات
+
+147
+00:14:44,060 --> 00:14:49,740
+خاصة؟ يعني شغلات ما تعرضش حياته للخطر يعني خطأ جدا
+
+148
+00:14:49,740 --> 00:14:53,600
+أن نشتغل سواق على الخط واخدين بالكوا كيف؟ مين ساكن
+
+149
+00:14:53,600 --> 00:14:58,880
+في جباليا فيكم؟ والواحدة من جباليا من جباليا سمعتي
+
+150
+00:14:58,880 --> 00:15:06,300
+في واحد سواق بقى عنده صرع وخّش في السيارة في حفة
+
+151
+00:15:06,300 --> 00:15:14,520
+الأسفل ومات واتكسرت السيارة في كان واحد عنده صرع
+
+152
+00:15:14,520 --> 00:15:17,900
+وكان بيجيب مجموعة ممرضين أنا بعرف الحادث لأنه صار
+
+153
+00:15:17,900 --> 00:15:22,900
+مع شبابنا يعني بيجيب مجموعة ممرضين من جباليا على
+
+154
+00:15:22,900 --> 00:15:26,830
+مستشفى النصر مستشفى الأطفال فالراجل وهو في الطريق
+
+155
+00:15:26,830 --> 00:15:32,250
+أجته النوبة أجته النوبة شد شد إجره على الكاز كبس
+
+156
+00:15:32,250 --> 00:15:36,910
+على الآخر خبطت منه السيارة اللي هي جاية في منطقة
+
+157
+00:15:36,910 --> 00:15:43,050
+صلبة خشت السيارة في جسمه مات على المطرح والشباب
+
+158
+00:15:43,050 --> 00:15:47,890
+كلهم اللي في السيارة اتعور فمنفعش أخلي واحد مثلا
+
+159
+00:15:47,890 --> 00:15:52,990
+عنده صرع يشتغل يقصر فوق على الخامس والسادس
+
+160
+00:15:52,990 --> 00:15:56,960
+والعاشر بتشوفوهم اللي بيطلعوا على الخشب هذا يقصروا؟
+
+161
+00:15:56,960 --> 00:16:00,420
+ما نفعش برضه واحد عنده صرع يشتغل على منشار حديد
+
+162
+00:16:00,420 --> 00:16:07,440
+أو ماكينة لف منشار لأنه ممكن جدا تجي النوبة ويصير
+
+163
+00:16:07,440 --> 00:16:13,120
+فيه مشكلة زي ما .. يعني يتقطع أيده يتقطع .. يأثر
+
+164
+00:16:13,120 --> 00:16:17,120
+عليه المنشار الحديد فبده هدول الناس ينشغلوا وينشغلوا يعني
+
+165
+00:16:17,120 --> 00:16:19,980
+أنا بعجبني واحد في حد فيكم من ال
+
+166
+00:16:19,980 --> 00:16:24,830
+bridge أو من صراط من الصراط بتشوفوا الشاب اللي بيبقى
+
+167
+00:16:24,830 --> 00:16:30,930
+بيوزع ترمس ومعه خرزانات وعلى كرة بيكون على مدور
+
+168
+00:16:30,930 --> 00:16:35,030
+إنصارات غالبا وبيجي في جوا في إنصارات هذا الشاب
+
+169
+00:16:35,030 --> 00:16:38,950
+أكثر من مرة ألاقيه مدد في الأسفل جايينه وبيتصرع
+
+170
+00:16:38,950 --> 00:16:46,570
+ف بفهم هذا الشاب عنده صرع فبيشتغلش إلا شغل خفيفة
+
+171
+00:16:46,570 --> 00:16:52,030
+شغل الـ termos على الكرة مع الخرزانة مع اللوز مع
+
+172
+00:16:52,030 --> 00:16:57,640
+الأبصار الشوّامة ما بدهاش يعني ما فيش منها خطورة فالراجل
+
+173
+00:16:57,640 --> 00:17:04,260
+بيتمدد وبعد ما يخلص بصحى وبيقوم و kart جنبه تمام
+
+174
+00:17:04,260 --> 00:17:08,700
+تمام وسبحان الله العظيم يا أخوات المجدّات الناس
+
+175
+00:17:08,700 --> 00:17:12,560
+اللي عندهم صرع الله سبحانه وتعالى بيعطيله إشارة
+
+176
+00:17:12,560 --> 00:17:17,760
+أنه كمان شوية هيجيك الصرع بتلاقي عنده علامة
+
+177
+00:17:17,760 --> 00:17:22,840
+بتلاقي مثلا حس إحساس معين اسأل أي واحد عنده صرع
+
+178
+00:17:22,840 --> 00:17:26,360
+بيقول لك فعلا أنا قبل ما تجيني دقيقة أو دقيقتين بعرف
+
+179
+00:17:26,360 --> 00:17:33,580
+أنها بدأت تجيني فبعض الناس بيحتاط خلاص بيتمدد وبينام
+
+180
+00:17:33,580 --> 00:17:38,380
+أو بيحط حاله بعيد عن الخطر كثير عدد شاب أنا أشوفه
+
+181
+00:17:38,380 --> 00:17:43,960
+يعني متشنج يكون فعلا جاب كارته بالظبط من الأسفلت
+
+182
+00:17:43,960 --> 00:17:49,220
+وجوه يعني بعيد عن منطقة الخطر ويعني الولد بيشتغل
+
+183
+00:17:49,220 --> 00:17:54,400
+كويس صحيح فالقصة وما فيها أنه مثلا في صبية في
+
+184
+00:17:54,400 --> 00:17:58,540
+جارتنا جارتنا طبعا أنتو عارفين يعني أن جارتنا أي
+
+185
+00:17:58,540 --> 00:18:04,300
+مشكلة عندهم بكون أنا مارجعيتهم يعني، مظبوط؟ بفقد
+
+186
+00:18:04,300 --> 00:18:08,340
+جارتنا وين فلان قالوا أنت ما معكش خبر؟ خير قال
+
+187
+00:18:08,340 --> 00:18:13,580
+والله فلانة يعني وقعت فوق طنجرة الخبز وانحرجت سدرة
+
+188
+00:18:13,580 --> 00:18:21,400
+ونايمة في الحروق قولوا غيره وقال اهف جارتي وبتخصنا
+
+189
+00:18:21,400 --> 00:18:25,040
+سحبنا حالنا وروحنا نزورها إيش يا أم فلان ليش تسوي
+
+190
+00:18:25,040 --> 00:18:29,060
+في حالك كذا قالت هو بيدي طبعا فلانة هذه معها صرعة
+
+191
+00:18:29,060 --> 00:18:35,580
+فهي تشتغل تخبز قاعدة بتخبز على طنجرة الخبز أجتها
+
+192
+00:18:35,580 --> 00:18:42,180
+النوبة نامت فوق الطنجرة نامت فوق الطنجرة أجى حد
+
+193
+00:18:42,180 --> 00:18:46,730
+قامها وسدرها كله محروق فبقول لها يا ابنت الحلال
+
+194
+00:18:46,730 --> 00:18:49,250
+ليش اللي عملتي في حالك؟ جات هو بيدك؟ قالت لها بيدك
+
+195
+00:18:49,250 --> 00:18:54,890
+يعني أنا بدي أخبز، بدي أقعد لولد صغير جنبي أو خلي
+
+196
+00:18:54,890 --> 00:18:58,670
+حد من الدار يخبز، غيري قالت لها لأ ما جيش أنا
+
+197
+00:18:58,670 --> 00:19:02,350
+أقعد، طبعا هي إيش اللي صار أجتها الإشارة فقالت لي
+
+198
+00:19:02,350 --> 00:19:05,590
+الريف قد انحرجي يلا نص دقيقة وبجيبوها ما جت
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+199
+00:19:05,590 --> 00:19:09,430
+استناش الـ الوجد نص دقيقة اللي هي نايمة، واخدين
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+200
+00:19:09,430 --> 00:19:13,800
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+
+201
+00:19:13,800 --> 00:19:19,960
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+202
+00:19:19,960 --> 00:19:25,340
+أو يأذي الآخرين امنعه أنه يقطع لسانه أخلي طريقة
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+203
+00:19:25,340 --> 00:19:30,120
+التنفس فاتحة واتأكد من أنه بيتنفس ووضعه كويس و
+
+204
+00:19:30,120 --> 00:19:34,800
+بعد ما يصحى من النوبة بينام نوم عميق بقعد له قدت
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+205
+00:19:34,800 --> 00:19:38,600
+ساعات نايم مش داري عن حاله ما جيش أقص له الجثة والله
+
+206
+00:19:38,600 --> 00:19:42,530
+أنت صار معك واتشنجت و.. ما.. ما فيش داعي يعني
+
+207
+00:19:42,530 --> 00:19:47,550
+إيش هيستفيد إيه؟ إنما هأدايقه نفسيا واتعبه وي
+
+208
+00:19:47,550 --> 00:19:54,210
+يصير الوضع مش هو ماشي؟ في حاجة مش واضحة يا صبايا؟
+
+209
+00:19:54,210 --> 00:19:57,810
+في شيء بده إعادة؟ طيب الحين بدنا نخش على حاجة
+
+210
+00:19:57,810 --> 00:20:04,570
+اسمها الغيار والأربطة، ماشي؟ كل كده بتعمل غيار
+
+211
+00:20:04,570 --> 00:20:10,270
+ولا ما بتعرف إنك تعمل غيار إيه؟ أمور كده مش عملت
+
+212
+00:20:10,270 --> 00:20:14,730
+أنت أنت عملت غيار على جسمك بالمرة؟ طب إيش إيش
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+213
+00:20:14,730 --> 00:20:22,790
+الغيّار؟ هو الغطاء الذي يوضع على الجروح مباشرة
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+214
+00:20:22,790 --> 00:20:29,710
+الغيّار كل غطاء أو شيء يوضع على الجروح مباشرة لو
+
+215
+00:20:29,710 --> 00:20:35,650
+قلنا الغيّار هو الغطاء الواقي الذي يوضع فوق الجروح
+
+216
+00:20:35,650 --> 00:20:41,670
+بينحط قبل ما يصير ولا بعد؟ بعد لو جبل إيش معناه
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+217
+00:20:41,670 --> 00:20:46,910
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+218
+00:20:46,910 --> 00:20:51,130
+يثرأ يعني ممكن ما تنجرحش في الجسم فإذا بدنا نحط
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+219
+00:20:51,130 --> 00:20:55,330
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+220
+00:20:55,330 --> 00:21:00,030
+لفين فلا يوضع الغيّار إلا بعد حدوثه طبعا والغيّار
+
+221
+00:21:00,030 --> 00:21:04,970
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+222
+00:21:04,970 --> 00:21:14,090
+الأصل لكن إذا ما فيش غيار معقم بدي أحاول إيش أستخدم ما
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+223
+00:21:14,090 --> 00:21:20,070
+يمكن استخدامه أو أنا أعمل شيء بصد عن هذا الكلام
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+224
+00:21:20,070 --> 00:21:25,730
+فممكن أني أجدى على الميكروبات في الغيارة بقدر
+
+225
+00:21:25,730 --> 00:21:30,970
+الطريقة كيف يعني ممكن أحصل أنا غيار معقم من اللي
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+226
+00:21:30,970 --> 00:21:38,410
+حواليا كيف ممكن أحصل غيار معقم من حوالينا لو
+
+227
+00:21:38,410 --> 00:21:45,540
+أنت في الدار نعم هذا
+
+228
+00:21:45,540 --> 00:21:51,480
+يعتبر غيار معقّر شايفاتوا؟ أيوة مكتوب ومكتوب
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+229
+00:21:51,480 --> 00:21:57,800
+عليها معقّر هذا اسمه غيار معقّر بالظبط
+
+230
+00:21:57,800 --> 00:22:01,280
+لو كانت الملابس على الحبل مغسولة بتغسلوا بإيش
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+231
+00:22:01,280 --> 00:22:08,560
+أنتم؟ مواد مطهرة ومواد يعني منظفة وبتحط في الشمس
+
+232
+00:22:09,580 --> 00:22:14,360
+إيش الشمس بتساوي؟ تقتل البكتيريا فالغسيل بالمواد
+
+233
+00:22:14,360 --> 00:22:22,740
+المطهرة مع حرارة الشمس وأشعة الشمس بيضيع اللي هو
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+234
+00:22:22,740 --> 00:22:27,440
+البكتيريا ففي سيرة غيار ممكن نستخدمه فاللي بيكون
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+235
+00:22:27,440 --> 00:22:30,180
+على الحبل مغسول يده ساعتين تلاتة ممكن نستخدمه طب
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+236
+00:22:30,180 --> 00:22:33,720
+امبارح يا أسد أنت غسلتي وصفطي وحطيتي في الخزانة مش
+
+237
+00:22:33,720 --> 00:22:39,810
+ممكن نظيف؟ ممكن استخدامه؟ طب وإيش كمان ممكن؟ المكوى
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+238
+00:22:39,810 --> 00:22:45,910
+المكوى عبارة عن إيش عبارة عن نار احنا مش قلنا من
+
+239
+00:22:45,910 --> 00:22:50,390
+وسائل التعقيم الحرارة الجافة الحرارة الجافة اللي
+
+240
+00:22:50,390 --> 00:22:57,290
+هي النار المكوى إيش باطي جبنا قطعة قماش ومشينا
+
+241
+00:22:57,290 --> 00:23:02,090
+عليها المكوى أو دقيقة أو دقيقتين إيش بيصير فيها
+
+242
+00:23:02,090 --> 00:23:08,010
+بيصير معقّم ولا لأ إيش كمان ممكن يعملي غيارة نظيفة
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+243
+00:23:09,320 --> 00:23:15,940
+من البيت عندك بولدين عندك كحول عندك أي حاجة لو
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+244
+00:23:15,940 --> 00:23:21,660
+وضعتي الشاشة في جنية الكحول أو في صحن وفي كحول أو
+
+245
+00:23:21,660 --> 00:23:25,760
+في بولدين واستنيت عليها خمس دقايق شو بيصير فيه؟
+
+246
+00:23:25,760 --> 00:23:31,320
+بيصير معقّم ففي أداة حاجات يعني ممكن نستخدمها بتعمل
+
+247
+00:23:31,320 --> 00:23:37,440
+اللي هي لو خطناها في غلاية ومي وغلناها
+
+248
+00:23:44,190 --> 00:23:51,170
+لكن لو كنا في طريق مثلا ممكن نستخدم المناديل ممكن
+
+249
+00:23:51,170 --> 00:23:55,950
+نستخدم فوطة ممكن نستخدم ملابس الإنسان كل هذه
+
+250
+00:23:55,950 --> 00:24:02,270
+الشغلات ممكن نستخدمها مضطرين لها لأنه يعني بحتاج 
+
+251
+00:24:02,270 --> 00:24:07,790
+إلى غطاء للجروح
+
+252
+00:24:08,770 --> 00:24:14,270
+طب احنا ليش بنعمل غيار يا صغير؟ أول حاجة مجرد ما
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+253
+00:24:14,270 --> 00:24:20,150
+تنجرحي بتغيري ليش بتغيري؟ توقف النظيف فالغيار من 
+
+254
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+شغلته أنه بوقف النظيف الحاجة الثانية اللي غيار إيش
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+255
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+بعمل؟ الغيار بيمتص الإفرازات اللي بيفرزها الجرح
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+256
+00:24:33,310 --> 00:24:39,960
+كان صديد، كان دم، كان إفرازات كلها هذه بيمتصها
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+257
+00:24:39,960 --> 00:24:46,480
+ويمتصها معناته إيش؟ معناته صار ما فيش زيادة رطوبة جوا
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+258
+00:24:46,480 --> 00:24:51,760
+وما فيش نمو بكتيريا زيادة فبيساعد على إيه؟ على التئام
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+259
+00:24:51,760 --> 00:24:58,960
+فأول حاجة امتصاص الإفراز وهذا بحد ذاته بيؤدي إلى إيش؟
+
+260
+00:24:58,960 --> 00:25:03,600
+المساعدة على الشفاء يبقى كمان من فوائد الغيار إيش؟
+
+261
+00:25:03,600 --> 00:25:08,300
+مساعدة على سرعة الالتئام تعالي أنتِ ما بتغيريش على جرح 
+
+262
+00:25:08,300 --> 00:25:14,240
+معين بطول لكن غيري عليه ثلاثة أربعة أيام بتلاقيه
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+263
+00:25:14,240 --> 00:25:21,960
+بإيش؟ بسهولة بطيب كمان إيش من فوائد الغيار بمنع
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+264
+00:25:21,960 --> 00:25:27,840
+الميكروبات اللي في الجو أنتِ بتحطي فاصل بين الجسم و
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+265
+00:25:27,840 --> 00:25:32,500
+بين الميكروبات فبيصير يمنع الميكروبات وبيقتل
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+266
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+الميكروبات إذا كان حطيتي دواء معينة أو شيء معين ممكن
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+267
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+يقتل الـ microbe إيش كمان ممكن يعمل الغيار حماية
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+268
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+الجسم من المؤثرات الخارجية إيش يعني؟ صح كلامك إيش
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+269
+00:25:48,070 --> 00:25:53,350
+يعني؟ نعم
+
+270
+00:25:53,350 --> 00:26:00,350
+ممكن أسألك سؤال عندكم حمامات على الكهرباء في الدار؟
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+271
+00:26:00,350 --> 00:26:04,510
+آه عمرك حسيتي فيه ماس؟
+
+272
+00:26:07,520 --> 00:26:14,640
+الماء الماء فيها ماس؟ ماس نعم؟
+
+273
+00:26:14,640 --> 00:26:22,040
+مين عمرها حست الكهرباء ماس في الماء؟ نعم؟ حسيتي ماس
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+274
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+في الماء؟ بتيجي بتقولي الكهرباء في في الحمام ماس بيجي
+
+275
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+أبوك أخوك أختك أمك بتمسك بتقولك يا عم ما فيش ماء 
+
+276
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+بتطلعي في يدك بتلاقي يدك مجروحة هذه حالة 100% صح ولا
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+277
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+278
+00:26:45,350 --> 00:26:53,890
+أخوك ما حسش أنه ماس معناته الجرح... الجرح خلى
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+279
+00:26:53,890 --> 00:26:59,210
+الأعصاب تبان ربنا بيقول سبحانه وتعالى عن الناس
+
+280
+00:26:59,210 --> 00:27:05,190
+اللي في النار إيش؟ كلما نضجت جلودهم بدلناهم جلودا
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+281
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+غيرها ليش؟ يبقى الأعصاب وين؟ تحت الجلد لما نفتح
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+282
+00:27:11,850 --> 00:27:16,370
+الجلد هذا الجلد مغطى وين؟ على أعصاب فبنحميها من
+
+283
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+المؤثرات الخارجية مجرد ما تبان هذه الأعصاب شو
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+284
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+بيصير؟ بيصير تتأثر بالمؤثرات الخارجية أعجبتك؟ كده
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+285
+00:27:26,210 --> 00:27:31,130
+اتجاه بعدك هيك؟
+
+286
+00:27:31,130 --> 00:27:35,700
+هي هيك بالظبط فحماية الجسم من المؤثرات الخارجية
+
+287
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+المهيجة للأعصاب وبالتالي بتخف الألم عشان هيك بيبقى
+
+288
+00:27:40,400 --> 00:27:45,680
+يدك توجعك أو الجرح يوجعك بتغطيه بيبطّي الوجع بتمنع 
+
+289
+00:27:45,680 --> 00:27:53,600
+عنه المؤثرات الخارجية هذا
+
+290
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+291
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+الغيار عرفنا طب كيف بنستخدم الغيار الغيار
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+292
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+على حسب الجرح والله جرح صغير احنا قاعدين بنحكي
+
+293
+00:28:10,640 --> 00:28:17,080
+إسعاف أولي إسعاف أولي يعني إصابات كبيرة ماشي إسعاف
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+294
+00:28:17,080 --> 00:28:22,680
+أولي إصابات كبيرة لازم ينحط الغيار على الجرح ويكون
+
+295
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+زيادة عن الجرح يعني ما يكونش بالظبط على الطرف لأن
+
+296
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+
+297
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+معناته ما غطيناش معناته ما بنعملش اللازم يبقى صار
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+298
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+يخش التلوث وصار تخش البكتيريا كمان من الحاجات
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+299
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+المهمة في الغيار أنه بدنا نحاول نخلي الأطراف
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+300
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+مكشوفة إذا قدرنا يعني أصابع اليدين وأصابع
+
+301
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+الرجلين بدي أحاول أغطّيهاش ليش؟ لأنه
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+302
+00:29:02,950 --> 00:29:08,710
+في حاجتين الحاجة الأولى إن الأطراف هذه لو انضغطت
+
+303
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+
+304
+00:29:15,010 --> 00:29:19,710
+احنا عندنا في المستشفى لما نركب جبس لواحد على
+
+305
+00:29:19,710 --> 00:29:26,050
+يده أو على رجله بنخلي أصابع مبينات ليش؟ عشان
+
+306
+00:29:26,050 --> 00:29:31,850
+أتابع الدورة الدموية وأتابع الجهاز العصبي يعني
+
+307
+00:29:31,850 --> 00:29:38,950
+اللي هي الإحساس ودورة دموية إن صار ازرقاق صار ورم
+
+308
+00:29:38,950 --> 00:29:46,970
+في الأطراف معناته في إيه ضغط بزيادة فبنفك إن صار
+
+309
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+تنميل تنميل زي ما في نمل كأنه نمل بيمشي على الأطراف
+
+310
+00:29:50,670 --> 00:29:55,410
+معناته مضغوط العصب فأنا كيف بدي أعمل هذه الشغلة
+
+311
+00:29:55,410 --> 00:30:00,600
+إذا كان بدي أخلي أطراف الإصابة أو أطراف ال... ايدين
+
+312
+00:30:00,600 --> 00:30:07,120
+ورجل مسكرات ما بظبطش كمان الحاجات الثانية أنه ال...
+
+313
+00:30:07,120 --> 00:30:15,160
+الأيدي الأذرع إن بعض الناس لو لفيتيها ممكن تأثر
+
+314
+00:30:15,160 --> 00:30:18,460
+على الدورة الدموية يعني كان في يوم أنا روحت على
+
+315
+00:30:18,460 --> 00:30:23,520
+مستشفى الشفاء وكان في مشرف ومعه طلاب وكان في واحد
+
+316
+00:30:23,520 --> 00:30:27,640
+نام في المستشفى يا صبايا يعني سبحان الله العظيم
+
+317
+00:30:27,640 --> 00:30:35,340
+بني آدم مسكين عنده سكري فراح على الجراح مرضيش
+
+318
+00:30:35,340 --> 00:30:40,060
+فهيئانه يجيب له إصبعه راح على الجراح جيب له نص الرجل 
+
+319
+00:30:40,060 --> 00:30:45,240
+طبعا ونص الرجل وقالوا له بدنا نجيب لك ثاني فجيب له
+
+320
+00:30:45,240 --> 00:30:52,680
+على الشفاء جيب له على الشفاء بدنا أطراف يديه و
+
+321
+00:30:52,680 --> 00:30:58,150
+رجليه يزرع غير اللي نقص يعني كله بيصير نقص
+
+322
+00:30:58,150 --> 00:31:02,510
+فبطلوا طبعا يقصلوا يعني فجيت جيت الشباب بيغيروا
+
+323
+00:31:02,510 --> 00:31:07,930
+للزلمة لفّين على رجله مسكت المشرف تبعه وقلت له يا
+
+324
+00:31:07,930 --> 00:31:11,430
+أخويا الله يرضى عليك شايف أنت هذا الزلمة إيش
+
+325
+00:31:11,430 --> 00:31:16,990
+مشكلته؟ إيش مشكلته؟ أنه عنده مشكلة في وصول الدم
+
+326
+00:31:16,990 --> 00:31:21,690
+لجسمه لأطرافه فتروح تحط غيار وتلفه إيش معناته؟
+
+327
+00:31:22,350 --> 00:31:27,630
+زودت مشكلة وصول الدم قال لي والله كلامك صح فجيبنا
+
+328
+00:31:27,630 --> 00:31:33,430
+الغيار جيبنا الفاف وحطينا لزيّج حطينا إبداع أسطر
+
+329
+00:31:33,430 --> 00:31:41,630
+خاص عشان ما يعملش الضغط فلازم
+
+330
+00:31:41,630 --> 00:31:46,850
+ما يتغطّش على القوية الدموية وممكن نثبته بس بمادة
+
+331
+00:31:46,850 --> 00:31:54,600
+لاصقة عشان ما يخشش في مشاكل ماشي؟ وأصابعك دي ازرقت؟
+
+332
+00:31:54,600 --> 00:31:58,300
+والله مسكين يعني بدهم يجسسوا له كله ما فيش و مات يعني
+
+333
+00:31:58,300 --> 00:32:02,580
+جسّوا له رجله وبعدين طلع ولاجوا رجله الثانية ازرقت
+
+334
+00:32:02,580 --> 00:32:07,660
+وطلع أصابع دي ازرقت وكل ما يجسسوا له بيمشي يعني ف...
+
+335
+00:32:07,660 --> 00:32:11,880
+مسكين يعني خلاص ما فيش أمل يعني والله لما تيجي
+
+336
+00:32:11,880 --> 00:32:17,960
+لوحد مريض بيخلص ويقول لك بالله يعني أهل سألوكِ إيش
+
+337
+00:32:17,960 --> 00:32:22,680
+انطعميه؟ إيش بتطعميه؟ إيه اللي بدوا ياه؟ أنت يا زنا
+
+338
+00:32:22,680 --> 00:32:23,100
+مخربت الدنيا أنت عملت أنت... ليش؟ لأن الابن طعمي
+
+339
+00:32:23,100 --> 00:32:28,000
+إيش؟ ليش اللي بدو ياه؟ لأن ما فيش فيه أمل، لكن لو
+
+340
+00:32:28,000 --> 00:32:34,740
+واحد يعني مريض وجوي وشباب ويعني ممكن يعني يطيب
+
+341
+00:32:34,740 --> 00:32:40,800
+مئة في المئة ونخلف الأكل ندفع، بننجنب، أنت يا زنا
+
+342
+00:32:40,800 --> 00:32:42,820
+مخربت الدنيا، أنت عملت...
+
+343
+00:32:53,770 --> 00:32:57,430
+من دون جرح... من دون جرح لو إياه دموعية بيسكر و
+
+344
+00:32:57,430 --> 00:33:06,590
+بنشر والله أيام يمكن... يمكن في الستينات بس راجل
+
+345
+00:33:06,590 --> 00:33:12,130
+ناصح وعنده سكري أنا بعرفه يمكن من أربعين سنة و
+
+346
+00:33:12,130 --> 00:33:16,270
+هذا الراجل اللي يعني... اللي علاقة معه كان مش 
+
+347
+00:33:16,270 --> 00:33:22,060
+منضبط بالمرض فكنا لما نقعد في فرح مثلا في الفرح
+
+348
+00:33:22,060 --> 00:33:26,160
+بيجيبوا المحليات طبعا هو عارف إن أنا بقول له ما تصبش
+
+349
+00:33:26,160 --> 00:33:31,840
+فقال إيش يساوي مسكين الله يرحمني يعني ما عليش وال...
+
+350
+00:33:31,840 --> 00:33:37,100
+...الواحد ال...الجاجر نفرت عجاة رأسها عفرت مش هيك
+
+351
+00:33:37,100 --> 00:33:41,780
+يجي قال يمسك شجفة المحليات طرف صوابه زي هيك هي و
+
+352
+00:33:41,780 --> 00:33:45,560
+يطلع فيها ويلجمها تلجيم يعني إنه ما أصابهاش يدوموا
+
+353
+00:33:46,400 --> 00:33:50,400
+احنا كيف خشينا الحلو ولا إنه دخلنا حلو كم ما 
+
+354
+00:33:50,400 --> 00:33:55,520
+يدخل الحلو في جسمك يزود السكر ولا مش هي طبعا راجل
+
+355
+00:33:55,520 --> 00:34:00,040
+كبير وواعي وبيفهم ولو فتحت معه موضوع بتسلكيش
+
+356
+00:34:00,040 --> 00:34:07,640
+معه من الحكيم خلاص وبده يأكل حلو فالله يلطف فيه
+
+357
+00:34:07,640 --> 00:34:10,580
+الأربطة إيش يعني أربطة
+
+358
+00:34:14,450 --> 00:34:19,470
+الرباط يا أخوات المجددات أي حاجة بتلصق للتثبيت
+
+359
+00:34:19,470 --> 00:34:24,690
+عبارة عن أشرطة من القماش أو ما شابه ولا يشترط أن
+
+360
+00:34:24,690 --> 00:34:30,950
+تكون معقمة وتستخدم للآثار إيش...إيش فايدة للرباط؟
+
+361
+00:34:30,950 --> 00:34:36,850
+ليش بنستخدم الرباط؟ بمنع الكسر؟
+
+362
+00:34:36,850 --> 00:34:41,130
+الحين أنا لو حطيت رباط يدي ما بنكسرش؟
+
+363
+00:34:43,490 --> 00:34:48,190
+بثبت الكسر قصدك ما تقول تثبيت الكسر لو واحد مكسور
+
+364
+00:34:48,190 --> 00:34:54,210
+بدنا نحط جبيرة كيف بدنا نثبتها الجبيرة؟ بدها رباط
+
+365
+00:34:54,210 --> 00:34:59,390
+كمان لو حطينا... لو أنتِ جيت لك على العيادة مثلا
+
+366
+00:34:59,390 --> 00:35:05,770
+وحطيت لك قطنة وشاش وكذا ولفّتها وظبطت هيك وما حطيتش رباط
+
+367
+00:35:05,770 --> 00:35:11,470
+وقلت لك اقومي بترضي تقومي؟ ما بقومش ليش ما بقومش؟ لأنه
+
+368
+00:35:11,470 --> 00:35:14,750
+نوصلت الباب بالديجة يا المفضل طال طول اليوم وأنا
+
+369
+00:35:14,750 --> 00:35:19,850
+ماسك إيه زيّه تثبيت الغيار الأربطة برضه تثبيت
+
+370
+00:35:19,850 --> 00:35:27,690
+الغيار إيش كمان ذاكرات لما قلنا عدة الثعبان إيش
+
+371
+00:35:27,690 --> 00:35:34,630
+سوينا فيها ربطناها استخدمنا الرباط لمنع سريان السم
+
+372
+00:35:34,630 --> 00:35:40,660
+طب والنزيف استخدمنا في البت... استخدمنا في البت
+
+373
+00:35:40,660 --> 00:35:46,540
+الرباط ضغط فممكن نستخدم رباط ضغط كمان لوقف اللي هو
+
+374
+00:35:46,540 --> 00:35:54,260
+ما يسمى بالنزيف خاصة في الجرح البثري طبعا أربطة
+
+375
+00:35:54,260 --> 00:36:00,320
+تثبيت أجزاء الجسم هناك عدة أنواع وأشكال من 
+
+376
+00:36:00,320 --> 00:36:04,580
+الأربطة أخواتي المجدات من وين بدي أجيب رباط أنا في
+
+377
+00:36:04,580 --> 00:36:07,240
+الـ .. في الإسعاف
+
+378
+00:36:09,650 --> 00:36:16,130
+يعني أنا في .. في منطقة في مثلا أشط البحر ومش بنين
+
+379
+00:36:16,130 --> 00:36:21,850
+صيدلية أشط البحر من وين أجيب البحر؟ أي حاجة حواليا
+
+380
+00:36:21,850 --> 00:36:26,450
+ممكن أستخدمها  في مثلا لو سألتك سؤال مين عمرها
+
+381
+00:36:26,450 --> 00:36:36,630
+شرت ملخي من السوق؟ ملخي من السوق تشتريه؟ مين عمرها
+
+382
+00:36:36,630 --> 00:36:40,620
+شرت؟ طب مين عمرها شرفت أمها لما جابت الملوخية من
+
+383
+00:36:40,620 --> 00:36:49,080
+السوق؟ كيف بتبقى مربوطة؟ بتبقى مربوطة بـ .. بـ .. بـ
+
+384
+00:36:49,080 --> 00:36:54,620
+.. بخيط أسود و أحيانا تبقى مربوطة بعودي ملوخية من
+
+385
+00:36:54,620 --> 00:37:00,260
+نفس العودي و أحيانا تبقى مربوطة بسعف النخل اللي في
+
+386
+00:37:00,260 --> 00:37:05,960
+جريدة النخل هذه كلها أرباط ولا مش أرباط؟ أرباط
+
+387
+00:37:05,960 --> 00:37:11,280
+بيثبت يعني اللي بدي أوصله بالظبط أنه ممكن أجيب أرباط
+
+388
+00:37:11,280 --> 00:37:15,960
+من أي حاجة حواليا يعني لو جيت على شجرة طرية و
+
+389
+00:37:15,960 --> 00:37:22,020
+قصيتي منها شيء طري ممكن تستخدمي رباط أنا في الدار
+
+390
+00:37:22,020 --> 00:37:27,320
+ممكن أستخدم سلك رباط أحيانا في الشارع لو انزنقت
+
+391
+00:37:27,320 --> 00:37:30,720
+ممكن أستخدم السلك اللي بيبقى مرمي في الشارع رباط
+
+392
+00:37:30,720 --> 00:37:35,660
+أي حاجة بتنفعني أثبت الشيء اللي بدي أثبته تعتبر
+
+393
+00:37:35,660 --> 00:37:43,540
+إيش رباط الأربطة بشكل عام يعني هذا عبارة عن أرباط
+
+394
+00:37:43,540 --> 00:37:50,820
+اسمه رباط إيش؟ مشد أو رباط ضغط، مظبوط؟ هذا مشد
+
+395
+00:37:50,820 --> 00:37:55,580
+أو رباط ضغط في مشد في أربطة اسمها أربطة شاش
+
+396
+00:37:55,580 --> 00:38:01,420
+عرفتوا رباط الشاش؟ رباط الشاش هذا عبارة عن عدة
+
+397
+00:38:01,420 --> 00:38:11,110
+أحجام في اتنين ونصف وفي 12 سانتي فيه 2.5 سانتي
+
+398
+00:38:11,110 --> 00:38:16,110
+وفيه 12 سانتي وكل واحد له استخداماته يعني أنا
+
+399
+00:38:16,110 --> 00:38:22,230
+بعرفش أربط بـ 2.5 سانتي الفخد واحد فخده بدي أحط
+
+400
+00:38:22,230 --> 00:38:26,350
+عليه غيار بقى أربط بـ 2.5 سانتي أحطله على فخدته
+
+401
+00:38:26,350 --> 00:38:32,350
+وكمان بأجيش عليه أبوه 12 سانتي أربط إصبع أنت ماخد
+
+402
+00:38:32,350 --> 00:38:38,690
+مالك زي ما بقوله ما يكفي يكفي ما يكفي يكفي هذا
+
+403
+00:38:38,690 --> 00:38:43,470
+بيكفي أو اتنين ونصف أو اتنين سانتي ولا لأ
+
+404
+00:38:43,470 --> 00:38:48,330
+فبمعنى في عندي من اتنين ونصف إلى اتناشر سانتي
+
+405
+00:38:48,330 --> 00:38:51,990
+اليوم مش بس سبعة ونصف في أرض بطاقة اليوم
+
+406
+00:38:51,990 --> 00:38:57,750
+طولها حوالي اتناشر سانتي في زي ما قلنا اللي هو
+
+407
+00:38:57,750 --> 00:39:03,950
+لرباط الضغط اللي هو عبارة عن مشد هذا لرباط الضغط
+
+408
+00:39:03,950 --> 00:39:12,470
+إيش مواصفاته؟ مواصفاته أنه فيه مغيط وبيدي يعني ضغط
+
+409
+00:39:12,470 --> 00:39:18,010
+متزن وكويس ومن ميزاته .. من ميزاته أنه فيه مغيط
+
+410
+00:39:18,010 --> 00:39:25,190
+كمان من عيوبه أنه إيش .. فيه مغيط واخدين بالكم؟
+
+411
+00:39:25,190 --> 00:39:30,050
+كيف؟ من ميزاته أنه فيه مغيط ومن عيوبه أنه فيه مغيط
+
+412
+00:39:30,050 --> 00:39:35,490
+طب كيف هالحكي هذا؟ بركب؟ طب خلينا نقول مثلا من
+
+413
+00:39:35,490 --> 00:39:42,070
+ميزات السيارة أنها بتمشي بسرعة ومن ميزات السيارة
+
+414
+00:39:42,070 --> 00:39:47,530
+الممتازة أنها بتوقف بسرعة ولا لأ؟ يعني تصوري سيارة
+
+415
+00:39:47,530 --> 00:39:52,230
+بتمشي 120 كيلو في الساعة وما لهاش break برضه ..
+
+416
+00:39:52,230 --> 00:39:55,930
+برضه حد يركبها؟ طب بدنا نوقفها طب ما هتخبط في
+
+417
+00:39:55,930 --> 00:40:01,310
+حيطة، هتخبط في سيارة، هتخبط فينا أدمين فأحيانا
+
+418
+00:40:01,310 --> 00:40:09,380
+الحاجة بتكون ميزة وعيب الآن في السرعة إذا ما فيش
+
+419
+00:40:09,380 --> 00:40:14,120
+break مشكلة وإذا ما بترضاش تسرع وأنا عايزها تمشي
+
+420
+00:40:14,120 --> 00:40:18,840
+مشكلة برضه ولا لأ أنا بدي أمشي بدي أسرع تصوري أنت
+
+421
+00:40:18,840 --> 00:40:24,380
+سيارة جيت فيها من دارك لها من سرعة لغزة أخدت منك
+
+422
+00:40:24,380 --> 00:40:29,820
+ساعتين ونص وهي ماشية درجة درجة كأنه واحد بزجها زج
+
+423
+00:40:29,820 --> 00:40:38,290
+مشكلة فعيب هذا أنه في مغيط ميزة أنه فيه مغيط ميزة
+
+424
+00:40:38,290 --> 00:40:44,590
+أنه فيه مغيط بيديني ضغط متزن وبيديني دعم للمنطقة
+
+425
+00:40:44,590 --> 00:40:51,550
+المصابة عيبه أنه فيه مغيط أنه ممكن يحزج زيادة
+
+426
+00:40:51,550 --> 00:40:59,070
+ويمنع وصول الدم ويأثر على الأصابع واخدين بالكم كير
+
+427
+00:40:59,070 --> 00:41:06,840
+وهذه برضه من عيوبه في عندنا إشارة رباط المثلث رباط
+
+428
+00:41:06,840 --> 00:41:11,100
+المثلث عارفين من وين بجيبه؟ في واحد معاه منديل
+
+429
+00:41:11,100 --> 00:41:16,520
+خربان أنا غالبا غالبا نسيت أجيب منديل يعني بجيب
+
+430
+00:41:16,520 --> 00:41:24,160
+معاه منديل منديل أو قماش منديل نعم بالظبط
+
+431
+00:41:24,160 --> 00:41:28,220
+أربطة المثلث عبارة عن منديل جيب المنديل اللي بدك
+
+432
+00:41:28,220 --> 00:41:33,880
+ترميه وما ترضيش تلبسه لأنه اتنتش أو انمزع أو اعتجو
+
+433
+00:41:33,880 --> 00:41:37,820
+اتنى اتنى بيصير منديل رباط مثلث ولو جثثته اتنى
+
+434
+00:41:37,820 --> 00:41:42,600
+بيصير عندك منديل رباطين مثلث طبعا هذا الرباط
+
+435
+00:41:42,600 --> 00:41:48,520
+المثلث له عدة استخدامات ويعني له أشكال معينة طبعا
+
+436
+00:41:48,520 --> 00:41:54,440
+لازم يكون طوله حوالي متر الظلق هذا رباط هذا مش
+
+437
+00:41:54,440 --> 00:42:00,960
+مثلث على رفيع لازم يكون طويل حوالي متر طول في عرض
+
+438
+00:42:00,960 --> 00:42:03,060
+الأوتار
+
+439
+00:42:04,810 --> 00:42:08,510
+الذراعين طول في عرض متر في متر أو متر في 40
+
+440
+00:42:08,510 --> 00:42:13,230
+سنتي في حاجة اسمها رباط البطن أو حزام البطن أو
+
+441
+00:42:13,230 --> 00:42:17,070
+الصدر اللي بنسميه الرباط المستطيل الرباط المستطيل
+
+442
+00:42:17,070 --> 00:42:22,070
+هذا كيف بنسويه ولا ليش الرباط المستطيل هذا يا
+
+443
+00:42:22,070 --> 00:42:29,570
+أخوات المجدات لو جينا بدنا نربط حاجة كبيرة واحد
+
+444
+00:42:29,570 --> 00:42:37,480
+عنده كل بطنه وصدره محروق بدي ألفه يا جديش بدي
+
+445
+00:42:37,480 --> 00:42:43,320
+أربطة كتير جدا وجديش بدي أغلبه وأنا أجيبه و
+
+446
+00:42:43,320 --> 00:42:48,400
+أعدله يمين وأعدله شماله غلبي جدا فبنستخدمله حاجة
+
+447
+00:42:48,400 --> 00:42:53,180
+اسمها رباط المستطيل أو حزام البطن أو حزام الصدر
+
+448
+00:42:53,180 --> 00:42:58,420
+كيف هذا حزام البطن أو الصدر اللي بنعمله بجيب قطعة
+
+449
+00:42:58,420 --> 00:43:06,310
+قماش على عرض المسافة اللي أنا بدي إياها على عرض
+
+450
+00:43:06,310 --> 00:43:12,810
+المسافة اللي بدي أربطها وتكون طولها ثلث أضعاف
+
+451
+00:43:12,810 --> 00:43:18,050
+المنطقة اللي بدي ألفها يعني بدي ألف البطن أو الصدر
+
+452
+00:43:18,050 --> 00:43:24,170
+بدي أكون طولها ثلث أضعاف البطن أو الصدر مع العرض
+
+453
+00:43:24,170 --> 00:43:31,990
+المناسب عشان يكون ثلث أضعاف باجي بعمل كالقاطع
+
+454
+00:43:31,990 --> 00:43:33,250
+نعتبر أنه
+
+455
+00:43:38,070 --> 00:43:48,690
+هذا الرباط المستطيل فنقسمه ثلاثة هيك هيك ثلاثة
+
+456
+00:43:48,690 --> 00:43:55,250
+هيك تقريبا الثلاثة وبأجي بمزة عمزة بالمقص
+
+457
+00:44:25,150 --> 00:44:44,550
+ماشي الشجة الثانية نفس الحاجة الحين
+
+458
+00:44:44,550 --> 00:44:50,210
+مزقت هنا صارت زي شرشيب ماشي الحال الآن بدي أربط
+
+459
+00:44:50,210 --> 00:45:12,860
+مثلا صدر بطن فخد بحط المنطقة
+
+460
+00:45:12,860 --> 00:45:13,800
+على صدره
+
+461
+00:45:36,700 --> 00:45:45,120
+واضحة هيك؟ ولا مش واضحة؟ فيش بسوي الحين بجيب
+
+462
+00:45:45,120 --> 00:45:51,880
+القطعة هذه وبتنيها فبجيبها
+
+463
+00:45:51,880 --> 00:45:56,960
+هيك بالظبط اجتهان
+
+464
+00:45:58,540 --> 00:46:03,460
+الآن بجيب القطعة هذه و
+
+465
+00:46:03,460 --> 00:46:14,240
+بتنيها بتصير زي هيك الآن بجيب هذه و
+
+466
+00:46:14,240 --> 00:46:20,400
+بتنيها بتصير زي هيك ملاحظين؟ وبظل ماشي فيها
+
+467
+00:46:20,400 --> 00:46:24,340
+بالظبط زي هيك واحدة فوق أختها واحدة فوق أختها في
+
+468
+00:46:24,340 --> 00:46:31,640
+الآخر بحتاج فقط إلى قطعة plaster أو دبوس وبشبكها
+
+469
+00:46:31,640 --> 00:46:36,600
+مجرد بتشبكيها بقدر أروح وين ما كان بقدر أروح وين
+
+470
+00:46:36,600 --> 00:46:40,120
+ما كان إيش فائدته هذا الرباط المستطيل قلناه؟
+
+471
+00:46:40,120 --> 00:46:44,860
+الرباط المستطيل هذا فائدته أنه بيوفر جهد على اللي
+
+472
+00:46:44,860 --> 00:46:52,740
+بيغير وبيوفر جهد على المصاب أقل تكلفة ما بيكلفش شيء
+
+473
+00:46:52,740 --> 00:46:57,970
+قداش بيكلف قطعة قماش أنت .. وجه ملحفة وجه مخدة
+
+474
+00:46:57,970 --> 00:47:04,350
+خربانة وجه كذا قطعة مثلا جلباب بتقطيها وبتسويه
+
+475
+00:47:04,350 --> 00:47:12,290
+زي هي وبيدي دعم لمنطقة بطريقة مرتبة هذا اسمه رباط
+
+476
+00:47:12,290 --> 00:47:18,690
+المستطيل إذا left and keep بالعمل واضح في عندنا
+
+477
+00:47:18,690 --> 00:47:23,970
+رباط الحوادث أو الطوارئ رباط الحوادث أو الطوارئ كل
+
+478
+00:47:23,970 --> 00:47:28,800
+ما هو حواليك يعني بكل بساطة أنا ممكن زي ما أقول لكم في
+
+479
+00:47:28,800 --> 00:47:37,500
+الشارع أجيب سلك وأربط فيه ممكن أجيب يعني أحيانا
+
+480
+00:47:37,500 --> 00:47:40,660
+بيبقى ماشي في الشارع وبلاقي واحد بده أعلق له إيده
+
+481
+00:47:40,660 --> 00:47:47,460
+بحط إيده بين الظاهر إيش صرف الإيد تبدأ ولا لأ
+
+482
+00:47:47,460 --> 00:47:54,410
+أحيانا مثلا كشاط ممكن أستخدم منديل أي حاجة حواليك
+
+483
+00:47:54,410 --> 00:47:58,570
+أنتوا ملاحظين إيش بنستخدم إيش بنستخدم نشتغل
+
+484
+00:47:58,570 --> 00:48:03,090
+بالصحافة أولى راقع جدا ومحترم جدا وكويس جدا وبس
+
+485
+00:48:03,090 --> 00:48:07,450
+الشيء المتوفر أيوة تقولي ما عنديش أدوات ولو
+
+486
+00:48:07,450 --> 00:48:11,830
+عندي أدوات كانت عاملة بتكون ما عندكيش معرفة لازم
+
+487
+00:48:11,830 --> 00:48:21,050
+تشتغلي بالموجود نعم فرباط الحوادث والطوارئ كل شيء
+
+488
+00:48:21,050 --> 00:48:28,790
+حولك ممكن تستخدميه حتى فروق الصخر كيف بنستخدم
+
+489
+00:48:28,790 --> 00:48:32,210
+الرباط؟
+
+490
+00:48:32,210 --> 00:48:40,850
+الرباط لازم يكون محكم شو يعني محكم؟ لا هو حزج ولا
+
+491
+00:48:40,850 --> 00:48:46,850
+هو رخو ليه؟ إذا كان حزج بسكر على الدورة الدموية
+
+492
+00:48:46,850 --> 00:48:53,590
+وإذا كان رخو bigger bigger حطينا هذا الرباط ومشينا
+
+493
+00:48:53,590 --> 00:49:02,010
+فيه ما وصلش الباب اللي هو إيش واجع وعملنا مشكلة إذا
+
+494
+00:49:02,010 --> 00:49:07,110
+اضطرينا أن نعمل على الأطراف بدنا نخلي الأصابع من
+
+495
+00:49:07,110 --> 00:49:12,750
+بعيدنا أحيانا تضطري أنك تغطي الإصبع بس يكون إصبع
+
+496
+00:49:12,750 --> 00:49:18,090
+واحد يعني انضرب الإظفر وانضرب مقدمة الإصبع مضغطه
+
+497
+00:49:18,090 --> 00:49:25,410
+فبغطي إصبع واحد بعد
+
+498
+00:49:25,410 --> 00:49:31,030
+ما أحط رباط لازم أراعي أنه ما يصيرش تغيير اللون
+
+499
+00:49:31,030 --> 00:49:41,580
+ما يصيرش ورم ما يصيرش خذلان أو شعور كأنه فيه نملا أو
+
+500
+00:49:41,580 --> 00:49:45,040
+أي حاجة تدل أن الأوعية الدموية اتأثرت أو الجهاز 
+
+501
+00:49:45,040 --> 00:49:49,480
+العصبي اتأثر وإذا صار واحد من هدول أجرّيه بدون 
+
+502
+00:49:49,480 --> 00:49:55,700
+مفكر إيش بساوي؟ مفكر رباط لأنه أكيد بيكون فيه مشكلة
+
+503
+00:49:55,700 --> 00:49:59,820
+كمان من الحاجات المهمة أنه قبل ما أحط رباط على
+
+504
+00:49:59,820 --> 00:50:04,940
+الأطراف بدي أجيس النبض وبعد ما أحط رباط على الطرف
+
+505
+00:50:04,940 --> 00:50:09,240
+برضه بدي أجيس النبض لأنه أنا ممكن أجيس النبض الحين
+
+506
+00:50:09,720 --> 00:50:14,520
+وبعد ما أحطها تغير مجلد أو مش مبين بكون أنا حاطط
+
+507
+00:50:14,520 --> 00:50:20,160
+رباط حزج مسكر على القوية الدموية وزي ما قلنا
+
+508
+00:50:20,160 --> 00:50:25,440
+مانحطش على الرقبة رباط لأن الرقبة بتنخنق إذا
+
+509
+00:50:25,440 --> 00:50:33,800
+حاطينا البني آدم رباط على اللي هو رقبته كيف
+
+510
+00:50:33,800 --> 00:50:41,110
+بنستخدم الرباط المثلث الآن هاي هذا تقريباً مما حولنا
+
+511
+00:50:41,110 --> 00:50:46,430
+استخدمنا كرباط مثلث كيف بدنا نعلق فيه إيد أو رجل
+
+512
+00:50:46,430 --> 00:50:50,190
+الزاوية
+
+513
+00:50:50,190 --> 00:50:55,150
+القايمة بدي أحطها وين؟ عكس الإيد المخبوطة هيبقى بدي
+
+514
+00:50:55,150 --> 00:51:00,170
+أعلق هات بحط الزاوية هين الوتر بيجي على الجبهة
+
+515
+00:51:00,170 --> 00:51:04,350
+ملاحظين هذه الزاوية وهذه الوتر وهذه الإيد اللي
+
+516
+00:51:04,350 --> 00:51:10,740
+بدي أعلقها كل بساطة هذه الجرنة بحطها وين؟ عشان كده
+
+517
+00:51:10,740 --> 00:51:20,660
+كيف صار كده؟ هاي رباط مسلسل فعلّبنا ديّ هي صار إيه؟
+
+518
+00:51:20,660 --> 00:51:25,420
+رباط مسلسل فعلّبنا ديّ من وين جبناه؟ من ما حول...
+
+519
+00:51:25,420 --> 00:51:32,180
+من ما حول هذا بالنسبة لرباط المسلسل رباط المسلسل
+
+520
+00:51:32,180 --> 00:51:39,280
+عبارة عن قطعة من قماش منديل بتجسميه نصين أو بتتنيه
+
+521
+00:51:39,280 --> 00:51:43,780
+على ثانيتين وبيصير أحسن رباط مثلث في البلد
+
+522
+00:51:43,780 --> 00:51:52,820
+بتستخدميه بالطريقة اللي بدكِ إيها نعم
+
+523
+00:51:52,820 --> 00:51:56,840
+يعملوا
+
+524
+00:51:56,840 --> 00:52:04,040
+كتفه هذا بيسموه رباط شكل ثمانية مش اللي بيشد
+
+525
+00:52:04,040 --> 00:52:07,140
+كتفه زي هيه اه هذا اسمه رباط شكل ثمانية
+
+526
+00:52:13,200 --> 00:52:20,400
+مش الحال إذا هذا الرباط المثلث بنستخدمه لتعليق الإيد
+
+527
+00:52:20,400 --> 00:52:25,320
+زي ما أنت شايفينه يحضر
+
+528
+00:52:25,320 --> 00:52:28,900
+بوضع أحد الأطراف زي ما شوفته على الكتف السليم
+
+529
+00:52:28,900 --> 00:52:33,260
+ويطرق الطرف الثاني مقابل لتدلق الأمام زي ما شوفته
+
+530
+00:52:33,260 --> 00:52:38,850
+يظل على الزاوية وبعدين الوتر بالثالث يشد الطرف و
+
+531
+00:52:38,850 --> 00:52:44,050
+يثبت الطرف الثالث وبتخلي الأصابع تظل هائش مكشوف
+
+532
+00:52:44,050 --> 00:52:48,170
+أربطة
+
+533
+00:52:48,170 --> 00:52:54,810
+الرأس ممكن استخدام أربطة مسلس أربطة رأس زي كده
+
+534
+00:52:54,810 --> 00:53:05,590
+يعني هذا الرباط مسلس مش هي مسلس لو جيت أنا أطبّق أنت 
+
+535
+00:53:05,590 --> 00:53:10,370
+تتصور إن هذا منديل يعني ماشي لو جيت إذا لفيته
+
+536
+00:53:10,370 --> 00:53:18,370
+بالشكل هذا و
+
+537
+00:53:18,370 --> 00:53:29,730
+حطيته على رأسي مش ممكن يلف الراس ممكن يلف الرأس و
+
+538
+00:53:29,730 --> 00:53:35,890
+بنربطه وبيصير إيه؟ ماسك الرأس هذا بالنسبة لطريقة
+
+539
+00:53:35,890 --> 00:53:40,630
+إذا كان حاطينا رباط على وين؟ على الرأس لو كان بدي
+
+540
+00:53:40,630 --> 00:53:47,030
+أشد شجة الوجه مثلاً ده حاطينا غيار وبدنا نحطه برضه
+
+541
+00:53:47,030 --> 00:53:53,990
+نفس رباط المثلث هذا نفسه ممكن أستخدمه للوجه بالشكل
+
+542
+00:53:53,990 --> 00:53:57,810
+هذا هو
+
+543
+00:53:57,810 --> 00:53:58,130
+بعيد
+
+544
+00:54:00,940 --> 00:54:06,680
+لحظين يعني كنت... كنت أتصرفوا يعني بعجبني أنا إن
+
+545
+00:54:06,680 --> 00:54:10,760
+الواحد دي يتصرف ومات بالبشر وده إيش رأيكوا؟
+
+546
+00:54:10,760 --> 00:54:17,920
+يتصرفوا بإيش؟ بما يتواجد حولكم فالبعض المثلثات اللي
+
+547
+00:54:17,920 --> 00:54:24,320
+قدت استخدامات للحالات
+
+548
+00:54:24,320 --> 00:54:25,000
+اللي صارت
+
+549
+00:54:33,750 --> 00:54:43,570
+اربط الوجه ده جانب ده عملنا الذكرات أربطة
+
+550
+00:54:43,570 --> 00:54:48,070
+الكوح أو الركبة كمان الرباط المثلث ممكن استخدم من
+
+551
+00:54:48,070 --> 00:54:53,470
+الكوح والركبة كيف ممكن استخدم من الكوح والركبة
+
+552
+00:54:53,470 --> 00:55:03,070
+نفس الرباط بلفّه الآن يعني اعتبره إن هذا الرباط المثلث
+
+553
+00:55:03,070 --> 00:55:11,810
+جبته بالشكل هذا ماشي فبعدي بلفّه كالعادي الأخت
+
+554
+00:55:11,810 --> 00:55:16,190
+اللي سألت عن شكل ثمانية هاي بدي أعمل لكم رباط شكل
+
+555
+00:55:16,190 --> 00:55:27,630
+ثمانية ماشي الحال هاي هيك وبعدي
+
+556
+00:55:27,630 --> 00:55:34,650
+بجيبه هان إيش صار؟ بالإنكليزي إيه الشكل ده؟ ثمانية
+
+557
+00:55:34,650 --> 00:55:39,350
+بالإنكليزي مظبوط؟ هي شكل ثمانية بالإنكليزي إيش
+
+558
+00:55:39,350 --> 00:55:44,090
+ميزة هذا الشغل؟ إنه لو حركت قوي اللي ربنا خلّقه
+
+559
+00:55:44,090 --> 00:55:48,690
+للحركة بفكر لكن لو أني مديته وعملت زي هيك و
+
+560
+00:55:48,690 --> 00:55:53,230
+حركت قوي بتطلع بقول لأ أنا زلزل مهال عملناش كده
+
+561
+00:55:53,230 --> 00:55:58,290
+نفس الحركة ممكن أعملها وين؟ في الرقبة تن الرقبة و
+
+562
+00:55:58,290 --> 00:56:03,230
+بعملها نفس الحركة بعملها في وين؟ في الكاحل وغلاق
+
+563
+00:56:03,230 --> 00:56:06,810
+تبارك الله وكل هذه الشغلات بنستخدمها للكور و
+
+564
+00:56:06,810 --> 00:56:13,290
+الرقبة والكاحل لأنه يعني هذه مناطق اسمها مناطق مش
+
+565
+00:56:13,290 --> 00:56:18,850
+ثابتة مناطق متحركة عشان هيك بقى استخدمها في السوق
+
+566
+00:56:29,560 --> 00:56:37,960
+هذه الطرق اللي كيف ممكن نستخدم اللي هو
+
+567
+00:56:37,960 --> 00:56:46,280
+أربطة
+
+568
+00:56:46,280 --> 00:56:52,260
+تثبيت الغيارات أربطة تثبيت الغير أربطة تثبيت
+
+569
+00:56:52,260 --> 00:56:57,480
+الغير اللي زي هذاهذا ممكن نستخدمه لتثبيت الغيار
+
+570
+00:56:57,480 --> 00:57:02,660
+كمان ممكن هذا نستخدمه لإيش؟ لتثبيت الغيار طب
+
+571
+00:57:02,660 --> 00:57:08,520
+ماعنديش لا هذا ولا هذا إيش أساوي؟ قطعة قماش من شرشة
+
+572
+00:57:08,520 --> 00:57:17,420
+أو من أي جلباب قديم مش لازم أو من منديل بقى قص
+
+573
+00:57:17,420 --> 00:57:22,280
+العرض المطلوب خمسة سنتي سبعة سنتي ثلاثة سنتي
+
+574
+00:57:22,280 --> 00:57:27,380
+عشرة سنتي بالطول المناسب وبستخدمها رباط ولا مش
+
+575
+00:57:27,380 --> 00:57:35,100
+هيك فأربطة الغيارات بنستخدم أربطة اللي هو يوضع طرف
+
+576
+00:57:35,100 --> 00:57:40,740
+الرباط فوق أسفل الغير ويثبت مؤقتاً بأصابع المسل
+
+577
+00:57:40,740 --> 00:57:46,140
+بتحط للرباط وبتلفي بإصبعك وبعدين بتلفي لما تيجي
+
+578
+00:57:46,140 --> 00:57:48,840
+الطرف فوق إخوتي ولاحظته هنا لما جيت ألف على إيدي
+
+579
+00:57:50,060 --> 00:57:55,320
+غلبني لاحظتُن لاحظتُن كل ما... لكن لو أنا بشتغل لو
+
+580
+00:57:55,320 --> 00:58:02,620
+أحد بحط إصبعي وبلف واضحة؟ هذه معناها اللي بتحطي
+
+581
+00:58:02,620 --> 00:58:08,140
+أطراف أصابع يلف
+
+582
+00:58:08,140 --> 00:58:11,660
+الرباط فوق أسفل جزء من الغيار حطينا الغيار بجي من
+
+583
+00:58:11,660 --> 00:58:18,580
+الغيار وتحت بحط رباط عشان إيش؟ ييجي باقي الرباط
+
+584
+00:58:18,580 --> 00:58:23,590
+فوق الغيار ويمسكوه يلف رباط حلزونياً من أسفل إلى
+
+585
+00:58:23,590 --> 00:58:30,310
+أعلى على أن لا يغطي كل لفة غط الأخرى ماشي؟ وهذا
+
+586
+00:58:30,310 --> 00:58:35,850
+بيسموه رباط حلزوني مغلق رباط حلزوني إيش؟ مغلق زي
+
+587
+00:58:35,850 --> 00:58:41,650
+كيف رباط حلزوني مغلق؟ بصوا جاي هاي لفة اللفة
+
+588
+00:58:41,650 --> 00:58:46,850
+الثانية بتيجي بتغطيها زي شكل الحلزون واللي بعدها
+
+589
+00:58:46,850 --> 00:58:52,740
+بتغطيها والبعدها إيش بتساوي؟ بتغطيها إيش صار؟ زي شكل
+
+590
+00:58:52,740 --> 00:59:14,780
+الحلزون مغلق هذا اسمه رباط حلزوني مغلق فأنا
+
+591
+00:59:14,780 --> 00:59:15,480
+ما خلصناه
+
+592
+00:59:18,600 --> 00:59:25,260
+اربط الأصابع ربط الأصابع لو أنا بدأت أربط الأصابع
+
+593
+00:59:25,260 --> 00:59:35,160
+ممكن جداً أجيب قطعة قماش اليوم في أربطة إذا شفتوا
+
+594
+00:59:35,160 --> 00:59:39,960
+هدول اللي بيستخدموها زي الأسطوانات اللي بيلفوا
+
+595
+00:59:39,960 --> 00:59:47,660
+فيها الشعر زي الأسطوانة تيجي قد الإصبع ماشي في
+
+596
+00:59:47,660 --> 00:59:51,980
+اليوم أربطة زي اللي بيحطوه زي جرابين اللي بيحطوه
+
+597
+00:59:51,980 --> 00:59:55,620
+تحت الجبس تبعين الجبس مش عندهم حاجات زي الجرابين
+
+598
+00:59:55,620 --> 01:00:02,560
+في منهم رفيع فلو حطيت الحديد هذه وتحطيها فوق
+
+599
+01:00:02,560 --> 01:00:09,780
+الإصبع وتحطي الرفيع اللي بيسمّوه فوقها واطلعي
+
+600
+01:00:09,780 --> 01:00:13,940
+الحديد فوق بيظل ماسك على الإصبع وبتلفيها لفة و
+
+601
+01:00:13,940 --> 01:00:18,640
+بتدخليها ثاني وبتلفيها وبتطلعيها فتطلع لك رباط
+
+602
+01:00:18,640 --> 01:00:23,040
+مرتب جداً هذا إذا بدك تربطي أصابعك بدك تستخدم هذا
+
+603
+01:00:23,040 --> 01:00:28,200
+رباط الشاشة في أكثر من طريقة استخدام رباط الشاشة
+
+604
+01:00:28,200 --> 01:00:34,020
+طريقة الأولى أنه أمشي فوق تحت فوق تحت مرتين ثلاثة
+
+605
+01:00:34,020 --> 01:00:39,780
+وبعدين ألف الرباط لو لفيت رباط ومشيت حيجع فبروح
+
+606
+01:00:39,780 --> 01:00:44,860
+وأنا بنزل على كوره على ال... آخر الإيد من تحت
+
+607
+01:00:44,860 --> 01:00:50,860
+عشان لا يسحبش هذه حاجة واحد ثاني إن ألفه عادي بدون
+
+608
+01:00:50,860 --> 01:00:57,700
+ما ألف رايح يعني وبرضه بلفّه من تحت عشان ما ينزل
+
+609
+01:01:05,550 --> 01:01:11,010
+رباط الكاحل اللي قلناه إيش؟ على شكل الخرق تمام
+
+610
+01:01:11,010 --> 01:01:14,650
+واضح؟ في أي سؤال في اللي قلناه؟ 
diff --git a/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/o7VV4VyEA2k_raw.json b/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/o7VV4VyEA2k_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..bd97a507d0be68e9fd97cb02e4a30ce48bc44759
--- /dev/null
+++ b/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/o7VV4VyEA2k_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 2909, "start": 18.85, "end": 29.09, "text": "الله الرحمن الرحيم ان شاء الله اليوم هنبدأ في تكميل اللى أخدناه الدور الفاتح هنبدأ في موضوع التشنجات", "tokens": [6027, 43761, 34892, 5016, 27842, 34892, 5016, 32640, 16472, 13412, 16606, 21984, 45595, 20498, 8032, 1863, 44510, 10721, 8978, 6055, 24793, 26895, 13672, 7578, 5551, 9778, 3215, 8315, 3224, 32748, 13063, 27188, 9307, 5016, 8032, 1863, 44510, 10721, 8978, 3714, 2407, 11242, 45367, 16712, 8592, 1863, 7435, 9307], "avg_logprob": -0.12786990282486896, "compression_ratio": 1.605263157894737, "no_speech_prob": 2.7418136596679688e-06, "words": [{"start": 18.85, "end": 19.21, "word": "الله", "probability": 0.5626220703125}, {"start": 19.21, "end": 19.59, "word": " الرحمن", "probability": 0.83056640625}, {"start": 19.59, "end": 20.03, "word": " الرحيم", "probability": 0.99072265625}, {"start": 20.03, "end": 21.17, "word": " ان", "probability": 0.62548828125}, {"start": 21.17, "end": 21.39, "word": " شاء", "probability": 0.984375}, {"start": 21.39, "end": 21.49, "word": " الله", "probability": 0.96240234375}, {"start": 21.49, "end": 21.75, "word": " اليوم", "probability": 0.957763671875}, {"start": 21.75, "end": 22.37, "word": " هنبدأ", "probability": 0.93896484375}, {"start": 22.37, "end": 22.81, "word": " في", "probability": 0.8115234375}, {"start": 22.81, "end": 23.49, "word": " تكميل", "probability": 0.9158528645833334}, {"start": 23.49, "end": 24.97, "word": " اللى", "probability": 0.8173828125}, {"start": 24.97, "end": 25.39, "word": " أخدناه", "probability": 0.91611328125}, {"start": 25.39, "end": 25.57, "word": " الدور", "probability": 0.909912109375}, {"start": 25.57, "end": 26.11, "word": " الفاتح", "probability": 0.6941731770833334}, {"start": 26.11, "end": 26.53, "word": " هنبدأ", "probability": 0.9456787109375}, {"start": 26.53, "end": 26.65, "word": " في", "probability": 0.7626953125}, {"start": 26.65, "end": 27.17, "word": " موضوع", "probability": 0.9888916015625}, {"start": 27.17, "end": 29.09, "word": " التشنجات", "probability": 0.97275390625}], "temperature": 1.0}, {"id": 2, "seek": 9424, "start": 64.46, "end": 94.24, "text": "ماشي الحال؟ لو سألنا سؤال إيش يعني إيه التشنجات؟ والله إيش مال فلان؟ والله بتشنج إيش يعني بتشنج؟ مش عارف منكوا شوفته؟ شد عضلي جوي تقلصات عضلية إيش كمان؟ بتشنج واحد بفقد وعيه وكل عضلاته مشدودة", "tokens": [2304, 33599, 1829, 21542, 6027, 22807, 45164, 8608, 10721, 1211, 8315, 8608, 33604, 6027, 11933, 1829, 8592, 37495, 22653, 11933, 1829, 3224, 16712, 8592, 1863, 7435, 9307, 22807, 16070, 43761, 11933, 1829, 8592, 3714, 6027, 6156, 1211, 7649, 22807, 16070, 43761, 39894, 8592, 1863, 7435, 11933, 1829, 8592, 37495, 22653, 39894, 8592, 1863, 7435, 22807, 37893, 6225, 9640, 5172, 9154, 4117, 14407, 13412, 38688, 47395, 22807, 13412, 3215, 6225, 11242, 20292, 10874, 45865, 6055, 4587, 1211, 9381, 9307, 6225, 11242, 1211, 10632, 11933, 1829, 8592, 9122, 2304, 7649, 22807, 39894, 8592, 1863, 7435, 36764, 24401, 4724, 5172, 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على القوية الدموية و زي ما قلنا مانحطش على الرجبة رباط لان الرجبة بتنخنق اذا حاطينا البني ادم رباط على اللي هو رجبته كيف بنستخدم الرباط المثلث الان هاي هذا تقريبا", "tokens": [2407, 39182, 19446, 1975, 5016, 9566, 11296, 6055, 17082, 13546, 3714, 7435, 1211, 3215, 1975, 2407, 37893, 3714, 3555, 9957, 4724, 30544, 1975, 8315, 11331, 41193, 9566, 12602, 3555, 41193, 11331, 11622, 7435, 47524, 37983, 15844, 25062, 2407, 10632, 32748, 2304, 2407, 10632, 4032, 30767, 1829, 19446, 12174, 1211, 8315, 19446, 1863, 5016, 9566, 8592, 15844, 34892, 7435, 49401, 12602, 3555, 41193, 5296, 7649, 34892, 7435, 49401, 39894, 1863, 9778, 1863, 4587, 1975, 15730, 11331, 41193, 1829, 8315, 29739, 22653, 1975, 40448, 12602, 3555, 41193, 15844, 13672, 1829, 31439, 12602, 7435, 3555, 47395, 9122, 33911, 44945, 14851, 9778, 40448, 34892, 3555, 41193, 9673, 12984, 1211, 12984, 2423, 7649, 8032, 47302, 23758, 6055, 4587, 16572, 3555, 995], "avg_logprob": -0.26883013737507355, "compression_ratio": 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0.94189453125}], "temperature": 1.0}, {"id": 127, "seek": 367465, "start": 3665.55, "end": 3674.65, "text": "ارباط الكاحل اللي قلناه إيش على شكل الخرق تمام واضح؟ في أي سؤال في اللي قلناه؟", "tokens": [9640, 3555, 41193, 33251, 39319, 1211, 13672, 1829, 12174, 1211, 8315, 3224, 11933, 1829, 8592, 15844, 13412, 28820, 33962, 2288, 4587, 46811, 10943, 4032, 46958, 5016, 22807, 8978, 36632, 8608, 33604, 6027, 8978, 13672, 1829, 12174, 1211, 8315, 3224, 22807], "avg_logprob": -0.21303354240045314, "compression_ratio": 1.4536082474226804, "no_speech_prob": 1.7881393432617188e-07, "words": [{"start": 3665.55, "end": 3666.07, "word": "ارباط", "probability": 0.7100423177083334}, {"start": 3666.07, "end": 3666.69, "word": " الكاحل", "probability": 0.7937825520833334}, {"start": 3666.69, "end": 3667.11, "word": " اللي", "probability": 0.754150390625}, {"start": 3667.11, "end": 3667.55, "word": " قلناه", "probability": 0.9141845703125}, {"start": 3667.55, "end": 3667.91, "word": " إيش", "probability": 0.7603352864583334}, {"start": 3667.91, "end": 3669.81, "word": " على", "probability": 0.66259765625}, {"start": 3669.81, "end": 3670.15, "word": " شكل", "probability": 0.984130859375}, {"start": 3670.15, "end": 3670.59, "word": " الخرق", "probability": 0.5924479166666666}, {"start": 3670.59, "end": 3671.01, "word": " تمام", "probability": 0.890380859375}, {"start": 3671.01, "end": 3672.65, "word": " واضح؟", "probability": 0.8271484375}, {"start": 3672.65, "end": 3673.15, "word": " في", "probability": 0.822265625}, {"start": 3673.15, "end": 3673.33, "word": " أي", "probability": 0.75439453125}, {"start": 3673.33, "end": 3673.67, "word": " سؤال", "probability": 0.98779296875}, {"start": 3673.67, "end": 3673.79, "word": " في", "probability": 0.85400390625}, {"start": 3673.79, "end": 3673.89, "word": " اللي", "probability": 0.97412109375}, {"start": 3673.89, "end": 3674.65, "word": " قلناه؟", "probability": 0.9283203125}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 3675.208, "duration_after_vad": 3450.2199999999866}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/xHyOqBpHDRs.srt b/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/xHyOqBpHDRs.srt
new file mode 100644
index 0000000000000000000000000000000000000000..b15ebf51ae0e5b504af0f8b93db4504e304ca2a6
--- /dev/null
+++ b/PL9fwy3NUQKwa9WiYyfamUPAJphmPPRiCZ/xHyOqBpHDRs.srt
@@ -0,0 +1,3732 @@
+1
+00:00:19,560 --> 00:00:25,340
+اليوم إن شاء الله هنتكلم عن الولادة الطارئة، إيش 
+
+2
+00:00:25,340 --> 00:00:29,060
+عن الولادة الطارئة؟
+
+3
+00:00:29,060 --> 00:00:32,660
+ممكن 
+
+4
+00:00:32,660 --> 00:00:36,360
+في غير مواعدها،
+
+5
+00:00:36,360 --> 00:00:41,700
+ممكن ست، تلت، سابع أو ثامن، ولادة بتقدرش تصل
+
+6
+00:00:41,700 --> 00:00:47,660
+للمستشفى أو بنقدرش نجيب لها أخصائي ولادة أو
+
+7
+00:00:47,660 --> 00:00:55,500
+أخصائية ولادة أو داية بالظبط ست بتولد في مكان معين
+
+8
+00:00:55,500 --> 00:01:02,120
+فبنسمي هذا إيش الولادة الطارئة يعني أنا بدي أودي
+
+9
+00:01:02,120 --> 00:01:07,720
+حالة تلت الدباب واقف على رأس الشارع والطخ شغال
+
+10
+00:01:07,720 --> 00:01:11,380
+والست قاعدة بتولد بنتصل على الإسعاف ممنوع يصل
+
+11
+00:01:11,380 --> 00:01:16,580
+فبسير حد عبارة عن ولادة إيش طالع مين بده يشتغلها؟
+
+12
+00:01:16,580 --> 00:01:20,540
+بده يشتغلها أنا الذاكرين يعني أظن فاطمة رضي الله 
+
+13
+00:01:20,540 --> 00:01:26,080
+عنها لما جالها عليها لكي في أجر أنسقه الله لكي؟
+
+14
+00:01:26,080 --> 00:01:31,780
+مين أحد الصحابيات أو أمهات المؤمنين اللي خشت
+
+15
+00:01:31,780 --> 00:01:41,220
+ولدت ست؟ مارت عنكوا قصة وزي هي؟ أحد الصحابيات قال
+
+16
+00:01:41,220 --> 00:01:44,920
+لزوجها هلك في أجر أنسقه الله لك؟ قالت له إيش؟ قال
+
+17
+00:01:44,920 --> 00:01:48,860
+له في امرأة قاعدة بتولد فراحت ولدتها يعني لا هي
+
+18
+00:01:48,860 --> 00:01:53,200
+دكتورة ولا عمرها يمكن ولدت إنما الولادة الطبيعية
+
+19
+00:01:53,200 --> 00:01:59,220
+يا أخوات المجدات ولادة بتبدأ وتتقدم وتنتهي بتسهيل
+
+20
+00:01:59,220 --> 00:02:02,380
+من الله سبحانه وتعالى الله سبحانه وتعالى إيش
+
+21
+00:02:02,380 --> 00:02:11,490
+بيقول بيقول ثم يسّر السبيل، يسّر صورة عبس وتولى ثم 
+
+22
+00:02:11,490 --> 00:02:15,370
+السبيل يسّره يعني الله سبحانه وتعالى يسر أما البني
+
+23
+00:02:15,370 --> 00:02:17,990
+آدم ينزل من بطن أمه، طب بعد ما ينزل البني آدم من
+
+24
+00:02:17,990 --> 00:02:23,630
+بطن أمه وكل العالم تعرف ترجع في إمكانية؟ مش
+
+25
+00:02:23,630 --> 00:02:28,320
+إمكانية، ولو ما فيش تسهيل ممكن ينزل؟ ما فيش إمكانية
+
+26
+00:02:28,320 --> 00:02:33,220
+أنه ينزل فالولادة الطبيعية الولادة اللي بتمشي تبدأ
+
+27
+00:02:33,220 --> 00:02:38,460
+وبتتقدم وبتنتهي بسهولة يعني تعال لف العالم في
+
+28
+00:02:38,460 --> 00:02:43,580
+في كل العالم مستشفى
+
+29
+00:02:43,580 --> 00:02:50,100
+لولادة مثلا قرانب فيه؟
+
+30
+00:02:50,100 --> 00:02:55,480
+عارفين أن القرانب بتولد كل أربعين يوم من ثمانية ال
+
+31
+00:02:55,480 --> 00:03:03,960
+11 عرفتها كل 40 يوم ومع ذلك ما فيش مستشفيات ليش الله
+
+32
+00:03:03,960 --> 00:03:07,880
+سبحانه وتعالى ميسّرها فالولادة هذه تسهيل من الله
+
+33
+00:03:07,880 --> 00:03:11,980
+سبحانه وتعالى إلا أنه يعني هذا بقى بمنع أنه لازم
+
+34
+00:03:11,980 --> 00:03:19,060
+السيدة تعمل متابعة للحمل اليوم في كل البلد ملانة
+
+35
+00:03:19,060 --> 00:03:23,120
+مراكز متابعة الحمل وفي حاجة اسمها الحمل الخطير وفي
+
+36
+00:03:23,120 --> 00:03:26,300
+حاجة اسمها الحمل الطبيعي ولا مش هيك الستات اللي
+
+37
+00:03:26,300 --> 00:03:31,500
+بيروحوا بتابعوها بيسألوها ضغط بيسألوها قوة الدم
+
+38
+00:03:31,500 --> 00:03:38,120
+بيسألوها، إيش اسمه؟ كل السكر، فحوصات دموية،
+
+39
+00:03:38,120 --> 00:03:40,720
+بيعملوله متابعة بعدين شوية بيصيروا يتابعوا
+
+40
+00:03:40,720 --> 00:03:46,300
+للجنين كمان، قداش صار حجمه، قداش زرمزادها بيزيد،
+
+41
+00:03:46,300 --> 00:03:51,500
+بيزيدش، ضغطها، سكريها، الزلال، كل هذه الشغلات
+
+42
+00:03:52,010 --> 00:03:56,770
+بيتابعوها على أساس أنه إيش يصير الحمل إن شاء الله
+
+43
+00:03:56,770 --> 00:04:03,550
+حمل مرتب فالولادة الطارئة يعني بتصير تتجاوز كل
+
+44
+00:04:03,550 --> 00:04:07,010
+الخطوط اللي احنا ممكن نعمل يعني أنا واحد من الناس
+
+45
+00:04:07,010 --> 00:04:14,450
+أجريت عملية ولادة تلت مرات مش بخاطر غصب عنه ولادة
+
+46
+00:04:14,450 --> 00:04:19,910
+طارئة كنت في مستشفى من المستشفيات في جنب قسم
+
+47
+00:04:19,910 --> 00:04:25,250
+الولادة طبعا ولادة بسيطة كانت ما بيولدوش كتير ففي
+
+48
+00:04:25,250 --> 00:04:27,950
+أثناء وجودي هناك كنت في جنب في قسم الحضانة
+
+49
+00:04:27,950 --> 00:04:34,450
+بشتغل الست جنبنا صرخت صرخت فالدكتور مش موجود
+
+50
+00:04:34,450 --> 00:04:41,840
+فروحت جراي إذا الجنين بيطلع استقبلت الجنين وكانت
+
+51
+00:04:41,840 --> 00:04:45,280
+حاملة بتوأم هذا أول ولادة لسه في حياتي بعملها يعني
+
+52
+00:04:45,280 --> 00:04:50,120
+وكانت حاملة بتوأم طلعت الجنين الأول واتصلت على 
+
+53
+00:04:50,120 --> 00:04:53,560
+الطبيب أجى اتكمل وطلعت جنين الثاني هذا الولادة
+
+54
+00:04:53,560 --> 00:04:58,220
+الأولى الولادة الثانية كنت في مستشفى ناصر اشتغلت
+
+55
+00:04:58,220 --> 00:05:01,820
+فترة طويلة في مستشفى ناصر ولبست البالطو وبلف على
+
+56
+00:05:01,820 --> 00:05:08,110
+الأجسام كون بجت يعني مسئول مستشفى فشافني سواج يا
+
+57
+00:05:08,110 --> 00:05:11,210
+أخي يا أخي يا أخي نعم قال لي الله يرضى عليك في
+
+58
+00:05:11,210 --> 00:05:16,370
+معي حالة في السيارة مستوية حالتها إيه؟ قال لي حالة
+
+59
+00:05:16,370 --> 00:05:20,990
+ولادة طلعت وصلت لامرأة على آخر استقبلت الجنين برضه
+
+60
+00:05:20,990 --> 00:05:24,490
+في وسط السيارة في وسط الاستقبال لفينا على حالنا
+
+61
+00:05:24,490 --> 00:05:29,830
+واستقبلنا الجنين وولدناها وقعدت هال بنتي بكرية أي
+
+62
+00:05:29,830 --> 00:05:31,310
+ابن هالسنة كان في توجيهي
+
+63
+00:05:35,020 --> 00:05:38,820
+راحت على عيادة الوكالة في الـ bridge فقالوا لها
+
+64
+00:05:38,820 --> 00:05:42,580
+معليش أنت بكرية لازم تروحي على الشفاء بدل ما تروحي
+
+65
+00:05:42,580 --> 00:05:45,740
+على الشفاء سحبت حالها وأجت عندنا في انصارات من
+
+66
+00:05:45,740 --> 00:05:49,680
+الـ bridge لانصارات ووصلت لنهيانة ما فيش نفس يعني زي
+
+67
+00:05:49,680 --> 00:05:53,860
+ما أبونا جاعتها وولدتها وابن أسنة كان في توجيه
+
+68
+00:05:53,860 --> 00:05:59,020
+هدول تلت حالات فُربت فإذا كان عندك واحد فكرة أن
+
+69
+00:05:59,020 --> 00:06:02,520
+إن شاء الله ربنا مع تسهيل المولد طبعا ربنا ياش بسهل
+
+70
+00:06:03,060 --> 00:06:06,340
+الولادة الطارئة هي الولادة اللي أنت بتضطري تعمليها
+
+71
+00:06:06,340 --> 00:06:12,020
+يعني عشان أنه ما فيش إمكانية إلا تدخليه في توليدها
+
+72
+00:06:12,020 --> 00:06:17,940
+كيف أنت بدك تعمليها تقدري أن هذه الست في حالة
+
+73
+00:06:17,940 --> 00:06:24,720
+ولادها؟ مين بتعرف؟ في كان حد متجوز وخلفات؟ أه؟
+
+74
+00:06:24,720 --> 00:06:33,500
+فيه؟ كام نفر؟ ربنا يبعت لهم بأخوه يجعله من الصالحين
+
+75
+00:06:33,500 --> 00:06:38,760
+كيف بتحس أن أنت حالة ولادة؟
+
+76
+00:06:38,760 --> 00:06:49,340
+طب
+
+77
+00:06:49,340 --> 00:06:54,240
+المغز هذا إيه مواصفاته؟
+
+78
+00:06:54,240 --> 00:07:00,650
+سمعتوا كلمة ورا بعض؟ كلمة ورا بعض مهمة جدا طبعا هي
+
+79
+00:07:00,650 --> 00:07:04,770
+كان بيجيها مغص من خمس أشهر يوم قبل الولادة بس مش
+
+80
+00:07:04,770 --> 00:07:11,490
+مش ورا بعض بالظبط علامات أنه الست في حالة ولادة
+
+81
+00:07:11,490 --> 00:07:21,070
+أول حاجة البكرية بيجيها طلق أو تقلصات رحم كل
+
+82
+00:07:21,070 --> 00:07:27,180
+دقيقتين طلقة وبتستمر لدقيقة إلا ربع مش هيك؟ دقيقة إلا
+
+83
+00:07:27,180 --> 00:07:32,880
+ربع إذا صارت الست بدأت من هذه اللحظة تلجأ أو تقل 
+
+84
+00:07:32,880 --> 00:07:37,520
+صاحب كل دقيقتين لمدة خمس وأربع ثانية تحتاج إلى
+
+85
+00:07:37,520 --> 00:07:41,960
+ساعتين حتى تلت في الوضع الطبيعي ساعتين طبعا
+
+86
+00:07:41,960 --> 00:07:48,680
+الساعتين بلش فيهن البلد ولا لأ الست المتكررة يعني
+
+87
+00:07:48,680 --> 00:07:52,740
+الولد الثاني والثالث والرابع تحتاج لوقت أقصر شوية
+
+88
+00:07:52,740 --> 00:07:58,600
+يعني ممكن من ساعة إلى ساعة ونصف إذا عشان أقول هذه
+
+89
+00:07:58,600 --> 00:08:04,240
+حالة ولادة لازم الست يجيها تقلصات رحم اللي بنسميها
+
+90
+00:08:04,240 --> 00:08:10,720
+الطلق كل دقيقتين مرة وتستمر لمدة 45 ثانية طبعا
+
+91
+00:08:10,720 --> 00:08:18,080
+معلومة في نادر جدا من الستات بتحسش بالطلق سمعتوا
+
+92
+00:08:18,080 --> 00:08:26,320
+قصة زي هيك نادر جدا ستات بتحس بس بالبطن بشد وبتكون
+
+93
+00:08:26,320 --> 00:08:31,020
+هذه إيه كل عملية الطلقة أنت بتحسش بقلم نادر جدا
+
+94
+00:08:31,020 --> 00:08:37,500
+فبمعنى أن الست كل دقيقتين بيجي طلقة بتستمر 45
+
+95
+00:08:37,500 --> 00:08:40,900
+دقيقة البكرية في خلال ساعتين بدت لها إن شاء الله
+
+96
+00:08:40,900 --> 00:08:45,640
+المتكررة في خلال فترة أقصر شوية من ساعتين ففي
+
+97
+00:08:45,640 --> 00:08:49,120
+الساعتين أنا بعمل كل لازم ذاك اليوم ما شفت إلا
+
+98
+00:08:50,990 --> 00:08:54,930
+جارنا أجاني، بيقول لي يا عم أبو العبد، بيقول لي نعم،
+
+99
+00:08:54,930 --> 00:08:57,430
+جالي يا أخوي المرة صرت موديها تلت أربع مرات على
+
+100
+00:08:57,430 --> 00:09:01,470
+المستشفى وبيفحصوها الدكاترة وبيقول لي مش حالة
+
+101
+00:09:01,470 --> 00:09:04,810
+ولادة والحين مستلماني بتقول لي ودينا على المستشفى،
+
+102
+00:09:04,810 --> 00:09:08,830
+أنا صرت مستحمن حالي، طبعا الزلمة مزوج يعني وعنده
+
+103
+00:09:08,830 --> 00:09:12,630
+دينوكلامه صح وقلت له طب خليني جالي اللي بقول لك
+
+104
+00:09:12,630 --> 00:09:15,730
+أنا ما بديش يروح ويشوفوها الزلاب قلت له أنا ما بديش
+
+105
+00:09:15,730 --> 00:09:18,830
+أشوف ولا بدي أعمل ولا حاجة، ما بديها تجودك بالي على
+
+106
+00:09:18,830 --> 00:09:21,850
+الكرسي وأنا أشرب عندك وبقيت شاي وأطلع، جالي ده
+
+107
+00:09:21,850 --> 00:09:26,290
+هيك اتفضل فروحت، قاعدت، قلت له هي أم محمد اسمها،
+
+108
+00:09:26,290 --> 00:09:29,510
+اقعد يا عم محمد، طبعا هي والد جابل هيك، بس لها
+
+109
+00:09:29,510 --> 00:09:34,070
+فترة طويلة، هتقولي إنها نسيت يعني كيف حكيت الولادة
+
+110
+00:09:34,580 --> 00:09:38,140
+فقعدت أم محمد قلت لي يا أم محمد كل ما تيجي الطلق أو
+
+111
+00:09:38,140 --> 00:09:42,120
+تيجي التقلصات الرحم قولي أجت وبس فقعدنا شوية اللي
+
+112
+00:09:42,120 --> 00:09:47,740
+هجأت أجت بعتوا بنتهم عملت شاي وشربنا الشاي وخلص
+
+113
+00:09:47,740 --> 00:09:53,180
+الشاي وكان في موجز أخبار سمعنا موجز أخبار وما فيش
+
+114
+00:09:53,180 --> 00:09:56,000
+طلق قلت لي يا عم مش حالة طويلة قلت لي خلاص بارك الله
+
+115
+00:09:56,000 --> 00:09:59,280
+فيك ويخلف عليك وفعلا بعد بيجي حوالي يمكن سبوع أو
+
+116
+00:09:59,280 --> 00:10:04,240
+أشر أيام لما راحت أم محمد على المستشفى إيش ولدت في
+
+117
+00:10:04,240 --> 00:10:10,420
+المستشفى برضه ابن أجاني يابا المرأة بطنها بشد و
+
+118
+00:10:10,420 --> 00:10:14,920
+كأنه طبعا بكرية كأنه عندها طلق قلت له الطلق خليك
+
+119
+00:10:14,920 --> 00:10:19,060
+تجيبها قال حق جابها قلت له لا قعد يا ابنك قعدت كل ما
+
+120
+00:10:19,060 --> 00:10:22,780
+تيجي طلقة يا عم رفع يدك أو قولي لأ أجيب أول طلقة و
+
+121
+00:10:22,780 --> 00:10:25,500
+ثاني طلقة ثالث طلقة ما فيش بين الطلقة والثاني إلا
+
+122
+00:10:25,500 --> 00:10:28,660
+حوالي دي أكثر ونص أو ثلاثة حالة ولادة يا ابني
+
+123
+00:10:28,660 --> 00:10:31,580
+ركبنا السيارة في الليل وجبناها على الخدمة هنا
+
+124
+00:10:31,580 --> 00:10:36,730
+لعند تقريبا ساعة زمان كانت اللي هي والدة وعطوها
+
+125
+00:10:36,730 --> 00:10:42,250
+الجنين المولود وبعد ساعة ما استنينا عندهم روحنا 
+
+126
+00:10:42,250 --> 00:10:47,550
+مع طفلنا فإيش اللي كان بي ... بيخليني أقول إنه هذه
+
+127
+00:10:47,550 --> 00:10:52,830
+حالة ولادة إنه الـ set بيجيها تقلصات كل دقيقتين مرة
+
+128
+00:10:52,830 --> 00:10:58,220
+و بتستمر دقيقة إلا ربع، منفعش كل دقيقتين و ثواني تلت
+
+129
+00:10:58,220 --> 00:11:02,580
+أربع ثواني و خمس عشرة بظبطش، لازم تقعد مش أقل من
+
+130
+00:11:02,580 --> 00:11:08,120
+دقيقة إلا ربع كمان، لو أنت طلعت على الـ set بتلاقي حين
+
+131
+00:11:08,120 --> 00:11:13,300
+تطلق في حالة الولادة جزء من الرأس بيبين حوالي خمس
+
+132
+00:11:13,300 --> 00:11:18,460
+سنتيمتر من دائرة الرأس بيبين، لأن أول ما بنزل الأصل في
+
+133
+00:11:18,460 --> 00:11:23,320
+الولادة الطبيعية، أول ما بنزل من الجنين الرأس وأكبر
+
+134
+00:11:23,320 --> 00:11:27,830
+ما بنزل من الجنين، الرأس هو أصعب ما بنزل من الجنين
+
+135
+00:11:27,830 --> 00:11:32,710
+مجرد من نزلة الرأس بتلاقي الباقي كله بنزل كأنك
+
+136
+00:11:32,710 --> 00:11:39,130
+بتصيب ... تصب نقطة من ثقة بأثقة، عادي بسيطة جدا جدا
+
+137
+00:11:39,130 --> 00:11:43,350
+يعني القصة كلها بس في الرأس، إذا نزلت الرأس كل ما
+
+138
+00:11:43,350 --> 00:11:48,430
+بعدها أيسر منها، وطبعا الرأس سبحان الله ... ربنا زي
+
+139
+00:11:48,430 --> 00:11:52,750
+ما قلنالكم يسر الطريق، ذاكرين لما حكينا عن المفاصل
+
+140
+00:11:52,750 --> 00:11:59,370
+الثابتة؟ قلنا وين في مفاصل ثابتة في الإنسان؟ نسيت،
+
+141
+00:11:59,370 --> 00:12:04,890
+في الرأس، قلناش فيديتها، مين ذاكرة؟ بتلزم حين أنت
+
+142
+00:12:04,890 --> 00:12:08,930
+الميدالي، المفاصل بين الرأس بتلاقي الرأس ضغطت حالها
+
+143
+00:12:08,930 --> 00:12:13,290
+شوية مع الميلاد، وبعد هيك خلاص بتلزمش المفاصل هذه
+
+144
+00:12:13,290 --> 00:12:19,230
+فبتثبت، بتسكر، عشان هيك النفوخ تبع الطفل بعد سن زمان
+
+145
+00:12:19,230 --> 00:12:23,230
+بيكون مالوش وجود، مسكر خالص و الرأس صارت شجفة
+
+146
+00:12:23,230 --> 00:12:28,990
+واحدة، وقت الميلاد لزمته، بعد هيك بتكون انتهت، فعملية
+
+147
+00:12:28,990 --> 00:12:33,090
+الولادة يعني بتكون طبيعية و بلاد وشيكة، إذا كانت
+
+148
+00:12:33,090 --> 00:12:38,010
+الست بيجيها تقلصات في الرحم كل دقيقتين مرة، و التقلص
+
+149
+00:12:38,010 --> 00:12:42,190
+بيقعد مش أجل من خمسة و أربعين دقيقة، هيك بتكون
+
+150
+00:12:42,190 --> 00:12:47,610
+البكرية تلد في خلال ساعتين، المتكررة يعني أكتر من
+
+151
+00:12:47,610 --> 00:12:52,050
+مرة، ممكن تلد في وقت أقل في حدود ساعة، ساعة ونص،
+
+152
+00:12:52,050 --> 00:12:57,310
+ساعة وربع بتلد، و زي ما قلنا بتطلعي بتلاقي جزء
+
+153
+00:12:57,310 --> 00:13:01,650
+من الرأس حين الشد أو حين الطلق بيبين حوالي خمس
+
+154
+00:13:01,650 --> 00:13:06,830
+سنتيمتر من فروة الرأس بتبين، هذه بتكون حالة ولادة
+
+155
+00:13:15,170 --> 00:13:19,590
+و زي ما قلنا يعني في كل البلد مافيش مستشفيات ولادي
+
+156
+00:13:19,590 --> 00:13:24,150
+ولا عيادات ولادي إلا لابن الأدمين مظبوط؟ ليش؟ لأن
+
+157
+00:13:24,150 --> 00:13:30,270
+الله سبحانه وتعالى تكفل في هذه العملية، و الأصل أن
+
+158
+00:13:30,270 --> 00:13:37,250
+هذا عملية طبيعية مافيش فيها مشاكل طبعا
+
+159
+00:13:37,250 --> 00:13:40,210
+الولادة الطبيعية، كونها الولادة بتصير، الحمل بتصير
+
+160
+00:13:40,210 --> 00:13:45,490
+طبيعي، و الولد بتصير طبيعي، لا تمنع أن أنت كست تروحي
+
+161
+00:13:45,490 --> 00:13:50,070
+تعمل متابعة من أول يوم في الحمل، عارفين إن اليوم
+
+162
+00:13:50,070 --> 00:13:54,530
+بدوا يتابعوا الستات اللي نفسها تحمل قبل ما تحمل
+
+163
+00:13:54,530 --> 00:13:58,510
+يعني أيام زمان أذكر بقى في الوكالة مثلا مدام الست
+
+164
+00:13:58,510 --> 00:14:03,470
+ما تحركش الجنين ما يقبلوهاش زمان، الله الحين أول ما
+
+165
+00:14:03,470 --> 00:14:07,550
+تحمل بيبدوا يعطوها folic acid، مش هيك، folic acid
+
+166
+00:14:07,550 --> 00:14:15,100
+هذا و حديد، لأ، الـ folic acid قبل الحديد، بعدين الـ folic
+
+167
+00:14:15,100 --> 00:14:20,320
+acid هذا يقال إنه بيمنع حدوث تشوهات في الحمل، ففي
+
+168
+00:14:20,320 --> 00:14:25,120
+ناس بيعطوه حتى قبل الحمل، حتى إذا فكرت الواحد يتحمل
+
+169
+00:14:25,650 --> 00:14:28,490
+ماخدين bad cocaine بيودوا يعطوها folic acid عشان
+
+170
+00:14:28,490 --> 00:14:32,290
+ييجي الجنين من أول عمره يلاقي الـ folic acid، وطبعا
+
+171
+00:14:32,290 --> 00:14:38,210
+بتسهيل المولى بيمنع عملية إيش، التشوه في الجنين، بعد
+
+172
+00:14:38,210 --> 00:14:42,070
+فترة من الزمن، بتهيألي بعد الشهر التالت أو الرابع
+
+173
+00:14:42,070 --> 00:14:47,110
+بيصير folic acid مع حديد، بعد أربع شهور بيصير folic
+
+174
+00:14:47,110 --> 00:14:51,970
+acid مع حديد، ليش؟ لأنه الست بتصير بدها تعاطي من
+
+175
+00:14:51,970 --> 00:14:56,970
+دمها لمين؟ للجنين، و الجنين بيصير بده دم، فبنزود
+
+176
+00:14:56,970 --> 00:15:01,370
+عملية الدم للأم، في حين أن في كثير من الستات للأسف
+
+177
+00:15:01,370 --> 00:15:08,130
+بتتقبلوش، بتقولك أنا بحس كإني باكل صدر، فممكن سمعتوا
+
+178
+00:15:08,130 --> 00:15:14,850
+حاجات زي هي، في بعض الستات بتتجبلوش خالص يعني إلا
+
+179
+00:15:14,850 --> 00:15:19,390
+أنه طبعا يعني أنت جنوع من الحديد مع الـ folic acid
+
+180
+00:15:19,390 --> 00:15:24,790
+يعني ثئد خاص من السيدالية اللي طعم يعني مجبول أكتر
+
+181
+00:15:24,790 --> 00:15:35,510
+من اللي بنوزعه في الوكيلة زي
+
+182
+00:15:35,510 --> 00:15:43,110
+ما قلنا يعني أنهم ممكن الست يعني يسيرلها فجأة عملية
+
+183
+00:15:43,110 --> 00:15:51,630
+ولادة، يعني أنا عندي بنت جابت أول طفل يعني نشة
+
+184
+00:15:51,630 --> 00:15:56,870
+فريقنا، يمكن كان عمره أقل من سبع شهور وولد، يمكن
+
+185
+00:15:56,870 --> 00:15:59,530
+أقل من كيلو، بس ما شاء الله عنها الحين صار شاطر و
+
+186
+00:15:59,530 --> 00:16:06,340
+ذكى، فقلت يا جماعة بدنا نعملها غرزة، جال و بلزمهاش
+
+187
+00:16:06,340 --> 00:16:10,860
+طبعا كان راجل مع دكتوراه في النساء و الولادة، فلمّا
+
+188
+00:16:10,860 --> 00:16:18,430
+حملت الحمل الثاني في آخر السبع شهور، بدت اللي في
+
+189
+00:16:18,430 --> 00:16:23,370
+بطنها بده ينزل، فبدينا نتعامل معها، و زي ما بيقولوا
+
+190
+00:16:23,370 --> 00:16:29,850
+نحمل فيها تحميل أو نصبر فيها تصبير، إلى إنه فطت
+
+191
+00:16:29,850 --> 00:16:34,350
+الثامن و خشت في التاسع، ماخدين بالكوا كيف؟ طبعا مسكت
+
+192
+00:16:34,350 --> 00:16:38,430
+الدكتور و قولتله يا دكتور صار هيك هيك، قال أنا خلاص
+
+193
+00:16:38,430 --> 00:16:42,730
+اسف، مجرد متحمل المرة الجاية تعاليلي و أنا بعملك
+
+194
+00:16:42,730 --> 00:16:47,330
+الغرزة، بعمل غرزة عشان يكون فيه رخاوة في عنق الرحم
+
+195
+00:16:47,330 --> 00:16:52,130
+فبتحملش تجل الجنين بعد سبع شهور، ست شهور، فبتحملش
+
+196
+00:16:52,130 --> 00:16:57,590
+فبنزل، وهذا يعني سبب من أسباب اللي هي الولادة
+
+197
+00:16:57,590 --> 00:17:03,530
+المبكرة أو سقوط الجنين حتى يعني، فمعناته
+
+198
+00:17:03,530 --> 00:17:10,110
+الولادة قد تحدث قبل معادة، و قد تحدث في معادة، غالبا
+
+199
+00:17:10,110 --> 00:17:16,740
+معادة الولادة الست بتحبل متين و تمانين يوم، عرفت هذا
+
+200
+00:17:16,740 --> 00:17:23,340
+الحكي، الحمل الطبيعي عشر شهور قمرية تقريبا، اللي هي
+
+201
+00:17:23,340 --> 00:17:31,280
+متين اه، متين و تمانين يوم، بمعنى تسع شهور ميلادي
+
+202
+00:17:31,280 --> 00:17:36,360
+ماشي كيف؟ بيحسبوها، بيحسبوها طبعا و بيعطوكي في
+
+203
+00:17:36,360 --> 00:17:41,140
+العيادة تاريخ المتوقع للولادة، مش هيك بعملوها
+
+204
+00:17:42,160 --> 00:17:45,480
+التاريخ المتوقع للولادة يا أخوات الماجدات، معظم
+
+205
+00:17:45,480 --> 00:17:51,820
+الستات بتبتدي في هذا التاريخ، إلا إنه تعتبر الولادة
+
+206
+00:17:51,820 --> 00:17:57,700
+طبيعية جدا إذا ولدت الست قبل 15 يوم من التاريخ
+
+207
+00:17:57,700 --> 00:18:02,360
+المتوقع، و تعتبر الولادة طبيعية جدا إذا ولدت الست
+
+208
+00:18:02,360 --> 00:18:08,340
+بعد 15 يوم من التاريخ المتوقع، ماشي؟ طبعا في ستات
+
+209
+00:18:08,340 --> 00:18:12,540
+بتغلط في وقت إيش انقطعت عنها ... انقطعت الحيض عنها
+
+210
+00:18:12,540 --> 00:18:16,860
+أو مثلا تبقاش حسبت كويس، فبتقول تاريخ بطلع تاريخ مش
+
+211
+00:18:16,860 --> 00:18:21,020
+مظبوط، إلا إنه في الوضع الطبيعي خمس عشر يوم قبل
+
+212
+00:18:21,020 --> 00:18:25,640
+التاريخ يعتبر ولادة طبيعية، كمان خمس عشر يوم بعد
+
+213
+00:18:25,640 --> 00:18:30,100
+التاريخ يعتبر إيش، ولادة طبيعية
+
+214
+00:18:41,850 --> 00:18:49,470
+كيف بتتم عملية الولادة؟ كيف بدنا نولد السيد؟ عشان
+
+215
+00:18:49,470 --> 00:18:54,630
+نولد السيد طبعا يعني مش ... مش سهل إنه تلد السيد
+
+216
+00:18:54,630 --> 00:18:58,930
+وهي لابسة بنطلون كبير، مظبوط ولا لأ؟ لازم تكون
+
+217
+00:18:58,930 --> 00:19:05,270
+لابسة ... ملابس فاضية، ومش سهل نولد السيد وهي واقفة،
+
+218
+00:19:05,270 --> 00:19:09,580
+لازم تكون ممددة على مكان مريح، ما شاء الله هيقنى ستات
+
+219
+00:19:09,580 --> 00:19:15,940
+والدات و أطفالهم معاهم، لازم يكون شكل المنطقة مرتاح
+
+220
+00:19:15,940 --> 00:19:20,020
+و كويس، كمان يكون مزوي، لأنه صدقوا الست إذا كانت يعني
+
+221
+00:19:20,020 --> 00:19:24,780
+زي ما أقوله خجلانة بتأثر على الطلج، بتلاقي الطلج
+
+222
+00:19:24,780 --> 00:19:29,060
+ممكن يتوقف من عملية الخجل لأنه وضع نفسي، فبدأ يكون
+
+223
+00:19:29,060 --> 00:19:33,080
+حاططها في مكان مزوي بعيد عن الناس اللي مالهاش خاص
+
+224
+00:19:33,080 --> 00:19:39,760
+وطبعا ممكن يكون على طاولة، ممكن يكون ع سرير، ممكن
+
+225
+00:19:39,760 --> 00:19:43,400
+يكون على الأرض، بس لما تكون ع طاولة أو ع سرير أو
+
+226
+00:19:43,400 --> 00:19:49,320
+على الأرض، لازم تكون المادة دي على شيء صلب، بنحط
+
+227
+00:19:49,320 --> 00:19:53,380
+تحتيها يا خواتي نايلون، لأن الست بنزل منها إفرازات
+
+228
+00:19:53,380 --> 00:19:57,420
+و بنزل منها دم و بنزل منها سوائل، كمان أنا لازم
+
+229
+00:19:57,420 --> 00:20:01,560
+أكون لابس جوانتي، لازم أكون عندي قطعة قماش نظيفة
+
+230
+00:20:01,560 --> 00:20:07,130
+أستقبل فيها المولود، لأن المولود بنزل مليان شحمة
+
+231
+00:20:07,130 --> 00:20:15,870
+بنزل مليان مادة بيضة ... مليان مادة بيضة ... هذه
+
+232
+00:20:15,870 --> 00:20:20,930
+المادة البيضة كأنها سمنة يعني بكل بساطة، حصل إنه و
+
+233
+00:20:20,930 --> 00:20:26,030
+هم حاملين المولود بين إيدهم، وجأ عارفين إيش معناه
+
+234
+00:20:26,030 --> 00:20:29,750
+وجأ؟ خربت دياره، بجل عمر أو مات، مليان زي السمنة هذه
+
+235
+00:20:29,750 --> 00:20:37,700
+المادة سبحان الله العظيم، مهم جدا للطفل، إلها عدة
+
+236
+00:20:37,700 --> 00:20:43,480
+حاجات، أول حاجة كل المضادات الحيواني اللي في الدنيا
+
+237
+00:20:43,480 --> 00:20:47,920
+بتلاقيها فيه، الحاجة الثانية بتسهل عملية الولادة زي
+
+238
+00:20:47,920 --> 00:20:53,320
+التشحيم، لما تكون مشحم المنطقة عندك خاتم في أصباعك
+
+239
+00:20:53,320 --> 00:20:58,260
+مش راضي يطلع، بتحط سيرج أو زيت أو حاجة أو سمنة
+
+240
+00:21:01,620 --> 00:21:07,390
+فتلاقيه يطلع، هذا التشحيم برضه للجنين بعمل زي هيك
+
+241
+00:21:07,390 --> 00:21:11,650
+شغلة، بيصير أيصر ل ... و زي ما قلنا فيه كل المضادات
+
+242
+00:21:11,650 --> 00:21:17,670
+الحيوية و بيحمل الجنين من اللي هي تغيرات الجسم
+
+243
+00:21:17,670 --> 00:21:24,210
+الخارجية، كنا زمان أذكر مجرد ميلاد الطفل ناخده و
+
+244
+00:21:24,210 --> 00:21:30,110
+نحمه، اليوم اكتشفوا إنه ممنوع، أقل حاجة لازم يقعد
+
+245
+00:21:30,110 --> 00:21:34,250
+هذا 24 ساعة على جسمه، و لو كان 48 ساعة يكون أفضل
+
+246
+00:21:34,250 --> 00:21:39,550
+ليش؟ لأن هذا بيحميه من كل الجو و غبره من كل
+
+247
+00:21:39,550 --> 00:21:44,530
+المشاكل اللي برا، بيحمي هذا اللي هو المادة اللي
+
+248
+00:21:44,530 --> 00:21:48,730
+موجودة على جسمه، فإيه و يغسلوا الطفل قبل 48 ساعة
+
+250
+00:21:48,730 --> 00:21:52,230
+كان
+
+251
+00:21:52,230 --> 00:22:03,090
+زمان أنا بقول لك من أول ميلاد الأصل لأ لأنه هذا تمسّيح
+
+252
+00:22:03,090 --> 00:22:08,210
+غير التغسيل، لما تمسّحيه بظل أثار المادة موجود وع
+
+253
+00:22:08,210 --> 00:22:12,580
+رأسه بيبقى موجود معناته مش محمّم، واخد بالك؟ لو محمّم
+
+254
+00:22:12,580 --> 00:22:16,320
+كان محمّم من رأسه، فبمعنى أنه خلّي هذه المادة على
+
+255
+00:22:16,320 --> 00:22:21,040
+جسم الإنسان لأن هذه فيها مضادات حيوية ممتازة جدا
+
+256
+00:22:21,040 --> 00:22:26,800
+وبتساعد الجسم الصغير على أنه يعيش حياته الصحيحة
+
+257
+00:22:26,800 --> 00:22:32,000
+ويتأقلم مع الجو، هو بقى في جو ممتاز جداً، تأقلم عليه
+
+258
+00:22:32,000 --> 00:22:35,760
+معقّم ونظيف وطاهر، حين أتى في جو كله مايكروبات
+
+259
+00:22:36,290 --> 00:22:40,650
+مظبوط؟ لا طاهر ولا مطهّر، فبناء عليه المادة هذه
+
+260
+00:22:40,650 --> 00:22:47,490
+بتخلي جسمه يتأقلم على هيك، في بعض الستات يعني
+
+261
+00:22:47,490 --> 00:22:51,110
+بتلاقيها
+
+262
+00:22:51,110 --> 00:22:55,830
+يوم من الأيام واحدة بعرفها كويس ماشية على درجة
+
+263
+00:22:55,830 --> 00:23:00,480
+اندجمات وجعات اللي هي على المطرح، بدها تبدع، ممكن أي
+
+264
+00:23:00,480 --> 00:23:06,020
+إصابة أو أي صدمة أو أي هواية أو أي نتع حاجة أو إش
+
+265
+00:23:06,020 --> 00:23:11,060
+زي هي يخلّي الصت إيش ابتلد في غير معادة، وهذه بنظوم
+
+266
+00:23:11,060 --> 00:23:19,100
+نسميها إيش؟ نعم، بنسميها حالة ولادة طارئة، طبعاً زي ما
+
+267
+00:23:19,100 --> 00:23:25,720
+قلنا فيه ستات ابتلد طبعاً نادر جداً بدون ما يسير أي
+
+268
+00:23:25,720 --> 00:23:31,430
+نوع من الطريق أو الحزق، وهذه طبعاً بتكون عارفة حالها
+
+269
+00:23:31,430 --> 00:23:38,670
+يعني قبل هيك كمان يعني في ستات بتلاقيها مجرد ما 
+جيها الطلق بتصير إسراخ هيجيه بالتاية، وفي ستات
+
+270
+00:23:38,670 --> 00:23:42,530
+بتبقى تلد وشديد الألم وهي صابرة، طبعاً هذا يعني في
+
+271
+00:23:42,530 --> 00:23:47,470
+مدى التحمل ومدى صبر الست، إنما زي ما قلنا عشان
+
+272
+00:23:47,470 --> 00:23:53,510
+تقولي هذه عملية ولادة لازم يكون إيش؟ في توسّع وفي
+
+273
+00:23:53,510 --> 00:24:00,720
+تقلّصات، راحة من كل دقيقتين تستمر لمدة 45 ثانية ولو
+
+274
+00:24:00,720 --> 00:24:07,400
+اطلعتي بتشوفي جزء من رأس الجنين، هذا يعني أنه
+
+275
+00:24:07,400 --> 00:24:11,780
+الولادة وشيكا عشان تلد الست من مددها سواء على
+
+276
+00:24:11,780 --> 00:24:21,360
+طاولة طويلة، على سرير، طبعاً على الطاولة يعني إذا كان
+
+277
+00:24:21,360 --> 00:24:27,230
+الطاولة أكثر من هيك ممكن أحط كمان كرسيين أتريح
+
+278
+00:24:27,230 --> 00:24:31,390
+أجريهم عليه، وبنجي في جبالها وبنبدأ نستقبل اللي
+
+279
+00:24:31,390 --> 00:24:36,750
+هو إيش؟ الجنين طبعاً إيه وإيه، تقولي للست قبل ما
+
+280
+00:24:36,750 --> 00:24:41,390
+تبدأ رأس الجنن الجنين يتبين، تقولي له أعزجيشدي لإن
+
+281
+00:24:41,390 --> 00:24:47,250
+الشد هذا هيرهقها ويتعبها، وحين ما بدياها تشد تبقى
+
+282
+00:24:47,250 --> 00:24:52,590
+استوت تبقى إيش؟ استوت لإن هي الآن يعني الحمل و
+
+283
+00:24:52,590 --> 00:24:59,210
+الولادة حاجة مش سهلة، حاجة متعبة جداً جداً وإرخاق و
+
+284
+00:24:59,210 --> 00:25:03,750
+زي ما بيقولوا بنزع الروح من روح، فلما أخليها تشد
+
+285
+00:25:03,750 --> 00:25:08,050
+تشد تشد ما بيصل وجد أقول لها ساعديني، اللي هي الست
+
+286
+00:25:08,050 --> 00:25:12,210
+إيش؟ ما فيش عندها أي نوع من القوة فبستنى عليها فقط
+
+287
+00:25:12,210 --> 00:25:16,400
+لما أنا احتاج إنّها تتشد، لما أتنام على الستة ع
+
+288
+00:25:16,400 --> 00:25:21,520
+ظهرها بتقعد اللي .. اللي بده يولدها جبالها وبنتظر
+
+289
+00:25:21,520 --> 00:25:27,260
+لما تطلع الرأس، لما تيجي الرأس تظهر، أول ما بظهر في
+
+290
+00:25:27,260 --> 00:25:33,380
+الولادة الطبيعية من الجسم الرأس، في خطورة قبل ظهور
+
+291
+00:25:33,380 --> 00:25:38,860
+الرأس، الخطورة قبل ظهور الرأس أنه كيس الماء اللي
+
+292
+00:25:38,860 --> 00:25:44,040
+حوالين الجنين ينبعد أو ينفجر، إذا انفجر كيس الماء
+
+293
+00:25:44,040 --> 00:25:49,560
+حوالين الست، الأصل إنّها تنام على ظهرها وما تتحركش
+
+294
+00:25:49,560 --> 00:25:55,620
+ليش؟ لأن الجنين مع الحبل السري عايم وين؟ في وسط
+
+295
+00:25:55,620 --> 00:26:03,380
+الماء، فمعلق، ابعد عن بعضه، وإن راحت الماء شو بيصير؟
+
+296
+00:26:03,380 --> 00:26:09,140
+بيصير احتمال كبير إنّ الحبل السري ينطعج، يعني ثاني و
+
+297
+00:26:09,140 --> 00:26:14,640
+يسكل ونسكر الحبل السري، معناته الطفل بطل ياخد
+
+298
+00:26:14,640 --> 00:26:19,140
+Oxygen وبطل ياخد تغذية، فممكن يخترق وهو في داخل
+
+299
+00:26:19,140 --> 00:26:24,440
+أمه، فبدناش تتحرك، كمان مش بس بدّيش هي تتحرك بدّيها
+
+300
+00:26:24,440 --> 00:26:29,280
+تنام نوم مجلوب، كيف يعني مجلوب؟ يعني تيجي بالشكل
+
+301
+00:26:29,280 --> 00:26:36,180
+هذا
+
+302
+00:26:36,180 --> 00:26:36,560
+زي هيك، ليش؟ عشان ما يصيرش ضغط على منطقة الولادة
+
+303
+00:26:42,570 --> 00:26:48,390
+والظلّ الجنين راجع لوراه إلى أن تصل إلى أقرب مركز
+
+304
+00:26:48,390 --> 00:26:53,610
+طبي، طبعاً إذا هو حصلت الولادة أو حصلت يعني في مكان
+
+305
+00:26:53,610 --> 00:27:00,480
+بدها ستر وبدها ت .. تحطيها في مكان خاص، ما تخليش
+
+306
+00:27:00,480 --> 00:27:07,700
+يعني زي ما قلنا ستات ممكن لو ما كانتش في منطقة خاصة
+
+307
+00:27:07,700 --> 00:27:12,360
+يتوقف عندها الطلق وتخجلماتقدرش يعني تد معاكي فهذا
+
+308
+00:27:12,360 --> 00:27:18,170
+بيسوي مشكلة، بدك تكون جاهزة بما تحتاج إليه اللي هي
+
+309
+00:27:18,170 --> 00:27:24,510
+قصة الولادة، إيش أنا بالزمن في الولادة؟ بالزمن قطعة
+
+310
+00:27:24,510 --> 00:27:28,910
+قماش أستقبل فيها الجنين، بالزمن مربط، اليوم في ملاقط
+
+311
+00:27:28,910 --> 00:27:35,290
+عرفتهم؟ في ملاقط لصرة، فيش ملجط بدي خيط عريض أو قطعة
+
+312
+00:27:35,290 --> 00:27:44,080
+يعني زي رباط الجزمة يكون نظيف معقّم ماشي عشان أربط
+
+313
+00:27:44,080 --> 00:27:50,980
+فيه إيش؟ الحبل السري، وبدّي أداة أقطع فيها الحبل
+
+314
+00:27:50,980 --> 00:27:56,120
+السري، وبدّي ملابس للطفل وملابس للأم، حد ما تلقى
+
+315
+00:27:56,120 --> 00:28:00,820
+للواحدة كينجال وين أهل فلانة، أعطونا ملابس الطفل و
+
+316
+00:28:00,820 --> 00:28:08,250
+غالباً بندخل الملابس معاه يعني ممكن ماشي فبنا ملابس
+
+317
+00:28:08,250 --> 00:28:12,810
+للطفل وبنا ملابس لأمه ونستقبل الطفل طبعاً بقطعة
+
+318
+00:28:12,810 --> 00:28:18,810
+قماش نظيفة عشان إنه الطفل زي ما قولنا بينزل مليان
+
+319
+00:28:18,810 --> 00:28:24,930
+بمادة كالشحمة، هذه المادة كالشحمة بتلاقي اللي بتجع
+
+320
+00:28:24,930 --> 00:28:29,310
+من الإيد لما سكتيه بيجع، الآن قعدنا نولد الست
+
+321
+00:28:29,310 --> 00:28:33,850
+قعدنا نولد الست، الست نايم على ظهرها، ببدأ تقلّصات
+
+322
+00:28:40,660 --> 00:28:45,220
+الرحم، بتبدأ جزء من الرأس يبين، بيجي دفقة أو تقلّص
+
+323
+00:28:45,220 --> 00:28:53,040
+شديد، بتطلع الرأس، أول ما بيطلع في الوضع الطبيعي من
+
+324
+00:28:53,040 --> 00:28:57,360
+الرأس، مؤخرة الرأس، مؤخرة الرأس، إذا عمرك انشفت ست
+
+325
+00:28:57,360 --> 00:29:02,660
+بكرية ولدها، بتلاقي ابنها نافق رأسه شوية من هنا،
+
+326
+00:29:02,660 --> 00:29:07,680
+غير النفوخ هذه، هذه المنطقة وارمة وارمة، ليش وارمة؟
+
+327
+00:29:07,680 --> 00:29:14,120
+لأن الضغط كله عليها فانجرحت فصار فيه دم شوية طبعاً
+
+328
+00:29:14,120 --> 00:29:18,020
+هذا بعد أسبوعين، زمان شهر زمان بيروح، بمعنى أنه أول
+
+329
+00:29:18,020 --> 00:29:22,340
+ما بينزل من الرأس اللي هي المنطقة الجزء العلوي من
+
+330
+00:29:22,340 --> 00:29:27,280
+الرأس، وهذه في الطفل أصغر منطقة في الرأس بتطلع في
+
+331
+00:29:27,280 --> 00:29:32,940
+الطفل وهو داير وجهه وإيه كده قاعد ليه، بتلاقي
+
+332
+00:29:32,940 --> 00:29:36,000
+منطقة رأسه جاية بالشكل هذا
+
+333
+00:29:36,000 --> 00:29:38,880
+صح؟ فهذه بتلاقيها إيش؟ أصغر منطقة ممكن تنزل، بعد
+
+334
+00:29:45,610 --> 00:29:50,690
+هيك بتبدأ تتربع الرأس وتدور ويتغير شكلها تكبر
+
+335
+00:29:50,690 --> 00:29:55,230
+وتصير بوضعها، الآن نزلت الرأس، إيش الخطورة عند
+
+336
+00:29:55,230 --> 00:30:00,170
+نزول الرأس؟ الخطورة الأولى قلنا إنه ينفجر كيس
+
+337
+00:30:00,170 --> 00:30:04,950
+الماء، إذا انفجر كيس الماء بدأت نام على ظهرها أو
+
+338
+00:30:04,950 --> 00:30:08,350
+ممنوع تمشي، في البيت، انفجر كيس الماء، ممكن أنامها
+
+339
+00:30:08,350 --> 00:30:14,900
+برضه بالطريقة العكسية عشان ما يصيرش دفع هالوين،
+
+340
+00:30:14,900 --> 00:30:19,020
+ليش؟ لأن الحبل السري عايم في الماء، راحت الماء،
+
+341
+00:30:19,020 --> 00:30:25,480
+ممكن ينتني الحبل السري وتيجي الرأس أو جزء من
+
+342
+00:30:25,480 --> 00:30:29,620
+الجسم يضغط عليه، انتنى وصار ضغط عليه من الجسم،
+
+343
+00:30:29,620 --> 00:30:34,200
+إيش صار؟ انقطع الـ Oxygen وانقطع التغذية عن الجنين
+
+344
+00:30:34,200 --> 00:30:40,080
+ممكن يخش في مشاكل فبدّيش أخليها تتحرك وبدّي أنام
+
+345
+00:30:40,080 --> 00:30:44,420
+بطريقة زي هيك، الآن في حالة الولادة قلنا بنقعد أمام
+
+346
+00:30:44,420 --> 00:30:49,400
+الست وبنستقبل الرأس بقطعة الأقنعة، إيش كمان
+
+347
+00:30:49,400 --> 00:30:53,820
+المشكلة الأخرى اللي ممكن تحصل إنّه لما تيجي الرأس
+
+348
+00:30:53,820 --> 00:30:57,100
+تطلع يطلع ملفوف عليها إيه الحد السري، الحبل السري
+
+349
+00:30:57,100 --> 00:31:02,600
+ملفوف على رجباه وموصول وين؟ في المشيمة
+
+350
+00:31:02,600 --> 00:31:08,660
+المشيمة موصول فيها الحبل السري، فلحين لو جينا جدنا
+
+351
+00:31:08,660 --> 00:31:12,220
+هينخنج الطفل وينقطع الحبل السري، خشينا في نزيف
+
+352
+00:31:12,220 --> 00:31:17,520
+وخشينا في خنج للطفل، فإذا لجيت .. لما تنزل رأس لجيت
+
+353
+00:31:17,520 --> 00:31:22,640
+ملفوف الحبل السري، بكل بساطة حط أصبعينك وحاول
+
+354
+00:31:22,640 --> 00:31:26,660
+اتحرري الحبل السري عن الرأس، يعني تبعد الحبل السري
+
+355
+00:31:26,660 --> 00:31:31,580
+عن الرأس، فبيصير الرأس إيش؟ لحالها، وبعد هيك طبعاً مع
+
+356
+00:31:31,580 --> 00:31:35,620
+الطلجة الثانية بتحط إيديكي تحت اكتاف الجنين تطلع
+
+357
+00:31:35,620 --> 00:31:40,320
+رجبته ورأسه، فببدأ دور مين؟ هالعين، الأكتاف أصغر من
+
+358
+00:31:40,320 --> 00:31:45,300
+الرأس، اكتاف الطفل أصغر من الرأس، وهذا الطفل اكتافه
+
+359
+00:31:45,300 --> 00:31:50,340
+أكبر، ليش كبير مش صغير ولا لأ؟ فحين مولود كده كان
+
+360
+00:31:50,340 --> 00:31:55,860
+اكتافه رأسه أكبر، ولا مش ذاكرة؟ قداش كانت رأسه؟ و
+
+361
+00:31:55,860 --> 00:32:05,890
+كتفه صغير جداً؟ بقى الآن طلعت الرأس فبدّي أسحب الجنين
+
+362
+00:32:05,890 --> 00:32:11,280
+بحط إصبعيني تحت قبطه وبكل بساطة بطلع زي ما بقوله
+
+363
+00:32:11,280 --> 00:32:17,640
+زي ما بتطلع نقطة الماء من الكاسة سهولة جداً بتطلع
+
+364
+00:32:17,640 --> 00:32:22,420
+الطفل، حين طلع الطفل معانا بيصير عندي الحبل السري
+
+365
+00:32:22,420 --> 00:32:26,620
+جزء في الطفل الشجف الثاني موجود وين؟ في داخل الأم
+
+366
+00:32:26,620 --> 00:32:31,360
+مربوطة في وين؟ في المشيمة أو الخلاصة
+
+367
+00:32:31,360 --> 00:32:34,740
+الشعر إيش بيقول؟ بيقول: ولدت كأمك يا ابن آدم باكياً،
+
+368
+00:32:37,600 --> 00:32:43,280
+أول ما يصل اصل إيش؟ يصرخ، احنا بنبقى قاعدين نستنى
+
+369
+00:32:43,280 --> 00:32:48,640
+في الست اللي بتولد جوا، مجرد ما نسمع صوت الصراخ
+
+370
+00:32:48,640 --> 00:32:52,540
+بنقول الحمد لله ولدت، مظبوط؟ بيبقى جوزك، بيبقى
+
+371
+00:32:52,540 --> 00:32:56,400
+أخوك، بيبقى عمك قاعدينش الحمد لله ولدت، لحد ما
+
+372
+00:32:56,400 --> 00:33:00,600
+تصرخ، فولدتك أمك يا ابن آدم باكياً والناس حولك
+
+373
+00:33:00,600 --> 00:33:06,060
+يضحكون بسرورة، فأعمل ليوم أن تكون إذا بكت الناس
+
+374
+00:33:06,060 --> 00:33:11,340
+حولك ضاحكاً مسروراً، أنا الحين مبسوط إنه .. وهو بعيط
+
+376
+00:33:16,520 --> 00:33:21,380
+و أنا متيقن أنه أجانب جديد بكرا بنبقى نعيط وهو
+
+377
+00:33:21,380 --> 00:33:25,780
+نايم أنه بده يروح فهو بده يكون يضحك مين اللي بيكون 
+
+378
+00:33:25,780 --> 00:33:31,480
+بضحك اللي بيكون شايف اللعيم اللي هو بده ينتقل إليه
+
+379
+00:33:31,970 --> 00:33:37,910
+فالآن الولد الأصل زي ما قلنا ينزل يبكي أو يصرخ
+
+380
+00:33:37,910 --> 00:33:45,650
+مجرد ما نزل الطفل بيصير انفصل عن أمه طبعا الستات
+
+381
+00:33:45,650 --> 00:33:49,410
+اللي ولده بعرفوا بتقعد الحبل السري يتحرك أبو
+
+382
+00:33:49,410 --> 00:33:55,110
+دقيقتين إذا لاحظتوها احنا فاضين نلاحظ وإنا معانا
+
+383
+00:33:55,110 --> 00:33:59,230
+خضار؟ مش فاضين، عندهم مهام بتلاقي الحد السري 
+
+384
+00:33:59,230 --> 00:34:01,690
+بوجوه اللي حوالي دقيقة أو دقيقتين يتحرك، إيش معناه
+
+385
+00:34:01,690 --> 00:34:06,390
+أنت بتتحرك؟ معناه ذلك يدعم بالأكسجين والتغذية
+
+386
+00:34:06,390 --> 00:34:12,710
+والدم من أمه، يعني يتغذى من مين؟ من أمه، بيبقى
+
+387
+00:34:12,710 --> 00:34:17,290
+يتغذى لازال ..
+
+388
+00:34:17,290 --> 00:34:25,010
+بيظل إيش؟ يتغذى من أمه بمعنى استني دقيقتين تلاتة
+
+389
+00:34:25,010 --> 00:34:30,350
+قبل أن تروح هذه الحركة بعد الولادة بتقصي بدقيقتين
+
+390
+00:34:30,350 --> 00:34:33,870
+تلاتة لما توقف الحركة لأن دي بتبقى بتتحرك وباخد 
+
+391
+00:34:33,870 --> 00:34:38,610
+دم وباخد أكسجين وباخد تغذية من مين؟ من الأم
+
+392
+00:34:38,610 --> 00:34:42,190
+استفيد هالدقيقتين يعني ليش أنت تحرمي؟ الآن طلع
+
+393
+00:34:42,190 --> 00:34:47,710
+الجنين خلصنا منه أنا بعمل مجرد متلة لقمة بحطه على
+
+394
+00:34:47,710 --> 00:34:54,080
+بطنها على صدرها هذه الحركة بتعمل حاجات حلوة إيش
+
+395
+00:34:54,080 --> 00:35:00,960
+بتعمل؟ بتخلي الأم تشعر أنه يعني فيه صلة بتخلي الأم
+
+396
+00:35:00,960 --> 00:35:06,540
+تشعر في صلة الحاجة الثانية، بتخليها ترده طبعا بعد
+
+397
+00:35:06,540 --> 00:35:10,400
+ما جُسّت السرة والربط وبتخليها ترده إرضاعا في
+
+398
+00:35:10,400 --> 00:35:15,400
+اليوم الأول والثاني والثالث بتجيبش حليب كثير لكن
+
+399
+00:35:15,400 --> 00:35:21,760
+إيش بتعمل؟ بتعمل تنشيط لخلايا الثدي اللي هي
+
+400
+00:35:21,760 --> 00:35:27,890
+الخلايا الحليبية بتخليها تسير تفرز الحليب الأهم
+
+401
+00:35:27,890 --> 00:35:33,710
+كمان أن الطفل بياخد كل المضادات الحيوية والتطعيمات
+
+402
+00:35:33,710 --> 00:35:38,230
+اللي في بطن أمه اللي في جسم أمه كل تطعماتك اللي في
+
+403
+00:35:38,230 --> 00:35:44,230
+جسمك بتنزل في حليب اللبأ نسميه حليب اللبأ أول تلت
+
+404
+00:35:44,230 --> 00:35:48,870
+أيام طبعا يعني بيجوا ناس يقولوا لك بالله إيش
+
+405
+00:35:48,870 --> 00:35:53,570
+الحليب اللي نسجيه للولد نعم أخوي حليب إيش ما هو مش
+
+406
+00:35:53,570 --> 00:35:59,960
+راضي ينزل حليب من أمه لأ راضي بكون أنا قولي لهم بعنف
+
+407
+00:35:59,960 --> 00:36:04,940
+يعني المفروض يرضع هذا الأمر لازم تقعد أقل حاجة ربع
+
+408
+00:36:04,940 --> 00:36:09,260
+ساعة على الثدي اليمين وربع ساعة على الثدي اليسار
+
+409
+00:36:09,260 --> 00:36:15,380
+في كل مرة وهي يتردد سواء رضع أو ما رضعش لازم يقعد
+
+410
+00:36:15,380 --> 00:36:19,380
+يمص ربع ساعة هذا المص إيش بيساوي أول حاجة بيصير
+
+411
+00:36:19,380 --> 00:36:24,880
+حنان تصير تحبي ويحبك كويس وتنبسط عليه وينبسط
+
+412
+00:36:24,880 --> 00:36:29,200
+عليك ويتعرف عليك وتتعرف عليه الحاجة الثانية بينشط
+
+413
+00:36:29,200 --> 00:36:35,480
+خلايا الحليب أول مرة اضطرتي بقى ينزل إشي درجة
+
+414
+00:36:35,480 --> 00:36:39,100
+صرتي بدك ترضعي حليب صداعي صح ولا ما حاولتيش
+
+415
+00:36:39,100 --> 00:36:45,620
+فكرتي؟ شوفتوا عاد؟ هذه طبعا من الأخطاء الشائعة هذه
+
+416
+00:36:45,620 --> 00:36:50,820
+من الأخطاء الشائعة ليش؟ لأن الطفل بتجلى وبنبسط
+
+417
+00:36:50,820 --> 00:36:55,680
+على الحليب الصناعي الحليب الصناعي بدوش مجهود لحاله
+
+418
+00:36:55,680 --> 00:36:59,380
+البز بيسوق .. بيكتب في تم الطفل دقيقتين تلاتة بيكون
+
+419
+00:36:59,380 --> 00:37:07,660
+صح بالجنيه الحاجة الثانية أكثر حلوة في سكر أكثر يا
+
+420
+00:37:07,660 --> 00:37:11,380
+أختي ما أنا قاعد بحكي أنت قاعد إيش بقول سمع إيش
+
+421
+00:37:11,380 --> 00:37:15,720
+قاعد بقول خليه يرضع ربع ساعة الثدي اليمين وربع
+
+422
+00:37:15,720 --> 00:37:23,560
+ساعة الثدي الشمال غلبك ازجيه مية مية اه مية عادية و
+
+423
+00:37:23,560 --> 00:37:27,600
+هو مولود صغير اه سجية كوباية مية ست يا الله يارحمها
+
+424
+00:37:27,600 --> 00:37:33,480
+بجيتها تحط نتفة سكر فضل ماشي سجية نتفة معلقة بسكر
+
+425
+00:37:33,480 --> 00:37:42,420
+فضل بسجية مش بضاعة مش بجنيه بالظبط
+
+426
+00:37:42,420 --> 00:37:47,920
+بالظبط هي يانسون كراوية مية دافية إيه اللي حكاية
+
+427
+00:37:47,920 --> 00:37:53,550
+أنك تجيب له حليب صناعي الحليب الصناعي مشكلة حلو وسهل
+
+428
+00:37:53,550 --> 00:37:58,330
+أنه يشربه فالمكره لما ييجي ينتع في صدر أمه بيقولك
+
+429
+00:37:58,330 --> 00:38:02,410
+بديش فما فيه واحد انبعث على بجهة أسهل ليه وعلى السريع
+
+430
+00:38:02,410 --> 00:38:06,030
+شفط وانبسط مش اللي عملوا فيا بدون ما يعقبوا ليه
+
+431
+00:38:06,030 --> 00:38:12,010
+بطلت ودينا بالكوا الصغير بنزل يفهم بنزل عنده حاجة
+
+432
+00:38:12,010 --> 00:38:19,490
+أنت بتعرفيهاش اللي هي خاصية الرضاعة، مظبوط؟ خاصية
+
+433
+00:38:19,490 --> 00:38:24,550
+الرضاعة اللي حطيتها فيه بطريقة مهمة جدا بيسكر تِمه
+
+434
+00:38:24,550 --> 00:38:30,470
+كويس وبعرف يشفط بيسكر تِمه جيد جيد يعني وبشفط
+
+435
+00:38:30,470 --> 00:38:35,990
+وما فيه هذه الخاصية طبعا هذه الخاصية سهل أنه يخسرها
+
+436
+00:38:35,990 --> 00:38:41,710
+سهل أنه يخسرها كيف بيخسرها ولد بنوديه على حضانة
+
+437
+00:38:41,710 --> 00:38:47,470
+مريض مثلا إذا الحضانة ما أدّته study أو أدّته
+
+438
+00:38:47,470 --> 00:38:51,970
+business صناعي يتعلم فيه المصر بيروح على study أمه
+
+439
+00:38:51,970 --> 00:38:57,930
+بعد يومين تلاتة بقارفش المصر خلاص يبقى انتهت و
+
+440
+00:38:57,930 --> 00:39:01,530
+المهم يعني أنه لازم يتعلم يستخدم تِمه لعملية
+
+441
+00:39:01,530 --> 00:39:07,280
+الشفط ماشي فعملية المص هذه مهمة جدا جدا بتعطي الولد
+
+442
+00:39:07,280 --> 00:39:11,420
+اللي هو المضادات الحيوية كلها اللي في الدنيا في
+
+443
+00:39:11,420 --> 00:39:16,300
+جسمك وبتعطيك كل تطعيماتك إيش في جسمك؟ تطعيمات
+
+444
+00:39:16,300 --> 00:39:21,550
+بياخدهم الطفل بواسطة اللي حليب اللبن الحاجة الثانية
+
+445
+00:39:21,550 --> 00:39:26,850
+حليب الله بيعته ولا بدك تشتري ولا بدك تغلي ولا
+
+446
+00:39:26,850 --> 00:39:31,850
+بدك تركزي ولا بدك تسخني وماشي مع عمر الطفل فلحين
+
+447
+00:39:31,850 --> 00:39:35,930
+يبدوا ياه خفيف .. يبدوا ياه خفيف ربنا بيعمله خفيف
+
+448
+00:39:35,930 --> 00:39:39,590
+بكرا بكبر بتقل الحليب تماخدين بالكوا كيف؟ فيعني
+
+449
+00:39:39,590 --> 00:39:43,670
+نعمة أنت بتحرميها .. بتحرميها عشان هيك يا حق
+
+450
+00:39:43,670 --> 00:39:47,890
+الطفل أنه يرضع من أمه الضاعة اللي هي ..
+
+451
+00:39:51,140 --> 00:39:57,640
+حنحكي في الموضوع .. لأ مش في الحالي بس .. لأ لأ
+
+452
+00:39:57,640 --> 00:40:03,960
+نحكي .. نحكي في الموضوع بعدين الآن
+
+453
+00:40:03,960 --> 00:40:09,780
+احنا وقتاش بننقل الأم إلى المستشفى أي جزء من
+
+454
+00:40:09,780 --> 00:40:13,460
+الطفل بنزل غير الرأس بتكون الولادة غير طبيعية
+
+455
+00:40:13,460 --> 00:40:22,540
+ماشي؟ ونزول الماء اللي في حوالي الطفل ما .. اللي
+
+456
+00:40:22,540 --> 00:40:27,100
+بيكون عِين في الطفل هذا يعني أن الست بده ننقلها إلى
+
+457
+00:40:27,100 --> 00:40:33,020
+المستشفى في عدة حاجات يعني تحتاج أن ننقل الست
+
+458
+00:40:33,020 --> 00:40:37,460
+فيها إلى المستشفى إذا مثلا الست أكلته هوايا مثلا و
+
+459
+00:40:37,460 --> 00:40:41,990
+أجت ولادة بسرعة هيك وصار نزيف حكي .. ذكي .. حكينا
+
+460
+00:40:41,990 --> 00:40:46,250
+أنه النزيف في الأشهر الأخيرة من الحمل يحتاج ع
+
+461
+00:40:46,250 --> 00:40:49,930
+السريع نقل إلى المستشفى، صار نزيف عند السيد فكل
+
+462
+00:40:49,930 --> 00:40:53,130
+هذه شغلات بننقل السيد فيها إلى المستشفى، بننقل
+
+463
+00:40:53,130 --> 00:40:56,010
+السيد فيها إلى المستشفى، ما تقول لهاش في الطريق
+
+464
+00:40:56,010 --> 00:41:01,440
+أحزج كمان ما تمنعيش الجنين إذا بده يلد أنه يلد،
+
+465
+00:41:01,440 --> 00:41:05,420
+ما يجدرش أنا أ sit في وسط السيارة نزلة الرأس، تقدر
+
+466
+00:41:05,420 --> 00:41:10,520
+تقولي لأ رجعيها أو ممنوعة تنزل؟ لأ فبكل بساطة
+
+467
+00:41:10,520 --> 00:41:16,860
+بتعامل إيش كأنها يعني في البيت وبتعامل بطريقة
+
+468
+00:41:20,030 --> 00:41:24,990
+احنا قلنا الحاجة المهمة كس .. كيس الماء ينفجر أو
+
+469
+00:41:24,990 --> 00:41:28,850
+الحبل السري بنزل، إذا الحبل السري نزل قبل الجنين
+
+470
+00:41:28,850 --> 00:41:33,790
+أو التف حواليه غسل جنين هذا طبعا لازم جراي جراي
+
+471
+00:41:33,790 --> 00:41:36,470
+ننقله إلى المستشفى
+
+472
+00:41:38,540 --> 00:41:42,840
+طبعا عشان ننقل السيد كنا بنخلي رجليها عند رأس ..
+
+473
+00:41:42,840 --> 00:41:47,760
+لرقب .. لصدرها عشان يخفف وبنخلي رأسها لتحت بحيث
+
+474
+00:41:47,760 --> 00:41:54,060
+أنه ما يصيرش ضغط وينزل الحبل السري وينخنق الجنين
+
+475
+00:41:54,060 --> 00:41:57,320
+الآن
+
+476
+00:41:57,320 --> 00:42:06,340
+اتفضلي لأ
+
+477
+00:42:11,600 --> 00:42:16,940
+لما يتبين الرأس عندما تظهر الرأس احنا دورنا كمولدين
+
+478
+00:42:16,940 --> 00:42:22,900
+أو كمسعفين أن نستقبل هذه الرأس بقطعة قماش نظيفة
+
+479
+00:42:22,900 --> 00:42:27,900
+لأن مجلنا بلاش يكون بدينا بيبقى لابس جوانتي إذا
+
+480
+00:42:27,900 --> 00:42:34,540
+أمكن أو لابس شيء يمنع هذا ويكون زي ما قلنا مليان
+
+481
+00:42:34,540 --> 00:42:42,190
+بمادة وقد يكون السلة مثلا ينزل جزء منها أو يكون
+
+482
+00:42:42,190 --> 00:42:49,050
+نازل جبل أحيانا بيقولوا بلد الولد بال bornos اسمها
+
+483
+00:42:49,050 --> 00:42:54,710
+تعجغلة ايك يعني كيس الماء ما انفجرش كيس الماء اللي
+
+484
+00:42:54,710 --> 00:42:59,790
+حوالينه ما انفجرش فبنزل الولد مع كيس الماء إذا نزلت
+
+485
+00:42:59,790 --> 00:43:04,130
+الرأس مع كيس الماء بكل بساطة جش راجية خالص بصابعه
+
+486
+00:43:04,130 --> 00:43:08,690
+جولى فهي هيك بتنمزق وبتنفتح وبيطلع الجنين من
+
+487
+00:43:08,690 --> 00:43:17,370
+وسطها وإلا هيضلوا ينخنق احنا
+
+488
+00:43:17,370 --> 00:43:21,770
+إيش قاعدين بنقول أحيانا التلد بالبولوز هو الأصل
+
+489
+00:43:21,770 --> 00:43:26,950
+ينفجر، الأصل ينفجر قبل الولادة، إذا ما انفجرش إيش
+
+490
+00:43:26,950 --> 00:43:32,690
+قلنا بنساوي؟ بدك تمزقيه منتهى الحين أنت الدكتور،
+
+491
+00:43:32,690 --> 00:43:37,770
+مش احنا قاعدين بنعلمك أن أنت الدكتور اللي بولد؟
+
+492
+00:43:37,770 --> 00:43:42,290
+لا مش شرط، مش شرط، أحيانا بينفجر لحاله، وأحيانا
+
+493
+00:43:42,290 --> 00:43:46,620
+عشان تسرع الولادة بنفجره أحيانا قد نحتاج أحيانا
+
+494
+00:43:46,620 --> 00:43:51,460
+أننا نمزقه فبنفجره فبتلاقي بتنزل المي بتلاقي بندفق
+
+495
+00:43:51,460 --> 00:43:57,100
+الطفل إلى الخارج لكن افترضي أنه ولدة الست مع الكيس
+
+496
+00:43:57,100 --> 00:44:01,480
+الماء إيش دورنا احنا نعمل؟ بكل بساطة كيس رقيق
+
+497
+00:44:01,480 --> 00:44:07,360
+بنمزقه وبتنزل اللي هي الجنين بدون كيس الماء لأنه
+
+498
+00:44:07,360 --> 00:44:13,500
+نضله في الكيس هينخنق فبقى ارفعه وبستقبل الست أو
+
+499
+00:44:13,500 --> 00:44:29,740
+بستقبل الجنين بدون .. فضل هو ممكن
+
+500
+00:44:29,740 --> 00:44:36,580
+يستحمل أيام .. ممكن يستحمل أيام بس مش في الدار مش 
+
+501
+00:44:36,580 --> 00:44:40,180
+في الدار في المستشفى، ليش؟ في المستشفى بنشوف إن
+
+502
+00:44:40,180 --> 00:44:44,620
+الحب السري ملفوف بطريقة صحيحة وفاتح ولا مش فاتح
+
+503
+00:44:44,620 --> 00:44:47,960
+لكن في الدار ممكن يكون مضغوط، الحب السري مسكر عليه
+
+504
+00:44:47,960 --> 00:44:54,380
+وفشت لا نفس بصل ولا تغذية للمخ، ماشي؟ فعشان هيك
+
+505
+00:44:54,380 --> 00:44:59,880
+إحنا لو انفجر في البيت بدنا ننام عكسي ونمنع الحزج
+
+506
+00:44:59,880 --> 00:45:05,100
+ونخليها إيش؟ بطريقة اللي .. نعم؟
+
+507
+00:45:07,000 --> 00:45:11,100
+أنت مش بدك ت .. مش بدك توديها على السيارة، السيارة و
+
+508
+00:45:11,100 --> 00:45:14,600
+هي ممددة على الكرسي، ارفعي رجليها بس وحطي مخدة تحت
+
+509
+00:45:14,600 --> 00:45:18,280
+وسطها بيصير زي هي ولا بدك أكثر من هيك يا ستي
+
+510
+00:45:18,280 --> 00:45:22,660
+ملاشي مخدة، أنت اقعدي وحطي وسطها على رجليك بيصير ..
+
+511
+00:45:22,660 --> 00:45:28,960
+كيف بتصير؟ مستور أنتِ؟ كيف بتصير؟ نفس الفكرة ولا
+
+512
+00:45:28,960 --> 00:45:34,840
+لا؟ فبكل بساطة بيصير يعني أنا من أيام زي ما قلت ال
+
+513
+00:45:34,840 --> 00:45:39,880
+commentingtingالدكتور كان رافض أحطلها غرزة لأن أنا
+
+514
+00:45:39,880 --> 00:45:45,180
+ابتلت بدري فعندها بنت، فروحت أنا على المستشفى طبعا
+
+515
+00:45:45,180 --> 00:45:47,720
+لجيت أنام على سرير عادي، كيف يا أبا قالت والله يا
+
+516
+00:45:47,720 --> 00:45:51,920
+أبا فيه تجول بحستها، فروحت ع رفعتلها سرير، هات إجراء
+
+517
+00:45:51,920 --> 00:45:55,760
+من تحت رفعتهن وعملته بالشكل هات، صاروا ييجوا
+
+518
+00:45:55,760 --> 00:45:59,560
+الدكاترة والممرضين ويطلعوا إيش هذا، أقولهم هذا
+
+519
+00:45:59,560 --> 00:46:04,270
+عشان ما يصيرش حاجة، ولا واحد أكترولا، واحد اعترض خالص
+
+520
+00:46:04,270 --> 00:46:07,570
+وظلت ثلاثة أربعة أيام، أسبوع وهي بالشكل هذا إلى
+
+521
+00:46:07,570 --> 00:46:11,190
+أنهم يعطوها أدوية وأشي اللي تشد الرحم شوية عشان
+
+522
+00:46:11,190 --> 00:46:15,910
+تكمل اللي بتنزل هي كلها عصاة اللي تسمعها ومطبخها
+
+523
+00:46:15,910 --> 00:46:18,910
+و .. هذا .. ايه ده بس .. احنا بنتش .. لا نزلت مي
+
+524
+00:46:18,910 --> 00:46:22,770
+ولا حاجة، إنما هاد جاب المعادة بفترة بدنا نأخر
+
+525
+00:46:22,770 --> 00:46:26,810
+بالظبط، فخليتها بالشكل هاد لأنه بدنا نطول المدة لأ
+
+526
+00:46:26,810 --> 00:46:30,710
+كل ما الجنين قعد يوم زيادة في بطن أمه كل ما كان
+
+527
+00:46:30,710 --> 00:46:36,270
+فرصته في الحياة والصحة
+
+528
+00:46:36,270 --> 00:46:38,810
+أكثر، اليوم بيفرق في حياة الجنين
+
+529
+00:46:41,840 --> 00:46:46,100
+طبعا إذا ظهر أي جزء من جسم الطفل غير الرأس بتكون
+
+530
+00:46:46,100 --> 00:46:53,640
+الولادة مش طبيعية، تكون الولادة متعثرة وعندها طبعا
+
+531
+00:46:53,640 --> 00:46:58,100
+إلى أقرب مستشفى بنبعته وبنمنع للست أن تشد على
+
+532
+00:46:58,100 --> 00:47:05,880
+حالها حتى ما نحاولش ننزل أي جزء آخر الآن
+
+533
+00:47:05,880 --> 00:47:11,080
+نزل الرأس، نزل
+
+534
+00:47:18,070 --> 00:47:20,610
+لما تنزل الرأس يا أخوات المجدات، الستات اللي
+
+535
+00:47:20,610 --> 00:47:25,990
+ولدوا، قعدة مجرد ما تنزل الرأس سهل جدا جدا الباقي
+
+536
+00:47:25,990 --> 00:47:30,390
+ينزل، أكبر جزء قلنا في الجسم اللي هو إيش وأصعب جزء
+
+537
+00:47:30,390 --> 00:47:34,710
+في الجسم اللي هو إيش، الرأس، مجرد ما تنزل الرأس بكل
+
+538
+00:47:34,710 --> 00:47:39,450
+بساطة بقطعة القماشة النظيفة هاد وبالإيقونتي، حطي
+
+539
+00:47:39,450 --> 00:47:43,450
+إصبعك تحت الكتف، تلاقي الكتف ما فيش رقبة للصغير،
+
+540
+00:47:43,450 --> 00:47:47,630
+قداش رقبة الصغير هذا؟fishing بتلاقي دوش رجال
+
+541
+00:47:47,630 --> 00:47:52,790
+تقريبا ليش الفقرات الصغيرة لسه ما نمتش، فبتحطي إصبعك
+
+542
+00:47:52,790 --> 00:47:58,130
+تحت الكتف وبتسحبي بيطلع معاكي بسهولة جدا جدا جدا
+
+543
+00:47:58,130 --> 00:48:02,470
+متناعي الست مجرد ما تنزل الراس بعدها بتحسش كيف نزل
+
+544
+00:48:02,470 --> 00:48:06,290
+الباقي ولا لأ؟ هاي واحدة ولدت وهي كمان واحدة ولدت
+
+545
+00:48:06,290 --> 00:48:09,910
+كيف؟ مجرد ما نزلت الراس حسيت في الباقي؟ حسيت في
+
+546
+00:48:09,910 --> 00:48:10,850
+الباقي؟
+
+547
+00:48:13,420 --> 00:48:18,560
+ربنا يعوض عليك إن شاء الله، فبمجرد ما بتحطي إصبعين
+
+548
+00:48:18,560 --> 00:48:22,920
+تحت القبط وبتسحبي، طبعا بعض الناس بنتق فبيصير خلق
+
+549
+00:48:22,920 --> 00:48:28,620
+كتف، سمعتوا بيجي واحد مخلوق مكتوف كتفه، بدنا طريقة
+
+550
+00:48:28,620 --> 00:48:32,800
+يعني هادية وبسيطة لأن أنتِ بتتعامليش مع خشب ولا مع
+
+551
+00:48:32,800 --> 00:48:39,740
+حديد، بتتعاملي مع جنين لين طري بسيط، بتحملشفبكل
+
+552
+00:48:39,740 --> 00:48:44,520
+بساطة، ما تعكسيهوش، مجرد ما تطلع الراس بتستني الطلجة
+
+553
+00:48:44,520 --> 00:48:49,840
+اللي بعدها مع إصبعين بس تحت القبط بتلاقي الولد مع
+
+554
+00:48:49,840 --> 00:48:57,660
+الدفع والسحب، طلع بكل بساطة ماشي الحال؟ تمام
+
+555
+00:48:59,150 --> 00:49:03,250
+يجب التذكر دائما بأن الطفل مبلل ومشمع وقد ينزلق من
+
+556
+00:49:03,250 --> 00:49:07,790
+بين اليدين بسهولة، عشان هيك بنستقبله بقطعة قماش
+
+557
+00:49:07,790 --> 00:49:13,530
+نظيفة، بنستقبلوش بإيدينا ولا حتى بليجوانتي لحالة
+
+558
+00:49:13,530 --> 00:49:20,310
+لأن الليجوانتي برضه ناعم وبيسوي انزلاق بسهولة
+
+559
+00:49:20,310 --> 00:49:28,110
+الآن ولد البني آدم وطلع من الرحمة
+
+560
+00:49:30,220 --> 00:49:35,340
+الشعر بيقول ولدت كأمك يا ابن آدمة والناس حولك
+
+561
+00:49:35,340 --> 00:49:39,920
+يضحكون سرورة، ايه الحمد لله ولدت ومكيفين مبروك ما
+
+562
+00:49:39,920 --> 00:49:44,440
+جاكي مبروك يا فلان وحلو كله ولا لأ الحمد لله
+
+563
+00:49:44,440 --> 00:49:49,060
+على سلامتها ولدت مبروك، فاعمل ليوم أن تكون إذا بكت
+
+564
+00:49:49,060 --> 00:49:53,020
+الناس حولك ضاحكا مسرورا وكتير الناس بتبكي حوالي
+
+565
+00:49:53,020 --> 00:49:58,070
+.. هو أنا عنعش هو أنا وين؟ محطوط ع الناس طب أنا بدأ
+
+566
+00:49:58,070 --> 00:50:03,410
+أكون ضاحق مصروح كيف بدأ أطلع أشوف الخير اللي أنا
+
+567
+00:50:03,410 --> 00:50:09,070
+مقبل عليه، الجنة، ملائكة الله، الرحمة، التباشير
+
+568
+00:50:09,070 --> 00:50:13,030
+التباشير اللي بيبعتليه يا ربنا سبحانه وتعالى، انعاش
+
+569
+00:50:13,030 --> 00:50:18,110
+الوليد، مجرد ما يلد الولد الأصل أنه يبكي، بكى الولد
+
+570
+00:50:18,110 --> 00:50:26,830
+معناته إيش تنفس، معناته إيش تنفس، معناته في حياة ما
+
+571
+00:50:26,830 --> 00:50:35,310
+بكاش، ممكن يحتاج إلى عملية إنعاش، ممكن يحتاج إلى
+
+572
+00:50:35,310 --> 00:50:42,010
+عملية إنعاش، طبعا مجرد ما يبكي بتلاقي وجهه فتح وحمر
+
+573
+00:50:42,010 --> 00:50:45,850
+وصار تمام، لو قعد فترة بدون ما يبكي بتلاقي زراج
+
+574
+00:50:45,850 --> 00:50:51,190
+بتلاقي زراج، ليش؟ ما فيش أكسجين، ما فيش أكسجين، فالآن أنا
+
+575
+00:50:51,190 --> 00:50:58,300
+دوري إيش؟ دوري أنه إذا ما بكاش أخليه يتنفس، مش شرط
+
+576
+00:50:58,300 --> 00:51:02,280
+بكلمهم يتنفس، كيف بخليه يتنفس؟ قطعة لقماش في يدي
+
+577
+00:51:02,280 --> 00:51:10,360
+بمسك رجلين اتنين وإصبعين، إيش هذه الحركة؟ ممكن
+
+578
+00:51:10,360 --> 00:51:14,620
+تعمل بتنشط الجهاز التنفسي، الحاجة التانية إذا كانوا
+
+579
+00:51:14,620 --> 00:51:19,520
+شارب من الماء أثناء الولادة ومسكر نفسه بتنزل
+
+580
+00:51:20,580 --> 00:51:24,620
+متشردج بالظبط، بتلاقي هي نزلت، فمجرد ما تنزل إيش
+
+581
+00:51:24,620 --> 00:51:30,680
+بيصير؟ تنفتح مسلك التنفس أو مجاري التنفس فبتلاقي
+
+582
+00:51:30,680 --> 00:51:37,040
+إيش؟ بتنفس بعدها، طبعا بعض الأولاد مجرد بس تدفي
+
+583
+00:51:37,040 --> 00:51:41,340
+إذا كان الدنيا برد وسجعة أو إيش زي هي مجرد إنك
+
+584
+00:51:41,340 --> 00:51:46,380
+تدفي بتلاقي بيستعيد قوته وعافيته وبيتنفس وبيصير
+
+585
+00:51:46,380 --> 00:51:47,560
+وضعه كويس
+
+586
+00:51:54,550 --> 00:51:59,910
+لأ، هاي حالة الحبل السري لف عرّجت، ماشي، سأولكوا
+
+587
+00:51:59,910 --> 00:52:04,350
+الحبل السري؟ حطوا إصبعهم وجمه؟ لأ، هو نزلوا على
+
+588
+00:52:04,350 --> 00:52:08,690
+ردد يعني نزل كله كان على ردد وما كان يتنقل، فتقريبا
+
+589
+00:52:08,690 --> 00:52:15,950
+عشر دفاع يكون الاستعاف وهكتر لأنه بيجيب عشرة
+
+590
+00:52:15,950 --> 00:52:19,310
+دفاع، لحمد الله يعني يمكن مش عشرة يمكن أقل شوية بس
+
+591
+00:52:19,310 --> 00:52:20,250
+أنتِ شفتيه عشرة
+
+592
+00:52:28,590 --> 00:52:35,510
+ملفوف لف بس مش مسكر يعني لسه بياخد أكسجين منك
+
+593
+00:52:35,510 --> 00:52:41,370
+بيصير
+
+594
+00:52:41,370 --> 00:52:44,770
+ضرب في المخ بيصير مشكلة في المخ
+
+595
+00:52:49,310 --> 00:52:54,450
+احنا كيف بنعمله إن عاش الطفل؟ بكل بساطة إذا ما
+
+596
+00:52:54,450 --> 00:52:58,250
+اتنفسش بنخليه إن شاء الله يتنفس، كيف بنتنفس؟ بنمسكه
+
+597
+00:52:58,250 --> 00:53:03,910
+من رجليه بقطعة القماش النظيفة الجافة وبضرب بسيط
+
+598
+00:53:08,190 --> 00:53:11,870
+ما هي انفعلت، هي دي ريبالك انفعلت، أنتو عارفين إن
+
+599
+00:53:11,870 --> 00:53:15,710
+الواحد لما يبقى عيش تغل أحيانا قد يصاب بنفعلات
+
+600
+00:53:15,710 --> 00:53:20,210
+شديدة و .. هي بتخاف أكثر منك، دي ريبالك يومك حتى
+
+601
+00:53:20,210 --> 00:53:24,630
+كانت إن معايا الصواريخ، رياء هي ما سيكتوف، تضرب تضرب
+
+602
+00:53:24,630 --> 00:53:30,500
+يعني ضرب حاجة صارت صارت زلزلة، الأصل أنه شوية خبط
+
+603
+00:53:30,500 --> 00:53:36,280
+هيك مع تنظيف للإفرازات اللي في التم بتلاقي الولد
+
+604
+00:53:36,280 --> 00:53:41,980
+ممكن جدا بعديها صار يتنفس، مظبوط؟ ما صارش يتنفس
+
+605
+00:53:41,980 --> 00:53:45,660
+بضطر أعمله تنفس صناعي، كيف التنفس الصناعي؟ بنمسح
+
+606
+00:53:45,660 --> 00:53:51,320
+تمه وبنحط نفخ، دبّت البوج في تمه كذا مرة، ممكن يصير
+
+607
+00:53:51,320 --> 00:53:59,690
+بعديها اللي هو يتنفس، أو بنيجي ندلكله صدره، ندلكله
+
+608
+00:53:59,690 --> 00:54:07,030
+ظهره وبنصفعه على بطن قدمه، كل هذه شغلات بتعمل وزي
+
+609
+00:54:07,030 --> 00:54:11,470
+ما قلنا لو بعض الأطفال بس مجرد تدفيه ممكن أنه إيش
+
+610
+00:54:11,470 --> 00:54:19,750
+يبدأ يتنفس ويصير وضعه كويس، مجرد قلنا، تلادي الحالة
+
+611
+00:54:20,300 --> 00:54:23,700
+عندما تكون الأم قوية تكون قادرة على المساعدة
+
+612
+00:54:23,700 --> 00:54:27,540
+ويمكنها الإرضاع بطفلها وهو على بطنها، فنحط الطفل
+
+613
+00:54:27,540 --> 00:54:31,520
+على بطنها، ومجرد ما تطلع من الكشف بنقولها ارضعيه
+
+614
+00:54:32,450 --> 00:54:37,030
+مجرد ما تطلع من الكشك بنقولها ارضعيه، ليش ارضعيه؟
+
+615
+00:54:37,030 --> 00:54:42,150
+تنشيط لخلايا الشذي، خلايا الحليب، أطاقه كل هيت
+
+616
+00:54:42,150 --> 00:54:47,110
+المتطعمات اللي في الأم، وبعدين بتنشط اللي هو
+
+617
+00:54:47,110 --> 00:54:51,630
+المضادات الحيوية اللي في جسم الإنسان، كلها بتلاقي
+
+618
+00:54:51,630 --> 00:54:57,710
+موجود منها في حليب اللي بقى، هذا بالنسبة لكيف بدنا
+
+619
+00:54:57,710 --> 00:55:03,030
+نولد الموضوع، في أي سؤال؟ مين هيك يعني عندها أي
+
+620
+00:55:03,030 --> 00:55:07,170
+سؤال أو استفسار؟ مين شافت أشي غير اللي قلناها؟
+
+621
+00:55:07,170 --> 00:55:11,770
+قولي اطلاقا
+
+622
+00:55:11,770 --> 00:55:16,810
+لأ، ضغطوا على بطنك لما قلتي؟ ضغطوا؟ على أمهم، لأ،
+
+623
+00:55:16,810 --> 00:55:26,430
+مش لازم، أنا في حالة يمكن شفته الجماعة اللي اجوا
+
+624
+00:55:26,430 --> 00:55:35,700
+شفتهم، واحد منهم عنده ولد وربنا بعد يمكن عشر سنين
+
+625
+00:55:35,700 --> 00:55:41,480
+بعتله كمان ولد يعني ولد عزيز، فإيش ساووا معاه في 
+
+626
+00:55:41,480 --> 00:55:47,660
+الشفاة؟ في الشفاة كاينين أجي مصيبة مع هالواحدة
+
+627
+00:55:47,660 --> 00:55:53,920
+تشتغل غلط إيش صار؟ والأم بدها تولد دبّت حالها فوق
+
+628
+00:55:53,920 --> 00:55:59,300
+بطلها جاعدت فوق بطلها فطلع الولد دفك مخبطة رأسه في
+
+629
+00:55:59,300 --> 00:56:04,710
+الأرض، والسنينها الحين بيعانى، وبودّى على الداخل و
+
+630
+00:56:04,710 --> 00:56:09,630
+بودّى على الدنيا، والولد مش طبيعى من إيش؟ من الضغط
+
+631
+00:56:09,630 --> 00:56:16,150
+على البطن اخت
+
+632
+00:56:16,150 --> 00:56:20,770
+اليوم في علاج، بنعطى في المحلول، بزيد الطلج، وبزيد
+
+633
+00:56:20,770 --> 00:56:30,790
+الدفعة فشيوسع بنعمل اللي هو .. بنعمل بنعمل اللي هي
+
+634
+00:56:30,790 --> 00:56:36,230
+المزق أو الجرح اللي بنعمله لكل بكريه اليوم كل
+
+635
+00:56:36,230 --> 00:56:41,070
+بكريه اليوم بنعمللها جرح جانب بحيث إنه تتوسع
+
+636
+00:56:41,070 --> 00:56:46,090
+المنطقة ويطلع الجنين المهم
+
+637
+00:56:46,090 --> 00:56:53,020
+يعني .. يعني ممكن هذا اضطرار بحضر ضغط بحضر مش زي ال
+
+638
+00:56:53,020 --> 00:56:57,000
+.. الست اللي بتقعد فوق بطن الملعب عنده ولد وعنده
+
+639
+00:56:57,000 --> 00:56:59,740
+الولد الثاني ربنا بعته إياه، ومن يوم مولده لليوم، و
+
+640
+00:56:59,740 --> 00:57:04,380
+هو يجري اللي هو ده الآن بيجي سنتين وشوية من إيش؟
+
+641
+00:57:04,380 --> 00:57:08,180
+من سيدتها اللي شاطرة وأجد تقعد فوق بطن اللي هو
+
+642
+00:57:08,180 --> 00:57:17,440
+طبعا ..إذا كان الحبل ملتف حوال عنق الطفل فهذا طبعا
+
+643
+00:57:17,440 --> 00:57:22,100
+بيعمله مشكلة إذا ما عرفناش ننزل الطفل ونحرر العنق
+
+644
+00:57:22,100 --> 00:57:27,020
+بدنا نضطر نقطع الحبل السري، لكن إذا ولد بدون مشكلة
+
+645
+00:57:27,020 --> 00:57:31,020
+وفي طول بدون ما يخنقه، أنت بتقول يخنقه، كان الأصل
+
+646
+00:57:31,020 --> 00:57:35,820
+أنه يقطعوا يلموا إيديهم كده ويقصوا بين اثنين، و
+
+647
+00:57:35,820 --> 00:57:41,870
+الحمد لله اللي ربنا سلم، يعني هذا من نصيبه ولا يقطع
+
+648
+00:57:41,870 --> 00:57:45,070
+الحبل السري إلا عندما تتوقف كل نبضات فيه، قولنا
+
+649
+00:57:45,070 --> 00:57:49,350
+بقول حوالي دقيقتين أو تلاتة وهو فيه نبضات، ليش؟
+
+650
+00:57:49,350 --> 00:57:53,150
+خلي الولد يستفيد من ال oxygen ومن الدم كمان،
+
+651
+00:57:53,150 --> 00:57:56,330
+قاطعتيه خلاص، بطل ياخد ولا نقطة دم، لكن إن ظلو
+
+652
+00:57:56,330 --> 00:58:00,870
+يمشي، بظل يعطيه اللي هي بعد حوالي خمس دقايق يقطع
+
+653
+00:58:00,870 --> 00:58:07,090
+الحبل السري على بعد عشرة دقايق من البطلعشرة .. عشرة
+
+654
+00:58:07,090 --> 00:58:11,410
+ثانية من البطن تيجي فوق الصرة فوق مكان الصرة
+
+655
+00:58:11,410 --> 00:58:16,730
+مربوطة في البطن بحوالي عشرة ثانية بنحط مربط فش
+
+656
+00:58:16,730 --> 00:58:22,530
+مربط بنحط رباط عريض بحطش خيط رفيع ليش بحطش خيط
+
+657
+00:58:22,530 --> 00:58:27,810
+رفيع لأن خيط رفيع انشدته بيمزع الحبل السري فبدي
+
+658
+00:58:27,810 --> 00:58:34,390
+أحط خيط عريض أو ملقط أو مربط ماشي وبده أحط مربط
+
+659
+00:58:34,390 --> 00:58:41,090
+يحمي الجنين ومربط يحمي الأم فبين التنين بقى قص
+
+660
+00:58:41,090 --> 00:58:45,750
+بأداة معقمة بين التنين على بعد عشرة سنتي بقص
+
+661
+00:58:45,750 --> 00:58:51,880
+بأداة معقمة لأنه إذا ما ارتبطش إرباطين، إرباط هيكون
+
+662
+00:58:51,880 --> 00:58:55,440
+دم الولد مسكر عليه وإرباط هيكون دم الأم مسكر
+
+663
+00:58:55,440 --> 00:58:59,020
+عليها إذا ما ارتبطوش اثنين، اللي تلاقي مش مربوط،
+
+664
+00:58:59,020 --> 00:59:03,460
+هيصير ينزف، يعني الست هيصير تنزف أو الطفل هيصير
+
+665
+00:59:03,460 --> 00:59:09,960
+ينزف، طبعا هو اللي بدا يقص ويحط مرابط، جثيته بعدين
+
+666
+00:59:09,960 --> 00:59:16,460
+بتنتلي دم، جثيته بتنتلي دم، فبتحط المرابط أول أو
+
+667
+00:59:16,460 --> 00:59:22,720
+الإرباط ثم يقص بأداة معقمة فإيش عندك أداة معقمة
+
+668
+00:59:22,720 --> 00:59:29,520
+ما تجصيش بتاخد ال machine أو بتاخد الولد وبتوديه
+
+669
+00:59:29,520 --> 00:59:33,640
+على مركز يجصه بأداة معقمة أو بتعقم أداة وبتجصي
+
+670
+00:59:33,640 --> 00:59:39,540
+واضح؟ ليش؟ لأن الأداة المش معقمة هاد هتنجل الولد
+
+671
+00:59:39,540 --> 00:59:45,620
+بلاوي وتعمله مشكلة، احنا في غنانة فليش أغلب حالي وأغلب
+
+672
+00:59:45,620 --> 00:59:47,000
+أغلب الجنين مسكين
+
+673
+00:59:51,340 --> 00:59:57,380
+الآن الحمد لله ولدت وربنا عوض عليها واتنفس
+
+674
+00:59:57,380 --> 01:00:07,500
+الولد وغيرناه ولبسناه ونظفنا للأم ولبسناها بعد
+
+675
+01:00:07,500 --> 01:00:12,840
+ما تنزل المشيمة واكتشف بدنا نلبس الأم وننظفها بعد
+
+676
+01:00:12,840 --> 01:00:17,200
+ما تنزل المشيمة قبل ما تنزل المشيمة الست لسه في
+
+677
+01:00:17,200 --> 01:00:23,300
+حالة ولادة ما خلصتش، كيف بتنزل المشيمة؟ المشيمة احنا
+
+678
+01:00:23,300 --> 01:00:27,740
+ربطناها من تلال الأم، ماشي؟ بتنزل المشيمة الستات
+
+679
+01:00:27,740 --> 01:00:31,460
+اللي ولدوا كيف نزلت؟
+
+680
+01:00:31,460 --> 01:00:37,200
+بيجي طلقة أو طلقتين أو تلاتة، ماشي؟ بتطلع المشيمة
+
+681
+01:00:37,200 --> 01:00:42,540
+منهم كأنها ولاد جديدة كان هولاد جديدة بالظبط طبعا
+
+682
+01:00:42,540 --> 01:00:47,160
+خطأ جدا أن تشد الحبل السري عشان تطلع المشيمة وكمان
+
+683
+01:00:47,160 --> 01:00:50,300
+خطأ أنك تضغط على بطن الأم عشان تطلع المشيمة سيبيها
+
+684
+01:00:50,300 --> 01:00:57,210
+لحالها المشيمة إيش بتنزل وبتصير يعني عبارة عن كأنه
+
+685
+01:00:57,210 --> 01:01:01,990
+ولادة جديدة طبعا بألم أخف شوية من ولادة اللي هو
+
+686
+01:01:01,990 --> 01:01:08,930
+الجنين يبدأ الطلق مجددا بعد ولادة الطفل تحضير لطرح
+
+687
+01:01:08,930 --> 01:01:12,990
+المشيمة بعد انفصاله عن جدار الرحم يجب عدم شد الحبل
+
+688
+01:01:12,990 --> 01:01:16,770
+السري أو حتى الضغط على البطن بغية الإسراع في هذه
+
+689
+01:01:16,770 --> 01:01:21,550
+العملية لأن ذلك قد يسبب أذى كثير للأم، ما نزيف
+
+690
+01:01:21,550 --> 01:01:26,610
+قاتل أو انقلاب للرحم، تسمع لو الرحم مجلوب، الست
+
+691
+01:01:26,610 --> 01:01:33,150
+هذه للرحم مجلوب أو معكوس أو أعوج فبتلاقي مع شد
+
+692
+01:01:33,150 --> 01:01:37,110
+الخلاصة ممكن إيش يصير ينتنى لأن الخلاصة على جنب
+
+693
+01:01:37,110 --> 01:01:42,500
+فبتشديها بتنعدل أو بتنسلخ سلخ تعمل نزيف، فسيبيها
+
+694
+01:01:42,500 --> 01:01:48,800
+الست لحالها بتلد .. بتنزل المشيمة أو الخلاصة
+
+695
+01:01:48,800 --> 01:01:56,560
+بطريقتين متأخرات علشان إيش تنزل منها الخلاصة طبعا
+
+696
+01:01:56,560 --> 01:02:01,500
+إنت دورك كمسعف لما تلد الست اتفجد الخلاصة إيش يعني
+
+697
+01:02:01,500 --> 01:02:06,660
+اتفجد الخلاصة؟ يعني أطلع هل الخلاصة كاملة أو رايحة
+
+698
+01:02:06,660 --> 01:02:10,220
+منها شجفة، إذا رايحة منها شجفة ماذا يعني؟
+
+699
+01:02:12,980 --> 01:02:18,720
+ظلت معلقة في الرحم، يعني
+
+700
+01:02:18,720 --> 01:02:24,480
+ظلت الست مفتوحة على فيه الدم ظلت الست مفتوحة عن فيه
+
+701
+01:02:24,480 --> 01:02:28,140
+الدم بتنزل من دم الطبيعي ما فيش حاجة اسمها دم
+
+702
+01:02:28,140 --> 01:02:33,380
+مشنافع أو دم فاسد أو دم بعد الولادة لا الأصل إنه
+
+703
+01:02:33,380 --> 01:02:38,400
+ما يظلش ولا ملي من المشيمة معلق في الرحم، إن ظل جزء
+
+704
+01:02:38,400 --> 01:02:43,180
+من المشيمة معلق في الرحم يعني هذا إن الست أو الأم
+
+705
+01:02:43,180 --> 01:02:49,220
+ستبقى تنزف حتى ينزلها، وكتير بتسمع إن بعد ما ولدت
+
+706
+01:02:49,220 --> 01:02:53,930
+وجدت على الكشف ورجعوها ثاني على مجموعة عمليات، تنظيفات
+
+707
+01:02:53,930 --> 01:02:59,170
+عملية إيش؟ شو يعني عملية تنظيفات؟ يعني عملوا
+
+708
+01:02:59,170 --> 01:03:06,490
+بيسموها كحت وتنظيف، كحت وتنظيف، كحت بيكون في حاجة
+
+709
+01:03:06,490 --> 01:03:16,250
+زي كحاتة يعني هيك شكلها بالظبط
+
+710
+01:03:16,250 --> 01:03:24,840
+زي هيك هذه حفتها يعني شوية حادة بتدخلوها وبتكحط زي
+
+711
+01:03:24,840 --> 01:03:28,100
+اللي بنجحط فيها جشر البطاطا وجشر الكساية وجشر
+
+712
+01:03:28,100 --> 01:03:35,020
+هذا بس على حجم أصغر بتكحط الجزء اللي متبقي من اللي
+
+713
+01:03:35,020 --> 01:03:38,800
+هو المشيمة، ومجرد ما تكحطي بفضل الله بيجف اللي هو
+
+714
+01:03:38,800 --> 01:03:42,900
+إيش النظيف وبتبطل الست تنزف
+
+715
+01:03:49,050 --> 01:03:56,510
+ممكن المشيمة تطلع على الجلد يعني؟ أنا حضرت مرة قبل
+
+716
+01:03:56,510 --> 01:04:00,290
+ولادة إن كانت في الحرب، روحت السترة بتطلها، وطل من
+
+717
+01:04:00,290 --> 01:04:03,550
+فجر عشان ما يطلع على الجلد اللي كانت بتفهم كده
+
+718
+01:04:03,550 --> 01:04:08,770
+يطلع الجلد، مش عارفة ليش جلدت هيك، نزلت المشيمة ولا
+
+719
+01:04:08,770 --> 01:04:13,490
+ما نزلتش؟ طولت طولت لأنها ربطتها
+
+720
+01:04:17,610 --> 01:04:21,910
+الصدق إن المرض بتهاش كان يعني نزلت بسهولة أحسن،
+
+721
+01:04:21,910 --> 01:04:26,670
+بوجهة أسرع، أصلا تعالى نتفرج، في عندك حاجة اسمها
+
+722
+01:04:26,670 --> 01:04:31,290
+الحجاب الحاجز وحاجة اسمها جدار البطن وحاجة اسمها
+
+723
+01:04:31,290 --> 01:04:36,650
+القفص الصدري، كل هذا حاجز البطن عن الشسم، عن
+
+724
+01:04:36,650 --> 01:04:42,530
+القلب، كيف بدها تصل الجانب؟ يعني ارجع للتشريح أو
+
+725
+01:04:42,530 --> 01:04:47,510
+ارجع لصورة البناء ده ما فيش إمكانية مطلقا إنه تدخل
+
+726
+01:04:47,510 --> 01:04:52,230
+الجنين بالظبط بقعد بقولك زمان كانوا كيف .. مين اللي
+
+727
+01:04:52,230 --> 01:04:56,990
+والدها، أنا بعرف الست بقعد تصدقه ما تعرف تكتب اسمها
+
+728
+01:04:56,990 --> 01:05:01,930
+ودمارها عجوز اختيارة، وحتى بتشوفش كويس من كتر كبر
+
+729
+01:05:01,930 --> 01:05:05,590
+السن وكانت مشهورة جدا في التوليد، مش ملاعقة في
+
+730
+01:05:05,590 --> 01:05:09,610
+المنطقة لا مش مشهورة .. لا بحكيلك يعني .. نعم
+
+731
+01:05:12,240 --> 01:05:17,680
+بالظبط بالظبط فبمعنى إنه يعني المشيمة هذه ما فيش
+
+732
+01:05:17,680 --> 01:05:23,180
+إمكانية أنها تطلع على القلب بعد الولادة يا أخواتي
+
+733
+01:05:23,180 --> 01:05:28,360
+المجدات طبعا يعني حسب خبرتي في النسوان في ستات
+
+734
+01:05:28,360 --> 01:05:34,160
+بتموت من الجوع مجرد ما تخلص ميلاد بتهفت بدها أي
+
+735
+01:05:34,160 --> 01:05:42,040
+حاجة تكلها بدها أي حاجة تحط في بطنها ليش الجنين
+
+736
+01:05:42,040 --> 01:05:46,800
+هالجد هو المي والخلاصة وبل البطن هالحين فضل
+
+737
+01:05:46,800 --> 01:05:54,100
+البطن فصار حاسق بخوائق بدأ تملي فبكل بساطة ممكن
+
+738
+01:05:54,100 --> 01:05:59,440
+نديها مشروب ونديها أكل وأفضل حاجة تديها التمر
+
+739
+01:05:59,440 --> 01:06:07,940
+وهزي إليك بجلة نخلة تساقط عليك رطبا جنيه فاكلي و
+
+740
+01:06:07,940 --> 01:06:14,000
+اشربي وقري عينك، الطفل بتحنج من التمر والأم لو
+
+741
+01:06:14,000 --> 01:06:18,700
+أكلت تمر بيساعد على عودة الجنين اللي رحم إلى وضعه
+
+742
+01:06:18,700 --> 01:06:24,660
+الطبيعي وشده وتخفيف نزول الدم بعد الولادة ماخذ
+
+743
+01:06:24,660 --> 01:06:28,920
+بالك وتنشيط نزول الحليب فالتمر يعني مش أنا اللي
+
+744
+01:06:28,920 --> 01:06:35,840
+بقول الله جال يتساقط عليك رطبا وجال عنه إيش فاكلي
+
+745
+01:06:35,840 --> 01:06:43,510
+فيه مي وفيه ضعم فيه غذاء اللي بيأكل إنطمر قبل
+
+746
+01:06:43,510 --> 01:06:48,530
+الطلج بفترة ممكن تصير ولادة سريعة الرطب أو التمر
+
+747
+01:06:48,530 --> 01:06:52,770
+و
+
+748
+01:06:52,770 --> 01:06:55,970
+ممكن
+
+749
+01:06:55,970 --> 01:07:00,850
+الجرفة هذه .. الجرفة هذه بيستخدموها لتنشيط المبايض
+
+750
+01:07:00,850 --> 01:07:06,570
+ما بقولك لتنشيط المبايض في حالة .. قبل الحمل أما 
+
+751
+01:07:06,570 --> 01:07:08,430
+بعد الولادة مش عارف يعني
+
+752
+01:07:16,560 --> 01:07:24,320
+ممكن مش لأ، ممكن، ممكن، إذا إيه جنة؟ جنة بننظف
+
+753
+01:07:24,320 --> 01:07:28,440
+الست و بنلبسها و بن .. زي ما قلنا لك و في ستات
+
+754
+01:07:28,440 --> 01:07:33,900
+بتصير هفتانة، بدها أي حاجة تحط في بطنها فبنطعمها و
+
+755
+01:07:33,900 --> 01:07:38,940
+بنسجيها إذا بدها و في ستات بتنسد نفسها خالص ما فيش 
+
+756
+01:07:38,940 --> 01:07:43,420
+عندها استخدال لأي نوع من الأكل أو الشرب يمكن اسمته
+
+757
+01:07:43,420 --> 01:07:44,200
+بحاجات زي هيك
+
+758
+01:07:47,160 --> 01:07:50,020
+المهم اه في ستات خلاص ما عندهاش استعداد تاكل والدها
+
+759
+01:07:50,020 --> 01:07:55,440
+تشرب ومش قادرة فبننظفها بنسجيها شيء دافي، شاي،
+
+760
+01:07:55,440 --> 01:08:01,320
+جهوى، شراب، بنديها ساندوش، الطفل بندير بن عليه
+
+761
+01:08:01,320 --> 01:08:05,400
+بإنه نتأكد أنه تنفس طبيعي، الطفل مجرد ميلد
+
+762
+01:08:05,400 --> 01:08:09,970
+ما تناموش على ظهره بعد ما ترضيهم مجرد ما ترد عينيه
+
+763
+01:08:09,970 --> 01:08:13,690
+يميه على جنبه لإنه ممكن ينتج و صغير هذا بنخنج و
+
+764
+01:08:13,690 --> 01:08:18,780
+أنت مش داري فبنطبطب على ظهره وبنعمله تتريات تسمع
+
+765
+01:08:18,780 --> 01:08:23,560
+تطرع الطفل بعد الرضعة .. بعد كل رضعة لمدة ما يصير
+
+766
+01:08:23,560 --> 01:08:29,440
+الولد يتحكم بحاله بنضلنا نطرعه و بنخليه على جنبه و
+
+767
+01:08:29,440 --> 01:08:34,160
+زي ما قلنا بنحاولش نجيم المادة الشمعية اللي موجودة
+
+768
+01:08:34,160 --> 01:08:38,960
+على جسمه لمدة أقل حاجة 24 ساعة و لو كان 48 ساعة
+
+769
+01:08:38,960 --> 01:08:44,000
+بيكون أفضل لأن جسمه بتغذى منها و بيستفيد منها طبعا
+
+770
+01:08:44,000 --> 01:08:49,370
+ممكن يصير خطر نظيف لذلك يجب البحث عن عوام طبي دون
+
+771
+01:08:49,370 --> 01:08:53,850
+ما تأخير زي
+
+772
+01:08:53,850 --> 01:08:58,270
+ما قالت الأخت قعدت مشي متقامة ساعة مش راضي تنزل مش
+
+773
+01:08:58,270 --> 01:09:05,090
+راضي تنزل ساعة معناته فيه مشكلة ماشي؟ في خلال ربع
+
+774
+01:09:05,090 --> 01:09:09,950
+ساعة إلى تلت ساعة قد ايش قعدت لما نزلت؟ من خلال ربع
+
+775
+01:09:09,950 --> 01:09:14,960
+ساعة إلى تلت ساعة انطرت بتحمل نصأحنا قلنا إذا
+
+776
+01:09:14,960 --> 01:09:19,020
+اضطرينا للتدليق البسيط بمشي بس مش دب أو حاجة زي
+
+777
+01:09:19,020 --> 01:09:23,360
+هيك أما هي في الوضع الطبيعي بتنزل في خلال ربع ساعة
+
+778
+01:09:23,360 --> 01:09:31,960
+إن طارت نص ساعة بتكون ال set نزلت ليه؟ إيش الأدوات
+
+779
+01:09:31,960 --> 01:09:34,820
+اللي بنحتاجها في الولادة؟ إيش قلنا يا خبايا بنحتاج
+
+780
+01:09:34,820 --> 01:09:39,860
+في الولادة؟ جماش
+
+781
+01:09:39,860 --> 01:09:47,810
+معقم أو نضيف شرشف عشان الست تمدل عليه ربط للحبل
+
+782
+01:09:47,810 --> 01:09:54,330
+السري شيء عشان اجسس السرة حاد ومعقم طبعا جسيت
+
+783
+01:09:54,330 --> 01:09:59,530
+السرة يا صبايا صار جرح الجرح بده ايه غيار فبده
+
+784
+01:09:59,530 --> 01:10:04,190
+شاش معقم و بعد الشاش نحط لفاف كثير من النسوان
+
+785
+01:10:04,190 --> 01:10:09,370
+بيقولك حط ملح و في ناس بيقولك حط غيار أنا بنصح إن
+
+786
+01:10:09,370 --> 01:10:15,290
+السرة تتغطى و بس ما تحطيش ولا حاجة، غطيها و بس،
+
+787
+01:10:15,290 --> 01:10:18,790
+يعني ممكن تمسحيها بسميرته أول يوم أول مرة، إذا
+
+788
+01:10:18,790 --> 01:10:27,330
+ضليتك تغير عليها، هتطول لما تنشف أكرم
+
+789
+01:10:27,330 --> 01:10:31,750
+يوم قعدت لما نزلت؟ أسبوع، لكن لو ما عصرتيش بطنها،
+
+790
+01:10:31,750 --> 01:10:37,320
+كان أربع أيام خمس نزلت صدقي إنه ما تحطيش ولا حاجة
+
+791
+01:10:37,320 --> 01:10:41,040
+على السرة أول يوم بس بنغطيها و بنغيرها اللي هو
+
+792
+01:10:41,040 --> 01:10:46,100
+بنغطيها و أسيبيها أربع خمس أيام بتكون سمرت و نزلت
+
+793
+01:10:46,100 --> 01:10:51,740
+لحالة إذا ما فيش لزوم للغيار ما تغيرش يعني ممكن الولد
+
+794
+01:10:51,740 --> 01:10:57,420
+يوسخ و يوصلها بغيرها غير هيك لا و بغيار ناشر بغيار
+
+795
+01:10:57,420 --> 01:10:58,040
+ناشر
+
+796
+01:11:01,340 --> 01:11:06,500
+في عجاز بتحط ملح الملح ممكن ينشفها و يوجعها الملح
+
+797
+01:11:06,500 --> 01:11:11,260
+بس ليش هتحط ملح يعني ما فيش دعم هي سيبيها لأ بيحطوا
+
+798
+01:11:11,260 --> 01:11:15,360
+ملح لحاله صدقي
+
+799
+01:11:15,360 --> 01:11:20,600
+أنه بس خطيها و خلاص بتعفنش
+
+800
+01:11:20,600 --> 01:11:25,760
+احنا قلنا أول يوم بتنظف و بتغير و بتحط غيار إذا
+
+801
+01:11:25,760 --> 01:11:31,440
+اضطريت تغير إيه يعني الولد فان و وصلها أتبرز و وصلها
+
+802
+01:11:31,440 --> 01:11:37,400
+بده غيار مش ما تحطيش ولا إشي بمعنى تتعفن ما تتعفن لأ
+
+803
+01:11:37,400 --> 01:11:43,000
+أما هي سبيها إذا ظلت نظيفة أسبوع زمان انطارت بتنزل
+
+804
+01:11:43,000 --> 01:11:50,500
+ماشي طبعا يعني بدنا أداة للجاس وإذا كان المنطق
+
+805
+01:11:50,500 --> 01:11:55,220
+بدها إدارة ويعني وحاجة نحط فيها المشيمة
+
+806
+01:12:00,360 --> 01:12:12,440
+الآن مين عندها أي سؤال؟ قول الوضع الطبيعي صح؟
+
+807
+01:12:12,440 --> 01:12:15,540
+الآن
+
+808
+01:12:15,540 --> 01:12:22,740
+بدنا إيش نقول ليش الستات بيعملوا قيصرية مش في ستات
+
+809
+01:12:22,740 --> 01:12:27,760
+بتعمل قيصرية يعني
+
+810
+01:12:27,760 --> 01:12:28,420
+إيش؟
+
+811
+01:12:34,510 --> 01:12:41,590
+أول حاجة اللي زرعت ليش اللي زرعت .. ليش اللي زرعت
+
+812
+01:12:41,590 --> 01:12:45,890
+.. ليش اللي زرعت ابن عمالها قيصرية؟ هي وقت إيش
+
+813
+01:12:45,890 --> 01:12:53,290
+زرعت هذه؟ بعد خمسة عشر سنة من الجوازة مش هيك؟
+
+814
+01:12:53,290 --> 01:13:00,340
+عشرة ما فيش واحدة بتزرع بعد أسبوع ما تتجوز ولا بعد
+
+815
+01:13:00,340 --> 01:13:08,700
+سنة لأ مش ممكن بعد سنة بتبدأ تتحكم أما تزرع بدأ
+
+816
+01:13:08,700 --> 01:13:15,200
+أقل حاجة تلت سنين إلا إذا .. إلا إذا معروف إنه في
+
+817
+01:13:15,200 --> 01:13:20,500
+مشكلة معروف في مشكلة إذا معروف إنه في مشكلة في قصة
+
+818
+01:13:20,500 --> 01:13:27,560
+عائلة يعني اللي والزلمة مثلا أخواته ما بيخلفوش متجوز
+
+819
+01:13:27,560 --> 01:13:34,140
+قبل هيك واحدة مثلا ومخلفتش في حاجات معينة ماشي
+
+820
+01:13:34,140 --> 01:13:40,220
+فالآن الولد العزيز بيسموه الولد العزيز الولد
+
+821
+01:13:40,220 --> 01:13:44,540
+العزيز ما بدناش نخليه يخش في مراحل الولادة تنزل الراس
+
+822
+01:13:44,540 --> 01:13:50,420
+تجي على الراس ينخنج أثناء الولادة ينزف في ..
+
+823
+01:13:50,420 --> 01:13:56,410
+فبيعملوا لها قيصرية الست اللي عندها ولد إلها الحين
+
+824
+01:13:56,410 --> 01:14:01,070
+يعني .. يعني بندور عليه زي ما بيقولوا دوعة ماشي؟
+
+825
+01:14:01,070 --> 01:14:05,250
+هذا بنعمله قيصرية النوع الثاني من الستات اللي
+
+826
+01:14:05,250 --> 01:14:11,290
+بتحتاج قيصرية مين اللي عندها تسمم حمل اللي هو ضغط
+
+827
+01:14:11,290 --> 01:14:16,990
+و زلال في ال ball و تنفخ في جسمها تلاقي جسمها
+
+828
+01:14:16,990 --> 01:14:22,870
+إيديها منفخات وجهها منفخ جريها منفخات كل شكلها جد
+
+829
+01:14:22,870 --> 01:14:27,890
+ما هي مرتين صارت زي البلون جد ما هي مرتين هذه الست
+
+830
+01:14:27,890 --> 01:14:33,550
+طبعا ممكن جدا لو سبناها تطلق و تتعرض للطلق ممكن
+
+831
+01:14:33,550 --> 01:14:38,470
+جدا يزيد الضغط و تخش في مشاكل و يغم عليها و حالات
+
+832
+01:14:38,470 --> 01:14:43,730
+تسمم الحمل بيسموها هذه حالات تسمم الحمل هذه طبعا
+
+833
+01:14:43,730 --> 01:14:48,550
+أسهل إليها وأيصر إليها و للجنين إنه نعمل إليها
+
+834
+01:14:48,550 --> 01:14:55,680
+إيش قيصرية قيصرية أصعبها الله ليهم صارت يعني مدير ولا
+
+835
+01:14:55,680 --> 01:14:59,500
+مش ملاحظين؟ اللي ليهم صارت مدير من الحاجات اللي
+
+836
+01:14:59,500 --> 01:15:06,180
+بنعملها القيصرية إيش كمان الطفل ييجي جاعد جعود
+
+837
+01:15:06,180 --> 01:15:15,640
+بالعرض ممكن نعدله تلاقي جاعد راسه فوقو إجراء و ..
+
+838
+01:15:15,640 --> 01:15:21,860
+و نصه تحت بيحاولوا طبعا من الشهر الثامن أو التاسع
+
+839
+01:15:21,860 --> 01:15:27,360
+يديروا يخلوا السيد تلعب رياضة من .. من الرياضة
+
+840
+01:15:27,360 --> 01:15:32,680
+مثلا أنه ترمى كابريتا و تلقطها بالعود بيقولك هذا
+
+841
+01:15:32,680 --> 01:15:37,520
+ممكن يؤدي إلى أنه ينعدل إذا انعدل انعدل ما نعدلش
+
+842
+01:15:37,520 --> 01:15:40,680
+بيضطروا أنهم يعملوا لها إيش قيصرية
+
+843
+01:15:43,740 --> 01:15:46,660
+ده السجود و الركوع بتأدي لإنسانين اللي .. إن يعدل
+
+844
+01:15:46,660 --> 01:15:51,700
+اه .. احنا قاعدين بنقول إيش الأجانب بيعملوا .. ما
+
+845
+01:15:51,700 --> 01:15:53,860
+بيقولك إيش الأجانب بيعملوا، بيقول بيبقى بيبقى بيبقى
+
+846
+01:15:53,860 --> 01:15:56,720
+بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى
+
+847
+01:15:56,720 --> 01:15:56,880
+بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى
+
+848
+01:15:56,880 --> 01:15:57,000
+بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى
+
+849
+01:15:57,000 --> 01:16:09,260
+بيبقى بيبقى بيبقى بيبق
+
+850
+01:16:13,970 --> 01:16:16,870
+شهرين و هي تلعب كل أنواع الرياضة و بتصلي يعني
+
+851
+01:16:16,870 --> 01:16:20,830
+تجتاش ولا صلاة زي ما بقوله و مع ذلك لآخر دقيقة و
+
+852
+01:16:20,830 --> 01:16:25,450
+هم تايس مش راضي اللي يظلوا قاعدين يهديك يرضيك أبدا
+
+853
+01:16:25,450 --> 01:16:32,030
+فبناء عليه يعني لما يكون في وضع ما يسمحش أنه ينزل
+
+854
+01:16:32,030 --> 01:16:35,630
+واحدة ثانية بتلاقي الخلاصة نازلة في طريق الميلاد
+
+855
+01:16:38,000 --> 01:16:43,100
+الخلاصة سحل ونزل وين؟ بين الراس وطريق الميلاد طبعا
+
+856
+01:16:43,100 --> 01:16:49,780
+هذه الست ممنوع حتى تنفحص تصوروا؟ لو الدكتور بس مد
+
+857
+01:16:49,780 --> 01:16:53,780
+إصبعها أو الدكتورة مدت إصبعها ممكن تنفجر الخلاصة
+
+858
+01:16:53,780 --> 01:17:01,160
+وتخش في نزيف شديد جدا فما بدهاش مجال هذه أنه إيش؟ أنه
+
+859
+01:17:01,160 --> 01:17:06,260
+تطلق عشان هيك بنقول المتابعة في أثناء فترة الحمل
+
+860
+01:17:07,060 --> 01:17:12,000
+مهم جدا كيف بدك تعرف إن هذه الست بدها يعني قيصرية
+
+861
+01:17:12,000 --> 01:17:16,980
+ولا بدهاش؟ بهيك شغلة فقبل ما ييجي معاد ولدتها هذي
+
+862
+01:17:16,980 --> 01:17:22,140
+بيومين تلاتة بتروح المستشفى و بنعمللها إيش ما يسمى
+
+863
+01:17:22,140 --> 01:17:27,260
+بالقيصرية إيش كمان فكرة كوحالات؟
+
+864
+01:17:27,260 --> 01:17:34,680
+من وين بده ينزل الولد؟ أو البنات؟ بالظبط من الحوض
+
+865
+01:17:35,370 --> 01:17:41,810
+فيه بيكون الحوض فتحة الحوض لا تكفي لنزول الرأس رأس
+
+866
+01:17:41,810 --> 01:17:46,710
+الجنين تبقى أكبر من اللي هو إيش الحوض فهذه ما بتقدر
+
+867
+01:17:46,710 --> 01:17:51,510
+تنزل لا يمكن تحت أي ضرر من الظروف إنه اللي هي
+
+868
+01:17:51,510 --> 01:17:58,920
+تنزل فالرأس والحوض غير متوائمين رأسه أكبر من الحوض
+
+869
+01:17:58,920 --> 01:18:03,340
+و المكان ما فيش إمكانية إنه ينزل فبتنزل كمان إيش
+
+870
+01:18:03,340 --> 01:18:12,320
+ممكن من الحالات اللي بتحتاج قيصرية؟ مين ذاكرة؟
+
+871
+01:18:12,320 --> 01:18:16,180
+والله
+
+872
+01:18:16,180 --> 01:18:20,760
+بيحاولوا معاها اللي فوق ال 35 و 40 سنة ممكن يحاولوا
+
+873
+01:18:20,760 --> 01:18:26,020
+معاها هذا في البكري طبعا أما إذا كان ولدت قبل هيك
+
+874
+01:18:26,020 --> 01:18:30,820
+يخلص يعني من التلد إذا كان الست عملت قيصريتين قبل
+
+875
+01:18:30,820 --> 01:18:35,540
+هيك واخدين بالكوا كيف؟ إذا الست عملت قيصرية 
+
+876
+01:18:35,540 --> 01:18:41,520
+بيحاولوا معها تلد طبيعي إذا ولدت طبيعي معناته
+
+877
+01:18:41,520 --> 01:18:46,400
+تعدينا المشكلة لكن إذا انفتح الرحم مرتين بالمشرط 
+
+878
+01:18:46,400 --> 01:18:52,360
+ولا واحد في العالم بيجرؤ يخليها تلد المرة التالتة
+
+879
+01:18:52,360 --> 01:18:53,060
+ولدت طبيعي
+
+880
+01:18:56,030 --> 01:19:01,870
+أنا إيش قاعد بقول؟ أنا إيش قاعد بقول؟ إذا ولدت 
+
+881
+01:19:01,870 --> 01:19:06,910
+مرتين قيصرية فما فيش واحد في المرة التالتة في الدنيا
+
+882
+01:19:06,910 --> 01:19:12,190
+بيحاول أن يولدها ولادة طبيعية، ليش؟ لأنه بكل بساطة
+
+883
+01:19:12,190 --> 01:19:16,670
+ممكن ينفجر الرحم بعملية الطلق، فبنخليهاش تطلق، عشان
+
+884
+01:19:16,670 --> 01:19:20,590
+هيك بتبعت القيصرية هاد، بنقول لأ، جاب المعادة 
+
+885
+01:19:20,590 --> 01:19:25,520
+بسبوع بتيجي عن المستشفى مين اللي بتقول خالتك ولادة
+
+886
+01:19:25,520 --> 01:19:30,440
+كام مرة و اكتشفت روح المستشفى، جاب للمعادة، جاب
+
+887
+01:19:30,440 --> 01:19:35,320
+لها تبدأ تطلق، ما نسمعش أنها تطلق، لأن الطلق ضغط
+
+888
+01:19:35,320 --> 01:19:40,500
+على الرحم و الرحم مفتوح مرتين، فضعيف، فممكن جدا ..
+
+889
+01:19:40,500 --> 01:19:44,140
+ممكن ينفجر، ما فيش جرعة
+
+890
+01:19:48,180 --> 01:19:52,840
+طب عن بنجة لازم تتبنج عن بنجة طبيعي لأ بتتبنج بنجة
+
+891
+01:19:52,840 --> 01:19:57,660
+طبيعي عادي يعني لكن ما بسمحش للست أنه هذه الست يعني
+
+892
+01:19:57,660 --> 01:20:02,020
+تطلق طلقة طبيعي لأنه هي انفجر الرحم وإذا انفجر
+
+893
+01:20:02,020 --> 01:20:07,080
+الرحم مش هتلحق لا هي ولا ابنها يصلوا إلى قط
+
+894
+01:20:07,080 --> 01:20:13,300
+العمليات وبناء عليها إذا عملوا قيصريتين لا يجرؤ
+
+895
+01:20:13,300 --> 01:20:21,630
+أحد مطلقا أن يفكر يعمل ولادة طبيعية بعدين في
+
+896
+01:20:21,630 --> 01:20:27,410
+عندكوا كمان أي سؤال؟ نعم أما يجب أن يعملوا في حالة
+
+897
+01:20:27,410 --> 01:20:31,770
+ولادة ولادة مزيفة ومفردة وقبل تنزلها تقضي الظهر
+
+898
+01:20:31,770 --> 01:20:35,950
+الذين يتقبلت تقضي الظهر الذين يتقبلت أو عادي لكي
+
+899
+01:20:35,950 --> 01:20:43,110
+يتوقف الشباب عندهم ويخرجوا من هناك إيش رأيك؟ إحنا 
+
+900
+01:20:43,110 --> 01:20:48,610
+لما ينزل ظهره نقول هذه ولادة مش طبيعية فبنغطي
+
+901
+01:20:48,610 --> 01:20:50,810
+الجنين و بنوديه على المستشفى هو في بطن أمه دي
+
+902
+01:20:50,810 --> 01:20:56,070
+ربالك ارجع أجل السواد كويس أهم إيه يا عزيزي في
+
+903
+01:20:56,070 --> 01:21:00,130
+حالة ولادة .. ولادة مجيبة مقعد أو قبل فروز لرقص
+
+904
+01:21:00,130 --> 01:21:04,870
+يعني إيش؟ يعني أصلا ما نزلش اه اه أصلا اه بدك تغطيه
+
+905
+01:21:04,870 --> 01:21:08,950
+و توديه على المستشفى ماشي؟ أو تغطيه و تضمن الجنين
+
+906
+01:21:08,950 --> 01:21:14,040
+في مطاق دفتر أو تغطيه في مطاق برد أو عادي لأ دق
+
+907
+01:21:14,040 --> 01:21:17,740
+طاقعه هو أنت لما تحط على حالك بطنية هو طلع في
+
+908
+01:21:17,740 --> 01:21:23,640
+الجنيه مش هو طلع ضهره بوجهه طب السؤال تاني طبعا ..
+
+909
+01:21:23,640 --> 01:21:29,160
+اه .. بعدين بقى بدافي ولا دافي السؤال اللي مطرح
+
+910
+01:21:29,160 --> 01:21:31,800
+نفسه لما تكوني سجانة و تحطها على حالك إحرام
+
+911
+01:21:31,800 --> 01:21:37,200
+الإحرام دافي ولا بارد؟ عادي، دافي، بدفيك يعني
+
+912
+01:21:37,200 --> 01:21:41,660
+فالإحرام بارد يعني معناته بدك تجيب تلاجة، ما بحطش أنا
+
+913
+01:21:41,660 --> 01:21:46,310
+حاجة باردة يعني هو الإحرام ربنا خالقه ودافي تلبس
+
+914
+01:21:46,310 --> 01:21:52,190
+الإحرام و تدفي أهم ثورة .. ثورة .. ثورة .. ثورة ..
+
+915
+01:21:52,190 --> 01:21:53,670
+ثورة .. ثورة .. ثورة .. ثورة .. ثورة .. ثورة ..
+
+916
+01:21:53,670 --> 01:21:54,730
+ثورة .. ثورة .. ثورة .. ثورة .. ثورة .. ثورة ..
+
+917
+01:21:54,730 --> 01:21:55,010
+ثورة .. ثورة .. ثورة .. ثورة .. ثورة .. ثورة ..
+
+918
+01:21:55,010 --> 01:21:55,570
+ثورة .. ثورة .. ثورة .. ثورة .. ثورة .. ثورة ..
+
+919
+01:21:55,570 --> 01:21:56,050
+ثورة .. ثورة .. ثورة .. ثورة .. ثورة .. ثورة ..
+
+920
+01:21:56,050 --> 01:22:00,350
+ثورة .. ثورة .. ثورة .. ثورة .. ثورة .. ثورة ..
+
+921
+01:22:00,350 --> 01:22:04,410
+ثورة .. ثورة .. ثورة .. ثورة .. ثورة .. ثورة ..
+
+922
+01:22:04,410 --> 01:22:12,290
+ثورة .. ثورة
+
+923
+01:22:12,290 --> 01:22:19,220
+.. ثورة اه سابق بمنعش الولادة إحنا
+
+924
+01:22:19,220 --> 01:22:24,500
+قاعدين بيقولك بتقدر تمنعي؟ إحنا قاعدين إيش بيقول؟
+
+925
+01:22:24,500 --> 01:22:35,160
+قاعدين بيقول تمنعيش، تطلق تلد هي خلاص، اه
+
+926
+01:22:35,160 --> 01:22:42,330
+كله غلط هذا سؤال ما فيش منه أنت قاعد إيش بتقول يجري
+
+927
+01:22:42,330 --> 01:22:48,190
+السؤال تاني جايب المقعدة جايب المقعدة معناته ولادة
+
+928
+01:22:48,190 --> 01:22:57,170
+مش طبيعية نقطة علشان بدك تشدي اجري البدائل الفك
+
+929
+01:22:57,170 --> 01:23:00,650
+السفلي للمولود جايب مجعلته إيش خاصة الفك السفلي
+
+930
+01:23:00,650 --> 01:23:07,130
+اللي محطته فوق بعده الفك العلوي إيش .. كيف بتصلي
+
+931
+01:23:07,130 --> 01:23:12,340
+الفك العلوي بعده الرأس فوق فوق، الكتب فوق فوق قدمان
+
+932
+01:23:12,340 --> 01:23:15,360
+يعني، مين كان قدامك؟ جايب بالمقعدة، كبدك تشدي،
+
+933
+01:23:15,360 --> 01:23:18,220
+ما خلصناش أنا هاد الولادة غير طبيعي و بودع المستشفى
+
+934
+01:23:18,220 --> 01:23:29,260
+بالظبط، هاد ما خلصنيش، قولي اه، سؤال مش غيره تفضل
+
+935
+01:23:29,260 --> 01:23:37,000
+نعم، مين عندها سؤال؟ عندك أسئلة؟ عندك أسئلة؟ 
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/3_y6SgyBxoo.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/3_y6SgyBxoo.srt
new file mode 100644
index 0000000000000000000000000000000000000000..882938e63c8394d2ab371aace9d8702406bdc02e
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/3_y6SgyBxoo.srt
@@ -0,0 +1,2397 @@
+1
+00:00:20,940 --> 00:00:23,200
+بسم الله الرحمن الرحيم احنا كنا بنقول إن ال ..
+
+2
+00:00:23,200 --> 00:00:26,620
+العنوان المحاضرة هو عبارة عن bacterial morphology
+
+3
+00:00:26,620 --> 00:00:30,960
+and cell structure وتحدثنا عن أن البكتيريولوجي
+
+4
+00:00:30,960 --> 00:00:34,320
+أو المايكروبيولوجي كانوا يستخدموهم ك synonymous يعني
+
+5
+00:00:34,320 --> 00:00:36,600
+وكأنه هذا المعنى بيدل على هذا المعنى، معانيهم
+
+6
+00:00:36,600 --> 00:00:39,720
+مختلفين البكتيريولوجي هو جزء من المايكروبيولوجي
+
+7
+00:00:40,160 --> 00:00:44,760
+ولكن كون هو الدُّزق الأكبر فكان يعرف الكل بالدُّزق
+
+8
+00:00:44,760 --> 00:00:49,960
+الأكبر تمام؟ فكان يعرف بهذا الشكل وبتالي هتلاقوا
+
+9
+00:00:49,960 --> 00:00:52,820
+أن معظم كتب الميكروبيولوجي بتركز على البكتيريال
+
+10
+00:00:52,820 --> 00:00:56,380
+cell structure أكثر من الفنجاي أكثر من الفيروسات
+
+11
+00:00:56,380 --> 00:00:58,660
+وبيعطوها أهمية أكبر من الحجم الأكبر جدًا
+
+12
+00:00:58,660 --> 00:01:03,180
+للبكتيريولوجيا كنا بنعرف قبل اليوم مقاييسنا
+
+13
+00:01:03,180 --> 00:01:09,190
+بالمتر وبالسنتيمتر و بالمليمتر هذا قدرتنا على ..
+
+14
+00:01:09,190 --> 00:01:13,150
+قدرة العين تبعتنا على تمييز بين نقطتين مختلفتين حد
+
+15
+00:01:13,150 --> 00:01:18,670
+ما نسميها resolution قدرة
+
+16
+00:01:18,670 --> 00:01:23,550
+تمييز العين على نقطتين علينهم نقطتين وانفصلتهم
+
+17
+00:01:23,550 --> 00:01:27,450
+نسميها ال resolution power ويمكن نقلعته في
+
+18
+00:01:27,450 --> 00:01:31,350
+الميكروسكوبي .. في الميكروسكوب الضوئي في العدسات
+
+19
+00:01:31,350 --> 00:01:32,430
+اللي هيبقى resolution
+
+20
+00:01:35,730 --> 00:01:39,970
+وأذكركم بأن الـ size اللي احنا بنشوفه مليميتر صغير
+
+21
+00:01:39,970 --> 00:01:46,090
+خالص فبالك لو بنتكلم عن جزء من الف من المليميتر
+
+22
+00:01:46,090 --> 00:01:52,050
+اللي هو ال micro meter لن نراه لن نراه طيب لو كان
+
+23
+00:01:52,050 --> 00:01:56,770
+جزء من عشر أس ناقص ستة مش عشر أس ناقص ثلاثة عشر أس ناقص ستة
+
+24
+00:01:56,770 --> 00:02:00,990
+يعني واحد من مليون من المليميتر لن نراه أبدا حتى
+
+25
+00:02:00,990 --> 00:02:04,470
+بالميكروسكوب العادي إذا نعينا مليمتر
+
+26
+00:02:05,290 --> 00:02:09,610
+المايكروسكوب العادي ميكرونات ميكرون مايكروميتر
+
+27
+00:02:09,610 --> 00:02:13,210
+أو ميكرون نفس المعنى مايكروميتر وهو عشر أس ناقص
+
+28
+00:02:13,210 --> 00:02:17,810
+ثلاث يعني واحد على ألف واحد على ألف ال nanometer
+
+29
+00:02:17,810 --> 00:02:21,930
+واحد على عشر أس ستة أو عشر أس ناقص ستة يعني
+
+30
+00:02:21,930 --> 00:02:24,950
+وبالتالي واحد على مليون واحد على مليون إذا ال
+
+31
+00:02:24,950 --> 00:02:29,010
+nanometer أصغر من مين؟ من ال micro meter البكتيريا
+
+32
+00:02:29,010 --> 00:02:32,290
+تبعتنا غالبًا بنجيزها بال micro meter بال micro
+
+33
+00:02:32,290 --> 00:02:36,080
+meter وبالتالي نستطيع رؤيتها بال light microscope
+
+34
+00:02:36,080 --> 00:02:41,700
+لكن دون تفاصيل، دون تفاصيل، ليش؟ لأن التفاصيل
+
+35
+00:02:41,700 --> 00:02:46,020
+بتكون بال nano meter فتحتاج إلى microscope أقوى
+
+36
+00:02:46,020 --> 00:02:49,320
+اللي هو ال electron microscope، إذا ال size بيكون
+
+37
+00:02:49,320 --> 00:02:53,980
+شعور بالحجم، نتكلم عن millimeter، وين جدش ال
+
+38
+00:02:53,980 --> 00:02:57,580
+millimeter؟ جزء من الظفر تبعتنا، اللي من يدوك
+
+39
+00:02:57,580 --> 00:03:02,440
+بنشوفه، مصبوط؟ تخيلوا أصغر من هذا الجزء بـ 1000 مرة
+
+40
+00:03:03,990 --> 00:03:07,670
+يبقى بدنا يكون هنا sense of feeling ليه الحجوم و
+
+41
+00:03:07,670 --> 00:03:12,670
+هنشوفه كمان شوية إن شاء الله الكلام عن ال size و
+
+42
+00:03:12,670 --> 00:03:15,330
+أن ال size صغير و ايش الأرقام اللي أتعامل معاها
+
+43
+00:03:15,330 --> 00:03:18,130
+الآن نتكلم عن ال morphology ده لاحظتوا يا عزيزي في
+
+44
+00:03:18,130 --> 00:03:22,550
+الابتدائي أو الإعدادي أن البكتيريا إما كروية أو
+
+45
+00:03:22,550 --> 00:03:26,630
+عصوية أو spiral اللي هي بيقولوا عليها بكتيريا بسلار أو
+
+46
+00:03:26,630 --> 00:03:31,590
+spiral لكن اليوم بدنا ناخد شوية تفاصيل it is not
+
+47
+00:03:31,590 --> 00:03:34,570
+only the morphology that is matter كمان ال
+
+48
+00:03:34,570 --> 00:03:37,650
+arrangement تبعت الخلايا ال arrangement لحظوا هنا
+
+49
+00:03:37,650 --> 00:03:40,850
+في خليتين جانب بعض ملازمين بعض في أنواع من
+
+50
+00:03:40,850 --> 00:03:45,230
+البكتيريا بنشوفها دائمًا بهذا الشكل فكويس إن نعرف
+
+51
+00:03:45,230 --> 00:03:49,690
+احنا قلنا في التعريف عملية استثناء مين مش لابس
+
+52
+00:03:49,690 --> 00:03:53,530
+نظارة فاكرين المحاضرة السابقة كانت عملية استثناء
+
+53
+00:03:53,530 --> 00:03:57,690
+مين من الخلايا لا يمتلك هذه الصفة أو مين من الخلايا
+
+54
+00:03:57,690 --> 00:04:01,410
+لا يمتلك هذه الصفة خدوا بالكم نيجاتيف أو positive
+
+55
+00:04:01,410 --> 00:04:08,430
+بالنسبة إن هي صفة وتستخدم في التعريف بمعنى تمتلك
+
+56
+00:04:08,430 --> 00:04:12,550
+المقدرة على إنتاج إنزيم X أو لا تمتلك المقدرة على
+
+57
+00:04:12,550 --> 00:04:17,410
+إنتاج إنزيم X هي صفة سواء صفة سلبية أو صفة إيجابية
+
+58
+00:04:17,410 --> 00:04:21,110
+تعتبر صفة وتستخدم في عملية اللي هو الاستثمار
+
+59
+00:04:21,110 --> 00:04:27,130
+دبلوكوكاي ليه هما تنتهي مع بعض دبلوكوكساي في عندي
+
+60
+00:04:27,130 --> 00:04:31,930
+سلسلة يسموها ستربتوكوكاي
+
+61
+00:04:32,550 --> 00:04:37,910
+في عندي tetrads اللي هي الأربعات Tetrads
+
+62
+00:04:37,910 --> 00:04:44,550
+وفي عندي اللي هو ال clusters أو العناقيد وتسمى
+
+63
+00:04:44,550 --> 00:04:48,650
+Staphylococcus
+
+64
+00:04:48,650 --> 00:04:54,290
+طب هنضيف لها كلمة Staphylococci Tetrads مالهاش للأسف
+
+65
+00:04:54,290 --> 00:04:58,030
+يعني ما بينفعش معها هذا المقطع لكن ستربتوكوكاي
+
+66
+00:04:58,030 --> 00:05:02,080
+و Diplococci بينفع مع هذا المقطع هنلاقي حالنا لو
+
+67
+00:05:02,080 --> 00:05:06,160
+بتكلم على شكل واحد، arrangement مختلفة، أعطتنا
+
+68
+00:05:06,160 --> 00:05:11,240
+أسماء مختلفة، أعطتنا أنواع بكتيريا مختلفة، إذًا،
+
+69
+00:05:11,240 --> 00:05:17,460
+Size، شكل، Arrangement، نيجي للبسلالي، ال rods
+
+70
+00:05:17,460 --> 00:05:21,080
+عندي، في شاف تلاحظين، single cell، خلية لوحدها،
+
+71
+00:05:21,080 --> 00:05:25,220
+مافيش أي ارتباط مع خلايا أخرى، هنلاقيها واحدة تانية،
+
+72
+00:05:25,220 --> 00:05:29,760
+نوع تاني، اتنين، دبلو بسلالي المرة دي، مش دبلو
+
+73
+00:05:29,760 --> 00:05:33,850
+كوكاية واحد اتنين تلاتة أربعة إذا كنا بمين بال
+
+74
+00:05:33,850 --> 00:05:38,570
+ستربتو سميناها ستربتو باسيللي هذه حاجة unique هي
+
+75
+00:05:38,570 --> 00:05:44,710
+دبلة كوكاي ولا دبلة باسيللي ولا باسيللي ولا كوكاية
+
+76
+00:05:44,710 --> 00:05:50,910
+ماخدة شكل بين الاتنين وتسمى كوكو باسيللي يعني هي
+
+77
+00:05:50,910 --> 00:05:54,970
+باسيللي لكن ماخدة شكل كوكساية أو كوكساية ماخدة شكل
+
+78
+00:05:54,970 --> 00:06:00,980
+باسيللي لكن مدرسينها تحت ال باسيللي يبقى في تصنيفها
+
+79
+00:06:00,980 --> 00:06:06,900
+تنتمي لمين؟ للبسلين طبعًا ليش؟ لأن ال structure تبع
+
+80
+00:06:06,900 --> 00:06:12,180
+ال cell wall تبعها أقرب إلى البسلين من الكوكسر في
+
+81
+00:06:12,180 --> 00:06:15,840
+العمود الأخير فينا ال spiral ال spiral ده بتلاحظوا
+
+82
+00:06:15,840 --> 00:06:20,100
+الخلية هذه اللي قدامكم كام turn فيها كام لف يعني
+
+83
+00:06:20,100 --> 00:06:25,720
+واحد اثنين ثلاثة هاي الأكواس تبعتها طب تعالوا
+
+84
+00:06:25,720 --> 00:06:31,460
+تشوفوا التاني هذا تحتها واحد اتنين، ثلاثة، أربعة،
+
+85
+00:06:31,460 --> 00:06:37,580
+خمسة، ستة، سبعة، ثمانية، الكتير التنتين تم تصنيفهم
+
+86
+00:06:37,580 --> 00:06:42,160
+على أنهم إيش Spiral طب هذه لف واحد اللي هي
+
+87
+00:06:42,160 --> 00:06:44,640
+البكتيريا اللي درستوها أكيد في المدرسة باسم
+
+88
+00:06:44,640 --> 00:06:50,160
+البكتيريا الضمية أو الوعوية اللي هي ال Vibrio خد
+
+89
+00:06:50,160 --> 00:06:57,890
+بالكوا شكلاً هذه بنصنفها تحت ال spiral شكلاً لكن طبياً
+
+90
+00:06:57,890 --> 00:07:01,490
+مصنفها الأحمين تحت ال Basel Light لأن هي الأقرب في
+
+91
+00:07:01,490 --> 00:07:04,330
+الشكل وفي التركيب لميه مرضه لل Basel Light لكن
+
+92
+00:07:04,330 --> 00:07:08,990
+Basel Light منحنى .. Basel Light منحنى طيب سنحنا
+
+93
+00:07:08,990 --> 00:07:14,150
+الآن تلت شغلات مهمة جدًا .. إيش هما؟ واحد .. Size
+
+94
+00:07:14,150 --> 00:07:17,130
+.. وقولوا بالكم وأنا سألكوا سؤال بسيط جدًا .. و
+
+95
+00:07:17,130 --> 00:07:20,030
+أنتم صغار .. وأنتم صغار .. والأكبر .. لو
+
+96
+00:07:20,030 --> 00:07:23,450
+جسدكم بالمتر وأنتم صغار .. هتعطوا مقياس مختلف
+
+97
+00:07:23,450 --> 00:07:27,840
+.. مع أنكم human .. من وجهة النظر لو بدى انجليزكم
+
+98
+00:07:27,840 --> 00:07:31,560
+بنقول إن your size is age dependent، age
+
+99
+00:07:31,560 --> 00:07:33,800
+dependent، معتمدة على العمر، والبكتيريا كذلك
+
+100
+00:07:33,800 --> 00:07:38,200
+العمر، قد تكون البكتيريا صغيرة في العمر أو قد تكون
+
+101
+00:07:38,200 --> 00:07:43,580
+كبيرة في العمر فال size بها يختلف age رقم اتنين،
+
+102
+00:07:43,580 --> 00:07:48,180
+لو احنا حطينا البكتيريا في nutrient rich media،
+
+103
+00:07:48,180 --> 00:07:53,650
+وسط غني بالغذاء ونفس البكتيريا نسخة منها حقناها في
+
+104
+00:07:53,650 --> 00:07:57,310
+nutrient poor media هل تعتقد إن ال size تبقى
+
+105
+00:07:57,310 --> 00:08:01,630
+معاكم واحد؟ إن ال size is a variable يعني age
+
+106
+00:08:01,630 --> 00:08:05,030
+dependent و nutrient dependent طب ليش nutrient
+
+107
+00:08:05,030 --> 00:08:09,670
+dependent؟ البكتيريا بدها أكسجين ووفرنا لهاش احنا
+
+108
+00:08:09,670 --> 00:08:13,290
+كمية كافية من الأكسجين برضه إنهم تبقى حاجبين أو
+
+109
+00:08:13,290 --> 00:08:20,610
+حاجة تبقى حاجبين أصغر بعدين shape وبعدين cell. اللي
+
+110
+00:08:20,610 --> 00:08:24,090
+بيهمني الصحيح أنا إن أنا أعرف إن البكتيريا بكل
+
+111
+00:08:24,090 --> 00:08:30,450
+بساطة تحت ال microscope can exhibit one of the
+
+112
+00:08:30,450 --> 00:08:33,270
+three forms اللي هم بيقولوا عنهم ال bauxite و
+
+113
+00:08:33,270 --> 00:08:37,270
+البيسلاج و ال spiral كمان شغل مهم تانية إن ال
+
+114
+00:08:37,270 --> 00:08:42,210
+arrangement هل هي ديبلوما ولا ستربتو ولا اللي هي
+
+115
+00:08:42,210 --> 00:08:46,550
+الاستفالة وكده أو بالنسبة للبيسلاج الجزء الأهم في
+
+116
+00:08:46,550 --> 00:08:50,630
+محاضرتنا اليوم هو البثيلة للسلسلة وأن أرجو إن
+
+117
+00:08:50,630 --> 00:08:53,430
+تركزوا معاه كويس لأن المحاضرة مسجلة وبتالي
+
+118
+00:08:53,430 --> 00:08:57,110
+هتقعدوها مرة تانية وثالثة وال presentation هتكون
+
+119
+00:08:57,110 --> 00:09:00,310
+available وروشية الله على ال group تبعتنا في ال
+
+120
+00:09:00,310 --> 00:09:07,970
+facebook احنا في قاعة في internal stuff يعني طولات
+
+121
+00:09:07,970 --> 00:09:12,350
+وخزانات بالادمين كاميرات وفي هنا جدار وورا
+
+122
+00:09:12,350 --> 00:09:17,370
+الجدار قد يكون هناك أشياء أخرى في الخلية تبعدناها
+
+123
+00:09:17,370 --> 00:09:21,770
+اللي احنا قاعدين فيها بنقسم الخلية البكتيرية إلى
+
+124
+00:09:21,770 --> 00:09:26,370
+internal و external structures اللي مفصل بينهم مين
+
+125
+00:09:26,370 --> 00:09:31,670
+الجدار هذا المواضيع تحدثنا على أن البروكايوت تمتلك
+
+126
+00:09:31,670 --> 00:09:37,710
+جدار جدار البكتيريا ما يميزه هو وجود structure
+
+127
+00:09:37,710 --> 00:09:43,070
+اسمه بيبtidoglycan لا يوجد في العالم مخلوق غير 
+
+128
+00:09:43,070 --> 00:09:48,980
+البكتيريا على حد علمنا عنده بيبtidoglycan إلا
+
+129
+00:09:48,980 --> 00:09:52,920
+البكتيريا يعني النباتات إليها cell wall لكن
+
+130
+00:09:52,920 --> 00:09:55,480
+النباتات مكوّنة ال cell wall تبقى حالمين من
+
+131
+00:09:55,480 --> 00:10:00,540
+cellulose الفطريات إليها برضه cell wall لكن بيختلف
+
+132
+00:10:00,540 --> 00:10:04,440
+في التركيب عن النباتات وعن اللي هو البكتيريا
+
+133
+00:10:04,440 --> 00:10:07,700
+فالبكتيريا ما يميزها ال بيبtidoglycan اتفقنا
+
+134
+00:10:07,700 --> 00:10:11,500
+إنه في internal وفي external تعالوا نطلع على ال
+
+135
+00:10:11,500 --> 00:10:15,640
+external إيش طالع من ال cell wall هنا؟ Flagella
+
+136
+00:10:16,190 --> 00:10:21,390
+اللي هي الأسواط طالع منه كمان حاجة اسمها المحفظة
+
+137
+00:10:21,390 --> 00:10:24,850
+اللي سمتوها أنتو باللغة العربية سميناها capsule
+
+138
+00:10:24,850 --> 00:10:30,050
+طالع منها كمان fimbriae اللي هي الزوائد اللي
+
+139
+00:10:30,050 --> 00:10:33,950
+شايفينها هذه الخضرة كمان إيش سميناها دي بالراحة
+
+140
+00:10:33,950 --> 00:10:38,410
+ليست organ motility ليست لها علاقة بالحركة وإنما
+
+141
+00:10:38,410 --> 00:10:42,160
+لها علاقة بالحركة من؟ الفلاجيلات الـ Capsule اللي هو
+
+142
+00:10:42,160 --> 00:10:44,600
+ضايف على تراكيبها و الـ ... و الـ Pili برضه اللي هو
+
+143
+00:10:44,600 --> 00:10:49,280
+ضايف لكن نعرف هي التراكيب Pili Flagella زي اللي هو
+
+144
+00:10:49,280 --> 00:10:54,000
+الـ Capsule نجي للداخل إيش أول حاجة بعد ال cell
+
+145
+00:10:54,000 --> 00:10:59,300
+wall من جوا؟ البوية الدهان اللي هو ال cell
+
+146
+00:10:59,300 --> 00:11:04,540
+membrane طبقة رقيقة طبقة رقيقة من ال lipids من ال
+
+147
+00:11:04,540 --> 00:11:08,100
+lipids وفيها شوية proteins فيها شوية proteins
+
+148
+00:11:08,100 --> 00:11:12,420
+بسموها cell membrane أو cytoplasmic membrane درستوها بعدين
+
+149
+00:11:12,420 --> 00:11:16,660
+جوا فيه cytoplasm داخل الخلية في cytoplasm في ال
+
+150
+00:11:16,660 --> 00:11:21,800
+cytoplasm في بعض المكونات أهمها ال chromosome اللي
+
+151
+00:11:21,800 --> 00:11:25,160
+جاية في منطقة النواة اللي بيسموها ما يشبه النواة
+
+152
+00:11:25,160 --> 00:11:29,580
+Nucleoid لأنها مش نواة حقيقية فمنها Nucleoid وفيها
+
+153
+00:11:29,580 --> 00:11:33,820
+ال chromosome اللي قلنا عنه one circular single
+
+154
+00:11:33,820 --> 00:11:38,680
+chromosome إيش تاني اللي عاملين الـ ...الريبوزومات
+
+155
+00:11:38,680 --> 00:11:42,460
+اللي حكينا عنها المرة الماضية إنهم 70S، خمسين،
+
+156
+00:11:42,460 --> 00:11:46,520
+ثلاثين، فاكرين؟ وفي granules باللون الأزرق هنا أو
+
+157
+00:11:46,520 --> 00:11:50,420
+البرملي حجم granules، الـ granules زي ما إحنا
+
+158
+00:11:50,420 --> 00:11:53,280
+عندنا طلعات في البيت، بنحاول نخزن الأكل لسبوع أو
+
+159
+00:11:53,280 --> 00:11:57,260
+أسابيع، البكتيريا بتحاول تخزن بعض أنواع الأغذية أو
+
+160
+00:11:57,260 --> 00:12:03,500
+بعض المواد لازمها لأغراض محددة بتخزينها في حبيبات
+
+161
+00:12:03,500 --> 00:12:08,120
+بيسميها inclusion bodies تمام؟ أو granular
+
+162
+00:12:08,120 --> 00:12:13,680
+inclusion حبيبات جنة تخزينية إذا صار عندنا فكرة عن
+
+163
+00:12:13,680 --> 00:12:17,780
+التراكيب الخارجية والتراكيب الداخلية بس فيها تركيب
+
+164
+00:12:17,780 --> 00:12:22,440
+مش ذاكرة في التدرس ما حال اسمها شكل دائري حال
+
+165
+00:12:22,440 --> 00:12:28,500
+اسمها plasma هل هتطلع تقولها بشكل مبصر؟ لأ اتفقنا
+
+166
+00:12:28,500 --> 00:12:32,600
+على التراكيب الداخلية والتراكيب الخارجية للخلية
+
+167
+00:12:32,600 --> 00:12:36,020
+تصنيف
+
+168
+00:12:36,020 --> 00:12:41,960
+غير اللي اتكلمنا فيه اللي هو داخلي وخارجي فيه
+
+169
+00:12:41,960 --> 00:12:45,620
+essential component وفيه special structures تراكيب
+
+170
+00:12:45,620 --> 00:12:50,460
+خاصة غالبا هذه بدونها الخلية لا تستطيع المعيشة
+
+171
+00:12:50,460 --> 00:12:54,330
+البكتيرية هنا ممكن تعيش بدون هذه التراكيب زي
+
+172
+00:12:54,330 --> 00:12:57,790
+الكبسول والـ fimbriae والـ Pili والـ spores لكن بدون
+
+173
+00:12:57,790 --> 00:13:01,750
+cell wall صعب جدا بدون cell membrane صعب بدون
+
+174
+00:13:01,750 --> 00:13:05,590
+cytoplasm صعب مستحيل بدون nuclear material أيضا
+
+175
+00:13:05,590 --> 00:13:09,630
+مستحيل وصلت الصورة إذا صنفناهم على تراكيب أساسية
+
+176
+00:13:09,630 --> 00:13:15,430
+ضرورية وتراكيب ممكن تكون خاصة
+
+177
+00:13:15,430 --> 00:13:20,330
+توقفوا اللوحة وركزوا معايا اللي بتتكلمه الآن هو
+
+178
+00:13:20,330 --> 00:13:23,450
+الأهم على الإطلاق في المحاضرة الجزء اللي أنا
+
+179
+00:13:23,450 --> 00:13:27,290
+هأركز عليه اللي هو ال cell wall وركزوا معاه كويس و
+
+180
+00:13:27,290 --> 00:13:33,150
+بناخد كتابة عشان ناخد الفكرة العامة من التركيب تبع
+
+181
+00:13:33,150 --> 00:13:36,650
+ال cell wall تبع البكتيريا اتفقنا رقم واحد إنه لا
+
+182
+00:13:36,650 --> 00:13:42,210
+يوجد مخلوقات أخرى سواء البكتيريا تحتوي على تركيب
+
+183
+00:13:42,210 --> 00:13:47,930
+اسمه peptidoglycan مما يتكون ال peptidoglycan
+
+184
+00:13:47,930 --> 00:13:58,780
+بتكون منه سكرين نختصرهم NAM و NAG NAM و NAG ناخد
+
+185
+00:13:58,780 --> 00:14:12,840
+منهم الحرفين هذول لاختصار تاني نمسح
+
+186
+00:14:12,840 --> 00:14:15,660
+الـ NAM والـ NAG ونشوف كيف التركيب
+
+187
+00:14:50,940 --> 00:14:52,340
+مجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمج
+
+188
+00:14:52,500 --> 00:14:56,540
+تكتفي بطبقة واحدة من ال peptidoglycan وأنواع أخرى
+
+189
+00:14:56,540 --> 00:15:03,140
+بدها multiple layers المشكلة في اللي فيها اللي لها
+
+190
+00:15:03,140 --> 00:15:08,320
+طبقات متعددة اللي لها طبقات متعددة ما بتقدرش تحط
+
+191
+00:15:08,320 --> 00:15:14,260
+طبقة فوق طبقة هكذا لإن بتكون التركيب ضعيف جدا لازم
+
+192
+00:15:14,260 --> 00:15:15,200
+يطلع
+
+193
+00:15:21,290 --> 00:15:26,310
+أربع amino acid وأربع amino acid من الـ NAM والـ NAG
+
+194
+00:15:26,310 --> 00:15:30,590
+اللي في الطبقتين تمام بيسموهم إيش أربع باللاتيني
+
+195
+00:15:30,590 --> 00:15:35,930
+Tetra Tetra peptide يعني ببتيد مكون من أربع amino
+
+196
+00:15:35,930 --> 00:15:42,670
+acids واضحة الصورة بين الـ NAM والـ NAG وبيربط بينهم
+
+197
+00:15:42,670 --> 00:15:51,950
+another peptide هذا الرابط بنسميه cross link cross
+
+198
+00:15:51,950 --> 00:15:59,930
+link تخيلوا ما فيش cross link الطبقة هتصير
+
+199
+00:15:59,930 --> 00:16:02,230
+collapsed على الطبقة التانية هتنزل على الطبقة
+
+200
+00:16:02,230 --> 00:16:08,870
+التانية ال cell wall فقط واضيفته فقط واضيفته وهناك
+
+201
+00:16:08,870 --> 00:16:13,170
+بعض ال antibiotics تقتل البكتيريا بأنها تمنع تكوين
+
+202
+00:16:13,170 --> 00:16:18,150
+ال cross link وصلت الصورة ليش قلتلكوا النقطة
+
+203
+00:16:18,150 --> 00:16:23,240
+المهمة؟ لأن تركيب ال cell wall بوضح لي كيف بعض ال
+
+204
+00:16:23,240 --> 00:16:26,580
+antibiotics بتشتغل وجزء من دراستنا يا بنات إحنا
+
+205
+00:16:26,580 --> 00:16:30,400
+لازم نرجع عارفين كيف ال antibiotics بتؤدي وظيفتها،
+
+206
+00:16:30,400 --> 00:16:36,020
+تمام؟ ليه المضادات الحيوية ليش بقولوا عنها
+
+207
+00:16:36,020 --> 00:16:42,300
+قاتلة للبكتيريا؟ قاتلة غالبا للبكتيريا المعتمدة
+
+208
+00:16:42,300 --> 00:16:46,350
+على طبقات متعددة من peptidoglycan 
+
+209
+00:16:46,350 --> 00:16:52,310
+لأنها بتكسر الرابط بين الـ NAM والـ NAG بتكسر الطبقة
+
+210
+00:16:52,310 --> 00:16:57,290
+تبع ال peptidoglycan لذلك لذلك معظم التهابات
+
+211
+00:16:57,290 --> 00:17:01,050
+اللي عين بتسببها الـ Gram Negative مش الـ Gram
+
+212
+00:17:01,050 --> 00:17:02,610
+Positive طبعا أنتو مش عارفين إيش Gram Negative ولا
+
+213
+00:17:02,610 --> 00:17:08,310
+Gram Positive لسه فبالتالي هنشرح الموضوع إحنا
+
+214
+00:17:08,310 --> 00:17:12,650
+قلنا بأن البكتيريا قد تكتفي بطبقة واحدة وأنواع
+
+215
+00:17:12,650 --> 00:17:17,780
+أخرى قد تكتفي بطبقات متعددة تعالوا نشوف وين فيه تطبيق
+
+216
+00:17:17,780 --> 00:17:22,100
+عملي لهذا الموضوع في عالم ألماني اسمه Christian
+
+217
+00:17:22,100 --> 00:17:26,720
+Gram عمل صبغة اسمها Gram stain ممكن دول مرض عليكم
+
+218
+00:17:26,720 --> 00:17:31,780
+اللي اسمه Gram stain الـ Gram stain عشان نكون واضحين
+
+219
+00:17:31,780 --> 00:17:35,700
+وبساطة نذكر هذا اللون violet وهذا اللون أحمر
+
+220
+00:17:35,700 --> 00:17:39,060
+أنتو Gram Negative وأنتو Gram Positive أنتو
+
+221
+00:17:39,060 --> 00:17:43,580
+ما كنوش عارفين الآن جينا صبغنا العالم الجرام صبغ
+
+222
+00:17:43,580 --> 00:17:48,260
+الاثنين بلون violet اسمه crystal violet البكتيريا
+
+223
+00:17:48,260 --> 00:17:52,820
+في الوضع الطبيعي شفافة عديمة اللون فبتنطس الصبغة
+
+224
+00:17:52,820 --> 00:17:56,980
+بسهولة وخاصة إن البكتيريا بتكون شحنتها سالبة
+
+225
+00:17:56,980 --> 00:18:00,240
+والصبغة اللي بحكي عنها crystal violet شحنتها موجبة
+
+226
+00:18:00,240 --> 00:18:03,060
+بيصير فيه نوع من ال attraction الجذب بين الاثنين
+
+227
+00:18:03,060 --> 00:18:09,040
+ف الاثنين بينصبغوا بلون violet برغم إن إحنا ضايفين
+
+228
+00:18:09,040 --> 00:18:14,310
+عليهم أيودين إضافة اليود، إيش بتعمل؟ اليود بدخل
+
+229
+00:18:14,310 --> 00:18:17,930
+حجمه صغير والـ crystal violet نوعا ما حجمه صغير
+
+230
+00:18:17,930 --> 00:18:22,590
+لكن لما اليود بيلاقي الـ crystal violet جوه بيرتبط
+
+231
+00:18:22,590 --> 00:18:27,330
+معاه وبيكوّن مركب معقد هذا المركب المعقد
+
+232
+00:18:27,330 --> 00:18:32,070
+يصعب خروجه من خلية مرة أخرى ودخل من ثقوب صغيرة وكان
+
+233
+00:18:32,070 --> 00:18:35,670
+حجمه صغير، لكن إيش صار جوه؟ صار كبير لأنه
+
+234
+00:18:35,670 --> 00:18:40,630
+مركب معقد، بطل حجمه صغير فصبغنا وبعدين ثبتنا
+
+235
+00:18:40,630 --> 00:18:45,030
+اللون بالأيودين الأيودين هنا مثبت للون
+
+236
+00:18:45,030 --> 00:18:50,850
+خطوة التالتة الحاسمة ضفنا مادة decolorizer مادة
+
+237
+00:18:50,850 --> 00:18:56,150
+مزيل لللون وهي عبارة عن إيثانول إيثانول بتعرفوه ال
+
+238
+00:18:56,150 --> 00:19:00,710
+إيثانول بينجح بإزالة اللون من ال Gram Negative ولا
+
+239
+00:19:00,710 --> 00:19:04,470
+ينجح بإزالة اللون من مين؟ من ال Gram Positive إحنا
+
+240
+00:19:04,470 --> 00:19:09,570
+ما كناش عارفين اللون الأصلي للبكتيريا شفافة يبقى هتش صار
+
+241
+00:19:09,570 --> 00:19:14,600
+فيها اللون هنا رجعت شفافة وهذه ضالة violet ضفنا
+
+242
+00:19:14,600 --> 00:19:19,680
+عليهم صبغة تانية لونها أحمر اسمها Safranin هذه
+
+243
+00:19:19,680 --> 00:19:24,080
+البكتيريا هتتذكروا إنه أزيل اللون منها فلما تجيها
+
+244
+00:19:24,080 --> 00:19:28,580
+صبغة جديدة هتختها هذه فيها صبغة لما نحطها على
+
+245
+00:19:28,580 --> 00:19:32,740
+صبغة جديدة ما هتختهاش لأنها فيها already صبغة يبقى
+
+246
+00:19:32,740 --> 00:19:36,780
+المحصلة النهائية اللي قلنا عليها لونها red ولونها violet 
+
+247
+00:19:36,780 --> 00:19:40,320
+إذا أنتو Gram Positive، فإيش اللون كم مرة؟ Violet، واللون؟ Red، Gram Negative الأمر
+
+248
+00:19:40,320 --> 00:19:46,160
+سهل لإنه قصة، أنا حددتلكوا حدوتة، قلتلكوا قصة إن
+
+249
+00:19:46,160 --> 00:19:50,580
+كيف عملنا وإيش اللي صار معاها لكن الآن نجي
+
+
+
+251
+00:19:55,040 --> 00:20:00,200
+للتفسير، لماذا البكتيريا هذه فقدت اللون اللي Gram 
+
+252
+00:20:00,200 --> 00:20:04,650
+Negative والبكتيريا الأخرى لم تفقد اللون؟ هنا
+
+253
+00:20:04,650 --> 00:20:10,790
+المُكوّن اللي مُقَدّر الـ cell wall يُفسّر لنا ليش فقدت و
+
+254
+00:20:10,790 --> 00:20:17,970
+ليش لم تفقد إلا طبعًا
+
+255
+00:20:17,970 --> 00:20:20,490
+على الصورة هذه اللي قدامنا، الصورة هذه بتقول ..
+
+256
+00:20:20,490 --> 00:20:24,990
+بتقول أنّ دي طبقات مُتعددة من ببتيدوجلايكان، تخيلوها
+
+257
+00:20:24,990 --> 00:20:30,600
+كالتالي، تعرفوا الشبك المنخل؟ حطّوا منخل وحطّوا منه ماء
+
+258
+00:20:30,600 --> 00:20:35,380
+إيش بيصير؟ بنزل طبقة، حطّوا منخلين، ثلاثة، أربعة، خمسة
+
+259
+00:20:35,380 --> 00:20:39,460
+وجرّبوا .. بتتجلّ وبيصير فيه بطء في الحركة وفي
+
+260
+00:20:39,460 --> 00:20:43,480
+صعوبة للسائل في صعوبة .. يعني ممكن ننجلّ ماء فيهم ..
+
+261
+00:20:43,480 --> 00:20:48,020
+ممكن ننجلّ؟ ما .. إذا أنا عندي طبقات مُتعددة
+
+262
+00:20:48,020 --> 00:20:54,720
+بيحاول الصبغة تخرج منها، لكن بتفشل لأنّ في طبقات
+
+263
+00:20:54,720 --> 00:21:00,470
+مُتعاقبة من الـ .. لكن لو كانت طبقة واحدة سهل تطلع
+
+264
+00:21:00,470 --> 00:21:04,910
+منها الـ Gram Negative على الجهة هذه، فاكرينها؟ لا
+
+265
+00:21:04,910 --> 00:21:07,930
+يرتبط لطبقة واحدة من الببتيدوجلايكان بينما الـ Gram
+
+266
+00:21:07,930 --> 00:21:12,130
+Positive يرتبط لـ Multiple Layer من اللي هو اللي
+
+267
+00:21:12,130 --> 00:21:15,870
+بده ببتيدوجلايكان، هذه نقطة أساسية في الفروقات بين
+
+268
+00:21:15,870 --> 00:21:20,630
+الاثنين، بس في كمان نقطة هنا في الـ Gram Negative
+
+269
+00:21:20,630 --> 00:21:23,630
+في كمية دهون عالية جدًا، طب إيش داخل الدهون في
+
+270
+00:21:23,630 --> 00:21:28,720
+الموضوع؟ الدهون موجودة على الـ cell wall من برّا فالـ
+
+271
+00:21:28,720 --> 00:21:33,300
+decolourizer اللي هو إيه؟ إيثانول، يزيل الدهون، بيذيبها
+
+272
+00:21:33,300 --> 00:21:37,540
+فلما يذيب الدهون بيصير فيه مسامات أوسع فإمكانية
+
+273
+00:21:37,540 --> 00:21:42,040
+خروج الصبغة كم بتكون إيه؟ أسهل على مقابل الـ Gram
+
+274
+00:21:42,040 --> 00:21:46,900
+positive، كمية الدهون نادرة جدًا وبالتالي لما بتدوب
+
+275
+00:21:46,900 --> 00:21:50,500
+مش هتطلق منها مسامات عالية إيه؟ دلوقتي فرق الأنصار
+
+276
+00:21:50,500 --> 00:21:54,270
+هنا؟ فرقين أساسيين، multiple layers of peptido
+
+277
+00:21:54,270 --> 00:21:58,370
+glycan على الجهة هذه وكمية دهون قليلة هنا على
+
+278
+00:21:58,370 --> 00:22:03,150
+الطرف هنا، one single layer of peptido glycan وفين
+
+279
+00:22:03,150 --> 00:22:11,230
+دي large amount of lipids
+
+280
+00:22:11,230 --> 00:22:15,030
+هذا الشكل يعني اللي قدامنا توضيحية يعني
+
+281
+00:22:15,030 --> 00:22:20,470
+توضيحية عن مدى الفروقات ما بين the Gram positive and
+
+282
+00:22:20,470 --> 00:22:23,390
+the cell wall envelope اللي هو cell wall يعني والـ
+
+283
+00:22:23,390 --> 00:22:27,230
+Gram negative cell wall، اتطلعوا هنا الببتيدوجلايكان
+
+284
+00:22:27,230 --> 00:22:30,650
+واضح أنه عبارة إيه؟ عن multiple layer بينما هنا
+
+285
+00:22:30,650 --> 00:22:35,730
+single layer، هنا في حيث ما outer membrane بس في
+
+286
+00:22:35,730 --> 00:22:39,570
+الجرام بوزيتيف ما فيش outer membrane، مين أحسن؟ الجرام
+
+287
+00:22:39,570 --> 00:22:43,810
+بوزيتيف ولا الجرام نيجاتيف؟ مين أكثر فعالية؟ الجرام
+
+288
+00:22:43,810 --> 00:22:50,930
+نيجاتيف ولا الجرام بوزيتيف؟ مش سامع أحد يرفع يده
+
+289
+00:22:50,930 --> 00:22:59,470
+ويقول جواب، عشان اللي بيقول جواب بيفسّر ليه، شكّوا أنفسكم
+
+290
+00:22:59,470 --> 00:23:03,170
+أنّ تنخدعوا في الـ microbiology بالمقارنات عندما
+
+291
+00:23:03,170 --> 00:23:07,910
+تسألوا مين أفضل بتكونوا تفكروا كويس، طالما أنه
+
+292
+00:23:07,910 --> 00:23:11,630
+اثنين موجودين يبقى هتلاقوا أنّ لكل واحد منهم فيه
+
+293
+00:23:11,630 --> 00:23:16,330
+مزايا وفيه عيوب، هذا الكلام بينطبق على كل شيء موجود
+
+294
+00:23:16,330 --> 00:23:17,290
+منه اثنين أو أكثر
+
+295
+00:23:20,440 --> 00:23:24,340
+المقارنات بتكون صعبة، مين أحسن؟ مين أحسن في وضع
+
+296
+00:23:24,340 --> 00:23:29,160
+معين؟ الـ Gram negative شفنا إحنا أنه فيها single
+
+297
+00:23:29,160 --> 00:23:36,140
+layer، الـ single layer هذا كافية ومعطي الوظائف
+
+298
+00:23:36,140 --> 00:23:40,440
+الأساسية للـ cell wall والخلية عايشة بأمان وتمام
+
+299
+00:23:40,440 --> 00:23:47,660
+بالعكس هي مقاومة للدموع بتاعتنا، هي مقاومة للبنسلين
+
+300
+00:23:47,660 --> 00:23:51,460
+اللي فاكرين أنه بيكسر الـ cross-link هي مقاومة للـ
+
+301
+00:23:51,460 --> 00:23:55,620
+vancomycin برضه اللي بيكسر الـ cross-link، يبقى مش
+
+302
+00:23:55,620 --> 00:23:58,660
+معناه الحاجة أنّ ما فيش فيها multiple layer تمام؟
+
+303
+00:23:58,660 --> 00:24:02,380
+معناه الحاجة أنّ هي الأضعف؟ لأ، مش الأضعف بالعكس
+
+304
+00:24:02,380 --> 00:24:06,200
+هنشوف كمان شوية مزايا للـ outer membrane اللي
+
+305
+00:24:06,200 --> 00:24:10,400
+أنتروا في الجرام بوزيتيف ونيجاتيف، لكن في
+
+306
+00:24:10,400 --> 00:24:16,500
+النهاية هدول عبارة عن تركيبين من خليتين مختلفتين وكل
+
+307
+00:24:16,500 --> 00:24:26,100
+واحد بيلو تراكيب أو خدنا نقول مزايا خاصة فيه، هذه
+
+308
+00:24:26,100 --> 00:24:28,880
+الصورة بوضح أنّ الـ lysozyme يوم بيقطع تكرير الـ
+
+309
+00:24:28,880 --> 00:24:32,780
+alternative units اللي هو الـ N والـ M اللي عفونا
+
+310
+00:24:32,780 --> 00:24:35,960
+الـ M والـ B بيقطع بينهم الـ lysozyme اللي هي الدموع
+
+311
+00:24:35,960 --> 00:24:40,670
+اللي موجودة فيه الدموع، destruction للخلايا وعشان
+
+312
+00:24:40,670 --> 00:24:45,070
+قلت لكوا أنّ الجرام بوزيتيف بتأثر أكثر من مين؟ من الـ
+
+313
+00:24:45,070 --> 00:24:48,090
+Gram Negative، لذلك معظم الالتهابات اللي عايبين بيهم
+
+314
+00:24:48,090 --> 00:24:52,630
+بسببها الـ Gram Negative، الجرام بوزيتيف بتموت من
+
+315
+00:24:52,630 --> 00:24:55,930
+الدموع صح؟ فاللي حاسس بالالتهاب مين؟ الـ Gram
+
+316
+00:24:55,930 --> 00:24:59,290
+-Negative
+
+317
+00:24:59,290 --> 00:25:05,370
+أحد التراكيب المهم جدًا في الجرام بوزيتيف بكتيريا
+
+318
+00:25:05,370 --> 00:25:09,820
+حارس أنها تايكويك اسيد، والـ Teichoic Acid زي ما مقول
+
+319
+00:25:09,820 --> 00:25:15,980
+في اللوحة ماخذ أو جاي من مكانين، واحد جاي من الـ
+
+320
+00:25:15,980 --> 00:25:19,100
+cell membrane من تحته، شايفينه؟ اللي دول تبعته؟
+
+321
+00:25:19,100 --> 00:25:23,700
+واحد جاي من الـ cell wall، اللي جاي من الـ cell wall
+
+322
+00:25:23,700 --> 00:25:29,240
+بنطلق عليه هكذا، Teichoic Acid، التاني اللي جاي من
+
+323
+00:25:29,240 --> 00:25:33,540
+الـ cell membrane بنطلق عليه Lipo-Teichoic Acid، Lipo
+
+324
+00:25:33,540 --> 00:25:36,840
+لأنه جاي من الـ Lipids تمام، Lipo-Teichoic Acid
+
+325
+00:25:37,020 --> 00:25:40,820
+والوظيفة أو وظائف مُتعددة، حندرسها لما نتكلم عن الـ
+
+326
+00:25:40,820 --> 00:25:47,420
+medical microbiogen chart، نيجي الآن للتركيب المهم
+
+327
+00:25:47,420 --> 00:25:51,080
+جدا في الـ Gram Negative bacteria اللي هو الـ the
+
+328
+00:25:51,080 --> 00:25:57,260
+outer membrane وهذه اللوحة اللي قدامنا تفاصيل في
+
+329
+00:25:57,260 --> 00:26:02,640
+حدّ فيكم بيتخيل القوام، القوام أقصد الـ consistency
+
+330
+00:26:02,640 --> 00:26:06,430
+تبع الـ cell membrane كيف القوام تبعه يعني؟ يعني
+
+331
+00:26:06,430 --> 00:26:11,710
+مثلًا صلب زي الورقة يعني اللي بنمزّقه ولا زي الجلد
+
+332
+00:26:11,710 --> 00:26:17,430
+تبعنا طري ولا إيش؟ كيف القوام تبعه؟ cell membrane
+
+333
+00:26:17,430 --> 00:26:24,410
+أوضّح لكوا كيف القوام تبعه، دي بصحن زيت زيتون، بحطّ
+
+334
+00:26:24,410 --> 00:26:27,830
+إصبعك في الصحن وامسكه هيك، إيش بيصير في زيت
+
+335
+00:26:27,830 --> 00:26:33,150
+زيتون؟ بنكسّره نصين، تمام؟ وبعد ما إحنا إصبعنا
+
+336
+00:26:33,150 --> 00:26:38,710
+يمر، إيش بيعمل؟ بيرجع تاني، عارفين القوام تبع الـ cell
+
+337
+00:26:38,710 --> 00:26:45,770
+membrane أقل شوية كمان من ذاته؟ بس لأنه صغير بيكون
+
+338
+00:26:45,770 --> 00:26:49,750
+متناسب نوعًا ما، لأنّ حجمه صغير بيكون متناسب، عارفين
+
+339
+00:26:49,750 --> 00:26:53,210
+إيش القوام هذا مهم؟ شفت الحركة تبع الإصبع هنا؟
+
+340
+00:26:53,210 --> 00:26:58,370
+تخيلوا أنه فيه هنا هاي الـ cell membrane وهنا فيه
+
+341
+00:26:58,370 --> 00:27:03,270
+channel قناة لاستقبال غذاء معيّن، طب الغذاء إيه عشان
+
+342
+00:27:03,270 --> 00:27:10,320
+تتنمّى الخلية؟ لو صلب هذا ما يتحركش، لكن هذا ممكن ينتقل
+
+343
+00:27:10,320 --> 00:27:16,360
+من مكانه إلى مكان آخر، تشبيه لذيذ وجيّد بيقول أنّ الـ
+
+344
+00:27:16,360 --> 00:27:21,020
+cell membrane هو عبارة عن بروتينات تسبح في بحر من
+
+345
+00:27:21,020 --> 00:27:25,320
+الدهون، تسبح يعني بمعنى آخر إيش السباحة؟ بيقدر ينتقل
+
+346
+00:27:25,320 --> 00:27:31,120
+من مكان إلى مكان، فهو عبارة عن يعني قوام لين تمام و
+
+347
+00:27:31,120 --> 00:27:33,940
+تنتقل فيه البروتينات بحرية نوعًا ما
+
+348
+00:27:38,150 --> 00:27:43,550
+أنا بدرس ماستر ميكروبيولوجي وبدرسهم صحّ هذا
+
+349
+00:27:43,550 --> 00:27:46,210
+الموضوع اللي هو الـ .. الـ .. الـ .. الـ outer
+
+350
+00:27:46,210 --> 00:27:48,290
+membrane of the Gram negative في الـ clinical
+
+351
+00:27:48,290 --> 00:27:52,590
+microbiology وبياخد الموضوع لحاله لحاله، إمّا محاضرة
+
+352
+00:27:52,590 --> 00:27:56,330
+كاملة تلت ساعات أو محاضرة تانية ست ساعات، بس حين
+
+353
+00:27:56,330 --> 00:27:59,290
+نتكلم عن الجزئية هذه اللي هي اللي قدامنا، لكن إحنا
+
+354
+00:27:59,290 --> 00:28:02,970
+اليوم بدنا نأخذ فكرة عامة، أنا بقول .. قلت
+
+355
+00:28:02,970 --> 00:28:08,560
+المعلومة السابقة لأدلّل على أهمية هذا التركيب، مش
+
+356
+00:28:08,560 --> 00:28:12,620
+بدنا نعقّد الأمر لأنّنا نبسّطه اليوم، لكن هو مهم ومهم
+
+357
+00:28:12,620 --> 00:28:17,160
+جدا إلينا وده البكتيريا إلينا من ناحية أنّ إحنا ممكن
+
+358
+00:28:17,160 --> 00:28:21,620
+نتعرّف على البكتيريا من خلاله، وده البكتيريا هو أحد
+
+359
+00:28:21,620 --> 00:28:26,440
+أهم عوامل الأمراض في الـ Gram Negative بكتيريا وأحد
+
+360
+00:28:26,440 --> 00:28:30,540
+أهم عوامل الخداع في الـ Gram Negative بكتيريا، كيف
+
+361
+00:28:30,540 --> 00:28:36,560
+أهم عوامل الخداع؟ شايفين الجزء هذا؟ لفوق
+
+362
+00:28:38,090 --> 00:28:42,450
+هذا بيسمّوه O-side
+
+363
+00:28:42,450 --> 00:28:53,810
+-chain أو بيسمّوه O-antigen، بس
+
+364
+00:28:53,810 --> 00:28:57,690
+بتعرفوش إيه الـ antigen وبتعرفوش، Antigen هو الجسم
+
+365
+00:28:57,690 --> 00:29:00,770
+الغريب اللي يدخل جسم الإنسان أو الحيوان، الجسم
+
+366
+00:29:00,770 --> 00:29:03,650
+الإنسان أو الحيوان بيكوّن له أجسام مضادة وتتفاعل من أي شكل
+
+367
+00:29:03,650 --> 00:29:07,250
+أجسام مضادّة أو تتفاعل specifically مع هذا الـ
+
+368
+00:29:07,250 --> 00:29:12,470
+antigen، الآن بالنظرية الانتيجينات الفيروسات الـ
+
+369
+00:29:12,470 --> 00:29:16,930
+influenza أو الرشح المفروض لما تصيبنا مرّة جسمنا
+
+370
+00:29:16,930 --> 00:29:21,430
+يكون جسمنا مُكوّن مضادّة، المرة الجاية ممنوع يرجع مرّة تانية
+
+371
+00:29:21,430 --> 00:29:24,030
+ولكن واقع الأمر أنّ إحنا بنمرض في الـ influenza
+
+372
+00:29:24,030 --> 00:29:30,150
+عشرات المرّات تمام، خاصّة موقعة الامتحانات مرارًا
+
+373
+00:29:30,150 --> 00:29:34,550
+متكررًا، لأنه بيصير في عندي ما يُسمّى بالـ antigenic
+
+374
+00:29:34,550 --> 00:29:39,100
+variation، التغيّرات الانتيجينية، إيش هي التغيّرة
+
+375
+00:29:39,100 --> 00:29:44,160
+الانتيجينية؟ يعني بيصير طفرة فبتغيّر تركيب الانتيجين 
+
+376
+00:29:44,160 --> 00:29:48,660
+إذا تغير تركيب الانتيجين جهاز المناعة زي بصمة
+
+377
+00:29:48,660 --> 00:29:54,080
+الكمبيوتر تمام؟ لا يستطيع أن يتعرف على الميكروب من 
+
+378
+00:29:54,080 --> 00:29:56,960
+شكله وإنما يتعرف عليه من خلال مين؟ الانتيجين
+
+379
+00:29:56,960 --> 00:30:00,600
+وبالتالي إذا تغير الانتيجين لن يتعرف عليه، هيتعرف 
+
+380
+00:30:00,600 --> 00:30:04,440
+عليه مرة كأنه مخلوق جديد هيكوّن له أجسام مضادة مرة،
+
+381
+00:30:04,440 --> 00:30:08,840
+أخرى، يعني هياخد الـ course مرة ثانية، لو تلكوا أن
+
+382
+00:30:08,840 --> 00:30:12,720
+هذا الجزر اللي هو اسمه Antigen أنه في الـ Gram
+
+383
+00:30:12,720 --> 00:30:17,340
+Negative Bacteria هو المسؤول عن الـ Antigenic
+
+384
+00:30:17,340 --> 00:30:21,080
+Variation التغيير الانتجيني أو التنوع الانتجيني
+
+385
+00:30:21,080 --> 00:30:26,640
+للـ Gram Negative Bacteria بس غير السكر هذا أو هذا
+
+386
+00:30:26,640 --> 00:30:31,320
+أو هذا أو .. أيوة ترتيبه غيري إما ترتيب أو سكر 
+
+387
+00:30:31,320 --> 00:30:36,900
+اتغير بسبب الانتيجين الجديد، وليؤدي على ذلك تسمعوا
+
+388
+00:30:36,900 --> 00:30:40,120
+بالـ E. coli ولا ما سمعتوش فيها؟ طيب ده اللي ما سمعش
+
+389
+00:30:40,120 --> 00:30:45,620
+في الـ E. coli  الاسم فيها وبعد ذلك هتصير أشهر
+
+390
+00:30:45,620 --> 00:30:49,980
+اسم على الإطلاق لديكم في الـ microbiology  E. coli أو
+
+391
+00:30:49,980 --> 00:30:53,900
+E. coli وهي بكتيريا موجودة فيه بشكل طبيعي بأمعاء
+
+392
+00:30:53,900 --> 00:30:59,580
+الإنسان، أمهات؟ ويعني من أكثر الميكروبات استئناسًا
+
+393
+00:30:59,580 --> 00:31:02,600
+البشر، بيتسلسلوا فيها بيفحصوها وبيشتغلوا عليها
+
+394
+00:31:02,600 --> 00:31:07,410
+دراساتهم العلمية، الـ E. coli المفروض أن الأمر ده
+
+395
+00:31:07,410 --> 00:31:11,570
+بصورة طبيعية في أمعائنا، لكن بعض أصناف منها بسبب
+
+396
+00:31:11,570 --> 00:31:15,490
+الإسهال، فلازم نتميز بين الطبيعية وبين السبب لمين ده
+
+397
+00:31:15,490 --> 00:31:20,050
+الإسهال، فميزناهم عن طريق الانتيجينات اسمها E. coli
+
+398
+00:31:20,050 --> 00:31:24,170
+O157H6
+
+399
+00:31:24,170 --> 00:31:28,290
+بقى E. coli لحالها، سنة أخرى دخلنا في تفاصيل
+
+400
+00:31:28,290 --> 00:31:33,630
+الانتيجينات تبعها، بدينا نتكلم عن O-antigen وعن H،
+
+401
+00:31:33,630 --> 00:31:36,230
+يعني اللي احنا عارفينه الـ O عارفناه لما نقولها هو
+
+402
+00:31:36,230 --> 00:31:39,270
+هذا الجزء من مين؟ من الـ outer، من الـ LPS، اللي 
+
+403
+00:31:39,270 --> 00:31:41,310
+مقولته في الـ cell wall، اللي مقولته في الجرام،
+
+404
+00:31:41,310 --> 00:31:46,950
+negative، and back to you، لأن ماذا تالي O157؟ أول
+
+405
+00:31:46,950 --> 00:31:52,270
+أنتيجين، أول أنتجين سميناه O1، اتغير تغيير طفيف، مش
+
+406
+00:31:52,270 --> 00:31:58,250
+حاجة أسميه أنا B، هسميه O2، لأن أنا كلمة واحد
+
+407
+00:31:58,250 --> 00:32:04,420
+ثاني، نوع ثالث، O3 ضلنا نعد و نسجل لما وصلنا لمين؟
+
+408
+00:32:04,420 --> 00:32:09,000
+طبعًا في أرقام أعلى من ذلك الآن، هذا بنتكلم عن مين؟
+
+409
+00:32:09,000 --> 00:32:15,800
+عن E. coli واحدة، صنف واحد، استطاعت أن تكتسب
+
+410
+00:32:15,800 --> 00:32:22,080
+أنتيجينات أو تنوع أنتجيني، خلّى منها مئات السلالات،
+
+411
+00:32:22,080 --> 00:32:28,720
+مئات السلالات فقط عشان أنها مين؟ أو side-chain أو
+
+412
+00:32:28,720 --> 00:32:34,090
+أو أنتجين، الـ O-antigen له أهمية للبكتيريا بأنه
+
+413
+00:32:34,090 --> 00:32:37,030
+أعطاه قدرة على الـ Antigenic Variation، طب مش 
+
+414
+00:32:37,030 --> 00:32:40,450
+أهميته بالنسبة لي أنا كمختبر كـ Microbiologist
+
+415
+00:32:40,450 --> 00:32:45,930
+أهميته إنه أنا تعرفت على البكتيريا من خلالها،
+
+416
+00:32:45,930 --> 00:32:49,610
+بمعنى أنا عزلت بكتيريا بدي أعرف هل هي O157 ولا مش
+
+417
+00:32:49,610 --> 00:32:53,650
+O157 بجيب أجسام مضادة antibodies وبحطها على
+
+418
+00:32:53,650 --> 00:32:57,770
+البكتيريا، اللي اتفعلت معاها نعم هي O157، ما اتفعلت
+
+419
+00:32:57,770 --> 00:33:02,610
+معاها مش O157، يبقى أنا استخدمت الآن معرفة بالـ O
+
+420
+00:33:02,610 --> 00:33:06,750
+-antigen في التعرف على البكتيريا، ويُوفر طول
+
+421
+00:33:06,750 --> 00:33:14,590
+النهاردة معرفة جيدة، طيب
+
+422
+00:33:14,590 --> 00:33:17,180
+احنا بنتكلم.. قاعدين أنا عامل.. عن الـ Outer
+
+423
+00:33:17,180 --> 00:33:22,240
+Membrane of Gram Negative Bacteria مكون من
+
+424
+00:33:22,240 --> 00:33:28,840
+Lipopolysaccharide LPS الـ Outer Membrane مكون من
+
+425
+00:33:28,840 --> 00:33:32,300
+مين بيقولنا؟ من Lipopolysaccharide هنقولكوا من وين
+
+426
+00:33:32,300 --> 00:33:35,520
+جاي الـ Lipopolysaccharide طلع ليه هنا، هاي الـ cell
+
+427
+00:33:35,520 --> 00:33:43,240
+wall، هاي الـ lipid، فيه جزء اسمه core، يا
+
+428
+00:33:43,240 --> 00:33:49,850
+ده اسم الـ lipid إيه؟ وهذا core polysaccharide
+
+429
+00:33:49,850 --> 00:33:56,970
+وهذا اسمه O side chain اللي حكينا عنه قبل، هو الـ O
+
+430
+00:33:56,970 --> 00:34:00,850
+أنتيجين، نفس الاسم اللي بقانونيه على الصحيح شغل ثاني
+
+431
+00:34:00,850 --> 00:34:04,710
+في هذا التركيب اللي هو الـ O side chain واللي
+
+432
+00:34:04,710 --> 00:34:08,750
+بداية الـ core تقريبًا متشابه فيهم كلهم وبالتالي هو
+
+433
+00:34:08,750 --> 00:34:14,410
+مجرد مسافة تفصل لبث A عن مين؟ عن الـ O-sided chain
+
+434
+00:34:14,410 --> 00:34:18,870
+مسافة التي طبعًا لها أهميتها لكن ليس في هذا المؤشر
+
+435
+00:34:18,870 --> 00:34:24,390
+اللي احنا بنتكلم فيه، اللبث A اللي هو اسم ثاني يا
+
+436
+00:34:24,390 --> 00:34:28,130
+بنت لازم نعرفه الآن ونحفظه كويس اسمه endotoxin
+
+437
+00:34:28,130 --> 00:34:34,130
+اندو؟
+
+438
+00:34:34,130 --> 00:34:39,830
+إيش يعني اندو؟ يعني سم داخلي، إيش يعني سم داخلي؟
+
+439
+00:34:39,830 --> 00:34:44,990
+يعني هو تصنيف لهذا السم على أنه هو جزء لا يتجزأ من
+
+440
+00:34:44,990 --> 00:34:48,850
+تكوين الخلية، integral part أقدر أسألك، لا يتجزأ
+
+441
+00:34:48,850 --> 00:34:53,010
+من الخلية، endotoxin، لأن ما أتوقع الخلية هي حية
+
+442
+00:34:53,010 --> 00:34:56,630
+ما تطلق هذا الـ toxin، لأنه جزء من تكوينها، حد
+
+443
+00:34:56,630 --> 00:35:01,070
+برمي، بمسك إيده وبقطعها وما فيها سلاح، لأ، لكن
+
+444
+00:35:01,070 --> 00:35:06,650
+لو موت إيده تتحلل وكل جسمه يتحلل، عندما يتحلل
+
+445
+00:35:06,650 --> 00:35:11,610
+الاسم ينطلق من الـ endotoxin، إذا البكتيريا هي حية
+
+446
+00:35:11,610 --> 00:35:16,670
+ما بتنترش endotoxin، ماذا بتنتجه؟ وهي ميتة لما تبدأ
+
+447
+00:35:16,670 --> 00:35:22,490
+التحلل، وأعطيكم نموذج خطير جدا على هذا أو على هذه
+
+448
+00:35:22,490 --> 00:35:29,410
+الحقيقة، مريض تم تشخيصه على أنه عنده septicemia
+
+449
+00:35:29,410 --> 00:35:31,610
+septicemia يعني البكتيريا موجودة في الدم، موجودة في
+
+450
+00:35:31,610 --> 00:35:35,820
+أين؟ في الدم، عملنا له مزرعة واتعرفنا على البكتيريا
+
+451
+00:35:35,820 --> 00:35:39,440
+وطلع اسمها Escherichia coli وعملنا لها فحص حساسية
+
+452
+00:35:39,440 --> 00:35:42,260
+للموضوع الحيوي، لجيناها sensitive لجيناها gentamicin
+
+453
+00:35:42,260 --> 00:35:48,580
+فالطبيب قرر يعطي المريض gentamicin، البكتيريا ماتت
+
+454
+00:35:48,580 --> 00:35:54,480
+والمريض مات، مات المريض بموت البكتيريا، البكتيريا
+
+455
+00:35:54,480 --> 00:36:00,730
+ماتت فجأة أطلقت كميات ضخمة من الـ endotoxin، صار عنده
+
+456
+00:36:00,730 --> 00:36:05,350
+ما يسمى بالـ Gram Negative Septic Shock، Shock يعني
+
+457
+00:36:05,350 --> 00:36:10,310
+صدمة، وبتقدر لوقفها لأن الـ endotoxin له تأثيرات منها
+
+458
+00:36:10,310 --> 00:36:14,650
+بنزل الـ blood pressure، بيعمل vasodilatation في
+
+459
+00:36:14,650 --> 00:36:21,270
+الآخر بيعمل shock، إذن الـ endotoxin أحد أهم عوامل
+
+460
+00:36:21,270 --> 00:36:26,120
+الخطورة في المرضى اللي عندهم Gram Negative Bacteria، هذا كلام
+
+461
+00:36:26,120 --> 00:36:32,060
+غير موجود في الـ Gram Positive، فقط في الـ Gram Negative، فجأة
+
+462
+00:36:32,060 --> 00:36:36,720
+أول معلومة عرفتوها أنتو Negative، نعم منها إن كل
+
+463
+00:36:36,720 --> 00:36:40,820
+الـ Gram Negative تحتوي على endotoxin بينما الـ Gram
+
+464
+00:36:40,820 --> 00:36:46,320
+Positive لا تحتوي على endotoxin، إيش السبب إنّها تحتوي
+
+465
+00:36:46,320 --> 00:36:48,900
+على endotoxin، إن هو عندها Outer membrane، الأوتر
+
+466
+00:36:48,900 --> 00:36:52,000
+membrane اللي متكونة من Lipopolysaccharide، الليبو
+
+467
+00:36:52,000 --> 00:36:54,760
+بوليساكرايد، ده بيعني إيه؟ Lipid A و core
+
+468
+00:36:54,760 --> 00:36:57,400
+polysaccharide و O side chain، الـ O side chain
+
+469
+00:36:57,400 --> 00:37:02,900
+فهمتوها، هي الـ O antigen اللي من خلاله جسمنا بيتعرف
+
+470
+00:37:02,900 --> 00:37:11,420
+على البكتيريا وبيحاول يحاربها، بيحاول يحاربها 
+
+471
+00:37:11,420 --> 00:37:17,240
+هاي
+
+472
+00:37:17,240 --> 00:37:19,640
+التراكيب اللي ذكرتوها بيه قبل شوية، الـ Lipid
+
+473
+00:37:19,640 --> 00:37:22,860
+polysaccharide اللي هو الـ LPS، خاصة اتصال اللي نسميه
+
+474
+00:37:22,860 --> 00:37:27,100
+LPS، اتصال اللي نسميه LPS، قدر ما كنا لبد إيه، والـ
+
+475
+00:37:27,100 --> 00:37:30,460
+core polysaccharide اللي هو الـ O أنتجين، ولاحظوا
+
+476
+00:37:30,460 --> 00:37:33,540
+هنا جملة مكتوبة هنا highly variable، اللي يعني
+
+477
+00:37:33,540 --> 00:37:38,460
+متغير بشكل كبير مما يعطي فرصة أكبر، وهذا الأمر صار
+
+478
+00:37:38,460 --> 00:37:41,980
+لنا مقضة على أنه نفسر، لماذا نقصد بنفس البكتيريا
+
+479
+00:37:41,980 --> 00:37:45,880
+مرارا وتكرارًا، واقع الأمر هي مش نفس البكتيريا
+
+480
+00:37:45,880 --> 00:37:49,660
+وإنما فيها شوية modification متعلقة بالانتيجين
+
+481
+00:37:52,500 --> 00:37:55,680
+نجيب على الـ functions of the cell wall، ما هي 
+
+482
+00:37:55,680 --> 00:38:02,300
+وظائف الـ cell wall اللي ممكن احنا نلخصها في شكل
+
+483
+00:38:02,300 --> 00:38:05,500
+نقاط، رقم واحد maintaining the cell's
+
+484
+00:38:05,500 --> 00:38:09,500
+characteristic shape، هي المسئولة عن شكل الخلية
+
+485
+00:38:09,500 --> 00:38:12,700
+الأول، أول يعني إيه ده campus ولا coccus هي
+
+486
+00:38:12,700 --> 00:38:17,840
+المسئولة لأنها rigid، عشان rigid صلبة، وزي ما
+
+487
+00:38:17,840 --> 00:38:21,220
+قلتلكوا النباتات بتاخد شكل محدد ولا تتحرك لأنها
+
+488
+00:38:21,220 --> 00:38:26,810
+cell wall، طيب countering the effect of osmotic
+
+489
+00:38:26,810 --> 00:38:34,870
+pressures، تخيلوا معايا بكتيريا أخدنا بكتيريا تمام
+
+490
+00:38:34,870 --> 00:38:38,150
+وحطيناها في السيفون وعملنا لها flushing لقى في
+
+491
+00:38:38,150 --> 00:38:42,990
+السيفون flushing، البكتيريا كانت في المختبر مزروعة
+
+492
+00:38:42,990 --> 00:38:46,250
+في media مبسوطة من كيفها، ودروفها ممتازة رائعة جدا
+
+493
+00:38:46,250 --> 00:38:51,470
+والـ osmotic pressure ملائم جدا لها، لما حطيناها في
+
+494
+00:38:51,470 --> 00:38:56,710
+الميه، الميه مش فيها ملح، صار hypo.. مش hypo..
+
+495
+00:38:56,710 --> 00:39:00,390
+مش iso.. صار hypo وبالتالي الآن ضغط الـ Osmosis
+
+496
+00:39:00,390 --> 00:39:04,050
+اللي في الميه هيسحب الأملاح من البكتيريا ويموتها، والـ
+
+497
+00:39:04,050 --> 00:39:09,930
+ميه تدخل إلى البكتيريا تنفخها، فالآن مين المسؤول
+
+498
+00:39:09,930 --> 00:39:12,510
+عن حماية البكتيريا في هذه اللحظة الخطيرة في 
+
+499
+00:39:12,510 --> 00:39:16,870
+حياتها؟ الـ cell wall، طيب مع إنّ السيفون ضربناه راحت
+
+500
+00:39:16,870 --> 00:39:20,790
+على المجاري، أكرركم الله فيه toxin وفيه osmotic
+
+501
+00:39:20,790 --> 00:39:23,430
+pressures مختلفة في الآخر راحت على البحرانا بتبقى
+
+502
+00:39:23,430 --> 00:39:27,910
+البحر مظبوط في البحر كمية الملح عالية جدا عكس الماء
+
+503
+00:39:27,910 --> 00:39:31,570
+اللي حلو اللي كنا نتكلم عنها يبقى المسؤول عن حماية
+
+504
+00:39:31,570 --> 00:39:36,410
+البكتيريا من الـ variable osmotic pressures هو
+
+505
+00:39:36,410 --> 00:39:39,970
+الـ cells providing attachment site for
+
+506
+00:39:39,970 --> 00:39:42,690
+bacteriophages بتسمعوا إن في فيروسات بتصيب
+
+507
+00:39:42,690 --> 00:39:46,910
+البكتيريا الفيروسات اللي بتصيب البكتيريا بيسموها
+
+508
+00:39:46,910 --> 00:39:51,830
+bacteriophage أو phages يعني باختصار و بالعربي لا
+
+509
+00:39:51,830 --> 00:39:55,920
+آكلات البكتيريا تسمية خاطئة، لكن شاعت وما زالت هي
+
+510
+00:39:55,920 --> 00:39:59,280
+نفس التسمية، لأن الـ phage بيكون أكبر، هل يبقى بيكون
+
+511
+00:39:59,280 --> 00:40:04,460
+أصغر وبتتغذى على البكتيريا؟ من جميعها، it provides
+
+512
+00:40:04,460 --> 00:40:09,680
+a surface to attach، فاكرين الفجلة؟ من انطلاقتها
+
+513
+00:40:10,930 --> 00:40:13,110
+تخيلوا لو الـ Cell Membrane .. لو الـ Cell 
+
+514
+00:40:13,110 --> 00:40:15,710
+Membrane هو الفجر من الفجر .. الفجر كل ما تحرك ..
+
+515
+00:40:15,710 --> 00:40:19,170
+كل ما تحرك .. هتهز الـ Cell Membrane وهتفسر ..
+
+516
+00:40:19,170 --> 00:40:24,250
+هتكسر لكن هذه بتوفر وأنها rigid platform للفجر ..
+
+517
+00:40:24,250 --> 00:40:29,030
+لإيجاد الفمري، أنقلا، Play an essential role in cell
+
+518
+00:40:29,030 --> 00:40:32,610
+division مسئولة أو لها دور أساسي في عملية
+
+519
+00:40:32,610 --> 00:40:36,360
+الانقسام، ده بقى نخليه be the site of major
+
+520
+00:40:36,360 --> 00:40:38,980
+antigenic determinants of the cell surface مش هي
+
+521
+00:40:38,980 --> 00:40:42,840
+.. احنا قولنا هي الجزء الخارجي للخلية أو هي الفاصل
+
+522
+00:40:42,840 --> 00:40:46,240
+ما بين الداخل والخارج وبالتالي عليها بيكون اللي هو
+
+523
+00:40:46,240 --> 00:40:49,800
+الـ antigens اللي من خلالها جسمنا بتعرف على
+
+524
+00:40:49,800 --> 00:40:55,480
+البكتيريا طيب هناك بكتيريا wall-less wall-less
+
+525
+00:40:55,480 --> 00:41:00,960
+يعني منهاش جدار اسم والاسم هذا .. أنا بكتبه بخط
+
+526
+00:41:09,340 --> 00:41:15,960
+mycoplasma الـ mycoplasma تحفظ هذه هي الوحيدة من
+
+527
+00:41:15,960 --> 00:41:20,720
+البكتيريا اللي تستطيع المعيشة بدون cell wall بدون
+
+528
+00:41:20,720 --> 00:41:25,380
+cell wall تنقسم و تتكاثر و بتعيش في البيئة بدون
+
+529
+00:41:25,380 --> 00:41:30,440
+cell wall اسمها mycoplasma وهي أصغر بمناسبة هي
+
+530
+00:41:30,440 --> 00:41:36,580
+أصغر بكتيريا اسمها أصغر بكتيريا اسمها كم مرة
+
+531
+00:41:36,580 --> 00:41:41,150
+mycoplasma طب طلعوا معايا الـ .. الموضوع من زاوية
+
+532
+00:41:41,150 --> 00:41:47,130
+مختلفة بكتيريا أصلا كلها cell wall حطناها في
+
+533
+00:41:47,130 --> 00:41:50,230
+saline تلاعوا الـ saline؟ الـ normal saline محلول
+
+534
+00:41:50,230 --> 00:41:54,630
+ملح محلول ملحي اللي ضغطه الأسموزي تبقى بوازي ضغطه
+
+535
+00:41:54,630 --> 00:41:57,850
+الأسموزي تبقى على الخلايا وحطينا بعد الـ .. الـ
+
+536
+00:41:57,850 --> 00:42:03,210
+saline بالبنسلين البنسلين هيدمر الـ cell wall تمام؟
+
+537
+00:42:03,210 --> 00:42:06,090
+لكن الخلية مش هتنفجر، عارفين ليه مش هتنفجر؟ إن
+
+538
+00:42:06,090 --> 00:42:10,050
+أنا موجودة في normal saline فقدت الـ cell wall لكن
+
+539
+00:42:10,050 --> 00:42:14,290
+تقتل اسموزيا الموتوسياب هو بالتالي طالب إيه؟ خلية
+
+540
+00:42:14,290 --> 00:42:17,790
+يمكن إنتاج هذا الكلام ولا مش ممكن إنتاجه؟ ممكن
+
+541
+00:42:17,790 --> 00:42:21,550
+إنتاجه واتسمى الخلية بدون cell wall بنسميها
+
+542
+00:42:21,550 --> 00:42:25,830
+protoplast واحنا واحنا كنا طلاب كنا نعمل
+
+543
+00:42:25,830 --> 00:42:30,970
+protoplast عشان ندمج خليتين مع بعض يعني خلية فيها
+
+544
+00:42:30,970 --> 00:42:35,870
+خصائص معينة وخلية أخرى فيها خصائص فنشيل نجشر الـ cell
+
+545
+00:42:35,870 --> 00:42:43,090
+wall ونحطهم مع بعض ونحط مادة لحمة تدمجهم مع بعض و
+
+546
+00:42:43,090 --> 00:42:45,830
+بالتالي نحصل على ما يسمى بـ protoplast fusion عشان
+
+547
+00:42:45,830 --> 00:42:50,310
+نحصل على خلية لها خصائص جديدة، لذلك يمكن للبكتيريا
+
+548
+00:42:50,310 --> 00:42:56,050
+اللي توفرتلها ظروف فيسيولوجية أن تعيش من غيرها من
+
+549
+00:42:56,050 --> 00:43:00,850
+غير cell wall بإضافة إما lysosomes زي اللي قلنا
+
+550
+00:43:00,850 --> 00:43:03,990
+عنه قبل شوية دموع عادي أو بعض الـ antibiotics زي
+
+551
+00:43:03,990 --> 00:43:09,410
+اللي هو الـ penicillin لكن خد بالك، لو خرجت إلى
+
+552
+00:43:09,410 --> 00:43:12,910
+البيئة، هتموت، هتموت لإنه بعد ذلك المقضر الأعلى
+
+553
+00:43:12,910 --> 00:43:18,550
+يتحمل اللي هو الضغط الأسموزي المتغيرة كمان مراجعين
+
+554
+00:43:18,550 --> 00:43:25,830
+بيستخدموه إما enzymes أو antibiotics هذه
+
+555
+00:43:25,830 --> 00:43:31,030
+البكتيريا ممكن نلاقي منها natural و بيسموها الـ
+
+556
+00:43:31,030 --> 00:43:36,450
+forms الـ forms أطلق على الصحيح يعني أشكال L يعني
+
+557
+00:43:36,450 --> 00:43:41,310
+أشكال L مش .. مش معنى الحاجة إنه هي حملة حرف L لأ
+
+558
+00:43:41,310 --> 00:43:46,530
+في حالة اسمها Lister Institute مؤسسة Lister و أنا
+
+559
+00:43:46,530 --> 00:43:51,570
+في بريطانيا طبعا هذه المؤسسة الـ .. الـ .. العلماء
+
+560
+00:43:51,570 --> 00:43:55,910
+اللي اكتشفوا هذه البكتيريا سموا الأشكال اللي
+
+561
+00:43:55,910 --> 00:43:58,970
+شافوها اللي بدون .. بدون cell wall على اسم المؤسسة
+
+562
+00:43:58,970 --> 00:44:05,570
+مثلا L forms طبعا الـ L forms هذه كمان مرة أما
+
+563
+00:44:05,570 --> 00:44:08,830
+بتكون Natural أو Artificial Artificial يعني احنا
+
+564
+00:44:08,830 --> 00:44:12,370
+نصنعها، احنا بنصنعها و بنصنعها لغرض زي ما قلتلكم
+
+565
+00:44:12,370 --> 00:44:20,190
+بنعمل Protoplast Fusion طيب
+
+566
+00:44:20,190 --> 00:44:23,450
+الـ cell membrane الأنواع الموجودة هنا كونها الـ
+
+567
+00:44:23,450 --> 00:44:28,090
+cell membrane بره ولا جوه؟ جوه على طول بعد الـ cell
+
+568
+00:44:28,090 --> 00:44:31,030
+wall إلى الداخل يكون احنا الدهان صح؟ يعني الملاصق
+
+569
+00:44:31,030 --> 00:44:36,460
+لمين؟ لـ الـ cell wall بيكون الـ cell membrane طبعا الـ
+
+570
+00:44:36,460 --> 00:44:40,860
+cell membrane من أعظم التراكيب اللي موجودة كنتوا
+
+571
+00:44:40,860 --> 00:44:45,500
+تعرفوا إن الـ cell wall صحيح له وظائف و راية لكن الـ
+
+572
+00:44:45,500 --> 00:44:48,660
+cell membrane ليها قل عنه تذكروا إن البكتيريا
+
+573
+00:44:48,660 --> 00:44:52,580
+مافيهاش mitochondria صح؟ مافيهاش عضيات أصلا
+
+574
+00:44:52,580 --> 00:44:56,360
+مافيهاش organelles اللي بيقوم بوظيفة الـ cell
+
+575
+00:44:56,360 --> 00:44:59,160
+membrane عفوا لإن الـ mitochondria هو الـ cell
+
+576
+00:44:59,160 --> 00:45:03,370
+membrane بيعمل عملية الـ oxygen phosphorylation و
+
+577
+00:45:03,370 --> 00:45:08,850
+معظم الـ energy requiring functions بتتم في الـ cell
+
+578
+00:45:08,850 --> 00:45:13,270
+membrane ومن ضمن الـ cell membrane في تركيب آخر
+
+579
+00:45:13,270 --> 00:45:19,190
+بنضيفه احنا اللي اسم خاص بأن هو لازم مني من الـ
+
+580
+00:45:19,190 --> 00:45:23,770
+cell membrane هي مثلا البكتيريا هي الـ cell
+
+581
+00:45:23,770 --> 00:45:27,470
+membrane بنلاقي فجأة الـ cell membrane صارت تتثنى
+
+582
+00:45:27,470 --> 00:45:34,080
+بهذا الشكل أو بهذا الشكل اللحظة هذه الـ .. الـ .. الـ ..
+
+583
+00:45:34,080 --> 00:45:34,580
+الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. ال
+
+584
+00:45:34,580 --> 00:45:34,720
+.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. ال
+
+585
+00:45:34,720 --> 00:45:35,360
+الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. ال
+
+586
+00:45:35,360 --> 00:45:36,440
+.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. ال
+
+587
+00:45:36,440 --> 00:45:40,900
+الـ .. الـ .. الـ .. الـ .. الـ ..
+
+588
+00:45:40,900 --> 00:45:44,340
+الـ ..
+
+589
+00:45:44,340 --> 00:45:45,220
+الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. ال
+
+590
+00:45:45,220 --> 00:45:45,680
+.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. ال
+
+591
+00:45:45,680 --> 00:45:45,720
+الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. ال
+
+592
+00:45:45,720 --> 00:45:45,780
+.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. ال
+
+593
+00:45:45,780 --> 00:45:54,000
+.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ ..
+
+594
+00:45:54,000 --> 00:45:55,060
+ال
+
+595
+00:46:02,020 --> 00:46:07,620
+والتنيات هذه سطحها مستغل وصار عنده كمان إمكانية
+
+596
+00:46:07,620 --> 00:46:14,620
+storage تخزينه فهذا الـ structure بيسموه هذا أو هذا
+
+597
+00:46:14,620 --> 00:46:21,260
+بيسموه mesosomes وبنجح
+
+598
+00:46:21,260 --> 00:46:24,720
+في الان ده لحد إن شاء الله نكمل نفس المحاضرة بنفس
+
+599
+00:46:24,720 --> 00:46:27,640
+الأرواح المرة القادمة إن شاء الله الـ mesosomes هي
+
+600
+00:46:27,640 --> 00:46:30,180
+النقطة اللي انتهينا عندها إن شاء الله
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/3_y6SgyBxoo_raw.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/3_y6SgyBxoo_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..490cef9724b8493d843bf402a286ab016624c419
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/3_y6SgyBxoo_raw.srt
@@ -0,0 +1,2412 @@
+1
+00:00:20,940 --> 00:00:23,200
+بسم الله الرحمن الرحيم احنا كنا بنقول انه ال ..
+
+2
+00:00:23,200 --> 00:00:26,620
+العنوان المحاضرة هو عبارة عن bacterial morphology
+
+3
+00:00:26,620 --> 00:00:30,960
+and cell structure و تحدثنا عن انه ال bacteriology
+
+4
+00:00:30,960 --> 00:00:34,320
+او microbiology كانوا يستخدموهم ك synonymous يعني
+
+5
+00:00:34,320 --> 00:00:36,600
+و كأنه هذا المعنى بيدل على هذا المعنى، معانهم
+
+6
+00:00:36,600 --> 00:00:39,720
+مختلفين ال bacteriology هو جزء من ال microbiology
+
+7
+00:00:40,160 --> 00:00:44,760
+ولكن كون هو الدُزق الأكبر فكان يعرف الكل بالدُزق
+
+8
+00:00:44,760 --> 00:00:49,960
+الأكبر تمام؟ فكان يعرف بهذا الشكل وبتالي هتلاقوا
+
+9
+00:00:49,960 --> 00:00:52,820
+أن معظم كتب الميكروبولية بتركز على ال bacterial
+
+10
+00:00:52,820 --> 00:00:56,380
+cell structure أكثر من الفنجاي أكثر من الفيروسات
+
+11
+00:00:56,380 --> 00:00:58,660
+وبيعطوها أهمية أكبر من الحجم الأكبر جدًا
+
+12
+00:00:58,660 --> 00:01:03,180
+للبактерيولوجيا كنا بنعرف قبل اليوم مقاييسنا
+
+13
+00:01:03,180 --> 00:01:09,190
+بالمتر و بالصانتمتر و بالمليمترهذا قدرتنا على ..
+
+14
+00:01:09,190 --> 00:01:13,150
+قدرة العين تبعتنا على تمييز بين نقطتين مختلفتين حد
+
+15
+00:01:13,150 --> 00:01:18,670
+ما نسميها resolution قدرة
+
+16
+00:01:18,670 --> 00:01:23,550
+تمييز العين على نقطتين علينهم نقطتين وانفصلتهم
+
+17
+00:01:23,550 --> 00:01:27,450
+نسميها ال resolution power ويمكن انقلعته في
+
+18
+00:01:27,450 --> 00:01:31,350
+الميكروسكوبي .. في الميكروسكوب الضوءي في العدسات
+
+19
+00:01:31,350 --> 00:01:32,430
+اللي هبقضه resolution
+
+20
+00:01:35,730 --> 00:01:39,970
+وأذكركم بأن الـ size اللي احنا بنشوفه مليميتر صغير
+
+21
+00:01:39,970 --> 00:01:46,090
+خالص فبالك لو بنتكلم عن جزء من الف من المليميتر
+
+22
+00:01:46,090 --> 00:01:52,050
+اللي هو ال micro meter لن نراه لن نراه طيب لو كان
+
+23
+00:01:52,050 --> 00:01:56,770
+جزء من عشر أقل ستة مش عشر أقل ثلاثة عشر أقل ستة
+
+24
+00:01:56,770 --> 00:02:00,990
+يعني واحد من مليون من المليميتر لن نراه أبدا حتى
+
+25
+00:02:00,990 --> 00:02:04,470
+بالمايكروسكوب العادى إذا نعينا مليمترات
+
+26
+00:02:05,290 --> 00:02:09,610
+المايكروسكوب العادي مايكرونات مايكرون مايكروميتر
+
+27
+00:02:09,610 --> 00:02:13,210
+او مايكرون نفس المعنى مايكروميتر و هو عشر أقص ناقص
+
+28
+00:02:13,210 --> 00:02:17,810
+ثلاث يعني واحد على ألف واحد على ألف ال nanometer
+
+29
+00:02:17,810 --> 00:02:21,930
+واحد على عشر أقص ستة او عشر أقص ناقص ستة يعني
+
+30
+00:02:21,930 --> 00:02:24,950
+وبالتالي واحد على مليون واحد على مليون إذا ال
+
+31
+00:02:24,950 --> 00:02:29,010
+nanometer أصغر من مين؟ من ال micro meter البكتيريا
+
+32
+00:02:29,010 --> 00:02:32,290
+تبعتنا غالبا بنجيزها بال micro meter بال micro
+
+33
+00:02:32,290 --> 00:02:36,080
+meterوبالتالي نستطيع رؤيتها بال light microscope
+
+34
+00:02:36,080 --> 00:02:41,700
+لكن دون تفاصيل، دون تفاصيل، ليش؟ لأن التفاصيل
+
+35
+00:02:41,700 --> 00:02:46,020
+بتكون بال nano meter فتحتاج إلى microscope أقوى
+
+36
+00:02:46,020 --> 00:02:49,320
+اللي هو ال electron microscope، إذا ال size بيكون
+
+37
+00:02:49,320 --> 00:02:53,980
+شعور بالحجم، نتكلم عن millimeter، وين جدش ال
+
+38
+00:02:53,980 --> 00:02:57,580
+millimeter؟ جزء من الإضفر تبعنا، اللي من يدوك
+
+39
+00:02:57,580 --> 00:03:02,440
+بنشوفه، مصبوط؟ تخيلوا أصغر من هذا الجزء ب1000 مرة
+
+40
+00:03:03,990 --> 00:03:07,670
+يبقى بدنا يكون هنا sense of feeling ليه الحجوم و
+
+41
+00:03:07,670 --> 00:03:12,670
+هنشوفه كمان شوية ان شاء الله الكلام عن ال size و
+
+42
+00:03:12,670 --> 00:03:15,330
+ان ال size صغير و ايش الأرقام اللي اتعامل معاها
+
+43
+00:03:15,330 --> 00:03:18,130
+الان نتكلم عن ال morphology ده لاحظتوا يا عزيزي في
+
+44
+00:03:18,130 --> 00:03:22,550
+الابتدائي او الاداني ان البكتيريا اما كروية او
+
+45
+00:03:22,550 --> 00:03:26,630
+عصرية او spiral اللي هي بيقولنا اكساي بسلار او
+
+46
+00:03:26,630 --> 00:03:31,590
+spiral لكن اليوم بدنا ناخد شوية تفاصيل it is not
+
+47
+00:03:31,590 --> 00:03:34,570
+only the morphology that is matterكمان ال
+
+48
+00:03:34,570 --> 00:03:37,650
+arrangement تبعد الخلايا ال arrangement لحظوا هنا
+
+49
+00:03:37,650 --> 00:03:40,850
+في خريتين جانب بعض ملازمين بعض في أنواع من
+
+50
+00:03:40,850 --> 00:03:45,230
+البكتيريا بنشوفها دايما بهذا الشكل فكويس انه نعرف
+
+51
+00:03:45,230 --> 00:03:49,690
+احنا قلنا في التعريف عملية استثناء مين مش لابس
+
+52
+00:03:49,690 --> 00:03:53,530
+نضارة فاكرين المحاضرة السابقة كانت عملية استثناء
+
+53
+00:03:53,530 --> 00:03:57,690
+مين من الخلايا لايمتلك هذه الصفة او مين من الخلايا
+
+54
+00:03:57,690 --> 00:04:01,410
+لايمتلك هذه الصفة خدوا بالك نيجاتيف او positive
+
+55
+00:04:01,410 --> 00:04:08,430
+بالنسبة انها صفةوتستخدم في التعريف بمعنى تمتلك
+
+56
+00:04:08,430 --> 00:04:12,550
+المقدر على إنتاج إنزيم X أو لا تمتلك المقدر على
+
+57
+00:04:12,550 --> 00:04:17,410
+إنتاج إنزيم X هي صفة سواء صفة سلبية أو صفة إيجابية
+
+58
+00:04:17,410 --> 00:04:21,110
+تعتبر صفة وتستخدم في عملية اللي هو الاستثمار
+
+59
+00:04:21,110 --> 00:04:27,130
+دبلوكوكاي ليه هما تنتهي مع بعض دبلوكوكساي في عندي
+
+60
+00:04:27,130 --> 00:04:31,930
+سلسلة يسموها Striptococci
+
+61
+00:04:32,550 --> 00:04:37,910
+في عندي tetrads اللي هي الأربعات Tetrads
+
+62
+00:04:37,910 --> 00:04:44,550
+وفي عندي اللي هو ال clusters أو العناقيد وتسمى
+
+63
+00:04:44,550 --> 00:04:48,650
+Staphylococcus
+
+64
+00:04:48,650 --> 00:04:54,290
+طب هنضيفه كلمة Staphylococci Tetrads مالهاش للأسف
+
+65
+00:04:54,290 --> 00:04:58,030
+يعني مابينفعش معها هذا المقطع لكن Stryptococci
+
+66
+00:04:58,030 --> 00:05:02,080
+وDiplococci بينفع مع هذا المقطعهنلاقي حالنا لو
+
+67
+00:05:02,080 --> 00:05:06,160
+بتكلم على شكل واحد، arrangement مختلفة، أعطتنا
+
+68
+00:05:06,160 --> 00:05:11,240
+أسماء مختلفة، أعطتنا أنواع بكتيريا مختلفة، إذاً،
+
+69
+00:05:11,240 --> 00:05:17,460
+Size، شكل، Arrangement، نيجي للبسلالي، ال rods
+
+70
+00:05:17,460 --> 00:05:21,080
+عندي، في شاف تلاحظين، single cell، خلية لوحدها،
+
+71
+00:05:21,080 --> 00:05:25,220
+مافيش أي ارتباط مع خلايا أخرى، نلاقينا واحد تانية،
+
+72
+00:05:25,220 --> 00:05:29,760
+نوع تاني، تنتين، دبلو بسلالي المرة ده، مش دبلو
+
+73
+00:05:29,760 --> 00:05:33,850
+كوكايةواحد اتنين تلات اربع اذا كنا بمين بال
+
+74
+00:05:33,850 --> 00:05:38,570
+Stripto سميناها Stripto Bacilli هذه حاجة unique هي
+
+75
+00:05:38,570 --> 00:05:44,710
+دبله كوكاي ولا دبله Bacilli ولا Bacilli ولا كوكاية
+
+76
+00:05:44,710 --> 00:05:50,910
+ماخدة شكل بين الاتنين وتسمى Coco Bacilli يعني هي
+
+77
+00:05:50,910 --> 00:05:54,970
+Bacilli لكن ماخدة شكل كوكساية او كوكساية ماخدة شكل
+
+78
+00:05:54,970 --> 00:06:00,980
+Bacilli لكن مدرسناها تحت ال Bacilliيبقى في تصنيفها
+
+79
+00:06:00,980 --> 00:06:06,900
+تنتمي لمن؟ للبسلين طبعا ليش؟ لأن ال structure تبع
+
+80
+00:06:06,900 --> 00:06:12,180
+ال cell wall تبعها أقرب إلى البسلين من الكوكسر في
+
+81
+00:06:12,180 --> 00:06:15,840
+العمود الأخير فينا ال spiral ال spiral ده بتلاحظوا
+
+82
+00:06:15,840 --> 00:06:20,100
+الخلية هذه اللي قدامكم أكام turn فيها أكام لف يعني
+
+83
+00:06:20,100 --> 00:06:25,720
+واحد اثنان ثلاثة هاي الأكواس تبعتها طب تعالوا
+
+84
+00:06:25,720 --> 00:06:31,460
+تشوفوا التاني هذا تحتها واحدأتنين، ثلاثة، أربع،
+
+85
+00:06:31,460 --> 00:06:37,580
+خمسة، ستة، سبعة، تمانية، الكتير التنتين تم تصنيفهم
+
+86
+00:06:37,580 --> 00:06:42,160
+على أنهم إيش Spiral طب هذه لف واحد اللي هي
+
+87
+00:06:42,160 --> 00:06:44,640
+البكتيريا اللي دراستوها أكيد في المدرسة باسم
+
+88
+00:06:44,640 --> 00:06:50,160
+البكتيريا الضمية أو الوعوية اللي هي ال Vibrio خد
+
+89
+00:06:50,160 --> 00:06:57,890
+بالكوا شكلا هذه بنصنفها تحت ال spiral شكلا لكنطبيا
+
+90
+00:06:57,890 --> 00:07:01,490
+مصنف الأحمين تحت ال Basel Light لأنه هي الأقرب في
+
+91
+00:07:01,490 --> 00:07:04,330
+الشكل وفي التركيب لميه مرضه لل Basel Light لكن
+
+92
+00:07:04,330 --> 00:07:08,990
+Basel Light منحنى .. Basel Light منحنى طيب سنحنا
+
+93
+00:07:08,990 --> 00:07:14,150
+الآن تلت شغلات مهمة جدا .. إيش هما؟ واحد .. Size
+
+94
+00:07:14,150 --> 00:07:17,130
+.. وقولوا بالك و أنا سألكوا سؤال بسيط جدا .. و
+
+95
+00:07:17,130 --> 00:07:20,030
+أنتوا صغار .. و أنتوا صغار .. و الأكبر .. لو
+
+96
+00:07:20,030 --> 00:07:23,450
+جسدكوا بالمتر و أنتوا صغار .. هتعطوا مقياس مختلف
+
+97
+00:07:23,450 --> 00:07:27,840
+.. مع أنكوا human .. من وجه النظرلو بدى انجليزكوا
+
+98
+00:07:27,840 --> 00:07:31,560
+بنقول انه your size is age dependent، age
+
+99
+00:07:31,560 --> 00:07:33,800
+dependent، معتمدة على الأمر، والبكتيريا كذلك
+
+100
+00:07:33,800 --> 00:07:38,200
+الأمر، قد تكون البكتيريا صغيرة في الأمر أو قد تكون
+
+101
+00:07:38,200 --> 00:07:43,580
+كبيرة في الأمر فال size بها يختلف age رقم اتنين،
+
+102
+00:07:43,580 --> 00:07:48,180
+لو احنا حطينا البكتيريا في nutrient rich media،
+
+103
+00:07:48,180 --> 00:07:53,650
+وسط غني بالغذاءو نفس البكتيريا نسخة منها حقناها في
+
+104
+00:07:53,650 --> 00:07:57,310
+nutrient poor media هل تعتقد ان ال size تبقى
+
+105
+00:07:57,310 --> 00:08:01,630
+معاكون واحد؟ ان ال size is a variable يعني age
+
+106
+00:08:01,630 --> 00:08:05,030
+dependent و nutrient dependent طب ليش nutrient
+
+107
+00:08:05,030 --> 00:08:09,670
+dependent؟ البكتيريا بدها أكسجين ووفرنا لهاش احنا
+
+108
+00:08:09,670 --> 00:08:13,290
+كمية كافية من الأكسجين برضه انهم تبقى حاجبونين او
+
+109
+00:08:13,290 --> 00:08:20,610
+حاجة تبقى حاجبون أصغربعدين shape وبعدين cell. اللي
+
+110
+00:08:20,610 --> 00:08:24,090
+بيهمني الصحيح انا انه انا اعرف ان البكتيريا بكل
+
+111
+00:08:24,090 --> 00:08:30,450
+بساطة تحت ال microscope can exhibit one of the
+
+112
+00:08:30,450 --> 00:08:33,270
+three forms اللي هم بيقولنا عنهم ال bauxite و
+
+113
+00:08:33,270 --> 00:08:37,270
+البسلاج و ال spiral كمان شغل مهم تانية انه ال
+
+114
+00:08:37,270 --> 00:08:42,210
+arrangement هل هي diploma ولا strepto ولا اللي هي
+
+115
+00:08:42,210 --> 00:08:46,550
+الاستفالة و كده او بالنسبالي البسلاجالجزء الأهم في
+
+116
+00:08:46,550 --> 00:08:50,630
+محاضرتنا اليوم هو البثيلة للسلسلة وان ارجو انه
+
+117
+00:08:50,630 --> 00:08:53,430
+تركزوا معاه كويس لان المحاضرة مسجلة و بتاليا
+
+118
+00:08:53,430 --> 00:08:57,110
+هتقعدوها مرة تانية و تالتا و ال presentation هتكون
+
+119
+00:08:57,110 --> 00:09:00,310
+available و روشية الله على ال group تبعتنا في ال
+
+120
+00:09:00,310 --> 00:09:07,970
+facebook احنا في قاعة في internal stuff يعني طولات
+
+121
+00:09:07,970 --> 00:09:12,350
+و خزانات بالادمين كاميرات و في هنا جدار و ورا
+
+122
+00:09:12,350 --> 00:09:17,370
+الجدار قد يكون هناك أشياء أخرىفي الخلية تبعدناها
+
+123
+00:09:17,370 --> 00:09:21,770
+اللي احنا قاعدين فيها بنقسم الخلية البكتيرية الى
+
+124
+00:09:21,770 --> 00:09:26,370
+internal و external structures اللي مفصل بينهمين
+
+125
+00:09:26,370 --> 00:09:31,670
+الجدار هذا المواضيع تحدثنا على ان البروكايوت تمتلك
+
+126
+00:09:31,670 --> 00:09:37,710
+جدار جدار البكتيريا ما يميزه هو وجود structure
+
+127
+00:09:37,710 --> 00:09:43,070
+اسمه بورتيدو شلايكا لا يوجد في العالم مخلوق غير
+
+128
+00:09:43,070 --> 00:09:48,980
+البكتيريا على حد علمناعنده بيبتيدو إشرايكا إلا
+
+129
+00:09:48,980 --> 00:09:52,920
+البكتيريا يعني النباتات إليها cell wall لكن
+
+130
+00:09:52,920 --> 00:09:55,480
+النباتات مكوّنة ال cell wall تبقى حالمين من
+
+131
+00:09:55,480 --> 00:10:00,540
+cellulose الفطريات إليها برضه cell wall لكن بيختلف
+
+132
+00:10:00,540 --> 00:10:04,440
+في التركيب عن النباتات وعن اللي هو البكتيريا
+
+133
+00:10:04,440 --> 00:10:07,700
+فالبكتيريا ما يمائزها ال بيبتيدو إشرايكا اتفقنا
+
+134
+00:10:07,700 --> 00:10:11,500
+إنه في internal وفي external تعالوا نطلع على ال
+
+135
+00:10:11,500 --> 00:10:15,640
+external إيش طالع من ال cell wall هنا؟ Flagella
+
+136
+00:10:16,190 --> 00:10:21,390
+اللي هي الأصوات طالع منه كمان حاجة ليه المحفظة
+
+137
+00:10:21,390 --> 00:10:24,850
+اللي سمتوها انتوا باللغة العربية سميناها capsule
+
+138
+00:10:24,850 --> 00:10:30,050
+طالع منها كمان advantages اللي هي الزوائد اللي
+
+139
+00:10:30,050 --> 00:10:33,950
+شايفينها هذه الخضرة كمان ايش سميناها دي بالراحة
+
+140
+00:10:33,950 --> 00:10:38,410
+ليست organ motility ليست لها علاقة بالحركة وانما
+
+141
+00:10:38,410 --> 00:10:42,160
+ليه علاقة بالحركة من؟ الفلاجلاتالـ Capsule اللي هو
+
+142
+00:10:42,160 --> 00:10:44,600
+ضايف على التراكيلها و ال .. و ال Pile برضه اللي هو
+
+143
+00:10:44,600 --> 00:10:49,280
+ضايف لكن نعرف هي التراكيل Pile Flagella زي اللي هو
+
+144
+00:10:49,280 --> 00:10:54,000
+ال Capsule نجي للداخل إيش أول حاجة بعد ال cell
+
+145
+00:10:54,000 --> 00:10:59,300
+wall من جوا؟ البوية الدهان اللي هو ال cell
+
+146
+00:10:59,300 --> 00:11:04,540
+membrane طبقة رقيقة طبقة رقيقة من ال lipids من ال
+
+147
+00:11:04,540 --> 00:11:08,100
+lipids و فيها شوية proteins فيها شوية proteins
+
+148
+00:11:08,100 --> 00:11:12,420
+بسموها cell membrane أو cytoplasmicدرستوها بعدين
+
+149
+00:11:12,420 --> 00:11:16,660
+جوا فيه cytoplasm داخل الخلية في cytoplasm في ال
+
+150
+00:11:16,660 --> 00:11:21,800
+cytoplasm في بعض المكونات أهمها ال chromosome اللي
+
+151
+00:11:21,800 --> 00:11:25,160
+جايه منطقة النواء اللي بيسموها ما يشبه النواء
+
+152
+00:11:25,160 --> 00:11:29,580
+نوكليويد لأنها مش نواء حقيقية فمنها نوكليويد وفيها
+
+153
+00:11:29,580 --> 00:11:33,820
+ال chromosome اللي قلنا عنه one circular single
+
+154
+00:11:33,820 --> 00:11:38,680
+chromosome ايش دولة اللي عاملين ال ..الريبوزومات
+
+155
+00:11:38,680 --> 00:11:42,460
+اللي حكينا عنها المرة الماضية انهم 70S، خمسين،
+
+156
+00:11:42,460 --> 00:11:46,520
+ثلاثين، فاكرين؟ وفي اندي باللون الأزرق هنا أو
+
+157
+00:11:46,520 --> 00:11:50,420
+البرمسجي حجم granules، ال granules زي ما احنا
+
+158
+00:11:50,420 --> 00:11:53,280
+عندنا طلغات في البيت، بنحاول نخزن الأكل لسبوع أو
+
+159
+00:11:53,280 --> 00:11:57,260
+سبوعات، البكتيريا بتحاول تخزن بعض أنواع الأغضي أو
+
+160
+00:11:57,260 --> 00:12:03,500
+بعضالمواد لازمها لأغراض محددة بتخزينها في حبيبات
+
+161
+00:12:03,500 --> 00:12:08,120
+بيسميها inclusion bodies تمام؟ أو granular
+
+162
+00:12:08,120 --> 00:12:13,680
+inclusion حبيبات جنة تخزينية إذا صار عندنا فكرة عن
+
+163
+00:12:13,680 --> 00:12:17,780
+التراكيب الخارجي والتراكيب الداخلي بس فيها تركيب
+
+164
+00:12:17,780 --> 00:12:22,440
+مش ذاكره في التدرس ما حال اسمها شكل دائري حال
+
+165
+00:12:22,440 --> 00:12:28,500
+اسمها plasma هل اتطلع تقولها بشكل مبصر؟ لأاتفقنا
+
+166
+00:12:28,500 --> 00:12:32,600
+على التراكيب الداخلي والتراكيب الخارجية للخليج
+
+167
+00:12:32,600 --> 00:12:36,020
+تصنيف
+
+168
+00:12:36,020 --> 00:12:41,960
+غير اللي اتكلمنا فيه اللي هو داخلي وخارجي فيه
+
+169
+00:12:41,960 --> 00:12:45,620
+essential component وفيه special structures تراكيب
+
+170
+00:12:45,620 --> 00:12:50,460
+خاصة غالبا هذه بدونها الخليج لا تستطيع المعيشة
+
+171
+00:12:50,460 --> 00:12:54,330
+البكتيريةهنا ممكن تعيش بدون هذه التراكيب زي
+
+172
+00:12:54,330 --> 00:12:57,790
+الكبسول و الجلة و ال بالاي و ال sports لكن بدون
+
+173
+00:12:57,790 --> 00:13:01,750
+cell wall صعب جدا بدون cell membrane صعب بدون
+
+174
+00:13:01,750 --> 00:13:05,590
+cytoplasm صعب مستحيل بدون nuclear material أيضا
+
+175
+00:13:05,590 --> 00:13:09,630
+مستحيل وصل الصورة إذا صنفناهم على تراكيب أساسية
+
+176
+00:13:09,630 --> 00:13:15,430
+ضرورية و تراكيب ممكن تكون خاصة
+
+177
+00:13:15,430 --> 00:13:20,330
+توقف اللوحة و ركزوا معايا اللي بتتكلمه الآنهو
+
+178
+00:13:20,330 --> 00:13:23,450
+الأهم على الإطلاق في المحاضرة الجزء اللي أنا
+
+179
+00:13:23,450 --> 00:13:27,290
+احترقه اللي هو ال cell wall و ركزوا معاه كويس و
+
+180
+00:13:27,290 --> 00:13:33,150
+بناش كتابة عشان ناخد الفكرة العامة من التركيب تبع
+
+181
+00:13:33,150 --> 00:13:36,650
+ال cell wall تبع البكتيريا اتفقنا رقم واحد انه لا
+
+182
+00:13:36,650 --> 00:13:42,210
+يوجد مخلوقات أخرى سواء البكتيريا تحتوي على تركيب
+
+183
+00:13:42,210 --> 00:13:47,930
+اسمه peptidoglycan مما يتكون ال peptidoglycan
+
+184
+00:13:47,930 --> 00:13:58,780
+بتكون منه انصريننختصرهم نام و ناج نام و ناج ناخد
+
+185
+00:13:58,780 --> 00:14:12,840
+منهم الحرفين هذول لإختصار تاني نمسح
+
+186
+00:14:12,840 --> 00:14:15,660
+النام و الناج و نشوف كيف التركيب
+
+187
+00:14:50,940 --> 00:14:52,340
+مجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمجمج
+
+188
+00:14:52,500 --> 00:14:56,540
+تكتفي بطبقة واحدة من ال usual icon وأنواع أخرى
+
+189
+00:14:56,540 --> 00:15:03,140
+بدها multiple layers المشكلة في اللي فيها اللي لها
+
+190
+00:15:03,140 --> 00:15:08,320
+طبقات متعددة اللي لها طبقات متعددة مابتقدرش تحط
+
+191
+00:15:08,320 --> 00:15:14,260
+طبقة فوق طبقة هكذا لإن بتكون التركيب ضعيف جدا لازم
+
+192
+00:15:14,260 --> 00:15:15,200
+يطلع
+
+193
+00:15:21,290 --> 00:15:26,310
+أربع amino acid و أربع amino acid من ال M و ال M
+
+194
+00:15:26,310 --> 00:15:30,590
+اللي في الطبقتين تمام بيسموهم ايش أربع باللاتيني
+
+195
+00:15:30,590 --> 00:15:35,930
+Tetra Tetra ببتاعت يعني ببتيد مجون من أربع amino
+
+196
+00:15:35,930 --> 00:15:42,670
+acids واضحة صورة بين ال M و ال M و بيربط بينهم
+
+197
+00:15:42,670 --> 00:15:51,950
+another ببتاعة هذا الرابط بنسميه cross linkcross
+
+198
+00:15:51,950 --> 00:15:59,930
+link تخيلوا مافيش cross link الطبقة هتصير
+
+199
+00:15:59,930 --> 00:16:02,230
+collapsed على الطبقة التانية هتنزل على الطبقة
+
+200
+00:16:02,230 --> 00:16:08,870
+التانية ال cell wall فقط واضيفته فقط واضيفته وهناك
+
+201
+00:16:08,870 --> 00:16:13,170
+بعض ال antibiotic تقتل البكتيريا بإنها تمنع تكوين
+
+202
+00:16:13,170 --> 00:16:18,150
+ال cross link وصل الصورة ليش قلتلكوا النقطة
+
+203
+00:16:18,150 --> 00:16:23,240
+المهمة؟لأن تركيب ال cell wall بوضّح لي كيف بعض ال
+
+204
+00:16:23,240 --> 00:16:26,580
+antibiotics بتشتغل و جزء من دراستنا يا بنات احنا
+
+205
+00:16:26,580 --> 00:16:30,400
+لازم نرجع عارفين كيف ال antibiotic بتجوم بوظيفته،
+
+206
+00:16:30,400 --> 00:16:36,020
+تمام؟ ليه الموضوعات الحيوية ليش دموعنا بقولوا عنها
+
+207
+00:16:36,020 --> 00:16:42,300
+قاتلة للبكتيريا؟ قاتلة غالبا للبكتيريا المعتمدة
+
+208
+00:16:42,300 --> 00:16:46,350
+على طبقات متعددة من مينمن الـ Betelgeuse Laika
+
+209
+00:16:46,350 --> 00:16:52,310
+لأنها بتكسر الرابط بين الـ M والـ G بتكسر الطبقة
+
+210
+00:16:52,310 --> 00:16:57,290
+تبع ال Betelgeuse Laika لذلك لذلك معظم التهابات
+
+211
+00:16:57,290 --> 00:17:01,050
+اللي عين بتسببها الـ Gram Negative مش ال Gram
+
+212
+00:17:01,050 --> 00:17:02,610
+Positive طبعا انت مش عارف ايش Gram Negative ولا
+
+213
+00:17:02,610 --> 00:17:08,310
+Gram Positive لسه فبالتالي هنشرح الموضوع احنا
+
+214
+00:17:08,310 --> 00:17:12,650
+قولنا بأن البكتيريا قد تحتل إلى طبقة واحدة وأنواع
+
+215
+00:17:12,650 --> 00:17:17,780
+أخرى قد تحتل إلى مطبقتعالوا نشوف وين فيه تطبيق
+
+216
+00:17:17,780 --> 00:17:22,100
+عملي لهذا الموضوع في عالم ألماني اسمه Christian
+
+217
+00:17:22,100 --> 00:17:26,720
+Gramm عمل صبغة اسمها Gramstein ممكن دول مرض عليكم
+
+218
+00:17:26,720 --> 00:17:31,780
+اللي اسمه Gramstein ال Gramstein عشان نكون واضحين
+
+219
+00:17:31,780 --> 00:17:35,700
+وبساطين نذكر هذا اللون violet وهذا اللون أحمر
+
+220
+00:17:35,700 --> 00:17:39,060
+انتوا Gram Negative وانتوا Gram Positive انتوا
+
+221
+00:17:39,060 --> 00:17:43,580
+ماكنوش دعوةالان جينا صبغنا العالم الجرام صبغ
+
+222
+00:17:43,580 --> 00:17:48,260
+التنين بلون violet اسمه crystal violet البكتيليا
+
+223
+00:17:48,260 --> 00:17:52,820
+في الوضع الطبيعي شفافة عديمة اللون فبتنطس الصبغة
+
+224
+00:17:52,820 --> 00:17:56,980
+بسهولة و خاصة ان البكتيليا بتكون شحنتها سالبة
+
+225
+00:17:56,980 --> 00:18:00,240
+والصبغة اللى بحكي عنها crystal violet شحنتها موجبة
+
+226
+00:18:00,240 --> 00:18:03,060
+بصير فيه نوع من ال attraction الجدد بين التنين
+
+227
+00:18:03,060 --> 00:18:09,040
+فالتنتين بينصبغوا بلون violet برغم ان احنا ضايفين
+
+228
+00:18:09,040 --> 00:18:14,310
+عليهم أيودين يوتإضافة اليود، إيش بتعمل؟ اليود بدخل
+
+229
+00:18:14,310 --> 00:18:17,930
+حجمه صغير و ال crystal violet نوعا ما حجمه صغير
+
+230
+00:18:17,930 --> 00:18:22,590
+لكن لما اليود بيلاقي ال crystal violet جوه برتبط
+
+231
+00:18:22,590 --> 00:18:27,330
+معاه و بيكوّن مُركّب معقد هذا المُركّب المُعقد
+
+232
+00:18:27,330 --> 00:18:32,070
+يصعب خروجه من خلية مرة أخرى و دخل من ثقوب صغيرة و
+
+233
+00:18:32,070 --> 00:18:35,670
+كان حجمه صغير، لكن إيش صار جوه؟ صار كبير لأنه
+
+234
+00:18:35,670 --> 00:18:40,630
+مُركّب معقد، بطل جلسل أكبر فصابغنا و بعدين ثبتنا
+
+235
+00:18:40,630 --> 00:18:45,030
+اللون بالأيودينالأيودين الشمالي هنا مثبت لللون
+
+236
+00:18:45,030 --> 00:18:50,850
+خطوة التالتة الحاسمة ضفنا مادة decolorizer مادة
+
+237
+00:18:50,850 --> 00:18:56,150
+مزيل لللون وهي عبارة عن إيثانول إيثانول بتعرفوه ال
+
+238
+00:18:56,150 --> 00:19:00,710
+إيثانول بينجح بإزالة اللون من ال gram negative ولا
+
+239
+00:19:00,710 --> 00:19:04,470
+ينجح بإزالة اللون من مين؟ من ال gram positive أحنا
+
+240
+00:19:04,470 --> 00:19:09,570
+ماكنناش اللون الأصلي للبكتيريا شفافة يبقى هتش صار
+
+241
+00:19:09,570 --> 00:19:14,600
+فيها اللون هنا رجعت شفافةو هدى ضالة violet ضفنا
+
+242
+00:19:14,600 --> 00:19:19,680
+عليهم صبغة تانى لونها أحمر اسمها سافران هدى
+
+243
+00:19:19,680 --> 00:19:24,080
+البكتيريا اتذكروا انه أزيل اللون منها فلما اجهها
+
+244
+00:19:24,080 --> 00:19:28,580
+صبغة جديدة اختتها هدى فيها صبغة لما حاطينها على
+
+245
+00:19:28,580 --> 00:19:32,740
+صبغة جديدة مااختتهاش لأنه فيها already صبغة يبقى
+
+246
+00:19:32,740 --> 00:19:36,780
+المحصلة النهائية اللى قلوى أرشد تولونكو red و
+
+247
+00:19:36,780 --> 00:19:40,320
+تولونكو violetإذا انتوا جرام بُسطى، فإيش اللوان كم
+
+248
+00:19:40,320 --> 00:19:46,160
+مرة؟ Violet، و اللوان؟ Red، Gram Negative الأمر
+
+249
+00:19:46,160 --> 00:19:50,580
+سهل لإنه قصصي، أنا حددكوا حدوتة، قولتلكوا قصة إن
+
+250
+00:19:50,580 --> 00:19:55,040
+كيف عملنا و إيش اللي صار معاها لكن الآن نجي
+
+251
+00:19:55,040 --> 00:20:00,200
+للتفسير، لماذا البكتيريا هذه فقدت اللوان اللي Gram
+
+252
+00:20:00,200 --> 00:20:04,650
+Negative و البكتيريا الأخرى لم تفقد اللوان؟هنا
+
+253
+00:20:04,650 --> 00:20:10,790
+المحق اللي مقدر الـ cell wall يفسر لنا ليش فقدت و
+
+254
+00:20:10,790 --> 00:20:17,970
+ليش لم تفقد الا طبعا
+
+255
+00:20:17,970 --> 00:20:20,490
+على الصورة هذه اللي قدامنا الصورة هذه بتقول ..
+
+256
+00:20:20,490 --> 00:20:24,990
+بتقول فان دي طبقات متعددة من جدو إجلايكا تخيلوها
+
+257
+00:20:24,990 --> 00:20:30,600
+كالتالي، تعرفوا الشبك المنخل؟حطه منخل و حطه منه مي
+
+258
+00:20:30,600 --> 00:20:35,380
+ايش بيصير؟ بنزل طب حطه منخلين، تلاتة، أربعة، خمسة
+
+259
+00:20:35,380 --> 00:20:39,460
+و جربه .. بتجل و بيصير فيه بطق في الحركة و في
+
+260
+00:20:39,460 --> 00:20:43,480
+صعوبة للسائل في صعوبة .. يعني ممكن ننجل مي فيهم ..
+
+261
+00:20:43,480 --> 00:20:48,020
+ممكن ننجل؟ ما .. إذا ان انا عندي طبقات متعددة
+
+262
+00:20:48,020 --> 00:20:54,720
+بيحاول الصبغة تخرج منها، لكن بتفشل لإن في طبقات
+
+263
+00:20:54,720 --> 00:21:00,470
+متعاقبة من ال ..لكن لو كانت طبقة واحدة سهل تطلع
+
+264
+00:21:00,470 --> 00:21:04,910
+منها الـ Gram Negative على الجهة هذه، فاكرينها؟ لا
+
+265
+00:21:04,910 --> 00:21:07,930
+يرتبط لطبقة واحدة من الدجلايكال بينما ال Gram
+
+266
+00:21:07,930 --> 00:21:12,130
+Positive يرتبط لـ Multiple Layer من اللي هو اللي
+
+267
+00:21:12,130 --> 00:21:15,870
+بده دجلايكال، هذا نقطة أساسية في الفروقات بين
+
+268
+00:21:15,870 --> 00:21:20,630
+الاتنين، بس في كمان نقطة هنا في ال Gram Negative
+
+269
+00:21:20,630 --> 00:21:23,630
+في كمية دهون عالية جدا، طب إيش داخل الدهون في
+
+270
+00:21:23,630 --> 00:21:28,720
+الموضوع؟الدهون موجود على الـ steel wall من برا فال
+
+271
+00:21:28,720 --> 00:21:33,300
+decolourizer اللي هو ايه ثانول يزيل الدهون بيذيبها
+
+272
+00:21:33,300 --> 00:21:37,540
+فلما يذيب الدهون بيصير فيه مسامات أوسع فإمكانية
+
+273
+00:21:37,540 --> 00:21:42,040
+خلول الصفقة كم بتكون ايه؟ أسهل على مقابل ال ground
+
+274
+00:21:42,040 --> 00:21:46,900
+positive كمية الدهون نادرة جدا وبالتالي لما بتدوب
+
+275
+00:21:46,900 --> 00:21:50,500
+مش هتطلق منها مسامات عالية إيه دلوقتي فرق الأنصار
+
+276
+00:21:50,500 --> 00:21:54,270
+هنا؟ فرقين أساسيينmultiple layers of peptido
+
+277
+00:21:54,270 --> 00:21:58,370
+glycan على الجهة هذه وكمية دهون قليلة هنا على
+
+278
+00:21:58,370 --> 00:22:03,150
+الطرف هنا one single layer of peptido glycan وفين
+
+279
+00:22:03,150 --> 00:22:11,230
+دي large amount of lipids
+
+280
+00:22:11,230 --> 00:22:15,030
+هذا الشكل يعني اللي قدامنا توضحلنا صورة يعني
+
+281
+00:22:15,030 --> 00:22:20,470
+توضيحية عن مدى الفرقاء ما بين the ground post and
+
+282
+00:22:20,470 --> 00:22:23,390
+the cell wall envelopeاللي هو cell wall يعني و ال
+
+283
+00:22:23,390 --> 00:22:27,230
+gram negative cell wall اتطلعوا هنا الجدوجلايكان
+
+284
+00:22:27,230 --> 00:22:30,650
+واضح انه عبارة ايه عن multiple layer بينما هنا
+
+285
+00:22:30,650 --> 00:22:35,730
+single layer هنا في حيث ما outer membrane بس في
+
+286
+00:22:35,730 --> 00:22:39,570
+الجرام بوسطي مافيش outer membrane مين أحسن؟ الجرام
+
+287
+00:22:39,570 --> 00:22:43,810
+بوسطي ولا الجرام نيجاتيف؟ مين أكتر فعلية؟ الجرام
+
+288
+00:22:43,810 --> 00:22:50,930
+نيجاتيف ولا الجرام بوسطي؟ مش سامعحد يرفع عيده
+
+289
+00:22:50,930 --> 00:22:59,470
+ويقول جواب عشان اللي بيقول جواب بيفسر ليه شك إياكم
+
+290
+00:22:59,470 --> 00:23:03,170
+أن تنخدعوا في ال microbiology بالمقارنات عندما
+
+291
+00:23:03,170 --> 00:23:07,910
+تسألوا مين أفضل بتكونوا تفكروا كويس طالما أنه
+
+292
+00:23:07,910 --> 00:23:11,630
+اتنين موجودين يبجي هتلاقوا أنه لكل واحد منهم فيه
+
+293
+00:23:11,630 --> 00:23:16,330
+مزايا وفيه عيوب هذا الكلام بينطبق على كل إشي موجود
+
+294
+00:23:16,330 --> 00:23:17,290
+منه اتنين أو أكثر
+
+295
+00:23:20,440 --> 00:23:24,340
+المقارنات بتكون صعبة، مين أحسن؟ مين أحسن في وضع
+
+296
+00:23:24,340 --> 00:23:29,160
+معين؟ الـgram negative شفنا احنا أنه فيها single
+
+297
+00:23:29,160 --> 00:23:36,140
+layer الـsingle layer هذا كافية ومعطي الوظائف
+
+298
+00:23:36,140 --> 00:23:40,440
+الأساسية للـcell wall و الخلية عاشها بأمان و تمام
+
+299
+00:23:40,440 --> 00:23:47,660
+بالعكس هي مقاومة للدموع تبعتناهي مقاومة للبنسالين
+
+300
+00:23:47,660 --> 00:23:51,460
+اللي فاكرين انه بيكسر ال cross-link هي مقاومة لل
+
+301
+00:23:51,460 --> 00:23:55,620
+vancomycin برضه اللي بيكسر ال cross-link يبجأت مش
+
+302
+00:23:55,620 --> 00:23:58,660
+معناه الحاجة انه مافيش فيها multiple layer تمام؟
+
+303
+00:23:58,660 --> 00:24:02,380
+معناه الحاجة ان هي الأضعف لأ مش الأضعف بالعكس
+
+304
+00:24:02,380 --> 00:24:06,200
+هنشوف كمان شوية مزايا لل outer membrane اللي
+
+305
+00:24:06,200 --> 00:24:10,400
+انتروها في الإجرام positive and negative لكن في
+
+306
+00:24:10,400 --> 00:24:16,500
+النهاية هدول عبارة عن تركيبين من خيطين مختلفتينوكل
+
+307
+00:24:16,500 --> 00:24:26,100
+واحد بيلو تراكيب او خدنا نقول مزايا خاصة فيه هذه
+
+308
+00:24:26,100 --> 00:24:28,880
+الصورة بوضح ان ال lysozyme يوم بيقطع تكرير ال
+
+309
+00:24:28,880 --> 00:24:32,780
+alternative units اللي هو ال N و ال M اللي عفونا
+
+310
+00:24:32,780 --> 00:24:35,960
+ال M و ال B بيقطع بينهم ال lysozyme اللي هي الدموع
+
+311
+00:24:35,960 --> 00:24:40,670
+اللي موجودة فيه الدموعdestruction للسلول وعشان
+
+312
+00:24:40,670 --> 00:24:45,070
+قلتلكوا ان ال gram-bosted بتأثر أكتر من مين من ال
+
+313
+00:24:45,070 --> 00:24:48,090
+gram-negative لذلك معظم الاتهابات اللي عيبن بيهم
+
+314
+00:24:48,090 --> 00:24:52,630
+بسببها ال gram-negative ال gram-bosted بتموت من
+
+315
+00:24:52,630 --> 00:24:55,930
+الدموع صح فاللي حاسبه الاتهاب مين؟ ال gram
+
+316
+00:24:55,930 --> 00:24:59,290
+-negative
+
+317
+00:24:59,290 --> 00:25:05,370
+أحد التراكيب المهم جدا في ال gram-bosted بكتيريا
+
+318
+00:25:05,370 --> 00:25:09,820
+حارس انها تايكويكاسيدوالـ Tychoic Acid زي ما مقوله
+
+319
+00:25:09,820 --> 00:25:15,980
+في اللوحة ماخد أو جاي من مكانين واحد جاي من ال
+
+320
+00:25:15,980 --> 00:25:19,100
+cell membrane من تحته، شايفينه؟ اللي دول تبعته؟
+
+321
+00:25:19,100 --> 00:25:23,700
+واحد جاي من ال cell wall اللي جاي من ال cell wall
+
+322
+00:25:23,700 --> 00:25:29,240
+بنطلق عليه هكذا، Tychoic Acid التاني اللي جاي من
+
+323
+00:25:29,240 --> 00:25:33,540
+ال cell membrane بنطلق عليه Lipo-Tychoic Acid Lipo
+
+324
+00:25:33,540 --> 00:25:36,840
+لإنه جاى من ال lipids تمام، Lipo-Tychoic Acid
+
+325
+00:25:37,020 --> 00:25:40,820
+والوظيفة أو وظائف متعددة حنتدرسها لما نتكلم عن ال
+
+326
+00:25:40,820 --> 00:25:47,420
+medical microbiogen chart نيجي الأن للتركيب المهم
+
+327
+00:25:47,420 --> 00:25:51,080
+جدا في ال graph of negative bacteria اللي هو the
+
+328
+00:25:51,080 --> 00:25:57,260
+outer membrane وهذه اللوحة اللي قدامنا تفاصيل في
+
+329
+00:25:57,260 --> 00:26:02,640
+حدا فيكم بتخيل القوام القوام أقصد ال consistency
+
+330
+00:26:02,640 --> 00:26:06,430
+تبع ال cell membrane كيف القوام تبعه يعني؟يعني
+
+331
+00:26:06,430 --> 00:26:11,710
+مثلا صلب زي الورقة يعني اللي بنمزعه و لا زي الجلد
+
+332
+00:26:11,710 --> 00:26:17,430
+تبعنا طري و لا ايش؟ كيف القوام تبعه؟ سننبراني
+
+333
+00:26:17,430 --> 00:26:24,410
+اوضحلكوا كيف القوام تبعه، دي بصحن زيت زيتون، بحط
+
+334
+00:26:24,410 --> 00:26:27,830
+أصبعك في الصحن و امسكيه هك، ايش بيصير في زيت
+
+335
+00:26:27,830 --> 00:26:33,150
+زيتون؟ بنكسبه نصين، تمام؟ و بعد ما احنا أصبعنا
+
+336
+00:26:33,150 --> 00:26:38,710
+يمر، ايش بيعمل؟ ترجع تانيعارفين القوام تبع ال cell
+
+337
+00:26:38,710 --> 00:26:45,770
+membrane أقل شويه كمان من ذتته؟ بس لإنه صغير بيكون
+
+338
+00:26:45,770 --> 00:26:49,750
+متناسب نوعا ما لإن حجمه صغير بيكون متناسب عارفين
+
+339
+00:26:49,750 --> 00:26:53,210
+إيش القوام هذا مهم؟ شفت الحركة تبع الأصبع هنا؟
+
+340
+00:26:53,210 --> 00:26:58,370
+تخيلوا إنه فيه هنا هاي ال cell membrane وهنا فيه
+
+341
+00:26:58,370 --> 00:27:03,270
+channel قناة لاستقبال غذاء معين طب الغذاء إيه عشان
+
+342
+00:27:03,270 --> 00:27:10,320
+تتنم الخلية؟لو صلب هذا متحركش لكن هذا ممكن ينتقل
+
+343
+00:27:10,320 --> 00:27:16,360
+من مكانه إلى مكان أخر تشبيه لذيذ و جيد بيقول ان ال
+
+344
+00:27:16,360 --> 00:27:21,020
+cell membrane هو عبارة عن بروتينات تسبح في بحر من
+
+345
+00:27:21,020 --> 00:27:25,320
+الدهون تسبح يعني بمعنى أخر ايش السباحة بيقدر انتقل
+
+346
+00:27:25,320 --> 00:27:31,120
+من مكان إلى مكان فهو عبارة عن يعني قوام لين تمام و
+
+347
+00:27:31,120 --> 00:27:33,940
+تنتقل فيه بروتينات بحرية نوعا ما
+
+348
+00:27:38,150 --> 00:27:43,550
+أنا بدرس ماليستير ميكروبيولوجي وبدرسهم الصحيح هذا
+
+349
+00:27:43,550 --> 00:27:46,210
+الموضوع اللي هو ال .. ال .. ال .. ال outer
+
+350
+00:27:46,210 --> 00:27:48,290
+membrane of the gram negative في ال clinical
+
+351
+00:27:48,290 --> 00:27:52,590
+microbiology وبياخد الموضوع لحاله لحاله إما محاضرة
+
+352
+00:27:52,590 --> 00:27:56,330
+كاملة تلت ساعات أو محاضرة تانية، ست ساعات، بس حين
+
+353
+00:27:56,330 --> 00:27:59,290
+نتكلم عن الجزئية هذه اللي هي اللي قدامنا، لكن احنا
+
+354
+00:27:59,290 --> 00:28:02,970
+اليوم بدنا ناخد فكرة عامة أنا بقول .. بقولت
+
+355
+00:28:02,970 --> 00:28:08,560
+المعلومة السابقة لأدلل على أهمية هذا التركيبمش
+
+356
+00:28:08,560 --> 00:28:12,620
+بدنا نعقد الأمر لإننا نبسطه اليوم لكن هو مهم ومهم
+
+357
+00:28:12,620 --> 00:28:17,160
+جدا إلنا وده البكتيريا إلنا من ناحية إن احنا ممكن
+
+358
+00:28:17,160 --> 00:28:21,620
+نتعرف على البكتيريا من خلاله وده البكتيريا هو أحد
+
+359
+00:28:21,620 --> 00:28:26,440
+أهم عوامل الإمراض في ال gram-negative بكتيريا وأحد
+
+360
+00:28:26,440 --> 00:28:30,540
+أهم عوامل الخداع في ال gram-negative بكتيريا كيف
+
+361
+00:28:30,540 --> 00:28:36,560
+أهم عوامل الخداع؟ شايفين الجزء هذا؟ لفور
+
+362
+00:28:38,090 --> 00:28:42,450
+هذا بيسموه O-side
+
+363
+00:28:42,450 --> 00:28:53,810
+-chain أو بيسموه O-antigen بس
+
+364
+00:28:53,810 --> 00:28:57,690
+بتعرفوش إيه ال antigen و بتعرفوش Antigen هو الدسم
+
+365
+00:28:57,690 --> 00:29:00,770
+الغريب اللي يدخل دسم الإنسان أو الحيوان، الدسم
+
+366
+00:29:00,770 --> 00:29:03,650
+الإنسان أو الحيوان بيكوّله و تتفاعل من أي شكل
+
+367
+00:29:03,650 --> 00:29:07,250
+أجسام مضابط أو تتفاعل specifically مع هذا ال
+
+368
+00:29:07,250 --> 00:29:12,470
+antigenالان بالنظرية الانتيجينات الفيروسات ال
+
+369
+00:29:12,470 --> 00:29:16,930
+influence او الرشح المفروض لما تصيبنا مرة جسمنا
+
+370
+00:29:16,930 --> 00:29:21,430
+يكون جسمنا مضادة المرة الجاية ممنوع يرجع مرة تانية
+
+371
+00:29:21,430 --> 00:29:24,030
+ولكن واقع الأمر ان احنا بنمرض في ال influenza
+
+372
+00:29:24,030 --> 00:29:30,150
+عشرات المرات تمام خاصة موقع الامتحانات مرارا
+
+373
+00:29:30,150 --> 00:29:34,550
+متكرارا لأنه بيصير في عندي ما يسمى بال antigenic
+
+374
+00:29:34,550 --> 00:29:39,100
+variation التغيرات الانتيجينيةإيش هي تغييرة
+
+375
+00:29:39,100 --> 00:29:44,160
+أنتجينية؟ يعني بيصير طفرة فبتغير تركيب الانتجين
+
+376
+00:29:44,160 --> 00:29:48,660
+إذا تغير تركيب الانتجين جهاز المناعة زي بصمة
+
+377
+00:29:48,660 --> 00:29:54,080
+الكمبيوتر تمام؟ لا يستطيع أن يتعرف على الميكروب من
+
+378
+00:29:54,080 --> 00:29:56,960
+شكله وإنما يتعرف عليه من خلال مين؟ الانتجين
+
+379
+00:29:56,960 --> 00:30:00,600
+وبالتالي إذا تغير الانتجين لن يتعرف عليه، هيتعرف
+
+380
+00:30:00,600 --> 00:30:04,440
+عليه مرة كأنه مخلوق جديد هيكوّله أجسام مضادة مرة،
+
+381
+00:30:04,440 --> 00:30:08,840
+أخرى، يعني هياخد ال course مرة تانيةلو تلكوا أن
+
+382
+00:30:08,840 --> 00:30:12,720
+هذا الجزر اللي هو اسمه Antigen أنه في الـ Gram
+
+383
+00:30:12,720 --> 00:30:17,340
+Negative Bacteria هو المسؤول عن الـ Antigenic
+
+384
+00:30:17,340 --> 00:30:21,080
+Variation التغيير الانتجيني أو التنوع الانتجيني
+
+385
+00:30:21,080 --> 00:30:26,640
+للـ Gram Negative Bacteria بس غير السكر هذا أو هذا
+
+386
+00:30:26,640 --> 00:30:31,320
+أو هذا أو .. أيوة ترتيبه غيري إما ترتيب أو أسنسكر
+
+387
+00:30:31,320 --> 00:30:36,900
+اتغير بسبب الانتجين الجديد وليؤدلعلى ذلك تسمعوا
+
+388
+00:30:36,900 --> 00:30:40,120
+بال E coli ولا ماسمعتوش فيها؟ طيب ده اللي ماسمعش
+
+389
+00:30:40,120 --> 00:30:45,620
+في ال E.coli الانسماء فيها و بعد ذلك هتصير أشهر
+
+390
+00:30:45,620 --> 00:30:49,980
+اسم على الإطلاق لديكم في ال microbiology E.coli او
+
+391
+00:30:49,980 --> 00:30:53,900
+E.coli وهي بكتيريا موردة فيه بشكل طبيعي بأمهات
+
+392
+00:30:53,900 --> 00:30:59,580
+الإنسان أمهات؟ ويعني من أكتر الميكروبات استئناسا
+
+393
+00:30:59,580 --> 00:31:02,600
+البشر بتسلسل فيها بيفحصوها وبيشتغلوا عليها
+
+394
+00:31:02,600 --> 00:31:07,410
+دراساتهم العلميةالـ E.coli المفروض أن الأمر ده
+
+395
+00:31:07,410 --> 00:31:11,570
+بصورة طبيعية في أبعادنا لكن بعض أصناف منها بسبب
+
+396
+00:31:11,570 --> 00:31:15,490
+إسهال فلازم نتميز بين الطبيعية وبين السبب لمين ده
+
+397
+00:31:15,490 --> 00:31:20,050
+الإسهال فميزناهم عن طريق الانتجينات اسمها E.coli
+
+398
+00:31:20,050 --> 00:31:24,170
+O157H6
+
+399
+00:31:24,170 --> 00:31:28,290
+بطل E.coli لحالها سنة أخرى دخلنا في تفاصيل
+
+400
+00:31:28,290 --> 00:31:33,630
+الانتجينات تبعها بدينا نتكلم عن O-antigenوعن H،
+
+401
+00:31:33,630 --> 00:31:36,230
+يعني اللي احنا عارفه الـ O عارفناه لما نقولها هو
+
+402
+00:31:36,230 --> 00:31:39,270
+هذا الجزء من مين؟ من الـ outer، من ال brain، اللي
+
+403
+00:31:39,270 --> 00:31:41,310
+مقولته في ال cell wall، اللي مقولته في الجرام،
+
+404
+00:31:41,310 --> 00:31:46,950
+negative، and back to you لأن ماذا تالي O157؟ أول
+
+405
+00:31:46,950 --> 00:31:52,270
+أنتجين، أول أنتجين سمناه O1، اتغير تغيير طفيف، مش
+
+406
+00:31:52,270 --> 00:31:58,250
+حاجة أسميه أنا B، هسميه O2، لإن أنا كلمة واحد
+
+407
+00:31:58,250 --> 00:32:04,420
+تالت، نوع تالت، O3ضلنا نعد و نسجل لما وصلنا لمين؟
+
+408
+00:32:04,420 --> 00:32:09,000
+طبعا في أرقام أعلى من ذلك الأن، هدا بنتكلم عن مين؟
+
+409
+00:32:09,000 --> 00:32:15,800
+عن Elysia coli واحدة صنف واحد استطاعت أن تكتسب
+
+410
+00:32:15,800 --> 00:32:22,080
+أنتجينات أو تنوع أنتجيني خلّى منها مئات السللات،
+
+411
+00:32:22,080 --> 00:32:28,720
+مئات السللات فقط عشان أنها مين؟ أو side-chain أو
+
+412
+00:32:28,720 --> 00:32:34,090
+أو أنتجينالـ O-antigen له أهمية للبكتيريا بإنه
+
+413
+00:32:34,090 --> 00:32:37,030
+أعطاه قدرة على الـ Antigenic Variation طب مش
+
+414
+00:32:37,030 --> 00:32:40,450
+أهميته بالنسبالي أنا كمختبر ك Microbiologist
+
+415
+00:32:40,450 --> 00:32:45,930
+أهميته إنه أنا تعرفت على البكتيريا من خلالها،
+
+416
+00:32:45,930 --> 00:32:49,610
+بمعنىأنا عزلت بكتيريا بدّي أعرف هل هي O157 ولا مش
+
+417
+00:32:49,610 --> 00:32:53,650
+O157 بجيب أجسام مضاطرة antibodies و بحطها على
+
+418
+00:32:53,650 --> 00:32:57,770
+البكتيريا اللي اتفعلت معاها نعم هي O157 ما اتفعلت
+
+419
+00:32:57,770 --> 00:33:02,610
+معاها مش O157 يبقى أنا استخدمت الآن معرفة بالـ O
+
+420
+00:33:02,610 --> 00:33:06,750
+-antigen في التعرف على البكتيريا و يوفر طول
+
+421
+00:33:06,750 --> 00:33:14,590
+النهاردة معرفة جيدة طيب
+
+422
+00:33:14,590 --> 00:33:17,180
+احنا بتكلم .. قاعدين أنا عامل ..عن الـ Outer
+
+423
+00:33:17,180 --> 00:33:22,240
+Membrane of Gram Negative Bacteria مكوّن من
+
+424
+00:33:22,240 --> 00:33:28,840
+Lipopolysaccharide LBS الـ Outer Membrane مكوّن من
+
+425
+00:33:28,840 --> 00:33:32,300
+مين بيقولنا؟ من Lipopolysaccharide هنقولكوا من وين
+
+426
+00:33:32,300 --> 00:33:35,520
+جاي ال Lipopolysaccharide طلع ليه هنا، هاي ال cell
+
+427
+00:33:35,520 --> 00:33:43,240
+wall، هاي ال lipid، فيه انتحار اسمها core يا
+
+428
+00:33:43,240 --> 00:33:49,850
+ده اسم ال lipid ايه؟وهذا core polysaccharide
+
+429
+00:33:49,850 --> 00:33:56,970
+وهذا اسمه O side chain اللي حكينا عنه قبل هو الـ O
+
+430
+00:33:56,970 --> 00:34:00,850
+أنتجين نفس الاسم اللي بقانونيه على الصحيح شغل تاني
+
+431
+00:34:00,850 --> 00:34:04,710
+في هذا التركيب اللي هو الـ O side chain واللي
+
+432
+00:34:04,710 --> 00:34:08,750
+بدأيه ال core تقريبا متشابه فيهم كلهم وبالتالي هو
+
+433
+00:34:08,750 --> 00:34:14,410
+مجرد مسافةتفصل لبث A عن من؟ عن ال O-sided chain
+
+434
+00:34:14,410 --> 00:34:18,870
+مسافة التي طبعاً لها هميتها لكن ليس في هذا المؤشر
+
+435
+00:34:18,870 --> 00:34:24,390
+اللي احنا نتكلم فيها اللبث A اللي هو اسم تاني يا
+
+436
+00:34:24,390 --> 00:34:28,130
+بنت لازم نعرفه الآن و نحفظه كويس اسمه endotoxin
+
+437
+00:34:28,130 --> 00:34:34,130
+اندو؟
+
+438
+00:34:34,130 --> 00:34:39,830
+ايش يعني اندو؟يعني سم داخلي، إيش يعني سم داخلي؟
+
+439
+00:34:39,830 --> 00:34:44,990
+يعني هو تصنيف لهذا السم على إنه هو جزء لا يتجزأ من
+
+440
+00:34:44,990 --> 00:34:48,850
+تكوين الخلية، integral part أقدر أسألك، لا يتجزأ
+
+441
+00:34:48,850 --> 00:34:53,010
+من الخلية، endotoxin، لأن ما أتوقع الخلية هي حية
+
+442
+00:34:53,010 --> 00:34:56,630
+ما تطلق هذا ال toxin، لأنه جزء من تكوينها، حد
+
+443
+00:34:56,630 --> 00:35:01,070
+برمي، بمسك إيده و بقطعها و ما فيها سلاح، لأ، لكن
+
+444
+00:35:01,070 --> 00:35:06,650
+لو موت إيده تتحلل و كل جسمه متحللفعندما يتحلل
+
+445
+00:35:06,650 --> 00:35:11,610
+الاسم ينطلق من ال endotoxin إذا البكتيريا هي حية
+
+446
+00:35:11,610 --> 00:35:16,670
+مابتنترش endotoxin ماذا بتنتجه؟ وهي ميتة لما تبدأ
+
+447
+00:35:16,670 --> 00:35:22,490
+التحلل وأعطيكم نموذج خطير جدا على هذا أو على هذه
+
+448
+00:35:22,490 --> 00:35:29,410
+الحقيقة مريض تم تشخيصه على أنه عنده septicemia
+
+449
+00:35:29,410 --> 00:35:31,610
+septicemia يعني البكتيريا موجودة في الدم موجودة في
+
+450
+00:35:31,610 --> 00:35:35,820
+أين؟ في الدمعملنا له مظلة و اتعرفنا على البكتيريا
+
+451
+00:35:35,820 --> 00:35:39,440
+و طلع اسمها Escherichia coli وعملنا لها فحص حساسي
+
+452
+00:35:39,440 --> 00:35:42,260
+للموضوع الحيوي لجيناها sensitive لجنتا ميسين
+
+453
+00:35:42,260 --> 00:35:48,580
+فالطبيب قرر يعطي المريض جنتا ميسين البكتيريا ماتت
+
+454
+00:35:48,580 --> 00:35:54,480
+و المريض مات مات المريض بموت البكتيريا البكتيريا
+
+455
+00:35:54,480 --> 00:36:00,730
+ماتت فجأةأطلقت كميات ضخمة من الاندوكسين صار عنده
+
+456
+00:36:00,730 --> 00:36:05,350
+ما يسمى بالـGram Negative Septic Shock Shock يعني
+
+457
+00:36:05,350 --> 00:36:10,310
+صدمة وبتقدر لوقفها لأن الاندوكسين له تأثيرات منها
+
+458
+00:36:10,310 --> 00:36:14,650
+بنزل ال blood pressure بيعمل vasodilatation في
+
+459
+00:36:14,650 --> 00:36:21,270
+الآخر بيعمل shock إذن الاندوكسين أحد أهم عوامل
+
+460
+00:36:21,270 --> 00:36:26,120
+الخطورة في المراطمينالجرام نيجاتيف بكتيري هذا كلام
+
+461
+00:36:26,120 --> 00:36:32,060
+غير موجود في الجرام بوسطى فقط في الجرام نيجر يفجأ
+
+462
+00:36:32,060 --> 00:36:36,720
+أول معلومة عرفتوها انتوا نيجر نعم منها ان كل
+
+463
+00:36:36,720 --> 00:36:40,820
+الجرام نيجاتيف تحتوي على اندوتوكسين بينما الجرام
+
+464
+00:36:40,820 --> 00:36:46,320
+بوسطى لا تحتوي على اندوتوكسين ايش السبب انها تحتوي
+
+465
+00:36:46,320 --> 00:36:48,900
+على اندوتوكسين ان هو عندها أوتر ممبران الأوتر
+
+466
+00:36:48,900 --> 00:36:52,000
+ممبران اللي ماتتكون من live polysaccharideالليبو
+
+467
+00:36:52,000 --> 00:36:54,760
+بوليساكراي ده بعن ايه؟ limit A و core
+
+468
+00:36:54,760 --> 00:36:57,400
+polysaccharide و O side chain ال O side chain
+
+469
+00:36:57,400 --> 00:37:02,900
+فهمتوها هي ال O antigen اللي من خلاله جسمنا بيتعرف
+
+470
+00:37:02,900 --> 00:37:11,420
+على البكتيريا و بيحاول يحاربها، بيحاول يحاربها
+
+471
+00:37:11,420 --> 00:37:17,240
+هاي
+
+472
+00:37:17,240 --> 00:37:19,640
+التراكيب اللي ذكرتوا بيه قبل شوية ال live
+
+473
+00:37:19,640 --> 00:37:22,860
+polysaccharide اللي هو ال LPSخاصة اتصال اللي نسميه
+
+474
+00:37:22,860 --> 00:37:27,100
+LBS اتصال اللي نسميه LBS قدر ما كنا لبد ايه و ال
+
+475
+00:37:27,100 --> 00:37:30,460
+core polysaccharide اللي هو ال O أنتجين و لاحظوا
+
+476
+00:37:30,460 --> 00:37:33,540
+هنا جملاش مكتوبة هنا highly valuable اللي يعني
+
+477
+00:37:33,540 --> 00:37:38,460
+متغير بشكل كبير مما يعطي فرصة أكبر و هذا الأمر صار
+
+478
+00:37:38,460 --> 00:37:41,980
+لنا مقضة على أنه نفسر لماذا نقصد بنفس البكتيريا
+
+479
+00:37:41,980 --> 00:37:45,880
+مرارا و تكرارا واقع الأمر هي مش نفس البكتيريا
+
+480
+00:37:45,880 --> 00:37:49,660
+وإنما فيها شوية modification متعلقة بالأنتجينة
+
+481
+00:37:52,500 --> 00:37:55,680
+نجيب على الـ functions of the cell wall ما هي
+
+482
+00:37:55,680 --> 00:38:02,300
+وضائف ال cell wall اللي ممكن احنا نلقصها في شكل
+
+483
+00:38:02,300 --> 00:38:05,500
+النبات رقم واحد maintaining the cell's
+
+484
+00:38:05,500 --> 00:38:09,500
+characteristic shape هي المسئولة عن شكل الخليدة
+
+485
+00:38:09,500 --> 00:38:12,700
+الأول أول يعني إيه ده campus اللهي ولا كوكساي هي
+
+486
+00:38:12,700 --> 00:38:17,840
+المسئولة لأنها rigid عشان rigid صلبة وزي ما
+
+487
+00:38:17,840 --> 00:38:21,220
+قلتلكوا النباتات بتاخد شكل محدد ولا تتحرك لأنها
+
+488
+00:38:21,220 --> 00:38:26,810
+cell wallطيب countering the effect of osmotic
+
+489
+00:38:26,810 --> 00:38:34,870
+pressures تخيلوا معايا بكتيريا أخدنا بكتيريا تمام
+
+490
+00:38:34,870 --> 00:38:38,150
+و حطناها في السيفون و عملنا لها flushing لقى في
+
+491
+00:38:38,150 --> 00:38:42,990
+السيفون flushing البكتيريا كانت في المختبر مزروعة
+
+492
+00:38:42,990 --> 00:38:46,250
+في media مبسوطة من كيفها و دروفها ممتازة رائعة جدا
+
+493
+00:38:46,250 --> 00:38:51,470
+و ال osmotic pressure ملائم جدا لها لما حطناها في
+
+494
+00:38:51,470 --> 00:38:56,710
+الميه الميه مش فيها ملاحقفصار hypo .. مش hypo ..
+
+495
+00:38:56,710 --> 00:39:00,390
+مش ISO .. صار hypo وبالتالي الآن ضغط ال Osmosis
+
+496
+00:39:00,390 --> 00:39:04,050
+اللي في الميه هيسحب الأملاح من البكتيريا ويموتها و
+
+497
+00:39:04,050 --> 00:39:09,930
+الميه تدخل إلى البكتيريا تنفخها فالان مين المسئول
+
+498
+00:39:09,930 --> 00:39:12,510
+عن حماية البكتيريا في هذه اللحظة الخطيرة في
+
+499
+00:39:12,510 --> 00:39:16,870
+حياتها؟ ال cell wall طيب مع أن السفون ضربناه راحت
+
+500
+00:39:16,870 --> 00:39:20,790
+على المجاري أكرركم الله فيه toxin و فيه osmotic
+
+501
+00:39:20,790 --> 00:39:23,430
+pressures مختلفة في الأخر راحت على البحرانا بتبقى
+
+502
+00:39:23,430 --> 00:39:27,910
+البحر مظبوط في البحر كمية الملح عالية جدا عكس المي
+
+503
+00:39:27,910 --> 00:39:31,570
+اللي حلو اللي كنا نتكلم عنها يبقى المسؤول عن حماية
+
+504
+00:39:31,570 --> 00:39:36,410
+البكتيريا من ال variable osmotic pressures هو
+
+505
+00:39:36,410 --> 00:39:39,970
+الcells providing attachment site for
+
+506
+00:39:39,970 --> 00:39:42,690
+bacteriophages بتسمعوا ان في فيروسات بتصيب
+
+507
+00:39:42,690 --> 00:39:46,910
+البكتيريا الفيروسات اللي بتصيب البكتيريا بيسموها
+
+508
+00:39:46,910 --> 00:39:51,830
+bacteriophage او phages يعني باقتصارة و بالعربي لا
+
+509
+00:39:51,830 --> 00:39:55,920
+قيمات البكتيرياتسمية خاطئة، لكن شاعت وما زالت هي
+
+510
+00:39:55,920 --> 00:39:59,280
+نفس التسمية، لأن اللاقم بيكون أكبر، هل يبقى بيكون
+
+511
+00:39:59,280 --> 00:40:04,460
+أصغر وبتتغدى على الفاكيريا؟ من جميعها، it provides
+
+512
+00:40:04,460 --> 00:40:09,680
+a surface to attach، فاكرين الفاجلة؟ من انطلاعها
+
+513
+00:40:10,930 --> 00:40:13,110
+تخيلوا لو الـ Cell Membrane .. لو الـ Cell
+
+514
+00:40:13,110 --> 00:40:15,710
+Membrane هو الفجر من الفجر .. الفجر كل ما اتحرك ..
+
+515
+00:40:15,710 --> 00:40:19,170
+كل ما اتحرك .. هتهز الـ Cell Membrane و هتفسر ..
+
+516
+00:40:19,170 --> 00:40:24,250
+هتكسر لكن هذه بتوفر و انها rigid platform للفجر ..
+
+517
+00:40:24,250 --> 00:40:29,030
+لإيجاد الفمري أنقلا Play an essential role in cell
+
+518
+00:40:29,030 --> 00:40:32,610
+division مسئولة أو إلها دور أساسي في عملية
+
+519
+00:40:32,610 --> 00:40:36,360
+الانقسام، ده بقى نخليهbe the site of major
+
+520
+00:40:36,360 --> 00:40:38,980
+antigenic determinants of the cell surface مش هى
+
+521
+00:40:38,980 --> 00:40:42,840
+.. احنا قولنا هى الجزء الخارج للخلية أو هى الفاصل
+
+522
+00:40:42,840 --> 00:40:46,240
+ما بين الداخل والخارج وبالتالي عليها بيكون اللى هو
+
+523
+00:40:46,240 --> 00:40:49,800
+الانتيجنات اللى من خلالها جسمنا بتعرف على
+
+524
+00:40:49,800 --> 00:40:55,480
+البكتيريا طيب هناك بكتيريا wall-less wall-less
+
+525
+00:40:55,480 --> 00:41:00,960
+يعني منهاش سلوك اسم والاسم هذا .. انا بتكتبه بخط
+
+526
+00:41:09,340 --> 00:41:15,960
+mycoplasma ال mycoplasma تحفظ هذه هي الوحيدة من
+
+527
+00:41:15,960 --> 00:41:20,720
+البكتيريا اللى تستطيع المعيشة بدون cell wall بدون
+
+528
+00:41:20,720 --> 00:41:25,380
+cell wall تنقسم و تتكاتر و بتعيش في البيئة بدون
+
+529
+00:41:25,380 --> 00:41:30,440
+cell wall اسمها mycoplasma و هي اصغر بمناسبة هي
+
+530
+00:41:30,440 --> 00:41:36,580
+اصغر بكتيريا اسمها اصغر بكتيريا اسمها كم مرة
+
+531
+00:41:36,580 --> 00:41:41,150
+mycoplasmaطب طلعوا معايا ال .. الموضوع من زاوية
+
+532
+00:41:41,150 --> 00:41:47,130
+مختلفة بكتيريا أصلا كلها cell wall حطناها في
+
+533
+00:41:47,130 --> 00:41:50,230
+saline تلاعوا ال saline؟ ال normal saline محلول
+
+534
+00:41:50,230 --> 00:41:54,630
+ملح محلول ملحي اللي ضغطه الأزموز تبقى بوازي ضغطه
+
+535
+00:41:54,630 --> 00:41:57,850
+الأزموز تبقى على الخلايا وحطينا بعد ال .. ال
+
+536
+00:41:57,850 --> 00:42:03,210
+saline بنسالين البنسالين هيدمر ال cell wall تمام؟
+
+537
+00:42:03,210 --> 00:42:06,090
+لكن الخلية مش هتانفجر، عارفين ليه مش هتانفجر؟ إن
+
+538
+00:42:06,090 --> 00:42:10,050
+أنا موجودة في normal salineفقدت ال cell wall لكن
+
+539
+00:42:10,050 --> 00:42:14,290
+تقتل اسموزي الموتوسياب هو بالتالي طالب ايه؟ خلية
+
+540
+00:42:14,290 --> 00:42:17,790
+يمكن انتاج هذا الكلام ولا مش ممكن انتاجه؟ ممكن
+
+541
+00:42:17,790 --> 00:42:21,550
+انتاجه واتسمى الخلية بدون cell wall بنسميها
+
+542
+00:42:21,550 --> 00:42:25,830
+protoplast واحنا واحنا كنا طلاب كنا نعمل
+
+543
+00:42:25,830 --> 00:42:30,970
+protoplast عشان ندمج خليتين مع بعض يعني خلية فيها
+
+544
+00:42:30,970 --> 00:42:35,870
+خصاص معينة وخلية أخرى فيها خصاص فنشيل نجشر ال cell
+
+545
+00:42:35,870 --> 00:42:43,090
+wallو نحطهم مع بعض و نحط مادة لحمة تدمجهم مع بعض و
+
+546
+00:42:43,090 --> 00:42:45,830
+بالتالي نحصل على ما يسمى بـ protoplast fusion عشان
+
+547
+00:42:45,830 --> 00:42:50,310
+نحصل على خلية ده تخصائص جديدة، لذلك يمكن للبكتيريا
+
+548
+00:42:50,310 --> 00:42:56,050
+اللي توفرتلها ظروف فيسيولوجية أن تعيش من غيرها من
+
+549
+00:42:56,050 --> 00:43:00,850
+غير cell wall بإضافة إما lysosomes زي اللي قلنا
+
+550
+00:43:00,850 --> 00:43:03,990
+عنه قبل شوية دموع عادي أو بعض ال antibiotics زي
+
+551
+00:43:03,990 --> 00:43:09,410
+اللي هو ال penicillinلكن خد بالك، لو خرجت إلى
+
+552
+00:43:09,410 --> 00:43:12,910
+البيئة، هتموت، هتموت لإنه بعد ذلك المقضر الأعلى
+
+553
+00:43:12,910 --> 00:43:18,550
+تحمل اللي هو الدقة الاسمازي المتغيرة كمان مراجعين
+
+554
+00:43:18,550 --> 00:43:25,830
+يستخدموه إما enzymes أو antibiotics هذه
+
+555
+00:43:25,830 --> 00:43:31,030
+البكتيريا ممكن نلاقي منها natural و بيسموها ال
+
+556
+00:43:31,030 --> 00:43:36,450
+forms ال forms أطلق على الصحيحيعني أشكال L يعني
+
+557
+00:43:36,450 --> 00:43:41,310
+أشكال L مش .. مش معنى الحاجة إنه هي حملة حرف L لأ
+
+558
+00:43:41,310 --> 00:43:46,530
+في حالة اسمها Lister Institute مؤسسة Lister و أنا
+
+559
+00:43:46,530 --> 00:43:51,570
+في بريطانيا طبعا هذه المؤسسة ال .. ال .. الولماء
+
+560
+00:43:51,570 --> 00:43:55,910
+اللي اكتشفوا هذه البكتيريا سموا الأشكال اللي
+
+561
+00:43:55,910 --> 00:43:58,970
+شافوها اللي بدون .. بدون cell wall على اسم المؤسسة
+
+562
+00:43:58,970 --> 00:44:05,570
+مثلا L forms طبعا الأهل L forms هذه كمان مرةأما
+
+563
+00:44:05,570 --> 00:44:08,830
+بتكون Natural أو Artificial Artificial يعني احنا
+
+564
+00:44:08,830 --> 00:44:12,370
+نصنعها، احنا بنصنعها و بنصنعها لغرض زي ما قلتلكم
+
+565
+00:44:12,370 --> 00:44:20,190
+بنعمل Protoplast Fuel طيب
+
+566
+00:44:20,190 --> 00:44:23,450
+ال cell membrane الأنواع الموجودة هنا كونها ال
+
+567
+00:44:23,450 --> 00:44:28,090
+cell membrane بره ولا جوه؟ جوه على طول بعد ال cell
+
+568
+00:44:28,090 --> 00:44:31,030
+wall إلى الداخل يكون احنا الدهان صح؟ يعني الملاصق
+
+569
+00:44:31,030 --> 00:44:36,460
+لمين؟ ل ال cell wallبيكون ال cell membrane طبعا ال
+
+570
+00:44:36,460 --> 00:44:40,860
+cell membrane من أعظم التراكيب اللي موجودة كنتوا
+
+571
+00:44:40,860 --> 00:44:45,500
+يعرفوا أن ال cell wall صحيح له وظائف و راية لكن ال
+
+572
+00:44:45,500 --> 00:44:48,660
+cell membrane ليها قل عنه تذكروا أن البكتيري
+
+573
+00:44:48,660 --> 00:44:52,580
+مافيهاش mitochondria صح؟ مافيهاش عضيات أصلا
+
+574
+00:44:52,580 --> 00:44:56,360
+مافيهاش organelles اللي بيقوم بوظيفة ال cell
+
+575
+00:44:56,360 --> 00:44:59,160
+membrane عفوا لإن ال mitochondria هو ال cell
+
+576
+00:44:59,160 --> 00:45:03,370
+membrane بيعمل عمية ال oxygen phosphorylationو
+
+577
+00:45:03,370 --> 00:45:08,850
+معظم ال energy requiring functions بتن في ال cell
+
+578
+00:45:08,850 --> 00:45:13,270
+membrane و من ضمن ال cell membrane فى تركيب آخر
+
+579
+00:45:13,270 --> 00:45:19,190
+بنضيفه احنا اللى اسم خاص بأن هو لازم منى من ال
+
+580
+00:45:19,190 --> 00:45:23,770
+cell membrane هى مثلا البكتيريا هى ال cell
+
+581
+00:45:23,770 --> 00:45:27,470
+membrane بنلاقي فجأة ال cell membrane صارت يتثنى
+
+582
+00:45:27,470 --> 00:45:34,080
+بهذا الشكل او بهذا الشكللحظة هذه ال .. ال .. ال ..
+
+583
+00:45:34,080 --> 00:45:34,580
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+584
+00:45:34,580 --> 00:45:34,720
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+585
+00:45:34,720 --> 00:45:35,360
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+586
+00:45:35,360 --> 00:45:36,440
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+587
+00:45:36,440 --> 00:45:40,900
+ال .. ال .. ال .. ال .. ال ..
+
+588
+00:45:40,900 --> 00:45:44,340
+ال ..
+
+589
+00:45:44,340 --> 00:45:45,220
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+590
+00:45:45,220 --> 00:45:45,680
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+591
+00:45:45,680 --> 00:45:45,720
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+592
+00:45:45,720 --> 00:45:45,780
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+593
+00:45:45,780 --> 00:45:45,780
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+594
+00:45:45,780 --> 00:45:45,780
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+595
+00:45:45,780 --> 00:45:45,780
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+596
+00:45:45,780 --> 00:45:54,000
+.. ال .. ال .. ال .. ال .. ال .. ال ..
+
+597
+00:45:54,000 --> 00:45:55,060
+ال
+
+598
+00:46:02,020 --> 00:46:07,620
+والتنيات هذه سطحها مستغل وصار عنده كمان إمكانية
+
+599
+00:46:07,620 --> 00:46:14,620
+storage تخزينه فهذا ال structure بيسموه هذا او هذا
+
+600
+00:46:14,620 --> 00:46:21,260
+بيسموه mesosomes وبنجح
+
+601
+00:46:21,260 --> 00:46:24,720
+في الان ده لحد ان شاء الله نكمل نفس المحاضرة بنفس
+
+602
+00:46:24,720 --> 00:46:27,640
+الأرواح المرة القادمة ان شاء الله ال mesosomes هي
+
+603
+00:46:27,640 --> 00:46:30,180
+النقطة اللي انتهينا عندها ان شاء الله
+
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/3tfi9d7ClCY_postprocess.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/3tfi9d7ClCY_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..8d49affb7159431f2ebf21dd7d36db7d3b34065d
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/3tfi9d7ClCY_postprocess.srt
@@ -0,0 +1,2532 @@
+1
+00:00:20,500 --> 00:00:23,840
+الله الرحمن الرحيم موضوع جديد حنبدأ فيه ان شاء
+
+2
+00:00:23,840 --> 00:00:28,120
+الله اليوم اللي هو ال gram negative بس الله انا من
+
+3
+00:00:28,120 --> 00:00:30,700
+اول ما بتكلم عن ال gram stain قولتلكوا ال gram
+
+4
+00:00:30,700 --> 00:00:35,900
+stain بيقسم البكتيريا الى اربع مجموعات اما gram
+
+5
+00:00:35,900 --> 00:00:40,880
+negative او gram positive و كل من هماباس الله او
+
+6
+00:00:40,880 --> 00:00:44,100
+كوكساي و اعتقد انه المهادرات السابقة اتعرفنا
+
+7
+00:00:44,100 --> 00:00:48,960
+لنوعين احنا اللي هم ال gram negative كوكساي متمثلة
+
+8
+00:00:48,960 --> 00:00:52,900
+في النيسيريا و gram positive كوكساي متمثلة في
+
+9
+00:00:52,900 --> 00:00:57,080
+الاستفيل الكوكس والاستفت الكوكس والانترو كوكسلكن
+
+10
+00:00:57,080 --> 00:01:00,720
+من أول المحاضرات وانا حضرتها وانت تلاقي أضخم
+
+11
+00:01:00,720 --> 00:01:04,540
+مجموعة على الإطلاق هي ال gram negative بس ال lie
+
+12
+00:01:04,540 --> 00:01:08,320
+اللي احنا بتقدرنا اليوم فتوقع انه برضه يعني يكون
+
+13
+00:01:08,320 --> 00:01:13,820
+في كم كبير من المعلومات متعلقة بهذه المجموعة عندنا
+
+14
+00:01:13,820 --> 00:01:17,320
+كمان مرة gram stain gram negative باللون الزهري و
+
+15
+00:01:17,320 --> 00:01:20,860
+الأحمر و gram positive ال gram negative زي ما
+
+16
+00:01:20,860 --> 00:01:22,840
+انتوا شايفين من قسم ال
+
+17
+00:01:27,750 --> 00:01:30,990
+طبعا احنا هتكلم على ال gram negative بس الله نطلع
+
+18
+00:01:30,990 --> 00:01:35,230
+على ال diagram برضه تاني هذا كتير مهم إذا بتلاحظوا
+
+19
+00:01:35,230 --> 00:01:40,350
+أنه ال .. ال diagram مقسم البكتيريا إلى قسمين اللي
+
+20
+00:01:40,350 --> 00:01:44,170
+هم ال oxidized positive بال verbal color و ال
+
+21
+00:01:44,170 --> 00:01:47,390
+oxidized negative اللي هي colorless سواء كده
+
+22
+00:01:47,390 --> 00:01:51,370
+positive أو negative برضه مجسمينها جسمين بالان على
+
+23
+00:01:51,370 --> 00:01:58,020
+ال glucose fermentation خديني على اللوحةناخد
+
+24
+00:01:58,020 --> 00:02:02,080
+الأسماء الأربعة اللي بيقولوا الأخطر، ناخد أي واحدة
+
+25
+00:02:02,080 --> 00:02:03,900
+منهم، enterobacterias على سبيل المثال، ال
+
+26
+00:02:03,900 --> 00:02:07,740
+enterobacterias هي، نبدأ من تحت و نطلع فوق، هي
+
+27
+00:02:07,740 --> 00:02:11,620
+positive للجلوكوز fermentation، negative
+
+28
+00:02:11,620 --> 00:02:15,680
+للoxidase، ولأن هو الرئيسي gram negative، بس الله،
+
+29
+00:02:15,680 --> 00:02:20,000
+ناخد SodomaurusIt is negative glucose fermentation
+
+30
+00:02:20,000 --> 00:02:24,920
+positive for oxidized gram-negative bacilli إذا
+
+31
+00:02:24,920 --> 00:02:29,520
+نستطعنا من خلال تلت صفات تلت صفات ال gram-negative
+
+32
+00:02:29,520 --> 00:02:33,580
+bacilli و ال oxidized و ال glucose fermentation أن
+
+33
+00:02:33,580 --> 00:02:38,340
+نقسم كل gram-negative bacilli إلى أربع مجموعات
+
+34
+00:02:38,340 --> 00:02:42,510
+مشهورة جدا اللي هي الاسينيتوفاكتاتnegative
+
+35
+00:02:42,510 --> 00:02:46,370
+negative ال enterobacterial negative positive ال
+
+36
+00:02:46,370 --> 00:02:49,690
+vibrio positive positive ال pseudomonas positive
+
+37
+00:02:49,690 --> 00:02:53,050
+negative أعتقد مش صعبين نحفظهم و ضروري نحفظهم
+
+38
+00:02:53,050 --> 00:03:00,030
+التصنيف هذا بالذات .. التصنيف هذا بالذات كتير مهم
+
+39
+00:03:00,030 --> 00:03:03,090
+لأنه practical في الآخر بتجيني ground negative بس
+
+40
+00:03:03,090 --> 00:03:06,750
+ال like بتعرف عليها أي من الأربع بالفحصين اللي أنا
+
+41
+00:03:06,750 --> 00:03:08,050
+ذكرتهم بكل سهولة
+
+42
+00:03:10,720 --> 00:03:16,240
+هنبدأ بالمجموعة الأولى انترو بكترياسي عشان نقول ان
+
+43
+00:03:16,240 --> 00:03:19,640
+بكتيريا X هي انترو بكترياسي لبن تبدأ لخمس صفر
+
+44
+00:03:19,640 --> 00:03:24,980
+وبنحفظهم الآن في المحاضرة سهلين جداit is gram
+
+45
+00:03:24,980 --> 00:03:28,540
+negative بس الله هي واحدة رقم اتنين oxidase
+
+46
+00:03:28,540 --> 00:03:31,780
+negative هذي مهم جدا تخيل ما عاملنا الآن على
+
+47
+00:03:31,780 --> 00:03:35,180
+انتهاء الوقت بكترياسي oxidase negative تلاتة
+
+48
+00:03:35,180 --> 00:03:39,440
+جليكوز في الفرن طب هدولة التلاتة انا شفتهم في ال
+
+49
+00:03:39,440 --> 00:03:44,840
+diagram اللي جابل صح اضافت لهم كمان صفتين الصفة
+
+50
+00:03:44,840 --> 00:03:47,500
+الأولى they are all facultative anaerobic they can
+
+51
+00:03:47,500 --> 00:03:53,430
+grow aerobically and anaerobicرقم خمسة they can
+
+52
+00:03:53,430 --> 00:03:57,610
+reduce nitrate اللي هو ال NO3 to nitrite اللي هو
+
+53
+00:03:57,610 --> 00:04:05,070
+ال NO2 إذا عندي خمس صفات رئيسية إذا توفرت في أي
+
+54
+00:04:05,070 --> 00:04:08,170
+ميكروب بطلق عليه لقب enterobacteris
+
+55
+00:04:10,970 --> 00:04:13,710
+فاكولتات في أنا أوروبا they reduce nitrate to
+
+56
+00:04:13,710 --> 00:04:17,210
+nitrate, they are all glucose fermenter, they are
+
+57
+00:04:17,210 --> 00:04:19,970
+all oxidize negative, they are all gram negative
+
+58
+00:04:19,970 --> 00:04:24,430
+بس اللي لإن هناك صفات أخرى إضافية قد تساعدنا
+
+59
+00:04:24,430 --> 00:04:28,750
+بالتعابف عليها if motile لل enterobacterias إذا
+
+60
+00:04:28,750 --> 00:04:31,270
+كانت متحركة هل معناها الحاجة إن في منها متحرك
+
+61
+00:04:31,270 --> 00:04:35,310
+ومنها غير؟ متحرك فإذا كانت متحركة فهي متحركة
+
+62
+00:04:35,310 --> 00:04:40,120
+بperitricus flagellaيعني توزيع الفلاجلة على الخلية
+
+63
+00:04:40,120 --> 00:04:45,120
+اللي بيكون على كل المناطق رقم اتنين they are non
+
+64
+00:04:45,120 --> 00:04:50,940
+-spore-forming bacteria انا يعني المعلومة ده مهتمش
+
+65
+00:04:50,940 --> 00:04:55,500
+فيها كتير ليش؟ لإن انا أصلا بعرفش في كل ال .. في
+
+66
+00:04:55,500 --> 00:04:58,380
+كل ال medical important bacteria إلا دنتين بيكونوا
+
+67
+00:04:58,380 --> 00:05:02,260
+non-spore-forming وبالتالي ما عداهم خلاص هم non
+
+68
+00:05:02,260 --> 00:05:06,890
+-spore-formingهذا ال diagram عشان يسهل يعني انا
+
+69
+00:05:06,890 --> 00:05:10,730
+احفظه هو نفس المعلومات اللي اللي هو حاسسها يقوله
+
+70
+00:05:10,730 --> 00:05:14,930
+كمان مرة بنطلع نطلع حافظينهم it is gram negative
+
+71
+00:05:14,930 --> 00:05:18,390
+بس الله it is oxidase negative because fermented
+
+72
+00:05:18,390 --> 00:05:22,890
+مش كمان؟ فاكوتيتر فاكوتيتر فانقره مقفل motile non
+
+73
+00:05:22,890 --> 00:05:26,130
+motile motile bipyritric asphalidella وقلنا
+
+74
+00:05:26,130 --> 00:05:29,190
+produces
+
+75
+00:05:29,190 --> 00:05:32,750
+nitrate to nitrite هذه المعلومة بدنا نتكلم عنها
+
+76
+00:05:32,750 --> 00:05:38,520
+شويةال N O 3 N O 3 بتحول الى N O 2
+
+77
+00:05:38,520 --> 00:05:43,220
+بالانتروبكترياسي فقط لا غير لكن هناك بعض أنواع من
+
+78
+00:05:43,220 --> 00:05:47,920
+البكتيريا قد تستطيع تحول ال nitrate الى nitrogen
+
+79
+00:05:47,920 --> 00:05:55,420
+تتحول من N O 3 الى N O 2 ومن ثم الى N 2 بس
+
+80
+00:05:55,420 --> 00:06:02,900
+الانتروبكترياسي فقط N O 2هذه الصفة بتميزنا ان
+
+81
+00:06:02,900 --> 00:06:06,720
+انتقل الوقت ليها ساعات الاخريات اللي قادرين ان هم
+
+82
+00:06:06,720 --> 00:06:14,480
+يحولوها لانتوا طيب نجي لجزء الصعب دايما في ال
+
+83
+00:06:14,480 --> 00:06:18,120
+micro اللي هو ال classification قولنا حوالي 29
+
+84
+00:06:18,120 --> 00:06:22,440
+جنرة يعني احنا زمان بجينا اقول 14 جنرة اربعتاش
+
+85
+00:06:22,440 --> 00:06:27,520
+صاروا ديهم 29يعني طبعا اي مايكروب نكتشفه و بيمتلك
+
+86
+00:06:27,520 --> 00:06:31,040
+الخمس صفات اللي احنا حكينا عنهم بنحطهم في تارو
+
+87
+00:06:31,040 --> 00:06:35,820
+بردر لكن هناك مايكروبات اشهر من مايكروبات بمعنى
+
+88
+00:06:35,820 --> 00:06:40,460
+انه اكتر شيوعا او احنا اتعرفنا عليها منذ فترة
+
+89
+00:06:40,460 --> 00:06:44,640
+طويلة و دول اصلا بشكل جيد امراضها واضحة انهي اللي
+
+90
+00:06:44,640 --> 00:06:48,400
+اتعامل معاها فبالغالب فبالغالب بتكون اشهر من
+
+91
+00:06:48,400 --> 00:06:54,610
+مجموعات اشهر من مجموعات اخرىعلى اللوحة مكتوب تلت
+
+92
+00:06:54,610 --> 00:06:59,130
+ميكروبات أو تلت genesis باللون الأحمر أو اللون
+
+93
+00:06:59,130 --> 00:07:04,330
+البني يعني اللون مميزة Shigella Salmonella Yersin
+
+94
+00:07:04,330 --> 00:07:09,230
+طب احنا يعني حاطيلهم بلون مختلف نعم لكن جابل ما
+
+95
+00:07:09,230 --> 00:07:12,170
+نتكلم عن اللون المختلف خلينا نتكلم عن فكرة معينة
+
+96
+00:07:14,340 --> 00:07:17,280
+سميه enterobacteriasi، بالك و ليش؟ لأنهم
+
+97
+00:07:17,280 --> 00:07:23,180
+enteromaوي، يعني موجودين في القمعة أو بيعملوا
+
+98
+00:07:23,180 --> 00:07:26,560
+أمراض في القمعة، يبقى واحد من اتنين السبب، إما
+
+99
+00:07:26,560 --> 00:07:29,000
+إنهم موجودين naturally في القمعة أو إنهم بيعملوا
+
+100
+00:07:29,000 --> 00:07:32,200
+infection في القمعة، التلاتة اللي ملونين هدولة مش
+
+101
+00:07:32,200 --> 00:07:36,020
+natural، بيعملوا infection في القمعة، زي السنلة و
+
+102
+00:07:36,020 --> 00:07:37,060
+الشجلة و ال
+
+103
+00:07:49,700 --> 00:07:53,940
+يشيريشيا، سيترو بارتر، كليبسيلة و انترو بارتر، يا
+
+104
+00:07:53,940 --> 00:07:59,360
+ريت هدولة انحط جنبهم حرف ال L لكتوز فرمنتر، لكتوز
+
+105
+00:07:59,360 --> 00:08:07,600
+فرمنتر ال F الباقي
+
+106
+00:08:07,600 --> 00:08:12,080
+ايش مانو الباقي؟ اول حاجة انهم لكتوز فرمنتر كلهم،
+
+107
+00:08:12,080 --> 00:08:14,420
+كلهم، سبع كهر الأب اللي هو الأحمر و ليه غير الأحمر
+
+108
+00:08:15,080 --> 00:08:17,700
+لكن البعض .. non-lectors fundamental البعض منهم
+
+109
+00:08:17,700 --> 00:08:23,920
+normal والبعض منهم بقى فور مشي نقرأهم بشكل سريع
+
+110
+00:08:23,920 --> 00:08:28,340
+عشان يصيروا familiar و نبطل نتغلب في حفظهم طبعا
+
+111
+00:08:28,340 --> 00:08:31,160
+انا بتأكد انه صاروا familiar اصلا عندهم من المعنى
+
+112
+00:08:31,160 --> 00:08:36,460
+اللي نظر الشيليشية شيجيلة إدوارد سيلى و الكلمة
+
+113
+00:08:36,460 --> 00:08:39,600
+الكبيرة اللي ممكنش ننفذها بشكل جيد و نقطعها ده بس
+
+114
+00:08:39,600 --> 00:08:42,520
+إدوارد .. إدوارد اسمه معروف إدوارد سيلى
+
+115
+00:08:50,380 --> 00:08:54,780
+السيراتيا عارفينها السيراتيا الحابرة بروتياس
+
+116
+00:08:54,780 --> 00:08:57,840
+بروفيدنشيا
+
+117
+00:08:57,840 --> 00:09:05,820
+مورجانيلا يرسينيا إيروينيا بكتينوباكتيريا بروتياس
+
+118
+00:09:05,820 --> 00:09:12,740
+بروفيدنشيا مورجانيلا PPM PPM parts per million
+
+119
+00:09:16,200 --> 00:09:19,880
+هدول ال PPM هم groups صحيح؟ group بمعنى ان هم ..
+
+120
+00:09:19,880 --> 00:09:22,960
+انا اخلاق انا قلت انه كويس ان نحطهم مع بعض و
+
+121
+00:09:22,960 --> 00:09:26,800
+نسميهم PPM ده ان هم بيسموهم Protease group مجموعة
+
+122
+00:09:26,800 --> 00:09:30,440
+ال Protease اللي بتكون من ال Protease و ال
+
+123
+00:09:30,440 --> 00:09:35,820
+Providentia و ال Morganium بتميزوا
+
+124
+00:09:35,820 --> 00:09:40,940
+بميزة مهمة جدا اسمها Urease producers they produce
+
+125
+00:09:40,940 --> 00:09:45,320
+large amount of Ureasestrong urease producers
+
+126
+00:09:45,320 --> 00:09:52,320
+المصطلح الدقيق strong urease producers طيب عرفنا
+
+127
+00:09:52,320 --> 00:09:56,440
+بعض الأسماء في ال enterobacteriaceae الان نتكلم عن
+
+128
+00:09:56,440 --> 00:09:59,880
+شكل سريع عن ال unclinic structure طبعا الكلام اللي
+
+129
+00:09:59,880 --> 00:10:05,220
+على اللوحة مافيش كلمة فيه جديدة كل الآن بدنا ندخل
+
+130
+00:10:05,220 --> 00:10:09,100
+لمرحلة التكرار مرحلة الاستفادة من المعلومات اللي
+
+131
+00:10:09,100 --> 00:10:11,880
+درسناها لما اتكلمنا عن ال structure او عن ال
+
+132
+00:10:11,880 --> 00:10:12,300
+metabolism
+
+133
+00:10:20,190 --> 00:10:25,430
+عشان نبدأ نتكلم في ال medical microbiome مالذي
+
+134
+00:10:25,430 --> 00:10:27,590
+يميز ال ground negatives ال wall عن ال ground
+
+135
+00:10:27,590 --> 00:10:30,090
+positives ال wall؟ موجود أوتر من بين، مضبوط لو
+
+136
+00:10:30,090 --> 00:10:32,050
+موجود أوتر من بين ومن اللي مضمن اللي هو ال
+
+137
+00:10:32,050 --> 00:10:37,210
+polysaccharide اللي هو ال polysaccharide فاكرينه
+
+138
+00:10:37,210 --> 00:10:42,790
+اللي بيتقيل؟ ال o-sided chain ال o-sided chain و
+
+139
+00:10:42,790 --> 00:10:48,460
+ال o-ال H-antigen طبعا أنا مرتبط الآن وجوده بمين؟
+
+140
+00:10:48,460 --> 00:10:53,120
+بوجود الفلاجلة، لأن هو الفلاجلة الانتجين، احنا لسه
+
+141
+00:10:53,120 --> 00:10:56,560
+قبل الحلقة ذكرنا، كنا F-motile، motile by
+
+142
+00:10:56,560 --> 00:11:01,560
+perifricous flagella، إذا البكتيريا متحركة، ففيها
+
+143
+00:11:01,560 --> 00:11:03,380
+flagella يعني فيها H-antigen
+
+144
+00:11:07,620 --> 00:11:13,280
+الـ H هو common لكل إجراء من الـ negative بس الـ
+
+145
+00:11:13,280 --> 00:11:16,700
+lie قاطبة انترو بكترياسي أو لا انترو بكترياسي
+
+146
+00:11:16,700 --> 00:11:20,120
+common لكن الـ H مورود فقط في تلك اللتي تحتاج
+
+147
+00:11:20,120 --> 00:11:24,520
+عليها اللي هو السمنة كذلك الأول بالنسبة للتالت
+
+148
+00:11:24,520 --> 00:11:29,900
+اللي هو ال capsular antigen أو ال K انتجن و يطلق
+
+149
+00:11:29,900 --> 00:11:32,700
+عليه لقب VI في حدث سمنه لا
+
+150
+00:11:39,210 --> 00:11:41,990
+فكان مسمين الكابسولة الكهربائي كهربائي كهربائي
+
+151
+00:11:41,990 --> 00:11:42,050
+كهربائي كهربائي كهربائي كهربائي كهربائي كهربائي
+
+152
+00:11:42,050 --> 00:11:44,830
+كهربائي كهربائي كهربائي كهربائي كهربائي كهربائي
+
+153
+00:11:44,830 --> 00:11:48,510
+كهربائي كهربائي كهربائي كهربائي كهربائي كهربائي
+
+154
+00:11:48,510 --> 00:11:51,810
+كهربائي
+
+155
+00:11:51,810 --> 00:11:53,250
+كهربائي كهربائي كهربائي كهربائي كهربائي كهربائي
+
+156
+00:11:53,250 --> 00:11:53,950
+كهربائي كهربائي كهربائي كهربائي كهربائي كهربائي
+
+157
+00:11:53,950 --> 00:11:57,350
+كهربائي كهربائي كهربائي كهربائي كهربائي كهربائي
+
+158
+00:11:57,350 --> 00:12:09,110
+كهربائي كهربائي كهربائ
+
+159
+00:12:09,620 --> 00:12:12,080
+يعني ال O أنتجين هي الدول الموجودة للـ O أنتجين
+
+160
+00:12:12,080 --> 00:12:18,340
+فإن
+
+161
+00:12:18,340 --> 00:12:21,840
+هو أنا معاه Antibody و بدي أكشف عن ال O أنتجين في
+
+162
+00:12:21,840 --> 00:12:25,810
+الصورة هذه، ممكن أكشف عنه؟لازم نشيل ال capsule،
+
+163
+00:12:25,810 --> 00:12:30,110
+لازم نشيل ال capsule، إذا وجود capsule will
+
+164
+00:12:30,110 --> 00:12:33,430
+interfere، إيش هال interfere؟ يتفاعل سلبًا،
+
+165
+00:12:33,430 --> 00:12:37,430
+هيمنعنا من الكشف عن ال O-antigen، إذا بدنا .. بدنا
+
+166
+00:12:37,430 --> 00:12:41,430
+نلاقي طريقة نجشر، نشيل فيها ال capsule، نمسك
+
+167
+00:12:41,430 --> 00:12:44,830
+المكشف اللي بطاطا و نمسك ال bacteria اللي بدنا
+
+168
+00:12:44,830 --> 00:12:47,950
+نجشر فيها، ليش رأيكم؟أحنا قادرين نمسكها الباتريا و
+
+169
+00:12:47,950 --> 00:12:54,710
+نشوفها أصلا ففيها عملية تسخين لمدة نصف ساعة على
+
+170
+00:12:54,710 --> 00:12:58,310
+ستين درجة روية بتدمر اللي هو ال capsule بتدمر ال
+
+171
+00:12:58,310 --> 00:13:02,550
+capsule لإنه يبتكشف عن ال O أنتجين فالكلام مرة
+
+172
+00:13:02,550 --> 00:13:07,370
+صورة اللي قدامنا فيها توضيح لثلاث أنواع من
+
+173
+00:13:07,370 --> 00:13:08,130
+الانتجينات
+
+174
+00:13:12,080 --> 00:13:15,160
+الـ Somatic Antigen أو الـ O Antigen وهو مكوّن من
+
+175
+00:13:15,160 --> 00:13:18,960
+الـ Lipid-A و Polysaccharide core و الـ O side
+
+176
+00:13:18,960 --> 00:13:22,300
+chain و الـ O Antigen بالإضافة إلى قلنا مين اللي
+
+177
+00:13:22,300 --> 00:13:28,020
+هو الـ Calcium طيب احنا ذكرنا ثلاث أنتيجينات
+
+178
+00:13:28,020 --> 00:13:33,060
+رئيسية OHK
+
+179
+00:13:33,060 --> 00:13:39,140
+هل هناك أنتيجينات أخرى نعم أقل أهمية لكن مين المهم
+
+180
+00:13:39,140 --> 00:13:43,530
+أكتر جيبنا ان هم التلات هدولةفهن مثلا أعطر من
+
+181
+00:13:43,530 --> 00:13:48,150
+brain proteins كتيرة متنوعة برضه بتشكل كانتجينات
+
+182
+00:13:48,150 --> 00:13:53,750
+داخل جسم الإنسان ال pillar فاكرين ال pillar في
+
+183
+00:13:53,750 --> 00:13:58,270
+محاضرة في محاضرة نيسيريا جونورية فقناها بالخط
+
+184
+00:13:58,270 --> 00:14:02,390
+العريض لإنه كنا هي الأكتر يعني أو أكتر عامل شراسة
+
+185
+00:14:02,390 --> 00:14:08,130
+للبكتيريا واستغلت البكتيريا في تكوين أنواع مختلفة
+
+186
+00:14:08,130 --> 00:14:09,990
+صح؟ من ال antigens
+
+187
+00:14:15,140 --> 00:14:18,700
+بقولها some are encoded by plasmids يعني البعض
+
+188
+00:14:18,700 --> 00:14:22,320
+منها مش محمولة على كروموزومة واننا محمولة على ال
+
+189
+00:14:22,320 --> 00:14:26,080
+plasmid اللى طبعا كانوا كدرسين للمتر بتعرف انها
+
+190
+00:14:26,080 --> 00:14:29,400
+extra chromosomal self-replicating او
+
+191
+00:14:29,400 --> 00:14:37,340
+independently replicated نعنات messenger طيب
+
+192
+00:14:37,340 --> 00:14:41,760
+قبل ما اقفز على النواحى هذه بدنا نحاول ناخد بعض
+
+193
+00:14:44,370 --> 00:14:48,130
+المعلومات اللي قد تفيدنا لماذا ندرس احنا اصلا اللي
+
+194
+00:14:48,130 --> 00:14:50,890
+هو antigens و لايوجد انتجين او capsule انتجين
+
+195
+00:14:50,890 --> 00:14:55,310
+دايما المعلومات المعلومات اي نوع من المعلومات
+
+196
+00:14:55,310 --> 00:14:58,490
+موجودة قدامنا على الطاولة لو كان في عندي واحد
+
+197
+00:14:58,490 --> 00:15:03,630
+فيزيائي واحد كيميائي واحد طبيب واحد مريض واحد من
+
+198
+00:15:03,630 --> 00:15:08,670
+مجال مختلف كل واحد بينظر للمعلومة من وجه نظره
+
+199
+00:15:08,670 --> 00:15:12,830
+الخاصة يعني لو كنا ما فاقت الانتجينات هنقول من وجه
+
+200
+00:15:12,830 --> 00:15:19,110
+نظر مينالمريض ولا البكتيريا ولا التشخيص اللى هو ال
+
+201
+00:15:19,110 --> 00:15:22,050
+medical microbiology ولا الطبيب كل واحد اللى هو
+
+202
+00:15:22,050 --> 00:15:26,870
+أهداف مختلفة من المعلومة المتعلقة بمين بالانتجنة
+
+203
+00:15:26,870 --> 00:15:32,450
+احنا نبدأ بحالنا microbiologist ايش بنستفيد من
+
+204
+00:15:32,450 --> 00:15:37,510
+الانتجنة تاقول ايه كلها؟ ايش قلت عنها قبل شوية
+
+205
+00:15:37,510 --> 00:15:42,890
+انت؟ مورودة بشكل natural فلقا معاهاطب لو قلتلكوا
+
+206
+00:15:42,890 --> 00:15:45,670
+انا ايكوا والله one five seven eight seven .. اي
+
+207
+00:15:45,670 --> 00:15:49,270
+كم مرة حكيتها هي اليوم اللي كانت؟ كتير صح؟ سابقين؟
+
+208
+00:15:49,270 --> 00:15:52,130
+تلقى معاها برضه؟ طيب لو عزلنا .. اذا انا و احد
+
+209
+00:15:52,130 --> 00:15:55,690
+معاه اسهل و عزلنا من ال stool تبقى اي شي ليه شك
+
+210
+00:15:55,690 --> 00:16:00,030
+والله .. ممكن تكون نوم؟ ممكن تكون نوم؟ ممكن تكون
+
+211
+00:16:00,030 --> 00:16:02,990
+نوم؟ ممكن تكون نوم؟ ممكن تكون نوم؟ ممكن تكون نوم؟
+
+212
+00:16:02,990 --> 00:16:03,390
+ممكن تكون نوم؟ ممكن تكون نوم؟ ممكن تكون نوم؟ ممكن
+
+213
+00:16:03,390 --> 00:16:03,450
+نوم؟ ممكن تكون نوم؟ ممكن تكون نوم؟ ممكن تكون نوم؟
+
+214
+00:16:03,450 --> 00:16:04,230
+ممكن تكون نوم
+
+215
+00:16:09,080 --> 00:16:12,060
+الـ Strain او السلالة more specifically اتعرفنا
+
+216
+00:16:12,060 --> 00:16:15,860
+عليها الان المرحلة اللي نقلناها للطبيب ايش فيها من
+
+217
+00:16:15,860 --> 00:16:21,700
+هو ال body pathogen انها مش normal flora بيس يعني
+
+218
+00:16:21,700 --> 00:16:26,580
+انا استفدت في المقتبر ان اتعرفت بشكل ادق على بنا
+
+219
+00:16:26,580 --> 00:16:31,720
+ال species E coli and strain E coli O157H7 نحن
+
+220
+00:16:31,720 --> 00:16:37,530
+مثال تانيفي أواخر التسعينية للقرن الماضي صار لنا
+
+221
+00:16:37,530 --> 00:16:43,810
+وضع كلها في غزة يعني كلها .. كلها .. الان لو انا
+
+222
+00:16:43,810 --> 00:16:46,630
+بتعمل investigation لل source كلكم بتعرفوا ان كلها
+
+223
+00:16:46,630 --> 00:16:49,650
+تنتقل عن طريق ال water pollution يعني ماء ملوة
+
+224
+00:16:49,650 --> 00:16:51,870
+المياه .. مش كلها مصدر
+
+225
+00:16:56,530 --> 00:17:01,010
+من بلديات، وحتى البلدية الواحدة في المدينة الواحدة
+
+226
+00:17:01,010 --> 00:17:08,870
+قد تزود السكان بمياه ليست مصدر واحد، يعني هما
+
+227
+00:17:08,870 --> 00:17:10,930
+كانوا مصادر مختلفة ومتنوعة، يعني حتى المنطقة اللي
+
+228
+00:17:10,930 --> 00:17:16,810
+كانت غزة كبيرة جالها المنطقة مثلا المخيم أو كده،
+
+229
+00:17:16,810 --> 00:17:20,940
+ففي النهاية أنا بدي أعرف مصدر الماء الملوثلا يعني
+
+230
+00:17:20,940 --> 00:17:26,220
+ان انا عزلت Vibrio من ما يجهي نفس ال Vibrio لعمل
+
+231
+00:17:26,220 --> 00:17:33,380
+الوباءلازم اعرف الانتيجينات للبكتيريا اللى معزولة
+
+232
+00:17:33,380 --> 00:17:38,100
+من الوضع من المرضى يعنى و اعرف الانتيجينات اللى
+
+233
+00:17:38,100 --> 00:17:42,800
+معزولة من العينات البيئية بيها من ضمنها انها اذا
+
+234
+00:17:42,800 --> 00:17:48,140
+توافقت عينات كانتيجينات اللى معزولة من البيئة مع
+
+235
+00:17:48,140 --> 00:17:52,480
+تلك اللى توزنت من المرضى فبيقول هذا most likely is
+
+236
+00:17:52,480 --> 00:17:54,320
+a source of this disease
+
+237
+00:17:58,180 --> 00:18:02,780
+أو نوحي للناس بإنهم يستخدموا او بنكلوا احنا بنقول
+
+238
+00:18:02,780 --> 00:18:05,800
+بإجراءات مثلا بنكلوا هذا الماء اللي بنحط عليه
+
+239
+00:18:05,800 --> 00:18:09,360
+الكلوم يعني بناخد إجراء بين عالمين على مالماتنا من
+
+240
+00:18:09,360 --> 00:18:13,280
+الانتجار هذا الشيء نسميه هنا source tracking إيش
+
+241
+00:18:13,280 --> 00:18:17,200
+أنا source tracking؟ track تتبقى source المصدر
+
+242
+00:18:17,200 --> 00:18:20,920
+يبقى تبقى المصدر في المستشفى .. في المستشفى صاروا
+
+243
+00:18:20,920 --> 00:18:24,720
+بقى في أحد الأقسام و الله افترض الحضارة على أحد
+
+244
+00:18:24,720 --> 00:18:28,440
+الأسافر اللي بيحدثبعض الأحيان، في ال nursery،
+
+245
+00:18:28,440 --> 00:18:31,340
+حبارة اللي هم بيحطوا فيها الأطفال الحديثين الولادة
+
+246
+00:18:31,340 --> 00:18:33,780
+اللي هم غالبا الخدد اللي يقولوا أنهم premature
+
+247
+00:18:33,780 --> 00:18:39,720
+babies، أطفال اللي أعمارهم حمير أقل من 9 شهور أو
+
+248
+00:18:39,720 --> 00:18:44,020
+اللي يقولوا أنهم عندهم إشكالية، فصار النوبة
+
+249
+00:18:44,020 --> 00:18:48,560
+ببكتيريا X، الآن بكتيريا X زي Staphylococcus مثلا
+
+250
+00:18:48,560 --> 00:18:53,160
+أو السيرافيةجينا بنعرف مصدر تبعها خدنا من العاملين
+
+251
+00:18:53,160 --> 00:18:56,180
+لاجينا سيراتيا و سيراتيا و سيراتيا بس ماجياش عندك
+
+252
+00:18:56,180 --> 00:18:59,360
+انت سيراتيا انت مش متهانة خالص لكن ماجياش تهام
+
+253
+00:18:59,360 --> 00:19:03,520
+واحد اتنين تلاتة ماعندهم سيراتيا تفهم الا الا
+
+254
+00:19:03,520 --> 00:19:07,040
+اتأكدنا ان السيراتيا اللى عزلناها من المريض هي نفس
+
+255
+00:19:07,040 --> 00:19:10,100
+السيراتيا اللى عزلناها ولما بقيتم الا عن طريق
+
+256
+00:19:10,100 --> 00:19:13,960
+غالبا الانتجينات نعرف الانتجينات تبعت البقية ايه
+
+257
+00:19:13,960 --> 00:19:17,460
+ده source tracking source tracking is very
+
+258
+00:19:17,460 --> 00:19:21,310
+importantميزنا في المثال الأول ميزنا بين pathogen
+
+259
+00:19:21,310 --> 00:19:25,150
+و non pathogen لما قولنا إيه شريش يا كولاي السؤال
+
+260
+00:19:25,150 --> 00:19:28,010
+على طول كان so what ماهي natural لك لما قولنا إيه
+
+261
+00:19:28,010 --> 00:19:32,450
+كولاي O157H7 يبقى ده أنا صافيا معرفة بين أن هذا
+
+262
+00:19:32,450 --> 00:19:38,270
+السؤال هي pathogen إذا بنا نفهم إنه احنا بالنسبة
+
+263
+00:19:38,270 --> 00:19:43,170
+إنه في ال microbiology نتعرف على ال .. ال .. ال ..
+
+264
+00:19:47,020 --> 00:19:55,040
+أدق لهوية الكائن لأسباب متنوعة مختلفة
+
+265
+00:19:55,040 --> 00:20:01,240
+الانترن بактерياسي ممكن احنا جوناها يعني محترمة في
+
+266
+00:20:01,240 --> 00:20:03,660
+ال intestinal tract او مواطنة محترمة في ال
+
+267
+00:20:03,660 --> 00:20:07,260
+intestinal tract موجودة بشكل طبيعي بالعكس يعني مش
+
+268
+00:20:07,260 --> 00:20:09,740
+بس محترمة لها دور كويس في ال intestinal tract
+
+269
+00:20:14,560 --> 00:20:19,040
+لاتعوي و لا انها normal microbiota ستقوم بعمل
+
+270
+00:20:19,040 --> 00:20:23,660
+infection و أهم واحدة انزل تحت اول واحدة من تحت
+
+271
+00:20:23,660 --> 00:20:27,540
+you urinary tract infection لو تعرف ان erysia coli
+
+272
+00:20:27,540 --> 00:20:30,540
+هي number one cause of urinary tract infection
+
+273
+00:20:33,510 --> 00:20:37,130
+لقيها number one في شغل أكتر من شغل بس من ضمنها it
+
+274
+00:20:37,130 --> 00:20:41,690
+may cause septicemia it may cause pneumonia it may
+
+275
+00:20:41,690 --> 00:20:45,530
+cause meningitis ولا الصحيح اللوحى دى مابدى نفهم
+
+276
+00:20:45,530 --> 00:20:48,730
+منها ان بس هدول هم اللي بيسبوا أوراق ولا مابدى
+
+277
+00:20:48,730 --> 00:20:50,950
+نفهم ان بس هدول هم الأوراق اللي بيسبوا هم هدول
+
+278
+00:20:50,950 --> 00:20:54,910
+المجموعة بس هتعطينا نموذة بقى انه outside the
+
+279
+00:20:54,910 --> 00:20:58,760
+intestinal tract they may cause disease ومنهاإن هو
+
+280
+00:20:58,760 --> 00:21:01,600
+خطير قد يصل إلى اللي هقولنا ال meningitis و
+
+281
+00:21:01,600 --> 00:21:03,880
+الcysticemia و ال pneumonia و ال urinary tract
+
+282
+00:21:03,880 --> 00:21:06,640
+infection طب حتى ال urinary tract infection في
+
+283
+00:21:06,640 --> 00:21:10,120
+منها ال bayonifritis و ال salgilia is a very
+
+284
+00:21:10,120 --> 00:21:13,280
+dangerous one وبالتالي بدنا نكون عارفين إن ال UTI
+
+285
+00:21:13,280 --> 00:21:15,680
+مش حاجة سهلة طيب
+
+286
+00:21:18,110 --> 00:21:21,890
+الانتلا بكتيريا سنة قولت انه نجسمها جسمين، جسم كان
+
+287
+00:21:21,890 --> 00:21:25,910
+reside normally، يعني هو normal flora، و جسم اخر
+
+288
+00:21:25,910 --> 00:21:28,710
+كان cause infection في ال gas في الانتلاك، من
+
+289
+00:21:28,710 --> 00:21:31,830
+الانتلاك .. طبعا كل الانتلاك اللي كانوا موالين
+
+290
+00:21:31,830 --> 00:21:34,590
+باللون البرني فاكرينهم؟ رفارامين
+
+291
+00:21:37,900 --> 00:21:42,880
+مقنع خوفينا هناك لإنه احنا يعني موزعين بين نسميها
+
+292
+00:21:42,880 --> 00:21:46,720
+normal flora ولا البعض .. البعض ثلاث منها اللي
+
+293
+00:21:46,720 --> 00:21:51,320
+بتسبب infection نسميها pathogen فهي الآن صرنا بحق
+
+294
+00:21:51,320 --> 00:21:55,320
+إنه لازم نجوانها pathogen في ال intestinal tract
+
+295
+00:21:55,320 --> 00:21:58,660
+Salmonella, Shigella, Biersinia, Entero, Coelum
+
+296
+00:22:01,160 --> 00:22:03,860
+بنعرف ان ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+297
+00:22:03,860 --> 00:22:03,900
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+298
+00:22:03,900 --> 00:22:03,960
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+299
+00:22:03,960 --> 00:22:04,620
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+300
+00:22:04,620 --> 00:22:05,120
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+301
+00:22:05,120 --> 00:22:05,800
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+302
+00:22:05,800 --> 00:22:06,380
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+303
+00:22:06,380 --> 00:22:11,360
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+304
+00:22:11,360 --> 00:22:15,940
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+305
+00:22:15,940 --> 00:22:16,260
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+306
+00:22:16,260 --> 00:22:16,340
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+307
+00:22:21,210 --> 00:22:25,490
+وجود أي سيروتاعو .. وجود أي سيروتاعو السلمونيلا في
+
+308
+00:22:25,490 --> 00:22:28,390
+الغداء يجعل هذا الغداء غير صاحب الاستهلاك الأناني
+
+309
+00:22:28,390 --> 00:22:36,370
+ما نحاكي على الإطلاق السلمونيلا pathogenic وجودها
+
+310
+00:22:36,370 --> 00:22:41,810
+.. وجودها يجعل الغداء والشراب والطعام أيا كان غير
+
+311
+00:22:41,810 --> 00:22:47,070
+صاحب الاستهلاك الأناني السلمونيلا العقب اللي
+
+312
+00:22:47,070 --> 00:22:49,930
+هحكيلكوا هو أنه نبضة صغيرة جابنا صلها عن
+
+313
+00:22:49,930 --> 00:22:56,130
+السلمونيلاوعن الشجلة، هم لما بنقول سلمانوله أصلا
+
+314
+00:22:56,130 --> 00:23:01,460
+بنقول الشجلة، سلمانوله and شجلةboth has very
+
+315
+00:23:01,460 --> 00:23:04,380
+similar characteristics او يعني very common
+
+316
+00:23:04,380 --> 00:23:07,760
+characteristics منها انهم gram-negative بسلعي،
+
+317
+00:23:07,760 --> 00:23:09,900
+they are enterobacteriasi وكلمة enterobacteriasi
+
+318
+00:23:09,900 --> 00:23:12,800
+يعني كل الصفات المشتركة موجودة، they are non
+
+319
+00:23:12,800 --> 00:23:16,420
+-lactose fermenter، non-lactose fermenter، لكن
+
+320
+00:23:16,420 --> 00:23:19,520
+بتبعتنا بسبب pointestinal tract infection، لكن
+
+321
+00:23:19,520 --> 00:23:23,360
+برضه هناك أوجه اختلاف كبيرة، كبيرة، كبيرة، كبيرة،
+
+322
+00:23:23,360 --> 00:23:26,960
+متعاطف ال diseaseالشيكيلة has never been isolated
+
+323
+00:23:26,960 --> 00:23:30,580
+from sites other than the intestinal tract بمعنى
+
+324
+00:23:30,580 --> 00:23:34,420
+أنه لم .. ما بتسيبش غير ال intestinal tract بس
+
+325
+00:23:34,420 --> 00:23:37,340
+المنلة ممكن أجلبها من one infection، من unary
+
+326
+00:23:37,340 --> 00:23:40,980
+tract، من ال blood، من CSF، من أماكن مختلفة في جسم
+
+327
+00:23:40,980 --> 00:23:45,800
+الإنسان، أمام؟ هذا واحد رقم اتنين يعني بمعنى أخر
+
+328
+00:23:45,800 --> 00:23:50,420
+أنه الشيكيلة بتسيب one disease اللي هواللي هو
+
+329
+00:23:50,420 --> 00:23:53,000
+intestinal tract infection بس Salmonella بتسبب
+
+330
+00:23:53,000 --> 00:23:56,140
+بالاضافة إلى intestinal tract infection أمور أخرى
+
+331
+00:23:56,140 --> 00:24:03,360
+كثيرة الشجلة ماعرفنهاش غير في الإنسان ماتعرفش يعني
+
+332
+00:24:03,360 --> 00:24:08,760
+مخلوق آخر يصاب بالشجلة غير الإنسان يعني نظريا لو
+
+333
+00:24:08,760 --> 00:24:12,640
+دورنا على كل البشر اللي مصابين بالشجلة لو علجناهم
+
+334
+00:24:12,640 --> 00:24:16,740
+سياخد على مين؟ على الشجلة نفسه على الدزيسة على
+
+335
+00:24:16,740 --> 00:24:23,110
+الشجلة نظريابينما السلمونيلا وجدت من العوائل
+
+336
+00:24:23,110 --> 00:24:29,470
+نسميها واحدة reservoir، مخازن، من العوائل ما يعني
+
+337
+00:24:29,470 --> 00:24:36,270
+لا تتخيله، شفت الحردون، السحلية، التمساح، السلاحف،
+
+338
+00:24:36,270 --> 00:24:41,510
+الدواجن، البيض اللي احنا ممكن ناكله ممكن يكون
+
+339
+00:24:41,510 --> 00:24:45,770
+جواته سلمونيلاالمؤامرة بينها وبين الدواجن كبيرة يا
+
+340
+00:24:45,770 --> 00:24:50,250
+بنات ووصلت لإن الدواجن تسمح للسالمونيلا تروح ال
+
+341
+00:24:50,250 --> 00:24:55,030
+ovary وتحقن البيض بخليئة أو خليتان من السالمونيلا
+
+342
+00:24:55,030 --> 00:25:01,190
+وبالتالي انتوا عارفين البيض الآن بيدخل كمنتج في
+
+343
+00:25:01,190 --> 00:25:07,490
+شغلات كتيرة صح ولا لأ طب الدواجن جنة وفيها
+
+344
+00:25:07,490 --> 00:25:14,580
+سالمونيلا الدواجن الآنبنطبخها احنا و الطبخ تبعنا
+
+345
+00:25:14,580 --> 00:25:19,140
+مش على هوا البارد بنسخنها بظبط، بنسخنها اما
+
+346
+00:25:19,140 --> 00:25:22,520
+بالشاوري او بالقلي او بالسلق، بالغالية، بالغالية
+
+347
+00:25:22,520 --> 00:25:26,080
+الميه وهذه غالبا الحرارة بتكون كافية لقتل
+
+348
+00:25:26,080 --> 00:25:30,920
+السلمونيلا إذا الدجاجة نفسها اللي أكلناها حتى لو
+
+349
+00:25:30,920 --> 00:25:34,000
+كان فيها سلمونيلا، غالبا we are protected إلا إذا
+
+350
+00:25:34,000 --> 00:25:37,140
+كان undercooked، undercooked يعني مقلق لم تتعرف
+
+351
+00:25:37,140 --> 00:25:39,780
+لدرجة حرارة كويسة، وانا اتكلم في الشاوري وفي
+
+352
+00:25:39,780 --> 00:25:40,860
+القلي، غالبا
+
+353
+00:25:44,080 --> 00:25:47,720
+الصفحة بتكون عالية لكن في ال center بتكون .. عشانك
+
+354
+00:25:47,720 --> 00:25:51,280
+لو بقى بتفتح حرطة لو لسه .. لسه بنزل دم مش .. يعني
+
+355
+00:25:51,280 --> 00:25:55,580
+مش مستوية ال .. طب وين المشكلة؟ المشكلة في المطبخ
+
+356
+00:25:55,580 --> 00:26:00,820
+واحدة من القطة في اللحم تمام بيحدث ما يسمى cross
+
+357
+00:26:00,820 --> 00:26:04,680
+contamination السكين اللي استخدمناها و الخشب
+
+358
+00:26:04,680 --> 00:26:08,000
+التقطيع ال chopping board اللي استخدمناها هذه الأن
+
+359
+00:26:08,000 --> 00:26:11,750
+أصبحت عليها سالمونةاحنا أخدنا الجولة و أحطناها في
+
+360
+00:26:11,750 --> 00:26:15,290
+الغابة، ما مات سبن اللي هناك بس إنها لها .. لها
+
+361
+00:26:15,290 --> 00:26:18,950
+سكينة و لها chopping board ما matter، غسلت السكينة
+
+362
+00:26:18,950 --> 00:26:22,090
+لسه ليك خفيف أو ال chopping board ما غسلتيها كويس
+
+363
+00:26:22,090 --> 00:26:27,890
+و جيت و عملت بقدونسولا شو .. يعني أي نوع من الأغدى
+
+364
+00:26:27,890 --> 00:26:31,990
+اللي .. اللي لا .. لا يطهر، يعني سلطات، تمام؟
+
+365
+00:26:31,990 --> 00:26:36,770
+فأخدنا السلمونيلا .. أخدنا السلمونيلا من ال .. ال
+
+366
+00:26:36,770 --> 00:26:39,910
+.. ال chiming board أو من السكين وحطناها في غذاء
+
+367
+00:26:39,910 --> 00:26:43,390
+لن يتعرض لحرارة عالية بتاعي سينقذ لنا السلمونيلا،
+
+368
+00:26:43,390 --> 00:26:46,570
+هذا بيسميها حالة cross contamination وهذه الأخطر
+
+369
+00:26:46,570 --> 00:26:53,270
+الصحيح في السلمونيلا، هذا كان اليوم محاضرة تثقيفية
+
+370
+00:26:53,270 --> 00:26:53,850
+في المطبخ
+
+371
+00:26:56,610 --> 00:27:00,030
+بس هذا .. هذا الكلام .. هذا الكلام بيحتم علينا انه
+
+372
+00:27:00,030 --> 00:27:04,770
+دايما فصل الخضروات و ال .. ال .. عن اللحوم، يعني
+
+373
+00:27:04,770 --> 00:27:07,430
+اي شيء متعمد فيه في ال .. في المطبخ، خضروات علينا
+
+374
+00:27:07,430 --> 00:27:11,950
+نفصلها عن اللحوم و الأسماك و ما شابه ده يعني،
+
+375
+00:27:11,950 --> 00:27:15,670
+فإفريشيا، كولاي، سلمونيلا، شيجيلا، يرسينيا، انترو،
+
+376
+00:27:15,670 --> 00:27:16,050
+كل شيء
+
+377
+00:27:19,320 --> 00:27:22,660
+الان ال enterobacteriaceae بدنا ننظر إليها من
+
+378
+00:27:22,660 --> 00:27:27,080
+منظور كمان مرة إن حتى في ال community في المجتمع
+
+379
+00:27:27,080 --> 00:27:32,780
+ممكن نروح المستشفى احنا بأمراض اكتسبناها من
+
+380
+00:27:32,780 --> 00:27:37,480
+المجتمع بتكون نتيجة عن ال enterobacteriaceae مثلا
+
+381
+00:27:37,480 --> 00:27:42,560
+Declepsia pneumonia Declepsia pneumonia بتسبب من
+
+382
+00:27:42,560 --> 00:27:50,430
+اسمها pneumonia وطبعا بتسبب pneumonia وإليهامؤهل
+
+383
+00:27:50,430 --> 00:27:53,170
+لل .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+384
+00:27:53,170 --> 00:27:53,190
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+385
+00:27:53,190 --> 00:27:53,390
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+386
+00:27:53,390 --> 00:27:54,250
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+387
+00:27:54,250 --> 00:27:55,790
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+388
+00:27:55,790 --> 00:27:55,810
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+389
+00:27:55,810 --> 00:27:57,910
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+390
+00:27:57,910 --> 00:27:58,990
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+391
+00:27:58,990 --> 00:28:00,790
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+392
+00:28:00,790 --> 00:28:01,730
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+393
+00:28:01,730 --> 00:28:02,830
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+394
+00:28:02,830 --> 00:28:08,990
+ال .. ال .. ال .. ال .. ال .. ال ..
+
+395
+00:28:08,990 --> 00:28:17,210
+ال .. ال ..
+
+396
+00:28:20,240 --> 00:28:25,940
+Polysaccharide Capsule الورداني ترد انفكشن واللي
+
+397
+00:28:25,940 --> 00:28:28,860
+بتنتج عن غرب على الفيكال Contamination could be
+
+398
+00:28:28,860 --> 00:28:35,600
+caused by E.coli E.coli
+
+399
+00:28:35,600 --> 00:28:39,240
+Clipsilla Enterovacter and Proteus البروتياس إلها
+
+400
+00:28:39,240 --> 00:28:42,560
+ميزة على الواقع فاكرين قبل شوية احنا قولنا بروتياس
+
+401
+00:28:42,560 --> 00:28:47,460
+جروب PPM بروتياس مورجانيلا و البروفيدنش They are
+
+402
+00:28:47,460 --> 00:28:52,540
+all strong UGAsstrong urea is يعني they can
+
+403
+00:28:52,540 --> 00:28:57,360
+convert urea to ammonia and CO2 ammonia is
+
+404
+00:28:57,360 --> 00:29:02,180
+alkaline يبقى ال urine .. ال urine عند الناس
+
+405
+00:29:02,180 --> 00:29:07,540
+المصابين بالبروتيا هيكون ال pH تبعهم alkaline لما
+
+406
+00:29:07,540 --> 00:29:09,840
+تدرسوا ال urine analysis انتوا ان شاء الله هتعرفوا
+
+407
+00:29:09,840 --> 00:29:13,740
+ان ال pH تبع ال urine بيقاس بنجيس ال pH تبع ال
+
+408
+00:29:13,740 --> 00:29:15,920
+urine بيفرق لما يكون acidic على neutral لما يكون
+
+409
+00:29:15,920 --> 00:29:19,660
+ايه؟ alkaline بال strip بال strip صحيح
+
+410
+00:29:27,060 --> 00:29:33,500
+الان ال ph تبع ال urine is essential for some
+
+411
+00:29:33,500 --> 00:29:34,960
+salts
+
+412
+00:29:37,510 --> 00:29:42,130
+اللي احنا عشان بسميهم احنا الأملاح to precipitate
+
+413
+00:29:42,130 --> 00:29:45,030
+في البعض منهم بيصير ال precipitation على high pH
+
+414
+00:29:45,030 --> 00:29:48,050
+والبعض الاخر بيصير ال precipitation على low pH
+
+415
+00:29:48,050 --> 00:29:51,730
+فبالتالي لو كان الأملاح دي موجودة و ال pH alkaline
+
+416
+00:29:51,730 --> 00:29:55,130
+عمال الترسيب بتكون واحدة وبالتالي تكوين حصوات أصلا
+
+417
+00:29:55,130 --> 00:29:59,310
+ككانوكيا بتكون أيضا يعني فنقدر نقول إن ال Protase
+
+418
+00:29:59,310 --> 00:30:04,130
+قد تساهم في تكوين بعض الواعي من ال stones في ال
+
+419
+00:30:04,130 --> 00:30:05,910
+urinary tract
+
+420
+00:30:08,950 --> 00:30:13,050
+ولا مرة ولا بأي حال من الأحوال اللوحة اللي قدامنا
+
+421
+00:30:13,050 --> 00:30:18,330
+هي فقط التدليل على ان ال antibacterial بتسبب
+
+422
+00:30:18,330 --> 00:30:21,710
+community acquired infections اقولكوا من أشهر
+
+423
+00:30:21,710 --> 00:30:24,090
+community acquired infections wound infections
+
+424
+00:30:25,070 --> 00:30:29,090
+تمام؟ بتعرفوا أطفال ان احنا بحبوا الفصل الشتاء و
+
+425
+00:30:29,090 --> 00:30:33,370
+بكيفه لما تكون الشرارة لسه يعني مليئة لميه لكن
+
+426
+00:30:33,370 --> 00:30:37,350
+للأسف ال sewage system انا في قطاع درس زي شفته
+
+427
+00:30:37,350 --> 00:30:41,810
+يعني في شوية أنطار بسيطة يعني صار في بعض المناطق
+
+428
+00:30:41,810 --> 00:30:45,830
+فيها يعني عدم قدرة على الأنظمة ال sewage من
+
+429
+00:30:45,830 --> 00:30:49,730
+الستوار و لتاني صار فينا طوفان في بعض المناطق فلما
+
+430
+00:30:49,730 --> 00:30:54,500
+الأطفال تمشيفي ال .. ال .. هذه المياه اللى الآن
+
+431
+00:30:54,500 --> 00:30:57,500
+اختلطت بال sewage اللى من ضمن ال sewage stool ..
+
+432
+00:30:57,500 --> 00:31:01,080
+stool يعني انتروه بكترياسي من ضمن هاتريشيا كولار
+
+433
+00:31:01,080 --> 00:31:04,280
+لو عنده اي scratch فى ال .. فى ال .. فى المناطق
+
+434
+00:31:04,280 --> 00:31:06,880
+اللى اتعرضت للماء ممكن يصيب عنده wound infection
+
+435
+00:31:06,880 --> 00:31:10,100
+wound infection اذا لان بيبقى عالم ممكن يصيب عنده
+
+436
+00:31:10,100 --> 00:31:13,530
+septicemiaإذا لم يُعرفوا أنهم لديهم meningitis
+
+437
+00:31:13,530 --> 00:31:17,690
+فنعم يعني بمعنى واخر أن ال .. ال .. ال
+
+438
+00:31:17,690 --> 00:31:21,970
+enterovaginase مصدر مهم للعدوى في ال community كما
+
+439
+00:31:21,970 --> 00:31:27,610
+هي مصدر مهم للعدوى في المستشفى نبدأ بالمايكروب
+
+440
+00:31:27,610 --> 00:31:33,790
+الأول اللي هو الشريشيا كولا نبدأ
+
+441
+00:31:33,790 --> 00:31:36,450
+ب .. بعض ال .. ال ..
+
+442
+00:31:42,130 --> 00:31:45,390
+غير انكوا تستحضروا الاحترام والتقديم للبكتيريا،
+
+443
+00:31:45,390 --> 00:31:48,210
+انكوا تذكروا ان ما بتتكلم عن .. ما بتتكلم عن
+
+444
+00:31:48,210 --> 00:31:53,550
+سلالة، بتتكلم عن سلالات، قد واحدة منهم تحتوي على
+
+445
+00:31:53,550 --> 00:31:58,750
+واحد او اكثر من هذه الأراسل، تمام؟ وقد تحتوي على
+
+446
+00:31:58,750 --> 00:32:03,810
+اتنين او تلاتة، اربع، حسب النوع اللي هو السلالة،
+
+447
+00:32:03,810 --> 00:32:09,990
+بتكونوا يعرفوا ان كل ال enterobacteriumبدون ال
+
+448
+00:32:09,990 --> 00:32:15,670
+lipopolysaccharide مابتسوش حاجة بمعنى و الكلام تم
+
+449
+00:32:15,670 --> 00:32:19,150
+تجذيبه انه في بعض الميكروبات أزيل منها ال
+
+450
+00:32:19,150 --> 00:32:23,650
+lipopolysaccharide فقدت قدرتها على ال pathogenesis
+
+451
+00:32:23,650 --> 00:32:28,170
+فال lipopolysaccharide هذا أمر مهم جدا اللي احنا
+
+452
+00:32:28,170 --> 00:32:32,610
+أخدنا من ضمنه أخدنا منه مين فقد ال endotoxin في
+
+453
+00:32:32,610 --> 00:32:36,610
+هذه الحالة كبيرة بالنسبة لك لكن البكتيريا
+
+454
+00:32:37,700 --> 00:32:40,800
+طب .. طب بدي أخلق على النص شويه في المحاضرة و
+
+455
+00:32:40,800 --> 00:32:45,720
+انحاول انه .. برضه نثبت بعض ال .. او مش نثبت ..
+
+456
+00:32:45,720 --> 00:32:50,660
+نحاول انه يعني نقرب بعض الأفكار اللي كانت غريضة
+
+457
+00:32:50,660 --> 00:32:56,300
+لزمان الشيشة كلها طول عمرها مسالمة، natural في ال
+
+458
+00:32:56,300 --> 00:33:00,900
+intestinal flora فجأة لجناها منها سرلات بتنتج أو
+
+459
+00:33:00,900 --> 00:33:04,400
+تمال ديارية و لما دورنا على ال toxin تبعها على
+
+460
+00:33:04,400 --> 00:33:09,520
+جناب بيشبه الشيجل، فسموه shigalو مرة رأى تكسين
+
+461
+00:33:09,520 --> 00:33:14,000
+مشهور كتير اسمه LT تكسين بيشبه الكوليرات، لحظة
+
+462
+00:33:14,000 --> 00:33:19,100
+بيشبه الكوليرات، بيشبه الشجلة، ففاكرين ان الفكرة
+
+463
+00:33:19,100 --> 00:33:24,300
+تبعت انه gene transferإنه J Transfer إنه ممكن
+
+464
+00:33:24,300 --> 00:33:27,720
+بكتيريا هو J Transfer لما بيقولوا conjugation
+
+465
+00:33:27,720 --> 00:33:31,460
+غالبا بيقولوا Species لأ غير Species مع Species
+
+466
+00:33:31,460 --> 00:33:35,640
+بنفس ال Species يعني يعني E coli مع E coli بس الآن
+
+467
+00:33:35,640 --> 00:33:40,020
+واضح من ال .. إنه في .. إنه في إمكانية لانتقال من
+
+468
+00:33:40,020 --> 00:33:43,320
+genus ل genus من genus ل genus يعني مثلا ممكن من
+
+469
+00:33:43,320 --> 00:33:47,300
+Shigella انتقل لمين؟ ل Escherichia coli من
+
+470
+00:33:47,300 --> 00:33:54,370
+Salmonella ل .. او من Vibrio ل Escherichia coliوإن
+
+471
+00:33:54,370 --> 00:34:00,070
+في ممكن مكان يلتقي فيه ميكروفات ومناسب و تتبادل مع
+
+472
+00:34:00,070 --> 00:34:03,450
+بعض الانتجنات، سويش systems، انظمة المجال ال
+
+473
+00:34:03,450 --> 00:34:06,190
+intestinal tract، تمام؟ لو واحد أصيب بسلامه من
+
+474
+00:34:06,190 --> 00:34:10,130
+الله، ما هو ال E.coli موجودة، إمكانية إنه يصير فيه
+
+475
+00:34:10,130 --> 00:34:13,590
+تبادل، الموقع بيكون عالي، قد تنجح وقد تبني، لذلك
+
+476
+00:34:13,590 --> 00:34:19,250
+كل فترة وفترة بنلاقي سلولات جديدة من ال E.coliيعني
+
+477
+00:34:19,250 --> 00:34:23,930
+جامل ممكن تلت سنوات طلعت سولالة فى ألمانيا وصار
+
+478
+00:34:23,930 --> 00:34:27,850
+حتى يعني زعل من أسبانيا تهم .. ألمانيا تهمت
+
+479
+00:34:27,850 --> 00:34:30,730
+أسبانيا أنه من ال .. طماطم المستورد .. او من
+
+480
+00:34:30,730 --> 00:34:33,690
+الخيار المستورد حتى المصريين أطلقوا عليها إعلاميا
+
+481
+00:34:33,690 --> 00:34:36,910
+بكتير الخيار، اه المصريين بيحبوا ي .. لأ و في
+
+482
+00:34:36,910 --> 00:34:42,210
+الآخر طلعت ملخ حراقة بال .. بالأسبانيا، أوروبا
+
+483
+00:34:44,500 --> 00:34:49,540
+أوروبا مرتع لظهور صورة جديدة من اللي هو الاشريشيا
+
+484
+00:34:49,540 --> 00:34:51,920
+كولاي، احنا بالنسبة لإنه يمكن يكون مولود هناك
+
+485
+00:34:51,920 --> 00:34:56,240
+لإنها واخش يعني، بس هم عندهم أدوات ال diagnosis
+
+486
+00:34:56,240 --> 00:35:00,390
+دقيقة وبتالي بيكون في عندهم تتبعيبقى انا اللي
+
+487
+00:35:00,390 --> 00:35:04,150
+بتواصله من الكلام الأخير هذا انه احتمالية انتقال
+
+488
+00:35:04,150 --> 00:35:06,830
+جينات من microbe ل microbe وردة وهذا احد اهم
+
+489
+00:35:06,830 --> 00:35:09,850
+الأسباب اللي دايما بتقولنا انه في ال microbiology
+
+490
+00:35:09,850 --> 00:35:15,230
+مافيش عنها ثبات في انها تغيير باستمرار فمثلا عنه
+
+491
+00:35:15,230 --> 00:35:20,210
+toxins زي shiga type toxin وentelo aggregative ST
+
+492
+00:35:20,210 --> 00:35:23,210
+like toxin ST يعني stable toxin like
+
+493
+00:35:27,240 --> 00:35:33,340
+Hemolysins, endotoxins, type 3 secretion system هو
+
+494
+00:35:33,340 --> 00:35:40,020
+عبارة عن نظام حقن بوصف اسمه molecular syringes
+
+495
+00:35:45,600 --> 00:35:51,220
+فهو أبارعاً سيرنج سيرنج أو مايش بالسيرنج لكن صغيرة
+
+496
+00:35:51,220 --> 00:35:54,560
+جداً ده يسموها molecular syringes البكتيريا
+
+497
+00:35:54,560 --> 00:36:00,920
+بتستخدمها لحق مراكبات إما في بكتيريا أخرى أو في
+
+498
+00:36:00,920 --> 00:36:07,560
+host cell المراكبة هذه بتاع بقى تخيل معايا ال E
+
+499
+00:36:07,560 --> 00:36:10,840
+.coli موجودة في ال intestinal tract على enterocyte
+
+500
+00:36:10,840 --> 00:36:15,570
+على enterocyte خلية من خلال ال intestinal tractال
+
+501
+00:36:15,570 --> 00:36:19,250
+enterocyte مش معنية بلا تعمل phagocytosis لل
+
+502
+00:36:19,250 --> 00:36:25,250
+إيشريشا كولايا مش معنية فالإيشريشا كولايا بتحقق ان
+
+503
+00:36:25,250 --> 00:36:28,910
+فيها مادة بتقولها .. بتوشوشها المادة بتقولها زي
+
+504
+00:36:28,910 --> 00:36:33,410
+ابليسيا بتقولها يعني انت حاجك ناجس طب ما انت ما
+
+505
+00:36:33,410 --> 00:36:36,010
+شاء الله تمتلك مقومات ال macro phage ومبتملك
+
+506
+00:36:36,010 --> 00:36:40,780
+مقومات ال phagocyte كلين انا غذاء جيدتخيلوا
+
+507
+00:36:40,780 --> 00:36:45,640
+بكتيريا الجلة الخالية كليني ايش معناها؟ eat me ايش
+
+508
+00:36:45,640 --> 00:36:49,920
+معناها؟ انها بدها تدخل الى الداخل، بدها تدخل الى
+
+509
+00:36:49,920 --> 00:36:54,880
+الداخل، و لو عارفة حالها هتموت في الداخل، كان
+
+510
+00:36:54,880 --> 00:36:59,480
+مبادرها كليني، كان المبادر ت exist intracellularly
+
+511
+00:37:00,510 --> 00:37:05,330
+فالان ايش اللي بيصير ان السيرنج بتحكم الخلية اللي
+
+512
+00:37:05,330 --> 00:37:09,930
+هو السلب بمواد تعمل حد يسموها polymerization of
+
+513
+00:37:09,930 --> 00:37:13,010
+أكتين بتعمل أكتين تخير معايا الخلية بهذا الشكل
+
+514
+00:37:13,010 --> 00:37:18,950
+اللي مخليها واجفة ومنتفخة ومخت شكلها هو ال
+
+515
+00:37:18,950 --> 00:37:22,650
+cytoskeleton عارفين مما يتكون ال cytoskeleton؟ من
+
+516
+00:37:22,650 --> 00:37:26,870
+الأكتين polymer فبتروح تعمل polymerization للأكتين
+
+517
+00:37:26,870 --> 00:37:30,300
+where الخلية الموجودة هي الموجودة هنا الخليةتخيلوا
+
+518
+00:37:30,300 --> 00:37:34,820
+البوطن هذه الصغيرة، تمام؟ فبيروح بالأكتين بيرفع
+
+519
+00:37:34,820 --> 00:37:38,140
+السجف منها و بيرفع السجف منها و بيصير عنده كذا
+
+520
+00:37:38,140 --> 00:37:42,640
+أقدام كاملة و البكتيريا بتصير في النصر بيبلعها من
+
+521
+00:37:42,640 --> 00:37:48,620
+داخل إلى داخل زي الفاجوزوم و هي في الفاجوزوم الآن،
+
+522
+00:37:48,620 --> 00:37:52,020
+هي موجودة هنا في الفاجوزوم لما البكتيريا تقوم في
+
+523
+00:37:52,020 --> 00:37:56,440
+الفاجوزوم، إيش أول خطوة بيتيجي الآن؟ ال ISO zone
+
+524
+00:37:56,440 --> 00:38:00,830
+عشان يصير الفاجوزوم ال ISO zoneعلى طول بتطلع الـ
+
+525
+00:38:00,830 --> 00:38:04,210
+syringe تبعها و بتحقن ال membrane و بتقول
+
+526
+00:38:04,210 --> 00:38:09,910
+الفاجوزوم false alarm يعني جرس انظر خاطر انت خليك
+
+527
+00:38:09,910 --> 00:38:14,590
+مطرحك مافيش عنا مشاكل خليك مكانك طبعا لما
+
+528
+00:38:14,590 --> 00:38:18,330
+الفاجوزوم مايرتبطش .. يا عفوا الفاجوزوم مايرتبطش
+
+529
+00:38:18,330 --> 00:38:18,990
+في الفاجوزوم
+
+530
+00:38:22,190 --> 00:38:25,770
+بعد ذلك يبدأ
+
+531
+00:38:25,770 --> 00:38:30,010
+البكتيريا تنقسم داخل الخلية انقسام البكتيريا داخل
+
+532
+00:38:30,010 --> 00:38:36,850
+الخلية خد بالك له معاني كثيرة في جهاز المناعم بعيد
+
+533
+00:38:36,850 --> 00:38:37,150
+عن
+
+534
+00:38:41,140 --> 00:38:45,980
+بعيد عن ال antibodies إذا في antibodies لأن جوا
+
+535
+00:38:45,980 --> 00:38:48,320
+الخلية ال antibiotics بتدخل جوا الخلية مش هدخل جوا
+
+536
+00:38:48,320 --> 00:38:51,700
+الخلية صح ولا لأ تركيز ال antibiotics لو في
+
+537
+00:38:51,700 --> 00:38:55,680
+antibiotics في الخلايا أكيد هيكون أقل من تركيزه في
+
+538
+00:38:55,680 --> 00:39:01,940
+الدمفهي محصرة نوعا ما داخل الخلال اللي نبعتنا و
+
+539
+00:39:01,940 --> 00:39:07,180
+تتكاثر و تستخدم الخلية كمادة غدائية لها في التكاثر
+
+540
+00:39:07,180 --> 00:39:12,880
+إذا ال type 3 secretion system نظام رائع جدا لخداء
+
+541
+00:39:12,880 --> 00:39:21,380
+الخلالة و من ثم حق الملكبات يعني لتطوية ال host
+
+542
+00:39:21,380 --> 00:39:23,840
+لصلاة
+
+543
+00:39:25,600 --> 00:39:29,460
+ال .. ال additions و طلع منها اكتر من نوع من ال ..
+
+544
+00:39:29,460 --> 00:39:33,820
+من ال adhesins موجودين في ال ecotype و ان في حوالي
+
+545
+00:39:33,820 --> 00:39:39,860
+واحد و عشرين different types او الا كيف بدنا نفسر
+
+546
+00:39:39,860 --> 00:39:43,690
+.. كيف بدنا نفسر urinary tract infectionالبكتيريا
+
+547
+00:39:43,690 --> 00:39:49,350
+دخلت من ال urethra، من مكان منخفض، و بيطلع فوق عكس
+
+548
+00:39:49,350 --> 00:39:52,310
+التيار، التيار مين؟ ال urethra اللي نزل تحت، صعبة
+
+549
+00:39:52,310 --> 00:39:55,390
+بقى، تطلع و تصل لكيليا، و تعمل bionephritis، كيف
+
+550
+00:39:55,390 --> 00:39:59,370
+بينافسر الكلام؟ بوجود نقدرة هائلة على ال
+
+551
+00:39:59,370 --> 00:40:01,550
+attachment من قبل اللي هو البكتيريا اللي اسمها
+
+552
+00:40:01,550 --> 00:40:05,180
+ischiavaالـ virulence factors that protect the
+
+553
+00:40:05,180 --> 00:40:07,980
+bacteria from most defenses زي الكبسول وال iron
+
+554
+00:40:07,980 --> 00:40:10,920
+capturing ability يعني عندها مقدرة على أنها تبرض
+
+555
+00:40:10,920 --> 00:40:14,760
+ترتبط بمين بالحديد ولا بيساعد على المنافسة معاها
+
+556
+00:40:14,760 --> 00:40:18,300
+اللي هو اللي هو أصلا ايضا في تبتلك outer membrane
+
+557
+00:40:18,300 --> 00:40:21,120
+proteins إذا ان في مشموعة كبيرة من ال virulence
+
+558
+00:40:21,120 --> 00:40:25,080
+factors موجودة في الإشريشيا كلها البعض بقرنة وبعض
+
+559
+00:40:25,080 --> 00:40:28,880
+آخر حد هتكره بالتفصيلة هتكر كل نوع من الإشريشيا
+
+560
+00:40:34,010 --> 00:40:38,550
+بيجاوبنا على أسئلة كثيرة قد تساعدنا في الفهم بعضنا
+
+561
+00:40:38,550 --> 00:40:42,230
+البعض
+
+562
+00:40:42,230 --> 00:40:44,910
+انا براحة قبل ان احضر قطار انا حاطط فيديو على
+
+563
+00:40:44,910 --> 00:40:51,320
+الفيسبوك بمضحك الليستيرياالليستيريا كيف تخترق
+
+564
+00:40:51,320 --> 00:40:57,060
+الخلية إلى خلية أخرى بدل إنها تطلع برا تمام؟ وتعمل
+
+565
+00:40:57,060 --> 00:41:00,360
+infection لخلية تانية؟ ده بيعمل حاجة اسمها actin
+
+566
+00:41:00,360 --> 00:41:04,180
+rockets اللي هي صواريخ باكتين وبتضرب حاجة بتروح
+
+567
+00:41:04,180 --> 00:41:08,860
+ليها على الخلية التانية يارتبوا الحسابة تبعنا في
+
+568
+00:41:08,860 --> 00:41:11,440
+ال essential medical micro وفي الفيسبوك لإنه أنا
+
+569
+00:41:11,440 --> 00:41:15,100
+كل فترة فترة بضيف يعني بعض الأشياء الجاية المناسبة
+
+570
+00:41:15,480 --> 00:41:18,160
+هذه الصورة اللي كتبتها قبل هيك اللي اتكلمنا عنه
+
+571
+00:41:18,160 --> 00:41:21,600
+اللي هو الارتباط بالمانوز والجالاكتروز بالنسبة
+
+572
+00:41:21,600 --> 00:41:27,120
+للفهم اللي هي type 1 و type B هذه الصورة اللي
+
+573
+00:41:27,120 --> 00:41:31,280
+بتوضح اننا قلنا ال .. ال urinary tract infection
+
+574
+00:41:31,280 --> 00:41:35,480
+وقلنا في شكلين من ال UTI اللي هو السيستايتس وكل
+
+575
+00:41:35,480 --> 00:41:39,260
+كتر هو السيستايتس لما دراسنا ال Staphylococcus
+
+576
+00:41:39,260 --> 00:41:42,740
+saprophyticus اللي بتعمل سيستايتس اللي هي ال UTI
+
+577
+00:41:44,600 --> 00:41:48,460
+لكن اللي أول مرة بنحكيله هو البيولينفريتس و كلمة
+
+578
+00:41:48,460 --> 00:41:52,000
+بيولو جاي من كلمة pus معناه الحكي أن ال urine ..
+
+579
+00:41:52,000 --> 00:41:56,940
+ال urine هنلاقيه مليان صديد .. مليان صديد يعني في
+
+580
+00:41:56,940 --> 00:41:59,780
+معنى أخر لما نعمل microscopic analysis of urine
+
+581
+00:42:06,920 --> 00:42:10,760
+واحد لتلاتة، ممكن تلاقي عشرين، ممكن تلاقي مية،
+
+582
+00:42:10,760 --> 00:42:15,600
+ممكن تلاقي full بمعنى ما نستطيع حتى قراءة العالم،
+
+583
+00:42:15,600 --> 00:42:17,660
+ممكن تلاقي مافيه خلايا صدرية
+
+584
+00:42:20,180 --> 00:42:22,960
+it is a leading cause of urinary tract infection
+
+585
+00:42:22,960 --> 00:42:26,860
+which can cause acute cystitis او بيوليو .. بيوليو
+
+586
+00:42:26,860 --> 00:42:30,500
+.. بيولينفراجس اذا ان هي يعني من .. و احنا عملنا
+
+587
+00:42:30,500 --> 00:42:34,900
+حقيقية دراستين في غزة انا شخصيا ساهمت فيهم و انا
+
+588
+00:42:34,900 --> 00:42:39,600
+شاربناهم و في الدراستين بالفعل وجدنا ان ال equal
+
+589
+00:42:39,600 --> 00:42:47,480
+هي number one و يعني من ال .. ال ..طبيعي ان ده جهة
+
+590
+00:42:47,480 --> 00:42:50,380
+number one في كل العالم تقريبا برضه كل الدول اللي
+
+591
+00:42:50,380 --> 00:42:54,480
+هنا فيانه فيانهم طبعا number two اختلف عن بعض
+
+592
+00:42:54,480 --> 00:42:58,860
+الدول لكن number one هو بالفعل الاشريشيا كولان
+
+593
+00:42:58,860 --> 00:43:06,040
+كمان leading cause انا بقول UTI كمان leading cause
+
+594
+00:43:06,040 --> 00:43:11,890
+of neonatal مش اي meningitis neonatal meningitisis
+
+595
+00:43:11,890 --> 00:43:13,910
+leading cause of neonatal meningitis and
+
+596
+00:43:13,910 --> 00:43:17,090
+septicemia with a high mortality rate يبقى نسبة
+
+597
+00:43:17,090 --> 00:43:21,190
+الوفيات في حالة ال neonatal meningitis بتكون عالية
+
+598
+00:43:21,190 --> 00:43:25,170
+despite intensive treatment حتى و رغم انه بنعمل
+
+599
+00:43:25,170 --> 00:43:29,370
+علاج بال antibiotic الا انه الحالات
+
+600
+00:43:33,860 --> 00:43:37,740
+مميزات السلالة اللى بتعمل neonatal meningiosis و
+
+601
+00:43:37,740 --> 00:43:41,900
+سبسيمية انها capsulated و بتحتوي على K1 فاكرين K1
+
+602
+00:43:41,900 --> 00:43:43,880
+او ال K اتفزين اللى هو الكابسولات
+
+603
+00:43:56,380 --> 00:44:02,100
+الـ E.coli capsulated تكون عاملة large colonies و
+
+604
+00:44:02,100 --> 00:44:07,700
+myocardial colonies تشبه مين؟ تشبه ال Eclipsilla
+
+605
+00:44:07,700 --> 00:44:14,920
+لذلك يا بنات انا بتحكيلكم قصة صغيرة توضح كيف احنا
+
+606
+00:44:14,920 --> 00:44:20,820
+مش لازم نعتمد على الشكل فقط لغير في عملية التعرف
+
+607
+00:44:20,820 --> 00:44:25,370
+على الأول ما اشتغلت بالجامعة كان فينا دراسة وكان
+
+608
+00:44:25,370 --> 00:44:30,210
+عندنا عزل .. يعني بنامل دراسة .. بنامل يعني عزل
+
+609
+00:44:30,210 --> 00:44:36,870
+لميكروبات كتيرة من عينات PE فالزملاء
+
+610
+00:44:36,870 --> 00:44:42,910
+اللي كانوا معانا في ال .. في ال .. في القسميعني
+
+611
+00:44:42,910 --> 00:44:46,190
+بيقولوا طب هذه ليش بتاعتبها ال ABI بتاعتبها ال ABI
+
+612
+00:44:46,190 --> 00:44:50,030
+ال environment identification system تبع البكتيريا
+
+613
+00:44:50,030 --> 00:44:52,850
+فبقولهم ده انه انا مش عارف هل هي E.coli و لا E
+
+614
+00:44:52,850 --> 00:44:55,170
+.clepsula و لا E.enterobacter لأ واضح انها E.coli
+
+615
+00:44:55,170 --> 00:44:59,570
+و احنا هذا الرد بهم واضح انها E.coli هي E.clepsula
+
+616
+00:44:59,570 --> 00:45:08,060
+هي E.clepsula فحتى اثبت اللي هم أخدتمنهم تعريفاتهم
+
+617
+00:45:08,060 --> 00:45:10,880
+انت بتقول عنها هذا يعني رقم واحد، ايش بتقول عنها
+
+618
+00:45:10,880 --> 00:45:13,440
+equalize، هذي ايش بتقول عنها eclipse، هذي بتقول
+
+619
+00:45:13,440 --> 00:45:17,720
+عنها كذا كذا كذا، طبعا سجلتهم وعملتلهم ABI، twenty
+
+620
+00:45:17,720 --> 00:45:23,600
+eight، تسعين بالمية راب كانالكلام كان غرب، يبقى
+
+621
+00:45:23,600 --> 00:45:27,640
+الاعتماد على ال morphology لوحده، هذا هو السبب
+
+622
+00:45:27,640 --> 00:45:30,260
+اللي بوصلكم إليها، ان الاعتماد على ال morphology
+
+623
+00:45:30,260 --> 00:45:35,860
+لوحده تمام، مش بيقولوا له إنه سيء، لكن ليس هو
+
+624
+00:45:35,860 --> 00:45:40,010
+الأساس في التعرف، هو بساعدني في التعرف ككـ clue
+
+625
+00:45:40,010 --> 00:45:45,630
+يعني مغزة أو كإشارة لكن ليس هو التعرف الدقيقي اللي
+
+626
+00:45:45,630 --> 00:45:49,010
+بيبقى نتم بيه ال biochemical and physiological
+
+627
+00:45:49,010 --> 00:45:54,230
+testing إما بدونه صعب هاي شوفنا مثلا Klebsiella و
+
+628
+00:45:54,230 --> 00:45:56,390
+احنا فاكر إن Klebsiella طلعت إشريشيا كلها و العكس
+
+629
+00:45:56,390 --> 00:46:02,370
+صحيح قد نجد بعض الأحيان إشريشيا انفكاش تطلع
+
+630
+00:46:02,370 --> 00:46:05,990
+Klebsiella وبالتالي كويس إنه نعمل على الأقل ال
+
+631
+00:46:05,990 --> 00:46:10,760
+minimum biochemical testطيب المرة الجاي ان شاء
+
+632
+00:46:10,760 --> 00:46:14,420
+الله بنبدأ بال .. الميكروبات اللي متعلقة بال E
+
+633
+00:46:14,420 --> 00:46:16,440
+.coli ال تمام infection بال intestinal
+
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/3tfi9d7ClCY_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/3tfi9d7ClCY_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..4220a3bc5892930eeed650812b79f9ca511932ef
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/3tfi9d7ClCY_raw.json
@@ -0,0 +1 @@
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diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/3tfi9d7ClCY_raw.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/3tfi9d7ClCY_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..ddcdc3db6e4734a0a0f744c7197771397275c267
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/3tfi9d7ClCY_raw.srt
@@ -0,0 +1,2552 @@
+1
+00:00:20,500 --> 00:00:23,840
+الله الرحمن الرحيم موضوع جديد حنبدأ فيه ان شاء
+
+2
+00:00:23,840 --> 00:00:28,120
+الله اليوم اللي هو ال gram negative بس الله انا من
+
+3
+00:00:28,120 --> 00:00:30,700
+اول ما بتكلم عن ال gram stain قولتلكوا ال gram
+
+4
+00:00:30,700 --> 00:00:35,900
+stain بيقسم البكتيريا الى اربع مجموعات اما gram
+
+5
+00:00:35,900 --> 00:00:40,880
+negative او gram positive و كل من هماباس الله او
+
+6
+00:00:40,880 --> 00:00:44,100
+كوكساي و اعتقد انه المهادرات السابقة اتعرفنا
+
+7
+00:00:44,100 --> 00:00:48,960
+لنوعين احنا اللي هم ال gram negative كوكساي متمثلة
+
+8
+00:00:48,960 --> 00:00:52,900
+في النيسيريا و gram positive كوكساي متمثلة في
+
+9
+00:00:52,900 --> 00:00:57,080
+الاستفيل الكوكس والاستفت الكوكس والانترو كوكسلكن
+
+10
+00:00:57,080 --> 00:01:00,720
+من أول المحاضرات وانا حضرتها وانت تلاقي أضخم
+
+11
+00:01:00,720 --> 00:01:04,540
+مجموعة على الإطلاق هي ال gram negative بس ال lie
+
+12
+00:01:04,540 --> 00:01:08,320
+اللي احنا بتقدرنا اليوم فتوقع انه برضه يعني يكون
+
+13
+00:01:08,320 --> 00:01:13,820
+في كم كبير من المعلومات متعلقة بهذه المجموعة عندنا
+
+14
+00:01:13,820 --> 00:01:17,320
+كمان مرة gram stain gram negative باللون الزهري و
+
+15
+00:01:17,320 --> 00:01:20,860
+الأحمر و gram positive ال gram negative زي ما
+
+16
+00:01:20,860 --> 00:01:22,840
+انتوا شايفين من قسم ال
+
+17
+00:01:27,750 --> 00:01:30,990
+طبعا احنا هتكلم على ال gram negative بس الله نطلع
+
+18
+00:01:30,990 --> 00:01:35,230
+على ال diagram برضه تاني هذا كتير مهم إذا بتلاحظوا
+
+19
+00:01:35,230 --> 00:01:40,350
+أنه ال .. ال diagram مقسم البكتيريا إلى قسمين اللي
+
+20
+00:01:40,350 --> 00:01:44,170
+هم ال oxidized positive بال verbal color و ال
+
+21
+00:01:44,170 --> 00:01:47,390
+oxidized negative اللي هي colorless سواء كده
+
+22
+00:01:47,390 --> 00:01:51,370
+positive أو negative برضه مجسمينها جسمين بالان على
+
+23
+00:01:51,370 --> 00:01:58,020
+ال glucose fermentation خديني على اللوحةناخد
+
+24
+00:01:58,020 --> 00:02:02,080
+الأسماء الأربعة اللي بيقولوا الأخطر، ناخد أي واحدة
+
+25
+00:02:02,080 --> 00:02:03,900
+منهم، enterobacterias على سبيل المثال، ال
+
+26
+00:02:03,900 --> 00:02:07,740
+enterobacterias هي، نبدأ من تحت و نطلع فوق، هي
+
+27
+00:02:07,740 --> 00:02:11,620
+positive للجلوكوز fermentation، negative
+
+28
+00:02:11,620 --> 00:02:15,680
+للoxidase، ولأن هو الرئيسي gram negative، بس الله،
+
+29
+00:02:15,680 --> 00:02:20,000
+ناخد SodomaurusIt is negative glucose fermentation
+
+30
+00:02:20,000 --> 00:02:24,920
+positive for oxidized gram-negative bacilli إذا
+
+31
+00:02:24,920 --> 00:02:29,520
+نستطعنا من خلال تلت صفات تلت صفات ال gram-negative
+
+32
+00:02:29,520 --> 00:02:33,580
+bacilli و ال oxidized و ال glucose fermentation أن
+
+33
+00:02:33,580 --> 00:02:38,340
+نقسم كل gram-negative bacilli إلى أربع مجموعات
+
+34
+00:02:38,340 --> 00:02:42,510
+مشهورة جدا اللي هي الاسينيتوفاكتاتnegative
+
+35
+00:02:42,510 --> 00:02:46,370
+negative ال enterobacterial negative positive ال
+
+36
+00:02:46,370 --> 00:02:49,690
+vibrio positive positive ال pseudomonas positive
+
+37
+00:02:49,690 --> 00:02:53,050
+negative أعتقد مش صعبين نحفظهم و ضروري نحفظهم
+
+38
+00:02:53,050 --> 00:03:00,030
+التصنيف هذا بالذات .. التصنيف هذا بالذات كتير مهم
+
+39
+00:03:00,030 --> 00:03:03,090
+لأنه practical في الآخر بتجيني ground negative بس
+
+40
+00:03:03,090 --> 00:03:06,750
+ال like بتعرف عليها أي من الأربع بالفحصين اللي أنا
+
+41
+00:03:06,750 --> 00:03:08,050
+ذكرتهم بكل سهولة
+
+42
+00:03:10,720 --> 00:03:16,240
+هنبدأ بالمجموعة الأولى انترو بكترياسي عشان نقول ان
+
+43
+00:03:16,240 --> 00:03:19,640
+بكتيريا X هي انترو بكترياسي لبن تبدأ لخمس صفر
+
+44
+00:03:19,640 --> 00:03:24,980
+وبنحفظهم الآن في المحاضرة سهلين جداit is gram
+
+45
+00:03:24,980 --> 00:03:28,540
+negative بس الله هي واحدة رقم اتنين oxidase
+
+46
+00:03:28,540 --> 00:03:31,780
+negative هذي مهم جدا تخيل ما عاملنا الآن على
+
+47
+00:03:31,780 --> 00:03:35,180
+انتهاء الوقت بكترياسي oxidase negative تلاتة
+
+48
+00:03:35,180 --> 00:03:39,440
+جليكوز في الفرن طب هدولة التلاتة انا شفتهم في ال
+
+49
+00:03:39,440 --> 00:03:44,840
+diagram اللي جابل صح اضافت لهم كمان صفتين الصفة
+
+50
+00:03:44,840 --> 00:03:47,500
+الأولى they are all facultative anaerobic they can
+
+51
+00:03:47,500 --> 00:03:53,430
+grow aerobically and anaerobicرقم خمسة they can
+
+52
+00:03:53,430 --> 00:03:57,610
+reduce nitrate اللي هو ال NO3 to nitrite اللي هو
+
+53
+00:03:57,610 --> 00:04:05,070
+ال NO2 إذا عندي خمس صفات رئيسية إذا توفرت في أي
+
+54
+00:04:05,070 --> 00:04:08,170
+ميكروب بطلق عليه لقب enterobacteris
+
+55
+00:04:10,970 --> 00:04:13,710
+فاكولتات في أنا أوروبا they reduce nitrate to
+
+56
+00:04:13,710 --> 00:04:17,210
+nitrate, they are all glucose fermenter, they are
+
+57
+00:04:17,210 --> 00:04:19,970
+all oxidize negative, they are all gram negative
+
+58
+00:04:19,970 --> 00:04:24,430
+بس اللي لإن هناك صفات أخرى إضافية قد تساعدنا
+
+59
+00:04:24,430 --> 00:04:28,750
+بالتعابف عليها if motile لل enterobacterias إذا
+
+60
+00:04:28,750 --> 00:04:31,270
+كانت متحركة هل معناها الحاجة إن في منها متحرك
+
+61
+00:04:31,270 --> 00:04:35,310
+ومنها غير؟ متحرك فإذا كانت متحركة فهي متحركة
+
+62
+00:04:35,310 --> 00:04:40,120
+بperitricus flagellaيعني توزيع الفلاجلة على الخلية
+
+63
+00:04:40,120 --> 00:04:45,120
+اللي بيكون على كل المناطق رقم اتنين they are non
+
+64
+00:04:45,120 --> 00:04:50,940
+-spore-forming bacteria انا يعني المعلومة ده مهتمش
+
+65
+00:04:50,940 --> 00:04:55,500
+فيها كتير ليش؟ لإن انا أصلا بعرفش في كل ال .. في
+
+66
+00:04:55,500 --> 00:04:58,380
+كل ال medical important bacteria إلا دنتين بيكونوا
+
+67
+00:04:58,380 --> 00:05:02,260
+non-spore-forming وبالتالي ما عداهم خلاص هم non
+
+68
+00:05:02,260 --> 00:05:06,890
+-spore-formingهذا ال diagram عشان يسهل يعني انا
+
+69
+00:05:06,890 --> 00:05:10,730
+احفظه هو نفس المعلومات اللي اللي هو حاسسها يقوله
+
+70
+00:05:10,730 --> 00:05:14,930
+كمان مرة بنطلع نطلع حافظينهم it is gram negative
+
+71
+00:05:14,930 --> 00:05:18,390
+بس الله it is oxidase negative because fermented
+
+72
+00:05:18,390 --> 00:05:22,890
+مش كمان؟ فاكوتيتر فاكوتيتر فانقره مقفل motile non
+
+73
+00:05:22,890 --> 00:05:26,130
+motile motile bipyritric asphalidella وقلنا
+
+74
+00:05:26,130 --> 00:05:29,190
+produces
+
+75
+00:05:29,190 --> 00:05:32,750
+nitrate to nitrite هذه المعلومة بدنا نتكلم عنها
+
+76
+00:05:32,750 --> 00:05:38,520
+شويةال N O 3 N O 3 بتحول الى N O 2
+
+77
+00:05:38,520 --> 00:05:43,220
+بالانتروبكترياسي فقط لا غير لكن هناك بعض أنواع من
+
+78
+00:05:43,220 --> 00:05:47,920
+البكتيريا قد تستطيع تحول ال nitrate الى nitrogen
+
+79
+00:05:47,920 --> 00:05:55,420
+تتحول من N O 3 الى N O 2 ومن ثم الى N 2 بس
+
+80
+00:05:55,420 --> 00:06:02,900
+الانتروبكترياسي فقط N O 2هذه الصفة بتميزنا ان
+
+81
+00:06:02,900 --> 00:06:06,720
+انتقل الوقت ليها ساعات الاخريات اللي قادرين ان هم
+
+82
+00:06:06,720 --> 00:06:14,480
+يحولوها لانتوا طيب نجي لجزء الصعب دايما في ال
+
+83
+00:06:14,480 --> 00:06:18,120
+micro اللي هو ال classification قولنا حوالي 29
+
+84
+00:06:18,120 --> 00:06:22,440
+جنرة يعني احنا زمان بجينا اقول 14 جنرة اربعتاش
+
+85
+00:06:22,440 --> 00:06:27,520
+صاروا ديهم 29يعني طبعا اي مايكروب نكتشفه و بيمتلك
+
+86
+00:06:27,520 --> 00:06:31,040
+الخمس صفات اللي احنا حكينا عنهم بنحطهم في تارو
+
+87
+00:06:31,040 --> 00:06:35,820
+بردر لكن هناك مايكروبات اشهر من مايكروبات بمعنى
+
+88
+00:06:35,820 --> 00:06:40,460
+انه اكتر شيوعا او احنا اتعرفنا عليها منذ فترة
+
+89
+00:06:40,460 --> 00:06:44,640
+طويلة و دول اصلا بشكل جيد امراضها واضحة انهي اللي
+
+90
+00:06:44,640 --> 00:06:48,400
+اتعامل معاها فبالغالب فبالغالب بتكون اشهر من
+
+91
+00:06:48,400 --> 00:06:54,610
+مجموعات اشهر من مجموعات اخرىعلى اللوحة مكتوب تلت
+
+92
+00:06:54,610 --> 00:06:59,130
+ميكروبات أو تلت genesis باللون الأحمر أو اللون
+
+93
+00:06:59,130 --> 00:07:04,330
+البني يعني اللون مميزة Shigella Salmonella Yersin
+
+94
+00:07:04,330 --> 00:07:09,230
+طب احنا يعني حاطيلهم بلون مختلف نعم لكن جابل ما
+
+95
+00:07:09,230 --> 00:07:12,170
+نتكلم عن اللون المختلف خلينا نتكلم عن فكرة معينة
+
+96
+00:07:14,340 --> 00:07:17,280
+سميه enterobacteriasi، بالك و ليش؟ لأنهم
+
+97
+00:07:17,280 --> 00:07:23,180
+enteromaوي، يعني موجودين في القمعة أو بيعملوا
+
+98
+00:07:23,180 --> 00:07:26,560
+أمراض في القمعة، يبقى واحد من اتنين السبب، إما
+
+99
+00:07:26,560 --> 00:07:29,000
+إنهم موجودين naturally في القمعة أو إنهم بيعملوا
+
+100
+00:07:29,000 --> 00:07:32,200
+infection في القمعة، التلاتة اللي ملونين هدولة مش
+
+101
+00:07:32,200 --> 00:07:36,020
+natural، بيعملوا infection في القمعة، زي السنلة و
+
+102
+00:07:36,020 --> 00:07:37,060
+الشجلة و ال
+
+103
+00:07:49,700 --> 00:07:53,940
+يشيريشيا، سيترو بارتر، كليبسيلة و انترو بارتر، يا
+
+104
+00:07:53,940 --> 00:07:59,360
+ريت هدولة انحط جنبهم حرف ال L لكتوز فرمنتر، لكتوز
+
+105
+00:07:59,360 --> 00:08:07,600
+فرمنتر ال F الباقي
+
+106
+00:08:07,600 --> 00:08:12,080
+ايش مانو الباقي؟ اول حاجة انهم لكتوز فرمنتر كلهم،
+
+107
+00:08:12,080 --> 00:08:14,420
+كلهم، سبع كهر الأب اللي هو الأحمر و ليه غير الأحمر
+
+108
+00:08:15,080 --> 00:08:17,700
+لكن البعض .. non-lectors fundamental البعض منهم
+
+109
+00:08:17,700 --> 00:08:23,920
+normal والبعض منهم بقى فور مشي نقرأهم بشكل سريع
+
+110
+00:08:23,920 --> 00:08:28,340
+عشان يصيروا familiar و نبطل نتغلب في حفظهم طبعا
+
+111
+00:08:28,340 --> 00:08:31,160
+انا بتأكد انه صاروا familiar اصلا عندهم من المعنى
+
+112
+00:08:31,160 --> 00:08:36,460
+اللي نظر الشيليشية شيجيلة إدوارد سيلى و الكلمة
+
+113
+00:08:36,460 --> 00:08:39,600
+الكبيرة اللي ممكنش ننفذها بشكل جيد و نقطعها ده بس
+
+114
+00:08:39,600 --> 00:08:42,520
+إدوارد .. إدوارد اسمه معروف إدوارد سيلى
+
+115
+00:08:50,380 --> 00:08:54,780
+السيراتيا عارفينها السيراتيا الحابرة بروتياس
+
+116
+00:08:54,780 --> 00:08:57,840
+بروفيدنشيا
+
+117
+00:08:57,840 --> 00:09:05,820
+مورجانيلا يرسينيا إيروينيا بكتينوباكتيريا بروتياس
+
+118
+00:09:05,820 --> 00:09:12,740
+بروفيدنشيا مورجانيلا PPM PPM parts per million
+
+119
+00:09:16,200 --> 00:09:19,880
+هدول ال PPM هم groups صحيح؟ group بمعنى ان هم ..
+
+120
+00:09:19,880 --> 00:09:22,960
+انا اخلاق انا قلت انه كويس ان نحطهم مع بعض و
+
+121
+00:09:22,960 --> 00:09:26,800
+نسميهم PPM ده ان هم بيسموهم Protease group مجموعة
+
+122
+00:09:26,800 --> 00:09:30,440
+ال Protease اللي بتكون من ال Protease و ال
+
+123
+00:09:30,440 --> 00:09:35,820
+Providentia و ال Morganium بتميزوا
+
+124
+00:09:35,820 --> 00:09:40,940
+بميزة مهمة جدا اسمها Urease producers they produce
+
+125
+00:09:40,940 --> 00:09:45,320
+large amount of Ureasestrong urease producers
+
+126
+00:09:45,320 --> 00:09:52,320
+المصطلح الدقيق strong urease producers طيب عرفنا
+
+127
+00:09:52,320 --> 00:09:56,440
+بعض الأسماء في ال enterobacteriaceae الان نتكلم عن
+
+128
+00:09:56,440 --> 00:09:59,880
+شكل سريع عن ال unclinic structure طبعا الكلام اللي
+
+129
+00:09:59,880 --> 00:10:05,220
+على اللوحة مافيش كلمة فيه جديدة كل الآن بدنا ندخل
+
+130
+00:10:05,220 --> 00:10:09,100
+لمرحلة التكرار مرحلة الاستفادة من المعلومات اللي
+
+131
+00:10:09,100 --> 00:10:11,880
+درسناها لما اتكلمنا عن ال structure او عن ال
+
+132
+00:10:11,880 --> 00:10:12,300
+metabolism
+
+133
+00:10:20,190 --> 00:10:25,430
+عشان نبدأ نتكلم في ال medical microbiome مالذي
+
+134
+00:10:25,430 --> 00:10:27,590
+يميز ال ground negatives ال wall عن ال ground
+
+135
+00:10:27,590 --> 00:10:30,090
+positives ال wall؟ موجود أوتر من بين، مضبوط لو
+
+136
+00:10:30,090 --> 00:10:32,050
+موجود أوتر من بين ومن اللي مضمن اللي هو ال
+
+137
+00:10:32,050 --> 00:10:37,210
+polysaccharide اللي هو ال polysaccharide فاكرينه
+
+138
+00:10:37,210 --> 00:10:42,790
+اللي بيتقيل؟ ال o-sided chain ال o-sided chain و
+
+139
+00:10:42,790 --> 00:10:48,460
+ال o-ال H-antigen طبعا أنا مرتبط الآن وجوده بمين؟
+
+140
+00:10:48,460 --> 00:10:53,120
+بوجود الفلاجلة، لأن هو الفلاجلة الانتجين، احنا لسه
+
+141
+00:10:53,120 --> 00:10:56,560
+قبل الحلقة ذكرنا، كنا F-motile، motile by
+
+142
+00:10:56,560 --> 00:11:01,560
+perifricous flagella، إذا البكتيريا متحركة، ففيها
+
+143
+00:11:01,560 --> 00:11:03,380
+flagella يعني فيها H-antigen
+
+144
+00:11:07,620 --> 00:11:13,280
+الـ H هو common لكل إجراء من الـ negative بس الـ
+
+145
+00:11:13,280 --> 00:11:16,700
+lie قاطبة انترو بكترياسي أو لا انترو بكترياسي
+
+146
+00:11:16,700 --> 00:11:20,120
+common لكن الـ H مورود فقط في تلك اللتي تحتاج
+
+147
+00:11:20,120 --> 00:11:24,520
+عليها اللي هو السمنة كذلك الأول بالنسبة للتالت
+
+148
+00:11:24,520 --> 00:11:29,900
+اللي هو ال capsular antigen أو ال K انتجن و يطلق
+
+149
+00:11:29,900 --> 00:11:32,700
+عليه لقب VI في حدث سمنه لا
+
+150
+00:11:39,210 --> 00:11:41,990
+فكان مسمين الكابسولة الكهربائي كهربائي كهربائي
+
+151
+00:11:41,990 --> 00:11:42,050
+كهربائي كهربائي كهربائي كهربائي كهربائي كهربائي
+
+152
+00:11:42,050 --> 00:11:44,830
+كهربائي كهربائي كهربائي كهربائي كهربائي كهربائي
+
+153
+00:11:44,830 --> 00:11:48,510
+كهربائي كهربائي كهربائي كهربائي كهربائي كهربائي
+
+154
+00:11:48,510 --> 00:11:51,810
+كهربائي
+
+155
+00:11:51,810 --> 00:11:53,250
+كهربائي كهربائي كهربائي كهربائي كهربائي كهربائي
+
+156
+00:11:53,250 --> 00:11:53,950
+كهربائي كهربائي كهربائي كهربائي كهربائي كهربائي
+
+157
+00:11:53,950 --> 00:11:57,350
+كهربائي كهربائي كهربائي كهربائي كهربائي كهربائي
+
+158
+00:11:57,350 --> 00:12:09,110
+كهربائي كهربائي كهربائ
+
+159
+00:12:09,620 --> 00:12:12,080
+يعني ال O أنتجين هي الدول الموجودة للـ O أنتجين
+
+160
+00:12:12,080 --> 00:12:18,340
+فإن
+
+161
+00:12:18,340 --> 00:12:21,840
+هو أنا معاه Antibody و بدي أكشف عن ال O أنتجين في
+
+162
+00:12:21,840 --> 00:12:25,810
+الصورة هذه، ممكن أكشف عنه؟لازم نشيل ال capsule،
+
+163
+00:12:25,810 --> 00:12:30,110
+لازم نشيل ال capsule، إذا وجود capsule will
+
+164
+00:12:30,110 --> 00:12:33,430
+interfere، إيش هال interfere؟ يتفاعل سلبًا،
+
+165
+00:12:33,430 --> 00:12:37,430
+هيمنعنا من الكشف عن ال O-antigen، إذا بدنا .. بدنا
+
+166
+00:12:37,430 --> 00:12:41,430
+نلاقي طريقة نجشر، نشيل فيها ال capsule، نمسك
+
+167
+00:12:41,430 --> 00:12:44,830
+المكشف اللي بطاطا و نمسك ال bacteria اللي بدنا
+
+168
+00:12:44,830 --> 00:12:47,950
+نجشر فيها، ليش رأيكم؟أحنا قادرين نمسكها الباتريا و
+
+169
+00:12:47,950 --> 00:12:54,710
+نشوفها أصلا ففيها عملية تسخين لمدة نصف ساعة على
+
+170
+00:12:54,710 --> 00:12:58,310
+ستين درجة روية بتدمر اللي هو ال capsule بتدمر ال
+
+171
+00:12:58,310 --> 00:13:02,550
+capsule لإنه يبتكشف عن ال O أنتجين فالكلام مرة
+
+172
+00:13:02,550 --> 00:13:07,370
+صورة اللي قدامنا فيها توضيح لثلاث أنواع من
+
+173
+00:13:07,370 --> 00:13:08,130
+الانتجينات
+
+174
+00:13:12,080 --> 00:13:15,160
+الـ Somatic Antigen أو الـ O Antigen وهو مكوّن من
+
+175
+00:13:15,160 --> 00:13:18,960
+الـ Lipid-A و Polysaccharide core و الـ O side
+
+176
+00:13:18,960 --> 00:13:22,300
+chain و الـ O Antigen بالإضافة إلى قلنا مين اللي
+
+177
+00:13:22,300 --> 00:13:28,020
+هو الـ Calcium طيب احنا ذكرنا ثلاث أنتيجينات
+
+178
+00:13:28,020 --> 00:13:33,060
+رئيسية OHK
+
+179
+00:13:33,060 --> 00:13:39,140
+هل هناك أنتيجينات أخرى نعم أقل أهمية لكن مين المهم
+
+180
+00:13:39,140 --> 00:13:43,530
+أكتر جيبنا ان هم التلات هدولةفهن مثلا أعطر من
+
+181
+00:13:43,530 --> 00:13:48,150
+brain proteins كتيرة متنوعة برضه بتشكل كانتجينات
+
+182
+00:13:48,150 --> 00:13:53,750
+داخل جسم الإنسان ال pillar فاكرين ال pillar في
+
+183
+00:13:53,750 --> 00:13:58,270
+محاضرة في محاضرة نيسيريا جونورية فقناها بالخط
+
+184
+00:13:58,270 --> 00:14:02,390
+العريض لإنه كنا هي الأكتر يعني أو أكتر عامل شراسة
+
+185
+00:14:02,390 --> 00:14:08,130
+للبكتيريا واستغلت البكتيريا في تكوين أنواع مختلفة
+
+186
+00:14:08,130 --> 00:14:09,990
+صح؟ من ال antigens
+
+187
+00:14:15,140 --> 00:14:18,700
+بقولها some are encoded by plasmids يعني البعض
+
+188
+00:14:18,700 --> 00:14:22,320
+منها مش محمولة على كروموزومة واننا محمولة على ال
+
+189
+00:14:22,320 --> 00:14:26,080
+plasmid اللى طبعا كانوا كدرسين للمتر بتعرف انها
+
+190
+00:14:26,080 --> 00:14:29,400
+extra chromosomal self-replicating او
+
+191
+00:14:29,400 --> 00:14:37,340
+independently replicated نعنات messenger طيب
+
+192
+00:14:37,340 --> 00:14:41,760
+قبل ما اقفز على النواحى هذه بدنا نحاول ناخد بعض
+
+193
+00:14:44,370 --> 00:14:48,130
+المعلومات اللي قد تفيدنا لماذا ندرس احنا اصلا اللي
+
+194
+00:14:48,130 --> 00:14:50,890
+هو antigens و لايوجد انتجين او capsule انتجين
+
+195
+00:14:50,890 --> 00:14:55,310
+دايما المعلومات المعلومات اي نوع من المعلومات
+
+196
+00:14:55,310 --> 00:14:58,490
+موجودة قدامنا على الطاولة لو كان في عندي واحد
+
+197
+00:14:58,490 --> 00:15:03,630
+فيزيائي واحد كيميائي واحد طبيب واحد مريض واحد من
+
+198
+00:15:03,630 --> 00:15:08,670
+مجال مختلف كل واحد بينظر للمعلومة من وجه نظره
+
+199
+00:15:08,670 --> 00:15:12,830
+الخاصة يعني لو كنا ما فاقت الانتجينات هنقول من وجه
+
+200
+00:15:12,830 --> 00:15:19,110
+نظر مينالمريض ولا البكتيريا ولا التشخيص اللى هو ال
+
+201
+00:15:19,110 --> 00:15:22,050
+medical microbiology ولا الطبيب كل واحد اللى هو
+
+202
+00:15:22,050 --> 00:15:26,870
+أهداف مختلفة من المعلومة المتعلقة بمين بالانتجنة
+
+203
+00:15:26,870 --> 00:15:32,450
+احنا نبدأ بحالنا microbiologist ايش بنستفيد من
+
+204
+00:15:32,450 --> 00:15:37,510
+الانتجنة تاقول ايه كلها؟ ايش قلت عنها قبل شوية
+
+205
+00:15:37,510 --> 00:15:42,890
+انت؟ مورودة بشكل natural فلقا معاهاطب لو قلتلكوا
+
+206
+00:15:42,890 --> 00:15:45,670
+انا ايكوا والله one five seven eight seven .. اي
+
+207
+00:15:45,670 --> 00:15:49,270
+كم مرة حكيتها هي اليوم اللي كانت؟ كتير صح؟ سابقين؟
+
+208
+00:15:49,270 --> 00:15:52,130
+تلقى معاها برضه؟ طيب لو عزلنا .. اذا انا و احد
+
+209
+00:15:52,130 --> 00:15:55,690
+معاه اسهل و عزلنا من ال stool تبقى اي شي ليه شك
+
+210
+00:15:55,690 --> 00:16:00,030
+والله .. ممكن تكون نوم؟ ممكن تكون نوم؟ ممكن تكون
+
+211
+00:16:00,030 --> 00:16:02,990
+نوم؟ ممكن تكون نوم؟ ممكن تكون نوم؟ ممكن تكون نوم؟
+
+212
+00:16:02,990 --> 00:16:03,390
+ممكن تكون نوم؟ ممكن تكون نوم؟ ممكن تكون نوم؟ ممكن
+
+213
+00:16:03,390 --> 00:16:03,390
+تكون نوم؟ ممكن تكون نوم؟ ممكن تكون نوم؟ ممكن تكون
+
+214
+00:16:03,390 --> 00:16:03,450
+نوم؟ ممكن تكون نوم؟ ممكن تكون نوم؟ ممكن تكون نوم؟
+
+215
+00:16:03,450 --> 00:16:04,230
+ممكن تكون نوم
+
+216
+00:16:09,080 --> 00:16:12,060
+الـ Strain او السلالة more specifically اتعرفنا
+
+217
+00:16:12,060 --> 00:16:15,860
+عليها الان المرحلة اللي نقلناها للطبيب ايش فيها من
+
+218
+00:16:15,860 --> 00:16:21,700
+هو ال body pathogen انها مش normal flora بيس يعني
+
+219
+00:16:21,700 --> 00:16:26,580
+انا استفدت في المقتبر ان اتعرفت بشكل ادق على بنا
+
+220
+00:16:26,580 --> 00:16:31,720
+ال species E coli and strain E coli O157H7 نحن
+
+221
+00:16:31,720 --> 00:16:37,530
+مثال تانيفي أواخر التسعينية للقرن الماضي صار لنا
+
+222
+00:16:37,530 --> 00:16:43,810
+وضع كلها في غزة يعني كلها .. كلها .. الان لو انا
+
+223
+00:16:43,810 --> 00:16:46,630
+بتعمل investigation لل source كلكم بتعرفوا ان كلها
+
+224
+00:16:46,630 --> 00:16:49,650
+تنتقل عن طريق ال water pollution يعني ماء ملوة
+
+225
+00:16:49,650 --> 00:16:51,870
+المياه .. مش كلها مصدر
+
+226
+00:16:56,530 --> 00:17:01,010
+من بلديات، وحتى البلدية الواحدة في المدينة الواحدة
+
+227
+00:17:01,010 --> 00:17:08,870
+قد تزود السكان بمياه ليست مصدر واحد، يعني هما
+
+228
+00:17:08,870 --> 00:17:10,930
+كانوا مصادر مختلفة ومتنوعة، يعني حتى المنطقة اللي
+
+229
+00:17:10,930 --> 00:17:16,810
+كانت غزة كبيرة جالها المنطقة مثلا المخيم أو كده،
+
+230
+00:17:16,810 --> 00:17:20,940
+ففي النهاية أنا بدي أعرف مصدر الماء الملوثلا يعني
+
+231
+00:17:20,940 --> 00:17:26,220
+ان انا عزلت Vibrio من ما يجهي نفس ال Vibrio لعمل
+
+232
+00:17:26,220 --> 00:17:33,380
+الوباءلازم اعرف الانتيجينات للبكتيريا اللى معزولة
+
+233
+00:17:33,380 --> 00:17:38,100
+من الوضع من المرضى يعنى و اعرف الانتيجينات اللى
+
+234
+00:17:38,100 --> 00:17:42,800
+معزولة من العينات البيئية بيها من ضمنها انها اذا
+
+235
+00:17:42,800 --> 00:17:48,140
+توافقت عينات كانتيجينات اللى معزولة من البيئة مع
+
+236
+00:17:48,140 --> 00:17:52,480
+تلك اللى توزنت من المرضى فبيقول هذا most likely is
+
+237
+00:17:52,480 --> 00:17:54,320
+a source of this disease
+
+238
+00:17:58,180 --> 00:18:02,780
+أو نوحي للناس بإنهم يستخدموا او بنكلوا احنا بنقول
+
+239
+00:18:02,780 --> 00:18:05,800
+بإجراءات مثلا بنكلوا هذا الماء اللي بنحط عليه
+
+240
+00:18:05,800 --> 00:18:09,360
+الكلوم يعني بناخد إجراء بين عالمين على مالماتنا من
+
+241
+00:18:09,360 --> 00:18:13,280
+الانتجار هذا الشيء نسميه هنا source tracking إيش
+
+242
+00:18:13,280 --> 00:18:17,200
+أنا source tracking؟ track تتبقى source المصدر
+
+243
+00:18:17,200 --> 00:18:20,920
+يبقى تبقى المصدر في المستشفى .. في المستشفى صاروا
+
+244
+00:18:20,920 --> 00:18:24,720
+بقى في أحد الأقسام و الله افترض الحضارة على أحد
+
+245
+00:18:24,720 --> 00:18:28,440
+الأسافر اللي بيحدثبعض الأحيان، في ال nursery،
+
+246
+00:18:28,440 --> 00:18:31,340
+حبارة اللي هم بيحطوا فيها الأطفال الحديثين الولادة
+
+247
+00:18:31,340 --> 00:18:33,780
+اللي هم غالبا الخدد اللي يقولوا أنهم premature
+
+248
+00:18:33,780 --> 00:18:39,720
+babies، أطفال اللي أعمارهم حمير أقل من 9 شهور أو
+
+249
+00:18:39,720 --> 00:18:44,020
+اللي يقولوا أنهم عندهم إشكالية، فصار النوبة
+
+250
+00:18:44,020 --> 00:18:48,560
+ببكتيريا X، الآن بكتيريا X زي Staphylococcus مثلا
+
+251
+00:18:48,560 --> 00:18:53,160
+أو السيرافيةجينا بنعرف مصدر تبعها خدنا من العاملين
+
+252
+00:18:53,160 --> 00:18:56,180
+لاجينا سيراتيا و سيراتيا و سيراتيا بس ماجياش عندك
+
+253
+00:18:56,180 --> 00:18:59,360
+انت سيراتيا انت مش متهانة خالص لكن ماجياش تهام
+
+254
+00:18:59,360 --> 00:19:03,520
+واحد اتنين تلاتة ماعندهم سيراتيا تفهم الا الا
+
+255
+00:19:03,520 --> 00:19:07,040
+اتأكدنا ان السيراتيا اللى عزلناها من المريض هي نفس
+
+256
+00:19:07,040 --> 00:19:10,100
+السيراتيا اللى عزلناها ولما بقيتم الا عن طريق
+
+257
+00:19:10,100 --> 00:19:13,960
+غالبا الانتجينات نعرف الانتجينات تبعت البقية ايه
+
+258
+00:19:13,960 --> 00:19:17,460
+ده source tracking source tracking is very
+
+259
+00:19:17,460 --> 00:19:21,310
+importantميزنا في المثال الأول ميزنا بين pathogen
+
+260
+00:19:21,310 --> 00:19:25,150
+و non pathogen لما قولنا إيه شريش يا كولاي السؤال
+
+261
+00:19:25,150 --> 00:19:28,010
+على طول كان so what ماهي natural لك لما قولنا إيه
+
+262
+00:19:28,010 --> 00:19:32,450
+كولاي O157H7 يبقى ده أنا صافيا معرفة بين أن هذا
+
+263
+00:19:32,450 --> 00:19:38,270
+السؤال هي pathogen إذا بنا نفهم إنه احنا بالنسبة
+
+264
+00:19:38,270 --> 00:19:43,170
+إنه في ال microbiology نتعرف على ال .. ال .. ال ..
+
+265
+00:19:47,020 --> 00:19:55,040
+أدق لهوية الكائن لأسباب متنوعة مختلفة
+
+266
+00:19:55,040 --> 00:20:01,240
+الانترن بактерياسي ممكن احنا جوناها يعني محترمة في
+
+267
+00:20:01,240 --> 00:20:03,660
+ال intestinal tract او مواطنة محترمة في ال
+
+268
+00:20:03,660 --> 00:20:07,260
+intestinal tract موجودة بشكل طبيعي بالعكس يعني مش
+
+269
+00:20:07,260 --> 00:20:09,740
+بس محترمة لها دور كويس في ال intestinal tract
+
+270
+00:20:14,560 --> 00:20:19,040
+لاتعوي و لا انها normal microbiota ستقوم بعمل
+
+271
+00:20:19,040 --> 00:20:23,660
+infection و أهم واحدة انزل تحت اول واحدة من تحت
+
+272
+00:20:23,660 --> 00:20:27,540
+you urinary tract infection لو تعرف ان erysia coli
+
+273
+00:20:27,540 --> 00:20:30,540
+هي number one cause of urinary tract infection
+
+274
+00:20:33,510 --> 00:20:37,130
+لقيها number one في شغل أكتر من شغل بس من ضمنها it
+
+275
+00:20:37,130 --> 00:20:41,690
+may cause septicemia it may cause pneumonia it may
+
+276
+00:20:41,690 --> 00:20:45,530
+cause meningitis ولا الصحيح اللوحى دى مابدى نفهم
+
+277
+00:20:45,530 --> 00:20:48,730
+منها ان بس هدول هم اللي بيسبوا أوراق ولا مابدى
+
+278
+00:20:48,730 --> 00:20:50,950
+نفهم ان بس هدول هم الأوراق اللي بيسبوا هم هدول
+
+279
+00:20:50,950 --> 00:20:54,910
+المجموعة بس هتعطينا نموذة بقى انه outside the
+
+280
+00:20:54,910 --> 00:20:58,760
+intestinal tract they may cause disease ومنهاإن هو
+
+281
+00:20:58,760 --> 00:21:01,600
+خطير قد يصل إلى اللي هقولنا ال meningitis و
+
+282
+00:21:01,600 --> 00:21:03,880
+الcysticemia و ال pneumonia و ال urinary tract
+
+283
+00:21:03,880 --> 00:21:06,640
+infection طب حتى ال urinary tract infection في
+
+284
+00:21:06,640 --> 00:21:10,120
+منها ال bayonifritis و ال salgilia is a very
+
+285
+00:21:10,120 --> 00:21:13,280
+dangerous one وبالتالي بدنا نكون عارفين إن ال UTI
+
+286
+00:21:13,280 --> 00:21:15,680
+مش حاجة سهلة طيب
+
+287
+00:21:18,110 --> 00:21:21,890
+الانتلا بكتيريا سنة قولت انه نجسمها جسمين، جسم كان
+
+288
+00:21:21,890 --> 00:21:25,910
+reside normally، يعني هو normal flora، و جسم اخر
+
+289
+00:21:25,910 --> 00:21:28,710
+كان cause infection في ال gas في الانتلاك، من
+
+290
+00:21:28,710 --> 00:21:31,830
+الانتلاك .. طبعا كل الانتلاك اللي كانوا موالين
+
+291
+00:21:31,830 --> 00:21:34,590
+باللون البرني فاكرينهم؟ رفارامين
+
+292
+00:21:37,900 --> 00:21:42,880
+مقنع خوفينا هناك لإنه احنا يعني موزعين بين نسميها
+
+293
+00:21:42,880 --> 00:21:46,720
+normal flora ولا البعض .. البعض ثلاث منها اللي
+
+294
+00:21:46,720 --> 00:21:51,320
+بتسبب infection نسميها pathogen فهي الآن صرنا بحق
+
+295
+00:21:51,320 --> 00:21:55,320
+إنه لازم نجوانها pathogen في ال intestinal tract
+
+296
+00:21:55,320 --> 00:21:58,660
+Salmonella, Shigella, Biersinia, Entero, Coelum
+
+297
+00:22:01,160 --> 00:22:03,860
+بنعرف ان ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+298
+00:22:03,860 --> 00:22:03,900
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+299
+00:22:03,900 --> 00:22:03,960
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+300
+00:22:03,960 --> 00:22:04,620
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+301
+00:22:04,620 --> 00:22:05,120
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+302
+00:22:05,120 --> 00:22:05,800
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+303
+00:22:05,800 --> 00:22:06,380
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+304
+00:22:06,380 --> 00:22:11,360
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+305
+00:22:11,360 --> 00:22:15,940
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+306
+00:22:15,940 --> 00:22:16,260
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+307
+00:22:16,260 --> 00:22:16,260
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+308
+00:22:16,260 --> 00:22:16,260
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+309
+00:22:16,260 --> 00:22:16,340
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+310
+00:22:16,340 --> 00:22:16,340
+.. ال
+
+311
+00:22:21,210 --> 00:22:25,490
+وجود أي سيروتاعو .. وجود أي سيروتاعو السلمونيلا في
+
+312
+00:22:25,490 --> 00:22:28,390
+الغداء يجعل هذا الغداء غير صاحب الاستهلاك الأناني
+
+313
+00:22:28,390 --> 00:22:36,370
+ما نحاكي على الإطلاق السلمونيلا pathogenic وجودها
+
+314
+00:22:36,370 --> 00:22:41,810
+.. وجودها يجعل الغداء والشراب والطعام أيا كان غير
+
+315
+00:22:41,810 --> 00:22:47,070
+صاحب الاستهلاك الأناني السلمونيلا العقب اللي
+
+316
+00:22:47,070 --> 00:22:49,930
+هحكيلكوا هو أنه نبضة صغيرة جابنا صلها عن
+
+317
+00:22:49,930 --> 00:22:56,130
+السلمونيلاوعن الشجلة، هم لما بنقول سلمانوله أصلا
+
+318
+00:22:56,130 --> 00:23:01,460
+بنقول الشجلة، سلمانوله and شجلةboth has very
+
+319
+00:23:01,460 --> 00:23:04,380
+similar characteristics او يعني very common
+
+320
+00:23:04,380 --> 00:23:07,760
+characteristics منها انهم gram-negative بسلعي،
+
+321
+00:23:07,760 --> 00:23:09,900
+they are enterobacteriasi وكلمة enterobacteriasi
+
+322
+00:23:09,900 --> 00:23:12,800
+يعني كل الصفات المشتركة موجودة، they are non
+
+323
+00:23:12,800 --> 00:23:16,420
+-lactose fermenter، non-lactose fermenter، لكن
+
+324
+00:23:16,420 --> 00:23:19,520
+بتبعتنا بسبب pointestinal tract infection، لكن
+
+325
+00:23:19,520 --> 00:23:23,360
+برضه هناك أوجه اختلاف كبيرة، كبيرة، كبيرة، كبيرة،
+
+326
+00:23:23,360 --> 00:23:26,960
+متعاطف ال diseaseالشيكيلة has never been isolated
+
+327
+00:23:26,960 --> 00:23:30,580
+from sites other than the intestinal tract بمعنى
+
+328
+00:23:30,580 --> 00:23:34,420
+أنه لم .. ما بتسيبش غير ال intestinal tract بس
+
+329
+00:23:34,420 --> 00:23:37,340
+المنلة ممكن أجلبها من one infection، من unary
+
+330
+00:23:37,340 --> 00:23:40,980
+tract، من ال blood، من CSF، من أماكن مختلفة في جسم
+
+331
+00:23:40,980 --> 00:23:45,800
+الإنسان، أمام؟ هذا واحد رقم اتنين يعني بمعنى أخر
+
+332
+00:23:45,800 --> 00:23:50,420
+أنه الشيكيلة بتسيب one disease اللي هواللي هو
+
+333
+00:23:50,420 --> 00:23:53,000
+intestinal tract infection بس Salmonella بتسبب
+
+334
+00:23:53,000 --> 00:23:56,140
+بالاضافة إلى intestinal tract infection أمور أخرى
+
+335
+00:23:56,140 --> 00:24:03,360
+كثيرة الشجلة ماعرفنهاش غير في الإنسان ماتعرفش يعني
+
+336
+00:24:03,360 --> 00:24:08,760
+مخلوق آخر يصاب بالشجلة غير الإنسان يعني نظريا لو
+
+337
+00:24:08,760 --> 00:24:12,640
+دورنا على كل البشر اللي مصابين بالشجلة لو علجناهم
+
+338
+00:24:12,640 --> 00:24:16,740
+سياخد على مين؟ على الشجلة نفسه على الدزيسة على
+
+339
+00:24:16,740 --> 00:24:23,110
+الشجلة نظريابينما السلمونيلا وجدت من العوائل
+
+340
+00:24:23,110 --> 00:24:29,470
+نسميها واحدة reservoir، مخازن، من العوائل ما يعني
+
+341
+00:24:29,470 --> 00:24:36,270
+لا تتخيله، شفت الحردون، السحلية، التمساح، السلاحف،
+
+342
+00:24:36,270 --> 00:24:41,510
+الدواجن، البيض اللي احنا ممكن ناكله ممكن يكون
+
+343
+00:24:41,510 --> 00:24:45,770
+جواته سلمونيلاالمؤامرة بينها وبين الدواجن كبيرة يا
+
+344
+00:24:45,770 --> 00:24:50,250
+بنات ووصلت لإن الدواجن تسمح للسالمونيلا تروح ال
+
+345
+00:24:50,250 --> 00:24:55,030
+ovary وتحقن البيض بخليئة أو خليتان من السالمونيلا
+
+346
+00:24:55,030 --> 00:25:01,190
+وبالتالي انتوا عارفين البيض الآن بيدخل كمنتج في
+
+347
+00:25:01,190 --> 00:25:07,490
+شغلات كتيرة صح ولا لأ طب الدواجن جنة وفيها
+
+348
+00:25:07,490 --> 00:25:14,580
+سالمونيلا الدواجن الآنبنطبخها احنا و الطبخ تبعنا
+
+349
+00:25:14,580 --> 00:25:19,140
+مش على هوا البارد بنسخنها بظبط، بنسخنها اما
+
+350
+00:25:19,140 --> 00:25:22,520
+بالشاوري او بالقلي او بالسلق، بالغالية، بالغالية
+
+351
+00:25:22,520 --> 00:25:26,080
+الميه وهذه غالبا الحرارة بتكون كافية لقتل
+
+352
+00:25:26,080 --> 00:25:30,920
+السلمونيلا إذا الدجاجة نفسها اللي أكلناها حتى لو
+
+353
+00:25:30,920 --> 00:25:34,000
+كان فيها سلمونيلا، غالبا we are protected إلا إذا
+
+354
+00:25:34,000 --> 00:25:37,140
+كان undercooked، undercooked يعني مقلق لم تتعرف
+
+355
+00:25:37,140 --> 00:25:39,780
+لدرجة حرارة كويسة، وانا اتكلم في الشاوري وفي
+
+356
+00:25:39,780 --> 00:25:40,860
+القلي، غالبا
+
+357
+00:25:44,080 --> 00:25:47,720
+الصفحة بتكون عالية لكن في ال center بتكون .. عشانك
+
+358
+00:25:47,720 --> 00:25:51,280
+لو بقى بتفتح حرطة لو لسه .. لسه بنزل دم مش .. يعني
+
+359
+00:25:51,280 --> 00:25:55,580
+مش مستوية ال .. طب وين المشكلة؟ المشكلة في المطبخ
+
+360
+00:25:55,580 --> 00:26:00,820
+واحدة من القطة في اللحم تمام بيحدث ما يسمى cross
+
+361
+00:26:00,820 --> 00:26:04,680
+contamination السكين اللي استخدمناها و الخشب
+
+362
+00:26:04,680 --> 00:26:08,000
+التقطيع ال chopping board اللي استخدمناها هذه الأن
+
+363
+00:26:08,000 --> 00:26:11,750
+أصبحت عليها سالمونةاحنا أخدنا الجولة و أحطناها في
+
+364
+00:26:11,750 --> 00:26:15,290
+الغابة، ما مات سبن اللي هناك بس إنها لها .. لها
+
+365
+00:26:15,290 --> 00:26:18,950
+سكينة و لها chopping board ما matter، غسلت السكينة
+
+366
+00:26:18,950 --> 00:26:22,090
+لسه ليك خفيف أو ال chopping board ما غسلتيها كويس
+
+367
+00:26:22,090 --> 00:26:27,890
+و جيت و عملت بقدونسولا شو .. يعني أي نوع من الأغدى
+
+368
+00:26:27,890 --> 00:26:31,990
+اللي .. اللي لا .. لا يطهر، يعني سلطات، تمام؟
+
+369
+00:26:31,990 --> 00:26:36,770
+فأخدنا السلمونيلا .. أخدنا السلمونيلا من ال .. ال
+
+370
+00:26:36,770 --> 00:26:39,910
+.. ال chiming board أو من السكين وحطناها في غذاء
+
+371
+00:26:39,910 --> 00:26:43,390
+لن يتعرض لحرارة عالية بتاعي سينقذ لنا السلمونيلا،
+
+372
+00:26:43,390 --> 00:26:46,570
+هذا بيسميها حالة cross contamination وهذه الأخطر
+
+373
+00:26:46,570 --> 00:26:53,270
+الصحيح في السلمونيلا، هذا كان اليوم محاضرة تثقيفية
+
+374
+00:26:53,270 --> 00:26:53,850
+في المطبخ
+
+375
+00:26:56,610 --> 00:27:00,030
+بس هذا .. هذا الكلام .. هذا الكلام بيحتم علينا انه
+
+376
+00:27:00,030 --> 00:27:04,770
+دايما فصل الخضروات و ال .. ال .. عن اللحوم، يعني
+
+377
+00:27:04,770 --> 00:27:07,430
+اي شيء متعمد فيه في ال .. في المطبخ، خضروات علينا
+
+378
+00:27:07,430 --> 00:27:11,950
+نفصلها عن اللحوم و الأسماك و ما شابه ده يعني،
+
+379
+00:27:11,950 --> 00:27:15,670
+فإفريشيا، كولاي، سلمونيلا، شيجيلا، يرسينيا، انترو،
+
+380
+00:27:15,670 --> 00:27:16,050
+كل شيء
+
+381
+00:27:19,320 --> 00:27:22,660
+الان ال enterobacteriaceae بدنا ننظر إليها من
+
+382
+00:27:22,660 --> 00:27:27,080
+منظور كمان مرة إن حتى في ال community في المجتمع
+
+383
+00:27:27,080 --> 00:27:32,780
+ممكن نروح المستشفى احنا بأمراض اكتسبناها من
+
+384
+00:27:32,780 --> 00:27:37,480
+المجتمع بتكون نتيجة عن ال enterobacteriaceae مثلا
+
+385
+00:27:37,480 --> 00:27:42,560
+Declepsia pneumonia Declepsia pneumonia بتسبب من
+
+386
+00:27:42,560 --> 00:27:50,430
+اسمها pneumonia وطبعا بتسبب pneumonia وإليهامؤهل
+
+387
+00:27:50,430 --> 00:27:53,170
+لل .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+388
+00:27:53,170 --> 00:27:53,190
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+389
+00:27:53,190 --> 00:27:53,390
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+390
+00:27:53,390 --> 00:27:54,250
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+391
+00:27:54,250 --> 00:27:55,790
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+392
+00:27:55,790 --> 00:27:55,810
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+393
+00:27:55,810 --> 00:27:57,910
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+394
+00:27:57,910 --> 00:27:58,990
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+395
+00:27:58,990 --> 00:28:00,790
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+396
+00:28:00,790 --> 00:28:00,790
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+397
+00:28:00,790 --> 00:28:01,730
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+398
+00:28:01,730 --> 00:28:02,830
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+399
+00:28:02,830 --> 00:28:08,990
+ال .. ال .. ال .. ال .. ال .. ال ..
+
+400
+00:28:08,990 --> 00:28:17,210
+ال .. ال ..
+
+401
+00:28:20,240 --> 00:28:25,940
+Polysaccharide Capsule الورداني ترد انفكشن واللي
+
+402
+00:28:25,940 --> 00:28:28,860
+بتنتج عن غرب على الفيكال Contamination could be
+
+403
+00:28:28,860 --> 00:28:35,600
+caused by E.coli E.coli
+
+404
+00:28:35,600 --> 00:28:39,240
+Clipsilla Enterovacter and Proteus البروتياس إلها
+
+405
+00:28:39,240 --> 00:28:42,560
+ميزة على الواقع فاكرين قبل شوية احنا قولنا بروتياس
+
+406
+00:28:42,560 --> 00:28:47,460
+جروب PPM بروتياس مورجانيلا و البروفيدنش They are
+
+407
+00:28:47,460 --> 00:28:52,540
+all strong UGAsstrong urea is يعني they can
+
+408
+00:28:52,540 --> 00:28:57,360
+convert urea to ammonia and CO2 ammonia is
+
+409
+00:28:57,360 --> 00:29:02,180
+alkaline يبقى ال urine .. ال urine عند الناس
+
+410
+00:29:02,180 --> 00:29:07,540
+المصابين بالبروتيا هيكون ال pH تبعهم alkaline لما
+
+411
+00:29:07,540 --> 00:29:09,840
+تدرسوا ال urine analysis انتوا ان شاء الله هتعرفوا
+
+412
+00:29:09,840 --> 00:29:13,740
+ان ال pH تبع ال urine بيقاس بنجيس ال pH تبع ال
+
+413
+00:29:13,740 --> 00:29:15,920
+urine بيفرق لما يكون acidic على neutral لما يكون
+
+414
+00:29:15,920 --> 00:29:19,660
+ايه؟ alkaline بال strip بال strip صحيح
+
+415
+00:29:27,060 --> 00:29:33,500
+الان ال ph تبع ال urine is essential for some
+
+416
+00:29:33,500 --> 00:29:34,960
+salts
+
+417
+00:29:37,510 --> 00:29:42,130
+اللي احنا عشان بسميهم احنا الأملاح to precipitate
+
+418
+00:29:42,130 --> 00:29:45,030
+في البعض منهم بيصير ال precipitation على high pH
+
+419
+00:29:45,030 --> 00:29:48,050
+والبعض الاخر بيصير ال precipitation على low pH
+
+420
+00:29:48,050 --> 00:29:51,730
+فبالتالي لو كان الأملاح دي موجودة و ال pH alkaline
+
+421
+00:29:51,730 --> 00:29:55,130
+عمال الترسيب بتكون واحدة وبالتالي تكوين حصوات أصلا
+
+422
+00:29:55,130 --> 00:29:59,310
+ككانوكيا بتكون أيضا يعني فنقدر نقول إن ال Protase
+
+423
+00:29:59,310 --> 00:30:04,130
+قد تساهم في تكوين بعض الواعي من ال stones في ال
+
+424
+00:30:04,130 --> 00:30:05,910
+urinary tract
+
+425
+00:30:08,950 --> 00:30:13,050
+ولا مرة ولا بأي حال من الأحوال اللوحة اللي قدامنا
+
+426
+00:30:13,050 --> 00:30:18,330
+هي فقط التدليل على ان ال antibacterial بتسبب
+
+427
+00:30:18,330 --> 00:30:21,710
+community acquired infections اقولكوا من أشهر
+
+428
+00:30:21,710 --> 00:30:24,090
+community acquired infections wound infections
+
+429
+00:30:25,070 --> 00:30:29,090
+تمام؟ بتعرفوا أطفال ان احنا بحبوا الفصل الشتاء و
+
+430
+00:30:29,090 --> 00:30:33,370
+بكيفه لما تكون الشرارة لسه يعني مليئة لميه لكن
+
+431
+00:30:33,370 --> 00:30:37,350
+للأسف ال sewage system انا في قطاع درس زي شفته
+
+432
+00:30:37,350 --> 00:30:41,810
+يعني في شوية أنطار بسيطة يعني صار في بعض المناطق
+
+433
+00:30:41,810 --> 00:30:45,830
+فيها يعني عدم قدرة على الأنظمة ال sewage من
+
+434
+00:30:45,830 --> 00:30:49,730
+الستوار و لتاني صار فينا طوفان في بعض المناطق فلما
+
+435
+00:30:49,730 --> 00:30:54,500
+الأطفال تمشيفي ال .. ال .. هذه المياه اللى الآن
+
+436
+00:30:54,500 --> 00:30:57,500
+اختلطت بال sewage اللى من ضمن ال sewage stool ..
+
+437
+00:30:57,500 --> 00:31:01,080
+stool يعني انتروه بكترياسي من ضمن هاتريشيا كولار
+
+438
+00:31:01,080 --> 00:31:04,280
+لو عنده اي scratch فى ال .. فى ال .. فى المناطق
+
+439
+00:31:04,280 --> 00:31:06,880
+اللى اتعرضت للماء ممكن يصيب عنده wound infection
+
+440
+00:31:06,880 --> 00:31:10,100
+wound infection اذا لان بيبقى عالم ممكن يصيب عنده
+
+441
+00:31:10,100 --> 00:31:13,530
+septicemiaإذا لم يُعرفوا أنهم لديهم meningitis
+
+442
+00:31:13,530 --> 00:31:17,690
+فنعم يعني بمعنى واخر أن ال .. ال .. ال
+
+443
+00:31:17,690 --> 00:31:21,970
+enterovaginase مصدر مهم للعدوى في ال community كما
+
+444
+00:31:21,970 --> 00:31:27,610
+هي مصدر مهم للعدوى في المستشفى نبدأ بالمايكروب
+
+445
+00:31:27,610 --> 00:31:33,790
+الأول اللي هو الشريشيا كولا نبدأ
+
+446
+00:31:33,790 --> 00:31:36,450
+ب .. بعض ال .. ال ..
+
+447
+00:31:42,130 --> 00:31:45,390
+غير انكوا تستحضروا الاحترام والتقديم للبكتيريا،
+
+448
+00:31:45,390 --> 00:31:48,210
+انكوا تذكروا ان ما بتتكلم عن .. ما بتتكلم عن
+
+449
+00:31:48,210 --> 00:31:53,550
+سلالة، بتتكلم عن سلالات، قد واحدة منهم تحتوي على
+
+450
+00:31:53,550 --> 00:31:58,750
+واحد او اكثر من هذه الأراسل، تمام؟ وقد تحتوي على
+
+451
+00:31:58,750 --> 00:32:03,810
+اتنين او تلاتة، اربع، حسب النوع اللي هو السلالة،
+
+452
+00:32:03,810 --> 00:32:09,990
+بتكونوا يعرفوا ان كل ال enterobacteriumبدون ال
+
+453
+00:32:09,990 --> 00:32:15,670
+lipopolysaccharide مابتسوش حاجة بمعنى و الكلام تم
+
+454
+00:32:15,670 --> 00:32:19,150
+تجذيبه انه في بعض الميكروبات أزيل منها ال
+
+455
+00:32:19,150 --> 00:32:23,650
+lipopolysaccharide فقدت قدرتها على ال pathogenesis
+
+456
+00:32:23,650 --> 00:32:28,170
+فال lipopolysaccharide هذا أمر مهم جدا اللي احنا
+
+457
+00:32:28,170 --> 00:32:32,610
+أخدنا من ضمنه أخدنا منه مين فقد ال endotoxin في
+
+458
+00:32:32,610 --> 00:32:36,610
+هذه الحالة كبيرة بالنسبة لك لكن البكتيريا
+
+459
+00:32:37,700 --> 00:32:40,800
+طب .. طب بدي أخلق على النص شويه في المحاضرة و
+
+460
+00:32:40,800 --> 00:32:45,720
+انحاول انه .. برضه نثبت بعض ال .. او مش نثبت ..
+
+461
+00:32:45,720 --> 00:32:50,660
+نحاول انه يعني نقرب بعض الأفكار اللي كانت غريضة
+
+462
+00:32:50,660 --> 00:32:56,300
+لزمان الشيشة كلها طول عمرها مسالمة، natural في ال
+
+463
+00:32:56,300 --> 00:33:00,900
+intestinal flora فجأة لجناها منها سرلات بتنتج أو
+
+464
+00:33:00,900 --> 00:33:04,400
+تمال ديارية و لما دورنا على ال toxin تبعها على
+
+465
+00:33:04,400 --> 00:33:09,520
+جناب بيشبه الشيجل، فسموه shigalو مرة رأى تكسين
+
+466
+00:33:09,520 --> 00:33:14,000
+مشهور كتير اسمه LT تكسين بيشبه الكوليرات، لحظة
+
+467
+00:33:14,000 --> 00:33:19,100
+بيشبه الكوليرات، بيشبه الشجلة، ففاكرين ان الفكرة
+
+468
+00:33:19,100 --> 00:33:24,300
+تبعت انه gene transferإنه J Transfer إنه ممكن
+
+469
+00:33:24,300 --> 00:33:27,720
+بكتيريا هو J Transfer لما بيقولوا conjugation
+
+470
+00:33:27,720 --> 00:33:31,460
+غالبا بيقولوا Species لأ غير Species مع Species
+
+471
+00:33:31,460 --> 00:33:35,640
+بنفس ال Species يعني يعني E coli مع E coli بس الآن
+
+472
+00:33:35,640 --> 00:33:40,020
+واضح من ال .. إنه في .. إنه في إمكانية لانتقال من
+
+473
+00:33:40,020 --> 00:33:43,320
+genus ل genus من genus ل genus يعني مثلا ممكن من
+
+474
+00:33:43,320 --> 00:33:47,300
+Shigella انتقل لمين؟ ل Escherichia coli من
+
+475
+00:33:47,300 --> 00:33:54,370
+Salmonella ل .. او من Vibrio ل Escherichia coliوإن
+
+476
+00:33:54,370 --> 00:34:00,070
+في ممكن مكان يلتقي فيه ميكروفات ومناسب و تتبادل مع
+
+477
+00:34:00,070 --> 00:34:03,450
+بعض الانتجنات، سويش systems، انظمة المجال ال
+
+478
+00:34:03,450 --> 00:34:06,190
+intestinal tract، تمام؟ لو واحد أصيب بسلامه من
+
+479
+00:34:06,190 --> 00:34:10,130
+الله، ما هو ال E.coli موجودة، إمكانية إنه يصير فيه
+
+480
+00:34:10,130 --> 00:34:13,590
+تبادل، الموقع بيكون عالي، قد تنجح وقد تبني، لذلك
+
+481
+00:34:13,590 --> 00:34:19,250
+كل فترة وفترة بنلاقي سلولات جديدة من ال E.coliيعني
+
+482
+00:34:19,250 --> 00:34:23,930
+جامل ممكن تلت سنوات طلعت سولالة فى ألمانيا وصار
+
+483
+00:34:23,930 --> 00:34:27,850
+حتى يعني زعل من أسبانيا تهم .. ألمانيا تهمت
+
+484
+00:34:27,850 --> 00:34:30,730
+أسبانيا أنه من ال .. طماطم المستورد .. او من
+
+485
+00:34:30,730 --> 00:34:33,690
+الخيار المستورد حتى المصريين أطلقوا عليها إعلاميا
+
+486
+00:34:33,690 --> 00:34:36,910
+بكتير الخيار، اه المصريين بيحبوا ي .. لأ و في
+
+487
+00:34:36,910 --> 00:34:42,210
+الآخر طلعت ملخ حراقة بال .. بالأسبانيا، أوروبا
+
+488
+00:34:44,500 --> 00:34:49,540
+أوروبا مرتع لظهور صورة جديدة من اللي هو الاشريشيا
+
+489
+00:34:49,540 --> 00:34:51,920
+كولاي، احنا بالنسبة لإنه يمكن يكون مولود هناك
+
+490
+00:34:51,920 --> 00:34:56,240
+لإنها واخش يعني، بس هم عندهم أدوات ال diagnosis
+
+491
+00:34:56,240 --> 00:35:00,390
+دقيقة وبتالي بيكون في عندهم تتبعيبقى انا اللي
+
+492
+00:35:00,390 --> 00:35:04,150
+بتواصله من الكلام الأخير هذا انه احتمالية انتقال
+
+493
+00:35:04,150 --> 00:35:06,830
+جينات من microbe ل microbe وردة وهذا احد اهم
+
+494
+00:35:06,830 --> 00:35:09,850
+الأسباب اللي دايما بتقولنا انه في ال microbiology
+
+495
+00:35:09,850 --> 00:35:15,230
+مافيش عنها ثبات في انها تغيير باستمرار فمثلا عنه
+
+496
+00:35:15,230 --> 00:35:20,210
+toxins زي shiga type toxin وentelo aggregative ST
+
+497
+00:35:20,210 --> 00:35:23,210
+like toxin ST يعني stable toxin like
+
+498
+00:35:27,240 --> 00:35:33,340
+Hemolysins, endotoxins, type 3 secretion system هو
+
+499
+00:35:33,340 --> 00:35:40,020
+عبارة عن نظام حقن بوصف اسمه molecular syringes
+
+500
+00:35:45,600 --> 00:35:51,220
+فهو أبارعاً سيرنج سيرنج أو مايش بالسيرنج لكن صغيرة
+
+501
+00:35:51,220 --> 00:35:54,560
+جداً ده يسموها molecular syringes البكتيريا
+
+502
+00:35:54,560 --> 00:36:00,920
+بتستخدمها لحق مراكبات إما في بكتيريا أخرى أو في
+
+503
+00:36:00,920 --> 00:36:07,560
+host cell المراكبة هذه بتاع بقى تخيل معايا ال E
+
+504
+00:36:07,560 --> 00:36:10,840
+.coli موجودة في ال intestinal tract على enterocyte
+
+505
+00:36:10,840 --> 00:36:15,570
+على enterocyte خلية من خلال ال intestinal tractال
+
+506
+00:36:15,570 --> 00:36:19,250
+enterocyte مش معنية بلا تعمل phagocytosis لل
+
+507
+00:36:19,250 --> 00:36:25,250
+إيشريشا كولايا مش معنية فالإيشريشا كولايا بتحقق ان
+
+508
+00:36:25,250 --> 00:36:28,910
+فيها مادة بتقولها .. بتوشوشها المادة بتقولها زي
+
+509
+00:36:28,910 --> 00:36:33,410
+ابليسيا بتقولها يعني انت حاجك ناجس طب ما انت ما
+
+510
+00:36:33,410 --> 00:36:36,010
+شاء الله تمتلك مقومات ال macro phage ومبتملك
+
+511
+00:36:36,010 --> 00:36:40,780
+مقومات ال phagocyte كلين انا غذاء جيدتخيلوا
+
+512
+00:36:40,780 --> 00:36:45,640
+بكتيريا الجلة الخالية كليني ايش معناها؟ eat me ايش
+
+513
+00:36:45,640 --> 00:36:49,920
+معناها؟ انها بدها تدخل الى الداخل، بدها تدخل الى
+
+514
+00:36:49,920 --> 00:36:54,880
+الداخل، و لو عارفة حالها هتموت في الداخل، كان
+
+515
+00:36:54,880 --> 00:36:59,480
+مبادرها كليني، كان المبادر ت exist intracellularly
+
+516
+00:37:00,510 --> 00:37:05,330
+فالان ايش اللي بيصير ان السيرنج بتحكم الخلية اللي
+
+517
+00:37:05,330 --> 00:37:09,930
+هو السلب بمواد تعمل حد يسموها polymerization of
+
+518
+00:37:09,930 --> 00:37:13,010
+أكتين بتعمل أكتين تخير معايا الخلية بهذا الشكل
+
+519
+00:37:13,010 --> 00:37:18,950
+اللي مخليها واجفة ومنتفخة ومخت شكلها هو ال
+
+520
+00:37:18,950 --> 00:37:22,650
+cytoskeleton عارفين مما يتكون ال cytoskeleton؟ من
+
+521
+00:37:22,650 --> 00:37:26,870
+الأكتين polymer فبتروح تعمل polymerization للأكتين
+
+522
+00:37:26,870 --> 00:37:30,300
+where الخلية الموجودة هي الموجودة هنا الخليةتخيلوا
+
+523
+00:37:30,300 --> 00:37:34,820
+البوطن هذه الصغيرة، تمام؟ فبيروح بالأكتين بيرفع
+
+524
+00:37:34,820 --> 00:37:38,140
+السجف منها و بيرفع السجف منها و بيصير عنده كذا
+
+525
+00:37:38,140 --> 00:37:42,640
+أقدام كاملة و البكتيريا بتصير في النصر بيبلعها من
+
+526
+00:37:42,640 --> 00:37:48,620
+داخل إلى داخل زي الفاجوزوم و هي في الفاجوزوم الآن،
+
+527
+00:37:48,620 --> 00:37:52,020
+هي موجودة هنا في الفاجوزوم لما البكتيريا تقوم في
+
+528
+00:37:52,020 --> 00:37:56,440
+الفاجوزوم، إيش أول خطوة بيتيجي الآن؟ ال ISO zone
+
+529
+00:37:56,440 --> 00:38:00,830
+عشان يصير الفاجوزوم ال ISO zoneعلى طول بتطلع الـ
+
+530
+00:38:00,830 --> 00:38:04,210
+syringe تبعها و بتحقن ال membrane و بتقول
+
+531
+00:38:04,210 --> 00:38:09,910
+الفاجوزوم false alarm يعني جرس انظر خاطر انت خليك
+
+532
+00:38:09,910 --> 00:38:14,590
+مطرحك مافيش عنا مشاكل خليك مكانك طبعا لما
+
+533
+00:38:14,590 --> 00:38:18,330
+الفاجوزوم مايرتبطش .. يا عفوا الفاجوزوم مايرتبطش
+
+534
+00:38:18,330 --> 00:38:18,990
+في الفاجوزوم
+
+535
+00:38:22,190 --> 00:38:25,770
+بعد ذلك يبدأ
+
+536
+00:38:25,770 --> 00:38:30,010
+البكتيريا تنقسم داخل الخلية انقسام البكتيريا داخل
+
+537
+00:38:30,010 --> 00:38:36,850
+الخلية خد بالك له معاني كثيرة في جهاز المناعم بعيد
+
+538
+00:38:36,850 --> 00:38:37,150
+عن
+
+539
+00:38:41,140 --> 00:38:45,980
+بعيد عن ال antibodies إذا في antibodies لأن جوا
+
+540
+00:38:45,980 --> 00:38:48,320
+الخلية ال antibiotics بتدخل جوا الخلية مش هدخل جوا
+
+541
+00:38:48,320 --> 00:38:51,700
+الخلية صح ولا لأ تركيز ال antibiotics لو في
+
+542
+00:38:51,700 --> 00:38:55,680
+antibiotics في الخلايا أكيد هيكون أقل من تركيزه في
+
+543
+00:38:55,680 --> 00:39:01,940
+الدمفهي محصرة نوعا ما داخل الخلال اللي نبعتنا و
+
+544
+00:39:01,940 --> 00:39:07,180
+تتكاثر و تستخدم الخلية كمادة غدائية لها في التكاثر
+
+545
+00:39:07,180 --> 00:39:12,880
+إذا ال type 3 secretion system نظام رائع جدا لخداء
+
+546
+00:39:12,880 --> 00:39:21,380
+الخلالة و من ثم حق الملكبات يعني لتطوية ال host
+
+547
+00:39:21,380 --> 00:39:23,840
+لصلاة
+
+548
+00:39:25,600 --> 00:39:29,460
+ال .. ال additions و طلع منها اكتر من نوع من ال ..
+
+549
+00:39:29,460 --> 00:39:33,820
+من ال adhesins موجودين في ال ecotype و ان في حوالي
+
+550
+00:39:33,820 --> 00:39:39,860
+واحد و عشرين different types او الا كيف بدنا نفسر
+
+551
+00:39:39,860 --> 00:39:43,690
+.. كيف بدنا نفسر urinary tract infectionالبكتيريا
+
+552
+00:39:43,690 --> 00:39:49,350
+دخلت من ال urethra، من مكان منخفض، و بيطلع فوق عكس
+
+553
+00:39:49,350 --> 00:39:52,310
+التيار، التيار مين؟ ال urethra اللي نزل تحت، صعبة
+
+554
+00:39:52,310 --> 00:39:55,390
+بقى، تطلع و تصل لكيليا، و تعمل bionephritis، كيف
+
+555
+00:39:55,390 --> 00:39:59,370
+بينافسر الكلام؟ بوجود نقدرة هائلة على ال
+
+556
+00:39:59,370 --> 00:40:01,550
+attachment من قبل اللي هو البكتيريا اللي اسمها
+
+557
+00:40:01,550 --> 00:40:05,180
+ischiavaالـ virulence factors that protect the
+
+558
+00:40:05,180 --> 00:40:07,980
+bacteria from most defenses زي الكبسول وال iron
+
+559
+00:40:07,980 --> 00:40:10,920
+capturing ability يعني عندها مقدرة على أنها تبرض
+
+560
+00:40:10,920 --> 00:40:14,760
+ترتبط بمين بالحديد ولا بيساعد على المنافسة معاها
+
+561
+00:40:14,760 --> 00:40:18,300
+اللي هو اللي هو أصلا ايضا في تبتلك outer membrane
+
+562
+00:40:18,300 --> 00:40:21,120
+proteins إذا ان في مشموعة كبيرة من ال virulence
+
+563
+00:40:21,120 --> 00:40:25,080
+factors موجودة في الإشريشيا كلها البعض بقرنة وبعض
+
+564
+00:40:25,080 --> 00:40:28,880
+آخر حد هتكره بالتفصيلة هتكر كل نوع من الإشريشيا
+
+565
+00:40:34,010 --> 00:40:38,550
+بيجاوبنا على أسئلة كثيرة قد تساعدنا في الفهم بعضنا
+
+566
+00:40:38,550 --> 00:40:42,230
+البعض
+
+567
+00:40:42,230 --> 00:40:44,910
+انا براحة قبل ان احضر قطار انا حاطط فيديو على
+
+568
+00:40:44,910 --> 00:40:51,320
+الفيسبوك بمضحك الليستيرياالليستيريا كيف تخترق
+
+569
+00:40:51,320 --> 00:40:57,060
+الخلية إلى خلية أخرى بدل إنها تطلع برا تمام؟ وتعمل
+
+570
+00:40:57,060 --> 00:41:00,360
+infection لخلية تانية؟ ده بيعمل حاجة اسمها actin
+
+571
+00:41:00,360 --> 00:41:04,180
+rockets اللي هي صواريخ باكتين وبتضرب حاجة بتروح
+
+572
+00:41:04,180 --> 00:41:08,860
+ليها على الخلية التانية يارتبوا الحسابة تبعنا في
+
+573
+00:41:08,860 --> 00:41:11,440
+ال essential medical micro وفي الفيسبوك لإنه أنا
+
+574
+00:41:11,440 --> 00:41:15,100
+كل فترة فترة بضيف يعني بعض الأشياء الجاية المناسبة
+
+575
+00:41:15,480 --> 00:41:18,160
+هذه الصورة اللي كتبتها قبل هيك اللي اتكلمنا عنه
+
+576
+00:41:18,160 --> 00:41:21,600
+اللي هو الارتباط بالمانوز والجالاكتروز بالنسبة
+
+577
+00:41:21,600 --> 00:41:27,120
+للفهم اللي هي type 1 و type B هذه الصورة اللي
+
+578
+00:41:27,120 --> 00:41:31,280
+بتوضح اننا قلنا ال .. ال urinary tract infection
+
+579
+00:41:31,280 --> 00:41:35,480
+وقلنا في شكلين من ال UTI اللي هو السيستايتس وكل
+
+580
+00:41:35,480 --> 00:41:39,260
+كتر هو السيستايتس لما دراسنا ال Staphylococcus
+
+581
+00:41:39,260 --> 00:41:42,740
+saprophyticus اللي بتعمل سيستايتس اللي هي ال UTI
+
+582
+00:41:44,600 --> 00:41:48,460
+لكن اللي أول مرة بنحكيله هو البيولينفريتس و كلمة
+
+583
+00:41:48,460 --> 00:41:52,000
+بيولو جاي من كلمة pus معناه الحكي أن ال urine ..
+
+584
+00:41:52,000 --> 00:41:56,940
+ال urine هنلاقيه مليان صديد .. مليان صديد يعني في
+
+585
+00:41:56,940 --> 00:41:59,780
+معنى أخر لما نعمل microscopic analysis of urine
+
+586
+00:42:06,920 --> 00:42:10,760
+واحد لتلاتة، ممكن تلاقي عشرين، ممكن تلاقي مية،
+
+587
+00:42:10,760 --> 00:42:15,600
+ممكن تلاقي full بمعنى ما نستطيع حتى قراءة العالم،
+
+588
+00:42:15,600 --> 00:42:17,660
+ممكن تلاقي مافيه خلايا صدرية
+
+589
+00:42:20,180 --> 00:42:22,960
+it is a leading cause of urinary tract infection
+
+590
+00:42:22,960 --> 00:42:26,860
+which can cause acute cystitis او بيوليو .. بيوليو
+
+591
+00:42:26,860 --> 00:42:30,500
+.. بيولينفراجس اذا ان هي يعني من .. و احنا عملنا
+
+592
+00:42:30,500 --> 00:42:34,900
+حقيقية دراستين في غزة انا شخصيا ساهمت فيهم و انا
+
+593
+00:42:34,900 --> 00:42:39,600
+شاربناهم و في الدراستين بالفعل وجدنا ان ال equal
+
+594
+00:42:39,600 --> 00:42:47,480
+هي number one و يعني من ال .. ال ..طبيعي ان ده جهة
+
+595
+00:42:47,480 --> 00:42:50,380
+number one في كل العالم تقريبا برضه كل الدول اللي
+
+596
+00:42:50,380 --> 00:42:54,480
+هنا فيانه فيانهم طبعا number two اختلف عن بعض
+
+597
+00:42:54,480 --> 00:42:58,860
+الدول لكن number one هو بالفعل الاشريشيا كولان
+
+598
+00:42:58,860 --> 00:43:06,040
+كمان leading cause انا بقول UTI كمان leading cause
+
+599
+00:43:06,040 --> 00:43:11,890
+of neonatal مش اي meningitis neonatal meningitisis
+
+600
+00:43:11,890 --> 00:43:13,910
+leading cause of neonatal meningitis and
+
+601
+00:43:13,910 --> 00:43:17,090
+septicemia with a high mortality rate يبقى نسبة
+
+602
+00:43:17,090 --> 00:43:21,190
+الوفيات في حالة ال neonatal meningitis بتكون عالية
+
+603
+00:43:21,190 --> 00:43:25,170
+despite intensive treatment حتى و رغم انه بنعمل
+
+604
+00:43:25,170 --> 00:43:29,370
+علاج بال antibiotic الا انه الحالات
+
+605
+00:43:33,860 --> 00:43:37,740
+مميزات السلالة اللى بتعمل neonatal meningiosis و
+
+606
+00:43:37,740 --> 00:43:41,900
+سبسيمية انها capsulated و بتحتوي على K1 فاكرين K1
+
+607
+00:43:41,900 --> 00:43:43,880
+او ال K اتفزين اللى هو الكابسولات
+
+608
+00:43:56,380 --> 00:44:02,100
+الـ E.coli capsulated تكون عاملة large colonies و
+
+609
+00:44:02,100 --> 00:44:07,700
+myocardial colonies تشبه مين؟ تشبه ال Eclipsilla
+
+610
+00:44:07,700 --> 00:44:14,920
+لذلك يا بنات انا بتحكيلكم قصة صغيرة توضح كيف احنا
+
+611
+00:44:14,920 --> 00:44:20,820
+مش لازم نعتمد على الشكل فقط لغير في عملية التعرف
+
+612
+00:44:20,820 --> 00:44:25,370
+على الأول ما اشتغلت بالجامعة كان فينا دراسة وكان
+
+613
+00:44:25,370 --> 00:44:30,210
+عندنا عزل .. يعني بنامل دراسة .. بنامل يعني عزل
+
+614
+00:44:30,210 --> 00:44:36,870
+لميكروبات كتيرة من عينات PE فالزملاء
+
+615
+00:44:36,870 --> 00:44:42,910
+اللي كانوا معانا في ال .. في ال .. في القسميعني
+
+616
+00:44:42,910 --> 00:44:46,190
+بيقولوا طب هذه ليش بتاعتبها ال ABI بتاعتبها ال ABI
+
+617
+00:44:46,190 --> 00:44:50,030
+ال environment identification system تبع البكتيريا
+
+618
+00:44:50,030 --> 00:44:52,850
+فبقولهم ده انه انا مش عارف هل هي E.coli و لا E
+
+619
+00:44:52,850 --> 00:44:55,170
+.clepsula و لا E.enterobacter لأ واضح انها E.coli
+
+620
+00:44:55,170 --> 00:44:59,570
+و احنا هذا الرد بهم واضح انها E.coli هي E.clepsula
+
+621
+00:44:59,570 --> 00:45:08,060
+هي E.clepsula فحتى اثبت اللي هم أخدتمنهم تعريفاتهم
+
+622
+00:45:08,060 --> 00:45:10,880
+انت بتقول عنها هذا يعني رقم واحد، ايش بتقول عنها
+
+623
+00:45:10,880 --> 00:45:13,440
+equalize، هذي ايش بتقول عنها eclipse، هذي بتقول
+
+624
+00:45:13,440 --> 00:45:17,720
+عنها كذا كذا كذا، طبعا سجلتهم وعملتلهم ABI، twenty
+
+625
+00:45:17,720 --> 00:45:23,600
+eight، تسعين بالمية راب كانالكلام كان غرب، يبقى
+
+626
+00:45:23,600 --> 00:45:27,640
+الاعتماد على ال morphology لوحده، هذا هو السبب
+
+627
+00:45:27,640 --> 00:45:30,260
+اللي بوصلكم إليها، ان الاعتماد على ال morphology
+
+628
+00:45:30,260 --> 00:45:35,860
+لوحده تمام، مش بيقولوا له إنه سيء، لكن ليس هو
+
+629
+00:45:35,860 --> 00:45:40,010
+الأساس في التعرف، هو بساعدني في التعرف ككـ clue
+
+630
+00:45:40,010 --> 00:45:45,630
+يعني مغزة أو كإشارة لكن ليس هو التعرف الدقيقي اللي
+
+631
+00:45:45,630 --> 00:45:49,010
+بيبقى نتم بيه ال biochemical and physiological
+
+632
+00:45:49,010 --> 00:45:54,230
+testing إما بدونه صعب هاي شوفنا مثلا Klebsiella و
+
+633
+00:45:54,230 --> 00:45:56,390
+احنا فاكر إن Klebsiella طلعت إشريشيا كلها و العكس
+
+634
+00:45:56,390 --> 00:46:02,370
+صحيح قد نجد بعض الأحيان إشريشيا انفكاش تطلع
+
+635
+00:46:02,370 --> 00:46:05,990
+Klebsiella وبالتالي كويس إنه نعمل على الأقل ال
+
+636
+00:46:05,990 --> 00:46:10,760
+minimum biochemical testطيب المرة الجاي ان شاء
+
+637
+00:46:10,760 --> 00:46:14,420
+الله بنبدأ بال .. الميكروبات اللي متعلقة بال E
+
+638
+00:46:14,420 --> 00:46:16,440
+.coli ال تمام infection بال intestinal
+
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/5rnHqFi4ziI.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/5rnHqFi4ziI.srt
new file mode 100644
index 0000000000000000000000000000000000000000..813c452dd3c7450fc10fa81c72b1acbc9b26eca3
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/5rnHqFi4ziI.srt
@@ -0,0 +1,2435 @@
+1
+00:00:20,670 --> 00:00:22,550
+بسم الله الرحمن الرحيم أنا هأتكلم اليوم عن موضوع جديد
+
+2
+00:00:22,550 --> 00:00:26,330
+إن شاء الله بعنوان bacterial vaccines هو العنوان
+
+3
+00:00:26,330 --> 00:00:30,850
+جديد لكن أنا متأكد المعلومات اللي هنشوفها ضمن هذا
+
+4
+00:00:30,850 --> 00:00:36,110
+ال chapter هتلاقي أن جزء كبير منها تم دراسته كنا
+
+5
+00:00:36,110 --> 00:00:39,190
+بنعرف على سبيل المثال أن من الميكروبات اللي
+
+6
+00:00:39,190 --> 00:00:42,770
+درسناهم Salmonella كنت prevented by الـ vaccine
+
+7
+00:00:42,770 --> 00:00:47,860
+Shigella كنت prevented by الـ vaccine كثير من
+
+8
+00:00:47,860 --> 00:00:50,300
+الأمراض اللي اضطررتنا إليها قلنا vaccine
+
+9
+00:00:50,300 --> 00:00:53,400
+preventable disease قلت لي هل أنا بعرفها في بعض
+
+10
+00:00:53,400 --> 00:00:57,820
+الأمراض هذه اليوم هنحاول أن نعمل categorization
+
+11
+00:00:57,820 --> 00:01:02,480
+عملية تصنيف للـ vaccine و تعريف قلنا الـ process
+
+12
+00:01:02,480 --> 00:01:06,710
+immunization اللي هم نقولها دعاها بالتطعيم أو 
+
+13
+00:01:06,710 --> 00:01:10,450
+التوقيع could be active و could be passive يعني
+
+14
+00:01:10,450 --> 00:01:14,970
+ممكن نعطي انتيجينات وممكن نعطي antibodies إذا 
+
+15
+00:01:14,970 --> 00:01:18,930
+أعطينا انتيجينات الجسم بيكون antibodies وأنا بسميه
+
+16
+00:01:18,930 --> 00:01:23,590
+active immunization إذا أعطينا الـ universe جهزة
+
+17
+00:01:23,590 --> 00:01:27,870
+من برة بيسميها passive immunization والحالتين
+
+18
+00:01:27,870 --> 00:01:32,110
+مطموضين أن نعمل active ونعمل passive ناخد أول
+
+19
+00:01:32,110 --> 00:01:36,130
+مثال أكتر immunity is induced by vaccines prepared
+
+20
+00:01:36,130 --> 00:01:39,070
+from bacteria or their products يبقى الـ vaccines
+
+21
+00:01:39,070 --> 00:01:44,930
+في اللوحة هذه بتقول كلمتين مهمين إما الـ vaccine هي
+
+22
+00:01:44,930 --> 00:01:48,710
+عبارة عن إما البكتيريا نفسها أو منتجاتها
+
+23
+00:01:48,710 --> 00:01:53,810
+والبكتيريا إما killed or attenuated يعني إما ميتة
+
+24
+00:01:53,810 --> 00:02:00,320
+أو مضاعفة أو منتجاتها إيش هنا منتجاتها؟ ممكن
+
+25
+00:02:00,320 --> 00:02:05,100
+proteins ممكن fragments من الـ proteins أو من
+
+26
+00:02:05,100 --> 00:02:09,300
+الخلية إذا في الآخر مش الخلية كلها وده أجزاء من
+
+27
+00:02:09,300 --> 00:02:13,020
+الخلية الـ passive زي ما حكينا it is preformed
+
+28
+00:02:13,020 --> 00:02:17,460
+antibodies يعني أجسام مضادة مجهزة في الخارج قاطعة
+
+29
+00:02:17,460 --> 00:02:22,040
+للمريض لحمايته من مرض معين أو يبعده من مرض معين
+
+30
+00:02:23,030 --> 00:02:26,950
+رقم تلاتة ليلة غريبة active and passive at the
+
+31
+00:02:26,950 --> 00:02:31,610
+same time بنعطي vaccine وبنعطي immunoglobulin وفي
+
+32
+00:02:31,610 --> 00:02:35,490
+مثالين صحيحين في الطب مشهورين جداً في هذا المجال
+
+33
+00:02:35,490 --> 00:02:41,670
+نبدأ فيهم أنت منهم تلعب تيتانوس؟ تيتانوس والـ
+
+34
+00:02:41,670 --> 00:02:44,710
+rabies الـ rabies يمكن يكون أسهل للشرح خلنا نشرحها
+
+35
+00:02:44,710 --> 00:02:49,120
+الـ rabies الـ rabies هو يبقى عبارة عن دا الكلاب اللي
+
+36
+00:02:49,120 --> 00:02:53,980
+لو عضّ .. كلمة عضّ يعني .. لأنه طريقة الانتقال عن
+
+37
+00:02:53,980 --> 00:02:57,560
+طريق الكلاب اتضالت والغالب اللي هم صورة في بعض
+
+38
+00:02:57,560 --> 00:03:01,020
+البلدان لأن الكلاب تم تطعيمها وخلصنا من الكلاب
+
+39
+00:03:01,020 --> 00:03:04,040
+اتضالة فصار الانتقال عن طريق أشياء أخرى زي
+
+40
+00:03:04,040 --> 00:03:07,380
+الخفافيش على سبيل المثال وعن طريق السناجب ..
+
+41
+00:03:07,380 --> 00:03:09,720
+بتعرف السناجب الحلوين هدول؟ هدول اللي ممكن
+
+42
+00:03:09,720 --> 00:03:13,380
+ينقلونها الـ .. في حيوانات كثيرة ممكن تنقل اللي هو
+
+43
+00:03:13,380 --> 00:03:18,520
+الـ rabies الـ animal بيسموه rabid animal، rabid
+
+44
+00:03:18,520 --> 00:03:22,900
+animal يعني حيوان عنده مرض اللي هو rabies، rabid
+
+45
+00:03:22,900 --> 00:03:28,480
+animal الـ rabid animal لو عضّ إنسان، فالآن في
+
+46
+00:03:28,480 --> 00:03:33,200
+احتمالية أن هذا الشخص صار .. لأن هو exposed، صار
+
+47
+00:03:33,200 --> 00:03:37,680
+إيش أنّه exposed؟ تعرض، فالحاجة الأحسن اللي احنا
+
+48
+00:03:37,680 --> 00:03:41,380
+نعطيه، نعطيه vaccine ونعطيه antibodies، هو تعرض
+
+49
+00:03:41,380 --> 00:03:46,830
+already مظبوط نعطيه antibodies لكن لأنّه .. لأنّه الـ
+
+50
+00:03:46,830 --> 00:03:51,470
+virus اللي هو رحلته من الجلد إلى الـ central nervous
+
+51
+00:03:51,470 --> 00:03:55,610
+system بتاخد أيام هذه الفترة الزمنية .. هذه الفترة
+
+52
+00:03:55,610 --> 00:04:02,190
+الزمنية كافية أن أنا أشتغل بالطريقتين يبقى أحكم
+
+53
+00:04:02,190 --> 00:04:07,070
+المكان المصاب بـ immunoglobulins immunoglobulins هل
+
+54
+00:04:07,070 --> 00:04:10,930
+بيقبل neutralization؟ لأ اللي هو الـ anti .. الـ
+
+55
+00:04:10,930 --> 00:04:17,190
+vaccine .. الـ virus .. الـ virus نفسه وعلى الجهة
+
+56
+00:04:17,190 --> 00:04:21,930
+التانية مثلاً اللي هو العضة في رجله اليمين تمام؟
+
+57
+00:04:21,930 --> 00:04:25,680
+بروح على أيديه اليمين وبعطيه vaccine ليش بعضهم مش
+
+58
+00:04:25,680 --> 00:04:28,800
+في نفس المكان؟ عشان الـ vaccine إيش هو؟ Antigen
+
+59
+00:04:28,800 --> 00:04:31,580
+وهذا اللي حطيناها بالأول Antibody، لو اتقابل
+
+60
+00:04:31,580 --> 00:04:34,100
+Antibody مع الـ Antigen هي Value Neutralization، صح
+
+61
+00:04:34,100 --> 00:04:37,580
+ولا لأ؟ فبعطي الـ vaccine في جهة وبعطي الـ
+
+62
+00:04:37,580 --> 00:04:40,520
+immunoglobulin في جهة تانية كمان، ليش عطينا
+
+63
+00:04:40,520 --> 00:04:44,480
+vaccine في هذه الحالة؟ لأن في فترة زمنية طويلة ما
+
+64
+00:04:44,480 --> 00:04:48,680
+بين الـ site of entry لإنه يصل لـ center of impulse
+
+65
+00:04:48,680 --> 00:04:52,960
+system ففي فرصة أن الاسم يكون أسام مضادة إلى هاي
+
+66
+00:04:52,960 --> 00:04:55,280
+مثلها الـ active passive immunization في نفس اللي
+
+67
+00:04:55,280 --> 00:04:58,160
+هو الوقت لكن في غير بالأمر إيه هتعطيه منه بيبولين
+
+68
+00:04:58,160 --> 00:05:03,500
+إيه هتعطيه فاكسينه ده على حساب الـ Active
+
+69
+00:05:03,500 --> 00:05:06,780
+Immunity ممكن تقسم الفاكسين إلى تلات أنواع أو تلات
+
+70
+00:05:06,780 --> 00:05:09,720
+أصحاب من الفاكسين الصندوق الأول الـ capsular
+
+71
+00:05:09,720 --> 00:05:12,580
+polysaccharide فستجدوا حالكوا بتاعه فوق كويس
+
+72
+00:05:12,580 --> 00:05:16,000
+Inactivated Protein Toxins اللي هي الـ Toxites
+
+73
+00:05:16,000 --> 00:05:20,540
+وبتاعه فوق وضبط كويس تلاتة killed bacteria or live
+
+74
+00:05:20,540 --> 00:05:23,540
+attenuated bacteria كلمة live يعني
+
+75
+00:05:29,820 --> 00:05:34,280
+بالنسبة للمعالجات المطلوبة، لكن البكتيريا اتعرضت
+
+76
+00:05:34,280 --> 00:05:38,340
+له معالجات جعلتها غير قادرة على إحداث طعام
+
+77
+00:05:38,340 --> 00:05:41,620
+ال genesis وبالتالي هي attenuated قري لزمي الـ
+
+78
+00:05:41,620 --> 00:05:44,820
+infection، لكن غير قادرة على إحداث الـ disease
+
+79
+00:05:44,820 --> 00:05:50,810
+وبالتالي المريض بيحتك بمجموعة من البكتيريا أو
+
+80
+00:05:50,810 --> 00:05:55,490
+بيقتلها معناه حاكيش أنّه عنده experience، كلمة
+
+81
+00:05:55,490 --> 00:05:58,710
+experience يعني عنده memory وعنده antibodies لو
+
+82
+00:05:58,710 --> 00:06:01,790
+البكتيريا يخلق مرة أخرى بطريقة natural، بيقدر
+
+83
+00:06:01,790 --> 00:06:04,570
+يقاوم، إذن captured by polysaccharide، inactivated
+
+84
+00:06:04,570 --> 00:06:08,790
+proteins تكسوك، تلقى a killed bacteria or live
+
+85
+00:06:08,790 --> 00:06:16,060
+attenuated bacteria نجوم الـ meningitis فاكرينهم؟
+
+86
+00:06:16,060 --> 00:06:19,620
+هم نفسهم النجوم اللي يلهم الكابسول صح ولا لا؟
+
+87
+00:06:19,620 --> 00:06:23,540
+وبالتالي كون الكابسول is the major the main
+
+88
+00:06:23,540 --> 00:06:26,660
+violence factor of this organism يبقى المتوقع أن
+
+89
+00:06:26,660 --> 00:06:30,960
+الـ vaccine هو هيستهدف أيضاً اللي هو الكابسول فأول
+
+90
+00:06:30,960 --> 00:06:35,520
+واحدة استبتت كوكاس ميمونيا vaccine وأول صحية ينتمي
+
+91
+00:06:35,520 --> 00:06:39,400
+إلى صنفين في السوق موجود منه vaccines واحد بحتوي
+
+92
+00:06:39,400 --> 00:06:43,880
+على 23 serotypes فاكريني الوقت قبل ما أشرفتش؟ تلاتة
+
+93
+00:06:43,880 --> 00:06:46,640
+وثمانين؟ أربعة وثمانين اتكلمت عنها على طول خلاص
+
+94
+00:06:46,640 --> 00:06:50,680
+أربعة وثمانين .. أربعة وثمانين stereotypes بس
+
+95
+00:06:50,680 --> 00:06:54,160
+احنا لازم نجد إيش منهم؟ تلاتة وعشرين هذا إيش
+
+96
+00:06:54,160 --> 00:06:57,740
+واصفه تلاتة وعشرين؟ the most common the most
+
+97
+00:06:57,740 --> 00:07:01,900
+prevalent الأكثر شيوعاً في المنطق ده مين اللي
+
+98
+00:07:01,900 --> 00:07:06,020
+بيعطوا هدول؟ بيعطوا لناس اللي فوق الستين سنة
+
+99
+00:07:06,020 --> 00:07:11,450
+تمام؟ اللي هم normal ما عندهمش أي مشكلة للي أعمارهم
+
+100
+00:07:11,450 --> 00:07:14,990
+أقل من ستين بس بيمتلكوا واحد أو أكثر من هذه الشروط
+
+101
+00:07:14,990 --> 00:07:20,730
+اللي احنا راح نتكلم عنها مثلاً عنده diabetes، عنده
+
+102
+00:07:20,730 --> 00:07:25,110
+serosis، تليف، عنده compromised spleen function،
+
+103
+00:07:25,110 --> 00:07:27,810
+يعني عنده مشكلة في وظيفة الـ spleen، أو عنده
+
+104
+00:07:27,810 --> 00:07:31,350
+sphenectomy، يعني الـ spleen تم استئصاله، تم
+
+105
+00:07:31,350 --> 00:07:35,390
+استئصاله وبطوحاه، تم استئصاله، هدول الناس لأ، إيه
+
+106
+00:07:35,390 --> 00:07:39,590
+ربما هم بياخدوا اللي هو الـ vaccine النوع الثاني من
+
+107
+00:07:39,590 --> 00:07:43,530
+الـ vaccines بيحتوي على عدد أقل بيحتوي على عدد أقل
+
+108
+00:07:43,530 --> 00:07:48,610
+وهم سبعة بس خدوا باركم it is coupled with a
+
+109
+00:07:48,610 --> 00:07:50,790
+carrier protein إيش يعني coupled with a carrier
+
+110
+00:07:50,790 --> 00:07:53,770
+protein؟ أنا بقول دام أنّ
+
+111
+00:07:56,090 --> 00:08:00,850
+لو هذا الـ antigen هو الـ protein بالمناسبة أو الـ
+
+112
+00:08:00,850 --> 00:08:02,770
+polysaccharide والـ polysaccharide أو الـ protein
+
+113
+00:08:02,770 --> 00:08:07,090
+they are antigenic لكن لأن الحجم matters يعني بيهم
+
+114
+00:08:07,090 --> 00:08:10,850
+الحجم في الـ antigenات زي ما تدرسوا في المناعة
+
+115
+00:08:10,850 --> 00:08:15,410
+ابتكروا أن لو ركّبت أنا هذا على carrier protein
+
+116
+00:08:15,410 --> 00:08:19,950
+بيصير حجمه أكبر صحيح هذا ما بيش أي قيمة من ناحية الـ
+
+117
+00:08:19,950 --> 00:08:24,210
+antigenات لكن القيمة لمين؟ بالانتيجين نفسه، لكن
+
+118
+00:08:24,210 --> 00:08:29,170
+كموليكول كله كبر، فصوت الـ immunogenicity بتاعته أعلى
+
+119
+00:08:29,170 --> 00:08:32,950
+وهذه عملية معروفة ومشهورة بنسمي المركب إما
+
+120
+00:08:32,950 --> 00:08:38,010
+conjugate أو adjuvant اللي هو مادة محفزة على الـ
+
+121
+00:08:38,010 --> 00:08:41,110
+antigenicity نفهم الحالة على فكرة نستخدم الـ
+
+122
+00:08:41,110 --> 00:08:44,730
+diphtheria toxoid كـ adjuvant أو كـ conjugate يعني
+
+123
+00:08:44,730 --> 00:08:49,130
+بنركب المركب اللي بنيها رام عالمين على diphtheria
+
+124
+00:08:49,130 --> 00:08:53,580
+toxoid يبقى الأولاني الـ conjugated والثاني
+
+125
+00:08:53,580 --> 00:08:58,320
+conjugated كم واحد الأولاني بيحتوي إيه؟ الثاني 
+
+126
+00:08:58,320 --> 00:09:05,860
+لمن يبقى في الثاني لأن أطفال اللي لم يستجيبوا بشكل 
+
+127
+00:09:05,860 --> 00:09:10,800
+جيد للـ vaccine الأولاني يبقى بتوقع أن الـ vaccine
+
+128
+00:09:10,800 --> 00:09:15,740
+الثاني الاستجابة اللي هو أفضل ليش أفضل؟ لأنه كما
+
+129
+00:09:15,740 --> 00:09:19,340
+حكينا conjugated with diphtheria
+
+130
+00:09:23,030 --> 00:09:27,230
+في مصطلح بنا نستخدمه الآن هنفهمه كويس قدر نركز مع
+
+131
+00:09:27,230 --> 00:09:33,110
+بعض و بدون كتاب المراجع أنتو أربعة و ثمانين أربعة
+
+132
+00:09:33,110 --> 00:09:37,170
+و ثمانين لما أنا ما أسألش أو ما أعطي محاضرة أو
+
+133
+00:09:37,170 --> 00:09:43,010
+ما أهتمش إلا بثلاثة و عشرين تمام؟ أو بلاش بثلاثة و
+
+134
+00:09:43,010 --> 00:09:47,830
+عشرين صار مضمونين مين اللي مش مضمونين؟ الباقين
+
+135
+00:09:48,430 --> 00:09:53,470
+بطلنا نهتم بالـ 23 فبقعدنا نهتمنا فيهم يردبوا و
+
+136
+00:09:53,470 --> 00:09:58,830
+يدوبوا و يخلصوا من المجتمع لكن الباقي موجودين
+
+137
+00:09:58,830 --> 00:10:03,290
+صحيح؟ يعني اللي لم نقتلهم بالـ vaccine وبالتالي
+
+138
+00:10:03,290 --> 00:10:07,470
+بنخلق من ما يسمى بـ Serotype Replacement أن اللي
+
+139
+00:10:07,470 --> 00:10:12,270
+ما اهتملاش فيهم بالـ vaccine يحلوا محل اللي اهتمنا
+
+140
+00:10:12,270 --> 00:10:16,330
+فيهم بالـ vaccine واضحة ممكن و مش واضحة أن أوضحها
+
+141
+00:10:16,330 --> 00:10:21,850
+كمان مرة عندي 23 نوع أو سلالة من البكتيريا كوضع لهم 
+
+142
+00:10:21,850 --> 00:10:24,850
+فاكسين هدولة هارفوا البلد كلهم مالوجلهم مش سوء أو
+
+143
+00:10:24,850 --> 00:10:29,410
+مكان يقعدوا فيه شهر perfection صح؟ إيش مصيرهم؟ إلى
+
+144
+00:10:29,410 --> 00:10:36,130
+زوالهم مين اللي بيضاروا؟ الباقين اللي مالهمش 
+
+145
+00:10:36,130 --> 00:10:40,970
+antivirus في أجسامنا ممكن أي من هؤلاء يحلوا محل
+
+146
+00:10:40,970 --> 00:10:45,430
+الواحد من الثلاثة و العشرين بظاهرة نسميها Serotype
+
+147
+00:10:45,430 --> 00:10:48,750
+Replacement نطمنكم في السطر الأخير أو الليلة
+
+148
+00:10:48,750 --> 00:10:53,870
+الأخيرة من اللوحة بتقول الثالث أن هذا التخوف اللي
+
+149
+00:10:53,870 --> 00:10:58,070
+هو Serotype Replacement هو تخوف نظري ولم نجد أي
+
+150
+00:10:58,070 --> 00:11:02,670
+بيانات لحد الآن تدعم هذا التخوف النظري لكن هي
+
+151
+00:11:02,670 --> 00:11:08,010
+ظاهرة منطقية .. منطقية متوقعة وما زالت to be
+
+152
+00:11:08,010 --> 00:11:11,870
+evaluated يعني باستمرار الأرواح دي بتطلعوا على هل
+
+153
+00:11:11,870 --> 00:11:16,050
+صار انتشار لسلالات جديدة غير الـ 23 أو غير السبعة
+
+154
+00:11:16,050 --> 00:11:19,040
+اللي احنا استخدمناهم أو لالكن لحد ما مافيش بيانات
+
+155
+00:11:19,040 --> 00:11:25,140
+تشير إلى أنّه ظاهرة مهمة و بتاعة نعترف من ال
+
+156
+00:11:25,140 --> 00:11:28,660
+capsular polysaccharide neisseria meningitidis
+
+157
+00:11:29,610 --> 00:11:35,310
+نييسيريا meningitis احنا اتكلمنا عنها و كنا بتعمل 
+
+158
+00:11:35,310 --> 00:11:38,570
+explosive .. explosive يعني مفجري .. explosive
+
+159
+00:11:38,570 --> 00:11:45,130
+epidemics و ذكرنا بعض النقاط الحساسة اللي وين ممكن
+
+160
+00:11:45,130 --> 00:11:49,250
+تنتشر فيها من ضمن أننا كنا college students طلاب
+
+161
+00:11:49,250 --> 00:11:53,830
+الجامعة اللي بيعيشوا في سكن داخلي و المجننين الجدر
+
+162
+00:11:53,830 --> 00:11:59,340
+و هتلاحظوا أن أكثر فئات لابنقصح إننا نعطيهم ال
+
+163
+00:11:59,340 --> 00:12:02,340
+vaccine، هم الفئتين اللي ذكرتهم الآن، احنا كمسلمين
+
+164
+00:12:02,340 --> 00:12:06,520
+لخاصية جمهنين .. مجتمع الو السودان .. مجتمع الو
+
+165
+00:12:06,520 --> 00:12:12,200
+السودان و احنا كمسلمين النيزة ليه الحج، إنه قبل
+
+166
+00:12:12,200 --> 00:12:16,750
+الحج بناخد هذا أيضا الـ vaccine اللي بيحتوي
+
+167
+00:12:16,750 --> 00:12:20,310
+الفاكسينة على أربع serotypes من الكبسول ولازم
+
+168
+00:12:20,310 --> 00:12:28,950
+نحفظهم الآن ACWY سهلين ACWY 135 والأخيرة Y ACWY 135
+
+169
+00:12:28,950 --> 00:12:32,330
+اللي هو Y هلأ الأربعة اللي موجودة برضه ليه منه
+
+170
+00:12:32,330 --> 00:12:38,310
+شكلين شكل الأول conjugated والتاني non conjugated
+
+171
+00:12:38,310 --> 00:12:39,570
+لإن اللي بيحتوي على ال
+
+172
+00:12:44,820 --> 00:12:47,460
+it is given when there is a high risk of
+
+173
+00:12:47,460 --> 00:12:51,280
+meningitis when
+
+174
+00:12:51,280 --> 00:12:56,900
+students enter college and are living in dormitory
+
+175
+00:12:56,900 --> 00:13:01,640
+when military recruits enter boot camp اللي هو
+
+176
+00:13:01,640 --> 00:13:05,000
+المعسكرات التدريب والتجنيد or for travelers
+
+177
+00:13:06,390 --> 00:13:08,590
+المسافرين اللي احنا من ضمنهم الحجاج اللي بيبقى
+
+178
+00:13:08,590 --> 00:13:12,310
+مسافرين to areas where meningitis الآن في مصطلح
+
+179
+00:13:12,310 --> 00:13:16,930
+أول مرضاه هنا المحاضر الماضي مر أو اللي جابنا كلمة
+
+180
+00:13:16,930 --> 00:13:21,750
+endemic endemic ميزنها عن epidemic المرض هو أنّه
+
+181
+00:13:21,750 --> 00:13:25,950
+hyper endemic wherein a disease incidence is
+
+182
+00:13:25,950 --> 00:13:30,790
+constantly high in one area يعني نسبة حدوث المرض
+
+183
+00:13:30,790 --> 00:13:32,210
+عالية في
+
+184
+00:13:35,630 --> 00:13:40,690
+نقول نسبة الإصابة سنوية مثلا 20 ألف إصابة لكل 100
+
+185
+00:13:40,690 --> 00:13:44,450
+ألف شخص هذه نسبة عالية جدا لكن سنويا بتحدث هذي
+
+186
+00:13:44,450 --> 00:13:49,150
+بيسموها hyper endemic hyper endemic يعني مستوطن
+
+187
+00:13:49,150 --> 00:13:53,490
+المرض و يحدث بنسبة عالية النوع الثالث بره من نجوم
+
+188
+00:13:53,490 --> 00:13:58,490
+الـ meningitis Hemophilus influenza و من ميزاتها أن
+
+189
+00:13:58,490 --> 00:14:02,890
+الكبسولة تبعتها is poorly immunogenic
+
+190
+00:14:05,990 --> 00:14:11,930
+لو حطيناها لحالها عند الإنسان للأسف بيكونش الإنسان
+
+191
+00:14:11,930 --> 00:14:15,970
+إلها أجسام مضادة بكميات كبيرة، لذا مافيش خيار
+
+192
+00:14:15,970 --> 00:14:20,010
+قدامها لأنه يكون conjugated و unconjugated، لازم
+
+193
+00:14:20,010 --> 00:14:25,310
+يكون مفلوس influenza vaccine contains the type B
+
+194
+00:14:25,310 --> 00:14:29,790
+polysaccharideالـ type B polysaccharide يبقى فيه
+
+195
+00:14:29,790 --> 00:14:34,210
+لنا strand واحد conjugated to diphtheria toxoid or
+
+196
+00:14:34,210 --> 00:14:39,130
+other carrier proteins يبقى فيه لنا conjugation
+
+197
+00:14:39,130 --> 00:14:42,510
+لإما diphtheria اللي هي اللي conjugated أو other
+
+198
+00:14:42,510 --> 00:14:46,130
+proteins it is given to children between the ages
+
+199
+00:14:46,130 --> 00:14:47,950
+of two to fifteen months
+
+200
+00:14:51,880 --> 00:14:56,700
+و قلتلكوا إن العمر حتى جدول الـ vaccines في العالم
+
+201
+00:14:56,700 --> 00:15:02,180
+بتغير كل ما بتتراكم لنا بيانات جديدة بتحسن من
+
+202
+00:15:02,180 --> 00:15:05,820
+فهمنا لبعض الأمور ممكن نغيّر في جناح ال
+
+203
+00:15:05,820 --> 00:15:12,480
+vaccination فلما أنا أقول البداية من شهرين لحد 15
+
+204
+00:15:12,480 --> 00:15:15,380
+شهر يمكن هذه الفترة اللي بتسبق
+
+205
+00:15:17,970 --> 00:15:21,990
+الزمن الملائم للبكتيريا أن تحدث فيه أمراض يعني
+
+206
+00:15:21,990 --> 00:15:25,870
+أكثر ناس بيصاروا بالأمراض هم الأطفال اللي فيها
+
+207
+00:15:25,870 --> 00:15:30,170
+السن و بعدها بشوية أنّه تابتنها قبل ما تبدأ هذه
+
+208
+00:15:30,170 --> 00:15:36,870
+المرحلة الخطرة بنعطي الـ vaccine زي ما حكينا قبل
+
+209
+00:15:36,870 --> 00:15:39,590
+لحظات أنّه poorly immunogenic و it should be
+
+210
+00:15:39,590 --> 00:15:42,810
+coupled to بقولنا كده protein to enhance
+
+211
+00:15:42,810 --> 00:15:44,150
+immunogenicity
+
+212
+00:15:46,100 --> 00:15:51,120
+الآن احنا بنعرف فاكسين اسمه DPT صح؟ DPT ثلاثي،
+
+213
+00:15:51,120 --> 00:15:54,680
+Diphtheria Pertussis و Tetanus، فهي فاكسين الآن
+
+214
+00:15:54,680 --> 00:16:01,240
+رباعي في الأسواق، بيشمل الثلاثة بالإضافة إلى ال
+
+215
+00:16:01,240 --> 00:16:06,040
+Hemophilus influenza type B فصار رباعي، صار عشانها
+
+216
+00:16:06,040 --> 00:16:11,450
+فاكسين رباعي خلصنا .. خدوا بالكوا دقا .. خلصنا
+
+217
+00:16:11,450 --> 00:16:16,250
+المعلومات المتعلقة بثلاث ميكروبيات Spetacoccus
+
+218
+00:16:16,250 --> 00:16:20,410
+pneumonia, Muflis influenza و Nacinamide bites
+
+219
+00:16:20,410 --> 00:16:27,020
+اللي بتكلم فيه مقصود عن البكتيريا الرقم ثلاثة بتعرفوا
+
+220
+00:16:27,020 --> 00:16:30,140
+السالمونيلا التيفية؟ السالمونيلا التيفية إلها
+
+221
+00:16:30,140 --> 00:16:33,520
+vaccine و لو رجعتوا للمحاضرة تبعتنا هنقولوا ال
+
+222
+00:16:33,520 --> 00:16:37,260
+prevention by vaccine طبعا الآن الـ vaccine طبعا
+
+223
+00:16:37,260 --> 00:16:41,420
+السالمونيلا التيفية في منه نوعين واحد بيستهدف
+
+224
+00:16:41,420 --> 00:16:46,480
+الكبسول فنحطه مع مين؟ مع الكبسول، مع دولة واحد
+
+225
+00:16:46,480 --> 00:16:50,300
+لكل البكتيريا كل البكتيريا اللي هي killed bacteria
+
+226
+00:16:50,300 --> 00:16:55,620
+killed bacteria يعني السالمونيلا ممكن نكون واحد أو
+
+227
+00:16:55,620 --> 00:16:56,180
+ندمجها 
+
+228
+00:17:02,020 --> 00:17:07,740
+طبعا تسمونه طيفي vaccine is indicated يوصف ليه أو
+
+229
+00:17:07,740 --> 00:17:12,820
+يوصى بيه لمين to people who are traveling to areas
+
+230
+00:17:12,820 --> 00:17:17,620
+where typhoid fever is common يعني الحمى التيفوئيد
+
+231
+00:17:17,620 --> 00:17:24,240
+كان موجود أو منتشر بشكل كبير أو لأشخاص who are in
+
+232
+00:17:24,240 --> 00:17:28,420
+contact with typhoid fever patients or carriers
+
+233
+00:17:29,860 --> 00:17:36,620
+عشان احنا عارفين أن الانتقال في كالقول و الاحتكاك
+
+234
+00:17:36,620 --> 00:17:41,080
+المباشر مع المرضى قد يعني أنت عارفين given aseptic
+
+235
+00:17:41,080 --> 00:17:47,520
+technique لو راح تخيلها عملية معقدة جدا و مهما ببنى
+
+236
+00:17:47,520 --> 00:17:50,540
+من جهد .. مهما ببنى من جهد في الـ aseptic technique
+
+237
+00:17:50,540 --> 00:17:55,300
+إلا يصير فيه breach خلل معين وبالتالي قد ينتقل
+
+238
+00:17:55,300 --> 00:17:59,960
+المرض فلما أنا أكون بتعامل مع مرضى و أنا at high
+
+239
+00:17:59,960 --> 00:18:05,590
+risk لما ممكن أاخد إيه؟ vaccine في هذه الحلقة النوع
+
+240
+00:18:05,590 --> 00:18:07,710
+الثالث من الـ vaccine دو اللي عنه هو الـ toxoid
+
+241
+00:18:07,710 --> 00:18:11,090
+vaccine و الـ toxoid زي ما حكينا هي عبارة عن toxin
+
+242
+00:18:11,090 --> 00:18:15,010
+that has been modified كلمة modified قد تكون ال
+
+243
+00:18:15,010 --> 00:18:19,170
+modification chemical قد تكون physical قد تكون
+
+244
+00:18:19,170 --> 00:18:22,050
+genetic engineering يعني ممكن نعمل modification
+
+245
+00:18:22,050 --> 00:18:27,750
+بالهندسة الوراثية ناخد أول مثال Cornebacterium
+
+246
+00:18:27,750 --> 00:18:32,870
+diphtheria vaccine حد سمع منكوا أن فلان أصيب بال
+
+247
+00:18:32,870 --> 00:18:37,400
+diphtheria مافيش، ليش مافيش؟ لأن الطلاب العربية
+
+248
+00:18:37,400 --> 00:18:41,320
+indicated يعني immunization against diphtheria is
+
+249
+00:18:41,320 --> 00:18:44,940
+indicated for every child، يعني حضراتكم لما كنتوا
+
+250
+00:18:44,940 --> 00:18:49,520
+child، كنتوا children، أخدتها اللقاح كل العالم 
+
+251
+00:18:49,520 --> 00:18:54,860
+اللي بيصلّوا .. اللي بيفطّوا الحضارة بياخدوا الـDPT
+
+252
+00:18:54,860 --> 00:18:59,100
+كنزها من الفكس، لذلك نسبة الدفتيريا تقريبا .. مش 
+
+253
+00:18:59,100 --> 00:19:03,540
+تقريبا في غزة، لها من سنوات صفر، لها من سنوات
+
+254
+00:19:03,540 --> 00:19:10,560
+صفر، يعني الـToxoid لحاله كان كفيل بإنه يحمينا من 
+
+255
+00:19:10,560 --> 00:19:13,240
+الـ .. الـ .. الـ .. الـ .. هذا .. هذا نقطة من فكرة
+
+256
+00:19:24,760 --> 00:19:28,480
+أنا بحكي كـToxoid مش كـfragment من البكتيريا أخرى،
+
+257
+00:19:28,480 --> 00:19:30,840
+بس لأه .. لأن هو أصلا راح نكون نستخدمها كـكاريّا،
+
+258
+00:19:30,840 --> 00:19:34,840
+فهي بكتيريا كبيرة، هو كبير، فبتاعه immunogenic جيد،
+
+259
+00:19:34,840 --> 00:19:39,700
+immunogenic جيد، أنا فهمت سؤالك لأه متأخرة، إنه ..
+
+260
+00:19:39,700 --> 00:19:43,680
+هو أنا بـ .. بـ .. بتكلم على الـfragment، إنه أنا
+
+261
+00:19:43,680 --> 00:19:48,020
+خدت Toxoid لحاله، ما أخدتش معاه مركبات أخرى كبسول
+
+262
+00:19:48,020 --> 00:19:50,340
+ولا خدت معاه أنتجلات ثانية
+
+263
+00:19:54,090 --> 00:19:57,070
+كده المرة عرفنا إنه بيعطل منين؟ for every child
+
+264
+00:19:57,070 --> 00:20:02,010
+على تلت جرعات، اتنين، أربع أشهر من العمر، وبعد سنة
+
+265
+00:20:02,010 --> 00:20:05,570
+من آخر جرعة، يعني بيسمّوها booster dose اللي هي
+
+266
+00:20:05,570 --> 00:20:09,250
+الجرعة الداعمة أو الجرعة التنشيطية اللي هي اسمها
+
+267
+00:20:09,250 --> 00:20:13,710
+باللغة العربية أما داعمة وهي الصحيحة الترجمة
+
+268
+00:20:13,710 --> 00:20:17,030
+الحرفية هي الـboost معناها دعم، فـbooster dose لكن
+
+269
+00:20:17,030 --> 00:20:21,190
+احنا برضه طبيًا بيطلق عليها الجرعة التنشيطية اللي
+
+270
+00:20:21,190 --> 00:20:27,490
+بتذكّر جهاز المناعة، رقم اتنين طبعًا، لحظة، الأولاني اللي
+
+271
+00:20:27,490 --> 00:20:31,810
+هو في الجوجل الأولى، it is formaldehyde treated، إيش
+
+272
+00:20:31,810 --> 00:20:35,730
+يعني formaldehyde treated يعني معالج بمادة اللي هو
+
+273
+00:20:35,730 --> 00:20:39,990
+الـformaldehyde، رقم اتنين Clostridium Tetani أو 
+
+274
+00:20:39,990 --> 00:20:43,150
+Clostridium Tetani vaccine contains tetanus toxoid
+
+275
+00:20:43,150 --> 00:20:46,970
+and is given to everyone زي نفسي ما هو الدول
+
+276
+00:20:46,970 --> 00:20:52,160
+الفلّافية early in life، وممكن بعد فترة، وكل فترة يعني
+
+277
+00:20:52,160 --> 00:20:54,820
+هو غالبًا بيعطي protection لخمس سنوات لو أعطينا
+
+278
+00:20:54,820 --> 00:21:00,540
+كمان جرعة على خمس سنوات، فبينشّط اللي لازم الآن
+
+279
+00:21:00,540 --> 00:21:04,340
+لاحظوا الأول والثاني Toxoid، Toxoid، وكانوا بالفعل
+
+280
+00:21:04,340 --> 00:21:08,100
+تتنسّع إننا تقريبًا شبه zero بياخدوا شبه zero، تيجي
+
+281
+00:21:08,100 --> 00:21:10,560
+للثالثة اللي هي الـborderline laparotosis
+
+282
+00:21:10,560 --> 00:21:10,940
+vaccine
+
+283
+00:21:13,620 --> 00:21:17,240
+but includes other proteins as well، خدوا بالكم،
+
+284
+00:21:17,240 --> 00:21:23,700
+اللي في غزة DBT، والـDBT هي Deuterium Pertosis
+
+285
+00:21:23,700 --> 00:21:27,980
+Tetanus، والثلاثة دوكسول فقط، لالي، we are protected
+
+286
+00:21:27,980 --> 00:21:32,040
+من الـvaccine هذا، يعني حمينا من الـDeuterium و
+
+287
+00:21:32,040 --> 00:21:35,260
+حمينا من الـTetanus، لكن we are not totally
+
+288
+00:21:35,260 --> 00:21:41,020
+protected من اللي هو الـ .. الـ .. الـ .. الـ Pertosis، وأكبر دي
+
+289
+00:21:41,020 --> 00:21:43,920
+عيبالك إن كل اللي عالم اللي بيستخدم هذا الـvaccine
+
+290
+00:21:43,920 --> 00:21:47,380
+برضه مش protected against this disease، وكان في
+
+291
+00:21:47,380 --> 00:21:51,620
+الكيان المحتلّ للغاصب كان قبل عدة سنوات ممكن
+
+292
+00:21:51,620 --> 00:21:55,840
+خمسمائة حالة سجّلوه من الـpertosis، وإن في غزة في
+
+293
+00:21:55,840 --> 00:22:00,140
+symptoms typical pertosis لكن الأطباء يبدو إنهم مش
+
+294
+00:22:00,140 --> 00:22:03,920
+مقتنعين إن في عندنا ذات بقى الـpertosis، فطبعًا هو
+
+295
+00:22:03,920 --> 00:22:08,260
+الـpertosis self-limiting على الرغم من خطورته
+
+296
+00:22:08,260 --> 00:22:12,780
+وصعوبته إلا إنه حتى لو ما أعطينا علاج غالبًا
+
+297
+00:22:12,780 --> 00:22:16,120
+بالنسبة لي لو كنت فيه ضعيفة جدًا، قادرة جدًا أستاذ
+
+298
+00:22:16,120 --> 00:22:19,900
+باللي متعرف بينا، شو هو الـpertosis؟ هو السعال
+
+299
+00:22:19,900 --> 00:22:22,840
+الديكي، السعال الديكي، بعمل كل شيء أنا بسمّيه السعال
+
+300
+00:22:22,840 --> 00:22:28,790
+الديكي، ما نتّش كذا؟ الـ .. الـdisease بيتكون .. عشان
+
+301
+00:22:28,790 --> 00:22:32,310
+ذلك لازم نشرح لأولادنا مولودة من فهم أعمق يا بنات، فهم
+
+302
+00:22:32,310 --> 00:22:36,590
+أعمق للمتباينة الـgenesis أو لأي مسألة في الدنيا
+
+303
+00:22:36,590 --> 00:22:41,330
+بيقدر يتّخذ قرار أفضل، وأنا دايمًا بقول مثال، بقول
+
+304
+00:22:41,330 --> 00:22:48,210
+إن الشخص في المنزل قد يعني يتعرّض لقرار يعتبره
+
+305
+00:22:48,210 --> 00:22:54,870
+ظالم من ربّ الأسرة من الوالد، يعتبره ظالم وغير راضي
+
+306
+00:22:54,870 --> 00:22:59,160
+عن هذا القرار، وأنا بقول إنّه لأ، يعني لو أنت عرفت
+
+307
+00:22:59,160 --> 00:23:03,560
+المعلومات اللي عند ربّ الأسرة ربما اتّخذت نفس القرار
+
+308
+00:23:03,560 --> 00:23:07,480
+وغالبًا .. ده بتـ .. ممكن بتلاحظوا الأمهات
+
+309
+00:23:07,480 --> 00:23:13,410
+بتعلّم البنات وبيقولوا لهم غلط، وبيقولوا لهم صح، تمام؟ طبعًا
+
+310
+00:23:13,410 --> 00:23:17,230
+لو رجعنا بالتاريخ ما نلقى كافية من أية بنت كانت
+
+311
+00:23:17,230 --> 00:23:20,170
+أما تقولها نفس الكلام اللي هي بتقولكوا إياه، وهي ما
+
+312
+00:23:20,170 --> 00:23:22,970
+تسمعش الكلام، وهي ما كانتش تسمع الكلام، يعني سلسلة
+
+313
+00:23:22,970 --> 00:23:26,730
+من إيه، لكن اللي أنا بوصله إياه إنّه من يمتلك
+
+314
+00:23:26,730 --> 00:23:30,850
+المعلومات يمتلك المعلومات في شكل عام، مش ضروري فيه
+
+315
+00:23:30,850 --> 00:23:32,970
+دراسة يعني، وعندها كل المعلومات موجودة، لكن بتاخد
+
+316
+00:23:32,970 --> 00:23:37,320
+قرار خاطئ، لكن هم اللي بيمتلكوا معلومات كثيرة غالبًا
+
+317
+00:23:37,320 --> 00:23:42,880
+بياخد قراره مبني على قاعدة أوسع من المعرفة، فبالتالي
+
+318
+00:23:42,880 --> 00:23:48,680
+بيكون أفضل، ليش بنحكي الكلام هذا؟ بنحكي الكلام هذا
+
+319
+00:23:48,680 --> 00:23:52,880
+جاب الـ .. طلبات، اتكلمت عن الـbacteroidis
+
+320
+00:24:02,710 --> 00:24:07,310
+أنا عندي نية إن أنا أقوم بمحاضرة عامة عن الـnormal
+
+321
+00:24:07,310 --> 00:24:11,030
+flora وعن الخير اللي بيحصل منها، لحظة تخيلوا
+
+322
+00:24:11,030 --> 00:24:15,830
+فصائل الدم is modified by normal flora، diabetes،
+
+323
+00:24:15,830 --> 00:24:19,630
+مرض السكر قد ينطوي عليه، فيه دراسات ومؤشرات تقول إن
+
+324
+00:24:19,630 --> 00:24:23,650
+normal flora لها علاقة بهلوتة، ونسبة انتشاره
+
+325
+00:24:24,440 --> 00:24:28,300
+المزاج تبعنا، إذا كان كويس أو مش كويس، إليه علاقة
+
+326
+00:24:28,300 --> 00:24:32,240
+بالنظر في الورق، نعم، فيعني إذا كانت تلعب بقى
+
+327
+00:24:32,240 --> 00:24:36,700
+بميرتنا وبتغيّر من الـmood تبعتنا، فبتجرب اللي ..
+
+328
+00:24:36,700 --> 00:24:40,340
+اللي .. اللي .. اللي بيخليك كده من الكلام إن
+
+329
+00:24:40,340 --> 00:24:46,650
+الإنسان غالبًا بيـ .. بيحاول يقطف ثمار المعرفة مبكّرًا،
+
+330
+00:24:46,650 --> 00:24:50,550
+بمعنى إنه بس يعرف معرفة جديدة، بيحاول يستغلّها،
+
+331
+00:24:50,550 --> 00:24:54,470
+فمثلاً، عرفنا إن البكتيريا هي اللي بتسيب الأمراض،
+
+332
+00:24:54,470 --> 00:25:01,020
+kill them all، اقتلوها، 99% بتستخدمها في 99.9% اللي
+
+333
+00:25:01,020 --> 00:25:05,580
+بتقول تمام؟ طهّر يدك، طهّر رجليك، أهمال أكثر، خلاء
+
+334
+00:25:05,580 --> 00:25:09,020
+انتباطيك، وفي الآخر نكتشف إنه هذه الإجراءات
+
+335
+00:25:09,020 --> 00:25:13,260
+بتسبب لنا أضرار، مصبوط أو مش مصبوط؟ طبعًا الخير ..
+
+336
+00:25:13,260 --> 00:25:17,500
+الخير في Noble Flora ناتج عن التغيّرات اللي .. اللي
+
+337
+00:25:17,500 --> 00:25:19,940
+.. اللي الإنسان في الغذاء، والتغيّرات في الأدوية، و
+
+338
+00:25:19,940 --> 00:25:22,540
+في العلاجات، وفي .. في .. في .. وفي طريق الـlife
+
+339
+00:25:22,540 --> 00:25:25,140
+style تبعه، وأنا بتقولكوا مرة مهمة، أنا بتقولكوا
+
+340
+00:25:25,140 --> 00:25:29,940
+إنّه تغيير النظام اللي بيده إلى الأبقار أدّى .. ده
+
+341
+00:25:29,940 --> 00:25:33,060
+الأبقار .. أدّى كده إنه يصير جزء من النظام اللي هو
+
+342
+00:25:33,060 --> 00:25:39,380
+تلاتة equalized أو 15787، يبقى فعلًا من العميق
+
+343
+00:25:39,380 --> 00:25:47,220
+بيعطينا إمكانية التدخل بدون ما نقضي الـ .. الـ .. الـ
+
+344
+00:25:47,220 --> 00:25:49,860
+.. الـ .. الـ .. الـ البيئة، ونقضي الـ .. الـ .. الـ .. الـ
+
+345
+00:25:49,860 --> 00:25:53,220
+.. الـbalance اللي على مدار القرون موجود
+
+346
+00:25:53,220 --> 00:25:58,120
+البورميلابيكوس، كنا فاكرين إن الـtoxoid أو الـtoxin
+
+347
+00:25:58,120 --> 00:26:02,220
+هو الـmajor phytonic component of the bacteria
+
+348
+00:26:05,100 --> 00:26:08,800
+في الدفتيريا كرابنا صحيح، في التتروس كرابنا صحيح، لكن
+
+349
+00:26:08,800 --> 00:26:13,020
+في كرابنا مش صحيح، لإنه لجهر البكتيريا اللي ها هيم
+
+350
+00:26:13,020 --> 00:26:17,920
+أجلوتينين بيتّصق في السيليا اللي موجودة في التراكيا
+
+351
+00:26:17,920 --> 00:26:21,100
+وبتنقل خلال ما زمانا قلنا في حركة السيليا
+
+352
+00:26:21,100 --> 00:26:25,920
+reaction اللي هو أساس طرد السبيوتم اللي بيخرج من
+
+353
+00:26:25,920 --> 00:26:30,520
+رئة الإنسان، وبالتالي اللقاح الجديد بيحتوي على
+
+354
+00:26:31,260 --> 00:26:35,820
+بيحتوي على فلامنتاس هم أجلوينين والبرتكتين، و
+
+355
+00:26:35,820 --> 00:26:39,460
+دلوقتي المركّبين اللي هم علاقة بالبعض في نفس
+
+356
+00:26:39,460 --> 00:26:43,340
+الجدران البكتيرية، فالآن الـvaccine اللي موجود في
+
+357
+00:26:43,340 --> 00:26:50,960
+أمريكا بيحتوي على الـToxoid بقى كما هو زائد اللي هو
+
+358
+00:26:50,960 --> 00:26:54,660
+الـفلامنتاس هم أجلوينين زائد اللي هو البرتكتين
+
+359
+00:26:54,660 --> 00:26:56,840
+اللي هم الـcomponents of the cell wall
+
+360
+00:27:00,320 --> 00:27:04,300
+هنبدأ إن شاء الله مشوار السيمينارات للطالبات، كلّ
+
+361
+00:27:04,300 --> 00:27:08,600
+طالبة هتقدّم خمس دقائق موضوع إن شاء الله يعني جديد
+
+362
+00:27:08,600 --> 00:27:12,120
+في الـmicrobiology، ليس بضرورة يكونوا علاقة في الـ
+
+363
+00:27:12,120 --> 00:27:15,260
+medical microbiology، لكن على الأقل بوسع مداركنا
+
+364
+00:27:15,260 --> 00:27:19,080
+ومفاهيمنا، وهنبدأ إن شاء الله اليوم مع نور الصفة
+
+365
+00:27:19,080 --> 00:27:24,320
+دي، أول presentation اللي هتحاول تقدّمها خلال خمس
+
+366
+00:27:24,320 --> 00:27:24,380
+دقائق
+
+367
+00:27:29,230 --> 00:27:32,850
+أول اسم البكتيريا موجودة في كل مكان، موجودة في
+
+368
+00:27:32,850 --> 00:27:36,150
+أجسامنا، في النبات، في الحيوانة، في كل مكان، منها
+
+369
+00:27:36,150 --> 00:27:39,450
+ما هو مفيد، منها ما هو ضارّ، اليوم راح أحكيلكم عن
+
+370
+00:27:39,450 --> 00:27:42,610
+إحدى فوائد البكتيريا، إن البكتيريا بتنتج لنا
+
+371
+00:27:42,610 --> 00:27:46,150
+إلكترونات، عن طريق هذه الإلكترونات بتستطيع إنولد
+
+372
+00:27:46,150 --> 00:27:50,050
+كهربا، فسمّوها هذه البكتيريا fuel microorganism
+
+373
+00:27:50,050 --> 00:27:56,470
+buttery bacteria، هذه البكتيريا من .. من أنواع هذه
+
+374
+00:27:56,470 --> 00:27:59,190
+البكتيريا، نسأل على هذه البكتيريا اللي هي بكتيريا
+
+375
+00:27:59,190 --> 00:28:02,830
+الـRodiferrix ferradinaceae، هذه البكتيريا هي من 
+
+376
+00:28:02,830 --> 00:28:06,030
+عائلة الـ common denisius هذه من الـ genius
+
+377
+00:28:06,030 --> 00:28:10,590
+Rodiferrix species ferradinaceae هذه البكتيريا أول
+
+378
+00:28:10,590 --> 00:28:13,290
+مرة عزلوها من رواسف Vanguela
+
+379
+00:28:17,760 --> 00:28:21,760
+هذه البكتيريا لها خصائص تجعلها مختلفة عن باقي
+
+380
+00:28:21,760 --> 00:28:25,660
+البكتيريات، هذه أن أثناء عملية تنفسها تستخدم
+
+381
+00:28:25,660 --> 00:28:29,280
+الحديد في التنفس تبعها، و تتغذى زي أي نوع من
+
+382
+00:28:29,280 --> 00:28:35,500
+أنواع البكتيريا على الجلوكوز، السليلوز، السكروز هذه
+
+383
+00:28:35,500 --> 00:28:39,520
+البكتيريا أنه زي ما قلت قبلها أنه بتنتج إلكترونات
+
+384
+00:28:39,520 --> 00:28:43,740
+عن طريق تستطيع أن تولد كهرباء من خلالها هلقيت
+
+385
+00:28:43,740 --> 00:28:48,520
+كيف هي راح تولد الكهرباء؟ قلت أنه هي بتستخدم أثناء
+
+386
+00:28:48,520 --> 00:28:52,880
+عملية التنفس الحالية و بتستخدم كمان الجلوكوز كمصدر
+
+387
+00:28:52,880 --> 00:28:56,180
+للغذاء لها، بتروح عن طريقهم، بتكسّرها فبتنتج
+
+388
+00:28:56,180 --> 00:29:00,310
+إلكترونات، هذه الإلكترونات بتعطينا كهرباء وبتفضل أن
+
+389
+00:29:00,310 --> 00:29:03,930
+تعمل هذه العملية عن درجة حرارة من 4 درجة سيليزية ل
+
+390
+00:29:03,930 --> 00:29:07,830
+30 درجة سيليزية ولكن الـ optimum لها 25 درجة
+
+391
+00:29:07,830 --> 00:29:12,890
+سيليزية كيف أن نكشف عنها هي مبدأ العمل سكر جلوكوز
+
+392
+00:29:12,890 --> 00:29:17,410
+مع ماء بوجود الحديد رح يعطينا CO2 مع الحديد نفس
+
+393
+00:29:17,410 --> 00:29:20,910
+الشغل مع أيونات الهيدروجين وحيكون الإلكترونات هي
+
+394
+00:29:20,910 --> 00:29:25,390
+عبارة عن أولى عشان نفهم مبدأ العمل رح أورّجيكم 
+
+395
+00:29:25,390 --> 00:29:25,790
+فيديو
+
+396
+00:29:29,330 --> 00:29:32,610
+أول شغلة أن النبات راح يعمل عمليات بناء الضوء
+
+397
+00:29:32,610 --> 00:29:49,490
+راح ينتج من خلاله سكر الجلوكوز الـ 
+
+398
+00:29:49,490 --> 00:29:52,030
+macro organism نعتبرها اللي هي الـ Rodoflax في الـ
+
+399
+00:29:52,030 --> 00:29:56,030
+ردينيز راح تتغذى عليها فرح تنتج لنا الـ CO2 و رح
+
+400
+00:29:56,030 --> 00:29:57,530
+تنتج لنا إلكترونات
+
+401
+00:30:08,420 --> 00:30:11,520
+هلقيت رح نحطها نعتبر أن احنا حطيناها في جهاز
+
+402
+00:30:11,520 --> 00:30:14,120
+بيحتوي على أنود و على كاثود و على الـ membrane
+
+403
+00:30:14,120 --> 00:30:17,040
+المادة اللي جرافيك الـ membrane المادة اللي جرافيك
+
+404
+00:30:17,040 --> 00:30:20,340
+وظيفته أن يسهل عملية نقل الأيونات الهيدروجين من
+
+405
+00:30:20,340 --> 00:30:24,060
+ناحية الأنود لناحية الكاثود هلقيت الإلكترونات بما
+
+406
+00:30:24,060 --> 00:30:28,240
+أنها positive charge رح .. negative charge رح
+
+407
+00:30:28,240 --> 00:30:32,700
+تنتقل عن الأنود لمصباح الكهربائي عشان نشوف إذا هي
+
+408
+00:30:32,700 --> 00:30:34,600
+هتولد من الكهرباء ولا مش هتولد
+
+409
+00:30:54,390 --> 00:30:57,810
+بما أن الضوء يعني أش تحتوى على إلكترونات و أنها
+
+410
+00:30:57,810 --> 00:31:06,110
+فعلاً بتولد كهرباء راح ترجع طبعاً على الـ cathode و
+
+411
+00:31:06,110 --> 00:31:09,930
+راح تربط ما بين الـ oxygen و الـ hydrogen اللي انتقل
+
+412
+00:31:09,930 --> 00:31:13,910
+من الأنود للـ cathode و بتنتج لنا من خلاله ماء هذه
+
+413
+00:31:13,910 --> 00:31:15,770
+المبدأ العملية بشكل مختصر
+
+414
+00:31:23,740 --> 00:31:26,720
+وهي شكل العملية مرة ثانية أن قانون .. إجت
+
+415
+00:31:26,720 --> 00:31:29,860
+البكتيريا أنتجت لنا إلكترونات من القانون لنصبح
+
+416
+00:31:29,860 --> 00:31:34,380
+الكهربائي للكاثود فنتجت لنا بعدها ماء شو المستقبل
+
+417
+00:31:34,380 --> 00:31:37,300
+هذه البكتيريا؟ هذه البكتيريا لو أجلنا بدنا نقارنها
+
+418
+00:31:37,300 --> 00:31:40,920
+بينها و بين كمية الكهرباء اللي بتنتج في العالم طبعاً
+
+419
+00:31:40,920 --> 00:31:44,140
+و بين الشركات طبعاً الكمية بالنسبة لها كمية قليلة
+
+420
+00:31:44,140 --> 00:31:48,600
+بس الـمهم أن هي البكتيريا هاي بتساعد على
+
+421
+00:31:48,600 --> 00:31:54,040
+إنتاج الكهرباء نتخيل شوية أن هذه الكهرباء وصلت عندنا
+
+422
+00:31:54,040 --> 00:31:57,300
+لغزة .. اه هذه البكتيريا وصلت عندنا لغزة و احنا
+
+423
+00:31:57,300 --> 00:32:00,420
+أملنا أن الكهرباء بدل ما تشير تجينا 8 ساعات تشير
+
+424
+00:32:00,420 --> 00:32:04,300
+على الأقل تجينا 10 ساعات، 12 ساعة فهذه البكتيريا
+
+425
+00:32:04,300 --> 00:32:09,040
+وصلت عندنا وقدرنا نستغلها بشكل صحيح ووفرنا الأجهزة و
+
+426
+00:32:09,040 --> 00:32:12,360
+الإمكانيات من خلالها فنتجت لنا الكهرباء و في نفس
+
+427
+00:32:12,360 --> 00:32:17,580
+الوقت في بكتيريا ثانية اسمها Chuanella Undecinens
+
+428
+00:32:17,580 --> 00:32:21,360
+هذه البكتيريا برضه موجودة في مياه المجاري هذه
+
+429
+00:32:21,360 --> 00:32:25,300
+البكتيريا لو أيضاً قدرنا نعزلها بتشتغل بنفس مبدأ
+
+430
+00:32:25,300 --> 00:32:30,560
+العمل و بتستطيع أن تنتج لنا كهرباء فلو وصلت لغزة مرة
+
+431
+00:32:30,560 --> 00:32:34,700
+ثانية و استغلناها بشكل صحيح هترجع مرة ثانية لغزة
+
+432
+00:32:34,700 --> 00:32:40,470
+تكون موضوع شكر بسم الله الرحمن الرحيم اليوم
+
+433
+00:32:40,470 --> 00:32:43,930
+موضوعنا هيتحدث عن نوع من أنواع البكتيريا بعمل على
+
+434
+00:32:43,930 --> 00:32:50,550
+تحويل فصائل الدم المختلفة من A أو B أو AB إلى
+
+435
+00:32:50,550 --> 00:32:53,890
+الفصيلة O باستعمال نوعين من أنواع البكتيريا الـ
+
+436
+00:32:53,890 --> 00:32:57,990
+bacterium fragilis and النوع الثاني هو عبارة عن
+
+437
+00:32:57,990 --> 00:33:01,210
+الـ flavobacterium meningospectum وله اسم آخر وهي
+
+438
+00:33:01,210 --> 00:33:03,730
+عبارة أو الاسم بتاعها مشهورة فيه هي
+
+439
+00:33:03,730 --> 00:33:06,710
+elizabethkingia meningospectum هذا هو النوع الأول
+
+440
+00:33:06,710 --> 00:33:12,330
+من البكتيريا bacteria it's pressure loss هي الأنواع
+
+441
+00:33:12,330 --> 00:33:15,810
+أو الخصائص اللي تتمتع بها هذا النوع من البكتيريا
+
+442
+00:33:15,810 --> 00:33:19,810
+طبعاً obligate aerobic واخذنا في مصطلح عن معنى
+
+443
+00:33:19,810 --> 00:33:22,470
+obligate aerobic و قعدين الـ gram negative بصبغة
+
+444
+00:33:22,470 --> 00:33:25,610
+وهي الصورة موضحة لنا تحت الـ microscope بعد ما عملنا
+
+445
+00:33:25,610 --> 00:33:28,990
+لها stain و بتأكد لنا أنها negative بصبغة و هي عبارة
+
+446
+00:33:28,990 --> 00:33:31,810
+عن normal flora موجودة in intestinal tract in
+
+447
+00:33:31,810 --> 00:33:36,270
+human كولونز بتكون في حالتها الطبيعية بتبقى flora
+
+448
+00:33:36,270 --> 00:33:39,410
+microflora أو normal flora لكن في حالة الـ abnormal
+
+449
+00:33:39,410 --> 00:33:42,570
+conditions فوراً بتتحول إلى infections بتسبب الـ
+
+450
+00:33:42,570 --> 00:33:46,130
+endogenous infections طبعاً هي غير متحركة و ليست من
+
+451
+00:33:46,130 --> 00:33:48,230
+أنواع الـ endosporous لأن الـ endosporous هي كانت
+
+452
+00:33:48,230 --> 00:33:51,210
+لها نوعين مشهورين، فيهم هي عبارة عن الفاسيغاس
+
+453
+00:33:51,210 --> 00:33:54,990
+و الباستريديا طبعاً هي أيضاً من أنواع البكتيريا
+
+454
+00:33:54,990 --> 00:33:58,990
+المقاومة للبنسلين حيث تمت على إنتاج لـ beta
+
+455
+00:33:58,990 --> 00:34:04,220
+-lactamase أيضاً نستطيع عزلها عن طريق نوع من أنواع
+
+456
+00:34:04,220 --> 00:34:07,140
+الـ agar هو عبارة عن الـ bacterium spill esculin
+
+457
+00:34:07,140 --> 00:34:11,020
+agar يرمز بالرمز BBA هذا النوع من البكتيريا بتروح
+
+458
+00:34:11,020 --> 00:34:16,600
+بتحوّلها أو بتحللها الـ esculin بتحوّله لـ dextrose and
+
+459
+00:34:16,600 --> 00:34:20,580
+esculetin فبتروح تتفاعل هذه المكونات لنتجات مع
+
+460
+00:34:20,580 --> 00:34:24,980
+الفرق iodines أو الفرق ions هذا الفرق موجود بشكل
+
+461
+00:34:24,980 --> 00:34:28,300
+طبيعي في داخل هذا النوع من الـ media تمام بعد هيك
+
+462
+00:34:28,300 --> 00:34:31,520
+بيروح بيتحول للوسط حوالين الـ coolness من dark
+
+463
+00:34:31,520 --> 00:34:34,680
+brown إلى black color وطبعاً هذا النوع من
+
+464
+00:34:34,680 --> 00:34:37,880
+البكتيريا يعاد selective media إلى الـ isolation
+
+465
+00:34:37,880 --> 00:34:41,600
+and identification of this microbe هلا الـ
+
+466
+00:34:41,600 --> 00:34:44,220
+pathogens هي اللي هتكوّنها، حكينا هي موجودة في شكل
+
+467
+00:34:44,220 --> 00:34:47,480
+طبيعي الـ normal flora intestinal tract في حالة الـ
+
+468
+00:34:47,480 --> 00:34:50,320
+abnormal conditions بتعملي pathogens هذه الـ
+
+469
+00:34:50,320 --> 00:34:52,900
+pathogens ممكن تتطور و تصل إلى أنحاء عديدة في
+
+470
+00:34:52,900 --> 00:34:56,800
+الجسم ممكن تصل لـ CNS الـ CNS هو عبارة عن الـ central
+
+471
+00:34:56,800 --> 00:35:00,040
+nervous system و ممكن تصل لـ الـ head لنيك لـ الـ
+
+472
+00:35:00,040 --> 00:35:04,950
+tissues soft tissues لـ الـ skin التيترا عندي طبعاً هي
+
+473
+00:35:04,950 --> 00:35:08,590
+قادرة أو هي أساساً من أحد مكوناتها الـ capsule الـ
+
+474
+00:35:08,590 --> 00:35:11,610
+capsule تبعها يعاد polysaccharides هذا بده يحميها
+
+475
+00:35:11,610 --> 00:35:15,550
+من الفاجوسيتوسس أن تكون بمهاجمتها و كذلك بتعمل
+
+476
+00:35:15,550 --> 00:35:18,430
+على إنتاج الـ abscess formations اللي هي الخراج
+
+477
+00:35:18,430 --> 00:35:24,030
+بالإضافة إلى ذلك بتسبب للبكتيريا في حالة أن وصلت على
+
+478
+00:35:24,030 --> 00:35:29,250
+الدم بشكل غير طبيعي و أيضاً بتسبب لألم في البطن هي
+
+479
+00:35:29,250 --> 00:35:35,320
+الصورة الموضحة عنها و بتسبب لبعض من الدياريالل
+
+480
+00:35:35,320 --> 00:35:37,880
+treatment، العلاج من هذا النوع من البكتيريا، طبعاً
+
+481
+00:35:37,880 --> 00:35:40,980
+عندي الـ antimicrobial متروناغازولز and
+
+482
+00:35:40,980 --> 00:35:45,480
+كلورامفينيكول، بدي أعمل inhibitor للـ produce اللي
+
+483
+00:35:45,480 --> 00:35:48,000
+هي بتنتجها اللي هي الـ beta-lactamase أو الـ beta
+
+484
+00:35:48,000 --> 00:35:52,460
+-lactam، لو روحنا جمعنا بين الـ beta-lactamase
+
+485
+00:35:52,460 --> 00:35:55,980
+inhibitions and الـ beta-lactamase antibiotic، بهذا
+
+486
+00:35:55,980 --> 00:35:59,900
+الطريقة بقدر أنا أمنع من إنتاج هذا النوع عشان ما
+
+487
+00:35:59,900 --> 00:36:02,760
+تسببليش الـ infections الموجودة الـ الـ medical
+
+488
+00:36:02,760 --> 00:36:05,480
+research حكينا بداية أنها بتعمل على تحويل فصائل
+
+489
+00:36:05,480 --> 00:36:11,320
+الدم المختلفة من A أو B أو AB إلى فصيلة O فبالتالي
+
+490
+00:36:11,320 --> 00:36:13,800
+الـ medical راح نستعملها في الـ blood طبعاً هي
+
+491
+00:36:13,800 --> 00:36:16,440
+بتستعمل في مجالات ثانية زي الـ cancer لكن اختصاراً
+
+492
+00:36:16,440 --> 00:36:18,920
+للمقترح هنتحدث فقط عن الـ blood الـ
+
+493
+00:36:18,920 --> 00:36:22,700
+bacteriophagelosis بتعمل على إنتاج إنزيم معين
+
+494
+00:36:22,700 --> 00:36:26,620
+بتقدر فيه تحويل فصيلة الدم بالتحديد الفصيلة O إلى
+
+495
+00:36:26,620 --> 00:36:32,740
+الفصيلة A الفصيلة B إلى الفصيلة O هذه الطريقة بتكون
+
+496
+00:36:32,740 --> 00:36:36,260
+عن طريق تكسير لـ antigens اللي موجودة على الـ
+
+497
+00:36:36,260 --> 00:36:39,980
+surface للـ RBCs الإنزيم اللي بتعمل على إنتاجه هو
+
+498
+00:36:39,980 --> 00:36:43,200
+عبارة عن Alpha Galactosidase لأن أحد مكونات الـ
+
+499
+00:36:43,200 --> 00:36:47,120
+antigen اللي موجود على الـ RBCs اللي خاص بالفصيلة B
+
+500
+00:36:47,120 --> 00:36:52,260
+هو عبارة عن Galactose فإنا لو جبنا له نوع يعمل لي
+
+501
+00:36:52,260 --> 00:36:55,740
+Galactase اللى هو إنزيم يكسر هذا نوع فطريا رح
+
+502
+00:36:55,740 --> 00:36:59,430
+تتحول إلى فصيلة O لأن فصيلة O خالية تماما من أي
+
+503
+00:36:59,430 --> 00:37:02,410
+واحد من الـ antigens هذا بتحدث ليه؟ لإنه طبعا الـ
+
+504
+00:37:02,410 --> 00:37:06,190
+Blood group A بـ O blood group راح يتحدث بشكل عام بيحكي
+
+505
+00:37:06,190 --> 00:37:09,570
+لأن الناس الفاصلة دمهم O عندهم Antigens على الـ
+
+506
+00:37:09,570 --> 00:37:12,810
+RBCs من نوع A الفاصلة دمهم A عندهم Antigens من
+
+507
+00:37:12,810 --> 00:37:16,550
+نوع A والفاصلة دمهم B عندهم Antigens من نوع B
+
+508
+00:37:16,550 --> 00:37:20,250
+والفاصلة دمهم AB عندهم Antigens من نوع A و من
+
+509
+00:37:20,250 --> 00:37:24,500
+نوع B أما الـ O اللي هو الناعي فرقية نوربيه يعني لا
+
+510
+00:37:24,500 --> 00:37:28,640
+عندهم هذا ولا عندهم هذا النوع التكنيك بشكل مختصر و
+
+511
+00:37:28,640 --> 00:37:33,060
+بشكل سريع طبعا بتعملي على إنتاج كما حكينا نوع من
+
+512
+00:37:33,060 --> 00:37:35,840
+أنواع الأنزيمات هو عبارة عن الـ α-galactosidase
+
+513
+00:37:35,840 --> 00:37:39,120
+بيروح بتعملي cut of the sugars in
+
+514
+00:37:39,120 --> 00:37:42,850
+surface of RBCs الخاص في دينالنوع التاني من
+
+515
+00:37:42,850 --> 00:37:46,510
+البكتيريا ما بتصلناش فيه لإنه اقتصرنا للوقت هو عبارة
+
+516
+00:37:46,510 --> 00:37:49,610
+عن الـ Elizabeth King Young Hingospectrum أو الاسم
+
+517
+00:37:49,610 --> 00:37:51,950
+التاني المتعرف عليه أكثر هو عبارة عن
+
+518
+00:37:51,950 --> 00:37:54,610
+الـ Flavobacteria من Hingospectrum النوع التاني من
+
+519
+00:37:54,610 --> 00:37:59,280
+البكتيريا بيحول فصيلة الدم A إلى فصيلة O كيف؟ طبعا
+
+520
+00:37:59,280 --> 00:38:02,880
+نفس الطريقة بتعملي على إنتاج إنزيم خاص اسمه N
+
+521
+00:38:02,880 --> 00:38:06,480
+-acetylgalactamalanase
+
+522
+00:38:06,480 --> 00:38:10,320
+بتحول فصيلة الدم A إلى الفاصلة O بتخلص من الـ
+
+523
+00:38:10,320 --> 00:38:14,240
+sugars الموجودة على الـ surface للـ RBCs و فورا
+
+524
+00:38:14,240 --> 00:38:18,280
+تتحول إلى الفاصلة O هنا طبعا بشكل مختصر حكينا عن
+
+525
+00:38:18,280 --> 00:38:22,940
+البكتيريا Desfragilis بتعملي على إزالة Antigen بيه
+
+526
+00:38:22,940 --> 00:38:25,000
+و بالنسبة للنوع التاني هو عبارة عن الـ Elizabeth
+
+527
+00:38:25,000 --> 00:38:29,340
+King-Yamingospectrum بتتخلص من الفصيلة A طبعا هذا
+
+528
+00:38:29,340 --> 00:38:32,740
+النوع من البكتيريا برضه موجود in normal flora in
+
+529
+00:38:32,740 --> 00:38:35,180
+the intestinal tract و في حالة الـ abnormal
+
+530
+00:38:35,180 --> 00:38:39,540
+conditions بتكون endogenous أو بتسبب لي يعني أمراض
+
+531
+00:38:39,540 --> 00:38:43,210
+و infections قولنا كيف بدنا نستفيد أو إيش السبب من
+
+532
+00:38:43,210 --> 00:38:47,330
+إنّه اكتشفوا هذه الأنزيمات في تحويل فصائل الدم لأن
+
+533
+00:38:47,330 --> 00:38:50,750
+في عندي مثلا أنا مريض فصيلة دم بيه و الفصيلة
+
+534
+00:38:50,750 --> 00:38:53,190
+موجودة عندك في التلاجة مثلا أو المحفوظة عندك
+
+535
+00:38:53,190 --> 00:38:56,690
+فصيلتها أيه طب أنا كيف بدي أوصل أيه لبيه و هذا
+
+536
+00:38:56,690 --> 00:38:59,710
+الشخص في حالة مثلا مرض شديد أو لازم يعني أني
+
+537
+00:38:59,710 --> 00:39:03,570
+أجيب له فصيلة دم و إليه حيموت فورا بستعمل أنزيمات
+
+538
+00:39:03,570 --> 00:39:06,770
+هذا النوع من البكتيريا و بحطها على هذا الدم طبعا
+
+539
+00:39:06,770 --> 00:39:10,310
+مش من شغلنا احنا من شغل التكنولوجيا و بتتحول تلقائيًا
+
+540
+00:39:10,310 --> 00:39:13,210
+فصيلة الدم إلى O متعارفة عليه طبعا هي عبارة عن الـ
+
+541
+00:39:13,210 --> 00:39:17,490
+Universal blood cells يعني أنّها عالمية أنّها بتقدر
+
+542
+00:39:17,490 --> 00:39:22,290
+توزّع لي الدم لجميع الفصائل التالية لأن O بتاعة B و
+
+543
+00:39:22,290 --> 00:39:26,790
+بتاعة A بتاعة AB طبعا فهيك تلقائيًا بتتحول فصيلة
+
+544
+00:39:26,790 --> 00:39:30,550
+الدم إلى O و بقدر أزيتها أنا أسعف هذا المريض فبعطيه
+
+545
+00:39:30,550 --> 00:39:34,850
+من هذا الدم فبالتالي بتكون safety of clinical
+
+546
+00:39:34,850 --> 00:39:37,690
+transfusion شكرا لكم اسمي اسمي
+
+547
+00:39:41,820 --> 00:39:45,020
+طبعا اليوم هنحكي عن بكتيريا مهمة و تم اكتشافها
+
+548
+00:39:45,020 --> 00:39:50,280
+حديثا اسمها الـ  Human bacteria اول
+
+549
+00:39:50,280 --> 00:39:52,960
+شغل قبل ما نتعرف عن خصائص هذه البكتيريا أنّنا نعرف
+
+550
+00:39:52,960 --> 00:39:55,660
+أنّ هذه البكتيريا في عندها ميكانيزم خاص بتقوم فيه
+
+551
+00:39:55,660 --> 00:39:59,100
+اللي هو الـ Bioluminescence اول شيء هذه البكتيريا
+
+552
+00:39:59,100 --> 00:40:05,080
+هذه الخاصية بتقوم ببعث ضوء بتعمل Emission للضوء
+
+553
+00:40:05,080 --> 00:40:08,540
+طبعا تعمل Emission للضوء بوجود إنزيم اسمه الـ
+
+554
+00:40:08,540 --> 00:40:13,900
+Luciferase بحكي إنّ هذه البكتيريا بتكون موجودة
+
+555
+00:40:13,900 --> 00:40:17,820
+بأشياء كثيرة زي الفطريات و طبعا المسكرون بتكون
+
+556
+00:40:17,820 --> 00:40:21,560
+موجودة بالبكتيريا و بتكون موجودة على الـ Gut tract
+
+557
+00:40:21,560 --> 00:40:23,920
+و الـ Skin surface لبعض الـ Marine اللي موجودة في
+
+558
+00:40:23,920 --> 00:40:29,840
+المحيطات و البحار هذه صور بتوضح لنا كيف البكتيريا
+
+559
+00:40:29,840 --> 00:40:30,700
+بتعطي ضوء
+
+560
+00:40:34,050 --> 00:40:37,290
+طبعا هذه البكتيريا منتشرة بشكل واسع زي ما حكينا في
+
+561
+00:40:37,290 --> 00:40:40,190
+الـ Sea water و في الـ Sheens المحيطات و بالـ
+
+562
+00:40:40,190 --> 00:40:44,370
+Freshwater كمان بيقول إنّ هذه البكتيريا قادرة على
+
+563
+00:40:44,370 --> 00:40:48,390
+أنّها تعمل علاقة تكافلية مع بعض الكائنات البحرية زي
+
+564
+00:40:48,390 --> 00:40:52,390
+الـ Fish و الـ Scutes اللي هي السرطانات الـ Crepes و
+
+565
+00:40:52,390 --> 00:40:58,470
+الحبار و الـ Dolphins بعض خصائص البكتيريا أنّ هي
+
+566
+00:40:58,470 --> 00:41:02,610
+Rod shape و كمان Gram negative و كمان هي البكتيريا
+
+567
+00:41:02,610 --> 00:41:09,000
+دموتايوب طبعا نوع الـ Motility flattened motion و طبعا
+
+568
+00:41:09,000 --> 00:41:19,760
+هي Facultative anaerobic هذا
+
+569
+00:41:19,760 --> 00:41:22,020
+الـ Mechanism اللي بتقوم فيه البكتيريا فيه نسط
+
+570
+00:41:22,020 --> 00:41:26,940
+بسترات اسمها الـ Luciferin بوجود الأكسجين بإنزيم
+
+571
+00:41:26,940 --> 00:41:34,180
+اسمه الـ Luciferase بيعطينا الـ Light هذه الميكانيزم
+
+572
+00:41:34,180 --> 00:41:37,340
+تبعتها طبعا بيحكي لأن كل بكتيريا .. هذه البكتيريا
+
+573
+00:41:37,340 --> 00:41:41,620
+عندها قدرة على إنتاج الـ .. الـ Auto-inducer كل
+
+574
+00:41:41,620 --> 00:41:44,560
+بكتيريا بتعطيني Auto-inducer طبعا الـ Auto-inducer
+
+575
+00:41:44,560 --> 00:41:48,940
+هذا احنا لما أخذنا خاصية الـ Biofilm في البكتيريا
+
+576
+00:41:48,940 --> 00:41:52,660
+عندها القدرة على عمل Quorum sensing فكل بكتيريا
+
+577
+00:41:52,660 --> 00:41:55,760
+بتعطيني Auto-inducer واحد واللي بنظم هذه العملية
+
+578
+00:41:55,760 --> 00:42:01,980
+في عندنا Gene اسمه Lux operon Gene طبعا بيحكي لي الـ
+
+579
+00:42:01,980 --> 00:42:05,820
+Substrate بتاعة البكتيريا هذه في عندنا اللي هي
+
+580
+00:42:05,820 --> 00:42:10,780
+Flavin mononucleotide مع الـ O2 و بوجود الـ
+
+581
+00:42:10,780 --> 00:42:15,520
+Long chain من الـ Fatty aldehyde طبعا بالنهاية
+
+582
+00:42:15,520 --> 00:42:18,860
+بيعطيني طاقة و بيعطيني ضوء و طبعا الضوء اللي
+
+583
+00:42:18,860 --> 00:42:22,680
+بتعطيني هذه البكتيريا اللي هو Blue green و ممكن
+
+584
+00:42:22,680 --> 00:42:29,620
+نشوفه على طول موج 490 nanometer هذا الكلام حكيناه
+
+585
+00:42:29,620 --> 00:42:34,020
+طوال طبعا الأصناف تبعت هذه البكتيريا ثلاث أصناف تم
+
+586
+00:42:34,020 --> 00:42:37,260
+اكتشافها اللي هي الـ Vibrio و الـ Photobacterium و الـ 
+
+587
+00:42:37,260 --> 00:42:42,340
+Photobacterium طبعا الـ Vibrio في منها
+
+588
+00:42:42,340 --> 00:42:45,760
+نوعين الـ Fischeri و الـ .. و الـ Harveyi
+
+589
+00:42:48,270 --> 00:42:51,050
+الميديا اللي بنستخدمها عشان ننمي هذه البكتيريا
+
+590
+00:42:51,050 --> 00:42:53,310
+الميديا مرت علينا الـ Thiosulfate Citrate
+
+591
+00:42:53,310 --> 00:42:57,550
+Bile salts Sucrose الـ TCPC-Agar و الميديا التانية الـ
+
+592
+00:42:57,550 --> 00:43:00,930
+Modified Sea Water Complete و طبعا بتاعتها لون أخضر
+
+593
+00:43:00,930 --> 00:43:06,270
+و أزرق على الميديا هاي صورة للميديا بنمي عليها
+
+594
+00:43:06,270 --> 00:43:10,510
+البكتيريا و هاي الألوان زي ما بتشوفين مش واضح
+
+595
+00:43:10,510 --> 00:43:13,290
+كثير أزرق و أخضر بيكون اللون
+
+596
+00:43:15,630 --> 00:43:18,530
+بعض التطبيقات لهذه البكتيريا بيقول إنهم استخدموها
+
+597
+00:43:18,530 --> 00:43:22,150
+في مجال الـ Food microbiology كمان استخدموها كـ
+
+598
+00:43:22,150 --> 00:43:25,230
+Biotechnology و استخدموها في الـ Drug diagnosis و
+
+599
+00:43:25,230 --> 00:43:29,050
+كمان هذه البكتيريا بيقول إنّها بتعمل تقليل على الـ
+
+600
+00:43:29,050 --> 00:43:34,390
+Toxicity للـ Water و كمان تم اكتشافها في .. طبعا هذه
+
+601
+00:43:34,390 --> 00:43:38,090
+البكتيريا بتكشف عن وجود الـ Fossil fuels اللي هو
+
+602
+00:43:38,090 --> 00:43:41,670
+الوقود الأحفوري و بتستخدم في الـ Cold war اللي هي
+
+603
+00:43:41,670 --> 00:43:45,920
+الحرب الباردة طبعا هذه من مساوئها و كمان بيقول لي في
+
+604
+00:43:45,920 --> 00:43:48,720
+دراسة حديثة بتحكي أنّهم استخدموا هذه البكتيريا في
+
+605
+00:43:48,720 --> 00:43:52,340
+إنارة الطرق و الشوارع و يعني هذا تطبيق جيد لو احنا
+
+606
+00:43:52,340 --> 00:43:56,040
+استخدمناه و في دراسات كثيرة تمت على هذه البكتيريا و
+
+607
+00:43:56,040 --> 00:43:59,480
+إن شاء الله بيتم هنا دراسة بغزة و بيلقوا في البحر
+
+608
+00:43:59,480 --> 00:44:02,620
+تبعنا و بيستخلصوا البكتيريا و بنقدر إنّه نضئ
+
+609
+00:44:02,620 --> 00:44:05,200
+الشوارع بدون .. وش كده؟
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/5rnHqFi4ziI_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/5rnHqFi4ziI_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..6de99741f8bb5ac2612ec61843440eb73ff7b08b
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/5rnHqFi4ziI_raw.json
@@ -0,0 +1 @@
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عارفين انه الانتقال في كالقول و الاحتكاك المباشر مع المرضى قد يعني انت عارفين given aseptic technique لو راح تخيلها عملية معقدة جدا", "tokens": [3615, 8592, 7649, 1975, 5016, 8315, 6225, 9640, 5172, 9957, 16472, 3224, 2423, 7649, 2655, 4587, 6027, 8978, 9122, 6027, 39648, 4032, 42963, 33753, 4117, 995, 4117, 9673, 3555, 33599, 2288, 20449, 9673, 43042, 7578, 12174, 3215, 37495, 22653, 16472, 2655, 6225, 9640, 5172, 9957, 2212, 382, 5250, 299, 6532, 45164, 12602, 39319, 6055, 9778, 26895, 11296, 6225, 42213, 10632, 20449, 28543, 3660, 10874, 28259], "avg_logprob": -0.17660984713019748, "compression_ratio": 1.5290322580645161, "no_speech_prob": 2.384185791015625e-07, "words": [{"start": 1049.86, "end": 1050.34, "word": "عشان", "probability": 0.9361979166666666}, {"start": 1050.34, "end": 1050.5, "word": " احنا", "probability": 0.8917643229166666}, {"start": 1050.5, "end": 1050.94, "word": " عارفين", "probability": 0.97705078125}, {"start": 1050.94, "end": 1051.46, "word": " انه", 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"word": " و", "probability": 0.94873046875}, {"start": 2633.36, "end": 2633.5, "word": " في", "probability": 0.74853515625}, {"start": 2633.5, "end": 2633.94, "word": " دراسات", "probability": 0.9256591796875}, {"start": 2633.94, "end": 2634.24, "word": " كتير", "probability": 0.9609375}, {"start": 2634.24, "end": 2634.52, "word": " تمت", "probability": 0.989013671875}, {"start": 2634.52, "end": 2634.66, "word": " على", "probability": 0.85888671875}, {"start": 2634.66, "end": 2634.88, "word": " هذه", "probability": 0.97802734375}, {"start": 2634.88, "end": 2635.54, "word": " البكتيريا", "probability": 0.985546875}, {"start": 2635.54, "end": 2636.04, "word": " و", "probability": 0.99365234375}, {"start": 2636.04, "end": 2636.34, "word": " ان", "probability": 0.60693359375}, {"start": 2636.34, "end": 2636.52, "word": " شاء", "probability": 0.959228515625}, {"start": 2636.52, "end": 2636.58, "word": " الله", "probability": 0.95556640625}, {"start": 2636.58, "end": 2636.9, "word": " بيتم", "probability": 0.76611328125}, {"start": 2636.9, "end": 2637.12, "word": " هنا", "probability": 0.8740234375}, {"start": 2637.12, "end": 2637.46, "word": " دراسة", "probability": 0.994140625}, {"start": 2637.46, "end": 2637.8, "word": " بغزة", "probability": 0.81793212890625}, {"start": 2637.8, "end": 2637.92, "word": " و", "probability": 0.94482421875}, {"start": 2637.92, "end": 2638.56, "word": " بيلقوا", "probability": 0.832763671875}, {"start": 2638.56, "end": 2638.88, "word": " في", "probability": 0.927734375}, {"start": 2638.88, "end": 2639.48, "word": " البحر", "probability": 0.9939778645833334}, {"start": 2639.48, "end": 2639.92, "word": " تبعنا", "probability": 0.9678955078125}, {"start": 2639.92, "end": 2640.34, "word": " و", "probability": 0.984375}, {"start": 2640.34, "end": 2640.98, "word": " بيستخلصوا", "probability": 0.9868861607142857}, {"start": 2640.98, "end": 2641.52, "word": " البكتيريا", "probability": 0.9205078125}, {"start": 2641.52, "end": 2641.82, "word": " و", "probability": 0.99658203125}, {"start": 2641.82, "end": 2642.12, "word": " بنقدر", "probability": 0.9148763020833334}, {"start": 2642.12, "end": 2642.34, "word": " إنه", "probability": 0.657470703125}, {"start": 2642.34, "end": 2642.62, "word": " نضيق", "probability": 0.892333984375}, {"start": 2642.62, "end": 2643.16, "word": " الشوارع", "probability": 0.9920654296875}, {"start": 2643.16, "end": 2643.6, "word": " بدون", "probability": 0.996826171875}, {"start": 2643.6, "end": 2644.36, "word": " ..", "probability": 0.6611328125}, {"start": 2644.36, "end": 2644.86, "word": " وش", "probability": 0.73486328125}, {"start": 2644.86, "end": 2645.2, "word": " كده؟", "probability": 0.74456787109375}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 2646.37825, "duration_after_vad": 2463.272499999992}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/5rnHqFi4ziI_raw.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/5rnHqFi4ziI_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..af2b95ef65cf1a0d831528fd07c2aafe9850f3ae
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/5rnHqFi4ziI_raw.srt
@@ -0,0 +1,2436 @@
+1
+00:00:20,670 --> 00:00:22,550
+بسم الله الرحمن الرحيم انا هتكلم اليوم موضوع جديد
+
+2
+00:00:22,550 --> 00:00:26,330
+ان شاء الله بعنوان bacterial vaccines هو الأنوان
+
+3
+00:00:26,330 --> 00:00:30,850
+جديد لكن انا متأكد المعلومات اللي هنشوفها ضمن هذا
+
+4
+00:00:30,850 --> 00:00:36,110
+ال chapter هتلاقي ان جزء الكبير منها تم دراسته كنا
+
+5
+00:00:36,110 --> 00:00:39,190
+بيعرف على سبيل المثال انه من الميكروبات اللي
+
+6
+00:00:39,190 --> 00:00:42,770
+دراسناهم Salmonella كنت prevented by ال vaccine
+
+7
+00:00:42,770 --> 00:00:47,860
+Shigella كنت prevented by ال vaccineكثير من
+
+8
+00:00:47,860 --> 00:00:50,300
+الأمراض اللي اضطررتنا إليها قلنا vaccine
+
+9
+00:00:50,300 --> 00:00:53,400
+preventable disease قلتلي هل أنا بعرفها في بعض
+
+10
+00:00:53,400 --> 00:00:57,820
+الأمراض هذه اليوم هحنحاول إنه نعمل categorization
+
+11
+00:00:57,820 --> 00:01:02,480
+عملية تصنيف لل vaccine و تعريف قلنا ال process
+
+12
+00:01:02,480 --> 00:01:06,710
+immunizationاللي هم نقولها دعاها بالتطاين او
+
+13
+00:01:06,710 --> 00:01:10,450
+التوقيع could be active و could be passive يعني
+
+14
+00:01:10,450 --> 00:01:14,970
+ممكن نعطي انتجينات و ممكن نعطي antibodies إذا
+
+15
+00:01:14,970 --> 00:01:18,930
+أعطينا انتجينات الجسم بيكون antibodies و أنا بسميه
+
+16
+00:01:18,930 --> 00:01:23,590
+active immunization إذا أعطينا ال universe جهزة
+
+17
+00:01:23,590 --> 00:01:27,870
+برا بيسميها passive immunization و الحالتين
+
+18
+00:01:27,870 --> 00:01:32,110
+مطموضين إنه نعمل active و نعمل passiveناخد أول
+
+19
+00:01:32,110 --> 00:01:36,130
+مثال أكتر immunity is induced by vaccines prepared
+
+20
+00:01:36,130 --> 00:01:39,070
+from bacteria or their products يبقى ال vaccines
+
+21
+00:01:39,070 --> 00:01:44,930
+في اللوحة هذه بتقول كلمتين مهمين إما ال vaccine هي
+
+22
+00:01:44,930 --> 00:01:48,710
+عبارة عن إما البكتيريا نفسها أو منتجاتها
+
+23
+00:01:48,710 --> 00:01:53,810
+والبكتيريا إما killed or attenuated يعني إما ميتة
+
+24
+00:01:53,810 --> 00:02:00,320
+أو مضاعفةأو منتجاتها ايش هنا منتجاتها؟ ممكن
+
+25
+00:02:00,320 --> 00:02:05,100
+proteins ممكن fragments من ال proteins او من
+
+26
+00:02:05,100 --> 00:02:09,300
+الخلية إذا في الآخر مش الخلية كلها و ده أجزاء من
+
+27
+00:02:09,300 --> 00:02:13,020
+الخلية ال passive زي ما حكينا it is preformed
+
+28
+00:02:13,020 --> 00:02:17,460
+antibodies يعني أجسام مضادة مجهزة في الخارج قاطعة
+
+29
+00:02:17,460 --> 00:02:22,040
+للمريض لحمايته من مرض معين أو يبادله من مرض معين
+
+30
+00:02:23,030 --> 00:02:26,950
+رقم تلاتة ليلة غريبة active and passive at the
+
+31
+00:02:26,950 --> 00:02:31,610
+same time بنعطي vaccine وبنعطي immunoglobulin وفي
+
+32
+00:02:31,610 --> 00:02:35,490
+مثلان الصحيح في الطب مشهورين جدا في هذا المجال
+
+33
+00:02:35,490 --> 00:02:41,670
+نبدأ فيهم انت منهم تلعب تيتا نص؟ تيتا نص و ال
+
+34
+00:02:41,670 --> 00:02:44,710
+rabies ال rabies يمكن يكون أسهل للشرح خلنا نشرحها
+
+35
+00:02:44,710 --> 00:02:49,120
+ال rabies ال rabies هو يبقى عبارة عن دا الكلاباللي
+
+36
+00:02:49,120 --> 00:02:53,980
+لو عض .. كلمة عض يعني .. لأنه طريقة الانتقال عن
+
+37
+00:02:53,980 --> 00:02:57,560
+طريق الكلاب اتضالة و الغالب اللي هم صورة فبعض
+
+38
+00:02:57,560 --> 00:03:01,020
+البلدان لإن الكلاب تم تطيمها و خلصنا من الكلاب
+
+39
+00:03:01,020 --> 00:03:04,040
+اتضالة فصار الانتقال عن طريق أشياء أخرى زي
+
+40
+00:03:04,040 --> 00:03:07,380
+الخفافيش على سبيل المثال و عن طريق السناجب ..
+
+41
+00:03:07,380 --> 00:03:09,720
+بتعرف السناجب الحلوين هدولة؟ هدولة اللي ممكن
+
+42
+00:03:09,720 --> 00:03:13,380
+تنقلونها ال .. في حيوانات كثيرة ممكن تنقل اللي هو
+
+43
+00:03:13,380 --> 00:03:18,520
+ال rapistال animal بيسموه rabid animal، rabid
+
+44
+00:03:18,520 --> 00:03:22,900
+animal يعني حيوان عنده مرض اللي هو rabies، rabid
+
+45
+00:03:22,900 --> 00:03:28,480
+animal ال rabid animal لو عرض إنسان، فالأن في
+
+46
+00:03:28,480 --> 00:03:33,200
+احتمالية ان هذا الشخص صار .. لأن هو exposed، صار
+
+47
+00:03:33,200 --> 00:03:37,680
+ايش انه exposed؟ تعرض، فالحاجة الأحسن اللي احنا
+
+48
+00:03:37,680 --> 00:03:41,380
+نعطيه، نعطيه vaccine و نعطيه antibodies، هو تعرض
+
+49
+00:03:41,380 --> 00:03:46,830
+alreadyمظبوط نعطيها antibodies لكن لإنه .. لإنه ال
+
+50
+00:03:46,830 --> 00:03:51,470
+virus اللي هو رحلة من الجلد إلى ال central nervous
+
+51
+00:03:51,470 --> 00:03:55,610
+system بتاخد أيام هذه الفترة الزمنية .. هذه الفترة
+
+52
+00:03:55,610 --> 00:04:02,190
+الزمنية كافية إنه أنا أشتغل بالطريقتينبقى احكم
+
+53
+00:04:02,190 --> 00:04:07,070
+المكان المصاب ب immunoglobulins immunoglobulins هل
+
+54
+00:04:07,070 --> 00:04:10,930
+بيقبل neutralization؟ لأ اللي هو ال anti .. ال
+
+55
+00:04:10,930 --> 00:04:17,190
+vaccine .. ال virus .. ال virus نفسه و على الجهة
+
+56
+00:04:17,190 --> 00:04:21,930
+التانية مثلا اللي هو العضة في رجله الشمال تمام؟
+
+57
+00:04:21,930 --> 00:04:25,680
+بروح على أيديه اليمين و بعطيه vaccineليش بعضهم مش
+
+58
+00:04:25,680 --> 00:04:28,800
+في نفس المكان؟ عشان ال vaccine إيش هو؟ Antigen
+
+59
+00:04:28,800 --> 00:04:31,580
+وهذا اللي حطيناها بالأول Antibody، لو التقل
+
+60
+00:04:31,580 --> 00:04:34,100
+Antibody مع ال Antigen هي Value Neutralization، صح
+
+61
+00:04:34,100 --> 00:04:37,580
+ولا لأ؟ فبعطي ال vaccine في جهة و بعطي ال
+
+62
+00:04:37,580 --> 00:04:40,520
+immunoglobulin في جهة تانية كمان، ليش عطينا
+
+63
+00:04:40,520 --> 00:04:44,480
+vaccine في هذه الحالة؟ لإن في فترة زمنية طويلة ما
+
+64
+00:04:44,480 --> 00:04:48,680
+بين ال site of entry لإنه يصل ل center of impulse
+
+65
+00:04:48,680 --> 00:04:52,960
+system ففي فرصة إن الاسم يكون أسام مضادةالى هاي
+
+66
+00:04:52,960 --> 00:04:55,280
+مثلها ال active passive immunization في نفس اللي
+
+67
+00:04:55,280 --> 00:04:58,160
+هو الوقت لكن في غير بالأمر ايه هتعطيه منه بيبولين
+
+68
+00:04:58,160 --> 00:05:03,500
+ايه هتعطيه فاكسينه ده على حساب الحلالـ Active
+
+69
+00:05:03,500 --> 00:05:06,780
+Immunity ممكن تقسم الفاكسين إلى تلات أنواع أو تلات
+
+70
+00:05:06,780 --> 00:05:09,720
+أصحاب من الفاكسين الصندوق الأول الـ capsular
+
+71
+00:05:09,720 --> 00:05:12,580
+polysaccharide فستجدوا حالكوا بتاعه فوق كويس
+
+72
+00:05:12,580 --> 00:05:16,000
+Inactivated Protein Toxins اللي هي الـ Toxites
+
+73
+00:05:16,000 --> 00:05:20,540
+وبتاعه فوق وضبط كويس تلاتة killed bacteria or live
+
+74
+00:05:20,540 --> 00:05:23,540
+attenuated bacteria كلمة live يعني
+
+75
+00:05:29,820 --> 00:05:34,280
+بالنسبة للمعالجات المطلوبة، لكن البكتيريا اتعرضت
+
+76
+00:05:34,280 --> 00:05:38,340
+له معالجات جعلتها غير قادرة على إحداث طعام
+
+77
+00:05:38,340 --> 00:05:41,620
+الgenesis وبالتالي هي attenuated قرّى لزمى ال
+
+78
+00:05:41,620 --> 00:05:44,820
+infection، لكن غير قادرة على إتحاد ال disease
+
+79
+00:05:44,820 --> 00:05:50,810
+وبالتالي المريض بيحتك بمجموعة من البكتيرياو
+
+80
+00:05:50,810 --> 00:05:55,490
+بيقتلها معناه حاكيش انه عنده experience، كلمة
+
+81
+00:05:55,490 --> 00:05:58,710
+experience يعني عنده memory و عنده antibodies لو
+
+82
+00:05:58,710 --> 00:06:01,790
+البكتيريا يخلق مرة أخرى بطريقة natural، بيقدر
+
+83
+00:06:01,790 --> 00:06:04,570
+يقاوم، إذن captured by polysaccharide، inactivated
+
+84
+00:06:04,570 --> 00:06:08,790
+proteins تكسوك، تلقى a killed bacteria or live
+
+85
+00:06:08,790 --> 00:06:16,060
+attenuated bacteriaنجوم الـ meningitis فاكرينهم؟
+
+86
+00:06:16,060 --> 00:06:19,620
+هم نفسهم النجوم اللي يلهم الكابسول صح ولا لا؟
+
+87
+00:06:19,620 --> 00:06:23,540
+وبالتالي كون الكابسول is the major the main
+
+88
+00:06:23,540 --> 00:06:26,660
+violence factor of this organism يبقى المتوقع ان
+
+89
+00:06:26,660 --> 00:06:30,960
+ال vaccine هو هيستهدف أيضا اللي هو الكابسول فأول
+
+90
+00:06:30,960 --> 00:06:35,520
+واحدة استبتت كوكاس ميمونيا vaccine وأول صحية ينتمي
+
+91
+00:06:35,520 --> 00:06:39,400
+إلى صنفين في السوق موجود منه vaccines واحد بحتوى
+
+92
+00:06:39,400 --> 00:06:43,880
+على 23 serotypesفاكريني الوقت قبل ما اشرفتش؟ تلاتة
+
+93
+00:06:43,880 --> 00:06:46,640
+و تمانين؟ اربعة و تمانين اتكلمت عنها على طول خلاص
+
+94
+00:06:46,640 --> 00:06:50,680
+اربعة و تمانين .. اربعة و تمانين stereotypes بس
+
+95
+00:06:50,680 --> 00:06:54,160
+احنا لازم نجد ايش منهم؟ تلاتة و عشرين هذا ايش
+
+96
+00:06:54,160 --> 00:06:57,740
+واصفه تلاتة و عشرين؟ the most common the most
+
+97
+00:06:57,740 --> 00:07:01,900
+prevalent الاكتر شيوعا في المنطق ده مين اللي
+
+98
+00:07:01,900 --> 00:07:06,020
+بيعطوا هدوله؟ بيعطوا لناس اللي فوق الستين سنة
+
+99
+00:07:06,020 --> 00:07:11,450
+تمام؟ اللي هم normal ماعنفش اي مشكلةللي أعمارهم
+
+100
+00:07:11,450 --> 00:07:14,990
+أقل من ستين بس بيمتلكوا واحد أو أكتر من هذه الشروط
+
+101
+00:07:14,990 --> 00:07:20,730
+اللي احنا راح نتكلم عنها مثلا عنده diabetes، عنده
+
+102
+00:07:20,730 --> 00:07:25,110
+serosis، تلايف، عنده compromised spleen function،
+
+103
+00:07:25,110 --> 00:07:27,810
+يعني عنده مشكلة في وظيفة الـspleen، أو عنده
+
+104
+00:07:27,810 --> 00:07:31,350
+sphenectomy، يعني الـspleen تم استئثاره، تم
+
+105
+00:07:31,350 --> 00:07:35,390
+استئثاره وبطوحاه، تم استئثاره، هدول الناس لأ، ايه
+
+106
+00:07:35,390 --> 00:07:39,590
+ربما هم بياخدوا اللي هو ال vaccineالنوع التاني من
+
+107
+00:07:39,590 --> 00:07:43,530
+ال vaccines بيحتوي على عدد أقل بيحتوي على عدد أقل
+
+108
+00:07:43,530 --> 00:07:48,610
+وهم سبعة بس خدوا باركم it is coupled with a
+
+109
+00:07:48,610 --> 00:07:50,790
+carrier protein ايش يعني coupled with a carrier
+
+110
+00:07:50,790 --> 00:07:53,770
+protein؟ انا بقول دالما انه
+
+111
+00:07:56,090 --> 00:08:00,850
+لو هذا ال antigen هو ال protein بالمناسبة او ال
+
+112
+00:08:00,850 --> 00:08:02,770
+polysaccharide و ال polysaccharide او ال protein
+
+113
+00:08:02,770 --> 00:08:07,090
+they are antigenic لكن لأن الحجم matters يعني بيهم
+
+114
+00:08:07,090 --> 00:08:10,850
+الحجم في ال antigenات زي ما تتدرسوا في المناعة
+
+115
+00:08:10,850 --> 00:08:15,410
+ابتراحوا ان لو ركّبت انا هذا على carrier protein
+
+116
+00:08:15,410 --> 00:08:19,950
+بصير حجمه اكبر صحيح هذا مابش اي قيمة من ناحية ال
+
+117
+00:08:19,950 --> 00:08:24,210
+antigenات لكن القيمة لمين؟بالانتجين نفسه، لكن
+
+118
+00:08:24,210 --> 00:08:29,170
+كموليكيل كله كبر، فصوت ال immunogenicity بعته أعلى
+
+119
+00:08:29,170 --> 00:08:32,950
+وهذه عملية معروفة ومشهورة بنسمي المركب إما
+
+120
+00:08:32,950 --> 00:08:38,010
+conjugate أو adjuvant اللي هو مادة محفزة على ال
+
+121
+00:08:38,010 --> 00:08:41,110
+antigenicity نفهم الحالة على فكرة نستخدم ال
+
+122
+00:08:41,110 --> 00:08:44,730
+diphtheria toxoid كadjuvant أو كconjugate يعني
+
+123
+00:08:44,730 --> 00:08:49,130
+بنركب المركب اللي بنيها رام عالمين على diphtheria
+
+124
+00:08:49,130 --> 00:08:53,580
+toxoidيبقى الأولاني ال conjugated والثاني
+
+125
+00:08:53,580 --> 00:08:58,320
+conjugated أكم واحد الأولاني بيحتوي ايه؟ التاني
+
+126
+00:08:58,320 --> 00:09:05,860
+لمن يبقى في التاني لأن أطفال اللي لم يستجيبوا بشكل
+
+127
+00:09:05,860 --> 00:09:10,800
+جيد لل vaccine الأولاني يبقى بتوقع أن ال vaccine
+
+128
+00:09:10,800 --> 00:09:15,740
+التاني الاستجابة اللي هو أفضل ليش أفضل؟ لأنه كما
+
+129
+00:09:15,740 --> 00:09:19,340
+حكينا conjugated with diphtheria
+
+130
+00:09:23,030 --> 00:09:27,230
+في مصطلح بنا يستخدمه الآن هنفهمه كويس قدر نركز مع
+
+131
+00:09:27,230 --> 00:09:33,110
+بعض و بدون كتاب المراجع انتوا اربعة و تمانين اربعة
+
+132
+00:09:33,110 --> 00:09:37,170
+و تمانين لما انا ماسألش او ماعطيش محاضرة او
+
+133
+00:09:37,170 --> 00:09:43,010
+مااهتمش الا بتلاتة و عشرين تمام؟ او بلاش بتلاتة و
+
+134
+00:09:43,010 --> 00:09:47,830
+عشرين صار مضمونين مين اللي مش مضمونين؟ المبنيين
+
+135
+00:09:48,430 --> 00:09:53,470
+بطلنا نهتم بالـ23 فبقعدنا نهتمنا فيهم يردبوا و
+
+136
+00:09:53,470 --> 00:09:58,830
+يدوبوا و يخلصوا من المجتمع لكن الباقي موجودين
+
+137
+00:09:58,830 --> 00:10:03,290
+صحيح؟ يعني اللي لم نقتلهم بال vaccine وبالتالي
+
+138
+00:10:03,290 --> 00:10:07,470
+بنخلق من ما يسمى بـ Serotype Replacement ان اللي
+
+139
+00:10:07,470 --> 00:10:12,270
+مااهتملاش فيهم بال vaccine يحلوا محل اللي اهتمنا
+
+140
+00:10:12,270 --> 00:10:16,330
+فيهم بال vaccine واضحة ممكن و مش واضحة ان اوضحها
+
+141
+00:10:16,330 --> 00:10:21,850
+كمان مرةعندي 23 نوع او سلالة من البكتيريا كوضع لهم
+
+142
+00:10:21,850 --> 00:10:24,850
+فاكسين هدولة هارفوا البلد كلهم مالوجلهم مش سوء او
+
+143
+00:10:24,850 --> 00:10:29,410
+مكان يقعدوا فيه شهر perfection صح؟ ايش مصيرهم؟ الى
+
+144
+00:10:29,410 --> 00:10:36,130
+زوالة مين اللي بيضاروا؟ الباجين اللي مالهمش
+
+145
+00:10:36,130 --> 00:10:40,970
+antivirus في أجسامنا ممكن اي من هؤلاء يحلوا محل
+
+146
+00:10:40,970 --> 00:10:45,430
+الواحد من التلاتة وعشرين بظاهرة نسميها Serotype
+
+147
+00:10:45,430 --> 00:10:48,750
+Replacement نطمنكمفي السطر الأخير أو الليلة
+
+148
+00:10:48,750 --> 00:10:53,870
+الأخيرة من اللوحة بتقول التالت إن هذا التخوف اللي
+
+149
+00:10:53,870 --> 00:10:58,070
+هو Zero Type Replacement هو تخوف نظري ولم نجد أي
+
+150
+00:10:58,070 --> 00:11:02,670
+بيانات لحد الآن تدعم هذا التخوف النظري لكن هي
+
+151
+00:11:02,670 --> 00:11:08,010
+ظاهرة منطقية .. منطقية متوقعة وما زالت to be
+
+152
+00:11:08,010 --> 00:11:11,870
+evaluated يعني باستمرار الأرواح ده بتطلعوا على هل
+
+153
+00:11:11,870 --> 00:11:16,050
+صار انتشار لسوريات جديدة غير ال 23 أو غير السبعة
+
+154
+00:11:16,050 --> 00:11:19,040
+اللي احنا استخدمناهم أو لالكن لحد ما مافيش بيانات
+
+155
+00:11:19,040 --> 00:11:25,140
+تشير إلى انه ظاهرة مهمة و بتاعة نعترف من ال
+
+156
+00:11:25,140 --> 00:11:28,660
+capsular polysaccharide neisseria meningitidis
+
+157
+00:11:29,610 --> 00:11:35,310
+ناسيريا meningitis احنا اتكلمنا عنها و كنا بتعمل
+
+158
+00:11:35,310 --> 00:11:38,570
+explosive .. explosive يعني مفجري .. explosive
+
+159
+00:11:38,570 --> 00:11:45,130
+epidemics و ذكرنا بعض النقاط الحساسة اللي وين ممكن
+
+160
+00:11:45,130 --> 00:11:49,250
+تنتشر فيها من ضمن اننا كنا college students طلاب
+
+161
+00:11:49,250 --> 00:11:53,830
+الجامعة اللي بيعيشوا في سكن داخلي و المجننين الجدر
+
+162
+00:11:53,830 --> 00:11:59,340
+و هتلاحظوا ان اكتر فئات لابنقصح إننا نعطيهم ال
+
+163
+00:11:59,340 --> 00:12:02,340
+vaccine، هم الفئتين اللي ذكرتهم الآن، احنا كمسلمين
+
+164
+00:12:02,340 --> 00:12:06,520
+لخاصية جمهنين .. مجتمع الو السودان .. مجتمع الو
+
+165
+00:12:06,520 --> 00:12:12,200
+السودان و احنا كمسلمين النيزة ليه الحج، إنه قبل
+
+166
+00:12:12,200 --> 00:12:16,750
+الحج بناخد هذا أيضا ال vaccineاللي بيحتوي
+
+167
+00:12:16,750 --> 00:12:20,310
+الفاكسينة على أربع cellotypes من الكبسول ولازم
+
+168
+00:12:20,310 --> 00:12:28,950
+نحفظهم الآن ACW سهلين ACW 139 والأخيرة Y ACW 135
+
+169
+00:12:28,950 --> 00:12:32,330
+اللي هو Y هلأ الأربعة اللي موجودة برضه ليه منه
+
+170
+00:12:32,330 --> 00:12:38,310
+شكلين شكل الأول conjugated والتاني non conjugated
+
+171
+00:12:38,310 --> 00:12:39,570
+لإن اللي بيحتوي على ال
+
+172
+00:12:44,820 --> 00:12:47,460
+it is given when there is a high risk of
+
+173
+00:12:47,460 --> 00:12:51,280
+meningitis when
+
+174
+00:12:51,280 --> 00:12:56,900
+students enter college and are living in dormitory
+
+175
+00:12:56,900 --> 00:13:01,640
+when military recruits enter boot camp اللي هو
+
+176
+00:13:01,640 --> 00:13:05,000
+المعسكرات التدريب والتجنيد or for travelers
+
+177
+00:13:06,390 --> 00:13:08,590
+المسافرين اللي احنا من ضمنهم الحجار اللي بيبقى
+
+178
+00:13:08,590 --> 00:13:12,310
+مسافرين to areas where meningitis الان في مصطلح
+
+179
+00:13:12,310 --> 00:13:16,930
+اول مرضاه هنا المحاضر الماضي مر او اللي جابنا كلمة
+
+180
+00:13:16,930 --> 00:13:21,750
+endemic endemic ميزنها عن epidemic المرض هو انه
+
+181
+00:13:21,750 --> 00:13:25,950
+hyper endemic wherein a disease incidence is
+
+182
+00:13:25,950 --> 00:13:30,790
+constantly high in one area يعني نسبة حدوث المرض
+
+183
+00:13:30,790 --> 00:13:32,210
+عالية في
+
+184
+00:13:35,630 --> 00:13:40,690
+نقول نسبة الإصابة سنوية مثلا 20 ألف إصابة لكل 100
+
+185
+00:13:40,690 --> 00:13:44,450
+ألف شخص هذه نسبة عالية جدا لكن سنويا بتحدث هذي
+
+186
+00:13:44,450 --> 00:13:49,150
+بيسموها hyper endemic hyper endemic يعني مستوطن
+
+187
+00:13:49,150 --> 00:13:53,490
+المرض و يحدث بنسبة عالية النوع التالت بره من نجوم
+
+188
+00:13:53,490 --> 00:13:58,490
+ال meningitis Hemophilus influenza و من ميزاتها ان
+
+189
+00:13:58,490 --> 00:14:02,890
+ال capsule تبعتها is poorly immunogenic
+
+190
+00:14:05,990 --> 00:14:11,930
+لو حطيناها لحالها عند الإنسان للأسف بيكونش الإنسان
+
+191
+00:14:11,930 --> 00:14:15,970
+إلها أجسام مضادة بكميات كبيرة، لذا مافيش خيار
+
+192
+00:14:15,970 --> 00:14:20,010
+قدامها لأنه يكون conjugated و unconjugated، لازم
+
+193
+00:14:20,010 --> 00:14:25,310
+يكون مفلوس influenza vaccine contains the type B
+
+194
+00:14:25,310 --> 00:14:29,790
+polysaccharideالـ type B polysaccharide يبقى فيه
+
+195
+00:14:29,790 --> 00:14:34,210
+لنا strand واحد conjugated to diphtheria toxoid or
+
+196
+00:14:34,210 --> 00:14:39,130
+other carrier proteins يبقى فيه لنا conjugation
+
+197
+00:14:39,130 --> 00:14:42,510
+لإما diphtheria اللي هي اللي conjugated أو other
+
+198
+00:14:42,510 --> 00:14:46,130
+proteins it is given to children between the ages
+
+199
+00:14:46,130 --> 00:14:47,950
+of two to fifteen months
+
+200
+00:14:51,880 --> 00:14:56,700
+و قلتلكوا إن العمر حتى جدول ال vaccines في العالم
+
+201
+00:14:56,700 --> 00:15:02,180
+بتغير كل ما بتتراكم لنا بيانات جديدة بتحسن من
+
+202
+00:15:02,180 --> 00:15:05,820
+فهمنا لبعض الأمور ممكن نغيّر في جناح ال
+
+203
+00:15:05,820 --> 00:15:12,480
+vaccination فلما أنا أقول البداية من شهرين لحد 15
+
+204
+00:15:12,480 --> 00:15:15,380
+شهر يمكن هذه الفترة اللي بتسبق
+
+205
+00:15:17,970 --> 00:15:21,990
+الزمن الملائم للبكتيريا ان تحدث فيه أمراض يعني
+
+206
+00:15:21,990 --> 00:15:25,870
+أكتر ناس بيصاروا بالأمراض هم الأطفال اللي فيها
+
+207
+00:15:25,870 --> 00:15:30,170
+السن و بعدها بشوية انها تابتنها قبل ما تبدأ هذه
+
+208
+00:15:30,170 --> 00:15:36,870
+المرحلة الخطرة بنعطي ال vaccine زي ما حكينا قبل
+
+209
+00:15:36,870 --> 00:15:39,590
+لحظات انها poorly immunogenic و it should be
+
+210
+00:15:39,590 --> 00:15:42,810
+coupled to بقولنا كده protein to enhance
+
+211
+00:15:42,810 --> 00:15:44,150
+immunogenicity
+
+212
+00:15:46,100 --> 00:15:51,120
+الان احنا بنعرف فاكسين اسمه DPT صح؟ DPT ثلاثي،
+
+213
+00:15:51,120 --> 00:15:54,680
+Diphtheria Pertussis وTetanus، فهي فاكسين الان
+
+214
+00:15:54,680 --> 00:16:01,240
+رباعي في الأسواق، بيشمل التلاتة بالاضافة إلى ال
+
+215
+00:16:01,240 --> 00:16:06,040
+Hemophilus influenza type B فصار رباعي، صار عشانها
+
+216
+00:16:06,040 --> 00:16:11,450
+فاكسين رباعيخلصنا .. خدوا بالكوا دقا .. خلصنا
+
+217
+00:16:11,450 --> 00:16:16,250
+المعلومات المتعلقة بتلت ميكروبيرات Spetacoccus
+
+218
+00:16:16,250 --> 00:16:20,410
+pneumonia, Muflis influenza و Nacinamide bites
+
+219
+00:16:20,410 --> 00:16:27,020
+اللي بتكلم فيه مقصود عن البكتيرا الرقم تلتبتعرفوا
+
+220
+00:16:27,020 --> 00:16:30,140
+السيرمونيلا التعيفى؟ السيرمونيلا التعيفى إلها
+
+221
+00:16:30,140 --> 00:16:33,520
+vaccine و لو رجعتوا للمحاضرة تبعتنا هنقولوا ال
+
+222
+00:16:33,520 --> 00:16:37,260
+prevention by vaccine طبعا الآن ال vaccine طبعا
+
+223
+00:16:37,260 --> 00:16:41,420
+السيرمونيلا التعيفى في منه صينفين واحد بيستهدف
+
+224
+00:16:41,420 --> 00:16:46,480
+الكابسول فنحطه مع مين؟ مع الكابسول، مع دولة واحد
+
+225
+00:16:46,480 --> 00:16:50,300
+لكل البكتيريا كل البكتيريا اللي هي killed bacteria
+
+226
+00:16:50,300 --> 00:16:55,620
+killed bacteria يعني السيرمونيلا ممكن نكون واحد أو
+
+227
+00:16:55,620 --> 00:16:56,180
+ندمجها
+
+228
+00:17:02,020 --> 00:17:07,740
+طبعا تسمونه طيفي vaccine is indicated يوصف ليه أو
+
+229
+00:17:07,740 --> 00:17:12,820
+يوصى بيه لمين to people who are traveling to areas
+
+230
+00:17:12,820 --> 00:17:17,620
+where typhoid fever is common يعني الحمض تيفؤيد
+
+231
+00:17:17,620 --> 00:17:24,240
+كان موجود أو منتشر بشكل كبير أو لأشخاص who are in
+
+232
+00:17:24,240 --> 00:17:28,420
+contact with typhoid fever patients or carriers
+
+233
+00:17:29,860 --> 00:17:36,620
+عشان احنا عارفين انه الانتقال في كالقول و الاحتكاك
+
+234
+00:17:36,620 --> 00:17:41,080
+المباشر مع المرضى قد يعني انت عارفين given aseptic
+
+235
+00:17:41,080 --> 00:17:47,520
+technique لو راح تخيلها عملية معقدة جداو مهما ببنى
+
+236
+00:17:47,520 --> 00:17:50,540
+من جهد .. مهما ببنى من جهد في الacidic technique
+
+237
+00:17:50,540 --> 00:17:55,300
+إلا يصير فيه breach خلل معين وبالتالي قد ينتقل
+
+238
+00:17:55,300 --> 00:17:59,960
+المرض فلما أنا أكون بتعامل مع مرضى و أنا at high
+
+239
+00:17:59,960 --> 00:18:05,590
+risk لما ممكن أاخد ايه؟ vaccine في هذه الحلقةالنوع
+
+240
+00:18:05,590 --> 00:18:07,710
+التالت من ال vaccine دو اللي عنه هو ال toxoid
+
+241
+00:18:07,710 --> 00:18:11,090
+vaccine و ال toxoid زي ما حكينا هي عبارة عن toxin
+
+242
+00:18:11,090 --> 00:18:15,010
+that has been modified كلمة modified قد تكون ال
+
+243
+00:18:15,010 --> 00:18:19,170
+modification chemical قد تكون physical قد تكون
+
+244
+00:18:19,170 --> 00:18:22,050
+genetic engineering يعني ممكن نعمل modification
+
+245
+00:18:22,050 --> 00:18:27,750
+بالهندسة الوراقية ناخد أول مثال Cornebacterium
+
+246
+00:18:27,750 --> 00:18:32,870
+diphtheria vaccine حد سمع منكوا أن فلان أصيب بال
+
+247
+00:18:32,870 --> 00:18:37,400
+diphtheriaمافيش، ليش مافيش؟ لأن الطلاب العربية
+
+248
+00:18:37,400 --> 00:18:41,320
+indicated يعني immunization against diphtheria is
+
+249
+00:18:41,320 --> 00:18:44,940
+indicated for every child، يعني حضراتكم لما كنتوا
+
+250
+00:18:44,940 --> 00:18:49,520
+child، كنتوا children، أخدتها للفاكسينكل العالم
+
+251
+00:18:49,520 --> 00:18:54,860
+اللي بيصلوا .. اللي بيفططوا الحضارة بياخد الـDPT
+
+252
+00:18:54,860 --> 00:18:59,100
+كنزه من الفكس، لذلك نسبة الدفتيريا تقريبا .. مش
+
+253
+00:18:59,100 --> 00:19:03,540
+تقريبا في غزة، إلها من سنوات صفر، إلها من سنوات
+
+254
+00:19:03,540 --> 00:19:10,560
+صفر، يعني الـToxoid لحاله كان كفيل بإنه يحمينا من
+
+255
+00:19:10,560 --> 00:19:13,240
+ال .. ال .. ال .. ال .. هذا .. هذا نقطة من فكرة
+
+256
+00:19:24,760 --> 00:19:28,480
+أنا بحكي ك تكسويد مش ك fragment من البكتيريا أخرى،
+
+257
+00:19:28,480 --> 00:19:30,840
+بس لأه .. لإن هو أصلا راح نكون نستخدمها ككاريا،
+
+258
+00:19:30,840 --> 00:19:34,840
+فهي كتيريا كبيرة، هو كبير، فبتاعي immunogenic جيد،
+
+259
+00:19:34,840 --> 00:19:39,700
+immunogenic جيد، أنا فهمت سؤالك لأه متاخرة، إنه ..
+
+260
+00:19:39,700 --> 00:19:43,680
+هو أنا ب .. ب .. بتكلم على ال fragment، إنه أنا
+
+261
+00:19:43,680 --> 00:19:48,020
+خدت تكسويد لحاله، ماخدتش معاه مراكبات أخرى كبسول
+
+262
+00:19:48,020 --> 00:19:50,340
+ولا خدت معاه أنتجلات تانية
+
+263
+00:19:54,090 --> 00:19:57,070
+كده المرة عرفنا انه بيعطل منين؟ for every child
+
+264
+00:19:57,070 --> 00:20:02,010
+على تلت جرعات اتنين اربع اشتهور من الامر و بعد سنة
+
+265
+00:20:02,010 --> 00:20:05,570
+من اخر جرعة يعني بيسموها booster dose اللي هي
+
+266
+00:20:05,570 --> 00:20:09,250
+الجرعة الداعمة او الجرعة التنشيطية اللي هي اسمها
+
+267
+00:20:09,250 --> 00:20:13,710
+باللغة العربية اما داعمة وهي الصحيحة الترجمة
+
+268
+00:20:13,710 --> 00:20:17,030
+الحرفية هي ال boost معناها دعم فbooster dose لكن
+
+269
+00:20:17,030 --> 00:20:21,190
+احنا برضه طبيا بيطلق على الجرعة التنشيطية اللي
+
+270
+00:20:21,190 --> 00:20:27,490
+بتذكر جهاز المناعرقم اتنان طبعا لحظه الأولاني اللي
+
+271
+00:20:27,490 --> 00:20:31,810
+هو في الجوجل الأولى it is formaldehyde treated ايش
+
+272
+00:20:31,810 --> 00:20:35,730
+يعني formaldehyde treated يعني اولج بمادة اللي هو
+
+273
+00:20:35,730 --> 00:20:39,990
+ال formaldehyde رقم اتنان Clostridium Tetani او
+
+274
+00:20:39,990 --> 00:20:43,150
+Clostridium Tetani vaccine contains tetanus toxoid
+
+275
+00:20:43,150 --> 00:20:46,970
+and is given to everyone زي نفسي ما هو الدول
+
+276
+00:20:46,970 --> 00:20:52,160
+الفلافيةearly in life وممكن بعد فترة وكل فترة يعني
+
+277
+00:20:52,160 --> 00:20:54,820
+هو غالبا بيعطي protection لخمس سنوات لو أعطينا
+
+278
+00:20:54,820 --> 00:21:00,540
+كمان جوع على خمس سنوات فبينشط اللي لازم الان
+
+279
+00:21:00,540 --> 00:21:04,340
+لاحظوا الأول والتاني تكسويت تكسويت وكانوا بالفعل
+
+280
+00:21:04,340 --> 00:21:08,100
+تتنسع اننا تقريبا شبه zero بياخدوا شبه zero تيجي
+
+281
+00:21:08,100 --> 00:21:10,560
+للقه تلاتة اللي هي ال borderline laparotosis
+
+282
+00:21:10,560 --> 00:21:10,940
+vaccine
+
+283
+00:21:13,620 --> 00:21:17,240
+but includes other proteins as well، خدوا بالكم،
+
+284
+00:21:17,240 --> 00:21:23,700
+اللي في غزة DBT، و ال DBT هي Deuterium Pertosis
+
+285
+00:21:23,700 --> 00:21:27,980
+Tetanus والتلاتة دوكسول فقط لالي، we are protected
+
+286
+00:21:27,980 --> 00:21:32,040
+من ال vaccine هذا، يعني حمينا من ال Deuterium و
+
+287
+00:21:32,040 --> 00:21:35,260
+حمينا من ال Tetanus، لكن we are not totally
+
+288
+00:21:35,260 --> 00:21:41,020
+protected من اللي هو ال ال ال ال Pertosisو أكبر دي
+
+289
+00:21:41,020 --> 00:21:43,920
+عيبالك ان كل اللي عالم اللي بيستخدم هذا ال vaccine
+
+290
+00:21:43,920 --> 00:21:47,380
+برضه مش protected against this disease وكان في
+
+291
+00:21:47,380 --> 00:21:51,620
+الكيان المحتر للغاصب كان قبل عدة سنوات ممكن
+
+292
+00:21:51,620 --> 00:21:55,840
+خمسمائة حالة سجلوه من ال pertosis وان في غزة في
+
+293
+00:21:55,840 --> 00:22:00,140
+symptoms typical pertosis لكن الطبان يبدو انهم مش
+
+294
+00:22:00,140 --> 00:22:03,920
+مقتنعين ان في انا ذات بقى ال pertosisفطبعا هو
+
+295
+00:22:03,920 --> 00:22:08,260
+الpertosis self-limiting على الرغم من خطورته
+
+296
+00:22:08,260 --> 00:22:12,780
+وصعوبته إلا إنه حتى لو ما أعطينا علار غالبا
+
+297
+00:22:12,780 --> 00:22:16,120
+بالنسبالي لو كنت فيه ضعيفة جدا، قادرة جدا استاذ
+
+298
+00:22:16,120 --> 00:22:19,900
+بالي متعرف بينا، شو هو الpertosis؟ هو السعالة
+
+299
+00:22:19,900 --> 00:22:22,840
+الديكي، السعالة ديكي، بعمل كل شي أنا بسموه السعالة
+
+300
+00:22:22,840 --> 00:22:28,790
+الديكي مانتش كذا؟ال .. ال disease بيتكون .. عشان
+
+301
+00:22:28,790 --> 00:22:32,310
+ذلك لازم نشرح أولاد مولودة من فهم أعمق يا بنات فهم
+
+302
+00:22:32,310 --> 00:22:36,590
+أعمق للمتباعة ال genesis أو لأي مسألة في الدنيا
+
+303
+00:22:36,590 --> 00:22:41,330
+بيقدر يتخذ قرار أفضل و أنا دايما بقول مثال بقول
+
+304
+00:22:41,330 --> 00:22:48,210
+أنه الشخص في المنزل قد يعني يتعرض لقرار يعتبره
+
+305
+00:22:48,210 --> 00:22:54,870
+ظالم من رب الأسرة من الوالد يعتبره ظالم و غير راضي
+
+306
+00:22:54,870 --> 00:22:59,160
+عن هذا القرارو انا بقول انه لأ يعني لو انت عرفت
+
+307
+00:22:59,160 --> 00:23:03,560
+البعلومات اللي عند رب الأسرة ربما اتخذت نفس القرار
+
+308
+00:23:03,560 --> 00:23:07,480
+و غالبا .. ده بت .. ممكن بتلاحظوا ال .. الامهات
+
+309
+00:23:07,480 --> 00:23:13,410
+بتعلم البنات و بيقولهم غلط و بيقولهم صح تمام؟طبعا
+
+310
+00:23:13,410 --> 00:23:17,230
+لو رجعنا بالتاريخ ما يلقوم كافية من أية بنت كانت
+
+311
+00:23:17,230 --> 00:23:20,170
+أما تقولها نفس الكلام اللي هي بتقولكواياه و هي ما
+
+312
+00:23:20,170 --> 00:23:22,970
+تسمعش الكلام و هي ماكانتش يسمع الكلام يعني سلسلة
+
+313
+00:23:22,970 --> 00:23:26,730
+من إيه لكن اللي أنا بوصلهياه أنه من يمتلك
+
+314
+00:23:26,730 --> 00:23:30,850
+المعلومات يمتلك المعلومات في شكل عام مش ضرور فيه
+
+315
+00:23:30,850 --> 00:23:32,970
+دراسة يعني و عندها كل المعلومات موجودة لكن بتاخد
+
+316
+00:23:32,970 --> 00:23:37,320
+قرار خاطرلكن هم اللي بيمتلك معلومات كثيرة غالبا
+
+317
+00:23:37,320 --> 00:23:42,880
+بياخد قراره مبني على قاعد أوسع من المعرفة فبالتالي
+
+318
+00:23:42,880 --> 00:23:48,680
+بيكون أفضل، ليش بنحك الكلام هذا؟ بنحك الكلام هذا
+
+319
+00:23:48,680 --> 00:23:52,880
+جاب ال .. طلبات اتكلمت عن ال bacteroidis
+
+320
+00:24:02,710 --> 00:24:07,310
+أنا عندي نية إن أنا أقوم بمحاضرة عامة عن ال normal
+
+321
+00:24:07,310 --> 00:24:11,030
+flora و عن الخير اللي بيحصل منها، لحظة تخيلوا
+
+322
+00:24:11,030 --> 00:24:15,830
+فصائل الدم is modified by normal flora، diabetes،
+
+323
+00:24:15,830 --> 00:24:19,630
+بلد السكر قد ينطقه، فيه دراسات ومؤشرات تقول إن
+
+324
+00:24:19,630 --> 00:24:23,650
+normal flora لها علاقة بهلوته، و نسبة انتشاره
+
+325
+00:24:24,440 --> 00:24:28,300
+المزاج تبعنا، إذا كان كويس أو مش كويس، إليه علاقة
+
+326
+00:24:28,300 --> 00:24:32,240
+بالنظر في الورق، نعم، فيعني إذا كانت تلعب بقى
+
+327
+00:24:32,240 --> 00:24:36,700
+بميرتنا و بتغير من ال mood تبعتنا، فبتجرب اللي ..
+
+328
+00:24:36,700 --> 00:24:40,340
+اللي .. اللي .. اللي بيخليك كده من الكلام إن
+
+329
+00:24:40,340 --> 00:24:46,650
+الإنسانغالبا بي .. بيحاول يقطف ثمار المعرفة مبكرا،
+
+330
+00:24:46,650 --> 00:24:50,550
+بمعنى انه بس يعرف معرفة جديدة، بيحاول يستغلها،
+
+331
+00:24:50,550 --> 00:24:54,470
+فمثلا، عرفنا ان البنتين هي اللي بتسيب الأمراض،
+
+332
+00:24:54,470 --> 00:25:01,020
+kill them all، اقتلوها99% بتستخدمها في 99.9% اللي
+
+333
+00:25:01,020 --> 00:25:05,580
+بتقول تمام؟ طهر إيدك، طهر إجليك، أهمال أكتر، خلاء
+
+334
+00:25:05,580 --> 00:25:09,020
+انتباطيك، وفي الآخر نكتشف إنه هذه الإجراءات
+
+335
+00:25:09,020 --> 00:25:13,260
+بتسببنا عمرها، مصبوط أو مش مصبوط؟ طبعاً الخير ..
+
+336
+00:25:13,260 --> 00:25:17,500
+الخير في Noble Flora ناتج عن التغيرات اللي .. اللي
+
+337
+00:25:17,500 --> 00:25:19,940
+.. اللي الإنسان في الغدا و التغيرات في الأدوية و
+
+338
+00:25:19,940 --> 00:25:22,540
+في العلاجات و في .. في .. في ..وفي طريق ال life
+
+339
+00:25:22,540 --> 00:25:25,140
+style تبعه وانا بتقولكوا مرة مهتمة انا بتقولكوا
+
+340
+00:25:25,140 --> 00:25:29,940
+انه تغيير النظام اللي يده إلى الأبقار أدى .. ده
+
+341
+00:25:29,940 --> 00:25:33,060
+الأبقار .. أدى كده انه يصير جزء من النظام اللي هو
+
+342
+00:25:33,060 --> 00:25:39,380
+تلاتة equalized أو 15787يبقى فعلا من العميق
+
+343
+00:25:39,380 --> 00:25:47,220
+بيعطينا إمكانية التدخل بدون ما نقضي ال .. ال .. ال
+
+344
+00:25:47,220 --> 00:25:49,860
+.. ال .. ال .. البيئة و نقضي ال .. ال .. ال .. ال
+
+345
+00:25:49,860 --> 00:25:53,220
+.. الbalance اللي على مدار القرون موجود
+
+346
+00:25:53,220 --> 00:25:58,120
+البورميلابيكوس كنا فاكرين إن الـtoxoid أو الـtoxin
+
+347
+00:25:58,120 --> 00:26:02,220
+هو ال major phytonic component of the bacteria
+
+348
+00:26:05,100 --> 00:26:08,800
+في الدفتيرك كرابنا صحيح في التتروس كرابنا صحيح لكن
+
+349
+00:26:08,800 --> 00:26:13,020
+في كرابنا مش صحيح لإنه لجهر البكتيريا اللي ها هيم
+
+350
+00:26:13,020 --> 00:26:17,920
+أجلوتينين بيتصق في السيليا اللي موجودة في التراكيا
+
+351
+00:26:17,920 --> 00:26:21,100
+وبتنقل خلال ما زمينا قلنا في الحركة السيليا
+
+352
+00:26:21,100 --> 00:26:25,920
+reaction اللي هو أساس طرد السبيوتم اللي بيخرج من
+
+353
+00:26:25,920 --> 00:26:30,520
+رسم الإنسان وبالتالي الفاكسين الجديد بيحتوي على
+
+354
+00:26:31,260 --> 00:26:35,820
+بيحتوي على فلامنتاس هم أجلوتنين و البرتكتين و
+
+355
+00:26:35,820 --> 00:26:39,460
+دلوقتي المركبين اللي هم علاقة بالبعض في نفس
+
+356
+00:26:39,460 --> 00:26:43,340
+الثرائح البكية فالان ال vaccine اللي موجود في
+
+357
+00:26:43,340 --> 00:26:50,960
+أمريكا بيحتوي على التكسور بقى كما هو زائد اللي هو
+
+358
+00:26:50,960 --> 00:26:54,660
+ال فلامنتاس هم أجلوتنين زائد اللي هو البرتكتين
+
+359
+00:26:54,660 --> 00:26:56,840
+اللي هم ال components of the cell wall
+
+360
+00:27:00,320 --> 00:27:04,300
+هنبدأ ان شاء الله مشوار السيمنارات للطالبات، كل
+
+361
+00:27:04,300 --> 00:27:08,600
+طالبة هتقدم خمس دقائق موضوع ان شاء الله يعني جديد
+
+362
+00:27:08,600 --> 00:27:12,120
+في ال microbiology ليس بضرورة يكونوا علاقة في ال
+
+363
+00:27:12,120 --> 00:27:15,260
+medical microbiology، لكن على الأقل بوسع مداركنا
+
+364
+00:27:15,260 --> 00:27:19,080
+ومفاهيمنا وهنبدأ ان شاء الله اليوم مع نور الصفة
+
+365
+00:27:19,080 --> 00:27:24,320
+دي، أول presentation اللي هتحاول تقدمها خلال خمس
+
+366
+00:27:24,320 --> 00:27:24,380
+دقائق
+
+367
+00:27:29,230 --> 00:27:32,850
+أول اسم البكتيريا موجودة في كل مكان، موجودة في
+
+368
+00:27:32,850 --> 00:27:36,150
+أجسامنا، في النبات، في الحيوانة، في كل مكان، منها
+
+369
+00:27:36,150 --> 00:27:39,450
+ما هو مفيد، منه ما هو ضاد اليوم راح أحكيلكم عن
+
+370
+00:27:39,450 --> 00:27:42,610
+إحدى فوائد البكتيريا إن البكتيريا بتنتج لنا
+
+371
+00:27:42,610 --> 00:27:46,150
+إلكترونات عن طريق هذه الإلكترونات بتستطيع إنولد
+
+372
+00:27:46,150 --> 00:27:50,050
+كهربا، فسموها هذه البكتيريا fuel microorganism
+
+373
+00:27:50,050 --> 00:27:56,470
+buttery bacteriaهذه البكتيريا من .. من أنواع هذه
+
+374
+00:27:56,470 --> 00:27:59,190
+البكتيريا نسأل على هذه البكتيريا اللى هى بكتيريا
+
+375
+00:27:59,190 --> 00:28:02,830
+ال Rodiferrix ferradinaceae هذه البكتيريا هى من
+
+376
+00:28:02,830 --> 00:28:06,030
+عائلة ال common denisius هذه من ال genius
+
+377
+00:28:06,030 --> 00:28:10,590
+Rodiferrix species ferradinaceae هذه البكتيريا أول
+
+378
+00:28:10,590 --> 00:28:13,290
+مرة عزلوها من رواسف Vanguela
+
+379
+00:28:17,760 --> 00:28:21,760
+هذه البكتيريا إلها خصائص تخلّيها مختلفة عن باقي
+
+380
+00:28:21,760 --> 00:28:25,660
+البكتيريات، هادي أن أثناء عملية تنفسها تستخدم
+
+381
+00:28:25,660 --> 00:28:29,280
+الحديد في التنفس تبعها، و بتتغذى زي أي نوع من
+
+382
+00:28:29,280 --> 00:28:35,500
+أنواع البكتيريا على الجليكوز، سيليلوز، استكاروزهذه
+
+383
+00:28:35,500 --> 00:28:39,520
+البكتيريا انه زى ما قولت قبلها انه بتنتج إلكتروناط
+
+384
+00:28:39,520 --> 00:28:43,740
+عن طريقة تستطيع انها تولد كهربا من خلالها هلقيت
+
+385
+00:28:43,740 --> 00:28:48,520
+كيف هي راح تولد الكهربا؟ قولت انه هي بتستخدم أثناء
+
+386
+00:28:48,520 --> 00:28:52,880
+عملية التنفس الحالية و بتستخدم كمان الجليكوز كمصدر
+
+387
+00:28:52,880 --> 00:28:56,180
+للغذاء لها، بتروح عن طريقهم، بتكسلهم فبتنتج
+
+388
+00:28:56,180 --> 00:29:00,310
+إلكتروناط، هذه الإلكتروناطبتعطينا كهربا وبتفضل انه
+
+389
+00:29:00,310 --> 00:29:03,930
+تعمل هذه العملية عن درجة حرارة من 4 درجة سيليزيا ل
+
+390
+00:29:03,930 --> 00:29:07,830
+30 درجة سيليزيا ولكن ال optimum لها 25 درجة
+
+391
+00:29:07,830 --> 00:29:12,890
+سيليزيا كيف انه نكشف عنها هى مبدأ العمل سكر جليكوز
+
+392
+00:29:12,890 --> 00:29:17,410
+مع ميه بوجود الحديد رح يعطينا CO2 مع الحديد نفس
+
+393
+00:29:17,410 --> 00:29:20,910
+الشغل مع ايونات الهيدروجين وحيكون الالكترونات هي
+
+394
+00:29:20,910 --> 00:29:25,390
+عبارة عن اولى عشان نفهم مبدأ العمل رح اورجيكم
+
+395
+00:29:25,390 --> 00:29:25,790
+فيديو
+
+396
+00:29:29,330 --> 00:29:32,610
+أول شغلة أن النبات راح يعمل عمليات بناء الطوائف
+
+397
+00:29:32,610 --> 00:29:49,490
+راح ينتج من خلاله سكر ال jericots ال
+
+398
+00:29:49,490 --> 00:29:52,030
+macro organism نعتبرها اللي هي ال Rodoflax في ال
+
+399
+00:29:52,030 --> 00:29:56,030
+ردينيز راح تتغذى عليها فرح تنتجلنا ال CO2 و رح
+
+400
+00:29:56,030 --> 00:29:57,530
+تنتجلنا إلكترونات
+
+401
+00:30:08,420 --> 00:30:11,520
+هلقيت رح نحطها تعتبر ان احنا حطيناها في جهاز
+
+402
+00:30:11,520 --> 00:30:14,120
+بيحتوي على انود و على كاتوب و على ال membrane
+
+403
+00:30:14,120 --> 00:30:17,040
+المادة اللي جرافيك ال membrane المادة اللي جرافيك
+
+404
+00:30:17,040 --> 00:30:20,340
+وظيفته ان يسهل عملية نقل الألوانات الهاروجين من
+
+405
+00:30:20,340 --> 00:30:24,060
+ناحية الانود لناحية الكاتوب هلقيت الإلكترونات بما
+
+406
+00:30:24,060 --> 00:30:28,240
+انها positive charge رح .. negative charge رح
+
+407
+00:30:28,240 --> 00:30:32,700
+تنتقل عن الانود لمصباح الكهربائي عشان نشوف إذا هي
+
+408
+00:30:32,700 --> 00:30:34,600
+هتولد من الكهربا ولا مش هتولد
+
+409
+00:30:54,390 --> 00:30:57,810
+بما أن الضواط يعني أش تحتوى على إلكترونات و إنها
+
+410
+00:30:57,810 --> 00:31:06,110
+فعلا بتولد كهربا راح ترجع طبعا على ال cathode و
+
+411
+00:31:06,110 --> 00:31:09,930
+راح تربط ما بين ال oxygen و ال hydrogen اللي انتقل
+
+412
+00:31:09,930 --> 00:31:13,910
+من الأنوال لل cathode و بتنتج لنا من خلاله ميه هذه
+
+413
+00:31:13,910 --> 00:31:15,770
+المبدأ العملية بشكل مختصر
+
+414
+00:31:23,740 --> 00:31:26,720
+وهي شكل العملية مرة تانية أنه قانون .. إجت
+
+415
+00:31:26,720 --> 00:31:29,860
+البكتيريا أنتجتنا إلكينات من القانون لنصبح
+
+416
+00:31:29,860 --> 00:31:34,380
+الكهربائي للكيثود فانتجتنا بعدها ميه شو المستقبل
+
+417
+00:31:34,380 --> 00:31:37,300
+هذه البكتيريا؟ هذه البكتيريا لو إجلنا بدنا نقارنها
+
+418
+00:31:37,300 --> 00:31:40,920
+بينها و بين كمية الكهربا اللي بتنتج في العالم طبعا
+
+419
+00:31:40,920 --> 00:31:44,140
+و بين الشركات طبعا الكمية بالنسبة لها كمية قليلة
+
+420
+00:31:44,140 --> 00:31:48,600
+بس المش .. المهم أنه هي البكتيريا هاي بتساعد على
+
+421
+00:31:48,600 --> 00:31:54,040
+إنتاج الكهربابنتخيل شوية ان هذه الكهربا وصلت عنا
+
+422
+00:31:54,040 --> 00:31:57,300
+لغزة .. اه هذه البكتيريا وصلت عنا لغزة و احنا
+
+423
+00:31:57,300 --> 00:32:00,420
+املنا ان الكهربا بدل ما تشير تجينا 8 ساعات تشير
+
+424
+00:32:00,420 --> 00:32:04,300
+على الأقل تجينا 10 ساعات، 12 ساعة فهذه البكتيريا
+
+425
+00:32:04,300 --> 00:32:09,040
+وصلت عنا وقدرنا نستغلها بشكل صحيح ووفرنا الأجهزة و
+
+426
+00:32:09,040 --> 00:32:12,360
+الإمكانيات من خلالها فانتجتنا الكهرباو في نفس
+
+427
+00:32:12,360 --> 00:32:17,580
+الوقت في بكتيريا تانية اسمها Chuanella Undecinens
+
+428
+00:32:17,580 --> 00:32:21,360
+هذه البكتيريا برضه موجودة في مياه المجاري هذه
+
+429
+00:32:21,360 --> 00:32:25,300
+البكتيريا لو ايضا اقدرنا نعزلها بتشتغل بنفس مبدأ
+
+430
+00:32:25,300 --> 00:32:30,560
+العمل و بتستطيع انه تنتجنا كهربا فلو وصلت لغزة مرة
+
+431
+00:32:30,560 --> 00:32:34,700
+تانية و استغلناها بشكل صحيح هترجع مرة تانية لغزة
+
+432
+00:32:34,700 --> 00:32:40,470
+تكون موضوع شكرابسم الله الرحمن الرحيم اليوم
+
+433
+00:32:40,470 --> 00:32:43,930
+موضوعنا هيتحدث عن نوع من أنواع البكتيريا بعمل على
+
+434
+00:32:43,930 --> 00:32:50,550
+تحويل فصائل الدم المختلفة من a او b او ab إلى
+
+435
+00:32:50,550 --> 00:32:53,890
+الفصيلة o باستعمال نوعين من أنواع البكتيريا ال
+
+436
+00:32:53,890 --> 00:32:57,990
+bacterium fragilis and النوع التاني هو عبارة عن
+
+437
+00:32:57,990 --> 00:33:01,210
+الفلافوبكتيريا meningospectum ويلها اسم اخر وهي
+
+438
+00:33:01,210 --> 00:33:03,730
+عبارة او الاسم بتاعنا مشهورة فيه هي
+
+439
+00:33:03,730 --> 00:33:06,710
+elizabethkingia meningospectum هذا هو النوع الاول
+
+440
+00:33:06,710 --> 00:33:12,330
+من البكتيرياbacteria it's pressure loss هى الأنواع
+
+441
+00:33:12,330 --> 00:33:15,810
+او الخصائص اللى تتمتع بيها هذا النوع من البكتيريا
+
+442
+00:33:15,810 --> 00:33:19,810
+طبعا obligate aerobic واخدنا في مصابق عن معنى
+
+443
+00:33:19,810 --> 00:33:22,470
+obligate aerobic و قعدين ال gram negative بصلاي
+
+444
+00:33:22,470 --> 00:33:25,610
+وهى الصورة موضحالنا تحت ال microscope بعد ما عملنا
+
+445
+00:33:25,610 --> 00:33:28,990
+لها stain و بتأكدنا انها negative بصلاي و هي عبارة
+
+446
+00:33:28,990 --> 00:33:31,810
+عن normal flora موجودة in intestinal attract in
+
+447
+00:33:31,810 --> 00:33:36,270
+human كولونز بتكون في حالتها الطبيعيةبتبقى flora
+
+448
+00:33:36,270 --> 00:33:39,410
+microflora أو normal flora لكن في حالة ال abnormal
+
+449
+00:33:39,410 --> 00:33:42,570
+conditions فورا بتتحول الى infections بتسبب ال
+
+450
+00:33:42,570 --> 00:33:46,130
+endogenous infections طبعا هي غير متحركة و ليست من
+
+451
+00:33:46,130 --> 00:33:48,230
+أنواع ال endosporous لأن ال endosporous هي كانت
+
+452
+00:33:48,230 --> 00:33:51,210
+لها نوعين مشهورين، فيهم هي عبارة عن الفاسيغاس
+
+453
+00:33:51,210 --> 00:33:54,990
+والبنستريديا طبعا هي أيضا من أنواع البكتيرا
+
+454
+00:33:54,990 --> 00:33:58,990
+المقاومة للبنسلين حيث تمت على إنتاج لل beta
+
+455
+00:33:58,990 --> 00:34:04,220
+-lactamaseأيضًا نستطيع عزلها عن طريق نوع من أنواع
+
+456
+00:34:04,220 --> 00:34:07,140
+ال agar هو عبارة عن ال bacterium spill esculin
+
+457
+00:34:07,140 --> 00:34:11,020
+agar يرمز بالرمز BBA هذا النوع من البكتيريا بتروح
+
+458
+00:34:11,020 --> 00:34:16,600
+بتحولي أو بتحللي ال esculin بتحوله ل dextrose and
+
+459
+00:34:16,600 --> 00:34:20,580
+esculetin فبتروح تتفاعل هذه المكونات لنتجات مع
+
+460
+00:34:20,580 --> 00:34:24,980
+الفرق iodines أو الفرق ions هذا الفرق موجود بشكل
+
+461
+00:34:24,980 --> 00:34:28,300
+طبيعي في داخل هذا النوع من ال mediaتمام بعد هيك
+
+462
+00:34:28,300 --> 00:34:31,520
+بيروح بيتحول للوسط حوالين ال coolness من dark
+
+463
+00:34:31,520 --> 00:34:34,680
+brown إلى black color و طبعا هذا النوع من
+
+464
+00:34:34,680 --> 00:34:37,880
+البكتيريا يعاد selective media إلى ال isolation
+
+465
+00:34:37,880 --> 00:34:41,600
+and identification of this microbe هلا ال
+
+466
+00:34:41,600 --> 00:34:44,220
+pathogens هي اللي هتكوّنها، حكينا هي موجودة في شكل
+
+467
+00:34:44,220 --> 00:34:47,480
+طبيعي ال normal flora intestinal tract في حالة ال
+
+468
+00:34:47,480 --> 00:34:50,320
+abnormal conditions بتعملي pathogensهذه ال
+
+469
+00:34:50,320 --> 00:34:52,900
+pathogens ممكن تتطور و تصل الى أنحاء عديدة في
+
+470
+00:34:52,900 --> 00:34:56,800
+الجسم ممكن تصل ل cns ال cns هو عبارة عن ال central
+
+471
+00:34:56,800 --> 00:35:00,040
+nervous system و ممكن تصل ل ال head لنيك ل ال
+
+472
+00:35:00,040 --> 00:35:04,950
+tissues soft tissues ل ال skinالتيترا عندى طبعا هى
+
+473
+00:35:04,950 --> 00:35:08,590
+قادرة او هى اساسا من احد مكوناتها ال capsule ال
+
+474
+00:35:08,590 --> 00:35:11,610
+capsule تبعها يعاد polysaccharides هذا بده يحميها
+
+475
+00:35:11,610 --> 00:35:15,550
+من الفاجوسيتوسس انها تكون بمهاجمتها و كذلك بتعمل
+
+476
+00:35:15,550 --> 00:35:18,430
+على إنتاج ال abscess formations اللي هي الخراج
+
+477
+00:35:18,430 --> 00:35:24,030
+اضافة ان ذلك بتسبب للبكتيريا في حالة انها وصلت على
+
+478
+00:35:24,030 --> 00:35:29,250
+الدم بشكل غير طبيعى و ايضا بتسبب لألم فى البطن هى
+
+479
+00:35:29,250 --> 00:35:35,320
+الصورة المضحكة عنها و بتسبب لبعض من الدياريالل
+
+480
+00:35:35,320 --> 00:35:37,880
+treatment، العلاج من هذا النوع من البكتيريا، طبعا
+
+481
+00:35:37,880 --> 00:35:40,980
+عندي ال antimicrobial متروناغازولز and
+
+482
+00:35:40,980 --> 00:35:45,480
+كوليمفينيكول، بدي أعمل inhibitor لل produce اللي
+
+483
+00:35:45,480 --> 00:35:48,000
+هي بتنتجها اللي هي ال beta-lactamase أو ال beta
+
+484
+00:35:48,000 --> 00:35:52,460
+-lactam، لو روحنا جمعنا بين ال beta-lactamase
+
+485
+00:35:52,460 --> 00:35:55,980
+inhibitions and ال beta-lactamase antibiotic، بهذا
+
+486
+00:35:55,980 --> 00:35:59,900
+الطريقة بقدر أنا أمنع من إنتاج هذا النوع عشان ما
+
+487
+00:35:59,900 --> 00:36:02,760
+تسببليش ال infections الموجودةاللأ ال medical
+
+488
+00:36:02,760 --> 00:36:05,480
+research حكيمها بداية أنها بتعمل على تحويل فصائل
+
+489
+00:36:05,480 --> 00:36:11,320
+الدم المختلفة من A أو B أو AB إلى فصيلة O فبالتالي
+
+490
+00:36:11,320 --> 00:36:13,800
+ال medical راح نستعملها في ال blood طبعا هي
+
+491
+00:36:13,800 --> 00:36:16,440
+بتستعمل في مجالات تانية زي ال cancer لكن اختصارا
+
+492
+00:36:16,440 --> 00:36:18,920
+للمقتر هنتحدث فقط عن ال blood ال
+
+493
+00:36:18,920 --> 00:36:22,700
+bacteriophagelosis بتعملي على إنتاج أنزيم معين
+
+494
+00:36:22,700 --> 00:36:26,620
+بتقدر فيه تحويل فصيلة الدم بالتحديد الفصيلة O إلى
+
+495
+00:36:26,620 --> 00:36:32,740
+الفصيلة A الفصيلة B إلى الفصيلة Oهذه الطريقة بتكون
+
+496
+00:36:32,740 --> 00:36:36,260
+عن طريق تكسير لل antigenats اللى موجودة على ال
+
+497
+00:36:36,260 --> 00:36:39,980
+surface لل RBCs الإنزيم اللى بتمل على إنتاجه هو
+
+498
+00:36:39,980 --> 00:36:43,200
+عبارة عن Alpha Galactosidase لإن أحد مكونات ال
+
+499
+00:36:43,200 --> 00:36:47,120
+antigen اللى موجود على ال RBCs اللى خاص بالفصيلة B
+
+500
+00:36:47,120 --> 00:36:52,260
+هو عبارة عن Galactose فانا لو جبتله نوع يعمل لي
+
+501
+00:36:52,260 --> 00:36:55,740
+Galactase اللى هو إنزعيم يكسر هذا نوع فطرقيا رح
+
+502
+00:36:55,740 --> 00:36:59,430
+تتحول إلى فصيلة O لإن فصيلة O خاليةتماما من أي
+
+503
+00:36:59,430 --> 00:37:02,410
+واحد من ال antigenats هذا بتحدث ليه؟ لإنه طبعا ال
+
+504
+00:37:02,410 --> 00:37:06,190
+goodbye A ب O blood group راح يتحدث بشكل عام بيحكي
+
+505
+00:37:06,190 --> 00:37:09,570
+لإنه الناس الفاصلة دمهم O عندهم antigenats على ال
+
+506
+00:37:09,570 --> 00:37:12,810
+RBCs من نوع A الفاصلة دمهم A عندهم antigenats من
+
+507
+00:37:12,810 --> 00:37:16,550
+نوع A والفاصلة دمهم B عندهم antigenats من نوع B
+
+508
+00:37:16,550 --> 00:37:20,250
+والفاصلة دمهم AB عندهم antigenats من نوع A و من
+
+509
+00:37:20,250 --> 00:37:24,500
+نوع B أما ال Oاللي هو الناعي فرقية نوربيه يعني لا
+
+510
+00:37:24,500 --> 00:37:28,640
+عندهم هذا ولا عندهم هذا النوع التكنيك بشكل مختصر و
+
+511
+00:37:28,640 --> 00:37:33,060
+بشكل سريع طبعا بتعملي على إنتاج كما حكينا نوع من
+
+512
+00:37:33,060 --> 00:37:35,840
+أنواع الأنظمات هو عبارة عن القلاد أنفا
+
+513
+00:37:35,840 --> 00:37:39,120
+-galactosidase بيروح بتعملي cut of the sugars in
+
+514
+00:37:39,120 --> 00:37:42,850
+surface of RBCs الخاص في دينالنوع التاني من
+
+515
+00:37:42,850 --> 00:37:46,510
+البكتيريا مابتصلناش فيه لإنه اقتصرا للوقت هو عبارة
+
+516
+00:37:46,510 --> 00:37:49,610
+عن ال Elizabeth King Young Hingospectrum أو الاسم
+
+517
+00:37:49,610 --> 00:37:51,950
+التاني المتعرف عليه أكتر هو عبارة عن
+
+518
+00:37:51,950 --> 00:37:54,610
+الفلافوبكتيريا من Hingospectrum النوع التاني من
+
+519
+00:37:54,610 --> 00:37:59,280
+البكتيريا بيحول فصلة الدم A إلى فصلة Oكيف؟ طبعا
+
+520
+00:37:59,280 --> 00:38:02,880
+نفس الطريقة بتعملى على إنتاج إنزيم خاص اسمه N
+
+521
+00:38:02,880 --> 00:38:06,480
+-acetylgalactamalanase
+
+522
+00:38:06,480 --> 00:38:10,320
+بتحول فاصلة الدم A إلى الفاصلة O بتخلص من ال
+
+523
+00:38:10,320 --> 00:38:14,240
+sugars الموجودة على ال surface لل RBCs و فورا
+
+524
+00:38:14,240 --> 00:38:18,280
+تتحول إلى الفاصلة Oهنا طبعا بشكل مختصر حكالة عن
+
+525
+00:38:18,280 --> 00:38:22,940
+البكتيريا desfragilosa بتعملى على إزالة أنتجين بيه
+
+526
+00:38:22,940 --> 00:38:25,000
+و بالنسبة للنوع التانى هو عبارة عن ال Elizabeth
+
+527
+00:38:25,000 --> 00:38:29,340
+King-Yamingospectrum بتتخلص من الفصيلة A طبعا هذا
+
+528
+00:38:29,340 --> 00:38:32,740
+النوع من البكتيريا برضه موجود in normal flora in
+
+529
+00:38:32,740 --> 00:38:35,180
+the intestinal tract و في حالة ال abnormal
+
+530
+00:38:35,180 --> 00:38:39,540
+conditions بتكون endogenous أو بتسببلى يعني أمراض
+
+531
+00:38:39,540 --> 00:38:43,210
+و infectionsقولنا كيف بدنا نستفيد او إيش السبب من
+
+532
+00:38:43,210 --> 00:38:47,330
+إنه اكتشفوا هذه الأنزيمات في تحويل فصائل لدم لإن
+
+533
+00:38:47,330 --> 00:38:50,750
+في عندي مثلا أنا مريض فصيلة دم بيه و الفصيلة
+
+534
+00:38:50,750 --> 00:38:53,190
+موجودة عندك في التلاجة مثلا أو المحفوظة عندك
+
+535
+00:38:53,190 --> 00:38:56,690
+فصيلتها ايه طب أنا كيف بدي أوصل ايه لبيه و هذا
+
+536
+00:38:56,690 --> 00:38:59,710
+الشخص في حالة مثلا مرض شديد أو لازم يعني أني
+
+537
+00:38:59,710 --> 00:39:03,570
+أجيبله فصيلة دم و إليه حيموت فكورا بستعمل أنزيمات
+
+538
+00:39:03,570 --> 00:39:06,770
+هذا النوع من البكتيريا و بحطها على هذا الدم طبعا
+
+539
+00:39:06,770 --> 00:39:10,310
+مش من شغلنا احنا من شغل تكاترةو فبتتحول تلقائيًا
+
+540
+00:39:10,310 --> 00:39:13,210
+فاصلة الدم إلى O متعارفة عليه طبعًا هي عبارة عن ال
+
+541
+00:39:13,210 --> 00:39:17,490
+universe blood cells يعني انها عالمية انها بتقدر
+
+542
+00:39:17,490 --> 00:39:22,290
+توزعلي الدم لجميع الفصائل التالية لإن O بتاعة B و
+
+543
+00:39:22,290 --> 00:39:26,790
+بتاعة A بتاعة AB طبعا فهيك تلقائيًا بتتحول فاصلة
+
+544
+00:39:26,790 --> 00:39:30,550
+الدم إلى O و بقدرزيتها أنا اسعف هذا المريض فبعطيه
+
+545
+00:39:30,550 --> 00:39:34,850
+من هذا الدم فبالتالي بتكون safety of clinical
+
+546
+00:39:34,850 --> 00:39:37,690
+transfusion شكرا لكم اسمي اسمي
+
+547
+00:39:41,820 --> 00:39:45,020
+طبعا اليوم هنحكي عن بكتيريا مهمة و تم اكتشافها
+
+548
+00:39:45,020 --> 00:39:50,280
+حديثا اسمها ال humans bacteria اول
+
+549
+00:39:50,280 --> 00:39:52,960
+شغل قبل ما نتعرف عن خصائص هذه البكتيريا اننا نعرف
+
+550
+00:39:52,960 --> 00:39:55,660
+ان هذه البكتيريا في عندها ميكانيزم خاص بتقوم فيه
+
+551
+00:39:55,660 --> 00:39:59,100
+اللي هو ال bioluminescence اول اشي هذه البكتيريا
+
+552
+00:39:59,100 --> 00:40:05,080
+هذه الخاصية بتقوم ببعث ضوء بتعمل emission للضوء
+
+553
+00:40:05,080 --> 00:40:08,540
+طبعا تعمل emission للضوء بوسط انزيم اسمه ال
+
+554
+00:40:08,540 --> 00:40:13,900
+luciferaseبحكي إن هذه البكتيريا بتكون موجودة
+
+555
+00:40:13,900 --> 00:40:17,820
+بأشياء كتيرة زي الفنجاي و طبعا المسكرون بتكون
+
+556
+00:40:17,820 --> 00:40:21,560
+موجودة بالبكتيريا و بتكون موجودة على ال gut tract
+
+557
+00:40:21,560 --> 00:40:23,920
+و ال skin surface لبعض ال marine اللي موجودة في
+
+558
+00:40:23,920 --> 00:40:29,840
+المحيطات و البحار هذه صور بتوضح لنا كيف البكتيريا
+
+559
+00:40:29,840 --> 00:40:30,700
+بتعطي ضوء
+
+560
+00:40:34,050 --> 00:40:37,290
+طبعا هذه البكتيريا منشورة بشكل واسع زي ما حكينا في
+
+561
+00:40:37,290 --> 00:40:40,190
+ال sea water و في ال sheens المحيطات و بال
+
+562
+00:40:40,190 --> 00:40:44,370
+freshwater كمان بيقول إن هذه البكتيريا قادرة على
+
+563
+00:40:44,370 --> 00:40:48,390
+إنها تعمل علاقة تكافلية مع بعض الكائنات البحرية زي
+
+564
+00:40:48,390 --> 00:40:52,390
+ال fish و ال scutes اللي هي السرطانات ال crepes و
+
+565
+00:40:52,390 --> 00:40:58,470
+الحبار و ال dolphins بعض خصائص البكتيريا إن هي
+
+566
+00:40:58,470 --> 00:41:02,610
+root sap و كمان gram negative و كمان هي البكتيريا
+
+567
+00:41:02,610 --> 00:41:09,000
+ديموتايو طبعا نوع المتايل flattened motion و طبعا
+
+568
+00:41:09,000 --> 00:41:19,760
+هي facultative in aerobics هذا
+
+569
+00:41:19,760 --> 00:41:22,020
+ال mechanism اللي بتقوم فيه البكتيريا فيه نسط
+
+570
+00:41:22,020 --> 00:41:26,940
+بسترات اسمها ال luciferin بوجود الأكسوجين بانزيم
+
+571
+00:41:26,940 --> 00:41:34,180
+اسمه ال luciferase بيعطينا ال lightهذه الميكانيزم
+
+572
+00:41:34,180 --> 00:41:37,340
+تبعتها طبعا بيحكي لإن كل بكتيريا .. هذه البكتيريا
+
+573
+00:41:37,340 --> 00:41:41,620
+عندها قدرة على إنتاج ال .. ال auto-inducer كل
+
+574
+00:41:41,620 --> 00:41:44,560
+بكتيريا بتعطيني auto-inducer طبعا ال auto-inducer
+
+575
+00:41:44,560 --> 00:41:48,940
+هذا احنا لما أخدنا خاصية ال biofilm في البكتيريا
+
+576
+00:41:48,940 --> 00:41:52,660
+عندها القدرة على عمل keram sensing فكل بكتيريا
+
+577
+00:41:52,660 --> 00:41:55,760
+بتعطيني auto-inducer واحد واللي بنظم هذه العملية
+
+578
+00:41:55,760 --> 00:42:01,980
+في عندنا gene اسمه Lux operin geneطبعا بيحكيلي ال
+
+579
+00:42:01,980 --> 00:42:05,820
+substrate بتاعة البكتيريا هذه في عندنا اللي هي
+
+580
+00:42:05,820 --> 00:42:10,780
+flavin mononucleotide مع ال O2 و بوجود ال
+
+581
+00:42:10,780 --> 00:42:15,520
+longitude من ال fatty aldehyde طبعا بالنهاية
+
+582
+00:42:15,520 --> 00:42:18,860
+بيعطيني طاقة و بيعطيني ضوء و طبعا الضوء اللي
+
+583
+00:42:18,860 --> 00:42:22,680
+بتعطيني هذه البكتيريا اللي هو blue green و ممكن
+
+584
+00:42:22,680 --> 00:42:29,620
+نشوفه على طول موج 490 nanometerهذا الكلام حكيناه
+
+585
+00:42:29,620 --> 00:42:34,020
+طوال طبعا الأصناف تبعت هذه البكتيريا تلت أصناف تم
+
+586
+00:42:34,020 --> 00:42:37,260
+اكتشافها اللي هي ال vibro و ال photo photo
+
+587
+00:42:37,260 --> 00:42:42,340
+bacterium و ال photo hydrous طبعا ال vibro في منها
+
+588
+00:42:42,340 --> 00:42:45,760
+نوعين ال fisher و ال .. و ال hearty
+
+589
+00:42:48,270 --> 00:42:51,050
+الميديات اللى بنستخدمها عشان ننمي هذه البكتيريا
+
+590
+00:42:51,050 --> 00:42:53,310
+الميديا مرت علينا الثيوسالفيتسايتريت
+
+591
+00:42:53,310 --> 00:42:57,550
+بايلسولتساكاروز ال TCPC-Agar والميديا التانية ال
+
+592
+00:42:57,550 --> 00:43:00,930
+modified sea water complete وطبعا بتاعتها لون أخضر
+
+593
+00:43:00,930 --> 00:43:06,270
+و أزرق على الميديا هاي صورة للميديا بنمي عليها
+
+594
+00:43:06,270 --> 00:43:10,510
+البكتيريا و هاي الألوان زى ما بتشايفين مش واضح
+
+595
+00:43:10,510 --> 00:43:13,290
+كتير أزرق و أخضر بيكون اللون
+
+596
+00:43:15,630 --> 00:43:18,530
+بعض التطبيقات لهذه البكتيريا بيقول إنهم استخدموها
+
+597
+00:43:18,530 --> 00:43:22,150
+في مجال ال food microbiology كمان استخدموها ك
+
+598
+00:43:22,150 --> 00:43:25,230
+biotechnology و استخدموها في ال drug diagnosis و
+
+599
+00:43:25,230 --> 00:43:29,050
+كمان هذه البكتيريا بيقول إنها بتعمل تقليل على ال
+
+600
+00:43:29,050 --> 00:43:34,390
+toxicity للwater و كمان تم اكتشافها في .. طبعا هذه
+
+601
+00:43:34,390 --> 00:43:38,090
+البكتيريا بتكشف عن موجود ال fossil fuels اللي هو
+
+602
+00:43:38,090 --> 00:43:41,670
+الوقود الوحفوري و بتستخدم في ال cold war اللي هي
+
+603
+00:43:41,670 --> 00:43:45,920
+الحرب الباردةطبعا هذه من مساويها و كمان بيقولي في
+
+604
+00:43:45,920 --> 00:43:48,720
+دراسة حديثة بتحكي أنهم استخدموا هذه البكتيريا في
+
+605
+00:43:48,720 --> 00:43:52,340
+إنارة الطرق و الشوارع و يعني هذا تطبيق جيد لو إحنا
+
+606
+00:43:52,340 --> 00:43:56,040
+استخدمناه و في دراسات كتير تمت على هذه البكتيريا و
+
+607
+00:43:56,040 --> 00:43:59,480
+ان شاء الله بيتم هنا دراسة بغزة و بيلقوا في البحر
+
+608
+00:43:59,480 --> 00:44:02,620
+تبعنا و بيستخلصوا البكتيريا و بنقدر إنه نضيق
+
+609
+00:44:02,620 --> 00:44:05,200
+الشوارع بدون .. وش كده؟
+
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/BMp05amsL4I_postprocess.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/BMp05amsL4I_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..32548a5dfefcd3994fce2d4cd1199ff7685f1a78
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/BMp05amsL4I_postprocess.srt
@@ -0,0 +1,2508 @@
+1
+00:00:20,790 --> 00:00:24,490
+بسم الله الرحمن الرحيم، الموضوع الجديد، بأنوان
+
+2
+00:00:24,490 --> 00:00:28,610
+Antimicrobial Drugs، هنركز فيه بشكل كبير على ال
+
+3
+00:00:28,610 --> 00:00:33,230
+mechanism of action لل drugs أو ال antibiotics إذا
+
+4
+00:00:33,230 --> 00:00:35,810
+بتلاحظوا، احنا جادين بنتكلم، بنقول Antimicrobial و
+
+5
+00:00:35,810 --> 00:00:38,950
+بنقول Antibiotics في نفس الوقت، و أكيد إنهم اتنين
+
+6
+00:00:38,950 --> 00:00:44,530
+واحد، لكن واضح إن احنا بنتكلم عن مصطلحين مختلفين
+
+7
+00:00:44,530 --> 00:00:45,770
+تماما، وبالتالي لنحاول
+
+8
+00:00:51,160 --> 00:00:54,100
+أنا مشترك في جزء كبير من التعريف، الجزء الأول من
+
+9
+00:00:54,100 --> 00:00:57,880
+التعريف ان they kill or inhibit microorganisms،
+
+10
+00:00:57,880 --> 00:01:02,060
+هذا متفق عليه، they kill or inhibit، و in small
+
+11
+00:01:02,060 --> 00:01:06,880
+concentration، يعني بتراجيز منخفضة جدا، لكن الفرق
+
+12
+00:01:06,880 --> 00:01:12,080
+بينهم ان انت بيتك في شرط لازم يكون naturalغالبا
+
+13
+00:01:12,080 --> 00:01:16,220
+كلمة natural source تعني انه .. انه .. انه مصطنبط
+
+14
+00:01:16,220 --> 00:01:19,220
+من بكتيريا او فطريات يعني محفوظ من بكتيريا او
+
+15
+00:01:19,220 --> 00:01:23,960
+فطريات بنما ال antimicrobial لم يشترط هذا الشرط
+
+16
+00:01:23,960 --> 00:01:28,520
+وبالتالي مين اعام ال antimicrobial اعام من ال
+
+17
+00:01:28,520 --> 00:01:32,920
+antibiotic فهيشبوب بقدر يقول بامان كل antibiotic
+
+18
+00:01:32,920 --> 00:01:37,240
+هو antimicrobial ولكن مضبط وليس كل antimicrobial
+
+19
+00:01:37,240 --> 00:01:42,540
+هو antibioticطبعا احنا كده مرة لا نقلل من اهمية
+
+20
+00:01:42,540 --> 00:01:45,580
+ذاكرة او هذا الاهمية التانية اللي هو استخداماتهم
+
+21
+00:01:45,580 --> 00:01:50,840
+ففي مثلا مراكزات سلفة مشهورة ومعروفة انها
+
+22
+00:01:50,840 --> 00:01:53,220
+synthetic مخلقة و هي تات ناتشران و هي
+
+23
+00:01:53,220 --> 00:01:57,720
+antimicrobial و هي safe و تستخدم استخدامها ردًا
+
+24
+00:01:57,720 --> 00:02:02,700
+ويلها يعني توظيف جيد في ال treatment المصطلح الأول
+
+25
+00:02:02,700 --> 00:02:06,760
+اللي على السطورة هو ال chemotherapyالكيموثيرابي هو
+
+26
+00:02:06,760 --> 00:02:11,720
+اي .. اي توظيف ل chemical drugs في ال treatment
+
+27
+00:02:11,720 --> 00:02:13,900
+طبعا هاجوله وقتها هوواش treatment ما هو chemical
+
+28
+00:02:13,900 --> 00:02:18,800
+drugs لأ فيه surgery صح؟ جراحة .. فيه natural
+
+29
+00:02:18,800 --> 00:02:23,580
+remedies، نباتات، فيه مساج، فيه تبليك .. لأ لكن
+
+30
+00:02:23,580 --> 00:02:28,440
+إذا موظفنا ال chemicals أو chemical drugs فيه،
+
+31
+00:02:28,440 --> 00:02:31,280
+احنا الشائعين عنها للأسف إنه يقولوا كيموثيرابي ليه
+
+32
+00:02:31,280 --> 00:02:36,180
+علاش كماوي، يعني كاسيلكن الواقع الأول هو توظيف ال
+
+33
+00:02:36,180 --> 00:02:40,060
+chemicals في ال treatments المصطلح الأخير هو
+
+34
+00:02:40,060 --> 00:02:43,820
+الأكثر أهمية على الإطلاق selective toxicity
+
+35
+00:02:43,820 --> 00:02:48,480
+بتعطيكم أسلامها قبل ما مقدمة قبل أسبوعين و أتلافة
+
+36
+00:02:48,480 --> 00:02:53,280
+نشرنا paper مع قسم الكيميا paper لنبحث هم صنعوا
+
+37
+00:02:53,280 --> 00:02:55,760
+بعض المركبات ال organic
+
+38
+00:02:59,870 --> 00:03:03,450
+هذه المراكبات انهم ينشروا هذه المراكبات انهم صنعوا
+
+39
+00:03:03,450 --> 00:03:07,430
+نوع جديد من المراكبات فانصحتهم و قلتلهم انه لأ
+
+40
+00:03:07,430 --> 00:03:12,290
+وقفه اللى بتصنعه ماتنشروش خلاص خدنا نعمله testing
+
+41
+00:03:12,290 --> 00:03:14,890
+نشوف هل هو antimicrobial activity و لا مش
+
+42
+00:03:14,890 --> 00:03:17,290
+antimicrobial activity و بالفعل لو ياله هو
+
+43
+00:03:17,290 --> 00:03:21,230
+antimicrobial activity الان نشرتنا ال paper اللى
+
+44
+00:03:21,230 --> 00:03:25,070
+هو البحث في مجلة عالمية محترمة بويسة و قبل البحث
+
+45
+00:03:25,070 --> 00:03:29,720
+انتشرتهل يمكن أستخدم مراكبتها للآن في العلاج
+
+46
+00:03:29,720 --> 00:03:33,480
+مباشرة؟ دولة على أنظر إلى ما يسمى ال selective
+
+47
+00:03:33,480 --> 00:03:37,300
+toxicity طبعا يمكن تكون زميسا من البكتيريا زميسا
+
+48
+00:03:37,300 --> 00:03:41,740
+وكلمة selective toxicity تعني it should be toxic
+
+49
+00:03:41,740 --> 00:03:44,500
+to the bacteria of the microorganism but not to
+
+50
+00:03:44,500 --> 00:03:49,500
+the host طبعا هذا ideally أنتوا بتسمعوا أنه معبر
+
+51
+00:03:49,500 --> 00:03:53,940
+ال antibiotics إلها بعض ال side effectsيعني
+
+52
+00:03:53,940 --> 00:03:57,820
+بنسالين ممكن تكون عندك hyper sensitivity بعض ال
+
+53
+00:03:57,820 --> 00:04:00,340
+antibiotic ممكن تعمل رارق بعض ال antibiotic ممكن
+
+54
+00:04:00,340 --> 00:04:07,940
+تعمل liver toxicity liver toxicity فالمصطلح هنا هو
+
+55
+00:04:07,940 --> 00:04:12,560
+على المستوى الخلوي أن يكون تأثيره على البكتيريا
+
+56
+00:04:12,560 --> 00:04:19,420
+وليس على خلالها العادى الطبق اللي قدامكوا هو الطبق
+
+57
+00:04:19,420 --> 00:04:24,790
+الشهير لالكزي .. لAlexander Flemingاللي كان مهمل
+
+58
+00:04:24,790 --> 00:04:28,850
+راح إجازة و سافر أطباق على الطاولة المخوضة هنا
+
+59
+00:04:28,850 --> 00:04:32,090
+إجرائية لازم نعقمهم قبل ما نروح على الإجازة يعني
+
+60
+00:04:32,090 --> 00:04:35,290
+يوم الأربع إذا عندنا الخميس و الجمعة إجازة لازم
+
+61
+00:04:35,290 --> 00:04:37,790
+نعقم كل أطباق ما نخليها موجودة في المطبق فدرك
+
+62
+00:04:37,790 --> 00:04:42,070
+الأطباق و راح إجازة و رجع لفت مقرره قبل ما يكتب
+
+63
+00:04:42,070 --> 00:04:46,730
+الأطباق أنه كان عنده طبق مليء بمستعمرات اللي
+
+64
+00:04:46,730 --> 00:04:49,630
+شايفينها هذه الـstaphylococcus aureus
+
+65
+00:04:52,850 --> 00:04:57,310
+ولا من فطر ولا من Penicillium وان المنطقة المحيطة
+
+66
+00:04:57,310 --> 00:05:00,690
+بالفطر بلغة المستعمرات تختفي تذوب ده فتلاحظ
+
+67
+00:05:00,690 --> 00:05:04,650
+المقارنة هنا وهنا يعني صغير جدا المستعمرات بواق
+
+68
+00:05:04,650 --> 00:05:09,010
+المستعمرات ومتأكد ان كان فيها مستعمرات كبيرة فكان
+
+69
+00:05:09,010 --> 00:05:12,050
+لابد ورا سنتاش منطقي لابد ان يكون هناك شيء في
+
+70
+00:05:12,050 --> 00:05:13,110
+الفطر قتله
+
+71
+00:05:17,420 --> 00:05:20,640
+طلعوا سنة الف وتسعمائة و كده؟ تمانية و عشرين،
+
+72
+00:05:20,640 --> 00:05:24,200
+اتطلعوا اول توظيف للبنسالين و ابتش كان؟ الف
+
+73
+00:05:24,200 --> 00:05:28,440
+وتسعمائة و أربعين، ده لما انا ابدا انتقال مركبات و
+
+74
+00:05:28,440 --> 00:05:31,400
+ادخلها في الخدمة العلاجية بيطلع بقطر، حتى في زمننا
+
+75
+00:05:31,400 --> 00:05:35,460
+هذا، زمن السرعة، بدنا من خمس الى خمساشر سنة حتى
+
+76
+00:05:35,460 --> 00:05:38,860
+نتكلم عن مركب ان ندخل في الخدمة العلاجية، لإن بمر
+
+77
+00:05:38,860 --> 00:05:45,350
+بمراحل testingطبعا هذا السيطرة على جشع الشركات
+
+78
+00:05:45,350 --> 00:05:49,810
+الأدوية اللي مقدت تزور في بعض الدراسات ففي مؤسسات
+
+79
+00:05:49,810 --> 00:05:52,850
+الآن زي ال FDA full of drugs administration تعمل
+
+80
+00:05:52,850 --> 00:05:55,730
+testing هي service لها لهادي الشركات تضمن إن هو
+
+81
+00:05:55,730 --> 00:06:00,010
+المركز بالفعل safe وحتى مع الضمان هذا للأسف كمان
+
+82
+00:06:00,010 --> 00:06:03,110
+لجينا بعض ال drugs اللي نزلت في السوق و بعدها تم
+
+83
+00:06:03,110 --> 00:06:06,310
+ايه ساعد على إنه بالفعل لجوا إليها side effect
+
+84
+00:06:06,310 --> 00:06:13,780
+مكان مرضي في 1940كل من هارد فلوري و إيرنست شين عمل
+
+85
+00:06:13,780 --> 00:06:17,320
+أول دراسات سيريرية و بعدها دخل الخدمة منذ اكتشاف
+
+86
+00:06:17,320 --> 00:06:21,240
+ال penicillin و تنظيفه بدأت الاكتشافات تبع ال
+
+87
+00:06:21,240 --> 00:06:24,900
+antibiotics تتوارى صار عننا يعني تخمة بال
+
+88
+00:06:24,900 --> 00:06:28,380
+antibiotics كتير عن ال antibiotics لدرجة اشعارنا
+
+89
+00:06:28,380 --> 00:06:33,220
+بالغرور انه علم موجود معانا لكل مايكروفات الدنيا
+
+90
+00:06:33,220 --> 00:06:33,940
+antibiotics
+
+91
+00:06:40,000 --> 00:06:43,440
+بتقول هذا باعطينا قائمة باسماء البكتيريا والفطريات
+
+92
+00:06:43,440 --> 00:06:47,820
+وال artenomycetes اللي بينتج منها أشهر أنواع ال
+
+93
+00:06:47,820 --> 00:06:51,900
+antibiotics مثلا ال bacillus satilis بينتج منها
+
+94
+00:06:51,900 --> 00:06:55,740
+البستراسين وال bacillus polymyxa بينتج منها
+
+95
+00:06:55,740 --> 00:06:59,260
+polymyxin B وهدول التنين Antibiotic بستراسين غير
+
+96
+00:06:59,260 --> 00:07:03,720
+مر معانا لما درسنا ال group A streptococci وميزنا
+
+97
+00:07:03,720 --> 00:07:06,420
+بينها بال Beta Hemolytic عملنا فحص البستراسين
+
+98
+00:07:13,370 --> 00:07:17,030
+بيوجين الكوكاية هو في البيوجين الكوكساية طيب
+
+99
+00:07:17,030 --> 00:07:21,790
+الأكتينو مايسيتس اللي قلتلكم مرة و لما كانت دي
+
+100
+00:07:21,790 --> 00:07:28,010
+نتيجتي أول مشيتها الأكتينو مايسيتس بالفعل they are
+
+101
+00:07:28,010 --> 00:07:30,110
+wonderful microbes يعني ميكروبات من الميكروبات
+
+102
+00:07:30,110 --> 00:07:36,140
+الرائعة جدا زمان أحكيلكم قصة زمانالعجايز ..
+
+103
+00:07:36,140 --> 00:07:38,080
+العجايز اللي هم كانوا صغار و بيولدوا يعني فيهم
+
+104
+00:07:38,080 --> 00:07:44,420
+بالأيام، لما ابنها كان يصير عنده أصماط، التهاب،
+
+105
+00:07:44,420 --> 00:07:46,740
+لما كان عنده ال border الترك زي اللي .. اللي
+
+106
+00:07:46,740 --> 00:07:51,460
+بيستخدموه اليوم، كانوا يجيبوا طينة حمرة جافة و
+
+107
+00:07:51,460 --> 00:07:53,380
+يطحنوها و ينخلوها عبر الشارع
+
+108
+00:08:04,750 --> 00:08:09,270
+فهر يعني لما يكون تراب جاف وغير مخلوق بمواد مؤذية
+
+109
+00:08:09,270 --> 00:08:16,990
+من مخلفات أدمية وغيرها وحيانية فهر طبعا الجزئين
+
+110
+00:08:19,250 --> 00:08:22,870
+كان تم لأن جزء من الطينة كان فيكون فيه antibiotics
+
+111
+00:08:22,870 --> 00:08:27,210
+لوجود ال actinomycetes في هذه البيئة فال
+
+112
+00:08:27,210 --> 00:08:30,730
+actinomycetes و يبتنمو بتنتج antibiotics و لإن
+
+113
+00:08:30,730 --> 00:08:35,230
+الغبار صار particles ضعيفة صغيرة جدا فمنعت من
+
+114
+00:08:35,230 --> 00:08:38,850
+اهتكاك الجلب للجد فقلبت من ايه؟ من التهيير جبتالي
+
+115
+00:08:38,850 --> 00:08:43,250
+ممكن كان سهر الشفاء إذا ال antibioticsقديما
+
+116
+00:08:43,250 --> 00:08:48,830
+معروفة، لكن المصريين علجوا .. القدراء علجوا مرضاهم
+
+117
+00:08:48,830 --> 00:08:52,350
+بالخبز المعفن، تمام؟ كانوا ياخدوا من الخبز .. و
+
+118
+00:08:52,350 --> 00:08:55,530
+الخبز المعفن ايش هو؟ بنسيمين، تمام؟ بنسيمين،
+
+119
+00:08:55,530 --> 00:09:00,910
+وبالتالي كان البشر قدرقوا في بعض الأحيان بفطرتهم،
+
+120
+00:09:00,910 --> 00:09:04,890
+بملاحظاتهم، لذا كانوا بيقولوا ان ال science is a
+
+121
+00:09:04,890 --> 00:09:06,210
+field of observation
+
+122
+00:09:09,680 --> 00:09:12,900
+الدربيشن هي كتير مهمة وفرق بين الناس .. انت
+
+123
+00:09:12,900 --> 00:09:20,280
+بتلاحظوا شغل ان معظم المكتشفين والولماء يلهم نظرة
+
+124
+00:09:20,280 --> 00:09:26,780
+مختلفة عن الآخرين في ال .. في الظواهر، الظواهر كل
+
+125
+00:09:26,780 --> 00:09:29,360
+بيشاهدها وكل بيشوفها لأن بيجي عالم
+
+126
+00:09:36,020 --> 00:09:41,140
+الحق المتمرس غالبا بيسأل الأسئلة اللى بتأدي إلى
+
+127
+00:09:41,140 --> 00:09:46,120
+نتائج من ضمن هذه الاكتشافات نرجع للأكتينوميسات
+
+128
+00:09:46,120 --> 00:09:50,000
+الرائع اللى قول عنها رائع streptomyosis nodosis
+
+129
+00:09:50,000 --> 00:09:53,360
+streptomyosis venezuela streptomyosis professions
+
+130
+00:09:56,160 --> 00:09:59,540
+نتعود عليهم Steratomyces nodosis هدولة مش ممرضات
+
+131
+00:09:59,540 --> 00:10:03,260
+هدولة منتجات هدولة يعني بكتيريا صديقة بكتيريا
+
+132
+00:10:03,260 --> 00:10:07,900
+صديقة Steratomyces Venezuela تنتج كلرمفينيكول خدوا
+
+133
+00:10:07,900 --> 00:10:11,340
+بالكم من كل القائمة رقم تلاتة اللي هو ال
+
+134
+00:10:11,340 --> 00:10:17,400
+Amphotericin B شايفينه؟ ورقم اتنين من تحت اللي هو
+
+135
+00:10:17,400 --> 00:10:22,040
+Glycepholvainأمفوترسين بي و graceful بي مانوا
+
+136
+00:10:22,040 --> 00:10:27,680
+بدولة هدولة التانين antifungal يعني بيقتلوا الفطر
+
+137
+00:10:27,680 --> 00:10:36,380
+يعني والباقي antibacterials يعني تقتل البركيلة
+
+138
+00:10:36,380 --> 00:10:40,080
+راحظوا إيش سمت ال .. ال .. ال .. ال .. ال anti تبع
+
+139
+00:10:40,080 --> 00:10:43,520
+الفطر ياسا منها antifungal تغيب ال antibiotic
+
+140
+00:10:43,520 --> 00:10:44,580
+مقصورة
+
+141
+00:10:52,500 --> 00:10:55,600
+Kills microorganisms صح؟ لكن هي واقع الأمر
+
+142
+00:10:55,600 --> 00:11:00,620
+الاستخدام الحقيقي لها غالب من ضد البكتيريا لأنه لو
+
+143
+00:11:00,620 --> 00:11:05,140
+قلنا الفانجل هو microorganism صح؟ لكن لما بنطلق
+
+144
+00:11:05,140 --> 00:11:09,420
+مركب ضدهش بنقول انتي فانجل لبروتزوفا بنقول انتي
+
+145
+00:11:09,420 --> 00:11:13,520
+بروتزوفا لدراج طبعا؟ اقولكوا حتى لميكوبكتيريا اللي
+
+146
+00:11:13,520 --> 00:11:17,320
+هي بكتيريا لما بنعطيها انتي بنقول انتي ميكوبكتيريا
+
+147
+00:11:17,320 --> 00:11:20,240
+علشان نميزها فغالب ال antibiotics هي ال common
+
+148
+00:11:23,700 --> 00:11:27,420
+Stryptomyces nodosis Amphotericin B Stryptomyces
+
+149
+00:11:27,420 --> 00:11:32,620
+Venezuella Cloramfenicol Stryptomyces Erythracines
+
+150
+00:11:32,620 --> 00:11:40,500
+Stryptomyces Erythrias Erythromycin Stryptomyces
+
+151
+00:11:40,500 --> 00:11:44,700
+Ephradiei Neomycin Stryptomyces Grecius
+
+152
+00:11:44,700 --> 00:11:46,040
+Stryptomycin
+
+153
+00:11:49,980 --> 00:11:52,660
+لأ طلعوا ليه على التلتة اللي احنا ذاكرتهم اخر اشي
+
+154
+00:11:52,660 --> 00:11:56,320
+نيوميسين و Striptomycin و Gendamycin هدول التلتة
+
+155
+00:11:56,320 --> 00:12:00,580
+عائلة واحدة ك Antibiotics العائلة دلوقت هم اسمها
+
+156
+00:12:00,580 --> 00:12:06,020
+Aminoglycosides Aminoglycosides لكن لفظ المنتجات
+
+157
+00:12:06,020 --> 00:12:08,840
+من وين؟ هل من نفس العائلة؟ هل لقينا micro
+
+158
+00:12:08,840 --> 00:12:14,020
+monospora و لقينا اللي هو Striptomyces يبقى ..
+
+159
+00:12:14,020 --> 00:12:19,700
+بنقدر نقول انهالتصنيف تبع ال Antibiotics could be
+
+160
+00:12:19,700 --> 00:12:23,380
+chemical classification, aminoglycosides could be
+
+161
+00:12:23,380 --> 00:12:28,520
+ال origin تبعه من بكتيريا و لا من فطريات مثلا هل
+
+162
+00:12:28,520 --> 00:12:31,440
+هو باسع الطيف و لا ضيق الطيف؟ بكتيريا سايدر و لا
+
+163
+00:12:31,440 --> 00:12:34,960
+بكتيريا هو؟ static، لأن في تصنيفات متنوعة و
+
+164
+00:12:34,960 --> 00:12:37,820
+متعددة، همر عليهم واحد واحد إن شاء الله
+
+165
+00:12:41,110 --> 00:12:45,210
+خلاصة مايكرو موانس بورا تاكورا فنجاي سفارس بوريوم
+
+166
+00:12:45,210 --> 00:12:49,690
+بنسليوم جريسيو فولفام و بنسليوم نوتاتم طلع بنسليوم
+
+167
+00:12:49,690 --> 00:12:53,350
+جريسيو فولفام طلع جريسيو فولفام بيه اللي هو Anti
+
+168
+00:12:53,350 --> 00:12:57,390
+-Fungal بينما بنسليوم نوتاتم طلع اللي هو ال
+
+169
+00:12:57,390 --> 00:13:01,490
+بنسيلين اللي هو Anti-Bacterial لذلك مافيش قاعدة
+
+170
+00:13:01,490 --> 00:13:06,700
+تحكم مين بنتج مينantibacteria مرة بكتيريا تنتج
+
+171
+00:13:06,700 --> 00:13:10,580
+antifungal واحدة طبعا لإن هذا أصلا كلمة antibiotic
+
+172
+00:13:10,580 --> 00:13:12,720
+من هنا أنا نسيت أحكي من هنا كلمة antibiotic إجت
+
+173
+00:13:12,720 --> 00:13:18,140
+antibiotic قدت من ظاهر اسمها Antibiosis بيوسس حياة
+
+174
+00:13:18,140 --> 00:13:23,200
+Anti ضد الحياة فكان في ظاهر اللي هي ظاهرة إنه
+
+175
+00:13:23,200 --> 00:13:29,680
+البكتيريا في في التربة لا توجد بشكل منفرد وإنها
+
+176
+00:13:29,680 --> 00:13:33,170
+توجد بشكل communitiesفاكرين لما قولنا competition
+
+177
+00:13:33,170 --> 00:13:37,690
+مرة مصطلح كتير مهم و منافسة ان وجود بكتيريا بتنافس
+
+178
+00:13:37,690 --> 00:13:41,810
+بتحدي طب كيف البكتيريا بتنافس؟ بعض على البعض أحد
+
+179
+00:13:41,810 --> 00:13:45,890
+أدايات المنافسة is to produce compounds that kills
+
+180
+00:13:45,890 --> 00:13:49,750
+others غير ان ناخد الحديد مصبوح فكرا الحديد او
+
+181
+00:13:49,750 --> 00:13:53,410
+نتنافس على نوترات معينة ممكن كمان ننتج ملكمات لقطع
+
+182
+00:13:59,190 --> 00:14:04,570
+طلعولي على الاطار اللي باللون الأزرق اللي بيقول
+
+183
+00:14:04,570 --> 00:14:07,670
+micro bacteria, ground negative bacteria, ground
+
+184
+00:14:07,670 --> 00:14:10,990
+positive bacteria, chlamydia, fungi, protozoa,
+
+185
+00:14:11,050 --> 00:14:14,410
+helminthes and viruses هذه هي ال micro organisms
+
+186
+00:14:14,410 --> 00:14:17,490
+ولا في شيء جديد أنكوا، هي ال micro organisms اللي
+
+187
+00:14:17,490 --> 00:14:20,990
+بيسميها micro organisms طلعولي على ال tetracycline
+
+188
+00:14:21,870 --> 00:14:24,730
+تترا ساكلين تأثيره على مين؟ على الـ ground
+
+189
+00:14:24,730 --> 00:14:27,150
+negative، على الـ ground positive، وعلى الـ
+
+190
+00:14:27,150 --> 00:14:31,470
+chlamydia كان مدرسنا الله يرحمه أكيد توفي كان
+
+191
+00:14:31,470 --> 00:14:35,130
+بيقول إذا ذهبت بالامتحان على بكتيريا و Antibiotic
+
+192
+00:14:35,130 --> 00:14:40,130
+من ال drug of choice حطه تترا ساكلين بنسبة 80%
+
+193
+00:14:40,130 --> 00:14:43,690
+هيكون الجوابق صحيح لأن لو اللحظة ال range بتاعه
+
+194
+00:14:46,930 --> 00:14:51,170
+البيانات زي الفنسالين it is وعادة في نرو نرو رانج
+
+195
+00:14:51,170 --> 00:14:55,870
+بس على الإجراءات مستر بكتيريا بينما ال .. ال .. ال
+
+196
+00:14:55,870 --> 00:14:58,510
+.. الستربتوميسين أثر على الميكو بكتيريا و أثر على
+
+197
+00:14:58,510 --> 00:15:02,050
+الإجراءات نيجاتية هناك ال ISO ميازات فقط على
+
+198
+00:15:02,050 --> 00:15:05,950
+الميكو بكتيريا إذا نقدر نقسم البكتيريا إلى قسمين
+
+199
+00:15:05,950 --> 00:15:11,130
+رئيسيا واحد اللي هو broad spectrum ويسعى الطريف و
+
+200
+00:15:11,130 --> 00:15:18,180
+narrow spectrum والسؤال اللي لازم أسأله مين أحسن؟و
+
+201
+00:15:18,180 --> 00:15:23,980
+نفس الغلطات اللي من نسخه المرضى، أنا قلت الجواب من
+
+202
+00:15:23,980 --> 00:15:25,500
+مبارزنا، الجواب دي من مبارزنا
+
+203
+00:15:29,950 --> 00:15:34,450
+بفضل بفضل ال neuro spectrum لما أكون عارف لما أكون
+
+204
+00:15:34,450 --> 00:15:37,910
+عارف بفضل ال neuro spectrum لما أكون مش عارف و
+
+205
+00:15:37,910 --> 00:15:42,550
+الحالة خطيرة مابتستناش من injustice مثلا نستخدم ال
+
+206
+00:15:42,550 --> 00:15:45,590
+broad spectrum إذا it really depends ورمه هذا
+
+207
+00:15:45,590 --> 00:15:50,750
+موجود و هذا موجود و يجب علينا أن نعلم متى نوظف كل
+
+208
+00:15:50,750 --> 00:15:55,110
+واحدة بالضبط نفس السؤال هنسأله bactericidal ولا
+
+209
+00:15:55,110 --> 00:15:57,250
+bacterostatic مين أحسن؟
+
+210
+00:16:01,790 --> 00:16:05,330
+إذا كان في الدم فورمينت سبتسيميا، يعني سبتسيميا
+
+211
+00:16:05,330 --> 00:16:08,370
+بشكل كبير، لأ نفضّل الـbacteriostatic لأن الصحيح
+
+212
+00:16:08,370 --> 00:16:11,150
+ده هيقدّي إلى الـGram Negative Septic Shock اللي
+
+213
+00:16:11,150 --> 00:16:15,730
+حكينا عنه، يبقى بدنا نعرف أنه Broad Spectrum و
+
+214
+00:16:15,730 --> 00:16:18,310
+Narrow Spectrum، على أي أساس صدفناهم Broad و
+
+215
+00:16:18,310 --> 00:16:24,230
+Narrow؟ على هل التأثير واسع، المجال و اللي هتضير،
+
+216
+00:16:24,230 --> 00:16:24,710
+المجال
+
+217
+00:16:27,460 --> 00:16:30,440
+طبعا كلمة broad spectrum ايش بيقبلها قولنا؟ now ال
+
+218
+00:16:30,440 --> 00:16:32,660
+spectrum هي التعريف الموجود في مصطلح super
+
+219
+00:16:32,660 --> 00:16:37,300
+infection حقيقة التعريفه شويه يعني confusing بس
+
+220
+00:16:37,300 --> 00:16:39,660
+خلينا نوضحه ما تطلعوا على السبوك و لا تطلعوا على
+
+221
+00:16:39,660 --> 00:16:44,260
+الكلام اللي بنحكي لأن احنا بصير انها infection
+
+222
+00:16:44,260 --> 00:16:49,320
+طبيعي انه ناخد antibioticالان لو البكتيريا اللى
+
+223
+00:16:49,320 --> 00:16:52,620
+عملت ال infection الاولى استجابت ال antibiotics،
+
+224
+00:16:52,620 --> 00:16:57,660
+تمام؟ ال antibiotic هل بيقتل فقط البكتيريا المسببة
+
+225
+00:16:57,660 --> 00:17:01,000
+بال infection ولا بيقتل معاها مايكروبات اخرى؟ اللى
+
+226
+00:17:01,000 --> 00:17:04,620
+بيقتل اى مايكروب هو عنده قدرة على قتله، مالميزش
+
+227
+00:17:04,620 --> 00:17:07,860
+بين infection و مش infection، اى مايكروب موجود
+
+228
+00:17:07,860 --> 00:17:13,730
+بيحتك فيه بيقتله و بيقدر يقتل يقتلهيبقى اتوقع لو
+
+229
+00:17:13,730 --> 00:17:17,390
+انا باخد عندي infection ان عندي اتهاب في ال oral
+
+230
+00:17:17,390 --> 00:17:21,710
+cavity في ال led ده مثلا واخدت antibiotic الفن فيه
+
+231
+00:17:21,710 --> 00:17:27,370
+normal flora مات البكتيرا و وراق خفت ال led اتهاب
+
+232
+00:17:27,370 --> 00:17:31,730
+ال led تلالي اشي تاني زي و كان ده البكانس الفن صار
+
+233
+00:17:31,730 --> 00:17:39,110
+كله أبيض و حم و مزعج جدا و مؤلممش اللي سببه؟ ان
+
+234
+00:17:39,110 --> 00:17:42,290
+انا خدت antibiotic ل infection فصار في infection
+
+235
+00:17:42,290 --> 00:17:46,950
+تانية فهي بيسموها super infection بس واضح انه
+
+236
+00:17:46,950 --> 00:17:51,270
+عجبنا التعريف احنا تمام؟ ان infection هو infection
+
+237
+00:17:51,270 --> 00:17:54,070
+ناتج على ال blood blood فهي في الواقع الأولى هي ال
+
+238
+00:17:54,070 --> 00:17:58,150
+infection ناتج عن مين؟ عن استخدام ال antibiotic
+
+239
+00:17:58,150 --> 00:17:59,750
+غالبا احنا استخدام ال antibiotic عشان انا عايز
+
+240
+00:17:59,750 --> 00:18:00,850
+اصلا infection
+
+241
+00:18:11,690 --> 00:18:15,110
+الشيء الوحيد اللي اختلف ان احنا دخلنا انتياباتيك
+
+242
+00:18:15,110 --> 00:18:18,010
+قتل ال normal flora ففعب ال normal flora او
+
+243
+00:18:18,010 --> 00:18:21,010
+مايكروفات اخرى استطاعت في غياب ال normal flora
+
+244
+00:18:21,010 --> 00:18:25,390
+انها تعمل growth و تعمل infection مرة تانية اشهر
+
+245
+00:18:25,390 --> 00:18:29,390
+مثال عليها الادم النموذج ال clostridium difficile
+
+246
+00:18:29,390 --> 00:18:33,110
+normal flora of ventricular tract
+
+247
+00:18:39,130 --> 00:18:41,570
+وإذا عادة محدودة وماوصلش لـ Quorum Sensing، مش
+
+248
+00:18:41,570 --> 00:18:45,430
+هتمدج تكسين سبعتها، لو أخدنا antibiotic for
+
+249
+00:18:45,430 --> 00:18:48,190
+prolonged period أو بعض أنواع من ال antibiotic حتى
+
+250
+00:18:48,190 --> 00:18:50,670
+for short period، بصير فيه خلل في الموضوع في
+
+251
+00:18:50,670 --> 00:18:54,250
+الورقة، فبصير لها overgrowth، إذا صارت overgrowth،
+
+252
+00:18:54,250 --> 00:18:59,000
+يبقى صار فانديش الآنProduction لتكسين A وB تبعونها
+
+253
+00:18:59,000 --> 00:19:03,360
+وبتعمل condition سيء ب resistance ديارية، ديارية
+
+254
+00:19:03,360 --> 00:19:06,840
+بتتطور لفترة طويلة و condition بيسموه Antibiotic
+
+255
+00:19:06,840 --> 00:19:10,340
+-induced pseudomembraneous colitis ده نبع على أنه
+
+256
+00:19:10,340 --> 00:19:14,160
+الـ colitis هذه ناجم عن استخدام الـ Antibiotic
+
+257
+00:19:14,160 --> 00:19:17,580
+كلمة Antibiotic-induced يعني اللي سبب إنتاجها هو
+
+258
+00:19:17,580 --> 00:19:22,910
+الـ Antibiotic مصطلحين أخرين في اللوحةعندي بكتيري
+
+259
+00:19:22,910 --> 00:19:26,930
+سايدل وبكتيري بكتيري أستاتيك كوننا كالوس بكتيريا
+
+260
+00:19:26,930 --> 00:19:30,090
+وإنهم استجرارات البكتيريا إيش الفرق من اتنين من
+
+261
+00:19:30,090 --> 00:19:36,250
+ناحية عملية؟ من ناحية عملية إنه لو أزلنا البكتيري
+
+262
+00:19:36,250 --> 00:19:41,630
+سايدل بعد فترة من الزمن البكتيريا لن تعود للانقسام
+
+263
+00:19:41,630 --> 00:19:45,370
+بينما البكتيري أستاتيك لو أزلنا البكتيري أستاتيك
+
+264
+00:19:45,370 --> 00:19:48,970
+هذا بعد فترة بصير البكتيري اتن من أحسن
+
+265
+00:19:53,570 --> 00:19:58,190
+البكتيريو أستاتيك البكتيريو أستاتيك له يعني روناق،
+
+266
+00:19:58,190 --> 00:20:00,630
+إيش هو روناق طبعا؟ بتعرف .. بتعرف ال Vibrio
+
+267
+00:20:00,630 --> 00:20:03,670
+cholera اللي أشرحناها قبل المحاضرة التانية؟ ال
+
+268
+00:20:03,670 --> 00:20:07,790
+Vibrio cholera على خطورتها وعلى يعني بالعادية على
+
+269
+00:20:07,790 --> 00:20:11,670
+التترا سايكلين، التترا سايكلين، التترا سايكلين
+
+270
+00:20:11,670 --> 00:20:17,530
+وصفه بكتيري أستاتيك، بكتيريو أستاتيك، ومع ذلك very
+
+271
+00:20:17,530 --> 00:20:19,110
+effective، بتعرف الطعم؟
+
+272
+00:20:23,450 --> 00:20:28,010
+إلا إنه خطير المرض إلا إنه bacteriostatic وما
+
+273
+00:20:28,010 --> 00:20:31,750
+ننساش إنه فيه جهات اسمه جهاز مانعة احنا بنوقف نشاط
+
+274
+00:20:31,750 --> 00:20:34,830
+البكتيريا و بنخل الجهاز المانعة برضه يقوم بالدور
+
+275
+00:20:34,830 --> 00:20:38,510
+المانوت هذا
+
+276
+00:20:38,510 --> 00:20:43,270
+كارتون، كارتون حلو كتير، بوضح إن على الجهة دي
+
+277
+00:20:43,270 --> 00:20:46,570
+bacteriostatic ماخدهم أسرة مربطة، مظبوطة
+
+278
+00:20:46,570 --> 00:20:51,210
+البكتيريا، بينما البكتيري سهل مش عاملمافيش أسرة
+
+279
+00:20:51,210 --> 00:20:54,550
+خلاص هيبقى قاتلة على طول طبعا في أمثلة هنا زي ال
+
+280
+00:20:54,550 --> 00:20:57,430
+chloramphenicol الارتروميسين والكليمناميسين ال
+
+281
+00:20:57,430 --> 00:21:00,870
+cellophanomyces ترانميثوبريم وتترا ساكلينز هدولة
+
+282
+00:21:00,870 --> 00:21:04,010
+اللي بياخدوا أسرة اللي هم بكتيريه أستاتيك ع الجهة
+
+283
+00:21:04,010 --> 00:21:08,630
+المقابلة فينديل أمينوجليكوسايدس و ال bedalactam
+
+284
+00:21:08,630 --> 00:21:11,230
+-antibiotics و ال vancomycin اللي كونلونز هدولة
+
+285
+00:21:11,230 --> 00:21:15,490
+بيعملوا بكتيريه صحيح بقى هنذكر جملة هنا لازم ننتبه
+
+286
+00:21:15,490 --> 00:21:20,270
+إليها كويسكلمة bactericide أو bacteriostatic is a
+
+287
+00:21:20,270 --> 00:21:24,650
+matter of concentration يعني لو زدنا ال
+
+288
+00:21:24,650 --> 00:21:29,910
+bacteriostatic concentration ممكن تقدر ديها القتل
+
+289
+00:21:29,910 --> 00:21:34,610
+لكن ليش احنا وصلنا بعض ال antibiotic بإنها
+
+290
+00:21:34,610 --> 00:21:38,070
+bacteriostatic at the therapeutic dose الجرعه
+
+291
+00:21:38,070 --> 00:21:41,970
+علاجية اللي استخدمت في العلاج هي bacteriostatic
+
+292
+00:21:41,970 --> 00:21:46,370
+كلمة جرعه دوس concentration صح او لا؟و العكس كمان
+
+293
+00:21:46,370 --> 00:21:49,310
+بالنسبة لمن؟ لجهة .. لجهة .. ل .. ال .. ال .. ال
+
+294
+00:21:49,310 --> 00:21:52,550
+bactericide اللي كانت بالفعل هو bactericide عادية
+
+295
+00:21:52,550 --> 00:21:55,870
+تانية اتنين بتباهوا مع بعض و حقها لهم ان انا
+
+296
+00:21:55,870 --> 00:21:59,190
+اتباههم لو اقوله انا انديه كلهم من نوع واحد اللي
+
+297
+00:21:59,190 --> 00:22:02,250
+بقتلهم gram negative زي ال .. ال .. ال .. ال .. ال
+
+298
+00:22:02,250 --> 00:22:03,410
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+299
+00:22:03,410 --> 00:22:04,710
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+300
+00:22:04,710 --> 00:22:04,910
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+301
+00:22:04,910 --> 00:22:06,850
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+302
+00:22:06,850 --> 00:22:07,010
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+303
+00:22:07,010 --> 00:22:07,430
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+304
+00:22:07,430 --> 00:22:12,070
+.. ال .. ال .. ال .. ال .. ال .. ال ..
+
+305
+00:22:15,180 --> 00:22:18,220
+اللوحة الأهم على الإطلاق لانجب ابدأ فيها بدي ارجع
+
+306
+00:22:18,220 --> 00:22:23,420
+برجوع اتكلم عن ال antibiotics او ال antimicrobials
+
+307
+00:22:23,420 --> 00:22:26,180
+انا قولت قبل شوية انه penicillins وcephalosporins
+
+308
+00:22:26,180 --> 00:22:28,940
+و amino glycosides اسماء هذي الأسماء اللي هون
+
+309
+00:22:28,940 --> 00:22:32,840
+جيبناها هذي chemical classification يعني مش احنا
+
+310
+00:22:32,840 --> 00:22:36,000
+اللي سميناها الكيميائيين بس ال microbiologists هم
+
+311
+00:22:36,000 --> 00:22:38,840
+اللي سموا جهود ال spectrum غيره spectrum أما
+
+312
+00:22:38,840 --> 00:22:42,380
+الكيميائين هم اللي عطوا الأسماء للمراكبات لإنه كل
+
+313
+00:22:42,380 --> 00:22:47,750
+مراكب له صفات محددةإذا تصنيف المراكبات كيميائيا مش
+
+314
+00:22:47,750 --> 00:22:50,570
+منانا تصنيفهم بكتيري صادق من بكتيري صادق منانا
+
+315
+00:22:50,570 --> 00:22:53,150
+تصنيفهم broad spectral و narrow spectral منانا
+
+316
+00:22:53,150 --> 00:22:56,450
+التصنيف الأهم على الإطلاق اللي قدامه على السبورة
+
+317
+00:22:56,450 --> 00:23:02,350
+هو based on the site affected أو targeted يعني
+
+318
+00:23:02,350 --> 00:23:05,370
+عالميا بيشتغل ال .. ال .. ال antibiotic على ال
+
+319
+00:23:05,370 --> 00:23:09,210
+cell wall و لا بيشتغل على ال cell membrane و لا
+
+320
+00:23:09,210 --> 00:23:13,660
+على ال protein synthesisأو كـ Antimetabolites كم
+
+321
+00:23:13,660 --> 00:23:17,800
+كده اذا ذكرتها انا؟ خمسة ندوا واحد منهم بالترتيب
+
+322
+00:23:17,800 --> 00:23:22,260
+من بره جوا cell wall cell membrane خلينا نقول
+
+323
+00:23:22,260 --> 00:23:25,280
+protein synthesis نكليك أسد synthesis وفي
+
+324
+00:23:25,280 --> 00:23:30,560
+antimetabolites antimetabolites يعني it inhibits a
+
+325
+00:23:30,560 --> 00:23:34,480
+certain metabolic step في الخلية عدى الأربع اللي
+
+326
+00:23:34,480 --> 00:23:41,850
+احنا ذكرناهم الأخرين تعالوا نطلع على ال cellCell
+
+327
+00:23:41,850 --> 00:23:46,170
+wall inhibitions of cell wall synthesis الانبجي
+
+328
+00:23:46,170 --> 00:23:49,470
+معناه اللي حاكى اي antibiotic او antimicrobial
+
+329
+00:23:49,470 --> 00:23:52,210
+اللي هو علاقة بال cell wall inhibition بصنفه تحت
+
+330
+00:23:52,210 --> 00:23:54,870
+مين cell wall inhibitors cell membrane inhibitors
+
+331
+00:23:54,870 --> 00:23:57,830
+protein synthesis inhibitors صلاة تصنيف جديد صلاة
+
+332
+00:23:57,830 --> 00:24:01,510
+تصنيف جديد و غالبا هو اللي احنا ليش التصنيف هذا
+
+333
+00:24:01,510 --> 00:24:05,230
+بهمنا لأن هو اللي بوضّحلك ال mechanism of action
+
+334
+00:24:05,230 --> 00:24:10,440
+تبع المركباتالـ Cephalosporins و الـ Penicillins
+
+335
+00:24:10,440 --> 00:24:13,880
+هم الانهيبترات يبقى ال chemical classification ايه
+
+336
+00:24:13,880 --> 00:24:17,420
+جبناها و حطيناها تحت تصنيع السل اللي هو ال
+
+337
+00:24:17,420 --> 00:24:23,240
+vancomycin-cadaric العمل تحت هنا injury to plasma
+
+338
+00:24:23,240 --> 00:24:25,400
+membrane اللي هو السل من برا طبعا السل من برا من
+
+339
+00:24:25,400 --> 00:24:29,460
+برا هو نفس السل من برا زي بوليميكسين B و ال
+
+340
+00:24:29,460 --> 00:24:34,640
+protein synthesis الجهة دي هما دولة ال protein
+
+341
+00:24:34,640 --> 00:24:37,990
+synthesisفي اندي فاكرين ال ال ال ال ال ال ال ال ال
+
+342
+00:24:37,990 --> 00:24:38,390
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+343
+00:24:38,390 --> 00:24:39,930
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+344
+00:24:39,930 --> 00:24:40,770
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+345
+00:24:40,770 --> 00:24:48,670
+ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+346
+00:24:48,670 --> 00:24:51,730
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+347
+00:24:51,730 --> 00:24:53,430
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+348
+00:24:53,430 --> 00:24:53,450
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+349
+00:24:53,450 --> 00:24:53,670
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+350
+00:25:06,700 --> 00:25:09,900
+أتوقع منكوا تحضروا تحضروا نوكليك أسد سينتسيز
+
+351
+00:25:09,900 --> 00:25:12,040
+نوكليك أسد نوكليك أسد نوكليك أسد نوكليك أسد نوكليك
+
+352
+00:25:12,040 --> 00:25:12,660
+أسد نوكليك أسد نوكليك أسد نوكليك أسد نوكليك أسد
+
+353
+00:25:12,660 --> 00:25:13,320
+نوكليك أسد نوكليك أسد نوكليك أسد نوكليك أسد نوكليك
+
+354
+00:25:13,320 --> 00:25:13,940
+أسد نوكليك أسد نوكليك أسد نوكليك أسد نوكليك أسد
+
+355
+00:25:13,940 --> 00:25:22,640
+نوكليك أسد نوكليك أسد نوكليك أسد نوكليك أسد
+
+356
+00:25:22,950 --> 00:25:27,210
+ماتحفظهم مش، بس هفرهم و خلاص، لأ؟ ليش؟ لأن بدون
+
+357
+00:25:27,210 --> 00:25:32,010
+معرفة ال process نفسها صعب عليا أن اوصلكم فكرة كيف
+
+358
+00:25:32,010 --> 00:25:34,650
+كل step أو كل antibody لأن أنا قلت protein
+
+359
+00:25:34,650 --> 00:25:37,910
+synthesis ما أنا حاول اقوله على طول، دخل في
+
+360
+00:25:37,910 --> 00:25:41,510
+أدمغاتنا إنهم كلهم بيشتغلوا بنفس الطريقة، لأ، هي
+
+361
+00:25:41,510 --> 00:25:46,170
+المحصلة النهائية إن ال protein synthesis وقف، لكن
+
+362
+00:25:46,170 --> 00:25:49,640
+كل واحد مناللي انا بكرته فدولة بيشتغل بطريقة
+
+363
+00:25:49,640 --> 00:25:52,780
+مختلفة تانية و لازم نكون عارفين كل واحدة منهم مش
+
+364
+00:25:52,780 --> 00:25:56,700
+هنقدر نفهم الكلمة ماذا إلا إذا استرجعنا ل protein
+
+365
+00:25:56,700 --> 00:26:00,140
+synthesis أو ال cell wall synthesis دي
+
+366
+00:26:11,430 --> 00:26:16,550
+أعتقد أن ال mRNA اللي موجود تحت هذا على ال .. زي
+
+367
+00:26:16,550 --> 00:26:21,150
+ما هقشر الآن بالماوس هذا ال RNA إذا بتلاحظوا في
+
+368
+00:26:21,150 --> 00:26:25,690
+عندي chloramphenicol, erythromycin, tetracycline و
+
+369
+00:26:25,690 --> 00:26:27,110
+streptomycin
+
+370
+00:26:30,650 --> 00:26:34,070
+بتعرف تماما عن بعضها البعض كيميائيا يعني ال
+
+371
+00:26:34,070 --> 00:26:39,210
+stripomycin بيمثل عائلة اسمها aminoglycosides فيها
+
+372
+00:26:39,210 --> 00:26:42,430
+أكتر من عشرين antibiotics كلهم بيشتغلوا بالنفس
+
+373
+00:26:42,430 --> 00:26:44,870
+الطريقة تبع ال stripomycin aminoglycosides
+
+374
+00:26:44,870 --> 00:26:48,790
+بيشتغلوا على مين؟ على ال 30 أسرعي بوزوه مرساليونية
+
+375
+00:26:48,790 --> 00:26:53,010
+بيعملوا bulging bulging يعني نوع من النتوء لما
+
+376
+00:26:53,010 --> 00:26:55,890
+بيجي الإمارة ان هي بدل من يكون مستوي بيصير فارق
+
+377
+00:26:56,180 --> 00:27:00,300
+بيفرض ال nucleotides طب ال anticonon كيف بترتبط
+
+378
+00:27:00,300 --> 00:27:05,480
+فيه؟ لحظة ال anticonon مصمم انه يرتبط ب
+
+379
+00:27:05,480 --> 00:27:10,220
+confirmation محددة،الان ال confirmation اتغيرت مش
+
+380
+00:27:10,220 --> 00:27:14,060
+هقدر انا اترتبط وكل اشي في ال antibiotic very
+
+381
+00:27:14,060 --> 00:27:17,840
+specific عشان هيك قلتلكوا البكتيريا قادرة على
+
+382
+00:27:17,840 --> 00:27:22,800
+تكوين resistance بسهولة لل antibiotic لان it is
+
+383
+00:27:22,800 --> 00:27:24,000
+targeting
+
+384
+00:27:27,130 --> 00:27:33,110
+يعني مثلا البكتيريا غيرت .. هو اين برتبط ال .. ال
+
+385
+00:27:33,110 --> 00:27:36,070
+.. ال .. الستراتومايسين برتبط في لزوغ معين في ال
+
+386
+00:27:36,070 --> 00:27:40,210
+30S لو صار في modification بسيط في هذا اللزوغ
+
+387
+00:27:40,210 --> 00:27:43,330
+المستقبل للاستراتومايسين بيبطل الاستراتومايسين
+
+388
+00:27:43,330 --> 00:27:45,910
+يرتبط في الريبوزام وبالتالي بصير البكتيريا
+
+389
+00:27:45,910 --> 00:27:50,350
+resistant يعني يبقى اي change طفيف
+
+390
+00:27:54,400 --> 00:27:58,380
+أكرة الباب، قلنا بنفتح الباب من الأكرة، من إيد
+
+391
+00:27:58,380 --> 00:28:03,360
+الباب، الآن هي ال target تبعنا للولوج إلى الغرف أو
+
+392
+00:28:03,360 --> 00:28:06,940
+للخروج، بمعنى إنه هي ال target تبعنا، زي ما ال
+
+393
+00:28:06,940 --> 00:28:11,560
+antibiotic له target، بلّت أكرة، بس بلّت المسافر
+
+394
+00:28:11,560 --> 00:28:14,480
+اللي ما كانت بينها و بين الباب، 2، 3، 19، حين حطنا
+
+395
+00:28:14,480 --> 00:28:18,540
+إيدينا فيها، لزجناها بالباب، بقى إلا أكرة، و بتقول
+
+396
+00:28:18,540 --> 00:28:21,020
+ما نفس الوضع، فتفتح و هتخلق الباب، لكن أنا مش هقدر
+
+397
+00:28:21,020 --> 00:28:24,380
+أحط إيدي و أمسك و أقنع الأكرةيبقى لما يتغير ال
+
+398
+00:28:24,380 --> 00:28:28,160
+target كمان لما يتغير ال target ال antibiotic
+
+399
+00:28:28,160 --> 00:28:32,380
+بيفشل في انه يقوم بوظيفته من هنا تبدأ عمالة اللي
+
+400
+00:28:32,380 --> 00:28:35,040
+هو ال resistance طبعا ال bacteria زي ما تقولي
+
+401
+00:28:35,040 --> 00:28:37,860
+دكاترة بدرسوكوا microbiology يبدو ان هم دكاترة
+
+402
+00:28:37,860 --> 00:28:41,920
+برضه بدرسوهم كيف يقاوموا يعني زي ما احنا بنخطط هم
+
+403
+00:28:41,920 --> 00:28:46,080
+برضه كمان بيخطط وكل ما بنكتشف antibiotic انا قولت
+
+404
+00:28:46,080 --> 00:28:51,160
+في ملايات المحاضرة الانسان ضن انه عنده يعني اكتر
+
+405
+00:28:51,160 --> 00:28:52,160
+من كفايته من
+
+406
+00:29:00,300 --> 00:29:05,080
+the last bullet مصبوع و هذا للأسف يعني كبار مرة
+
+407
+00:29:05,080 --> 00:29:09,580
+لسوء استخدام و سوء توظيف اللي هو المضادات الحاجة
+
+408
+00:29:09,580 --> 00:29:14,500
+أنا ده أقول مثال بقول بالألفين و خمسة عملنا دراسة
+
+409
+00:29:14,500 --> 00:29:20,560
+على أنت بنتك اسمه ميرو بنيم ميرو بنيم بيوصف بإنه
+
+410
+00:29:20,560 --> 00:29:23,240
+the proudest هو الأول
+
+411
+00:29:27,980 --> 00:29:31,380
+وبالفعل في ال 2005 لما عزلنا البكتيريا من مستشفى
+
+412
+00:29:31,380 --> 00:29:33,760
+الشفاء اللى هو المفروض انه resistant يعني ولا
+
+413
+00:29:33,760 --> 00:29:36,360
+بكتيريا كانت resistant للميروبنين كلهم sensitive
+
+414
+00:29:36,360 --> 00:29:40,080
+كل اللى عزلناهم كانوا sensitive للميروبنين بعد أقل
+
+415
+00:29:40,080 --> 00:29:43,620
+من 3 سنوات او 4 سنوات قمنا نعمل دراسة شبيهة واخد
+
+416
+00:29:43,620 --> 00:29:48,080
+لعيلات من مستشفى الشفاء بلغة تظهر ان 3% 5% 7%
+
+417
+00:29:48,080 --> 00:29:52,320
+resistance للميروبنين هذا مؤشر على ان احنا يعني ..
+
+418
+00:29:52,320 --> 00:29:55,480
+يعني خلال .. اذا كانت أقل من 3 سنوات صار لنا هذا
+
+419
+00:29:55,480 --> 00:29:59,360
+ال percentageتتوقع كله .. خد بالك .. الزيادة
+
+420
+00:29:59,360 --> 00:30:03,280
+متكونش تلاتة .. ستة .. تسعة .. لأ الزيادة بتكون
+
+421
+00:30:03,280 --> 00:30:07,280
+ممكن قفزات .. ممكن تكون قفزات وبالتالي بتوقع انه
+
+422
+00:30:07,280 --> 00:30:10,600
+خلال اقل من تلتة اربع سنوات برضه الملابنين يصيبوا
+
+423
+00:30:10,600 --> 00:30:13,720
+زي وزي لهم مكسلين او لانبسلين القديم اللي .. اللي
+
+424
+00:30:13,720 --> 00:30:19,140
+زمان البكير قامت لسوء الاستخدام .. لسوء الاستخدام
+
+425
+00:30:19,140 --> 00:30:21,560
+.. وسوء الاستخدام اللي هو أشكال متنوعة ممكن نتكلم
+
+426
+00:30:21,560 --> 00:30:26,350
+عنها في نهاية المحاضرة القادمةالنهاية المحاضرة
+
+427
+00:30:26,350 --> 00:30:29,650
+القادمة هتكون فيها اكتر من شغل انا قولتلكوا
+
+428
+00:30:29,650 --> 00:30:32,450
+هتقعدوا هتفرجوا هتكونوا اتفرجتوا في الفيديوهات
+
+429
+00:30:32,450 --> 00:30:36,070
+وانا هشرح اندي انيمايشن برضه توضح ال mechanism of
+
+430
+00:30:36,070 --> 00:30:40,150
+action لبعض ال antibiotics وهتطرق ل how to test
+
+431
+00:30:40,150 --> 00:30:43,670
+for the sensitivity of this antibiotic وما هي
+
+432
+00:30:43,670 --> 00:30:48,590
+الطرق اللي بتستخدمها البكتيريا في مقاومة الموضوع
+
+433
+00:30:48,590 --> 00:30:55,800
+الحيوانيأحنا اتفضنا أنه تصنيف الأساسي عنا كان مبني
+
+434
+00:30:55,800 --> 00:30:58,780
+على ال site affected اللي هو ال cell wall أو ال
+
+435
+00:30:58,780 --> 00:31:00,900
+cell membrane حتتبقى فيه أول مجموعة اللي هي
+
+436
+00:31:00,900 --> 00:31:04,640
+inhibitors of cell wall synthesis اللي منها ال
+
+437
+00:31:04,640 --> 00:31:09,520
+penicillin اللي قسمناه لقسمين natural penicillin و
+
+438
+00:31:09,520 --> 00:31:13,120
+semi-synthetic penicillin بما أن أنا مشاركتكم
+
+439
+00:31:13,120 --> 00:31:19,500
+وتفاعلكم لما أنا عندي penicillin natural لماذا قد
+
+440
+00:31:19,500 --> 00:31:24,700
+أرغبفي تحويله إلى او اعمله modification، بتعمله
+
+441
+00:31:24,700 --> 00:31:31,640
+تحوير او تطويره عشان
+
+442
+00:31:31,640 --> 00:31:40,060
+احسن منه، يعني مقاومة اكتر، زيادة الفعاليات، ايش
+
+443
+00:31:40,060 --> 00:31:42,980
+كمان؟ اللي ممكن انكبتله لإن في الدنياكيف يعني
+
+444
+00:31:42,980 --> 00:31:48,520
+الكمية؟ يعني بدل .. بدل ما أنتش انا طول ان أنتش
+
+445
+00:31:48,520 --> 00:31:51,860
+طول ان انا اعمل مثلا؟ او يكون sensitive اكتر يعني
+
+446
+00:31:51,860 --> 00:31:58,060
+انا نفس الاشي فعالي ايش كده؟ مثلا كمية مثلا أقل من
+
+447
+00:31:58,060 --> 00:32:01,700
+.. أقل من .. sight effects اقل من sight effects
+
+448
+00:32:01,700 --> 00:32:08,560
+مثلا طبعا ايش كده؟ يعني فعالي فعالي معاكطب انا ..
+
+449
+00:32:08,560 --> 00:32:11,880
+انا .. انا .. انا سأل سؤال يعني يمكن يكون في سبب
+
+450
+00:32:11,880 --> 00:32:16,000
+برضه، لو احنا مرضنا باللوازم، نفضل ناخد إبارح ولا
+
+451
+00:32:16,000 --> 00:32:20,240
+ناخد حبوب؟طيب يعني ممكن انا اعمل modification ل
+
+452
+00:32:20,240 --> 00:32:22,980
+drug عشان اغير الشكل الدوائي تبعه و الجرعه ليش
+
+453
+00:32:22,980 --> 00:32:25,020
+هتغيره؟ لازم يوضعوا إفره أصلا و لا يوضعوش أثبوب
+
+454
+00:32:25,020 --> 00:32:31,060
+البنسالين لأنه لا ينطص في الأمعاء لأ مش انهم يحبوا
+
+455
+00:32:31,060 --> 00:32:35,880
+ع زهورهم لأ لإن هو لا ينطص في الأمعاء فلو غيرنا و
+
+456
+00:32:35,880 --> 00:32:38,040
+أحنا بالفعل غيرنا و أخدنا ال unbicillin و
+
+457
+00:32:38,040 --> 00:32:41,120
+الamoxicillin مشتقات من البنسالين نفسنا ناخدهم
+
+458
+00:32:41,120 --> 00:32:47,210
+كبسولات all لإن التعديل صار قابل للانتصاصلكن ال
+
+459
+00:32:47,210 --> 00:32:50,690
+anbicillin بناخده أربع حتبات في اليوم و ال
+
+460
+00:32:50,690 --> 00:32:53,390
+amoxicillin بناخده تلت حتبات في اليوم عارفين ليش؟
+
+461
+00:32:53,390 --> 00:32:56,390
+لإن برضه ال absorbability ذات ال .. ال amoxicillin
+
+462
+00:32:56,390 --> 00:33:00,310
+أحسن من مين؟ من ال anbicillin فهناك طرح فتاخده
+
+463
+00:33:00,310 --> 00:33:04,410
+أربع مرات أو تاخده تلت مرات يبقى كمان .. شوف ال ..
+
+464
+00:33:04,410 --> 00:33:09,110
+ال .. ال modification انت عارفين أفضل drug .. أفضل
+
+465
+00:33:09,110 --> 00:33:12,050
+drug إنه ناخده مرة واحدة في .. مرة واحدة و .. و
+
+466
+00:33:12,050 --> 00:33:16,720
+خلاص ماخدوش بعدين حتى كمانبعد عشر أهلكم مصطلح اسمه
+
+467
+00:33:16,720 --> 00:33:21,180
+patient convenience patient convenience و patient
+
+468
+00:33:21,180 --> 00:33:26,440
+compliance convenience و compliance convenient رضا
+
+469
+00:33:26,440 --> 00:33:33,180
+للمريض satisfaction تبع المريض بوجه بوجهش طبعا مهم
+
+470
+00:33:33,180 --> 00:33:36,460
+هذا مهم مهم مهم في ال compliance ايش هي ال
+
+471
+00:33:36,460 --> 00:33:42,080
+compliance مدى التزام للمريضأنا كتبت وصفة طبية
+
+472
+00:33:42,080 --> 00:33:47,660
+لمريض تلت قبر على مدار تلت أيام كل يوم قبر الصبح
+
+473
+00:33:47,660 --> 00:33:52,520
+هذا المريض بلا أدم إله حياة و هصحى إنه لأ بيقدر وع
+
+474
+00:33:52,520 --> 00:33:57,140
+الشغل و حتى عنده tonsillitis بطلع من رفح الساعة
+
+475
+00:33:57,140 --> 00:34:00,760
+سبعة بيجي بيصل الجامعة تمانية بروح رفح الساعة خمسة
+
+476
+00:34:00,760 --> 00:34:04,440
+عشان ياخد إيه بلاوة بده يروح بده يروح على ممرض أو
+
+477
+00:34:04,440 --> 00:34:06,600
+على أيادة أو على .. مش هيعملها في البيت صح ده
+
+478
+00:34:06,600 --> 00:34:08,800
+فبتاقوا مؤلمة بقولك
+
+479
+00:34:13,160 --> 00:34:15,920
+ما هو compliance؟ مش compliance، لكن لو كانت
+
+480
+00:34:15,920 --> 00:34:18,780
+كابسولة بدل ال injection، طب ما هو بياخدها في أي
+
+481
+00:34:18,780 --> 00:34:21,760
+وقت فرار بين محاضرة ومحاضرة، مال يبقى فيه كاس ميه
+
+482
+00:34:21,760 --> 00:34:24,920
+وبيشتغل ويحضر؟ لأ، يبقى ال patient convenience
+
+483
+00:34:24,920 --> 00:34:26,880
+يؤدي إلى ال patient compliance
+
+484
+00:34:31,390 --> 00:34:34,790
+جزء من الهدايا فشل، ابقى عايز أحسن فيه انتكاسة،
+
+485
+00:34:34,790 --> 00:34:38,410
+وفي نفس الواجهة، تذكروا إن أنا فيها بكتيري يهتكت
+
+486
+00:34:38,410 --> 00:34:41,990
+بالـ antibiotic بـconcentration لم تصل إلى فترة
+
+487
+00:34:41,990 --> 00:34:43,770
+طويلة تقتل البكتيري وقتها، اللي ممكن تكون
+
+488
+00:34:43,770 --> 00:34:44,730
+resistance
+
+489
+00:34:50,700 --> 00:34:53,880
+بيقدّي ل compliance هذا الكلام مفهوم في المستشفى
+
+490
+00:34:53,880 --> 00:34:56,700
+لو أنا نايم في المستشفى و معالجي اللي محلول يعطوني
+
+491
+00:34:56,700 --> 00:34:58,620
+انجليش مش مش مش مش مش مش مش مش مش مش مش مش مش مش
+
+492
+00:34:58,620 --> 00:34:59,500
+مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش
+
+493
+00:34:59,500 --> 00:34:59,520
+مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش
+
+494
+00:34:59,520 --> 00:34:59,940
+مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش
+
+495
+00:34:59,940 --> 00:35:00,040
+مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش
+
+496
+00:35:00,040 --> 00:35:02,560
+مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش
+
+497
+00:35:02,560 --> 00:35:02,880
+مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش
+
+498
+00:35:02,880 --> 00:35:04,680
+مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش
+
+499
+00:35:04,680 --> 00:35:06,580
+مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش
+
+500
+00:35:06,580 --> 00:35:17,060
+مش مش مش
+
+501
+00:35:17,060 --> 00:35:21,780
+مش مش مشلأسباب متلوعة ذكرته مجموعة منها وعلى ما
+
+502
+00:35:21,780 --> 00:35:24,760
+أنصح انه ندور على أسباب أخرى كمان نفكر في أسباب
+
+503
+00:35:24,760 --> 00:35:31,200
+أخرى منها ال .. ال .. ال .. to extend the spectrum
+
+504
+00:35:31,200 --> 00:35:35,260
+منها to combat resistance منها to improve quality
+
+505
+00:35:35,260 --> 00:35:38,620
+.. to remove side effects to be more safer زي ما
+
+506
+00:35:38,620 --> 00:35:43,180
+حكينا أو لتغيير الجرعة الدوائية من injection إلى
+
+507
+00:35:43,180 --> 00:35:47,780
+مثلا ولا إلا آخرلذلك ضغط عندي natural penicillin و
+
+508
+00:35:47,780 --> 00:35:52,300
+ضغط عندي semi-synthetic penicillin، semi-synthetic
+
+509
+00:35:52,300 --> 00:35:54,920
+معناها إنه أنا أخدت ال natural و عملتله
+
+510
+00:35:54,920 --> 00:36:00,380
+modification، مش completely synthetic، يعني ما
+
+511
+00:36:00,380 --> 00:36:03,880
+صنعت من السفر، أنتجنا بنسالين من الفطر و بعدين
+
+512
+00:36:03,880 --> 00:36:05,480
+أخدنا .. عملنا له modification
+
+513
+00:36:13,920 --> 00:36:17,580
+برضه كمان الكيميائيين اللى احنا بيشوفهم بيصنعوا
+
+514
+00:36:17,580 --> 00:36:23,040
+مركبات هدول علهم يعني ملكات خاصة في تخيل المركبات
+
+515
+00:36:23,040 --> 00:36:28,980
+وخصائصها فلعبوا مركب الأول قبل عن حرقة وجنبها CH2
+
+516
+00:36:28,980 --> 00:36:34,960
+وبعدين common nucleus نواة كيميائية مشتركة في كل
+
+517
+00:36:34,960 --> 00:36:39,000
+الفنسينات كل الفنسينات فيها نفس ال .. النواة هذه
+
+518
+00:36:39,810 --> 00:36:45,450
+ايش الافتلاف بين الأول والتاني CH2 والتاني OCH2؟
+
+519
+00:36:45,450 --> 00:36:49,550
+هداك الأولاني مستحيل الجسم يمتص منه milligram
+
+520
+00:36:49,550 --> 00:36:55,450
+والتاني بيمتص منه عادي، يعني تغير ال absorbance
+
+521
+00:36:55,450 --> 00:36:59,890
+تماما لل Antibiotic اللي اسمه Benzaline فقط بإضافة
+
+522
+00:36:59,890 --> 00:37:03,490
+Oxygen مدان ال CH2 اللي في ال .. في ال side chain
+
+523
+00:37:04,630 --> 00:37:09,010
+متخيلين ولا كيف؟ اذا اي صلعة ت change قد يؤدي الى
+
+524
+00:37:09,010 --> 00:37:12,830
+dramatic change في الخصائص المركبات مش هتقول الله
+
+525
+00:37:12,830 --> 00:37:17,710
+انه لذلك .. لذلك بنقول الكيميائين عندهم مقدرة على
+
+526
+00:37:17,710 --> 00:37:21,250
+إضافة درّة كلور .. تلت درّات كلور زي كلور
+
+527
+00:37:21,250 --> 00:37:26,410
+تترسيكلين تمام؟ ففي عندهم .. عندهم المقدرة على
+
+528
+00:37:26,410 --> 00:37:29,950
+تغيير المركبات وبتالي تغيير خصائص هذه المركبات
+
+529
+00:37:29,950 --> 00:37:33,230
+المشكلة ان في غزة احنا بنصنع مركبات حقيقة يعني انا
+
+530
+00:37:33,230 --> 00:37:40,750
+الكيميائين الأخوة الليهي قدرة تنافس قدرات العالم
+
+531
+00:37:40,750 --> 00:37:44,210
+كله في موضوع التصنيع لكن ماعناش مقدرة على انه نعمل
+
+532
+00:37:44,210 --> 00:37:49,710
+testing لها دي الملكبات مدى فعاليتها بشكل جيد في
+
+533
+00:37:49,710 --> 00:37:55,950
+عندي حاجة اسمها oxasilin وهو resistant to
+
+534
+00:37:55,950 --> 00:37:58,690
+penicillinase مكتوب على الانبوسين resistant to
+
+535
+00:37:58,690 --> 00:38:03,040
+penicillinase ايش ال penicillinase؟البنسيلينيز هو
+
+536
+00:38:03,040 --> 00:38:07,560
+الانزيم اللى بيكسر البنسيلين اتطلعوا على البنسيلين
+
+537
+00:38:07,560 --> 00:38:12,000
+يقولون ان هذى .. هذى .. اذا قولتها في مراقبتيها
+
+538
+00:38:12,000 --> 00:38:16,360
+فاسمها بنسيلين لكن هذى الجزء اللى هذا شايفينه اللى
+
+539
+00:38:16,360 --> 00:38:20,020
+هى قامنين انا نساهم عليها اسمها بيتالاكتم رينج
+
+540
+00:38:20,020 --> 00:38:25,980
+البيتالاكتم رينج ال بنسيلينيز بيستهدفها بما ال cut
+
+541
+00:38:25,980 --> 00:38:32,540
+فيها cutبصير البنسالين غير قادر على إحداث ال
+
+542
+00:38:32,540 --> 00:38:38,180
+action تبعه بالمرة، بفقد قدرته، تمام؟ يبقى ممكن
+
+543
+00:38:38,180 --> 00:38:43,320
+نسميه penicillinase أو بنسميه beta-lactamase عشان
+
+544
+00:38:43,320 --> 00:38:45,740
+ذلك ال antibiotics بيسموها beta-lactam-antibiotic
+
+545
+00:38:45,740 --> 00:38:48,700
+واحدة لحياة، ومعها بيسموها penicillins اللي اسم
+
+546
+00:38:48,700 --> 00:38:54,100
+الكيميا، إذن بنتخيل مع بعض كانت التالي إنه بكتيريا
+
+547
+00:38:54,100 --> 00:39:00,460
+أندجت penicillinase بيكسر البنسالينفاحنا حورنا ال
+
+548
+00:39:00,460 --> 00:39:02,720
+penicillin تبعنا و خلناه oxycelin بدل ما هو
+
+549
+00:39:02,720 --> 00:39:07,700
+penicillin عشان مايتكسرش، لكن البكتيريا وين؟ على
+
+550
+00:39:07,700 --> 00:39:10,280
+مين؟ على البكتيريا عندهم برضه و لما organic
+
+551
+00:39:10,280 --> 00:39:15,160
+chemistry أشتر من تبعوننا، طبعا؟ لذلك لما بنقول
+
+552
+00:39:15,160 --> 00:39:17,760
+penicillinase غالبا ما بنقولش penicillinase و
+
+553
+00:39:17,760 --> 00:39:22,020
+بنسكت، بنقول penicillinasesبمعنى ان البكتيريا
+
+554
+00:39:22,020 --> 00:39:26,720
+ستنتج أنواع مختلفة من ال Penicillinases عشان تكافح
+
+555
+00:39:26,720 --> 00:39:30,520
+كل Penicillin احنا نعمله Synthesis جديد هي برضه
+
+556
+00:39:30,520 --> 00:39:35,980
+بتطور من قدرتها و تنتج Penicillin الاخير في
+
+557
+00:39:35,980 --> 00:39:39,900
+القائمة اللي هو ال Anbicillin واضح بين بثانيه انه
+
+558
+00:39:39,900 --> 00:39:44,020
+Extended Spectrum بقى فقط المنسلين الرامبوسة بينما
+
+559
+00:39:44,020 --> 00:39:47,120
+ال Anbicillin بيستخدموله ال E.coli في ال .. في ال
+
+560
+00:39:47,120 --> 00:39:54,940
+urinary tract infection ال E.coli أشهيةيبقى فيها
+
+561
+00:39:54,940 --> 00:40:00,820
+أكتر من سبب للعب لللعب في المراكبات وتحويلها أو
+
+562
+00:40:00,820 --> 00:40:01,600
+تحويلها
+
+563
+00:40:06,120 --> 00:40:09,180
+في ال .. في ال .. في ال drugs مش على اكتشاف ال new
+
+564
+00:40:09,180 --> 00:40:12,680
+drugs و ان انا على modification لل existing drug
+
+565
+00:40:12,680 --> 00:40:17,040
+ليش؟ لأن ال existing drugs غالبا جرّبك و هي safe،
+
+566
+00:40:17,040 --> 00:40:22,240
+أه؟ فبالتالي ال modification قد تعدل .. تحسن بما
+
+567
+00:40:22,240 --> 00:40:24,900
+لا .. يعني في .. في .. في معروف الحياة
+
+568
+00:40:29,910 --> 00:40:34,630
+صعب الصورة هذه، اي واحد بيطلع فيها، يعني بيقول ان
+
+569
+00:40:34,630 --> 00:40:39,370
+ما بيسمها الصحيح، ماكانش يعني موفق، لإن لو ركز
+
+570
+00:40:39,370 --> 00:40:44,150
+فيها دقائق محدودة، ممكن يعرف منها بعض المعلومات،
+
+571
+00:40:44,150 --> 00:40:50,230
+اللي بنوع الأحمر، واحد، اتنين، ثلاثة، اربع
+
+572
+00:40:53,830 --> 00:40:57,710
+هما تسعة اصناف من ال antibiotics كلهم بيشتغلوا على
+
+573
+00:40:57,710 --> 00:41:04,510
+ال cell wall لكن كلهم بيشتغلوا على different steps
+
+574
+00:41:04,510 --> 00:41:10,670
+من ال cell wall برضه هذه نقطة مهمة انها زي ما قلت
+
+575
+00:41:10,670 --> 00:41:13,630
+لك في ال protein synthesis okay they inhibit
+
+576
+00:41:13,630 --> 00:41:15,850
+protein synthesis لكن برضه with different
+
+577
+00:41:15,850 --> 00:41:19,090
+mechanism of ايه of action كذلك بالنسبة لها ال
+
+578
+00:41:19,090 --> 00:41:24,180
+cellطيب هنبرخص الكلام اللي حكيناه Penicillin,
+
+579
+00:41:24,240 --> 00:41:26,920
+Penicillinase, Resistant Penicillins, Extended
+
+580
+00:41:26,920 --> 00:41:30,600
+Spectrum Penicillins الرابعة لأ ماذكرناهاش الرابعة
+
+581
+00:41:30,600 --> 00:41:34,740
+و هنشرحها لأ Penicillins زائد Beta-lactam, Beta
+
+582
+00:41:34,740 --> 00:41:38,400
+-lactams in it تلقوا معايا الآن على المثال اللي
+
+583
+00:41:38,400 --> 00:41:44,500
+بتحكيه هي البكتيريا و معها صيف ايش صيفها لأ ال
+
+584
+00:41:44,500 --> 00:41:50,460
+Beta-lactamaseعطينا المريض انتيباتك اسمه بنسالين
+
+585
+00:41:50,460 --> 00:41:54,540
+فالبكتيريا بتقول لبنسالين روح تعامل معاه، روح
+
+586
+00:41:54,540 --> 00:41:59,660
+تعامل معاه فال بنسالينيز بيكسر البنسالين، يبقي
+
+587
+00:41:59,660 --> 00:42:04,680
+البنسالين صار useless فالإنسان مش عامل، جاب
+
+588
+00:42:04,680 --> 00:42:09,780
+انتيباتك بنسالين و جاب معاه inhibitor للبنسالينيز
+
+589
+00:42:11,210 --> 00:42:13,370
+فصار ال Antibiotic لما يشوف البنسيلنايزي يقولله
+
+590
+00:42:13,370 --> 00:42:16,330
+الليه؟ يقولله ال .. ال .. ال .. ال inhibitor بتبقى
+
+591
+00:42:16,330 --> 00:42:19,830
+هو متعامل مع مين؟ مع ال .. ال .. ال enzyme
+
+592
+00:42:19,830 --> 00:42:22,450
+البنسيلنايزي هو صار neutral really مين البكتيريا
+
+593
+00:42:22,450 --> 00:42:29,030
+sensitive لبنسيلنايزي و أشهر drug انا في غزة مع
+
+594
+00:42:29,030 --> 00:42:31,670
+موظفكوا بتعرفوا اسمه Augmentin
+
+595
+00:42:36,140 --> 00:42:41,060
+من Amoxicillin زائد Clavunelic Acid ال Amoxicillin
+
+596
+00:42:41,060 --> 00:42:45,800
+هو Penicillin وال Clavunelic Acid هو Penicillinase
+
+597
+00:42:45,800 --> 00:42:50,200
+Inhibitor Penicillinase Inhibitor وبالتالي ارجع او
+
+598
+00:42:50,200 --> 00:42:54,480
+رجعنا حساسية ال Penicillin عن طريق بعض لذلك
+
+599
+00:42:54,480 --> 00:42:57,300
+بالنسبالي ان ال Augmentin is more expensive than
+
+600
+00:42:57,300 --> 00:43:00,240
+Amoxicillin a lot لأن بالفعل Amoxicillin غالب
+
+601
+00:43:00,240 --> 00:43:04,590
+الأوقات is useless تقريبابالطبع ليس كل الأحوال
+
+602
+00:43:04,590 --> 00:43:09,750
+بينما الـ augmentation is more effective وفي نقطة
+
+603
+00:43:09,750 --> 00:43:13,990
+أريد أن أركز عليها شائعة خطأ شائعة واللي للأسف
+
+604
+00:43:13,990 --> 00:43:18,470
+يعني اللي بيروح لهم الأطباء اللي هم يقولونك يبتدلك
+
+605
+00:43:18,470 --> 00:43:22,310
+أنت بيتك قوي لا يولد بلد حد اسمه أنت بيتك قوي و
+
+606
+00:43:22,310 --> 00:43:26,570
+أنت بيتك ضعيف يولد أنت بيتك مناسب و أنت بيتك غير
+
+607
+00:43:26,570 --> 00:43:33,470
+مناسب كلمة قاوميمريح على المريض، مريح على المريض
+
+608
+00:43:33,470 --> 00:43:37,410
+بيعطيه إنه لاخده الله مضال حياة قوي، لأ، لأ، لسه
+
+609
+00:43:37,410 --> 00:43:42,830
+للأسف، للأسف أساءت و سيئة استخدامها وبالتالي صاروا
+
+610
+00:43:42,830 --> 00:43:46,270
+يطلقوا على ال broad spectrum إنه إيه أنت بقيت
+
+611
+00:43:46,270 --> 00:43:51,070
+القوي، فصار المريض ياخد ال broad spectrum و يشفى
+
+612
+00:43:51,070 --> 00:43:53,210
+من المرض طبعه بسهولة و بسهولة
+
+613
+00:43:55,670 --> 00:43:58,350
+الاخرى نتيجة عن الخلل اللى حكينا عنه في ال normal
+
+614
+00:43:58,350 --> 00:44:02,730
+أفلام أستاذ بس هلأ الشىء اللى بيخلي البكتيريا تروح
+
+615
+00:44:02,730 --> 00:44:20,290
+لإنهيبترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمتر
+
+616
+00:44:21,290 --> 00:44:24,470
+ال .. ال .. ال inheritor هيرتبط بمين؟ لما .. لو ..
+
+617
+00:44:24,470 --> 00:44:27,890
+لو .. في مجموعة هيرتبط في .. في .. في mixture ال
+
+618
+00:44:27,890 --> 00:44:30,870
+.. ال inheritor هيرتبط بال باليزينايز و ال anthrac
+
+619
+00:44:30,870 --> 00:44:34,150
+هيرتبط بمين؟ بال بكتيريا بال بكتيريا بال بكتيريا
+
+620
+00:44:34,150 --> 00:44:38,170
+بال بكتيريا في كلمة class اسمه Carb Abnames و هذا
+
+621
+00:44:38,170 --> 00:44:41,250
+اللي قلت عنه قبل شوية انا اللي منه ال merabname
+
+622
+00:44:41,250 --> 00:44:45,740
+اللي هو the broadest spectrum of allالصورة طبعا
+
+623
+00:44:45,740 --> 00:44:49,080
+البنسالين والبنسالينيز شوفوا التكسير اللى صار فى
+
+624
+00:44:49,080 --> 00:44:52,400
+ال beta lactam ring فقط فى المنطقة دى صار بنسلوك
+
+625
+00:44:52,400 --> 00:44:55,440
+أسد او بنسلوك أسد فقط القدر على انه يعمل
+
+626
+00:44:55,440 --> 00:44:58,040
+inhibition للبرتيريا او killing للبرتيريا ونكمل ان
+
+627
+00:44:58,040 --> 00:44:59,320
+شاء الله الموضوع القادر نفس العملي
+
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/BMp05amsL4I_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/BMp05amsL4I_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..1ad97f28ca353e33f79a472e250e75011e71efaf
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/BMp05amsL4I_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 4577, "start": 20.79, "end": 45.77, "text": "بسم الله الرحمن الرحيم، الموضوع الجديد، بأنوان Antimicrobial Drugs، هنركز فيه بشكل كبير على ال mechanism of action لل drugs أو ال antibiotics إذا بتلاحظوا، احنا جادين بنتكلم، بنقول Antimicrobial و بنقول Antibiotics في نفس الوقت، و أكيد إنهم اتنين واحد، لكن واضح إن احنا بنتكلم عن مصطلحين مختلفين تماما، وبالتالي لنحاول", "tokens": [3555, 38251, 21984, 34892, 5016, 27842, 34892, 5016, 32640, 12399, 9673, 2407, 11242, 45367, 25724, 16254, 3215, 12399, 4724, 33456, 2407, 7649, 5130, 332, 299, 16614, 831, 2491, 14950, 12399, 8032, 1863, 31747, 11622, 8978, 3224, 4724, 8592, 28820, 9122, 3555, 13546, 15844, 2423, 7513, 295, 3069, 24976, 7766, 34051, 2423, 26922, 11933, 15730, 39894, 15040, 5016, 19913, 14407, 12399, 1975, 5016, 8315, 10874, 18513, 9957, 4724, 29399, 4117, 19528, 12399, 44945, 39648, 5130, 332, 299, 16614, 831, 4032, 44945, 39648, 5130, 897, 6471, 1167, 8978, 8717, 36178, 2423, 30543, 2655, 12399, 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0.333709716796875}, {"start": 2692.82, "end": 2693.28, "word": " بنسلوك", "probability": 0.97802734375}, {"start": 2693.28, "end": 2693.52, "word": " أسد", "probability": 0.9166666666666666}, {"start": 2693.52, "end": 2693.94, "word": " فقط", "probability": 0.936767578125}, {"start": 2693.94, "end": 2694.82, "word": " القدر", "probability": 0.7233072916666666}, {"start": 2694.82, "end": 2695.02, "word": " على", "probability": 0.8671875}, {"start": 2695.02, "end": 2695.24, "word": " انه", "probability": 0.683349609375}, {"start": 2695.24, "end": 2695.44, "word": " يعمل", "probability": 0.9713541666666666}, {"start": 2695.44, "end": 2695.84, "word": " inhibition", "probability": 0.6668701171875}, {"start": 2695.84, "end": 2696.34, "word": " للبرتيريا", "probability": 0.714013671875}, {"start": 2696.34, "end": 2696.48, "word": " او", "probability": 0.7012939453125}, {"start": 2696.48, "end": 2696.7, "word": " killing", "probability": 0.85546875}, {"start": 2696.7, "end": 2697.4, "word": " للبرتيريا", "probability": 0.96279296875}, {"start": 2697.4, "end": 2697.98, "word": " ونكمل", "probability": 0.9217529296875}, {"start": 2697.98, "end": 2698.04, "word": " ان", "probability": 0.8779296875}, {"start": 2698.04, "end": 2698.2, "word": " شاء", "probability": 0.916259765625}, {"start": 2698.2, "end": 2698.28, "word": " الله", "probability": 0.970703125}, {"start": 2698.28, "end": 2698.5, "word": " الموضوع", "probability": 0.84063720703125}, {"start": 2698.5, "end": 2698.78, "word": " القادر", "probability": 0.7975260416666666}, {"start": 2698.78, "end": 2698.98, "word": " نفس", "probability": 0.832275390625}, {"start": 2698.98, "end": 2699.32, "word": " العملي", "probability": 0.7001953125}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 2700.80575, "duration_after_vad": 2469.374999999991}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/BMp05amsL4I_raw.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/BMp05amsL4I_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..7be02cec7e3cc6e7834cb07fa0d095950d0db142
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/BMp05amsL4I_raw.srt
@@ -0,0 +1,2532 @@
+1
+00:00:20,790 --> 00:00:24,490
+بسم الله الرحمن الرحيم، الموضوع الجديد، بأنوان
+
+2
+00:00:24,490 --> 00:00:28,610
+Antimicrobial Drugs، هنركز فيه بشكل كبير على ال
+
+3
+00:00:28,610 --> 00:00:33,230
+mechanism of action لل drugs أو ال antibiotics إذا
+
+4
+00:00:33,230 --> 00:00:35,810
+بتلاحظوا، احنا جادين بنتكلم، بنقول Antimicrobial و
+
+5
+00:00:35,810 --> 00:00:38,950
+بنقول Antibiotics في نفس الوقت، و أكيد إنهم اتنين
+
+6
+00:00:38,950 --> 00:00:44,530
+واحد، لكن واضح إن احنا بنتكلم عن مصطلحين مختلفين
+
+7
+00:00:44,530 --> 00:00:45,770
+تماما، وبالتالي لنحاول
+
+8
+00:00:51,160 --> 00:00:54,100
+أنا مشترك في جزء كبير من التعريف، الجزء الأول من
+
+9
+00:00:54,100 --> 00:00:57,880
+التعريف ان they kill or inhibit microorganisms،
+
+10
+00:00:57,880 --> 00:01:02,060
+هذا متفق عليه، they kill or inhibit، و in small
+
+11
+00:01:02,060 --> 00:01:06,880
+concentration، يعني بتراجيز منخفضة جدا، لكن الفرق
+
+12
+00:01:06,880 --> 00:01:12,080
+بينهم ان انت بيتك في شرط لازم يكون naturalغالبا
+
+13
+00:01:12,080 --> 00:01:16,220
+كلمة natural source تعني انه .. انه .. انه مصطنبط
+
+14
+00:01:16,220 --> 00:01:19,220
+من بكتيريا او فطريات يعني محفوظ من بكتيريا او
+
+15
+00:01:19,220 --> 00:01:23,960
+فطريات بنما ال antimicrobial لم يشترط هذا الشرط
+
+16
+00:01:23,960 --> 00:01:28,520
+وبالتالي مين اعام ال antimicrobial اعام من ال
+
+17
+00:01:28,520 --> 00:01:32,920
+antibiotic فهيشبوب بقدر يقول بامان كل antibiotic
+
+18
+00:01:32,920 --> 00:01:37,240
+هو antimicrobial ولكن مضبط وليس كل antimicrobial
+
+19
+00:01:37,240 --> 00:01:42,540
+هو antibioticطبعا احنا كده مرة لا نقلل من اهمية
+
+20
+00:01:42,540 --> 00:01:45,580
+ذاكرة او هذا الاهمية التانية اللي هو استخداماتهم
+
+21
+00:01:45,580 --> 00:01:50,840
+ففي مثلا مراكزات سلفة مشهورة ومعروفة انها
+
+22
+00:01:50,840 --> 00:01:53,220
+synthetic مخلقة و هي تات ناتشران و هي
+
+23
+00:01:53,220 --> 00:01:57,720
+antimicrobial و هي safe و تستخدم استخدامها ردًا
+
+24
+00:01:57,720 --> 00:02:02,700
+ويلها يعني توظيف جيد في ال treatment المصطلح الأول
+
+25
+00:02:02,700 --> 00:02:06,760
+اللي على السطورة هو ال chemotherapyالكيموثيرابي هو
+
+26
+00:02:06,760 --> 00:02:11,720
+اي .. اي توظيف ل chemical drugs في ال treatment
+
+27
+00:02:11,720 --> 00:02:13,900
+طبعا هاجوله وقتها هوواش treatment ما هو chemical
+
+28
+00:02:13,900 --> 00:02:18,800
+drugs لأ فيه surgery صح؟ جراحة .. فيه natural
+
+29
+00:02:18,800 --> 00:02:23,580
+remedies، نباتات، فيه مساج، فيه تبليك .. لأ لكن
+
+30
+00:02:23,580 --> 00:02:28,440
+إذا موظفنا ال chemicals أو chemical drugs فيه،
+
+31
+00:02:28,440 --> 00:02:31,280
+احنا الشائعين عنها للأسف إنه يقولوا كيموثيرابي ليه
+
+32
+00:02:31,280 --> 00:02:36,180
+علاش كماوي، يعني كاسيلكن الواقع الأول هو توظيف ال
+
+33
+00:02:36,180 --> 00:02:40,060
+chemicals في ال treatments المصطلح الأخير هو
+
+34
+00:02:40,060 --> 00:02:43,820
+الأكثر أهمية على الإطلاق selective toxicity
+
+35
+00:02:43,820 --> 00:02:48,480
+بتعطيكم أسلامها قبل ما مقدمة قبل أسبوعين و أتلافة
+
+36
+00:02:48,480 --> 00:02:53,280
+نشرنا paper مع قسم الكيميا paper لنبحث هم صنعوا
+
+37
+00:02:53,280 --> 00:02:55,760
+بعض المركبات ال organic
+
+38
+00:02:59,870 --> 00:03:03,450
+هذه المراكبات انهم ينشروا هذه المراكبات انهم صنعوا
+
+39
+00:03:03,450 --> 00:03:07,430
+نوع جديد من المراكبات فانصحتهم و قلتلهم انه لأ
+
+40
+00:03:07,430 --> 00:03:12,290
+وقفه اللى بتصنعه ماتنشروش خلاص خدنا نعمله testing
+
+41
+00:03:12,290 --> 00:03:14,890
+نشوف هل هو antimicrobial activity و لا مش
+
+42
+00:03:14,890 --> 00:03:17,290
+antimicrobial activity و بالفعل لو ياله هو
+
+43
+00:03:17,290 --> 00:03:21,230
+antimicrobial activity الان نشرتنا ال paper اللى
+
+44
+00:03:21,230 --> 00:03:25,070
+هو البحث في مجلة عالمية محترمة بويسة و قبل البحث
+
+45
+00:03:25,070 --> 00:03:29,720
+انتشرتهل يمكن أستخدم مراكبتها للآن في العلاج
+
+46
+00:03:29,720 --> 00:03:33,480
+مباشرة؟ دولة على أنظر إلى ما يسمى ال selective
+
+47
+00:03:33,480 --> 00:03:37,300
+toxicity طبعا يمكن تكون زميسا من البكتيريا زميسا
+
+48
+00:03:37,300 --> 00:03:41,740
+وكلمة selective toxicity تعني it should be toxic
+
+49
+00:03:41,740 --> 00:03:44,500
+to the bacteria of the microorganism but not to
+
+50
+00:03:44,500 --> 00:03:49,500
+the host طبعا هذا ideally أنتوا بتسمعوا أنه معبر
+
+51
+00:03:49,500 --> 00:03:53,940
+ال antibiotics إلها بعض ال side effectsيعني
+
+52
+00:03:53,940 --> 00:03:57,820
+بنسالين ممكن تكون عندك hyper sensitivity بعض ال
+
+53
+00:03:57,820 --> 00:04:00,340
+antibiotic ممكن تعمل رارق بعض ال antibiotic ممكن
+
+54
+00:04:00,340 --> 00:04:07,940
+تعمل liver toxicity liver toxicity فالمصطلح هنا هو
+
+55
+00:04:07,940 --> 00:04:12,560
+على المستوى الخلوي أن يكون تأثيره على البكتيريا
+
+56
+00:04:12,560 --> 00:04:19,420
+وليس على خلالها العادى الطبق اللي قدامكوا هو الطبق
+
+57
+00:04:19,420 --> 00:04:24,790
+الشهير لالكزي .. لAlexander Flemingاللي كان مهمل
+
+58
+00:04:24,790 --> 00:04:28,850
+راح إجازة و سافر أطباق على الطاولة المخوضة هنا
+
+59
+00:04:28,850 --> 00:04:32,090
+إجرائية لازم نعقمهم قبل ما نروح على الإجازة يعني
+
+60
+00:04:32,090 --> 00:04:35,290
+يوم الأربع إذا عندنا الخميس و الجمعة إجازة لازم
+
+61
+00:04:35,290 --> 00:04:37,790
+نعقم كل أطباق ما نخليها موجودة في المطبق فدرك
+
+62
+00:04:37,790 --> 00:04:42,070
+الأطباق و راح إجازة و رجع لفت مقرره قبل ما يكتب
+
+63
+00:04:42,070 --> 00:04:46,730
+الأطباق أنه كان عنده طبق مليء بمستعمرات اللي
+
+64
+00:04:46,730 --> 00:04:49,630
+شايفينها هذه الـstaphylococcus aureus
+
+65
+00:04:52,850 --> 00:04:57,310
+ولا من فطر ولا من Penicillium وان المنطقة المحيطة
+
+66
+00:04:57,310 --> 00:05:00,690
+بالفطر بلغة المستعمرات تختفي تذوب ده فتلاحظ
+
+67
+00:05:00,690 --> 00:05:04,650
+المقارنة هنا وهنا يعني صغير جدا المستعمرات بواق
+
+68
+00:05:04,650 --> 00:05:09,010
+المستعمرات ومتأكد ان كان فيها مستعمرات كبيرة فكان
+
+69
+00:05:09,010 --> 00:05:12,050
+لابد ورا سنتاش منطقي لابد ان يكون هناك شيء في
+
+70
+00:05:12,050 --> 00:05:13,110
+الفطر قتله
+
+71
+00:05:17,420 --> 00:05:20,640
+طلعوا سنة الف وتسعمائة و كده؟ تمانية و عشرين،
+
+72
+00:05:20,640 --> 00:05:24,200
+اتطلعوا اول توظيف للبنسالين و ابتش كان؟ الف
+
+73
+00:05:24,200 --> 00:05:28,440
+وتسعمائة و أربعين، ده لما انا ابدا انتقال مركبات و
+
+74
+00:05:28,440 --> 00:05:31,400
+ادخلها في الخدمة العلاجية بيطلع بقطر، حتى في زمننا
+
+75
+00:05:31,400 --> 00:05:35,460
+هذا، زمن السرعة، بدنا من خمس الى خمساشر سنة حتى
+
+76
+00:05:35,460 --> 00:05:38,860
+نتكلم عن مركب ان ندخل في الخدمة العلاجية، لإن بمر
+
+77
+00:05:38,860 --> 00:05:45,350
+بمراحل testingطبعا هذا السيطرة على جشع الشركات
+
+78
+00:05:45,350 --> 00:05:49,810
+الأدوية اللي مقدت تزور في بعض الدراسات ففي مؤسسات
+
+79
+00:05:49,810 --> 00:05:52,850
+الآن زي ال FDA full of drugs administration تعمل
+
+80
+00:05:52,850 --> 00:05:55,730
+testing هي service لها لهادي الشركات تضمن إن هو
+
+81
+00:05:55,730 --> 00:06:00,010
+المركز بالفعل safe وحتى مع الضمان هذا للأسف كمان
+
+82
+00:06:00,010 --> 00:06:03,110
+لجينا بعض ال drugs اللي نزلت في السوق و بعدها تم
+
+83
+00:06:03,110 --> 00:06:06,310
+ايه ساعد على إنه بالفعل لجوا إليها side effect
+
+84
+00:06:06,310 --> 00:06:13,780
+مكان مرضي في 1940كل من هارد فلوري و إيرنست شين عمل
+
+85
+00:06:13,780 --> 00:06:17,320
+أول دراسات سيريرية و بعدها دخل الخدمة منذ اكتشاف
+
+86
+00:06:17,320 --> 00:06:21,240
+ال penicillin و تنظيفه بدأت الاكتشافات تبع ال
+
+87
+00:06:21,240 --> 00:06:24,900
+antibiotics تتوارى صار عننا يعني تخمة بال
+
+88
+00:06:24,900 --> 00:06:28,380
+antibiotics كتير عن ال antibiotics لدرجة اشعارنا
+
+89
+00:06:28,380 --> 00:06:33,220
+بالغرور انه علم موجود معانا لكل مايكروفات الدنيا
+
+90
+00:06:33,220 --> 00:06:33,940
+antibiotics
+
+91
+00:06:40,000 --> 00:06:43,440
+بتقول هذا باعطينا قائمة باسماء البكتيريا والفطريات
+
+92
+00:06:43,440 --> 00:06:47,820
+وال artenomycetes اللي بينتج منها أشهر أنواع ال
+
+93
+00:06:47,820 --> 00:06:51,900
+antibiotics مثلا ال bacillus satilis بينتج منها
+
+94
+00:06:51,900 --> 00:06:55,740
+البستراسين وال bacillus polymyxa بينتج منها
+
+95
+00:06:55,740 --> 00:06:59,260
+polymyxin B وهدول التنين Antibiotic بستراسين غير
+
+96
+00:06:59,260 --> 00:07:03,720
+مر معانا لما درسنا ال group A streptococci وميزنا
+
+97
+00:07:03,720 --> 00:07:06,420
+بينها بال Beta Hemolytic عملنا فحص البستراسين
+
+98
+00:07:13,370 --> 00:07:17,030
+بيوجين الكوكاية هو في البيوجين الكوكساية طيب
+
+99
+00:07:17,030 --> 00:07:21,790
+الأكتينو مايسيتس اللي قلتلكم مرة و لما كانت دي
+
+100
+00:07:21,790 --> 00:07:28,010
+نتيجتي أول مشيتها الأكتينو مايسيتس بالفعل they are
+
+101
+00:07:28,010 --> 00:07:30,110
+wonderful microbes يعني ميكروبات من الميكروبات
+
+102
+00:07:30,110 --> 00:07:36,140
+الرائعة جدا زمان أحكيلكم قصة زمانالعجايز ..
+
+103
+00:07:36,140 --> 00:07:38,080
+العجايز اللي هم كانوا صغار و بيولدوا يعني فيهم
+
+104
+00:07:38,080 --> 00:07:44,420
+بالأيام، لما ابنها كان يصير عنده أصماط، التهاب،
+
+105
+00:07:44,420 --> 00:07:46,740
+لما كان عنده ال border الترك زي اللي .. اللي
+
+106
+00:07:46,740 --> 00:07:51,460
+بيستخدموه اليوم، كانوا يجيبوا طينة حمرة جافة و
+
+107
+00:07:51,460 --> 00:07:53,380
+يطحنوها و ينخلوها عبر الشارع
+
+108
+00:08:04,750 --> 00:08:09,270
+فهر يعني لما يكون تراب جاف وغير مخلوق بمواد مؤذية
+
+109
+00:08:09,270 --> 00:08:16,990
+من مخلفات أدمية وغيرها وحيانية فهر طبعا الجزئين
+
+110
+00:08:19,250 --> 00:08:22,870
+كان تم لأن جزء من الطينة كان فيكون فيه antibiotics
+
+111
+00:08:22,870 --> 00:08:27,210
+لوجود ال actinomycetes في هذه البيئة فال
+
+112
+00:08:27,210 --> 00:08:30,730
+actinomycetes و يبتنمو بتنتج antibiotics و لإن
+
+113
+00:08:30,730 --> 00:08:35,230
+الغبار صار particles ضعيفة صغيرة جدا فمنعت من
+
+114
+00:08:35,230 --> 00:08:38,850
+اهتكاك الجلب للجد فقلبت من ايه؟ من التهيير جبتالي
+
+115
+00:08:38,850 --> 00:08:43,250
+ممكن كان سهر الشفاء إذا ال antibioticsقديما
+
+116
+00:08:43,250 --> 00:08:48,830
+معروفة، لكن المصريين علجوا .. القدراء علجوا مرضاهم
+
+117
+00:08:48,830 --> 00:08:52,350
+بالخبز المعفن، تمام؟ كانوا ياخدوا من الخبز .. و
+
+118
+00:08:52,350 --> 00:08:55,530
+الخبز المعفن ايش هو؟ بنسيمين، تمام؟ بنسيمين،
+
+119
+00:08:55,530 --> 00:09:00,910
+وبالتالي كان البشر قدرقوا في بعض الأحيان بفطرتهم،
+
+120
+00:09:00,910 --> 00:09:04,890
+بملاحظاتهم، لذا كانوا بيقولوا ان ال science is a
+
+121
+00:09:04,890 --> 00:09:06,210
+field of observation
+
+122
+00:09:09,680 --> 00:09:12,900
+الدربيشن هي كتير مهمة وفرق بين الناس .. انت
+
+123
+00:09:12,900 --> 00:09:20,280
+بتلاحظوا شغل ان معظم المكتشفين والولماء يلهم نظرة
+
+124
+00:09:20,280 --> 00:09:26,780
+مختلفة عن الآخرين في ال .. في الظواهر، الظواهر كل
+
+125
+00:09:26,780 --> 00:09:29,360
+بيشاهدها وكل بيشوفها لأن بيجي عالم
+
+126
+00:09:36,020 --> 00:09:41,140
+الحق المتمرس غالبا بيسأل الأسئلة اللى بتأدي إلى
+
+127
+00:09:41,140 --> 00:09:46,120
+نتائج من ضمن هذه الاكتشافات نرجع للأكتينوميسات
+
+128
+00:09:46,120 --> 00:09:50,000
+الرائع اللى قول عنها رائع streptomyosis nodosis
+
+129
+00:09:50,000 --> 00:09:53,360
+streptomyosis venezuela streptomyosis professions
+
+130
+00:09:56,160 --> 00:09:59,540
+نتعود عليهم Steratomyces nodosis هدولة مش ممرضات
+
+131
+00:09:59,540 --> 00:10:03,260
+هدولة منتجات هدولة يعني بكتيريا صديقة بكتيريا
+
+132
+00:10:03,260 --> 00:10:07,900
+صديقة Steratomyces Venezuela تنتج كلرمفينيكول خدوا
+
+133
+00:10:07,900 --> 00:10:11,340
+بالكم من كل القائمة رقم تلاتة اللي هو ال
+
+134
+00:10:11,340 --> 00:10:17,400
+Amphotericin B شايفينه؟ ورقم اتنين من تحت اللي هو
+
+135
+00:10:17,400 --> 00:10:22,040
+Glycepholvainأمفوترسين بي و graceful بي مانوا
+
+136
+00:10:22,040 --> 00:10:27,680
+بدولة هدولة التانين antifungal يعني بيقتلوا الفطر
+
+137
+00:10:27,680 --> 00:10:36,380
+يعني والباقي antibacterials يعني تقتل البركيلة
+
+138
+00:10:36,380 --> 00:10:40,080
+راحظوا إيش سمت ال .. ال .. ال .. ال .. ال anti تبع
+
+139
+00:10:40,080 --> 00:10:43,520
+الفطر ياسا منها antifungal تغيب ال antibiotic
+
+140
+00:10:43,520 --> 00:10:44,580
+مقصورة
+
+141
+00:10:52,500 --> 00:10:55,600
+Kills microorganisms صح؟ لكن هي واقع الأمر
+
+142
+00:10:55,600 --> 00:11:00,620
+الاستخدام الحقيقي لها غالب من ضد البكتيريا لأنه لو
+
+143
+00:11:00,620 --> 00:11:05,140
+قلنا الفانجل هو microorganism صح؟ لكن لما بنطلق
+
+144
+00:11:05,140 --> 00:11:09,420
+مركب ضدهش بنقول انتي فانجل لبروتزوفا بنقول انتي
+
+145
+00:11:09,420 --> 00:11:13,520
+بروتزوفا لدراج طبعا؟ اقولكوا حتى لميكوبكتيريا اللي
+
+146
+00:11:13,520 --> 00:11:17,320
+هي بكتيريا لما بنعطيها انتي بنقول انتي ميكوبكتيريا
+
+147
+00:11:17,320 --> 00:11:20,240
+علشان نميزها فغالب ال antibiotics هي ال common
+
+148
+00:11:23,700 --> 00:11:27,420
+Stryptomyces nodosis Amphotericin B Stryptomyces
+
+149
+00:11:27,420 --> 00:11:32,620
+Venezuella Cloramfenicol Stryptomyces Erythracines
+
+150
+00:11:32,620 --> 00:11:40,500
+Stryptomyces Erythrias Erythromycin Stryptomyces
+
+151
+00:11:40,500 --> 00:11:44,700
+Ephradiei Neomycin Stryptomyces Grecius
+
+152
+00:11:44,700 --> 00:11:46,040
+Stryptomycin
+
+153
+00:11:49,980 --> 00:11:52,660
+لأ طلعوا ليه على التلتة اللي احنا ذاكرتهم اخر اشي
+
+154
+00:11:52,660 --> 00:11:56,320
+نيوميسين و Striptomycin و Gendamycin هدول التلتة
+
+155
+00:11:56,320 --> 00:12:00,580
+عائلة واحدة ك Antibiotics العائلة دلوقت هم اسمها
+
+156
+00:12:00,580 --> 00:12:06,020
+Aminoglycosides Aminoglycosides لكن لفظ المنتجات
+
+157
+00:12:06,020 --> 00:12:08,840
+من وين؟ هل من نفس العائلة؟ هل لقينا micro
+
+158
+00:12:08,840 --> 00:12:14,020
+monospora و لقينا اللي هو Striptomyces يبقى ..
+
+159
+00:12:14,020 --> 00:12:19,700
+بنقدر نقول انهالتصنيف تبع ال Antibiotics could be
+
+160
+00:12:19,700 --> 00:12:23,380
+chemical classification, aminoglycosides could be
+
+161
+00:12:23,380 --> 00:12:28,520
+ال origin تبعه من بكتيريا و لا من فطريات مثلا هل
+
+162
+00:12:28,520 --> 00:12:31,440
+هو باسع الطيف و لا ضيق الطيف؟ بكتيريا سايدر و لا
+
+163
+00:12:31,440 --> 00:12:34,960
+بكتيريا هو؟ static، لأن في تصنيفات متنوعة و
+
+164
+00:12:34,960 --> 00:12:37,820
+متعددة، همر عليهم واحد واحد إن شاء الله
+
+165
+00:12:41,110 --> 00:12:45,210
+خلاصة مايكرو موانس بورا تاكورا فنجاي سفارس بوريوم
+
+166
+00:12:45,210 --> 00:12:49,690
+بنسليوم جريسيو فولفام و بنسليوم نوتاتم طلع بنسليوم
+
+167
+00:12:49,690 --> 00:12:53,350
+جريسيو فولفام طلع جريسيو فولفام بيه اللي هو Anti
+
+168
+00:12:53,350 --> 00:12:57,390
+-Fungal بينما بنسليوم نوتاتم طلع اللي هو ال
+
+169
+00:12:57,390 --> 00:13:01,490
+بنسيلين اللي هو Anti-Bacterial لذلك مافيش قاعدة
+
+170
+00:13:01,490 --> 00:13:06,700
+تحكم مين بنتج مينantibacteria مرة بكتيريا تنتج
+
+171
+00:13:06,700 --> 00:13:10,580
+antifungal واحدة طبعا لإن هذا أصلا كلمة antibiotic
+
+172
+00:13:10,580 --> 00:13:12,720
+من هنا أنا نسيت أحكي من هنا كلمة antibiotic إجت
+
+173
+00:13:12,720 --> 00:13:18,140
+antibiotic قدت من ظاهر اسمها Antibiosis بيوسس حياة
+
+174
+00:13:18,140 --> 00:13:23,200
+Anti ضد الحياة فكان في ظاهر اللي هي ظاهرة إنه
+
+175
+00:13:23,200 --> 00:13:29,680
+البكتيريا في في التربة لا توجد بشكل منفرد وإنها
+
+176
+00:13:29,680 --> 00:13:33,170
+توجد بشكل communitiesفاكرين لما قولنا competition
+
+177
+00:13:33,170 --> 00:13:37,690
+مرة مصطلح كتير مهم و منافسة ان وجود بكتيريا بتنافس
+
+178
+00:13:37,690 --> 00:13:41,810
+بتحدي طب كيف البكتيريا بتنافس؟ بعض على البعض أحد
+
+179
+00:13:41,810 --> 00:13:45,890
+أدايات المنافسة is to produce compounds that kills
+
+180
+00:13:45,890 --> 00:13:49,750
+others غير ان ناخد الحديد مصبوح فكرا الحديد او
+
+181
+00:13:49,750 --> 00:13:53,410
+نتنافس على نوترات معينة ممكن كمان ننتج ملكمات لقطع
+
+182
+00:13:59,190 --> 00:14:04,570
+طلعولي على الاطار اللي باللون الأزرق اللي بيقول
+
+183
+00:14:04,570 --> 00:14:07,670
+micro bacteria, ground negative bacteria, ground
+
+184
+00:14:07,670 --> 00:14:10,990
+positive bacteria, chlamydia, fungi, protozoa,
+
+185
+00:14:11,050 --> 00:14:14,410
+helminthes and viruses هذه هي ال micro organisms
+
+186
+00:14:14,410 --> 00:14:17,490
+ولا في شيء جديد أنكوا، هي ال micro organisms اللي
+
+187
+00:14:17,490 --> 00:14:20,990
+بيسميها micro organisms طلعولي على ال tetracycline
+
+188
+00:14:21,870 --> 00:14:24,730
+تترا ساكلين تأثيره على مين؟ على الـ ground
+
+189
+00:14:24,730 --> 00:14:27,150
+negative، على الـ ground positive، وعلى الـ
+
+190
+00:14:27,150 --> 00:14:31,470
+chlamydia كان مدرسنا الله يرحمه أكيد توفي كان
+
+191
+00:14:31,470 --> 00:14:35,130
+بيقول إذا ذهبت بالامتحان على بكتيريا و Antibiotic
+
+192
+00:14:35,130 --> 00:14:40,130
+من ال drug of choice حطه تترا ساكلين بنسبة 80%
+
+193
+00:14:40,130 --> 00:14:43,690
+هيكون الجوابق صحيح لأن لو اللحظة ال range بتاعه
+
+194
+00:14:46,930 --> 00:14:51,170
+البيانات زي الفنسالين it is وعادة في نرو نرو رانج
+
+195
+00:14:51,170 --> 00:14:55,870
+بس على الإجراءات مستر بكتيريا بينما ال .. ال .. ال
+
+196
+00:14:55,870 --> 00:14:58,510
+.. الستربتوميسين أثر على الميكو بكتيريا و أثر على
+
+197
+00:14:58,510 --> 00:15:02,050
+الإجراءات نيجاتية هناك ال ISO ميازات فقط على
+
+198
+00:15:02,050 --> 00:15:05,950
+الميكو بكتيريا إذا نقدر نقسم البكتيريا إلى قسمين
+
+199
+00:15:05,950 --> 00:15:11,130
+رئيسيا واحد اللي هو broad spectrum ويسعى الطريف و
+
+200
+00:15:11,130 --> 00:15:18,180
+narrow spectrum والسؤال اللي لازم أسأله مين أحسن؟و
+
+201
+00:15:18,180 --> 00:15:23,980
+نفس الغلطات اللي من نسخه المرضى، أنا قلت الجواب من
+
+202
+00:15:23,980 --> 00:15:25,500
+مبارزنا، الجواب دي من مبارزنا
+
+203
+00:15:29,950 --> 00:15:34,450
+بفضل بفضل ال neuro spectrum لما أكون عارف لما أكون
+
+204
+00:15:34,450 --> 00:15:37,910
+عارف بفضل ال neuro spectrum لما أكون مش عارف و
+
+205
+00:15:37,910 --> 00:15:42,550
+الحالة خطيرة مابتستناش من injustice مثلا نستخدم ال
+
+206
+00:15:42,550 --> 00:15:45,590
+broad spectrum إذا it really depends ورمه هذا
+
+207
+00:15:45,590 --> 00:15:50,750
+موجود و هذا موجود و يجب علينا أن نعلم متى نوظف كل
+
+208
+00:15:50,750 --> 00:15:55,110
+واحدة بالضبط نفس السؤال هنسأله bactericidal ولا
+
+209
+00:15:55,110 --> 00:15:57,250
+bacterostatic مين أحسن؟
+
+210
+00:16:01,790 --> 00:16:05,330
+إذا كان في الدم فورمينت سبتسيميا، يعني سبتسيميا
+
+211
+00:16:05,330 --> 00:16:08,370
+بشكل كبير، لأ نفضّل الـbacteriostatic لأن الصحيح
+
+212
+00:16:08,370 --> 00:16:11,150
+ده هيقدّي إلى الـGram Negative Septic Shock اللي
+
+213
+00:16:11,150 --> 00:16:15,730
+حكينا عنه، يبقى بدنا نعرف أنه Broad Spectrum و
+
+214
+00:16:15,730 --> 00:16:18,310
+Narrow Spectrum، على أي أساس صدفناهم Broad و
+
+215
+00:16:18,310 --> 00:16:24,230
+Narrow؟ على هل التأثير واسع، المجال و اللي هتضير،
+
+216
+00:16:24,230 --> 00:16:24,710
+المجال
+
+217
+00:16:27,460 --> 00:16:30,440
+طبعا كلمة broad spectrum ايش بيقبلها قولنا؟ now ال
+
+218
+00:16:30,440 --> 00:16:32,660
+spectrum هي التعريف الموجود في مصطلح super
+
+219
+00:16:32,660 --> 00:16:37,300
+infection حقيقة التعريفه شويه يعني confusing بس
+
+220
+00:16:37,300 --> 00:16:39,660
+خلينا نوضحه ما تطلعوا على السبوك و لا تطلعوا على
+
+221
+00:16:39,660 --> 00:16:44,260
+الكلام اللي بنحكي لأن احنا بصير انها infection
+
+222
+00:16:44,260 --> 00:16:49,320
+طبيعي انه ناخد antibioticالان لو البكتيريا اللى
+
+223
+00:16:49,320 --> 00:16:52,620
+عملت ال infection الاولى استجابت ال antibiotics،
+
+224
+00:16:52,620 --> 00:16:57,660
+تمام؟ ال antibiotic هل بيقتل فقط البكتيريا المسببة
+
+225
+00:16:57,660 --> 00:17:01,000
+بال infection ولا بيقتل معاها مايكروبات اخرى؟ اللى
+
+226
+00:17:01,000 --> 00:17:04,620
+بيقتل اى مايكروب هو عنده قدرة على قتله، مالميزش
+
+227
+00:17:04,620 --> 00:17:07,860
+بين infection و مش infection، اى مايكروب موجود
+
+228
+00:17:07,860 --> 00:17:13,730
+بيحتك فيه بيقتله و بيقدر يقتل يقتلهيبقى اتوقع لو
+
+229
+00:17:13,730 --> 00:17:17,390
+انا باخد عندي infection ان عندي اتهاب في ال oral
+
+230
+00:17:17,390 --> 00:17:21,710
+cavity في ال led ده مثلا واخدت antibiotic الفن فيه
+
+231
+00:17:21,710 --> 00:17:27,370
+normal flora مات البكتيرا و وراق خفت ال led اتهاب
+
+232
+00:17:27,370 --> 00:17:31,730
+ال led تلالي اشي تاني زي و كان ده البكانس الفن صار
+
+233
+00:17:31,730 --> 00:17:39,110
+كله أبيض و حم و مزعج جدا و مؤلممش اللي سببه؟ ان
+
+234
+00:17:39,110 --> 00:17:42,290
+انا خدت antibiotic ل infection فصار في infection
+
+235
+00:17:42,290 --> 00:17:46,950
+تانية فهي بيسموها super infection بس واضح انه
+
+236
+00:17:46,950 --> 00:17:51,270
+عجبنا التعريف احنا تمام؟ ان infection هو infection
+
+237
+00:17:51,270 --> 00:17:54,070
+ناتج على ال blood blood فهي في الواقع الأولى هي ال
+
+238
+00:17:54,070 --> 00:17:58,150
+infection ناتج عن مين؟ عن استخدام ال antibiotic
+
+239
+00:17:58,150 --> 00:17:59,750
+غالبا احنا استخدام ال antibiotic عشان انا عايز
+
+240
+00:17:59,750 --> 00:18:00,850
+اصلا infection
+
+241
+00:18:11,690 --> 00:18:15,110
+الشيء الوحيد اللي اختلف ان احنا دخلنا انتياباتيك
+
+242
+00:18:15,110 --> 00:18:18,010
+قتل ال normal flora ففعب ال normal flora او
+
+243
+00:18:18,010 --> 00:18:21,010
+مايكروفات اخرى استطاعت في غياب ال normal flora
+
+244
+00:18:21,010 --> 00:18:25,390
+انها تعمل growth و تعمل infection مرة تانية اشهر
+
+245
+00:18:25,390 --> 00:18:29,390
+مثال عليها الادم النموذج ال clostridium difficile
+
+246
+00:18:29,390 --> 00:18:33,110
+normal flora of ventricular tract
+
+247
+00:18:39,130 --> 00:18:41,570
+وإذا عادة محدودة وماوصلش لـ Quorum Sensing، مش
+
+248
+00:18:41,570 --> 00:18:45,430
+هتمدج تكسين سبعتها، لو أخدنا antibiotic for
+
+249
+00:18:45,430 --> 00:18:48,190
+prolonged period أو بعض أنواع من ال antibiotic حتى
+
+250
+00:18:48,190 --> 00:18:50,670
+for short period، بصير فيه خلل في الموضوع في
+
+251
+00:18:50,670 --> 00:18:54,250
+الورقة، فبصير لها overgrowth، إذا صارت overgrowth،
+
+252
+00:18:54,250 --> 00:18:59,000
+يبقى صار فانديش الآنProduction لتكسين A وB تبعونها
+
+253
+00:18:59,000 --> 00:19:03,360
+وبتعمل condition سيء ب resistance ديارية، ديارية
+
+254
+00:19:03,360 --> 00:19:06,840
+بتتطور لفترة طويلة و condition بيسموه Antibiotic
+
+255
+00:19:06,840 --> 00:19:10,340
+-induced pseudomembraneous colitis ده نبع على أنه
+
+256
+00:19:10,340 --> 00:19:14,160
+الـ colitis هذه ناجم عن استخدام الـ Antibiotic
+
+257
+00:19:14,160 --> 00:19:17,580
+كلمة Antibiotic-induced يعني اللي سبب إنتاجها هو
+
+258
+00:19:17,580 --> 00:19:22,910
+الـ Antibiotic مصطلحين أخرين في اللوحةعندي بكتيري
+
+259
+00:19:22,910 --> 00:19:26,930
+سايدل وبكتيري بكتيري أستاتيك كوننا كالوس بكتيريا
+
+260
+00:19:26,930 --> 00:19:30,090
+وإنهم استجرارات البكتيريا إيش الفرق من اتنين من
+
+261
+00:19:30,090 --> 00:19:36,250
+ناحية عملية؟ من ناحية عملية إنه لو أزلنا البكتيري
+
+262
+00:19:36,250 --> 00:19:41,630
+سايدل بعد فترة من الزمن البكتيريا لن تعود للانقسام
+
+263
+00:19:41,630 --> 00:19:45,370
+بينما البكتيري أستاتيك لو أزلنا البكتيري أستاتيك
+
+264
+00:19:45,370 --> 00:19:48,970
+هذا بعد فترة بصير البكتيري اتن من أحسن
+
+265
+00:19:53,570 --> 00:19:58,190
+البكتيريو أستاتيك البكتيريو أستاتيك له يعني روناق،
+
+266
+00:19:58,190 --> 00:20:00,630
+إيش هو روناق طبعا؟ بتعرف .. بتعرف ال Vibrio
+
+267
+00:20:00,630 --> 00:20:03,670
+cholera اللي أشرحناها قبل المحاضرة التانية؟ ال
+
+268
+00:20:03,670 --> 00:20:07,790
+Vibrio cholera على خطورتها وعلى يعني بالعادية على
+
+269
+00:20:07,790 --> 00:20:11,670
+التترا سايكلين، التترا سايكلين، التترا سايكلين
+
+270
+00:20:11,670 --> 00:20:17,530
+وصفه بكتيري أستاتيك، بكتيريو أستاتيك، ومع ذلك very
+
+271
+00:20:17,530 --> 00:20:19,110
+effective، بتعرف الطعم؟
+
+272
+00:20:23,450 --> 00:20:28,010
+إلا إنه خطير المرض إلا إنه bacteriostatic وما
+
+273
+00:20:28,010 --> 00:20:31,750
+ننساش إنه فيه جهات اسمه جهاز مانعة احنا بنوقف نشاط
+
+274
+00:20:31,750 --> 00:20:34,830
+البكتيريا و بنخل الجهاز المانعة برضه يقوم بالدور
+
+275
+00:20:34,830 --> 00:20:38,510
+المانوت هذا
+
+276
+00:20:38,510 --> 00:20:43,270
+كارتون، كارتون حلو كتير، بوضح إن على الجهة دي
+
+277
+00:20:43,270 --> 00:20:46,570
+bacteriostatic ماخدهم أسرة مربطة، مظبوطة
+
+278
+00:20:46,570 --> 00:20:51,210
+البكتيريا، بينما البكتيري سهل مش عاملمافيش أسرة
+
+279
+00:20:51,210 --> 00:20:54,550
+خلاص هيبقى قاتلة على طول طبعا في أمثلة هنا زي ال
+
+280
+00:20:54,550 --> 00:20:57,430
+chloramphenicol الارتروميسين والكليمناميسين ال
+
+281
+00:20:57,430 --> 00:21:00,870
+cellophanomyces ترانميثوبريم وتترا ساكلينز هدولة
+
+282
+00:21:00,870 --> 00:21:04,010
+اللي بياخدوا أسرة اللي هم بكتيريه أستاتيك ع الجهة
+
+283
+00:21:04,010 --> 00:21:08,630
+المقابلة فينديل أمينوجليكوسايدس و ال bedalactam
+
+284
+00:21:08,630 --> 00:21:11,230
+-antibiotics و ال vancomycin اللي كونلونز هدولة
+
+285
+00:21:11,230 --> 00:21:15,490
+بيعملوا بكتيريه صحيح بقى هنذكر جملة هنا لازم ننتبه
+
+286
+00:21:15,490 --> 00:21:20,270
+إليها كويسكلمة bactericide أو bacteriostatic is a
+
+287
+00:21:20,270 --> 00:21:24,650
+matter of concentration يعني لو زدنا ال
+
+288
+00:21:24,650 --> 00:21:29,910
+bacteriostatic concentration ممكن تقدر ديها القتل
+
+289
+00:21:29,910 --> 00:21:34,610
+لكن ليش احنا وصلنا بعض ال antibiotic بإنها
+
+290
+00:21:34,610 --> 00:21:38,070
+bacteriostatic at the therapeutic dose الجرعه
+
+291
+00:21:38,070 --> 00:21:41,970
+علاجية اللي استخدمت في العلاج هي bacteriostatic
+
+292
+00:21:41,970 --> 00:21:46,370
+كلمة جرعه دوس concentration صح او لا؟و العكس كمان
+
+293
+00:21:46,370 --> 00:21:49,310
+بالنسبة لمن؟ لجهة .. لجهة .. ل .. ال .. ال .. ال
+
+294
+00:21:49,310 --> 00:21:52,550
+bactericide اللي كانت بالفعل هو bactericide عادية
+
+295
+00:21:52,550 --> 00:21:55,870
+تانية اتنين بتباهوا مع بعض و حقها لهم ان انا
+
+296
+00:21:55,870 --> 00:21:59,190
+اتباههم لو اقوله انا انديه كلهم من نوع واحد اللي
+
+297
+00:21:59,190 --> 00:22:02,250
+بقتلهم gram negative زي ال .. ال .. ال .. ال .. ال
+
+298
+00:22:02,250 --> 00:22:03,410
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+299
+00:22:03,410 --> 00:22:04,710
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+300
+00:22:04,710 --> 00:22:04,910
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+301
+00:22:04,910 --> 00:22:06,850
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+302
+00:22:06,850 --> 00:22:07,010
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+303
+00:22:07,010 --> 00:22:07,430
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+304
+00:22:07,430 --> 00:22:12,070
+.. ال .. ال .. ال .. ال .. ال .. ال ..
+
+305
+00:22:15,180 --> 00:22:18,220
+اللوحة الأهم على الإطلاق لانجب ابدأ فيها بدي ارجع
+
+306
+00:22:18,220 --> 00:22:23,420
+برجوع اتكلم عن ال antibiotics او ال antimicrobials
+
+307
+00:22:23,420 --> 00:22:26,180
+انا قولت قبل شوية انه penicillins وcephalosporins
+
+308
+00:22:26,180 --> 00:22:28,940
+و amino glycosides اسماء هذي الأسماء اللي هون
+
+309
+00:22:28,940 --> 00:22:32,840
+جيبناها هذي chemical classification يعني مش احنا
+
+310
+00:22:32,840 --> 00:22:36,000
+اللي سميناها الكيميائيين بس ال microbiologists هم
+
+311
+00:22:36,000 --> 00:22:38,840
+اللي سموا جهود ال spectrum غيره spectrum أما
+
+312
+00:22:38,840 --> 00:22:42,380
+الكيميائين هم اللي عطوا الأسماء للمراكبات لإنه كل
+
+313
+00:22:42,380 --> 00:22:47,750
+مراكب له صفات محددةإذا تصنيف المراكبات كيميائيا مش
+
+314
+00:22:47,750 --> 00:22:50,570
+منانا تصنيفهم بكتيري صادق من بكتيري صادق منانا
+
+315
+00:22:50,570 --> 00:22:53,150
+تصنيفهم broad spectral و narrow spectral منانا
+
+316
+00:22:53,150 --> 00:22:56,450
+التصنيف الأهم على الإطلاق اللي قدامه على السبورة
+
+317
+00:22:56,450 --> 00:23:02,350
+هو based on the site affected أو targeted يعني
+
+318
+00:23:02,350 --> 00:23:05,370
+عالميا بيشتغل ال .. ال .. ال antibiotic على ال
+
+319
+00:23:05,370 --> 00:23:09,210
+cell wall و لا بيشتغل على ال cell membrane و لا
+
+320
+00:23:09,210 --> 00:23:13,660
+على ال protein synthesisأو كـ Antimetabolites كم
+
+321
+00:23:13,660 --> 00:23:17,800
+كده اذا ذكرتها انا؟ خمسة ندوا واحد منهم بالترتيب
+
+322
+00:23:17,800 --> 00:23:22,260
+من بره جوا cell wall cell membrane خلينا نقول
+
+323
+00:23:22,260 --> 00:23:25,280
+protein synthesis نكليك أسد synthesis وفي
+
+324
+00:23:25,280 --> 00:23:30,560
+antimetabolites antimetabolites يعني it inhibits a
+
+325
+00:23:30,560 --> 00:23:34,480
+certain metabolic step في الخلية عدى الأربع اللي
+
+326
+00:23:34,480 --> 00:23:41,850
+احنا ذكرناهم الأخرين تعالوا نطلع على ال cellCell
+
+327
+00:23:41,850 --> 00:23:46,170
+wall inhibitions of cell wall synthesis الانبجي
+
+328
+00:23:46,170 --> 00:23:49,470
+معناه اللي حاكى اي antibiotic او antimicrobial
+
+329
+00:23:49,470 --> 00:23:52,210
+اللي هو علاقة بال cell wall inhibition بصنفه تحت
+
+330
+00:23:52,210 --> 00:23:54,870
+مين cell wall inhibitors cell membrane inhibitors
+
+331
+00:23:54,870 --> 00:23:57,830
+protein synthesis inhibitors صلاة تصنيف جديد صلاة
+
+332
+00:23:57,830 --> 00:24:01,510
+تصنيف جديد و غالبا هو اللي احنا ليش التصنيف هذا
+
+333
+00:24:01,510 --> 00:24:05,230
+بهمنا لأن هو اللي بوضّحلك ال mechanism of action
+
+334
+00:24:05,230 --> 00:24:10,440
+تبع المركباتالـ Cephalosporins و الـ Penicillins
+
+335
+00:24:10,440 --> 00:24:13,880
+هم الانهيبترات يبقى ال chemical classification ايه
+
+336
+00:24:13,880 --> 00:24:17,420
+جبناها و حطيناها تحت تصنيع السل اللي هو ال
+
+337
+00:24:17,420 --> 00:24:23,240
+vancomycin-cadaric العمل تحت هنا injury to plasma
+
+338
+00:24:23,240 --> 00:24:25,400
+membrane اللي هو السل من برا طبعا السل من برا من
+
+339
+00:24:25,400 --> 00:24:29,460
+برا هو نفس السل من برا زي بوليميكسين B و ال
+
+340
+00:24:29,460 --> 00:24:34,640
+protein synthesis الجهة دي هما دولة ال protein
+
+341
+00:24:34,640 --> 00:24:37,990
+synthesisفي اندي فاكرين ال ال ال ال ال ال ال ال ال
+
+342
+00:24:37,990 --> 00:24:38,390
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+343
+00:24:38,390 --> 00:24:39,930
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+344
+00:24:39,930 --> 00:24:40,770
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+345
+00:24:40,770 --> 00:24:48,670
+ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+346
+00:24:48,670 --> 00:24:48,670
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+347
+00:24:48,670 --> 00:24:51,730
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+348
+00:24:51,730 --> 00:24:53,430
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+349
+00:24:53,430 --> 00:24:53,430
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+350
+00:24:53,430 --> 00:24:53,430
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+351
+00:24:53,430 --> 00:24:53,430
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+352
+00:24:53,430 --> 00:24:53,450
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+353
+00:24:53,450 --> 00:24:53,670
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+354
+00:24:53,670 --> 00:24:53,670
+ال ال ال ال ال ال ال ال
+
+355
+00:25:06,700 --> 00:25:09,900
+أتوقع منكوا تحضروا تحضروا نوكليك أسد سينتسيز
+
+356
+00:25:09,900 --> 00:25:12,040
+نوكليك أسد نوكليك أسد نوكليك أسد نوكليك أسد نوكليك
+
+357
+00:25:12,040 --> 00:25:12,660
+أسد نوكليك أسد نوكليك أسد نوكليك أسد نوكليك أسد
+
+358
+00:25:12,660 --> 00:25:13,320
+نوكليك أسد نوكليك أسد نوكليك أسد نوكليك أسد نوكليك
+
+359
+00:25:13,320 --> 00:25:13,940
+أسد نوكليك أسد نوكليك أسد نوكليك أسد نوكليك أسد
+
+360
+00:25:13,940 --> 00:25:22,640
+نوكليك أسد نوكليك أسد نوكليك أسد نوكليك أسد
+
+361
+00:25:22,950 --> 00:25:27,210
+ماتحفظهم مش، بس هفرهم و خلاص، لأ؟ ليش؟ لأن بدون
+
+362
+00:25:27,210 --> 00:25:32,010
+معرفة ال process نفسها صعب عليا أن اوصلكم فكرة كيف
+
+363
+00:25:32,010 --> 00:25:34,650
+كل step أو كل antibody لأن أنا قلت protein
+
+364
+00:25:34,650 --> 00:25:37,910
+synthesis ما أنا حاول اقوله على طول، دخل في
+
+365
+00:25:37,910 --> 00:25:41,510
+أدمغاتنا إنهم كلهم بيشتغلوا بنفس الطريقة، لأ، هي
+
+366
+00:25:41,510 --> 00:25:46,170
+المحصلة النهائية إن ال protein synthesis وقف، لكن
+
+367
+00:25:46,170 --> 00:25:49,640
+كل واحد مناللي انا بكرته فدولة بيشتغل بطريقة
+
+368
+00:25:49,640 --> 00:25:52,780
+مختلفة تانية و لازم نكون عارفين كل واحدة منهم مش
+
+369
+00:25:52,780 --> 00:25:56,700
+هنقدر نفهم الكلمة ماذا إلا إذا استرجعنا ل protein
+
+370
+00:25:56,700 --> 00:26:00,140
+synthesis أو ال cell wall synthesis دي
+
+371
+00:26:11,430 --> 00:26:16,550
+أعتقد أن ال mRNA اللي موجود تحت هذا على ال .. زي
+
+372
+00:26:16,550 --> 00:26:21,150
+ما هقشر الآن بالماوس هذا ال RNA إذا بتلاحظوا في
+
+373
+00:26:21,150 --> 00:26:25,690
+عندي chloramphenicol, erythromycin, tetracycline و
+
+374
+00:26:25,690 --> 00:26:27,110
+streptomycin
+
+375
+00:26:30,650 --> 00:26:34,070
+بتعرف تماما عن بعضها البعض كيميائيا يعني ال
+
+376
+00:26:34,070 --> 00:26:39,210
+stripomycin بيمثل عائلة اسمها aminoglycosides فيها
+
+377
+00:26:39,210 --> 00:26:42,430
+أكتر من عشرين antibiotics كلهم بيشتغلوا بالنفس
+
+378
+00:26:42,430 --> 00:26:44,870
+الطريقة تبع ال stripomycin aminoglycosides
+
+379
+00:26:44,870 --> 00:26:48,790
+بيشتغلوا على مين؟ على ال 30 أسرعي بوزوه مرساليونية
+
+380
+00:26:48,790 --> 00:26:53,010
+بيعملوا bulging bulging يعني نوع من النتوء لما
+
+381
+00:26:53,010 --> 00:26:55,890
+بيجي الإمارة ان هي بدل من يكون مستوي بيصير فارق
+
+382
+00:26:56,180 --> 00:27:00,300
+بيفرض ال nucleotides طب ال anticonon كيف بترتبط
+
+383
+00:27:00,300 --> 00:27:05,480
+فيه؟ لحظة ال anticonon مصمم انه يرتبط ب
+
+384
+00:27:05,480 --> 00:27:10,220
+confirmation محددة،الان ال confirmation اتغيرت مش
+
+385
+00:27:10,220 --> 00:27:14,060
+هقدر انا اترتبط وكل اشي في ال antibiotic very
+
+386
+00:27:14,060 --> 00:27:17,840
+specific عشان هيك قلتلكوا البكتيريا قادرة على
+
+387
+00:27:17,840 --> 00:27:22,800
+تكوين resistance بسهولة لل antibiotic لان it is
+
+388
+00:27:22,800 --> 00:27:24,000
+targeting
+
+389
+00:27:27,130 --> 00:27:33,110
+يعني مثلا البكتيريا غيرت .. هو اين برتبط ال .. ال
+
+390
+00:27:33,110 --> 00:27:36,070
+.. ال .. الستراتومايسين برتبط في لزوغ معين في ال
+
+391
+00:27:36,070 --> 00:27:40,210
+30S لو صار في modification بسيط في هذا اللزوغ
+
+392
+00:27:40,210 --> 00:27:43,330
+المستقبل للاستراتومايسين بيبطل الاستراتومايسين
+
+393
+00:27:43,330 --> 00:27:45,910
+يرتبط في الريبوزام وبالتالي بصير البكتيريا
+
+394
+00:27:45,910 --> 00:27:50,350
+resistant يعني يبقى اي change طفيف
+
+395
+00:27:54,400 --> 00:27:58,380
+أكرة الباب، قلنا بنفتح الباب من الأكرة، من إيد
+
+396
+00:27:58,380 --> 00:28:03,360
+الباب، الآن هي ال target تبعنا للولوج إلى الغرف أو
+
+397
+00:28:03,360 --> 00:28:06,940
+للخروج، بمعنى إنه هي ال target تبعنا، زي ما ال
+
+398
+00:28:06,940 --> 00:28:11,560
+antibiotic له target، بلّت أكرة، بس بلّت المسافر
+
+399
+00:28:11,560 --> 00:28:14,480
+اللي ما كانت بينها و بين الباب، 2، 3، 19، حين حطنا
+
+400
+00:28:14,480 --> 00:28:18,540
+إيدينا فيها، لزجناها بالباب، بقى إلا أكرة، و بتقول
+
+401
+00:28:18,540 --> 00:28:21,020
+ما نفس الوضع، فتفتح و هتخلق الباب، لكن أنا مش هقدر
+
+402
+00:28:21,020 --> 00:28:24,380
+أحط إيدي و أمسك و أقنع الأكرةيبقى لما يتغير ال
+
+403
+00:28:24,380 --> 00:28:28,160
+target كمان لما يتغير ال target ال antibiotic
+
+404
+00:28:28,160 --> 00:28:32,380
+بيفشل في انه يقوم بوظيفته من هنا تبدأ عمالة اللي
+
+405
+00:28:32,380 --> 00:28:35,040
+هو ال resistance طبعا ال bacteria زي ما تقولي
+
+406
+00:28:35,040 --> 00:28:37,860
+دكاترة بدرسوكوا microbiology يبدو ان هم دكاترة
+
+407
+00:28:37,860 --> 00:28:41,920
+برضه بدرسوهم كيف يقاوموا يعني زي ما احنا بنخطط هم
+
+408
+00:28:41,920 --> 00:28:46,080
+برضه كمان بيخطط وكل ما بنكتشف antibiotic انا قولت
+
+409
+00:28:46,080 --> 00:28:51,160
+في ملايات المحاضرة الانسان ضن انه عنده يعني اكتر
+
+410
+00:28:51,160 --> 00:28:52,160
+من كفايته من
+
+411
+00:29:00,300 --> 00:29:05,080
+the last bullet مصبوع و هذا للأسف يعني كبار مرة
+
+412
+00:29:05,080 --> 00:29:09,580
+لسوء استخدام و سوء توظيف اللي هو المضادات الحاجة
+
+413
+00:29:09,580 --> 00:29:14,500
+أنا ده أقول مثال بقول بالألفين و خمسة عملنا دراسة
+
+414
+00:29:14,500 --> 00:29:20,560
+على أنت بنتك اسمه ميرو بنيم ميرو بنيم بيوصف بإنه
+
+415
+00:29:20,560 --> 00:29:23,240
+the proudest هو الأول
+
+416
+00:29:27,980 --> 00:29:31,380
+وبالفعل في ال 2005 لما عزلنا البكتيريا من مستشفى
+
+417
+00:29:31,380 --> 00:29:33,760
+الشفاء اللى هو المفروض انه resistant يعني ولا
+
+418
+00:29:33,760 --> 00:29:36,360
+بكتيريا كانت resistant للميروبنين كلهم sensitive
+
+419
+00:29:36,360 --> 00:29:40,080
+كل اللى عزلناهم كانوا sensitive للميروبنين بعد أقل
+
+420
+00:29:40,080 --> 00:29:43,620
+من 3 سنوات او 4 سنوات قمنا نعمل دراسة شبيهة واخد
+
+421
+00:29:43,620 --> 00:29:48,080
+لعيلات من مستشفى الشفاء بلغة تظهر ان 3% 5% 7%
+
+422
+00:29:48,080 --> 00:29:52,320
+resistance للميروبنين هذا مؤشر على ان احنا يعني ..
+
+423
+00:29:52,320 --> 00:29:55,480
+يعني خلال .. اذا كانت أقل من 3 سنوات صار لنا هذا
+
+424
+00:29:55,480 --> 00:29:59,360
+ال percentageتتوقع كله .. خد بالك .. الزيادة
+
+425
+00:29:59,360 --> 00:30:03,280
+متكونش تلاتة .. ستة .. تسعة .. لأ الزيادة بتكون
+
+426
+00:30:03,280 --> 00:30:07,280
+ممكن قفزات .. ممكن تكون قفزات وبالتالي بتوقع انه
+
+427
+00:30:07,280 --> 00:30:10,600
+خلال اقل من تلتة اربع سنوات برضه الملابنين يصيبوا
+
+428
+00:30:10,600 --> 00:30:13,720
+زي وزي لهم مكسلين او لانبسلين القديم اللي .. اللي
+
+429
+00:30:13,720 --> 00:30:19,140
+زمان البكير قامت لسوء الاستخدام .. لسوء الاستخدام
+
+430
+00:30:19,140 --> 00:30:21,560
+.. وسوء الاستخدام اللي هو أشكال متنوعة ممكن نتكلم
+
+431
+00:30:21,560 --> 00:30:26,350
+عنها في نهاية المحاضرة القادمةالنهاية المحاضرة
+
+432
+00:30:26,350 --> 00:30:29,650
+القادمة هتكون فيها اكتر من شغل انا قولتلكوا
+
+433
+00:30:29,650 --> 00:30:32,450
+هتقعدوا هتفرجوا هتكونوا اتفرجتوا في الفيديوهات
+
+434
+00:30:32,450 --> 00:30:36,070
+وانا هشرح اندي انيمايشن برضه توضح ال mechanism of
+
+435
+00:30:36,070 --> 00:30:40,150
+action لبعض ال antibiotics وهتطرق ل how to test
+
+436
+00:30:40,150 --> 00:30:43,670
+for the sensitivity of this antibiotic وما هي
+
+437
+00:30:43,670 --> 00:30:48,590
+الطرق اللي بتستخدمها البكتيريا في مقاومة الموضوع
+
+438
+00:30:48,590 --> 00:30:55,800
+الحيوانيأحنا اتفضنا أنه تصنيف الأساسي عنا كان مبني
+
+439
+00:30:55,800 --> 00:30:58,780
+على ال site affected اللي هو ال cell wall أو ال
+
+440
+00:30:58,780 --> 00:31:00,900
+cell membrane حتتبقى فيه أول مجموعة اللي هي
+
+441
+00:31:00,900 --> 00:31:04,640
+inhibitors of cell wall synthesis اللي منها ال
+
+442
+00:31:04,640 --> 00:31:09,520
+penicillin اللي قسمناه لقسمين natural penicillin و
+
+443
+00:31:09,520 --> 00:31:13,120
+semi-synthetic penicillin بما أن أنا مشاركتكم
+
+444
+00:31:13,120 --> 00:31:19,500
+وتفاعلكم لما أنا عندي penicillin natural لماذا قد
+
+445
+00:31:19,500 --> 00:31:24,700
+أرغبفي تحويله إلى او اعمله modification، بتعمله
+
+446
+00:31:24,700 --> 00:31:31,640
+تحوير او تطويره عشان
+
+447
+00:31:31,640 --> 00:31:40,060
+احسن منه، يعني مقاومة اكتر، زيادة الفعاليات، ايش
+
+448
+00:31:40,060 --> 00:31:42,980
+كمان؟ اللي ممكن انكبتله لإن في الدنياكيف يعني
+
+449
+00:31:42,980 --> 00:31:48,520
+الكمية؟ يعني بدل .. بدل ما أنتش انا طول ان أنتش
+
+450
+00:31:48,520 --> 00:31:51,860
+طول ان انا اعمل مثلا؟ او يكون sensitive اكتر يعني
+
+451
+00:31:51,860 --> 00:31:58,060
+انا نفس الاشي فعالي ايش كده؟ مثلا كمية مثلا أقل من
+
+452
+00:31:58,060 --> 00:32:01,700
+.. أقل من .. sight effects اقل من sight effects
+
+453
+00:32:01,700 --> 00:32:08,560
+مثلا طبعا ايش كده؟ يعني فعالي فعالي معاكطب انا ..
+
+454
+00:32:08,560 --> 00:32:11,880
+انا .. انا .. انا سأل سؤال يعني يمكن يكون في سبب
+
+455
+00:32:11,880 --> 00:32:16,000
+برضه، لو احنا مرضنا باللوازم، نفضل ناخد إبارح ولا
+
+456
+00:32:16,000 --> 00:32:20,240
+ناخد حبوب؟طيب يعني ممكن انا اعمل modification ل
+
+457
+00:32:20,240 --> 00:32:22,980
+drug عشان اغير الشكل الدوائي تبعه و الجرعه ليش
+
+458
+00:32:22,980 --> 00:32:25,020
+هتغيره؟ لازم يوضعوا إفره أصلا و لا يوضعوش أثبوب
+
+459
+00:32:25,020 --> 00:32:31,060
+البنسالين لأنه لا ينطص في الأمعاء لأ مش انهم يحبوا
+
+460
+00:32:31,060 --> 00:32:35,880
+ع زهورهم لأ لإن هو لا ينطص في الأمعاء فلو غيرنا و
+
+461
+00:32:35,880 --> 00:32:38,040
+أحنا بالفعل غيرنا و أخدنا ال unbicillin و
+
+462
+00:32:38,040 --> 00:32:41,120
+الamoxicillin مشتقات من البنسالين نفسنا ناخدهم
+
+463
+00:32:41,120 --> 00:32:47,210
+كبسولات all لإن التعديل صار قابل للانتصاصلكن ال
+
+464
+00:32:47,210 --> 00:32:50,690
+anbicillin بناخده أربع حتبات في اليوم و ال
+
+465
+00:32:50,690 --> 00:32:53,390
+amoxicillin بناخده تلت حتبات في اليوم عارفين ليش؟
+
+466
+00:32:53,390 --> 00:32:56,390
+لإن برضه ال absorbability ذات ال .. ال amoxicillin
+
+467
+00:32:56,390 --> 00:33:00,310
+أحسن من مين؟ من ال anbicillin فهناك طرح فتاخده
+
+468
+00:33:00,310 --> 00:33:04,410
+أربع مرات أو تاخده تلت مرات يبقى كمان .. شوف ال ..
+
+469
+00:33:04,410 --> 00:33:09,110
+ال .. ال modification انت عارفين أفضل drug .. أفضل
+
+470
+00:33:09,110 --> 00:33:12,050
+drug إنه ناخده مرة واحدة في .. مرة واحدة و .. و
+
+471
+00:33:12,050 --> 00:33:16,720
+خلاص ماخدوش بعدين حتى كمانبعد عشر أهلكم مصطلح اسمه
+
+472
+00:33:16,720 --> 00:33:21,180
+patient convenience patient convenience و patient
+
+473
+00:33:21,180 --> 00:33:26,440
+compliance convenience و compliance convenient رضا
+
+474
+00:33:26,440 --> 00:33:33,180
+للمريض satisfaction تبع المريض بوجه بوجهش طبعا مهم
+
+475
+00:33:33,180 --> 00:33:36,460
+هذا مهم مهم مهم في ال compliance ايش هي ال
+
+476
+00:33:36,460 --> 00:33:42,080
+compliance مدى التزام للمريضأنا كتبت وصفة طبية
+
+477
+00:33:42,080 --> 00:33:47,660
+لمريض تلت قبر على مدار تلت أيام كل يوم قبر الصبح
+
+478
+00:33:47,660 --> 00:33:52,520
+هذا المريض بلا أدم إله حياة و هصحى إنه لأ بيقدر وع
+
+479
+00:33:52,520 --> 00:33:57,140
+الشغل و حتى عنده tonsillitis بطلع من رفح الساعة
+
+480
+00:33:57,140 --> 00:34:00,760
+سبعة بيجي بيصل الجامعة تمانية بروح رفح الساعة خمسة
+
+481
+00:34:00,760 --> 00:34:04,440
+عشان ياخد إيه بلاوة بده يروح بده يروح على ممرض أو
+
+482
+00:34:04,440 --> 00:34:06,600
+على أيادة أو على .. مش هيعملها في البيت صح ده
+
+483
+00:34:06,600 --> 00:34:08,800
+فبتاقوا مؤلمة بقولك
+
+484
+00:34:13,160 --> 00:34:15,920
+ما هو compliance؟ مش compliance، لكن لو كانت
+
+485
+00:34:15,920 --> 00:34:18,780
+كابسولة بدل ال injection، طب ما هو بياخدها في أي
+
+486
+00:34:18,780 --> 00:34:21,760
+وقت فرار بين محاضرة ومحاضرة، مال يبقى فيه كاس ميه
+
+487
+00:34:21,760 --> 00:34:24,920
+وبيشتغل ويحضر؟ لأ، يبقى ال patient convenience
+
+488
+00:34:24,920 --> 00:34:26,880
+يؤدي إلى ال patient compliance
+
+489
+00:34:31,390 --> 00:34:34,790
+جزء من الهدايا فشل، ابقى عايز أحسن فيه انتكاسة،
+
+490
+00:34:34,790 --> 00:34:38,410
+وفي نفس الواجهة، تذكروا إن أنا فيها بكتيري يهتكت
+
+491
+00:34:38,410 --> 00:34:41,990
+بالـ antibiotic بـconcentration لم تصل إلى فترة
+
+492
+00:34:41,990 --> 00:34:43,770
+طويلة تقتل البكتيري وقتها، اللي ممكن تكون
+
+493
+00:34:43,770 --> 00:34:44,730
+resistance
+
+494
+00:34:50,700 --> 00:34:53,880
+بيقدّي ل compliance هذا الكلام مفهوم في المستشفى
+
+495
+00:34:53,880 --> 00:34:56,700
+لو أنا نايم في المستشفى و معالجي اللي محلول يعطوني
+
+496
+00:34:56,700 --> 00:34:58,620
+انجليش مش مش مش مش مش مش مش مش مش مش مش مش مش مش
+
+497
+00:34:58,620 --> 00:34:59,500
+مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش
+
+498
+00:34:59,500 --> 00:34:59,520
+مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش
+
+499
+00:34:59,520 --> 00:34:59,940
+مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش
+
+500
+00:34:59,940 --> 00:35:00,040
+مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش
+
+501
+00:35:00,040 --> 00:35:02,560
+مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش
+
+502
+00:35:02,560 --> 00:35:02,880
+مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش
+
+503
+00:35:02,880 --> 00:35:04,680
+مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش
+
+504
+00:35:04,680 --> 00:35:04,680
+مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش
+
+505
+00:35:04,680 --> 00:35:06,580
+مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش مش
+
+506
+00:35:06,580 --> 00:35:17,060
+مش مش مش
+
+507
+00:35:17,060 --> 00:35:21,780
+مش مش مشلأسباب متلوعة ذكرته مجموعة منها وعلى ما
+
+508
+00:35:21,780 --> 00:35:24,760
+أنصح انه ندور على أسباب أخرى كمان نفكر في أسباب
+
+509
+00:35:24,760 --> 00:35:31,200
+أخرى منها ال .. ال .. ال .. to extend the spectrum
+
+510
+00:35:31,200 --> 00:35:35,260
+منها to combat resistance منها to improve quality
+
+511
+00:35:35,260 --> 00:35:38,620
+.. to remove side effects to be more safer زي ما
+
+512
+00:35:38,620 --> 00:35:43,180
+حكينا أو لتغيير الجرعة الدوائية من injection إلى
+
+513
+00:35:43,180 --> 00:35:47,780
+مثلا ولا إلا آخرلذلك ضغط عندي natural penicillin و
+
+514
+00:35:47,780 --> 00:35:52,300
+ضغط عندي semi-synthetic penicillin، semi-synthetic
+
+515
+00:35:52,300 --> 00:35:54,920
+معناها إنه أنا أخدت ال natural و عملتله
+
+516
+00:35:54,920 --> 00:36:00,380
+modification، مش completely synthetic، يعني ما
+
+517
+00:36:00,380 --> 00:36:03,880
+صنعت من السفر، أنتجنا بنسالين من الفطر و بعدين
+
+518
+00:36:03,880 --> 00:36:05,480
+أخدنا .. عملنا له modification
+
+519
+00:36:13,920 --> 00:36:17,580
+برضه كمان الكيميائيين اللى احنا بيشوفهم بيصنعوا
+
+520
+00:36:17,580 --> 00:36:23,040
+مركبات هدول علهم يعني ملكات خاصة في تخيل المركبات
+
+521
+00:36:23,040 --> 00:36:28,980
+وخصائصها فلعبوا مركب الأول قبل عن حرقة وجنبها CH2
+
+522
+00:36:28,980 --> 00:36:34,960
+وبعدين common nucleus نواة كيميائية مشتركة في كل
+
+523
+00:36:34,960 --> 00:36:39,000
+الفنسينات كل الفنسينات فيها نفس ال .. النواة هذه
+
+524
+00:36:39,810 --> 00:36:45,450
+ايش الافتلاف بين الأول والتاني CH2 والتاني OCH2؟
+
+525
+00:36:45,450 --> 00:36:49,550
+هداك الأولاني مستحيل الجسم يمتص منه milligram
+
+526
+00:36:49,550 --> 00:36:55,450
+والتاني بيمتص منه عادي، يعني تغير ال absorbance
+
+527
+00:36:55,450 --> 00:36:59,890
+تماما لل Antibiotic اللي اسمه Benzaline فقط بإضافة
+
+528
+00:36:59,890 --> 00:37:03,490
+Oxygen مدان ال CH2 اللي في ال .. في ال side chain
+
+529
+00:37:04,630 --> 00:37:09,010
+متخيلين ولا كيف؟ اذا اي صلعة ت change قد يؤدي الى
+
+530
+00:37:09,010 --> 00:37:12,830
+dramatic change في الخصائص المركبات مش هتقول الله
+
+531
+00:37:12,830 --> 00:37:17,710
+انه لذلك .. لذلك بنقول الكيميائين عندهم مقدرة على
+
+532
+00:37:17,710 --> 00:37:21,250
+إضافة درّة كلور .. تلت درّات كلور زي كلور
+
+533
+00:37:21,250 --> 00:37:26,410
+تترسيكلين تمام؟ ففي عندهم .. عندهم المقدرة على
+
+534
+00:37:26,410 --> 00:37:29,950
+تغيير المركبات وبتالي تغيير خصائص هذه المركبات
+
+535
+00:37:29,950 --> 00:37:33,230
+المشكلة ان في غزة احنا بنصنع مركبات حقيقة يعني انا
+
+536
+00:37:33,230 --> 00:37:40,750
+الكيميائين الأخوة الليهي قدرة تنافس قدرات العالم
+
+537
+00:37:40,750 --> 00:37:44,210
+كله في موضوع التصنيع لكن ماعناش مقدرة على انه نعمل
+
+538
+00:37:44,210 --> 00:37:49,710
+testing لها دي الملكبات مدى فعاليتها بشكل جيد في
+
+539
+00:37:49,710 --> 00:37:55,950
+عندي حاجة اسمها oxasilin وهو resistant to
+
+540
+00:37:55,950 --> 00:37:58,690
+penicillinase مكتوب على الانبوسين resistant to
+
+541
+00:37:58,690 --> 00:38:03,040
+penicillinase ايش ال penicillinase؟البنسيلينيز هو
+
+542
+00:38:03,040 --> 00:38:07,560
+الانزيم اللى بيكسر البنسيلين اتطلعوا على البنسيلين
+
+543
+00:38:07,560 --> 00:38:12,000
+يقولون ان هذى .. هذى .. اذا قولتها في مراقبتيها
+
+544
+00:38:12,000 --> 00:38:16,360
+فاسمها بنسيلين لكن هذى الجزء اللى هذا شايفينه اللى
+
+545
+00:38:16,360 --> 00:38:20,020
+هى قامنين انا نساهم عليها اسمها بيتالاكتم رينج
+
+546
+00:38:20,020 --> 00:38:25,980
+البيتالاكتم رينج ال بنسيلينيز بيستهدفها بما ال cut
+
+547
+00:38:25,980 --> 00:38:32,540
+فيها cutبصير البنسالين غير قادر على إحداث ال
+
+548
+00:38:32,540 --> 00:38:38,180
+action تبعه بالمرة، بفقد قدرته، تمام؟ يبقى ممكن
+
+549
+00:38:38,180 --> 00:38:43,320
+نسميه penicillinase أو بنسميه beta-lactamase عشان
+
+550
+00:38:43,320 --> 00:38:45,740
+ذلك ال antibiotics بيسموها beta-lactam-antibiotic
+
+551
+00:38:45,740 --> 00:38:48,700
+واحدة لحياة، ومعها بيسموها penicillins اللي اسم
+
+552
+00:38:48,700 --> 00:38:54,100
+الكيميا، إذن بنتخيل مع بعض كانت التالي إنه بكتيريا
+
+553
+00:38:54,100 --> 00:39:00,460
+أندجت penicillinase بيكسر البنسالينفاحنا حورنا ال
+
+554
+00:39:00,460 --> 00:39:02,720
+penicillin تبعنا و خلناه oxycelin بدل ما هو
+
+555
+00:39:02,720 --> 00:39:07,700
+penicillin عشان مايتكسرش، لكن البكتيريا وين؟ على
+
+556
+00:39:07,700 --> 00:39:10,280
+مين؟ على البكتيريا عندهم برضه و لما organic
+
+557
+00:39:10,280 --> 00:39:15,160
+chemistry أشتر من تبعوننا، طبعا؟ لذلك لما بنقول
+
+558
+00:39:15,160 --> 00:39:17,760
+penicillinase غالبا ما بنقولش penicillinase و
+
+559
+00:39:17,760 --> 00:39:22,020
+بنسكت، بنقول penicillinasesبمعنى ان البكتيريا
+
+560
+00:39:22,020 --> 00:39:26,720
+ستنتج أنواع مختلفة من ال Penicillinases عشان تكافح
+
+561
+00:39:26,720 --> 00:39:30,520
+كل Penicillin احنا نعمله Synthesis جديد هي برضه
+
+562
+00:39:30,520 --> 00:39:35,980
+بتطور من قدرتها و تنتج Penicillin الاخير في
+
+563
+00:39:35,980 --> 00:39:39,900
+القائمة اللي هو ال Anbicillin واضح بين بثانيه انه
+
+564
+00:39:39,900 --> 00:39:44,020
+Extended Spectrum بقى فقط المنسلين الرامبوسة بينما
+
+565
+00:39:44,020 --> 00:39:47,120
+ال Anbicillin بيستخدموله ال E.coli في ال .. في ال
+
+566
+00:39:47,120 --> 00:39:54,940
+urinary tract infection ال E.coli أشهيةيبقى فيها
+
+567
+00:39:54,940 --> 00:40:00,820
+أكتر من سبب للعب لللعب في المراكبات وتحويلها أو
+
+568
+00:40:00,820 --> 00:40:01,600
+تحويلها
+
+569
+00:40:06,120 --> 00:40:09,180
+في ال .. في ال .. في ال drugs مش على اكتشاف ال new
+
+570
+00:40:09,180 --> 00:40:12,680
+drugs و ان انا على modification لل existing drug
+
+571
+00:40:12,680 --> 00:40:17,040
+ليش؟ لأن ال existing drugs غالبا جرّبك و هي safe،
+
+572
+00:40:17,040 --> 00:40:22,240
+أه؟ فبالتالي ال modification قد تعدل .. تحسن بما
+
+573
+00:40:22,240 --> 00:40:24,900
+لا .. يعني في .. في .. في معروف الحياة
+
+574
+00:40:29,910 --> 00:40:34,630
+صعب الصورة هذه، اي واحد بيطلع فيها، يعني بيقول ان
+
+575
+00:40:34,630 --> 00:40:39,370
+ما بيسمها الصحيح، ماكانش يعني موفق، لإن لو ركز
+
+576
+00:40:39,370 --> 00:40:44,150
+فيها دقائق محدودة، ممكن يعرف منها بعض المعلومات،
+
+577
+00:40:44,150 --> 00:40:50,230
+اللي بنوع الأحمر، واحد، اتنين، ثلاثة، اربع
+
+578
+00:40:53,830 --> 00:40:57,710
+هما تسعة اصناف من ال antibiotics كلهم بيشتغلوا على
+
+579
+00:40:57,710 --> 00:41:04,510
+ال cell wall لكن كلهم بيشتغلوا على different steps
+
+580
+00:41:04,510 --> 00:41:10,670
+من ال cell wall برضه هذه نقطة مهمة انها زي ما قلت
+
+581
+00:41:10,670 --> 00:41:13,630
+لك في ال protein synthesis okay they inhibit
+
+582
+00:41:13,630 --> 00:41:15,850
+protein synthesis لكن برضه with different
+
+583
+00:41:15,850 --> 00:41:19,090
+mechanism of ايه of action كذلك بالنسبة لها ال
+
+584
+00:41:19,090 --> 00:41:24,180
+cellطيب هنبرخص الكلام اللي حكيناه Penicillin,
+
+585
+00:41:24,240 --> 00:41:26,920
+Penicillinase, Resistant Penicillins, Extended
+
+586
+00:41:26,920 --> 00:41:30,600
+Spectrum Penicillins الرابعة لأ ماذكرناهاش الرابعة
+
+587
+00:41:30,600 --> 00:41:34,740
+و هنشرحها لأ Penicillins زائد Beta-lactam, Beta
+
+588
+00:41:34,740 --> 00:41:38,400
+-lactams in it تلقوا معايا الآن على المثال اللي
+
+589
+00:41:38,400 --> 00:41:44,500
+بتحكيه هي البكتيريا و معها صيف ايش صيفها لأ ال
+
+590
+00:41:44,500 --> 00:41:50,460
+Beta-lactamaseعطينا المريض انتيباتك اسمه بنسالين
+
+591
+00:41:50,460 --> 00:41:54,540
+فالبكتيريا بتقول لبنسالين روح تعامل معاه، روح
+
+592
+00:41:54,540 --> 00:41:59,660
+تعامل معاه فال بنسالينيز بيكسر البنسالين، يبقي
+
+593
+00:41:59,660 --> 00:42:04,680
+البنسالين صار useless فالإنسان مش عامل، جاب
+
+594
+00:42:04,680 --> 00:42:09,780
+انتيباتك بنسالين و جاب معاه inhibitor للبنسالينيز
+
+595
+00:42:11,210 --> 00:42:13,370
+فصار ال Antibiotic لما يشوف البنسيلنايزي يقولله
+
+596
+00:42:13,370 --> 00:42:16,330
+الليه؟ يقولله ال .. ال .. ال .. ال inhibitor بتبقى
+
+597
+00:42:16,330 --> 00:42:19,830
+هو متعامل مع مين؟ مع ال .. ال .. ال enzyme
+
+598
+00:42:19,830 --> 00:42:22,450
+البنسيلنايزي هو صار neutral really مين البكتيريا
+
+599
+00:42:22,450 --> 00:42:29,030
+sensitive لبنسيلنايزي و أشهر drug انا في غزة مع
+
+600
+00:42:29,030 --> 00:42:31,670
+موظفكوا بتعرفوا اسمه Augmentin
+
+601
+00:42:36,140 --> 00:42:41,060
+من Amoxicillin زائد Clavunelic Acid ال Amoxicillin
+
+602
+00:42:41,060 --> 00:42:45,800
+هو Penicillin وال Clavunelic Acid هو Penicillinase
+
+603
+00:42:45,800 --> 00:42:50,200
+Inhibitor Penicillinase Inhibitor وبالتالي ارجع او
+
+604
+00:42:50,200 --> 00:42:54,480
+رجعنا حساسية ال Penicillin عن طريق بعض لذلك
+
+605
+00:42:54,480 --> 00:42:57,300
+بالنسبالي ان ال Augmentin is more expensive than
+
+606
+00:42:57,300 --> 00:43:00,240
+Amoxicillin a lot لأن بالفعل Amoxicillin غالب
+
+607
+00:43:00,240 --> 00:43:04,590
+الأوقات is useless تقريبابالطبع ليس كل الأحوال
+
+608
+00:43:04,590 --> 00:43:09,750
+بينما الـ augmentation is more effective وفي نقطة
+
+609
+00:43:09,750 --> 00:43:13,990
+أريد أن أركز عليها شائعة خطأ شائعة واللي للأسف
+
+610
+00:43:13,990 --> 00:43:18,470
+يعني اللي بيروح لهم الأطباء اللي هم يقولونك يبتدلك
+
+611
+00:43:18,470 --> 00:43:22,310
+أنت بيتك قوي لا يولد بلد حد اسمه أنت بيتك قوي و
+
+612
+00:43:22,310 --> 00:43:26,570
+أنت بيتك ضعيف يولد أنت بيتك مناسب و أنت بيتك غير
+
+613
+00:43:26,570 --> 00:43:33,470
+مناسب كلمة قاوميمريح على المريض، مريح على المريض
+
+614
+00:43:33,470 --> 00:43:37,410
+بيعطيه إنه لاخده الله مضال حياة قوي، لأ، لأ، لسه
+
+615
+00:43:37,410 --> 00:43:42,830
+للأسف، للأسف أساءت و سيئة استخدامها وبالتالي صاروا
+
+616
+00:43:42,830 --> 00:43:46,270
+يطلقوا على ال broad spectrum إنه إيه أنت بقيت
+
+617
+00:43:46,270 --> 00:43:51,070
+القوي، فصار المريض ياخد ال broad spectrum و يشفى
+
+618
+00:43:51,070 --> 00:43:53,210
+من المرض طبعه بسهولة و بسهولة
+
+619
+00:43:55,670 --> 00:43:58,350
+الاخرى نتيجة عن الخلل اللى حكينا عنه في ال normal
+
+620
+00:43:58,350 --> 00:44:02,730
+أفلام أستاذ بس هلأ الشىء اللى بيخلي البكتيريا تروح
+
+621
+00:44:02,730 --> 00:44:20,290
+لإنهيبترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمترمتر
+
+622
+00:44:21,290 --> 00:44:24,470
+ال .. ال .. ال inheritor هيرتبط بمين؟ لما .. لو ..
+
+623
+00:44:24,470 --> 00:44:27,890
+لو .. في مجموعة هيرتبط في .. في .. في mixture ال
+
+624
+00:44:27,890 --> 00:44:30,870
+.. ال inheritor هيرتبط بال باليزينايز و ال anthrac
+
+625
+00:44:30,870 --> 00:44:34,150
+هيرتبط بمين؟ بال بكتيريا بال بكتيريا بال بكتيريا
+
+626
+00:44:34,150 --> 00:44:38,170
+بال بكتيريا في كلمة class اسمه Carb Abnames و هذا
+
+627
+00:44:38,170 --> 00:44:41,250
+اللي قلت عنه قبل شوية انا اللي منه ال merabname
+
+628
+00:44:41,250 --> 00:44:45,740
+اللي هو the broadest spectrum of allالصورة طبعا
+
+629
+00:44:45,740 --> 00:44:49,080
+البنسالين والبنسالينيز شوفوا التكسير اللى صار فى
+
+630
+00:44:49,080 --> 00:44:52,400
+ال beta lactam ring فقط فى المنطقة دى صار بنسلوك
+
+631
+00:44:52,400 --> 00:44:55,440
+أسد او بنسلوك أسد فقط القدر على انه يعمل
+
+632
+00:44:55,440 --> 00:44:58,040
+inhibition للبرتيريا او killing للبرتيريا ونكمل ان
+
+633
+00:44:58,040 --> 00:44:59,320
+شاء الله الموضوع القادر نفس العملي
+
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/D_0va0lT7Sw.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/D_0va0lT7Sw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..80adbbd6ce8f3c85cb2286da4263859f7eaee13a
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/D_0va0lT7Sw.srt
@@ -0,0 +1,2799 @@
+1
+00:00:20,620 --> 00:00:23,720
+بسم الله الرحمن الرحيم اتوقفنا المرة الماضية عند
+
+2
+00:00:23,720 --> 00:00:27,140
+الستربتوكوكاس نيمونيا شرحنا بعض النقاط المتعلقة فيها
+
+3
+00:00:27,140 --> 00:00:30,560
+لأنّه يتم التركيز عليها مروّجيا إن شاء الله قولنا هي
+
+4
+00:00:30,560 --> 00:00:35,140
+جرام بوستيف دبلوكوكاي زيها زي الستربتوكوكاي
+
+5
+00:00:35,140 --> 00:00:40,920
+كتاليز نيجاتيف ولا بوستيف؟ نيجاتيف هذه الصفة
+
+6
+00:00:40,920 --> 00:00:46,140
+الأساسية نيجاتيف قلنا عنها أنّها دبلو كوكاي وحتى
+
+7
+00:00:46,140 --> 00:00:48,700
+الشكل الكوكاي اللي عن طبق عليها حقيقة قلنا أنّها
+
+8
+00:00:48,700 --> 00:00:53,140
+تشبه الكوكوبسلاي أكثر لكن يعني شبهناها بكلمة القدم
+
+9
+00:00:53,140 --> 00:00:55,880
+الأمريكية اللي هي البيضاوية جنب بعض
+
+10
+00:00:55,880 --> 00:01:00,900
+بالإضافة إلى وجود capsule قضينا
+
+11
+00:01:00,900 --> 00:01:04,560
+يعني جلّ المحاضرة السابقة تحدثت عن الكبسول وعن
+
+12
+00:01:04,560 --> 00:01:07,600
+اليومالايسين وبعض ال service protein والتيكويك أسيد
+
+13
+00:01:08,060 --> 00:01:11,160
+و الـ .. بيبتيدوجلايكان fragments وكيف تأثيرهم
+
+14
+00:01:11,160 --> 00:01:15,580
+على جهاز المناعة وتحدثنا عن حاجة اسمها auto
+
+15
+00:01:15,580 --> 00:01:19,740
+لايسين auto-license بعدين فرضنا في الـ .. الـ ..
+
+16
+00:01:19,740 --> 00:01:24,600
+الصفحة كاملة لحديث عن الكابسول واستفدنا من
+
+17
+00:01:24,600 --> 00:01:27,480
+الكابسول مجموعة من المعلومات الصحية قلنا أنّ الـ
+
+18
+00:01:27,480 --> 00:01:30,940
+فاكسينايشن احنا بنساعد بالكابسول بالـ vaccination
+
+19
+00:01:30,940 --> 00:01:36,300
+وفي الـ identification برضه بنساعده من؟ الكابسول في
+
+20
+00:01:36,300 --> 00:01:39,860
+الـ vaccination قلنا أنّنا مشكلة حقيقية 84 serotype
+
+21
+00:01:39,860 --> 00:01:44,540
+فينا وبالتالي مش منطقي نعطي واحد vaccine بيحتوي
+
+22
+00:01:44,540 --> 00:01:50,900
+على 84 serotype لذلك بندور في البلد الـ most common
+
+23
+00:01:50,900 --> 00:01:54,020
+الـ .. بالظبط الـ most common serotypes وبنجمعهم و
+
+24
+00:01:54,020 --> 00:01:56,880
+بنعطي vaccine لهم ففي واحد مشهور بـ 14
+
+25
+00:01:56,880 --> 00:02:01,830
+serotypes يعني يحتوي على 14 serotypes في الـ
+
+26
+00:02:01,830 --> 00:02:05,310
+identification عشان أنا أعرف مين الـ common مش لازم
+
+27
+00:02:05,310 --> 00:02:09,290
+برضه نعمل لما بعزل الستربتوكوكاس نيمونيا أتعرف على
+
+28
+00:02:09,290 --> 00:02:12,710
+زيرو تايب وبالتالي لازم أستخدم برضه specific
+
+29
+00:02:12,710 --> 00:02:16,830
+antiserum لتعرف على اللي هو الـ capsular zero types
+
+30
+00:02:16,830 --> 00:02:22,470
+آخر جملة كانت اللي ركزنا عليها ودلوقتي اللي هي only
+
+31
+00:02:22,470 --> 00:02:26,390
+for susceptible population ما معنى susceptible
+
+32
+00:02:26,390 --> 00:02:31,230
+population خليني أوضح بس المفهوم عشان منا ماننسوش
+
+33
+00:02:32,300 --> 00:02:37,660
+تسمع بمرض يصيب الأطفال، مرض يصيب الكبار السن، مرض
+
+34
+00:02:37,660 --> 00:02:42,120
+يصيب فقط مرضى الـ aids، مرض لا يصيب اللي عندهم
+
+35
+00:02:42,120 --> 00:02:48,710
+أنيميا الملاريا مثلا ما تصيبش الناس اللي عندهم أنيميا
+
+36
+00:02:48,710 --> 00:02:52,550
+الخلايا المنجلية sickles anemia تمام؟ يبقى في
+
+37
+00:02:52,550 --> 00:02:56,090
+susceptible groups وفي non susceptible groups
+
+38
+00:02:56,090 --> 00:03:00,750
+اللي احنا المرة اللي فاتت قولناها يعني زي الـ host
+
+39
+00:03:00,750 --> 00:03:04,430
+specificity على سبب المثال لكن هنا بنتكلم عن
+
+40
+00:03:04,430 --> 00:03:09,090
+الإنسان هو كله susceptible لكن في فئات أكثر عرضة
+
+41
+00:03:09,090 --> 00:03:12,750
+للإصابة مثلا احنا في مجال التحديات الطبية في
+
+42
+00:03:12,750 --> 00:03:17,190
+المستشفيات are susceptible group to be infected
+
+43
+00:03:17,190 --> 00:03:22,770
+with blood-borne viruses زي مثلا الـ hepatitis B و
+
+44
+00:03:22,770 --> 00:03:25,710
+الـ hepatitis C على سبيل المثال تمام؟ blood-borne
+
+45
+00:03:25,710 --> 00:03:29,410
+diseases مش أكثر وضع من الغاز والإيبارا بتاع ليه؟
+
+46
+00:03:29,410 --> 00:03:33,150
+احنا أكثر وضع للإصابة تكملة susceptible population
+
+47
+00:03:33,150 --> 00:03:37,630
+هم للفئة الأكثر عرضة الأكثر عرضة قد يكون الأطفال
+
+48
+00:03:37,630 --> 00:03:39,430
+قد يكون مرضى بمرض معين آخر
+
+49
+00:03:42,990 --> 00:03:45,770
+احنا تحدثنا عن الـ Streptococcus pneumonia وعن الـ
+
+50
+00:03:45,770 --> 00:03:48,350
+violence factors قبل أن نتحدث عن الأمور اللاحقة هي
+
+51
+00:03:48,350 --> 00:03:52,210
+المسمى تبعها الـ disease pneumonia و looper
+
+52
+00:03:52,210 --> 00:03:54,810
+pneumonia فاكرين looper pneumonia لكن بتكونوا
+
+53
+00:03:54,810 --> 00:03:58,790
+تعرفوا أنّها غالبا مش هي الـ primary cause of
+
+54
+00:03:58,790 --> 00:04:04,010
+pneumonia إيش هي الـ primary cause؟ يعني في بعض
+
+55
+00:04:04,010 --> 00:04:07,410
+الحاجة أنّها فيها ممكن يكون secondary cause أعطيكم
+
+56
+00:04:07,410 --> 00:04:11,810
+مثال بسيط  لأن عندنا واحد مرض بالـ influenza والـ
+
+57
+00:04:11,810 --> 00:04:15,330
+influenza هي upper respiratory tract infection و
+
+58
+00:04:15,330 --> 00:04:17,770
+قد تصل إلى اللي هو الـ respiratory tract في الـ
+
+59
+00:04:17,770 --> 00:04:19,890
+upper respiratory tract بيصير فيه destruction للـ
+
+60
+00:04:19,890 --> 00:04:22,870
+epithelial cells إذا صارت destruction للـ
+
+61
+00:04:22,870 --> 00:04:25,710
+epithelial cells للستربتوكوكاس نيمونيا اللي
+
+62
+00:04:25,710 --> 00:04:30,490
+كانت موجودة normal flora في أفواهكم ممكن تعمل باطل
+
+63
+00:04:30,490 --> 00:04:31,290
+بعدين
+
+64
+00:04:42,240 --> 00:04:46,240
+مين ممكن يصاب أو يسلم الـ destruction للـ
+
+65
+00:04:46,240 --> 00:04:51,960
+respiratory epithelial cells ممكن اللي عندهم viral
+
+66
+00:04:51,960 --> 00:04:55,540
+infection اللي عندهم viral infection المدخنين
+
+67
+00:04:55,540 --> 00:04:58,180
+يسمونهم الـ chain smokers اللي هم الـ heavy smokers
+
+68
+00:04:58,180 --> 00:05:03,920
+اللي بيدخنوا بشكل كبير من يعملون في مصانع فيها
+
+69
+00:05:03,920 --> 00:05:07,700
+irritants إيش يعني irritants؟ مواد مهيجة بتسمعوا
+
+70
+00:05:07,700 --> 00:05:11,160
+الـ .. الـ .. الـ boya الـ formal .. الـ formal .. الـ
+
+71
+00:05:11,160 --> 00:05:16,080
+formaldehyde كثير من المركبات قد تسبب destruction
+
+72
+00:05:16,080 --> 00:05:19,040
+للخلايا وإن لابتكن الـ virus دولة لابتكن إيه؟
+
+73
+00:05:19,040 --> 00:05:22,960
+بكتيريا كل هؤلاء الناس بيكونوا أكثر عرضة للإصابة من
+
+74
+00:05:22,960 --> 00:05:25,360
+اللي هم الناس العاديين اللي ما عندهم أي إشكالية في
+
+75
+00:05:25,360 --> 00:05:30,120
+الـ upper respiratory tract ممكن تسبب أنّها Middle
+
+76
+00:05:30,120 --> 00:05:34,000
+Ear Infection ومشهور جدا كـ Middle Ear Infection
+
+77
+00:05:34,000 --> 00:05:38,480
+وبنسميها احنا دي Otitis Media Otitis Media التهاب
+
+78
+00:05:38,480 --> 00:05:43,580
+الأذن الوسطى التهاب الأذن الوسطى في أنّ الـ .. الـ ..
+
+79
+00:05:43,580 --> 00:05:49,240
+البكتيريميا تنتج بعد إصابة أولية مثلا pneumonia
+
+80
+00:05:49,240 --> 00:05:53,800
+أو Otitis Media إذا صار في بكتيريميا والرسم مجرى
+
+81
+00:05:53,800 --> 00:05:59,040
+بتخلص منها أو يتخلص من الميكروبات تتطور إلى
+
+82
+00:05:59,040 --> 00:06:03,760
+meningitis أنا قلتلكوا المحاضرة الماضية أنّ الـ
+
+83
+00:06:03,760 --> 00:06:10,660
+vaccines اللي استخدمت عملت أنّها حاجة إيجابية أنّها
+
+84
+00:06:10,660 --> 00:06:13,380
+قللت الإصابة بمرض الـ meningitis due to
+
+85
+00:06:13,380 --> 00:06:16,960
+streptococcus pneumonia نعيد من اللوحة نفسها الـ
+
+86
+00:06:16,960 --> 00:06:21,960
+clinical findings قلنا أنّ أشهر disease بسببه هو
+
+87
+00:06:21,960 --> 00:06:25,800
+pneumonia وقلنا غالبا هو عبارة عن secondary to
+
+88
+00:06:25,800 --> 00:06:28,760
+viral infections أو destruction by chemicals أو
+
+89
+00:06:28,760 --> 00:06:32,200
+irritants أخرى لكن هناك أمراض أخرى بتسببها
+
+90
+00:06:32,200 --> 00:06:35,200
+الستربتوكوكاس نيمونيا اللي من ضمنها الـ autitis media
+
+91
+00:06:35,200 --> 00:06:39,360
+والـ .. الـ bacterium and meningitis وقلنا أنّ الـ
+
+92
+00:06:39,360 --> 00:06:43,640
+vaccine .. الـ vaccine اللي وظف في الـ 2011 في قطاع
+
+93
+00:06:43,640 --> 00:06:48,600
+غزة سيعمل على خفض حالات من الـ meningitis اللي هي
+
+94
+00:06:48,600 --> 00:06:51,500
+أصلا غالبا ناتجة عن من؟ عن الـ pneumonia أو عن
+
+95
+00:06:51,500 --> 00:06:57,230
+autitis media في الأطفال من أشهر الفحوصات اللي
+
+96
+00:06:57,230 --> 00:07:00,970
+بنستخدمها لتعريف البكتيريا فحص mobile solubility
+
+97
+00:07:00,970 --> 00:07:05,590
+فاكرين الـ auto lysin لما البكتيريا بتشعر بتهديد
+
+98
+00:07:05,590 --> 00:07:08,970
+بتموت لحالها تقولنا كفى الله اللي ممكن نقتل ما حدش
+
+99
+00:07:08,970 --> 00:07:13,710
+يعني يعني يحاول أنا لحالي بدأت بالأمور الـ الـ الـ
+
+100
+00:07:13,710 --> 00:07:18,810
+bio معروف عنه معروف عنه أنّ عنده قدرة على قتل
+
+101
+00:07:18,810 --> 00:07:22,590
+البكتيريا والدليل على درجة أنّنا أضفناه على
+
+102
+00:07:22,590 --> 00:07:27,750
+المقنكي to inhibit the growth of gram positive طبعا
+
+103
+00:07:27,750 --> 00:07:29,650
+مش كل الـ gram positive شوفت في المحاضرة الماضية الـ
+
+104
+00:07:29,650 --> 00:07:32,550
+enterococcus تنمو في الـ bio تمام؟ بتقاوم الـ bio
+
+105
+00:07:32,550 --> 00:07:36,090
+لذلك كل من ينمو في الأمعاء غالبا لازم يكون عنده
+
+106
+00:07:36,090 --> 00:07:40,370
+نوع من المقاومة له الـ bio البكتيريا التنهيه مالهاش
+
+107
+00:07:40,370 --> 00:07:44,300
+أي مقاومة الصورتين اللي قد .. أو .. أو الأنبوبتين
+
+108
+00:07:44,300 --> 00:07:47,060
+اللي قدامكوا على اللوحة يا تركز على اللوحة واحدة
+
+109
+00:07:47,060 --> 00:07:48,560
+مكتوبة عليها positive واحدة مكتوبة عليها negative
+
+110
+00:07:48,560 --> 00:07:52,180
+الـ positive خالية تماما من النمو خالية تماما من الـ
+
+111
+00:07:52,180 --> 00:07:55,820
+turbidity بينما الـ negative مليئة بـ turbidity
+
+112
+00:07:55,820 --> 00:07:59,980
+ودليل على ذلك أنّ احنا شايفين الـ .. الـ أسلاك بتاعة الـ
+
+113
+00:07:59,980 --> 00:08:03,860
+back من خلالها في الـ positive ومش شايفين الأسلاك
+
+114
+00:08:03,860 --> 00:08:07,920
+في اللي هي الـ .. معنى ذلك هي فرع نبوبة ووضعنا
+
+115
+00:08:07,920 --> 00:08:13,380
+فيها بكتيريا واحدة من الاتنين اثنين أو أو أو عفوا
+
+116
+00:08:13,380 --> 00:08:17,060
+حاطين على واحدة منهم بال والتانية اللي حاطين
+
+117
+00:08:17,060 --> 00:08:20,960
+عليها بال بعد ربع ساعة طلعوا يشوفوا في الموبا اللي
+
+118
+00:08:20,960 --> 00:08:24,580
+حاطين عليها بال راحت الـ turbidity دلوقتي ليه؟ لأنّ
+
+119
+00:08:24,580 --> 00:08:30,360
+البكتيريا ماتت وتحلت وذابت وبيسموا الفحص bi
+
+120
+00:08:30,360 --> 00:08:32,180
+solubility test
+
+121
+00:08:37,720 --> 00:08:40,860
+هذا اللي هو الشائع اللي هو الصورة هذه هي مستعمرات
+
+122
+00:08:40,860 --> 00:08:45,140
+للبكتيريا على الطباط أضفنا قطرة من الـ bio على
+
+123
+00:08:45,140 --> 00:08:49,000
+الطباط اللي هو blood agar اختفت المستعمرات تماما
+
+124
+00:08:49,000 --> 00:08:56,500
+ذهبت مع ذلك الحكي أنّها bio soluble bio soluble
+
+125
+00:08:56,500 --> 00:09:00,820
+أذكركم بالـ in terracotta بالستربتوكوكاس فيريدانس
+
+126
+00:09:00,820 --> 00:09:06,770
+بما عنها أنها negative for all other tests هي
+
+127
+00:09:06,770 --> 00:09:10,990
+negative for bile, negative for bi-solubility هذا
+
+128
+00:09:10,990 --> 00:09:13,090
+الفحص بيميز من التنسيق، يعني ال spirit of coca
+
+129
+00:09:13,090 --> 00:09:16,510
+cinnamonia بيعيش bi-soluble بينما ال viridans
+
+130
+00:09:16,510 --> 00:09:21,670
+group non-bile أو non-soluble طب في كمان فحص كتير
+
+131
+00:09:21,670 --> 00:09:24,250
+مشهور، إذا ذكرت ال spirit of coca cinnamonia أيضا
+
+132
+00:09:24,250 --> 00:09:28,170
+يذكر، optochin disk وoptochin disk هو عبارة عن
+
+133
+00:09:28,170 --> 00:09:33,930
+إيه؟ عبارة عن antibiotic disk زي اللي استخدمناه في
+
+134
+00:09:33,930 --> 00:09:38,690
+الـ differentiation بين Staphylococcus epidermidis
+
+135
+00:09:38,690 --> 00:09:41,350
+و Staphylococcus saprophyticus اتعرف إيه اسمه كده
+
+136
+00:09:41,350 --> 00:09:47,230
+الفحص؟ إن هو بيوزع، تمام؟ هذا optochin بيميز بين
+
+137
+00:09:47,230 --> 00:09:50,770
+Staphylococcus pneumoniae و Staphylococcus viridans
+
+138
+00:09:50,770 --> 00:09:54,470
+ال pneumonia sensitive و لاحظوا ال zone of
+
+139
+00:09:54,470 --> 00:09:58,550
+inhibition اللي حوالين القرص واضح إن في DNA
+
+140
+00:09:58,550 --> 00:10:03,950
+killing للبكتيريا أول بكتيريا اللي هي Streptococcus 
+
+141
+00:10:03,950 --> 00:10:08,470
+pneumoniae ال sensitive ال viridans group فوق ما فيش
+
+142
+00:10:08,470 --> 00:10:11,990
+أي zone of inhibition لأن هي resistant فهي فحص
+
+143
+00:10:11,990 --> 00:10:16,930
+بميزة ال viridans عن مين؟ عن ال pneumoniae، bile-soluble
+
+144
+00:10:16,930 --> 00:10:22,170
+وoptochin resistance طبعا، بينما ال Streptococcus
+
+145
+00:10:22,170 --> 00:10:28,460
+pneumoniae bile-soluble وoptochin sensitiveتيجي بقى فحص
+
+146
+00:10:28,460 --> 00:10:32,340
+مشهور اسمه Quellung reaction أو له اسم تاني
+
+147
+00:10:32,340 --> 00:10:37,340
+capsular swelling test swelling إيش معنى swelling؟
+
+148
+00:10:37,340 --> 00:10:42,860
+swell انتفاخ ركزوا بقى هاي الصورة اللي أمامنا مش
+
+149
+00:10:42,860 --> 00:10:47,180
+معبرة عن ما أريد قوله شريحة بتاعة الشرايح
+
+150
+00:10:47,180 --> 00:10:52,220
+الميكروسكوبية العادية قطرة من ال saliva البكتيريا
+
+151
+00:10:52,220 --> 00:10:54,060
+المشتبه فيها mixing
+
+152
+00:10:58,190 --> 00:11:02,670
+تحت الميكروسكوب بنخفف الإضاءة قدر الإمكان ويعني لو
+
+153
+00:11:02,670 --> 00:11:06,010
+كان في ال dark field ميكروسكوبي لبأس في ذلك هنشوف
+
+154
+00:11:06,010 --> 00:11:10,850
+الـ diplococci و هنشوف الكابسول طبعا جنب ال cover
+
+155
+00:11:10,850 --> 00:11:14,250
+slip عارفين cover slip اللي فوق الشريحة بنضيف قطرة
+
+156
+00:11:14,250 --> 00:11:19,310
+من ال capsular antiserum إيش أنا antiserum يعني
+
+157
+00:11:19,310 --> 00:11:22,610
+antibodies ضد الكابسول بتاعت مين؟ تاعت ال Streptococcus
+
+158
+00:11:22,610 --> 00:11:27,100
+pneumoniae طبعا لازم يكون هذا المحلول بيحتوي
+
+159
+00:11:27,100 --> 00:11:33,120
+على 84 نوع من إيه؟ من ال antibodies لأن القطرة اللي
+
+160
+00:11:33,120 --> 00:11:37,120
+هتتحرك فيها بتمشي تحت ال cover slip بفعل إن
+
+161
+00:11:37,120 --> 00:11:40,280
+المسافة اللي بين ال cover slip و الشريحة مسافة
+
+162
+00:11:40,280 --> 00:11:43,960
+ضئيلة فخاصية شعرية بيصير فيه انتشار لمين؟ لل
+
+163
+00:11:43,960 --> 00:11:48,720
+antiserum إذا ال antibody ارتبط بال capsule أنا لن
+
+164
+00:11:48,720 --> 00:11:53,900
+أستطيع رؤية ال antibody أساسا كيف لي أن أعلم إنه
+
+165
+00:11:53,900 --> 00:11:56,840
+صار فيه ارتباط و لا ما صار فيه ارتباط عن طريق
+
+166
+00:11:56,840 --> 00:12:00,580
+انتفاخ الكابسولة و عشان هي سموها capsular swelling
+
+167
+00:12:00,580 --> 00:12:05,360
+test لو صار حجم الكابسولة بيزيد أكبر بضعفين أو ثلاث
+
+168
+00:12:05,360 --> 00:12:09,500
+أضعاف ال original اللي هو إيه؟ البكتيريا اللي كانت
+
+169
+00:12:09,500 --> 00:12:12,180
+موجودة هذا المؤشر على إن البكتيريا هي Streptococcus
+
+170
+00:12:12,180 --> 00:12:16,320
+pneumoniae كان مراقب اسمه Quellung reaction إذا نفحص
+
+171
+00:12:16,320 --> 00:12:19,360
+الآن هارفته مهمين لـ Streptococcus pneumoniae
+
+172
+00:12:30,190 --> 00:12:32,490
+هذا الفحص اللي قدامنا ليس له علاقة بالـ
+
+173
+00:12:32,490 --> 00:12:37,870
+Streptococcus pneumoniae لكن له علاقة بكل الـ
+
+174
+00:12:37,870 --> 00:12:41,770
+Streptococcus وذكركم بـ Rebecca Lancefield Rebecca
+
+175
+00:12:41,770 --> 00:12:46,150
+Lancefield قسمت البكتيريا لـ Streptococcus based on
+
+176
+00:12:46,150 --> 00:12:52,380
+surface antigen إلى groups A,B,C إلى آخرهم محصورة في
+
+177
+00:12:52,380 --> 00:13:00,220
+a و b و d الآن اللي بيحدث بضبط عندنا إن احنا عندنا
+
+178
+00:13:00,220 --> 00:13:05,720
+ست قنابيف ستة ضالة سبعة واحد اثنين ثلاثة أربعة خمسة
+
+179
+00:13:05,720 --> 00:13:09,020
+ستة والسبعة اللي اللي الأحمر دي control control إن
+
+180
+00:13:09,020 --> 00:13:12,940
+فيها بكتيريا بها بكتيريا الآن هدول عايش المكتوب
+
+181
+00:13:12,940 --> 00:13:17,880
+Anti-A وAnti-B وAnti-C وAnti-D وAnti-F وAnti-G هم
+
+182
+00:13:17,880 --> 00:13:20,240
+المراهم اللي احنا بقى نفسنا يبهمونا عزلنا
+
+183
+00:13:20,240 --> 00:13:23,200
+Streptococcus بنعرف اللي أي نوع بتنتمي هذا ال
+
+184
+00:13:23,200 --> 00:13:26,940
+Streptococcus فبناخدها و بنحطها على شريحة و
+
+185
+00:13:26,940 --> 00:13:30,540
+بندوبها في saline و بنضيف عليها قطرة من Anti-A و
+
+186
+00:13:30,540 --> 00:13:34,880
+قطرة من Anti-B على .. على .. أماكن مختلفة سبب تامة
+
+187
+00:13:34,880 --> 00:13:38,940
+هو Grouping Anti-A و Anti-B و Rh تمام؟ و بنعمل
+
+188
+00:13:38,940 --> 00:13:44,050
+Good Mixing إذا كانت البكتيريا من نوع C هتتفعل مع
+
+189
+00:13:44,050 --> 00:13:48,370
+مين؟ مع Anti-C وهيعمل إن هي إيه؟ agglutination طبعا
+
+190
+00:13:48,370 --> 00:13:54,050
+في كتات كل antigen .. آه أفضل كل antibody بلون
+
+191
+00:13:54,050 --> 00:13:57,650
+فبالتالي بيسهل علينا عملية اللي هو الـ .. إذا مش كل
+
+192
+00:13:57,650 --> 00:14:00,370
+antibody بلون هم مرتبين ترتيب alphabetically وعنا
+
+193
+00:14:00,370 --> 00:14:03,970
+شريحة هالمريكوا صورة عنها هي شريحة ثانية برضه
+
+194
+00:14:03,970 --> 00:14:06,590
+مرتبة alphabetically فاحنا ممكن نحط ال
+
+195
+00:14:06,590 --> 00:14:12,070
+corresponding antibody على الحرف Anti-A على دائرة A
+
+196
+00:14:12,070 --> 00:14:14,590
+على دائرة B و هكذا واللي بيعطينا positive
+
+197
+00:14:14,590 --> 00:14:18,270
+agglutination test clumping يعني زي اللي قلت اللي
+
+198
+00:14:18,270 --> 00:14:21,890
+كنا ال blood group طبعا لكن مش هتشوفوا visible
+
+199
+00:14:21,890 --> 00:14:26,390
+clumps بشكل واضح زي ال blood group لأن حجم
+
+200
+00:14:26,390 --> 00:14:30,770
+البكتيريا بقت أكبر من حجم ال blood cells هذا ال
+
+201
+00:14:30,770 --> 00:14:35,330
+Latex Agglutination هو فحص معتمد في المختبرات
+
+202
+00:14:35,330 --> 00:14:39,690
+للتعرف على antigens اللي هي ال Streptococcus
+
+203
+00:14:39,690 --> 00:14:42,990
+prevention and treatment of streptococcus
+
+204
+00:14:42,990 --> 00:14:49,290
+pneumoniae immunity 14 capsule types mixed vaccine
+
+205
+00:14:49,290 --> 00:14:53,850
+طبعا هنا كلمة prevention مهمة جدا وممكن أنا
+
+206
+00:14:53,850 --> 00:15:00,130
+هأقول لكم جاب إليكم إن ال vaccines أحدثت طفرة في منع
+
+207
+00:15:00,130 --> 00:15:03,530
+الأمراض المعدية اللي من ضمنها كان ال chickenpox و
+
+208
+00:15:03,530 --> 00:15:07,570
+ال smallpox و ال polio و ال rubella ال viral
+
+209
+00:15:07,570 --> 00:15:10,850
+diseases كتيرة ال mycobacterium tuberculosis كمان
+
+210
+00:15:10,850 --> 00:15:16,030
+اللي هي بال BCG فلما أنا بعطي فاكسين ضد ال
+
+211
+00:15:16,030 --> 00:15:20,190
+Streptococcus pneumoniae تذكروا أنها كانت أحد أسباب
+
+212
+00:15:20,190 --> 00:15:24,210
+أحد نجوم ال meningitis صح هذا لأ فبالتالي أنا بقلل
+
+213
+00:15:24,210 --> 00:15:29,470
+نسبة الإصابة بال meningitis most strains suitable
+
+214
+00:15:29,470 --> 00:15:32,550
+to penicillin but resistance is common هذه النقطة
+
+215
+00:15:32,550 --> 00:15:39,470
+أجنبيها في عندها شوية عشان نوضح زمان كان العلماء
+
+216
+00:15:39,470 --> 00:15:43,930
+يكتبوا مقالات ويقولوا يحبوا يفسروا لماذا ال
+
+217
+00:15:43,930 --> 00:15:47,610
+streptococcus ما عندهاش القدرة اللي موجودة عند
+
+218
+00:15:47,610 --> 00:15:52,090
+ال staphylococcus في ال resistance يعني بمعنى آخر
+
+219
+00:15:58,180 --> 00:16:00,560
+والدليل على أن الـ tonsillitis was caused by
+
+220
+00:16:00,560 --> 00:16:04,180
+Streptococcus pneumoniae لحد اليوم نسبة كبيرة ممكن
+
+221
+00:16:04,180 --> 00:16:07,080
+تنجح بعلاجها بالبنزلين العادي، بس هتقول
+
+222
+00:16:07,080 --> 00:16:09,580
+الـ بنزلين يعالج الـ staphylococcus which كان
+
+223
+00:16:09,580 --> 00:16:14,300
+تقريبا 99%، 95% resistant، بينما ال Streptococcus
+
+224
+00:16:14,300 --> 00:16:19,000
+لأ، فضلنا نقول ليش ال Streptococcus، طبعا صار في
+
+225
+00:16:19,000 --> 00:16:19,220
+نضايق
+
+226
+00:16:23,570 --> 00:16:26,070
+يعني مش شايفيني قدامكم غير ال Staphylococcus
+
+227
+00:16:26,070 --> 00:16:29,070
+aureus أو ال Staphylococci و أنا كمان عندي مفضلة
+
+228
+00:16:29,070 --> 00:16:34,210
+تبدأ تظهر سلالات مقاومة لمين؟ لل antibiotics لكن
+
+229
+00:16:34,210 --> 00:16:40,530
+بشكل عام حقيقة هي أقل resistance من مين؟ من لواء ال
+
+230
+00:16:40,530 --> 00:16:46,370
+Staphylococcus الميكروب الأخير في السلسلة اللي هو
+
+231
+00:16:46,370 --> 00:16:50,450
+الناسية نقوله نقدر نكمله اليوم عشان نبدأ موضوع
+
+232
+00:16:50,450 --> 00:16:51,730
+جديد في المرة القادمة
+
+233
+00:16:54,730 --> 00:16:58,570
+قبل ما ندخل على الموضوع الصورة اللي قدامنا مرعبة ل
+
+234
+00:16:58,570 --> 00:17:03,030
+Gram-negative diplococci Gram-negative diplococci
+
+235
+00:17:03,030 --> 00:17:06,710
+الموضوع اللي سبقها مباشرة كان Gram-positive
+
+236
+00:17:06,710 --> 00:17:09,970
+diplococci ده اللي بيخلطه من Grams 10 وبيعتبره من
+
+237
+00:17:09,970 --> 00:17:14,730
+Grams 10 مش يعني حاجة مش تقنية الفرق بين Gram
+
+238
+00:17:14,730 --> 00:17:17,250
+positive و Gram-negative إن هي Streptococcus
+
+239
+00:17:17,250 --> 00:17:20,210
+pneumoniae وها دي Neisseria gonorrhoeae أو Neisseria من هنا
+
+240
+00:17:20,210 --> 00:17:25,030
+ده فرق كبير فرق كبير في ال diagnosis لما أنا هتحشو
+
+241
+00:17:25,030 --> 00:17:28,450
+الـ camera شوية Neisseria meningitidis من نجوم ال meningitis
+
+242
+00:17:28,450 --> 00:17:32,650
+و Streptococcus pneumoniae من نجوم .. لما أنا أقول Gram
+
+243
+00:17:32,650 --> 00:17:35,510
+negative diplococci لما أقول Gram-positive diplococci
+
+244
+00:17:35,510 --> 00:17:41,830
+الـ Neisseria في إن اسمان مشهورين Neisseria
+
+245
+00:17:41,830 --> 00:17:46,310
+gonorrhoeae و Neisseria meningitidis هم الاسمين الأشهر اطلاقا
+
+246
+00:17:46,310 --> 00:17:49,470
+في هذا ال genus هذا لا يعني إنه ما فيش other Neisseria
+
+247
+00:17:51,240 --> 00:17:54,660
+ال females بشكل عام بيحتوي في ال vagina على normal
+
+248
+00:17:54,660 --> 00:17:57,040
+flora وهذه نقطة إننا نتذكرها كويس عشان لما نتكلم
+
+249
+00:17:57,040 --> 00:18:01,180
+عن ال diagnosis females carry Neisseria meningitidis
+
+250
+00:18:01,180 --> 00:18:05,920
+ولا gonorrhoeae، Neisseria sicca، Neisseria flava، Neisseria 
+
+251
+00:18:05,920 --> 00:18:10,240
+لاكتوميكا، أسماء ليس لها علاقة بالpathogenesis لكن
+
+252
+00:18:10,240 --> 00:18:14,740
+موجودة كـ normal flora في الفيميل فقط وبالتالي لها 
+
+253
+00:18:14,740 --> 00:18:16,520
+consequences في الـ diagnosis اللي هتكلم عنه كمان 
+
+254
+00:18:16,520 --> 00:18:16,880
+شوية
+
+255
+00:18:20,010 --> 00:18:23,470
+Diplokokai, pairs of cocci, oxidase, bostrim ال
+
+256
+00:18:23,470 --> 00:18:27,730
+pairs هدولة بتعرفوا حبات القهوة؟ لما نواجه حبة 
+
+257
+00:18:27,730 --> 00:18:33,070
+قهوة مش منحني هي زي حرف الـ D صح؟ حب الـ D .. حرف الـ
+
+258
+00:18:33,070 --> 00:18:36,490
+D و حرف الـ D، حب القهوة و حب القهوة، على جبال بعض أو
+
+259
+00:18:36,490 --> 00:18:40,710
+حبة فصولي يعني، حبة فصولي، بسموها kidney .. kidney
+
+260
+00:18:41,900 --> 00:18:46,340
+شيب أو kidney bean shape يعني أو اللي قولنا الـ D
+
+261
+00:18:46,340 --> 00:18:49,980
+اللي flattened at the adjacent sites يعني المناطق
+
+262
+00:18:49,980 --> 00:18:54,960
+المتسقة من الحرفين الـ D بكونوا إيه؟ flat إيش يعني
+
+263
+00:18:54,960 --> 00:19:00,620
+flat؟ يعني مسطحين مش دائرين طيب البكتيريا بتنمو 
+
+264
+00:19:00,620 --> 00:19:03,100
+على الـ blood agar اه بتنمو على الـ blood agar لكن
+
+265
+00:19:03,100 --> 00:19:07,230
+احنا بنميها غالبا على الـ chocolate agar و نجف عند
+
+266
+00:19:07,230 --> 00:19:09,930
+كلمة chocolate agar بتتكلم عنه شوية عشان احنا
+
+267
+00:19:09,930 --> 00:19:14,150
+نوضّح بعض الأمور المهمة جدا في هذا المجال ال
+
+268
+00:19:14,150 --> 00:19:17,070
+chocolate agar ليس له علاقة بالchocolate هو بالـ
+
+269
+00:19:17,070 --> 00:19:21,350
+blood تم تعريضه لحرارة فتغير لونه لوسط الـ chocolate
+
+270
+00:19:21,350 --> 00:19:26,110
+تمام الآن هو blood agar تم تسخينه على 80 درجة
+
+271
+00:19:26,110 --> 00:19:30,570
+مئوية فتحول اللون من اللون الأحمر المعروف إلى
+
+272
+00:19:30,570 --> 00:19:33,450
+اللون البني اللي بيشبه الـ chocolate اللي بتسميها
+
+273
+00:19:33,450 --> 00:19:38,970
+جاء من اللون فقط لهطب السؤال اللي بيطرح نفسه blood
+
+274
+00:19:38,970 --> 00:19:43,430
+agar و chocolate agar هما نفس التركيبة الفرق في
+
+275
+00:19:43,430 --> 00:19:47,070
+التحضير واحد حضرنا على 80 درجة مئوية الـ blood و
+
+276
+00:19:47,070 --> 00:19:50,810
+الثاني على 50 إلى 55 درجة مئوية what is the
+
+277
+00:19:50,810 --> 00:19:53,390
+difference between the two؟ ليش هلأ بيستخدموا
+
+278
+00:19:53,390 --> 00:19:57,030
+أغراضها .. بيستخدموا أغراضها؟ بفضل المفروض أن 
+
+279
+00:19:57,030 --> 00:20:01,170
+خلايا الدم بيكون فيها factor دخلتها لما .. لما 
+
+280
+00:20:01,170 --> 00:20:04,350
+اتعرضت لليه طلعت لبرا اللي ماكانش قبل يستفيد منها
+
+281
+00:20:04,350 --> 00:20:05,730
+لما أن اتصلت
+
+282
+00:20:12,880 --> 00:20:15,680
+أنا بتوقع أن الـ blood اللي خدناه .. خدناه من إنسان
+
+283
+00:20:15,680 --> 00:20:17,720
+أو خدناه من حيوان تبتعد تسمع عن sheep blood أو
+
+284
+00:20:17,720 --> 00:20:19,980
+horse blood أو حتى احنا في غزة غالبا بيستخدم الـ
+
+285
+00:20:19,980 --> 00:20:24,360
+human blood الـ human blood هو المدافر في الـ ..
+
+286
+00:20:24,360 --> 00:20:29,360
+للمحتوى فقط اللي بيستخدموه سواء كان sheep أو horse
+
+287
+00:20:29,360 --> 00:20:33,160
+أو قولنا مين human blood نتوقع أن يكون فيه مواد 
+
+288
+00:20:33,160 --> 00:20:38,140
+سامة للبكتيريات و توكسيك الدم هو فيه مواد و فيه 
+
+289
+00:20:38,140 --> 00:20:41,820
+antibiotics و فيه complement صح ولا التسخين غالبا
+
+290
+00:20:41,820 --> 00:20:47,020
+بيعمل destruction لهذه المواد يعني بمعنى آخر بيقلل
+
+291
+00:20:47,020 --> 00:20:52,160
+من تأثير الدم السام أو الضار أو الملّح النمو تمام؟
+
+292
+00:20:52,160 --> 00:20:56,680
+بيجي تسخين كويس كمان لكن في المقابل التسخين أيضا
+
+293
+00:20:56,680 --> 00:20:58,040
+كما بتعرفوا أنه
+
+294
+00:21:01,100 --> 00:21:04,260
+بيعمل destruction ليش بس للمواد الضارة، قد يقوم 
+
+295
+00:21:04,260 --> 00:21:12,160
+بعمل destruction لمواد غذائية مطلوبة لنمو 
+
+296
+00:21:12,160 --> 00:21:12,780
+البكتيريا
+
+297
+00:21:20,030 --> 00:21:22,390
+بيحتاجوا على نفسي المكونات تبع الـ blood agar مع
+
+298
+00:21:22,390 --> 00:21:28,510
+اختلافات طفيفة اللي سببها اللي هو التسخين بالحرارة 
+
+299
+00:21:28,510 --> 00:21:31,350
+صار عندي بعض المواد متاحة في الوسط ماكانتش متاحة
+
+300
+00:21:31,350 --> 00:21:36,170
+صار عندي مواد صار لها inhibition رميرات وصار في عندي
+
+301
+00:21:36,170 --> 00:21:40,690
+مواد أيضا خسرناها مواد غذائية الآن السؤال اللي 
+
+302
+00:21:40,690 --> 00:21:44,190
+بيطرح نفسه كمان مرة مين أحسن الـ blood agar ولا الـ
+
+303
+00:21:44,190 --> 00:21:49,380
+chocolate agar حسب البلد اللي ممكن عشان بتحتاج في
+
+304
+00:21:49,380 --> 00:21:52,440
+ناس بدأت تتعلم و تقول أنه ما فيش إمكانية على إجاب
+
+305
+00:21:52,440 --> 00:21:56,080
+هذا السؤال بأبيض و أسود، إن هو أحسن من هذا، في
+
+306
+00:21:56,080 --> 00:22:00,840
+عندنا استخدام و استخدام آخر، بمعنى أن الـ
+
+307
+00:22:00,840 --> 00:22:03,000
+chocolate agar له استخدامات و الـ butter agar له 
+
+308
+00:22:03,000 --> 00:22:07,900
+استخدامات أخرى، ليش ذكرنا الـ chocolate agar في
+
+309
+00:22:07,900 --> 00:22:12,340
+النيسيريا؟ النيسيريا جنورية بالذات is very fragile
+
+310
+00:22:12,340 --> 00:22:17,590
+organism، إيش يعني fragile؟ إيش يعني هشة؟ يعني أي
+
+311
+00:22:17,590 --> 00:22:23,290
+شيء بيأثر عليها ممكن تموت إيش يعني أي شيء؟ عارفين 
+
+312
+00:22:23,290 --> 00:22:27,490
+درجة حرارة 38 ممكن تقتلها؟ درجة حرارة 38 ممكن 
+
+313
+00:22:27,490 --> 00:22:33,930
+تقتلها لو حطينا كحول على مكان الإصابة، تمام؟ و ..
+
+314
+00:22:33,930 --> 00:22:37,510
+و ده الجزء الفاسد جدا من الكحول، ممكن تموت لو
+
+315
+00:22:37,510 --> 00:22:39,610
+حطيناها فوق تلك الكحولة، بعد ما نخلص من شغل
+
+316
+00:22:39,610 --> 00:22:42,090
+البكتيريا، و إيه بحطها؟ فوق الـ incubator، جنب الـ
+
+317
+00:22:42,090 --> 00:22:45,400
+incubator في علبة، لعلينا نتخلص من الـ autoclave لو
+
+318
+00:22:45,400 --> 00:22:48,740
+قعدت يوم فوق الـ incubator أو يومين بتموت بينما الـ
+
+319
+00:22:48,740 --> 00:22:50,840
+E.coli لو بعد أسبوعين لاجيناها بيبقى بيبقى بيبقى
+
+320
+00:22:50,840 --> 00:22:52,060
+بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى
+
+321
+00:22:52,060 --> 00:22:52,460
+بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى
+
+322
+00:22:52,460 --> 00:22:52,920
+بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى
+
+323
+00:22:52,920 --> 00:23:00,460
+بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى
+
+324
+00:23:00,460 --> 00:23:12,200
+بيبقى بيبقى
+
+325
+00:23:12,200 --> 00:23:18,020
+بيأخذنا في بعض البكتيريا ومنها النيسيريا جونورية
+
+326
+00:23:18,020 --> 00:23:24,100
+بتحتاج CO2 فكيف نوفرها لها؟ بنجيب إناء مغلق
+
+327
+00:23:24,100 --> 00:23:27,680
+محكم الغلق بنفتحه بنحط البكتيريا اللي الأطباق اللي
+
+328
+00:23:27,680 --> 00:23:31,560
+بنزرعها أو زرعناها و بنجيب شريحة و بنلزق عليها
+
+329
+00:23:31,560 --> 00:23:35,300
+شمعة و بنولع الشمعة و بننزلها في الـ .. في الإناء
+
+330
+00:23:35,300 --> 00:23:39,320
+و بنسكن الآن الشمعة هتستهلك معظم الـ .. أو ما نيش
+
+331
+00:23:39,320 --> 00:23:42,520
+أقول معظم كمية كبيرة من الـ oxygen و بعدين تنطفى
+
+332
+00:23:42,520 --> 00:23:46,850
+تستهلك الـ oxygen أو جزء من الأكسجين و بتطلب منها
+
+333
+00:23:46,850 --> 00:23:51,330
+CO2 فبتوفر أنهم يسموها CO2 jar تقنية مشهورة هتعلمها 
+
+334
+00:23:51,330 --> 00:23:57,190
+في المختبر إن شاء الله واحد طلاب خلص الشمع فاخذنا
+
+335
+00:23:57,190 --> 00:24:00,910
+شمع ثاني من نوع ثاني قعدنا أربع خمس أيام ولا
+
+336
+00:24:00,910 --> 00:24:04,110
+عزلنا نيسيريا جنورية كنا نازلينها في اليوم ست سبع
+
+337
+00:24:04,110 --> 00:24:08,190
+مرات ناست سبع أيام يعني نازلينها في الفترة اللي 
+
+338
+00:24:08,190 --> 00:24:13,630
+غيرنا فيها الشمع zero isolates negative تلافينا أن
+
+339
+00:24:13,630 --> 00:24:16,910
+الشمع اللي استخدمناه هو شمع أحمر و الشمع الأحمر
+
+340
+00:24:16,910 --> 00:24:20,630
+هو عبارة عن صبغة و الصبغة fumes بتطلع مع الحرق
+
+341
+00:24:20,630 --> 00:24:25,330
+تمام فمن كثر ما البكتيريا حساسة لم تنمو ولا واحدة
+
+342
+00:24:25,330 --> 00:24:29,610
+عملها دلوقتي فإن نيسيريا جونوريا is very fragile
+
+343
+00:24:29,610 --> 00:24:33,870
+very sensitive فبتتعامدها و الوسط يكون خالي من الـ
+
+344
+00:24:33,870 --> 00:24:37,130
+inhibitors في وقت بتاع اسمه مضطر مارتن أجار هو 
+
+345
+00:24:37,130 --> 00:24:42,110
+selective media يشبه في تركيبه إلى حد كبير الـ
+
+346
+00:24:42,110 --> 00:24:44,950
+chocolate agar لكن يضاف إليها ثلاث أنواع من الـ
+
+347
+00:24:44,950 --> 00:24:50,170
+antibiotics لازم أكتب لكم إياهم عشان نعرف الـ
+
+348
+00:24:50,170 --> 00:24:55,470
+antibiotics بيختصرونهم هو بيختصر بواسطة Tm اللي 
+
+349
+00:24:55,470 --> 00:25:01,730
+هو ثانية المارة نجار Vcn الـ Vcn هدولها ثلاث أحرف
+
+350
+00:25:01,730 --> 00:25:06,530
+هم ثلاث أسماء لثلاث انتبايتك Vcn
+
+351
+00:25:20,280 --> 00:25:28,340
+vancomycin كولستين نستاتين
+
+352
+00:25:28,340 --> 00:25:32,100
+كتبرهم ولا واضحين؟
+
+353
+00:25:56,790 --> 00:26:02,610
+Vancomycin كولستين ونستاتين طبعا الـ Vancomycin
+
+354
+00:26:02,610 --> 00:26:08,850
+نستخدمه لمنع الجرام positive بكتيريا الكولستين
+
+355
+00:26:08,850 --> 00:26:15,930
+نستخدمه لمنع الجرام negative بكتيريا النستاتين
+
+356
+00:26:15,930 --> 00:26:18,010
+نستخدمه لمنع الفطريات
+
+357
+00:26:41,590 --> 00:26:46,430
+طبعا هنا بيكونوا يعرفوا أن الـ genital tract
+
+358
+00:26:46,430 --> 00:26:52,090
+infection و الـ urinary بيسميه genitourinary tract
+
+359
+00:26:52,090 --> 00:26:57,880
+يعني في اتصال بينهم، فلو المرض عنده Infection بجرام 
+
+360
+00:26:57,880 --> 00:27:01,040
+نيجاتف زي الاشريشيا كولاي، هتعمل over grow على
+
+361
+00:27:01,040 --> 00:27:05,830
+الناسيريا، لو عندها enterococcus، المريض عنده
+
+362
+00:27:05,830 --> 00:27:09,110
+enterococcus، هتعمل overgrow على النيسيريا، لو عند
+
+363
+00:27:09,110 --> 00:27:11,930
+المريض Infection بيكانديا albicans، برضه هتعمل
+
+364
+00:27:11,930 --> 00:27:16,570
+overgrow على اللي هو الـ .. الـ .. الـ .. الـ ..
+
+365
+00:27:16,570 --> 00:27:19,130
+النيسيريا، وبالتالي احنا حاطينا الـ Antibiotics
+
+366
+00:27:19,130 --> 00:27:24,050
+تقوله على أساس أن نمنع نمو هذه البكتيريا،
+
+367
+00:27:24,050 --> 00:27:28,190
+وبالتالي نسمح للنيسيريا gonorrhea، لأن كمان مرة هي
+
+368
+00:27:28,190 --> 00:27:32,070
+حساسة وما عندناش إمكانية للمنافسة مع بكتيريا أخرى
+
+369
+00:27:44,710 --> 00:27:47,450
+هو المشكلة أن هذا الميكروب ممكن تموت لو كان في
+
+370
+00:27:47,450 --> 00:27:50,510
+أماكن بيصل فيها هذه الحرارة كل ما عن الـ genitorial
+
+371
+00:27:50,510 --> 00:27:53,610
+tract غالب الـ external genitalia ده الـ male و الـ
+
+372
+00:27:53,610 --> 00:27:56,570
+female درجة حرارتها بيكون أقل من درجة حرارة الجسم 
+
+373
+00:27:56,570 --> 00:27:59,810
+فحتى لو ارتفعت سبعة و ثلاثين و تسعة و ثلاثين و
+
+374
+00:27:59,810 --> 00:28:01,750
+تسعة و ثلاثين و تسعة و ثلاثين ممكن ما تؤثرش على الـ
+
+375
+00:28:01,750 --> 00:28:02,710
+.. الـ .. الـ .. الـ .. الـ 
+
+376
+00:28:05,730 --> 00:28:08,710
+بس للتمييز بين نيسيريا غونوريا و نيسيريا ميننغيتس
+
+377
+00:28:08,710 --> 00:28:11,990
+في عندنا بعض ال virulence factors they have
+
+378
+00:28:11,990 --> 00:28:15,690
+similar virulence factors شوفوا ال نيسيريا
+
+379
+00:28:15,690 --> 00:28:18,190
+ميننغيتس احنا مركزينه على ال ليبوبوليساكرايد
+
+380
+00:28:18,190 --> 00:28:21,670
+مضخمينه ال ليبوبوليساكرايد و ال capsule و لأن
+
+381
+00:28:21,670 --> 00:28:26,830
+هذولا هم أهم عاملين للبكتيريا لنيسيريا ميننغيتس
+
+382
+00:28:26,830 --> 00:28:30,230
+تمام اللي بيعمله infection و بيقال لها تعمل
+
+383
+00:28:30,230 --> 00:28:34,370
+meningitis لكن عندها برضه مجموعة من العوامل زي
+
+384
+00:28:34,370 --> 00:28:34,870
+اللي هموليسين
+
+385
+00:28:39,920 --> 00:28:42,300
+هتعرف على إيش هتنقل بس بروتين و أكثر من برو
+
+386
+00:28:42,300 --> 00:28:45,660
+تين كمان شوية على جهة تانية المقابلة أبرزنا
+
+387
+00:28:45,660 --> 00:28:49,760
+مين؟ أبرزنا ال بالاي أكثر حاجة بس أنا .. أنا ..
+
+388
+00:28:49,760 --> 00:28:53,020
+أنا لخاطر أكمل كمان ال IgA Protease IgA Protease
+
+389
+00:28:53,020 --> 00:28:56,700
+كتير مهم في ده الموضوع بس ال بالاي بالفعل هي
+
+390
+00:28:56,700 --> 00:29:01,240
+اللي لها ما عندهاش capsule ما عندهاش hemolysin بينما
+
+391
+00:29:01,240 --> 00:29:03,380
+ان نيسيريا ميننغيتس عندها capsule
+
+392
+00:29:06,920 --> 00:29:10,100
+نيسيريا غونوريا و بندللها بالاسم الشائع عليها
+
+393
+00:29:10,100 --> 00:29:14,120
+Gonococcus نيسيريا ال mainly طيبة اسم اللي
+
+394
+00:29:14,120 --> 00:29:17,680
+بندللها هو هنا ميننغوكوكس الستريبتوكوكس
+
+395
+00:29:17,680 --> 00:29:23,620
+نيومونيا هي تلاتة صارو كوكس ميننغوكوكس و غونوكوكس و
+
+396
+00:29:23,620 --> 00:29:27,560
+نيوموكوكس أخذنا الشق الأول منه ال species و
+
+397
+00:29:27,560 --> 00:29:31,960
+أعطينا كلمة كوكس ميننغوكوكس نيوموكوكس و غونو
+
+398
+00:29:31,960 --> 00:29:35,620
+كوكس ال incubation period من اثنين لأربعة أيام
+
+399
+00:29:36,240 --> 00:29:39,180
+فهو بيقول لي إن مانو مانو مقصود فيها إنسان
+
+400
+00:29:39,180 --> 00:29:43,500
+ليس مقصود فيها الرجل وإنما الإنسان هي بتصيب البظر
+
+401
+00:29:43,500 --> 00:29:50,200
+و الإحليل الجملة جملة كثير مهمة is the second most
+
+402
+00:29:50,200 --> 00:29:53,400
+common venereal disease كلمة venereal STD sexually
+
+403
+00:29:53,400 --> 00:29:56,480
+transmitted disease sexually transmitted disease
+
+404
+00:29:56,480 --> 00:30:00,600
+كلمة 
+
+405
+00:30:00,600 --> 00:30:07,070
+second most common يعني تتلو أو تتبع ال number one
+
+406
+00:30:07,070 --> 00:30:11,090
+من ال number one اللي هي ال chlamydia ال chlamydia
+
+407
+00:30:11,090 --> 00:30:16,510
+مشهورة بتسبب إسهال بشكل كبير تلعب الصورة اللي
+
+408
+00:30:16,510 --> 00:30:19,950
+قدامكم هذه الصورة اللي قدامنا هنا مأخوذة واحدة
+
+409
+00:30:19,950 --> 00:30:24,310
+منهم من ال pure culture ومكبرة أكثر منها تلعب دبلة
+
+410
+00:30:24,310 --> 00:30:30,950
+كوكاي واحد اثنين واحد اثنين واحد اثنين وفي في
+
+411
+00:30:30,950 --> 00:30:36,400
+الصورة الثانية إذا بتلاحظوا في عندي نيوترفيل،
+
+412
+00:30:36,400 --> 00:30:40,560
+النيوتروفيل فيها نيوكلياس و مليانة دبلوكوكاي،
+
+413
+00:30:40,560 --> 00:30:47,480
+مليانة دبلوكوكاي، هي واحدة كل هذه و هذه لكن كمان
+
+414
+00:30:47,480 --> 00:30:52,960
+برضه بلاقي دبلوكوكاي الموجودة في المحيط فبنجي
+
+415
+00:30:52,960 --> 00:30:56,640
+نقول عنها إنه exists intracellular and
+
+416
+00:30:56,640 --> 00:30:58,160
+extracellular
+
+417
+00:31:00,450 --> 00:31:04,310
+it can exist, it can grow freely وده يعني ده جانب
+
+418
+00:31:04,310 --> 00:31:07,890
+اللي بنميها في المختبر بطريقة ال ... ال plates هذا
+
+419
+00:31:07,890 --> 00:31:11,050
+المعيشة عنها بتنمو إيه؟ freely، لو إنها obligate
+
+420
+00:31:11,050 --> 00:31:13,610
+intracellular بنات ما بنجرش نميها إلا على cell
+
+421
+00:31:13,610 --> 00:31:18,450
+culture بس لما بيقول facultative intracellular
+
+422
+00:31:18,450 --> 00:31:24,380
+بتجي تنمو، هانو تنمو على داخل الخلايا المصطلح هو
+
+423
+00:31:24,380 --> 00:31:28,320
+polymorphonuclear cells إيه كانت polymorphonuclear
+
+424
+00:31:28,320 --> 00:31:31,880
+cells؟ و بيختصروها PMNS PMNS
+
+425
+00:31:31,880 --> 00:31:37,040
+polymorphonuclear cells هي نفسها بقية النيوترفيل
+
+426
+00:31:37,040 --> 00:31:40,740
+هي نفسها الفاغوسايتز يعني اللي بتكلم على PMNS
+
+427
+00:31:40,740 --> 00:31:44,440
+أو بتكلم عن نيوترفيل أو فاغوسايتز بتكلم عن نفس
+
+428
+00:31:44,440 --> 00:31:48,440
+الخط نفس
+
+429
+00:31:48,440 --> 00:31:53,060
+الصورة طيب نيجي ل ال ... ال pilli و ال proteins
+
+430
+00:31:53,060 --> 00:31:58,040
+pilli is important in adhering أعتقد إنه حكيناها
+
+431
+00:31:58,040 --> 00:32:00,740
+أوكي لما ... لما كنا نتكلم عن ال variants factor في
+
+432
+00:32:00,740 --> 00:32:05,220
+كل عام لأ ذكرت أنا إن النقطة كثير مهمة إنه ال
+
+433
+00:32:05,220 --> 00:32:09,340
+pilli أهميتها بالنسبة للجنودية تختلف عن كل ... و
+
+434
+00:32:09,340 --> 00:32:12,380
+لذلك أبرزناها احنا بخط كبير في اللوحة لما عملنا
+
+435
+00:32:12,380 --> 00:32:18,130
+مقارنة بين التانية ما هي ميزتها؟ إن الرسم تحرف
+
+436
+00:32:18,130 --> 00:32:22,070
+أهمية البيلاي فكوّن عيساها مضادة، هي عرفت إن الرسم
+
+437
+00:32:22,070 --> 00:32:28,010
+فصارت عاملة genetic variation أو عفوا gene
+
+438
+00:32:28,010 --> 00:32:31,570
+variation وبالتالي صارت تدخلنا برضه مرارا وتكرارا
+
+439
+00:32:31,570 --> 00:32:37,410
+بأشكال متنوعة من ال antigen تبع البيلاي تخيلوا كده
+
+440
+00:32:37,410 --> 00:32:43,470
+أهميتها non-pileated strains أو mutants are aviral
+
+441
+00:32:45,010 --> 00:32:47,210
+اللي ما لهاش كابسول تبعت لستريبتوكوكا نيومونيا اللي
+
+442
+00:32:47,210 --> 00:32:52,170
+ما لهاش كابسول ما لهاش pathogenesis فاللي ما لهاش
+
+443
+00:32:52,170 --> 00:32:57,170
+بيلايهانا ما لهاش أي pathogenesis من الأوتر
+
+444
+00:32:57,170 --> 00:33:00,670
+proteins في عندنا protein واحد و protein اثنين و
+
+445
+00:33:00,670 --> 00:33:04,270
+protein تلاتة لكن كل واحد منهم له اسم آخر غير عن
+
+446
+00:33:04,270 --> 00:33:09,050
+الرقم المعطى فمثلا part of protein اللي بيسموه
+
+447
+00:33:09,050 --> 00:33:12,250
+protein واحد prevent phagolizosome fusion أظن إن
+
+448
+00:33:12,250 --> 00:33:16,900
+كلكم فاكرين الفاجوزوم الفاجوزوم اللي جواته
+
+449
+00:33:16,900 --> 00:33:22,200
+البكتيريا تمام؟ و الليسوزوم اللي بيجي بيتحد معه
+
+450
+00:33:22,200 --> 00:33:26,880
+الآن العملية الضرورية في ال normal phagocytosis
+
+451
+00:33:26,880 --> 00:33:31,040
+حددت ال porn protein بيمنع الالتحام ما بين
+
+452
+00:33:31,040 --> 00:33:37,000
+الفاجوزوم والليزوزوم بيكونش فاجوليزوزوم وبالتالي
+
+453
+00:33:37,000 --> 00:33:40,560
+بيبقى وانا اللي بيخلي البكتيريا قادرة على إنها
+
+454
+00:33:40,560 --> 00:33:41,940
+تعمل intracellular
+
+455
+00:33:44,120 --> 00:33:49,140
+وبست بروتين وهذا بيساعد البكتيريا بشكل جيد جدا على
+
+456
+00:33:49,140 --> 00:33:52,240
+ال binding يعني هو أبدا عن attachment أبدا عن
+
+457
+00:33:52,240 --> 00:33:56,180
+attachment في كمان هاسمها RMB أو reduction
+
+458
+00:33:56,180 --> 00:33:59,480
+modifiable protein وهذا بيعمل عملية protection
+
+459
+00:33:59,480 --> 00:34:04,500
+للبكتيريا من ال bactericidal antibodies إذا على
+
+460
+00:34:04,500 --> 00:34:10,500
+الرغم من إنها حساسة إلا إنها مزودة يعني بعبارة أخرى هي
+
+461
+00:34:10,500 --> 00:34:18,070
+مصممة designed ولما قلت only for man موجودة فقط في
+
+462
+00:34:18,070 --> 00:34:22,830
+الإنسان ولا تنتقل بطرق أخرى إلا عبر الجنس إذا لمن
+
+463
+00:34:22,830 --> 00:34:27,350
+يمارس هنا الجماع هي مخصصة ومصممة لهم أنت هنا يعني
+
+464
+00:34:27,350 --> 00:34:33,290
+حتى يعني طبعا إمكانية الهياجت موجودة لكن لاحظته في
+
+465
+00:34:33,290 --> 00:34:39,990
+ال immunity إنه انعطمت عضنا لأنه في بإمكانية تغيير
+
+466
+00:34:39,990 --> 00:34:46,380
+للي هو ال بالعيب قدرتها على مكافحة ال ... ال ...
+
+467
+00:34:46,380 --> 00:34:50,420
+ال ... ال antibiotics عالي جدا، يعني أنا أذكر في ...
+
+468
+00:34:50,420 --> 00:34:56,210
+يمكن قبل عشرين سنة قرأت مقالة عن Hong Kong Hong
+
+469
+00:34:56,210 --> 00:35:00,730
+Kong ونسبة ال resistance لل نيسيريا الجنودية في
+
+470
+00:35:00,730 --> 00:35:05,150
+Hong Kong كانت النسبة أكثر من 95% resistant لل
+
+471
+00:35:05,150 --> 00:35:07,470
+Penicillin اللي كان drug of choice كان drug of
+
+472
+00:35:07,470 --> 00:35:10,410
+choice في يوم من الأيام وبالتالي صحيح هي delicate
+
+473
+00:35:10,410 --> 00:35:15,970
+outside حتى لا تنتقل للناس العاديين تمام؟ اللي
+
+474
+00:35:15,970 --> 00:35:20,250
+ما لهمش دعوة وبالتالي تقولنا كده مرة واضح إنها
+
+475
+00:35:20,250 --> 00:35:23,390
+specifically designed يعني مصممة لهذا الغرض
+
+476
+00:35:26,420 --> 00:35:28,840
+طبعا أنا لم ... أنا لم ... أنا لم ... يعني أجروا إن
+
+477
+00:35:28,840 --> 00:35:32,040
+أحط لكم صور لنيسيريا الغونوريا لأن كلها بقعد
+
+478
+00:35:32,040 --> 00:35:35,180
+external genitalia كلها و العرض الوحيد اللي هو
+
+479
+00:35:35,180 --> 00:35:41,040
+عملية ال discharge اللي هي الإفرازات الصدرية اللي
+
+480
+00:35:41,040 --> 00:35:44,520
+بتخرج من ال ... ال female organ أو ال male organ
+
+481
+00:35:44,520 --> 00:35:50,640
+لكن دي بتصورة بتوضح الضحية اللي بيولد لأم مصابة ب
+
+482
+00:35:50,640 --> 00:35:55,010
+... اللي هو ال ... ال ... الغونوريا خلال ... أظن إننا
+
+483
+00:35:55,010 --> 00:35:59,310
+حكينا عن قناة الولادة إن الطفل بياخد معه ميكروبات
+
+484
+00:35:59,310 --> 00:36:03,330
+وهذه الميكروبات الأصل إنها كويسة ... كويسة أقول لكم
+
+485
+00:36:03,330 --> 00:36:07,190
+بدونها ... بدونها يعني الطفل ... فاكريني لما مرة
+
+486
+00:36:07,190 --> 00:36:09,890
+ناقشنا قولنا إنه الأطفال اللي عندهم ... بناخدهم
+
+487
+00:36:09,890 --> 00:36:13,330
+قيصرية أكثر عرضة ممكن تكون السبب إنهم ما ياخدوش
+
+488
+00:36:13,330 --> 00:36:16,150
+عبر قناة الولادة وما ياخدوش منه ال M normal
+
+489
+00:36:16,150 --> 00:36:19,910
+microbiota لكن لو يكون فيه pathogens بعض الطفل
+
+490
+00:36:19,910 --> 00:36:23,930
+بياخد pathogen وفي هذه الحالة هو جهاز المناعة لم
+
+491
+00:36:23,930 --> 00:36:27,670
+يكتمل بعد إنه ال flora تبعه لم تكتمل بعد وبالتالي
+
+492
+00:36:27,670 --> 00:36:30,710
+قد يصاب بإلتهابات في العين بنسميها ophthalmia
+
+493
+00:36:30,710 --> 00:36:34,450
+neonatrum ولاحظوا الصديد اللي طالع من العين كميات
+
+494
+00:36:34,450 --> 00:36:39,250
+بكمية من الصديد نفس لون الصديد هذا هو اللي بنشوفه
+
+495
+00:36:39,250 --> 00:36:44,690
+خارج من ال male أو ال female genital area أو organ
+
+496
+00:36:44,690 --> 00:36:48,930
+طبعا ال ophthalmia neonatrum condition ما بتسببهوش
+
+497
+00:36:48,930 --> 00:36:52,420
+بكتيريا واحدة بسبب مجموعة من ال micro organism من
+
+498
+00:36:52,420 --> 00:36:55,280
+ضمنها اللي هي ... ما أعرف شو هي الواضحة دي لأ صبورة
+
+499
+00:36:55,280 --> 00:36:58,200
+اللي هي ال clamydia اللي هي جناها نشوية number
+
+500
+00:36:58,200 --> 00:37:05,460
+one cause of asterese أو venereal disease ال 
+
+501
+00:37:05,460 --> 00:37:08,920
+Gram stain it is poly... it can be found in
+
+502
+00:37:08,920 --> 00:37:11,240
+polymorph vascular cells شفتوا نجو شوية في ال
+
+503
+00:37:11,240 --> 00:37:14,750
+neutrophil صح؟ Gram negative cocci either
+
+504
+00:37:14,750 --> 00:37:17,910
+intracellular or extracellular either يعني ممكن
+
+505
+00:37:17,910 --> 00:37:21,310
+نجيها الشريحة الواحدة اللي جيها في التانين اللي
+
+506
+00:37:21,310 --> 00:37:25,810
+جيها intracellular و extracellular الآن هنا النقطة
+
+507
+00:37:25,810 --> 00:37:29,050
+أذكركم الآن أخذنا Grams 10 من أنثى و أخذنا Grams
+
+508
+00:37:29,050 --> 00:37:32,790
+10 من ذكر اختلف الوضع صح؟ إذا ولدنا Gram negative
+
+509
+00:37:32,790 --> 00:37:35,930
+دبله كوكاية في الذكر لأن احنا بنعرف إنه ما عندهوش 
+
+510
+00:37:35,930 --> 00:37:38,590
+Neisseria normal flora فعنده Neisseria gonorrhoeae
+
+511
+00:37:38,590 --> 00:37:41,450
+شخصناهالكم لو أخدناها من أنثى ولا جانا عندها
+
+512
+00:37:41,450 --> 00:37:44,930
+جرامية تدبل الكوكاية، يمكنها تكون normal flora،
+
+513
+00:37:44,930 --> 00:37:49,890
+لذلك أصلا في المختبرات يرفض استقبال urethral
+
+514
+00:37:49,890 --> 00:37:53,990
+discharge كجراميستين لـ Neisseria gonorrhoeae لما تكون عينة
+
+515
+00:37:53,990 --> 00:37:59,180
+من 100 من أنثى، لأنها useless فقط في الـ culture
+
+516
+00:37:59,180 --> 00:38:02,980
+طبعا لأ أصلا يعني مقتنعش على الـ Gram stain في
+
+517
+00:38:02,980 --> 00:38:06,480
+التشخيص النهائي ولكن كتشخيص مبدأي إيه؟ في الـ Dakar
+
+518
+00:38:06,480 --> 00:38:09,020
+ممكن نقول إنه عندنا Neisseria gonorrhoeae لكن في الوقت
+
+519
+00:38:09,020 --> 00:38:11,980
+لأ ما أستطيع إلا على طريق اللي هو وطريقة اللي هو
+
+520
+00:38:11,980 --> 00:38:14,920
+أصلا في الآخر culture على طير Martin agar أنت
+
+521
+00:38:14,920 --> 00:38:21,520
+بايتكس فاستهما لسه الـ VCN-Vancomycin-Colistin
+
+522
+00:38:21,520 --> 00:38:26,980
+-Nystatin لنطلع وحفظينهم يعني ويمكن نميح على ال
+
+523
+00:38:26,980 --> 00:38:33,020
+chocolate agar أنا ذكرتلكوا خلال المحاضرة أنها
+
+524
+00:38:33,020 --> 00:38:40,640
+تتطلب الـ CO2 صح؟ و الـ CO2 بيكون فيه جار خاص
+
+525
+00:38:40,640 --> 00:38:45,300
+يسميها CO2 jar خلينا نربط معلومة بمعلومة و نروح
+
+526
+00:38:45,300 --> 00:38:49,060
+لمختبر كل الميكروبات
+
+527
+00:38:51,680 --> 00:38:54,840
+لأن في مشروطين كلمة مشروطين يعني بدها special
+
+528
+00:38:54,840 --> 00:38:57,680
+environments أو special conditions زي الـ Brucella
+
+529
+00:38:57,680 --> 00:39:01,740
+مثلا واحدة ست أسابيع بدها مش ممكن أزرعها مع
+
+530
+00:39:01,740 --> 00:39:04,740
+بكتيريا تانية هاتولي طبق ممكن يصمم ست أسابيع من
+
+531
+00:39:04,740 --> 00:39:07,400
+غير ما ينشف في الـ incubator ما فيش إمكانية
+
+532
+00:39:07,400 --> 00:39:10,960
+فـ Brucella بنزرعها بطريقة مختلفة تماما عن الـ other
+
+533
+00:39:10,960 --> 00:39:15,600
+samples كل بكتيريا الروتينية اللي بتحتاج CO2
+
+534
+00:39:15,600 --> 00:39:19,180
+بنزرعها على Chocolate agar حتى وإن كانت تنمو على برضه
+
+535
+00:39:19,180 --> 00:39:22,760
+agar ما هو اللي بتنمو على agar اللي هتنمو على مين؟
+
+536
+00:39:22,760 --> 00:39:25,940
+على الـ Chocolate agar فبنزرعها و بنميها على الـ
+
+537
+00:39:25,940 --> 00:39:28,560
+Chocolate agar و بنحطها في الـ CO2 إذا غالبا في
+
+538
+00:39:28,560 --> 00:39:32,440
+المعمل هتكون عندكوا طبق اسمه الـ Chocolate agar وين
+
+539
+00:39:32,440 --> 00:39:37,800
+بتتم زراعته أو وين بتتم تحضينه عفوا under CO2
+
+540
+00:39:37,800 --> 00:39:42,160
+conditions طبعا هنا فيه أنظمة CO2 incubation
+
+541
+00:39:44,710 --> 00:39:50,170
+هو مختبر الوايكل بتشوفه فيه نان انكبيتر فور بعض
+
+542
+00:39:50,170 --> 00:39:56,930
+التحتاني هذا الـ CO2 الكبير هذا الـ CO2 incubator هو
+
+543
+00:39:56,930 --> 00:40:01,150
+عبارة عن incubator عادي موصول باستوارة بتدخله CO2
+
+544
+00:40:01,150 --> 00:40:07,130
+بنسبة وفي دي water sensor إذا قلت النسبة بيشغل إذا 
+
+545
+00:40:07,130 --> 00:40:09,110
+زالت النسبة بيوقف وانتهى الموضوع
+
+546
+00:40:15,080 --> 00:40:18,060
+Antibiotic therapy أنا قلت لك البكتيريا يعني من
+
+547
+00:40:18,060 --> 00:40:22,180
+الأنواع اللي أصبحت مقاومة لمين؟ للبنسيلينات لذلك
+
+548
+00:40:22,180 --> 00:40:26,480
+بنلجأ إنه بنستخدم Antibiotic اسمه Ceftriaxone
+
+549
+00:40:26,480 --> 00:40:29,800
+و Ceftriaxone يتبع مجموعة اسمها Cephalosporin
+
+550
+00:40:29,800 --> 00:40:36,300
+و Cephalosporin هي عبارة عن drugs أو Antibiotics في
+
+551
+00:40:36,300 --> 00:40:39,120
+جزء منها مقاوم لـ Beta-lactamase إيش الـ Beta
+
+552
+00:40:39,120 --> 00:40:39,840
+-lactamase؟
+
+553
+00:40:42,170 --> 00:40:45,150
+اللي تنتجها البكتيريا عشان تكسر الـ beta-lactam
+
+554
+00:40:45,150 --> 00:40:48,170
+Antibiotic تدخل البنسلين في الـ beta-lactam
+
+555
+00:40:48,170 --> 00:40:53,210
+Antibiotic البنسلين و الـ Cephalosporins كيميائيا
+
+556
+00:40:53,210 --> 00:40:57,270
+بيحتووا على ما يسمى Beta-lactam ring Beta-lactam
+
+557
+00:40:57,270 --> 00:41:03,730
+ring نايت من أول بنسالين و Cephalosporins بيحتووا
+
+558
+00:41:03,730 --> 00:41:07,850
+على Beta-lactam ring كمكون أساسي وبتصنفهم على
+
+559
+00:41:07,850 --> 00:41:11,030
+إيه؟ Beta-lactam antibiotics Beta-lactam
+
+560
+00:41:11,030 --> 00:41:14,020
+antibiotics Chemically they are different هذا
+
+561
+00:41:14,020 --> 00:41:16,780
+Penicillins و هذا Cephalosporins حتى الـ
+
+562
+00:41:16,780 --> 00:41:20,680
+Cephalosporins في أصناف و أنواع كتيرة منها و كانت
+
+563
+00:41:20,680 --> 00:41:24,440
+الأولى بالنسبة لمين؟ البنسلين البكتيريا بتقاوم
+
+564
+00:41:24,440 --> 00:41:28,640
+Antibiotic ممكن تقاومه عن طريق إنتاج إنزيم يكسر
+
+565
+00:41:28,640 --> 00:41:33,160
+الـ Antibiotic فالـ Beta لك تميز لحظة بالجامعة
+
+566
+00:41:35,300 --> 00:41:39,300
+وهي مش مولودة هنا Beta-Lactamase لما بتتكلم عن
+
+567
+00:41:39,300 --> 00:41:41,980
+إنزيم إنزيم بتقول Beta-Lactamase يعني بقى بتحكي
+
+568
+00:41:41,980 --> 00:41:47,080
+إنه هي more than one Beta-lactam enzyme فهناك
+
+569
+00:41:47,080 --> 00:41:50,980
+Antibiotic بتتأثر بـ Beta-Lactamase و بتصير
+
+570
+00:41:50,980 --> 00:41:55,920
+ineffective فالبكتيريا بتصير الآن مقاومة عشان أقدر
+
+571
+00:41:55,920 --> 00:41:59,840
+أقتل البكتيريا هذه لازم أستخدم Cephalosporin أو
+
+572
+00:41:59,840 --> 00:42:03,540
+Penicillin مقاوم لـ Beta-Lactamase تبع البكتيريا
+
+573
+00:42:04,260 --> 00:42:06,660
+يبقى جيش اسمه الـ Beta-Lactamase resistant
+
+574
+00:42:06,660 --> 00:42:11,080
+Cephalosporin مقاوم لمين؟ للـ Beta-Lactamase اللي
+
+575
+00:42:11,080 --> 00:42:14,640
+هي تستخدمه لمقاومة اللي هو الـ Beta-lactam
+
+576
+00:42:14,640 --> 00:42:18,120
+Antibiotic مثل علي safe reaction قبل تكون الصورة
+
+577
+00:42:18,120 --> 00:42:24,600
+واضحة إنه هناك بعض الـ Antibiotic بدأت تتأثر سلبا
+
+578
+00:42:24,600 --> 00:42:27,920
+بإنزيم بتتكسر يعني بفعل إنزيم اسمه Beta
+
+579
+00:42:27,920 --> 00:42:30,890
+-Lactamase عشان أقدر أعالج أنا بكتيريا بيها لازم
+
+580
+00:42:30,890 --> 00:42:35,070
+يكون عندي مقاوم لهدا الـ Beta-lactam أو الـ Beta
+
+581
+00:42:35,070 --> 00:42:39,430
+-Lactamase الـ Resistance strains common they
+
+582
+00:42:39,430 --> 00:42:42,890
+produce Beta-Lactamase زي ما ذكرت Beta-Lactamase
+
+583
+00:42:42,890 --> 00:42:49,490
+map and destroy Penicillin الميكروب التاني في
+
+584
+00:42:49,490 --> 00:42:52,530
+السلسلة اللي هو Neisseria meningitidis أو Meningitis
+
+585
+00:42:52,530 --> 00:42:58,010
+أو اللي زي ما قلنا بندلع Mening و Coccus وشكل
+
+586
+00:42:58,010 --> 00:42:58,470
+كتير حلو
+
+587
+00:43:05,570 --> 00:43:11,490
+بالتسجيل هتشوفوها واضحة لأن بيصوروها من الجهاز
+
+588
+00:43:11,490 --> 00:43:16,170
+Neisseria meningitidis أو Meningitis Man is the only natural
+
+589
+00:43:16,170 --> 00:43:21,370
+known host برضه الإنسان هو الـ host الطبيعي المعروف
+
+590
+00:43:21,370 --> 00:43:24,710
+usually sporadic cases may cause epidemics
+
+591
+00:43:30,700 --> 00:43:37,700
+epidemics يعني بشكل وبائي يعني انتشار أكثر من
+
+592
+00:43:37,700 --> 00:43:43,880
+المعهود mostly young children هم الـ Host و الـ
+
+593
+00:43:43,880 --> 00:43:48,320
+population لكن الـ outbreaks ممكن تحدث لـ adults و
+
+594
+00:43:48,320 --> 00:43:52,140
+خاصة في الـ crowded conditions مثلا army barracks
+
+595
+00:43:52,140 --> 00:43:56,300
+barracks للجنود خاصة اللي بيجيبوهم جداد يسموهم
+
+596
+00:43:56,300 --> 00:44:01,150
+recruits المجندين الجدد المجندين الجدد بره في بعض
+
+597
+00:44:01,150 --> 00:44:04,270
+الجامعات أو هتفضل عربية في ما يسمى بسكن الطلبة،
+
+598
+00:44:04,270 --> 00:44:08,250
+سكن الطلبة، برضه الطلبة الجدد اللي بيجي بيسكنوا
+
+599
+00:44:08,250 --> 00:44:11,890
+في الجامعات و ممكن يسكنهم out breaks طيب خلينا
+
+600
+00:44:11,890 --> 00:44:16,150
+نستغل الفرصة و نتكلم عن Neisseria meningitidis من
+
+601
+00:44:16,150 --> 00:44:22,750
+خلال الـ army barracks و الـ college students أنتو
+
+602
+00:44:22,750 --> 00:44:27,450
+كلكوا سكان قطاع غزة، ماحدا جاي من برا؟ لما احنا
+
+603
+00:44:27,450 --> 00:44:32,510
+طلعنا برا ندرس طبعا، طلعنا كان فيه من الأردن و من
+
+604
+00:44:32,510 --> 00:44:35,950
+سوريا و من العراق، من دول مختلفة و جايين كلنا كلنا
+
+605
+00:44:35,950 --> 00:44:40,900
+على دول اسمها الفلبين، قعدنا ندرس فيها عارفين اللي
+
+606
+00:44:40,900 --> 00:44:44,280
+سافر أول اللي ... اللي ... اللي بسافر و هو صغير خاصة
+
+607
+00:44:44,280 --> 00:44:47,460
+في حالة يسموها homesickness homesickness يعني
+
+608
+00:44:47,460 --> 00:44:51,680
+بيصير حنين للوطن بعد ما كان بده يعني بأي وسيلة يطلع
+
+609
+00:44:51,680 --> 00:44:54,920
+بيصير حنين و ممكن يتكلم على هذا الأوضاع و يبقى بيبكي
+
+610
+00:44:54,920 --> 00:45:00,460
+يعني عشان هو غادر ال ... ال ... الأحد دولة فبيشعر
+
+611
+00:45:00,460 --> 00:45:03,740
+أول حاجة رقم واحد بيشعر إنه homesickness النفسية
+
+612
+00:45:03,740 --> 00:45:07,400
+بتبدء بتكون في depression لما يكون في depression
+
+613
+00:45:08,180 --> 00:45:11,360
+بيكون فيه low immunity خدوا بالكم low immunity هذا
+
+614
+00:45:11,360 --> 00:45:16,400
+واحد رقم اثنين جايين من مناطق مختلفة و سكننا مع
+
+615
+00:45:16,400 --> 00:45:20,780
+بعض سكن مع بعض مجموعة العادات الغذائية تبعتنا
+
+616
+00:45:20,780 --> 00:45:24,580
+مختلفة عن المكان و عن بعضنا البعض و الميكروبات
+
+617
+00:45:24,580 --> 00:45:29,300
+اللي في أنوفنا أو على جلدنا برضه مختلفة فغالبا في
+
+618
+00:45:29,300 --> 00:45:33,320
+التجمعات اللي من هذا النوع زي الجنود زي ما قلنا أو
+
+619
+00:45:33,320 --> 00:45:37,870
+الطلبة اللي ساكنين جداد في سكن جامعي بتوفر عندهم
+
+620
+00:45:37,870 --> 00:45:42,670
+ظروف من الـ crowdedness و الـ low immunity بحيث يسمح
+
+621
+00:45:42,670 --> 00:45:46,110
+لانتشار كثير من الأمراض، مش بس ... مش بس اللي هو الـ
+
+622
+00:45:46,110 --> 00:45:49,770
+... ال ... الـ cavalry unit أو الـ army barracks زمان كان
+
+623
+00:45:49,770 --> 00:45:53,830
+الحجاج أكثر ناس إصابة بمين؟ بال ... بالـ microbial،
+
+624
+00:45:53,830 --> 00:45:56,970
+لذلك أي واحد، لأن من فلسطين أو من باكرا، حاجة
+
+625
+00:45:56,970 --> 00:45:59,670
+عالية، ما بيقدرش بياخد الـ vaccine تبع مين؟ تبع أن
+
+626
+00:45:59,670 --> 00:46:04,450
+سوريا من ال device، تمام؟ يبقى لما أنا قلت في أول
+
+627
+00:46:04,450 --> 00:46:08,410
+محاضرة susceptible population army recruits
+
+628
+00:46:08,410 --> 00:46:13,370
+college students are susceptible groups طبعا
+
+629
+00:46:13,370 --> 00:46:20,570
+susceptible groups طيب غالبا كيف جثة حياة
+
+630
+00:46:20,570 --> 00:46:24,590
+البكتيريا دي؟ هي موجودة للأسف كمان هنا فاكرين
+
+631
+00:46:24,590 --> 00:46:29,680
+نظرية روبرت كوخ فرضية روبرت كوخ لما كنا يجب أن يكون
+
+632
+00:46:29,680 --> 00:46:35,220
+ال microbe في الحيوان المصاب و ليس السليم كنا هاد
+
+633
+00:46:35,220 --> 00:46:39,580
+المبادئ للأسف X لأن هي مثلا نيسيريا مينينجايتديس
+
+634
+00:46:39,580 --> 00:46:44,100
+can be present in up to 15% of the healthy
+
+635
+00:46:44,100 --> 00:46:47,780
+population يعني لو ميا احنا هنا أخدنا من أنوفهم
+
+636
+00:46:47,780 --> 00:46:53,260
+مسحات هنجي على الأقل في 15% منهم نيسيريا مينينجايتديس 
+
+637
+00:46:53,260 --> 00:46:55,600
+داينس normal microbiome
+
+638
+00:46:58,170 --> 00:47:00,910
+طب what .. what .. what goes wrong؟ قلنا ال immune
+
+639
+00:47:00,910 --> 00:47:06,550
+system بيكون انخفض، بيكون انخفض فبتدى، أول حاجة
+
+640
+00:47:06,550 --> 00:47:09,310
+بتعملها بتعمل colonization في ال nasopharynx،
+
+641
+00:47:09,310 --> 00:47:14,790
+attachment and colonization، بعدين can reach the
+
+642
+00:47:14,790 --> 00:47:19,050
+bloodstream إذا وصلنا للدم في condition اسمه
+
+643
+00:47:19,050 --> 00:47:21,930
+septicemia بتبقى نقوله بس عشان نميز ال septicemia
+
+644
+00:47:21,930 --> 00:47:24,570
+تبعتها عن أي نوع من ال septicemia بسموها
+
+645
+00:47:24,570 --> 00:47:32,230
+meningococcemia meningococcemia وهي من أخطر أنواع
+
+646
+00:47:32,230 --> 00:47:37,270
+ال septicemia و كثير من المرضى بيموتوا عند البرحلة
+
+647
+00:47:37,270 --> 00:47:43,290
+هذه meningococcemia بصير عنده بُقع دموية بسموها
+
+648
+00:47:43,290 --> 00:47:44,370
+petechial rashes
+
+649
+00:47:47,040 --> 00:47:50,600
+يعني بيصير فيه disseminated intravascular .. مر
+
+650
+00:47:50,600 --> 00:47:53,340
+معاك بالمصطلح disseminated intravascular
+
+651
+00:47:53,340 --> 00:47:59,000
+coagulation تخطرات منتشرة في كل الجسم تمام؟ بتسكر
+
+652
+00:47:59,000 --> 00:48:02,440
+الكابليريز أو بتنفجر الكابليريز تمام؟ في عندنا
+
+653
+00:48:02,440 --> 00:48:06,720
+مجموعة من الأعراض اللي بتنتجها هذه البكتيريا ما بتش
+
+654
+00:48:06,720 --> 00:48:11,160
+المريض من .. من الجوكسيميا تتطور و تروح على ال
+
+655
+00:48:11,160 --> 00:48:15,520
+brain و بتعملنا ال meningitis تمام؟ و تذكره دائما
+
+656
+00:48:16,190 --> 00:48:21,910
+إذا الـ meningitis أي خلية ماتت لن تعوض، ما عندناش
+
+657
+00:48:21,910 --> 00:48:24,770
+regeneration اللي خلالها ال nerve cell صح ولا لأ؟
+
+658
+00:48:24,770 --> 00:48:29,050
+ما عندناش regeneration، هو بالتالي ال damage بيكون
+
+659
+00:48:29,050 --> 00:48:32,490
+يعني كبير لما المرض بيكون ميت، عشان هيك اللي بنقول
+
+660
+00:48:32,490 --> 00:48:35,110
+دايما عينة ال CSF يجب أن تعمل في المختبر ك
+
+661
+00:48:35,110 --> 00:48:39,570
+emergency، يترك العينة الأخرى، يترك أخرى، أقول لكم
+
+662
+00:48:40,350 --> 00:48:43,730
+في الأماكن اللي بتحترم نفسها قبل ما يسحبوا العينة
+
+663
+00:48:43,730 --> 00:48:46,410
+بتتصلوا في المختبر تقولوا لهم جاهزوا حالكم فيه أن
+
+664
+00:48:46,410 --> 00:48:51,990
+عينات CSF حتى يعني إذا كان من الجامعة على وصولها
+
+665
+00:48:51,990 --> 00:48:56,710
+كان في ده ناشي نخلصه و نتفرج انتظارا ل .. لذلك في
+
+666
+00:48:56,710 --> 00:49:02,430
+ال CSF بدنا نعرف كمان إنه كل ما قصرنا فترة التشخيص
+
+667
+00:49:02,430 --> 00:49:08,660
+كل ما كان عندنا ال .. ال damage أقل لأنه تخيلوا أن
+
+668
+00:49:08,660 --> 00:49:13,420
+أنا أعمل مزرعة بتنتظر 72 ساعة عشان أعطي مريض نتائج
+
+669
+00:49:13,420 --> 00:49:17,760
+و sensitivity لأ هم بياخدوا العينة و بيبدأوا علاج
+
+670
+00:49:17,760 --> 00:49:23,920
+based on best guess أفضل تخمين .. أفضل تخمين ..
+
+671
+00:49:23,920 --> 00:49:26,900
+تخمين الطبيب غير تخمين الراجل من الشارع التخمين
+
+672
+00:49:26,900 --> 00:49:30,900
+بيكون عادي عن مين؟ عن .. عن clinical picture أنه
+
+673
+00:49:30,900 --> 00:49:33,120
+.. و غير ما بيعطوا broad spectrum في هذه الحياة
+
+674
+00:49:33,120 --> 00:49:39,730
+لحين ظهور من نتائج المزرعة في عندنا دخول عبر ال
+
+675
+00:49:39,730 --> 00:49:41,770
+respiratory tract و بعدين على ال blood stream و
+
+676
+00:49:41,770 --> 00:49:44,670
+بعدين على ال central nervous system في أنا حاضر في
+
+677
+00:49:44,670 --> 00:49:49,530
+يوتيوب اثنين يوتيوب ياريت تتفرجوا عليهم في المنزل من
+
+678
+00:49:49,530 --> 00:49:52,610
+المحاضرة المصورة أو من ال presentation موجودة عندك
+
+679
+00:49:52,610 --> 00:49:54,130
+و ده موجودة عندك تطلع عليه
+
+680
+00:49:57,420 --> 00:50:00,420
+ال short period تبعتها ال incubation من واحد لأربعة
+
+681
+00:50:00,420 --> 00:50:03,360
+أيام second most common meningitis طبعا ال
+
+682
+00:50:03,360 --> 00:50:06,240
+pneumococcus most common بس خدوا بالكم كلمة most
+
+683
+00:50:06,240 --> 00:50:10,360
+common و second most common هذه متغيرة و country
+
+684
+00:50:10,360 --> 00:50:13,900
+specific يعني بمعنى آخر ممكن تكون number one في
+
+685
+00:50:13,900 --> 00:50:18,520
+بلد number two في بلد ثانية ليش أنا أقول لكم الكلام
+
+686
+00:50:18,520 --> 00:50:22,930
+هذا لأن مثلا في الـ 2011 دخلنا ال .. ال .. ال .. ال
+
+687
+00:50:22,930 --> 00:50:25,310
+.. ال vaccine تبع الـ streptococcus pneumoniae أكيد
+
+688
+00:50:25,310 --> 00:50:27,570
+ال Epidemiology تبع ال streptococcus pneumoniae
+
+689
+00:50:27,570 --> 00:50:31,770
+هيختلف، حد بطل ال number one، إذا بدنا نعرف إن هذا
+
+690
+00:50:31,770 --> 00:50:36,270
+مش قوانين مائة بالمائة فاتل
+
+691
+00:50:36,270 --> 00:50:38,870
+if untreated، فاتل إن القاتل لا يقدر أن يتعالج،
+
+692
+00:50:38,870 --> 00:50:41,910
+respond well to antibiotic therapy زي اللي هو ال
+
+693
+00:50:41,910 --> 00:50:47,450
+penicillin طبعا معلومة و نختم فيها ال penicillin
+
+694
+00:50:47,450 --> 00:50:54,790
+لا يستطيع اختراق الـ blood brain barrier لكن لما
+
+695
+00:50:54,790 --> 00:50:59,110
+يكون الـ meninges inflamed بيهارتها بأساسها
+
+696
+00:50:59,110 --> 00:51:04,890
+فالبنسيلين مجرد يخترق لذلك بنعالج بالبنسيلين و
+
+697
+00:51:04,890 --> 00:51:07,750
+بنمنع الكاريش أشعر فيه الكاريش اللي هو ال carrier
+
+698
+00:51:07,750 --> 00:51:11,830
+state بانتبايوتك آخر بانتبايوتك آخر فبنعالج
+
+699
+00:51:11,830 --> 00:51:15,270
+بالبنسيلين و بنمنع يعني بنضمن إن المرض هذا ما يعديش
+
+700
+00:51:15,270 --> 00:51:18,010
+للاخرين أو ما ينجلش إنه يسيريا من الديسن
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/EVwb_DZpLUo.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/EVwb_DZpLUo.srt
new file mode 100644
index 0000000000000000000000000000000000000000..e2efb373114e9632a470c4ae076722209d0ce83a
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/EVwb_DZpLUo.srt
@@ -0,0 +1,2409 @@
+1
+00:00:20,640 --> 00:00:24,040
+بسم الله الرحمن الرحيم استكمال اللي مبلقناها من
+
+2
+00:00:24,040 --> 00:00:28,840
+الـ Gram-Negative Bacilli  و أذكركم بالتفصيلات اللي
+
+3
+00:00:28,840 --> 00:00:31,760
+في التصنيفات اللي عملناها في الـ .. الـ .. الـ Gram 
+
+4
+00:00:31,760 --> 00:00:38,150
+Negative Bacilli بناء على فحصين الـ Oxidase قسمنا
+
+5
+00:00:38,150 --> 00:00:41,810
+البكتيريا إلى أربع أصناف، الصنف في الأول كان 
+
+6
+00:00:41,810 --> 00:00:45,230
+Oxidase positive، glucose positive، fermenter
+
+7
+00:00:45,230 --> 00:00:48,430
+يعني، وكان هو الـ Vibrio وعلى النقيد من الجهة
+
+8
+00:00:48,430 --> 00:00:51,210
+التانية Oxidase negative، glucose negative،
+
+9
+00:00:51,210 --> 00:00:55,530
+Acinetobacter وفي النص Oxidase negative، glucose
+
+10
+00:00:55,530 --> 00:00:59,750
+positive، Enterobacteriosis Oxidase positive،
+
+11
+00:00:59,750 --> 00:01:05,050
+glucose negative، Pseudomonas الجزء الأكبر من
+
+12
+00:01:05,050 --> 00:01:08,130
+مفاهيمنا السابق كانت مركزة كلها تقريبا على الـ
+
+13
+00:01:08,130 --> 00:01:12,570
+Enterobacteriaceae  خدنا منها أذكركم أخدنا
+
+14
+00:01:12,570 --> 00:01:15,890
+الإشريشيا، السالمونيلا، الشيجيلا، شوية عن
+
+15
+00:01:15,890 --> 00:01:21,030
+الكليبسيلة، و شوية عن البروتوس أمام؟ اليوم هنتكلم
+
+16
+00:01:21,030 --> 00:01:24,930
+عن المجموعات الأخرى اللي هم الـ Vibrio و الـ
+
+17
+00:01:24,930 --> 00:01:29,230
+Pseudomonas و يمكن نمر على الـ Acetobacter الصورة
+
+18
+00:01:29,230 --> 00:01:36,710
+اللي قدامكم تبين بكتيريا منحنية، و في إلها Flagella
+
+19
+00:01:36,710 --> 00:01:42,330
+و هذه الصورة غالبا نعطيها الـ Vibrio اللي معروف
+
+20
+00:01:42,330 --> 00:01:47,830
+عنها Coma shape و Motile Bipolar Flagella الـ
+
+21
+00:01:47,830 --> 00:01:52,310
+Vibrio cholera الرمز تبعها حرف C اللي هو بالفعل
+
+22
+00:01:52,310 --> 00:01:57,390
+Curve بس لاي Curve بس لاي لو طلع الصورة قدامنا
+
+23
+00:01:57,390 --> 00:02:03,460
+بتوضحلنا المعنى الحقيقي لكلمة Cholera Cholera هو
+
+24
+00:02:03,460 --> 00:02:08,300
+مرض Gastrointestinal Tract Infection characterized
+
+25
+00:02:08,300 --> 00:02:12,760
+by severe diarrhea and severe dehydration و الـ
+
+26
+00:02:12,760 --> 00:02:16,240
+Dehydration هو اللي بيؤدي بنا إلى الوفيات في
+
+27
+00:02:16,240 --> 00:02:20,380
+الأطفال، هنشوف كمان شوية وين منتشر الـ Cholera و لحد
+
+28
+00:02:20,380 --> 00:02:25,600
+الآن في عالمنا الحالي وين الـ Cholera موجودة، لكن
+
+29
+00:02:25,600 --> 00:02:26,460
+تذكروا دائما 
+
+30
+00:02:32,210 --> 00:02:35,650
+و الجملة الذهبية اللي قلنا عنها اللي هو الـ Safe أو
+
+31
+00:02:35,650 --> 00:02:40,850
+الـ Unsafe Drinking Water يعني مياه شرب غير آمنة
+
+32
+00:02:40,850 --> 00:02:45,930
+غير نظيفة توقعوا أن يكون فيه انتشار لمرض الكوليرا هل
+
+33
+00:02:45,930 --> 00:02:52,630
+في فلسطين احنا محميين من مرض الكوليرا قبل يعني حوالي
+
+34
+00:02:52,630 --> 00:02:58,790
+ثلاثة عشر عام أو أربعة عشر عام صار هنا وباء في قطاع غزة
+
+35
+00:02:59,780 --> 00:03:04,160
+هذا الوباء يعني لم يطل بالزمان وحوصر الحمد لله رب
+
+36
+00:03:04,160 --> 00:03:07,760
+العالمين وربنا نجانا منه يعني نسبة الوفيات كانت في
+
+37
+00:03:07,760 --> 00:03:11,980
+أظن حالة أو حالتين فقط توفوا وباقي الحالات شفيت
+
+38
+00:03:11,980 --> 00:03:15,940
+بأمر الله سبحانه وتعالى ومن ثم بجهود المخلصين من
+
+39
+00:03:15,940 --> 00:03:19,000
+وزارة الصحة ووكالة الغوث وحتى المؤسسات التعليمية
+
+40
+00:03:19,000 --> 00:03:23,880
+أيامها ساهمت في الحد من انتشار هذا المرض
+
+41
+00:03:27,460 --> 00:03:32,420
+يعني لو توفرت الظروف مرة أخرى قد يحدث مرة أخرى، و
+
+42
+00:03:32,420 --> 00:03:35,480
+تتذكر محاضرة السابق لما تكلمنا عن الحروب وما هي
+
+43
+00:03:35,480 --> 00:03:41,520
+أسبابها من اختلاف في مصادر المياه ونوعية هذه
+
+44
+00:03:41,520 --> 00:03:48,140
+المياه، القصة تبدأ دائما وأبدا بـ Drinking of Dirty
+
+45
+00:03:48,140 --> 00:03:53,880
+أو Contaminated Water واحنا طبعا الماء اللي بيصلنا
+
+46
+00:03:53,880 --> 00:03:57,040
+إلى المنزل اللي بيصلنا ما بنعرفش من مين مصدره،
+
+47
+00:03:57,040 --> 00:04:00,100
+عارفين من أين جاية؟ من كوكب المريخ ولا من .. مش
+
+48
+00:04:00,100 --> 00:04:02,940
+عارفين أنتو من أين جاية، لكن في النهاية في جهة
+
+49
+00:04:02,940 --> 00:04:07,490
+مسئولة عن إيصاله، وهذا الجهة Is Responsible .. Is
+
+50
+00:04:07,490 --> 00:04:10,570
+Responsible، و أنا حكيتلكوا المرة الماضية أنه كل
+
+51
+00:04:10,570 --> 00:04:16,310
+البلديات تخضع لعملية فحص دورية في مختبر الصحة
+
+52
+00:04:16,310 --> 00:04:19,470
+العامة، بمعنى أن الماء اللي بيصنع غالبا بيكون
+
+53
+00:04:19,470 --> 00:04:24,370
+Politically Checked لكن في مناطق زي الصورة اللي
+
+54
+00:04:24,370 --> 00:04:28,910
+بتاعة اللي مصدر مياه الشرب تبعها ممكن يكون River
+
+55
+00:04:28,910 --> 00:04:36,160
+يعني مصدر عام، وأنا بقول دائما أن الإنسان على مدار
+
+56
+00:04:36,160 --> 00:04:40,380
+التاريخ أينما ورد الماء ورد الإنسان وورد الحضارة، و
+
+57
+00:04:40,380 --> 00:04:43,820
+أينما ورد الإنسان وردت الحضارة، تلوث الماء معروفة
+
+58
+00:04:43,820 --> 00:04:47,460
+القصة لأنه بيلجأ الماء الجاري، فبيصير يتخلص من
+
+59
+00:04:47,460 --> 00:04:51,040
+النفايات تبعته في المياه الجارية، صحيح المياه
+
+60
+00:04:51,040 --> 00:04:56,640
+الجارية إلها قدرة تطهير ذاتي زي البحر مثلا، لكن
+
+61
+00:04:56,640 --> 00:05:02,770
+كمان مرة قدرة التطهير محدودة، بمعنى أنه لا تستطيع ..
+
+62
+00:05:02,770 --> 00:05:06,910
+إذا تجاوزنا هذه القدرة، فيصبح هناك أثر اسمه
+
+63
+00:05:06,910 --> 00:05:10,950
+Contamination و Pollution وهيحيطش عنه أمراض تنتقل
+
+64
+00:05:10,950 --> 00:05:18,790
+إلى الإنسان، صورة أخرى تعبر، هذه الصورة اللي في
+
+65
+00:05:18,790 --> 00:05:23,140
+الأعلى واضح أن فيه مياه Sewage وفي نفس اللي وجدت
+
+66
+00:05:23,140 --> 00:05:26,960
+فيه مواسير أو اللي هي القنابيب اللي فيها مياه
+
+67
+00:05:26,960 --> 00:05:29,840
+الشرب، و الأطفال بيلهوا في هذه المنطقة فبيختلف أكيد
+
+68
+00:05:29,840 --> 00:05:33,140
+مياه الشرب مع مياه اللي هو الـ Sewage الصورة اللي
+
+69
+00:05:33,140 --> 00:05:38,400
+أسفل بتوضح أن لما يكون الاستخدام متعدد لنفس
+
+70
+00:05:38,400 --> 00:05:42,220
+المصدر تبع المياه في منطقة، وهو المنطقة اللي قدامي
+
+71
+00:05:42,220 --> 00:05:44,900
+شوية بيكون بيستخدموا مياه الشرب وبالتالي الـ
+
+72
+00:05:44,900 --> 00:05:49,960
+Pollution بينتقل من المنطقة إلى منطقة أخرى، طيب،
+
+73
+00:05:49,960 --> 00:05:52,820
+رأينا ما يكفي من الصور، نبدأ الآن نتكلم عن
+
+74
+00:05:52,820 --> 00:05:56,840
+المعلومات الـ Vibrio في شكل عام، مش الكوليرا، هي
+
+75
+00:05:56,840 --> 00:06:00,920
+Gram Negative بس لاي، هنوانها Curved or Comma
+
+76
+00:06:00,920 --> 00:06:03,640
+Shaped Nonsense Performing، كل المعلومات لحد الآن
+
+77
+00:06:03,640 --> 00:06:07,500
+بسيطة ومعروفة، Highly Motile، لكن الموتوري تبعتها
+
+78
+00:06:07,500 --> 00:06:11,400
+الآن بدأت أن تختلف، كنا بالأول نتكلم عن Peritrichous
+
+79
+00:06:11,400 --> 00:06:13,540
+Flagella في الـ Vibrio Cholera، الآن نتكلم عن
+
+80
+00:06:13,540 --> 00:06:19,090
+Single Polar Flagella It is associated with salt
+
+81
+00:06:19,090 --> 00:06:21,870
+water أول بكتيريا من دروسنا اللي لها علاقة بالمياه
+
+82
+00:06:21,870 --> 00:06:26,530
+المالحة، يعني البحر، ممكن ينجل من هنا، يعني مش هتموت
+
+83
+00:06:26,530 --> 00:06:29,230
+في البحر، مش هتموت في البحر اللي أنا قاعد بقولكوا
+
+84
+00:06:29,230 --> 00:06:33,470
+أنه عنده قدرة تطهير لأنه عندها Tolerance كويس
+
+85
+00:06:33,470 --> 00:06:38,410
+للملح، عندها Tolerance يعني تحمل للملوحة It is
+
+86
+00:06:38,410 --> 00:06:44,370
+Oxidized Positive يعني برضه كمان اختلاف عن الـ Entero
+
+87
+00:06:44,370 --> 00:06:45,810
+بكتيريا اللي كنا ندرسها قبل قليل
+
+88
+00:06:50,030 --> 00:06:55,010
+Tolerate Alkaline هذه أول صفة أو أهم صفة في الـ
+
+89
+00:06:55,010 --> 00:06:58,550
+Vibrio على الإطلاق أن It can tolerate alkaline
+
+90
+00:06:58,550 --> 00:07:02,850
+Conditions وبالتالي وبالتالي بدنا نستغل هذه الصفة
+
+91
+00:07:02,850 --> 00:07:06,210
+To selectively isolate this organism أنتو عارفين
+
+92
+00:07:06,210 --> 00:07:10,950
+بتتكلم عن Sewage أو Stool أو Water وكل المصادر اللي
+
+93
+00:07:10,950 --> 00:07:13,630
+ذكرتهم أنا بيحتووا على مش Single Microbe بيحتووا
+
+94
+00:07:13,630 --> 00:07:17,240
+على Multiple Microbes To selectively isolate Vibrio
+
+95
+00:07:17,240 --> 00:07:21,120
+You should provide alkaline conditions لأن هي
+
+96
+00:07:21,120 --> 00:07:25,240
+الوحيدة اللي Could Thrive أو Survive والباقية
+
+97
+00:07:25,240 --> 00:07:28,380
+هيموتوا فبالتالي بنعتبرها Enrichment أو Selective
+
+98
+00:07:28,380 --> 00:07:34,540
+Media للـ Vibrio كمان هاد المثال طبعا في تفسير كمان
+
+99
+00:07:34,540 --> 00:07:40,160
+شوية هيمروا معانا معلومة بتقول أن الجرعة المؤدية
+
+100
+00:07:40,160 --> 00:07:45,830
+لإحداث المرض من عشرين إلى مائة، هذه بدها من مائة مليون
+
+101
+00:07:45,830 --> 00:07:51,910
+إلى ألف مليون، مائة مليون خلية عشان نفسر عنها كلها،
+
+102
+00:07:51,910 --> 00:07:58,850
+طبعا؟ معناه الحكي .. بنفسر هذا الكلام لأن الـ
+
+103
+00:07:58,850 --> 00:08:02,630
+Bacteria Very Sensitive to the Stomach Acid فمعظم
+
+104
+00:08:02,630 --> 00:08:07,270
+الجرع اللي بناخدها بتموت عشان تعطي فرصة للـ Survival
+
+105
+00:08:07,270 --> 00:08:11,530
+كل مزارعة البكتيريا اللي داخل كل مزارعة فرصتها أن
+
+106
+00:08:11,530 --> 00:08:14,010
+Two or Three Organisms to Survive to the
+
+107
+00:08:14,010 --> 00:08:17,550
+Intestinal Tract فمعظم الـ Vibrio بتموت وين؟ في الـ
+
+108
+00:08:17,550 --> 00:08:21,470
+Stomach من اللي هو الـ Acid لأنها بتفضل الظروف من
+
+109
+00:08:21,470 --> 00:08:25,110
+كم مرة إلى الكوليرا، هذا واحد، رقم اثنين ممكن نستفيد من
+
+110
+00:08:25,110 --> 00:08:30,850
+المعلومة بأن لو في زمن الكوليرا في زمن الكوليرا أو
+
+111
+00:08:30,850 --> 00:08:33,890
+الوباء تبع الكوليرا إن لو بدنا نوفر مياه نظيفة لو
+
+112
+00:08:33,890 --> 00:08:36,730
+ما عندناش إمكانية لقتلها بالكلور، ممكن نقتلها بالـ
+
+113
+00:08:36,730 --> 00:08:43,410
+Acid ضفنا خل على الماء أو ضفنا ليمون، قطرات من
+
+114
+00:08:43,410 --> 00:08:46,110
+الليمون بيخفضوا البيئة وبالتالي بنقتل اللي هو الـ
+
+115
+00:08:46,110 --> 00:08:51,470
+Vibrio كلها، البكتيريا ده مش صعب أنه نميها يعني لما
+
+116
+00:08:51,470 --> 00:08:55,110
+احنا نازلينها على الـ Selective Media بناخدها و
+
+117
+00:08:55,110 --> 00:08:57,550
+بنزلها على بيئة أجار عادي، على بيئة أجار عادي،
+
+118
+00:08:57,550 --> 00:08:59,830
+على مقومكي عادي، لكن المشكلة وين أن؟ To
+
+119
+00:08:59,830 --> 00:09:04,290
+Selectively Isolate the Organism، أنه نازلينها من ..
+
+120
+00:09:04,290 --> 00:09:08,450
+من خلطة، وكنا على هذا الغلط، بدنا نستخدم اللي هو الـ
+
+121
+00:09:08,450 --> 00:09:15,450
+Alkaline pH Three Species من الـ Vibrio اللي بدنا
+
+122
+00:09:15,450 --> 00:09:20,490
+نتطلع عليهم طبعا احنا هنتطرق للثانية والثالثة
+
+123
+00:09:20,490 --> 00:09:24,790
+هي أقصى حد من المعلومات اللي هنعطيها للفصل هذا الـ
+
+124
+00:09:24,790 --> 00:09:28,030
+Vibrio Parahaemolyticus Which Causes Gastroenteritis اللي بسميه
+
+125
+00:09:28,030 --> 00:09:32,410
+احنا كلنا يعني من خطب مميزة عن بقية الـ 
+
+126
+00:09:32,410 --> 00:09:34,610
+gastroenteritis لأنها بالفعل مميزة
+
+127
+00:09:38,160 --> 00:09:45,080
+vibrio parahaemolyticus  و هذه بتنتقل mainly من تناول
+
+128
+00:09:45,080 --> 00:09:50,480
+الأسماك الغير مطهية أو غير المطبوخة، قاعدة بيأكل
+
+129
+00:09:50,480 --> 00:09:55,420
+سمك نهي؟ sushi، ففي ناس بيقولوا sushi قاعدين،
+
+130
+00:09:55,420 --> 00:10:00,620
+ثقافتهم اليابانيةاليابانيين وثقافتهم بالسوشي انتقلت
+
+131
+00:10:00,620 --> 00:10:03,980
+إلى كل العالم وصبحت عدوى موجودة في كل الدنيا
+
+132
+00:10:03,980 --> 00:10:06,880
+المطاعم اليابانية اللي بتقدم اللي هو السوشي أو
+
+133
+00:10:06,880 --> 00:10:11,580
+الطعام أو الأسماك اللي غير مطهية طبعا تتذكروا أن
+
+134
+00:10:11,580 --> 00:10:16,480
+أنا تكلمت أن ال Vibrio is associated with marine
+
+135
+00:10:16,480 --> 00:10:20,380
+أو salt water يعني بحرية وبالتالي ممكن تنتقل عن
+
+136
+00:10:20,380 --> 00:10:23,940
+طريق اللي هو الأسماك وبتعمل gastroenteritis, wound
+
+137
+00:10:23,940 --> 00:10:29,550
+infection and the last bacteria Vibrio vulnificus
+
+138
+00:10:29,550 --> 00:10:32,050
+بصحيح أنه هذي من ال .. يعني من الميكروبات اللي
+
+139
+00:10:32,050 --> 00:10:35,690
+بتعتبر يعني إيه جديد عرفنا أنها بتعمل infection،
+
+140
+00:10:35,690 --> 00:10:37,730
+كلمة جديدة يعني عشرين سنة، خمس وعشرين سنة يعني،
+
+141
+00:10:37,730 --> 00:10:39,750
+تمام؟ هي جديد معناها، لأن معظم ال micro ..
+
+142
+00:10:39,750 --> 00:10:44,170
+البكتيريا عرفناها في خمسينيات وقبل خمسينيات القرن
+
+143
+00:10:44,170 --> 00:10:48,690
+الماضي، تمام؟ بعد ذلك بتعتبروا أنها جديد Vibrio
+
+144
+00:10:48,690 --> 00:10:52,030
+vulnificus بتامل severe wound infection وبكتيريميا
+
+145
+00:10:52,030 --> 00:10:55,130
+خاصة عند الناس اللي بيشتغلوا في الأسماك، إيش اللي
+
+146
+00:10:55,130 --> 00:10:58,350
+بيشتغلوا في الأسماك؟ إما بيصيدوا أسماك أو بيرغفوا
+
+147
+00:10:58,350 --> 00:11:02,070
+الأسماك، في طبعا في المطابخ، في المطاعم، في
+
+148
+00:11:02,070 --> 00:11:04,810
+الأماكن الأسواق، من ببيع السمك، بينظفوا السمك،
+
+149
+00:11:04,810 --> 00:11:08,430
+فهدول الناس عرضة إنهم يصيبوا ال Vibrio vulnificus،
+
+150
+00:11:08,430 --> 00:11:11,530
+الأسماء مرة أخرى Vibrio cholera Vibrio
+
+151
+00:11:11,530 --> 00:11:16,650
+parahaemolyticus و Vibrio vulnificus الأولى أساسا ال
+
+152
+00:11:16,650 --> 00:11:19,650
+.. أساسا ال .. ال .. ال .. ال Vibrio وهي الأشهر
+
+153
+00:11:19,650 --> 00:11:23,490
+على الإطلاق وهي الأخطر على الإطلاق وتأثيرها الصحي
+
+154
+00:11:23,490 --> 00:11:28,710
+على المجتمعات والأخطر على مدار التاريخ في عندنا
+
+155
+00:11:28,710 --> 00:11:31,190
+مرحلة اسمها طاعون، كلكم بتعرفوا عنه، الدراسة طاعون
+
+156
+00:11:31,190 --> 00:11:36,250
+في التاريخ لما فشل حملة نابليون وفي طاعون مشهور
+
+157
+00:11:36,250 --> 00:11:41,350
+عندنا في فلسطين طاعون عمواس اللي استجت فيه أبو عبادة
+
+158
+00:11:41,350 --> 00:11:45,990
+عمر بن الجراح طاعون أو مؤسس شهير إن الطاعون مرض خطير
+
+159
+00:11:45,990 --> 00:11:50,790
+ومعروف وكانوا في .. في مناطق الزمنية اللي فيها جهة
+
+160
+00:11:50,790 --> 00:11:55,530
+الكبير لما يكون مرض قاتل يسموه طاعون حتى لو كانش
+
+161
+00:11:55,530 --> 00:11:59,170
+طاعون وفي أزمية من الأزمات كمان كانوا يسموا
+
+162
+00:11:59,170 --> 00:12:03,670
+الأمراض القاتلة يسموها cholera لأن ال cholera كانت
+
+163
+00:12:03,670 --> 00:12:07,400
+تقتل أيضا نسبة كبيرة من الناسفكانوا يقولوا طاعون أو
+
+164
+00:12:07,400 --> 00:12:10,740
+يقولوا كوليرا للدلالة على إن المرض يقتل أو نسبة
+
+165
+00:12:10,740 --> 00:12:13,920
+القتل فيه عالية جدا إذا مش المقصود في كل كوليرا
+
+166
+00:12:13,920 --> 00:12:17,640
+كانت تاريخيا إنها كوليرا وإنما ربما تكون مرض آخر
+
+167
+00:12:17,640 --> 00:12:21,740
+لكن نسبة القتل فيه عالية فأطلق عليه لقب كوليرا هذا
+
+168
+00:12:21,740 --> 00:12:24,900
+كلام قبل ما يتسجل العلم بال micro أو علم ال micro
+
+169
+00:12:24,900 --> 00:12:31,740
+virus طيب ال antigenic structure لل Vibrio cholera
+
+170
+00:12:32,790 --> 00:12:38,070
+تحتوي على two antigens مهمين جدا اللي هم ال O لأن
+
+171
+00:12:38,070 --> 00:12:41,790
+هي gram negative بس لله، فيها ليبوكوليسكارات وفيها
+
+172
+00:12:41,790 --> 00:12:46,830
+H لأن فيها polar flagella، تمام؟ وأن فيها حوالي 150
+
+173
+00:12:46,830 --> 00:12:53,140
+serotype، بناء على مين؟ بناء على ال O-antigen لكن
+
+174
+00:12:53,140 --> 00:12:58,180
+من هذه ال 150 فقط two serotypes اللي هم ال O1 وال
+
+175
+00:12:58,180 --> 00:13:04,700
+O139 هم اللي بيعملوا infection بالكوليرا للإنسان و
+
+176
+00:13:04,700 --> 00:13:08,040
+للأسف .. للأسف إحنا ظالمين الفيبروكوليرا طلعنا
+
+177
+00:13:08,040 --> 00:13:13,000
+فكرنا الفيبروكوليرا هي من رأسها بتسبب الكوليرا يعني
+
+178
+00:13:13,000 --> 00:13:16,920
+هي حابة تعمل معاها الكوليرا لكن واقع الأمر لجينا إنه
+
+179
+00:13:16,920 --> 00:13:22,280
+ال toxin اللي بيأهلها تعمل الكوليرا هو phage encoded
+
+180
+00:13:22,910 --> 00:13:28,690
+إيش يعني؟ يعني هي lysogenized lysogenized cells
+
+181
+00:13:28,690 --> 00:13:34,310
+يبقى عشانك تفسر أن لماذا ال 150 serotypes كلهم مش
+
+182
+00:13:34,310 --> 00:13:38,250
+مهمين، مين المهم فقط منهم اللي هو اللي فيها ال pro
+
+183
+00:13:38,250 --> 00:13:43,150
+-phage ال O1 وال O139 هم دول التانية اللي موجودة
+
+184
+00:13:43,150 --> 00:13:46,150
+فيهم ال bacteriophage طبعا هنمرّ على كده شوية في
+
+185
+00:13:46,150 --> 00:13:51,050
+أسماء حلوة هنسمعها الآن Ogawa
+
+186
+00:13:51,050 --> 00:13:57,330
+Inaba هيكوجيما إنابا وأجاوة وهيكوجيما three
+
+187
+00:13:57,330 --> 00:14:05,250
+serotypes لمن؟ لل Vibrio cholera لل O1 كلمة
+
+188
+00:14:05,250 --> 00:14:10,930
+serotype بقى فهمها إيش معناها إنه سلالة تختلف عن
+
+189
+00:14:10,930 --> 00:14:15,310
+سلالة أخرى عن طريق الأنتيجين تبعتها صح ولا لأ؟
+
+190
+00:14:15,310 --> 00:14:19,170
+الآن في وراها على طول في اسمه two biovar أو bio
+
+191
+00:14:19,170 --> 00:14:24,950
+type و biovar أو كلمة var VAR معناها type VAR جابني
+
+192
+00:14:24,950 --> 00:14:29,850
+ال variety variety معناها نوع فنوع أو وال type
+
+193
+00:14:29,850 --> 00:14:33,830
+نفس الشيء فمرة تقوله serotype أو serovar , biotype
+
+194
+00:14:33,830 --> 00:14:39,550
+أو biovar إذا نفس المعنى إحنا درسنا serotype و
+
+195
+00:14:39,550 --> 00:14:43,350
+درسنا phagotype صح اتعرفنا على البكتيريا من خلال
+
+196
+00:14:43,350 --> 00:14:47,570
+البكتيريوفاج فكنا إحنا phagotype الآن بنتكلم عن
+
+197
+00:14:47,570 --> 00:14:54,040
+حاجة اسمها biotype biotype ما معنى biotype Species
+
+198
+00:14:54,040 --> 00:14:57,440
+واحدة على سبيل المثال ال Vibrio cholera تركزوا معاها
+
+199
+00:14:57,440 --> 00:15:03,820
+عشان المفهوم واضح ما نندوش كتابة Species واحدة لجينا
+
+200
+00:15:03,820 --> 00:15:07,420
+الفرق الأساسي مش في الأنتيجينات وإنما في صفة
+
+201
+00:15:07,420 --> 00:15:09,580
+بيوكيميائية كونكوا دراستوا ال Voges Proskauer في
+
+202
+00:15:09,580 --> 00:15:14,380
+المعمل ال VP تخيلوا إنه في اثنتين من ال Vibrio cholera
+
+203
+00:15:14,380 --> 00:15:17,900
+اللي هي أوى اللي ممكن تكون إنابا أو ممكن تكون
+
+204
+00:15:17,900 --> 00:15:22,180
+أجاوة أو ممكن تكون هيكوجيما واحدة منهم positive
+
+205
+00:15:22,180 --> 00:15:25,720
+للفوجس بروسكاور وواحدة منهم negative للفوغس بروسكاور
+
+206
+00:15:25,720 --> 00:15:30,240
+كوار فالمتجرش يقول عنهم هدول serotype ولا يقول
+
+207
+00:15:30,240 --> 00:15:32,320
+عنهم Fasciotype إيش يقول عنهم اختلفوا في
+
+208
+00:15:32,320 --> 00:15:34,900
+biochemical characteristics أو physical
+
+209
+00:15:34,900 --> 00:15:38,260
+characteristics نسميهم biotypes .. biotypes فهي
+
+210
+00:15:38,260 --> 00:15:41,000
+معنى ال biotype في واحد اسمه classical أو classic
+
+211
+00:15:41,000 --> 00:15:45,240
+والثانية ال تور .. ال تور واحدة classic واحدة ال
+
+212
+00:15:45,240 --> 00:15:48,140
+تور طبعا ال classic المفهوم إن هي ال original هي
+
+213
+00:15:48,140 --> 00:15:49,840
+القديمة المعروفة سابقة
+
+214
+00:15:52,390 --> 00:16:00,150
+هذا ال diagram بيوضح
+
+215
+00:16:00,150 --> 00:16:03,750
+إن التقسيمة تبع ال vibrio cholera based on o-antigen
+
+216
+00:16:03,750 --> 00:16:08,010
+يكون فيه اثنين two o-antigens extracts فقط وهما 139 وهذا
+
+217
+00:16:08,010 --> 00:16:11,490
+يعني كمان أهميته مش كتير عالية، الأهمية لمين؟ لل
+
+218
+00:16:11,490 --> 00:16:16,280
+o, one الـ O1 فيه منه two biotypes اللي هم ال تور
+
+219
+00:16:16,280 --> 00:16:18,860
+وال classical اللي حكينا عنهم والفرق الأساسي
+
+220
+00:16:18,860 --> 00:16:21,640
+بينهم هو ال volume و ال score وفيه فروقات أخرى
+
+221
+00:16:21,640 --> 00:16:26,880
+هدوسها لاحقا كل واحد من الاثنين هدول قد يشتمل على
+
+222
+00:16:26,880 --> 00:16:32,020
+إنابا أو أجاوة أو هيكوجيما طبعا أنا بأذكر بس قصة
+
+223
+00:16:32,020 --> 00:16:37,030
+ال هيكوجيما كان فينا سؤالتين معروفين لما الإنابا وال
+
+224
+00:16:37,030 --> 00:16:40,050
+إنابا كيف كنا نتعرف عليها؟ كان في antiserum اسمه
+
+225
+00:16:40,050 --> 00:16:44,370
+anti-inaba نحطه على البكتيريا إذا تفاعل معها فهي
+
+226
+00:16:44,370 --> 00:16:48,890
+إنابا وفي antiserum اسمه أجاوة إذا حطيناه على
+
+227
+00:16:48,890 --> 00:16:52,310
+البكتيريا تفاعل معها لكن ال anti-inaba ما بتفعلش
+
+228
+00:16:52,310 --> 00:16:55,190
+مع الأجاوة والأجاوة ما بتفعل مع مين؟ مع الإنابا
+
+229
+00:16:55,940 --> 00:16:59,260
+يعني كل واحد بالفعل سلالة بنفصلها، يوم عزلناها، مش
+
+230
+00:16:59,260 --> 00:17:03,600
+إحنا العلماء اللي بيشتغلوا عزلوا بكتيريا، حطوا
+
+231
+00:17:03,600 --> 00:17:07,340
+عليها anti-inaba antiserum، حطوا عليها anti-inaba antiserum،
+
+232
+00:17:07,340 --> 00:17:10,780
+فصارت هي antiserum لمين؟ للإنابا والأجاوة، هذي معناها
+
+233
+00:17:10,780 --> 00:17:14,100
+حاجة إنه بتتكلم عن سلالة جديدة، طبعا تأكدوا، قعدوا
+
+234
+00:17:14,100 --> 00:17:16,280
+الفحص مرة ومرة وتكراره، انفعلوا طلعت سلالة جديدة و
+
+235
+00:17:16,280 --> 00:17:20,680
+سموها Hikoshi، تمام؟ إذا الإنابا وأجاوة وهيكوجيما
+
+236
+00:17:20,680 --> 00:17:24,160
+هدول عبارة عن sero types قدرلهم في ال classical أو
+
+237
+00:17:24,160 --> 00:17:27,620
+قدرلهم في ال تور وال تور وال classical هم bio
+
+238
+00:17:27,620 --> 00:17:34,080
+types لمين؟ لل O1 من sero type اثنين فقط من
+
+239
+00:17:34,080 --> 00:17:37,580
+ال vibrio ال O1 وال O139
+
+240
+00:17:39,950 --> 00:17:43,590
+ال fecal oral transmission هي ال route المشهودة
+
+241
+00:17:43,590 --> 00:17:48,070
+المعروفة في انتقال البكتيريا من ال feces اللي بتصل
+
+242
+00:17:48,070 --> 00:17:51,130
+ال water سواء كان fresh وال fresh مياه عذبة يعني
+
+243
+00:17:51,130 --> 00:17:54,810
+المياه الحلوة تمام؟ وال salt water اللي هي ال
+
+244
+00:17:54,810 --> 00:17:59,010
+marine water اللي زي مياه البحر لوّثت الغذاء لوّثت
+
+245
+00:17:59,010 --> 00:18:04,850
+المادة هنا تمام؟ هي طريقة الانتقال في مصطلحين
+
+246
+00:18:04,850 --> 00:18:08,460
+قدامنا هنا ال epidemic وال endemic ممكن ال endemic
+
+247
+00:18:08,460 --> 00:18:11,100
+أول مرة يقول معانا ولا مرّ معانا قبل هيك؟ ما مرّ
+
+248
+00:18:11,100 --> 00:18:15,100
+معانا endemic طيب نميز بين المصطلحين ونعرف إيش
+
+249
+00:18:15,100 --> 00:18:17,960
+أساسا Epidemic معناها كتير من الناس ترجمة باللغة
+
+250
+00:18:17,960 --> 00:18:20,100
+العربية Epidemic معناها وباء ترجمة باللغة 
+
+251
+00:18:20,100 --> 00:18:23,820
+العربية معناها واباة لكن مفهوم الوباء عند كثير من
+
+252
+00:18:23,820 --> 00:18:29,540
+الناس مفهوم خاطئ بنصحح اليوم once and for all مرة 
+
+253
+00:18:29,540 --> 00:18:35,390
+واحدة للأبد ما معنى واباة الوباء هو عبارة عن
+
+254
+00:18:35,390 --> 00:18:41,030
+increase in number of cases for certain disease عن 
+
+255
+00:18:41,030 --> 00:18:45,810
+مثيلاتها في السنوات السابقة كيف يعني؟ تركزوا معايا
+
+256
+00:18:45,810 --> 00:18:49,790
+عشان تفهموها والله أحسن من الكتابة احنا في شهر
+
+257
+00:18:49,790 --> 00:18:54,770
+إيش؟ احنا ديسمبر 2014 بنفرض أن احنا في أي أسبوع؟
+
+258
+00:18:54,770 --> 00:18:57,970
+الأسبوع الثاني؟ ولا لسه الأسبوع الأول؟ كده إيش
+
+259
+00:18:57,970 --> 00:19:03,330
+تاريخ اليوم؟ يعني بدأنا في الأسبوع الثاني لو جينا
+
+260
+00:19:03,330 --> 00:19:07,090
+عملنا إحصائيات عن مرض الـ influenza في فلسطين في
+
+261
+00:19:07,090 --> 00:19:11,890
+الأسبوع الأول من شهر ديسمبر عام 2014 لجينا 5000
+
+262
+00:19:11,890 --> 00:19:16,510
+حالة جينا قارنا مع السنة الماضية اللي هي 2013 و
+
+263
+00:19:16,510 --> 00:19:23,870
+2012 لجينا أن طول الشهر تبع ديسمبر في 2013 لما
+
+264
+00:19:23,870 --> 00:19:27,270
+دلوقتي الحالات أصبحت عالية بقدر 500 يبقى في الأسبوع
+
+265
+00:19:27,270 --> 00:19:32,610
+الأول الشهر اتضاعف في 2014 أضعاف مضاعفة عن
+
+266
+00:19:32,610 --> 00:19:36,790
+مين؟ عن كل الشهر تبقى هذا بنسميه هنا epidemic
+
+267
+00:19:36,790 --> 00:19:42,230
+طبعا؟ أما المفهوم اللي كان في أنكو سابقا عن الوباء
+
+268
+00:19:42,230 --> 00:19:46,450
+اسمه outbreak  هو انتشار مفاجئ .. هو
+
+269
+00:19:46,450 --> 00:19:50,410
+انتشار مفاجئ لمرض معين يسموه outbreak طبعا؟
+
+270
+00:19:50,410 --> 00:19:54,630
+outbreak إذا الوباء وصل الصورة مش معناها نيجي نقلل
+
+271
+00:19:54,630 --> 00:19:59,880
+الـ endemic  بالعربي معناها مستوطن، مش مستوطن
+
+272
+00:19:59,880 --> 00:20:04,760
+إسرائيل، لأ مستوطن .. المرض مستوطن، بمعنى أنه 
+
+273
+00:20:04,760 --> 00:20:10,420
+بيحدث بنسب مستقرة في نفس المكان، يعني احنا عندما
+
+274
+00:20:10,420 --> 00:20:13,260
+.. احنا جونا .. متى صار عندنا كوليرا؟ في السابعة
+
+275
+00:20:13,260 --> 00:20:18,760
+والتسعين .. من سنة ألف وتسعمائة وسبعة وسبعين، بس جابله،
+
+276
+00:20:18,760 --> 00:20:22,020
+بعرفش كوليرا، ممكن يحدث عندنا كمان خمس سنوات أو
+
+277
+00:20:22,020 --> 00:20:26,000
+سنتين، كمان سنة، بس مش كل يوم بيحدث عندنا ما بنعزلش
+
+278
+00:20:26,000 --> 00:20:29,620
+كوليرا بشكل روتيني في غزة، طبعا، لكن اللي روحوا على
+
+279
+00:20:29,620 --> 00:20:33,120
+الهند أو باكستان أو الفلبين أو اندونسيا أو أو أي
+
+280
+00:20:33,120 --> 00:20:37,380
+مناطق زي بوليفيا، هنلاقي أنهم بيعزلوا بشكل روتيني
+
+281
+00:20:37,380 --> 00:20:42,180
+في غزة حالات بنسميها sporadic cases، ما نضحك أن
+
+282
+00:20:42,180 --> 00:20:48,190
+المرض مستوطن في تلك المنطقة قد يحدث وضع Epidemic في
+
+283
+00:20:48,190 --> 00:20:50,910
+نفس المنطقة اللي .. اللي ما يكونوا فيها إيه؟
+
+284
+00:20:50,910 --> 00:20:55,270
+Endemic بمعنى أن السنة عدد الحالات تبع الكوليرا
+
+285
+00:20:55,270 --> 00:20:59,430
+زاد على السنوات السابقة لكن في الآخر المرض إيه؟
+
+286
+00:20:59,430 --> 00:21:02,730
+Endemic .. المرض Endemic وصلت إيش الفرق بين
+
+287
+00:21:02,730 --> 00:21:08,390
+Epidemic و Endemic؟ هذه الخارطة العالمية اللي
+
+288
+00:21:08,390 --> 00:21:13,510
+موجودة أمامكم بتوضح لنا أن التركيز وين في أفريقيا
+
+289
+00:21:14,160 --> 00:21:20,660
+أفريقيا لأن poor sanitary conditions الـ source of
+
+290
+00:21:20,660 --> 00:21:24,640
+drinking water is scarce ندرة في مصادر مياه الشرب
+
+291
+00:21:24,640 --> 00:21:29,100
+تمام؟ وبالتالي بالفعل احتمالية الانتشار طلع العراق
+
+292
+00:21:29,100 --> 00:21:34,800
+البلد اللي كانت في يوم من الأيام من أقوى البلدان
+
+293
+00:21:34,800 --> 00:21:39,420
+العربية وأكثرها تطورا لأن صار فيها حروب وصار
+
+294
+00:21:39,420 --> 00:21:43,070
+فيها destruction مستوى المعيشة انخفض، مستوى الخدمات
+
+295
+00:21:43,070 --> 00:21:47,290
+انخفض، مستوى انتشار الكوليرا في العراق موجود الآن،
+
+296
+00:21:47,290 --> 00:21:50,250
+فاهم؟ وهاد الخارطة طلع .. إذا تطلع على الجزر
+
+297
+00:21:50,250 --> 00:21:55,170
+الصوفية اللي هنا، بتوع 2007 و2009 وكل ما كبرت
+
+298
+00:21:55,170 --> 00:21:58,430
+الدائرة، كل ما زاد يعني معناها لحكي عدد الحالات، يعني
+
+299
+00:21:58,430 --> 00:22:02,230
+حجم الدائرة هو تعبير عن عدد الحالات اللي هو الإصابة
+
+300
+00:22:02,230 --> 00:22:06,190
+طبعا تلاحظوا الهند .. الهند أو شبه القارة الهندية،
+
+301
+00:22:06,190 --> 00:22:09,250
+شوفوا أفغانستان أيضا، هنا في هنا .. في أمريكا
+
+302
+00:22:09,250 --> 00:22:13,820
+اللاتينية في هنابوليفيا، لكن تطلع أوروبا، تطلعوا 
+
+303
+00:22:13,820 --> 00:22:18,880
+أمريكا، شمالية، مافيش، تمام؟ آسيا هي المصدر
+
+304
+00:22:18,880 --> 00:22:21,820
+الأساسي كانت للكوليرا آسيا وأفريقيا، بس الآن
+
+305
+00:22:21,820 --> 00:22:24,720
+لاحظوا التركيز بالفعل وإن موجود في أفريقيا، في
+
+306
+00:22:24,720 --> 00:22:30,440
+أفريقيا مافيش حاجة protected من الكوليرا، اليوم
+
+307
+00:22:30,440 --> 00:22:34,080
+العالم كله يعتبر .. لما يوصفوه قرية صغيرة هو قرية
+
+308
+00:22:34,080 --> 00:22:38,130
+صغيرة، يعني مريض  حامل للميكروب بيروح لأمريكا لو
+
+309
+00:22:38,130 --> 00:22:40,510
+توفرت الظروف المناسبة ممكن يصير عندهم برضه
+
+310
+00:22:40,510 --> 00:22:44,190
+epidemic في أمريكا يعني فالأمر مش مقتصر لكن هي
+
+311
+00:22:44,190 --> 00:22:47,170
+موضوع توفر والظروف الملائمة اللي انتشرت على المرض
+
+312
+00:22:47,170 --> 00:22:51,650
+الـ pathogenesis  جولنا لازم الإنسان يصير يعمل
+
+313
+00:22:51,650 --> 00:22:55,490
+ingestion لـ 10 أس 8 لـ 10 أس 10 microorganism
+
+314
+00:22:55,490 --> 00:22:58,710
+يعني من 100 مليون لـ أكثر من حتى 1000 مليون 10 أس
+
+315
+00:22:58,710 --> 00:23:03,290
+9 لـ 1000 مليون Non-invasive infection of the small
+
+316
+00:23:03,290 --> 00:23:06,590
+intestine فقط ما بتروح للدم يعني لم نعزلها من
+
+317
+00:23:06,590 --> 00:23:09,570
+مكان آخر غير الـ intestinal tract يعني المكان اللي
+
+318
+00:23:09,570 --> 00:23:15,530
+بنعزل منه الـ Vibrosamine زي الشجلة شجلة لم نعزلها
+
+319
+00:23:15,530 --> 00:23:19,530
+من أماكن أخرى غير الـ intestinal tract Organism
+
+320
+00:23:19,530 --> 00:23:24,820
+secretes enterotoxin اللي هو الـ Exotoxin المعروف
+
+321
+00:23:24,820 --> 00:23:29,380
+بالـ Vibrio ودرستوا أنتو لما درسنا الـ pathogenesis و
+
+322
+00:23:29,380 --> 00:23:32,060
+الـ virulence factors ولما درسنا الـ Escherichia
+
+323
+00:23:32,060 --> 00:23:35,940
+coli أحد الأنواع سمينا enterotoxinic Escherichia
+
+324
+00:23:35,940 --> 00:23:41,360
+coli وقلنا it produces cholera-like toxin اللي هو
+
+325
+00:23:41,360 --> 00:23:48,160
+الـ L-T-Toxin الـ L-T تذكر الـ L-T-Toxin it acts
+
+326
+00:23:49,330 --> 00:23:52,830
+in a similar way to that of Vibrio cholera هذا
+
+327
+00:23:52,830 --> 00:23:55,650
+الكلام اللي حكيناه يعني بما أن احنا وصلنا لأصل الكلام
+
+328
+00:23:55,650 --> 00:23:59,030
+عن الـ Vibrio cholera هنتطلع على التكسين كيف بيشتغل
+
+329
+00:23:59,030 --> 00:24:02,990
+الآن لكن الـ result للتكسين اللي هو الـ water ايه ده
+
+330
+00:24:02,990 --> 00:24:07,850
+يعني بعض الـ virulence factors اللي اخترناها طبعا
+
+331
+00:24:07,850 --> 00:24:11,170
+مش هي كل مش كلها لكن قائمة مقتصرة منها اللي هي
+
+332
+00:24:11,170 --> 00:24:13,950
+الكوليرا توكسين اللي حكينا عنه والبيلاي وفي بعض
+
+333
+00:24:13,950 --> 00:24:16,890
+الـ adhesins الأخرى فاكرين المحاضرة السابقة تحدثت
+
+334
+00:24:16,890 --> 00:24:23,710
+أنا على fimbrial ومصطلح fimbrial ومصطلح fimbrial
+
+335
+00:24:23,710 --> 00:24:27,310
+ومصطلح fimbrial ومصطلح fimbrial ومصطلح fimbrial
+
+336
+00:24:27,310 --> 00:24:31,110
+ومصطلح fimbrial ومصطلح
+
+337
+00:24:31,110 --> 00:24:32,890
+fimbrial ومصطلح fimbrial ومصطلح fimbrial ومصطلح
+
+338
+00:24:32,890 --> 00:24:34,150
+fimbrial ومصطلح fimbrial ومصطلح fimbrial ومصطلح
+
+339
+00:24:34,150 --> 00:24:37,050
+fimbrial ومصطلح fimbrial ومصطلح fimbri
+
+340
+00:24:40,000 --> 00:24:42,640
+ماذا يعني بروتياز؟ بروتياز فاكرين أنتو قلتوا
+
+341
+00:24:42,640 --> 00:24:46,380
+هنسميه IgA بروتياز بس هذا .. هذا مختلف هذا مختلف
+
+342
+00:24:46,380 --> 00:24:50,940
+مش IgA بروتياز البروتياز
+
+343
+00:24:50,940 --> 00:24:54,200
+بشكل عام مصطلح لأنزيمات قادت على تكسير البروتينات
+
+344
+00:24:54,200 --> 00:24:58,920
+لما أقول أنهيم agglutination بروتياز بقولنا الوظيفة ..
+
+345
+00:24:58,920 --> 00:25:01,980
+الوظيفة تبعته لو كلمة agglutination يعني بقى الحاجة
+
+346
+00:25:01,980 --> 00:25:07,870
+أنه بيجمع الـ .. لكن بيساعد نفسه أنه to release the
+
+347
+00:25:07,870 --> 00:25:11,130
+bacteria from membranes from mucosal cells
+
+348
+00:25:11,130 --> 00:25:16,670
+الميوكوزا فيها ميوكاس أنا كيف بتتشبك البكتيريا
+
+349
+00:25:16,670 --> 00:25:22,830
+فهو بيسلكها من هذا الميوكاس لبروتين أو لبروتياز الـ
+
+350
+00:25:22,830 --> 00:25:24,530
+hemagglutination اللي هو جثة الـ hemagglutination
+
+351
+00:25:24,530 --> 00:25:28,790
+معناه أنه عبارة عن بروتين قادر لو أنا حاططه على
+
+352
+00:25:28,790 --> 00:25:31,390
+blood cell يجمع blood cell مع بعض بعمله
+
+353
+00:25:31,390 --> 00:25:33,690
+agglutination بيعمل لهم agglutination، بيسموه
+
+354
+00:25:33,690 --> 00:25:37,850
+hemagglutination، وهذه الصفة بنميز فيها الـ
+
+355
+00:25:37,850 --> 00:25:40,970
+classical عن الـ El Tor، عشان أقول أن الفوكس
+
+356
+00:25:40,970 --> 00:25:43,410
+بروكساكور، وهي الـ hemagglutination كمان بنميزهم
+
+357
+00:25:43,410 --> 00:25:47,270
+فيها، لفلجيلوم طبعا organ of motility، وأنا مئات
+
+358
+00:25:47,270 --> 00:25:51,550
+المرات ذكرت، قلت أن الـ motility is a virulence
+
+359
+00:25:51,550 --> 00:25:55,210
+factor، لأنها بتبعدنا عن الخطر وبتقربنا من اللي 
+
+360
+00:25:55,210 --> 00:25:59,020
+هو الغداء سيدلوف واروس بتعمل iron sequestration
+
+361
+00:25:59,020 --> 00:26:02,180
+وهذا أمر قلنا مطلوب جدا اللي هي الـ iron capturing
+
+362
+00:26:02,180 --> 00:26:08,060
+اللي هي التقاط الـ iron من ومنافسة الـ host عليه الـ
+
+363
+00:26:08,060 --> 00:26:13,000
+كودراتوكسين أنا لما بحط لكم هنا link أنا بحكي أنه 
+
+364
+00:26:13,000 --> 00:26:15,560
+لو ضغطوا على الـ link هذا بتروح على اليوتيوب إن شاء
+
+365
+00:26:15,560 --> 00:26:18,300
+الله عشان تشوفوا كيف التوكسين بيشتغل يعني بيكون في
+
+366
+00:26:18,300 --> 00:26:24,900
+فيديو توضيح لهذا الموضوع طيب it is encoded by a
+
+367
+00:26:24,900 --> 00:26:30,460
+bacteriophage يبقى التهمة سقطت عن مين؟ يعني غير محاسبة
+
+368
+00:26:30,460 --> 00:26:35,660
+البكتيريا على أفعالها لأن هي اللي بيقوم بالمهمة
+
+369
+00:26:35,660 --> 00:26:39,680
+القادرة على هذه البكتيريا هو الـ bacteriophage اللي
+
+370
+00:26:39,680 --> 00:26:42,800
+بيعطيها المقدرة على إنتاج التوكسين خلاص براءة الـ
+
+371
+00:26:42,800 --> 00:26:47,200
+Bible كلنا اليوم الـ molecular mass للتوكسين هو
+
+372
+00:26:47,200 --> 00:26:53,300
+عبارة عن 84 ألف دالتون و بيتكون من two sub units
+
+373
+00:26:53,300 --> 00:26:58,030
+فاكرينهم الـ A sub unit والـ B sub unit الـ A اللي
+
+374
+00:26:58,030 --> 00:27:00,270
+هي الـ active اللي هي الـ toxic والـ B اللي هي الـ
+
+375
+00:27:00,270 --> 00:27:01,910
+binding تعالى نطلع على الـ binding، بيقولنا الـ 
+
+376
+00:27:01,910 --> 00:27:05,670
+binding the receptor استبعته هي ال GM1-Ganglioside
+
+377
+00:27:05,670 --> 00:27:08,070
+اللي موجودة على ال enterocytes ال enterocytes دي
+
+378
+00:27:08,070 --> 00:27:11,450
+هي الخلايا اللي موجودة في ال intestinal tract فيها
+
+379
+00:27:11,450 --> 00:27:16,950
+GM Protein أو بيسموه GM1-Ganglioside Protein ال 
+380
+00:27:16,950 --> 00:27:21,330
+-subunit ترتبط فيه هذا معناه حكي أنه التكسين بيدخل
+
+381
+00:27:21,330 --> 00:27:24,550
+في هذه الخلايا اللي هي اسمها enterocytes إيش بيعمل
+
+382
+00:27:24,550 --> 00:27:30,450
+التكسين؟بيعمل حاسم ADP-ribosylation تاكرين كلمة
+
+383
+00:27:30,450 --> 00:27:36,130
+ADP-ribosylation أنا ذكرتها في .. تمام مثلا ..
+
+384
+00:27:36,130 --> 00:27:42,610
+مثلا أنا .. مثلا أنا بدي .. بدي واضح نقطة هذا أن
+
+385
+00:27:42,610 --> 00:27:47,130
+ال ADP-ribosylation ممكن تكون عملية من عملية تانية
+
+386
+00:27:47,130 --> 00:27:50,270
+يما switch on يما switch off يعني يا activation يا
+
+387
+00:27:50,270 --> 00:27:56,810
+دي activation بتطلع زي واحدة بتغطي زرالكبسة أو
+
+388
+00:27:56,810 --> 00:28:00,150
+الضغط هي ال ADP-ribosylation كلامك صحيح ال ADP
+
+389
+00:28:00,150 --> 00:28:04,050
+-ribosylation بعمل activation لأحد المركبات اللي
+
+390
+00:28:04,050 --> 00:28:08,370
+بتنشط الأدنية الساكنية تنزعها فبينشط الأدنية
+
+391
+00:28:08,370 --> 00:28:12,350
+الساكنية تنزعها إذا نشط بيتحول في النشاط هذا من ال
+
+392
+00:28:12,350 --> 00:28:16,690
+ATP ال ATP لمين؟ لساكنية EMP وبالتالي
+
+393
+00:28:19,560 --> 00:28:24,020
+اللملة الأخيرة المشهورة اللي صار عنها دايما انفسر
+
+394
+00:28:24,020 --> 00:28:26,260
+فيها اللي هو ال accumulation of electrolytes اللي
+
+395
+00:28:26,260 --> 00:28:29,360
+diluted بيه اللي هو كميات كبيرة أو copious amount
+
+396
+00:28:29,360 --> 00:28:34,100
+of water ال
+
+397
+00:28:34,100 --> 00:28:38,020
+vibrocholera clinical manifestation بقولنا may
+
+398
+00:28:38,020 --> 00:28:41,100
+range from asymptomatic colonization to fatal
+
+399
+00:28:41,100 --> 00:28:44,940
+diarrhea يبقى فيه خشية من ال asymptomatic
+
+400
+00:28:44,940 --> 00:28:47,140
+colonization اللي سميناها carrier
+
+401
+00:28:50,600 --> 00:28:54,520
+يعني واحد career و لا يبدو عليه اي علامات انه مصاب
+
+402
+00:28:54,520 --> 00:28:58,880
+لكن هو بيعمل shedding بيفرز الكوليرا في ال stool
+
+403
+00:28:58,880 --> 00:29:04,660
+تبقى وبالتالي هو قد يشكل مصدر عدو لمين للاخرين ال
+
+404
+00:29:04,660 --> 00:29:07,840
+onset يعني النوبة بتحدث من اتنين لتلت أيام after
+
+405
+00:29:07,840 --> 00:29:11,020
+ingestion يعني incubation period قصيرة جدا من
+
+406
+00:29:11,020 --> 00:29:14,740
+اتنين لتلت أيام لحاجة للبكتيريا عند المقدرة تكاتب
+
+407
+00:29:14,740 --> 00:29:21,000
+شكل مهول في ال intestinal tractupropt يعني مفاجئ
+
+408
+00:29:21,000 --> 00:29:27,300
+onset of water diarrhea and vomiting في فيديو
+
+409
+00:29:27,300 --> 00:29:31,080
+حاطولكوا يعني مش عارف في مكاننا عن educational
+
+410
+00:29:31,080 --> 00:29:37,200
+فيديو بالكارتون ووضح لنا كيف أن طفل أبوه أصيب
+
+411
+00:29:37,200 --> 00:29:42,780
+بالكلرة و ب simple procedures ب simple توعية في
+
+412
+00:29:42,780 --> 00:29:49,860
+المجتمع تبعه قضى على الكلرة الفيديو حلو طلع عليههو
+
+413
+00:29:49,860 --> 00:29:52,360
+حاجة بس عين كيك هو يمكن يكون في كتير water دياريا
+
+414
+00:29:52,360 --> 00:29:55,360
+و vomiting في البيت بس تحذير يا عمينا عملكوا
+
+415
+00:29:55,360 --> 00:29:59,220
+warning rice water stool rice water stool يقولنا
+
+416
+00:29:59,220 --> 00:30:02,780
+هدى characteristic للسطول للمرضى اللى عندهم
+
+417
+00:30:02,780 --> 00:30:07,880
+cholera ايش هدا rice water stool يعني شكل وقوان ال
+
+418
+00:30:07,880 --> 00:30:08,800
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+419
+00:30:08,800 --> 00:30:09,700
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+420
+00:30:09,700 --> 00:30:11,840
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+421
+00:30:11,840 --> 00:30:12,320
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+422
+00:30:12,320 --> 00:30:12,520
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+423
+00:30:12,520 --> 00:30:12,800
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+424
+00:30:12,800 --> 00:30:12,860
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+425
+00:30:12,860 --> 00:30:19,680
+ال .. ال .. ال .. ال .. ال .. ال .. الsevere fluid
+
+426
+00:30:19,680 --> 00:30:24,720
+and electrolyte loss أو loss بيقدر ال dehydration،
+
+427
+00:30:24,720 --> 00:30:27,960
+metabolic acidosis، hypervolemic shock،
+
+428
+00:30:27,960 --> 00:30:31,840
+hypervolemic shock، لأن المي خلاص، الدم بصير thick
+
+429
+00:30:31,840 --> 00:30:36,300
+أصلا، تمام؟ و Renal failure، لأن ال Renal failure
+
+430
+00:30:36,300 --> 00:30:40,730
+ممكن يحدث من combination، من اللي هوالـ water loss
+
+431
+00:30:40,730 --> 00:30:43,010
+و ال electrolyte imbalance عدم اتزان في ال
+
+432
+00:30:43,010 --> 00:30:48,470
+electrolyte تخيل يا بنات نسبة الوفيات 60% if
+
+433
+00:30:48,470 --> 00:30:54,110
+untreated one percent if treated يعني خفضنا ..
+
+434
+00:30:54,110 --> 00:30:57,190
+خفضنا .. النسبة الوفيات بدون انتباه تفقد بالك
+
+435
+00:30:57,190 --> 00:31:03,810
+نتكلم عليا ساهمين على اللي هو فقط تعويض السوائل و
+
+436
+00:31:03,810 --> 00:31:07,520
+ال .. و نعنهم ال electrolyte وبالتاليالحاجة اللى
+
+437
+00:31:07,520 --> 00:31:12,320
+قلتلكوا ب simple instructions منعنا المرض و في نفس
+
+438
+00:31:12,320 --> 00:31:15,200
+الوقت ب simple procedures ممكن نعالج كمان ايه
+
+439
+00:31:15,200 --> 00:31:18,540
+المرض لذلك التواعي انا دايما بقول التواعي يجب ان
+
+440
+00:31:18,540 --> 00:31:22,260
+يستثمر فيها في كل المجتمع يجب ان يستثمر في التواعي
+
+441
+00:31:22,260 --> 00:31:26,000
+و التواعي الحقيقية المبنية على معلومات من مصادر
+
+442
+00:31:26,000 --> 00:31:31,200
+موثوقة و ليس من مصادر من ال internet بتعطيكم مثال
+
+443
+00:31:31,200 --> 00:31:35,380
+على مصدر غير موثوق فاكرين صرا ان في influenza ال
+
+444
+00:31:35,380 --> 00:31:38,590
+..الطيور او الخنازير الله ماتحق بالظبط لإن هو
+
+445
+00:31:38,590 --> 00:31:41,870
+الأخطر .. الأخطر كانت الطيور و هي على فكرة في
+
+446
+00:31:41,870 --> 00:31:46,330
+حالات تجينا من مصر وفيات ب influence الطيور لإن
+
+447
+00:31:46,330 --> 00:31:48,830
+اقولكوا ال influence الطيور أخطر بكثير من ال
+
+448
+00:31:48,830 --> 00:31:54,170
+influence الأخر كانت فكان في حد هكذا بعد ال
+
+449
+00:31:54,170 --> 00:31:57,850
+internet انه to protect yourself اشرب يانسون،
+
+450
+00:31:57,850 --> 00:31:59,250
+اليانسون فقد من البلد
+
+451
+00:32:02,600 --> 00:32:06,820
+في واحد كتب قرأ برضه حاجة موظف عنده في الجامعة
+
+452
+00:32:06,820 --> 00:32:11,760
+استخدمت مادة كيميائية مش فاكر بالظبط شو هي بس يعني
+
+453
+00:32:11,760 --> 00:32:15,580
+دوّبها في مياه و اعطاها لأبناء اطفال بناء على
+
+454
+00:32:15,580 --> 00:32:19,260
+معلومات من وين و هو موظف و هو متعلم يعني متعلم مش
+
+455
+00:32:19,260 --> 00:32:23,160
+في مجالنا مش في مجال العلوم الطبية ولكن في الآخر
+
+456
+00:32:23,160 --> 00:32:30,210
+استقاء المعلومات من مصادر موثوقة او منيعني أهم من
+
+457
+00:32:30,210 --> 00:32:32,850
+المعلومة نفسها، صح ولا لأ؟ تخيل .. تخيل إنه ناخد
+
+458
+00:32:32,850 --> 00:32:35,970
+المعلومة مهما كانت روايتها لكن في الآخر تطلع غلط
+
+459
+00:32:35,970 --> 00:32:41,630
+وممكن تكون مضرة وبالتالي لأ مطلوب مننا أنه نكون
+
+460
+00:32:41,630 --> 00:32:48,510
+يعني حذرين ما ننقل لبعض معلومات دون التوفق منها زي
+
+461
+00:32:48,510 --> 00:32:51,950
+حكاية العلاج بالأدوية بال antibiotics واحد بيقول
+
+462
+00:32:51,950 --> 00:32:54,510
+للتاني بيقول له والله أنا عندي مثلا احتقان في
+
+463
+00:32:54,510 --> 00:32:58,080
+الظهر وكده قبلهاقولوله أنا ماتجيب الفترة في نفس
+
+464
+00:32:58,080 --> 00:33:02,860
+الظروف و الدكتور وصفلي أنت بقتك حب زرجة و خضرة و
+
+465
+00:33:02,860 --> 00:33:07,100
+أخدت نص ال course و شوفيت .. و نص ال course بتاعي
+
+466
+00:33:07,100 --> 00:33:09,540
+ضايق المولد عندى، دي بليا، و كل واحد دي .. دي
+
+467
+00:33:09,540 --> 00:33:16,120
+بليا، المهم انه بيقول انه الوعي .. الوعي مهم كتير
+
+468
+00:33:16,680 --> 00:33:21,340
+في ارتقاء المجتمعات وبالتالي احنا عندنا بعض العدد
+
+469
+00:33:21,340 --> 00:33:23,980
+.. انا بقولش العدد كلها .. بعض العدد السيئة في هذا
+
+470
+00:33:23,980 --> 00:33:28,340
+المجموع يعني ان احنا كناس مثقفين ومتعلمين .. انه
+
+471
+00:33:28,340 --> 00:33:33,660
+ننشر ثقافة .. انه لأ في جهات
+
+472
+00:33:33,660 --> 00:33:37,420
+مرجعية للاشياء اللي متعلقة بالطب .. انا لما بدروح
+
+473
+00:33:37,420 --> 00:33:40,560
+استفتي في مسألة دينية .. بقول هنا الشيخ .. باستفتي
+
+474
+00:33:40,560 --> 00:33:43,440
+.. هنا مفتي الجهة .. باستفتي .. لما باستفتي شيء ب
+
+475
+00:33:43,440 --> 00:33:49,090
+.. ب .. ب .. بأمر بخص صحعشان بعض الأمور طبيعية لكن
+
+476
+00:33:49,090 --> 00:33:52,390
+مش طبيعية في النهاية لبس تفتي منى الطبيب كمان كان
+
+477
+00:33:52,390 --> 00:33:57,030
+ليه او لأولادي او لعيلتي لبس دايما نطلب الفتوى او
+
+478
+00:33:57,030 --> 00:34:02,230
+نطلب المعلومة من مصدرها الصحيح على الرغم من انفجار
+
+479
+00:34:02,230 --> 00:34:04,550
+المعلومات اللى متوفر لدينا على الشبكات الانترنت
+
+480
+00:34:04,550 --> 00:34:08,830
+اللى انه اكتب المصادر مضروبة مش صحيحة مش دقيقة
+
+481
+00:34:08,830 --> 00:34:12,890
+واحد بكتبانها بالظبط، بكتب، بحط، و للأسف احنا
+
+482
+00:34:12,890 --> 00:34:16,250
+مشهورين بال copy and paste، العالم العربي مشهورين،
+
+483
+00:34:16,250 --> 00:34:20,450
+بنلاقي نفس المعلومة على مئة منتدى، نفسها، منسوخة و
+
+484
+00:34:20,450 --> 00:34:24,170
+محطوطة، فمسكين المواطن بتطلع على المقال بجدها
+
+485
+00:34:24,170 --> 00:34:26,810
+انتقرها بيها مائة ألف مرة، فبقول الله يعني معلومة
+
+486
+00:34:26,810 --> 00:34:28,110
+حقيقية، مكتوبة في ال internet
+
+487
+00:34:31,620 --> 00:34:35,760
+تفقنا على أن التوعية مهمة جدا في عملية الخفض تبع
+
+488
+00:34:35,760 --> 00:34:41,200
+الوافيات باستخدام اللي هو المحاليل أو تعويق
+
+489
+00:34:41,200 --> 00:34:43,300
+الاسرائيل اللي بيفقدها المرض
+
+490
+00:34:46,100 --> 00:34:48,740
+شايفين الصورة هذه اللي .. اللي .. بيبدو انها من
+
+491
+00:34:48,740 --> 00:34:51,800
+جورنال فرنسي، تعرف الصورة اللي فوق هذا اللي طيروا
+
+492
+00:34:51,800 --> 00:34:56,400
+معاه عصايا و فيها منجل من تحت، هذا حصاد الأرواح،
+
+493
+00:34:56,400 --> 00:35:01,300
+هذا حصاد .. هذا .. هذا الشكل اللي هو ال death أو
+
+494
+00:35:01,300 --> 00:35:04,180
+اللي بيجلب الموت، اللي بيجلب الموت، ال cholera
+
+495
+00:35:04,180 --> 00:35:08,790
+كانت توصف بإن بيعدى شكلإنه ملك الموت جاي و معاه
+
+496
+00:35:08,790 --> 00:35:12,210
+اللي هو ال .. بتقطع الرؤوس هذي و يبمره على
+
+497
+00:35:12,210 --> 00:35:15,370
+المجتمعات اللي بيقطع .. بضرب فيهم اللي بيموت ..
+
+498
+00:35:15,370 --> 00:35:18,130
+موت اللي ميموتش .. ب .. ب .. طلعوا الصورة اللي على
+
+499
+00:35:18,130 --> 00:35:20,970
+الجانب تاني شوفوا ال .. dehydration شايفين إنتوا
+
+500
+00:35:20,970 --> 00:35:25,690
+الصورة هذه؟ شايفينها كويس إنتوا؟ في ال .. في ال .. 
+
+501
+00:35:25,690 --> 00:35:28,570
+في الـ .. في الـ .. إذا اطلعتوا على المحاضرة بعد التسجيل
+
+502
+00:35:28,570 --> 00:35:30,750
+تبعها هتشوفوا لأن هو .. هتشوفوها من الـ .. من الـ ..
+
+503
+00:35:30,750 --> 00:35:34,790
+من الكمبيوتر الجلد فاكرين في رمضان كيف كنت تعمل و
+
+504
+00:35:34,790 --> 00:35:38,070
+كيف صايمين و مش صايمين؟ تمام؟ طبعًا كلكم كنتوا
+
+505
+00:35:38,070 --> 00:35:44,970
+تكونوا صايمين، هي قد اللي راجف، من إيه؟ الجلد فلما
+
+506
+00:35:44,970 --> 00:35:49,330
+بيكون فيه جفاف غالبًا الجلد will withstand يعني
+
+507
+00:35:49,330 --> 00:35:54,930
+بيظل واقف بشكل لفترة أطول لكن لما بيكون الـ fluid 
+508
+00:35:54,930 --> 00:35:58,690
+فيه بشكل جيد على طول بيرجع normal زي ما كان واضح
+
+509
+00:35:58,690 --> 00:36:02,150
+من مسكة الطفل هنا أن الجلد تبعه أن هو مصاب بالـ
+
+510
+00:36:02,150 --> 00:36:05,290
+dehydration بالـ dehydration طبعًا الـ goods اللي
+
+511
+00:36:05,290 --> 00:36:07,730
+بحكي هنا الـ dehydration and death severe loss of
+
+512
+00:36:07,730 --> 00:36:13,330
+ions and الـ immunity strong immunity after recovery
+
+513
+00:36:13,330 --> 00:36:18,550
+و بتكون نتيجة عن اللي هو الـ soluble immunoglobulin
+
+514
+00:36:18,550 --> 00:36:22,390
+A يعني بتعطينا immunity جيدة أنا قلت strong
+
+515
+00:36:22,390 --> 00:36:26,070
+immunity لكن مش معناه تلحقنا long immunity برضه
+
+516
+00:36:26,070 --> 00:36:31,230
+كمان يعني لن تدوم أكثر من سنتين لن تدوم أكثر من
+
+517
+00:36:31,230 --> 00:36:38,720
+سنتين بحد أقصى الـ pathological diagnosis Spacements
+
+518
+00:36:38,720 --> 00:36:42,440
+Stool Vomitus الستول طبعًا اللي هو المعروف البراز
+
+519
+00:36:42,440 --> 00:36:46,660
+و الـ Vomitus اللي هو القيء Stained Smear مايله أي
+
+520
+00:36:46,660 --> 00:36:51,460
+قيمة إلا الحاجة واحدة Stained Smear من العينتين
+
+521
+00:36:51,460 --> 00:36:55,900
+نعمل Grams ثاني نعمل Grams ثاني إنه نشوف الـ curved
+
+522
+00:36:55,900 --> 00:36:59,560
+بس الـ lie نشوف الـ curved بس الـ lie و الـ curved بس
+
+523
+00:36:59,560 --> 00:37:04,320
+الـ lie وجودها لا يعني بالضرورة ولادة الكوليرا وعدم
+
+524
+00:37:04,320 --> 00:37:08,280
+وجودها أيضًا لا يعني بالضرورة إنها غير موجودة يبقى
+
+525
+00:37:08,280 --> 00:37:13,280
+معنا الحقيقية مش كتير لها فائدة كبيرة الصحيح لكن
+
+526
+00:37:13,280 --> 00:37:16,080
+كانوا يعملوه للاستئناس في فترة الأوبئة كانوا يعملوا
+
+527
+00:37:16,080 --> 00:37:21,060
+الاستئناس فقط لها الـ culture هي الأهم على الإطلاق
+
+528
+00:37:21,060 --> 00:37:25,280
+عشان نجي نتعرف على الـ microbe و نفحص مدى حساسيته
+
+529
+00:37:25,280 --> 00:37:29,340
+للـ antibiotics في بيتنا نزرعه و المزرعة فيه نواسط
+
+530
+00:37:29,340 --> 00:37:32,120
+اسمه الـ T-CBS إذا قلنا كوليرا
+
+531
+00:37:37,970 --> 00:37:42,590
+Cholera alkaline TCBS إيش الـ TCBS؟ Thyosulfate
+
+532
+00:37:42,590 --> 00:37:51,210
+اكتبوا الاسم Thyosulfate citrate citrate
+
+533
+00:37:51,210 --> 00:37:56,930
+by salt sucrose
+
+534
+00:37:56,930 --> 00:38:02,370
+هذا الاسم مش للـ salt للـ sucrose مين
+
+535
+00:38:02,370 --> 00:38:05,410
+يقعد ليها مرة ثانية؟
+
+536
+00:38:11,150 --> 00:38:17,490
+هذا برضه الوصفة تعتبر مثال على selective and
+
+537
+00:38:17,490 --> 00:38:22,470
+differential selective and differential كتير
+
+538
+00:38:22,470 --> 00:38:25,430
+مرة بعينة selective and differential دلوقتي مكونكي
+
+539
+00:38:25,430 --> 00:38:30,350
+مكونكي و الـ metal salt agar تمام وهي عندنا الـ TCBS
+
+540
+00:38:30,350 --> 00:38:34,870
+طبعًا أحد الصفات تبعته من الـ selective إنه alkaline
+
+541
+00:38:34,870 --> 00:38:41,820
+pH طب كيف صار .. كيف صار .. differential من
+
+542
+00:38:41,820 --> 00:38:46,240
+السكروز واضح من السكروز دلوقتي إيش السكروز بتقول
+
+543
+00:38:46,240 --> 00:38:51,680
+بيقولوا أن البكتيريا إذا خمرت السكروز ففيه عندنا
+
+544
+00:38:51,680 --> 00:38:57,540
+وسط أو a pH indicator اللي .. اللي .. اللي بيخلي
+
+545
+00:38:57,540 --> 00:39:03,100
+الوسط لونه أخضر بيخلي الوسط لونه أخضر الآن إيش الـ
+
+546
+00:39:03,100 --> 00:39:06,720
+.. إيش الـ اسم الـ .. الـ .. الـ indicator اللي في
+
+547
+00:39:06,720 --> 00:39:14,140
+السيطرة bromothymol blue علشانك
+
+548
+00:39:14,140 --> 00:39:19,120
+الـ citrate اتحولت من الأخضر إلى أزرق نفسه هو اللي
+
+549
+00:39:19,120 --> 00:39:23,620
+احنا بنستخدمه bromothymol blue ركزوا معي اللي أنا
+
+550
+00:39:23,620 --> 00:39:27,880
+أقوله نمت البكتيريا على الطبق هذا وعملت
+
+551
+00:39:27,880 --> 00:39:31,640
+fermentation للـ سكروز حياتي فايدة بيقعدش ولا
+
+552
+00:39:31,640 --> 00:39:37,440
+هنقفل؟ هنقفل اللون الأصلي اللون الأصلي اللي وطن أخضر
+
+553
+00:39:38,160 --> 00:39:41,640
+لاحظوا في الـ citrate لما طلع فوق صار alkaline صار
+
+554
+00:39:41,640 --> 00:39:48,040
+أزرق لكن لو نزل تحت بيصير أصفر بيصير أصفر تحلج
+
+555
+00:39:48,040 --> 00:39:53,440
+المستعمرات تبعنا تبع الـ CBS مستعمرات صفراء محاطة
+
+556
+00:39:53,440 --> 00:39:57,440
+بمنطقة صفراء as a result of sucrose fermentation
+
+557
+00:40:10,450 --> 00:40:16,330
+هذا الكلام على فكرة أنا قلته مستعمرة صفراء محاطة
+
+558
+00:40:16,330 --> 00:40:21,090
+بمنطقة صفراء as a result of Manitou fermentation
+
+559
+00:40:21,090 --> 00:40:25,590
+اختلف الـ media و أنا قلت لك الوصف دائمًا المستعمر
+
+560
+00:40:25,590 --> 00:40:29,610
+يبقى أن يكون مرتبط بمين؟ الوصف المستعمر مرتبط بنوع
+
+561
+00:40:29,610 --> 00:40:35,250
+الوسط نفسه طبعًا الـ .. الـ quick immunological method
+
+562
+00:40:35,250 --> 00:40:37,010
+أنا شرحته المرة الماضية اللي هو الـ
+
+563
+00:40:37,010 --> 00:40:39,430
+immunofluorescent و الـ immunofluorescent إنه أنا
+
+564
+00:40:39,430 --> 00:40:43,770
+أضع شريحة .. أنا أضع بكتيريا على شريحة و أعملها
+
+565
+00:40:43,770 --> 00:40:47,930
+fixation و أضيف عليها fluorescent antibody و بعدين
+
+566
+00:40:47,930 --> 00:40:51,690
+أغسل بعد الغسيل بنطلع على الـ fluorescent
+
+567
+00:40:51,690 --> 00:40:54,730
+microscopy الـ fluorescent microscopy عبارة عن
+
+568
+00:40:54,730 --> 00:40:58,150
+ultraviolet light تسقط على الشريحة فيه fluorescent
+
+569
+00:40:58,150 --> 00:41:02,610
+بيمتصها وبيشعلنا visible light نقدر نشوفه بالعين
+
+570
+00:41:02,610 --> 00:41:05,330
+تبعنا، إذا نجينا visible light يعني فيه
+
+571
+00:41:05,330 --> 00:41:07,950
+fluorescent ليش الـ fluorescent ضائل؟ لأنه فيه
+
+572
+00:41:07,950 --> 00:41:11,470
+antibody، ليش الـ antibody ضائل؟ لأنه فيه بكتيريا،
+
+573
+00:41:11,470 --> 00:41:15,360
+ما شفنا visible light، ما فيش fluorescent طب أنا حاطط
+
+574
+00:41:15,360 --> 00:41:18,100
+فلوريسن راح مع الغسيل، ليش راح مع الغسيل؟ لأنه
+
+575
+00:41:18,100 --> 00:41:21,780
+مرتبط بالبكتيريا، ما فيش بكتيريا البحث نجد، تقنية
+
+576
+00:41:21,780 --> 00:41:24,960
+كتير ممتازة وسهلة، لكن شوية expensive لأن الـ
+
+577
+00:41:24,960 --> 00:41:28,180
+immunofluorescent agent نفسها غالية، الـ microscope
+
+578
+00:41:28,180 --> 00:41:31,080
+نفسه يعني تكلفته حوالي من ثمان آلاف ليه عشان ألف
+
+579
+00:41:31,080 --> 00:41:33,920
+دولار حسب الجودة، ممكن يوصل لعشرين ألف دولار كمان
+
+580
+00:41:33,920 --> 00:41:40,290
+بعض الميكروسكوبات المزودة بحواسيب وكمبيوترات أعتقد
+
+581
+00:41:40,290 --> 00:41:43,470
+أن الـ Vibrio prevention and control improved
+
+582
+00:41:43,470 --> 00:41:46,670
+sanitation لازم نحسن النظافة العامة بشكل عام في
+
+583
+00:41:46,670 --> 00:41:49,730
+البلد fluid and electrolyte replacement أنا مكبرها
+
+584
+00:41:49,730 --> 00:41:52,990
+في خط كبير عشان أقول لكم أن جزء مهم جدًا من العلاج
+
+585
+00:41:52,990 --> 00:41:54,970
+و هذا الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ
+
+586
+00:41:54,970 --> 00:41:55,250
+.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ ..
+
+587
+00:41:55,250 --> 00:41:55,370
+الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ ..
+
+588
+00:41:55,370 --> 00:41:55,670
+.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ ..
+
+589
+00:41:55,670 --> 00:41:55,750
+الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ ..
+
+590
+00:41:55,750 --> 00:41:56,310
+.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ ..
+
+591
+00:41:56,310 --> 00:41:57,070
+الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ ..
+
+592
+00:41:57,070 --> 00:42:00,870
+.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ ..
+
+593
+00:42:00,870 --> 00:42:07,050
+الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ ..
+
+594
+00:42:08,430 --> 00:42:11,610
+كمان احنا كنا قبل شوية .. أي .. أي .. أي شيء متعلق
+
+595
+00:42:11,610 --> 00:42:14,310
+بنقله أهل الفكرة الأورال بدنا نشوف الـ food
+
+596
+00:42:14,310 --> 00:42:17,410
+handlers لأن الـ food handlers قد يكونوا carriers
+
+597
+00:42:17,410 --> 00:42:20,010
+.. قد يكونوا carriers لـ .. لـ .. لـ .. لـ .. لـ .. لـ
+
+598
+00:42:20,010 --> 00:42:20,170
+.. لـ .. لـ .. لـ .. لـ .. لـ .. لـ .. لـ .. لـ .. لـ ..
+
+599
+00:42:20,170 --> 00:42:20,410
+.. لـ .. لـ .. لـ .. لـ .. لـ .. لـ .. لـ .. لـ .. لـ ..
+
+600
+00:42:20,410 --> 00:42:21,110
+.. لـ .. لـ .. لـ .. لـ .. لـ .. لـ .. لـ .. لـ .. لـ ..
+
+601
+00:42:21,110 --> 00:42:21,990
+.. لـ .. لـ .. لـ .. لـ .. لـ .. لـ .. لـ .. لـ .. لـ ..
+
+602
+00:42:21,990 --> 00:42:23,230
+.. لـ .. لـ .. لـ .. لـ .. لـ .. لـ .. لـ .. لـ .. لـ ..
+
+603
+00:42:23,230 --> 00:42:24,550
+.. لـ .. لـ .. لـ 
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/G0WxTYLs1LE_postprocess.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/G0WxTYLs1LE_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..67bc84577d50b2a4e1ab722d25a5b3919f407f69
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/G0WxTYLs1LE_postprocess.srt
@@ -0,0 +1,2076 @@
+1
+00:00:20,750 --> 00:00:22,710
+الله الرحمن الرحيم الحمد لله رب العالمين أصلا
+
+2
+00:00:22,710 --> 00:00:25,170
+وأستدعم على سيدنا محمد وعلى أهلي وأصحابي ومن ساعة
+
+3
+00:00:25,170 --> 00:00:29,110
+وإدارة وفردين المابعد الموضوع اللي تبدأنا فيه
+
+4
+00:00:29,110 --> 00:00:34,350
+موضوع الصحيحة مهم وخطير لإنه بيشمل جزئية متعلقة ب
+
+5
+00:00:34,350 --> 00:00:37,410
+how to prevent infectious diseases اللي هي بعنوان
+
+6
+00:00:37,410 --> 00:00:43,490
+bacterial vaccines ال bacterial vaccines حاولنا
+
+7
+00:00:43,490 --> 00:00:48,030
+إنه نقسم العملية لتسمين رئيسيين active immunity و
+
+8
+00:00:48,030 --> 00:00:52,400
+passive immunityو ال active immunity تحدثنا عن
+
+9
+00:00:52,400 --> 00:00:57,320
+ثلاث أشكال من ال vaccination كنا أن دي ال capsule
+
+10
+00:00:57,320 --> 00:01:02,340
+polysaccharide أو الكابسول بشكل عام و اتكلمنا عن
+
+11
+00:01:02,340 --> 00:01:05,940
+ال bacterial holes اللي إما live أو attenuated
+
+12
+00:01:05,940 --> 00:01:10,360
+يعني إما ميتة أو مضعفة
+
+13
+00:01:17,250 --> 00:01:21,650
+أسماء اللي هم Structococcus pneumonia, Hemophilus
+
+14
+00:01:21,650 --> 00:01:27,050
+influenza واتكلمنا عن ناسيريا من الريبايت. بعدين
+
+15
+00:01:27,050 --> 00:01:31,850
+اتقلنا للتوكسويدس ولاجينا انه فينا DBT الدفتيريا
+
+16
+00:01:31,850 --> 00:01:36,270
+والبرتوزس والتيتانوس الدفتيريا مافي كان خلاف
+
+17
+00:01:36,270 --> 00:01:39,370
+والتيتانوس مافي خلاف كان خلاف على البرتوزس
+
+18
+00:01:39,370 --> 00:01:44,100
+واتحدثنا على انه البرتوزسلما فهمنا الـ mechanism
+
+19
+00:01:44,100 --> 00:01:47,740
+of pathogenesis عرفنا أنه ليس التكسين لحاله هو
+
+20
+00:01:47,740 --> 00:01:50,980
+المسؤول عن الـ pathogenesis لجدنا أنه كمان أدى
+
+21
+00:01:50,980 --> 00:01:54,220
+components زي الـ filamentous hemagglutinin و زي
+
+22
+00:01:54,220 --> 00:01:59,100
+البريتكتين و بالتالي مضطرين أنه نظيفة هناك معلومة
+
+23
+00:01:59,100 --> 00:02:05,100
+مهمة كمان بالاضافة إلى هذا في موضوع البرتروسيز وهو
+
+24
+00:02:05,100 --> 00:02:11,060
+أن الـ toxoid genetically inactivatedيعني ال
+
+25
+00:02:11,060 --> 00:02:15,020
+inactivation مش chemical وانما genetical بمعنى انه
+
+26
+00:02:15,020 --> 00:02:19,960
+تم ال .. ال .. ال .. عندها سبت الجين و .. وضعوا
+
+27
+00:02:19,960 --> 00:02:24,340
+فيه بكتيريا فأصبحت تنتج لنا toxoid مباشرة مش toxin
+
+28
+00:02:24,340 --> 00:02:28,640
+يعني inactivated وذلك عن طريق تغيير two amino
+
+29
+00:02:28,640 --> 00:02:33,500
+acids two amino acids تم تغييرهم وبالتالي أصبح لنا
+
+30
+00:02:33,500 --> 00:02:39,920
+toxoid احتفظ بال immunogenicity وفقد ال toxicity
+
+31
+00:02:45,120 --> 00:02:49,120
+واعتبر هذا التوكسويد هو the first genetically
+
+32
+00:02:49,120 --> 00:02:53,540
+engineered توكسويد و بتاعي دخل التاريخ من أنه the
+
+33
+00:02:53,540 --> 00:02:58,120
+first و ال vaccine هذا is indicated for every
+
+34
+00:02:58,120 --> 00:03:00,320
+child as protection against whooping cough،
+
+35
+00:03:00,320 --> 00:03:02,400
+whooping cough في نفس المعنى للبرتوسس اللي هي
+
+36
+00:03:02,400 --> 00:03:05,360
+السؤال ال common name يعني ال common name أو اسم
+
+37
+00:03:05,360 --> 00:03:10,950
+الشائع للبرتوسس اسمه whooping coughit is given
+
+38
+00:03:10,950 --> 00:03:15,090
+along with diphtheria and tetanus وكنا كهان قديم
+
+39
+00:03:15,090 --> 00:03:18,570
+اللي هو ال DPT وهو اللي لازم يستخدم في قطاع غزة
+
+40
+00:03:18,570 --> 00:03:22,150
+بيستخدم ال DPT بينما في أمريكا و في بعض البلدان
+
+41
+00:03:22,150 --> 00:03:25,770
+بدأ يستخدموا ال DTAP و ال AP اللي كنا كنا بيستخدمه
+
+42
+00:03:25,770 --> 00:03:29,530
+هو ال Acellular Fertosis اللي بيشمل التركسول و ال
+
+43
+00:03:29,530 --> 00:03:32,450
+hemagglutinin اللي حكينا عنه و ال p-retactin اللي
+
+44
+00:03:32,450 --> 00:03:38,710
+احنا كنا برضه كنا مهمين في البعثه انا الbacillus
+
+45
+00:03:38,710 --> 00:03:46,750
+anthracisvaccine الكل بيذكر الحرب والعدوان على
+
+46
+00:03:46,750 --> 00:03:53,370
+العراق وإنه كان الغرب يسوق للعالم بإن العراق
+
+47
+00:03:53,370 --> 00:03:56,330
+بيمتلك أسلحة بيولوجية ومن من الأسلحة البيولوجية
+
+48
+00:03:56,330 --> 00:03:59,470
+كان حدث عن ال anthrax إنه بيمتلك مئات الأطنان من
+
+49
+00:03:59,470 --> 00:04:03,670
+ال anthrax خلينا ناخد مقارنة صغيرة عن الحرب
+
+50
+00:04:03,670 --> 00:04:07,010
+البيولوجية عشان برضه نفهم ماذا يدور حولنا في
+
+51
+00:04:07,010 --> 00:04:11,120
+العالمأو على الأرض بقى يسموها biological warfare
+
+52
+00:04:11,120 --> 00:04:15,080
+باللغة الإنجليزية، اللي انا صار الكلام .. بلغة
+
+53
+00:04:15,080 --> 00:04:16,460
+أخرى صارت كلام عن bioterrorism
+
+54
+00:04:20,180 --> 00:04:25,440
+لأن بالأول كان مسموح للدول إنها تعمل أسلحة
+
+55
+00:04:25,440 --> 00:04:27,580
+بيولوجية و بعدين صار في اتفاقي في السبعينات من
+
+56
+00:04:27,580 --> 00:04:31,080
+القرن الماضي للحد أو لمنع انتشار الأسلحة
+
+57
+00:04:31,080 --> 00:04:34,860
+البيولوجية فصار أي استفدام ولا تباري إرهاب فسموه
+
+58
+00:04:34,860 --> 00:04:39,320
+bioterrorism و الحقيقة حتى يلثقوا بأهل الإسلام و
+
+59
+00:04:39,320 --> 00:04:43,740
+المسلمين فbioterrorism أسهل للإسلام فكان في نقطة
+
+60
+00:04:43,740 --> 00:04:50,050
+في أحد ال .. في .. بعد سبتمبر elevenتم توزيع
+
+61
+00:04:50,050 --> 00:04:55,190
+مغلفات بريدية على ناس وصلتهم فتحوها وطلع منها بودر
+
+62
+00:04:55,190 --> 00:04:58,570
+البودر هذه كانت بتحتوي على ال sports تبعت
+
+63
+00:04:58,570 --> 00:05:05,070
+الbacillus anthracis فطبعا انا بتحقيق مين المسئول
+
+64
+00:05:05,070 --> 00:05:11,190
+عن هذه الجريمة الكبرى اللي مات فيها ناس فكان فيه
+
+65
+00:05:11,190 --> 00:05:15,870
+كاريكاتير كتير معبر عما نوريد قوله واحد بتبقى تحت
+
+66
+00:05:15,870 --> 00:05:20,040
+ال microscope وبيحقق في الموضوعفبيقول هو الأمور
+
+67
+00:05:20,040 --> 00:05:24,400
+لسه مش واضحة، لكن الميكروف له ملامة شرق أوسطية،
+
+68
+00:05:24,400 --> 00:05:27,420
+للمؤشر يعني طول ما جاهز، بس يعني كيف بدنا نخرجها،
+
+69
+00:05:27,420 --> 00:05:29,860
+كيف بدنا نخرجها، فال .. فالميكروف ملامة شرق
+
+70
+00:05:29,860 --> 00:05:36,500
+أوسطية، وليزيانة حرب كتر، انه في حرب على الإرهاب
+
+71
+00:05:36,500 --> 00:05:41,540
+وحرب على العراق، انه في أسلحة بيولوجية عملوا تطييم
+
+72
+00:05:41,540 --> 00:05:45,140
+للجيش الأمريكي ال .. ال .. ال .. ضد الانتراكز
+
+73
+00:05:47,060 --> 00:05:49,700
+الاندراجستوكسين اللى بتتذكر هو بتكون من تلات قطعة،
+
+74
+00:05:49,700 --> 00:05:55,020
+تلات قطعة، A1 وA2 وB، B واحدة، الـB سمينها
+
+75
+00:05:55,020 --> 00:06:00,480
+Protective Antigen الـB هي القاعدة اللى من خلالها
+
+76
+00:06:00,480 --> 00:06:04,760
+برتبط A1 او A2 ومن ثم يصير فيه إدخال داخل الخلية،
+
+77
+00:06:04,760 --> 00:06:07,480
+وA1 اللى هو الاديمة الفاتر والتاني اللى يدعى
+
+78
+00:06:07,480 --> 00:06:11,410
+الفاترلو انا عملت antibodies ضد ال protective
+
+79
+00:06:11,410 --> 00:06:15,150
+antigen يبقى مناعة ال protective antigen انه يرتبط
+
+80
+00:06:15,150 --> 00:06:18,070
+بالخلال يعمل تنتراليزاجين وبالتالي لا A هيشتغل ولا
+
+81
+00:06:18,070 --> 00:06:22,210
+هيشتغل بيه وبالتالي بالفعل هنا عملنا immunization
+
+82
+00:06:22,210 --> 00:06:27,970
+لكن يبدو ان ال vaccine كان له side effects واتقن
+
+83
+00:06:27,970 --> 00:06:32,970
+ال vaccine بان هو اللي سبب ما يعرف حاليا ب Gulf
+
+84
+00:06:32,970 --> 00:06:33,670
+War Syndrome
+
+85
+00:06:41,580 --> 00:06:46,600
+بعض الجنود روحوه بعرض معينة وكان فيه theory ان ال
+
+86
+00:06:46,600 --> 00:06:49,500
+vaccine هو سبب هذه العرض ليس اتعرضهم لاي اشياء
+
+87
+00:06:49,500 --> 00:06:56,800
+اخرى في العرض انتقلنا من احنا من ال toxin ال
+
+88
+00:06:56,800 --> 00:07:00,260
+protein ال purified proteins الى ما يسمى ال killed
+
+89
+00:07:00,260 --> 00:07:04,420
+bacterial vaccines killed يعني الخلايا ميتةغير
+
+90
+00:07:04,420 --> 00:07:07,820
+قادر علي إنها تعمل multiplication نأمل إنه إذا
+
+91
+00:07:07,820 --> 00:07:12,000
+دخلت الجسم إنه الماكروفاج تاخدها و تفكفكها و تاخد
+
+92
+00:07:12,000 --> 00:07:15,540
+منها انتجنات تعملها representation و تخلي جهاز
+
+93
+00:07:15,540 --> 00:07:20,460
+المناعة ينجلها انت بطل تفيدني أربع عامة للصحية
+
+94
+00:07:20,460 --> 00:07:26,880
+هلونين و خاطرين Vibrio killer kill Yersinia pestis
+
+95
+00:07:26,880 --> 00:07:29,040
+Rickettsia Rickettsii اللي بسبب
+
+96
+00:07:32,870 --> 00:07:37,670
+وهو يذكركم بالبروتياس البروتياس اللى استخدمناها في
+
+97
+00:07:37,670 --> 00:07:42,070
+الكشف عن إبن كتسيا عن الـ typhus البروتياس ماهي
+
+98
+00:07:42,070 --> 00:07:45,410
+راح تقولناه في الـtyphus صح ولا لأ؟ طيب cross
+
+99
+00:07:45,410 --> 00:07:51,850
+reaction رقم أربع اللى هو ال Q-fever ماهي معناه ال
+
+100
+00:07:51,850 --> 00:07:59,980
+Q-fever؟ Q-fever حملة QQ جاي من كلمة Query Query
+
+101
+00:07:59,980 --> 00:08:03,400
+معناها Under Investigation يعني تحت التحقيق على
+
+102
+00:08:03,400 --> 00:08:07,020
+مستفاد Query لما اكتشفوا ال disease كان غير معروف
+
+103
+00:08:07,020 --> 00:08:11,360
+المسبب له فسمي Under Query Under Query Fever يعني
+
+104
+00:08:11,360 --> 00:08:15,380
+هم تحت التحقيق بدلا من نقول Under Query قصدنا نقول
+
+105
+00:08:15,380 --> 00:08:19,540
+Query Fever بعدها قصدنا نقول Q Fever فصار اسمه Q
+
+106
+00:08:19,540 --> 00:08:22,000
+Fever بعد ان اعرفه ان المسبب له اسمه Coxella
+
+107
+00:08:22,000 --> 00:08:26,440
+Bernetti لكن دل اسمه Q Fever لحد اليوم اسمه Q
+
+108
+00:08:29,420 --> 00:08:33,840
+الرقم تلاتة لمين بعتوه؟ بعتوه to immunize members
+
+109
+00:08:33,840 --> 00:08:37,320
+of the armed forces، القوات المسلحة، armed forces
+
+110
+00:08:37,320 --> 00:08:41,060
+معناه قوات المسلحة، ليه القوات المسلحة؟ فهنا ممكن
+
+111
+00:08:41,060 --> 00:08:44,440
+تتخيل عن طريق القمر والحشرات هناك، ال disease،
+
+112
+00:08:44,440 --> 00:08:50,640
+القوات المسلحة في الحروب ممكن تقضي أوقات طويلة
+
+113
+00:08:50,640 --> 00:08:57,280
+للناس بدون نظافة شخصية جيدةو يمكن .. يمكن سمعتهم
+
+114
+00:08:57,280 --> 00:09:00,780
+بقصص كثيرة أو فلان قعد بعد اشرين يوم، بعد تلاتين
+
+115
+00:09:00,780 --> 00:09:05,040
+يوم من الحرب، ف .. فبالتالي ال .. ال person و ال
+
+116
+00:09:05,040 --> 00:09:08,620
+regime بيكون ضعيف جدا، احتمالي انه طبعا بيكونوا ..
+
+117
+00:09:08,620 --> 00:09:12,480
+بيكونوش في اماكن مغلقة، بيكونوا في اماكن مكشوفة،
+
+118
+00:09:12,480 --> 00:09:15,640
+وبالتالي الحشرات موجودة، النعاق الموجودة، وبالتالي
+
+119
+00:09:15,640 --> 00:09:18,680
+to protect them احنا بنعطي هذا ال vaccine
+
+120
+00:09:20,000 --> 00:09:24,320
+اليرسينابستس كلكم بتعرفوا يعني الطعون المرض
+
+121
+00:09:24,320 --> 00:09:29,020
+التاريخي اللي ذُكر قبل القرآن حد في الأنجليزي
+
+122
+00:09:29,020 --> 00:09:33,180
+بيسموه بيبليكال ديزيز يعني مرض معروف قبل القرآن
+
+123
+00:09:33,180 --> 00:09:39,000
+موجود في الإنجيل والتوراق الطعون مرض خطير جدا لكن
+
+124
+00:09:39,000 --> 00:09:42,440
+بال vaccine could be prevented طبعا مش لو كنت
+
+125
+00:09:42,440 --> 00:09:45,820
+أناسي بيعطيه بيعطيه فقط للفئات اللي هم exposed
+
+126
+00:09:51,590 --> 00:09:55,950
+حتى ال Vibrio كوليرة و ال vaccine الأخير اللي هو
+
+127
+00:09:55,950 --> 00:10:00,370
+ال Q fever بنعطيه غالبا للناس اللي يلهم علاقة و
+
+128
+00:10:00,370 --> 00:10:04,010
+اهتكاك بالحيوانات لأنهم ينتقلون عن طريق الحيوانات
+
+129
+00:10:04,010 --> 00:10:10,050
+احفظنا منها 4 Vibrio, Carcinobestis, Coxella
+
+130
+00:10:10,050 --> 00:10:13,130
+Bernata و Ragetsia Ragetsii هدولة اللي سلم فيهم
+
+131
+00:10:13,130 --> 00:10:19,490
+killed بنا بكتيري نجيب للوز التالي من محاضرتنا
+
+132
+00:10:19,490 --> 00:10:22,540
+اللي هو ال passive immunityاتفقنا من ال passive
+
+133
+00:10:22,540 --> 00:10:28,240
+ايه بتتلخص ان انا بعطي preformed يعني مجهزة و
+
+134
+00:10:28,240 --> 00:10:32,360
+خالصة في لسه اما انسان او في لسه حياته ندبه لما
+
+135
+00:10:32,360 --> 00:10:37,820
+نتكلم على ليش في لسه انسان او في لسه حياته اشهر
+
+136
+00:10:37,820 --> 00:10:41,500
+مثال على ال passive immunity اللي هو ال tetanus
+
+137
+00:10:41,500 --> 00:10:44,940
+antitoxin tetanus antitoxin ده كان تعرف طول على
+
+138
+00:10:44,940 --> 00:10:51,560
+التعريف الأولاني antitoxinsبنجوسين هي عبارة عن
+
+139
+00:10:51,560 --> 00:10:55,060
+بنجوسين تانيهة في الحوادث انتيبادي أي مسمة سموها
+
+140
+00:10:55,060 --> 00:10:59,720
+انت سيراب انتيبادي انتي او immunoglobulins كلها في
+
+141
+00:10:59,720 --> 00:11:04,780
+الآخر هي فيها immunoglobulins يعني انتيبادي ضد
+
+142
+00:11:04,780 --> 00:11:09,880
+مين؟ غالبا ضد التوكسينز ليش ضد التوكسينز؟
+
+143
+00:11:09,880 --> 00:11:13,480
+التوكسينز هم موليكولات صغيرة في الغالب وهي ستتحول
+
+144
+00:11:21,850 --> 00:11:28,070
+قبل ما ترتبط خدوا بالكم التوكسين إذا ارتبطت غالبا
+
+145
+00:11:28,070 --> 00:11:33,890
+الأمر مش سهل فإحنا لما بنتدخل بال antitoxin غالبا
+
+146
+00:11:33,890 --> 00:11:38,310
+بنتدخل بحالة من حالتان إن ال prophylaxis و هذا
+
+147
+00:11:38,310 --> 00:11:42,910
+ممتاز جدا جدا جدا لإن التوكسين لم يسبقوش وصل الدم
+
+148
+00:11:42,910 --> 00:11:47,650
+و أنا جهازتله antibodies في الدم عطيهم للمريض عشان
+
+149
+00:11:47,650 --> 00:11:50,410
+لما التوكسين يبدأ ينتج على طول ال antiviral
+
+150
+00:11:50,410 --> 00:11:51,330
+neutralization
+
+151
+00:11:53,850 --> 00:11:56,390
+الحالة التانية ان اعطيه في ال treatment، ال
+
+152
+00:11:56,390 --> 00:12:00,630
+treatment مش معناها ان المرأة شغالة، موجودة، يعني
+
+153
+00:12:00,630 --> 00:12:04,150
+و المرض أصلا ناجي عن مين؟ عن ال toxin، و ال toxin
+
+154
+00:12:04,150 --> 00:12:09,070
+هو اللي سبب الأعراض، فالارتباط ال toxinبيكون صعب
+
+155
+00:12:09,070 --> 00:12:14,610
+اعمل neutralization بس انا فقط بمنع تدغور الحالة ب
+
+156
+00:12:14,610 --> 00:12:18,050
+new binding هبعمل neutralization لمين؟ لـ free
+
+157
+00:12:18,050 --> 00:12:22,470
+toxin مش لـ bound toxin وتفسورة يبقى برا بستخدمه ك
+
+158
+00:12:22,470 --> 00:12:25,850
+prevention هو فعال بيكون بينما في الحال ال
+
+159
+00:12:25,850 --> 00:12:31,290
+treatment ممكن مايكونش فعال كتير كما .. يعني انا
+
+160
+00:12:31,290 --> 00:12:35,530
+سبقت اللوحة ال .. اللوحة and in treatment and in
+
+161
+00:12:35,530 --> 00:12:40,630
+prevention و ذكرت مثلًاال antitoxin اللي بتعرض
+
+162
+00:12:40,630 --> 00:12:47,170
+للتيتانوس is made in human ممكن نلاقي بلد مين
+
+163
+00:12:47,170 --> 00:12:52,770
+يقبلوا أنهم يكونوا مصنع لل antibodies لل antitoxin
+
+164
+00:12:52,770 --> 00:12:56,990
+ده بالتأكيد لأ .. أيه اللي بيصير؟ الناس اللي ..
+
+165
+00:12:56,990 --> 00:13:00,620
+اللي .. اللي بتعرض للتيتانوسهو اللي بيسموهم hyper
+
+166
+00:13:00,620 --> 00:13:04,040
+immunized hyper ايش يعني hyper بالزيادة يعني عندهم
+
+167
+00:13:04,040 --> 00:13:08,040
+مستوى ال antibodies بيكون عالي جدا فبياخدوا منهم
+
+168
+00:13:08,040 --> 00:13:12,160
+من مجموعة و بيعملوا pooling ايش يعني pooling خدنا
+
+169
+00:13:12,160 --> 00:13:15,700
+منك و منها و منها و منها فصلنا ال serum و طبعا
+
+170
+00:13:15,700 --> 00:13:19,440
+اتأكدنا من سلامتهم من ان ماعندهم مش أمراض تنتقل
+
+171
+00:13:19,440 --> 00:13:24,480
+على طريق الدم زي ال HIV و ال HCV و ال HPV اللي هم
+
+172
+00:13:24,480 --> 00:13:28,240
+ال price B و ال C القاعدة تعملهم pooling pooling
+
+173
+00:13:28,240 --> 00:13:32,340
+يعنيبابدوا انه كان انت اخدنا منك عشرة سنطة، عشرين
+
+174
+00:13:32,340 --> 00:13:34,920
+سنطة، تلاتين سنطة، بابدوا انهم يكونوا كل واحد
+
+175
+00:13:34,920 --> 00:13:38,300
+لحالة، بنحطهم كلهم مع بعض، بيسموهم polyو بعدين
+
+176
+00:13:38,300 --> 00:13:42,640
+بنضمن ال stimuli بتبعتها عبر تقنيات معينة و بعدين
+
+177
+00:13:42,640 --> 00:13:48,560
+بوزهم في files و بيحطوهم في أماكن خاصة مش هلاجيهم
+
+178
+00:13:48,560 --> 00:13:53,020
+في كل عالم و في كل مستشفى لأنه ليش خدنا الإنسان
+
+179
+00:13:53,020 --> 00:13:55,360
+ماخدناش من الحيوانة، هممكن نصنعهم في الحيوانة؟
+
+180
+00:13:55,360 --> 00:13:58,240
+ممكن ما يناسكش الإنسان؟ في حالة اسمها hyper
+
+181
+00:13:58,240 --> 00:13:59,500
+sensitivity issues
+
+182
+00:14:02,300 --> 00:14:06,020
+وهي مرانا كمان شوية لو أخدتنا هي في نفس اللوحة لل
+
+183
+00:14:06,020 --> 00:14:10,580
+botulism toxin أخر إشي شفينها؟ وإن ان ال antitoxin
+
+184
+00:14:10,580 --> 00:14:14,920
+is made in horses so hypersensitivity may be a
+
+185
+00:14:14,920 --> 00:14:19,500
+problem عشان ما تنكرب ال hashبنتكلم عن فقه
+
+186
+00:14:19,500 --> 00:14:23,220
+الموازنات فقه الموازنات اللى .. اللى احنا بنفتقله
+
+187
+00:14:23,220 --> 00:14:27,720
+كثير من الأحيان و اللى بعض الأحيان بيخلّينا جامدين
+
+188
+00:14:27,720 --> 00:14:31,080
+التفكير بيخلّي .. بيخلّيناش انه .. انه نغير رأينا
+
+189
+00:14:31,080 --> 00:14:36,600
+ب .. ب .. بمرحلة ومرحلة الان انا عندي مريض صار
+
+190
+00:14:36,600 --> 00:14:41,600
+عنده على سبيل المثال جانجرين غرغرين يعني ال ..
+
+191
+00:14:41,600 --> 00:14:45,440
+الجانجرين في البداية هو خلق في المريض جرج الصولي و
+
+192
+00:14:45,440 --> 00:14:48,740
+أصابعيالشخص نفسه بيسمع من الجانجرينجة اللي قُلت له
+
+193
+00:14:48,740 --> 00:14:52,880
+صراحة احنا ممكن نحاول علاجها، نحاول علاجها، الان
+
+194
+00:14:52,880 --> 00:14:57,180
+بعد اسبوعين او تلاتة العملية مات و اربفت ولا عكس
+
+195
+00:14:57,180 --> 00:15:00,620
+زادت فالان احنا صارنا القراع دلوقت احنا .. احنا
+
+196
+00:15:00,620 --> 00:15:05,860
+كنا رفضين من الأول، اليوم وفدنا، هو ممكن يسأل سؤال
+
+197
+00:15:05,860 --> 00:15:08,820
+ويقول ليش ما قلتوا من الأول و رايحتوا حالكوا، ما
+
+198
+00:15:08,820 --> 00:15:13,000
+أنا قلتلكوا من الأولهذا الذكاء اللى قلنا عنه فى
+
+199
+00:15:13,000 --> 00:15:17,120
+فقه الموازنه ان الانسان بيتطلع بيشوف عنده ال
+
+200
+00:15:17,120 --> 00:15:21,340
+options الخيارات لان قطع اليد اكيد امر صعب على
+
+201
+00:15:21,340 --> 00:15:24,040
+الانسان ولا يقلق ولا يصراك ولا ادفر حتى لو واحد
+
+202
+00:15:24,040 --> 00:15:30,390
+اللى بيقصه و بياخد زيابه بيزعللكن في الآخر أنه
+
+203
+00:15:30,390 --> 00:15:36,470
+أسهل، أنه يعني خدنا نقول أفضل، مش أسهل، أنه أفضل،
+
+204
+00:15:36,470 --> 00:15:40,610
+إنه الحياة كلها تهلك، حياة الإنسان تهلك و لا يلقى
+
+205
+00:15:40,610 --> 00:15:45,150
+جذر منه، هالكل أخدته القرار إنه جذور، تمام؟أنا
+
+206
+00:15:45,150 --> 00:15:48,950
+كطبيب متى أخد هذا القرار عندي؟ أنا عندي معرفة أكتر
+
+207
+00:15:48,950 --> 00:15:53,930
+من المال نفسه وبالتالي بدنا نفهم أنه اللي عنده
+
+208
+00:15:53,930 --> 00:15:56,970
+معرفة كمان مرة و أنا بأكد عليه أكتر من مرة اللي
+
+209
+00:15:56,970 --> 00:16:00,710
+عنده معرفة أكتر بيكون قراره يعني مبني على نجم
+
+210
+00:16:00,710 --> 00:16:04,050
+الأفضل مبني على قاعدة أوسع من المال وبالتالي يمكن
+
+211
+00:16:04,050 --> 00:16:09,410
+يكون أسلسل أو أفضل القرار فالـ Hypersensitivity
+
+212
+00:16:09,410 --> 00:16:14,690
+بالمقارنة مع Botulismأقوى تكسين معروف للبشر على
+
+213
+00:16:14,690 --> 00:16:21,530
+كوكب الأرض Hypersensitivity و لا Botulism لو
+
+214
+00:16:21,530 --> 00:16:25,690
+تركناها هيموت تمام؟ و لو عقلناها هيحصلها انها
+
+215
+00:16:25,690 --> 00:16:29,590
+Hypersensitivity الhypersensitivity حلها سهل نعطيه
+
+216
+00:16:29,590 --> 00:16:32,510
+antihistamine و بنرابه و بنقلل ال syndrome سرعة و
+
+217
+00:16:32,510 --> 00:16:36,450
+انتهى الموضوع فيعني هي issue صحية لكن مش هال issue
+
+218
+00:16:36,450 --> 00:16:41,150
+بالمقارنة مع ال disease نفسهلكن لو انا بصحلي اصنع
+
+219
+00:16:41,150 --> 00:16:45,090
+او اخد ال immunoglobulin من ال human اكيد افضل ..
+
+220
+00:16:45,090 --> 00:16:50,490
+اكيد افضل .. لكن مش .. botulism صعب جدا لإن مافيش
+
+221
+00:16:50,490 --> 00:16:53,630
+حد بينصاب بال botulism و بصير عنده high bar
+
+222
+00:16:53,630 --> 00:16:56,510
+immunization بينما في ال .. في ال .. في ال .. في
+
+223
+00:16:56,510 --> 00:17:01,660
+تدولوز بصير هذا الكلامالمثال الثالث احنا قولنا
+
+224
+00:17:01,660 --> 00:17:08,220
+Botulism وقولنا تيتانوس وهي دفتيريا هما هما اللي
+
+225
+00:17:08,220 --> 00:17:12,360
+احنا قلنا عنهم بس ضدنا ال Botulism زيادة دفتيريا و
+
+226
+00:17:12,360 --> 00:17:16,780
+تيتانوس دفتيريا ال antitoxin is used in the
+
+227
+00:17:16,780 --> 00:17:18,800
+treatment of diphtheria طبعا الناس اللي تعرفين
+
+228
+00:17:18,800 --> 00:17:22,800
+اليوم دفتيريا تقريبا شبه شبه جرم موجودة يوم تالي
+
+229
+00:17:22,800 --> 00:17:26,500
+بالفعل ممكن ملاقيش حتى ال antitoxin في قطاع الأرض
+
+230
+00:17:26,500 --> 00:17:31,330
+كله ملاجونه لإن دفتيريا مش موجودةthe antitoxin can
+
+231
+00:17:31,330 --> 00:17:35,550
+neutralize مرة أخرى unbound كلمة مرة أخرى لأنه في
+
+232
+00:17:35,550 --> 00:17:38,590
+التنين اللي سبقوها نفس الجملة هتلاقوا it will
+
+233
+00:17:38,590 --> 00:17:43,170
+neutralize unbound diphtheria toxin أو heterotoxin
+
+234
+00:17:43,170 --> 00:17:46,450
+أو botulinum toxin قبل أن أنزل دليل على اللوحة
+
+235
+00:17:46,450 --> 00:17:51,750
+الصادقة ال botulism ال botulism فيه منه ست أنواع
+
+236
+00:17:51,750 --> 00:17:58,810
+toxins أشهر تلاتة هم A وB وEE معروف مصدره من
+
+237
+00:17:58,810 --> 00:18:01,290
+الأسماك يعني لو كنا تونة و صار معانا تسامح معاهم
+
+238
+00:18:01,290 --> 00:18:06,110
+بعدين احكي انه من وين؟ من type A او type E عفوا A
+
+239
+00:18:06,110 --> 00:18:11,030
+و B من ال canned food الأخرى في الأخرين طب انا
+
+240
+00:18:11,030 --> 00:18:16,890
+بستخدمها شخص ان المريض مصاب بنوع A ولا B ولا ايه؟
+
+241
+00:18:16,890 --> 00:18:20,350
+لأ انا بعطيه antitoxin للتلاتة بسموها trivalent
+
+242
+00:18:26,630 --> 00:18:31,770
+موضوعها في موضوع ال .. ال .. ال .. ال antitoxins
+
+243
+00:18:31,770 --> 00:18:33,990
+المستخدمة في الألعاب و ال protection ك
+
+244
+00:18:33,990 --> 00:18:37,650
+presentation هنا تقدمنا خلال خصة أصدقاء اي معلومات
+
+245
+00:18:37,650 --> 00:18:41,030
+جديدة عن هذا الموضوع اللي هو ال antitoxins
+
+246
+00:18:41,030 --> 00:18:45,590
+المستخدمة في الألعاب إذا نستخدم كمرة three ..
+
+247
+00:18:45,590 --> 00:18:49,990
+three او three valiant antitoxins هم A و B و A
+
+248
+00:18:53,190 --> 00:18:56,930
+نفس الجثة بيحتوي على الـ Diphtheria إنه مصموعة من
+
+249
+00:18:56,930 --> 00:19:00,550
+الهرز وبالتالي في إمكانية إنه يعملنا Hypotensia
+
+250
+00:19:00,550 --> 00:19:03,610
+نيجي
+
+251
+00:19:03,610 --> 00:19:07,290
+الآن للـ Live Attenuated Bacterial Vaccines نحنا
+
+252
+00:19:07,290 --> 00:19:09,950
+حكينا عن الـ Killed، أربع، ستهم ولا لسه حافظوينهم
+
+253
+00:19:09,950 --> 00:19:14,530
+كوكسيلة، برنايتا، راكتسي، راكتسي، فيبروفيرا
+
+254
+00:19:14,530 --> 00:19:18,490
+ويارسينيا، بيستس الـ Live Attenuated Bacterial
+
+255
+00:19:18,490 --> 00:19:23,240
+Vaccinesأنا مثالي عليها كتير محترف جدا اللي هو ال
+
+256
+00:19:23,240 --> 00:19:28,180
+micro bacterium Bovis المستخدمة للحماية من ال
+
+257
+00:19:28,180 --> 00:19:31,240
+micro bacterium tuberculosis ال Bovis مستخدم
+
+258
+00:19:31,240 --> 00:19:36,660
+للحماية من مين؟ من اللي هو ال tuberculosis اسم ال
+
+259
+00:19:36,660 --> 00:19:38,740
+vaccine كنتوا بيخدتهوا طلعوا على كتافكوا هنا
+
+260
+00:19:38,740 --> 00:19:45,420
+بتلاقوا نغبة صغيرة دي ال BCGال بي سي جي اختصار ل
+
+261
+00:19:45,420 --> 00:19:48,560
+باسيلاس كلمة green اللي هي ال strain اللي استخدم
+
+262
+00:19:48,560 --> 00:19:54,540
+اول مرة في ال inoculation طبعا واضح لما اقول بوبس
+
+263
+00:19:54,540 --> 00:19:58,320
+بيحمينا من ال tuberculosis يعني في cross reaction
+
+264
+00:19:58,320 --> 00:20:03,300
+زي حكاية ال protease و ال kids it is recommended
+
+265
+00:20:03,300 --> 00:20:06,780
+for children at high risk طب احنا فجأة اذا كنا
+
+266
+00:20:06,780 --> 00:20:09,520
+بناخده يعني ماعنى حاجة ان احنا هنا at high risk
+
+267
+00:20:09,520 --> 00:20:12,700
+بلطاني بتاخدوش لأ لطو معنى حاجة ان هم ايه
+
+268
+00:20:21,280 --> 00:20:27,340
+احنا تقريبا في السنة بي .. معدل عشر على خمساشر
+
+269
+00:20:27,340 --> 00:20:31,740
+حالة تقريبا بتتشخص سنويا في قطار غزة اللي عدد
+
+270
+00:20:31,740 --> 00:20:35,700
+سكانه تقريبا مليون و800 لو جينا .. عملنا رقام ل ..
+
+271
+00:20:35,700 --> 00:20:39,040
+لكل ميت ألف شخص باللاجة حالنا، احنا و بريطانيا جرب
+
+272
+00:20:39,040 --> 00:20:43,010
+من بعضاحنا بناخد فاكسين و هم مابياخدوش فاكسين هذا
+
+273
+00:20:43,010 --> 00:20:46,210
+مؤشر لو انا لو ماخداش الفاكسين نسبة ال infection
+
+274
+00:20:46,210 --> 00:20:50,770
+هتطلعي هتزيد لكن في نقطة كتير مهمة اقولكوا ليش هو
+
+275
+00:20:50,770 --> 00:20:56,250
+موجه الفاكسين و ليش احنا لسه بناخد فاكسين و بصير
+
+276
+00:20:56,250 --> 00:21:00,970
+فينا infection صح ولا لأ يعني فينا مشكلة برضه الآن
+
+277
+00:21:00,970 --> 00:21:05,230
+بتعرفوا حاجة اسمها intradermal و subcutaneous و
+
+278
+00:21:05,230 --> 00:21:08,090
+intramuscular و intravenous تسمعوا .. بتسمعوا
+
+279
+00:21:08,090 --> 00:21:12,320
+فيهم؟في ناس اه قررت ان انا بتلاب سنة رابعة او
+
+280
+00:21:12,320 --> 00:21:14,820
+تالتة وفي ناس ماتسمعوش فيهم لإنهم بتلاب سنة تانية
+
+281
+00:21:14,820 --> 00:21:19,240
+Intradermal يعني في الجلد بكلمة في الجلد يعني مش
+
+282
+00:21:19,240 --> 00:21:23,280
+تحت الجلد تحت الجلد معناه subcutaneous لقد ال
+
+283
+00:21:23,280 --> 00:21:28,700
+vaccine مصمم طالع عليه ال vaccine مصمم انه نحطه في
+
+284
+00:21:28,700 --> 00:21:33,320
+مكان ال bacteria هي attenuated و life ضعيفة و
+
+285
+00:21:33,320 --> 00:21:37,300
+تعبانة و منهكة و بعدين نحطها في مكان غير مناسب
+
+286
+00:21:37,300 --> 00:21:42,750
+لنموها اللي هو الجلدتحت الجلد إذا وصلت الدم ممكن
+
+287
+00:21:42,750 --> 00:21:47,810
+تروح على الانفنور وتعملنا tuberculosis فبنحقرها في
+
+288
+00:21:47,810 --> 00:21:51,630
+الجلد و أظن كلمة جلد و تحت الجلد لو جينا جزء إبرة
+
+289
+00:21:51,630 --> 00:21:56,070
+رفيعة مش مسابقة كبيرة بينهم و الإهمال اللي ممكن
+
+290
+00:21:56,070 --> 00:21:58,550
+نشوفه من قبل الممرضين و المرضات اللي بيعملوا
+
+291
+00:21:58,550 --> 00:22:02,410
+بطاقهم تمام ممكن يخلل ال intradermal subcutaneous
+
+292
+00:22:03,710 --> 00:22:06,810
+فجزء من الخمساشر حاجة اللي حكيت انا و انا جزء منهم
+
+293
+00:22:06,810 --> 00:22:11,470
+قد يصل الى النصف هو tuberculosis ناتج اصلا عن مين؟
+
+294
+00:22:11,470 --> 00:22:15,890
+عن ال vaccination عن سوق ال vaccination و بريطانيا
+
+295
+00:22:15,890 --> 00:22:18,890
+ماتوجفت ال vaccination لما وجهوا عيب الحياة
+
+296
+00:22:18,890 --> 00:22:22,210
+الناجمة حتى بريطانيا في المادة في موضوع ال vaccine
+
+297
+00:22:22,210 --> 00:22:28,370
+عن ال vaccine اعلى من تلك الناجمة عن ال natural
+
+298
+00:22:28,370 --> 00:22:34,490
+infection course فوجفوه ال vaccineلكن بيكونوا
+
+299
+00:22:34,490 --> 00:22:38,810
+تعرفوا شغل ال tuberculosis من الأمراض الخطيرة جدا
+
+300
+00:22:38,810 --> 00:22:46,030
+dreadful بيسموها يعني مزعجة البكتيريا بضيعة النمو
+
+301
+00:22:46,030 --> 00:22:54,690
+وبالتالي علاجها صعب جدا لأنه في قاعدة بتقول the
+
+302
+00:22:54,690 --> 00:22:58,110
+more active the organism the more effective the
+
+303
+00:22:58,110 --> 00:22:59,110
+antimicrobial
+
+304
+00:23:02,500 --> 00:23:07,280
+كلما زاد نشاط الميكروب كلما زاد نشاط العامل القاتل
+
+305
+00:23:07,280 --> 00:23:11,360
+للبكتيريا يعني البكتيريا النشيطة بتموت بسرعة بس
+
+306
+00:23:11,360 --> 00:23:16,200
+البكتيريا البطيئة بتموت ببطء شديد جدا لإن ال
+
+307
+00:23:16,200 --> 00:23:20,060
+metabolism تبعتها بتكون بطيئة وال antibiotic twist
+
+308
+00:23:20,060 --> 00:23:24,340
+على selected points أو نقاط معينة في ال metabolism
+
+309
+00:23:24,340 --> 00:23:29,670
+فال E.coli علاجها تلت أيام لخمس أيامالـ
+
+310
+00:23:29,670 --> 00:23:32,790
+Mycobacterium tuberculosis أقصر فترة علاج حصلنا
+
+311
+00:23:32,790 --> 00:23:35,950
+عليها ستة أشهر بأربع أنواع من المضادات الحيوية
+
+312
+00:23:35,950 --> 00:23:42,490
+بأربع أنواع من المضادات الحيوية بكتيريا مزعجة سيئة
+
+313
+00:23:42,490 --> 00:23:47,630
+قاتلة كثير من الناس كانوا يموتوا من ال .. و
+
+314
+00:23:47,630 --> 00:23:49,650
+تاريخيا أدراء و علماء
+
+315
+00:24:01,310 --> 00:24:06,310
+لأن كلهم بيكلوش، هذا بيكلش لإنه معوش و هذا بيكلش
+
+316
+00:24:06,310 --> 00:24:11,670
+لإنه مش فاضي، فبالتالي أقصد إن صورة التغذية بيخفل
+
+317
+00:24:11,670 --> 00:24:17,560
+المناعة و بيسهل الإصابة بـ tuberculosisعشانك احنا
+
+318
+00:24:17,560 --> 00:24:22,220
+اقعد risk لسه تمام؟ وبالتالي ال vaccination مازالة
+
+319
+00:24:22,220 --> 00:24:26,040
+موجودة لكن انا بقول لو في evaluation مستمر للبرامج
+
+320
+00:24:26,040 --> 00:24:29,260
+بعد ال vaccine انا بعتقد انه ممكن في يوم من الأيام
+
+321
+00:24:29,260 --> 00:24:33,160
+ناخد قرار ان احنا برضه تماما نوقف ال vaccine لان
+
+322
+00:24:33,160 --> 00:24:36,200
+تقريرا صبنا fifty fifty natural و vaccine acquired
+
+323
+00:24:36,200 --> 00:24:39,320
+فاكرين السنة الماضية في المرة الماضية؟ كانوا يعني
+
+324
+00:24:39,320 --> 00:24:43,120
+واحد من ال vaccine سبعونها تمام؟ هو capsular
+
+325
+00:24:43,120 --> 00:24:45,880
+والتاني هي اتوا killed
+
+326
+00:24:51,120 --> 00:24:55,480
+نفس الجملة، نفس الفقرة، يمكن أن نفس الكلمات
+
+327
+00:24:55,480 --> 00:24:58,940
+هتلاقوها مذكورة لسنواتها في الفاكسين الأولاني، it
+
+328
+00:24:58,940 --> 00:25:02,540
+is indicated for persons who are living or
+
+329
+00:25:02,540 --> 00:25:03,760
+traveling in areas
+
+330
+00:25:10,500 --> 00:25:13,940
+for persons who are in close contact with either
+
+331
+00:25:13,940 --> 00:25:17,840
+infected or caring نفس الجملة ذكرت المرضى الماضية
+
+332
+00:25:17,840 --> 00:25:22,760
+ان اما ده ناس بيعيشوا او مسافرين الى مناطق موبوعة
+
+333
+00:25:22,760 --> 00:25:27,800
+او لناس مصاحبين لو ناس مصابين او حاملين المرضى
+
+334
+00:25:27,800 --> 00:25:32,600
+مابناش تيفورد ماري تمشي في المجتمع مرة اخرى اي
+
+335
+00:25:32,600 --> 00:25:36,480
+واحد career بيتم اكتشاف ويجب ان يعالج حتى مع ان هو
+
+336
+00:25:36,480 --> 00:25:37,320
+مش مريض
+
+337
+00:25:43,150 --> 00:25:46,250
+microbe ماكنت سمعنا فيه كثير اسمه francicilla
+
+338
+00:25:46,250 --> 00:25:50,830
+tolerances francicilla tolerances هم الأذانب اعتقد
+
+339
+00:25:50,830 --> 00:25:55,130
+انه مرة معناها ذكرناها rabbits fever او tularemia
+
+340
+00:25:55,130 --> 00:26:00,430
+francicilla tolerances اقرأ جملة الأخيرة اللي في
+
+341
+00:26:00,430 --> 00:26:04,170
+الفقرة اللي بتقول francicilla tolerances is highly
+
+342
+00:26:04,170 --> 00:26:08,350
+infectious يعني contagious يعني لو احنا اتعاملنا
+
+343
+00:26:08,350 --> 00:26:12,810
+معاها في المختبركمايكروب بدنا نكون حاذرين جدا و
+
+344
+00:26:12,810 --> 00:26:17,330
+نلبس ال .. ال .. ال protective equipment سبعتنا
+
+345
+00:26:17,330 --> 00:26:21,910
+بشكل جيد و ناخد proper يعني safety precautions لنا
+
+346
+00:26:21,910 --> 00:26:26,070
+ولا غيرنا لإن البكتيريا جونا عنها ماضي ال vaccine
+
+347
+00:26:26,070 --> 00:26:30,010
+بيحتوي على attenuated .. live attenuated francilla
+
+348
+00:26:30,010 --> 00:26:35,030
+tolerances و يستخدم في أغراض محددة صيدين .. صيدين
+
+349
+00:26:35,030 --> 00:26:38,870
+حيوانة بيصيدون أسماكVeterinarians اللي هم الطباء
+
+350
+00:26:38,870 --> 00:26:44,550
+البيطاريين و Laboratory personals حضراتكم، لكن
+
+351
+00:26:44,550 --> 00:26:46,770
+طبعا احنا احنا اصلا ما بنا واحد اناش بكتيريا اصلا
+
+352
+00:26:46,770 --> 00:26:53,040
+مولي انا في اخضارولم يبحث عنها أحد من قبل على
+
+353
+00:26:53,040 --> 00:26:56,100
+الرغم من أن هنا فيها القرانة البرية و بعرفش بتاعه
+
+354
+00:26:56,100 --> 00:26:59,900
+القرانة البرية إلها مخالب نوعا ما يعني و عند
+
+355
+00:26:59,900 --> 00:27:05,420
+استيادها لأنها حرة مش مشتغلة في منازل ممكن خلال
+
+356
+00:27:05,420 --> 00:27:11,190
+عراقها للتخلص من الشخص اللي حملها ممكن تاخد شوقعند
+
+357
+00:27:11,190 --> 00:27:15,410
+الذبح و التنظيف للأرالب إذا كان الأبناء نفسه موصار
+
+358
+00:27:15,410 --> 00:27:18,830
+ممكن لذلك كتير من الناس اللي بيشتغلوا في تنظيف
+
+359
+00:27:18,830 --> 00:27:21,850
+الحيوانات تمام اللي منها الأرالب قد يصيب ببعض
+
+360
+00:27:21,850 --> 00:27:25,690
+التقرحات ناتجها عن اللي هو ال French cellulose و
+
+361
+00:27:25,690 --> 00:27:28,430
+أشكالها متنوعة و مختلفة قد تكون بصيرة و قد تكون
+
+362
+00:27:28,430 --> 00:27:31,850
+قاطلة تشبه الtyphoid fever يعني أنماء مختلفة من
+
+363
+00:27:31,850 --> 00:27:34,070
+العدوى هدرسها ان شاء الله في ال diagnostic micro
+
+364
+00:27:35,880 --> 00:27:39,760
+طيب احنا بتاعنا شغال كتير ال vaccines بدنا نحاول
+
+365
+00:27:39,760 --> 00:27:44,140
+اللخص بس خلينا .. ده طبعا كتير صغير هذا اللوحة
+
+366
+00:27:44,140 --> 00:27:48,120
+اللي هو .. هذا عبارة عن .. هعطيكم تلخيص ل .. لكل
+
+367
+00:27:48,120 --> 00:27:52,940
+اللي حاكناه، هذا عبارة عن قائمة بأسماء ال ..
+
+368
+00:27:52,940 --> 00:27:57,060
+البكتريات والتضاييمات اللي إلها أو البكتريات اللي
+
+369
+00:27:57,060 --> 00:28:01,430
+إلها تضاييمات في الولايات المتحدةكاتبين البكتيريا
+
+370
+00:28:01,430 --> 00:28:04,530
+والمرض والانتيجين المستخدم احنا بنتكلم عن vaccine
+
+371
+00:28:04,530 --> 00:28:08,270
+gathering فانتوا مش شايفين خليني اقرألكوا انا
+
+372
+00:28:08,270 --> 00:28:12,270
+قرأها انهم مش شايفين اللوحة حجم صغير
+
+373
+00:28:39,080 --> 00:28:46,530
+لحظو في التصنيف كلمة special situationاللي تحتها
+
+374
+00:28:46,530 --> 00:28:49,690
+اللي هو ال BCG احنا لدينا مش special situation هي
+
+375
+00:28:49,690 --> 00:28:52,710
+من وين؟ من ال common يعني ممكن احنا لو بدنا ندلوا
+
+376
+00:28:52,710 --> 00:28:58,390
+اللي احنا خاص بينا هنرفعها في القائمة اللي بقى ال
+
+377
+00:28:58,390 --> 00:29:02,190
+Yersinia pestis هي عبارة عن عبارة عن أيش برضه؟ مش
+
+378
+00:29:02,190 --> 00:29:12,170
+common use انها special situation طيب
+
+379
+00:29:12,170 --> 00:29:21,110
+ناخد يعني خدنا نقولحكمة او استخلاص او خلاصة من
+
+380
+00:29:21,110 --> 00:29:26,090
+الموضوع تبعنا هذا فيما يتعلق فيما يتعلق بال
+
+381
+00:29:26,090 --> 00:29:31,030
+vaccines او البكتيريا ال vaccine بشكل عام و ارجو
+
+382
+00:29:31,030 --> 00:29:34,270
+انكوا تركزوا معايا عشان بدنا نحاول بالفعل يعني
+
+383
+00:29:34,270 --> 00:29:37,330
+انلخص
+
+384
+00:29:37,330 --> 00:29:42,580
+المعلومات بطريقة سهلة جداهنا قلنا ال bacterial
+
+385
+00:29:42,580 --> 00:29:46,280
+vaccines او ال vaccination بشكل عام تتلخص بشغلتين
+
+386
+00:29:46,280 --> 00:29:50,540
+active و passive، شفنا ال passive و عرفناه بحاجة
+
+387
+00:29:50,540 --> 00:29:55,740
+واحدة antibodies و ال antibody اللي قرصناهم لحد
+
+388
+00:29:55,740 --> 00:30:01,970
+الان هم كلهم antitoxinsانتي توكسين، انتي تيتروس،
+
+389
+00:30:01,970 --> 00:30:07,630
+انتي غوتشيليتن و انتي ديثيريا كلهم انتي توكسين، to
+
+390
+00:30:07,630 --> 00:30:12,170
+neutralize كان غلط واحد، to neutralize unbound،
+
+391
+00:30:12,170 --> 00:30:17,970
+unbound يعني الغير مرتبط، unbound toxinsأخدنا
+
+392
+00:30:17,970 --> 00:30:22,330
+نموذج دمش بين الحالتين بين ال active immunity و
+
+393
+00:30:22,330 --> 00:30:25,570
+بين ال passive immunity النموذج الماضي اللي
+
+394
+00:30:25,570 --> 00:30:29,010
+ذكرناها كان الفيروسات اللي هو ال rabies ده الكلب
+
+395
+00:30:29,010 --> 00:30:33,170
+السعادة بس احنا ماندلسش يا دي فيروس بكسين ماندلسش
+
+396
+00:30:33,170 --> 00:30:36,750
+يا دي مين انا؟ ماندلس بكتيريا لبكسين فالمثال
+
+397
+00:30:36,750 --> 00:30:42,850
+الرائع على ال activeهو ان انسان يوصاب يوصاب
+
+398
+00:30:42,850 --> 00:30:47,910
+بتتانوس، بنعطيه antitoxin و بنعطيه في نفس الوجه
+
+399
+00:30:47,910 --> 00:30:51,690
+التوكسويد، بس في مكانين مختلفة، يعني إذا اعطيناه
+
+400
+00:30:51,690 --> 00:30:54,330
+يعمل ايه، ده دي، بنعطيه توكسويد، ده نعطيه
+
+401
+00:30:54,330 --> 00:30:59,530
+antitoxin، ليش؟لو انه ارتقط توكسين بالانتيتوكسين،
+
+402
+00:30:59,530 --> 00:31:04,330
+ايش هيمله؟ يرجع لا توكسويد هيكون فائد وفائدة ولا
+
+403
+00:31:04,330 --> 00:31:08,730
+الانتيتوكسين هيكون له فائد، تمام؟ فهي نموذج على ..
+
+404
+00:31:08,730 --> 00:31:12,210
+اخدرصنا هي نموذج واحد في البكتيريا استخدمنا ال
+
+405
+00:31:12,210 --> 00:31:17,040
+active وال passive immunizationنخلع للـ Vaccine
+
+406
+00:31:17,040 --> 00:31:22,200
+للـ Active Immune أخدنا تلات مواضيع رئيسية قلنا
+
+407
+00:31:22,200 --> 00:31:26,760
+عنهم اللي هم ال capsular polysaccharide و ال
+
+408
+00:31:26,760 --> 00:31:30,560
+capsular polysaccharide كان في تلاتة فاكرينهم؟ أول
+
+409
+00:31:30,560 --> 00:31:34,660
+واحدة Steptococcus pneumonia سبعة و عشرين و تلاتة
+
+410
+00:31:34,660 --> 00:31:38,980
+و تلاتة عشرين و سبعة مجموعهم تلاتين تلاتة عشرين و
+
+411
+00:31:38,980 --> 00:31:45,000
+سبعةو عرفنا متى بنستخدم التاني عن الأول و الأول
+
+412
+00:31:45,000 --> 00:31:47,180
+متى بنستخدمه في أي ظروف
+
+413
+00:31:58,470 --> 00:32:03,210
+واخدنا Hemophilus influenza وكان فيها one zero
+
+414
+00:32:03,210 --> 00:32:06,810
+type اللي هو Hemophilus influenza اللي هو قدامكوا
+
+415
+00:32:06,810 --> 00:32:12,430
+على اللوحة الآن رقم اتنين في ال bacterial vaccines
+
+416
+00:32:12,430 --> 00:32:16,950
+Hemophilus influenza type B ويُقتصر ب HIB HIB
+
+417
+00:32:16,950 --> 00:32:23,030
+vaccine يعني Hemophilus influenza type B vaccine
+
+418
+00:32:23,030 --> 00:32:30,750
+بعدين انتقلنا منالكابسول للتوكسايد وفي التوكسايد
+
+419
+00:32:30,750 --> 00:32:35,110
+ال DBT كان هو المسيطر واتفقنا على انه ال deuterium
+
+420
+00:32:35,110 --> 00:32:39,870
+مافي مشكلة تقريبا completely prevented التيتانوس
+
+421
+00:32:39,870 --> 00:32:44,370
+تقريبا completely prevented بال .. بالتوكسايد
+
+422
+00:32:44,370 --> 00:32:48,790
+vaccine ده لنا مشكلة تسميه اننا ال pertosis و ال
+
+423
+00:32:48,790 --> 00:32:53,790
+pertosisأنا بقولكوا لحد الآن في حالات في العالم
+
+424
+00:32:53,790 --> 00:32:57,270
+برتوزز يعني حتى مع الفاكسين الجديد مع الفاكسين
+
+425
+00:32:57,270 --> 00:33:01,130
+الجديد يعني يبدو ان البعض مثل اللي احنا يعني المرة
+
+426
+00:33:01,130 --> 00:33:03,870
+الماضية يقولنا لما حصلنا نفهم اكتر عنه يبدو انهم
+
+427
+00:33:03,870 --> 00:33:07,540
+مش فاهمين كل الصورة لسهلأن حتى في وجود ال
+
+428
+00:33:07,540 --> 00:33:11,960
+Acellular vaccine كان فرح في email إيجاري من
+
+429
+00:33:11,960 --> 00:33:14,660
+البروميد اللي قلتلكم .. اللي بتتمانية بال
+
+430
+00:33:14,660 --> 00:33:19,960
+infectious disease، new cases of pertosis in USA،
+
+431
+00:33:19,960 --> 00:33:23,260
+و USA اللي هو فترة بستخدمهمين اللي هو ال .. ال ..
+
+432
+00:33:23,260 --> 00:33:30,370
+ال Acellular pertosis vaccineفصراحة مشكلة لإن هنا
+
+433
+00:33:30,370 --> 00:33:37,210
+تكسويد لحاله و لا نعطيه مركبة تاعة لمركبات تانية و
+
+434
+00:33:37,210 --> 00:33:42,670
+تباهينا قبل قليل بإنه تكسويد بتاع البرتروسيز هو
+
+435
+00:33:42,670 --> 00:33:45,530
+the first genetically modified أو genetically
+
+436
+00:33:45,530 --> 00:33:49,010
+engineered تكسويد ما أنتجناه بال chemical
+
+437
+00:33:49,010 --> 00:33:52,790
+modification و إنما بالهدسة الوراثية و أصبحنا من
+
+438
+00:33:52,790 --> 00:33:55,950
+خلال فعطيل two amino acids ننتج
+
+439
+00:34:00,890 --> 00:34:05,730
+وبعد انتقالنا اللى هو ال killed وارفنا مجموعة منهم
+
+440
+00:34:07,320 --> 00:34:11,020
+الاربع اللي حفظناها ماحفظوها مش لسه احنا تعملنا
+
+441
+00:34:11,020 --> 00:34:15,120
+انه نعيد و نزيد فيان المحاضرة في ال vaccines لإنه
+
+442
+00:34:15,120 --> 00:34:18,500
+قد تكون confusing لكن نطلب منكوا التالي انه كل
+
+443
+00:34:18,500 --> 00:34:22,080
+واحد يعمل his own diagram عارفينش دياجرام؟ يعني
+
+444
+00:34:22,080 --> 00:34:26,340
+يفصل من البداية انا بكتب ال vaccination اكتف باسف
+
+445
+00:34:26,340 --> 00:34:29,440
+فالباسف ايش اللي درسوا في ال .. و كل واحد او mind
+
+446
+00:34:29,440 --> 00:34:32,580
+map يعملهم و اللي بيعمل ال mind map محترم اللي
+
+447
+00:34:32,580 --> 00:34:36,620
+هتشوفته هنسامهم ال representation يعمل mind map
+
+448
+00:34:38,370 --> 00:34:42,390
+واحدة لل chapter هذا هنسرها بال presentation خلاص
+
+449
+00:34:42,390 --> 00:34:46,890
+الموضوع صار لنا صار لنا معلومات بتخص ال
+
+450
+00:34:46,890 --> 00:34:50,510
+vaccination لكن هذه المعلومات كما هو علم المناعة
+
+451
+00:34:50,510 --> 00:34:54,750
+متغيرة كل فترة و فترة بنكشف معلومات جديدة وبتالي
+
+452
+00:34:54,750 --> 00:34:58,810
+بنلاقي أنهفي نقطتين مهمين اللي هتاخدها البقية اللي
+
+453
+00:34:58,810 --> 00:35:02,390
+هتبقى ال geographical distribution اللي علاقة بالـ
+
+454
+00:35:02,390 --> 00:35:04,850
+Epidemiology صح ولا لا الأمار اللي موجودة في
+
+455
+00:35:04,850 --> 00:35:07,890
+فلسطين غير الأمور اللي موجودة مثلا في السودان احنا
+
+456
+00:35:07,890 --> 00:35:10,370
+عندهم ملاريا احنا ماعندك ملاريا فهل ما هال من
+
+457
+00:35:10,370 --> 00:35:13,310
+المنطقى انه لما نسافر على فلسطين تشبرني لو فيه لا
+
+458
+00:35:13,310 --> 00:35:16,390
+فيه vaccine للملاريا انه ناخد vaccine لأ طبعا
+
+459
+00:35:16,390 --> 00:35:20,290
+فبالتالي كل دولة اللي هخلصيتها في موضوع ال vaccine
+
+460
+00:35:20,290 --> 00:35:25,360
+هي رقم واحد رقم اتنين معلوماتنا بتتغيرفبالتالي ما
+
+461
+00:35:25,360 --> 00:35:31,820
+نقولش انه فيه جدول جدول معد لكل العالم من ال
+
+462
+00:35:31,820 --> 00:35:35,380
+vaccines قد نشترك في بعض الأمرض الميسلز العالمية
+
+463
+00:35:35,380 --> 00:35:38,820
+في كل الدنيا موجودة لكن قد كلنا خصيات في بعض اللي
+
+464
+00:35:38,820 --> 00:35:41,860
+هو الأمراض مثل كوكسيلابرناتوش موجودة عندنا على
+
+465
+00:35:41,860 --> 00:35:45,520
+سبيل المثال فبالتالي ما نجبراش ناخد على ال vaccine
+
+466
+00:35:45,520 --> 00:35:50,540
+إذا هناك إمكانية أنه فيه اختلاف من دولة إلى دولة
+
+467
+00:35:56,990 --> 00:36:00,710
+Vaccines recommended for children aged 6 to 9 سنة
+
+468
+00:36:00,710 --> 00:36:05,510
+2014 انا متأكد ان هناك نوع من ايه من الاختلاف
+
+469
+00:36:05,510 --> 00:36:07,810
+فبخلي نقرأهم بالمرة و نختم فيهم المحاضرة اللي
+
+470
+00:36:07,810 --> 00:36:11,550
+بعتنا البكتيريات توكسيين فيها دفتيرياتوكسايت
+
+471
+00:36:11,550 --> 00:36:13,510
+تيتانوستوكسايت ايسيريوابيرتوز
+
+472
+00:36:15,570 --> 00:36:19,010
+المفلس انفلوانزا type B وهو الـ HIT و الـ
+
+473
+00:36:19,010 --> 00:36:20,970
+meningococcal الواكسين اللي هم الأربع اللي حكينا
+
+474
+00:36:20,970 --> 00:36:27,850
+عنهم الـ A و الـ C و الـ W 135 و الـ 153 و الـ 135
+
+475
+00:36:27,850 --> 00:36:29,610
+.. 135 .. 135 .. 135 .. 135 .. 135 .. 135 .. 135
+
+476
+00:36:29,610 --> 00:36:30,430
+.. 135 .. 135 .. 135 .. 135 .. 135 .. 135 .. 135
+
+477
+00:36:30,430 --> 00:36:31,130
+.. 135 .. 135 .. 135 .. 135 .. 135 .. 135 .. 135
+
+478
+00:36:31,130 --> 00:36:31,610
+.. 135 .. 135 .. 135 .. 135 .. 135 .. 135 .. 135
+
+479
+00:36:31,610 --> 00:36:32,690
+.. 135 .. 135 .. 135 .. 135 .. 135 .. 135 .. 135
+
+480
+00:36:32,690 --> 00:36:35,110
+.. 135 .. 135 .. 135 .. 135 .. 135 .. 135 .. 135
+
+481
+00:36:35,110 --> 00:36:39,770
+..
+
+482
+00:36:45,510 --> 00:36:49,450
+هي Hepatitis A وفي غزة مش هي Hepatitis A ك vaccine
+
+483
+00:36:49,450 --> 00:36:53,450
+ك vaccine مش موجود للأسف ولا ال rotavirus موجود
+
+484
+00:36:53,450 --> 00:36:57,730
+ولا ال varicella كمان فإيش لإن احنا عندنا
+
+485
+00:36:57,730 --> 00:37:02,230
+Hepatitis B عندنا ال MMR اللي هو ال missiles و ال
+
+486
+00:37:02,230 --> 00:37:05,530
+mumps و ال rubella عندنا ال polio تمام فبال
+
+487
+00:37:05,530 --> 00:37:12,090
+rotavirus عالم المعرفة عالم المعرفة تودي إلى أنه
+
+488
+00:37:12,090 --> 00:37:16,810
+ما نهتمش من أن أنا مش عارففالروتا فايروس انعمل
+
+489
+00:37:16,810 --> 00:37:19,790
+ديالو أسافراز عن انطلاق المال استيقظ طبعا أبحاث
+
+490
+00:37:19,790 --> 00:37:24,270
+لأنه أكتر من 15% أو 17% لقد كان بالضبط الرقم لكن
+
+491
+00:37:24,270 --> 00:37:29,290
+في الحدود هذه من الديارياء caused in children by
+
+492
+00:37:29,290 --> 00:37:33,310
+rotavirus فبعد أنه منعملش كروتين و لأن الوزارة
+
+493
+00:37:33,310 --> 00:37:36,670
+بتعملوش يعني reporting و بتاعي ماحد بيعرف أن في
+
+494
+00:37:36,670 --> 00:37:39,850
+عندها rotavirus و بتعرفش ليه ليش ال vaccine ليش ال
+
+495
+00:37:39,850 --> 00:37:44,450
+vaccineبينما ال .. ال .. ال .. ال .. ال .. ال ..
+
+496
+00:37:44,450 --> 00:37:44,470
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+497
+00:37:44,470 --> 00:37:44,690
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+498
+00:37:44,690 --> 00:37:45,030
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+499
+00:37:45,030 --> 00:37:46,470
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+500
+00:37:46,470 --> 00:37:47,390
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+501
+00:37:47,390 --> 00:37:48,430
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+502
+00:37:48,430 --> 00:37:53,410
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+503
+00:37:53,410 --> 00:37:54,370
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+504
+00:37:54,370 --> 00:37:54,690
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+505
+00:37:54,690 --> 00:37:56,910
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+506
+00:37:56,910 --> 00:38:01,240
+ال .. ال .. اليكون فيه دراسة و أرقام هتعطينا
+
+507
+00:38:01,240 --> 00:38:05,320
+إمكانية انه نقرر ناخد vaccine نطعم الناس لو ما
+
+508
+00:38:05,320 --> 00:38:09,660
+نطعمش الناس ضد هذا المرض انا بأنا نوجب نظري انه ال
+
+509
+00:38:09,660 --> 00:38:13,060
+rotavirus تطيعنا أولا من تطيعنا ال Structococcus
+
+510
+00:38:13,060 --> 00:38:16,740
+pneumonia في قطاعنا لإن ال balance بتاعها أعلى من
+
+511
+00:38:16,740 --> 00:38:20,640
+الكثير و دايما تذكروا وإن كانت self-limited ال
+
+512
+00:38:20,640 --> 00:38:25,880
+rotavirus الا انه ممكن تعمل أي دبارية ممكن تعمل
+
+513
+00:38:27,900 --> 00:38:33,940
+الانيميا لان الطفل هو في فترة الانتصار سبعه مش
+
+514
+00:38:33,940 --> 00:38:35,960
+مجتمع و هو بالتالي ممكن يصير عنده انيميا، الانيميا
+
+515
+00:38:35,960 --> 00:38:38,880
+ممكن تأثرة حتى على الذكاء و التحصيل العقلي عند
+
+516
+00:38:38,880 --> 00:38:44,320
+الطفل فالدولة يجب ان تولى اهتمام اكبر في موضوع
+
+517
+00:38:44,320 --> 00:38:47,420
+اللي هو ال vaccination اعتقد ان اخيرا المرة اللي
+
+518
+00:38:47,420 --> 00:38:50,940
+جاي ان شهرها هيكون موضوعنا عن ال antimicrobials ال
+
+519
+00:38:50,940 --> 00:38:52,120
+antibiotics
+
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/IMdvAWrllOc.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/IMdvAWrllOc.srt
new file mode 100644
index 0000000000000000000000000000000000000000..728343e47be8c4301b703a6f1c73d63a3699a4c5
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/IMdvAWrllOc.srt
@@ -0,0 +1,2443 @@
+1
+00:00:20,670 --> 00:00:25,430
+بسم الله الرحمن الرحيم احنا انتهينا آخر مرة 
+
+2
+00:00:25,430 --> 00:00:28,490
+لما تكلمنا عن قدرة الميكروبات على 
+
+3
+00:00:28,490 --> 00:00:32,810
+intracellular existence وقسمنا البكتيريا لصنفين
+
+4
+00:00:32,810 --> 00:00:35,470
+و قلنا اللي هم الصنف اللي obligate intracellular
+
+5
+00:00:35,470 --> 00:00:39,950
+و الثاني اللي هو ال facultative ده شفنا مجموعة من 
+
+6
+00:00:39,950 --> 00:00:44,670
+tactics خلينا نسميها أو ال ... ال ... ال ... ال ... ال
+
+7
+00:00:44,670 --> 00:00:46,150
+... ال ... ال ... ال ... ال ... ال ... ال ... ال ... ال ...
+
+8
+00:00:46,150 --> 00:00:46,550
+ال ... ال ... ال ... ال ... ال ... ال ... ال ... ال ... ال
+
+9
+00:00:46,550 --> 00:00:46,770
+... ال ... ال ... ال ... ال ... ال ... ال ... ال ... ال ...
+
+10
+00:00:46,770 --> 00:00:46,870
+ال ... ال ... ال ... ال ... ال ... ال ... ال ... ال ... ال
+
+11
+00:00:46,870 --> 00:00:47,150
+... ال ... ال ... ال ... ال ... ال ... ال ... ال ... ال ...
+
+12
+00:00:47,150 --> 00:00:48,270
+ال ... ال ... ال ... ال ... ال ... ال ... ال ... ال ... ال
+
+13
+00:00:48,270 --> 00:00:53,340
+... التي من خلالها البكتيريا استطاعت أن تقاوم
+
+14
+00:00:53,340 --> 00:00:58,860
+الـphagocytic killing، إما عن طريق الارتباط بالـlysosome أو
+
+15
+00:00:58,860 --> 00:01:04,340
+كانت نفسها resistant للـlysosome الـdestruction، يعني 
+
+16
+00:01:04,340 --> 00:01:08,180
+في مجموعة كبيرة من الـtactics، اللي هو في الكلمة
+
+17
+00:01:08,180 --> 00:01:10,780
+معاها موضوع مختلف شوية، اللي هو الـantigenic
+
+18
+00:01:10,780 --> 00:01:17,050
+heterogenicity كنا متفقين كنا متفقين على أن جهاز
+
+19
+00:01:17,050 --> 00:01:21,950
+المناعة لديه القدرة على التعرف على بعض المكونات
+
+20
+00:01:21,950 --> 00:01:26,530
+البكتيرية أو الفيروسية أو الطفيلية كأنتجينات ومن
+
+21
+00:01:26,530 --> 00:01:31,190
+ثم تكوين أجسام مضادة ضد هذه الأنتجينات وبالتالي في
+
+22
+00:01:31,190 --> 00:01:35,530
+المرة القادمة بيصير فيه من السهل جدا على جهاز
+
+23
+00:01:35,530 --> 00:01:40,290
+المناعة أن يكافح هذه الميكروبات لكن بالنسبة 
+
+24
+00:01:40,290 --> 00:01:43,310
+للبكتيريا ... لكن بالنسبة للبكتيريا أيضا لديها 
+
+25
+00:01:43,310 --> 00:01:47,310
+tactics هي كمان برضه عندها القدرة على تغيير الـ
+
+26
+00:01:47,310 --> 00:01:52,010
+Antigenic Variation وبالتالي كل مرة بتدخل برقم
+
+27
+00:01:52,010 --> 00:01:58,430
+جديد أو بـantigen جديد، إذن هذه القدرة طبعا تم
+
+28
+00:01:58,430 --> 00:02:01,230
+إثباتها قدرة البكتيريا على تغيير الـantigenic تم
+
+29
+00:02:01,230 --> 00:02:05,030
+إثباتها in vitro وبتوقع تكون برضه موجودة في وين؟
+
+30
+00:02:05,030 --> 00:02:11,130
+في الـin vivo كل هذا to evade the immune system ولا
+
+31
+00:02:11,130 --> 00:02:16,170
+أريد أن أذكركم بمثال درسناه بشكل واقعي ال ... ال
+
+32
+00:02:16,170 --> 00:02:20,190
+... ال ... الـpili attachment الـpili تحت 
+
+33
+00:02:20,190 --> 00:02:26,630
+الـNeisseria gonorrhoeae وقلنا لكم أنها الـmain virulence
+
+34
+00:02:26,630 --> 00:02:30,310
+factor لأن أول خطوة في الـinfection كانت الـ
+
+35
+00:02:30,310 --> 00:02:34,710
+attachment وهي في موقع أصلا في مكان في سيول جارفة
+
+36
+00:02:34,710 --> 00:02:37,810
+وبالتالي حتى تستطيع أن تعمل infection لازم تبقى
+
+37
+00:02:37,810 --> 00:02:41,370
+هناك فجسم الإنسان تنبه لهذا الموضوع كأول أجسام
+
+38
+00:02:41,370 --> 00:02:46,350
+مضادة للـpili لكن البكتيريا تنبهت وواصلت برضه تعمل 
+
+39
+00:02:46,350 --> 00:02:52,090
+antigenic variation للـpili في نقطة أول اللوحة المهمة
+
+40
+00:02:52,090 --> 00:02:58,510
+التي بدنا نركز عليها كثيرا من الـviral instructors
+
+41
+00:03:00,830 --> 00:03:03,590
+كثيرا من الـvirulence factors بتكون هي نفسها الـ
+
+42
+00:03:03,590 --> 00:03:07,130
+antigen لكن في نفس الوقت ليس بالضرورة أن الـ
+
+43
+00:03:07,130 --> 00:03:12,170
+antigen يكون هو الـvirulence factor كم مرة أن كثيرا
+
+44
+00:03:12,170 --> 00:03:15,770
+من الـvirulence factors بحد ذاتها هي الـantigen
+
+45
+00:03:15,770 --> 00:03:20,710
+لكن ليس كل virulence factor هو الـantigen أو ليس
+
+46
+00:03:20,710 --> 00:03:25,620
+كل antigen هو virulence factor بنفس المعنى النقطة
+
+47
+00:03:25,620 --> 00:03:28,260
+الأخيرة في الـpart of Genesis التي هتكلم عنها اليوم
+
+48
+00:03:28,260 --> 00:03:31,780
+التي هي الـrequirement of iron برضه بتدرجوا فيكم
+
+49
+00:03:31,780 --> 00:03:35,140
+للسابق وأذكركم بأنه لما درسنا الـstructure of
+
+50
+00:03:35,140 --> 00:03:39,720
+bacteria ما درسناها لنحفظ مكونات و بس لما
+
+51
+00:03:39,720 --> 00:03:43,560
+اتكلمنا عن الـpigment قلنا أحد وظائف الـpigment
+
+52
+00:03:43,560 --> 00:03:48,620
+is iron binding فتتعرفوا تطلعوا على iron prediction
+
+53
+00:03:48,620 --> 00:03:52,480
+هانمّنّه في اللوحة التي أمامنا هلاقي أنه for the 
+
+54
+00:03:52,480 --> 00:03:56,540
+host the iron metabolism denies pathogenic
+
+55
+00:03:56,540 --> 00:04:00,600
+bacteria an adequate source ايش يعني؟ denies يعني
+
+56
+00:04:00,600 --> 00:04:07,280
+بيحرم البكتيريا من مصدر كافي للـiron بالنسبة
+
+57
+00:04:07,280 --> 00:04:13,410
+للبكتيريا they have developed several systems أو
+
+58
+00:04:13,410 --> 00:04:16,970
+mechanism by which they can obtain iron from the 
+
+59
+00:04:16,970 --> 00:04:21,010
+host واحد منها بيسموه الاسم high affinity iron
+
+60
+00:04:21,010 --> 00:04:24,590
+assimilation system high affinity iron
+
+61
+00:04:24,590 --> 00:04:28,530
+assimilation system أنا ما بدي أخد في تفاصيل
+
+62
+00:04:28,530 --> 00:04:33,970
+biochemistry كثيرة لكن من اللوحة القادمة مركب واحد
+
+63
+00:04:33,970 --> 00:04:39,120
+اسمه siderophores السايدروفورز هي iron binding
+
+64
+00:04:39,120 --> 00:04:43,280
+compounds مولودة في البكتيريا، لما تطلقها
+
+65
+00:04:43,280 --> 00:04:45,760
+البكتيريا زي ما حكيته بالضبط الـpigment اللي حكينا
+
+66
+00:04:45,760 --> 00:04:48,860
+عنها، لما تطلقها البكتيريا وتصير موجودة مع الـ
+
+67
+00:04:48,860 --> 00:04:53,720
+host، فترتبط بمين؟ بالـiron وبالتالي هذا الـiron is 
+
+68
+00:04:53,720 --> 00:04:57,400
+unavailable to the host لكن available لمن؟
+
+69
+00:04:57,400 --> 00:05:01,360
+للبكتيريا يبقى بنقدر نقول أن قدرة البكتيريا على
+
+70
+00:05:01,360 --> 00:05:06,220
+الارتباط أو الـuptake للـiron هي تعتبر virulence
+
+71
+00:05:06,220 --> 00:05:11,360
+factor لأن بتحرم مين؟ بتحرم الـhost طب الـhost ...
+
+72
+00:05:11,360 --> 00:05:14,140
+الـiron إله ... إله أهمية للـhost ... أكيد إله أهمية
+
+73
+00:05:14,140 --> 00:05:19,840
+للـhost وأنا بتأكد ... أنا بتأكد أن حرمان الـhost
+
+74
+00:05:19,840 --> 00:05:23,100
+من الـiron هيضعف جهاز المناعة، يعني بيبقى
+
+75
+00:05:23,100 --> 00:05:28,890
+advantage لمين؟ للبكتيريا اللي هو ... بأكيد بعد اللوحة
+
+76
+00:05:28,890 --> 00:05:32,510
+التي هي السابقة احنا بنكون أن أنهينا جزء كبير صحيح
+
+77
+00:05:32,510 --> 00:05:38,970
+من بعض الـdiseases في البكتيريا لكن ما زال لدينا
+
+78
+00:05:38,970 --> 00:05:44,290
+نقطة مهمة جدا وهي الـimmune system لأن لما
+
+79
+00:05:44,290 --> 00:05:48,130
+تكلمت عن الـinteraction في الـdisease قلنا فيهِن
+
+80
+00:05:48,130 --> 00:05:54,770
+ثلاثة عناصر قلنا العامل نفسه وهو معبر عن بعض
+
+81
+00:05:58,690 --> 00:06:02,330
+الـimmune system، ثلاثة أشياء مرتبطة وقلنا ضفنا
+
+82
+00:06:02,330 --> 00:06:04,830
+عليها بيضر عبرها، اللي هو الـportal of entry
+
+83
+00:06:04,830 --> 00:06:11,050
+اليوم بتبدأ نناقش بشكل سطحي تماما، يعني معناه آخر
+
+84
+00:06:11,050 --> 00:06:13,730
+لن ندخل في التفاصيل، لأن في مساق كامل اسمه
+
+85
+00:06:13,730 --> 00:06:16,950
+immunology، تدرسوه إن شاء الله في السنة القادمة
+
+86
+00:06:16,950 --> 00:06:21,110
+Immunity of the host، خليني أطلع على اللوحة باللون
+
+87
+00:06:21,110 --> 00:06:27,940
+العربي هناك نوعان من الخلايا المناعية التي تخرج من الـ
+
+88
+00:06:27,940 --> 00:06:33,320
+bone marrow وبنقدر نصنفهم على أنهم two lineages،
+
+89
+00:06:33,320 --> 00:06:37,620
+lineage يعني خطين، الخط الأول myeloid والخط الثاني
+
+90
+00:06:37,620 --> 00:06:40,910
+الذي هو الـlymphoid من الـmyeloid بيطلع عندنا الـ
+
+91
+00:06:40,910 --> 00:06:42,930
+erythrocytes والـplatelets والصحيحة دول اللي
+
+92
+00:06:42,930 --> 00:06:47,350
+بنتعلمهم مش احنا من جهاز المناعة لكن بيطلع منهم الـ
+
+93
+00:06:47,350 --> 00:06:51,750
+neutrophil والـmacrophages، إذن الـneutrophil والـ
+
+94
+00:06:51,750 --> 00:06:56,370
+macrophages هم من الـmyeloid tissue بينما الـ
+
+95
+00:06:56,370 --> 00:06:59,550
+lymphoid tissue بيطلع عندنا حاجة اسمها natural
+
+96
+00:06:59,550 --> 00:07:03,510
+killer، natural killer ومن اسمها يعني وظيفتها 
+
+97
+00:07:03,510 --> 00:07:09,720
+الأبدية to kill cells وبيطلع من عندنا منها نوعين
+
+98
+00:07:09,720 --> 00:07:15,820
+كمان مهمين جدا اسمهم T-cells وB-cells الـT-cells
+
+99
+00:07:15,820 --> 00:07:19,080
+طبعا برضه احنا مصنفينها هنا صنفين فقط اللي هم الـ
+
+100
+00:07:19,080 --> 00:07:25,760
+CD8 وCD4 والـCD8 وCD4 هم عبارة عن markers ايش
+
+101
+00:07:25,760 --> 00:07:29,580
+يعني markers يعني molecules نعم من خلال المتعارف على
+
+102
+00:07:29,580 --> 00:07:34,060
+الخلايا فاسمها حتى الاختصار اللي هو clusters of
+
+103
+00:07:34,060 --> 00:07:36,980
+differentiation CD معناها cluster of
+
+104
+00:07:36,980 --> 00:07:42,720
+differentiation ففيها مجموعة CD8 ومجموعة CD4 الآن
+
+105
+00:07:42,720 --> 00:07:49,760
+الـCD8 بينبتق عنها خلايا اسمها cytotoxic لحظة
+
+106
+00:07:49,760 --> 00:07:54,420
+cytotoxic T cells ايش وظيفتها هذه الـcytotoxic T
+
+107
+00:07:54,420 --> 00:07:59,150
+cells برضه قتل يبقى فيه natural killer من الـ
+
+108
+00:07:59,150 --> 00:08:04,010
+lymphoid والـcytotoxic T cell من اللي هو ال ...
+
+109
+00:08:04,010 --> 00:08:08,450
+برضه من الـlymphoid من ال ... من الـlymphoid الـCD4
+
+110
+00:08:08,450 --> 00:08:13,350
+بيطلع منها بناء TH1 وTH2 وTH1 و
+
+111
+00:08:13,350 --> 00:08:19,050
+TH2 بيعتبروا essential أو الـmastermind للـ
+
+112
+00:08:19,050 --> 00:08:22,050
+humoral immune response يعني هم اللي بيعملوا
+
+113
+00:08:22,050 --> 00:08:26,670
+orchestrated action في الـimmune system لذلك لما
+
+114
+00:08:26,670 --> 00:08:30,850
+بتنصاب الخلايا هذه من قبل فيروس الـaids، ايش بصير
+
+115
+00:08:30,850 --> 00:08:40,010
+للمريض؟ بيفقد المناعة بحسب عدد الخلايا المصابة
+
+116
+00:08:40,010 --> 00:08:41,190
+فالـCD4
+
+117
+00:08:48,390 --> 00:09:04,630
+الـB-cells هي المسؤولة
+
+118
+00:09:04,630 --> 00:09:09,770
+عن إنتاج الـantibodies طبعا هنشوف كمان لوحة توضح
+
+119
+00:09:09,770 --> 00:09:15,030
+هذا الكلام بشكل أكثر لكن الـB-cells إذا اتّصل بها
+
+120
+00:09:15,030 --> 00:09:22,660
+antigen specific إلها وكأنه إشارة يلا بيسل انقسمي،
+
+121
+00:09:22,660 --> 00:09:27,760
+ابتدأ تنقسم انقسامات، فاكرين كلمة سلالة؟ clone،
+
+122
+00:09:27,760 --> 00:09:31,700
+فبيطلع منها clone، الـclone هذا ... الـclone يعني
+
+123
+00:09:31,700 --> 00:09:34,860
+السلالة كله نسخ عن مين؟ عن الـoriginal cell التي
+
+124
+00:09:34,860 --> 00:09:40,790
+صار لها activation الـclone بنقسم لقسمين، قسم بنسميه
+
+125
+00:09:40,790 --> 00:09:45,470
+memory cells وهذا بنقوله أنت خليك ما تسيرش أي 
+
+126
+00:09:45,470 --> 00:09:49,590
+development خليك صاحي، لو السنة الجاية اللي بعدها
+
+127
+00:09:49,590 --> 00:09:53,710
+نفس الـ antigen دخل، أنت بتشوف شغلك، الوزير الثاني
+
+128
+00:09:53,710 --> 00:09:58,690
+لأ بينضج إلى ما يسمى بـ plasma cells، الـ plasma
+
+129
+00:09:58,690 --> 00:10:02,490
+cells هي المنتجة من الـ antibodies، إذا الـ
+
+130
+00:10:02,490 --> 00:10:07,840
+antibodies تنتج من قبل plasma cells، أحنا اللي هو
+
+131
+00:10:07,840 --> 00:10:12,800
+هذه تلخيص سريع لكن هناخد شوية تفاصيل إن شاء الله زي
+
+132
+00:10:12,800 --> 00:10:15,980
+في اللوحة الثانية عندنا cells of the immune system
+
+133
+00:10:15,980 --> 00:10:20,020
+بقولنا myeloid و lymphoid، في الـ myeloid قسمناها
+
+134
+00:10:20,020 --> 00:10:24,480
+لقسمين granulocytic و monocytic، الـ granules اللي
+
+135
+00:10:24,480 --> 00:10:27,980
+فاكرينهم، الـ granulocytes اللي هم التلاتة الـ
+
+136
+00:10:27,980 --> 00:10:33,300
+neutrophil و الـ basophil و الـ eosinophil، طبعا صنفه
+
+137
+00:10:33,300 --> 00:10:40,040
+feel eosinophil أو basophil أو neutrophil حسب الـ acidity أو الـ
+
+138
+00:10:40,040 --> 00:10:45,350
+pH تبعها اللي بتدخّل، الـ monocytic في منها اللي هو
+
+139
+00:10:45,350 --> 00:10:48,930
+الـ macrophage و الـ ... الـ ... الـ Langerhans و الـ
+
+140
+00:10:48,930 --> 00:10:53,170
+Kupffer cells و الـ dendritic cells و الـ dendritic
+
+141
+00:10:53,170 --> 00:10:56,070
+cells، هتلاقي كلهم monocytic cells، يعني من الـ mono
+
+142
+00:10:56,070 --> 00:11:00,470
+cytes، من الـ monocytes، بينما الـ lymphoid الـ
+
+143
+00:11:00,470 --> 00:11:05,750
+lymphoid cells بتقسم زي ما تشوفوا إلى T cells و B
+
+144
+00:11:05,750 --> 00:11:09,490
+cells، وطبعا dendritic cells ساعتها على علامة استفهام
+
+145
+00:11:09,490 --> 00:11:13,770
+على أنه يعني under investigation أنها تكون تابعة
+
+146
+00:11:13,770 --> 00:11:17,990
+الـ lymphoid cells، و الـ T cells زي ما حكينا منها
+
+147
+00:11:17,990 --> 00:11:22,030
+اللي هو الـ cytotoxic T cell اللي هي الـ CD8 أو الـ
+
+148
+00:11:22,030 --> 00:11:25,890
+helper cell اللي هي الـ CD4، الـ B cells زي ما حكينا
+
+149
+00:11:25,890 --> 00:11:28,190
+بينبثق عنها الـ plasma cells
+
+150
+00:11:31,740 --> 00:11:37,980
+طيب الـ immune system could be classified based on
+
+151
+00:11:37,980 --> 00:11:44,020
+many variables، وحق الـ specificity أنه specific و
+
+152
+00:11:44,020 --> 00:11:49,200
+لا non specific، يعني هل بيقوم بوظيفته لإن هو عارف
+
+153
+00:11:49,200 --> 00:11:52,920
+هذا الـ antigen كيف بيشتغل و أنا كيف بتعامل معاه
+
+154
+00:11:52,920 --> 00:11:58,880
+ولا أي ... بشكل ... مش بشكل عجوز ... بشكل خلينا نسميه
+
+155
+00:11:58,880 --> 00:12:03,520
+غير متخصص ... غير متخصص ... دخل لسن ... اتعرفنا عليه
+
+156
+00:12:03,520 --> 00:12:06,160
+إن هو غريب ... انتهى الموضوع ... بنهاجمه ... كأي
+
+157
+00:12:06,160 --> 00:12:11,080
+إن المان كان ... أمام؟ و تحت الـ specific و non
+
+158
+00:12:11,080 --> 00:12:14,660
+specific، لجينا لأنواعين ... humoral و cellular ...
+
+159
+00:12:14,660 --> 00:12:18,420
+تعالوا نفهم إيش معنى humoral بالأول ... humoral
+
+160
+00:12:18,420 --> 00:12:22,670
+معناها سائل ... سائل يعني مناعة سائلة، إيش مناعة ...
+
+161
+00:12:22,670 --> 00:12:26,870
+مناعة سائلة؟ يعني غير خلوية، غير خلوية، ما فيش فيها
+
+162
+00:12:26,870 --> 00:12:30,810
+خلايا ولا ما فيش ممكن antibodies، ممكن complement،
+
+163
+00:12:30,810 --> 00:12:33,770
+ممكن proteins، non-proteins، component، لكن في
+
+164
+00:12:33,770 --> 00:12:37,310
+الآخر ما فيش إيه؟ خلايا، إيش بيسميها؟ humoral
+
+165
+00:12:37,310 --> 00:12:42,150
+immune، الثانية cellular immunity، طبعا واضح من الاسم
+
+166
+00:12:42,150 --> 00:12:47,170
+خلوية، بالدراسة وقتها نقطة عشان موضوع التصنيفات بعض
+
+167
+00:12:47,170 --> 00:12:53,050
+الأحيان بيحطنا في قلب معين، احنا لما بيصير عندنا ردة
+
+168
+00:12:53,050 --> 00:12:58,910
+فعل مناعية، هل بيصير عندنا cellular immunity ولا humoral
+
+169
+00:12:58,910 --> 00:13:01,990
+ولا الـ immunity، يعني ممكن بمعنى آخر إن واحدة
+
+170
+00:13:01,990 --> 00:13:06,310
+لحالها تصير والتانية ما تصيرش، غالبا التانين بيصيروا
+
+171
+00:13:16,720 --> 00:13:20,780
+نقسم البكتيريا إلى Intracellular و Extracellular
+
+172
+00:13:20,780 --> 00:13:23,960
+والـ Intracellular تسيبوا منها إنها أبلجات ولا
+
+173
+00:13:23,960 --> 00:13:30,360
+فاكولتات، في الآخر هي Intracellular، فـ Extracellular
+
+174
+00:13:30,360 --> 00:13:34,440
+أهم component في درجة المناعة الـ humoral innate
+
+175
+00:13:34,440 --> 00:13:37,420
+وأهم component في الـ humoral innate الانتيبادي
+
+176
+00:13:37,420 --> 00:13:41,200
+تمام؟ لما تكون البكتيريا عايشة
+
+177
+00:13:45,730 --> 00:13:52,570
+أهم component هو الـ cellular immunity
+
+178
+00:13:52,570 --> 00:13:57,190
+أو إلها اسم ثاني cell-mediated immunity، فاكرين
+
+179
+00:13:57,190 --> 00:14:03,430
+كلمة cytotoxic T cell و natural killer cell؟ هذول
+
+180
+00:14:03,430 --> 00:14:08,490
+بنتهيوا إيش معلومة دولة؟ طبقنا له خلايا، طب أي خلايا
+
+181
+00:14:08,490 --> 00:14:12,090
+بيقتلوا الخلايا المصابة، يبقى لما البكتيريا تكون
+
+182
+00:14:12,090 --> 00:14:17,490
+جوا داخل الخلية تبعنا أو الفيروس يكون، فبنقدرش
+
+183
+00:14:17,490 --> 00:14:21,370
+نقتل البكتيريا، فبنقتل مين احنا اللي هو الخلية
+
+184
+00:14:21,370 --> 00:14:25,030
+بالبكتيريا اللي فيها، إذا هيك الـ T cell أو الـ cell
+
+185
+00:14:25,030 --> 00:14:27,930
+mediated immunity بتكون كان الموضوع أهم
+
+186
+00:14:32,150 --> 00:14:34,830
+الميزان اللي موجود قدامك، وهذا على الصفر أو على
+
+187
+00:14:34,830 --> 00:14:37,350
+اللوحة هو الميزان اللي حكينا فيه من بداية
+
+188
+00:14:37,350 --> 00:14:42,090
+المحاضرات، الـ immunity أو الـ immunity الـ الـ
+
+189
+00:14:42,090 --> 00:14:44,510
+violence اللي هو الـ type of microorganism و الـ
+
+190
+00:14:44,510 --> 00:14:50,450
+number، بوراس معناها الجرعة أو الـ number، وهذول
+
+191
+00:14:50,450 --> 00:14:53,570
+التلات عناصر هم اللي بيحددوا إما الـ frequency of
+
+192
+00:14:53,570 --> 00:14:56,810
+disease أو حتى الـ severity of disease، إيش يعني
+
+193
+00:14:56,810 --> 00:15:01,930
+frequency؟ نسبة حدوثه أو شدته، تمام؟ شدة المرضى،
+
+194
+00:15:01,930 --> 00:15:08,690
+لكن مرارا وتكرارا أنا أكيد على مفهوم مهم جدا، وهو
+
+195
+00:15:08,690 --> 00:15:13,430
+إن الـ immunity لم نستطع قياسها بشكل دقيق لحد الآن،
+
+196
+00:15:13,430 --> 00:15:18,210
+وبالتالي هذه المعادلة يعني احنا ما نقدرش نحط محلها
+
+197
+00:15:18,210 --> 00:15:21,890
+أرقام، غالبا ما نحطش محلها أرقام، نعوض فيها و نحصل
+
+198
+00:15:21,890 --> 00:15:26,110
+على نسبة معينة، ولكن هي logical equation، يعني
+
+199
+00:15:26,110 --> 00:15:30,470
+خليني أقول معادلة منطقية أكثر من ما هي حساب، طيب
+
+200
+00:15:30,470 --> 00:15:34,710
+أهمية الجهاز المناعة، بدا منطلق لها من جانبين طبعا
+
+201
+00:15:34,710 --> 00:15:38,870
+لما أقول أهمية جهاز المناعة أكيد تلقائيا بيتبادل
+
+202
+00:15:38,870 --> 00:15:42,890
+لذهن الدور الإيجابي أصلا، لأ الدور الإيجابي في إنه
+
+203
+00:15:42,890 --> 00:15:48,810
+بيعملنا حماية من الغزاة الـ invaders، تمام؟ و بيعمل
+
+204
+00:15:48,810 --> 00:15:54,050
+حتى كمان قتل و destruction للخلايا اللي مش طبيعية،
+
+205
+00:15:54,050 --> 00:15:57,630
+يعني مثلا تكونت خلية سرطانية، بالك مين بيهاجمها
+
+206
+00:15:57,630 --> 00:16:01,070
+أو لا بيهاجمها؟ جهاز المناعة، تمام؟ فجهاز المناعة
+
+207
+00:16:01,070 --> 00:16:05,970
+إله دورين، بيحارب الغزاة، ولو صار في خلايا غريبة
+
+208
+00:16:05,970 --> 00:16:10,210
+جوا، يعني غريبة بمعنى آخر، نموها مش طبيعي، برضه
+
+209
+00:16:10,210 --> 00:16:16,120
+بيقتلها، لكن فيه كمان له تأثير ضعف، determinant، إيش
+
+210
+00:16:16,120 --> 00:16:21,400
+تأثيره الضعف؟ إنه الـ inflammation الناجمة عن شغله
+
+211
+00:16:21,400 --> 00:16:26,420
+لما بيشتغل، احنا بيعملوا مبنى عندنا في الجامعة، المبنى
+
+212
+00:16:26,420 --> 00:16:29,100
+كويس ولا ... لكن احنا قاعدين في المحاضرة تسمع
+
+213
+00:16:29,100 --> 00:16:34,380
+الصوت المزعج، مين مزعج؟ بما إنه بيكون بشيء إيجابي،
+
+214
+00:16:34,380 --> 00:16:37,700
+نفس الجهة، جهة المناعة، هو بيعمل الشيء الإيجابي
+
+215
+00:16:37,700 --> 00:16:43,540
+تبعه، قد يعني يسبب إنه discomfort، اللي أهم من
+
+216
+00:16:43,540 --> 00:16:50,080
+ذلك رقم اثنين، damage to self، طبعا الـ damage to
+
+217
+00:16:50,080 --> 00:16:54,400
+self احنا بنعرف إنه جهاز المناعة programmed إنه يتعرف
+
+218
+00:16:54,400 --> 00:16:57,960
+على خلايانا، عليها self، وعلى الـ others in a non
+
+219
+00:16:57,960 --> 00:17:03,440
+self، وهذا الطبيعي، كلنا الآن يعني لو جهاز المناعة
+
+220
+00:17:03,440 --> 00:17:06,140
+ما كانوش هذه القدرة كان ولا واحد فينا جالس في هذه
+
+221
+00:17:06,140 --> 00:17:06,680
+المحاضرة
+
+222
+00:17:09,360 --> 00:17:13,140
+طيب جهاز المناعة بيفشل في التمييز بين self و non
+
+223
+00:17:13,140 --> 00:17:16,660
+-self؟ نعم بيفشل، إيش الأسباب؟ ممكن تكون كتيرة، أه
+
+224
+00:17:16,660 --> 00:17:21,280
+يفتكر واحد منها إيه له علاقة بالـ microbiology، الآن
+
+225
+00:17:21,280 --> 00:17:26,900
+لو بكتيريا لبست، فاكرين الخروف، تمام؟ لو لبست
+
+226
+00:17:26,900 --> 00:17:32,040
+أنتيجينات أو عليها أنتجينات شبيهة بأنتيجيناتنا
+
+227
+00:17:32,040 --> 00:17:36,900
+احنا، والنفط العدل تلقالي، كلمة شبيهة مش مطابقة،
+
+228
+00:17:36,900 --> 00:17:41,450
+يعني متشابهة ببعض مش نسخة عن بعض، الآن جسم الإنسان
+
+229
+00:17:41,450 --> 00:17:44,890
+تعرف أو جهاز المناعة عرف على الـ antigen تبع
+
+230
+00:17:44,890 --> 00:17:49,450
+البكتيريا وكوّن أجسام مضادة، الأجسام المضادة للأسف
+
+231
+00:17:49,450 --> 00:17:53,670
+الـ specificity تبعها ما قدرت تتميز الفروقات اللي
+
+232
+00:17:53,670 --> 00:17:57,590
+بين الاثنين، فعملت أدات من تبع البكتيريا وعملت أدات
+
+233
+00:17:57,590 --> 00:17:58,090
+من تبعنا
+
+234
+00:18:02,680 --> 00:18:06,300
+من الـ antibodies بدل ما توجه ضد البكتيريا، ساسمين
+
+235
+00:18:06,300 --> 00:18:11,360
+لعدد القلب، طبعا هي بالنسبة للبكتيريا ميزة طبعا بدل
+
+236
+00:18:11,360 --> 00:18:15,160
+ما تضربوا عشرة واحد، بدل ما يكفي يعني بتتحملوا، لكن
+
+237
+00:18:15,160 --> 00:18:20,020
+بالنسبة للجسم أكيد بينضر، لذلك بتعرفوا أنتو
+
+238
+00:18:20,020 --> 00:18:24,440
+الالتهاب اللوز اللي هو tonsillitis streptococcus
+
+239
+00:18:24,440 --> 00:18:31,940
+virus عندها الحيلة هذه، فالطفل اللي بيصاب مرارا و
+
+240
+00:18:31,940 --> 00:18:37,040
+تكرارا بالـ tonsillitis ... كلكم بتسمعوا إنه بيحاول
+
+241
+00:18:37,040 --> 00:18:41,800
+إن أهله يعملوا له عملية إزالة لازم ... لكن اللي بتتكرر
+
+242
+00:18:41,800 --> 00:18:45,680
+عنده الإصابة بالـ tonsillitis قد يكون عمره 15 سنة
+
+243
+00:18:45,680 --> 00:18:50,780
+لكن قلبه عمره 80 سنة، المفاصلة تكون عمرها 60 سنة،
+
+244
+00:18:50,780 --> 00:18:54,460
+ليش؟ من الـ attack للـ antibodies ... طبعا الـ attack
+
+245
+00:18:54,460 --> 00:18:57,280
+للـ antibodies شو بيؤدي يا بنات؟ هو ارتباط الـ ...
+
+246
+00:18:57,280 --> 00:18:59,720
+الـ ... الـ antibody بالـ ... بالـ ... بالـ ... بالمكان مش
+
+247
+00:18:59,720 --> 00:19:04,080
+كثير يعني مضر، لكن الارتباط بيؤدي لـ activation للـ
+
+248
+00:19:04,080 --> 00:19:07,830
+complement، والكملة بتعمل destruction لأنسجة اللي
+
+249
+00:19:07,830 --> 00:19:11,210
+مرتبط عليها الـ antibody، فبيصير في destruction، فبيصير
+
+250
+00:19:11,210 --> 00:19:16,130
+في destruction وبالتالي بيظهر الأثر لذلك حتى وإن 
+
+251
+00:19:16,130 --> 00:19:19,050
+بلد التونسوليتس بقت صديقة إن احنا صغار يعني كل شهر
+
+252
+00:19:19,050 --> 00:19:23,410
+شهرين مصاب مرة واحنا صغار لكن ال frequency تبع
+
+253
+00:19:23,410 --> 00:19:27,890
+حين ميزيد بالفعل بتؤدي إلى مخاطر جسيمة واحدة
+
+254
+00:19:27,890 --> 00:19:31,150
+بيسموها ال complication أو ال sequella اللي هي
+
+255
+00:19:31,150 --> 00:19:37,910
+immunological والسبب فيها مين؟ صحيح البكتيريا سبب
+
+256
+00:19:37,910 --> 00:19:40,410
+triggering، لكن ال .. اللي .. اللي أنتج ال
+
+257
+00:19:40,410 --> 00:19:44,110
+antibody هو من بيسمنا، فالضرر من وين؟ من البيسمنا،
+
+258
+00:19:44,110 --> 00:19:48,610
+وهذا يعتبر برضه autoimmune، إذن هناك وقت .. أنا
+
+259
+00:19:48,610 --> 00:19:51,950
+في كمان ضرر ثالث مش مذكور على السبورة، بس ده كانت
+
+260
+00:19:51,950 --> 00:19:55,930
+الكوية، لما تكون رد فعل بجهة بعدية الملاعق غير
+
+261
+00:19:55,930 --> 00:20:00,210
+متناسبة مع حجم ال antigen، حكينا فيها زي ال super
+
+262
+00:20:00,210 --> 00:20:06,080
+antigen زي ما أحد يتخيل أنه عنده دبابة على إيده و
+
+263
+00:20:06,080 --> 00:20:12,920
+روحته خاب مسدس مثلًا تمام؟ مناسبة لرد الفعل؟ هتحدث
+
+264
+00:20:12,920 --> 00:20:17,100
+ضرر في ضرر؟ هتحدث ضرر إذا لما تكون ال exaggerated
+
+265
+00:20:17,100 --> 00:20:20,120
+immune response بالفعل يكون في ضرر في كمان ضرر
+
+266
+00:20:20,120 --> 00:20:25,940
+رابع اللي هو ال hypersensitivity واحد جَرْسَة نحلة و
+
+267
+00:20:25,940 --> 00:20:29,140
+خلال .. خلال ربع ساعة ونصف ساعة انتفخ وصار عنده
+
+268
+00:20:29,140 --> 00:20:33,200
+احمرار وممكن الدور الدموي يفشل في ال failure و
+
+269
+00:20:33,200 --> 00:20:37,440
+يموت هذا اسمه immediate type hypersensitive هذا رد
+
+270
+00:20:37,440 --> 00:20:42,320
+فعل مناعي برضه جهاز المناعة مسئول عنها تمام؟ إذا 
+
+271
+00:20:42,320 --> 00:20:46,420
+هناك ب .. ب .. بمقابل الفوائد اللي بنجنها من جهاز
+
+272
+00:20:46,420 --> 00:20:50,160
+المناعة أيضًا هناك ايه؟ مضار ممكن تحدث
+
+273
+00:20:55,080 --> 00:20:59,860
+تقسيمة ثانية adaptive and innate immunity أنا بدأت
+
+274
+00:20:59,860 --> 00:21:03,540
+أقول لكم بيها بكلمة بسيطة ال innate ما بتتعلمش ال
+
+275
+00:21:03,540 --> 00:21:04,960
+adaptive بتتعلم
+
+276
+00:21:07,140 --> 00:21:10,180
+ليش بتتعلمش ال .. ال .. ال innate؟ لأنه ما عندهوش
+
+277
+00:21:10,180 --> 00:21:13,440
+memory ما عندهوش memory يعني زي واحد نقعد نشرح له
+
+278
+00:21:13,440 --> 00:21:16,980
+مائة مرة microbiology وما عندهوش ذاكرة ونقول له بيدي
+
+279
+00:21:16,980 --> 00:21:21,200
+امتحان، بينجح؟ ما بينجحش ولا عمره قدر ولا عصر .. يمكن
+
+280
+00:21:21,200 --> 00:21:23,600
+ينسى الفصل .. ينسى المكان اللي بيروي عليه في
+
+281
+00:21:23,600 --> 00:21:28,520
+المحاضرة أساسًا، ما عندهوش .. ما عندهوش ذاكرة فجهاز
+
+282
+00:21:28,520 --> 00:21:31,660
+المناعة .. جهاز المناعة .. جهاز المناعة بيقسم إلى
+
+283
+00:21:31,660 --> 00:21:35,930
+قسمين هذا لا يعني أن أنا بأقلل من قيمة ال innate
+
+284
+00:21:35,930 --> 00:21:40,810
+immune system بيقول بتتعلمش لأ لكن أنا بأقول لكم
+
+285
+00:21:40,810 --> 00:21:45,050
+بأوصف لكم حالته إن هو بالفعل ما بيتعلمش بينما ال
+
+286
+00:21:45,050 --> 00:21:48,070
+adaptive immune system لإنه في عنده memory آخر
+
+287
+00:21:48,070 --> 00:21:52,870
+واحدة تلاتينا development of memory معناها الحاجة
+
+288
+00:21:52,870 --> 00:21:57,230
+إنها بتتعلم زي ما قلنا قبل شوية احنا بنقسم الخلايا
+
+289
+00:21:57,230 --> 00:21:59,530
+خلايا بتشتغل بتتحول ل plasma cell وخلايا أخرى تتحول
+
+290
+00:21:59,530 --> 00:22:04,500
+لمية ل memory cell بيها بيتعلم وبيتذكر وبالتالي لما
+
+291
+00:22:04,500 --> 00:22:08,500
+ال microbe تقول كده مرة احنا we are ready وكتير من
+
+292
+00:22:08,500 --> 00:22:11,920
+الأمراض المعدية اللي كانت زمان قاتلة أصبحت غير
+
+293
+00:22:11,920 --> 00:22:19,040
+قادرة أعطيكم بس مثال بسيط كانت
+
+294
+00:22:19,040 --> 00:22:24,220
+لما الحصبة تمر في حارة .. حارة يعني منطقة تمام؟
+
+295
+00:22:24,220 --> 00:22:29,910
+تقتل نصف الأطفال اللي مولودين في المنطقة مرة تدخل
+
+296
+00:22:29,910 --> 00:22:34,910
+على منطقة معينة ممكن تتصل فيها لعشرين أو تلاتين في
+
+297
+00:22:34,910 --> 00:22:41,290
+المية لما ال smallpox كان يصيب منطقة كانت نسبة
+
+298
+00:22:41,290 --> 00:22:44,570
+القتل والدمار والتشوّه اللي ناتج عنه بقوة كونه
+
+299
+00:22:44,570 --> 00:22:49,470
+عالية جدًا كل هذه الأمراض مُنِعت مُنِعة أو شبه
+
+300
+00:22:49,470 --> 00:22:55,030
+مُنِعة بال vaccination ايش ال vaccination؟ memory
+
+301
+00:22:56,050 --> 00:23:00,610
+أنا عرضت جهاز المناعة لل antigen فكون memory
+
+302
+00:23:00,610 --> 00:23:04,290
+وبالتالي يمكن كل يوم بجي أدخل أنا بيروسات حصبة
+
+303
+00:23:04,290 --> 00:23:12,850
+وما أنصبناش لأن في عندنا من يكافح اللي هو الحصبة نفس
+
+304
+00:23:12,850 --> 00:23:18,850
+الجثة هو إن أنا في الآخر بأعطي immunity و memory مع
+
+305
+00:23:18,850 --> 00:23:24,050
+ال immunity طيب إن أنا أعمل برضه فروقات الأول
+
+306
+00:23:28,620 --> 00:23:34,280
+antigen dependent كل جملة بنحكيها تؤدي لورقها في
+
+307
+00:23:34,280 --> 00:23:37,220
+الآخر مادام antigen dependent تبقى ال memory نجحت
+
+308
+00:23:37,220 --> 00:23:44,720
+من هذه القصة ال lag period كونه عملية ذكية يبقى
+
+309
+00:23:44,720 --> 00:23:48,240
+فيها عملية تخطيط فيها عملية تخطيط وعملية التخطيط
+
+310
+00:23:48,240 --> 00:23:53,140
+بتأخذ budget أنا لما بأجي بأخذ action بقعد مثلًا نص
+
+311
+00:23:53,140 --> 00:23:58,330
+ساعة ساعة بكتب جدول وبعدين ببجي أتكلم لكن إذا أنا
+
+312
+00:23:58,330 --> 00:24:01,290
+جاهز، خلاص، انتهينا من الموضوع، نبدأ من البداية
+
+313
+00:24:01,290 --> 00:24:04,810
+جهاز المناعة الأولاني اللي هو الـ Adaptive ما فيش
+
+314
+00:24:04,810 --> 00:24:09,110
+عنده أي فترة جاهز لأنه مجهز لهذه العملية، ما بيضطرش
+
+315
+00:24:09,110 --> 00:24:12,250
+يفهم، بس نفس، تمام؟ بينما الجهاز التاني بيفهم،
+
+316
+00:24:12,250 --> 00:24:16,590
+فلازم يفكر ويعمل إجراءاته ثاني، فبالتالي في فترة
+
+317
+00:24:16,590 --> 00:24:19,890
+زمنية ما بين دخول الميكروب وبين ظهور اللي هو ال
+
+318
+00:24:19,890 --> 00:24:24,470
+effect تبع المناعة Antigenic specific within
+
+319
+00:24:24,470 --> 00:24:31,240
+development of memory طيب نجي لمقارنة بين ال
+
+320
+00:24:31,240 --> 00:24:34,740
+adaptive و ال innate immune system بس المرة هادي
+
+321
+00:24:34,740 --> 00:24:38,920
+بناء على العناصر المكوّنة لكل منهما العناصر الأول
+
+322
+00:24:38,920 --> 00:24:42,340
+ال physical barriers barriers معناها حواجز فهو
+
+323
+00:24:42,340 --> 00:24:48,420
+حواجز فيزيائية skin, gut, villi اللي هي ال villi
+
+324
+00:24:48,420 --> 00:24:53,460
+الموجودة في ال intestine ال lung و ال cilia
+
+325
+00:24:54,360 --> 00:24:59,580
+هذه كلها عناصر physical موجودة في ال innate immune
+
+326
+00:24:59,580 --> 00:25:05,720
+system طول عمرنا الجلد تبعنا بنجرح وبيغتأم طول
+
+327
+00:25:05,720 --> 00:25:11,680
+عمرنا جلدنا موجود وممنوع البكتيريا تدخل جوا، إذا 
+
+328
+00:25:11,680 --> 00:25:14,680
+كيف تدخل جوا البكتيريا؟ لما يصير فيه خدش
+
+329
+00:25:19,690 --> 00:25:24,130
+الجلد لبعض الأحيان مش شايفين إنه جرح لكن واقعي
+
+330
+00:25:24,130 --> 00:25:28,450
+الأمر هو صار مجروح لكن مش .. خلّيني أقول مصطلح
+
+331
+00:25:28,450 --> 00:25:33,450
+ثاني مش intact skin يعني مش متكامل فال skin موجود
+
+332
+00:25:33,450 --> 00:25:36,550
+physical barrier مهم جدًا في ال innate immune
+
+333
+00:25:36,550 --> 00:25:39,610
+system طبعًا في عوامل أخرى يعني في عالم اللي أنا
+
+334
+00:25:39,610 --> 00:25:43,030
+هأدرسوها إن شاء الله زي ال gastric acid على سبيل
+
+335
+00:25:43,030 --> 00:25:46,610
+المثال في إن الشمع اللي مرود في الأذن كمان يعتبر
+
+336
+00:25:47,150 --> 00:25:51,110
+Innate immunity، الـ adaptive immunity ما فيها
+
+337
+00:25:51,110 --> 00:25:55,850
+physical barriers ما فيها physical barriers،
+
+338
+00:25:55,850 --> 00:26:01,490
+soluble factors في ال innate immunity many soluble
+
+339
+00:26:01,490 --> 00:26:06,970
+أو many proteins و non-protein factors، بينما في
+
+340
+00:26:06,970 --> 00:26:10,800
+ال adaptive immunity فيه عندي ال immunoglobulins و
+
+341
+00:26:10,800 --> 00:26:15,600
+خاصة اللي ذكرناه ال IGA فاكرينه ال IGA اللي قلنا
+
+342
+00:26:15,600 --> 00:26:18,740
+سكراتوري أو solvigol وبروح على ال secretions
+
+343
+00:26:18,740 --> 00:26:20,860
+بنلاقيه في اللعاب بنلاقيه في ال nose بنلاقيه في ال
+
+344
+00:26:20,860 --> 00:26:23,980
+vagina بنلاقيه في أناكن فيها mucous membrane في
+
+345
+00:26:23,980 --> 00:26:26,680
+التراكيا بنلاقيه لما هتكلمنا عن البكتيريا اللي
+
+346
+00:26:26,680 --> 00:26:32,340
+تنتج IGA proteases طيب ال cells اللي موجودة في
+
+347
+00:26:32,340 --> 00:26:36,320
+الجهازين واحد ال phagocytes و ال natural killer و
+
+348
+00:26:36,320 --> 00:26:40,730
+ال eosinophil و ال K cells بينما في ال adaptive
+
+349
+00:26:40,730 --> 00:26:44,930
+immunity لاحظوا معتمدة علميًا على T و B lymphocytes
+
+350
+00:26:44,930 --> 00:26:51,750
+إذا بالفعل ال adaptive immunity معتمدة على ال
+
+351
+00:26:51,750 --> 00:26:56,650
+immunoglobulins وعلى ال T و ال B cells إذا طب
+
+352
+00:26:56,650 --> 00:27:00,530
+تعرف تميز ال adaptive immunity إلى قسمين رئيسي
+
+353
+00:27:00,530 --> 00:27:00,770
+يعني
+
+354
+00:27:05,200 --> 00:27:08,900
+بتحاول تلتقط اشريشك والله يلاحظوا إن ال size of ال
+
+355
+00:27:08,900 --> 00:27:13,660
+macrofage ضخم بالنسبة للبكتيريا فتستطيع في حركة
+
+356
+00:27:13,660 --> 00:27:17,460
+واحدة التقال أكثر من بكتيريا فالها كفاءة يعني اللي
+
+357
+00:27:17,460 --> 00:27:20,500
+هم استطاعوا إن يقللوا من أهميتها في جهاز المناعة
+
+358
+00:27:20,500 --> 00:27:23,960
+هذا
+
+359
+00:27:23,960 --> 00:27:30,620
+الصورة اللي قدامنا بدنا نطلع عليها ونشوف نقطة
+
+360
+00:27:30,620 --> 00:27:31,200
+كثير مهمة
+
+361
+00:27:34,510 --> 00:27:41,210
+ياريت تركزوا على الخلية المصّوفة بشكل عربي،
+
+362
+00:27:41,210 --> 00:27:46,510
+فلنعرف إن ال blood flows in vessels، في أوعية،
+
+363
+00:27:46,510 --> 00:27:51,050
+نسميها الأوعية الدموية، إما تكون شرايين أو أوردة أو
+
+364
+00:27:51,050 --> 00:27:57,030
+كابيلارس اللي بتوصل بين الشرايين والأوردة، الآن لما بيكون في
+
+365
+00:27:57,030 --> 00:28:02,940
+stimuli، كلمة stimuli وكلمة .. خلّينا ما ندي بصراحة
+
+366
+00:28:02,940 --> 00:28:05,780
+نتكلم عن infection نتكلم عن مصطلح ثاني اسمه
+
+367
+00:28:05,780 --> 00:28:11,000
+inflammation بالعربي الالتهاب غالبًا referred to as
+
+368
+00:28:11,000 --> 00:28:14,620
+التهابات وواحد عنده inflammation أو عنده عدوى
+
+369
+00:28:14,620 --> 00:28:18,080
+بنسميها التهابات لكن العدوى هي التهاب العدو هي
+
+370
+00:28:18,080 --> 00:28:21,720
+التهاب لأن ليس كل التهاب هو عدوى فال inflammation ..
+
+371
+00:28:21,720 --> 00:28:24,460
+ال .. ال .. ال infection inflammation بس مش كل
+
+372
+00:28:24,460 --> 00:28:28,280
+inflammation هو .. فال inflammation هو response لل
+
+373
+00:28:28,280 --> 00:28:31,800
+.. من الجسم ل injury وكلمة injury ممكن تفهمها جرح
+
+374
+00:28:31,800 --> 00:28:35,880
+بالسكين ممكن لو احنا انكبنا على ماء ساخنة هذا
+
+375
+00:28:35,880 --> 00:28:40,300
+injury لو احنا دخل بكتيريا أو فيروسات في الجسم هذا 
+
+376
+00:28:40,300 --> 00:28:45,120
+injury chemicals ممكن تكون يعني أي عامل يحدث ضرر
+
+377
+00:28:45,120 --> 00:28:50,520
+بجسم اللي بكوّنه استجابة بيسميها inflammation من
+
+378
+00:28:50,520 --> 00:28:54,500
+ضمن ال inflammation بعض الأحيان ما يسمى بـ ال ..
+
+379
+00:28:54,500 --> 00:28:58,000
+الـ .. الـ .. الـ .. الـ acute phase proteins
+
+380
+00:28:58,000 --> 00:29:02,700
+phase proteins حيمرّ معانا ذكرها في .. للـ .. لـ ..
+
+381
+00:29:02,700 --> 00:29:06,940
+لطلبات سنة تانية لما .. نفتقر شوية سنة لما ندرسوا
+
+382
+00:29:06,940 --> 00:29:10,020
+المناعة هتعرفوا إيش هي الـ acute phase protein بـ ..
+
+383
+00:29:10,020 --> 00:29:14,240
+بـ .. بتفاصيل كبيرة إن شاء الله لكن بنعرف إنه كمان
+
+384
+00:29:14,240 --> 00:29:19,370
+فيها اسمها cytokines هي الـ messengers و 
+
+385
+00:29:19,370 --> 00:29:24,130
+أنا بدي أنبّهكم لنقطة معينة، ممكن أنا أكون الـ
+
+386
+00:29:24,130 --> 00:29:27,950
+messenger  أن أقول لك نالي عفوًا، لإنّها هاي الـ
+
+387
+00:29:27,950 --> 00:29:30,890
+messenger  إنّه واصلك أنت to activate another
+
+388
+00:29:30,890 --> 00:29:35,480
+cell to produce another cytokine فالعملية معقدة
+
+389
+00:29:35,480 --> 00:29:38,340
+ليس عملية سهلة، وكل يوم نكتشف .. مش كل يوم يا ربنا
+
+390
+00:29:38,340 --> 00:29:43,640
+في فهم .. في فهم يوميًا، لأ كل سنة نكتشف واحد أو
+
+391
+00:29:43,640 --> 00:29:48,080
+اثنين وحتى على مستوى الخلايا حتى نعرف أنواع خلايا
+
+392
+00:29:48,080 --> 00:29:52,000
+ما كناش نعرفها من الـ immune system الـ immune system
+
+393
+00:29:52,000 --> 00:29:56,380
+قائم على .. قائم على messages و very specific
+
+394
+00:29:56,380 --> 00:30:01,020
+messages أي خلل فيها .. أي خلل فيها بيضعف جهاز
+
+395
+00:30:01,020 --> 00:30:04,080
+المناعة، عشانك أنا قلت لكم دائما وأبدا يصعب جهاز
+
+396
+00:30:04,080 --> 00:30:08,120
+.. قياس جهاز المناعة إذا واحد مش فاهم الجهاز كله
+
+397
+00:30:08,120 --> 00:30:15,580
+كبدي جسدي بيكون صعب طيب صار في injury بـ .. بـ .. بـ
+
+398
+00:30:15,580 --> 00:30:19,860
+.. بـ بكتيريا صار في cytokines طلعت و نالتني الآن
+
+399
+00:30:19,860 --> 00:30:24,060
+الـ neutrophil اللي هو الموجودة في الدم
+
+400
+00:30:26,890 --> 00:30:31,010
+نفس الـ cytokine بيعمل حزمة of vasodilatation،
+
+401
+00:30:31,010 --> 00:30:40,160
+توسيع للأوعية الدموية عشان تقدر حضرة الـ netrophil
+
+402
+00:30:40,160 --> 00:30:47,300
+إنها تخرج من الدم إلى الأنسجة الأنسجة اللي مصابة
+
+403
+00:30:47,300 --> 00:30:52,520
+بعملية بيسميها diapedesis، diapedesis اللي هي خروج
+
+404
+00:30:52,520 --> 00:30:56,760
+كمان مرة الـ fagocytes صاعد من الدم أو الـ blood vessels
+
+405
+00:30:56,760 --> 00:30:58,220
+إلى الأنسجة
+
+406
+00:31:03,270 --> 00:31:08,770
+كمان مره ثانية معناها الكلمة خروج الـ fagocytes من الـ
+
+407
+00:31:08,770 --> 00:31:13,390
+blood vessels إلى الأنسجة المصابة وطبعًا خروجها مش
+
+408
+00:31:13,390 --> 00:31:21,230
+عشوائي وإنما نتيجة اللي هو stimuli الـ
+
+409
+00:31:21,230 --> 00:31:35,830
+adaptive immunity اللي اسمها تتعلم صح؟ وهي specific
+
+410
+00:31:35,830 --> 00:31:40,810
+and is mediated either by antibody or lymphoid
+
+411
+00:31:40,810 --> 00:31:49,190
+cell it can be active or it can be passive مهارة
+
+412
+00:31:49,190 --> 00:31:56,490
+الماضية قلنا لكم لو واحد يعني تعرض للضّغاء من ثعبان
+
+413
+00:31:58,110 --> 00:32:02,770
+السمّ اللي موجود الآن في الدم مصل الدم ما فيش عندي
+
+414
+00:32:02,770 --> 00:32:08,110
+وسيلة إنّ أنا أعمله اه أنجل دمه برة وأشيل السمّ منه
+
+415
+00:32:08,110 --> 00:32:12,190
+فوسيلة الأولى اللي بنلجأ لها إن يكون عندنا أجسام
+
+416
+00:32:12,190 --> 00:32:15,550
+مضادة وكل دولة في العالم بتبقى متعارفة على
+
+417
+00:32:15,550 --> 00:32:18,190
+الثعابين اللي عندها في الحياة والعقارب والعناكب
+
+418
+00:32:18,190 --> 00:32:24,890
+السامة وعملة antibodies جاهزة في vials الطريقة
+
+419
+00:32:24,890 --> 00:32:28,970
+لعلاج الإنسان المصاب بالضّغاء إن you were thabab
+
+420
+00:32:28,970 --> 00:32:32,930
+إذا جينا نحصل الذّغاب الطبيب يتعرف عليه يعطيه الـ
+
+421
+00:32:32,930 --> 00:32:36,970
+antivenom لإنّ الـ antibody specific هنا بنعطي
+
+422
+00:32:36,970 --> 00:32:40,250
+antibiotism جاهزة هذا passive ولا active؟ passive
+
+423
+00:32:40,250 --> 00:32:44,850
+إنّ أنا ما .. ماخلطوش يطمحش أعطي جاهزة البناء وإنما
+
+424
+00:32:44,850 --> 00:32:50,030
+أعطيت حاجة جاهزة لكن هذا حلّ سريع ووحيد في هذه
+
+425
+00:32:50,030 --> 00:32:52,790
+الحالة لإنّ أنا بقدرش إنّ أنا أصلا نعطيه vaccine
+
+426
+00:32:52,790 --> 00:33:00,070
+لإنّه أصلا بيكون قام بدوره بينما لما أعطيته أنا
+
+427
+00:33:00,070 --> 00:33:02,990
+vaccine زي التركسويدز اللي حكيت عنه قبل شوية هذا
+
+428
+00:33:02,990 --> 00:33:07,140
+يتبع ليه إيش؟ active immune أجرب لكم يا أهل الأذهان
+
+429
+00:33:07,140 --> 00:33:11,340
+احنا مرات أنا بكون طالع مع صديق ومراهنين مشوار
+
+430
+00:33:11,340 --> 00:33:16,960
+ بسيارة هو اللي سايق أنا بكون relaxed خالص ولا
+
+431
+00:33:16,960 --> 00:33:21,100
+بتأثر ولا بانتبه للطرق ولو بدرجع مرة تانية على
+
+432
+00:33:21,100 --> 00:33:25,220
+نفس المكان ما بعرفش ليش؟ لإنّه مش أنا اللي بدّرت
+
+433
+00:33:25,220 --> 00:33:30,240
+الجهد فيه أنا وصليني جاهز بس لو العكس أنا اللي سوقت
+
+434
+00:33:30,240 --> 00:33:33,820
+ورحت وعرفت كل المناطق اللي طلعت المرة اللي جايش
+
+435
+00:33:33,820 --> 00:33:39,410
+بيهابكون اتعلمت فالـ passive الـ passive بيكون
+
+436
+00:33:39,410 --> 00:33:43,330
+already جاهز برة بينما الـ active احنا بنعمل
+
+437
+00:33:43,330 --> 00:33:47,410
+challenging للشخص أو للمريض إن يكون هو الأجسام
+
+438
+00:33:47,410 --> 00:33:57,510
+الموجودة طب ولو بكتيري
+
+439
+00:33:57,510 --> 00:34:02,720
+ولو طبيعي؟ يعني .. يعني أنت اللي بتتكلم عنه الـ
+
+440
+00:34:02,720 --> 00:34:06,060
+vaccine طب لو احنا انصبنا بـ .. بـ .. بـ .. ب فيروس
+
+441
+00:34:06,060 --> 00:34:09,900
+أو ببكتيريا معينة مش برضه هي بمثابة الـ antigen
+
+442
+00:34:09,900 --> 00:34:14,100
+اللي لازم نتعرف عليه و .. و يكوّنه بس الفرق في الـ
+
+443
+00:34:14,100 --> 00:34:19,120
+vaccine إنّه ما نمرضش بينما في العدوى العادية بنمرض
+
+444
+00:34:19,120 --> 00:34:23,480
+أما في المحصلة النهائية إنّه تحت أنت منكون منع و
+
+445
+00:34:23,480 --> 00:34:29,920
+تصوّره أو Artificial Artificial بتكون بالـ Vaccine و
+
+446
+00:34:29,920 --> 00:34:35,260
+Natural بتكون بالـ Infection طيب اللوحة اللي مدّنا
+
+447
+00:34:35,260 --> 00:34:41,260
+هذه يعني تلخيص لبعض الـ pathogens زي البكتيريا والـ
+
+448
+00:34:41,260 --> 00:34:46,200
+viruses و foreign proteins و fungi كثير منحصرة فانا
+
+449
+00:34:46,200 --> 00:34:50,540
+للأسف يعني مضطر إنّه أستخدم الصّبورة على أساس إنّه
+
+450
+00:34:50,540 --> 00:34:56,580
+نوضّح بعض النقاط إن شاء الله المتعلقة بالـ إنّه في
+
+451
+00:34:56,580 --> 00:35:00,540
+هنا منطقة خالية الصحية في البلاد اللي ممكن نتعرف
+
+452
+00:35:00,540 --> 00:35:04,840
+عليها فاكرين حد اسمه macrophage؟ إيش سمّينا المرة
+
+453
+00:35:04,840 --> 00:35:10,520
+الماضية هنا؟ wise
+
+454
+00:35:10,520 --> 00:35:14,960
+man الراجل الحكيم ليش اسمه الراجل الحكيم؟ لإنّه هي
+
+455
+00:35:14,960 --> 00:35:20,740
+اللي بتعمل عملية phagocytosis وبعدين بتقرّر لما
+
+456
+00:35:20,740 --> 00:35:26,330
+تكسر البكتيريا بتقرّر مين من الانتيجينات .. مين من
+
+457
+00:35:26,330 --> 00:35:30,490
+الانتيجينات تمام؟ اللي موجودة على أسطح البكتيريا
+
+458
+00:35:30,490 --> 00:35:35,330
+أو الفيروس بتعمله presentation فبتمسك انتيجين أو
+
+459
+00:35:35,330 --> 00:35:39,830
+أكثر وبتعملهم presentation على السطح هذه حركتها
+
+460
+00:35:39,830 --> 00:35:44,570
+بطيئة حركتها بطيئة فبطولة بتدور على مين .. على
+
+461
+00:35:44,570 --> 00:35:50,730
+حركتهم نشيطة .. مين حركتهم نشيطة؟ الـ T cells الآن
+
+462
+00:35:50,730 --> 00:35:54,980
+مش أي T cell أنا مش قادر أقول كل شيء specific في
+
+463
+00:35:54,980 --> 00:35:57,620
+جهاز المناعة، كل شيء specific، ما فيش أي T cell
+
+464
+00:35:57,620 --> 00:36:03,540
+بتروح الـ macrophage على تجمعات T cells وبتطلّع مين
+
+465
+00:36:03,540 --> 00:36:10,860
+عندها receptor بيقابل الـ antigen تبعنا ممكن تحتك
+
+466
+00:36:10,860 --> 00:36:16,860
+فيهم وتقرأهم وفي الآخر تقرّر أي T cell
+
+467
+00:36:22,560 --> 00:36:26,900
+الـ Receptor أو عفوًا الـ Antigen representation
+
+468
+00:36:26,900 --> 00:36:35,680
+مناسب إليها بينتقل على طول هذا إلى الـ T cell الـ
+
+469
+00:36:35,680 --> 00:36:39,560
+T cell لنا خفيفة ونشيطة أسرع من مين؟ من الـ macro
+
+470
+00:36:39,560 --> 00:36:47,180
+phage بتروح تدور على مين؟ على الـ B cell المناسبة
+
+471
+00:36:47,180 --> 00:36:52,460
+يبقى الـ macrophage أكلت البكتيريا عامة
+
+472
+00:36:52,460 --> 00:36:56,300
+الفيروسات أو الفيروس أو whatever وعامة ما يسمى
+
+473
+00:36:56,300 --> 00:37:00,360
+كما مرّت Antigen Presentation اللي ما بيعرفش إيش هالـ
+
+474
+00:37:00,360 --> 00:37:02,740
+Antigen Presentation بيصفّره على اللي يومين هدول
+
+475
+00:37:02,740 --> 00:37:09,260
+فترة دقسة بس يكون الخلية بيسمّوها مرات APC Antigen
+
+476
+00:37:09,260 --> 00:37:15,040
+Presenting Cell نظرًا للدور العظيم المناطق دي هذه
+
+477
+00:37:15,040 --> 00:37:21,200
+الخلية من إظهار للـ Antigenات تبعها البيتين بأصول
+
+478
+00:37:21,200 --> 00:37:25,690
+مين اللي احنا؟ للـ B cell الـ B cell هي على الصبورة
+
+479
+00:37:25,690 --> 00:37:31,890
+اللوحة ثاني بتطلّع what is next الـ B cell في هذه
+
+480
+00:37:31,890 --> 00:37:38,550
+الحالة اللي قبلته شاملة الـ B cell تخيّل واحد بينتظر
+
+481
+00:37:38,550 --> 00:37:43,330
+حدث مهم في حياته لن يقوم بيه إلا مرة واحدة وإيجاه
+
+482
+00:37:43,330 --> 00:37:50,130
+هذا الخبر ارتباط الانتيجين بالـ B-cell وكأنه Mitogen
+
+483
+00:37:50,130 --> 00:37:53,230
+Mitogen فاكرين إيش يعني Mitogen؟ بلحّ إنّ أنا أقول إنّ
+
+484
+00:37:53,230 --> 00:37:58,430
+الـ Mitogen هو compound بيعمل stimulation للـ cell
+
+485
+00:37:58,430 --> 00:38:03,430
+division فالانتيجين بمجرّد إنّه مرتبط بالـ B-cell تبدأ
+
+486
+00:38:03,430 --> 00:38:08,220
+الـ B-cell تنقسم مفتاح سمّينا ووصفنا كإنّه يقول لا
+
+487
+00:38:08,220 --> 00:38:13,540
+وكأنه ماتش، إذا بدأت الخلية تنقسم وقلنا بتنقسم إلى
+
+488
+00:38:13,540 --> 00:38:17,360
+B cells وإلى B cells، لكن B cells سمّينا هم memory
+
+489
+00:38:17,360 --> 00:38:23,740
+cells والثانية سمّينا هم لا، نظرًا للولاحظوا الحجم تبع
+
+490
+00:38:23,740 --> 00:38:28,560
+الـ plasma cell أكبر بكثير من مين من الـ B cells الـ
+
+491
+00:38:28,560 --> 00:38:33,780
+memory cells حجمها بلايه بيضلوا نفس الحجم تبع اللي
+
+492
+00:38:33,780 --> 00:38:37,980
+هو الـ B cells بينما الـ plasma cell طبعًا إنّها بدأت
+
+493
+00:38:37,980 --> 00:38:43,600
+تتكون بروتينات الـ minoglobulins الـ antibodies من
+
+494
+00:38:43,600 --> 00:38:51,370
+صنف واحد الـ B-cell الواحدة اللي تكاثرت إلى عشرات
+
+495
+00:38:51,370 --> 00:38:55,810
+أو مئات كلها هتنتج نفس الصنف من مين من الـ
+
+496
+00:38:55,810 --> 00:39:01,890
+antibodies لذلك B-clone, clonal, monoclonal تمام؟
+
+497
+00:39:01,890 --> 00:39:05,310
+إذا أكثر من B-cell اللي اشتغلت الـ response بيكون
+
+498
+00:39:05,310 --> 00:39:10,890
+polyclonal طب عندنا نقطة هذه على فكرة إنّها مهمة جدًا
+
+499
+00:39:10,890 --> 00:39:14,350
+بالنسبة لطلابنا يعرفوها الفرق بين monoclonal و
+
+500
+00:39:14,350 --> 00:39:15,290
+polyclonal
+
+501
+00:39:18,740 --> 00:39:22,860
+طبعا التخيل اللي كنتوا بتفكروا فيه عن الأنتيجين أنه هو قطعة
+
+502
+00:39:22,860 --> 00:39:28,740
+واحدة صح؟ كبرنا اليوم احنا صرنا في الجامعة مش في 
+
+503
+00:39:28,740 --> 00:39:33,720
+التنوية العامة لازم نعرف أن الأنتيجين الواحد قد
+
+504
+00:39:33,720 --> 00:39:38,300
+يكون أو يحمل قد يحمل أكثر من ما يسمى Epitope
+
+505
+00:39:38,300 --> 00:39:43,580
+Epitope يعني بيسموه Antigenic determinants
+
+506
+00:39:43,580 --> 00:39:47,900
+الأنتيجين الواحد بيبقى فيه أكثر من Epitope  أكثر من
+
+507
+00:39:47,900 --> 00:39:55,640
+إيه؟ من Epitop طيب كل Epitop بينشأ له antibody مثل
+
+508
+00:39:55,640 --> 00:39:58,520
+الأنتيجين الواحد بينشأ له يعني لو الأنتيجين الواحد
+
+509
+00:39:58,520 --> 00:40:04,260
+فيه أربع Epitopes كم antibody بيطلع له؟ أربع طيب لو
+
+510
+00:40:04,260 --> 00:40:08,140
+عندي محلول .. محلول فيه أربع أنواع من الـ antibodies
+
+511
+00:40:08,140 --> 00:40:12,040
+بقدر أطلع له monoclonal يا بنات؟ لا بقدر أطلع له 
+
+512
+00:40:12,040 --> 00:40:16,890
+monoclonal لأن هو محتوي على أربع أصناف من الـ
+
+513
+00:40:16,890 --> 00:40:20,950
+antibodies وكل من الأربع يانيبولي .. أربع
+
+514
+00:40:20,950 --> 00:40:25,730
+يانيبولي .. بس لما يكون صنف واحد بنسميه monoclonal
+
+515
+00:40:25,730 --> 00:40:30,390
+طب إيش أهمية الـ monoclonal في حياتنا؟ احنا كأطباء
+
+516
+00:40:30,390 --> 00:40:34,590
+علوم طبية مخبرية، عارفين إن هذا أحدث ثورة في الـ
+
+517
+00:40:34,590 --> 00:40:38,940
+diagnostics؟ الـ specificity إيش هي الـ specificity
+
+518
+00:40:38,940 --> 00:40:44,300
+التخصصية إيش هي التخصصية يعني لو أنا فعلت شيء مع
+
+519
+00:40:44,300 --> 00:40:48,860
+شيء الأصل if هذا specific ما يتفعل إلا مع الشيء
+
+520
+00:40:48,860 --> 00:40:55,420
+اللي بديه يعني صح هذا الـ specificity طبعا احنا
+
+521
+00:40:55,420 --> 00:40:59,560
+بنفترض أن الـ antibody is highly specific صح عليها
+
+522
+00:40:59,560 --> 00:41:03,480
+علامة استفهام عليها علامة استفهام لكن لما يكونوا
+
+523
+00:41:03,480 --> 00:41:10,150
+اثنين ومش highly specific أو ثلاثة ومش highly
+
+524
+00:41:10,150 --> 00:41:15,730
+specific فرصة ما يسمى بالـ cross reaction أنا رايح
+
+525
+00:41:15,730 --> 00:41:20,670
+ارتبط مع X ليش ارتبط مع Y؟ إذا ارتبط مع X ومع Y
+
+526
+00:41:20,670 --> 00:41:26,470
+بنسميها cross reaction تفاعل مش طبيعي، يعني تفاعل
+
+527
+00:41:26,470 --> 00:41:30,620
+مع اثنين، أقول تفاعل مع مين؟ مع واحد طيب إذا كان هو
+
+528
+00:41:30,620 --> 00:41:34,660
+antibody واحد، mono فرصة إنه يعمل cross reaction
+
+529
+00:41:34,660 --> 00:41:39,180
+بتكون أقل من لو كانوا اثنين، همام؟ وبالتالي الـ
+
+530
+00:41:39,180 --> 00:41:41,880
+mono-clonal antibody أحدث ثورة في الـ diagnostics،
+
+531
+00:41:41,880 --> 00:41:45,040
+الآن معظم الفحوصات الـ immunology أو الـ serology
+
+532
+00:41:45,040 --> 00:41:48,400
+معتمدة علينا نتكلم عن مين هنا؟ عن mono-clonal
+
+533
+00:41:48,400 --> 00:41:54,460
+antibody لكن لو بنعمل علاج زي اللي قلنا عنه muscle
+
+534
+00:41:54,460 --> 00:41:58,380
+جاب شوية، بتفضلوا mono-clonal ولا polyclonal؟
+
+535
+00:41:59,560 --> 00:42:03,200
+Polyclonal أنه يعمل على كل الـ Epitopes الموجودة
+
+536
+00:42:03,200 --> 00:42:05,560
+على مين على الأنتيجين، يعني أنا مش قصدي أنه .. أنا
+
+537
+00:42:05,560 --> 00:42:09,860
+ما بدي إياكم تأخذوا انطباع أن الـ monoclonal هو الـ top و
+
+538
+00:42:09,860 --> 00:42:12,720
+الـ polyclonal هو إيه، لا ممكن نجي له برضه بعض
+
+539
+00:42:12,720 --> 00:42:17,500
+الاستخدام طبعا الـ antibody اللي بينتج لا يتفاعل
+
+540
+00:42:17,500 --> 00:42:22,680
+المفروض لا يتفاعل إلا مع الـ antigen اللي أحدث اللي
+
+541
+00:42:22,680 --> 00:42:27,050
+هو أنتجوه هذا اللي احنا سميناها specificity، لكن
+
+542
+00:42:27,050 --> 00:42:30,010
+للأسف مش hundred percent specificity، وشفتوا لو
+
+543
+00:42:30,010 --> 00:42:33,590
+بشوية أنتو انه الـ .. الـ .. الـ .. البياض المناعي
+
+544
+00:42:33,590 --> 00:42:37,670
+هاجم بالانتباه السبع للبكتيريا، وهاجم من كمان
+
+545
+00:42:37,670 --> 00:42:41,410
+عضلات القلب، يبقى only specificity أو وين الـ
+
+546
+00:42:41,410 --> 00:42:45,850
+absolute specificity مش موجودة، الـ absolute، احنا
+
+547
+00:42:45,850 --> 00:42:48,450
+بنقول في نوع من الـ specificity، لكن مش absolute
+
+548
+00:42:50,080 --> 00:42:54,600
+طيب، هذه الجملة اللوحة ذكرناها الصحيحة، لكن لا
+
+549
+00:42:54,600 --> 00:42:56,920
+يبقى أسئلة أذكركم فيها إن الـ immunity للـ
+
+550
+00:42:56,920 --> 00:43:00,420
+extracellular bacterial infection والـ immunity to
+
+551
+00:43:00,420 --> 00:43:06,460
+intracellular mainly مين الأولانية humoral لها الـ
+
+552
+00:43:06,460 --> 00:43:10,060
+antibodies بينما الثانية الـ mainly مين الـ cell
+
+553
+00:43:10,060 --> 00:43:14,300
+mediated أو الـ cellular immune اسمينها cellular
+
+554
+00:43:14,300 --> 00:43:16,860
+immunity أو cell mediated immune
+
+555
+00:43:27,870 --> 00:43:35,390
+إيش يعني inadequate؟ غير كافي، غير كافي Causes
+
+556
+00:43:35,390 --> 00:43:40,790
+immune suppression An example is an infection with
+
+557
+00:43:40,790 --> 00:43:45,270
+HIV, which alters T cell immunity and allows
+
+558
+00:43:45,270 --> 00:43:49,320
+further infection with opportunistic pathogens يبقى
+
+559
+00:43:49,320 --> 00:43:54,060
+أحد .. أحد مظاهر اللي قلناها أن الـ immune system
+
+560
+00:43:54,060 --> 00:43:57,580
+بيكون inadequate لما يصاب الإنسان بفيروس الإيدز
+
+561
+00:43:57,580 --> 00:44:02,460
+ففيروس الإيدز بيشتغل على مين هنا؟ على الـ CD4 على
+
+562
+00:44:02,460 --> 00:44:05,800
+الـ CD4 اللي هي الـ T helper cells اللي هي عبارة عن
+
+563
+00:44:05,800 --> 00:44:11,480
+T cells ولو تكون الـ CD4 الـ CD4 cells يعني هي اللي
+
+564
+00:44:11,480 --> 00:44:16,100
+بتعمل orchestrating للـ immune system فبالتالي
+
+565
+00:44:18,010 --> 00:44:23,090
+ما فيش الآن orchestrated immune response يبقى الـ
+
+566
+00:44:23,090 --> 00:44:27,190
+opportunistic pathogens will cause infection لأنه
+
+567
+00:44:27,190 --> 00:44:29,150
+مرض الإيدز بتفكر أن أنت بتموت من الـ .. من الـ
+
+568
+00:44:29,150 --> 00:44:33,310
+virus سبب الإيدز؟ لا هو بيموت من إصابة الأمراض
+
+569
+00:44:33,310 --> 00:44:36,490
+اللي هو خلاص أنت يازر عنده انخفض تماما وهو بتالس
+
+570
+00:44:36,490 --> 00:44:41,120
+أصبح عرضة للإصابة ويمكن كأني قلت لكم أن مرض الإيدز
+
+571
+00:44:41,120 --> 00:44:45,980
+زاد علينا علم الميكروبيولوجي كم كبير وزاد علم ..
+
+572
+00:44:45,980 --> 00:44:51,340
+فهمنا علم المناعة بشكل كبير لأن كثير من
+
+573
+00:44:51,340 --> 00:44:53,840
+الميكروبات اللي كنا بنقول عنها non pathogen ثابت
+
+574
+00:44:53,840 --> 00:44:57,560
+أنها بتعمل infection في مريض مريض الإيدز فصلنا
+
+575
+00:44:57,560 --> 00:44:59,760
+اللي كنا بنقول عنه non pathogen اللي هو
+
+576
+00:44:59,760 --> 00:45:06,290
+opportunistic pathogen فهذه نقطة كتير مهمة طيب،
+
+577
+00:45:06,290 --> 00:45:09,530
+inadequate immune response في عندنا release of
+
+578
+00:45:09,530 --> 00:45:12,610
+toxins that function as super antigens، فاكرين الـ
+
+579
+00:45:12,610 --> 00:45:16,490
+super antigen؟ الصورة هذه، حلوة الصورة اللي هناك،
+
+580
+00:45:16,490 --> 00:45:21,130
+فاهم؟ اللي فوق على الزاوية، فاهم شايفينها؟ هذا
+
+581
+00:45:21,130 --> 00:45:24,470
+عبارة عن super antigen، الـ super antigen، فاكرين
+
+582
+00:45:24,470 --> 00:45:27,070
+ما عشان بدنا نرسم كده الرسم أخيرا؟
+
+583
+00:45:33,380 --> 00:45:36,820
+لما كنا نتكلم عن الـ macrophage ونتكلم عن الـ
+
+584
+00:45:36,820 --> 00:45:41,740
+antigen presenting cells ومش قولنا إننا بنتحول الـ
+
+585
+00:45:41,740 --> 00:45:46,980
+antigen هذا لـ الـ T-cells الآن يفترض أن الـ T-cell
+
+586
+00:45:46,980 --> 00:45:51,860
+يكون 
+
+587
+00:45:51,860 --> 00:45:56,600
+لها receptor مطابق عشان تقدر تاخد مين؟ الـ antigen
+
+588
+00:45:56,600 --> 00:46:02,720
+هذا normal binding حطرة الـ super antigen بيروح
+
+589
+00:46:02,720 --> 00:46:06,600
+بيخلي خليتين واتّفقوا يان بعض وبيلازقون مع بعض زي
+
+590
+00:46:06,600 --> 00:46:13,100
+حاجة فو بيصيروا كانوا إشراقان مقنعوم، binding، هذا
+
+591
+00:46:13,100 --> 00:46:18,380
+بتبقى هو برضه binding، لكن binding specific؟ لا مش
+
+592
+00:46:18,380 --> 00:46:23,480
+specific، وبالتالي هو أي خليتين إجراب من بعض بيروح
+
+593
+00:46:23,480 --> 00:46:27,110
+بيلازقون ببعض فبيعملهم binding فبيصير لنا non
+
+594
+00:46:27,110 --> 00:46:33,210
+specific activation فاكرين لما قلنا قبل دقائق أن
+
+595
+00:46:33,210 --> 00:46:36,910
+هذا الـ non specific activation بيعمل ما يسمى بالـ
+
+596
+00:46:36,910 --> 00:46:41,050
+immunological storm عاصفة مناعية cytokines خلايا
+
+597
+00:46:41,050 --> 00:46:45,830
+وبالتالي يمكن يصير لنا shock والشوك تؤدي إلى
+
+598
+00:46:45,830 --> 00:46:51,070
+الوفاة فممكن تؤدي إلى الوفاة في أن النقطة الثالثة
+
+599
+00:46:51,070 --> 00:46:54,810
+اللي هي الـ evade the immune defenses by altering
+
+600
+00:46:54,810 --> 00:46:57,230
+their antigenic structure هذا ما حكيناه قبل كده
+
+601
+00:46:57,230 --> 00:47:00,950
+أنه قد يخدع جهاز المناعة يعني المناعة هتكون ممتازة
+
+602
+00:47:00,950 --> 00:47:05,230
+ما في مشكلة فيها لكن لأنه الـ .. الـ .. الميكروب نفسه
+
+603
+00:47:05,230 --> 00:47:07,970
+عنده المقدرة على أنه يعمل الـ evasion هو بتاع يفشل
+
+604
+00:47:07,970 --> 00:47:11,490
+أنه يجيب الـ response الطبيعي تبعه المرة الأخرى أن
+
+605
+00:47:11,490 --> 00:47:15,470
+شاء الله سنكمل باقي الـ chapter ونعمل عملية الـ
+
+606
+00:47:15,470 --> 00:47:19,990
+revision فيارات تكون يعني في تحضير لأسئلة محضرين
+
+607
+00:47:19,990 --> 00:47:23,760
+حاجة للنقاط اللي مش فاهمينها أنتو وفي نفس الوقت
+
+608
+00:47:23,760 --> 00:47:27,940
+يكون في عندنا يعني استعداد أنه نجيب على بعض الأسئلة،
+
+609
+00:47:27,940 --> 00:47:30,300
+يعني لو أنا سألت بعض الأسئلة، أنه نجاوب عليها، يعني
+
+610
+00:47:30,300 --> 00:47:34,920
+دراسة quiz، مش quiz، لكن يعني عشان ما تنحرجوش يعني،
+
+611
+00:47:34,920 --> 00:47:36,740
+نجي ونعرفش نجاوب 
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/IMdvAWrllOc_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/IMdvAWrllOc_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..950a0d2bb1be61dfffb57ce7fd53681dd0d96243
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/IMdvAWrllOc_raw.json
@@ -0,0 +1 @@
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أن أذكركم بمثال دراسناه بشكل واقعي ال .. ال .. ال .. ال بالاي ال attachment بالاي تحت ال نيسيريا جونورية", "tokens": [28820, 23758, 281, 1073, 762, 264, 11992, 1185, 49429, 5551, 16572, 3215, 14739, 5551, 8848, 37983, 24793, 4724, 2304, 12984, 6027, 11778, 23557, 3794, 8315, 3224, 4724, 8592, 28820, 36764, 4587, 3615, 1829, 2423, 4386, 2423, 4386, 2423, 4386, 2423, 20666, 47302, 2423, 19431, 20666, 47302, 6055, 33753, 2423, 8717, 1829, 3794, 13546, 25528, 10874, 11536, 13063, 10632], "avg_logprob": -0.34533899618407427, "compression_ratio": 1.4458598726114649, "no_speech_prob": 0.0, "words": [{"start": 127.81, "end": 128.19, "word": "كل", "probability": 0.84521484375}, {"start": 128.19, "end": 128.77, "word": " هذا", "probability": 0.94384765625}, {"start": 128.77, "end": 129.07, "word": " to", "probability": 0.304931640625}, {"start": 129.07, "end": 129.53, "word": " evade", "probability": 0.933837890625}, {"start": 129.53, "end": 129.71, "word": " the", "probability": 0.75830078125}, 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"temperature": 1.0}, {"id": 9, "seek": 22576, "start": 205.18, "end": 225.76, "text": "النقطة الأخيرة في الpart of Genesis اللي هتكلم عنها اليوم اللي هي ال requirement of iron برضه بتدرجع فيكوا للسابق و أذكركم بإنه لما درسنا ال structure of bacteria ما درسناها لكي نحفظ مجوناته و بس لما اتكلمنا عن ال pigment قلنا أحد وضاعي في ال pigment is iron binding", "tokens": [6027, 1863, 47432, 3660, 16247, 9778, 48923, 8978, 2423, 6971, 295, 20587, 13672, 1829, 8032, 2655, 4117, 19528, 18871, 11296, 45595, 20498, 13672, 1829, 39896, 2423, 11695, 295, 6497, 4724, 43042, 3224, 39894, 3215, 47341, 3615, 8978, 4117, 14407, 24976, 3794, 16758, 4587, 4032, 5551, 8848, 37983, 24793, 4724, 28814, 1863, 3224, 5296, 15042, 11778, 2288, 3794, 8315, 2423, 3877, 295, 11763, 19446, 11778, 2288, 3794, 8315, 11296, 5296, 4117, 1829, 8717, 5016, 5172, 19913, 3714, 7435, 2407, 8315, 47395, 4032, 4724, 3794, 5296, 15042, 1975, 2655, 4117, 19528, 8315, 18871, 2423, 31325, 12174, 1211, 8315, 5551, 24401, 4032, 11242, 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398.18, "word": " ال", "probability": 0.73779296875}, {"start": 398.18, "end": 398.4, "word": " limb", "probability": 0.55810546875}], "temperature": 1.0}, {"id": 18, "seek": 42665, "start": 398.87, "end": 426.65, "text": "من ال myeloid بيطلع عندنا ال erythrocytes و ال platelets و الصحيحة دول اللي بنتبرهم مش احنا من جهاز المناعة لكن بيطلع منهم ال neutrophil و ال macrophages، إذا ال neutrophil و ال macrophages هم من ال myeloid tissue بينما ال lymphoid tissue بيطلع عندنا حاجة اسمها natural killer، natural killer و من اسمها يعني وظيفتها الأبدية to kill cells", "tokens": [27842, 2423, 452, 10590, 327, 4724, 1829, 9566, 1211, 3615, 43242, 8315, 2423, 1189, 88, 392, 340, 1344, 7269, 4032, 2423, 3403, 37220, 4032, 31767, 5016, 1829, 5016, 3660, 11778, 12610, 13672, 1829, 4724, 29399, 26890, 16095, 37893, 1975, 5016, 8315, 9154, 10874, 3224, 31377, 9673, 8315, 27884, 44381, 4724, 1829, 9566, 1211, 3615, 9154, 16095, 2423, 7989, 11741, 388, 4032, 2423, 7912, 11741, 1660, 12399, 11933, 15730, 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"probability": 0.98291015625}, {"start": 411.29, "end": 411.75, "word": " و", "probability": 0.98291015625}, {"start": 411.75, "end": 411.89, "word": " ال", "probability": 0.80419921875}, {"start": 411.89, "end": 412.73, "word": " macrophages", "probability": 0.7996419270833334}, {"start": 412.73, "end": 413.37, "word": " هم", "probability": 0.984375}, {"start": 413.37, "end": 413.65, "word": " من", "probability": 0.9951171875}, {"start": 413.65, "end": 413.85, "word": " ال", "probability": 0.7197265625}, {"start": 413.85, "end": 414.81, "word": " myeloid", "probability": 0.9724934895833334}, {"start": 414.81, "end": 415.19, "word": " tissue", "probability": 0.88818359375}, {"start": 415.19, "end": 416.23, "word": " بينما", "probability": 0.771240234375}, {"start": 416.23, "end": 416.37, "word": " ال", "probability": 0.775390625}, {"start": 416.37, "end": 416.85, "word": " lymphoid", "probability": 0.96875}, {"start": 416.85, "end": 417.35, "word": " tissue", "probability": 0.98486328125}, {"start": 417.35, "end": 418.25, "word": " بيطلع", "probability": 0.9775390625}, {"start": 418.25, "end": 418.67, "word": " عندنا", "probability": 0.84423828125}, {"start": 418.67, "end": 419.01, "word": " حاجة", "probability": 0.9326171875}, {"start": 419.01, "end": 419.17, "word": " اسمها", "probability": 0.93505859375}, {"start": 419.17, "end": 419.55, "word": " natural", "probability": 0.904296875}, {"start": 419.55, "end": 420.33, "word": " killer،", "probability": 0.615234375}, {"start": 420.33, "end": 420.63, "word": " natural", "probability": 0.861328125}, {"start": 420.63, "end": 421.15, "word": " killer", "probability": 0.90087890625}, {"start": 421.15, "end": 421.29, "word": " و", "probability": 0.81884765625}, {"start": 421.29, "end": 421.39, "word": " من", "probability": 0.93310546875}, {"start": 421.39, "end": 422.25, "word": " اسمها", "probability": 0.9847005208333334}, {"start": 422.25, "end": 422.77, "word": " يعني", "probability": 0.878173828125}, {"start": 422.77, "end": 423.51, "word": " وظيفتها", "probability": 0.983984375}, {"start": 423.51, "end": 424.47, "word": " الأبدية", "probability": 0.70709228515625}, {"start": 424.47, "end": 424.93, "word": " to", "probability": 0.95703125}, {"start": 424.93, "end": 425.21, "word": " kill", "probability": 0.94091796875}, {"start": 425.21, "end": 426.65, "word": " cells", "probability": 0.89111328125}], "temperature": 1.0}, {"id": 19, "seek": 44774, "start": 427.78, "end": 447.74, "text": "و بيطلع من عندنا منها نوعين كمان مهمين جدا اسمهم T-cells و B-cells ال T-cells طبعا برضه احنا مصنفينها هنا صنفين فقط اللي هم ال CD8 و CD4 و ال CD8 و CD4 هم عبارة عن markers ايش اني markers يعني molecules", "tokens": [2407, 4724, 1829, 9566, 1211, 3615, 9154, 43242, 8315, 9154, 11296, 8717, 45367, 9957, 9122, 2304, 7649, 3714, 16095, 9957, 10874, 28259, 24525, 2304, 16095, 314, 12, 4164, 82, 4032, 363, 12, 4164, 82, 2423, 314, 12, 4164, 82, 23032, 3555, 3615, 995, 4724, 43042, 3224, 1975, 5016, 8315, 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molecules", "probability": 0.7607421875}], "temperature": 1.0}, {"id": 20, "seek": 47720, "start": 447.98, "end": 477.2, "text": "نعم من خلال المتعارف على الخلايا فاسمه حتى الاختصار اللي هو clusters of differentiation CD معناها cluster of differentiation ففيها مجموعة CD8 ومجموعة CD4 الآن ال CD8 بينبتق عنها خلايا اسمها cytotoxic لحظة cytotoxic T cells ايش وضيفتها هذه ال cytotoxic T cells برضه قتل يبقى فيه natural killer", "tokens": [1863, 25957, 9154, 16490, 1211, 6027, 9673, 2655, 3615, 9640, 5172, 15844, 33962, 15040, 25528, 6156, 32277, 2304, 3224, 11331, 49975, 2423, 47283, 2655, 9381, 9640, 13672, 1829, 31439, 23313, 295, 38902, 6743, 3714, 3615, 8315, 11296, 13630, 295, 38902, 6156, 41185, 11296, 3714, 7435, 2304, 2407, 27884, 6743, 23, 4032, 2304, 7435, 2304, 2407, 27884, 6743, 19, 6024, 48506, 2423, 6743, 23, 4724, 9957, 3555, 2655, 4587, 18871, 11296, 16490, 15040, 25528, 24525, 2304, 11296, 40248, 310, 5230, 299, 5296, 5016, 19913, 3660, 40248, 310, 5230, 299, 314, 5438, 1975, 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"probability": 0.8419921875}, {"start": 452.5, "end": 452.64, "word": " اللي", "probability": 0.832763671875}, {"start": 452.64, "end": 452.8, "word": " هو", "probability": 0.91845703125}, {"start": 452.8, "end": 453.64, "word": " clusters", "probability": 0.75}, {"start": 453.64, "end": 454.06, "word": " of", "probability": 0.421142578125}, {"start": 454.06, "end": 455.28, "word": " differentiation", "probability": 0.763671875}, {"start": 455.28, "end": 455.8, "word": " CD", "probability": 0.611328125}, {"start": 455.8, "end": 456.28, "word": " معناها", "probability": 0.686767578125}, {"start": 456.28, "end": 456.64, "word": " cluster", "probability": 0.8271484375}, {"start": 456.64, "end": 456.98, "word": " of", "probability": 0.97705078125}, {"start": 456.98, "end": 457.9, "word": " differentiation", "probability": 0.80322265625}, {"start": 457.9, "end": 458.76, "word": " ففيها", "probability": 0.720703125}, {"start": 458.76, "end": 459.42, "word": " مجموعة", "probability": 0.8515625}, {"start": 459.42, "end": 460.2, "word": " CD8", "probability": 0.865966796875}, {"start": 460.2, "end": 460.86, "word": " ومجموعة", "probability": 0.8893229166666666}, {"start": 460.86, "end": 461.84, "word": " CD4", "probability": 0.977783203125}, {"start": 461.84, "end": 462.72, "word": " الآن", "probability": 0.5272216796875}, {"start": 462.72, "end": 462.88, "word": " ال", "probability": 0.89208984375}, {"start": 462.88, "end": 463.54, "word": " CD8", "probability": 0.87939453125}, {"start": 463.54, "end": 465.16, "word": " بينبتق", "probability": 0.6025146484375}, {"start": 465.16, "end": 465.72, "word": " عنها", "probability": 0.991455078125}, {"start": 465.72, "end": 466.8, "word": " خلايا", "probability": 0.9383138020833334}, {"start": 466.8, "end": 467.56, "word": " اسمها", "probability": 0.939453125}, {"start": 467.56, "end": 469.12, "word": " cytotoxic", "probability": 0.8994140625}, {"start": 469.12, "end": 469.76, "word": " لحظة", "probability": 0.7724609375}, 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هو الميزان اللى حكينا فيه من بداية المحاضرات ال immunity او ال immunity ال ال violence اللى هو ال type of microorganism و ال number بوراس معناها الجرعة او ال number", "tokens": [6027, 2304, 1829, 11622, 7649, 13672, 7578, 3714, 29245, 23328, 12174, 3215, 10943, 4117, 37037, 15730, 15844, 31767, 5172, 13063, 3660, 34051, 15844, 13672, 2407, 5016, 3660, 31439, 9673, 1829, 11622, 7649, 13672, 7578, 11331, 4117, 1829, 8315, 8978, 3224, 9154, 4724, 28259, 10632, 9673, 5016, 46958, 2288, 9307, 2423, 22701, 1975, 2407, 2423, 22701, 2423, 2423, 6270, 13672, 7578, 31439, 2423, 2010, 295, 49129, 1434, 4032, 2423, 1230, 4724, 13063, 32277, 20449, 8315, 11296, 25724, 2288, 27884, 1975, 2407, 2423, 1230], "avg_logprob": -0.2611069363283824, "compression_ratio": 1.725, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 872.15, "end": 872.75, "word": "الميزان", "probability": 0.891015625}, {"start": 872.75, "end": 872.93, "word": " اللى", "probability": 0.76220703125}, {"start": 872.93, 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"text": "و مش highly specific او تلاتة و مش highly specific فرصة ما يسمى بال cross reaction انا رايح ارتبط مع X ليش ارتبط مع Y؟ اذا ارتبط مع X و مع Y بنسميها cross reaction تفاعل مش طبيعي، يعني تفاعل مع اتنين، اقول تفاعل مع مين؟ مع واحد", "tokens": [2407, 37893, 5405, 2685, 1975, 2407, 6055, 1211, 9307, 3660, 4032, 37893, 5405, 2685, 6156, 2288, 9381, 3660, 19446, 7251, 38251, 7578, 20666, 3278, 5480, 1975, 8315, 12602, 47302, 5016, 1975, 43500, 3555, 9566, 20449, 1783, 32239, 8592, 1975, 43500, 3555, 9566, 20449, 398, 22807, 1975, 15730, 1975, 43500, 3555, 9566, 20449, 1783, 4032, 20449, 398, 44945, 38251, 1829, 11296, 3278, 5480, 6055, 5172, 995, 30241, 37893, 23032, 21292, 3615, 1829, 12399, 37495, 22653, 6055, 5172, 995, 30241, 20449, 1975, 2655, 1863, 9957, 12399, 1975, 39648, 6055, 5172, 995, 30241, 20449, 3714, 9957, 22807, 20449, 36764, 24401], "avg_logprob": -0.16246811178873996, "compression_ratio": 1.8177083333333333, "no_speech_prob": 0.0, "words": [{"start": 2465.47, 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101, "seek": 251838, "start": 2489.82, "end": 2518.38, "text": "طيب إذا كان هو antibody واحد، mono فرصة إنه يعمل cross reaction بتكون أقل من لو كانوا اتنين، همام؟ وبالتالي ال mono-clonal antibody أحدث ثورة في ال diagnostics، الآن معظم الفحوصات ال immunology أو ال serology معتمدة علينا نتكلم عن مين هنا؟ عن mono-clonal antibody لكن لو بنعمل علاج زي اللي قلنا عنه muscle جابه شوية، بتفضلوا mono-clonal ولا polyclonal؟", "tokens": [9566, 1829, 3555, 11933, 15730, 25961, 31439, 34507, 36764, 24401, 12399, 35624, 6156, 2288, 9381, 3660, 36145, 3224, 7251, 25957, 1211, 3278, 5480, 39894, 30544, 5551, 4587, 1211, 9154, 45164, 25961, 14407, 1975, 2655, 1863, 9957, 12399, 8032, 2304, 10943, 22807, 46599, 6027, 2655, 6027, 1829, 2423, 35624, 12, 3474, 21523, 34507, 5551, 24401, 12984, 38637, 13063, 3660, 8978, 2423, 43215, 1167, 12399, 6024, 48506, 20449, 19913, 2304, 27188, 5016, 2407, 9381, 9307, 2423, 13154, 1793, 34051, 2423, 816, 1793, 3714, 34268, 2304, 41891, 11203, 9957, 995, 8717, 2655, 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النقطة التالتة اللي هي ال evade", "tokens": [5172, 21292, 25957, 1211, 16095, 17359, 6156, 3555, 9381, 13546, 5296, 8315, 2107, 2685, 24433, 6156, 37983, 9957, 5296, 15042, 12174, 1211, 8315, 12174, 36150, 11778, 4587, 16373, 4587, 16472, 23758, 2423, 2107, 2685, 24433, 4724, 1829, 25957, 1211, 19446, 7251, 38251, 7578, 20666, 13154, 4383, 7679, 6225, 9381, 5172, 3660, 3714, 8315, 3615, 10632, 40248, 453, 1652, 16490, 15040, 25528, 46599, 6027, 2655, 6027, 1829, 7251, 43020, 7251, 9381, 13546, 5296, 8315, 5588, 4032, 25124, 4117, 6055, 33604, 16254, 2423, 7578, 2423, 38688, 995, 3660, 6156, 2304, 43020, 6055, 33604, 16254, 2423, 7578, 2423, 38688, 995, 3660, 8978, 14739, 28239, 47432, 3660, 16712, 6027, 2655, 3660, 13672, 1829, 39896, 2423, 1073, 762], "avg_logprob": -0.2897477968220125, "compression_ratio": 1.7918367346938775, "no_speech_prob": 0.0, "words": [{"start": 2784.63, "end": 2785.21, "word": "فبيعملهم", "probability": 0.6489990234375}, {"start": 2785.21, "end": 2785.59, 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{"start": 2821.91, "end": 2822.63, "word": " لكن", "probability": 0.83837890625}, {"start": 2822.63, "end": 2823.69, "word": " لإنه", "probability": 0.8287353515625}, {"start": 2823.69, "end": 2824.03, "word": " ال", "probability": 0.67138671875}, {"start": 2824.03, "end": 2824.03, "word": " ..", "probability": 0.40234375}, {"start": 2824.03, "end": 2824.37, "word": " ال", "probability": 0.7998046875}, {"start": 2824.37, "end": 2824.49, "word": " ..", "probability": 0.88916015625}, {"start": 2824.49, "end": 2824.85, "word": " الميكروب", "probability": 0.761328125}, {"start": 2824.85, "end": 2825.23, "word": " نفسه", "probability": 0.99609375}, {"start": 2825.23, "end": 2825.47, "word": " عنده", "probability": 0.97314453125}, {"start": 2825.47, "end": 2825.87, "word": " المقدر", "probability": 0.7957356770833334}, {"start": 2825.87, "end": 2825.99, "word": " على", "probability": 0.580078125}, {"start": 2825.99, "end": 2826.13, "word": " انه", "probability": 0.3992919921875}, {"start": 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{"start": 2835.47, "end": 2835.73, "word": " vision", "probability": 0.94580078125}, {"start": 2835.73, "end": 2836.71, "word": " فيارات", "probability": 0.8375651041666666}, {"start": 2836.71, "end": 2837.83, "word": " تكون", "probability": 0.6654052734375}, {"start": 2837.83, "end": 2838.21, "word": " يعني", "probability": 0.850341796875}, {"start": 2838.21, "end": 2838.37, "word": " في", "probability": 0.9560546875}, {"start": 2838.37, "end": 2838.77, "word": " تحضير", "probability": 0.9798583984375}, {"start": 2838.77, "end": 2839.33, "word": " لأسئلة", "probability": 0.9138671875}, {"start": 2839.33, "end": 2839.99, "word": " محضرين", "probability": 0.767724609375}, {"start": 2839.99, "end": 2840.17, "word": " حاجة", "probability": 0.7692057291666666}, {"start": 2840.17, "end": 2840.81, "word": " للنقاط", "probability": 0.7801513671875}, {"start": 2840.81, "end": 2840.91, "word": " اللي", "probability": 0.934326171875}, {"start": 2840.91, "end": 2841.03, "word": " مش", 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8592, 37495, 22653, 12399, 8717, 7435, 5172, 4032, 15042, 1863, 3615, 28480, 8592, 8717, 7435, 995, 37746], "avg_logprob": -0.23009867981860513, "compression_ratio": 1.8636363636363635, "no_speech_prob": 1.1920928955078125e-06, "words": [{"start": 2842.54, "end": 2843.12, "word": "وفي", "probability": 0.663818359375}, {"start": 2843.12, "end": 2843.28, "word": " نفس", "probability": 0.9970703125}, {"start": 2843.28, "end": 2843.76, "word": " الوقت", "probability": 0.9640299479166666}, {"start": 2843.76, "end": 2844.24, "word": " يكون", "probability": 0.982421875}, {"start": 2844.24, "end": 2844.44, "word": " في", "probability": 0.96240234375}, {"start": 2844.44, "end": 2844.86, "word": " عنا", "probability": 0.645263671875}, {"start": 2844.86, "end": 2845.32, "word": " يعني", "probability": 0.628173828125}, {"start": 2845.32, "end": 2846.54, "word": " استعداد", "probability": 0.9596354166666666}, {"start": 2846.54, "end": 2846.9, "word": " أنه", "probability": 0.6534423828125}, {"start": 2846.9, "end": 2847.2, "word": " نجيب", "probability": 0.9967041015625}, {"start": 2847.2, "end": 2847.34, "word": " على", "probability": 0.72265625}, {"start": 2847.34, "end": 2847.58, "word": " بعض", "probability": 0.994140625}, {"start": 2847.58, "end": 2847.94, "word": " الأسئلة،", "probability": 0.93642578125}, {"start": 2847.94, "end": 2848.0, "word": " يعني", "probability": 0.956787109375}, {"start": 2848.0, "end": 2848.08, "word": " لو", "probability": 0.970703125}, {"start": 2848.08, "end": 2848.22, "word": " أنا", "probability": 0.900390625}, {"start": 2848.22, "end": 2848.42, "word": " سأل", "probability": 0.9412434895833334}, {"start": 2848.42, "end": 2848.66, "word": " بعض", "probability": 0.954345703125}, {"start": 2848.66, "end": 2849.36, "word": " الأسئلة،", "probability": 0.883056640625}, {"start": 2849.36, "end": 2849.62, "word": " أنه", "probability": 0.723876953125}, {"start": 2849.62, "end": 2849.96, "word": " نجاوب", "probability": 0.9818115234375}, 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\ No newline at end of file
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/KvmHhaw5nZI.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/KvmHhaw5nZI.srt
new file mode 100644
index 0000000000000000000000000000000000000000..6d1010dc01b2ccf2591525efc3fe332904cf317b
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/KvmHhaw5nZI.srt
@@ -0,0 +1,2119 @@
+1
+00:00:20,640 --> 00:00:24,800
+بسم الله الرحمن الرحيم استكمالا للموضوع الذي 
+
+2
+00:00:24,800 --> 00:00:26,420
+بدأناه المحاضرة السابقة الذي هو ال bacterial
+
+3
+00:00:26,420 --> 00:00:30,700
+genetics هنمر مرة سريعة على كل النقاط التي مرينا بها
+
+4
+00:00:30,700 --> 00:00:34,280
+المحاضرة السابقة لكن بشكل سريع جدا تذكروا أن
+
+5
+00:00:34,280 --> 00:00:36,720
+المادة التي قرار فيها بالبكتيريا هي واحدة من ثلاثة
+
+6
+00:00:36,720 --> 00:00:40,440
+يا إما كروموزوم يا إما بلازميد يا إما إبيزوم
+
+7
+00:00:40,440 --> 00:00:45,060
+والإبيزوم هو حقا بلازميد يعني يا كروموزوم يا بلازميد
+
+8
+00:00:45,060 --> 00:00:50,270
+في وقتها لما تكلمنا عن ال mutation تحدثنا عن نقطة
+
+9
+00:00:50,270 --> 00:00:55,790
+مهمة جدا كنا أنه يعني تغيير طفيف في تركيب كائن
+
+10
+00:00:55,790 --> 00:00:58,330
+واحد زي الموجود الذي هو حلمان أعطانا خمس
+
+11
+00:00:58,330 --> 00:01:03,550
+varieties خمس أصناف اختلفت في اللون ممكن في الشكل
+
+12
+00:01:03,550 --> 00:01:06,110
+ممكن في المواصفات ممكن في القدرة على ال survival
+
+13
+00:01:06,110 --> 00:01:12,150
+فالمutation أساس مهم جدا في حياة ال micro organism
+
+14
+00:01:12,150 --> 00:01:16,560
+لأنه single chromosome إذا تتذكروا وقلنا only one
+
+15
+00:01:16,560 --> 00:01:20,560
+copy of the gene فإذا صار فيه طفرة فستظهر عليها ال
+
+16
+00:01:20,560 --> 00:01:24,820
+phenotype  سبعتها نحن ال eukaryotes لو صار طفرة
+
+17
+00:01:24,820 --> 00:01:27,200
+على كروموزوم على سبيل المثال على ال gene الثاني
+
+18
+00:01:27,200 --> 00:01:29,980
+لسه مازال موجود وبالتالي يمكن يغطي على تأثير
+
+19
+00:01:29,980 --> 00:01:33,800
+الطفرة الأولاني فظهور الطفرات في البكتيريا بيكون
+
+20
+00:01:33,800 --> 00:01:38,760
+أسرع قلنا عن ال genetic variation في البكتيريا
+
+21
+00:01:38,760 --> 00:01:42,060
+وقلنا إنه ال genetic variation تنشأ عن إما
+
+22
+00:01:42,060 --> 00:01:45,610
+mutation وذكرنا ال mutation كنا فيه منها صنفين
+
+23
+00:01:45,610 --> 00:01:49,890
+spontaneous و induced واتكلمنا عن ال gene
+
+24
+00:01:49,890 --> 00:01:52,130
+transfer وبدأنا في الحديث عن ال transformation
+
+25
+00:01:52,130 --> 00:01:56,030
+وصفناه بأنه ال free take up of DNA أو uptake لل
+
+26
+00:01:56,030 --> 00:01:59,530
+DNA من ال surroundings ال conjugation هو الاقتران
+
+27
+00:01:59,530 --> 00:02:02,550
+الجنسي عبر البلازميد وال transduction درسناه من
+
+28
+00:02:02,550 --> 00:02:06,010
+نوعين الذي هو ال generalized وال specialized
+
+29
+00:02:06,010 --> 00:02:09,750
+حنمر بشوية تفاصيل كلمنا عن مصطلح اسمه lysogenic
+
+30
+00:02:09,750 --> 00:02:12,970
+conversion البكتيريا صار جواها profage
+
+31
+00:02:13,980 --> 00:02:16,640
+البكتيريوفاجي الذي جواها اسمه Profagia البكتيري
+
+32
+00:02:16,640 --> 00:02:20,080
+الذي حاملة ال Profagia اسمها Lysogenized cell أو
+
+33
+00:02:20,080 --> 00:02:24,020
+Lysogenized bacteria واخر وقت تكلمنا عنها في ال
+
+34
+00:02:24,020 --> 00:02:26,760
+gene في ال genetic variation الذي ال Protoplast
+
+35
+00:02:26,760 --> 00:02:32,480
+fusion wherein you unite two cells into one single
+
+36
+00:02:32,480 --> 00:02:36,280
+cell أكيد هيحصل في ال combination من ال genome تبع
+
+37
+00:02:36,280 --> 00:02:41,700
+الخلية الأولى وال genome تبع الخلية الثانية وبدأنا
+
+38
+00:02:41,700 --> 00:02:44,440
+بالحوار أو بحوارنا المرة الماضية عن ال mutation
+
+39
+00:02:44,440 --> 00:02:48,140
+وكنا برضه هي صنفان نوعا ما التي هي ال base
+
+40
+00:02:48,140 --> 00:02:50,820
+substitution وال frame shift وال frame shift
+
+41
+00:02:50,820 --> 00:02:55,740
+نوعا ما إما insertion أو deletion لكن في المحصلة
+
+42
+00:02:55,740 --> 00:02:59,580
+النهائية ال mutation هي التغيير في ال sequence إيش
+
+43
+00:02:59,580 --> 00:03:04,140
+هي ال sequence؟ التعبر أو ال structure تبع الذي هو
+
+44
+00:03:04,140 --> 00:03:05,740
+المادة الوراثية
+
+45
+00:03:08,000 --> 00:03:11,200
+base substitution أو frame shift وللـ frame shift
+
+46
+00:03:11,200 --> 00:03:15,160
+قلنا insertion أو deletion اللوحة التي ما تكلمنا
+
+47
+00:03:15,160 --> 00:03:21,500
+عنها التي هي ال mutagenes ال مواد المطفرة المنتجة
+
+48
+00:03:21,500 --> 00:03:25,960
+للطفرات طبعا
+
+49
+00:03:25,960 --> 00:03:29,340
+اللوحة التي قدامكم هي نموذج مثال بسيط مثال بسيط
+
+50
+00:03:29,340 --> 00:03:36,540
+على مركبات أو مواد قد تسبب طفرة وسميناها ميوتاجين
+
+51
+00:03:37,480 --> 00:03:40,480
+chemicals زي نيتروز أسيد وهو يتبع لمجموعة اسمها
+
+52
+00:03:40,480 --> 00:03:44,680
+alkalating agent حنمر ذكرها معنا لاحقا five
+
+53
+00:03:44,680 --> 00:03:49,080
+bromioracil five bromioracil هو structural analog
+
+54
+00:03:49,080 --> 00:03:52,720
+وبدأ أشرح إيش يعني structural analog لأنه هذا
+
+55
+00:03:52,720 --> 00:03:57,060
+المفهوم كثير مهم بالنسبة لنا في مجالات متنوعة في
+
+56
+00:03:57,060 --> 00:04:02,600
+ال micro biome لو عندي أنا توأم طفلين توأم تمام
+
+57
+00:04:02,600 --> 00:04:08,740
+identical أنا مش قادر أميز بينهم واحد منهم بنعطيه
+
+58
+00:04:08,740 --> 00:04:15,660
+مبلغ معين من المال، تمام؟ فبيصرفوه على المنزل،
+
+59
+00:04:15,660 --> 00:04:20,680
+بيدبر فيه، بيشتري فيه أكل، ملابس، إلى آخره، الثاني
+
+60
+00:04:20,680 --> 00:04:23,880
+لو أعطينا نفس المبلغ ليش بيعمل فيه، بيحطه و يضبه
+
+61
+00:04:23,880 --> 00:04:29,230
+في جيبه، ما حدش بيستفيد منه الـ original الطفل
+
+62
+00:04:29,230 --> 00:04:32,930
+الأول هو الإنزايم على سبيل المثال أو المركب
+
+63
+00:04:32,930 --> 00:04:36,470
+المفيد في الخلية، الـ analog له الذي هو الشبيه
+
+64
+00:04:36,470 --> 00:04:40,490
+تبعه، الذي غير مفيد، ف structural analog،
+
+65
+00:04:40,490 --> 00:04:46,650
+structural analog، الآن هذا المفهوم تخيله كالتالي،
+
+66
+00:04:46,650 --> 00:04:50,600
+عندي إنزيم بيشتغل على substrate، لما .. لما ال
+
+67
+00:04:50,600 --> 00:04:54,020
+substrate الحقيقي بيكون موجود بيكسره ل products،
+
+68
+00:04:54,020 --> 00:04:57,060
+ال products هذه بتكسر ل products أخرى وبتفيد
+
+69
+00:04:57,060 --> 00:05:00,740
+الخلية، لكن لو ال substrate الثاني الذي هو ال
+
+70
+00:05:00,740 --> 00:05:02,160
+analog ارتبط بالإنزيم
+
+71
+00:05:14,920 --> 00:05:19,200
+فقد نقتل الخلية السرطانية بنفس الطريقة بنحط
+
+72
+00:05:19,200 --> 00:05:23,340
+structural analog قد نقتل البكتيريا بنحط
+
+73
+00:05:23,340 --> 00:05:30,580
+structural analog قد نقتل نحن ب structural analog
+
+74
+00:05:32,280 --> 00:05:35,160
+الـ Phi-Promo-Uracil Structure Analog لـ Natural
+
+75
+00:05:35,160 --> 00:05:38,520
+Genes Basis في الـ في الـ DNA فالـ DNA Polymerase
+
+76
+00:05:38,520 --> 00:05:42,380
+بدل ما يحط Base معينة بيحط مين بدلها الـ Phi-Promo
+
+77
+00:05:42,380 --> 00:05:47,100
+-Uracil الـ Phi-Promo-Uracil مش Base حقيقية Analog
+
+78
+00:05:47,100 --> 00:05:51,680
+فلما بيجي بيكمل الـ DNA بقدرش يربط القاعدة الأخرى
+
+79
+00:05:51,680 --> 00:05:56,060
+وراها يبقى وجدنا تصنيع الـ DNA لما صار عندي
+
+80
+00:05:56,060 --> 00:05:57,720
+mutation لما ماصارش عندي mutation صار عندي
+
+81
+00:05:57,720 --> 00:05:58,240
+mutation
+
+82
+00:06:03,080 --> 00:06:07,800
+لكن عندي chemicals لو أنا بدأت أقعد أعمل قائمة فيها
+
+83
+00:06:07,800 --> 00:06:11,960
+يمكن أقعد ساعة كاملة أعمل listing فقط لأسماء
+
+84
+00:06:11,960 --> 00:06:16,840
+المركبات التي ممكن تعمل ال mutation تسمعوا في
+
+85
+00:06:16,840 --> 00:06:20,300
+المستشفيات أن الست الحامل هنا تدخل على غرف الأشعة
+
+86
+00:06:20,300 --> 00:06:25,320
+وأن الذي بيصوره في الأشعة بيلبس سترة واقية مكونة من
+
+87
+00:06:25,320 --> 00:06:31,640
+الرصاص ويدري أن الغرف بتكون مبطنة بالرصاص لماذا؟
+
+88
+00:06:31,640 --> 00:06:35,920
+لأن الـ X-ray can cause mutation ممنوع تتعرض له
+
+89
+00:06:35,920 --> 00:06:40,400
+الـ UV في المختبر، في الميكروسكوبات أو في اللمبات
+
+90
+00:06:40,400 --> 00:06:46,910
+تبع التعقيم، بنحط حادث shield plastic أو شيء معتم
+
+91
+00:06:46,910 --> 00:06:51,370
+و بيقول ممنوع أن ال UV تصيب العين أو تجرب على
+
+92
+00:06:51,370 --> 00:06:54,370
+الجلد لأنه بتعمل mutation هتكلم لحظة نحن كيف تعمل
+
+93
+00:06:54,370 --> 00:06:58,610
+ال mutation لكن هاي x-ray أو ionizing و non
+
+94
+00:06:58,610 --> 00:07:01,710
+-ionizing radiation تمام عملوا mutation و
+
+95
+00:07:01,710 --> 00:07:05,850
+chemicals عملوا mutation النقطة الأخيرة ال viruses
+
+96
+00:07:05,850 --> 00:07:10,790
+هناك مجموعة من الفيروسات تسمى onco viruses onco
+
+97
+00:07:10,790 --> 00:07:16,090
+viruses وهي فيروسات تسبب mutation في الإنسان وحتى
+
+98
+00:07:16,090 --> 00:07:20,650
+في الحيوان طبعا يعني أنا كلمة مرة الإنسان هذه مقدمة
+
+99
+00:07:20,650 --> 00:07:24,830
+لنا نحن نعرف أنه فيه مركبات أو عوامل بتسبب
+
+100
+00:07:24,830 --> 00:07:28,830
+المتايشير لكن هذا .. هذه ليست قائمة حصرية وإنما
+
+101
+00:07:28,830 --> 00:07:34,330
+sample okay لسه لسه احكي شغل بطبع نسبة الأشياء
+
+102
+00:07:34,330 --> 00:07:38,830
+عامة في الشركة التي بتتحدث عنها مابتدبسوش واقعي و
+
+103
+00:07:38,830 --> 00:07:42,270
+حتى أنا ماريا اتفتت مع شخص على هذا وعادي يعني
+
+104
+00:07:42,270 --> 00:07:48,140
+سوروه وتمي أشياء ال precautions يعني موجود، يعني
+
+105
+00:07:48,140 --> 00:07:53,240
+في .. في ال .. الجدار بيختفي وراه الذي هو المصور
+
+106
+00:07:53,240 --> 00:07:57,660
+الأشعة، هذا الجدار باقي، لكن .. يعني خدنا نقول
+
+107
+00:07:57,660 --> 00:08:01,260
+مابيخدوش الاحتياطات الدورية مائة بالمائة، لكن يصرف
+
+108
+00:08:01,260 --> 00:08:04,680
+لهم واجبات خصوصي موظفي الأشعة في المستشفيات أنا في
+
+109
+00:08:04,680 --> 00:08:06,840
+فلسطين، يصرف .. يصرف لهم
+
+110
+00:08:11,310 --> 00:08:14,990
+لكن ربما يكون إهمال من البعض أنه ما بيرتزم أما
+
+111
+00:08:14,990 --> 00:08:19,690
+موجود الكلام لما تكلمنا عن ال mutation تكلمنا عن
+
+112
+00:08:19,690 --> 00:08:23,350
+حزمة spontaneous mutation وعزوناها إلى mistakes
+
+113
+00:08:23,350 --> 00:08:27,650
+في ال enzymes خلال عملية الذي هو ال replication أو
+
+114
+00:08:27,650 --> 00:08:31,370
+ال transcription وكنا أن ال mutation rate كانت
+
+115
+00:08:31,370 --> 00:08:35,850
+واحد في عشرة قوس ثمانية كل مائة مليون في إمكانية
+
+116
+00:08:35,850 --> 00:08:40,430
+لطفرة واحدة لكن اتفقنا أن هذا رقم ضخم، رقم ضخم
+
+117
+00:08:40,430 --> 00:08:44,670
+بالمقارنة مع مين؟ مع الانقسامات العديدة التي للبكتيريا
+
+118
+00:08:45,690 --> 00:08:48,810
+mutation and selectivity مفهوم برضه لازم نوضحه
+
+119
+00:08:48,810 --> 00:08:53,770
+الآن هذا mutation and selectivity تخيلوا أمامنا
+
+120
+00:08:53,770 --> 00:08:56,790
+على الطاولة هذه على سطح هذا الذي قدامنا في عندنا
+
+121
+00:08:56,790 --> 00:09:01,250
+مليار بكتيريا كلهم حساسين ل antibiotic اسمه
+
+122
+00:09:01,250 --> 00:09:06,510
+penicillin تمام؟ ما عدا بكتيريا واحدة استطاعت أن
+
+123
+00:09:06,510 --> 00:09:10,910
+تكون resistance بطريقة ما كل ما لها فلو
+
+124
+00:09:10,910 --> 00:09:14,310
+وضعنا نحن الآن البنسلين على الطاولة كل المليار
+
+125
+00:09:14,310 --> 00:09:17,690
+هيموت ما عدا هذه البكتيريا اخترناها ولا ما اخترناها
+
+126
+00:09:17,690 --> 00:09:22,990
+احنا الآن؟ كيف اخترناها؟ بأنه أضفنا المادة القاتلة
+
+127
+00:09:22,990 --> 00:09:26,910
+للأخرى يعني لا تلاحظوا شغل ثاني على الاختيار هذه
+
+128
+00:09:26,910 --> 00:09:31,350
+البكتيريا هذه كانت واحدة من المليار تخيلوا أنتم في
+
+129
+00:09:31,350 --> 00:09:36,110
+الفصل اللي قدامنا الآن خمسين طالبة فرصتكم كنتم 
+
+130
+00:09:36,110 --> 00:09:40,950
+تسألوني سؤال كل واحدة واحد اثنين في المئة يعني 2%
+
+131
+00:09:40,950 --> 00:09:46,890
+الآن لو لم تبقَ لطالبة واحدة الفصل ففرصة نتساءل 
+
+132
+00:09:46,890 --> 00:09:52,310
+السؤال 100% فالآن أخذت فرص الآخرين فالبكتيريا
+
+133
+00:09:52,310 --> 00:09:56,370
+الواحدة اللي موجودة برضه أخذت فرص الآخرين من أي
+
+134
+00:09:56,370 --> 00:10:00,910
+ناحية من ناحية البيئة الموجودة الغذاء فتكاثرَت بدون
+
+135
+00:10:00,910 --> 00:10:05,330
+ما يكون في لها أي منافسين يبقى اخترناها ولا ما
+
+136
+00:10:05,330 --> 00:10:10,440
+اخترناهاش selectivity طيب لو قلنا لكم أن مقاومتها 
+
+137
+00:10:10,440 --> 00:10:16,520
+للـ بنسلين على سبيل المثال نشأت عن طفرة فمن سبب
+
+138
+00:10:16,520 --> 00:10:21,740
+الاختيار؟ طفرة يعني بمعنى الحكي أن الطفرات قد
+
+139
+00:10:21,740 --> 00:10:26,960
+تنتخب، قد تختار، قد تميز أنواع معينة من البكتيريا 
+
+140
+00:10:26,960 --> 00:10:30,460
+وتنجحها على الأنواع الأخرى أو أعطيكم مثال أنا بسيط
+
+141
+00:10:30,460 --> 00:10:36,920
+جدا، لو جبنا بكتيريا وحطيناها في صحراء، سحراء، مش
+
+142
+00:10:36,920 --> 00:10:42,620
+أكل اهو ولا شرب، هتموت من الجوع، نفترض واحدة من
+
+143
+00:10:42,620 --> 00:10:44,540
+البكتيريا الموجودة اللي في اللي حاطناه من هناك
+
+144
+00:10:44,540 --> 00:10:50,460
+الأرواح استطاعت أن .. هذا مثال readily، بس مجرد
+
+145
+00:10:50,460 --> 00:10:54,220
+التوضيح، أن تتغذى على الرمل، أن تستخدم الرمل
+
+146
+00:10:54,220 --> 00:10:57,900
+كمصدر كربوني، كمصدر كربوني، بيطفر
+
+147
+00:11:06,320 --> 00:11:10,800
+لا وهذا يحدث حقيقة في البيئات بشكل مستمر لذلك أنا
+
+148
+00:11:10,800 --> 00:11:14,000
+لا أنا دائما بقول أن ما نسميه احنا spontaneous
+
+149
+00:11:14,000 --> 00:11:17,700
+mutation قد لا يكون spontaneous وقد يكون مبرمج أن
+
+150
+00:11:17,700 --> 00:11:21,560
+البكتيريا يصير فيها طفرات حتى تتأقلم مع الظروف
+
+151
+00:11:21,560 --> 00:11:25,800
+المتغيرة في هذا العالم طيب ليه مصطلح ثاني اسمه
+
+152
+00:11:25,800 --> 00:11:30,980
+backward mutation تخيلوا البكتيريا هذه اللي صار
+
+153
+00:11:30,980 --> 00:11:36,720
+فيها mutation وصارت بصفة معينة أو بدون صفة، فقدت
+
+154
+00:11:36,720 --> 00:11:40,820
+صفة، طبعا؟ صار فيها ثاني يوم mutation في نفس ال
+
+155
+00:11:40,820 --> 00:11:45,360
+gene رجع ال mutation الأولانية إلى ال original،
+
+156
+00:11:45,360 --> 00:11:48,080
+إيش بدنا نسميها الآن؟ back mutation
+
+157
+00:11:55,550 --> 00:11:59,410
+وبالتالي رجعت البكتيريا للـ wild type أو لل
+
+158
+00:11:59,410 --> 00:12:02,170
+original genotype
+
+159
+00:12:07,720 --> 00:12:10,520
+الـ transformation ما .. ما أظن أنه لا ومحتاج
+
+160
+00:12:10,520 --> 00:12:13,900
+أتكلم عنها كثيرا لكن في اللوحة اللي أمامكم في
+
+161
+00:12:13,900 --> 00:12:18,280
+الفقرة الثانية في أمثلة على أسماء بكتيريا معروفة
+
+162
+00:12:18,280 --> 00:12:23,180
+أنه قادرة عليها تأخذ DNA من الـ surrounding واش
+
+163
+00:12:23,180 --> 00:12:27,220
+تملؤ .. تملؤ incorporation أو recombination يعني
+
+164
+00:12:27,220 --> 00:12:30,900
+اللي هو دمجوا في المادة الوراثية تبعها زي الـ bacillus
+
+165
+00:12:30,900 --> 00:12:35,440
+و الـ hemophilus و الـ Neisseria و الـ pneumococcus هو اسم
+
+166
+00:12:35,440 --> 00:12:37,020
+آخر لـ streptococcus pneumonia
+
+167
+00:12:40,700 --> 00:12:44,720
+هذه الصورة ترينا المادة الوراثية اللي هي الـ free
+
+168
+00:12:44,720 --> 00:12:45,460
+DNA
+
+169
+00:12:48,910 --> 00:12:52,770
+وبعدين بتعملها incorporation داخل الـ DNA فال
+
+170
+00:12:52,770 --> 00:12:57,710
+answer في أن دي إن إيه من جسم غريب أو من خلية غريبة أو
+
+171
+00:12:57,710 --> 00:13:00,750
+من خلية متبرعة إلى داخل الخلية اللي يسميناها
+
+172
+00:13:00,750 --> 00:13:05,610
+recipient cell طبعا إذا بتقارنه هاي الـ original و
+
+173
+00:13:05,610 --> 00:13:08,510
+الـ original ده بيسميه البكتيريا الـ wild
+
+174
+00:13:12,260 --> 00:13:18,180
+و الثانية اللي سموها transformant اللي تم نقل مادة
+
+175
+00:13:18,180 --> 00:13:23,900
+وراثية جديدة مهمة المصطلحات هذه أن تتعرف علي ال
+
+176
+00:13:23,900 --> 00:13:27,770
+wild type و الـ transformant الـ conjugation حكينا عن
+
+177
+00:13:27,770 --> 00:13:31,490
+نوع واحد من الـ .. أو نوعين من الـ conjugation ده
+
+178
+00:13:31,490 --> 00:13:34,790
+بتذكروا حكينا عن اللي هو الـ F positive و الـ F
+
+179
+00:13:34,790 --> 00:13:38,350
+negative و حكينا عن الـ HFR الـ HFR هو الـ high
+
+180
+00:13:38,350 --> 00:13:41,690
+frequency الـ combination اللي هو متعلق بالإيبزوم
+
+181
+00:13:41,690 --> 00:13:45,450
+فكان لما كانت البلازميد مرتبطة بالكروموسوم
+
+182
+00:13:52,530 --> 00:13:55,770
+الـ transduction طبعا العنوان الأساسي كان تبعه ال
+
+183
+00:13:55,770 --> 00:14:00,310
+bacteriophage و الـ bacteriophage اللي أمامكم يعني
+
+184
+00:14:00,310 --> 00:14:05,450
+التركيب تبعته واضحة أنه فيه له head رأس وفيه له 
+
+185
+00:14:05,450 --> 00:14:08,570
+اللي هو الـ collar أو العنق هذا وفيه له الـ tail
+
+186
+00:14:08,570 --> 00:14:13,750
+fiber طبعا هنشوف تركيب أكثر التسمية bacteriophage
+
+187
+00:14:13,750 --> 00:14:18,050
+تعطيكم إيحاء بإيش؟ بإنه bacteriophage
+
+188
+00:14:19,850 --> 00:14:23,350
+آكلة آكلة وهي تسمية خاطئة الفيروس أصغر من
+
+189
+00:14:23,350 --> 00:14:28,150
+البكتيريا لكن كونه تسمية شائعة فبالتالي لا يبدأ على
+
+190
+00:14:28,150 --> 00:14:30,610
+تغيير الاسم خلاص ده اسمه bacteriophage بندله
+
+191
+00:14:30,610 --> 00:14:35,570
+اختصارا فاج لكن كنتم تعرفوا أن الـ bacteriophage
+
+192
+00:14:35,570 --> 00:14:39,110
+بيقولنا في حاجة اسمها specificity وسبب ال
+
+193
+00:14:39,110 --> 00:14:42,970
+specificity نجم عن مين؟ على الـ tail fibers اللي
+
+194
+00:14:42,970 --> 00:14:45,610
+بيقولهم الـ receptor على الـ cell wall تبقى البكتيريا
+
+195
+00:14:47,390 --> 00:14:52,090
+باختصار what are viruses؟ viruses are viruses that
+
+196
+00:14:52,090 --> 00:14:56,570
+infect bacteria، لا ننسى أنهم viruses، لكن هذه
+
+197
+00:14:56,570 --> 00:14:59,990
+الفيروس مقصوصة فقط للبكتيريا، يعني they do not
+
+198
+00:14:59,990 --> 00:15:04,850
+infect human or animals or plants عشان لما أقول لكم
+
+199
+00:15:04,850 --> 00:15:08,930
+أنه ممكن نستخدم الـ bacterium في العلاج، ما تقولوش
+
+200
+00:15:08,930 --> 00:15:12,390
+طب ما هي بتضر الإنسان، لا بتضر الإنسان على طول
+
+201
+00:15:12,390 --> 00:15:14,910
+الإنسان لو دخلت .. لو دخلت الدم بتعرف عليهم على
+
+202
+00:15:14,910 --> 00:15:17,690
+أنهم أجسام غريبة بيأكلهم بكل سهولة لأنهم ما
+
+203
+00:15:17,690 --> 00:15:22,750
+بيقدروا يعملوا infection للإنسان طب كيف تلقوا ..
+
+204
+00:15:22,750 --> 00:15:27,150
+بعملوا .. سؤال .. سؤال وإجابة تذكروا أنه مش دائما
+
+205
+00:15:27,150 --> 00:15:31,530
+احنا بنصل جهاز المناعة، يعني مثلا ممكن على skin
+
+206
+00:15:33,080 --> 00:15:37,140
+ما فيش جهاز منها للالتهابات ممكن نعمل local على ال
+
+207
+00:15:37,140 --> 00:15:40,940
+tonsils من جوا نعمل spray على الـ tonsils ممكن نحط
+
+208
+00:15:40,940 --> 00:15:45,220
+البكتيريا oral فتشربها ناكلها وتنزل في الأمعاء
+
+209
+00:15:45,220 --> 00:15:48,260
+تقطر البكتيريا اللي فيها بعيدا عن الـ phagocytes و
+
+210
+00:15:48,260 --> 00:15:53,340
+عن الـ macrophages خلينا
+
+211
+00:15:53,340 --> 00:16:01,020
+ناخد الشكل هذا للأسف هذا يبدو لو فيه مشكلة
+
+212
+00:16:01,020 --> 00:16:01,700
+في عرض الـ ..
+
+213
+00:16:05,480 --> 00:16:08,400
+شايفين اللوحة اللي قدامكم هذه تركيب بسيط جدا
+
+214
+00:16:08,400 --> 00:16:14,500
+للـ bacteriophage طالبا على الشكل اللي أنا الآن بضغط
+
+215
+00:16:14,500 --> 00:16:19,580
+عليه هذا الشكل هو اللي بسموه capsid و الـ capsid
+
+216
+00:16:19,580 --> 00:16:24,440
+عبارة عن protein envelope مكون من عشرين متلّات
+
+217
+00:16:24,440 --> 00:16:30,180
+بسموه icosahedral اكتبها
+
+218
+00:16:46,480 --> 00:16:59,460
+ايكوساهيدرال ايكوساهيدرال ايكوساهيدرال
+
+219
+00:16:59,460 --> 00:17:04,080
+ايكوساهيدرال ايكوساهيدرال ايكوساهيدرال ايكوساهيدرال
+
+220
+00:17:04,080 --> 00:17:06,320
+ايكوساهيدرال ايكوساهيدرال ايكوساهيدرال ايكوساهيدرال
+
+221
+00:17:06,320 --> 00:17:06,500
+ايكوساهيدرال ايكوساهيدرال ايكوساهيدرال ايكوساهيدرال
+
+222
+00:17:06,500 --> 00:17:10,290
+ايكوساهيدرال الصحيفي عندي في الـ .. في الـ .. الوزر
+
+223
+00:17:10,290 --> 00:17:13,650
+الصفلي تركيبين مهمين جدا اللي هم الـ tail fibers و
+
+224
+00:17:13,650 --> 00:17:16,170
+الـ tail fibers كمان مرة هم المسؤولين عن مين
+
+225
+00:17:19,160 --> 00:17:23,260
+اللي هو الالتصاق مع الـ receptors وفي قاعدة base
+
+226
+00:17:23,260 --> 00:17:29,580
+plate الـ base plate عبارة عن hooks زي خطافات تلتصق
+
+227
+00:17:29,580 --> 00:17:33,260
+بالجدار وهذا الجدار أو هذا الجزء بيحتوي على
+
+228
+00:17:33,260 --> 00:17:38,660
+إنزيمات هاضمة إنزيمات هاضمة لجدار البكتيريا عشان
+
+229
+00:17:38,660 --> 00:17:42,060
+يصير يعمل فيه إيه injection للمادة الوراثية 
+
+230
+00:17:48,590 --> 00:17:53,050
+في حياتنا المحاضرة السابقة، تعرضنا لدراسة من ال
+
+231
+00:17:53,050 --> 00:17:57,410
+bacterium فعشان، الـ lytic و الـ lysogenic، ال
+
+232
+00:17:57,410 --> 00:18:00,670
+lytic اللي دخل على الخلية وقال لهم أنتم الآن تحت
+
+233
+00:18:00,670 --> 00:18:04,090
+سيطرتي، وهدّر الخلية ودمرها وانتهى، هذا اسمه
+
+234
+00:18:04,090 --> 00:18:08,550
+lytic أو virulent فعشان، virulent يعني شرس
+
+235
+00:18:14,940 --> 00:18:18,360
+وطبعا واحد كان مسؤول عن الـ specialized
+
+236
+00:18:18,360 --> 00:18:20,860
+transduction اللي هو التحتاني والفجر اللي كان 
+
+237
+00:18:20,860 --> 00:18:23,520
+مسؤول عن مين؟ عن الـ generalized transduction
+
+238
+00:18:26,640 --> 00:18:30,300
+بنقدر نلخص الـ life cycle بـ 4 خطوات رئيسية
+
+239
+00:18:30,300 --> 00:18:34,120
+absorption أو attachment absorption أو attachment
+
+240
+00:18:34,120 --> 00:18:37,840
+بعدين penetration للاختراق بعدين الـ biological
+
+241
+00:18:37,840 --> 00:18:42,360
+synthesis وكلمة biological synthesis جملة بسيطة
+
+242
+00:18:42,360 --> 00:18:46,600
+لكن فيها تفاصيل ضخمة جدا محاولوشها لما اتكلم عن
+
+243
+00:18:46,600 --> 00:18:50,620
+الفيروسات وهتكلم عن الـ viral replication هلاجي
+
+244
+00:18:50,620 --> 00:18:55,140
+حالة أن أكبر جزء اللي احنا مختصرينه بجملة واحدة
+
+245
+00:18:55,140 --> 00:18:58,040
+اللي هو biological synthesis فده ينطق الجملة
+
+246
+00:18:58,040 --> 00:19:05,300
+الأخيرة maturation أو assembly إيش مادة .. إيش
+
+247
+00:19:05,300 --> 00:19:09,800
+مادة maturation نضوج .. نضوج أو assembly واللي هو
+
+248
+00:19:09,800 --> 00:19:15,760
+الـ release الخطوة الأولى عسارية كارتون هي عبارة عن
+
+249
+00:19:15,760 --> 00:19:21,160
+فقط ارتباط بالـ receptors وسميناها absorption أو
+
+250
+00:19:21,160 --> 00:19:25,530
+attachment الخطوة الثانية الـ penetration لاحظوا أن 
+
+251
+00:19:25,530 --> 00:19:29,670
+الـ viral genome دخل إلى داخل الخلية لكن الفيروس
+
+252
+00:19:29,670 --> 00:19:34,130
+نفسه الهيكل الخارجي بيبقى بالخارج هذا طبعًا اعتبره
+
+253
+00:19:34,130 --> 00:19:38,070
+صدفة خالية من الحياة خاصة يعني ما لوش أي قيمة
+
+254
+00:19:38,070 --> 00:19:42,390
+ما لوش أي قيمة الخطوة الثالثة الـ biological
+
+255
+00:19:42,390 --> 00:19:45,910
+synthesis بيتصنع اللي هي الفيروسات كاملة بعد ذلك 
+
+256
+00:19:45,910 --> 00:19:46,210
+اللي هو
+
+257
+00:19:51,120 --> 00:19:55,480
+اللي قدامكم الآن هي عبارة عن تلخيص لكل العملية الـ
+
+258
+00:19:55,480 --> 00:19:58,320
+bacteriophage بيعمل attachment أو بناء absorption
+
+259
+00:19:58,320 --> 00:20:02,660
+بعدين penetration
+
+260
+00:20:02,660 --> 00:20:05,320
+للمادة الوراثية بعدين اللي هو biological
+
+261
+00:20:05,320 --> 00:20:10,720
+synthesis, maturation and release وهذه العملية أدت
+
+262
+00:20:10,720 --> 00:20:17,120
+إلى تحلل الخلايا الخلايا البكتيرية وإطلاق الفيروس
+
+263
+00:20:18,220 --> 00:20:22,180
+الآن امرأة بأننا نستغل هذه المعلومات أو هذه
+
+264
+00:20:22,180 --> 00:20:27,060
+المعلومات في تطبيق مهم جدًا في الـ microbiology وهو
+
+265
+00:20:27,060 --> 00:20:34,260
+عد الفيروسات من هذا النوع إحنا زي ما بنشتري منتجات
+
+266
+00:20:34,260 --> 00:20:38,760
+كثيرة بنشتري فيروسات و بنشتري بكتيريا و بنبيع و
+
+267
+00:20:38,760 --> 00:20:44,230
+بنشتريها ولما بنبيع بنقول أنا ببيع لك مليون فيروس
+
+268
+00:20:44,230 --> 00:20:49,330
+أو ببيع لك أمبولة فيها مليار فيروس فأنا بتأكد من هذا
+
+269
+00:20:49,330 --> 00:20:54,470
+أو بدي أصنع رقم معين ففي الآخر بدي أعد يعني بيلزمنا
+
+270
+00:20:54,470 --> 00:21:00,590
+أعمل count بيلزمنا نعد الـ bacteriophages كيك ممكن
+
+271
+00:21:00,590 --> 00:21:03,250
+أعد الـ bacteriophages اللي من هذا النوع اللي هي الـ
+
+272
+00:21:03,250 --> 00:21:08,690
+lytic phages ركزوا معاه عشان نوضح الصورة بشكل جيد
+
+273
+00:21:10,160 --> 00:21:13,160
+تخيلوا معايا طبق.. طبق اللي في المعمل، شفتوه؟ و
+
+274
+00:21:13,160 --> 00:21:14,200
+لا.. و لا.. و لا.. و لا.. و لا.. و لا.. و لا
+
+275
+00:21:14,200 --> 00:21:14,260
+.. و لا.. و لا.. و لا.. و لا.. و لا.. و لا.. 
+
+276
+00:21:14,260 --> 00:21:14,420
+و لا.. و لا.. و لا.. و لا.. و لا.. و لا.. و
+
+277
+00:21:14,420 --> 00:21:14,800
+لا.. و لا.. و لا.. و لا.. و لا.. و لا.. و لا
+
+278
+00:21:14,800 --> 00:21:14,820
+.. و لا.. و لا.. و لا.. و لا.. و لا.. و لا..
+
+279
+00:21:14,820 --> 00:21:15,600
+و لا.. و لا.. و لا.. و لا.. و لا.. و لا.. و
+
+280
+00:21:15,600 --> 00:21:17,080
+لا.. و لا.. و لا.. و لا.. و لا.. و لا.. و لا
+
+281
+00:21:17,080 --> 00:21:17,760
+.. و لا.. و لا.. و لا.. و لا.. و لا.. و لا..
+
+282
+00:21:17,760 --> 00:21:17,780
+و لا.. و لا.. و لا.. و لا.. و لا.. و لا.. و
+
+283
+00:21:17,780 --> 00:21:22,600
+لا.. و لا.. و لا.. و لا.. و لا.. و لا.. و لا
+
+284
+00:21:22,600 --> 00:21:28,180
+.. و لا.. و لا.. 
+
+285
+00:21:38,920 --> 00:21:41,520
+الأمبولة اللي فيها الـ bacteriophage و بنعدها
+
+286
+00:21:41,520 --> 00:21:44,940
+بنعملها dilution تخيلوا ولا بنتكلم عن مليار ولا
+
+287
+00:21:44,940 --> 00:21:50,840
+مليون لو قلت لك قد إيه من واحد لمليار هتموتي وأنت
+
+288
+00:21:50,840 --> 00:21:53,960
+تقولي تسعمية وتسعة وخمسين ألف يمكن أو.. يعني مش
+
+289
+00:21:53,960 --> 00:21:59,790
+هنخلص ولا لأ؟ فإيش هنعمل؟ هنعمل dilution وعمل الـ
+
+290
+00:21:59,790 --> 00:22:03,250
+dilution بيعتمد على الـ original number على الـ
+
+291
+00:22:03,250 --> 00:22:05,570
+original number قد إيه الـ original number اللي موجود
+
+292
+00:22:05,570 --> 00:22:11,010
+مش عارفينه هنا إذا متوقع ممكن نعمل مثلًا dilution
+
+293
+00:22:11,010 --> 00:22:14,430
+كتير إذا عارفين بالضبط الـ original بنعمل dilution
+
+294
+00:22:14,430 --> 00:22:19,490
+على جده بحاجة إنه في الآخر نحصل على 100 فيروس إلى
+
+295
+00:22:19,490 --> 00:22:25,410
+200 فيروس تمام؟ نقدر نقدهم مظبوط؟ عملنا dilution
+
+296
+00:22:25,410 --> 00:22:29,690
+في القنبوبة ومن القنبوبة الأخيرة أخذنا واحد مل و
+
+297
+00:22:29,690 --> 00:22:34,850
+فرضناه على البكتيريا، الآن الواحد مل نفترض..
+
+298
+00:22:34,850 --> 00:22:40,890
+نفترض طلع على الطبق تاني يوم خمسين
+
+299
+00:22:40,890 --> 00:22:44,710
+.. خمسين فراغ، إيش هنا خمسين فراغ؟
+
+300
+00:22:55,280 --> 00:22:58,160
+هيقرصهم بس شوية لو طلبنا إنه البكتيريا كيف نامت
+
+301
+00:22:58,160 --> 00:23:06,680
+البكتيريا مش شايفينها؟ في
+
+302
+00:23:06,680 --> 00:23:12,180
+لومباد صح؟ على السبورة، هلأ تقولوا نمو البكتيريا،
+
+303
+00:23:12,180 --> 00:23:15,080
+لو كان فيه بكتيريو فاج المنطقة هالي، إيش هيعمل
+
+304
+00:23:15,080 --> 00:23:18,600
+البكتيريا قلناها؟ هيحللها، هيحللها وهينتقل لخلايا
+
+305
+00:23:18,600 --> 00:23:24,280
+مجاورة، هيحللها، فبالتالي هلاقي منطقة يشملها هذه
+
+306
+00:23:24,280 --> 00:23:28,140
+المنطقة الفراغات بنسميها plaque، plaque منطقة خالية
+
+307
+00:23:28,140 --> 00:23:33,200
+من النمو، نتيجة تحلل البكتيريا من قبل الفيروس،
+
+308
+00:23:33,200 --> 00:23:37,180
+مثلًا هو موضح في هذه الصورة ده، تشوفونا أمامكم،
+
+309
+00:23:37,180 --> 00:23:41,160
+موضح في الصورة هذه إنه في نمو وبعدين في الناقل هو
+
+310
+00:23:41,160 --> 00:23:46,580
+الفراغات، إذا لو عدينا خمسين فراغات، خمسين فراغات
+
+311
+00:23:46,580 --> 00:23:52,230
+من أي dilution من الأخير مل افترض إنه كان عشرة
+
+312
+00:23:52,230 --> 00:23:57,170
+أس ناقص ستة عشرة أس ناقص ستة خمسين ضرب عشرة أس
+
+313
+00:23:57,170 --> 00:24:01,890
+ستة بيطلع الرقم الحقيقي تبعنا إيه الوحدات اللي
+
+314
+00:24:01,890 --> 00:24:08,170
+بنحطها؟ بنحط وحدة هذا في البكتيريا لما نقعد في
+
+315
+00:24:08,170 --> 00:24:11,290
+البكتيريا colony forming unit لكن في الفيروسات
+
+316
+00:24:11,290 --> 00:24:16,930
+بنسميها PFU plaque forming unit PFU
+
+317
+00:24:20,340 --> 00:24:29,500
+ليه ما قلناش الوحدات bacteriophage ليه بنقول BFU
+
+318
+00:24:29,500 --> 00:24:33,920
+plaque Forming Unit خدوا بالكم النقطة إحنا عدينا
+
+319
+00:24:33,920 --> 00:24:39,000
+bacteriophage ولا عدينا plaques طيب إحنا الـ plaques
+
+320
+00:24:39,000 --> 00:24:45,180
+بنفترض إن كل plaque ناشئ عن عن فيروس واحد عن فيروس
+
+321
+00:24:45,180 --> 00:24:49,610
+واحد هل هذا الافتراض حقيقي؟ طب لو كانوا اثنين اللي
+
+322
+00:24:49,610 --> 00:24:54,670
+ولزقوا مع بعض وعملونا plaque واحد فعشان أنا أدعي
+
+323
+00:24:54,670 --> 00:24:58,050
+الدقة في الـ micro biology فبروح وأنا حاطط بالفعل
+
+324
+00:24:58,050 --> 00:25:03,310
+وصف لما قمت بعمله بدل ما أقول خمسين ضرب عشرة لقوة
+
+325
+00:25:03,310 --> 00:25:09,010
+ستة viruses أو بكتيريو فاج بقول خمسين ضرب عشرة لقوة
+
+326
+00:25:09,010 --> 00:25:13,490
+ستة plaque forming unit الـ unit ممكن تكون virus
+
+327
+00:25:13,490 --> 00:25:15,850
+واحد ممكن ما تكونش فعلًا virus واحد لكن في العالم
+
+328
+00:25:15,850 --> 00:25:21,840
+الغالب في الـ micro virus بينظروا إنها كل plaque إنه
+
+329
+00:25:21,840 --> 00:25:26,740
+virus طب هذه النقطة مهمة جدًا من النقطة هذه إن إحنا
+
+330
+00:25:26,740 --> 00:25:31,000
+نعد الفيروسات مهم جدًا إن نعد الفيروسات زي ما مهم
+
+331
+00:25:31,000 --> 00:25:36,200
+جدا إن نعد البكتيريا هذا سؤال لو سأله حطيته
+
+332
+00:25:36,200 --> 00:25:40,660
+وسؤال نقاش لماذا ما هي تطبيقات لعد الفيروسات
+
+333
+00:25:40,660 --> 00:25:44,220
+أرجو منكم تتفاعلوا علشان الآن طبعًا على الموقع
+
+334
+00:25:44,220 --> 00:25:47,540
+الفيسبوك إيش النقاط اللي ممكن نستفيد منها في عد
+
+335
+00:25:47,540 --> 00:25:56,460
+اللي هو الفيروسات النوع
+
+336
+00:25:56,460 --> 00:25:59,940
+الثاني من الفيروسات المعنونة اللي هو الـ الـ الـ الـ
+
+337
+00:25:59,940 --> 00:26:04,230
+lysogenic أو الـ temperate برضه attachment أو
+
+338
+00:26:04,230 --> 00:26:08,690
+absorption penetration
+
+339
+00:26:08,690 --> 00:26:17,330
+الآن لأ الآن هيعملش الآن integration صح بروفيج
+
+340
+00:26:17,330 --> 00:26:19,970
+formation اللي يسمى إنها integration و الفيروس
+
+341
+00:26:19,970 --> 00:26:24,810
+بيقول للـ DNA أنا بدي أ.. بس ما كان المفروض إن
+
+342
+00:26:24,810 --> 00:26:28,070
+البكتيريا في هذه اللحظة إذا بتنقسم.. إذا بتنقسم
+
+343
+00:26:28,070 --> 00:26:34,000
+هينقسم معاها تمام؟ ملخص العملية إنه يكون بروفيج بس
+
+344
+00:26:34,000 --> 00:26:41,080
+لحد الآن تكون بروفيج نكمل الآن الفيروس قرر لسبب ما
+
+345
+00:26:41,080 --> 00:26:46,100
+إنه ينفصل ينسحب أنا قلت لكم السبب.. السبب ما هذا
+
+346
+00:26:46,100 --> 00:26:50,460
+في الغالب إيه induction إيش الـ induction يعني في
+
+347
+00:26:50,460 --> 00:26:55,400
+عوامل معروفة في الـ micro biology مثل الفيروس منها
+
+348
+00:26:55,400 --> 00:27:01,640
+شؤون الفيروسالشهور كلمة شهور مش يعني اللي هتطبق
+
+349
+00:27:01,640 --> 00:27:06,260
+على الفيروسات لكن خلينا نقول قايلة وميكانيزم by
+
+350
+00:27:06,260 --> 00:27:09,580
+which the virus senses that the bacteria is dying
+
+351
+00:27:09,580 --> 00:27:15,500
+البكتيريا تقرض للموضوع، بينفصل طبعًا في الآخر برجع
+
+352
+00:27:15,500 --> 00:27:19,360
+وبيكون نفس الفكرة اللي عملها الـ lytic phage
+
+353
+00:27:19,360 --> 00:27:22,140
+بيعمل الآن biological synthesis اللي هي قدام
+
+354
+00:27:22,140 --> 00:27:25,400
+العملية وبعد ذلك اللي هي الـ release
+
+355
+00:27:28,890 --> 00:27:32,490
+لنفس البكتيريا لأ مش هو سحب الـ .. الـ .. الـ .. الـ
+
+356
+00:27:32,490 --> 00:27:32,750
+.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ
+
+357
+00:27:32,750 --> 00:27:33,230
+الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ
+
+358
+00:27:33,230 --> 00:27:34,670
+.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ
+
+359
+00:27:34,670 --> 00:27:37,770
+.. الـ .. الـ
+
+360
+00:27:37,770 --> 00:27:41,430
+الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ
+
+361
+00:27:41,430 --> 00:27:41,490
+.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ
+
+362
+00:27:41,490 --> 00:27:41,650
+الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ
+
+363
+00:27:41,650 --> 00:27:42,950
+.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ
+
+364
+00:27:42,950 --> 00:27:43,170
+الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ
+
+365
+00:27:43,170 --> 00:27:44,210
+الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ
+
+366
+00:27:44,210 --> 00:27:54,370
+.. الـ .. الـ .. الـ .. الـ .. الـ
+
+367
+00:28:05,460 --> 00:28:07,900
+الآن خلق.. هي كده.. هي كده الـ profile ولا يزور
+
+368
+00:28:07,900 --> 00:28:12,020
+نوع الـ cell قرر إنه ينفصل في نفس البكتيريا عمل
+
+369
+00:28:12,020 --> 00:28:18,320
+biological synthesis و
+
+370
+00:28:18,320 --> 00:28:21,120
+بعدين صار له maturation وبعدين بيصير له اللي هو
+
+371
+00:28:21,120 --> 00:28:25,200
+release يبقى العملية بالكامل كلها في بكتيريا طبعًا
+
+372
+00:28:25,200 --> 00:28:27,040
+البكتيريا الواحدة أقصد فيها اللي هي الـ .. الـ
+
+373
+00:28:27,040 --> 00:28:29,640
+progeny اللي هي السلالة اللي نشأت من الـ .. من
+
+374
+00:28:29,640 --> 00:28:31,440
+الخلية الأولى الـ original cell
+
+375
+00:28:36,610 --> 00:28:40,410
+What are the applications of bacteriophages؟ واحد،
+
+376
+00:28:40,410 --> 00:28:42,430
+وحكيناهم كلها خذوا بالكم، بس بدنا نعيدهم الآن
+
+377
+00:28:42,430 --> 00:28:45,990
+وندكركمهم بشكل نقاط، واحد، treatment of bacterial 
+
+378
+00:28:45,990 --> 00:28:54,230
+infection، وأنا بقول لكم يعني نموذج يمكن قديم جدا،
+
+379
+00:28:54,230 --> 00:28:58,990
+الموضوع مش جديد، الروس أو في روسيا اهتموا بهذا
+
+380
+00:28:58,990 --> 00:29:02,350
+الموضوع منذ أو أول القرن العشرين، يعني في ال
+
+381
+00:29:02,350 --> 00:29:06,780
+ألف وتسعمائة وإشي، بدؤوا يهتموا بهذا الموضوع اكتشاف ال
+
+382
+00:29:06,780 --> 00:29:13,020
+antibiotics في أربعينيات القرن الماضي خلى العالم 
+
+383
+00:29:13,020 --> 00:29:16,940
+الغربي ينسى موضوع الviruses ويركز على موضوع ال
+
+384
+00:29:16,940 --> 00:29:22,360
+antibiotics لأن ال antibiotics بالفعل كانت طفرة
+
+385
+00:29:22,360 --> 00:29:25,700
+يعني لحد .. ل .. ل .. ل .. لدرجة أنه المؤرخين
+
+386
+00:29:25,700 --> 00:29:30,580
+الطبيين يسمون عصر الماضي .. القرن الماضي عصر
+
+387
+00:29:30,580 --> 00:29:36,140
+المغضب الحيوي ليش؟ لأنه أحدثت قفزة في ال .. في
+
+388
+00:29:36,140 --> 00:29:40,660
+العلاجات وفي الطب احنا دلوقتي كانوا بيموتوا من ..
+
+389
+00:29:40,660 --> 00:29:43,800
+من simple .. من simple tonsillitis أو urinary
+
+390
+00:29:43,800 --> 00:29:46,900
+tract infection اليوم لأ ال antibiotics بالفعل
+
+391
+00:29:46,900 --> 00:29:50,420
+قللت من نسبة الوفيات في أمراض كتيرة حسنت من
+
+392
+00:29:50,420 --> 00:29:53,900
+العمليات، نجاح العمليات الجراحية لأنه فجأة زمان
+
+393
+00:29:53,900 --> 00:29:57,680
+تنجح العملية ويموت المريض من التهابات بعد
+
+394
+00:29:57,680 --> 00:30:01,880
+العملية فال antibiotic أحدثت قفزة كبيرة فالعالم
+
+395
+00:30:01,880 --> 00:30:05,400
+الغربي تقريبا نسي موضوع ال bacteriophages وركز كله
+
+396
+00:30:05,400 --> 00:30:09,500
+على مين، لكن الروس ضالهم شغالين، لكن كونوا الروس
+
+397
+00:30:09,500 --> 00:30:13,700
+.. عمركم قرأتوا مقالة بالروس ينتوا مثلا؟ لأ ليش؟
+
+398
+00:30:13,700 --> 00:30:16,220
+لأن اللغة تبعتهم لم تنتشر كما انتشرت اللغة
+
+399
+00:30:16,220 --> 00:30:20,200
+الإنجليزية في العالم، الآن وبعد ظهور سلالات مقاومة
+
+400
+00:30:20,200 --> 00:30:26,480
+للمضادات الحيوية و بوادر أنه احنا خسرين المعركة مع
+
+401
+00:30:26,480 --> 00:30:30,160
+المضادات الحيوية، بدأ العالم الغربي مرة أخرى يعيد
+
+402
+00:30:30,160 --> 00:30:35,110
+الاهتمام بموضوع اللي هو ال bacteriophage وفيه زميل
+
+403
+00:30:35,110 --> 00:30:39,590
+أو دكتورة من السعودية أعتقد أنها فلسطينية من غزة
+
+404
+00:30:39,590 --> 00:30:44,630
+من هنا عملت دراسة على bacteriophage لـ عزلت
+
+405
+00:30:44,630 --> 00:30:47,790
+bacteriophage لقتل واستخدموه في العلاج في السعودية
+
+406
+00:30:47,790 --> 00:30:52,130
+لقتل ال methicillin resistance Staphylococcus aureus
+
+407
+00:30:52,130 --> 00:30:55,610
+ونجحوا في ذلك وكانت هذه رسالة دكتوراه بهذه
+
+408
+00:30:55,610 --> 00:30:58,590
+الدكتوراه ففي عندهم potential
+
+409
+00:31:02,360 --> 00:31:07,720
+مستقبلية لاستخدام وتوظيف البكتيروفاجز في علاج بعض
+
+410
+00:31:07,720 --> 00:31:14,340
+الأمراض وهم يميزها كمان مرة لـ specificity يعني
+
+411
+00:31:14,340 --> 00:31:18,480
+بنقتل السلمونيلا دون أن نضرب ال normal flora أنت
+
+412
+00:31:18,480 --> 00:31:22,860
+بتتكلم أحد عيوبها كمان أنها بتقتل ال pathogen وال
+
+413
+00:31:22,860 --> 00:31:26,340
+monkey pathogen وهذا بيملي خلل في مين؟ في ال
+
+414
+00:31:26,340 --> 00:31:30,330
+normal flora هذل على إنه هذا .. إنه .. إنه ال
+
+415
+00:31:30,330 --> 00:31:33,270
+bacterial phage يعني ما عندهوش أي عيوب، لأ أحد
+
+416
+00:31:33,270 --> 00:31:36,210
+عيوب، واللي حكينا اللي أنا هجيبه شوية، إنه لو دخل
+
+417
+00:31:36,210 --> 00:31:39,670
+رسم الإنسان في الدم يا ماما، رسم الإنسان بتعامل
+
+418
+00:31:39,670 --> 00:31:43,310
+فعليا كرسم غريب و بي .. بيكسره، وبالتالي مش هيكون
+
+419
+00:31:43,310 --> 00:31:48,690
+فعال في علاج كل أنواع ال infection في رسم الإنسان،
+
+420
+00:31:48,690 --> 00:31:53,160
+نمر اثنين في الفوائد أو التطبيقات used in the
+
+421
+00:31:53,160 --> 00:31:57,040
+identification of pathogenic bacteria ومصطلح سمنها
+
+422
+00:31:57,040 --> 00:32:03,380
+phage أو phage type كونه بينقل جينات عفوا من خلية
+
+423
+00:32:03,380 --> 00:32:07,440
+إلى خلية فقد يستخدم في ال molecular biology
+
+424
+00:32:07,440 --> 00:32:08,140
+research
+
+425
+00:32:20,350 --> 00:32:23,130
+لحظة يا أفندي، اللي هو حادة، بس لحظة اللي هي الـ
+
+426
+00:32:23,130 --> 00:32:25,790
+lysogenized أو الـ lysogenic conversion احنا
+
+427
+00:32:25,790 --> 00:32:30,510
+اتفقنا على إنه وجود prophage في البكتيريا بيعطي
+
+428
+00:32:30,510 --> 00:32:35,830
+البكتيريا اسم lysogenized bacteria الآن هل لل
+
+429
+00:32:35,830 --> 00:32:40,350
+lysogenized bacteria أي أهمية في ال medical
+
+430
+00:32:40,350 --> 00:32:45,170
+microbiology؟ نعم، هناك أهمية كبرى، لهذا المصطلح
+
+431
+00:32:45,170 --> 00:32:48,490
+في ال medical microbiology وأنا ذكرت مثلًا المرة
+
+432
+00:32:48,490 --> 00:32:52,720
+الماضية فاكرينه ولا مش فاكرينه؟ اللي هو ال
+
+433
+00:32:52,720 --> 00:32:57,920
+Corynebacterium diphtheriae هذه بكتيريا غير سامة لما
+
+434
+00:32:57,920 --> 00:33:03,680
+بيصير lysogenized بالفعش تبعها بتنتج توكسينو سموها
+
+435
+00:33:03,680 --> 00:33:07,300
+toxigenic Corynebacterium diphtheriae ولو عزلت
+
+436
+00:33:07,300 --> 00:33:10,500
+Corynebacterium diphtheriae من المريض لأ وعرفتها بيو
+
+437
+00:33:10,500 --> 00:33:13,640
+كيميائية عنها Corynebacterium diphtheriae لازم أتأكد
+
+438
+00:33:13,640 --> 00:33:18,990
+منها هل هي toxigenic ولا non-toxigenic هي أهمية لل
+
+439
+00:33:18,990 --> 00:33:24,130
+Lysogenic Conversion وهناك ميكروبات أخرى زي ال
+
+440
+00:33:24,130 --> 00:33:27,030
+Clostridium botulinum اللي بتنتج أقوى سم عارف
+
+441
+00:33:27,030 --> 00:33:31,550
+للإنسان أيضا هي ناتجة عن Lysogenic Conversion
+
+442
+00:33:31,550 --> 00:33:37,910
+ببكتيريو فاج طب بتعطيكوا كمان واحدة خطيرة جدا هل
+
+443
+00:33:37,910 --> 00:33:43,010
+سمعتوا ببكتيريا أكلت اللحم؟ ايش اسمها يعني؟
+
+444
+00:33:43,010 --> 00:33:43,470
+كل خير
+
+445
+00:33:46,600 --> 00:33:49,560
+هذه البكتيريا لو أصابت على سبيل المثال كتف واحد
+
+446
+00:33:49,560 --> 00:33:55,880
+ممكن بعد يوم من infection نشوف العظم هي مش بتاكل
+
+447
+00:33:55,880 --> 00:33:59,720
+لحم بمعنى بتاكل لحم وإنما عندها المقدرة على هضم
+
+448
+00:33:59,720 --> 00:34:04,780
+البروتينات بشكل مش طبيعي عندها proteinases كمية
+
+449
+00:34:04,780 --> 00:34:10,680
+proteinases كبيرة جدا وبالتالي قدرتها على هضم
+
+450
+00:34:10,680 --> 00:34:13,980
+البروتينات وتذكروا أن ال muscle سبعتنا أو ال flesh
+
+451
+00:34:13,980 --> 00:34:18,960
+سبعتنا هو أيضا عن بروتين فعطاها قدرة والبكتيريا
+
+452
+00:34:18,960 --> 00:34:23,560
+هذه مشهورة جدا جدا جدا وبتصيبنا احنا باستمرار
+
+453
+00:34:23,560 --> 00:34:28,200
+اسمها Streptococcus pyogenes تماما لواز مين منكم
+
+454
+00:34:28,200 --> 00:34:32,760
+ما قصبش من لواز في يوم من أيام؟ ايه اللي طبعا
+
+455
+00:34:32,760 --> 00:34:36,380
+انشالت لوازه؟ أكيد انشالوها عشان .. عشان بتنصب
+
+456
+00:34:36,380 --> 00:34:39,700
+باستمرار لكن هذه البكتيريا أُفرِجت عن البكتيريا
+
+457
+00:34:39,700 --> 00:34:44,000
+الأخرى أنه صار لها lysogenic conversion ببكتيريو فاج
+
+458
+00:34:44,000 --> 00:34:48,070
+أعطاها المقدرة على إنتاج مين؟ اللي هو ال ال ال ال
+
+459
+00:34:48,070 --> 00:34:48,870
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+460
+00:34:48,870 --> 00:34:49,730
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+461
+00:34:49,730 --> 00:34:51,130
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+462
+00:34:51,130 --> 00:34:52,150
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+463
+00:34:52,150 --> 00:34:53,710
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+464
+00:34:53,710 --> 00:34:59,190
+ال ال ال ال
+
+465
+00:34:59,190 --> 00:35:00,570
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+466
+00:35:00,570 --> 00:35:02,070
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+467
+00:35:02,070 --> 00:35:02,150
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+468
+00:35:11,480 --> 00:35:16,820
+خليةتين ببعضهم البعض على أما الحصول على خلية واحدة
+
+469
+00:35:16,820 --> 00:35:20,860
+تحتوي على خصائص من الخليتين التانية هعطيكوا مثال
+
+470
+00:35:20,860 --> 00:35:26,020
+على protoplast fusion خارج نطاق البكتيريا بتسمعوا
+
+471
+00:35:26,020 --> 00:35:32,200
+بال antibody وبتسمعوا بال monoclonal antibody ال
+
+472
+00:35:32,200 --> 00:35:36,520
+monoclonal
+
+473
+00:35:36,520 --> 00:35:40,420
+antibody هي عبارة عن antibody موجهة ضد EV2 بواحد
+
+474
+00:35:40,420 --> 00:35:46,110
+فقط لها Epitope يعني جزء من أنتجين، مش أنتجين، جزء من
+
+475
+00:35:46,110 --> 00:35:50,510
+أنتجين يسمى محددات الانتجين، antigen determinants
+
+476
+00:35:50,510 --> 00:35:55,150
+أو Epitope هذا الكلام في الجسم مستحيل نحصل على
+
+477
+00:35:55,150 --> 00:36:00,650
+monoclonal الانتيجين بدخل جسم يكون له أجسام مضادة،
+
+478
+00:36:00,650 --> 00:36:03,970
+مش جسم واحد، مش monoclonal، مش سلالة واحدة،
+
+479
+00:36:03,970 --> 00:36:09,950
+سلالات، لأن الانتيجين بيكون أكثر من Epitope ففي
+
+480
+00:36:09,950 --> 00:36:13,950
+الخارج من الجسم بنعمل تقنية اسمها hybridoma
+
+481
+00:36:13,950 --> 00:36:18,050
+technique hybridoma كلمة hybrid ايش يعني hybrid؟
+
+482
+00:36:18,050 --> 00:36:25,350
+تهجين بنجيب خلية سرطانية وبنجيب B lymphocyte ال B
+
+483
+00:36:25,350 --> 00:36:28,590
+lymphocyte دي بنكون اختارناها احنا وعارفين انها
+
+484
+00:36:28,590 --> 00:36:32,330
+تنتج هذا ال antibody اللي بدنا هيها عارفين كده ايش
+
+485
+00:36:32,330 --> 00:36:37,030
+عمرها ال B lymphocyte؟ ساعات، ست ساعات، خارج الجسم
+
+486
+00:36:37,030 --> 00:36:42,730
+وبعدين بتموت hybridoma أو carcinoma cell بتعيش
+
+487
+00:36:42,730 --> 00:36:45,810
+forever وافريلها اكل وشرب وجي معاها ال waste
+
+488
+00:36:45,810 --> 00:36:50,610
+تمام؟ بتعيش forever يبقى ايش الميزة بتاعتها؟ ال
+
+489
+00:36:50,610 --> 00:36:56,570
+survival تبعها forever الثانية short-lived ايش
+
+490
+00:36:56,570 --> 00:36:59,170
+ميزتها ال short-lived؟ انها بتنتج normal antibody
+
+491
+00:36:59,170 --> 00:37:03,660
+أنا بديها الثانية بتنتج normal antibody يبقى في
+
+492
+00:37:03,660 --> 00:37:07,800
+واحدة فيها ميزة جيدة وفيها قيب والثانية فيها قيب
+
+493
+00:37:07,800 --> 00:37:11,900
+وفيها ورضه ميزة، روحت نفشتهم مع بعض، fused in
+
+494
+00:37:11,900 --> 00:37:15,740
+a glycol، فاكريني ما حكينا المرة الماضية، ال pig،
+
+495
+00:37:15,740 --> 00:37:20,710
+فطلعولي خلية اسمها ال hybridoma cell بتعيش forever و
+
+496
+00:37:20,710 --> 00:37:24,810
+بتنتج ل normal antibodies والآن معظم ال reagents
+
+497
+00:37:24,810 --> 00:37:27,730
+اللي بتستخدموهم في المختبرات وفيها antibodies
+
+498
+00:37:27,730 --> 00:37:32,110
+تمام وخذوا بالكوا هذه التقنية اللي هي تقنية أن
+
+499
+00:37:32,110 --> 00:37:35,610
+أنا أستخدم antibody للكشف أو لقياس أشياء متوفرة
+
+500
+00:37:35,610 --> 00:37:39,590
+عندنا في التحيات الطبية بكمية كبيرة أنواع كثيرة من
+
+501
+00:37:39,590 --> 00:37:44,710
+التحوصات متبنية على هذه التقنية فاللي بنجح إنه يعمل
+
+502
+00:37:44,710 --> 00:37:48,850
+hybridoma تقنية .. the hybridoma for Antibody، بيصير عنده
+
+503
+00:37:48,850 --> 00:37:52,590
+مصنع بينتج من خلاله الـ antibodies و بيوزعه على
+
+504
+00:37:52,590 --> 00:37:54,730
+الشركات اللي بتستغل هذا الـ antibody في الـ
+
+505
+00:37:54,730 --> 00:37:57,430
+diagnostic أو في الـ treatment أو في خلاله، إذا الـ
+
+506
+00:37:57,430 --> 00:38:02,470
+Protoplastin فيه أيضا تقنية جيدة و ممتازة، السؤال ..
+
+507
+00:38:02,470 --> 00:38:06,390
+السؤال الـ .. الـ .. المحاضرة اللي هو how could we
+
+508
+00:38:06,390 --> 00:38:08,670
+benefit from bacterial genetics أنا لا أقصد الـ
+
+509
+00:38:08,670 --> 00:38:11,430
+bacterial genetics الـ .. الـ .. الـ .. نفسها إنه
+
+510
+00:38:11,430 --> 00:38:14,730
+المحاضرة تبعتنا، محاضرة الـ bacterial genetics كيف
+
+511
+00:38:14,730 --> 00:38:18,330
+أنا أستفيد من هذه المحاضرة، المحاضرة القادمة إن شاء
+
+512
+00:38:18,330 --> 00:38:26,790
+الله هتكون عن موضوع نقدم له
+
+513
+00:38:26,790 --> 00:38:30,790
+بس تقديم بسيط صغير اسمه sterilization and disease
+
+514
+00:38:33,950 --> 00:38:36,810
+تحت عنوان بنقدر نسميه احنا control of
+
+515
+00:38:36,810 --> 00:38:41,950
+microorganism، خدوا بالكم، علمنا كيف نربيها، نربي
+
+516
+00:38:41,950 --> 00:38:45,910
+البكتيريا نميها، نحط لها الغذاء و الـ pH
+
+517
+00:38:45,910 --> 00:38:49,770
+مناسب و حرارة مناسبة و أكسوجين و CO2 و إلا .. الآن
+
+518
+00:38:49,770 --> 00:38:55,270
+صار عندنا ما عارفة بتنميتها، تنميتها فيش أسهل منها
+
+519
+00:38:55,270 --> 00:38:59,890
+المشكلة هو يعني تسيطر عليها، تخيلوا الغرفة دي اللي
+
+520
+00:38:59,890 --> 00:39:03,270
+احنا قاعدين فيها بدنا نتخلص من كل الميكروبات هذه
+
+521
+00:39:03,270 --> 00:39:08,750
+مهمة صعبة جدا جدا جدا فبدنا نتعلم الطرق المستخدمة
+
+522
+00:39:08,750 --> 00:39:12,310
+في التعقيم و التطهير و دايما كلمة تعقيم مصطلح
+
+523
+00:39:12,310 --> 00:39:16,490
+بنسميه احنا absolute، ايش أنت absolute؟ مطلق والـ
+
+524
+00:39:16,490 --> 00:39:20,190
+absolute killing of all forms of life فده بدنا
+
+525
+00:39:20,190 --> 00:39:24,700
+نعقم الـ إيد، التعقيم .. كلمة التعقيم قلنا absolute
+
+526
+00:39:24,700 --> 00:39:29,400
+killing of all forms of life قد نستطيع أننا نعقمها
+
+527
+00:39:29,400 --> 00:39:33,380
+و نحطها في فرن على 170 درجة مئوية لمدة ساعتين،
+
+528
+00:39:33,380 --> 00:39:37,940
+هتعقم الـ إيد بس مش هتضل الـ إيد، لمرة واحدة، فهنشوف
+
+529
+00:39:37,940 --> 00:39:41,420
+الطرق المتنوعة و المختلفة و متى بنلجأ للتعقيم و
+
+530
+00:39:41,420 --> 00:39:44,200
+متى بنلجأ للتطهير و التطهير باختلاف في معناه
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/KvmHhaw5nZI_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/KvmHhaw5nZI_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..556f668e662658edc76eae7fa574198c9e8e3637
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/KvmHhaw5nZI_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 4554, "start": 20.64, "end": 45.54, "text": "بسم الله الرحمن الرحيم استكمالا للموضوع اللى بدأناه المحاضرة السابقة اللى هو ال bacterial genetics هنمره مره سريع على كل النقاط اللى مرناها المحاضرة السابقة لكن بشكل سريع جدا تذكروا أن المادة اللى قرار فيها بالبكتيريا هي واحدة من ثلاثة يا إما كروموزوم يا إما بلازمت يا إما إبيزوم والإبيزوم هو حقا بلازمت يعني يا كروموزوم يا بلازمت في وقتها", "tokens": [3555, 38251, 21984, 34892, 5016, 27842, 34892, 5016, 32640, 44713, 24793, 6027, 995, 5296, 19528, 2407, 11242, 45367, 13672, 7578, 47525, 10721, 8315, 3224, 9673, 5016, 46958, 25720, 21136, 16758, 28671, 13672, 7578, 31439, 2423, 35632, 26516, 8032, 1863, 29973, 3224, 3714, 2288, 3224, 8608, 16572, 3615, 15844, 28242, 28239, 4587, 41193, 13672, 7578, 3714, 2288, 8315, 11296, 9673, 5016, 46958, 25720, 21136, 16758, 28671, 44381, 4724, 8592, 28820, 8608, 16572, 3615, 10874, 28259, 6055, 8848, 37983, 14407, 14739, 9673, 18513, 3660, 13672, 7578, 12174, 2288, 9640, 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على أسماء بكتيريا معروفة أنه قادرة عليها تاخد DNA من ال surrounding واش تملؤ .. تملؤ incorporation أو recombination يعني اللي هو دمجوا في المادة الوراثية تبعها زي البصيلاس و الhemophilus و النسيريا و ال pneumococcus هو اسم آخر لrestrictococcus pneumonia", "tokens": [6027, 9887, 19446, 4386, 19446, 5551, 19913, 1863, 14739, 3224, 5296, 10721, 4032, 3714, 33753, 26108, 5551, 2655, 4117, 19528, 18871, 11296, 9122, 12984, 13546, 995, 44381, 8978, 13672, 2407, 5016, 3660, 13672, 1829, 5551, 2304, 10943, 4117, 14407, 8978, 27188, 4587, 25720, 6024, 104, 7649, 10632, 8978, 5551, 2304, 12984, 37977, 15844, 5551, 38251, 16606, 4724, 4117, 2655, 13546, 25528, 20449, 32887, 5172, 3660, 14739, 3224, 12174, 18513, 25720, 25894, 11296, 6055, 47283, 3215, 8272, 9154, 2423, 11498, 4032, 33599, 46811, 1211, 33604, 4386, 46811, 1211, 33604, 8788, 399, 34051, 850, 3548, 2486, 37495, 22653, 13672, 1829, 31439, 11778, 2304, 7435, 14407, 8978, 9673, 18513, 3660, 2423, 13063, 5718, 104, 10632, 6055, 3555, 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"word": " هو", "probability": 0.96875}, {"start": 747.76, "end": 748.2, "word": " دمجوا", "probability": 0.85791015625}, {"start": 748.2, "end": 748.46, "word": " في", "probability": 0.984375}, {"start": 748.46, "end": 749.18, "word": " المادة", "probability": 0.8946940104166666}, {"start": 749.18, "end": 749.62, "word": " الوراثية", "probability": 0.826953125}, {"start": 749.62, "end": 750.02, "word": " تبعها", "probability": 0.876708984375}, {"start": 750.02, "end": 750.2, "word": " زي", "probability": 0.949951171875}, {"start": 750.2, "end": 750.9, "word": " البصيلاس", "probability": 0.5904296875}, {"start": 750.9, "end": 751.3, "word": " و", "probability": 0.814453125}, {"start": 751.3, "end": 751.98, "word": " الhemophilus", "probability": 0.77080078125}, {"start": 751.98, "end": 752.4, "word": " و", "probability": 0.99267578125}, {"start": 752.4, "end": 753.04, "word": " النسيريا", "probability": 0.65679931640625}, {"start": 753.04, "end": 753.48, "word": " و", "probability": 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من جسم غريب أو من خلية غريبة أو من خلية متبرعة إلى داخل الخلية اللي يسميناها recipient cell طبعا إذا بتقارنه هاي ال original و ال original ده بيسميه البكتيريا ال wild", "tokens": [2407, 39182, 9957, 39894, 25957, 1211, 11296, 8788, 399, 11778, 47283, 1211, 2423, 8272, 6156, 6027, 1867, 8978, 7649, 16254, 8272, 9154, 10874, 38251, 32771, 16572, 3555, 34051, 9154, 16490, 1211, 10632, 32771, 16572, 49401, 34051, 9154, 16490, 1211, 10632, 44650, 26890, 27884, 30731, 11778, 47283, 1211, 33962, 1211, 10632, 13672, 1829, 7251, 38251, 1829, 8315, 11296, 26216, 2815, 23032, 3555, 3615, 995, 11933, 15730, 39894, 4587, 9640, 1863, 3224, 8032, 47302, 2423, 3380, 4032, 2423, 3380, 11778, 3224, 4724, 1829, 38251, 1829, 3224, 29739, 4117, 2655, 13546, 25528, 2423, 4868], "avg_logprob": -0.19446331375966902, "compression_ratio": 1.6822429906542056, "no_speech_prob": 7.152557373046875e-07, "words": [{"start": 768.91, "end": 769.07, "word": "و", "probability": 0.406982421875}, {"start": 769.07, "end": 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\ No newline at end of file
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/LLEOIggQxQs_postprocess.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/LLEOIggQxQs_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..1d387efce10602aae6f870451d65195cd171ae52
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/LLEOIggQxQs_postprocess.srt
@@ -0,0 +1,2804 @@
+1
+00:00:20,430 --> 00:00:23,870
+بسم الله الرحمن الرحيم فصل جديد بعنوان detection,
+
+2
+00:00:24,110 --> 00:00:26,490
+prophylaxis and treatment of bacterial infection
+
+3
+00:00:26,490 --> 00:00:31,590
+قبل ما ابدأ الموضوع بدأ امهد للعنوان انه ليس من
+
+4
+00:00:31,590 --> 00:00:35,110
+وظائفنا احنا في مجلة العلم الطبية المثبرية ان نقوم
+
+5
+00:00:35,110 --> 00:00:38,570
+بالعلاج او يعني
+
+6
+00:00:42,660 --> 00:00:46,620
+ولا ال prophylaxis بينما بالنسبة لل detection لل
+
+7
+00:00:46,620 --> 00:00:51,220
+bacterial infection بينما أن هذا الدور أساسا هو
+
+8
+00:00:51,220 --> 00:00:54,620
+بيساعد في عملية ال diagnosis و بيساعد في عملية ال
+
+9
+00:00:54,620 --> 00:00:58,730
+prophylaxis و بيساعد في عملية ال treatmentهذا يعني
+
+10
+00:00:58,730 --> 00:01:02,970
+انه بيلقي على عتقنا قرابق كبير جدا من حيث انه دقة
+
+11
+00:01:02,970 --> 00:01:07,270
+ال diagnosis لل bacterial infection اليوم اعتقد ان
+
+12
+00:01:07,270 --> 00:01:10,770
+احنا أصبحنا مؤهلين بعد ما درسنا مجموعة من الفصول
+
+13
+00:01:10,770 --> 00:01:15,470
+انه نتكلم عن how bacterial infection are detected
+
+14
+00:01:15,470 --> 00:01:22,630
+in the laboratory هتكلم عن ال process of diagnosis
+
+15
+00:01:22,630 --> 00:01:26,950
+of bacterial infection in generalو هنبدأ ب step
+
+16
+00:01:26,950 --> 00:01:33,930
+number one تذكير دائم بإن عيناتنا ال clinical اللي
+
+17
+00:01:33,930 --> 00:01:38,550
+هي من الإنسان نسميها clinical و عينات من المصاد
+
+18
+00:01:38,550 --> 00:01:41,830
+الأخرى ممكن نسميها environmental يعني ممكن ناخد
+
+19
+00:01:41,830 --> 00:01:47,050
+عينة هوا عينة ميه من البحر من التربة ممكن عينة
+
+20
+00:01:47,050 --> 00:01:50,130
+غذاء نسميها environmental especially إذا نجسمهم من
+
+21
+00:01:50,130 --> 00:01:55,130
+الآن clinical و environmentalو كلهم خدوا فالكوا
+
+22
+00:01:55,130 --> 00:01:58,910
+لإنكوا إذا تذكروا لما درسنا ال infection chain
+
+23
+00:01:58,910 --> 00:02:04,010
+اللي هي سلسلة أو ال cycle وجدنا إنه ال .. ال .. ال
+
+24
+00:02:04,010 --> 00:02:07,410
+carriers ممكن تكون food ممكن تكون ماء ممكن تكون
+
+25
+00:02:07,410 --> 00:02:11,670
+whatsoever حشرات وبالتالي العينات ممكن تكون متنوعة
+
+26
+00:02:11,670 --> 00:02:15,770
+الآن لو دينا تكلمنا عن ال medical technology
+
+27
+00:02:15,770 --> 00:02:19,310
+profession مهنة التحقيق الطبية و تحدثنا عن نوع
+
+28
+00:02:19,310 --> 00:02:22,850
+العينات اللي أنتوا بتتعاملوا معاها معظم الوقتعينات
+
+29
+00:02:22,850 --> 00:02:28,190
+دم عينات urine عينات بعض الحالات body fluid بس في
+
+30
+00:02:28,190 --> 00:02:33,110
+ال microbiology everything can constitute a
+
+31
+00:02:33,110 --> 00:02:36,610
+clinical spacing حتى الشعر حتى الأضفر ممكن يكون
+
+32
+00:02:36,610 --> 00:02:40,310
+عينة لإنه في الآخر could be infected ال skin
+
+33
+00:02:40,310 --> 00:02:45,290
+biopsies organ biopsies all of them can be a
+
+34
+00:02:45,290 --> 00:02:48,710
+spacing عشان هي جسمنا ensemble سباق ال clinical
+
+35
+00:02:48,710 --> 00:02:49,570
+إلى قسمين
+
+36
+00:02:53,190 --> 00:02:56,710
+والصحيحة انا مش مخطط في المحاضرة هذه ان اتكلم عن
+
+37
+00:02:56,710 --> 00:03:00,230
+ال sampling ولا ال samples لان ال samples بحد
+
+38
+00:03:00,230 --> 00:03:05,290
+ذاتها تستغرق مننا محاضرتين او تلاتة عشان نتحدث عن
+
+39
+00:03:05,290 --> 00:03:10,170
+how to collect a good specimen كيف نجمع عينا
+
+40
+00:03:10,170 --> 00:03:13,270
+traceable in a way to avoid contamination with
+
+41
+00:03:13,270 --> 00:03:16,450
+indigenous normal flora بإن الميكروبت باعنا هو
+
+42
+00:03:16,450 --> 00:03:22,620
+اللي نحصل عليهناخد فكرة عن أمثلة عن samples أن
+
+43
+00:03:22,620 --> 00:03:27,600
+body fluids زي blood و urine و cerebrospinal fluid
+
+44
+00:03:27,600 --> 00:03:33,080
+العادة بناخدها بطريقة جيدة و بعدين بنزراحها
+
+45
+00:03:33,080 --> 00:03:36,480
+بنعملها streaming على culture media على سلط
+
+46
+00:03:36,480 --> 00:03:38,940
+culture media في الغالب بعض الأحيان نلجأ ل liquid
+
+47
+00:03:38,940 --> 00:03:44,890
+media لكن mostly على السلط mediaafter incubating
+
+48
+00:03:44,890 --> 00:03:48,530
+the specimen for a certain period of time اللي
+
+49
+00:03:48,530 --> 00:03:52,970
+ممكن تتروح من يوم إلى عدة أيام إلى شهر أو أكتر من
+
+50
+00:03:52,970 --> 00:04:00,930
+ذلك بيظهر عندنا ما يسمى ب ال colony و ال colony هي
+
+51
+00:04:00,930 --> 00:04:06,030
+huge number millions billions of microorganisms
+
+52
+00:04:06,030 --> 00:04:13,790
+جمعت مع بعض نمت تمام فأصبحت ظاهرة ل ال naked eyeمن
+
+53
+00:04:13,790 --> 00:04:18,350
+أجمل الأشياء اللي احنا نمدح فيها ال .. ال .. ال
+
+54
+00:04:18,350 --> 00:04:22,490
+solid media بنقول انه بيوفرلنا إمكانية رؤية
+
+55
+00:04:22,490 --> 00:04:25,290
+المستعمرة ال solid media بينما ال liquid media
+
+56
+00:04:25,290 --> 00:04:28,670
+بتوفرلناهش لإنه النمو فيها بيكون بشكل ايه؟
+
+57
+00:04:28,670 --> 00:04:33,410
+turbidity بيكون بشكل turbidity طيب مال .. مال يعني
+
+58
+00:04:33,410 --> 00:04:38,030
+الممتع ولا مال مفيد في رؤية المستعمرة على ال solid
+
+59
+00:04:38,030 --> 00:04:43,580
+كله على solid plate؟ال solid plate بيوفر لنا
+
+60
+00:04:43,580 --> 00:04:49,620
+مستعمرة، المستعمرة حجمها، لونها، ارتفاعها، شكلها،
+
+61
+00:04:49,620 --> 00:04:54,020
+كلها مرتبطة بنوع البكتيريا، إذا كلمة مرتبطة بنوع
+
+62
+00:04:54,020 --> 00:04:59,000
+البكتيريا إذا هي صفات، والصفات هي اللي احنا
+
+63
+00:04:59,000 --> 00:05:01,880
+استغلناها في عمل التعرف على ال micro organism،
+
+64
+00:05:01,880 --> 00:05:05,120
+مظبوط ولا لأ؟وبالتالي بدنا نعرف ان ال colony
+
+65
+00:05:05,120 --> 00:05:09,860
+morphology، ال size، ال elevation، ال texture أو
+
+66
+00:05:09,860 --> 00:05:14,000
+ال consistency كلها صفات نستخدمها للتعرف على ال
+
+67
+00:05:14,000 --> 00:05:18,360
+microorganism بتعطيك مثال حسابي I would like to
+
+68
+00:05:18,360 --> 00:05:21,440
+describe Staphylococcus aureus colonies on blood
+
+69
+00:05:21,440 --> 00:05:25,720
+agar They are rounded, large, two to four
+
+70
+00:05:25,720 --> 00:05:29,460
+millimeters with golden yellow pigmented colonies.
+
+71
+00:05:30,440 --> 00:05:35,490
+Edges are entireوصفت الان انا اخد بالكوا مستعمرة
+
+72
+00:05:35,490 --> 00:05:42,230
+بلون وحجم وشكل الاستدارة تبع المستعمرة بس ما وصفت
+
+73
+00:05:42,230 --> 00:05:45,810
+انا الوسط او ممكن قولت في سياسة الحديث on blood
+
+74
+00:05:45,810 --> 00:05:51,310
+again يبقى وصف المستعمرة مرتبط بمين؟ بال media
+
+75
+00:05:51,310 --> 00:05:57,350
+اللي نمي عليها ماينفعش اقول description لمستعمرة X
+
+76
+00:05:57,350 --> 00:06:04,400
+الا على وسط Zلش؟ لأن نفس البكتيريا لو زرعناها على
+
+77
+00:06:04,400 --> 00:06:08,960
+وسط تاني قد تختلف شكل مستعمراتها، إذا الوصف مرتبط
+
+78
+00:06:08,960 --> 00:06:15,060
+بمين؟ بنوع الطبق، نوع البكتيريا و نوع الطبق الوصف
+
+79
+00:06:15,060 --> 00:06:19,740
+بتاع المستعمرة مرتبط بالتنين، بالوسط و مرتبط
+
+80
+00:06:19,740 --> 00:06:20,820
+بالكائن نفسه
+
+81
+00:06:23,420 --> 00:06:26,200
+طيب فمن ضمن الأشياء اللي يمكن نستفيد منها خلال
+
+82
+00:06:26,200 --> 00:06:30,120
+فترة الزراعة أنه هل البكتيريا احتلت Oxygen ولا ما
+
+83
+00:06:30,120 --> 00:06:33,600
+احتلت Oxygen؟ يعني هل هي aerobic ولا anaerobic ولا
+
+84
+00:06:33,600 --> 00:06:36,660
+facultative anaerobic ولا micro-aerophilic؟ طبعا
+
+85
+00:06:36,660 --> 00:06:40,120
+مش صفة من صفات، هل احتلت ال room temperature في ال
+
+86
+00:06:40,120 --> 00:06:42,880
+growth ولا ما احتلت ال room temperature؟ انها 37
+
+87
+00:06:42,880 --> 00:06:49,890
+درجة مئوية ولا 42 درجة مئوية؟إذا فيانا صفات أخرى
+
+88
+00:06:49,890 --> 00:06:53,070
+ممكن خلال عملية الزراعة ناخدها زي مين ال oxygen
+
+89
+00:06:53,070 --> 00:06:58,550
+requirement ال temperature ال CO2 requirement هل
+
+90
+00:06:58,550 --> 00:07:02,050
+وضعنا معاها مع ال incubation CO2 و غاز ال CO2 و لا
+
+91
+00:07:02,050 --> 00:07:06,990
+ما وضعناها هذي أيضا أشياء ال pH من ضمن مين من ضمن
+
+92
+00:07:06,990 --> 00:07:10,030
+الطبق نفسه من ضمن الطبق هذا اللي بتكلم عن خارج
+
+93
+00:07:10,030 --> 00:07:13,970
+الطبق احنا الأولانية بنسميها cultural
+
+94
+00:07:13,970 --> 00:07:19,660
+characteristics صفات مزرعيةوبنقسمها لقسمين صفات
+
+95
+00:07:19,660 --> 00:07:25,600
+متعلقة بالمستعبر نفسها تمام وصفات متعلقة بال
+
+96
+00:07:25,600 --> 00:07:30,900
+environment من temperature وحرارة
+
+97
+00:07:30,900 --> 00:07:35,700
+و CO2 ناخد
+
+98
+00:07:35,700 --> 00:07:39,480
+الصورة اللى قدامنا هنا تلخيص وتعبير عما ذكرناه في
+
+99
+00:07:39,480 --> 00:07:43,540
+اللحظة السابقة بس بتفاصيل اكتر عندى ال cup هذه
+
+100
+00:07:43,540 --> 00:07:44,040
+معبرة
+
+101
+00:07:48,990 --> 00:07:52,290
+تم زراعته على طبق زي ما احنا شايفين هنا و بدأت
+
+102
+00:07:52,290 --> 00:07:56,050
+ايه؟ ايه اكتهار عندي مستعمرات و من المستعمرات اللي
+
+103
+00:07:56,050 --> 00:07:59,970
+بنركز عليها لازم تكون well isolated colonies كلمة
+
+104
+00:07:59,970 --> 00:08:03,030
+well isolated colonies يعني منفصلة بشكل جيد بحيث
+
+105
+00:08:03,030 --> 00:08:07,890
+ان اقدر اوصفها لحالها دون ان عن الآخرين و بقدر اخد
+
+106
+00:08:07,890 --> 00:08:12,430
+منها sub culture ايضا لأعمل فحوصات اخرى الآن
+
+107
+00:08:12,430 --> 00:08:16,370
+اللوحة الكبيرة هذه الخضراء اللي ملونة بالأخضر طلع
+
+108
+00:08:16,370 --> 00:08:21,100
+على مستعمرة واحدةمستعمرة واحد لو اتطلعنا عليها من
+
+109
+00:08:21,100 --> 00:08:25,760
+فوق ممكن نجيها منقطة زي ما احنا شايفين هنا ممكن
+
+110
+00:08:25,760 --> 00:08:29,480
+تكون دائرية ممكن تكون filamentous خيطية ممكن تكون
+
+111
+00:08:29,480 --> 00:08:33,700
+irregular ممكن تكون rhizoid عاملة زي اليذور امام
+
+112
+00:08:33,700 --> 00:08:38,720
+او spindle مغزالية لو اتطلعنا عليها ب flat بهذا
+
+113
+00:08:38,720 --> 00:08:43,080
+الشكل يعني هي طبقواطلعنا بهذا المستعمرات على مستوى
+
+114
+00:08:43,080 --> 00:08:47,060
+العين هنشوف مجموعة من ال .. ال .. ال .. الاشياء
+
+115
+00:08:47,060 --> 00:08:51,920
+أخرى زي مثلا هنا flat طبقة رقيقة جدا جدا على ال ..
+
+116
+00:08:51,920 --> 00:08:56,840
+مسطحها على ال .. ال .. الطبق بينما هنا مرتفعة شوية
+
+117
+00:08:56,840 --> 00:09:02,740
+هنا convex هنا ناخدة نصف دائرة تقريبا هنا مقببة
+
+118
+00:09:02,740 --> 00:09:06,650
+أمبونية عاملة زي القبة تمام؟لو جينا طلعنا على
+
+119
+00:09:06,650 --> 00:09:09,710
+الحواف من فوق مرة أخرى طلعنا على المستعمرة بهذا
+
+120
+00:09:09,710 --> 00:09:13,650
+الشكل الحواف تبعتها لما أنا وصفت لستفينيو كوكاس
+
+121
+00:09:13,650 --> 00:09:16,670
+اوريس قلت انها entire edges يبقى بتكلم عن الحواف
+
+122
+00:09:16,670 --> 00:09:21,830
+entire كاملة الاستدارة مش فيها اي تعرج تعرجات
+
+123
+00:09:21,830 --> 00:09:27,430
+undulate مموجة زي ما تشايفين هنا على اللوحة looped
+
+124
+00:09:27,430 --> 00:09:33,830
+مفصصة يعني مقسمة إلى فصوص الجنبها للerosجريمة من
+
+125
+00:09:33,830 --> 00:09:38,950
+مين؟ من لوبيت لكن حجمهم أصغر ما بيسموها مسننة ..
+
+126
+00:09:38,950 --> 00:09:44,830
+مسنن اللي بعدها filamentous و اللي بعدها curled
+
+127
+00:09:44,830 --> 00:09:48,110
+curled معناها مجعنة .. curled من curly .. curly
+
+128
+00:09:48,110 --> 00:09:52,550
+معناها مجعن اللوحة اللي .. او الصورة الأخيرة اللي
+
+129
+00:09:52,550 --> 00:09:56,530
+موجودة في ال .. هذه بتبين أو بتعطينا indication
+
+130
+00:09:56,530 --> 00:09:59,970
+على انه هل البكتينة تهتلت الوسيجين او الـCO2 الى
+
+131
+00:09:59,970 --> 00:10:04,580
+اخر .. دلوقت اخراللوحة اللى أمامنا هذه بتوضح لنا
+
+132
+00:10:04,580 --> 00:10:11,340
+انه سقينة زريعة مستعمرات بدأنا نوصل فيها عرفنا
+
+133
+00:10:11,340 --> 00:10:14,840
+حاجة البكتيريا للاكسجين او ال CO2 او ال
+
+134
+00:10:14,840 --> 00:10:19,680
+temperature من خلالها خطوة تانية و هي الخطوة
+
+135
+00:10:19,680 --> 00:10:23,940
+الرائعة و الأعظم و اللى هنركز عليها نعم هنركز
+
+136
+00:10:23,940 --> 00:10:29,610
+عليها بشكل كبيرالـ Gram stain is the most
+
+137
+00:10:29,610 --> 00:10:33,670
+important technique in conventional microbiology
+
+138
+00:10:33,670 --> 00:10:36,950
+كلمة conventional يعني ال microbiology التقليدية
+
+139
+00:10:36,950 --> 00:10:42,550
+اللي احنا بشتغلها في مختبرات لماذا؟ لأنه بيقسم
+
+140
+00:10:42,550 --> 00:10:46,690
+البكتيريا إلى اتنين ولا اربع انا اليوم احنا اكبرنا
+
+141
+00:10:46,690 --> 00:10:53,210
+شوية من نقسمه لاربع وقسمت عدد كبير من الميكروبات
+
+142
+00:10:53,210 --> 00:10:56,170
+إلى اربع أجزاء أفضل من قسمته إلى جزءين صح؟
+
+143
+00:11:04,720 --> 00:11:08,700
+النص عشرة تلاف العمليات بتتصعب يعني بمعنى اخر لو
+
+144
+00:11:08,700 --> 00:11:14,920
+لاجينا تقنية تقسم البكتيريا الى ستاشر الى اتنين و
+
+145
+00:11:14,920 --> 00:11:18,860
+تلاتين بيكون افضل من ان .. لكن ال gram stem بضربة
+
+146
+00:11:18,860 --> 00:11:23,720
+واحدة بيقسم البكتيريا الى اربع اقصر كيف؟ gram
+
+147
+00:11:23,720 --> 00:11:27,580
+positive و gram negative هي قسمين تحت القسمين
+
+148
+00:11:27,580 --> 00:11:32,260
+الأولينground positive bacilli و ground negative
+
+149
+00:11:32,260 --> 00:11:35,380
+bacilli ground positive cocci و ground negative
+
+150
+00:11:35,380 --> 00:11:38,440
+cocci هاي أربع قسم صراحة اللي بيقولها ال .. ال ..
+
+151
+00:11:38,440 --> 00:11:43,580
+ال .. ال .. ال .. ال spiral organism انسوها الأن
+
+152
+00:11:43,580 --> 00:11:47,820
+خدوا يتكلموا عن مين بس على ال cocci و اللي هم ال
+
+153
+00:11:47,820 --> 00:11:51,300
+bacilli خدوا بالكم القسم اللي أنا ذكرته من الأربع
+
+154
+00:11:51,300 --> 00:11:56,720
+هم العناوين لأي إنسان بيعمل في مجال ال
+
+155
+00:11:56,720 --> 00:12:02,060
+microbiologyأول عنوان بدنا نعرف عنه البكتيريا لازم
+
+156
+00:12:02,060 --> 00:12:06,900
+انها gram stain و morphology او الكلاش عزلنا
+
+157
+00:12:06,900 --> 00:12:09,780
+بكتيريا من X مريض .. من مريض و عملنا لها gram
+
+158
+00:12:09,780 --> 00:12:14,760
+stain طلعت gram negative coccyte الان اللي ..
+
+159
+00:12:14,760 --> 00:12:19,940
+العامل اللي بيحدد اختيار ده الفحوصات هو العنوان
+
+160
+00:12:19,940 --> 00:12:24,020
+الكبيرإذا كانت جرام نيجاتيف كوكساي أول فحص بعمل
+
+161
+00:12:24,020 --> 00:12:28,380
+Oxidase وبعدين بعمل فحصات زي glucose oxidation
+
+162
+00:12:28,380 --> 00:12:34,470
+testإذا كانت gram بصتف بسلعي بعمل كتاليز بعملش
+
+163
+00:12:34,470 --> 00:12:38,890
+وكتاليز إذا مين حدد الفحصات التالية الانواع
+
+164
+00:12:38,890 --> 00:12:40,790
+الرئيسي؟ مين الانواع الرئيسي منجبناه؟ من ال
+
+165
+00:12:40,790 --> 00:12:46,210
+Granstein امام؟ إذا بدون أدنى شك ما زال ال
+
+166
+00:12:46,210 --> 00:12:50,230
+Granstein التقنية الأساسية اللي بنستخدمها في
+
+167
+00:12:50,230 --> 00:12:53,230
+التعرف على ال micro organs وبالتالي أنا بقول ..
+
+168
+00:12:53,230 --> 00:12:56,190
+أنا قولت للطلاب بقولكم المرة .. إيه ال Quran؟إذا
+
+169
+00:12:56,190 --> 00:12:58,930
+انتوا الأحد دلوقت شاعرين ان انتوا مش جادرين
+
+170
+00:12:58,930 --> 00:13:02,290
+تتميزوا بين الـ ground positive و ground negative
+
+171
+00:13:02,290 --> 00:13:06,330
+لأ اطلبوا من المدرسات المعمل انهم يعملوا لكوا
+
+172
+00:13:06,330 --> 00:13:09,530
+review حتى تتأكدوا انكم بالفعل بتعرفوا ال ground
+
+173
+00:13:09,530 --> 00:13:11,130
+positive من ال ground negative بتعرفوا ال بس الله
+
+174
+00:13:11,130 --> 00:13:15,810
+من الكوكساي بتعرفوا ال arrangements تمام؟ مهم جدا
+
+175
+00:13:15,810 --> 00:13:19,850
+المعلومة، فضلوا الحين لما نستخدم ال ground .. ال
+
+176
+00:13:19,850 --> 00:13:22,670
+ground negative تقسم عن ال ground positive و
+
+177
+00:13:22,670 --> 00:13:23,230
+ground negative؟
+
+178
+00:13:26,010 --> 00:13:29,890
+الموضوع استثناء استثناء هذا استثناء احنا ماجداش
+
+179
+00:13:29,890 --> 00:13:32,690
+نشوف اللي ماجداش نصفه لما انا بستخدم ال ground
+
+180
+00:13:32,690 --> 00:13:35,430
+stain يا مابتنصف ال ground بصي يا ماجداش نجد فيه
+
+181
+00:13:35,430 --> 00:13:39,390
+يا مابشوفهاش، مظبوط؟ فبعرفهاش، مادام مشوفتهاش انا
+
+182
+00:13:39,390 --> 00:13:42,830
+في جامعة بعرفهاش فبتطبق عليها تقنية تانية، حيمول
+
+183
+00:13:42,830 --> 00:13:46,050
+معانا كمان شوية بعض التقنيات الموظفة أو اللي
+
+184
+00:13:46,050 --> 00:13:49,270
+بنوظفها لما نلاجي مسائل من النوع هذا، بكتيريا
+
+185
+00:13:49,270 --> 00:13:53,410
+ماجداش ننميها، بكتيريا بطيقة نمو، هنشوف كيف نلاجي
+
+186
+00:13:53,410 --> 00:13:58,630
+حل معاهاطيب قبل ما اطلع من ال grammar stain انا
+
+187
+00:13:58,630 --> 00:14:02,090
+بدي احكي معلومة مهمة جدا ان ال grammar stain ممكن
+
+188
+00:14:02,090 --> 00:14:09,280
+وضفه مرتين فاكرين العينة؟هذه العينة، اعتبروا هذه
+
+189
+00:14:09,280 --> 00:14:13,700
+العينة مش urine، اعتبروها CSF، ليش CSF؟ لأن هو بدأ
+
+190
+00:14:13,700 --> 00:14:17,840
+.. أعطيكوا مؤشر خطورة، أكيد عينة ال urine أقل
+
+191
+00:14:17,840 --> 00:14:22,040
+الخطورة من عينة مين؟ ال CSF ال CSF ال infection في
+
+192
+00:14:22,040 --> 00:14:26,740
+.. في منطقة حساسة جدا و أي خلية عصبية بتموت لم يكن
+
+193
+00:14:26,740 --> 00:14:33,420
+هناك regeneration إلافتعتبر يعني عينة احنا بنسميها
+
+194
+00:14:33,420 --> 00:14:38,760
+emergency specimen، بنوقف كل الشغل و بنشتغل CSF طب
+
+195
+00:14:38,760 --> 00:14:41,720
+أنا جالبا بتكلم عن مزرعة، مزرعة بيتها 24 ساعة و
+
+196
+00:14:41,720 --> 00:14:44,220
+بيتها كمان في فترة 24 ساعة و sensitivity كمان 24
+
+197
+00:14:44,220 --> 00:14:48,440
+ساعة يعني 72 ساعة، هل من المنطق انه نترك المريض
+
+198
+00:14:48,440 --> 00:14:52,300
+بدون علاج لفترة 78 ساعة و أنا بتكلم عن عينة CSF
+
+199
+00:14:54,360 --> 00:14:57,520
+يبقى انا ممكن استخدم او وضف ال gram stain مباشرة
+
+200
+00:14:57,520 --> 00:15:01,980
+على العينة انا اعمل centrifigation للعينة و اخد ال
+
+201
+00:15:01,980 --> 00:15:05,960
+sediment و اعمل ال gram stain من العينة مباشرة من
+
+202
+00:15:05,960 --> 00:15:10,820
+العينة مباشرةإذا ولدت فيها بكتيريا، معناه حق أنه
+
+203
+00:15:10,820 --> 00:15:13,540
+في بكتيريا ال infection، يبقى الطبيب بيعرف أنه في
+
+204
+00:15:13,540 --> 00:15:16,640
+بكتيريا ال infection، جديد الفترة الزمنية اللي
+
+205
+00:15:16,640 --> 00:15:21,680
+أخدتني و أخدتها حتى أصدر نتيجة تقريبا نصف ساعة،
+
+206
+00:15:21,680 --> 00:15:25,480
+فخلال نصف ساعة أنا باعطي للطبيب معلومات أنه في
+
+207
+00:15:25,480 --> 00:15:28,000
+بكتيريا أو مافيش بكتيريا، positive أو negative،
+
+208
+00:15:28,000 --> 00:15:30,520
+وإذا كان في بكتيريا، هل هي gram positive ولا gram
+
+209
+00:15:30,520 --> 00:15:33,680
+negative، وإذا كان positive أو negative، هل هي
+
+210
+00:15:33,680 --> 00:15:37,700
+بصلة ولا oxide، هذا بيساعد الطبيب على أنه يختار
+
+211
+00:15:38,200 --> 00:15:41,500
+more selective therapy لحين ظهور نتيجة علمية
+
+212
+00:15:41,500 --> 00:15:44,940
+للمزارع، إلى ال gramsteria استخدمت وانا مرتين، مرة
+
+213
+00:15:44,940 --> 00:15:49,640
+مباشرة مع ال specimen ومرة في التعرف على البكتيريا
+
+214
+00:15:49,640 --> 00:15:51,460
+بعد زراعتها من المزارع
+
+215
+00:15:54,300 --> 00:15:57,660
+هذه الصورة اللي قدامنا بتوضح أن بعض الأشكال و
+
+216
+00:15:57,660 --> 00:16:01,360
+الألوان لونان مشهورين اللي هو ال pink و ال violet
+
+217
+00:16:01,360 --> 00:16:03,940
+ال violet لل gram positive و ال pink لل gram
+
+218
+00:16:03,940 --> 00:16:24,280
+negative
+
+219
+00:16:26,900 --> 00:16:32,140
+اللون اللي بتاعة هدا؟ ليش هدا؟ blue مثلا يعني؟ اه
+
+220
+00:16:32,140 --> 00:16:34,220
+لو وامكن عندي انا ال pink عنكم انتوا هتصبولا مش
+
+221
+00:16:34,220 --> 00:16:40,110
+مبينبس عموما ال .. ال .. ال .. ال .. فوق هنا
+
+222
+00:16:40,110 --> 00:16:43,450
+ground positive و فيه عندي بسلاي و فيه دبلو كوكاي
+
+223
+00:16:43,450 --> 00:16:48,970
+و فيه عندي chains تمام سلاسل او ستربتو فيه دبلو و
+
+224
+00:16:48,970 --> 00:16:52,890
+فيه عندي هنا clusters بينما هنا فيه دبلو كوكساي
+
+225
+00:16:52,890 --> 00:16:57,110
+ground negative هنا single بسلاي او بالاخر ممكن
+
+226
+00:16:57,110 --> 00:17:01,170
+يسموها cocoa بسلاي لأنها متفقنة و عنها قصيرة هنا
+
+227
+00:17:01,170 --> 00:17:04,690
+فيه عندنا بسلاي اشي بكبسول و اشي بدون كبسول و فيه
+
+228
+00:17:04,690 --> 00:17:10,360
+عندنا هنا longلكن البسلاي أشكال و ألوان مختلفة و
+
+229
+00:17:10,360 --> 00:17:15,440
+اللي حابب أنه يعني يشوف تنوع الميكروبات يجيب عينة
+
+230
+00:17:15,440 --> 00:17:19,380
+sewage مجاري و يحطها و يعمل منها ground stain او
+
+231
+00:17:19,380 --> 00:17:23,200
+يطلع عليها حتى و يتموت و يشوف الأشكال هيشوف spiral
+
+232
+00:17:23,200 --> 00:17:28,260
+و هيشوف coaxial و هيشوف ممكن مربعات كمان يعني rare
+
+233
+00:17:28,260 --> 00:17:33,250
+bacteriaالصورة اللى قدامنا هنا بتوضح انها يمكن
+
+234
+00:17:33,250 --> 00:17:38,030
+بصورة كمان حقيقية هذه تحت ال microscope وبالتالي
+
+235
+00:17:38,030 --> 00:17:41,270
+الآن بدأت المشكلة بدأت المشكلة الصورة هنا ال gram
+
+236
+00:17:41,270 --> 00:17:46,090
+-bacilli gram-booster cocci هنا gram-diplicocci
+
+237
+00:17:46,090 --> 00:17:49,790
+هنا filamentous خيطية اللي أخر كده متشابكة مع
+
+238
+00:17:49,790 --> 00:17:57,250
+بعضها البكتيريا هذا سموها لكن في الآخر اللون تبعها
+
+239
+00:17:57,250 --> 00:18:02,560
+violet ومخدة اللي هو ال gram-booster reactionطيب
+
+240
+00:18:02,560 --> 00:18:06,620
+زرعنا العينة طلع من مستعمرات عملنا gram stain و
+
+241
+00:18:06,620 --> 00:18:08,920
+عرفنا ايش هو ال gram stain تبع البكتيريا what is
+
+242
+00:18:08,920 --> 00:18:14,560
+the next step؟ biochemical identification و هذه
+
+243
+00:18:14,560 --> 00:18:16,960
+الخطوة اللي هتبدأوا فيها في المعمل اعتقد الأسبوع
+
+244
+00:18:16,960 --> 00:18:20,720
+هذا اللي هو ال biochemical tests ال biochemical
+
+245
+00:18:20,720 --> 00:18:24,280
+tests انا سألت المثات اللي في المعمل و قالوا ان
+
+246
+00:18:24,280 --> 00:18:28,740
+الأسبوع هذا هنبدأ في ال biochemical reactionsمن
+
+247
+00:18:28,740 --> 00:18:31,060
+ضمن ال biochemical reaction مجموعة ضخمة من
+
+248
+00:18:31,060 --> 00:18:35,300
+الفحوصات اللي هم زي ال indole و ال methyl red و
+
+249
+00:18:35,300 --> 00:18:39,180
+الbocostroskara و ال citrate مروا معاها فحوصات على
+
+250
+00:18:39,180 --> 00:18:42,740
+فكرة قبل هيك احنا في النظرية لما اتكلمنا عن
+
+251
+00:18:42,740 --> 00:18:44,460
+البكتيري الميتابوليزم ده بتذكر
+
+252
+00:18:48,060 --> 00:18:51,020
+لما بنقول positive و بنقول negative احنا اتفقنا في
+
+253
+00:18:51,020 --> 00:18:55,340
+ال microbiology لا يوجد دا هنا قيمة صفرية يعني حد
+
+254
+00:18:55,340 --> 00:18:58,200
+ما .. مالاش معنى كل إشي له معنى سواء negative أو
+
+255
+00:18:58,200 --> 00:19:01,340
+ال positive إيه له معنى؟ يعني مثلا بنقول الشريشة
+
+256
+00:19:01,340 --> 00:19:05,000
+كلها ان دول positive بنقول ال enterobacter ان دول
+
+257
+00:19:05,000 --> 00:19:08,180
+negative أنا بالنسبة ليه ال negative و ال positive
+
+258
+00:19:08,180 --> 00:19:13,280
+هم صفة غير موجودة و موجودة زي ما ورّح اقولكوا
+
+259
+00:19:13,280 --> 00:19:17,280
+بيلبس نظارات او ما .. مابيلبس نظارات يا صفة موجودة
+
+260
+00:19:17,990 --> 00:19:24,150
+فبتالي وزنها واحد اتنين ووزنهم بالنسبالنا واحد طيب
+
+261
+00:19:24,150 --> 00:19:28,690
+انا بده اعقد الأمور عشان اسهلها في نفس الوقت عندي
+
+262
+00:19:28,690 --> 00:19:35,510
+200 بكتيريا 200 بكتيريا احفظوا 200 اسم 200 اسم 200
+
+263
+00:19:35,510 --> 00:19:39,070
+بكتيريا يعني 200 اسم بكتيريا مختلفة عشان اميزهم مع
+
+264
+00:19:39,070 --> 00:19:45,450
+بعض بده اعملهم كل واحدة عشرين فحصيعني 200 ضرب 20
+
+265
+00:19:45,450 --> 00:19:48,550
+اذا كان positive و negative يطلع معاه رقم ضخم
+
+266
+00:19:48,550 --> 00:19:53,490
+هتصحي و تنامي و انت تقولي positive ولا negative؟
+
+267
+00:19:53,490 --> 00:19:56,210
+negative ولا positive؟ و اليوم .. و اليوم ممكن
+
+268
+00:19:56,210 --> 00:19:59,170
+تحفظيهم يعني في ناس عندهم photo memory و تانية و
+
+269
+00:19:59,170 --> 00:20:03,530
+تكوني نسيهم لذلك ليس من المنطقي انه نحفظ كل
+
+270
+00:20:03,530 --> 00:20:09,390
+الفحوصات لل 220 اللي بناهم كان هذا major
+
+271
+00:20:09,390 --> 00:20:15,240
+differentiation testهذا المين للطلبة و العاملين في
+
+272
+00:20:15,240 --> 00:20:21,560
+المجال لما بنكبر شوية بنبدأ نتعلم بال softwares،
+
+273
+00:20:21,560 --> 00:20:26,600
+بندخل البيانات، بندخل البيانات اللي هم ال 200
+
+274
+00:20:26,600 --> 00:20:31,900
+بكتيريا و ال 20 فحص و نتائجهمتمام؟ و لما بنعزل
+
+275
+00:20:31,900 --> 00:20:35,300
+ميكروبي جديد احنا نتعرف .. نعمله فحصات ايش بيطلع
+
+276
+00:20:35,300 --> 00:20:37,780
+الاتاجوب ندخلهم على الكمبيوتر بيقارنهم بقاعة
+
+277
+00:20:37,780 --> 00:20:41,340
+البيانات بيطلعنا بيقولنا you're bacteria is هذا
+
+278
+00:20:41,340 --> 00:20:45,580
+لأنه احنا فيكم نحفظ بعض الفحصات اللي بيسميناها
+
+279
+00:20:45,580 --> 00:20:51,320
+major differentiation test يعني لما اقول Shigella
+
+280
+00:20:51,320 --> 00:20:55,120
+non motile و Salmonella motile هذا يعني ال
+
+281
+00:20:55,120 --> 00:20:57,280
+differentiation test بتاع اللي لازم احنا نتعرف
+
+282
+00:20:57,280 --> 00:21:01,180
+عليهو تصورة ان ال biochemical reaction مهم جدا في
+
+283
+00:21:01,180 --> 00:21:03,760
+عملية دي اللي هو التعرف و هتعرف عليها شغالة بشكل
+
+284
+00:21:03,760 --> 00:21:06,800
+جيد فين دي حاسبة immunological methods
+
+285
+00:21:06,800 --> 00:21:10,200
+immunological methods اللي هي سطر التالت
+
+286
+00:21:10,200 --> 00:21:15,240
+immunological methods وهي عبارة عن antigen
+
+287
+00:21:15,240 --> 00:21:18,960
+detection او antibody detection تركزوا معاه في
+
+288
+00:21:18,960 --> 00:21:22,900
+البسيطية دي لأنها كتير مهمة هناك فرق كبير بين ال
+
+289
+00:21:22,900 --> 00:21:28,310
+antigen و بين ال antibody detectionالان ال antigen
+
+290
+00:21:28,310 --> 00:21:32,470
+هو جزء من مين؟ من البكتيريا على اسم المثال ال
+
+291
+00:21:32,470 --> 00:21:37,630
+antibody هو ال response تبعنا للبكتيريا طيب طلعوا
+
+292
+00:21:37,630 --> 00:21:42,390
+على الحالة التلاتة البكتيريا لو مسكنها مباشرة في
+
+293
+00:21:42,390 --> 00:21:46,550
+العينة نفس البكتيريا بال culture صح ولا لأ؟ او بال
+
+294
+00:21:46,550 --> 00:21:50,950
+staining يبقى احنا قبضنا على الميزورين المتلبس
+
+295
+00:21:50,950 --> 00:21:57,280
+بذاته بذاته بدون اي اشاراتلكن الانتجين هو جزء من
+
+296
+00:21:57,280 --> 00:22:04,440
+البكتيريا و ليس البكتيريا الان الأسوأ من الجزء هو
+
+297
+00:22:04,440 --> 00:22:13,020
+الأثر واحد ارتكب جريمة قبل سنة وصار يريد في نفس
+
+298
+00:22:13,020 --> 00:22:19,300
+المكان اليوم كويس؟ إت المحقق و أخد بصمات من ضمن
+
+299
+00:22:19,300 --> 00:22:25,820
+البصمات أخد بصمات من؟ اللي قبل سنةفالان بيجيبوه
+
+300
+00:22:25,820 --> 00:22:29,380
+انت قتلت X اللي هو الجديد بيقولوا لا يا عم انا ما
+
+301
+00:22:29,380 --> 00:22:35,700
+قتلتش انا قتلت Y قبل سنة مش اليوم يبقى وجود أثر
+
+302
+00:22:35,700 --> 00:22:41,040
+للميكروب بشكل Antibody لا يعني وجود الميكروب اللي
+
+303
+00:22:41,040 --> 00:22:44,440
+قد يكون موجود في السابق وانا عارفين ان ال Antibody
+
+304
+00:22:44,440 --> 00:22:52,130
+أصلا انتج كاستجابة لل Antigen عشانيظل فيه أنه نوع
+
+305
+00:22:52,130 --> 00:22:56,470
+من المناعة ضد هذا المجرم، إذا ليس الكشف عن ال
+
+306
+00:22:56,470 --> 00:23:00,570
+antigen كالكشف عن مين؟ عن ال antibody أو عن
+
+307
+00:23:00,570 --> 00:23:04,430
+البكتيريا، مين أقوى حاجة أن نكشف البكتيريا؟ اللي
+
+308
+00:23:04,430 --> 00:23:09,730
+بعديها ال antigen و آخر حاجة مين؟ أنا لا .. دائما
+
+309
+00:23:09,730 --> 00:23:14,490
+و أبدا بقول لا أستطيع أن أقارن بين شغلتين، بين
+
+310
+00:23:14,490 --> 00:23:18,470
+فحصين موجودين و أقول هذا أحسن من هذاليش؟ لأنه كون
+
+311
+00:23:18,470 --> 00:23:22,130
+ال trend موجودين يبقى هذا له استخدامه و هذا له
+
+312
+00:23:22,130 --> 00:23:26,250
+استخدامه مش دايما بقدر أجي ال antigen فممكن أبحث
+
+313
+00:23:26,250 --> 00:23:29,890
+عن ال antibody في بعض الأحيان لكن بدا أكون فاهم
+
+314
+00:23:29,890 --> 00:23:34,390
+أنه قيمته لا تعني اللي هو وجود ال antigen أو تعني
+
+315
+00:23:34,390 --> 00:23:38,190
+وجود ال micro organism و عندي مجموعة احتمالات
+
+316
+00:23:38,190 --> 00:23:43,090
+هتكلم عنها لاحقا ان شاء الله اذا biochemical test
+
+317
+00:23:43,090 --> 00:23:51,060
+هتناخد قصةخدنا عينة من CSF عملنا لها streaking على
+
+318
+00:23:51,060 --> 00:23:56,460
+أكتر من وسط و طلعنا بكتيريا الواصف تبع المستعمرات
+
+319
+00:23:56,460 --> 00:23:58,620
+golden yellow pigmented فاكرين ال golden yellow
+
+320
+00:23:58,620 --> 00:24:03,020
+pigmented؟ على البلد أجار و عاملة beta hemolysis و
+
+321
+00:24:03,020 --> 00:24:06,780
+entire edge عملنا gram stain لجيناها gram postif
+
+322
+00:24:06,780 --> 00:24:10,440
+cocci in clusters عملتلها biochemical test كتاليز
+
+323
+00:24:10,440 --> 00:24:13,240
+test طلعت postif عملتلها coagulase طلعت postif
+
+324
+00:24:13,240 --> 00:24:19,020
+البكتيريا الأن استنفي ل focus areasعرفتها مش على
+
+325
+00:24:19,020 --> 00:24:26,420
+الوصف، وصف Gramstein و biochemical testبعض الأحيان
+
+326
+00:24:26,420 --> 00:24:31,860
+لا يكفي قصة جديدة عيد سطول زرعناه طلعت gram
+
+327
+00:24:31,860 --> 00:24:34,820
+negative بسلاي على ماكن كلاكتوز fermenter شكلها
+
+328
+00:24:34,820 --> 00:24:38,920
+pinkish colonies عملتلها biochemical test ان دول
+
+329
+00:24:38,920 --> 00:24:43,240
+test بُستف و بوكرسبلسكاور اه لأ احنا بيقولنا MR
+
+330
+00:24:43,240 --> 00:24:46,520
+بُستف و بوكرسبلسكاور negative citrate negative
+
+331
+00:24:46,520 --> 00:24:49,640
+يعني بُستف بُستف negative negative إشريش أكل يعني
+
+332
+00:24:49,640 --> 00:24:54,460
+طب وش و سوا كنا أنا في السطول إشريش أكل صح ولا لأ؟
+
+333
+00:24:54,580 --> 00:24:58,820
+يبقى كيف بدي أميز E.coli ممرضة وعن ال E.coli
+
+334
+00:24:58,820 --> 00:25:02,280
+الممرضة بدي أعمل serology الان بدي أشوف هل عندها
+
+335
+00:25:02,280 --> 00:25:07,000
+O157 الانتجين تبعها موجود ده ممرضة ولا لأ يبقى بعض
+
+336
+00:25:07,000 --> 00:25:09,400
+الأحيان اللي هيكفي ال biochemical identification
+
+337
+00:25:09,400 --> 00:25:14,720
+ونضطر لمين الآن لل serological identification طيب
+
+338
+00:25:15,470 --> 00:25:18,630
+زميلتكن تنبهت لقضية جالت الدكتورة ما هو مش كل ..
+
+339
+00:25:18,630 --> 00:25:22,290
+مش كل البكتيريا بتنسبق لـ gram stain و gram
+
+340
+00:25:22,290 --> 00:25:25,430
+positive و gram negative و قولت أنا كمان إضافة مش
+
+341
+00:25:25,430 --> 00:25:28,950
+كل البكتيريا أصلا نقدر ننميها و مش كل البكتيريا
+
+342
+00:25:28,950 --> 00:25:33,830
+بتنمو بشكل سريع زي ما كنا 24 ساعة عشان نقدر نحصل
+
+343
+00:25:33,830 --> 00:25:40,230
+على نتيجة تخلال 72 ساعة فهل هناك طرق لل rapid
+
+344
+00:25:40,230 --> 00:25:47,170
+diagnosis بدون مزرعة؟ نعمواحد بدنا نعرف في أي
+
+345
+00:25:47,170 --> 00:25:50,990
+حالات من الأول culture أو certain human pathogenes
+
+346
+00:25:50,990 --> 00:25:53,490
+including the causative agent of tuberculosis اللي
+
+347
+00:25:53,490 --> 00:25:56,070
+هي ال micro bacterium tuberculosis ال lime disease
+
+348
+00:25:56,070 --> 00:25:58,410
+اللي هي ال borrelia و ال syphilis اللي هي
+
+349
+00:25:58,410 --> 00:26:02,070
+tryponema pallidium either cannot be isolated إما
+
+350
+00:26:02,070 --> 00:26:08,790
+إنها مابنجرش نفسها أساسا أو إنه slow growth طب هذه
+
+351
+00:26:08,790 --> 00:26:14,550
+مشكلةالتربونية بلدهم لحد الآن لم تعزل على
+
+352
+00:26:14,550 --> 00:26:18,230
+artificial medium الميكروباكتيروم تبركلوسس بتقعد
+
+353
+00:26:18,230 --> 00:26:24,110
+ست تسابيع البوريليا ممكن تقعد أسبوع كلهم فيهم
+
+354
+00:26:24,110 --> 00:26:29,910
+مشكلة يا بطيئة يا ما تنموش فبالتالي
+
+355
+00:26:29,910 --> 00:26:32,670
+ال successful isolation في الفترة التانية غالبا
+
+356
+00:26:32,670 --> 00:26:36,570
+بيكون اما سلوك وبعض الأحيان impossible مستحيل
+
+357
+00:26:38,160 --> 00:26:40,700
+بالحالة direct detection of bacteria without
+
+358
+00:26:40,700 --> 00:26:45,380
+culture is possible in some cases اعطيكم مثال لو
+
+359
+00:26:45,380 --> 00:26:51,240
+احنا أخدنا عين تسبيوتام احنا بيقولنا المريض ال
+
+360
+00:26:51,240 --> 00:26:54,140
+tuberculosis هو يبقى او غالبا palmonary
+
+361
+00:26:54,140 --> 00:26:58,200
+tuberculosis فبيكون فيه اللي هو ال .. ال .. ال ..
+
+362
+00:26:58,200 --> 00:26:58,580
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+363
+00:26:58,580 --> 00:26:58,780
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+364
+00:26:58,780 --> 00:26:59,060
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+365
+00:26:59,060 --> 00:26:59,260
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+366
+00:26:59,260 --> 00:26:59,580
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+367
+00:26:59,580 --> 00:26:59,600
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+368
+00:26:59,600 --> 00:27:00,100
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+369
+00:27:00,100 --> 00:27:00,220
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+370
+00:27:00,220 --> 00:27:04,100
+ال .. ال .. ال .. ال ..مش درستوا انتوا acid fast
+
+371
+00:27:04,100 --> 00:27:07,220
+stain؟ طب لو عملنا ال dispute ال acid fast stain
+
+372
+00:27:07,220 --> 00:27:11,340
+مش خلال نصف ساعة بنقدر نشوف برضه ال acid fast بس
+
+373
+00:27:11,340 --> 00:27:14,480
+ال lie of presence or absence بنقدر من درج بنقدر
+
+374
+00:27:14,480 --> 00:27:17,200
+هذا rapid .. rapid approach ولا مش rapid approach؟
+
+375
+00:27:17,200 --> 00:27:20,980
+Rapid approach خدوا بالكوا نفس ال rapid approach
+
+376
+00:27:20,980 --> 00:27:23,780
+اللي هو ال staining بال acid fast في كمان staining
+
+377
+00:27:23,780 --> 00:27:27,680
+تاني ما أذكر معاكوا ولا درستوا في المعمل اسمه
+
+378
+00:27:27,680 --> 00:27:31,490
+fluorescent staining fluorescent stainو ماذا انا
+
+379
+00:27:31,490 --> 00:27:33,550
+اقصد ب fluorescence studying لإنه كتير concept مهم
+
+380
+00:27:33,550 --> 00:27:36,030
+اسمه مش بدكورة معانا بس انا متأكد هتمور معانا
+
+381
+00:27:36,030 --> 00:27:39,910
+somewhere فيرد ان تبقى لها كويس و انا هشرع على
+
+382
+00:27:39,910 --> 00:27:44,030
+الصبورة بعض ال .. ال .. الاشياء المتعلقة بال
+
+383
+00:27:44,030 --> 00:27:48,730
+fluorescence عشان تكون الصورة أوضح ان شاء الله
+
+384
+00:27:48,730 --> 00:27:53,790
+بالنسبة لنا هتبقى اقولنا انه مادة ال fluorescence
+
+385
+00:27:53,790 --> 00:27:57,910
+هي substance لو ضربناها بال UV كلمة ضربناها يعني
+
+386
+00:27:57,910 --> 00:28:02,050
+اذا زرق عليها شعرit will absorb الآن ال absorption
+
+387
+00:28:02,050 --> 00:28:05,450
+سباق ال UV بيعمل excitation لل fluorescent
+
+388
+00:28:05,450 --> 00:28:11,610
+substance وبيعمل emitting لvisible light وكلمة
+
+389
+00:28:11,610 --> 00:28:14,890
+visible light هي ضوء مرقى والضوء المرقى هنا غالبا
+
+390
+00:28:14,890 --> 00:28:19,050
+بيكون colored colored يعني أما لون أصفر أو أخضر أو
+
+391
+00:28:19,050 --> 00:28:22,130
+أزرق أو أحمر يعني بيختلف من حسب نوع مين ال
+
+392
+00:28:22,130 --> 00:28:28,430
+fluorescent substance لو علمتمأن هناك صبغة بترتبط
+
+393
+00:28:28,430 --> 00:28:31,970
+specifically بال mycolic acid ايش هو ال mycolic
+
+394
+00:28:31,970 --> 00:28:39,690
+acid؟ ال mycolic acid هو ال واكسي لبت اللي موجود
+
+395
+00:28:39,690 --> 00:28:44,630
+في ال mycobacterium تبركيلووسس اسم هذه البكتيريا
+
+396
+00:28:44,630 --> 00:28:53,030
+هي ال mycolic acid اسم
+
+397
+00:28:53,030 --> 00:28:58,220
+المادة Rodamineو في مادة أخرى اسمها Oramine
+
+398
+00:28:58,220 --> 00:29:01,760
+Oramine
+
+399
+00:29:01,760 --> 00:29:08,020
+O و Rodamine أين منهما أو كلهما بيتطابط
+
+400
+00:29:08,020 --> 00:29:13,580
+Specifically مع ميجولك أسد لو صبغنا عيلة شريحة
+
+401
+00:29:13,580 --> 00:29:20,140
+فيها fixed smear من اللي هو ال .. ال .. ال .. ال
+
+402
+00:29:20,140 --> 00:29:20,200
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+403
+00:29:20,200 --> 00:29:20,360
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+404
+00:29:20,360 --> 00:29:20,640
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+405
+00:29:20,640 --> 00:29:21,260
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+406
+00:29:21,260 --> 00:29:22,140
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+407
+00:29:22,140 --> 00:29:22,900
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+408
+00:29:22,900 --> 00:29:22,920
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+409
+00:29:26,030 --> 00:29:28,430
+حايل .. حايل .. حايل .. حايل سبقها وكونوا
+
+410
+00:29:28,430 --> 00:29:31,210
+specifically في الميكوليك أسد يبجلان يسبق
+
+411
+00:29:31,210 --> 00:29:35,170
+البكتيريا الأخرى فقط من؟ الميكو بكتيريا بعدها نعمل
+
+412
+00:29:35,170 --> 00:29:39,510
+عملية .. عملية غسيل و بنطفي في مادة اسمها بُتاسيم
+
+413
+00:29:39,510 --> 00:29:42,370
+بالمنجليات بنخفف من ال fluorescence اللي تبقى
+
+414
+00:29:42,370 --> 00:29:45,750
+الصبغة و بتنطلع تحت الميكروسكوب المرة هذه بمعناه
+
+415
+00:29:45,750 --> 00:29:50,130
+أسهل detection هتشوفوه في حياتكم هو الميكو بكتيريا
+
+416
+00:29:50,130 --> 00:29:53,310
+متبركوليستيكيا على ال high power تعرف ال high
+
+417
+00:29:53,310 --> 00:29:53,690
+power؟
+
+418
+00:29:56,700 --> 00:30:00,340
+مش ال oil، ال oil immersion مية، مظبوط؟ يعني على
+
+419
+00:30:00,340 --> 00:30:04,120
+أربعين اكس ممكن نعمل detection لمين لاللي هو ال
+
+420
+00:30:04,120 --> 00:30:07,860
+micro bacterium tuberculosis، إذا هي تقنية برضه
+
+421
+00:30:07,860 --> 00:30:13,540
+بال staining و rapid و rapid و سريعة و easyو للأسف
+
+422
+00:30:13,540 --> 00:30:16,880
+لحد الآن لم تطبقوا .. يمكن السنة هذه تطبقوها في ..
+
+423
+00:30:16,880 --> 00:30:20,480
+في أحد العيادات في قطاع غزة لكن احنا كجامعة في
+
+424
+00:30:20,480 --> 00:30:24,300
+المختبر الصحيح عملناها يمكن قبل خمساش سنة و ورناهم
+
+425
+00:30:24,300 --> 00:30:27,780
+نتعيش هنا و نالهم هاي الصبغة اتفضلوا هدية gift بس
+
+426
+00:30:27,780 --> 00:30:32,280
+انتوا عملوا الفحوصات لكن تعقيدات الإدارية غالبا
+
+427
+00:30:32,280 --> 00:30:38,820
+بتكون معينةاحنا نسميها تعقيدات إدارية، عشانك مش
+
+428
+00:30:38,820 --> 00:30:41,980
+جادين نفسرها لإيش يعني، نفسيات الناس، يعني كيف
+
+429
+00:30:41,980 --> 00:30:46,140
+تشتغل مبنها طيب،
+
+430
+00:30:46,140 --> 00:30:51,760
+فين دي هنا أحد الطرق للكشف عن الانتيجنات؟ يمكن هنا
+
+431
+00:30:51,760 --> 00:30:56,240
+الأطفال أكثر فئة بتاعي من التنسوليتس أو إتهاب
+
+432
+00:30:56,240 --> 00:31:01,940
+الحلقوالمسبب الأكبر .. المسبب الأكبر لانتهابات
+
+433
+00:31:01,940 --> 00:31:06,220
+الحلق هو ال .. ال bacterial cause هو ال .. the
+
+434
+00:31:06,220 --> 00:31:10,660
+streptococcus biogenes طيب أنا أقعد تلت أيام
+
+435
+00:31:10,660 --> 00:31:14,720
+culture .. تلت أيام culture كاملة من ألفها إلي آخر
+
+436
+00:31:14,720 --> 00:31:19,780
+تقريبا هذا ال minimum 72 ساعة و أترك المريض بدون
+
+437
+00:31:19,780 --> 00:31:24,240
+علاج طول الفترة هذه الصحيح بتكون الأمر مزعجفبناخد
+
+438
+00:31:24,240 --> 00:31:29,060
+عينة من المريض مسحة ب cotton swab شايفين الإشارة
+
+439
+00:31:29,060 --> 00:31:32,560
+اللي هنا عبارة
+
+440
+00:31:32,560 --> 00:31:37,700
+عن عوض رفيق على طرفه قطعة ثميقة من ال cotton
+
+441
+00:31:37,700 --> 00:31:44,320
+ملفوفة بطريقة صلبة بنعمل illumination جيد للفم و
+
+442
+00:31:44,320 --> 00:31:47,960
+بنضغط على اللسان بخافض لللسان يسموه tongue
+
+443
+00:31:47,960 --> 00:31:51,300
+depressor و بنعمل كلمة مرة illumination مرة أخر في
+
+444
+00:31:51,300 --> 00:31:57,300
+ضوء بيضوي الفموبنمرر ال .. ال .. السواق دون أن
+
+445
+00:31:57,300 --> 00:32:01,640
+نلمس أي مكان آخر في فم لأن الفم عشرة عشرة أس تسعة
+
+446
+00:32:01,640 --> 00:32:05,060
+عشرة أس اتناش من البكتيريا فنمنع نلمس أي مكان سواء
+
+447
+00:32:05,060 --> 00:32:08,680
+من التونسل و بنضغط على التونسل و بنلف و بنطلع
+
+448
+00:32:08,680 --> 00:32:17,920
+بسرعة دون أن نلمس أخدنا مسحة من التونسل بنجص قطعة
+
+449
+00:32:17,920 --> 00:32:21,120
+القطن و بنحطها في سائل لل extraction تبعي ال
+
+450
+00:32:21,120 --> 00:32:24,950
+antigen بيدوب البكتيريابطلنا مين الآن؟ ال antigen
+
+451
+00:32:24,950 --> 00:32:29,430
+خدوا بالكوا ال antigen الآن هو عبارة عن fragment
+
+452
+00:32:29,430 --> 00:32:35,770
+من البكتيريا و ال antibody أصغر أصلا من البكتيريا
+
+453
+00:32:35,770 --> 00:32:40,870
+فارتباط صغير مع صغير مش هنشوفه،
+
+454
+00:32:40,870 --> 00:32:46,010
+بدنا carrierبروحوا لما بيعطونا ال antibody ك
+
+455
+00:32:46,010 --> 00:32:48,490
+reagent اللي بيقونا إياه في الشركات، بيعطونا مش
+
+456
+00:32:48,490 --> 00:32:52,470
+antibody هيك لحاله، بيعطونا antibody مركّب على ..
+
+457
+00:32:52,470 --> 00:32:56,170
+مركّب على latex beads أو particles، إيش هم ال
+
+458
+00:32:56,170 --> 00:33:01,670
+latex دولة؟ عبارة عن كوريات من ال plastic صغيرة
+
+459
+00:33:01,670 --> 00:33:06,370
+تشبه في حجمها ال RBCs، بتعرف ال RBCs؟ بتعرف حجم ال
+
+460
+00:33:06,370 --> 00:33:14,290
+RBCs؟7.5 micron فاحنا بنركب ال antibody على ال
+
+461
+00:33:14,290 --> 00:33:19,950
+latex هذه عشان هو مالوش اي فايد اخدوا بالكوا
+
+462
+00:33:19,950 --> 00:33:23,530
+الفايد هو انه to visualize the reaction جعل
+
+463
+00:33:23,530 --> 00:33:27,090
+التفاعل اللي سمنا agglutination اللي درستوه في
+
+464
+00:33:27,090 --> 00:33:29,850
+أحياء اللي درستوه في أحياء اللي هو لما عملتوا
+
+465
+00:33:29,850 --> 00:33:35,940
+حصائل الدمفصار لدن هو يبقى عن أنتجين ال antibody
+
+466
+00:33:35,940 --> 00:33:38,720
+إذا توافق ال أنتجين مع ال antibody بيعمل
+
+467
+00:33:38,720 --> 00:33:42,940
+agglutination صح؟ clumping فهذا اسمه agglutination
+
+468
+00:33:42,940 --> 00:33:47,140
+reaction عن مين كشفنا احنا؟ كشفنا عن ال antigen
+
+469
+00:33:47,140 --> 00:33:50,680
+تبع البكتيريا اللي اسمها streptococcus biogenes
+
+470
+00:33:50,680 --> 00:33:54,680
+إذا ال antigen موجود يبقى البكتيريا موجود غير عن
+
+471
+00:33:54,680 --> 00:33:58,640
+ال antibody و إن ال antibody موجود يمكن البكتيريا
+
+472
+00:33:58,640 --> 00:34:05,960
+كانت موجودة زمان صح؟وغان منه لم يصب بالتنسولايتس
+
+473
+00:34:05,960 --> 00:34:10,040
+فلو أنا أخدت منكوا أينات الآن من الدم وفحصت عن ال
+
+474
+00:34:10,040 --> 00:34:15,600
+antibody معظمكوا حالجي boosted لأن
+
+475
+00:34:15,600 --> 00:34:19,280
+ال antibody موجود، بس مش من ال infection حالية، من
+
+476
+00:34:19,280 --> 00:34:22,980
+ال infection السادة، هاي اختلاف بين كلمة antigen
+
+477
+00:34:22,980 --> 00:34:26,520
+وكلمة antibody في هذه الحالة إن وجود ال antigen هو
+
+478
+00:34:26,520 --> 00:34:28,780
+مؤشر لوجود ال microbe نفسه
+
+479
+00:34:31,940 --> 00:34:35,320
+طيب من التقنيات الحديثة في ال rapid diagnosis حاجة
+
+480
+00:34:35,320 --> 00:34:40,760
+اسمها ال bacterial DNA sequences الآن
+
+481
+00:34:40,760 --> 00:34:43,480
+البكتيريا بتختلف عن بعضها و عشانك أعطانا أسماء
+
+482
+00:34:43,480 --> 00:34:47,900
+مختلفة ما الذي يجعل البكتيريا X مختلفة عن Y؟ مش
+
+483
+00:34:47,900 --> 00:34:53,920
+مادتها ورعاتية الهوية الأدق إن في التعرف هي المادة
+
+484
+00:34:53,920 --> 00:35:00,560
+الورعاتية ال Lكل الميكروبات الطبية تقريبا بنسبة 99
+
+485
+00:35:00,560 --> 00:35:05,340
+% كل العلماء أولدوا لها ما يسمى ب unique sequence
+
+486
+00:35:05,340 --> 00:35:10,640
+كلمة unique يعني خريط، مميز، هذا ال sequence مميز
+
+487
+00:35:10,640 --> 00:35:15,260
+البكتيريا هذه عن البكتيريا الأخرى وبالتالي طالع
+
+488
+00:35:15,260 --> 00:35:18,580
+بعيد ال unique sequence لكل موضوع نوع البكتيريا
+
+489
+00:35:18,580 --> 00:35:20,880
+المعروف يبقى ان انا بقدر اشتغل عليها
+
+490
+00:35:20,880 --> 00:35:27,550
+identification كانت بتواجهين مشكلةإنه كيف بدي أكشف
+
+491
+00:35:27,550 --> 00:35:32,730
+عن مادة و .. عن مادة وراتية لميكروب واحد كمية
+
+492
+00:35:32,730 --> 00:35:38,290
+قليلة يصعب الكاشف دخل نظام ال PCR اللي هو
+
+493
+00:35:38,290 --> 00:35:41,870
+Polymerase Chain Reaction وحل هذا المشكلة قاللي
+
+494
+00:35:41,870 --> 00:35:46,070
+إذا أنت عندك one copy one copy من ال DNA اللي بدك
+
+495
+00:35:46,070 --> 00:35:50,030
+إياه أنا بخليلك إياه خلال خمس ساعات، ست ساعات،
+
+496
+00:35:50,030 --> 00:35:52,610
+بخليك إياه مليارات من ال copies كيف؟
+
+497
+00:35:54,770 --> 00:35:58,630
+عنا هاي المادة الوراثية خلينا على اللوحة طول
+
+498
+00:35:58,630 --> 00:36:03,450
+الفترة ان شاء الله هاي المادة الوراثية القطعة
+
+499
+00:36:03,450 --> 00:36:06,870
+الصغيرة هادي هي الـ Nucleotide الـ Nucleotidات
+
+500
+00:36:06,870 --> 00:36:10,410
+تمام؟ و القطعة الحمراء هادي بيسموها Primers
+
+501
+00:36:10,410 --> 00:36:13,950
+Primers هي عبارة عن Sequence Complementary خدوا
+
+502
+00:36:13,950 --> 00:36:18,210
+بالكوا، ده اللي ماأمرش أعرفه ال PCR أنا عارف ال
+
+503
+00:36:18,210 --> 00:36:23,420
+sequence تبع المادة اللي بديها، مظبوط؟ عارففبقى
+
+504
+00:36:23,420 --> 00:36:29,020
+بصمم قطعة صغيرة من complementary DNA لـ 3 prime
+
+505
+00:36:29,020 --> 00:36:32,360
+المنطقة هذه و لـ 3 prime المنطقة هذه هذا بيسميه
+
+506
+00:36:32,360 --> 00:36:36,060
+Primer ليش ال Primer المهم؟ بدونه ال RNA
+
+507
+00:36:36,060 --> 00:36:39,580
+Polymerase .. عفوا ال DNA Polymerase مابيشتغلش
+
+508
+00:36:39,580 --> 00:36:46,580
+الآن أول خطوة عملناdenaturation فسخنا اللي هم
+
+509
+00:36:46,580 --> 00:36:50,320
+المادة اللي وراتيها عن بعضها من بعض بعدين عملنا ما
+
+510
+00:36:50,320 --> 00:36:53,720
+يسمى ب ال primer annealing تمام اللي يسميه
+
+511
+00:36:53,720 --> 00:36:57,880
+annealing و لحظة العملية بتتم عليها على different
+
+512
+00:36:57,880 --> 00:37:02,400
+temperatures بعد ال primer annealing اتى ال DNA
+
+513
+00:37:02,400 --> 00:37:08,460
+polymerase وكمل اللي هو ال elongation process يعني
+
+514
+00:37:08,460 --> 00:37:09,760
+كمل القطعة
+
+515
+00:37:15,950 --> 00:37:20,190
+لو قدنا التلات خطوات مرة أخرى أكم قطعة سياران دي؟
+
+516
+00:37:20,190 --> 00:37:24,730
+أربعة لإنه في عندي واحدة صارت تنتين، تنتين صاروا
+
+517
+00:37:24,730 --> 00:37:30,550
+أيه؟ أربعة فاكرين السيارة اللي هي السيارة اللي
+
+518
+00:37:30,550 --> 00:37:33,530
+كنتوا هتشتروها و تنغشوا في الموضوع و تدفعوا فلوس
+
+519
+00:37:33,530 --> 00:37:36,410
+كتير في هذا الموضوع اتنين، أربعة، تمانين، ستاشرين،
+
+520
+00:37:36,410 --> 00:37:39,850
+تلاتين، أربعة و ستين إلى أرقام ضخمة جدا خلال
+
+521
+00:37:39,850 --> 00:37:44,410
+within hours و طبعا اليوم بجانا زمان نمسك الأنبوبة
+
+522
+00:37:45,350 --> 00:37:49,350
+و نسخنها و نبردها اليوم فيه جهاز صغير هلجد اسمه
+
+523
+00:37:49,350 --> 00:37:53,230
+thermal cycler بيجيهم بالمهمة و بنبرمج و احنا برد
+
+524
+00:37:53,230 --> 00:37:58,370
+سخن عسب المادة اللي بدنايها و بكل سهولة بيعملنا ال
+
+525
+00:37:58,370 --> 00:38:03,610
+cycle طبعا هدا عمل detection هو ماعملش detectionهو
+
+526
+00:38:03,610 --> 00:38:07,390
+على حاجة واحدة amplification القطعة الواحدة أو
+
+527
+00:38:07,390 --> 00:38:11,690
+القطعة الصغيرة خلّاها قطعة كبيرة لكن سهل علينا
+
+528
+00:38:11,690 --> 00:38:15,450
+عملية ال detection لما يكون قطعة كبيرة يكون ال
+
+529
+00:38:15,450 --> 00:38:19,530
+detection سهل طرق ال detection عاد مختلفة ومتنوعة
+
+530
+00:38:23,720 --> 00:38:26,900
+ك .. ك method سريعة فاكرين ان احنا قولنا قبل شوية
+
+531
+00:38:26,900 --> 00:38:31,380
+ال acid fast stain could be used لمين؟ ل .. ل .. ل
+
+532
+00:38:31,380 --> 00:38:34,100
+.. ل ال mycobacterium tuberculosis او حتى ل ال
+
+533
+00:38:34,100 --> 00:38:38,000
+mycobacterium library اللي هي ال U ده أساسا الفحص
+
+534
+00:38:38,000 --> 00:38:41,680
+الأساسي الوحيد لل U ده هو ال acid fast stain فلابد
+
+535
+00:38:41,680 --> 00:38:45,300
+method هو كده والصورة لا .. لا أدري إن كانت كتير
+
+536
+00:38:45,300 --> 00:38:51,090
+واضحة لكن ها لإنها اللي هو البسلية شايفينها؟طبعا
+
+537
+00:38:51,090 --> 00:38:53,890
+هي بتوصف ال .. ال .. ال acid phosphate بصراحة انها
+
+538
+00:38:53,890 --> 00:38:58,090
+thin very thin ان هذا الصورة مكبر اكتر من الصيانات
+
+539
+00:38:58,090 --> 00:39:00,390
+تحت ال microscope بتشوفها اشهر جدا بيشوفوها اصغر
+
+540
+00:39:00,390 --> 00:39:05,810
+شوية احنا
+
+541
+00:39:05,810 --> 00:39:09,650
+أخدنا serology و عملنا detection ل antigen من ال
+
+542
+00:39:09,650 --> 00:39:15,230
+throat swab صح؟ الان ممكن ناخد عين الدم عين الدم و
+
+543
+00:39:15,230 --> 00:39:20,190
+ناخد منها ال serumوفي السيرام بندور على إيش؟ بندور
+
+544
+00:39:20,190 --> 00:39:23,930
+على ال antibody و أعطيكم مثال على مرض بتشيخ قصفيات
+
+545
+00:39:23,930 --> 00:39:30,730
+بطريقة تعرفوا ريكيزيا؟ الريكيزيا انت بعد ما تقولوا
+
+546
+00:39:30,730 --> 00:39:34,270
+ريكيزيا ده بتقولوا على رأيك كلاميديا لإنه تنتن هو
+
+547
+00:39:34,270 --> 00:39:37,450
+plant intracellular برضها الريكيزيا بتسبب بعض
+
+548
+00:39:37,450 --> 00:39:39,770
+الأمراض من ضمن الأمراض الحالي بيسموها الtyphus
+
+549
+00:39:39,770 --> 00:39:45,300
+fever مش typhoid الtyphus هم الtyphusفي غزة طريقة
+
+550
+00:39:45,300 --> 00:39:53,100
+التشخيص الوحيدة لحمة Tefas هو فحص اسمه Ox-19 Ox-19
+
+551
+00:39:53,100 --> 00:39:58,380
+عبارة عن أنتجين بنكشف فيه عن ال antibody في دم
+
+552
+00:39:58,380 --> 00:40:03,000
+اللي هو المرض، هاي التشخيص المتاح الوحيد في قطاع
+
+553
+00:40:03,000 --> 00:40:09,260
+غزة، امام؟ هتقولولي ان ال antibody يمكن تكون من
+
+554
+00:40:09,260 --> 00:40:12,720
+إصابة سابقة إذا الفحص طلع بوسطه، صح؟ يمكن كلها
+
+555
+00:40:12,720 --> 00:40:19,850
+بوسعيةويمكن من إصابة حاليا مين اللي بيقرر انا بعمل
+
+556
+00:40:19,850 --> 00:40:23,230
+test و اعمل deduction و باللي بيقرر مين الطبيب
+
+557
+00:40:23,230 --> 00:40:27,110
+based on the clinical picture بتكونش تنص في يوم من
+
+558
+00:40:27,110 --> 00:40:33,350
+الأيام انه الطبيب حادق و عنده معلومات من خلالها مش
+
+559
+00:40:33,350 --> 00:40:37,230
+بس من .. يعني هو بيستخدم خدماتك diagnostic
+
+560
+00:40:37,230 --> 00:40:42,480
+services للمساعدة في التشخيص و ليس أداة رئيسيةفي
+
+561
+00:40:42,480 --> 00:40:45,900
+التشخيص وبالتالي هو يمتل ال clinical picture صورة
+
+562
+00:40:45,900 --> 00:40:49,240
+سريرية عن المريض قد تساعده لما بيعمل differential
+
+563
+00:40:49,240 --> 00:40:52,460
+diagnosis بيقول المرض هدا احتمال X او Y تعالى
+
+564
+00:40:52,460 --> 00:40:58,120
+نعمله فحص فاحنا بنساعده بنرجحله بعض اللي هو العمل
+
+565
+00:40:58,120 --> 00:41:03,430
+بنأكل معلومات واحدةعشانك انا بقول دايما و ابدا
+
+566
+00:41:03,430 --> 00:41:09,910
+احنا ليسنا في مجال التشخيص احنا في مجال مساعدة في
+
+567
+00:41:09,910 --> 00:41:13,610
+التشخيص وبتالي اياك انك في يوم من الأيام تتجرأ
+
+568
+00:41:13,610 --> 00:41:17,910
+وتعطي نتائج او تناقش نتائج مع المرض طبعا انا
+
+569
+00:41:17,910 --> 00:41:22,430
+بقولكوا الأمر مش سهل احنا مجتمعنا بيدغط على فني
+
+570
+00:41:22,430 --> 00:41:25,250
+المقتبر بيدغط على فني المقتبر او الخصائص التحيات
+
+571
+00:41:25,250 --> 00:41:29,690
+الطبية ان يفسرله النتائج وانا عملت في مجال التحيات
+
+572
+00:41:29,690 --> 00:41:34,040
+الطبية لفترةوكنت اواجه بعض ال .. ال .. ال .. يعني
+
+573
+00:41:34,040 --> 00:41:37,060
+الضغوطات من الناس لكن كنا ماخدين قرار في المكان
+
+574
+00:41:37,060 --> 00:41:40,700
+اللي بنشتغل فيه ممنوع مناقشة نتائج مع المرضى، فكان
+
+575
+00:41:40,700 --> 00:41:44,740
+افضل وسيلة انه ال .. النتائج تغلق في الظرف
+
+576
+00:41:47,560 --> 00:41:51,700
+يفتح بعض .. بعض منهم يفتح الضغط يقرأ يرجع طب بس
+
+577
+00:41:51,700 --> 00:41:55,260
+قوللي إيش فيه مابيش فيه احنا مابنفهم في تفسير
+
+578
+00:41:55,260 --> 00:41:58,920
+النتائج .. تفسير النتائج على مين على الطبيعة طبعا
+
+579
+00:41:58,920 --> 00:42:03,140
+الضغوطات هنا في المجتمع بتولد من إنه يعني أنت دارس
+
+580
+00:42:03,140 --> 00:42:07,460
+أربع سنوات أو خمس سنوات و مابتعرفش أه مابعرفش مافي
+
+581
+00:42:07,460 --> 00:42:11,620
+.. it is easy to say مابعرفش بأدب طبعا واحترام لكن
+
+582
+00:42:11,620 --> 00:42:15,840
+في الآخر بدنا نعرف إنه أي كلام ممكن نقوله احنا
+
+583
+00:42:15,840 --> 00:42:21,230
+ممكن يؤدي إلىيعني اتجاهات متنوعة، اعطيكوا مثل،
+
+584
+00:42:21,230 --> 00:42:25,830
+فحصك نظيف، لو قلنا للمريض فحصك نظيف، ايه مفهوم من
+
+585
+00:42:25,830 --> 00:42:29,130
+نظيف يعني؟ ان هو خالي، هو اصلا هذا الفحص هو اللي
+
+586
+00:42:29,130 --> 00:42:31,530
+بتشخصه يا إما عيال يا إما مش عيال، يمكن مايرجعك
+
+587
+00:42:31,530 --> 00:42:35,350
+للطبيب و يروح على البيت، مايرجعك للطبيب، فحصك
+
+588
+00:42:35,350 --> 00:42:38,730
+negative، فحصك negative، لازم الطبيب يعرف إيش
+
+589
+00:42:38,730 --> 00:42:39,690
+المشكلة اللي عندك
+
+590
+00:42:42,600 --> 00:42:46,420
+وبالتالي حديثك مع المريض قد يؤذي عملية التشخيص
+
+591
+00:42:46,420 --> 00:42:49,600
+وبالتالي نرجو من حضراتكم إنكم من الأن تاخدوا قرار
+
+592
+00:42:49,600 --> 00:42:52,000
+وعادة لنفسكم إنكم ما تناقشوا
+
+593
+00:42:56,090 --> 00:42:59,150
+طيب احنا كنا .. احنا بدأنا بالعلاج اللي قلنا في ال
+
+594
+00:42:59,150 --> 00:43:03,850
+profilaxis لكن لا بأس ان يكون لنا فكرة كيف او ايش
+
+595
+00:43:03,850 --> 00:43:07,810
+هي التقنيات المستخدمة في ال profilaxis profilaxis
+
+596
+00:43:07,810 --> 00:43:12,430
+يعني في انا قائمة من الأمراض بيسموها vaccine
+
+597
+00:43:12,430 --> 00:43:16,690
+preventable diseases الأمراض اللتي يمكن منعها بال
+
+598
+00:43:16,690 --> 00:43:21,480
+vaccinationعن ان ناخد أمثلة تيتانوس، Pertosis،
+
+599
+00:43:21,480 --> 00:43:25,620
+Diphtheria، Measles، Rubella، هد أسمك و لأ الدولة
+
+600
+00:43:25,620 --> 00:43:28,780
+دفتر التطيب تبعك و اللي صغير، اللي و أنتوا صغار،
+
+601
+00:43:28,780 --> 00:43:32,100
+هتلاجوا مجموعة كبيرة من الأمراض البكتيرية و
+
+602
+00:43:32,100 --> 00:43:36,920
+البيروسية مُنِعت تقريبا أو مُنِع درارها اللي كان
+
+603
+00:43:36,920 --> 00:43:41,220
+يقتل بنسب كبيرة مُنِع درارها تقريبا و أصبح إلى ..
+
+604
+00:43:41,220 --> 00:43:43,460
+يعني في البعض .. نشير الأطفال تقريبا في فلسطين
+
+605
+00:43:43,460 --> 00:43:48,240
+Zero من التمانينات، القرن الماضيالـ Tetanus
+
+606
+00:43:48,240 --> 00:43:52,760
+تقريباً Zero يعني فينا أموات كثيرة بالفعل انخفضت
+
+607
+00:43:52,760 --> 00:43:56,920
+مستوياتها إلى الصفر بفعل ال vaccination أنواع ال
+
+608
+00:43:56,920 --> 00:44:00,420
+vaccine inactivated vaccines يعني تحتوي على
+
+609
+00:44:00,420 --> 00:44:06,760
+بكتيريا ميتة أو فيروسات ميتة attenuated يعني مضعفة
+
+610
+00:44:06,760 --> 00:44:11,080
+يعني بكتيريا عرضناها لإجراءات جعلناها غير قادرة
+
+611
+00:44:11,080 --> 00:44:18,060
+على إحداث المرض لكنهذا .. هذا .. هذا أحد عيوبه أنه
+
+612
+00:44:18,060 --> 00:44:21,940
+ممكن البكينة الضعيفة إذا لقت البيئة المناسبة إنها
+
+613
+00:44:21,940 --> 00:44:27,180
+ت .. ترجع لشراسة تبعتها التوكسويد العظيم اللي احنا
+
+614
+00:44:27,180 --> 00:44:31,840
+ذكرناه لكوا في ال Exoxin اللي حاولنا إنه نشيل من
+
+615
+00:44:31,840 --> 00:44:38,320
+صفة ال toxicity و نفلي ال antigen أحلى حاجة في هذا
+
+616
+00:44:38,320 --> 00:44:43,530
+ال vaccine الأخير هذا أو الرابع ال auto vaccineA
+
+617
+00:44:43,530 --> 00:44:45,550
+vaccine prepared from cultures of organisms
+
+618
+00:44:45,550 --> 00:44:48,210
+isolated from patients on tissue or secretions
+
+619
+00:44:48,210 --> 00:44:51,890
+يعني أخدنا البكتيريا اللي أعدلناها من فلان
+
+620
+00:44:51,890 --> 00:44:58,450
+وعلجناها إما قتلناها أو ضعفناها و رجعناها للمرض
+
+621
+00:44:58,450 --> 00:45:02,170
+Auto Vaccine الأخير اللي هو ال polysaccharide
+
+622
+00:45:02,170 --> 00:45:05,710
+vaccine و احنا نتكلم في غالب الأمر عن اللي هو ال
+
+623
+00:45:05,710 --> 00:45:09,690
+capsular polysaccharide في الغالب أعطيكم مثل على
+
+624
+00:45:09,690 --> 00:45:13,910
+capsular polysaccharideمهم جدا وشهير جدا اللى هو
+
+625
+00:45:13,910 --> 00:45:19,350
+ال capsule تبعت ال nyseria meningitis و ال capsule
+
+626
+00:45:19,350 --> 00:45:22,790
+تبعت ال streptococcus pneumonia و ال capsule تبعت
+
+627
+00:45:22,790 --> 00:45:25,950
+ال hemophilus influenza هدول أخدوا بالكوا هتلاقوهم
+
+628
+00:45:25,950 --> 00:45:33,110
+بس أنا بتكررهم مع بعض كتير هحكيهم كمان مرة نيسيريا
+
+629
+00:45:33,110 --> 00:45:37,090
+مينيجدايدس streptococcus pneumonia
+
+630
+00:45:39,960 --> 00:45:46,660
+يمو فلس انفلونزا وخميرة
+
+631
+00:45:46,660 --> 00:45:54,080
+اسمها Cryptococcus ايش معنى هدول الأربع؟ هدول نجوم
+
+632
+00:45:54,080 --> 00:45:58,860
+نجوم في عالم meningitis يعني بسبب meningitis بشكل
+
+633
+00:45:58,860 --> 00:46:07,370
+شائع كلهم بيتميزوا أنهم بوريسا كرايد كابسولاللي هم
+
+634
+00:46:07,370 --> 00:46:11,550
+polysaccharide capsule بنستخدم ال capsule تبعتهم
+
+635
+00:46:11,550 --> 00:46:15,730
+في شغلتين في ال diagnosis نكشف عنها ك antigen و
+
+636
+00:46:15,730 --> 00:46:18,590
+بنستخدمها كمان في ال vaccine preparation بنحضر
+
+637
+00:46:18,590 --> 00:46:22,970
+منها vaccines و بنحكم فيها المرضى عشان نمنع حدوث
+
+638
+00:46:22,970 --> 00:46:27,650
+المرضى طبعا
+
+639
+00:46:27,650 --> 00:46:30,590
+هذا ليه دول مش special حفظ بس هذا بعطيكوا فكرة على
+
+640
+00:46:30,590 --> 00:46:35,710
+أن كل دولة في العالم عندها عندها قائمة قائمة
+
+641
+00:46:37,610 --> 00:46:45,330
+بالأمراض اللى بتطعم فيها او بتحمي أطفالها من خلال
+
+642
+00:46:45,330 --> 00:46:49,910
+ال vaccine منها فمثلا عندنا Hepatitis B Erotavirus
+
+643
+00:46:49,910 --> 00:46:53,150
+فى عندنا Diphtheria Tetanus Pertosis فى عندنا
+
+644
+00:46:53,150 --> 00:46:56,610
+Hemophilus Influenza Type B Hemophilus Influenza
+
+645
+00:46:56,610 --> 00:46:59,270
+لسه الحين حاكينا عنها احنا مظهور Hemophilus
+
+646
+00:46:59,270 --> 00:47:01,290
+Influenza Hemophilus Influenza Hemophilus
+
+647
+00:47:01,290 --> 00:47:01,450
+Influenza Hemophilus Influenza Hemophilus
+
+648
+00:47:01,450 --> 00:47:01,490
+Influenza Hemophilus Influenza Hemophilus
+
+649
+00:47:01,490 --> 00:47:01,670
+Influenza Hemophilus Influenza Hemophilus
+
+650
+00:47:01,670 --> 00:47:03,250
+Influenza Hemophilus Influenza Hemophilus
+
+651
+00:47:03,250 --> 00:47:03,650
+Influenza Hemophilus Influenza Hemophilus
+
+652
+00:47:03,650 --> 00:47:06,010
+Influenza Hemophilus Influenza Hemophفين ال polio
+
+653
+00:47:06,010 --> 00:47:09,950
+virus؟ و ال polio أنا قولتلكوا أنه فلسطين خالية من
+
+654
+00:47:09,950 --> 00:47:15,610
+ال polio من أكتر من 25 سنة تمام؟ ف .. جيد كتير إنه
+
+655
+00:47:15,610 --> 00:47:19,430
+نعرف هذا الكلام missiles, mumps, rubella في
+
+656
+00:47:19,430 --> 00:47:22,190
+الأددية أن أنتوا أخدتوا ماهو ال vaccine؟ في
+
+657
+00:47:22,190 --> 00:47:25,130
+الأددية أخدتوا vaccine؟ عارفين ماهو ال vaccine
+
+658
+00:47:25,130 --> 00:47:30,570
+اللي أخدته؟ لأ ال rubella ال rubella اللي هي
+
+659
+00:47:30,570 --> 00:47:35,130
+الحصبة الألمانيةهذه خطيرة بتعمل complications في
+
+660
+00:47:35,130 --> 00:47:38,230
+ال pregnancy احنا عندنا في البلدان العربية ان سن
+
+661
+00:47:38,230 --> 00:47:40,950
+الزواج بيكون مخافض قد يبدأ من الأددية، مظبوط ولا
+
+662
+00:47:40,950 --> 00:47:44,530
+لا؟ قد يبدأ من الأددية فعشان هيك حطوا السن ..
+
+663
+00:47:44,530 --> 00:47:47,510
+شوفوا احنا بتعملوا في المبادرة الحالية السن تبع ال
+
+664
+00:47:47,510 --> 00:47:51,530
+vaccination طبعا انتوا أخدتواه انتوا صغار، بس
+
+665
+00:47:51,530 --> 00:47:54,750
+عملناله جرعة داعمة وانتوا كبار، تمام؟ وانتوا كبار
+
+666
+00:47:54,750 --> 00:48:00,080
+عملناله جرعة داعمةالفيروس سن ال vaccination له
+
+667
+00:48:00,080 --> 00:48:03,300
+علاقة بعض الأحيان ببعض ال behaviors في البلدان
+
+668
+00:48:03,300 --> 00:48:10,100
+الغربية بيطعموا البنات الجناية من سن ال 11 ل 12 ضد
+
+669
+00:48:10,100 --> 00:48:11,900
+فيروس اسمه
+
+670
+00:48:14,220 --> 00:48:18,220
+Papilloma virus السن هذه بالذات هي بالنسبة لهم
+
+671
+00:48:18,220 --> 00:48:21,760
+السن الناشطة جنسية تمام؟ وبالتالي فيه هذا الفيروس
+
+672
+00:48:21,760 --> 00:48:25,320
+بسبب مرض بينتقل عن طريق الجنس و قد يسبب cancer
+
+673
+00:48:25,320 --> 00:48:30,140
+فانت سبب دي ايه؟ cancer فاختاروا السن هذا للتطييب
+
+674
+00:48:30,140 --> 00:48:35,240
+عشان هو السن اللي ايه الملائم للحماية من المرض
+
+675
+00:48:35,460 --> 00:48:39,400
+انتوا اختاروا سن الاددية عشان سن الزواج بقولنا انه
+
+676
+00:48:39,400 --> 00:48:45,620
+لو صار حامل والست ماعندهاش immunity جيدة لرنبالا
+
+677
+00:48:45,620 --> 00:48:49,880
+قد يحدث complication وقد يحدث اما congenital
+
+678
+00:48:49,880 --> 00:48:54,360
+abnormalities او حتى abortion طبعا تلاحظوا هنا
+
+679
+00:48:54,360 --> 00:48:58,370
+birthone month, two month, four month يبقى فينا
+
+680
+00:48:58,370 --> 00:49:01,650
+هنا يعني اللي هي الفترة الزمنية و .. و كام جرعة
+
+681
+00:49:01,650 --> 00:49:05,810
+بناخد مثلا ال hemophilus influenza type B هكام
+
+682
+00:49:05,810 --> 00:49:11,130
+جرعة؟ عشرين و أربع شهور و إيه؟ ست شهور و ممكن نعطي
+
+683
+00:49:11,130 --> 00:49:14,530
+كمان جرعة دايمة ما بين اتناشر و خمساشر شهر و ما
+
+684
+00:49:14,530 --> 00:49:18,650
+بين تسعتاشر اتناشر يعني في عندي ..اما specific
+
+685
+00:49:18,650 --> 00:49:22,770
+period او range of period في جرعات رئيسية و في
+
+686
+00:49:22,770 --> 00:49:27,110
+حالة بيسميها booster dose بوستر لجرعة الدائمة جرعة
+
+687
+00:49:27,110 --> 00:49:30,690
+الدائمة طبعا في كمان vaccination للكبار من 7 ل 10
+
+688
+00:49:30,690 --> 00:49:34,130
+و من 11 ل 12 و من 12 هاي اللي قلتلكوا قبل ان انا
+
+689
+00:49:34,130 --> 00:49:38,430
+جابه شوية اللي هو الهيمن papillomavirus عطوا range
+
+690
+00:49:38,430 --> 00:49:40,330
+من 11 ل 12 سنة
+
+691
+00:49:42,690 --> 00:49:45,990
+طبعا ال artificial passive immunization تقريبا نفس
+
+692
+00:49:45,990 --> 00:49:49,670
+الأشياء بس بدل ما نعطي vaccine نعطي immunotherapy
+
+693
+00:49:49,670 --> 00:49:53,490
+بالنسبة لل treatment احنا ندور واحد في ال
+
+694
+00:49:53,490 --> 00:49:58,190
+treatmentاللي هو اعطاه صورة للمريض .. للطبيب عفواً
+
+695
+00:49:58,190 --> 00:50:02,630
+عن ال microbe اللي عزلناها و عن مدى حساسيته او
+
+696
+00:50:02,630 --> 00:50:06,930
+مقاومته لقائمة و هذه القائمة من الموضوعات الحيوية
+
+697
+00:50:06,930 --> 00:50:12,050
+بدها علم عشان نختارها يعني مش قائمة أشوائية و هيدا
+
+698
+00:50:12,050 --> 00:50:14,850
+هدرسها ان شاء الله معظمها في ال .. ال .. ال
+
+699
+00:50:14,850 --> 00:50:17,130
+diagnostic اللي بيتكلم عن ال treatment لكل microbe
+
+700
+00:50:17,130 --> 00:50:20,530
+من ال microbeهكذا يعني بيكون هنا ان شاء الله
+
+701
+00:50:20,530 --> 00:50:26,210
+محاضرتنا المحاضرة القادمة هبدأ بال micro organs
+
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/LLEOIggQxQs_raw.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/LLEOIggQxQs_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..302dea0251533ca66e34dfb4299ed719b26f1ab6
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/LLEOIggQxQs_raw.srt
@@ -0,0 +1,2820 @@
+1
+00:00:20,430 --> 00:00:23,870
+بسم الله الرحمن الرحيم فصل جديد بعنوان detection,
+
+2
+00:00:24,110 --> 00:00:26,490
+prophylaxis and treatment of bacterial infection
+
+3
+00:00:26,490 --> 00:00:31,590
+قبل ما ابدأ الموضوع بدأ امهد للعنوان انه ليس من
+
+4
+00:00:31,590 --> 00:00:35,110
+وظائفنا احنا في مجلة العلم الطبية المثبرية ان نقوم
+
+5
+00:00:35,110 --> 00:00:38,570
+بالعلاج او يعني
+
+6
+00:00:42,660 --> 00:00:46,620
+ولا ال prophylaxis بينما بالنسبة لل detection لل
+
+7
+00:00:46,620 --> 00:00:51,220
+bacterial infection بينما أن هذا الدور أساسا هو
+
+8
+00:00:51,220 --> 00:00:54,620
+بيساعد في عملية ال diagnosis و بيساعد في عملية ال
+
+9
+00:00:54,620 --> 00:00:58,730
+prophylaxis و بيساعد في عملية ال treatmentهذا يعني
+
+10
+00:00:58,730 --> 00:01:02,970
+انه بيلقي على عتقنا قرابق كبير جدا من حيث انه دقة
+
+11
+00:01:02,970 --> 00:01:07,270
+ال diagnosis لل bacterial infection اليوم اعتقد ان
+
+12
+00:01:07,270 --> 00:01:10,770
+احنا أصبحنا مؤهلين بعد ما درسنا مجموعة من الفصول
+
+13
+00:01:10,770 --> 00:01:15,470
+انه نتكلم عن how bacterial infection are detected
+
+14
+00:01:15,470 --> 00:01:22,630
+in the laboratory هتكلم عن ال process of diagnosis
+
+15
+00:01:22,630 --> 00:01:26,950
+of bacterial infection in generalو هنبدأ ب step
+
+16
+00:01:26,950 --> 00:01:33,930
+number one تذكير دائم بإن عيناتنا ال clinical اللي
+
+17
+00:01:33,930 --> 00:01:38,550
+هي من الإنسان نسميها clinical و عينات من المصاد
+
+18
+00:01:38,550 --> 00:01:41,830
+الأخرى ممكن نسميها environmental يعني ممكن ناخد
+
+19
+00:01:41,830 --> 00:01:47,050
+عينة هوا عينة ميه من البحر من التربة ممكن عينة
+
+20
+00:01:47,050 --> 00:01:50,130
+غذاء نسميها environmental especially إذا نجسمهم من
+
+21
+00:01:50,130 --> 00:01:55,130
+الآن clinical و environmentalو كلهم خدوا فالكوا
+
+22
+00:01:55,130 --> 00:01:58,910
+لإنكوا إذا تذكروا لما درسنا ال infection chain
+
+23
+00:01:58,910 --> 00:02:04,010
+اللي هي سلسلة أو ال cycle وجدنا إنه ال .. ال .. ال
+
+24
+00:02:04,010 --> 00:02:07,410
+carriers ممكن تكون food ممكن تكون ماء ممكن تكون
+
+25
+00:02:07,410 --> 00:02:11,670
+whatsoever حشرات وبالتالي العينات ممكن تكون متنوعة
+
+26
+00:02:11,670 --> 00:02:15,770
+الآن لو دينا تكلمنا عن ال medical technology
+
+27
+00:02:15,770 --> 00:02:19,310
+profession مهنة التحقيق الطبية و تحدثنا عن نوع
+
+28
+00:02:19,310 --> 00:02:22,850
+العينات اللي أنتوا بتتعاملوا معاها معظم الوقتعينات
+
+29
+00:02:22,850 --> 00:02:28,190
+دم عينات urine عينات بعض الحالات body fluid بس في
+
+30
+00:02:28,190 --> 00:02:33,110
+ال microbiology everything can constitute a
+
+31
+00:02:33,110 --> 00:02:36,610
+clinical spacing حتى الشعر حتى الأضفر ممكن يكون
+
+32
+00:02:36,610 --> 00:02:40,310
+عينة لإنه في الآخر could be infected ال skin
+
+33
+00:02:40,310 --> 00:02:45,290
+biopsies organ biopsies all of them can be a
+
+34
+00:02:45,290 --> 00:02:48,710
+spacing عشان هي جسمنا ensemble سباق ال clinical
+
+35
+00:02:48,710 --> 00:02:49,570
+إلى قسمين
+
+36
+00:02:53,190 --> 00:02:56,710
+والصحيحة انا مش مخطط في المحاضرة هذه ان اتكلم عن
+
+37
+00:02:56,710 --> 00:03:00,230
+ال sampling ولا ال samples لان ال samples بحد
+
+38
+00:03:00,230 --> 00:03:05,290
+ذاتها تستغرق مننا محاضرتين او تلاتة عشان نتحدث عن
+
+39
+00:03:05,290 --> 00:03:10,170
+how to collect a good specimen كيف نجمع عينا
+
+40
+00:03:10,170 --> 00:03:13,270
+traceable in a way to avoid contamination with
+
+41
+00:03:13,270 --> 00:03:16,450
+indigenous normal flora بإن الميكروبت باعنا هو
+
+42
+00:03:16,450 --> 00:03:22,620
+اللي نحصل عليهناخد فكرة عن أمثلة عن samples أن
+
+43
+00:03:22,620 --> 00:03:27,600
+body fluids زي blood و urine و cerebrospinal fluid
+
+44
+00:03:27,600 --> 00:03:33,080
+العادة بناخدها بطريقة جيدة و بعدين بنزراحها
+
+45
+00:03:33,080 --> 00:03:36,480
+بنعملها streaming على culture media على سلط
+
+46
+00:03:36,480 --> 00:03:38,940
+culture media في الغالب بعض الأحيان نلجأ ل liquid
+
+47
+00:03:38,940 --> 00:03:44,890
+media لكن mostly على السلط mediaafter incubating
+
+48
+00:03:44,890 --> 00:03:48,530
+the specimen for a certain period of time اللي
+
+49
+00:03:48,530 --> 00:03:52,970
+ممكن تتروح من يوم إلى عدة أيام إلى شهر أو أكتر من
+
+50
+00:03:52,970 --> 00:04:00,930
+ذلك بيظهر عندنا ما يسمى ب ال colony و ال colony هي
+
+51
+00:04:00,930 --> 00:04:06,030
+huge number millions billions of microorganisms
+
+52
+00:04:06,030 --> 00:04:13,790
+جمعت مع بعض نمت تمام فأصبحت ظاهرة ل ال naked eyeمن
+
+53
+00:04:13,790 --> 00:04:18,350
+أجمل الأشياء اللي احنا نمدح فيها ال .. ال .. ال
+
+54
+00:04:18,350 --> 00:04:22,490
+solid media بنقول انه بيوفرلنا إمكانية رؤية
+
+55
+00:04:22,490 --> 00:04:25,290
+المستعمرة ال solid media بينما ال liquid media
+
+56
+00:04:25,290 --> 00:04:28,670
+بتوفرلناهش لإنه النمو فيها بيكون بشكل ايه؟
+
+57
+00:04:28,670 --> 00:04:33,410
+turbidity بيكون بشكل turbidity طيب مال .. مال يعني
+
+58
+00:04:33,410 --> 00:04:38,030
+الممتع ولا مال مفيد في رؤية المستعمرة على ال solid
+
+59
+00:04:38,030 --> 00:04:43,580
+كله على solid plate؟ال solid plate بيوفر لنا
+
+60
+00:04:43,580 --> 00:04:49,620
+مستعمرة، المستعمرة حجمها، لونها، ارتفاعها، شكلها،
+
+61
+00:04:49,620 --> 00:04:54,020
+كلها مرتبطة بنوع البكتيريا، إذا كلمة مرتبطة بنوع
+
+62
+00:04:54,020 --> 00:04:59,000
+البكتيريا إذا هي صفات، والصفات هي اللي احنا
+
+63
+00:04:59,000 --> 00:05:01,880
+استغلناها في عمل التعرف على ال micro organism،
+
+64
+00:05:01,880 --> 00:05:05,120
+مظبوط ولا لأ؟وبالتالي بدنا نعرف ان ال colony
+
+65
+00:05:05,120 --> 00:05:09,860
+morphology، ال size، ال elevation، ال texture أو
+
+66
+00:05:09,860 --> 00:05:14,000
+ال consistency كلها صفات نستخدمها للتعرف على ال
+
+67
+00:05:14,000 --> 00:05:18,360
+microorganism بتعطيك مثال حسابي I would like to
+
+68
+00:05:18,360 --> 00:05:21,440
+describe Staphylococcus aureus colonies on blood
+
+69
+00:05:21,440 --> 00:05:25,720
+agar They are rounded, large, two to four
+
+70
+00:05:25,720 --> 00:05:29,460
+millimeters with golden yellow pigmented colonies.
+
+71
+00:05:30,440 --> 00:05:35,490
+Edges are entireوصفت الان انا اخد بالكوا مستعمرة
+
+72
+00:05:35,490 --> 00:05:42,230
+بلون وحجم وشكل الاستدارة تبع المستعمرة بس ما وصفت
+
+73
+00:05:42,230 --> 00:05:45,810
+انا الوسط او ممكن قولت في سياسة الحديث on blood
+
+74
+00:05:45,810 --> 00:05:51,310
+again يبقى وصف المستعمرة مرتبط بمين؟ بال media
+
+75
+00:05:51,310 --> 00:05:57,350
+اللي نمي عليها ماينفعش اقول description لمستعمرة X
+
+76
+00:05:57,350 --> 00:06:04,400
+الا على وسط Zلش؟ لأن نفس البكتيريا لو زرعناها على
+
+77
+00:06:04,400 --> 00:06:08,960
+وسط تاني قد تختلف شكل مستعمراتها، إذا الوصف مرتبط
+
+78
+00:06:08,960 --> 00:06:15,060
+بمين؟ بنوع الطبق، نوع البكتيريا و نوع الطبق الوصف
+
+79
+00:06:15,060 --> 00:06:19,740
+بتاع المستعمرة مرتبط بالتنين، بالوسط و مرتبط
+
+80
+00:06:19,740 --> 00:06:20,820
+بالكائن نفسه
+
+81
+00:06:23,420 --> 00:06:26,200
+طيب فمن ضمن الأشياء اللي يمكن نستفيد منها خلال
+
+82
+00:06:26,200 --> 00:06:30,120
+فترة الزراعة أنه هل البكتيريا احتلت Oxygen ولا ما
+
+83
+00:06:30,120 --> 00:06:33,600
+احتلت Oxygen؟ يعني هل هي aerobic ولا anaerobic ولا
+
+84
+00:06:33,600 --> 00:06:36,660
+facultative anaerobic ولا micro-aerophilic؟ طبعا
+
+85
+00:06:36,660 --> 00:06:40,120
+مش صفة من صفات، هل احتلت ال room temperature في ال
+
+86
+00:06:40,120 --> 00:06:42,880
+growth ولا ما احتلت ال room temperature؟ انها 37
+
+87
+00:06:42,880 --> 00:06:49,890
+درجة مئوية ولا 42 درجة مئوية؟إذا فيانا صفات أخرى
+
+88
+00:06:49,890 --> 00:06:53,070
+ممكن خلال عملية الزراعة ناخدها زي مين ال oxygen
+
+89
+00:06:53,070 --> 00:06:58,550
+requirement ال temperature ال CO2 requirement هل
+
+90
+00:06:58,550 --> 00:07:02,050
+وضعنا معاها مع ال incubation CO2 و غاز ال CO2 و لا
+
+91
+00:07:02,050 --> 00:07:06,990
+ما وضعناها هذي أيضا أشياء ال pH من ضمن مين من ضمن
+
+92
+00:07:06,990 --> 00:07:10,030
+الطبق نفسه من ضمن الطبق هذا اللي بتكلم عن خارج
+
+93
+00:07:10,030 --> 00:07:13,970
+الطبق احنا الأولانية بنسميها cultural
+
+94
+00:07:13,970 --> 00:07:19,660
+characteristics صفات مزرعيةوبنقسمها لقسمين صفات
+
+95
+00:07:19,660 --> 00:07:25,600
+متعلقة بالمستعبر نفسها تمام وصفات متعلقة بال
+
+96
+00:07:25,600 --> 00:07:30,900
+environment من temperature وحرارة
+
+97
+00:07:30,900 --> 00:07:35,700
+و CO2 ناخد
+
+98
+00:07:35,700 --> 00:07:39,480
+الصورة اللى قدامنا هنا تلخيص وتعبير عما ذكرناه في
+
+99
+00:07:39,480 --> 00:07:43,540
+اللحظة السابقة بس بتفاصيل اكتر عندى ال cup هذه
+
+100
+00:07:43,540 --> 00:07:44,040
+معبرة
+
+101
+00:07:48,990 --> 00:07:52,290
+تم زراعته على طبق زي ما احنا شايفين هنا و بدأت
+
+102
+00:07:52,290 --> 00:07:56,050
+ايه؟ ايه اكتهار عندي مستعمرات و من المستعمرات اللي
+
+103
+00:07:56,050 --> 00:07:59,970
+بنركز عليها لازم تكون well isolated colonies كلمة
+
+104
+00:07:59,970 --> 00:08:03,030
+well isolated colonies يعني منفصلة بشكل جيد بحيث
+
+105
+00:08:03,030 --> 00:08:07,890
+ان اقدر اوصفها لحالها دون ان عن الآخرين و بقدر اخد
+
+106
+00:08:07,890 --> 00:08:12,430
+منها sub culture ايضا لأعمل فحوصات اخرى الآن
+
+107
+00:08:12,430 --> 00:08:16,370
+اللوحة الكبيرة هذه الخضراء اللي ملونة بالأخضر طلع
+
+108
+00:08:16,370 --> 00:08:21,100
+على مستعمرة واحدةمستعمرة واحد لو اتطلعنا عليها من
+
+109
+00:08:21,100 --> 00:08:25,760
+فوق ممكن نجيها منقطة زي ما احنا شايفين هنا ممكن
+
+110
+00:08:25,760 --> 00:08:29,480
+تكون دائرية ممكن تكون filamentous خيطية ممكن تكون
+
+111
+00:08:29,480 --> 00:08:33,700
+irregular ممكن تكون rhizoid عاملة زي اليذور امام
+
+112
+00:08:33,700 --> 00:08:38,720
+او spindle مغزالية لو اتطلعنا عليها ب flat بهذا
+
+113
+00:08:38,720 --> 00:08:43,080
+الشكل يعني هي طبقواطلعنا بهذا المستعمرات على مستوى
+
+114
+00:08:43,080 --> 00:08:47,060
+العين هنشوف مجموعة من ال .. ال .. ال .. الاشياء
+
+115
+00:08:47,060 --> 00:08:51,920
+أخرى زي مثلا هنا flat طبقة رقيقة جدا جدا على ال ..
+
+116
+00:08:51,920 --> 00:08:56,840
+مسطحها على ال .. ال .. الطبق بينما هنا مرتفعة شوية
+
+117
+00:08:56,840 --> 00:09:02,740
+هنا convex هنا ناخدة نصف دائرة تقريبا هنا مقببة
+
+118
+00:09:02,740 --> 00:09:06,650
+أمبونية عاملة زي القبة تمام؟لو جينا طلعنا على
+
+119
+00:09:06,650 --> 00:09:09,710
+الحواف من فوق مرة أخرى طلعنا على المستعمرة بهذا
+
+120
+00:09:09,710 --> 00:09:13,650
+الشكل الحواف تبعتها لما أنا وصفت لستفينيو كوكاس
+
+121
+00:09:13,650 --> 00:09:16,670
+اوريس قلت انها entire edges يبقى بتكلم عن الحواف
+
+122
+00:09:16,670 --> 00:09:21,830
+entire كاملة الاستدارة مش فيها اي تعرج تعرجات
+
+123
+00:09:21,830 --> 00:09:27,430
+undulate مموجة زي ما تشايفين هنا على اللوحة looped
+
+124
+00:09:27,430 --> 00:09:33,830
+مفصصة يعني مقسمة إلى فصوص الجنبها للerosجريمة من
+
+125
+00:09:33,830 --> 00:09:38,950
+مين؟ من لوبيت لكن حجمهم أصغر ما بيسموها مسننة ..
+
+126
+00:09:38,950 --> 00:09:44,830
+مسنن اللي بعدها filamentous و اللي بعدها curled
+
+127
+00:09:44,830 --> 00:09:48,110
+curled معناها مجعنة .. curled من curly .. curly
+
+128
+00:09:48,110 --> 00:09:52,550
+معناها مجعن اللوحة اللي .. او الصورة الأخيرة اللي
+
+129
+00:09:52,550 --> 00:09:56,530
+موجودة في ال .. هذه بتبين أو بتعطينا indication
+
+130
+00:09:56,530 --> 00:09:59,970
+على انه هل البكتينة تهتلت الوسيجين او الـCO2 الى
+
+131
+00:09:59,970 --> 00:10:04,580
+اخر .. دلوقت اخراللوحة اللى أمامنا هذه بتوضح لنا
+
+132
+00:10:04,580 --> 00:10:11,340
+انه سقينة زريعة مستعمرات بدأنا نوصل فيها عرفنا
+
+133
+00:10:11,340 --> 00:10:14,840
+حاجة البكتيريا للاكسجين او ال CO2 او ال
+
+134
+00:10:14,840 --> 00:10:19,680
+temperature من خلالها خطوة تانية و هي الخطوة
+
+135
+00:10:19,680 --> 00:10:23,940
+الرائعة و الأعظم و اللى هنركز عليها نعم هنركز
+
+136
+00:10:23,940 --> 00:10:29,610
+عليها بشكل كبيرالـ Gram stain is the most
+
+137
+00:10:29,610 --> 00:10:33,670
+important technique in conventional microbiology
+
+138
+00:10:33,670 --> 00:10:36,950
+كلمة conventional يعني ال microbiology التقليدية
+
+139
+00:10:36,950 --> 00:10:42,550
+اللي احنا بشتغلها في مختبرات لماذا؟ لأنه بيقسم
+
+140
+00:10:42,550 --> 00:10:46,690
+البكتيريا إلى اتنين ولا اربع انا اليوم احنا اكبرنا
+
+141
+00:10:46,690 --> 00:10:53,210
+شوية من نقسمه لاربع وقسمت عدد كبير من الميكروبات
+
+142
+00:10:53,210 --> 00:10:56,170
+إلى اربع أجزاء أفضل من قسمته إلى جزءين صح؟
+
+143
+00:11:04,720 --> 00:11:08,700
+النص عشرة تلاف العمليات بتتصعب يعني بمعنى اخر لو
+
+144
+00:11:08,700 --> 00:11:14,920
+لاجينا تقنية تقسم البكتيريا الى ستاشر الى اتنين و
+
+145
+00:11:14,920 --> 00:11:18,860
+تلاتين بيكون افضل من ان .. لكن ال gram stem بضربة
+
+146
+00:11:18,860 --> 00:11:23,720
+واحدة بيقسم البكتيريا الى اربع اقصر كيف؟ gram
+
+147
+00:11:23,720 --> 00:11:27,580
+positive و gram negative هي قسمين تحت القسمين
+
+148
+00:11:27,580 --> 00:11:32,260
+الأولينground positive bacilli و ground negative
+
+149
+00:11:32,260 --> 00:11:35,380
+bacilli ground positive cocci و ground negative
+
+150
+00:11:35,380 --> 00:11:38,440
+cocci هاي أربع قسم صراحة اللي بيقولها ال .. ال ..
+
+151
+00:11:38,440 --> 00:11:43,580
+ال .. ال .. ال .. ال spiral organism انسوها الأن
+
+152
+00:11:43,580 --> 00:11:47,820
+خدوا يتكلموا عن مين بس على ال cocci و اللي هم ال
+
+153
+00:11:47,820 --> 00:11:51,300
+bacilli خدوا بالكم القسم اللي أنا ذكرته من الأربع
+
+154
+00:11:51,300 --> 00:11:56,720
+هم العناوين لأي إنسان بيعمل في مجال ال
+
+155
+00:11:56,720 --> 00:12:02,060
+microbiologyأول عنوان بدنا نعرف عنه البكتيريا لازم
+
+156
+00:12:02,060 --> 00:12:06,900
+انها gram stain و morphology او الكلاش عزلنا
+
+157
+00:12:06,900 --> 00:12:09,780
+بكتيريا من X مريض .. من مريض و عملنا لها gram
+
+158
+00:12:09,780 --> 00:12:14,760
+stain طلعت gram negative coccyte الان اللي ..
+
+159
+00:12:14,760 --> 00:12:19,940
+العامل اللي بيحدد اختيار ده الفحوصات هو العنوان
+
+160
+00:12:19,940 --> 00:12:24,020
+الكبيرإذا كانت جرام نيجاتيف كوكساي أول فحص بعمل
+
+161
+00:12:24,020 --> 00:12:28,380
+Oxidase وبعدين بعمل فحصات زي glucose oxidation
+
+162
+00:12:28,380 --> 00:12:34,470
+testإذا كانت gram بصتف بسلعي بعمل كتاليز بعملش
+
+163
+00:12:34,470 --> 00:12:38,890
+وكتاليز إذا مين حدد الفحصات التالية الانواع
+
+164
+00:12:38,890 --> 00:12:40,790
+الرئيسي؟ مين الانواع الرئيسي منجبناه؟ من ال
+
+165
+00:12:40,790 --> 00:12:46,210
+Granstein امام؟ إذا بدون أدنى شك ما زال ال
+
+166
+00:12:46,210 --> 00:12:50,230
+Granstein التقنية الأساسية اللي بنستخدمها في
+
+167
+00:12:50,230 --> 00:12:53,230
+التعرف على ال micro organs وبالتالي أنا بقول ..
+
+168
+00:12:53,230 --> 00:12:56,190
+أنا قولت للطلاب بقولكم المرة .. إيه ال Quran؟إذا
+
+169
+00:12:56,190 --> 00:12:58,930
+انتوا الأحد دلوقت شاعرين ان انتوا مش جادرين
+
+170
+00:12:58,930 --> 00:13:02,290
+تتميزوا بين الـ ground positive و ground negative
+
+171
+00:13:02,290 --> 00:13:06,330
+لأ اطلبوا من المدرسات المعمل انهم يعملوا لكوا
+
+172
+00:13:06,330 --> 00:13:09,530
+review حتى تتأكدوا انكم بالفعل بتعرفوا ال ground
+
+173
+00:13:09,530 --> 00:13:11,130
+positive من ال ground negative بتعرفوا ال بس الله
+
+174
+00:13:11,130 --> 00:13:15,810
+من الكوكساي بتعرفوا ال arrangements تمام؟ مهم جدا
+
+175
+00:13:15,810 --> 00:13:19,850
+المعلومة، فضلوا الحين لما نستخدم ال ground .. ال
+
+176
+00:13:19,850 --> 00:13:22,670
+ground negative تقسم عن ال ground positive و
+
+177
+00:13:22,670 --> 00:13:23,230
+ground negative؟
+
+178
+00:13:26,010 --> 00:13:29,890
+الموضوع استثناء استثناء هذا استثناء احنا ماجداش
+
+179
+00:13:29,890 --> 00:13:32,690
+نشوف اللي ماجداش نصفه لما انا بستخدم ال ground
+
+180
+00:13:32,690 --> 00:13:35,430
+stain يا مابتنصف ال ground بصي يا ماجداش نجد فيه
+
+181
+00:13:35,430 --> 00:13:39,390
+يا مابشوفهاش، مظبوط؟ فبعرفهاش، مادام مشوفتهاش انا
+
+182
+00:13:39,390 --> 00:13:42,830
+في جامعة بعرفهاش فبتطبق عليها تقنية تانية، حيمول
+
+183
+00:13:42,830 --> 00:13:46,050
+معانا كمان شوية بعض التقنيات الموظفة أو اللي
+
+184
+00:13:46,050 --> 00:13:49,270
+بنوظفها لما نلاجي مسائل من النوع هذا، بكتيريا
+
+185
+00:13:49,270 --> 00:13:53,410
+ماجداش ننميها، بكتيريا بطيقة نمو، هنشوف كيف نلاجي
+
+186
+00:13:53,410 --> 00:13:58,630
+حل معاهاطيب قبل ما اطلع من ال grammar stain انا
+
+187
+00:13:58,630 --> 00:14:02,090
+بدي احكي معلومة مهمة جدا ان ال grammar stain ممكن
+
+188
+00:14:02,090 --> 00:14:09,280
+وضفه مرتين فاكرين العينة؟هذه العينة، اعتبروا هذه
+
+189
+00:14:09,280 --> 00:14:13,700
+العينة مش urine، اعتبروها CSF، ليش CSF؟ لأن هو بدأ
+
+190
+00:14:13,700 --> 00:14:17,840
+.. أعطيكوا مؤشر خطورة، أكيد عينة ال urine أقل
+
+191
+00:14:17,840 --> 00:14:22,040
+الخطورة من عينة مين؟ ال CSF ال CSF ال infection في
+
+192
+00:14:22,040 --> 00:14:26,740
+.. في منطقة حساسة جدا و أي خلية عصبية بتموت لم يكن
+
+193
+00:14:26,740 --> 00:14:33,420
+هناك regeneration إلافتعتبر يعني عينة احنا بنسميها
+
+194
+00:14:33,420 --> 00:14:38,760
+emergency specimen، بنوقف كل الشغل و بنشتغل CSF طب
+
+195
+00:14:38,760 --> 00:14:41,720
+أنا جالبا بتكلم عن مزرعة، مزرعة بيتها 24 ساعة و
+
+196
+00:14:41,720 --> 00:14:44,220
+بيتها كمان في فترة 24 ساعة و sensitivity كمان 24
+
+197
+00:14:44,220 --> 00:14:48,440
+ساعة يعني 72 ساعة، هل من المنطق انه نترك المريض
+
+198
+00:14:48,440 --> 00:14:52,300
+بدون علاج لفترة 78 ساعة و أنا بتكلم عن عينة CSF
+
+199
+00:14:54,360 --> 00:14:57,520
+يبقى انا ممكن استخدم او وضف ال gram stain مباشرة
+
+200
+00:14:57,520 --> 00:15:01,980
+على العينة انا اعمل centrifigation للعينة و اخد ال
+
+201
+00:15:01,980 --> 00:15:05,960
+sediment و اعمل ال gram stain من العينة مباشرة من
+
+202
+00:15:05,960 --> 00:15:10,820
+العينة مباشرةإذا ولدت فيها بكتيريا، معناه حق أنه
+
+203
+00:15:10,820 --> 00:15:13,540
+في بكتيريا ال infection، يبقى الطبيب بيعرف أنه في
+
+204
+00:15:13,540 --> 00:15:16,640
+بكتيريا ال infection، جديد الفترة الزمنية اللي
+
+205
+00:15:16,640 --> 00:15:21,680
+أخدتني و أخدتها حتى أصدر نتيجة تقريبا نصف ساعة،
+
+206
+00:15:21,680 --> 00:15:25,480
+فخلال نصف ساعة أنا باعطي للطبيب معلومات أنه في
+
+207
+00:15:25,480 --> 00:15:28,000
+بكتيريا أو مافيش بكتيريا، positive أو negative،
+
+208
+00:15:28,000 --> 00:15:30,520
+وإذا كان في بكتيريا، هل هي gram positive ولا gram
+
+209
+00:15:30,520 --> 00:15:33,680
+negative، وإذا كان positive أو negative، هل هي
+
+210
+00:15:33,680 --> 00:15:37,700
+بصلة ولا oxide، هذا بيساعد الطبيب على أنه يختار
+
+211
+00:15:38,200 --> 00:15:41,500
+more selective therapy لحين ظهور نتيجة علمية
+
+212
+00:15:41,500 --> 00:15:44,940
+للمزارع، إلى ال gramsteria استخدمت وانا مرتين، مرة
+
+213
+00:15:44,940 --> 00:15:49,640
+مباشرة مع ال specimen ومرة في التعرف على البكتيريا
+
+214
+00:15:49,640 --> 00:15:51,460
+بعد زراعتها من المزارع
+
+215
+00:15:54,300 --> 00:15:57,660
+هذه الصورة اللي قدامنا بتوضح أن بعض الأشكال و
+
+216
+00:15:57,660 --> 00:16:01,360
+الألوان لونان مشهورين اللي هو ال pink و ال violet
+
+217
+00:16:01,360 --> 00:16:03,940
+ال violet لل gram positive و ال pink لل gram
+
+218
+00:16:03,940 --> 00:16:24,280
+negative
+
+219
+00:16:26,900 --> 00:16:32,140
+اللون اللي بتاعة هدا؟ ليش هدا؟ blue مثلا يعني؟ اه
+
+220
+00:16:32,140 --> 00:16:34,220
+لو وامكن عندي انا ال pink عنكم انتوا هتصبولا مش
+
+221
+00:16:34,220 --> 00:16:40,110
+مبينبس عموما ال .. ال .. ال .. ال .. فوق هنا
+
+222
+00:16:40,110 --> 00:16:43,450
+ground positive و فيه عندي بسلاي و فيه دبلو كوكاي
+
+223
+00:16:43,450 --> 00:16:48,970
+و فيه عندي chains تمام سلاسل او ستربتو فيه دبلو و
+
+224
+00:16:48,970 --> 00:16:52,890
+فيه عندي هنا clusters بينما هنا فيه دبلو كوكساي
+
+225
+00:16:52,890 --> 00:16:57,110
+ground negative هنا single بسلاي او بالاخر ممكن
+
+226
+00:16:57,110 --> 00:17:01,170
+يسموها cocoa بسلاي لأنها متفقنة و عنها قصيرة هنا
+
+227
+00:17:01,170 --> 00:17:04,690
+فيه عندنا بسلاي اشي بكبسول و اشي بدون كبسول و فيه
+
+228
+00:17:04,690 --> 00:17:10,360
+عندنا هنا longلكن البسلاي أشكال و ألوان مختلفة و
+
+229
+00:17:10,360 --> 00:17:15,440
+اللي حابب أنه يعني يشوف تنوع الميكروبات يجيب عينة
+
+230
+00:17:15,440 --> 00:17:19,380
+sewage مجاري و يحطها و يعمل منها ground stain او
+
+231
+00:17:19,380 --> 00:17:23,200
+يطلع عليها حتى و يتموت و يشوف الأشكال هيشوف spiral
+
+232
+00:17:23,200 --> 00:17:28,260
+و هيشوف coaxial و هيشوف ممكن مربعات كمان يعني rare
+
+233
+00:17:28,260 --> 00:17:33,250
+bacteriaالصورة اللى قدامنا هنا بتوضح انها يمكن
+
+234
+00:17:33,250 --> 00:17:38,030
+بصورة كمان حقيقية هذه تحت ال microscope وبالتالي
+
+235
+00:17:38,030 --> 00:17:41,270
+الآن بدأت المشكلة بدأت المشكلة الصورة هنا ال gram
+
+236
+00:17:41,270 --> 00:17:46,090
+-bacilli gram-booster cocci هنا gram-diplicocci
+
+237
+00:17:46,090 --> 00:17:49,790
+هنا filamentous خيطية اللي أخر كده متشابكة مع
+
+238
+00:17:49,790 --> 00:17:57,250
+بعضها البكتيريا هذا سموها لكن في الآخر اللون تبعها
+
+239
+00:17:57,250 --> 00:18:02,560
+violet ومخدة اللي هو ال gram-booster reactionطيب
+
+240
+00:18:02,560 --> 00:18:06,620
+زرعنا العينة طلع من مستعمرات عملنا gram stain و
+
+241
+00:18:06,620 --> 00:18:08,920
+عرفنا ايش هو ال gram stain تبع البكتيريا what is
+
+242
+00:18:08,920 --> 00:18:14,560
+the next step؟ biochemical identification و هذه
+
+243
+00:18:14,560 --> 00:18:16,960
+الخطوة اللي هتبدأوا فيها في المعمل اعتقد الأسبوع
+
+244
+00:18:16,960 --> 00:18:20,720
+هذا اللي هو ال biochemical tests ال biochemical
+
+245
+00:18:20,720 --> 00:18:24,280
+tests انا سألت المثات اللي في المعمل و قالوا ان
+
+246
+00:18:24,280 --> 00:18:28,740
+الأسبوع هذا هنبدأ في ال biochemical reactionsمن
+
+247
+00:18:28,740 --> 00:18:31,060
+ضمن ال biochemical reaction مجموعة ضخمة من
+
+248
+00:18:31,060 --> 00:18:35,300
+الفحوصات اللي هم زي ال indole و ال methyl red و
+
+249
+00:18:35,300 --> 00:18:39,180
+الbocostroskara و ال citrate مروا معاها فحوصات على
+
+250
+00:18:39,180 --> 00:18:42,740
+فكرة قبل هيك احنا في النظرية لما اتكلمنا عن
+
+251
+00:18:42,740 --> 00:18:44,460
+البكتيري الميتابوليزم ده بتذكر
+
+252
+00:18:48,060 --> 00:18:51,020
+لما بنقول positive و بنقول negative احنا اتفقنا في
+
+253
+00:18:51,020 --> 00:18:55,340
+ال microbiology لا يوجد دا هنا قيمة صفرية يعني حد
+
+254
+00:18:55,340 --> 00:18:58,200
+ما .. مالاش معنى كل إشي له معنى سواء negative أو
+
+255
+00:18:58,200 --> 00:19:01,340
+ال positive إيه له معنى؟ يعني مثلا بنقول الشريشة
+
+256
+00:19:01,340 --> 00:19:05,000
+كلها ان دول positive بنقول ال enterobacter ان دول
+
+257
+00:19:05,000 --> 00:19:08,180
+negative أنا بالنسبة ليه ال negative و ال positive
+
+258
+00:19:08,180 --> 00:19:13,280
+هم صفة غير موجودة و موجودة زي ما ورّح اقولكوا
+
+259
+00:19:13,280 --> 00:19:17,280
+بيلبس نظارات او ما .. مابيلبس نظارات يا صفة موجودة
+
+260
+00:19:17,990 --> 00:19:24,150
+فبتالي وزنها واحد اتنين ووزنهم بالنسبالنا واحد طيب
+
+261
+00:19:24,150 --> 00:19:28,690
+انا بده اعقد الأمور عشان اسهلها في نفس الوقت عندي
+
+262
+00:19:28,690 --> 00:19:35,510
+200 بكتيريا 200 بكتيريا احفظوا 200 اسم 200 اسم 200
+
+263
+00:19:35,510 --> 00:19:39,070
+بكتيريا يعني 200 اسم بكتيريا مختلفة عشان اميزهم مع
+
+264
+00:19:39,070 --> 00:19:45,450
+بعض بده اعملهم كل واحدة عشرين فحصيعني 200 ضرب 20
+
+265
+00:19:45,450 --> 00:19:48,550
+اذا كان positive و negative يطلع معاه رقم ضخم
+
+266
+00:19:48,550 --> 00:19:53,490
+هتصحي و تنامي و انت تقولي positive ولا negative؟
+
+267
+00:19:53,490 --> 00:19:56,210
+negative ولا positive؟ و اليوم .. و اليوم ممكن
+
+268
+00:19:56,210 --> 00:19:59,170
+تحفظيهم يعني في ناس عندهم photo memory و تانية و
+
+269
+00:19:59,170 --> 00:20:03,530
+تكوني نسيهم لذلك ليس من المنطقي انه نحفظ كل
+
+270
+00:20:03,530 --> 00:20:09,390
+الفحوصات لل 220 اللي بناهم كان هذا major
+
+271
+00:20:09,390 --> 00:20:15,240
+differentiation testهذا المين للطلبة و العاملين في
+
+272
+00:20:15,240 --> 00:20:21,560
+المجال لما بنكبر شوية بنبدأ نتعلم بال softwares،
+
+273
+00:20:21,560 --> 00:20:26,600
+بندخل البيانات، بندخل البيانات اللي هم ال 200
+
+274
+00:20:26,600 --> 00:20:31,900
+بكتيريا و ال 20 فحص و نتائجهمتمام؟ و لما بنعزل
+
+275
+00:20:31,900 --> 00:20:35,300
+ميكروبي جديد احنا نتعرف .. نعمله فحصات ايش بيطلع
+
+276
+00:20:35,300 --> 00:20:37,780
+الاتاجوب ندخلهم على الكمبيوتر بيقارنهم بقاعة
+
+277
+00:20:37,780 --> 00:20:41,340
+البيانات بيطلعنا بيقولنا you're bacteria is هذا
+
+278
+00:20:41,340 --> 00:20:45,580
+لأنه احنا فيكم نحفظ بعض الفحصات اللي بيسميناها
+
+279
+00:20:45,580 --> 00:20:51,320
+major differentiation test يعني لما اقول Shigella
+
+280
+00:20:51,320 --> 00:20:55,120
+non motile و Salmonella motile هذا يعني ال
+
+281
+00:20:55,120 --> 00:20:57,280
+differentiation test بتاع اللي لازم احنا نتعرف
+
+282
+00:20:57,280 --> 00:21:01,180
+عليهو تصورة ان ال biochemical reaction مهم جدا في
+
+283
+00:21:01,180 --> 00:21:03,760
+عملية دي اللي هو التعرف و هتعرف عليها شغالة بشكل
+
+284
+00:21:03,760 --> 00:21:06,800
+جيد فين دي حاسبة immunological methods
+
+285
+00:21:06,800 --> 00:21:10,200
+immunological methods اللي هي سطر التالت
+
+286
+00:21:10,200 --> 00:21:15,240
+immunological methods وهي عبارة عن antigen
+
+287
+00:21:15,240 --> 00:21:18,960
+detection او antibody detection تركزوا معاه في
+
+288
+00:21:18,960 --> 00:21:22,900
+البسيطية دي لأنها كتير مهمة هناك فرق كبير بين ال
+
+289
+00:21:22,900 --> 00:21:28,310
+antigen و بين ال antibody detectionالان ال antigen
+
+290
+00:21:28,310 --> 00:21:32,470
+هو جزء من مين؟ من البكتيريا على اسم المثال ال
+
+291
+00:21:32,470 --> 00:21:37,630
+antibody هو ال response تبعنا للبكتيريا طيب طلعوا
+
+292
+00:21:37,630 --> 00:21:42,390
+على الحالة التلاتة البكتيريا لو مسكنها مباشرة في
+
+293
+00:21:42,390 --> 00:21:46,550
+العينة نفس البكتيريا بال culture صح ولا لأ؟ او بال
+
+294
+00:21:46,550 --> 00:21:50,950
+staining يبقى احنا قبضنا على الميزورين المتلبس
+
+295
+00:21:50,950 --> 00:21:57,280
+بذاته بذاته بدون اي اشاراتلكن الانتجين هو جزء من
+
+296
+00:21:57,280 --> 00:22:04,440
+البكتيريا و ليس البكتيريا الان الأسوأ من الجزء هو
+
+297
+00:22:04,440 --> 00:22:13,020
+الأثر واحد ارتكب جريمة قبل سنة وصار يريد في نفس
+
+298
+00:22:13,020 --> 00:22:19,300
+المكان اليوم كويس؟ إت المحقق و أخد بصمات من ضمن
+
+299
+00:22:19,300 --> 00:22:25,820
+البصمات أخد بصمات من؟ اللي قبل سنةفالان بيجيبوه
+
+300
+00:22:25,820 --> 00:22:29,380
+انت قتلت X اللي هو الجديد بيقولوا لا يا عم انا ما
+
+301
+00:22:29,380 --> 00:22:35,700
+قتلتش انا قتلت Y قبل سنة مش اليوم يبقى وجود أثر
+
+302
+00:22:35,700 --> 00:22:41,040
+للميكروب بشكل Antibody لا يعني وجود الميكروب اللي
+
+303
+00:22:41,040 --> 00:22:44,440
+قد يكون موجود في السابق وانا عارفين ان ال Antibody
+
+304
+00:22:44,440 --> 00:22:52,130
+أصلا انتج كاستجابة لل Antigen عشانيظل فيه أنه نوع
+
+305
+00:22:52,130 --> 00:22:56,470
+من المناعة ضد هذا المجرم، إذا ليس الكشف عن ال
+
+306
+00:22:56,470 --> 00:23:00,570
+antigen كالكشف عن مين؟ عن ال antibody أو عن
+
+307
+00:23:00,570 --> 00:23:04,430
+البكتيريا، مين أقوى حاجة أن نكشف البكتيريا؟ اللي
+
+308
+00:23:04,430 --> 00:23:09,730
+بعديها ال antigen و آخر حاجة مين؟ أنا لا .. دائما
+
+309
+00:23:09,730 --> 00:23:14,490
+و أبدا بقول لا أستطيع أن أقارن بين شغلتين، بين
+
+310
+00:23:14,490 --> 00:23:18,470
+فحصين موجودين و أقول هذا أحسن من هذاليش؟ لأنه كون
+
+311
+00:23:18,470 --> 00:23:22,130
+ال trend موجودين يبقى هذا له استخدامه و هذا له
+
+312
+00:23:22,130 --> 00:23:26,250
+استخدامه مش دايما بقدر أجي ال antigen فممكن أبحث
+
+313
+00:23:26,250 --> 00:23:29,890
+عن ال antibody في بعض الأحيان لكن بدا أكون فاهم
+
+314
+00:23:29,890 --> 00:23:34,390
+أنه قيمته لا تعني اللي هو وجود ال antigen أو تعني
+
+315
+00:23:34,390 --> 00:23:38,190
+وجود ال micro organism و عندي مجموعة احتمالات
+
+316
+00:23:38,190 --> 00:23:43,090
+هتكلم عنها لاحقا ان شاء الله اذا biochemical test
+
+317
+00:23:43,090 --> 00:23:51,060
+هتناخد قصةخدنا عينة من CSF عملنا لها streaking على
+
+318
+00:23:51,060 --> 00:23:56,460
+أكتر من وسط و طلعنا بكتيريا الواصف تبع المستعمرات
+
+319
+00:23:56,460 --> 00:23:58,620
+golden yellow pigmented فاكرين ال golden yellow
+
+320
+00:23:58,620 --> 00:24:03,020
+pigmented؟ على البلد أجار و عاملة beta hemolysis و
+
+321
+00:24:03,020 --> 00:24:06,780
+entire edge عملنا gram stain لجيناها gram postif
+
+322
+00:24:06,780 --> 00:24:10,440
+cocci in clusters عملتلها biochemical test كتاليز
+
+323
+00:24:10,440 --> 00:24:13,240
+test طلعت postif عملتلها coagulase طلعت postif
+
+324
+00:24:13,240 --> 00:24:19,020
+البكتيريا الأن استنفي ل focus areasعرفتها مش على
+
+325
+00:24:19,020 --> 00:24:26,420
+الوصف، وصف Gramstein و biochemical testبعض الأحيان
+
+326
+00:24:26,420 --> 00:24:31,860
+لا يكفي قصة جديدة عيد سطول زرعناه طلعت gram
+
+327
+00:24:31,860 --> 00:24:34,820
+negative بسلاي على ماكن كلاكتوز fermenter شكلها
+
+328
+00:24:34,820 --> 00:24:38,920
+pinkish colonies عملتلها biochemical test ان دول
+
+329
+00:24:38,920 --> 00:24:43,240
+test بُستف و بوكرسبلسكاور اه لأ احنا بيقولنا MR
+
+330
+00:24:43,240 --> 00:24:46,520
+بُستف و بوكرسبلسكاور negative citrate negative
+
+331
+00:24:46,520 --> 00:24:49,640
+يعني بُستف بُستف negative negative إشريش أكل يعني
+
+332
+00:24:49,640 --> 00:24:54,460
+طب وش و سوا كنا أنا في السطول إشريش أكل صح ولا لأ؟
+
+333
+00:24:54,580 --> 00:24:58,820
+يبقى كيف بدي أميز E.coli ممرضة وعن ال E.coli
+
+334
+00:24:58,820 --> 00:25:02,280
+الممرضة بدي أعمل serology الان بدي أشوف هل عندها
+
+335
+00:25:02,280 --> 00:25:07,000
+O157 الانتجين تبعها موجود ده ممرضة ولا لأ يبقى بعض
+
+336
+00:25:07,000 --> 00:25:09,400
+الأحيان اللي هيكفي ال biochemical identification
+
+337
+00:25:09,400 --> 00:25:14,720
+ونضطر لمين الآن لل serological identification طيب
+
+338
+00:25:15,470 --> 00:25:18,630
+زميلتكن تنبهت لقضية جالت الدكتورة ما هو مش كل ..
+
+339
+00:25:18,630 --> 00:25:22,290
+مش كل البكتيريا بتنسبق لـ gram stain و gram
+
+340
+00:25:22,290 --> 00:25:25,430
+positive و gram negative و قولت أنا كمان إضافة مش
+
+341
+00:25:25,430 --> 00:25:28,950
+كل البكتيريا أصلا نقدر ننميها و مش كل البكتيريا
+
+342
+00:25:28,950 --> 00:25:33,830
+بتنمو بشكل سريع زي ما كنا 24 ساعة عشان نقدر نحصل
+
+343
+00:25:33,830 --> 00:25:40,230
+على نتيجة تخلال 72 ساعة فهل هناك طرق لل rapid
+
+344
+00:25:40,230 --> 00:25:47,170
+diagnosis بدون مزرعة؟ نعمواحد بدنا نعرف في أي
+
+345
+00:25:47,170 --> 00:25:50,990
+حالات من الأول culture أو certain human pathogenes
+
+346
+00:25:50,990 --> 00:25:53,490
+including the causative agent of tuberculosis اللي
+
+347
+00:25:53,490 --> 00:25:56,070
+هي ال micro bacterium tuberculosis ال lime disease
+
+348
+00:25:56,070 --> 00:25:58,410
+اللي هي ال borrelia و ال syphilis اللي هي
+
+349
+00:25:58,410 --> 00:26:02,070
+tryponema pallidium either cannot be isolated إما
+
+350
+00:26:02,070 --> 00:26:08,790
+إنها مابنجرش نفسها أساسا أو إنه slow growth طب هذه
+
+351
+00:26:08,790 --> 00:26:14,550
+مشكلةالتربونية بلدهم لحد الآن لم تعزل على
+
+352
+00:26:14,550 --> 00:26:18,230
+artificial medium الميكروباكتيروم تبركلوسس بتقعد
+
+353
+00:26:18,230 --> 00:26:24,110
+ست تسابيع البوريليا ممكن تقعد أسبوع كلهم فيهم
+
+354
+00:26:24,110 --> 00:26:29,910
+مشكلة يا بطيئة يا ما تنموش فبالتالي
+
+355
+00:26:29,910 --> 00:26:32,670
+ال successful isolation في الفترة التانية غالبا
+
+356
+00:26:32,670 --> 00:26:36,570
+بيكون اما سلوك وبعض الأحيان impossible مستحيل
+
+357
+00:26:38,160 --> 00:26:40,700
+بالحالة direct detection of bacteria without
+
+358
+00:26:40,700 --> 00:26:45,380
+culture is possible in some cases اعطيكم مثال لو
+
+359
+00:26:45,380 --> 00:26:51,240
+احنا أخدنا عين تسبيوتام احنا بيقولنا المريض ال
+
+360
+00:26:51,240 --> 00:26:54,140
+tuberculosis هو يبقى او غالبا palmonary
+
+361
+00:26:54,140 --> 00:26:58,200
+tuberculosis فبيكون فيه اللي هو ال .. ال .. ال ..
+
+362
+00:26:58,200 --> 00:26:58,580
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+363
+00:26:58,580 --> 00:26:58,780
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+364
+00:26:58,780 --> 00:26:59,060
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+365
+00:26:59,060 --> 00:26:59,260
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+366
+00:26:59,260 --> 00:26:59,580
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+367
+00:26:59,580 --> 00:26:59,600
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+368
+00:26:59,600 --> 00:27:00,100
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+369
+00:27:00,100 --> 00:27:00,220
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+370
+00:27:00,220 --> 00:27:04,100
+ال .. ال .. ال .. ال ..مش درستوا انتوا acid fast
+
+371
+00:27:04,100 --> 00:27:07,220
+stain؟ طب لو عملنا ال dispute ال acid fast stain
+
+372
+00:27:07,220 --> 00:27:11,340
+مش خلال نصف ساعة بنقدر نشوف برضه ال acid fast بس
+
+373
+00:27:11,340 --> 00:27:14,480
+ال lie of presence or absence بنقدر من درج بنقدر
+
+374
+00:27:14,480 --> 00:27:17,200
+هذا rapid .. rapid approach ولا مش rapid approach؟
+
+375
+00:27:17,200 --> 00:27:20,980
+Rapid approach خدوا بالكوا نفس ال rapid approach
+
+376
+00:27:20,980 --> 00:27:23,780
+اللي هو ال staining بال acid fast في كمان staining
+
+377
+00:27:23,780 --> 00:27:27,680
+تاني ما أذكر معاكوا ولا درستوا في المعمل اسمه
+
+378
+00:27:27,680 --> 00:27:31,490
+fluorescent staining fluorescent stainو ماذا انا
+
+379
+00:27:31,490 --> 00:27:33,550
+اقصد ب fluorescence studying لإنه كتير concept مهم
+
+380
+00:27:33,550 --> 00:27:36,030
+اسمه مش بدكورة معانا بس انا متأكد هتمور معانا
+
+381
+00:27:36,030 --> 00:27:39,910
+somewhere فيرد ان تبقى لها كويس و انا هشرع على
+
+382
+00:27:39,910 --> 00:27:44,030
+الصبورة بعض ال .. ال .. الاشياء المتعلقة بال
+
+383
+00:27:44,030 --> 00:27:48,730
+fluorescence عشان تكون الصورة أوضح ان شاء الله
+
+384
+00:27:48,730 --> 00:27:53,790
+بالنسبة لنا هتبقى اقولنا انه مادة ال fluorescence
+
+385
+00:27:53,790 --> 00:27:57,910
+هي substance لو ضربناها بال UV كلمة ضربناها يعني
+
+386
+00:27:57,910 --> 00:28:02,050
+اذا زرق عليها شعرit will absorb الآن ال absorption
+
+387
+00:28:02,050 --> 00:28:05,450
+سباق ال UV بيعمل excitation لل fluorescent
+
+388
+00:28:05,450 --> 00:28:11,610
+substance وبيعمل emitting لvisible light وكلمة
+
+389
+00:28:11,610 --> 00:28:14,890
+visible light هي ضوء مرقى والضوء المرقى هنا غالبا
+
+390
+00:28:14,890 --> 00:28:19,050
+بيكون colored colored يعني أما لون أصفر أو أخضر أو
+
+391
+00:28:19,050 --> 00:28:22,130
+أزرق أو أحمر يعني بيختلف من حسب نوع مين ال
+
+392
+00:28:22,130 --> 00:28:28,430
+fluorescent substance لو علمتمأن هناك صبغة بترتبط
+
+393
+00:28:28,430 --> 00:28:31,970
+specifically بال mycolic acid ايش هو ال mycolic
+
+394
+00:28:31,970 --> 00:28:39,690
+acid؟ ال mycolic acid هو ال واكسي لبت اللي موجود
+
+395
+00:28:39,690 --> 00:28:44,630
+في ال mycobacterium تبركيلووسس اسم هذه البكتيريا
+
+396
+00:28:44,630 --> 00:28:53,030
+هي ال mycolic acid اسم
+
+397
+00:28:53,030 --> 00:28:58,220
+المادة Rodamineو في مادة أخرى اسمها Oramine
+
+398
+00:28:58,220 --> 00:29:01,760
+Oramine
+
+399
+00:29:01,760 --> 00:29:08,020
+O و Rodamine أين منهما أو كلهما بيتطابط
+
+400
+00:29:08,020 --> 00:29:13,580
+Specifically مع ميجولك أسد لو صبغنا عيلة شريحة
+
+401
+00:29:13,580 --> 00:29:20,140
+فيها fixed smear من اللي هو ال .. ال .. ال .. ال
+
+402
+00:29:20,140 --> 00:29:20,200
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+403
+00:29:20,200 --> 00:29:20,360
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+404
+00:29:20,360 --> 00:29:20,640
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+405
+00:29:20,640 --> 00:29:21,260
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+406
+00:29:21,260 --> 00:29:22,140
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+407
+00:29:22,140 --> 00:29:22,900
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+408
+00:29:22,900 --> 00:29:22,900
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+409
+00:29:22,900 --> 00:29:22,920
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+410
+00:29:22,920 --> 00:29:22,920
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+411
+00:29:26,030 --> 00:29:28,430
+حايل .. حايل .. حايل .. حايل سبقها وكونوا
+
+412
+00:29:28,430 --> 00:29:31,210
+specifically في الميكوليك أسد يبجلان يسبق
+
+413
+00:29:31,210 --> 00:29:35,170
+البكتيريا الأخرى فقط من؟ الميكو بكتيريا بعدها نعمل
+
+414
+00:29:35,170 --> 00:29:39,510
+عملية .. عملية غسيل و بنطفي في مادة اسمها بُتاسيم
+
+415
+00:29:39,510 --> 00:29:42,370
+بالمنجليات بنخفف من ال fluorescence اللي تبقى
+
+416
+00:29:42,370 --> 00:29:45,750
+الصبغة و بتنطلع تحت الميكروسكوب المرة هذه بمعناه
+
+417
+00:29:45,750 --> 00:29:50,130
+أسهل detection هتشوفوه في حياتكم هو الميكو بكتيريا
+
+418
+00:29:50,130 --> 00:29:53,310
+متبركوليستيكيا على ال high power تعرف ال high
+
+419
+00:29:53,310 --> 00:29:53,690
+power؟
+
+420
+00:29:56,700 --> 00:30:00,340
+مش ال oil، ال oil immersion مية، مظبوط؟ يعني على
+
+421
+00:30:00,340 --> 00:30:04,120
+أربعين اكس ممكن نعمل detection لمين لاللي هو ال
+
+422
+00:30:04,120 --> 00:30:07,860
+micro bacterium tuberculosis، إذا هي تقنية برضه
+
+423
+00:30:07,860 --> 00:30:13,540
+بال staining و rapid و rapid و سريعة و easyو للأسف
+
+424
+00:30:13,540 --> 00:30:16,880
+لحد الآن لم تطبقوا .. يمكن السنة هذه تطبقوها في ..
+
+425
+00:30:16,880 --> 00:30:20,480
+في أحد العيادات في قطاع غزة لكن احنا كجامعة في
+
+426
+00:30:20,480 --> 00:30:24,300
+المختبر الصحيح عملناها يمكن قبل خمساش سنة و ورناهم
+
+427
+00:30:24,300 --> 00:30:27,780
+نتعيش هنا و نالهم هاي الصبغة اتفضلوا هدية gift بس
+
+428
+00:30:27,780 --> 00:30:32,280
+انتوا عملوا الفحوصات لكن تعقيدات الإدارية غالبا
+
+429
+00:30:32,280 --> 00:30:38,820
+بتكون معينةاحنا نسميها تعقيدات إدارية، عشانك مش
+
+430
+00:30:38,820 --> 00:30:41,980
+جادين نفسرها لإيش يعني، نفسيات الناس، يعني كيف
+
+431
+00:30:41,980 --> 00:30:46,140
+تشتغل مبنها طيب،
+
+432
+00:30:46,140 --> 00:30:51,760
+فين دي هنا أحد الطرق للكشف عن الانتيجنات؟ يمكن هنا
+
+433
+00:30:51,760 --> 00:30:56,240
+الأطفال أكثر فئة بتاعي من التنسوليتس أو إتهاب
+
+434
+00:30:56,240 --> 00:31:01,940
+الحلقوالمسبب الأكبر .. المسبب الأكبر لانتهابات
+
+435
+00:31:01,940 --> 00:31:06,220
+الحلق هو ال .. ال bacterial cause هو ال .. the
+
+436
+00:31:06,220 --> 00:31:10,660
+streptococcus biogenes طيب أنا أقعد تلت أيام
+
+437
+00:31:10,660 --> 00:31:14,720
+culture .. تلت أيام culture كاملة من ألفها إلي آخر
+
+438
+00:31:14,720 --> 00:31:19,780
+تقريبا هذا ال minimum 72 ساعة و أترك المريض بدون
+
+439
+00:31:19,780 --> 00:31:24,240
+علاج طول الفترة هذه الصحيح بتكون الأمر مزعجفبناخد
+
+440
+00:31:24,240 --> 00:31:29,060
+عينة من المريض مسحة ب cotton swab شايفين الإشارة
+
+441
+00:31:29,060 --> 00:31:32,560
+اللي هنا عبارة
+
+442
+00:31:32,560 --> 00:31:37,700
+عن عوض رفيق على طرفه قطعة ثميقة من ال cotton
+
+443
+00:31:37,700 --> 00:31:44,320
+ملفوفة بطريقة صلبة بنعمل illumination جيد للفم و
+
+444
+00:31:44,320 --> 00:31:47,960
+بنضغط على اللسان بخافض لللسان يسموه tongue
+
+445
+00:31:47,960 --> 00:31:51,300
+depressor و بنعمل كلمة مرة illumination مرة أخر في
+
+446
+00:31:51,300 --> 00:31:57,300
+ضوء بيضوي الفموبنمرر ال .. ال .. السواق دون أن
+
+447
+00:31:57,300 --> 00:32:01,640
+نلمس أي مكان آخر في فم لأن الفم عشرة عشرة أس تسعة
+
+448
+00:32:01,640 --> 00:32:05,060
+عشرة أس اتناش من البكتيريا فنمنع نلمس أي مكان سواء
+
+449
+00:32:05,060 --> 00:32:08,680
+من التونسل و بنضغط على التونسل و بنلف و بنطلع
+
+450
+00:32:08,680 --> 00:32:17,920
+بسرعة دون أن نلمس أخدنا مسحة من التونسل بنجص قطعة
+
+451
+00:32:17,920 --> 00:32:21,120
+القطن و بنحطها في سائل لل extraction تبعي ال
+
+452
+00:32:21,120 --> 00:32:24,950
+antigen بيدوب البكتيريابطلنا مين الآن؟ ال antigen
+
+453
+00:32:24,950 --> 00:32:29,430
+خدوا بالكوا ال antigen الآن هو عبارة عن fragment
+
+454
+00:32:29,430 --> 00:32:35,770
+من البكتيريا و ال antibody أصغر أصلا من البكتيريا
+
+455
+00:32:35,770 --> 00:32:40,870
+فارتباط صغير مع صغير مش هنشوفه،
+
+456
+00:32:40,870 --> 00:32:46,010
+بدنا carrierبروحوا لما بيعطونا ال antibody ك
+
+457
+00:32:46,010 --> 00:32:48,490
+reagent اللي بيقونا إياه في الشركات، بيعطونا مش
+
+458
+00:32:48,490 --> 00:32:52,470
+antibody هيك لحاله، بيعطونا antibody مركّب على ..
+
+459
+00:32:52,470 --> 00:32:56,170
+مركّب على latex beads أو particles، إيش هم ال
+
+460
+00:32:56,170 --> 00:33:01,670
+latex دولة؟ عبارة عن كوريات من ال plastic صغيرة
+
+461
+00:33:01,670 --> 00:33:06,370
+تشبه في حجمها ال RBCs، بتعرف ال RBCs؟ بتعرف حجم ال
+
+462
+00:33:06,370 --> 00:33:14,290
+RBCs؟7.5 micron فاحنا بنركب ال antibody على ال
+
+463
+00:33:14,290 --> 00:33:19,950
+latex هذه عشان هو مالوش اي فايد اخدوا بالكوا
+
+464
+00:33:19,950 --> 00:33:23,530
+الفايد هو انه to visualize the reaction جعل
+
+465
+00:33:23,530 --> 00:33:27,090
+التفاعل اللي سمنا agglutination اللي درستوه في
+
+466
+00:33:27,090 --> 00:33:29,850
+أحياء اللي درستوه في أحياء اللي هو لما عملتوا
+
+467
+00:33:29,850 --> 00:33:35,940
+حصائل الدمفصار لدن هو يبقى عن أنتجين ال antibody
+
+468
+00:33:35,940 --> 00:33:38,720
+إذا توافق ال أنتجين مع ال antibody بيعمل
+
+469
+00:33:38,720 --> 00:33:42,940
+agglutination صح؟ clumping فهذا اسمه agglutination
+
+470
+00:33:42,940 --> 00:33:47,140
+reaction عن مين كشفنا احنا؟ كشفنا عن ال antigen
+
+471
+00:33:47,140 --> 00:33:50,680
+تبع البكتيريا اللي اسمها streptococcus biogenes
+
+472
+00:33:50,680 --> 00:33:54,680
+إذا ال antigen موجود يبقى البكتيريا موجود غير عن
+
+473
+00:33:54,680 --> 00:33:58,640
+ال antibody و إن ال antibody موجود يمكن البكتيريا
+
+474
+00:33:58,640 --> 00:34:05,960
+كانت موجودة زمان صح؟وغان منه لم يصب بالتنسولايتس
+
+475
+00:34:05,960 --> 00:34:10,040
+فلو أنا أخدت منكوا أينات الآن من الدم وفحصت عن ال
+
+476
+00:34:10,040 --> 00:34:15,600
+antibody معظمكوا حالجي boosted لأن
+
+477
+00:34:15,600 --> 00:34:19,280
+ال antibody موجود، بس مش من ال infection حالية، من
+
+478
+00:34:19,280 --> 00:34:22,980
+ال infection السادة، هاي اختلاف بين كلمة antigen
+
+479
+00:34:22,980 --> 00:34:26,520
+وكلمة antibody في هذه الحالة إن وجود ال antigen هو
+
+480
+00:34:26,520 --> 00:34:28,780
+مؤشر لوجود ال microbe نفسه
+
+481
+00:34:31,940 --> 00:34:35,320
+طيب من التقنيات الحديثة في ال rapid diagnosis حاجة
+
+482
+00:34:35,320 --> 00:34:40,760
+اسمها ال bacterial DNA sequences الآن
+
+483
+00:34:40,760 --> 00:34:43,480
+البكتيريا بتختلف عن بعضها و عشانك أعطانا أسماء
+
+484
+00:34:43,480 --> 00:34:47,900
+مختلفة ما الذي يجعل البكتيريا X مختلفة عن Y؟ مش
+
+485
+00:34:47,900 --> 00:34:53,920
+مادتها ورعاتية الهوية الأدق إن في التعرف هي المادة
+
+486
+00:34:53,920 --> 00:35:00,560
+الورعاتية ال Lكل الميكروبات الطبية تقريبا بنسبة 99
+
+487
+00:35:00,560 --> 00:35:05,340
+% كل العلماء أولدوا لها ما يسمى ب unique sequence
+
+488
+00:35:05,340 --> 00:35:10,640
+كلمة unique يعني خريط، مميز، هذا ال sequence مميز
+
+489
+00:35:10,640 --> 00:35:15,260
+البكتيريا هذه عن البكتيريا الأخرى وبالتالي طالع
+
+490
+00:35:15,260 --> 00:35:18,580
+بعيد ال unique sequence لكل موضوع نوع البكتيريا
+
+491
+00:35:18,580 --> 00:35:20,880
+المعروف يبقى ان انا بقدر اشتغل عليها
+
+492
+00:35:20,880 --> 00:35:27,550
+identification كانت بتواجهين مشكلةإنه كيف بدي أكشف
+
+493
+00:35:27,550 --> 00:35:32,730
+عن مادة و .. عن مادة وراتية لميكروب واحد كمية
+
+494
+00:35:32,730 --> 00:35:38,290
+قليلة يصعب الكاشف دخل نظام ال PCR اللي هو
+
+495
+00:35:38,290 --> 00:35:41,870
+Polymerase Chain Reaction وحل هذا المشكلة قاللي
+
+496
+00:35:41,870 --> 00:35:46,070
+إذا أنت عندك one copy one copy من ال DNA اللي بدك
+
+497
+00:35:46,070 --> 00:35:50,030
+إياه أنا بخليلك إياه خلال خمس ساعات، ست ساعات،
+
+498
+00:35:50,030 --> 00:35:52,610
+بخليك إياه مليارات من ال copies كيف؟
+
+499
+00:35:54,770 --> 00:35:58,630
+عنا هاي المادة الوراثية خلينا على اللوحة طول
+
+500
+00:35:58,630 --> 00:36:03,450
+الفترة ان شاء الله هاي المادة الوراثية القطعة
+
+501
+00:36:03,450 --> 00:36:06,870
+الصغيرة هادي هي الـ Nucleotide الـ Nucleotidات
+
+502
+00:36:06,870 --> 00:36:10,410
+تمام؟ و القطعة الحمراء هادي بيسموها Primers
+
+503
+00:36:10,410 --> 00:36:13,950
+Primers هي عبارة عن Sequence Complementary خدوا
+
+504
+00:36:13,950 --> 00:36:18,210
+بالكوا، ده اللي ماأمرش أعرفه ال PCR أنا عارف ال
+
+505
+00:36:18,210 --> 00:36:23,420
+sequence تبع المادة اللي بديها، مظبوط؟ عارففبقى
+
+506
+00:36:23,420 --> 00:36:29,020
+بصمم قطعة صغيرة من complementary DNA لـ 3 prime
+
+507
+00:36:29,020 --> 00:36:32,360
+المنطقة هذه و لـ 3 prime المنطقة هذه هذا بيسميه
+
+508
+00:36:32,360 --> 00:36:36,060
+Primer ليش ال Primer المهم؟ بدونه ال RNA
+
+509
+00:36:36,060 --> 00:36:39,580
+Polymerase .. عفوا ال DNA Polymerase مابيشتغلش
+
+510
+00:36:39,580 --> 00:36:46,580
+الآن أول خطوة عملناdenaturation فسخنا اللي هم
+
+511
+00:36:46,580 --> 00:36:50,320
+المادة اللي وراتيها عن بعضها من بعض بعدين عملنا ما
+
+512
+00:36:50,320 --> 00:36:53,720
+يسمى ب ال primer annealing تمام اللي يسميه
+
+513
+00:36:53,720 --> 00:36:57,880
+annealing و لحظة العملية بتتم عليها على different
+
+514
+00:36:57,880 --> 00:37:02,400
+temperatures بعد ال primer annealing اتى ال DNA
+
+515
+00:37:02,400 --> 00:37:08,460
+polymerase وكمل اللي هو ال elongation process يعني
+
+516
+00:37:08,460 --> 00:37:09,760
+كمل القطعة
+
+517
+00:37:15,950 --> 00:37:20,190
+لو قدنا التلات خطوات مرة أخرى أكم قطعة سياران دي؟
+
+518
+00:37:20,190 --> 00:37:24,730
+أربعة لإنه في عندي واحدة صارت تنتين، تنتين صاروا
+
+519
+00:37:24,730 --> 00:37:30,550
+أيه؟ أربعة فاكرين السيارة اللي هي السيارة اللي
+
+520
+00:37:30,550 --> 00:37:33,530
+كنتوا هتشتروها و تنغشوا في الموضوع و تدفعوا فلوس
+
+521
+00:37:33,530 --> 00:37:36,410
+كتير في هذا الموضوع اتنين، أربعة، تمانين، ستاشرين،
+
+522
+00:37:36,410 --> 00:37:39,850
+تلاتين، أربعة و ستين إلى أرقام ضخمة جدا خلال
+
+523
+00:37:39,850 --> 00:37:44,410
+within hours و طبعا اليوم بجانا زمان نمسك الأنبوبة
+
+524
+00:37:45,350 --> 00:37:49,350
+و نسخنها و نبردها اليوم فيه جهاز صغير هلجد اسمه
+
+525
+00:37:49,350 --> 00:37:53,230
+thermal cycler بيجيهم بالمهمة و بنبرمج و احنا برد
+
+526
+00:37:53,230 --> 00:37:58,370
+سخن عسب المادة اللي بدنايها و بكل سهولة بيعملنا ال
+
+527
+00:37:58,370 --> 00:38:03,610
+cycle طبعا هدا عمل detection هو ماعملش detectionهو
+
+528
+00:38:03,610 --> 00:38:07,390
+على حاجة واحدة amplification القطعة الواحدة أو
+
+529
+00:38:07,390 --> 00:38:11,690
+القطعة الصغيرة خلّاها قطعة كبيرة لكن سهل علينا
+
+530
+00:38:11,690 --> 00:38:15,450
+عملية ال detection لما يكون قطعة كبيرة يكون ال
+
+531
+00:38:15,450 --> 00:38:19,530
+detection سهل طرق ال detection عاد مختلفة ومتنوعة
+
+532
+00:38:23,720 --> 00:38:26,900
+ك .. ك method سريعة فاكرين ان احنا قولنا قبل شوية
+
+533
+00:38:26,900 --> 00:38:31,380
+ال acid fast stain could be used لمين؟ ل .. ل .. ل
+
+534
+00:38:31,380 --> 00:38:34,100
+.. ل ال mycobacterium tuberculosis او حتى ل ال
+
+535
+00:38:34,100 --> 00:38:38,000
+mycobacterium library اللي هي ال U ده أساسا الفحص
+
+536
+00:38:38,000 --> 00:38:41,680
+الأساسي الوحيد لل U ده هو ال acid fast stain فلابد
+
+537
+00:38:41,680 --> 00:38:45,300
+method هو كده والصورة لا .. لا أدري إن كانت كتير
+
+538
+00:38:45,300 --> 00:38:51,090
+واضحة لكن ها لإنها اللي هو البسلية شايفينها؟طبعا
+
+539
+00:38:51,090 --> 00:38:53,890
+هي بتوصف ال .. ال .. ال acid phosphate بصراحة انها
+
+540
+00:38:53,890 --> 00:38:58,090
+thin very thin ان هذا الصورة مكبر اكتر من الصيانات
+
+541
+00:38:58,090 --> 00:39:00,390
+تحت ال microscope بتشوفها اشهر جدا بيشوفوها اصغر
+
+542
+00:39:00,390 --> 00:39:05,810
+شوية احنا
+
+543
+00:39:05,810 --> 00:39:09,650
+أخدنا serology و عملنا detection ل antigen من ال
+
+544
+00:39:09,650 --> 00:39:15,230
+throat swab صح؟ الان ممكن ناخد عين الدم عين الدم و
+
+545
+00:39:15,230 --> 00:39:20,190
+ناخد منها ال serumوفي السيرام بندور على إيش؟ بندور
+
+546
+00:39:20,190 --> 00:39:23,930
+على ال antibody و أعطيكم مثال على مرض بتشيخ قصفيات
+
+547
+00:39:23,930 --> 00:39:30,730
+بطريقة تعرفوا ريكيزيا؟ الريكيزيا انت بعد ما تقولوا
+
+548
+00:39:30,730 --> 00:39:34,270
+ريكيزيا ده بتقولوا على رأيك كلاميديا لإنه تنتن هو
+
+549
+00:39:34,270 --> 00:39:37,450
+plant intracellular برضها الريكيزيا بتسبب بعض
+
+550
+00:39:37,450 --> 00:39:39,770
+الأمراض من ضمن الأمراض الحالي بيسموها الtyphus
+
+551
+00:39:39,770 --> 00:39:45,300
+fever مش typhoid الtyphus هم الtyphusفي غزة طريقة
+
+552
+00:39:45,300 --> 00:39:53,100
+التشخيص الوحيدة لحمة Tefas هو فحص اسمه Ox-19 Ox-19
+
+553
+00:39:53,100 --> 00:39:58,380
+عبارة عن أنتجين بنكشف فيه عن ال antibody في دم
+
+554
+00:39:58,380 --> 00:40:03,000
+اللي هو المرض، هاي التشخيص المتاح الوحيد في قطاع
+
+555
+00:40:03,000 --> 00:40:09,260
+غزة، امام؟ هتقولولي ان ال antibody يمكن تكون من
+
+556
+00:40:09,260 --> 00:40:12,720
+إصابة سابقة إذا الفحص طلع بوسطه، صح؟ يمكن كلها
+
+557
+00:40:12,720 --> 00:40:19,850
+بوسعيةويمكن من إصابة حاليا مين اللي بيقرر انا بعمل
+
+558
+00:40:19,850 --> 00:40:23,230
+test و اعمل deduction و باللي بيقرر مين الطبيب
+
+559
+00:40:23,230 --> 00:40:27,110
+based on the clinical picture بتكونش تنص في يوم من
+
+560
+00:40:27,110 --> 00:40:33,350
+الأيام انه الطبيب حادق و عنده معلومات من خلالها مش
+
+561
+00:40:33,350 --> 00:40:37,230
+بس من .. يعني هو بيستخدم خدماتك diagnostic
+
+562
+00:40:37,230 --> 00:40:42,480
+services للمساعدة في التشخيص و ليس أداة رئيسيةفي
+
+563
+00:40:42,480 --> 00:40:45,900
+التشخيص وبالتالي هو يمتل ال clinical picture صورة
+
+564
+00:40:45,900 --> 00:40:49,240
+سريرية عن المريض قد تساعده لما بيعمل differential
+
+565
+00:40:49,240 --> 00:40:52,460
+diagnosis بيقول المرض هدا احتمال X او Y تعالى
+
+566
+00:40:52,460 --> 00:40:58,120
+نعمله فحص فاحنا بنساعده بنرجحله بعض اللي هو العمل
+
+567
+00:40:58,120 --> 00:41:03,430
+بنأكل معلومات واحدةعشانك انا بقول دايما و ابدا
+
+568
+00:41:03,430 --> 00:41:09,910
+احنا ليسنا في مجال التشخيص احنا في مجال مساعدة في
+
+569
+00:41:09,910 --> 00:41:13,610
+التشخيص وبتالي اياك انك في يوم من الأيام تتجرأ
+
+570
+00:41:13,610 --> 00:41:17,910
+وتعطي نتائج او تناقش نتائج مع المرض طبعا انا
+
+571
+00:41:17,910 --> 00:41:22,430
+بقولكوا الأمر مش سهل احنا مجتمعنا بيدغط على فني
+
+572
+00:41:22,430 --> 00:41:25,250
+المقتبر بيدغط على فني المقتبر او الخصائص التحيات
+
+573
+00:41:25,250 --> 00:41:29,690
+الطبية ان يفسرله النتائج وانا عملت في مجال التحيات
+
+574
+00:41:29,690 --> 00:41:34,040
+الطبية لفترةوكنت اواجه بعض ال .. ال .. ال .. يعني
+
+575
+00:41:34,040 --> 00:41:37,060
+الضغوطات من الناس لكن كنا ماخدين قرار في المكان
+
+576
+00:41:37,060 --> 00:41:40,700
+اللي بنشتغل فيه ممنوع مناقشة نتائج مع المرضى، فكان
+
+577
+00:41:40,700 --> 00:41:44,740
+افضل وسيلة انه ال .. النتائج تغلق في الظرف
+
+578
+00:41:47,560 --> 00:41:51,700
+يفتح بعض .. بعض منهم يفتح الضغط يقرأ يرجع طب بس
+
+579
+00:41:51,700 --> 00:41:55,260
+قوللي إيش فيه مابيش فيه احنا مابنفهم في تفسير
+
+580
+00:41:55,260 --> 00:41:58,920
+النتائج .. تفسير النتائج على مين على الطبيعة طبعا
+
+581
+00:41:58,920 --> 00:42:03,140
+الضغوطات هنا في المجتمع بتولد من إنه يعني أنت دارس
+
+582
+00:42:03,140 --> 00:42:07,460
+أربع سنوات أو خمس سنوات و مابتعرفش أه مابعرفش مافي
+
+583
+00:42:07,460 --> 00:42:11,620
+.. it is easy to say مابعرفش بأدب طبعا واحترام لكن
+
+584
+00:42:11,620 --> 00:42:15,840
+في الآخر بدنا نعرف إنه أي كلام ممكن نقوله احنا
+
+585
+00:42:15,840 --> 00:42:21,230
+ممكن يؤدي إلىيعني اتجاهات متنوعة، اعطيكوا مثل،
+
+586
+00:42:21,230 --> 00:42:25,830
+فحصك نظيف، لو قلنا للمريض فحصك نظيف، ايه مفهوم من
+
+587
+00:42:25,830 --> 00:42:29,130
+نظيف يعني؟ ان هو خالي، هو اصلا هذا الفحص هو اللي
+
+588
+00:42:29,130 --> 00:42:31,530
+بتشخصه يا إما عيال يا إما مش عيال، يمكن مايرجعك
+
+589
+00:42:31,530 --> 00:42:35,350
+للطبيب و يروح على البيت، مايرجعك للطبيب، فحصك
+
+590
+00:42:35,350 --> 00:42:38,730
+negative، فحصك negative، لازم الطبيب يعرف إيش
+
+591
+00:42:38,730 --> 00:42:39,690
+المشكلة اللي عندك
+
+592
+00:42:42,600 --> 00:42:46,420
+وبالتالي حديثك مع المريض قد يؤذي عملية التشخيص
+
+593
+00:42:46,420 --> 00:42:49,600
+وبالتالي نرجو من حضراتكم إنكم من الأن تاخدوا قرار
+
+594
+00:42:49,600 --> 00:42:52,000
+وعادة لنفسكم إنكم ما تناقشوا
+
+595
+00:42:56,090 --> 00:42:59,150
+طيب احنا كنا .. احنا بدأنا بالعلاج اللي قلنا في ال
+
+596
+00:42:59,150 --> 00:43:03,850
+profilaxis لكن لا بأس ان يكون لنا فكرة كيف او ايش
+
+597
+00:43:03,850 --> 00:43:07,810
+هي التقنيات المستخدمة في ال profilaxis profilaxis
+
+598
+00:43:07,810 --> 00:43:12,430
+يعني في انا قائمة من الأمراض بيسموها vaccine
+
+599
+00:43:12,430 --> 00:43:16,690
+preventable diseases الأمراض اللتي يمكن منعها بال
+
+600
+00:43:16,690 --> 00:43:21,480
+vaccinationعن ان ناخد أمثلة تيتانوس، Pertosis،
+
+601
+00:43:21,480 --> 00:43:25,620
+Diphtheria، Measles، Rubella، هد أسمك و لأ الدولة
+
+602
+00:43:25,620 --> 00:43:28,780
+دفتر التطيب تبعك و اللي صغير، اللي و أنتوا صغار،
+
+603
+00:43:28,780 --> 00:43:32,100
+هتلاجوا مجموعة كبيرة من الأمراض البكتيرية و
+
+604
+00:43:32,100 --> 00:43:36,920
+البيروسية مُنِعت تقريبا أو مُنِع درارها اللي كان
+
+605
+00:43:36,920 --> 00:43:41,220
+يقتل بنسب كبيرة مُنِع درارها تقريبا و أصبح إلى ..
+
+606
+00:43:41,220 --> 00:43:43,460
+يعني في البعض .. نشير الأطفال تقريبا في فلسطين
+
+607
+00:43:43,460 --> 00:43:48,240
+Zero من التمانينات، القرن الماضيالـ Tetanus
+
+608
+00:43:48,240 --> 00:43:52,760
+تقريباً Zero يعني فينا أموات كثيرة بالفعل انخفضت
+
+609
+00:43:52,760 --> 00:43:56,920
+مستوياتها إلى الصفر بفعل ال vaccination أنواع ال
+
+610
+00:43:56,920 --> 00:44:00,420
+vaccine inactivated vaccines يعني تحتوي على
+
+611
+00:44:00,420 --> 00:44:06,760
+بكتيريا ميتة أو فيروسات ميتة attenuated يعني مضعفة
+
+612
+00:44:06,760 --> 00:44:11,080
+يعني بكتيريا عرضناها لإجراءات جعلناها غير قادرة
+
+613
+00:44:11,080 --> 00:44:18,060
+على إحداث المرض لكنهذا .. هذا .. هذا أحد عيوبه أنه
+
+614
+00:44:18,060 --> 00:44:21,940
+ممكن البكينة الضعيفة إذا لقت البيئة المناسبة إنها
+
+615
+00:44:21,940 --> 00:44:27,180
+ت .. ترجع لشراسة تبعتها التوكسويد العظيم اللي احنا
+
+616
+00:44:27,180 --> 00:44:31,840
+ذكرناه لكوا في ال Exoxin اللي حاولنا إنه نشيل من
+
+617
+00:44:31,840 --> 00:44:38,320
+صفة ال toxicity و نفلي ال antigen أحلى حاجة في هذا
+
+618
+00:44:38,320 --> 00:44:43,530
+ال vaccine الأخير هذا أو الرابع ال auto vaccineA
+
+619
+00:44:43,530 --> 00:44:45,550
+vaccine prepared from cultures of organisms
+
+620
+00:44:45,550 --> 00:44:48,210
+isolated from patients on tissue or secretions
+
+621
+00:44:48,210 --> 00:44:51,890
+يعني أخدنا البكتيريا اللي أعدلناها من فلان
+
+622
+00:44:51,890 --> 00:44:58,450
+وعلجناها إما قتلناها أو ضعفناها و رجعناها للمرض
+
+623
+00:44:58,450 --> 00:45:02,170
+Auto Vaccine الأخير اللي هو ال polysaccharide
+
+624
+00:45:02,170 --> 00:45:05,710
+vaccine و احنا نتكلم في غالب الأمر عن اللي هو ال
+
+625
+00:45:05,710 --> 00:45:09,690
+capsular polysaccharide في الغالب أعطيكم مثل على
+
+626
+00:45:09,690 --> 00:45:13,910
+capsular polysaccharideمهم جدا وشهير جدا اللى هو
+
+627
+00:45:13,910 --> 00:45:19,350
+ال capsule تبعت ال nyseria meningitis و ال capsule
+
+628
+00:45:19,350 --> 00:45:22,790
+تبعت ال streptococcus pneumonia و ال capsule تبعت
+
+629
+00:45:22,790 --> 00:45:25,950
+ال hemophilus influenza هدول أخدوا بالكوا هتلاقوهم
+
+630
+00:45:25,950 --> 00:45:33,110
+بس أنا بتكررهم مع بعض كتير هحكيهم كمان مرة نيسيريا
+
+631
+00:45:33,110 --> 00:45:37,090
+مينيجدايدس streptococcus pneumonia
+
+632
+00:45:39,960 --> 00:45:46,660
+يمو فلس انفلونزا وخميرة
+
+633
+00:45:46,660 --> 00:45:54,080
+اسمها Cryptococcus ايش معنى هدول الأربع؟ هدول نجوم
+
+634
+00:45:54,080 --> 00:45:58,860
+نجوم في عالم meningitis يعني بسبب meningitis بشكل
+
+635
+00:45:58,860 --> 00:46:07,370
+شائع كلهم بيتميزوا أنهم بوريسا كرايد كابسولاللي هم
+
+636
+00:46:07,370 --> 00:46:11,550
+polysaccharide capsule بنستخدم ال capsule تبعتهم
+
+637
+00:46:11,550 --> 00:46:15,730
+في شغلتين في ال diagnosis نكشف عنها ك antigen و
+
+638
+00:46:15,730 --> 00:46:18,590
+بنستخدمها كمان في ال vaccine preparation بنحضر
+
+639
+00:46:18,590 --> 00:46:22,970
+منها vaccines و بنحكم فيها المرضى عشان نمنع حدوث
+
+640
+00:46:22,970 --> 00:46:27,650
+المرضى طبعا
+
+641
+00:46:27,650 --> 00:46:30,590
+هذا ليه دول مش special حفظ بس هذا بعطيكوا فكرة على
+
+642
+00:46:30,590 --> 00:46:35,710
+أن كل دولة في العالم عندها عندها قائمة قائمة
+
+643
+00:46:37,610 --> 00:46:45,330
+بالأمراض اللى بتطعم فيها او بتحمي أطفالها من خلال
+
+644
+00:46:45,330 --> 00:46:49,910
+ال vaccine منها فمثلا عندنا Hepatitis B Erotavirus
+
+645
+00:46:49,910 --> 00:46:53,150
+فى عندنا Diphtheria Tetanus Pertosis فى عندنا
+
+646
+00:46:53,150 --> 00:46:56,610
+Hemophilus Influenza Type B Hemophilus Influenza
+
+647
+00:46:56,610 --> 00:46:59,270
+لسه الحين حاكينا عنها احنا مظهور Hemophilus
+
+648
+00:46:59,270 --> 00:47:01,290
+Influenza Hemophilus Influenza Hemophilus
+
+649
+00:47:01,290 --> 00:47:01,450
+Influenza Hemophilus Influenza Hemophilus
+
+650
+00:47:01,450 --> 00:47:01,450
+Influenza Hemophilus Influenza Hemophilus
+
+651
+00:47:01,450 --> 00:47:01,490
+Influenza Hemophilus Influenza Hemophilus
+
+652
+00:47:01,490 --> 00:47:01,670
+Influenza Hemophilus Influenza Hemophilus
+
+653
+00:47:01,670 --> 00:47:03,250
+Influenza Hemophilus Influenza Hemophilus
+
+654
+00:47:03,250 --> 00:47:03,250
+Influenza Hemophilus Influenza Hemophilus
+
+655
+00:47:03,250 --> 00:47:03,650
+Influenza Hemophilus Influenza Hemophilus
+
+656
+00:47:03,650 --> 00:47:06,010
+Influenza Hemophilus Influenza Hemophفين ال polio
+
+657
+00:47:06,010 --> 00:47:09,950
+virus؟ و ال polio أنا قولتلكوا أنه فلسطين خالية من
+
+658
+00:47:09,950 --> 00:47:15,610
+ال polio من أكتر من 25 سنة تمام؟ ف .. جيد كتير إنه
+
+659
+00:47:15,610 --> 00:47:19,430
+نعرف هذا الكلام missiles, mumps, rubella في
+
+660
+00:47:19,430 --> 00:47:22,190
+الأددية أن أنتوا أخدتوا ماهو ال vaccine؟ في
+
+661
+00:47:22,190 --> 00:47:25,130
+الأددية أخدتوا vaccine؟ عارفين ماهو ال vaccine
+
+662
+00:47:25,130 --> 00:47:30,570
+اللي أخدته؟ لأ ال rubella ال rubella اللي هي
+
+663
+00:47:30,570 --> 00:47:35,130
+الحصبة الألمانيةهذه خطيرة بتعمل complications في
+
+664
+00:47:35,130 --> 00:47:38,230
+ال pregnancy احنا عندنا في البلدان العربية ان سن
+
+665
+00:47:38,230 --> 00:47:40,950
+الزواج بيكون مخافض قد يبدأ من الأددية، مظبوط ولا
+
+666
+00:47:40,950 --> 00:47:44,530
+لا؟ قد يبدأ من الأددية فعشان هيك حطوا السن ..
+
+667
+00:47:44,530 --> 00:47:47,510
+شوفوا احنا بتعملوا في المبادرة الحالية السن تبع ال
+
+668
+00:47:47,510 --> 00:47:51,530
+vaccination طبعا انتوا أخدتواه انتوا صغار، بس
+
+669
+00:47:51,530 --> 00:47:54,750
+عملناله جرعة داعمة وانتوا كبار، تمام؟ وانتوا كبار
+
+670
+00:47:54,750 --> 00:48:00,080
+عملناله جرعة داعمةالفيروس سن ال vaccination له
+
+671
+00:48:00,080 --> 00:48:03,300
+علاقة بعض الأحيان ببعض ال behaviors في البلدان
+
+672
+00:48:03,300 --> 00:48:10,100
+الغربية بيطعموا البنات الجناية من سن ال 11 ل 12 ضد
+
+673
+00:48:10,100 --> 00:48:11,900
+فيروس اسمه
+
+674
+00:48:14,220 --> 00:48:18,220
+Papilloma virus السن هذه بالذات هي بالنسبة لهم
+
+675
+00:48:18,220 --> 00:48:21,760
+السن الناشطة جنسية تمام؟ وبالتالي فيه هذا الفيروس
+
+676
+00:48:21,760 --> 00:48:25,320
+بسبب مرض بينتقل عن طريق الجنس و قد يسبب cancer
+
+677
+00:48:25,320 --> 00:48:30,140
+فانت سبب دي ايه؟ cancer فاختاروا السن هذا للتطييب
+
+678
+00:48:30,140 --> 00:48:35,240
+عشان هو السن اللي ايه الملائم للحماية من المرض
+
+679
+00:48:35,460 --> 00:48:39,400
+انتوا اختاروا سن الاددية عشان سن الزواج بقولنا انه
+
+680
+00:48:39,400 --> 00:48:45,620
+لو صار حامل والست ماعندهاش immunity جيدة لرنبالا
+
+681
+00:48:45,620 --> 00:48:49,880
+قد يحدث complication وقد يحدث اما congenital
+
+682
+00:48:49,880 --> 00:48:54,360
+abnormalities او حتى abortion طبعا تلاحظوا هنا
+
+683
+00:48:54,360 --> 00:48:58,370
+birthone month, two month, four month يبقى فينا
+
+684
+00:48:58,370 --> 00:49:01,650
+هنا يعني اللي هي الفترة الزمنية و .. و كام جرعة
+
+685
+00:49:01,650 --> 00:49:05,810
+بناخد مثلا ال hemophilus influenza type B هكام
+
+686
+00:49:05,810 --> 00:49:11,130
+جرعة؟ عشرين و أربع شهور و إيه؟ ست شهور و ممكن نعطي
+
+687
+00:49:11,130 --> 00:49:14,530
+كمان جرعة دايمة ما بين اتناشر و خمساشر شهر و ما
+
+688
+00:49:14,530 --> 00:49:18,650
+بين تسعتاشر اتناشر يعني في عندي ..اما specific
+
+689
+00:49:18,650 --> 00:49:22,770
+period او range of period في جرعات رئيسية و في
+
+690
+00:49:22,770 --> 00:49:27,110
+حالة بيسميها booster dose بوستر لجرعة الدائمة جرعة
+
+691
+00:49:27,110 --> 00:49:30,690
+الدائمة طبعا في كمان vaccination للكبار من 7 ل 10
+
+692
+00:49:30,690 --> 00:49:34,130
+و من 11 ل 12 و من 12 هاي اللي قلتلكوا قبل ان انا
+
+693
+00:49:34,130 --> 00:49:38,430
+جابه شوية اللي هو الهيمن papillomavirus عطوا range
+
+694
+00:49:38,430 --> 00:49:40,330
+من 11 ل 12 سنة
+
+695
+00:49:42,690 --> 00:49:45,990
+طبعا ال artificial passive immunization تقريبا نفس
+
+696
+00:49:45,990 --> 00:49:49,670
+الأشياء بس بدل ما نعطي vaccine نعطي immunotherapy
+
+697
+00:49:49,670 --> 00:49:53,490
+بالنسبة لل treatment احنا ندور واحد في ال
+
+698
+00:49:53,490 --> 00:49:58,190
+treatmentاللي هو اعطاه صورة للمريض .. للطبيب عفواً
+
+699
+00:49:58,190 --> 00:50:02,630
+عن ال microbe اللي عزلناها و عن مدى حساسيته او
+
+700
+00:50:02,630 --> 00:50:06,930
+مقاومته لقائمة و هذه القائمة من الموضوعات الحيوية
+
+701
+00:50:06,930 --> 00:50:12,050
+بدها علم عشان نختارها يعني مش قائمة أشوائية و هيدا
+
+702
+00:50:12,050 --> 00:50:14,850
+هدرسها ان شاء الله معظمها في ال .. ال .. ال
+
+703
+00:50:14,850 --> 00:50:17,130
+diagnostic اللي بيتكلم عن ال treatment لكل microbe
+
+704
+00:50:17,130 --> 00:50:20,530
+من ال microbeهكذا يعني بيكون هنا ان شاء الله
+
+705
+00:50:20,530 --> 00:50:26,210
+محاضرتنا المحاضرة القادمة هبدأ بال micro organs
+
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/MKQTJ5RDVMA.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/MKQTJ5RDVMA.srt
new file mode 100644
index 0000000000000000000000000000000000000000..a544cb0637ed2a5776dfb538eb498d56d75295a1
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/MKQTJ5RDVMA.srt
@@ -0,0 +1,2719 @@
+1
+00:00:21,510 --> 00:00:24,710
+بسم الله الرحمن الرحيم اليوم.. اليوم إن شاء الله
+
+2
+00:00:24,710 --> 00:00:27,970
+هنكمل الموضوع اللي اتكلمنا فيه اللي هو ال
+
+3
+00:00:27,970 --> 00:00:33,150
+antibiotics أو ال antimicrobial تفكير بس بالعناوين
+
+4
+00:00:33,150 --> 00:00:37,570
+كنا بنعرف أنه قسمنا ال antibacterial chemically
+
+5
+00:00:37,570 --> 00:00:41,030
+داخلنا فينا حاجة اسمها penicillins و macrolides و
+
+6
+00:00:41,030 --> 00:00:45,230
+erythromycin أفضل تفرغ سايكليز وبعدين قسمناهم
+
+7
+00:00:45,230 --> 00:00:48,490
+بكتيري سايبر بكتيريو aesthetic broad spectrum و
+
+8
+00:00:48,490 --> 00:00:52,330
+narrow spectrum وآخر تقسيمة كانت دي الأهم من على
+
+9
+00:00:52,330 --> 00:00:55,770
+الإطلاق اللي هي based on a side effected cell
+
+10
+00:00:55,770 --> 00:00:58,790
+walls, inhibitors, cell membrane, protein
+
+11
+00:00:58,790 --> 00:01:02,470
+synthesis, DNA, or lipid acid وآخر إيش كان عندي
+
+12
+00:01:02,470 --> 00:01:05,230
+اللي هو ال Anti-metabolites أعتقد أن هذه اللوحة
+
+13
+00:01:05,230 --> 00:01:10,370
+توقفنا عنها المرة الماضية إيش يعني Anti
+
+14
+00:01:10,370 --> 00:01:15,010
+-metabolites؟ لو طلبنا عندي توأم، توأم متشابهين،
+
+15
+00:01:15,010 --> 00:01:20,620
+هل أحكيناها؟ توأم متشابهين واحد أنا بعطيه مبلغ من
+
+16
+00:01:20,620 --> 00:01:23,880
+المال كل يوم اللي بشوفه بعطيه مبلغ من المال، هذا
+
+17
+00:01:23,880 --> 00:01:27,380
+الشخص بيشتري كل الأدوات اللي لازم من المنزل ولا
+
+18
+00:01:27,380 --> 00:01:32,640
+أخوته ولا أخواته، والحياة بتستمر، التاني شق
+
+19
+00:01:32,640 --> 00:01:36,400
+التاني بالتوأم، والذي لا أستطيع تمييزه عن الأول لو
+
+20
+00:01:36,400 --> 00:01:41,080
+ما بقيده على حد الفلوس، ببطر على جزمه، بس هذا
+
+21
+00:01:41,080 --> 00:01:45,220
+الشخص التاني بياخد الفلوس وبحطهم في جيبه مش هيشتري
+
+22
+00:01:45,220 --> 00:01:48,500
+أي حاجة من خلية ولا.. يعني الحالة إيش هتصرف..
+
+23
+00:01:48,500 --> 00:01:52,900
+هتصرف.. هتجف أنا اللي ما بنش يميز بين اتنينهم
+
+24
+00:01:52,900 --> 00:01:58,120
+بيشبهوا بعض خدوا بالكم مش identical لكن بيشبهوا
+
+25
+00:01:58,120 --> 00:02:01,740
+بعض ولا سطر الإنزايم أن يميز بين ال substrate
+
+26
+00:02:01,740 --> 00:02:05,100
+الحقيقي وبين ال analog تبع ال analog اللي هو
+
+27
+00:02:05,100 --> 00:02:13,450
+الشريط أو النظير هذه الفكرة موجودة في مركبين
+
+28
+00:02:13,450 --> 00:02:16,090
+مشهورين جدا في ال Antimicrobials اسمهم
+
+29
+00:02:16,090 --> 00:02:21,470
+Sulfamethexazol والتاني اسمه Trimethoprim كلا هما
+
+30
+00:02:21,470 --> 00:02:24,850
+بيقولوا بقى بالمهمة طلعوا على الصفحات التانية
+
+31
+00:02:24,850 --> 00:02:28,410
+الاندرجرام
+
+32
+00:02:28,410 --> 00:02:34,680
+اللي أمامكم هو بيوضح pathway مسار.. مسار لتخليق
+
+33
+00:02:34,680 --> 00:02:37,900
+folic acid خدوا بعرفوا ال folic acid مهم جدا
+
+34
+00:02:37,900 --> 00:02:42,180
+لتصنيع مين؟ لتصنيع ال nucleotides وال nucleotides
+
+35
+00:02:42,180 --> 00:02:47,540
+مهمة لتصنيع ال DNA وال RNA بدون.. بدون.. بدون
+
+36
+00:02:47,540 --> 00:02:50,760
+folic acid أقصد لا مصدر أنه وصلنا ل nucleotide ولا
+
+37
+00:02:50,760 --> 00:02:57,880
+حصلنا DNA و RNA طيب كيف بنوجه تصنيع ال folic acid؟
+
+38
+00:02:57,880 --> 00:03:02,960
+شوفوا أول مركب اسمه بابا اللي هو Para Amino Benzoic
+
+39
+00:03:02,960 --> 00:03:05,440
+Acid البارا أمينو بنزويك أسيد البارا أمينو بنزويك
+
+40
+00:03:05,440 --> 00:03:06,100
+أسيد البارا أمينو بنزويك أسيد البارا أمينو بنزويك
+
+41
+00:03:06,100 --> 00:03:06,460
+أسيد البارا أمينو بنزويك أسيد البارا أمينو بنزويك
+
+42
+00:03:06,460 --> 00:03:08,780
+أسيد البارا أمينو بنزويك أسيد البارا أمينو بنزويك
+
+43
+00:03:08,780 --> 00:03:13,160
+أسيد البارا
+
+44
+00:03:13,160 --> 00:03:16,980
+أمينو بنزويك أسيد البارا أمينو بنزويك أسيد البارا
+
+45
+00:03:16,980 --> 00:03:21,660
+أمينو بنزويك أسيد البارا
+
+46
+00:03:21,660 --> 00:03:26,980
+أمينو بنزويك أسيد البارا أمينو بنزويك أسيد البارا
+
+47
+00:03:26,980 --> 00:03:32,420
+أمينو بنزويك أسيد البارا أمينو بنزويك أسيد المركب
+
+48
+00:03:32,420 --> 00:03:37,780
+analog لـdihydrofolic acid اللي المفروض يتحول
+
+49
+00:03:37,780 --> 00:03:42,280
+لـtetrahydrofolic acid يبقى لو كان فيه
+
+50
+00:03:42,280 --> 00:03:45,900
+dihydrofolic acid مش هيتحول لـtetrahydrofolic acid
+
+51
+00:03:45,900 --> 00:03:49,700
+مش هيتصنع الـfolic acid يبقى سلسلة مركبين
+
+52
+00:03:49,700 --> 00:03:53,700
+كـanalogues كـanalogues هدول المركبين اشتغلوا على
+
+53
+00:03:53,700 --> 00:03:57,280
+خطوطين مختلفتين يعني أنت بتتخيلوا مع بعض أن هو
+
+54
+00:03:57,280 --> 00:04:01,390
+يبقى في ويبقى في ويبقى في شغلين اللي بيفلت من الأول
+
+55
+00:04:01,390 --> 00:04:05,970
+ما فلتش من التاني فعطينا التانين مع بعض وعملناهم
+
+56
+00:04:05,970 --> 00:04:09,950
+مركب واحد بيبنيهم في السيداليات باسم واحد cell
+
+57
+00:04:09,950 --> 00:04:12,770
+from the hexasol تراكشحتا تراكشحتا تراكشحتا
+
+58
+00:04:12,770 --> 00:04:13,810
+تراكشحتا تراكشحتا تراكشحتا تراكشحتا تراكشحتا
+
+59
+00:04:13,810 --> 00:04:13,890
+تراكشحتا تراكشحتا تراكشحتا تراكشحتا تراكشحتا
+
+60
+00:04:13,890 --> 00:04:14,490
+تراكشحتا تراكشحتا تراكشحتا تراكشحتا تراكشحتا
+
+61
+00:04:14,490 --> 00:04:21,390
+تراكشحتا تراكشحتا تراكشحتا
+
+62
+00:04:21,390 --> 00:04:29,390
+تراكشحت
+
+63
+00:04:30,020 --> 00:04:34,840
+أما عن طريق ارتباط الأنالوج بالـ Active site بتاع
+
+64
+00:04:34,840 --> 00:04:36,840
+الإنزايم اللي هو Active site واللي هو الـ
+
+65
+00:04:36,840 --> 00:04:40,620
+Allosteric site أو عن طريق الارتباط بالـ
+
+66
+00:04:40,620 --> 00:04:44,980
+Allosteric site لتغيير الـ Active site احنا اللي
+
+67
+00:04:44,980 --> 00:04:49,740
+بنتكلم عنه الآن هو عبارة عن ارتباط لشيء شبيه بالـ
+
+68
+00:04:49,740 --> 00:04:51,340
+Substrate على الـ Active
+
+69
+00:04:54,360 --> 00:04:57,940
+بيسموه competitive inhibitors، بيسموه structural
+
+70
+00:04:57,940 --> 00:05:02,860
+analog، بيسموه antimicrobials، أمام؟ فهذه
+
+71
+00:05:02,860 --> 00:05:06,740
+الميكانيزم الخامسة اللي احنا قلنا عنها، بيقسم
+
+72
+00:05:06,740 --> 00:05:11,360
+البكتيريا إلى خمسة، اليوم هنتقل الصحيح إلى موضوع
+
+73
+00:05:11,360 --> 00:05:14,940
+ال testing لل antibiotics، وهذا أكثر موضوع مهم وده
+
+74
+00:05:14,940 --> 00:05:18,680
+أركز فيه لإنه في أخطاء قاتلة في مختبراتنا في هذا
+
+75
+00:05:18,680 --> 00:05:25,610
+البرنامج إذا أردنا أن نقوم بدراسة.. بدراسة مدى
+
+76
+00:05:25,610 --> 00:05:30,610
+فعالية مضاد حيوي معين على بكتيريا معينة.. بصيرش
+
+77
+00:05:30,610 --> 00:05:34,550
+أهالي اتنين وأعملهم مسارعة وواحد قرر بتقدر يربط
+
+78
+00:05:34,550 --> 00:05:38,830
+رجليه ويقول فلان غلق فلان.. بكدا بيكون في نوع من
+
+79
+00:05:38,830 --> 00:05:44,170
+ال biasness يعني مغوظين مع حدا.. لازم أ offer
+
+80
+00:05:44,170 --> 00:05:48,150
+للتانين نفس ال chances.. نفس الفرصة فبالتالي أنا
+
+81
+00:05:48,150 --> 00:05:54,050
+أقوم بهذه المهمة عن طريق توفير وسط ملائم جدا لنمو
+
+82
+00:05:54,050 --> 00:05:58,730
+البكتيريا بنزرع البكتيريا بشكل طبقة ونحط أقراص من
+
+83
+00:05:58,730 --> 00:06:02,330
+ال antibiotics تحتوي على تركيز محدد زي ما تشوفين
+
+84
+00:06:02,330 --> 00:06:08,010
+هنا كل قرص من الأقراص اللي موجودة إيه الرمز وتركيز
+
+85
+00:06:08,510 --> 00:06:11,830
+الرمز والتركيز والرمز يدل على نوع ال antibiotics
+
+86
+00:06:11,830 --> 00:06:15,450
+طبعا الرموز تختلف من شركة لشركة وبالتالي لما نمارس
+
+87
+00:06:15,450 --> 00:06:19,730
+المهنة بتاعتنا بيكون عندنا جدول ال antibiotics
+
+88
+00:06:19,730 --> 00:06:23,910
+اللي استخدمناهم من شركة معينة وهي بتعطينا اصلا ال
+
+89
+00:06:23,910 --> 00:06:26,390
+abbreviations اللي هي الاختصارات ليهاش كل واحد
+
+90
+00:06:26,390 --> 00:06:31,110
+اختصار وليهاش كل واحد أو كل concentration لكل
+
+91
+00:06:31,110 --> 00:06:36,900
+course موجودة وضعنا الآن تقريبا على السبورة تقريبا
+
+92
+00:06:36,900 --> 00:06:40,300
+على الطبق اللي حطينا فيه البكتيريا بشكل طبقة
+
+93
+00:06:40,300 --> 00:06:46,980
+وحطينا عليه أقراص من ال antimicrobials اتنين
+
+94
+00:06:46,980 --> 00:06:50,520
+حطيناهم في ال incubator وعطيناهم فرصة عطينا فرصة
+
+95
+00:06:50,520 --> 00:06:53,760
+لل antibiotic أن يعمل diffusion وعطينا فرصة
+
+96
+00:06:53,760 --> 00:06:56,980
+للبكتيريا تنمو لانونامي على وسط أنا هزلها على وسط
+
+97
+00:06:56,980 --> 00:07:03,560
+لأنهم بروا الصورة لأن أنا حاولت القوس في منطقة خالية
+
+98
+00:07:03,560 --> 00:07:07,040
+من النمو، خالية من النمو، ما بعرفش killing أو
+
+99
+00:07:07,040 --> 00:07:10,940
+inhibition، لكن هي خالية من النمو، بيسميها zone of
+
+100
+00:07:10,940 --> 00:07:15,360
+inhibition، مهم جدا المصطلح zone of inhibition لأن
+
+101
+00:07:15,360 --> 00:07:17,480
+هو اللي بينجيسه، هو اللي بينجيسه
+
+102
+00:07:35,070 --> 00:07:39,310
+كلمة أحسن مش موجودة في قاموسنا على الإطلاق صارت في
+
+103
+00:07:39,310 --> 00:07:40,690
+الميكروبيولوجي
+
+104
+00:07:44,500 --> 00:07:48,960
+الدرج أن ال technician بتطلع على الطبق وبيقول هذا
+
+105
+00:07:48,960 --> 00:07:51,580
+زورة كبيرة، هذا sensitive، هذا زورة موسطة، هذا
+
+106
+00:07:51,580 --> 00:07:54,740
+intermediary، هذا زورة صغيرة، هذا resistant، هذا
+
+107
+00:07:54,740 --> 00:07:59,740
+الكلام أكبر جريمة ممكن يرتكبها واحد بيقدم خدمة
+
+108
+00:07:59,740 --> 00:08:04,320
+microbiology ودلوقت موجودة أن في القطاع ومنتشرة
+
+109
+00:08:04,320 --> 00:08:10,700
+بينهم ويشجع حلقات أطباق بعد مواضعتهم لل protocol
+
+110
+00:08:10,700 --> 00:08:17,270
+المستخدمة في المختبرات طيب إيش الصح؟ قبل الصح بنسأل
+
+111
+00:08:17,270 --> 00:08:22,850
+السؤال، في هدا فيكم بيقدر يميز بين 16 millimeter و
+
+112
+00:08:22,850 --> 00:08:26,270
+17 millimeter بالعين المجردة بتاعتك؟ يعني تطلع و
+
+113
+00:08:26,270 --> 00:08:31,400
+هيك 0.16 أو يجيسها هو.. أقول لك 16 و17 ممكن نقول من
+
+114
+00:08:31,400 --> 00:08:34,700
+أكبر من التانية الفرق بسيط جدا لدرجة أنه ما بنقدرش
+
+115
+00:08:34,700 --> 00:08:37,580
+.. احنا بنشوفهم متساويين.. بنشوفهم متساويين في
+
+116
+00:08:37,580 --> 00:08:41,000
+غالب الأمر هذا أول سؤال سألته أنا.. ما بنقدرش احنا
+
+117
+00:08:41,000 --> 00:08:43,420
+بالعين المجردة بنميز بين أرقام قليلة من بعض
+
+118
+00:08:43,420 --> 00:08:47,120
+فالأصل في الأشياء القياس.. إنه نجيب مصطرة أو
+
+119
+00:08:47,120 --> 00:08:49,640
+caliber.. تعرفون ال caliber في هيئة الفيزياء؟ اللي
+
+120
+00:08:49,640 --> 00:08:55,000
+بنجيس فيه الأقطار.. بنحطه وبنجيس قطر وبنسجل..
+
+121
+00:08:55,000 --> 00:08:57,580
+يبقى الزواق وال generation مش بالأعلى
+
+122
+00:09:00,390 --> 00:09:04,290
+أول نقطة، نقطة تانية تفسير الأرقام اللي طلعت
+
+123
+00:09:04,290 --> 00:09:08,400
+معانا، إيه تفسير الأرقام؟ طول معايا 17، إيش يعني
+
+124
+00:09:08,400 --> 00:09:11,600
+17؟ طول معايا 20، إيش يعني 20؟ هل كل ال
+
+125
+00:09:11,600 --> 00:09:15,000
+antibiotics أرقامهم واحد بتفسيراتهم؟ لأ، أنا بقول
+
+126
+00:09:15,000 --> 00:09:18,260
+فيها lista قائمة بأسماء الـ antibiotics بالـ
+
+127
+00:09:18,260 --> 00:09:22,400
+abbreviation وفي ثلاث أعمدة كمان، إيش الأعمدة دي؟
+
+128
+00:09:22,400 --> 00:09:28,260
+RIS، R يعني resistant، I intermediate، S يعني 
+
+129
+00:09:28,260 --> 00:09:32,190
+sensitive أو susceptible، نفس المعنى Susceptible
+
+130
+00:09:32,190 --> 00:09:35,010
+لأنه حتى بيقول Antibiotic Sensitivity أو
+
+131
+00:09:35,010 --> 00:09:40,530
+Antibiotic Susceptibility نفس المعنى كم عمود صار؟
+
+132
+00:09:40,530 --> 00:09:44,710
+طب Antibiotic Abbreviation Disk Potency أو
+
+133
+00:09:44,710 --> 00:09:50,570
+Concentration RIS إيش يعني R بيبقى resistant؟ بيكون
+
+134
+00:09:50,570 --> 00:09:59,490
+فيه رقم إذا أقل من فهي R إذا بين X و Y فهي I إذا 
+
+135
+00:09:59,490 --> 00:10:04,590
+كان أكثر من أو يساوي فهي S يبقى في الآخر آية بدي
+
+136
+00:10:04,590 --> 00:10:10,590
+أجيب والرقم اللي بيطلع أطبقه على مين؟ على ترجمة و
+
+137
+00:10:10,590 --> 00:10:16,430
+أشوف هل هو R ولا I ولا S طب أعطيكم أمثلة مش دقيقة
+
+138
+00:10:16,430 --> 00:10:23,180
+لكن بتعبّر على الفكرة تبقى تعرف البنسلين؟ وفي حدث
+
+139
+00:10:23,180 --> 00:10:25,860
+ما جنتاميسين جنتاميسين مرة بعد محاضرة السابقة
+
+140
+00:10:25,860 --> 00:10:28,700
+في الـ amino glycoside والبنسبريب هو الـ superstar
+
+141
+00:10:28,700 --> 00:10:35,780
+أولًا أنت بدي أصلاً أن أعرف Staphylococcus aureus إذا
+
+142
+00:10:35,780 --> 00:10:43,120
+كان الرقم 25 millimeter في البنسلين بيكون
+
+143
+00:10:43,120 --> 00:10:48,810
+resistant بالإضافة لو كان رقم 17 في الـ gentamicin
+
+144
+00:10:48,810 --> 00:10:53,870
+بتكون sensitive، مين أكبر؟ الـ 25 و 17 وأعتقد أن
+
+145
+00:10:53,870 --> 00:10:59,810
+علامة بتميز بين 25 و 17 وهذا أكبر من الثاني ومع
+
+146
+00:10:59,810 --> 00:11:03,810
+ذلك مين الـ sensitive كانت؟ الـ 17 كانت sensitive والـ
+
+147
+00:11:03,810 --> 00:11:07,750
+25 كانت resistant، إذا الـ zone مرتبطة بنوع الـ
+
+148
+00:11:07,750 --> 00:11:13,090
+antibiotic وقد تكون مرتبطة بنوع البكتيريا أيضًا إذا
+
+149
+00:11:13,090 --> 00:11:17,390
+بنفهم من القيامة إنه لا قيادة.. لا.. لا فتوة لا
+
+150
+00:11:17,390 --> 00:11:23,090
+إصدار نتائج based on visual inspection ولا جداول
+
+151
+00:11:23,090 --> 00:11:29,070
+مبنية على اللي هو القطر، دولة رجوها إلى الجداول اسم
+
+152
+00:11:29,070 --> 00:11:33,110
+الطريقة هذه disk diffusion method ليش سموها disk
+
+153
+00:11:33,110 --> 00:11:36,450
+diffusion method؟ لأنه بنحط من أقراص ولأن لو نحط
+
+154
+00:11:36,450 --> 00:11:41,390
+الأقراص إحنا الـ diffusion تبعها بشكل باطل، ده أيضًا
+
+155
+00:11:41,390 --> 00:11:48,040
+disk diffusionبدأنا اختراع قراهيب نطلع عليه اسمه
+
+156
+00:11:48,040 --> 00:11:51,680
+E-test ممكن نصبعه على شوية وبعدين نرجعله نصبعه
+
+157
+00:11:51,680 --> 00:11:55,480
+على شوية وبعدين نرجعله نشوف حاجة ثانية اسمها MIC
+
+158
+00:11:55,480 --> 00:12:00,520
+و MBC الـ MBC يعني مش القناة الـ MBC وإنما هي اللي
+
+159
+00:12:00,520 --> 00:12:06,660
+هي Minimum Bacterial Cycle Concentration برضه
+
+160
+00:12:06,660 --> 00:12:10,080
+ركزوا معايا الأهالي تركيز جيد عشان نفهم إيش يعني
+
+161
+00:12:10,080 --> 00:12:15,000
+MIC وإيش يعني MBC فكيف نقول أن الـ antibiotic ممكن
+
+162
+00:12:15,000 --> 00:12:17,900
+يكون بكتيري سايدل أو ممكن يكون بكتيري سايدل؟ وإذا
+
+163
+00:12:17,900 --> 00:12:22,080
+بتتذكر المحاضرة ارجعوا لها هتلاقوها، لقول جملة إن
+
+164
+00:12:22,080 --> 00:12:25,240
+static أو سايدل is a matter of concentration إذا
+
+165
+00:12:25,240 --> 00:12:29,140
+اختلفت الـ concentration هو سايدل لكن قررنا إنه
+
+166
+00:12:29,140 --> 00:12:31,400
+سايدل الـ antibiotic أو الـ static based on the
+
+167
+00:12:31,400 --> 00:12:36,060
+therapeutic dose على الجرعة العلاجية هل هو static
+
+168
+00:12:36,060 --> 00:12:41,020
+ولا سايدل؟ هل من الممكن أننا في المختبر نحدد الـ
+
+169
+00:12:41,020 --> 00:12:44,800
+MIC والـ MBC نعدي لحدها بكل سهولة ويسر بالطريقة
+
+170
+00:12:44,800 --> 00:12:48,100
+اللي قدامكم اللي بتقول أن في عندي مجموعة من
+
+171
+00:12:48,100 --> 00:12:51,860
+القنابيب شايفينها؟ مجموعة من القنابيب من واحد
+
+172
+00:12:51,860 --> 00:12:55,100
+لحد تسعة والأخيرة control control يعني بنحط
+
+173
+00:12:55,100 --> 00:12:59,080
+فيها بكتيريا عشان نضمن أن.. ما يبحثش انتباهك عشان
+
+174
+00:12:59,080 --> 00:13:03,280
+نضمن أن البكتيريا تمر في عاملة turbidityالقنابيب
+
+175
+00:13:03,280 --> 00:13:08,160
+حطينا من واحد على تركيز نصه في اثنين ونصه في
+
+176
+00:13:08,160 --> 00:13:12,080
+ثلاثة، نصه يعني عملنا CGAPUSA يعني لو كان
+
+177
+00:13:12,080 --> 00:13:16,980
+واحد جرعة نص جرعة ربع جرعة، واحد كده لما نص لآخر
+
+178
+00:13:16,980 --> 00:13:21,080
+الآخر، بعدين حطينا على القنابيب كلياتهم نفس الـ
+
+179
+00:13:21,080 --> 00:13:25,160
+equal volume من البكتيريا تمام؟ وحطيناهم في الـ
+
+180
+00:13:25,160 --> 00:13:29,020
+incubator وقولنا للبكتيريا وللـ antibiotic مين
+
+181
+00:13:29,020 --> 00:13:33,860
+يغلب بعد 24 ساعة طلعناهم من الـ incubator اللي جينا
+
+182
+00:13:33,860 --> 00:13:39,040
+رقم واحد مش فيها نمو طلع الأخيرة الـ control فيها
+
+183
+00:13:39,040 --> 00:13:41,660
+نمو وواضح أنها فيها نمو بينما الأولى مافيهاش نمو و
+
+184
+00:13:41,660 --> 00:13:44,820
+الثانية والثالثة والرابعة والخامسة بينما بدأ
+
+185
+00:13:44,820 --> 00:13:50,700
+النمو يظهر في السادسة طيب تعريف الـ MIC بيقول the
+
+186
+00:13:50,700 --> 00:13:53,900
+lowest concentration of antibiotics that inhibited
+
+187
+00:13:53,900 --> 00:13:56,060
+the growth of microorganisms
+
+188
+00:13:58,310 --> 00:14:01,910
+وين الـ lowest concentration اللي عملت inhibition
+
+189
+00:14:01,910 --> 00:14:07,110
+للـ growth of microorganism في القنابيب؟ الخامسة، السادسة
+
+190
+00:14:07,110 --> 00:14:12,370
+فيها نمو، هي اللي جابتها هي الـ lowest
+
+191
+00:14:12,370 --> 00:14:17,450
+concentration اللي عملت، مضبوط أو لا؟ أقل التركيز،
+
+192
+00:14:17,450 --> 00:14:19,910
+أقل الـ inhibition اللي هو الأنبوبة الخامسة، طب إذا أنا
+
+193
+00:14:19,910 --> 00:14:21,390
+عارف التركيز اللي هو الأنبوبة الأولى، كده عارف
+
+194
+00:14:21,390 --> 00:14:24,010
+التركيز اللي هو الأنبوبة الخامسة طبعًا عاملة serial
+
+195
+00:14:24,010 --> 00:14:28,510
+dilution، يبقى اخترنا الـ MIC بناء على the lowest
+
+196
+00:14:28,510 --> 00:14:30,430
+concentration that inhibited the growth of
+
+197
+00:14:30,430 --> 00:14:33,170
+microorganisms أو test organisms، مش الأقل الـ
+
+198
+00:14:33,170 --> 00:14:38,930
+MBC؟ إحنا الآن اللي صار مش عارفين هل هو قتل أصلاً
+
+199
+00:14:38,930 --> 00:14:43,290
+ولا.. ولا anything فبنعمل sub culture بس خدوا
+
+200
+00:14:43,290 --> 00:14:45,790
+بالكم الصورة اللي عندي هنا مجلوبة شوية، بلال ما هي
+
+201
+00:14:45,790 --> 00:14:51,330
+بدأ من واحد من هنا، لو واحد بدأ من هنا فمثلاً عندي
+
+202
+00:14:51,330 --> 00:14:56,030
+تركيز هنا ثمانية وهنا أربعة وهنا اثنين وهنا واحد
+
+203
+00:14:56,030 --> 00:15:00,660
+milligram الأخيرة مضروبة milligram شايفينها؟ في الخامس
+
+204
+00:15:00,660 --> 00:15:04,560
+قنابيب الأخيرة اللي هي نص جرام نص جرام عاملة
+
+205
+00:15:04,560 --> 00:15:10,660
+تربيتي عاملة تربيتي بجميع الـ MIC اللي جابتها صح؟
+
+206
+00:15:10,660 --> 00:15:14,060
+مش كانت آخر واحدة عاملة تربيتي اللي جابتها هي الـ
+
+207
+00:15:14,060 --> 00:15:18,160
+MIC روحنا أخذنا من كل القنابيب اللي صار فيهم
+
+208
+00:15:18,160 --> 00:15:21,800
+inhibition loop وزرعناها على دلوقت أجار أو على
+
+209
+00:15:21,800 --> 00:15:24,600
+رترد أجار أو على ملارتهم حسب الوضع أو حسب
+
+210
+00:15:24,600 --> 00:15:29,320
+البكتيريا لاحظوا اللي هو النمو صار هنا
+
+211
+00:15:39,560 --> 00:15:44,620
+يبقى مين أكثر بيكون في القيمة في الرقم الـ MBC ولا
+
+212
+00:15:44,620 --> 00:15:50,360
+الـ MIC كتركيز الـ
+
+213
+00:15:50,360 --> 00:15:54,980
+MBC بيكون تركيزه أعلى من الـ MIC شوفوا دلوقتي قاعدة
+
+214
+00:15:54,980 --> 00:16:01,680
+مستحيل أن الـ MBC تكون أقل من الـ MIC أقل عشان هذا
+
+215
+00:16:01,680 --> 00:16:04,940
+الكلام أقل concentration أقل concentration كلام
+
+216
+00:16:04,940 --> 00:16:07,940
+يعني مفهوم ولا مش مفهوم لسه؟ أنهيض.. أنهيض..
+
+217
+00:16:07,940 --> 00:16:15,350
+أنهيضه كمان مرة الآن الـ inhibition المفروض بيكون
+
+218
+00:16:15,350 --> 00:16:20,830
+تركيز لو زدت وأنا ممكن أصير killing الـ killing لو
+
+219
+00:16:20,830 --> 00:16:23,850
+مجرد ممكن أصير inhibition يبقى بالكم أن الـ
+
+220
+00:16:23,850 --> 00:16:28,930
+inhibition أقل من الـ killing أقل في التركيز أقل في
+
+221
+00:16:28,930 --> 00:16:32,670
+التركيز فعشان أنا أحصل على القتل أحصل على القتل
+
+222
+00:16:32,670 --> 00:16:37,470
+بدي اللي هو تركيز إيه؟ أعلى فبيعمل الـ MBC يا بيساوي
+
+223
+00:16:37,470 --> 00:16:42,410
+الـ MIC يا أعلى منها هيكون الصورة واضحة طبعًا بقيت
+
+224
+00:16:42,410 --> 00:16:47,890
+أنا عملتها كده وقفزت عن صورة بدرجة أعلى اللي هي E
+
+225
+00:16:47,890 --> 00:16:55,650
+-test الـ E-test اختراع عطولة strip الـ
+
+226
+00:16:55,650 --> 00:16:59,690
+concentration فيه gradient إيش يعني gradient من
+
+227
+00:16:59,690 --> 00:17:03,130
+أعلى إلى أسفل طبعًا تقنية خدوا باركم سهلة عملها
+
+228
+00:17:03,130 --> 00:17:07,090
+strip بلاستيك فبالتالي تقنية يبدو أنها patent يعني
+
+229
+00:17:07,090 --> 00:17:11,820
+محصولة براد اختراع فـ expensive يعني م.. م..
+
+230
+00:17:11,820 --> 00:17:15,040
+موفودش لازم تقنية إلا مرة واحدة لدراسة، عملنا
+
+231
+00:17:15,040 --> 00:17:20,660
+دراسة فيها، تمام؟ فمش common لكن الفكرة فيها إنه
+
+232
+00:17:20,660 --> 00:17:25,760
+تركيز مختلفة من Antibiotic 1 نحط على طبق خمسة،
+
+233
+00:17:25,760 --> 00:17:29,760
+خمس أنواع من الـ antibiotic انفحصوا في طبق واحد، بس
+
+234
+00:17:29,760 --> 00:17:32,760
+هذا أربع، واحد antibiotic
+
+235
+00:17:37,520 --> 00:17:43,000
+يبقى العملية هنا مضنية ومتعبة وبتاخد وقت وبتاخد
+
+236
+00:17:43,000 --> 00:17:48,560
+space فمن المقارنة مين أحسن؟ الـ E test طب الـ E
+
+237
+00:17:48,560 --> 00:17:51,460
+test مين أحسن؟ كان مرة اللي وجهنا نفس الغلطة الـ E
+
+238
+00:17:51,460 --> 00:17:58,400
+test جيد لكن expensive هذه رخيصة لكن متعبة يبقى كل
+
+239
+00:17:58,400 --> 00:18:01,860
+واحدة اللي كانت إيجابية أو سلبية تعالوا ناخد حل
+
+240
+00:18:01,860 --> 00:18:02,220
+وسط
+
+241
+00:18:06,620 --> 00:18:12,000
+الصورة اللي قدامنا أنصر
+
+242
+00:18:12,000 --> 00:18:15,740
+الجزر اللي مكتوب كتابها نطلع على الـ micro title
+
+243
+00:18:15,740 --> 00:18:18,740
+plate الـ micro title plate هو عبارة عن plate
+
+244
+00:18:18,740 --> 00:18:24,980
+بيحتوي على 96 wells 96 ثمانية في 12 عارفين ثمانية
+
+245
+00:18:24,980 --> 00:18:30,500
+اللي هم العمودي و 12 تعالى نبدأ من الجهة اليمنى في
+
+246
+00:18:30,500 --> 00:18:37,980
+الأطباق ناخذ هذه تركيز الـ antibiotic هنا نفترض أنه
+
+247
+00:18:37,980 --> 00:18:42,980
+واحد نفترض أنه واحد خففنا إيش سنة بعدين بيكون؟
+
+248
+00:18:42,980 --> 00:18:49,060
+نص، 125، 112 وربع.. إلى آخره اللي عند وين؟ اللي
+
+249
+00:18:49,060 --> 00:18:53,580
+عند آخر وين؟ antibiotic اثنين، هذا antibiotic واحد،
+
+250
+00:18:53,580 --> 00:18:56,140
+هذا اثنين، هاي ثلاثة، هاي أربعة، خمسة، ستة، سبعة
+
+251
+00:18:56,140 --> 00:18:59,420
+الأولاني عملناها كـ control كما تلاحظوا هنا فيها
+
+252
+00:18:59,420 --> 00:19:06,120
+بوستر graph control فممكن أفحص فيه أنا أو أبحث 
+
+253
+00:19:06,120 --> 00:19:10,700
+ثمانية وممكن أبحث أتناشر إذا أنا كنت عندي معرفة 
+
+254
+00:19:10,700 --> 00:19:15,980
+مسبقة بالتركيز المتوقع قبل ما أبدأ التركيز من
+
+255
+00:19:15,980 --> 00:19:21,680
+العدد الأتناشر فانزل من واحد ونزل وانزل كعمدة عمل
+
+256
+00:19:21,680 --> 00:19:25,720
+تخفيف بشكل عمودي ولا بشكل أفقي فهيك بدي تعرف 
+
+257
+00:19:25,720 --> 00:19:29,260
+الأنواع من الـ antibiotic بس بقلل مين التخفيفة بقلل
+
+258
+00:19:29,260 --> 00:19:37,280
+التخفيفة عمل تخفيف للـ antibiotic X وY وZ اللي هم 
+
+259
+00:19:37,280 --> 00:19:42,100
+بالعدد السابع هدول حطيت عليهم نفس البكتيريا نفس
+
+260
+00:19:42,100 --> 00:19:44,720
+البكتيريا Staphylococcus aureus على سبيل المثال
+
+261
+00:19:44,720 --> 00:19:50,700
+الآن كلهم بيحتووا على نفس العدد من البكتيريا الشيء
+
+262
+00:19:50,700 --> 00:19:55,060
+المختلف هو الـ antibiotic وتركيزه الـ antibiotic و
+
+263
+00:19:55,060 --> 00:19:58,020
+تراكيزه أخدت الـ microheater plate ودده على الـ
+
+264
+00:19:58,020 --> 00:20:02,540
+incubator 24 ساعة طلعت مشكلتي فيها اللي بنعمل
+
+265
+00:20:02,540 --> 00:20:06,830
+فحصات أن الـ turbidity مش واضحة يعني أنا ممكن أقول
+
+266
+00:20:06,830 --> 00:20:11,890
+turbidity مش turban والاختلاف مع بعض على أن مين الـ
+
+267
+00:20:11,890 --> 00:20:14,690
+turbidity وكلمة turbidity في الحلقة بتعرفين هي بتحدد من
+
+268
+00:20:14,690 --> 00:20:19,990
+أن في نمو ومش هي نمو ففي اختلاف عزيز بنضيفه على
+
+269
+00:20:19,990 --> 00:20:24,290
+الـ media اللي هو تترازوليوم كلوريد تترازوليوم
+
+270
+00:20:24,290 --> 00:20:32,150
+كلوريد من ورا عرفته عادة في الـ motility test نفس
+
+271
+00:20:32,150 --> 00:20:36,400
+المركبات اللي سميناها في الـ motility test طيب وضعناها
+
+272
+00:20:36,400 --> 00:20:41,880
+بعد الـ incubation بكميات محددة على كل الأطفال إذا
+
+273
+00:20:41,880 --> 00:20:45,620
+في بكتيريا بناك بصير اللون أحمر إذا ما فيش بكتيريا
+
+274
+00:20:45,620 --> 00:20:49,480
+بيظل اللون كذا لأ طبعا الـ ... الـ ... الـ ... الـ ...
+
+275
+00:20:49,480 --> 00:20:53,600
+المادة المضافة كانت زرقاء أو تبروها فاضية ما فيش
+
+276
+00:20:53,600 --> 00:20:59,170
+لون وين بيتحول اللون؟ بعدها دي صح اللي جالك تتقولها
+
+277
+00:20:59,170 --> 00:21:02,090
+إيه MIC إيه الـ MIC؟ إيه الـ MIC؟ إيه الـ MIC؟ وأنا 
+
+278
+00:21:02,090 --> 00:21:07,070
+عامل X هي طلع الـ MIC وين؟ هنا، لهنا، من ضمنك، و
+
+279
+00:21:07,070 --> 00:21:12,410
+أول وأحسن بأول وأحسن حكي إنه طيب السؤال كلمة
+
+280
+00:21:12,410 --> 00:21:16,890
+مين أحسن؟ مين أحسن؟ لأن المرة قعدت مين أحسن قعدت
+
+281
+00:21:16,890 --> 00:21:22,770
+مين أحسن مش فيها خبعة أنت بيتك الـ MIC دبعته كبيرة
+
+282
+00:21:22,770 --> 00:21:28,560
+في الرقم ولا صغيرة؟ كل ما ذكر الـ MIC تبقى يعني إيه؟
+
+283
+00:21:28,560 --> 00:21:32,340
+الـ effectiveness صحيح؟ الـ effectiveness فكل اللي
+
+284
+00:21:32,340 --> 00:21:35,800
+بيصوره يعني الملعقة اللي لو لقيت لي أنت وبقيتك
+
+285
+00:21:35,800 --> 00:21:41,160
+تلعب عدى كل هذه اللي بيبقى الـ style colorless
+
+286
+00:21:41,160 --> 00:21:45,800
+بيكون ممتاز ما أنا أحكي إن الـ MIC تبقى صغيرة جدا
+
+287
+00:21:45,800 --> 00:21:51,960
+طبعا إيه اسم الـ growth dilution؟ وهو اسم الموضوع بس
+
+288
+00:21:51,960 --> 00:21:55,320
+عشان ... عشان اشتغلناها في wells صغيرة بيسموها
+
+289
+00:21:55,320 --> 00:21:59,620
+micro growth dilution micro growth dilution إيه
+
+290
+00:21:59,620 --> 00:22:02,360
+كان الطريقة اللي قصدناها فيها الأهل؟ عرفنا disk
+
+291
+00:22:02,360 --> 00:22:04,700
+diffusion واخدوا بالك الـ disk diffusion هذي هي
+
+292
+00:22:04,700 --> 00:22:06,660
+الأهم على الإطلاق لكوا في التحيات الطبي يعني أن هي
+
+293
+00:22:06,660 --> 00:22:09,360
+اللي بتشتغلوا فيها الـ sensitivity testing الـ
+
+294
+00:22:09,360 --> 00:22:13,000
+sensitivity testing وأنا صحي بحب أستغلها وأوسع
+
+295
+00:22:13,000 --> 00:22:17,840
+منها لك الطلاب في كيفية استخدام أو استغلال هذه
+
+296
+00:22:17,840 --> 00:22:18,200
+الطريقة
+
+297
+00:22:21,540 --> 00:22:25,700
+وجيبولنا سائل
+
+298
+00:22:25,700 --> 00:22:31,800
+مطهر وجيبولنا بنشوف الفعالية تبعتهممكن أستخدم نفس
+
+299
+00:22:31,800 --> 00:22:35,380
+الطريقة بدون antibiotic discs بس للأسف مش هيعطوني
+
+300
+00:22:35,380 --> 00:22:40,800
+هم دسكات فيها المطهر ولا القيمة هم هيعطوني مطهر
+
+301
+00:22:40,800 --> 00:22:44,600
+سائل صح ولا لأ، إيش ممكن أعمل أنا؟ يا بكمل أندي
+
+302
+00:22:44,600 --> 00:22:49,620
+filter paper disc معقمين وبدمسهم بكمية محددة وبحطهم
+
+303
+00:22:49,620 --> 00:22:53,900
+زي ما أنا حاطب دسكات دولار أو بطريقة ثانية احنا
+
+304
+00:22:53,900 --> 00:22:57,200
+بصرا على البكتيريا بطبقها، بتعرف الـ tip تبعت الـ ...
+
+305
+00:22:57,200 --> 00:23:00,140
+الـ ... الـ yellow tips والـ blue tips، بنجيب الـ blue 
+
+306
+00:23:00,140 --> 00:23:05,220
+tip وبنفقه بالطبق خمس أو ست مواد وبننيدل ...
+
+307
+00:23:05,220 --> 00:23:10,000
+بنفرغ الـ ... الأجار لما نبقى بصير فيه حفر، حفر،
+
+308
+00:23:10,000 --> 00:23:14,720
+اسمه well ... well diffusion، بلغ مدرسك صارش لقاعد
+
+309
+00:23:14,720 --> 00:23:19,700
+حفرة well diffusion بضيف فيها الكمية المطلوبة للفحص 
+
+310
+00:23:19,700 --> 00:23:22,740
+وبضيعها من خلالها برضه وكثير من زميلات كهوئي
+
+311
+00:23:22,740 --> 00:23:25,480
+اللي عملوا دراسات على antimicrobial activity لبعض
+
+312
+00:23:25,480 --> 00:23:28,800
+النباتات أو مشتقات النباتات عملوا بهذه الطريقة و
+
+313
+00:23:28,800 --> 00:23:32,400
+حصلوا نتائج ممتازة جدا إذا ... ببنقدر من خلال ...
+
+314
+00:23:32,400 --> 00:23:35,600
+طبعا الـ ... الـ ... عملية الـ antimicrobial testing
+
+315
+00:23:35,600 --> 00:23:43,090
+مهمة جدا أعطيكوا مثال واحد أعطينا antibiotic بنشوف
+
+316
+00:23:43,090 --> 00:23:47,210
+الـ antibiotic وصل في دسمه بتركيز كافي لقتل
+
+317
+00:23:47,210 --> 00:23:50,130
+البكتيريا اللي أخدنا منه عينة دم فصلناها السيرام،
+
+318
+00:23:50,130 --> 00:23:53,350
+الآن المفروض أن الـ antibiotic هو السيرام، بياخد
+
+319
+00:23:53,350 --> 00:23:58,380
+السيرام بعد report testing بالطريقة هذه إمكانية
+
+320
+00:23:58,380 --> 00:24:02,860
+توظيف هذه الفكرة عالية جدا في مجالات متنمو لكن في
+
+321
+00:24:02,860 --> 00:24:06,380
+الآخر كلها تتلخص باني أشوف مدى antimicrobial
+
+322
+00:24:06,380 --> 00:24:09,820
+activity لمركب على جزء أكبر من الشغل اللي نشتغله
+
+323
+00:24:09,820 --> 00:24:12,620
+بالكيميا مع الكيميا هو اللي ناخد مركبته ونبحث عنه
+
+324
+00:24:12,620 --> 00:24:15,960
+معاهم بالطريقة هذه اللي جينا في zone of inhibition
+
+325
+00:24:15,960 --> 00:24:20,480
+بنعمل الـ micro process dilution
+
+326
+00:24:27,470 --> 00:24:31,150
+دي جزء الثاني من الموضوع المتعلق بالـ testing اللي 
+
+327
+00:24:31,150 --> 00:24:34,230
+هو الـ resistance الـ resistance وأنا بقى بحملكوا
+
+328
+00:24:34,230 --> 00:24:37,150
+أمانة يعني أمانة إنه المعلومات اللي بناخدها اليوم
+
+329
+00:24:37,150 --> 00:24:41,550
+أنها بنشرها فيها بالناس في المجتمع لأنه إلها أهمية
+
+330
+00:24:41,550 --> 00:24:47,310
+كبيرة في يعني المحاولة تخفض هذه الفائرة في مجتمع
+
+331
+00:24:47,310 --> 00:24:54,830
+الفلسطيني أي تغيير في الـ DNA تبع البكتيريا بنسميه
+
+332
+00:24:54,830 --> 00:24:56,650
+mutation وغالبا الـ resistance
+
+333
+00:24:59,760 --> 00:25:05,820
+أو إنها Induced، Induced يعني بفعل بفعل، Mechanism
+
+334
+00:25:05,820 --> 00:25:10,960
+of Antibiotic Resistance، الآن مختلفة واضحة، نتكلم
+
+335
+00:25:10,960 --> 00:25:15,960
+عن أن الفكرة العامة أنه mutation، لكن كيف بتصير؟
+
+336
+00:25:15,960 --> 00:25:19,720
+بكتيريا عندها إنزيم قادر على تكسير، قادر على تكسير
+
+337
+00:25:19,720 --> 00:25:21,260
+اللي هو الـ Antibiotic،
+
+338
+00:25:25,520 --> 00:25:28,940
+هو تتكلم عن نموذج لو بدنا ندخل في تقاط أصيل كل
+
+339
+00:25:28,940 --> 00:25:32,620
+الانتيبايتكس يلو ميكانيزم حتى في تباون إنزيمات
+
+340
+00:25:32,620 --> 00:25:36,600
+مختلفة enzymatic destruction of the drug واخدنا
+
+341
+00:25:36,600 --> 00:25:39,820
+مثلها البرنامج الأساسي preventing of penetrating
+
+342
+00:25:39,820 --> 00:25:43,860
+of drug يعني الـ drug بده يدخل ويعدي لأن حجمه صغير
+
+343
+00:25:43,860 --> 00:25:47,080
+بكل سهولة وسلاسة وكانت الـ protein بيقول اتفضل
+
+344
+00:25:47,080 --> 00:25:50,800
+ودخلوا جوه لو صار في mutation في الـ target اللي
+
+345
+00:25:50,800 --> 00:25:54,720
+براني تبع الـ entrance مش هدفه دلوقتي و دلوقت هو الـ
+
+346
+00:25:54,720 --> 00:25:58,960
+antibiotic useless ثالثا أنه يغير من الـ target
+
+347
+00:25:58,960 --> 00:26:02,940
+صحيح قلتلكوا أنا بيرتبط بالـ 50S لما يرتبط بالـ 50S
+
+348
+00:26:02,940 --> 00:26:06,540
+بيمنع الـ translocation فيقول بيرتبط الـ 50S ما هو
+
+349
+00:26:06,540 --> 00:26:10,940
+له target للارتباط إذا البكتيريا عملت mutation في
+
+350
+00:26:10,940 --> 00:26:14,620
+هذا الـ target خدوا بركوا mutation في target لم 
+
+351
+00:26:14,620 --> 00:26:19,220
+يؤثر على الوظيفة يعني أكرد الباب ده اللي أكرد باب،
+
+352
+00:26:19,220 --> 00:26:22,980
+تمام؟ لكن الاختلاف إن دياجك بطلت تسمع للـ
+
+353
+00:26:22,980 --> 00:26:26,220
+antibiotic إنه يبقى بطفي، هذا الكلب حكيناه قبلين،
+
+354
+00:26:26,220 --> 00:26:29,980
+الرابع عالمي زي كتير اسمها rapid ejection of the
+
+355
+00:26:29,980 --> 00:26:33,120
+tractor، مش هنفهم اللي مابيقنها؟ فضلوا يابانها، مع
+
+356
+00:26:33,120 --> 00:26:37,440
+السلامة بتاخدوا الدب من الباب هذا، تمام؟ وبيطلعوا
+
+357
+00:26:37,440 --> 00:26:40,200
+من الباب ثاني وكأنه صار فيه انديو transport
+
+358
+00:26:40,200 --> 00:26:44,840
+system أنّه بيسموه rapid bombing أو rapid ejection
+
+359
+00:26:44,840 --> 00:26:48,340
+efflux bomb بيسموها efflux يعني اللي هي الدخل
+
+360
+00:26:48,340 --> 00:26:53,320
+للخارج، efflux bomb الأهم أننا نسترجع المعلومات
+
+361
+00:26:53,320 --> 00:26:56,340
+تبعتنا اللي كانت كلها معاها الـ gene transfer خدوا
+
+362
+00:26:56,340 --> 00:27:01,240
+بالكم الأم تنقل لبنتها للجينات، إيش بنسمي هذا نقل
+
+363
+00:27:01,240 --> 00:27:02,420
+من الجينات؟
+
+364
+00:27:16,120 --> 00:27:18,560
+انتقلت للـ تنتال
+
+365
+00:27:24,910 --> 00:27:27,230
+للـ horizontal gene transfer اللي درسته أنتو
+
+366
+00:27:27,230 --> 00:27:31,530
+ثلاثة إيش هما؟ conjugation، transformation و
+
+367
+00:27:31,530 --> 00:27:36,170
+transduction هدول أهم ثلاثة عناصر لنقل الـ
+
+368
+00:27:36,170 --> 00:27:39,150
+resistance من بكتيريا إلى بكتيريا أخرى، فهذه نقطة
+
+369
+00:27:39,150 --> 00:27:43,070
+كتير مهمة، الآن يجب أن نسترجعها ونتذكر كيف تنتقل
+
+370
+00:27:43,070 --> 00:27:49,430
+هذه الأشياء طيب كيف تنشأ بعض الـ resistance؟ أنا
+
+371
+00:27:49,430 --> 00:27:51,890
+ممكن أرئي في واحد بشكل مبسط وقلت لو في على
+
+372
+00:27:51,890 --> 00:27:56,760
+الطاولة عندي مليون خلية كلهم sensitive للبنسالين
+
+373
+00:27:56,760 --> 00:28:00,960
+إلا واحد هذه الواحدة المسكينة ... مسكينة بين
+
+374
+00:28:00,960 --> 00:28:04,680
+المليار أو المليون هذه المنافسة التي بعتها على
+
+375
+00:28:04,680 --> 00:28:11,240
+الغذاء مش سهلة ويمكن أن تموت قبل البكتيريا الحساسة 
+
+376
+00:28:11,240 --> 00:28:17,320
+لكن لو أنا استخدمت البنسلين ف أنا اللي اخترتها
+
+377
+00:28:17,320 --> 00:28:22,640
+باستخدام البنسلين مواتت كل شيء و خلتها هي و كوني
+
+378
+00:28:22,640 --> 00:28:26,740
+خلتها هي تمام فعطيت المساحة اللي جاب محتلها
+
+379
+00:28:26,740 --> 00:28:30,720
+المليارة و المليون و الإضاءة و ال environment كلها
+
+380
+00:28:30,720 --> 00:28:31,440
+لهذا البكتيري
+
+381
+00:28:43,900 --> 00:28:48,180
+مش هنقول إن أصلا الفطرية بتنتج antibiotics و
+
+382
+00:28:48,180 --> 00:28:52,220
+البكتيريا بتنتج antibiotics لكن كانت تحدث بمعدلات
+
+383
+00:28:52,220 --> 00:28:56,020
+ضائلة جدا جدا لما كانت العملية natural لما صار
+
+384
+00:28:56,020 --> 00:28:59,780
+الإنسان .. و تذكروا .. الإنسان بيستعملون قطف
+
+385
+00:28:59,780 --> 00:29:03,180
+الثمار في المعارف الأخرى محدد ما عرفنا الظاهر أن
+
+386
+00:29:03,180 --> 00:29:06,160
+ال antibiotic أخذناهم و قلنا نستخدمهم بشكل كبير
+
+387
+00:29:06,160 --> 00:29:11,480
+اسمعوا بعد مبادرة .. مبادرة سوء استخدام الموضوعات
+
+388
+00:29:11,480 --> 00:29:15,940
+الحيوية و جيسوه على مجتمع ان هل الموضوع اللي موجودة
+
+389
+00:29:15,940 --> 00:29:18,720
+عندكوا في البرموجودة اللي موجودة؟ أنا قعدت كده و
+
+390
+00:29:18,720 --> 00:29:22,180
+درست في كلية كتب أذن سنوات ل media global و كنت
+
+391
+00:29:22,180 --> 00:29:27,500
+أسالهم السؤال مين طبيب المنزل؟ طبيب المنزل يعني
+
+392
+00:29:27,500 --> 00:29:31,540
+مان أخر، مين اللي بيكون بالمهمة يعالج الأطفال؟ فيه
+
+393
+00:29:31,540 --> 00:29:35,680
+طبعا أبسط ناس .. أبسط ناس اللي .. اللي بـ طبيب المنزل
+
+394
+00:29:35,680 --> 00:29:40,560
+اللي بيجي على الدكتورة، هذا أمتاز، perfect الانصاب
+
+395
+00:29:40,560 --> 00:29:46,000
+المتعلمين يعني أما مدرسة أما مدرسة فبقى و بتقول
+
+396
+00:29:46,000 --> 00:29:50,120
+الطالب الطالب الطب الآن يعني صادقة في وصفها بتقول
+
+397
+00:29:50,120 --> 00:29:53,960
+أمي طول عمرها اللي بتشترينا الأدوية و اللي بتودينا
+
+398
+00:29:53,960 --> 00:29:56,860
+أو ديونا من الصيدلي بتروح الصيدلي بتقوله هات
+
+399
+00:29:56,860 --> 00:30:02,560
+اعطيني ده للوازي اعطيني ده و أنا كده هذا اسمه
+
+400
+00:30:02,560 --> 00:30:05,960
+misuse لـ الـ الـ الـ الـ 
+
+401
+00:30:11,680 --> 00:30:14,720
+using outdated .. outdated دي يعني إبتدالية تبعها
+
+402
+00:30:14,720 --> 00:30:19,220
+منتهي weakened antibiotics طبعا لما بنتهي صلاحيتها
+
+403
+00:30:19,220 --> 00:30:26,110
+أو قساء تخزينها ال antibiotic تضعف و بالتالي بناخدها
+
+404
+00:30:26,110 --> 00:30:30,430
+احنا هي بناخدها لـ 150 ملي جرام عصير المثال أو من
+
+405
+00:30:30,430 --> 00:30:34,050
+500 و واقع نعمل active compound منها لأن بكتر واحد
+
+406
+00:30:34,050 --> 00:30:39,810
+من 100 فبالتالي هتبعرض البكتيريا لـ ثرائيز منخفضة
+
+407
+00:30:39,810 --> 00:30:43,650
+فإن كانت ال resistance مش لان اعلى using
+
+408
+00:30:43,650 --> 00:30:46,410
+antibiotics for the common cold and other
+
+409
+00:30:46,410 --> 00:30:47,010
+inappropriate
+
+410
+00:30:49,920 --> 00:30:54,180
+Does not require antibiotic treatment. They are
+
+411
+00:30:54,180 --> 00:30:58,080
+viral and viruses do not respond to antibiotics.
+
+412
+00:30:59,040 --> 00:31:04,820
+لكن هتقنع الناس إنه يروح على طبيب و يمشي على
+
+413
+00:31:04,820 --> 00:31:10,390
+الفلوس تعمال و مايكتبوش antibiotic للأسف احنا
+
+414
+00:31:10,390 --> 00:31:13,910
+بنشكل الضغط على الأطباء إنه يكتبونا ال antibiotic
+
+415
+00:31:13,910 --> 00:31:19,010
+بس النافذون الأطباء يكونوا وعين بدرجة كبيرة و
+
+416
+00:31:19,010 --> 00:31:22,810
+يرفضوا هذا الكلام لكن للأسف بيستجيبوا أيضا للـ
+
+417
+00:31:22,810 --> 00:31:30,020
+use of antibiotics in animal feed قبل أقل من 6 أشهر
+
+418
+00:31:30,020 --> 00:31:33,300
+كان في ريبورتاج صحفي طلع على اللي .. اللي أنا في
+
+419
+00:31:33,300 --> 00:31:36,520
+فلسطين هناك إنه استخدام العقاقي و المناطق الحيوية
+
+420
+00:31:36,520 --> 00:31:39,280
+في الدواجن و المسا كبيرة احنا كنا في تلك الفترة
+
+421
+00:31:39,280 --> 00:31:43,500
+بنعمل دراسة على هذا الموضوع فكلمونا في وزارة
+
+422
+00:31:43,500 --> 00:31:46,260
+الزراعة وجالي أخواننا يعني قابلونا دورة ضيحة من
+
+423
+00:31:46,260 --> 00:31:49,060
+غير ما كان عندهم أي .. أي .. أي شيء عشان كنتوا ال
+
+424
+00:31:49,060 --> 00:31:52,300
+الـ الـ الـ المعلومات فاحنا عندنا معلومات علمية
+
+425
+00:31:52,300 --> 00:31:56,860
+دقيقة بفحصات وكميات وأنواع من ال antibiotic فيه لكن
+
+426
+00:31:56,860 --> 00:31:59,500
+مش بالصورة اللي .. اللي .. اللي نشرت في ال
+
+427
+00:31:59,500 --> 00:32:04,140
+laboratory لما أنا بدي أـ أـ اشتري دجاج أو لحم
+
+428
+00:32:04,140 --> 00:32:07,460
+أو اكبار دلوقتى دجاج أنا بدي آكل لحم مابدي
+
+429
+00:32:07,460 --> 00:32:10,440
+antibiotic مابدي antibiotic لأن ال antibiotic قد
+
+430
+00:32:10,440 --> 00:32:14,600
+يسبب لي أنا side effects منها حساسية ممكن يسبب لي
+
+431
+00:32:14,600 --> 00:32:18,460
+abnormalities في ال normal flora صحيح؟ يبقى أنا
+
+432
+00:32:18,460 --> 00:32:22,560
+بحتاج دجاج بدون antibiotic أو لحم بدون antibiotics
+
+433
+00:32:22,560 --> 00:32:24,380
+للأسف
+
+434
+00:32:27,920 --> 00:32:31,260
+المزارعين نظرتهم للدواجن مش زي نظرتنا للدواجن زي
+
+435
+00:32:31,260 --> 00:32:33,780
+ما زواجين تاكسي كده مش هوك زواجين تاكسين مش هواك
+
+436
+00:32:33,780 --> 00:32:37,360
+تمشي على الأرض بقولها .. بقولها إنك رهيبة خالية
+
+437
+00:32:37,360 --> 00:32:41,600
+بقولها أنا اتنين شكل فالدواجن
+
+438
+00:32:41,600 --> 00:32:45,160
+المربي الدواجن بيشوفوا الدواجن بطريقة مختلفة
+
+439
+00:32:45,160 --> 00:32:50,290
+جاتين يشتروا علف وزن جدّيش تحوّل لوزن دجاج، هما
+
+440
+00:32:50,290 --> 00:32:53,630
+بيسموه conversion rate، جدّيش سبعة ميل تحوّل العرف
+
+441
+00:32:53,630 --> 00:32:57,450
+الهقل، العرف أرخص و الدجاج أقل، فلو كان كيلو
+
+442
+00:32:57,450 --> 00:33:01,190
+بيجيبله كيلو بيكون perfect، طبعا هذا الكلام مستحيل
+
+443
+00:33:02,050 --> 00:33:06,370
+فلا يعني يراعوا
+
+444
+00:33:06,370 --> 00:33:12,090
+أنه في صحة بالأدمين على المحق في هذا الموضوع، فمش
+
+445
+00:33:12,090 --> 00:33:15,290
+بس بيستخدموا ال antibiotic في الهلاج، أصبحوا كمان
+
+446
+00:33:15,290 --> 00:33:18,490
+يستخدموه كـ animal feed، مش animal feed
+
+447
+00:33:34,940 --> 00:33:40,740
+النقطة الأخيرة was failure to complete the
+
+448
+00:33:40,740 --> 00:33:41,800
+prescribed regimen
+
+449
+00:33:50,790 --> 00:33:54,550
+المشكلة .. المشكلة بقى إنه مش برتبة كمان، طبعا هو
+
+450
+00:33:54,550 --> 00:33:58,950
+غلط إن احنا نوقف، لازم إنه نكمل ال course، لآخر
+
+451
+00:33:58,950 --> 00:34:04,270
+يعني أكاسولة أو أخر حلقة whatsoever الشكل، الدلال،
+
+452
+00:34:04,270 --> 00:34:06,650
+لآخر جرعة، لآخر جرعة، لآخر جرعة، لآخر جرعة، لآخر
+
+453
+00:34:06,650 --> 00:34:07,070
+جرعة، لآخر جرعة، لآخر جرعة، لآخر جرعة، لآخر جرعة،
+
+454
+00:34:07,070 --> 00:34:11,170
+لآخر جرعة، لآخر جرعة، لأخر جرعة، لأخر جرعة، لأخر
+
+455
+00:34:11,170 --> 00:34:12,890
+جرعة، لأخر جرعة، لأخر جرعة، لأخر جرعة، لأخر جرعة،
+
+456
+00:34:12,890 --> 00:34:15,270
+لأخر جرعة، لأخر ج
+
+457
+00:34:19,760 --> 00:34:24,460
+اللي هو ال antibiotic من آخر، إذا في ممارسات
+
+458
+00:34:24,460 --> 00:34:29,760
+خاطئة، في ممارسات سيئة قد تساعد، قد تؤدي إلى زيادة
+
+459
+00:34:29,760 --> 00:34:36,220
+اللي هو الـ لكن أنا أريد أن أختم بنقطة مهمة جدا،
+
+460
+00:34:36,220 --> 00:34:42,740
+وهي إن هناك دور حكومي مفقود تماما في الرقابة على
+
+461
+00:34:42,740 --> 00:34:44,320
+صرف الأدب، لا في العقاب
+
+462
+00:34:52,950 --> 00:34:57,210
+لو احنا بعتنا طفل طوله بيصارش ال counter تبع
+
+463
+00:34:57,210 --> 00:34:59,650
+الصيدلية ومعاه meat shake اللي هو ورقة مكتوب فيها
+
+464
+00:34:59,650 --> 00:35:03,610
+اسم الدواء بيبعتوه الدواء بدون prescription ال
+
+465
+00:35:03,610 --> 00:35:06,790
+antibiotic are prescription drugs بمعنى إنه لا
+
+466
+00:35:06,790 --> 00:35:11,490
+تصرف إلا بوصفة طبية وهذا كله للأسف مين مجدرجون فيه
+
+467
+00:35:11,490 --> 00:35:16,150
+فقط الحكومة طلقاء تشريع و مراقبة هذا التشريع هذا
+
+468
+00:35:16,150 --> 00:35:22,590
+بحلم الأزمة هذا واحد رقم اثنين الانصياع لتعليمات
+
+469
+00:35:22,590 --> 00:35:28,710
+مندوبي الأدوية، يعني الطبيب بطل ياخد معلوماته من
+
+470
+00:35:28,710 --> 00:35:32,210
+الكتاب أو من الأبحاث أو من ال journal بياخد
+
+471
+00:35:32,210 --> 00:35:35,850
+معلوماته من مسوقي الأدوية، و بالتالي بقى رايش رجل
+
+472
+00:35:35,850 --> 00:35:43,760
+مبلغ هذا، ممنوع مندوب الأدوية يعطي الطبيب أدوية
+
+473
+00:35:43,760 --> 00:35:49,340
+حقيقية بيعطيه sample شكل منظر خارج مش الأهل بيعطيه
+
+474
+00:35:49,340 --> 00:35:53,100
+أهلا بيقولوا بكتابة بيقولوا تفضل هدية هدية هدية
+
+475
+00:35:53,100 --> 00:35:56,660
+عشواء يعني هدية عشواء و بالتالي بصيب الفئة اللي هو
+
+476
+00:35:56,660 --> 00:36:01,920
+عشان كل مرة يمول عليه المندوب يعطيه الأدوية الهدية
+
+477
+00:36:01,920 --> 00:36:07,150
+بدى يصرف أو يكتبره شتات من هذا أيضا كمان من ضمن
+
+478
+00:36:07,150 --> 00:36:10,330
+الأنظمة اللي الأصل إنها تتطور بحيث إنها تمنع هذه
+
+479
+00:36:10,330 --> 00:36:14,670
+الظاهر المرة القادمة إن شاء الله هنشوف كيف
+
+480
+00:36:14,670 --> 00:36:19,890
+التفاعلات الـ الـ antibiotic بع بعضها واحد بيزورك
+
+481
+00:36:19,890 --> 00:36:23,470
+من قدام و واحد بيخرج من ورا مكانك سبب، مصبوط؟
+
+482
+00:36:23,470 --> 00:36:27,690
+مكانك سبب، و إن شاء الله المرة القادمة بسم الله
+
+483
+00:36:27,690 --> 00:36:30,810
+الرحمن الرحيم السلام عليكم ورحمة الله وبركاته
+
+484
+00:36:30,810 --> 00:36:33,230
+اليوم سنبدأ محاضرة ال essential medical
+
+485
+00:36:33,230 --> 00:36:37,430
+microbiology بأسلوب غير عالمي معتاد اليوم أول
+
+486
+00:36:37,430 --> 00:36:41,990
+شخصية أهلا وسهلا بما نتشرف في لقائهم أهلا وسهلا
+
+487
+00:36:41,990 --> 00:36:46,590
+بزميلاتي وأصدقائي وأحبابي أهلا وسهلا بكم جميعا
+
+488
+00:36:46,590 --> 00:36:49,510
+اليوم
+
+489
+00:36:52,870 --> 00:36:57,550
+اليوم سنكشف لكم عن إحدى قنابل هذه الموصلة قضية
+
+490
+00:36:57,550 --> 00:37:01,510
+أثارت الجدل منذ زمن .. منذ زمن النبي العدنان ..
+
+491
+00:37:01,510 --> 00:37:05,590
+منذ زمن النبي العدنان عليه الصلاة والسلام حيث إنه
+
+492
+00:37:05,590 --> 00:37:09,130
+كان يشتكي من آلام تجعله يمكث ما بين اليوم واليومين
+
+493
+00:37:09,130 --> 00:37:13,290
+في بيته ولا يخرج وأنه عن ابن عباس رضي الله عنه قال
+
+494
+00:37:13,290 --> 00:37:17,730
+أنه كان يعصب عصبة على رأسه وأنه في حادث شدري قال
+
+495
+00:37:17,730 --> 00:37:22,310
+وفي حادث صحيح أنه قال في أثناء مرض موته ورأساه وكان
+
+496
+00:37:22,310 --> 00:37:26,130
+يعصب عصابة على رأسه وذكر أنه عليه الصلاة والسلام
+
+497
+00:37:26,130 --> 00:37:30,650
+ما اشتكى إليه أحد من رأسه إلا وقال له احتجم وما اشتكى
+
+498
+00:37:30,650 --> 00:37:34,810
+إليه أحد من ألمت في قدميه إلا وقال له تضب الحناء،
+
+499
+00:37:34,810 --> 00:37:38,990
+اليوم نزف لكم إحدى قنابل نفسي micro organism صغيرة
+
+500
+00:37:38,990 --> 00:37:43,450
+استطاعت أن تحل تلك القضية المثار عليها الجدال صعبت 
+
+501
+00:37:43,450 --> 00:37:47,610
+على أهم الـ antibiotic من حلها micro organism أراحت
+
+502
+00:37:47,610 --> 00:37:52,530
+و ناس طالما اشتكت من آلام شديدة لساعة طول لا لألام 
+
+503
+00:37:52,530 --> 00:37:55,410
+صداع الشقيقة بعد اليوم no pain of migraine
+
+504
+00:37:55,410 --> 00:37:59,190
+headaches after today ماهو صداع الشقيقة صداع
+
+505
+00:37:59,190 --> 00:38:02,450
+الشقيقة هذه الكلمة مشتقة من اللغة اليونانية وهي
+
+506
+00:38:02,450 --> 00:38:07,110
+التي تعني آلام في الرأسو أيضا مشتقة من كلمة hemi
+
+507
+00:38:07,110 --> 00:38:11,910
+التي تعني نصف و the carry on التي تعني جمجمة أما من
+
+508
+00:38:11,910 --> 00:38:15,930
+ناحية الطبية فهي tuat فهي تعني آلام شديدة تصبح في
+
+509
+00:38:15,930 --> 00:38:19,790
+نصف من الرأس الأيمن أو الأيسر مصاحبة بمجموعة من
+
+510
+00:38:19,790 --> 00:38:25,010
+الأعراضو من أهم الأعراض التي سنذكرها لكم سوية ما
+
+511
+00:38:25,010 --> 00:38:28,370
+يشعر به المريض في مرض الـ migraine headaches هي
+
+512
+00:38:28,370 --> 00:38:32,270
+التقيؤ و حالة من الـ vomiting و الغثيان كذلك يصبح
+
+513
+00:38:32,270 --> 00:38:36,110
+عنده ازدواجية في الرؤية كرؤية أن يرى الشيء كـ zig
+
+514
+00:38:36,110 --> 00:38:40,130
+zag أن يراه على هيئة شيئين متقابلين أو شيئين فوق
+
+515
+00:38:40,130 --> 00:38:44,440
+بعضهم البعض كما أنه يشتكي من Sensitive of the light
+
+516
+00:38:44,440 --> 00:38:48,820
+and fawn بما يسمى بالفوتوفوبيا and فونوفوبيا إضافة
+
+517
+00:38:48,820 --> 00:38:52,740
+إلى أعراض كثيرة قد يشتك منها سأطلب قعد السلام على
+
+518
+00:38:52,740 --> 00:38:56,200
+أسباب هذا المرض ومن أهم هذه الأسباب العوامل
+
+519
+00:38:56,200 --> 00:39:01,570
+الوراثية إلى أهم .. أهم من ذلك، البيئة المحيطة،
+
+520
+00:39:01,570 --> 00:39:05,270
+حيث أن البيئة المحيطة تؤثر على نفسية الشخص بالسلب
+
+521
+00:39:05,270 --> 00:39:08,770
+أو الإيجاب، حيث تؤثر عليه بالسلب عندما يكون الشخص
+
+522
+00:39:08,770 --> 00:39:12,930
+في حالة اكتئاب أو أن أصبح لديه ظروف تجعل الشخص في
+
+523
+00:39:12,930 --> 00:39:17,350
+حالة نفسية سيئة، فيصبح من .. من أكثر عرضة لهذا
+
+524
+00:39:17,350 --> 00:39:22,570
+المرض بالإضافة إلى سبب مهم وجود خلل في جهاز العصبية
+
+525
+00:39:22,570 --> 00:39:26,610
+و خاصة في الـ answer يجعل الشخص معرض بشكل كبير إلى
+
+526
+00:39:26,610 --> 00:39:30,170
+الإصابة بهذا المرض بعض الدراسات كما تبين الصورة
+
+527
+00:39:30,170 --> 00:39:33,670
+بعض الدراسات قامت فيها أحد الأشخاص في .. أحد
+
+528
+00:39:33,670 --> 00:39:39,110
+الطلبة في جامعة عدن الـ USA على مجموعة من الناس تبين
+
+529
+00:39:39,110 --> 00:39:44,690
+أن تقريبا 6% من الرجال مصابين بهذا المرض مقابل 18%
+
+530
+00:39:44,690 --> 00:39:48,110
+من النساء وهذا تدل على أن النساء أكثر عرضة
+
+531
+00:39:48,110 --> 00:39:52,490
+بالإصابة بهذا المرض لأن المرأة كائن حساس أكثر من
+
+532
+00:39:52,490 --> 00:39:57,880
+الرجل أما الـ treatments للماجين of headaches أولا
+
+533
+00:39:57,880 --> 00:40:01,020
+نبدأ معاكم بالـ propranolol الـ propranolol هو عبارة
+
+534
+00:40:01,020 --> 00:40:04,100
+عن دواء يعمل على الـ tokens of the beta الـ
+
+535
+00:40:04,100 --> 00:40:08,680
+baricitamol هذا دواء مضاد أو مسكن عام الـ Evaliant
+
+536
+00:40:08,680 --> 00:40:12,380
+و هو مضاد الاكتئاب و الـ Valerobic Acid و هو مضاد
+
+537
+00:40:12,380 --> 00:40:17,020
+التشنج و أخيرا الـ veramil و هو يعني دواء يعمل على
+
+538
+00:40:17,020 --> 00:40:20,240
+الـ cutting or blocking of the calcium لم نستطع
+
+539
+00:40:20,240 --> 00:40:23,820
+حصر كافة الأدوية المستخدمة لعلاج هذا المرض لكن
+
+540
+00:40:23,820 --> 00:40:27,900
+ذكرنا بما يكفي حتى تتوضح لذاكم الصورة لكن الاكتئاب
+
+541
+00:40:27,900 --> 00:40:32,600
+من هذه الأدوية تجعل الشخص معرض للإدمان مما يؤدي
+
+542
+00:40:32,600 --> 00:40:36,830
+ذلك إن جعل جهاز المناعة دليل اتجاه هذه الأدوية و
+
+543
+00:40:36,830 --> 00:40:40,550
+هناك نوع من أنواع الـ micro organism استطاعت أن تحلل
+
+544
+00:40:40,550 --> 00:40:45,610
+لنا أحد أسباب ذلك المرض و هو مختص في الـ gestric
+
+545
+00:40:45,610 --> 00:40:49,290
+tract و هو في الجهاز الهضمي بشكل أخص و هي
+
+546
+00:40:49,290 --> 00:40:52,490
+lactobacillus acetophilus lactobacillus
+
+547
+00:40:52,490 --> 00:40:55,930
+acetophilus كما نعلم و هي بكتيريا لبن الرائد تعد
+
+548
+00:40:55,930 --> 00:41:00,010
+gram positive bacteria fragmented sugar and lactic
+
+549
+00:41:00,010 --> 00:41:05,370
+acids تعيش في وسط بيئة يكون منخفض كما أن الـ optimum
+
+550
+00:41:05,370 --> 00:41:08,730
+growth ده هي عبارة عن 37 درجة دزية، بالإضافة إلى
+
+551
+00:41:08,730 --> 00:41:11,710
+أن الـ lactobacillus أسودوفلوس تعاد friendly
+
+552
+00:41:11,710 --> 00:41:16,750
+للبكتيريا كـ normal flora تمام، normal flora تمام،
+
+553
+00:41:16,750 --> 00:41:20,110
+حيث أنها تعمل على إنتاج الـ vitamin K للمعدة
+
+554
+00:41:20,110 --> 00:41:24,490
+والكتاز بالإضافة إلى ما قلته نرميل أن الـ
+
+555
+00:41:24,490 --> 00:41:27,650
+lactobacillus acidophilus لها القدرة على تقلد
+
+556
+00:41:27,650 --> 00:41:32,550
+مستوى التوكسن أو النازل الذي وجد فيه الدم
+
+557
+00:41:32,550 --> 00:41:38,890
+بالإضافة إلى أنها تقوم بتقليل من كمية الـ pathogen
+
+558
+00:41:38,890 --> 00:41:43,510
+microorganism التي توجد في جسم الإنسان علاقة على
+
+559
+00:41:43,510 --> 00:41:47,390
+ذلك لها القدرة إلى عمل fermentation للمنتجات
+
+560
+00:41:47,390 --> 00:41:51,330
+اللبانية أو خاصة اليوغراد بالإضافة لبعض من أنواع
+
+561
+00:41:51,330 --> 00:41:56,080
+الأشياء الـ Helicobacter pylori ماهي سبب ذكري لهذه
+
+562
+00:41:56,080 --> 00:41:58,940
+البكتيريا؟ هذه البكتيريا بالأخص يا نور هي نوع من
+
+563
+00:41:58,940 --> 00:42:02,080
+أنواع البكتيريا المسببة للهيلسرز الهيلسرز هي
+
+564
+00:42:02,080 --> 00:42:05,980
+القرحة قرحة المعدة وكما ذكرنا مسبقا أن قرحة
+
+565
+00:42:05,980 --> 00:42:09,980
+المعدة هي أحد الأسباب التي تسبب صداع نسبي أو ما
+
+566
+00:42:09,980 --> 00:42:13,220
+يسمى بالـ migraine وهو صداع الشقيقة الـ Helicobacter
+
+567
+00:42:13,220 --> 00:42:16,980
+pylori gram negative helic شهدت وكما نرى جانبا
+
+568
+00:42:16,980 --> 00:42:21,000
+أنها تمتلك للفلاجلة التي تساعدها على الحركة حيث
+
+569
+00:42:21,000 --> 00:42:25,800
+أنها تسبب المرض كذلك للإنسان يعني تقسم إلى نوعين
+
+570
+00:42:25,800 --> 00:42:28,480
+من المرض وهي الـ chronic infections and acute
+
+571
+00:42:28,480 --> 00:42:31,760
+infections علاوة على ذلك فإنها لا تسبب فقط لا
+
+572
+00:42:31,760 --> 00:42:35,340
+تكتفي بتسبب المرض للإنسان إنها تتعالى أيضا و تسبب
+
+573
+00:42:35,340 --> 00:42:39,200
+المرض كذلك للـ animals كما ذكرت أنها تسبب المرض الـ
+
+574
+00:42:39,200 --> 00:42:42,160
+acute .. تسبب acute infection بالإضافة لـ chronic
+
+575
+00:42:42,160 --> 00:42:45,080
+infection الـ acute infection من أهم الأمراض التي
+
+576
+00:42:45,080 --> 00:42:48,600
+تتقوم بها أن الـ acute .. gastric acute وقد تكون
+
+577
+00:42:48,600 --> 00:42:52,480
+مصاحبة لها بمجموعة من الأعراض منها abdominal pain
+
+578
+00:42:52,480 --> 00:42:56,440
+بالإضافة لصداع في الرأس مع الغثيان قد يحدث في بعض
+
+579
+00:42:56,440 --> 00:43:00,740
+الأحيان غثيان تقيؤ كمان أنه في بعض الأحيان تقوم
+
+580
+00:43:00,740 --> 00:43:06,020
+بالـ .. الـ .. الـ stool بكل لونه black color و أيضا
+
+581
+00:43:06,020 --> 00:43:10,200
+تكون مصاحبة بأعراض أخرى و من أهمها وجود حرقة في
+
+582
+00:43:10,200 --> 00:43:15,010
+الجهاز الهضمي و خاصة في منطقة المعدة بالإضافة إلى
+
+583
+00:43:15,010 --> 00:43:18,670
+ذلك في مرض الـ chronic infection حيث تسببه هذه
+
+584
+00:43:18,670 --> 00:43:23,490
+البكتيريا من هذه الأمراض المزمنة الـ Peptic ulcer,
+
+585
+00:43:23,610 --> 00:43:27,780
+Gastritis, Gastric cancer بالإضافة إلى الـ lymphoma كما
+
+586
+00:43:27,780 --> 00:43:30,680
+أنها تعاد نوع من أنواع الـ micro-aerophilic أي أنها
+
+587
+00:43:30,680 --> 00:43:34,060
+تحتاج إلى وجود الأكسجين ولكن بتركيز منخفضة جدا
+
+588
+00:43:34,060 --> 00:43:38,540
+بالإضافة إلى ذلك فإنها تمتلك الـ hydrogenase تعمل على
+
+589
+00:43:38,540 --> 00:43:41,800
+إنتاج الطاقة، الطاقة تمتجها حتى تستطيع فيها الحركة
+
+590
+00:43:41,800 --> 00:43:45,940
+بواسطة الفلاجلة كما ذكرنا إضافة إلى ذلك فإنها تعمل
+
+591
+00:43:45,940 --> 00:43:49,360
+على أكسدة الهيدروجين حتى تعمل على إنتاج الـ
+
+592
+00:43:49,360 --> 00:43:53,140
+intestinal bacteria حتى نستطيع الكشف عنها نقوم
+
+593
+00:43:53,140 --> 00:43:57,120
+باستخدام مجموعة من الصبغات منها الـ gram stain و
+
+594
+00:43:57,120 --> 00:44:00,760
+خبرة عنها بالإضافة لـ الـ giemsa stain وهي إحدى الـ
+
+595
+00:44:00,760 --> 00:44:04,900
+vital stain المستخدمة لإستفاقض الخلايا الحية أيضا
+
+596
+00:44:04,900 --> 00:44:08,020
+الـ usain stain التي استخدمت في معامل الـ
+
+597
+00:44:08,020 --> 00:44:11,170
+microbiology و الـ hematology and the face contracts
+
+598
+00:44:11,170 --> 00:44:15,330
+microscopy وهو إحدى أنواع الميكروسكوب المستخدم
+
+599
+00:44:15,330 --> 00:44:17,950
+يتكشف عنه هو المعرف كذلك بـ microscope الـ invert
+
+600
+00:44:17,950 --> 00:44:21,430
+microscope enter why into the stomach cavity and
+
+601
+00:44:21,430 --> 00:44:25,530
+causing ulcers يا ترى كيف تسبب ليه بكتر ما يسمى
+
+602
+00:44:25,530 --> 00:44:29,350
+بقرحة المعدة؟ إليكم التالي كما أنا علمت فإن وسط
+
+603
+00:44:29,350 --> 00:44:33,650
+المعدة يعاد acidic environment أي أنه يتمتع بدرجة
+
+604
+00:44:33,650 --> 00:44:37,610
+حموضية عالية السبب في ذلك حتى تعمل على حماية هذا
+
+605
+00:44:37,610 --> 00:44:40,590
+العضو من وجود بكتيريا خطيرة يعني غير الـ normal
+
+606
+00:44:40,590 --> 00:44:43,990
+flora احنا متكلمة على البكتيريا الخطيرة حيث كذلك
+
+607
+00:44:43,990 --> 00:44:47,970
+يحتوي غشاء الـ stomach على ما يسمى بالـ mucus الـ
+
+608
+00:44:47,970 --> 00:44:51,070
+mucus هو نوع من أنواع البروتينات يعتبر gel أي
+
+609
+00:44:51,070 --> 00:44:54,430
+مادة هلامية لا تستطيع البكتيريا الحركة في داخله
+
+610
+00:44:54,430 --> 00:44:58,010
+يمسك في البكتيريا وكأنه يمنعها من الحركة ويتشبث
+
+611
+00:44:58,010 --> 00:45:01,870
+بها بأن لا تدخل إلى المعدة ولكن الـ heliobacter
+
+612
+00:45:02,550 --> 00:45:06,810
+pylori هو من أنواع البكتيريا الشرسة نوعا ما حيث
+
+613
+00:45:06,810 --> 00:45:10,130
+تعمل على تحويل الـ mucus gel إلى مادة سائلة حتى
+
+614
+00:45:10,130 --> 00:45:16,230
+تستطيع بوسط الفلجلة الحركة ومغادرة المكان الأكثر
+
+615
+00:45:16,230 --> 00:45:20,590
+سمية بالنسبة لها وذهب إلى المكان الأكثر حساسية لها
+
+616
+00:45:20,590 --> 00:45:24,730
+بالإضافة إلى ذلك فإن الـ Helicobacter pylori تقوم
+
+617
+00:45:24,730 --> 00:45:28,810
+باختراق جدار المعدة معناه أنها تختار جدار المعدة
+
+618
+00:45:28,810 --> 00:45:32,910
+أنها تغادر من منطقة الـ mucus تغادر من منطقة الـ
+
+619
+00:45:32,910 --> 00:45:36,430
+mucus و الـ gastric mucus gel حتى تصل لمنطقة الـ
+
+620
+00:45:36,430 --> 00:45:41,100
+epithelial cell تبدأ تسبب فجوات وخروق في داخل
+
+621
+00:45:41,100 --> 00:45:45,080
+الخلايا طب كما قلنا فإن هذا النوع من البكتيريا غير
+
+622
+00:45:45,080 --> 00:45:50,380
+محب لوسط الـ environment acidic فماذا ستفعل بعد
+
+623
+00:45:50,380 --> 00:45:54,500
+ذلك؟ ستقوم بإنتاج ما يسمى بمادة اليوريز، المادة
+
+624
+00:45:54,500 --> 00:45:58,320
+اليوريز ستقسم إلى ثاني أكسيد الكربون وتقسم إلى مادة
+
+625
+00:45:58,320 --> 00:46:02,200
+الامونيا الامونيا عبارة عن basic طب وجود الـ basic
+
+626
+00:46:02,200 --> 00:46:06,220
+مع وجود الـ acidic حياتنا الـ neutral فبالتالي فإن
+
+627
+00:46:06,220 --> 00:46:09,600
+هذا النوع من البكتيريا لها قدرة خارقة وذكية على 
+
+628
+00:46:09,600 --> 00:46:12,960
+استعمال الطرق البيو كيميائية لتحويل الوسط اللي 
+
+629
+00:46:12,960 --> 00:46:16,560
+حولها وحماية نفسها  حتى تصل إلى  مطابقة الـ epithelial
+
+630
+00:46:16,560 --> 00:46:21,060
+cell حتى تحول الوسط ما حولها إلى وسط الـ neutral pH
+
+631
+00:46:21,060 --> 00:46:25,660
+وبالتالي فإنها قد توجد إما على سطح الـ epithelial أو
+
+632
+00:46:25,660 --> 00:46:29,440
+في داخل الـ epithelial وهذه البكتيريا ليست بقدر ذكاء
+
+633
+00:46:29,440 --> 00:46:32,860
+بعض أنواع الـ antibiotic منها الـ homoverazole
+
+634
+00:46:32,860 --> 00:46:37,320
+بالإضافة لـ الـ amoxilane و الأشخاص الذين تتوفر
+
+635
+00:46:37,320 --> 00:46:41,480
+لديهم حساسية لـ البنسالين تقوم الدكاترة بإعطائهم الـ
+
+636
+00:46:41,480 --> 00:46:44,480
+amoxilane مع الـ metronidazole بالإضافة لـ
+
+637
+00:46:44,480 --> 00:46:45,720
+levofloxane
+
+638
+00:46:47,680 --> 00:46:52,200
+والآن ما أستطيع أن أُجمل لكم ما تحدثنا عنه مرض الـ
+
+639
+00:46:52,200 --> 00:46:55,760
+headaches، migraine ومرض الـ ulcers هو مرض القرحة
+
+640
+00:46:55,760 --> 00:46:59,940
+كما قلنا مسبقا أن مرض الـ ulcers وهو مرض القرحة أحد
+
+641
+00:46:59,940 --> 00:47:02,880
+الأسباب المؤدية لحدوث مرض الـ migraine اللي هو
+
+642
+00:47:02,880 --> 00:47:06,900
+أمراض في الجهاز الهضمي تسبب مرض صداع النصفي أو ما
+
+643
+00:47:06,900 --> 00:47:10,120
+يسمى بصداع الشقيقة ولكن مع أن Helicobacter pylori
+
+644
+00:47:10,120 --> 00:47:14,100
+استطاعت أن تحل عقدة ومشكلة هذا المرض اللي هو الـ
+
+645
+00:47:14,100 --> 00:47:19,340
+ulcers ومعالجة وعلاج بتاعه بعلاج السبب نستطيع حل
+
+646
+00:47:19,340 --> 00:47:22,960
+اللي هو السبب الأكبر اللي هو الـ migraine و كما
+
+647
+00:47:22,960 --> 00:47:26,700
+أثبتت الدراسات العلمية في جامعة ميلان في مدينة
+
+648
+00:47:26,700 --> 00:47:30,380
+إيطاليا حيث قامت بعمل إحصائية على مجموعة كبيرة من
+
+649
+00:47:30,380 --> 00:47:33,860
+الأشخاص المعانين من مرض الـ migraine  وجدوا أن
+
+650
+00:47:33,860 --> 00:47:37,440
+أغلبيتهم يشتكون من مرض الـ ulcers وهو مرض القرحة
+
+651
+00:47:37,440 --> 00:47:41,130
+في المعدة، قاموا بعمل دراسة على تلك الفئة، أعطوهم
+
+652
+00:47:41,130 --> 00:47:44,650
+ما يسمى بالـ lactobacillus acidophilus وهي بكتيريا
+
+653
+00:47:44,650 --> 00:47:49,430
+اللبن الرائب وبعد أن عدة شهور أو عدة أسابيع قاموا 
+
+654
+00:47:49,430 --> 00:47:53,510
+بعمل إحصائية مرة أخرى وجدوا أن هؤلاء الأشخاص المصابين
+
+655
+00:47:53,510 --> 00:47:57,370
+بمرض الـ migraine headaches قد شفوا منهم شفاءً تاماً
+
+656
+00:47:57,560 --> 00:48:01,580
+والقسم الآخر قد خفت وحدت هذا الألم بنسبة كبيرة جدا
+
+657
+00:48:01,580 --> 00:48:04,800
+وكل ذلك يعتمد على الحدة وعلى الـ environment، على
+
+658
+00:48:04,800 --> 00:48:07,600
+البيئة التي يعيشون بها، ما بين الاكتئاب والتوتر
+
+659
+00:48:07,600 --> 00:48:13,970
+وما إلى ذلك، هناك أشخاص مصابين بهذا المرض، وأكثر،
+
+660
+00:48:13,970 --> 00:48:20,530
+لكن من أصعب الشعور أن يكون شخص مقرب لك ويكون مصاب
+
+661
+00:48:20,530 --> 00:48:25,370
+به، لأ ولم تكن بيدك أي حيلة، لكن اليوم استطعنا أن
+
+662
+00:48:25,370 --> 00:48:28,610
+نجد هذه الحيلة، اللهم اشفي جميع مرضانا، نهدي هذا
+
+663
+00:48:28,610 --> 00:48:31,970
+العمل بشكل أخص إلى الطالب العزيز على قلوبنا جميع،
+
+664
+00:48:31,970 --> 00:48:36,300
+اللهم اشفي أحبتنا يا الله، وفي الختام وأجمل ختام
+
+665
+00:48:36,300 --> 00:48:39,980
+بمسك الختام، صلوا على الحبيب اللي عندنا، نكون معاكم
+
+666
+00:48:39,980 --> 00:48:43,320
+نرمي العشاء ونور السفر مع تقديم فائقة الشكر جزيلا
+
+667
+00:48:43,320 --> 00:48:48,660
+لرفيق الدكتور عبد الرؤوف، السلام عليكم، طبعا تقديم
+
+668
+00:48:48,660 --> 00:48:54,170
+الصغيرة و لابتوب وطيبات الزمان كان يقولوا قولي من
+
+669
+00:48:54,170 --> 00:48:58,970
+صاحبك أقولك من أنت، اليوم بيقولوا قولي من الـ normal
+
+670
+00:48:58,970 --> 00:49:02,250
+flora in your intestine أقولك من أنت حقيقة يعني
+
+671
+00:49:02,250 --> 00:49:08,370
+واضح أن الـ evidence تتوالى باستمرار أنه على أهمية
+
+672
+00:49:08,370 --> 00:49:10,630
+الـ normal flora وخاصة بالـ intestine التلاجة على
+
+673
+00:49:10,630 --> 00:49:17,570
+حفاظ على صحة جيدة، يوم أن قمنا بتدخل كبشر وعطينا
+
+674
+00:49:17,570 --> 00:49:25,470
+مضادات حيوية وشكل مكتف وغيرنا من نوع الغذاء وطبيعته
+
+675
+00:49:25,470 --> 00:49:30,950
+تغيرت .. تغيرت الأمراض اللي تنتج لدى البشر، ممكن أن
+
+676
+00:49:30,950 --> 00:49:33,610
+الـ antibiotic تعمل على قتل الـ normal flora كمان
+
+677
+00:49:33,610 --> 00:49:37,310
+يعني تاخد حبة من الـ antibiotic ممكن غير أنها تقضي
+
+678
+00:49:37,310 --> 00:49:40,830
+على البكتيريا وممكن تقضي على نفس الـ normal flora
+
+679
+00:49:40,830 --> 00:49:44,350
+وبالتالي ممكن تسبب المرض للـ intestine صح هذا .. هذا
+
+680
+00:49:44,350 --> 00:49:44,690
+ممكن 
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/NQosy7xELT8.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/NQosy7xELT8.srt
new file mode 100644
index 0000000000000000000000000000000000000000..f779021df1835c67cc8197c93bd337d5dfbfc9b6
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/NQosy7xELT8.srt
@@ -0,0 +1,2359 @@
+1
+00:00:20,790 --> 00:00:24,910
+بسم الله الرحمن الرحيم الموضوع اليوم ما زلنا فيه
+
+2
+00:00:24,910 --> 00:00:30,610
+أو إكمال لما يتعلق بالبعض genesis of بكتيريا ويمكن 
+
+3
+00:00:30,610 --> 00:00:33,650
+المحاضرة السابقة ركزنا فيها أكثر شيء على ال
+
+4
+00:00:33,650 --> 00:00:37,110
+exotoxins اليوم سيكون تركيزنا أكثر على ال
+
+5
+00:00:37,110 --> 00:00:40,050
+endotoxin وبعدين هنعمل مقارنة بين ال exotoxin و
+
+6
+00:00:40,050 --> 00:00:44,030
+ال endotoxin ونشوف أيش الفروقات الرئيسية ما بينهم 
+
+7
+00:00:44,030 --> 00:00:45,030
+بشكل ملخص
+
+8
+00:00:47,450 --> 00:00:51,630
+الـ Lipopolysaccharide شفناها بأكثر بأكثر من شكل
+
+9
+00:00:51,630 --> 00:00:56,670
+لكن في المحصلة هو مكون من ثلاثة أجزاء رئيسية التي 
+
+10
+00:00:56,670 --> 00:01:01,830
+هي Lipid-A وال core polysaccharide بالإضافة إلى
+
+11
+00:01:01,830 --> 00:01:09,250
+اللي هو ال O-side chain  عشان يصير release للإندو
+
+12
+00:01:09,250 --> 00:01:13,490
+توكسين لازم يصير فيه cell wall lysis عشان يصير فيه
+
+13
+00:01:13,490 --> 00:01:16,110
+cell wall lysis غالباً لازم يكون فيه death
+
+14
+00:01:21,420 --> 00:01:26,020
+لكن في المحصلة أن ال release تبعه يكون ناتج أو
+
+15
+00:01:26,020 --> 00:01:31,850
+ناجم عن اللي هو ال lysis تبع ال LPS المركب قلنا 
+
+16
+00:01:31,850 --> 00:01:35,130
+عنه formaldehyde and heat resistant وحاولنا نعمل 
+
+17
+00:01:35,130 --> 00:01:39,790
+علاقة في المصطلح أو في الجملة هذه بين المعلومة
+
+18
+00:01:39,790 --> 00:01:44,830
+اللي ذكرناها عن التوكسويد في الإكسدوكسين التوكسويد
+
+19
+00:01:44,830 --> 00:01:49,870
+أنتجناه عبر معالجة بالformaldehyde أو بالحرارة
+
+20
+00:01:49,870 --> 00:01:53,790
+للاكسدوكسين لكن هنا فشلنا أن نحصل على توكسويد
+
+21
+00:01:53,790 --> 00:01:56,430
+لأنها resistant and formaldehyde resistant
+
+22
+00:02:01,160 --> 00:02:04,880
+المطمئن أيضاً أن ممكن لو ضفنا adjuvant اللي هو
+
+23
+00:02:04,880 --> 00:02:09,140
+المادة ال carrier الحامل لو ركبنا على ال pre
+
+24
+00:02:09,140 --> 00:02:14,410
+-antigen ممكن نزيد من ال antigenicity تبعه في 
+
+25
+00:02:14,410 --> 00:02:16,890
+المحصلة النهائية اتفقنا أن ال life of
+
+26
+00:02:16,890 --> 00:02:20,430
+polysaccharide هو poor antigen بالمقارنة مع اللي هو
+
+27
+00:02:20,430 --> 00:02:24,550
+ال exotoxin اللي طبيعي تكوينه أصلاً protein وهذا
+
+28
+00:02:24,550 --> 00:02:28,270
+السبب الرئيسي في الفرق بين الاثنين ال protein is
+
+29
+00:02:28,270 --> 00:02:34,230
+more antigenic than اللي هو ال polysaccharide ال
+
+30
+00:02:34,230 --> 00:02:40,750
+endotoxin من أهم ال effects تبعته أنه بيعملنا
+
+31
+00:02:40,750 --> 00:02:46,840
+fever وبيسموه pyrogen طلع الرقم اللي بدأ أتكلمكوا
+
+32
+00:02:46,840 --> 00:02:51,800
+عنه الآن واحد microgram لكل kilogram كافٍ أنه 
+
+33
+00:02:51,800 --> 00:02:56,680
+يعملوا حرارة عند المريض واحد micro .. نتكلم عن
+
+34
+00:02:56,680 --> 00:02:59,520
+microgram .. micro .. نتكلمش عن milligram .. نتكلم
+
+35
+00:02:59,520 --> 00:03:06,780
+عن microgram طيب هذا الفبر .. الآن لو طلعنا على ال
+
+36
+00:03:06,780 --> 00:03:10,440
+effect الثاني اللي اسمه disseminated intravascular
+
+37
+00:03:10,440 --> 00:03:15,580
+coagulation أو باختصار DIC Disseminated
+
+38
+00:03:15,580 --> 00:03:19,600
+Intravascular Coagulation يعني جلطات منتشرة في
+
+39
+00:03:19,600 --> 00:03:26,990
+الجهاز الدوري للإنسان في كل مكان ليش بيعمل هيك هو؟
+
+40
+00:03:26,990 --> 00:03:29,570
+عنده Coagulase هو؟ فاكرين اللي Coagulase سابعة
+
+41
+00:03:29,570 --> 00:03:32,310
+السنة في الكوكاست بس هذا ال bacteria ماعندهاش
+
+42
+00:03:32,310 --> 00:03:36,130
+Coagulase عارفين ليش عندها؟ عندها ال endotoxin عنده
+
+43
+00:03:36,130 --> 00:03:40,050
+مقدرة أنه يعمل activation لل clotting cascade اللي
+
+44
+00:03:40,050 --> 00:03:45,850
+هي عملية التجلط في الدم المفروض أنه بشكل طبيعي
+
+45
+00:03:45,850 --> 00:03:50,810
+تتمّ في الجروح لكن وجود ال endotoxin بيعمل
+
+46
+00:03:50,810 --> 00:03:52,870
+activation اللي هتتمّ في الدم
+
+47
+00:03:57,530 --> 00:04:01,630
+concentration of one milligram per kilogram of
+
+48
+00:04:01,630 --> 00:04:08,110
+endotoxin is lethal at one
+
+49
+00:04:08,110 --> 00:04:11,290
+milligram per kilogram طبعاً هذا التركيز خدوا
+
+50
+00:04:11,290 --> 00:04:16,890
+بالكم بالمقارنة مع ال exotoxin تركيز عالي جداً ال
+
+51
+00:04:16,890 --> 00:04:21,570
+exotoxin قد يكون التركيز أقل من ذلك ويكون قاتل و
+
+52
+00:04:21,570 --> 00:04:22,650
+أنا ذكرت لكم مثال
+
+53
+00:04:30,540 --> 00:04:40,010
+البوتلونيوم كافٍ لقتل عشرين مليون فرقم مجرد تذوق،
+
+54
+00:04:40,010 --> 00:04:43,770
+وعارفين كلمة تذوق؟ قلنا مش أكل، مش تناول، تذوق
+
+55
+00:04:43,770 --> 00:04:48,010
+طعام يحتوي على البوتراناتوكسين كافٍ لقتل الإنسان،
+
+56
+00:04:48,010 --> 00:04:52,510
+إذن احنا okay واحد مللي جرام تركيز قليل، لكن ال X
+
+57
+00:04:52,510 --> 00:04:57,830
+-Toxin أقل منه كمان، كمان ال X-Toxin أقل طيب، من
+
+58
+00:04:57,830 --> 00:04:59,930
+ال effects تبعته أنه بيعمل non-specific
+
+59
+00:04:59,930 --> 00:05:02,750
+inflammation عارفين ليش يعني non-specific
+
+60
+00:05:02,750 --> 00:05:06,210
+inflammation؟ يعني كل ال immune system بين الشغل
+
+61
+00:05:06,210 --> 00:05:10,310
+في حالة cytokine طبعاً نجد عالمين عن cytokine
+
+62
+00:05:10,310 --> 00:05:15,190
+release اللي هو ال chemical messengers كمان بيعمل
+
+63
+00:05:15,190 --> 00:05:18,410
+حاسمة complement activation وياريت نفهم هذا
+
+64
+00:05:18,410 --> 00:05:21,170
+الجزء للبعض اللي ما درسش مناعة ما بيعرفش إيش يعني
+
+65
+00:05:21,170 --> 00:05:26,170
+complement فبنوضح شو يعني complement ال complement 
+
+66
+00:05:26,170 --> 00:05:30,830
+عبارة عن بروتينات موجودة في الجسم بشكل inactive
+
+67
+00:05:30,830 --> 00:05:34,970
+إيش يعني inactive؟ يعني مثل هذا القلم بالضبط طلع
+
+68
+00:05:34,970 --> 00:05:40,480
+عليه القلم هذا ما حد بينكر أنه قلم لكن لو حاولنا
+
+69
+00:05:40,480 --> 00:05:45,960
+نكتب فيه بالطريقة دي مش هيكتب لأنه inactive سبب
+
+70
+00:05:45,960 --> 00:05:49,340
+أنه inactive أنه فيه غطاء لو فكينا الغطاء بصير إيش هو
+
+71
+00:05:49,340 --> 00:05:52,180
+أكثر طبعاً من رحمة الله سبحانه وتعالى فينا من الجسم
+
+72
+00:05:52,180 --> 00:05:59,980
+أنه inactive لأنه لا تنشط إلا ب specific events
+
+73
+00:05:59,980 --> 00:06:04,240
+غالباً لقتل البكتيريا لقتل البكتيريا تخيل
+
+74
+00:06:04,240 --> 00:06:10,340
+اندوكسين يش بيعمل بنشطها طب الاندوكسين بده يدي حاله؟
+
+75
+00:06:10,340 --> 00:06:15,500
+لا بنشطها في مواضع مش مناسبة للدسن فبالتالي بتقتل 
+
+76
+00:06:15,500 --> 00:06:18,500
+خلايا مين؟ خلايا الدسن فبصير destruction لخلايانا
+
+77
+00:06:18,500 --> 00:06:21,360
+احنا بال complement activation طب شيه ال
+
+78
+00:06:21,360 --> 00:06:23,500
+complement احنا ما عرفناه عشان صحيح ال complement
+
+79
+00:06:23,500 --> 00:06:27,780
+هي عبارة عن group of proteins غالباً بيرمزولها ب C
+
+80
+00:06:27,780 --> 00:06:34,940
+فهي مرتبة من C1 ل C9 يعني C1, C2, C3 وهكذا أنا مش
+
+81
+00:06:34,940 --> 00:06:39,670
+وصفتها زرجلال ما بيصير ال activation غالباً بتنقسم
+
+82
+00:06:39,670 --> 00:06:44,330
+الجسمين fragment A و fragment V برضه إذا بيسموه
+
+83
+00:06:44,330 --> 00:06:49,670
+C5A C5V مثلاً على ال complement خمسة A وكل واحدة
+
+84
+00:06:49,670 --> 00:06:52,990
+منهم بيكون لها function طبعاً مش مجلة مش مجلة
+
+85
+00:06:52,990 --> 00:06:56,510
+لأن نتكلم فيهم الآن هدرسوهم إن شاء الله في المناعة
+
+86
+00:06:56,510 --> 00:07:01,890
+بشكل دقيق وهتعرفوا كيف بيشتغلوا وكيف بيتنشطوا
+
+87
+00:07:01,890 --> 00:07:13,510
+أصلابيسل ميتوجين بيحفظ الخلايا على الانقسام يعني
+
+88
+00:07:13,510 --> 00:07:20,870
+وجوده مدفوع للانقسام الكل 
+
+89
+00:07:20,870 --> 00:07:26,710
+كلونال بيسل activators أول حاجة أرفها مش يعني
+
+90
+00:07:26,710 --> 00:07:33,980
+كلونال كلون معناها سلالة كلون معناها سلالة أيش
+
+91
+00:07:33,980 --> 00:07:38,280
+سلالة بتكون؟ ناشئة من إيش؟ من one single cell،
+
+92
+00:07:38,280 --> 00:07:43,620
+سلالة، نوع معين، فلما نقول monoclonal بنقول سلالة
+
+93
+00:07:43,620 --> 00:07:49,810
+واحدة، لما نقول polyclonal عدّة سلالات المفروض أن في
+
+94
+00:07:49,810 --> 00:07:54,230
+جهة الملعقة بتقول لكم كل حاجة اسمها specific فلما
+
+95
+00:07:54,230 --> 00:07:57,950
+احنا بننشط بننشط سلالة معينة من ال B-cells
+
+96
+00:07:57,950 --> 00:08:02,030
+لإنتاجها على سبيل المثال ال antibodies تخيلوا أن
+
+97
+00:08:02,030 --> 00:08:07,510
+الاندوكسين بيعمل activation لمجموعة كبيرة من ال B
+
+98
+00:08:07,510 --> 00:08:10,850
+-cells كل واحدة منها بتنتج سلالة لو افترضنا أنهم
+
+99
+00:08:10,850 --> 00:08:15,830
+كلهم معينين لقيام بوظيفة واحدة هل يستطيعوا الآلاف
+
+100
+00:08:15,830 --> 00:08:20,410
+اللي طلعت تقوم بهذه الوظيفة؟ لا يستطيعوا لأن
+
+101
+00:08:20,410 --> 00:08:24,790
+المخصصة لنفوت كونها one group فلما سينا دي
+
+102
+00:08:24,790 --> 00:08:29,110
+polyclonal B cell activator و B cell mitogen يعني
+
+103
+00:08:29,110 --> 00:08:32,330
+ثلاثة مختلفة و complement activation و cytokine
+
+104
+00:08:32,330 --> 00:08:37,410
+release و non specific inflammation عشان هيك بنقول
+
+105
+00:08:37,410 --> 00:08:42,030
+احنا وبنذكركم لما درسنا ال X-Toxin كنا نتكلم عن
+
+106
+00:08:42,030 --> 00:08:46,050
+one step كنا نقول أن it inhibits the release of
+
+107
+00:08:46,050 --> 00:08:50,410
+acetylcholine it inhibits the release of glycine
+
+108
+00:08:50,410 --> 00:08:54,420
+اللي بيمنع ال acetylcholine it can degrade
+
+109
+00:08:54,420 --> 00:08:58,580
+ribosomal RNA therefore it will prevent protein
+
+110
+00:08:58,580 --> 00:09:02,260
+synthesis it will inactivate elongation factor 2
+
+111
+00:09:02,260 --> 00:09:05,220
+to prevent protein synthesis كل هذا بقى عن one
+
+112
+00:09:05,220 --> 00:09:09,900
+single step very specific step بس في ال endotoxin
+
+113
+00:09:10,580 --> 00:09:13,280
+الجانبي بيتكلم عن non specific inflammation،
+
+114
+00:09:13,280 --> 00:09:16,160
+بيتكلم عن fever، عن disseminated intravascular
+
+115
+00:09:16,160 --> 00:09:19,920
+coagulation، عن complement activation، عن B cell
+
+116
+00:09:19,920 --> 00:09:23,140
+mitogenes، عن polyclonal B cell activation، يفجر
+
+117
+00:09:23,140 --> 00:09:28,980
+في عندي عاصفة من الأحداث تنتج عن حضرة من ال
+
+118
+00:09:28,980 --> 00:09:36,980
+endotoxin هذا الشكل يعني بيعطينا التداخلات المتنوعة
+
+119
+00:09:36,980 --> 00:09:40,240
+للاندوكسين مش هركز عليه كثير تقريباً كله ذكر في
+
+120
+00:09:40,240 --> 00:09:44,200
+اللوحات اللي هو السابقة لكن اللوحة اللي أمامنا هذه
+
+121
+00:09:44,200 --> 00:09:48,100
+مهمة كثير لأنها بتلخص الفروقات بين الاندوكسين
+
+122
+00:09:48,100 --> 00:09:51,620
+والإفستوكسين وانت تبقى نركز على اللوحة بشكل مستمر
+
+123
+00:09:51,620 --> 00:09:56,400
+حتى ننهي منها إن شاء الله لاحظوا أول شيء ال chemical
+
+124
+00:09:56,400 --> 00:10:01,710
+nature ال .. ال .. ال .. ال exoxin مكون من ال
+
+125
+00:10:01,710 --> 00:10:04,950
+protein بينما ال endoxin مكون من إيش؟ من ال
+
+126
+00:10:04,950 --> 00:10:07,390
+lipopolysaccharide، رحظة الـ molecular weight تبع
+
+127
+00:10:07,390 --> 00:10:12,530
+التنين، أقل، أقل من الأقل؟ الـ lipopolysaccharide 
+
+128
+00:10:12,530 --> 00:10:16,950
+أقل من الـ protein، وعلى فكرة أحد سمات التمييز في
+
+129
+00:10:16,950 --> 00:10:19,250
+الـ antigenات، يعني لو ننصن في الـ antigenات من
+
+130
+00:10:19,250 --> 00:10:23,770
+strong لبور، تمام؟ الـ chemical structure له دور كبير
+
+131
+00:10:23,770 --> 00:10:28,050
+في التصنيف رقم اتنين الـ molecular weight فاكر لما
+
+132
+00:10:28,050 --> 00:10:31,890
+قلت إنه الـ free molecule لو ركّبناها على carrier
+
+133
+00:10:31,890 --> 00:10:35,830
+بزيد من الـ antigenicity تبقى الـ size مهم، صح؟ الـ
+
+134
+00:10:35,830 --> 00:10:41,250
+size مهم. relationship to cell علاقة الـ toxin 
+
+135
+00:10:41,250 --> 00:10:44,450
+بالخلية الـ endotoxin هو integral part
+
+136
+00:10:56,680 --> 00:11:01,680
+الخلية ينتج بالخلية من الداخل وبعدين يخرج الخلية
+
+137
+00:11:01,680 --> 00:11:09,440
+خارج الخلية while alive و الخلية لسه حية denatured 
+
+138
+00:11:09,440 --> 00:11:12,940
+by boiling هل بيصيله denaturation طبعًا لأنه
+
+139
+00:11:12,940 --> 00:11:17,300
+protein هناك الـ .. الـ .. الـ exotoxin بصيرله
+
+140
+00:11:17,300 --> 00:11:20,300
+denaturation بينما الأولاني و ذكرناها في الأوحى
+
+141
+00:11:20,300 --> 00:11:23,840
+السابقة إنه لا بصيرله denaturation لا بالـ formalin
+
+142
+00:11:23,840 --> 00:11:29,780
+ولا بالهرارة ولأنه formalin and heat stay الـ
+
+143
+00:11:29,780 --> 00:11:34,140
+antigenic yes و yes بس ممكن تضيفه كلمتين بعد الـ
+
+144
+00:11:34,140 --> 00:11:39,220
+yes الأولانية poor وبعد الـ yes التانية strong احنا
+
+145
+00:11:39,220 --> 00:11:41,160
+بنميز بين التانية، لإن التانية عندهم antigenicity
+
+146
+00:11:41,160 --> 00:11:45,000
+الصحية، لكن واحدة ضعيفة وواحدة قوية، مين الضعيفة؟
+
+147
+00:11:45,000 --> 00:11:50,560
+الـ .. الـ endotoxin الـ potency، الفعالية، مين أكثر
+
+148
+00:11:50,560 --> 00:11:57,380
+فعالية؟ الـ exotoxin والـ endotoxin اتفقنا كـ .. لا
+
+149
+00:11:57,380 --> 00:12:01,180
+.. لا .. انتوا الآن .. هـ .. هـ .. بدنا نشيل الـ
+
+150
+00:12:01,180 --> 00:12:05,300
+confusion اللي حصل هذا الـ confusion اللي حصل إنه
+
+151
+00:12:05,300 --> 00:12:09,570
+الفعالية غير عن الـ mechanism of action الـ
+
+152
+00:12:09,570 --> 00:12:13,210
+mechanism of action في الـ exotoxin كانت specific،
+
+153
+00:12:13,210 --> 00:12:16,870
+في الـ endotoxin كانت multiple، صح؟ هنا بتتكلم عن
+
+154
+00:12:16,870 --> 00:12:20,490
+concentration capable of doing the job، يعني
+
+155
+00:12:20,490 --> 00:12:25,430
+التركيز اللي بيقوم بالمهمة، مين أقل تركيز، الـ
+
+156
+00:12:25,430 --> 00:12:29,450
+exotoxin تركيزه أقل، فبالتالي فعليته يكون أعلى،
+
+157
+00:12:29,450 --> 00:12:33,730
+هاي المفهوم اللي احنا بنقصده فيه في الـ potency، الـ
+
+158
+00:12:33,730 --> 00:12:37,970
+specificity الآن low degree أنا قلتلكوا أنا بعمل
+
+159
+00:12:37,970 --> 00:12:40,710
+non-specific inflammation وبيعمل fever وبيعمل
+
+160
+00:12:40,710 --> 00:12:44,110
+complement activation و cascade للـ .. للـ .. للـ
+
+161
+00:12:44,110 --> 00:12:47,170
+للـ clotting factor activation وبالتالي هو بالفعل
+
+162
+00:12:47,170 --> 00:12:50,130
+non-specific عارفين بيذكروننا بإيش؟ كويسة الـ
+
+163
+00:12:50,130 --> 00:12:55,230
+analogy لما اتكلمنا عن الـ .. الـ antibiotics و
+
+164
+00:12:55,230 --> 00:12:59,290
+اتكلمنا عن الـ disinfectants لما اتكلمنا عن الـ
+
+165
+00:12:59,290 --> 00:13:03,190
+disinfectant جينا إن الـ disinfectant بيقتل عن
+
+166
+00:13:03,190 --> 00:13:05,870
+طريق الـ protein denaturation وبيقتل عن طريق الـ
+
+167
+00:13:05,870 --> 00:13:09,210
+DNA نفس الـ disinfectant الواحد بينما الـ antibiotic
+
+168
+00:13:09,210 --> 00:13:15,510
+بكون له one specific step فبالتالي .. فبالتالي كان
+
+169
+00:13:15,510 --> 00:13:17,910
+سهل على البكتيريا اللي بتذكر وبيبقى تعمل
+
+170
+00:13:17,910 --> 00:13:23,490
+resistance لمن؟ للـ .. للـ antibiotic لكان صعب تعمل
+
+171
+00:13:23,490 --> 00:13:26,210
+resistance لمن؟ للـ .. اللي هو الـ disinfectant 
+
+172
+00:13:30,990 --> 00:13:34,630
+Enzymatic activity هل يمتلك الـ toxin Enzymatic
+
+173
+00:13:34,630 --> 00:13:38,450
+activity بالنسبة للـ endotoxin إيش الجواب؟ No بينما
+
+174
+00:13:38,450 --> 00:13:41,990
+الـ exotoxin ولو رجعتوا اللوحات من المحاضرة السابقة
+
+175
+00:13:41,990 --> 00:13:47,730
+ختمناها بـ Enzyme سمينا هم exotoxin فالـ exotoxin هم
+
+176
+00:13:47,730 --> 00:13:49,030
+بالفعل هم Enzymatic
+
+177
+00:13:51,870 --> 00:13:55,470
+الـ pyrogenicity والـ pyrogenicity قدرة الميكروب
+
+178
+00:13:55,470 --> 00:13:58,730
+على رفع درجة حرارة الجسم يعني حرارة شوفوا نجروا شوية
+
+179
+00:13:58,730 --> 00:14:05,250
+إنّه الـ endotoxin بيعمل بينما الـ exotoxin مش كان ..
+
+180
+00:14:05,250 --> 00:14:08,930
+مابيعملش غالبًا لكن هنا .. هنا حاطين احنا جملة
+
+181
+00:14:08,930 --> 00:14:13,210
+occasionally لإنه في بعض الـ exotoxin قد ترفع درجة
+
+182
+00:14:13,210 --> 00:14:14,130
+اللي هو الحرارة
+
+183
+00:14:16,730 --> 00:14:20,030
+طيب هذا التركيب العظيم في الـ gram negative بكتيريا
+
+184
+00:14:20,030 --> 00:14:24,670
+المسمى بالـ lipopolysaccharide أو الـ endotoxin هل
+
+185
+00:14:24,670 --> 00:14:27,790
+يولد لهم مقابل counterpart في الـ gram positive هو
+
+186
+00:14:27,790 --> 00:14:31,950
+خاص في الـ gram negative صح؟ لأن هل لو counterpart
+
+187
+00:14:31,950 --> 00:14:36,470
+في إجابة بتقول أنا أقصده ولا نعم؟ بتقول yes اللي
+
+188
+00:14:36,470 --> 00:14:39,710
+بيبتدوا شرايكم اللي موجود في الـ gram positive may
+
+189
+00:14:39,710 --> 00:14:43,150
+play a role similar to that of endotoxin of gram
+
+190
+00:14:43,150 --> 00:14:47,270
+negative bacteria يعني .. يعني الـ .. الـ gram
+
+191
+00:14:47,270 --> 00:14:50,390
+positive الأصل إنه مالهاش ليه و lipopolysaccharide طيب
+
+192
+00:14:50,390 --> 00:14:52,870
+ليش بتطلع حرارة؟ ليش بتعمل intravascular
+
+193
+00:14:52,870 --> 00:14:55,990
+coagulation؟ ليش بتعمل .. لإن .. لإن الـ
+
+194
+00:14:55,990 --> 00:14:59,590
+peptidoglycan تبعها بيشتغل بنفس الطريقة اللي
+
+195
+00:14:59,590 --> 00:15:02,950
+بيشتغل فيها مين إن هو الـ lipopolysaccharide تبع الـ
+
+196
+00:15:02,950 --> 00:15:06,530
+gram negative؟ طيب enzymes
+
+197
+00:15:08,260 --> 00:15:11,080
+أظن نزجنا احنا من المحاضرة الماضية وهنقول
+
+198
+00:15:11,080 --> 00:15:16,320
+hyaluronidase و collagenase و elastase إنزيمات كلها
+
+199
+00:15:16,320 --> 00:15:20,460
+بتكسر الانسجة ليها tissue degrading enzymes لكن
+
+200
+00:15:20,460 --> 00:15:24,620
+اللي جديد في اللوحة دي شيء رائع جدًا يجب أن ننتبه
+
+201
+00:15:24,620 --> 00:15:28,980
+له بشكل كثير أنا أفهم إن البعض منكم ما درسش مناعة
+
+202
+00:15:28,980 --> 00:15:32,300
+ولا عنده أي خلفية عن الـ immunoglobulins الـ
+
+203
+00:15:32,300 --> 00:15:36,630
+antibodies الأجسام المضادة وبالتالي كانوا يتحملون
+
+204
+00:15:36,630 --> 00:15:39,250
+اللي .. اللي عندهم خلفية في هذا الموضوع يتحملونها
+
+205
+00:15:39,250 --> 00:15:44,570
+الجسم بينتج خمس أنواع من الأجسام المضادة بلاخصهم
+
+206
+00:15:44,570 --> 00:15:48,950
+في كلمة ماجد Ig .. Ig immunoglobulin الـ
+
+207
+00:15:48,950 --> 00:15:52,410
+immunoglobulin هو وصف آخر لمين؟ للـ antibody يعني
+
+208
+00:15:52,410 --> 00:15:57,550
+الآن اللي قدامي على اللوحة هنا IgA immunoglobulin
+
+209
+00:15:57,550 --> 00:16:04,400
+A في من .. من بدل الـ A كمان في أربع في IgG و IgM و
+
+210
+00:16:04,400 --> 00:16:10,500
+IgE و IgD واحنا قلنا الـ A الخامس وسميناهم ماجد
+
+211
+00:16:10,500 --> 00:16:16,100
+حلوين الـ antibodies أنا لو بعرف إنكم مش دارسينهم
+
+212
+00:16:16,100 --> 00:16:18,460
+كان عملتلكوا صورة برضه للموضوع لأنه مهم بالنسبة
+
+213
+00:16:18,460 --> 00:16:22,400
+لنا كثير مهم الموضوع اللي بهمني في اللوحة هذه إن
+
+214
+00:16:22,400 --> 00:16:27,580
+كنتوا عارفين إن الـ IgA هو ليس أهمهم على الإطلاق لكن
+
+215
+00:16:27,580 --> 00:16:34,840
+له ميزة مهمة جدًا إنه soluble مش إنه soluble ذائب و
+
+216
+00:16:34,840 --> 00:16:37,740
+مدام ذائب فبنلاقيه في اللعاب بنلاقيه على mucous
+
+217
+00:16:37,740 --> 00:16:43,120
+membrane فهو الدفاع أو خط الدفاع الأول لـ specific
+
+218
+00:16:43,120 --> 00:16:49,800
+الأول اللي بيكون على الـ membranes طيب الآن افترض
+
+219
+00:16:49,800 --> 00:16:54,260
+إنّه في عندي immunoglobulin A تبع Streptococcus
+
+220
+00:16:54,260 --> 00:16:57,420
+pneumonia، والـ Streptococcus pneumonia دخلت الـ
+
+221
+00:16:57,420 --> 00:17:01,700
+lungs، إيش بيعمل فيها المفروض؟ بيمسك فيها و
+
+222
+00:17:01,700 --> 00:17:07,160
+بدمرها، فحضرتها بتجيه وجاهزة معاها سلاح يكسرها
+
+223
+00:17:07,160 --> 00:17:12,880
+السلاح يبقى الـ IgA هو السلاح تبعنا احنا، سلاح
+
+224
+00:17:12,880 --> 00:17:17,720
+دفاعي، هي جايبة معاها سلاح هجومي ضد الدفاعي، اللي هو
+
+225
+00:17:17,720 --> 00:17:23,080
+IgA Protease، انزين بيكسر الـ immunoglobulin، تمام؟
+
+226
+00:17:23,080 --> 00:17:26,940
+لن تستطيع إن هتعمل infection في ذاك المكان أو
+
+227
+00:17:26,940 --> 00:17:29,300
+attachment في ذاك المكان إلا بهذا السلاح، شوفوا
+
+228
+00:17:29,300 --> 00:17:33,000
+مجموعة كبيرة من الميكروبات الخطيرة جدًا، اللي مرض
+
+229
+00:17:33,000 --> 00:17:37,890
+معاكوا أكتر من مرة، زي Hemophilus influenza ستربتوكوكاس
+
+230
+00:17:37,890 --> 00:17:43,530
+نيمونيا نيسيريا جونريا ونيسيريا مينينجتس لن
+
+231
+00:17:43,530 --> 00:17:46,630
+يستطيعوا إنهم يعملوا infection إلا بهذا السلاح
+
+232
+00:17:46,630 --> 00:17:51,110
+اللي هو الـ IgA Protease تمام؟ ثالثة معلومتان في
+
+233
+00:17:51,110 --> 00:17:56,670
+اللوحة هذه إن فيها إنّه soluble antibodies that
+
+234
+00:17:56,670 --> 00:17:59,310
+will protect mucous membranes والـ mucous membrane
+
+235
+00:17:59,310 --> 00:18:03,770
+ليش بدنا protection خاصة فيه؟ بنفترض المتاحة من
+
+236
+00:18:03,770 --> 00:18:08,270
+تبع العين أو الأنف أو الفم أو الورثرة أو الـ vagina
+
+237
+00:18:08,270 --> 00:18:12,430
+من جوا مافيش فيهم skin واحنا بنتنا نتنا انت نتمتع
+
+238
+00:18:12,430 --> 00:18:17,710
+بالجلد برة كحماية فعشان نحمي هذه المناطق تمام بدنا
+
+239
+00:18:17,710 --> 00:18:23,570
+اللي هو السلاح اللي اسمه IgA لكن البكتيريا اجتوا
+
+240
+00:18:23,570 --> 00:18:29,670
+معاها counter weapon يعني سلاح مضاد لهذا السلاح
+
+241
+00:18:31,930 --> 00:18:37,290
+Anti-phagocytic factors عوامل مضادة للـ .. للـ .. للـ
+
+242
+00:18:37,290 --> 00:18:40,970
+phagocytosis برضه كمان احنا الآن أخذوا بالكم نتكلم
+
+243
+00:18:40,970 --> 00:18:47,510
+على احنا لدينا جهاز مناعة انتج IgA جبنا له سلاح كسره
+
+244
+00:18:47,510 --> 00:18:50,950
+أنا عندي في جهاز المناعة phagocytes و macrophages هي
+
+245
+00:18:50,950 --> 00:18:55,550
+الآن نتكلم على كيفية مكافحة اللي هو الـ phagocytes
+
+246
+00:18:55,550 --> 00:19:00,460
+والـ .. الـ phagocytosis والـ macrophages في خدعة
+
+247
+00:19:00,460 --> 00:19:10,580
+قديمة كان زمان واحد ما يعرف يلبس على كتفه مثلًا جلد
+
+248
+00:19:10,580 --> 00:19:16,300
+خروف ويروح يعني يدخل بين الخرفان ويتخبى لسبب ما
+
+249
+00:19:16,300 --> 00:19:20,650
+يتخبى فـ he is unrecognized ليش unrecognized؟ لأنه
+
+250
+00:19:20,650 --> 00:19:24,730
+استخدم material من المجموعة فتخيلوا إنه في بكتيريا 
+
+251
+00:19:24,730 --> 00:19:28,530
+عندها المقدرة أنها تأخذ من الإنزيمات تبعتنا و تحط على
+
+252
+00:19:28,530 --> 00:19:32,870
+حالها أو من الـ chemicals اللي تبعتنا و تحط الدهن
+
+253
+00:19:32,870 --> 00:19:36,870
+حالها على الأسطح تبعها و بتصير لزق منها فهيك
+
+254
+00:19:36,870 --> 00:19:42,790
+تبقى لمن؟ بالظبط زي أنا أعطيكم مثال بسيط لما أخذنا
+
+255
+00:19:42,790 --> 00:19:46,110
+الجلطات من الـ coagulase في الـ staphylococcus aureus 
+
+256
+00:19:46,110 --> 00:19:50,710
+أول مناسبة هحط لكم بقى فيديو على الـ Facebook اسمه .. اسمه
+
+257
+00:19:50,710 --> 00:19:55,770
+الفيديو Virulence Factor لـ Staphylococcus aureus
+
+258
+00:19:55,770 --> 00:19:58,570
+و أنا أعتقد أنه لازم تشوفوه كلكم لأنه بيوضّح لكم 
+
+259
+00:19:58,570 --> 00:20:02,410
+الـ .. الـ Virulence Factor mechanism of action بي ..
+
+260
+00:20:02,410 --> 00:20:04,850
+بي .. بي .. في كل خطوة من خطوات الـ infection من
+
+261
+00:20:04,850 --> 00:20:09,650
+دخول البكتيريا إلى الـ dissemination تبعت على ال ..
+
+262
+00:20:09,650 --> 00:20:12,090
+الـ .. الموقع تبعتنا، المجموعة دي اللي هي Essential
+
+263
+00:20:12,090 --> 00:20:17,170
+Medical Microbiology F أمام؟ اللي هي الـ female طيب
+
+264
+00:20:17,170 --> 00:20:20,890
+النوع الثاني few bacteria produces soluble factors
+
+265
+00:20:20,890 --> 00:20:24,830
+or toxins that inhibit chemotaxis by leukocytes
+
+266
+00:20:24,830 --> 00:20:31,390
+and thus evade phagocytosis الآن الـ cytokines
+
+267
+00:20:31,390 --> 00:20:37,750
+وظيفتها الرئيسية chemical messengers خلية أصيبت
+
+268
+00:20:37,750 --> 00:20:40,890
+بستمر release لـ cytokines الـ cytokines بتقول لـ الـ 
+
+269
+00:20:40,890 --> 00:20:43,790
+inflammatory cells ومن ضمنها الـ phagocytes تعالوا
+
+270
+00:20:43,790 --> 00:20:47,630
+إلى هذا المكان هناك بكتيريا موجودة في المكان
+
+271
+00:20:47,630 --> 00:20:54,470
+البكتيريا ما عندهاش سلاح لمكافحة الفاجوسايت أو
+
+272
+00:20:54,470 --> 00:21:00,090
+للبقاء حي جوا الفاجوسايت إيش بتعمل؟ بتنتج chemical
+
+273
+00:21:00,090 --> 00:21:05,630
+هذا الـ chemical بيفهم الـ phagocyte إن هذا false
+
+274
+00:21:05,630 --> 00:21:11,790
+alarm يعني إنذار خاطر إنذار خاطر صعب حالة بترجع
+
+275
+00:21:11,790 --> 00:21:17,610
+الـ phagocyte لغة Chemicals بنبعت لبعض نخترخ جوّالات
+
+276
+00:21:17,610 --> 00:21:20,630
+بعض و بنبعت لبعض مزيجيز مضادة واحد بيقولي تعالي و
+
+277
+00:21:20,630 --> 00:21:26,210
+أنا بقوله ياه ما تيجيش فالبكتيريا بتنتج Chemicals
+
+278
+00:21:26,210 --> 00:21:29,630
+to prevent the chemotactic activity of اللي هو
+
+279
+00:21:29,630 --> 00:21:35,750
+الخلايا تبعها لكن تعالوا نطلع على اللوحة هذه اللي
+
+280
+00:21:35,750 --> 00:21:40,830
+فيها معلومات ضخمة و كثيرة عن عوامل محددة تستخدم ك
+
+281
+00:21:40,830 --> 00:21:42,190
+antiphagocytic
+
+282
+00:21:44,000 --> 00:21:47,180
+درست واحدة منها مهمة جدا اللي هي الـ capsule، أعتقد
+
+283
+00:21:47,180 --> 00:21:51,500
+أنه كل واحد عرف إن الـ capsule أحد مهمها كان لها
+
+284
+00:21:51,500 --> 00:21:55,840
+وظيفتها لأي سيطرة، مظبوط، أشوش هما antiphagocytosis
+
+285
+00:21:55,840 --> 00:22:00,640
+و الثانية attachment، و يوم ما قلنا عكس بعض
+
+286
+00:22:00,640 --> 00:22:05,720
+التنتين صح؟ طيب الـ polysaccharide capsule of
+
+287
+00:22:05,720 --> 00:22:10,580
+streptococcus pneumoniae Hemophilus تقول أسمها بدأت
+
+288
+00:22:10,580 --> 00:22:13,380
+تتكرر معانا و صارت familiar اسمها Hemophilus
+
+289
+00:22:13,380 --> 00:22:19,080
+influenza Treponema pallidum Bacillus anthracis اه
+
+290
+00:22:19,080 --> 00:22:22,560
+في غلطة كبيرة هنصحّيها حطوا خط تحت Bacillus
+
+291
+00:22:22,560 --> 00:22:27,300
+anthracis و اكتبوا عليها exception مش 
+
+292
+00:22:27,300 --> 00:22:31,160
+polysaccharide هي مش polysaccharide هي polypeptide
+
+293
+00:22:31,160 --> 00:22:32,400
+polypeptide
+
+294
+00:22:36,750 --> 00:22:39,170
+and Klebsiella pneumoniae بعرف في المعمل اللي
+
+295
+00:22:39,170 --> 00:22:44,510
+بتعمله capsule stain in the ink شفته طبعا أكيد
+
+296
+00:22:44,510 --> 00:22:46,450
+البكتيريا اللي جابولكم إياها جابولكم اللي هو الـ
+
+297
+00:22:46,450 --> 00:22:49,810
+Klebsiella جابولكم الـ Klebsiella وشفته مصطلعات الـ
+
+298
+00:22:49,810 --> 00:22:51,210
+Klebsiella الـ large
+
+299
+00:22:53,540 --> 00:22:57,560
+Large و Mucoid عارفين إيش بيسموا الـ .. الـ .. الـ ..
+
+300
+00:22:57,560 --> 00:23:01,980
+الـ polysaccharide المكون للكبسول بعض الناس بيسموه
+
+301
+00:23:01,980 --> 00:23:05,760
+Mycopolysaccharide ليش؟ لأنه الـ .. الـ large
+
+302
+00:23:05,760 --> 00:23:10,200
+quantity منه Mucoid وكلمة Mucoid لزج صح؟ و
+
+303
+00:23:10,200 --> 00:23:15,480
+اللزوج عامل بيساعد بإنه على عدم قدرة التصاق الفاجو
+
+304
+00:23:15,480 --> 00:23:19,380
+ساعد بيه البكر المهم إن الجملة هذه اللي مارين
+
+305
+00:23:19,380 --> 00:23:23,260
+عليها المرسلة محتوية على معلومات خطيرة كثيرة من
+
+306
+00:23:23,260 --> 00:23:27,520
+ضمنها أسماء لمجموعة من الميكروبات كلها بتحمل على
+
+307
+00:23:27,520 --> 00:23:30,560
+ظهرها اللي هي الـ E-capsule فبنكون عارفين برضه إنّها
+
+308
+00:23:30,560 --> 00:23:36,800
+Decapsulated microorganism في تراكيب خاصة protein
+
+309
+00:23:36,800 --> 00:23:40,860
+M أو M-protein و الـ fimbriae في الـ Streptococcus
+
+310
+00:23:40,860 --> 00:23:43,560
+pyogenes اللي بتنتمي إلى مجموعة بيسميها group A
+
+311
+00:23:43,560 --> 00:23:46,200
+streptococci هدول بيساعدوها على الهروب برضه من
+
+312
+00:23:46,200 --> 00:23:51,740
+الفاجوسايت Pseudomonas aeruginosa رقم تلاتة
+
+313
+00:23:51,740 --> 00:23:56,380
+Pseudomonas aeruginosa بتعمل biofilm أعتقد إن أنا
+
+314
+00:23:56,380 --> 00:24:00,100
+مرة يعني .. بس أنا حابب إن أؤكد على إن الـ biofilm
+
+315
+00:24:00,100 --> 00:24:06,400
+هو عبارة عن الـ aggregation لـ microorganisms و
+
+316
+00:24:06,400 --> 00:24:10,680
+أؤكد على حقيقة إن تصرف البكتيريا كـ biofilm بيختلف
+
+317
+00:24:10,680 --> 00:24:18,350
+عن تصرفها كـ single microorganism إيش يعني بيختلف؟ لو
+
+318
+00:24:18,350 --> 00:24:23,390
+أنا عملت اختبار شفوي لحضاراتكم وكل طالب دخل عليّ
+
+319
+00:24:23,390 --> 00:24:28,590
+المكتب وسألتها سؤال أكيد فرصتها فيه لإن تجاوب صح
+
+320
+00:24:28,590 --> 00:24:34,270
+بتكون أقل من فرصة لو هي اتشاورت مع زميلة لها ولو
+
+321
+00:24:34,270 --> 00:24:38,070
+اتشاورت مع كل الفصل بتزيد فرص إنك تحصلوا إجابة إيه
+
+322
+00:24:38,070 --> 00:24:40,730
+طبعا أنا ما أشجعش الأسلوب هذا في الامتحانات لو
+
+323
+00:24:40,730 --> 00:24:44,760
+اتشاور مع بعض لكن في الآخر هذا الواقع اللي بيصير لو
+
+324
+00:24:44,760 --> 00:24:48,940
+احنا بدنا نطلع برأي معين في اجتماع أكيد الرأي
+
+325
+00:24:48,940 --> 00:24:53,220
+بيكون أفضل أما يكون أكثر البكتيريا بلات لما بتكون
+
+326
+00:24:53,220 --> 00:24:56,760
+مكونة biofilm تتعاملش كـ single cells، بتتعامل كـ
+
+327
+00:24:56,760 --> 00:25:00,660
+multicellular organism، يعني كـ .. كـ .. كـ .. كـ ..
+
+328
+00:25:00,660 --> 00:25:05,860
+كائن ذو خلايا متعددة، يعني زي ده، إلا دماغ وإلا
+
+329
+00:25:05,860 --> 00:25:10,040
+عينين وإلا إيدين، فبتتصرف بهذا الـ .. بهذا السلوك،
+
+330
+00:25:10,040 --> 00:25:13,300
+وهذا التصرف بهذا السلوك بيؤدي إلى إن أدائها بيكون
+
+331
+00:25:13,300 --> 00:25:17,460
+أفضل إيش نقلدها أحسن؟ الـ survival تبعها في الـ
+
+332
+00:25:17,460 --> 00:25:21,800
+environment بيكون أفضل، مقاومتها للعوامل الجارحة،
+
+333
+00:25:21,800 --> 00:25:24,180
+يعني الـ injurious agents، الـ chemicals أو الحرارة،
+
+334
+00:25:24,180 --> 00:25:28,560
+بتكون أفضل من مما لو كانت single cell فحتى
+
+335
+00:25:28,560 --> 00:25:32,120
+مقاومتها للـ phagocytosis لإنّها تنتج المواد اللي
+
+336
+00:25:32,120 --> 00:25:36,100
+بتستخدمها لإنّها تربط حالة بعض، برضه عبارة عن
+
+337
+00:25:36,100 --> 00:25:39,830
+polysaccharide والـ polysaccharide برضه بيكون Mucoid
+
+338
+00:25:39,830 --> 00:25:44,510
+فبسهّل أو بصعّب عملية الـ phagocytosis وبسهّل
+
+339
+00:25:44,510 --> 00:25:49,510
+عملية الهروب بتتذكروا الـ lipopolysaccharide كان مكوّن
+
+340
+00:25:49,510 --> 00:25:52,650
+من الـ lipid A core polysaccharide O side chain الـ
+
+341
+00:25:52,650 --> 00:25:55,730
+O side chain على سبيل المثال في الـ lipopolysaccharide لبعض الـ
+
+342
+00:25:55,730 --> 00:25:58,990
+gram negative بكتيريا برضه
+
+343
+00:25:58,990 --> 00:26:02,670
+عامل مساعد على الهروب من الـ phagocytosis زي
+
+344
+00:26:02,670 --> 00:26:06,740
+الـ Escherichia coli ما هو ذكره عنها في K antigen و K
+
+345
+00:26:06,740 --> 00:26:12,060
+antigen هو حقيقة capsular antigen ويله مقابل في
+
+346
+00:26:12,060 --> 00:26:16,580
+الـ Salmonella typhi مسمى مشهور اسمه Vi antigen يبقى
+
+347
+00:26:16,580 --> 00:26:21,740
+الـ K و الـ Vi بيشبهوا بعض و كلاهما تعبير عن capsule
+
+348
+00:26:21,740 --> 00:26:25,300
+بس صغيرة microcapsule
+
+349
+00:26:26,890 --> 00:26:30,750
+برضه بيساعد على الهروب طبعا شوفوا التفاصيل و
+
+350
+00:26:30,750 --> 00:26:35,770
+الكلمات الطفيفة و اللي بتمر معنا مرور سريع بكرا
+
+351
+00:26:35,770 --> 00:26:39,670
+هتتذكرها هأعطيكم مثال صغير لإنّ عشان في الـ
+
+352
+00:26:39,670 --> 00:26:44,380
+diagnostic هتسمعوا الجملة دي اللي حاكيلكم الـ Escherichia
+
+353
+00:26:44,380 --> 00:26:48,300
+coli normal flora of the intestine لكن number one
+
+354
+00:26:48,300 --> 00:26:51,340
+cause of urinary tract infection البعض منها ممكن
+
+355
+00:26:51,340 --> 00:26:54,380
+يعمل neonatal meningitis إيش هي neonatal
+
+356
+00:26:54,380 --> 00:26:59,860
+meningitis؟ التهاب سحايا عند الأطفال، يحدثه
+
+357
+00:26:59,860 --> 00:27:06,740
+الولادة وورد إن كل العزلات كل العزلات اللي
+
+358
+00:27:07,440 --> 00:27:10,560
+بيعزلوها من الأطفال اللي عندهم neonatal meningitis
+
+359
+00:27:10,560 --> 00:27:14,220
+وورد إن الـ isolates السلالة يعني بتنتج K capsule
+
+360
+00:27:14,220 --> 00:27:17,760
+يبقى الـ K capsule هو اللي أهلها تعمل neonatal
+
+361
+00:27:17,760 --> 00:27:21,920
+meningitis وسط صورتها one single factor ووجد في
+
+362
+00:27:21,920 --> 00:27:25,440
+البكتيريا أعطاها قدرة أكثر منها من زميلاتها الأخريات
+
+363
+00:27:26,640 --> 00:27:31,180
+رقم ستة هو الرائع والجميل الحلو كثير اللي فيه فكرة
+
+364
+00:27:31,180 --> 00:27:36,680
+وفيه يعني إعجاز حقيقة نطلع على الـ antibody اللي
+
+365
+00:27:36,680 --> 00:27:40,860
+احنا شفناه جاب شوية الـ antibody بنفترض إنه بهذا
+
+366
+00:27:40,860 --> 00:27:44,740
+الشكل اللي antigen binding side يعني الـ antibody
+
+367
+00:27:44,740 --> 00:27:48,520
+هذا الطرف تبعه هي الـ antigen تبعه بيجي بيرتبط بهذا
+
+368
+00:27:48,520 --> 00:27:55,380
+الشكل الجهة الخلفية من الـ Antibody هذي بتشكل
+
+369
+00:27:55,380 --> 00:28:00,260
+receptor للـ phagocytes والـ macrophages فالآن البكتيريا
+
+370
+00:28:00,260 --> 00:28:04,060
+مرتبطة بهذا الشكل بالـ Antigen
+
+371
+00:28:12,920 --> 00:28:16,360
+مين بيجي يرتبط المفروض الـ macrophage ترتبط بهذا
+
+372
+00:28:16,360 --> 00:28:19,020
+الجزء يعني .. يعني .. يعني كل الـ macrophage و الـ
+
+373
+00:28:19,020 --> 00:28:21,720
+phagocytes اللي هي receptors في هذا الجزء بيسموه
+
+374
+00:28:21,720 --> 00:28:26,040
+Fc portion فلما تلجي ترتبط فيه حتى لو البكتيريا
+
+375
+00:28:26,040 --> 00:28:30,820
+إليها capsule حتى لو بتهرب من أي حاجة بنام صار جسر 
+
+376
+00:28:30,820 --> 00:28:34,180
+بينها و بين جسر اللي هو ال antibody بينها و بينها
+
+377
+00:28:34,180 --> 00:28:38,000
+بتلتقموا على طول فإيش الـ .. إيش البكتيريا بتسوي
+
+378
+00:28:38,000 --> 00:28:40,680
+هذا اللي اسمها Staphylococcus aureus بتنتج protein
+
+379
+00:28:40,680 --> 00:28:45,340
+اسمه protein إيه؟ هذا كمناك بروتيوم مش هتلاجمه، هذا
+
+380
+00:28:45,340 --> 00:28:47,500
+ممسك الانتبار يبقى برج طب يجلبه زيك
+
+381
+00:28:50,380 --> 00:28:53,640
+ويربط بنفس الـ .. الـ .. الـ .. الـ receptor تبع الـ
+
+382
+00:28:53,640 --> 00:28:57,140
+macrophage تمام؟ و الفاج و الـ size إيش بيصير فيه
+
+383
+00:28:57,140 --> 00:29:01,880
+فاجوزوم معلّق هنا؟ فاضي .. فاضي .. فاضي .. فاضي ..
+
+384
+00:29:01,880 --> 00:29:01,960
+فاضي .. فاضي .. فاضي .. فاضي .. فاضي .. فاضي ..
+
+385
+00:29:01,960 --> 00:29:02,140
+فاضي .. فاضي .. فاضي .. فاضي .. فاضي .. فاضي ..
+
+386
+00:29:02,140 --> 00:29:02,240
+فاضي .. فاضي .. فاضي .. فاضي .. فاضي .. فاضي ..
+
+387
+00:29:02,240 --> 00:29:05,120
+فاضي .. فاضي .. فاضي .. فاضي .. فاضي .. فاضي ..
+
+388
+00:29:05,120 --> 00:29:17,860
+فاضي .. فاضي .. فاضي .. فاضي
+
+389
+00:29:17,860 --> 00:29:22,770
+..الارتباط الـ normal للـ antigen أو للـ antibody هو
+
+390
+00:29:22,770 --> 00:29:26,270
+عبر الـ antigen binding site بهذا الشكل و على الـ طرف
+
+391
+00:29:26,270 --> 00:29:30,930
+التاني بيشكل receptor لمين؟ للـ macrophage الـ protein
+
+392
+00:29:30,930 --> 00:29:37,210
+A بياخد الـ FC portion هذا و بيرتبط فيه و بيخلي هذا
+
+393
+00:29:37,210 --> 00:29:41,630
+periphery وبالتالي لن يرتبط الـ macrophage أو الـ phagocyte
+
+394
+00:29:41,630 --> 00:29:46,710
+صحيح إذا هذه حركة ذكية من الـ Staphylococcus aureus
+
+395
+00:29:46,710 --> 00:29:51,410
+بإنها تعمل neutralization للـ antibody اللي ممكن
+
+396
+00:29:51,410 --> 00:29:56,870
+يسهل في عمل اللي هو الـ phagocytosis أعتقد أنه
+
+397
+00:29:56,870 --> 00:30:00,730
+أجهزت نفسي و أنا بشرح و أحاول بالجلم و هيفين صورة
+
+398
+00:30:00,730 --> 00:30:03,910
+على الـ صبورة فكويس أني أطلع عليها هاي
+
+399
+00:30:03,910 --> 00:30:09,210
+Staphylococcus مثلا Epidulis و هذه Aureus الثانية
+
+400
+00:30:09,930 --> 00:30:14,330
+لاحظوا الـ E-Dermatitis طبعا ما عندهاش protein A
+
+401
+00:30:14,330 --> 00:30:18,870
+فبالتالي هي اللي هو الـ .. الـ .. الـ .. الـ antibody
+
+402
+00:30:18,870 --> 00:30:22,830
+مرتبط بالـ antigen مرتبط بالـ antigen هنا وهي الـ
+
+403
+00:30:22,830 --> 00:30:26,390
+phagocytes المفروض مرتبط بمين لها؟ بالـ Fc .. في الـ
+
+404
+00:30:26,390 --> 00:30:29,850
+antibody في الـ antibody فبتكوّن un desert منهم
+
+405
+00:30:29,850 --> 00:30:32,990
+طلعوا لنا في الـ صورة هذه بتكوّن هل الـ macrophage
+
+406
+00:30:32,990 --> 00:30:36,570
+هي الـ desert؟ وهي الـ antibody طبعا الـ antibody هذا
+
+407
+00:30:36,570 --> 00:30:40,590
+ثنائي الشعبة اللي ما بيعرفوش في خماس الشعبة كمان بس
+
+408
+00:30:40,590 --> 00:30:44,310
+هذا ثنائي الشعبة أو يعني two antigen binding sites
+
+409
+00:30:44,310 --> 00:30:49,350
+ليش صار فندي هنا الـ macrophage مسكت اتنين بكتيريا
+
+410
+00:30:49,350 --> 00:30:53,530
+عبر one single antibody على طول الأقعد لو كان لهم
+
+411
+00:30:53,530 --> 00:30:55,950
+capsule بقى و مش capsule اللي هم إيش ما كان لهم
+
+412
+00:30:55,950 --> 00:30:59,790
+كان ساقونهم قرروا تنفيب أنهم ليش لقاعد حادث الخطوة
+
+413
+00:30:59,790 --> 00:31:01,850
+الأولى اللي هي الـ attachment الـ attachment جدا مهم
+
+414
+00:31:01,850 --> 00:31:06,020
+جدا في هذا الموضوع هذه لما بكتيريا بتنتج من روتين A
+
+415
+00:31:06,020 --> 00:31:08,880
+يجي لبكتيريا الثانية البكتيريا الثانية استفد ل
+
+416
+00:31:08,880 --> 00:31:12,340
+Cocas Aureus ومن النوع اللي بنتج من روتين A إيش
+
+417
+00:31:12,340 --> 00:31:16,580
+عمل روتين A؟ روتين A أخد الـ antibody و ارتبط فيه
+
+418
+00:31:16,580 --> 00:31:20,040
+هل له ريسيبترو للـ macrophage للـ phagocytes؟ ما لوش
+
+419
+00:31:20,040 --> 00:31:26,600
+ريسيبترو فبالتالي عمل تحييد للـ phagocytes وعمل تحييد
+
+420
+00:31:26,600 --> 00:31:30,940
+لـ macrophage من خلال ارتباطه بالـ Fc portion
+
+421
+00:31:31,160 --> 00:31:33,480
+اللي هو protein A و الـ protein A موجود على الـ
+
+422
+00:31:33,480 --> 00:31:37,160
+surface تبع الـ Staphylococcus aureus لذلك بنلاقي
+
+423
+00:31:37,160 --> 00:31:42,360
+احنا الـ pathogenicity تبع الـ Staphylococcus aureus
+
+424
+00:31:42,360 --> 00:31:45,020
+أعلى بكثير من الـ pathogenicity تبع الـ
+
+425
+00:31:45,020 --> 00:31:49,200
+Staphylococcus epigenesis و زي ما حتى كنا بقولها
+
+426
+00:31:49,200 --> 00:31:52,780
+non pathogenic بينما دي pathogenic
+
+427
+00:31:56,340 --> 00:32:00,080
+الـ M-protein اللي شايفينه قدامنا هنا بيشتغل برضه
+
+428
+00:32:00,080 --> 00:32:05,080
+بطريقة أنه يمنع اللي جاي عليه بيمنع الـ .. الـ .. الـ
+
+429
+00:32:05,080 --> 00:32:11,820
+phagocytosis و
+
+430
+00:32:11,820 --> 00:32:15,620
+بيعمل عملية تنشيط لمين؟ للـ complement و أنا هذكركم
+
+431
+00:32:15,620 --> 00:32:19,840
+بموضوع الـ complement complement مرة أخرى بنات هي
+
+432
+00:32:19,840 --> 00:32:25,900
+..جروب بروتين لكن هي non-active، الـ activation
+
+433
+00:32:25,900 --> 00:32:30,620
+تبعها بيحدث المطلوب إنه في مواطن محددة، يعني مثلا
+
+434
+00:32:30,620 --> 00:32:34,760
+شفتوا الارتباط اللي صار بين الـ Staphylococcus aureus
+
+435
+00:32:34,760 --> 00:32:36,160
+وبين الـ antibody؟
+
+436
+00:32:41,110 --> 00:32:46,650
+إذا صار ارتباط طبيعي لكل من الـ IgG والـ IgM، الـ IgG
+
+437
+00:32:46,650 --> 00:32:49,790
+والـ IgM بيعملوا activation للـ complement لما
+
+438
+00:32:49,790 --> 00:32:53,610
+تصير activation للـ complement، بيتكون في المحصلة
+
+439
+00:32:53,610 --> 00:32:58,230
+الـ activation مُركّب، مُعقد، بيسموه membrane
+
+440
+00:32:58,230 --> 00:33:02,810
+attack complex، هذا بلزق فيه الخلية البكتيرية
+
+441
+00:33:02,810 --> 00:33:08,660
+وبيعمل فيها حفرة، حفرة وبالتالي بيصير فيها إنها
+
+442
+00:33:08,660 --> 00:33:13,820
+محتويات الخلية إلى الخارج، بتموت البكتيريا، طيب
+
+443
+00:33:13,820 --> 00:33:17,180
+هذا الـ .. هذا الـ .. الـ .. الـ .. الـ activation صار
+
+444
+00:33:17,180 --> 00:33:20,760
+في خلية جنب الكلية أو في الكلية، هيدمر مين؟
+
+445
+00:33:20,760 --> 00:33:24,580
+هيدمر الـ nephrons، صح ولا لأ؟ صار في خلايا في الـ
+
+446
+00:33:24,580 --> 00:33:28,040
+blood vessels، هيدمر خلايا الـ epithelial cells،
+
+447
+00:33:28,040 --> 00:33:30,560
+يفجر الـ activation للـ complement في غير موضوعها
+
+448
+00:33:30,560 --> 00:33:32,380
+تأذّين لواصل
+
+449
+00:33:35,400 --> 00:33:40,180
+نجي لحاجة اسمها intracellular pathogenesis تخيلوا
+
+450
+00:33:40,180 --> 00:33:45,020
+هذا الموضوع كده أخدت مننا يعني حجم معلومات لو لأنه
+
+451
+00:33:45,020 --> 00:33:50,340
+مهم وبالنسبة إنه بنعتبره يعني basic knowledge كان
+
+452
+00:33:50,340 --> 00:33:55,710
+احنا مركزنا عليه كثير احنا درسنا two bacterias
+
+453
+00:33:55,710 --> 00:34:00,670
+بالجمع هذه الـ bacterias اللي هم Chlamydia and
+
+454
+00:34:00,670 --> 00:34:05,310
+Rickettsia وسمّيناهم obligate intracellular bacteria
+
+455
+00:34:05,310 --> 00:34:09,750
+إيش يعني obligate intracellular يعني اصلا ما بقدروش
+
+456
+00:34:09,750 --> 00:34:14,650
+يعيشوا free عكسها free living عكس الـ obligate free
+
+457
+00:34:14,650 --> 00:34:19,030
+living خدوا بالكم من الـ مميز بين obligate
+
+458
+00:34:19,030 --> 00:34:22,370
+intracellular وبين facultative intracellular
+
+459
+00:34:22,370 --> 00:34:25,750
+facultative يعني بتقدر تعيش معايشة حرة وبتقدر تعيش
+
+460
+00:34:25,750 --> 00:34:33,050
+معايشة .. طب تخيلوا اتنين بيعيشوا داخل خلايانا
+
+461
+00:34:33,050 --> 00:34:38,270
+معنى الحكي عندهم المقدرة على الهروب من عملية القتل
+
+462
+00:34:38,270 --> 00:34:40,990
+اللي احنا بسمّيها intracellular
+
+463
+00:34:43,720 --> 00:34:47,100
+احنا برضه يعني الـ background اللي موجودة عند
+
+464
+00:34:47,100 --> 00:34:51,360
+المجموعة مش كلهم واحدة فمضطرين ننزل لبعض الـ .. الـ
+
+465
+00:34:51,360 --> 00:34:56,240
+.. المعلومات إلى مستوى عقلي فاكرين الـ phagocyte
+
+466
+00:34:56,240 --> 00:34:59,500
+اللي بيقول إن أنا بتلتقم .. التقمت البكتيريا؟
+
+467
+00:34:59,500 --> 00:35:02,440
+التقمتها إيش عملت فيها؟ بس أخدتها من برا و أخدتها
+
+468
+00:35:02,440 --> 00:35:06,020
+جوا؟ أولا طبعا مش أدفع تأثيراتك الآن بتحطها هي في
+
+469
+00:35:06,020 --> 00:35:09,700
+physical جوا و زي العجز ميروتكو بتصب عليها بعض
+
+470
+00:35:09,700 --> 00:35:14,860
+الأنزيمات تمام؟ الهاضم والمحللة و بتكسرها وإذا كانت
+
+471
+00:35:14,860 --> 00:35:18,360
+macrophage الـ macrophage هي الرجل الحكيم أو الـ
+
+472
+00:35:18,360 --> 00:35:22,680
+wise man للـ immune system تختار منها جطع fragments
+
+473
+00:35:22,680 --> 00:35:26,840
+منها و بتعملها presentation لجهاز المناعة عشان
+
+474
+00:35:26,840 --> 00:35:29,840
+جهاز المناعة يكون لها جسام مضاد سموها antigen
+
+475
+00:35:29,840 --> 00:35:32,320
+presenting cell إن شاء الله لما ندرس المناعة بإذن
+
+476
+00:35:32,320 --> 00:35:35,360
+الله هدرس المعلومة لكن لا بأس إنه يكون عندنا بعض
+
+477
+00:35:35,360 --> 00:35:40,560
+المصطلحات كده و أنا وصلنا احنا وصلنا إن الـ
+
+478
+00:35:40,560 --> 00:35:47,240
+phagocyte أخدت البكتيريا على أي أساس على أساس أنه
+
+479
+00:35:47,240 --> 00:35:55,600
+تقتلها إذا في عملية نية مسبقة للقتل مع صدق الإصرار
+
+480
+00:35:55,600 --> 00:36:02,620
+والتّرصد طيب هناك بعض البكتيريا بعض البكتيريا عندها
+
+481
+00:36:02,620 --> 00:36:08,760
+المقدرة أن تدخل داخل الخلايا وتتلافى هذا التعمد في
+
+482
+00:36:08,760 --> 00:36:10,600
+القتل يبقى بيكون عندها
+
+483
+00:36:16,610 --> 00:36:21,670
+الخلايا تبقى حقيقية كيف؟ some bacteria live and
+
+484
+00:36:21,670 --> 00:36:24,050
+grow within polymorphonuclear cells طبعا الـ
+
+485
+00:36:24,050 --> 00:36:27,770
+polymorphonuclear cells لاسم ثاني PMNs وهي الـ
+
+486
+00:36:27,770 --> 00:36:31,650
+neutrophil وهي الـ phagocyte يعني الـ
+
+487
+00:36:31,650 --> 00:36:36,070
+polymorphonuclear cells هي نفسها الـ phagocyte اسم
+
+488
+00:36:36,070 --> 00:36:38,090
+ثاني لمين؟ لـ neutrophils
+
+489
+00:36:40,350 --> 00:36:44,970
+macrophages or monocytes by وحد avoiding entry
+
+490
+00:36:44,970 --> 00:36:48,290
+into phagolysis هون طيب بعد إذن الأستاذ خالد إذا
+
+491
+00:36:48,290 --> 00:36:54,070
+في كلام بس اللي بتبقى سبورا احنا اتفقنا إنه الـ
+
+492
+00:36:54,070 --> 00:37:03,490
+phagocytes أخدت البتيريا عبر extension لزي سودو
+
+493
+00:37:03,490 --> 00:37:07,290
+بوديا بعد إن سكرتهم هدول و بالآخر البتيريا صارت
+
+494
+00:37:07,290 --> 00:37:16,550
+بهذا الشكل هذا الجزء اللي زمناه فاقوزوم فاقوزوم
+
+495
+00:37:16,550 --> 00:37:23,490
+لأن هو الجسم اللي صار فيه الخلية البكتيريا المنوي
+
+496
+00:37:23,490 --> 00:37:28,950
+قتله بيجعلنا حادث منها من جوا الخلية اللايسوزوم
+
+497
+00:37:28,950 --> 00:37:32,790
+اللايسوزوم
+
+498
+00:37:32,790 --> 00:37:36,330
+يفترض في الـ normal killing أنه يرتبط في الـ phagosome
+
+499
+00:37:36,950 --> 00:37:43,210
+وبتكون عندنا ما يسمى بـ Phagolysosome يعني خلط ما
+
+500
+00:37:43,210 --> 00:37:47,810
+بين two particles اللي هم الـ phagosome والـ lysosome، 
+
+501
+00:37:47,810 --> 00:37:54,050
+بعدين حضرة الليسوزوم، ولحظوا الليسوزوم لن لن يفرغ
+
+502
+00:37:54,050 --> 00:37:58,330
+محتوياته في الخلية بشكل حر لأنه هيدمر الخلية،
+
+503
+00:37:58,330 --> 00:38:02,470
+بيفرغها فقط في حاجة إلها من الـ brain داخل الخلية،
+
+504
+00:38:02,470 --> 00:38:05,730
+زي مين؟ زي الفاجو، بالظبط زي الفاجوزوم،
+
+505
+00:38:05,730 --> 00:38:10,850
+البكتيريا تبدأ إيش؟ تتكسر و تتحلل و كما قلت لك إذا 
+
+506
+00:38:10,850 --> 00:38:14,130
+كانت مكروه فارغ بيصير لها presentation الآخر، الآن
+
+507
+00:38:14,130 --> 00:38:21,170
+كيف ممكن بكتيريا تعيش جوا؟
+
+508
+00:38:21,170 --> 00:38:27,050
+هاي الفاجوزوم صار جوا، الخطب تنشر الآن، احنا قلنا
+
+509
+00:38:27,050 --> 00:38:31,130
+أن الفاجوزوم يرتبط، في بكتيريا بتنتج chemical و
+
+510
+00:38:31,130 --> 00:38:34,270
+هي جوازي الـ chemicals اللي انتجتها البكتيريا و
+
+511
+00:38:34,270 --> 00:38:40,050
+جالتها الفاجوسايت فالس أرم ارجع تمنع الفاجو أو
+
+512
+00:38:40,050 --> 00:38:45,010
+تمنع الليزوم من أنه مش أنه يرتبط أنه يجعلها المنطقة
+
+513
+00:38:45,010 --> 00:38:50,990
+أساسا تمام؟ بتكسر له الطريق تبعه ممنوع بكتيريا 
+
+514
+00:38:50,990 --> 00:38:56,770
+أخرى بكتيريا أخرى ممكن تسمح للليزوم يرتبط لكن
+
+515
+00:38:56,770 --> 00:39:01,790
+تسمح لهش أن يعمل fusion no fusion يعني مافيش إيه؟
+
+516
+00:39:01,830 --> 00:39:06,870
+عملية ابتحاد ..عملية ابتحاد وفيه بكتيريا are
+
+517
+00:39:06,870 --> 00:39:12,370
+resistant .. resistant to lysosomal destruction
+
+518
+00:39:12,370 --> 00:39:18,310
+يعني بتصب عليها الانزيمات وكأنه بتصب عليها حاجة
+
+519
+00:39:18,310 --> 00:39:21,630
+مؤذية، عاج، تمام؟ بتهيئنا من دلوقتي، إذا في
+
+520
+00:39:21,630 --> 00:39:25,890
+مجموعة منها .. من .. وفي بنات كمان بكتيريا ذكية،
+
+521
+00:39:25,890 --> 00:39:29,710
+إيش بتعمل؟ وهي .. و هي جوا، قبل ما الـ lysosome 
+
+522
+00:39:29,710 --> 00:39:30,910
+يجي بتحلل
+
+523
+00:39:33,600 --> 00:39:39,200
+الفاجوزوم إذا حلت الفاجوزوم هالحضرته من الغباء من
+
+524
+00:39:39,200 --> 00:39:43,700
+الغباء أنه يكف محتوياته على البكتيريا داخل الخلية
+
+525
+00:39:43,700 --> 00:39:49,440
+هيموت فخلاص بس كيف بيبطل له أي تأثير في هذه الحب
+
+526
+00:39:49,440 --> 00:39:55,460
+إذا فإن مجموعة من الحيل اللي ممكن تلجأ إلها
+
+527
+00:39:55,460 --> 00:39:59,680
+البكتيريا للبقاء حية خدوا بالكوا قد تنتمي إلى واحد
+
+528
+00:39:59,680 --> 00:40:04,160
+من الاتنين obligate intracellular يا إما الـ facultative
+
+529
+00:40:04,160 --> 00:40:10,000
+الـ Intracellular من الميكروبات اللي بتمتلك مقدرة
+
+530
+00:40:10,000 --> 00:40:12,380
+على Intracellular survival اللي هي الـ
+
+531
+00:40:12,380 --> 00:40:16,900
+mycobacterium tuberculosis المسببة للمرض الخطير
+
+532
+00:40:16,900 --> 00:40:22,060
+المعروف عالميا باسم tuberculosis أو الـ TB وكان هنا
+
+533
+00:40:22,060 --> 00:40:26,160
+في فلسطين منتشر بشكل كبير وكان له مستشفى خاص في
+
+534
+00:40:26,160 --> 00:40:29,940
+منطقة البراج في معسكر البراج سكان البراج مين من
+
+535
+00:40:29,940 --> 00:40:37,210
+سكان البراج؟حدش؟ في منطقة خلوية إلى الشرق أعتقد
+
+536
+00:40:37,210 --> 00:40:41,510
+أنها أصبحت مدرسة الآن أو إشي كان في مستشفى اسمه
+
+537
+00:40:41,510 --> 00:40:44,350
+مستشفى الـ TB معروف أن عند الناس أن المستشفى الـ TB
+
+538
+00:40:44,350 --> 00:40:47,290
+أو الـ tuberculosis و أغلق يمكن في الـ .. في أواخر
+
+539
+00:40:47,290 --> 00:40:51,810
+السبعينات لأنه بدأ عدد اللي إيه الحالات يقل بيتحسن
+
+540
+00:40:51,810 --> 00:40:55,470
+السبعينات واحدة ممكن نعرف في أواخر السبعينات احنا
+
+541
+00:40:55,470 --> 00:40:58,650
+ممكن نعرف؟ لأ مش جدا اللي تعرف يعني تسمع من .. من
+
+542
+00:40:58,650 --> 00:41:04,870
+الأجيال اللي .. طيب الـ Mycobacterium leprae اللي هو
+
+543
+00:41:04,870 --> 00:41:08,670
+بسببه الليبروسي .. بسبب بعض الليبروسي اللي هو
+
+544
+00:41:08,670 --> 00:41:11,630
+الجذام .. بعض الجذام .. برضه obligate .. هذه
+
+545
+00:41:11,630 --> 00:41:14,690
+obligate intracellular صحيح .. الأولى facultative
+
+546
+00:41:14,690 --> 00:41:19,470
+الـ Listeria monocytogenes و هذه من أروع الميكروبات
+
+547
+00:41:19,470 --> 00:41:25,030
+اللي ممكن تشوفوها في الـ .. داخل الخلايا هذه
+
+548
+00:41:25,030 --> 00:41:28,170
+البكتيريا عندما تنتقل من خلية إلى خلية تخرج من
+
+549
+00:41:28,170 --> 00:41:34,540
+الخلية الأولى تنتقل عبر جدار الخلوي ما بين التنتين
+
+550
+00:41:34,540 --> 00:41:38,760
+الخلية الـ .. الـ infected الـ bacterial cells وهي
+
+551
+00:41:38,760 --> 00:41:43,540
+خلية أخرى مجاورا إلها تمام؟ خلية مجاورا إلها هاي
+
+552
+00:41:43,540 --> 00:41:51,380
+الـ Listeria monocytogenes الـ Listeria متحركة لكنش بتعمل
+
+553
+00:41:51,380 --> 00:41:58,220
+تستغل الأكتين اللي موجود في الخلية و بتعمله
+
+554
+00:41:58,220 --> 00:41:59,220
+polymerization
+
+555
+00:42:02,630 --> 00:42:06,030
+ماذا يعني Polymerization؟ يعني هو فك فك تمام؟
+
+556
+00:42:06,030 --> 00:42:09,330
+فبتملق .. دي Polymerize بسموه لإن هو بتملق
+
+557
+00:42:09,330 --> 00:42:15,650
+Polymerization فبتكون خيط أو خيوط من الأكتين و
+
+558
+00:42:15,650 --> 00:42:18,630
+الأكتين مادة هي اللي عاملة الـ cytoskeleton تبع
+
+559
+00:42:18,630 --> 00:42:22,470
+الخلية يعني هي اللي رافعة السجف و لامدان يعني هي
+
+560
+00:42:22,470 --> 00:42:28,110
+عاملة الخلية الـ cytoskeleton فبتروح و هي الأكتين
+
+561
+00:42:28,110 --> 00:42:31,450
+اللي بيعملها Polymerization بتحركها و كإنها صاروخ
+
+562
+00:42:31,950 --> 00:42:36,290
+فبتروح اللي هي ضاربة من الجدار تبقى الخلية بهذا
+
+563
+00:42:36,290 --> 00:42:42,430
+الشكل ومنتقلة لمين؟ الخلية الأخرى وبيسموا الـ .. الـ
+
+564
+00:42:42,430 --> 00:42:45,670
+.. الـ mechanism actin rockets، rockets يعني
+
+565
+00:42:45,670 --> 00:42:49,630
+صواريخ، صواريخ من الأكتين، أنه الأكتين هو اللي
+
+566
+00:42:49,630 --> 00:42:52,770
+بيدفعها إلى اللي هو الـ .. الـ .. الـ .. المنطقة
+
+567
+00:42:52,770 --> 00:42:57,630
+الأخرى و الخلية الأخرى وبالسبب لستريوسز و طبعا كتير
+
+568
+00:42:57,630 --> 00:43:00,510
+من الـ .. من الـ .. من الـ infection زبانات احنا يعني
+
+569
+00:43:00,510 --> 00:43:05,690
+بنعطيها oses، تمام؟ tuberculosis،
+
+570
+00:43:05,690 --> 00:43:09,490
+salmonellosis، shigellosis، تمام؟ shigellosis،
+
+571
+00:43:09,490 --> 00:43:12,870
+فقد نعطيها اسم مرض زي مثلا الـ salmonella، لأ ممكن
+
+572
+00:43:12,870 --> 00:43:16,750
+نقول typhoid fever، وبنفس الوقت نقول salmonella
+
+573
+00:43:16,750 --> 00:43:20,960
+osis زي ما شوفنا هانا هانا سنة لطيفة ايفاويفيبر
+
+574
+00:43:20,960 --> 00:43:24,000
+الـ Shigella dysenteriae اللي اعطينا الفاسيليا الفاسيليا
+
+575
+00:43:24,000 --> 00:43:26,840
+الديزنتيريا الـ Yersinia pestis الـ plague اللي هو
+
+576
+00:43:26,840 --> 00:43:30,380
+الطاعون دار المرض معانا الـ Brucella species اللي هو
+
+577
+00:43:30,380 --> 00:43:33,740
+بسبب البروسيلوسس اللي هي الحمّة الملطية و
+
+578
+00:43:33,740 --> 00:43:36,000
+الـ Haemophilus influenzae اللي هو بسبب النمونيا
+
+579
+00:43:36,000 --> 00:43:39,280
+و الـ Rickettsia و الـ Chlamydia اللي أنا ذكرتهم أول أش
+
+580
+00:43:39,280 --> 00:43:41,580
+هدولة التنتين هم الوحيدين اللي بنادوا ولعبناها
+
+581
+00:43:42,140 --> 00:43:46,000
+obligate, intra, cellular, بلا صحيح اللي هو الـ ..
+
+582
+00:43:46,000 --> 00:43:48,660
+الـ .. الـ biochemistry الـ biochemistry أستاذ مش
+
+583
+00:43:48,660 --> 00:43:52,400
+قلتي التانية obligated؟ اللي هم التانية؟ التانية
+
+584
+00:43:52,400 --> 00:43:57,380
+الـ Mycobacterium leprae هؤلاء هم الـ .. الـ ..
+
+585
+00:43:57,380 --> 00:44:00,720
+التنتين الـ well proven well proven هذه لإنه ما
+
+586
+00:44:00,720 --> 00:44:04,520
+عزلنهاش لحد الآن فقد يكون هناك ضمن إنها تكون
+
+587
+00:44:04,520 --> 00:44:08,500
+obligate تمام؟ كده قولت لكن .. لكن اللي مؤكد اللي
+
+588
+00:44:08,500 --> 00:44:12,870
+هم الـ .. اللي جيت ساوي اللي .. اللي كلمتطب نكمل إن
+
+589
+00:44:12,870 --> 00:44:14,950
+شاء الله المرة القادمة بيكون المحاضرة اللي بتاعة
+
+590
+00:44:14,950 --> 00:44:16,090
+هنا هي في الـ .. 
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/NQosy7xELT8_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/NQosy7xELT8_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..77e70b70df76e363b2327e3326c25a7e52ce527d
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/NQosy7xELT8_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 4503, "start": 20.79, "end": 45.03, "text": "بسم الله الرحمن الرحيم الموضوع اليوم ما زلنا فيه او اكمال لما يتعلق بالبعض genesis of بكتيريا ويمكن المحاضرة السابقة ركزنا فيها اكتر شي على ال exotoxins اليوم هيكون تركيزنا اكتر على ال endotoxin و بعدين هنعمل مقارنة بين ال exotoxin و ال endotoxin ونشوف ايش الفروقات الرئيسية ما بينهم بشكل ملخص", "tokens": [3555, 38251, 21984, 34892, 5016, 27842, 34892, 5016, 32640, 9673, 2407, 11242, 45367, 45595, 20498, 19446, 30767, 1211, 8315, 8978, 3224, 1975, 2407, 1975, 24793, 6027, 5296, 15042, 7251, 2655, 30241, 4587, 20666, 3555, 3615, 11242, 1049, 9374, 295, 4724, 4117, 2655, 13546, 25528, 4032, 32640, 19452, 9673, 5016, 46958, 25720, 21136, 16758, 28671, 12602, 4117, 11622, 8315, 8978, 11296, 1975, 4117, 2655, 2288, 44049, 15844, 2423, 454, 310, 5230, 1292, 45595, 20498, 39896, 30544, 6055, 31747, 1829, 11622, 8315, 1975, 4117, 2655, 2288, 15844, 2423, 917, 310, 5230, 259, 4032, 39182, 9957, 8032, 1863, 25957, 1211, 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{"start": 128.48, "end": 128.66, "word": " على", "probability": 0.41259765625}, {"start": 128.66, "end": 128.82, "word": " ال", "probability": 0.921875}, {"start": 128.82, "end": 129.14, "word": " pre", "probability": 0.67236328125}, {"start": 129.14, "end": 129.9, "word": "-antigen", "probability": 0.80224609375}, {"start": 129.9, "end": 130.12, "word": " ممكن", "probability": 0.952880859375}, {"start": 130.12, "end": 130.44, "word": " نزيد", "probability": 0.9900716145833334}, {"start": 130.44, "end": 130.68, "word": " من", "probability": 0.9599609375}, {"start": 130.68, "end": 130.8, "word": " ال", "probability": 0.955078125}, {"start": 130.8, "end": 131.62, "word": " antigenicity", "probability": 0.8870442708333334}, {"start": 131.62, "end": 132.26, "word": " تبعه", "probability": 0.707763671875}], "temperature": 1.0}, {"id": 6, "seek": 16101, "start": 134.27, "end": 161.01, "text": "في المحصلة النهائية اتفقنا ان ال life of polysaccharide هو poor انتجين بالمقارنة مع اللي هو ال exotoxin اللي طبيعي تكوينه أصلا protein وهذا السبب الرئيسي في الفرق بين الاتنين ال protein is more antigenic than اللي هو ال polysaccharide ال endotoxin من أهم ال effects تبعته انه بيعملنا fever", "tokens": [41185, 9673, 5016, 9381, 37977, 28239, 3224, 16373, 10632, 1975, 2655, 5172, 4587, 8315, 16472, 2423, 993, 295, 6754, 82, 326, 7374, 482, 31439, 4716, 16472, 2655, 7435, 9957, 20666, 2304, 4587, 9640, 1863, 3660, 20449, 13672, 1829, 31439, 2423, 454, 310, 5230, 259, 13672, 1829, 23032, 21292, 3615, 1829, 6055, 4117, 2407, 9957, 3224, 5551, 9381, 15040, 7944, 37037, 15730, 21136, 3555, 3555, 34892, 19986, 1829, 3794, 1829, 8978, 27188, 2288, 4587, 49374, 2423, 9307, 1863, 9957, 2423, 7944, 307, 544, 2511, 3213, 299, 813, 13672, 1829, 31439, 2423, 6754, 82, 326, 7374, 482, 2423, 917, 310, 5230, 259, 9154, 5551, 16095, 2423, 5065, 6055, 3555, 34268, 3224, 16472, 3224, 4724, 1829, 25957, 1211, 8315, 18277], "avg_logprob": -0.20272775600522253, "compression_ratio": 1.716, 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"probability": 0.70355224609375}, {"start": 138.93, "end": 139.77, "word": " بالمقارنة", "probability": 0.9751790364583334}, {"start": 139.77, "end": 139.97, "word": " مع", "probability": 0.96923828125}, {"start": 139.97, "end": 140.13, "word": " اللي", "probability": 0.5439453125}, {"start": 140.13, "end": 140.43, "word": " هو", "probability": 0.9697265625}, {"start": 140.43, "end": 140.67, "word": " ال", "probability": 0.91162109375}, {"start": 140.67, "end": 141.31, "word": " exotoxin", "probability": 0.72698974609375}, {"start": 141.31, "end": 141.49, "word": " اللي", "probability": 0.855712890625}, {"start": 141.49, "end": 141.75, "word": " طبيعي", "probability": 0.8099365234375}, {"start": 141.75, "end": 142.23, "word": " تكوينه", "probability": 0.88857421875}, {"start": 142.23, "end": 142.69, "word": " أصلا", "probability": 0.82421875}, {"start": 142.69, "end": 143.55, "word": " protein", "probability": 0.55322265625}, {"start": 143.55, "end": 144.55, "word": " وهذا", "probability": 0.852294921875}, {"start": 144.55, "end": 144.97, "word": " السبب", "probability": 0.9908854166666666}, {"start": 144.97, "end": 145.77, "word": " الرئيسي", "probability": 0.9919921875}, {"start": 145.77, "end": 146.07, "word": " في", "probability": 0.81201171875}, {"start": 146.07, "end": 146.47, "word": " الفرق", "probability": 0.98046875}, {"start": 146.47, "end": 146.67, "word": " بين", "probability": 0.96923828125}, {"start": 146.67, "end": 147.13, "word": " الاتنين", "probability": 0.750732421875}, {"start": 147.13, "end": 147.59, "word": " ال", "probability": 0.89501953125}, {"start": 147.59, "end": 148.03, "word": " protein", "probability": 0.9501953125}, {"start": 148.03, "end": 148.27, "word": " is", "probability": 0.91015625}, {"start": 148.27, "end": 148.65, "word": " more", "probability": 0.96240234375}, {"start": 148.65, "end": 149.51, "word": " antigenic", "probability": 0.88525390625}, {"start": 149.51, "end": 149.81, "word": " than", "probability": 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160.31, "end": 160.75, "word": " بيعملنا", "probability": 0.95634765625}, {"start": 160.75, "end": 161.01, "word": " fever", "probability": 0.93505859375}], "temperature": 1.0}, {"id": 7, "seek": 19144, "start": 162.84, "end": 191.44, "text": "و بيسموه pyrogen طلع الرقم اللي بدأ اتكلمكوا عنه الان واحد microgram لكل kilogram كافين انه يعملوا حرارة عند المرض واحد micro .. نتكلم عن microgram .. micro .. نتكلمش عن milligram .. نتكلم عن microgram طيب هدر الفبر .. الان لو طلعنا على ال effect التاني اللي اسمه disseminated intravascular coagulation", "tokens": [2407, 4724, 1829, 38251, 2407, 3224, 10664, 7747, 23032, 1211, 3615, 34892, 4587, 2304, 13672, 1829, 47525, 10721, 1975, 2655, 4117, 19528, 4117, 14407, 18871, 3224, 2423, 7649, 36764, 24401, 4532, 1342, 5296, 28820, 21741, 9122, 31845, 9957, 16472, 3224, 7251, 25957, 1211, 14407, 11331, 2288, 9640, 3660, 43242, 9673, 43042, 36764, 24401, 4532, 4386, 8717, 2655, 4117, 19528, 18871, 4532, 1342, 4386, 4532, 4386, 8717, 2655, 4117, 19528, 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{"start": 801.33, "end": 801.77, "word": " لل", "probability": 0.93701171875}, {"start": 801.77, "end": 802.29, "word": " antibiotic", "probability": 0.908203125}, {"start": 802.29, "end": 802.81, "word": " لكان", "probability": 0.4244384765625}, {"start": 802.81, "end": 803.17, "word": " صعب", "probability": 0.9962565104166666}, {"start": 803.17, "end": 803.49, "word": " تعمل", "probability": 0.99072265625}, {"start": 803.49, "end": 803.99, "word": " resistance", "probability": 0.9736328125}, {"start": 803.99, "end": 804.91, "word": " لمن؟", "probability": 0.8665364583333334}, {"start": 804.91, "end": 805.33, "word": " ل", "probability": 0.73291015625}, {"start": 805.33, "end": 805.43, "word": " ..", "probability": 0.58349609375}, {"start": 805.43, "end": 805.59, "word": " اللي", "probability": 0.965576171875}, {"start": 805.59, "end": 805.85, "word": " هو", "probability": 0.9853515625}, {"start": 805.85, "end": 806.11, "word": " ال", "probability": 0.90185546875}, {"start": 806.11, 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"probability": 0.5456136067708334}, {"start": 839.85, "end": 841.71, "word": " بيعمل", "probability": 0.9736328125}, {"start": 841.71, "end": 842.55, "word": " بينما", "probability": 0.828857421875}, {"start": 842.55, "end": 842.91, "word": " ال", "probability": 0.97265625}, {"start": 842.91, "end": 843.45, "word": " exoxin", "probability": 0.85400390625}, {"start": 843.45, "end": 843.59, "word": " مش", "probability": 0.27490234375}, {"start": 843.59, "end": 843.93, "word": " كان", "probability": 0.9033203125}, {"start": 843.93, "end": 845.25, "word": " ..", "probability": 0.493896484375}, {"start": 845.25, "end": 846.13, "word": " مابيعملش", "probability": 0.8461100260416666}, {"start": 846.13, "end": 846.63, "word": " غالب", "probability": 0.7515462239583334}, {"start": 846.63, "end": 846.95, "word": " لكن", "probability": 0.224365234375}, {"start": 846.95, "end": 847.25, "word": " هنا", "probability": 0.88232421875}, {"start": 847.25, "end": 847.31, "word": " ..", "probability": 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"probability": 0.7418212890625}, {"start": 2640.64, "end": 2640.72, "word": " ما", "probability": 0.9462890625}, {"start": 2640.72, "end": 2641.4, "word": " عزلنهاش", "probability": 0.8084716796875}, {"start": 2641.4, "end": 2641.76, "word": " لحد", "probability": 0.986083984375}, {"start": 2641.76, "end": 2642.22, "word": " الآن", "probability": 0.8095703125}, {"start": 2642.22, "end": 2642.98, "word": " فقد", "probability": 0.979248046875}, {"start": 2642.98, "end": 2643.26, "word": " يكون", "probability": 0.990966796875}, {"start": 2643.26, "end": 2643.62, "word": " هناك", "probability": 0.983154296875}, {"start": 2643.62, "end": 2643.96, "word": " ضمن", "probability": 0.57080078125}, {"start": 2643.96, "end": 2644.26, "word": " إنها", "probability": 0.801025390625}, {"start": 2644.26, "end": 2644.52, "word": " تكون", "probability": 0.99169921875}, {"start": 2644.52, "end": 2644.98, "word": " obligate", "probability": 0.87841796875}, {"start": 2644.98, "end": 2645.46, "word": " تمام؟", "probability": 0.7547200520833334}, {"start": 2645.46, "end": 2645.86, "word": " كده", "probability": 0.6653645833333334}, {"start": 2645.86, "end": 2646.06, "word": " قولت", "probability": 0.6184895833333334}, {"start": 2646.06, "end": 2646.26, "word": " لكن", "probability": 0.8896484375}, {"start": 2646.26, "end": 2646.38, "word": " ..", "probability": 0.93212890625}, {"start": 2646.38, "end": 2646.7, "word": " لكن", "probability": 0.97021484375}, {"start": 2646.7, "end": 2647.4, "word": " اللي", "probability": 0.902099609375}, {"start": 2647.4, "end": 2648.22, "word": " مؤكد", "probability": 0.9779052734375}, {"start": 2648.22, "end": 2648.5, "word": " اللي", "probability": 0.958984375}, {"start": 2648.5, "end": 2648.68, "word": " هم", "probability": 0.652587890625}, {"start": 2648.68, "end": 2648.96, "word": " ال", "probability": 0.5087890625}, {"start": 2648.96, "end": 2648.96, "word": " ..", "probability": 0.60302734375}, {"start": 2648.96, "end": 2649.2, "word": " اللي", "probability": 0.799072265625}, {"start": 2649.2, "end": 2649.44, "word": " جيت", "probability": 0.302734375}, {"start": 2649.44, "end": 2649.82, "word": " ساوي", "probability": 0.665283203125}, {"start": 2649.82, "end": 2650.16, "word": " اللي", "probability": 0.864013671875}, {"start": 2650.16, "end": 2650.16, "word": " ..", "probability": 0.4208984375}, {"start": 2650.16, "end": 2650.4, "word": " اللي", "probability": 0.9365234375}, {"start": 2650.4, "end": 2650.64, "word": " كلمت", "probability": 0.54248046875}], "temperature": 1.0}, {"id": 105, "seek": 265609, "start": 2651.87, "end": 2656.09, "text": "طب نكمل ان شاء الله المرة القادمة بيكون المحاضرة اللي بتاعة هنا هي في ال ..", "tokens": [9566, 3555, 8717, 24793, 1211, 16472, 13412, 16606, 21984, 9673, 25720, 25062, 18513, 46007, 4724, 1829, 30544, 9673, 5016, 46958, 25720, 13672, 1829, 39894, 995, 27884, 34105, 39896, 8978, 2423, 4386], "avg_logprob": -0.35717774741351604, "compression_ratio": 1.3854166666666667, "no_speech_prob": 0.0, "words": [{"start": 2651.87, "end": 2652.35, "word": "طب", "probability": 0.539306640625}, {"start": 2652.35, "end": 2652.83, "word": " نكمل", "probability": 0.97900390625}, {"start": 2652.83, "end": 2652.87, "word": " ان", "probability": 0.68798828125}, {"start": 2652.87, "end": 2653.03, "word": " شاء", "probability": 0.906982421875}, {"start": 2653.03, "end": 2653.11, "word": " الله", "probability": 0.9541015625}, {"start": 2653.11, "end": 2653.25, "word": " المرة", "probability": 0.890380859375}, {"start": 2653.25, "end": 2653.59, "word": " القادمة", "probability": 0.96728515625}, {"start": 2653.59, "end": 2653.87, "word": " بيكون", "probability": 0.7010091145833334}, {"start": 2653.87, "end": 2654.65, "word": " المحاضرة", "probability": 0.77935791015625}, {"start": 2654.65, "end": 2654.71, "word": " اللي", "probability": 0.789306640625}, {"start": 2654.71, "end": 2654.95, "word": " بتاعة", "probability": 0.4049479166666667}, {"start": 2654.95, "end": 2655.11, "word": " هنا", "probability": 0.400390625}, {"start": 2655.11, "end": 2655.35, "word": " هي", "probability": 0.6455078125}, {"start": 2655.35, "end": 2655.59, "word": " في", "probability": 0.86181640625}, {"start": 2655.59, "end": 2655.79, "word": " ال", "probability": 0.7392578125}, {"start": 2655.79, "end": 2656.09, "word": " ..", "probability": 0.8876953125}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 2657.2915, "duration_after_vad": 2517.5518749999887}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/OeCcJ-QHmoY_postprocess.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/OeCcJ-QHmoY_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..37bc1e62e2812efc855c6fb8bbb0f5de950ce3dc
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/OeCcJ-QHmoY_postprocess.srt
@@ -0,0 +1,1676 @@
+1
+00:00:20,700 --> 00:00:23,180
+بسم الله الرحمن الرحيم استكمارا اللي ما بدأناها
+
+2
+00:00:23,180 --> 00:00:26,260
+هتكلم اليوم ان شاء الله عن ال viral proteins وعن
+
+3
+00:00:26,260 --> 00:00:29,880
+ال viral envelope بالاضافة إلى ال atypical virus
+
+4
+00:00:29,880 --> 00:00:33,860
+-like agents مثل ال viroids ومثل ال defective
+
+5
+00:00:33,860 --> 00:00:36,700
+viruses و ال pseudovirons و ال pyruvas نبدأ بال
+
+6
+00:00:36,700 --> 00:00:41,960
+viral proteins طبعا احنا حاطين عنوان واسعة اسمه
+
+7
+00:00:41,960 --> 00:00:44,240
+viral proteins يعني كل واحد منهم ممكن ياخد مننا
+
+8
+00:00:44,240 --> 00:00:48,740
+محاضرة لكن حابين انه نعطي بس صورة عنها عن ال
+
+9
+00:00:48,740 --> 00:00:53,720
+functions of viral proteinsCapsid proteins ايش
+
+10
+00:00:53,720 --> 00:00:57,840
+وظيفتها؟ ده ال capsid to protect .. to protect
+
+11
+00:00:57,840 --> 00:01:01,340
+genetic material ممتاز يوه ال .. يوه ال keys تبعها
+
+12
+00:01:01,340 --> 00:01:05,460
+رقم اتنين to mediate attachment يعني هي المسئولة
+
+13
+00:01:05,460 --> 00:01:10,580
+عن الارتباط بالخلايا اللي هيصير لها infection بعد
+
+14
+00:01:10,580 --> 00:01:15,800
+ذلك طبعا هذا الارتباط عبر specific receptors هذا
+
+15
+00:01:15,800 --> 00:01:20,170
+ال interaction اللي بتمثل لبروتيناتمبانها و مبان
+
+16
+00:01:20,170 --> 00:01:23,790
+اللي هو السر مش هو المؤدي لل specificity بمعنى ان
+
+17
+00:01:23,790 --> 00:01:26,150
+this virus will infect this person
+
+18
+00:01:45,280 --> 00:01:50,040
+بقنط horses يبقى فيه نوع من ال specificity اللي
+
+19
+00:01:50,040 --> 00:01:54,100
+بيحددها هو هذا ال protein المسؤول عن ال attachment
+
+20
+00:01:54,100 --> 00:01:57,480
+إذا ال outer membrane protein اللي هو وظيفة رائعة
+
+21
+00:01:57,480 --> 00:02:01,080
+to protect and to mediate attachment they
+
+22
+00:02:01,080 --> 00:02:04,460
+constitute as an important antigensإيش ال
+
+23
+00:02:04,460 --> 00:02:07,580
+important antigens؟ ال antigens هتعلمنا في ال
+
+24
+00:02:07,580 --> 00:02:10,960
+bacteriology و كذلك في ال virology أنه نستخدمها
+
+25
+00:02:10,960 --> 00:02:13,740
+بأكتر من طريقة، واحد في ال vaccine لإنه فيها ال
+
+26
+00:02:13,740 --> 00:02:19,380
+strain variations، صح؟ مثلا ال polio اللي هو
+
+27
+00:02:19,380 --> 00:02:23,980
+الفيروس الشلال، الفيروس ال polio، تمام؟ فيه ال
+
+28
+00:02:23,980 --> 00:02:26,580
+strain واحد و اتنين و تلاتة، إذا بدنا نعمل
+
+29
+00:02:26,580 --> 00:02:31,200
+vaccination بدنا نغطي التلاتةإذا بدنا نميز بين
+
+30
+00:02:31,200 --> 00:02:34,460
+الإصابات عذابنا للفيروس، بدنا نتعرف علي هل هو
+
+31
+00:02:34,460 --> 00:02:38,520
+فيروس واحد ولا اتولدتله؟ طيب ال immune system مش
+
+32
+00:02:38,520 --> 00:02:41,020
+باليستجيب برضه كمان لهذه ال .. ال .. ال .. ال ..
+
+33
+00:02:41,020 --> 00:02:41,500
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+34
+00:02:41,500 --> 00:02:41,580
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+35
+00:02:41,580 --> 00:02:41,860
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+36
+00:02:41,860 --> 00:02:42,700
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+37
+00:02:42,700 --> 00:02:42,840
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+38
+00:02:42,840 --> 00:02:43,700
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+39
+00:02:43,700 --> 00:02:44,620
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+40
+00:02:44,620 --> 00:02:50,140
+ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+41
+00:02:56,630 --> 00:03:00,030
+ضد البروتينات هذه to neutralize ايش يعني
+
+42
+00:03:00,030 --> 00:03:04,110
+neutralize؟ هذا الفيروس هاي ال attachment تبعته،
+
+43
+00:03:04,110 --> 00:03:07,630
+هاي البروتين، ال antibody رطبط فيه، هذا ال
+
+44
+00:03:07,630 --> 00:03:12,110
+antibody عمل blocking للبروتين هذا رطبط منين؟ من
+
+45
+00:03:12,110 --> 00:03:14,910
+ال receptor، وبتالي صار neutralized، اتحيد
+
+46
+00:03:14,910 --> 00:03:19,830
+الفيروس، ايش بنسميها؟ neutralizing antibodies يعني
+
+47
+00:03:19,830 --> 00:03:24,050
+أجسام ولادة عادلة التأثير أو حية للتأثير اللي هو
+
+48
+00:03:24,050 --> 00:03:25,310
+هذا الفيروس
+
+49
+00:03:28,290 --> 00:03:32,950
+الـ internal viral proteins vary depending on the
+
+50
+00:03:32,950 --> 00:03:36,030
+bias، بقولنا إنه الكلام اللي على الأوتر، خد الكلام
+
+51
+00:03:36,030 --> 00:03:40,430
+اللي على العالمين، على ال internal proteins تخيلوا
+
+52
+00:03:40,430 --> 00:03:44,710
+فيروس رايح على خلية بدمانها infection، والماد اللي
+
+53
+00:03:44,710 --> 00:03:48,750
+مراهتيه تبعته negative sense، negative sense mRNA
+
+54
+00:03:50,020 --> 00:03:57,020
+او بوستفسنس بوستفسنس mRNA تمام لكن deployed زي ال
+
+55
+00:03:57,020 --> 00:04:02,760
+HIV وال HIV له ميزة انه بيعمل integration جوا
+
+56
+00:04:02,760 --> 00:04:08,170
+الجينة و تبعناهافيروسينز هدولة راحين على خلية وما
+
+57
+00:04:08,170 --> 00:04:12,490
+لهمش انزيمات .. ما لهمش انزيمات في خلانة تتعامل
+
+58
+00:04:12,490 --> 00:04:16,510
+معاهم كيبنهم يتكاثروا فلازم يحملوا فيروس .. يحملهم
+
+59
+00:04:16,510 --> 00:04:21,070
+انزيماتهم معاهم يبقى بعض ال internal proteins
+
+60
+00:04:21,070 --> 00:04:24,070
+بتكون عبارة عن ايه؟ عبارة عن .. عبارة عن enzymes
+
+61
+00:04:24,070 --> 00:04:27,990
+for the genome replication زي reverse
+
+62
+00:04:27,990 --> 00:04:30,870
+transcriptase اللي بيحملوا ال HI فيه على سبيل
+
+63
+00:04:30,870 --> 00:04:35,400
+المثال وبعض ال .. ال .. الفيروسات الأخرىفي عندي
+
+64
+00:04:35,400 --> 00:04:39,040
+protein يسميه matrix protein فهذا ال matrix
+
+65
+00:04:39,040 --> 00:04:44,400
+protein هو موجود في الفيروسات اللي إلها envelope
+
+66
+00:04:44,400 --> 00:04:51,360
+وهو اللي يعبل المسافة ما بين ال capsid و ما بين ال
+
+67
+00:04:51,360 --> 00:04:54,180
+envelope يعني ال envelope بيكونش معلق في الهواء
+
+68
+00:04:54,180 --> 00:04:57,280
+بيكون بينه و بين ال capsid مجموعة من ال protein
+
+69
+00:04:57,280 --> 00:05:02,800
+يسميها matrix proteinالغريب في الموضوع أنه في بعض
+
+70
+00:05:02,800 --> 00:05:08,040
+الانتيجنات اللي ولدناها في الفيروسات can act as a
+
+71
+00:05:08,040 --> 00:05:11,020
+super antigen فاكرين ال super antigen؟ ال super
+
+72
+00:05:11,020 --> 00:05:15,460
+antigen كان يشغل كيف؟ ب non specific activation لل
+
+73
+00:05:15,460 --> 00:05:20,680
+T lymphocytesمن أجمل ما يكون ان احنا بنقارن ان انا
+
+74
+00:05:20,680 --> 00:05:24,360
+جالين بكتيريا و viruses انه Staphylococcus aureus
+
+75
+00:05:24,360 --> 00:05:28,080
+اذا فاكرين ال toxic shock syndrome toxin فاكرينه؟
+
+76
+00:05:28,080 --> 00:05:32,880
+هذا ال .. ال .. ال toxin كان .. كان عملنا septic
+
+77
+00:05:32,880 --> 00:05:37,080
+shock عن طريق انه عمل non specific activation لل T
+
+78
+00:05:37,080 --> 00:05:42,120
+lymphocyte طيب الفيروسات ليش بتعمل super antigen
+
+79
+00:05:42,120 --> 00:05:48,180
+مثال بقولنا ال Epstein-Barr virusو ال
+
+80
+00:05:48,180 --> 00:05:52,840
+cytomegalovirus و ال retroviruses او ال retrovirus
+
+81
+00:05:52,840 --> 00:05:58,480
+هدى أمثلة على فيروسات بتنتج اللى هوا zovar antigen
+
+82
+00:05:58,480 --> 00:06:02,420
+الاول و التانى اللى هوا ال ابشتين بار فيروس و ال
+
+83
+00:06:02,420 --> 00:06:05,880
+cytomegalovirus كى اللى هم بينتموا الى عائلة اسمها
+
+84
+00:06:06,830 --> 00:06:10,390
+هيربس فايروس يبقى الهيربس فايروس في منها اتنين
+
+85
+00:06:10,390 --> 00:06:12,570
+اللي هم سايتوميجالوفايروس واللي بيشتغلوا
+
+86
+00:06:12,570 --> 00:06:16,630
+بايروفايروس وواحد من عائلة اخرى اسمه رتروفايروس
+
+87
+00:06:16,630 --> 00:06:21,750
+ماوس ماموري ديومارفايروس ايش انها الhypothesis
+
+88
+00:06:21,750 --> 00:06:27,670
+افتراض فرضية فنفترض انه ليش الفيروسات بقولنا the
+
+89
+00:06:27,670 --> 00:06:31,130
+current hypothesis offered to explain why this
+
+90
+00:06:31,130 --> 00:06:33,650
+virus produces a super antigen
+
+91
+00:06:40,600 --> 00:06:48,540
+يعني تخيلوا هو بيهيي الوسط أو البيئة لتكاثره بإنه
+
+92
+00:06:48,540 --> 00:06:52,600
+بيعمل activation لل .. لل .. لل .. لل telemposite
+
+93
+00:06:52,600 --> 00:06:58,060
+بتاعة CD4 عشان هو يلاقي مكان يتكاثر فيه تمام؟ فهو
+
+94
+00:06:58,060 --> 00:07:03,280
+كأنه بينتج culture media لحاله عشان هو يتكاثر في
+
+95
+00:07:03,280 --> 00:07:07,290
+داخلهsome viruses contains regulatory proteins و
+
+96
+00:07:07,290 --> 00:07:09,230
+ال regulatory proteins موجودة احنا نقولها بشوية
+
+97
+00:07:09,230 --> 00:07:13,250
+matrix protein صح؟ بين ال .. ال .. ال capsid و بين
+
+98
+00:07:13,250 --> 00:07:16,930
+ال .. ال .. ال envelope هذي بين المادة الوراثية و
+
+99
+00:07:16,930 --> 00:07:20,350
+بين اللي هو ال .. ال .. ال capsid بين المادة
+
+100
+00:07:20,350 --> 00:07:22,750
+الوراثية و بين ال capsid بسموها بعض الأحيان
+
+101
+00:07:22,750 --> 00:07:27,510
+Tigument ليها .. ليها ده المنطقة، Tigument تمام؟ و
+
+102
+00:07:27,510 --> 00:07:30,450
+located بين ال nickel capsid و ال envelopeهذه
+
+103
+00:07:30,450 --> 00:07:35,210
+البروتينات تحتوي على تنظيم والترجمة والترجمة تحكم
+
+104
+00:07:35,210 --> 00:07:39,290
+أيضًا فيروس أو ترجمة سلوكية يعني البروتينات هذه
+
+105
+00:07:39,290 --> 00:07:45,170
+لها وظائف تنظيمية من حيث الترجمة والترجمة وقد تنظم
+
+106
+00:07:45,170 --> 00:07:51,190
+فيها من خلالها الترجمة وترجمة للفيروسات أو للجسم
+
+107
+00:07:51,190 --> 00:07:56,070
+العادل للجسم العادل نفسهالمثال عليها ال members ل
+
+108
+00:07:56,070 --> 00:07:59,810
+ال herpes virus family زي ال herpes simplex و ال
+
+109
+00:07:59,810 --> 00:08:04,810
+cytomegalovirus بيحتووا على pigment بارزة ايش يعني
+
+110
+00:08:04,810 --> 00:08:08,350
+بارزة بروميلان بارزة and well characterized يعني
+
+111
+00:08:08,350 --> 00:08:11,050
+بنقدر بال electrode microscope نشوفها بشكل مميز
+
+112
+00:08:11,050 --> 00:08:15,810
+الأعلى نيجي لل envelope و احنا تحدثنا عنه صحيح
+
+113
+00:08:15,810 --> 00:08:19,350
+مسبقا في الكلام عامله ببعض الكلمات البسيطة ال
+
+114
+00:08:19,350 --> 00:08:24,850
+envelopeهو membrane يحيط بعض الفيروسات اللي احنا
+
+115
+00:08:24,850 --> 00:08:28,370
+بنسميها envelope viruses، envelope اللي مالهاش
+
+116
+00:08:28,370 --> 00:08:32,730
+سميناها زمان، اما non-envelope او naked او what's
+
+117
+00:08:32,730 --> 00:08:35,490
+in term يعني نوكليو كبسيد، نوكليو كبسيد
+
+118
+00:08:40,320 --> 00:08:42,500
+طب احنا عارف انه فيه advantage و فى just advantage
+
+119
+00:08:42,500 --> 00:08:48,240
+لوجود الemail ال advantage بيسهل عملية ال
+
+120
+00:08:48,240 --> 00:08:52,360
+attachment و ال fusion مع ال email و بالظبط قطرة
+
+121
+00:08:52,360 --> 00:08:55,400
+زيت و قطرة زيت لو حاطيناهم مع بعض ده على طول
+
+122
+00:08:55,400 --> 00:09:00,220
+بيندمج و بيتجاون قطرة واحدة هذه جميزة إيجابية انه
+
+123
+00:09:00,220 --> 00:09:02,860
+بيسهل عملية ال penetration او ال attachment لل
+
+124
+00:09:02,860 --> 00:09:03,180
+penetration
+
+125
+00:09:08,390 --> 00:09:11,870
+بيعمل او بيبقى it confers instability عكس ال
+
+126
+00:09:11,870 --> 00:09:16,780
+stability وبالتالي بيلاقي انههذه الفيروسات هذه
+
+127
+00:09:16,780 --> 00:09:22,740
+الفيروسات نجدها بتنتقل عبر ال intimate contact اما
+
+128
+00:09:22,740 --> 00:09:26,580
+sexual contact او blood transfusion و بعض الأحيان
+
+129
+00:09:26,580 --> 00:09:29,740
+عبر ال respiratory tract عبر ال respiratory tract
+
+130
+00:09:29,740 --> 00:09:33,320
+لكن ولا واحد من ال envelope viruses بينتقل عزيزي
+
+131
+00:09:33,320 --> 00:09:36,860
+مثال عبر الفكهة ال oral عبر الفكهة ال oral لأنه
+
+132
+00:09:36,860 --> 00:09:39,580
+هيمر عبر المائدة و المائدة فيها حالة دائمة
+
+133
+00:09:39,580 --> 00:09:43,000
+destruction للممبرينت وبالتاليالميزة اللى كانت
+
+134
+00:09:43,000 --> 00:09:45,700
+موجودة فيه ان الممبران بيسهلوا عملية ال attachment
+
+135
+00:09:45,700 --> 00:09:49,180
+إذا راح الممبران مش هيقدر يعمل attachment انت هنا
+
+136
+00:09:49,180 --> 00:09:53,220
+من الجثة يبجأ ال viral envelope بدالي فهم أنه إله
+
+137
+00:09:53,220 --> 00:09:56,420
+هذا ال advantage و إله ال disadvantage اللى موجود
+
+138
+00:09:56,750 --> 00:10:00,850
+طيب في كمان نقطة انه بيحتوي على proteins في ال
+
+139
+00:10:00,850 --> 00:10:03,590
+membrane هذه ال proteins اللي بيسميها ال
+
+140
+00:10:03,590 --> 00:10:07,230
+glycoproteins او ال GP هذي ال GP من ورا .. من ورا
+
+141
+00:10:07,230 --> 00:10:10,650
+.. من ورا المخودة مش مورودة في خلايا هذه الفيروس
+
+142
+00:10:10,650 --> 00:10:14,970
+بيصنعها هو داخل الخلية هو داخل الخلية و بيغرسها في
+
+143
+00:10:14,970 --> 00:10:18,290
+ال cell membrane و هو طالع بياخد membrane صح؟
+
+144
+00:10:18,290 --> 00:10:21,550
+بياخد membrane فبياخد ال membrane المغروس في اللي
+
+145
+00:10:21,550 --> 00:10:21,770
+هو
+
+146
+00:10:28,480 --> 00:10:32,140
+هذه ال spikes إليها وظائف مش مجرد أنه والله spikes
+
+147
+00:10:32,140 --> 00:10:34,920
+منظر يعني عاملينها لل .. للويروس، لأ إليها وظائف
+
+148
+00:10:34,920 --> 00:10:39,000
+من ضمنها .. من ضمنها .. من ضمنها إنها receptor
+
+149
+00:10:39,000 --> 00:10:44,460
+لخلايا أخرى، هي receptor لخلايا أخرىطب في هذه
+
+150
+00:10:44,460 --> 00:10:48,260
+اللحظة مضطرين ان ننقل على نشوف عملية replication
+
+151
+00:10:48,260 --> 00:10:52,840
+الشكل اللي قدامنا او اللوحة اللي قدامنا فيها تلت
+
+152
+00:10:52,840 --> 00:10:55,580
+أجزاء رئيسية تلت أعمدة العمود الأول attachment and
+
+153
+00:10:55,580 --> 00:10:58,360
+penetration العمود التاني synthesis و العمود
+
+154
+00:10:58,360 --> 00:11:02,900
+التالت assembly and release لان انا هتكلم عن ال
+
+155
+00:11:02,900 --> 00:11:06,420
+attachment و ال penetration نشوف هي فيروس هذا ليس
+
+156
+00:11:06,420 --> 00:11:11,900
+له envelope non envelope يعني هذا naked virus عمل
+
+157
+00:11:11,900 --> 00:11:16,560
+attachment على ال membraneوعمل emptying لل content
+
+158
+00:11:16,560 --> 00:11:19,520
+هو لسه برا زى ال bacteriophage فى ال bacteriophage
+
+159
+00:11:19,520 --> 00:11:25,140
+هذا نموذج النموذج التانى اللى احنا بدنا نشوفه اللى
+
+160
+00:11:25,140 --> 00:11:29,400
+هو ال membrane fusion لمن؟ لل invert هذا invert هى
+
+161
+00:11:29,400 --> 00:11:37,320
+receptors شوفوا الآن عملية ال fusion ومن ثم
+
+162
+00:11:37,320 --> 00:11:41,460
+emptying لمن؟ لل virus اللى هو ال nucleocapsid إلى
+
+163
+00:11:41,460 --> 00:11:47,370
+داخل الخليةالتالتة المسمّة تبعها الصحيح يعني لا
+
+164
+00:11:47,370 --> 00:11:51,810
+يعبّل عن ما نقوله كلمة phagocytosis عمالية معروفة
+
+165
+00:11:51,810 --> 00:11:56,390
+لكن هذه تشبهها نسميها endocytosis مش phagocytosis
+
+166
+00:11:56,390 --> 00:12:02,410
+أو penocytosis الهم إنه فيه entry مش خروج للأعلى
+
+167
+00:12:02,410 --> 00:12:08,030
+invagination للأسفل تشوفوا الأعلى اللقطة هذه إنه
+
+168
+00:12:08,030 --> 00:12:10,650
+الفيروس دخل إلى الداخل وتكون في physical زي
+
+169
+00:12:10,650 --> 00:12:16,010
+الفاجوزوم زي الفاجوزومهي طرق اللي هو إيه ال
+
+170
+00:12:16,010 --> 00:12:19,450
+attachment و ال entry للفيروس طبعا إذا بتلاحظوا
+
+171
+00:12:19,450 --> 00:12:25,750
+هنا في جليكو بروتين صح هذه الجليكو بروتين مابتكونش
+
+172
+00:12:25,750 --> 00:12:30,050
+موجودة على الخلية هذه الآن بالمنظر اللي شايفينه
+
+173
+00:12:30,050 --> 00:12:33,310
+قدامك وهذا هي أيضا عبارة عن receptors للفيروس هي
+
+174
+00:12:33,310 --> 00:12:36,950
+receptors للفيروس لو شفنا ال .. ال .. ال release
+
+175
+00:12:36,950 --> 00:12:41,670
+نشوف ال release مباشرة تمام
+
+176
+00:12:44,030 --> 00:12:49,590
+بنقفز على ال synthesis بعد ما نشوفه بيشتغل
+
+177
+00:12:49,590 --> 00:12:56,970
+هيك ال naked virus بيتغير لما تعمل lysis لخلية و
+
+178
+00:12:56,970 --> 00:12:59,590
+بتطلع بتدمرها تعملها destruction متذكروا كلمة
+
+179
+00:12:59,590 --> 00:13:03,750
+lysis؟ خلية دمرت من ضمن أنسجة هينتقل للفيروس
+
+180
+00:13:03,750 --> 00:13:06,350
+لخلايا أخرى و يدمرها فهيصير في النتيجة اللي فيها
+
+181
+00:13:06,350 --> 00:13:09,230
+انها destruction حد بيسميها cytopathic effect
+
+182
+00:13:09,230 --> 00:13:13,710
+نعرفها كويس للمحاضرة الجايةيبقى صار في end release
+
+183
+00:13:13,710 --> 00:13:16,370
+بالdestruction الان نجي لل envelope viruses ال
+
+184
+00:13:16,370 --> 00:13:20,790
+envelope viruses اللي لها ذكية قد تتكاثر و تبقى في
+
+185
+00:13:20,790 --> 00:13:24,470
+الخلية و تخرج من الخلية دول تدمر هذه الخلية عن
+
+186
+00:13:24,470 --> 00:13:26,810
+طريق هاي ال proteins اللي بتصنعها ال glycoprotein
+
+187
+00:13:26,810 --> 00:13:34,870
+تم لها insertion في ال cell membrane و
+
+188
+00:13:34,870 --> 00:13:39,210
+من ثم الفيروس بيهو خارج و بياخد يزور من ال
+
+189
+00:13:39,210 --> 00:13:43,710
+membrane زي ما انتوا زي ما انا بدي اتحركو بياخد
+
+190
+00:13:43,710 --> 00:13:47,310
+جزء و بيسموه العملية body كأنه تبرع و كأنه أخد
+
+191
+00:13:47,310 --> 00:13:52,950
+قطعة و طلع فيها فلكن ماصارش ايه؟ destruction ل
+
+192
+00:13:52,950 --> 00:14:01,310
+الخلايا هاي
+
+193
+00:14:01,310 --> 00:14:06,410
+ال glycoprotein زي ما حكينا و بترسلوها ال GPطبعا
+
+194
+00:14:06,410 --> 00:14:09,150
+تختلف من virus ل virus تركيبها و طبيعتها وبتالي هي
+
+195
+00:14:09,150 --> 00:14:13,070
+محدد للانتجينات لاحظوش صار destruction ماصارش
+
+196
+00:14:13,070 --> 00:14:26,470
+destruction ل الخلاق تكلمنا
+
+197
+00:14:26,470 --> 00:14:28,190
+عن يزويتين في ال envelope
+
+198
+00:14:31,420 --> 00:14:36,480
+أو سلبية واتكلمنا عن مكونات ال spikes اللي هي ال
+
+199
+00:14:36,480 --> 00:14:42,040
+glycoproteins اللي موجودة في ال embryo و حكينا عن
+
+200
+00:14:42,040 --> 00:14:43,920
+ال instability اللي هي ال keyword في اللوحة اللي
+
+201
+00:14:43,920 --> 00:14:48,620
+شايفينها ال instability من وين جاية كان مرة بأن ال
+
+202
+00:14:48,620 --> 00:14:51,160
+membrane very sensitive بصيرله destruction و
+
+203
+00:14:51,160 --> 00:14:54,700
+بصيرله destructionطبعا ال destruction هلأ بيصير
+
+204
+00:14:54,700 --> 00:14:59,840
+بأي من المراكبات السهلة و من ضمنها الجفاف .. من
+
+205
+00:14:59,840 --> 00:15:03,460
+ضمنها الجفاف يعني لو الفيروس رأى على براية ال
+
+206
+00:15:03,460 --> 00:15:09,040
+clinics تمام على طول بيصير فيه dryness وبالتالي
+
+207
+00:15:09,040 --> 00:15:14,140
+بيجف و بتدمر ال cell membrane ليه؟ عشان أقولنا ال
+
+208
+00:15:14,140 --> 00:15:17,620
+transmission تبعه بيكون فقط بال intimate contact
+
+209
+00:15:17,620 --> 00:15:19,880
+blood transfusion أو sexual contact أو بعض الأحيان
+
+210
+00:15:19,880 --> 00:15:24,810
+الrespiratory بقرعه في ال environmentأقل من بقاء
+
+211
+00:15:24,810 --> 00:15:29,290
+اللي هو ال naked virus تذكروا فيروس الرشح اللي
+
+212
+00:15:29,290 --> 00:15:32,530
+بينتقل عبر ال respiratory تمام هو naked virus
+
+213
+00:15:32,530 --> 00:15:39,050
+naked virus وقد يعني انه صارت بالرشح صارت بالرشح
+
+214
+00:15:39,050 --> 00:15:43,670
+مع انه احنا ماحتكينا بمرضى وماجفنا مرضى اصلا عندهم
+
+215
+00:15:43,670 --> 00:15:50,720
+common cold والسبب انه الفيروس فيولو لمسنا
+
+216
+00:15:50,720 --> 00:15:54,120
+بأصابعنا أو دخّان أصابعنا في الconductiva أو بوصل
+
+217
+00:15:54,120 --> 00:16:08,120
+الأنف أو الفن بطريقة ما ممكن ينتقل لكل إنسان هنا
+
+218
+00:16:08,120 --> 00:16:10,880
+نفس المعلومات اللي حكيناها أنه ال .. ال antigenات
+
+219
+00:16:10,880 --> 00:16:13,020
+اللي مولدة على السطح هي اللي بتشكل ال stereotypes
+
+220
+00:16:13,020 --> 00:16:16,280
+هنا كلام مكرر من الكلام اللي حكيناه بالأولنجي إلى
+
+221
+00:16:16,280 --> 00:16:20,000
+الجزء الأخير من الموضوع Atypical viruses like
+
+222
+00:16:20,000 --> 00:16:23,160
+Asian defective viruses, pseudovirions, virioids
+
+223
+00:16:23,160 --> 00:16:28,020
+and priors طبعا على الـ defective viruses نبدأ
+
+224
+00:16:28,020 --> 00:16:31,320
+بالـ defective viruses الـ defective viruses هي
+
+225
+00:16:31,320 --> 00:16:36,960
+بالاصطلاح العامي فيروسات يعني خربانة خربانة بمعنى
+
+226
+00:16:36,960 --> 00:16:42,140
+أنه مابتقدرش تتكاثر إلا في وجود ما يسمى بالهيلفر
+
+227
+00:16:42,140 --> 00:16:46,630
+فيروسهنا بتطلع عليها من زاوية تانية، زاوية فيروس
+
+228
+00:16:46,630 --> 00:16:50,790
+بسبب برض إنه معروف عنه defective اسمه Hepatitis D
+
+229
+00:16:50,790 --> 00:16:56,810
+Hepatitis D بتاعه بو A و B و C و فيها إنه D، D هذا
+
+230
+00:16:56,810 --> 00:17:00,370
+مستحيل يشوفه عند بني آدم إلا لازم يكون معاه بيه،
+
+231
+00:17:00,370 --> 00:17:04,450
+لكن مش بالضرورة إنه إذا كان معاه بيه يكون معاه دي
+
+232
+00:17:05,050 --> 00:17:10,150
+يبقى وجود D مرتبط بمين؟ بالعكس مش صحيص أنه ال B مش
+
+233
+00:17:10,150 --> 00:17:13,410
+مرتبط بيه، يعني لو انا اليوم من الأيام فحصل واحد
+
+234
+00:17:13,410 --> 00:17:17,050
+عن D و لقيته positive، ما انت تحكي عنه؟ أندوبي،
+
+235
+00:17:17,050 --> 00:17:20,830
+without B virus they cannot replicate، they cannot
+
+236
+00:17:20,830 --> 00:17:25,110
+replicate طيب هذه نقطة مهمة و كويسة في الطب أنه
+
+237
+00:17:25,110 --> 00:17:28,690
+نعرف أن فيه فيروسات معتمدة على فيروسات أخرىالنقطة
+
+238
+00:17:28,690 --> 00:17:32,390
+التانية بدنا نستخدمها في تفسير لماذا نشفى من
+
+239
+00:17:32,390 --> 00:17:37,710
+الفيروسات تلقائيا بدون علاج بعض الأحيان أو بكثير
+
+240
+00:17:37,710 --> 00:17:41,150
+من الأحيان أو بكثير من الأمر الفيروسي لو قلنالكوا
+
+241
+00:17:41,150 --> 00:17:45,090
+أن ال defective virus هو عملية روتينية في التصنيع
+
+242
+00:17:45,090 --> 00:17:49,950
+العادي للفيروسات يعني فيروس دخل خلية واتكاتر جوامش
+
+243
+00:17:49,950 --> 00:17:54,010
+احنا ضمن التكارثة فيها اسم assembly، تجمية، فبقول
+
+244
+00:17:54,010 --> 00:17:59,730
+إن عند التجمية قد يحصل .. قد يحصل خلل، فجزء من ال
+
+245
+00:17:59,730 --> 00:18:03,570
+viral genome يدخل و جزء مايدخلش، وبالتالي الفيروس
+
+246
+00:18:03,570 --> 00:18:08,960
+بيكون إيش الآن؟ ناقص، defectiveقد يصل عدد الـ
+
+247
+00:18:08,960 --> 00:18:14,720
+defective بالمقارنة مع الصحيح 100 ل 1 تخيلوا واحد
+
+248
+00:18:14,720 --> 00:18:20,200
+بس الصحيح و 100 قد يصل ممكن 50 50 ممكن 70 30 لكن
+
+249
+00:18:20,200 --> 00:18:24,000
+في الآخر في نسبة معينة .. نسبة معينة من ال
+
+250
+00:18:24,000 --> 00:18:27,240
+defective virus معروف انها بتنتج في كل cycle
+
+251
+00:18:27,240 --> 00:18:32,400
+الفيروس طب نتخيل مع بعض دخل 100 فيروس عمل
+
+252
+00:18:32,400 --> 00:18:37,370
+infection ل 100 خليةمليار مليار مليار مليار مليار
+
+253
+00:18:37,370 --> 00:18:38,210
+مليار مليار مليار مليار مليار مليار مليار مليار
+
+254
+00:18:38,210 --> 00:18:52,930
+مليار مليار مليار
+
+255
+00:19:04,590 --> 00:19:08,010
+الجهاز المنعها، إذا ال defective virus استخدم في
+
+256
+00:19:08,010 --> 00:19:11,410
+تفسير لماذا هناك شفاء تلقائي لمن بعض الأمراض
+
+257
+00:19:11,410 --> 00:19:16,150
+الفيروسية نجد التاني اللي هو ال pseudo virus،
+
+258
+00:19:16,150 --> 00:19:22,910
+pseudo معناه كاذب هو شكله virus لكن من جوا جلبه مش
+
+259
+00:19:22,910 --> 00:19:28,520
+virus، جلبه hostماخد DNA من اللي هو ال cell اللي
+
+260
+00:19:28,520 --> 00:19:31,340
+شفناها لما دراسنا في ال bacteriophage لما اتكلمنا
+
+261
+00:19:31,340 --> 00:19:34,520
+عن ال generalized transduction و ان خلال التعبية
+
+262
+00:19:34,520 --> 00:19:39,700
+قد يدخل إلى داخله بكتيريا دياني صح؟ و بعدين ينتقل
+
+263
+00:19:39,700 --> 00:19:42,560
+هذا بيسميه pseudo variant و هذا okay بيعمل
+
+264
+00:19:42,560 --> 00:19:46,340
+infection لكن لن يحدث تكاثر، مافيش تكاثر، بيصيب
+
+265
+00:19:46,340 --> 00:19:50,320
+خلية و يدخل داخلها لكن مافيش تكاثر يعني they can
+
+266
+00:19:50,320 --> 00:19:53,560
+infect cell but they do not replication تمام؟
+
+267
+00:19:53,560 --> 00:19:54,060
+مافيش replication
+
+268
+00:20:03,050 --> 00:20:07,430
+هو .. هو عبارة عن .. شوفنا العجاب في ال micro
+
+269
+00:20:07,430 --> 00:20:10,870
+biology كنا نتكلم عن بكتيريا و بعدها نتكلم عن
+
+270
+00:20:10,870 --> 00:20:14,110
+كلاميديا و الكيتسيا اللي هو ال obligated cell و
+
+271
+00:20:14,110 --> 00:20:16,350
+بعدها انتقلنا للفيروسات و بيقولنا الفيروسات حاجة
+
+272
+00:20:16,350 --> 00:20:19,630
+غريبة و بعدها انتقلنا من ديوال الفيروسات الى
+
+273
+00:20:19,630 --> 00:20:23,730
+الفيرويدس الفيرويدس عبارة عن مادة وراثية مكونة من
+
+274
+00:20:23,730 --> 00:20:29,590
+mRNA mRNA فقط مافيش لا بروتين ولا غيره ولا كبسيدو
+
+275
+00:20:29,590 --> 00:20:33,610
+ولا اي حاجةالميزة الوحيدة أن هذا ال mRNA إذا دخل
+
+276
+00:20:33,610 --> 00:20:38,450
+خلية معينة من ال host .. suitable host بيعمل
+
+277
+00:20:38,450 --> 00:20:41,810
+replication و هم يمكنوا يعملوا infection على الرغم
+
+278
+00:20:41,810 --> 00:20:46,890
+من أن العلماء لم يجدوا في ال mRNA هذا أي code لأي
+
+279
+00:20:46,890 --> 00:20:52,870
+protein، لحد لأن مافيش شنة فهل لفبيرة تكوين المادة
+
+280
+00:20:52,870 --> 00:20:58,210
+الوراثيةلأنه فيها homology كتير باللاجئ طبعا هي
+
+281
+00:20:58,210 --> 00:21:01,170
+circle لو أخدوا بالكم لكن لأنه فيها homology كتير
+
+282
+00:21:01,170 --> 00:21:05,190
+باللاجئ فيها صار loops ايش يعني loops؟ يعني
+
+283
+00:21:05,190 --> 00:21:09,130
+اتشربجوا مع بعض لإن هو هذا مرتبط مع هذا فصار عندي
+
+284
+00:21:09,130 --> 00:21:14,810
+loops يصيب النباتات ويسبب بعض الأمراض للنباتات لكن
+
+285
+00:21:14,810 --> 00:21:19,150
+لم يثبت أنه ال viroids بتسبب للإنسان أو الحيوان أي
+
+286
+00:21:19,150 --> 00:21:27,170
+من العضار defective, pseudo و viroidالرابع الأهم
+
+287
+00:21:27,170 --> 00:21:30,310
+على الإطلاق و جيد من انا ابدأ فيه بدأ ادخل فيه
+
+288
+00:21:30,310 --> 00:21:38,110
+اعمل عرض برضه صغير اوضح ايش يعني شخص بتناول غذاء
+
+289
+00:21:38,110 --> 00:21:42,170
+معين محتوى على ال بريون ال بريون عبارة عن مختصار
+
+290
+00:21:42,170 --> 00:21:45,810
+لكلمة protein-infectious هذا الغريب في الموضوع
+
+291
+00:21:45,810 --> 00:21:50,390
+protein-infectious بريون صارلا يحتوي على مادة
+
+292
+00:21:50,390 --> 00:21:54,870
+ويراتية ولا بيحتوي على كبسيد ولا اي .. فقط بروتين
+
+293
+00:21:54,870 --> 00:21:59,310
+من نوع معين و هنتفاجأ ان هذا البروتين هو البروتين
+
+294
+00:21:59,310 --> 00:22:05,210
+احنا بننتجه .. احنا بننتجه بشكل طبيعيلكن انتجه
+
+295
+00:22:05,210 --> 00:22:09,810
+بشكل X يتحول ل Y تختلف تماما بيصير abnormal
+
+296
+00:22:09,810 --> 00:22:12,750
+والمشكلة زمان احنا كنا نقول ان اللي بيمشي مع
+
+297
+00:22:12,750 --> 00:22:15,770
+الفاسد ايش بيصير معها بيصير معها الفاسد وهو هذا
+
+298
+00:22:15,770 --> 00:22:19,710
+حقيقة صح ولا الفاسد بيحاول يفسد الناس اللي حاوله
+
+299
+00:22:19,710 --> 00:22:24,770
+وهذا بيشتغل بنفس الطريقة فسد هو تغير من الف إلى ر
+
+300
+00:22:24,770 --> 00:22:30,710
+من alpha confirmation إلى beta shaded او beta
+
+301
+00:22:30,710 --> 00:22:35,890
+bladed shadedأتحول فكل ما بيمس normal بيحول لأوضه
+
+302
+00:22:35,890 --> 00:22:39,730
+normal فالتكاثر من وين جاي من برا أو تكاثر بال
+
+303
+00:22:39,730 --> 00:22:42,170
+simple binary vision أو بإنه بيدخل دلوقتي الخلايا
+
+304
+00:22:42,170 --> 00:22:47,850
+و بيتكاثر لأ فقط بيصير و كأنه جالب إذا مسك ال
+
+305
+00:22:47,850 --> 00:22:52,330
+remote control هذا بيجلبه تهمهم صار من النوع
+
+306
+00:22:52,330 --> 00:22:58,670
+الخبيث لذلك الاسم بتعرفش عليه أساسالأن هو أصلا
+
+307
+00:22:58,670 --> 00:23:02,790
+إبنه، من الجسم، من الجسم فبالتالي بيخرب الدماغ،
+
+308
+00:23:02,790 --> 00:23:07,270
+بصير فيه زي الإسفنجة، مخزج، تمام؟ ثقوب، أي شيء
+
+309
+00:23:07,270 --> 00:23:12,110
+بيسموه spongiform encephalopathy مرض في الدماغ
+
+310
+00:23:12,110 --> 00:23:15,970
+شكله .. شكله إسفنجي لأن الدماغ بعد تشريها بيكون
+
+311
+00:23:15,970 --> 00:23:24,010
+بهذا الشكل خلينا نطلع عليه هي
+
+312
+00:23:24,010 --> 00:23:29,940
+ال normal bryons اللي موجودةهي ال abnormal، الآن
+
+313
+00:23:29,940 --> 00:23:32,520
+ال abnormal هي من الاسم غير من الخارج احنا، مع
+
+314
+00:23:32,520 --> 00:23:34,720
+التعقيدات اللي بيسموها transmissible، ايش هى
+
+315
+00:23:34,720 --> 00:23:39,360
+transmissible؟ قابل للانتقال، شوفوا الآن، مجرد انه
+
+316
+00:23:39,360 --> 00:23:43,200
+اتلنس مع ال normal صار abnormal، يبقى التكاثر تبعه
+
+317
+00:23:43,200 --> 00:23:47,580
+معتمد على موجوده، هحكي بعض الحقائق تثبت الكلام هذا
+
+318
+00:23:51,980 --> 00:23:55,340
+طبعا حد هقولي إيش ال bryons؟ ال bryons عبارة عن
+
+319
+00:23:55,340 --> 00:23:58,760
+receptors موجودة في الخلايا تبعتنا لكن الوظيفة
+
+320
+00:23:58,760 --> 00:24:05,040
+تبعتهم لحد الآن علامة استفهام ليش علامة استفهام؟
+
+321
+00:24:05,040 --> 00:24:08,140
+البعض طبعا بيقول إن هي receptor إيه اللي علاقة بال
+
+322
+00:24:08,140 --> 00:24:11,480
+transduction عارفين شو هي transduction؟ إيش هي
+
+323
+00:24:11,480 --> 00:24:13,620
+transduction؟
+
+324
+00:24:27,430 --> 00:24:32,270
+أي خلية نفطرنها خاملة في عليها receptor عليها
+
+325
+00:24:32,270 --> 00:24:37,070
+receptors تيرة وانا ارجع انك تتخيل الخلية عبارة عن
+
+326
+00:24:37,070 --> 00:24:42,470
+كائن فضائي .. كائن فضائي مليء بالشعر الدقيق التويل
+
+327
+00:24:42,470 --> 00:24:46,470
+الجسير الأحمر الأصفر الأحمر اللوى غنية جدا بال
+
+328
+00:24:46,470 --> 00:24:51,270
+receptors ما ننظرش إليها إنها عبارة عن صندوق أملاس
+
+329
+00:24:51,270 --> 00:24:56,650
+لأ الخلية أعقد من ذلك بكثيرفيها Receptor أو
+
+330
+00:24:56,650 --> 00:25:01,190
+Receptors عفواً إيجا Molecule ويرتبط بال Receptor
+
+331
+00:25:01,190 --> 00:25:07,010
+مجرد أن ال Molecule يرتبط بال Receptor ال Receptor
+
+332
+00:25:07,010 --> 00:25:09,550
+بيصير له activation بيعمل activation لمركبات جوا
+
+333
+00:25:09,550 --> 00:25:12,950
+المركبات اللي جوا بتنقل إشارة إشارة إشارة هذا اللي
+
+334
+00:25:12,950 --> 00:25:16,170
+عايز نسميه إحنا إيه Signal Signal إشارة Signal
+
+335
+00:25:16,170 --> 00:25:20,790
+Transduction فيعتقد إن من ال Molecules المهمة في
+
+336
+00:25:20,790 --> 00:25:23,510
+مين في ال Signal Transduction لكن
+
+337
+00:25:26,550 --> 00:25:30,810
+لكن بنطلق مصطلح اليوم جديد عليكم اسمه knockout
+
+338
+00:25:30,810 --> 00:25:36,050
+mice ايش يعني knockout؟ knockout mice هي فقران
+
+339
+00:25:36,050 --> 00:25:41,710
+تنتج بحيث انه يكون فيها خلل في جين محدد فلما أقول
+
+340
+00:25:41,710 --> 00:25:49,130
+Bryans knockout mice يعني فقران فيها خلل في الجين
+
+341
+00:25:49,130 --> 00:25:54,250
+تبع مين؟ تبع ال Bryansلما طعمناها .. اعطناها يعني
+
+342
+00:25:54,250 --> 00:25:58,290
+.. او حقنناها بال up normal brain ما صار عندها
+
+343
+00:25:58,290 --> 00:26:02,050
+المرض لأن
+
+344
+00:26:02,050 --> 00:26:04,850
+مافيش فيها ال gene وبالتالي مش هيتكار أثر لأن
+
+345
+00:26:04,850 --> 00:26:08,690
+مافيش فيها ال protein صح ال normal protein المشكلة
+
+346
+00:26:08,690 --> 00:26:14,270
+أن الفقران اللي مافيش فيها ال brains طبعا برضه عشت
+
+347
+00:26:14,270 --> 00:26:18,760
+normalبمعنى انه ماتأثرتش الوظيفة تبعته لذلك بنقول
+
+348
+00:26:18,760 --> 00:26:23,140
+ان الوظيفة تبعت Libraions is still unknown او
+
+349
+00:26:23,140 --> 00:26:29,340
+unclear لكن الدراسات أثبتت انه مستوى Libraion
+
+350
+00:26:29,340 --> 00:26:34,680
+كقياس في ال infected و ال non-infected هو واحد و
+
+351
+00:26:34,680 --> 00:26:38,760
+الدراسات أثبتت ان ال mRNA تبع الجين تبع Libraions
+
+352
+00:26:38,760 --> 00:26:42,440
+في ال infected و ال non-infected برضه واحد فايش
+
+353
+00:26:42,440 --> 00:26:47,390
+ضرر نفسر هناالتفسير كان تالي إن التغيير هو post
+
+354
+00:26:47,390 --> 00:26:51,970
+-translational إيش يعني post-translational يعني
+
+355
+00:26:51,970 --> 00:26:55,830
+بعد ال protein level اللي هو اتبعتنا اللي قولنا
+
+356
+00:26:55,830 --> 00:27:01,610
+Alpha تحول إلى Beta بليتشيت وبالتالي بالفعل صار هو
+
+357
+00:27:01,610 --> 00:27:06,370
+أكبر إذا في مجموعة من هذه الحقائق جلتلنا إن ال
+
+358
+00:27:06,370 --> 00:27:10,690
+bryons عبارة عن بروتينات normal موجودة في خليانة
+
+359
+00:27:10,900 --> 00:27:14,180
+لكن الـ abnormal form هو اللي بيخليها abnormal
+
+360
+00:27:14,180 --> 00:27:20,580
+وكونها self فالبسم بيخرب بيتدمر الدماغ دون أن يكون
+
+361
+00:27:20,580 --> 00:27:23,960
+هناك immune response دون أن يكون هناك inflammatory
+
+362
+00:27:23,960 --> 00:27:27,800
+response يعني لا فيه inflammation لا فيه اللي هو
+
+363
+00:27:27,800 --> 00:27:32,140
+ال .. ال .. ال .. أحد النقاط المهمة اللي بتتكلم
+
+364
+00:27:32,140 --> 00:27:36,700
+فيها أن ال bryons are more resistant than viruses
+
+365
+00:27:36,700 --> 00:27:39,880
+to inactivation by heat or by ultraviolet
+
+366
+00:27:39,880 --> 00:27:44,340
+radiationتخيلين؟ يعني الأن صار عملية تعقيم
+
+367
+00:27:44,340 --> 00:27:49,460
+والتطهير أصعب من دي قبل لإنه ال UV كانت ممتازة في
+
+368
+00:27:49,460 --> 00:27:53,340
+قتل الفيروسات والبكتيريا لكن الأن هي مش ممتازة في
+
+369
+00:27:53,340 --> 00:27:57,920
+قتل ال primers حتى ال formaldehyde اللي لما
+
+370
+00:27:57,920 --> 00:28:01,260
+اتكلمنا عنه ال antiseptic disinfectant كنا عنه من
+
+371
+00:28:01,260 --> 00:28:02,420
+أعظم ومن أقصر
+
+372
+00:28:05,730 --> 00:28:09,370
+لكن هناك مركبات هو الحقيقة sensitive لها زى مثلا
+
+373
+00:28:09,370 --> 00:28:13,850
+اللى هو ال hypochlorite الوليوكلوريا والانيواتش
+
+374
+00:28:13,850 --> 00:28:19,450
+والاوتوكليفن كفين لقتل الميكروبطالي المواد اللى
+
+375
+00:28:19,450 --> 00:28:21,610
+خايفين انا اعمل اوتوكليفن ممكن اعاقنها بمين
+
+376
+00:28:21,610 --> 00:28:27,060
+بالhypochlorite اه بدى بس اذكر بعض الأمثلةال
+
+377
+00:28:27,060 --> 00:28:30,580
+disease بشكل عام سمّنها transmissible spongiform
+
+378
+00:28:30,580 --> 00:28:33,900
+encephalopathy كلمة transmissible يعني قبل أن
+
+379
+00:28:33,900 --> 00:28:37,260
+تتقال، spongiform قلنا شكله شكل الدماغ زي
+
+380
+00:28:37,260 --> 00:28:40,420
+الأسفنجة، الدماغ بيكون encephalopathy لأنه في
+
+381
+00:28:40,420 --> 00:28:47,460
+الدماغ أشهر مثلين واحد بشري اسمه Creutzfeld Jakob
+
+382
+00:28:47,460 --> 00:28:53,530
+DiseaseKreutzfeldt-Jakob Disease وهو يماثل مرض
+
+383
+00:28:53,530 --> 00:28:57,190
+جنون البقر، مرض جنون البقر، يعني هذا اسمه جنون
+
+384
+00:28:57,190 --> 00:29:00,730
+البشر، جنون البشر اسمه Jakob أو Kreutzfeldt-Jakob
+
+385
+00:29:00,730 --> 00:29:04,690
+Disease وفي مرض تاني في الخراف بيسموه Scrabie، وهو
+
+386
+00:29:04,690 --> 00:29:10,850
+الأول، والأشهر على الإطلاق Scrabie، وبالتالي حتى
+
+387
+00:29:10,850 --> 00:29:15,170
+المسماء تبع ال .. ال .. ال .. بيسموها Bryons P-R-B
+
+388
+00:29:15,170 --> 00:29:18,010
+-S-C، S-C من Scrabie، S-C من Scrabie
+
+389
+00:29:21,680 --> 00:29:25,480
+النقاط المهمة كمان كونه لا يحتوي على مادة وراثية
+
+390
+00:29:25,480 --> 00:29:28,080
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+391
+00:29:28,080 --> 00:29:28,700
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+392
+00:29:28,700 --> 00:29:30,400
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+393
+00:29:30,400 --> 00:29:31,880
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+394
+00:29:31,880 --> 00:29:33,580
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+395
+00:29:33,580 --> 00:29:34,740
+ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال
+
+396
+00:29:34,740 --> 00:29:34,940
+ال ال ال ال ال ال ال ال ال ال ال
+
+397
+00:29:42,670 --> 00:29:46,110
+الـ appearance in the electron microscope الـ
+
+398
+00:29:46,110 --> 00:29:49,910
+prions appear filamentous بينما الفيروس اما تكون
+
+399
+00:29:49,910 --> 00:29:53,050
+ايكوزاهيدرال او الهيليكل اللي حكينا عنها او ال
+
+400
+00:29:53,050 --> 00:29:56,870
+complex confirmation الجنود الأخيرة اتفقنا عليها
+
+401
+00:29:56,870 --> 00:30:01,710
+انه no immunity is induced و no inflammation there
+
+402
+00:30:01,710 --> 00:30:06,410
+الحالة
+
+403
+00:30:06,410 --> 00:30:12,770
+ذراعها من 27 ل 30كيلو دالتون بيسموه كيلو دالتون،
+
+404
+00:30:12,770 --> 00:30:16,130
+هذا وزن، وزن ال .. ال .. ال protein نفسه، nuclear
+
+405
+00:30:16,130 --> 00:30:19,910
+weight وهي أبدا ال glycoprotein زي ما حكينا بتكون
+
+406
+00:30:19,910 --> 00:30:25,090
+encoded by our own genes، في أن الكلام اللي حكيناه
+
+407
+00:30:25,090 --> 00:30:27,970
+قبل شوية المتعلق والباص transitional ..
+
+408
+00:30:27,970 --> 00:30:31,430
+translational modification، انه تحويل بتنبع ال
+
+409
+00:30:31,430 --> 00:30:37,060
+translationهي Alpha و هي Beta تتغير تمام؟ و اللي
+
+410
+00:30:37,060 --> 00:30:39,700
+اتشرحه اللي شرحته انا على ال knockout mice اللي هو
+
+411
+00:30:39,700 --> 00:30:44,540
+ان ال knockout mice ماتأثرتش لما حفنها بال scrubby
+
+412
+00:30:44,540 --> 00:30:49,640
+برايونز لإنه مافيش فيها normal protein
+
+413
+00:30:51,100 --> 00:30:54,020
+زي ما حكيتها ال function كمان مش واضحة فيها اعتقاد
+
+414
+00:30:54,020 --> 00:30:57,740
+ان هي علاقة بال signal transduction لكن كما
+
+415
+00:30:57,740 --> 00:31:02,340
+ذكرتلكم لأ لا يوجد هناك اثبات حقيقي لهذا الدور لإن
+
+416
+00:31:02,340 --> 00:31:07,060
+ال mice اللي مافيهاش PRP normal برضه ما اتأثرت
+
+417
+00:31:07,060 --> 00:31:11,360
+بهذا البرنامج في بعض الروابط و الفيديوهات اللي في
+
+418
+00:31:11,360 --> 00:31:15,100
+نهاية المحاضرة ممكن تشوفوهم على اليوتيوب ونكمل ان
+
+419
+00:31:15,100 --> 00:31:16,160
+شاء الله يوم الأربع
+
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/PLnuv8-yAaY_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/PLnuv8-yAaY_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..97b4cdfab94d571764d1818a85bb9f0f99cd2068
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/PLnuv8-yAaY_raw.json
@@ -0,0 +1 @@
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nucleic acids و ال nucleic acid هي عبارة عن RNA and DNA بعكس الفيروسات اللي ما تحدثنا كنا الفيروسات يا إما DNA يا إما RNA", "tokens": [37746, 6027, 2655, 6027, 1829, 47525, 8315, 8717, 3615, 28480, 21136, 3555, 3555, 32239, 8592, 32771, 13546, 12174, 16758, 37977, 24976, 9381, 3555, 5718, 118, 3660, 8717, 19913, 23557, 45164, 7435, 23328, 23032, 3555, 28671, 9154, 2423, 17352, 88, 8280, 3742, 8978, 29538, 33962, 15040, 25528, 23032, 3555, 3615, 995, 8978, 16472, 16254, 9122, 2304, 7649, 14962, 299, 21667, 4032, 2423, 14962, 299, 8258, 39896, 6225, 3555, 9640, 3660, 18871, 22484, 293, 8272, 45030, 4117, 3794, 27188, 13546, 41779, 9307, 13672, 1829, 19446, 6055, 24401, 12984, 8315, 9122, 8315, 27188, 13546, 41779, 9307, 35186, 11933, 15042, 8272, 35186, 11933, 15042, 22484], "avg_logprob": -0.25888480976516126, "compression_ratio": 1.6936936936936937, "no_speech_prob": 0.0, "words": [{"start": 199.82, "end": 200.66, "word": "وبالتالي", "probability": 0.9544921875}, {"start": 200.66, 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"probability": 0.974853515625}, {"start": 260.61, "end": 260.87, "word": " اللي", "probability": 0.95263671875}, {"start": 260.87, "end": 261.09, "word": " هو", "probability": 0.98583984375}, {"start": 261.09, "end": 261.31, "word": " ال", "probability": 0.94873046875}, {"start": 261.31, "end": 261.75, "word": " nitrogen", "probability": 0.9619140625}, {"start": 261.75, "end": 262.03, "word": " كمان", "probability": 0.9659830729166666}, {"start": 262.03, "end": 262.31, "word": " بشكل", "probability": 0.9542643229166666}, {"start": 262.31, "end": 262.67, "word": " 14", "probability": 0.86962890625}, {"start": 262.67, "end": 263.11, "word": "%", "probability": 0.95703125}, {"start": 263.11, "end": 263.85, "word": " من", "probability": 0.97802734375}, {"start": 263.85, "end": 265.09, "word": " الخلية", "probability": 0.9801432291666666}], "temperature": 1.0}, {"id": 12, "seek": 28602, "start": 265.73, "end": 286.03, "text": "في حين دي الهيدروجين تمانية في المية، في حين دي الفوسفوراس، وفي حين السلفر والبوتاسيوم والصوديوم بكميات أقل، إذا احنا متفقين على إنه تركيب الخلية تركيب الخلية قد ينظر إلى نظرات مختلفة وبأوجه مختلفة، وشوفنا أرقام اختلفت من لوحة إلى لوحة", "tokens": [41185, 11331, 9957, 11778, 1829, 2423, 3224, 25708, 2288, 29245, 9957, 46811, 7649, 10632, 8978, 9673, 10632, 12399, 8978, 11331, 9957, 11778, 1829, 27188, 41779, 5172, 13063, 32277, 12399, 4032, 41185, 11331, 9957, 21136, 46538, 2288, 16070, 3555, 35473, 32277, 1829, 20498, 16070, 9381, 2407, 16254, 20498, 4724, 24793, 1829, 9307, 5551, 4587, 1211, 12399, 11933, 15730, 1975, 5016, 8315, 44650, 5172, 4587, 9957, 15844, 36145, 3224, 6055, 31747, 1829, 3555, 33962, 1211, 10632, 6055, 31747, 1829, 3555, 33962, 1211, 10632, 12174, 3215, 7251, 1863, 19913, 2288, 30731, 8717, 19913, 2288, 9307, 3714, 46456, 46538, 3660, 46599, 10721, 29245, 3224, 3714, 46456, 46538, 3660, 12399, 4032, 8592, 38688, 8315, 5551, 2288, 4587, 10943, 1975, 46456, 46538, 2655, 9154, 45164, 5016, 3660, 30731, 45164, 5016, 3660], 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"probability": 0.5413818359375}, {"start": 275.75, "end": 277.17, "word": " تركيب", "probability": 0.98828125}, {"start": 277.17, "end": 278.07, "word": " الخلية", "probability": 0.9720052083333334}, {"start": 278.07, "end": 278.65, "word": " تركيب", "probability": 0.7945556640625}, {"start": 278.65, "end": 279.09, "word": " الخلية", "probability": 0.9666341145833334}, {"start": 279.09, "end": 279.39, "word": " قد", "probability": 0.990966796875}, {"start": 279.39, "end": 279.79, "word": " ينظر", "probability": 0.8505859375}, {"start": 279.79, "end": 280.05, "word": " إلى", "probability": 0.66064453125}, {"start": 280.05, "end": 280.81, "word": " نظرات", "probability": 0.8702392578125}, {"start": 280.81, "end": 281.57, "word": " مختلفة", "probability": 0.995361328125}, {"start": 281.57, "end": 282.59, "word": " وبأوجه", "probability": 0.819580078125}, {"start": 282.59, "end": 283.55, "word": " مختلفة،", "probability": 0.923828125}, {"start": 283.55, "end": 283.91, "word": " وشوفنا", "probability": 0.878173828125}, {"start": 283.91, "end": 284.25, "word": " أرقام", "probability": 0.8291015625}, {"start": 284.25, "end": 284.75, "word": " اختلفت", "probability": 0.866455078125}, {"start": 284.75, "end": 284.89, "word": " من", "probability": 0.99560546875}, {"start": 284.89, "end": 285.19, "word": " لوحة", "probability": 0.8523763020833334}, {"start": 285.19, "end": 285.39, "word": " إلى", "probability": 0.9462890625}, {"start": 285.39, "end": 286.03, "word": " لوحة", "probability": 0.9889322916666666}], "temperature": 1.0}, {"id": 13, "seek": 30600, "start": 288.04, "end": 306.0, "text": "ماعرف اللي هو حين كان اللي أمامكوا الصغيرة اللي الخطفية الصغيرة ان هي أبقى عنصورة و احنا يعني فرضناها خالص فصعب الرؤية لكن هحاول ان اخدر الإمكان انه اذكر او مرور سريع على المراكبات و اقولكوا المراكبات هذه وين موجودة فعشان بالمثال ان ال carbon", "tokens": [15042, 3615, 28480, 13672, 1829, 31439, 11331, 9957, 25961, 13672, 1829, 5551, 2304, 10943, 4117, 14407, 31767, 17082, 48923, 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quality السابقة اللي هو تتسخن و برد هناك بعض البكتيريا قد تنمو في هذا الغذاء و هتسبب food poisoning", "tokens": [37746, 6027, 2655, 6027, 1829, 1975, 8315, 3714, 11242, 9566, 2288, 1975, 5016, 5172, 19913, 6024, 118, 8848, 16606, 23758, 8978, 11778, 47341, 3660, 11331, 2288, 9640, 3660, 16490, 2304, 3794, 9957, 1975, 2407, 8608, 2655, 9957, 11778, 47341, 3660, 3714, 4587, 2407, 10632, 6225, 8592, 7649, 7251, 1211, 6027, 3224, 8608, 47283, 1863, 19446, 36081, 3794, 9778, 1863, 8592, 4724, 25720, 1975, 34740, 7578, 5296, 7649, 3224, 4724, 33911, 28543, 45030, 11242, 2423, 3125, 21136, 16758, 28671, 13672, 1829, 31439, 6055, 2655, 3794, 9778, 1863, 4032, 4724, 2288, 3215, 34105, 4117, 45030, 11242, 29739, 4117, 2655, 13546, 25528, 12174, 3215, 6055, 1863, 2304, 2407, 8978, 23758, 6024, 118, 8848, 16606, 4032, 8032, 2655, 35457, 3555, 1755, 36778], "avg_logprob": -0.21074054847244456, "compression_ratio": 1.6782608695652175, "no_speech_prob": 0.0, "words": [{"start": 1262.79, "end": 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{"id": 68, "seek": 165317, "start": 1639.47, "end": 1653.17, "text": "مين عمل فحص ال glucose؟ او حد ما وصلوش للفحوصات هذه في المختبأ؟ طيب الإنزيمات .. الإنزيمات احنا بنعملها فحص على pH محدد", "tokens": [2304, 9957, 6225, 42213, 6156, 5016, 9381, 2423, 23997, 22807, 1975, 2407, 11331, 3215, 19446, 4032, 36520, 2407, 8592, 24976, 5172, 5016, 2407, 9381, 9307, 29538, 8978, 9673, 46456, 3555, 10721, 22807, 23032, 1829, 3555, 33688, 1863, 11622, 32640, 9307, 4386, 33688, 1863, 11622, 32640, 9307, 1975, 5016, 8315, 44945, 25957, 1211, 11296, 6156, 5016, 9381, 15844, 21677, 3714, 24401, 3215], "avg_logprob": -0.270917349284695, "compression_ratio": 1.4236111111111112, "no_speech_prob": 0.0, "words": [{"start": 1639.47, "end": 1639.85, "word": "مين", "probability": 0.5904541015625}, {"start": 1639.85, "end": 1640.05, "word": " عمل", "probability": 0.966796875}, {"start": 1640.05, "end": 1640.37, "word": " فحص", "probability": 0.9853515625}, {"start": 1640.37, "end": 1640.49, "word": " ال", "probability": 0.36083984375}, {"start": 1640.49, "end": 1642.03, "word": " glucose؟", "probability": 0.634033203125}, {"start": 1642.03, "end": 1642.09, "word": " او", "probability": 0.6905517578125}, {"start": 1642.09, "end": 1642.51, "word": " حد", "probability": 0.662353515625}, {"start": 1642.51, "end": 1642.71, "word": " ما", "probability": 0.5634765625}, {"start": 1642.71, "end": 1643.17, "word": " وصلوش", "probability": 0.7236328125}, {"start": 1643.17, "end": 1643.93, "word": " للفحوصات", "probability": 0.8530680338541666}, {"start": 1643.93, "end": 1644.13, "word": " هذه", "probability": 0.5703125}, {"start": 1644.13, "end": 1644.27, "word": " في", "probability": 0.78515625}, {"start": 1644.27, "end": 1645.09, "word": " المختبأ؟", "probability": 0.72646484375}, {"start": 1645.09, "end": 1645.75, "word": " طيب", "probability": 0.845703125}, {"start": 1645.75, "end": 1647.75, "word": " الإنزيمات", "probability": 0.82138671875}, {"start": 1647.75, "end": 1647.87, "word": " ..", "probability": 0.5146484375}, {"start": 1647.87, "end": 1648.63, "word": " الإنزيمات", "probability": 0.9244140625}, {"start": 1648.63, "end": 1650.23, "word": " احنا", "probability": 0.800537109375}, {"start": 1650.23, "end": 1651.39, "word": " بنعملها", "probability": 0.9296875}, {"start": 1651.39, "end": 1651.85, "word": " فحص", "probability": 0.9969075520833334}, {"start": 1651.85, "end": 1652.13, "word": " على", "probability": 0.88818359375}, {"start": 1652.13, "end": 1652.55, "word": " pH", "probability": 0.364501953125}, {"start": 1652.55, "end": 1653.17, "word": " محدد", "probability": 0.984375}], "temperature": 1.0}, {"id": 69, "seek": 167674, "start": 1654.54, "end": 1676.74, "text": "هذا ال pH هو ال optimum معناه الحاجة ان في عندها مرضوية minimum و في عندها maximum below which لذلك لما احنا بنقول ان ال pH غير ملائم هو غالبا غير ملائم لمين ده الإنزيمات اللي موجودة في الخليل هناك بعض أنواع من الميكروبات بتحب ال pH المتعدد", "tokens": [3224, 15730, 2423, 21677, 31439, 2423, 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"word": " هو", "probability": 0.96484375}, {"start": 1655.46, "end": 1655.56, "word": " ال", "probability": 0.85888671875}, {"start": 1655.56, "end": 1656.0, "word": " optimum", "probability": 0.9814453125}, {"start": 1656.0, "end": 1656.34, "word": " معناه", "probability": 0.6640625}, {"start": 1656.34, "end": 1656.54, "word": " الحاجة", "probability": 0.5218912760416666}, {"start": 1656.54, "end": 1656.62, "word": " ان", "probability": 0.388427734375}, {"start": 1656.62, "end": 1656.72, "word": " في", "probability": 0.740234375}, {"start": 1656.72, "end": 1656.96, "word": " عندها", "probability": 0.795654296875}, {"start": 1656.96, "end": 1657.52, "word": " مرضوية", "probability": 0.8323974609375}, {"start": 1657.52, "end": 1658.24, "word": " minimum", "probability": 0.84326171875}, {"start": 1658.24, "end": 1658.64, "word": " و", "probability": 0.966796875}, {"start": 1658.64, "end": 1658.76, "word": " في", "probability": 0.64306640625}, {"start": 1658.76, "end": 1659.22, "word": " عندها", "probability": 0.987548828125}, {"start": 1659.22, "end": 1660.18, "word": " maximum", "probability": 0.9599609375}, {"start": 1660.18, "end": 1660.84, "word": " below", "probability": 0.452392578125}, {"start": 1660.84, "end": 1661.44, "word": " which", "probability": 0.91015625}, {"start": 1661.44, "end": 1662.34, "word": " لذلك", "probability": 0.70654296875}, {"start": 1662.34, "end": 1662.78, "word": " لما", "probability": 0.844482421875}, {"start": 1662.78, "end": 1663.2, "word": " احنا", "probability": 0.9220377604166666}, {"start": 1663.2, "end": 1663.9, "word": " بنقول", "probability": 0.882080078125}, {"start": 1663.9, "end": 1664.06, "word": " ان", "probability": 0.85791015625}, {"start": 1664.06, "end": 1664.2, "word": " ال", "probability": 0.94873046875}, {"start": 1664.2, "end": 1664.54, "word": " pH", "probability": 0.98291015625}, {"start": 1664.54, "end": 1664.86, "word": " غير", "probability": 0.997314453125}, {"start": 1664.86, "end": 1665.48, "word": " ملائم", "probability": 0.9664306640625}, {"start": 1665.48, "end": 1665.82, "word": " هو", "probability": 0.9404296875}, {"start": 1665.82, "end": 1666.3, "word": " غالبا", "probability": 0.9918212890625}, {"start": 1666.3, "end": 1666.66, "word": " غير", "probability": 0.989990234375}, {"start": 1666.66, "end": 1666.92, "word": " ملائم", "probability": 0.9813232421875}, {"start": 1666.92, "end": 1667.32, "word": " لمين", "probability": 0.804931640625}, {"start": 1667.32, "end": 1667.8, "word": " ده", "probability": 0.54150390625}, {"start": 1667.8, "end": 1668.62, "word": " الإنزيمات", "probability": 0.7309326171875}, {"start": 1668.62, "end": 1669.22, "word": " اللي", "probability": 0.6890869140625}, {"start": 1669.22, "end": 1669.6, "word": " موجودة", "probability": 0.983642578125}, {"start": 1669.6, "end": 1669.8, "word": " في", "probability": 0.9697265625}, {"start": 1669.8, "end": 1670.6, "word": " الخليل", "probability": 0.7965494791666666}, {"start": 1670.6, "end": 1672.28, 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"text": "مش الأرهانة هذه micro aerophilic هوائية لكن بدها كمية oxygen قليلة الرابع اللي قريب من اللي لا هوائية بقولنا لا هوائية وسميناها aerotolerant anaerobic يعني لا هوائية ولكن تتحمل وجود ال oxygen فسنعمل خمسة ان شاء الله التالتة هي بكتيريا هوائية بكتيريا هوائية", "tokens": [2304, 8592, 16247, 2288, 3224, 7649, 3660, 29538, 4532, 11207, 5317, 388, 299, 31439, 16373, 10632, 44381, 47525, 11296, 9122, 2304, 10632, 9169, 12174, 20292, 37977, 34892, 16758, 3615, 13672, 1829, 12174, 16572, 3555, 9154, 13672, 1829, 20193, 31439, 16373, 10632, 4724, 39648, 8315, 20193, 31439, 16373, 10632, 4032, 38251, 1829, 8315, 11296, 11207, 310, 27035, 394, 34178, 260, 22234, 37495, 22653, 20193, 31439, 16373, 10632, 35525, 19452, 6055, 2655, 35571, 1211, 49610, 23328, 2423, 9169, 6156, 3794, 1863, 25957, 1211, 16490, 2304, 3794, 3660, 16472, 13412, 16606, 21984, 16712, 6027, 2655, 3660, 39896, 4724, 4117, 2655, 13546, 25528, 31439, 16373, 10632, 4724, 4117, 2655, 13546, 25528, 31439, 16373, 10632], 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شغل، لو أنا معاه جزئي جلوكوز و أريد أن أعمله oxidation أو metabolism، متابوليزم دعوني أقول مصطلح أدق، لو عملت له متابوليزم في ورود الأكسجين، سأحصل على 36 ATP", "tokens": [2304, 45636, 995, 37495, 22653, 6055, 6027, 2655, 3660, 29739, 4117, 2655, 13546, 25528, 2423, 3224, 2407, 16373, 10632, 22807, 6055, 14851, 9566, 40228, 14739, 6055, 3615, 1829, 8592, 8978, 4032, 2288, 23328, 2423, 3224, 2407, 16606, 4032, 6225, 40448, 4032, 2288, 23328, 2423, 3224, 2407, 16606, 6055, 14851, 17082, 1211, 16247, 4117, 3794, 7435, 9957, 4032, 6055, 14851, 17082, 1211, 3714, 23557, 4117, 3555, 9307, 5551, 34740, 7578, 8978, 16712, 1863, 36178, 12399, 46811, 10943, 22807, 44381, 16490, 3215, 14407, 20666, 24793, 9154, 13412, 17082, 1211, 12399, 45164, 41850, 20449, 40294, 10874, 11622, 19986, 1829, 10874, 1211, 2407, 4117, 2407, 11622, 4032, 5551, 16572, 3215, 14739, 5551, 25957, 43761, 36767, 34051, 1131, 14923, 1434, 12399, 44650, 16758, 12610, 1829, 11622, 2304, 11778, 3615, 11536, 1829, 5551, 39648, 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"word": " O2", "probability": 0.8916015625}, {"start": 2557.51, "end": 2557.97, "word": " لاحظوا", "probability": 0.79949951171875}, {"start": 2557.97, "end": 2558.39, "word": " شوية", "probability": 0.8935546875}, {"start": 2558.39, "end": 2560.49, "word": " الـ2O2", "probability": 0.662109375}, {"start": 2560.49, "end": 2560.79, "word": " ها", "probability": 0.463623046875}, {"start": 2560.79, "end": 2561.01, "word": " دى", "probability": 0.623779296875}, {"start": 2561.01, "end": 2561.61, "word": " اللى", "probability": 0.80859375}, {"start": 2561.61, "end": 2561.97, "word": " فوقيها", "probability": 0.66864013671875}, {"start": 2561.97, "end": 2562.71, "word": " شحطة", "probability": 0.79132080078125}, {"start": 2562.71, "end": 2563.31, "word": " هفير", "probability": 0.5974934895833334}, {"start": 2563.31, "end": 2563.65, "word": " شحطة", "probability": 0.946533203125}, {"start": 2563.65, "end": 2563.79, "word": " ها", "probability": 0.5830078125}, {"start": 2563.79, "end": 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"temperature": 1.0}, {"id": 110, "seek": 269278, "start": 2664.24, "end": 2692.78, "text": "راح منه الهيدروجين فصار لنا إيه؟ مؤكسد لكن ما طلعش Oxygen بمعنى أخر المحصلة النهائية ال hydrogen peroxide فى التانية اتحول لمين؟ لماء فى الأولى اتحول لماء زاد Oxygen و فى التالى اتحول ماء إلى مركب مؤكسد بجكال مختصر و اتأكسد فى الحالتين خلصنا من صميم ال hydrogen peroxide", "tokens": [23557, 5016, 9154, 3224, 2423, 3224, 25708, 2288, 29245, 9957, 6156, 9381, 9640, 5296, 8315, 11933, 1829, 3224, 22807, 3714, 33604, 4117, 3794, 3215, 44381, 19446, 23032, 1211, 3615, 8592, 16489, 8647, 4724, 2304, 3615, 1863, 7578, 5551, 34740, 9673, 5016, 9381, 37977, 28239, 3224, 16373, 10632, 2423, 12697, 4768, 34454, 6156, 7578, 16712, 7649, 10632, 1975, 2655, 5016, 12610, 32767, 9957, 22807, 32767, 16606, 6156, 7578, 16247, 12610, 7578, 1975, 2655, 5016, 12610, 32767, 16606, 30767, 18513, 16489, 8647, 4032, 6156, 7578, 16712, 6027, 7578, 1975, 2655, 5016, 12610, 3714, 16606, 30731, 3714, 31747, 3555, 3714, 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0.90185546875}, {"start": 3075.82, "end": 3076.28, "word": " غالبا", "probability": 0.98876953125}, {"start": 3076.28, "end": 3076.64, "word": " بنقول", "probability": 0.762939453125}, {"start": 3076.64, "end": 3076.8, "word": " ل", "probability": 0.58203125}, {"start": 3076.8, "end": 3077.2, "word": " مين؟", "probability": 0.7718912760416666}, {"start": 3077.2, "end": 3077.38, "word": " لل", "probability": 0.70458984375}, {"start": 3077.38, "end": 3078.0, "word": " optimum", "probability": 0.9794921875}, {"start": 3078.0, "end": 3079.06, "word": " يعني", "probability": 0.91748046875}, {"start": 3079.06, "end": 3079.72, "word": " أفضل", "probability": 0.98779296875}, {"start": 3079.72, "end": 3080.44, "word": " الظروف", "probability": 0.97021484375}, {"start": 3080.44, "end": 3081.06, "word": " بنوفرها", "probability": 0.896484375}, {"start": 3081.06, "end": 3081.62, "word": " للبكتيريا", "probability": 0.936767578125}, {"start": 3081.62, "end": 3082.02, "word": " و", "probability": 0.6015625}, {"start": 3082.02, "end": 3082.34, "word": " بنحسب", "probability": 0.9593098958333334}, {"start": 3082.34, "end": 3082.48, "word": " ال", "probability": 0.8056640625}, {"start": 3082.48, "end": 3082.9, "word": " generation", "probability": 0.9658203125}, {"start": 3082.9, "end": 3083.72, "word": " time", "probability": 0.92041015625}, {"start": 3083.72, "end": 3084.02, "word": " و", "probability": 0.75146484375}, {"start": 3084.02, "end": 3084.3, "word": " أقول", "probability": 0.891845703125}, {"start": 3084.3, "end": 3084.7, "word": " لكم", "probability": 0.923583984375}, {"start": 3084.7, "end": 3085.56, "word": " أنه", "probability": 0.6004638671875}, {"start": 3085.56, "end": 3085.78, "word": " في", "probability": 0.9677734375}, {"start": 3085.78, "end": 3086.04, "word": " بعض", "probability": 0.988525390625}, {"start": 3086.04, "end": 3086.26, "word": " أنواع", "probability": 0.9602864583333334}, {"start": 3086.26, "end": 3086.4, "word": " من", "probability": 0.5986328125}, {"start": 3086.4, "end": 3086.92, "word": " البكتيريا", "probability": 0.9859375}, {"start": 3086.92, "end": 3087.4, "word": " بنختمها", "probability": 0.6788330078125}, {"start": 3087.4, "end": 3087.96, "word": " للمعلومة", "probability": 0.837158203125}, {"start": 3087.96, "end": 3088.48, "word": " ال", "probability": 0.94775390625}, {"start": 3088.48, "end": 3088.92, "word": " generation", "probability": 0.9765625}, {"start": 3088.92, "end": 3089.54, "word": " time", "probability": 0.92236328125}, {"start": 3089.54, "end": 3090.14, "word": " قد", "probability": 0.97802734375}, {"start": 3090.14, "end": 3090.34, "word": " يصل", "probability": 0.962158203125}, {"start": 3090.34, "end": 3090.58, "word": " إلى", "probability": 0.794921875}, {"start": 3090.58, "end": 3091.06, "word": " فقط", "probability": 0.980712890625}, {"start": 3091.06, "end": 3091.3, "word": " عشر", "probability": 0.76318359375}, {"start": 3091.3, "end": 3091.88, "word": " دقائق", "probability": 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\ No newline at end of file
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/PLnuv8-yAaY_raw.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/PLnuv8-yAaY_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..ce94bf494d1632bd96c720c3121147bcc6c3ccc0
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/PLnuv8-yAaY_raw.srt
@@ -0,0 +1,2616 @@
+1
+00:00:21,280 --> 00:00:24,860
+بسم الله الرحمن الرحيم هتكلم عن ال bacterial
+
+2
+00:00:24,860 --> 00:00:32,400
+physiology و هي مناسبة للموضوع
+
+3
+00:00:32,400 --> 00:00:35,420
+اللي أنهيناه اللي هو ال bacterial cell structure
+
+4
+00:00:35,420 --> 00:00:38,040
+وبالتالي من المناسب انه نبدأ ان شاء الله المخاطرة
+
+5
+00:00:38,040 --> 00:00:40,380
+بأنواع ال bacterial physiology
+
+6
+00:00:43,590 --> 00:00:47,450
+كأي خلية في الدنيا هناك مكونات لهذه الخلية غالبا
+
+7
+00:00:47,450 --> 00:00:51,510
+نستطيع أنه نصنفها بتصنيفات مختلفة مثلا ك chemical
+
+8
+00:00:51,510 --> 00:00:55,570
+component فيها ال water لكن ال water بدنا نغير
+
+9
+00:00:55,570 --> 00:00:58,790
+مفهومنا عنه اليوم نتكلم عنه علم ال water free
+
+10
+00:00:58,790 --> 00:01:04,690
+water و bound water يعني قد نرى بعض الأطعمة على
+
+11
+00:01:04,690 --> 00:01:08,190
+سبيل المثال المربع المربع بحتوي كمية كبيرة من
+
+12
+00:01:08,190 --> 00:01:12,760
+الماءJelly-like يعني واضح أنه من القوام تبعه أنه
+
+13
+00:01:12,760 --> 00:01:16,740
+هيحتفظ على كمية كبيرة من الماء لكن لو وضعنا
+
+14
+00:01:16,740 --> 00:01:21,160
+بكتيريا في ال jelly هلاجئ أنه البكتيريا غير قادرة
+
+15
+00:01:21,160 --> 00:01:26,180
+على النمو بالعكس الماء اللي جواها بتفقد لصالح مين؟
+
+16
+00:01:26,180 --> 00:01:30,020
+المربّى لأنه تركيز السكر عال جدا فيه فهذا يعتبر
+
+17
+00:01:30,020 --> 00:01:35,530
+bound water يعني ماء مرتبط غير متاح للبكتيرياالماء
+
+18
+00:01:35,530 --> 00:01:38,530
+المتاح اللي هو ال available water او ال free water
+
+19
+00:01:38,530 --> 00:01:42,730
+و يعبر عنه بما يسمى ال water activity water
+
+20
+00:01:42,730 --> 00:01:47,990
+activity شايفين الرمز هذا ال AWهذا بيعبر عن ما
+
+21
+00:01:47,990 --> 00:01:52,490
+يسمى بال water activity inorganic salts أملاح غير
+
+22
+00:01:52,490 --> 00:01:55,410
+عضوية زي الفوسفلاس والمغنيسيوم والكالسيوم
+
+23
+00:01:55,410 --> 00:02:01,450
+والسوديوم لبروتينات واتشكل كمية كبيرة من الوزن
+
+24
+00:02:01,450 --> 00:02:05,490
+الجاف للخلية حوالي خمسين ل تمانين بالمائة من وزن
+
+25
+00:02:05,490 --> 00:02:08,790
+الخلية الجاف وعبارة عن بروتينات وانا بتتصوروا
+
+26
+00:02:08,790 --> 00:02:12,620
+البروتينات مما تتكوّنبتكول لبروتينات من كربون و من
+
+27
+00:02:12,620 --> 00:02:15,840
+نيتروجين و من سلفر و من other components يعني
+
+28
+00:02:15,840 --> 00:02:21,380
+البروتين كمركب يشتمل على مركبات أخرى ال sugar أو
+
+29
+00:02:21,380 --> 00:02:28,580
+السكريات و أذكركم فقط بقضية ال .. ال .. ال .. ال
+
+30
+00:02:28,580 --> 00:02:32,120
+بتيدوجلايكان ال بتيدوجلايكان أصلا مكون من
+
+31
+00:02:32,120 --> 00:02:36,020
+inacetylglucosamine و inacetylmuramicacid والتنين
+
+32
+00:02:36,020 --> 00:02:41,180
+عبارة عن carbohydrates أو sugarsأيضا ال capsule
+
+33
+00:02:41,180 --> 00:02:44,560
+اللى بتتذكره المكونة كانت من polysaccharide كان
+
+34
+00:02:44,560 --> 00:02:49,380
+فينا ال lipids او الدهون فاكرين ال phospholipids
+
+35
+00:02:49,380 --> 00:02:54,620
+ال cell membrane و كان في ال outer membrane تبع ال
+
+36
+00:02:54,620 --> 00:02:57,360
+gram negative بكتيريا برضه كان فينا outer membrane
+
+37
+00:02:57,360 --> 00:03:02,000
+و مكون من اللي هو ال lipids طبعا في بعض البكتيريا
+
+38
+00:03:02,000 --> 00:03:05,980
+فيها fats أخرى بتكون فيها waxy lipids
+
+39
+00:03:09,380 --> 00:03:12,860
+دهون شمية، و تذكر هذه الجملة كويس لإنه هنستفيد
+
+40
+00:03:12,860 --> 00:03:16,140
+منها لما نتكلم عن ال acid fast stain، هناك بعض
+
+41
+00:03:16,140 --> 00:03:18,880
+أنواع من الباتيريا غير قابلة للصداقة بال gram
+
+42
+00:03:18,880 --> 00:03:23,340
+stainوبالتالي بدنا نعرف السبب ليش غير قابلة
+
+43
+00:03:23,340 --> 00:03:28,360
+للصباغة نظرا لوجود طبقة من ال waxy lipids في هذه
+
+44
+00:03:28,360 --> 00:03:31,500
+الخلايا طبعا في اندي كمان nucleic acids و ال
+
+45
+00:03:31,500 --> 00:03:36,080
+nucleic acid هي عبارة عن RNA and DNA بعكس
+
+46
+00:03:36,080 --> 00:03:39,720
+الفيروسات اللي ما تحدثنا كنا الفيروسات يا إما DNA
+
+47
+00:03:39,720 --> 00:03:41,320
+يا إما RNA
+
+48
+00:03:44,200 --> 00:03:49,160
+نظرة أخرى بطريقة أخرى على البكتيريا ياريتنا نركز
+
+49
+00:03:49,160 --> 00:03:53,380
+على اللوحة و نشوف أنه اللوحة بتقولنا أنه 50% من
+
+50
+00:03:53,380 --> 00:04:00,410
+وزن البكتيريا اللي جاء هو carbon60% ل 80% البروتين
+
+51
+00:04:00,410 --> 00:04:05,010
+موضوع كله البروتين تعبير اخر مختلف عن كلمة carbon
+
+52
+00:04:05,010 --> 00:04:08,510
+البروتين يحتوي على carbon و غير ال carbon لكن هنا
+
+53
+00:04:08,510 --> 00:04:11,290
+نتكلم عن ال carbon و ال carbon قد يكون موجود في ال
+
+54
+00:04:11,290 --> 00:04:14,310
+carbohydrates و في ال limits و في ال proteins في
+
+55
+00:04:14,310 --> 00:04:17,910
+عندي ال oxygen بيحتوي برضه او نسبة كبيرة من الخلية
+
+56
+00:04:17,910 --> 00:04:21,750
+هي عبارة عن oxygen في عندي اللي هو ال nitrogen
+
+57
+00:04:21,750 --> 00:04:26,950
+كمان بشكل 14% من الخليةفي حين دي الهيدروجين تمانية
+
+58
+00:04:26,950 --> 00:04:30,110
+في المية، في حين دي الفوسفوراس، وفي حين السلفر
+
+59
+00:04:30,110 --> 00:04:34,630
+والبوتاسيوم والصوديوم بكميات أقل، إذا احنا متفقين
+
+60
+00:04:34,630 --> 00:04:40,050
+على إنه تركيب الخلية تركيب الخلية قد ينظر إلى
+
+61
+00:04:40,050 --> 00:04:44,890
+نظرات مختلفة وبأوجه مختلفة، وشوفنا أرقام اختلفت من
+
+62
+00:04:44,890 --> 00:04:49,420
+لوحة إلى لوحةماعرف اللي هو حين كان اللي أمامكوا
+
+63
+00:04:49,420 --> 00:04:51,860
+الصغيرة اللي الخطفية الصغيرة ان هي أبقى عنصورة و
+
+64
+00:04:51,860 --> 00:04:56,120
+احنا يعني فرضناها خالص فصعب الرؤية لكن هحاول ان
+
+65
+00:04:56,120 --> 00:05:01,100
+اخدر الإمكان انه اذكر او مرور سريع على المراكبات و
+
+66
+00:05:01,100 --> 00:05:05,300
+اقولكوا المراكبات هذه وين موجودة فعشان بالمثال ان
+
+67
+00:05:05,300 --> 00:05:08,410
+ال carbonأنا قلت الكربون مولود في وين؟ في
+
+68
+00:05:08,410 --> 00:05:10,990
+البروتين، مولود في الكربوهادرياس، مولود في
+
+69
+00:05:10,990 --> 00:05:14,950
+الليبتز، موجود حتى في النوكليك أسيت وبالتالي
+
+70
+00:05:14,950 --> 00:05:18,490
+الكربون هو أكتر أنصر و احنا اعطيناها خمسين بالمية
+
+71
+00:05:18,490 --> 00:05:23,810
+من وزن الخلية الجاف، هنا طبعا انا مهم جدا نعرف
+
+72
+00:05:23,810 --> 00:05:26,910
+الكربون وين موجود ومهم جدا نعرف وين النيترويدين
+
+73
+00:05:26,910 --> 00:05:30,250
+مولود، اللي عارفين ليش؟ لأن بكرا هنقول انه في حد
+
+74
+00:05:30,250 --> 00:05:35,960
+ال media، وسط الزراعي، هذا الوسط الزراعيبدنا نوفر
+
+75
+00:05:35,960 --> 00:05:40,760
+للبكتيريا اللى بنناميها مصادر كربونية مصادر
+
+76
+00:05:40,760 --> 00:05:43,840
+نيتروجينية بدنا نوفر لها minerals بدنا نوفر لها
+
+77
+00:05:43,840 --> 00:05:48,380
+water water activity كافية مناسبة فمعرفتي
+
+78
+00:05:48,380 --> 00:05:53,400
+بالمركبات المحتوية على تلك العناصر بد انا اصنع ال
+
+79
+00:05:53,400 --> 00:05:56,540
+media تبعتي بدون هذه المعرفة لن استطيع ان اقوم
+
+80
+00:05:56,540 --> 00:06:00,920
+بتصنية اللى هو ال media فمهم جدا ان اعرف الكلامعند
+
+81
+00:06:00,920 --> 00:06:05,380
+الأكسجين والأهم مصدر له في الخلية هو مين ال water
+
+82
+00:06:05,380 --> 00:06:10,980
+ال H2O كلكم بتعرفوا أن الوزن الرطب للخلية قد يشكل
+
+83
+00:06:10,980 --> 00:06:17,920
+70% ال water من الواق الخلية ال nitrogen والنيترجن
+
+84
+00:06:17,920 --> 00:06:22,700
+أذكركم بمراكبات بتعرفوها كويس و بتحتوى على نيترجن
+
+85
+00:06:22,700 --> 00:06:27,250
+واحدماذا يسمى ال .. ال .. ال .. ال .. ال
+
+86
+00:06:27,250 --> 00:06:30,870
+nitrogenous bases قواعد النيتروجينية اللي هم
+
+87
+00:06:30,870 --> 00:06:36,150
+الأدينين والجوانين والسايدوسين واليوراسين
+
+88
+00:06:36,150 --> 00:06:41,310
+والثايمين نعم هدولة كلهم قواعد نيتروجينية ال amino
+
+89
+00:06:41,310 --> 00:06:44,930
+acids اسمه amino acid لإنه فيه amino group و ال
+
+90
+00:06:44,930 --> 00:06:49,010
+amino group هو ال H2 مظبوط ال H2 يبقى ال nitrogen
+
+91
+00:06:49,010 --> 00:06:52,110
+موجود في ال amino acids يعني في البروتيناتإذا
+
+92
+00:06:52,110 --> 00:06:55,550
+قواعد نيتروجينية و protein و amino acids هذي
+
+93
+00:06:55,550 --> 00:06:59,790
+المصدر الأيسرانيين للنيتروجين فينا ال .. ال
+
+94
+00:06:59,790 --> 00:07:03,110
+hydrogen برضه المصدر الأساسي اللي هو الماء لإن هو
+
+95
+00:07:03,110 --> 00:07:08,720
+للوكسيجين و لل .. فينا ال phosphorusفوسفرس تذكروا
+
+96
+00:07:08,720 --> 00:07:12,920
+ان ال backbone تبع المادة الوراثية الو ديانات في
+
+97
+00:07:12,920 --> 00:07:17,340
+فوسفرس في phosphate group في phosphate group تذكر
+
+98
+00:07:17,340 --> 00:07:20,940
+ال phospholipids اسمها phospholipids يبقى فيها
+
+99
+00:07:20,940 --> 00:07:27,630
+انها ايضا phosphate groupالسلفر و اذكركم ان السلفر
+
+100
+00:07:27,630 --> 00:07:33,610
+اسمها sulfur containing amino acids يعني التصنيف
+
+101
+00:07:33,610 --> 00:07:37,290
+من تصنيفات ال amino acids ان فيها كبريه خدوا بالكم
+
+102
+00:07:37,290 --> 00:07:42,710
+هذه الكميات و المصادر قد تختلف من microب ل microب
+
+103
+00:07:42,710 --> 00:07:49,730
+بمعنى بكتيريا تعيش في قاع المحيطات لا تمتلك oxygen
+
+104
+00:07:49,730 --> 00:07:55,390
+molecular جزائي البتةلأ تبتلك حتى إضاءة عارفين
+
+105
+00:07:55,390 --> 00:07:58,230
+الأنصر الأساسي إيه لها التنفس هو ال sulfur
+
+106
+00:07:58,230 --> 00:08:02,990
+الكابريد فهي بحاجة إلى كميات كبيرة من من الكابريد
+
+107
+00:08:02,990 --> 00:08:06,250
+بالنسبة للبكتيريا العادية الممرضة لأنها حالتها
+
+108
+00:08:06,250 --> 00:08:10,210
+الكابريد بتكون حاجة قليلة جدا حاجة قليلة جدا
+
+109
+00:08:11,160 --> 00:08:14,820
+البوتاسيوم والبوتاسيوم عبارة عن كوفاكتور ايضا كمان
+
+110
+00:08:14,820 --> 00:08:18,200
+المجنيسيوم والكالسيوم والايرون كلهم هدولة نحتاجهم
+
+111
+00:08:18,200 --> 00:08:21,380
+بكميات قليلة وبيعملوا ك كوفاكتور وممكن يكونوا
+
+112
+00:08:21,380 --> 00:08:25,420
+موجودين في انزايم محددين واشهر مثال الايرون
+
+113
+00:08:25,420 --> 00:08:29,060
+الايرون موجود في مجموعة من الانزيمات اسمها
+
+114
+00:08:29,060 --> 00:08:35,420
+cytochromes والcytochromes هي مجموعة الانزيمات
+
+115
+00:08:35,420 --> 00:08:42,280
+اللازمة لعملية الاكسدة في الخلية وانتاج الطباللوحة
+
+116
+00:08:42,280 --> 00:08:48,440
+اللي أمامنا الآن لوحة معبرة وفيها حجم معلومات ضخم
+
+117
+00:08:48,440 --> 00:08:56,780
+جدا يمكن توقف عندها فترات طويلة لأن كل نقطة من هذه
+
+118
+00:08:56,780 --> 00:09:00,940
+النقاط يعني هي بحد ذاتها قد تكون فصل او فاصلين في
+
+119
+00:09:00,940 --> 00:09:04,620
+كتاب ال nutrients ال temperature ال hydrogen ion
+
+120
+00:09:04,620 --> 00:09:08,020
+concentration او ال VH ال oxygen requirement وال
+
+121
+00:09:08,020 --> 00:09:14,530
+osmotic pressureناخد واحدة واحدة ونحاول انه بقدر
+
+122
+00:09:14,530 --> 00:09:17,330
+المقتصر المفيد لان احنا قلنا essential
+
+123
+00:09:17,330 --> 00:09:21,790
+microbiology نحاول نقلل من حجم المعلومات لكن نعطي
+
+124
+00:09:21,790 --> 00:09:24,010
+معلومات مفيدة اننا في ال diagnostic microbiome ان
+
+125
+00:09:24,010 --> 00:09:31,090
+شاء الله ناخد اول معلومة ليها ال nutrients احنا
+
+126
+00:09:31,090 --> 00:09:38,240
+ممكن نصنف البكتيريا بحسب حاجتها للغذاءأو بطريقتها
+
+127
+00:09:38,240 --> 00:09:43,060
+في إنتاج أو استهلاك الغذاء إلى سنتين Autotroph ده
+
+128
+00:09:43,060 --> 00:09:47,880
+دي التغبية وهي يعني لن نتطرق لها في ال medical
+
+129
+00:09:47,880 --> 00:09:51,440
+microbiology لأن كل ميكروباتنا احنا من نوع تاني
+
+130
+00:09:51,440 --> 00:09:55,440
+اللي هي ال heterotroph و ال heterotroph they
+
+131
+00:09:55,440 --> 00:10:02,140
+utilize ready made food يعني أغبية معدة سلفا معدة
+
+132
+00:10:02,140 --> 00:10:06,740
+و جاهزة من الآخرين ال autotrophs تشبه النباتات
+
+133
+00:10:07,550 --> 00:10:12,070
+Photosynthetic بتصنع من الإضاءة و الـCO2
+
+134
+00:10:12,070 --> 00:10:17,190
+Carbohydrates تمام؟ فهي بيسموها Primary Producers،
+
+135
+00:10:17,190 --> 00:10:21,530
+منتجة، يعني مخلوقات منتجة، اللي أهميتها البيئية في
+
+136
+00:10:21,530 --> 00:10:24,970
+كوكب الأرض لكن من ناحية طبيعية ليس لنا بها أي
+
+137
+00:10:24,970 --> 00:10:29,850
+علاقة، حتى الـHeterotroph بنصنفهم إلى صينفين
+
+138
+00:10:31,090 --> 00:10:37,070
+Saprophytes وهي باللغة العربية مترمنة وكلمة مترمنة
+
+139
+00:10:37,070 --> 00:10:42,170
+تطلق على الكائنات اللى تتغذى على مخلوقات ميتة على
+
+140
+00:10:42,170 --> 00:10:46,750
+مخلوقات ميتة النوع التانى اللى هو البرازيتك او
+
+141
+00:10:46,750 --> 00:10:49,950
+البرازايتس وهى اللى بتطفل على المخلوقات الحياة
+
+142
+00:10:49,950 --> 00:10:56,060
+اللى بتشمل الميكروبات اللى بتضرنا احنا كبشرإذا
+
+143
+00:10:56,060 --> 00:10:59,980
+كمان مرة Autotroph وHeterotroph وHeterotroph تسمان
+
+144
+00:10:59,980 --> 00:11:03,980
+اللي هم ال Sabrophytes والتاني يقولنا ال parasites
+
+145
+00:11:03,980 --> 00:11:07,340
+طيب
+
+146
+00:11:07,340 --> 00:11:10,920
+ال nutrients ال requirement بشكل عام للبكتيريا
+
+147
+00:11:10,920 --> 00:11:14,740
+تتلخص في مجموعة من العناصر الرئيسية ال water
+
+148
+00:11:14,740 --> 00:11:21,460
+وتخيلوا إنه قد أحتاج إلى تسعين بالمية من المكونات
+
+149
+00:11:21,460 --> 00:11:26,370
+تكون water تفكرين كلمة water activity؟كمان مرة
+
+150
+00:11:26,370 --> 00:11:31,650
+water activity بتعبر عن مين؟ عن ال free water ال
+
+151
+00:11:31,650 --> 00:11:35,290
+requirement للميكروبات تختلف وبالتالي عندما يطلب
+
+152
+00:11:35,290 --> 00:11:39,150
+مني انا ان اتعامل مع ميكروب انا ما بعرفه، وش دي؟
+
+153
+00:11:39,150 --> 00:11:42,530
+جالي لبنان حاضر له وصد؟ وصد، من الأشياء اللي لازم
+
+154
+00:11:42,530 --> 00:11:45,810
+ادرسها عن هذا الميكروب هو ال water activity
+
+155
+00:11:45,810 --> 00:11:50,130
+requirement كده بده water activity؟أو على الأقل
+
+156
+00:11:50,130 --> 00:11:55,050
+أعرف المعلومة هذه عشان أنا ممكن أفكر بطريقة تانية،
+
+157
+00:11:55,050 --> 00:11:58,470
+أقول هذا الميكروبط أنا بتعزله من بيئة، البيئة
+
+158
+00:11:58,470 --> 00:12:03,050
+بالمناسبة انتوا رايين اليوم دخلت القاعة من المبنى
+
+159
+00:12:03,050 --> 00:12:07,320
+شميته رؤحت المطر الجديدة؟رياح دي المطر الجديدة دي
+
+160
+00:12:07,320 --> 00:12:11,640
+عبارة راحة Actinomycetes بكتيريا طبعا بتشموها فقط
+
+161
+00:12:11,640 --> 00:12:15,920
+في .. في أول موسم هي من .. من الميكروفات الرائعة
+
+162
+00:12:15,920 --> 00:12:19,420
+اللي بتنتجلنا كمية كبيرة من ال antibiotics و بتنتج
+
+163
+00:12:19,420 --> 00:12:22,300
+بعض الأحيان .. أو مش بعض الأحيان ال soil
+
+164
+00:12:22,300 --> 00:12:27,440
+actinomycetes بتنتج اللي هي ال aromatic substances
+
+165
+00:12:27,440 --> 00:12:33,080
+اللي احنا بنشتمها مع تفاعلها مع الوقم المطرفبس
+
+166
+00:12:33,080 --> 00:12:36,340
+حبينا نقولكوا إنه هذه الرائحة اللي .. اللي .. اللي
+
+167
+00:12:36,340 --> 00:12:39,460
+بتذكرنا ده أو .. أو ممكن نشتجلها لأنها بتمر على
+
+168
+00:12:39,460 --> 00:12:43,940
+سنة حتى نشتمها مرة تانية فهي رائحة ل .. ل ..
+
+169
+00:12:43,940 --> 00:12:48,120
+للنتجة عن البكتيريا water, carbon source, nitrogen
+
+170
+00:12:48,120 --> 00:12:51,140
+source, minerals, growth factors و ال growth
+
+171
+00:12:51,140 --> 00:12:55,900
+factors غالبا تطلق على عناصر البكتيريا غير قادرة
+
+172
+00:12:55,900 --> 00:12:59,180
+على أنها تصنعها وبالتالي بتحتاجها إن احنا نزود
+
+173
+00:12:59,180 --> 00:13:03,710
+الوسط فيهانرجح على ال water water activity كمان
+
+174
+00:13:03,710 --> 00:13:07,390
+مرة قلنا لكوا مطلوب نعرف عن ال microbe ال water
+
+175
+00:13:07,390 --> 00:13:13,950
+activity بدي احزل microbe X من التربة وكلمة تربة
+
+176
+00:13:13,950 --> 00:13:17,690
+معناها حاجة ان في منافسين كثر مع هذا ال microbe
+
+177
+00:13:19,420 --> 00:13:23,320
+فإذا علمت إن ال requirement تبع هذا ال microbe
+
+178
+00:13:23,320 --> 00:13:28,020
+unique، كلمة unique يعني فريدة، مميزة، أستطيع أن
+
+179
+00:13:28,020 --> 00:13:32,560
+أتحكم في الوسط، تمام؟، بحيث أن يلائم هذا ال
+
+180
+00:13:32,560 --> 00:13:35,840
+microbe ولا يلائم ميكروبيرات أخرى، مصطلح بنسمي
+
+181
+00:13:35,840 --> 00:13:41,000
+selective isolation، عزل انتقائي أو عزل اختياري
+
+182
+00:13:42,770 --> 00:13:47,210
+selective isolation وفي المختبر بنعمل تقنية اسمها
+
+183
+00:13:47,210 --> 00:13:51,490
+selective media أو بصدرية انتقائية هنتكلم عنها في
+
+184
+00:13:51,490 --> 00:13:56,870
+وقت اللحظة ان شاء الله طيب انتوا الآن لما انا بقول
+
+185
+00:13:56,870 --> 00:14:06,070
+carbon source ايش بختاروا على بالكم؟ اللوحة
+
+186
+00:14:06,070 --> 00:14:11,880
+السابقة اللوحة السابقةإن ال carbon موجود في ال
+
+187
+00:14:11,880 --> 00:14:15,680
+carbohydrate في ال proteins وبالتالي الآن .. الآن
+
+188
+00:14:15,680 --> 00:14:18,820
+إذا بدي carbon source بدي أعرف برضه حاجة البكتيريا
+
+189
+00:14:18,820 --> 00:14:24,360
+هل بيلزمها الجلوكوز فقط؟ هل بيلزمها starch؟ ال
+
+190
+00:14:24,360 --> 00:14:28,000
+starch أرخص من الجلوكوز وبتنموا ع التنين بحطلها
+
+191
+00:14:28,000 --> 00:14:33,520
+starch يبقى بدّا قيلها carbon source وغالبا one
+
+192
+00:14:33,520 --> 00:14:38,610
+single carbon source ملائم يكفي البكتيريا تلميهone
+
+193
+00:14:38,610 --> 00:14:41,990
+single carbon source يعني بحطولها جليكوز بحطولها
+
+194
+00:14:41,990 --> 00:14:45,810
+لكتوزة حسب نوعية البكتيريا ال nitrogen source برضه
+
+195
+00:14:45,810 --> 00:14:50,330
+لازم مصادره ال proteins digested amino acids افضل
+
+196
+00:14:50,330 --> 00:14:53,670
+digested proteins مكسرة ال amino acids نيتروجينس
+
+197
+00:14:53,670 --> 00:14:57,390
+بيس نوكليك اسد ممشي الحال خدوا بالك انا انا بتكلم
+
+198
+00:14:57,390 --> 00:15:03,150
+عن nitrogen sources من مصادر natural يعني organic
+
+199
+00:15:03,150 --> 00:15:07,070
+افضل ففي اني كمان مصادر اخرى inorganic زي ال
+
+200
+00:15:07,070 --> 00:15:15,500
+ammoniaالأمون يعيش رمزها in H4 البترات
+
+201
+00:15:15,500 --> 00:15:18,500
+ال O3 مظبوطة ولا لأ؟ ممكن برضه نضيفها، ففي عندي
+
+202
+00:15:18,500 --> 00:15:23,640
+مصدر نيتروجيني من organic sources و من inorganic
+
+203
+00:15:23,640 --> 00:15:27,400
+sources، جالبا نحتاج أن نعمل mixture، يعني بنحط
+
+204
+00:15:27,400 --> 00:15:31,260
+organic مع inorganic، بس ال carbon source كم واحد؟
+
+205
+00:15:32,090 --> 00:15:36,270
+carbon sources one carbon source ال minerals قلنا
+
+206
+00:15:36,270 --> 00:15:40,090
+هي عبارة عن تاكرين لما اتكلمنا عن مجموعة من
+
+207
+00:15:40,090 --> 00:15:44,450
+الكالسيوم و الفوسفرات هاد كلها عناصر تضعف بكميات
+
+208
+00:15:44,450 --> 00:15:48,330
+قليلة لإن البكتيريا بتكون بحاجتها بالفعل بكميات
+
+209
+00:15:48,330 --> 00:15:53,510
+قليلة و أعطيكم مثال بسيط على إنها لازم تكون بكميات
+
+210
+00:15:53,510 --> 00:15:56,250
+قليلة تعرفوا نحاس copper
+
+211
+00:15:59,750 --> 00:16:06,110
+essential لبعض ال enzymes في الخلية البكتيرية لكن
+
+212
+00:16:06,110 --> 00:16:10,690
+ال cover نفسه النحاس لو زادت كميته بيقتل البكتيريا
+
+213
+00:16:10,690 --> 00:16:15,330
+و اتعرف ان في قطعة معدنية مصنوعة من النحاس مصنوعة
+
+214
+00:16:15,330 --> 00:16:19,290
+من النحاس لو أخدنا قطعة معدنية مصنوعة من النحاس و
+
+215
+00:16:19,290 --> 00:16:22,590
+حطيناها على طبق فيه بكتيريا هلاجى حول البكتيريا
+
+216
+00:16:22,590 --> 00:16:24,950
+منطقة خالية من مادة البكتيريا
+
+217
+00:16:27,710 --> 00:16:32,070
+لكن required in trace amount بكميات ضئيلة جدا
+
+218
+00:16:32,070 --> 00:16:36,810
+النقطة الخاصة اللي قلنا عنها ال growth factors
+
+219
+00:16:36,810 --> 00:16:41,050
+that a cell must have for the growth but they
+
+220
+00:16:41,050 --> 00:16:46,590
+cannot synthesize by themselves بمعنى أنه لازم
+
+221
+00:16:46,590 --> 00:16:52,490
+أزود البكتيريا بهذه المكونات لأنها ما بتقدر تصنعها
+
+222
+00:16:52,490 --> 00:16:53,430
+بذلك
+
+223
+00:16:55,920 --> 00:16:59,500
+من ال growth factor بدنا ناخد معلومة باللون الأحمر
+
+224
+00:16:59,500 --> 00:17:05,400
+معمولة على اللوحة اسمها fastidious bacteria تركزوا
+
+225
+00:17:05,400 --> 00:17:08,400
+معايا في الجزئيها دي لإنه مهم جدا ال fastidious
+
+226
+00:17:08,400 --> 00:17:13,520
+bacteria هي بكتيريا تختلف
+
+227
+00:17:13,520 --> 00:17:16,060
+عن باقي أنواع البكتيريا او بتعطي مثال انا حاط هنا
+
+228
+00:17:16,060 --> 00:17:21,360
+على اللوحة بكتيريا اسمها hemophilus influenza
+
+229
+00:17:23,430 --> 00:17:27,710
+الاسم فقط لانه ظن في يوم من الأيام ان هي المسبب لل
+
+230
+00:17:27,710 --> 00:17:31,370
+influenza لانها عُزِلت من ألف الناس اللي كان عندهم
+
+231
+00:17:31,370 --> 00:17:34,790
+influenza وما كان الفيروس معروف في ذلك الوقت فأطلق
+
+232
+00:17:34,790 --> 00:17:37,910
+عليها Hemophilus influenza على ان هي المسبب لمين
+
+233
+00:17:37,910 --> 00:17:41,070
+لمرض ال influenza وبعد ذلك اكتشفت ان هو طبعا ال
+
+234
+00:17:41,070 --> 00:17:46,820
+virus المشهورالان هذه البكتيريا بتحتاج الى ..
+
+235
+00:17:46,820 --> 00:17:51,680
+مابتقدرش تعيش بدون two factors واحد بيسموه X واحد
+
+236
+00:17:51,680 --> 00:17:57,200
+بيسموه V factor وال X هو همين وال V factor هو
+
+237
+00:17:57,200 --> 00:18:04,320
+الناد فاكتور يبقى في عندي two compounds لازم أحطهم
+
+238
+00:18:04,320 --> 00:18:09,080
+في الوسط عشان البكتيريا هذه تقدر تم .. مابتقدرش
+
+239
+00:18:09,080 --> 00:18:13,160
+صنعهم بداليهامع العلم ان هدول الاتنين other
+
+240
+00:18:13,160 --> 00:18:18,720
+بكتيريا بتقدر تصنعهم other بكتيريا عادية بتصنعهم
+
+241
+00:18:18,720 --> 00:18:23,980
+فال بكتيريا اللى بتتميز بإنه إلها حاجة واضحة من ال
+
+242
+00:18:23,980 --> 00:18:28,380
+growth factors بنسميها fastelias البعض بيطلق عليها
+
+243
+00:18:28,380 --> 00:18:31,920
+البكتيريا المدلة من ناحية الغذائية يعني لها
+
+244
+00:18:31,920 --> 00:18:37,350
+requirement خاصةمن ال growth factors special amino
+
+245
+00:18:37,350 --> 00:18:40,330
+acids معينة بنضيفهم بعض ال purines و بعض ال
+
+246
+00:18:40,330 --> 00:18:43,930
+pyrimidines و بعض الأحيان ال vitamins فى أن ال
+
+247
+00:18:43,930 --> 00:18:50,330
+prosela بتحتاج vitamin B لبروسيلا وبالتالي بنسميها
+
+248
+00:18:50,330 --> 00:18:55,450
+برضه fastidious و غالبا ال fastidious بتكون slow
+
+249
+00:18:55,450 --> 00:19:00,500
+growing ماهي slow growing؟يعني بدل ما تاخد 24 ساعة
+
+250
+00:19:00,500 --> 00:19:04,520
+لمستعمرات حتى تظهر، خد بالك، هنتكلم الآن عن
+
+251
+00:19:04,520 --> 00:19:09,340
+العوامل اللي بتأثر على النمو، صح؟ وبالتالي يعني
+
+252
+00:19:09,340 --> 00:19:14,280
+بعتقد انه في نهاية الموضوع مضطرين نتكلم عن نمو
+
+253
+00:19:14,280 --> 00:19:17,200
+البكيريالأن احنا بتتكلم عن العوامل اللي بتأثر على
+
+254
+00:19:17,200 --> 00:19:20,100
+النمو التالي، احنا بتتكلم عن نمو itself، احنا
+
+255
+00:19:20,100 --> 00:19:25,340
+بننمو بالطول، بنجيس طولنا، ممكن نجيس الوزن تبعنا،
+
+256
+00:19:25,340 --> 00:19:28,300
+طبعا إن احنا زدنا في الوزن، بس نمو البكتيدا لا
+
+257
+00:19:28,300 --> 00:19:34,100
+يقاس بأي من المقاييس هذه، يقاس بالعددنمو البكتيريا
+
+258
+00:19:34,100 --> 00:19:43,480
+يقاس بالعدد 2 4 8 16 32 64 132 وها كده اللي هي
+
+259
+00:19:43,480 --> 00:19:47,580
+اتضاعف لإن الخلية بال C تنتهي تنتهي بال Zero أربعة
+
+260
+00:19:47,580 --> 00:19:52,520
+لإنه إنكسام فنائي بصير تبقى بتعبد الأرقام 2 4 8 16
+
+261
+00:19:52,520 --> 00:19:56,840
+كده تحفظوهم في كل شيء كمان مرة اشتغلش مصطلح اللي
+
+262
+00:19:56,840 --> 00:20:00,970
+باللون الأحمر fastidias ماذا يعني؟يعني أن
+
+263
+00:20:00,970 --> 00:20:03,990
+البكتيريا لها requirement خاصة من ال growth
+
+264
+00:20:03,990 --> 00:20:08,710
+factors نجي لعامل تاني غير ال nutrient اللي هو
+
+265
+00:20:08,710 --> 00:20:13,660
+متعلق بال temperatureأكيد في التلاجة احنا بنحفظ
+
+266
+00:20:13,660 --> 00:20:19,300
+الأغنية ومع ذلك اكتر طلعنا من التلاجة أغنية صار
+
+267
+00:20:19,300 --> 00:20:24,200
+فيها عفن او فيها فساد زي حليب طلعت إله رائحة كريهة
+
+268
+00:20:24,200 --> 00:20:28,680
+أو شفنا بُقع على فاكة أو خضروات في التلاجة هذا
+
+269
+00:20:28,680 --> 00:20:33,180
+مؤشر على إيش؟ على إن المسبب يستطيع إنه في درجات
+
+270
+00:20:33,180 --> 00:20:36,240
+حرارة منخفضة لدرجات حرارة التلاجة هذا قبل حتى
+
+271
+00:20:36,240 --> 00:20:39,120
+موضوع قطع الكهربا، هذا عمر طبيعي إنه يصير في فساد
+
+272
+00:20:39,120 --> 00:20:39,720
+في التلاجة
+
+273
+00:20:42,440 --> 00:20:46,920
+ننتقل لموضوع تاني غير تلاجة، هناك بعض الأطعمة تحفظ
+
+274
+00:20:46,920 --> 00:20:53,740
+ساخنة، كيف تحفظ ساخنة؟ يعني مثلا عندنا اليوم وليمة
+
+275
+00:20:53,740 --> 00:21:00,180
+و حضرنا الطعام الساعة أحداشر اتناشر كان جاهز، لكن
+
+276
+00:21:00,180 --> 00:21:04,570
+الوليمة الساعة خمسة المساءوبالتالي انا مضطر احفظ
+
+277
+00:21:04,570 --> 00:21:08,370
+الغذاء هذا في درجة حرارة خمسين او ستين درجة مقوية
+
+278
+00:21:08,370 --> 00:21:13,010
+عشان يلاله ساخن مايتسخنش برة اخرى لانه بيفقد بعض
+
+279
+00:21:13,010 --> 00:21:16,950
+ال quality السابقة اللي هو تتسخن و برد هناك بعض
+
+280
+00:21:16,950 --> 00:21:20,110
+البكتيريا قد تنمو في هذا الغذاء و هتسبب food
+
+281
+00:21:20,110 --> 00:21:25,310
+poisoningتمام؟ لإن جنافيان دى فى التلاجة مش خالصان
+
+282
+00:21:25,310 --> 00:21:28,570
+وعلى حرارة مرتفعة برضه ايه؟ فى أننا ناس او فينا
+
+283
+00:21:28,570 --> 00:21:31,690
+مجموعات من البكتيريا عندها المقضة عليها أنها تنمو
+
+284
+00:21:31,690 --> 00:21:34,670
+و تماننا نزيزة طب احنا اجسامنا سبعة و تلاتين درجة
+
+285
+00:21:34,670 --> 00:21:38,210
+موية اللى هى عالى جدا وليها مخفضة وبرضه بنصاد
+
+286
+00:21:38,210 --> 00:21:42,960
+الاتهابات للبكتيريا، إذن حاجة البكتيرياللحرارة
+
+287
+00:21:42,960 --> 00:21:46,780
+تختلف من نوع الباتيريا صنفناه من تصنيف بسيط في
+
+288
+00:21:46,780 --> 00:21:50,220
+تصنيفات أعقد من هذه إلى ثالث أصناف Cyclophilic محب
+
+289
+00:21:50,220 --> 00:21:54,360
+للبرودة و Mesophilic محب لدرجة حرارة الوسطى و
+
+290
+00:21:54,360 --> 00:22:00,580
+Thermophilic المحب للهوايا المرتفعة 15-20 طبعا
+
+291
+00:22:00,580 --> 00:22:03,280
+خدوا بالكم انا هتكلم عن المصطلح الآن او تلت
+
+292
+00:22:03,280 --> 00:22:07,320
+مصطلحها احفظوهم كويس او فهموهم كويس الهندية حاسمة
+
+293
+00:22:07,320 --> 00:22:11,080
+minimum و حاسمة maximum و حاسمة optimum
+
+294
+00:22:28,860 --> 00:22:37,590
+أفضل نمو هو كلمة optimumالان واضح انه لما انا عملت
+
+295
+00:22:37,590 --> 00:22:41,030
+إشارة بالأصابيع قلت minimum و maximum و في النص
+
+296
+00:22:41,030 --> 00:22:48,530
+ايه ال optimum لا يعني ان الاصبع والأوسط في النص
+
+297
+00:22:48,530 --> 00:22:51,730
+ان درجة الحرارة ال optimum بتكون بين التنتين قد
+
+298
+00:22:51,730 --> 00:22:55,450
+تكون قريبة لل minimum او قد تكون قريبة ال maximum
+
+299
+00:22:55,450 --> 00:23:00,110
+او قد تكون في المنتصف يعني ليس هنا قانون بيقول ان
+
+300
+00:23:00,110 --> 00:23:04,380
+ال optimum وين يكون موجودقريب من ال .. و هنشوف
+
+301
+00:23:04,380 --> 00:23:07,880
+كمان صورة الآن و نريكوا أن ال optimum من الاشريشيا
+
+302
+00:23:07,880 --> 00:23:13,000
+كولاي قريب من ال maximum يعني مافيش يقولنا قانون
+
+303
+00:23:13,000 --> 00:23:17,900
+بيحكم، بيعتمد على نوع البكي اتفقنا كام صنف على حسب
+
+304
+00:23:17,900 --> 00:23:23,240
+الحرارة؟ cyclophilic, mesophilic, thermophilic و
+
+305
+00:23:23,240 --> 00:23:26,140
+أنا بقولكوا الكتب مليئة بأرقام مختلفة عن الأرقام
+
+306
+00:23:26,140 --> 00:23:31,030
+اللي موجودة هنافخلينا نتظن ب one number او رقم
+
+307
+00:23:31,030 --> 00:23:35,770
+المولود أمامنا الان ال 15-20 مش minimum و maximum
+
+308
+00:23:35,770 --> 00:23:42,290
+الصحيح ال optimum تبعهم من 15-20 لكن قد يكون هناك
+
+309
+00:23:42,290 --> 00:23:44,950
+growth على أربع درجات مقوية التلاجة بتكون من 2 ل8
+
+310
+00:23:44,950 --> 00:23:45,510
+يا تلاجة
+
+311
+00:24:05,870 --> 00:24:09,230
+القائمة اللي انتم مش شايفينها وانا بالكاد استطيع
+
+312
+00:24:09,230 --> 00:24:14,760
+ان اقراهاهيبقى عن مجموعة من أسماء البكتيريا بس بدي
+
+313
+00:24:14,760 --> 00:24:19,560
+أوريكوا أمثلة Listeria monocytogenes ال minimum
+
+314
+00:24:19,560 --> 00:24:24,860
+تبعها one يعني تنمو هنا دلوقت بالتلاجة for sure صح
+
+315
+00:24:24,860 --> 00:24:31,420
+ال maximum خمسة واربعين ال optimum يتراوح من
+
+316
+00:24:31,420 --> 00:24:37,080
+تلاتين لسبعة و تلاتين قريب لل maximum نعمفى هن دى
+
+317
+00:24:37,080 --> 00:24:41,060
+ال vibrio marinus و marinus واضح من اسمها بحرية
+
+318
+00:24:41,060 --> 00:24:46,600
+اربعة برضه بتنمو فى تلاجة بتنمو مش فى تلاجة ال
+
+319
+00:24:46,600 --> 00:24:52,880
+optimum ممكن تدهش طب احنا جولنا ال minimum اربعة
+
+320
+00:24:52,880 --> 00:24:55,920
+هل يعني ذلك انه
+
+321
+00:25:00,100 --> 00:25:02,800
+عشان تلاتة اتنين واحدش هتنمو، ولكن اربعة، خمسة،
+
+322
+00:25:02,800 --> 00:25:06,980
+ستة، واتذكر تلاتة يقولنا من اتنين لتمانية، وبتالي
+
+323
+00:25:06,980 --> 00:25:12,000
+فيه إمكانيلا النموم، صحيح مش النمو optimum عشان
+
+324
+00:25:12,000 --> 00:25:14,900
+هيك اللي بنقول احنا ال low temperature و ال slow
+
+325
+00:25:14,900 --> 00:25:18,960
+down، عشان ال slow down هتبطئ، بدل الطعام مايفسد
+
+326
+00:25:18,960 --> 00:25:23,460
+خلال يومين لو كان ع سبعة و تانية درجة مقوية، بيفسد
+
+327
+00:25:23,460 --> 00:25:25,360
+مثلا بعد أسبوع أو أسبوعين
+
+328
+00:25:31,510 --> 00:25:34,450
+الجدل اللى قدامنا كمان مرة بوضح لي الميكروبات ال
+
+329
+00:25:34,450 --> 00:25:36,610
+minimum و ال maximum طبعا مش هنحفظهم هدول
+
+330
+00:25:36,610 --> 00:25:41,830
+الموجودين في أمامنا على اللوان لكن الشكل اللى
+
+331
+00:25:41,830 --> 00:25:45,330
+قدامكوا هذا شكل كتير كويس بيفهمنا الفكرة اللى بدأت
+
+332
+00:25:45,330 --> 00:25:48,950
+فيها أنا ليها ال minimum و ال maximum هي طبعا
+
+333
+00:25:48,950 --> 00:25:56,190
+درجات الحرارة ال degrees Celsius عندي حوالى 9 او 8
+
+334
+00:25:56,190 --> 00:25:59,850
+درجات مئوية below which Escherichia coli will not
+
+335
+00:25:59,850 --> 00:26:06,620
+growأقل من 8 الإشريشيا كولاي مش هتنمو، أكتر من 48
+
+336
+00:26:06,620 --> 00:26:11,340
+درجة مئوية لن تنمو البكتيريا، لن تنمو الإشريشيا
+
+337
+00:26:11,340 --> 00:26:18,160
+كولاي، وين أفضل نمو؟ على 37، 37 كنا كم مرة أقرب
+
+338
+00:26:18,160 --> 00:26:22,180
+إلى 48 من مين؟ من اللي هو ال minimum، يعني أقرب
+
+339
+00:26:22,180 --> 00:26:27,710
+إلى ال maximum من ال minimumوفي هذه النقطة إشارة
+
+340
+00:26:27,710 --> 00:26:32,930
+واضحة بإنه ال .. ال .. ال .. ال .. ال ..
+
+341
+00:26:32,930 --> 00:26:37,010
+الmicrobial تختلف في حالتها لل temperature
+
+342
+00:26:37,010 --> 00:26:40,250
+وبالتالي بدنا نكون عارفين إن معظم ال medically
+
+343
+00:26:40,250 --> 00:26:43,130
+important bacteria .. medically important bacteria
+
+344
+00:26:43,130 --> 00:26:47,750
+can grow at a mesophilic temperature لإنهم بسببنا
+
+345
+00:26:47,750 --> 00:26:51,970
+أمراض وبالتالي جسمنا 37 و ال optimum بتبعهم بيكون
+
+346
+00:26:51,970 --> 00:26:58,010
+قريب من هذه الدرجةميجي لل pH ال hydronium ion
+
+347
+00:26:58,010 --> 00:27:02,110
+concentration او ال pH زي ما ال temperature عامل
+
+348
+00:27:02,110 --> 00:27:08,310
+حاسم في نمو البكتيريا ايضا هاد اللي عامل حاسم ايضا
+
+349
+00:27:08,310 --> 00:27:13,450
+وقاله minimum وقاله maximum وقاله optimum عارفين
+
+350
+00:27:13,450 --> 00:27:17,430
+ليش؟ مين فيكوا درس clinical chemistry؟
+
+351
+00:27:19,470 --> 00:27:23,930
+مين عمل فحص ال glucose؟ او حد ما وصلوش للفحوصات
+
+352
+00:27:23,930 --> 00:27:30,230
+هذه في المختبأ؟ طيب الإنزيمات .. الإنزيمات احنا
+
+353
+00:27:30,230 --> 00:27:36,000
+بنعملها فحص على pH محددهذا ال pH هو ال optimum
+
+354
+00:27:36,000 --> 00:27:39,220
+معناه الحاجة ان في عندها مرضوية minimum و في عندها
+
+355
+00:27:39,220 --> 00:27:44,540
+maximum below which لذلك لما احنا بنقول ان ال pH
+
+356
+00:27:44,540 --> 00:27:48,620
+غير ملائم هو غالبا غير ملائم لمين ده الإنزيمات
+
+357
+00:27:48,620 --> 00:27:54,760
+اللي موجودة في الخليل هناك بعض أنواع من الميكروبات
+
+358
+00:27:54,760 --> 00:28:00,410
+بتحب ال pH المتعددو في ميكروبات بتحب ال pH المنخفض
+
+359
+00:28:00,410 --> 00:28:03,810
+كلكوا بتعرفوها و بتاكلوها كل يوم بتاكلوها او مش كل
+
+360
+00:28:03,810 --> 00:28:09,630
+يوم البعض منكوا المخللات المخلل المخلل أصلا تصنيعه
+
+361
+00:28:09,630 --> 00:28:15,070
+هو عبارة عن بكتيريا بتخفض ال pH تبع الخضروات اللي
+
+362
+00:28:15,070 --> 00:28:17,790
+بتعملوها المخلل زي الأخيار على سبيل المثال بنزل ال
+
+363
+00:28:17,790 --> 00:28:24,390
+pH تبعه إلى طعم أو إلى مضاق المقبول لديكم الحليب
+
+364
+00:28:24,390 --> 00:28:30,830
+بتحول للبناللبن بيكون حامض، حموضة مقبولة لديكم،
+
+365
+00:28:30,830 --> 00:28:35,410
+لما بنزينش بيسموه حمضمحمد مش معناته الحاجة انه ..
+
+366
+00:28:35,410 --> 00:28:40,010
+انه فاسد، محمد معناته انه درجة الحموضة نزلت ..
+
+367
+00:28:40,010 --> 00:28:43,790
+يعني .. يعني ارتفعت الحموضة من ال beach نزل تمام
+
+368
+00:28:43,790 --> 00:28:48,190
+إلى درجة غير مقبولة ذوقيا، اللي هو الذوق، فلذلك هو
+
+369
+00:28:48,190 --> 00:28:52,490
+ليس فساد صحي وانما فساد ذوقي، بنسميه organoleptic
+
+370
+00:28:52,490 --> 00:28:55,650
+spoilage organoleptic spoilage يعني ان النفس لا
+
+371
+00:28:55,650 --> 00:29:01,810
+تتدوق بيه إيجابية، تتدوق بيه سالمةإذا نعرف أنه في
+
+372
+00:29:01,810 --> 00:29:06,250
+أسيلوفيليك محب للحق لل .. لل pH المقفض في نموذج
+
+373
+00:29:06,250 --> 00:29:11,510
+صارخ طبي لبكتيريا بتحب ال alkaline pH اللي هي ال
+
+374
+00:29:11,510 --> 00:29:16,630
+Vibrio و دبالكوا احنا المعلومات اللي بتدرسها في ال
+
+375
+00:29:16,630 --> 00:29:21,550
+microbiology من باب اعرف عدوك اعرف عدوك لماذا
+
+376
+00:29:21,550 --> 00:29:26,890
+نتعلم عن أعدائنا او عن أعدائنا نتعلم عشان احنا
+
+377
+00:29:26,890 --> 00:29:31,190
+نعرف كيف نتعافل معهمنتعامل معهم سواء كان بإيجارية
+
+378
+00:29:31,190 --> 00:29:35,870
+أو بسلبية، في الآخر نتعامل معهم البكتيريا اللي من
+
+379
+00:29:35,870 --> 00:29:40,810
+نوع Vibrio بتحب ال alkaline pH احنا مسكنا هذه
+
+380
+00:29:40,810 --> 00:29:46,290
+المعلومة، تمام؟ واستفدنا منها في أكثر من طريقة،
+
+381
+00:29:46,290 --> 00:29:52,070
+ناخد الاستفادة الأولى لما أردنا عزل البكتيريا من
+
+382
+00:29:52,070 --> 00:29:56,930
+ال stoolيحتوي لجرام الواحد على عشر أقص اتناش
+
+383
+00:29:56,930 --> 00:30:01,830
+تقريبا بكتيريا لكل جرام و من أنواع مختلفة و بدي
+
+384
+00:30:01,830 --> 00:30:04,310
+أعزل مايكروب واحد اسمه Vibrio من بين كل
+
+385
+00:30:04,310 --> 00:30:08,490
+هالمايكروبات المهولة فبرا بحطهم في وسط ال بي اتش
+
+386
+00:30:08,490 --> 00:30:13,150
+سبعه alkaline كلهم بموتوا إلا ال Vibrio يبقى
+
+387
+00:30:13,150 --> 00:30:16,350
+استفدت من ال .. استفدت من المعلومة بال selective
+
+388
+00:30:16,350 --> 00:30:21,680
+isolation معي ولا مش معي؟استفادت معلومة تانية من
+
+389
+00:30:21,680 --> 00:30:25,060
+ناحية طبية، أنا مش جادر أغلى الميه في .. ماعنديش
+
+390
+00:30:25,060 --> 00:30:28,140
+طاقة أغلى الميه للناس و أقول للناس اغلىوا الميه،
+
+391
+00:30:28,140 --> 00:30:31,160
+بنقولهم بطريقة تانية، بنقولهم ممكن تعصروا لمون على
+
+392
+00:30:31,160 --> 00:30:37,240
+كأس الماء قبل شربه، اللامونش بيعمل، بيخفض ال pH و
+
+393
+00:30:37,240 --> 00:30:43,510
+بيقتل ال .. ال vibrio كلها، بيجعل ظروفها صعبةالقصد
+
+394
+00:30:43,510 --> 00:30:48,450
+من الكلام انه لما بنتعلم المعلومة احنا المعلومة مش
+
+395
+00:30:48,450 --> 00:30:52,850
+فقط للحفظ وانما كيف يمكن استغلال هذه المعلومة
+
+396
+00:30:52,850 --> 00:30:57,130
+تطبيقيا وانا بجيب أمثلة خلال الشرح لكن انا اتمنى
+
+397
+00:30:57,130 --> 00:31:01,130
+ان العقلية تبعتنا العقلية تبعت الطالب تتحول من
+
+398
+00:31:01,130 --> 00:31:06,920
+متلقي معلوماتالى قدرة تحليلية انا كيف ممكن استفيد
+
+399
+00:31:06,920 --> 00:31:10,360
+من هذه المعلومة وانا اعتقد ان مجال ال microbiology
+
+400
+00:31:10,360 --> 00:31:14,480
+من اروع العلوم اللى ممكن الطالب او اللى دانس لي
+
+401
+00:31:14,480 --> 00:31:19,220
+هذا المصاد انه يستفيد من المعلومات بشكل تطبيقى و
+
+402
+00:31:19,220 --> 00:31:24,100
+ايجابى معظم الميكروبات اللى بسببنا امراض هى من
+
+403
+00:31:24,100 --> 00:31:28,300
+النوع الاول اللى بتحب ال ph تبع ال near ال
+
+404
+00:31:28,300 --> 00:31:34,560
+neutrality القريب من اللى هو التعاملبرضه جدول اخر
+
+405
+00:31:34,560 --> 00:31:40,000
+بوضح لنا بعض الميكروبات شوفوا مثلا الميكروب الاول
+
+406
+00:31:40,000 --> 00:31:43,820
+اسمه thiobacillus thioxidans ال minimum pH point
+
+407
+00:31:43,820 --> 00:31:49,720
+five يعني very very very acidic و ال maximum اربعة
+
+408
+00:31:49,720 --> 00:31:53,940
+لستة و ال optimum من اتنين لاثنين و تمانية من عشر
+
+409
+00:31:53,940 --> 00:31:59,760
+في Vibrio عندنا ولا مش Vibrio؟في اندي هنا واحدة من
+
+410
+00:31:59,760 --> 00:32:07,080
+المحبات لل pH ال minimum pH تبعها خمسة الى خمس
+
+411
+00:32:07,080 --> 00:32:12,060
+تمانية ال optimum قريب من ال neutral ال maximum من
+
+412
+00:32:12,060 --> 00:32:17,030
+تمانية و نص الى تسعةفي عندي لورع على طول Erwinia
+
+413
+00:32:17,030 --> 00:32:20,750
+من تسعة و تلاتة، ال maximum، في عندي كمان
+
+414
+00:32:20,750 --> 00:32:25,290
+Thiobacillus تسعة، يعني في عندي تنوع أفوان، تنوع
+
+415
+00:32:25,290 --> 00:32:29,930
+مختلف من الميكروبات، البعض منها .. البعض منها
+
+416
+00:32:29,930 --> 00:32:34,190
+بيكون مفضل ال .. ال .. ال acidic، البعض الأخر يفضل
+
+417
+00:32:34,190 --> 00:32:37,690
+ال .. ال alkaline، لكن كما ذكرتوا لكم معظم ال
+
+418
+00:32:37,690 --> 00:32:40,770
+medical important bacteria they prefer ash اللي هو
+
+419
+00:32:40,770 --> 00:32:48,730
+pH near and neutralنجي للعامل الثالث بعد الرابع
+
+420
+00:32:48,730 --> 00:32:51,790
+اعتقد هذا هو الاول كان ال nutrient التاني كان
+
+421
+00:32:51,790 --> 00:32:53,890
+الحرارة التالت كان البيوتش الان هو ال oxygen
+
+422
+00:32:53,890 --> 00:32:58,870
+required لو افترضنا افترضنا جدلا اي واحد و جالكه
+
+423
+00:32:58,870 --> 00:33:05,120
+ال oxygen سام للبشرطبعا حتى هجوموا على طول انتوا و
+
+424
+00:33:05,120 --> 00:33:10,160
+تقولوا انه انت تتكلم كلام غير منطقي البتة لإن احنا
+
+425
+00:33:10,160 --> 00:33:15,220
+إذا منعنا ال oxygen بنموت صح؟ فكيف لك أن تصف ال
+
+426
+00:33:15,220 --> 00:33:18,660
+oxygen بالمادة السامة للبشر؟ أنا الآن بتوضح حالي
+
+427
+00:33:18,660 --> 00:33:25,920
+الفكرة و أرجو انكوا تكونوا صابورين لفهم الموضوع ال
+
+428
+00:33:25,920 --> 00:33:30,880
+oxygen رمزه O2 صح؟ و O2 يعني ذرة و ذرة من ال
+
+429
+00:33:30,880 --> 00:33:35,300
+oxygen، ذرتين oxygenالوظيفة اللى بيعملها الاكسجين
+
+430
+00:33:35,300 --> 00:33:40,240
+فى داخل خلايانا احنا انه it acts as a final
+
+431
+00:33:40,240 --> 00:33:42,680
+electron acceptor ميش يعني final electron
+
+432
+00:33:42,680 --> 00:33:45,880
+acceptor؟ مستقبل أخي الالكترونات زوج من
+
+433
+00:33:45,880 --> 00:33:48,900
+الالكترونات بتطلع من مركب لمركب لمركب لمركب وفي
+
+434
+00:33:48,900 --> 00:33:52,740
+الآخر لازم واحد يحمله مين اللى بيحمله؟ الاكسجين
+
+435
+00:33:52,740 --> 00:33:58,800
+وإن هذه العملية عملية صعبة شوية قد تقسم ظهر
+
+436
+00:33:58,800 --> 00:34:06,490
+الاكسجينهي تشطر فيتحول الى O و O بنسميها O singlet
+
+437
+00:34:06,490 --> 00:34:12,350
+singlet واحدة طبيعة الأكسجين مابقدرش يعيش singlet
+
+438
+00:34:12,350 --> 00:34:19,490
+في بعض الإنزيمات في الخلية أطول بتشوف مش بعض
+
+439
+00:34:19,490 --> 00:34:23,910
+الإنزيمات حتى لحاله بدون إنزيمات ال O مع ال H2O
+
+440
+00:34:23,910 --> 00:34:28,470
+بيكونوله H2O2 hydrogen peroxide ال hydrogen
+
+441
+00:34:28,470 --> 00:34:30,110
+peroxide يوصف
+
+442
+00:34:34,160 --> 00:34:38,280
+Powerful Oxidizing agent يعني هيدمر بعض
+
+443
+00:34:38,280 --> 00:34:41,600
+الموليكيولز في الخلية زي ال DNA زي ال RNA زي ال
+
+444
+00:34:41,600 --> 00:34:43,460
+protein زي ال carbohydrate اللي هي بيسموها ال
+
+445
+00:34:43,460 --> 00:34:48,420
+macro molecules الجزيئات الكبيرة ال O التانية
+
+446
+00:34:48,420 --> 00:34:52,260
+وراها ال O التانية قد تتحول إلى ما يسمى super
+
+447
+00:34:52,260 --> 00:34:58,150
+oxideيبقى في اندي أو تفاعل مع هيدروجين يعفر مع ماء
+
+448
+00:34:58,150 --> 00:35:02,730
+وتكون H2O2 و واحدة تانية اتحولت إلى ما يسمى بال
+
+449
+00:35:02,730 --> 00:35:06,070
+super oxide و كلا هما ال hydrogen peroxide و ال
+
+450
+00:35:06,070 --> 00:35:09,250
+super oxide كلا هما very powerful oxidizing agent
+
+451
+00:35:09,250 --> 00:35:16,230
+بيقتل الخلايا إذا احنا ميتين احنا ميتين بالطريقة
+
+452
+00:35:16,230 --> 00:35:22,850
+هذه طب ليش احنا مش ميتين؟لأن الجسم بتاعنا في
+
+453
+00:35:22,850 --> 00:35:27,630
+system بيحمينا من ال oxygen toxicity كل ما يتكون
+
+454
+00:35:27,630 --> 00:35:32,370
+super oxide بيعارب تأثيره هذا ال انزين كل ما يتكون
+
+455
+00:35:32,370 --> 00:35:35,970
+hydrogen peroxide بيحلله هذا ال انزين هنشوف هذه
+
+456
+00:35:35,970 --> 00:35:42,650
+الانزيمات بعد قليل لكن بناناعرف أن ال oxygen سام
+
+457
+00:35:43,850 --> 00:35:47,570
+الوكسجين ساعة هذا المفهوم الجديد بالنسبالكوا ارجو
+
+458
+00:35:47,570 --> 00:35:52,170
+انكوا تكونوا افهمتوا ليش احنا وصلنا لهذا المفهوم
+
+459
+00:35:52,170 --> 00:35:55,070
+اللوحة اللي امامكوا ليس لها علاقة بالمفهوم اللي
+
+460
+00:35:55,070 --> 00:36:01,830
+حكيته لكن كويس الان نعرض اللوحة و نشوف الشكل
+
+461
+00:36:01,830 --> 00:36:06,970
+اللي امامكوا ايش بيعمل لو انا حطيت بكتيريا في
+
+462
+00:36:06,970 --> 00:36:12,460
+انبوبةبكتيريا رجهولة قد تتخذ واحد من الخمس أشكال
+
+463
+00:36:12,460 --> 00:36:17,740
+اللي أمامكم رقم واحد البكتيريا و النامية على الصدر
+
+464
+00:36:17,740 --> 00:36:23,240
+البكتيريا رقم اتنين و النامية بالقعب الأولى هوائية
+
+465
+00:36:23,240 --> 00:36:29,000
+إجباريةوالتانية لا هوائية إشبالية ليش أنا استنتجت
+
+466
+00:36:29,000 --> 00:36:33,000
+هذا الاستنتاج لأنه الأنبوبة بشكل عام أي عمود من
+
+467
+00:36:33,000 --> 00:36:36,420
+الماء لو جينا نطلع على مستوى ال oxygen فيه كل ما
+
+468
+00:36:36,420 --> 00:36:39,900
+نزلنا تحت كل ما قال مستوى ال oxygen كل ما طلعنا
+
+469
+00:36:39,900 --> 00:36:44,140
+فوق كل ما زاد مستوى ال oxygen ففوق بالفعل في رقم
+
+470
+00:36:44,140 --> 00:36:49,040
+واحد في oxygen نبت البكتيريا تحت فيش oxygen نبت
+
+471
+00:36:49,040 --> 00:36:52,920
+البكتيريا فهذه بكتيريا لا هوائيةلحظوا مانا ماتش
+
+472
+00:36:52,920 --> 00:36:57,460
+فوق لإنه فيه Oxygen إيه صار فيها؟ ماتت البكتيريا،
+
+473
+00:36:57,460 --> 00:37:01,980
+اللي فوق ماتت، ماتت لإن ال Oxygen سام بالنسبيلها،
+
+474
+00:37:01,980 --> 00:37:06,260
+زي ما احنا سام بالنسبيلنا، بس الفرق بيننا وبينها
+
+475
+00:37:06,260 --> 00:37:11,000
+إنه هي لاتمتلك ما نمتلك نحن من إنزيمات تعادل صمية
+
+476
+00:37:11,000 --> 00:37:15,480
+الواء فبتوقع البكتيريا الهوائية يكون نفس الأنظمة
+
+477
+00:37:15,480 --> 00:37:18,100
+اللي عندنا موجودة عندها، بينما البكتيريا اللي
+
+478
+00:37:18,100 --> 00:37:22,360
+هوائية ماعنداش هذه الأنظمةرقم تلاتة، والله
+
+479
+00:37:22,360 --> 00:37:24,820
+البكتيري هذا اللي فوق، التلاتة من ما هي فارقة
+
+480
+00:37:24,820 --> 00:37:29,400
+معاها صح؟ لأ مش فارقة معاها، نامية فوق و نامية
+
+481
+00:37:29,400 --> 00:37:37,060
+تحت، هتكلم عنها بعد قليل، رقم أربع، لأ و النامية،
+
+482
+00:37:37,060 --> 00:37:41,550
+على السطح ولا تحت السطح شوية؟هذه بكتيريا بتحب تشيل
+
+483
+00:37:41,550 --> 00:37:44,630
+أكسجين بكميات كبيرة، بتحب بكميات قليلة، بيسموها
+
+484
+00:37:44,630 --> 00:37:50,330
+micro-aerophilic يعني ترغب بكميات أكسجين
+
+485
+00:37:50,330 --> 00:37:53,990
+مُنقَفَرة، نسبة الأكسجين في الجو اللي جاتاش يا
+
+486
+00:37:53,990 --> 00:37:57,970
+فنات، يعني في الجو العادي، في البيئة اللي احنا
+
+487
+00:37:57,970 --> 00:38:02,110
+عايشين فيها، جاتاش نسبة الأكسجين؟ عشرين في المين،
+
+488
+00:38:02,110 --> 00:38:06,330
+تقريبًا عشرين تسعة من عشر، يعني تقريبًا واحد عشرين
+
+489
+00:38:06,330 --> 00:38:10,880
+بالميةالبكتيرى الهوائية العادية اللى في رقم واحد
+
+490
+00:38:10,880 --> 00:38:16,320
+بتعيش عندها المستوى بدون اي مشاكل رقم اربع فى
+
+491
+00:38:16,320 --> 00:38:19,800
+اللوحة اللى هى ال microaerophilic فقط بده خمسة
+
+492
+00:38:19,800 --> 00:38:24,040
+فالمية فلاحظوا الخمسة فالمية وانا حصلتهم مش على
+
+493
+00:38:24,040 --> 00:38:29,070
+السطح مباشرة من تحت السطحرقم خمسة و رقم تلاتة إيش
+
+494
+00:38:29,070 --> 00:38:31,870
+الفرق بينهم؟ مين يرفع أيه اللي بيقول إيش الفرق بين
+
+495
+00:38:31,870 --> 00:38:35,890
+التنان؟ تلاتة هي تلاتة ما هي هوائية اغتيارية لإنه
+
+496
+00:38:35,890 --> 00:38:39,330
+نموها في كدة يوم بس فوق كان تركيزها أكتر عن
+
+497
+00:38:39,330 --> 00:38:43,830
+الأكسوجين لكن هناك لا هوائية اغتيارية يعني إنه كان
+
+498
+00:38:43,830 --> 00:38:47,470
+تنانيه في كل النموبة بس يعني إنه نموها فاقش كتير
+
+499
+00:38:47,470 --> 00:38:52,610
+كيف لا هوائية اغتيارية يعني؟ بتعيش في ظروف مافيهاش
+
+500
+00:38:52,610 --> 00:38:55,490
+.. فيها أكسوجين و مافيهاش بس بتفضل اللي مافيهاش
+
+501
+00:38:55,490 --> 00:38:59,430
+اغتياريةرقم تلاتة رقم تلاتة facultative أنا
+
+502
+00:38:59,430 --> 00:39:02,030
+aerobic و كلامك دقيق مائة بالمائة facultative أنا
+
+503
+00:39:02,030 --> 00:39:06,610
+aerobic لإنه نمت فوق و نمت تحت لكن نموها في الأعلى
+
+504
+00:39:06,610 --> 00:39:11,610
+أكتر لإن أنا أفضل aerobic رقم خمسة لا يطلق عليها
+
+505
+00:39:11,610 --> 00:39:17,760
+اللقى المصطلح اللي أطلقتيه انت شايف ايه المصطلح؟لأ
+
+506
+00:39:17,760 --> 00:39:22,760
+يطلق عليها لهوائية تتحمل ولود الأكسجين بمعنى إنه
+
+507
+00:39:22,760 --> 00:39:29,640
+هي أصلا لهوائية لكن فوّت قالية لتحمل الأكسجين فهو
+
+508
+00:39:29,640 --> 00:39:33,840
+بالتالي نمت في كل الإنقوبة بنفس المستوى، خدوا
+
+509
+00:39:33,840 --> 00:39:38,360
+بالكوا، تحمل الشيء غير استخدام الشيء، يعني ما
+
+510
+00:39:38,360 --> 00:39:44,620
+استخدمت؟ الأكسجين، بينما رقم تلاتة فوجها ناشع عملت
+
+511
+00:39:45,920 --> 00:39:51,120
+استخدام ال oxygen، كمان مرة، خمس أنواع، انسوا
+
+512
+00:39:51,120 --> 00:39:54,800
+اللوحة، انسوا اللوحة، تعالوا نحفظهم على قاعد
+
+513
+00:39:54,800 --> 00:39:59,520
+الإصبع أو الأصابع، هوائية إجبارية، لا هوائية
+
+514
+00:39:59,520 --> 00:40:03,040
+إجبارية، في المنتصف facultative and European، ده
+
+515
+00:40:03,040 --> 00:40:08,570
+هي بحلقة وصل من الهوائية واللا هوائيةمش الأرهانة
+
+516
+00:40:08,570 --> 00:40:13,090
+هذه micro aerophilic هوائية لكن بدها كمية oxygen
+
+517
+00:40:13,090 --> 00:40:17,870
+قليلة الرابع اللي قريب من اللي لا هوائية بقولنا لا
+
+518
+00:40:17,870 --> 00:40:22,130
+هوائية وسميناها aerotolerant anaerobic يعني لا
+
+519
+00:40:22,130 --> 00:40:26,730
+هوائية ولكن تتحمل وجود ال oxygen فسنعمل خمسة ان
+
+520
+00:40:26,730 --> 00:40:31,750
+شاء الله التالتة
+
+521
+00:40:31,750 --> 00:40:37,830
+هي بكتيريا هوائية بكتيريا هوائيةماذا يعني تالتة
+
+522
+00:40:37,830 --> 00:40:41,370
+البكتيريا الهوائية؟ تستطيع أن تعيش في ورود الهواء
+
+523
+00:40:41,370 --> 00:40:45,870
+و عدم ورود الهواء تستغل الأكسجين و تستغل مراكبات
+
+524
+00:40:45,870 --> 00:40:50,750
+أخرى في التنفس، تمام؟ لكن خدوا بالكم من شغل، لو
+
+525
+00:40:50,750 --> 00:40:56,670
+أنا معاه جزئي جلوكوز و أريد أن أعمله oxidation أو
+
+526
+00:40:56,670 --> 00:41:00,170
+metabolism، متابوليزم دعوني أقول مصطلح أدق، لو
+
+527
+00:41:00,170 --> 00:41:04,870
+عملت له متابوليزم في ورود الأكسجين، سأحصل على 36
+
+528
+00:41:04,870 --> 00:41:10,640
+ATPلو عملتله metabolism في غياب ال oxygen أحسنها
+
+529
+00:41:10,640 --> 00:41:17,260
+اتنين اتنين يبقى فعالية الطاقة الناتجة عن جزء
+
+530
+00:41:17,260 --> 00:41:20,920
+الجلوكوز في وجود ال oxygen بتكون ايه أفضل لذلك
+
+531
+00:41:20,920 --> 00:41:24,920
+الفاكولتات في أنا إيروف بتفضل النمو الهوائي عشان
+
+532
+00:41:24,920 --> 00:41:30,040
+انا لاجيناها فوق أعدادها أكبر من وين من تحت بينما
+
+533
+00:41:30,040 --> 00:41:33,560
+ال air tolerant bacteria قلنالكوامُمطَبَب النمو
+
+534
+00:41:33,560 --> 00:41:39,960
+فيها لإنه ليس الأكسجين دخل في النمو، ليس الأكسجين
+
+535
+00:41:39,960 --> 00:41:44,180
+أي دخل في النمو وإنما البكتيريا بطبيعتها لا
+
+536
+00:41:44,180 --> 00:41:48,220
+هوائية، لا تستخدم الأكسجين ولكن طورت قالية لحماية
+
+537
+00:41:48,220 --> 00:41:55,060
+نفسها من الأكسجين هذا اللوحة اللي أمامكم هي نفس
+
+538
+00:41:55,060 --> 00:42:01,000
+اللوحة اللي شفتوها بس بالكلام مش بالرسمأنا حكيت عن
+
+539
+00:42:01,000 --> 00:42:04,560
+الـ Oxygen toxicity concept اللي هي بتكلم عن ال
+
+540
+00:42:04,560 --> 00:42:09,420
+counter parts اللي هم ال systems الأنظمة اللي
+
+541
+00:42:09,420 --> 00:42:16,060
+استخدمت في التخلص من ال toxicity نتطلع على الدوحة
+
+542
+00:42:16,060 --> 00:42:23,790
+اللي قدامنا هنا أول معادلة بتقول 2O2 زي 2Hتو
+
+543
+00:42:23,790 --> 00:42:30,530
+باستخدام الانزيم اللي اسمه super oxide dismutase
+
+544
+00:42:30,530 --> 00:42:37,510
+SOD super oxide dismutase اتحولت الى H2O2 زائد O2
+
+545
+00:42:37,510 --> 00:42:43,310
+لاحظوا شوية الـ2O2 ها دى اللى فوقيها شحطة هفير
+
+546
+00:42:43,310 --> 00:42:50,580
+شحطة ها دى صغيرة هذا المركب يسمى super oxideSuper
+
+547
+00:42:50,580 --> 00:42:55,100
+Oxide هذا الإشارة اللي عليه تسمى أو تعطي لقب Super
+
+548
+00:42:55,100 --> 00:43:00,240
+Oxide طيب ال Super Oxide Dismutase حول ال Super
+
+549
+00:43:00,240 --> 00:43:06,940
+Oxide إلى Hydrogen Peroxide من سم إلى سم، صح؟ هذه
+
+550
+00:43:06,940 --> 00:43:12,660
+خطوة على الطريقالخطوة التانية ال hydrogen peroxide
+
+551
+00:43:12,660 --> 00:43:16,460
+اللي اتكوّن سواء من ال super oxide بسم تايز او
+
+552
+00:43:16,460 --> 00:43:20,660
+اللي اتكوّن لحاله الاسلام دي hydrogen peroxide
+
+553
+00:43:20,660 --> 00:43:25,400
+انزيم الكتاليزا على طول بياخده و بيحوله إلى رزقنا
+
+554
+00:43:25,400 --> 00:43:30,940
+زائد أكسجين يبقى رجعنا الأكسجين زي ما كان تخلصنا
+
+555
+00:43:30,940 --> 00:43:34,560
+من مين الآن من السمية تبع هذا الأكسجين
+
+556
+00:43:37,090 --> 00:43:41,810
+طيب في عندي معادلة تالتة ايش دخلها المعادلة؟ و لو
+
+557
+00:43:41,810 --> 00:43:47,570
+التاني خلصه؟ التالتة ايش دخلها دي؟ خدوا بالكم هناك
+
+558
+00:43:47,570 --> 00:43:51,570
+بعض المخلوقات لا تحتوي على كتاليز تحتوي على حاجة
+
+559
+00:43:51,570 --> 00:43:56,130
+اسمها peroxidase ال peroxidase بيقوم بنفس العمل
+
+560
+00:43:56,130 --> 00:43:59,010
+اللي بيقوم بيها الكتاليز لكن بطريقة مختلفة بطريقة
+
+561
+00:43:59,010 --> 00:44:04,490
+مختلفة أعطيكم مثال هنا فلو أنا اشتري عندي هنا اتو
+
+562
+00:44:04,490 --> 00:44:12,220
+في المعادلةهنا ال H2O2 بيعطيها لمركّب لمركّب مختزل
+
+563
+00:44:12,220 --> 00:44:18,820
+مركّب مختزل بوجود البروكسدايز بطلع مي زي المركّب
+
+564
+00:44:18,820 --> 00:44:23,420
+مؤكسل رحمة
+
+565
+00:44:23,420 --> 00:44:27,280
+الهيدروجينراح منه الهيدروجين فصار لنا إيه؟ مؤكسد
+
+566
+00:44:27,280 --> 00:44:33,480
+لكن ما طلعش Oxygen بمعنى أخر المحصلة النهائية ال
+
+567
+00:44:33,480 --> 00:44:37,680
+hydrogen peroxide فى التانية اتحول لمين؟ لماء فى
+
+568
+00:44:37,680 --> 00:44:43,100
+الأولى اتحول لماء زاد Oxygen و فى التالى اتحول ماء
+
+569
+00:44:43,100 --> 00:44:48,960
+إلى مركب مؤكسد بجكال مختصر و اتأكسد فى الحالتين
+
+570
+00:44:48,960 --> 00:44:55,330
+خلصنا من صميم ال hydrogen peroxideطبعا هذا نقطة
+
+571
+00:44:55,330 --> 00:44:59,130
+يجب انه نجد في عينها دقيقة واحدة عشان نقولكوا إيش
+
+572
+00:44:59,130 --> 00:45:04,210
+ممكن أستفيد منها تتذكروا ال gram-bostev-cocci؟
+
+573
+00:45:04,210 --> 00:45:08,450
+gram-bostev بكتيريا لما تكون البكتيريا شكلًا
+
+574
+00:45:08,450 --> 00:45:13,790
+كوكساي بنقول gram-bostev كوكساي كروي يعني من أشهر
+
+575
+00:45:13,790 --> 00:45:18,130
+الأسماء اللي هتعرفوها أو عرفتوها is staphylococcus
+
+576
+00:45:18,130 --> 00:45:22,940
+aureusو من أشهر الميكروفات اللي انتوا مرضتوا فيها
+
+577
+00:45:22,940 --> 00:45:27,860
+في يوم من الأيام باللواز، Pencilitis اسمها
+
+578
+00:45:27,860 --> 00:45:33,300
+streptococcus,adenostepilococcus و streptococcus
+
+579
+00:45:33,300 --> 00:45:37,880
+التنتين اجرام بوسط الكوكساية، أحد أهم الفحوصات
+
+580
+00:45:37,880 --> 00:45:43,770
+المميزة بين التنتين فحص الكتالينالستافيلوكوكس
+
+581
+00:45:43,770 --> 00:45:49,890
+كتاليز positive بتطلع كتاليز بينما الستريبتوكوكس
+
+582
+00:45:49,890 --> 00:45:55,190
+كتاليز negative تعاولوا كيف نعمل فحص لكي تكونوا
+
+583
+00:45:55,190 --> 00:46:00,030
+عارفين أن كل معلومة بندرسها هنا لها تطبيق في
+
+584
+00:46:00,030 --> 00:46:04,770
+المعمل هاي شريحة بنجيب قطرة من ال hydrogen
+
+585
+00:46:04,770 --> 00:46:10,250
+peroxide بنحط على القطرة البكتيرى اللي بنفحصها
+
+586
+00:46:11,160 --> 00:46:16,760
+القادة البكتيريا إذا فيها كتاليز هيتفاعل مع ال
+
+587
+00:46:16,760 --> 00:46:19,720
+hydrogen peroxide و يحوله بالمعادلة اللي بالدم و
+
+588
+00:46:19,720 --> 00:46:24,640
+في الوسط هنا إلى ماء زاد مين؟ أكسيجين، الأكسيجين
+
+589
+00:46:24,640 --> 00:46:28,440
+بيطلع من وين؟ بيطلع من مي جاعد اللي هو ال hydrogen
+
+590
+00:46:28,440 --> 00:46:34,640
+peroxide، فهيطلع بشكل مين؟ بشكل فقعةالفحص positive
+
+591
+00:46:34,640 --> 00:46:37,480
+إذا كان تكوّن في قاعك، الفحص negative إذا لم
+
+592
+00:46:37,480 --> 00:46:41,380
+يتكوّن في قاعك، تفهمين إيه السهل الفحص؟ one minute
+
+593
+00:46:41,380 --> 00:46:47,820
+test لكن .. لكن وظائفه وأهميته كبيرة جدا، ميزة بين
+
+594
+00:46:47,820 --> 00:46:51,180
+أستاذ فليك وكاي وبين أستاذ فليك وكاي، وطبعا
+
+595
+00:46:51,180 --> 00:46:55,540
+التفاصيل الأخرى هتعلموها بن وعمل إن شاء الله طيب
+
+596
+00:46:55,540 --> 00:47:00,260
+هنا يعني ..بالنقطة الأخيرة غطينا العوامل اللى
+
+597
+00:47:00,260 --> 00:47:06,340
+بتأثر على نمو البكتيريا البكتيريا تنقسم أو تتكاثر
+
+598
+00:47:06,340 --> 00:47:09,760
+بال simple binary region شوفوا الشكل اللى قدامكوا
+
+599
+00:47:09,760 --> 00:47:14,700
+هذاالمادة الوراثية في البكتيريا اللي هو الكروموزوم
+
+600
+00:47:14,700 --> 00:47:20,640
+صح انقسمت في البداية وكل واحد أخد طرف كل كروموزوم
+
+601
+00:47:20,640 --> 00:47:26,280
+أخد طرف وبدأ يتكون سبتا سبتا حاجز أو septum تمام و
+
+602
+00:47:26,280 --> 00:47:32,080
+بدأ يتكون حاجز الحاجز اجتمل و بعدين صار في انقسام
+
+603
+00:47:32,080 --> 00:47:39,260
+خلوي تعمل مين الأم اللي ولدت؟ اي واحدة منهم؟ هاد
+
+604
+00:47:39,260 --> 00:47:40,280
+الأم ولا هاد الأم؟
+
+605
+00:47:45,440 --> 00:47:51,080
+two daughter cells يعني كل واحدة منهم ماخدة من
+
+606
+00:47:51,080 --> 00:47:55,040
+القديم و ماخدة منها من الجديد two daughter cells
+
+607
+00:47:55,040 --> 00:48:00,080
+هذا انقسام هذه الصورة بتوضح لنا يعني بطريقة رائعة
+
+608
+00:48:00,080 --> 00:48:05,500
+جدا ان كيف ال chromosome بدأ يتجري إلى طرف من
+
+609
+00:48:05,500 --> 00:48:09,840
+طرفين الخلية و هذه المنطقة إذا شفتوا هذه ال cross
+
+610
+00:48:09,840 --> 00:48:13,420
+wall او ال septum بدأت تظهر في المنطقة هذه في
+
+611
+00:48:13,420 --> 00:48:14,380
+المنطقة هذه
+
+612
+00:48:17,080 --> 00:48:20,960
+إذا ما هي وسيلة الانقسام هي ال simple binary بجنس
+
+613
+00:48:20,960 --> 00:48:24,140
+وقلنا ببساطة إن ال chromosome بينقسم ويروح على
+
+614
+00:48:24,140 --> 00:48:26,800
+الجهة وعلى الجهة وبعدين يتكون حاجز طبعا بعض
+
+615
+00:48:26,800 --> 00:48:29,940
+البكتيريا بيبقوا متسقة ب بعضها okay بتنقسم لكن
+
+616
+00:48:29,940 --> 00:48:34,840
+بيبقوا متسقة والانقسام أو عدم الانتصاق اللي بيحدده
+
+617
+00:48:34,840 --> 00:48:38,920
+ال genetic material اللي موجودة في الخلية شوفتم
+
+618
+00:48:38,920 --> 00:48:39,700
+مرة دبلو كوكاي
+
+619
+00:48:49,520 --> 00:48:55,340
+الزمن اللازم للانقسام أو للتضاعف يسمى generation
+
+620
+00:48:55,340 --> 00:48:58,340
+time أو doubling time
+
+621
+00:49:07,960 --> 00:49:11,120
+ماني كومون بكتيريا ال generation time تبقى من
+
+622
+00:49:11,120 --> 00:49:17,900
+عشرين إلى ستين دقيقة تخيلوا بنات في الساعة تنقسم
+
+623
+00:49:17,900 --> 00:49:24,080
+البكتيريا تلت مرة طيب بدى ابيع سيارتي انا اليوم
+
+624
+00:49:24,080 --> 00:49:29,480
+مين اشتريه اعطيكوا
+
+625
+00:49:29,480 --> 00:49:35,020
+عرض تبع السيارةبدي أخد في اليوم الأول منكوا شاكل
+
+626
+00:49:35,020 --> 00:49:38,140
+اللي بتشتريه هبدأ أعطيني شاكل و اليوم التاني بس
+
+627
+00:49:38,140 --> 00:49:44,440
+يضعف النبلة لإتنين شاكل و تلاتين يوم تلاتين يوم و
+
+628
+00:49:44,440 --> 00:49:47,340
+تصوّع أنه في اليوم الأول بدي أخد شاكل و التاني يوم
+
+629
+00:49:47,340 --> 00:49:51,560
+بدي أخد كمان اتنين تلت يوم أربعة لتلاتين يوم مين
+
+630
+00:49:51,560 --> 00:49:57,000
+يشتري اتفقنا بدأت اشتري .. خلاص اتفقنا على المبلة
+
+631
+00:49:58,230 --> 00:50:02,370
+راتبك من هنا لخمسين سنة ان شاء الله بت .. بت ..
+
+632
+00:50:02,370 --> 00:50:07,310
+لإنه احسبوا الرقم هتلاقوه رقم مهول جدا بقى اتنين
+
+633
+00:50:07,310 --> 00:50:10,390
+اص تلاتين اتنين اص تلاتين اللي مع أقل حسبة ممكن
+
+634
+00:50:10,390 --> 00:50:13,850
+تتطلع عليه و تشوف اتنين اص تلاتين كدهش بيطلع الرقم
+
+635
+00:50:13,850 --> 00:50:19,230
+البكتيريا بتتضاع في الساعة الواحدة بعض نوع من
+
+636
+00:50:19,230 --> 00:50:24,190
+البكتيريا تلت مرات فخلال اربع وعشرين ساعة اربع
+
+637
+00:50:24,190 --> 00:50:29,420
+وعشرين في تلاتة اتنين وسبعين72 مرة حطوا 2 قص 72
+
+638
+00:50:29,420 --> 00:50:33,080
+شوفوا الرقم اللي بيطلع مليون .. بلايين من ال micro
+
+639
+00:50:33,080 --> 00:50:35,860
+organism وبالتالي احنا لما نزرع البكتيريا على
+
+640
+00:50:35,860 --> 00:50:40,220
+الطبق نزرع خلية واحدة غريبة نزرع خلية واحدة بعد 24
+
+641
+00:50:40,220 --> 00:50:44,420
+ساعة بنصير نشوف المستعمرات طب البكتيريا حجمها واحد
+
+642
+00:50:44,420 --> 00:50:47,720
+مايكرونعشان انا اشوف العادات بتكون بالملي مترات
+
+643
+00:50:47,720 --> 00:50:50,780
+تمام؟ فعشان اساسي الملي مترات لازم تكون العادات
+
+644
+00:50:50,780 --> 00:50:56,520
+بالبلايين بالبلايين تمام؟ فواضح انه زمن الانقسام
+
+645
+00:50:56,520 --> 00:51:00,400
+في البكتيريا طبعا بتأثر بعوامل كثيرة فاكرين الظروف
+
+646
+00:51:00,400 --> 00:51:03,180
+اللي حكينا؟ لو ان ال temperature عشرة او ال
+
+647
+00:51:03,180 --> 00:51:05,740
+temperature خمستاشر اعتقد ال generation time
+
+648
+00:51:05,740 --> 00:51:10,840
+هيختلفلأنه بيأثر على مين؟ على النمو مش معقول أقول
+
+649
+00:51:10,840 --> 00:51:13,280
+generation time ل minimum و generation time ل
+
+650
+00:51:13,280 --> 00:51:17,380
+maximum ال generation time غالبا بنقول ل مين؟ لل
+
+651
+00:51:17,380 --> 00:51:22,340
+optimum يعني أفضل الظروف بنوفرها للبكتيريا و بنحسب
+
+652
+00:51:22,340 --> 00:51:26,400
+ال generation time و أقول لكم أنه في بعض أنواع من
+
+653
+00:51:26,400 --> 00:51:30,140
+البكتيريا بنختمها للمعلومة ال generation time قد
+
+654
+00:51:30,140 --> 00:51:34,120
+يصل إلى فقط عشر دقائق و نكمل المرة القادمة
+
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/RZakKu7Qfl4_raw.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/RZakKu7Qfl4_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..e44c2c62b1d8cc975fc6cf0daaa283ad7f0c0dcc
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/RZakKu7Qfl4_raw.srt
@@ -0,0 +1,2452 @@
+1
+00:00:20,650 --> 00:00:24,270
+بسم الله الرحمن الرحيم نكمل موضوع ال path genesis
+
+2
+00:00:24,270 --> 00:00:29,570
+للبكتيريا و اليوم هتكلم عن جزء مهم جدا المتعلق بال
+
+3
+00:00:29,570 --> 00:00:33,550
+invasion هذا الجزء يأتي بعد ال virulence ال factor
+
+4
+00:00:33,550 --> 00:00:36,010
+الأساسي اللي درسناه اللي هو ال attachment أو ال
+
+5
+00:00:36,010 --> 00:00:41,070
+adherence و درسنا مجموعة من العوامل تحت هذا ال
+
+6
+00:00:41,070 --> 00:00:43,470
+factor و اليوم هتكلم ان شاء الله عن مجموعة من ال
+
+7
+00:00:43,470 --> 00:00:46,890
+factors اللي بتساهم في ال invasion للبكتيريا
+
+8
+00:00:48,480 --> 00:00:52,320
+أنا ذكرت مثالين الماضية في نهاية المحاضرة مثال
+
+9
+00:00:52,320 --> 00:00:57,620
+السلمون اللي بتاعي فيه وكنا بتدخل عبر الأمعاء عبر
+
+10
+00:00:57,620 --> 00:01:01,660
+الفم إلى الأمعاء و بتختلق جدار الأمعاء عبر ال
+
+11
+00:01:01,660 --> 00:01:06,560
+lymph nodes و بتروح على الدم و بتنتشر من الدم إلى
+
+12
+00:01:06,560 --> 00:01:10,680
+organs متنوعة و مختلفة و سميناها invasive
+
+13
+00:01:10,680 --> 00:01:15,300
+microorganism بينما تحدثنا بالمثال مقابل التيتانوس
+
+14
+00:01:15,300 --> 00:01:19,850
+و كنا تبقىفي نفس المكان اللي .. اللي .. اللي عملت
+
+15
+00:01:19,850 --> 00:01:22,770
+فيه infection بينما ال toxin تبعها هو اللي بينتقل
+
+16
+00:01:22,770 --> 00:01:29,190
+الى اماكن متنوعة فالان احنا هنتطرق لبعض العوامل و
+
+17
+00:01:29,190 --> 00:01:33,470
+اعتقد انه طرقنا اليها بمرور سريع نحاول المرايا مرة
+
+18
+00:01:33,470 --> 00:01:37,850
+أخرى قبل ما نبدأ في محاضرتنا ان شاء اللهلجينا ان
+
+19
+00:01:37,850 --> 00:01:42,670
+في مجموعة كبيرة من ال invasive factors هم عبارة عن
+
+20
+00:01:42,670 --> 00:01:48,870
+انزايم زي ال helionidase زي ال collagenase زي ال
+
+21
+00:01:48,870 --> 00:01:53,410
+coagulase زي اللي هو ال .. ال .. ال kinases و ال
+
+22
+00:01:53,410 --> 00:01:58,160
+neuraminidasesو ال phospholipases و ال licitinases
+
+23
+00:01:58,160 --> 00:02:03,120
+مظبوط ان هما ليسوا عبارة عن انزايم و هذه الانزايم
+
+24
+00:02:03,120 --> 00:02:07,340
+لها خطوة محددة او specific step تملة مثلا ال
+
+25
+00:02:07,340 --> 00:02:09,740
+hyaluronidase بيفك ال hyaluronic acid و ال
+
+26
+00:02:09,740 --> 00:02:12,560
+hyaluronic acid هو اللي مسئول عن ربط ال connective
+
+27
+00:02:12,560 --> 00:02:17,740
+tissue البعض البعض ال collagenases اللي برضه
+
+28
+00:02:17,740 --> 00:02:21,160
+بتفكفك الكولاجين اللي برضه بربط ال muscles ببعضهم
+
+29
+00:02:21,160 --> 00:02:25,150
+ببعضلما نفكر في خلية عن بعضها البعض، إيه اللي
+
+30
+00:02:25,150 --> 00:02:30,590
+بيصير؟ مثلا عندي فقدان .. مثلا عندي فقدان للارتباط
+
+31
+00:02:30,590 --> 00:02:33,390
+بين خلية وخلية وبالتالي بيصير في عندي ال block
+
+32
+00:02:33,390 --> 00:02:38,490
+supply لهذه الخلاية بينقطع وبالتالي في عندي death
+
+33
+00:02:38,490 --> 00:02:43,140
+لهذه الخلايةالكواريو ليه اصفقنا انه بيعمل ال
+
+34
+00:02:43,140 --> 00:02:47,660
+fibrinogen الى ال fibrin واللي هي ال clot و هال ال
+
+35
+00:02:47,660 --> 00:02:51,500
+clot ممكن تتجمع في أماكن مختلفة في الجسم و تم ال
+
+36
+00:02:51,500 --> 00:02:55,200
+plugging اغلاق لبعض ال capillaries او ال veins حتى
+
+37
+00:02:55,200 --> 00:02:59,820
+وبالتالي تقبل blood supply على areas على مناطق
+
+38
+00:02:59,820 --> 00:03:06,380
+كاملةLoss of blood supply اليوكوسايدين
+
+39
+00:03:06,380 --> 00:03:10,460
+والهيموليسين متخصصات في قتل نوع معين من الخلايا
+
+40
+00:03:10,460 --> 00:03:14,200
+النوع الأول اللي هو اليوكوسايدين ويوكوسايدس
+
+41
+00:03:14,200 --> 00:03:17,140
+والتانية اللي هي الارتراوسايدس كلمة هيموليسين تما
+
+42
+00:03:17,140 --> 00:03:23,220
+الهيموليسز لل red blood cellsواخرش هدفنا عن نوعين
+
+43
+00:03:23,220 --> 00:03:28,600
+من التوكسين حد اسمها anthrax elongation factor افن
+
+44
+00:03:28,600 --> 00:03:32,960
+edema factor والتاني pertosis adenylate cyclase
+
+45
+00:03:32,960 --> 00:03:36,320
+وكانوا التانين بيشتغلوا بنفس القدرة التانين
+
+46
+00:03:36,320 --> 00:03:40,540
+بيعملوا كتالة يا بنات بيعملوا activation لأنزيم
+
+47
+00:03:40,540 --> 00:03:46,060
+اسمه adenylate cyclase enzyme هذا الانزيم بيحول ال
+
+48
+00:03:46,060 --> 00:03:52,370
+ATP إلى cyclic AMPالـ Cyclic AMP بيعمل عملية
+
+49
+00:03:52,370 --> 00:03:56,130
+increase لل production of electrolytes .. مهم
+
+50
+00:03:56,130 --> 00:04:00,530
+بيعمل ال production هذا extra cellularly .. إيش
+
+51
+00:04:00,530 --> 00:04:04,030
+يعني extra cellularly؟ يعني خارج الخلية .. لأن لما
+
+52
+00:04:04,030 --> 00:04:08,210
+يصير فيه تركيز عالية من ال electrolytes خارج
+
+53
+00:04:08,210 --> 00:04:13,790
+الخلية، الدسم يميل إلى تخفيف .. إلى تخفيف هذه ال
+
+54
+00:04:13,790 --> 00:04:16,790
+electrolytes عبر إنتاج كمية كبيرة من مين؟ من
+
+55
+00:04:16,790 --> 00:04:22,360
+الساقلإذا كان ال electrolyte موجودة في تجويف ال
+
+56
+00:04:22,360 --> 00:04:26,760
+human زي مثلا ال intestine، بينتج عندي ديارية لأنه
+
+57
+00:04:26,760 --> 00:04:30,980
+سائل كمية كبيرة، إذا كان مالوش تجويف، بيصير عندي
+
+58
+00:04:30,980 --> 00:04:34,140
+إدمة أو الاستثقاء، اللي هي الانتفاخ الناتجة عن
+
+59
+00:04:34,140 --> 00:04:40,420
+وجود كمية كبيرة من مين؟ من الانتفاخ الثالث
+
+60
+00:04:40,420 --> 00:04:44,240
+من ال violence factors، اللي هو من التكسيفأحنا
+
+61
+00:04:44,240 --> 00:04:48,740
+قررنا نعامل صنفين based on the mechanism of
+
+62
+00:04:48,740 --> 00:04:52,260
+production endotoxins are integral part of the
+
+63
+00:04:52,260 --> 00:04:56,700
+cell component يعني الجزء الأساسي و لا تتحلل ولا
+
+64
+00:04:56,700 --> 00:05:01,300
+تطلق إلا بعد موت الخلية بينما ال exotoxins are
+
+65
+00:05:01,300 --> 00:05:04,900
+produced while the cells are alive عندما تكون
+
+66
+00:05:04,900 --> 00:05:12,380
+الخلية حية فحية و بتنتجطيب تخيلوا الأن أنها أنتجت
+
+67
+00:05:12,380 --> 00:05:18,280
+التوكسين تبعها في غذائنا غير علّما تنتجه داخل
+
+68
+00:05:18,280 --> 00:05:23,420
+أجسامنا و بعض التوكسين قد تنتج في داخل أجسامنا لكن
+
+69
+00:05:23,420 --> 00:05:27,240
+اتضال التأثير تبعها محصول في مكان محدد طبعا
+
+70
+00:05:27,240 --> 00:05:31,640
+المصطلحات اللي على السطورة produced in vitro و in
+
+71
+00:05:31,640 --> 00:05:33,820
+vivo بعرف المصطلحات هذي كم ولا انكم ولا لأ
+
+72
+00:05:33,820 --> 00:05:41,970
+انترجمهم in vivo يعنيإيش يعني؟ in test tube يعني
+
+73
+00:05:41,970 --> 00:05:49,190
+ليست في كائن حي معمليًا in vivo داخل الكائن الحي
+
+74
+00:05:49,190 --> 00:05:52,750
+فلما بنعمل experiment احنا بنسميها experiment إما
+
+75
+00:05:52,750 --> 00:05:55,970
+بنقول in vivo أو in vitro حسب وين إحنا إجرينا ال
+
+76
+00:05:55,970 --> 00:05:59,310
+experiment إذا عملناها في ال test tube عملناها في
+
+77
+00:05:59,310 --> 00:06:03,490
+ال petri dish دماغ بنقول عنها in vitro إذا عملناها
+
+78
+00:06:03,490 --> 00:06:09,010
+في الإنسان أو الحيوان بنسميها in vivoفإذا الـ
+
+79
+00:06:09,010 --> 00:06:12,730
+toxin produced in vivo ممكن يسبب food poisoning و
+
+80
+00:06:12,730 --> 00:06:15,230
+من أشهر الأمثلة ال botulinum toxin أو ال botulism
+
+81
+00:06:15,230 --> 00:06:19,690
+toxin و الاستفيلكوكال enterotoxin كلمة enterotoxin
+
+82
+00:06:19,690 --> 00:06:24,830
+الآن صلحنا مصطلح جديد كنا مصنفين exo و endو الآن
+
+83
+00:06:24,830 --> 00:06:28,210
+بكلمة عن entero و ال entero عبارة عن تصنيف لل
+
+84
+00:06:28,210 --> 00:06:32,530
+location where the toxin acts when it is some
+
+85
+00:06:35,740 --> 00:06:40,280
+بيشتر ال central nervous system بنسميه neurotoxin،
+
+86
+00:06:40,280 --> 00:06:46,400
+حسب مكان العمل، حسب مكان العمل، طب إذا بقتل كل
+
+87
+00:06:46,400 --> 00:06:50,180
+الخلايا، بنسميه cytotoxin
+
+88
+00:06:53,540 --> 00:06:58,360
+أو مصطلحات nephrotoxic يعني سام لمن؟ لخلايا الكلية
+
+89
+00:06:58,360 --> 00:07:05,000
+autotoxic سام للأذن يعني للعصب السمعي مثلا في
+
+90
+00:07:05,000 --> 00:07:09,920
+الأذن يكون متخصص لكن في النهاية هو تصنيف بحسب ال
+
+91
+00:07:09,920 --> 00:07:14,140
+site affected فابتفقنا
+
+92
+00:07:14,140 --> 00:07:16,780
+على ال enterotoxin و ال botulism و أنا بتقولكوا ال
+
+93
+00:07:16,780 --> 00:07:21,980
+botulism هو أقوى سم معروف للإنسان ينتج من قبل
+
+94
+00:07:21,980 --> 00:07:28,480
+كإنحيطو غالبا بتسمم الناس عبر تناول معلبات
+
+95
+00:07:28,480 --> 00:07:35,980
+المعلبات اللى بتكون مصنعة و لم نصل فاكرين لما
+
+96
+00:07:35,980 --> 00:07:39,780
+اتكلمت عن ال sterilization و قلنالكوا ان في مصطلح
+
+97
+00:07:39,780 --> 00:07:43,920
+اسمه commercial sterilization تعقيم تجارى لأن
+
+98
+00:07:43,920 --> 00:07:48,220
+التعقيم العادي ممكن عقب لكن ماتعطيش .. بظبط
+
+99
+00:07:48,220 --> 00:07:50,060
+فبالتالي
+
+100
+00:07:51,370 --> 00:07:57,950
+بصطرح تعقيم تجاري اشترت خلو الطعام من ال spores
+
+101
+00:07:57,950 --> 00:08:02,370
+الممرضة اللي من ضمنها مين؟ البوتلوني طيب produced
+
+102
+00:08:02,370 --> 00:08:07,130
+in vivo يعني داخل بسمنا احنا اتناولنا البكتيريا و
+
+103
+00:08:07,130 --> 00:08:10,010
+البكتيريا نمت جوا و بدأت تنجل ال toxin تبعها
+
+104
+00:08:10,010 --> 00:08:15,310
+داخلنا زي مين؟ زي ال deuterium من أحد أشهر وأخطر
+
+105
+00:08:15,310 --> 00:08:21,220
+الأمراض قبل 30 سنةوانزلوا كان الدفتيريا واحد من
+
+106
+00:08:21,220 --> 00:08:25,340
+الأمراض الخطيرة جدا أو أربعين سنة حتى اللي هو
+
+107
+00:08:25,340 --> 00:08:27,820
+الخناء بيسموه باللغة العربية تمام؟ أو العرب
+
+108
+00:08:27,820 --> 00:08:30,100
+بيسموها دفتيريا اللي هو الدفتيريا
+
+109
+00:08:33,130 --> 00:08:36,090
+اللي قلتلكوا من كتر ما ال .. ال .. ال ..
+
+110
+00:08:36,090 --> 00:08:40,970
+الانقباضات بتكون عنيفة قد ينكسر العمود الفقري
+
+111
+00:08:40,970 --> 00:08:44,710
+للمريض، تخيل العمود الفقري من .. عشان نكسره بدنا
+
+112
+00:08:44,710 --> 00:08:50,250
+قوة هائلة، لكن من شدة انقباض العضلات في المريض
+
+113
+00:08:50,250 --> 00:08:54,890
+اللي عندنا نفسه قد ينكسر العمود الفقريكانها الـ
+
+114
+00:08:54,890 --> 00:08:58,690
+streptococcal erythrogenic toxin، كلها بالثلاث
+
+115
+00:08:58,690 --> 00:09:01,730
+أنواع من التوكسين اللي ذكرناها دول الأمثلة، تنتج
+
+116
+00:09:01,730 --> 00:09:05,470
+in vitro، أفضل in vivo، والتأثير بتاعها بيكون
+
+117
+00:09:05,470 --> 00:09:09,290
+systemic، إيه يعني systemic؟ يعني بكل الجسم، بكل
+
+118
+00:09:09,290 --> 00:09:14,010
+الجسم، بيرم هناك بعض الميكروبات والتوكسين بيكون
+
+119
+00:09:14,010 --> 00:09:20,980
+لها local toxic effect، لأالتوكسين بنا systemic،
+
+120
+00:09:20,980 --> 00:09:23,880
+البكتيرى نفسها local، البكتيرى نفسها localized، بس
+
+121
+00:09:23,880 --> 00:09:28,560
+في الان localized ال .. البكتيريا والتوكسين زي
+
+122
+00:09:28,560 --> 00:09:31,880
+الكوليرا، الكوليرا في الأمعة مابتعزلهاش من الدم
+
+123
+00:09:31,880 --> 00:09:34,380
+ولا بتعزلها من أي مكان تاني، فقط من وين؟ من
+
+124
+00:09:34,380 --> 00:09:37,400
+الأمعة، فالتوكسين تبعها أيضا بكون تأثيره على
+
+125
+00:09:37,400 --> 00:09:42,390
+الأمعةنبنع اشريشيا كولاي هي non pathogenic في ال
+
+126
+00:09:42,390 --> 00:09:44,830
+intestinal tract لكن في منها exceptions أربع
+
+127
+00:09:44,830 --> 00:09:47,110
+أصنات، همد كلهم لاحقا، همد كلها بعد medical
+
+128
+00:09:47,110 --> 00:09:51,590
+microbiology واحدة منهم اسمها Enterotoxinic
+
+129
+00:09:51,590 --> 00:09:56,110
+Escherichia Coli، إذا الآن قدامنا على اللوحة تلت
+
+130
+00:09:56,110 --> 00:10:00,370
+نماذج، يا ريت نطلع عليهم كويس إذا ال toxin was
+
+131
+00:10:00,370 --> 00:10:04,070
+produced in vitro، إذا ال toxin was produced in
+
+132
+00:10:04,070 --> 00:10:09,910
+vivo، في مثلين، systemic و في أنها localالان انا
+
+133
+00:10:09,910 --> 00:10:14,530
+كنت في تصنيف ال toxins حد بيسموها A B Model ال A B
+
+134
+00:10:14,530 --> 00:10:18,690
+Model عبارة عن نموذج لتوكسين أو مجموعة توكسينات
+
+135
+00:10:18,690 --> 00:10:23,530
+خاصة مش خاصة هي ال Exotoxins مكوّنة من شقين بدا
+
+136
+00:10:23,530 --> 00:10:28,570
+الشق الأول بنسميه A واحفظه A Active والجزء التاني
+
+137
+00:10:28,570 --> 00:10:31,290
+B اللي هو زي ما موجود على الصدبرة أيضا
+
+138
+00:10:34,430 --> 00:10:37,650
+واطلع على ال animation اللى موجودة على يمين الشاشة
+
+139
+00:10:37,650 --> 00:10:42,250
+هلاجى إنه في خلية فيها receptor لمين ال receptor ل
+
+140
+00:10:42,250 --> 00:10:48,170
+B لكن عمل internalization لمن ل A و B هتكلم عنها
+
+141
+00:10:48,170 --> 00:10:54,670
+الآن هي B وهي A وهي ال receptor الارتباط لمن هيكون
+
+142
+00:10:54,670 --> 00:11:00,730
+الآن ل Bلكن ال internalization بتم لكل التوكسين
+
+143
+00:11:00,730 --> 00:11:05,330
+لكلهما بيصير release مين الأن اللي بيشتغل جوا؟ B
+
+144
+00:11:05,330 --> 00:11:10,330
+ولا A؟ B has no independent action مالوش أي عمل
+
+145
+00:11:10,330 --> 00:11:14,830
+مستقل فقط عملية إيصال التوكسين إلى المكان الموجود
+
+146
+00:11:14,830 --> 00:11:18,890
+طبعا العملية برضه كمان مهمة يعني لو شنا ال B من ال
+
+147
+00:11:18,890 --> 00:11:20,390
+A مش هيشتغل
+
+148
+00:11:23,360 --> 00:11:27,640
+مواضحة النقطة دي انه في عندي في ال toxin A B Model
+
+149
+00:11:27,640 --> 00:11:33,540
+A هو ال active و ال B بس بدنا نفهمش هانا ال
+
+150
+00:11:33,540 --> 00:11:40,540
+A نفترض انها في المنتصف و ال B ممكن تكون فجيها
+
+151
+00:11:40,540 --> 00:11:43,540
+تحتها على يمين على يسار ماحنا بحسب ما ننظر إليها
+
+152
+00:11:43,540 --> 00:11:48,960
+طيب لو كانت ال A في المنتصف و في عندي B و B و B و
+
+153
+00:11:48,960 --> 00:11:50,460
+B يعني أكتر من B
+
+154
+00:11:56,020 --> 00:12:01,260
+النسخ بيصير فرصة .. فرصة ان ال toxin يرتبط أسرع و
+
+155
+00:12:01,260 --> 00:12:05,120
+أسهل تمام؟ بالضبط .. بالضبط زي لو احنا بنجيب كورة
+
+156
+00:12:05,120 --> 00:12:09,380
+و ن .. و ن .. و نلحن فيها مسامير و نرميها على أي
+
+157
+00:12:09,380 --> 00:12:13,160
+جهة رميناها هلاجي مسامير وسط الأرض و مسامير
+
+158
+00:12:13,160 --> 00:12:16,600
+اقضالها في .. في اتجاهات متنوعة و مختلفة وبالتالي
+
+159
+00:12:16,600 --> 00:12:21,510
+فرصتها في الارتباط بتكون ايه؟ أعلىطيب برضه نسميه a
+
+160
+00:12:21,510 --> 00:12:25,890
+b model صحيح a one و five b's تمام لكن في الآخر هو
+
+161
+00:12:25,890 --> 00:12:31,030
+a و b طب لو كان a اتنين و خمسة b بيمشي الحال بيمشي
+
+162
+00:12:31,030 --> 00:12:35,810
+الحال بيبقى نفس المفهوم two a's تمام و يمكن a one
+
+163
+00:12:35,810 --> 00:12:39,770
+و a two اخدوا بالكوا a one و a two ايش معناهم ان a
+
+164
+00:12:39,770 --> 00:12:46,420
+بختلف عن a one بختلف عن a twoA1, A2, 5B's، الـ
+
+165
+00:12:46,420 --> 00:12:50,240
+5B's هم هما و ممكن زي ما حضرتك اتوقعتي انه ناجي A
+
+166
+00:12:50,240 --> 00:12:54,960
+واحدة و 5B's مختلفين و هذا بيصير .. ايش بيعمل هذا؟
+
+167
+00:12:54,960 --> 00:13:00,560
+بيزيد من ال specificity تبعت للأنشجة المختلفة بصير
+
+168
+00:13:00,560 --> 00:13:04,500
+قدرة التوكسينة على الدخول الى أنشدة متنوعة اعلى
+
+169
+00:13:04,500 --> 00:13:07,620
+لما يكون في عنده اتنوة في ال B أو في ال binding
+
+170
+00:13:07,620 --> 00:13:13,920
+siteمن النماذج المشهورة جدا في علم ال microbiology
+
+171
+00:13:13,920 --> 00:13:17,140
+على ال mechanism of action ل A-B model اللي هو
+
+172
+00:13:17,140 --> 00:13:22,060
+deuterium toxin و طبعا في شبيه له اسمه pseudomonas
+
+173
+00:13:22,060 --> 00:13:26,420
+aeruginosa toxin A او X-toxin A كيف بيشتغلها
+
+174
+00:13:26,420 --> 00:13:30,160
+دولها؟ تقريبا بيشتغلوا بنفس القالية بس خدنا ناخد
+
+175
+00:13:30,160 --> 00:13:33,720
+ال deuterium كنموذج و التاني بنقول مثله بالضبط
+
+176
+00:13:33,720 --> 00:13:40,240
+تعرف ال protein synthesis؟ ال protein synthesisالـ
+
+177
+00:13:40,240 --> 00:13:43,400
+protein synthesis بيتلخص بإنه في عندي large
+
+178
+00:13:43,400 --> 00:13:47,720
+subunit و small subunit و messenger mRNA و بيصير
+
+179
+00:13:47,720 --> 00:13:52,500
+ارتباط بين التلتة component دولة و ببدأ ال .. ال
+
+180
+00:13:52,500 --> 00:13:55,700
+.. ال akyl aminotransfer يجي ينزل على ال ribosome
+
+181
+00:13:55,700 --> 00:13:58,640
+و يفرق حمولته من ال amino acid و ال amino acid
+
+182
+00:13:58,640 --> 00:14:03,180
+يرتبط ينتقل لقدر amino akyl و هكذا و يصير في إطالة
+
+183
+00:14:03,180 --> 00:14:09,380
+للسلسلةكلمة elongation factor بتعني عامل يساهم في
+
+184
+00:14:09,380 --> 00:14:15,980
+إطالة ال polypeptide اللي هو ال EF وهذا العامل من
+
+185
+00:14:15,980 --> 00:14:19,740
+ضرورات ال protein synthesis من ضرورات ال protein
+
+186
+00:14:19,740 --> 00:14:26,080
+synthesis في عملية اللي هو ال translation طيب
+
+187
+00:14:26,080 --> 00:14:29,240
+تخيلوا معا الوضع كان تاني ال diphtheria toxin
+
+188
+00:14:29,240 --> 00:14:34,880
+بتروح ترتبط بهذا ال factorو بتضيف إليه ADP-ripose
+
+189
+00:14:34,880 --> 00:14:40,480
+وعلى اللي بنسميها ADP-riposylation ADP اللي هو
+
+190
+00:14:40,480 --> 00:14:49,840
+adenosine diphosphate الآن ضفنا ADP-ripose لمين؟
+
+191
+00:14:49,840 --> 00:14:55,640
+لل elongation factor 2 اللي كان active لما ضفناله
+
+192
+00:14:55,640 --> 00:15:00,120
+بطل active لما بطل active يبقى الآن بطل يكون في
+
+193
+00:15:00,120 --> 00:15:03,910
+دورة تصنيعة لبروتينطيب بالنسبة لي احنا بتتكلم
+
+194
+00:15:03,910 --> 00:15:07,610
+دايما هو باستمرار خلية بدون protein synthesis is a
+
+195
+00:15:07,610 --> 00:15:12,210
+doomed dead يعني محكم عليها بالموت لماذا محكم
+
+196
+00:15:12,210 --> 00:15:15,910
+عليها بالموت؟ كل ال enzymes اللي بتشتغل في الخلية
+
+197
+00:15:15,910 --> 00:15:19,990
+هي proteins وبالتالي بدون تصنيع enzymes أو other
+
+198
+00:15:19,990 --> 00:15:24,770
+component الخلية بالموت وبالتالي اي toxin
+
+199
+00:15:27,350 --> 00:15:33,450
+بيواجه تصنيع ال protein irreversibly بشكل غير قابل
+
+200
+00:15:33,450 --> 00:15:37,530
+للعكس، غير قابل للعكس، غالبا .. غالبا بيؤدي لموت
+
+201
+00:15:37,530 --> 00:15:38,710
+اللي هو ايه؟ الخلق
+
+202
+00:15:58,120 --> 00:16:00,920
+طب طبطبطبطبطبطبطبطبطبطبطبطبطبطبطبطبطبطبط
+
+203
+00:16:15,080 --> 00:16:19,360
+بترتبط بال adenylate cyclase enzyme، ارتباطها بال
+
+204
+00:16:19,360 --> 00:16:22,000
+adenylate cyclase enzyme مش بيعمل، بيعمله
+
+205
+00:16:22,000 --> 00:16:26,360
+activation لها، عكس الأولانية اللي كانت عمل بيه
+
+206
+00:16:26,360 --> 00:16:30,400
+activation، إذا بالارتباط، خدوا بالكمون التوكسين
+
+207
+00:16:30,400 --> 00:16:32,780
+في المركب، يا بعمله activation يا بعمله
+
+208
+00:16:32,780 --> 00:16:37,120
+inactivation، فهذا عملله activation، لما عملله
+
+209
+00:16:37,120 --> 00:16:42,480
+activation، راح حوّل ال ATP لcyclic AMPوالcycle
+
+210
+00:16:42,480 --> 00:16:46,980
+kmb انشرحته المرة الماضية قلنا بيزيد من اللي هيطلع
+
+211
+00:16:46,980 --> 00:16:52,080
+على الصبورة على اللوحة أيضا في ان هنا كمية الانتاج
+
+212
+00:16:52,080 --> 00:16:56,480
+لل Sodium و لل chloride و لل potassium و لل
+
+213
+00:16:56,480 --> 00:17:01,220
+carbonate زادت في ال human of the intestine فالجسم
+
+214
+00:17:01,220 --> 00:17:05,800
+مضطر ينزل معاشر كمان كمية كبيرة من من الماء وبتالي
+
+215
+00:17:05,800 --> 00:17:09,550
+دياريا واز producedإذا هذه الـ mechanism by which
+
+216
+00:17:09,550 --> 00:17:16,030
+ال Vibrio cholera produces دياري اكيوميليش .. نجيب
+
+217
+00:17:16,030 --> 00:17:18,550
+معكوس، نقول دياريا was produced، ليش فيه ديارية؟
+
+218
+00:17:18,550 --> 00:17:21,550
+لأنه فيه ميه، ليش فيه ميه كبيرة، كمية كبيرة؟ لأنه
+
+219
+00:17:21,550 --> 00:17:24,810
+فيه electrolytes طب ليش ال electrolytes اتراكمت في
+
+220
+00:17:24,810 --> 00:17:29,130
+المكان هذا؟ لأنه فيه كمية Cyclic AMP أُنتجات بشكل
+
+221
+00:17:29,130 --> 00:17:32,730
+كبير، لماذا؟ لأنه فيه cell activation لليليت
+
+222
+00:17:32,730 --> 00:17:35,530
+الcyclase enzyme، ميه اللي عمل activation؟ اللي هو
+
+223
+00:17:35,530 --> 00:17:41,150
+ال cholera toxicوهناك Escherichia coli بتنتج
+
+224
+00:17:41,150 --> 00:17:45,250
+توكسين اسمه cholera-like toxin اللي هي البكتيريا
+
+225
+00:17:45,250 --> 00:17:49,090
+اللي اسمها enterotoxygenic escherichia coli
+
+226
+00:17:49,090 --> 00:17:54,090
+enterotoxygenic escherichia coli و بين قوسين حطوا
+
+227
+00:17:54,090 --> 00:17:57,730
+كلمة labile ماعرفش مولود على اللابحة كلمة labile
+
+228
+00:17:57,730 --> 00:18:04,970
+شايفينها? E coli labile toxinكلمة labile يعني قابل
+
+229
+00:18:04,970 --> 00:18:10,230
+للتدمير .. قابل للتدمير لما عزلوا ال E.coli toxins
+
+230
+00:18:10,230 --> 00:18:14,370
+لجوا منها نوعين لجوا نوع stable التكسين تبعها لما
+
+231
+00:18:14,370 --> 00:18:19,110
+نحطه بالحرارة اللي مدملش فسموه S أو stable toxin
+
+232
+00:18:19,110 --> 00:18:24,070
+والتاني بتأثر بالحرارة فسموه labile toxin إذا ال
+
+233
+00:18:24,070 --> 00:18:25,930
+labile toxin هو اللي بتتكلم عنه
+
+234
+00:18:32,130 --> 00:18:36,110
+يعني هيمروا معاها في المستقبل oxygen labile هداك
+
+235
+00:18:36,110 --> 00:18:39,610
+هنقوله عنها heat labile تتأثر بالحرارة أو تتأثر
+
+236
+00:18:39,610 --> 00:18:48,590
+بمين؟ بال oxygen طيب نجي لتكسين تنتجه الشجلة أو
+
+237
+00:18:48,590 --> 00:18:50,950
+برضه نوع آخر من الشريشا كولا يسمونه
+
+238
+00:18:50,950 --> 00:18:56,270
+enterohemorrhagic-schericia-kola إذن نتكلم عن two
+
+239
+00:18:56,270 --> 00:19:03,990
+toxins بينتلكوا نفس ال mechanism ofبرضه في المحصلة
+
+240
+00:19:03,990 --> 00:19:08,190
+بيوقفوا ال protein synthesis هنا لكن بطريقة مختلفة
+
+241
+00:19:08,190 --> 00:19:11,990
+عن الدفتيريا تتذكروا الدفتيريا اشتغلت على مين؟ على
+
+242
+00:19:11,990 --> 00:19:16,710
+elongation factor 2 هذه اشتغلت على مين؟ على انها
+
+243
+00:19:16,710 --> 00:19:20,570
+عملت تكسير للريبوزومة ال RNA اللي في الريبوزوم
+
+244
+00:19:20,570 --> 00:19:22,990
+المهمين اللي بيصنع ال protein synthesis أساسا
+
+245
+00:19:22,990 --> 00:19:26,630
+الريبوزوم إذا خربنا الريبوزوم و بقوا فينا ال
+
+246
+00:19:26,630 --> 00:19:29,690
+protein synthesis يبقى وجفنا و أصنع فرده ال
+
+247
+00:19:29,690 --> 00:19:37,430
+protein لكن بطريقةمختلفة عن اللي هو ال .. نعم نجد
+
+248
+00:19:37,430 --> 00:19:42,190
+البوتلونيو توكسين البوتلونيو توكسين محتاج مننا
+
+249
+00:19:42,190 --> 00:19:46,600
+يمكن ان شاء الله نقدر نوركوا ال animationبنطلع على
+
+250
+00:19:46,600 --> 00:19:49,940
+ال animation اللي قدامنا هذه ونشوف ال X-Toxins
+
+251
+00:19:49,940 --> 00:19:53,700
+طبعا قلنا they are produced while the cells are
+
+252
+00:19:53,700 --> 00:19:58,180
+alive and their effect may be localized or may be
+
+253
+00:19:58,180 --> 00:20:01,780
+systemic على حسب اللي هو النوع التوكسين زي ال
+
+254
+00:20:01,780 --> 00:20:03,980
+Vibrio كده اللي قلنا localized بينما التتانوس كان
+
+255
+00:20:03,980 --> 00:20:09,580
+إيه؟ كان سيستميك تصنيف ال X-Toxin قلنا ممكن يكون
+
+256
+00:20:09,580 --> 00:20:15,340
+حسب اللي هو ال site affectedيعني مثلا لما قلنا انه
+
+257
+00:20:15,340 --> 00:20:21,060
+بصيب ال nervous system سمنة neurotoxin إذا بصيب ال
+
+258
+00:20:21,060 --> 00:20:24,700
+intestine سمنة ال intestine ال intestine ال
+
+259
+00:20:24,700 --> 00:20:25,140
+intestine ال intestine ال intestine ال intestine
+
+260
+00:20:25,140 --> 00:20:25,140
+ال intestine ال intestine ال intestine ال
+
+261
+00:20:25,140 --> 00:20:25,380
+intestine ال intestine ال intestine ال intestine
+
+262
+00:20:25,380 --> 00:20:25,540
+ال intestine ال intestine ال intestine ال
+
+263
+00:20:25,540 --> 00:20:26,000
+intestine ال intestine ال intestine ال intestine
+
+264
+00:20:26,000 --> 00:20:26,000
+ال intestine ال intestine ال intestine ال
+
+265
+00:20:26,000 --> 00:20:26,980
+intestine ال intestine ال intestine ال intestine
+
+266
+00:20:26,980 --> 00:20:27,020
+ال intestine ال intestine ال intestine ال
+
+267
+00:20:27,020 --> 00:20:27,840
+intestine ال intestine ال intestine ال intestine
+
+268
+00:20:27,840 --> 00:20:27,840
+ال intestine ال intestine ال intestine ال
+
+269
+00:20:27,840 --> 00:20:27,860
+intestine ال intestine ال intestine ال intestine
+
+270
+00:20:27,860 --> 00:20:27,860
+ال intestine ال intestine ال intestine ال
+
+271
+00:20:27,860 --> 00:20:27,900
+intestine ال intestine ال intestine ال intestine
+
+272
+00:20:27,900 --> 00:20:27,900
+ال intestine ال intestine ال intestine ال
+
+273
+00:20:27,900 --> 00:20:27,940
+intestine ال intestine ال intestine ال intestine
+
+274
+00:20:27,940 --> 00:20:27,940
+ال intestine ال intestine ال intestine ال
+
+275
+00:20:27,940 --> 00:20:27,940
+intestine ال intestine ال intestine ال intestine
+
+276
+00:20:27,940 --> 00:20:27,940
+ال intestine ال intestine ال intestine ال
+
+277
+00:20:27,940 --> 00:20:27,940
+intestine ال intestine ال intestine ال intestine
+
+278
+00:20:27,940 --> 00:20:27,940
+ال intestine ال intestine ال intestine ال
+
+279
+00:20:27,940 --> 00:20:27,940
+intestine ال intestine ال intestine ال intestine
+
+280
+00:20:27,940 --> 00:20:28,040
+ال intestine ال intestine ال intestine ال
+
+281
+00:20:28,040 --> 00:20:28,040
+intestine ال intestine ال intestine ال intestine
+
+282
+00:20:28,040 --> 00:20:28,320
+ال intestine ال intestine ال intestine ال
+
+283
+00:20:28,320 --> 00:20:28,320
+intestine ال intestine ال intestine ال intestine
+
+284
+00:20:28,320 --> 00:20:32,660
+ال intestine ال intestine ال intestine ال
+
+285
+00:20:32,660 --> 00:20:36,340
+intestine ال
+
+286
+00:20:44,400 --> 00:20:47,540
+التصنيف الان by structure and function هذا تصنيفه
+
+287
+00:20:47,540 --> 00:20:51,720
+كويس كثير احنا قلنا اب موديل صح الاب موديل اذا كان
+
+288
+00:20:51,720 --> 00:20:55,620
+تركيبه من قطعتين اساسيتين active و binding بيسمي
+
+289
+00:20:55,620 --> 00:20:59,280
+اب توكسين وفي membrane disruption اللي هو بيأثر
+
+290
+00:20:59,280 --> 00:21:02,820
+على ال .. ال .. ال .. ال membrane خدنا ناخد الأول
+
+291
+00:21:02,820 --> 00:21:06,540
+حاجة اللي هو الاب توكسين وطبعا الأخر اللي هو كان
+
+292
+00:21:06,540 --> 00:21:10,720
+super antigen هاي الالومان والجزء التاني هو ال بي
+
+293
+00:21:10,720 --> 00:21:13,260
+لومان ال بي كان لل binding
+
+294
+00:21:16,520 --> 00:21:20,240
+احنا معناه نفهم ال concepts بشكل أساسي عشان نقدر
+
+295
+00:21:20,240 --> 00:21:24,080
+نفهم بعض النقاط المتلتبة على هذا ال toxin لاحقا
+
+296
+00:21:24,080 --> 00:21:27,860
+بال endosomes بعدين بيصيرنه release و اللي هو ال a
+
+297
+00:21:27,860 --> 00:21:29,940
+هو اللي بيقوم بالمهمة تبعه
+
+298
+00:21:37,250 --> 00:21:41,330
+طبعا ال effects مختلفة ال effects مختلفة بحسب
+
+299
+00:21:41,330 --> 00:21:44,530
+التوكسين لكن احنا بنوصل الفكرة ان ال B هو اللي دخل
+
+300
+00:21:44,530 --> 00:21:48,190
+A و بعدين صارله release ال membrane disruption
+
+301
+00:21:48,190 --> 00:21:51,910
+فكان ال phospholipase و ال lycethinase و ال
+
+302
+00:21:51,910 --> 00:21:55,210
+leukocydenes كل مدلة بيشتغلوا على مين؟ على ال
+
+303
+00:21:55,210 --> 00:21:59,030
+membranes فلو بيعملوا complex معين بيفتح ال
+
+304
+00:21:59,030 --> 00:22:02,710
+membrane بصير release لمين؟ لل component سبعة
+
+305
+00:22:02,710 --> 00:22:04,930
+الخلية هذا بصير موط ليه؟ الخلية
+
+306
+00:22:08,300 --> 00:22:11,480
+النوع التالت هو ال super antigen وانا بدأ اشرح هو
+
+307
+00:22:11,480 --> 00:22:13,880
+يعني مش كتير واضح اللي .. اللي .. اللي موجود في ال
+
+308
+00:22:13,880 --> 00:22:18,120
+animation بس برضه كويس اننا نطلع عليه نجح فهنا
+
+309
+00:22:18,120 --> 00:22:24,640
+الان لما انا .. لو انا في المحاضرة بدوزع حدث معين
+
+310
+00:22:24,640 --> 00:22:31,060
+على الطلاب فبقول لزملتكوا انت عليكي واجب X وانت
+
+311
+00:22:31,060 --> 00:22:37,700
+واجب Y و Z و A و C بنصق الموضوعلكن لو افترضنا ..
+
+312
+00:22:37,700 --> 00:22:42,480
+لو افترضنا ان الشخص اللي بوزع المهم مش موجود و
+
+313
+00:22:42,480 --> 00:22:46,660
+ايجا واحد انت عليك مهمة X وانت مهمة X وانت مهمة X
+
+314
+00:22:46,660 --> 00:22:50,740
+وانت مهمة X بصير اللي بيعملوا نفس المهمة كثق
+
+315
+00:22:50,740 --> 00:22:59,990
+فالمنتج تبع المهمة برضه كتيراحنا بنعرف .. بنعرف ان
+
+316
+00:22:59,990 --> 00:23:04,130
+ال .. ال .. ال immune system is orchestrated ..
+
+317
+00:23:04,130 --> 00:23:06,070
+orchestrated يعني منظم زي orchestra بتاخد
+
+318
+00:23:06,070 --> 00:23:08,710
+orchestra اللي بيبقى مسك العصايا و ي .. و ينظم ال
+
+319
+00:23:08,710 --> 00:23:13,290
+.. ال .. اللي باعزفه موسيقى ال immune system
+
+320
+00:23:13,290 --> 00:23:16,410
+بيوصفوه بهذه الطريقة انه orchestrated انه في خلايا
+
+321
+00:23:16,410 --> 00:23:24,430
+بتنظم عمل الخلايا الأخرى إنتاج T cell لوظيفةإنها
+
+322
+00:23:24,430 --> 00:23:29,510
+تجيب تتفرز cytokines ال cytokines اتنادي فيها على
+
+323
+00:23:29,510 --> 00:23:33,890
+other cells cytokines specific تخيلوا كل الصف
+
+324
+00:23:33,890 --> 00:23:39,430
+سيطلع cytokines زي ما كل الصف بصير صوت حد هيفهم ..
+
+325
+00:23:39,430 --> 00:23:44,670
+هيفهم انه جهاز المناعة كله هيجي على المكان طيب
+
+326
+00:23:44,670 --> 00:23:49,870
+احنا ردة الفعل اللي المطلوبة هي ردة فعل تتنسب مع
+
+327
+00:23:49,870 --> 00:23:55,300
+حجم ال antigenلما الانتجين هذا يعمل رد فعل مناعية
+
+328
+00:23:55,300 --> 00:23:59,600
+ضخمة بيلاقيش بسرعانها بسرعانها exaggerated immune
+
+329
+00:23:59,600 --> 00:24:04,860
+response يعني رد فعل مناعية مبالغ بها تؤدي إلى
+
+330
+00:24:04,860 --> 00:24:08,540
+ماذا؟ إلى release لاهستامين و inflammatory
+
+331
+00:24:08,540 --> 00:24:17,080
+substances و قد تؤدي إلى shock and death تخيلوا؟
+
+332
+00:24:17,080 --> 00:24:21,720
+من ايه؟ من انه ليه ليه يسموه super antigen؟أكيد
+
+333
+00:24:21,720 --> 00:24:25,880
+يقولوا super يعني حاجة مميزة، means تو الأساسية
+
+334
+00:24:25,880 --> 00:24:31,240
+فقط أنه بيقدر يعمل non-specific activation of news
+
+335
+00:24:52,510 --> 00:24:56,870
+أما لما يكون localized الأمر متابعه بيكون ان شاء
+
+336
+00:24:56,870 --> 00:24:57,330
+الله بسيط
+
+337
+00:25:00,690 --> 00:25:06,470
+الخلايا يشتر فيها انقسامات كمية كبيرة produced من
+
+338
+00:25:06,470 --> 00:25:10,450
+ال cytokines لا تتنسب إطلاقا مع حجم ال threat
+
+339
+00:25:16,390 --> 00:25:20,310
+لحظوا انا لففكت على مين؟ على كل ال pc لأن كل جهاز
+
+340
+00:25:20,310 --> 00:25:23,670
+المناعة بيتأثر بهذا و طبعا بيصير فيه ارتفاع في
+
+341
+00:25:23,670 --> 00:25:27,190
+درجة حرارة اللي هو ال pc مدام صارنا داخلين في ال
+
+342
+00:25:27,190 --> 00:25:31,530
+animation نكمل و نشوف ال endotoxin و ذكركم احنا
+
+343
+00:25:31,530 --> 00:25:37,840
+نستعرض اللوحة، يا عزيزي نتركز على اللوحةإن هو
+
+344
+00:25:37,840 --> 00:25:40,480
+عبارة عن component من ال cell من ال outer cell من
+
+345
+00:25:40,480 --> 00:25:43,080
+بنيه ب gram negative بكتيريا وكان مكوّن من O-sided
+
+346
+00:25:43,080 --> 00:25:45,840
+chain و ال lipid A، ال lipid A هو ال endotoxin
+
+347
+00:25:45,840 --> 00:25:50,980
+الماكروفاج بتاكل البكتيريا أو ال phagocyte، جوا
+
+348
+00:25:50,980 --> 00:25:55,260
+الماكروفاج بيصير تم عملية اللي هو التحليل لل .. ال
+
+349
+00:25:55,260 --> 00:25:59,990
+.. ال ..البكتيريا وتكسيرها بيصير release لمن؟ لل
+
+350
+00:25:59,990 --> 00:26:04,910
+endotoxin بيطلع انرسحانس الو Interleukin ال
+
+351
+00:26:04,910 --> 00:26:08,310
+Interleukin بيروح على ال hypothالمس في الدماغ
+
+352
+00:26:08,310 --> 00:26:11,710
+والان ال hypothالمس مسئول عن ال thermal regulation
+
+353
+00:26:11,710 --> 00:26:17,670
+في الجسم فبترتفع درجة الحرارة أول حاجة بترتفع درجة
+
+354
+00:26:17,670 --> 00:26:22,570
+الحرارة طبعا في أشياء أخرى كتيرة من ضمنها العميدي
+
+355
+00:26:22,570 --> 00:26:24,770
+الفاجو سايتوز الكتيرة ال antibodies اللي بيصير
+
+356
+00:26:24,770 --> 00:26:30,520
+تتنشفلكن في نقطة كتير مهمة، وجود البكتيريا
+
+357
+00:26:30,520 --> 00:26:36,750
+والتوكسين بيقدي الى انخفاض في طاقة الدمأحد أهم
+
+358
+00:26:36,750 --> 00:26:40,950
+أسباب الخطورة في ال endotoxin هو انخفاض اللي هو ال
+
+359
+00:26:40,950 --> 00:26:42,450
+blood pressure اللي هو الأصل اللي .. الأصل اللي
+
+360
+00:26:42,450 --> 00:26:46,630
+لازم يكون على كده؟ 120 على 80 مثلا أو 110 على 70
+
+361
+00:26:46,630 --> 00:26:50,730
+بحسب الجنس ذاك على المنثى، لاحظوا أنا مش بصير أنا
+
+362
+00:26:50,730 --> 00:26:55,370
+جاعد، بسبب هبوط في ضغط الدم، طب لما يصل أنا ضغط
+
+363
+00:26:55,370 --> 00:27:00,830
+الدم لهذا .. لهذه الدرجة، أي حركة قد تفقدنا
+
+364
+00:27:00,830 --> 00:27:04,710
+التوازن أو ممكن حتى نصل بينا إلى shock and death
+
+365
+00:27:05,210 --> 00:27:08,850
+فال endotoxin يا بنات التوكسين تبع ال gram
+
+366
+00:27:08,850 --> 00:27:12,410
+negative بكتيريا اللي احنا اتعلمناه في أول الفصل و
+
+367
+00:27:12,410 --> 00:27:16,370
+قلنا عنه جزء من ال component تبع كل gram negative
+
+368
+00:27:16,370 --> 00:27:21,990
+بكتيريا لو البكتيريا موجودة فيها جسمنا و علجناها و
+
+369
+00:27:21,990 --> 00:27:28,450
+قضينا عليها هذا وحده لا يكفيلأنه قد نقتل المريض
+
+370
+00:27:28,450 --> 00:27:32,210
+بقتل الألمين للبكتيريا وبالتالي بدنا نكون عندنا
+
+371
+00:27:32,210 --> 00:27:36,390
+اطلاع او معرفة بأن ال endotoxin له effects كثيرة
+
+372
+00:27:36,390 --> 00:27:40,090
+من ضمنها كذا مرة low blood pressure high
+
+373
+00:27:40,090 --> 00:27:44,010
+temperature وفي عندنا اللي هو coma and death طب
+
+374
+00:27:44,010 --> 00:27:48,470
+خليني بس نوضح النقطة هذه الان انتوا بتعرفوا مركبة
+
+375
+00:27:48,470 --> 00:27:52,310
+ال acetylcholine اسمها فيه؟ ايش هو دي بتقولها ده؟
+
+376
+00:27:52,310 --> 00:27:58,520
+neurotransmitter ايش هي transmission؟Bath، bath
+
+377
+00:27:58,520 --> 00:28:02,560
+يعني نقل إشارة، فال neurotransmitter هو نقل عصبي،
+
+378
+00:28:02,560 --> 00:28:06,500
+نقل عصب أو إشارة عصبية، ال botulinum toxin حضرتوش
+
+379
+00:28:06,500 --> 00:28:10,760
+بيعمل .. بيعمل inhibition للأزيطائي الكلي، يبقى
+
+380
+00:28:10,760 --> 00:28:14,400
+مافيش نقل للإشارة العصبية، مافيش إرسال، مافيش
+
+381
+00:28:14,400 --> 00:28:17,540
+إرسال .. مافيش إرسال يبقى مافيش استجبال، وده لا ..
+
+382
+00:28:17,540 --> 00:28:21,080
+وده مافيش استجبال يبقى فينا شلل، أي نوع من الشلل
+
+383
+00:28:22,710 --> 00:28:25,530
+الacetylcholine المفروض أن يعطينا إشارة
+
+384
+00:28:25,530 --> 00:28:28,450
+للcontraction، ما تنفيش contraction، إيش معناه
+
+385
+00:28:28,450 --> 00:28:32,650
+حاجة تكون فينا؟ relaxation، شلل بيسموه أخر جملة
+
+386
+00:28:32,650 --> 00:28:36,010
+flaccid paralysis، فالـ flaccid paralysis معناته
+
+387
+00:28:36,010 --> 00:28:40,200
+شلل إرتخائي، شلل إرتخائيو انا فاكرين لما اتكلمنا
+
+388
+00:28:40,200 --> 00:28:45,020
+عن ال toxin هذا قلنالكم بيعطوه ك .. للتجميل في ال
+
+389
+00:28:45,020 --> 00:28:49,760
+botox او ما يسمى ال botox و قلنا سبب استخدامه هو
+
+390
+00:28:49,760 --> 00:28:53,100
+ان التجايد هي عبارة عن .. يعني بتكون تقلصات
+
+391
+00:28:53,100 --> 00:28:56,300
+contracted skin او .. او muscles في ال skin فهو
+
+392
+00:28:56,300 --> 00:28:59,080
+لما لها عملته اللي هو relaxation لكن هذا قلنا كلمة
+
+393
+00:28:59,080 --> 00:29:04,100
+مرة قوله diluted بيكون في فترة مؤقتةلكن بنفهم إن
+
+394
+00:29:04,100 --> 00:29:06,960
+الـ mechanism of action كانت مبنية على مين؟ على
+
+395
+00:29:06,960 --> 00:29:12,680
+إنه فقدان الإشارة العصبية عن طريق منع إنتاج من الـ
+
+396
+00:29:12,680 --> 00:29:17,060
+sparkling أنا بقولكوا احفظوا لإنه اللي وراه على
+
+397
+00:29:17,060 --> 00:29:25,700
+طول اللي هو التتانوس عكسه تمانع inhibits
+
+398
+00:29:25,700 --> 00:29:30,020
+glycine release إيش مشكلة ال glycine هذا؟ الآن
+
+399
+00:29:30,020 --> 00:29:34,960
+مافيش botulism فيه normalفي normal contraction و
+
+400
+00:29:34,960 --> 00:29:38,200
+relaxation ال contraction بينتج على أنه في
+
+401
+00:29:38,200 --> 00:29:43,340
+أسيتالكولين تمام؟ و ال relaxation لجليسين بيقول ل
+
+402
+00:29:43,340 --> 00:29:46,720
+.. بيقول لجليسين بيقول لأسيتالكولين بكف خلاص روح
+
+403
+00:29:46,720 --> 00:29:50,120
+تمام؟ فبصير العملية فيها نوع من إيه؟ من الإتزان
+
+404
+00:29:50,120 --> 00:29:54,400
+الأسيتالكولين بيعطي الإشارة و أنا بقطع النوم
+
+405
+00:29:54,400 --> 00:29:59,120
+الإشارة فبصير العملية طبيعية طيب في ال tetanus
+
+406
+00:29:59,120 --> 00:30:05,100
+الوضع اختلف في أسيتالكولينوعمل contraction احنا
+
+407
+00:30:05,100 --> 00:30:09,560
+مانعنا مين؟ مانعنا اللي بيمنع ال acetylcholine
+
+408
+00:30:09,560 --> 00:30:13,800
+فصار عايش عندي contraction على طول بيبقى الشرر
+
+409
+00:30:13,800 --> 00:30:16,400
+اللي صار عندي شرر مختلف تماما عن الأولاني هدا
+
+410
+00:30:16,400 --> 00:30:19,860
+الأولاني إيش كان؟ كان relaxed أو سبب لها flaccid
+
+411
+00:30:19,860 --> 00:30:24,460
+paralysis هذا بيسموه spastic، spastic يعني انقباضي
+
+412
+00:30:24,460 --> 00:30:29,580
+وعشان قلتلكوا أنه ال spasm أو ال .. ال .. ال ..
+
+413
+00:30:30,210 --> 00:30:34,730
+التقلصات أو التشهنجات اللى تنتج عند مرضى التتانوس
+
+414
+00:30:34,730 --> 00:30:40,110
+قد تكون شهيدة وتكسر العمود اللى هو Alpha ال
+
+415
+00:30:40,110 --> 00:30:42,850
+animation اللى قدامنا هذا على فكرة واضحة برضه
+
+416
+00:30:42,850 --> 00:30:48,850
+بتعطينا فكرة على اللى هو العملية بتاعة الدهام
+
+417
+00:30:48,850 --> 00:30:54,310
+الأولفي عندي هناش ال acetylcholine receptor و هذا
+
+418
+00:30:54,310 --> 00:30:58,810
+ال glycine اللي هو ال glycine لإن ال toxin بيرفبط
+
+419
+00:30:58,810 --> 00:31:02,210
+بي هذا المنطقة و بيمنع مين؟ بيمنع ال glycine
+
+420
+00:31:02,210 --> 00:31:06,430
+فبالتالي بيصير فيه إنتاج مستمر لل acetylcholine و
+
+421
+00:31:06,430 --> 00:31:09,490
+action مستمر لل acetylcholine و بالتالي مافيش أنها
+
+422
+00:31:09,490 --> 00:31:14,830
+ان كان هي لأ فإنها contraction مستمر و لا يوجد
+
+423
+00:31:14,830 --> 00:31:20,090
+relaxationوهذا ال .. ال .. مريض .. مريض تتنوس بي
+
+424
+00:31:20,090 --> 00:31:25,670
+.. يعني بيمر براحة كتيرة من ضمنها اللي هو ال
+
+425
+00:31:25,670 --> 00:31:29,330
+paralysis لعضلات الفك لذلك باللي جيه بالعربي
+
+426
+00:31:29,330 --> 00:31:33,670
+بيسموه الكزاز ليش؟ لإنه كز ع أصنانه لإن عضلات ..
+
+427
+00:31:33,670 --> 00:31:37,890
+أو بيسموه بالإنجليزي fixed smile الإبتسامة الثابت
+
+428
+00:31:37,890 --> 00:31:41,330
+دايما المريض بيكون مبتسم وعشانك مش كل واحد بيبتسم
+
+429
+00:31:41,330 --> 00:31:44,450
+إنه ممكن .. يعني يكون مبتسم إنه سعيد ربما يكون
+
+430
+00:31:44,450 --> 00:31:52,620
+عنده تتنوسطيب ال X-toxin في ال .. ال .. في ال
+
+431
+00:31:52,620 --> 00:31:56,000
+extra cellular matrix of connective tissue مثال
+
+432
+00:31:56,000 --> 00:32:01,540
+احنا درسنا في ال invasions تمام بعض التوكسينز
+
+433
+00:32:01,540 --> 00:32:05,140
+الكرستوديوم بقى رفرنجز على سبيل المثال بتنتج
+
+434
+00:32:05,140 --> 00:32:07,240
+collagenase مثلا ال collagenase درسناها في وين؟
+
+435
+00:32:07,240 --> 00:32:10,780
+كان .. اه في ال invasionفي ال invasion حاكيناه
+
+436
+00:32:10,780 --> 00:32:15,060
+الآن و حاكيناه و اسمه واضح ان هو عبارة عن ايه؟ عن
+
+437
+00:32:15,060 --> 00:32:20,700
+انزين بولوجينايز بيكسر لكن كونه كونه بيقتل الخلايا
+
+438
+00:32:20,700 --> 00:32:23,580
+نتيجة نتيجة ان هي بتقتل الخلايا فممكن نعتبره برضه
+
+439
+00:32:23,580 --> 00:32:28,100
+ايه؟ ممكن نعتبره toxins يعني enzymes with toxic
+
+440
+00:32:28,100 --> 00:32:32,640
+activity الاستبتاكوكاس حياليرونيديز و بيكسر
+
+441
+00:32:32,640 --> 00:32:35,220
+الهياليرونيك اسد اللي برضه بلبط الخلايا ببعضها
+
+442
+00:32:35,220 --> 00:32:42,670
+البعضmembrane-damaging-toxins شوفناهم في ال
+
+443
+00:32:42,670 --> 00:32:45,650
+animation مش بيقولنا احنا ايه بيو موديل و membrane
+
+444
+00:32:45,650 --> 00:32:49,710
+و بعدين بيقولنا super-antigens زي ال proteases
+
+445
+00:32:49,710 --> 00:32:54,970
+كلمة ال proteases هي عنوان لما احنا بيقوله برهات
+
+446
+00:32:54,970 --> 00:33:00,630
+فلوس املة بيتكلم عن دنار املة و دولار املة و شكل
+
+447
+00:33:00,630 --> 00:33:05,800
+املة و درهم املةفلكن في الآخر فيه لهم عنوان رئيسي
+
+448
+00:33:05,800 --> 00:33:09,000
+لما بيقول ال proteases هي كل الإنزيمات القادرة على
+
+449
+00:33:09,000 --> 00:33:12,180
+تكسير ال polypeptide أو ال proteinات فاهمين؟ في
+
+450
+00:33:12,180 --> 00:33:15,580
+منها أصناف متخصصة بتكون في منها أصناف متخصصة
+
+451
+00:33:15,580 --> 00:33:17,960
+phospholipases وانا حاطط بين قوسين اللي هو ال
+
+452
+00:33:17,960 --> 00:33:21,640
+acetinase اللي قولنا عنه واحد من ال phospholipases
+
+453
+00:33:21,640 --> 00:33:25,540
+وفي منهم detergent like action ايش يعني ال
+
+454
+00:33:25,540 --> 00:33:29,700
+detergent like action؟ احكي
+
+455
+00:33:37,270 --> 00:33:39,130
+وهو دي أماق يقولي احنا هنقولي اشتغلوا كال ..
+
+456
+00:33:39,130 --> 00:33:44,690
+كالمناطق تانية، نعم، بس كيف المناطق بيشتغل؟ الشكل
+
+457
+00:33:44,690 --> 00:33:49,610
+هو بدنا quiz، بدنا quiz أيش، ديك مفاجئ، عشان يحفظ
+
+458
+00:33:49,610 --> 00:33:51,630
+ال .. ال .. الذاكرة، عموما
+
+459
+00:33:55,680 --> 00:33:59,420
+كان فيه إله Hydrophilic و Hydrophobic و ال cell
+
+460
+00:33:59,420 --> 00:34:01,720
+membrane تبعنا ال phospholipids تبعتنا كانت
+
+461
+00:34:01,720 --> 00:34:04,300
+Hydrophilic و Hydrophobic ال interaction between
+
+462
+00:34:04,300 --> 00:34:07,280
+the two بيعمل destruction لمين؟ لإلمنا نفسه ما هو
+
+463
+00:34:07,280 --> 00:34:13,880
+ال detergent like action طيب ناخد واحد من الأمثلة
+
+464
+00:34:13,880 --> 00:34:16,060
+اللي هو ال eclostridium paraphringens
+
+465
+00:34:16,060 --> 00:34:19,500
+phospholipids بالمناسبة ال eclostridium
+
+466
+00:34:19,500 --> 00:34:23,830
+paraphringens مررت معانا قبل هيك تحت أي عنوانأو
+
+467
+00:34:23,830 --> 00:34:32,030
+تحت أي فكرة أو تحت أي مرض مين بتذكر؟
+
+468
+00:34:32,030 --> 00:34:34,350
+خدوا بأكوا مبررات ال quiz و قولوا مانا قاعد بالزيد
+
+469
+00:34:34,350 --> 00:34:37,370
+الفوسفول
+
+470
+00:34:37,370 --> 00:34:40,230
+.. هقولوا .. هقولوا مول مرة معانا .. لما اتكلمنا
+
+471
+00:34:40,230 --> 00:34:43,290
+عن ال endosport اتكلمنا عن ال endosport اتكلمنا
+
+472
+00:34:43,290 --> 00:34:48,410
+على twoGenera, Bacillus و Clostridium و مضمن ال
+
+473
+00:34:48,410 --> 00:34:52,610
+Clostridium تحدث عن ثلاث أصناف Perifringens و
+
+474
+00:34:52,610 --> 00:34:56,450
+Botulonium و Tetanus وقلنالكوا ال Perifringens هي
+
+475
+00:34:56,450 --> 00:35:02,190
+المسؤولة عن الجاز Gangrene الغرينة الغازية و أشهر
+
+476
+00:35:02,190 --> 00:35:08,170
+توكسين عندها هو ال phospholipase C اللي من خلالها
+
+477
+00:35:08,170 --> 00:35:14,080
+بتفكك الخلايا عن بعضها أو تقتل الخلاياطبعا خدوا
+
+478
+00:35:14,080 --> 00:35:18,480
+بالك يا بنات قتل الخلايا بيخدم غلطين انه .. انه ..
+
+479
+00:35:18,480 --> 00:35:21,840
+انه .. ال .. ال .. ال .. الخلايا اللي ماتت هتشكل
+
+480
+00:35:21,840 --> 00:35:27,280
+ايش لان؟ مادة غذائية مناسبة للبكتيريا، انت عارفين
+
+481
+00:35:27,280 --> 00:35:31,020
+على ايش بنربي احنا و بنمي الكلسترديوم؟ بنميها على
+
+482
+00:35:31,020 --> 00:35:35,940
+قطع اللحم، بنجبطيلها لحم تمام شجرة صغيرة و
+
+483
+00:35:35,940 --> 00:35:40,790
+بنغليها، اسم ال media is chopped meat brothبتحب
+
+484
+00:35:40,790 --> 00:35:43,710
+البروتينات بشكل كبير و اللحم مليان بروتينات تمام؟
+
+485
+00:35:43,710 --> 00:35:46,790
+اللحوم فلما اتنوت خلايا العضلات بتبعتها اللي هي
+
+486
+00:35:46,790 --> 00:35:50,310
+اللحم تمام هي في واقع الأول مابتصبت غرضين، الغرض
+
+487
+00:35:50,310 --> 00:35:55,310
+الأول انه بتعمل invasion، رقم اتنين بتحصل عليها
+
+488
+00:35:55,310 --> 00:35:59,570
+موال مجدية من الخلايا اللي ماتت، ال phospholipase
+
+489
+00:35:59,570 --> 00:36:02,750
+له multiple functions، it destroys blood
+
+490
+00:36:06,040 --> 00:36:09,380
+Stops influx of inflammatory cells عنده إمكانية
+
+491
+00:36:09,380 --> 00:36:14,960
+يقول الخلايا اللي جاية تعمل ردد فعل منعية، يقولها
+
+492
+00:36:14,960 --> 00:36:20,200
+أنك واقفي، ماتمش هام It helps، أنا غلطان في الجملة
+
+493
+00:36:20,200 --> 00:36:23,140
+هنا، it helps in creating an aerobic environment
+
+494
+00:36:23,140 --> 00:36:28,940
+بيساعد في خلق بيئة لا هوائي، لأن البكتيريا أجمل
+
+495
+00:36:28,940 --> 00:36:33,040
+هذه البكتيريا لا هوائي، وبالتالي هي محتاجة إلى هذه
+
+496
+00:36:33,040 --> 00:36:36,220
+البيئةallows growth of this anaerobic طبعا
+
+497
+00:36:36,220 --> 00:36:38,860
+creating anaerobic environment بيساعد على ان
+
+498
+00:36:38,860 --> 00:36:45,180
+البكتيريا تنمو في هذه البيئة اللهوة طيب
+
+499
+00:36:45,180 --> 00:36:50,420
+مازلنا في ال exotoxin ومتكلم على .. ركزوا معايا لو
+
+500
+00:36:50,420 --> 00:36:55,700
+واحد أكل طعام بيحتوي على ال botulinum toxin ايش
+
+501
+00:36:55,700 --> 00:37:01,870
+حله؟ ايش علاجه؟ان نعطيه antitoxin زى واحد جرس طوحى
+
+502
+00:37:01,870 --> 00:37:08,030
+او عقرب نعطيه antitoxin ال antitoxin شو منه ده؟
+
+503
+00:37:08,030 --> 00:37:12,330
+عبارة عن antibodies بسموها
+
+504
+00:37:12,330 --> 00:37:17,830
+neutralizing antibody ليش سموها neutralizing؟
+
+505
+00:37:20,660 --> 00:37:22,920
+فالان ال antibodies اما بيقولون عنها antibodies
+
+506
+00:37:22,920 --> 00:37:26,920
+عادية او بيقولون عنها antitoxins يعني مثلا لما
+
+507
+00:37:26,920 --> 00:37:28,740
+بيقولوا كلوستروديوم بوترونيوم antitoxin
+
+508
+00:37:28,740 --> 00:37:33,120
+الكلوستروديوم بتتانى antitoxin يبقى لما يكون ال
+
+509
+00:37:33,120 --> 00:37:39,060
+antibody مخصص للتوكسين بنسميه antitoxin وغالبا ال
+
+510
+00:37:39,060 --> 00:37:46,200
+exotoxin بنقدر نتجلها antibodyالاندوتوكسين بيكوّل
+
+511
+00:37:46,200 --> 00:37:49,700
+لها انتبالي لكن مش effective .. مش as effective as
+
+512
+00:37:49,700 --> 00:37:54,360
+اللي هو الانتوتوكسين لمين؟ للإكسودوكسين بيعطيكوا
+
+513
+00:37:54,360 --> 00:37:59,840
+أمثلة كثيرة ال ديثيريا الفاكسين اللي احنا بناخده
+
+514
+00:37:59,840 --> 00:38:03,000
+.. بناخده .. بيعطيكوا اسم الفاكسين اللي بتحفظه ..
+
+515
+00:38:03,000 --> 00:38:06,080
+انا بكتبه على الصبورة عشان نضيفه من ضمن ال ..
+
+516
+00:38:18,580 --> 00:38:28,420
+دفتيريا، هاي دفتيريا، و هاي تيتانوس، لأ بيرتوز
+
+517
+00:38:28,420 --> 00:38:28,940
+بالأول
+
+518
+00:38:49,600 --> 00:38:53,280
+تمام؟ هذا التوكسين اللي هو ال DBT هو مشهور كل جوه
+
+519
+00:38:53,280 --> 00:38:57,420
+أخدته تمام؟ بيحمينا من ال Deuterium و من ال
+
+520
+00:38:57,420 --> 00:39:00,340
+Pertosis اللي يساعدكي زي اللي هو مين اللي هو
+
+521
+00:39:00,340 --> 00:39:04,140
+اتترست التلاتة بيشتركوا بإنهم ينتجوا بينتجوا
+
+522
+00:39:04,140 --> 00:39:08,520
+Exotoxin و بناخد ال Exotoxin تبعهم و بنحوله لما
+
+523
+00:39:08,520 --> 00:39:13,480
+يسمى إلى Toxide ماربعها المصطلحات العجب لها؟ لأ،
+
+524
+00:39:13,480 --> 00:39:21,340
+توكسين بتحول لإيه؟ Toxide لبنقول Oidإيش معناها؟
+
+525
+00:39:21,340 --> 00:39:29,120
+Oid، metal، metalloid، alkaline، alkaloid، شبيه
+
+526
+00:39:29,120 --> 00:39:33,480
+بيه، شبيه بيه، metal، metalloid، إيش بقى؟ Toxin،
+
+527
+00:39:33,480 --> 00:39:35,700
+toxoid، خليكم معايا برنامج تنفذوا معايا في الجزئية
+
+528
+00:39:35,700 --> 00:39:42,230
+هذهإذا هالتوكسين إيه له صفتين بدنا نركز عليهم صفة
+
+529
+00:39:42,230 --> 00:39:46,590
+أولى ال toxicgenicity إيه ال toxicitygenicity؟
+
+530
+00:39:46,590 --> 00:39:53,410
+السمية والصفة التانية ال antigenicity انه لو دخل
+
+531
+00:39:53,410 --> 00:39:57,170
+اسم الإنسان اسم الإنسان بيكوّله أسام مضادة احنا
+
+532
+00:39:57,170 --> 00:40:02,450
+مين اللي بيدينا؟ ال toxicgenic فبنروح و احنا ..
+
+533
+00:40:02,450 --> 00:40:09,020
+يعني لو نعملها بشكل خياليالطرفين هذا ال toxic part
+
+534
+00:40:09,020 --> 00:40:14,400
+و هذا ايش قلناه اللي هو ال antigenic part لو جسينا
+
+535
+00:40:14,400 --> 00:40:19,420
+ال toxigenic و خلينا بس ال antigenic ودخلنا هذا ال
+
+536
+00:40:19,420 --> 00:40:23,340
+antigen او هذا ال toxin الى دسم الحيوان، دسم
+
+537
+00:40:23,340 --> 00:40:27,140
+الحيوان بيكوله عسان مضاد دون ان يتأثر ان ال
+
+538
+00:40:27,140 --> 00:40:31,730
+toxigen استشمالها راحبناخد ال antibodies الجاهزة
+
+539
+00:40:31,730 --> 00:40:36,330
+هذه و بتصير أنها مصدر لو انزلجنا بدنا نعطي مريض
+
+540
+00:40:36,330 --> 00:40:40,750
+antibodies نعطيه ايه؟ امان؟ طيب احنا بدنا نضلنا
+
+541
+00:40:40,750 --> 00:40:43,330
+نصنع فيه الحيوانات و الحيوانات بدنا نصفي ال
+
+542
+00:40:43,330 --> 00:40:46,810
+antibodies و قد يبقى بقايا لو حقنها في إنسان كونه
+
+543
+00:40:46,810 --> 00:40:50,830
+غريب ممكن ال serum تبقى الحيوان يؤذي مين؟ الإنسان
+
+544
+00:40:50,830 --> 00:40:57,360
+فلماذا نقوم بإعطاء المريض نفسه تطيب محتوي علىعال
+
+545
+00:40:57,360 --> 00:41:01,460
+.. على الtoxoid اللي احنا هو يبارح عن تكسين جسنا
+
+546
+00:41:01,460 --> 00:41:05,680
+منه او خربنا فيه اللي هو ال toxicgenic part دون
+
+547
+00:41:05,680 --> 00:41:09,720
+انه اثر على مين؟ على ال antigenic part وسط الصورة
+
+548
+00:41:09,720 --> 00:41:13,840
+هاي ال toxoid، ال toxoid يبارح عن تكسين تم تدمير
+
+549
+00:41:13,840 --> 00:41:18,660
+ال toxicgenic side تبعه وهذا هو اللي انتوا أخدتوا
+
+550
+00:41:18,660 --> 00:41:21,440
+.. لما أخدتوا ال diphtheria و ال pertussis و ايه؟
+
+551
+00:41:21,440 --> 00:41:27,340
+ال تتانوس اللي هو ال DPDإذا بنقدر نستنتج من اللوحة
+
+552
+00:41:27,340 --> 00:41:30,860
+دي اللي بدأنا نتكلم عنها استنتاج مهم جدا ان ال
+
+553
+00:41:30,860 --> 00:41:35,740
+exotoxins يمكن ال neutralization تبعتها بمين ب ..
+
+554
+00:41:35,740 --> 00:41:41,160
+ب .. بواسطة ال antitoxin هو يمكن لو انا اتناولت
+
+555
+00:41:41,160 --> 00:41:45,240
+الغذاء انا عندي توكسين الآن دخل مش بده يجيني بعد
+
+556
+00:41:45,240 --> 00:41:48,540
+سنة او سنتين الآن دخل فعشان انا عاجز اني معالجه ب
+
+557
+00:41:48,540 --> 00:41:49,580
+antitoxin
+
+558
+00:42:09,380 --> 00:42:13,880
+الشخص اللي أكل الطعام من فاسد هيكوسيرتوكسين،
+
+559
+00:42:13,880 --> 00:42:17,600
+فالناس جاهزة منها على هذا، يعني لإنه تلقائي بدون
+
+560
+00:42:17,600 --> 00:42:23,420
+إشكالجهاز المناعة إذا كان بيقدر يتعامل مع
+
+561
+00:42:23,420 --> 00:42:27,980
+المراكبات بشكل عام بيختلف، يعني انت لاحظ شغلة، في
+
+562
+00:42:27,980 --> 00:42:31,620
+سموم أقوى من سموم، مظبوط ولا لأ؟ لماذا سموم أقوى
+
+563
+00:42:31,620 --> 00:42:34,640
+من سموم؟ في سموم تشتغل على النار على طول وانتهى من
+
+564
+00:42:34,640 --> 00:42:39,920
+الموضوع، جهاز المناعة بالحاجش حتى يفكر، طبعا؟ وفي
+
+565
+00:42:39,920 --> 00:42:44,080
+توكسينز بتكون بطيقة ممكن تنبه جهاز المناعة
+
+566
+00:42:44,080 --> 00:42:48,600
+وبالتالي يكون في action لها، فأنا بعطي شغل تاني
+
+567
+00:42:49,470 --> 00:42:53,130
+بعطي muscle .. muscle يعني إيه؟ antitoxin جاهز برا
+
+568
+00:42:53,130 --> 00:42:58,970
+أو بعطي vaccine .. vaccine لمين؟ تخيل واحد زاكيتنا
+
+569
+00:42:58,970 --> 00:43:04,430
+ماعرفش أهتجاج في ال .. في خدنا نقول في العمل في
+
+570
+00:43:04,430 --> 00:43:07,710
+الميدان، مسامير وغيره وغيرته، يعني أقل أو أفضل من
+
+571
+00:43:07,710 --> 00:43:12,370
+الآخر، لكن في حياته معرض أن ينصف مصمار أو مصمرين
+
+572
+00:43:12,370 --> 00:43:18,220
+ولا أداة جرح من هنا أو من هناك، إيش بناعطيه؟عنطي
+
+573
+00:43:18,220 --> 00:43:22,600
+تكسيد اللي هو تكسين مضعف، فجسمنا بيكون كوّن أجسام
+
+574
+00:43:22,600 --> 00:43:27,760
+مضادة، فيما لو دخل التكسين حقا، جسمنا يقوم
+
+575
+00:43:27,760 --> 00:43:31,980
+بانجازله، بس لو حصل و أخدنا تكساء التكسين،
+
+576
+00:43:31,980 --> 00:43:35,600
+تناولنا، تمام؟ مافيش لنا حل إلا إنه ناخد عنطي
+
+577
+00:43:35,600 --> 00:43:36,360
+تكسيد
+
+578
+00:43:44,230 --> 00:43:48,350
+نجلع اللوحة المتعلقة بالاندوتوكسين و نطلع على
+
+579
+00:43:48,350 --> 00:43:54,810
+الرسم اللي موجودة هيصرف معروضة أمامكم بأشكال و
+
+580
+00:43:54,810 --> 00:43:58,850
+ألوان مختلفة منذ أن بدأنا بالحديث عن الاندوتوكسين
+
+581
+00:43:58,850 --> 00:44:03,010
+في النهاية هي مكوّنة من ثلاث أجزاء lipid-A,
+
+582
+00:44:03,350 --> 00:44:09,050
+corpulisicle, and oocyte chain الاندوتوكسين
+
+583
+00:44:09,050 --> 00:44:11,910
+هو ال lipid-Aمش مانع الحاجة .. مش مانع الحاجة ان
+
+584
+00:44:11,910 --> 00:44:15,510
+هو الأهم، بس احنا لإننا بنتكلم عن ال toxins تمام،
+
+585
+00:44:15,510 --> 00:44:24,750
+بنتكلم عن ال lipid A، ال lipid A اللي
+
+586
+00:44:24,750 --> 00:44:30,530
+سمناه كامرة لل endotoxinطبعا عشان يصيرله release
+
+587
+00:44:30,530 --> 00:44:34,410
+ال cell wall لازم يصيرله تحلل if it is required
+
+588
+00:44:34,410 --> 00:44:38,910
+for its release that the cell wall is lysed ممكن
+
+589
+00:44:38,910 --> 00:44:42,450
+نوصفه هذا ال .. ال .. ال .. ال library و ال .. انه
+
+590
+00:44:42,450 --> 00:44:46,490
+formaldehyde and heat resistant يعني فينا نوع من
+
+591
+00:44:46,490 --> 00:44:50,110
+ايه؟ من مقاومة لل formaldehyde ليش ال formaldehyde
+
+592
+00:44:50,110 --> 00:44:53,170
+بالذات؟لأن الformaldehyde هو اللي استخدمناه يا
+
+593
+00:44:53,170 --> 00:44:58,930
+بنات لتحويل ال toxin .. ال exotoxin ل toxoid يبقى
+
+594
+00:44:58,930 --> 00:45:04,850
+ال exotoxin حولت إلى toxoid بواسطة مين يبقى ال
+
+595
+00:45:04,850 --> 00:45:08,190
+formaldehyde عمل destruction لل toxic side لكن
+
+596
+00:45:08,190 --> 00:45:11,390
+للأسف لما حطيناها على ال endoxin ماقدرنا .. كنا
+
+597
+00:45:11,390 --> 00:45:15,590
+بننقل نفس الفكرة أننا نعمل toxoid من ال endoxin
+
+598
+00:45:15,590 --> 00:45:20,050
+ماقدرناماجدرناش تمام؟ فإن ال formal diet and heat
+
+599
+00:45:20,050 --> 00:45:23,550
+resistant poor antigen يعني و هذا كمان أحد نقاط
+
+600
+00:45:23,550 --> 00:45:29,150
+قوته و نقاط ضعفه نقاط قوة للبكتيريا إن الجسم لا
+
+601
+00:45:29,150 --> 00:45:33,690
+بيكوّلوش أجسام مضغطة بشكل قوي وبتالي مابتقدرش ..
+
+602
+00:45:33,690 --> 00:45:38,210
+مابتقدرش نقاوم البكتيريا من خلالها as a free
+
+603
+00:45:38,210 --> 00:45:41,790
+molecule هذا اليوم ماعرفش يعني .. المرة أنا ممكن
+
+604
+00:45:41,790 --> 00:45:44,650
+أبسلها و لا .. لو .. لو .. لو .. لو رابطنا .. لو
+
+605
+00:45:44,650 --> 00:45:49,270
+رابطنا ب .. بحدة تانية يعنيخلّيكم معاه، هي
+
+606
+00:45:49,270 --> 00:45:53,130
+المُركّب اللي هو ال intoxant، ك free molecule .. ك
+
+607
+00:45:53,130 --> 00:45:57,410
+free molecule، إيش فيه أنه فيه؟ فيه أنه poor
+
+608
+00:45:57,410 --> 00:46:00,630
+antigenicity، لكن أنا بنعت فيه في القلم المناع
+
+609
+00:46:00,630 --> 00:46:04,430
+اسمه adjuvant، adjuvant، لو ركّبنا هذا ال .. ال
+
+610
+00:46:04,430 --> 00:46:07,550
+molecule على ال adjuvant، بصير فيه نوع من ال ..
+
+611
+00:46:07,550 --> 00:46:11,210
+إيه؟ من ال response الأفضل، لكن لن يصل بأي حال من
+
+612
+00:46:11,210 --> 00:46:14,990
+الأحوال لل response تبع مين؟ تبع ال exotoxins اللي
+
+613
+00:46:14,990 --> 00:46:18,380
+شوفناها في التكسيراتنكمل ان شاء الله المرة القادمة
+
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/SVYwdKMMVYg.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/SVYwdKMMVYg.srt
new file mode 100644
index 0000000000000000000000000000000000000000..98b9da5b81f5f32a2fb25fe29ff08e157d36124e
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/SVYwdKMMVYg.srt
@@ -0,0 +1,2631 @@
+1
+00:00:21,060 --> 00:00:23,860
+بسم الله الرحمن الرحيم اليوم إن شاء الله هنكمل مع
+
+2
+00:00:23,860 --> 00:00:30,820
+بعض تكملة لـ الـ antibiotics الجزء 
+
+3
+00:00:30,820 --> 00:00:33,640
+السفلي هذا هو الـ peptidoglycan يعني اللي بتكون من
+
+4
+00:00:33,640 --> 00:00:36,980
+NAM و NAG و بينهم glycosidic bond و بين الـ NAM و
+
+5
+00:00:36,980 --> 00:00:40,000
+الـ NAG في صفقة و طبقة فيها اللي هو الـ cross link
+
+6
+00:00:40,000 --> 00:00:43,900
+هذا الـ cell membrane بيتكون طبعًا البكتيريا بتنمو و
+
+7
+00:00:43,900 --> 00:00:47,180
+تكاثر بذات استطيل فبالتالي بتكون محتاجة جديدة من
+
+8
+00:00:47,180 --> 00:00:52,960
+مين؟ من الـ peptidoglycan تكونات واحدة في آنٍ حاسم
+
+9
+00:00:52,960 --> 00:00:56,720
+أوتوليسين مرة بمعناها قبل هيك بتقص الـ glycosidic
+
+10
+00:00:56,720 --> 00:01:01,740
+bond اللي بتلاحظه الآن هتفرق عشان يصير في آنٍ
+
+11
+00:01:01,740 --> 00:01:05,260
+واحدة incorporation لـ new units صار في آنٍ
+
+12
+00:01:05,260 --> 00:01:08,600
+incorporation لـ units لكن ما صار لسه فيه binding
+
+13
+00:01:08,600 --> 00:01:13,380
+ففي آنٍ two two things مهمين جدًا إنه يتكون
+
+14
+00:01:13,380 --> 00:01:16,600
+glycosidic bond على الـ NAM والـ NAG ويتكون cross
+
+15
+00:01:16,600 --> 00:01:19,800
+link بين الاتنين فواحد اسمه إنزيم
+
+16
+00:01:22,570 --> 00:01:26,730
+Transpeptidase اللي بيكون لـ cross-link و Trans
+
+17
+00:01:26,730 --> 00:01:32,930
+Glycosidase اللي بيكون لـ glycosidic bond الآن في
+
+18
+00:01:32,930 --> 00:01:37,910
+أن هذا الـ normal قلنا إيه؟ normal synthesis
+
+19
+00:01:37,910 --> 00:01:41,370
+للـ peptidoglycan بتعيدوا كمان مرة قبل ما ننتقل
+
+20
+00:01:41,370 --> 00:01:46,030
+إلى الـ antibiotic اللي بتأثر على الـ peptidoglycan
+
+21
+00:01:46,030 --> 00:01:50,390
+مناسبة الـ membrane بيحصل في تصنيع الـ units اللي هي
+
+22
+00:01:50,390 --> 00:01:56,230
+الـ NAM والـ NAG بس بيحصل transport إلى الـ biological
+
+23
+00:01:56,230 --> 00:02:01,970
+lab الاوتولاسين بيشتغل بيعمل كسر عشان يوسع و يدخل
+
+24
+00:02:01,970 --> 00:02:06,590
+يعمل incorporation لـ new units لذلك بنلاقي أن الـ
+
+25
+00:02:06,590 --> 00:02:09,590
+antibiotics ما اللي بتأثر على الـ cell wall ما
+
+26
+00:02:09,590 --> 00:02:12,090
+بتأثر على الـ fully grown bacteria بتأثر فقط على
+
+27
+00:02:12,090 --> 00:02:16,450
+تلك اللتي تم قسمها أما اللي already grown ما بتأثر
+
+28
+00:02:16,450 --> 00:02:19,880
+أنه مافيها biological synthesis صار في
+
+29
+00:02:19,880 --> 00:02:23,820
+incorporation useless إذا ما صار فيه bond ما بين
+
+30
+00:02:23,820 --> 00:02:26,960
+الجديد والجديد وما صار فيه cross-link هذا useless
+
+31
+00:02:26,960 --> 00:02:33,000
+فبتعرفوا أنها قلنا الـ glycosidic bond وبعدين
+
+32
+00:02:33,000 --> 00:02:35,580
+اللي هو الـ Transpeptidase بيعمل اللي هو الـ cross
+
+33
+00:02:35,580 --> 00:02:38,280
+-link كده هذه الجزء الأول اللي هو الـ normal
+
+34
+00:02:38,280 --> 00:02:42,580
+synthesis of peptidoglycan نشوفه مرتين، هنشوفه
+
+35
+00:02:42,580 --> 00:02:45,640
+كمان مرة تعالى نشوف الـ penicillin
+
+36
+00:02:52,770 --> 00:03:00,930
+نفس النظام ستجد أن الخطوات تبعت الـ normal
+
+37
+00:03:00,930 --> 00:03:04,770
+synthesis شغالة الفرق بين الأولى والثانية أن الآن
+
+38
+00:03:04,770 --> 00:03:09,410
+هناك الـ penicillin البنسلين بيشتغل، هيشتغل على الـ
+
+39
+00:03:09,410 --> 00:03:11,230
+cell wall صحيح لكن و لا بالـ cell wall بالظبط
+
+40
+00:03:11,230 --> 00:03:14,350
+هيشتغل على الـ Transpeptidase زي ما هتشوفوا الآن
+
+41
+00:03:14,350 --> 00:03:17,510
+هيعمل Blocking إيش هي الـ Blocking؟ يعني هيجيب الـ
+
+42
+00:03:17,510 --> 00:03:20,130
+Transpeptidase و يرتبط معاه يقول له أنت الآن مالكاش
+
+43
+00:03:20,130 --> 00:03:24,370
+شغل يبقى مش هتكون cross link و إحنا متفقين منذ
+
+44
+00:03:24,370 --> 00:03:28,990
+بداية الحديث عن الـ cell structure لما اتكلمنا أن
+
+45
+00:03:28,990 --> 00:03:32,130
+خلية بدون cross link أو cell wall بدون cross link
+
+46
+00:03:32,130 --> 00:03:35,910
+هو cell wall يعني ضعيف جدًا تشوفوا الآن هذه العملية
+
+47
+00:03:35,910 --> 00:03:40,480
+اللي تمت، اتكونت الـ glycosidic bond بالـ
+
+48
+00:03:40,480 --> 00:03:44,400
+Transglycosidase لكن ما اتكون اللي هو الـ cross
+
+49
+00:03:44,400 --> 00:03:48,840
+link إذا الآن بما يتكون بالـ Transpeptidase لحظوا
+
+50
+00:03:48,840 --> 00:03:52,320
+هاي الـ Transpeptidase إيجي الـ penicillin اللي بيقولوا
+
+51
+00:03:52,320 --> 00:03:56,740
+البنفسجي هيرتبط بمين؟ بالـ Transpeptidase يعني مافيش
+
+52
+00:03:56,740 --> 00:04:00,660
+إمكانية الآن إنه يتكون cross link متفقين عليها
+
+53
+00:04:00,660 --> 00:04:02,080
+النقطة
+
+54
+00:04:07,670 --> 00:04:11,670
+بالـ Transpeptidase ليش مهم الكلام هذا؟ لأنه هنشوف
+
+55
+00:04:11,670 --> 00:04:16,250
+واحد ثاني الآن اللي هو الـ Vancomycin و الـ
+
+56
+00:04:16,250 --> 00:04:18,490
+Vancomycin بيؤدي لنفس النتيجة مش هتكون cross
+
+57
+00:04:18,490 --> 00:04:27,370
+link لكن بطريقة مختلفة يعني
+
+58
+00:04:27,370 --> 00:04:30,510
+المفروض الآن لسه الـ word synthesis صار لنا واضح هي
+
+59
+00:04:30,510 --> 00:04:38,660
+ثالث مرة بنكرره تمام؟ كمان عندنا الآن؟ الـ Units
+
+60
+00:04:38,660 --> 00:04:50,560
+Transport الـ Autolysin هيشتغل يفسح مكان قدام
+
+61
+00:04:50,560 --> 00:04:53,680
+حضرت مين الآن؟ الـ Vancomycin اللي هو عبارة عن الـ
+
+62
+00:04:53,680 --> 00:04:59,580
+Glycopeptide ارتبط بالـ Sites اللي بيشتغل عليها
+
+63
+00:04:59,580 --> 00:05:02,360
+مين اللي بيشتغل عليها؟ الـ Transpeptidase
+
+64
+00:05:06,820 --> 00:05:10,580
+و الأصل الـ Transpeptidase بيشبك هنا و بيكون الـ
+
+65
+00:05:10,580 --> 00:05:13,420
+cross link لأن بيجي الـ Transpeptidase بده يشتغل
+
+66
+00:05:13,420 --> 00:05:17,880
+بلا جيش مكانه فارغ وبالتالي لن يستطيع أن يكون
+
+67
+00:05:17,880 --> 00:05:21,700
+cross link إذا في المحصلة الـ Vancomycin و الـ
+
+68
+00:05:21,700 --> 00:05:27,680
+Vancomycin كلاهما أشملهم مانعوا الـ cross link لكن
+
+69
+00:05:27,680 --> 00:05:30,980
+واحد عبر طريق الـ blocking لـ Transpeptidase و واحد
+
+70
+00:05:30,980 --> 00:05:32,360
+blocking للـ site
+
+71
+00:05:36,380 --> 00:05:43,160
+لكن في المحصلة دلوقتي هما بيأثروا على الـ cell wall
+
+72
+00:05:45,280 --> 00:05:48,080
+طيب أنا قلت لكم المحاضرة الماضية إننا بنعمل بعض
+
+73
+00:05:48,080 --> 00:05:53,240
+أحيانًا modification صح؟ للـ antibiotic to extend the
+
+74
+00:05:53,240 --> 00:05:56,960
+spectrum ومثال الـ penicillin صار يؤثر على الـ gram
+
+75
+00:05:56,960 --> 00:05:59,820
+positive ويؤثر على الـ gram negative والـ penicillin
+
+76
+00:05:59,820 --> 00:06:03,780
+أخوه الـ penicillin هل تأثير الـ .. أو الـ mechanism of
+
+77
+00:06:03,780 --> 00:06:07,080
+action تبع الـ penicillin على الـ gram negative هو نفسه
+
+78
+00:06:07,080 --> 00:06:11,220
+تأثيره على الـ gram positive بالتأكيد لا، يبقى على
+
+79
+00:06:11,220 --> 00:06:14,340
+جرام نيجاتيف كيف بده يشتغل؟ بيشتغل على حالة اسمها
+
+80
+00:06:14,340 --> 00:06:17,000
+Penicillin Binding Proteins فهناك مجموعة من
+
+81
+00:06:17,000 --> 00:06:21,140
+البروتينات مهمة جدًا إيه العلاقات اللي بتصنعها الـ
+
+82
+00:06:21,140 --> 00:06:25,880
+peptidoglycan والـ cell wall فإذا كان الـ Penicillin
+
+83
+00:06:25,880 --> 00:06:28,620
+Binding Proteins اللي ارتبطت بالـ Penicillin فبيصير
+
+84
+00:06:28,620 --> 00:06:32,100
+تكسير لمين؟ للـ cell wall تبع البكتيريا بطريقة
+
+85
+00:06:32,100 --> 00:06:37,860
+مختلفة عما حدث في الـ gram نرجع
+
+86
+00:06:37,860 --> 00:06:44,500
+للـ presentation و نذكركم لما وصلنا إحنا فاكرين
+
+87
+00:06:44,500 --> 00:06:48,590
+هذه؟ ختمنا فيها إحنا المرة الماضية المحاضرة تبعتنا
+
+88
+00:06:48,590 --> 00:06:52,930
+لما قلنا أن الـ .. الـ beta lactam ring اللي هي
+
+89
+00:06:52,930 --> 00:06:57,450
+الجزء الأساسي من الـ penicillin و من الـ cephalosporins
+
+90
+00:06:57,450 --> 00:07:02,090
+تمام؟ و لذلك سميناها الـ cephalosporins و الـ .. الـ
+
+91
+00:07:02,090 --> 00:07:05,730
+.. الـ penicillin سميناها beta lactam antibiotic إذا
+
+92
+00:07:05,730 --> 00:07:10,270
+صار فيه تكسير الـ ring تبعت الـ beta lactam صار الـ
+
+93
+00:07:10,270 --> 00:07:12,470
+antibiotic اللي اسمه penicillin صار اسمه penicillin
+
+94
+00:07:12,470 --> 00:07:15,170
+acid و فقد أي activity أو أي antimicrobial
+
+95
+00:07:15,170 --> 00:07:15,750
+activity
+
+96
+00:07:18,390 --> 00:07:21,110
+هو آخر من الـ .. من الـ antibiotics اللي أثرت سلوك
+
+97
+00:07:21,110 --> 00:07:23,730
+لها وزن كبير جدًا في الـ antibiotics اسمها
+
+98
+00:07:23,730 --> 00:07:28,130
+cephalosporins، cephalosporins الـ cephalosporins
+
+99
+00:07:28,130 --> 00:07:33,170
+أول ما اكتشفت لُجِئوا أن التأثير تبعها يشبه إلى حد
+
+100
+00:07:33,170 --> 00:07:37,010
+كبير تأثير الـ penicillin، و هما الاثنين إحنا قلنا معاهم
+
+101
+00:07:37,010 --> 00:07:38,770
+beta lactam-antibiotics
+
+102
+00:07:40,940 --> 00:07:44,280
+لما عملنا modification لهم وجدنا أن لا ممكن
+
+103
+00:07:44,280 --> 00:07:47,880
+تأثيرهم يمتد إلى جرام نيجاتيف، لاحظوا الآن في
+
+104
+00:07:47,880 --> 00:07:53,720
+أن second, third and fourth generations، إيش
+
+105
+00:07:53,720 --> 00:07:56,380
+يعني second و third و fourth generation؟ أنتم
+
+106
+00:07:56,380 --> 00:08:00,850
+معكم موبايلات اليوم اللي هي الـ smart هذه؟ في قبلها
+
+107
+00:08:00,850 --> 00:08:06,710
+كان موبايلات مش smart لجانب كاميرا و GPS قبلها كان
+
+108
+00:08:06,710 --> 00:08:11,150
+مش عارف فيه أقل جودة فلما يكون فيه نقلة نوعية في
+
+109
+00:08:11,150 --> 00:08:15,610
+الحواسيب في الـ antibiotics في الجوالات بنسميهم
+
+110
+00:08:15,610 --> 00:08:18,670
+أجيال، الجيل الأول، الجيل الثالث فالـ first
+
+111
+00:08:18,670 --> 00:08:21,270
+generation اللي هو الـ natural الـ first generation
+
+112
+00:08:21,270 --> 00:08:24,870
+اللي هو الـ natural هذا بيأثر على الـ gram mostly
+
+113
+00:08:26,960 --> 00:08:30,520
+بيؤثر على كلاهما لكن تأثيرهم على الـ gram negative
+
+114
+00:08:30,520 --> 00:08:34,900
+أفضل من اللي على الـ gram positive وهنا يبقى نُوّت إلى أن
+
+115
+00:08:34,900 --> 00:08:37,760
+الـ cephalosporin الثالث والرابع generation لهم
+
+116
+00:08:37,760 --> 00:08:41,580
+activity رائعة جدًا على الـ Pseudomonas aeruginosa
+
+117
+00:08:42,470 --> 00:08:45,690
+بمعنى آخر إحنا بنفتخر لما نلاقي antibiotic له
+
+118
+00:08:45,690 --> 00:08:51,190
+تأثير على Pseudomonas aeruginosa لما لها من خبرة
+
+119
+00:08:51,190 --> 00:08:54,570
+سابقة إحنا بنعرفها naturally resistance للبكتيريا
+
+120
+00:08:54,570 --> 00:08:57,570
+للـ antibiotic و can acquire resistance فبالتالي
+
+121
+00:08:57,570 --> 00:09:00,910
+لما نلاقي antibiotic effective عليها بنكون سعداء
+
+122
+00:09:00,910 --> 00:09:04,270
+جدا من ضمن هذا الـ antibiotic الـ third والـ fourth
+
+123
+00:09:04,270 --> 00:09:06,330
+generation cephalosporins
+
+124
+00:09:11,560 --> 00:09:15,900
+أصبح إنه resistance عالي ضد هذه الـ antibiotics
+
+125
+00:09:15,900 --> 00:09:21,680
+بتتلاحظوا الـ beta-lactam اللي موجودة هنا نفسها هي
+
+126
+00:09:21,680 --> 00:09:24,560
+الـ beta-lactam اللي موجودة هنا هذه عبارة عن
+
+127
+00:09:24,560 --> 00:09:28,500
+cephalosporin nucleus وهذه penicillin nucleus
+
+128
+00:09:28,500 --> 00:09:28,900
+تمام؟
+
+129
+00:09:34,170 --> 00:09:36,790
+اللي بتكسر الـ beta-lactam ممكن نقول
+
+130
+00:09:36,790 --> 00:09:40,370
+cephalosporinase عنها إذا كانت بتستهدف الـ
+
+131
+00:09:40,370 --> 00:09:43,790
+cephalosporin أو نقول penicillinase إذا بتستهدف
+
+132
+00:09:43,790 --> 00:09:48,130
+الـ penicillin أو كلا هما بيسميها وهذا الأسلم
+
+133
+00:09:48,130 --> 00:09:50,810
+الصحيح نسميها beta-lactamases
+
+134
+00:09:54,670 --> 00:09:56,530
+من الـ Antibiotics الأخرى اللي بتأثر على الـ cell
+
+135
+00:09:56,530 --> 00:09:59,370
+wall، Antibiotic مشهور بتعرفوه أنتم اسمه
+
+136
+00:09:59,370 --> 00:10:04,690
+Bacitracin هذا الـ Bacitracin very toxic ما بنقدرش 
+
+137
+00:10:04,690 --> 00:10:08,930
+نستخدمه systemic يعني ناخده حبوب أو ناخده
+
+138
+00:10:08,930 --> 00:10:12,790
+injection فبيستخدم فقط topical، إذا الـ Bacitracin
+
+139
+00:10:12,790 --> 00:10:15,990
+بيستخدم إيه؟ إيش يعني topical؟ يعني قطرة يعني موضعي
+
+140
+00:10:18,890 --> 00:10:21,950
+it is used against gram positive ولاحظوا كل الـ
+
+141
+00:10:21,950 --> 00:10:25,010
+antibiotics اللي بتأثر على الـ cell wall غالبا
+
+142
+00:10:25,010 --> 00:10:28,630
+أساسا على مين؟ على gram positive لأن هو الفعل ..
+
+143
+00:10:28,630 --> 00:10:31,590
+الموضوع الـ cross link بالظبط مهم جدا له في هذا
+
+144
+00:10:31,590 --> 00:10:34,810
+الحالة الـ vancomycin حكينا وشفناها وهو عبارة عن
+
+145
+00:10:34,810 --> 00:10:37,050
+الـ glycopeptide وأنا قد كل مرة قلت لكم أن هو the
+
+146
+00:10:37,050 --> 00:10:40,250
+last bullet هو الموضوع اللي مكتوب كل مرة the last
+
+147
+00:10:40,250 --> 00:10:45,380
+line against antibiotic resistant stuff اللي أنا
+
+148
+00:10:45,380 --> 00:10:48,580
+قلت لكم من المرة الماضية أنه لأ للبطالة الاصطناعي
+
+149
+00:10:48,580 --> 00:10:53,060
+لأنه أصلا لجينا البنكومايسين intermediate أستفيد
+
+150
+00:10:53,060 --> 00:10:55,180
+الفوكاس أورس ولجينا البنكومايسين resistant
+
+151
+00:10:55,180 --> 00:11:01,610
+أستفيد الفوكاس أورس فهنشوف لازم نركز على بدأطيب
+
+152
+00:11:01,610 --> 00:11:05,190
+في كمان two drugs بيعتبروهم cell wall inhibitors
+
+153
+00:11:05,190 --> 00:11:09,390
+لكن ليس لهم علاقة بذاكر البكتيريا فقط بنوع آخر من
+
+154
+00:11:09,390 --> 00:11:14,290
+البكتيريا اللي هي البكتيريا المسمى mycobacteria
+
+155
+00:11:14,290 --> 00:11:19,090
+لذلك سميناهم Anti Mycobacterium Antibiotics ليش
+
+156
+00:11:19,090 --> 00:11:22,890
+خصصنا Anti Mycobacterium لأن الحقيقة ثانية دولة
+
+157
+00:11:22,890 --> 00:11:24,810
+استخدموهم فقط مقصود
+
+158
+00:11:28,500 --> 00:11:32,360
+على بكتيريا أخرى غير اللي هو الـ micro bacteria
+
+159
+00:11:32,360 --> 00:11:36,360
+يعني مثلا الـ streptomycin معروف عنه إنه له تأثير على
+
+160
+00:11:36,360 --> 00:11:39,020
+الـ micro bacteria وعلى other bacteria لكن
+
+161
+00:11:39,020 --> 00:11:42,240
+ما سميناه antimicrobacterium antibacterium لماذا؟
+
+162
+00:11:42,240 --> 00:11:48,020
+لأنه مش exclusive بسبب تنين exclusive وأذكركم
+
+163
+00:11:48,020 --> 00:11:53,400
+فيهم أنا بشكل سريع لأن روحنا نطلع على اللوحة اللي
+
+164
+00:11:53,400 --> 00:11:55,220
+فيها الـ broad spectrum والـ neuroscience
+
+165
+00:11:57,900 --> 00:12:01,080
+الإيزونيازايد اللي .. اللي الآن نتكلم عنه احنا هو
+
+166
+00:12:01,080 --> 00:12:04,240
+اللي موجود تحت الـ microbacterium بينما الـ
+
+167
+00:12:04,240 --> 00:12:06,960
+streptomycin هو على microbacterium وعلى gram negative
+
+168
+00:12:06,960 --> 00:12:11,980
+فما بيعملوا antimicrobacterium فقط تلك اللي مخصصة
+
+169
+00:12:11,980 --> 00:12:17,940
+exclusive للنوع هذا نرجع برضو ونشوف الـ
+
+170
+00:12:17,940 --> 00:12:21,400
+antimicrobacterium اللي هم الثانية الإيزونيازايد
+
+171
+00:12:25,160 --> 00:12:29,200
+الذي أحدث ثورة في علاج الـ micro bacterium
+
+172
+00:12:29,200 --> 00:12:33,760
+tuberculosis قبل اكتشاف هذا الـ antibiotic كان علاج
+
+173
+00:12:33,760 --> 00:12:37,960
+الـ tuberculosis تقريبا شبه مستحيل أو يستغرق ثلث
+
+174
+00:12:37,960 --> 00:12:41,700
+سنوات أو أربع سنوات بالـ antibiotics الأخرى لما تم
+
+175
+00:12:41,700 --> 00:12:46,740
+اكتشاف هذا الـ antibiotic وصلنا لمرحلة إنه نعالج الـ
+
+176
+00:12:46,740 --> 00:12:50,520
+tuberculosis في ستة أشهر، في ستة أشهر، هذا هو
+
+177
+00:12:50,520 --> 00:12:55,080
+الرقم القياسي للـ mycobacterium tuberculosis هذا يعني
+
+178
+00:12:55,080 --> 00:12:57,680
+إنها إينهيبيشن لـ mycolic acid، وكلّكم بتعرفوا أن
+
+179
+00:12:57,680 --> 00:13:01,820
+البكتيريا تتميز بـ waxy lipids اللي هو الـ mycolic
+
+180
+00:13:01,820 --> 00:13:06,000
+acid بدونه هي إيه تتميز بشيء، وبالتالي هذا الـ
+
+181
+00:13:06,000 --> 00:13:09,460
+antibiotic منع تصنيع الـ mycolic acid وجهة منع الـ
+
+182
+00:13:09,460 --> 00:13:14,250
+replication تبعه الثاني الـ ethambutol بمنعش تصنيع
+
+183
+00:13:14,250 --> 00:13:17,770
+الـ mycolic acid لكن بيمنع الـ incorporation تبعه،
+
+184
+00:13:17,770 --> 00:13:20,770
+إيش incorporation؟ يعني أنا هاي الـ mycolic acid
+
+185
+00:13:20,770 --> 00:13:24,510
+صنعته، لازم أركبه في مكانه، هان بيمنع تركيب الـ
+
+186
+00:13:24,510 --> 00:13:27,390
+mycolic acid في مكانه، وفي المحصلة النهائية
+
+187
+00:13:27,390 --> 00:13:33,250
+الثانية ما فيش mycolic acid، ما فيش mycolic acid نجي
+
+188
+00:13:33,250 --> 00:13:37,510
+لنّوع ثاني من الـ drugs اللي هم الـ الـ الـ protein
+
+189
+00:13:37,510 --> 00:13:40,370
+synthesis inhibitors ويا ريت قبل ما نطلع على
+
+190
+00:13:40,370 --> 00:13:43,830
+اللوحة بنحاول نعرف برضو بعض اللي هو الـ animation
+
+191
+00:13:43,830 --> 00:13:44,810
+كتير مهمين
+
+192
+00:14:10,730 --> 00:14:14,470
+ننظر لو هنشيرها للآن، عندنا اللي هو الـ .. الـ
+
+193
+00:14:14,470 --> 00:14:18,910
+macrolides الـ macrolides هي مجموعة chemical group
+
+194
+00:14:18,910 --> 00:14:22,530
+من الـ antibiotics تشمل مجموعة من الـ .. من الـ
+
+195
+00:14:22,530 --> 00:14:25,570
+antibiotics برضو المهمة زي الاريثروميسين،
+
+196
+00:14:25,570 --> 00:14:29,030
+أزيثروميسين وكلياريثروميسين، هم معانا في الـ
+
+197
+00:14:29,030 --> 00:14:32,050
+presentation اريثروميسين، أزيثروميسين
+
+198
+00:14:32,050 --> 00:14:35,550
+وكلياريثروميسين، مجموعة مهمة بتيجي الصحيح من الـ ..
+
+199
+00:14:35,550 --> 00:14:39,840
+من الـ antibiotics والآن لما نتكلم على مجموعة بتأثر
+
+200
+00:14:39,840 --> 00:14:43,100
+على الـ protein synthesis قسمناهم سابقا إلى 50S
+
+201
+00:14:43,100 --> 00:14:45,840
+Antibiotics و 30S Antibiotics يعني اللي بتأثر على
+
+202
+00:14:45,840 --> 00:14:49,360
+الـ 50S قد يتأثر على الـ 30S حتى اللي بيأثر على الـ
+
+203
+00:14:49,360 --> 00:14:53,180
+50S بيأثره بطريقة وطرق مختلفة ومتنوعة فهنشوف مع
+
+204
+00:14:53,180 --> 00:14:58,760
+بعض إن شاء الله كيف بيشتغل حضرت اللي هو الـ
+
+205
+00:14:58,760 --> 00:15:04,080
+erythromycin أو الـ macro هذه أول خطوة إن في الـ
+
+206
+00:15:04,080 --> 00:15:07,040
+translation في الـ translation أول خطوة إنه يتكون
+
+207
+00:15:07,040 --> 00:15:10,140
+الـ initiation complex المكون منها كان من عناصر
+
+208
+00:15:10,140 --> 00:15:14,660
+واحد mRNA من الـ five to three prime من الـ 30S من
+
+209
+00:15:14,660 --> 00:15:19,180
+هذا الـ الـ الـ amino acyl RNA اللي حامل ميثيونين
+
+210
+00:15:19,180 --> 00:15:22,540
+اللي حامل ميثيونين اللي هو اللي تبع اللي اللي
+
+211
+00:15:22,540 --> 00:15:25,360
+بيحمل الـ anti codon تبع الـ initiation الـ
+
+212
+00:15:25,360 --> 00:15:30,280
+initiation codon صح؟ والـ 50S يبقى في عندي صار هذا
+
+213
+00:15:30,280 --> 00:15:31,480
+بيسموه الـ ribosome complex
+
+214
+00:15:34,240 --> 00:15:41,980
+الخطوة الثانية هي إنه يجيني one new tRNA يعني عكار
+
+215
+00:15:41,980 --> 00:15:46,160
+الـ tRNA ويضرب حمولته في السلسلة فهي تطلع الـ
+
+216
+00:15:46,160 --> 00:15:50,940
+macrolide للأسف ارتباط في المنطقة هاي في الـ 50S
+
+217
+00:15:50,940 --> 00:15:59,190
+هذا الارتباطش بيعمل بيؤدي إلى إن أي new لأ مرتفع،
+
+218
+00:15:59,190 --> 00:16:02,990
+بس اللي جاب له .. اللي جاب له بدي فارغ حمولته
+
+219
+00:16:02,990 --> 00:16:05,570
+من الـ amino acids، هذا بيمنع ما يسمى بالـ
+
+220
+00:16:05,570 --> 00:16:09,810
+Translocation، لانتقال الـ amino acid إلى السلسلة أو
+
+221
+00:16:09,810 --> 00:16:11,710
+السلسلة إلى الـ amino acid جديد
+
+222
+00:16:14,820 --> 00:16:19,160
+أقول بفرغ حمولته وبمشي بدون ما يتكون سلسلة من
+
+223
+00:16:19,160 --> 00:16:24,960
+الـ polypeptide صحيح يعني يبقى معنا الحكي أنا في
+
+224
+00:16:24,960 --> 00:16:28,660
+عندي إنهيبيشن للـ protein synthesis كمحصلة كيف عن
+
+225
+00:16:28,660 --> 00:16:33,500
+طريق إنهيبيشن اللي بتديه الـ transferase اللي هو
+
+226
+00:16:33,500 --> 00:16:37,980
+trans location فبنشوف
+
+227
+00:16:44,670 --> 00:16:52,090
+الثالثة تيتراسايكلين بيشتغل
+
+228
+00:16:52,090 --> 00:16:54,950
+على الـ 50S بيشتغل على 30S مش على 50S
+
+229
+00:16:59,570 --> 00:17:04,030
+كمان مرة translation من البداية لازم نكون عارفين
+
+230
+00:17:04,030 --> 00:17:08,150
+إن التتراسيكلين بيرتبط فقط في وين في الـ 30S وهيك
+
+231
+00:17:08,150 --> 00:17:12,130
+وشايفين هنا شكله وين جال فبعمل bulging، bulging
+
+232
+00:17:12,130 --> 00:17:14,690
+يعني نوع من النتوء في الـ .. في الـ .. في الـ
+
+233
+00:17:14,690 --> 00:17:17,830
+ribosome this prevents the binding of the amino
+
+234
+00:17:17,830 --> 00:17:20,490
+acyl tRNA اللي هو الـ charge .. كلمة charged إيش
+
+235
+00:17:20,490 --> 00:17:25,170
+يعني charged؟ مشحون يعني حامل amino acid to the A
+
+236
+00:17:25,170 --> 00:17:28,920
+-site اللي هو acceptor site إيه المفروض إنه هو
+
+237
+00:17:28,920 --> 00:17:34,080
+الانتيكودون صح؟ ما نعمل ارتباط يعني ما أعرف أحكي إنه
+
+238
+00:17:34,080 --> 00:17:37,700
+it will not accept new amino acids إذا كده كان الـ
+
+239
+00:17:37,700 --> 00:17:42,040
+protein المخلق فيه أربع خمس amino acids هيوقف و
+
+240
+00:17:42,040 --> 00:17:45,700
+انتهى الموضوع ما فيش إن الـ protein احنا هكذا بيطلع
+
+241
+00:17:45,700 --> 00:17:48,920
+عشوائي لازم الـ protein يكون له إيه لازم يكون له
+
+242
+00:17:48,920 --> 00:17:53,360
+تسلسل معين مبني على الـ mRNA اللي موجودة بتاعتي
+
+243
+00:17:53,360 --> 00:17:55,120
+هيكون non-functional
+
+244
+00:17:58,480 --> 00:18:01,700
+طب فيه هنا كامل حاجة تالية let me know if you like
+
+245
+00:18:01,700 --> 00:18:02,120
+both sides
+
+246
+00:18:17,350 --> 00:18:22,750
+الـ Amino Glycosides برضو ترتبط بالـ 30S يبقى كام
+
+247
+00:18:22,750 --> 00:18:26,570
+واحد بالـ 30S بتعرفوا تتراسيكلينز واللي هو الـ
+
+248
+00:18:26,570 --> 00:18:30,050
+Amino Glycosides هاي إثنين 30S تفضلنا ناخد كلمة
+
+249
+00:18:30,050 --> 00:18:35,250
+لإثنين 50S سهلين الـ Chloramphenicol زي
+
+250
+00:18:35,250 --> 00:18:37,710
+الماكرولايدز الماكرولايدز اللي شفناه قبل شوية 
+
+251
+00:18:37,710 --> 00:18:41,330
+اللي يقول عن الارتراوكميسي تبتطلع مع بعض على اللي
+
+252
+00:18:41,330 --> 00:18:45,190
+هو النمل بلاي عشان نشوف كيف ال Amino Glycoside
+
+253
+00:18:45,190 --> 00:18:55,290
+بيشتربنجر طبط وين برضه في ال 30s يبجي
+
+254
+00:18:55,290 --> 00:18:58,730
+عمل earlier termination لمين مش المفروض انه هذا
+
+255
+00:18:58,730 --> 00:19:03,370
+العمل يتوجف متى لما نشوف start كوده فهذا هو كأنه
+
+256
+00:19:03,370 --> 00:19:10,950
+عمل بدل start كوده عمل كأنه start كوده أنا نزلت
+
+257
+00:19:10,950 --> 00:19:14,570
+مدرسة على ال Facebook مبالح المولودين في ملف مضغوط
+
+258
+00:19:19,450 --> 00:19:22,590
+لأ ممكن هو اللي قلته زي ما بعض الأحيان بصير عند
+
+259
+00:19:22,590 --> 00:19:28,230
+بعض الأشكال تفتح الملفات اعمل open with واختاري
+
+260
+00:19:28,230 --> 00:19:33,090
+اللي هو ال internet explorer غالبا بيفتح إن شاء
+
+261
+00:19:33,090 --> 00:19:33,690
+الله معاك الفيديو
+
+262
+00:19:39,050 --> 00:19:42,570
+إذا اتفقنا على إنه المجموعة اللي بتوقف تصنيع ال
+
+263
+00:19:42,570 --> 00:19:45,830
+protein synthesis في منها بيشتغل على ال 50S و بيشتغل 
+
+264
+00:19:45,830 --> 00:19:50,390
+على ال 30S ناخد أشهرهم ال chloramphenicol اللي كان في
+
+265
+00:19:50,390 --> 00:19:54,010
+يوم من الأيام كان في يوم من الأيام drug of choice
+
+266
+00:19:54,010 --> 00:19:56,750
+لعلاج كثير من الأمراض منها التيفويت فيها
+
+267
+00:20:00,980 --> 00:20:05,640
+باستمرار وبنستفيد من المعلومات تبعتنا لاجينا إنه
+
+268
+00:20:05,640 --> 00:20:09,760
+الناس اللي بياخدوا ال chloramphenicol لفترة طويلة
+
+269
+00:20:09,760 --> 00:20:12,400
+بيصير عندهم bone marrow depression وممكن يصير
+
+270
+00:20:12,400 --> 00:20:15,300
+عندهم نوع من ال anemia اسمه aplastic anemia
+
+271
+00:20:15,300 --> 00:20:20,340
+وبالتالي ال chloramphenicol تم إيقاع استخدامه
+
+272
+00:20:20,340 --> 00:20:24,760
+systematically يعني oral أو injection و
+
+273
+00:20:24,760 --> 00:20:29,460
+فقط مقصود استخدامه الآن على typical applications
+
+274
+00:20:29,460 --> 00:20:36,080
+مثل ما شاهدنا قبل برود سبكترام وسط تايف .. نستخدمه
+
+275
+00:20:36,080 --> 00:20:39,380
+بعض الحين في المقتبع اللي بنضيفه على ال media لقتل
+
+276
+00:20:39,380 --> 00:20:41,840
+البكتيريا لأنه برود سبكترام فاللي ابقى بدأ ألمي
+
+277
+00:20:44,500 --> 00:20:47,320
+كلورامفينيكولى على الوسط أسابقته المين البكتيريا
+
+278
+00:20:47,320 --> 00:20:51,800
+عشان نسمع لها الفيطريات تانية it binds to the 50S
+
+279
+00:20:51,800 --> 00:20:54,980
+ribosomal subunit and inhibits peptide bond formation
+
+280
+00:20:54,980 --> 00:20:58,760
+of the protein synthesis الأمينoglycosides
+
+281
+00:20:58,760 --> 00:21:02,080
+أخذنا أمثلة أو عليها أمثلة streptomycin نيوميسين
+
+282
+00:21:02,080 --> 00:21:06,120
+and جنتاميسين خليني أحكي شوية عن المجموعة دي لإن
+
+283
+00:21:06,120 --> 00:21:08,500
+هي مجموعة تتغير من المجموعات المهمة برضه في ال
+
+284
+00:21:08,500 --> 00:21:14,920
+antibiotics هذه المجموعة تتميز بسميتها العالية اللي
+
+285
+00:21:14,920 --> 00:21:18,460
+هي سمية على الإنسان و بيسموها the three toxicities
+
+286
+00:21:18,460 --> 00:21:24,200
+السميات oto, nephro and neuro oto اللي هي العصب
+
+287
+00:21:24,200 --> 00:21:30,460
+السمعي و nephro اللي هي الكلى و ال neuro ال
+
+288
+00:21:30,460 --> 00:21:35,410
+toxicity اللي هي العصب بشكل عام هذه أنا بتقولها من
+
+289
+00:21:35,410 --> 00:21:39,710
+نموذج نموذج ليش احنا في التحاليل الطبية أو في
+
+290
+00:21:39,710 --> 00:21:43,390
+الصيدلة القصر ما نوصفش للناس قد يحط لو احنا اللي
+
+291
+00:21:43,390 --> 00:21:47,640
+عزلنا ال microbe ع مدار ال sensitivity testing احنا
+
+292
+00:21:47,640 --> 00:21:50,960
+اللي بنعرف عن المريض حاجة واحدة العينة اللي
+
+293
+00:21:50,960 --> 00:21:54,700
+أخدناها منه والنتيجة تبعتنا صح هذا لأ لكن الطبيب
+
+294
+00:21:54,700 --> 00:21:58,100
+بيعرف عن المريض معلومات أكثر بيعرف symptoms و
+
+295
+00:21:58,100 --> 00:22:01,700
+بيعرف history لو كان المريض هذا عنده kidney
+
+296
+00:22:01,700 --> 00:22:04,480
+failure أو بداية kidney failure أو قصور في الكلى
+
+297
+00:22:04,480 --> 00:22:08,760
+الجرعة اللي بتعطيها من ال aminoglycosides يجب أن
+
+298
+00:22:08,760 --> 00:22:14,440
+تكون جرعة أصغر لأن ال elimination لهذا ال drug
+
+299
+00:22:14,440 --> 00:22:19,040
+بيكون عبر الكلى طب إذا الكلية أصلا فيها قصور فال
+
+300
+00:22:19,040 --> 00:22:21,440
+elimination مش هيكون طبيعي وبالتالي هيزيد عمره
+
+301
+00:22:21,440 --> 00:22:25,180
+النصف في الدم، إذا زاد عمره في الدم و أصلا بيعمل
+
+302
+00:22:25,180 --> 00:22:28,400
+nephrotoxicity يبقى هيزيده من إحدة لإحدى للكلية
+
+303
+00:22:28,400 --> 00:22:31,120
+بزيادة، هو عنده أصلا مشكلة و احنا بنضيف له على
+
+304
+00:22:31,120 --> 00:22:35,440
+مشكلته ضعف جديدة، بندمره بقالين في الكلية، لذلك
+
+305
+00:22:35,440 --> 00:22:39,440
+إياكم في يوم من الأيام هتصرف ما يمكن توصفه
+
+306
+00:22:39,440 --> 00:22:42,860
+Antibiotics لإنه يبقى لكوا في بعض يعني الصورة غير
+
+307
+00:22:42,860 --> 00:22:45,780
+مكتملة زي الكلام اللي احنا حكيناه عجب شوية لما
+
+308
+00:22:45,780 --> 00:22:48,640
+الواحد بيرحل على معلومة و بيستغلها بيستعجل في
+
+309
+00:22:48,640 --> 00:22:54,260
+استغلالها قد تؤذي الاستعجال أو لما تكون مبنية أو
+
+310
+00:22:54,260 --> 00:22:57,940
+القرار مبني على معلومات قليلة للأسف يكون القرار مش
+
+311
+00:22:57,940 --> 00:23:00,900
+جيد لكن لما تكون معلومة مبنية على صورة كاملة لما
+
+312
+00:23:00,900 --> 00:23:01,540
+تكون القرار
+
+313
+00:23:08,340 --> 00:23:15,080
+لوصف علاجات استراتومايسين نيومايسين and جنتومايسين
+
+314
+00:23:15,080 --> 00:23:19,000
+هذه المجموعة .. أفضل أنا قلت 3 منهم لكن هم يمكن
+
+315
+00:23:19,000 --> 00:23:23,220
+أكثر من 20 أكثر من 20 كلهم بتميزوا بسميات .. لكن
+
+316
+00:23:23,220 --> 00:23:28,040
+سمياتهم مختلفة يعني مثلا الجنتومايسين مستخدم لحد
+
+317
+00:23:28,040 --> 00:23:34,720
+اليوم بشكل كبير الكنامايسين الاميكاسين أسمع بي
+
+318
+00:23:34,720 --> 00:23:37,780
+ممنوع على الصبورة، لكن much more safer than
+
+319
+00:23:37,780 --> 00:23:41,500
+neomycin، neomycin is not given systematically
+
+320
+00:23:41,500 --> 00:23:46,320
+بالمرة، ممنوع يُعطى، فلازم مرنجلانين لل typical،
+
+321
+00:23:46,320 --> 00:23:49,500
+ده كام واحد typical أخدته اليوم، مين حافظهم؟
+
+322
+00:23:54,150 --> 00:23:58,630
+وهي نيوميسين و هناخد واحد كمان شوية اللي هو
+
+323
+00:23:58,630 --> 00:24:03,650
+بوليمكسين بيه بتاع ال cell membrane وبالتالي بيكون
+
+324
+00:24:03,650 --> 00:24:07,490
+هيك ألفنا مجموعة من ال antibiotic استخدامها فقط
+
+325
+00:24:07,490 --> 00:24:11,050
+مقصور على مين على ال typical application يبدو أنه
+
+326
+00:24:11,050 --> 00:24:15,650
+اليوم إن شاء الله هنكمل مع بعض روض لي مجموعة من
+
+327
+00:24:15,650 --> 00:24:20,330
+الطالبات و نبدأ مع طالبة همسة تعفش بموضوع
+
+328
+00:24:26,060 --> 00:24:29,680
+السلام عليكم اليوم بحكي لكم عن بكتيريا اسمها
+
+329
+00:24:29,680 --> 00:24:32,940
+cyanobacteria هاي ال cyanobacteria بتتميز لأن
+
+330
+00:24:32,940 --> 00:24:35,440
+عندها اللونين هما ال green و ال blue السبب
+
+331
+00:24:35,440 --> 00:24:38,940
+باكتشافها إن كانوا زمان أول ما وجدوها حكوا عنها
+
+332
+00:24:38,940 --> 00:24:41,880
+أنها تحالب ليش حكوا عنها تحالب بسبب وجود هدولة
+
+333
+00:24:41,880 --> 00:24:47,560
+اللونين اليوم بدأوا يحكوا كمان عن أن هي بكتيريا قديمة
+
+334
+00:24:47,560 --> 00:24:54,510
+جديدة موجودة حيث قالت بعد البحثات أن الكركب موجودة من
+
+335
+00:24:54,510 --> 00:24:58,270
+اثنين ونصف مليار سنة 2.5 billion years إذا أن هي موجودة من
+
+336
+00:24:58,270 --> 00:25:02,730
+آلاف السنين من الملايين حتى كمان السنين هي طبعا
+
+337
+00:25:02,730 --> 00:25:06,310
+موجودة بتقدر تعيش في الميه بتقدر تعيش في السولات
+
+338
+00:25:06,310 --> 00:25:09,610
+بتقدر تعيش حاليا ال skin smear وموجودة كمان في بعض
+
+339
+00:25:09,610 --> 00:25:12,850
+الخضروات موجودة كمان في ال soil كمان أن هي
+
+340
+00:25:12,850 --> 00:25:15,770
+موجودة
+
+341
+00:25:17,480 --> 00:25:21,080
+أول ما بدأوا أنهم يكتشفوها .. اكتشفوها في دول
+
+342
+00:25:21,080 --> 00:25:25,980
+إسكندنافية خاصة دولة فنلاندا، دولة فنلاندا كانوا
+
+343
+00:25:25,980 --> 00:25:30,260
+لما اكتشفوها لاحظوا أن في ارتفاع درجات الحرارة في
+
+344
+00:25:30,260 --> 00:25:32,780
+الميه، لما اكتشفوا أن في ارتفاع درجات الحرارة في
+
+345
+00:25:32,780 --> 00:25:36,320
+الميه فقدروا أن موجود هذه البكتيريا وكمان ارتفاع
+
+346
+00:25:36,320 --> 00:25:40,880
+درجات الحرارة في الميه بتساعدها على نموها بشكل
+
+347
+00:25:40,880 --> 00:25:46,880
+كبير حيث  انتحار .. و هلأ في عندها ثلاث أنواع أول
+
+348
+00:25:46,880 --> 00:25:52,040
+نوع اللي هو نوع أخضر وجود النوع الأخضر بسبب وجود
+
+349
+00:25:52,040 --> 00:25:54,760
+mad chlorophyll اللي بيساعدها في عملية البناء
+
+350
+00:25:54,760 --> 00:25:58,620
+الضوئي و أيضا في عندنا كمان لون أحمر اللون الأحمر
+
+351
+00:25:58,620 --> 00:26:02,460
+اكتشفوه في طائر اسمه ال flamingo هذا الطائر ال
+
+352
+00:26:02,460 --> 00:26:05,440
+flamingo لما كان بده يشرب .. لما بيشرب من هذه
+
+353
+00:26:05,440 --> 00:26:11,480
+الميه بيصير لون جسمه سبانك فعشان هيك وجود لون أحمر
+
+354
+00:26:11,480 --> 00:26:18,530
+أو بكتيريا حمراء أما اللون الأزرق فهو طبعا سبب بعض
+
+355
+00:26:18,530 --> 00:26:21,490
+الصبغات، هنا أنا بدأ أحكيلكوا أنا عن بعض صفاتها
+
+356
+00:26:21,490 --> 00:26:24,630
+اللي هو عبارة عن ground water tap و هي طبعا
+
+357
+00:26:24,630 --> 00:26:29,310
+obligate و هي motility تحت ال microscope و هي
+
+358
+00:26:29,310 --> 00:26:32,830
+حجمها كثير صغير unicellular و هي مافيهاش
+
+359
+00:26:35,430 --> 00:26:40,130
+هنا بس بقيت أحكيلكوا قبل ما نحكي عن ال .. الميادات
+
+360
+00:26:40,130 --> 00:26:44,970
+تبعتها بقيت أحكي عن بعض المساورت تبعتها عشان
+
+361
+00:26:44,970 --> 00:26:49,330
+الميه، لما احنا حكينا إن كانت موجودة في الميه
+
+362
+00:26:49,330 --> 00:26:54,830
+بتعمل ارتفاع درجات الحرارة، أي إنسان لما بت .. بعد
+
+363
+00:26:54,830 --> 00:26:57,770
+المساورة كانت تظهر على المنازلين هناك، أي واحد روح
+
+364
+00:26:57,770 --> 00:27:03,610
+يسبح، كانت بتصيب في عنده حساسية على الجلد أو بعض
+
+365
+00:27:03,610 --> 00:27:07,770
+الالتهابات في العين وكمان هذه البكتيريا كانت بتغذى
+
+366
+00:27:07,770 --> 00:27:11,990
+على مادة الفسفور الموجودة في النباتات، في التحالب،
+
+367
+00:27:11,990 --> 00:27:18,610
+طحالب ميتة أو بعض الأسماك هناك لما الإنسان كمان
+
+368
+00:27:18,610 --> 00:27:22,650
+ممكن بده ياكل مأكولات بحرية خاصة من دولة فنلاندا
+
+369
+00:27:22,650 --> 00:27:26,730
+كان بيصير عنده إسهال وكانت بيصير في عنده صداع
+
+370
+00:27:26,730 --> 00:27:30,290
+شديد و أيضا درجات الحرارة، بترتفع عنده درجة الحرارة
+
+371
+00:27:31,520 --> 00:27:36,020
+رغم أن .. أن هي طبعا مضرة إلا أنها مفيدة جدا
+
+372
+00:27:36,020 --> 00:27:40,900
+للبيئة، أول فائدة أن هي للبيئة أن هي كانت بتقدر
+
+373
+00:27:40,900 --> 00:27:44,800
+تستطيع تحليل مادة السيليلوز الموجودة في النباتات أو
+
+374
+00:27:44,800 --> 00:27:48,540
+الخشب أن هي لما تحلل مادة السيليلوز في النباتات
+
+375
+00:27:48,540 --> 00:27:55,010
+بيساعدها على تشكيل بتساعد النباتات بتشكل هيكل 
+
+376
+00:27:55,010 --> 00:28:01,270
+النبات بيعطيها المرونة بيعطيها الصلابة وفي برضه
+
+377
+00:28:01,270 --> 00:28:07,170
+باحث بيحكي إنه تلتين من هذه البكتيريا بتعمل على
+
+378
+00:28:07,170 --> 00:28:11,230
+تثبيت النيتروجين هو nitrogen fixation هنا عندنا
+
+379
+00:28:11,230 --> 00:28:15,730
+رسمة مختصرة عن تثبيت النيتروجين إحنا عارفين إن
+
+380
+00:28:15,730 --> 00:28:19,600
+النيتروجين موجود في الجو كتير من التربة مافيهاش
+
+381
+00:28:19,600 --> 00:28:22,220
+خالي هو من الـ nitrogen فهذا عندي أنا النوع من
+
+382
+00:28:22,220 --> 00:28:29,020
+البكتيريا إنه بيساعد البكتيريا الموجودة
+
+383
+00:28:29,020 --> 00:28:31,840
+في التربة بتساعد بعض البكتيريا على إنها تستطيع
+
+384
+00:28:31,840 --> 00:28:35,920
+تثبيت الـ nitrogen عندنا هون بكتيريا تثبيت الـ
+
+385
+00:28:35,920 --> 00:28:41,010
+nitrogen أول حاجة بتاخد الـ nitrogen من الجو هذه
+
+386
+00:28:41,010 --> 00:28:44,530
+البكتيريا بتقوم بتثبيته، بتحوله إلى أمونيوم، بعد
+
+387
+00:28:44,530 --> 00:28:48,690
+هيك بتكون عندها بكتيريا اسمها بكتيريا نترجة، بتحوله
+
+388
+00:28:48,690 --> 00:28:54,790
+لـ NO2، بعد هيك بتحوله لناترات، ناترات هذا كمان في
+
+389
+00:28:54,790 --> 00:28:58,570
+عندها بكتيريا محللة للنيتروجين من الناترات، بتوصله
+
+390
+00:28:58,570 --> 00:29:02,830
+للجو، وأيضا كمان في عندنا نباتات بتستطيع تحطم هذا
+
+391
+00:29:02,830 --> 00:29:06,930
+الناترات، وهيك بيكون خلصت أنا الموضوع لليوم هذا
+
+392
+00:29:08,520 --> 00:29:12,680
+الشارع هنفصل عنه وأنا الطالبة همسة طافش شكرا لكم
+
+393
+00:29:15,290 --> 00:29:18,470
+السلام عليكم، اليوم موضوعنا عن الـ Helicobacter pylori هي
+
+394
+00:29:18,470 --> 00:29:23,130
+المتواجد من المعدة البكتيرية، هذه معناها اللي من
+
+395
+00:29:23,130 --> 00:29:27,810
+نوع الـ helobacter، helo معناها حلزوني، bacter،
+
+396
+00:29:27,810 --> 00:29:31,510
+بكتيريا، بالوري معناها اللي هي بتوجد في البوابة ما
+
+397
+00:29:31,510 --> 00:29:36,710
+بين المعدة والاثني عشر تم اكتشافها قديمًا في 1875
+
+398
+00:29:36,710 --> 00:29:41,730
+على اعتماد إن هي بكتيريا تعيش في المعدة بسبب وجود
+
+399
+00:29:41,730 --> 00:29:47,600
+إنزيمات وأحماض في المعدة، ولكن بعد ذلك في 1879 تم
+
+400
+00:29:47,600 --> 00:29:52,480
+عزلها فعلًا في المختبر وفي الـ ... وفي 2005 حصلوا
+
+401
+00:29:52,480 --> 00:29:57,100
+على جائزة نوبل لعزل هذه البكتيريا هذه البكتيريا
+
+402
+00:29:57,100 --> 00:30:01,840
+بتعيش في المعدة لعدة أسباب بتمكنها أو لظروف مناسبة
+
+403
+00:30:01,840 --> 00:30:08,140
+لها منها إنه في عندها إنزيمات بتعيش في الغشاء
+
+404
+00:30:08,140 --> 00:30:13,280
+المبطن اللي بيغلف المعدة، فبتكون مختفية نوعًا ما بها
+
+405
+00:30:13,280 --> 00:30:17,340
+السبب الثاني إن عندها بتفرز إنزيم الـ urease هذا
+
+406
+00:30:17,340 --> 00:30:21,060
+بيعمل على تحويل إفرازات المعدة إلى أمونيا الأمونيا
+
+407
+00:30:21,060 --> 00:30:24,560
+طبعًا قلوية فبتعادل الحمض المعدة بالتالي بتساعدها
+
+408
+00:30:24,560 --> 00:30:28,740
+العيش الثاني السبب الثالث إن ممكن الأغذية اللي
+
+409
+00:30:28,740 --> 00:30:32,400
+بتكون تحتوي على نسبة كبيرة من السكريات والأملاح
+
+410
+00:30:32,400 --> 00:30:37,540
+والدهون بتساعد تكثرها بشتر طرق انتقالها
+
+411
+00:30:40,830 --> 00:30:44,010
+طرق انتقال طبعًا عن الـ contamination الـ food والـ
+
+412
+00:30:44,010 --> 00:30:50,730
+water وممكن كمان من person to person في نوع منها
+
+413
+00:30:50,730 --> 00:30:54,150
+بينتقل عن طريق الـ حيوانات الأليفة والكلاب طبعًا هاي نص
+
+414
+00:30:54,150 --> 00:30:59,930
+سكان العالم بيصابوا بهذه الـ bacteria الـ bacteria
+
+415
+00:30:59,930 --> 00:31:05,870
+هذه لها كذا أعراض من هذه الأعراض اللي ممكن تكون غثيان
+
+416
+00:31:05,870 --> 00:31:11,830
+يعني، ممكن تقيؤ، فقدان بالوزن، ممكن شعور بالجوع
+
+417
+00:31:11,830 --> 00:31:16,790
+بعد الأكل، ولكن هذه الأعراض مش ... مش كل الناس نفس
+
+418
+00:31:16,790 --> 00:31:20,170
+الأعراض، ممكن تكون خفيفة، ممكن تكون هذه الأعراض
+
+419
+00:31:20,170 --> 00:31:25,130
+بالضبط متواجدة عندهم، ووجود إن حوالي 85% من
+
+420
+00:31:25,130 --> 00:31:29,050
+الأشخاص المصابين بقرحة المعدة بتكون
+
+421
+00:31:29,050 --> 00:31:34,120
+عندهم متواجدة البكتيريا الحلزونية، وفي الـ 20 الـ
+
+422
+00:31:34,120 --> 00:31:39,340
+30 سنة عندهم بتكون معرضين للإصابة بقرحة المعدة
+
+423
+00:31:39,340 --> 00:31:44,940
+بهذه البكتيريا، وفي أيضا من القرح الشائعة اللي
+
+424
+00:31:44,940 --> 00:31:51,020
+هي مرض شلل الرعاش، وجدوا إن هذه البكتيريا لها سبب
+
+425
+00:31:51,020 --> 00:31:54,820
+رئيسي في هذا المرض، لإنه لو ييجوا يحللوه ممكن تكون
+
+426
+00:31:54,820 --> 00:31:58,580
+عندهم positive result في هذه البكتيريا لذلك يجب أن
+
+427
+00:31:58,580 --> 00:32:05,110
+نعالجها حتى تختفي هذا المرض شلل الرعاش، كيف بتقدر
+
+428
+00:32:05,110 --> 00:32:08,670
+البكتيريا تسبب شلل الرعاش؟ إن البكتيريا بتفرز مادة شبيه
+
+429
+00:32:08,670 --> 00:32:13,990
+بالكولسترول تسمى modified cholesterol هذه مادة سامة
+
+430
+00:32:13,990 --> 00:32:17,090
+للجهاز العصبي وبتسبب هذا المرض
+
+431
+00:32:19,930 --> 00:32:24,610
+كمان إلها دور مهم في موضوع الأنيميا بتعمل على نقص
+
+432
+00:32:24,610 --> 00:32:29,310
+امتصاص الحديد في الجسم لإن هي أصلا تحتاج الحديد في
+
+433
+00:32:29,310 --> 00:32:33,930
+تكاثرها، كمان في أنواع من الأنيميا الخفيفة اسمها
+
+434
+00:32:33,930 --> 00:32:37,950
+pernicious anemia هاي بتسبب لها autoimmune
+
+435
+00:32:37,950 --> 00:32:42,490
+gastritis بتعمل التهاب في المعدة بتعمل على منع
+
+436
+00:32:42,490 --> 00:32:47,510
+امتصاص الـ vitamin B12 كيف هنشخص هاي البكتيريا؟
+
+437
+00:32:48,520 --> 00:32:56,300
+أولًا عن طريق اللي هو blood test فيننا blood
+
+438
+00:32:56,300 --> 00:33:01,620
+test blood test هنبحث عن الـ antibodies للعينة إذا
+
+439
+00:33:01,620 --> 00:33:06,040
+كان موجود في عينة الـ IgM إذا هتكون positive لهذه
+
+440
+00:33:06,040 --> 00:33:10,460
+البكتيريا الـ IgG هنستخدمها لو كان في عينة كتير ممكن
+
+441
+00:33:10,460 --> 00:33:16,000
+نستخدم اللي بتبين في عينة اللي هي IgG، ولكن إنه هنا إن
+
+442
+00:33:16,000 --> 00:33:19,860
+الـ antibody بتبقى شهور وسنين موجودة في العينة
+
+443
+00:33:19,860 --> 00:33:23,940
+ممكن تعطي positive result حتى بعد العلاج، ولكن ممكن
+
+444
+00:33:23,940 --> 00:33:28,900
+يستخدم أو أفضل استخدام التحليل اللي هو stool test
+
+445
+00:33:28,900 --> 00:33:33,660
+stool test ممكن يعطي اللي هو false negative حتى
+
+446
+00:33:33,660 --> 00:33:39,470
+بعد ما ينتهي العلاج، في كمان عن طريق اللي هو المنظار
+
+447
+00:33:39,470 --> 00:33:44,010
+هناخد عينة من جزء المصاب من المعدة وبنحللها
+
+448
+00:33:44,010 --> 00:33:47,790
+لنشوف ... نكتشف مادة اليورياز، إذا اليورياز موجود
+
+449
+00:33:47,790 --> 00:33:54,110
+إذا البكتيريا موجودة وبتكون ناشطة، في هنا الـ breath
+
+450
+00:33:54,110 --> 00:33:59,870
+test هذا عبارة إنه إن الإنسان المريض بينفخ في كيس
+
+451
+00:34:00,250 --> 00:34:03,370
+هناخد العينة اللي نفخ فيها بالكيس هنحطها على
+
+452
+00:34:03,370 --> 00:34:06,910
+الكربونا والكربونا هنحطها على الجهاز فهنقيس
+
+453
+00:34:06,910 --> 00:34:11,670
+ونقرأها نقرأ اللي هل موجودة عنده مثلا البكتيريا أو
+
+454
+00:34:11,670 --> 00:34:17,070
+لا هنا هذه ممتازة جداً ولكن مكلفة جداً جداً هادي طيقة
+
+455
+00:34:17,070 --> 00:34:20,570
+ويُفضل قاعدة الفحص عند أخذ العلاج من خلال هو أربع
+
+456
+00:34:20,570 --> 00:34:25,720
+لست أسابيع، طرق العلاج فيه هنا إن بالرغم من كل
+
+457
+00:34:25,720 --> 00:34:29,340
+سلبيات هذه البكتيريا ولكن إن البعض بيفضل إنه
+
+458
+00:34:29,340 --> 00:34:33,400
+مانعالجهاش إذا ماكانش فيه هنا مشاكل لإن هي بتسبب
+
+459
+00:34:33,400 --> 00:34:38,710
+في علاج الالتهابات أو القولون، تقليل من تهيجات
+
+460
+00:34:38,710 --> 00:34:43,310
+القولون، ولكن لو إحنا بدنا نيجي نعالجها حاول الكثير
+
+461
+00:34:43,310 --> 00:34:47,170
+من العلماء اكتشاف vaccine ضد هذه البكتيريا، ولكن هم
+
+462
+00:34:47,170 --> 00:34:50,870
+يستطيعوا بسبب وجود أنواع كثيرة من هذه البكتيريا
+
+463
+00:34:50,870 --> 00:34:56,030
+فهنا في الآخر إن اكتشفوا triple therapy مناد حيوي
+
+464
+00:34:56,030 --> 00:35:01,670
+عبارة بيحتوي على الـ ... الـ omeprazole وفي عندنا
+
+465
+00:35:01,670 --> 00:35:04,830
+الـ amoxicillin هدول هيكون الـ antitherapy ضد
+
+466
+00:35:04,830 --> 00:35:09,900
+البكتيريا، السلام عليكم ورحمة الله وبركاته أنا
+
+467
+00:35:09,900 --> 00:35:13,300
+الطالبة هاي بخلص بدي أحكي عن موضوع الـ probiotic
+
+468
+00:35:13,300 --> 00:35:20,480
+بشكل مختصر what are probiotic? probiotic are live
+
+469
+00:35:20,480 --> 00:35:24,380
+microorganism in most cases bacteria and yeast
+
+470
+00:35:24,380 --> 00:35:27,880
+that are similar to beneficial microorganism found
+
+471
+00:35:27,880 --> 00:35:34,820
+in human gut إذا probiotic هي عبارة عن كائنات حية
+
+472
+00:35:34,820 --> 00:35:42,910
+دقيقة، أغلب الأحيان بكتيريا وخمائر موجودة في أمعاء
+
+473
+00:35:42,910 --> 00:35:45,590
+الإنسان بشكل طبيعي، تقريبًا زي الـ normal في القرح
+
+474
+00:35:45,590 --> 00:35:50,530
+نقدر نسميها اسم ثاني اللي هي الـ friendly bacteria
+
+475
+00:35:50,530 --> 00:35:54,650
+والـ good bacteria يعني ما بتسببنا pathogen أمراض
+
+476
+00:35:54,650 --> 00:35:59,310
+وموجودة في الـ dietary supplement اللي هي
+
+477
+00:35:59,310 --> 00:36:02,370
+المكملات الغذائية والـ food في العديد من الأوقات
+
+478
+00:36:02,370 --> 00:36:09,770
+عامة بنلاقيه، how do probiotic work؟ كيف بيشتغل الـ
+
+479
+00:36:09,770 --> 00:36:15,910
+probiotic في الجسم؟ probiotic
+
+480
+00:36:15,910 --> 00:36:20,010
+can improve intestinal function and maintain the
+
+481
+00:36:20,010 --> 00:36:24,330
+integrity of the lining of the intestine اللي هو
+
+482
+00:36:24,330 --> 00:36:31,720
+بيساعد ... بيحمل بيحمل بطانة الداخلية للأمعاء وكمان
+
+483
+00:36:31,720 --> 00:36:37,040
+بيسهل حركة الطعام أثناء الأمعاء بحيث إنه بيمنع إنه
+
+484
+00:36:37,040 --> 00:36:40,280
+يمشي بشكل سريع يسبب إسهال أو بشكل بطيء يسبب
+
+485
+00:36:40,280 --> 00:36:44,500
+constipation Probiotic
+
+486
+00:36:44,500 --> 00:36:47,780
+may help lots of ailments يعني ممكن تحل العديد من
+
+487
+00:36:47,780 --> 00:36:52,400
+الأمراض عندنا treating childhood diarrhea treating
+
+488
+00:36:52,400 --> 00:36:58,260
+ulcerative colitis اللي هي الـ ... قرح القولون
+
+489
+00:36:58,260 --> 00:37:04,380
+treating necrotizing enterocolitis هذه الالتهابات
+
+490
+00:37:04,380 --> 00:37:07,820
+بتنتج عند الأطفال حديثي الولادة ممكن تسبب للوفاة
+
+491
+00:37:07,820 --> 00:37:13,160
+prevent antibiotic associated diarrhea and infectious
+
+492
+00:37:13,160 --> 00:37:20,760
+إسهال في بعض الـ antibiotic ممكن ينتج عنها إسهال
+
+493
+00:37:20,760 --> 00:37:25,560
+الـ property بتمنع هذا الأشياء زي الـ clindamycin
+
+494
+00:37:26,550 --> 00:37:30,090
+Preventing Pouchitis إنها الالتهابات اللي بتنتج عن
+
+495
+00:37:30,090 --> 00:37:34,450
+عمليات جراحية للأمعاء Treating and Preventing
+
+496
+00:37:34,450 --> 00:37:39,130
+Eczema أساسات الأكزيما، هي حالة حساسية بتنتج عن
+
+497
+00:37:39,130 --> 00:37:43,810
+بعض اللي عن شرب حليب البقر، البروبايوتك بتمنعها
+
+498
+00:37:43,810 --> 00:37:50,470
+Helping the immune system طبعًا هنا كيف البروبا
+
+499
+00:37:50,470 --> 00:37:55,650
+يوتك بتعمل أهمية إنها help in gut health يعني حفاظ
+
+500
+00:37:55,650 --> 00:38:02,050
+على الأمعاء، هذا مثال lactobacillus مثال على نوع من 
+
+501
+00:38:02,050 --> 00:38:05,650
+أنواع البكتيريا التي بروبايوتك موجودة في ال
+
+502
+00:38:05,650 --> 00:38:10,730
+digestive system والـurinary والـgenital system طبعا
+
+503
+00:38:10,730 --> 00:38:14,690
+بشكل طبيعي ما بتسبب أمراض، إنه في العديد من ال
+
+504
+00:38:14,690 --> 00:38:17,870
+species، كثير منها أهمها الـlactobacillus 
+
+505
+00:38:17,870 --> 00:38:23,430
+acidophilus، أشهر الأنواع التي most commonly used in
+
+506
+00:38:23,430 --> 00:38:26,470
+probiotic، والتي friendly bacteria
+
+507
+00:38:28,850 --> 00:38:33,130
+طبعا بما أنه بس .. بس يعني عصوية هي لك تبصيه بس
+
+508
+00:38:33,130 --> 00:38:40,050
+كنت بشربها على شكل، قدر المصادر الممكن نحصل من خلالها
+
+509
+00:38:40,050 --> 00:38:45,050
+على الـprobiotic بنلاقيها في الـyogurt، اللي هو
+
+510
+00:38:45,050 --> 00:38:51,270
+الزبادي، الحليب، اللبن يعني، الحليب، الياغورت، 
+
+511
+00:38:51,270 --> 00:38:55,110
+التوفو، القمح، الكفير، ال .. الـhoney
+
+512
+00:38:59,530 --> 00:39:03,410
+available form، كيف ممكن نبيعها لل .. للـ
+
+513
+00:39:03,410 --> 00:39:06,510
+probiotic؟ ممكن تكون على شكل frozen، جان ..
+
+514
+00:39:06,510 --> 00:39:10,290
+granular، على حبيبات أو powder، capsule ممكن تكون 
+
+515
+00:39:10,290 --> 00:39:15,530
+سائلة، liquid أو ممكن تكون على شكل tablets، أقراص
+
+516
+00:39:15,530 --> 00:39:16,830
+أو للتحرير
+
+517
+00:39:21,410 --> 00:39:25,110
+بسم الله الرحمن الرحيم، السلام عليكم، الموضوع اليوم
+
+518
+00:39:25,110 --> 00:39:29,450
+بيربط ما بين الـE.coli وما بين الملاريا، هلأ كيف
+
+519
+00:39:29,450 --> 00:39:32,130
+..عارفين بتعرفوا، بالنسبة للي راح نأخذ نفس صغيرة
+
+520
+00:39:32,130 --> 00:39:35,450
+عن مرض الملاريا، راح نأخذ نفس صغيرة عن مرض
+
+521
+00:39:35,450 --> 00:39:39,110
+الملاريا، بالنسبة لمرض الملاريا هو من الأمراض .. هو
+
+522
+00:39:39,110 --> 00:39:43,680
+مرض بسبب طفيلي اسمه الـplasmodium، هذا المرض بيكون
+
+523
+00:39:43,680 --> 00:39:47,160
+الـvector له، أو بينتقل من إنسان لإنسان عن
+
+524
+00:39:47,160 --> 00:39:53,200
+طريق بعوضة الـanopheles، شائع أو منتشر هذا المرض في
+
+525
+00:39:53,200 --> 00:39:56,100
+مناطق .. في مناطق هطول الأمطار، المناطق التي بيكتب
+
+526
+00:39:56,100 --> 00:40:00,180
+فيها المستنقعات، وخاصة الدول الأفريقية، الأفريقية،
+
+527
+00:40:00,180 --> 00:40:07,920
+في عام 2012 سجلت حوالي 627 ألف حالة وفاة نتيجة
+
+528
+00:40:07,920 --> 00:40:12,160
+للإصابة بالملاريا، 90% منهم كان من الأطفال، ركزوا
+
+529
+00:40:12,160 --> 00:40:14,940
+على هذه النقطة التي احنا بنعلمه، حلو بالنسبة لي
+
+530
+00:40:14,940 --> 00:40:19,000
+دورة ال .. دورة ال .. دورة الـplasmodium، أو دورة
+
+531
+00:40:19,000 --> 00:40:22,840
+.. دورة دورة المرض مش كتير، راح نتعمق فيها، لكن
+
+532
+00:40:22,840 --> 00:40:25,540
+فيه هنا بعوضة، هذه البعوضة بتحتوي على الـ
+
+533
+00:40:25,540 --> 00:40:29,920
+plasmodium، ب .. فيه قلوب دورته two cycle، دورة
+
+534
+00:40:29,920 --> 00:40:33,760
+بيحصل في البعوضة، ودورة في الإنسان، حلو، بيقعد في
+
+535
+00:40:33,760 --> 00:40:37,800
+البعوضة حوالي أسبوع، بعدين بينتقل يصل لعند ال ..
+
+536
+00:40:37,800 --> 00:40:41,400
+الغدد اللعابية لإلها، لما تعمل بيت لإنسان سليم
+
+537
+00:40:41,730 --> 00:40:46,470
+بدها تحقنه في الـparasite، هلأ وصل الـparasite ال
+
+538
+00:40:46,470 --> 00:40:51,270
+target الأول لإله، رح يكون في الـliver، طبعا في ال
+
+539
+00:40:51,270 --> 00:40:55,250
+liver بيضلوا حوالي أسبوع على ما هو بيستهدف الـRBCs
+
+540
+00:40:55,250 --> 00:40:59,990
+بيضلوا فيها حوالي من يومين ل 3 أيام، بعدين بسبب
+
+541
+00:40:59,990 --> 00:41:03,990
+لإلها lysis بتطلع عندك الميه من الـparasite
+
+542
+00:41:03,990 --> 00:41:09,150
+وبالتالي بتنتشر في الجسم، طبعا بتكون systemic لإن
+
+543
+00:41:09,150 --> 00:41:13,270
+الدم موجود وين ما كان، بعدين لو إجت .. لو إجت بعض
+
+544
+00:41:13,270 --> 00:41:17,990
+تانية وراحت .. أخدت من ذنب هذا المصاب طبعا، و
+
+545
+00:41:17,990 --> 00:41:25,230
+أحققته أو عملت بثلة، إن إنسان سليم أخدت انتقل لشخص
+
+546
+00:41:25,230 --> 00:41:29,490
+ثاني، هلأ أنا قولتلكوا إن الموضوع بيربط ما بين
+
+547
+00:41:29,490 --> 00:41:32,310
+الملاريا وما بين الـE.coli، بالنسبة للـE.coli
+
+548
+00:41:32,310 --> 00:41:36,350
+طبعا هي غنية عن التعريف، الـE.coli فيه حوالي أكثر من
+
+549
+00:41:36,350 --> 00:41:41,930
+700 سيروتايب من الـE.coli، عدد قليل منها ممكن يسبب
+
+550
+00:41:41,930 --> 00:41:44,830
+infection للإنسان، وطبعا بتعرفوا الـurine .. الـ
+
+551
+00:41:44,830 --> 00:41:47,290
+urinary tract infection يعني angiasis، الـ
+
+552
+00:41:47,290 --> 00:41:50,390
+gastrointestinal tract infection، ورفته الـ
+
+553
+00:41:50,390 --> 00:41:52,710
+enteropathogenic E coli والـenterohemorrhage، كل
+
+554
+00:41:52,710 --> 00:42:00,450
+هاي الأنواع بتسبب infection للإنسان، بالنسبة لغالبية
+
+555
+00:42:00,450 --> 00:42:03,370
+الأنظمة من هاي الـ700 سيروتايب، طبعا نفسهم بناء على
+
+556
+00:42:03,370 --> 00:42:06,550
+الـA والـK والـH antigen هي عبارة عن نورمال فلورة
+
+557
+00:42:06,550 --> 00:42:13,110
+موجودة في الـgastrointestinal tract، طيب، هلأ طبعا
+
+558
+00:42:13,110 --> 00:42:17,070
+مذكرينكوا أنه غير فوائد الـnormal flora أو بالتحديد
+
+559
+00:42:17,070 --> 00:42:21,110
+الـE.coli، أنها هي غير أنها بتعمل مقاومة للاستعمار بـ
+
+560
+00:42:21,110 --> 00:42:26,750
+infected microorganism أخرى، أيضا هي بتعمل على
+
+561
+00:42:26,750 --> 00:42:30,670
+إنتاج vitamin K، كمان بتعمل على .. بتساعد على
+
+562
+00:42:30,670 --> 00:42:35,510
+الامتصاص، اليوم اكتشفوا وظيفة جديدة أو الدراسة
+
+563
+00:42:35,510 --> 00:42:39,070
+اليوم بتحكي أنه في انها وظيفة جديدة تم اكتشافها للـ
+
+564
+00:42:39,070 --> 00:42:42,630
+E.coli أو بعض السلالات من الـE.coli الموجودة ك ..
+
+565
+00:42:42,630 --> 00:42:45,970
+كـnormal flora في الـgastrointestinal tract، هلأ
+
+566
+00:42:45,970 --> 00:42:50,330
+الدراسة بتقول أنه في ان بعض السلالات من الـE.coli
+
+567
+00:42:50,330 --> 00:42:56,410
+عندها القدرة على مقاومة مرض الملاريا، كيف؟ هلأ الـE
+
+568
+00:42:56,410 --> 00:43:00,630
+.coli، بعد الـstrength طبعا من الـequal لها بتقدر تنتج
+
+569
+00:43:00,630 --> 00:43:04,990
+sugar أو glycine، هذا الـglycine بتروح طبعا يحفز
+
+570
+00:43:04,990 --> 00:43:09,250
+جهاز المناعة أنه ينتج antibody ضده، طيب جهاز المناعة
+
+571
+00:43:09,250 --> 00:43:12,330
+نتج antibody ضده، ايش علاقته بالملاريا؟ الملاريا
+
+572
+00:43:12,330 --> 00:43:16,830
+وجدوا انها بتقدر تنتج نفس هذا الـsugar اللي هو
+
+573
+00:43:16,830 --> 00:43:21,230
+alpha galactosidase، بتعمل exploration لإله، بالتالي
+
+574
+00:43:21,230 --> 00:43:24,550
+يعني هلأ لما يكون فيه عنا .. عنا نورمال E.coli موجود
+
+575
+00:43:24,550 --> 00:43:28,530
+.. أنتجت .. ساعدت جهاز المناعة أنه ينتج Antibody،
+
+576
+00:43:28,530 --> 00:43:31,070
+ماشي، هلأ لما يصير فيه عنا .. فيه عنا كمية كافية
+
+577
+00:43:31,070 --> 00:43:33,150
+من الـAntibody، نفترض أنه فيه عنا كمية كافية من الـ
+
+578
+00:43:33,150 --> 00:43:36,030
+Antibody، لما يصير فيه عنا infection بالملاريا،
+
+579
+00:43:36,030 --> 00:43:39,170
+بده، فهي الـAntibody التي هي بالأصل مش تم تحفيزها
+
+580
+00:43:39,170 --> 00:43:41,730
+من ال .. من الملاريا التي إجت، لأ من السابق كان
+
+581
+00:43:41,730 --> 00:43:46,250
+فيه عنا Antibody من ال .. من الـE.coli، بدها تروح
+
+582
+00:43:46,250 --> 00:43:50,210
+تتهاجم الملاريا، طيب، أنا قولتلكوا رجزة ونقطة هي
+
+583
+00:43:50,210 --> 00:43:53,670
+أنه 90% من حالات الوفاة تسجلت كانت بموضوعها من
+
+584
+00:43:53,670 --> 00:43:56,310
+الأطفال، طب ليش؟ وبتخيّل لي أنها هي النقطة التي
+
+585
+00:43:56,310 --> 00:44:00,230
+لفتت انتباه العلماء أنهم يبحثوا في هات الموضوع، هلأ
+
+586
+00:44:00,230 --> 00:44:04,890
+وجدوا أنه الأطفال عندهم أو غير قادرين أو كمية الـ
+
+587
+00:44:04,890 --> 00:44:08,030
+antibody التي بتتم إنتاجها بوسطة هذا الـnormal
+
+588
+00:44:08,030 --> 00:44:11,010
+flora كمية قليلة جدا، غير قادرة على مقاومة علم اللي
+
+589
+00:44:11,010 --> 00:44:14,330
+عليه، الا هيك بنلاقي ان معظم حالات الوفاة بيكون من
+
+590
+00:44:14,330 --> 00:44:17,950
+الأطفال، وغير أنه في ناس أصلا بيكون عندهم
+
+591
+00:44:17,950 --> 00:44:20,990
+مثلا جهاز المناعة ضعيف، هاي حالات الوفاة بتتسجل
+
+592
+00:44:20,990 --> 00:44:24,790
+بالقدر، في ناس بيكون عندهم مثلا جهاز المناعة
+
+593
+00:44:24,790 --> 00:44:28,530
+ضعيف، أنه بالنهاية ما في كمية كافية من الـanti
+
+594
+00:44:28,530 --> 00:44:33,140
+-alpha galactosidase antibody، طيب طبعا بدهم وظفوا
+
+595
+00:44:33,140 --> 00:44:37,720
+هذا ال .. هذه الدراسة باشي طبعا بـunder vaccine فعلا
+
+596
+00:44:37,720 --> 00:44:41,640
+قدروا .. راحوا جابوا under .. قدروا يعملوا فاكسين
+
+597
+00:44:41,640 --> 00:44:46,340
+طبعا احنا بنحكي عن فاكسين يعني بيكون جزء من ال ..
+
+598
+00:44:46,340 --> 00:44:48,840
+من البكتيريا أو من البكتيريا .. أو البكتيريا كلها
+
+599
+00:44:48,840 --> 00:44:52,160
+فإنه راحوا حقنوه وجربوا على فران .. على الفئران
+
+600
+00:44:52,160 --> 00:44:56,680
+اللي هي nice test .. test، وبالفعل تمكنت الفئران
+
+601
+00:44:56,680 --> 00:45:01,100
+انها تقاوم مرض الملاريا، وحاليا بتتم التطوير
+
+602
+00:45:01,100 --> 00:45:04,360
+باستفادة من هذه النقطة انهم يطوروا vaccine لمرض
+
+603
+00:45:04,360 --> 00:45:10,980
+الملاريا، مثلا أنه إنهم يعملوا كجزء من جدول التطعيم
+
+604
+00:45:10,980 --> 00:45:14,280
+للأشخاص .. للمناطق الموبوءة بالملاريا، هذا شغلة،
+
+605
+00:45:14,280 --> 00:45:17,640
+شغلة ثانية، ممكن الأشخاص المسافرين الذين بدهم
+
+606
+00:45:17,640 --> 00:45:22,480
+ينتقلوا لمناطق الموبوءة بالملاريا، إنهم ياخذوا هذا
+
+607
+00:45:22,480 --> 00:45:27,600
+الـvaccine، خد بس، نقطة أخيرة بس بتعزز المفهوم إنه
+
+608
+00:45:27,600 --> 00:45:32,500
+احنا عارفين إنه الـnormal flora الموجودة في
+
+609
+00:45:32,500 --> 00:45:37,550
+أجسامنا بتختلف من، بتختلف في سكان المناطق .. بتختلف
+
+610
+00:45:37,550 --> 00:45:41,150
+من منطقة لأخرى في أجسام، يعني سكان منطقة سواء في الـ
+
+611
+00:45:41,150 --> 00:45:44,950
+.. سواء في الأنواع تبعها أو في الـrate تبعي ..
+
+612
+00:45:44,950 --> 00:45:48,670
+المعدل تبعها بتختلف، فهن وجدوا أنه الناس الذين
+
+613
+00:45:48,670 --> 00:45:51,670
+بيسافروا لمناطق الموبوءة بالـmalaria عندهم
+
+614
+00:45:51,670 --> 00:45:54,590
+susceptibility للإصابة بالـmalaria أكثر من الناس
+
+615
+00:45:54,590 --> 00:45:58,850
+الذين عايشين في .. مقيمين في نفس ال .. المنطقة
+
+616
+00:45:58,850 --> 00:46:02,620
+فيعني هاي الـnormal flora خلينا نقول أنه يعني أحد
+
+617
+00:46:02,620 --> 00:46:06,160
+الأسباب أنه هاي الـnormal flora موجودة بأجسامهم
+
+618
+00:46:06,160 --> 00:46:09,800
+في الـgastro intestinal tract كمية أكبر أو .. طيب
+
+619
+00:46:09,800 --> 00:46:15,460
+سبحان الله يعني .. هاي ربنا أعطاهم شغل أنهم متكيفين
+
+620
+00:46:15,460 --> 00:46:20,040
+مع البيئة التي عايشين فيها بالحقيقة
+
+621
+00:46:20,040 --> 00:46:24,320
+أنه بدي أتقدم بالشكر الجزيل للثلاثة الذين سبقوا
+
+622
+00:46:24,320 --> 00:46:27,380
+السبت الماضي في الـpresentation، وللأربعة الذين هذا
+
+623
+00:46:27,380 --> 00:46:31,310
+أيضا اللي اليوم تقدموه بأربع مواضيع، الستهيله
+
+624
+00:46:31,310 --> 00:46:35,090
+البكتيريا والـH-Pyri والـProbiotics والملاريا Anti
+
+625
+00:46:35,090 --> 00:46:40,430
+-coli، كل المواضيع فيها معلومات جديدة وشيقة ويمكن
+
+626
+00:46:40,430 --> 00:46:44,510
+ترجمتها إلى مقالات تُكتب على مجلة قوية علم الصحية
+
+627
+00:46:44,510 --> 00:46:49,050
+إن شاء الله لكن بدها جزئيات يعني اشتهدت فيها يعني
+
+628
+00:46:49,050 --> 00:46:53,790
+حضرتك H-Pylori مع الشهرة للراعاش هذه الجزئية هي
+
+629
+00:46:53,790 --> 00:46:57,510
+اللي أنا اكتف فيها الـ probiotic بدنا .. بدنا حاجة
+
+630
+00:46:57,510 --> 00:47:00,210
+جديدة ما شوفش حاجة جديدة أنا بالنسبة لي بدنا حاجة
+
+631
+00:47:00,210 --> 00:47:03,030
+جديدة الـ malaria والإيشي إيشي كلها الـ science
+
+632
+00:47:03,030 --> 00:47:08,330
+حاجات كتيرة محاضرة متنوعة عملها أنا عامة عن فوائد
+
+633
+00:47:08,330 --> 00:47:11,370
+الـ normal flora بس حقيقة أحد الفوائد بالفعل أن
+
+634
+00:47:11,370 --> 00:47:12,930
+they teach the immune system
+
+635
+00:47:18,880 --> 00:47:22,820
+بالضبط كمان نقطة إيه؟ نقطة مهمة جدا إذا قلت لكم إن
+
+636
+00:47:22,820 --> 00:47:26,240
+number of normal flora هو تقريبا
+
+637
+00:47:30,370 --> 00:47:33,570
+فـ you are more micro than a human يعني أنتم
+
+638
+00:47:33,570 --> 00:47:38,050
+ميكروبات أكثر منكم يعني طيب لو قلت لكم أنه عدد
+
+639
+00:47:38,050 --> 00:47:42,810
+الجينات و الـ contribution 150 لـ 1 يعني كلمة عدد
+
+640
+00:47:42,810 --> 00:47:45,150
+الجينات أقصد فيها أنواع الجينات اللي موجودة في
+
+641
+00:47:45,150 --> 00:47:49,990
+الميكروبات تنوعها 150 لـ 1 فالجينات برضه اللي بتنتج
+
+642
+00:47:49,990 --> 00:47:52,870
+من قبل هذه البكتيريا أكيد بتنتج أشياء مفيدة احنا 
+
+643
+00:47:52,870 --> 00:47:58,230
+مش عارفينها بس احنا للأسف البشر إذا بيعرفوا معلومة
+
+644
+00:47:58,230 --> 00:48:02,530
+بيستعدلوا في قطاف النتائج عرفنا أن البكتيريا مؤثر
+
+645
+00:48:02,530 --> 00:48:05,770
+الإنسان kill them all اقتل كل الخلايا اقتل كل
+
+646
+00:48:05,770 --> 00:48:10,870
+البكتيريا دمرها وبالتالي في الآخر قتلناها وبعد ما
+
+647
+00:48:10,870 --> 00:48:13,810
+قتلناها برة تظهر لنا أمراض أخرى لما كنا عارفينها من
+
+648
+00:48:13,810 --> 00:48:16,950
+قبل نتجي عن خلال الـ normal flora لحقيقة المعلومات
+
+649
+00:48:16,950 --> 00:48:20,790
+كلها جيدة الإلقاء كمان كان جيد من قبل الجميع سيانو
+
+650
+00:48:20,790 --> 00:48:24,550
+بكتيريا يعني يلا تكتب عن استخداماتها على استخدامات
+
+651
+00:48:24,550 --> 00:48:27,470
+الـ cyanobacteria لإنه برضه لها .. كنتوا عارفين إنه
+
+652
+00:48:27,470 --> 00:48:29,730
+زمان كانوا يقولوا لنا نباتات مهمة على كوكب الأرض
+
+653
+00:48:29,730 --> 00:48:34,410
+لتنتج oxygen نص الـ oxygen أو أكثر من نصه اللي
+
+654
+00:48:34,410 --> 00:48:37,490
+بينتج على كوكب الأرض هو من الـ cyanobacteria
+
+655
+00:48:37,490 --> 00:48:42,770
+وبالتالي احنا مدينون لهذه الـ bacteria بيش يعني
+
+656
+00:48:42,770 --> 00:48:46,550
+مدينون لها بالكثير مدينون لها بالكثير طيب احنا خلصنا الـ
+
+657
+00:48:46,550 --> 00:48:48,590
+presentation وقت انتهى فإن شاء الله بيشمل مرة
+
+658
+00:48:48,590 --> 00:48:49,230
+القادمة بإننا 
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/SVYwdKMMVYg_raw.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/SVYwdKMMVYg_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..51d085d2e5932b2d207447bf88bcd903c23c3767
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/SVYwdKMMVYg_raw.srt
@@ -0,0 +1,2632 @@
+1
+00:00:21,060 --> 00:00:23,860
+بسم الله الرحمن الرحيم اليوم ان شاء الله هنكمل مع
+
+2
+00:00:23,860 --> 00:00:30,820
+بعض تكملة ل ال antibiotics الجزء
+
+3
+00:00:30,820 --> 00:00:33,640
+السفلي هذا هو ال betidoglyca يعني اللي بتكون من
+
+4
+00:00:33,640 --> 00:00:36,980
+نام و ناج و بينهم glycosidic bond و بين النام و
+
+5
+00:00:36,980 --> 00:00:40,000
+النام في صفقة و طبقة فيها اللي هو ال cross link
+
+6
+00:00:40,000 --> 00:00:43,900
+هذا ال cell membrane بيتكون طبعا البكتيريا بتنمو و
+
+7
+00:00:43,900 --> 00:00:47,180
+تكاثر بذات استطيل فبالتالي بتكون محتاج جديدة من
+
+8
+00:00:47,180 --> 00:00:52,960
+مين؟ من ال betidoglycaتكونات واحدة فياندي حاسمة
+
+9
+00:00:52,960 --> 00:00:56,720
+أوتولايسين مرة بمعناها قبل هيك بتقص ال glycosidic
+
+10
+00:00:56,720 --> 00:01:01,740
+bond اللي بتلاحظه الآن هتفرق عشان يصير فياندي
+
+11
+00:01:01,740 --> 00:01:05,260
+واحدة incorporation ل new units صار فياندي
+
+12
+00:01:05,260 --> 00:01:08,600
+incorporation ل units لكن ماصار لسه فيه binding
+
+13
+00:01:08,600 --> 00:01:13,380
+ففياندي two two things مهمين جدا انه يتكون
+
+14
+00:01:13,380 --> 00:01:16,600
+glycosidic bond على اليابين واليسار ويتكون cross
+
+15
+00:01:16,600 --> 00:01:19,800
+link بين الاتنين فواحد اسمه انزيم
+
+16
+00:01:22,570 --> 00:01:26,730
+تراس بيبتيدايز اللي بيكون لـ cross-link و تراس
+
+17
+00:01:26,730 --> 00:01:32,930
+جليكوسيدايز اللي بيكون لـ glycosidic bond الأن في
+
+18
+00:01:32,930 --> 00:01:37,910
+أن هذا ال normal قلنا إيه؟ normal synthesis
+
+19
+00:01:37,910 --> 00:01:41,370
+للبيبتيدو إشرايكا بتعيدوا كمان مرة قبل ما ننتقل
+
+20
+00:01:41,370 --> 00:01:46,030
+إلى ال antibiotic اللي بتأثر على البيبتيدو إشرايكا
+
+21
+00:01:46,030 --> 00:01:50,390
+مناسبة ال membrane بيحصل في تصنيع ال units اللي هي
+
+22
+00:01:50,390 --> 00:01:56,230
+النام والناجبسلع ال transport الى ال biological
+
+23
+00:01:56,230 --> 00:02:01,970
+lab الاوتولاسين بيشتغل بيعمل كسر عشان يوسع و يدخل
+
+24
+00:02:01,970 --> 00:02:06,590
+يعمل incorporation ل new units لذلك بنلاقي ان ال
+
+25
+00:02:06,590 --> 00:02:09,590
+antibiotics ما اللي بتأثر على ال cell wall ما
+
+26
+00:02:09,590 --> 00:02:12,090
+بتأثر على ال fully grown bacteria بتأثر فقط على
+
+27
+00:02:12,090 --> 00:02:16,450
+تلك اللتي تم قسم اما اللي already grown ما بتأثر
+
+28
+00:02:16,450 --> 00:02:19,880
+انه مافيها biological synthesisصار في
+
+29
+00:02:19,880 --> 00:02:23,820
+incorporation useless إذا ما صار فيه robot ما بين
+
+30
+00:02:23,820 --> 00:02:26,960
+الجديد والجديد وما صار فيه cross-link هذا useless
+
+31
+00:02:26,960 --> 00:02:33,000
+فبتعرفي أنها قلنا ال glycosidic bond وبعدين
+
+32
+00:02:33,000 --> 00:02:35,580
+اللي هو ال Trans-peptidase بيعمل اللي هو ال cross
+
+33
+00:02:35,580 --> 00:02:38,280
+-link كده هذه الجزء الأول اللي هو ال normal
+
+34
+00:02:38,280 --> 00:02:42,580
+synthesis of peptidoglycan نشوفه مرتين، هنشوفه
+
+35
+00:02:42,580 --> 00:02:45,640
+كمان مرة تعالى نشوف الفنسينين
+
+36
+00:02:52,770 --> 00:03:00,930
+نفس النظام ستجد أن الخطوات تبعت ال normal
+
+37
+00:03:00,930 --> 00:03:04,770
+synthesis شغالة الفرق بين الأولى والثانية أن الأن
+
+38
+00:03:04,770 --> 00:03:09,410
+هناك الناس بنسلين البنسلين يشتغلونهيشتغل على الـ
+
+39
+00:03:09,410 --> 00:03:11,230
+cell wall صحيح لكن و لا بال cell wall بالظبط
+
+40
+00:03:11,230 --> 00:03:14,350
+هيشتغل على ال Trans ببتدايز زي ما هتشوفه الآن
+
+41
+00:03:14,350 --> 00:03:17,510
+هيعمل Blocking ايش هي ال Blocking؟ يعني هيجيب ال
+
+42
+00:03:17,510 --> 00:03:20,130
+Trans ببتدايز و يرتبط معاه يقول له انت الآن مالكاش
+
+43
+00:03:20,130 --> 00:03:24,370
+شغل يبقى مش هتكون a cross link و احنا متفقين منذ
+
+44
+00:03:24,370 --> 00:03:28,990
+بداية الحديث عن ال cell structure لما اتكلمنا ان
+
+45
+00:03:28,990 --> 00:03:32,130
+خلية بدون cross link او cell wall بدون cross link
+
+46
+00:03:32,130 --> 00:03:35,910
+هو cell wall يعني ضعيف جدا تشوف الآن هذه العملية
+
+47
+00:03:35,910 --> 00:03:40,480
+اللي تمتأتكوّن الـ glycosidic bond بالـ
+
+48
+00:03:40,480 --> 00:03:44,400
+Transglycosidase لكن ما اتكوّن اللي هو ال cross
+
+49
+00:03:44,400 --> 00:03:48,840
+link اذا الآن بما يتكوّن بال Transpeptidase لحظين
+
+50
+00:03:48,840 --> 00:03:52,320
+هاي Transpeptidase ايجا البنسالين اللي بيقولون
+
+51
+00:03:52,320 --> 00:03:56,740
+البنفسجي هيرتبط بمين بال Transpeptidase يعني مافيش
+
+52
+00:03:56,740 --> 00:04:00,660
+امكانية الآن انه يتكوّن cross link متفقنا عليها
+
+53
+00:04:00,660 --> 00:04:02,080
+النقطة
+
+54
+00:04:07,670 --> 00:04:11,670
+بالـ Transmit link ليش مهم الكلام هذا لأنه هنشوف
+
+55
+00:04:11,670 --> 00:04:16,250
+واحد تاني الآن اللي هو ال Vancomycin و ال
+
+56
+00:04:16,250 --> 00:04:18,490
+Vancomycin بيعدي لنفس النتيجة مش هتكون ورا cross
+
+57
+00:04:18,490 --> 00:04:27,370
+link لكن بطريقة مختلفة يعني
+
+58
+00:04:27,370 --> 00:04:30,510
+المفروض الآن لسه ال word synthesis صار لنا واضح هي
+
+59
+00:04:30,510 --> 00:04:38,660
+تالت مرة بنكرره تمام؟ كمان عننا الآن؟الـ Units
+
+60
+00:04:38,660 --> 00:04:50,560
+Transport الـ Auto-license هيشتغل يفسح مكان قدر
+
+61
+00:04:50,560 --> 00:04:53,680
+حضرت مين الآن ال Vanco Mycene اللي هو عبارة عن الـ
+
+62
+00:04:53,680 --> 00:04:59,580
+Glycopeptide ارتبط بالـ Sites اللي بيشتغل عليها
+
+63
+00:04:59,580 --> 00:05:02,360
+مين اللي بيشتغل عليها ال Trans-Peptidase
+
+64
+00:05:06,820 --> 00:05:10,580
+و الاصل ال transfer to days بيشبك هنا و بيكون ال
+
+65
+00:05:10,580 --> 00:05:13,420
+cross link لان بيجي ال transfer to days بده يشتغل
+
+66
+00:05:13,420 --> 00:05:17,880
+بلا جيش مكانه فارغ وبالتالي لن يستطيع ان يكون
+
+67
+00:05:17,880 --> 00:05:21,700
+cross link اذا في المحصلة ال vancelin و ال
+
+68
+00:05:21,700 --> 00:05:27,680
+vancomycin كلا هما اشملهم مانعوا ال cross link لكن
+
+69
+00:05:27,680 --> 00:05:30,980
+واحد ع طريق ال blocking ل transfer to days و واحد
+
+70
+00:05:30,980 --> 00:05:32,360
+blocking لل site
+
+71
+00:05:36,380 --> 00:05:43,160
+لكن في المحاصلة دلوقتي هم بيأثروا على ال cell wall
+
+72
+00:05:45,280 --> 00:05:48,080
+طيب أنا قولتلكوا المحاضرة الماضية إننا بنعمل بعض
+
+73
+00:05:48,080 --> 00:05:53,240
+أحيان modification صح؟ لل-antibiotic to extend the
+
+74
+00:05:53,240 --> 00:05:56,960
+spectrum ومثال الانبسيلين صار يؤثر على ال gram
+
+75
+00:05:56,960 --> 00:05:59,820
+positive ويؤثر على ال gram negative والانبسيلين
+
+76
+00:05:59,820 --> 00:06:03,780
+أخوه ال بنسيلين هل تأثير ال .. أو ال mechanism of
+
+77
+00:06:03,780 --> 00:06:07,080
+action تبع ال بنسيلين على ال gram negative هو نفسه
+
+78
+00:06:07,080 --> 00:06:11,220
+تأثير على ال gram positive بالتأكيد لأيبقى على
+
+79
+00:06:11,220 --> 00:06:14,340
+جرام نجدة كيف بدي يشتغل؟ بيشتغل على حالة اسمها
+
+80
+00:06:14,340 --> 00:06:17,000
+Penicillin Binding Proteins فهناك مجموعة من
+
+81
+00:06:17,000 --> 00:06:21,140
+البروتينات مهمة جدا إيه العلاقات اللي بتصنيها الـ
+
+82
+00:06:21,140 --> 00:06:25,880
+glucoglycan والـ cell wall فإذا كان ال Penicillin
+
+83
+00:06:25,880 --> 00:06:28,620
+Binding Proteins اللي طبطت بالPenicillin فبصير
+
+84
+00:06:28,620 --> 00:06:32,100
+تكسير لمين؟ لل cell wall تبع البكتيدة بطريقة
+
+85
+00:06:32,100 --> 00:06:37,860
+مختلفة عما حدث في الجرام نرجع
+
+86
+00:06:37,860 --> 00:06:44,500
+لل presentationو نذكركوا لما وصلنا احنا فاكرين
+
+87
+00:06:44,500 --> 00:06:48,590
+هذي؟ختمنا فيها احنا المرة الماضية المحاضرة تبعتنا
+
+88
+00:06:48,590 --> 00:06:52,930
+لما قلنا انه ال .. ال beta lactam ring اللي هي
+
+89
+00:06:52,930 --> 00:06:57,450
+الجزء أساسي من البنسالينات و من ال cephalosporins
+
+90
+00:06:57,450 --> 00:07:02,090
+تمام؟ و لذلك سمناهم ال cephalosporins و ال .. ال
+
+91
+00:07:02,090 --> 00:07:05,730
+.. ال بنسالين سمناهم beta lactam antibiotic إذا
+
+92
+00:07:05,730 --> 00:07:10,270
+صار فيه تكسير ال ring تبعت ال beta lactam صار ال
+
+93
+00:07:10,270 --> 00:07:12,470
+antibiotic اللي اسمه بنسالين صار اسمه penicillin
+
+94
+00:07:12,470 --> 00:07:15,170
+acid و فقد أي activity أو أي antimicrobial
+
+95
+00:07:15,170 --> 00:07:15,750
+activity
+
+96
+00:07:18,390 --> 00:07:21,110
+هو أخر من ال .. من ال antibiotics اللي أثرت سلوك
+
+97
+00:07:21,110 --> 00:07:23,730
+لها وزن كبير جدا في ال antibiotics اسمها
+
+98
+00:07:23,730 --> 00:07:28,130
+cephalosporins، cephalosporins الcephalosporins
+
+99
+00:07:28,130 --> 00:07:33,170
+أول ما اكتشفت لجوا أن التأثير تبعها يشبه إلى حد
+
+100
+00:07:33,170 --> 00:07:37,010
+كبير تأثير البنسالين، و هما التانين احنا قول معاهم
+
+101
+00:07:37,010 --> 00:07:38,770
+beta lactam-antibiotics
+
+102
+00:07:40,940 --> 00:07:44,280
+لما عملنا modification لهم وجدنا ان لا ممكن
+
+103
+00:07:44,280 --> 00:07:47,880
+تأثيرهم يمتد الى إجراءه نيجاتيب، لاحظوا الان في
+
+104
+00:07:47,880 --> 00:07:53,720
+اندي second, third and fourth generations، ايش
+
+105
+00:07:53,720 --> 00:07:56,380
+يعني second و third و fourth generation؟ انتوا
+
+106
+00:07:56,380 --> 00:08:00,850
+معاكم موبايلات اليوم اللي هي ال smart هذه؟في قبلها
+
+107
+00:08:00,850 --> 00:08:06,710
+كان موبايلات مش smart لجانب كاميرا و GPS قبلهم كان
+
+108
+00:08:06,710 --> 00:08:11,150
+مش عارف فيه أقل جودة فلما يكون فيه نقلة نوائية في
+
+109
+00:08:11,150 --> 00:08:15,610
+الحواسيب في ال antibiotics في الجوالات بنسميهم
+
+110
+00:08:15,610 --> 00:08:18,670
+أجيال الجيل الأول الجيل الثالث فال first
+
+111
+00:08:18,670 --> 00:08:21,270
+generation اللي هو ال natural ال first generation
+
+112
+00:08:21,270 --> 00:08:24,870
+اللي هو ال natural هذا بيأثر على الإجرام mostly
+
+113
+00:08:26,960 --> 00:08:30,520
+بأثر على كلا هما لكن تأثيرهم على الجراهم نيجاتيف
+
+114
+00:08:30,520 --> 00:08:34,900
+أفضل من اللي جراهم بوزتر وهنا يبقى نوّت إلى أن
+
+115
+00:08:34,900 --> 00:08:37,760
+الـSeparospulin الثالث والفاترين لايشن لهم
+
+116
+00:08:37,760 --> 00:08:41,580
+activity رائعة جدا على الـPseudomonas aeruginosa
+
+117
+00:08:42,470 --> 00:08:45,690
+بمعنى آخر احنا بنفتخر لما نلاقي antibiotic له
+
+118
+00:08:45,690 --> 00:08:51,190
+تأثير على pseudomonas aeruginosa لما لها من خبرة
+
+119
+00:08:51,190 --> 00:08:54,570
+سابقة احنا بنعرفها naturally resistance للبكتيري
+
+120
+00:08:54,570 --> 00:08:57,570
+لل antibiotic و can acquire resistance فبالتالي
+
+121
+00:08:57,570 --> 00:09:00,910
+لما نلاقي antibiotic effective عليها بنكون سعاداء
+
+122
+00:09:00,910 --> 00:09:04,270
+جدا من ضمن هذا ال antibiotic ال third وال fourth
+
+123
+00:09:04,270 --> 00:09:06,330
+generation cephalosporitis
+
+124
+00:09:11,560 --> 00:09:15,900
+أصبح إنه resistance عالي ضد هذه ال antibiotics
+
+125
+00:09:15,900 --> 00:09:21,680
+بتتلاحظولي ال beta-lactam اللي موجودة هنا نفسها هي
+
+126
+00:09:21,680 --> 00:09:24,560
+ال beta-lactam اللي موجودة هنا هذه عبارة عن
+
+127
+00:09:24,560 --> 00:09:28,500
+cephalosporin نوكلياس وهذه penicillin نوكلياس
+
+128
+00:09:28,500 --> 00:09:28,900
+تمام؟
+
+129
+00:09:34,170 --> 00:09:36,790
+اللي بتكسر ال beta-lactam ممكن نقول
+
+130
+00:09:36,790 --> 00:09:40,370
+cephalosporinase عنها إذا كانت بتستهدف ال
+
+131
+00:09:40,370 --> 00:09:43,790
+cephalosporine أو نقول penicillinase إذا بتستهدف
+
+132
+00:09:43,790 --> 00:09:48,130
+ال penicillin او كلا هما بيسميها و هذا الأسلم
+
+133
+00:09:48,130 --> 00:09:50,810
+الصحيح نسميها beta-lactamases
+
+134
+00:09:54,670 --> 00:09:56,530
+من ال Antibiotics الأخرى اللي بتأثر على ال cell
+
+135
+00:09:56,530 --> 00:09:59,370
+wall، Antibiotic مشهور بتعرفوه انتوا اسمه
+
+136
+00:09:59,370 --> 00:10:04,690
+Basteracin هذا ال Basteracin very toxic مابنقدراش
+
+137
+00:10:04,690 --> 00:10:08,930
+نستخدمه systemic يعني ناخده حبوب أو ناخده
+
+138
+00:10:08,930 --> 00:10:12,790
+injection فبيستخدم فقط topical، إذا ال Basteracin
+
+139
+00:10:12,790 --> 00:10:15,990
+بيستخدم إيه؟ إيش يعني topical؟ يعني جطرة يعني موضع
+
+140
+00:10:18,890 --> 00:10:21,950
+it is used against gram positive ولاحظوا كل ال
+
+141
+00:10:21,950 --> 00:10:25,010
+antibiotics اللي بتأثر على ال cell wall غالبا
+
+142
+00:10:25,010 --> 00:10:28,630
+أساسا على مين؟ على gram positive لإن هو الفعل ..
+
+143
+00:10:28,630 --> 00:10:31,590
+الموضوع ال cross link بالظبط مهم جدا له في هذا
+
+144
+00:10:31,590 --> 00:10:34,810
+الحالة ال vancomycin حكينا وشفناها و هو عبارة عن
+
+145
+00:10:34,810 --> 00:10:37,050
+ال glycopeptide و أنا قد كل مرة قلتلكوا أن هو the
+
+146
+00:10:37,050 --> 00:10:40,250
+last bullet هو الموضوع اللي مكتوب كل مرة the last
+
+147
+00:10:40,250 --> 00:10:45,380
+line against antibiotic resistant stuff الليأنا
+
+148
+00:10:45,380 --> 00:10:48,580
+قلت لكم من المرة الماضية أنه لأ البطالة الاصطناعي
+
+149
+00:10:48,580 --> 00:10:53,060
+لأنه أصلا لجينا البنك ومايسين intermediate أستفيد
+
+150
+00:10:53,060 --> 00:10:55,180
+الفوكاس أورس و لجينا البنك ومايسين resistant
+
+151
+00:10:55,180 --> 00:11:01,610
+أستفيد الفوكاس أورس فهنشوف لازم نورله على بدأطيب
+
+152
+00:11:01,610 --> 00:11:05,190
+في كمان two drugs بيعتبروهم cell wall inhibitors
+
+153
+00:11:05,190 --> 00:11:09,390
+لكن ليس لهم علاقة بذاكر البكتيريا فقط بنوع أخر من
+
+154
+00:11:09,390 --> 00:11:14,290
+البكتيريا اللي هي البكتيريا المسمى mycobacteria
+
+155
+00:11:14,290 --> 00:11:19,090
+لذلك سميناهم Anti Mycobacterium Antibiotics ليش
+
+156
+00:11:19,090 --> 00:11:22,890
+خصصنا Anti Mycobacterium لأن الحقيقة تانية دولة
+
+157
+00:11:22,890 --> 00:11:24,810
+استخداهم فقط مقصود
+
+158
+00:11:28,500 --> 00:11:32,360
+على بكتيريا أخرى غير اللي هو ال micro bacteria
+
+159
+00:11:32,360 --> 00:11:36,360
+يعني مثلا ال streptomycin معروف عنه إله تأثير على
+
+160
+00:11:36,360 --> 00:11:39,020
+ال micro bacteria وعلى other bacteria لكن
+
+161
+00:11:39,020 --> 00:11:42,240
+ماسمّيناه antimicrobacterium antibacterium لماذا؟
+
+162
+00:11:42,240 --> 00:11:48,020
+لأنه مش exclusive بسبب تنين exclusive و أذكركم
+
+163
+00:11:48,020 --> 00:11:53,400
+فيهم أنا بشكل سريع لأن روحنا نطلع على اللوحة اللي
+
+164
+00:11:53,400 --> 00:11:55,220
+فيها ال broad spectrum و ال neuroscience
+
+165
+00:11:57,900 --> 00:12:01,080
+الإيزونيازايد اللى .. اللى الان نتكلم عنه احنا هو
+
+166
+00:12:01,080 --> 00:12:04,240
+اللى موجود تحت ال microbacterium بينما ال
+
+167
+00:12:04,240 --> 00:12:06,960
+streptomycin هيوا microbacterium و ال gram نيجاتير
+
+168
+00:12:06,960 --> 00:12:11,980
+فما بيعملوا antimicrobacterium فقط تلك اللى مخصصة
+
+169
+00:12:11,980 --> 00:12:17,940
+exclusive للنوع هذا نرجع برجوها و نشوف ال
+
+170
+00:12:17,940 --> 00:12:21,400
+antimicrobacterium اللى هم التانية الإيزونيازايد
+
+171
+00:12:25,160 --> 00:12:29,200
+الذي أحدث ثورة في علاج ال micro bacterium
+
+172
+00:12:29,200 --> 00:12:33,760
+tuberculosis قبل اكتشاف هذا ال antibiotic كان علاج
+
+173
+00:12:33,760 --> 00:12:37,960
+ال tuberculosis تقريبا شبه مستحيل او يستغرق تلت
+
+174
+00:12:37,960 --> 00:12:41,700
+سنوات او اربع سنوات بال antibiotics الأخرىلما تم
+
+175
+00:12:41,700 --> 00:12:46,740
+اكتشاف هذا ال antibiotic وصلنا لمرحلة انه نعالج ال
+
+176
+00:12:46,740 --> 00:12:50,520
+tuberculosis في ستة أشهر، في ستة أشهر، هذا هو
+
+177
+00:12:50,520 --> 00:12:55,080
+الرقم قياسي للmycobacterium tuberculosis هذا يعني
+
+178
+00:12:55,080 --> 00:12:57,680
+انهيبشن لـmycolic acid، وكلكوا بتعرفوا ان
+
+179
+00:12:57,680 --> 00:13:01,820
+البكتيريا تتميز بـwaxy lipids اللي هو الmycolic
+
+180
+00:13:01,820 --> 00:13:06,000
+acid بدونه هي ايه تتميز بشيء، وبالتالي هذا ال
+
+181
+00:13:06,000 --> 00:13:09,460
+antibiotic منع تصنيع الmycolic acid وجهة منع ال
+
+182
+00:13:09,460 --> 00:13:14,250
+replication تبعهالتاني ال ethambutol بمنعش تصنيع
+
+183
+00:13:14,250 --> 00:13:17,770
+ال mycolic acid لكن بيمنع ال incorporation تبعه،
+
+184
+00:13:17,770 --> 00:13:20,770
+ايش incorporation؟ يعني انا هاي ال mycolic acid
+
+185
+00:13:20,770 --> 00:13:24,510
+صنعته، لازم اركبه في مكانه، هان بيمنع تركيب ال
+
+186
+00:13:24,510 --> 00:13:27,390
+mycolic acid في مكانه، وفي المحصلة النهائية
+
+187
+00:13:27,390 --> 00:13:33,250
+التانية مافيش mycolic acid، مافيش mycolic acidنجي
+
+188
+00:13:33,250 --> 00:13:37,510
+لنوع تاني من ال drugs اللي هم ال ال ال ال protein
+
+189
+00:13:37,510 --> 00:13:40,370
+synthesis inhibitors و يا ريت قبل ما نطلع على
+
+190
+00:13:40,370 --> 00:13:43,830
+اللوحة بنحاول نعرف برضه بعض اللي هو ال animation
+
+191
+00:13:43,830 --> 00:13:44,810
+كتير مهمين
+
+192
+00:14:10,730 --> 00:14:14,470
+ننظر لو حنشيرها للآن، عندنا اللي هو ال .. ال
+
+193
+00:14:14,470 --> 00:14:18,910
+macrolytes ال macrolytes هي مجموعة chemical group
+
+194
+00:14:18,910 --> 00:14:22,530
+من ال antibiotics تشمل مجموعة من ال .. من ال
+
+195
+00:14:22,530 --> 00:14:25,570
+antibiotics برضه المهمة زي الارتروميسين،
+
+196
+00:14:25,570 --> 00:14:29,030
+أزتروميسين وكليارتروميسين، هيموروا معانا في ال
+
+197
+00:14:29,030 --> 00:14:32,050
+presentation ارتروميسين، أزتروميسين
+
+198
+00:14:32,050 --> 00:14:35,550
+وكليارتروميسين، مجموعة مهمة بتيجي الصحيح من ال ..
+
+199
+00:14:35,550 --> 00:14:39,840
+من ال antibioticsوالان لما نتكلم على مجموعة بتأثر
+
+200
+00:14:39,840 --> 00:14:43,100
+على ال protein synthesis قسمناهم سابقا الى 50S
+
+201
+00:14:43,100 --> 00:14:45,840
+Antibiotics و 30S Antibiotics يعني اللي بتأثر على
+
+202
+00:14:45,840 --> 00:14:49,360
+ال 50S قد يتأثر على ال 30S حتى اللي بيأثر على ال
+
+203
+00:14:49,360 --> 00:14:53,180
+50S بيأثره بطريقة و طرق مختلفة و متنوعة فحشوف مع
+
+204
+00:14:53,180 --> 00:14:58,760
+بعض ان شاء الله كيف بيشتغل حضرة اللي هو ال
+
+205
+00:14:58,760 --> 00:15:04,080
+erythromycin او ال macroهذه أول خطوة ان في ال
+
+206
+00:15:04,080 --> 00:15:07,040
+translation في ال translation أول خطوة انه يتكون
+
+207
+00:15:07,040 --> 00:15:10,140
+ال initiation complex المكوّن منها كان من أنصر
+
+208
+00:15:10,140 --> 00:15:14,660
+واحد mRNA من ال five to three prime من ال 30S من
+
+209
+00:15:14,660 --> 00:15:19,180
+هذا ال ال ال amino acrylate RNA اللي حامل ميثيونين
+
+210
+00:15:19,180 --> 00:15:22,540
+اللي حامل ميثيونين اللي هو اللي تبع اللي اللي
+
+211
+00:15:22,540 --> 00:15:25,360
+بيحمل ال anti codon تبع ال initiation ال
+
+212
+00:15:25,360 --> 00:15:30,280
+initiation codon صح؟ و ال 50S يبقى في عندي صار هذا
+
+213
+00:15:30,280 --> 00:15:31,480
+بيسموه ال boson complex
+
+214
+00:15:34,240 --> 00:15:41,980
+الخطوة التانية هي انه يجيني one new tRNA يعني عكار
+
+215
+00:15:41,980 --> 00:15:46,160
+الـ tRNA ويضرب حمضته في السلسلة فهي تطلع ال
+
+216
+00:15:46,160 --> 00:15:50,940
+macroarray للأسف ارتباط في المنطقة هاي في ال 50s
+
+217
+00:15:50,940 --> 00:15:59,190
+هذا الارتباطش بيعمل بيؤدي إلى ان اي newلأ مرتفع،
+
+218
+00:15:59,190 --> 00:16:02,990
+بس اللي جابله .. اللي جابله بدي فارغ الحمول تبعته
+
+219
+00:16:02,990 --> 00:16:05,570
+من ال amino acids، هذا بيمنع ما يسمى بالـ
+
+220
+00:16:05,570 --> 00:16:09,810
+Translocation، لينتقال ال amino acid إلى السلسل أو
+
+221
+00:16:09,810 --> 00:16:11,710
+السلسل إلى ال amino acid جديد
+
+222
+00:16:14,820 --> 00:16:19,160
+أقول بفرج و حمولته و بمشي بدون ما يتكون سلسلة من
+
+223
+00:16:19,160 --> 00:16:24,960
+ال polypeptide صحيح يعني يبقى معنا الحكي انا في
+
+224
+00:16:24,960 --> 00:16:28,660
+عندي انهيبش ل ال protein synthesis كمحصلة كيف عن
+
+225
+00:16:28,660 --> 00:16:33,500
+طريق انهيبش اللي بتديه ال transferase اللي هو
+
+226
+00:16:33,500 --> 00:16:37,980
+trans location فبنشوف
+
+227
+00:16:44,670 --> 00:16:52,090
+التالتة بيساكلين بيشتغل
+
+228
+00:16:52,090 --> 00:16:54,950
+على ال 50S بيشتغل على 30S مش على 50S
+
+229
+00:16:59,570 --> 00:17:04,030
+كمان مرة translation من البداية لازم نكون عارفين
+
+230
+00:17:04,030 --> 00:17:08,150
+ان ال تتراثاكليين بيرتبط فقط في وين في ال 30S وهيك
+
+231
+00:17:08,150 --> 00:17:12,130
+وشايفين هنا شكله وين جال فبعمل bulging، bulging
+
+232
+00:17:12,130 --> 00:17:14,690
+يعني نوع من النتوء في ال .. في ال .. في ال
+
+233
+00:17:14,690 --> 00:17:17,830
+ribosome this prevents the binding of the amino
+
+234
+00:17:17,830 --> 00:17:20,490
+alkyl tRNA اللي هو ال charge .. كلمة charged ايش
+
+235
+00:17:20,490 --> 00:17:25,170
+يعني charge؟ مشحون يعني حامل amino acid to the A
+
+236
+00:17:25,170 --> 00:17:28,920
+-site اللي هو acceptor siteأيه المفروض ان هو
+
+237
+00:17:28,920 --> 00:17:34,080
+الانتيكودون صح؟ ما نعمل ارتباط يعني ماعرف احكي انه
+
+238
+00:17:34,080 --> 00:17:37,700
+it will not accept new amino acids اذا كده كان ال
+
+239
+00:17:37,700 --> 00:17:42,040
+protein المخلق فيه اربع خمس amino acids هيوجب و
+
+240
+00:17:42,040 --> 00:17:45,700
+انتهى الموضوع مافيش ان ال protein احنا هكذا بيطلع
+
+241
+00:17:45,700 --> 00:17:48,920
+عشوائي لازم ال protein يكون له ايه لازم يكون له
+
+242
+00:17:48,920 --> 00:17:53,360
+تسلسل معين مبني على ال mRNA اللي موجودة بتاعي
+
+243
+00:17:53,360 --> 00:17:55,120
+هيكون non-functional
+
+244
+00:17:58,480 --> 00:18:01,700
+طب فيه هنا كامل حاجة تالية let me know if you like
+
+245
+00:18:01,700 --> 00:18:02,120
+both sides
+
+246
+00:18:17,350 --> 00:18:22,750
+الـ Amino Glycosides برضه ترتبط بالـ 30S يبقى كام
+
+247
+00:18:22,750 --> 00:18:26,570
+واحد بالـ 30S بتعرفين تترا ساكلينز واللي هو الـ
+
+248
+00:18:26,570 --> 00:18:30,050
+Amino Glycosides هاي اتنين 30S تفضلنا ناخد كلمة
+
+249
+00:18:30,050 --> 00:18:35,250
+لإتنين 50S سهلين الـ Chloramphenicol زي
+
+250
+00:18:35,250 --> 00:18:37,710
+الماكرولايتز الماكرولايتز اللي شوفناه قبل شويه
+
+251
+00:18:37,710 --> 00:18:41,330
+اللي يقول عن الارتراوكميسي تبتطلع مع بعض على اللي
+
+252
+00:18:41,330 --> 00:18:45,190
+هو النمل بلاي عشان نشوف كيف ال Amino Glycoside
+
+253
+00:18:45,190 --> 00:18:55,290
+بيشتربنجر طبط وين برضه في ال 30s يبجي
+
+254
+00:18:55,290 --> 00:18:58,730
+عمل earlier termination لمين مش المفروض انه هذا
+
+255
+00:18:58,730 --> 00:19:03,370
+العمل يتوجف متى لما نشوف start كوده فهذا هو كأنه
+
+256
+00:19:03,370 --> 00:19:10,950
+عمل بدل start كوده عمل كأنه start كوده انا نزلت
+
+257
+00:19:10,950 --> 00:19:14,570
+مدرسة على ال Facebook مبالح المولودين في ملف مضغوط
+
+258
+00:19:19,450 --> 00:19:22,590
+لأ ممكن هو اللي قلته زي ما بعض الأحيان بصير عند
+
+259
+00:19:22,590 --> 00:19:28,230
+بعض الأشكال تفتح الملفات اعمل open with واختاري
+
+260
+00:19:28,230 --> 00:19:33,090
+اللي هو ال internet explorer غالبا بيفتح ان شاء
+
+261
+00:19:33,090 --> 00:19:33,690
+الله معاك الفيديو
+
+262
+00:19:39,050 --> 00:19:42,570
+إذا اتفقنا على إنه المجموعة اللي بتوقف تصنيع ال
+
+263
+00:19:42,570 --> 00:19:45,830
+protein synthesis في منها بيشتغل ال 50S و بيشتغل
+
+264
+00:19:45,830 --> 00:19:50,390
+ال 30S ناخد أشهرهم ال chloramphenicol اللي كان في
+
+265
+00:19:50,390 --> 00:19:54,010
+يوم من الأيام كان في يوم من الأيام drug of choice
+
+266
+00:19:54,010 --> 00:19:56,750
+لعلاج كثير من الأمراض منها التيفويت فيها
+
+267
+00:20:00,980 --> 00:20:05,640
+باستمرار وبنستفيد من المعلومات تبعتنا لاجينا إنه
+
+268
+00:20:05,640 --> 00:20:09,760
+الناس اللي بياخدوا ال chrome phenicol لفترة طويلة
+
+269
+00:20:09,760 --> 00:20:12,400
+بيصير عندهم bone marrow depression وممكن يصير
+
+270
+00:20:12,400 --> 00:20:15,300
+عندهم نوع من ال anemia اسمه plastic anemia
+
+271
+00:20:15,300 --> 00:20:20,340
+وبالتالي ال chrome phenicol تم إيقاع استخدامه
+
+272
+00:20:20,340 --> 00:20:24,760
+systematical systematical يعني oral أو injectionو
+
+273
+00:20:24,760 --> 00:20:29,460
+فقط مقصود استخدامه الآن على typical applications
+
+274
+00:20:29,460 --> 00:20:36,080
+مثل ما شاهدنا قبل برود سبكترام وسط تايف .. نستخدمه
+
+275
+00:20:36,080 --> 00:20:39,380
+بعض الحين في المقتبع اللي بنضيفه على ال media لقتل
+
+276
+00:20:39,380 --> 00:20:41,840
+الببتيرة لأنه برود سبكترام فاللي ابقى بدأ ألمي
+
+277
+00:20:44,500 --> 00:20:47,320
+خلرانفينيكولى على الوسط أسابقته المين البكتيريا
+
+278
+00:20:47,320 --> 00:20:51,800
+عشان نسمع لها الفيطريات تانية it binds 50S
+
+279
+00:20:51,800 --> 00:20:54,980
+-ribosomal subunit inhibits peptide bond formation
+
+280
+00:20:54,980 --> 00:20:58,760
+of television protein synthesis الأمينoglycosides
+
+281
+00:20:58,760 --> 00:21:02,080
+أخذنا أمثلة أو عليها أمثلة streptomycin نيوميسين
+
+282
+00:21:02,080 --> 00:21:06,120
+and جنتاميسين خليني أحكي شوية عن المجموعة دي لإن
+
+283
+00:21:06,120 --> 00:21:08,500
+هي مجموعة تتغير من المجموعات المهمة برضه في ال
+
+284
+00:21:08,500 --> 00:21:14,920
+antibioticsهذه المجموعة تتميز بسميتها العالية اللي
+
+285
+00:21:14,920 --> 00:21:18,460
+هي سمية على الإنسان و بيسموها the three toxicities
+
+286
+00:21:18,460 --> 00:21:24,200
+السميات auto, nephro and neuro auto اللي هي العصب
+
+287
+00:21:24,200 --> 00:21:30,460
+السمعي و نفره اللي هي الكيليا و ال neuro ال
+
+288
+00:21:30,460 --> 00:21:35,410
+toxicity اللي هي العصب بشكل عامهذه انا بتقولها من
+
+289
+00:21:35,410 --> 00:21:39,710
+نموذج نموذج ليش احنا في التحاليل الطبية او في
+
+290
+00:21:39,710 --> 00:21:43,390
+الصيدلة القصر ما نوصفش للناس قد يحط لو احنا اللي
+
+291
+00:21:43,390 --> 00:21:47,640
+عزلنا ال microbe ع مدار ال sensitivity testingأحنا
+
+292
+00:21:47,640 --> 00:21:50,960
+اللي بنعرف عن المريض حاجة واحدة العينة اللي
+
+293
+00:21:50,960 --> 00:21:54,700
+أخدناها منه والنتيجة تبعتنا صح هذا لأ لكن الطبيب
+
+294
+00:21:54,700 --> 00:21:58,100
+بيعرف عن المريض معلومات أكتر بيعرف symptoms و
+
+295
+00:21:58,100 --> 00:22:01,700
+بيعرف history لو كان المريض هذا عنده kidney
+
+296
+00:22:01,700 --> 00:22:04,480
+failure أو بداية kidney failure أو قصور في الكلية
+
+297
+00:22:04,480 --> 00:22:08,760
+الجرعة اللي بتعطيها من ال aminoglycosides هيجب أن
+
+298
+00:22:08,760 --> 00:22:14,440
+تكون جرعة أصغر لأن ال elimination لهذا ال drug
+
+299
+00:22:14,440 --> 00:22:19,040
+بيكون عبر الكليةطب إذا الكلية أصلا فيها قصور فال
+
+300
+00:22:19,040 --> 00:22:21,440
+elimination مش هيكون طبيعي وبالتالي هيزيد عمره
+
+301
+00:22:21,440 --> 00:22:25,180
+واحد في الدم، إذا زد عمره في الدم و أصلا بيعمل
+
+302
+00:22:25,180 --> 00:22:28,400
+nephrotoxicity يبقى هيزيده من إحدة لإحدة للكلية
+
+303
+00:22:28,400 --> 00:22:31,120
+بزيادة، هو عنده أصلا مشكلة و احنا بنضيفله على
+
+304
+00:22:31,120 --> 00:22:35,440
+مشكلة ضعفة جديدة، بندمره بقالين في الكلية، لذلك
+
+305
+00:22:35,440 --> 00:22:39,440
+إياكمفي يوم من الأيام هتصرف ما يمكن توصفه
+
+306
+00:22:39,440 --> 00:22:42,860
+Antibiotics لإنه يبقى لكوا في بعض يعني الصورة غير
+
+307
+00:22:42,860 --> 00:22:45,780
+مكتملة زي الكلام اللي احنا حكيناه عجب شوية لما
+
+308
+00:22:45,780 --> 00:22:48,640
+الواحد بيرحل على معلومة و بيستغلها بيستعجل في
+
+309
+00:22:48,640 --> 00:22:54,260
+استغلالها قد تؤذي الاستعجال او لما تكون مبنية او
+
+310
+00:22:54,260 --> 00:22:57,940
+القرار مبني على معلومات قليلة للأسف يكون القرار مش
+
+311
+00:22:57,940 --> 00:23:00,900
+جيد لكن لما تكون معلومة مبنية على صورة كاملة لما
+
+312
+00:23:00,900 --> 00:23:01,540
+تكون القرار
+
+313
+00:23:08,340 --> 00:23:15,080
+لوصف علاجات استراتومايسين نيومايسين and جنتومايسين
+
+314
+00:23:15,080 --> 00:23:19,000
+هذه المجموعة .. أفضل أنا قلت 3 منهم لكن هم يمكن
+
+315
+00:23:19,000 --> 00:23:23,220
+أكتر من 20 أكتر من 20 كلهم بتميزوا بسميات .. لكن
+
+316
+00:23:23,220 --> 00:23:28,040
+سمياتهم مختلفة يعني مثلا الجنتومايسين مستخدم لحد
+
+317
+00:23:28,040 --> 00:23:34,720
+اليوم بشكل كبير الكنامايسين الاميكاسينأسمع بي
+
+318
+00:23:34,720 --> 00:23:37,780
+ممنوع على الصبورة، لكن much more safer than
+
+319
+00:23:37,780 --> 00:23:41,500
+neomycin، neomycin is not given systematically
+
+320
+00:23:41,500 --> 00:23:46,320
+بالمرة، ممنوع يُعطى، فلازم مرنجلانين لل typical،
+
+321
+00:23:46,320 --> 00:23:49,500
+ده كام واحد typical أخدته اليوم، مين حافظهم؟
+
+322
+00:23:54,150 --> 00:23:58,630
+وهي نيوميسين و هناخد واحد كمان شوية اللي هو
+
+323
+00:23:58,630 --> 00:24:03,650
+بوليمكسين بيه بتاع ال cell membrane وبالتالي بيكون
+
+324
+00:24:03,650 --> 00:24:07,490
+هيك ألفنا مجموعة من ال antibiotic استخدامها فقط
+
+325
+00:24:07,490 --> 00:24:11,050
+مقصور على مين على ال typical application يبدو أنه
+
+326
+00:24:11,050 --> 00:24:15,650
+اليوم ان شاء الله هنكمل مع بعض روض لي مجموعة من
+
+327
+00:24:15,650 --> 00:24:20,330
+الطالبات و نبدأ مع طالبة همسة تعفش بموضوع
+
+328
+00:24:26,060 --> 00:24:29,680
+السلام عليكم اليوم بحكي لكم عن بكتيريا اسمها
+
+329
+00:24:29,680 --> 00:24:32,940
+cyanobacteria هاي ال cyanobacteria بتتميز لأن
+
+330
+00:24:32,940 --> 00:24:35,440
+عندها اللونين هو ال green و ال blue السبب
+
+331
+00:24:35,440 --> 00:24:38,940
+باكتشافها ان كانوا زمان أول ما وجدوها حكوا عنها
+
+332
+00:24:38,940 --> 00:24:41,880
+انها تحالب ليش حكوا عنها تحالب بسبب وجود هدولة
+
+333
+00:24:41,880 --> 00:24:47,560
+اللونين اليوم بدأتحكوا كمان عن ان هي بكتيريا قديمة
+
+334
+00:24:47,560 --> 00:24:54,510
+جداموجودة حيث قالت بعد البحثات ان الكركب موجودة من
+
+335
+00:24:54,510 --> 00:24:58,270
+اتنين one تمانية billion years اذا ان هي موجودة من
+
+336
+00:24:58,270 --> 00:25:02,730
+ألاف السنين من الملايين حتى كمان السنين هي طبعا
+
+337
+00:25:02,730 --> 00:25:06,310
+موجودة بتقدر تعيش في الميه بتقدر تعيش في السولات
+
+338
+00:25:06,310 --> 00:25:09,610
+بتقدر تعيش حاليا ال skin smear وموجودة كمان في بعض
+
+339
+00:25:09,610 --> 00:25:12,850
+الخضروات موجودة كمان في ال switch كمان ان هي
+
+340
+00:25:12,850 --> 00:25:15,770
+موجودة
+
+341
+00:25:17,480 --> 00:25:21,080
+أول ما بدوا انهم يكتشفوها .. اكتشفوها في دول
+
+342
+00:25:21,080 --> 00:25:25,980
+اسكندرفية خاصة دولة فنلاندا، دولة فنلاندا كانوا
+
+343
+00:25:25,980 --> 00:25:30,260
+لما اكتشفوها لاحظوا انه في ارتفاع درجات الحرارة في
+
+344
+00:25:30,260 --> 00:25:32,780
+الميه، لما اكتشفوا انه في ارتفاع درجات الحرارة في
+
+345
+00:25:32,780 --> 00:25:36,320
+الميه فقدروا انه موجود هذه البكتيريا وكمان ارتفاع
+
+346
+00:25:36,320 --> 00:25:40,880
+درجات الحرارة في الميه بتساعدها على نموها بشكل
+
+347
+00:25:40,880 --> 00:25:46,880
+كبيرحيث انتحار .. و هلأ في عندها تلات أنواع أول
+
+348
+00:25:46,880 --> 00:25:52,040
+نوع اللي هو نوع أخضر وجود النوع الأخضر بسبب وجود
+
+349
+00:25:52,040 --> 00:25:54,760
+mad chlorophyll اللي بيساعدها في عملية البناء
+
+350
+00:25:54,760 --> 00:25:58,620
+الضوءي و أيضا في عندنا كمان لون أحمر اللون الأحمر
+
+351
+00:25:58,620 --> 00:26:02,460
+اكتشفوه في طائر اسمه ال flamingo هذا الطائر ال
+
+352
+00:26:02,460 --> 00:26:05,440
+flamingo لما كان بده يشرب .. لما بيشرب من هذه
+
+353
+00:26:05,440 --> 00:26:11,480
+الميه بيصير لون جسمه سبانك فعشان هيك وجود لون أحمر
+
+354
+00:26:11,480 --> 00:26:18,530
+او بكتيريا حمراءأما اللون الأزرق فهو طبعا سبب بعض
+
+355
+00:26:18,530 --> 00:26:21,490
+الصبغات، هنا أنا بدأ أحكيلكوا أنا عن بعض صفاتها
+
+356
+00:26:21,490 --> 00:26:24,630
+اللي هو عبارة عن ground water tap و هي طبعا
+
+357
+00:26:24,630 --> 00:26:29,310
+obligate و هي motility تحت ال microscope و هي
+
+358
+00:26:29,310 --> 00:26:32,830
+حجمها كتير صغير uncellular و هي مافيهاش
+
+359
+00:26:35,430 --> 00:26:40,130
+هنا بس بقيت أحكيلكوا قبل ما نحكي عن ال .. الميادات
+
+360
+00:26:40,130 --> 00:26:44,970
+تبعتها بقيت أحكي عن بعض المساورت تبعتها عشان
+
+361
+00:26:44,970 --> 00:26:49,330
+الميه، لما احنا حكينا إن كانت موجودة في الميه
+
+362
+00:26:49,330 --> 00:26:54,830
+بتعمل ارتفاع درجات الحرارة، أي إنسان لما بت .. بعد
+
+363
+00:26:54,830 --> 00:26:57,770
+المساورة كانت تظهر على المنازلين هناك، أي واحد روح
+
+364
+00:26:57,770 --> 00:27:03,610
+يسبح، كانت بيسيل في عندهحساسية على الجلد أو بعض
+
+365
+00:27:03,610 --> 00:27:07,770
+الكهابات في العين وكمان هذه البكتيريا كانت بتغذى
+
+366
+00:27:07,770 --> 00:27:11,990
+على مادة الفسفور الموجودة في النباتات، في التحالب،
+
+367
+00:27:11,990 --> 00:27:18,610
+تحالب ميتة أو بعض الأسماك هناك لما الانسان كمان
+
+368
+00:27:18,610 --> 00:27:22,650
+ممكن بده ياكل مأكولات بحرية خاصة من دولة فنلاندا
+
+369
+00:27:22,650 --> 00:27:26,730
+كان بيصير عنده دياريا وكانت بيصير في عنده صداع
+
+370
+00:27:26,730 --> 00:27:30,290
+شديد و أيضا درجات الحرارة، برتفع عنده درجة الحرارة
+
+371
+00:27:31,520 --> 00:27:36,020
+رغم انها .. انها طبعا مضرة الا انها مفيدة جدا
+
+372
+00:27:36,020 --> 00:27:40,900
+للبيئة، اول فائدة انها للبيئة ان هي كانت بتقدر
+
+373
+00:27:40,900 --> 00:27:44,800
+تستطيع تحكي مادة السريلوز الموجودة فيه النباتات او
+
+374
+00:27:44,800 --> 00:27:48,540
+الخشب انها لما تحكي مادة السريلوز في النباتات
+
+375
+00:27:48,540 --> 00:27:55,010
+بيساعدها علىتشكيل بتساعد النباتات بتشكل هيكل
+
+376
+00:27:55,010 --> 00:28:01,270
+النبات بيعطيها المرونة بيعطيها الصلابة وفي برضه
+
+377
+00:28:01,270 --> 00:28:07,170
+باحث بيحكي انه تلتين من هذه البكتيريا بتعملي على
+
+378
+00:28:07,170 --> 00:28:11,230
+تثبيت النيتروجين هو nitrogen fixation هنا عندنا
+
+379
+00:28:11,230 --> 00:28:15,730
+رسمة مختصرة عن تثبيت النيتروجين احنا عارفين ان
+
+380
+00:28:15,730 --> 00:28:19,600
+النيتروجين موجود في الجوو كتير من التربة مافيهاش
+
+381
+00:28:19,600 --> 00:28:22,220
+خالي هو من ال nitrogen فهذا عندى انا النوع من
+
+382
+00:28:22,220 --> 00:28:29,020
+البكتيريا انه بيساعد البكتيريا الموجودة
+
+383
+00:28:29,020 --> 00:28:31,840
+في التربة بتساعد بعض البكتيريا على انها تستطيع
+
+384
+00:28:31,840 --> 00:28:35,920
+تثبيت ال nitrogen عندنا هون بكتيريا تثبيت ال
+
+385
+00:28:35,920 --> 00:28:41,010
+nitrogen اول حاجة بتاخد ال nitrogen من الجمعفهذه
+
+386
+00:28:41,010 --> 00:28:44,530
+البكتيرا بتقوم بتثبيته، بتحوله إلى أمونيوم، بعد
+
+387
+00:28:44,530 --> 00:28:48,690
+هيك بتكون عندها بكتيرا اسم بكتيرا منترجة، بتحوليها
+
+388
+00:28:48,690 --> 00:28:54,790
+ل NO2، بعد هيك بتحوله لناترات، ناترات هذا كمان في
+
+389
+00:28:54,790 --> 00:28:58,570
+عند بكتيرا محللة للنيتروجين من الناترات، بتوصله
+
+390
+00:28:58,570 --> 00:29:02,830
+للجو، و أيضا كمان في عندنا نباتات بتستطيع تحطم هذا
+
+391
+00:29:02,830 --> 00:29:06,930
+الناترات، و هيك بيكون خلصت أنا الموضوع لليوم هذا
+
+392
+00:29:08,520 --> 00:29:12,680
+الشارع هنفصل عنه و أنا الطالبة همسة طافش شكرا لكم
+
+393
+00:29:15,290 --> 00:29:18,470
+السلام عليكم، اليوم موضوعنا عن القتش بالوري هي
+
+394
+00:29:18,470 --> 00:29:23,130
+المتواجد من المعدة البكتيرية، هذه معناه اللي من
+
+395
+00:29:23,130 --> 00:29:27,810
+نوع ال helobacter، helo معناها حلزوني، bacter،
+
+396
+00:29:27,810 --> 00:29:31,510
+بكتيريا، بالوري معناها اللي هي بتوجد في البوابة ما
+
+397
+00:29:31,510 --> 00:29:36,710
+بين المعدة والاثنين عشر تم اكتشافها قديمًا في 1875
+
+398
+00:29:36,710 --> 00:29:41,730
+على اعتماد ان هي بكتيريا تعيش في المعدة بسبب وجود
+
+399
+00:29:41,730 --> 00:29:47,600
+أنزيمات وأحماض في المعدةولكن بعد ذلك في 1879 تم
+
+400
+00:29:47,600 --> 00:29:52,480
+عزلها فعلا في المختبر وفي الف .. وفي 2005 حصلوا
+
+401
+00:29:52,480 --> 00:29:57,100
+على جائزة نوبل لعزل هذه البكتيريا هذه البكتيريا
+
+402
+00:29:57,100 --> 00:30:01,840
+بتعيش في المعدة لعدة أسباب بتمكنها أو لظروف مناسبة
+
+403
+00:30:01,840 --> 00:30:08,140
+لها منها أنه في عندها أنزيمات بتعيش في الغشاء
+
+404
+00:30:08,140 --> 00:30:13,280
+المبطن اللي يجدع المعدةفبتكون مختفية نوعا ما بها
+
+405
+00:30:13,280 --> 00:30:17,340
+السبب التانى ان عندها بتفرز أنزيم الورياز هذا
+
+406
+00:30:17,340 --> 00:30:21,060
+بيعمل على تحويل إفرازات المعدة إلى أمونيا الأمونيا
+
+407
+00:30:21,060 --> 00:30:24,560
+طبعا قلوية فبتعد الحمض المعدة بالتالي بتساعدها
+
+408
+00:30:24,560 --> 00:30:28,740
+العيش التانى السبب التالت أن ممكن الأغذية اللى
+
+409
+00:30:28,740 --> 00:30:32,400
+بتكون تحتوى على نسبة كبيرة من السكريات والأملح
+
+410
+00:30:32,400 --> 00:30:37,540
+والدهون بتساعد تكثرها بشتر طرق انتقالها
+
+411
+00:30:40,830 --> 00:30:44,010
+طرق انتباه طبعا عن ال contamination ال food و ال
+
+412
+00:30:44,010 --> 00:30:50,730
+water و ممكن كمان من person to person في نوع منها
+
+413
+00:30:50,730 --> 00:30:54,150
+بينتقل عن طريق النوع الخطط و الكلاب طبعا هاي نص
+
+414
+00:30:54,150 --> 00:30:59,930
+السكان العالم بصاب بهذه ال bacteria ال bacteria
+
+415
+00:30:59,930 --> 00:31:05,870
+هذه لها كذا أعراض من هذه الأعراض اللي ممكن تكونغثا
+
+416
+00:31:05,870 --> 00:31:11,830
+يعني، ممكن تقيق، بُقدن بالوزن، ممكن شعور بالجوع
+
+417
+00:31:11,830 --> 00:31:16,790
+بعد الأكل، ولكن هذه الأعراض مش .. مش كل الناس نفس
+
+418
+00:31:16,790 --> 00:31:20,170
+الأعراض، ممكن تكون خفيفة، ممكن تكون هذه الأعراض
+
+419
+00:31:20,170 --> 00:31:25,130
+بالظبط متواجدة عندهم، وجودة إن حوالي 85% من
+
+420
+00:31:25,130 --> 00:31:29,050
+الأعراض، من الأشخاص المصابين بقرحت المعدة بتكون
+
+421
+00:31:29,050 --> 00:31:34,120
+عندهم متواجدة البكتيريا الحلزونيةو فيها ال 20 ال
+
+422
+00:31:34,120 --> 00:31:39,340
+30 سنة عندهم بتكون معرضين للاصابة بأوراق المعدة
+
+423
+00:31:39,340 --> 00:31:44,940
+بهذه البكتيريا و فيه أيضا من الأوراق الشائعة اللي
+
+424
+00:31:44,940 --> 00:31:51,020
+هي مرض شلل الرعاش وجدوا أنه هذه البكتيريا لها سبب
+
+425
+00:31:51,020 --> 00:31:54,820
+رئيسي في هذا المرض لإنه لو ييجوا يحلله ممكن تكون
+
+426
+00:31:54,820 --> 00:31:58,580
+عندهم positive result في هذه البكتيريا لذلك يجب أن
+
+427
+00:31:58,580 --> 00:32:05,110
+نعالجها حتى تختفي هذا المرض شلل الرعاشكيف بتقدر
+
+428
+00:32:05,110 --> 00:32:08,670
+بكتير شرد الرعاش؟ ان البكتيريا بتفرز مادة شبيه
+
+429
+00:32:08,670 --> 00:32:13,990
+بالكلسترول تسمى modified cholesterol هذه مادة سامة
+
+430
+00:32:13,990 --> 00:32:17,090
+للجهاز العصبي و بتسبب هذا المرض
+
+431
+00:32:19,930 --> 00:32:24,610
+كمان إلها دور مهم في موضوع الانيميا بتعمل على نقص
+
+432
+00:32:24,610 --> 00:32:29,310
+امتصاص الحديد في الرسم لإن هي أصلا تحتاج الحديد في
+
+433
+00:32:29,310 --> 00:32:33,930
+تكاثرها كمان في أنواع من الانيميا الخفيفة اسمها
+
+434
+00:32:33,930 --> 00:32:37,950
+pernicious anemia هاي بتسبب له autoimmune
+
+435
+00:32:37,950 --> 00:32:42,490
+gastritis بتعمل اتهار في المعدة بتعمل على منع
+
+436
+00:32:42,490 --> 00:32:47,510
+امتصاص ال vitamin B12 كيف هنشخص هاي البكتيريا؟
+
+437
+00:32:48,520 --> 00:32:56,300
+أولاشى فين عن طريق اللى هو blood store فيننا blood
+
+438
+00:32:56,300 --> 00:33:01,620
+test blood test هنبحث عن ال antibodies للعينة إذا
+
+439
+00:33:01,620 --> 00:33:06,040
+كان موجود عيننا ال IGM إذا هتكون positive لهذه
+
+440
+00:33:06,040 --> 00:33:10,460
+البكتيريا ال IGG هنستخدمها لو كان في عيننا كت ممكن
+
+441
+00:33:10,460 --> 00:33:16,000
+نستخدم اللى بتتبين عيننا اللى IGGولكن إنه هنا أن
+
+442
+00:33:16,000 --> 00:33:19,860
+ال antibody بتبقى شهور و سنين موجودة في العينة
+
+443
+00:33:19,860 --> 00:33:23,940
+ممكن تعطي positive result حتى بعد العلاج ولكن ممكن
+
+444
+00:33:23,940 --> 00:33:28,900
+يستخدم أو أفضل استخدام التحليل اللي هو stool test
+
+445
+00:33:28,900 --> 00:33:33,660
+stool test ممكن يعطي اللي هو false negative حتى
+
+446
+00:33:33,660 --> 00:33:39,470
+بعد ما ينتهي العلاجفي كمان عن طريق اللي هو المنظر
+
+447
+00:33:39,470 --> 00:33:44,010
+هناخد عينة من جزء المصاب من الماعدة و بنحللها
+
+448
+00:33:44,010 --> 00:33:47,790
+لنشوف .. نكتشف مادة اليورياز إذا اليورياز موجود
+
+449
+00:33:47,790 --> 00:33:54,110
+إذا البكتيريا موجودة و بتكون ناشطة في هنا ال brave
+
+450
+00:33:54,110 --> 00:33:59,870
+test هذا عبارة إنه إن الإنسان ممرض بينفخ في كيس
+
+451
+00:34:00,250 --> 00:34:03,370
+هناخد العينة اللى نفق فيها بالكيس هنحطها على
+
+452
+00:34:03,370 --> 00:34:06,910
+الكربونا والكربونا هنحطها على الجهاز فهنقيص
+
+453
+00:34:06,910 --> 00:34:11,670
+ونقرأها نقرأ اللى هل موجودة عنده مثلا البكتيريا او
+
+454
+00:34:11,670 --> 00:34:17,070
+لا هنا هذه ممتازة جدا ولكن مكلفة جدا جدا هادى طيقة
+
+455
+00:34:17,070 --> 00:34:20,570
+ويفضل قاعدة الفحص عند أخذ العلاج من خلال هو أربع
+
+456
+00:34:20,570 --> 00:34:25,720
+لست أسابيعطرق العلاج فيه هنا أنه بالرغم من كل
+
+457
+00:34:25,720 --> 00:34:29,340
+سلبيات هذه البكتيريا ولكن أنه البعض بفضل أنه
+
+458
+00:34:29,340 --> 00:34:33,400
+مانعالجهاش إذا ماكانش فيه هنا مشاكل لإنه هي بتسبب
+
+459
+00:34:33,400 --> 00:34:38,710
+في معالج الالتهابات أو القلونتقليل من تهيبات
+
+460
+00:34:38,710 --> 00:34:43,310
+القلوب ولكن لو احنا بدنا نيجي نعالجها حاول الكثير
+
+461
+00:34:43,310 --> 00:34:47,170
+من علماء اكتشاف vaccine ضد هذه البكتيريا ولكن هم
+
+462
+00:34:47,170 --> 00:34:50,870
+يستطيعوا بسبب وجود أرماء كثيرة من هذه البكتيريا
+
+463
+00:34:50,870 --> 00:34:56,030
+فهنا في الآخر انه اكتشفوا triple therapy مناد حيوي
+
+464
+00:34:56,030 --> 00:35:01,670
+عبارة بيحتيبوا على ال .. ال omibrasol وفيه عندنا
+
+465
+00:35:01,670 --> 00:35:04,830
+ال omoxycylene هدول هيكون ال antitherapy ضد
+
+466
+00:35:04,830 --> 00:35:09,900
+البكتيرياالسلام عليكم ورحمة الله وبركاته انا
+
+467
+00:35:09,900 --> 00:35:13,300
+الطالب هاي بخلص بدي اتحدث عن موضوع ال probiotic
+
+468
+00:35:13,300 --> 00:35:20,480
+بشكل مختصر what are probiotic? probiotic are live
+
+469
+00:35:20,480 --> 00:35:24,380
+microorganism in most cases bacteria and yeast
+
+470
+00:35:24,380 --> 00:35:27,880
+that are similar to beneficial microorganism found
+
+471
+00:35:27,880 --> 00:35:34,820
+in human gut اذا probiotic هي عبارة عن كئنات حية
+
+472
+00:35:34,820 --> 00:35:42,910
+دقيقةأغلب الأحيان بكتيريا و خمائة موجودة في أمعاء
+
+473
+00:35:42,910 --> 00:35:45,590
+الإنسان بشكل طبيعي تقريبا زي ال normal في الأوراق
+
+474
+00:35:45,590 --> 00:35:50,530
+نقدر نسميها اسم تاني اللي هي ال friendly bacteria
+
+475
+00:35:50,530 --> 00:35:54,650
+و ال good bacteria يعني ما بتسببنا pathogen أمراض
+
+476
+00:35:54,650 --> 00:35:59,310
+و موجودة في ال dietary supplement اللي هي
+
+477
+00:35:59,310 --> 00:36:02,370
+المكاملات الغذائية و ال food في العديد من الأوقات
+
+478
+00:36:02,370 --> 00:36:09,770
+عامة بنلاقيهhow do probiotic work؟ كيف بيشتغل ال
+
+479
+00:36:09,770 --> 00:36:15,910
+probiotic في الجسم؟ probiotic
+
+480
+00:36:15,910 --> 00:36:20,010
+can improve intestinal function and maintain the
+
+481
+00:36:20,010 --> 00:36:24,330
+integrity of the lining of the intestine اللي هو
+
+482
+00:36:24,330 --> 00:36:31,720
+بيساعد .. بيحملبيحمل بطانة الداخلية للأمعاق و كمان
+
+483
+00:36:31,720 --> 00:36:37,040
+بيسهل حركة الطعام أثناء الأمعاق بحيث أنه بيمنع أنه
+
+484
+00:36:37,040 --> 00:36:40,280
+يمشي بشكل سريع يسبب دياريا أو بشكل بطيء يسبب
+
+485
+00:36:40,280 --> 00:36:44,500
+quanticitation Probiotic
+
+486
+00:36:44,500 --> 00:36:47,780
+may help lots of ailments يعني ممكن تحب العديد من
+
+487
+00:36:47,780 --> 00:36:52,400
+الأمراض عنا treating childhood diarrhea treating
+
+488
+00:36:52,400 --> 00:36:58,260
+ulcerative colitis اللي هي ال ..قلان القراحي
+
+489
+00:36:58,260 --> 00:37:04,380
+treating necrotizing enterocolitis هذه الاكتهابات
+
+490
+00:37:04,380 --> 00:37:07,820
+بتنتج عند الأطفال حديثي الولادة ممكن تسبب للوفاة
+
+491
+00:37:07,820 --> 00:37:13,160
+prevent antibiotic أساسيات الديارية and infectious
+
+492
+00:37:13,160 --> 00:37:20,760
+ديارية في بعض ال antibiotic ممكن ينتج عنها ديارية
+
+493
+00:37:20,760 --> 00:37:25,560
+ال property بتمنع هذا الأشياء زي ال clindamycin
+
+494
+00:37:26,550 --> 00:37:30,090
+Preventing Pauschitis إنها الإتهابات اللي بتنتج عن
+
+495
+00:37:30,090 --> 00:37:34,450
+عمليات جراحية للأنعاق Treating and Preventing
+
+496
+00:37:34,450 --> 00:37:39,130
+Eczema أساسات الوثكاوز ميت ألرجيه في حسية بتنتج عن
+
+497
+00:37:39,130 --> 00:37:43,810
+بعض اللي عن شرب حليب البقر البروبا يتكيب تمنعها
+
+498
+00:37:43,810 --> 00:37:50,470
+Helping the immune system طبعا هنا كيف البروبا
+
+499
+00:37:50,470 --> 00:37:55,650
+يتكيب تعمل أهمش إنها help in gut health يعني حفاظ
+
+500
+00:37:55,650 --> 00:38:02,050
+على الأنعاقهذا مثال lactobacillus مثال على نوع من
+
+501
+00:38:02,050 --> 00:38:05,650
+أنواع البكتيريا اللى برو بايتك موجودة في ال
+
+502
+00:38:05,650 --> 00:38:10,730
+digestive system ال urinal الgenital system طبعا
+
+503
+00:38:10,730 --> 00:38:14,690
+بشكل طبيعي مابت سبب أمرض إنه في العديد من ال
+
+504
+00:38:14,690 --> 00:38:17,870
+species كتير منها أهمها اللى lactobacillus
+
+505
+00:38:17,870 --> 00:38:23,430
+acidophilus أشهر الأنواع ال most commonly used in
+
+506
+00:38:23,430 --> 00:38:26,470
+probiotic اللى friendly bacteria
+
+507
+00:38:28,850 --> 00:38:33,130
+طبعا بما انه بس .. بس يعني عصوية هيه لك تبصيه بس
+
+508
+00:38:33,130 --> 00:38:40,050
+كنت بشربها على شكل قدر مصادر ممكن نحصل من خلالها
+
+509
+00:38:40,050 --> 00:38:45,050
+على ال probiotic بنلاقيها في ال yogurt اللي هو
+
+510
+00:38:45,050 --> 00:38:51,270
+الحريب، الملك، اللبن يعني، الملك، الاديون،
+
+511
+00:38:51,270 --> 00:38:55,110
+التواتس، الويت، اللامت، ال .. ال honey
+
+512
+00:38:59,530 --> 00:39:03,410
+available form كيف ممكن نبنعه لل .. ل ال
+
+513
+00:39:03,410 --> 00:39:06,510
+probiotic؟ ممكن تكون على شكل freeze جان ..
+
+514
+00:39:06,510 --> 00:39:10,290
+granularي على حبيبات أو powder، capsule ممكن تكون
+
+515
+00:39:10,290 --> 00:39:15,530
+ساقة، liquid أو ممكن تكون على شكل tablets، أكراس
+
+516
+00:39:15,530 --> 00:39:16,830
+أو للتحرير
+
+517
+00:39:21,410 --> 00:39:25,110
+بسم الله الرحمن الرحيم السلام عليكم الموضوع اليوم
+
+518
+00:39:25,110 --> 00:39:29,450
+بيربط ما بين ال E.coli وما بين الملاريا، هلأ كيف
+
+519
+00:39:29,450 --> 00:39:32,130
+..عارفوا بتعرفوا، بالنسبة اللي راح ناخد نفسي صغيرة
+
+520
+00:39:32,130 --> 00:39:35,450
+عن مرض الملاريا، راح ناخد نفسي صغيرة عن مرض
+
+521
+00:39:35,450 --> 00:39:39,110
+الملاريا، بالنسبة لمرض الملاريا هو من الأمراض ..هو
+
+522
+00:39:39,110 --> 00:39:43,680
+مرض بسبب برساية اسمه ال plasmodiumهذا المرض بيكون
+
+523
+00:39:43,680 --> 00:39:47,160
+ال vector لإله أوه بينتقل من الإنسان لإنسان عن
+
+524
+00:39:47,160 --> 00:39:53,200
+طريق بعوضة ال enophils شائع أو منتشر هذا المرض في
+
+525
+00:39:53,200 --> 00:39:56,100
+مناطق .. في مناطق هضول الأمطار، المناطق اللي بيكتب
+
+526
+00:39:56,100 --> 00:40:00,180
+فيها المستنقعات و خاصة الدول الأفريقية، الأفريقية،
+
+527
+00:40:00,180 --> 00:40:07,920
+في عام 2012 اتسجلت حوالي 627 ألف حالة وفاة نتيجة
+
+528
+00:40:07,920 --> 00:40:12,160
+للإصابة بالملاريا، 90% منهم كان من الأطفالركزوا
+
+529
+00:40:12,160 --> 00:40:14,940
+على هذه النقطة اللي احنا بيعلموا، حلو بالنسبالي
+
+530
+00:40:14,940 --> 00:40:19,000
+دورة ال .. دورة ال .. دورة ال plasmodium أو دورة
+
+531
+00:40:19,000 --> 00:40:22,840
+.. دورة دورة المرض مش كتير، راح نتعمق فيها، لكن
+
+532
+00:40:22,840 --> 00:40:25,540
+فيه هنا بعوضة، هاي البعوضة بتحتوي على ال
+
+533
+00:40:25,540 --> 00:40:29,920
+plasmodium ب .. فيه قلوب دورته two cycle، دورة
+
+534
+00:40:29,920 --> 00:40:33,760
+بيحصل في البعوضة و دورة في الإنسان، حلو بيقعد في
+
+535
+00:40:33,760 --> 00:40:37,800
+البعوضة حوالي سبوع، بعدين بينتقل يصل لعند ال ..
+
+536
+00:40:37,800 --> 00:40:41,400
+الغدد اللي وعديه لإلها، لما تعمل بيت لإنسان سليم
+
+537
+00:40:41,730 --> 00:40:46,470
+بديها تحقنه في ال parasite هلأ وصل ال parasite ال
+
+538
+00:40:46,470 --> 00:40:51,270
+target الأول لإله رح يكون في ال liver طبعا في ال
+
+539
+00:40:51,270 --> 00:40:55,250
+liver بيضلوا حوالي أسبوع على ما هو بيستهدف ال RBCs
+
+540
+00:40:55,250 --> 00:40:59,990
+بيضلوا فيها حوالي من يومين ل 3 أيام بعدين بسبب
+
+541
+00:40:59,990 --> 00:41:03,990
+لإلها lysis بتطلع عندك الميه من ال parasite
+
+542
+00:41:03,990 --> 00:41:09,150
+وبالتالي بتنتشر في الجسم طبعا بتكون systemic لإن
+
+543
+00:41:09,150 --> 00:41:13,270
+الدم موجود وين ما كانبعدين لو إجت .. لو إجت بعض
+
+544
+00:41:13,270 --> 00:41:17,990
+تانية و راحت .. أخدت من ذنب هذا المصاب طبعا و
+
+545
+00:41:17,990 --> 00:41:25,230
+أحققته أو عملت بثلة إن إنسان سليم أخدت انتقل لشخص
+
+546
+00:41:25,230 --> 00:41:29,490
+تاني، هلأ أنا قولتلكوا إن الموضوع بيربط ما بين
+
+547
+00:41:29,490 --> 00:41:32,310
+المرارية و ما بين ال E.coli، بالنسبة لل E.coli
+
+548
+00:41:32,310 --> 00:41:36,350
+طبعا هي غنية التعريف، ال E.coli فيه حوالي أكتر من
+
+549
+00:41:36,350 --> 00:41:41,930
+700 سيرو تايب من ال E.coliعدد قليل منها ممكن يسبب
+
+550
+00:41:41,930 --> 00:41:44,830
+infection للإنسان وطبعا بتعرف ال urine .. ال
+
+551
+00:41:44,830 --> 00:41:47,290
+urinary tract infection يعني angiasis ال
+
+552
+00:41:47,290 --> 00:41:50,390
+gastrointestinal tract infection ورفته ال
+
+553
+00:41:50,390 --> 00:41:52,710
+enteropathogenic E coli و ال enterohemorrhage كل
+
+554
+00:41:52,710 --> 00:42:00,450
+هاي أنموعة بتسبب infection للإنسان بالنسبة لغالبية
+
+555
+00:42:00,450 --> 00:42:03,370
+الأظمة من هاي ال 700 سيروتاير طبعا نفسهم بناء على
+
+556
+00:42:03,370 --> 00:42:06,550
+ال A و ال K و ال H انتجين هي عبارة عن نور مالفلورا
+
+557
+00:42:06,550 --> 00:42:13,110
+موجودة في ال gastrointestinal tractطيب، هلأ طبعا
+
+558
+00:42:13,110 --> 00:42:17,070
+مرمعكوا أنه غير فوائد ال normal flora أو بالتحديد
+
+559
+00:42:17,070 --> 00:42:21,110
+ال E.coli أنه هي غير أنها بتعمل مقاومة للاستعمار ب
+
+560
+00:42:21,110 --> 00:42:26,750
+infected microorganism أخرى، أيضا هي بتعمل على
+
+561
+00:42:26,750 --> 00:42:30,670
+إنتاج vitamin Kكمان بتعمل على .. بتساعد على
+
+562
+00:42:30,670 --> 00:42:35,510
+الانتصاص اليوم اكتشفوا وظيفة جديدة او الدراسة
+
+563
+00:42:35,510 --> 00:42:39,070
+اليوم بتحكي انه في انها وظيفة جديدة تم اكتشافها لل
+
+564
+00:42:39,070 --> 00:42:42,630
+E.coli او بعض الأسترال من ال E.coli الموجودة ك ..
+
+565
+00:42:42,630 --> 00:42:45,970
+كnormal flora في ال gastrointestinal tract هلأ
+
+566
+00:42:45,970 --> 00:42:50,330
+الدراسة بتقول انه في ان بعض الأسترال من ال E.coli
+
+567
+00:42:50,330 --> 00:42:56,410
+عندها القدرة على مقاومة مرض الملاريا كيف؟ هلأ ال E
+
+568
+00:42:56,410 --> 00:43:00,630
+.coliبعد ال strength طبعا من ال equalها بتقدر تنتج
+
+569
+00:43:00,630 --> 00:43:04,990
+sugar او glycine هذا ال glycine بتروح طبعا يحفظ
+
+570
+00:43:04,990 --> 00:43:09,250
+جهاز المنع انه ينتج antibody ضده طيب جهاز المنع
+
+571
+00:43:09,250 --> 00:43:12,330
+انتج antibody ضده ايش علاقته بالملاريا؟ الملاريا
+
+572
+00:43:12,330 --> 00:43:16,830
+وجدوا انها بتقدر تنتج نفس هذا ال sugar اللي هو
+
+573
+00:43:16,830 --> 00:43:21,230
+alpha galactosidaseبتعمل exploration لإله، بالتالي
+
+574
+00:43:21,230 --> 00:43:24,550
+يعني هلأ لما يكون فيه عنا .. عنا نور E.coli موجود
+
+575
+00:43:24,550 --> 00:43:28,530
+.. أنتجت .. ساعدت جهاز المنع أنه ينتج Antibody،
+
+576
+00:43:28,530 --> 00:43:31,070
+ماشي، هلأ لما يصير فيه عنا .. فيه عنا كمية كافية
+
+577
+00:43:31,070 --> 00:43:33,150
+من ال Antibody، نفترض أنه فيه عنا كمية كافية من ال
+
+578
+00:43:33,150 --> 00:43:36,030
+Antibody، لما يصير فيه عنا infection بالملاريا،
+
+579
+00:43:36,030 --> 00:43:39,170
+بده، فهي ال Antibody اللي هي بالاصل مش تم تحفيزها
+
+580
+00:43:39,170 --> 00:43:41,730
+من ال .. من الملاريا اللي إجت، لأ من السابق كان
+
+581
+00:43:41,730 --> 00:43:46,250
+فيه عنا Antibody من ال .. من ال E.coli، بدها تروح
+
+582
+00:43:46,250 --> 00:43:50,210
+يتهاجم الملارياطيب، أنا قولتلكوا رجزة و نقطة هي
+
+583
+00:43:50,210 --> 00:43:53,670
+أنه 90% من حالات البرفالة تسجلت كانت بموضوعها من
+
+584
+00:43:53,670 --> 00:43:56,310
+الأطفال، طب ليش؟ و بتهيألي أنها هي النقطة اللي
+
+585
+00:43:56,310 --> 00:44:00,230
+لفتت انتباهي العلماء أنهم يبحثوا في هات موضوع، هلأ
+
+586
+00:44:00,230 --> 00:44:04,890
+وجدوا أنه الأطفال عندهم أو غير قادرين أو كمية ال
+
+587
+00:44:04,890 --> 00:44:08,030
+antibody اللي بتتم إنتاجها بوسطة هذا ال normal
+
+588
+00:44:08,030 --> 00:44:11,010
+flora كمية قليلة جدا غير قادرة على مقاومة علم اللي
+
+589
+00:44:11,010 --> 00:44:14,330
+عليهالا هيك بنلاقي ان معظم حالات البفار بيكون من
+
+590
+00:44:14,330 --> 00:44:17,950
+الأطفال و غير انه فينا people أصلا بيكون عندهم
+
+591
+00:44:17,950 --> 00:44:20,990
+مثلا جهاز المناعة ضعيف، هاي حالات البفار بتتسجل
+
+592
+00:44:20,990 --> 00:44:24,790
+بالقدر، فينا people بيكون عندهم مثلا جهاز المناعة
+
+593
+00:44:24,790 --> 00:44:28,530
+ضعيف، انه بالنهاية مافيش كمية كافية من ال anti
+
+594
+00:44:28,530 --> 00:44:33,140
+-alpha galactosidase antibodyطيب طبعا بدهم وظفوا
+
+595
+00:44:33,140 --> 00:44:37,720
+هذا ال .. هذه الدراسة باشي طبعا باندر فاكسين فعلا
+
+596
+00:44:37,720 --> 00:44:41,640
+قدروا .. راحوا جابوا اندر .. قدروا يعملوا فاكسين
+
+597
+00:44:41,640 --> 00:44:46,340
+طبعا احنا بنحكي عن فاكسين يعني بيكون جزء من ال ..
+
+598
+00:44:46,340 --> 00:44:48,840
+من البكتيريا او من البكتيريا .. او البكتيريا كلها
+
+599
+00:44:48,840 --> 00:44:52,160
+فإنه راحوا حقانهم و جربوا على فران .. على الفران
+
+600
+00:44:52,160 --> 00:44:56,680
+اللي هي nice test .. test و بالفعل تمقدرت الفلان
+
+601
+00:44:56,680 --> 00:45:01,100
+انها تقاوم مرض الملاريا وحاليا بتتم التطوير
+
+602
+00:45:01,100 --> 00:45:04,360
+بالاستفادة من هذه النقطة انهم يطوروا vaccine لمرض
+
+603
+00:45:04,360 --> 00:45:10,980
+الملاريا مثلا انهإنهم يعملوا كجزء من جدول التطقيم
+
+604
+00:45:10,980 --> 00:45:14,280
+للأشخاص .. للمناطق الموبوعة بالملاريا، هذا شغله،
+
+605
+00:45:14,280 --> 00:45:17,640
+شغلة تانية ممكن الأشخاص المسافرين اللي بدهم
+
+606
+00:45:17,640 --> 00:45:22,480
+ينتقلوا لمناطق الموبوعة بالملاريا، إنهم ياخدوا هذا
+
+607
+00:45:22,480 --> 00:45:27,600
+ال vaccine، خد بس، نقطة أخيرة بس بتعزز المفهوم إنه
+
+608
+00:45:27,600 --> 00:45:32,500
+إحنا عارفين إنه ال normal flora الموجودة في
+
+609
+00:45:32,500 --> 00:45:37,550
+أجسامنا بتختلف منبتختلف في سكان المناطق .. بتختلف
+
+610
+00:45:37,550 --> 00:45:41,150
+من منطقة لأخرى في أجسام يعني سكان منطقة سواء في ال
+
+611
+00:45:41,150 --> 00:45:44,950
+.. سواء في الأنواع تبعتها أو في ال rate تبعي ..
+
+612
+00:45:44,950 --> 00:45:48,670
+المعدل تبعها بتختلف فهن وجدوا أنه الناس اللي
+
+613
+00:45:48,670 --> 00:45:51,670
+بيسافروا لمناطق الموبوعة بالmalaria عندهم
+
+614
+00:45:51,670 --> 00:45:54,590
+susceptibility للإصابة بالmalaria أكتر من الناس
+
+615
+00:45:54,590 --> 00:45:58,850
+اللي عايشين في .. مقيمين في نفس ال .. المنطقة
+
+616
+00:45:58,850 --> 00:46:02,620
+فيعني هاي ال normal flora خلينا نقول أنهيعني أحد
+
+617
+00:46:02,620 --> 00:46:06,160
+الأسباب أنه هاي ال normal people موجودة بأجسامهم
+
+618
+00:46:06,160 --> 00:46:09,800
+في ال gastro intestinal tract كمية أكبر أو .. طيب
+
+619
+00:46:09,800 --> 00:46:15,460
+سبحان الله يعني .. هاي ربنا أعطاهم شغل أنهم متكيف
+
+620
+00:46:15,460 --> 00:46:20,040
+مع البيئة اللي عايشة الحقيقة
+
+621
+00:46:20,040 --> 00:46:24,320
+أنه بدي أتقدم باليدير الشكر للثلاثة اللي سبقوا
+
+622
+00:46:24,320 --> 00:46:27,380
+السبت الماضي في ال presentation و للأربع اللي هذا
+
+623
+00:46:27,380 --> 00:46:31,310
+أيضا اللي اليوم تقدموه باربع مواضيعاليستهيله
+
+624
+00:46:31,310 --> 00:46:35,090
+بكتيريا والـ H-Pyri و الـ Probiotics والمياري Anti
+
+625
+00:46:35,090 --> 00:46:40,430
+-coli كل المواضيع فيها معلومات جديدة وشيقة ويمكن
+
+626
+00:46:40,430 --> 00:46:44,510
+ترجمتها إلى مقالات تُكتب على مجلة قوية علم الصحية
+
+627
+00:46:44,510 --> 00:46:49,050
+إن شاء اللهلكن بدها جزئيات يعني اشتهدت فيها يعني
+
+628
+00:46:49,050 --> 00:46:53,790
+حضرتك H-Pylori مع الشهرة للراعاش هذه الجزئية هي
+
+629
+00:46:53,790 --> 00:46:57,510
+اللي انا اكتف فيها ال probiotic بدنا .. بدنا حاجة
+
+630
+00:46:57,510 --> 00:47:00,210
+جديدة ماشوفش حاجة جديدة انا بالنسبة لي بدنا حاجة
+
+631
+00:47:00,210 --> 00:47:03,030
+جديدة ال malaria والإيشي إيشي كلها ال science
+
+632
+00:47:03,030 --> 00:47:08,330
+حاجات كتيرة محاضرة متنوعة عملها انا عامة عن فوائد
+
+633
+00:47:08,330 --> 00:47:11,370
+ال normal flora بس حقيقة احد الفوائد بالفعل ان
+
+634
+00:47:11,370 --> 00:47:12,930
+they teach the immune system
+
+635
+00:47:18,880 --> 00:47:22,820
+بالضبط كمان نقطة ايه؟ نقطة مهم جدا إذا قلتلكم إن
+
+636
+00:47:22,820 --> 00:47:26,240
+number of normal flora هو تقريبا
+
+637
+00:47:30,370 --> 00:47:33,570
+ف you are more micro than a human يعني أنتم
+
+638
+00:47:33,570 --> 00:47:38,050
+ميكروبات أكتر منكم يعني طيب لو قلتلكوا أنه عدد
+
+639
+00:47:38,050 --> 00:47:42,810
+الجينات و ال contribution 150 ل 1 يعني كلمة عدد
+
+640
+00:47:42,810 --> 00:47:45,150
+الجينات أقصد فيها أنواع الجينات اللي مولة في
+
+641
+00:47:45,150 --> 00:47:49,990
+الميكروبات تنوعة 150 ل 1 فالجينات برضه اللي بتنتج
+
+642
+00:47:49,990 --> 00:47:52,870
+من قبل هذه البكتيريا أكيد بتنتج أشياء مفيدة احنا
+
+643
+00:47:52,870 --> 00:47:58,230
+مش عارفينها بس احنا للأسفالبشر إذا بيعرفوا معلومة
+
+644
+00:47:58,230 --> 00:48:02,530
+بيستعدلوا في قطاف النتائج عرفنا ان البكتيريا مؤدر
+
+645
+00:48:02,530 --> 00:48:05,770
+الإنسان kill them all اقتل كل الخلايا اقتل كل
+
+646
+00:48:05,770 --> 00:48:10,870
+البكتيريا دمرها وبالتالي في الآخر قتلناها وبعد ما
+
+647
+00:48:10,870 --> 00:48:13,810
+قتلناها برا تظهر لنا أمراض أخرى لما كنا عارفها من
+
+648
+00:48:13,810 --> 00:48:16,950
+قبل نتجي عن خلال ال normal flower لحقيقة المعلومات
+
+649
+00:48:16,950 --> 00:48:20,790
+كلها جيدة الإلقاء كمان كان جيد من قبل الجميع سيانو
+
+650
+00:48:20,790 --> 00:48:24,550
+بكتيريا يعني يالا تكتب عن استخداماتهاعلى استخدامات
+
+651
+00:48:24,550 --> 00:48:27,470
+ال cyanobacteria لإنه برضه لها .. كنتوا عارفوا إنه
+
+652
+00:48:27,470 --> 00:48:29,730
+زمان كانوا يقولولنا نباتات مهمة على كوكب الأرض
+
+653
+00:48:29,730 --> 00:48:34,410
+لتنتج oxygen نص ال oxygen أو أكتر من نصه اللي
+
+654
+00:48:34,410 --> 00:48:37,490
+بينتج على كوكب الأرض هو من ال cyanobacteria
+
+655
+00:48:37,490 --> 00:48:42,770
+وبالتالي احنا ندين لهذه ال bacteria بيش يعني
+
+656
+00:48:42,770 --> 00:48:46,550
+ندينها بكثير ندينها بالكثير طيب احنا حلصنا ال
+
+657
+00:48:46,550 --> 00:48:48,590
+presentation وقت انتهى فان شاء الله بيشمل مرة
+
+658
+00:48:48,590 --> 00:48:49,230
+القادرة بإننا
+
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/UgPzLIzLs8w.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/UgPzLIzLs8w.srt
new file mode 100644
index 0000000000000000000000000000000000000000..d7ce9491ccfc18432b9e31d8df5bd2c5eab9bfdf
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/UgPzLIzLs8w.srt
@@ -0,0 +1,2195 @@
+1
+00:00:20,540 --> 00:00:22,360
+أعوذ بالله، السلام عليكم، المشي طالع جيد، بسم الله
+
+2
+00:00:22,360 --> 00:00:26,260
+الرحمن الرحيم، نكمل الـ streptococci، توقفنا المرة 
+
+3
+00:00:26,260 --> 00:00:29,400
+الماضية عند نوع آخر من الـ classification لـ streptococci
+
+4
+00:00:29,400 --> 00:00:33,460
+اللي هو Lansfield grouping أو Lansfield 
+
+5
+00:00:33,460 --> 00:00:37,800
+classification، وعرفنا أنه Rebecca Lansfield
+
+6
+00:00:37,800 --> 00:00:40,920
+classified streptococci based on a surface
+
+7
+00:00:40,920 --> 00:00:45,640
+antigen into groups، كلمة group تعني أن one or more
+
+8
+00:00:45,640 --> 00:00:51,230
+species are included in that group، كان مرة based on
+
+9
+00:00:51,230 --> 00:00:55,530
+surface antigen، للأسف وجدنا أن هذا الـ surface
+
+10
+00:00:55,530 --> 00:00:59,810
+antigen موجود في بعض الـ streptococci، وبالتالي صنفناها
+
+11
+00:00:59,810 --> 00:01:03,190
+وسَميناها groupable، يعني قابلة للتصنيف أو قابلة 
+
+12
+00:01:03,190 --> 00:01:08,330
+للوضع في مجموعات مثل مجموعة A وB وD وهي الأهم على 
+
+13
+00:01:08,330 --> 00:01:12,130
+الإطلاق لـ streptococci، هناك مجموعات أقل أهمية لها
+
+14
+00:01:12,130 --> 00:01:16,270
+أهمية لكن أقل زي الـ C وG وF وهي نادرة أن تسبب
+
+15
+00:01:16,270 --> 00:01:20,940
+أمراض للإنسان، والـ non-groupable اللي هما كانوا
+
+16
+00:01:20,940 --> 00:01:25,140
+مثلًا streptococcus pneumoniae وطبعًا هي مشهورة كثير
+
+17
+00:01:25,140 --> 00:01:27,800
+يعني مش بقى أحكي أنها non-groupable لأنها أقل
+
+18
+00:01:27,800 --> 00:01:31,770
+أهمية، لا، أهميتها كبيرة، الـ viridans streptococci
+
+19
+00:01:31,770 --> 00:01:35,190
+اللي بتمثلها الـ streptococcus mutans اللي بتعمل الـ
+
+20
+00:01:35,190 --> 00:01:39,450
+dental caries، ومجموعة أخرى من أفرادها سميناها 
+
+21
+00:01:39,450 --> 00:01:42,810
+viridans group، ده بتتذكروا viridans group، مجموعة
+
+22
+00:01:42,810 --> 00:01:47,850
+الخضراء اللي قد أو خدنا نقول في evidence عالي جدًا
+
+23
+00:01:47,850 --> 00:01:52,390
+من الأبحاث بتقول أنها ممكن تسبب endocarditis
+
+24
+00:01:52,390 --> 00:01:56,710
+endocarditis، تمتلك مقدرة مهولة على الـ attachment
+
+25
+00:01:57,160 --> 00:02:00,660
+فبالتالي عندها المقدرة في القلب، تشوف القلب نفسه،
+
+26
+00:02:00,660 --> 00:02:04,320
+عدد القلب، تبقى هناك، وين في القلب؟ مضخة الدم، يعني 
+
+27
+00:02:04,320 --> 00:02:08,400
+في قوة شديدة من الضغط قد يدفع البكتيريا إلى
+
+28
+00:02:08,400 --> 00:02:11,860
+الخارج، لكن مقدرتها الـ attachment عالية كمان كده
+
+29
+00:02:11,860 --> 00:02:15,480
+زي
+
+30
+00:02:15,480 --> 00:02:18,220
+أي بكتيريا أخرى، في عهد الجينك تمتلك toxins و
+
+31
+00:02:18,220 --> 00:02:22,340
+enzymes، لكن نعود بكم إلى التصنيف الثاني أو
+
+32
+00:02:22,340 --> 00:02:26,200
+الأولاني، الأساسي اللي احنا أخذناه، اللي هو قلنا
+
+33
+00:02:26,200 --> 00:02:30,240
+إن الـ streptococci قد تُصنّف إما Alpha أو Beta أو
+
+34
+00:02:30,240 --> 00:02:33,060
+Gamma، والـ Alpha اللي هي الـ incomplete ..
+
+35
+00:02:33,060 --> 00:02:37,140
+incomplete partial hemolysis as indicated by
+
+36
+00:02:37,140 --> 00:02:40,280
+greenish discoloration، يعني التلون باللون الأخضر
+
+37
+00:02:40,280 --> 00:02:44,730
+حول المستعمرة، الـ Beta-Hemolysis complete as
+
+38
+00:02:44,730 --> 00:02:47,710
+indicated by complete clearance، يعني ما فيش أي لون
+
+39
+00:02:47,710 --> 00:02:51,230
+حول المستعمرة، الجامعة زي ما ذكرنا جاهزة ترضية
+
+40
+00:02:51,230 --> 00:02:54,930
+عطناها للبكتيريا non-hemolytic اللي لم تعمل 
+
+41
+00:02:54,930 --> 00:02:58,930
+Hemolysis، هذا الجدول مش لدى الحفظ، الجدول هذا فقط 
+
+42
+00:02:58,930 --> 00:03:02,190
+عشان يعطينا إيش وراء إن هناك different species
+
+43
+00:03:02,190 --> 00:03:06,490
+under different groups، الـ groups اللي هندرسها هي
+
+44
+00:03:06,490 --> 00:03:11,400
+اللي هنحفظها، أما مش الجدول هذا، الصورة اللي قدامنا
+
+45
+00:03:11,400 --> 00:03:17,260
+هذه صورة كثير معبرة، على الجهة اليسرى من اللوحة
+
+46
+00:03:17,260 --> 00:03:21,920
+مُبطّنة بنلاقي أسماء كثيرة لأمراض مثلًا meningitis,
+
+47
+00:03:23,100 --> 00:03:26,280
+otitis, sinusitis, tonsillitis, pharyngitis
+
+48
+00:03:26,280 --> 00:03:31,880
+عندنا adenitis, pneumonia, endocarditis, impetigo,
+
+49
+00:03:32,020 --> 00:03:35,620
+erysipelas, impetigo، مَرْمَعَانَة جابت هناك؟ آه، بتنتقل
+
+50
+00:03:35,620 --> 00:03:40,230
+.. وين مَرْمَعَانَة؟ philostepin O, staphylococcus aureus، هنا
+
+51
+00:03:40,230 --> 00:03:44,870
+قلنا نجا في لحظة ونتكلم عن مفهوم مولود في الـ
+
+52
+00:03:44,870 --> 00:03:49,870
+microbiology، two different organisms causes one
+
+53
+00:03:49,870 --> 00:03:53,630
+disease، هي MBT جوه، وهناك MBT جوه، نفس المسمى، نفس
+
+54
+00:03:53,630 --> 00:03:57,050
+الأعراض طبعًا، لكن لما نيجي نتكلم عن الـ treatment
+
+55
+00:03:57,050 --> 00:04:01,850
+لمرض واحد قد تسببه أكثر من الـ microbe، إيه بتكون
+
+56
+00:04:01,850 --> 00:04:06,360
+الحل عندنا؟ إنه لازم نعرف الميكروب المسبب للمرض حتى
+
+57
+00:04:06,360 --> 00:04:09,740
+نعطي specific treatment، الآن هذا أنا جلبت مثال
+
+58
+00:04:09,740 --> 00:04:14,120
+بسيط على two organism، two different organism cause
+
+59
+00:04:14,120 --> 00:04:18,160
+one similar disease، لكن جِئْتُ بمثال متطرف أكثر من 
+
+60
+00:04:18,160 --> 00:04:24,880
+هذا المثال، meningitis، خلال دراستنا السابقة و
+
+61
+00:04:24,880 --> 00:04:29,000
+اللاحقة، معظم الميكروبات اللي احنا ندرسها ممكن
+
+62
+00:04:29,000 --> 00:04:31,520
+تتسبب بـ meningitis، إذا الـ meningitis
+
+63
+00:04:34,540 --> 00:04:38,960
+types of bacteria، طيب لو قلنا أن it is caused by
+
+64
+00:04:38,960 --> 00:04:42,740
+bacteria، it is caused by parasites، مش بكتيريا
+
+65
+00:04:42,740 --> 00:04:46,940
+طفيليات، بروتوزوا، it may be caused by fungites
+
+66
+00:04:46,940 --> 00:04:50,640
+كريبتوكوكس نيوفورمانس، مشهور جدًا، الخميرة هذه بسبب
+
+67
+00:04:50,640 --> 00:04:54,620
+meningitis، viruses، viral meningitis، تسمع بالـ viral
+
+68
+00:04:54,620 --> 00:04:58,500
+meningitis؟ viral meningitis، يبقى فيروسات وبكتيريا
+
+69
+00:04:58,500 --> 00:05:01,260
+وطفيليات وفطريات
+
+70
+00:05:02,920 --> 00:05:07,200
+يعني الميكروبيولوجي كله بيعمل meningitis، طيب لما 
+
+71
+00:05:07,200 --> 00:05:11,780
+نُشَخِّص meningitis هذه، خطوة أن المريض has a
+
+72
+00:05:11,780 --> 00:05:15,880
+meningitis disease، لكن مين المسبب؟ هل هو viral؟ هل 
+
+73
+00:05:15,880 --> 00:05:18,200
+هو bacterial؟ وإيه ده bacterial؟ من نوع الـ
+
+74
+00:05:18,200 --> 00:05:21,960
+bacteria بالضبط؟ عشان نعطي specific treatment، وهنا
+
+75
+00:05:21,960 --> 00:05:25,620
+الدور العظيم اللي أنت بتقوم فيه كميكروبيولوجيين في
+
+76
+00:05:25,620 --> 00:05:29,140
+المختبرات، إنكم تحاولوا تعزلوا العامل المسبب
+
+77
+00:05:29,140 --> 00:05:34,560
+ويتعرفوا عليه، وأيضًا كمان تشوفوا مدى حساسيته أو
+
+78
+00:05:34,560 --> 00:05:37,480
+مقاومته للمضاد الحيوي، إذا الدور اللي منطلبكم بهذه
+
+79
+00:05:37,480 --> 00:05:40,600
+الحلقة هو دور بالفعل، يعني طيب بيقول meningitis أو
+
+80
+00:05:40,600 --> 00:05:44,680
+suspected meningitis، أنتم تتعرفوا على المسبب لـ
+
+81
+00:05:44,680 --> 00:05:48,840
+meningitis، وقد تستطيعوا أنكم تعطوا الطبيب معلومات 
+
+82
+00:05:48,840 --> 00:05:56,620
+أخرى مثل مدى حساسيتها للمضاد الحيوي أو مقاومته، إذن
+
+83
+00:05:56,620 --> 00:06:01,480
+في عندي أنا قائمة طويلة على اسم الإنسان من الأمراض
+
+84
+00:06:01,480 --> 00:06:04,140
+بتسببها الـ streptococcus، المرة الماضية أنا
+
+85
+00:06:04,140 --> 00:06:06,380
+قلت لكم معلومة، أنا قلت لكم الـ staphylococcus
+
+86
+00:06:06,380 --> 00:06:10,420
+aureus هي أكثر مايكروب هتعزله في الـ clinical
+
+87
+00:06:10,420 --> 00:06:14,120
+microbiology من الـ gram-positive، مش من كل البكتيريا، من
+
+88
+00:06:14,120 --> 00:06:17,830
+الـ gram-positive، لكن اليوم أنا بقول لكم إن الـ 
+
+89
+00:06:17,830 --> 00:06:20,350
+streptococcus، وهذا الكلام برضه حكيته المرة
+
+90
+00:06:20,350 --> 00:06:24,890
+الماضية بأكد عليه، إنه بتسبب أكثر أنواع من العدوى
+
+91
+00:06:24,890 --> 00:06:31,190
+أكثر أنواع، وليس more frequent، بيختلف المصطلحين هذه
+
+92
+00:06:31,190 --> 00:06:34,790
+أكثر حدوثًا من العدوى، هذه أكثر حدوثًا لأنواع
+
+93
+00:06:34,790 --> 00:06:39,250
+مختلفة من العدوى، كما نشاهد على اللوحة اللي أمامنا
+
+94
+00:06:39,250 --> 00:06:43,550
+group A streptococcal infection
+
+95
+00:06:46,300 --> 00:06:49,220
+Group A، وبعدين أنا أتكلم مع مين أنا؟ أتكلم على
+
+96
+00:06:49,220 --> 00:06:54,260
+نوع محدد من الـ streptococci، وقبل ما أبدأ فيه
+
+97
+00:06:54,260 --> 00:06:59,610
+الصحيح، لازم أنا أحاول أربط بين معلومتين، المعلومة
+
+98
+00:06:59,610 --> 00:07:02,810
+الأولى في التصنيف، قلنا Alpha, Beta, Gamma صح؟
+
+99
+00:07:02,810 --> 00:07:05,890
+المعلومة الثانية Group A, Group B, Group C لها
+
+100
+00:07:05,890 --> 00:07:08,870
+Serological Classification أو Lansfield
+
+101
+00:07:08,870 --> 00:07:11,650
+Classification، كلمة Serology يعني Antibody
+
+102
+00:07:11,650 --> 00:07:15,770
+وAntigen، واحنا قلنا أنه Lansfield صنفت الميكروبات
+
+103
+00:07:15,770 --> 00:07:20,050
+هذه Based on Surface Antigen، يبقى هو Serology أو
+
+104
+00:07:20,050 --> 00:07:23,570
+Serological Classification، الآن Group A فيها
+
+105
+00:07:23,570 --> 00:07:27,980
+مجموعة من الميكروبات، من الأسماء الوحيد اللي فيهم
+
+106
+00:07:27,980 --> 00:07:31,040
+الـ Beta-Hemolytic هي الـ Streptococcus pyogenes،
+
+107
+00:07:31,040 --> 00:07:34,360
+فماشي، جاروا الأسماء والأطباء، جاروا احنا، طيب لو
+
+108
+00:07:34,360 --> 00:07:37,840
+نَمَزْنَا two systems مع بعض، وجرّنا Group A Beta
+
+109
+00:07:37,840 --> 00:07:41,700
+-Hemolytic، احنا واقع الأمر حددنا هوية الكائن اللي 
+
+110
+00:07:41,700 --> 00:07:45,840
+بدناه، يعني اللي هو مين؟ streptococcus pyogenes، إذا
+
+111
+00:07:45,840 --> 00:07:50,160
+للأقل لو سمعتوا مصطلح بيقول Group A beta hemolytic
+
+112
+00:07:50,160 --> 00:07:53,980
+streptococci، احنا بنقصد مين؟ لـ streptococcus
+
+113
+00:07:53,980 --> 00:07:58,560
+pyogenes، ولحد الآن كثير كتب وكثير مراجعة will
+
+114
+00:07:58,560 --> 00:08:01,840
+refer to this organism as group A streptococci، حتى
+
+115
+00:08:01,840 --> 00:08:04,700
+بدون ما يقولوا beta hemolytic، لأنها اشتهرت بهذا
+
+116
+00:08:04,700 --> 00:08:12,900
+الاسم، بتصيب البشر في كل الأعمار، لكن أكثر فئة عمرية
+
+117
+00:08:12,900 --> 00:08:16,520
+مستهدفة من هذه البكتيريا هي الفئة العمرية من خمسة 
+
+118
+00:08:16,520 --> 00:08:21,820
+لخمس عشر سنة، أتكلمنا عن Group A streptococci و
+
+119
+00:08:21,820 --> 00:08:25,100
+اللي هي beta hemolytic، واتكلمنا عن streptococcus
+
+120
+00:08:25,100 --> 00:08:28,120
+pyogenes، تذكروا دائمًا أن الموضوع اللي بتتكلم فيه
+
+121
+00:08:28,120 --> 00:08:31,540
+هو البكتيريا المقيحة الصديدية اللي بتنتج
+
+122
+00:08:31,540 --> 00:08:36,800
+infection with pus، ممكن تعمل non-invasive diseases
+
+123
+00:08:36,800 --> 00:08:40,780
+زي الـ pharyngitis، وكلمة pharyngitis التهاب الحلق، والتهاب
+
+124
+00:08:40,780 --> 00:08:46,220
+الحلق بيشمله اللي هو الـ tonsillitis، skin infections
+
+125
+00:08:46,220 --> 00:08:49,100
+لما بقول skin infections بيكونوا يتذكروا أن ال- 
+
+126
+00:08:49,100 --> 00:08:52,360
+skin هو أكبر organ في جسم الإنسان وبالتالي احتمال
+
+127
+00:08:52,360 --> 00:08:55,880
+الإصابة من الرأس إلى أخمص القدم موجودة. الـ impetigo 
+
+128
+00:08:55,880 --> 00:08:57,920
+حكينا عنها اللي هي الحصف اللي يقول إنها مشترك مع
+
+129
+00:08:57,920 --> 00:09:01,920
+الـ staphylococcus aureus، فينا invasive bacterium 
+
+130
+00:09:01,920 --> 00:09:04,720
+toxic shock like syndrome. أعتقد أن احنا فهمنا الـ
+
+131
+00:09:04,720 --> 00:09:10,120
+toxic shock syndrome وامبارح أنا نزلتلكوا على الـ
+
+132
+00:09:10,120 --> 00:09:12,240
+... الـ ... الـ essential medical microbiology group
+
+133
+00:09:12,240 --> 00:09:15,540
+flash على الـ super antigen and how .. how they work
+
+134
+00:09:15,540 --> 00:09:20,000
+أرجو من حضراتكم أن تقضوا 3 دقائق فقط لن تنسوا الـ
+
+135
+00:09:20,000 --> 00:09:22,620
+mechanism of action أبدًا إذا رأيتم الـ flash
+
+136
+00:09:22,620 --> 00:09:26,130
+animation وكان فيه flash animation آخر آخر عن الـ
+
+137
+00:09:26,130 --> 00:09:29,410
+phagocytosis برضه كيف الـ macrophage يبتعمل 
+
+138
+00:09:29,410 --> 00:09:32,650
+phagocytosis وكيف يبتعمل processing للـ antigen
+
+139
+00:09:32,650 --> 00:09:35,570
+وكيف يبتعمل الـ antigen presentation فيها. تفرجوا 
+
+140
+00:09:35,570 --> 00:09:39,410
+عليها. flash eating bacteria الصورة هذه اللي
+
+141
+00:09:39,410 --> 00:09:42,790
+قدامكوا هي الألطف على الإطلاق. هي الألطف على
+
+142
+00:09:42,790 --> 00:09:46,050
+الإطلاق من الصور الموجودة على الجوجل. يعني لو
+
+143
+00:09:46,050 --> 00:09:48,070
+عملتوا google search على الـ flash eating bacteria
+
+144
+00:09:48,070 --> 00:09:53,920
+هتشوفوا أشكال مخيفة كثيرة جدًا. نفس البكتيريا اللي
+
+145
+00:09:53,920 --> 00:09:56,380
+تمالك تونسولايتل تمالك التهيبط اللواز هي نفس
+
+146
+00:09:56,380 --> 00:09:59,820
+البكتيريا اللي تمال فلش eating بكتيريا. الفرق بينهم
+
+147
+00:09:59,820 --> 00:10:05,600
+أن هذه أصيبت ببكتيريوفاج. فاكرين لبروفاج اللي بيعمل
+
+148
+00:10:05,600 --> 00:10:09,820
+integration لنفسه داخل البكتيريا. أعطى البكتيريا
+
+149
+00:10:09,820 --> 00:10:15,060
+المقدرة على إنتاج البروتياز بكميات كبيرة. بروتيايز
+
+150
+00:10:15,060 --> 00:10:19,520
+يعني أو بروتيايز يعني إنزيمات هاضمة للبروتين
+
+151
+00:10:19,520 --> 00:10:24,570
+واللحم عبارة عن البروتين. وبالتالي بنلاقي إنه الـ
+
+152
+00:10:24,570 --> 00:10:28,270
+infection is very severe والـ consequences تبعتها
+
+153
+00:10:28,270 --> 00:10:32,910
+برضه بتكون damaging. يعني أحد الأشخاص المصابين في
+
+154
+00:10:32,910 --> 00:10:37,590
+الوجه بنشوف الأسنان من جوا. بصير destruction
+
+155
+00:10:37,590 --> 00:10:42,030
+ لكميات كبيرة من الـ muscles ومن الـ tissues و
+
+156
+00:10:42,030 --> 00:10:44,610
+بالتالي هو يعتبر من الأمراض الخطيرة والـ deadly
+
+157
+00:10:44,610 --> 00:10:49,130
+disease. كلمة pyrogenic toxin أظن بيوجينيك غير عن
+
+158
+00:10:49,130 --> 00:10:53,250
+بيروجين. بيروجين اللي هي fever producing والـ bio
+
+159
+00:10:53,250 --> 00:10:58,110
+gene هي الـ pass producing. الـ pyrogenic toxin هو
+
+160
+00:10:58,110 --> 00:11:02,730
+يفرز عن super antigen وكلمة super antigen يعني من 
+
+161
+00:11:02,730 --> 00:11:06,490
+ميزاته أنه بيحفز الخلايا على الانقسام فبسموه
+
+162
+00:11:06,490 --> 00:11:10,210
+mitogen وهذا بيعمل activation للـ immune system
+
+163
+00:11:10,210 --> 00:11:13,590
+أعتقد كلكوا شوفته أنه بيعمل non specific
+
+164
+00:11:13,590 --> 00:11:18,780
+activation للـ immune system. في مرض اسمه الحمى
+
+165
+00:11:18,780 --> 00:11:22,980
+القرمزيّة أو scarlet fever مشهور برضه متسببه لـ
+
+166
+00:11:22,980 --> 00:11:27,420
+streptococcus pyogenes ونتج عن مقدرة البكتيريا أو
+
+167
+00:11:27,420 --> 00:11:30,580
+عن استرالة هالية على إنتاج توكسين اسمه eretrogenic
+
+168
+00:11:30,580 --> 00:11:36,760
+toxin. الاسم تبع المرض أخذ من لون المريض، القرمزي،
+
+169
+00:11:36,760 --> 00:11:39,600
+لون المريض. وفيه طبعًا فيه fever وفيه بالإضافة اللي
+
+170
+00:11:39,600 --> 00:11:42,820
+هو الـ rashes اللي أنتو شايفينها اللي قد تظهر على
+
+171
+00:11:42,820 --> 00:11:46,080
+اللسان. مش قد تظهر. مميزة من ميزته أن تكون على لسان
+
+172
+00:11:46,080 --> 00:11:51,260
+المريض أيضًا فيه rashes غير
+
+173
+00:11:51,260 --> 00:11:56,340
+infections غير قيحية. هذه بدنا نفهمها كويس في سياق
+
+174
+00:11:56,340 --> 00:12:02,260
+البكتيريا موجودة ممكن تعمل حاجة من اثنين. تعمل 
+
+175
+00:12:02,260 --> 00:12:03,380
+local infection
+
+176
+00:12:06,330 --> 00:12:10,610
+وممكن تعمل الـ focal suppurative. suppurative يعني برضه
+
+177
+00:12:10,610 --> 00:12:13,810
+pus producing. نفس المعنى تبع بيوجينيك تمام؟ أو
+
+178
+00:12:13,810 --> 00:12:20,070
+focal suppurative لكن هذه البكتيريا نفسها لكن قد ينتج
+
+179
+00:12:20,070 --> 00:12:25,250
+أمراض عن موجود بقايا البكتيريا أو عن آثار
+
+180
+00:12:25,250 --> 00:12:29,510
+البكتيريا. آثار المعركة اللي موجودة، حين تجانبها
+
+181
+00:12:29,510 --> 00:12:31,270
+رجزوا مياه وتقولوا الله وحلوة
+
+182
+00:12:34,040 --> 00:12:36,300
+الآن الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ
+
+183
+00:12:36,300 --> 00:12:36,620
+الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ
+
+184
+00:12:36,620 --> 00:12:37,960
+الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ
+
+185
+00:12:37,960 --> 00:12:40,520
+الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ
+
+186
+00:12:40,520 --> 00:12:44,060
+الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ
+
+187
+00:12:44,060 --> 00:12:47,740
+الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ
+
+188
+00:12:47,740 --> 00:12:50,920
+الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ
+
+189
+00:12:50,920 --> 00:12:51,700
+الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ الـ
+
+190
+00:12:51,700 --> 00:12:51,820
+الـ الـ
+
+191
+00:13:01,360 --> 00:13:06,180
+خلينا من أول نقطة. عرفنا إنه autoimmune disease
+
+192
+00:13:06,180 --> 00:13:09,960
+ناتج مش عن البكتيريا مباشرة وإنما عن reaction تبع
+
+193
+00:13:09,960 --> 00:13:14,300
+جسمنا ضد البكتيريا. وبيطلقوا عليه autoimmune
+
+194
+00:13:14,300 --> 00:13:18,040
+disease لأن our own antibody attacks our own
+
+195
+00:13:18,040 --> 00:13:24,120
+antigen وبتسموه autoimmune disease. بعنوان rheumatic
+
+196
+00:13:24,120 --> 00:13:27,540
+fever. أخذوا بالكم rheumatic fever مش rheumatoid
+
+197
+00:13:27,540 --> 00:13:30,960
+arthritis. الـ rheumatoid arthritis مرض
+
+198
+00:13:30,960 --> 00:13:34,740
+بيختلف عن الـ rheumatic fever. فالـ rheumatic fever
+
+199
+00:13:34,740 --> 00:13:39,920
+بنلاقي طفل عمره خمسة أعوام أو عشرة أعوام من
+
+200
+00:13:39,920 --> 00:13:43,380
+repeated infection with streptococcus pyogenes
+
+201
+00:13:44,000 --> 00:13:49,700
+المفاصل تبعت عمرها تسعين سنة. القلب تبعت عمره 
+
+202
+00:13:49,700 --> 00:13:52,700
+تسعين سنة لأن الـ antibodies بتهاجم أدلة القلب و
+
+203
+00:13:52,700 --> 00:13:56,120
+بتهاجم اللي هو الـ myosin بالذات اللي في ورقة أدلة 
+
+204
+00:13:56,120 --> 00:14:00,660
+القلب وبتهاجم المفاصل تبعت هذا المريض. إذا هي
+
+205
+00:14:00,660 --> 00:14:05,440
+عبارة عن antibody attacking our own tissues. وصل
+
+206
+00:14:05,440 --> 00:14:09,700
+الصورة. قد ينتج عنها fever و heart disease و joint
+
+207
+00:14:09,700 --> 00:14:14,270
+inflammation. وآخر جملة على اللوحة بتقول: rheumatic
+
+208
+00:14:14,270 --> 00:14:18,010
+fever not rheumatoid arthritis عشان نأكد أن دول
+
+209
+00:14:18,010 --> 00:14:21,570
+المرضين مختلفين. تبعنا طب أساس هو ذات نفسه المرض
+
+210
+00:14:21,570 --> 00:14:27,650
+هذه الـ fever مزمن ولا إنه rheumatoid مزمن؟ هو ...
+
+211
+00:14:27,650 --> 00:14:32,890
+هو بيعمل الـ damage مزمن. الـ damage تبعه مزمن بمعنى
+
+212
+00:14:32,890 --> 00:14:37,000
+آخر damage يعني irreversible. يعني لما بيحضر من دم
+
+213
+00:14:37,000 --> 00:14:41,200
+العضلات أو من دم الانسجة في أدات القلب، قد يختفي
+
+214
+00:14:41,200 --> 00:14:44,020
+الـ antibody بعد فترة. بيصير له نوع من الـ waning
+
+215
+00:14:44,020 --> 00:14:49,340
+يعني بيقل تركيزه، لكن الظرر تبعه بيضل موجود لدرجة
+
+216
+00:14:49,340 --> 00:14:52,740
+أن الـ rheumatic fever عن الـ M-proteins كيف بأنه
+
+217
+00:14:52,740 --> 00:14:56,000
+بيعمل cross-reaction مع الـ heart ميوسين وسمينا
+
+218
+00:14:56,000 --> 00:15:00,180
+وقلنا المصطلح الـ disease autoimmunity. في مشكلة
+
+219
+00:15:00,180 --> 00:15:03,200
+ثانية يا بنات. عندنا مشكلة ثانية في الـ ... الـ ... الـ
+
+220
+00:15:03,200 --> 00:15:06,200
+cell wall antigens، مش في الخلية نفسها وإنما في
+
+221
+00:15:06,200 --> 00:15:11,340
+بقاياها. poorly digested in vivo و persist
+
+222
+00:15:11,340 --> 00:15:15,510
+indefinitely. مية عن مية بتتكلم عن الـ cell wall
+
+223
+00:15:15,510 --> 00:15:20,050
+antigens. أن الـ digestion تبعها مش complete مش
+
+224
+00:15:20,050 --> 00:15:23,710
+complete يعني لا تتحلل إلى العناصر المكونة لها
+
+225
+00:15:23,710 --> 00:15:30,810
+رقم اثنين it can persist تبقى indefinitely. إيش
+
+226
+00:15:30,810 --> 00:15:34,090
+يعني indefinitely زي ما بنقول إحنا لأخر يعني ما لهاش
+
+227
+00:15:34,090 --> 00:15:38,210
+إلى ما لها نهاية. إلى ما لها نهاية. طب هذا إيش بيقدي
+
+228
+00:15:38,210 --> 00:15:44,190
+بيقدي إلى مشكلة أخرى. تعالوا نطلع على المشكلة
+
+229
+00:15:44,190 --> 00:15:49,490
+الأخرى. الـ antigen لما بيرتبط بالـ antibody فاكرين
+
+230
+00:15:49,490 --> 00:15:54,930
+بقولنا أن الـ binding قد يسبب ما يسمى complement
+
+231
+00:15:54,930 --> 00:16:00,570
+activation. complement activation. أذكركم الـ
+
+232
+00:16:00,570 --> 00:16:01,050
+complement
+
+233
+00:16:21,170 --> 00:16:25,330
+بقايا الانتيجنات مرتبطة بالـ antibodies وبتلف في
+
+234
+00:16:25,330 --> 00:16:29,800
+الدم. لما تلف في الدم مش الدم كله بيروح للكلية. لما
+
+235
+00:16:29,800 --> 00:16:32,820
+هذا الـ antigen-antibody complex كمان مرة نسميه
+
+236
+00:16:32,820 --> 00:16:36,920
+antigen-antibody complex وبنسميه هنا زي ما
+
+237
+00:16:36,920 --> 00:16:41,040
+شايفينها اللوحة immune complex. immune complex. الـ
+
+238
+00:16:41,040 --> 00:16:43,980
+immune complexes كمان مرة هي الـ antigen-antibody
+
+239
+00:16:43,980 --> 00:16:49,940
+binding. بتترستر في الكلمة. طيب هو عبارة عن antigen
+
+240
+00:16:49,940 --> 00:16:53,800
+مرتبط بـ antibody هي سبب activation لمين؟ للـ
+
+241
+00:16:53,800 --> 00:16:57,480
+complement. فالـ complement مفيش bacteria هتدمر مين؟
+
+242
+00:16:57,760 --> 00:17:00,900
+هتدمر خلايا الكلية عشان هي اسم المرض acute
+
+243
+00:17:00,900 --> 00:17:06,100
+glomerulonephritis. كمان مرة فيه بكتيريا هناك. طيب
+
+244
+00:17:06,100 --> 00:17:10,340
+الـ acute glomerulonephritis هيشهر المريض بأعراض الـ
+
+245
+00:17:10,340 --> 00:17:13,680
+kidney infection لكن مفيش infection عملته مزرعة
+
+246
+00:17:13,680 --> 00:17:18,140
+negative. ما عنده أي حاجة. السبب هو مين؟ هو الـ immune
+
+247
+00:17:18,140 --> 00:17:22,280
+complexes ومهاجمة الـ complement كمان مرة لخلايا الـ
+
+248
+00:17:22,280 --> 00:17:27,090
+kidney. وزي الصورة إذا شفنا مرضين acute glomerul
+
+249
+00:17:27,090 --> 00:17:30,650
+nephritis وشوفنا rheumatic fever. هدول الاثنين أو
+
+250
+00:17:30,650 --> 00:17:35,190
+الاثنين ليس لهم علاقة مباشرة بالبكتيريا وإنما بال 
+
+251
+00:17:35,190 --> 00:17:40,350
+immunity تبعتنا ضد اللي هو البكتيريا و إن cross
+
+252
+00:17:40,350 --> 00:17:44,450
+reaction يعني antibody بدل ما يتفاعل مع antigen
+
+253
+00:17:44,450 --> 00:17:50,320
+واحد يتفاعل مع two أو more antibodies فمصدرها cross
+
+254
+00:17:50,320 --> 00:17:53,740
+reaction ما يكون عارفينه أنه مؤشر على أن ال
+
+255
+00:17:53,740 --> 00:17:57,820
+antibody أو ال antigen بتفاعل مع أكثر من نظيرهم
+
+256
+00:17:57,820 --> 00:18:03,700
+نيجي للميكروب التاني خذوا بالكم من أهم الميكروبات
+
+257
+00:18:03,700 --> 00:18:06,360
+في ال streptococcus اللي ذكرناها في السابق لها
+
+258
+00:18:06,360 --> 00:18:07,840
+streptococcus pyogenes
+
+259
+00:18:10,280 --> 00:18:13,620
+نقول إن ال group ممكن يكون فيها واحد أو أكثر، هذه
+
+260
+00:18:13,620 --> 00:18:19,080
+ال group دي لا تمتلك إلا عنصر واحد، اسمها
+
+261
+00:18:19,080 --> 00:18:20,860
+streptococcus agalactiae
+
+262
+00:18:23,710 --> 00:18:27,110
+عشان أكتب لكم يعني الأمراض اللي بتسببها مكتوبة
+
+263
+00:18:27,110 --> 00:18:30,630
+neonatal meningitis و septicemia و pneumonia أول مرة
+
+264
+00:18:30,630 --> 00:18:34,810
+نشوف الترتيب بعكس 3 2 1 يعني المفروض أنه
+
+265
+00:18:34,810 --> 00:18:41,650
+واحد اثنين ثلاثة لكن هي الصحيح مكتوبة ب .. بقصد أن
+
+266
+00:18:41,650 --> 00:18:45,470
+نقول لكم pneumonia هي البدء أو نقطة الانطلاق لل
+
+267
+00:18:45,470 --> 00:18:49,490
+septicemia و ال septicemia هي نقطة الانطلاق لمن؟ ل
+
+268
+00:18:49,490 --> 00:18:53,490
+neonatal meningitis لكن حاطينهم بالترتيب اللي ..
+
+269
+00:18:53,490 --> 00:18:57,230
+اللي فوق لأنه .. الأخطر هو من؟ ال .. meningitis
+
+270
+00:18:57,230 --> 00:18:59,830
+أخطر من ال septicemia أخطر طبعًا من اللي هو ال pneumonia
+
+271
+00:18:59,830 --> 00:19:04,070
+لكن واقع الأمر هي pneumonia بينشأ عنها septicemia
+
+272
+00:19:04,070 --> 00:19:09,030
+بينشأ عنها قلنا meningitis في كمان مرض بيصيب الأم
+
+273
+00:19:09,030 --> 00:19:13,410
+منها peripheral sepsis و أول مرة ممكن نقولها لأ
+
+274
+00:19:13,410 --> 00:19:18,140
+قلنا قبل هيك ال puerperal sepsis هو حمى النفاس، حمى 
+
+275
+00:19:18,140 --> 00:19:22,740
+النفاس، كنتوا تسمعوا .. مش تسمعوا، ممكن في زمن كنا
+
+276
+00:19:22,740 --> 00:19:26,260
+لأ مش موجود، بس كثير من النساء كانت تموت بعد
+
+277
+00:19:26,260 --> 00:19:31,020
+الولادة بحمى النفاس، و بقى جميعهم حمى ثلاث أيام،
+
+278
+00:19:31,020 --> 00:19:33,540
+إنت بقى كانت تيجي بعد ثلاث أيام من الولادة، بعد ثلاث
+
+279
+00:19:33,540 --> 00:19:38,260
+أيام من الولادة نسبة كبيرة جدًا من الأمهات، مش أنا
+
+280
+00:19:38,260 --> 00:19:41,420
+بس في فلسطين، في العالم كله، لكن بعد ما صارت ال
+
+281
+00:19:41,420 --> 00:19:44,560
+infection control measures و ال antibiotics، هذه
+
+282
+00:19:44,560 --> 00:19:49,860
+تقريبًا وصلت أنا إلى zero وفيات الأمهات خلال
+
+283
+00:19:49,860 --> 00:19:55,920
+الولادة، الناجمة عن ال peripheral sepsis طيب هذا من
+
+284
+00:19:55,920 --> 00:19:59,560
+الميكروبات المهمة في فلسطين و أنا كمان مرة بقول
+
+285
+00:19:59,560 --> 00:20:05,680
+أنه بنحاول قدر الإمكان أنه ننبه الأطباء خاصة أطباء
+
+286
+00:20:05,680 --> 00:20:09,140
+النساء والولادة إيه أهمية هذا الميكروب في الاهتمام
+
+287
+00:20:09,140 --> 00:20:14,300
+الأمومة و الطفولة بنقول لهم التالي، بنقول لهم الست
+
+288
+00:20:14,300 --> 00:20:17,840
+الحامل في الشهر السادس و ثم فوق إيه اللي بقى أن
+
+289
+00:20:17,840 --> 00:20:23,490
+تخضع لفحص؟ هذا الفحص لموجود أو عدم موجود ل group B
+
+290
+00:20:23,490 --> 00:20:26,450
+streptococci اسمها streptococcus agalactiae من
+
+291
+00:20:26,450 --> 00:20:33,830
+vaginal swab مزرعة و بنتعرف على البكتيريا موجودة
+
+292
+00:20:33,830 --> 00:20:38,170
+بنحط بس تشك إنها positive مش موجودة بنحط تشك إنها
+
+293
+00:20:38,170 --> 00:20:41,910
+negative ما بنعملش ولا أي إجراء و بننتظر ليوم
+
+294
+00:20:41,910 --> 00:20:46,070
+الولادة يوم الولادة بيعطي ما يسمى بالكلمة خلال
+
+295
+00:20:46,070 --> 00:20:52,050
+الولادة معناها intrapartum هذا مصطلح بيطلق على خلال
+
+296
+00:20:52,050 --> 00:20:55,550
+الولادة فلو أعطينا Antibiotic زي البنسلين خلال
+
+297
+00:20:55,550 --> 00:21:00,260
+الولادة إحنا نضرب على الصفر إن شاء الله نحمي الأم من
+
+298
+00:21:00,260 --> 00:21:03,100
+الـ Periperal Sepsis لأن إنت عارفين عمليات الولادة
+
+299
+00:21:03,100 --> 00:21:09,020
+عملية عنيفة دم و نزف و جروح ال cervix من بلاي تتهتك
+
+300
+00:21:09,020 --> 00:21:13,500
+كثير منها يعني عملية عنيفة بالفعل و الدم اللي بينزل
+
+301
+00:21:13,500 --> 00:21:17,060
+هذا بينزل من الكبلارز و من .. من .. مظبوط و بالتالي في
+
+302
+00:21:17,060 --> 00:21:21,180
+إمكانية أنه يصير تلوث و أن البكتيريا تدخل دم عند
+
+303
+00:21:21,180 --> 00:21:27,360
+الأم اللي هي في الولادة فوجود ال antibiotic بيحمي الأم
+
+304
+00:21:27,360 --> 00:21:33,300
+من ال puerperal sepsis و بيحمي الطفل المولود من أنه يعمل
+
+305
+00:21:33,300 --> 00:21:37,100
+.. يصير له لو عنده colonization استعمار من قبل ال
+
+306
+00:21:37,100 --> 00:21:41,000
+group B streptococci للطفل و يبتدي لأن يحدث pneumonia
+
+307
+00:21:41,000 --> 00:21:45,040
+ولا يحدث septicemia ولا يحدث إيه .. من .. للأسف ال
+
+308
+00:21:45,040 --> 00:21:47,740
+practice هذا غير موجود بتة
+
+309
+00:21:52,160 --> 00:21:55,780
+في قطاع غزة مش موجود بتة و بالتالي نأمل من الله
+
+310
+00:21:55,780 --> 00:22:00,320
+أنه ينتبه لهذا الموضوع ليش إحنا ركزنا على ست
+
+311
+00:22:00,320 --> 00:22:04,820
+الحامل؟ لأنه 20 ل 30% من الستات بيحتووا على هذا
+
+312
+00:22:04,820 --> 00:22:09,500
+الميكروب ك normal microbiota normal microbiota
+
+313
+00:22:09,500 --> 00:22:13,360
+يعني هو غير مضر اللي هم خلال قبل الولادة لكن يوم
+
+314
+00:22:13,360 --> 00:22:18,120
+الولادة بيصير مضر لا للست ولا اللي هو المولود اللي
+
+315
+00:22:18,120 --> 00:22:22,050
+طالب إذا ال transmission هو عبر ال vagina الفلورة
+
+316
+00:22:22,050 --> 00:22:25,450
+during birth during birth إنت بتعرف اليوم صار في
+
+317
+00:22:25,450 --> 00:22:27,790
+حين اسمها caesarean section اللي هو الولادة
+
+318
+00:22:27,790 --> 00:22:33,250
+القيصرية و صارت شائعة بس أنبئكم بنقطة البعض يظن
+
+319
+00:22:33,250 --> 00:22:39,410
+و كان يظن أن الولادة القيصرية بتحمي الطفل من ال
+
+320
+00:22:39,410 --> 00:22:43,610
+infection لأن ما بيمرّش عبر من؟ عبر ال vagina اللي هي
+
+321
+00:22:43,610 --> 00:22:47,140
+مليانة لهم الملك اللي هو بيوتهم صح؟ لكن ورد أنه
+
+322
+00:22:47,140 --> 00:22:52,560
+.. ورد أنه نسبة الإصابة للأطفال اللي بيولدوا من
+
+323
+00:22:52,560 --> 00:22:56,100
+ال C-section اللي هو cesarean section هذا إما
+
+324
+00:22:56,100 --> 00:23:01,100
+مساوية للأطفال اللي بيولدوا ولادة طبيعية أو حتى
+
+325
+00:23:01,100 --> 00:23:04,440
+أعلى منها في دراسة إحنا عملناها مع طالب بالستر و
+
+326
+00:23:04,440 --> 00:23:08,460
+أظهر أنه الناس اللي بيولدوا من ال C-section نسبة
+
+327
+00:23:08,460 --> 00:23:12,960
+ال neonatal septicemia عندهم أعلى من الأطفال اللي
+
+328
+00:23:12,960 --> 00:23:15,640
+بيولدوا ولادة طبيعية normal delivery
+
+329
+00:23:18,270 --> 00:23:21,170
+سألت سؤال إحنا ما قدرناش نجاوب عليه في ال research
+
+330
+00:23:21,170 --> 00:23:24,950
+لأنه هو سؤال محير المنطق أن يكون العكس .. المنطق أن
+
+331
+00:23:24,950 --> 00:23:29,530
+يكون العكس لكن يبدو أن ال procedures .. ال .. ال
+
+332
+00:23:29,530 --> 00:23:32,510
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+333
+00:23:32,510 --> 00:23:32,770
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+334
+00:23:32,770 --> 00:23:33,090
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+335
+00:23:33,090 --> 00:23:37,330
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+336
+00:23:37,330 --> 00:23:38,030
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+337
+00:23:38,030 --> 00:23:39,030
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+338
+00:23:39,030 --> 00:23:41,470
+ال .. ال .. ال ..
+
+339
+00:23:44,100 --> 00:23:48,640
+من الأول اللي بيطلع .. أول نفس بياخذه .. أول ..
+
+340
+00:23:48,640 --> 00:23:52,200
+أول يد تمسكه بتعطيه micro organism .. تمام؟ من
+
+341
+00:23:52,200 --> 00:23:56,160
+أول ملامسة الجلد .. جلد الأم عند الرضاعة أو ال
+
+342
+00:23:56,160 --> 00:24:00,260
+bottle تبع الحليب بياخد micro organism طبعًا مطلوب
+
+343
+00:24:00,260 --> 00:24:03,020
+أنه ياخد micro organism هو بالعكس أنا أقول لكم أن
+
+344
+00:24:03,020 --> 00:24:06,780
+ال micro bad في هذه الحالة إذا كانت في environment
+
+345
+00:24:06,780 --> 00:24:11,910
+healthy بتكون مفيدة للأطفال اللي أنا بدأت أقوله أن
+
+346
+00:24:11,910 --> 00:24:16,370
+إحنا البعض مننا بيسوق لمن؟ ل caesarean section لأ
+
+347
+00:24:16,370 --> 00:24:19,630
+بيقول لكم caesarean section فقط المفروض فقط في
+
+348
+00:24:19,630 --> 00:24:24,970
+حالات الولادة المتعثرة مش يعني .. مش أفضل من ال
+
+349
+00:24:24,970 --> 00:24:31,350
+normal delivery طيب streptococcus agalactiae
+
+350
+00:24:31,350 --> 00:24:35,770
+هويتها إيطالية برضه streptococcus agalactiae طبعًا
+
+351
+00:24:35,770 --> 00:24:39,870
+فيه اختصار اللي هي ال S.
+
+352
+00:24:48,120 --> 00:24:50,640
+عشان هيك كنت أتعرف أن الاقتصاد بتتشابه هي
+
+353
+00:24:50,640 --> 00:24:53,680
+ال staphylococcus مع ال staphylococcus التاني أجي و
+
+354
+00:24:53,680 --> 00:24:57,380
+حرف اللي هو ال S طيب الصورة اللي قدامنا هي صورة
+
+355
+00:24:57,380 --> 00:25:01,840
+رائعة جدًا و هتضطر تخلينا نتكلم موضوع خارج نطاق
+
+356
+00:25:01,840 --> 00:25:09,360
+المحاضرة لكن برضه في ال micro biology لما
+
+357
+00:25:09,360 --> 00:25:13,060
+بنقول واحد زائد واحد إحنا غالبًا الجواب اللي بيتبادل
+
+358
+00:25:13,060 --> 00:25:17,720
+للدهن عنّا على طول اثنين مش غالبًا دائمًا اثنين هذا دي
+
+359
+00:25:17,720 --> 00:25:22,460
+المعرفة أن واحد زائد واحد يساوي اثنين طيب انجليها
+
+360
+00:25:22,460 --> 00:25:26,160
+في صيغة ثانية أنت بتكتب 100 كلمة في الدقيقة و يا
+
+361
+00:25:26,160 --> 00:25:30,580
+بتكتب 100 كلمة في الدقيقة لو قلنا لكم اكتبوا كلمات
+
+362
+00:25:30,580 --> 00:25:35,480
+مجموع كلماتكم هيطلع كده 200 في الدقيقة يبقى واحد زائد
+
+363
+00:25:35,480 --> 00:25:39,540
+واحد يساوي اثنين صح ولا لأ؟ طيب لو قلنا لهم اكتبوا
+
+364
+00:25:39,540 --> 00:25:45,420
+مرة ثانية مثال ثاني اثنين غير 100 و 100 فواحدة منهم
+
+365
+00:25:45,420 --> 00:25:51,260
+اشتغلت على الثانية يعني عاقة عملها بدل ما الاثنين
+
+366
+00:25:51,260 --> 00:25:57,420
+يكتبوا 200 يمكن يكتبوا عاقة على عملها ممكن يكتبوا
+
+367
+00:25:57,420 --> 00:26:02,340
+أقل من 100 أو أقل من 200 لكن في الحالة واحد زائد
+
+368
+00:26:02,340 --> 00:26:06,100
+واحد ده يساوي اثنين لأن في تباين في ال action نجي
+
+369
+00:26:06,100 --> 00:26:11,910
+لأ مثال ثالث نفسي لو شخصين كنت لهم اكتبوا 100 100
+
+370
+00:26:11,910 --> 00:26:16,990
+تعاونوا مع بعض جالهم .. حكى أنه الكلمات هذه
+
+371
+00:26:16,990 --> 00:26:20,490
+المقررة اللي أنا بعملها أو أنا عندي طريقة أسرع على
+
+372
+00:26:20,490 --> 00:26:24,810
+الكتابة خذ تعلم هذه التقنية، المهم في الآخر لما
+
+373
+00:26:24,810 --> 00:26:28,130
+جينا جمعنا الكلمات لجينا لهم 300، واحد زائد
+
+374
+00:26:28,130 --> 00:26:30,410
+واحد بطلوا .. بطلوا اثنين، بطلوا اثنين، صاروا
+
+375
+00:26:30,410 --> 00:26:35,150
+ثلاثة ففي عندنا في العلاقات في المركبات لما 
+
+376
+00:26:35,150 --> 00:26:38,050
+بيقوموا بـ action واحد يعني مُركّبين هم جايبين 
+
+377
+00:26:38,050 --> 00:26:42,330
+بيطبعوا كلمات مُركّبين بيعملوا homolysis واحد
+
+378
+00:26:42,330 --> 00:26:45,750
+بيعمل homolysis وضفناله كمان واحد بيعمل homolysis
+
+379
+00:26:45,750 --> 00:26:50,130
+المنطق أنه واحد زاد واحد ساوي اثنين واحد ده موجود
+
+380
+00:26:50,130 --> 00:26:52,890
+لكن هناك بعض الأحيان بنلجأ لحالة اسمها synergism
+
+381
+00:26:52,890 --> 00:26:59,530
+تآذر تآذر يعني تعاون وبيسميها synergism كمان
+
+382
+00:26:59,530 --> 00:27:07,340
+مرضياش؟ Synergism, Antagonism and Addition نعرفهم
+
+383
+00:27:07,340 --> 00:27:14,040
+ بالأرقام ونقول synergism واحد زائد
+
+384
+00:27:14,040 --> 00:27:20,840
+واحد يساوي هو المفروض أن نقول  الضبط أكثر من
+
+385
+00:27:20,840 --> 00:27:25,460
+اثنين لكن لنحفظهم ونسميهم ثلاثة Antagonism واحد
+
+386
+00:27:25,460 --> 00:27:30,520
+زائد واحد يساوي واحد الـ addition واحد 
+
+387
+00:27:34,240 --> 00:27:37,580
+هيك بيكون هنا أجددنا أن نعرف العلاقات بين
+
+388
+00:27:37,580 --> 00:27:41,200
+مُركّبين بيقوم نفس الوظيفة وين بهمنا الكلام هذا؟
+
+389
+00:27:41,200 --> 00:27:45,940
+بيهمنا كمان في الـ antibiotics في الـ antibiotics في
+
+390
+00:27:45,940 --> 00:27:50,940
+عندنا antibiotic قد يُعطى in combination مع آخر
+
+391
+00:27:50,940 --> 00:27:56,590
+antibiotic ممكن للأسف .. للأسف إذا ما كناش عارفين
+
+392
+00:27:56,590 --> 00:27:59,510
+العلاقات الثلاث هدول اللي حكينا عنهم نعطي اثنين
+
+393
+00:27:59,510 --> 00:28:03,810
+يعملوا تضاد بدل ما يساعدوا بعض ويخففوا من ال ..
+
+394
+00:28:03,810 --> 00:28:07,150
+من .. من وقت ال .. العلاج أو يقللوا وقت العلاج لأ
+
+395
+00:28:07,150 --> 00:28:09,870
+ممكن يعملوا تضاد ويزيدوا .. ويزيدوا من حتة دي
+
+396
+00:28:09,870 --> 00:28:14,310
+المشكلة وبالتالي نعرف المصطلحات الثلاث synergism
+
+397
+00:28:14,310 --> 00:28:17,970
+تآذر antagonism تضاد و addition اللي هو قلنا عنه
+
+398
+00:28:17,970 --> 00:28:20,850
+ال .. ال .. الإضافة نسميها إضافة
+
+399
+00:28:26,040 --> 00:28:29,680
+طيب، إيش اللي اخذناه يتكلم بهذا الكلام؟ اللي اخذناه
+
+400
+00:28:29,680 --> 00:28:35,220
+يتكلم بهذا الكلام هو وجود علاقة وطيدة ما بين الـ
+
+401
+00:28:35,220 --> 00:28:39,860
+Beta-Hemolysin الخاص بالـ Streptococcus Agalactica
+
+402
+00:28:39,860 --> 00:28:43,000
+والـ Beta-Hemolysin تبع الـ Streptococcus Aureus
+
+403
+00:28:43,830 --> 00:28:47,690
+قالوا لنا أن الـ Beta-Hemolysin بتاع الأجلاكتيا جامع
+
+404
+00:28:47,690 --> 00:28:54,050
+سينرجزم سينرجزم يعني تأثير مع الـ Staphylococcus
+
+405
+00:28:54,050 --> 00:28:57,550
+aureus ولحظوا الرسم اللي قدامكم هذه البكتيريا اللي
+
+406
+00:28:57,550 --> 00:29:01,010
+مزروعة هنا على الخط الطويل هذا هي Staphylococcus
+
+407
+00:29:01,010 --> 00:29:06,490
+aureus في بكتيريا أخرى زرعناها بشكل عمودي متعامد
+
+408
+00:29:06,490 --> 00:29:11,050
+عليها اللي هي Streptococcus Agalactica وواحدة كنا
+
+409
+00:29:11,050 --> 00:29:14,150
+شاكين أنها تكون Streptococcus Agalactica لحظوا
+
+410
+00:29:14,150 --> 00:29:19,430
+منطقة الالتقاء بين الاثنين إيش عملت؟ عملت ما يسمى
+
+411
+00:29:19,430 --> 00:29:22,870
+Arrowhead Formation عملت زي رأس السهم Arrowhead
+
+412
+00:29:22,870 --> 00:29:26,570
+Formation يعني الـ zone .. الـ zone تبعت الـ
+
+413
+00:29:26,570 --> 00:29:29,830
+hemolysis زادت ولا نقصت؟ زادت وبالتالي هذا
+
+414
+00:29:29,830 --> 00:29:33,590
+synergism ولا antagonism؟ synergism هذه الصفة
+
+415
+00:29:33,590 --> 00:29:38,270
+.. هذه الصفة تعتبر صفة مميزة للـ Streptococcus أجل
+
+416
+00:29:38,270 --> 00:29:43,420
+أجل .. إذن أحد أهم الصفات اللي بنعرف من خلالها الـ
+
+417
+00:29:43,420 --> 00:29:47,220
+agalactia هي الصفة دي إيش اسم الصفة دي؟ Camp test
+
+418
+00:29:47,220 --> 00:29:53,700
+Camp test أو Camp reaction طبعًا Camp هي عبارة عن
+
+419
+00:29:53,700 --> 00:29:58,860
+أربع أحرف لأربع علماء شاركوا فيها ده ده ده فالمسمى
+
+420
+00:29:58,860 --> 00:30:05,100
+هنا Camp reaction أو Camp test وانتهى الموضوع طيب
+
+421
+00:30:05,100 --> 00:30:10,560
+البكتيريا الثانية هذه اللي تحت عملت synergism؟
+
+422
+00:30:13,150 --> 00:30:17,570
+يبقى مستحيل تكون أجلكت طب في فحص ثاني اسمه
+
+423
+00:30:17,570 --> 00:30:21,330
+hypurate hydrolysis، hypurate hydrolysis، لحظة
+
+424
+00:30:21,330 --> 00:30:26,390
+الأنابيب اللي هنا، في عندي واحدة لونها تقريبًا
+
+425
+00:30:26,390 --> 00:30:33,270
+نفسي، واحدة colorless، تمام؟ ال .. الـ step to
+
+426
+00:30:33,270 --> 00:30:37,670
+caucus أجلكت هي hypurate hydrolysis positive، يعني
+
+427
+00:30:37,670 --> 00:30:39,430
+بتعمل hydrolysis لمين؟
+
+428
+00:31:00,230 --> 00:31:05,870
+هل هذه المعلومات الآن غريبة عليك؟ كلها مرت الآن
+
+429
+00:31:05,870 --> 00:31:10,190
+كلها مرت الآن أنا أحب دائمًا أقول Streptococcus
+
+430
+00:31:10,190 --> 00:31:13,910
+agalactia أو أي ميكروب أبدأه من الآخر
+
+431
+00:31:13,910 --> 00:31:16,270
+Streptococcus agalactia كيف عرفت أنه Streptococcus
+
+432
+00:31:16,270 --> 00:31:20,730
+agalactia لأنه كان test positive لأن الهيبيوريتا
+
+433
+00:31:20,730 --> 00:31:23,010
+هيدروليز test positive طب أنا ليش عملت أول فحصين
+
+434
+00:31:23,010 --> 00:31:25,910
+هدول لأنهم كانوا catalase negative طب أنا ليش
+
+435
+00:31:25,910 --> 00:31:28,670
+عملت فحصين catalase أساسًا لأنها gram positive
+
+436
+00:31:28,670 --> 00:31:33,610
+كوكسايل لأنها gram positive كوكسايل فعلية الصعود
+
+437
+00:31:33,610 --> 00:31:36,950
+من أسفل إلى أعلى أو من أقل إلى أسفل بحيث أنه
+
+438
+00:31:36,950 --> 00:31:40,610
+بالفعل يصير لنا نوع من الـ characterization وكلمة
+
+439
+00:31:40,610 --> 00:31:44,330
+characterization يعني identification و أحد أهم
+
+440
+00:31:44,330 --> 00:31:48,170
+نقاط عملنا
+
+441
+00:31:48,170 --> 00:31:52,190
+في الـ microbiology هو العزل والتعرف على الـ
+
+442
+00:31:52,190 --> 00:31:53,390
+microorganism
+
+443
+00:32:03,890 --> 00:32:08,170
+لأ لأ أنا بتكلم عن two organisms اللي نفترض أنه
+
+444
+00:32:08,170 --> 00:32:11,910
+بيعمله the same action killing the bacteria طبعًا
+
+445
+00:32:11,910 --> 00:32:14,550
+بيقتل البكتيريا زي الـ antibiotic زي الـ antibiotic,
+
+446
+00:32:15,090 --> 00:32:18,610
+antibiotic X ولنفترض أنه penicillin و antibiotic
+
+447
+00:32:18,610 --> 00:32:23,280
+Y ولنفترض أنه tetracycline هدول الاثنين لو حطيناهم
+
+448
+00:32:23,280 --> 00:32:27,960
+مع بعض تمام الـ tetracycline بيأثر على عمل مين
+
+449
+00:32:27,960 --> 00:32:32,100
+البنسلين فبدل ما واحد زائد واحد يساوي اثنين إيش
+
+450
+00:32:32,100 --> 00:32:37,200
+بيصير الآن واحد زائد واحد يساوي واحد لكن لو غيّرنا
+
+451
+00:32:37,200 --> 00:32:43,460
+التتراسيكلين وحطينا بداله gentamicin الجنتاميسين
+
+452
+00:32:43,460 --> 00:32:47,100
+بيتعاذر مع الـ penicillin وبدل ما واحد زاد واحد
+
+453
+00:32:47,100 --> 00:32:50,580
+يساوي اثنين بس واحد زاد واحد يساوي اثنين ثلاثة هذا
+
+454
+00:32:50,580 --> 00:32:54,100
+الكلام ننقله على مين؟ على الـ hemolysin hemolysin و
+
+455
+00:32:54,100 --> 00:32:58,840
+hemolysin زادوا من قدرة اللي هو واحد زاد من قدرة
+
+456
+00:32:58,840 --> 00:33:04,120
+الأخر وصلت؟ أنا
+
+457
+00:33:04,120 --> 00:33:07,760
+بدي أعطي مثال على الـ hemolysin هو عبارة عن إيش؟
+
+458
+00:33:07,760 --> 00:33:11,400
+عبارة عن توكسين الـ hemolysin عبارة عن توكسين ابدأي
+
+459
+00:33:11,400 --> 00:33:16,340
+بمثال واضح جدًا على two antibiotics اللي هم تأثير
+
+460
+00:33:16,340 --> 00:33:21,760
+تآذري وعبارة تعرف الـ pathway؟ إيش يعني pathway؟
+
+461
+00:33:21,760 --> 00:33:26,840
+يعني مسار metabolism، مسار metabolism المسار
+
+462
+00:33:26,840 --> 00:33:30,580
+الميتابوليزم عبارة عن خطوات واحد، اثنين، ثلاثة،
+
+463
+00:33:30,580 --> 00:33:35,680
+أربعة، خمسة واحد من الـ antibiotic بيؤثر، بيوجه
+
+464
+00:33:35,680 --> 00:33:41,520
+المسار عند النقطة هذه، تمام؟ وبي .. وبي .. وبي
+
+465
+00:33:41,520 --> 00:33:45,680
+.. وبيقتل البكتيريا لأنه بيوقف ال .. ال .. ال ..
+
+466
+00:33:45,680 --> 00:33:48,100
+ال metabolism عند النقطة هذه فما بتصيرش للنقطة
+
+467
+00:33:48,100 --> 00:33:52,640
+الأخيرة تمام؟ يجي Antibiotic ثاني استخدمناه بيوقف
+
+468
+00:33:52,640 --> 00:33:57,600
+النقطة هذه في المسار بيوقف النقطة الثانية صاروا
+
+469
+00:33:57,600 --> 00:34:01,100
+الاثنين بيشتغلوا على نفس المسار لكن على خطوات
+
+470
+00:34:01,100 --> 00:34:05,760
+مختلفة الأهم دلوقتي بتتخيل أنه عندي أنا الـ pathway
+
+471
+00:34:05,760 --> 00:34:09,080
+مكون من مجموعة من الـ enzymes والـ substrate صح ولا
+
+472
+00:34:09,080 --> 00:34:13,820
+لأ؟ واحد هرب من الـ antibiotic هذا لكن لن يهرب من
+
+473
+00:34:13,820 --> 00:34:16,980
+مين؟ من الـ antibiotic الثاني وصلت الصورة؟ بصير في
+
+474
+00:34:16,980 --> 00:34:20,240
+عندها في هذه الحالة اللي هو الـ action مضاعف أكثر من
+
+475
+00:34:20,240 --> 00:34:24,260
+الضعف تبع الانش يبقى بيشتغل على different steps في
+
+476
+00:34:24,260 --> 00:34:29,400
+pathway معين زي الـ toxin زي الـ hemolysis بقى طيب
+
+477
+00:34:30,440 --> 00:34:33,720
+عندي group D Streptococcus وهنفتن فيها محاضرتنا
+
+478
+00:34:33,720 --> 00:34:38,300
+اليوم group D Streptococcus من الـ microbat برضه
+
+479
+00:34:38,300 --> 00:34:42,160
+اللي احنا قلناه مهم يقولنا A وB وD مظبوط A B D
+
+480
+00:34:42,160 --> 00:34:46,400
+اخذنا A بيوجينز اخذنا B أجلاكتيا هتأخذ الآن بعض
+
+481
+00:34:46,400 --> 00:34:53,680
+المعلومات على group D أحد أهم مميزاتها، صفاتها، من
+
+482
+00:34:53,680 --> 00:34:57,720
+خلالها بممكن تتعرف عليها مقدرتها على النمو فيما
+
+483
+00:34:57,720 --> 00:35:04,420
+يسمى بالـ Bile esculin Agar الـ Bile esculin Agar هذا
+
+484
+00:35:04,420 --> 00:35:08,200
+ضامم صفتين في بعض، تعرفوا الـ Bile؟
+
+485
+00:35:11,520 --> 00:35:17,560
+أو صارت الصفراء فاكرين المقومكي كيف صار specific
+
+486
+00:35:17,560 --> 00:35:22,460
+أو selective؟ صار selective لأنه أضفنا عليه
+
+487
+00:35:22,460 --> 00:35:28,440
+crystal violet و bile source يبقى الـ bile القاتل
+
+488
+00:35:28,440 --> 00:35:33,220
+للجرام بوستر قاتل للجرام بوستر طفلات البكتيريه
+
+489
+00:35:33,220 --> 00:35:33,660
+للجرام بوستر
+
+490
+00:35:43,550 --> 00:35:48,710
+بالظبط وبالتالي صفتها صفتها من صفتها اللي عندها
+
+491
+00:35:48,710 --> 00:35:53,570
+المقدرة على تنمو في Bile في الـ Bile على عكس معظم الـ
+
+492
+00:35:53,570 --> 00:35:59,430
+gram-positive بكتيريا فهذا فحص مميز إليها، الآن هي
+
+493
+00:35:59,430 --> 00:36:02,970
+مش بس بتنمو في الـ Bile وعندها المقدرة على استغلال
+
+494
+00:36:02,970 --> 00:36:11,320
+مركب اسمه esculin فإذا البكتيريا نمت على الوصف هذا
+
+495
+00:36:11,320 --> 00:36:16,040
+فهي تمتلك الصفتين تمتلك صفة إنها قادرة إنها تنمو
+
+496
+00:36:16,040 --> 00:36:20,060
+في الـ Bile وقادرة تستغل من الـ esculin يعني مش صفة
+
+497
+00:36:20,060 --> 00:36:25,480
+واحدة صفتين وعندها المقدرة إنها تنمو في 6.5% NaCl
+
+498
+00:36:25,480 --> 00:36:33,500
+إذا نمت في الـ 6.5% فهي Enterococcus إذا نمتش في الـ
+
+499
+00:36:33,500 --> 00:36:37,280
+.. في الستة ونص فهي non-Enterococcus إذا الـ group
+
+500
+00:36:37,280 --> 00:36:41,280
+D Streptococcus الآن انقسمت لجسمين مين انقسمت
+
+501
+00:36:41,280 --> 00:36:45,940
+لجسمين؟ مقدرة البكتيريا على النمو في ستة و نصف 
+
+502
+00:36:45,940 --> 00:36:50,260
+percent sodium chloride ليكون إذا نمت فهي
+
+503
+00:36:50,260 --> 00:36:54,760
+enterococcus وإذا ما نمت فهي non-enterococcus إذا 
+
+504
+00:36:54,760 --> 00:36:57,180
+الـ group دي stripped cocaine فيها .. فيها نوعين
+
+505
+00:36:57,180 --> 00:37:01,290
+enterococcus و non-enterococcusو ايه المهمة الـ
+
+506
+00:37:01,290 --> 00:37:06,950
+Enterococcus اكتبوا عنكوا على لوحة بيبقاتكوا بالخط
+
+507
+00:37:06,950 --> 00:37:12,610
+العريض VRE Vancomycin 
+
+508
+00:37:12,610 --> 00:37:21,490
+Resistant Enterococci VRE V R E Vancomycin
+
+509
+00:37:21,490 --> 00:37:28,290
+Resistant Enterococci اسمه ما أعرفش مهمة المعلومة هي
+
+510
+00:37:28,290 --> 00:37:33,600
+VRE أنتوا بتعرفوا إن احنا تاريخنا عن الـ antibiotic
+
+511
+00:37:33,600 --> 00:37:39,180
+تاريخ طويل صار له تقريبا يعني من الأربعينيات ستين و
+
+512
+00:37:39,180 --> 00:37:44,100
+عشرين يعني تقريبا ثمانين سنة ثمانين سنة هذا
+
+513
+00:37:44,100 --> 00:37:50,360
+التاريخ قرّفه لطباء بمصطلح عصر المضادات الحيوية
+
+514
+00:37:50,360 --> 00:37:53,660
+تخيلوا إن احنا في يوم من أيام نسمي فترة زمنية
+
+515
+00:37:53,660 --> 00:37:58,480
+معينة بالمضادات الحيوية عصر المضادات الحيوية طب
+
+516
+00:37:58,480 --> 00:38:02,000
+علشان أسئلة و ليش أهميتها؟ احنا قبل شهر كنا نتكلم
+
+517
+00:38:02,000 --> 00:38:04,800
+عن الـ puripral sepsis و كثير من الأمهات كانت موت
+
+518
+00:38:04,800 --> 00:38:10,440
+من حمى نفس لكن مع وجود الـ antibiotics قلت فبالتالي
+
+519
+00:38:10,440 --> 00:38:13,380
+الـ antibiotics بالفعل أحدثت ثورة كبيرة في العلاج و
+
+520
+00:38:13,380 --> 00:38:17,900
+في الـ prevention الآن الـ antibiotics كانت رصيد مهم
+
+521
+00:38:17,900 --> 00:38:21,400
+جدا للأسف قصي استغلال و صار استخدامه في مجالات
+
+522
+00:38:21,400 --> 00:38:26,080
+متنوعة في تغذية الدواجن في علاج للحيوانات وبالتالي
+
+523
+00:38:26,080 --> 00:38:30,820
+بدأت البكتيريا يتكون له resistance الـ vancomycin أحد
+
+524
+00:38:30,820 --> 00:38:34,380
+أهم الـ antibiotics اللي كنا حاطينهم على الجهة
+
+525
+00:38:34,380 --> 00:38:39,950
+حاطينهم على العوزة تمام؟ ليش؟ لأنه بدأت الـ gram
+
+526
+00:38:39,950 --> 00:38:42,750
+-positive بكتيريا تكون resistant للبنسلينات والـ
+
+527
+00:38:42,750 --> 00:38:46,350
+cephalosporins و الـ vancomycin is very effective
+
+528
+00:38:46,350 --> 00:38:51,690
+against gram-positive بكتيريا فوصفته الـ WHO بالـ
+
+529
+00:38:51,690 --> 00:38:58,350
+vancomycin إنه آخر رصاصة آخر رصاصة في ترسانتها ضد
+
+530
+00:38:58,350 --> 00:39:01,830
+الـ gram-positive بكتيريا في فترة من الفترات لاحظوا
+
+531
+00:39:01,830 --> 00:39:08,990
+الوصف، وصف دقيق فلقة أو رصاصة و ترسانة عسكرية لأن
+
+532
+00:39:08,990 --> 00:39:14,530
+ما يدور بيننا وبين الميكروبات هو حقا حالة حرب. الآن
+
+533
+00:39:14,530 --> 00:39:20,630
+تخيلوا في غزة بدأنا نعزل كميات ضخمة بنسب مول يعني
+
+534
+00:39:20,630 --> 00:39:23,890
+يمكن قبل الـ 2008 .. 2008 ما بطلتش
+
+535
+00:39:23,890 --> 00:39:26,710
+اللي احنا كم سنة، نحن ست سنوات، يعني قبل ست سنوات
+
+536
+00:39:26,710 --> 00:39:31,730
+عزلنا ما نسبته .. ما نسبته أربعين في المئة من الـ
+
+537
+00:39:31,730 --> 00:39:35,270
+enterococci في المستشفى كانت resistant للـ
+
+538
+00:39:35,270 --> 00:39:41,210
+Vancomycin، بتوقع اليوم الوضع أصعب، في البحر بنعمل
+
+539
+00:39:41,210 --> 00:39:45,210
+دراسة حالية برضه نسبة والله يمكن 60% لحد الآن
+
+540
+00:39:45,210 --> 00:39:47,610
+للأرقام اللي .. اللي .. اللي .. اللي لحد الآن
+
+541
+00:39:47,610 --> 00:39:51,650
+تتوفر هنا 60% من العزلات تقريبا resistant لـ
+
+542
+00:39:51,650 --> 00:39:57,160
+الـ entero .. للعفو .. للـ vancomycin لدينا مشكلة
+
+543
+00:39:57,160 --> 00:40:00,680
+حقيقية لما أنا أقول VRA .. VRA ما أنا أحكي إن
+
+544
+00:40:00,680 --> 00:40:04,140
+الطلقة الأخيرة أو الرصاصة الأخيرة اللي كنا راحت
+
+545
+00:40:04,140 --> 00:40:08,840
+فبالتالي هتضطر نلجأ لـ extreme measures و ندفع فلوس
+
+546
+00:40:08,840 --> 00:40:11,780
+تخبرنا إن إنتهى الـ new antibiotics اللي هي مش
+
+547
+00:40:11,780 --> 00:40:15,040
+بالسهل أصبحنا كل عشر سنوات لما نكتشف إن الـ
+
+548
+00:40:15,040 --> 00:40:16,900
+antibiotic مناسب
+
+549
+00:40:26,310 --> 00:40:28,010
+و نكمل المرة القادمة إن شاء الله
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/VIGilQ1fnrY.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/VIGilQ1fnrY.srt
new file mode 100644
index 0000000000000000000000000000000000000000..45a91add4838faa0e14806b9b3a55ca1de15abf0
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/VIGilQ1fnrY.srt
@@ -0,0 +1,2576 @@
+1
+00:00:20,940 --> 00:00:22,640
+بسم الله الرحمن الرحيم الحمد لله رب العالمين
+
+2
+00:00:22,640 --> 00:00:25,780
+والصلاة والسلام وعلى سيدنا محمد وعلى آله وصحبه ومن
+
+3
+00:00:25,780 --> 00:00:30,500
+ساعة دربه اليوم الدين هذا يوم ختم محاضرتنا إن شاء 
+
+4
+00:00:30,500 --> 00:00:34,020
+الله نرجو من الله أن يكون كان فصل دراسي مثمر
+
+5
+00:00:34,020 --> 00:00:39,220
+واستفدنا منه معلومات تؤهلنا إلى المرحلة الثانية
+
+6
+00:00:39,220 --> 00:00:43,020
+التي هي مرحلة diagnostic microbiology اليوم هنكمل
+
+7
+00:00:43,020 --> 00:00:48,800
+بجزئية اسمها viral replication أنا أبتديت أن نختم
+
+8
+00:00:48,800 --> 00:00:56,860
+فيها لأنها مدخل لمفاهيم كثيرة صحيح ستزيد من
+
+9
+00:00:56,860 --> 00:01:00,180
+مداركنا إن شاء الله وقصة منها وتؤهلنا في مساعدة
+
+10
+00:01:00,180 --> 00:01:04,340
+الفيروسات إلى يعني أن نكون عندنا معلومات أساسية
+
+11
+00:01:04,340 --> 00:01:08,760
+رائعة جداً نبدأ بها course الفيروسات أيضاً بانطلاقة
+
+12
+00:01:08,760 --> 00:01:13,420
+جيدة إن شاء الله وأذكركم أن الفيروسات كانت عبارة
+
+13
+00:01:13,420 --> 00:01:17,200
+عن obligate intracellular parasites وبالتالي يعني
+
+14
+00:01:17,200 --> 00:01:21,290
+they cannot replicate independently، المستحيل يعني
+
+15
+00:01:21,290 --> 00:01:25,250
+يتكاثروا outside، كلمة independently يعني بشكل
+
+16
+00:01:25,250 --> 00:01:30,130
+مستقل وبالتالي they have to have a host to 
+
+17
+00:01:30,130 --> 00:01:33,790
+replicate within، ففي بعض الفيروسات تتخذ
+
+18
+00:01:33,790 --> 00:01:37,210
+البكتيريا host ومسمّى bacteria، وفيه قد تتخذ
+
+19
+00:01:37,210 --> 00:01:39,930
+النباتات ومسمّى plant viruses، أو قد تتخذ
+
+20
+00:01:39,930 --> 00:01:43,410
+الحيوانات وبمعنى animal viruses، أو قد تتخذ
+
+21
+00:01:43,410 --> 00:01:48,310
+الإنسان ومسمّى human viruses، their replication is
+
+22
+00:01:48,310 --> 00:01:50,570
+not by simple binary fission زي ما روحناها في
+
+23
+00:01:50,570 --> 00:01:54,130
+البكتيريا ولا mitosis ولا meiosis، one virus يدخل
+
+24
+00:01:54,130 --> 00:01:56,830
+داخل الخلية يطلع مئات من الـ virus، يطلع مئات
+
+25
+00:01:56,830 --> 00:02:02,650
+من الفيروسات، إذن سنحاول أن نطلع على الـ growth والـ
+
+26
+00:02:02,650 --> 00:02:08,630
+multiplication تبع الفيروس بصورتين شكلين، العدد مهم
+
+27
+00:02:10,070 --> 00:02:13,130
+والعيب، ممكن برضه ننظر إيه لو من .. فاكرين الـ
+
+28
+00:02:13,130 --> 00:02:19,430
+growth curve تبع البكتيريا؟ لو نظرنا لهذه النظرة
+
+29
+00:02:19,430 --> 00:02:24,270
+سنشوفه بنظرة مختلفة شوية عن البكتيريا، لكن النظرة
+
+30
+00:02:24,270 --> 00:02:28,810
+التي نحن سنبصل فيها أكثر هي stepwise، خطوة خطوة
+
+31
+00:02:28,810 --> 00:02:32,890
+كيف الفيروس من ما حدث له يجي خارج الخلية، ماذا يعني
+
+32
+00:02:32,890 --> 00:02:33,550
+بالتصفية في هذه الخلية؟
+
+33
+00:02:37,130 --> 00:02:40,270
+الفيروسات كلها تدخل بنفس الطريقة أم في اختلافات
+
+34
+00:02:40,270 --> 00:02:44,030
+لما يدخل ماذا يحدث؟ كيف .. وأين تبقى التكارث
+
+35
+00:02:44,030 --> 00:02:47,030
+في site of plasma أم في النواة؟ ما نوع المادة
+
+36
+00:02:47,030 --> 00:02:51,030
+الوراثية للفيروس؟ وهل نوع المادة الوراثية للفيروس
+
+37
+00:02:51,030 --> 00:02:54,050
+يؤثر على أن يكون الـ replication أو في site of
+
+38
+00:02:54,050 --> 00:02:58,270
+plasma أو في النواة؟ و .. و .. وكنت؟ وما هي الـ
+
+39
+00:02:58,270 --> 00:03:02,530
+replication ستبين معنا بـ stepwise، يعني خطوة ..
+
+40
+00:03:02,530 --> 00:03:07,770
+خطوة، نطلع على الشكل الذي أمامنا، هذا الشكل الذي أمامنا
+
+41
+00:03:07,770 --> 00:03:11,890
+يُبين لنا أنه تجاهز نتكلم عن زمن وعدد، هي عندي هنا
+
+42
+00:03:11,890 --> 00:03:17,470
+زمن صفر إلى 12 ساعة ونخطرها نتكلم عن hours، دخل
+
+43
+00:03:17,470 --> 00:03:20,870
+الفيروس one particle، هذا الـ number one particle
+
+44
+00:03:20,870 --> 00:03:26,170
+بعد ساعة ونصف تقريباً اختفى الفيروس لم يعد قابلاً
+
+45
+00:03:26,170 --> 00:03:30,490
+لأنه malodication، لماذا؟ لأنه دخل جوا الخلية وما
+
+46
+00:03:30,490 --> 00:03:36,010
+دخل جوا الخلية هو الآن بطل الفيروس كاملاً، وبالتالي
+
+47
+00:03:36,010 --> 00:03:42,070
+اختفى عن الوجود، بعد أربع ساعات إلى خمس ساعات صار
+
+48
+00:03:42,070 --> 00:03:47,670
+عندي خطين، خط المقطع هو المادة الوراثية، ممكن على
+
+49
+00:03:47,670 --> 00:03:52,070
+فكرة أن نكشف عن كمية المادة الوراثية، وممكن نكشف عن
+
+50
+00:03:52,070 --> 00:03:59,570
+عدد الفيروسات، واجبنا أن المادة الوراثية وعدد
+
+51
+00:03:59,570 --> 00:04:03,450
+الفيروسات ماشيين مع بعض بنفس الوسيلة، لكن لاحظوا
+
+52
+00:04:03,450 --> 00:04:08,010
+فينا logarithmic increase in number، برضه في زيادة
+
+53
+00:04:08,010 --> 00:04:12,410
+عددية مطردة كبيرة، بدأنا بالفيروس طلعنا على وين
+
+54
+00:04:12,410 --> 00:04:17,010
+وصلنا الآن للمستوى هنا 100 تقريباً أو أكثر من 100
+
+55
+00:04:17,010 --> 00:04:22,510
+أكثر من 100 خلال كم ساعة؟ هي من zero إلى 12 أو إلى 10
+
+56
+00:04:22,510 --> 00:04:26,810
+ساعات، 10 ساعات هي الـ maximum، هي الـ maximum طبعاً
+
+57
+00:04:26,810 --> 00:04:31,630
+هذا من خلية واحدة، لماذا إثبات هذا؟ لأن ما في خلايا
+
+58
+00:04:31,630 --> 00:04:35,290
+أخرى، ما في خلايا أخرى تعملها infection، ما في خلايا
+
+59
+00:04:35,290 --> 00:04:39,410
+أخرى تعملها .. يعني هو دخل في خلية واحدة وقصناها
+
+60
+00:04:39,410 --> 00:04:43,470
+لما طلع من نفس الخلية ما في خلايا أخرى، لو استمرنا
+
+61
+00:04:43,470 --> 00:04:47,590
+نوفر للفيروس خلايا، هلاجي أن الخط بضل بشكل إيه؟
+
+62
+00:04:47,590 --> 00:04:55,760
+مستقيماً، لحد ما يتنافس الخربان مع اللي هو الـ
+
+63
+00:04:55,760 --> 00:04:59,120
+effective أو الـ infective وبالتالي يعملة
+
+64
+00:04:59,120 --> 00:05:06,290
+neutralization، تصور عن الفيروس دخل اختفى بعدين فترة
+
+65
+00:05:06,290 --> 00:05:11,290
+اختفاء بيسموها فترة الكسوف eclipse، eclipse period
+
+66
+00:05:11,290 --> 00:05:17,490
+وفترة الزيادة بيسموها rise، اللي هي الصعود، صعود نجم
+
+67
+00:05:17,490 --> 00:05:20,850
+الفيروس في هذه الحالة، إذا في اختفاء وفي انهار صعود
+
+68
+00:05:20,850 --> 00:05:28,760
+الآن الفيروس لما دخل الخلية كيف طلع منها؟ طلع منها
+
+69
+00:05:28,760 --> 00:05:34,780
+بسلام خلها intact ولا نتجت الخلية اتدمر الخلية؟
+
+70
+00:05:34,780 --> 00:05:39,500
+هناك بعض الفيروسات عندها القدرة أن تحلل الخلية وهناك
+
+71
+00:05:39,500 --> 00:05:43,460
+فيروسات بتطلع من الخلية دون أن تؤذيها، دون أن
+
+72
+00:05:43,460 --> 00:05:47,560
+تؤذيها، إذن معنى الحكي أن في فيروسات بتدمر
+
+73
+00:05:47,560 --> 00:05:53,100
+الخلايا، أنا بتخيل معي طبقة من الخلايا، نوع من
+
+74
+00:05:53,100 --> 00:05:57,770
+أنواع الخلايا الطلائية لها شكل معروف تحت الـ
+
+75
+00:05:57,770 --> 00:06:02,370
+microscope normal cells ومونولير، طبقة واحدة، قدأ
+
+76
+00:06:02,370 --> 00:06:05,510
+حطينا فيها الفيروسات، الفيروس حط قدأ أعلى خلية
+
+77
+00:06:05,510 --> 00:06:09,390
+واحدة، دمرها، انتقل لخلايا أخرى، دمرها، إن أنا صار
+
+78
+00:06:09,390 --> 00:06:13,590
+في عندي abnormal morphology للخلايا، في destruction
+
+79
+00:06:13,590 --> 00:06:19,750
+في lysis أو الـ Change in the morphology، نوع
+
+80
+00:06:19,750 --> 00:06:23,170
+المورفولوجي بنسميه Cytopathic Effect اللي هو CPE
+
+81
+00:06:23,170 --> 00:06:28,030
+الذي مذكور قبلنا على الصورة CPE، Cytopathic Effect
+
+82
+00:06:28,030 --> 00:06:32,710
+لماذا أنا أهتم بها Cytopathic Effect؟ لأن كل فيروس
+
+83
+00:06:32,710 --> 00:06:38,210
+يعمل Cytopathic Effect خاص به، كلمة Cytopathic
+
+84
+00:06:38,210 --> 00:06:39,170
+Effect خاص به
+
+85
+00:06:42,110 --> 00:06:46,770
+في التعرف على من؟ على الفيروس طبعاً، كمان مرة أذكركم
+
+86
+00:06:46,770 --> 00:06:51,010
+not all viruses can produce cytopathic effect، لأن
+
+87
+00:06:51,010 --> 00:06:54,490
+نحن كنا جابين شوية، لأن في فيروسات أصلاً ما بتدمرش
+
+88
+00:06:54,490 --> 00:06:57,850
+الخلايا، وعشان أنا أؤكد على الحقيقة هذه بدي أرجع
+
+89
+00:06:57,850 --> 00:06:58,870
+أوريكم بعض الـ animation
+
+90
+00:07:18,930 --> 00:07:21,850
+الفيروس الآن المفروض أنه بيعمل عملية attachment
+
+91
+00:07:21,850 --> 00:07:26,610
+للخلية، بيعمل injection للـ DNA تبقى أو كله يدخل
+
+92
+00:07:26,610 --> 00:07:31,690
+داخل الخلية، وبعدين يبدأ عملية تكاثر وتصنيع
+
+93
+00:07:31,690 --> 00:07:35,070
+بروتينات، منها ما هو structural ومنها ما هو
+
+94
+00:07:35,070 --> 00:07:42,690
+enzymes، لغرض تشكيل كامل للفيروس، يعني أن المادة
+
+95
+00:07:42,690 --> 00:07:44,470
+الوراثية بشكل كبير
+
+96
+00:07:47,100 --> 00:07:51,780
+معرّنية بيطلع صيد بلازم بيصير له translation بيطلع
+
+97
+00:07:51,780 --> 00:07:54,200
+الـ protein ومن ضمنها الـ viral protein اللي هي الـ
+
+98
+00:07:54,200 --> 00:07:58,620
+capsid، بترجع وبتكون اللي هو الـ variant اللي هي
+
+99
+00:07:58,620 --> 00:08:03,420
+الفيروس الكامل، الآن بدي أعمل release، عمل release
+
+100
+00:08:03,420 --> 00:08:08,260
+عن طريق إيش؟ destruction للـ cell membrane، هذا الـ
+
+101
+00:08:08,260 --> 00:08:10,380
+destruction للـ cell membrane، كمان مرة
+
+102
+00:08:17,070 --> 00:08:19,810
+أنه characteristic يعني مميز للدعوة، لنطلع على
+
+103
+00:08:19,810 --> 00:08:26,330
+animation ثاني ونشوف
+
+104
+00:08:26,330 --> 00:08:29,330
+نوع آخر من الـ release لما يطلع الفيروس من بعد ..
+
+105
+00:08:29,330 --> 00:08:41,690
+من الـ .. من الخلية هذا
+
+106
+00:08:41,690 --> 00:08:45,290
+عمل إيش لنا؟ هذا عمل إيش اللي شفنا؟ اللي وقع على
+
+107
+00:08:45,290 --> 00:08:45,570
+طول
+
+108
+00:08:50,260 --> 00:08:55,580
+هلاجي أن الفيروس هو طالع مش هيعمل lysis، هيأخذ جزء
+
+109
+00:08:55,580 --> 00:08:59,140
+من الـ membrane ويطلع فيه، هذا سمينا إحنا body
+
+110
+00:08:59,140 --> 00:09:03,540
+عملية اللي هو الـ body، فمش كل الفيروسات عندها القدرة
+
+111
+00:09:03,540 --> 00:09:07,990
+تعمل cytopathic effect، طبعاً هو أكيد فيه ضرر للخلايا
+
+112
+00:09:07,990 --> 00:09:12,470
+لكن مش مرئي، مش visible، أنها غير قابلة أن نحن نشوفه
+
+113
+00:09:12,470 --> 00:09:16,650
+بطريقة واضحة، وأعتقد أن الفيروس هذا أو النموذج هذا
+
+114
+00:09:16,650 --> 00:09:22,070
+الذي قدمه الآن هو شكل على فيروس بيأخذ سيل من plane
+
+115
+00:09:22,070 --> 00:09:27,970
+بدون ما يعمل destruction للخلايا، بس يبدو أنه بيعمل
+
+116
+00:09:27,970 --> 00:09:32,130
+إثارة تشويق الفيروس هذا، فبالتالي بيأخذ وقت أطول في
+
+117
+00:09:32,130 --> 00:09:33,330
+العالم
+
+118
+00:09:35,820 --> 00:09:38,700
+هذه الـ glycoproteins خاصة بالفيروس، الفيروس اللي
+
+119
+00:09:38,700 --> 00:09:41,300
+بيصدرها، نعم هو الذي يكونها وهذا كل ما حكيناه في
+
+120
+00:09:41,300 --> 00:09:45,080
+المحاضرة
+
+121
+00:09:45,080 --> 00:09:50,300
+الأولى للفيروس هذا، أيوة دلّنا الـ cell membrane بقى
+
+122
+00:09:50,300 --> 00:09:56,040
+كمان، والخلية دلت عايشة، ودايماً ليس من الحكمة أن
+
+123
+00:09:56,040 --> 00:10:01,120
+الـ micro organism يقتل الـ host، قلنا لكم الميكروب
+
+124
+00:10:01,120 --> 00:10:07,480
+الناجح هو الذي بتكاثرُه يحدث أقل ضرر ممكن للـ hosts
+
+125
+00:10:07,480 --> 00:10:11,940
+لأن هذا يضمن اللي هو طالب الـ hosts survive، هو 
+
+126
+00:10:11,940 --> 00:10:18,000
+بينما قدر للـ hosts و بعدين راح يموت اللي هو
+
+127
+00:10:18,000 --> 00:10:20,400
+كل الـ virgin اللي طلعها الـ whites
+
+128
+00:10:28,790 --> 00:10:32,470
+تفضلنا أن هناك تصورين للـreplication، تصور
+
+129
+00:10:32,470 --> 00:10:35,070
+بالـgrowth curve و تصور stepwise اللي هو
+
+130
+00:10:35,070 --> 00:10:39,150
+خطوة بخطوة، يطلع على المجموع الخطوات الرئيسية في
+
+131
+00:10:39,150 --> 00:10:43,930
+تصنيع الفيروسة، أنا جالس هنا من أتلات مراحل، فاكرين؟
+
+132
+00:10:45,650 --> 00:10:50,270
+attachment and penetration أو entry, synthesis
+
+133
+00:10:50,270 --> 00:10:54,890
+وكلمة synthesis كانت كلمة واسعة تحتوي إلى معاملات
+
+134
+00:10:54,890 --> 00:10:58,730
+كبيرة، و في الآخر فين دي assembly و كان هنا release
+
+135
+00:10:58,730 --> 00:11:01,630
+لكن لاحظوا أن أنا فين دي خطوات أكثر من اللي أنا
+
+136
+00:11:01,630 --> 00:11:05,910
+حكيتهملأنه بالفعل عملنا عملية إيه؟ تفصيل في عملية
+
+137
+00:11:05,910 --> 00:11:10,050
+الـ synthesis، اتكلمنا عن early mRNA و early protein
+
+138
+00:11:10,050 --> 00:11:13,190
+synthesis، وبعدين اتكلمنا عن genome synthesis
+
+139
+00:11:13,190 --> 00:11:16,470
+وبعدين اتكلمنا عن late اللي هو protein mRNA و
+
+140
+00:11:16,470 --> 00:11:19,170
+protein synthesis، وبعدين اتكلمنا عن الـ assembly و
+
+141
+00:11:19,170 --> 00:11:22,910
+الـ release شكل
+
+142
+00:11:22,910 --> 00:11:27,330
+typical من أشهر الـ life cycles المعروفة الـ
+
+143
+00:11:27,330 --> 00:11:33,800
+adenovirus، الـ adenoviruses، طبعا الفيروس على الخلية
+
+144
+00:11:33,800 --> 00:11:37,600
+الخلية الكبيرة في هذه الدائرة هي الخلية الكبيرة
+
+145
+00:11:37,600 --> 00:11:40,460
+اللي في النص هي النوية، طبعا الآن الفيروس عمل
+
+146
+00:11:40,460 --> 00:11:46,160
+attachment، دخل الفيروس عمل uncoating، دخل المادة
+
+147
+00:11:46,160 --> 00:11:49,960
+الوراثية اللي هي شايفينها دي، لوين؟ للنوية، ليش دخل 
+
+148
+00:11:49,960 --> 00:11:54,460
+للنوية؟ لأن هو DNA virus، DNA virus، غالبا الـ DNA
+
+149
+00:11:54,460 --> 00:11:55,440
+virus بتحدم
+
+150
+00:11:59,870 --> 00:12:03,730
+على النوى، أول خطوة في النوى بصيله transcription هي
+
+151
+00:12:03,730 --> 00:12:07,870
+بتطلع منه mRNA، هاي الـ mRNA شايفينه؟ هذا هذا mRNA
+
+152
+00:12:07,870 --> 00:12:12,070
+بعد الـ mRNA مايتصنع، وان بروح على الـ cytoplasm
+
+153
+00:12:12,070 --> 00:12:16,010
+بتطلع، هي شايفينه؟ إيش بايش مرتبط عليه؟ مرتبط عليه الـ
+
+154
+00:12:16,010 --> 00:12:21,570
+ribosome، هنا يبقى صار لنا early protein synthesis
+
+155
+00:12:21,570 --> 00:12:26,330
+يبقى صار فاندي mRNA هي شايفينه؟ early mRNA، و بعدين
+
+156
+00:12:26,330 --> 00:12:26,750
+early
+
+157
+00:12:33,160 --> 00:12:38,320
+لأن البروتين تصنعت في الـ cytoplasm
+
+158
+00:12:41,110 --> 00:12:44,570
+ده أغلب النواة، هي ده أغلب النواة، يبقى معنا الحاجة
+
+159
+00:12:44,570 --> 00:12:48,950
+أن الـ early proteins لها دور رئيسي في تصنيع الـ
+
+160
+00:12:48,950 --> 00:12:53,210
+late protein و تصنيع الليبروجيني اللي هو المادة
+
+161
+00:12:53,210 --> 00:12:58,210
+الوراثية اللي هتشكل النسل دبع هذا الفيروس، و بنكمل
+
+162
+00:12:58,210 --> 00:13:02,050
+السلسلة، تصنية و تصنية و تصنية، و بالاخر، وسلسلة اللي
+
+163
+00:13:02,050 --> 00:13:07,010
+هو Tetris، إذن باختصار شديد جدا، Attachment,
+
+164
+00:13:07,170 --> 00:13:10,290
+Penetration, Uncoating, Early Synthesis للـ mRNA,
+
+165
+00:13:10,490 --> 00:13:13,710
+Early Synthesis للبروتين, Progeny DNA أو نوكليك
+
+166
+00:13:13,710 --> 00:13:17,690
+أسد، بعدين Late Synthesis للـ mRNA, Late Synthesis
+
+167
+00:13:17,690 --> 00:13:21,310
+للبروتين، بعدين Assembly، بعدين Release، نبدأ من
+
+168
+00:13:21,310 --> 00:13:25,630
+الآخر، من الآخر انتشرتوا اللي من الآخر، من الآخر
+
+169
+00:13:25,630 --> 00:13:29,350
+انتشرتوا قبل دقائق، الـ release، الـ release كان إن
+
+170
+00:13:29,350 --> 00:13:34,400
+عبر الـ lysis اللي هو الـ budding، عبر الـ budding اللي
+
+171
+00:13:34,400 --> 00:13:37,760
+بيعمل license، غالبا هذي استراتيجية من استراتيجيات
+
+172
+00:13:37,760 --> 00:13:43,920
+الفيروسات التي بتحتوي على envelope، بينما الـ
+
+173
+00:13:43,920 --> 00:13:47,600
+budding هو استراتيجية من استراتيجيات الفيروسات
+
+174
+00:13:47,600 --> 00:13:52,860
+اللي لها لـ envelope، طب الـ assembly كيف بيصير الـ
+
+175
+00:13:52,860 --> 00:13:59,460
+assembly؟ بيصير بطريقة نحن لم نفهمها بعد، يعني 
+
+176
+00:13:59,460 --> 00:14:04,600
+ماعندناش معلومات كافية عنها، اللي بنعرفه حاجة واحدة
+
+177
+00:14:04,600 --> 00:14:07,920
+ذكرناها في المحاضرة السابقة، الـ self-assembly
+
+178
+00:14:07,920 --> 00:14:11,900
+فاكرينها؟ و نقول لما جبنا purified protein و
+
+179
+00:14:11,900 --> 00:14:15,700
+جيبنا mRNA تبع الفيروس و حطناهم في test tube
+
+180
+00:14:15,700 --> 00:14:22,770
+فاجأنا لحالهم أنهم إيه؟ و هذا جونا عمليعني قريب من
+
+181
+00:14:22,770 --> 00:14:26,870
+المغناطيسات مثلا، يعني لو جيبت أنا مجموعة مغناطيسات
+
+182
+00:14:26,870 --> 00:14:29,950
+أحلتها مع بعض، بين اللي جيهم أخذوا شكل حالهم من غير
+
+183
+00:14:29,950 --> 00:14:32,190
+ما نبدل أي جهد، يلزجوا مع بعض، لا بدنا اللي لزجوا
+
+184
+00:14:32,190 --> 00:14:36,020
+ولا غير، و لزجوا مع بعض، يجي المشكلة أو أخذنا نقول
+
+185
+00:14:36,020 --> 00:14:40,620
+التفسير الأقرب إلى أنه .. نقول أن تركيب الـ
+
+186
+00:14:40,620 --> 00:14:43,640
+protein و المادة الوراثية في بينهم interaction ..
+
+187
+00:14:43,640 --> 00:14:49,480
+interaction تلقائي، لا يستلزم أي نوع من التدخل اللي
+
+188
+00:14:49,480 --> 00:14:54,010
+بتطاقة، الخلية مش هتصرف طاقة على الـ assembly، الـ
+
+189
+00:14:54,010 --> 00:14:59,450
+assembly عملية غالب الأمر self motivated، ناتجة عن
+
+190
+00:14:59,450 --> 00:15:02,910
+مين؟ ناتجة عن الـ nature of the structure of
+
+191
+00:15:02,910 --> 00:15:05,850
+protein and nucleic acid، بتفاعلوا مع بعض و
+
+192
+00:15:05,850 --> 00:15:09,750
+بيرتبطوا بطريقة نظامية معينة، أما كده بالضبط ماعندنا
+
+193
+00:15:09,750 --> 00:15:13,670
+فكرة الصحيح، خلاصها من الـ assembly، الـ assembly
+
+194
+00:15:13,670 --> 00:15:19,300
+غالبا بتتم .. غالبا بتتم في الخلية، وبعدين و الفيروس
+
+195
+00:15:19,300 --> 00:15:22,120
+طالع خلاص، يعني هي بيكون already جاهز في بعض
+
+196
+00:15:22,120 --> 00:15:26,180
+الفيروسات، الـ assembly بتتم في أخر لحظات قبل خروجه
+
+197
+00:15:26,180 --> 00:15:30,820
+بلحظات، شفت الـ budding؟ الـ budding عند عريض الـ
+
+198
+00:15:30,820 --> 00:15:34,520
+budding بيصير في اللي هو الـ assembly الأخيرة و
+
+199
+00:15:34,520 --> 00:15:37,100
+بيطلع، يعني الـ maturation للفيروس، الـ maturation
+
+200
+00:15:37,100 --> 00:15:41,320
+للفيروس، طب نرجع للخطوة اللي قبلها، كان في عندي أنا
+
+201
+00:15:41,320 --> 00:15:44,770
+protein synthesis صح؟ و ده protein synthesis، خدوا
+
+202
+00:15:44,770 --> 00:15:49,050
+بالكم، الـ protein synthase ممكن يتم بطريقتين، عندي
+
+203
+00:15:49,050 --> 00:15:52,490
+خيط طويل من الـ mRNA بيجري البزوم، بيجري من أول و
+
+204
+00:15:52,490 --> 00:15:57,870
+لآخر بيطلع عنده long polypeptide، مايله أي وظيفة
+
+205
+00:15:57,870 --> 00:16:02,710
+long polypeptide، مالوش أي وظيفة بالضبط، أنا بشبهها
+
+206
+00:16:02,710 --> 00:16:06,370
+قطعة الخشب عند النجار، بتكون محتوطة مايلها غرض و
+
+207
+00:16:06,370 --> 00:16:11,250
+ليها مسمى و ليها وظيفة، قطعة خشب فجأة بيبرسم عليها
+
+208
+00:16:11,250 --> 00:16:15,120
+و بجسها بالمنشار اللي صارت طاولة، صح لها function صح
+
+209
+00:16:15,120 --> 00:16:19,320
+لها في هذه الحالة functional على طرف الـ
+
+210
+00:16:19,320 --> 00:16:23,620
+polypeptide بيكون قطعة بيسموها protease، انزين
+
+211
+00:16:23,620 --> 00:16:28,080
+protease تفصل حالها من حالها من القطع هذه، و بتبدأ
+
+212
+00:16:28,080 --> 00:16:33,100
+تجتف الـ polypeptide من أماكن محددة، طبعا كل قطعة
+
+213
+00:16:33,100 --> 00:16:37,620
+تنتج بيسميها functional، functional polypeptide
+
+214
+00:16:39,250 --> 00:16:42,610
+أو functional protein، نشرت من إيه؟ من قطعة طويلة
+
+215
+00:16:42,610 --> 00:16:45,130
+بيسموها precursor polypeptide، precursor
+
+216
+00:16:45,130 --> 00:16:50,650
+polypeptide، إذا هناك فيروسات عندها استراتيجية أن
+
+217
+00:16:50,650 --> 00:16:55,130
+تصنع قطعة طويلة من الـ polypeptide، سمنها precursor
+
+218
+00:16:55,130 --> 00:16:59,690
+وبعدها إنزيم البروتياز هو اللي بيجتح، نأخذ فائدة من
+
+219
+00:16:59,690 --> 00:17:06,330
+هذا الموضوع، البروتياز بدونه .. اه بدونه الفيروس مش
+
+220
+00:17:06,330 --> 00:17:09,290
+هيقدر يكمل الـ life cycle تبعته، لأنه معتمد اعتماد
+
+221
+00:17:09,290 --> 00:17:12,090
+كامل عالمين على الـ protein أن يتقطع و يصبح
+
+222
+00:17:12,090 --> 00:17:17,390
+functional، فلو استخدمنا احنا drugs that will
+
+223
+00:17:17,390 --> 00:17:20,330
+inhibit the إنزيم اللي اسمه البروتياز، و بيسموه
+
+224
+00:17:20,330 --> 00:17:24,740
+بالفعل البروتياز inhibitors، ممكن نستهدف تلك
+
+225
+00:17:24,740 --> 00:17:30,260
+الفيروسات التي .. التي من استراتيجيتها أن تصنع
+
+226
+00:17:30,260 --> 00:17:33,580
+.. تصنع اللي هو قلنا الـ long poly peptide و من
+
+227
+00:17:33,580 --> 00:17:37,980
+أشهر الفيروسات كرمولة اللي بنستخدمها Anti Protease
+
+228
+00:17:37,980 --> 00:17:41,300
+Inhibitors أو Protease Inhibitors اللي هي الـ HIV
+
+229
+00:17:41,300 --> 00:17:47,780
+الـ HIV، وصلت النقطة، و هناك طبعا الفيروسات
+
+230
+00:17:54,800 --> 00:17:59,380
+بينما النوع الآخر من الفيروسات، النوع الآخر من
+
+231
+00:17:59,380 --> 00:18:03,820
+الفيروسات أصلا عنده المادة الوراثية مقطعة، زي الـ
+
+232
+00:18:03,820 --> 00:18:08,280
+influenza virus، الـ influenza virus، فكل قطعة بصي
+
+233
+00:18:08,280 --> 00:18:13,740
+لها transcription و بيمر عليها الـ translation و
+
+234
+00:18:13,740 --> 00:18:16,460
+بتاني بتنتج functional protein، ككل قطعة بتنتج
+
+235
+00:18:16,460 --> 00:18:19,560
+functional protein، يبقى في عندنا two strategies
+
+236
+00:18:19,560 --> 00:18:25,440
+كمان مرة واحدة و بشرة أن نعملها functional protein
+
+237
+00:18:25,440 --> 00:18:29,420
+و واحدة precursor polypeptide من خلالها بنصنع
+
+238
+00:18:29,420 --> 00:18:35,160
+مجموعة من البروتينات مرة واحدة، طب هذا متعلق بمين؟
+
+239
+00:18:35,160 --> 00:18:41,320
+متعلق بالـ polypeptide و الـ protein synthesis، طب
+
+240
+00:18:41,320 --> 00:18:43,880
+احنا جاب الـ protein synthesis فيها مشكلة كبيرة
+
+241
+00:18:43,880 --> 00:18:48,060
+اللي أهم من كل هذه تلقى عليك أنت الطريقة اللي
+
+242
+00:18:48,060 --> 00:18:52,400
+بيجيها من أي بواية بتيجيها؟ من البوارة الغربية
+
+243
+00:18:52,400 --> 00:18:57,020
+الشرقية، و فيه بيجوا من البوارة الجنوبية، و في بعض
+
+244
+00:18:57,020 --> 00:18:59,700
+الأحيان لك، لكن كلهم وان بيروحوا في الآخر بيجوا على
+
+245
+00:18:59,700 --> 00:19:03,840
+القاعة، بيجوا على القاعة، دخلوا من أبواب مختلفة
+
+246
+00:19:03,840 --> 00:19:06,260
+بطولة مختلفة، لكن في النهاية دخلوا من الأساس
+
+247
+00:19:06,260 --> 00:19:11,610
+المسلطة، احنا الفيروسات تشكلت، المادة اللي قاعدة
+
+248
+00:19:11,610 --> 00:19:14,650
+تبعتها بطرق مختلفة، فيه positive-stranded DNA، فيه
+
+249
+00:19:14,650 --> 00:19:17,830
+single-stranded DNA، فيه positive polarity single
+
+250
+00:19:17,830 --> 00:19:21,510
+-stranded، و فيه negative polarity single-stranded
+
+251
+00:19:21,510 --> 00:19:24,630
+RNA وفيه الـ double-stranded أساسا RNA يعني
+
+252
+00:19:24,630 --> 00:19:28,150
+استراتيجيات مختلفة في المادة الوراثية
+
+253
+00:19:42,890 --> 00:19:46,870
+هذه هي الـ ultimate، الـ goal
+
+254
+00:19:52,030 --> 00:19:57,330
+هو يترجم عشان يترجم لازم يتحول لـ mRNA طبعا هناك
+
+255
+00:19:57,330 --> 00:20:02,130
+فيروسات سهل جدا واصلا طبيعتها هي جاية positive
+
+256
+00:20:02,130 --> 00:20:06,330
+sense positive sense mRNA بتقول الريبوزوم تعال
+
+257
+00:20:06,330 --> 00:20:06,590
+اقرأ
+
+258
+00:20:12,550 --> 00:20:17,430
+هل احنا بطبيعة الخلايا بتاعتنا فهي لنا إمكانية
+
+259
+00:20:17,430 --> 00:20:20,390
+نحوّل الـ negative لـ positive خلايا ناحنا الـ host
+
+260
+00:20:20,390 --> 00:20:24,830
+يعني الفيروس بتكون جايب معاها إيه؟ الـ enzyme .. الـ
+
+261
+00:20:24,830 --> 00:20:28,730
+enzyme بتاعه .. الـ enzyme بتاعه هنا يجب أن نتوقف و
+
+262
+00:20:28,730 --> 00:20:35,930
+نتحدث عن concept مهم جدا الفيروس بيحتاج أن يكون
+
+263
+00:20:35,930 --> 00:20:42,120
+معاه enzyme خاص فيه وفيروس آخر لا يحتاج  للتانين
+
+264
+00:20:42,120 --> 00:20:47,060
+حطيتهم في .. في أنبوبتين وغسلتهم شيلت منهم
+
+265
+00:20:47,060 --> 00:20:51,840
+الكبسيد خالص وشيلت منهم أي بروتين إيش اللي طلع
+
+266
+00:20:51,840 --> 00:20:56,620
+معايا فقط نوكليك .. النوكليك أسيد أنا اللي قولنا
+
+267
+00:20:56,620 --> 00:20:59,800
+عنه بـ enzyme هذا أظن أنه آه آه وبتاني بدوش enzyme
+
+268
+00:20:59,800 --> 00:21:04,420
+اللي أنا أخدتهم أخدت مادة بلاتية فقط فضل حطهم على
+
+269
+00:21:04,420 --> 00:21:08,680
+الخلايا تفاجأنا أن هذا الفيروس عمل infection
+
+270
+00:21:08,680 --> 00:21:14,370
+للخلايا وطلعنا فيروسها وهذا فاش أطلقنا عليه لقب،
+
+271
+00:21:14,370 --> 00:21:18,810
+بيقول إن هذا اسمه infectious nucleic acid وهذا non
+
+272
+00:21:18,810 --> 00:21:23,730
+-infectious قاعدة كل فيروس يحتاج إن الانزيم بتاعه
+
+273
+00:21:23,730 --> 00:21:27,750
+يكون معاه فهو non-infectious بينما كل فيروس
+
+274
+00:21:27,750 --> 00:21:32,710
+ما بيحتاج إن زهر يكون معاه فهو قادر أن يعمل
+
+275
+00:21:32,710 --> 00:21:37,630
+infectious طب تعالوا ناخد حكمة من الموضوع مين اللي
+
+276
+00:21:37,630 --> 00:21:42,530
+كان سبب في إنه الفيروس يصيب X وما يصيبش Y؟ وجود
+
+277
+00:21:42,530 --> 00:21:45,570
+الـReceptors؟ لأ، أتكلم عن الـ .. عن الفيروس بشكل
+
+278
+00:21:45,570 --> 00:21:47,750
+عام، اللي أنا خلصت سنة من الفكرة هذه، رجعنا
+
+279
+00:21:47,750 --> 00:21:53,290
+للفيروس الأب، العادي، من المسئول عن إنه يصيب X وما 
+
+280
+00:21:53,290 --> 00:21:56,530
+يصيبش Y هو الـReceptors والـAnti-Radين المولودة
+
+281
+00:21:56,530 --> 00:21:59,730
+عليه؟ طب لما أنا أخدت المادة الوراثية، المادة
+
+282
+00:21:59,730 --> 00:22:04,990
+الوراثية إيه الـReceptors؟ بينجأ أنا تخطيت حاجز
+
+283
+00:22:04,990 --> 00:22:10,200
+اسمه Specificity صح هذا لأ بجد بالأول مضطر أن عشان
+
+284
+00:22:10,200 --> 00:22:13,700
+هذا الفيروس يدخل داخل الخلية إيش أعمل؟ أحط لازم
+
+285
+00:22:13,700 --> 00:22:16,860
+يكون له receptor طب هذا الآن أنا بتكلم عن مادة
+
+286
+00:22:16,860 --> 00:22:20,120
+وراثية مش محتاجة receptor هتدخل إلى داخل الخلية
+
+287
+00:22:20,120 --> 00:22:26,920
+smoothly وانتهى الموضوع لكن سؤال دخلناه داخل
+
+288
+00:22:26,920 --> 00:22:33,240
+الخلية وتكاثر الفيروس لكن خدوا بالكم الفيروس دمر
+
+289
+00:22:33,240 --> 00:22:37,830
+الخلية اللي اتكاثر فيها والآن طلع على خلايا أخرى هل
+
+290
+00:22:37,830 --> 00:22:41,590
+بيقدر يعملها infection؟ آه بيقدر يعملها في حالة
+
+291
+00:22:41,590 --> 00:22:45,210
+واحدة أنه .. أنه لها receptor بس إذا من هات الـ
+
+292
+00:22:45,210 --> 00:22:50,730
+receptor .. يعني أعطيكم مثال صارخ وبقب أسماء،
+
+293
+00:22:50,730 --> 00:22:54,730
+فيروس الـ poliovirus .. الـ poliovirus بيصيب مثلا
+
+294
+00:22:54,730 --> 00:22:58,950
+الإنسان والـ .. الـ .. وبعض المخلوقات الشبيهة اللي
+
+295
+00:22:58,950 --> 00:23:01,790
+زي القرد يعني الـ higher .. يسموه higher animals،
+
+296
+00:23:01,790 --> 00:23:06,690
+اتبعين؟ لكن ما بيصيبش الزواحف لو أنا أخدت الـ virus بتاع
+
+297
+00:23:06,690 --> 00:23:10,390
+العفن، المادة الوراثية وحقنتها بالزواحف، هيعمل
+
+298
+00:23:10,390 --> 00:23:15,110
+infection، لكن الخلية اللي هي حقنتها فيها، هيطلع
+
+299
+00:23:15,110 --> 00:23:18,250
+الـ virus مش هيجي خلايا أخرى يقدر يصيبها، لكن في
+
+300
+00:23:18,250 --> 00:23:22,350
+هذه الفكرة أنه ثبت أنه نقدر نتخطى حاجز الـ
+
+301
+00:23:22,350 --> 00:23:27,490
+specificity في هذا الحد، في هذا المعنى نرجع نبرجها
+
+302
+00:23:27,490 --> 00:23:31,190
+إيش اللي خلانا نتكلم عنها infectious وnon-infectious
+
+303
+00:23:31,190 --> 00:23:37,110
+طيب الفيروس اللي الـ DNA double-stranded و single
+
+304
+00:23:37,110 --> 00:23:41,070
+-stranded positive أو single-stranded negative كيف
+
+305
+00:23:41,070 --> 00:23:44,650
+كل المدن اللي بتتصرفوا داخل الخلايا هنحاول نتعرف
+
+306
+00:23:44,650 --> 00:23:48,050
+عليهم عنهم لكن لأ بقصة من المرور سريع الكلام اللي
+
+307
+00:23:48,050 --> 00:23:53,810
+حاكينها الـ attachment هي اللي بتحدد specificity والـ
+
+308
+00:23:53,810 --> 00:23:57,430
+host range بيصيب مين وكيف وحتى الـ organ
+
+309
+00:23:57,430 --> 00:24:02,330
+specificity اللي ذاكرة قالها هذه مهمة جدا لإنه
+
+310
+00:24:02,330 --> 00:24:09,010
+بنلاقي إنه liver viruses زي مثلا hepatitis viruses
+
+311
+00:24:10,170 --> 00:24:14,590
+هي سميت hepatitis virus مش لإنه عيلة واحدة، هم من
+
+312
+00:24:14,590 --> 00:24:18,670
+عائلات مختلفة، لكن الـ primary target بتاعهم هو الـ
+
+313
+00:24:18,670 --> 00:24:23,270
+liver cells، يعني hepatitis A مختلفة عن B عن C عن
+
+314
+00:24:23,270 --> 00:24:27,030
+D، كل المختلفين عن بعض، لكن الشيء المشترك بينهم
+
+315
+00:24:27,030 --> 00:24:30,450
+إنهم بيستهدفوا الـ liver cells، يبقى organ
+
+316
+00:24:30,450 --> 00:24:32,650
+specificity في هذه الحالة
+
+317
+00:24:35,140 --> 00:24:40,180
+هل لما الفيروس بيرتبط على الـ receptor عندنا، هل هذا
+
+318
+00:24:40,180 --> 00:24:44,880
+الـ receptor مخصص، مصمم، عشان يستقبل الفيروس؟ لأ،
+
+319
+00:24:44,880 --> 00:24:48,260
+مش معقول، بس اللي معقول، طبعا، فهذه مثلًا، مثلًا
+
+320
+00:24:48,260 --> 00:24:52,200
+خلافية، هنتناقش فيها يوم من الأيام، لكن اليوم بدنا
+
+321
+00:24:52,200 --> 00:24:55,920
+نعرف أنه، احنا بدنا نعرف أنه في بعض الفيروسات
+
+322
+00:24:55,920 --> 00:25:00,300
+بيرتبطوا بـ receptors، هذه الـ receptors اللي لها non
+
+323
+00:25:00,300 --> 00:25:05,820
+-function، يعني لوظيفة معروفة، مثلًا الـ CD4 اللي
+
+324
+00:25:05,820 --> 00:25:10,040
+بيرتبط عليها اللي هو الـ HIV هي الـ normal function
+
+325
+00:25:10,040 --> 00:25:14,820
+بتاعتها أنها ترتبط لـ class II MHC و class II MHC
+
+326
+00:25:14,820 --> 00:25:19,020
+بروتين مهم جدا في عملية تنشيط الـ T-Helper cell
+
+327
+00:25:19,020 --> 00:25:22,540
+يجه هو مش مصمم بالفيروس بس أنا بقوله كيف أصيب
+
+328
+00:25:22,540 --> 00:25:28,680
+الموضوع أنا الغرفة يادي المفتاح عشان ندخل
+
+329
+00:25:34,320 --> 00:25:38,320
+تمام؟ يبقى فيه Normal Function للـ Receptors لكن
+
+330
+00:25:38,320 --> 00:25:43,260
+الفيروسات استطاعت أن تمتلك مفاتيح للارتباط بهذه
+
+331
+00:25:43,260 --> 00:25:47,240
+الـ Receptors وعن طريقها استطاعت أن تستهدف طبعا هل
+
+332
+00:25:47,240 --> 00:25:50,500
+هذا متعمد ولا غير متعمد؟ هذا في علم الغيب، ربنا
+
+333
+00:25:50,500 --> 00:25:53,460
+سبحانه وتعالى هو اللي خلقه للمخلوقات ويا ذالري إن
+
+334
+00:25:53,460 --> 00:25:57,860
+كان هناك حكمة، أكيد حكمة، هناك حكمة، لكن لا العلم
+
+335
+00:25:57,860 --> 00:26:02,040
+هنا الـ Rabies Virus بيرتبط بالـ Acetylcholine
+
+336
+00:26:02,040 --> 00:26:05,680
+Receptor إصدار كلين Receptors عشان هيك بيصيب فيانا
+
+337
+00:26:05,680 --> 00:26:10,120
+أمراض متعلقة بالعصب في الـ .. ده الكلام الـ Epstein
+
+338
+00:26:10,120 --> 00:26:12,040
+-Barr أو الـ Epstein-Barr Virus بيرتبط لـ الـ
+
+339
+00:26:12,040 --> 00:26:16,400
+complement receptor الـ Vaccinia virus بيرتبط بحدث
+
+340
+00:26:16,400 --> 00:26:18,760
+يسمى epidermal growth factor receptor
+
+341
+00:26:21,790 --> 00:26:24,890
+وهو الـ M1 اللي هو Intracellular Adhesin Molecule
+
+342
+00:26:24,890 --> 00:26:29,710
+وهذا برضه عبارة معروف باسم CD وكلمة CD معرفت مرة
+
+343
+00:26:29,710 --> 00:26:32,610
+معاهم قبل البداية CD معناها cluster of
+
+344
+00:26:32,610 --> 00:26:35,630
+differentiation وهو عبارة عن أنتيجين بيستخدموه
+
+345
+00:26:35,630 --> 00:26:39,170
+لتمييز بين خلايا الـ white blood cell
+
+346
+00:26:43,480 --> 00:26:46,160
+بالنسبة للـ Penetration، بالنسبة للـ Penetration،
+
+347
+00:26:46,160 --> 00:26:49,960
+فاكرين المحاضرة السابقة كانت بتتكلم عن Endocytosis
+
+348
+00:26:49,960 --> 00:26:54,400
+أو عن Fusion، تمام عن Fusion، وبعض الأحيان فقط
+
+349
+00:26:54,400 --> 00:27:01,260
+Injection للمادة الوراثية الـ Uncoating جوا هنا من
+
+350
+00:27:01,260 --> 00:27:05,680
+الـ pH المنخفض للـ physical اللي فيها الفيروس،
+
+351
+00:27:05,680 --> 00:27:13,100
+favors Uncoating بتستغربوا احنا كنا بنقول إنه يعني
+
+352
+00:27:13,100 --> 00:27:18,540
+مثلًا في أشياء زي الأجار بتكون صلبة بنسخنها تسعة
+
+353
+00:27:18,540 --> 00:27:21,720
+وتمانين وتسعين بتكون لسه صلبة تسعة وتسعين بتصير
+
+354
+00:27:21,720 --> 00:27:26,740
+سائلة، مظبوط؟ درجة حرارة واحدة بتغير من طبيعات
+
+355
+00:27:26,740 --> 00:27:33,060
+المركب فالـ bonding بتاع الـ capsomers المكونة للكبسيد
+
+356
+00:27:33,060 --> 00:27:37,280
+بيقول لنا بالـ pH المنخفض يعني بتتفكك، الروابط هذه
+
+357
+00:27:37,280 --> 00:27:41,200
+اللي هي الضعيفة، الروابط الضعيفة بتتفكك وبالتالي بـ
+
+358
+00:27:41,200 --> 00:27:44,000
+circuit release للمادة إيه والعادي، إذا الـ low pH
+
+359
+00:27:44,000 --> 00:27:49,920
+في الـ physical favors عن كوترال bacteriophage طبعا
+
+360
+00:27:49,920 --> 00:27:54,040
+فاكرينه آه بيعمل عملية Injection وإذا إنتوا
+
+361
+00:27:54,040 --> 00:27:59,180
+بتشوفوا أجاز من أروع ما يكون دور على اليوتيوب على
+
+362
+00:27:59,180 --> 00:28:04,420
+الـ penetration للـ bacteriophage للـ bacterial cell
+
+363
+00:28:04,420 --> 00:28:08,460
+بتشوفوا الـ contracting sheet تمام كيف بتعمل
+
+364
+00:28:08,460 --> 00:28:12,140
+Injection للمادة اللي ورا دي كيف الـ base اللي
+
+365
+00:28:12,140 --> 00:28:15,820
+موجودة في الـ core هنا في القطع هذه شايفينها بتحتوي
+
+366
+00:28:15,820 --> 00:28:21,300
+أصلًا على أنظمة ما اللي عايزنا نسميها Injection فالـ ..
+
+367
+00:28:21,300 --> 00:28:25,300
+الـ .. الـ .. الـ replication للبكتيريا اللي شفناها
+
+368
+00:28:25,300 --> 00:28:28,640
+احنا فاكتر من المناسبة وممكن نركزنا عليه أكثر
+
+369
+00:28:28,640 --> 00:28:32,420
+حاليًا لما تكلمنا عن الـ transduction إيه طبعا
+
+370
+00:28:32,420 --> 00:28:37,780
+animated GIF كرتون يعني بيوضح لنا بشكل مبسط بصريا
+
+371
+00:28:37,780 --> 00:28:41,920
+كيفية اللي هو الدخول إلى البكتيريا لحظة إنه
+
+372
+00:28:44,650 --> 00:28:48,230
+فيه احتكاك مع الـ cell wall فيه Injection للمادة
+
+373
+00:28:48,230 --> 00:28:52,990
+الوراثية في سيطرة كاملة على الخلية وتصنيع ومن ثم
+
+374
+00:28:52,990 --> 00:28:59,090
+عملية تجميع و release by lysis هذا نوع من الـ lysis
+
+375
+00:28:59,090 --> 00:29:03,550
+effect إذا 
+
+376
+00:29:03,550 --> 00:29:05,370
+نقدر نقول إنها غلطية للجزء الأول اللي هو
+
+377
+00:29:05,370 --> 00:29:07,910
+الattachment والpenetration والuncoating كلها
+
+378
+00:29:07,910 --> 00:29:10,290
+لأنها عن ال early viral mRNA synthesis والearly 
+
+379
+00:29:10,290 --> 00:29:11,630
+protein synthesis
+
+380
+00:29:14,360 --> 00:29:20,140
+في عاملين مهمين جدا بيأثروا على رؤيتنا لكيفية ال
+
+381
+00:29:20,140 --> 00:29:23,380
+replication العامل الأول المادة الوراثية إيش 
+
+382
+00:29:23,380 --> 00:29:28,660
+طبيعتها؟ رقم اثنين أين يتكاثر؟ في النواة ولا في
+
+383
+00:29:28,660 --> 00:29:34,520
+الـcytoplasm؟ أكثر صفحة محتوية على معلومات بدنا 
+
+384
+00:29:34,520 --> 00:29:38,620
+نبقى مركزين عليها طول الوقت تمام؟ ونشوف إيش الفرق
+
+385
+00:29:38,620 --> 00:29:44,930
+ما بين ال DNA virus وال RNA virus لاحظوا كلهم ال
+
+386
+00:29:44,930 --> 00:29:49,450
+DNA viruses بيتكونوا من double-stranded DNA ماعدا 
+
+387
+00:29:49,450 --> 00:29:53,310
+الparvo virus يبقى فيه exception واحد اسمه parvo
+
+388
+00:29:53,310 --> 00:29:59,430
+virus هو single-stranded DNA they all replicate in
+
+389
+00:29:59,430 --> 00:30:06,610
+the nucleus ماعدا ال parvo viruses يبقى كلهم بتكاثروا
+
+390
+00:30:06,610 --> 00:30:10,390
+في النواة ماعدا ال parvo virus اللي هو الجملة الأخيرة
+
+391
+00:30:11,280 --> 00:30:15,360
+They use
+
+392
+00:30:15,360 --> 00:30:20,160
+a host cell DNA dependent RNA polymerase to
+
+393
+00:30:20,160 --> 00:30:24,080
+synthesize their mRNA. Are they infectious or non
+
+394
+00:30:24,080 --> 00:30:24,940
+-infectious؟
+
+395
+00:30:28,300 --> 00:30:33,360
+ال DNA تبعهم لأ اللي جاب ال DNA Virus اللي حتى 
+
+396
+00:30:33,360 --> 00:30:36,460
+اللي بتكلم عنهم اللي هم بيستخدموا ال DNA dependent
+
+397
+00:30:36,460 --> 00:30:39,740
+RNA Polymerase لبعض الخلية لأن هو بيستخدموش معاهم
+
+398
+00:30:39,740 --> 00:30:45,520
+اللي هو ال enzymes تبعه بيستغلوا ال host cell DNA 
+Polymerase
+399
+00:30:45,520 --> 00:30:48,320
+parvo viruses 
+are the exception because they replicate in the
+
+400
+00:30:48,320 --> 00:30:52,660
+cytoplasm where they do not have access to the
+
+401
+00:30:52,660 --> 00:30:56,120
+host cell RNA polymerase بجه هذا مضطر يجيب الانزيم
+
+402
+00:30:56,120 --> 00:31:00,600
+تبعه معاه اللي هو ال parvo virus وبالتالي ما يمكنش نجي
+
+403
+00:31:00,600 --> 00:31:04,360
+لل RNA virus ع السريع هدا كلهم double stranded
+
+404
+00:31:04,360 --> 00:31:07,000
+هدولة single stranded وطبيعته أصلا أن يكون single
+
+405
+00:31:07,000 --> 00:31:11,220
+strand ال RNA لكن في exceptions أكثر من اللي هو ال
+
+406
+00:31:12,650 --> 00:31:16,230
+Except for members of the reovirus family which
+
+407
+00:31:16,230 --> 00:31:22,950
+have a double-stranded RNA من
+
+408
+00:31:22,950 --> 00:31:30,930
+الريو فايروسز
+
+409
+00:31:30,930 --> 00:31:35,790
+في نموذج مشهور جدا اسمه rotavirus
+
+410
+00:31:41,020 --> 00:31:44,220
+الثالثة مرتبط بالفيكال أورال ترانسميشن فيكال أورال
+
+411
+00:31:44,220 --> 00:31:52,180
+ترانسميشن فيكال
+
+412
+00:31:52,180 --> 00:31:55,360
+أورال ترانسميشن فيكال أورال ترانسميشن فيكال أورال
+
+413
+00:31:55,360 --> 00:31:56,380
+ترانسميشن فيكال أورال ترانسميشن فيكال أورال
+
+414
+00:31:56,380 --> 00:31:58,020
+ترانسميشن فيكال أورال ترانسميشن فيكال أورال
+
+415
+00:31:58,020 --> 00:31:58,800
+ترانسميشن فيكال أورال ترانسميشن فيكال أورال
+
+416
+00:31:58,800 --> 00:32:00,720
+ترانسميشن فيكال أورال ترانسميشن فيكال أورال
+
+417
+00:32:00,720 --> 00:32:04,700
+ترانسميشن فيكال أورال ترانسميشن فيكال أورال
+
+418
+00:32:04,700 --> 00:32:09,960
+ترانسميشن فيكال الـ hepatitis D defective هو الثالث
+
+419
+00:32:09,960 --> 00:32:11,560
+اللي احنا حاولنا أن نحكي عنه يبقى فيه اثنين رئيسين
+
+420
+00:32:11,560 --> 00:32:15,940
+وواحد ثالث هدولة إيش مالهم؟ اللي هم خطوات تكاثر
+
+421
+00:32:15,940 --> 00:32:20,720
+في وين؟ في النواة .. في النواة .. أنا أتكلم عن ال
+
+422
+00:32:20,720 --> 00:32:29,670
+RNA virus مثلا عندنا ال retro virusretrovirus
+
+423
+00:32:29,670 --> 00:32:36,910
+integrate a DNA copy بيدمج قطعة من ال .. أو نسخة
+
+424
+00:32:36,910 --> 00:32:40,690
+من ال DNA تبعه في ال host's chromosome أو ال cell
+
+425
+00:32:40,690 --> 00:32:43,970
+دي بيجوانوا بحكومهم على الكلام أن هو أكيد في أن هو 
+
+426
+00:32:43,970 --> 00:32:49,370
+influenza virus synthesizes their progeny نسلهم
+
+427
+00:32:49,370 --> 00:32:53,390
+genome in the nucleus والآخر بيقولنا اللي هو
+
+428
+00:32:56,270 --> 00:32:59,770
+بتقولنا كمان إيه الخطوات في اللي هو النواة إذا
+
+429
+00:32:59,770 --> 00:33:02,630
+لو حد على فكرة فيها كثير معلومات فيها بتقولنا أن
+
+430
+00:33:02,630 --> 00:33:09,550
+كلهم double-stranded ما عدا ال parvovirus كلهم
+
+431
+00:33:09,550 --> 00:33:12,950
+في النواة ما عدا ال parvo virus الثانيين ال RNA virus
+
+432
+00:33:12,950 --> 00:33:17,350
+كلهم single-stranded ما عدا الreovirus كلهم في
+
+433
+00:33:17,350 --> 00:33:20,870
+الcytoplasm ما عدا ثلاثة اللي هم ال retrovirus وال
+
+434
+00:33:20,870 --> 00:33:26,190
+influenza و زاد اللي هو ال hepatitis delta مهمة جدا
+
+435
+00:33:26,190 --> 00:33:30,410
+اللي هو العملية طيب تلعبوا على المنظر هذا بس و
+
+436
+00:33:30,410 --> 00:33:35,690
+اعرفوا الأنواع double stranded viruses
+
+437
+00:33:35,690 --> 00:33:40,770
+وأنا راح على طول على النواة inborn
+
+438
+00:33:40,770 --> 00:33:43,510
+and able بدل وعصر تبلازم صاروا transcription early
+
+439
+00:33:43,510 --> 00:33:47,470
+البروتينات اللي بتصنع ترجع على النواة بتساعد في
+
+440
+00:33:47,470 --> 00:33:51,330
+اللي هو تصنيع المادة الوراثية وكمان replication و
+
+441
+00:33:51,330 --> 00:33:56,070
+transcription مرة أخرى لل late proteins وأنا صار
+
+442
+00:33:56,070 --> 00:34:00,470
+زحمة في الخلية الصحية صار شغل كثير يعني فال early
+
+443
+00:34:00,470 --> 00:34:03,730
+.. ال early .. أنا زي ما بقول .. بقول مثل نموذج
+
+444
+00:34:03,730 --> 00:34:08,290
+لما الواحد بيجيب يكتب ورقة أو كتاب بيجيب كتب برضه
+
+445
+00:34:08,290 --> 00:34:12,770
+فالكتاب جيبت عشان أعمل منه كتاب أو أعمل منه كتب
+
+446
+00:34:12,770 --> 00:34:17,470
+تمام؟ فال .. يعني ال early مهم لتصنيع اللي هو ال
+
+447
+00:34:17,470 --> 00:34:21,450
+late protein synthesis نستكمل إن شاء الله للموضوع
+
+448
+00:34:23,030 --> 00:34:28,370
+الآن الفيروسات قلنا ال RNA هو ال ultimate goal
+
+449
+00:34:28,370 --> 00:34:32,330
+وبالتالي هناك استراتيجية مختلفة للفيروسات للوصول
+
+450
+00:34:32,330 --> 00:34:37,050
+لل mRNA فمثلا أبسط وسيلة .. إذا أنا أقول لك مباشرة
+
+451
+00:34:37,050 --> 00:34:43,830
+اعطيني الجلم، مصبوط؟ إذا بداخله منك طلع ال virus من 
+
+452
+00:34:43,830 --> 00:34:47,450
+استراتيجيته البسيطة أن هو أصلا positive stranded
+
+453
+00:34:47,450 --> 00:34:51,750
+تمام وبالتالي it acts as a messenger mRNA ما لوش
+
+454
+00:34:51,750 --> 00:34:57,670
+أي معالجة مسبقة في هذا الموضوع بمعنى it acts as a
+
+455
+00:34:57,670 --> 00:35:01,050
+template مباشرة لـ translation النوع الثاني اللي هو
+
+456
+00:35:01,050 --> 00:35:04,550
+second group بقولنا has a single strand RNA of
+
+457
+00:35:04,550 --> 00:35:07,110
+negative polarity طب negative polarity ماهي هي ..
+
+458
+00:35:07,110 --> 00:35:13,370
+هي مُشرّتنا أن ما فيش عندنا mRNA عفوا .. ريبوزوم يقرا
+
+459
+00:35:13,370 --> 00:35:16,690
+mRNA مباشرة لأن بتاعي لازم نعمل complementation
+
+460
+00:35:16,690 --> 00:35:19,830
+إيش هي complementation؟ ال negative أنصنع منه ..
+
+461
+00:35:19,830 --> 00:35:24,240
+بص في .. وبعدين خدوا بالكوا في هذه المرحلة ال
+
+462
+00:35:24,240 --> 00:35:28,920
+positive can act يعني لغرضين الغرض الأول أن بالفعل
+
+463
+00:35:28,920 --> 00:35:33,020
+mRNA وكمان ك template كقالب يصنع منه ال genome ما 
+
+464
+00:35:33,020 --> 00:35:35,300
+هو ال genome negative stranded صح؟ فبيصير
+
+465
+00:35:35,300 --> 00:35:39,820
+complementary له أن يصنع منه negative strand army
+
+466
+00:35:39,820 --> 00:35:43,880
+أمثلة عليها اللي هو ال measles virus وال rabies
+
+467
+00:35:43,880 --> 00:35:48,960
+virus وفيها اللي هو الهدوء اللي بيمتلكه بقطعة
+
+468
+00:35:48,960 --> 00:35:53,660
+واحدة على فكرة من ال RNA ال negative بينها في نوع
+
+469
+00:35:53,660 --> 00:35:56,940
+ثاني بيحتوي على multiple pieces اللي هم ال
+
+470
+00:35:56,940 --> 00:36:01,260
+influenza virus خدوا بالكوا أي فيروس بيحتوي على ال
+
+471
+00:36:01,260 --> 00:36:04,580
+negative sense مضطر يجيب معاه إنزيمات تبعده بتاعه
+
+472
+00:36:04,580 --> 00:36:09,580
+و non-infectious كجينوم كجينوم non-infectious the
+
+473
+00:36:09,580 --> 00:36:14,690
+third group has double-stranded RNA Double-stranded
+
+474
+00:36:14,690 --> 00:36:20,410
+يعني في عنده negative وفي عنده positive لكن لأن
+
+475
+00:36:20,410 --> 00:36:23,410
+خلايانا لا تحتوي على أي إنزيم تتعامل مع double
+
+476
+00:36:23,410 --> 00:36:27,450
+-stranded فهو يجيب برضه عشان يفصلها ويصنع
+
+477
+00:36:27,450 --> 00:36:43,370
+negative و positive الريو فايروس الريو
+
+478
+00:36:43,370 --> 00:36:43,550
+فايروس الريو فايروس الريو فايروس الريو فايروس
+
+479
+00:36:43,550 --> 00:36:44,850
+الريو فايروس الريو فايروس الريو فايروس الريو
+
+480
+00:36:44,850 --> 00:36:46,830
+فايروس الريو فايروس الريو فايروس الريو فايروس
+
+481
+00:36:46,830 --> 00:36:47,350
+الريو فايروس الريو فايروس الريو فايروس الريو
+
+482
+00:36:47,350 --> 00:36:54,930
+فايروس الريو فايروس الريو فايروس الريو فايروس
+
+483
+00:36:54,930 --> 00:37:02,120
+الريو فايروس الريو فايروس الشكل الرابع من ال RNA
+
+484
+00:37:02,120 --> 00:37:06,500
+فيروس هم المفروض أنهم ثلاثة، يا إما positive، يا إما
+
+485
+00:37:06,500 --> 00:37:09,980
+negative، يا إما double-stranded في الأسف الرابع هو
+
+486
+00:37:09,980 --> 00:37:13,520
+positive strand لكنه حالة خاصة، positive strand حالة
+
+487
+00:37:13,520 --> 00:37:19,080
+خاصة هو HIV، لحظة محتوى عليها قطعة وقطعة، هي
+
+488
+00:37:19,080 --> 00:37:24,520
+قطعتين، هدول القطعتين مش قطعتين مختلفتين، هم
+
+489
+00:37:24,520 --> 00:37:28,840
+identical بغير هذا القطع التابعون الفيروس اللي
+
+490
+00:37:28,840 --> 00:37:31,880
+جابهم بشوية أو الانفلونزا الثمان قطع هدول كل قطعة
+
+491
+00:37:31,880 --> 00:37:36,650
+تختلف عن هذا identical اللي أنا سميناهم deployed
+
+492
+00:37:36,650 --> 00:37:41,010
+فاكرين؟ بقولنا أن كل الفيروسات hub load ماعدا
+
+493
+00:37:41,010 --> 00:37:47,110
+retro viruses are deployed طبعا هذه كلمة retro بقى
+
+494
+00:37:47,110 --> 00:37:50,370
+نهائية الخلف زمان إحنا كنا نعرف أن ال flow of
+
+495
+00:37:50,370 --> 00:37:54,730
+information هو من ال DNA لل RNA لل protein بيسموها
+
+496
+00:37:54,730 --> 00:37:59,010
+ال central dogma لما اكتشفنا الفيروسات صار عندنا
+
+497
+00:37:59,010 --> 00:38:03,880
+يعني لأ في أشياء جديدة ساندبرج على ورا ال RNA
+
+498
+00:38:03,880 --> 00:38:07,400
+بالضبط هو من ال RNA ل .. وهذا مش عندنا عند
+
+499
+00:38:07,400 --> 00:38:10,380
+الفيروس اختار هذا الانزيم تبعه ويجب أن يكون معاه
+
+500
+00:38:10,380 --> 00:38:16,380
+اللي هو reverse transcriptase وهذه الفكرة تبع ال 
+
+501
+00:38:16,380 --> 00:38:19,160
+infectious nucleic acid أنا شرحتها أنه إذا كان
+
+502
+00:38:19,160 --> 00:38:24,820
+قادر على تخطي الـ specificity  ويعني أنه عمل
+
+503
+00:38:24,820 --> 00:38:28,220
+infection بعد الحكي أنه infectious nucleic acid
+
+504
+00:38:28,220 --> 00:38:29,160
+إذا ما جدرش
+
+505
+00:38:35,050 --> 00:38:40,030
+الشكل اللي قدامنا هذا يمكن اللي .. اللي هي المقادة
+
+506
+00:38:40,030 --> 00:38:43,410
+اللي بدأنا فيها لما قلنا أنه كل الفيروسات
+
+507
+00:38:43,410 --> 00:38:46,130
+بأنواعها المختلفة double-stranded single-stranded
+
+508
+00:38:46,130 --> 00:38:49,030
+DNA أو RNA segmented أو غير segmented بتروح على
+
+509
+00:38:49,030 --> 00:38:55,470
+مين على أنها تتحول لـ viral mRNA وإن شاء الله بتوضح لكم 
+
+510
+00:38:55,470 --> 00:38:57,650
+الموضوع هذا لاحقًا بإذن الله
+
+511
+00:39:02,870 --> 00:39:07,350
+once the viral mRNA of either DNA or RNA virus is
+
+512
+00:39:07,350 --> 00:39:11,510
+synthesized it is translated by the host ribosome
+
+513
+00:39:11,510 --> 00:39:14,430
+into the viral protein يبقى ياخذوا بالكم الـ
+
+514
+00:39:14,430 --> 00:39:18,830
+ribosome بتاعتنا الصحيح very fair neutral بتقول
+
+515
+00:39:18,830 --> 00:39:23,930
+للخليجي أنا ما ليش دخل أنا هاتولي أي كتاب أنا بقراه
+
+516
+00:39:23,930 --> 00:39:29,290
+ما بُميز فيروس كتاب فيروس كتاب أنا بعرف أن هذا الـ
+
+517
+00:39:29,290 --> 00:39:36,840
+mRNA محتاج القياله بقراهمشاكلكوا جابلي إذا وصلتني
+
+518
+00:39:36,840 --> 00:39:42,920
+إذا وصلتني أنا بقرأ ما له داعي نيوترال في الموجة
+
+519
+00:39:42,920 --> 00:39:47,980
+بتتكون mRNA بوستر بولاريتي بيجي لريبوزوم و بيقرأ
+
+520
+00:39:47,980 --> 00:39:53,380
+طريقة القراءة الآن هو المشكلة
+
+521
+00:39:53,380 --> 00:39:59,720
+بيقول إنه some of which are early proteins
+
+522
+00:40:08,840 --> 00:40:15,380
+هذا الجدول ب يوضح لنا القائمة بالفيروسات و الـ
+
+523
+00:40:15,380 --> 00:40:21,480
+polymerase المسئولة عن تصنيع الـ RNA من المادة
+
+524
+00:40:21,480 --> 00:40:21,960
+الوراثية
+
+525
+00:40:26,540 --> 00:40:31,920
+محتاجة أو معتمدة على الـ cell polymerases وفي DNA
+
+526
+00:40:31,920 --> 00:40:36,320
+viruses معتمدة على أنزيمتها نفسها فاكرين لما قلنا
+
+527
+00:40:36,320 --> 00:40:42,380
+أن الـ box virus ما يقدرش يروح على الـ nucleus
+
+528
+00:40:42,380 --> 00:40:45,800
+وبالتالي هو محتاج أن الانزيم الخاص فيه اللي مش
+
+529
+00:40:45,800 --> 00:40:49,280
+هقدر يطوله من وين من النوات بتاعته الـ host فهكذا
+
+530
+00:40:49,280 --> 00:40:55,310
+ونفس الجثة بالنسبة للـ RNA virus فكرة الـ precursor
+
+531
+00:40:55,310 --> 00:40:58,070
+polypeptide اللي شرحتها قبل قليل أنه الـ
+
+532
+00:40:58,070 --> 00:41:05,510
+translation تم لـ full length mRNA ويطلع عندي
+
+533
+00:41:05,510 --> 00:41:11,370
+polypeptide طويل يتم تقطيعه بعد ذلك من أشهر
+
+534
+00:41:11,370 --> 00:41:16,770
+النماذج إن حدثنا بيكورنا فيروس و الـ picorna pico
+
+535
+00:41:16,770 --> 00:41:20,530
+معناه small و RNA بيكو عملي
+
+536
+00:41:32,060 --> 00:41:37,460
+الجينوم تبعها روية RNA acting as mRNA is
+
+537
+00:41:37,460 --> 00:41:41,220
+translated into a single polypeptide which is then
+
+538
+00:41:41,220 --> 00:41:43,780
+cleaved by a virus coded protease into various
+
+539
+00:41:43,780 --> 00:41:45,860
+proteins الكلام اللي حكيناه في بداية المحاضرة،
+
+540
+00:41:45,860 --> 00:41:48,720
+قلنا إن الـ protease بيقطعها إلى functional
+
+541
+00:41:53,560 --> 00:41:57,960
+في البداية ففكرنا أنه unique للمجموعة دي اللي
+
+542
+00:41:57,960 --> 00:42:01,700
+جالنا في الآخر لأنه هو استراتيجية معتمدة لدى كثير
+
+543
+00:42:01,700 --> 00:42:06,860
+منها من البيانات التبتالي بدأنا نبحث عن فكرة أنه
+
+544
+00:42:06,860 --> 00:42:10,060
+نستهدف من الـ proteins اللي بيجدع هذا الـ
+
+545
+00:42:10,060 --> 00:42:14,800
+polyurethane بتالي احنا بنضمن أنه نخلص منه فهي
+
+546
+00:42:14,800 --> 00:42:18,400
+مثلًا الـ retro virus و الـ retro virus أنا هحكيلكم
+
+547
+00:42:18,400 --> 00:42:24,980
+بكل وضوح أن اكتشاف الـ HIV عمل advancement كبيرة في
+
+548
+00:42:24,980 --> 00:42:28,380
+علم الفيروسات وعلم المناعة في علم الفيروسات وعلم
+
+549
+00:42:28,380 --> 00:42:32,920
+المناعة الـ full اكتر فيروس دُرست الـ life cycle
+
+550
+00:42:32,920 --> 00:42:36,440
+تبقى تبقى دقة هو الـ HIV في محاولة من العلماء اللي
+
+551
+00:42:36,440 --> 00:42:42,070
+هتطبق إنهم يجدوا علاج cute له من خلال دراستنا
+
+552
+00:42:42,070 --> 00:42:47,110
+للفيروس ولما ندرسها الـ life cycle ندرسها مش عشان
+
+553
+00:42:47,110 --> 00:42:50,550
+احنا بدنا نعرفها و خلاص، معرفتها لا علم إلا ما
+
+554
+00:42:50,550 --> 00:42:55,430
+نفع، لا علم إلا ما نفع، بالمعرفة بدنا نعرف وين
+
+555
+00:42:55,430 --> 00:43:00,480
+نستهدفها عشان نوقف اللي هو الـ life cycle فلما
+
+556
+00:43:00,480 --> 00:43:04,000
+عرفنا إنه قعد أشهر الجينات تبعتها اللي هو الجاج
+
+557
+00:43:04,000 --> 00:43:06,580
+والـ pol genes اللي بيصير لهم translation برضه
+
+558
+00:43:06,580 --> 00:43:11,680
+بيكونوا بول بيبطال لازم يجسهم من الـ protease إذا
+
+559
+00:43:11,680 --> 00:43:16,280
+أنا استغليت الـ protease أنا وقفت أهم two genes
+
+560
+00:43:16,280 --> 00:43:20,620
+وبالتالي وقفت عملية الـ violence و replication تبع
+
+561
+00:43:20,620 --> 00:43:23,460
+اللي هو الـ violence وبالفعل الآن في class
+
+562
+00:43:27,140 --> 00:43:31,540
+فهو يستخدم في علاج الـ Retrovirus اللي هي الـ HIV
+
+563
+00:43:31,540 --> 00:43:36,480
+منها في أننا كمان مثال تاني الـ Flaviviruses الـ
+
+564
+00:43:36,480 --> 00:43:40,960
+Flaviviruses مثال شهير عليها اللي هو الـ Hepatitis
+
+565
+00:43:40,960 --> 00:43:44,620
+C والـ Yellow Fever فيروس برضه بس بيعملوا
+
+566
+00:43:44,620 --> 00:43:47,380
+precursor polypeptide في أننا كمان الـ Rota
+
+567
+00:43:50,300 --> 00:43:54,760
+النموذج التالي اللي هو الـ Influenza و الـ Rotavirus
+
+568
+00:43:54,760 --> 00:43:59,700
+عندهم segmented genome وبالتالي كل قطعة بيصيلها
+
+569
+00:43:59,700 --> 00:44:02,920
+transcription أو translation بتعطينا functional
+
+570
+00:44:02,920 --> 00:44:05,380
+إيه الـ protein لأن في precursor protein وفي
+
+571
+00:44:05,380 --> 00:44:11,180
+functional protein مباشرة هذه لوحة نموذج على الـ
+
+572
+00:44:11,180 --> 00:44:14,500
+polyvirus اللي بيوضح لنا أنه long polypeptide long
+
+573
+00:44:14,500 --> 00:44:20,000
+mRNA بتكون منه long أو full length وهذا طبعًا الـ
+
+574
+00:44:20,000 --> 00:44:27,620
+cleavage عبارة عن تحويل من inactive إلى active، من
+
+575
+00:44:27,620 --> 00:44:30,120
+non-functional إلى functional، ونفس الشيء اللي
+
+576
+00:44:30,120 --> 00:44:32,940
+بتفكره عندي هنا كمان للـ retrovirus
+
+577
+00:44:38,810 --> 00:44:43,910
+طبعًا الـ virusات اللي عندها proteases و اللي همية
+
+578
+00:44:43,910 --> 00:44:46,470
+طبية فيه نماذج عليها في الـ dead well هذا بـ
+
+579
+00:44:46,470 --> 00:44:50,250
+coronavirus, bloody virus, toga virus, coronavirus
+
+580
+00:44:50,250 --> 00:44:54,470
+و الـ retroviruses طبعًا كل واحد منهم بيوضحلنا في الـ
+
+581
+00:44:54,470 --> 00:44:58,610
+dead well إيش هو الـ polypeptide أو الـ polyprotein
+
+582
+00:44:58,610 --> 00:45:02,890
+و وين بيتم عملية اللي هو site of cleavage تخيلوا
+
+583
+00:45:02,890 --> 00:45:05,230
+الـ retroviruses الـ cleavage وين صار؟
+
+584
+00:45:08,130 --> 00:45:12,950
+في الـ bonding layer يعني و الجريب ما تطلع الفيروس
+
+585
+00:45:12,950 --> 00:45:18,330
+بيصير فيه عملية cleavage لذلك إذا استهدفنا بنمنع
+
+586
+00:45:18,330 --> 00:45:20,670
+الـ bonding تبعه إذا منعنا الـ bonding تبعه ما يروح
+
+587
+00:45:20,670 --> 00:45:24,750
+إنه بطل يطلع إلى الخلايا الأخرى ولن يكون بعمل
+
+588
+00:45:24,750 --> 00:45:29,710
+infection لـ other cells الفكرة
+
+589
+00:45:29,710 --> 00:45:33,790
+هذه ما كنتش أحكي فيها كثير لكن هي الفكرة إنه دائمًا
+
+590
+00:45:33,790 --> 00:45:39,650
+أبدأ في المادة الوطنية الـ T بدو يجيبلها A والـ G
+
+591
+00:45:39,650 --> 00:45:43,490
+بدو يجيبلها C أو الـ C يجيبلها وها كده فإذا حاطوا
+
+592
+00:45:43,490 --> 00:45:48,530
+انتوا في كل الفيروسات اللي شفناها إذا المادة اللي
+
+593
+00:45:48,530 --> 00:45:54,170
+بيبقى بيها double stranded بدها تنقسم تنقسم إلى
+
+594
+00:45:54,170 --> 00:45:58,770
+two strands تبعها ويتكملوا complementary صح؟ خلصنا
+
+595
+00:45:58,770 --> 00:46:02,470
+منهم إذا كان single strand بدو يتكمل إذا كان
+
+596
+00:46:02,470 --> 00:46:04,670
+negative بدو positive وإذا كان positive بدو
+
+597
+00:46:04,670 --> 00:46:09,910
+negative وها كده فكل الفكرة معتمدة على الـ
+
+598
+00:46:09,910 --> 00:46:13,910
+complementarity اللي هي التكامل أنه فيروس أو مادة
+
+599
+00:46:13,910 --> 00:46:19,350
+وضعيفية كامل ومادة وضعيفية آخر وإذا ربحوا في الدول
+
+600
+00:46:19,350 --> 00:46:23,370
+الغالية كثير الـ parental genome و الـ progeny
+
+601
+00:46:23,370 --> 00:46:27,430
+genome إذا حد بيقدر يجيلي اختلاف بكون شاطر
+
+602
+00:46:31,670 --> 00:46:37,210
+مش اختلاف، يبقى مش كلكم شطرين، لأنه أصلا الهدف أنه
+
+603
+00:46:37,210 --> 00:46:41,930
+أنا أطلّي النسخ برجيني نفس الـ .. الـ ventrella، صح
+
+604
+00:46:41,930 --> 00:46:45,570
+دلوقتي؟ طب أنا أجي postive sense، single strand
+
+605
+00:46:45,570 --> 00:46:48,950
+عرنية، ونفس الشيء اللي برجيني هيكون، لكن الـ
+
+606
+00:46:48,950 --> 00:46:52,870
+intermediate عكسه، الـ intermediate اللي هو داخل الـ
+
+607
+00:46:52,870 --> 00:46:55,510
+.. و أنا بعمل replication، مش قولنا postive sense
+
+608
+00:46:55,510 --> 00:46:57,850
+اللي بتكون له إيه؟ negative sense، هاي الـ
+
+609
+00:46:57,850 --> 00:46:59,390
+complementarity اللي احنا بنحكيه
+
+610
+00:47:02,710 --> 00:47:06,030
+Assembly لأن بني ليزا بلقت فيها أنت بتذكرها و
+
+611
+00:47:06,030 --> 00:47:09,790
+بتلكوا أن الـ assembly معتمد بالأساس على الـ
+
+612
+00:47:09,790 --> 00:47:13,250
+interaction اللي احنا مش فاهمينه ما بين الـ protein
+
+613
+00:47:13,250 --> 00:47:18,150
+و المادة الوراثية وهذا اعتقادنا أنه ناتج عن الـ
+
+614
+00:47:18,150 --> 00:47:20,490
+chemical structure أو الـ chemical composition
+
+615
+00:47:20,490 --> 00:47:26,410
+للمادة الثانية المادة DNA أو الـ RNA وهذا الكلام
+
+616
+00:47:26,410 --> 00:47:27,930
+للأسف يعني ..
+
+617
+00:47:30,950 --> 00:47:33,330
+النقطة اللي حكيتها لكم يعني أنه لما حبطناهم مع
+
+618
+00:47:33,330 --> 00:47:41,050
+بعض اتجمعوا عملوا virion كامل اللقطة
+
+619
+00:47:41,050 --> 00:47:43,010
+اللي أمامكم هي اللقطة اللي شفناها في الـ
+
+620
+00:47:43,010 --> 00:47:50,090
+animation أنه فيروس هو طالع من الخلية بياخد جزء من
+
+621
+00:47:50,090 --> 00:47:54,910
+الـ cell membrane بعد ما يكون قد يعني وضع فيه اللي
+
+622
+00:47:54,910 --> 00:47:56,470
+هو الـ glycoproteins
+
+623
+00:48:01,480 --> 00:48:05,680
+receptor للـ next infection مهانة ميزة تقولنا الـ
+
+624
+00:48:05,680 --> 00:48:08,680
+envelope viruses إنها بتسهل عليها عملية اللي هو
+
+625
+00:48:08,680 --> 00:48:12,140
+الارتباط أو الإصابة هذا الموضوع مش هاجده أكمله
+
+626
+00:48:12,140 --> 00:48:16,240
+اليوم يعني في ضيق الوقت فهو إيه يعني ممكن تلت أربع
+
+627
+00:48:16,240 --> 00:48:20,080
+شرائع أرجو أن تقراهم لحالك واللي عنده أي استفسار 
+
+628
+00:48:20,080 --> 00:48:24,980
+إن شاء الله أنا باستقبله بس بدي أختم بيعني بكلمات 
+
+629
+00:48:24,980 --> 00:48:25,640
+بسيطة
+
+630
+00:48:31,750 --> 00:48:35,310
+الطلبة يعني تبين أنه ما بنجرّش نحكم عليها من خلال
+
+631
+00:48:35,310 --> 00:48:37,910
+الامتحان يعني في ناس عامة ال presentation والصحي
+
+632
+00:48:37,910 --> 00:48:41,550
+كانت ال presentation تبعها جيدة جداً هذا بيقودنا
+
+633
+00:48:41,550 --> 00:48:44,310
+إلى أنه يعني أنا شخصياً أنا عندي قناعة من زمان أنه 
+
+634
+00:48:44,310 --> 00:48:48,450
+موضوع تقييم الطلبة من خلال الامتحان هذا يعني وسيلة 
+
+635
+00:48:48,450 --> 00:48:52,530
+من الوسائل ولكن ليس هو التقييم الحقيقي واللي حتى
+
+636
+00:48:52,530 --> 00:48:56,490
+بيرسب عندي بضل الاحترام والتقدير تبعه موجود يعني
+
+637
+00:48:56,490 --> 00:49:01,380
+بمعنى آخر مش لأنه هو رسب أو بما يكون ظرف الآخر لكن 
+
+638
+00:49:01,380 --> 00:49:05,200
+احنا بنقول لكم أن هذا المساق ليس للنسيان ليس للامتحان
+
+639
+00:49:05,200 --> 00:49:09,440
+وبعدين نطلعّه من أدمغتنا ونمشي هذا مساق احنا 
+
+640
+00:49:09,440 --> 00:49:13,360
+محتاجينه طول الطريق وبعد الطريق وبعد اللي هو
+
+641
+00:49:13,360 --> 00:49:17,620
+التخرج وبالتالي أنا بأأمن منكم أنكم تدرسوه بعناية
+
+642
+00:49:17,620 --> 00:49:22,340
+أكثر من أنه ندرس عشان بدنا نجيب علامات في الختام
+
+643
+00:49:22,340 --> 00:49:26,440
+ندعو الله أن يوفقكم إلى ما فيه الخير والسلام عليكم
+
+644
+00:49:26,440 --> 00:49:27,080
+ورحمة الله وبركاته
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/VIGilQ1fnrY_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/VIGilQ1fnrY_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..70d8b7ff910ac0d1e999aa62c71a273f96fe1fc2
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/VIGilQ1fnrY_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 4994, "start": 20.94, "end": 49.94, "text": "بسم الله الرحمن الرحيم الحمد لله رب العالمين والصلاة والسلام وعلى سيدنا محمد وعلى آله وصحبه ومن ساعة دربه اليوم الدين هذا يوم ختم محاضرتنا ان شاء الله نعمل من الله انه يكون كان فصل دراسي مثمر واستفدنا منه معلومات تؤهلنا إلى المرحلة التانية اللي هي مرحلة diagnostic microbiology اليوم هنكمل بجزئية اسمها viral replication انا ابتديت انه نختم فيها لانه", "tokens": [3555, 38251, 21984, 34892, 5016, 27842, 34892, 5016, 32640, 21542, 2304, 3215, 24976, 3224, 12602, 3555, 18863, 45340, 9957, 16070, 9381, 15040, 3660, 16070, 3794, 37440, 4032, 3615, 23942, 8608, 25708, 8315, 3714, 35571, 3215, 4032, 3615, 23942, 19753, 43761, 4032, 9381, 5016, 3555, 3224, 4032, 27842, 8608, 995, 27884, 11778, 25513, 3224, 45595, 20498, 32748, 9957, 23758, 7251, 20498, 16490, 39237, 3714, 5016, 46958, 43500, 8315, 16472, 13412, 16606, 21984, 8717, 25957, 1211, 9154, 21984, 16472, 3224, 7251, 30544, 25961, 6156, 36520, 11778, 23557, 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الحالة functional على طرف ال polypeptide بيكون قطعة بيسموها protease انزين protease تفصل حالها من حالها من القطع هذه و بتبدأ تجتف ال polypeptide من أماكن محددة طبعا كل قطعة تنتج بيسميها functional functional polypeptide", "tokens": [9381, 5016, 5296, 11296, 2445, 20328, 5016, 5296, 11296, 8978, 29538, 21542, 6027, 3660, 11745, 15844, 23032, 28480, 2423, 6754, 494, 662, 482, 4724, 1829, 30544, 12174, 9566, 27884, 4724, 1829, 38251, 2407, 11296, 5631, 651, 16472, 11622, 9957, 5631, 651, 6055, 5172, 36520, 11331, 6027, 11296, 9154, 11331, 6027, 11296, 9154, 25062, 9566, 3615, 29538, 4032, 39894, 44510, 10721, 6055, 7435, 2655, 5172, 2423, 6754, 494, 662, 482, 9154, 5551, 15042, 19452, 3714, 24401, 41891, 23032, 3555, 3615, 995, 28242, 12174, 9566, 27884, 6055, 29399, 7435, 4724, 1829, 38251, 1829, 11296, 11745, 11745, 6754, 494, 662, 482], "avg_logprob": -0.19633837872081333, "compression_ratio": 1.8316831683168318, "no_speech_prob": 0.0, "words": [{"start": 974.04, "end": 974.32, "word": 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1628.84, "end": 1647.38, "text": "بتستغربوا احنا كنا نقول انه يعني مثلا في أشياء زي الأجار بتكون صلبة بنسخنها تسعة وتمانية وتسعين بتكون لسه صلبة تسعة وتسعين بتصير سائلة، مظبوط؟ درجة حرارة واحدة بتغير من طبيعات المركب", "tokens": [3555, 2655, 14851, 17082, 25513, 14407, 1975, 5016, 8315, 9122, 8315, 8717, 39648, 16472, 3224, 37495, 22653, 50113, 15040, 8978, 5551, 8592, 1829, 16606, 30767, 1829, 16247, 7435, 9640, 39894, 30544, 20328, 46152, 3660, 44945, 3794, 9778, 1863, 11296, 6055, 3794, 27884, 34683, 2304, 7649, 10632, 34683, 3794, 3615, 9957, 39894, 30544, 5296, 3794, 3224, 20328, 46152, 3660, 6055, 3794, 27884, 34683, 3794, 3615, 9957, 39894, 9381, 13546, 8608, 16373, 37977, 12399, 3714, 19913, 3555, 2407, 9566, 22807, 11778, 47341, 3660, 11331, 2288, 9640, 3660, 36764, 24401, 3660, 39894, 17082, 13546, 9154, 23032, 21292, 3615, 9307, 9673, 31747, 3555], "avg_logprob": -0.12632812991738318, "compression_ratio": 1.7619047619047619, "no_speech_prob": 8.344650268554688e-07, "words": 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" وبالفعل", "probability": 0.82275390625}, {"start": 2602.66, "end": 2602.88, "word": " الآن", "probability": 0.666259765625}, {"start": 2602.88, "end": 2603.0, "word": " في", "probability": 0.92578125}, {"start": 2603.0, "end": 2603.46, "word": " class", "probability": 0.9580078125}], "temperature": 1.0}, {"id": 104, "seek": 262738, "start": 2607.14, "end": 2627.38, "text": "فهو يستخدم في علاج الـ Retrovirus اللي هي الـ HIV منها فى أننا كمان مثال تانى الـ Flaviviruses الـ Flaviviruses مثال شهير عليها اللي هو الـ Hepatitis C والـ Yellow Fever فيروس برضه بس بيعملوا precursor polypeptide فى أننا كمان الـ Rota", "tokens": [5172, 3224, 2407, 7251, 14851, 9778, 40448, 8978, 11203, 26108, 2423, 39184, 11495, 24088, 9619, 13672, 1829, 39896, 2423, 39184, 15907, 9154, 11296, 6156, 7578, 14739, 8315, 9122, 2304, 7649, 50113, 6027, 6055, 7649, 7578, 2423, 39184, 3235, 706, 592, 347, 8355, 2423, 39184, 3235, 706, 592, 347, 8355, 50113, 6027, 13412, 3224, 13546, 25894, 11296, 13672, 1829, 31439, 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2657.56, "text": "النموذج التالي اللي هو ال Influenza و ال Rotavirus عندهم segmented genome وبالتالي كل قطعة بيصيلها transcription أو translation بتعطينا functional إيه ال protein لأن في precursor protein و فينا functional protein مباشرة هذه لوحة نموذج على ال polyvirus اللي بيوضح لنا انه long polypeptide long mRNA بتكون منه long أو full length", "tokens": [6027, 1863, 2304, 2407, 8848, 7435, 16712, 6027, 1829, 13672, 1829, 31439, 2423, 11537, 2781, 23691, 4032, 2423, 17681, 10868, 43242, 16095, 9469, 292, 21953, 46599, 6027, 2655, 6027, 1829, 28242, 12174, 9566, 27884, 4724, 1829, 9381, 1829, 1211, 11296, 35288, 34051, 12853, 39894, 3615, 9566, 1829, 8315, 11745, 11933, 1829, 3224, 2423, 7944, 5296, 33456, 8978, 41736, 284, 7944, 4032, 8978, 8315, 11745, 7944, 3714, 3555, 33599, 25720, 29538, 45164, 5016, 3660, 8717, 2304, 2407, 8848, 7435, 15844, 2423, 6754, 85, 9619, 13672, 1829, 4724, 1829, 2407, 11242, 5016, 5296, 8315, 16472, 3224, 938, 6754, 494, 662, 482, 938, 50103, 39894, 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"word": " long", "probability": 0.72216796875}, {"start": 2654.5, "end": 2655.02, "word": " mRNA", "probability": 0.98291015625}, {"start": 2655.02, "end": 2655.82, "word": " بتكون", "probability": 0.817138671875}, {"start": 2655.82, "end": 2656.12, "word": " منه", "probability": 0.964111328125}, {"start": 2656.12, "end": 2656.6, "word": " long", "probability": 0.9326171875}, {"start": 2656.6, "end": 2657.06, "word": " أو", "probability": 0.60205078125}, {"start": 2657.06, "end": 2657.3, "word": " full", "probability": 0.66796875}, {"start": 2657.3, "end": 2657.56, "word": " length", "probability": 0.61669921875}], "temperature": 1.0}, {"id": 106, "seek": 267294, "start": 2658.86, "end": 2672.94, "text": "وهذا طبعا ال cleavage عبارة عن تحويل من inactive إلى active، من non-functional إلى functional، ونفس الشيء اللي بتفكره عندي هنا كمان لل retrovirus", "tokens": [2407, 3224, 15730, 23032, 3555, 3615, 995, 2423, 1233, 706, 609, 6225, 3555, 9640, 3660, 18871, 6055, 5016, 2407, 26895, 9154, 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هو", "probability": 0.97900390625}, {"start": 2695.77, "end": 2696.33, "word": " ال", "probability": 0.84033203125}, {"start": 2696.33, "end": 2697.69, "word": " polypeptide", "probability": 0.8990478515625}, {"start": 2697.69, "end": 2697.83, "word": " أو", "probability": 0.7958984375}, {"start": 2697.83, "end": 2697.93, "word": " ال", "probability": 0.91943359375}, {"start": 2697.93, "end": 2698.61, "word": " polyprotein", "probability": 0.891845703125}, {"start": 2698.61, "end": 2699.21, "word": " و", "probability": 0.9365234375}, {"start": 2699.21, "end": 2699.69, "word": " وين", "probability": 0.85986328125}, {"start": 2699.69, "end": 2700.05, "word": " بيتم", "probability": 0.6416829427083334}, {"start": 2700.05, "end": 2700.41, "word": " عملية", "probability": 0.9256184895833334}, {"start": 2700.41, "end": 2700.55, "word": " اللي", "probability": 0.9521484375}, {"start": 2700.55, "end": 2700.75, "word": " هو", "probability": 0.96435546875}, {"start": 2700.75, "end": 2701.33, 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"word": " محتاجينه", "probability": 0.9728515625}, {"start": 2950.2, "end": 2950.92, "word": " طول", "probability": 0.996337890625}, {"start": 2950.92, "end": 2951.54, "word": " الطريق", "probability": 0.9918619791666666}, {"start": 2951.54, "end": 2952.12, "word": " وبعد", "probability": 0.6131998697916666}, {"start": 2952.12, "end": 2952.68, "word": " الطريق", "probability": 0.9908854166666666}, {"start": 2952.68, "end": 2953.14, "word": " وبعد", "probability": 0.6009521484375}, {"start": 2953.14, "end": 2953.26, "word": " اللي", "probability": 0.959716796875}, {"start": 2953.26, "end": 2953.36, "word": " هو", "probability": 0.9853515625}, {"start": 2953.36, "end": 2953.92, "word": " التخرج", "probability": 0.9661458333333334}, {"start": 2953.92, "end": 2954.72, "word": " وبالتالي", "probability": 0.858984375}, {"start": 2954.72, "end": 2954.86, "word": " انا", "probability": 0.727294921875}, {"start": 2954.86, "end": 2955.34, "word": " بأمن", "probability": 0.87255859375}, {"start": 2955.34, "end": 2955.62, "word": " منكم", "probability": 0.897216796875}, {"start": 2955.62, "end": 2955.88, "word": " انكوا", "probability": 0.709716796875}, {"start": 2955.88, "end": 2956.84, "word": " تدرسوه", "probability": 0.9695638020833334}, {"start": 2956.84, "end": 2957.62, "word": " بعناية", "probability": 0.8582356770833334}, {"start": 2957.62, "end": 2958.56, "word": " اكثر", "probability": 0.687744140625}, {"start": 2958.56, "end": 2958.78, "word": " من", "probability": 0.98486328125}, {"start": 2958.78, "end": 2959.16, "word": " انه", "probability": 0.873046875}, {"start": 2959.16, "end": 2959.44, "word": " ندرس", "probability": 0.9932861328125}, {"start": 2959.44, "end": 2959.64, "word": " عشان", "probability": 0.9734700520833334}, {"start": 2959.64, "end": 2960.06, "word": " بدنا", "probability": 0.769287109375}, {"start": 2960.06, "end": 2960.66, "word": " نجيب", "probability": 0.995361328125}, {"start": 2960.66, "end": 2961.08, "word": " علامات", "probability": 0.9811197916666666}, {"start": 2961.08, "end": 2961.84, "word": " في", "probability": 0.6494140625}, {"start": 2961.84, "end": 2962.34, "word": " الختام", "probability": 0.8889973958333334}, {"start": 2962.34, "end": 2963.0, "word": " ندعو", "probability": 0.9189453125}, {"start": 2963.0, "end": 2963.18, "word": " الله", "probability": 0.9560546875}, {"start": 2963.18, "end": 2963.4, "word": " ان", "probability": 0.76904296875}, {"start": 2963.4, "end": 2964.2, "word": " يوفقكم", "probability": 0.9825439453125}, {"start": 2964.2, "end": 2964.54, "word": " إلى", "probability": 0.76025390625}, {"start": 2964.54, "end": 2964.74, "word": " ما", "probability": 0.822265625}, {"start": 2964.74, "end": 2965.18, "word": " فيه", "probability": 0.79443359375}, {"start": 2965.18, "end": 2965.8, "word": " الخير", "probability": 0.98486328125}, {"start": 2965.8, "end": 2966.16, "word": " والسلام", "probability": 0.87744140625}, {"start": 2966.16, "end": 2966.44, "word": " عليكم", "probability": 0.9150390625}, {"start": 2966.44, "end": 2966.62, "word": " ورحمة", "probability": 0.89013671875}, {"start": 2966.62, "end": 2966.74, "word": " الله", "probability": 0.94580078125}, {"start": 2966.74, "end": 2967.08, "word": " وبركاته", "probability": 0.95126953125}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 2968.67125, "duration_after_vad": 2726.0756249999895}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/Wu7cQmVXlBc.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/Wu7cQmVXlBc.srt
new file mode 100644
index 0000000000000000000000000000000000000000..b9d0f5b5f1bf7ca588717235644d26d4c7c1943c
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/Wu7cQmVXlBc.srt
@@ -0,0 +1,2579 @@
+1
+00:00:20,920 --> 00:00:23,120
+السلام عليكم ورحمة الله وبركاته بسم الله الرحمن
+
+2
+00:00:23,120 --> 00:00:27,120
+الرحيم أنواع المحاضرة اليوم هو  sterilization 
+
+3
+00:00:27,120 --> 00:00:31,760
+and disinfection وهذا الموضوع يأتي بعد سلسلة
+
+4
+00:00:31,760 --> 00:00:36,560
+مواضيع كلها كانت مركزة على تركيب الخلية ومن ثم
+
+5
+00:00:36,560 --> 00:00:40,820
+كيفية تنمية الخلية وما هي العوامل اللي تؤثر على
+
+6
+00:00:40,820 --> 00:00:45,420
+النمو بدل منمي البكتيريا الحقيقة أنا ذكرت في نهاية
+
+7
+00:00:45,420 --> 00:00:49,560
+المحاضرة السابقة أنّه ليس من الصعب تنمية البكتيريا
+
+8
+00:00:49,560 --> 00:00:54,020
+وإنما اللي صعب أو أصعب هو كيف أنا أتخلص من
+
+9
+00:00:54,020 --> 00:01:00,580
+البكتيريا واليوم هنبدأ الموضوع هذا ببعض المصطلحات
+
+10
+00:01:00,580 --> 00:01:05,460
+ومن ثم بعض الأدوات وبعض التقنيات المستخدمة في
+
+11
+00:01:05,460 --> 00:01:07,960
+السيطرة على الـ micro organism أو الـ control of
+
+12
+00:01:07,960 --> 00:01:13,950
+microorganism بنعرف أنّه من خلال التعريف اللي مورده
+
+13
+00:01:13,950 --> 00:01:17,350
+لنا sterilization أنّه مصطلح مطلق كلمة مصطلح مطلق
+
+14
+00:01:17,350 --> 00:01:22,210
+يعني لا يقبل الاستثناء بمعنى أنّه بقولنا هنا is the
+
+15
+00:01:22,210 --> 00:01:26,810
+killing or removal of all microorganisms كلمة all
+
+16
+00:01:26,810 --> 00:01:31,170
+تعني المتبقاش zero وبالتالي هو بالفعل absolute
+
+17
+00:01:31,170 --> 00:01:35,510
+term بدنا دائماً نميزه يعني ما نخلط فيهم الأيام و
+
+18
+00:01:35,510 --> 00:01:40,310
+نقول sterilization و disinfection و disinfection
+
+19
+00:01:40,310 --> 00:01:44,550
+هم نفس المصطلح لأ باختلفوا عن بعض اختلاف كبير الـ
+
+20
+00:01:44,550 --> 00:01:49,330
+sterilization absolute term بينما disinfection لأ
+
+21
+00:01:49,330 --> 00:01:50,730
+بيقبل الـ compromise 
+
+22
+00:01:54,260 --> 00:01:56,960
+إذا بتلاحظوا الـ sterilization is usually carried
+
+23
+00:01:56,960 --> 00:02:01,980
+out using autoclaving الـ autoclaving أو الـ
+
+24
+00:02:01,980 --> 00:02:05,400
+autoclave هو أداة من الأدوات اللي كلكم بتعرفوها في
+
+25
+00:02:05,400 --> 00:02:09,720
+المنزل اللي هي عبارة عن pressure cooker أو تنجرة
+
+26
+00:02:09,720 --> 00:02:15,240
+الضغط احنا بنعرف أنّه نقطة غليان الماء هي 100 درجة
+
+27
+00:02:15,240 --> 00:02:19,360
+مئة أوي مئة درجة مئة أوي لو سخننا نص ساعة ساعة
+
+28
+00:02:19,360 --> 00:02:25,290
+ساعتين تلاتة في الآخر بتضلها مئة مئة درجة مئوية إذا
+
+29
+00:02:25,290 --> 00:02:30,670
+كيف نحصل على درجة أعلى من تلك الدرجة؟ عن طريق ضغط
+
+30
+00:02:30,670 --> 00:02:37,810
+بخار الماء نضغط البخار فترتفع الحرارة بمقدار الضغط
+
+31
+00:02:37,810 --> 00:02:42,970
+يعني بمثال مولود اليوم على الـ سطولة لو كان الضغط 15
+
+32
+00:02:42,970 --> 00:02:47,650
+PSI 15 PSI هو المصطلح الأكثر شيوعاً في الاستخدام
+
+33
+00:02:47,650 --> 00:02:52,440
+وهو عبارة عن pound per square inch square inch اللي
+
+34
+00:02:52,440 --> 00:02:55,980
+هي بوصة مربعة والـ bound هو راطلينجيز يعني هي من
+
+35
+00:02:55,980 --> 00:03:01,180
+قياسة إذا كانت ضغط 15 psi بتكون درجة حرارة الماء
+
+36
+00:03:01,180 --> 00:03:08,440
+121 درجة مئوية لو احتفظنا بهذه الدرجة و هذا الضغط
+
+37
+00:03:08,440 --> 00:03:17,940
+لمدة 15 دقيقة تمام هنا نحصل على تعقيم بمعنى أن كل
+
+38
+00:03:17,940 --> 00:03:24,330
+المخلوقات الطبية اللي بنعرفها تموت عند هذه الدرجة
+
+39
+00:03:24,330 --> 00:03:29,910
+لكن بدنا نكون متأكدين أنّه بالفعل الدرجة اللي
+
+40
+00:03:29,910 --> 00:03:32,110
+قدامنا اللي على الـ .. اللي .. اللي على الـ counter
+
+41
+00:03:32,110 --> 00:03:36,790
+أنّها 121 وإن الضغط صحيح وإن الـ timing بدأ .. متى
+
+42
+00:03:36,790 --> 00:03:41,570
+بدأ الـ timing؟ بعد ما وصلت الحرارة لـ 121 درجة
+
+43
+00:03:41,570 --> 00:03:45,790
+مئوية يعني مش من أول العملية لأ بعد وصول العملية
+
+44
+00:03:45,790 --> 00:03:50,940
+إلى 121 درجة مئوية ثلاثة أرقام سهلة مئة واحد وعشرين
+
+45
+00:03:50,940 --> 00:03:52,820
+خمسة عشر خمسة عشر خمسة عشر خمسة عشر خمسة عشر خمسة عشر
+
+46
+00:03:52,820 --> 00:03:55,560
+خمسة عشر خمسة عشر خمسة عشر خمسة عشر خمسة عشر خمسة عشر
+
+47
+00:03:55,560 --> 00:03:58,980
+خمسة عشر
+
+48
+00:03:58,980 --> 00:04:02,060
+خمسة عشر
+
+49
+00:04:02,060 --> 00:04:04,100
+خمسة عشر خمسة عشر خمسة عشر خمسة عشر خمسة عشر خمسة عشر
+
+50
+00:04:04,100 --> 00:04:04,400
+خمسة عشر خمسة عشر خمسة عشر خمسة عشر خمسة عشر خمسة عشر
+
+51
+00:04:04,400 --> 00:04:04,740
+خمسة عشر خمسة عشر خمسة عشر خمسة عشر خمسة عشر خمسة عشر
+
+52
+00:04:04,740 --> 00:04:05,620
+خمسة عشر خمسة عشر خمسة عشر خمسة عشر خمسة عشر خمسة عشر
+
+53
+00:04:05,620 --> 00:04:17,900
+خمسة عشر خمسة عشر خمسة عشر خمسة عشر خمسة عشر
+
+54
+00:04:17,900 --> 00:04:23,410
+خمسة عشر الشاشة الطبية على سبيل المثال لكن هناك أدوات قد
+
+55
+00:04:23,410 --> 00:04:29,370
+تتدمر بالتعقيم بمعنى بروتين لو بنعقم الـ albumin
+
+56
+00:04:29,370 --> 00:04:32,770
+تبع البيض وحطيناها في الـ autoclave درجة الحرارة
+
+57
+00:04:32,770 --> 00:04:36,930
+هتكون إيه؟ عالية جداً بحيث أن الـ albumin هيصير له
+
+58
+00:04:36,930 --> 00:04:41,090
+coagulation بيبطل السائل بيصير صلب طب في هذه
+
+59
+00:04:41,090 --> 00:04:44,010
+الحالة أنا بدي البروتين هذا بدي الـ albumin
+
+60
+00:04:44,010 --> 00:04:47,170
+بديها سائل ليش هي صلب؟ يبقى ماقدرش أعقمه بالـ
+
+61
+00:04:47,170 --> 00:04:50,410
+autoclave يبقى الـ autoclave على الرغم من شيوعه
+
+62
+00:04:50,410 --> 00:04:55,430
+وانتشاره وكثرة استخداماته أنّه غير صالح لكل
+
+63
+00:04:55,430 --> 00:05:00,250
+الأغراض اللي احنا من أجلها بنعقم الأشياء فبقولنا
+
+64
+00:05:00,250 --> 00:05:04,050
+مثلاً surgical instruments that may rust رست يعني
+
+65
+00:05:04,050 --> 00:05:09,430
+بتصدأ في وجود اللي هو البخار الماء، لا يستطيعون
+
+66
+00:05:09,430 --> 00:05:13,630
+يعقينه الـ autoclave جهاز لابتوب زي هذا لو صار عليه
+
+67
+00:05:13,630 --> 00:05:17,330
+spill، bacterial spill وانكبت عليه مزرعة بكتيريا،
+
+68
+00:05:17,330 --> 00:05:21,810
+وبدأ نعقمه بنفع نحطه في الـ autoclave؟ طيب بنفع نضيل
+
+69
+00:05:21,810 --> 00:05:26,390
+عليه كلور؟ ما نفعش يبقى بأحسن وسيلة تعقيمه في هذه
+
+70
+00:05:26,390 --> 00:05:31,350
+الحالة للغاز أنّه نجيب مادة غازية قاتلة الغاز
+
+71
+00:05:31,350 --> 00:05:36,850
+بيخترق الأشياء دون أن يحدث بلل أو wet وبالتالي
+
+72
+00:05:36,850 --> 00:05:41,070
+بتظل الدوائر الكهربائية شغالة إذن فين دي أشكال
+
+73
+00:05:41,070 --> 00:05:44,730
+وأنواع متختلفة من اللي هو المواد المستخدمة له الـ
+
+74
+00:05:44,730 --> 00:05:48,530
+sterilization ألا بالله حاجة ذكرت واحد تعريف رقم
+
+75
+00:05:48,530 --> 00:05:53,550
+اثنين ذكرت الطريقة اللي بتتم من خلالها the math
+
+76
+00:05:53,550 --> 00:05:57,750
+اللي زي اللي هو الـ autoclaving ثم تحدثت على بدائل
+
+77
+00:05:57,750 --> 00:06:02,890
+أخرى تحدثت عن مثلاً الـ ethane oxide اللي هو
+
+78
+00:06:02,890 --> 00:06:06,930
+الغاز في هذه الحالة وفي كمان حالة تالتة موجودة على
+
+79
+00:06:06,930 --> 00:06:10,610
+اللوحة اللي هي الـ filtration أنا أقول كده بشري الـ
+
+80
+00:06:10,610 --> 00:06:14,070
+الـ albumin صح؟ الـ albumin إذا أنا بديها protein
+
+81
+00:06:14,070 --> 00:06:17,870
+السائل كما هو بمرره عبر filter الـ filter هذا
+
+82
+00:06:17,870 --> 00:06:21,310
+مساماته اللي هتسبح بمرور البكتيريا والفيروسات
+
+83
+00:06:21,310 --> 00:06:24,990
+وبالتالي كل ما يمر أو لما السائل يمر عبره
+
+84
+00:06:24,990 --> 00:06:30,050
+البكتيريا والفيروسات تحجز إلى الأعلى وبنزل
+
+85
+00:06:30,050 --> 00:06:33,910
+نستقبل السائل في أنبوبة معقمة وبالتالي سيجعلنا
+
+86
+00:06:33,910 --> 00:06:38,380
+تعقيم بالـ filtration إذن هناك وسائل مختلفة بس
+
+87
+00:06:38,380 --> 00:06:40,960
+بيتلاحظوا شغل من اللوحة هذه قبل ما ننتقل للوحة
+
+88
+00:06:40,960 --> 00:06:44,920
+تانية أن هناك من الوسائل اللي ذكرناها وسائل
+
+89
+00:06:44,920 --> 00:06:49,000
+ميكانيكية زي الـ filtration وسائل فيزيائية زي
+
+90
+00:06:49,000 --> 00:06:54,240
+الحرارة وسائل كيميائية زي اللي هو الغاز إذا غالباً
+
+91
+00:06:54,240 --> 00:06:58,220
+هنقسم الـ methods إلى physical and chemical methods
+
+92
+00:06:58,220 --> 00:07:02,240
+لكن لسه ما خلصنا من التعريفات وبالتالي بدنا نكمل
+
+93
+00:07:02,240 --> 00:07:06,870
+موضوع التعريفات أنا من البداية قاعد بحذر من الخلط
+
+94
+00:07:06,870 --> 00:07:11,350
+ما بين المصطلحين اللي هو sterilization اللي هو الـ
+
+95
+00:07:11,350 --> 00:07:12,490
+sterilization اللي هو الـ sterilization اللي هو الـ
+
+96
+00:07:12,490 --> 00:07:13,710
+sterilization اللي هو الـ sterilization اللي هو الـ
+
+97
+00:07:13,710 --> 00:07:14,690
+sterilization اللي هو الـ sterilization اللي هو الـ
+
+98
+00:07:14,690 --> 00:07:15,710
+sterilization اللي هو الـ sterilization اللي هو الـ
+
+99
+00:07:15,710 --> 00:07:16,130
+sterilization اللي هو الـ sterilization اللي هو الـ
+
+100
+00:07:16,130 --> 00:07:16,550
+sterilization اللي هو الـ sterilization اللي هو الـ
+
+101
+00:07:16,550 --> 00:07:16,950
+sterilization اللي هو الـ sterilization اللي هو الـ
+
+102
+00:07:16,950 --> 00:07:17,450
+sterilization اللي هو الـ sterilization اللي هو الـ
+
+103
+00:07:17,450 --> 00:07:17,990
+sterilization اللي هو الـ sterilization اللي هو الـ
+
+104
+00:07:17,990 --> 00:07:18,150
+sterilization اللي هو الـ sterilization اللي هو الـ
+
+105
+00:07:18,150 --> 00:07:18,770
+sterilization اللي هو الـ sterilization اللي هو الـ
+
+106
+00:07:18,770 --> 00:07:22,290
+sterilization اللي هو الـ sterilization اللي هو الـ
+
+107
+00:07:22,290 --> 00:07:25,210
+sterilization اللي هو الـ sterilization اللي هو الـ
+
+108
+00:07:25,210 --> 00:07:29,310
+sterilization اللي هو الـ sterilization اللي 
+
+109
+00:07:36,910 --> 00:07:40,610
+الآن في مصطلح أو مصطلحين بدنا ننتبه لهم جيداً
+
+110
+00:07:40,610 --> 00:07:47,190
+disinfectants ومصطلح ثاني اسمه antiseptic أنتَ
+
+111
+00:07:47,190 --> 00:07:53,570
+هتكلم عن مين؟ disinfection الـ antiseptic أو الـ
+
+112
+00:07:53,570 --> 00:08:00,390
+disinfectant بيحمل نفس التعريف مادة تستخدم لمين؟
+
+113
+00:08:02,000 --> 00:08:06,960
+لكن لماذا ميزنا بينهم بالاسم الـ disinfectants
+
+114
+00:08:06,960 --> 00:08:11,360
+تستخدم للـ inanimate objects يعني الأسطح الغير حية
+
+115
+00:08:11,360 --> 00:08:16,120
+بينما الـ antiseptic بنستخدمه للأسطح الحية ولنفترض
+
+116
+00:08:16,120 --> 00:08:20,600
+الجل يعني الكحول اللي بنستخدمه اللي هو الـ 70%
+
+117
+00:08:20,600 --> 00:08:25,200
+alcohol اللي بنستخدمه لـ skin disinfection هذا
+
+118
+00:08:25,200 --> 00:08:29,630
+عبارة عن عياش antiseptic لو استخدمته لمسح الطاولة
+
+119
+00:08:29,630 --> 00:08:32,830
+أو تطهير الطاولة بيكون اسمها يبقى هو اللي أنا
+
+120
+00:08:32,830 --> 00:08:37,870
+استخدمناها لغرضين مختلفين فأخد اسمين مختلفين أخد
+
+121
+00:08:37,870 --> 00:08:40,510
+مرة Antiseptic لما استخدمته على الجلد وأخد اسم
+
+122
+00:08:40,510 --> 00:08:46,290
+ثاني اعتقده من الصورة وأضحى في هذا المجال طيب ما
+
+123
+00:08:46,290 --> 00:08:49,970
+الذي يجعلنا نقرر استخدام مادة على الجلد ومادة
+
+124
+00:08:49,970 --> 00:08:54,070
+أخرى على الأسطح عشان تقول أنت ما تأثرش على اللي
+
+125
+00:08:54,070 --> 00:08:58,290
+أنا بدأت .. يعني بدأ أطهره يعني لو .. لو افترضنا
+
+126
+00:08:58,290 --> 00:09:00,950
+إنه ماء النار .. ماء النار اللي هي الـhydrochloric
+
+127
+00:09:00,950 --> 00:09:05,130
+acid قاتلة للبكتيريا، أتميزاش نحطها على الجلد؟
+
+128
+00:09:05,130 --> 00:09:07,070
+احنا طبعًا .. احنا ما نستخدمهاش، لكن بنستخدم 
+
+129
+00:09:07,070 --> 00:09:10,710
+الـchlorine على سمك متر، الـchlorine معروف عنه قاتل
+
+130
+00:09:10,710 --> 00:09:15,870
+جيد للبكتيريا، لكن بيعمل skin irritation، يعني 
+
+131
+00:09:15,870 --> 00:09:19,350
+تهيج في الجلد، وقد يعمل skin burn، كمان حرق في 
+
+132
+00:09:19,350 --> 00:09:23,170
+الجلد، طب ما نستخدموش على الجلد، بنستخدموه على .. على
+
+133
+00:09:23,170 --> 00:09:27,800
+أسطح، الـiodine أقل حدًّا من مين من الكلورين، مع أنه
+
+134
+00:09:27,800 --> 00:09:31,340
+نوع من نفس الفصيلة الكلورين .. أقل الهالوجينس،
+
+135
+00:09:31,340 --> 00:09:36,580
+لكن بنستخدمه على مين؟ على الجروح، ما نستخدموش على
+
+136
+00:09:36,580 --> 00:09:42,680
+الأسطح لأنه بيترك stain، أثر، مظبوط؟ فهو mild على
+
+137
+00:09:42,680 --> 00:09:45,980
+الـskin و سيء لمين؟ للأسطح، إذًا اللي بيقول لنا 
+
+138
+00:09:45,980 --> 00:09:49,580
+نقرر إنه نستخدمه لسه infected أو unseptic هو الـ
+
+139
+00:09:49,580 --> 00:09:52,660
+property بنستبعده، هل هو toxic، هل بيعمل stain،
+
+140
+00:09:52,660 --> 00:09:56,550
+أعطيكم مثال ثالث، بتعرفوا الـcrystal violet الـ
+
+141
+00:09:56,550 --> 00:10:00,610
+crystal violet اللي هو اسم ثاني جنتيشان فيولت هذا
+
+142
+00:10:00,610 --> 00:10:04,370
+معروف عنه المركب أنه عنده قدرة على قتل البكتيريا
+
+143
+00:10:04,370 --> 00:10:08,610
+واستخدمناه في وسط اسمه الماكونكي، بتعرفوا الماكونكي؟
+
+144
+00:10:08,610 --> 00:10:14,230
+الماكونكي selective media أضفنا عليه crystal
+
+145
+00:10:14,230 --> 00:10:18,250
+violet حتى نمنع الـgram-positive بكتيريا، يبقى هو
+
+146
+00:10:18,250 --> 00:10:22,630
+القاتل لمين؟ للـgram-positive، طيب ليش ما نستخدمه احنا
+
+147
+00:10:22,630 --> 00:10:26,450
+في الـdisinfection و الـantiseptic؟ و للأسف 
+
+148
+00:10:26,450 --> 00:10:30,270
+ما نستخدموش للغرضين لأنه بيترك stain، لو أنا 
+
+149
+00:10:30,270 --> 00:10:33,950
+استخدمته على إيدي بدي أسبوع أو أسبوعين حتى اللون
+
+150
+00:10:33,950 --> 00:10:38,970
+يروح بعد غسيل مستمر، بيجيب و يترك أثر، وبالتالي لم
+
+151
+00:10:38,970 --> 00:10:42,670
+نستخدمه لا على الأسطح ولا على .. استخدمناه ليه؟
+
+152
+00:10:42,670 --> 00:10:45,530
+أغراض معينة زي مثلًا كنا في الأوساط الزراعية
+
+153
+00:10:49,180 --> 00:10:51,320
+هذه اللغة مهمة كثير كثير كثير كثير كثير كثير كثير
+
+154
+00:10:51,320 --> 00:10:51,680
+كثير كثير كثير كثير كثير كثير كثير كثير كثير كثير
+
+155
+00:10:51,680 --> 00:10:56,700
+كثير كثير كثير كثير كثير كثير كثير كثير كثير كثير
+
+156
+00:10:56,700 --> 00:11:04,340
+كثير كثير كثير
+
+157
+00:11:04,340 --> 00:11:05,640
+كثير كثير كثير كثير كثير كثير كثير كثير كثير كثير
+
+158
+00:11:05,640 --> 00:11:07,980
+كثير كثير كثير كثير كثير كثير كثير كثير كثير كثير
+
+159
+00:11:07,980 --> 00:11:17,000
+كثير كثير كثير كثير كثير
+
+160
+00:11:17,000 --> 00:11:25,230
+كثير، درجات لغاية ما الآن بدنا نطلع على نقطتين
+
+161
+00:11:25,230 --> 00:11:28,430
+مهمتين أو مهمّين في عملية الـkilling للـmicro
+
+162
+00:11:28,430 --> 00:11:34,910
+organism، أنه لما أنا بدي أفحص قدرة agent على قتل
+
+163
+00:11:34,910 --> 00:11:38,650
+البكتيريا، بنعد البكتيريا اللي ماتت، بعد البكتيريا
+
+164
+00:11:38,650 --> 00:11:42,810
+اللي بقيت حية، هذا اللي بقدر أعمله أنا، أنا بقدر أعدّ
+
+165
+00:11:42,810 --> 00:11:46,570
+البكتيريا الحية، أما اللي ماتت ما بقدرش أعدّها، فبكون
+
+166
+00:11:46,570 --> 00:11:51,630
+عارف العدد الأصلي، ولما البكتيريا الحيّة قعدّها،
+
+167
+00:11:51,630 --> 00:11:55,250
+بتروح و بيضلّ منها في الميت، فإحنا غالبًا بنتعامل مع
+
+168
+00:11:55,250 --> 00:12:00,690
+البكتيريا التي بقيت حيّة، الـsurvivors، من
+
+169
+00:12:00,690 --> 00:12:05,590
+البكتيريا، بنقول إن الـnumber of survivors ده 
+
+170
+00:12:05,590 --> 00:12:10,810
+البكتيريا تتناسب تناسبًا عكسيًا مع C و T، إيش الـC و
+
+171
+00:12:10,810 --> 00:12:14,990
+T؟ الـC هو الـconcentration of the killing agent،
+
+172
+00:12:14,990 --> 00:12:19,810
+أعتقد أنه بقول لكم قرأته على اللوحة أو على البطل تبع
+
+173
+00:12:19,810 --> 00:12:24,930
+الكحول اللي في المختبر 70% الكحول، وكلمة 70% هي
+
+174
+00:12:24,930 --> 00:12:29,930
+concentration، ليش ما بيقولنا 50%، 80%، مظبوط؟ يبقى في
+
+175
+00:12:29,930 --> 00:12:35,230
+الآخر 70% معناها concentration، the length of time
+
+176
+00:12:35,230 --> 00:12:40,610
+agent is applied، أنتم بتعرفوا أنه الزمن عامل مهم
+
+177
+00:12:40,610 --> 00:12:45,310
+في كل حاجة، وأعطيكم مثال بسيط، لو ولعنا ولع أو
+
+178
+00:12:45,310 --> 00:12:50,690
+بلعنا ولع الغاز، حطينا إيدينا على مسافة مثلًا عشرين
+
+179
+00:12:50,690 --> 00:12:58,430
+سم، هل بنشعر بلسعة النار فورًا ولا بعد فترة معينة
+
+180
+00:12:58,430 --> 00:13:02,170
+ما نقدرش نتحمل الحرارة، فالتأثير إيش كان عبارة عن
+
+181
+00:13:02,170 --> 00:13:06,810
+تراكمي مع الزمن، تراكمي مع الزمن، يبقى الـexposure
+
+182
+00:13:06,810 --> 00:13:12,010
+time أو الـlength of time agent is applied is
+
+183
+00:13:12,010 --> 00:13:16,090
+very essential، مهم جدًا، يبقى فيه two factors، إيش هم
+
+184
+00:13:16,090 --> 00:13:20,590
+كم مرة؟ الـconcentration و الـtime، بنقول إن الـ
+
+185
+00:13:20,590 --> 00:13:23,710
+number of survivors تلاتة، و تلاتة سببين، عكسيًا مع
+
+186
+00:13:23,710 --> 00:13:27,630
+الـconcentration و الـtime، يعني كلما زاد الـtime
+
+187
+00:13:27,630 --> 00:13:31,630
+كلما زاد الـconcentration، كلما قل عدد .. اسم .. مش كل
+
+188
+00:13:31,630 --> 00:13:36,940
+.. يعني كلما تكون العلاقة عكسية، عكسيًا، ما بيحكي إنه
+
+189
+00:13:36,940 --> 00:13:40,560
+كل ما زاد الزمن، كل ما زاد الـconcentration، كل ما
+
+190
+00:13:40,560 --> 00:13:42,920
+قل على الـsurvival، يعني بقى مناعة، كل ما زاد
+
+191
+00:13:42,920 --> 00:13:46,720
+القتل، كل ما زاد القتل، الجالسة النقطة كتير معلومة
+
+192
+00:13:46,720 --> 00:13:51,780
+أو مفهومة، ويجب أن نستفيد منها، بنفعنا نيجي نمسح
+
+193
+00:13:51,780 --> 00:13:55,400
+الأسطح اللي في المستشفيات عشان بس نقول والله
+
+194
+00:13:55,400 --> 00:14:00,100
+طهرنا، لازم نترك وقت، زمن، معتمد على الـ
+
+195
+00:14:00,100 --> 00:14:02,640
+concentration، يعني لو كانت الـconcentration
+
+196
+00:14:02,640 --> 00:14:06,420
+خمسين، حنحسب له زمن أطول من لو كانت الـconcentration
+
+197
+00:14:06,420 --> 00:14:10,000
+سبعين، هدول العاملين متفاعلين مع بعض، الـ
+
+198
+00:14:10,000 --> 00:14:13,480
+concentration هي والزمن، طب خدوا بالكم، احنا بعض
+
+199
+00:14:13,480 --> 00:14:17,460
+الأحيان هدول العاملين مع بعض، يعني بيجبرونا أن
+
+200
+00:14:17,460 --> 00:14:22,740
+نفكر بطرق متنوعة، الكحول على سبيل المثال، تركيز
+
+201
+00:14:22,740 --> 00:14:26,390
+خمسين بالمئة بيقتل البكتيريا، لكن الزمن اللازم بده 
+
+202
+00:14:26,390 --> 00:14:30,070
+تلات دقائق تقريبًا، تلات دقائق، في حدّ فينا بيغسل إيديه
+
+203
+00:14:30,070 --> 00:14:34,270
+بالكحول، بيقعد فيهم تلات دقائق، كم دقيقة، كم ثانية،
+
+204
+00:14:34,270 --> 00:14:37,430
+ثواني بنستخدم صح ولا ثواني، وانتهى الموضوع، ف
+
+205
+00:14:37,430 --> 00:14:40,450
+practically احنا بنرفع الـconcentration عشان نقلل
+
+206
+00:14:40,450 --> 00:14:44,210
+من الزمن في هذه الحالة، لكن خدوا بالكم، و لكل
+
+207
+00:14:44,210 --> 00:14:48,750
+قاعدة دايمًا فيها شوية، الكحول بنسبة مائة بالمائة
+
+208
+00:14:54,050 --> 00:14:59,230
+الكحول بنسبة مائة بالمائة غير فعال، بس بنسبة تسعين
+
+209
+00:14:59,230 --> 00:15:03,190
+بالمائة فعال، وتمانين بالمائة فعال، وسبعين، وستين،
+
+210
+00:15:03,190 --> 00:15:08,250
+وخمسين، ليش اخترنا سبعين بالذات؟ لأننا وجدنا إن الـ70 at
+
+211
+00:15:08,250 --> 00:15:13,070
+one second أو two seconds كافية للقتل، طب وأنا بقدر
+
+212
+00:15:13,070 --> 00:15:16,170
+أمسح إيدي بأقل من one second؟ أصلاً ما بقدرش، وبالتالي
+
+213
+00:15:16,170 --> 00:15:19,730
+مناسبة، طب مش مختلف بنستخدم إيش تمانين؟ تمانين أغلى
+
+214
+00:15:19,730 --> 00:15:24,790
+تمانين بتكلفني فلوس، concentration أعلى، مظبوط
+
+215
+00:15:24,790 --> 00:15:28,710
+بتكلفني مادة فعالة أعلى، وبالتالي فيها فلوس أكثر
+
+216
+00:15:28,710 --> 00:15:31,970
+يبقى في أكثر من عامل بيخلينا نختار إيش الـrise
+
+217
+00:15:31,970 --> 00:15:35,920
+concentration، لو روحنا على المستشفى وقلنا لهم يا
+
+218
+00:15:35,920 --> 00:15:39,060
+جماعة، إيش في عندكم مواد مطهرة بتستخدموها في
+
+219
+00:15:39,060 --> 00:15:44,800
+المستشفى؟ هيذكرونا مجموعة أسماء، حنسألهم ألا يوجد
+
+220
+00:15:44,800 --> 00:15:50,260
+لديكم نوع واحد بيغني عن كل هدول الأشياء؟ هيقولون لنا
+
+221
+00:15:50,260 --> 00:15:55,280
+للأسف لأ، للأسف لأ، ذكرنا أحد الأسباب أنه ممكن يكون
+
+222
+00:15:55,280 --> 00:15:59,760
+.. أعطيكم مثال أنا من حياتي كل يومية، بدك تشتري
+
+223
+00:15:59,760 --> 00:16:05,010
+laptop، حتقول لي بدي مواصفات، بدي كارت شاشة مواصفاته
+
+224
+00:16:05,010 --> 00:16:08,510
+مش عارف اتنين جيجا، بدي hard disk واحد تيرا، بدي
+
+225
+00:16:08,510 --> 00:16:12,630
+الـmemory تبعته مش عارف الـcash تبعه وكده، إنهم
+
+226
+00:16:12,630 --> 00:16:15,790
+بروحي السوق بنلاقي تلاتة من الأربع مواصفات اللي
+
+227
+00:16:15,790 --> 00:16:20,230
+طلبناهم، قولين في laptop، والتاني اتنين من أربع،
+
+228
+00:16:20,230 --> 00:16:26,990
+واحد من أربع كمان، لكن للأسف ما نلاقيش laptop واحد
+
+229
+00:16:26,990 --> 00:16:31,170
+اللي فيه الأحلام تبعتنا، اللي بدنا إياها احنا في
+
+230
+00:16:31,170 --> 00:16:35,320
+المواصفات، فبنضطر إنه إذا أنا بحتاج هذه المواصفات
+
+231
+00:16:35,320 --> 00:16:37,680
+إن أنا أشتري لابتوب هذا، وأشتري لابتوب هذا، وأشتري
+
+232
+00:16:37,680 --> 00:16:41,580
+لابتوب هذا، إذا بستخدمه لأغراض متنوعة ومختلفة
+
+233
+00:16:41,580 --> 00:16:45,280
+وهذا اللي هو بيصير بالفعل في المختبرات، الـideal
+
+234
+00:16:45,280 --> 00:16:50,200
+antiseptic أو disinfectant مو موجود، يعني it must
+
+235
+00:16:50,200 --> 00:16:54,080
+be cheap، لازم يكون رخيص، it must be effective at
+
+236
+00:16:54,080 --> 00:16:58,960
+low concentration، it must be safe، ما فيش irritation
+
+237
+00:16:58,960 --> 00:17:02,430
+مالوش رائحة كريهه، على الأقل بروح على المستشفى
+
+238
+00:17:02,430 --> 00:17:06,070
+بلاقيهم ناطفين الأرضيات بكلور، ريحة المستشفى تكون
+
+239
+00:17:06,070 --> 00:17:09,490
+سيئة للغاية، صح؟ دلوقت وقد تؤذي المريض، بعض الناس
+
+240
+00:17:09,490 --> 00:17:13,910
+حتتحسسوا من الـ .. في مواد قاتلة جدًا للبكتيريا
+
+241
+00:17:13,910 --> 00:17:18,750
+وسامة للإنسان، فبالتالي المواصفات المثالية كمان
+
+242
+00:17:18,750 --> 00:17:22,750
+مرة اللي احنا بنحكيها مش متوفرة في agent واحد، لكن
+
+243
+00:17:22,750 --> 00:17:26,170
+ممكن نلاقي في هذا الـagent خمسة من عشرة، ستة من
+
+244
+00:17:26,170 --> 00:17:29,230
+عشرة، سبعة من عشرة، وبالتالي بنستخدمه لأغراض محددة
+
+245
+00:17:35,560 --> 00:17:39,040
+احنا قلنا راح نقسم العوامل لـchemical and physical
+
+246
+00:17:39,040 --> 00:17:47,060
+agents، بس في نقطة كتير قبل ما نقفز عنها في اللي لو
+
+247
+00:17:47,060 --> 00:17:53,580
+حصلت لقيناها، احنا التعريف الطبي للموت عند البشر توقف
+
+248
+00:17:53,580 --> 00:17:55,200
+القلب أو توقف الدماء
+
+249
+00:17:59,210 --> 00:18:05,750
+بيُعدّ إلى الموت؟ بس بس بس بس بس بس بس بس بس بس بس
+
+250
+00:18:05,750 --> 00:18:10,870
+بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس
+
+251
+00:18:10,870 --> 00:18:13,590
+بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس
+
+252
+00:18:13,590 --> 00:18:13,790
+بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس
+
+253
+00:18:13,790 --> 00:18:14,070
+بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس
+
+254
+00:18:14,070 --> 00:18:14,230
+بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس
+
+255
+00:18:14,230 --> 00:18:20,190
+بس بس بس بس بس بس بس بس بس بس بس بس بس
+
+256
+00:18:20,790 --> 00:18:24,450
+Death is defined by the inability to reproduce
+
+257
+00:18:24,450 --> 00:18:29,390
+مدام مش قادر الميكروبيت يكتر فهو ميت هذا لغرض
+
+258
+00:18:29,390 --> 00:18:36,290
+تعريف الـ disinfection طيب في نقطة كتير مهمة الآن
+
+259
+00:18:36,290 --> 00:18:40,970
+لو اجى واحد وجالنا عرض علينا antiseptic جديد أو
+
+260
+00:18:40,970 --> 00:18:45,290
+disinfectant جديد، الـ disinfectant هذا بدنا نقارنه
+
+261
+00:18:45,290 --> 00:18:50,100
+بدنا نشوف فعاليته، هل هو فعال أو مش فعال، بنعرفش دائماً
+
+262
+00:18:50,100 --> 00:18:55,440
+كلمة نقارن بدها اثنين، بدها اثنين، على الأقل بدنا 
+
+263
+00:18:55,440 --> 00:18:59,460
+واحد معيار وبدنا واحد نقارنه في المعيار، صح؟
+
+264
+00:18:59,460 --> 00:19:03,860
+المعيار اللي موجود عندنا اسمه فينول، الفينول أحد
+
+265
+00:19:03,860 --> 00:19:10,300
+المركبات الأولى
+
+266
+00:19:10,300 --> 00:19:13,140
+اللي استخدمت في عمليات التطهير والـ disinfection
+
+267
+00:19:13,140 --> 00:19:18,540
+وبالتالي أي مركب جديد بنقارنه بمين؟ بالفينول، تعالوا
+
+268
+00:19:18,540 --> 00:19:22,960
+بس لاني بلغة بسيطة عشان مش مجانين نشرح بالضبط تفاصيل
+
+269
+00:19:22,960 --> 00:19:27,900
+كيفية العملية بالتفصيل، لكن نشرح الفكرة الآن، الفينول
+
+270
+00:19:27,900 --> 00:19:35,740
+عند عين تركيز X بيقتل و زمان Y بيقتل عشرة أس ستة
+
+271
+00:19:35,740 --> 00:19:39,980
+من البكتيريا، تمام؟ المادة الجديدة اللي بدي إياها
+
+272
+00:19:39,980 --> 00:19:46,240
+أنا المادة الجديدة اللي بدي إياها بدي أعرف at what
+
+273
+00:19:46,240 --> 00:19:55,190
+concentration، at what time؟ at the same time، at the
+
+274
+00:19:55,190 --> 00:19:59,710
+same time، الناس راح تبقى هنا والكل عشرة أس ستة
+
+275
+00:19:59,710 --> 00:20:06,490
+ايش اللي fixed هنا؟ الزمن؟ الزمن صح؟ وعادل
+
+276
+00:20:06,490 --> 00:20:09,630
+البكتيريا؟ مين ثبتنا هنا المضايقات؟ اللي هو الـ
+
+277
+00:20:09,630 --> 00:20:13,310
+concentration، الـ phenol coefficient هو عبارة عن الـ
+
+278
+00:20:13,310 --> 00:20:18,950
+concentration of phenol على الـ concentration of الـ
+
+279
+00:20:18,950 --> 00:20:25,310
+agent X، العمل الجديد هذا، تخيلوا
+
+280
+00:20:25,310 --> 00:20:30,730
+لو الـ concentration طلعت 20 وأنا عشرين، تمام؟ ايش
+
+281
+00:20:30,730 --> 00:20:35,070
+بتقول الـ coefficient؟ واحد، ايش معنى الحكاية؟ إنه
+
+282
+00:20:35,070 --> 00:20:41,930
+العامل هذا الجديد اللي هو X is comparable نقارنه
+
+283
+00:20:41,930 --> 00:20:45,450
+بمين؟ بالفينول، يعني نفس الكفاءة مع مين؟ مع الفينول
+
+284
+00:20:46,910 --> 00:20:55,090
+point، واحد، ايه الـ coefficient؟ أقل أو أكثر؟ أحسن
+
+285
+00:20:55,090 --> 00:21:03,110
+أو أقل؟ أحسن أو أقل؟ أحسن أو أقل؟ أحسن أو أقل؟
+
+286
+00:21:03,110 --> 00:21:04,830
+أحسن أو أقل؟ أحسن أو أقل؟ أحسن أو أقل؟ أحسن أو
+
+287
+00:21:04,830 --> 00:21:05,850
+أقل؟ أحسن أو أقل؟ أحسن أو أقل؟ أحسن أو أقل؟ أحسن
+
+288
+00:21:05,850 --> 00:21:09,310
+أو أقل؟ أحسن أو أقل؟ أحسن أو أقل؟ أحسن أو أقل؟
+
+289
+00:21:09,310 --> 00:21:09,350
+أحسن أو أقل؟ أحسن أو أقل؟ أحسن أو أقل؟ أحسن أو
+
+290
+00:21:09,350 --> 00:21:18,090
+أقل؟ أحسن أو أقل؟ أحسن، العمل الجديد، لما نقول
+
+291
+00:21:18,090 --> 00:21:22,870
+تركيز أعلى، يعني كفاءة أقل، يبقى لما يكون الـ point
+
+292
+00:21:22,870 --> 00:21:25,630
+... لما يكون الـ coefficient أقل من واحد، يكون
+
+293
+00:21:25,630 --> 00:21:29,810
+العامل أقل من كفاءة الـ phenol، إذا كان أكثر من
+
+294
+00:21:29,810 --> 00:21:34,230
+واحد، العامل تمام، يعني الحاجة إنه الكفاءة الصالحة
+
+295
+00:21:34,230 --> 00:21:35,850
+للعمل الـ major
+
+296
+00:21:45,110 --> 00:21:50,590
+مهم جداً لأنه بيجينا كتير مركبات باستمرار، احنا
+
+297
+00:21:50,590 --> 00:21:56,190
+لازم نحدد فعاليتها عن طريق مقارنة بمين؟ بالفينول
+
+298
+00:21:56,190 --> 00:22:00,430
+ويسمى phenol coefficient، معامل الفينول
+
+299
+00:22:09,870 --> 00:22:12,610
+بيقولنا الـ quantitative measure of this variation
+
+300
+00:22:12,610 --> 00:22:15,750
+is expressed as the phenol coefficient، الكلام اللي
+
+301
+00:22:15,750 --> 00:22:18,110
+حكيناه جاب شويه الـ phenol coefficient اللي هو
+
+302
+00:22:18,110 --> 00:22:24,330
+معامل الـ phenol، لماذا لجأنا لفحص أو لعمل أو
+
+303
+00:22:24,330 --> 00:22:28,930
+لتحديد الـ phenol coefficient؟ بغرض المقارنة، عشان
+
+304
+00:22:28,930 --> 00:22:32,410
+نحدد الـ variation، هحدد اللي هو الـ variation، مهم
+
+305
+00:22:32,410 --> 00:22:35,110
+جدا، نحدد الـ variation عشان نحدد الـ concentration
+
+306
+00:22:35,110 --> 00:22:40,190
+اللي بدي أستخدمها والـ time اللي أنا حامله، Exposure
+
+307
+00:22:40,190 --> 00:22:46,290
+للأسطح اللي أنا بدي أطهرها أو بدي أعقمها، هناك ثلاثة
+
+308
+00:22:46,290 --> 00:22:51,650
+آليات مشهورة جداً في الـ disinfectant والـ
+
+309
+00:22:51,650 --> 00:22:55,130
+antiseptics wherein the agent may kill the
+
+310
+00:22:55,130 --> 00:22:58,330
+microorganism الأولى by cell membrane damage
+
+311
+00:22:58,330 --> 00:23:02,130
+الثانية by protein denaturation، تلاتة by DNA
+
+312
+00:23:02,130 --> 00:23:05,690
+damage أو modification، يعني واحدة من تلاتة يا إما
+
+313
+00:23:05,690 --> 00:23:08,230
+على الـ cell membrane أو على الـ membrane أو على الـ
+
+314
+00:23:08,230 --> 00:23:12,050
+phospholipid، يا إما على البروتيني إنه ... لكن بدكم
+
+315
+00:23:12,050 --> 00:23:16,210
+تلاحظوا نقطة كتير مهمة في هذا الجانب، مختلفة عن الـ
+
+316
+00:23:16,210 --> 00:23:20,050
+antibiotics، الـ antibiotics ... الـ antibiotics
+
+317
+00:23:20,050 --> 00:23:26,370
+بتشتغل على خطوة واحدة، very very specific، الانتيبايوتيك
+
+318
+00:23:26,370 --> 00:23:30,790
+بيشتغل عليها very specific step، يعني مثلاً بيرتبط
+
+319
+00:23:30,790 --> 00:23:35,810
+بالـ 50S-ribosomal subunit وبيمنع الـ translocation
+
+320
+00:23:35,810 --> 00:23:39,670
+للـ amino acids الجديدة، وبالتالي بوقف تصنيع الـ
+
+321
+00:23:39,670 --> 00:23:43,270
+protein، بينما هذا اللي هتقولك modification of
+
+322
+00:23:43,270 --> 00:23:49,750
+proteins، أي protein بيخربه، أي DNA بيخربه، وبالتالي
+
+323
+00:23:49,750 --> 00:23:53,610
+بدنا نفهم إن الميكانيزم هنا مش كتير specific
+
+324
+00:23:54,410 --> 00:23:58,470
+بالإضافة إلى أن بعض المركبات قد تستطيع قتل
+
+325
+00:23:58,470 --> 00:24:03,790
+البكتيريا من خلال أكثر من آلية معينة، وهذا مصطلح مش
+
+326
+00:24:03,790 --> 00:24:09,670
+مذكور معانا الآن، بدنا نكتبه اسمه الـ Oligodynamic
+
+327
+00:24:09,670 --> 00:24:15,590
+Action، قدرنا
+
+328
+00:24:15,590 --> 00:24:20,930
+نكون فهمنا، يعني الـ agent هو واحد قادر على قتل
+
+329
+00:24:21,490 --> 00:24:25,550
+البكتيريا بأكثر من وسيلة، مثلاً بيعمل disruption للـ
+
+330
+00:24:25,550 --> 00:24:27,810
+lipid membrane وبنفس الوقت بيعمل protein
+
+331
+00:24:27,810 --> 00:24:33,630
+denaturation، ايش سمينا هذا العامل؟ الريباندركتشون
+
+332
+00:24:33,630 --> 00:24:37,610
+ليش أنا حابب أتأكد على هذه المعلومة وأذكركم إن الـ
+
+333
+00:24:37,610 --> 00:24:42,450
+antibiotic specific، البكتيريا استطاعت بنجاح إنها
+
+334
+00:24:42,450 --> 00:24:46,490
+تكون resistance لكثير من الـ antibiotic، ليش؟ لأن الـ
+
+335
+00:24:46,490 --> 00:24:50,750
+antibiotic بيأثر على one step، فلو صار الـ mutation
+
+336
+00:24:50,750 --> 00:24:53,850
+فاكرين الـ mutations المحاضرة السابقة؟ لو صار الـ
+
+337
+00:24:53,850 --> 00:24:57,210
+mutation أو it acquired a gene of resistance
+
+338
+00:24:57,210 --> 00:25:00,730
+البكتيريا صارت resistant لمين؟ للـ antibiotic، لكن
+
+339
+00:25:00,730 --> 00:25:05,070
+لحد الآن كده استخدامنا الـ detol، تمام، الـ resistant
+
+340
+00:25:05,070 --> 00:25:08,550
+للـ detol يكاد لا يذكر، الـ resistant للـ chlorine
+
+341
+00:25:08,550 --> 00:25:12,670
+اللي هو الـ chlorine يكاد لا يذكر، لأنه غالباً هو الـ
+
+342
+00:25:12,670 --> 00:25:16,010
+mechanism هي non specific وفي نفس الوقت قد تكون
+
+343
+00:25:16,010 --> 00:25:17,770
+ايه؟ Oligodynamic
+
+344
+00:25:21,210 --> 00:25:23,690
+من المركبات اللي بتعمل disruption لـ cell
+
+345
+00:25:23,690 --> 00:25:29,730
+membrane، الكحول المشهور بالـ Ethanol وفي اسم ثاني
+
+346
+00:25:29,730 --> 00:25:32,550
+اللي لسه مش متقوله، إن احنا هنتذكره كمان شوية اللي
+
+347
+00:25:32,550 --> 00:25:37,930
+هو الـ isopropanol، وفي احنا الـ detergent هو
+
+348
+00:25:37,930 --> 00:25:42,770
+متميز، الـ detergent ليه؟ المنظفات، وقد يتبادر لذهن
+
+349
+00:25:42,770 --> 00:25:46,110
+بعض الناس اللي بيقولون منظفات إنه يخطر على باله
+
+350
+00:25:46,110 --> 00:25:51,040
+الصابون، لأ الصابون ليس له علاقة بالمنظفات، وبتسمعوا
+
+351
+00:25:51,040 --> 00:25:54,880
+انتوا بعض اللي أحياناً في الدعايات، صابون متحرر، صابون
+
+352
+00:25:54,880 --> 00:25:59,720
+Detol على سبيل المثال ويقتل 99.9% من الجراثيم
+
+353
+00:25:59,720 --> 00:26:05,860
+طبعاً هو بيقتل 100% بس الـ 99.9% هذه بيحطوها كمراعاة
+
+354
+00:26:05,860 --> 00:26:09,160
+احنا في الـ micro biology غالباً لا نتدعّى الدقة
+
+355
+00:26:09,160 --> 00:26:14,400
+لأن طريقة تفكيرنا مبنية على assumptions، على
+
+356
+00:26:14,400 --> 00:26:18,250
+افتراضات، ده بيخليهم يشوفوا إن الموت يُعرف بإنه عدم
+
+357
+00:26:18,250 --> 00:26:21,110
+القدرة على البكتيريا لتعمل reproduction، لكن لو أنا
+
+358
+00:26:21,110 --> 00:26:25,050
+أخذت البكتيريا التعبانة اللي هي غير قادرة على الـ
+
+359
+00:26:25,050 --> 00:26:28,770
+reproduction وحطيتها في enrichment media قد تعود
+
+360
+00:26:28,770 --> 00:26:33,350
+يعني قد نحييها مرة أخرى بالتعريف اللي هو الخاطئ
+
+361
+00:26:33,350 --> 00:26:36,130
+اللي موجود في الـ assumption، مش دقيقة، وبالتالي
+
+362
+00:26:36,130 --> 00:26:40,690
+بنحاول قدر الإمكان إنه ما نعطي أيضاً كمان أرقام
+
+363
+00:26:40,690 --> 00:26:45,700
+دقيقة، لما قلنا لكوا PFU، plaque forming unit، ما بنعدش
+
+364
+00:26:45,700 --> 00:26:49,700
+بكتيريا فاج، ولما نعد البكتيريا بنقول cfu، colony
+
+365
+00:26:49,700 --> 00:26:53,300
+forming unit، عشان برضه ما نفترضش إننا بنعد بكتيريا
+
+366
+00:26:53,300 --> 00:26:58,880
+وإننا بنعد وحدة بناء مستمرة، الـ detergent غير عن
+
+367
+00:26:58,880 --> 00:27:05,060
+الصابون، الصابون العادي اللي احنا بنقوله اغسل يديك
+
+368
+00:27:05,060 --> 00:27:09,260
+بالماء والصابون باستمرار، ونصيحة وموجودة في
+
+369
+00:27:09,260 --> 00:27:14,550
+المنزل، موجودة حتى في أكثر الأماكن يعني احتياجها
+
+370
+00:27:14,550 --> 00:27:17,970
+للتطهير والتعقيم، اللي هي المستشفيات أو العيادات
+
+371
+00:27:17,970 --> 00:27:23,690
+الجراحية، إذن هو ليس متحرر الصابون وإنما ... وإنما
+
+372
+00:27:23,690 --> 00:27:27,410
+... لأ مش منظف كمان، خلّيني أشرح هو، لأن كلمة منظف
+
+373
+00:27:27,410 --> 00:27:31,870
+هي مصطلح يطلق على مين؟ على الـ detergent، هي تقوم
+
+374
+00:27:31,870 --> 00:27:34,770
+بـmechanical removal of the microorganism، مش إنه
+
+375
+00:27:34,770 --> 00:27:38,250
+mechanical، يعني بتساعد على إنه البكتيريا اللي على
+
+376
+00:27:38,250 --> 00:27:43,750
+إيدينا تنزل تروح تمام بس ما بيقتلهاش الصابون بينما
+
+377
+00:27:43,750 --> 00:27:47,650
+الـ detergent ممكن يساعد في إزالتها وبيقتلها في نفس 
+
+378
+00:27:47,650 --> 00:27:50,450
+الوجه، فبـ .. هذا مختلف يعني الـ detergent غير عن
+
+379
+00:27:50,450 --> 00:27:53,170
+الصابون بالإضافة إلى الـ phenols اللي أحنا حكينا 
+
+380
+00:27:53,170 --> 00:27:57,790
+عنهم إنه من أقدم المركبات المستخدمة في التطبيق نجل
+
+381
+00:27:57,790 --> 00:28:02,950
+الـ Ethanol Ethanol is widely used to clean the skin 
+
+382
+00:28:02,950 --> 00:28:07,270
+before immunization or venipuncture طبعًا بتعرفين
+
+383
+00:28:07,270 --> 00:28:10,510
+اللي لما بناخدنا الطعام بمسحوناه في قطنة وباخدونا
+
+384
+00:28:10,510 --> 00:28:15,150
+القبره أو لما بنسحب عينة الدم بس الآن بنتكلم على
+
+385
+00:28:15,150 --> 00:28:18,970
+سحب عينة الدم في الـ micro biology بتختلف عن سحب عينة
+
+386
+00:28:18,970 --> 00:28:21,470
+الدم في الـ hematology أو الـ chemical chemistry
+
+387
+00:28:21,470 --> 00:28:29,890
+الكحول الـ 70% تطهير وليس absolute killing
+
+388
+00:28:29,890 --> 00:28:34,490
+صح؟ اللي هو مش ده sterilization أنا بدي أحاول أعمل
+
+389
+00:28:34,490 --> 00:28:38,610
+ما يشبه الـ sterilization في الـ skin لما بدي أعمل
+
+390
+00:28:38,610 --> 00:28:41,530
+blood culture عارفين ليش؟ لأن بالضبط من إن الـ
+
+391
+00:28:41,530 --> 00:28:45,190
+bacteria نمت معي في المختبر نمت معي من الدم وليس 
+
+392
+00:28:45,190 --> 00:28:49,330
+من مين؟ من الجلد لأن الجلد لما احنا دخلنا إلى الدم
+
+393
+00:28:49,330 --> 00:28:54,130
+دخلنا وأخدنا دم لكن عبر مين؟ الجلد فإذا كان في
+
+394
+00:28:54,130 --> 00:28:58,710
+بكتيريا على الجلد هترجع معاها هذا واحد خذوا بالكم
+
+395
+00:28:58,710 --> 00:29:02,650
+الأصل أن العملية هذه تتم حتى في سحب الدم بطريقة
+
+396
+00:29:02,650 --> 00:29:08,710
+روتينية لأن ممكن ندخل أصلاً بكتيريا إلى الدم، الآن
+
+397
+00:29:08,710 --> 00:29:14,570
+عشان نلجأ لهذه العملية، بنضطر نعمل عملية إضافية أو 
+
+398
+00:29:14,570 --> 00:29:19,430
+عملتين إضافيتين، كحول نمسح فيه وعارفين الطريقة من 
+
+399
+00:29:19,430 --> 00:29:24,430
+الـ center للـ outward طريقة الأولابية من المركز إلى
+
+400
+00:29:24,430 --> 00:29:28,880
+اللي هو قلنا إيه إلى الخارج مش من فوق لتحت أو من
+
+401
+00:29:28,880 --> 00:29:34,140
+تحت لفوق يعني إذا كان في أوساخ أوساخ موجودة ننزلها
+
+402
+00:29:34,140 --> 00:29:38,400
+تحت وبعدين نرفعها فوق وكأنك ما عملتيش كأنك 
+
+403
+00:29:38,400 --> 00:29:42,420
+ما عملتيش هيجب أن تكون بشكل دائري لو اللابي بهذا
+
+404
+00:29:42,420 --> 00:29:48,520
+الشكل هذا رقم واحد رقم اثنين بنضيف .. بنضيف اللي 
+
+405
+00:29:48,520 --> 00:29:55,220
+هو الـ أيودين صبغة اليود وبنتركها على اليد لمدة
+
+406
+00:29:55,220 --> 00:30:02,130
+دقيقتين وبعد ما يخلصوا الدقيقتين بنروح واحنا شايلين 
+
+407
+00:30:02,130 --> 00:30:07,650
+الأيودين بقطنة محتوية على 70% الكحول، يبقى كحول،
+
+408
+00:30:07,650 --> 00:30:12,530
+أيودين، وبعدين كحول، هذا بيضمن لأن الأيودين عنده
+
+409
+00:30:12,530 --> 00:30:18,000
+قدرة قتل أكبر بكثير من مين من الكحول بيضمن لي أنه
+
+410
+00:30:18,000 --> 00:30:21,680
+يعني بنسبة تسعة وتسعين بالمئة أكتر من ذلك
+
+411
+00:30:21,680 --> 00:30:24,580
+أنه ما فيش أي مايكرو أورجانزم لأن الأيودين
+
+412
+00:30:24,580 --> 00:30:29,340
+مشهور عنه قدرته على أنه بسموه ده germicidal دلوقت
+
+413
+00:30:29,340 --> 00:30:32,900
+أنه بيقتل البكتيريا والفطريات والفيروسات حتى الـ
+
+414
+00:30:32,900 --> 00:30:37,540
+spores فهو فريد من مين؟ من أنه يكون sterilant مادة
+
+415
+00:30:37,540 --> 00:30:43,880
+معقمة أعتقد أن إحنا حكينا عن الـ 70% وعن الـ 100% و
+
+416
+00:30:43,880 --> 00:30:48,420
+كنا إن الـ 100% يرفع أعلى لسبب
+
+417
+00:30:50,870 --> 00:30:53,210
+لأ أنا بالدولة عاوزة تفضل اللي بتديله مش هو أنا
+
+418
+00:30:53,210 --> 00:30:57,950
+لما زيد الزمن زيدي التركيز بيزيد الزمن؟ لأ عكس هذا
+
+419
+00:30:57,950 --> 00:31:01,590
+الاستثناء وهذا الاستثناء بالعكس لما يزيد التركيز
+
+420
+00:31:01,590 --> 00:31:07,290
+بيقل الزمن طب كيف إنه هي العلاقة أن تتناسب طردي مع
+
+421
+00:31:07,290 --> 00:31:10,730
+سيء في team مش طردي مش طردي عكس طب ماجي لكن
+
+422
+00:31:10,730 --> 00:31:16,090
+التركيز والزمن طردي بين بعض عشان التركيز والزمن
+
+423
+00:31:16,090 --> 00:31:19,390
+.. والزمن .. طرد .. مش قولنا كل ما زادت التركيز
+
+424
+00:31:19,390 --> 00:31:22,470
+.. لأ يا بنتي لأ لأ لأ .. كل ما زادت التركيز قل
+
+425
+00:31:22,470 --> 00:31:26,250
+الزمن .. قل الزمن، هذه علاقة غير علاقة الأولانية،
+
+426
+00:31:26,250 --> 00:31:28,870
+علاقة الأولانية كانت number of survivors، فتناسى
+
+427
+00:31:28,870 --> 00:31:33,770
+التناسب عكسيًا مع العاملين مع بعض، مضلوبين في بعض،
+
+428
+00:31:33,770 --> 00:31:36,770
+التناسب مع عكس طبعًا .. the more .. the more .. the
+
+429
+00:31:36,770 --> 00:31:39,270
+higher the concentration, the less the time is
+
+430
+00:31:39,270 --> 00:31:44,290
+required، يعني علاقة عكسية لكن هذه الاستثناء قلنا أنه
+
+431
+00:31:44,290 --> 00:31:48,830
+الـ concentration لحد معين كل ما ارتفعنا كل ما زادت
+
+432
+00:31:48,830 --> 00:31:52,130
+الكفاءة كل ما زادت الكفاءة لكن لكن هذا إن المائة
+
+433
+00:31:52,130 --> 00:31:57,470
+بالمئة من المركب كفائتها منخفضة إيش السبب؟ أن 
+
+434
+00:31:57,470 --> 00:32:01,430
+الكحول كان يعمل محتاج ماء مائة بالمئة كحول
+
+435
+00:32:01,430 --> 00:32:06,710
+ما فيش ماء قدرته على الـ penetration بتكون أقل وإذا
+
+436
+00:32:06,710 --> 00:32:10,470
+ما اخترقش داخل الخلية ما نحكش مش هعمل destruction
+
+437
+00:32:10,470 --> 00:32:14,250
+للـ membrane ولا الـ proteins هو بيشتغل بالطريقتين طريقة
+
+438
+00:32:14,250 --> 00:32:18,070
+أنه بيعمل disorganization للـ membrane lipid و
+
+439
+00:32:18,070 --> 00:32:21,550
+بيعمل denaturation لمن؟ للـ proteins إذا هو من
+
+440
+00:32:21,550 --> 00:32:25,510
+العوامل اللي بتشتغل oligodynamic action لذلك أنا
+
+441
+00:32:25,510 --> 00:32:30,110
+لحد الآن لم أقرأ بكتيريا مقاومة طبعًا يعني كانت 
+
+442
+00:32:30,110 --> 00:32:34,490
+sensitive قصارة مقاومة أنا مبسوط فيها للكحول لأن 
+
+443
+00:32:34,490 --> 00:32:38,170
+الكحول جدنا بيقضي عليها بطوله non specific لكن
+
+444
+00:32:38,170 --> 00:32:43,540
+تخيلوا لو بيشتغل على one step فقط فيه chance .. فيه
+
+445
+00:32:43,540 --> 00:32:45,760
+chance عالية أن البكتيريا تكون له resistance
+
+446
+00:32:45,760 --> 00:32:49,680
+بتعطيكم مثال بس يعني خارج الإطار عشان برضه الصورة
+
+447
+00:32:49,680 --> 00:32:55,340
+تُضح بشكل جيد هناك حالة بنتسميها Combined Therapy
+
+448
+00:32:55,340 --> 00:32:59,540
+علاج مشترك بمعنى أنه ما نعطي Antibiotic، ما نعطي 2
+
+449
+00:32:59,540 --> 00:33:02,160
+أو 3 أو 4 Antibiotics يعني في مثلًا الـ
+
+450
+00:33:02,160 --> 00:33:05,160
+Tuberculosis، ما نعطيله أربع أنواع من المضاد
+
+451
+00:33:05,160 --> 00:33:11,500
+الحيوية تعني نسميهم ألف A وB وC وD وهي البكتيريا
+
+452
+00:33:12,500 --> 00:33:18,920
+اللي هي Mycobacterium Tuberculosis الآن لو كوّنت
+
+453
+00:33:18,920 --> 00:33:29,880
+resistant لإيه؟ B وC وD يقتلوها، صح؟ يبقى
+
+454
+00:33:29,880 --> 00:33:34,480
+البكتيريا اللي كوّنت resistant لإيه؟ ظهرت على
+
+455
+00:33:34,480 --> 00:33:38,740
+العلم؟ ولا ماتت في مهدها؟ قُتلت في مهدها، لم تنشأ
+
+456
+00:33:38,740 --> 00:33:43,770
+منها خلايا لأن قتلت .. إحنا قتلناهم مع بعض طيب لو
+
+457
+00:33:43,770 --> 00:33:52,690
+جلبنا الـ antibiotic وصحيح أن a,b,c بالإنكوباتور
+
+458
+00:33:52,690 --> 00:33:58,610
+resistant لـ a قتلها مين؟ b وc لكن احتمالية أن 
+
+459
+00:33:58,610 --> 00:34:05,370
+البكتيريا تكون resistant لـ b وc في وجود واحد رابع
+
+460
+00:34:05,370 --> 00:34:11,370
+أو خامس أو سادس بتكون عايشة أقل فكل ما زاد عدد الـ
+
+461
+00:34:11,370 --> 00:34:14,830
+antibiotics اللي أنا بدي أفترض أن كل Antibiotic له
+
+462
+00:34:14,830 --> 00:34:19,450
+mechanism of action فلما يكون الـ disinfectant بيقتل
+
+463
+00:34:19,450 --> 00:34:23,290
+البكتيريا بـ different mechanisms لما لو قورت
+
+464
+00:34:23,290 --> 00:34:26,890
+resistant لميكانيزم هيقتلها بميكانيزم أخرى
+
+465
+00:34:26,890 --> 00:34:30,990
+وبالتالي لن تظهر إلى العالم وتبقى في السوق يعني
+
+466
+00:34:30,990 --> 00:34:35,830
+في الدنيا و بتموت في مهدها وتلصورها فكويس جدًا
+
+467
+00:34:35,830 --> 00:34:38,030
+يكون عندي حاسب ما أقول لكم dynamic action
+
+468
+00:34:42,270 --> 00:34:46,730
+من طبعًا الكحولات المشهورة جدًا في العالم بحاجة
+
+469
+00:34:46,730 --> 00:34:50,530
+اسمها isopropanoid بذكر الأسفلات اللي كتبوها إيزو
+
+470
+00:34:50,530 --> 00:34:54,490
+برضه
+
+471
+00:34:54,490 --> 00:34:59,030
+بيستخدموه بتركيز 70% من ميزاته أنه بيحافظ على
+
+472
+00:34:59,030 --> 00:35:04,550
+رطوبة الجلد أكثر من الـ Ethanol خذوا بالكم النقطة
+
+473
+00:35:04,550 --> 00:35:10,310
+هانفي التعامل في المستشفيات إحنا بننصح أنه بين كل
+
+474
+00:35:10,310 --> 00:35:16,350
+مريض ومريض مثلًا الطبيب أنه يطهر يديه تطهير
+
+475
+00:35:16,350 --> 00:35:19,750
+الأيدان بين .. لو بيشوف له عشرين تلاتين مريض يعني
+
+476
+00:35:19,750 --> 00:35:24,150
+مادي حاجة هيغسل يديه عشرين مرة ما بيخلص آخر نهار
+
+477
+00:35:24,150 --> 00:35:28,650
+اللي هو جلده ناشف متشقق بيصير هو عرض أصلاً للالتهابات
+
+478
+00:35:28,650 --> 00:35:33,710
+الشخص هذا للطبيب أو الـ nurse وبالتالي تطهير
+
+479
+00:35:33,710 --> 00:35:38,340
+والتعقيم أنتج مشكلة جديدة فاضطرينا نحلها بأنه
+
+480
+00:35:38,340 --> 00:35:45,260
+نستخدم مركبات أقل حدة على الجلد مثلًا لازربربنال
+
+481
+00:35:45,260 --> 00:35:50,100
+واحد منهم أو في اسمه alcohol gel الـ alcohol gel
+
+482
+00:35:50,100 --> 00:35:55,840
+بيستخدموه متعالي اللي بيكون عبارة عن جل ونحطه على
+
+483
+00:35:55,840 --> 00:36:02,360
+جل وبعدين هذا بيقلل من اللي هو فقدان الرطوبة في
+
+484
+00:36:02,360 --> 00:36:07,770
+الأيدين وبالتالي بيحافظ على الأيدين رطبة وليس جافة
+
+485
+00:36:07,770 --> 00:36:12,950
+رقم
+
+486
+00:36:12,950 --> 00:36:16,030
+اثنين detergent رقم اثنين هو الـ detergent والـ
+
+487
+00:36:16,030 --> 00:36:19,270
+detergent كما وصفته أنا وليس الصابون وإنما هو
+
+488
+00:36:19,270 --> 00:36:25,070
+يبقى surface active agent عامل له نشاط سطحي و
+
+489
+00:36:25,070 --> 00:36:27,590
+بيسموه surfactant اللي هو المصطلح اللي هنا
+
+490
+00:36:27,590 --> 00:36:33,610
+surfactant تمام؟ بيمتلك hydrophilic و hydrophobic
+
+491
+00:36:33,610 --> 00:36:38,710
+ends مين من المركبات اللي درسناها في البكتيريا
+
+492
+00:36:38,710 --> 00:36:42,150
+عندها hydrophilic و hydrophobic end؟ الـ cell
+
+493
+00:36:42,150 --> 00:36:45,890
+membrane والـ outer membrane تبع الـ gram negative
+
+494
+00:36:45,890 --> 00:36:52,350
+بكتيريا، مضطر لأ؟ طيب لما ييجي surfactant إله
+
+495
+00:36:52,350 --> 00:36:57,770
+hydrophobic end ويتفاعل مع الـ hydrophilic end تبع
+
+496
+00:36:57,770 --> 00:37:02,460
+الـ cell membrane مين أقوى الـ surfactant ولا الـ cell
+
+497
+00:37:02,460 --> 00:37:09,100
+membrane فبيروح اللي هو شادد ممزق الـ cell membrane
+
+498
+00:37:09,100 --> 00:37:12,480
+فاكرين الـ cell membrane كيف وصفناها لما وصفنا زيت
+
+499
+00:37:12,480 --> 00:37:20,200
+زيتون يعني لزج جدًا مرن جدًا رقيق جدًا طبعًا صلاته
+
+500
+00:37:20,200 --> 00:37:25,320
+ضعيفة جدًا وبالتالي من السهل تمزيقه لكن بتلاعب أن 
+
+501
+00:37:25,320 --> 00:37:31,470
+الـ surfactant بيشتغلوا احنا مش قولنا بيدمر الـ cell
+
+502
+00:37:31,470 --> 00:37:36,210
+membrane عبر مين؟ عبر الـ hydrophobic chain و
+
+503
+00:37:36,210 --> 00:37:40,570
+بيرتبط مع الميه في المحيط عبر الـ hydrophobic chain
+
+504
+00:37:40,570 --> 00:37:44,590
+فبيقتل البكتيريا وممكن يزيلها بمعنى آخر زي ما
+
+505
+00:37:44,590 --> 00:37:48,070
+بيشتغل مع الأوساخ، بيرتبط فيها وبيسحبها
+
+506
+00:37:50,720 --> 00:37:54,380
+من أشهر المركبات المشهورة حاجة اسمها Benzyl Konium
+
+507
+00:37:54,380 --> 00:37:59,000
+Chloride وهو يستخدم كـ mild .. mild disinfectant أو 
+
+508
+00:37:59,000 --> 00:38:02,420
+skin disinfectant وهو يتبع إلى ما يسمى بالـ
+
+509
+00:38:02,420 --> 00:38:06,320
+Quaternary Ammonium Sources Quaternary Ammonium
+
+510
+00:38:06,320 --> 00:38:10,260
+Compounds أو Quaternary Ammonium Source Benzyl
+
+511
+00:38:10,260 --> 00:38:15,760
+Konium استخدمت كـ أيش كـ skin العملية اللي بيسميها
+
+512
+00:38:15,760 --> 00:38:17,360
+disinfection
+
+513
+00:38:19,840 --> 00:38:22,640
+المركب التالف في السلسلة اللي هو الفينول
+
+514
+00:38:22,640 --> 00:38:26,560
+والفينول زي ما حكيتها لك هو الـ first disinfectant 
+
+515
+00:38:26,560 --> 00:38:30,620
+used in the operating room by Joseph Lister في الـ
+
+516
+00:38:30,620 --> 00:38:36,720
+1860 أو ستينات القرن التاسع عشر تمانينات القرن ..
+
+517
+00:38:36,720 --> 00:38:41,320
+عفواً تمانينات القرن التاسع عشر Joseph Lister أُعطى
+
+518
+00:38:41,320 --> 00:38:45,080
+الـ credit كله أُعطى يعني الـ .. الـ .. أنه .. أن هو
+
+519
+00:38:45,080 --> 00:38:50,090
+صاحب التعقيم والتطهير في .. في المستشفيات لكن واقع
+
+520
+00:38:50,090 --> 00:38:54,650
+الآن أنا لازم أحكيلكم قصة صغيرة عن أحد الأطباء
+
+521
+00:38:54,650 --> 00:38:59,890
+في أوروبا اللي كان مهتم جداً في دراسة كتب الطب
+
+522
+00:38:59,890 --> 00:39:07,890
+الإسلامية بيقول أنه نسخ الأدوات الجراحية من
+
+523
+00:39:07,890 --> 00:39:12,750
+الكتاب كما هي وعطاها للمصنعين وصنعونا إياها يعني
+
+524
+00:39:12,750 --> 00:39:16,220
+مرسومة بدقة في الكتاب وأعطاها لمثالين صنعوها و
+
+525
+00:39:16,220 --> 00:39:20,620
+أجريت العمليات الجراحية كما أجراها الطبيب الفلاني
+
+526
+00:39:20,620 --> 00:39:25,460
+اللي ذكر اسمه لأ أذكر اسمه أنا فيقول إنه نجحت
+
+527
+00:39:25,460 --> 00:39:28,700
+العملية واستطعت أن أجرّ العملية بالإنواد وبطريقة
+
+528
+00:39:28,700 --> 00:39:33,000
+تماماً لكن المرضى مات بعد العملية بانتفاخات و
+
+529
+00:39:33,000 --> 00:39:35,040
+التهابات ما كانش طبعاً أنهم معروفين شوية يعني
+
+530
+00:39:35,040 --> 00:39:38,720
+بكتيريا أيامها وهو بشكل واضح وتكررت العملية حتى
+
+531
+00:39:38,720 --> 00:39:41,880
+ظنّتوا بيهم في ظنّهم يعني إننا اعتقدت أن هؤلاء
+
+532
+00:39:41,880 --> 00:39:46,060
+كانوا يعني بتحكوا عينا في المعلومات اللي بعثوا فيها يوم
+
+533
+00:39:46,060 --> 00:39:51,240
+يعني بيقرأ فالتفت انتباهه أن أشياء ما كانش يركز
+
+534
+00:39:51,240 --> 00:39:53,780
+عليها زي ما احنا بعضنا حابينها الزبدة بدناش
+
+535
+00:39:53,780 --> 00:40:00,920
+المقدمات وزي ما في كان نكتة بيقول أنه اقطعي جزرة
+
+536
+00:40:00,920 --> 00:40:04,240
+وخيارة ومش عارف شو بتعمل طبيخ وبالآخر بيقول لك إيه
+
+537
+00:40:04,240 --> 00:40:09,980
+بيقول لك اغسل المكونات فهذا
+
+538
+00:40:09,980 --> 00:40:16,070
+عمل العملية عمل عملية كاملة كاملة بحد ذاتها لكن لم
+
+539
+00:40:16,070 --> 00:40:19,430
+يُطبق إجراء كان بيقوم في الطي المسلم كونه مسلم كان
+
+540
+00:40:19,430 --> 00:40:24,550
+يتوضأ قبل إجراء العملية الوضوء قبل إجراء العملية
+
+541
+00:40:24,550 --> 00:40:28,050
+غسيل أيدان وغسيل أيدان عبارة عن تطهير تنظيف مضبوط
+
+542
+00:40:28,050 --> 00:40:31,670
+ولا لأ فجأة طب بتجرب أنا الموضوع هتجرب الموضوع أنه
+
+543
+00:40:31,670 --> 00:40:35,030
+صار يتوضأ وضوء اللي اللي بدي عليه بديه يمشي للآخر
+
+544
+00:40:35,030 --> 00:40:38,730
+وبالفعل بدأت الحالات عنده تتحسن يعني العمليات
+
+545
+00:40:38,730 --> 00:40:42,510
+الجراحية اللي بعد ذلك أن نسبة الوفيات فيها تقل
+
+546
+00:40:42,510 --> 00:40:46,660
+نظراً للالتهابات نبعث العملية اللي حصل على الـ credit
+
+547
+00:40:46,660 --> 00:40:50,820
+اللي هو Joseph Lester هو التاريخ اكتبوه الله يعني
+
+548
+00:40:50,820 --> 00:40:58,240
+هو المنبسطين هذا المركب .. هذا المركب دخل
+
+549
+00:40:58,240 --> 00:41:04,600
+المستشفيات لكن إله خواص سيئة سُمّي كاوية caustic
+
+550
+00:41:04,600 --> 00:41:08,340
+قابضة تمام؟ زي السودا الكاوية تمام؟ caustic soda
+
+551
+00:41:08,340 --> 00:41:13,420
+فهذا الـ .. هذا الـ .. الخاصية جاءت من استخدامه يعني
+
+552
+00:41:13,420 --> 00:41:17,860
+أمر غير مرضي فيه خاصةً بعد ظهور مركبات أخرى في
+
+553
+00:41:17,860 --> 00:41:22,240
+الخدمة أحد المركبات اللي اشتقت دمجوا مركبين
+
+554
+00:41:22,240 --> 00:41:29,520
+الفينول والكلور فهو يبقى هكسا كلورفين
+
+555
+00:41:29,520 --> 00:41:33,580
+كلورفين اللي هو الكلورين مع فينول which is a
+
+556
+00:41:33,580 --> 00:41:36,980
+biphenol with six chlorine atoms is used in
+
+557
+00:41:36,980 --> 00:41:40,200
+germicidal soaps حطوها لما .. لما عملوا الصابون
+
+558
+00:41:40,200 --> 00:41:44,770
+ودخلوا الصابون لحاله التركيب مش deserved ولا قاتل
+
+559
+00:41:44,770 --> 00:41:47,970
+للبكتيريا، لكن احنا بنشوف في الـ .. في الـ .. في الـ
+
+560
+00:41:47,970 --> 00:41:51,710
+.. في السوق الآن أنه في germicidal soups يضاف لها
+
+561
+00:41:51,710 --> 00:41:56,790
+مركبات تمام ليجعلها قاتلة، زي الـ Detol soup لكن
+
+562
+00:41:56,790 --> 00:42:01,210
+بيقولنا .. بيقولنا أنه هناك قلق من أنه إله neuro
+
+563
+00:42:01,210 --> 00:42:06,270
+toxicity هذا المركب أظهر أو بعض الدراسات أظهرت أنه
+
+564
+00:42:06,270 --> 00:42:09,870
+ممكن يكون إله neuro toxicity فأوقف برضه استخدام
+
+565
+00:42:10,540 --> 00:42:15,680
+أنشأ ومركب جديد من الفينول another phenol derived
+
+566
+00:42:15,680 --> 00:42:21,500
+from this grisol وهو يبقى مثالفينول مثالفينول the
+
+567
+00:42:21,500 --> 00:42:24,880
+active ingredient in lysol اللي هو شبيه بالديترون
+
+568
+00:42:24,880 --> 00:42:30,140
+شبيه بالديترون Phenols not only damage membranes
+
+569
+00:42:30,140 --> 00:42:35,280
+but also damage من البروتين يبقى الآن أن مركبين
+
+570
+00:42:35,280 --> 00:42:40,150
+عرفناهم اللي هم الفينولات والكحولات تمام عندهم نقطة
+
+571
+00:42:40,150 --> 00:42:43,150
+على الـ destruction للـ cell membrane بالإضافة إلى
+
+572
+00:42:43,150 --> 00:42:50,750
+الـ proteins الآن بنجي للعنوان الثاني في الـ
+
+573
+00:42:50,750 --> 00:42:53,310
+mechanism of actions للـ disinfectant و الـ
+
+574
+00:42:53,310 --> 00:42:57,570
+antiseptic اللي هو الـ denaturation أو الـ
+
+575
+00:42:57,570 --> 00:43:00,510
+modification of proteins عندنا chlorine, iodine,
+
+576
+00:43:00,590 --> 00:43:02,690
+heavy metals, hydrogen peroxide عندنا
+
+577
+00:43:02,690 --> 00:43:06,450
+formaldehyde و glutaraldehyde Ethylene Oxide اللي
+
+578
+00:43:06,450 --> 00:43:09,770
+مرّ معنا في أول المحاضرة لما نتكلم عن غاز
+
+579
+00:43:09,770 --> 00:43:14,370
+المستخدم في التعقيم وفي Acid and Alkali طبعاً الـ
+
+580
+00:43:14,370 --> 00:43:17,630
+Acid and Alkali ممكن تشتغل بأكثر من طريقة الـ pH
+
+581
+00:43:17,630 --> 00:43:21,590
+نفسه يعمل suppression أو أنه هو نفسه يكون عنده
+
+582
+00:43:21,590 --> 00:43:24,670
+mechanism of killing زي البنزوات الـ sodium أن
+
+583
+00:43:24,670 --> 00:43:28,350
+تستخدمها كمادة حافظة في بعض المربيات المنزلية اللي
+
+584
+00:43:28,350 --> 00:43:32,110
+عملوها أو بعض المخللات المنزلية بتعملوا كرامة
+
+585
+00:43:32,110 --> 00:43:34,190
+الفيروس بتستخدم اللي هو الـ Sodium Benzoate
+
+586
+00:43:34,190 --> 00:43:37,710
+ويشتروها من الصيدليات كمادة بيقولوا عنها
+
+587
+00:43:37,710 --> 00:43:42,810
+الـ بنزوات، تمام؟ الـ Sodium وهي مادة حافظة تنحل
+
+588
+00:43:42,810 --> 00:43:46,730
+وتكون Benzoic Acid، في الآخر هي إيه؟ عبارة عن
+
+589
+00:43:46,730 --> 00:43:54,110
+Acid، Organic Acid طيب، الـ chlorine و الـ Iodine
+
+590
+00:43:54,110 --> 00:44:00,600
+بينتموا إلى الـ halogen's both have يعني
+
+591
+00:44:00,600 --> 00:44:04,220
+antibacterial أو antimicrobial effect لكن
+
+592
+00:44:04,220 --> 00:44:08,920
+استخدامتهم مختلفة مثلاً أنتم تشربوا الماء مخلوط
+
+593
+00:44:08,920 --> 00:44:12,360
+بمين؟ بالكلورين بس أنتم مش تشربوا الماء مخلوط
+
+594
+00:44:12,360 --> 00:44:15,800
+بالايودين صح ولا لأ؟ هذا يستخدم لغرض وهذا يستخدم
+
+595
+00:44:15,800 --> 00:44:21,100
+لأغراض مختلفة تعالوا نطلع على الكلورين الكلورين is
+
+596
+00:44:21,100 --> 00:44:26,020
+used as a disinfectant to purify the water supply
+
+597
+00:44:26,020 --> 00:44:30,390
+and to treat swimming pools أنتم تعرفوا أن في قطاع
+
+598
+00:44:30,390 --> 00:44:34,730
+غزة we are blessed by a plenty of areas where
+
+599
+00:44:34,730 --> 00:44:38,770
+swimming pools are common يعني الآن صار فينا مناطق
+
+600
+00:44:38,770 --> 00:44:42,050
+متنوعة مختلفة حتى في بعض المنازل صار فيها swimming
+
+601
+00:44:42,050 --> 00:44:45,870
+pools اللي عنده قطعة أرض صغيرة بيعمله قط .. لكن
+
+602
+00:44:45,870 --> 00:44:51,790
+للأسف كثير من هؤلاء الناس ما .. مش فاهمين إيش يعني
+
+603
+00:44:51,790 --> 00:44:59,090
+swimming pool different persons بيدخلوا المكان هذا
+
+604
+00:44:59,090 --> 00:45:04,930
+مستوى النظافة عندهم غير معروف للجميع تمام؟ من مصادر
+
+605
+00:45:04,930 --> 00:45:10,150
+مختلفة قد تتلوث المياه هناك فلابد من continuous
+
+606
+00:45:10,150 --> 00:45:15,170
+change أو disinfection للـ water فالكلورين أحد أهم
+
+607
+00:45:15,170 --> 00:45:20,430
+المركبات كمان شغل مهمة احنا المياه في قطاع غزة
+
+608
+00:45:20,430 --> 00:45:25,240
+مصدرها الأساسي المياه الجوفية غالباً المياه الجوفية
+
+609
+00:45:25,240 --> 00:45:30,640
+لأن الماء بينزل وبتفلتر وبيصل لخزان الجوفي، بتفلتر
+
+610
+00:45:30,640 --> 00:45:34,380
+عبر سبعين، ستين، خمسين متر من الرمل والطين و
+
+611
+00:45:34,380 --> 00:45:40,360
+الحصر، فبينزل pure تحت، نقي جداً، لكن لما بنسحب و
+
+612
+00:45:40,360 --> 00:45:45,060
+احنا وبنوزع في الشبكة، تمام؟ ما بنعرفش الشبكة إيش
+
+613
+00:45:45,060 --> 00:45:52,180
+فيها طب روحه هم حاطين dosage من الـ chlorine بحيث
+
+614
+00:45:52,180 --> 00:45:56,900
+يصلك أنت في المنزل تمام في ما يسمى بالـ residual
+
+615
+00:45:56,900 --> 00:46:02,120
+chlorine الـ residual يعني كمية المتبقية مسموح
+
+616
+00:46:02,120 --> 00:46:06,500
+بها، مسموح بها يعني غير ضارة للإنسان حتى يقتل أي
+
+617
+00:46:06,500 --> 00:46:10,080
+ميكروبات قد تعلق في البكتيريا من الشبكة طيب بلاش
+
+618
+00:46:10,080 --> 00:46:14,050
+احنا هنا ground water نظيف طب تعالوا نروح على دولة
+
+619
+00:46:14,050 --> 00:46:16,970
+أخرى معتمدة على الـ surface water ولا افترض نهر
+
+620
+00:46:16,970 --> 00:46:20,970
+النيل ونهر النيل يمكن شوفته أنتم بعض المناظر هنا
+
+621
+00:46:20,970 --> 00:46:24,510
+الـ youths بتكون طافية لحيوانات في ناس بتغسل على
+
+622
+00:46:24,510 --> 00:46:29,950
+ضفاف النهر يعني بيحتوي على كم كبير من الملوثات
+
+623
+00:46:29,950 --> 00:46:35,970
+فكيف لنا أن نقول أن هذا الماء صار للشرب؟ بدنا
+
+624
+00:46:35,970 --> 00:46:40,150
+ندخله .. بدنا نشيل المواد العالقة فيه وهذه خطوة
+
+625
+00:46:40,150 --> 00:46:42,550
+بيبدأوا فيها الأخوة البيئيين وبعدين نعمله 
+
+626
+00:46:42,550 --> 00:46:46,130
+disinfection .. وبعدين نعمله disinfection ب ..
+
+627
+00:46:46,130 --> 00:46:49,850
+برضه الـ chlorine .. بسبب الـ chlorine أداء مهمة جدا
+
+628
+00:46:49,850 --> 00:46:54,630
+جدا .. كمان شغلة الـ waste water .. المياه العادمة
+
+629
+00:46:54,630 --> 00:46:59,250
+.. لما احنا نتخلص منها في البحر .. الأصل قبل أن 
+
+630
+00:46:59,250 --> 00:47:03,650
+نتخلص منها في البحر .. تمام .. ينحط معاها برضه الـ
+
+631
+00:47:03,650 --> 00:47:07,860
+chlorine عشان يقتل الميكروبات لإن احنا .. احنا
+
+632
+00:47:07,860 --> 00:47:11,480
+بنتخلص من المجاري في البحر و في الصيف بروح نلحق
+
+633
+00:47:11,480 --> 00:47:20,460
+للمجاري تبعتنا هذه طب عنواني الأمر مابيخلاش من بعض
+
+634
+00:47:20,460 --> 00:47:23,760
+.. مابيخلاش من بعض الـ .. الـ يعني خدنا نقول الأشياء
+
+635
+00:47:23,760 --> 00:47:27,900
+الإشبارية معظم دول العالم بتتخلص مني فهي تبعتها في
+
+636
+00:47:27,900 --> 00:47:31,220
+الأجسام بتاعة المائية .. الأجسام المائية في البحار
+
+637
+00:47:32,260 --> 00:47:36,320
+حتى نقلل الضرر، بنقوم بعملته اللي هو الـ
+
+638
+00:47:36,320 --> 00:47:39,040
+disinfection أو المعالجة، بيسموها المعالجة
+
+639
+00:47:39,040 --> 00:47:42,680
+الثالثية وبعض الدول الآن أصبحت الـ wastewater
+
+640
+00:47:42,680 --> 00:47:48,540
+بالنسبة لكنزي strategy ممنوع يتضخه في البحر وإنما
+
+641
+00:47:48,540 --> 00:47:53,000
+يجب أن نستخدم لرأي المزرعة تونس دولة رائدة من
+
+642
+00:47:53,000 --> 00:47:56,300
+الدول الرائدة اللي بدأت تجربة رائدة فيه إنها تسقي
+
+643
+00:47:56,300 --> 00:48:01,540
+الحدائق العامة باستخدام المياه المعالجة معالجة
+
+644
+00:48:01,540 --> 00:48:07,580
+ثالثية يعني فيها نزال العلق والبكتيرين نكمل المرة
+
+645
+00:48:07,580 --> 00:48:08,380
+القادمة إن شاء الله 
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/Wu7cQmVXlBc_raw.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/Wu7cQmVXlBc_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..68f65f616d51db8044583c5950482578c1293839
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/Wu7cQmVXlBc_raw.srt
@@ -0,0 +1,2588 @@
+1
+00:00:20,920 --> 00:00:23,120
+السلام عليكم ورحمة الله وبركاته بسم الله الرحمن
+
+2
+00:00:23,120 --> 00:00:27,120
+الرحيم أنواع المحاضرة اليوم هو ال sterilization
+
+3
+00:00:27,120 --> 00:00:31,760
+and disinfection وهذا الموضوع يأتي بعد سلسلة
+
+4
+00:00:31,760 --> 00:00:36,560
+مواضيع كلها كانت مركزة على تركيب الخلية ومن ثم
+
+5
+00:00:36,560 --> 00:00:40,820
+كيفية تنمية الخلية وما هي العوامل اللي تؤثر على
+
+6
+00:00:40,820 --> 00:00:45,420
+النمو بدل منمي البكتيريا الحقيقة أنا ذكرت في نهاية
+
+7
+00:00:45,420 --> 00:00:49,560
+المحاضرة السابقةإنه ليس من الصعب تنمية البكتيريا
+
+8
+00:00:49,560 --> 00:00:54,020
+وإنما اللي صعب أو أصعب هو كيف أنا أتخلص من
+
+9
+00:00:54,020 --> 00:01:00,580
+البكتيريا واليوم هنبدأ الموضوع هذا ببعض المصطلحات
+
+10
+00:01:00,580 --> 00:01:05,460
+ومن ثم بعض الأدوات وبعض التقنيات المستخدمة في
+
+11
+00:01:05,460 --> 00:01:07,960
+السيطرة على ال micro organism أو ال control of
+
+12
+00:01:07,960 --> 00:01:13,950
+microorganismبنعرف إنه من خلال التعريف اللي مورده
+
+13
+00:01:13,950 --> 00:01:17,350
+لنا sterilization إنه مصطلح مطلق كلمة مصطلح مطلق
+
+14
+00:01:17,350 --> 00:01:22,210
+يعني لا يقبل الاستثناء بمعنى إنه بقولنا هنا is the
+
+15
+00:01:22,210 --> 00:01:26,810
+killing or removal of all microorganismsكلمة all
+
+16
+00:01:26,810 --> 00:01:31,170
+تعني المتبقاش zero وبالتالي هو بالفعل absolute
+
+17
+00:01:31,170 --> 00:01:35,510
+term بدنا دايما نميزه يعني مداش نخلط فيهم الأيام و
+
+18
+00:01:35,510 --> 00:01:40,310
+نقول sterilization و disinfection و disinfection
+
+19
+00:01:40,310 --> 00:01:44,550
+هم نفس المصطلح لأ باختلفوا عن بعض اختلاف كبير ال
+
+20
+00:01:44,550 --> 00:01:49,330
+sterilization absolute term بينما disinfection لأ
+
+21
+00:01:49,330 --> 00:01:50,730
+بيقبل ال compromise
+
+22
+00:01:54,260 --> 00:01:56,960
+إذا بتلاحظوا ال sterilization is usually carried
+
+23
+00:01:56,960 --> 00:02:01,980
+out using autoclaving ال autoclaving أو ال
+
+24
+00:02:01,980 --> 00:02:05,400
+autoclave هو أدا من الأدوات اللي كلكم بتعرفوها في
+
+25
+00:02:05,400 --> 00:02:09,720
+المنزل اللي هي عبارة عن pressure cooker أو تنجرة
+
+26
+00:02:09,720 --> 00:02:15,240
+الضغط احنا بنعرف أنه نقطة غليان الماء هي 100 درجة
+
+27
+00:02:15,240 --> 00:02:19,360
+100 أوي 100 درجة 100 أوي لو سخننا نص ساعة ساعة
+
+28
+00:02:19,360 --> 00:02:25,290
+ساعتين تلاتة في الآخر بتضلها 100100 درجة مئوية إذا
+
+29
+00:02:25,290 --> 00:02:30,670
+كيف نحصل على درجة أعلى من تلك الدرجة؟ عن طريق ضغط
+
+30
+00:02:30,670 --> 00:02:37,810
+بخار الماء نضغط البخار فترتفع الحرارة بمقدار الضغط
+
+31
+00:02:37,810 --> 00:02:42,970
+يعني بمثال مولود اليوم على السطولة لو كان الضغط 15
+
+32
+00:02:42,970 --> 00:02:47,650
+PSI 15 PSI هو المصطلح الأكثر شروعا في الاستخدام
+
+33
+00:02:47,650 --> 00:02:52,440
+وهو عبارة عن pound per square inchsquare inch اللي
+
+34
+00:02:52,440 --> 00:02:55,980
+هي بوصة مربعة وال bound هو راطلينجيز يعني هي من
+
+35
+00:02:55,980 --> 00:03:01,180
+قياسة إذا كانت ضغط 15 psi بتكون درجة حرارة الماء
+
+36
+00:03:01,180 --> 00:03:08,440
+121 درجة مائوية لو احتفظنا بهذه الدرجة و هذا الضغط
+
+37
+00:03:08,440 --> 00:03:17,940
+لمدة 15 دقيقة تمام هنا نحصل على تعقيم بمعنى أن كل
+
+38
+00:03:17,940 --> 00:03:24,330
+المخلوقاتالطبية اللى بنعرفها تموت عند هذه الدرجة
+
+39
+00:03:24,330 --> 00:03:29,910
+لكن بدنا نكون متأكدين أنه بالفعل الدرجة اللى
+
+40
+00:03:29,910 --> 00:03:32,110
+قدامنا اللى على ال .. اللى .. اللى على ال counter
+
+41
+00:03:32,110 --> 00:03:36,790
+إنها 121 وإن الضغط صحيح وإن ال timing بدأ .. متى
+
+42
+00:03:36,790 --> 00:03:41,570
+بدأ ال timing؟ بعد ما وصلت الحرارة ل 121 درجة
+
+43
+00:03:41,570 --> 00:03:45,790
+مئوية يعني مش من أول العملية لأ بعد وصول العملية
+
+44
+00:03:45,790 --> 00:03:50,940
+إلى 121 درجة مئوية ثلاث ألقاب سهلينمية واحد عشرين
+
+45
+00:03:50,940 --> 00:03:52,820
+خمستاشر خمستاشر خمستاشر خمستاشر خمستاشر خمستاشر
+
+46
+00:03:52,820 --> 00:03:55,560
+خمستاشر خمستاشر خمستاشر خمستاشر خمستاشر خمستاشر
+
+47
+00:03:55,560 --> 00:03:58,980
+خمستاشر
+
+48
+00:03:58,980 --> 00:04:02,060
+خمستاشر
+
+49
+00:04:02,060 --> 00:04:04,100
+خمستاشر خمستاشر خمستاشر خمستاشر خمستاشر خمستاشر
+
+50
+00:04:04,100 --> 00:04:04,400
+خمستاشر خمستاشر خمستاشر خمستاشر خمستاشر خمستاشر
+
+51
+00:04:04,400 --> 00:04:04,740
+خمستاشر خمستاشر خمستاشر خمستاشر خمستاشر خمستاشر
+
+52
+00:04:04,740 --> 00:04:05,620
+خمستاشر خمستاشر خمستاشر خمستاشر خمستاشر خمستاشر
+
+53
+00:04:05,620 --> 00:04:17,900
+خمستاشر خمستاشر خمستاشر خمستاشر خمستاشر
+
+54
+00:04:17,900 --> 00:04:23,410
+خمالشاشة الطبية على سبيل المثال لكن هناك أدوات قد
+
+55
+00:04:23,410 --> 00:04:29,370
+تتدمر بالتعقيم بمعنى بروتين لو بنعقم ال albumin
+
+56
+00:04:29,370 --> 00:04:32,770
+تبع البيض وحطيناها في ال autoclave درجة الحرارة
+
+57
+00:04:32,770 --> 00:04:36,930
+هتكون إيه؟ عالي جدا بحيث إن ال albumin هيصير له
+
+58
+00:04:36,930 --> 00:04:41,090
+coagulation بيبطل السائل بيصير صلب طب في هذه
+
+59
+00:04:41,090 --> 00:04:44,010
+الحالة أنا بدّي البروتين هذا بدّي ال albumin
+
+60
+00:04:44,010 --> 00:04:47,170
+بدّيها سائل ليش هي صلب؟ يبقى ماقدرش أعقمه بال
+
+61
+00:04:47,170 --> 00:04:50,410
+autoclaveيبقى الـ autoclave على الرغم من شيوعه
+
+62
+00:04:50,410 --> 00:04:55,430
+وانتشاره وكثرة استخداماته اللي انه غير صالح لكل
+
+63
+00:04:55,430 --> 00:05:00,250
+الأغراض اللي احنا من أجلها بنعقم الأشياء فبقولنا
+
+64
+00:05:00,250 --> 00:05:04,050
+مثلا surgical instruments that may rust رست يعني
+
+65
+00:05:04,050 --> 00:05:09,430
+بتصضي في وجود اللي هو البخار المال، لا يستطيعون
+
+66
+00:05:09,430 --> 00:05:13,630
+يعقينه ال autoclave جهاز لابتوب زي هذا لو صار عليه
+
+67
+00:05:13,630 --> 00:05:17,330
+spill، bacterial spill وانكبت عليه مزرعة بكتيريا،
+
+68
+00:05:17,330 --> 00:05:21,810
+وبدأ نعقمهبنفع نحطه في الوتو كليف؟ طيب بنفع نضيل
+
+69
+00:05:21,810 --> 00:05:26,390
+عليه كلور؟ مانفعش يبجي بأحسن وسيلة تعقيمه في هذه
+
+70
+00:05:26,390 --> 00:05:31,350
+الحالة للغاز إنه نجيب مادة غازية قاتلة الغاز
+
+71
+00:05:31,350 --> 00:05:36,850
+بيخترق الأشياء دون أن يحدث بلل أو wet وبالتالي
+
+72
+00:05:36,850 --> 00:05:41,070
+بتظل الدوائل الكهربائية شغالة إذن فين دي أشكال
+
+73
+00:05:41,070 --> 00:05:44,730
+وأنواع متختلفة من اللي هو المواد المستخدمة له ال
+
+74
+00:05:44,730 --> 00:05:48,530
+sterilization ألا بالله حاجة ذكرت واحد تعريفرقم
+
+75
+00:05:48,530 --> 00:05:53,550
+اتنين ذكرت القالية اللى بتتم من خلالها the math
+
+76
+00:05:53,550 --> 00:05:57,750
+اللى زى اللى هو ال autoclaving ثم اتحدثت على بداء
+
+77
+00:05:57,750 --> 00:06:02,890
+الأخرى اتحدثت عن مثلا ال ethane oxide اللى هو
+
+78
+00:06:02,890 --> 00:06:06,930
+الجاز فى هذه الحالة وفى كمان حالة تالتة موجودة على
+
+79
+00:06:06,930 --> 00:06:10,610
+اللابحة اللى هى ال filtrationأنا أقول كده بشري ال
+
+80
+00:06:10,610 --> 00:06:14,070
+البيومين صح؟ ال البيومين إذا أنا بديها protein
+
+81
+00:06:14,070 --> 00:06:17,870
+السائل كما هو بمرره عبر filter ال filter هذا
+
+82
+00:06:17,870 --> 00:06:21,310
+مساماته اللي هتسبح بمرور البكتيريا و الفيروسات
+
+83
+00:06:21,310 --> 00:06:24,990
+وبالتالي كل ما يمر أو لما السائل يمر عبره
+
+84
+00:06:24,990 --> 00:06:30,050
+البكتيريا و الفيروسات تحجز إلى الأعلى و بنزل
+
+85
+00:06:30,050 --> 00:06:33,910
+نستقبل السائل في انبوبة معقمة وبالتالي سيجعلنا
+
+86
+00:06:33,910 --> 00:06:38,380
+تعقيم بال filtration إذن هناك وسائل مختلفةبس
+
+87
+00:06:38,380 --> 00:06:40,960
+بيتلاحظوا شغل من اللوحة هذه قبل ما ننتقل للوحة
+
+88
+00:06:40,960 --> 00:06:44,920
+تانية ان هناك من الوسائل اللي ذكرناها وسائل
+
+89
+00:06:44,920 --> 00:06:49,000
+ميكانيكية زي ال filtration وسائل فيزيائية زي
+
+90
+00:06:49,000 --> 00:06:54,240
+الحرارة وسائل كيميائية زي اللي هو الغاز إذا غالبا
+
+91
+00:06:54,240 --> 00:06:58,220
+هنقسم ال methods إلى physical and chemical methods
+
+92
+00:06:58,220 --> 00:07:02,240
+لكن لسه ما خلصنا من التعريفات وبالتالي بدنا نكمل
+
+93
+00:07:02,240 --> 00:07:06,870
+موضوع التعريفاتأنا من البداية قاعد بحذر من الخلط
+
+94
+00:07:06,870 --> 00:07:11,350
+ما بين المصطلحين اللي هو sterilization اللي هو ال
+
+95
+00:07:11,350 --> 00:07:12,490
+sterilization اللي هو ال sterilization اللي هو ال
+
+96
+00:07:12,490 --> 00:07:13,710
+sterilization اللي هو ال sterilization اللي هو ال
+
+97
+00:07:13,710 --> 00:07:14,690
+sterilization اللي هو ال sterilization اللي هو ال
+
+98
+00:07:14,690 --> 00:07:15,710
+sterilization اللي هو ال sterilization اللي هو ال
+
+99
+00:07:15,710 --> 00:07:16,130
+sterilization اللي هو ال sterilization اللي هو ال
+
+100
+00:07:16,130 --> 00:07:16,550
+sterilization اللي هو ال sterilization اللي هو ال
+
+101
+00:07:16,550 --> 00:07:16,950
+sterilization اللي هو ال sterilization اللي هو ال
+
+102
+00:07:16,950 --> 00:07:17,450
+sterilization اللي هو ال sterilization اللي هو ال
+
+103
+00:07:17,450 --> 00:07:17,990
+sterilization اللي هو ال sterilization اللي هو ال
+
+104
+00:07:17,990 --> 00:07:17,990
+sterilization اللي هو ال sterilization اللي هو ال
+
+105
+00:07:17,990 --> 00:07:18,150
+sterilization اللي هو ال sterilization اللي هو ال
+
+106
+00:07:18,150 --> 00:07:18,150
+sterilization اللي هو ال sterilization اللي هو ال
+
+107
+00:07:18,150 --> 00:07:18,770
+sterilization اللي هو ال sterilization اللي هو ال
+
+108
+00:07:18,770 --> 00:07:22,290
+sterilization اللي هو ال sterilization اللي هو ال
+
+109
+00:07:22,290 --> 00:07:25,210
+sterilization اللي هو ال sterilization اللي هو ال
+
+110
+00:07:25,210 --> 00:07:29,310
+sterilization اللي هو ال sterilization اللي
+
+111
+00:07:36,910 --> 00:07:40,610
+الان في مصطلح او مصطلحين بدنا ننتبه لهم جيدا
+
+112
+00:07:40,610 --> 00:07:47,190
+disinfectants ومصطلح تاني اسمه antiseptic انت
+
+113
+00:07:47,190 --> 00:07:53,570
+هتكلم عن مين؟ disinfection ال antiseptic او ال
+
+114
+00:07:53,570 --> 00:08:00,390
+disinfectant بيحمل نفس التعريف مادة تستخدم لمين؟
+
+115
+00:08:02,000 --> 00:08:06,960
+لكن لماذا ميزنا بينهم بالاسم ال disinfectants
+
+116
+00:08:06,960 --> 00:08:11,360
+تستخدم لل inanimate objects يعني الأسطح الغير حية
+
+117
+00:08:11,360 --> 00:08:16,120
+بينما ال antiseptic بنستخدمه للأسطح الحية و لنفترض
+
+118
+00:08:16,120 --> 00:08:20,600
+الجل يعني الكحول اللي بنستخدمه اللي هو ال 70%
+
+119
+00:08:20,600 --> 00:08:25,200
+alcohol اللي بنستخدمه ل skin disinfection هذا
+
+120
+00:08:25,200 --> 00:08:29,630
+عبارة عن عياش antisepticلو استخدمته لمسح الطاولة
+
+121
+00:08:29,630 --> 00:08:32,830
+أو تطير الطاولة بيكون اسمها يبقى هو اللي انا
+
+122
+00:08:32,830 --> 00:08:37,870
+استخدمناها لغرضين مختلفين فأخد اسمين مختلفين أخد
+
+123
+00:08:37,870 --> 00:08:40,510
+مرة Antiseptic لما استخدمته على الجلد و أخد اسم
+
+124
+00:08:40,510 --> 00:08:46,290
+تاني اعتقده من الصورة و أضحى في هذا المجال طيب ما
+
+125
+00:08:46,290 --> 00:08:49,970
+الذي يجعلنا نقرر استخدام مادة على الجلد و مادة
+
+126
+00:08:49,970 --> 00:08:54,070
+أخرى على الأسطح عشان تقول انت ما تأثرش على اللي
+
+127
+00:08:54,070 --> 00:08:58,290
+أنا بدأت .. يعني بدأ أطهرهيعني لو .. لو افترضنا
+
+128
+00:08:58,290 --> 00:09:00,950
+انه مية النار .. مية النار اللي هي الhydrochloric
+
+129
+00:09:00,950 --> 00:09:05,130
+acid قاطلة للبكتيريا، اتميزاش نحطها على الجلد؟
+
+130
+00:09:05,130 --> 00:09:07,070
+احنا طبعا .. احنا مستخدمهااش، لكن بستخدم
+
+131
+00:09:07,070 --> 00:09:10,710
+الchlorine على سمك متر، الchlorine معروف عنه قاطل
+
+132
+00:09:10,710 --> 00:09:15,870
+جيد للبكتيريا، لكن بيعمل skin irritation، يعني
+
+133
+00:09:15,870 --> 00:09:19,350
+تهيش فيه الجلد، وقد يعمل skin burn، كمان حرق في
+
+134
+00:09:19,350 --> 00:09:23,170
+الجلد، طب نستخدموش على الجلد، بستخدموه على .. على
+
+135
+00:09:23,170 --> 00:09:27,800
+أسطحالأيودين أقل حد من مين من الكلورين، مع أنه
+
+136
+00:09:27,800 --> 00:09:31,340
+التنوع من نفس الفصيلة الكلورين .. أقل الهالوجينس،
+
+137
+00:09:31,340 --> 00:09:36,580
+لكن نستخدمه على مين؟ على الجلب، ما نستخدموه على
+
+138
+00:09:36,580 --> 00:09:42,680
+الأسطح لأنه بيترك stain، أثر، مظبوط؟ فهو mild على
+
+139
+00:09:42,680 --> 00:09:45,980
+ال skin و سيء لمين؟ لأ، الأسطح، إذا اللي بيقالينا
+
+140
+00:09:45,980 --> 00:09:49,580
+نقرر إنه نستخدمه لسه infected أو unseptic هو ال
+
+141
+00:09:49,580 --> 00:09:52,660
+property استبعته، هل هو toxic، هل بيعمل stain،
+
+142
+00:09:52,660 --> 00:09:56,550
+أعطيكم مثال تالتبتعرفوا ال crystal violet ال
+
+143
+00:09:56,550 --> 00:10:00,610
+crystal violet اللي هو اسم تاني جينشان فيولت هذا
+
+144
+00:10:00,610 --> 00:10:04,370
+معروف عنه المركب انه عنده قدرة على قتل البكتيريا
+
+145
+00:10:04,370 --> 00:10:08,610
+واستخدمناه في وسط اسم الماكونكي بتعرف الماكونكي؟
+
+146
+00:10:08,610 --> 00:10:14,230
+الماكونكي selective media أضفنا عليه crystal
+
+147
+00:10:14,230 --> 00:10:18,250
+violet حتى نمنع ال gram-bostig بكتيريا يبقى هو
+
+148
+00:10:18,250 --> 00:10:22,630
+القاتل لمين؟ لل gram-bostigطيب ليش ما نستخدمه احنا
+
+149
+00:10:22,630 --> 00:10:26,450
+في ال disinfection و ال antiseptic؟ و للأسف
+
+150
+00:10:26,450 --> 00:10:30,270
+ماستخدموهش للغرضين لأنه بيترك stain لو انا
+
+151
+00:10:30,270 --> 00:10:33,950
+استخدمته على أيدي بدي أسبوع أو أسبوعين حتى اللون
+
+152
+00:10:33,950 --> 00:10:38,970
+يروح بعد غسيل مستمر بيجيب و ترك أثر وبالتالي لم
+
+153
+00:10:38,970 --> 00:10:42,670
+نستخدمه لا على الأسطح ولا على .. استخدمنا ليه؟
+
+154
+00:10:42,670 --> 00:10:45,530
+أغراض معينة زي مثلا كنا في الأوساط الزراعية
+
+155
+00:10:49,180 --> 00:10:51,320
+هذه اللغة مهمة كثير كثير كثير كثير كثير كثير كثير
+
+156
+00:10:51,320 --> 00:10:51,680
+كثير كثير كثير كثير كثير كثير كثير كثير كثير كثير
+
+157
+00:10:51,680 --> 00:10:56,700
+كثير كثير كثير كثير كثير كثير كثير كثير كثير كثير
+
+158
+00:10:56,700 --> 00:11:04,340
+كثير كثير كثير
+
+159
+00:11:04,340 --> 00:11:05,640
+كثير كثير كثير كثير كثير كثير كثير كثير كثير كثير
+
+160
+00:11:05,640 --> 00:11:07,980
+كثير كثير كثير كثير كثير كثير كثير كثير كثير كثير
+
+161
+00:11:07,980 --> 00:11:17,000
+كثير كثير كثير كثير كثير
+
+162
+00:11:17,000 --> 00:11:25,230
+كثيردرجات لغاية ما الان بدنا نطلع على نقطتين
+
+163
+00:11:25,230 --> 00:11:28,430
+مهمتين او مهمين في عملية ال killing لل micro
+
+164
+00:11:28,430 --> 00:11:34,910
+organism انه لما انا بدي افحص قدرة agent على قتل
+
+165
+00:11:34,910 --> 00:11:38,650
+البكتيريا بعدش البكتيريا اللي ماتت بعد البكتيريا
+
+166
+00:11:38,650 --> 00:11:42,810
+اللي بقيت حية هذا اللي بقدر اعمله انا انا بقدر اعد
+
+167
+00:11:42,810 --> 00:11:46,570
+البكتيريا الحية اما اللي ماتت مابقدرش اعدها فبكون
+
+168
+00:11:46,570 --> 00:11:51,630
+عارف العادل اصليولمّا البكتيريا الحيّة قعدّها،
+
+169
+00:11:51,630 --> 00:11:55,250
+بتروح وضّل منها في الميت، فإحنا غالبًا بنتعامل مع
+
+170
+00:11:55,250 --> 00:12:00,690
+البكتيريا التي بقيت حيّة، ال survivors، من
+
+171
+00:12:00,690 --> 00:12:05,590
+البكتيريا، بقولنا إن ال number of survivors ده
+
+172
+00:12:05,590 --> 00:12:10,810
+البكتيريا تتناسب تناسبا عكسيا مع C و T، إيش ال C و
+
+173
+00:12:10,810 --> 00:12:14,990
+T؟ ال C هو ال concentration of the killing agent،
+
+174
+00:12:14,990 --> 00:12:19,810
+أعتقد أنه بقولكوا قرأته على اللوحةأو على البطل تبع
+
+175
+00:12:19,810 --> 00:12:24,930
+الكحول اللي في المختبر 70% الكحول و كلمة 70% هي
+
+176
+00:12:24,930 --> 00:12:29,930
+concentration ليش ما بيقولنا 50% 80% مظبوط يبقى في
+
+177
+00:12:29,930 --> 00:12:35,230
+الآخر 70% معناها concentration the length of time
+
+178
+00:12:35,230 --> 00:12:40,610
+agent is applied انتوا بتعرفوا انه الزمن عامل مهم
+
+179
+00:12:40,610 --> 00:12:45,310
+في كل حاجة و أعطيكوا مثال بسيط لو ولعنا و لع أو
+
+180
+00:12:45,310 --> 00:12:50,690
+بلعك و لع الغازحطينا إيدينا على مسافة مثلا عشرين
+
+181
+00:12:50,690 --> 00:12:58,430
+صنطي هل بنشعر بلسحة النار فورا ولا بعد فترة معينة
+
+182
+00:12:58,430 --> 00:13:02,170
+نقدرش نتحمل الحرارة فالتأدير ايش كان عبارة عن
+
+183
+00:13:02,170 --> 00:13:06,810
+تراكمي مع الزمن تراكمي مع الزمن يبقى ال exposure
+
+184
+00:13:06,810 --> 00:13:12,010
+time أوالـ length of time ailent is applied is
+
+185
+00:13:12,010 --> 00:13:16,090
+very essential مهم جدا يبقى فيه two factors ايش هم
+
+186
+00:13:16,090 --> 00:13:20,590
+كم مرة؟ ال concentration و ال time بقولنا ان ال
+
+187
+00:13:20,590 --> 00:13:23,710
+number of survivors تلاتة و تلاتة سببين عكسيا مع
+
+188
+00:13:23,710 --> 00:13:27,630
+ال concentration و ال time يعني كلما زاد ال time
+
+189
+00:13:27,630 --> 00:13:31,630
+كلما زاد ال concentration كلما قل عدد اسم .. مش كل
+
+190
+00:13:31,630 --> 00:13:36,940
+.. يعني كلما تكون علاقة عكسية قلعكسيا ما بيحكي انه
+
+191
+00:13:36,940 --> 00:13:40,560
+كل ما زاد الزمن كل ما زاد ال concentration كل ما
+
+192
+00:13:40,560 --> 00:13:42,920
+قل على ال survival، يعني بقى ماناخة كل ما زاد
+
+193
+00:13:42,920 --> 00:13:46,720
+القتل، كل ما زاد القتل، الجالسة النقطة كتير معلومة
+
+194
+00:13:46,720 --> 00:13:51,780
+أو مفهومة وبيجب أن نستفيد منها، بنفعك نيجي نمسح
+
+195
+00:13:51,780 --> 00:13:55,400
+الأسطح اللي في المستشفيات عشان بس نقول والله
+
+196
+00:13:55,400 --> 00:14:00,100
+طهرنا، لازم نترك وقت زمنمعتمد على ال
+
+197
+00:14:00,100 --> 00:14:02,640
+concentration، يعني لو كانت ال concentration
+
+198
+00:14:02,640 --> 00:14:06,420
+خمسين، حسيبه زمن أطول من لو كانت ال concentration
+
+199
+00:14:06,420 --> 00:14:10,000
+سبعين، هدول العاملين متفاعلين مع بعض، ال
+
+200
+00:14:10,000 --> 00:14:13,480
+concentration هي الزمن طب خدوا بالك، احنا بعض
+
+201
+00:14:13,480 --> 00:14:17,460
+الأحيان هدول العاملين مع بعض، يعني بيجبرونا أن
+
+202
+00:14:17,460 --> 00:14:22,740
+نفكر بطرق متنوعة الكحول على سبيل المثال، تركيز
+
+203
+00:14:22,740 --> 00:14:26,390
+خمسين في المية بيقتل البكتيريالكن الزمن اللازم بده
+
+204
+00:14:26,390 --> 00:14:30,070
+تلت دقائق تقريبا، تلت دقائق، في حد فينا بيغسل إيدي
+
+205
+00:14:30,070 --> 00:14:34,270
+بالكحول، بيقعد فيهم تلت دقائق، كام دقيق، كام ثاني،
+
+206
+00:14:34,270 --> 00:14:37,430
+ثواني بنستخدم صح ولا ثواني، وانتهى الموضوع، ف
+
+207
+00:14:37,430 --> 00:14:40,450
+practically احنا بنرفع ال concentration عشان نقل
+
+208
+00:14:40,450 --> 00:14:44,210
+من الزمن في هذه الحالة، لكن خدوا بالك، ولي كل
+
+209
+00:14:44,210 --> 00:14:48,750
+قاعدة دايما فيها شوية، الكحول بنسبة مائة بالمائة
+
+210
+00:14:54,050 --> 00:14:59,230
+الجهول بنسبة مائة بالمائة غير فعال، بس بنسبة تسعين
+
+211
+00:14:59,230 --> 00:15:03,190
+بالمائة فعال، وتمانين بالمائة فعال، وسبعين، وستين،
+
+212
+00:15:03,190 --> 00:15:08,250
+وخمسين، ليش اخترنا سبعين بالذات؟لجينا إن الـ 70 at
+
+213
+00:15:08,250 --> 00:15:13,070
+one second أو two seconds كافر القتل طب و أنا بقدر
+
+214
+00:15:13,070 --> 00:15:16,170
+أمسح إيدي بأقل من one second أصلا بقدرش وبالتالي
+
+215
+00:15:16,170 --> 00:15:19,730
+مناسبة طب مش مختلف بستخدام اش تمانين تمانين أغلى
+
+216
+00:15:19,730 --> 00:15:24,790
+تمانين بتكلفني فلوس concentration أعلى مظبوط
+
+217
+00:15:24,790 --> 00:15:28,710
+بتكلفني مادة فعالة أعلى وبالتالي فيها فلوس أكتر
+
+218
+00:15:28,710 --> 00:15:31,970
+يبقى في أكتر من عامل بيخلنا نختار إيه ال rise
+
+219
+00:15:31,970 --> 00:15:35,920
+concentrationلو روحنا على المستشفى و قلنا لهم يا
+
+220
+00:15:35,920 --> 00:15:39,060
+جماعة إيش في عندكم مواد مطهرة بتستخدموها في
+
+221
+00:15:39,060 --> 00:15:44,800
+المستشفى؟ هيذكروننا مجموعة أسماء، هنسألهم ألا يوجد
+
+222
+00:15:44,800 --> 00:15:50,260
+لديكم نوع واحد بيغني عن كل هدول الأشياء، هيقولوننا
+
+223
+00:15:50,260 --> 00:15:55,280
+لأسف لأ، للأسف لأ، ذكرنا أحد الأسباب أنه ممكن يكون
+
+224
+00:15:55,280 --> 00:15:59,760
+.. أعطيكم مثال أنا من حياتي كل يومية، بدك تشتري
+
+225
+00:15:59,760 --> 00:16:05,010
+laptop، هبتقولي بدي مواصفاتبدي كارت شاشة مواصفاته
+
+226
+00:16:05,010 --> 00:16:08,510
+مش عارف اتنين جيجا، بدي hard disk واحد تيرة، بدي
+
+227
+00:16:08,510 --> 00:16:12,630
+ال memory تبعته مش عارف ال cash تبعه وكده، انهم
+
+228
+00:16:12,630 --> 00:16:15,790
+بروح السوق بنلاقي تلاتة من الاربع مواصفات اللي
+
+229
+00:16:15,790 --> 00:16:20,230
+طلبناهم قولين في laptop و التاني اتنين من اربع،
+
+230
+00:16:20,230 --> 00:16:26,990
+واحد من اربع كمان، لكن للأسف مالاجيناش laptop واحد
+
+231
+00:16:26,990 --> 00:16:31,170
+اللي فيه الأحلام تبعتنا، اللي بدنا هيها احنا في
+
+232
+00:16:31,170 --> 00:16:35,320
+المواصفاتفبنتضطر إنه إذا أنا بحتاج هذه المواصفات
+
+233
+00:16:35,320 --> 00:16:37,680
+إن أنا أشتري لابتوب هذا و أشتري لابتوب هذا و أشتري
+
+234
+00:16:37,680 --> 00:16:41,580
+لابتوب هذا إذا بستخدمه الأغراض متنوعة و مختلفة
+
+235
+00:16:41,580 --> 00:16:45,280
+وهذا اللي هي بيصير بالفعل في المختبرات ال ideal
+
+236
+00:16:45,280 --> 00:16:50,200
+antiseptic أو disinfectant موصل نهر يعني it must
+
+237
+00:16:50,200 --> 00:16:54,080
+be cheap لازم يكون رخيص it must be effective at
+
+238
+00:16:54,080 --> 00:16:58,960
+low concentration it must be safe مافيش irritation
+
+239
+00:16:58,960 --> 00:17:02,430
+مالهوش رائحةكريهة على الأقل بيروح المستشفى
+
+240
+00:17:02,430 --> 00:17:06,070
+بيلاقيهم ناطفيه الأرضيات بكلور ريحت المستشفى تكون
+
+241
+00:17:06,070 --> 00:17:09,490
+سيئة للغاية صح دلوقت و قد تؤذي المريض بعض الناس
+
+242
+00:17:09,490 --> 00:17:13,910
+هيتحسسوا من ال .. في مواد قاتلة جدا للبكتيريا
+
+243
+00:17:13,910 --> 00:17:18,750
+وسامة للإنسان فبالتالي المواصفات الميثالية كمان
+
+244
+00:17:18,750 --> 00:17:22,750
+مرة اللي احنا بيزنيها مش متوفرة في agent واحد لكن
+
+245
+00:17:22,750 --> 00:17:26,170
+ممكن نلاقي في هذا ال agent خمسة من عشرة ستة من
+
+246
+00:17:26,170 --> 00:17:29,230
+عشرة سبعة من عشرة و بالتالي بنستخدم الأجراض محددة
+
+247
+00:17:35,560 --> 00:17:39,040
+أحنا قلنا راح نقسم العوامل ل chemical and physical
+
+248
+00:17:39,040 --> 00:17:47,060
+agents بس في نقطة كتير قبل نقمز عنها في اللي لو
+
+249
+00:17:47,060 --> 00:17:53,580
+حصلها لقها احنا التعريف الطبي للموت عند البشر توقف
+
+250
+00:17:53,580 --> 00:17:55,200
+القلب او توقف الدماء
+
+251
+00:17:59,210 --> 00:18:05,750
+بيعد إلى الموت؟ بس بس بس بس بس بس بس بس بس بس بس
+
+252
+00:18:05,750 --> 00:18:10,870
+بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس
+
+253
+00:18:10,870 --> 00:18:13,590
+بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس
+
+254
+00:18:13,590 --> 00:18:13,790
+بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس
+
+255
+00:18:13,790 --> 00:18:14,070
+بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس
+
+256
+00:18:14,070 --> 00:18:14,230
+بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس
+
+257
+00:18:14,230 --> 00:18:20,190
+بس بس بس بس بس بس بس بس بس بس بس بس بس
+
+258
+00:18:20,790 --> 00:18:24,450
+Death is defined by the inability to reproduce
+
+259
+00:18:24,450 --> 00:18:29,390
+مدام مش قادر الميكروبيت كاتر فهو ميت هذا لغرض
+
+260
+00:18:29,390 --> 00:18:36,290
+تعريف ال disinfection طيب في نقطة كتير مهمة الان
+
+261
+00:18:36,290 --> 00:18:40,970
+لو أجى واحد و جالنا عرض علينا antiseptic جديد او
+
+262
+00:18:40,970 --> 00:18:45,290
+disinfectant جديد ال disinfectant هذا بدنا نقارنه
+
+263
+00:18:45,290 --> 00:18:50,100
+بدنا نشوف فعاليته هل هو فعال او مش فعالبنفعش دايما
+
+264
+00:18:50,100 --> 00:18:55,440
+كلمة انقارن بدها اتنين، بدها اتنين، على الأقل بدنا
+
+265
+00:18:55,440 --> 00:18:59,460
+واحد معيار و بدنا واحد انقارنه في المعيار، صح؟
+
+266
+00:18:59,460 --> 00:19:03,860
+المعيار اللي مورود عندنا اسمه فينول الفينول أحد
+
+267
+00:19:03,860 --> 00:19:10,300
+المركبات الأولى
+
+268
+00:19:10,300 --> 00:19:13,140
+اللي استخدمت في عمليات التطهير و disinfection
+
+269
+00:19:13,140 --> 00:19:18,540
+وبالتالي أي مركب جديد بنقارنه مين؟ بالفينولتعالى
+
+270
+00:19:18,540 --> 00:19:22,960
+بس لاني بلغة بسيطة عشان مش مجان نشرح بالضبط تفاصيل
+
+271
+00:19:22,960 --> 00:19:27,900
+كيفية العملية بالتم لكن نشرح الفكرة الان الفينول
+
+272
+00:19:27,900 --> 00:19:35,740
+عند عين تركيز X بيقتل و زمان Y بيقتل عشرة أس ستة
+
+273
+00:19:35,740 --> 00:19:39,980
+من البكتيريا تمام؟ المادة الجديدة اللى بدي إياها
+
+274
+00:19:39,980 --> 00:19:46,240
+أنا المادة الجديدة اللى بدي إياها بدي أعرف at what
+
+275
+00:19:46,240 --> 00:19:55,190
+concentrationat what time؟ at the same time at the
+
+276
+00:19:55,190 --> 00:19:59,710
+same time الناس راح تبقى هنا و الكل عشرة او ستة
+
+277
+00:19:59,710 --> 00:20:06,490
+ايش اللي fixed هنا؟ الزمن؟ الزمن صح؟ و عادل
+
+278
+00:20:06,490 --> 00:20:09,630
+البكتيريا؟ مين ضلحت هنا المضايقات؟ اللي هو ال
+
+279
+00:20:09,630 --> 00:20:13,310
+concentration ال phenol coefficient هو عبارة عن ال
+
+280
+00:20:13,310 --> 00:20:18,950
+concentration of phenolعلى ال concentration of ال
+
+281
+00:20:18,950 --> 00:20:25,310
+agent X العمل الجديد هذا تخيلوا
+
+282
+00:20:25,310 --> 00:20:30,730
+لو ال concentration طلعت 20 و أنا عشرين تمام؟ ايش
+
+283
+00:20:30,730 --> 00:20:35,070
+بتقول ال coefficient؟ واحد ايش معنى الحكاية؟ انه
+
+284
+00:20:35,070 --> 00:20:41,930
+العامل هذا الجديد اللي هو X is comparable نقارنه
+
+285
+00:20:41,930 --> 00:20:45,450
+بمين؟ بالفنون يعني نفس الكفاءة مع مين؟ مع الفنون
+
+286
+00:20:46,910 --> 00:20:55,090
+point واحد ايه ال coefficient؟ اقل او اكتر؟ احسن
+
+287
+00:20:55,090 --> 00:21:03,110
+او اقل؟ احسن او اقل؟ احسن او اقل؟ احسن او اقل؟
+
+288
+00:21:03,110 --> 00:21:04,830
+احسن او اقل؟ احسن او اقل؟ احسن او اقل؟ احسن او
+
+289
+00:21:04,830 --> 00:21:05,850
+اقل؟ احسن او اقل؟ احسن او اقل؟ احسن او اقل؟ احسن
+
+290
+00:21:05,850 --> 00:21:09,310
+او اقل؟ احسن او اقل؟ احسن او اقل؟ احسن او اقل؟
+
+291
+00:21:09,310 --> 00:21:09,350
+احسن او اقل؟ احسن او اقل؟ احسن او اقل؟ احسن او
+
+292
+00:21:09,350 --> 00:21:18,090
+اقل؟ احسن او اقل؟ احسن االعمل الجديد، لما نقول
+
+293
+00:21:18,090 --> 00:21:22,870
+تركيز أعلى، يعني كفاءة أقل، يبقى لما يكون ال point
+
+294
+00:21:22,870 --> 00:21:25,630
+.. لما يكون ال coefficient أقل من واحد، يكون
+
+295
+00:21:25,630 --> 00:21:29,810
+العامل أقل من كفاءة main الفنوة، إذا كان أكثر من
+
+296
+00:21:29,810 --> 00:21:34,230
+واحد العامل تمام، مانا الحاجة إنه الكفاءة الصالح
+
+297
+00:21:34,230 --> 00:21:35,850
+للعمل ال major
+
+298
+00:21:45,110 --> 00:21:50,590
+مهم جدا لأنه بيجينا كتير مراكبات باستمرار احنا
+
+299
+00:21:50,590 --> 00:21:56,190
+لازم نحدد فعاليتها عن طريق مقارنة بمين بالفينول
+
+300
+00:21:56,190 --> 00:22:00,430
+ويسمى فينول coefficient معامل الفينول
+
+301
+00:22:09,870 --> 00:22:12,610
+بقولنا الـ quantitative measure of this variation
+
+302
+00:22:12,610 --> 00:22:15,750
+is expressed as the phenol coefficient الكلام اللي
+
+303
+00:22:15,750 --> 00:22:18,110
+حكيناه جاب شويه الـ phenol coefficient اللي هو
+
+304
+00:22:18,110 --> 00:22:24,330
+معامل الـ phenol لماذا إلجأنا لفحص أو لعمل أو
+
+305
+00:22:24,330 --> 00:22:28,930
+لتحديد الـ phenol coefficient بغرض المقارنة عشان
+
+306
+00:22:28,930 --> 00:22:32,410
+نحدد ال variation هحدد اللي هو ال variation مهم
+
+307
+00:22:32,410 --> 00:22:35,110
+جدا نحدد ال variation عشان نحدد ال concentration
+
+308
+00:22:35,110 --> 00:22:40,190
+اللي بدي أستخدمها و ال time اللي أنا حاملهExposure
+
+309
+00:22:40,190 --> 00:22:46,290
+للأسطح اللي أنا بدي طهرها أو بدي أعقب هناك ثلاثة
+
+310
+00:22:46,290 --> 00:22:51,650
+آليات مشهورة جدا في ال disinfectant و ال
+
+311
+00:22:51,650 --> 00:22:55,130
+antiseptics wherein the agent may kill the
+
+312
+00:22:55,130 --> 00:22:58,330
+microorganism الأولى by cell membrane damage
+
+313
+00:22:58,330 --> 00:23:02,130
+التانية by protein denaturation تلاتة by DNA
+
+314
+00:23:02,130 --> 00:23:05,690
+damage أو modification يعني واحدة من تلاتة يا إما
+
+315
+00:23:05,690 --> 00:23:08,230
+على ال cell membraneأو على الـ membrane أو على الـ
+
+316
+00:23:08,230 --> 00:23:12,050
+phospholipid يما على البروتيني انه .. لكن بدكوا
+
+317
+00:23:12,050 --> 00:23:16,210
+تلاحظوا نقطة كتير مهمة في هذا الجانب مختلفة عن ال
+
+318
+00:23:16,210 --> 00:23:20,050
+antibiotics ال antibiotics .. ال antibiotics
+
+319
+00:23:20,050 --> 00:23:26,370
+بتشتغل على خطوة واحدة very very specificالانتيبايت
+
+320
+00:23:26,370 --> 00:23:30,790
+بيشتغل عليها very specific step يعني مثلا ابترتبط
+
+321
+00:23:30,790 --> 00:23:35,810
+بال 50S-ribosomal subunit وبتمنع ال translocation
+
+322
+00:23:35,810 --> 00:23:39,670
+لل amino acids الجديدة وبالتالي بتوقف تصنيع ال
+
+323
+00:23:39,670 --> 00:23:43,270
+protein بينما هذا اللي هتقولك modification of
+
+324
+00:23:43,270 --> 00:23:49,750
+proteins اي protein بيخربه اي DNA بيخربه وبالتالي
+
+325
+00:23:49,750 --> 00:23:53,610
+بدنا نفهم ان الميكانيزم هنا مش كتير specific
+
+326
+00:23:54,410 --> 00:23:58,470
+بالإضافة إلى أن بعض المُركّبات قد تستطيع قتل
+
+327
+00:23:58,470 --> 00:24:03,790
+البكتيريا من خلال أكثر من آلية معينة وهذا مصطلح مش
+
+328
+00:24:03,790 --> 00:24:09,670
+مذكور معانا الآن، بدنا نكتبه اسمه الـ Oligodynamic
+
+329
+00:24:09,670 --> 00:24:15,590
+Action قدرنا
+
+330
+00:24:15,590 --> 00:24:20,930
+نكون فهم، يعني الـ agent هو واحد قادر على قتل
+
+331
+00:24:21,490 --> 00:24:25,550
+البكتيريا بأكثر من وسيلة مثلا بيعمل disruption للـ
+
+332
+00:24:25,550 --> 00:24:27,810
+lipid membrane وبنفس الوقت بيعمل protein
+
+333
+00:24:27,810 --> 00:24:33,630
+denaturation إيش سمينا هذا العامل؟ الريباندركتشون
+
+334
+00:24:33,630 --> 00:24:37,610
+ليش أنا حابب اتأكد على هذه المعلومة و أذكركم إن ال
+
+335
+00:24:37,610 --> 00:24:42,450
+antibiotic specific البكتيريا استطاعت بنجاح إنها
+
+336
+00:24:42,450 --> 00:24:46,490
+تكون resistance لكثير من ال antibiotic ليش؟ لأن ال
+
+337
+00:24:46,490 --> 00:24:50,750
+antibiotic بيأثر على one stepفلو صار ال mutation
+
+338
+00:24:50,750 --> 00:24:53,850
+فاكرين ال mutations المحاضرة السابقة؟ لو صار ال
+
+339
+00:24:53,850 --> 00:24:57,210
+mutation أو it acquired a gene of resistance
+
+340
+00:24:57,210 --> 00:25:00,730
+البكتيريا صارت resistant لمين؟ لل antibiotic لكن
+
+341
+00:25:00,730 --> 00:25:05,070
+لحد الآن كده استخدامنا ال detol تمام ال resistant
+
+342
+00:25:05,070 --> 00:25:08,550
+لل detol يكاد لا يذكر ال resistant لل chlorine
+
+343
+00:25:08,550 --> 00:25:12,670
+اللي هو ال chlorine يكاد لا يذكر لأنه جالبا هو ال
+
+344
+00:25:12,670 --> 00:25:16,010
+mechanism هي non specific و في نفس الوقت قد تكون
+
+345
+00:25:16,010 --> 00:25:17,770
+ايه؟ Oligodynamic
+
+346
+00:25:21,210 --> 00:25:23,690
+من المُركّبات اللي بتعمل disruption لـ cell
+
+347
+00:25:23,690 --> 00:25:29,730
+membrane الكحول المشهور بالـ Ethanol وفي اسم تاني
+
+348
+00:25:29,730 --> 00:25:32,550
+اللي لسه مش متقوله ان احنا هتذكره كمان شوية اللي
+
+349
+00:25:32,550 --> 00:25:37,930
+هو الـ isopropanol وفيه احنا الـ detergent هو
+
+350
+00:25:37,930 --> 00:25:42,770
+متنميز ال detergent ليه المنظفات وقد يتواضر للذهن
+
+351
+00:25:42,770 --> 00:25:46,110
+بعض الناس اللي بيقولون منظفات انه يخطر على باله
+
+352
+00:25:46,110 --> 00:25:51,040
+الصابون لأ الصابون ليس له علاقة بالمنظفاتو بتسمعوا
+
+353
+00:25:51,040 --> 00:25:54,880
+انتوا بعض اللي أحيان في الدعايات صابون متحر صابون
+
+354
+00:25:54,880 --> 00:25:59,720
+Detol على سبيل المثال و يقتل 99.9% من الجراثيم
+
+355
+00:25:59,720 --> 00:26:05,860
+طبعا هو بيقتل 100% بس ال 99.9% هذي بيحطوها كمرة
+
+356
+00:26:05,860 --> 00:26:09,160
+احنا في ال micro biology غالبا لا نتداعى الدقة
+
+357
+00:26:09,160 --> 00:26:14,400
+لإنه طريقة تفكيرنا مبنية على assumptions على
+
+358
+00:26:14,400 --> 00:26:18,250
+افتراضات ده بيجعلوا يشوفوا ان الموتيعرف بإنه عدم
+
+359
+00:26:18,250 --> 00:26:21,110
+القدرة على البكتيريا لتعمل reproduction لكن لو أنا
+
+360
+00:26:21,110 --> 00:26:25,050
+أخدت البكتيريا التعبانة اللي هي غير قادرة على ال
+
+361
+00:26:25,050 --> 00:26:28,770
+reproduction و حطيتها في enrichment media قد تعود
+
+362
+00:26:28,770 --> 00:26:33,350
+يعني قد نحييها مرة أخرى بالتعريف اللي هو الخاطأ
+
+363
+00:26:33,350 --> 00:26:36,130
+اللي موجود في ال assumption مش دقيقة وبالتالي
+
+364
+00:26:36,130 --> 00:26:40,690
+بنحاول قدر الإمكان إنه ما نعطي أيضا كمان أرقام
+
+365
+00:26:40,690 --> 00:26:45,700
+دقيقة لما قلنا لكوا PFUblack forming unit مابنهاش
+
+366
+00:26:45,700 --> 00:26:49,700
+بكتيريوفاج و لما نتعدى البكتيريا بنقول cfu colony
+
+367
+00:26:49,700 --> 00:26:53,300
+forming unit عشان برضه مانفترضش أننا بنعدى بكتيريا
+
+368
+00:26:53,300 --> 00:26:58,880
+و أننا بنعدى وحدة بناء مستمرة ال detergent غير عن
+
+369
+00:26:58,880 --> 00:27:05,060
+الصابون الصابون العادى اللى احنا بنقولكه اغسل يديك
+
+370
+00:27:05,060 --> 00:27:09,260
+بالماء والصابون باستمرار و نصيحة و موجودة في
+
+371
+00:27:09,260 --> 00:27:14,550
+المنزلوموجودة حتى في أكتر الأماكن يعني احتياجة
+
+372
+00:27:14,550 --> 00:27:17,970
+للتطهير والتعقيم، اللي هي المستشفيات أو العبادة
+
+373
+00:27:17,970 --> 00:27:23,690
+الجراحية، إذن هو ليس متهر الصابون وإنما .. وإنما
+
+374
+00:27:23,690 --> 00:27:27,410
+.. لأ مش منظف كمان، خلّيني أشرح هو، لأن كلمة منظف
+
+375
+00:27:27,410 --> 00:27:31,870
+هي مصطلح يطلق على مين؟ على الـ detergent، هي تقوم
+
+376
+00:27:31,870 --> 00:27:34,770
+بـmechanical removal of the microorganism، مش أنه
+
+377
+00:27:34,770 --> 00:27:38,250
+mechanicalيعني بتساعد على أنه البكتيرى اللى على
+
+378
+00:27:38,250 --> 00:27:43,750
+إيدينا تنزل تروح تمام بس مابيقتلهاش الصوان بينما
+
+379
+00:27:43,750 --> 00:27:47,650
+ال detergent ممكن يساعد في إزالتها وبيقتلها في نفس
+
+380
+00:27:47,650 --> 00:27:50,450
+الوجه، فب .. هذا مختلف يعني ال detergent غير عن
+
+381
+00:27:50,450 --> 00:27:53,170
+الصوان بالإضافة إلى ال phenols اللى أحنا حاكينا
+
+382
+00:27:53,170 --> 00:27:57,790
+عنهم إنه من أقدم المركبات المستخدمة في التطبيق نجل
+
+383
+00:27:57,790 --> 00:28:02,950
+ال EthanolEthanol is widely used to clean the skin
+
+384
+00:28:02,950 --> 00:28:07,270
+before immunization or venipuncture طبعا بتعرفين
+
+385
+00:28:07,270 --> 00:28:10,510
+اللي لما بناخدنا الطعام بمسحوناه في جطنه و باخدونا
+
+386
+00:28:10,510 --> 00:28:15,150
+القبره او لما بنسحب عين الدم بس الان بنتكلم على
+
+387
+00:28:15,150 --> 00:28:18,970
+سحب عين الدم في ال micro biology بتختلف عن سحب عين
+
+388
+00:28:18,970 --> 00:28:21,470
+الدم في ال hematology او ال chemical chemistry
+
+389
+00:28:21,470 --> 00:28:29,890
+الكحول ال 70% متحر والتطهير ليسabsolute killing
+
+390
+00:28:29,890 --> 00:28:34,490
+صح؟ اللي هو مش ده sterilization أنا بدي أحاول أعمل
+
+391
+00:28:34,490 --> 00:28:38,610
+ما يشبه ال sterilization في ال skin لما بدي أعمل
+
+392
+00:28:38,610 --> 00:28:41,530
+blood culture عارفين ليش؟ لإن بالضبط من إن ال
+
+393
+00:28:41,530 --> 00:28:45,190
+bacteria نمت معي في المختبر نمت معي من الدم و ليس
+
+394
+00:28:45,190 --> 00:28:49,330
+من مين؟ من الجلد لإن الجلد لما احنا دخلنا إلى الدم
+
+395
+00:28:49,330 --> 00:28:54,130
+دخلنا و أخدنا دم لكن عبر مين؟ الجلد فإذا كان في
+
+396
+00:28:54,130 --> 00:28:58,710
+بكتيريا على الجلد هترجع معاها هذا واحدوخدوا بألكوا
+
+397
+00:28:58,710 --> 00:29:02,650
+الأصل إن العملية هذه تتم حتى في سحب الدم بطريقة
+
+398
+00:29:02,650 --> 00:29:08,710
+روتينية لإنه ممكن ندخل أصلا بكتيريا إلى الدم، الآن
+
+399
+00:29:08,710 --> 00:29:14,570
+عشان نلجأ لهذه العملية، بنضطر نعمل عملية إضافية أو
+
+400
+00:29:14,570 --> 00:29:19,430
+عملتين إضافيتين، كحول نمسح فيه وعارفين الطريقة من
+
+401
+00:29:19,430 --> 00:29:24,430
+ال center لل outward طريقة الأولابية من المركز إلى
+
+402
+00:29:24,430 --> 00:29:28,880
+اللي هو قلنا إيه إلى الخارجمش من فوق لتحت أو من
+
+403
+00:29:28,880 --> 00:29:34,140
+تحت لفوق يعني إذا كان في أوساخ أوساخ موجودة ننزلها
+
+404
+00:29:34,140 --> 00:29:38,400
+تحت و بعدين نرفعها فوق و كأنك ما عملتيشي كأنك
+
+405
+00:29:38,400 --> 00:29:42,420
+ماعملتيشي هيجب أن تكون بشكل دائري لو اللابي بهذا
+
+406
+00:29:42,420 --> 00:29:48,520
+الشكل هذا رقم واحد رقم اتنين بنضيف .. بنضيف اللي
+
+407
+00:29:48,520 --> 00:29:55,220
+هو ال أيودين صبغة اليود و بنتركها على اليد لمدة
+
+408
+00:29:55,220 --> 00:30:02,130
+دقيقتينوبعد ما يخلصوا الدقيقتين بنروح واحنا شايلين
+
+409
+00:30:02,130 --> 00:30:07,650
+الأيودين بقطنة محتوية على 70% الكحول، يبقى كحول،
+
+410
+00:30:07,650 --> 00:30:12,530
+أيودين، وبعدين كحول، هذا بيضمن لإن الأيودين عنده
+
+411
+00:30:12,530 --> 00:30:18,000
+قدرة قتل أكبر بكثير من مين من الكحولبيضمنلي انه
+
+412
+00:30:18,000 --> 00:30:21,680
+يعني بنسبة تسعة وتسعين بالمئة نجمي مول اكتر من ذلك
+
+413
+00:30:21,680 --> 00:30:24,580
+انه مافيش ضايق لإيه مايكرو أورجينزم لإن الأيودين
+
+414
+00:30:24,580 --> 00:30:29,340
+مشهور عنه قدرته على انه بسموه ده germicidal دلوقت
+
+415
+00:30:29,340 --> 00:30:32,900
+انه بيقتل البكتيريا و الفطريات و الفيروسات حتى ال
+
+416
+00:30:32,900 --> 00:30:37,540
+sports فهو فريد من مين؟ من إنه يكون sterilant مادة
+
+417
+00:30:37,540 --> 00:30:43,880
+معاقبةأعتقد إن إحنا حكينا عن ال 70% و عن ال 100% و
+
+418
+00:30:43,880 --> 00:30:48,420
+كنا إن ال 100% يرفع أعلى لسبب
+
+419
+00:30:50,870 --> 00:30:53,210
+لأ انا بالدولة عاوزة تفضل اللي بتديله مش هو انا
+
+420
+00:30:53,210 --> 00:30:57,950
+لما زيد الزمن زيدي التركيز بيزيد الزمن؟ لأ عكس هذا
+
+421
+00:30:57,950 --> 00:31:01,590
+الاستثناء و هذا الاستثناء بالعكس لما يزيد التركيز
+
+422
+00:31:01,590 --> 00:31:07,290
+بيقل الزمن طب كيف انه هي العلاقة أن تتناسب ترضي مع
+
+423
+00:31:07,290 --> 00:31:10,730
+سيء في team مش ترضي مش ترضي عكس طب ماجي لكن
+
+424
+00:31:10,730 --> 00:31:16,090
+التركيز و الزمن ترضي بين بعض عشانالتركيز و الزمن
+
+425
+00:31:16,090 --> 00:31:19,390
+.. و الزمن .. طرد .. مش قولنا كل ما زادت التركيز
+
+426
+00:31:19,390 --> 00:31:22,470
+.. لأ يا بنتي لأ لأ لأ .. كل ما زادت التركيز قل
+
+427
+00:31:22,470 --> 00:31:26,250
+الزمن .. قل الزمن، هذا علاقة غير علاقة الأولانية،
+
+428
+00:31:26,250 --> 00:31:28,870
+علاقة الأولانية كانت number of survivors، فتناسى
+
+429
+00:31:28,870 --> 00:31:33,770
+الترسم عكسيا مع العاملين مع بعض، مضلوبين في بعض،
+
+430
+00:31:33,770 --> 00:31:36,770
+التناسي مع عكس طبعا .. the more .. the more .. the
+
+431
+00:31:36,770 --> 00:31:39,270
+higher the concentration, the less the time is
+
+432
+00:31:39,270 --> 00:31:44,290
+required، يعني علق عكسيالكن هذه الاستثناء قلنا انه
+
+433
+00:31:44,290 --> 00:31:48,830
+ال concentration لحد معين كل ما ارتفعنا كل ما زادت
+
+434
+00:31:48,830 --> 00:31:52,130
+الكفاءة كل ما زادت الكفاءة لكن لكن هذا ان المائة
+
+435
+00:31:52,130 --> 00:31:57,470
+بالمائة من المركب كفائتها منخفضة ايش السبب؟ ان
+
+436
+00:31:57,470 --> 00:32:01,430
+الكهوية كان يعمل محتاج ماء مائة بالمائة كهوية
+
+437
+00:32:01,430 --> 00:32:06,710
+مافيش ميه قدرته على ال penetration بتكون اقل وإذا
+
+438
+00:32:06,710 --> 00:32:10,470
+ما اخترقش داخل الخلية مانحك مش هعمل destruction
+
+439
+00:32:10,470 --> 00:32:14,250
+للمنبرينولا ال proteins هو بيشتغل بالطريقتين طريقة
+
+440
+00:32:14,250 --> 00:32:18,070
+انه بيعمل disorganization ل ال membrane lipid و
+
+441
+00:32:18,070 --> 00:32:21,550
+بيعمل denaturation لمن؟ ل ال proteins إذا هو من
+
+442
+00:32:21,550 --> 00:32:25,510
+العوامل اللي بتشتغل oligodynamic action لذلك أنا
+
+443
+00:32:25,510 --> 00:32:30,110
+لحد الآن لم أقرا بكتيريا مقاومة طبعا يعني كانت
+
+444
+00:32:30,110 --> 00:32:34,490
+sensitive قصارة مقاومة أنا مبسوط فيها للكحول لإن
+
+445
+00:32:34,490 --> 00:32:38,170
+الكحول جدنا بيقضي عليها بطوله non specific لكن
+
+446
+00:32:38,170 --> 00:32:43,540
+تخيلوا لو بيشتغل على one step فقطفيه chance .. فيه
+
+447
+00:32:43,540 --> 00:32:45,760
+chance عالية أن البكتيريا تكون له resistance
+
+448
+00:32:45,760 --> 00:32:49,680
+بتعطيكوا مثال بس يعني خارج الإطار عشان برضه الصورة
+
+449
+00:32:49,680 --> 00:32:55,340
+تُضح بشكل جيد هناك حالة بنتسميها Combined Therapy
+
+450
+00:32:55,340 --> 00:32:59,540
+علاج مشترك بمعنى أنه مانعطيش Antibiotic، مانعطي 2
+
+451
+00:32:59,540 --> 00:33:02,160
+أو 3 أو 4 Antibiotics يعني في مثلا الـ
+
+452
+00:33:02,160 --> 00:33:05,160
+Tuberculosis، مانعطيله أربع أنواع من المضاد
+
+453
+00:33:05,160 --> 00:33:11,500
+الحيوية تعني نسميهم ألف A وB وC وD وهي البكتيريا
+
+454
+00:33:12,500 --> 00:33:18,920
+اللي هي Mycobacterium Tuberculosis الآن لو كوّنت
+
+455
+00:33:18,920 --> 00:33:29,880
+resistant لإيه؟ B و C و D يقتلوها، صح؟ يبقى
+
+456
+00:33:29,880 --> 00:33:34,480
+البكتيريا اللي كوّنت resistant لإيه؟ ظهرت على
+
+457
+00:33:34,480 --> 00:33:38,740
+العلم؟ ولا ماتت في مهدها؟ قُتلت في مهدها، لم تنشأ
+
+458
+00:33:38,740 --> 00:33:43,770
+منها سلولات لأنه قتلت .. احنا قطيهم مع بعضطيب لو
+
+459
+00:33:43,770 --> 00:33:52,690
+جللنا ال antibiotic وصحيح ان a,b,c بالان كومة
+
+460
+00:33:52,690 --> 00:33:58,610
+resistant ل a قتلها مين؟ b و c لكن احتمالية ان
+
+461
+00:33:58,610 --> 00:34:05,370
+البكتيريا تكون resistant ل b و c في وجود واحد رابع
+
+462
+00:34:05,370 --> 00:34:11,370
+او خامس او سادس بتكون عايشة اقل فكل ما زال عد ال
+
+463
+00:34:11,370 --> 00:34:14,830
+antibioticsاللي انا بدي افترض ان كل انتيباتك له
+
+464
+00:34:14,830 --> 00:34:19,450
+ميكانيزم of action فلما يكون ال disinfectant بيقتل
+
+465
+00:34:19,450 --> 00:34:23,290
+البكتيريا ب different mechanisms لما لو قورت
+
+466
+00:34:23,290 --> 00:34:26,890
+resistant لميكانيزم هيقتلها بميكانيزم أخرى
+
+467
+00:34:26,890 --> 00:34:30,990
+وبالتالي لن تظهر إلى العالم و تبقى في السوق يعني
+
+468
+00:34:30,990 --> 00:34:35,830
+في الدنيا و بتموت في مهدها و تلصورها فكويس جدا
+
+469
+00:34:35,830 --> 00:34:38,030
+يكون عندي حاسب ما اقول لكوا dynamic action
+
+470
+00:34:42,270 --> 00:34:46,730
+من طبعا الكحولات المشهورة جدا في العالم بحاجة
+
+471
+00:34:46,730 --> 00:34:50,530
+اسمها isopropanoid بذكر الأسفلات اللي كتبوها ايزو
+
+472
+00:34:50,530 --> 00:34:54,490
+برضه
+
+473
+00:34:54,490 --> 00:34:59,030
+بيستخدموه بتركيز 70% من ميزاته انه بيحافظ على
+
+474
+00:34:59,030 --> 00:35:04,550
+رطوبة الجلد اكثر من ال Ethanol خدوا بالكم النقطة
+
+475
+00:35:04,550 --> 00:35:10,310
+هانفي التعامل في المستشفيات احنا بننصح انه بين كل
+
+476
+00:35:10,310 --> 00:35:16,350
+مريض و مريض مثلا الطبيب انه يطهر ايديه تطهير
+
+477
+00:35:16,350 --> 00:35:19,750
+الأيدان بين .. لو بشوفله عشرين تلاتين مريض يعني
+
+478
+00:35:19,750 --> 00:35:24,150
+مادي حاجة هيغسل ايديه عشرين مرة ما بيخلص اخر نهار
+
+479
+00:35:24,150 --> 00:35:28,650
+اللي هو جلده ناشف متشقق بصير هو عرض اصلا للاتهابات
+
+480
+00:35:28,650 --> 00:35:33,710
+الشخص هذا للطبيب او ال nurse وبالتالي تطهير
+
+481
+00:35:33,710 --> 00:35:38,340
+والتعقيم أنتج مشكلة جديدةفاضطرينا نحلها بإنه
+
+482
+00:35:38,340 --> 00:35:45,260
+نستخدم مراكبات أقل حدة على الجل مثلا لازربربنال
+
+483
+00:35:45,260 --> 00:35:50,100
+واحد منهم أو في هسمة alcohol gel ال alcohol gel
+
+484
+00:35:50,100 --> 00:35:55,840
+بيستخدموه متعالي اللي بيكون عبارة عن جل و نحطه على
+
+485
+00:35:55,840 --> 00:36:02,360
+جل و بعدين هذا بيقلل من اللي هو فقدان الرطوبة في
+
+486
+00:36:02,360 --> 00:36:07,770
+الإيدين وبالتالي بيحافظ على الإيدينرطبة وليس جياب
+
+487
+00:36:07,770 --> 00:36:12,950
+رقم
+
+488
+00:36:12,950 --> 00:36:16,030
+اتنين detergent رقم اتنين هو ال detergent و ال
+
+489
+00:36:16,030 --> 00:36:19,270
+detergent كما وصفته انا و ليس الصابون و انما هو
+
+490
+00:36:19,270 --> 00:36:25,070
+يبقى surface active agent عامل له نشاط سطحي و
+
+491
+00:36:25,070 --> 00:36:27,590
+بيسموه surfactant اللي هو المصطلح اللي هنا
+
+492
+00:36:27,590 --> 00:36:33,610
+surfactant تمام؟ بيمتلك hydrophilicو hydrophobic
+
+493
+00:36:33,610 --> 00:36:38,710
+ends مين من المركبات اللي درسناها في البكتيريا
+
+494
+00:36:38,710 --> 00:36:42,150
+عندها hydrophilic و hydrophobic end؟ ال cell
+
+495
+00:36:42,150 --> 00:36:45,890
+membrane و ال outer membrane تبع ال gram negative
+
+496
+00:36:45,890 --> 00:36:52,350
+بكتيريا، مضطر لأ؟ طيب لما ييجي surfactant إله
+
+497
+00:36:52,350 --> 00:36:57,770
+hydrophobic end و يتفاعل مع ال hydrophilic end تبع
+
+498
+00:36:57,770 --> 00:37:02,460
+ال cell membraneمين أقوى ال surfactant ولا ال cell
+
+499
+00:37:02,460 --> 00:37:09,100
+membrane فبروح اللي هو شادد ممزق ال cell membrane
+
+500
+00:37:09,100 --> 00:37:12,480
+فاكرين ال cell membrane كيف وصفناها لما وصفنا زيت
+
+501
+00:37:12,480 --> 00:37:20,200
+زيتون يعني لزج جدا مرن جدا رقيق جدا طبعا صلاته
+
+502
+00:37:20,200 --> 00:37:25,320
+ضعيفة جدا وبالتالي من السهل تمزيقه لكن بتلاعب ان
+
+503
+00:37:25,320 --> 00:37:31,470
+ال surfactant بيشتغلو احنا مش قولنا بدمر الcell
+
+504
+00:37:31,470 --> 00:37:36,210
+membrane عبر مين؟ عبر ال hydrophobic chain و
+
+505
+00:37:36,210 --> 00:37:40,570
+بيرتبط مع الميه في المحيط عبر ال hydrophobic chain
+
+506
+00:37:40,570 --> 00:37:44,590
+فبيقتل البكتيريا و ممكن يزيلها بمعنى اخر زي ما
+
+507
+00:37:44,590 --> 00:37:48,070
+بيشتغل مع الأوساخ، بيرتبط فيها و بيسحبها
+
+508
+00:37:50,720 --> 00:37:54,380
+من أشهر المركبات المشهورة حاجة اسمها Benzyl Konium
+
+509
+00:37:54,380 --> 00:37:59,000
+Chloride وهو يستخدم ك mild .. mild disinfectant أو
+
+510
+00:37:59,000 --> 00:38:02,420
+skin disinfectant وهو يتبع إلى ما يسمى بال
+
+511
+00:38:02,420 --> 00:38:06,320
+Quaternary Ammonium Sources Quaternary Ammonium
+
+512
+00:38:06,320 --> 00:38:10,260
+Compounds أو Quaternary Ammonium Source Benzyl
+
+513
+00:38:10,260 --> 00:38:15,760
+Konium استخدمت كايش ك skin العملية اللي بيسميها
+
+514
+00:38:15,760 --> 00:38:17,360
+disinfection
+
+515
+00:38:19,840 --> 00:38:22,640
+المُركّب التالف في السلسلة اللي هو الفينول
+
+516
+00:38:22,640 --> 00:38:26,560
+والفينول زي ما حكيتها لك هو ال first disinfectant
+
+517
+00:38:26,560 --> 00:38:30,620
+used in the operating room by Joseph Lister في ال
+
+518
+00:38:30,620 --> 00:38:36,720
+1860 أو ستينات القرن التاسع عشر تمانيات القرن ..
+
+519
+00:38:36,720 --> 00:38:41,320
+عفوا تمانيات القرن التاسع عشر Joseph Lister أُعطى
+
+520
+00:38:41,320 --> 00:38:45,080
+ال credit كله أُعطى يعني ال .. ال .. انه .. ان هو
+
+521
+00:38:45,080 --> 00:38:50,090
+صاحب التعقيم والتطهير في .. في المستشفياتلكن واقع
+
+522
+00:38:50,090 --> 00:38:54,650
+الانور انا لازم احكيلكوا قصة صغيرة عن احد الأطباء
+
+523
+00:38:54,650 --> 00:38:59,890
+في أوروبا اللي كان مهتم جدا في دراسة كتب الطب
+
+524
+00:38:59,890 --> 00:39:07,890
+الإسلامية بيقول انه نسخت الأدوات الجراحية من
+
+525
+00:39:07,890 --> 00:39:12,750
+الكتاب كما هي وعطيتها للمصنعين وصنعونا إياها يعني
+
+526
+00:39:12,750 --> 00:39:16,220
+مرسومة بدقة في الكتبو أعطاها لمثالين صنعوها و
+
+527
+00:39:16,220 --> 00:39:20,620
+أجريت العمليات الجراحية كما أجراها الطبيب الفلاني
+
+528
+00:39:20,620 --> 00:39:25,460
+اللي ذكر اسمه لأ أذكر اسمه أنا فيقول إنه نجحت
+
+529
+00:39:25,460 --> 00:39:28,700
+العملية و استطعت أن أجر العملية بالإنواد و بطريقة
+
+530
+00:39:28,700 --> 00:39:33,000
+تماما لكن المرض مات بعد العملية بانتفاخات و
+
+531
+00:39:33,000 --> 00:39:35,040
+انتهابات ماكانش طبعا أنهم معروفين شوية يعني
+
+532
+00:39:35,040 --> 00:39:38,720
+بكتيريا أيامها و هو بشكل واضح و تكررت العملية حتى
+
+533
+00:39:38,720 --> 00:39:41,880
+ضننتوا بيهم في ظنوم يعني إننا أعتقدت أن هدولة
+
+534
+00:39:41,880 --> 00:39:46,060
+كانوا يعني بتحكوا عينا في المعلومات اللي بعتوافيوم
+
+535
+00:39:46,060 --> 00:39:51,240
+يعني بيقرأ فاتلافت انتباهه انه أشياء ماكانش يركز
+
+536
+00:39:51,240 --> 00:39:53,780
+عليها زي ما احنا بعضنا حاببنا الزبدة بدناش
+
+537
+00:39:53,780 --> 00:40:00,920
+المقدمات و زي ما في كان نكتة بيقول انه اقطعي جزرة
+
+538
+00:40:00,920 --> 00:40:04,240
+وخيارة ومش عارف شو بتعمل طبيخ و بالآخر بيقولك ايه
+
+539
+00:40:04,240 --> 00:40:09,980
+بيقولك اغسل المكونات فهذا
+
+540
+00:40:09,980 --> 00:40:16,070
+عمل العملية عمل عملية كاملة كاملة بحد أثيرهالكن لم
+
+541
+00:40:16,070 --> 00:40:19,430
+يطبق إجراء كان بيقوم في الطي المسلم كونه مسلم كان
+
+542
+00:40:19,430 --> 00:40:24,550
+يتوضأ قبل إجراء العملية الوضوء قبل إجراء العملية
+
+543
+00:40:24,550 --> 00:40:28,050
+غسيل إيدان وغسيل إيدان عبارة عن تطهير تنظيف مظبوط
+
+544
+00:40:28,050 --> 00:40:31,670
+ولا لأ فجال طب بتجرب أنا الموضوع هتجرب الموضوع انه
+
+545
+00:40:31,670 --> 00:40:35,030
+صار يتوضأ وضوء اللي اللي بدي عليه بديه يمشي للآخر
+
+546
+00:40:35,030 --> 00:40:38,730
+و بالفعل بدأت الحالات عنده تتحسن يعني العمليات
+
+547
+00:40:38,730 --> 00:40:42,510
+الجراحية اللي بعد ذلك انه نسبة الوفيات فيها تقل
+
+548
+00:40:42,510 --> 00:40:46,660
+نظرا للانتهابات نبعت العمليةاللي حصل على credit
+
+549
+00:40:46,660 --> 00:40:50,820
+اللي هو Joseph Lester هو التاريخ اكتبوه الله يعني
+
+550
+00:40:50,820 --> 00:40:58,240
+هو المنبسطين هذا المُركّب .. هذا المُركّب دخل
+
+551
+00:40:58,240 --> 00:41:04,600
+المستشفيات لكن إله خواص سيّه سموه كاوية caustic
+
+552
+00:41:04,600 --> 00:41:08,340
+قابضة تمام؟ زي السودا الكاوية تمام؟ caustic soda
+
+553
+00:41:08,340 --> 00:41:13,420
+فهذا ال .. هذا ال .. الخاصية جاءت من استخدامه يعني
+
+554
+00:41:13,420 --> 00:41:17,860
+أمر غير مرضوب فيه خاصةبعد جهور مراكبات أخرى في
+
+555
+00:41:17,860 --> 00:41:22,240
+الخدمة أحد المراكبات اللي اشتقت دمجوا مراكبين
+
+556
+00:41:22,240 --> 00:41:29,520
+الفينول والكلور فهو يبقى هكسا كلورفين
+
+557
+00:41:29,520 --> 00:41:33,580
+كلورفين اللي هو كلورين مع فينول which is a
+
+558
+00:41:33,580 --> 00:41:36,980
+biphenol with six chlorine atoms is used in
+
+559
+00:41:36,980 --> 00:41:40,200
+germicidal soaps حطوها لما .. لما عملوا الصابون
+
+560
+00:41:40,200 --> 00:41:44,770
+ودخلوا الصابون لحالهكتركيب مش deserved ولا قاتل
+
+561
+00:41:44,770 --> 00:41:47,970
+للبكتيريا، لكن احنا بنشوف في ال .. في ال .. في ال
+
+562
+00:41:47,970 --> 00:41:51,710
+.. في السوق الآن أنه في germicidal soups يضافلها
+
+563
+00:41:51,710 --> 00:41:56,790
+مركبات تمام ليجعلها قاتلة، زي الـ Detol soup لكن
+
+564
+00:41:56,790 --> 00:42:01,210
+بقولنا .. بقولنا أنه هناك قلق من أنه إلو neuro
+
+565
+00:42:01,210 --> 00:42:06,270
+toxicity هذا المركب أظهر أو بعض الدراسات أظهرت أنه
+
+566
+00:42:06,270 --> 00:42:09,870
+ممكن يكون إلو neuro toxicity فأوقف برضه استخدام
+
+567
+00:42:10,540 --> 00:42:15,680
+أنشأ ومركّب جديد من الفينول another phenol derived
+
+568
+00:42:15,680 --> 00:42:21,500
+from this grisol وهو يبقى مثالفينول مثالفينول the
+
+569
+00:42:21,500 --> 00:42:24,880
+active ingredient in lysol اللي هو شبيه بالديترون
+
+570
+00:42:24,880 --> 00:42:30,140
+شبيه بالديترون فينولs not only damage membranes
+
+571
+00:42:30,140 --> 00:42:35,280
+but also damage من البروتين يبقى الأن أن مركبين
+
+572
+00:42:35,280 --> 00:42:40,150
+عرفناهم اللي همالفنولات والكحولات تمام عندهم نقطة
+
+573
+00:42:40,150 --> 00:42:43,150
+على ال destruction لل cell membrane بالإضافة إلى
+
+574
+00:42:43,150 --> 00:42:50,750
+ال proteins الآن بنجي للعنوان التاني في ال
+
+575
+00:42:50,750 --> 00:42:53,310
+mechanism of actions لل disinfectant و ال
+
+576
+00:42:53,310 --> 00:42:57,570
+antiseptic اللي هو ال denaturation أو ال
+
+577
+00:42:57,570 --> 00:43:00,510
+modification of proteins عندنا chlorine, iodine,
+
+578
+00:43:00,590 --> 00:43:02,690
+hedium metals, hydrogen peroxide عندنا
+
+579
+00:43:02,690 --> 00:43:06,450
+formaldehyde و glutaraldehydeEthylene Oxide اللي
+
+580
+00:43:06,450 --> 00:43:09,770
+مار معانا في أول المحاضرة لما نتكلم عن غاز
+
+581
+00:43:09,770 --> 00:43:14,370
+المستخدم في التعقيم وفي Acid and Alkali طبعا ال
+
+582
+00:43:14,370 --> 00:43:17,630
+Acid and Alkali ممكن تشتغل بأكتر من طريقة ال pH
+
+583
+00:43:17,630 --> 00:43:21,590
+نفسه يعمل suppression أو انه هو نفسه يكون عنده
+
+584
+00:43:21,590 --> 00:43:24,670
+mechanism of killing زي البنزوات ال sodium ان
+
+585
+00:43:24,670 --> 00:43:28,350
+تستخدمها كمادة حافظة في بعض المربيات المنزلية اللي
+
+586
+00:43:28,350 --> 00:43:32,110
+تمالوهاأو بعض المخللات المنزلية بتعملوا كرامة
+
+587
+00:43:32,110 --> 00:43:34,190
+الفيروس بتستخدم اللي هو ال Sodium Benzoate
+
+588
+00:43:34,190 --> 00:43:37,710
+وبيشتروها من الصيدليات كمادة بيقولوا عنها
+
+589
+00:43:37,710 --> 00:43:42,810
+البنزوات، همام؟ ال Sodium وهي مادة حافظة تنحل
+
+590
+00:43:42,810 --> 00:43:46,730
+وتكون Benzoic Acid، في الآخر هي إيه؟ عبارة عن
+
+591
+00:43:46,730 --> 00:43:54,110
+Acid، Organic Acid طيب، ال chlorine و ال Iodine
+
+592
+00:43:54,110 --> 00:44:00,600
+بينتموا إلى ال halogen's both haveيعني
+
+593
+00:44:00,600 --> 00:44:04,220
+antibacterial أو antimicrobial effect لكن
+
+594
+00:44:04,220 --> 00:44:08,920
+استخدامتهم مختلفة مثلا انتوا تشربوا الماء مخلوط
+
+595
+00:44:08,920 --> 00:44:12,360
+بمين؟ بالكلورين بس انتوا مش تشربوا الماء مخلوط
+
+596
+00:44:12,360 --> 00:44:15,800
+بالايودين صح ولا لأ؟ هذا يستخدم لغرض و هذا يستخدم
+
+597
+00:44:15,800 --> 00:44:21,100
+لأغراض مختلفة تعالوا نطلع على الكلورين كلورين is
+
+598
+00:44:21,100 --> 00:44:26,020
+used as a disinfectant to purify the water supply
+
+599
+00:44:26,020 --> 00:44:30,390
+and to treat swimming poolsأنتوا تعرفوا أن في قطاع
+
+600
+00:44:30,390 --> 00:44:34,730
+غزة we are blessed by a plenty of areas where
+
+601
+00:44:34,730 --> 00:44:38,770
+swimming pools are common يعني الآن صار فينا مناطق
+
+602
+00:44:38,770 --> 00:44:42,050
+متنوعة مختلفة حتى في بعض المنازل صار فيها swimming
+
+603
+00:44:42,050 --> 00:44:45,870
+pools اللي عنده قطعة أرض صغيرة بيعمله قط .. لكن
+
+604
+00:44:45,870 --> 00:44:51,790
+للأسف كثير من هؤلاء الناس ما .. مش فاهمين إيش يعني
+
+605
+00:44:51,790 --> 00:44:59,090
+swimming pooldifferent persons بيدخلوا المكان هذا
+
+606
+00:44:59,090 --> 00:45:04,930
+مستوى النظافة عندهم غير معروف لجميع تمام؟ من مصادر
+
+607
+00:45:04,930 --> 00:45:10,150
+مختلفة قد تتلوت المياه هناك فلابد من continuous
+
+608
+00:45:10,150 --> 00:45:15,170
+change أو disinfection للwater فالكلورين أحد أهم
+
+609
+00:45:15,170 --> 00:45:20,430
+المركبات كمان شغل مهمة احنا المياه في قطاع غزة
+
+610
+00:45:20,430 --> 00:45:25,240
+مصدرها الأساسي المياه الجوفيةغالبا المياه الجوفية
+
+611
+00:45:25,240 --> 00:45:30,640
+لإن الماء بنزل و بتفلتر و بصل لخزان الجوفي، بتفلتر
+
+612
+00:45:30,640 --> 00:45:34,380
+عبر سبعين، ستين، خمسين متر من الرمل و الطين و
+
+613
+00:45:34,380 --> 00:45:40,360
+الحصر، فبنزل pure تحت، نقي جدا، لكن لما بنسحب و
+
+614
+00:45:40,360 --> 00:45:45,060
+احنا و بنوزع في الشبكة، تمام؟ مابنعرفش الشبكة إيش
+
+615
+00:45:45,060 --> 00:45:52,180
+فيهاطب روحه هم حاطين dosage من ال chlorine بحيث
+
+616
+00:45:52,180 --> 00:45:56,900
+يصلك انت في المنزل تمام في ما يسمى بال residual
+
+617
+00:45:56,900 --> 00:46:02,120
+chlorine ال residual يعني كمية المتبقية مسموح
+
+618
+00:46:02,120 --> 00:46:06,500
+بيها، مسموح بيها يعني غير ضارة للإنسان حتى يقتل أي
+
+619
+00:46:06,500 --> 00:46:10,080
+ميكروبات قد تعلق في البكتيريا من الشبكة طيب بلاش
+
+620
+00:46:10,080 --> 00:46:14,050
+احنا هنا ground water نظيفطب تعالى نروح على دولة
+
+621
+00:46:14,050 --> 00:46:16,970
+أخرى معتمدة على ال surface water و لا افترض نهر
+
+622
+00:46:16,970 --> 00:46:20,970
+النيل و نهر النيل يمكن شوفته انتوا بعض المناظر هنا
+
+623
+00:46:20,970 --> 00:46:24,510
+ال youths بتكون طافية لحيوانات في ناس بتغسل على
+
+624
+00:46:24,510 --> 00:46:29,950
+ضفاف النهر يعني بيحتوي على كام كبير من الملوثات
+
+625
+00:46:29,950 --> 00:46:35,970
+فكيف لنا أن نقول ان هذا الماء صار للشرب؟ بدنا
+
+626
+00:46:35,970 --> 00:46:40,150
+ندخله .. بدنا نشيل المواد العالقة فيه و هذه خطوة
+
+627
+00:46:40,150 --> 00:46:42,550
+بيجون فيها الأخوة البيئيينو بعدين نعمله
+
+628
+00:46:42,550 --> 00:46:46,130
+disinfection .. و بعدين نعمله disinfection ب ..
+
+629
+00:46:46,130 --> 00:46:49,850
+برضه ال chlorine .. بسبب ال chlorine أداء مهمة جدا
+
+630
+00:46:49,850 --> 00:46:54,630
+جدا .. كمان شغلة ال waste water .. المياه العادمة
+
+631
+00:46:54,630 --> 00:46:59,250
+.. لما أحنا نتخلص منها في البحر .. الأصل قبل أن
+
+632
+00:46:59,250 --> 00:47:03,650
+نتخلص منها في البحر .. تمام .. ينحط معاها برضه ال
+
+633
+00:47:03,650 --> 00:47:07,860
+chlorineعشان يقتل الميكروبات لإن احنا .. احنا
+
+634
+00:47:07,860 --> 00:47:11,480
+بنتخلص من المجاري في البحر و في الصيف بروح نلحق
+
+635
+00:47:11,480 --> 00:47:20,460
+للمجاري تبعتنا هذه طب عنواني الأمر مابيخلاش من بعض
+
+636
+00:47:20,460 --> 00:47:23,760
+.. مابيخلاش من بعض ال .. ال يعني خدنا نقول الأشياء
+
+637
+00:47:23,760 --> 00:47:27,900
+الإشبارية معظم دول العالم بتتخلص مني فهي تبعتها في
+
+638
+00:47:27,900 --> 00:47:31,220
+الأجسام بتاعة المائية .. الأجسام المائية في البحار
+
+639
+00:47:32,260 --> 00:47:36,320
+حتى نقلل الضرر، بنقوم بعملته اللي هو الـ
+
+640
+00:47:36,320 --> 00:47:39,040
+disinfection أو المعالجة، بيسموها المعالجة
+
+641
+00:47:39,040 --> 00:47:42,680
+الثالثية وبعض الدول الآن أصبحت ال wastewater
+
+642
+00:47:42,680 --> 00:47:48,540
+بالنسبة لكنزي strategy ممنوع يتضخه في البحر وإنما
+
+643
+00:47:48,540 --> 00:47:53,000
+يجب أن نستخدم لرأي المزرعة تونس دولة رائدة من
+
+644
+00:47:53,000 --> 00:47:56,300
+الدول الرائدة اللي بدأت تجربة رائدة فيه إنها تسقي
+
+645
+00:47:56,300 --> 00:48:01,540
+الحدائق العامة باستخدام المياه المعالجةمعالجة
+
+646
+00:48:01,540 --> 00:48:07,580
+ثالثية يعني فيها نزال العلق والبكتيرين نكمل المرة
+
+647
+00:48:07,580 --> 00:48:08,380
+القادمة ان شاء الله
+
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/b5cF6_LhnGA_raw.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/b5cF6_LhnGA_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..8a8ef059a350194e9df80db0b803f0295617bbc7
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/b5cF6_LhnGA_raw.srt
@@ -0,0 +1,2312 @@
+1
+00:00:21,670 --> 00:00:24,070
+بسم الله الرحمن الرحيم اليوم موضوع جديد ان شاء
+
+2
+00:00:24,070 --> 00:00:28,450
+الله bacterial genetics موضوع ممتع جدا و فيه
+
+3
+00:00:28,450 --> 00:00:32,570
+معلومات قد تساعدنا في فهم كثير من الأمور و
+
+4
+00:00:32,570 --> 00:00:36,410
+المعلومات أيضا لها تطبيقات جيدة في مجال ال
+
+5
+00:00:36,410 --> 00:00:42,580
+clinical and medical microbiomeفده هو الفصل الرابع
+
+6
+00:00:42,580 --> 00:00:46,580
+ان شاء الله bacterial genetics نذكركم بس بإيش
+
+7
+00:00:46,580 --> 00:00:49,900
+المحتوى الوراثي للبكتيريا، يعني البكتيريا إيش فيها
+
+8
+00:00:49,900 --> 00:00:53,840
+نواد وراثية؟ لجينا فيها chromosome وهو single،
+
+9
+00:00:53,840 --> 00:00:58,800
+ضعيف لعب، ولكن circular، بعض البكتيريا اللي لجينا
+
+10
+00:00:58,800 --> 00:01:04,290
+فيها plasmidsبالظبط ال extra chromosomal elements
+
+11
+00:01:04,290 --> 00:01:07,910
+اللي هي سمنها self-replicating و it replicates
+
+12
+00:01:07,910 --> 00:01:10,570
+independently و كلمة independently في التعريف اللي
+
+13
+00:01:10,570 --> 00:01:14,390
+قبلنا مكتوبة بشكل autonomous autonomous يعني ذاتي
+
+14
+00:01:14,390 --> 00:01:18,410
+يعني فيها استقلالية فيها استقلالية المصطلح epizome
+
+15
+00:01:18,410 --> 00:01:23,530
+مر معانا بس ممرش بهذا المسمة فاكرين لما قلنالكوا
+
+16
+00:01:23,530 --> 00:01:28,130
+أن ال plasma قد ترتبط بالكرومزوم و أن الكرومزوم
+
+17
+00:01:28,130 --> 00:01:33,390
+ممكن ينتقل كله إلى بكتير أخرىالبلازميد اللى بترتبط
+
+18
+00:01:33,390 --> 00:01:37,450
+بال chromosome اسمها ابيزوم يعني الابيزوم عبارة عن
+
+19
+00:01:37,450 --> 00:01:40,850
+ايش؟ عبارة عن بلازميد متطابطة بيه اللى هو نعم
+
+20
+00:01:40,850 --> 00:01:43,430
+integrated في ال bacterial chromosome بال
+
+21
+00:01:43,430 --> 00:01:49,050
+chromosome لو
+
+22
+00:01:49,050 --> 00:01:55,810
+حد أمامكوا بتوضحلنا نقطة مهمة جدا جدا اسم فطر اسم
+
+23
+00:01:55,810 --> 00:02:00,660
+واحدخمسة mutation خمسة mutation طلعوا على الصورة
+
+24
+00:02:00,660 --> 00:02:04,360
+أو على الصورة هتلاقوا أنه ألوانهم الخمسة مختلفة
+
+25
+00:02:04,360 --> 00:02:09,400
+على الرغم من أنهم نفس الإسم اللي حدث فيهم mutation
+
+26
+00:02:09,400 --> 00:02:14,030
+طفيف يعني صار فيه تغيير في المادة الوراقيةوبالتالي
+
+27
+00:02:14,030 --> 00:02:19,390
+بدلاش نهمل أي تغيير بيحدث في البكتيريا أو
+
+28
+00:02:19,390 --> 00:02:23,630
+الفيروسات لأن أي تغيير طفيف في ال genotype قد يؤدي
+
+29
+00:02:23,630 --> 00:02:27,710
+إلى تغير في ال phenotype والأن واجب علينا نوضح مع
+
+30
+00:02:27,710 --> 00:02:33,570
+معناه genotype و phenotype بسأل سؤال .. سؤال
+
+31
+00:02:33,570 --> 00:02:38,670
+افتراضي لو أن هناك مخلوقين متطابقين في المادة
+
+32
+00:02:38,670 --> 00:02:43,990
+الوراثيةمتطابقين زي بكتيريا انقسمت، بكتيريا انقسمت
+
+33
+00:02:43,990 --> 00:02:49,650
+فهي متطابقة، هذا النسخة نفس النسخة، هل هناك شرط ان
+
+34
+00:02:49,650 --> 00:02:53,470
+يكونوا ال phenotype نفس الشكل، يعني الملاخق
+
+35
+00:02:53,470 --> 00:02:58,530
+بيحملوا نفس الصفات الخارجية؟ لأ، ليش لأ؟ مدام
+
+36
+00:02:58,530 --> 00:03:02,650
+المادة الوراثية واحدة، لازم يكونوا نفس الشيء، و لا
+
+37
+00:03:02,650 --> 00:03:03,530
+لأ؟
+
+38
+00:03:06,150 --> 00:03:11,630
+لأنه أعطيكم إثارة بسيطة جدا من أنفسكم، الآن أنتوا
+
+39
+00:03:11,630 --> 00:03:15,850
+عندكم خلايا في العين و خلايا في الجلد و خلايا في
+
+40
+00:03:15,850 --> 00:03:19,770
+الكبد و خلايا في القلب، كلهم بيحملوا نفس المادة
+
+41
+00:03:19,770 --> 00:03:24,730
+الوراتية، لكن هذه خلية قلب cardiacو هذه خلية nerve
+
+42
+00:03:24,730 --> 00:03:29,110
+و هذه خلية eye و هذه خلية skin بيختلفوا تماما عن
+
+43
+00:03:29,110 --> 00:03:32,750
+بعضهم البعض ما هو سبب الاختلاف أنه في عندي switch
+
+44
+00:03:32,750 --> 00:03:36,130
+on و switch off لبعض الجينات يعني جينات مطفقة
+
+45
+00:03:36,130 --> 00:03:42,510
+وجينات مشغلة أعطيكم مثال تاني أوضح لو أنا عندي
+
+46
+00:03:42,510 --> 00:03:47,210
+أربع أو خمس أنواع من الإضاءات يعني ضوء أحمر أخضر
+
+47
+00:03:47,210 --> 00:03:52,770
+أزرق أصفر نفس النسخة اللي معايا معاكم أنتمهل
+
+48
+00:03:52,770 --> 00:03:56,910
+بالضرورة نحصل على نفس الإضاءة مع بعضنا؟ طب لو أنا
+
+49
+00:03:56,910 --> 00:04:01,370
+طفيت اللامبة الزرقية و أنتوا خلتوا اللامبات كلهم
+
+50
+00:04:01,370 --> 00:04:06,510
+ضوين يبقى ليس بالضرورة تشابه ال genotype أن يؤدي
+
+51
+00:04:06,510 --> 00:04:13,110
+إلى تشابه ال phenotype قد يبدو .. قد يبدو ال
+
+52
+00:04:13,110 --> 00:04:16,630
+genotype متطابق أو قد .. قد يكون متطابق لكن ال
+
+53
+00:04:16,630 --> 00:04:22,600
+phenotype مرة مختلفة طيباللي خلانا نتكلم عن هذا
+
+54
+00:04:22,600 --> 00:04:26,640
+الموضوع ان احنا بنقول ان البكتيريا بتتغير باستمرار
+
+55
+00:04:26,640 --> 00:04:31,100
+و المحاضرة السابقة لما تحدثنا عن تغيير الأسماء لما
+
+56
+00:04:31,100 --> 00:04:33,900
+قولنا ان البكتيريا بتسمى بال Binomial System Genes
+
+57
+00:04:33,900 --> 00:04:37,620
+و Species و جيبنا بعض الأمثلة قولنا تغيرت أسماءها
+
+58
+00:04:37,620 --> 00:04:40,320
+ياسينيابيستس كان اسمها Pastoreliopistis
+
+59
+00:04:40,320 --> 00:04:45,700
+انتروباكترساكزاكي كان اسمها كرونوباكترساكزاكي طيب
+
+60
+00:04:45,700 --> 00:04:49,520
+هذا التغيير احنا عزوناه المحاضرة الماضية لأن احنا
+
+61
+00:04:49,520 --> 00:04:54,270
+بنعرفشتعلمنا جديدًا صنعنا تقنيات جديدة عرفنا
+
+62
+00:04:54,270 --> 00:04:57,190
+معلومات جديدة عن البكتيريا فغيرناها طب ما بالك أن
+
+63
+00:04:57,190 --> 00:05:01,550
+البكتيريا ممكن تتغير أصلا من صفتها؟ وهذا حقيقة و
+
+64
+00:05:01,550 --> 00:05:05,730
+واقع أن البكتيريا ممكن تتغير من صفتها وأسهل مثال
+
+65
+00:05:05,730 --> 00:05:09,510
+.. يعني خلّيني أقول أوضح مثال بكتيريا بقت
+
+66
+00:05:09,510 --> 00:05:14,100
+sensitive للبنسالين صارت resistant للبنسالينهل ليس
+
+67
+00:05:14,100 --> 00:05:17,740
+هذا تغير في الصفات؟ بكتيريا لما تكون ممرضة و أصبحت
+
+68
+00:05:17,740 --> 00:05:21,980
+ممرضة، هل ليس هذا تغير في الصفات؟ إذا ما الذي يغير
+
+69
+00:05:21,980 --> 00:05:27,100
+الصفات الظاهرية هو تغيير جيني، تغيير الجيني، الآن
+
+70
+00:05:27,100 --> 00:05:30,480
+كيف للبكتيريا أن تتغير جينيا؟ طلعوا اللوحة اللي
+
+71
+00:05:30,480 --> 00:05:36,400
+قدامكم، في عندي gene mutation، طفرات، و الطفرات
+
+72
+00:05:36,400 --> 00:05:41,790
+نوعانان هو spontaneous بمعنى انه تلقاء من تلقاء
+
+73
+00:05:41,790 --> 00:05:45,970
+ذات البكتيريا بتعمل ال mutation و هتقولك رقم و بدك
+
+74
+00:05:45,970 --> 00:05:51,390
+تقولولي الرقم هذا كبير ولا صغير فرصة حدوث mutation
+
+75
+00:05:51,390 --> 00:05:57,510
+تلقائية في البكتيريا واحد في كل مائة مليون قليل
+
+76
+00:05:57,510 --> 00:06:03,430
+كرقملكن لو تخيلنا أن البكتيريا .. الخلية الواحدة
+
+77
+00:06:03,430 --> 00:06:07,330
+.. الخلية الواحدة قد تنقسم إلى مائة مليون أصلا في
+
+78
+00:06:07,330 --> 00:06:10,450
+يوم واحد فبالتالي .. فمعناته كتير أما لو قولنا
+
+79
+00:06:10,450 --> 00:06:15,810
+الرقم هذا في الإنسان .. بيقول عنه قليل لإن صورة
+
+80
+00:06:15,810 --> 00:06:18,890
+الانتقالات أن الإنسان بطيئة جدا بالمقارنة مع مين؟
+
+81
+00:06:18,890 --> 00:06:22,870
+مع البكتيريا إذن الرقم اللي ذكرته إن كان يبدو قليل
+
+82
+00:06:22,870 --> 00:06:27,390
+لكن هو رقم ضخم جدا جدا جدا في البكتيريا وصلت صورة؟
+
+83
+00:06:27,390 --> 00:06:31,780
+طيبالـ spontaneous mutation بيعزوها العلماء إلى
+
+84
+00:06:31,780 --> 00:06:36,800
+أخطاء في عملية الـ replication أساسا يعني أنتوا
+
+85
+00:06:36,800 --> 00:06:41,080
+عارفين ال .. ال .. ال chromosome عشان ينقسم أو
+
+86
+00:06:41,080 --> 00:06:45,280
+يصير ال DNA replication بدنا بعض ال enzymes منها و
+
+87
+00:06:45,280 --> 00:06:47,540
+نفطر ال .. ال .. ال DNA polymerase ال DNA
+
+88
+00:06:47,540 --> 00:06:51,890
+polymeraseهناك نسبة خطأ لل DNA polymerase نسبة خطأ
+
+89
+00:06:51,890 --> 00:06:55,230
+لل DNA polymerase وبالتالي بيحدث أخطاء وبالتالي
+
+90
+00:06:55,230 --> 00:06:59,390
+بيصير لنا ال mutation سميناها spontaneous سميناها
+
+91
+00:06:59,390 --> 00:07:03,890
+spontaneous لإنه تحدث بدون سبب ماعناش سبب معين
+
+92
+00:07:03,890 --> 00:07:08,750
+اللي نقول والله صارت ال mutation بسببه عكسها هيكون
+
+93
+00:07:08,750 --> 00:07:17,030
+إيش induced induced يعني بفعل فاعل كلكم بتعرفوا إن
+
+94
+00:07:17,030 --> 00:07:22,590
+ال ultraviolet lightال UV قد تسبب skin cancer اللي
+
+95
+00:07:22,590 --> 00:07:26,050
+هان تتكلم عن إيه دخل ال cancer و ال mutation كل ..
+
+96
+00:07:26,050 --> 00:07:31,990
+كل cancer أساسه mutation مش بالضرورة اخدوا بالكوا
+
+97
+00:07:31,990 --> 00:07:36,050
+ان كل mutation تؤدي ل cancer بس كل cancer أساسه
+
+98
+00:07:36,050 --> 00:07:41,210
+mutation متفقنا على هذا يمكن اتفقنا على ان ال UV
+
+99
+00:07:41,210 --> 00:07:46,090
+بتحط الخلايا اللي نصل فيها ال mutation و هنعرف هذا
+
+100
+00:07:46,090 --> 00:07:49,600
+الكلامالموضوع الجاي لما اتكلم عن ال control of
+
+101
+00:07:49,600 --> 00:07:53,080
+microorganism بستخدم ال UV في قتل البكتيريا بحدها
+
+102
+00:07:53,080 --> 00:07:59,400
+طبعا يبقى spontaneous و ايش كمان induced طبعا
+
+103
+00:07:59,400 --> 00:08:02,200
+induced بفعل أشياء كثيرة و خاصة في زماننا اللي
+
+104
+00:08:02,200 --> 00:08:05,120
+احنا الآن عايشين فيه كتير من المركبات المصنعة
+
+105
+00:08:05,120 --> 00:08:09,920
+synthetic compounds يعتقد انه و التلوث يعني هناك
+
+106
+00:08:09,920 --> 00:08:16,050
+مياه أنهار الآنيعني تعتبر المياه تبعتها مطفرة تعمل
+
+107
+00:08:16,050 --> 00:08:20,310
+induction ل ال mutation الآن النوع التاني من
+
+108
+00:08:20,310 --> 00:08:25,190
+التغيير الجيني بينتج عن ال gene transfer نقل
+
+109
+00:08:25,190 --> 00:08:31,370
+الجينات من بكتيريا إلى بكتيريا وابتدت طرق رئيسية
+
+110
+00:08:31,370 --> 00:08:36,210
+transformation conjugation and transduction نبدأ
+
+111
+00:08:36,210 --> 00:08:40,980
+بالأولى تخيلوا مع التاني ان دي خلية بكتيريةالخلية
+
+112
+00:08:40,980 --> 00:08:47,340
+البكتيرية عايشة في بيئة منها ممكن تكون ظروف لخلايا
+
+113
+00:08:47,340 --> 00:08:50,800
+أخرى غير ملائمة بدأت موت الخلايا الأخرى أو بلاش
+
+114
+00:08:50,800 --> 00:08:55,780
+خلية بكتيرية أكرمكم الله في ال waste water في
+
+115
+00:08:55,780 --> 00:09:02,250
+المجاري تخيلوا المجاري إيش فيها مواد ورائطيةمن
+
+116
+00:09:02,250 --> 00:09:06,990
+نباتات، من حيوانات، من إنسان، من بكتيريا Free DNA
+
+117
+00:09:06,990 --> 00:09:11,690
+ناتج عن مين؟ عن تحلل المواد الأضياء والخلايا يبقى
+
+118
+00:09:11,690 --> 00:09:17,030
+في عندي abundance وفرة من ال DNA هناك بعض
+
+119
+00:09:17,030 --> 00:09:21,510
+البكتيريا عندها المقدرة علي إنها تاخد ال DNA من
+
+120
+00:09:21,510 --> 00:09:28,550
+المحيط تبعها تسحبه إلى الداخل لما يدخل ال DNAإلى
+
+121
+00:09:28,550 --> 00:09:33,130
+الخلية بيروح و يربطها بال chromosome تبعها كلمة
+
+122
+00:09:33,130 --> 00:09:36,430
+ربطها بال chromosome صار عندي مادة وراثية من
+
+123
+00:09:36,430 --> 00:09:40,250
+مصدرين مختلفين خدوا بالكم فلما نربط مادة وراثية من
+
+124
+00:09:40,250 --> 00:09:42,590
+مصدرين مختلفين نسميها recombination نسميها
+
+125
+00:09:42,590 --> 00:09:45,410
+recombination الكلمة اللي موجودة على اللوحة دي لها
+
+126
+00:09:45,410 --> 00:09:52,510
+recombination إذن لما صار في ارتباط للبكتيريا أو
+
+127
+00:09:52,510 --> 00:09:56,830
+للمادة الوراثية على البكتيريا ال chromosomeو أصبح
+
+128
+00:09:56,830 --> 00:10:03,590
+عندي اكتساب لمادة وراثية جديدة قد تكون ذات فائدة،
+
+129
+00:10:03,590 --> 00:10:06,410
+إيش هي قد تكون ذات فائدة؟ هذه المادة أو القطع اللي
+
+130
+00:10:06,410 --> 00:10:09,130
+حطيناها في البكتيريا الآن أو اللي دخلت البكتيريا
+
+131
+00:10:09,130 --> 00:10:15,650
+لحالها قد تنتج أنزيم قادر على تكسير مادة عضوية
+
+132
+00:10:15,650 --> 00:10:19,710
+البكتيريا لم تكن قادرة قبل ذلك على تكسيرها، فأصبح
+
+133
+00:10:19,710 --> 00:10:25,070
+لديها الآن إيش؟ تنوع، أصبح لديها الآن تنوعفي عملية
+
+134
+00:10:25,070 --> 00:10:27,970
+اللي هو ال .. ال metabolism زادت قدرتها على
+
+135
+00:10:27,970 --> 00:10:31,830
+التغذية طب لما زيدت قدرتها على التغذية على مراكبات
+
+136
+00:10:31,830 --> 00:10:37,610
+متنوعة أليس هذا ميزة إضافية إلها لل survival في ال
+
+137
+00:10:37,610 --> 00:10:41,690
+environment؟ مظبوط و مش مظبوط هيكتسبت صفة جديدة
+
+138
+00:10:41,690 --> 00:10:44,730
+صار تغيير وراتي وماصارش تغيير وراتي؟ صار تغيير إسم
+
+139
+00:10:44,730 --> 00:10:48,110
+العملية كم مرة؟ transformation تعالوا نعبّلها
+
+140
+00:10:48,110 --> 00:10:53,480
+بكلمة علمية الآنit is the free take up of DNA from
+
+141
+00:10:53,480 --> 00:10:58,860
+the surrounding باختصار هو الأخذ الحر أو اكتساب
+
+142
+00:10:58,860 --> 00:11:05,320
+المادة الوراطية من محيط البكتيريا النوع
+
+143
+00:11:05,320 --> 00:11:08,560
+التاني من ال gene transfer هو ال conjugation و ال
+
+144
+00:11:08,560 --> 00:11:13,260
+conjugation مر معانا و شفناه و ال transduction ما
+
+145
+00:11:13,260 --> 00:11:18,420
+مر معانا دعنا نطلع على ال transduction اواطفر وقت
+
+146
+00:11:18,420 --> 00:11:25,120
+و نعرضلكمالـ Presentation يا ريت الأخوة يعرضوننا
+
+147
+00:11:25,120 --> 00:11:34,840
+اللي هو اللي .. اللي بدنا نحطه الآن أمامنا
+
+148
+00:11:34,840 --> 00:11:38,580
+في اللوحة خلية بكتيرية و مكتوب تحتها donor cell
+
+149
+00:11:38,580 --> 00:11:43,100
+يعني خلية متبرعة و في وجهها ال bacteriophage ال
+
+150
+00:11:43,100 --> 00:11:46,480
+bacteriophage هو ال virus اللي بيعمل infection
+
+151
+00:11:46,480 --> 00:11:51,420
+للبكتيري و خدوا بالكم أحد فوائد هذه المحاضرة
+
+152
+00:11:51,990 --> 00:11:57,690
+البكتيلوفاج في فائدتين
+
+153
+00:11:57,690 --> 00:12:01,830
+مهمتين جدا في هذا السياق ان البكتيلوفاج very
+
+154
+00:12:01,830 --> 00:12:09,170
+specific يعني متخصص مش لسلفي الكوكس اوريس لسلالات
+
+155
+00:12:09,170 --> 00:12:14,130
+معينة من السلفي الكوكس اوريسيعني زي ما نقول مانفعش
+
+156
+00:12:14,130 --> 00:12:17,630
+نقول نعمل bacteriophage لبنات سنة تانية في ال ..
+
+157
+00:12:17,630 --> 00:12:21,210
+كل واحدة منهم إلها bacteriophage تخيلوا لو أنا
+
+158
+00:12:21,210 --> 00:12:27,690
+عندي مخزن في المختبر، من ال bacteriophage تبقى X و
+
+159
+00:12:27,690 --> 00:12:32,610
+Y و Z و C و S و N، تمام؟ و قلتني حاجة مجهولة،
+
+160
+00:12:32,610 --> 00:12:37,390
+بكتير مجهولة، مش أنا بقدرمن خلال ال bacteriophage
+
+161
+00:12:37,390 --> 00:12:41,290
+اللي عندي أتعرف عليها، ليش أتعرف عليها؟ لأنه لن
+
+162
+00:12:41,290 --> 00:12:44,330
+يحللها، لن يقتلها ال bacteriophage هذه إلا ال
+
+163
+00:12:44,330 --> 00:12:47,030
+bacteriophage الخاص بيها، وال bacteriophage الآن
+
+164
+00:12:47,030 --> 00:12:50,990
+معلوم لديه، إذا المعلوم ارتبط بالمجهول، فأصبح
+
+165
+00:12:50,990 --> 00:12:55,510
+المجهول لنا معلوم، فهي أول فائدة أنه بنستخدمه في
+
+166
+00:12:55,510 --> 00:12:59,030
+التعرف على ال bacteriophage في المختبر تحت أنواع
+
+167
+00:12:59,030 --> 00:13:03,550
+نسميها phage typing، phage اختصارا ل bacteriophage
+
+168
+00:13:03,550 --> 00:13:09,720
+أو phage typingهي أول فائدة استفدناها من الموضوع،
+
+169
+00:13:09,720 --> 00:13:13,300
+الفائدة التانية، كونه specific وكونه غير مضر
+
+170
+00:13:13,300 --> 00:13:17,320
+للإنسان، لو احنا انجرحنا وصار عندنا اتهاب جروح
+
+171
+00:13:17,320 --> 00:13:22,600
+بستافيلوكوكاس أوريس، ألا يمكن استخدام البكتيروفاج
+
+172
+00:13:22,600 --> 00:13:28,920
+في علاج، في قتل البكتيريا؟والان هناك نشاط محموم
+
+173
+00:13:28,920 --> 00:13:33,760
+جدا في العالم لإنتاج bacteriophages للاستخدام في
+
+174
+00:13:33,760 --> 00:13:37,620
+العلاج، تخيلوا بدل ما نعطي المريض اللي عنده الوز،
+
+175
+00:13:37,620 --> 00:13:41,300
+نعطيه antibiotic إن خليه تهتم ورشله باكتيروفاجة
+
+176
+00:13:41,300 --> 00:13:47,070
+على الوز، امام؟ خارجيلو واحد عنده Salmonella في
+
+177
+00:13:47,070 --> 00:13:50,730
+الأمعاق و أعطيناه Anti Salmonella فاجز يبقى فيه
+
+178
+00:13:50,730 --> 00:13:54,690
+إنه إمكاني أو potential لاستخدام ال bacteriophage
+
+179
+00:13:54,690 --> 00:13:57,490
+في من؟ في العلاج إذن واحدة في ال diagnosis
+
+180
+00:13:57,490 --> 00:14:00,350
+والتانية في العلاج تعالوا نطلع على ال
+
+181
+00:14:00,350 --> 00:14:02,570
+bacteriophage بالأول طبعا فيه كمان فاج ثالثة
+
+182
+00:14:02,570 --> 00:14:08,310
+هنتكلم عنها لاحقا لما نعرف أنواع الفاجز هذا الفاجز
+
+183
+00:14:08,310 --> 00:14:13,400
+اللي احنا شايفينه ع السريةباللحظة انه له tail
+
+184
+00:14:13,400 --> 00:14:19,160
+fibers اللي هم الأطراف المنحنية هذه عشان يقدر
+
+185
+00:14:19,160 --> 00:14:22,980
+يرتبط بالخلية فجر ال cell wall structure و ان ال
+
+186
+00:14:22,980 --> 00:14:27,940
+cell wall structure يوفر يوفر إمكانية ارتباط
+
+187
+00:14:27,940 --> 00:14:32,720
+البكتيريو فهش يبقى مافيش receptor مافيش ارتباط
+
+188
+00:14:32,720 --> 00:14:36,660
+وهذا اللي بيقدي لمين؟ ل specificity لازم يكون فيه
+
+189
+00:14:36,660 --> 00:14:41,140
+receptor للبكتيريو فهشالقادر طبط ال bacteriophage
+
+190
+00:14:41,140 --> 00:14:43,680
+تعالى نشوف إيش بيعمل، بيعمل injection للمادة
+
+191
+00:14:43,680 --> 00:14:47,260
+الوراثية ومعبّر عنها باللون الأخضر، الآن بدأ
+
+192
+00:14:47,260 --> 00:14:52,420
+يتكاثر داخل الخلية وكسّر مين؟ البلازميل، مش
+
+193
+00:14:52,420 --> 00:14:54,800
+البلازميل، هذا ال chromosome، هذا تعبيره عن ال
+
+194
+00:14:54,800 --> 00:14:58,960
+chromosome، الآن لما بيجي بيعبي، طبعا عملية
+
+195
+00:14:58,960 --> 00:15:03,140
+التكاثر في ال bacteriophage هي عملية معقدة، لكن
+
+196
+00:15:03,140 --> 00:15:07,600
+احنا بنفصلها بثلاث مراحل أو أربع مراحلattachment و
+
+197
+00:15:07,600 --> 00:15:10,640
+penetration شفتوا انا لما اترتسق بدخل المادة
+
+198
+00:15:10,640 --> 00:15:14,120
+الوراطية صح؟ هذا بحدثها يعني لو اتطلعوا على عملية
+
+199
+00:15:14,120 --> 00:15:18,820
+نفس هذه عجاز يعني ضخم دخلت المادة الوراطية
+
+200
+00:15:18,820 --> 00:15:22,220
+البكتيريا فاش انا بقول ماحدة بدخل البكتيريا بقولهم
+
+201
+00:15:22,220 --> 00:15:27,640
+انا ال boss انا المسئول I am in charge كل الشغل
+
+202
+00:15:27,640 --> 00:15:31,140
+بتتوجه لخدمتي انا كل كلان يا انزمات يا ريبوزومات
+
+203
+00:15:31,140 --> 00:15:36,710
+يا اي حاجة في الخير at my service ببدأوايعملوله
+
+204
+00:15:36,710 --> 00:15:40,450
+replication و ال transcription و ال translation
+
+205
+00:15:40,450 --> 00:15:45,570
+فاللي بينتج بيكون منتجات فيروسية هذه بيسموها
+
+206
+00:15:45,570 --> 00:15:52,980
+العملية كلهاال .. البكتيروفاج ال reproduction لكن
+
+207
+00:15:52,980 --> 00:15:56,460
+في عملية بعدها اسمها assembly ايش هي assembly؟
+
+208
+00:15:56,460 --> 00:16:01,480
+تجميع هو .. هو صنع الرأس الـ eicosahedra اللي
+
+209
+00:16:01,480 --> 00:16:04,760
+شايفينها زي الصندوق هذه تمام؟ صنع ال tail fibers
+
+210
+00:16:04,760 --> 00:16:07,840
+اللي هم اللي .. اللي هي صنع ال collar العنق تبع
+
+211
+00:16:07,840 --> 00:16:11,940
+البكتيروفاج صنع المادة الوراثية كل الأهم تجمعهم مع
+
+212
+00:16:11,940 --> 00:16:15,920
+بعض عملية تجميع عند عملية التجميع لازم يكون في
+
+213
+00:16:15,920 --> 00:16:20,310
+مادة وراثية صح؟ لل .. للفيروسالمادة الوراثية ماذا
+
+214
+00:16:20,310 --> 00:16:25,230
+بياخدها هو؟ بياخدها من البكتيريا طب البكتيريا الآن
+
+215
+00:16:25,230 --> 00:16:29,610
+عشان توفر مادة وراثية مضطر الفيروس يحلل الكروموزوم
+
+216
+00:16:29,610 --> 00:16:32,690
+تبع البكتيريا فبتكسر الكروموزوم .. الكروموزوم
+
+217
+00:16:32,690 --> 00:16:37,770
+بتكسر فقد يكون هناك قطع من الكروموزوم مساوية في
+
+218
+00:16:37,770 --> 00:16:43,430
+الحجم لقطع من الجينوم تبع الفيروس فبدأ المياه يعبي
+
+219
+00:16:44,360 --> 00:16:48,240
+في ال .. في الرأس تبعته بدل ما يعبي قطعة من المادة
+
+220
+00:16:48,240 --> 00:16:51,060
+الورادية تبعته قد يعبي قطعة من مين؟ من ال
+
+221
+00:16:51,060 --> 00:16:55,800
+bacterial chromosome يبقى شكلا هو bacteriophage
+
+222
+00:16:55,800 --> 00:17:01,460
+لكن مضمونا هو .. هو مش bacteria هو .. هو مجرد حامل
+
+223
+00:17:01,460 --> 00:17:05,360
+معاه اللي هو المادة الورادية تعالي نطلع بشكل سريع
+
+224
+00:17:05,360 --> 00:17:11,960
+على الأعمالية دخل
+
+225
+00:17:12,750 --> 00:17:16,310
+واحد بالغلط وكنا من طبعا هو مش واحد في الغلط
+
+226
+00:17:16,310 --> 00:17:20,610
+الطبيعي بيصير مئات الفيروس هذا راح على ال
+
+227
+00:17:20,610 --> 00:17:23,870
+recipient cell بيفكر حاله مسكين بكتيروفاج عن جد
+
+228
+00:17:23,870 --> 00:17:29,310
+بيعمل injection الآن أخد قطعة من مين من ال donor و
+
+229
+00:17:29,310 --> 00:17:32,130
+أداها ال recipient صار لها integration في اللي هو
+
+230
+00:17:32,130 --> 00:17:35,990
+في ال recipient نكمل مع بعض هذا الآن بالبطيء
+
+231
+00:17:35,990 --> 00:17:41,050
+العملية together
+
+232
+00:17:41,050 --> 00:17:41,910
+assembly
+
+233
+00:17:44,530 --> 00:17:47,170
+البكتيروفاجي اللي قولنا بالغلط خدوا بقى كلمة
+
+234
+00:17:47,170 --> 00:17:52,490
+بالغلط بتعطينا .. بتعطينا نقطة مهمة جدا في المسمة
+
+235
+00:17:52,490 --> 00:17:59,250
+إنه أي قطعةقد تدخل بالخطأ وبالتالي ما بنعرفش
+
+236
+00:17:59,250 --> 00:18:02,890
+القطعة اللى انتقلت اى قطعة ولا انه جين فبسموه هذا
+
+237
+00:18:02,890 --> 00:18:05,750
+النوع من ال transduction اقرأوا على عنوانه هتلاقوا
+
+238
+00:18:05,750 --> 00:18:10,590
+مكتوب generalized transduction عام مش متخصص
+
+239
+00:18:10,590 --> 00:18:13,930
+مابنقدرش نتحكم في القطعة اللى بياخدها مين
+
+240
+00:18:13,930 --> 00:18:20,110
+البكتيريو فاجت على عكس نوع تانى طبعا قد نتحكم في
+
+241
+00:18:20,110 --> 00:18:24,350
+القطعة اللى بياخدها البكتيريو فاجتبس خدوا بالكم
+
+242
+00:18:24,350 --> 00:18:31,770
+المحصرة في البكتيريا الأولى لما دخل دخل بكل وضوح
+
+243
+00:18:31,770 --> 00:18:36,830
+جال انا مسئول نعم انا bacterophage و مسئول عن
+
+244
+00:18:36,830 --> 00:18:41,450
+الخلية و اللي بديها بعمله و في الآخر دمر الخلية و
+
+245
+00:18:41,450 --> 00:18:48,310
+طلع منها عمل lysis لذلك بيسموه lytic phageهذا
+
+246
+00:18:48,310 --> 00:18:51,490
+النوع من الفاجلين يسمونه Lightic Fascia إذا المن
+
+247
+00:18:51,490 --> 00:18:56,130
+المسئول عن ال generalized transduction هو ال
+
+248
+00:18:56,130 --> 00:19:00,650
+lightic fascia تعالوا نطلع على نوع تاني من ال
+
+249
+00:19:00,650 --> 00:19:06,090
+transduction و يسمى specialized transduction
+
+250
+00:19:06,090 --> 00:19:14,220
+أحكيلكوا القصة قبل أن نشوف ال animationالان فاكرين
+
+251
+00:19:14,220 --> 00:19:18,960
+لما البكتيروفاج نزل على البكتيريا زي المضالي وعمل
+
+252
+00:19:18,960 --> 00:19:22,560
+injection للمادة الوراثية دخل البكتيروفاج هذا
+
+253
+00:19:22,560 --> 00:19:28,720
+النوع وجالس السلام عليكم ممكن يعني تبسولي مكان في
+
+254
+00:19:28,720 --> 00:19:32,000
+ال chromosome انا مش لاجي مكان اقعد فيه اقعد في
+
+255
+00:19:32,000 --> 00:19:36,660
+هذا المكان فالبكتيريا بتقول اتفضل فيه لمكان خاص
+
+256
+00:19:36,660 --> 00:19:43,140
+بتجسه و بتقول لل genome تبقى واتفضل بتفضلالبكتيريا
+
+257
+00:19:43,140 --> 00:19:46,800
+بتتكثر و هو بتكثر مع البكتيريا كيف؟ بينقسم ال
+
+258
+00:19:46,800 --> 00:19:50,200
+chromosome تبع البكتيريا فبينقسم هو مع ال
+
+259
+00:19:50,200 --> 00:19:53,480
+chromosome يعني لو صار فيه من البكتيريا الأولى
+
+260
+00:19:53,480 --> 00:19:57,900
+مليون نسخة يبقى فيه عنده و هو كمان برضه مليون نسخة
+
+261
+00:19:57,900 --> 00:20:02,360
+صح ولا لأ؟ لأ المشكلة مش هنا المشكلة أنه صحيح
+
+262
+00:20:02,360 --> 00:20:05,220
+اتكثر مع البكتيريا و البكتيريا وفرتله حاضنة محترمة
+
+263
+00:20:05,220 --> 00:20:11,360
+و كويسة إذا شعر أن البكتيريا مهددة هو مش لاجي تاكل
+
+264
+00:20:11,360 --> 00:20:17,050
+البكتيريا مثلابطلع و بقولها انا .. مش انا .. انا
+
+265
+00:20:17,050 --> 00:20:21,730
+ال light اكترشبس المرة هاد ايش عمل هو؟ هو دخل
+
+266
+00:20:21,730 --> 00:20:26,430
+بهدوء و عمل integration و بعد ما تكاثر بفيه ملايين
+
+267
+00:20:26,430 --> 00:20:31,150
+الخلايا تمام؟ سحب نفسه، الان لما بيسحب نفسه بياخد
+
+268
+00:20:31,150 --> 00:20:36,170
+دايما معاه القطعة المجاورة لمين؟ من ال bacterial
+
+269
+00:20:36,170 --> 00:20:39,810
+chromosome فكل مرة لو دخلنا نفس ال bacterial فشل
+
+270
+00:20:39,810 --> 00:20:44,750
+هياخد نفس القطعة فبنسميه specialized transduction،
+
+271
+00:20:44,750 --> 00:20:45,950
+بنشوف مع بعض العمل
+
+272
+00:20:54,980 --> 00:20:58,940
+هي ال Fage DNA وهو Circular غالبا زى ما حكيتلكوا
+
+273
+00:20:58,940 --> 00:21:01,680
+الآن عملية ال integration بالدم بين وبين ال
+
+274
+00:21:01,680 --> 00:21:04,440
+chromosome تبع البكتيريا هى صار فيه ان integration
+
+275
+00:21:04,440 --> 00:21:15,600
+وفي هذه اللحظة مهم جدا نرجع ماعليش ايش
+
+276
+00:21:15,600 --> 00:21:20,220
+اسمه الآن؟لما ال bacteriophage يكون integrated في
+
+277
+00:21:20,220 --> 00:21:23,980
+الخلية بنسميه Prophage خدوا بالكوا الخلية المحتوية
+
+278
+00:21:23,980 --> 00:21:30,460
+على ال Prophage بنسميها Lysogenized Cell يعني خلية
+
+279
+00:21:30,460 --> 00:21:36,220
+فيها .. هتمر المصطلح هتمر معاكوا بعد قريب الخلية
+
+280
+00:21:36,220 --> 00:21:40,660
+اسمها Lysogenized Cell وهدي برضه الفائدة الثالثة
+
+281
+00:21:40,660 --> 00:21:45,920
+اللي حكيناها اليوم عن ال bacteriophageالتانية
+
+282
+00:21:45,920 --> 00:21:52,540
+treatment التالتة pathogenesis تخيلوا
+
+283
+00:21:52,540 --> 00:21:56,980
+أن البكتيريو ال بروفاج هذا بيحمل في طياته جين
+
+284
+00:21:56,980 --> 00:22:02,170
+لتكسينلما البكتيريا بيصير replication لل DNA،
+
+285
+00:22:02,170 --> 00:22:04,730
+بيصير بعد ذلك transcription، إيش بعد ال
+
+286
+00:22:04,730 --> 00:22:09,030
+transcription؟ Translation، فهتصير تنتج ال toxin
+
+287
+00:22:09,030 --> 00:22:14,130
+اللي هو أصلا من وين جاي من البكتيريا و فعشها،
+
+288
+00:22:14,130 --> 00:22:17,890
+بكتيريا ماعنداش مقدرة تنتج toxin، أصلا لديها
+
+289
+00:22:17,890 --> 00:22:23,100
+المقدرة تنتج toxin، أعطيكم مثال عليها؟الـ
+
+290
+00:22:23,100 --> 00:22:27,160
+Cornebacterium Diphtheria اللي بتسبب مرض الـ
+
+291
+00:22:27,160 --> 00:22:31,880
+Diphtheria هذه البكتيريا أساسا غير ممرضة أصبحت
+
+292
+00:22:31,880 --> 00:22:35,780
+ممرضة لما صارت lysogenized يعني لما صار فيها
+
+293
+00:22:35,780 --> 00:22:40,920
+بروفاج عطاها المخدر اللي انتج منه اللي هو الـToxin
+
+294
+00:22:40,920 --> 00:22:46,400
+وعلى فكرة مجموعة ضخمة من البكتيريا الممرضة هي
+
+295
+00:22:46,400 --> 00:22:51,540
+ممرضة لأن فيها بروفاجهي تلاتة افكارات مهمة جدا
+
+296
+00:22:51,540 --> 00:22:54,560
+اليوم أخدناهم عن ال bacteriophage، diagnosis و
+
+297
+00:22:54,560 --> 00:22:59,660
+treatment و في ال pathogenesis، نكمل بروفاج اللي
+
+298
+00:22:59,660 --> 00:23:03,600
+بقى عند بكتيريا بتنقسم جهدها ملايين المرات، عند
+
+299
+00:23:03,600 --> 00:23:10,000
+ظرف معين ال bacteriophage هيبدأ إيه؟ يقرر بده
+
+300
+00:23:10,000 --> 00:23:13,960
+ينفصل، مين أخد معاه؟ قطعة من اللي هو ال
+
+301
+00:23:13,960 --> 00:23:14,800
+bactericromosome
+
+302
+00:23:17,370 --> 00:23:22,350
+بعمل الآن نفس الخطوات اللى عملها مع ال light cycle
+
+303
+00:23:22,350 --> 00:23:27,010
+هاد
+
+304
+00:23:27,010 --> 00:23:35,290
+اللى أنا عملت ال assembly بعمل license للخلية طبعا
+
+305
+00:23:35,290 --> 00:23:38,250
+هاد ال animation أنا هزلكيه ان شاء الله على الموقع
+
+306
+00:23:38,250 --> 00:23:44,040
+و فيه صوت يعني ممكن ان الشرح يكون commandryالأن هو
+
+307
+00:23:44,040 --> 00:23:46,960
+من donor cell را على recipient cell اللي هي
+
+308
+00:23:46,960 --> 00:23:50,860
+المستقبل بيعمل injection للمادة الوراثية دباته و
+
+309
+00:23:50,860 --> 00:23:53,620
+بيصير لها integration الان صار في البكتيريا
+
+310
+00:23:53,620 --> 00:23:59,480
+الجديدة صفات جديدة من مصدرين مختلفين مصدر الأولاني
+
+311
+00:23:59,480 --> 00:24:04,980
+البكتيريا النفاش نفسه قد يكون مصدر و الجزء اللي
+
+312
+00:24:04,980 --> 00:24:08,240
+أخده من البكتيريا الأخرى كمان مصدر آخر فصار عنده
+
+313
+00:24:08,240 --> 00:24:12,960
+الآن تلت مصادر للمعلومات ال originalو ال
+
+314
+00:24:12,960 --> 00:24:15,080
+bacteriophage نفسه البكتيريا نفسنا او البكتيريا
+
+315
+00:24:15,080 --> 00:24:15,960
+نفسنا او البكتيريا نفسنا او البكتيريا نفسنا او
+
+316
+00:24:15,960 --> 00:24:17,880
+البكتيريا نفسنا او البكتيريا نفسنا او البكتيريا
+
+317
+00:24:17,880 --> 00:24:18,160
+نفسنا او البكتيريا نفسنا او البكتيريا نفسنا او
+
+318
+00:24:18,160 --> 00:24:19,000
+البكتيريا نفسنا او البكتيريا نفسنا او البكتيريا
+
+319
+00:24:19,000 --> 00:24:21,740
+نفسنا او البكتيريا نفسنا او البكتيريا نفسنا او
+
+320
+00:24:21,740 --> 00:24:25,540
+البكتيريا نفسنا او البكتيريا نفسنا او البكتيريا
+
+321
+00:24:25,540 --> 00:24:28,900
+نفسنا او البكتيريا نفسنا او البكتيريا نفسنا او
+
+322
+00:24:28,900 --> 00:24:39,040
+البكتيريا نفسنا او البكتيريا نفسنا او البكتيريا
+
+323
+00:24:42,380 --> 00:24:49,420
+أحد تطبيقات الان
+
+324
+00:24:49,420 --> 00:24:53,400
+gene R اختصار ليش R؟ R غالبا في ال .. في ال .. في
+
+325
+00:24:53,400 --> 00:24:56,960
+ال microbial resistance طبعا هنجيله اتصالات مهان
+
+326
+00:24:56,960 --> 00:25:02,900
+أخرى روف على سبيل المثال روف يعني خشن لكن لو
+
+327
+00:25:02,900 --> 00:25:08,440
+البكتيرياالدولار كان فيها resistant gene للبنسالين
+
+328
+00:25:08,440 --> 00:25:12,460
+نفترض للبنسالين ونقلنا بالباتريوفاج عبر
+
+329
+00:25:12,460 --> 00:25:16,360
+transduction تمام هذا ال gene إلى خلية أخرى هذه
+
+330
+00:25:16,360 --> 00:25:19,400
+الخلية كانت sensitive للبنسالين تلقيا هى بتصير
+
+331
+00:25:19,400 --> 00:25:23,880
+resistantالان نقلت من خلية الى خلية احنا حاجب
+
+332
+00:25:23,880 --> 00:25:27,820
+نسميها horizontal gene transfer ايش يعني
+
+333
+00:25:27,820 --> 00:25:32,680
+horizontal؟ قفقي من خلية الى خلية ال vertical هو
+
+334
+00:25:32,680 --> 00:25:36,640
+الانقسام انه خلية تنقسم تبتعد تتود و تترسل نفس
+
+335
+00:25:36,640 --> 00:25:41,680
+الشيء بس ال transduction و ال transformation و ال
+
+336
+00:25:41,680 --> 00:25:44,840
+conjugation يسمى ايش الان؟ horizontal gene
+
+337
+00:25:44,840 --> 00:25:49,140
+transfer كمل
+
+338
+00:25:52,450 --> 00:25:59,250
+كانت البكتيريا resistant، أخدت البكتيروفاج،
+
+339
+00:25:59,250 --> 00:26:04,370
+هيروح عندنا على .. على recipient cell كتانية و
+
+340
+00:26:04,370 --> 00:26:07,370
+يخليها resistant لل antibiotic اللي كان resistant
+
+341
+00:26:07,370 --> 00:26:08,850
+له الخلية الأولى
+
+342
+00:26:13,170 --> 00:26:16,870
+طيب عشان احنا يعني ان شرحنا ال transformation و
+
+343
+00:26:16,870 --> 00:26:23,770
+ماوضعناش عليها اي توضيحات هاي قدامنا توضيح هذه ال
+
+344
+00:26:23,770 --> 00:26:28,730
+competent cell ايش يعني competent cell competent
+
+345
+00:26:28,730 --> 00:26:33,450
+cell عكسها باللغة لل non-competent cell ال non
+
+346
+00:26:33,450 --> 00:26:37,650
+-competent cell هي الخلية الغير جاهزة لا استقبالت
+
+347
+00:26:37,650 --> 00:26:38,050
+يعني
+
+348
+00:26:42,380 --> 00:26:44,720
+خد بالك في المعمل ممكن نخليها competent احنا
+
+349
+00:26:44,720 --> 00:26:51,820
+بالكهربا يعني نكهربها او نحط عليها مواد ملحية
+
+350
+00:26:51,820 --> 00:26:54,780
+ونخليها جاهزة يعني ممكن احنا نعالجها معالجة
+
+351
+00:26:54,780 --> 00:26:59,060
+ونخليها جاهزة ال competence اللي هي نخليها جاهزة
+
+352
+00:26:59,060 --> 00:27:03,800
+لاستقبال المادة دي ورا تعالوا نطلع مع بعض على
+
+353
+00:27:03,800 --> 00:27:04,600
+الفيديو
+
+354
+00:27:08,790 --> 00:27:11,650
+اللي بيورينا ال competent cell و حواليها fragment
+
+355
+00:27:11,650 --> 00:27:15,190
+من ال DNA، يقولنا من مصادر متنوعة و مختلفة، مش
+
+356
+00:27:15,190 --> 00:27:20,050
+ضروري بكتيريا، مش ضروري بكتيريا، من أي حاجة، بيصير
+
+357
+00:27:20,050 --> 00:27:24,650
+intake للمادة الوراثية، وبعدين بيصيرها
+
+358
+00:27:24,650 --> 00:27:26,990
+recombination، اعتقد ان كلمة recombination صارت
+
+359
+00:27:26,990 --> 00:27:28,190
+واضحة، اتحاد
+
+360
+00:27:33,850 --> 00:27:36,610
+صفة جديدة ولا مش صفة جديدة؟ تغيير وراتي ولا مش
+
+361
+00:27:36,610 --> 00:27:40,310
+تغيير وراتي؟ تغيير وراتي طبعا احنا في المختبرات
+
+362
+00:27:40,310 --> 00:27:46,530
+ممكن نكون بالعملية هذه طمعا في إدخال جينات معينة
+
+363
+00:27:46,530 --> 00:27:51,070
+للبكتيريا بغلط تحسينها أو بغلط إنتاج .. منتج معين
+
+364
+00:27:51,070 --> 00:27:54,810
+يعني ممكن نكون بهذه العملية تعالوا نطلع على اللوحة
+
+365
+00:27:54,810 --> 00:27:57,910
+الرائعة هذه اللي قدامنا وخلّيني أشرحها قبل ما
+
+366
+00:27:57,910 --> 00:28:03,290
+أتكلم أو قبل ما أكملهاأنا قولت في عندي R بمعناين،
+
+367
+00:28:03,290 --> 00:28:08,770
+R resistant و هنا R rough، rough عكس smooth،
+
+368
+00:28:08,770 --> 00:28:12,090
+smooth عنها نعم، في بكتيرية اسمها streptococcus
+
+369
+00:28:12,090 --> 00:28:15,890
+pneumonia حاكينا مرة اظنلي عنهاال street gogas
+
+370
+00:28:15,890 --> 00:28:19,070
+pneumonia في منها two variants صينفين واحدة ب
+
+371
+00:28:19,070 --> 00:28:23,210
+capsule واحدة بدون capsule مستعمراتهم على الطبق
+
+372
+00:28:23,210 --> 00:28:27,270
+بيبدو يعني soft smooth اللي لها capsule و اللي
+
+373
+00:28:27,270 --> 00:28:33,590
+مالاش capsule لتبدو soft بسموها rough لما حققنا ال
+
+374
+00:28:33,590 --> 00:28:40,230
+rough في ال mice الفار يعني الفار ماصارله اشي لما
+
+375
+00:28:40,230 --> 00:28:48,110
+حققنا ال s في ال mice مات الفارالـR، الـR non
+
+376
+00:28:48,110 --> 00:28:51,930
+-pathogenic، بقناة أن البكتيريا حيّة، البكتيريا
+
+377
+00:28:51,930 --> 00:28:55,970
+نفسها حيّة،
+
+378
+00:28:55,970 --> 00:28:58,150
+أنا أعرف أن البعض ممكن يتقلل أحيانًا على هو ال
+
+379
+00:28:58,150 --> 00:29:07,230
+injection، لكن الفار مابعرف، الآن حقنّها بالـSاللي
+
+380
+00:29:07,230 --> 00:29:12,110
+هو ال smooth اللي له كابسول الفقر نات و استنتجنا
+
+381
+00:29:12,110 --> 00:29:15,490
+إنه ولود الكابسول virulence factor حكينا عنه لما
+
+382
+00:29:15,490 --> 00:29:21,190
+تكلمنا عن ال structure لل bacterial cell نات الفقر
+
+383
+00:29:21,190 --> 00:29:28,890
+تجربة تانية الحين جبنا جبنا اللي هو ال ال ال ال
+
+384
+00:29:28,890 --> 00:29:33,250
+smooth و قتلناها بالحرارة و حقلناها في الميسر
+
+385
+00:29:38,840 --> 00:29:44,240
+انتوا عملت غبارة، يعني موت او مجموعة، بقى هاجلبة،
+
+386
+00:29:44,240 --> 00:29:49,280
+اللي قال تجلبة الرابعة، اه تبقى كويس، هذا الخطوة
+
+387
+00:29:49,280 --> 00:29:55,260
+الحاسمة، ال rough حية و ال smooth ميتة، heat
+
+388
+00:29:55,260 --> 00:30:03,060
+killed، برضه هيعيش لأنه اه، لأ، هيموت، احنا مصممين
+
+389
+00:30:03,060 --> 00:30:06,340
+التجلبة عشان يموتعشان نثبتلكوا أنه .. عشان
+
+390
+00:30:06,340 --> 00:30:10,080
+نثبتلكوا أنه ال .. ال rough .. ال rough اللي هي
+
+391
+00:30:10,080 --> 00:30:16,120
+الحية أخدت صفات من ال smooth اللي هي الميتة و عبر
+
+392
+00:30:16,120 --> 00:30:19,260
+إيش أخدتها؟ عبر ال transformation لإن لا مافيه
+
+393
+00:30:19,260 --> 00:30:22,600
+بكتيروفاج ولا فيه ply اللي هي ال conjugation اللي
+
+394
+00:30:22,600 --> 00:30:24,860
+بنعرفها ال conjugation وبالتالي شوفوا الآن إيش
+
+395
+00:30:24,860 --> 00:30:29,520
+اللي بصير الفار لما حقنناها بالخلطة هذه اللي هي ال
+
+396
+00:30:29,520 --> 00:30:36,010
+R الحية و ال smooth الميتة ماد الفارعلى عكس توقعك
+
+397
+00:30:36,010 --> 00:30:40,090
+وانت ولمّا عزلنا منه البكتيريا ما عزلنا ال R، إيش
+
+398
+00:30:40,090 --> 00:30:44,450
+عزلنا؟ ال smooth أظن صارت الصورة واضحة الآن إنه
+
+399
+00:30:44,450 --> 00:30:49,310
+something was transferred من ال smooth الميتة إلى
+
+400
+00:30:49,310 --> 00:30:53,930
+الرف الحية جعلتها smooth إيش هذا الانتقال؟ ال gene
+
+401
+00:30:53,930 --> 00:30:58,970
+تبع اللي هو إنتاج ال capsule هذا تجربة على فكرة
+
+402
+00:30:58,970 --> 00:31:02,310
+وإن كانت بسيطة إلا إنه في منها معلومات كبيرة كتير
+
+403
+00:31:02,310 --> 00:31:05,880
+لازمنا نستقيهاإن المادة الواقعية هي ال DNA حقا لدى
+
+404
+00:31:05,880 --> 00:31:09,720
+DNA إنه في تجربة تانية أخد بس ال DNA و حطوه مع ال
+
+405
+00:31:09,720 --> 00:31:15,400
+air level إن ال capsule is a virulence factor صح و
+
+406
+00:31:15,400 --> 00:31:20,500
+ده ده؟ و ده كتير نقطة مهمة أفرجت بين ال stripped
+
+407
+00:31:20,500 --> 00:31:24,140
+coccus pneumonia ال S و ال R القتل و عدم القتل
+
+408
+00:31:24,140 --> 00:31:26,860
+الموت و عدم الموت الإمراض و عدم الإمراض وجوده و
+
+409
+00:31:26,860 --> 00:31:30,200
+عدم وجوده اللى هو ال cash أرجو أنكوا تكونوا
+
+410
+00:31:38,050 --> 00:31:43,290
+عشان احنا مانظمكوش بنوريكم ال conjugation كمان مرة
+
+411
+00:31:43,290 --> 00:31:49,130
+و نأكد على ان احنا شفنا العملية كاملةهذا ال F
+
+412
+00:31:49,130 --> 00:31:53,050
+positive تمام؟ و ال F negative صار فيه بينهم
+
+413
+00:31:53,050 --> 00:31:58,770
+ارتباط بدأ الارتباط يكون اكتر intimate بعدين انتقل
+
+414
+00:31:58,770 --> 00:32:02,850
+ال F positive بلازميت الى ال F negative و صار فيه
+
+415
+00:32:02,850 --> 00:32:06,030
+يعني double strand للتانين ال F negative ايش صارت
+
+416
+00:32:06,030 --> 00:32:11,510
+اللي اعني؟ F positive و بدأت هي تعمل conjugation ل
+
+417
+00:32:11,510 --> 00:32:16,050
+.. ل .. ل .. ل F negative self وهذا اللي هو ال
+
+418
+00:32:16,050 --> 00:32:24,260
+conjugation المعروفالابيزوم، فاكرين الابيزوم؟ هيها
+
+419
+00:32:24,910 --> 00:32:28,210
+اللي هي ال .. ال .. ال .. ال .. ال plasmid متحدة
+
+420
+00:32:28,210 --> 00:32:31,870
+مع مين؟ مع ال chromosome و لاحظوا في ال .. في
+
+421
+00:32:31,870 --> 00:32:34,650
+الخطوة .. في المربع التالت أن ال chromosome .. ال
+
+422
+00:32:34,650 --> 00:32:38,110
+chromosome بدأ ينتقل مع مين؟ مع اللي هو .. مع ال
+
+423
+00:32:38,110 --> 00:32:42,850
+ebisome نفسه و هاد يقولنا ساموها HFR أو high
+
+424
+00:32:42,850 --> 00:32:46,110
+frequency recombination لإنه فينا كمية كبيرة من
+
+425
+00:32:46,110 --> 00:32:49,550
+مين؟ من المادة الوراثية اللي بينتقل مش .. مش زي ال
+
+426
+00:32:49,550 --> 00:32:52,830
+plasmid ال plasmid قطعة صغيرة بالمقارنة مع ال
+
+427
+00:32:52,830 --> 00:32:59,560
+chromosomeيبقى تلت طرق .. تلت طرق من ال gene
+
+428
+00:32:59,560 --> 00:33:04,060
+transfer بدكوا تكونوا عارفينهم بشكل جيد لإنه مهمين
+
+429
+00:33:04,060 --> 00:33:08,340
+جدا في حياتنا بنعرف إن البتكيلة اتغيرت لمجموعة من
+
+430
+00:33:08,340 --> 00:33:12,100
+الأسباب واحد من هذه الأسباب ال mutation السبب
+
+431
+00:33:12,100 --> 00:33:14,660
+التاني الرئيسي ال gene transfer اللي هم التلات
+
+432
+00:33:14,660 --> 00:33:18,840
+اللي ذكرناها ال .. ال .. ال .. الجزء الرابع اللي
+
+433
+00:33:18,840 --> 00:33:20,680
+هو ال .. ال protoplast fusion
+
+434
+00:33:24,970 --> 00:33:28,170
+اعتقد ان المصطلح مش غريب وانا شبحته لان اتكلمت عن
+
+435
+00:33:28,170 --> 00:33:32,450
+ال cell wall لما قلت ان البكتيريا اللى من غير cell
+
+436
+00:33:32,450 --> 00:33:35,750
+wall بنسميها protoplast خاصة اللى بنصنعها احنا
+
+437
+00:33:35,750 --> 00:33:40,090
+وكان فينا صديق يدرس معانا في مدستير فكان احد
+
+438
+00:33:40,090 --> 00:33:46,140
+التجارب طلعته في البحث ان يدمج two cellsال two
+
+439
+00:33:46,140 --> 00:33:50,760
+cells اللي بذكره ان واحدة stable واحدة منهم stable
+
+440
+00:33:50,760 --> 00:33:54,660
+و بتنتج كمية كبيرة من ال antibiotic و التانية
+
+441
+00:33:54,660 --> 00:33:57,720
+بتنتج كمية كبيرة من ال antibiotic بس مش ايه مش
+
+442
+00:33:57,720 --> 00:34:01,490
+stableفالميزة اللي هنا عيب في هذه و العيب اللي هنا
+
+443
+00:34:01,490 --> 00:34:05,230
+أو الميزة اللي هنا عيب في التانية، فقال أو كان
+
+444
+00:34:05,230 --> 00:34:09,170
+بيفكر أنه لو دمجهم اتنتين مع بعض، تمام؟ قد يحصل
+
+445
+00:34:09,170 --> 00:34:13,090
+على خلية تمتلك الصفتين الإيجابيتين، و بالفعل هذا
+
+446
+00:34:13,090 --> 00:34:15,830
+ما حدث في هذا كان يمكن .. يمكن أن كان الأفضل جزء
+
+447
+00:34:15,830 --> 00:34:19,270
+في رسالة feministية تبعه، و العملية بيسميها plot
+
+448
+00:34:19,270 --> 00:34:22,910
+of last في ذلك اللحظةالميزة والعيب كانت نتيجة عن
+
+449
+00:34:22,910 --> 00:34:26,590
+مين؟ عن التركيب الولادة لما احنا اتسبنا الصفتين مع
+
+450
+00:34:26,590 --> 00:34:30,390
+بعض ايه صار عندنا؟ صار عندنا تغيير في الجينات
+
+451
+00:34:30,390 --> 00:34:34,450
+وبالتالي العنوان mechanism of bacterial variation
+
+452
+00:34:34,450 --> 00:34:39,770
+من اين يأتي اختلاف الجينات في اللي هو البكتيريات
+
+453
+00:34:39,770 --> 00:34:43,030
+mutation gene transfer protoplast fusion
+
+454
+00:34:48,820 --> 00:34:53,620
+الـ protoplast fusion عبارة عن two cells، بنشيل ال
+
+455
+00:34:53,620 --> 00:34:55,840
+cell wall تبعهم، اللي كان اللي هم cell wall، تمام؟
+
+456
+00:34:55,840 --> 00:34:59,900
+اللي كان اللي هم cell wall، و بنحط معاهم مادة
+
+457
+00:34:59,900 --> 00:35:04,100
+بسموها fusing agent، زي polyethylene glycol،
+
+458
+00:35:04,100 --> 00:35:09,140
+polyethylene glycol، أو بختصروا PEG، polyethylene
+
+459
+00:35:09,140 --> 00:35:13,500
+glycolهذه المادة بتحب تدمج الخلايا مع بعضها
+
+460
+00:35:13,500 --> 00:35:17,380
+فالخلايا بندمج و بيصيروا بدل ما هم خاليتين بيصيروا
+
+461
+00:35:17,380 --> 00:35:21,720
+خالية واحدة و ال recombination فاكرين كلمة
+
+462
+00:35:21,720 --> 00:35:24,860
+recombination؟ اللي هو ارتباط مادة و راطية من مصدر
+
+463
+00:35:24,860 --> 00:35:28,360
+لمصدر آخر اللي بيشتغل بحيث انه مايكون إلا في
+
+464
+00:35:28,360 --> 00:35:32,860
+كروموزوم واحد في الخلية، في خلاية البكتيريا هذا
+
+465
+00:35:32,860 --> 00:35:35,300
+الكروموزوم غالبا بيكون من مين؟ خليط من ال two
+
+466
+00:35:35,300 --> 00:35:41,230
+cellsفالان صرا عندي خاليتين او كان في عندي خاليتين
+
+467
+00:35:41,230 --> 00:35:47,550
+صاروا خالية واحدة بكرومترا طب احنا شرحنا ال gene
+
+468
+00:35:47,550 --> 00:35:52,850
+transfer و ما شرحنا ال mutation و هنجف عند ال
+
+469
+00:35:52,850 --> 00:35:59,550
+mutation طويلا لانه مهم جدا نعرف نوعين من ال
+
+470
+00:35:59,550 --> 00:36:03,310
+mutation اسمهم base, substitution و حاجة اسمها
+
+471
+00:36:03,310 --> 00:36:11,460
+friendshipو ال frame shift معناها إزاحة و الإزاحة
+
+472
+00:36:11,460 --> 00:36:18,420
+قد تمتج عن إضافة أو حدث إضافة أو حدث و عندي الصحية
+
+473
+00:36:18,420 --> 00:36:22,940
+أنا animation كويسة بتوريكم إياها بتوضح
+
+474
+00:36:28,260 --> 00:36:32,600
+بس هي حابة اتركزوا مع ال presentation اكتر من
+
+475
+00:36:32,600 --> 00:36:37,880
+اتركزوا مع الكتابة لإن الفكرة فيها كتير واضحة، هذه
+
+476
+00:36:37,880 --> 00:36:41,940
+المادة الوراثية مكوّنة من قواعد نيتروجينية، كل
+
+477
+00:36:41,940 --> 00:36:46,860
+ثلاث قواعد تشكل شفرة، ال codon بالظبط، و ال codon
+
+478
+00:36:46,860 --> 00:36:53,410
+يعني شيءلكن الشيء قد يُرمز له بأكتر من كودون، صح؟
+
+479
+00:36:53,410 --> 00:37:01,990
+يعني مثلا الـ stop codons UAA, UAG و UGA، كويسة
+
+480
+00:37:01,990 --> 00:37:04,550
+النقطة دي، لإنه بتفيدنا الآن في ال mutation، بعض
+
+481
+00:37:04,550 --> 00:37:11,210
+الأحيان ال UAG لو اتغيرت ل UAA، صحيح صارت أفرق،
+
+482
+00:37:11,210 --> 00:37:14,510
+لكن هي نفس ال stop codon، و تالي ما صارش أنها أي
+
+483
+00:37:14,510 --> 00:37:16,430
+تغيير، فبسمو ال mutation silent
+
+484
+00:37:25,860 --> 00:37:33,660
+هذا طبعا اللي هو الديانيك اللي مرايز بيشتغل زي
+
+485
+00:37:33,660 --> 00:37:37,740
+ما احنا احنا بعض الأحيان نطبع على الحاسوب و بعدين
+
+486
+00:37:37,740 --> 00:37:42,590
+نرجع ندققال DNA في الملايز عنده editing يعني عملية
+
+487
+00:37:42,590 --> 00:37:45,610
+تحرير للأخطاء إذا كان فيه عنده أخطاء لكن لا يوجد
+
+488
+00:37:45,610 --> 00:37:50,170
+hundred percent proof فقلنالكوا بيصير أخطاء و
+
+489
+00:37:50,170 --> 00:37:55,310
+سمينا الأخطاء دي اللي هي ال spontaneous mutation و
+
+490
+00:37:55,310 --> 00:37:58,050
+أنا بقولي هذه الأخطاء يمكن بيبقى .. بيبقى نشتدرنا
+
+491
+00:37:58,050 --> 00:38:02,280
+احنا كباشر أخطاء قد تكون مبرمجة هتحصللأن احنا
+
+492
+00:38:02,280 --> 00:38:05,640
+بنلاحظ أن ال mutation في البكتيريا غالبا بتفيد
+
+493
+00:38:05,640 --> 00:38:08,860
+البكتيريا، البكتيريا بتستفيد من هذه ال mutations،
+
+494
+00:38:08,860 --> 00:38:13,620
+قد تحسن من قدراتها على ال adaptation مع ال
+
+495
+00:38:13,620 --> 00:38:17,640
+environment، تخيلوا الآن الوضع التالي، احنا في
+
+496
+00:38:17,640 --> 00:38:24,080
+كوكب إنه 150 سنة بنصنع مركبات synthetic، مش
+
+497
+00:38:24,080 --> 00:38:24,560
+natural
+
+498
+00:38:27,100 --> 00:38:30,520
+إيش يعني مش natural؟ يعني .. يعني بمعنى آخر non
+
+499
+00:38:30,520 --> 00:38:36,940
+-biodegradable يعني غير قابل للتكسير كمان 150 سنة
+
+500
+00:38:36,940 --> 00:38:40,500
+من هذه المركبات قد يمتلق كوكبنا .. قد يمتلق كوكبنا
+
+501
+00:38:40,500 --> 00:38:45,820
+بالمخلفات و النفايات تبع هذه المنتجات وجود
+
+502
+00:38:45,820 --> 00:38:49,580
+بكتيريا قادرة على التاكله .. الآن احنا بنعرف إن
+
+503
+00:38:49,580 --> 00:38:51,900
+فيه بكتيريا بتاكل ال plastic و ال plastic
+
+504
+00:38:55,260 --> 00:39:00,160
+Synthetic اكتر من المبيضات الحشرية اللى هى
+
+505
+00:39:00,160 --> 00:39:04,320
+Synthetic أساسا و non-biodegradable بنروح على
+
+506
+00:39:04,320 --> 00:39:08,200
+أماكن رشها اللى وان بيرشوها باستمرار و بناخد
+
+507
+00:39:08,200 --> 00:39:12,840
+بكتيريا من المنطقة هناك تمام و بنحاول نعمل وسط
+
+508
+00:39:12,840 --> 00:39:16,420
+زراعى هذا الوسط الزراعى مافيش فيه مصدر كربوني
+
+509
+00:39:16,420 --> 00:39:19,100
+فاكدوا ان هذا الكربوني لافيش لاكود ولا فيه نشاط
+
+510
+00:39:19,100 --> 00:39:24,670
+بحطلها المبيض مصدر كربونيوبنزرع البكتيريا اللى
+
+511
+00:39:24,670 --> 00:39:29,030
+موجودة من الطربة على الطبق هذا إذا البكتيريا بتكسر
+
+512
+00:39:29,030 --> 00:39:32,550
+المصدر الكربوني حتى إنه بشكل مصدر عموما فبنصير
+
+513
+00:39:32,550 --> 00:39:35,370
+نازل البكتيريا وعزلنا بكتيريا بالفعل اللى مقدرة
+
+514
+00:39:35,370 --> 00:39:39,990
+على تكسير المبدال هذا يمكن يكون نازل عن إيه؟ عن
+
+515
+00:39:39,990 --> 00:39:44,770
+spontaneous فال spontaneous من ورقة نظرنا احنا انه
+
+516
+00:39:44,770 --> 00:39:48,410
+..لأ مش سيء انه ..انه بتون سبب لكن قد يكون
+
+517
+00:39:48,410 --> 00:39:51,790
+البكتيريا مبرمجة ان يصير فيها spontaneous mutation
+
+518
+00:40:04,800 --> 00:40:07,800
+بنكمل نوعين الميمتاجين الـ Bay Substitution اللي
+
+519
+00:40:07,800 --> 00:40:11,740
+هي إحلال لقاعدة و Frame Shift مفيش أنها غير
+
+520
+00:40:11,740 --> 00:40:15,060
+النوعين دول اللي موجودين قدامك الآن بيكونوا طالعوا
+
+521
+00:40:15,060 --> 00:40:17,100
+على اللوحة هذه نعمل stop
+
+522
+00:40:26,980 --> 00:40:33,900
+قدامنا DNA و المقابل تبعاش هكون mRNA و بعد ال mRNA
+
+523
+00:40:33,900 --> 00:40:36,880
+هكون اللي هو ال protein نطلع على ال sequence
+
+524
+00:40:36,880 --> 00:40:40,700
+فينالألانين تيروسين ألانين و أرجانين مظبوط هي
+
+525
+00:40:40,700 --> 00:40:44,160
+الأربعة الموجودين بين العالمين على تتابع الحلقات
+
+526
+00:40:44,160 --> 00:40:48,160
+اللي موجودة في ال DNA و من ثم في اللي هو ال RNA
+
+527
+00:40:48,160 --> 00:40:53,500
+الآن بدنا نشوف إيش تأثير ال base substitution أو
+
+528
+00:40:53,500 --> 00:40:56,040
+احتمالات تأثير ال base substitution
+
+529
+00:40:59,690 --> 00:41:04,630
+نقف أول احتمال اللي هو silent mutation تفكرين ما
+
+530
+00:41:04,630 --> 00:41:08,410
+هو silent mutation؟ اللي يقول عنها انه تغيير ينتج
+
+531
+00:41:08,410 --> 00:41:13,550
+عنه عدم تغيير في الرمز .. مش في الرمز في ال .. في
+
+532
+00:41:13,550 --> 00:41:16,650
+ال .. في ال .. في المرموز لهو في المرموز لهو اللي
+
+533
+00:41:16,650 --> 00:41:20,010
+هو ال amino acid أو إذا كان stop codon إلى آخر
+
+534
+00:41:20,010 --> 00:41:27,710
+وهذا لن يؤثر على تتابعالأمينو assets وبالتالي ال
+
+535
+00:41:27,710 --> 00:41:32,550
+mutation موجودة لكن لم يظهر لها أي تأثير و بيسميها
+
+536
+00:41:32,550 --> 00:41:39,850
+silent mutation النوع التاني لأ ال codon احنا قلنا
+
+537
+00:41:39,850 --> 00:41:43,910
+انه base واحدة بتتغير صح شيلنا base و حطينا base
+
+538
+00:41:43,910 --> 00:41:48,530
+الان لما تغيرت ال base تتغير ال amino acidيبقى صار
+
+539
+00:41:48,530 --> 00:41:53,710
+عندي هانال أنا أعيش هنا لحظة بدل الالانين صار عندي
+
+540
+00:41:53,710 --> 00:41:58,970
+ال glycine يبقى صار فيه اختلاف اخدوا بالكم مش يعني
+
+541
+00:41:58,970 --> 00:42:01,490
+ان صار فيه اختلاف اقل من قصدي يعني الاختلاف بسيط
+
+542
+00:42:01,490 --> 00:42:06,750
+قد يكون الاختلاف drastic يعني بيبقى له تأثير قد
+
+543
+00:42:06,750 --> 00:42:11,050
+يعني يجعل من ال protein non-functional وقد يجعله
+
+544
+00:42:11,050 --> 00:42:16,470
+اقل functional وقد لايؤثر على ال functional البتة
+
+545
+00:42:16,470 --> 00:42:20,440
+يعنيما بنعرفش بالضبط إيش التأثير، لكن في الآخر فيه
+
+546
+00:42:20,440 --> 00:42:26,280
+تأثير، فيه تأثير Non-sense mutation بدل ما يتغير
+
+547
+00:42:26,280 --> 00:42:31,660
+الكودون لرمز الأمينواسد، بتغير ل stop-kodon، فبصير
+
+548
+00:42:31,660 --> 00:42:34,620
+لما يقرأوا ال RNA polymerase عنده يوجّه، وبالتالي
+
+549
+00:42:34,620 --> 00:42:37,940
+إيش ال protein اللي بيطلع هنا؟ protein ناجس، هذا
+
+550
+00:42:37,940 --> 00:42:39,760
+ال protein ناجس إيش بنسميه؟ إنه واحدة من ال
+
+551
+00:42:39,760 --> 00:42:43,740
+mutation non-sense، كلام فاضي، كلام فارغ، تمام؟
+
+552
+00:42:43,740 --> 00:42:48,690
+يعني protein مش تمام، مش تمامهذا الان احنا اللي
+
+553
+00:42:48,690 --> 00:42:52,650
+اتعلمنا اللي هو ال base substitution base
+
+554
+00:42:52,650 --> 00:42:59,770
+substitution يا اما silent يا اما missings او non
+
+555
+00:42:59,770 --> 00:43:03,910
+missings نيجي الان لمين؟ لل frame shift قبل ان
+
+556
+00:43:03,910 --> 00:43:07,490
+ننتقل لل frame shift احنا عندنا سطر من الطالبات
+
+557
+00:43:07,490 --> 00:43:12,050
+اللي امامنا بهذا الشكل واحد اتنين تلاتة اربعة خمسة
+
+558
+00:43:12,050 --> 00:43:17,950
+ستة جبنا كرسي و حطينا بعد لقم اتنينإيش كان ترتيبها
+
+559
+00:43:17,950 --> 00:43:19,850
+التانية؟ كانت ترتيبها التانية الآن إيش صار
+
+560
+00:43:19,850 --> 00:43:23,010
+ترتيبها؟ تلاتة و اللي بعدها كانت تلاتة صارت أربعة
+
+561
+00:43:23,010 --> 00:43:28,050
+يبقى كل ال sequence اللي بعد الإضافة أو بعد الحذف
+
+562
+00:43:28,050 --> 00:43:31,510
+هيتغير هيحسب منها frame shift نوع من إيه؟ من
+
+563
+00:43:31,510 --> 00:43:35,710
+الإزاحة اللي قلنا كانت اتنين صارت تلاتة أو اللي
+
+564
+00:43:35,710 --> 00:43:38,790
+كانت اتنين صارت واحد يعني على حسب اللي هو هل هو
+
+565
+00:43:38,790 --> 00:43:42,850
+addition ولا deletion هل هو insertion ولا deletion
+
+566
+00:43:42,850 --> 00:43:48,630
+وهذا هو ما يسمى بال frame shiftطبعا تخيلوا أنه
+
+567
+00:43:48,630 --> 00:43:52,390
+تغيير واحد أدى إلى تغيير الكل اللي بعده، صح ولا
+
+568
+00:43:52,390 --> 00:43:55,510
+لأ؟ وبتالي غالبا تأثير ال frame shift برضه تأثير
+
+569
+00:43:55,510 --> 00:43:55,990
+قوي
+
+570
+00:44:06,610 --> 00:44:10,130
+تعالوا تطلعوا على التأثير القوي، بس أول amino acid
+
+571
+00:44:10,130 --> 00:44:13,410
+داي زي ما هو، أول amino acid داي زي ما هو ال bag
+
+572
+00:44:13,410 --> 00:44:16,550
+إيش صار فيهم، اتغيروا، سواء كان في ال deletion أو
+
+573
+00:44:16,550 --> 00:44:19,410
+كان في مين، اللي هو ال insertion، يعني بمعنى آخر
+
+574
+00:44:19,410 --> 00:44:24,170
+إن كل الحلقات أو كل القواعد اللي بعد الإضافة أو
+
+575
+00:44:24,170 --> 00:44:28,390
+الحدث، هيتغيروا ال amino acidإذا ال imitation
+
+576
+00:44:28,390 --> 00:44:33,490
+نوعان، base substitution والتانية frame shift
+
+577
+00:44:33,490 --> 00:44:36,330
+imitation، وعندها ان شاء الله بنوقف و بنكمل
+
+578
+00:44:36,330 --> 00:44:37,670
+المحاضرة القادمة ان شاء الله
+
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/f2aiJbJwTRQ.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/f2aiJbJwTRQ.srt
new file mode 100644
index 0000000000000000000000000000000000000000..0d90ae129c899e299dc2386958488c3697d3b4b1
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/f2aiJbJwTRQ.srt
@@ -0,0 +1,2547 @@
+1
+00:00:20,690 --> 00:00:24,770
+بسم الله الرحمن الرحيم الموضوع ما زلنا في ال
+
+2
+00:00:24,770 --> 00:00:29,290
+bacterial infection and immunity وحنعمل quick 
+
+3
+00:00:29,290 --> 00:00:33,410
+revision للما بدأنا بيه المرة الماضية تحدثنا عن
+
+4
+00:00:33,410 --> 00:00:37,590
+ثلاث مصطلحات متعلقة بوصف العلاقة بين كائنين اللي 
+
+5
+00:00:37,590 --> 00:00:40,550
+هم ال commensalism و الparasitism بالإضافة إلى ال
+
+6
+00:00:40,550 --> 00:00:44,030
+mutualism  و أكدنا على أنه الparasitism هي العلاقة
+
+7
+00:00:44,030 --> 00:00:44,730
+الأكثر شيء 
+
+8
+00:00:47,840 --> 00:00:51,260
+تحدثنا عن ال normal microbiota و بعض الفوائد 
+
+9
+00:00:51,260 --> 00:00:56,240
+المتنوعة اللي شملناهم تقريبا هم أربع نقاط لكن كنا
+
+10
+00:00:56,240 --> 00:00:59,780
+عنهم ثلاثة ممكن منهم ثلاثة أفضل الأولى vitamin K
+
+11
+00:00:59,780 --> 00:01:03,300
+رقم اثنين ال colonization resistance و تمثل في
+
+12
+00:01:03,300 --> 00:01:07,420
+شغلتين اللي هو occupying the space and producing
+
+13
+00:01:07,420 --> 00:01:11,620
+chemicals زي ال bacteriocins و تحدثنا عن ان ال
+
+14
+00:01:11,620 --> 00:01:14,220
+normal flora قد تساعد في ال development لبعض ال
+
+15
+00:01:14,220 --> 00:01:17,980
+organs زي ال lymphoid patches اللي عبارة عن مجموعة من
+
+16
+00:01:17,980 --> 00:01:22,360
+ال lymph nodes موردة في ال intestinal tract كمان
+
+17
+00:01:22,360 --> 00:01:25,820
+تطرقنا إلى الفرق ما بين ما يسمى بال hospital
+
+18
+00:01:25,820 --> 00:01:28,640
+infection وما بين اللي هو ال community acquired
+
+19
+00:01:28,640 --> 00:01:33,740
+infection وحاولنا أن نؤكد على مفهوم أن الميكروبات
+
+20
+00:01:33,740 --> 00:01:38,360
+المكتسبة في ال health care institutions are usually
+
+21
+00:01:38,360 --> 00:01:41,960
+more resistant to antibiotics than those acquired
+
+22
+00:01:41,960 --> 00:01:46,130
+in the community هذه كانت المفهوم العام وأن هناك
+
+23
+00:01:46,130 --> 00:01:49,270
+قد يحدث تغيير في ال normal microbiota ما بين
+
+24
+00:01:49,270 --> 00:01:53,150
+الموجود في المجتمع وبين الموجود في هذه المؤسسة
+
+25
+00:01:53,150 --> 00:01:58,570
+وهذه اللوحة كانت تلخيصًا لهذا الكلام ويبدأ بالحديث
+
+26
+00:01:58,570 --> 00:02:01,230
+عن مصطلحات مثل pathogen و non pathogen و
+
+27
+00:02:01,230 --> 00:02:05,650
+opportunistic pathogen و pathogenic الآن ال
+
+28
+00:02:05,650 --> 00:02:08,510
+pathogen هو من يمتلك ال pathogenicity وال non
+
+29
+00:02:08,510 --> 00:02:11,790
+pathogenic هو من لا يمتلك ال pathogenicity ال
+
+30
+00:02:11,790 --> 00:02:15,070
+opportunistic pathogen هو يمتلك ال pathogenicity 
+
+31
+00:02:15,070 --> 00:02:18,470
+لما يكون الشخص اللي أمامه immunocompromised أما
+
+32
+00:02:18,470 --> 00:02:23,210
+لما يكون immunocompetent فلا يستطيع أن يحدث هذا
+
+33
+00:02:23,210 --> 00:02:29,050
+المرض نطلق على مصطلح اسمه virulence يقول أن هو
+
+34
+00:02:29,050 --> 00:02:33,070
+عبارة عن quantitative measure مقياس كمي لمدى
+
+35
+00:02:33,070 --> 00:02:37,530
+القدرة على إحداث مرض في جسدنا إما بال LD50 أو ال ID50
+
+36
+00:02:37,530 --> 00:02:43,150
+وشرحنا أن ال ID50 هي الجرعة اللازمة لإحداث عدوى
+
+37
+00:02:43,150 --> 00:02:47,810
+عند 50% من حيوانات التجارب أو ال test animal LD50
+
+38
+00:02:47,810 --> 00:02:53,950
+بدل ما هي infection بتسبب وفيات أو قتل أعتقد أنها
+
+39
+00:02:53,950 --> 00:03:00,410
+واجهتني في نفسي عند اللوحة هذه أحد أهم العناصر اللي
+
+40
+00:03:00,410 --> 00:03:03,270
+بتساهم في ال virulence حاجة اسمها adherence
+
+41
+00:03:03,270 --> 00:03:06,550
+ولاحظوا ال adherence أنا معلم على ثلاث كلمات في
+
+42
+00:03:06,550 --> 00:03:10,050
+اللوحة adherence, adhesion أو attachment كلهم
+
+43
+00:03:10,050 --> 00:03:14,990
+بيبقى نفس المعنى المهم أن نفهم أن ال adhesion
+
+44
+00:03:14,990 --> 00:03:21,130
+غالبا specific إيش إن specific يعني مركب ما مركب
+
+45
+00:03:32,630 --> 00:03:37,070
+الانتشار هو مفهومه 
+
+46
+00:03:37,070 --> 00:03:41,450
+أن الميكروب قادر على الانتقال من مكان الإصابة إلى
+
+47
+00:03:41,450 --> 00:03:48,030
+أماكن أخرى في غزو في غزو للأنسجة فمن يمتلك انتشار
+
+48
+00:03:48,030 --> 00:03:51,310
+انتشار يمتلك المقدرة على أنه ينتقل من مكان إلى مكان
+
+49
+00:03:51,310 --> 00:03:56,740
+آخر السلمونيلا طيفية نشربها أو بناكلها مع الغذاء
+
+50
+00:03:56,740 --> 00:04:02,940
+السلمونيلا طيفية بسبب تايفويد فيفر السلمونيلا
+
+51
+00:04:02,940 --> 00:04:06,580
+طيفية بتتكاثر في الأمعاء لكن لا تكتفي بمكوثها في
+
+52
+00:04:06,580 --> 00:04:09,440
+الأمعاء تخترق جدار الأمعاء بتروح على ال lymph
+
+53
+00:04:09,440 --> 00:04:12,360
+node ومن ال lymph node بتروح على ال blood وبتصير
+
+54
+00:04:12,360 --> 00:04:16,660
+موجودة في الدم ومن الدم قد تنتقل إلى ما يسمى
+
+55
+00:04:16,660 --> 00:04:19,360
+reticuloendothelial system من ضمنها ال liver و
+
+56
+00:04:19,360 --> 00:04:24,160
+ال spleen و ال bone marrow ممكن وممكن تصل لل central
+
+57
+00:04:24,160 --> 00:04:28,760
+nervous system بتعمل هنا meningitis غزوًا ولا مش
+
+58
+00:04:28,760 --> 00:04:34,580
+غزوًا؟ غزوًا بينما ال Clostridium tetani ال بتاع
+
+59
+00:04:34,580 --> 00:04:43,590
+tetanus بتنغرز في مكان محدد وتبقى هناك ولا تستطيع
+
+60
+00:04:43,590 --> 00:04:46,590
+أنها تنتقل من مكان الإصابة إلى مكان آخر، بينما
+
+61
+00:04:46,590 --> 00:04:52,010
+الـtoxin تبعها هو اللي بينتقل، لكن هي localized،
+
+62
+00:04:52,010 --> 00:04:57,070
+الأولى systemic، انتشرت الأولى، انتشرت الثانية،
+
+63
+00:04:57,070 --> 00:05:01,770
+localized، وبالتالي الأولى invasive والثانية non
+
+64
+00:05:01,770 --> 00:05:06,380
+-invasive، non-invasive، كثير مهم المفهوم هذا وال
+
+65
+00:05:06,380 --> 00:05:10,920
+adherence وال invasion يعني معاني مختلفة لكن
+
+66
+00:05:10,920 --> 00:05:14,500
+كلهم بيصبوا في ال virulence وال virulence مقياس
+
+67
+00:05:14,500 --> 00:05:20,000
+للقدرة على إحداث المرض هو innocent المصطلح الثالث toxicity وبرضه
+
+68
+00:05:20,000 --> 00:05:24,940
+هذا مصطلح يصب في نفس الإطار بكتيريا تمتلك توكسين وبكتيريا
+
+69
+00:05:24,940 --> 00:05:30,620
+لا تمتلك توكسين خدوا بالكم من شغلة هناك بعض
+
+70
+00:05:30,620 --> 00:05:37,140
+الأمراض الأعراض كلها الناتجة عن المرض بتكون ناتجة
+
+71
+00:05:37,140 --> 00:05:40,700
+عن التوكسين نفسه زي التيتانوس اللي ذكرناه جاب شوية
+
+72
+00:05:40,700 --> 00:05:46,760
+البكتيريا اللي موجودة في القدم على سبيل المثال لكن
+
+73
+00:05:46,760 --> 00:05:50,660
+الأعراض متمثلة بشلل وشلل سباستيك بيرانسس
+
+74
+00:05:53,650 --> 00:05:58,210
+سببه مش وجود البكتيريا وإنما في التوكسين بمعنى
+
+75
+00:05:58,210 --> 00:06:01,690
+لو أخذنا البكتيريا وربيناها برة وأخذنا ال toxin
+
+76
+00:06:01,690 --> 00:06:05,390
+تبعها وحقنها في الإنسان يحدث نفس الأعراض تبع ال
+
+77
+00:06:05,390 --> 00:06:09,370
+tetanus إذا لما تكون البكتيريا toxigenic تختلف عن
+
+78
+00:06:09,370 --> 00:06:12,790
+ما يكون non-toxigenic بس بودأ أذكركم يا بنات
+
+79
+00:06:12,790 --> 00:06:16,430
+بالمفهوم اللي مرض على صناعة لما تكلمنا عن ال
+
+80
+00:06:16,430 --> 00:06:21,240
+bacteriophage لما تكلمنا عن ال lysogenic cycle لما
+
+81
+00:06:21,240 --> 00:06:24,960
+تكلمنا عن ال prophage  لداخل ولما تكلمنا عن ال
+
+82
+00:06:24,960 --> 00:06:29,140
+lysogenized cell وأن أن كثير من البكتيريا
+
+83
+00:06:29,140 --> 00:06:33,860
+التوكسوجينيك أصبحت توكسوجينيك لأن فيها prophage،
+
+84
+00:06:33,860 --> 00:06:37,300
+هذه هي اللي تبقى لنا ذاكرينها السيد روبرت كوخ 
+
+85
+00:06:37,300 --> 00:06:43,360
+للألمان وللكثيرين يعتبر هو أبو ال microbiology
+
+86
+00:06:43,360 --> 00:06:47,380
+الحديثة طبعًا طبعًا الفرنسيين بيقولوا لأ Louis
+
+87
+00:06:47,380 --> 00:06:52,760
+Pasteur لأنه هو فرنسي فالتاريخ يكتبه من .. من يكتبه 
+
+88
+00:06:52,760 --> 00:06:57,880
+Robert Koch من إنجازاته الكبيرة الصحيح أنه حاول
+
+89
+00:06:57,880 --> 00:07:03,380
+يعمل علاقة ما بين ال pathogen وما بين المرض قال
+
+90
+00:07:03,380 --> 00:07:07,420
+إذا أردنا أن نثبت أن هذا الميكروب هو المسبب لهذا
+
+91
+00:07:07,420 --> 00:07:12,470
+المرض فعلينا الإجراءات التالية الآن خدوا بالكم أن
+
+92
+00:07:12,470 --> 00:07:17,250
+الفرضية كانت صالحة في ذاك الزمن وفاتت أكلها بمعنى
+
+93
+00:07:17,250 --> 00:07:23,750
+أثبت من خلالها هو وغيره كثير من المسببات للأمراض
+
+94
+00:07:23,750 --> 00:07:26,890
+فال Vibrio cholera هي المسببة للكوليرا ال
+
+95
+00:07:26,890 --> 00:07:29,330
+Mycobacterium tuberculosis هي المسبب للسل 
+
+96
+00:07:29,330 --> 00:07:33,830
+ال Bacillus anthracis هي المسببة للأنتراكس خلينا
+
+97
+00:07:33,830 --> 00:07:36,810
+نتكلم مع بعض ونطلع على ال animation
+
+98
+00:07:39,760 --> 00:07:43,680
+الفرضية الأولى كانت بتقول أن الميكروب المسبب يجب
+
+99
+00:07:43,680 --> 00:07:48,140
+أن يكون موجودًا في الحيوان المصاب وليس موجودًا في
+
+100
+00:07:48,140 --> 00:07:53,180
+الحيوان السليم هذه أول خطوة في الفرضية الخطوة
+
+101
+00:07:53,180 --> 00:07:57,420
+الثانية إذا لازم نعزل الميكروب من الحيوان المصاب
+
+102
+00:07:57,420 --> 00:08:04,380
+بصورة نقية عشان نعجب بصورة نقية نحقنه في الحيوان
+
+103
+00:08:04,380 --> 00:08:09,230
+السليم يجب أن يظهر عليه نفس الأعراض يجب أن نقوم
+
+104
+00:08:09,230 --> 00:08:15,810
+بعزل الميكروب مرة أخرى، وصلت الصورة ولا لسه؟ إذا
+
+105
+00:08:15,810 --> 00:08:19,050
+ما وصلتش، نطلع على الخطوات، ياريت نركز على اللوحة
+
+106
+00:08:19,050 --> 00:08:24,910
+الآن، الـ microbe is present in each case of the
+
+107
+00:08:24,910 --> 00:08:28,810
+disease, but not in healthy animals، أعتقد أن
+
+108
+00:08:28,810 --> 00:08:32,530
+الصورة الآن اللي يبقى بنا بتوضح أن الفقر المصاب
+
+109
+00:08:32,530 --> 00:08:35,870
+فيه الميكروب، بينما ال healthy animal ما عندوش
+
+110
+00:08:35,870 --> 00:08:41,460
+الميكروب الخطوة الثانية إن نقوم بعزل ال microbe من
+
+111
+00:08:41,460 --> 00:08:45,320
+الفقر أو الحيوان اللي هو المصاب in pure culture
+
+112
+00:08:45,320 --> 00:08:48,420
+إذا
+
+113
+00:08:48,420 --> 00:08:51,640
+حقننا ال microbe اللي عزلناه من الأول في الثاني
+
+114
+00:08:51,640 --> 00:08:58,440
+السليم يجب أن يحدث عنه نفس اللي هو المرض ويجب أن
+
+115
+00:08:58,440 --> 00:09:02,800
+نقوم بعزل ال microbe مرة أخرى من ال experimentally
+
+116
+00:09:02,800 --> 00:09:07,190
+infected اللي هو animal طب أنا قاعد بقول إن الفرضية
+
+117
+00:09:07,190 --> 00:09:10,730
+في ذلك الزمن قعدت أكلها واليوم في عليها علامة
+
+118
+00:09:10,730 --> 00:09:14,870
+استفهام إيش علامة الاستفهام؟ الفرضية الأولى قالت إنه
+
+119
+00:09:14,870 --> 00:09:18,850
+يجب أن يكون في الحيوان المصاب ولا يزال في السليم
+
+120
+00:09:18,850 --> 00:09:23,190
+طب أنا بتعطيكم أمثلة كثيرة على مايكروبات موجودة في
+
+121
+00:09:23,190 --> 00:09:27,510
+المصاب وموجودة في السليم Staphylococcus aureus على
+
+122
+00:09:27,510 --> 00:09:30,710
+جلدنا موجودة وفي الفم تبعنا وعلى عينينا وعلى ..
+
+123
+00:09:30,710 --> 00:09:35,040
+يعني أماكن متنوعة من أجسامنا وفي نفس الوقت في ناس
+
+124
+00:09:35,040 --> 00:09:38,900
+مرضى عندهم دمامل وخراجات وحبوب اللي بيطلع منها
+
+125
+00:09:38,900 --> 00:09:44,480
+الصديد الأبيض Staphylococcus aureus إذا ليس بالضرورة
+
+126
+00:09:44,480 --> 00:09:47,980
+أن يكون الـ microbe في الـ .. في الـ .. يعني ممكن
+
+127
+00:09:47,980 --> 00:09:50,660
+يكون موجود في التانية في الـ .. لإن الـ infection
+
+128
+00:09:50,660 --> 00:09:54,000
+كانت interaction من ثلاث عوامل فاكرينهم؟ ثلاث
+
+129
+00:09:54,000 --> 00:09:58,440
+عوامل ذكرناهم الـ host immunity الـ type يبقى في
+
+130
+00:09:58,440 --> 00:10:01,700
+عامل مهم جدا كان اللي هو الـ host immunity ما أخدوش
+
+131
+00:10:01,700 --> 00:10:06,310
+بقى يعني اللي هو الاعتبار بدي أعطي مثال تاني بس
+
+132
+00:10:06,310 --> 00:10:10,090
+للاطمئنان في ميكروب مشهور جدا اسمه نيسيريا
+
+133
+00:10:10,090 --> 00:10:16,430
+مينينجيتايدس بتسبب التهاب السحايا المينينجايتس هذا
+
+134
+00:10:16,430 --> 00:10:20,530
+موجود كـ normal flora أو كـ normal microbiota في
+
+135
+00:10:20,530 --> 00:10:25,170
+أنوف أكثر من 15% من الناس اللي صحها يعني لو من 100
+
+136
+00:10:25,170 --> 00:10:30,530
+طالبة أخدنا مسحات من الأنف ممكن نازل من 15% منهم
+
+137
+00:10:30,530 --> 00:10:37,800
+للمهام نيسيريا مينينجيتايدس هذا يضحك اللي هو الـ band
+
+138
+00:10:37,800 --> 00:10:41,620
+الأول في فرضية كوخ إيش كمان في نقاط ضعف في فرضية
+
+139
+00:10:41,620 --> 00:10:47,320
+كوخ النقطة الثانية هي أنه أصر على أنه لازم نعزل
+
+140
+00:10:47,320 --> 00:10:52,060
+الميكروب بصورة نقية من الحيوان المصاب أو من حتى الـ
+
+141
+00:10:52,060 --> 00:10:55,700
+experimental الثاني طب هناك ميكروبات لحد الآن
+
+142
+00:10:55,700 --> 00:10:59,820
+ما قدرنا نميها في المختبر زي الـ mycobacterium leprae
+
+143
+00:10:59,820 --> 00:11:03,390
+اللي هي بسبب الجذام لحد الآن ما نميناها كثير من
+
+144
+00:11:03,390 --> 00:11:07,630
+الفيروسات لحد الآن ما قدرنا نميها في المختبر ومع 
+
+145
+00:11:07,630 --> 00:11:11,790
+ذلك أثبتنا أنهي المسؤولة بطرق أخرى غير اللي هو
+
+146
+00:11:11,790 --> 00:11:16,110
+فرضية كوخ إذا فرضية كوخ في ذاك الزمن كانت صالحة الآن
+
+147
+00:11:16,110 --> 00:11:18,750
+فيها عيبين أساسيين اللي هم العيب الأول اللي بكرناه
+
+148
+00:11:18,750 --> 00:11:21,710
+و الثاني اللي هو متعلق بعزل الـ micro
+
+149
+00:11:25,900 --> 00:11:31,700
+هذا ليس نقصا أو إنقاصا من حقوق Robert Koch بالعكس
+
+150
+00:11:31,700 --> 00:11:34,640
+يعني هو كان زي ما قلتلكوا إنه من العلماء اللي ..
+
+151
+00:11:34,640 --> 00:11:38,200
+يعني اللي .. اللي ساهموا وساهموا سهم عظيم في وضع الصورة
+
+152
+00:11:38,200 --> 00:11:41,920
+نفس الفكرة اللي أمامكم نفس الصورة اللي احنا
+
+153
+00:11:41,920 --> 00:11:46,610
+عرضناها في الـ animation اللوحة هذه التالت اللي
+
+154
+00:11:46,610 --> 00:11:49,350
+موجودة فيها تلت دوائر اللي هم الـ interaction ما
+
+155
+00:11:49,350 --> 00:11:54,010
+بين الـ immune system تبع الـ host و الجرعة و اللي
+
+156
+00:11:54,010 --> 00:11:57,610
+هو الـ strain اللي جونا الـ type و أنا ذكرت في المحاضرة
+
+157
+00:11:57,610 --> 00:12:03,470
+السابقة قلت شجرة نحتاج إلى 100 مليون microbe
+
+158
+00:12:03,470 --> 00:12:06,510
+تقريبا عشان نعمل infection عند الإنسان بينما الـ
+
+159
+00:12:06,510 --> 00:12:10,670
+virus اللي جونا نحتاج إلى مليون من واحد لمية يعني
+
+160
+00:12:10,670 --> 00:12:14,800
+الـ number قليلة مليون number عالية يعني لو بدنا
+
+161
+00:12:14,800 --> 00:12:17,880
+نحطهم في الميزان تبع الـ virulence بيقول مين is
+
+162
+00:12:17,880 --> 00:12:22,060
+more virulent الشغلة اللي هم عددها طبعا أقل اللي
+
+163
+00:12:22,060 --> 00:12:27,020
+هي عددها أقل كمان مرة ما ننساش التلات عوامل اللي
+
+164
+00:12:27,020 --> 00:12:30,020
+موجودين على اللوحة أمامنا اللي هو الـ immune system
+
+165
+00:12:30,020 --> 00:12:33,620
+الـ strength زي اللي هو الـ virulence و أنا أضفت لهم
+
+166
+00:12:33,620 --> 00:12:38,520
+بعد رابع المرة الماضية مين بيذكر البعد الرابع الـ
+
+167
+00:12:38,520 --> 00:12:43,370
+portal of entry مكان الدخول و ذكرتلكوا في المحاضرة
+
+168
+00:12:43,370 --> 00:12:47,430
+إنه مثال الـ .. الـ .. الـ bacillus anthracis لو دخل
+
+169
+00:12:47,430 --> 00:12:51,110
+عبر الجلد أو دخل عبر الـ lungs أو دخل عبر الـ
+
+170
+00:12:51,110 --> 00:12:54,630
+gastrointestinal الأرقام اختلفت تماما إذا الـ
+
+171
+00:12:54,630 --> 00:12:58,550
+portal of entry كمان كان بعد رابعا الصورة اللي
+
+172
+00:12:58,550 --> 00:13:03,810
+أمامكم بتتكلم عن bacterial virulence factors أنا لما
+
+173
+00:13:03,810 --> 00:13:07,710
+درستكوا الـ bacterial cell structure قلتلكوا
+
+174
+00:13:07,710 --> 00:13:13,470
+بناتنا الكلمات إنه لسنا نصدق إنه نحفظ بس التراكيب
+
+175
+00:13:13,470 --> 00:13:16,310
+إنه التراكيب هدس الـ wall هدس الـ membrane و أين
+
+176
+00:13:16,310 --> 00:13:21,470
+ما كان أقولت لأ احنا بدنا نفهم ما وراء التراكيب و
+
+177
+00:13:21,470 --> 00:13:26,030
+كنا كثير عن التراكيب أعطيكم مثال بسيط جدا و سهل كنا
+
+178
+00:13:26,030 --> 00:13:30,510
+نقول عن الـ flagella سميناها virulence factor لأنها بتهرب
+
+179
+00:13:30,510 --> 00:13:34,430
+من خطر و بتذهب إلى غذاء، بتذهب إلى غذاء من خلال
+
+180
+00:13:34,430 --> 00:13:38,710
+الحركة أو تهرب من خطر في حال إن تعرضت هذا الخطر،
+
+181
+00:13:38,710 --> 00:13:42,570
+هذا اعتبرنا احنا by an instinct لما تكلمنا على الـ
+
+182
+00:13:42,570 --> 00:13:46,190
+attachment تحدثنا أيضا على إن الـ attachment في
+
+183
+00:13:46,190 --> 00:13:50,770
+مكان محددة ضرورة من ضرورات البقاء و الـ
+
+184
+00:13:50,770 --> 00:13:53,830
+colonization تمام و بالتالي infection و بالتالي
+
+185
+00:13:53,830 --> 00:13:58,060
+disease يبقى كل التراكيب اللي درسناهم ما درسناهم
+
+186
+00:13:58,060 --> 00:14:04,320
+هكذا هكذا إن الآن موعدهم إن احنا نعطيهم حقهم في الـ
+
+187
+00:14:04,320 --> 00:14:09,780
+الـ الـ الـ virulence طيب
+
+188
+00:14:09,780 --> 00:14:13,980
+ما أعرفش الجملة ما أعرفش ليش مش واضحة في نقاط مش هتنزل
+
+189
+00:14:13,980 --> 00:14:18,660
+على اللوحة لكن بنقولكوا عنها regulation of
+
+190
+00:14:18,660 --> 00:14:23,350
+bacterial virulence factors كثير بيخطر على بال إن
+
+191
+00:14:23,350 --> 00:14:29,650
+احنا كبشر إن احنا كنا عند نفس المريض و تقريبا كنا
+
+192
+00:14:29,650 --> 00:14:33,330
+بنفس الدرجة من القرب من هذا المريض أنا أصيبت
+
+193
+00:14:33,330 --> 00:14:37,430
+بالمرض و زميل لم يصاب لماذا أنا أصبت و هو لم يصاب
+
+194
+00:14:37,430 --> 00:14:42,330
+أو العكس فتفسيراتنا بتقعد .. بنقعد .. بنحاول ..
+
+195
+00:14:42,330 --> 00:14:46,210
+نجتهد إن أنت كنت أقرب أكثر أو هو كان أقرب .. طب
+
+196
+00:14:46,210 --> 00:14:50,410
+هناك عوامل كثيرة احنا درسنا 3 عوامل قبل شوية منهم و
+
+197
+00:14:50,410 --> 00:14:55,060
+عملنا بواد رابع كنا منهم اللي هو الـ portal of
+
+198
+00:14:55,060 --> 00:15:00,560
+entry طيب الـ strain .. الـ strain عشان يعمل الـ
+
+199
+00:15:00,560 --> 00:15:06,620
+virulence في حاجة بيسموها إما triggering،
+
+200
+00:15:06,620 --> 00:15:11,280
+triggering يعني إطلاق أو inciting حث، inciting
+
+201
+00:15:11,280 --> 00:15:14,440
+factors، triggering factors أو trigger اللي هو ضغط
+
+202
+00:15:14,440 --> 00:15:18,240
+الزناد يعني، تضغط على الزناد ليش؟ تضغط على الزناد
+
+203
+00:15:18,240 --> 00:15:22,060
+to produce the virulence factor و أحد أهمها
+
+204
+00:15:22,060 --> 00:15:24,520
+شرحتلكم المرة الماضية أنا حاسماها quorum sensing
+
+205
+00:15:24,520 --> 00:15:28,020
+فاكرينها؟ quorum sensing لما قلنا إنه عدد
+
+206
+00:15:28,020 --> 00:15:33,080
+البكتيريا، البكتيريا عندها المقدرة تشعر و إذا
+
+207
+00:15:33,080 --> 00:15:36,760
+استشعرت إن عددها صار مؤهل أن تنتج توكسين تنتج
+
+208
+00:15:36,760 --> 00:15:40,800
+التوكسين هو الـ virulence مش هو virulence factor توكسين
+
+209
+00:15:40,800 --> 00:15:44,280
+طب إيش العوامل الأخرى اللي ممكن تأثر على إنتاج الـ
+
+210
+00:15:44,280 --> 00:15:47,340
+virulence factors؟ أنا بقولوا أحدها الـ
+
+211
+00:15:47,340 --> 00:15:54,640
+temperature، الـ pH، ممكن الـ osmolarity، ممكن الـ
+
+212
+00:15:54,640 --> 00:16:01,060
+iron، الـ iron يعني الحديد بعض الـ ions المحددة إذا
+
+213
+00:16:01,060 --> 00:16:05,620
+وجدت أو لم توجد كلها ممكن تساهم في switch on و
+
+214
+00:16:05,620 --> 00:16:10,780
+switch off يبقى
+
+215
+00:16:10,780 --> 00:16:16,140
+الآن لما بنيجي نفسر لماذا فلان أصيب و فلان لم يصاب
+
+216
+00:16:16,140 --> 00:16:19,800
+الأمور ازدادت تعقيدا الأمور ازدادت تعقيدا كيف
+
+217
+00:16:19,800 --> 00:16:23,980
+ازدادت تعقيدا احنا كنا متكلمين على تلات عوامل host
+
+218
+00:16:23,980 --> 00:16:30,500
+immunity و strain و إيش كمان؟ و الـ dose طبعا طيب احنا
+
+219
+00:16:30,500 --> 00:16:34,200
+اتعرضنا لنفس الـ strain و اتعرضنا لنفس الـ dose على
+
+220
+00:16:34,200 --> 00:16:36,440
+سبيل المثال مين ضايل الآن التالت؟
+
+221
+00:16:40,870 --> 00:16:44,970
+طيب احنا الـ immunity تبعتنا كانت determined عامل
+
+222
+00:16:44,970 --> 00:16:48,830
+محدد عامل محدد طب في كمان عامل محدد احنا إثنين
+
+223
+00:16:48,830 --> 00:16:52,930
+افترقنا عن بعض احنا إثنين افترقنا عن بعض أنا تعرضت
+
+224
+00:16:52,930 --> 00:16:57,950
+لظروف و أنت تعرضت لظروف أخرى قد تساهم في switching
+
+225
+00:16:57,950 --> 00:17:01,470
+on أو switching off لمين؟ لبعض الـ virulence factors
+
+226
+00:17:01,470 --> 00:17:05,930
+إذا خلينا نقول من خلال معرفتنا بأن في regulation
+
+227
+00:17:07,100 --> 00:17:10,120
+لإنتاج يبقى الـ virulence factor مش دائما هو نفس
+
+228
+00:17:10,120 --> 00:17:15,340
+الإنتاج بيعتمد على ظروف معينة بيئية و غذائية يبقى
+
+229
+00:17:15,340 --> 00:17:18,500
+معنا الحكي بنقدر نفسر لماذا هناك اختلافات في نسبة
+
+230
+00:17:18,500 --> 00:17:22,320
+الإصابة بين مجموعة من البشر تعرضوا لنفس اللي إيه؟
+
+231
+00:17:22,320 --> 00:17:26,280
+نفس الظروف أول
+
+232
+00:17:26,280 --> 00:17:31,940
+أول نقطة بنركز عليها اليوم في الـ virulence factor
+
+233
+00:17:31,940 --> 00:17:34,860
+اللي هو الـ adherence factor اللي نقول عنهم
+
+234
+00:17:34,860 --> 00:17:40,220
+adherence, adhesion أو attachment أتولد صغير كثير
+
+235
+00:17:40,220 --> 00:17:44,600
+خالص آه يعني غير مرئي حتى بالنسبة لي أنا هتطلع ألبس
+
+236
+00:17:44,600 --> 00:17:52,250
+المضادة بدأ أقرأ بس بالأول أسماء للمركبات اللي
+
+237
+00:17:52,250 --> 00:17:56,410
+كلها اعتبرناها adherence factors adhesin,
+
+238
+00:17:57,010 --> 00:18:02,710
+receptor, lectin, ligand, mucus, fimbria, common
+
+239
+00:18:02,710 --> 00:18:08,050
+pili, sex pili، اللي هي sex pili يعني، pili جمع،
+
+240
+00:18:08,050 --> 00:18:12,710
+pilus اللي هي singular، type 1 fimbria، type 4
+
+241
+00:18:12,710 --> 00:18:18,950
+pili, S layer الـ S Layer مرة عليكم Slime Layer و S
+
+242
+00:18:18,950 --> 00:18:22,010
+Layer و Capsule و Micro Capsule الجليكوكاليكس
+
+243
+00:18:22,010 --> 00:18:26,210
+الاسم العام لكلهم Capsule Lipopolysaccharide
+
+244
+00:18:26,210 --> 00:18:33,410
+Teichoic Acid and Lipoteichoic Acid كل ما بكرناه هي
+
+245
+00:18:33,410 --> 00:18:37,510
+تراكيب موجودة في بعض أنواع من البكتيريا و غير
+
+246
+00:18:37,510 --> 00:18:43,410
+موجودة في بعض أنواع أخرى مع ده رقم إثنين اليمين الـ
+
+247
+00:18:43,410 --> 00:18:49,570
+receptor الـ receptor بنطلقه على التركيب اللي موجود
+
+248
+00:18:49,570 --> 00:18:52,270
+في خلايا الـ eukaryotes اللي هم الـ host اللي هم
+
+249
+00:18:52,270 --> 00:18:56,030
+أحلى تمام اللي ممكن يرتبط فيها واحد من هذه
+
+250
+00:18:56,030 --> 00:19:01,390
+المركبات اللي هو البكتيريا الصورة بمعنى آخر إنه
+
+251
+00:19:01,390 --> 00:19:06,130
+مثلا الأدهيسين بدهو receptor الليكتين بدهو receptor
+
+252
+00:19:06,130 --> 00:19:09,170
+الليجان بدهو receptor يبقى ال receptor هو المركب
+
+253
+00:19:09,170 --> 00:19:14,790
+المستقبل طيب في نقطة كتير مهمة أذكركم و أرجع بيكم 
+
+254
+00:19:14,790 --> 00:19:20,250
+بالبكتيروفاج البكتيروفاج عمل infection لمن؟
+
+255
+00:19:20,250 --> 00:19:25,090
+للبكتيريا لم يستطيع أن يعمل infection لا للنباتات
+
+256
+00:19:25,090 --> 00:19:31,730
+ولا للحيوانات تمام؟ الآن بدنا .. برضه بدنا نعكس
+
+257
+00:19:31,730 --> 00:19:36,350
+الموضوع الفيروس اللي بيصيبنا احنا تمام؟ احنا
+
+258
+00:19:36,350 --> 00:19:39,670
+بيسموه animal viruses أو human viruses تمام؟ ما
+
+259
+00:19:39,670 --> 00:19:43,270
+بتصيب النباتات؟ ما بتصيب النباتات؟ لأن مافيش الـ
+
+260
+00:19:43,270 --> 00:19:49,590
+هي receptors للنباتات و هذا يسمى host specificity
+
+261
+00:19:49,590 --> 00:19:56,070
+يعني في نوع من التخصص ما بين ال microbe المعني و
+
+262
+00:19:56,070 --> 00:20:01,930
+ما بين ال host أو بالظبط ال host يعني بيصيب البط و
+
+263
+00:20:01,930 --> 00:20:05,730
+بيصيب الحمام لكن ما بيصيبش الخنازير على سبيل المثال
+
+264
+00:20:05,730 --> 00:20:10,090
+أو بيصيب الخنازير و الخيل و لكن ما بيصيبش
+
+265
+00:20:10,090 --> 00:20:14,990
+الحمام مؤشر عليا على أنه لا يحتوي ال receptor في
+
+266
+00:20:14,990 --> 00:20:17,290
+الحيوانات وهذا الكلام على فكرة بنشوفه في الـ
+
+267
+00:20:17,290 --> 00:20:21,630
+influenza بنلاقي ال influenza بعض .. بعض السلالات
+
+268
+00:20:21,630 --> 00:20:26,930
+منها بتصيب الطيور و بعض السلالات بتصيب مثلا الخيل
+
+269
+00:20:26,930 --> 00:20:32,270
+البعض بتصيب الخنازير و البعض بتصيب خد بالك قد يحدث
+
+270
+00:20:32,270 --> 00:20:37,400
+أن يكون في عامل مشترك مثلا اللي أجي فيروس influenza
+
+271
+00:20:37,400 --> 00:20:42,260
+بيصيب الخيل و الخنزير و واحد بيصيب الإنسان و
+
+272
+00:20:42,260 --> 00:20:45,440
+الخنزير لكن ما بيصيبش الإنسان و الخيل في نفس الوقت،
+
+273
+00:20:45,440 --> 00:20:49,160
+مين العامل المشترك اللي أهان؟ الخنزير أكرمكم الله،
+
+274
+00:20:49,160 --> 00:20:53,470
+و هذا اللي بيحدث عندها تغيير تفراد إنه سلالتين
+
+275
+00:20:53,470 --> 00:20:56,790
+واحدة من الإنسان و واحدة من الخيل بيعملوا الـ co
+
+276
+00:20:56,790 --> 00:21:00,630
+-infection في نفس اللحظة في الخنزير فقد يحدث تبادل
+
+277
+00:21:00,630 --> 00:21:05,610
+للجينات باسمها gene reassortment هدرسها لاحقا و الـ
+
+278
+00:21:05,610 --> 00:21:09,030
+reassortment هذي عبارة عن استبدال قطع من المادة
+
+279
+00:21:09,030 --> 00:21:13,190
+الوراثية من كل من الـ virusين و بيطلعنا سلالة
+
+280
+00:21:13,190 --> 00:21:19,690
+جديدة سلالة جديدة يبقى معنا الحكي أنه بنفهم أنه
+
+281
+00:21:19,690 --> 00:21:25,610
+عملية ال .. ال .. ال .. ال specificity تبع الـ
+
+282
+00:21:25,610 --> 00:21:29,210
+receptor مرتبطة .. أو أفرض ال .. ال .. ال .. الـ
+
+283
+00:21:29,210 --> 00:21:33,090
+infection مرتبطة بوجود receptor للعوامل تبع الـ
+
+284
+00:21:33,090 --> 00:21:36,310
+attachment تبع ال bacteria أو اللي هو الفيروسة
+
+285
+00:21:36,310 --> 00:21:40,370
+و احنا بنقولكم كمان مرة دائما و أبدا أول خطوة في
+
+286
+00:21:40,370 --> 00:21:42,670
+ال infection غالبا هي ال attachment
+
+287
+00:21:45,700 --> 00:21:48,780
+أنا قلت عن مين؟ عن ال species أو ال host
+
+288
+00:21:48,780 --> 00:21:53,220
+specificity، صح؟ طب داخل ال host الواحد، احنا زي
+
+289
+00:21:53,220 --> 00:21:57,980
+ما مثلا إنسان بشر، تراكيب الخلايا تبعته و الأنسجة
+
+290
+00:21:57,980 --> 00:22:01,280
+و الأعضاء مختلفة تماما، ال cardiac muscle غير عن
+
+291
+00:22:01,280 --> 00:22:03,280
+ال intestinal، ال epithelium اللي موجود في
+
+292
+00:22:03,280 --> 00:22:06,420
+الانتستين غير عن ال epithelium اللي موجود في الـ
+
+293
+00:22:06,420 --> 00:22:11,670
+lungs، فهل هناك هل يكون هناك مجتمع عالمي على كل
+
+294
+00:22:11,670 --> 00:22:16,230
+الأنسجة؟ يمكن لأ و يمكن لأن في بعض المركبات لازم
+
+295
+00:22:16,230 --> 00:22:21,310
+تكون مشتركة بين كل الخلايا مثلًا مجتمع لـ الجلوكوز
+
+296
+00:22:21,310 --> 00:22:22,070
+على سبيل المثال
+
+297
+00:22:25,800 --> 00:22:29,080
+إذا ما كانش على كل الأنثى لهذا الريسبتور اللي
+
+298
+00:22:29,080 --> 00:22:32,280
+بتستخدمه البكتيريا في الإرتباط فمع ذلك الحكي إن هذا
+
+299
+00:22:32,280 --> 00:22:35,360
+الريسبتور هو اللي بيحدد ال organ أو ال tissue
+
+300
+00:22:35,360 --> 00:22:39,320
+tropism أو ال specificity كلمة tropism يعني الـ
+
+301
+00:22:39,320 --> 00:22:43,180
+affinity affinity يعني القابلية للإرتباط، تمام؟
+
+302
+00:22:43,180 --> 00:22:46,340
+ففي عندي شغلتين أنثى عندنا من ال adherence واحدة
+
+303
+00:22:46,340 --> 00:22:51,900
+متعلقة بال species specificity و تانية من dual
+
+304
+00:22:51,900 --> 00:22:55,000
+species اللي هو tissue tropism
+
+305
+00:22:58,370 --> 00:23:01,910
+طيب الجدول هذا اللي قدامكم بنطلع عليه كويس لأن هو
+
+306
+00:23:01,910 --> 00:23:08,130
+اللي بوضح الفكرة تماما بتقرأ أسماء البكتيريا اللي
+
+307
+00:23:08,130 --> 00:23:15,370
+في أقصى اليسار و بعدين تطلع على العمود الأخير عشان
+
+308
+00:23:15,370 --> 00:23:20,430
+توضح نقطة Streptococcus pyogenes هذه رفيقة الدرب
+
+309
+00:23:20,430 --> 00:23:25,270
+تبعكم تذكروا دائما أنه يوم ما تنزل اللوز تصير
+
+310
+00:23:25,270 --> 00:23:28,710
+عندك اللوز يعني التهاب اللوز هيعرف أنه هذي
+
+311
+00:23:28,710 --> 00:23:32,990
+Streptococcus pyogenes Streptococcus mutans و الـ
+
+312
+00:23:32,990 --> 00:23:36,590
+Streptococcus salivarius هدول تلاتة موجودين في
+
+313
+00:23:36,590 --> 00:23:40,790
+اللعاب في الفم ك normal flora ك normal
+
+314
+00:23:40,790 --> 00:23:46,970
+flora لكن ممكن أحداهم اللي هي الأولى ال mutans هي
+
+315
+00:23:46,970 --> 00:23:49,370
+تعمل dental caries تسوس الأسنان
+
+316
+00:23:52,850 --> 00:23:56,390
+يعني قاصد أن نتكلم على الأسماء بسرعة عشان احنا
+
+317
+00:23:56,390 --> 00:24:00,230
+نشوف الأمراض و نشوف أماكن الأمراض هذه و إيش علاقة
+
+318
+00:24:00,230 --> 00:24:03,930
+الأدوات بين الأمراض بالظبط هذي Streptococcus pneumoniae
+
+319
+00:24:03,930 --> 00:24:08,310
+pneumonia؟ أين موجودة؟ pneumonia .. pneumonia .. في
+
+320
+00:24:08,310 --> 00:24:11,970
+الرئة ثاني، تمام Streptococcus aureus؟ هذه في كل
+
+321
+00:24:11,970 --> 00:24:16,950
+مكان، في كل مكان، تمام؟ Neisseria gonorrhoeae؟ مرض جنسي
+
+322
+00:24:16,950 --> 00:24:21,850
+في الـ Urogenital، في الـ Urogenital tract طيب اللوحة
+
+323
+00:24:21,850 --> 00:24:24,870
+اللي بعدها Enterotoxigenic Escherichia coli في الـ
+
+324
+00:24:24,870 --> 00:24:28,430
+intestine اللي بتعمل ديارية ال Uropathogenic
+
+325
+00:24:28,430 --> 00:24:31,510
+Escherichia coli ممكن أنا مرتين لغرضين لكن واحدة
+
+326
+00:24:31,510 --> 00:24:34,170
+بتعمل urethritis واحدة بتعمل pyelonephritis وين الـ
+
+327
+00:24:34,170 --> 00:24:37,610
+pyelonephritis؟ في الكلية الكلية بتنزل ال urine
+
+328
+00:24:37,610 --> 00:24:41,710
+تمام؟ فيه باستمرار washing ال urethritis الـ
+
+329
+00:24:41,710 --> 00:24:45,030
+urethra اللي هي العضو الأخير في ال urinary tract
+
+330
+00:24:45,030 --> 00:24:49,880
+اللي بعد اللي هو ال blood و برضه الأمور عبره مين؟ الـ
+
+331
+00:24:49,880 --> 00:24:53,940
+bladder و ال bladder بيعمل عملية washing تمام؟ الـ
+
+332
+00:24:53,940 --> 00:24:57,720
+Whooping cough في ال .. في ال lungs تمام؟ في
+
+333
+00:24:57,720 --> 00:25:00,220
+التراكيا اللي هي السعال الديكي السعال الديكي
+
+334
+00:25:00,220 --> 00:25:03,700
+اللي بسببها Bordatella و Pertussis Vibrio cholerae
+
+335
+00:25:03,700 --> 00:25:08,000
+diarrhoea Treponema pallidum syphilis مرض جنسي
+
+336
+00:25:08,000 --> 00:25:12,840
+Mycoplasma pneumoniae في ال lungs Chlamydia
+
+337
+00:25:12,840 --> 00:25:19,320
+conjunctivitis التهابات العين أو urethritis
+
+338
+00:25:19,320 --> 00:25:22,560
+زي ال extension كلها الأولانية، طب تعالوا نطلع على
+
+339
+00:25:22,560 --> 00:25:27,260
+الأماكن اللي احنا ذكرناها sore throat احنا بكونا
+
+340
+00:25:27,260 --> 00:25:33,760
+نلتهب لكن ما بنصومش، بمعنى بناكل و بنشرب، طب البلع
+
+341
+00:25:33,760 --> 00:25:37,500
+مش عملية ضغط على ال tonsils، هناك مش عارفين مش
+
+342
+00:25:37,500 --> 00:25:42,060
+عارفين القلم شديد مش الميكروبات المفروض إذا ما عندناش
+
+343
+00:25:42,060 --> 00:25:45,000
+المقدرة على ال attachment و موجودة على ال .. تنزل
+
+344
+00:25:45,000 --> 00:25:48,800
+فيها تروح مع الأكل و تنزل على .. على المعدة و تموت
+
+345
+00:25:48,800 --> 00:25:52,400
+في المعدة لكن واقع الأمر هناك أنواع من الـ
+
+346
+00:25:52,400 --> 00:25:55,380
+tonsillitis لما بنطلّع عليها بنفتح الفم و بنشوفها
+
+347
+00:25:55,380 --> 00:25:59,920
+بنلاقي بقع بيضاء على ال tonsil نفسها عارفين شو
+
+348
+00:25:59,920 --> 00:26:03,280
+البقع البيضاء هذي؟ مستعمرات Streptococcus pyogenes
+
+349
+00:26:03,280 --> 00:26:06,560
+مستعمرات دلالة على أن البكتيريا قادرة على الـ
+
+350
+00:26:06,560 --> 00:26:11,240
+attach بشكل قوي جدا في هذا المكان طب نيجي للـ
+
+351
+00:26:11,240 --> 00:26:16,400
+pneumonia احنا أول ما نعملها لما بصيرنا
+
+352
+00:26:16,400 --> 00:26:20,220
+pneumonia بصير ننكحم  ظبط؟ طب و الكحة عبارة على
+
+353
+00:26:20,220 --> 00:26:24,660
+فعلين عن إيش؟ عن explosion من الهواء بيضغط ..
+
+354
+00:26:24,660 --> 00:26:27,740
+بيضغط .. بيضغط .. بيضغط فيه أمبوبرا فيها
+
+355
+00:26:27,740 --> 00:26:31,380
+الـ trachea، يعني المفروض أن أي بكتيريا مع ال .. مع
+
+356
+00:26:31,380 --> 00:26:36,920
+القدائف إيش يحصل فيها، تطلع برة، ومع ذلك عند
+
+357
+00:26:36,920 --> 00:26:40,100
+المقدرة على attachment بتبقى ملازقة جنوبها طب
+
+358
+00:26:40,100 --> 00:26:43,500
+عارفين الـ trachea فيها ما يسمى بالـ Cilia Reactions
+
+359
+00:26:43,500 --> 00:26:47,900
+Cilia Reactions اللي هي حركة ال cilia الأهداب حركة
+
+360
+00:26:47,900 --> 00:26:51,880
+ال cilia هذه موجودة باستمرار 24 ساعة شغالة و
+
+361
+00:26:51,880 --> 00:26:56,180
+بيسموها المصعد إيش هي المصعد؟ آسانسير بينزل ..
+
+362
+00:26:56,180 --> 00:27:00,080
+بيجيب من تحت بيطلع وين؟ بيطلع برة إيش بيطلع؟ بيطلع
+
+363
+00:27:00,080 --> 00:27:05,320
+الـ mucus، المخاط جسمنا أو ال lungs بتنتج mucus
+
+364
+00:27:05,320 --> 00:27:07,900
+عشان تعمل treatment للغبار و ال micro organism
+
+365
+00:27:09,630 --> 00:27:16,710
+فباستمرار ال lungs بتنتج mucus بيرتبط بالغبار
+
+366
+00:27:16,710 --> 00:27:20,430
+و بال micro organism عشان ما يستقرش ال micro
+
+367
+00:27:20,430 --> 00:27:24,650
+organism بنطلع برة و بنتخلص منه عبر الكحة الخفيفة
+
+368
+00:27:24,650 --> 00:27:29,630
+في معظم الأحيان تخيلوا أن ال cilia اللي بتتحرك هذه
+
+369
+00:27:29,630 --> 00:27:32,910
+الحركة المنتظمة فجأة واحد قد مسك ال cilia هيك
+
+370
+00:27:39,870 --> 00:27:43,610
+أنه في بكتيريا بتجري تعمل attachment على ال cilia،
+
+371
+00:27:43,610 --> 00:27:48,630
+تمام؟ فبيصير في وزن و ارتباط عدم انتظام في الحركة
+
+372
+00:27:48,630 --> 00:27:51,870
+فبيصير في عندي accumulation في ال Pertussis في ال ..
+
+373
+00:27:51,870 --> 00:27:55,910
+اللي السعال الديكي accumulation للبكتيريا و الـ
+
+374
+00:27:55,910 --> 00:28:00,150
+mucus في منطقة محددة فبيصير في عندي عملية السعال
+
+375
+00:28:00,150 --> 00:28:03,530
+اللي هو اللي مستمر لفترة طويلة اللي بيقولنا عنه مش 
+
+376
+00:28:03,530 --> 00:28:07,660
+بسعادة طيب الدوسنتاريا مش هنقول نشوفنا
+
+377
+00:28:07,660 --> 00:28:11,280
+إنترولوكتكسوجينك إشريشيا كلها و نشوفنا الفيبرو كلها
+
+378
+00:28:11,280 --> 00:28:14,960
+بتعمل إسهال و الإسهال عبارة عن برضه running water
+
+379
+00:28:14,960 --> 00:28:18,240
+running water مياه جارية مش مفروض تعمل flushing
+
+380
+00:28:18,240 --> 00:28:24,340
+للَبَكتيريا و مع ذلك البكتيريا تبقى هناك تتجمد
+
+381
+00:28:24,340 --> 00:28:27,640
+تعالوا نطلعوا على اللي بتسبب urinary tract أو 
+
+382
+00:28:27,640 --> 00:28:30,720
+genitourinary tract طب ما ال urine يقولنا احنا
+
+383
+00:28:30,720 --> 00:28:35,010
+برضه عملية تنظيف ومع ذلك البكتيريا تبقى هناك إذا
+
+384
+00:28:35,010 --> 00:28:39,470
+بدنا نطلع دعاية من الاحترام والتقدير على المركبات
+
+385
+00:28:39,470 --> 00:28:43,550
+اللي بتخلي البكتيريا تبقى في هذه الأماكن لأنها
+
+386
+00:28:43,550 --> 00:28:49,710
+المسؤولة طيب أعطيكم مثال على بي لاي اللي موجودة في 
+
+387
+00:28:49,710 --> 00:28:57,990
+نيصوريا جونورية وراحت آخر واحدة في القائمة الآن
+
+388
+00:28:57,990 --> 00:29:04,040
+هذه البكتيريا هذه البكتيريا رقم واحد فيها virulence
+
+389
+00:29:04,040 --> 00:29:08,260
+factor هو الـ P-Ly هو الـ P-Ly طب تخيلوا جسم الإنسان
+
+390
+00:29:08,260 --> 00:29:11,340
+هو ذكي برضه الـ immune system تبقى على ذكي بيروح
+
+391
+00:29:11,340 --> 00:29:16,500
+بيكوّن antibody لمين؟ للـ P-Ly عشان ترجعله وعارف
+
+392
+00:29:16,500 --> 00:29:20,400
+حالها بدون P-Ly عارف حالها بدون P-Ly غير قادرة على
+
+393
+00:29:20,400 --> 00:29:24,060
+أنها تبقى في هذا المكان فبتروح تعمل mutation في
+
+394
+00:29:24,060 --> 00:29:27,520
+مين؟ في الـ P-Ly في الروتينة وبالتالي ترجع مرة
+
+395
+00:29:27,520 --> 00:29:32,780
+ثانية توصل الصورة يعني في الـ attachment ثبت بالدليل
+
+396
+00:29:32,780 --> 00:29:36,980
+القاطع من اللوحة اللي قدامنا على الرغم من اختلاف
+
+397
+00:29:36,980 --> 00:29:41,200
+المركبات المستخدمة في الـ attachment إلا أنه الـ
+
+398
+00:29:41,200 --> 00:29:45,600
+attachment كغراب هي عامل مهم جدا في عاملته اللي هو
+
+399
+00:29:45,600 --> 00:29:49,700
+الـ path Genesis وبالتالي هي viral as fuck وانا
+
+400
+00:29:49,700 --> 00:29:56,610
+اتفقنا أنه أي عامل بيأثر إيجابا على الـ pathogenesis
+
+401
+00:29:56,610 --> 00:30:03,810
+تبع البكتيريا تمام هو violence factor هذه الفكرة
+
+402
+00:30:03,810 --> 00:30:08,110
+العامة على ال addition أنه البكتيريا من خلال
+
+403
+00:30:08,110 --> 00:30:11,530
+الأدهسين اللي أين من الأشياء الأخرى اللي ذكرناها
+
+404
+00:30:11,530 --> 00:30:15,250
+اسمه ادهسين بيرتبط على ال receptor اللي مولّد في الـ
+
+405
+00:30:15,250 --> 00:30:19,240
+epithelial cell سبعة أنسجة الإنسان مثل الـ
+
+406
+00:30:19,240 --> 00:30:22,400
+Streptococcus biogenes كانت حاسمة protein F أو F
+
+407
+00:30:22,400 --> 00:30:26,240
+protein وبيرتبط مع الفيبرونكتين اللي موجود على بعض
+
+408
+00:30:26,240 --> 00:30:29,300
+الـ epithelial cells و هذا ال binding قولنا binding
+
+409
+00:30:29,300 --> 00:30:33,960
+قوي و من خلاله البكتيريا تبقى هناك على الرغم من
+
+410
+00:30:33,960 --> 00:30:41,520
+أنه في forces بتحاول أن تخلص منك الفيمبريا أنا
+
+411
+00:30:41,520 --> 00:30:45,820
+ممكن في ال structure حاولت أني أمر مرور سريع على 
+
+412
+00:30:45,820 --> 00:30:49,220
+وظيفتين رئيسيتين في الفيمبريا أو البيلاي واحنا
+
+413
+00:30:49,220 --> 00:30:51,660
+كنا الفيمبريا و البيلاي كانوا اسمين بيستخدموه
+
+414
+00:30:51,660 --> 00:30:55,400
+interchangeably بمعناه أنهم بي .. مرات بيميزوش
+
+415
+00:30:55,400 --> 00:30:59,380
+بينهم أساسا لكن خصصوا الـ .. البيلاي الـ يسموها
+
+416
+00:30:59,380 --> 00:31:02,520
+الـ six-pili و التانية قالوا عنها attachment fimbria
+
+417
+00:31:02,520 --> 00:31:06,180
+لكن بواقع الأمر لحد الآن كثير من الـ textbook
+
+418
+00:31:06,180 --> 00:31:10,200
+بتتكلم عنهم كانهم تركيب واحد اللي أنا بدأ أقوله
+
+419
+00:31:10,200 --> 00:31:13,760
+اليوم مختلف عن اللي قلته في السابق مش لأنه
+
+420
+00:31:13,760 --> 00:31:17,940
+حكيت لكم علامات خطأ لأ ما حبناش نعمل confusion في
+
+421
+00:31:17,940 --> 00:31:21,660
+البداية بس الآن لازم نقول لكم الحقيقة المرة أنه
+
+422
+00:31:21,660 --> 00:31:26,780
+البيلاي و الفيمبريا ليست بهذه البساطة التي
+
+423
+00:31:26,780 --> 00:31:32,040
+درسناها يعني بمعنى آخر ممكن تلاقوا textbook كامل
+
+424
+00:31:32,040 --> 00:31:39,550
+عن البيلاي و الفيمبريا بيشمل مؤتمرات مظلمة فقط
+
+425
+00:31:39,550 --> 00:31:43,990
+لدراسة البيلاي و الفيمبريا طبعا للي أكيد لهميتها وأنا
+
+426
+00:31:43,990 --> 00:31:47,810
+قاعد بتكلم إلي ربع ساعة عن الـ attachment و أهمية
+
+427
+00:31:47,810 --> 00:31:51,070
+الـ attachment فيه مش بس في الـ .. في الـ .. في الـ
+
+428
+00:31:51,070 --> 00:31:54,630
+pathogenesis تخيلوا في المستشفيات البكتيريا اللي 
+
+429
+00:31:54,630 --> 00:31:58,170
+عندها قدرة على الـ attachment مع الأسطح قد تكون
+
+430
+00:31:58,170 --> 00:32:03,230
+يسمى biofilms إيش يعني bio؟ films طبقات بيولوجية
+
+431
+00:32:04,040 --> 00:32:07,140
+طيب أقول لكم إيش يعني biofilm عشان تقدروا الموضوع
+
+432
+00:32:07,140 --> 00:32:09,360
+الـ attachment طبعا الـ biofilm كلكم شفتوه في
+
+433
+00:32:09,360 --> 00:32:13,780
+الدار لما عملت مخلة زيتون و عفن طلع فوق هذا
+
+434
+00:32:13,780 --> 00:32:17,700
+الأبيض و المخلة الخيار هذا اسمه biofilm ليش
+
+435
+00:32:17,700 --> 00:32:22,000
+البكتيريا طاع فيه على سطر هذه بكتيريا لأن هي عندها
+
+436
+00:32:22,000 --> 00:32:25,020
+المقدرة على الـ attachment بينها و بين بعضها لكن
+
+437
+00:32:25,020 --> 00:32:27,780
+هناك بكتيريا عندها المقدرة على الـ attachment بينها
+
+438
+00:32:27,780 --> 00:32:32,640
+و بين الأسطح تخيلوا يا بنات مكينة غذاء بنقطع فيها
+
+439
+00:32:32,640 --> 00:32:37,990
+غذاء بقايا بيضل شوية بقايا فبتجي البكتيريا بتنمو
+
+440
+00:32:37,990 --> 00:32:40,950
+لما تنمو بتروح اللي هي ملازجة في الماكينة نفسها
+
+441
+00:32:40,950 --> 00:32:45,230
+أيه طبعا بنغسلها بنرشها بالمي احنا في أماكن يصعب
+
+442
+00:32:45,230 --> 00:32:49,970
+عينا الوصول الفرشاية فبتكون هناك هذه البكتيريا إذا
+
+443
+00:32:49,970 --> 00:32:54,180
+.. إذا من النوع اللي بيكون biofilm حتى لو حطينا
+
+444
+00:32:54,180 --> 00:32:58,620
+عليها كلور خدوا بالكم البكتيريا الـ behavior تبعها
+
+445
+00:32:58,620 --> 00:33:03,980
+لما بتكون single cells خلايا منفصلة بيختلف تماما
+
+446
+00:33:03,980 --> 00:33:08,860
+عن ما لو كانت biofilm يعني لو كنا نقتلها بتركيز 3%
+
+447
+00:33:08,860 --> 00:33:14,700
+كلور تمام بدنا 6% كلور للـ biofilm أو 10% كلورين للـ
+
+448
+00:33:14,700 --> 00:33:18,500
+biofilm الـ behavior تبع الـ biofilm بيختلف أحد
+
+449
+00:33:18,500 --> 00:33:22,850
+مظاهر الاختلاف هو its ability to resist resist
+
+450
+00:33:22,850 --> 00:33:25,930
+what? different methods of disinfection and
+
+451
+00:33:25,930 --> 00:33:29,370
+sterilization طب الكلام اللي بحكيه أنا هذا ما هو
+
+452
+00:33:29,370 --> 00:33:32,330
+نفسه بينطبق على المستشفى لما أنا أركب canula
+
+453
+00:33:32,330 --> 00:33:36,370
+للمريض الـ canula وإن كانت مصمومة من الـ plastic
+
+454
+00:33:36,370 --> 00:33:40,210
+تمام و الـ plastic ناعم و هي معقمة و تمام تمام إلا
+
+455
+00:33:40,210 --> 00:33:45,850
+أنه بالنسبة للبكتيريا هي جسم خشن جدا بالنسبة لحجم
+
+456
+00:33:45,850 --> 00:33:50,590
+البكتيريا خشن جدا و تستطيع أن تبقى ملتصقا فيه
+
+457
+00:33:51,680 --> 00:33:54,820
+تعرفون أنتم مرضى الشلل الرباعي أو النصف اللي
+
+458
+00:33:54,820 --> 00:33:59,540
+بيصير أنه شلل بعد نتيجة حادث أو كده طبعا بيصيروا
+
+459
+00:33:59,540 --> 00:34:03,100
+يفقدوا السيطرة على التبول فبيركبوا لهم قسطرة بولية
+
+460
+00:34:03,100 --> 00:34:06,960
+قسطرة بولية اللي بيدخل أنبوب إلى داخل المثانة عبر
+
+461
+00:34:06,960 --> 00:34:12,220
+اليوريثرا سواء male أو female هيلاقي أنه الشخص هذا
+
+462
+00:34:12,220 --> 00:34:16,480
+اللي دخله قسطرة صار عنده urinary tract
+
+463
+00:34:16,480 --> 00:34:21,950
+infection باستمرار ليش؟ لأنه القسطرة .. الجزء اللي 
+
+464
+00:34:21,950 --> 00:34:27,650
+دخل جوا من القسطرة أصبح matrix، يعني مكان للـ
+
+465
+00:34:27,650 --> 00:34:31,270
+biofilm للبكتيريا، بنعالجه، بنقتل البكتيريا اللي
+
+466
+00:34:31,270 --> 00:34:34,930
+كانت single cells لحالها، اللي عاملة infection،
+
+467
+00:34:34,930 --> 00:34:37,710
+لكن لا أنا أستطيع أقتل مين؟ الـ biofilm اللي موجود
+
+468
+00:34:37,710 --> 00:34:40,770
+طب بنموت البكتيريا، بنطيب المريض، بعد يوم أو
+
+469
+00:34:40,770 --> 00:34:43,370
+يومين، بنتوقع من إيقاف الـ antibiotic، بيرجع مرة
+
+470
+00:34:43,370 --> 00:34:48,550
+أخرى الـ infection خلصت الموضوع، لو جعدنا مئة سنة
+
+471
+00:34:48,550 --> 00:34:52,230
+نتكلم عن الـ attachment، هنلاقي مواضيع نتكلم فيها،
+
+472
+00:34:52,230 --> 00:34:57,350
+من أهمية هذا الموضوع، إذن الـ attachment سواء كان
+
+473
+00:34:57,350 --> 00:35:00,450
+على مستوى الـ pathogenesis أو على مستوى الـ
+
+474
+00:35:00,450 --> 00:35:03,490
+survival، و الـ survival أنا فهمته في المستشفى، أنا
+
+475
+00:35:03,490 --> 00:35:06,070
+الدرس الماضي كان عن الـ disinfection و الـ
+
+476
+00:35:06,070 --> 00:35:08,930
+sterilization، مظبوط ولا لأ؟ إذا ما كانش هندي
+
+477
+00:35:08,930 --> 00:35:12,670
+المقدرة بتعطيكم مثال واقعي من أبحاثنا اللي عملناها
+
+478
+00:35:12,670 --> 00:35:15,450
+كدراسة مجموعة من طالبات قسم العلوم الطبية
+
+479
+00:35:15,450 --> 00:35:19,470
+المخبرية عملوا دراسة على المياه اللي بتشربوها في
+
+480
+00:35:19,470 --> 00:35:22,810
+الجامعة اللي بتلاقوها في الحنفيات أو الـ boxes هذه
+
+481
+00:35:24,380 --> 00:35:28,480
+طبعا مصدرها واحد احنا خدنا من جهنم مصدر بلدية
+
+482
+00:35:28,480 --> 00:35:31,600
+وقعنا مصدر الـ filter وقعنا مصدر بئر .. بئر خاص
+
+483
+00:35:31,600 --> 00:35:34,700
+بالجامعة و أخذوا عينات من كل الأماكن المهم خلينا في
+
+484
+00:35:34,700 --> 00:35:37,780
+موضوعنا المياه اللي أنتم تشربوا منها اللي هي جام
+
+485
+00:35:37,780 --> 00:35:43,000
+الـ فلتر لجينا أن الفلتر بعطي ميه كويسة لكن لجينا
+
+486
+00:35:43,000 --> 00:35:46,740
+بعض الـ .. الوحدات الـ terminals تمام ما بتعطيش ميه
+
+487
+00:35:46,740 --> 00:35:50,940
+كويسة فيها micro organisms فأطول بقتصرنا في شغل
+
+488
+00:35:50,940 --> 00:35:53,940
+إدارية وقلنا لهم يا جماعة المياه اللي عندنا في
+
+489
+00:35:53,940 --> 00:35:56,540
+الجامعة في بعض الأماكن واحد اتنين تلاتة أربع عملنا لهم
+
+490
+00:35:56,540 --> 00:36:00,120
+زي خارطة أنه يجب عليكم تتوقفوا الـ system يوم الخميس
+
+491
+00:36:00,120 --> 00:36:04,420
+و الجمعة يصير في الخميس أو الأربعة و الخميس و
+
+492
+00:36:04,420 --> 00:36:08,200
+الجمعة ويصير this disinfection و الجماعة الصحيح
+
+493
+00:36:08,200 --> 00:36:10,720
+كانوا متعاونين جدا الإخوة في شغل إدارية وجاموا
+
+494
+00:36:10,720 --> 00:36:14,560
+بحملة رائعة جدا ونظفوا كل الأماكن مثل البنات يوم
+
+495
+00:36:14,560 --> 00:36:17,120
+السبت وروحوا أخذوا عينات هتلاقوا البكتيريا أكثر من
+
+496
+00:36:17,120 --> 00:36:17,320
+دقيقة
+
+497
+00:36:20,370 --> 00:36:23,250
+جمعوا عينات بالفعل لو البكتيريا عددها أكبر من ذي
+
+498
+00:36:23,250 --> 00:36:29,010
+قبل، إيش اللي صار؟ أنه الأماكن هذه اللي عملوا فيها
+
+499
+00:36:29,010 --> 00:36:33,370
+عملية تنظيف هم أثاروا الـ biofilms، يعني عملوا تنظيف
+
+500
+00:36:33,370 --> 00:36:36,630
+فالـ biofilm اللي كانت راكدة و تعطي عدد معين من 
+
+501
+00:36:36,630 --> 00:36:41,410
+البكتيريا باستمرار، مش استفزوها، أثروها بمعنى أن
+
+502
+00:36:41,410 --> 00:36:45,210
+هم بنفسهم أزقوها في مضيقها الآن صار مورد الآن
+
+503
+00:36:45,210 --> 00:36:48,190
+بالسائق، فقالوا مضطرب في ال system كمان مرة وضخوا
+
+504
+00:36:48,190 --> 00:36:51,230
+مياه سخنة في ال system وضخوا مياه .. أو chlorine 
+
+505
+00:36:51,230 --> 00:36:53,590
+dosage  جرعة عالية وبعدين يغسلوا ال system و
+
+506
+00:36:53,590 --> 00:36:57,410
+بالفعل صارت عملية التنظيف، القصد من الكلام أنه
+
+507
+00:36:57,410 --> 00:37:01,070
+طريقة التنظيف اختلفت لأن البكتيريا
+
+508
+00:37:01,070 --> 00:37:05,610
+كانت عاملة إيه؟ بيوم، آه دي نقطة كثيرة، يبقى ال
+
+509
+00:37:05,610 --> 00:37:06,550
+attachment  صفات
+
+510
+00:37:09,760 --> 00:37:14,240
+ميزة للبكتيريا إذا انتلقتها .. انتلقت يعني صفات
+
+511
+00:37:14,240 --> 00:37:21,420
+إيجابية لها تؤهلها لل survival بشكل جيد طيب
+
+512
+00:37:21,420 --> 00:37:24,980
+النقطة الثانية اللي بعد ال attachment اللي هي ال
+
+513
+00:37:24,980 --> 00:37:29,360
+invasion of host cell antigen اتفقنا إنه في
+
+514
+00:37:29,360 --> 00:37:33,840
+إمكانية لل invasion أو localized infection خلينا
+
+515
+00:37:33,840 --> 00:37:36,660
+نجسم ال infection نسميها localized و
+
+516
+00:37:36,660 --> 00:37:40,460
+systemic إيش يعني localized؟ يعني محددة في local
+
+517
+00:37:40,460 --> 00:37:44,300
+area معينة زي التيتانوس ما كنا نقول أصعب منطقة من
+
+518
+00:37:44,300 --> 00:37:47,900
+الجرح أو من العضل وبقية هناك بينما السايمونيلا
+
+519
+00:37:47,900 --> 00:37:52,000
+تايفية انتقلت من ال intestine إلى ال blood إلى ال
+
+520
+00:37:52,000 --> 00:37:55,760
+gallbladder إلى ال central nervous system وهذا
+
+521
+00:37:55,760 --> 00:38:00,420
+يسميها systemic ما الذي يؤهل البكتيريا إنها تعمل
+
+522
+00:38:00,420 --> 00:38:01,440
+invasion؟
+
+523
+00:38:06,440 --> 00:38:13,660
+لكن لا بأس إننا نتطلع على المسميات وبعدين نناقش ال
+
+524
+00:38:13,660 --> 00:38:19,120
+function تبع كل مسمى منهم الاسم الأول
+
+525
+00:38:19,120 --> 00:38:24,420
+Hyaluronidase، الاسم الثاني collagenase، الاسم
+
+526
+00:38:24,420 --> 00:38:29,180
+الثالث neuroaminidase، الرابع coagulase، الخامس
+
+527
+00:38:29,180 --> 00:38:34,720
+kinase، لاحظوا الشغل؟ كلهم ases يعني enzymes وكل 
+
+528
+00:38:34,720 --> 00:38:39,000
+واحد اسمه معاه اسمه معاه coagulation بيعمل
+
+529
+00:38:39,000 --> 00:38:44,700
+coagulation ال kinases ما بتعرفوهاش بتحلل نعم بتحلل
+
+530
+00:38:44,700 --> 00:38:50,460
+ال clots عكس ال coagulase بره لابن الجرح كيف الرسم
+
+531
+00:38:50,460 --> 00:38:53,700
+بيعمل healing؟ أحد بيعمل ال clotting بيعمل ال
+
+532
+00:38:53,700 --> 00:38:57,620
+clotting مظبوط ولا لأ؟ تخيلوا لو بكتيريا من النوع
+
+533
+00:38:57,620 --> 00:39:02,500
+اللي بنتج kinase قلت واستقرت في الجرح كل ما يتكون
+
+534
+00:39:02,500 --> 00:39:07,460
+ال clot هتعملها .. هتعمل عملية تحليل للجلطة على
+
+535
+00:39:07,460 --> 00:39:11,000
+فكرة هذه استخدامات ظاهرة في علاج أمراض الجلطة طبعا
+
+536
+00:39:11,000 --> 00:39:14,060
+هيروح على المستشفى على مرض القلب يلاقي عندهم علاج
+
+537
+00:39:14,060 --> 00:39:17,860
+غير ثمين جدا غير اسمه streptokinase streptokinase
+
+538
+00:39:17,860 --> 00:39:21,720
+مأخوذ من streptococcus من streptococcus حينك سموه
+
+539
+00:39:21,720 --> 00:39:25,480
+streptokinase في استفيدوا كينيز لكن لا يستخدم لا
+
+540
+00:39:25,480 --> 00:39:31,120
+يستخدم ال coagulase .. ال coagulase بيحول ال
+
+541
+00:39:31,120 --> 00:39:38,100
+fibrinogen إلى fibrin عكس اللي هو ال kinase تخيلوا
+
+542
+00:39:38,100 --> 00:39:41,860
+ال Staphylococcus aureus تنتج coagulase والموضوع
+
+543
+00:39:41,860 --> 00:39:45,440
+ده في دمنا وكل ما جاب ال fibrinogen بتحوله ل
+
+544
+00:39:45,440 --> 00:39:49,400
+fibrin يعني هتعمل clots في الجسم، هتعمل جلطات في
+
+545
+00:39:49,400 --> 00:39:53,180
+الجسم طبعا هي .. هي مش قصدها إنها تقتل ال host في
+
+546
+00:39:53,180 --> 00:39:55,920
+الإطار وإنما قصدها تحمي نفسها .. احنا بدنا ندافع
+
+547
+00:39:55,920 --> 00:39:59,320
+عن البكتيريا .. احنا الكل بي .. الكل ضدها .. احنا
+
+548
+00:39:59,320 --> 00:40:02,060
+على الأقل نكون يعني من الناس اللي بيدافع عن البكتيريا
+
+549
+00:40:02,060 --> 00:40:06,000
+.. هي تقصد إنها تحمي نفسها من اللي هو ال immune
+
+550
+00:40:06,000 --> 00:40:09,940
+system تبع الإنسان إنها تكون داخل شيء لزج .. داخل
+
+551
+00:40:09,940 --> 00:40:13,680
+شيء لزج اللي هو ال blood فهو بيحميها من ال immune
+
+552
+00:40:13,680 --> 00:40:17,520
+system .. لكن دون أن تقصد قد تقتلنا .. مافي مشكلة
+
+553
+00:40:17,520 --> 00:40:20,280
+.. يعني مبدأ مش دون قصد .. يعني ال D تبعت غير ..
+
+554
+00:40:20,550 --> 00:40:26,190
+الضرر تبعتها رهيب طيب .. طب مين .. مين .. مين
+
+555
+00:40:26,190 --> 00:40:29,590
+بالكه أنجح؟ ال host .. آه عفوا .. ال .. ال .. ال
+
+556
+00:40:29,590 --> 00:40:34,910
+.. ال pathogen القاتل ولا ال pathogen اللي ممكن
+
+557
+00:40:34,910 --> 00:40:40,330
+يعيش معانا بدون ما يقتلنا؟ إنه أنجح؟ من وجه نظر ..
+
+558
+00:40:40,330 --> 00:40:43,390
+من وجه نظر ال pathogen نفسه؟ إنه أحسن له؟
+
+559
+00:40:45,860 --> 00:40:51,000
+يعني بما إنه بيسكت وبيبقيينا حيّان لإنه هيطول من
+
+560
+00:40:51,000 --> 00:40:56,400
+فترة حياته هو، بيطول فترة حياتنا احنا، أما لو قتلنا
+
+561
+00:40:56,400 --> 00:41:00,240
+تمام زي ال anthrax فهو .. احنا هنموت لأن هو مش
+
+562
+00:41:00,240 --> 00:41:03,960
+هلاقي غذاء وكده ليه هيموت، إذًا الأنجح له إنه ..
+
+563
+00:41:04,250 --> 00:41:08,970
+يبقى زي .. زي ال .. البكتيروفاج لما دخل الخلية وجاءهم
+
+564
+00:41:08,970 --> 00:41:13,310
+أنا بس ما عليش يعني .. مؤقتًا يعني .. آه وإن
+
+565
+00:41:13,310 --> 00:41:16,950
+انقسم من انقسام الخلية، هذا كان أنجح من مين؟ من أخوه
+
+566
+00:41:16,950 --> 00:41:23,470
+اللي دخل وحلل الخلية بشكل عنيف فين حالة اسمها
+
+567
+00:41:23,470 --> 00:41:27,210
+streptomycin؟ عفوا، leukocidin، leukocidin
+
+568
+00:41:27,210 --> 00:41:31,910
+leukocidin كلمة .. cidin معناها .. جاية من
+
+569
+00:41:31,910 --> 00:41:37,020
+كلمة cidal، cidal القتل leukو أبيض القاتل الأبيض
+
+570
+00:41:37,020 --> 00:41:40,940
+يعني مسبب قتل كرات الدم البيضاء قاتل لكرات
+
+571
+00:41:40,940 --> 00:41:45,160
+الدم البيضاء هذا متخصص البكتيريا تنتجه عشان يقتل
+
+572
+00:41:45,160 --> 00:41:48,020
+كرات الدم البيضاء عشان نعرف إن كرات الدم البيضاء
+
+573
+00:41:48,020 --> 00:41:54,280
+المقصودة ال macrophage وال phagocytes كلها من
+
+574
+00:41:54,280 --> 00:41:58,140
+اللي تنتمي إلى ال white blood cell فلما نلاقي خلية
+
+575
+00:41:58,140 --> 00:42:02,320
+قادرة على قتل ال white blood cell هذا مؤشر يعني
+
+576
+00:42:02,320 --> 00:42:06,360
+أنه البكتيريا عندها virulence factor نيجي لل
+
+577
+00:42:06,360 --> 00:42:09,200
+streptolysin وال hemolysin الصحيح إنه .. يعني
+
+578
+00:42:09,200 --> 00:42:13,220
+ال hemolysin أشمل لأن ال streptolysin هو hemolysin
+
+579
+00:42:13,220 --> 00:42:17,480
+بس تنتجه اللي هو ال streptococcus يعني متخصص بال
+
+580
+00:42:17,480 --> 00:42:22,560
+streptococcus hemolysin بيعمل hemolysis لمين؟ لل ..
+
+581
+00:42:22,560 --> 00:42:26,080
+هو لل Red Blood Cell أكثر شيء عشان يسموه hemo ..
+
+582
+00:42:26,080 --> 00:42:30,870
+hemo وال hem في ال Red Blood Cell طبعا
+
+583
+00:42:30,870 --> 00:42:33,670
+ال streptolysin هو hemolysin بإنضمام مرة بس بتنتج
+
+584
+00:42:33,670 --> 00:42:38,390
+ل streptococcus لسه تنايز و phospholipases برضه نفس
+
+585
+00:42:38,390 --> 00:42:43,530
+الفكرة ال .. ال .. ال .. ال phospholipases اسم عام
+
+586
+00:42:43,530 --> 00:42:46,910
+لمجموعة من الإنزيمات قادرة على تحليل ال
+
+587
+00:42:46,910 --> 00:42:50,330
+phospholipids أحد ال phospholipids المشهورة اللي هو
+
+588
+00:42:50,330 --> 00:42:54,990
+ال lecithin بتنتجه بكتيريا زي كلوسترديوم بنسميه
+
+589
+00:42:54,990 --> 00:43:00,150
+lecithinase واسم ثاني حتى phospholipase C كده ال
+
+590
+00:43:00,150 --> 00:43:04,330
+lecithin أو عفوا lecithinases هي تبع لمين؟ لل
+
+591
+00:43:04,330 --> 00:43:10,460
+phospholipase في عندنا anthrax edema factor و
+
+592
+00:43:10,460 --> 00:43:15,900
+pertussis adenylate cyclase enzyme AC اختصار ال
+
+593
+00:43:15,900 --> 00:43:20,020
+adenylate cyclase enzyme ناخد الأخير adenylate
+
+594
+00:43:20,020 --> 00:43:23,660
+cyclase enzyme بتعرفوا حاجة اسمها Cyclic AMP و GMP؟
+
+595
+00:43:23,660 --> 00:43:29,500
+Cyclic AMP و GMP طيب بتعرفوا حاجة اسمها ATP؟ ال
+
+596
+00:43:29,500 --> 00:43:34,900
+Cyclic AMP و GMP أحد منتجات اللي هو ال ATP خذ بالك ال
+
+597
+00:43:34,900 --> 00:43:39,700
+Cyclic AMP الوظائف رائعة جدا في الجسم ومحددة
+
+598
+00:43:39,700 --> 00:43:47,640
+وكميته على قدر وظيفته إذا زادت كميته زادت وظيفته
+
+599
+00:43:47,640 --> 00:43:52,620
+ومشكلة إنه تزيد الوظيفة أنا بدي أنتج عشر مركبات في
+
+600
+00:43:52,620 --> 00:43:56,920
+اليوم هاي طاقة الإنتاجية وبيجيبوا بالمهمة تماما
+
+601
+00:43:56,920 --> 00:43:58,300
+عشرة لو أنتجت مية
+
+602
+00:44:01,530 --> 00:44:04,590
+يبقى مانع أكثر في خلل في ال system اللي أنا مخططه
+
+603
+00:44:04,590 --> 00:44:09,490
+عشان أنت فقط عشان ال adenylate cyclase enzyme
+
+604
+00:44:09,490 --> 00:44:15,850
+بيعمل نشاط معين يؤدي هذا النشاط إلى إنتاج كمية
+
+605
+00:44:15,850 --> 00:44:20,290
+كبيرة من من؟ من ال cyclic AMP فال cyclic AMP بدل
+
+606
+00:44:20,290 --> 00:44:22,950
+ما يجم بالوظيفة المحدودة للجدي صارت تجري بالوظيفة
+
+607
+00:44:22,950 --> 00:44:28,000
+الكبيرة فتؤدي إلى خلل كلمة خلل ما أنتم عارفين؟ أعطيكم
+
+608
+00:44:28,000 --> 00:44:30,620
+مثال على adenylate cyclase enzyme ال Vibrio cholera
+
+609
+00:44:30,620 --> 00:44:35,820
+بتنتج cholera toxin لو وصفناه احنا بنوصفه على أنه
+
+610
+00:44:35,820 --> 00:44:41,640
+adenylate cyclase enzyme بيرتبط التكسين بالأديناليت
+
+611
+00:44:41,640 --> 00:44:46,420
+سايكليز بيعمل له activation لما يصير activation
+
+612
+00:44:46,420 --> 00:44:51,240
+لل adenylate cyclase enzyme بيتحول ال ATP ل cyclic
+
+613
+00:44:51,240 --> 00:44:54,620
+AMP يبقى صرفين ينتج كمية كبيرة من مين؟ من ال
+
+614
+00:44:54,620 --> 00:44:59,260
+cyclic AMP الآن ال enterotoxin بيمنع ال absorption،
+
+615
+00:44:59,260 --> 00:45:02,340
+مهمته يمنع ال absorption تبع ال chloride ويزيد
+
+616
+00:45:02,340 --> 00:45:05,800
+من ال secretion تبع إيه؟ الصوديوم، لما أصير في
+
+617
+00:45:05,800 --> 00:45:10,020
+عندي صوديوم في هذه الأمعاء، تخيلوا الأمعاء مصورة،
+
+618
+00:45:10,020 --> 00:45:13,640
+صار عندي عدم امتصاص لل chloride وإنتاج لمين؟ لل
+
+619
+00:45:13,640 --> 00:45:17,820
+sodium، فصار عندي كمية كبيرة من ال chloride جوا وال
+
+620
+00:45:17,820 --> 00:45:22,400
+sodium، ال chloride وال sodium وال calcium،
+
+621
+00:45:22,400 --> 00:45:25,910
+هدول عبارة عن electrolytes إذا زادت كمية الـ
+
+622
+00:45:25,910 --> 00:45:29,070
+electrolytes في ال human of the intestine الجسم
+
+623
+00:45:29,070 --> 00:45:34,030
+بيشعر إن هذا خطر يهدد حياة الإنسان فبيروح بينتج
+
+624
+00:45:34,030 --> 00:45:37,630
+كمية كبيرة من إيه؟ من السائل اللي هو الميه to
+
+625
+00:45:37,630 --> 00:45:42,890
+dilute لإخفف دياريا is produced وصلت الصورة 
+
+626
+00:45:42,890 --> 00:45:48,630
+فتخيلوا إن هذا الانزيم اللي هو غليز انزايم مش موجود له
+
+627
+00:45:48,630 --> 00:45:52,370
+تجويف في المكان اللي هو فيه زي الـ pertosis مثلا،
+
+628
+00:45:52,370 --> 00:45:57,130
+تمام؟ الحقيقة ما تقلش الآن ما يسمى بالـ edema اللي هي
+
+629
+00:45:57,130 --> 00:46:02,390
+الانتفاخ في الاستسقاء اللي هو إنه مية تنزل في
+
+630
+00:46:02,390 --> 00:46:05,330
+الأنسجة extra cellular water لأنه برضه عملية
+
+631
+00:46:05,330 --> 00:46:09,130
+dilution لمين؟ ده الـ Electrolyte المرة القادمة إن
+
+632
+00:46:09,130 --> 00:46:14,210
+شاء الله بنأكد على بعض الزيادة تبع الـ virus factor
+
+633
+00:46:14,210 --> 00:46:17,790
+هذا اللي هنتكلم فيه وبعض الناس هياخد معانا فترة
+
+634
+00:46:17,790 --> 00:46:22,640
+طويلة اللي بناخده في أول المحاضرة هيزمها في
+
+635
+00:46:22,640 --> 00:46:24,440
+المحاضرة التانية أو بالتالتة أو بالرابعة أو
+
+636
+00:46:24,440 --> 00:46:27,340
+بالخامسة فأنا أرجو من حضراتكم إن أنتم تقرأوا معانا 
+
+637
+00:46:27,340 --> 00:46:27,740
+باسم الله
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/ipIvFNbRCx0_postprocess.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/ipIvFNbRCx0_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..5b502e69fc29405e9c254e6cde0e6ece830c26ca
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/ipIvFNbRCx0_postprocess.srt
@@ -0,0 +1,2620 @@
+1
+00:00:21,720 --> 00:00:24,480
+بسم الله الرحمن الرحيم بالبدأ بقولكوا كل عامة
+
+2
+00:00:24,480 --> 00:00:28,660
+وبخير عودة من الإجازة يعني بيكون الإنسان شوية كسول
+
+3
+00:00:28,660 --> 00:00:31,020
+فاعطونا يوم الأربع عشان نتنشط ليوم السبت إن شاء
+
+4
+00:00:31,020 --> 00:00:36,900
+كنا بدأنا الحديث المرة الماضية على جزئية متعلقة
+
+5
+00:00:36,900 --> 00:00:39,960
+بال artificial cultivation of microorganisms وكنا
+
+6
+00:00:39,960 --> 00:00:43,260
+إن كل اللي درسنا من هذا ال chapter أساسا موظف
+
+7
+00:00:43,260 --> 00:00:47,680
+لخدمة اللي هو هذا الجزء من المحاضرة إنه كيف نمي ال
+
+8
+00:00:47,680 --> 00:00:53,560
+microorganismوتحدثنا عن أنواع و تصنيفات لـ Media
+
+9
+00:00:53,560 --> 00:00:56,480
+زي الـ Minimal Essential Medium زي الـ Enrichment
+
+10
+00:00:56,480 --> 00:01:00,640
+Media زي الـ Selective و الـ Differential Media
+
+11
+00:01:01,460 --> 00:01:05,200
+واللوحة أمامكم بتقول أنه فيه تلت أصناف من ال media
+
+12
+00:01:05,200 --> 00:01:08,200
+based on physical condition، يا إما solid، يا إما
+
+13
+00:01:08,200 --> 00:01:13,280
+liquid، يا إما semi-solid، والتلت أشكال أكيد مدام
+
+14
+00:01:13,280 --> 00:01:17,120
+موجودين يبجي إليهم استخداماتهم، أحد الأشياء اللي
+
+15
+00:01:17,120 --> 00:01:22,160
+لازم نأكد عليها اللي هو ال solid mediaبتلاحظوا
+
+16
+00:01:22,160 --> 00:01:25,720
+الآن اللي هو اللي قدامكوا فيه طبقين الطبق الأول كل
+
+17
+00:01:25,720 --> 00:01:29,280
+المستعمرات فيها لون أبيض و أصل الوسط لونه شفاف
+
+18
+00:01:29,280 --> 00:01:33,780
+الوسط والتانية فيه أسفر و فيه أحمر فيه أكتر من لون
+
+19
+00:01:33,780 --> 00:01:37,920
+فيه ال .. ال .. الوسط اللي بيحتوي على أكتر من لون
+
+20
+00:01:37,920 --> 00:01:42,360
+ماذا يعني ذلك؟ أنه فيه أكتر من نوع بكتيريا يبقى
+
+21
+00:01:42,360 --> 00:01:46,180
+البكتيريا اللي زرعت على هذا الطبق لم تكن pure ماذا
+
+22
+00:01:46,180 --> 00:01:50,890
+نقصد ب pure؟معناه نقية، نقية يعني مستعمرة لا تحتوي
+
+23
+00:01:50,890 --> 00:01:55,950
+إلا على صنف واحد من البكتيريا، غالبا احنا كيف في
+
+24
+00:01:55,950 --> 00:01:58,630
+المعمل بنحصل على صنف واحد من البكتيريا أو pure
+
+25
+00:01:58,630 --> 00:02:06,260
+culture؟ بنفترض جدا ان كل مستعمرة نشأت منخلية
+
+26
+00:02:06,260 --> 00:02:10,240
+واحدة يبقى كل مستعمرة أمامك هنا على الصبورة أو على
+
+27
+00:02:10,240 --> 00:02:13,380
+اللوحة تمام؟ زي مثلا اللي .. اللي .. اللي .. اللي
+
+28
+00:02:13,380 --> 00:02:17,840
+بنشعلها بالسهم هذه نشعت من إيه؟ هذا افتراض جدلي
+
+29
+00:02:17,840 --> 00:02:22,660
+صحيح بنسبة كبيرة جدا لكن مش absolute لكن احنا
+
+30
+00:02:22,660 --> 00:02:26,080
+ماشينا عليه بمعنى انه لأن ممكن اتنين بكتيريا
+
+31
+00:02:26,080 --> 00:02:32,130
+يكونوا مستعمرة واحدة وبتالي هو افتراض جدليلكن هذه
+
+32
+00:02:32,130 --> 00:02:35,430
+احد ميزات ال solid media ايش ميزات ال solid media
+
+33
+00:02:35,430 --> 00:02:39,810
+انه ال growth تبع البكتيريا بيكون بشكل ال colonies
+
+34
+00:02:39,810 --> 00:02:43,530
+طب ما فاكرت ال colonies؟ واحد متأكد من ال purity
+
+35
+00:02:43,530 --> 00:02:49,230
+of the culture لقم اتنين ال identity of the
+
+36
+00:02:49,230 --> 00:02:53,070
+microorganism could be identified using cultural
+
+37
+00:02:53,070 --> 00:02:56,860
+characteristics ليش يعني؟يعني الصفات المزرعية اللي
+
+38
+00:02:56,860 --> 00:03:01,200
+هي شكل المستعمرة، ارتفاعها، لونها، حجمها، ال
+
+39
+00:03:01,200 --> 00:03:05,600
+texture تبعتها، القوام تبعتها، هل هو لزج، هل هو
+
+40
+00:03:05,600 --> 00:03:11,020
+watery، هل هو dry، هل هو wet، هل هي حمراء، خضراء،
+
+41
+00:03:11,020 --> 00:03:15,440
+بيضاء، مرتفعة، إلها قبة، هذي يسميها أشكال
+
+42
+00:03:15,440 --> 00:03:20,830
+المستعمرةكل نوع معين من البكتيريا إيه له شكل محدد
+
+43
+00:03:20,830 --> 00:03:24,650
+من إيه؟ من المستعمرات فإذا رأيت الشكل المحدد
+
+44
+00:03:24,650 --> 00:03:29,230
+تعرفتي على مين؟ على النوع وبتلاقي ميزة لل solid
+
+45
+00:03:29,230 --> 00:03:32,810
+media إن النمو بيكون فيها بشكل مستعمرات بينما ال
+
+46
+00:03:32,810 --> 00:03:36,570
+liquid media النمو فيها بيكون بشكل turbidity إيش
+
+47
+00:03:36,570 --> 00:03:40,050
+يعني turbidity؟ يعني أكورة بمعنى إنه من طالع
+
+48
+00:03:40,050 --> 00:03:44,670
+الأنبوبة بنشوف إنه إذا فيش growth، no turbidity،
+
+49
+00:03:44,670 --> 00:03:45,930
+في growth، في turbidity
+
+50
+00:03:48,650 --> 00:03:52,250
+هي أكورة بتختلف عن أكورة أخرى، التربيت كلها
+
+51
+00:03:52,250 --> 00:03:57,270
+تتشابه، إذن هي أول advantage لل solid media عالمين
+
+52
+00:03:57,270 --> 00:04:01,130
+على ال liquid media إنه النمو بيكون شكل مستعمرات
+
+53
+00:04:01,130 --> 00:04:04,690
+طب ما هي ال advantage تبع ال liquid media على ال
+
+54
+00:04:04,690 --> 00:04:08,850
+solid media؟ أحد أهم ال advantages اللي هو سرقة
+
+55
+00:04:08,850 --> 00:04:13,680
+النموليش سرقت النمو؟ البكتيريا في ال liquid media
+
+56
+00:04:13,680 --> 00:04:16,780
+is in direct contact .. in direct contact يعني على
+
+57
+00:04:16,780 --> 00:04:20,080
+اتصال مباشر مع مين؟ مع ال nutrient .. بينما في ال
+
+58
+00:04:20,080 --> 00:04:23,400
+solid media .. طلع ال solid media .. هل ال
+
+59
+00:04:23,400 --> 00:04:26,640
+nutrient اللي في المنطقة هذه available للمستعمر
+
+60
+00:04:26,640 --> 00:04:30,760
+هذه؟ مش available .. لكن لو كانت في liquid .. إيش
+
+61
+00:04:30,760 --> 00:04:35,180
+بيعمل ال liquid؟لو البكتيريا .. لو البكتيريا
+
+62
+00:04:35,180 --> 00:04:40,300
+متحركة، هتنتقل من مكان إلى آخر، طب لو غير متحركة،
+
+63
+00:04:40,300 --> 00:04:43,660
+جزيئات السائل لها حركة، بيسموها .. بيسموها
+
+64
+00:04:43,660 --> 00:04:47,560
+Brownian Movement of Water Molecules، برضه هتنقل
+
+65
+00:04:47,560 --> 00:04:52,620
+البكتيريا من مكان إلى مكان آخر، إذا في ال .. في ال
+
+66
+00:04:52,620 --> 00:04:55,520
+liquid media، إذا أنا بدي أنمي البكتيريا بشكل
+
+67
+00:04:55,520 --> 00:05:00,080
+سريع، بنميها في liquid mediaإذا بدى أحصل على
+
+68
+00:05:00,080 --> 00:05:04,200
+مصطعمرات عشان أحصل على pure culture أو أحصل على
+
+69
+00:05:04,200 --> 00:05:08,280
+identification للبكتيريا بنميها على solid medium
+
+70
+00:05:08,280 --> 00:05:14,720
+قدامكوا لوحة بتتكلم عن ال semi-solid medium ال
+
+71
+00:05:14,720 --> 00:05:19,920
+semi-solid غرض أساسى إلها to detect the motility
+
+72
+00:05:19,920 --> 00:05:23,920
+of bacteriaفاكريني لما اتكلمنا عن ال flagella و أن
+
+73
+00:05:23,920 --> 00:05:28,840
+البكتيريا بتتحرك بال flagella سباحة ولا طيران ولا
+
+74
+00:05:28,840 --> 00:05:34,640
+ماشي تسبح و السباحة بتكون في إيه؟ في سائل أنا لسه
+
+75
+00:05:34,640 --> 00:05:39,520
+قبل دقيقة بقول إنه البكتيريا الغير متحركة قادرة
+
+76
+00:05:39,520 --> 00:05:43,120
+على الانتقال في السائل من مكان إلى مكان بذال حركة
+
+77
+00:05:43,120 --> 00:05:46,340
+دذائقات في السائل إذا كيف بتقعد من البكتيريا
+
+78
+00:05:46,340 --> 00:05:50,840
+متحركة أو غير متحركة؟ ع الصلب بتمشيشو في ال liquid
+
+79
+00:05:50,840 --> 00:05:55,160
+هتختلط الحابل منها بنظرا ليه حركة وجهة السائل
+
+80
+00:05:55,160 --> 00:05:58,620
+فصممنا وسط ما بينهما اللي هو ال semi-solid media
+
+81
+00:05:58,620 --> 00:06:01,740
+طلعوا على الأنبوبة ها دي اللي هي أمامنا الآن اللي
+
+82
+00:06:01,740 --> 00:06:06,300
+باللون الأحمرها زرعنا البكتيريا بشكل طعنة step بعد
+
+83
+00:06:06,300 --> 00:06:09,160
+ما نمت البكتيريا نمت في نفس مكان الطعنة دلوقت على
+
+84
+00:06:09,160 --> 00:06:13,340
+أنه لم تتحرك عن نطاق خط الطعنة بينما في الأنبوبة
+
+85
+00:06:13,340 --> 00:06:18,590
+الثانيةطب عاملناها بنفس الكيفية لكن لاحظنا الأن
+
+86
+00:06:18,590 --> 00:06:23,950
+انتشار للبكتيريا دلوقتي البكتيريا متحرك وهذا ما
+
+87
+00:06:23,950 --> 00:06:27,130
+نقوم به في فحص ال motility في المعمل في ال semi
+
+88
+00:06:27,130 --> 00:06:32,530
+-solid إذا ال liquid و ال solid مهمين و كمان ال
+
+89
+00:06:32,530 --> 00:06:38,190
+semi-solid أيضا له أهمية خاصةلماذا نقوم بزراعة
+
+90
+00:06:38,190 --> 00:06:42,090
+البكتيرا في المعمل؟ كمان مرة سؤال لازم نجاوب عليه
+
+91
+00:06:42,090 --> 00:06:45,950
+و نكون فاهمين إن أحنا مازرعهاش عشان نتسلّى و نشوف
+
+92
+00:06:45,950 --> 00:06:50,550
+ألوانها الجذابة، لأ وإنما لأغراض محددة واحد
+
+93
+00:06:50,550 --> 00:06:57,910
+isolation and identification لازم نعرف البكتيرا
+
+94
+00:06:57,910 --> 00:07:03,810
+تخيلوا معاه الوضع التالي تسمعوا بمرض اتهاب الصحية
+
+95
+00:07:03,810 --> 00:07:09,270
+اللي هو ال meningitesمن الذي يسبب ال meningitis؟
+
+96
+00:07:09,270 --> 00:07:15,110
+طلعوا الأصناع في الأربع، بكتيريا، بكتيريا،
+
+97
+00:07:15,110 --> 00:07:19,990
+viruses، parasites و fungi طالما بنقول نسينا من
+
+98
+00:07:19,990 --> 00:07:23,490
+التلات و نخدنا في واحد، بكتيريا، هل البكتيريا نوع
+
+99
+00:07:23,490 --> 00:07:26,770
+واحد؟ Staphylococcus aurea, pseudomonas
+
+100
+00:07:26,770 --> 00:07:29,230
+aeruginosa, Escherichia coli, Hemophilus
+
+101
+00:07:29,230 --> 00:07:32,050
+influenza, Neisseria meningitis, Certococcus
+
+102
+00:07:32,050 --> 00:07:34,770
+pneumonia، مجموعة ضخمة من البكتيريا كلها بتسبب مرض
+
+103
+00:07:34,770 --> 00:07:40,360
+واحد اسمه إيه؟ meningitisوالطبيب بيشخص مرض اسمه
+
+104
+00:07:40,360 --> 00:07:45,520
+meningitis بس مابشخصش المسبب تبع ال meningitis انا
+
+105
+00:07:45,520 --> 00:07:47,880
+اتكلمت من الأسماء اللي ذكرتها في منها gram
+
+106
+00:07:47,880 --> 00:07:51,340
+negative ومنها gram positive حتى العلاج تبعها هبقى
+
+107
+00:07:51,340 --> 00:07:54,080
+اختلف ال antibiotic تبعها هبقى اختلف وبالتالي
+
+108
+00:07:54,080 --> 00:07:58,560
+عملية عزل ال micro و التأكد من هويته مهم جدا في
+
+109
+00:07:58,560 --> 00:08:03,370
+اختيارالمروض الحياة، بالمناسبة ده واحد، رقم اتنين
+
+110
+00:08:03,370 --> 00:08:07,210
+for epidemiological وهدي نقطة سأكررها مرارا او
+
+111
+00:08:07,210 --> 00:08:12,150
+تكرارا في الفصل هذا و الفصل القادر For
+
+112
+00:08:12,150 --> 00:08:17,570
+epidemiological reason، الان لو سألنا سؤال عام، ما
+
+113
+00:08:17,570 --> 00:08:25,410
+هو اكثر مسبب لاتسمم الغدائد في فلسطين؟ليه أحد
+
+114
+00:08:25,410 --> 00:08:30,490
+يعلم؟ عارفين ليش؟ لأنه لا يتم التحقق ولا يتم عزل
+
+115
+00:08:30,490 --> 00:08:33,870
+وتعريف الميكروبات المسببة لمن؟ لتسلمونيه طبتاني مش
+
+116
+00:08:33,870 --> 00:08:36,870
+هاعرف الإجابة على كثير من الأسئلة المتعلقة
+
+117
+00:08:36,870 --> 00:08:40,970
+بالوبائيات طالما ما أنا ماعنديش معرفة بالتعرف على
+
+118
+00:08:40,970 --> 00:08:45,690
+الميكروبات المسببة لها الأكبر وانتشارها مين أكتر
+
+119
+00:08:45,690 --> 00:08:51,700
+انتشارا في السلمونيلا؟ خانيونس ولا غزين؟عندي معرفة
+
+120
+00:08:51,700 --> 00:08:56,700
+خانيونس تمام؟ أكتر من غزة، ليش؟ لإنه بيتم عزل
+
+121
+00:08:56,700 --> 00:08:59,220
+الميكروب من الستول تبع الأطفال اللي عندهم ديارية
+
+122
+00:08:59,220 --> 00:09:01,580
+أو الأشخاص اللي عندهم ديارية وبالتالي في عندي
+
+123
+00:09:01,580 --> 00:09:05,320
+إحصائية بتقول إن نسبة العزل للسلمونيلا في خانيونس
+
+124
+00:09:05,320 --> 00:09:09,300
+أعلى منها في مدينة غزة، يبقى لما يكون عندي معلومات
+
+125
+00:09:09,300 --> 00:09:15,660
+من هذا النوع أستخدمها في التخطيطوبسأل سؤال لماذا
+
+126
+00:09:15,660 --> 00:09:20,120
+في خانونس مش في غزة؟ لأن جزء كبير من مناطق خانونس
+
+127
+00:09:20,120 --> 00:09:23,160
+غير موصولين بالنظام مجاري مبلغ
+
+128
+00:09:26,450 --> 00:09:30,530
+يصل اكبر عدد ممكن من المنازل اللي فيه خيونس ان انا
+
+129
+00:09:30,530 --> 00:09:34,570
+متأكد ان نسبة الطفليات ونسبة السالمونيلا هتقل فى
+
+130
+00:09:34,570 --> 00:09:39,290
+تلك المنطقة اذا العزل و تعريف الميكروبات مهم جدا
+
+131
+00:09:39,290 --> 00:09:43,090
+لأغراض متنوعة وعديدة منها ال diagnosis منها ال
+
+132
+00:09:43,090 --> 00:09:45,790
+treatment منها ال epidemiological investigation
+
+133
+00:09:45,790 --> 00:09:50,690
+purification انا
+
+134
+00:09:50,690 --> 00:09:55,310
+عندي خلطة من الميكروباتوبدي أعزل واحد منهم، بدي
+
+135
+00:09:55,310 --> 00:09:59,250
+أعزل واحد فقط منهم، يبقى بدي أزرق، عشان أعزله لازم
+
+136
+00:09:59,250 --> 00:10:02,450
+أزرق، لإن في مستعمرة حملة ومستعمرة زرقة ومستعمر
+
+137
+00:10:02,450 --> 00:10:06,650
+مصبوط، بسرهم مع بعض طب ليش بتعمل ال purification؟
+
+138
+00:10:06,650 --> 00:10:10,010
+تخيلوا أنا بدي أنمي بكتيريا عشان أعمل vaccine،
+
+139
+00:10:10,010 --> 00:10:13,050
+vaccine ضد ال Staphylococcus aureus أو ال
+
+140
+00:10:13,050 --> 00:10:16,360
+Staphylococcus pneumoniaبنفع يكون معاه ميكروبات
+
+141
+00:10:16,360 --> 00:10:21,260
+أخرى؟ أنا بنتحقن المريض بهذا أو الشخص بهذا ال ..
+
+142
+00:10:21,260 --> 00:10:22,420
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+143
+00:10:22,420 --> 00:10:22,720
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+144
+00:10:22,720 --> 00:10:23,120
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+145
+00:10:23,120 --> 00:10:23,460
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+146
+00:10:23,460 --> 00:10:24,200
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+147
+00:10:24,200 --> 00:10:24,560
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+148
+00:10:24,560 --> 00:10:24,620
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+149
+00:10:24,620 --> 00:10:27,080
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+150
+00:10:27,080 --> 00:10:35,720
+.. ال .. ال .. ال .. ال .. ال .. ال ..
+
+151
+00:10:35,720 --> 00:10:36,580
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+152
+00:10:36,580 --> 00:10:41,180
+.. ال .. ال
+
+153
+00:10:48,890 --> 00:10:53,370
+تخيل لو انا اعطتك يوم في قنبوبة و قلتلك ايه اعمل
+
+154
+00:10:53,370 --> 00:10:58,490
+الاندول والميتيلريد والروكوسبروسكوار والسيتري ايش
+
+155
+00:10:58,490 --> 00:11:01,910
+النتائج هيطلع؟ positive و positive و positive و
+
+156
+00:11:01,910 --> 00:11:05,890
+positive فهيطلع النتيجة عند اللي قدامي اربعة
+
+157
+00:11:05,890 --> 00:11:08,690
+positive لان انا بتكلم عن مين؟ عن two organism انا
+
+158
+00:11:08,690 --> 00:11:12,910
+افكرهم one organismفانت بديت تطلع على الريداول،
+
+159
+00:11:12,910 --> 00:11:16,290
+اتقارنى مين من الميكروبات اللى بترك هذه الصفات،
+
+160
+00:11:16,290 --> 00:11:19,410
+اربعة بوستين، مش هتلاقي يولا ميكروب، هتقولي هي انا
+
+161
+00:11:19,410 --> 00:11:24,430
+اتشافت ميكروب، ماش دى، اذا احد اهم الأخطاء اللى
+
+162
+00:11:24,430 --> 00:11:28,770
+يمكن نرتكبها ان اتعرف على ميكروبات مش in pure
+
+163
+00:11:28,770 --> 00:11:32,930
+culture، لازم تكون in pure culture، طيب عزلة
+
+164
+00:11:32,930 --> 00:11:36,750
+ميكروب؟بدأ أزرعه عشان بدأ أعمله sensitivity
+
+165
+00:11:36,750 --> 00:11:39,450
+testing، إيش يعني sensitivity testing؟ يعني أشوف
+
+166
+00:11:39,450 --> 00:11:43,450
+مدى حساسيته أو مقاومته لمين، للمضادات، في الحيوان،
+
+167
+00:11:43,450 --> 00:11:48,270
+برضه لازم نزرعه harvesting cells كلمة harvesting
+
+168
+00:11:48,270 --> 00:11:52,820
+وانا حصاد، الحصاد لمين بيكون، للمحصول، صح؟لما انا
+
+169
+00:11:52,820 --> 00:11:56,000
+بدي خلايا انا قلتلك قبل شويه بدي اعمل vaccine صح؟
+
+170
+00:11:56,000 --> 00:12:01,280
+او بدي اعمل reagent باستخدام البكتيريا فبدي خلايا
+
+171
+00:12:01,280 --> 00:12:05,300
+بكتيريا فبزرعها عشان اكلها ثره وانميها و احصد
+
+172
+00:12:05,300 --> 00:12:11,920
+المحصول الwarvesting itself نيجي لللوحة المهمة جدا
+
+173
+00:12:11,920 --> 00:12:17,640
+جدا جدا وهي طريقة تسمياتنا للبكتيريا ايش المحاضرة
+
+174
+00:12:17,640 --> 00:12:22,050
+اللي كنتي؟تسنيم ايش؟ تسنيم الوحيدة ماكان واحدة
+
+175
+00:12:22,050 --> 00:12:24,430
+اسمها في البلد .. في العالم كله تسنيم لوحيدة؟
+
+176
+00:12:24,430 --> 00:12:28,930
+ملايين؟ فيه؟ فيها نفس اسم؟ لأ لوحيدة لأ لوحيدة
+
+177
+00:12:28,930 --> 00:12:34,030
+تسنيم لوحيدة؟ لأ طيب لو كان في عائلتك في كمان عمك
+
+178
+00:12:34,030 --> 00:12:38,330
+مسمى برضه تسنيم تسنيم انت بتختلف عنها صح ولا لأ؟
+
+179
+00:12:38,330 --> 00:12:41,990
+فكيف بنالميزمها من بعض؟ هيك ما هيك هيك خربطونهم في
+
+180
+00:12:41,990 --> 00:12:46,170
+عضو الاسم اسمها واسم أبوها مظبوط التالت والرابع
+
+181
+00:12:46,170 --> 00:12:52,130
+بعض الأحيانففي الميكروبات و لا تشبيه التصنيف بدنا
+
+182
+00:12:52,130 --> 00:12:56,570
+نضمن انه اي فردين بيختلفوا عن بعض يناله اسمين
+
+183
+00:12:56,570 --> 00:12:59,550
+مختلفة تفير ان النظام اسمه binomial system
+
+184
+00:12:59,550 --> 00:13:04,590
+binomial system نظام التسمية الثنائي اللى بيحتوي
+
+185
+00:13:04,590 --> 00:13:09,530
+على اسمين نركز على اللوحة و نشوف فين دي هنا حالة
+
+186
+00:13:09,530 --> 00:13:13,610
+اسمها ال genusو حاجة اسمها Species ال
+
+187
+00:13:13,610 --> 00:13:17,290
+Staphylococcus هو ال genus و ال Aureus هي ال
+
+188
+00:13:17,290 --> 00:13:21,230
+Species اعطيكم مثال يعني في نفس السياق انا بقول
+
+189
+00:13:21,230 --> 00:13:24,070
+Staphylococcus هو ال genus هو اسم العائلة خلاص
+
+190
+00:13:24,070 --> 00:13:29,330
+يعني هو مش عائلة لكن نفترض انه عائلة كتش فيه و ال
+
+191
+00:13:29,330 --> 00:13:33,070
+Aureus هي ال Species هي البنت البنت العائلة في
+
+192
+00:13:33,070 --> 00:13:35,590
+Staphylococcus epidermidis Staphylococcus
+
+193
+00:13:35,590 --> 00:13:38,990
+saprophytic Staphylococcus hemolyticus مين اللي
+
+194
+00:13:38,990 --> 00:13:44,830
+اتغيرالأفراد ال species تمام ال genus هو هو يبقى
+
+195
+00:13:44,830 --> 00:13:49,130
+لو نعرف ال genus هو عبارة عن collection of species
+
+196
+00:13:49,130 --> 00:13:55,450
+مجموعة من ايه؟ من ال species المتشابه إشريشيا
+
+197
+00:13:55,450 --> 00:14:01,110
+كولاي وين ال genus؟ الإشريشيا و الكولاي ال species
+
+198
+00:14:01,110 --> 00:14:07,200
+الآن سلمونيلا طايفيSalmonella Genus والتيفي ال
+
+199
+00:14:07,200 --> 00:14:10,660
+space طبعا ال Salmonella التيفي تيفي موريوم بارا
+
+200
+00:14:10,660 --> 00:14:15,340
+تيفي انترادايدس في مجموعة كبيرة من الأسماء تحت مين
+
+201
+00:14:15,340 --> 00:14:19,760
+تحت اللي هو ال Salmonella اللي بهمني الآن انا
+
+202
+00:14:19,760 --> 00:14:23,280
+انكوا عرفته انه الاسم العلمي للبكتيريا مكون من
+
+203
+00:14:23,280 --> 00:14:28,220
+شقين شق الأول Genus ثاني Space الآن كيف كتبتهم
+
+204
+00:14:28,220 --> 00:14:34,000
+زمان كان في خيارين يما تكتبهم وانا بعمل غلط هنا
+
+205
+00:14:38,240 --> 00:14:50,380
+بدنا نحاول نكتب بطريقة صحيحة شايفين
+
+206
+00:14:50,380 --> 00:14:55,920
+ايش عمل تحتهم انا؟ عمل تحتهم اللي هو كل كلمة منهم
+
+207
+00:14:55,920 --> 00:15:01,640
+خط بسموها underline وكلكم بتعرفوها في ال wordمظبوط
+
+208
+00:15:01,640 --> 00:15:05,420
+او في ال powerpoint بنجيبها من المنطقة هذه و في
+
+209
+00:15:05,420 --> 00:15:09,200
+حاجة جانبها اسمها italic صح اللي هي الخط المائل
+
+210
+00:15:09,200 --> 00:15:13,900
+الاسم العلمي لل bacteria يب يكتب بطريقة اللي
+
+211
+00:15:13,900 --> 00:15:19,320
+مدانكم هذه و هي طريقة جديمة بطل .. بطل حد يستخدمها
+
+212
+00:15:19,320 --> 00:15:22,760
+الان الطريقة المستخدمة هي الطريقة اللي أمامكم هذه
+
+213
+00:15:22,760 --> 00:15:28,120
+حرف الأول capital حرف التانيأو الحرف الأول من
+
+214
+00:15:28,120 --> 00:15:33,540
+الكلمة التانية small والتنتين مكتوبين بخط إيش؟
+
+215
+00:15:33,540 --> 00:15:37,420
+بينهم؟
+
+216
+00:15:37,420 --> 00:15:40,620
+عاملين معالمين احنا اللي انا انا؟ علي تالي، إذا
+
+217
+00:15:40,620 --> 00:15:42,060
+تكتب بخط نائم
+
+218
+00:15:45,420 --> 00:15:48,780
+لو أنا روحت على أي بلد من بلدان العالم بيحكوا لغة
+
+219
+00:15:48,780 --> 00:15:52,260
+انجليزية، عربية، فرنسية، إيطالية وقولت stuff you
+
+220
+00:15:52,260 --> 00:15:54,960
+look at is ours هذا .. this is a universal name
+
+221
+00:15:54,960 --> 00:16:00,520
+اسم عالم وبنتكلم عن نفس الشخص مابنتهش عن أشخاص
+
+222
+00:16:00,520 --> 00:16:04,480
+مختلفين تمام؟ إذا لما بنقول binomial system هو
+
+223
+00:16:04,480 --> 00:16:10,460
+نظام تسمية طبعا هو نظام قديم واخترا لتسمية
+
+224
+00:16:10,460 --> 00:16:14,220
+النباتات والحيوانات مثلا الفول اسمه Fischia Fava
+
+225
+00:16:14,930 --> 00:16:18,470
+الاليوم ستيفوم و الاليوم سيبا الاليوم ستيفوم أعتقد
+
+226
+00:16:18,470 --> 00:16:22,610
+البصل و الاليوم سيبا .. ستيفوم اللي هو الثوم موزا
+
+227
+00:16:22,610 --> 00:16:26,690
+سابنتوم اللي هو الموز اعتسانونة اللي هو الجشطة هذه
+
+228
+00:16:26,690 --> 00:16:31,630
+أسماء برضه للنباتات يعني فالبكتيريا نفس الشيء
+
+229
+00:16:31,630 --> 00:16:33,890
+Staphylococcus aureus, Escherichia coli,
+
+230
+00:16:34,170 --> 00:16:38,550
+Salmonella typhi إذن هذا خدوا بالكم في رسائل
+
+231
+00:16:38,550 --> 00:16:41,310
+ماليستير مناقشات الطلبة تعود ماليستير في الميكروبط
+
+232
+00:16:41,310 --> 00:16:45,570
+أو أبحاث تخرج ال .. ال .. ال .. بكريوز لو وجدالاسم
+
+233
+00:16:45,570 --> 00:16:49,230
+اللي عليه مكتوب بطريقة خاطئة بيسيبوا كل الموضوع و
+
+234
+00:16:49,230 --> 00:16:51,890
+يمسكوا فيها ليه كتبنا .. فبالتالي اللي يتعلم من
+
+235
+00:16:51,890 --> 00:16:55,230
+الآن و احنا بنكتب يكتبه بطريقة الصحيحة عارف الأول
+
+236
+00:16:55,230 --> 00:16:59,030
+capital من ال genus و ال .. ال specific و ان هو
+
+237
+00:16:59,030 --> 00:17:06,690
+اسمه ال
+
+238
+00:17:06,690 --> 00:17:10,990
+general الموضوع الأصعب و الأعقدفي هذه المحاضرة
+
+239
+00:17:10,990 --> 00:17:12,310
+اللي هو bacteria taxonomy او bacteria
+
+240
+00:17:12,310 --> 00:17:16,850
+classification هو كلهم تقريبا بيعطوا نفس الاتحادات
+
+241
+00:17:16,850 --> 00:17:21,130
+وعودة الى المكتبة نذكركم بالمكتبة مكتبات اللى
+
+242
+00:17:21,130 --> 00:17:26,790
+بتروح عليها تستيير الكتب تم التصنيف الكتب الى اظن
+
+243
+00:17:26,790 --> 00:17:30,710
+التصنيفين انكم باللغة الانجليزية و باللغة العربي
+
+244
+00:17:30,710 --> 00:17:34,270
+روحنا على اللغة الانجليزية وجينا ان في تصنيفات
+
+245
+00:17:34,270 --> 00:17:40,510
+تانية في مراجعغير قابلة الاستعارة و مراجعة قابلة
+
+246
+00:17:40,510 --> 00:17:44,650
+الاستعارة فى عندنا تحت الكل منهما فى science
+
+247
+00:17:44,650 --> 00:17:47,970
+تصنيفات subject فى ال science فى ال chemistry فى
+
+248
+00:17:47,970 --> 00:17:51,850
+ال physics فى كده مظبوط تصنيفات اذا وردنا اكتر من
+
+249
+00:17:51,850 --> 00:17:56,170
+تصنيف او اكتر من معيار يستخدم فى التصنيف فما هي
+
+250
+00:17:56,170 --> 00:18:00,130
+المعايير المستخدمة فى التصنيف فى علم ال
+
+251
+00:18:00,130 --> 00:18:04,330
+microbiology واحد لـ Gramstein فاكرين كنا Gram
+
+252
+00:18:04,330 --> 00:18:06,610
+positive و Gram negative بعد كده كانت نقطة كتير
+
+253
+00:18:06,610 --> 00:18:11,470
+معناال percentage of GC اعتقد كل كنت تعرفه لجوانين
+
+254
+00:18:11,470 --> 00:18:15,870
+سايتوسين والادينين ثايمين درجة القرابة للميكروبات
+
+255
+00:18:15,870 --> 00:18:21,430
+يمكن تحديدها من نسبة التشابه ما بين ال GC content
+
+256
+00:18:21,430 --> 00:18:24,910
+خمسين في المية ستين في المية سبعين في المية ال GC
+
+257
+00:18:24,910 --> 00:18:29,050
+content بيكون جراب لبعض ال two organism تمام؟ إذا
+
+258
+00:18:29,050 --> 00:18:32,690
+كان واحد fifty percent ال GC content وواحد twenty
+
+259
+00:18:32,690 --> 00:18:36,840
+percent دول المعاناة دمية متباعدين عن بعضgrowth
+
+260
+00:18:36,840 --> 00:18:41,580
+temperature شفنا بعض الميكروبات تنمو على low
+
+261
+00:18:41,580 --> 00:18:46,660
+-cyclophil و mesophil و ثرموخيه ability to form
+
+262
+00:18:46,660 --> 00:18:50,700
+heat stable، جونا spore، جونا spore former و non
+
+263
+00:18:50,700 --> 00:18:54,040
+-spore former زي البصيلة و الكريسترديوم، هدولة
+
+264
+00:18:54,040 --> 00:18:57,120
+اندو-spore former وباجين non-indu-spore former
+
+265
+00:18:58,050 --> 00:19:02,530
+الإلكترون acceptors for respiration، شوفنا
+
+266
+00:19:02,530 --> 00:19:04,890
+الأكسجين في ال aerobic respiration، شوفنا ال
+
+267
+00:19:04,890 --> 00:19:08,410
+inorganic compounds في ال anaerobic respiration،
+
+268
+00:19:08,410 --> 00:19:12,030
+شوفنا ال fermentation فيها، organic compound كفانة
+
+269
+00:19:12,030 --> 00:19:15,590
+إلكترون acceptphotosynthetic ability
+
+270
+00:19:15,590 --> 00:19:17,770
+photosynthetic bacteria و non-photosynthetic
+
+271
+00:19:17,770 --> 00:19:21,210
+bacteria motility motile من الموتالي سا قبل لحظة
+
+272
+00:19:21,210 --> 00:19:23,770
+شفنا ال motile cell shape بسيلاي و لا كوكساي و لا
+
+273
+00:19:23,770 --> 00:19:27,050
+spiral و لا curved بطبعا cell shape تشمل معاها
+
+274
+00:19:27,050 --> 00:19:30,370
+كمان ال arrangement ببعض الخلاقات ability to use
+
+275
+00:19:30,370 --> 00:19:32,970
+various carbon and nitrogen sources أعتقد أنه ممكن
+
+276
+00:19:32,970 --> 00:19:37,070
+كارثة المحاضرة كلها أصلا مصابة على أنه في اختلافات
+
+277
+00:19:37,070 --> 00:19:40,010
+في البكتيريا من قدرتها على استخدام المراكبات
+
+278
+00:19:40,010 --> 00:19:43,660
+المختلفةبالضبط زي المكتبة دخلنا نجينا مجموعة
+
+279
+00:19:43,660 --> 00:19:48,640
+تصنيفات، يعني الكتاب لا يخضع لمعيار واحد، انه
+
+280
+00:19:48,640 --> 00:19:51,940
+انجليزي او عربي، لأ خضع لانجليزي او عربي و انه
+
+281
+00:19:51,940 --> 00:19:54,700
+science ولا health و جوا ال health او ال science
+
+282
+00:19:54,700 --> 00:19:59,360
+خضع لانه chemistry ولا physics ولا .. ولا biology،
+
+283
+00:19:59,360 --> 00:20:02,280
+مظبوط؟ ففي مجموعة كبيرة من المعيار المستخدمة في
+
+284
+00:20:02,280 --> 00:20:06,350
+التصنيفability to use various carbon monoxide
+
+285
+00:20:06,350 --> 00:20:09,430
+sources, special nutritional requirement شوفنا
+
+286
+00:20:09,430 --> 00:20:12,830
+بكتيريا تحتاج إلى x and d factors سميناها fast and
+
+287
+00:20:12,830 --> 00:20:18,010
+fast وبكتيريا ال minimum requirement بتجديها
+
+288
+00:20:18,010 --> 00:20:25,850
+سميناها non-fast and fastنجي الآن لمجلد أو مؤلف
+
+289
+00:20:25,850 --> 00:20:27,890
+مشهور جدا في علم ال microbiology أو في علم
+
+290
+00:20:27,890 --> 00:20:31,710
+البكتيروري بالذات اسمه bergesmanuel of systemic
+
+291
+00:20:31,710 --> 00:20:35,230
+bacteriology bergesmanuel of systemic bacteriology
+
+292
+00:20:35,230 --> 00:20:41,430
+كلمة systemic لها علاقة بالتصنيف خدوا بالكم لأن
+
+293
+00:20:41,430 --> 00:20:45,430
+التصنيف عملية نظامية أساسا عملية نظامية معتمدة على
+
+294
+00:20:45,430 --> 00:20:47,390
+فبعض الحياة بيسموه systemic
+
+295
+00:20:50,760 --> 00:20:55,220
+اللوحات اللي امامنا هدول هنحفظهم؟ لأ مش هنحفظهم طب
+
+296
+00:20:55,220 --> 00:20:58,460
+و ليش بندرسهم؟ مدام انتوا عارفين انتوا يعني مولودة
+
+297
+00:20:58,460 --> 00:21:02,500
+يعني بالتأكيد قالية مبرمجة في أدمغاتكم انه مدام مش
+
+298
+00:21:02,500 --> 00:21:06,300
+في الامتحان بيجيباش للدراسة لأول الدراسة و لكليس
+
+299
+00:21:06,300 --> 00:21:11,420
+الامتحان لأنه تلقائي و احنا بيدرس فيهم هنستفيد
+
+300
+00:21:11,420 --> 00:21:15,760
+للمستقبل فهنلاجح حالنا هنحفظهم لامتحان بس مش
+
+301
+00:21:15,760 --> 00:21:20,330
+امتحان المنتقل طيبالمجسمين احنا في الbergers
+
+302
+00:21:20,330 --> 00:21:24,710
+manual المجموعات الى تلات تلاتين section تلات
+
+303
+00:21:24,710 --> 00:21:29,590
+تلاتين section همور عليهم مرور نتعرف عليهم تعرف
+
+304
+00:21:29,590 --> 00:21:35,230
+سريع و بسيط منهم قد نجف عليه لحظات و البعض الاخر
+
+305
+00:21:35,230 --> 00:21:40,860
+مش هنجف عليه لإن اسمه بيكون وصف لحالهده
+
+306
+00:21:40,860 --> 00:21:45,660
+Spirochetes Spirochetes وبين جثين هي ال gram
+
+307
+00:21:45,660 --> 00:21:50,680
+negative spiral shaped cells خلايا ده شكل لولة
+
+308
+00:21:50,680 --> 00:21:55,080
+with endoflagella أول مرة أتكلم على endoflagella
+
+309
+00:21:55,080 --> 00:22:01,000
+ال endoflagella فاكرين ان كانت ال endoflagella على
+
+310
+00:22:01,000 --> 00:22:08,040
+ال bacteria ظاهرة اما على طرف او طرفين او على
+
+311
+00:22:08,040 --> 00:22:12,620
+الألج في الأخر كانتهي عبارة عن زوائد البكتيرى اللى
+
+312
+00:22:12,620 --> 00:22:15,620
+بتحدث عنها شوية بتختلف احنا قولنا هنا قبل شوية
+
+313
+00:22:15,620 --> 00:22:22,200
+spiral بدنا نضخمها شوية اعتبروها
+
+314
+00:22:22,200 --> 00:22:28,260
+بدون cell wall بدون cell wall هان طالع منها فلاجلة
+
+315
+00:22:28,260 --> 00:22:35,780
+بس بدل ما يطلع برا وبعدين
+
+316
+00:22:35,780 --> 00:22:36,180
+جميل
+
+317
+00:22:43,490 --> 00:22:48,610
+الان صار عندى مباني ال cell membrane و ال cell
+
+318
+00:22:48,610 --> 00:22:55,690
+wall فى عندى خيط marine marine زى ال rubber تمام
+
+319
+00:22:55,690 --> 00:23:00,410
+البكتيريا شكلها لهو اللى بيه لو انا مسكت طرفها
+
+320
+00:23:00,410 --> 00:23:05,630
+منها لهو او هى اخدت قرار اخدت قرار انه بيتحرك تمال
+
+321
+00:23:05,630 --> 00:23:09,970
+twitching twitching يعني بتلف لو تركت المنطقة اللى
+
+322
+00:23:09,970 --> 00:23:14,720
+حاسس فيهاهتروح معاها لإن هو Marina فلما يتحرك حول
+
+323
+00:23:14,720 --> 00:23:18,660
+المحور تبعها، إيش بيصير فيها؟ تتحرك إلى الأمام أو
+
+324
+00:23:18,660 --> 00:23:23,720
+إلى الخلف وبالتالي it moves by rotating itself
+
+325
+00:23:23,720 --> 00:23:28,600
+around its axis نفس المحور تبعها، تتحرك وبتحرك من
+
+326
+00:23:28,600 --> 00:23:32,200
+خلاله، إن هال اللي عنده فلجلة ولا اسم تاني اسمها
+
+327
+00:23:32,200 --> 00:23:35,400
+axial filament
+
+328
+00:23:43,030 --> 00:23:48,010
+إذن الآن بنقدر نقول إنه صار فينا معارفة بمجموعة
+
+329
+00:23:48,010 --> 00:23:50,530
+اسمها Spirocates والـ Spirocates كنا نقولها بقى
+
+330
+00:23:50,530 --> 00:23:53,610
+بيبقى Ground Negative Spiral Organism وقالها Endo
+
+331
+00:23:53,610 --> 00:23:57,310
+Flagellate بتاخدوا مثال عليها؟ خدوا ليكم مثال
+
+332
+00:23:57,310 --> 00:24:04,410
+تريبونيما باليدوم تريبونيما حتمر معانا كمان شوية
+
+333
+00:24:04,410 --> 00:24:08,170
+تريبونيما وبتسبب مرض اسمه مرض الـ syphilis مرض الـ
+
+334
+00:24:08,170 --> 00:24:11,190
+syphilis طيب
+
+335
+00:24:12,380 --> 00:24:18,340
+مرض الـ syphilis المجموعة
+
+336
+00:24:18,340 --> 00:24:24,840
+الرقم اتنين هي عبارة عن aerobic او ميكرو ايروفيليك
+
+337
+00:24:24,840 --> 00:24:30,440
+مقطع
+
+338
+00:24:30,440 --> 00:24:35,140
+الهيليكل فيبريويد فيبريويد يعني شكلها زي الفيبريو
+
+339
+00:24:35,140 --> 00:24:39,000
+أو هيليكل يعني في واحدة من اتنتين يا اما هيليكل يا
+
+340
+00:24:39,000 --> 00:24:44,480
+اما فيبريويدground negative bacteria رقم تلاتة non
+
+341
+00:24:44,480 --> 00:24:49,100
+motile or rarely motile تشوفوا بعض الأحيان التصنيف
+
+342
+00:24:49,740 --> 00:24:54,060
+يعني في الكتب هذا Biology بيولوجي طب لو أتينا واحد
+
+343
+00:24:54,060 --> 00:24:57,440
+في ال physiology هو ممكن يكون health وممكن يكون
+
+344
+00:24:57,440 --> 00:25:00,420
+biology فممكن أحط نسخة في ال biology و أحط نسخة
+
+345
+00:25:00,420 --> 00:25:08,700
+هنا في ال physiology فممكن أحط
+
+346
+00:25:08,700 --> 00:25:09,300
+فممكن أحط نسخة في ال biology و أحط نسخة هنا في ال
+
+347
+00:25:09,300 --> 00:25:09,700
+physiology فممكن أحط نسخة في ال biology و أحط نسخة
+
+348
+00:25:09,700 --> 00:25:10,060
+هنا في ال physiology فممكن أحط نسخة في ال biology
+
+349
+00:25:10,060 --> 00:25:11,740
+و أحط نسخة هنا في ال physiology فممكن أحط نسخة في
+
+350
+00:25:11,740 --> 00:25:14,600
+ال biology و أحط نسخة هنا في ال physiology فممكن
+
+351
+00:25:14,600 --> 00:25:17,560
+أحط نسخة في ال biology و أحط نسخة هنا في ال
+
+352
+00:25:17,560 --> 00:25:20,340
+physiology فممكن أحط نسخة في ال biologyتصنيف الكتب
+
+353
+00:25:20,340 --> 00:25:23,380
+قمر سهل، يعني حدث كبير أو معروف أو واضح بالنسبة
+
+354
+00:25:23,380 --> 00:25:26,340
+لنا، بس بالنسبة للبكتيريا لإن معلوماتنا بتزيد و
+
+355
+00:25:26,340 --> 00:25:31,880
+بتتغير بستمرة فحتلاجوا تغيير في التصنيفات بستمرة،
+
+356
+00:25:31,880 --> 00:25:37,600
+أعطيكم مثل هذافيه كانت بكتيريا اسمها Enterobacter
+
+357
+00:25:37,600 --> 00:25:42,960
+Sakazaki وبكتيريا بانتباهها جديدة يعني عملنا عليها
+
+358
+00:25:42,960 --> 00:25:46,380
+دراسة في الجامعة هنا واحنا مكتب في ال paper تبعت
+
+359
+00:25:46,380 --> 00:25:49,780
+الموضوع لجينا انه صار فيه تصنيفات اتغير الاسم صار
+
+360
+00:25:49,780 --> 00:25:53,540
+اسمها Chronobacter Sakazaki مين اللي اتغير؟
+
+361
+00:25:57,850 --> 00:26:02,450
+اليرسينيا بيستس كان اسمها pasturella biestes
+
+362
+00:26:02,450 --> 00:26:09,490
+فغالبا ممكن تكون التغيير فقط نقل البكتيريا من
+
+363
+00:26:09,490 --> 00:26:13,950
+genus إلى genus طب و احنا ايش كنا؟ كنا مش عارفين؟
+
+364
+00:26:13,950 --> 00:26:16,430
+لما حطينا البكتيريا ده في genus معين كنا مش
+
+365
+00:26:16,430 --> 00:26:21,840
+عارفين؟ نعم كنا مش عارفينمعلوماتنا كانت قليلة لما
+
+366
+00:26:21,840 --> 00:26:27,040
+زادت معلوماتنا اثبتت ان هذا ال microbe لا يستحق ان
+
+367
+00:26:27,040 --> 00:26:31,040
+يكون في هذا التصنيف وجب ان ينقل اما اسم جديد او ان
+
+368
+00:26:31,040 --> 00:26:35,680
+ينقل حتى لgenus اخر فعشان اقولكم في تكسونومي في
+
+369
+00:26:35,680 --> 00:26:40,720
+نوع من الصعوبة وفي نفس الوجد في انا تغيرات مستمرة
+
+370
+00:26:40,720 --> 00:26:43,520
+في هذا المشكلة
+
+371
+00:26:46,620 --> 00:26:51,560
+Aerobic rods and cocci, facultatively anaerobic
+
+372
+00:26:51,560 --> 00:26:57,480
+gram negative rods, anaerobic
+
+373
+00:26:57,480 --> 00:27:01,320
+gram negative straight curved and helical rods
+
+374
+00:27:06,470 --> 00:27:10,190
+dissimilatory sulfate أو اسم الأشهر لها sulfur
+
+375
+00:27:10,190 --> 00:27:15,030
+-reducing بكتيريا sulfur-reducing واضح من اسمها إن
+
+376
+00:27:15,030 --> 00:27:19,510
+هذه البكتيريا تتغذى على المراكبات الكبريه أنا
+
+377
+00:27:19,510 --> 00:27:23,150
+aerobic ground negative cocci دا ريكيزياس و
+
+378
+00:27:23,150 --> 00:27:26,070
+كلاميدياس هذا الأول مرة تسمعوا فيهم شيلوا ال S من
+
+379
+00:27:26,070 --> 00:27:30,590
+كل واحدة منهم و هتعطيهم خط كلاميديا و ريكيزيا
+
+380
+00:27:30,590 --> 00:27:37,540
+كلاميديا و ريكيزيا ايش منام دولة؟هدولة the only
+
+381
+00:27:37,540 --> 00:27:41,640
+بالنسبالنا المعروفين the only obligate
+
+382
+00:27:41,640 --> 00:27:46,760
+intracellular bacteria، إيش يعني؟ هي البكتيريا
+
+383
+00:27:46,760 --> 00:27:51,640
+الوحيدة المطفلة طفرا إشفاريا من البكتيريا، بتذكرنا
+
+384
+00:27:51,640 --> 00:27:55,440
+من مين المصطلح؟ من ال viruses، ال viruses هم
+
+385
+00:27:55,440 --> 00:27:59,600
+obligate intracellular virusesوبالتالي هدول
+
+386
+00:27:59,600 --> 00:28:04,200
+الميكروبات أقرب ما يكون في العلاقة ما بينهم بين
+
+387
+00:28:04,200 --> 00:28:06,900
+الخلايا إلى الفيروسات لأنهم obligate intracellular
+
+388
+00:28:06,900 --> 00:28:10,840
+برزات أنا للأسف مش مكتوب كلمة obligate هي الأصل
+
+389
+00:28:10,840 --> 00:28:18,020
+إنه إن حطنا obligate intracellular برزات لش
+
+390
+00:28:18,020 --> 00:28:24,720
+أنا مصرح أحط obligate لإن هناك facultativeنعم مثلا
+
+391
+00:28:24,720 --> 00:28:28,040
+زي السيرمونيلا ممكن تعيش معايشة حرة و ممكن تعمل
+
+392
+00:28:28,040 --> 00:28:33,270
+intra .. بصرا .. intra cellular برضهطبعا ال
+
+393
+00:28:33,270 --> 00:28:37,230
+rickettsia و ال chlamydia ميكروبات مهمة بتسبب
+
+394
+00:28:37,230 --> 00:28:41,210
+أمراض، قيلنا في منها ال rickettsia بتسبب أمراض في
+
+395
+00:28:41,210 --> 00:28:45,310
+.. في فلسطين اللي هو homo typhus مش ال typhoid،
+
+396
+00:28:45,310 --> 00:28:50,910
+homo typhus و بينتقل عن طريق الحشرات و موجود أنه
+
+397
+00:28:50,910 --> 00:28:54,550
+في .. في .. في غزة بيتشر في شهر سبعة إلى شهر تسعة
+
+398
+00:28:54,550 --> 00:28:57,010
+تقريبا أو شهر عشر يعني .. يعني الفترة الزمنية اللي
+
+399
+00:28:57,010 --> 00:29:02,770
+احنا موجودين فيها الآنالقلميديا معروف عنها فاكرين
+
+400
+00:29:02,770 --> 00:29:07,440
+ميسيليا جونورية؟ ميسيليا جونورية ايش بيقول عنها؟لأ
+
+401
+00:29:07,440 --> 00:29:10,440
+نفسينا كنا نري عنها كنا من أشهر .. من أشهر
+
+402
+00:29:10,440 --> 00:29:12,700
+الميكروفات المسببة لل STDs اللي هي sexual
+
+403
+00:29:12,700 --> 00:29:16,220
+transmitted diseases نتقل عن طريق الجنس كنا هي ..
+
+404
+00:29:16,220 --> 00:29:19,960
+هي رقم اتنين مين رقم واحد؟ رقم واحد الكليكيا رقم
+
+405
+00:29:19,960 --> 00:29:24,860
+واحد الكليكيا طبعا تبتدأ الأمراض أخرى غير ال .. ال
+
+406
+00:29:24,860 --> 00:29:27,200
+.. ال .. الأمراض الجنسية ممكن تاما الحادثة اسمها
+
+407
+00:29:27,200 --> 00:29:31,500
+Trachoma Trachoma بالعربي Trachoma و Trachoma هو
+
+408
+00:29:31,500 --> 00:29:36,840
+ما بيصيب العين و قد يؤدي إلى ال blindness في قرىفي
+
+409
+00:29:36,840 --> 00:29:41,880
+أفريقيا يمكن أن يكون نص السكان فقدوا البصر لأن
+
+410
+00:29:41,880 --> 00:29:47,100
+عندهم الكلاميديا متشرقة بشكل كبير، يدعي ركيزيا
+
+411
+00:29:47,100 --> 00:29:53,500
+والكلاميديا كاسمها مهمين جدا في عالم الطبالميكو
+
+412
+00:29:53,500 --> 00:29:56,880
+بلازمات ما تقولوليش ماسمعتش فيها هذه، ماسمعت فيها
+
+413
+00:29:56,880 --> 00:30:02,860
+هي الأصغر، دي هي الأصغر بكتير وهي wall-less أو
+
+414
+00:30:02,860 --> 00:30:08,720
+lacks a defined cell wall، فيها إنها حاسمة
+
+415
+00:30:08,720 --> 00:30:16,390
+endosymbiosisو Endosymbionts يعني بكتيريا تعيش
+
+416
+00:30:16,390 --> 00:30:20,990
+في خلايا كائنات أخرى زي مثلا البكتيريا العقد
+
+417
+00:30:20,990 --> 00:30:25,470
+الجدرية اللي بنشوفها في نباتات زي الفول الرقم
+
+418
+00:30:25,470 --> 00:30:28,030
+اتناشر اللي هم ال gram-bostococci مشهورة هذه زي
+
+419
+00:30:28,030 --> 00:30:31,350
+الستفليكوكاس و الستربتوكوكاس ال endospore-forming
+
+420
+00:30:31,350 --> 00:30:35,170
+بكتيريا تبعت ال gram-bostobacilli زي البسيلس و
+
+421
+00:30:35,170 --> 00:30:39,940
+الكلسترديوم و طبعا مجموعتين مهمين جداregular non
+
+422
+00:30:39,940 --> 00:30:44,860
+-sporing gram-bostef-bacilli كلمة regular يعني
+
+423
+00:30:44,860 --> 00:30:49,200
+شكلها منتظم في irregular شكلها غير منتظم
+
+424
+00:30:49,200 --> 00:30:55,940
+الميكوبكتيريا الميكوبكتيريا إلها إسمين من أشهر
+
+425
+00:30:55,940 --> 00:30:59,160
+الميكروبرد على كوكب الأرضmycobacterium
+
+426
+00:30:59,160 --> 00:31:03,060
+tuberculosis و بتسبب مرض السل الرئوي او الدرن
+
+427
+00:31:03,060 --> 00:31:07,900
+mycobacterium tuberculosis فلسطين او غزة بالذات
+
+428
+00:31:07,900 --> 00:31:13,900
+بتعاني سنويا من خمستاش تقريبا خمستاش حالة سنويا
+
+429
+00:31:13,900 --> 00:31:19,560
+فقط يعني العيب منخفض لان في فلسطين العيب منخفض لان
+
+430
+00:31:19,560 --> 00:31:25,040
+ان بتعرفوا اللي يسموها اللقاح التخريصة اللي في
+
+431
+00:31:25,040 --> 00:31:33,520
+الكتفاسم اللقاح BCG احنا الان في قطاع غزة الارقام
+
+432
+00:31:33,520 --> 00:31:38,400
+تبعتنا تضاهي او افضل من بعض الدول الأوروبية بسبب
+
+433
+00:31:38,400 --> 00:31:41,380
+ان احنا لسه ما زلنا بناخد اللقاح هم بطلوا ياخدوا
+
+434
+00:31:41,380 --> 00:31:45,720
+اللقاحليه بطل ياخدوا اللقاح و احنا ناخدوا اللقاح؟
+
+435
+00:31:45,720 --> 00:31:50,900
+هم بطروا لإنه لقوا نسبة الإصابة من التطعيم نفسه
+
+436
+00:31:50,900 --> 00:31:53,480
+يعني الناس اللي بتنصاب بعد ما بتطعمه يعني من
+
+437
+00:31:53,480 --> 00:31:58,140
+التطعيم سابق تطعيم أعلى من اللي بيكسبوا الإصابة من
+
+438
+00:31:58,140 --> 00:32:03,240
+natural sources فجاله بنواجه طبعا انه صار تحسن في
+
+439
+00:32:03,240 --> 00:32:07,120
+الصحة العامة و مستوى التغذية في .. في .. في
+
+440
+00:32:07,120 --> 00:32:12,280
+البلدان الأوروبية فهذا المرة تقريبا شبه ان عدمأحنا
+
+441
+00:32:12,280 --> 00:32:18,540
+لسه فيه مشاكل تغذية وبتالي WHO قالت خلّي التطعيم
+
+442
+00:32:18,540 --> 00:32:22,720
+جاري وبتالي هو جوفرنا حماية ونسبة من الخمس عشر حال
+
+443
+00:32:22,720 --> 00:32:24,980
+اللي بحكيلكوا عنها سنويا نسبة بتكون نجلي عن
+
+444
+00:32:24,980 --> 00:32:30,800
+الفعالي عن الفاكسين بتصيب الأطفال اللي بتطعمه
+
+445
+00:32:33,500 --> 00:32:37,860
+البكتيريا اللى اعتقدنا انه فى أوروبا وامريكا قضية
+
+446
+00:32:37,860 --> 00:32:43,400
+عليها ظهرت مرة أخرى وظهرت بشكل خطير جدا وهو شكل
+
+447
+00:32:43,400 --> 00:32:47,760
+بنسميه احنا multiple drug resistant يعني سلالات
+
+448
+00:32:47,760 --> 00:32:54,160
+مقاومة للعديد من المضادات الحيوية فعادت عودة يعني
+
+449
+00:32:54,160 --> 00:32:59,140
+أشرس من ذي قبل فهو بالتالي الان فى جهود كبيرة لحل
+
+450
+00:32:59,140 --> 00:33:03,370
+الإشكالات المتعلقة بهذا ال microطيب ال microbe
+
+451
+00:33:03,370 --> 00:33:06,670
+التاني الاسم التاني و انه mycobacterium
+
+452
+00:33:06,670 --> 00:33:09,630
+tuberculosis اللى هى اللى هتحدثنا عنها التانى
+
+453
+00:33:09,630 --> 00:33:13,510
+mycobacterium librae و librae اللى هو من جامع كلمة
+
+454
+00:33:13,510 --> 00:33:19,470
+librosi، librosi مار معاكم جو دام؟ جو دامالرسول
+
+455
+00:33:19,470 --> 00:33:23,390
+صلى الله عليه و سلم بيقول في حادثة الشريف لا عدوة
+
+456
+00:33:23,390 --> 00:33:29,250
+ولا طيرة ولا همه ولا صفر وفره من المجذومي فره كمن
+
+457
+00:33:29,250 --> 00:33:35,050
+الأسد المجذوم هو الانسان المصاب بالجدام و الجدام
+
+458
+00:33:35,050 --> 00:33:39,090
+هو اللي بتسببه البكتيريا اللي اسمها Mycobacterium
+
+459
+00:33:39,090 --> 00:33:47,890
+Libra الجدام مشهور أكتر وين؟في الهند في باكستان في
+
+460
+00:33:47,890 --> 00:33:53,110
+بنجليدش في الدول الأسيوية الجنوب الشرق أسيا هذه
+
+461
+00:33:53,110 --> 00:33:56,410
+بلد بلدها مشهورة بالجودان الجزيرة العربية كان فيها
+
+462
+00:33:56,410 --> 00:33:59,870
+جودان فلسطين أنا شوفت حالة واحدة فقط فيها جودان
+
+463
+00:33:59,870 --> 00:34:02,390
+مجاهدة من برا يعني مش من هنا أساسا
+
+464
+00:34:07,700 --> 00:34:10,720
+لحد الآن بكتيريا الريودام لم نستطع تنميتها، لم
+
+465
+00:34:10,720 --> 00:34:15,100
+نستطع تنميتها، وتوصف ال micro bacteria الصينفين
+
+466
+00:34:15,100 --> 00:34:19,810
+اللي أجابتهم أنا ب slow growing bacteriaفاكرين ال
+
+467
+00:34:19,810 --> 00:34:23,250
+equalize؟ فاكرين ال equalize؟ فاكرين ال equalize؟
+
+468
+00:34:23,250 --> 00:34:23,750
+فاكرين ال equalize؟ فاكرين ال equalize؟ فاكرين ال
+
+469
+00:34:23,750 --> 00:34:24,050
+equalize؟ فاكرين ال equalize؟ فاكرين ال equalize؟
+
+470
+00:34:24,050 --> 00:34:26,110
+فاكرين ال equalize؟ فاكرين ال equalize؟ فاكرين ال
+
+471
+00:34:26,110 --> 00:34:28,530
+equalize؟ فاكرين ال equalize؟ فاكرين ال equalize؟
+
+472
+00:34:28,530 --> 00:34:28,950
+فاكرين ال equalize؟ فاكرين ال equalize؟ فاكرين ال
+
+473
+00:34:28,950 --> 00:34:29,030
+فاكرين ال equalize؟ فاكرين ال equalize؟ فاكرين ال
+
+474
+00:34:29,030 --> 00:34:31,350
+فاكرين ال equalize؟ فاكرين ال equalize؟ فاكرين ال
+
+475
+00:34:31,350 --> 00:34:37,950
+equalize؟ فاكرين ال equalize؟
+
+476
+00:34:37,950 --> 00:34:40,390
+فاكرين ال equalize؟
+
+477
+00:34:41,900 --> 00:34:45,900
+Cardioform وهي acid-fast بكتيريا، إيش يعني acid
+
+478
+00:34:45,900 --> 00:34:49,060
+-fast بكتيريا؟ إذا فاكرين لما تحدثت عن ال gram
+
+479
+00:34:49,060 --> 00:34:52,760
+-stain بالظبط، لما تحدثنا عن ال gram-stain، كنا
+
+480
+00:34:52,760 --> 00:34:55,960
+بيقسم البكتيريا ل gram بُسط و gram نيجر، الان
+
+481
+00:34:55,960 --> 00:35:00,680
+أكبرتوا شويه أنتوا، قولالكوا أنه لا يوجد أو أو
+
+482
+00:35:00,680 --> 00:35:03,380
+بالأحرى ال gram-stain لا يستطيع سببها تكون
+
+483
+00:35:03,380 --> 00:35:07,520
+البكتيريا، ليش؟ في نوع من البكتيرياعنده طبقة من ال
+
+484
+00:35:07,520 --> 00:35:10,860
+wax celibate يصعب صدارة البكتيريا عشان نصبغها
+
+485
+00:35:10,860 --> 00:35:15,320
+بنسخن البكتيريا بنحط الصبغة و بنسخن البكتيريا تدخل
+
+486
+00:35:15,320 --> 00:35:18,580
+الصبغة لما نشيل الصبغة نعمل ال decolourization
+
+487
+00:35:18,580 --> 00:35:24,960
+بيكون في صعوبة خالص حتى لو ضفنا مزيد لللون قوي
+
+488
+00:35:24,960 --> 00:35:27,860
+فاكرين احنا نشيل الصبغة داخل الجراز التاني اثانول
+
+489
+00:35:27,860 --> 00:35:36,400
+هنت بستخدم خلطة من الاثانول و ال sulfuric acidو
+
+490
+00:35:36,400 --> 00:35:40,740
+الحمض و الحمض المعروف عنه انه بيزيل اللون اكتر من
+
+491
+00:35:40,740 --> 00:35:45,000
+الكحول فاسمع الجملة دي، اسمع الجملة و بعدين نفسرها
+
+492
+00:35:45,000 --> 00:35:48,400
+قطع قطع عشان نقولكوا ايش معناه acid fast bacteria
+
+493
+00:35:56,180 --> 00:36:00,360
+but once stained they are difficult to decolorize
+
+494
+00:36:00,360 --> 00:36:04,400
+even in the presence of strong acid قطع الأولى
+
+495
+00:36:04,400 --> 00:36:07,800
+بتقول صعبة الصبانة لكن ماكنها مستحيلة صبانها
+
+496
+00:36:07,800 --> 00:36:12,350
+بالتسخينBut once stained, it is difficult to
+
+497
+00:36:12,350 --> 00:36:19,030
+decolorize، حتى في وجود حامض قوى، اسمها الجملة
+
+498
+00:36:19,030 --> 00:36:24,190
+اللي بتقول acid fast بكتيريا، بكتيريا مقاومة
+
+499
+00:36:24,190 --> 00:36:31,810
+للحمض، fast معناها صايم، fasting معناها سياسي،
+
+500
+00:36:36,790 --> 00:36:39,790
+فبالتالي بنقول اللي هو الوصف الدقيق اللي باللغة
+
+501
+00:36:39,790 --> 00:36:44,510
+العربية الصلغ المقاوم للحمض بمعنى المقاوم للإزالة
+
+502
+00:36:44,510 --> 00:36:49,790
+بالحمض طبعا؟ فهناك أنواع من البكتيريا إذا صورناها
+
+503
+00:36:49,790 --> 00:36:53,090
+ب acid-fast-stain هي الصواب إزالة فنسميها acid
+
+504
+00:36:53,090 --> 00:36:56,530
+-fast-bacteria acid-fast-bacteria بينما اللي بيزال
+
+505
+00:36:56,530 --> 00:36:59,970
+منها اللون العادى يعني نسميها non acid-fast
+
+506
+00:36:59,970 --> 00:37:02,850
+-bacteria بينما لو كنا نقول gram positive و gram
+
+507
+00:37:02,850 --> 00:37:05,230
+negative بنقول acid-fast و non acid-fast
+
+508
+00:37:09,000 --> 00:37:13,020
+انوكسجينك فوتو تروفيك بكتيريا، انوكسجينك يعني في
+
+509
+00:37:13,020 --> 00:37:18,560
+غياب الأكسجين، و بتعمل photosynthesis أكسجينك فوتو
+
+510
+00:37:18,560 --> 00:37:23,580
+سنتيك بكتيريا، زي الـ cyanobacteria، لو حطينا ميه
+
+511
+00:37:23,580 --> 00:37:28,440
+في مرتبال، plastic
+
+512
+00:37:36,290 --> 00:37:40,850
+اللون الأخضر هذا عبارة عن انسيانو بكتيريا، انسيانو
+
+513
+00:37:40,850 --> 00:37:46,170
+بكتيريا هو بعض الطحالب، عشان هي التنكات المي اللي
+
+514
+00:37:46,170 --> 00:37:49,690
+فوق اللي بتكون في الإضاءة، إيش بيكون اللون هذا؟
+
+515
+00:37:54,010 --> 00:37:58,530
+لكن غير منفذة للضوء، غير منفذة يعني بتكون معتمة،
+
+516
+00:37:58,530 --> 00:38:01,970
+بتكون معتمة، مش شفافة، مافي لون أبيض اللي بيكون
+
+517
+00:38:01,970 --> 00:38:06,710
+شفافة، بيبقى لون أبيض معتم، أسود معتم، أخضر معتم،
+
+518
+00:38:06,710 --> 00:38:11,530
+أزرق معتم، المهم إنه ماينفذش من الضوء Aerobic
+
+519
+00:38:11,530 --> 00:38:14,430
+chemolithropic bacteria and associated organisms
+
+520
+00:38:14,430 --> 00:38:17,130
+كلمة associated organisms معناها بحكي إنه هذا
+
+521
+00:38:17,130 --> 00:38:23,750
+عبارة عن مجموعة والحقنا بيها مجموعة أخرىرقم 21
+
+522
+00:38:23,750 --> 00:38:28,670
+غريبة budding and or appendaged bacteria يعني
+
+523
+00:38:28,670 --> 00:38:34,670
+بكتيريا بيكون لها براهم أو لها زواقات لها كلمة
+
+524
+00:38:34,670 --> 00:38:40,330
+appendaged معناها زواقات sheathed bacteria بكتيريا
+
+525
+00:38:40,330 --> 00:38:49,900
+لها أغلفة أو لها طبقات رقم 23 فيها حاجة حلوةnon
+
+526
+00:38:49,900 --> 00:38:54,040
+-photosynthetic، non-fruiting،
+
+527
+00:38:54,040 --> 00:38:59,620
+الكلمة fruiting body مصطلحة صحيح بنجيب واحنا من
+
+528
+00:38:59,620 --> 00:39:04,180
+وين من الفطريات، انتوا بتعرفوا الفطر المشروم،
+
+529
+00:39:04,180 --> 00:39:06,920
+المشروم اللي احنا بنشوفه هذا ال fruiting body، هذا
+
+530
+00:39:06,920 --> 00:39:10,260
+ال fruiting body، لكن على فكرة لو انتوا شفتوا
+
+531
+00:39:10,260 --> 00:39:15,940
+المشروم هو طالع في الأرض في فصل الشتاءلما ت .. ت
+
+532
+00:39:15,940 --> 00:39:19,400
+.. تخلع المشروم هذا، ابقي عصاية صغيرة أو عودة
+
+533
+00:39:19,400 --> 00:39:22,300
+صغيرة، حاولوا تحركوا التربة اللي حوالها، هتلاقوا
+
+534
+00:39:22,300 --> 00:39:25,860
+آلاف، ملايين من خيوط البيضاء في المنطقة، هذا هو
+
+535
+00:39:25,860 --> 00:39:29,480
+الفطر، الجزء اللي طولي هذا اسمه ال floating body،
+
+536
+00:39:29,480 --> 00:39:32,260
+في بعض أنواع من البكتيريا عاملها floating body و
+
+537
+00:39:32,260 --> 00:39:35,520
+من بعض الآخر اللي مش هو المهم، أنا عندي كلمة
+
+538
+00:39:35,520 --> 00:39:38,870
+gliding هي المهمةالبكتيريا احنا قلناها قبل شوية
+
+539
+00:39:38,870 --> 00:39:43,530
+ايه ما متحرك ايه ما غير متحرك واتفجنا اول محاضرات
+
+540
+00:39:43,530 --> 00:39:47,870
+انه متحركة ب flagella واليوم يعني اه قلنالكوا انه
+
+541
+00:39:47,870 --> 00:39:51,390
+لا يا ابنات فى بكتيريا متحركة بس ب endoflagella
+
+542
+00:39:51,390 --> 00:39:54,550
+طبعا يعني كل مرة كنا بنكبر شوية و بنكتشف ان
+
+543
+00:39:54,550 --> 00:40:00,270
+الحقيقة مش مطلقة ان المعلومات تبعتنا مش مطلقةيعني
+
+544
+00:40:00,270 --> 00:40:02,250
+اما flagella يبقى بدون flagella، لأ لجينا
+
+545
+00:40:02,250 --> 00:40:05,430
+endoflagella، بالمرحلة لجينا بكتيريا متحركة
+
+546
+00:40:05,430 --> 00:40:11,310
+ومتزحقة، flying، كيف تزحق flying؟ بتفرز مادة، بس
+
+547
+00:40:11,310 --> 00:40:15,870
+ورضه حتى فرازة المادة، فرازة المادة، تقصد لزجة، بس
+
+548
+00:40:15,870 --> 00:40:21,070
+مش هيك الفكرة كمان، فكرة أعقد منها، تخيل رأي
+
+549
+00:40:21,070 --> 00:40:25,180
+البكتيريا بهذا الشكل؟البكتيريا قررت تروح على
+
+550
+00:40:25,180 --> 00:40:28,200
+اتجاهها وتتحرك على اتجاهها اللي بتروحه هي مفر زي
+
+551
+00:40:28,200 --> 00:40:37,440
+مراكبات .. مراكبات .. سالبة الشحن بكميات كبيرة في
+
+552
+00:40:37,440 --> 00:40:41,060
+المنطقة اللي هي مورودة فيها الآن، تمام؟ هي عالق
+
+553
+00:40:41,060 --> 00:40:48,190
+على الوسط تبعها، كويس؟وفي نفس الوقت بتروح تنتج
+
+554
+00:40:48,190 --> 00:40:52,790
+مراكبات و بتخزنهم في مؤخرة البكتيريا .. مؤخرة أو
+
+555
+00:40:52,790 --> 00:40:57,930
+مقدمة .. ممكن تصير على عكسها .. الان negatively
+
+556
+00:40:57,930 --> 00:41:01,370
+charged كمية كبيرة من المراكبات و negatively
+
+557
+00:41:01,370 --> 00:41:04,010
+charged مراكبات ايضا مولدة داخل الخليج .. ايش
+
+558
+00:41:04,010 --> 00:41:09,310
+هاسير؟ revolutionهو كمان هتزوج البكتيريا اللى
+
+559
+00:41:09,310 --> 00:41:13,610
+أمامك فتحركت ليست بفعل فلاجلة وانا بفعل ال
+
+560
+00:41:13,610 --> 00:41:18,950
+revolution اللى هو التنافر ما بين ال negatively
+
+561
+00:41:18,950 --> 00:41:22,750
+charged يعني هى اللى أنطلتها وخزنتها فى المنطقة
+
+562
+00:41:22,750 --> 00:41:26,130
+وخزنها فى نفس الشىء عندها عشان يصير فى تنافر،
+
+563
+00:41:26,130 --> 00:41:29,590
+plasma gliding البكتيريا طبعا العملية تتسهل أكتر
+
+564
+00:41:29,590 --> 00:41:32,010
+لورود mucus أو مادة ميوكويد
+
+565
+00:41:34,870 --> 00:41:38,490
+طبعا فيه قولنا gliding و فيه non-floating gliding
+
+566
+00:41:38,490 --> 00:41:41,830
+و فيه gliding floating bacteria ال archaeobacteria
+
+567
+00:41:41,830 --> 00:41:44,450
+أظن أحكيت عنها أنا في أول المحاضرة اللي قلت هي
+
+568
+00:41:44,450 --> 00:41:47,410
+البكتيريا المسمى بالبكتيريا القديمة
+
+569
+00:41:51,250 --> 00:41:54,970
+الـ extremophil و ماعرف احنا ميزناها عن البكتيريا
+
+570
+00:41:54,970 --> 00:41:59,790
+العادية ولا ميزناش بعدم وجود ببتيدوجلايكان حقيقي
+
+571
+00:41:59,790 --> 00:42:03,990
+يعني فيها ببتيدوجلايكان بس مش حقيقي بمعنى انه مش
+
+572
+00:42:03,990 --> 00:42:07,850
+مكوّن من نام و ناج فاكرين نام و ناج؟ in acetylamic
+
+573
+00:42:07,850 --> 00:42:11,810
+acid و in acetylglucose amine هذه كلها in
+
+574
+00:42:11,810 --> 00:42:15,230
+acetylglucose amine يعني مافيش .. مافيش واحدات ..
+
+575
+00:42:15,230 --> 00:42:19,960
+واحدات متعاقبةفيه ايضا انه cardioform أكتينوميسيتس
+
+576
+00:42:19,960 --> 00:42:22,860
+الأكتينوميسيتس أنا مرة ذكرتلكوا إيها و قولتلكوا إن
+
+577
+00:42:22,860 --> 00:42:25,200
+أحنا جايين على المحاضرة كانت بالظبط كانت تشت
+
+578
+00:42:25,200 --> 00:42:29,720
+الدنيا و شمينا ريحة الشتاء هو مش راحة الشتاء ريحة
+
+579
+00:42:29,720 --> 00:42:32,640
+الأكتينوميسيتس و الأكتينوميسيتس من الميكروبات
+
+580
+00:42:32,640 --> 00:42:37,380
+العظيمة اللي قلنا عنها كمصدر ال antibiotics في
+
+581
+00:42:37,380 --> 00:42:42,320
+multiocular sporangia برضه كلمة Sporangia هي مصطرح
+
+582
+00:42:42,320 --> 00:42:46,520
+من الفطريات جاي و كلمة أكتينوميسيتس مش بعد أكتينو
+
+583
+00:42:49,460 --> 00:42:54,780
+أكتين .. خيوط .. أكتين و معناها خيوط أصلا ف ..
+
+584
+00:42:54,780 --> 00:42:58,780
+يعني .. يعني النمو تبعها خيطي وبتالي تشبه إلى حد
+
+585
+00:42:58,780 --> 00:43:02,560
+كبير الفطريات وكان في .. في .. في .. في بداية
+
+586
+00:43:02,560 --> 00:43:05,840
+تصنيفها .. كان في بداية تصنيفها كان تصنف بعض
+
+587
+00:43:05,840 --> 00:43:10,040
+الأحيان مع الفطريات لكن لما اكتشفوا أنه إلها صفات
+
+588
+00:43:10,040 --> 00:43:14,670
+ال .. ال .. ال brokaryotes صنفوها مع الكلمة
+
+589
+00:43:14,670 --> 00:43:18,530
+multiocular sporangia طال علي هنا هي خيارت من هنا
+
+590
+00:43:18,530 --> 00:43:20,990
+فاكرين ال .. ال floating body اللي حاكينا عنه قبل
+
+591
+00:43:20,990 --> 00:43:25,630
+شهر؟ ال sporangia هي organs غالبا بتكون
+
+592
+00:43:25,630 --> 00:43:30,330
+microscopic للفطرية تشبه ال floating body و بتحمل
+
+593
+00:43:30,330 --> 00:43:33,110
+على .. بتحمل فيه ال spores ال spores اللي هي
+
+594
+00:43:33,110 --> 00:43:38,410
+الأبواغ اللي .. اللي .. اللي بتستخدم لمين؟ للتكاثر
+
+595
+00:43:38,410 --> 00:43:39,610
+مش لل ..
+
+596
+00:43:45,380 --> 00:43:49,560
+multi-echelon يعني خلّينا نقول إن في إلها أكتر من
+
+597
+00:43:49,560 --> 00:43:58,060
+من مكان على الخط الواحد أكتينو planets طبعا برضه
+
+598
+00:43:58,060 --> 00:44:01,920
+قريبة من ال أكتينو ميسيت في ال strip to mice و ال
+
+599
+00:44:01,920 --> 00:44:04,700
+strip to mice كانت تصمم في يوم من أيام مع ال
+
+600
+00:44:04,700 --> 00:44:09,520
+أكتينو mice و ال strip to mice إذا تتأكدوا من
+
+601
+00:44:09,520 --> 00:44:13,990
+المعلومة اتذكروا ال strip to miceالـ Streptomycin
+
+602
+00:44:13,990 --> 00:44:18,730
+هو Antibiotic يضاهي في شهرته البنسالين لأنه قديم
+
+603
+00:44:18,730 --> 00:44:22,430
+طبعا التلان أصبحوا بلا شهرة الآن لأن البكتيريا
+
+604
+00:44:22,430 --> 00:44:26,970
+صارت مقاومة لكثير منهم أو كثير من البكتيريا صارت
+
+605
+00:44:26,970 --> 00:44:31,350
+مقاومة لهم لكن هو من أوائل ال .. ال .. ال .. ال ..
+
+606
+00:44:31,350 --> 00:44:32,190
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+607
+00:44:32,190 --> 00:44:33,250
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+608
+00:44:33,250 --> 00:44:33,790
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+609
+00:44:33,790 --> 00:44:34,230
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+610
+00:44:34,230 --> 00:44:34,250
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+611
+00:44:34,250 --> 00:44:34,290
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+612
+00:44:34,290 --> 00:44:34,770
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+613
+00:44:34,770 --> 00:44:39,030
+..
+
+614
+00:44:40,070 --> 00:44:44,510
+في النمدورو مايسيتس برضه مجموعة من الميكروبات في
+
+615
+00:44:44,510 --> 00:44:48,410
+ثيرمو مونوسبورا and redgenera ثيرمو واضح من اسمها
+
+616
+00:44:48,410 --> 00:44:54,210
+انها تحمل الحرارة وكمان في ثيرمو اكتينو مايسيتس
+
+617
+00:44:54,210 --> 00:44:59,370
+برضه اكتينو مايسيتس مجموعة قدرة
+
+618
+00:44:59,370 --> 00:45:05,250
+خاصة على تحمل الحرارة مجموعة تلات تلاتيناللي
+
+619
+00:45:05,250 --> 00:45:10,070
+ماقدرناش نصنفه في أي من ال 32 حطنا خيار المجموعة
+
+620
+00:45:10,070 --> 00:45:14,490
+لحاله لحين لما لاقيه القضوة طب عارفين دي مجموعة من
+
+621
+00:45:14,490 --> 00:45:18,690
+ال .. من الميكروبات؟ لازم أطرقلكم إياهم طيب ع
+
+622
+00:45:18,690 --> 00:45:20,690
+السنية Actinomycetes
+
+623
+00:45:23,940 --> 00:45:27,040
+خدوا بالكم هذا عبارة عن خلطة من الميكروبات مش نوع
+
+624
+00:45:27,040 --> 00:45:31,180
+واحد وإنما to make the names of microorganism
+
+625
+00:45:31,180 --> 00:45:37,600
+familiar to you كمرة مش للحفظ وإنما لجالها يعني
+
+626
+00:45:37,600 --> 00:45:44,420
+ملوفة لديكم باسيلاس، بوداتيلا، بوريليا، بروسيلا،
+
+627
+00:45:44,420 --> 00:45:50,640
+كاليسي فايروس، كامبيلو بارتر، كانديدا، كلاميديا،
+
+628
+00:45:50,640 --> 00:45:51,360
+كلستريديوم
+
+629
+00:45:54,670 --> 00:45:59,510
+Cryptococcus, Enterovirus, Escherichia طبعا هذه
+
+630
+00:45:59,510 --> 00:46:04,590
+المشهورة ساعدة انكوا Flavivirus مافيش داعي انه كل
+
+631
+00:46:04,590 --> 00:46:10,650
+مرة نقول Flavivirus هو فيروس من اما بينتهي ب ..
+
+632
+00:46:10,650 --> 00:46:13,650
+Hemophilus مكتوبة كانت انكوا خاطئة بالمحاضرة اللي
+
+633
+00:46:13,650 --> 00:46:17,110
+نزلتها عدلناها Hemophilus Hepatitis viruses
+
+634
+00:46:17,110 --> 00:46:19,290
+Helicobacter مش Helicobacter
+
+635
+00:46:22,650 --> 00:46:26,030
+Helicobacter أي واحد عنده قرحة معينية يعرفه أنه هي
+
+636
+00:46:26,030 --> 00:46:31,150
+المسبب لهذه القرحة Helicobacter Hemorrhagic fever
+
+637
+00:46:31,150 --> 00:46:35,970
+virus Herpes viruses Legionella Leptospira
+
+638
+00:46:35,970 --> 00:46:41,190
+Mycobacterium Mycoplasma أسماء صارت في جزء منها
+
+639
+00:46:41,190 --> 00:46:44,750
+familiar بالفعل Neisseria Nocardia
+
+640
+00:46:44,750 --> 00:46:48,770
+Orthomyoxiviruses اللي بتشمل ال influenza virus
+
+641
+00:46:49,870 --> 00:46:54,670
+Papillomavirus Paramyoxivirus لاحظوا الأسماء اللي
+
+642
+00:46:54,670 --> 00:46:58,750
+طويلة مامنعاش يعني قدرة على لفظها مرة واحدة
+
+643
+00:46:58,750 --> 00:47:03,530
+بنجبطها Paramyoxivirus Orthomyoxiviruses
+
+644
+00:47:03,530 --> 00:47:07,050
+Parvoviruses
+
+645
+00:47:07,050 --> 00:47:10,010
+Prions ال Prions هحطوا تحتها خط ونكلم عنها بعدين
+
+646
+00:47:10,010 --> 00:47:14,950
+Pseudomonas Rabies virus Rubella virus
+
+647
+00:47:14,950 --> 00:47:16,470
+Retroviruses
+
+648
+00:47:18,290 --> 00:47:21,250
+Rhinoviruses ريكيزيا مرض معانا ريكيزيا اللي سيجيب
+
+649
+00:47:21,250 --> 00:47:25,890
+البقايا Rotavirus Salmonella Shigella
+
+650
+00:47:25,890 --> 00:47:30,170
+Staphylococcus أو Staphylococci Stryptococci
+
+651
+00:47:30,170 --> 00:47:33,030
+Triponema مرض معانا ال spiral اللي رسلناها على
+
+652
+00:47:33,030 --> 00:47:38,110
+الصطبورة هذه Triponema palidumاليوريا بلازما و ال
+
+653
+00:47:38,110 --> 00:47:41,530
+vibrio و الرسمية طبعا انا بقولكوا يعني بالبداية
+
+654
+00:47:41,530 --> 00:47:45,730
+يمكن يكون في صعوبة في نطق الكلمات لكن بعد مرة او
+
+655
+00:47:45,730 --> 00:47:49,250
+مرتين بتصير يعني محببة و مقفلة
+
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/ipbsvDeQAIA_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/ipbsvDeQAIA_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..43f6d580f0618c023649a0958fe51819e938126c
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/ipbsvDeQAIA_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 2715, "start": 20.67, "end": 27.15, "text": "بسم الله الرحمن الرحيم نكمل ان شاء الله من حياتهم الدائمة bacterial infection and immunity", "tokens": [3555, 38251, 21984, 34892, 5016, 27842, 34892, 5016, 32640, 8717, 24793, 1211, 16472, 13412, 16606, 21984, 9154, 11331, 1829, 9307, 16095, 32748, 16373, 46007, 35632, 11764, 293, 22701], "avg_logprob": -0.2342403037794705, "compression_ratio": 1.2454545454545454, "no_speech_prob": 0.0, "words": [{"start": 20.67, "end": 20.87, "word": "بسم", "probability": 0.915771484375}, {"start": 20.87, "end": 21.03, "word": " الله", "probability": 0.96826171875}, {"start": 21.03, "end": 21.31, "word": " الرحمن", "probability": 0.9627278645833334}, {"start": 21.31, "end": 21.77, "word": " الرحيم", "probability": 0.9925130208333334}, {"start": 21.77, "end": 22.63, "word": " نكمل", "probability": 0.9064127604166666}, {"start": 22.63, "end": 22.69, "word": " ان", "probability": 0.62548828125}, {"start": 22.69, "end": 22.89, 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{"start": 1504.15, "end": 1504.77, "word": " العادمة", "probability": 0.9539388020833334}, {"start": 1504.77, "end": 1505.43, "word": " تبعتها", "probability": 0.91650390625}, {"start": 1505.43, "end": 1506.47, "word": " داخليا", "probability": 0.8812255859375}, {"start": 1506.47, "end": 1506.61, "word": " في", "probability": 0.93505859375}, {"start": 1506.61, "end": 1507.31, "word": " المستشفيات", "probability": 0.9814453125}, {"start": 1507.31, "end": 1507.89, "word": " قبل", "probability": 0.9833984375}, {"start": 1507.89, "end": 1508.27, "word": " ضقة", "probability": 0.6962890625}, {"start": 1508.27, "end": 1508.63, "word": " إلى", "probability": 0.68798828125}, {"start": 1508.63, "end": 1509.65, "word": " المجتمع", "probability": 0.9935302734375}], "temperature": 1.0}, {"id": 65, "seek": 151640, "start": 1510.3, "end": 1516.4, "text": "يبقى تصنيف هذا question مش كويس الميزة مع بعض ونكمل ان شاء الله المرة القادمة بإذن الله نفس الامور", "tokens": [1829, 3555, 4587, 7578, 6055, 9381, 22653, 5172, 23758, 1168, 37893, 9122, 45865, 3794, 9673, 1829, 11622, 3660, 20449, 45030, 11242, 4032, 1863, 24793, 1211, 16472, 13412, 16606, 21984, 9673, 25720, 25062, 18513, 46007, 4724, 28814, 8848, 1863, 21984, 8717, 36178, 2423, 10943, 13063], "avg_logprob": -0.2961805608537462, "compression_ratio": 1.328125, "no_speech_prob": 0.0, "words": [{"start": 1510.3, "end": 1510.78, "word": "يبقى", "probability": 0.7894287109375}, {"start": 1510.78, "end": 1511.26, "word": " تصنيف", "probability": 0.876708984375}, {"start": 1511.26, "end": 1511.4, "word": " هذا", "probability": 0.701171875}, {"start": 1511.4, "end": 1511.66, "word": " question", "probability": 0.493896484375}, {"start": 1511.66, "end": 1511.76, "word": " مش", "probability": 0.85107421875}, {"start": 1511.76, "end": 1512.24, "word": " كويس", "probability": 0.9720052083333334}, {"start": 1512.24, "end": 1513.7, "word": " الميزة", "probability": 0.673004150390625}, {"start": 1513.7, "end": 1513.74, "word": " مع", "probability": 0.86328125}, {"start": 1513.74, "end": 1514.24, "word": " بعض", "probability": 0.900146484375}, {"start": 1514.24, "end": 1514.84, "word": " ونكمل", "probability": 0.81298828125}, {"start": 1514.84, "end": 1514.86, "word": " ان", "probability": 0.66748046875}, {"start": 1514.86, "end": 1515.02, "word": " شاء", "probability": 0.958984375}, {"start": 1515.02, "end": 1515.08, "word": " الله", "probability": 0.9541015625}, {"start": 1515.08, "end": 1515.28, "word": " المرة", "probability": 0.891845703125}, {"start": 1515.28, "end": 1515.66, "word": " القادمة", "probability": 0.8889973958333334}, {"start": 1515.66, "end": 1515.86, "word": " بإذن", "probability": 0.62860107421875}, {"start": 1515.86, "end": 1515.92, "word": " الله", "probability": 0.9326171875}, {"start": 1515.92, "end": 1516.16, "word": " نفس", "probability": 0.761962890625}, {"start": 1516.16, "end": 1516.4, "word": " الامور", "probability": 0.4977213541666667}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 1517.377625, "duration_after_vad": 1429.089999999998}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/ipbsvDeQAIA_raw.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/ipbsvDeQAIA_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..5fd11e81937a87126cb95dba2c2ce0e86cd5b509
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/ipbsvDeQAIA_raw.srt
@@ -0,0 +1,1332 @@
+1
+00:00:20,670 --> 00:00:23,410
+بسم الله الرحمن الرحيم نكمل ان شاء الله من حياتهم
+
+2
+00:00:23,410 --> 00:00:29,310
+الدائمة bacterial infection and immunityاللوحة
+
+3
+00:00:29,310 --> 00:00:32,030
+اللي أمامكوا هي العنوان .. لوحة العنوان فيها
+
+4
+00:00:32,030 --> 00:00:35,910
+جالوتين مهمين جدا اللوحة الو .. او الصورة اللي ..
+
+5
+00:00:35,910 --> 00:00:39,350
+اللي بتغير ألوانها في المنطقة هذه حاجة بيسموها
+
+6
+00:00:39,350 --> 00:00:41,810
+chain of transmission ايش هي ال chain of
+
+7
+00:00:41,810 --> 00:00:45,450
+transmission؟ سلسلة الانتقال، لو بينتقل الميكروب
+
+8
+00:00:45,450 --> 00:00:51,390
+من إنسان إلى إنسان، من حيوان إلى إنسان، تمام؟ في
+
+9
+00:00:51,390 --> 00:00:55,930
+سلسلة من .. او خطوات معينة لازم تحدث، يجب أن تحدث
+
+10
+00:00:57,020 --> 00:01:00,680
+هذه الخطوات بنسميها chain of transmission فالصورة
+
+11
+00:01:00,680 --> 00:01:03,740
+اللي قدامكم ممكن تلاقوها بشكل سلسل في ال internet
+
+12
+00:01:03,740 --> 00:01:06,960
+أو في أماكن أخرى لكن في الآخر بتعبر عن نفس المعنى
+
+13
+00:01:06,960 --> 00:01:10,880
+بتعبر عن نفس المعنى ليش سميناها سلسلة لإن لو
+
+14
+00:01:10,880 --> 00:01:14,540
+بيصينا السلسلة في مكان احنا بنقطع ال chain of
+
+15
+00:01:14,540 --> 00:01:18,950
+transmissionوبالتالي احنا من وجبنا لما نعمل
+
+16
+00:01:18,950 --> 00:01:22,810
+control لل micro organism او لل infection انه نفهم
+
+17
+00:01:22,810 --> 00:01:27,650
+ال infection cycle او ال chain of transmission فهم
+
+18
+00:01:27,650 --> 00:01:33,150
+معمق بحيث انه نفكر كيف ممكن نقطع هذا ال cycle في
+
+19
+00:01:33,150 --> 00:01:35,830
+اي جانب انه الأسهل انه الأرخص انه الأضمن انه
+
+20
+00:01:35,830 --> 00:01:39,650
+الأسرع كل هذه النقاط ممكن نفهمها ان شاء الله من
+
+21
+00:01:39,650 --> 00:01:44,570
+هذه المحاضرة الصورة التانية معبرة عن عين و فيروسات
+
+22
+00:01:45,900 --> 00:01:49,500
+الفيروسات اللي من ضمنها ال influenza اللي من ضمنها
+
+23
+00:01:49,500 --> 00:01:52,580
+الرشح انتوا كلكوا متخيلين انها بتنتقل اقابل مين ال
+
+24
+00:01:52,580 --> 00:01:58,080
+respiratory tract فقط، لكن ممكن finger to eye، ايش
+
+25
+00:01:58,080 --> 00:02:03,570
+يعني finger to eye؟ يعني واحد .. راكز ..فأسطح واحد
+
+26
+00:02:03,570 --> 00:02:06,950
+جنبه كان عاطس وحاطط الفيروس على الطاولة واحنا
+
+27
+00:02:06,950 --> 00:02:10,330
+لمسناه و بعد شويه أجى عنده شعور بالحكا ودخل إصبعه
+
+28
+00:02:10,330 --> 00:02:15,970
+في عينه نقل الفيروس من ال surface إلى ال .. ال ..
+
+29
+00:02:15,970 --> 00:02:18,450
+ال .. ال nucleus membrane في العينه وبالتالي يمكن
+
+30
+00:02:18,450 --> 00:02:22,370
+يعمل infection إذا هي source مهم لمن؟ لل infection
+
+31
+00:02:24,680 --> 00:02:29,240
+في العلاقات ما بين اكتر من مخلوقين او مخلوقين او
+
+32
+00:02:29,240 --> 00:02:33,040
+اكتر قد تنقسم العلاقات الى ثلاث أشكال اما
+
+33
+00:02:33,040 --> 00:02:36,560
+commensalism او parasitism او mutualism كلكوا
+
+34
+00:02:36,560 --> 00:02:40,660
+درستهم في أحياء ألف و درستهم في ثانوية و قبل ذلك
+
+35
+00:02:40,660 --> 00:02:46,280
+لكن اللى انا بركز عليها في ال medical microbiology
+
+36
+00:02:46,280 --> 00:02:46,860
+علاقة
+
+37
+00:02:49,640 --> 00:02:53,500
+ال Parasitism ليش؟ لأنه فيه effect، harmful
+
+38
+00:02:53,500 --> 00:02:57,080
+effect، واحد بيستفيد اللي هو ال pathogen ال
+
+39
+00:02:57,080 --> 00:03:00,360
+parasite و واحد بينضر اللي هو ال host اللي هو
+
+40
+00:03:00,360 --> 00:03:02,960
+الإنسان في الحالة و ابتدا ال medical microbiome قد
+
+41
+00:03:02,960 --> 00:03:08,500
+أضطرق بشيء من الذكر ل ال commensals اللي هي
+
+42
+00:03:08,500 --> 00:03:14,470
+بتستفيد هي و الجسم بندرش تمام؟و قد نتطرق إلى ال
+
+43
+00:03:14,470 --> 00:03:17,650
+mutualism زي ال normal flora احنا بنوفرلها مكان و
+
+44
+00:03:17,650 --> 00:03:21,550
+هي بتعطيلنا vitamin K بنوفرلها مكان و هي بتساعدنا
+
+45
+00:03:21,550 --> 00:03:25,570
+في الحماية من infection من others لكن في الغالب
+
+46
+00:03:25,570 --> 00:03:28,930
+تركيزنا هيكون على أي واحدة منهم على اللي هي ال
+
+47
+00:03:28,930 --> 00:03:35,010
+palestine طيب مدام سبب نتكلمين على ال normal flora
+
+48
+00:03:35,010 --> 00:03:38,210
+كلمة flora بنات معناها باللغة العربية نباتات فلما
+
+49
+00:03:38,210 --> 00:03:41,510
+بيقول flora أو فلسطين و بمكاننا بيقول نباتات
+
+50
+00:03:41,510 --> 00:03:42,170
+فلسطين
+
+51
+00:03:47,960 --> 00:03:54,600
+الإنسان بشكل دائم هو الاسم القديم normal flora
+
+52
+00:03:54,600 --> 00:04:00,880
+طبعا لأنه زمان كانت تدرس البكتيريا تحت علم البطن
+
+53
+00:04:00,880 --> 00:04:07,200
+يعني علم النبات لأنهم كانوا صنفوها أيضا نباتفاطلق
+
+54
+00:04:07,200 --> 00:04:10,120
+اي لقب flora لكن الان و بعد ما احنا خلاص يعني صارت
+
+55
+00:04:10,120 --> 00:04:14,740
+الى مملكة لحاله تمام صار من الجدير انه او من
+
+56
+00:04:14,740 --> 00:04:17,360
+الأشدر ان احنا نسميها اسم خاص فيها فسموها
+
+57
+00:04:17,360 --> 00:04:21,900
+microbiota فسماء سواء اسمته normal flora او normal
+
+58
+00:04:21,900 --> 00:04:26,560
+microbiota المعنى هو واحد من فوائد جبل من الفوائد
+
+59
+00:04:26,560 --> 00:04:29,160
+انا قولت ان الجسم بنقسم الى قسمين مرة ده بتذكروا
+
+60
+00:04:29,160 --> 00:04:32,680
+بنقسم الى sterile و non sterile ال non sterile
+
+61
+00:04:32,680 --> 00:04:37,080
+بالتأكيد بحتوى على normal floraو ال normal flora
+
+62
+00:04:37,080 --> 00:04:41,040
+إلها فوائد عظيمة، يعني مش موجودة هيك بس عبثا، لأ
+
+63
+00:04:41,040 --> 00:04:45,860
+إلها فوائد عظيمة و كثيرة، من أهم فوائدها، بديش
+
+64
+00:04:45,860 --> 00:04:48,980
+أقول رقم واحد، رقم اتنين، رقم اتنين هي من أهم
+
+65
+00:04:48,980 --> 00:04:52,780
+فوائدها، حاجة بيسميها colonization resistance،
+
+66
+00:04:52,780 --> 00:04:56,740
+بالعربي مقاومة الاستعمار، يعني مش لحاجة أهل فلسطين
+
+67
+00:04:56,740 --> 00:04:59,700
+بتقاوم الاستعمار، كمان فينا اللي هو ال normal
+
+68
+00:04:59,700 --> 00:05:03,160
+microbiota، ما معناه مقاومة الاستعمار بهذا أوجه
+
+69
+00:05:03,160 --> 00:05:10,570
+المفهوم تبقىتخيلوا أنكم كلكم من الأصدقاء، تمام؟ و
+
+70
+00:05:10,570 --> 00:05:14,870
+أنكم أبين الكراسي اللي في القاعة دي كلها، لو إيجي
+
+71
+00:05:14,870 --> 00:05:17,630
+واحد من الأعداء بدي يحطله موطق قدم، بيلاجي مكان
+
+72
+00:05:17,630 --> 00:05:21,950
+يقعد فيه، يبقى وجودكم في هذا المكان فقط وجودكم،
+
+73
+00:05:21,950 --> 00:05:27,050
+تمام؟محتلين مواقع معينة في هذا المكان مانع آخرين
+
+74
+00:05:27,050 --> 00:05:32,330
+من انهم يستوطنوا و يستعمروا كولونايزايشن و أول
+
+75
+00:05:32,330 --> 00:05:36,330
+خطوة في ال infection كولونايزايشن و أول خطوة في
+
+76
+00:05:36,330 --> 00:05:40,630
+الكولونايزايشن attachment مانجلاش مكان نقعد فيه
+
+77
+00:05:40,630 --> 00:05:46,650
+تمام ال pathogen مع السلام مش بس هيك لو كان فينا
+
+78
+00:05:46,650 --> 00:05:51,650
+pathogen كمان و جايب معاه عداد كبيرة يعني ممكن
+
+79
+00:05:51,650 --> 00:05:57,320
+ينافسفاكرين لما درسنا منتجات البكتيريا اللي من
+
+80
+00:05:57,320 --> 00:06:00,520
+ضمنها ال toxins درسنا منتج اسمه bactericin رقم
+
+81
+00:06:00,520 --> 00:06:08,680
+أربع رقم تلاتة bactericin اللي هي antibiotic
+
+82
+00:06:08,680 --> 00:06:12,660
+-like سميناها antibiotic-like compounds produced
+
+83
+00:06:12,660 --> 00:06:12,940
+by
+
+84
+00:06:16,130 --> 00:06:21,010
+كده من المنافسة، كمان هذا بيساعد على أنه نمنع ال
+
+85
+00:06:21,010 --> 00:06:24,130
+colonization، إذا في عندي رقم اتنين و تلاتة
+
+86
+00:06:24,130 --> 00:06:29,890
+بيساهموا في منع الأمراض، في منع حدوث الأمراض، ليش؟
+
+87
+00:06:29,890 --> 00:06:34,190
+بيمنعوا استيطان أو استعمار اللي هم ال pathogenic
+
+88
+00:06:34,190 --> 00:06:38,290
+microorganismرقم واحد بعض البكتيريا بتنتج vitamin
+
+89
+00:06:38,290 --> 00:06:42,970
+K و vitamin K جيد للانسان بيستفيد منه رقم اربع
+
+90
+00:06:42,970 --> 00:06:47,370
+بقول ان ال normal flora قد تساهم في ال stimulation
+
+91
+00:06:47,370 --> 00:06:52,390
+ل special organs او special tissue في مجموعة من ال
+
+92
+00:06:52,390 --> 00:06:57,670
+lymph nodes موجودة في الأمعاء اسمها biopatches و
+
+93
+00:06:57,670 --> 00:07:02,650
+هي specialized lymph node موجودين في الأمعاء ايش
+
+94
+00:07:02,650 --> 00:07:08,870
+ال lymph nodes؟بيوصفوها في علم المناعة انها the
+
+95
+00:07:08,870 --> 00:07:13,030
+budget check points نقاط التفتيش تبع الجسم ايش هي
+
+96
+00:07:13,030 --> 00:07:18,390
+نقاط التفتيش؟ تخيلوا ان القاع هذه مخصصة لطالبات
+
+97
+00:07:18,390 --> 00:07:21,610
+قسم العلم الطبي المخبرية سنة تانية ممنوع يدخلها
+
+98
+00:07:21,610 --> 00:07:24,990
+سنة تالتة ممنوع يدخلها سنة أولى فلازم يكون على
+
+99
+00:07:24,990 --> 00:07:30,730
+الباب حد يعمل check صح؟ على البطاقة انه فقط طالبات
+
+100
+00:07:30,730 --> 00:07:34,190
+سنة تانية مسمحوا لهم فال .. ال .. ال .. ال .. ال
+
+101
+00:07:34,190 --> 00:07:37,180
+..الـ Lymph node اللي مروضة في ال bio patches
+
+102
+00:07:37,180 --> 00:07:41,800
+بتكون بدورة عظيم جدا في مناء العدوى و خاصة انا
+
+103
+00:07:41,800 --> 00:07:44,920
+هنتكلم عن ال intestinal tract مليارات و ملايين من
+
+104
+00:07:44,920 --> 00:07:49,640
+الانواع من البكتيريا وبالتالي هي نوع الميكروبيوتر
+
+105
+00:07:49,640 --> 00:07:53,880
+عملت stimulation لهذا النسيج يعني خلته دايما اه
+
+106
+00:07:53,880 --> 00:07:56,800
+متحفز وساعد في ال development تبعه
+
+107
+00:08:04,260 --> 00:08:07,620
+colonization resistance ذكرتها في الأمعاب لكن
+
+108
+00:08:07,620 --> 00:08:11,700
+تذكروا الأنثى كمان إيها خاصية الفمية الفاجاينة
+
+109
+00:08:11,700 --> 00:08:14,820
+فيها normal microbiota و بتعمل colonization
+
+110
+00:08:14,820 --> 00:08:19,300
+resistance الستات الحوامل بيصير عندهم urinary
+
+111
+00:08:19,300 --> 00:08:22,000
+tract infection أو vagina infection أكتر من اللي
+
+112
+00:08:22,000 --> 00:08:25,560
+مش حوامل عارفين ليش؟ لأن ال hormonal changes اللي
+
+113
+00:08:25,560 --> 00:08:29,920
+بتصير عندها بتأثر على ال normal flora وبالتالي
+
+114
+00:08:29,920 --> 00:08:33,950
+بيصير ال normal flora مش طبيعيةبصير الابتزان تبعها
+
+115
+00:08:33,950 --> 00:08:38,210
+في خلال فبصير فينا إمكانية colonization ل other
+
+116
+00:08:38,210 --> 00:08:43,690
+microorganisms هحاكيلكوا كمان نقطة كتير مهمة
+
+117
+00:08:43,690 --> 00:08:51,970
+concept بدنا نفهمها كويس لو إحنا اتنين شخصين بدنا
+
+118
+00:08:51,970 --> 00:08:56,430
+نروح على الغرف التانية و بدنا نعمله مشكلة مشكلة
+
+119
+00:08:56,430 --> 00:08:59,760
+لكوا كديش البنات اللي قدامهم خمسين بناتراحوا
+
+120
+00:08:59,760 --> 00:09:02,660
+البنتين هؤلاء المسكين كل واحدة معاها جلم ولا سكينة
+
+121
+00:09:02,660 --> 00:09:06,680
+ولا حاجة ورفعت بإن أنا أقتلكوا 150 إبنة، ليش
+
+122
+00:09:06,680 --> 00:09:09,900
+بيكونوا عاملهم واقع لعمد؟ لأ لأ مش بصيروا يضحكوا،
+
+123
+00:09:09,900 --> 00:09:15,520
+جانوا على أنفسهم، هم أعلنوا عن عدوتهم تمام؟ وهم لا
+
+124
+00:09:15,520 --> 00:09:18,580
+يمتلكوا من القوة شيء، تعرفوا إن البكتيريا أذكى
+
+125
+00:09:18,580 --> 00:09:23,480
+منكم، من البنتين جزء اللي راحوا يعنيالبكتيريا لن
+
+126
+00:09:23,480 --> 00:09:26,640
+تقلل عن نفسها .. و أقولكوا ال analogy تبعها
+
+127
+00:09:26,640 --> 00:09:30,180
+للبكتيريا، لو خليتين بكتيريتين قرروا أنهم .. دخلوا
+
+128
+00:09:30,180 --> 00:09:32,820
+الدسيل، و قرروا أنهم ينطلوا التوكسين، يعني رفعوا
+
+129
+00:09:32,820 --> 00:09:38,720
+السلاح تبعهم، التوكسين لن يكون له أي تأثير، لأن
+
+130
+00:09:38,720 --> 00:09:43,240
+كميته إيش هتكون؟ من two cells، قليل جدا، لكن هيكون
+
+131
+00:09:43,240 --> 00:09:46,020
+له أثر أنهم رفعوا السكين، بمعنى آخر جاز المناعة
+
+132
+00:09:46,020 --> 00:09:52,440
+تنبه لهم، فقتلوايبجي أعلنهم عن نفسهم مبكرا كان سيئ
+
+133
+00:09:52,440 --> 00:09:57,060
+سبب في موتهم فالبكتيريا ذكية البكتيريا لن تعلن عن
+
+134
+00:09:57,060 --> 00:10:01,400
+نفسها إلا إذا وصل عددها يبجي معنا الحكي كلمة وصل
+
+135
+00:10:01,400 --> 00:10:06,860
+عددها يبجي معنا معاها تقنية عندها تقنية تعرف فيها
+
+136
+00:10:06,860 --> 00:10:09,560
+من خلالها الأدب تبعها انتوا ماعندكوا التقنية هذه
+
+137
+00:10:09,560 --> 00:10:12,940
+يعني لو غمنا واحدة منكم احنا وقلنا للطالبات ادخلوا
+
+138
+00:10:12,940 --> 00:10:16,220
+الصف يلا بنت انت اللي مغمى أكمل واحدة في الصف مش
+
+139
+00:10:16,220 --> 00:10:20,040
+هتعرفوا بس البكتيريا بتعرفواالظهرة بنسميها Quorum
+
+140
+00:10:20,040 --> 00:10:25,640
+Sensing Quorum Sensing إحساس بالعدد الكلي Quorum
+
+141
+00:10:25,640 --> 00:10:30,440
+Sensing طبعا حاجة وقت نشرحها بتفاصيل أكتر كيف بتتم
+
+142
+00:10:30,440 --> 00:10:33,860
+لكن بنلفها مدى النقطة إيش .. إيش الدلالة اللي أنا
+
+143
+00:10:33,860 --> 00:10:38,440
+بدي أوصلها في هذا الموضوع ال normal microbiota
+
+144
+00:10:38,440 --> 00:10:44,660
+موجودة بأنواع مختلفة في مكان واحد أنواع مختلفة
+
+145
+00:10:44,660 --> 00:10:50,440
+بعداد محددة بعداد محددةهذه الأعداد المحددة غير
+
+146
+00:10:50,440 --> 00:10:56,360
+كافية للإعلان عن نفسها لإنتاج virulence factors
+
+147
+00:10:56,360 --> 00:11:03,200
+عوامل الشراسة اللي من ضمنها ال toxins نفترض الشجهة
+
+148
+00:11:03,200 --> 00:11:09,680
+هذه هم المجموعة اللي عاجلة و ممتازة وجاينة دور
+
+149
+00:11:09,680 --> 00:11:15,770
+كويس لإن عددهم قليلجبنا دموع و رشينا عليكم انتوا
+
+150
+00:11:15,770 --> 00:11:18,350
+المجموعة الكبرى اللى كانت مسيطرة على المجموعه دموع
+
+151
+00:11:18,350 --> 00:11:23,820
+مانعاها انها تكبر و موتناالان الغذاء اللى كان مخصص
+
+152
+00:11:23,820 --> 00:11:27,820
+لكوا المساحة صارت فاضية مين هاستغلها؟ هاستغل عددهم
+
+153
+00:11:27,820 --> 00:11:31,920
+ضعف او اضعاف عندهم الأول اذا وصل العدد ل column
+
+154
+00:11:31,920 --> 00:11:35,060
+sensing بيبطلوا عاجلين وكويسين ايش بيصيروا الان
+
+155
+00:11:35,060 --> 00:11:40,100
+ينتجوا ال virus factors اللى من ضمنها ال toxin
+
+156
+00:11:40,100 --> 00:11:45,240
+لذلك هناك أمراض قد تنشأ من وجود خلل في ال normal
+
+157
+00:11:45,240 --> 00:11:49,860
+microbiota وصلت صورة بعدين هم normal لما بيكونوا
+
+158
+00:11:49,860 --> 00:11:53,700
+في مكانهم normalلما بطلع من مكانهم إلى مكان آخر
+
+159
+00:11:53,700 --> 00:11:58,860
+ببطله normal يعني أعطيكم مثال واضح وصري الشريشيا
+
+160
+00:11:58,860 --> 00:12:02,820
+كولاي is a normal flora of the intestinal tract
+
+161
+00:12:02,820 --> 00:12:06,640
+الشريشيا
+
+162
+00:12:06,640 --> 00:12:09,200
+كولاي is number one cause of urinary tract
+
+163
+00:12:09,200 --> 00:12:14,320
+infection يبقى لما انتقلت من ال intestine إلى ال
+
+164
+00:12:14,320 --> 00:12:18,360
+urinary tract عملت infectionيبقى مش كلهم normal
+
+165
+00:12:18,360 --> 00:12:20,900
+flora يعني normal flora يعني مصطلح تطلق عليهم
+
+166
+00:12:20,900 --> 00:12:24,220
+normal flora لأ في مكانه هو normal flora و ربما
+
+167
+00:12:24,220 --> 00:12:30,060
+يكون العدد كمان عامل محدد مهم و بما أن احنا ذكرنا
+
+168
+00:12:30,060 --> 00:12:34,260
+كلمة عدد تعالوا نتكلم على ايش الأوامن التلاتة أصلا
+
+169
+00:12:34,260 --> 00:12:40,340
+اللي بتساهم في ال infection ال host immunity ال
+
+170
+00:12:40,340 --> 00:12:48,750
+type ofmicroorganism وكلمة type يحق لشراسته و ال
+
+171
+00:12:48,750 --> 00:12:52,170
+number اللي دخل ال number تبع ال microorganism هاي
+
+172
+00:12:52,170 --> 00:12:57,970
+تلت عوامل كمان مرة ال host immunityالـType وكنا
+
+173
+00:12:57,970 --> 00:13:00,690
+كامرة الـType و الـOrganism كنا إشارة لمن؟
+
+174
+00:13:00,690 --> 00:13:06,250
+للـVirals رقم تلاتة الـNumber في ضعفات أخرى لكن هي
+
+175
+00:13:06,250 --> 00:13:10,750
+أهم التلت عوامل اللي بتساهم أنه يصير Infection و
+
+176
+00:13:10,750 --> 00:13:12,690
+لا ما يصير إيش Infection و أعطيكم إتالة بسيطة عشان
+
+177
+00:13:12,690 --> 00:13:18,930
+نسينا أن الشجلوسز شجلة تمام حتى عند الإنسان الصحيح
+
+178
+00:13:18,930 --> 00:13:22,290
+الإنسان اللي عنده جهاز ملاعق كويس ال number
+
+179
+00:13:22,290 --> 00:13:27,840
+requiredالشجلة number شوية من one to ten cells
+
+180
+00:13:27,840 --> 00:13:34,000
+خلية لعشر خلايا في المقابل لو ال type اختلف من
+
+181
+00:13:34,000 --> 00:13:38,120
+shigella ل vibrio محتاج لمليون محتاج لمليون ايش
+
+182
+00:13:38,120 --> 00:13:43,540
+العامل اللي اختلف نوع البكتيريا فادة الاختلاف اللي
+
+183
+00:13:43,540 --> 00:13:49,200
+هو ال number يبقى كل type له dosage معينة طب انا
+
+184
+00:13:49,200 --> 00:13:54,190
+هتعطيكم مثال كمان اختر شويةاحنا نقشنا مرة البصيلاس
+
+185
+00:13:54,190 --> 00:13:58,630
+أنتراكسي صح اللي بسبب أنتراكس مينعناج ال sport
+
+186
+00:13:58,630 --> 00:14:05,810
+former هذه ال sports تبعتها إذا بتدخل عبر الجلد
+
+187
+00:14:05,810 --> 00:14:10,670
+بيكفي منها 100 ألف أو أقل من 100 ألف انهم يعملوا
+
+188
+00:14:10,670 --> 00:14:16,330
+infection إذا دخل عبر الجهاز التنفسي بيحتاج إلى
+
+189
+00:14:16,330 --> 00:14:22,430
+مليون sport 100 ألف لمليونرقم مختلف عن بعض كتير
+
+190
+00:14:23,210 --> 00:14:27,290
+عشرة مليون عشان يعمل gastro-intestinal anthrax
+
+191
+00:14:27,290 --> 00:14:32,130
+يبقى ال portal of entry مكان دخول الميكروب كمان له
+
+192
+00:14:32,130 --> 00:14:36,830
+علاقة بمين؟ بالعدد فهي نفس الميكروب تتحدثنا عن نفس
+
+193
+00:14:36,830 --> 00:14:41,790
+الميكروب لما دخل من الجلد الرقم كان أقلهم لما دخل
+
+194
+00:14:41,790 --> 00:14:46,850
+من ال lungs زاد الرقم لما دخل من الفم عبر ال
+
+195
+00:14:46,850 --> 00:14:50,010
+intestine زاد الرقم أكتر وأكتر إذا ال portal of
+
+196
+00:14:50,010 --> 00:14:54,730
+entry كمان عملية مهمةكل اللي ذكرته هنا عشان
+
+197
+00:14:54,730 --> 00:15:02,210
+أقولكوا أنه المصطلحات اللي
+
+198
+00:15:02,210 --> 00:15:07,590
+كلمة pathogen و non pathogen هي مصطلحات للأسف مش
+
+199
+00:15:07,590 --> 00:15:12,920
+absoluteبمعنى أنه أنا نفسي أكون absolute، أقول
+
+200
+00:15:12,920 --> 00:15:17,180
+أبيض يعني non-pathogen، أسود يعني pathogen، لكن
+
+201
+00:15:17,180 --> 00:15:23,160
+للأسف بيدخل إلينا فيروسات وmicrobات إلى أجسامنا،
+
+202
+00:15:23,160 --> 00:15:26,920
+تمام؟ و بأعياد كبيرة، مبيعات كبيرة، و معروفة أنها
+
+203
+00:15:26,920 --> 00:15:29,380
+بتسبب أمراض، لكن ممكن ما تسبب أننا إيش احنا؟
+
+204
+00:15:29,380 --> 00:15:35,150
+أمراض، مريض ال aidsالميكروبات اللي ما بتسببش أمراض
+
+205
+00:15:35,150 --> 00:15:38,110
+إلنا إحنا بتسببله أمراض اللي بجينا نقولها non
+
+206
+00:15:38,110 --> 00:15:45,010
+pathogen عشان أوضح بشكل أنه نطلع من المحاضرة هذه
+
+207
+00:15:45,010 --> 00:15:48,330
+فاهمين أن ال pathogen في منه absolute دايما
+
+208
+00:15:48,330 --> 00:15:49,570
+pathogen في منه
+
+209
+00:15:53,490 --> 00:15:57,330
+أقرب إلى ال pathogen أو أقرب إلى ال non pathogen
+
+210
+00:15:57,330 --> 00:16:02,130
+إذا وصلنا لهذا المفهوم بنرتاح كتير في المستقبل ال
+
+211
+00:16:02,130 --> 00:16:05,350
+pathogen هو اللي بيسبب infection ال non اللي
+
+212
+00:16:05,350 --> 00:16:08,250
+بيسببش ال opportunistic اللي بيسببش اللي لاجل عند
+
+213
+00:16:08,250 --> 00:16:12,090
+الناس اللي عندهم جاذب منها إيه ضعيفة منها انتهازية
+
+214
+00:16:12,090 --> 00:16:16,330
+opportunistic pathogenواللي بتسبب عند ال
+
+215
+00:16:16,330 --> 00:16:19,510
+immunocompromised immunocompromised عكسها
+
+216
+00:16:19,510 --> 00:16:25,230
+immunocompetent إيه
+
+217
+00:16:25,230 --> 00:16:28,670
+يعني competent؟ فعليه الجيهة اللي بناها عنده فعليه
+
+218
+00:16:28,670 --> 00:16:29,990
+immunocompromised
+
+219
+00:16:33,260 --> 00:16:37,500
+الـ Pathogenicity هي قدرة الكائن المعدى الـ
+
+220
+00:16:37,500 --> 00:16:43,520
+infectious agent to cause disease طبعا قدرته كمان
+
+221
+00:16:43,520 --> 00:16:47,260
+مرة ما احنا قولناه بشوية ان فيه اختلافات هي واحد ب
+
+222
+00:16:47,260 --> 00:16:50,920
+.. بmicrobe واحد والتاني ب .. بمليون microbe عشان
+
+223
+00:16:50,920 --> 00:16:55,740
+يعمل infection ففيه علاقة بالـ virulence ال
+
+224
+00:16:55,740 --> 00:16:57,480
+virulence .. ال virulence
+
+225
+00:17:00,140 --> 00:17:03,080
+للpathogenicity، سنين pathogen، لكن واحد viralness
+
+226
+00:17:03,080 --> 00:17:07,280
+تبعه أعلى من مين؟ من التاني، ما الذي حدد أن ال
+
+227
+00:17:07,280 --> 00:17:10,460
+viralness بتبع هذا الميكروب أعلى من الآخر؟ وجود
+
+228
+00:17:10,460 --> 00:17:16,060
+عدد من ال viralness factors، فاكرين ال viralness
+
+229
+00:17:16,060 --> 00:17:21,470
+factors؟ ال flagellaفيرولينز فاكتور الاندوتكسين
+
+230
+00:17:21,470 --> 00:17:25,090
+فيرولينز فاكتور الكبسول فيرولينز فاكتور ال
+
+231
+00:17:25,090 --> 00:17:29,390
+بالايتات الاتتشمنت فيرولينز فاكتور بعض الانزيم ال
+
+232
+00:17:29,390 --> 00:17:36,090
+spores كتير من التراكيب سميناها ايش؟ اي
+
+233
+00:17:36,090 --> 00:17:44,530
+شيء بيزيد من قدرة البكتيريا على ال survival فهو
+
+234
+00:17:44,530 --> 00:17:49,920
+فيرولينز فاكتور الآن البكتيريا هجمتالبكتيريا أوذيت
+
+235
+00:17:49,920 --> 00:17:54,040
+فتحولت من vegetative إلى sport، ال sport is more
+
+236
+00:17:54,040 --> 00:17:58,520
+resistant، صح؟ وبالتالي هيكون قتلها صعب، روح الخطر
+
+237
+00:17:58,520 --> 00:18:01,560
+بتعود ترجع مرة أخر، وبالتالي تعتبر virulence
+
+238
+00:18:01,560 --> 00:18:08,780
+factor الفاكرين الكلمة جرعة؟ لص، قولنا أننا زمك؟
+
+239
+00:18:08,780 --> 00:18:15,140
+هدف مين؟في ال disinfectants و في ال antiseptics
+
+240
+00:18:15,140 --> 00:18:18,380
+اللي استخدمناهم في المحاضرة السابقة احنا الآن ال
+
+241
+00:18:18,380 --> 00:18:23,720
+dose ان هنا بدنا ننظرلها بنظرة شوية مختلفة تسمعوا
+
+242
+00:18:23,720 --> 00:18:26,720
+بال lethal dose و ال infectious dose و ده أول مرة
+
+243
+00:18:26,720 --> 00:18:34,040
+مصنعتها لسه طيب ال LD50 و ال ID50 خليكم معايا دي
+
+244
+00:18:34,040 --> 00:18:40,670
+بتمركب أنا و دي بت meet farأعطيت ال meat fire جرعة
+
+245
+00:18:40,670 --> 00:18:49,670
+X ماتوا خمسين وظلوا خمسين الجرعة X هذه بسميها LD50
+
+246
+00:18:49,670 --> 00:18:56,290
+lethal dose اللي قتلت نصف عدد حيانة تجارب LD50
+
+247
+00:18:56,290 --> 00:18:59,350
+طبعا
+
+248
+00:18:59,350 --> 00:19:01,850
+احنا ده .. ده .. ده .. ده .. ده .. ده .. ده .. ده
+
+249
+00:19:01,850 --> 00:19:02,650
+.. ده .. ده .. ده .. ده .. ده .. ده .. ده .. ده ..
+
+250
+00:19:02,650 --> 00:19:05,980
+ده ..بيروح تابعون الصيدرة ب .. ب .. لازم يكونوا
+
+251
+00:19:05,980 --> 00:19:09,580
+عارفين عشان يحددوا ماذا سميت ال drugs وبيوصفوا ال
+
+252
+00:19:09,580 --> 00:19:14,480
+drugs بال LD50 تبعهم كل ما قالت .. كل ما قلت ال
+
+253
+00:19:14,480 --> 00:19:19,240
+LD50 كرقم تمام كل ما كان العامل بيكون more toxic
+
+254
+00:19:19,240 --> 00:19:25,860
+more toxic يعني مثلا نقول انه ال LD50 point zero
+
+255
+00:19:25,860 --> 00:19:29,760
+zero zero مثلا five grams ولا point five gram مين
+
+256
+00:19:29,760 --> 00:19:35,000
+أكتر سمية؟الـ point zero zero zero صح؟ لما ال ID
+
+257
+00:19:35,000 --> 00:19:41,300
+50 تكون رقم صغير الحياة بتكون بأكثر صميمة ال ID 50
+
+258
+00:19:41,300 --> 00:19:47,400
+بدل ما هو قتل إحداث إصابة infection إحداث إصابة
+
+259
+00:19:47,400 --> 00:19:54,020
+يعني هي الجرعة اللازمة لإحداث إصابة in fifty
+
+260
+00:19:54,020 --> 00:19:55,860
+percent of the test animal
+
+261
+00:20:02,160 --> 00:20:04,960
+الدراجز بقى هتكلم عن concentration، في البكتيري
+
+262
+00:20:04,960 --> 00:20:08,700
+هتكلم عن إيش؟ عن number، في البكتيري هتكلم عن
+
+263
+00:20:08,700 --> 00:20:11,580
+number، ممكن تقولوا على ال number concentration
+
+264
+00:20:11,580 --> 00:20:17,220
+برضه، و ده لأ، خمس خلايا في كل milli، من هنا تبقى
+
+265
+00:20:17,220 --> 00:20:19,960
+تبقى ريانيه concentration، بس لكن في الآخر هنقول
+
+266
+00:20:19,960 --> 00:20:23,890
+عنه numberماذا هو الفرق بين ال ID 50 و ال ID 50؟
+
+267
+00:20:23,890 --> 00:20:29,810
+ال ID 50 هي نسبة القتل ل 50% بينما ال ID 50 هي
+
+268
+00:20:29,810 --> 00:20:33,430
+نسبة إحداث ال infection ب 50% بس أنا بدأ أرجع
+
+269
+00:20:33,430 --> 00:20:37,430
+برجوع وأعرض
+
+270
+00:20:37,430 --> 00:20:40,210
+اللوحة هذه اللي قدامنا هذه اللوحة اللي قدامنا بدنا
+
+271
+00:20:40,210 --> 00:20:46,320
+نطلع عليها من منظور كتير كويساحنا عايشين الآن في
+
+272
+00:20:46,320 --> 00:20:50,820
+مجتمع وفي المجتمع فينا normal flora معينة، normal
+
+273
+00:20:50,820 --> 00:20:54,850
+flora معينةلو نبنا في المستشفى لأي غراب من الأغراض
+
+274
+00:20:54,850 --> 00:20:58,090
+و كلمة نبنا في المستشفى مش ضرورى أنها مرض معدي
+
+275
+00:20:58,090 --> 00:21:02,070
+ممكن تكون إيدنا انكسرة صح ولا عراب لا حادث حارق
+
+276
+00:21:02,070 --> 00:21:06,950
+أوى فبقولنا أن مكوثنا في المستشفى قد يغير من ال
+
+277
+00:21:06,950 --> 00:21:09,310
+resident flora لحظة اللورة الأحمر اللى مولودة في
+
+278
+00:21:09,310 --> 00:21:13,710
+الجهة التانية مايكروبات ماكانت موجودة في الجهة هذه
+
+279
+00:21:13,710 --> 00:21:19,250
+أو زيادت هذه الميكروبات أو بقيت كما هي لكن تغيرت
+
+280
+00:21:19,250 --> 00:21:24,520
+طبيعتهاإيش بقيت كمية و تغيرت طبيعتها؟ أنا أبقى
+
+281
+00:21:24,520 --> 00:21:30,120
+أعطي مثال كتير سهل، بس بعد ما نخلص المثال، ننساه و
+
+282
+00:21:30,120 --> 00:21:36,080
+ننتقل للحقيقة الفقران اللي في المنازل اللي بتكون
+
+283
+00:21:36,080 --> 00:21:42,020
+عايشة في معلبة الأدمين صعب جدا صيدها، لكن لو كانت
+
+284
+00:21:42,020 --> 00:21:46,480
+فقرا برية و حطينا لها فخ بتلاقي اتجاه بسهولة، سبب؟
+
+285
+00:21:47,240 --> 00:21:54,640
+إن الفقران في المنزل أحنا أعداء لها فهي تعلمت
+
+286
+00:21:54,640 --> 00:22:00,920
+تقنيات البقاءأمامنا، أمام الخطر الدهن من البني
+
+287
+00:22:00,920 --> 00:22:04,760
+أدمين، من البشر، بس في البر احنا مافيش احتجاج
+
+288
+00:22:04,760 --> 00:22:08,960
+بيننا وبينها، وبالتالي اتعلمت ممكن كيف تحمي نفسها
+
+289
+00:22:08,960 --> 00:22:12,860
+من الطيور الجارحة، من بعض الحيوانات الأخرى، لكن ما
+
+290
+00:22:12,860 --> 00:22:16,780
+تعلمت كيف تحمي نفسها من الإنسان، فبنلجي الفار
+
+291
+00:22:16,780 --> 00:22:20,520
+بالفعل، ليه صار لإيه ممكن الفار في المنزل؟ لأنه
+
+292
+00:22:20,520 --> 00:22:27,310
+اتعلم من ال ..من ال .. من الأدمين بينما هدك ضاله
+
+293
+00:22:27,310 --> 00:22:32,170
+سادش تمام لما اتعلم طب تعالوا نطبق الموضوع على
+
+294
+00:22:32,170 --> 00:22:36,590
+البكتيريا البكتيريا في المجتمع و البكتيريا في
+
+295
+00:22:36,590 --> 00:22:39,990
+المستشفى في المستشفى بنستخدم أطنان من المضادة
+
+296
+00:22:39,990 --> 00:22:45,230
+الحيويةبنى فى المجتمع لاستخدام اقل فالان البكتيريا
+
+297
+00:22:45,230 --> 00:22:50,190
+فى المستشفيات تعلمت كيف تقاوم المناضات الحيوية كما
+
+298
+00:22:50,190 --> 00:22:54,850
+.. كما تعلم الفقر تماما فى المنزل أساليب ومكر
+
+299
+00:22:54,850 --> 00:22:59,190
+البشر ضدهفصارت more resistant في المستشفيات
+
+300
+00:22:59,190 --> 00:23:02,450
+البكتيريا than the community، إذا الآن من حجي انا
+
+301
+00:23:02,450 --> 00:23:06,810
+إذا انا اكتسبت عدوى من المستشفى أقول إنها والله
+
+302
+00:23:06,810 --> 00:23:11,110
+هادى خاصة من المستشفى لإنه البكتيرى المثابرة
+
+303
+00:23:11,110 --> 00:23:14,530
+بقالها وإن كان اسمها Aesthesia coli هتختلف عن
+
+304
+00:23:14,530 --> 00:23:17,790
+Aesthesia coli في من؟ في المجتمع، لذا قسموا ال
+
+305
+00:23:17,790 --> 00:23:20,590
+infection إلى نوعين، community acquired من
+
+306
+00:23:20,590 --> 00:23:23,610
+المجتمع، من الجامعة، من الشارع، من البيت، من أي
+
+307
+00:23:23,610 --> 00:23:28,160
+حاجةلكن مش المستشفى والنقطة التانية من المستشفى
+
+308
+00:23:28,160 --> 00:23:34,000
+واسمها hospital acquired أو nosocomial والاسم
+
+309
+00:23:34,000 --> 00:23:39,140
+الحديث هي ثلاث أسامي الان health care associated
+
+310
+00:23:39,140 --> 00:23:45,080
+infections H A I health care associated infections
+
+311
+00:23:45,080 --> 00:23:48,400
+ليش سميناها health care و بطلنا عن hospital
+
+312
+00:23:48,400 --> 00:23:54,190
+infectionالان ال hospital هو مكان بيكون فيه رعاية
+
+313
+00:23:54,190 --> 00:23:57,630
+طبية صح ولا لا؟ رعاية طبية، طب هل هو الوحيد اللي
+
+314
+00:23:57,630 --> 00:24:01,490
+بيقدم رعاية طبية ومن ضمنها مغادرة هاوية؟ أكيد لأ،
+
+315
+00:24:01,490 --> 00:24:05,590
+دور العجزة .. دور العجزة بتقدم رعاية طبية،
+
+316
+00:24:05,590 --> 00:24:09,550
+الحضانات ال nurseries بتقدم رعاية طبيةقد يكون
+
+317
+00:24:09,550 --> 00:24:14,770
+اماكن للمدمنين بتجدم رعاية طبية اذا اي مؤسسة اللي
+
+318
+00:24:14,770 --> 00:24:17,770
+لها علاقة بال health care ايه الجبن اللي ميزها عن
+
+319
+00:24:17,770 --> 00:24:21,270
+مين؟ عن المؤسسات اللي ماعناش علاقة بال health care
+
+320
+00:24:21,270 --> 00:24:25,030
+اذا ال community acquired ال hospital acquired
+
+321
+00:24:25,030 --> 00:24:30,490
+التصنيف سببه واضح ان اي microbe يكتسب المستشفى
+
+322
+00:24:30,490 --> 00:24:34,730
+غالبا يكون اسوأ من اللي هو ال microbe انا بدي
+
+323
+00:24:34,730 --> 00:24:39,410
+اعطاكم بس مثال بسيطالمجاري الـ waste water اللي
+
+324
+00:24:39,410 --> 00:24:44,370
+خارج من المستشفى غير اللي خارج من المنازلو في
+
+325
+00:24:44,370 --> 00:24:47,110
+دراسة اتراينها يمكن جابلت سبع سنوات او تمان سنوات
+
+326
+00:24:47,110 --> 00:24:51,530
+في الجامعة وجدنا فروقات كبيرة في ال resistant rate
+
+327
+00:24:51,530 --> 00:24:55,010
+لنفس البكتيريا يعني مثلا أخدنا E.coli و E.coli
+
+328
+00:24:55,010 --> 00:24:57,930
+أسينيتوباكتر و أسينيتوباكتر سوداموناس و سوداموناس
+
+329
+00:24:57,930 --> 00:25:02,430
+وجدنا resistance عالي لذلك يجب على المستشفيات أن
+
+330
+00:25:02,430 --> 00:25:06,610
+تقوم بمعالغة المياه العادمة تبعتها داخليا في
+
+331
+00:25:06,610 --> 00:25:11,400
+المستشفيات قبل ضقة إلى المجتمعيبقى تصنيف هذا
+
+332
+00:25:11,400 --> 00:25:15,080
+question مش كويس الميزة مع بعض ونكمل ان شاء الله
+
+333
+00:25:15,080 --> 00:25:16,400
+المرة القادمة بإذن الله نفس الامور
+
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/j3TMld9XqFY_raw.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/j3TMld9XqFY_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..82f75b47277bf5d565fea8703c625ebee8780f8f
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/j3TMld9XqFY_raw.srt
@@ -0,0 +1,1648 @@
+1
+00:00:20,590 --> 00:00:24,930
+بسم الله الرحمن الرحيم استكمل ان شاء الله موضوع
+
+2
+00:00:24,930 --> 00:00:28,990
+الـ Gram Negative Bacilli الـ Enterobacteriaceae
+
+3
+00:00:28,990 --> 00:00:35,070
+بدأنا بالمشهورة الاشريشيا كولاي تحدثنا عن
+
+4
+00:00:35,070 --> 00:00:37,770
+الاشريشيا كولاي على إنها normal microbiota of the
+
+5
+00:00:37,770 --> 00:00:41,070
+intestinal tract و تحدثنا عنها إنها outside the
+
+6
+00:00:41,070 --> 00:00:44,110
+intestinal tract is apoptotic و هي المثال عليها
+
+7
+00:00:44,110 --> 00:00:46,770
+اللي قُلتها على اللوحة إنها تعمل urinary tract
+
+8
+00:00:46,770 --> 00:00:49,170
+infection بالعكس هي number one cause of urinary
+
+9
+00:00:49,170 --> 00:00:49,990
+tract infection
+
+10
+00:01:01,000 --> 00:01:08,040
+الان انا لما اخدت عينة اليوران فى المعمل وقررت انه
+
+11
+00:01:08,040 --> 00:01:12,400
+positive positive يعني فى growth لبكتيريا وكانت
+
+12
+00:01:12,400 --> 00:01:15,420
+ايشريشيا كولاي كان ي differentiate between
+
+13
+00:01:15,420 --> 00:01:19,800
+cystitis and pyronephritisبتقدروا تمييزوا بينت
+
+14
+00:01:19,800 --> 00:01:23,820
+انت؟ صعب جدا لنميز بينت انت من عينة UL المصلطة على
+
+15
+00:01:23,820 --> 00:01:27,120
+المختبر، واحنا ماعناش معلومات إلا إن هو عينة UL،
+
+16
+00:01:27,120 --> 00:01:29,940
+لأنه إذا كان بيوليوفريتس أو سيستريتس، هتطلع
+
+17
+00:01:29,940 --> 00:01:30,900
+العيناها
+
+18
+00:01:33,200 --> 00:01:38,260
+لأ، بدى اوضح نقطة القاعدة هنا، انه في limitations،
+
+19
+00:01:38,260 --> 00:01:43,120
+ايه ال limitations؟ يعني حدود، محددات لحت رتائبنا
+
+20
+00:01:43,120 --> 00:01:46,920
+في المزرعة، البعض مننا بيظن انه إذا عزل مايكروب،
+
+21
+00:01:46,920 --> 00:01:50,600
+فهو هذا هو المسبب لل infection، إذا عزل مايكروب،
+
+22
+00:01:50,600 --> 00:01:53,920
+فهو هذا هو ال disease، لأ الحقيقة أن هناك عامل أخر
+
+23
+00:01:53,920 --> 00:01:58,280
+لازم نظر إليها بالاتباع، مثلاللتفريق ما بين
+
+24
+00:01:58,280 --> 00:02:02,500
+cestitis و pyruhnephritis نضطر نعمل فحص اسمه CRP
+
+25
+00:02:02,500 --> 00:02:05,820
+اللي وزير active protein هتكلم عنه في مرحلة أخر في
+
+26
+00:02:05,820 --> 00:02:06,940
+ال micro pyruh
+
+27
+00:02:18,470 --> 00:02:21,890
+كانت المثال التانى على ان الاشريشيا كوراي outside
+
+28
+00:02:21,890 --> 00:02:24,550
+the intestinal tract هي pathogen السبسيز و ال
+
+29
+00:02:24,550 --> 00:02:27,770
+meningitis و خاصة في ال neonatal و برجه ذكرنا
+
+30
+00:02:27,770 --> 00:02:30,970
+معلومة ان هي number one cause of neonatal
+
+31
+00:02:30,970 --> 00:02:35,250
+meningitis بس أكتنا على حقيقة ان ال strains اللى
+
+32
+00:02:35,250 --> 00:02:40,130
+بتسبب neonatal meningitis غالبا بتكون capsulated و
+
+33
+00:02:40,130 --> 00:02:43,050
+عندها K1 انتجين و اللى بيقول عنه هو ال capsular
+
+34
+00:02:43,050 --> 00:02:48,460
+antigenالان انتقلنا لمرحلة انهم outside the
+
+35
+00:02:48,460 --> 00:02:50,500
+intestinal tract وانتقلنا لقاله mainly ل inside
+
+36
+00:02:50,500 --> 00:02:53,480
+the intestinal tract احنا اتفقنا انه في اربع من ال
+
+37
+00:02:53,480 --> 00:02:57,300
+bacterial C احكي التلاتة الاولين فاكر اني اقول
+
+38
+00:02:57,300 --> 00:03:02,640
+اشريشيا كولاي وقلنا سالمونيلا شيجيلا ويرسينيا هدول
+
+39
+00:03:02,640 --> 00:03:04,960
+التلاتة they are not normal in the intestinal
+
+40
+00:03:04,960 --> 00:03:07,700
+tract they cause intestinal tract infection ضفنا
+
+41
+00:03:07,700 --> 00:03:10,880
+اليهم في المرة التانية الاشريشيا كولاي وقلنا some
+
+42
+00:03:10,880 --> 00:03:13,180
+strains مش كلهم some strains
+
+43
+00:03:17,960 --> 00:03:21,780
+طب نحط شق أمام ال E cherisha coli مثلا قدامي على
+
+44
+00:03:21,780 --> 00:03:27,200
+لوحة بالبول انترو فاطو جنك E cherisha coli طبعا
+
+45
+00:03:27,200 --> 00:03:32,580
+هذا الاسم خادع الصحيح و بدنا نفكس عليه شوية من أي
+
+46
+00:03:32,580 --> 00:03:36,680
+ناحية خادع انه بيعطيني حق ان انا بتكلم عن كل ال E
+
+47
+00:03:36,680 --> 00:03:41,150
+cherisha coliاللي بتعمل infection في ال intestine
+
+48
+00:03:41,150 --> 00:03:44,830
+لإن ال pathogenic كان ممرضا لكن وقع الأمر هو
+
+49
+00:03:44,830 --> 00:03:49,390
+المصطلح اخترع للتعبير عن ما أنا قلته الآن لكن
+
+50
+00:03:49,390 --> 00:03:54,370
+لجينا أصناف مختلفة فخلينا واحد من الأصناف بالاسم
+
+51
+00:03:54,370 --> 00:03:57,530
+نفسه enteropathogenic واخترعنا أسماء جديدة من
+
+52
+00:03:57,530 --> 00:04:01,490
+الأسماء لأنواع أخرى هنشوفهم كمان شوية ف
+
+53
+00:04:01,490 --> 00:04:08,070
+enteropathogenic-epec هي أحد الأصناف و ليس كل
+
+54
+00:04:08,070 --> 00:04:13,490
+الأصنافتعمل fever, infandiaria, vomiting, nausea,
+
+55
+00:04:14,030 --> 00:04:17,610
+non bloody diarrhea, non bloody stool يعني ال
+
+56
+00:04:17,610 --> 00:04:23,430
+stool مش مدمّم، مافيش فيه، مش مصحوب بيه دم ناتج
+
+57
+00:04:23,430 --> 00:04:28,230
+غالبا عن destruction of surface microvilli تركزوا
+
+58
+00:04:28,230 --> 00:04:32,370
+معاه و تذكروا ال intestinal tract ال intestinal
+
+59
+00:04:32,370 --> 00:04:36,170
+tract فيها ال villi و ال villi هي المسؤولة عن ال
+
+60
+00:04:36,170 --> 00:04:38,790
+absorption لل digested food
+
+61
+00:04:45,880 --> 00:04:49,000
+طب انا ماتوقعش ان يكون destruction كامل لكل ال
+
+62
+00:04:49,000 --> 00:04:52,060
+microbial فبيقل ال absorption لما يقل ال
+
+63
+00:04:52,060 --> 00:04:58,220
+absorption ايش بيزيد في ال intestine؟ الأملاح،
+
+64
+00:04:58,220 --> 00:05:01,860
+فاكرهين؟ فاكرهين دي يعني إذا زاد الأملاح، لازم
+
+65
+00:05:01,860 --> 00:05:09,070
+النسم يخففها، ده نائزالمتعارف عليها في إدارة لكن
+
+66
+00:05:09,070 --> 00:05:12,450
+الأسباب بتكون متنوعة لماذا لما تم الامتصاص أو
+
+67
+00:05:12,450 --> 00:05:16,570
+لماذا تم التراكم بتكون أسباب مختلفة فهذه سببها ان
+
+68
+00:05:16,570 --> 00:05:20,910
+ال micro-ply اللي موجودة مادت كيف مادت؟ عن طريق
+
+69
+00:05:20,910 --> 00:05:25,390
+attachment بنوع خاص من ال ply بيسمونه bundle
+
+70
+00:05:25,390 --> 00:05:32,280
+forming bundle معناها حزمة bundle forming plyأو
+
+71
+00:05:32,280 --> 00:05:37,400
+البكتيريا بتعمل stimulation لل Intracellular
+
+72
+00:05:37,400 --> 00:05:42,460
+Calcium Level و rearrangement لل Intracellular
+
+73
+00:05:42,460 --> 00:05:42,900
+Actin
+
+74
+00:05:45,540 --> 00:05:48,060
+اللي أنا المرة الماضية قولتلكم عنها ال tide 3
+
+75
+00:05:48,060 --> 00:05:51,080
+secretory system قولتلكم اتفرج عليها على اليوتيوب
+
+76
+00:05:51,080 --> 00:05:55,480
+ان البكتيريا بتعمل injection لبعض ال proteins ال
+
+77
+00:05:55,480 --> 00:06:00,300
+proteins هتعمل modulation او modification للأكتين
+
+78
+00:06:00,300 --> 00:06:04,880
+بحيث انها تصبح انها ايه انترال سيلولار فابو سيترسس
+
+79
+00:06:04,880 --> 00:06:08,620
+يعني تاخد البكتيريا فمعظم ال
+
+80
+00:06:16,260 --> 00:06:21,700
+أخر من البكتيري اسمه Etec
+
+81
+00:06:21,700 --> 00:06:28,080
+اختلف عنها الأولنية بحرف ال P معنى T تتذكر
+
+82
+00:06:28,080 --> 00:06:30,920
+الكوليرة؟ اذا كنت تتذكر الكوليرة، مدام الterm
+
+83
+00:06:30,920 --> 00:06:35,060
+اليوم، what is the mechanism of action للكوليرة؟
+
+84
+00:06:35,060 --> 00:06:36,200
+كيف الامتحان؟
+
+85
+00:06:40,050 --> 00:06:43,710
+اللي بتعمل ال .. برضه بتزيد في رجلات الأمناح برضه
+
+86
+00:06:43,710 --> 00:06:47,870
+.. كيف؟ و أنا .. أنا السؤال كيف؟ الادمين يتطلب الـ
+
+87
+00:06:47,870 --> 00:06:52,990
+A1، يتطلب عن الأديولات الـ A6، بتعمل ال activation
+
+88
+00:06:54,570 --> 00:06:58,850
+فبتحول ال .. بتحول ال advanced cyclase بيحول ال
+
+89
+00:06:58,850 --> 00:07:05,050
+ATP لسجن ال EMB لسجن ال EMB بيزود .. بيحيم ال إثاث
+
+90
+00:07:05,050 --> 00:07:08,610
+لل electrolytes حتي تسيط كميةها، حتي تخفيفها
+
+91
+00:07:08,610 --> 00:07:12,410
+الشثمة من ال water ده ال ice blockers، ده ال ice
+
+92
+00:07:12,410 --> 00:07:14,970
+blockers، هاي .. هاي بالفعل، هاي ال story نفس
+
+93
+00:07:14,970 --> 00:07:18,510
+القصة، عارفين ليش نفس القصة؟ لإن أنا هتكلم عن
+
+94
+00:07:18,510 --> 00:07:22,690
+تكسين، ال action تبعه، بنوصف واحد كبرالايت تكسين
+
+95
+00:07:23,410 --> 00:07:26,310
+الكوليراي تكسينه بتاعة الانتروتوكشينك إيشريشيا
+
+96
+00:07:26,310 --> 00:07:30,070
+كوليراي will produce دياليا similar to that of
+
+97
+00:07:30,070 --> 00:07:33,650
+Vibrio cholera نتكلم شوية عن Vibrio cholera لإنه
+
+98
+00:07:33,650 --> 00:07:36,090
+مهم جدا لإذا عرفنا المعلومات اللي علي Vibrio
+
+99
+00:07:36,090 --> 00:07:37,710
+cholera هي نفس المعلومات اللي علي الانتروتوكشينك
+
+100
+00:07:37,710 --> 00:07:43,010
+إيشريشيا كولرا الدياليا احنا قلنا جاب اللوحة ان
+
+101
+00:07:43,010 --> 00:07:47,490
+الستول is not as shared with blood مافي شي مش مدمم
+
+102
+00:07:57,110 --> 00:08:00,390
+أي دياريا في ال rice water لما بنغسل الرز أشبه كله
+
+103
+00:08:00,390 --> 00:08:05,430
+المياه ميليكي و whiteish صح ولا لأ؟ هناك
+
+104
+00:08:05,430 --> 00:08:09,050
+resemblance ما بين الدياريا و ما بين ال rice water
+
+105
+00:08:09,050 --> 00:08:13,610
+stool لذلك لما نتقف الناس في حالة الأوبع بنقولهم
+
+106
+00:08:13,610 --> 00:08:17,570
+أن أي دياريا في حالة الوباعة أي دياريا هي
+
+107
+00:08:17,570 --> 00:08:21,130
+suspected to be as a result of vibrio cholera
+
+108
+00:08:26,750 --> 00:08:33,830
+Lose stool per day لكن لما يكون في رايس water
+
+109
+00:08:33,830 --> 00:08:41,440
+stool هذا مدعاة لمش حقعالى الطبيب ان يعالج المريض
+
+110
+00:08:41,440 --> 00:08:46,600
+على انه كوليرة قبل ما يبدأ .. قبل ما يعني يكون فيه
+
+111
+00:08:46,600 --> 00:08:49,520
+تشخيص نهائي يبقى بيصير فيه ان انا اتقال ان هو
+
+112
+00:08:49,520 --> 00:08:52,660
+المريض اندو كوليرة ليه احنا مهتمين باننا نعالج
+
+113
+00:08:52,660 --> 00:08:57,300
+المريض بسرعة من جانب الجهد التشخيص لحاجتين لمصلحة
+
+114
+00:08:57,300 --> 00:09:00,640
+المريض نفسه ان احنا بدنا .. لان ال .. ال .. ال ..
+
+115
+00:09:00,640 --> 00:09:01,020
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+116
+00:09:01,020 --> 00:09:01,360
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+117
+00:09:01,360 --> 00:09:01,820
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+118
+00:09:01,820 --> 00:09:02,100
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+119
+00:09:02,100 --> 00:09:04,340
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+120
+00:09:04,340 --> 00:09:04,520
+.. ال
+
+121
+00:09:08,550 --> 00:09:13,590
+والمسألة التانية عشان خاطر المجتمع هذا الشخص
+
+122
+00:09:13,590 --> 00:09:18,650
+المصاب يشكل reservoir للبكتيريا فمن واجب انا ادور
+
+123
+00:09:18,650 --> 00:09:23,110
+عليهم و اعالجهم يعني حتى العائلة اللي بيكون فيها
+
+124
+00:09:23,110 --> 00:09:26,670
+فرد اصيب غالبا لازم اعملهم quarantine quarantine
+
+125
+00:09:26,670 --> 00:09:30,810
+يعني حجز حجب الصحي عشان انا اطلع مين منهم ممكن
+
+126
+00:09:30,810 --> 00:09:33,070
+يكون exposed او ممكن اعطيه
+
+127
+00:09:36,940 --> 00:09:41,720
+إذا احنا معنين لحظة rice water stool معنوعة صغيرة
+
+128
+00:09:41,720 --> 00:09:45,860
+طبعا استخدمناها في نشر الوعي في حالة اللي هو
+
+129
+00:09:45,860 --> 00:09:51,060
+الأوبيا مش بس الطبيب بيقصر او بتخلع ال protocol في
+
+130
+00:09:51,060 --> 00:09:53,680
+حالة الأوبيا احنا كمان بتخلع ال protocol في
+
+131
+00:09:53,680 --> 00:09:57,440
+المختبر كيف نتخلع ال protocol؟ هعطيكم مثل الكورية
+
+132
+00:09:57,440 --> 00:10:03,320
+نفسيقررنا عينة زرعناها بعد 24 ساعات تطلع لمستعملها
+
+133
+00:10:03,320 --> 00:10:08,360
+صح؟ بعد 24 ساعات نطلع عليها ساكروس فرمنتر من طول
+
+134
+00:10:08,360 --> 00:10:11,280
+مش عارف شوه نعملها biochemical test كمان 24 ساعات
+
+135
+00:10:11,280 --> 00:10:17,060
+صح؟ هجدت صار الآن 48 ساعات و 24 ساعات أخرى
+
+136
+00:10:17,060 --> 00:10:21,620
+لستيرولوجي زائد ال sensitivity testing هي 72 ساعات
+
+137
+00:10:21,620 --> 00:10:25,310
+كاملةهل من المنطق ان في حالة الأوبيا انتظر 48
+
+138
+00:10:25,310 --> 00:10:29,370
+ساعة؟ طب انا اتقال كيف باكسل ال protocol بعد 24
+
+139
+00:10:29,370 --> 00:10:32,510
+ساعة المستعمرة بتنمو Suspected Colonist ايه يعني
+
+140
+00:10:32,510 --> 00:10:35,110
+Suspected Colonist؟ مشترك بيها، يلها مواصفات
+
+141
+00:10:35,110 --> 00:10:38,210
+معينة، بدل من بعملها biochemical test تجارب 24
+
+142
+00:10:38,210 --> 00:10:42,770
+ساعة، بعملها فحص serology واحد مباشر او فحصين one
+
+143
+00:10:42,770 --> 00:10:45,130
+minute test one minute test يعني ما بيستخدموا
+
+144
+00:10:45,130 --> 00:10:50,390
+معايا طويلة فباقتصر من ال diagnosis حوالي 48 ساعة
+
+145
+00:10:51,370 --> 00:10:54,790
+هذا لايعني ان انا لا اكمل إجراءات روتينية لكن انا
+
+146
+00:10:54,790 --> 00:10:58,470
+بهذا الحال بعمل تقريرين تقرير مبدئي وتقرير نهائي
+
+147
+00:10:58,470 --> 00:11:03,010
+حتى اوفر وقت لان احنا في حالة غير طبيعية اسمها
+
+148
+00:11:03,010 --> 00:11:08,130
+ability مكتر، لقيت فرازي ان هي ال electrolyte
+
+149
+00:11:08,130 --> 00:11:15,490
+مابنحلش الا بالناء يعني بالواتر يعني نتخلص من كمية
+
+150
+00:11:15,490 --> 00:11:20,290
+ال electrolyte بالناء صحيحيعني هينتج المشكلة
+
+151
+00:11:20,290 --> 00:11:24,590
+تانية؟ ما هو طبعا مشكلتين هينتج، حاسس ان هناك نقص
+
+152
+00:11:24,590 --> 00:11:28,610
+في المياه و نقص في الالكتروليات، لذلك اي قيمة
+
+153
+00:11:28,610 --> 00:11:34,640
+لبيارية should include two measureswater
+
+154
+00:11:34,640 --> 00:11:38,160
+replacement اللي هو الـrehydration وعالطريق المحلي
+
+155
+00:11:38,160 --> 00:11:42,260
+و ال electrolyte بتسمعوا بالمصريين .. أخوان
+
+156
+00:11:42,260 --> 00:11:46,040
+المصريين اللاطفين كان عندهم دعاية مشهورة لمعالجة
+
+157
+00:11:46,040 --> 00:11:52,700
+.. كان اسمه محلول معالجة الجفاف و جعلوا يمكن سنين
+
+158
+00:11:52,700 --> 00:11:59,540
+يعملوا عملية تسويق لهذا المنتج على التلفزيون لأنه
+
+159
+00:11:59,540 --> 00:12:01,660
+ديارية في العالم التالت
+
+160
+00:12:06,060 --> 00:12:08,880
+هو قاطر رقم واحد وبالتالي احنا مضطرين بالفعل
+
+161
+00:12:08,880 --> 00:12:13,320
+نعالجه بانه نرجعله المي اللي فقدها ونرجعله اللي هو
+
+162
+00:12:13,320 --> 00:12:17,100
+ال electrolyte اللي فقدها فجزء مهم جدا البعض
+
+163
+00:12:17,100 --> 00:12:21,100
+بيعتقد ان إيقاف الديارية هو أمر مهم لإيقاف
+
+164
+00:12:21,100 --> 00:12:25,540
+الديارية البعض بيظن انه غير مهم وبالعكس قد يؤذي
+
+165
+00:12:25,540 --> 00:12:31,490
+المرضلذلك لا تستخدم الانفيا النهار اسمها ايموديوم
+
+166
+00:12:31,490 --> 00:12:34,590
+معروف عن ان هو هذا بيوقف حركة الدودة .. الحركة
+
+167
+00:12:34,590 --> 00:12:37,790
+الدودية اللي امها هو هذا محدد ببتاعته خلال نص ساعة
+
+168
+00:12:37,790 --> 00:12:41,470
+وجه في الإسهال فبقى إذا أحنا متفقين إن الإسهال هو
+
+169
+00:12:41,470 --> 00:12:44,950
+reaction طبيعي من الدسم التخلص من ال micro
+
+170
+00:12:44,950 --> 00:12:48,690
+organism فبالتالي إذا وقفنا الإسهال بطريقة هذه
+
+171
+00:12:48,690 --> 00:12:53,380
+الميكروب والتوكسين هيبقى له موجودلكن الأفضل اللي
+
+172
+00:12:53,380 --> 00:12:57,240
+ما حكينا إن السوائل والإلكتروبات اللي بتفقدها
+
+173
+00:12:57,240 --> 00:13:01,660
+نعوضها والجانب التاني ان يعالج العامل المسبب نتخلص
+
+174
+00:13:01,660 --> 00:13:05,260
+منه إذا كان بالبروكلية إذا كان سالمونيلا إذا كان
+
+175
+00:13:05,260 --> 00:13:08,020
+إشريشة إذا كان شجلة إذا كان انتميبة أو whatever
+
+176
+00:13:08,020 --> 00:13:12,620
+causes it احنا خرجنا عن النص كتير لكن كله عشان
+
+177
+00:13:12,620 --> 00:13:13,620
+نوضع نقطة واحدة
+
+178
+00:13:21,760 --> 00:13:26,740
+و it causes conditions similar to cholera بسبب
+
+179
+00:13:26,740 --> 00:13:29,840
+الخط العزيزي هنا دياريا like cholera زي ما حكينا
+
+180
+00:13:29,840 --> 00:13:34,820
+milder أخف شوية من ال cholera لإن ال growth و ال
+
+181
+00:13:34,820 --> 00:13:38,060
+multiplication تبع الإجريشيا الcholera أقل درجة من
+
+182
+00:13:38,060 --> 00:13:42,280
+مين من ال vibrio cholera ممكن تتشر في الحضانات
+
+183
+00:13:42,280 --> 00:13:45,880
+يعني في الأطفال اللي عايشين في الحضانات أو أول
+
+184
+00:13:45,880 --> 00:13:48,000
+حاجة بيسموها travelers يعني ال travelers يعني يعني
+
+185
+00:13:48,000 --> 00:13:51,380
+المعروف عن إسهال المسافرينالذي ينتقل من مكان إلى
+
+186
+00:13:51,380 --> 00:13:56,020
+مكان وتتغذى عليه الطبيعة الجو والغذاء ممكن يصاب
+
+187
+00:13:56,020 --> 00:14:02,800
+بهذه الانفكار caused by LT ST أو both LT and ST LT
+
+188
+00:14:02,800 --> 00:14:06,760
+اللي هو ال bio-toxin وهو اللي يشبه الكودا توكسين
+
+189
+00:14:06,760 --> 00:14:13,120
+LT توكسين والST stable toxin يعني فكرة كلمة
+
+190
+00:14:13,120 --> 00:14:14,220
+الفايرو stable
+
+191
+00:14:17,150 --> 00:14:21,310
+إذا ال L-T و L-S-T توكسين اللي بيشتغله بالطريقة
+
+192
+00:14:21,310 --> 00:14:25,570
+التابعة ال heat-loving toxin like cholera gene او
+
+193
+00:14:25,570 --> 00:14:28,670
+cholera toxin it activates adenylate cyclase
+
+194
+00:14:28,670 --> 00:14:33,190
+enzyme بيرفع من ال cyclic AMP بيزيد من ال
+
+195
+00:14:33,190 --> 00:14:38,620
+secretions لل water ionsالـ hate stable toxin
+
+196
+00:14:38,620 --> 00:14:42,780
+بيشبه الأولان تماما في الميكانيزم بس بدل ما يشتغل
+
+197
+00:14:42,780 --> 00:14:46,620
+على قدرات Cyclase enzyme بيشتغل على جوانالات اللي
+
+198
+00:14:46,620 --> 00:14:49,800
+هو من الجوانين جوانالات Cyclase enzyme بيسيطروا
+
+199
+00:14:49,800 --> 00:14:55,040
+activation بيزيد كمية الـ cyclic GMP بدل ما يزيد
+
+200
+00:14:55,040 --> 00:14:58,260
+ال secretion ممنع ال absorption ماهي نفس المحصلة
+
+201
+00:14:58,260 --> 00:15:02,360
+ده إذا مانعنا الabsorption .. ال
+
+202
+00:15:04,570 --> 00:15:09,590
+أه فبيمنع ال .. ال .. ال ions من أنها تمتص و بتاع
+
+203
+00:15:09,590 --> 00:15:12,190
+اللي بيصير فيه كمية كبيرة من ال ions و الماء في
+
+204
+00:15:12,190 --> 00:15:18,850
+الداخل إذن ال heat level بيشبه لحد كبير ال heat
+
+205
+00:15:18,850 --> 00:15:22,550
+stable في النتيجة في المحاصلة لكن they work
+
+206
+00:15:22,550 --> 00:15:25,010
+different واحد بيشتغل على ال analyte واحد بيشتغل
+
+207
+00:15:25,010 --> 00:15:27,790
+على الgonalate هذا واحد واحد بيزيد ال secretion
+
+208
+00:15:27,790 --> 00:15:33,370
+واحد بيقلل من ال absorptionوهي واحدة اللى بيقدر او
+
+209
+00:15:33,370 --> 00:15:36,010
+بيزيد في الاخر بيصير فيه accumulation ل ال
+
+210
+00:15:36,010 --> 00:15:40,450
+electrolyte في الدسم هذه نفس المعلومات اللى بتوري
+
+211
+00:15:40,450 --> 00:15:43,310
+اللى هو ال toxin LT و ST كيف بيشتغلوا على اللى
+
+212
+00:15:43,310 --> 00:15:46,890
+اللى هو ال Y طبعا بتورينا كيف اشتركي بالله ال
+
+213
+00:15:46,890 --> 00:15:51,590
+toxin في امرأة microbe بتير مهم في الاشريشيا كلها
+
+214
+00:15:51,590 --> 00:15:53,250
+اسمه entero invasive
+
+215
+00:15:56,200 --> 00:16:00,200
+يعني حتى المصاميات هذه فيها على شوية خلافات، بمعنى
+
+216
+00:16:00,200 --> 00:16:03,480
+إنه قبل نجد إن واحد بيقول عنها intro-hemorrhagic،
+
+217
+00:16:03,480 --> 00:16:08,730
+وواحد بيقول عنها intro-invasiveفي مبرر لل .. لل ..
+
+218
+00:16:08,730 --> 00:16:14,650
+لل .. يعني لعدم الموضوع هذا ايش المبرر؟ انه بعض ال
+
+219
+00:16:14,650 --> 00:16:19,170
+strings بيمتلك صفتين او اكثر قد تشتركها مع بكتيري
+
+220
+00:16:19,170 --> 00:16:22,230
+اخرى و تنفصل عن بكتيري اخرى فبصير فيهم الاشتباه
+
+221
+00:16:22,230 --> 00:16:26,250
+فيها ده او خلينا نقول التباس في تسميع الميكروب لأ
+
+222
+00:16:26,250 --> 00:16:31,310
+احنا قدرنا نص اسمه بقول enteroinvasive Escherichia
+
+223
+00:16:31,310 --> 00:16:34,350
+coli هذا الميكروب عنده المفضل على انه يعمل
+
+224
+00:16:34,350 --> 00:16:35,930
+attachment لالميكروب
+
+225
+00:16:40,300 --> 00:16:43,600
+و البيولايت أقول عنها مكون أساسي في ال E.coli حتى
+
+226
+00:16:43,600 --> 00:16:47,160
+اللي بتسبب بيورنايترات infection لإن كيف بتعمل
+
+227
+00:16:47,160 --> 00:16:51,740
+بيوليفريتس عكس طيار صح؟ لازم يكون مباطلة هي على
+
+228
+00:16:51,740 --> 00:16:59,240
+الإلتصاق ال outer membranes بتبعت البكتيريا بتعمل
+
+229
+00:16:59,240 --> 00:17:05,300
+عملية modification في ال cell membrane تبع الخلالة
+
+230
+00:17:05,300 --> 00:17:08,920
+اللي هو ال cell و بتخلي البكتيريا تعملها
+
+231
+00:17:08,920 --> 00:17:13,150
+phagocytosisالان البكتيريا بالظبط البكتيريا ساعة
+
+232
+00:17:13,150 --> 00:17:17,410
+واحد موجودة الان جوا الخلايا ال intestinal cells
+
+233
+00:17:17,410 --> 00:17:22,950
+بتجي و تعمل destruction للخلايا عبر ال protein
+
+234
+00:17:22,950 --> 00:17:26,330
+synthesis inhibition فاكرين الشيجيلاتوكسين؟
+
+235
+00:17:26,330 --> 00:17:31,210
+الشيجيلاتوكسين عشان بيشتغل بالضبط عبر ال ribosome
+
+236
+00:17:31,210 --> 00:17:35,130
+و بوقف ال protein synthesisفال S لاحظوش ايش اخر
+
+237
+00:17:35,130 --> 00:17:39,010
+جبنة بتقولها هنا؟ it resembles .. يعني المعنى اللي
+
+238
+00:17:39,010 --> 00:17:42,310
+حاكي الأولانية اللي جابلها كان resemble كلها و هاد
+
+239
+00:17:42,310 --> 00:17:45,450
+resemble شجلة و هاد الكلاب اللي بقى اكد عليه ..
+
+240
+00:17:45,450 --> 00:17:51,150
+اللي اكد عليه المحاضرة السابقةانه في transfer of
+
+241
+00:17:51,150 --> 00:17:55,530
+information من genus ال Vibrio للإشريشية من genus
+
+242
+00:17:55,530 --> 00:18:00,650
+الشجلة للإشريشية وبتالي في gene transfer او على
+
+243
+00:18:00,650 --> 00:18:04,210
+الأقل هذا مؤشر ان في gene transfer بين genus و
+
+244
+00:18:04,210 --> 00:18:06,470
+genus طيب
+
+245
+00:18:06,470 --> 00:18:13,770
+البكيلوجوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهو
+
+246
+00:18:14,900 --> 00:18:17,260
+يعني مافيش داعي انها تتكاثر و تطلع بعض الخلية و
+
+247
+00:18:17,260 --> 00:18:21,100
+تطلع خلية تانية، لأ من خلية إلى خلية، بنسميها
+
+248
+00:18:21,100 --> 00:18:26,820
+horizontal تمام؟ او lateral symptoms include
+
+249
+00:18:26,820 --> 00:18:31,000
+fever، هنفسر ال symptoms من وين اجت في غالب
+
+250
+00:18:31,000 --> 00:18:33,760
+الأحيان، fever، severe abdominal cramps اللي هي
+
+251
+00:18:33,760 --> 00:18:34,460
+مغض شديد
+
+252
+00:18:47,670 --> 00:18:52,690
+هو عبارة عن ميوكاس and pus and blood، ممكن يكون
+
+253
+00:18:52,690 --> 00:18:57,970
+كمان إيه؟ blood فقط، لكن لإن دولاريا frequent،
+
+254
+00:18:57,970 --> 00:19:03,070
+مرارة كراية، مافيش حاجة، اسمها stool الآن، فقط
+
+255
+00:19:03,070 --> 00:19:06,970
+الناتج هو الميوكاس والخلايا الميتة والبكتيريا
+
+256
+00:19:06,970 --> 00:19:11,670
+والصديد اللي بينتج، هنعرف من كل واحد منهم من وين
+
+257
+00:19:11,670 --> 00:19:17,820
+إيهit resembles gigalosis بتاعنا من وين اجت هاي ال
+
+258
+00:19:17,820 --> 00:19:22,520
+.. ال intestinal wall هاي ال microvilli ال
+
+259
+00:19:22,520 --> 00:19:26,760
+microvilli عليها ال mucus هاي البكتيريا عشان تلتصق
+
+260
+00:19:26,760 --> 00:19:31,420
+لازم تزيح لمucus و تلتصق بمين؟ بالبكتيريا بال ..
+
+261
+00:19:31,420 --> 00:19:34,200
+بال microvilli الآن مش بحاجة لحاجة إن ال mucus
+
+262
+00:19:34,200 --> 00:19:38,800
+انزعح إنه راح لأ موجود لكن في مكان قريب الآن
+
+263
+00:19:38,800 --> 00:19:42,000
+البكتيريا دخلت جواو موضت في الخلية الأولى و
+
+264
+00:19:42,000 --> 00:19:44,260
+التانية و التالتة و الرابعة و الخمسة أعلى تسطر من
+
+265
+00:19:44,260 --> 00:19:47,480
+الخلايا اللي موضتهم الخلايا بيختفوش عن وجه الأرض
+
+266
+00:19:47,480 --> 00:19:53,400
+فجأة لكن بيقولهم ميتين الاسم بيتخلص منهم و بيصيلهم
+
+267
+00:19:53,400 --> 00:19:57,240
+sloughing sloughing يعني ايه؟ يعني بيتقشر في
+
+268
+00:19:57,240 --> 00:20:01,040
+المعمل الآن بينزلوا و منزلوا في ال intestinal
+
+269
+00:20:01,040 --> 00:20:04,480
+lumen في اللي من في التجريب ايش عليهم الخلايا
+
+270
+00:20:04,480 --> 00:20:07,900
+دولة؟ هم lipidical cells ميتة فيهم بكتيريا و عليهم
+
+271
+00:20:07,900 --> 00:20:13,330
+ميوكاسيبقى الميوكاس اللى نزل فى الستول نادى عن
+
+272
+00:20:13,330 --> 00:20:17,630
+مين؟ عن موت الخلايا و سقوطها، يبقى بتوقع علاج فى
+
+273
+00:20:17,630 --> 00:20:21,390
+الستول ميوكاس ذات Epithelial cells ذات البكتيريا
+
+274
+00:20:21,390 --> 00:20:24,950
+نفسها ليها جرام نيجاتيف بصلاي أو باندنطلي، يعني
+
+275
+00:20:24,950 --> 00:20:25,970
+بشكل كبير
+
+276
+00:20:34,860 --> 00:20:37,400
+هى اللى بتاخد ال nutrient اللى ساعد على ال
+
+277
+00:20:37,400 --> 00:20:41,680
+absorption صح من ال micro villi بتاخدها و بتوزعها
+
+278
+00:20:41,680 --> 00:20:45,280
+على الرسم يبجى على طول ورا ال villi هيكون فيه
+
+279
+00:20:45,280 --> 00:20:49,380
+capillaries فهذه مصنوعة من PVC ولا plastic ولا ايش
+
+280
+00:20:49,380 --> 00:20:55,260
+مصنوعة مش برضه خلايا مش epithelial cells البكتيريا
+
+281
+00:20:55,260 --> 00:20:58,860
+بتتسخ فيها و بتعملها destruction يبجى ال blood
+
+282
+00:20:58,860 --> 00:21:01,700
+هيسير او ال capillaries هيسير لها rupture يعني
+
+283
+00:21:01,700 --> 00:21:06,930
+هتنفجر يبجى هينزلدم في الستولة يعني في عند ال
+
+284
+00:21:06,930 --> 00:21:10,870
+blood و في عند ال mucus طب إيش اللي جاب الصديد؟
+
+285
+00:21:10,870 --> 00:21:16,630
+كلكوا بتعرفوا إن خليانة عندما تموت تصرخ تصرخ لتنبه
+
+286
+00:21:16,630 --> 00:21:19,470
+جهاز المنع الصراخ تبعها عبارة عن cytokines
+
+287
+00:21:19,470 --> 00:21:24,210
+messengers طبعا؟ ابتدي حضرة ال .. ال .. ال .. ال
+
+288
+00:21:24,210 --> 00:21:24,730
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+289
+00:21:24,730 --> 00:21:25,210
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+290
+00:21:25,210 --> 00:21:25,290
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+291
+00:21:25,290 --> 00:21:26,870
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+292
+00:21:26,870 --> 00:21:27,130
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+293
+00:21:27,130 --> 00:21:27,950
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+294
+00:21:27,950 --> 00:21:28,490
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+295
+00:21:28,490 --> 00:21:32,500
+ال .. ال ..الشجلة معروفة عنها خدوا بالكوا انها can
+
+296
+00:21:32,500 --> 00:21:36,740
+exist intracellular مش سهل نقتلها بمين؟ بال
+
+297
+00:21:36,740 --> 00:21:41,600
+phagocytes فكان مينية كبيرة من الخلايا اللي جاية
+
+298
+00:21:41,600 --> 00:21:48,420
+تحارب بتموت من مين؟ من الشجلة وبالتالي موت الخلايا
+
+299
+00:21:48,420 --> 00:21:52,660
+اللي هي ال phagocytes بينتج عنه صديد الصديد هو ال
+
+300
+00:21:52,660 --> 00:21:52,960
+bus
+
+301
+00:21:56,430 --> 00:22:00,170
+بتا هي عبارة عن ايه؟ عن white blood cell ماتة اللي
+
+302
+00:22:00,170 --> 00:22:05,550
+هي ال neutral field إذا انفسرنا نزول ال bus، نزول
+
+303
+00:22:05,550 --> 00:22:09,770
+الدم، نزول ال mucus، نزول ال epithelial cells،
+
+304
+00:22:09,770 --> 00:22:12,990
+نزول كمية كبيرة من اللي جراها، negative بس الله،
+
+305
+00:22:12,990 --> 00:22:16,730
+بالإضافة إلى الديارة، ما فسرناها خلاص الديارة،
+
+306
+00:22:16,730 --> 00:22:20,150
+مافيش epithelial cells تنفس، accumulation ديارة
+
+307
+00:22:21,290 --> 00:22:24,450
+بصرة الصراحية يبقى معنا بحكي أنه هذه البكتيريا
+
+308
+00:22:24,450 --> 00:22:28,710
+بتعمل شجلة like يبقى بنقدر نقول عنها dysentery
+
+309
+00:22:28,710 --> 00:22:33,570
+كلمة dysentery هي مميزاتها أنه فيها عندى stool،
+
+310
+00:22:33,570 --> 00:22:39,410
+دياريا، دياريا فيها blood، فيها mucus وفيها blood
+
+311
+00:22:39,410 --> 00:22:44,650
+و mucus بص و بالظبط بص و طبعا بالله فانا انا اقول
+
+312
+00:22:44,650 --> 00:22:50,760
+ان انا epithelial sexDysentery,Dysentery,Dysentery
+
+313
+00:22:50,760 --> 00:22:54,840
+,Dysentery,Dysentery,Dysentery,Dysentery,Dysentery
+
+314
+00:22:54,840 --> 00:22:54,840
+,Dysentery,Dysentery,Dysentery,Dysentery,Dysentery
+
+315
+00:22:54,840 --> 00:22:56,600
+,Dysentery,Dysentery,Dysentery,Dysentery,Dysentery
+
+316
+00:22:56,600 --> 00:22:57,000
+,Dysentery,Dysentery,Dysentery,Dysentery,Dysentery
+
+317
+00:22:57,000 --> 00:22:57,060
+,Dysentery,Dysentery,Dysentery,Dysentery,Dysentery
+
+318
+00:22:57,060 --> 00:23:06,480
+,Dysentery
+
+319
+00:23:06,480 --> 00:23:06,480
+,Dysentery,Dysentery,Dysentery,Dysentery,Dysentery
+
+320
+00:23:06,480 --> 00:23:06,560
+,Dysentery,Dysentery,Dysentery,Dysentery,Dysentery
+
+321
+00:23:06,560 --> 00:23:10,560
+,Dysentery,Dysentery,Dysentery,Dysentery,Dysentery
+
+322
+00:23:10,560 --> 00:23:14,220
+,Dysentery
+
+323
+00:23:15,170 --> 00:23:18,330
+فى برايل يعني مافيش حرارة، مافيش سخونة عند المرض
+
+324
+00:23:18,330 --> 00:23:23,270
+يكون أحد أهم أخطر ال conditions اللى بتسببها هذه
+
+325
+00:23:23,270 --> 00:23:27,690
+البكتيريا هي ما يسمى بال HUS Hemolytic Uremic
+
+326
+00:23:27,690 --> 00:23:30,510
+Syndrome
+
+327
+00:23:32,270 --> 00:23:35,210
+وانا فجأة فانده مطورة لإنه انا باعتقادي ان هو
+
+328
+00:23:35,210 --> 00:23:38,550
+الميكروب يعني نوع ما مهمل في قطر غزة على الرغم من
+
+329
+00:23:38,550 --> 00:23:43,450
+انه الدراسات اللي أجريت محليا أثبتت وجود الميكروب
+
+330
+00:23:43,450 --> 00:23:47,690
+في مرضى و أثبتت وجود الميكروب في عينات غذائية منها
+
+331
+00:23:47,690 --> 00:23:53,000
+اللحم، الحوم، منها الدواجن، منها الخضرواتاللي تأكل
+
+332
+00:23:53,000 --> 00:23:57,960
+طازجة بمعناه انه ماتتعرضش لحرارة عالية فالبكتيريا
+
+333
+00:23:57,960 --> 00:24:02,700
+موجودة لكن الكشف عنها للأسف يعني عليها الاستفادة
+
+334
+00:24:02,700 --> 00:24:08,560
+مستشفياتنا ماتتجمش بالواقع في هذا الإطار لذلك هناك
+
+335
+00:24:08,560 --> 00:24:16,660
+بعض الأطفال قد نفقدهم بسبب فشل كله ناتج عن الإصابة
+
+336
+00:24:16,660 --> 00:24:23,130
+بهذا الميكروب بيُعطى مريض أنتبايتكفجأة اللي ما يخف
+
+337
+00:24:23,130 --> 00:24:28,330
+بتزيد الحالة عنده، ليش؟ لأن الخلايا لما تموت بتزيد
+
+338
+00:24:28,330 --> 00:24:31,930
+ال destruction اللي موجود طب احنا مش عارفين السبب
+
+339
+00:24:31,930 --> 00:24:34,610
+و قبلتها اللي بيجرب و انا انت بيتك واحد و اتنين و
+
+340
+00:24:34,610 --> 00:24:38,350
+تلاتة و اربع و للأسف ممكن يموت زي ما حكينا من
+
+341
+00:24:38,350 --> 00:24:43,410
+kidney failure و طفل kidney failure ناتجة عن
+
+342
+00:24:43,410 --> 00:24:46,270
+الاشريشيا كولا اللي سمينها in terror يمرجك اشريشيا
+
+343
+00:24:46,270 --> 00:24:51,090
+كولابتعرفوا الطعام التمييز بينها بتعمل hemolytic
+
+344
+00:24:51,090 --> 00:24:53,650
+anemia وبتعمل thrombocytopenia اللي اللي هو
+
+345
+00:24:53,650 --> 00:25:02,670
+platelet camp تسمعوا بالاسم هدا O157H7
+
+346
+00:25:02,670 --> 00:25:07,570
+هي ال endo hemolytic E.coli هي نفسها ال E.coli
+
+347
+00:25:07,570 --> 00:25:12,910
+O157H7 اللي سمى في بريطانيا وامريكا hamburger
+
+348
+00:25:12,910 --> 00:25:14,510
+basselie او burger basselie
+
+349
+00:25:17,420 --> 00:25:22,940
+أول حالات تم اكتشافها للإيصال على الميكروب روبطت ب
+
+350
+00:25:22,940 --> 00:25:28,600
+under cooked hamburger under cooked يعني مش مطهي
+
+351
+00:25:28,600 --> 00:25:32,080
+جيدا و انا بقولكوا شغل في موضوع الطهي جيدا و مش
+
+352
+00:25:32,080 --> 00:25:37,820
+جيدا لأن عندهم هناك طبعا احنا .. اش .. اش العادة
+
+353
+00:25:37,820 --> 00:25:40,720
+الغذائية عندنا لما بنطلع رحلة مثلا بناخد نشوي على
+
+354
+00:25:40,720 --> 00:25:45,530
+البحر او في الخلاء مثلا او في منطقة و لا لا نشويهم
+
+355
+00:25:45,530 --> 00:25:48,770
+اكتر اشي بيعملوا بيعملوا اقراص همبرجر برضه
+
+356
+00:25:48,770 --> 00:25:54,050
+بيشوفوها فالان و جهزولهم ترمومترات قص الهمبرجر و
+
+357
+00:25:54,050 --> 00:25:57,550
+نحطوا فيه ترمومتر من جوا عشان نتأكل اذا اتحارست 75
+
+358
+00:25:57,550 --> 00:26:01,870
+درجة او ايه عشان خاطر .. عشان خاطر نطمئن ان هذه
+
+359
+00:26:01,870 --> 00:26:07,330
+البكتيرا تكون ايه ماتة فبعند ال .. ال .. ال .. ال
+
+360
+00:26:07,330 --> 00:26:13,120
+ground beef او ال ground meat اللحم المفرومبيحتاجش
+
+361
+00:26:13,120 --> 00:26:16,180
+لحرارة عالية عشان ينتهي وانتوا بتعرفوا الفرق بين
+
+362
+00:26:16,180 --> 00:26:19,580
+انتوا معارف ال .. في الأمور هذه وابتعرفوا الفرق
+
+363
+00:26:19,580 --> 00:26:23,600
+بين طغي لحم مفروم وغير مفروم تمام؟ هذا ليه استغرف
+
+364
+00:26:23,600 --> 00:26:27,180
+وقت؟ وانا مستغرف وقت فبتعرف مادام مابستغرقش وقت دي
+
+365
+00:26:27,180 --> 00:26:32,200
+بالحرارة مابتصلش للمنطصف ب .. ب .. ب .. ب .. بدرجة
+
+366
+00:26:32,200 --> 00:26:35,020
+كتلة انها تكون قتلة ايه؟ الميكروباطام وبعدين بنقول
+
+367
+00:26:35,020 --> 00:26:39,240
+undercooked undercooked meat ممكن يكون سبب الأسباب
+
+368
+00:26:39,240 --> 00:26:45,560
+انتقال هذا ال micro orgasmفي كلمة شركة تانية اسمها
+
+369
+00:26:45,560 --> 00:26:52,120
+virotoxin و برضه هم عطيلة اسمها شركة like لإن عشان
+
+370
+00:26:52,120 --> 00:26:55,820
+انا اقولكوا في خلط ما بين ال entero invasive و ال
+
+371
+00:26:55,820 --> 00:27:00,210
+entero hemorrhageإشان الفيروتوكسين هنبدا أشرح و
+
+372
+00:27:00,210 --> 00:27:03,270
+هننهي في محالتنا عشان أعطيكوا فرصة كويسة أنه تلتقط
+
+373
+00:27:03,270 --> 00:27:06,710
+الأنفاس للاختبار ان شاء الله اليوم الفيروتوكسين
+
+374
+00:27:06,710 --> 00:27:10,810
+سمى الفيروتوكسين لإنه له تأثير على فيروس ال
+
+375
+00:27:10,810 --> 00:27:14,310
+culture إشان الفيروس ال culture نرجع لأفريقيا و
+
+376
+00:27:14,310 --> 00:27:18,970
+نروح على الغابات و نطلع على الشجرة و نلاقي قرد
+
+377
+00:27:18,970 --> 00:27:24,870
+أخضر اسمه African green monkey kidney أفضل African
+
+378
+00:27:24,870 --> 00:27:28,910
+green monkeyالـ African Green Monkey هدولة أخدنا
+
+379
+00:27:28,910 --> 00:27:32,230
+منهم kidney cells يعني أخدنا ال kidney تبعتهم و
+
+380
+00:27:32,230 --> 00:27:35,890
+أخدنا منها خلايا و عملنا منها cell culture cell
+
+381
+00:27:35,890 --> 00:27:41,190
+line cell line اسمه Vero cell line يبقى ال Vero من
+
+382
+00:27:41,190 --> 00:27:45,370
+عندي هي من اسم ال cell culture اللي أخدناها من ال
+
+383
+00:27:45,370 --> 00:27:49,370
+African Green Monkey تمام؟ أخدناها من عندي؟ من ال
+
+384
+00:27:49,370 --> 00:27:54,050
+kidneys يبقى أنا اللي عندي Vero cell cultureخونوا
+
+385
+00:27:54,050 --> 00:27:56,690
+بالكوا ان انتوا الانطباع اللى بتعرفوه على ال cell
+
+386
+00:27:56,690 --> 00:28:01,210
+culture انه بنستخدمها لدل مات مين؟ للمكتيريا؟ لأ
+
+387
+00:28:01,210 --> 00:28:06,330
+cell culture، للفيروسات، ال cell culture بننامي
+
+388
+00:28:06,330 --> 00:28:08,870
+فيها الفيروسات، احنا بننامي البكتيريا على
+
+389
+00:28:08,870 --> 00:28:11,810
+المقومكي، انا بسميها artificial media، مظبوط هذه
+
+390
+00:28:11,810 --> 00:28:13,890
+cell culture، هذه لمين؟ للفيروسات
+
+391
+00:28:17,450 --> 00:28:22,150
+من ضمن هذه الأغراض انه اشوف موارد سامة تأثير موارد
+
+392
+00:28:22,150 --> 00:28:26,150
+سامة على الخلايا طبعا الخلايا لما احنا بناخد من
+
+393
+00:28:26,150 --> 00:28:32,130
+كدني و بنزرعها في المعمل تطلعش كدني علما تطلع ما
+
+394
+00:28:32,130 --> 00:28:36,650
+يسمى ب mono layer فبقعة من الخلايا على السطح اللي
+
+395
+00:28:36,650 --> 00:28:41,150
+احنا زرعناها عليها mono layer فبقعة واحدة تحت
+
+396
+00:28:41,150 --> 00:28:46,060
+المايكروسكوب نقدر نشوف الخلايا بشكل واضحوكل نوع من
+
+397
+00:28:46,060 --> 00:28:51,640
+الخلايا إله شكله المميز تبعه وكل فيروس أو كل مركب
+
+398
+00:28:51,640 --> 00:28:54,820
+بيعمل destruction برضه ممكن يكون ال destruction
+
+399
+00:28:54,820 --> 00:29:01,760
+ميزة تميز الفيروس بمعنى بعض الفيروسات بتعمل
+
+400
+00:29:01,760 --> 00:29:07,320
+license للخلايا بتدمرهابعض الفيروسات بتجمع الخلايا
+
+401
+00:29:07,320 --> 00:29:10,680
+أربعة أو خمسة في خلية واحدة، بيسموها syncytia،
+
+402
+00:29:10,680 --> 00:29:14,380
+طبعا؟ فكل فيروس أو كل مُركّب له حاجة بيسموها
+
+403
+00:29:14,380 --> 00:29:19,020
+cytopathic effect، تأثيرات باطولوجية على الخلايا
+
+404
+00:29:19,860 --> 00:29:23,640
+لما جيبنا ال toxin تال إيشيشيكولاي اللي هي ال
+
+405
+00:29:23,640 --> 00:29:27,300
+enterohemorrhagic حطناها على الفيروس العمال
+
+406
+00:29:27,300 --> 00:29:31,880
+destruction، فما يسميناه فيرو توكسين، يعني سام
+
+407
+00:29:31,880 --> 00:29:35,340
+لغلايا الفيرو، عرفنا ايه الفيرو؟ عرفنا ايه؟ على
+
+408
+00:29:35,340 --> 00:29:42,770
+اخره، بتنتج ايضا hemolysinsغالبا بتصيب children
+
+409
+00:29:42,770 --> 00:29:46,430
+younger than 5 years old وبتسبب Hemorrhagic
+
+410
+00:29:46,430 --> 00:29:49,990
+Colitis Hemorrhagic Colitis يعني التهاب في القضاء
+
+411
+00:29:49,990 --> 00:29:54,490
+ونزف يكون فيه نزف نكمل ان شاء الله المرة القادمة
+
+412
+00:29:54,490 --> 00:29:56,810
+بعد اللي هو الامتحان
+
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/m85QUrbJGqM.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/m85QUrbJGqM.srt
new file mode 100644
index 0000000000000000000000000000000000000000..835fd420362283eac55e206ef09437cbbcc879d3
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/m85QUrbJGqM.srt
@@ -0,0 +1,2667 @@
+1
+00:00:20,870 --> 00:00:23,330
+بسم الله الرحمن الرحيم الحمد لله رب العالمين عليه الصلاة
+
+2
+00:00:23,330 --> 00:00:26,330
+والسلام وعلى سيدنا محمد وعلى آله وصحبه أجمعين 
+
+3
+00:00:26,330 --> 00:00:30,330
+وبداية هذا الفصل الدراسي نرحب بكم 
+
+4
+00:00:30,330 --> 00:00:36,190
+في مساق الأحياء الدقيقة الطبية الأساسية أو كما 
+
+5
+00:00:36,190 --> 00:00:38,550
+تدعى باللغة الإنجليزية essential medical microbiology
+
+6
+00:00:38,550 --> 00:00:43,530
+مش هندرس اليوم microbiology لكن هنحاول قدر
+
+7
+00:00:43,530 --> 00:00:47,850
+الإمكان نسوق لهذا المساق نقول لكم أن هو هذا المساق
+
+8
+00:00:47,850 --> 00:00:53,120
+رائع جيد ممتاز أساسي وضروري لمهنة العلم الطبية
+
+9
+00:00:53,120 --> 00:00:56,760
+المخبرية يعني مش من المساقات الثانوية وإنما من
+
+10
+00:00:56,760 --> 00:01:01,700
+المساقات الأساسية حتى نبدأ بالتسويق لهذا المساق
+
+11
+00:01:01,700 --> 00:01:04,540
+بدنا نبدأ من الكلمة الأخيرة microbiology
+
+12
+00:01:04,540 --> 00:01:07,640
+microbiology كلكم تعرفون التعريف تبعها أنه هي
+
+13
+00:01:07,640 --> 00:01:11,360
+دراسة الأحياء فكرة عامة micro بتكلم عن دراسة
+
+14
+00:01:11,360 --> 00:01:15,140
+الأحياء الدقيقة يعني لو بدنا نعرف تعريف بسيط بنقول
+
+15
+00:01:15,140 --> 00:01:19,380
+the study of microorganism  الـ key word في هذا
+
+16
+00:01:19,380 --> 00:01:23,040
+الموضوع كلمة micro organism what is a micro
+
+17
+00:01:23,040 --> 00:01:27,720
+organism micro organism is a small living micro
+
+18
+00:01:27,720 --> 00:01:30,920
+organism that cannot be seen by the naked أو اللي
+
+19
+00:01:30,920 --> 00:01:35,740
+بيسميها unaided eye يعني العين المجردة أو العين
+
+20
+00:01:35,740 --> 00:01:39,520
+اللي مالهاش مساعدة مالهاش نظارات مالهاش microscope
+
+21
+00:01:39,520 --> 00:01:45,660
+لها إذا هذا هو الـ micro biome لكن حتى التعريف هذا
+
+22
+00:01:45,660 --> 00:01:49,550
+اللي احنا عرفناه للأسف نضطر نعمل justification أو
+
+23
+00:01:49,550 --> 00:01:56,610
+تبريرات لماذا ضمنا دودة طولها 7 متر ضمن الـ micro
+
+24
+00:01:56,610 --> 00:02:00,130
+بيولوجيا في علم البراسيتولوجيا ولماذا دودة
+
+25
+00:02:00,130 --> 00:02:04,030
+الأسكاريس اللي طولها 30 سنتي برضه مدرسة تحت
+
+26
+00:02:04,030 --> 00:02:06,890
+الـ مايكرو هل .. هل بدنا مايكروسكوب حتى نشوف دودة 30
+
+27
+00:02:06,890 --> 00:02:13,010
+سنتي مصطرة؟ بدناش مايكروسكوب one stage of the life
+
+28
+00:02:13,010 --> 00:02:16,190
+cycle of this parasite is microscopic يعني مرحلة
+
+29
+00:02:16,190 --> 00:02:23,010
+مرحلة حياتها بتكون مجهرية برضه مش مبرر احنا كبشر
+
+30
+00:02:23,010 --> 00:02:27,490
+مرحلة من مراحل حياتنا بنكون مجهرين برضه صح ولا لأ
+
+31
+00:02:27,490 --> 00:02:34,630
+التبرير الثاني أن they cause disease يعني بسببه
+
+32
+00:02:34,630 --> 00:02:37,850
+أمراض زي البكتيريا اللي هي بالفعل بينطبق عليها
+
+33
+00:02:37,850 --> 00:02:42,310
+التعريف بنعمل كمان justification الفيروسات نحن كنا
+
+34
+00:02:42,310 --> 00:02:46,990
+نتكلم عن living microorganisms هل الفيروسات living
+
+35
+00:02:46,990 --> 00:02:53,250
+microorganisms؟ هل هي أصلا ينطبق عليها organism؟
+
+36
+00:02:53,250 --> 00:02:57,370
+هل هي خلوية؟ يعني إيه الخلية؟ الفيروسات؟ مالهاش
+
+37
+00:02:57,370 --> 00:03:01,950
+وبالتالي ينطبق عليها جزء الأول من التعريف اللي هو
+
+38
+00:03:01,950 --> 00:03:06,470
+كلمة micro لأنها small لكن لم ينطبق عليها biology
+
+39
+00:03:06,910 --> 00:03:09,730
+وما نروح نجرّسها في الـ microbiology justification
+
+40
+00:03:09,730 --> 00:03:17,230
+تبرير أن الفيروسات تمتلك صفة من صفات الخلايا الحية
+
+41
+00:03:17,230 --> 00:03:23,430
+اللي هي التكاثر عن طريق أنها تستخدم host cell أو
+
+42
+00:03:23,430 --> 00:03:28,350
+يعني خلايا عائل معين نبات أو حيوان أو حتى بعض
+
+43
+00:03:28,350 --> 00:03:32,110
+الفيروسات تتطفل على الـ bacteria بيسموها
+
+44
+00:03:32,110 --> 00:03:35,550
+bacteriophytes الفطريات
+
+45
+00:03:36,710 --> 00:03:41,410
+كلكم أكلتم مشروم المشروم فطرية البعض منكم أصيبوا
+
+46
+00:03:41,410 --> 00:03:45,190
+بين أصابع أرجله التهابات فطرية اللي بتطلع رائحة
+
+47
+00:03:45,190 --> 00:03:49,470
+كريهة بعض الأحيان عند لبس الأحذية لفترات طويلة اللي
+
+48
+00:03:49,470 --> 00:03:54,290
+بيسموها قدم رياضية قدم رياضية ويعني بمعنى آخر في
+
+49
+00:03:54,290 --> 00:03:58,910
+microscopic وفي macroscopic لكن الـ sports تبعتها
+
+50
+00:03:58,910 --> 00:04:03,130
+كلها microscopic سواء كانت الفطريات الكبيرة أو
+
+51
+00:04:03,130 --> 00:04:07,090
+الفطريات الـ microscopic الـ sports تبعتهاmicroscope
+
+52
+00:04:07,090 --> 00:04:11,630
+إذا متفقين على الـ microbiology على تعريفها وعلى
+
+53
+00:04:11,630 --> 00:04:15,710
+التبريرات لماذا ضمنها بعض المخلوقات اللي هي لا
+
+54
+00:04:15,710 --> 00:04:20,370
+ينطبق عليها التعريف ضمن هذا التعريف نيجي لكلمة
+
+55
+00:04:20,370 --> 00:04:25,250
+medical ليش خصصنا medical شوفوا أنا في الـ
+
+56
+00:04:25,250 --> 00:04:29,310
+microbiology أنا أعمل في مجال الـ food microbiology
+
+57
+00:04:29,310 --> 00:04:35,130
+كلمة food food testing services يعني في عملية فحص
+
+58
+00:04:35,130 --> 00:04:38,570
+الأغذية، فحص سلامة الأغذية في زمانا إيه اللي
+
+59
+00:04:38,570 --> 00:04:43,110
+بيشتغلوا في مجال تصنيع الأغذية، microbiology إيه
+
+60
+00:04:43,110 --> 00:04:45,970
+في مجال الـ environmental microbiology، البيئة،
+
+61
+00:04:45,970 --> 00:04:48,470
+بروح على البحر باخد عينات وبعملها testing، هذا
+
+62
+00:04:48,470 --> 00:04:53,230
+بيئة ولا لأ، برضه ناخد من الهواء عينات و بنتحصها،
+
+63
+00:04:53,230 --> 00:04:57,430
+هذا environmental microbiology قبل قدر من السنوات
+
+64
+00:04:57,430 --> 00:05:01,670
+أنتجنا بكتيريا لمكافحة البعوض، كنا .. يعني نمّي
+
+65
+00:05:01,670 --> 00:05:05,160
+البكتيريا، بدنا البكتيريا نفسها وبنرشها على
+
+66
+00:05:05,160 --> 00:05:10,660
+المستنقعات يرقات البعوض بتاكلها وبتموت هذا اسمه
+
+67
+00:05:10,660 --> 00:05:14,500
+Environmental و Industrial في نفس الـ logic ليش؟ احنا
+
+68
+00:05:14,500 --> 00:05:18,380
+قضينا على بعوض أو مش قضينا بالضبط خففنا لأن كلمة
+
+69
+00:05:18,380 --> 00:05:22,720
+قضينا موضوع صعب خففنا من البعوض بهذه التقنية إذا
+
+70
+00:05:22,720 --> 00:05:26,240
+الـ microbiology في مجالات متنوعة حمض الليمون اللي
+
+71
+00:05:26,240 --> 00:05:29,940
+بيستخدموه في المطبخ هذا 99.9% من
+
+72
+00:05:29,940 --> 00:05:38,130
+10 في الأسواق هو منتوج من الفطريات معظم
+
+73
+00:05:38,130 --> 00:05:42,290
+الـ antibiotics اللي بتتناولوها في العلاجات هي منتج
+
+74
+00:05:42,290 --> 00:05:47,270
+من منتجات الـ micro organism يبقى ما أقدرش أحصل الـ
+
+75
+00:05:47,270 --> 00:05:51,470
+microbiology في الـ medical microbiology ولا أقدر
+
+76
+00:05:51,470 --> 00:05:55,770
+أسيب الـ microbiology فاضية مفتوحة فاضية فاضية مظبوط
+
+77
+00:05:55,770 --> 00:05:59,510
+وبالتالي حددت أنا في الـ industrial أقول لكم في الـ food
+
+78
+00:05:59,510 --> 00:06:02,960
+microbiology صح في أن water microbiology مع أن الـ
+
+79
+00:06:02,960 --> 00:06:06,520
+water كان من ضمن الـ food في الـ food microbiology
+
+80
+00:06:06,520 --> 00:06:10,440
+صرنا نصنف الأغذية ونعطيها كاسم microbiology دلوقت
+
+81
+00:06:10,440 --> 00:06:15,780
+على توسع العلوم أو علم الميكروبيولوجي وتداخله في كل
+
+82
+00:06:15,780 --> 00:06:20,220
+المجالات هأعطيكم مجال رائع جدا قبل سنتين دخلنا
+
+83
+00:06:20,220 --> 00:06:25,360
+تعرفون الخرسانة؟ لمين بيشتغلون بالخرسانة في الجامعة؟
+
+84
+00:06:25,360 --> 00:06:28,360
+ل هندسة المدنية دلوقت هم اللي بيشتغلوا بالخرسانة احنا
+
+85
+00:06:28,360 --> 00:06:33,170
+مش دخلنا فيه بالخرسانة طيارة من قسم التخصصات الطبية
+
+86
+00:06:33,170 --> 00:06:38,270
+لأن هي مؤهلة وبتدرس ماجستير في الخرسانة عملنا
+
+87
+00:06:38,270 --> 00:06:43,690
+دراسة على استخدام البكتيريا في تقوية الإسمنت
+
+88
+00:06:43,690 --> 00:06:47,170
+المسلح يعني أضفنا بكتيريا من نوع معين وأضفنا
+
+89
+00:06:47,170 --> 00:06:52,330
+بعض الـ chemicals نعمل reaction نخلي الخرسانة يزداد
+
+90
+00:06:52,330 --> 00:06:55,510
+قدرته أو بيسموه compressive strength test قوة
+
+91
+00:06:55,510 --> 00:07:03,930
+تحمله إلى 30% إضافي وقللنا من قدرة امتصاصه إلى أكثر
+
+92
+00:07:03,930 --> 00:07:09,890
+من 30% أيضا بمعنى آخر صار أن الإسمنت مقاوم للرطوبة و
+
+93
+00:07:09,890 --> 00:07:14,670
+إسمنت أكثر صلابة وأكثر قدرة على التحمل كل هذا ضمن
+
+94
+00:07:14,670 --> 00:07:22,570
+من؟ لكن للأسف جدش الصورة اللي صورتها جميلة السيئين
+
+95
+00:07:23,220 --> 00:07:27,920
+من الميكروبات هم اللي أكثر شهرة يعني هم الأكثر
+
+96
+00:07:27,920 --> 00:07:32,340
+شيوعا في العالم وبالتالي لما بنقول ميكروبات بيخطر
+
+97
+00:07:32,340 --> 00:07:36,220
+على بالك ليش جيد و ليش سيء سيء لأن الـ medical
+
+98
+00:07:36,220 --> 00:07:42,560
+microbi الإنسان يتذكر فقط من الميكروبات اللي سبّبت له
+
+99
+00:07:42,560 --> 00:07:47,180
+أضرار لكن تلك اللي تسبب أضرار لا تشكل 1 إلى 2
+
+100
+00:07:47,180 --> 00:07:52,900
+في المئة من مجموعة الميكروبات إذا من 98% إلى 99% من
+
+101
+00:07:52,900 --> 00:07:58,480
+الميكروبات ذو فائدة وذو فائدة عظيمة تخيلوا ..
+
+102
+00:07:58,480 --> 00:08:05,420
+تخيلوا لو أن أشعة ما ضربت كوكب الأرض وقتلت كل
+
+103
+00:08:05,420 --> 00:08:09,220
+البكتيريا والمخلوقات الميكرو أورجينزية ما الذي
+
+104
+00:08:09,220 --> 00:08:12,920
+سيحدث .. عدا أني هأبطل أدرس micro biology أنا بقى
+
+105
+00:08:12,920 --> 00:08:16,660
+مدمر الميكروبات خلاص ما الذي سيحصل لكوكب الأرض
+
+106
+00:08:16,660 --> 00:08:24,420
+بعد عدة سنوات كل المخلوقات هتموت إذا بنقدر نقول إنه
+
+107
+00:08:24,420 --> 00:08:28,060
+هدف احنا بنحن نتكلم عن ناحية نظرية ليش استنتجنا
+
+108
+00:08:28,060 --> 00:08:33,840
+هذا الاستنتاج؟ لأنه الطاقة الكامنة فينا احنا ..
+
+109
+00:08:33,840 --> 00:08:39,020
+احنا كانت طاقة كامنة خزّنّاها بشكل غذاء تخزّن بشكل
+
+110
+00:08:39,020 --> 00:08:42,260
+carbohydrate و protein ودهون في جسم الإنسان
+
+111
+00:08:42,260 --> 00:08:45,920
+بالإضافة إلى عناصر زي الـ carbon و الـ nitrogen و الـ
+
+112
+00:08:45,920 --> 00:08:48,500
+phosphorus و الـ oxygen كل هذه العناصر اللي مختزنة
+
+113
+00:08:48,500 --> 00:08:53,180
+في أجسامنا تخيلوا أن هذه العناصر لم تدور في
+
+114
+00:08:53,180 --> 00:08:56,560
+الطبيعة لم تدور يعني ما أنا آخر ما نموتناش احنا
+
+115
+00:08:56,560 --> 00:08:59,460
+ما تحللناش مش أقصد احنا بس احنا على مستوانا
+
+116
+00:08:59,460 --> 00:09:03,420
+المخلوقات الأخرى جميعا النباتات طب مين اللي بيقوم
+
+117
+00:09:03,420 --> 00:09:07,320
+بعمل التحلل هذه الـ micro organisms إذا الـ micro
+
+118
+00:09:07,320 --> 00:09:12,320
+organisms بتقوم بدور رائع جدا في الطبيعة وبالتالي
+
+119
+00:09:12,320 --> 00:09:16,560
+لا يجب علينا أن ننسى كل هذا الدور الرائع واللخص
+
+120
+00:09:16,560 --> 00:09:20,360
+احنا في مين في الـ medical microbial ننتقل أقل مين
+
+121
+00:09:20,360 --> 00:09:25,560
+licensureessential يعني بيسموها أساسي لأ مش أساسي
+
+122
+00:09:25,560 --> 00:09:30,560
+ضروري فهل يعني .. هل معناها أن هن .. هن .. هنفصل
+
+123
+00:09:30,560 --> 00:09:34,460
+كثيرا في الـ .. في المصادر هذا ولا هناخد المعلومات
+
+124
+00:09:34,460 --> 00:09:37,480
+الأساسية غالبا هناخد المعلومات الأساسية الطبية
+
+125
+00:09:37,480 --> 00:09:42,740
+بتوسع وليس بعمق بمعنى هناخد معلومات كبيرة كثيرة 
+
+126
+00:09:42,740 --> 00:09:49,640
+عفواً لكن مش عمق أنا حاولت أقول لكم أن ال
+
+127
+00:09:49,640 --> 00:09:54,750
+microbiology  مرتبطة كثيراً بتعريف كلمة 
+
+128
+00:09:54,750 --> 00:09:58,090
+micro organisms وحاولت أقول لكم إيش يعني
+
+129
+00:09:58,090 --> 00:10:02,030
+medical microbiology لكن نركز عليها الآن بتركيز
+
+130
+00:10:02,030 --> 00:10:09,810
+أعلى، الـ medical microbes خلينا ننتقل للموضوع إحنا 
+
+131
+00:10:09,810 --> 00:10:17,750
+لو يعني كنا نائمين وصحينا من النوم الساعة مثلاً 
+
+132
+00:10:17,750 --> 00:10:23,830
+الثالثة الفجر، نحط إصبعنا قدامنا خاصة في ضل ضوء الكهرباء
+
+133
+00:10:23,830 --> 00:10:30,590
+مش هنشوف حاجة، خلينا صاحيين لساعة العاشرة الصبح تمام؟ العاشرة
+
+134
+00:10:30,590 --> 00:10:34,870
+الصبح، كيف يكون الجو؟ زي ما إحنا الآن، نهار، بين
+
+135
+00:10:34,870 --> 00:10:39,090
+الرابعة، الرابعة فجراً إلى العاشرة صباحاً، هل درجات
+
+136
+00:10:39,090 --> 00:10:45,420
+الظلمة والإنارة كانت واحدة؟ صرنا نشوف، لكن مش 
+
+137
+00:10:45,420 --> 00:10:49,780
+بدرجة واضحة، بعدين تدريجياً، وبعدين سنشوف بدرجة واضحة
+
+138
+00:10:49,780 --> 00:10:54,460
+إذا ما كانش في عندي ظلام دامس أو نور ساطع بين
+
+139
+00:10:54,460 --> 00:10:57,640
+الفترتين فقط، لا كان في عندي فترات ما فيش فيها وضع
+
+140
+00:10:57,640 --> 00:11:02,960
+ترقية، وهذا الكلام بدنا نحاول أن المفهوم هنا نغرّسه
+
+141
+00:11:02,960 --> 00:11:08,800
+فيكم لما نتكلم عن الـ medical microbes، ميكروب ممرض
+
+142
+00:11:08,800 --> 00:11:13,760
+وميكروب غير ممرض، نحط ذلك في الظلام الدامس
+
+143
+00:11:13,760 --> 00:11:18,940
+الممرض والغير ممرض في النور الصافية، لكن فيما بينهما
+
+144
+00:11:18,940 --> 00:11:23,900
+في ميكروبات قد تسبب أمراض، وميكروبات أخرى، أو نفس
+
+145
+00:11:23,900 --> 00:11:27,460
+الميكروب قد لا يسبب أمراض، بمعنى لو رشينا عليكم أن
+
+146
+00:11:27,460 --> 00:11:31,660
+الميكروب هذا الآن، جزء أمام البعض منكم ممكن يقول
+
+147
+00:11:31,660 --> 00:11:36,840
+البعض الآخر ما يمرضش، ليش؟ نفهم أن الممرض هو
+
+148
+00:11:36,840 --> 00:11:42,240
+absolute يعني مصطلح مطلق، وإنما مصطلح relative في
+
+149
+00:11:42,240 --> 00:11:47,220
+عوامل أخرى تتحكم فيه، في دائماً ميكروبات معروفة عنها
+
+150
+00:11:47,220 --> 00:11:51,880
+أنها pathogenic، وميكروبات أخرى non pathogenic
+
+151
+00:11:51,880 --> 00:11:54,300
+pathogenic يعني ممرضة، و non pathogenic يعني غير
+
+152
+00:11:54,300 --> 00:11:58,200
+ممرضة، في الـ medical microbiology غالباً هيتطرق
+
+153
+00:11:58,200 --> 00:12:02,720
+للنوعين، للممرضة وللغير ممرضة، لأنه يهمنا نعمل
+
+154
+00:12:02,720 --> 00:12:05,820
+differentiation، اللي هو عملية تمييز بين
+
+155
+00:12:08,580 --> 00:12:11,680
+أحد النقاط المهمة جداً في الـ medical microbial اللي
+
+156
+00:12:11,680 --> 00:12:14,140
+دراستوها في التنمية العالمية ودراستوها في الأحياء
+
+157
+00:12:14,140 --> 00:12:18,080
+تبع مستوى أول هو الفروقات بين الـ prokaryotes والـ
+
+158
+00:12:18,080 --> 00:12:22,740
+eukaryotes، طبعاً هأذكركم بالفرق الرئيسي عشان أنا بدي
+
+159
+00:12:22,740 --> 00:12:25,280
+آخذ منه بس من جدول هذا، بدي آخذ منه معلومة أو
+
+160
+00:12:25,280 --> 00:12:29,860
+معلومتين عشان أوصلكم بفكرة أساسية أن كيف بنستخدم أو
+
+161
+00:12:29,860 --> 00:12:33,240
+بنوظف الفروقات بين الـ prokaryotes والـ eukaryotes
+
+162
+00:12:33,240 --> 00:12:38,620
+حقيقية النواة يعني في إيها غشاء نوى وبداية النوى
+
+163
+00:12:38,620 --> 00:12:43,800
+أنا مالهاش غشاء النوى، هذا التعريف الأساسي، لكن فيه
+
+164
+00:12:43,800 --> 00:12:47,380
+فروقات أخرى كثيرة، شايفين الدول كبيرة، أنا بدي آخذ
+
+165
+00:12:47,380 --> 00:12:52,460
+فرق أساسي آخر وأنطلق منه، ريبوزوم، ما هي
+
+166
+00:12:52,460 --> 00:12:57,420
+وظيفة الريبوزوم؟ اسمها الـ protein، فاكرين الكورة
+
+167
+00:12:57,420 --> 00:13:00,660
+الكبيرة والكورة الصغيرة اللي هي الـ large subunit
+
+168
+00:13:00,660 --> 00:13:05,280
+والـ small subunit، في الإيوكاريوت الريبوزوم هذا
+
+169
+00:13:05,280 --> 00:13:12,350
+القطعتين بنسميهم 80S، 80 يعني ثمانين، أنا مش هأرسم
+
+170
+00:13:12,350 --> 00:13:20,390
+ثمانين اس، نعم بالكود
+
+171
+00:13:20,390 --> 00:13:25,930
+سامحوني أنا في الرسم يعني كثير قوي، فهتنبهروا من
+
+172
+00:13:25,930 --> 00:13:30,770
+الرسمات اللي برسمها، هاي أول دائرة، أعتقد هذه أحسن
+
+173
+00:13:30,770 --> 00:13:31,670
+دائرة اللي برسمها أنا
+
+174
+00:13:36,630 --> 00:13:42,910
+80S، كل ريبوزوم هذا لو حطيناه في جهاز ترسيب هيتّرسّب
+
+175
+00:13:42,910 --> 00:13:49,550
+على سرعة 80، سرعة 80، لو فصلناهم عن بعض قطعة قطعة
+
+176
+00:13:49,550 --> 00:13:53,410
+هذا الكبيرة وهذا الصغيرة، وحطينا الكبيرة في جهاز
+
+177
+00:13:53,410 --> 00:13:58,230
+الترسيب هيتّرسّب على 50S، وهذا هيتّرسّب على 40S، محدش 
+
+178
+00:13:58,230 --> 00:14:01,830
+مُولد يقول 60، 40، 100، لا، مش جمال العملية، العملية مش
+
+179
+00:14:01,830 --> 00:14:08,050
+جمال العملية، سرعة ترسيب كلّه على بعضه ترسب عن ثمانين
+
+180
+00:14:08,050 --> 00:14:12,890
+لما فصلناهم على ستين، هذا على أربعين، بينما الآخر
+
+181
+00:14:12,890 --> 00:14:15,930
+اللي هو اللي تبقى الـ bacterial، بروكاريوتز، اسمه 
+
+182
+00:14:15,930 --> 00:14:20,370
+70S، يعني كله ترسب على كثير على سبعين، الكبيرة
+
+183
+00:14:20,370 --> 00:14:24,110
+على خمسين، والصغيرة على ثلاثين، جمعناهم مع بعض أول
+
+184
+00:14:24,110 --> 00:14:27,630
+ما جمعناهم، مش هذه المعلومة اللي هأطلبها، الوحيد اللي
+
+185
+00:14:27,630 --> 00:14:32,750
+هتحفظوها من المحاضرة هذه، 70S و 80S
+
+186
+00:14:32,750 --> 00:14:37,780
+الثمانين، ستين، أربعين، والخمسين، ثلاثين، ثانية، ليش أنا
+
+187
+00:14:37,780 --> 00:14:43,160
+بطلب المعلومة دلوقت؟ أفهم، لأنه إذا إحنا عرفنا
+
+188
+00:14:43,160 --> 00:14:49,020
+المعلومات وحفظناها في أدمغتنا ولم نستخدمها فهي
+
+189
+00:14:49,020 --> 00:14:53,520
+عديمة النفع، لا علم إلا ما ينفع، لازم العلم يكون
+
+190
+00:14:53,520 --> 00:14:59,400
+نافع، لازم نستفيد منه، بناخد antibiotic لما بيصيبنا
+
+191
+00:14:59,400 --> 00:15:03,890
+التهاب، إحنا الـ antibiotic بيقضي على الـ protein
+
+192
+00:15:03,890 --> 00:15:06,550
+synthesis أو بيوقف الـ protein synthesis في الخلايا
+
+193
+00:15:06,550 --> 00:15:12,110
+لماذا يوقف الـ protein synthesis في البكتيريا ولا
+
+194
+00:15:12,110 --> 00:15:17,610
+يوقف الـ protein synthesis في خلايانا؟ لأنه بيستهدف
+
+195
+00:15:17,610 --> 00:15:23,590
+الـ 30S ribosomal subunit، الـ 30S في من موجود هذه؟
+
+196
+00:15:23,590 --> 00:15:27,510
+في البكتيريا، الـ prokaryotes، طب هل فينا إحنا، إحنا
+
+197
+00:15:27,510 --> 00:15:31,990
+إيه prokaryotes؟ هل فينا 30S؟ مش اللي إحنا إحنا 40S
+
+198
+00:15:33,350 --> 00:15:36,330
+استهدفنا.. استهدفنا أو الـ .. الـ .. الـ antibiotic
+
+199
+00:15:36,330 --> 00:15:41,350
+استهدف الـ 30S، ما استهدفش مين؟ طب ليش بيستهدف الـ
+
+200
+00:15:41,350 --> 00:15:43,990
+30S بيستهدفش الـ 40S؟ زي ما أنتم جئتم على الغرفة
+
+201
+00:15:43,990 --> 00:15:48,250
+هذه يا بنات، خاص نص ما جئتم لغرف ثانية للمحاضرة هذه
+
+202
+00:15:48,250 --> 00:15:52,810
+لأنكم معكم ورقة ومسموح لكم بالدخول في هذا الوقت
+
+203
+00:15:52,810 --> 00:15:57,250
+صح؟ الـ binding بين المركبات وبين الخلايا بيكون 
+
+204
+00:15:57,250 --> 00:16:00,650
+specific فبيكون فيه receptor للـ antibiotic على الـ
+
+205
+00:16:00,650 --> 00:16:05,590
+30S وما فيش receptor على الـ 40S، إذا هي استفادتنا من
+
+206
+00:16:05,590 --> 00:16:08,690
+معلومة اسمها فروقات ما بين الـ prokaryote
+
+207
+00:16:08,690 --> 00:16:13,850
+والإيوكاريوت، فارق آخر إحنا خلايانا كإيوكاريوت
+
+208
+00:16:13,850 --> 00:16:17,050
+مالناش cell wall صح؟ ولا إلينا cell wall؟ لو إلينا
+
+209
+00:16:17,050 --> 00:16:21,070
+cell wall كنا سنة زي الطاولة، خشب، مظبوط؟ بس
+
+210
+00:16:21,070 --> 00:16:23,590
+مالناش cell wall، البكتيريا إلها cell wall
+
+211
+00:16:23,590 --> 00:16:29,210
+البنسالين المشهور عالمياً بيقتل البكتيريا بإنه
+
+212
+00:16:29,210 --> 00:16:34,010
+بيستهدف الـ cell wall synthesis، بيوقف تصنيع الـ cell
+
+213
+00:16:34,010 --> 00:16:37,470
+wall، يبقى بيقتل البكتيريا بيقتلناش إحنا لأن إحنا 
+
+214
+00:16:37,470 --> 00:16:41,110
+أصلًا مالناش cell wall، إذا المعرفة دي والفروق بين
+
+215
+00:16:41,110 --> 00:16:44,890
+الـ eukaryote والـ prokaryote مهمة جداً لتطبيقات
+
+216
+00:16:44,890 --> 00:16:50,710
+مستقبلية كمان، فرق ثالث ومهم جداً اللي هو الـ
+
+217
+00:16:50,710 --> 00:16:56,030
+chromosome، أكام كروموزوم عند البشر؟ يعني 23 ضرب 2
+
+218
+00:16:56,030 --> 00:17:01,850
+أظن أنهم أزواج، لكن في البكتيريا it is only one
+
+219
+00:17:01,850 --> 00:17:06,790
+single chromosome، وهذا الـ chromosome بيقول
+
+220
+00:17:06,790 --> 00:17:12,330
+circular يعني ما لهوش طرف، بينما كروموزوماتنا إحنا
+
+221
+00:17:12,330 --> 00:17:17,410
+linear، خطية زي الجلم، الجلم له كام طرف؟ طرفين، بس
+
+222
+00:17:17,410 --> 00:17:22,610
+الدائرة ما لهاش ولا طرف، إيش ميزة اثنين وواحد؟ هاي
+
+223
+00:17:22,610 --> 00:17:28,610
+الكروموزوم، وهي المقابل إلها على الجهة الثانية، نفترض إنه
+
+224
+00:17:28,610 --> 00:17:33,670
+في جين، نفس الجين هيكون وين؟ في الجهة الثانية، هذا
+
+225
+00:17:33,670 --> 00:17:38,450
+الجين صار فيه خلل، طفرة، mutation، الجين الثاني الـ
+
+226
+00:17:38,450 --> 00:17:43,610
+expression تبعه ما زال قادر على إنتاج نفس المنتج
+
+227
+00:17:43,610 --> 00:17:47,910
+وبالتالي لن نشعر إنه أصلًا صار فيه خلل في هذا الجين
+
+228
+00:17:47,910 --> 00:17:52,010
+لأنه فيه جين بديل بينتج نفس الإنتاج، يبقى الطفرة في
+
+229
+00:17:52,010 --> 00:17:56,820
+هذه الحالة لم تظهر نتائجها، لكن بالبكتيريا it is
+
+230
+00:17:56,820 --> 00:18:02,240
+only one single chromosome، وبالتالي أي طفرة هتظهر
+
+231
+00:18:02,240 --> 00:18:07,260
+وطفرة هتكتر، نقطة مهمة، it is easy to make
+
+232
+00:18:07,260 --> 00:18:12,200
+mutations in bacteria، وبعض الأحيان إحنا بنلجأ
+
+233
+00:18:12,200 --> 00:18:17,540
+لعمل mutation في البكتيريا لتحسين إنتاج منتج معين
+
+234
+00:18:17,540 --> 00:18:23,740
+مثلاً في دراستنا أيام الـ .. الستير، كان فينا كطلاب نحاول
+
+235
+00:18:23,740 --> 00:18:27,560
+ننتج إنزيم اسمه Alpha Amylase، والله عزلنا بكتيريا
+
+236
+00:18:27,560 --> 00:18:33,200
+وتمام اسمها Bacillus، ووريناها للدكتورة اللي
+
+237
+00:18:33,200 --> 00:18:37,700
+بتشرف علينا، قالت كويسة بس إنتاجها قليل، بدنا نحسن
+
+238
+00:18:37,700 --> 00:18:41,280
+إنتاجها، طب كيف نحسن إنتاجها؟ جاءت تعملولها طفرات،
+
+239
+00:18:41,280 --> 00:18:46,420
+تعملولها طفرات، أنتم يعني خربوها، فخدنا البكتيريا
+
+240
+00:18:46,420 --> 00:18:50,990
+وعرضناها للـ ultra violet light، الـ ultraviolet
+
+241
+00:18:50,990 --> 00:18:54,890
+light تعمل mutations لفترات معينة زمنية، trial and
+
+242
+00:18:54,890 --> 00:18:59,810
+error، وفي الآخر حصلنا على سلالة بتنتج alpha
+
+243
+00:18:59,810 --> 00:19:03,850
+amylase بكميات ضخمة، بدون حساب، طبعاً الطفرة بالنسبة
+
+244
+00:19:03,850 --> 00:19:07,630
+لإن إحنا نبلي إيشلان كويسة، إن إحنا كبشر من وجهة
+
+245
+00:19:07,630 --> 00:19:11,190
+نظرنا، إحنا من وجهة نظر البكتيريا سيئة لأنها فقدت
+
+246
+00:19:11,190 --> 00:19:15,610
+السيطرة على إنتاج الـ alpha amylase هذا سيء للطاقة
+
+247
+00:19:15,610 --> 00:19:20,830
+بالنسبة لها، لكن في الآخر it is easy to make
+
+248
+00:19:20,830 --> 00:19:25,050
+mutations in bacteria، لكن عكس الإنسان يقول إنه ..
+
+249
+00:19:25,050 --> 00:19:27,310
+إنه .. لأنه الـ diploid بيه عنده زوج من
+
+250
+00:19:27,310 --> 00:19:39,970
+الكروموزومات، وبالتالي بيكون الوضع أسلوب طيب 
+
+251
+00:19:39,970 --> 00:19:43,230
+أنتوا بتعرفوا أنه الممالك تبع المخلوقات في التصنيف
+
+252
+00:19:43,230 --> 00:19:47,090
+احنا بنحب التصنيف البشري وأعتقد أنه ت .. تروح
+
+253
+00:19:47,090 --> 00:19:50,950
+المكتبة بعض الأحيان .. بعض الأحيان بنروح المكتبات،
+
+254
+00:19:50,950 --> 00:19:55,230
+بنلاقيش كوم كتب في المكتبات، بنلاقي أرفف وطوابق،
+
+255
+00:19:55,230 --> 00:19:57,990
+هذا طابق للمراجع باللغة الإنجليزية، للعلمية،
+
+256
+00:19:57,990 --> 00:20:01,910
+للطبية، للكيميا، للأحياء، مظلومة لتصنيفات تخيل لو
+
+257
+00:20:01,910 --> 00:20:07,070
+أنا بدي كتاب في الكيميا، وبروح لاجي كل كتب المكتبة
+
+258
+00:20:07,070 --> 00:20:10,710
+كومه معاهم، يمكن ما أحد ما بيديا يطلع الكتاب، ويمكن
+
+259
+00:20:10,710 --> 00:20:15,110
+يطلع آخر كتاب، صح ولا لأ؟ فنحن نميل إلى التصنيف
+
+260
+00:20:15,110 --> 00:20:20,450
+والترتيب بسهولة استرجاع المعلومة اسمها
+
+261
+00:20:20,450 --> 00:20:25,250
+retrieval system استرجاع المعلومات التصنيف مر في
+
+262
+00:20:25,250 --> 00:20:30,590
+مراحل كتيرة على المخلوقات وفيها لحد الآن عليها
+
+263
+00:20:30,590 --> 00:20:36,110
+خلافات، والخلافات هادي هادي عن تشابك المعلومات و
+
+264
+00:20:36,110 --> 00:20:41,410
+التغيير بتم لأن معلوماتنا بتزيد يعني بعض الأحيان
+
+265
+00:20:41,410 --> 00:20:45,710
+ما بنكتشفش مايكروفات جديدة ولا مخلوقات جديدة لكن
+
+266
+00:20:45,710 --> 00:20:48,720
+معلوماتنا بتزيد ليش معلوماتنا بتزيد؟ لأنه في
+
+267
+00:20:48,720 --> 00:20:52,940
+research في أبحاث ولأنه في تقنيات كنا زمان ب
+
+268
+00:20:52,940 --> 00:20:55,460
+microscope عادي بعدين صرنا ب electron microscope
+
+269
+00:20:55,460 --> 00:20:59,940
+بعدين صرنا ب confocal microscopy صرنا بتقنيات
+
+270
+00:20:59,940 --> 00:21:05,520
+متطورة جدا وبتالي زادت معلومات فالتصنيف ليس يعني
+
+271
+00:21:05,520 --> 00:21:09,040
+شيء fixed ممكن يتغير وهنشوف هذا الكلام في
+
+272
+00:21:09,040 --> 00:21:12,200
+البكتيريا بعض الأحيان لكن الأنيمال أمامنا ما تغيروا
+
+273
+00:21:12,200 --> 00:21:14,340
+دلوقتي متانية أمامنا ما تغيروا animal and plant
+
+274
+00:21:14,340 --> 00:21:19,180
+ميزوا بين الحيوانات وبين النباتات منذ زمن بعيد
+
+275
+00:21:19,180 --> 00:21:23,860
+البروتست إغلاق تشمل لبروتزوفة ومن الأشياء القريبة
+
+276
+00:21:23,860 --> 00:21:28,180
+الفنجاي اللي هي الفطريات مملكة لحالها البروكاريوت
+
+277
+00:21:28,180 --> 00:21:31,560
+عملوها مملكة وعملوا الفيروسات مملكة كم مملكة صاروا
+
+278
+00:21:31,560 --> 00:21:38,320
+هذولا؟ ستة واحد اثنين ثلاثة أربعة خمسة واحد ستة لكن
+
+279
+00:21:38,320 --> 00:21:42,940
+في شيء أعلى من المملكة أعلى من المملكة يسموه
+
+280
+00:21:42,940 --> 00:21:45,990
+domain ما في عندي domain eukaryote و eukaryote يعني
+
+281
+00:21:45,990 --> 00:21:47,970
+الـ eukaryotes لحظوا مين تحت الـ eukaryotes
+
+282
+00:21:47,970 --> 00:21:54,930
+animal, plant, protist و fungi تحت ال .. البكتيريا
+
+283
+00:21:54,930 --> 00:22:01,590
+في دمية البكتيريا أو الاركيا الوكاريوتس فقط مملكة
+
+284
+00:22:01,590 --> 00:22:07,530
+واحدة وال viruses ليست .. ليست ضمن تصنيف ال
+
+285
+00:22:07,530 --> 00:22:11,510
+domains مملكة ما لهاش domain، مملكة ما لهاش domain
+
+286
+00:22:12,670 --> 00:22:17,070
+لكن اللي بيوصل ان كل هذه المخلوقات اللي على كوكب الأرض
+
+287
+00:22:17,070 --> 00:22:20,410
+تصنف عليها Eukaryotes وده ال mainly فقط ال
+
+288
+00:22:20,410 --> 00:22:23,130
+bacteria و ال archaea أول مرة يمول معنا مصطلح
+
+289
+00:22:23,130 --> 00:22:29,870
+archaea بنحاول نوضح مين بيعرف إيش معنى عالم آثار؟
+
+290
+00:22:29,870 --> 00:22:37,730
+لغة لإنجليزية عالم آثار archaeologist archaeology
+
+291
+00:22:37,730 --> 00:22:42,770
+يعني علم الآثار والآثار غالبا بتكون مستقبلية قديمة
+
+292
+00:22:42,770 --> 00:22:47,830
+.. قديمة فسمى للبكتيريا Archaia لأن العلماء بتوع
+
+293
+00:22:47,830 --> 00:22:53,030
+التصنيف وبتوع التطور تمام بيقولوا إنها هي الأقدم
+
+294
+00:22:53,030 --> 00:22:56,230
+في السلسلة احنا طبعا كمسلمين وأنتوا عارفين أن ..
+
+295
+00:22:56,230 --> 00:23:00,670
+أن .. أن .. معتقدين أن الله سبحانه وتعالى هو خلق كل
+
+296
+00:23:00,670 --> 00:23:06,210
+هذه المخلوقات مش خلق بالتطور من إلى .. من إلى ال
+
+297
+00:23:06,210 --> 00:23:11,090
+Archaia قلنا أنه في التصنيف بيسموها البكتيريا
+
+298
+00:23:11,090 --> 00:23:16,220
+القديمة لكن بتعطيكم بعض الأمثلة على الاركيا وعلى
+
+299
+00:23:16,220 --> 00:23:21,080
+روعتها في هذا العالم بتعرفوا البحر الميت؟ لسه مو
+
+300
+00:23:21,080 --> 00:23:27,880
+بحر ميت ما فيش فيه حياة صح؟ اليوم بتقولوا أن البحر
+
+301
+00:23:27,880 --> 00:23:32,680
+الميت ظلم وسمي بحر ميت على .. على .. لدفس السيطرة
+
+302
+00:23:32,680 --> 00:23:36,660
+هو ده لسه ميت لأنه بتحيا فيه بكتيريا من الاركيا
+
+303
+00:23:36,660 --> 00:23:43,140
+اسمها halobacterium halopium وكلمة halo ملحهاله
+
+304
+00:23:43,140 --> 00:23:47,720
+معناها ملح فهي محبة للملح وبتعيش في البحر المقلور
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+305
+00:23:47,720 --> 00:23:52,040
+بناها عندنا وحاطناها في البحر تبعنا بتموت بدها ملح
+
+306
+00:23:52,040 --> 00:23:58,820
+تمام؟ بدها كمية كبيرة من الملح لو تعرفوا في يانبيه
+
+307
+00:23:58,820 --> 00:24:02,660
+مياه سخنة ساخنة قد تصل درجة الحرارة فيها إلى تسعين
+
+308
+00:24:02,660 --> 00:24:07,000
+إلى خمس وتسعين درجة مئوية هناك تعيش بكتيريا من
+
+309
+00:24:07,000 --> 00:24:15,360
+نوع اركيا هناك كهوف كهوف في جبال بيرشح منها acid
+
+310
+00:24:15,360 --> 00:24:21,080
+مادة حمضية sulfuric acid H2SO4 لو صابت جلدنا
+
+311
+00:24:21,080 --> 00:24:27,320
+هتحرقه هنا تعيش بكتيريا تنتج هذا الحمض من نوع مين؟
+
+312
+00:24:27,320 --> 00:24:32,140
+من نوع Archa لذلك بعض العلماء بيطلقوا عليها
+
+313
+00:24:32,140 --> 00:24:39,080
+extremophile يعني البكتيريا المحبة للظروف المتطرفة،
+
+314
+00:24:39,080 --> 00:24:42,040
+البحر الميت بلوحة عالية، الحرارة العالية جدا اللي
+
+315
+00:24:42,040 --> 00:24:45,520
+بيقولون عنها في ال springs، الحموضة المرتفعة اللي
+
+316
+00:24:45,520 --> 00:24:49,360
+اتكلمنا عنها، إذا ال .. ال .. ال .. ال Archa هي
+
+317
+00:24:49,360 --> 00:24:53,560
+نوع من البكتيريا لكن غالبا بتعيش هنا في ظروف ..
+
+318
+00:24:53,560 --> 00:24:57,900
+فبتالي مش .. مش هلاقي Archa ضمن اللي هو ال medical
+
+319
+00:24:57,900 --> 00:25:00,920
+microbiome، مش هلاقي Archa ضمن ال medical
+
+320
+00:25:00,920 --> 00:25:03,760
+microbiome، هلاقي مين؟ فقط بكتيريا
+
+321
+00:25:07,860 --> 00:25:13,540
+أين نتوقع أن نجد micro organism؟ أكيد لكم أحد
+
+322
+00:25:13,540 --> 00:25:17,560
+موبايلز أه؟ فصل الماضي عملنا دراسة على ..
+
+323
+00:25:17,560 --> 00:25:20,920
+زميلاتكم في القسم عملوا دراسة على ال mobile phone
+
+324
+00:25:20,920 --> 00:25:26,360
+وأظهروا نتائج يعني سيئة للغاية طبعا الإناث كانوا
+
+325
+00:25:26,360 --> 00:25:31,640
+أفضل من الذكور في المحافظة على نظافة ال mobile لكن
+
+326
+00:25:31,640 --> 00:25:35,270
+بشكل عام لجينا نسبة تتجاوز ال 50% من موبايلات
+
+327
+00:25:35,270 --> 00:25:41,330
+الطلبات تحتوي على potential pathogens يعني ممرضات
+
+328
+00:25:41,330 --> 00:25:48,930
+محتملة طيب بلاش نسأل السؤال بطريقة ثانية عطوني
+
+329
+00:25:48,930 --> 00:25:56,410
+مكان بتعرفوا عنه أنتم محقق الإجابة
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+330
+00:25:56,410 --> 00:25:56,870
+بعيدة
+
+331
+00:26:00,400 --> 00:26:06,000
+أبطل عمليات هل تعقيم طبيعي ولا تعقيم صناعي تعقيم
+
+332
+00:26:06,000 --> 00:26:09,640
+الصناعي فإن حالة تعقيم طبيعي حالة معقمة يعني مثلا
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+333
+00:26:09,640 --> 00:26:18,120
+فاكهة أكلتوها فكهة معقمة ال exterior تبع الأشياء
+
+334
+00:26:18,120 --> 00:26:21,560
+لازم يكون عليها micro wave يعني لو أخدنا منكم
+
+335
+00:26:21,560 --> 00:26:25,160
+مسحات من إيدكم وأنتم مغسلين وجايين لسه علاجة
+
+336
+00:26:25,160 --> 00:26:30,300
+micro wave تفاحة من برة مش فيها microorganism؟ فيها
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+337
+00:26:30,300 --> 00:26:34,180
+microorganism أفضل من جوا ما فيها شيء احنا أجسامنا
+
+338
+00:26:34,180 --> 00:26:39,620
+الجلد تبعنا الأنف الفم مليء بال microorganism ال
+
+339
+00:26:39,620 --> 00:26:44,400
+intestinal tract مليئة بال microorganism لكن الدم
+
+340
+00:26:44,400 --> 00:26:51,520
+تبعنا ال CSF يبقى في جسمنا احنا بنقدر نقسمه لجسمين
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+341
+00:26:51,520 --> 00:26:56,960
+وهذا على فكرة أسهل تقسيمة في كل العلوم اللي
+
+342
+00:26:56,960 --> 00:27:00,140
+بتدرسوها في حياتكم في الإنسان بنقسمه لجسمين
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+343
+00:27:00,140 --> 00:27:04,940
+sterile و non-sterile parts أعضاء أو أجزاء معقمة
+
+344
+00:27:04,940 --> 00:27:07,420
+طبعا هذا تقسيم مهم لدني ليش لأن لو أنا صار عن
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+345
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+التهاب في الأنف وبدي أخد مسحة من الأنف عشان أشوف
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+346
+00:27:11,160 --> 00:27:15,500
+المسبب للاتهاب الميكروب اللي طلع معايا الآن هل هو
+
+347
+00:27:15,500 --> 00:27:20,700
+من هو المسبب للاتهاب ولا من الموجودات الطبيعية
+
+348
+00:27:20,700 --> 00:27:23,600
+اللي بنسميها normal flora أو normal microbiota مش
+
+349
+00:27:23,600 --> 00:27:28,530
+عارفين ما بنعرفش صح؟ هذه صعوبة فبنلاقي طرق للتفريق
+
+350
+00:27:28,530 --> 00:27:33,870
+بين الاثنين أمامنا ال microbes are everywhere و
+
+351
+00:27:33,870 --> 00:27:38,670
+اللي قالت الأخت اللي قالت أنه غرفة العمليات بنصرف
+
+352
+00:27:38,670 --> 00:27:42,490
+مصاريف كتير وفلوس كتير عشان نوفر ظروف معقمة في غرفة
+
+353
+00:27:42,490 --> 00:27:48,170
+العمليات يعني يكلفنا الأمر فلوس حتى نتخلص من ال
+
+354
+00:27:48,170 --> 00:27:53,130
+microorganism فالميكروبات دائما موجودة وما أنها
+
+355
+00:27:53,130 --> 00:27:56,550
+موجودة في كل مكان ال air و ال soil و ال water و ال
+
+356
+00:27:56,550 --> 00:28:00,430
+animals و ال human body بنلاقي واحنا بنشتغل
+
+357
+00:28:00,430 --> 00:28:05,450
+microbiology في المعامل بنلاقي طريقة ما نلوثش
+
+358
+00:28:05,450 --> 00:28:12,430
+العينات مننا احنا ما نتكلمش إذا أنا أكون مضاف
+
+359
+00:28:12,430 --> 00:28:17,110
+مطهرين تمام نشتغل جنب اللهب ما نخلش الهوى اللي
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+360
+00:28:17,110 --> 00:28:24,180
+موجود ينزل ما نتكلمش ما نتنفسش في الطبخ تبعنا تبعا كل
+
+361
+00:28:24,180 --> 00:28:28,320
+هذه أشياء ممارسات يجب أن نتعلمها في المعمل حتى
+
+362
+00:28:28,320 --> 00:28:34,620
+نتجنب أن نطلع نتائج خاطئة في ال microbiology طبعا
+
+363
+00:28:34,620 --> 00:28:38,080
+أنا بقول دائما أن ال soil هي المصدر الأساسي للتربة
+
+364
+00:28:38,080 --> 00:28:43,180
+لكن من ال soil تعرف أن الهواء بيلمس التربة بياخد
+
+365
+00:28:43,180 --> 00:28:46,880
+معاها غبار وغبار في أين بيسمع أن؟ الهواء الهواء
+
+366
+00:28:46,880 --> 00:28:53,910
+لا يوفر بيئة ده يعني رطوبة مناسبة أو تغذية مناسبة
+
+367
+00:28:53,910 --> 00:28:57,690
+فتالي هو مجرد carrier للميكروبات حامل للميكروبات
+
+368
+00:28:57,690 --> 00:29:02,210
+ينقلها من مكان إلى مكان بس التربة فيها مادة عضوية
+
+369
+00:29:02,210 --> 00:29:06,090
+وفيها رطوبة قد توفر بيه ألمين لنمو المايكرو
+
+370
+00:29:06,090 --> 00:29:12,270
+أورجانز ال water مش تشتت دنيا تاخد معاها تربة و
+
+371
+00:29:12,270 --> 00:29:16,070
+تروح عليها بشكل streams و تروح على نهار أو على
+
+372
+00:29:16,070 --> 00:29:19,130
+البحار ال animals و ال human bodies احنا في ال
+
+373
+00:29:19,130 --> 00:29:24,650
+intestinal tract بتاعنا one gram one gram of stool
+
+374
+00:29:24,650 --> 00:29:30,250
+contains ten to the power of nine او twelve يعني
+
+375
+00:29:30,250 --> 00:29:34,010
+عشرة أُس تسعة لعشرة أُس اثنا عشر micro organism ده كل 
+
+376
+00:29:34,010 --> 00:29:38,810
+ايش رأيك في الرقم؟ رقم مهول  الـ saliva اللي
+
+377
+00:29:38,810 --> 00:29:43,970
+بتاع الإنسان الواحد ملي منه بيحتوي على عشرة
+
+378
+00:29:43,970 --> 00:29:48,190
+مليون تسعة micro organism  بتاع التقولي فرشية أسناني
+
+379
+00:29:48,190 --> 00:29:52,310
+فرشية أسناني أه؟ أصلا الناس المهووسين بأنهم
+
+380
+00:29:52,310 --> 00:29:55,250
+يفرشوا أسنانهم باستمرار بيصيروا عندهم أمراض يعني
+
+381
+00:29:55,250 --> 00:29:58,950
+الفم يجب أن يحتوي على micro organism يجب أن يحتوي
+
+382
+00:29:58,950 --> 00:30:03,430
+على .. مش معناه أضحك أنه نسبه عفن لأ المقصود فيه
+
+383
+00:30:03,430 --> 00:30:08,370
+أنه يعني لا مبالغة ولا تتفرض في الـ .. فالـ human
+
+384
+00:30:08,370 --> 00:30:11,810
+body و الـ animal body بتحتوي على micro organism
+
+385
+00:30:11,810 --> 00:30:17,790
+بعدد ضخمة جدا هذا مثال على garden soil بيقولنا
+
+386
+00:30:17,790 --> 00:30:21,230
+محتوى على تسعة أو سبعة من عشرة ضرب عشرة أو إذا كده
+
+387
+00:30:21,230 --> 00:30:26,410
+مليونين ممكن ستة أو سبعة micro organism من التربة
+
+388
+00:30:26,410 --> 00:30:31,790
+اللي بتاعت المنزل طبعا ايش الميزة ما بين تربة و ما
+
+389
+00:30:31,790 --> 00:30:37,090
+بين إنسان أو أو أو انسان مصعب بداية بنفهم مصطلح
+
+390
+00:30:37,090 --> 00:30:40,950
+كتير مهم اسمه competition الآن أنتم في الفصل هنا
+
+391
+00:30:40,950 --> 00:30:46,170
+لو كنتم عشر أشخاص فرصتكم في أنكم تسألوا سؤال ايه
+
+392
+00:30:46,170 --> 00:30:50,470
+ليه واحد من عشرة صح ولا لأ فرصة أي واحدة منكم طب
+
+393
+00:30:50,470 --> 00:30:54,250
+لو زاد العدد  صار خمسين بيصير فرصتك واحد من خمسين
+
+394
+00:30:54,250 --> 00:31:01,630
+فالمنافسة بتزيد فبالتالي أنت معرفتك بتجل معرفتك
+
+395
+00:31:01,630 --> 00:31:06,510
+بتجل فوق أقصد فرصك بتجل في الـ .. في البيئة
+
+396
+00:31:06,510 --> 00:31:12,130
+الحقيقية الميكروبات بتكون في حالة تنافس ميكروب
+
+397
+00:31:12,130 --> 00:31:16,190
+وميكروب وميكروب وميكروب بتنفسوا مع بعض وكل ميكروب
+
+398
+00:31:16,190 --> 00:31:19,490
+فيه استراتيجياته في الدفاع واستراتيجياته في
+
+399
+00:31:19,490 --> 00:31:25,730
+الهجوم لكن لو واحد من هذه الميكروبات أخذناه لحاله
+
+400
+00:31:25,730 --> 00:31:30,390
+من المجموعة وحطيناه في بيئة ما فيش فيها تنافس خرصه
+
+401
+00:31:30,390 --> 00:31:34,430
+بتزيد أو بتزيد ايش؟ بتزيد وبالتالي احنا بنحاول برضه
+
+402
+00:31:34,430 --> 00:31:38,910
+لما بنقزل ميكروب من environment أو من clinical
+
+403
+00:31:38,910 --> 00:31:44,930
+sample أنه ينشيل عنه الآخرين عشان نوفر له فرصة عالية
+
+404
+00:31:44,930 --> 00:31:48,170
+فالـ competition بتكون أقل في الحالة والفرصة بتكون
+
+405
+00:31:48,170 --> 00:31:54,290
+أفضل هذه الـ .. هذه المادج على أنه أرقام البكتيريا
+
+406
+00:31:54,290 --> 00:31:58,190
+بتتراوح بحسب المادة اللي موجودة فيها مثلا أنا عندي
+
+407
+00:31:58,190 --> 00:32:04,570
+الـ cheese 
+
+408
+00:32:04,570 --> 00:32:08,970
+الـ milk طبعا الـ milk الـ raw milk اللي مش مبسطة قد يصل
+
+409
+00:32:08,970 --> 00:32:13,230
+فيها من 1¹⁸ لكل جرام لكن بعد ما نبسطره ونبسطره
+
+410
+00:32:13,230 --> 00:32:16,350
+ليست عملية تعقيم خدوا بالكم وإنما عملية قتل للبعض
+
+411
+00:32:16,350 --> 00:32:19,710
+من الـ micro organism لذا أنا في اندي تراوح و
+
+412
+00:32:19,710 --> 00:32:24,010
+تثاؤت في الـ micro organism من surface إلى surface
+
+413
+00:32:24,010 --> 00:32:29,930
+طيب قبل ما أبدأ أتكلم باللوحة الفيكوسية اللي قطعت
+
+414
+00:32:29,930 --> 00:32:33,510
+عنها وما أتكلم عنها بدنا نحاول الآن نرجع إليها
+
+415
+00:32:34,710 --> 00:32:37,770
+وقلنا أن الـ micro organism are with great
+
+416
+00:32:37,770 --> 00:32:43,670
+biodiversity ايش يعني biodiversity؟ تنوع حيوي
+
+417
+00:32:43,670 --> 00:32:48,510
+مهول، بدنا نستغل هذه المعلومة نعرفها وشو هي تنوع
+
+418
+00:32:48,510 --> 00:32:51,450
+حيوي وايه يعني ممكن أو كيف ممكن نستغل هذه
+
+419
+00:32:51,450 --> 00:32:57,950
+المعلومة؟ احنا على المستوى البشري طعامنا اللي
+
+420
+00:32:57,950 --> 00:33:01,230
+قدامنا اللي بنشوفه إما نباتات أو حيوانات صح هذا
+
+421
+00:33:01,230 --> 00:33:06,900
+لأ؟ وطبعا أصناف محددة منه مش كل شيء كده المايكروبات
+
+422
+00:33:06,900 --> 00:33:11,840
+لما أنا قلت إنها موجودة everywhere في البحر الميت
+
+423
+00:33:11,840 --> 00:33:16,660
+وفي الكهوف الحمضية وفي الميه أقولكم ابتدعوا الميه
+
+424
+00:33:16,660 --> 00:33:21,140
+المعدنية اللي بيشربوا هذه لما فيها مايكروبات أنا مش
+
+425
+00:33:21,140 --> 00:33:24,600
+بنقولكم أن كل .. كل العالم موجود كتير .. قلتلكم
+
+426
+00:33:24,600 --> 00:33:28,760
+قلت 98% من المايكروبات أصلا مش ضار يعني 2% فقط في
+
+427
+00:33:28,760 --> 00:33:33,370
+الماء 1% من المايكروبات هو اللي ضار لكن وجود
+
+428
+00:33:33,370 --> 00:33:36,730
+المايكروبات هذه في بيئات متنوعة ومختلفة ماذا يعني
+
+429
+00:33:36,730 --> 00:33:43,270
+لكم؟ يعني مقدرة هذه الميكروبات المتنوعة على تناول
+
+430
+00:33:43,270 --> 00:33:50,190
+أغذية متنوعة طب أقولكم مثال مضحك جدا في بكتيريا
+
+431
+00:33:50,190 --> 00:33:54,710
+بتعيش في درجة حرارة قد تصل إلى 200 درجة مئوية وين
+
+432
+00:33:54,710 --> 00:33:57,570
+في عصرها الأرض 200 درجة مئوية؟ في الشيء تحت قاع
+
+433
+00:33:57,570 --> 00:34:04,000
+المحيطات بتشوفوا إن كان في الـ .. في الوثائقيات شيء
+
+434
+00:34:04,000 --> 00:34:07,740
+بيطلع من تحت المحيط بيسموها thermal vents فتحات هي
+
+435
+00:34:07,740 --> 00:34:11,760
+عبارة عن فتحات بركانية اللي .. اللي بيطلع منها
+
+436
+00:34:11,760 --> 00:34:15,620
+بيكون طبعا ساخن جدا ولإن المحيط الـ .. الـ column
+
+437
+00:34:15,620 --> 00:34:19,400
+بتاع الماء عالي جدا فبشكل ضغط فالماء قد يصل درجة حرارة
+
+438
+00:34:19,400 --> 00:34:22,460
+هناك فارق المنطقة إلى 200 درجة أو .. أو حتى أعلى
+
+439
+00:34:22,460 --> 00:34:27,360
+من ذلك ولا يوجد أي نوع من الضوء حتى نقول أن الـ
+
+440
+00:34:27,360 --> 00:34:30,100
+bacterium تم الـ photosynthesis اللي هو تمثيل ضوء
+
+441
+00:34:30,100 --> 00:34:35,860
+وإنما تقتات تتغذى على الكبريت الخارج من هذه
+
+442
+00:34:35,860 --> 00:34:42,060
+الفتحات بس على الكبريت باشي sun light باشي sun
+
+443
+00:34:42,060 --> 00:34:47,380
+light إذا تنوع التغذية في البكتيريا من نوع إلى آخر
+
+444
+00:34:47,380 --> 00:34:51,120
+تمام قد يفيدنا كيف بيفيدنا؟ في التعرف والتمييز
+
+445
+00:34:51,120 --> 00:34:55,840
+بينهم بتستخدمي واحد اثنين ثلاثة يا بتستخدم واحد
+
+446
+00:34:55,840 --> 00:35:00,400
+وأربعة وسبعة يا بتستخدم ثلاثة واثنين وواحد يبقى
+
+447
+00:35:00,400 --> 00:35:03,500
+بقدر أميزكم عن بعض دي الحلقة أبقولكم كيف الفكرة
+
+448
+00:35:03,500 --> 00:35:07,440
+بتاعتها تحت الـ microscope اسموا الأسماء اللي بتحكيها
+
+449
+00:35:07,440 --> 00:35:12,680
+مش للحفظ وإنما للمثال Salmonella type,
+
+450
+00:35:12,820 --> 00:35:16,580
+Escherichia coli, Sodomonas aeruginosa, Shigella,
+
+451
+00:35:18,080 --> 00:35:22,410
+Proteus, Morganella, Edward Sella اسمها حلوة بعد
+
+452
+00:35:22,410 --> 00:35:25,210
+فترة هتعودوا عليها وهتسموا بناتكم زيها في
+
+453
+00:35:25,210 --> 00:35:31,250
+المستقبل كل الأسماء اللي ذكرتهم وجابتهم متين اسم
+
+454
+00:35:31,250 --> 00:35:36,550
+تحت الميكروسكوب يبدو وكأنهم شحطة حمراء بقى زي ..
+
+455
+00:35:36,550 --> 00:35:44,090
+لونها أحمر وعصايا حمراء كيف نميز إيشريشيا كولار
+
+456
+00:35:44,090 --> 00:35:46,290
+عن سلمونيلا عن شيجيلا عن الأسماء اللي احنا
+
+457
+00:35:46,290 --> 00:35:50,860
+ذكرناهم؟ نستطيع تمييزهم من خلال الـ biodiversity من
+
+458
+00:35:50,860 --> 00:35:54,020
+خلال نموها هون في التغيير زي ما قلنا لكم أشهر
+
+459
+00:35:54,020 --> 00:35:57,480
+بيخمر الـ lactose أشهر بيخمرش الـ lactose أشهر
+
+460
+00:35:57,480 --> 00:36:01,880
+بيستخدم الـ glucose أشهر بيستخدم الـ sucrose وهكذا
+
+461
+00:36:01,880 --> 00:36:06,000
+يعني بندير على الفروقات في الـ metabolism وهذا
+
+462
+00:36:06,000 --> 00:36:08,940
+هيشكل جزء كبير من العمل بتاعكم السنة هذه إن شاء
+
+463
+00:36:08,940 --> 00:36:12,060
+الله هتعرفوا على كيف بنميز الـ micro organism
+
+464
+00:36:12,060 --> 00:36:15,260
+بالفحوصات اللي بيروح كيميائية طبعا هنسهرنا برضه الـ
+
+465
+00:36:15,260 --> 00:36:18,860
+ground state إن إن تشوفوا البكتيريا شحطة حمراء ليه؟
+
+466
+00:36:18,860 --> 00:36:21,820
+لكي تعرفوا أن هذه واحدة من هذه المجموعات واحدة
+
+467
+00:36:21,820 --> 00:36:23,440
+من هذه المجموعة.
+
+468
+00:36:29,420 --> 00:36:33,700
+هذه يمكن اللوحة اللي قدامنا بتوضح لنا دور الـ micro
+
+469
+00:36:33,700 --> 00:36:36,960
+organism في الـ nitrogen cycle، nitrogen cycle،
+
+470
+00:36:36,960 --> 00:36:40,400
+اللي هي دائرة الـ nitrogen. أنا بقولكم من بداية
+
+471
+00:36:40,400 --> 00:36:44,980
+المحاضرة إن تدوير العناصر تدوير العناصر هو الجزء
+
+472
+00:36:44,980 --> 00:36:49,120
+الأساسي من مهام الـ micro organism وأقولكم جملة
+
+473
+00:36:49,120 --> 00:36:52,520
+أخبرنا بيها أحد الدكاترة اللي درسونا احنا فيبقى
+
+474
+00:36:52,520 --> 00:36:56,480
+ننقلها لكم إن شاء الله كلمة لطيفة ولذيذة بتعبر عن
+
+475
+00:36:56,480 --> 00:36:59,580
+الواقع اللي بنقدر نقصد فيه الـ micro organism
+
+476
+00:36:59,580 --> 00:37:05,080
+بيقولوا لا تظلموا الميكرو بقى هذه المقدمة بتاعتكم
+
+477
+00:37:05,080 --> 00:37:09,020
+لا تظلموا الميكرو بقى their role in nature is to
+
+478
+00:37:09,020 --> 00:37:13,280
+convert large molecules into small molecules دورها
+
+479
+00:37:13,280 --> 00:37:16,900
+في الحياة دورة الحياة أن تحول الجزيئات الكبيرة
+
+480
+00:37:16,900 --> 00:37:21,300
+لجزيئات صغيرة doing their thing يعني doing their
+
+481
+00:37:21,300 --> 00:37:26,780
+job يعني خلال عملها they may fall in our food and
+
+482
+00:37:26,780 --> 00:37:32,320
+spoil it قد تقع مش .. مش جاسدها هي بس إذا هي قعدت
+
+483
+00:37:32,320 --> 00:37:36,200
+وينما .. وينما نحطها ابتدوا ويتكسر انتهى الموضوع
+
+484
+00:37:36,200 --> 00:37:40,340
+هي دورة الحياة if they enter our body they might
+
+485
+00:37:40,340 --> 00:37:44,460
+cause diseases إذا لا تظلموا الميكروفات تعمل عملها
+
+486
+00:37:44,460 --> 00:37:50,500
+وهي تحويل الجزيئات الكبيرة إلى جزيئات صغيرة فهذه
+
+487
+00:37:50,500 --> 00:37:54,220
+نقطة كتير مهمة وهذه طبعا أنا مش مش مش كتير مهتمين
+
+488
+00:37:54,220 --> 00:37:56,820
+بأنه كنا نعرف كيف الـ nitrogen سجل لكن في الآخر هي
+
+489
+00:37:56,820 --> 00:38:01,660
+عملية تحلل كنت عارف إن الميكروفات كمان ابتغى من
+
+490
+00:38:01,660 --> 00:38:05,800
+nitrogen fixation يعني
+
+491
+00:38:05,800 --> 00:38:10,860
+الزراعة استفادت بشكل كبير من هذه الحقيقة غير
+
+492
+00:38:10,860 --> 00:38:13,960
+البكتيريا اللي بتكون موجودة في الجذور اللي أصلا
+
+493
+00:38:13,960 --> 00:38:17,900
+درستوها نباتات دواة الجذور العقدية اللي بيكون
+
+494
+00:38:17,900 --> 00:38:22,040
+فيها عقد وهذا العقد أبقى عنها عن بكتيريا عايشة جوا
+
+495
+00:38:22,040 --> 00:38:25,420
+هذه العقد بعلاقة بيسميها symbiosis اللي هي علاقة
+
+496
+00:38:25,420 --> 00:38:30,760
+تكافلية بين النبات وبين البكتيريا هناك free living
+
+497
+00:38:30,760 --> 00:38:34,400
+bacteria ممكن تثبت الـ nitrogen لو نميناها في
+
+498
+00:38:34,400 --> 00:38:38,740
+المختبر ورشيناها على أراضي زراعية مش احنا هو كأنه
+
+499
+00:38:38,740 --> 00:38:42,750
+أرفنا سماد عضوي عليها يعني بمعنى آخر أن البكتيريا
+
+500
+00:38:42,750 --> 00:38:48,470
+لها دورة .. دورة عظيمة في هذا الموضوع لكن برضه 
+
+501
+00:38:48,470 --> 00:38:52,850
+شايفين أن هذا .. هذا عبارة عن necrosis قدم للمريض
+
+502
+00:38:52,850 --> 00:38:59,390
+وفي أن صار فيها اللي هو تحلل أو تاكل أو موضوع إيه
+
+503
+00:38:59,390 --> 00:39:03,430
+الخلايا فهذا الهرمش بيقولنا not all bacteria cause
+
+504
+00:39:03,430 --> 00:39:06,550
+disease الوزر أعظم اللي هو قاعة الهرم كلها non
+
+505
+00:39:06,550 --> 00:39:10,570
+pathogenic غير مفيدةالبعض منها potentially زي ما
+
+506
+00:39:10,570 --> 00:39:15,570
+قلتلكوا ممكن يمرض ممكن ما يمرضش والبعض الأخر موصوم
+
+507
+00:39:15,570 --> 00:39:23,730
+عليه علامة أن هذا دائما ممرض نعرف
+
+508
+00:39:23,730 --> 00:39:26,250
+كلمة مرة لأ بكافة طب اللي خليني أتكلم عن التعريف
+
+509
+00:39:26,250 --> 00:39:33,450
+يعني بشكل تفصيل شوية كلمة evolution أحنا قلتلكوا
+
+510
+00:39:33,450 --> 00:39:38,760
+مش متفقين فيها أن هو موضوع تطور classification لأ
+
+511
+00:39:38,760 --> 00:39:41,040
+متفقين في الـ classification التصنيف كتير مهم من
+
+512
+00:39:41,040 --> 00:39:44,800
+التصنيف ليش تصنيف مهم التصنيف مهم قولنالكوا اللي
+
+513
+00:39:44,800 --> 00:39:51,580
+غرض استرجاع المعلومة طالع لي هنا عندي شخص دخل
+
+514
+00:39:51,580 --> 00:39:54,820
+الباب وجاء دكتور فيه طالب من طالباتك أخذت معاه
+
+515
+00:39:54,820 --> 00:39:58,560
+مني ورقة وأنا مش هعرفها مش هعرف أي سنة طب
+
+516
+00:39:58,560 --> 00:40:03,900
+وصفينيها جاتيها طالبة بطلعتكوا بيقولكوا ليه طالبات
+
+517
+00:40:03,900 --> 00:40:06,060
+فبالتالي
+
+518
+00:40:07,100 --> 00:40:10,920
+أعطتني معلومة أكدتلي أنه أنا بدوّف المكان الصحيح
+
+519
+00:40:10,920 --> 00:40:16,840
+رقم اثنين إيش كمان ماجات لابسها تلبس نظارة الآن
+
+520
+00:40:16,840 --> 00:40:22,140
+أنا بدوّف عاملها على لابسين النظارة وبستخني الجميع
+
+521
+00:40:22,140 --> 00:40:27,600
+لآخر أكملّه حضر واحد اثنين ثلاثة ثلاثة استثنين
+
+522
+00:40:27,600 --> 00:40:33,320
+الأربعة استثنين الباقي طب كمان معلومة بتبقى تعطيني
+
+523
+00:40:33,320 --> 00:40:38,260
+معلومات لما أصل للشخص الصحيح الآن أنا شايف good DNA
+
+524
+00:40:38,260 --> 00:40:43,280
+صح بس الـ bacteria بشوفهاش good DNA بشوفها بذاكرتها
+
+525
+00:40:43,280 --> 00:40:50,060
+بمعنى بتجمعلها معلومات معلومات نظرية بصفاتها يعني
+
+526
+00:40:50,060 --> 00:40:51,960
+من آخر الفصل اللي جاي في الـ diagnostic
+
+527
+00:40:51,960 --> 00:40:55,960
+microbiology هتحفظوا إيه شوكولايك كرام positive ولا
+
+528
+00:40:55,960 --> 00:40:59,980
+كرام negative بس اللي ولا كوكسايد إجليكوز negative
+
+529
+00:40:59,980 --> 00:41:03,040
+ولا إجليكوز positive لا الأكتوز positive ولا
+
+530
+00:41:03,040 --> 00:41:08,670
+الأكتوز negative إن دول ميتيل ريدف و بروسكاوه أسماء
+
+531
+00:41:08,670 --> 00:41:13,950
+عديدة صفات عديدة لميكروب واحد بنخزنهم في وين في
+
+532
+00:41:13,950 --> 00:41:18,330
+الباكره وبنصير لما نشوفهم بنسترجع ونقارن إذا
+
+533
+00:41:18,330 --> 00:41:22,010
+أقول إن أنا مش قادر أسترهيب بنستبدلها بإيش ببرامج
+
+534
+00:41:22,010 --> 00:41:25,750
+حاسوب برامج حاسوب بندخله الذاكرة بدءًا من الحفاظ
+
+535
+00:41:25,750 --> 00:41:29,190
+اللحظة بندخلها بالحاسوب ولما بيجينا ميكروب يقولي
+
+536
+00:41:29,190 --> 00:41:33,170
+بندخله الذاكرة صفاته بقول your microbe is كده وكده 
+
+537
+00:41:33,170 --> 00:41:37,380
+وصل الصورة فعملية التعرف للميكروهيزم بتتم
+
+538
+00:41:37,380 --> 00:41:41,740
+بالاستثناء لكن بالأساس معظم العالمين على الـ
+
+539
+00:41:41,740 --> 00:41:45,260
+classification كلمة morphology اللي هو ده اعتماد
+
+540
+00:41:45,260 --> 00:41:47,980
+الشكل هو احنا بقولك البكتيريا very simple ياما
+
+541
+00:41:47,980 --> 00:41:52,480
+عصوية ياما كروية ياما حلزونية physiology
+
+542
+00:41:52,480 --> 00:41:56,320
+التغذية تبعتها أنا بترجع بشوية كيف تتغذى وكيف
+
+543
+00:41:56,320 --> 00:42:00,960
+تنمو التغذية تبعتها هل تستخدم الـ substrate X ولا
+
+544
+00:42:00,960 --> 00:42:06,140
+الـ substrate Y الأخرى الـ Genetics تبعتها بعض
+
+545
+00:42:06,140 --> 00:42:12,700
+الأحيان .. طبعًا هنا قلنا قبل شوية أنه هناك تغيير
+
+546
+00:42:12,700 --> 00:42:16,360
+مستمر في التصنيف تبع الميكروبات مستثمر في درجة أن
+
+547
+00:42:16,360 --> 00:42:21,360
+معارفنا بتزيد في السنوات الأخيرة علم الوراثة
+
+548
+00:42:21,360 --> 00:42:26,080
+الجزئية قفز قفزات نوعية وبالتالي بالفعل ساعدنا في
+
+549
+00:42:26,080 --> 00:42:29,060
+عملية اللي هو التصنيف مرة أخرى الـ ecology البيئة
+
+550
+00:42:29,060 --> 00:42:32,480
+تبعة الميكروبات under certain definite conditions
+
+551
+00:42:32,600 --> 00:42:38,080
+تحت ظروف اللي هو معين ثاني كمان هنا شغل ثانية the
+
+552
+00:42:38,080 --> 00:42:43,580
+law of their life activities ما النظام اللي يسيطر
+
+553
+00:42:43,580 --> 00:42:47,940
+أو يحكم تصرف اللي هو الـ micro organism and their
+
+554
+00:42:47,940 --> 00:42:51,520
+interaction with the human being كيف نتفاعل مع
+
+555
+00:42:51,520 --> 00:42:58,610
+البشر سلبًا، إيجابًا، فائدة، mutualism يعني اتفاد
+
+556
+00:42:58,610 --> 00:43:03,210
+منفعة ولا harmful relation هذا كلها ضمن الـ micro
+
+557
+00:43:03,210 --> 00:43:07,030
+environment طبعًا interaction with human beings
+
+558
+00:43:07,030 --> 00:43:10,270
+animates our plan as well as natural environment
+
+559
+00:43:10,270 --> 00:43:18,890
+أنتوا بتعرفوا أنه كتير من البيئات فسدت بسبب طمع
+
+560
+00:43:18,890 --> 00:43:24,520
+البشر خدوها قاعدة أينما وجد الماء على .. تاريخيًا
+
+561
+00:43:24,520 --> 00:43:28,560
+استوطن الناس .. استوطن الناس وبتعرف كل الحضارات
+
+562
+00:43:28,560 --> 00:43:34,100
+نجحت على الأودية والأنهار وأينما حل الإنسان واستوطن حل الفساد الشيء كان إيه؟ لإنه بيطلع البحر
+
+563
+00:43:34,100 --> 00:43:38,880
+.. قطع المية .. بيجيه المية جارية .. بيصيب
+
+564
+00:43:38,880 --> 00:43:41,520
+النفايات تبعته يحطها .. ماشي النفايات المية
+
+565
+00:43:41,520 --> 00:43:45,080
+الجارية هيخدها للتطبيق المعين .. لكن لإن حجم
+
+566
+00:43:45,080 --> 00:43:48,220
+النفايات بيزيد بنسميها زاد أو فاق عن قدرة الـ self
+
+567
+00:43:48,220 --> 00:43:54,160
+-purification ليها التنقية الذاتية، ليها ده بيسب
+
+568
+00:43:54,160 --> 00:43:57,080
+ب من الماء وبالتالي بيفسد، وبالتالي بيفسد فالـ
+
+569
+00:43:57,080 --> 00:44:01,200
+interaction ما بين الإنسان والـ environment قد
+
+570
+00:44:01,200 --> 00:44:06,440
+يكون واصل بينهم الـ micro organism يعني كتير من
+
+571
+00:44:09,460 --> 00:44:14,340
+البيئة استرجعناها بعد ما فسدت باستخدام micro
+
+572
+00:44:14,340 --> 00:44:19,060
+organism بنحط فيها micro organism خلطات معينة كي
+
+573
+00:44:19,060 --> 00:44:23,440
+بتسترجعنا البيئة، بتكسر المواد الضارة، بتشيل بعض
+
+574
+00:44:23,440 --> 00:44:29,700
+السموم من البيئة، وبالتالي بنرجع البيئة بشكل جيد
+
+575
+00:44:29,700 --> 00:44:35,420
+أنا قلت إنه كتير microbiology مهمة في حياتنا، مهمة
+
+576
+00:44:35,420 --> 00:44:38,160
+في الـ environment، مهمة في الطب، غير إنها بتسببنا
+
+577
+00:44:38,160 --> 00:44:43,560
+أمراض، علاجات، بنفلق منها، في الـ food الإنسان عرف
+
+578
+00:44:43,560 --> 00:44:48,280
+الميكروبات، خدوا بالكوا، عرف الميكروبات قبل العلم
+
+579
+00:44:48,280 --> 00:44:52,840
+الحديث المصريين القدامى لا عندهم microscope ولا
+
+580
+00:44:52,840 --> 00:44:56,800
+عندهم أي أجهزة من الأجهزة اللي .. اللي موجودة وبعد
+
+581
+00:44:56,800 --> 00:45:00,440
+ذلك صنعوا الخبز والخبز معروف أنه بتدخل بصناعته
+
+582
+00:45:00,440 --> 00:45:04,840
+مين الخميرة والخميرة micro organism ولحد وقت
+
+583
+00:45:04,840 --> 00:45:10,120
+قريب كانوا يعني مش .. مش أجدادكوا يعني آبائهم ..
+
+584
+00:45:10,120 --> 00:45:14,580
+آباء أجدادكوا كانوا العجين اللي بيعجنوه في البيت مش
+
+585
+00:45:14,580 --> 00:45:20,180
+بيشتروه خميرة بيخلوا جزء من عجين مبارك تمام؟ و
+
+586
+00:45:20,180 --> 00:45:25,960
+بيخلطوه مع عجين بكرة يعني بياخدوا الخميرة لمن؟ بنمو
+
+587
+00:45:25,960 --> 00:45:29,780
+الخميرة من عليه نفسه هذا بيصير مصدر الخميرة بيحطوه
+
+588
+00:45:29,780 --> 00:45:32,840
+لـ لـ لـ فهذا الكلام قبل ما يعرفوا حاجة من
+
+589
+00:45:32,840 --> 00:45:36,980
+الـ micro بيقولوا إن المصريين القدماء استخدموا
+
+590
+00:45:36,980 --> 00:45:42,500
+الخبز لمعفن تمام؟ لمداواة الجروح العفن هذا هو
+
+591
+00:45:42,500 --> 00:45:48,500
+penicillin يبقى الإنسان شعر بـ .. قيمة الـ micro
+
+592
+00:45:48,500 --> 00:45:52,020
+organism قبل حتى اللي هو الانتشار في العلم الحديث
+
+593
+00:45:52,020 --> 00:45:55,380
+عن الـ micro bio في الـ فوق الـ industry الـ bio
+
+594
+00:45:55,380 --> 00:45:58,840
+technology والـ research كلمة bio technology تسمع
+
+595
+00:45:58,840 --> 00:46:04,900
+إن فينا جسم هنا في الـ GMO اسمه bio technology عشان
+
+596
+00:46:04,900 --> 00:46:09,160
+أنا أقولكم جداش مهم الـ micro vat في الـ bio
+
+597
+00:46:09,160 --> 00:46:11,220
+technology فيه فرق في الـ bio technology اسمه
+
+598
+00:46:11,220 --> 00:46:15,140
+industrial microbiome industrial microbiome جزء من
+
+599
+00:46:15,140 --> 00:46:20,130
+مين؟ من الـ bio technology يقدر الـ industrial
+
+600
+00:46:20,130 --> 00:46:23,010
+microbiology investment اللي هو الاستثمار اللي
+
+601
+00:46:23,010 --> 00:46:27,330
+قاعد فيه في العالم ببلايين الدولارات سنوية فقطعهم
+
+602
+00:46:27,330 --> 00:46:31,450
+هفر ده طبعًا الـ research دايمًا بيقولوا في الطب the
+
+603
+00:46:31,450 --> 00:46:34,990
+research of today is the medicine of tomorrow
+
+604
+00:46:34,990 --> 00:46:38,570
+يعنيش معناها معناه يقولوا بيه البحث اليوم بيصير
+
+605
+00:46:38,570 --> 00:46:43,170
+الطب .. بيصير الطب للـ للـ ده نموذج على تصنيع
+
+606
+00:46:43,170 --> 00:46:47,850
+نتكلم عنه أنا قلت ممكن يوجهوا شوية لقصة طرفنا اللي
+
+607
+00:46:47,850 --> 00:46:52,170
+له حق بالطريق إن الإنسان شعر بـ الـ الـ
+
+608
+00:46:52,170 --> 00:46:57,430
+الميكروبات واستخدمها قبل ما يعرفها وبعدين بدأ ي
+
+609
+00:46:57,430 --> 00:47:01,490
+.. يتعرف عليها والآن في أنها طور بالفعل modern
+
+610
+00:47:01,490 --> 00:47:05,570
+جدًا ومتعامل مع .. إن احنا متعامل مع الميكروبات و
+
+611
+00:47:05,570 --> 00:47:12,550
+كأنها دواجن بننميها أو كأنها أبقار بنربيها بنتعامل
+
+612
+00:47:12,550 --> 00:47:17,430
+معها بطرق قياسية سهلة جدًا ورخيصة جدًا وبنستغرب
+
+613
+00:47:17,430 --> 00:47:21,910
+منها بشكل كام من
+
+614
+00:47:21,910 --> 00:47:26,650
+الناس اللي أثروا علم الـ microbiology Anthony van
+
+615
+00:47:26,650 --> 00:47:32,030
+Leeuwenhoek اللي اخترع اللي هو الـ microscope و
+
+616
+00:47:32,030 --> 00:47:37,770
+Louise Pastier اللي كان البسطر على اسمه تمام و
+
+617
+00:47:37,770 --> 00:47:43,770
+Robert Koch اللي عمل فرضية كوخ سموها Koch postulate
+
+618
+00:47:43,770 --> 00:47:47,410
+هتكلم عنها إن شاء الله لاحقًا بليستر اللي هو أتكلم
+
+619
+00:47:47,410 --> 00:47:50,950
+على الـ sterilization of this infection اللي هو
+
+620
+00:47:50,950 --> 00:47:56,310
+عامل تعقيمه في التطهير هذه طبعًا بعض الشخصيات اللي
+
+621
+00:47:56,310 --> 00:47:58,870
+أنا ذكرتها هذا Anthony van Leeuwenhoek وهي الـ
+
+622
+00:47:58,870 --> 00:48:06,650
+microscope تبعه اللي هو اللي عمله هذا
+
+623
+00:48:06,650 --> 00:48:11,820
+مين؟ هذا Edward Jenner Edward Jenner هذا اللي كان
+
+624
+00:48:11,820 --> 00:48:15,620
+مسؤول عن تطوير اللقاحات والـ vaccines اللي احنا
+
+626
+00:48:15,620 --> 00:48:19,020
+بنعرفها أو التقييمات اللي بنعرفها في العصر الحديث
+
+627
+00:48:19,020 --> 00:48:23,220
+طبعًا التقييمات كانت موقوفة سابقًا الصينيون عرفوها و
+
+628
+00:48:23,220 --> 00:48:28,540
+العرب عرفوها لكن ما عرفوهاش في طور المدن ولا في ال
+
+629
+00:48:28,540 --> 00:48:31,600
+experimental عرفوها في الـ pre اللي هي المرحلة
+
+630
+00:48:31,600 --> 00:48:33,860
+الأولى اللي قلنا لكم عملنا خبز واحنا مش عارفين
+
+631
+00:48:33,860 --> 00:48:39,780
+ليش الخبز صار هيك نفس الـ case كان فيه حاجة لطيفة في
+
+632
+00:48:39,780 --> 00:48:44,140
+السعودية والعراق في مرة اسمه Baghdad Sour قرحة
+
+633
+00:48:44,140 --> 00:48:47,760
+بغداد.. قرحة بغداد هنا فيها ريحة مولودة في في
+
+634
+00:48:47,760 --> 00:48:52,640
+فلسطين عبارة عن ذبابة بتعض الـ .. الـ .. الـ
+
+635
+00:48:52,640 --> 00:48:56,180
+السابق تنقله برازيت اسمه Leishmania هنا الـ
+
+636
+00:48:56,180 --> 00:49:00,480
+Leishmania مشهورة في اليمن والسعودية والعراق لما
+
+637
+00:49:00,480 --> 00:49:06,020
+تصيب الفتاة في الوجه مثلًا تعملها بجعة دائرية كويس
+
+638
+00:49:06,020 --> 00:49:11,450
+trademark علامة خلاص هذه بتشوه اللي هو الشكل فكانت
+
+639
+00:49:11,450 --> 00:49:16,390
+السيدات اللي ومات يروحوا على واحدة مصابة وواحد
+
+640
+00:49:16,390 --> 00:49:20,570
+مصاب ياخذوا منه ويحقنوا بناتهم في مناطق.. في
+
+641
+00:49:20,570 --> 00:49:25,670
+مناطق من الدسم غير.. يغير الوجهة فكان عمال يتجلى
+
+642
+00:49:25,670 --> 00:49:29,250
+انتصاب بيها مرة واحدة وابتدا الحفرة في المكان اللي
+
+643
+00:49:29,250 --> 00:49:33,730
+يبين عنه غير ظاهر لكن تضمن أنه ما يصيبش الوجهة وهذه
+
+644
+00:49:33,730 --> 00:49:37,520
+كانت برضه من اللي هو عملية الـ vaccination اللي
+
+645
+00:49:37,520 --> 00:49:43,540
+أُخذت بالـ Experience هذا
+
+646
+00:49:43,540 --> 00:49:48,860
+الـ A. Louis Pasteur تميز
+
+647
+00:49:48,860 --> 00:49:54,320
+عشان أميزكم بين العلماء
+
+648
+00:49:54,320 --> 00:50:01,840
+كان رأسه مزدحم بالأفكار بمعنى أنه كان يقترح وعنده
+
+649
+00:50:01,840 --> 00:50:06,460
+أفكار يقول له ألف فكرة في الشهر تصبح منها فكرة واحدة
+
+650
+00:50:06,460 --> 00:50:17,920
+من الألف بالعكس أزميله Robert Koch Robert
+
+651
+00:50:17,920 --> 00:50:25,400
+Koch كان hard-headed عنيد يعني لا يقف نق بسهولة
+
+652
+00:50:25,400 --> 00:50:30,300
+تقرا وراه حدّه بضل وجهه وراه لم يخلصها وكان من نتائج
+
+653
+00:50:30,300 --> 00:50:33,500
+أعماله كانت فرضية Koch اللي هتطلقنا إن شاء الله
+
+654
+00:50:33,500 --> 00:50:37,060
+المحاضرة اللي جاية وهي فرضية رائعة جدًّا من خلالها
+
+655
+00:50:37,060 --> 00:50:40,800
+أثبت أن هذا الميكروب هو المسبب لهذا المرض أنا
+
+656
+00:50:40,800 --> 00:50:43,920
+أعتقد مرض عليه ممكن فيه حاجة أو شيء فهنطلق لها إن
+
+657
+00:50:43,920 --> 00:50:52,540
+شاء الله في
+
+658
+00:50:52,540 --> 00:50:57,040
+عالم اسمه Dimitry Ivanovsky هو عالم روسي هو اللي
+
+659
+00:50:57,040 --> 00:51:01,300
+اكتشف الفيروسات واكتشاف الفيروسات كان بطريقة يعني
+
+660
+00:51:01,300 --> 00:51:05,040
+فيها نوع من المصادفة أنه كان بيحاول أن يـ filter
+
+661
+00:51:05,480 --> 00:51:11,180
+العامل المسبب لمرض تبرقش الدخان، الدخان اللي هو الـ
+
+662
+00:51:11,180 --> 00:51:16,020
+tobacco، tobacco فكان
+
+663
+00:51:16,020 --> 00:51:19,400
+يميل عبر المرشحات البكتيرية ويأخذ السائل ويروشه
+
+664
+00:51:19,400 --> 00:51:22,500
+على النباتي، عوض النباتي الصعب فجال إن العامل
+
+665
+00:51:22,500 --> 00:51:28,360
+المسبب لتبرقش أوراق الـ tobacco هو filterable،
+
+666
+00:51:28,360 --> 00:51:31,400
+filterable يعني قابل.. يعني يدخل من.. يعني أنا
+
+667
+00:51:31,400 --> 00:51:35,210
+حاجة وزرمة وبعدين اهيب هو أن هو المرة جاي إن شاء
+
+668
+00:51:35,210 --> 00:51:38,350
+الله نكمل مقدماتنا ونبدأ بمحاضرة أول إن شاء الله
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/m85QUrbJGqM_postprocess.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/m85QUrbJGqM_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..035a850b067e7381ce952cd16c8af88da7f8d916
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/m85QUrbJGqM_postprocess.srt
@@ -0,0 +1,2672 @@
+1
+00:00:20,870 --> 00:00:23,330
+بسم الله الرحمن الرحيم الحمد لله ربنا عليه الصلاة
+
+2
+00:00:23,330 --> 00:00:26,330
+والسلام وعلى سيدنا محمد وعلى آله وصحبه ومن ساعة
+
+3
+00:00:26,330 --> 00:00:30,330
+دربيه للمدين نرحب بكم في بداية هذا الفصل الدراسي
+
+4
+00:00:30,330 --> 00:00:36,190
+في مساق الأحياء الدقيقة الطبية الأساسية أو كما
+
+5
+00:00:36,190 --> 00:00:38,550
+تدعى باللغة الميزية essential medical microbiology
+
+6
+00:00:38,550 --> 00:00:43,530
+مش هندرس اليوم microbiology لكن هحنحاول قدر
+
+7
+00:00:43,530 --> 00:00:47,850
+الإمكان نسوق لهذا المساق نقولكوا أن هو هذا مساق
+
+8
+00:00:47,850 --> 00:00:53,120
+رائعجيد ممتاز أساسي وضروري لمهنة العلم الطبية
+
+9
+00:00:53,120 --> 00:00:56,760
+المخبرية يعني مش من المساقات الثانوية وانما من
+
+10
+00:00:56,760 --> 00:01:01,700
+المساقات الأساسية حتى نبدأ بالتسويق لهذا المساق
+
+11
+00:01:01,700 --> 00:01:04,540
+بدنا نبدأ من الكلمة الأخيرة microbiology
+
+12
+00:01:04,540 --> 00:01:07,640
+microbiology كلكم تعرفوا التعريف تبعها انه هي
+
+13
+00:01:07,640 --> 00:01:11,360
+دراسة الأحياء فكرة عام micro بتكلم عن دراسة
+
+14
+00:01:11,360 --> 00:01:15,140
+الأحياء الدقيقة يعني لو دينا نعرف تعريف بسيط بنقول
+
+15
+00:01:15,140 --> 00:01:19,380
+the study of microorganismال key word في هذا
+
+16
+00:01:19,380 --> 00:01:23,040
+الموضوع كلمة micro organism what is a micro
+
+17
+00:01:23,040 --> 00:01:27,720
+organism micro organism is a small living micro
+
+18
+00:01:27,720 --> 00:01:30,920
+organism that cannot be seen by the naked او اللي
+
+19
+00:01:30,920 --> 00:01:35,740
+بيسميها unaided eye يعني العين المجردة او العين
+
+20
+00:01:35,740 --> 00:01:39,520
+اللي مالهاش مساعدة مالهاش نضارات مالهاش microscope
+
+21
+00:01:39,520 --> 00:01:45,660
+لها اذا هذا هو ال micro biome لكن حتى التعريف هذا
+
+22
+00:01:45,660 --> 00:01:49,550
+اللي احنا عرفنا للأسفهنضطر نعمل justification أو
+
+23
+00:01:49,550 --> 00:01:56,610
+تبريرات لماذا ضمنا دودة طولها 7 متر ضمن الميكرو
+
+24
+00:01:56,610 --> 00:02:00,130
+بيولوجيا في علم البراسيتولوجيا ولماذا دودة
+
+25
+00:02:00,130 --> 00:02:04,030
+الاسكاريس اللي طولها 30 سانتي برضه مدرسة تحت
+
+26
+00:02:04,030 --> 00:02:06,890
+المايكرو هل .. هل بدنا مايكروسكوب حتى نشوف دودة 30
+
+27
+00:02:06,890 --> 00:02:13,010
+سانتي مصطرة؟ بدناش مايكروسكوبone stage of the life
+
+28
+00:02:13,010 --> 00:02:16,190
+cycle of this parasite is microscopic يعني مرحل
+
+29
+00:02:16,190 --> 00:02:23,010
+مرحل حياتها بتكون مجهرية برضه مش مبرر احنا كبشر
+
+30
+00:02:23,010 --> 00:02:27,490
+مرحل من مرحل حياتنا بنكون مجهرين برضه صح ولا لأ
+
+31
+00:02:27,490 --> 00:02:34,630
+التبرير التاني ان they cause disease يعني بسببه
+
+32
+00:02:34,630 --> 00:02:37,850
+أمراض زي البكتيريا اللي هي بالفعل بينطبق عليها
+
+33
+00:02:37,850 --> 00:02:42,310
+التعريفبنعمل كمان justification الفيروسات نحن كنا
+
+34
+00:02:42,310 --> 00:02:46,990
+نتكلم عن living microorganisms هل الفيروسات living
+
+35
+00:02:46,990 --> 00:02:53,250
+microorganisms؟ هل هي أصلا ينطبق عليها organism؟
+
+36
+00:02:53,250 --> 00:02:57,370
+هل هي خلوية؟ يعني إيه الخلية؟ الفيروسات؟ مالهاش
+
+37
+00:02:57,370 --> 00:03:01,950
+وبالتالي انطبق عليها جزء الأول من التعريف اللي هو
+
+38
+00:03:01,950 --> 00:03:06,470
+كلمة micro لأنها small لكن لم ينطبق عليها biology
+
+39
+00:03:06,910 --> 00:03:09,730
+وما ريح نجرسها في ال microbiology justification
+
+40
+00:03:09,730 --> 00:03:17,230
+تبرير ان الفيروسات تمتلك صفة من صفات الخلايا الحية
+
+41
+00:03:17,230 --> 00:03:23,430
+اللي و هي التكاثر عن طريق انها تستخدم host cell او
+
+42
+00:03:23,430 --> 00:03:28,350
+يعني خلايا عائل معين نبات او حيوان او حتى بعض
+
+43
+00:03:28,350 --> 00:03:32,110
+الفيروسات تتطفل على ال bacteria بيسموها
+
+44
+00:03:32,110 --> 00:03:35,550
+bacteriophytes الفطريات
+
+45
+00:03:36,710 --> 00:03:41,410
+كلكم أكلتم مشروم المشروم فطرية البعض منكم أصابوا
+
+46
+00:03:41,410 --> 00:03:45,190
+بين أصابع أرجليه التهابات فطرية اللي بتطلع رايحة
+
+47
+00:03:45,190 --> 00:03:49,470
+كريهة بعض الأحيان عند لبس الأحدى لفترات طويلة اللي
+
+48
+00:03:49,470 --> 00:03:54,290
+بيسموها قدم رياضية قدم رياضية ويعني بمان أخر في
+
+49
+00:03:54,290 --> 00:03:58,910
+microscopic وفي macroscopic لكن ال sports تبعتها
+
+50
+00:03:58,910 --> 00:04:03,130
+كلها microscopic سواء كانت الفطريات الكبيرة أو
+
+51
+00:04:03,130 --> 00:04:07,090
+الفطريات ال microscopic ال sports تبعتهاmicroscope
+
+52
+00:04:07,090 --> 00:04:11,630
+إذا متفقين على ال microbiology على تعريفها وعلى
+
+53
+00:04:11,630 --> 00:04:15,710
+التبريرات لماذا ضمنها بعض المخلوقات اللي هي لا
+
+54
+00:04:15,710 --> 00:04:20,370
+ينطبق عليها التعريف ضمن هذا التعريف نيجي لكلمة
+
+55
+00:04:20,370 --> 00:04:25,250
+medical ليش خصصنا medical شوفوا أنا في ال
+
+56
+00:04:25,250 --> 00:04:29,310
+microbiology أنا أعمل في مجال ال food microbiology
+
+57
+00:04:29,310 --> 00:04:35,130
+كلمة food food testing services يعني في عمليةفحص
+
+58
+00:04:35,130 --> 00:04:38,570
+الأغذية، فحص سلامة الأغذية في زمايا إيه اللي
+
+59
+00:04:38,570 --> 00:04:43,110
+بيشتغلوا في مجال تصنيع الأغذية، microbiology إيه
+
+60
+00:04:43,110 --> 00:04:45,970
+في مجال ال environmental microbiology، البيئة،
+
+61
+00:04:45,970 --> 00:04:48,470
+بروح على البحر باخد عينات و بعملها testing، هذا
+
+62
+00:04:48,470 --> 00:04:53,230
+بيئة ولا لأ، برد ناخد من الهوا عينات و بنتحصها،
+
+63
+00:04:53,230 --> 00:04:57,430
+هذا environmental microbiology قبل قدر السنوات
+
+64
+00:04:57,430 --> 00:05:01,670
+أنتجنا بكتيريا لمكافحة البعوض، كنا .. يعني نمّي
+
+65
+00:05:01,670 --> 00:05:05,160
+البكتيريا، بدنا البكتيريا نفسهاوبنرشها على
+
+66
+00:05:05,160 --> 00:05:10,660
+المستنقعات يرقات البعوض بتاكلها و بتموت هذا اسمه
+
+67
+00:05:10,660 --> 00:05:14,500
+Environmental و Industrial في نفس اللوجب ليش؟ احنا
+
+68
+00:05:14,500 --> 00:05:18,380
+قضينا على بعوض او مش قضينا بالاحرار خففنا لان كلمة
+
+69
+00:05:18,380 --> 00:05:22,720
+قضينا موضوع صعب خففنا من البعوض بهذا التقنية إذا
+
+70
+00:05:22,720 --> 00:05:26,240
+ال microbiology في مجالات متنوعة حمض الليمون اللي
+
+71
+00:05:26,240 --> 00:05:29,940
+بيستخدموه في المطبخ هذا تسعة و تسعين و تسعة من
+
+72
+00:05:29,940 --> 00:05:38,130
+عشرة من في الأسواق هو منتوج منالفطريات معظم
+
+73
+00:05:38,130 --> 00:05:42,290
+ال antibiotics اللى بتتناولوها فى العلاجات هى منتج
+
+74
+00:05:42,290 --> 00:05:47,270
+من منتجات ال micro organism يبقى مقدرش احصل ال
+
+75
+00:05:47,270 --> 00:05:51,470
+microbiology فى ال medical microbiology ولا بقدر
+
+76
+00:05:51,470 --> 00:05:55,770
+أسيب ال microbiology فاضى مفتوحة فاضى فاضى مظبوط
+
+77
+00:05:55,770 --> 00:05:59,510
+وبالتالي حددت انا في industrial اقولكوا فى ال food
+
+78
+00:05:59,510 --> 00:06:02,960
+microbiology صح فى انwater microbiology مع ان ال
+
+79
+00:06:02,960 --> 00:06:06,520
+water كان من مين ال food في ال food microbiology
+
+80
+00:06:06,520 --> 00:06:10,440
+صرنا نصنف الأغضية و نعطيها كاسم microbiology دلوقت
+
+81
+00:06:10,440 --> 00:06:15,780
+على توسع العلوم او علم الميكروبيري و تداخله في كل
+
+82
+00:06:15,780 --> 00:06:20,220
+المجارات هعطيكم مجارة رائعة جدا قبل سنتين دخلنا
+
+83
+00:06:20,220 --> 00:06:25,360
+تعرف البطون؟ لمين بيشتغلون بالبطون في الجامعة؟
+
+84
+00:06:25,360 --> 00:06:28,360
+لندسة المدنية دلوقت هم اللي بيشتغلوا بالبطون احنا
+
+85
+00:06:28,360 --> 00:06:33,170
+مش دخلنا فيه بالبطونطيورة من قسم التحيات الطبية
+
+86
+00:06:33,170 --> 00:06:38,270
+لأن هي مؤيدة و بتدرس ماليستير في القردن عملنا
+
+87
+00:06:38,270 --> 00:06:43,690
+دراسة على استخدام البكتيريا في تقوية الإسمنت
+
+88
+00:06:43,690 --> 00:06:47,170
+المسلح يعني أضافنا بكتيريا من نوع معين و أضافنا
+
+89
+00:06:47,170 --> 00:06:52,330
+بعض ال chemicals نعمل reaction نخلي البطون يزداد
+
+90
+00:06:52,330 --> 00:06:55,510
+قدرته أو فيسموه compressive strength test قوة
+
+91
+00:06:55,510 --> 00:07:03,930
+تحمله إلى 30% إضافيوقللنا من قدرة امتصاصه الى اكثر
+
+92
+00:07:03,930 --> 00:07:09,890
+من 30% ايضا بمعنى اخر صار ان أسمنت مقاوم للرطوبة و
+
+93
+00:07:09,890 --> 00:07:14,670
+أسمنت أكتر صلابة و أكتر قدرة على التحمل كل هذا ضمن
+
+94
+00:07:14,670 --> 00:07:22,570
+من؟ لكن للأسف جدش الصورة اللى وصرتها جميلة السيئين
+
+95
+00:07:23,220 --> 00:07:27,920
+من الميكروبات هم اللي أكتر سمعة يعني هم الأكتر
+
+96
+00:07:27,920 --> 00:07:32,340
+شغرة في العالم وبالتالي لما بنقول ميكروبات بيخطروا
+
+97
+00:07:32,340 --> 00:07:36,220
+على بالك وليش جيد وليش سيء سيء لأنه ال medical
+
+98
+00:07:36,220 --> 00:07:42,560
+microbi الانسان يتذكر فقط من الميكروبات اللي سببله
+
+99
+00:07:42,560 --> 00:07:47,180
+أداء لكن تلك اللي تسبب أداء لا تشكل واحد إلى اتنين
+
+100
+00:07:47,180 --> 00:07:52,900
+في المية من مجموعة الميكروبات إذا من 98% إلى 99%من
+
+101
+00:07:52,900 --> 00:07:58,480
+الميكروبات ذو فائدة و ذو فائدة عظيمة تخيلوا ..
+
+102
+00:07:58,480 --> 00:08:05,420
+تخيلوا لو أن أشعة ما ضربت كوكب الأرض و قتلت كل
+
+103
+00:08:05,420 --> 00:08:09,220
+البكتيريا و المخلوقات الميكرو أورجينزية ما الذي
+
+104
+00:08:09,220 --> 00:08:12,920
+سيحدث .. عدا أني هبطل أدرس micro biology أنا بقى
+
+105
+00:08:12,920 --> 00:08:16,660
+مدمرت الميكروبات خلاص ما الذي سيحصل لكوكب الأرض
+
+106
+00:08:16,660 --> 00:08:24,420
+بعد عدة سنوات كل المخلوقات هتموتإذا بنقدر نقول إنه
+
+107
+00:08:24,420 --> 00:08:28,060
+هدف إحنا بنحن نتكلم عن ناحية نظرية ليش استنتجنا
+
+108
+00:08:28,060 --> 00:08:33,840
+هذا الاستنتاج؟ لأنه الطاقة الكامنة فينا احنا ..
+
+109
+00:08:33,840 --> 00:08:39,020
+احنا كانت طاقة كامنة خزّلناها بشكل غداء تقزّن بشكل
+
+110
+00:08:39,020 --> 00:08:42,260
+carbohydrate و protein ودهون في دسم الإنسان
+
+111
+00:08:42,260 --> 00:08:45,920
+بالإضافة إلى عناصر زي ال carbon و ال nitrogen و ال
+
+112
+00:08:45,920 --> 00:08:48,500
+phosphorus و ال oxygen كل هذه العناصر اللي مختزنة
+
+113
+00:08:48,500 --> 00:08:53,180
+في أجزامنا تخيلوا أنه هذه العناصرلم تدور في
+
+114
+00:08:53,180 --> 00:08:56,560
+الطبيعة لم تدور يعني ما انا اخر ماموتناش احنا
+
+115
+00:08:56,560 --> 00:08:59,460
+ماتحللناش مش اقصد احنا بس احنا على مستوانا
+
+116
+00:08:59,460 --> 00:09:03,420
+المخلوقات الأخرى جميعا النباتات طب مين اللي بيجوم
+
+117
+00:09:03,420 --> 00:09:07,320
+بعمل التحلل هذه ال micro organisms إذا ال micro
+
+118
+00:09:07,320 --> 00:09:12,320
+organisms بتجوم بدور رائع جدا في الطبيعة وبالتالي
+
+119
+00:09:12,320 --> 00:09:16,560
+لا يجب علينا أن ننسى كل هذا الدور الرائع و اللخص
+
+120
+00:09:16,560 --> 00:09:20,360
+حنا في مين في ال medical microbial ننتقل أقل مين
+
+121
+00:09:20,360 --> 00:09:25,560
+licensureessential يعني بيسموها أساسي لأ مش أساسي
+
+122
+00:09:25,560 --> 00:09:30,560
+ضروري فهل يعني .. هل معناها انه هن .. هن .. هنفصل
+
+123
+00:09:30,560 --> 00:09:34,460
+كثيرا في ال .. في المصاد هذا ولا هناخد المعلومات
+
+124
+00:09:34,460 --> 00:09:37,480
+الأساسية غالبا هناخد المعلومات الأساسية الطبية
+
+125
+00:09:37,480 --> 00:09:42,740
+بتوسع و ليس بعمق بمعنى هناخد معلومات كبيرة كثيرة
+
+126
+00:09:42,740 --> 00:09:49,640
+عفوا لكن مش عمق انا حاولت اقولكوا ان ال
+
+127
+00:09:49,640 --> 00:09:54,750
+microbiology microbiologyمرتبطة كثيرا بتعليف كلمة
+
+128
+00:09:54,750 --> 00:09:58,090
+مين micro organisms وحاولت أقولكوا إيش يعني
+
+129
+00:09:58,090 --> 00:10:02,030
+medical microbiology لكن بنركز عليها الآن بتركيز
+
+130
+00:10:02,030 --> 00:10:09,810
+أعلى ال medical microbes خلينا ننهج للموضوع احنا
+
+131
+00:10:09,810 --> 00:10:17,750
+لو يعني كنا نايمين وصحينا من النوم الساعة مثلا
+
+132
+00:10:17,750 --> 00:10:23,830
+تلاتة الفجرهنحط أصبعنا قدامنا خاصة في ضلقة الكهربا
+
+133
+00:10:23,830 --> 00:10:30,590
+مش هنشوف طاق خلينا صحيه لساعة عشر الصبح تمام؟ عشر
+
+134
+00:10:30,590 --> 00:10:34,870
+الصبح كيف يكون الجو؟ زي ما احنا الآن، نهار، بين
+
+135
+00:10:34,870 --> 00:10:39,090
+الأربعة، الأربعة الفجر إلى عشر الصبح، هل درجات
+
+136
+00:10:39,090 --> 00:10:45,420
+الظلمة و البنارة كانت واحدة؟ صرنا نشوف، لكنمش
+
+137
+00:10:45,420 --> 00:10:49,780
+بدرجة واضحة بعدين تدرجيا و بعدين سنشوف بدرجة واضحة
+
+138
+00:10:49,780 --> 00:10:54,460
+إذا ماكانش في عندي ظلام دانس أو نور ساطا بين
+
+139
+00:10:54,460 --> 00:10:57,640
+الفترين فقط لأ كان في عندي فترات مافيش فيها وضع
+
+140
+00:10:57,640 --> 00:11:02,960
+ترقية وهذا الكلام بدنا نحاول إن المفهوم هنا نغرصه
+
+141
+00:11:02,960 --> 00:11:08,800
+فيكوا لما نتكلم عن ال medical microbes ميكروب ممرض
+
+142
+00:11:08,800 --> 00:11:13,760
+و ميكروب غير ممرض نحط ذلك في الظلام ال dance
+
+143
+00:11:13,760 --> 00:11:18,940
+الممرضوالغير ممرض في النور الصافية لكن فيما بينهما
+
+144
+00:11:18,940 --> 00:11:23,900
+في ميكروبات قد يسبب أمراض وميكروبات أخرى أو نفس
+
+145
+00:11:23,900 --> 00:11:27,460
+الميكروب قد لا يسبب أمراض بمعنى لو رشينا عليكم إن
+
+146
+00:11:27,460 --> 00:11:31,660
+الميكروب هذا الآن جزء أمام البعض منكم ممكن يقول
+
+147
+00:11:31,660 --> 00:11:36,840
+البعض الأخر مانمرضش يجب ليش نفهم إن الممرض هو
+
+148
+00:11:36,840 --> 00:11:42,240
+absolute يعني مصطلح مطلق وإنما مصطلح relative في
+
+149
+00:11:42,240 --> 00:11:47,220
+عوامل أخرى تتحكم فيهفي دائما مايكروبات معروفة عنها
+
+150
+00:11:47,220 --> 00:11:51,880
+انها pathogenic و مايكروبات اخرى non pathogenic
+
+151
+00:11:51,880 --> 00:11:54,300
+pathogenic يعني ممرضة و non pathogenic يعني غير
+
+152
+00:11:54,300 --> 00:11:58,200
+ممرضة في ال medical microbiology غالبا هيتطرق
+
+153
+00:11:58,200 --> 00:12:02,720
+للنوعين، للممرضة و للغير ممرضة، لأنه بهمنا نعمل
+
+154
+00:12:02,720 --> 00:12:05,820
+differentiation، اللي هو عملية تمييز بين
+
+155
+00:12:08,580 --> 00:12:11,680
+أحد النقاط المهمة جدا في ال medical microbial اللي
+
+156
+00:12:11,680 --> 00:12:14,140
+دراستوها في التنمية العالمية و دراستوها في الأحياء
+
+157
+00:12:14,140 --> 00:12:18,080
+تبعت مستوى أول هو الفروقات بين ال prokaryotes و ال
+
+158
+00:12:18,080 --> 00:12:22,740
+eukaryotes طبعا هذكركم الفرق الرئيس عشان أنا بدي
+
+159
+00:12:22,740 --> 00:12:25,280
+أخد منه بس من جدول هذا بدي أخد منه معلومة أو
+
+160
+00:12:25,280 --> 00:12:29,860
+معلومتان عشان أوصلكم فكرة أساسية أن كيف بنستخدم أو
+
+161
+00:12:29,860 --> 00:12:33,240
+بنوظف الفروقات بين ال prokaryotes و ال eukaryotes
+
+162
+00:12:33,240 --> 00:12:38,620
+حقيقية النوة يعني في إيها غشاء نويةوبداية النوية
+
+163
+00:12:38,620 --> 00:12:43,800
+أنا مالهاش غشاء النوية هذا التعريف الأساسي لكن فيه
+
+164
+00:12:43,800 --> 00:12:47,380
+فروقات أخرى كثيرة شايفين الدول كبيرة أنا بدي أاخد
+
+165
+00:12:47,380 --> 00:12:52,460
+فرق أساسي أخر و أنطلق منه تلاف الريبوزوم ما هي
+
+166
+00:12:52,460 --> 00:12:57,420
+وظيفة الريبوزوم اسمها ال protein فاكرين الكورة
+
+167
+00:12:57,420 --> 00:13:00,660
+الكبيرة و الكورة الصغيرة اللي هي ال large subunit
+
+168
+00:13:00,660 --> 00:13:05,280
+و ال small subunit في الإيوكاريوت الريبوزوم هذا
+
+169
+00:13:05,280 --> 00:13:12,350
+القطعتينبنسميهم ATS AT يعني تمانين انا مش ارسم
+
+170
+00:13:12,350 --> 00:13:20,390
+تمانين اس نعم بالكود
+
+171
+00:13:20,390 --> 00:13:25,930
+سامحوني انا في الرسم يعني كتير قوي فهتنبهروا من
+
+172
+00:13:25,930 --> 00:13:30,770
+الرسمات اللي برسمها هاي أول دائرة اعتقد هذه أحسن
+
+173
+00:13:30,770 --> 00:13:31,670
+دائرة اللي برسمها انا
+
+174
+00:13:36,630 --> 00:13:42,910
+80S كل ريبوزون هذا لو حطيناه في جهاز ترسيب هيترصب
+
+175
+00:13:42,910 --> 00:13:49,550
+على سرعة 80 سرعة 80 لو فصلناهم عن بعض قطعة قطعة
+
+176
+00:13:49,550 --> 00:13:53,410
+هذا الكبيرة وهذا الصغيرة وحطينا الكبيرة في جهاز
+
+177
+00:13:53,410 --> 00:13:58,230
+الترسيب هيترصب على 50 از 2 وهذا هيترصب على 40 محدش
+
+178
+00:13:58,230 --> 00:14:01,830
+مولد يقول 60 40 100 لا مش جمال العملية العملية مش
+
+179
+00:14:01,830 --> 00:14:08,050
+جمال العملية سرعة ترسيبكله على بعضه ترسب عن تمانين
+
+180
+00:14:08,050 --> 00:14:12,890
+لما فصلناهم على ستين هذا على أربعين بينما الآخر
+
+181
+00:14:12,890 --> 00:14:15,930
+اللي هو اللي تبقى ال bacterial بروكاريوتز اسمه
+
+182
+00:14:15,930 --> 00:14:20,370
+سبعين أس يعني كله ترسب على كتير على سبعين الكبيرة
+
+183
+00:14:20,370 --> 00:14:24,110
+على خمسين و الصغيرة على تلاتين جمعناهم مع بعض أول
+
+184
+00:14:24,110 --> 00:14:27,630
+ما جمعناهم مش هذه المعلومة اللي هطلبها الوحيد اللي
+
+185
+00:14:27,630 --> 00:14:32,750
+هتحفظوها من المحاضرة هذه سبعين أس و تمانين أس
+
+186
+00:14:32,750 --> 00:14:37,780
+التمانين ستين اربعينوالخمسين تلاتين ثانية ليش أنا
+
+187
+00:14:37,780 --> 00:14:43,160
+بطلب المعلومة دلوقت أفهى لأنه إذا أحنا عرفنا
+
+188
+00:14:43,160 --> 00:14:49,020
+المعلومات وحفظناها في أدمغتنا ولم نستخدمها فهي
+
+189
+00:14:49,020 --> 00:14:53,520
+عديمة نفاية لا علم إلا ما ينفع لازم العلم يكون
+
+190
+00:14:53,520 --> 00:14:59,400
+نافع لازم نستفيد منه بناخد antibiotic لما بصيبنا
+
+191
+00:14:59,400 --> 00:15:03,890
+التهاب احنا ال antibioticبيقضي على ال protein
+
+192
+00:15:03,890 --> 00:15:06,550
+synthesis أو بيوقف ال protein synthesis في الخلايا
+
+193
+00:15:06,550 --> 00:15:12,110
+لماذا يوقف ال protein synthesis في البكتيريا ولا
+
+194
+00:15:12,110 --> 00:15:17,610
+يوقف ال protein synthesis في خلاينا لأنه بيستهدف
+
+195
+00:15:17,610 --> 00:15:23,590
+ال 30S ribosomal subunit ال 30S في من موجود هذه؟
+
+196
+00:15:23,590 --> 00:15:27,510
+في البكتيريا ال بروكاريوتز طب هل فينا احنا احنا
+
+197
+00:15:27,510 --> 00:15:31,990
+ايه بروكاريوتز؟ هل فينا 30S؟ مش اللي احنا احنا 40S
+
+198
+00:15:33,350 --> 00:15:36,330
+استهدفنا .. استهدفنا او ال .. ال .. ال antibiotic
+
+199
+00:15:36,330 --> 00:15:41,350
+استهدف ال 30S ما استهدفش مين؟ طب ليش بيستهدف ال
+
+200
+00:15:41,350 --> 00:15:43,990
+30S بيستهدفش ال 40S؟ زي ما انتوا جيتوا على الغرفة
+
+201
+00:15:43,990 --> 00:15:48,250
+هذه يا بنات خاص نص ماجيتوش لغرف تانية للمحاضرة هذه
+
+202
+00:15:48,250 --> 00:15:52,810
+لانكو معاك ورقة ومسمحوا لكم الدخول في هذا الوقت
+
+203
+00:15:52,810 --> 00:15:57,250
+صح؟ ال binding بين المركبات وبين الخلايا بيكون
+
+204
+00:15:57,250 --> 00:16:00,650
+specificفبيكون فيه receptor لل antibiotic على ال
+
+205
+00:16:00,650 --> 00:16:05,590
+30S ومافيش receptor على ال 40S إذا هي استفادتنا من
+
+206
+00:16:05,590 --> 00:16:08,690
+معلومة اسمها فروقات ما بين البروكاريوت
+
+207
+00:16:08,690 --> 00:16:13,850
+والإيوكاريوت فارق آخر إحنا خلايانا كإيوكاريوت
+
+208
+00:16:13,850 --> 00:16:17,050
+مالناش cell wall صح؟ ولا نا cell wall؟ لو إلنا
+
+209
+00:16:17,050 --> 00:16:21,070
+cell wall كنا سنة زي الطاولة، خشب، مظبوط؟ بس
+
+210
+00:16:21,070 --> 00:16:23,590
+مالناش cell wall البكتيريا إلها cell wall
+
+211
+00:16:23,590 --> 00:16:29,210
+البنسالين المشهور عالميابيقتل البكتيريا بإنه
+
+212
+00:16:29,210 --> 00:16:34,010
+بيستهدف ال cell wall synthesis بيوقف تصنيع ال cell
+
+213
+00:16:34,010 --> 00:16:37,470
+wall يبقى بيقتل البكتيريا بيقتلناش احنا لإن احنا
+
+214
+00:16:37,470 --> 00:16:41,110
+أصلا ماناش cell wall إذا المعرفة دي و الفروق بين
+
+215
+00:16:41,110 --> 00:16:44,890
+ال eukaryote و ال prokaryote مهمة جدا لتطبيقات
+
+216
+00:16:44,890 --> 00:16:50,710
+مستقبلية كمان فرق تالت و مهم جدا اللي هو ال
+
+217
+00:16:50,710 --> 00:16:56,030
+chromosome أكام كرموزوم عند البشريعني 23 ضرب اتنين
+
+218
+00:16:56,030 --> 00:17:01,850
+اظن ان هما ازواج لكن في البكتيريا it is only one
+
+219
+00:17:01,850 --> 00:17:06,790
+single chromosome و هذا ال chromosome بيقول
+
+220
+00:17:06,790 --> 00:17:12,330
+circular يعني مالهوش طرف بينما كرموزوماتنا احنا
+
+221
+00:17:12,330 --> 00:17:17,410
+linear خطية زي الجلم الجلم له كام طرف؟ طرفين بس
+
+222
+00:17:17,410 --> 00:17:22,610
+الدائرة مالهاش ولا طرف ايش ميزة اتنين و واحد؟ هاي
+
+223
+00:17:22,610 --> 00:17:28,610
+كرموزوموهي المقابل إله على الجهة التانية نفترض إنه
+
+224
+00:17:28,610 --> 00:17:33,670
+في جين نفس الجين هيكون وين؟ في الجهة التانية هذا
+
+225
+00:17:33,670 --> 00:17:38,450
+الجين صار فيه خلل طفرة mutation الجين التاني ال
+
+226
+00:17:38,450 --> 00:17:43,610
+expression تبعه ما زال قادر على إنتاج نفس المنتج
+
+227
+00:17:43,610 --> 00:17:47,910
+وبالتالي لن نشعر إنه أصلا صار فيه خلل في هذا الجين
+
+228
+00:17:47,910 --> 00:17:52,010
+لإنه فيه جين بديل بينتج نفس الإنتاج يبقى الطفرة في
+
+229
+00:17:52,010 --> 00:17:56,820
+هذه الحالة لم تظهر نتائجهالكن بالبكتيريا it is
+
+230
+00:17:56,820 --> 00:18:02,240
+only one single chromosome وبالتالي أي تثرة هتظهر
+
+231
+00:18:02,240 --> 00:18:07,260
+و تثورة هتكتر نقطة مهمة it is easy to make
+
+232
+00:18:07,260 --> 00:18:12,200
+mutations in bacteria و بعض الأحيان احنا بنلجأ
+
+233
+00:18:12,200 --> 00:18:17,540
+لعمل mutation في البكتيريا لتحسين إنتاج منتج معين
+
+234
+00:18:17,540 --> 00:18:23,740
+مثلافي دراستنا أيام ال الستير كان فينا كطلاب نحاول
+
+235
+00:18:23,740 --> 00:18:27,560
+ننتج إنزين اسمه Alpha Amylase و الله عزلنا بكتيريا
+
+236
+00:18:27,560 --> 00:18:33,200
+و تمام اسمها Bacillus و ورايناها الدكتورة اللي
+
+237
+00:18:33,200 --> 00:18:37,700
+بتشرف علينا جالت كويسة بس إنتاجها قليل بدنا نحسن
+
+238
+00:18:37,700 --> 00:18:41,280
+إنتاجها طب كيف نحسن إنتاجها؟ جاءت يعملولها طفرات،
+
+239
+00:18:41,280 --> 00:18:46,420
+يعملولها طفرات انتوا يعني خربوها فاخدنا البكتيريا
+
+240
+00:18:46,420 --> 00:18:50,990
+و عرضناها لل ultra violet lightال ultraviolet
+
+241
+00:18:50,990 --> 00:18:54,890
+light تعمل mutations لفترات معينة زمانية trial and
+
+242
+00:18:54,890 --> 00:18:59,810
+error و في الآخر حصلنا على سلالة بتنتج alpha
+
+243
+00:18:59,810 --> 00:19:03,850
+amylase بكميات ضخمة بدون حساب طبعا الطفرة بالنسبة
+
+244
+00:19:03,850 --> 00:19:07,630
+لإن احنا نبلي إشلان كويسة إن احنا كبشر من وجهة
+
+245
+00:19:07,630 --> 00:19:11,190
+نظرنا احنا من وجهة نظر البكتيريا سيئة لأنها فقدت
+
+246
+00:19:11,190 --> 00:19:15,610
+السيطرة على إنتاج ال alpha amylase هذا سيء للطاقة
+
+247
+00:19:15,610 --> 00:19:20,830
+بالنسبة لها لكن في الآخرit is easy to make
+
+248
+00:19:20,830 --> 00:19:25,050
+mutations in bacteria لكن عكس الإنسان يقول إنه ..
+
+249
+00:19:25,050 --> 00:19:27,310
+إنه .. لإنه الـ diploid بيه عنده زوج من
+
+250
+00:19:27,310 --> 00:19:39,970
+البروموزومات وبالتالي بيكون الوضع أسلوب طيب
+
+251
+00:19:39,970 --> 00:19:43,230
+أنتوا بتعرفوا أنه الممالك تبع المخلوقات في التصنيف
+
+252
+00:19:43,230 --> 00:19:47,090
+احنا بحب التصنيف البشري و أعتقد أنه ت .. تروح
+
+253
+00:19:47,090 --> 00:19:50,950
+المكتب بعض الأحيان .. بعض الأحيانبنروح المكتبات،
+
+254
+00:19:50,950 --> 00:19:55,230
+بنلاقيش كوم كتب في المكتبات، بنلاقي أرفف وطوابق،
+
+255
+00:19:55,230 --> 00:19:57,990
+هذا طابق للمراجع باللغة الإنجليزية، للعلمية،
+
+256
+00:19:57,990 --> 00:20:01,910
+للطبية، للكيميا، للأحياء، مظلومة لتصنيفات تخيل لو
+
+257
+00:20:01,910 --> 00:20:07,070
+أنا بدي كتاب في الكيميا، و بروح لاجي كل كتب المكتب
+
+258
+00:20:07,070 --> 00:20:10,710
+كومه معاهم، يمكن ما حد ماميديا يطلع الكتاب، ويمكن
+
+259
+00:20:10,710 --> 00:20:15,110
+يطلع أخر كتاب، صح ولا لأ؟ فنحن نميل إلى التصنيف
+
+260
+00:20:15,110 --> 00:20:20,450
+والترتيب بغلط سهولة استرجاع المعلومةاسمها
+
+261
+00:20:20,450 --> 00:20:25,250
+retrieval system استرجاع المعلومات التصديق مر في
+
+262
+00:20:25,250 --> 00:20:30,590
+مراحل كتيرة على المخلوقات وفيها لحد الآن عليها
+
+263
+00:20:30,590 --> 00:20:36,110
+خلافات و الخلافات هادي هادي عن تشابك المعلومات و
+
+264
+00:20:36,110 --> 00:20:41,410
+التغيير بتم لأن معلوماتنا بتزيد يعني بعض الأحيان
+
+265
+00:20:41,410 --> 00:20:45,710
+مابنكتشفش مايكروفات جديدة ولا مخلوقات جديدة لكن
+
+266
+00:20:45,710 --> 00:20:48,720
+معلوماتنا بتزيد ليش معلوماتنا بتزيد؟لأنه في
+
+267
+00:20:48,720 --> 00:20:52,940
+research في أبحاث و لأنه في تقنيات كنا زمان ب
+
+268
+00:20:52,940 --> 00:20:55,460
+microscope عادي بعدين صرنا ب electron microscope
+
+269
+00:20:55,460 --> 00:20:59,940
+بعدين صرنا ب confocal microscopy صرنا بتقنيات
+
+270
+00:20:59,940 --> 00:21:05,520
+متطورة جدا وبتالي زادت معلومات فالتصنيف ليس يعني
+
+271
+00:21:05,520 --> 00:21:09,040
+شيء fixed ممكن يتغير و هنشوف هذا الكلام في
+
+272
+00:21:09,040 --> 00:21:12,200
+البكتيريا بعض الأحيان لكن الأنيمال أمامنا ماتغيروا
+
+273
+00:21:12,200 --> 00:21:14,340
+دلوقتي متانية أمامنا ماتغيروا animal and plant
+
+274
+00:21:14,340 --> 00:21:19,180
+ميزوا بين الحيوانات و بينالنباتات منذ زمن بعيد
+
+275
+00:21:19,180 --> 00:21:23,860
+البروتست إغلاق تشمل لبروتزوفة ومن الأشياء القريبة
+
+276
+00:21:23,860 --> 00:21:28,180
+الفنجاي اللي هي الفطريات مملكة لحالها البروكاريوت
+
+277
+00:21:28,180 --> 00:21:31,560
+عملوها مملكة وعملوا فيروسات مملكة كم مملكة صاروا
+
+278
+00:21:31,560 --> 00:21:38,320
+هذولا؟ ستة واحد اتنين دلتر اربع خمسة واحد ستة لكن
+
+279
+00:21:38,320 --> 00:21:42,940
+في شيء أعلى من المملكة أعلى من المملكة تسموه
+
+280
+00:21:42,940 --> 00:21:45,990
+domainمافي عندي domain eukaryote و eukaryote يعني
+
+281
+00:21:45,990 --> 00:21:47,970
+الـ eukaryotes لحظوا مين تحت الـ eukaryotes
+
+282
+00:21:47,970 --> 00:21:54,930
+animal, plant, protist و fungi تحت ال .. البكتيريا
+
+283
+00:21:54,930 --> 00:22:01,590
+في دمية البكتيريا أو الاركيا الوكاريوتس فقط مملكة
+
+284
+00:22:01,590 --> 00:22:07,530
+واحدة و ال viruses ليست .. ليست ضمن تصنيف ال
+
+285
+00:22:07,530 --> 00:22:11,510
+domains مملكة مالهاش domain، مملكة مالهاش domain
+
+286
+00:22:12,670 --> 00:22:17,070
+لكن اللي بتصله ان كل هذه المخلوقات اللى كوكب الأرض
+
+287
+00:22:17,070 --> 00:22:20,410
+تصنف علينها Eukaryotes وده ال mainly فقط ال
+
+288
+00:22:20,410 --> 00:22:23,130
+bacteria و ال archaea أول مرة يمول معنا مصطلح
+
+289
+00:22:23,130 --> 00:22:29,870
+archaea بنحاول نوضر مين بيعرف إيش معنى عالم آثار؟
+
+290
+00:22:29,870 --> 00:22:37,730
+لغة لإنجليزية عالم آثار archaeologist archaeology
+
+291
+00:22:37,730 --> 00:22:42,770
+يعني علم الآثار و الآثار غالبا بتكون مستقبليةقديمة
+
+292
+00:22:42,770 --> 00:22:47,830
+.. قديمة فسمى للبكتيريا Archaia لأن العلماء بتوع
+
+293
+00:22:47,830 --> 00:22:53,030
+التصنيف و بتوع التطور تمام بيقولوا انها هي الأقدم
+
+294
+00:22:53,030 --> 00:22:56,230
+في السلسلة احنا طبعا كمسلمين و انتوا عارفين ان ..
+
+295
+00:22:56,230 --> 00:23:00,670
+ان .. ان .. معتقد ان الله سبحانه وتعالى هو خلق كل
+
+296
+00:23:00,670 --> 00:23:06,210
+هذه المخلوقات مش خلق بالتطور من الى .. من الى ال
+
+297
+00:23:06,210 --> 00:23:11,090
+Archaia قلنا انه في التصنيف بيسموها البكتيريا
+
+298
+00:23:11,090 --> 00:23:16,220
+القديمةلكن بتعطيكوا بعض الأمثلة على الاركيا وعلى
+
+299
+00:23:16,220 --> 00:23:21,080
+روعتها في هذا العالم بتعرفوا البحر الميت؟ لسه مو
+
+300
+00:23:21,080 --> 00:23:27,880
+بحر ميت مافيش فيه حياة صح؟ اليوم بتقولوا أن البحر
+
+301
+00:23:27,880 --> 00:23:32,680
+الميت ظلم وسمي بحر ميت على .. على .. لدفس السيطرة
+
+302
+00:23:32,680 --> 00:23:36,660
+هو ده لسه ميت لأنه بتحيا فيه بكتيريا من الاركيا
+
+303
+00:23:36,660 --> 00:23:43,140
+اسمها halobacterium halopium وكلمة halo ملحهاله
+
+304
+00:23:43,140 --> 00:23:47,720
+معناها ملح فهي محبة للملح وبتعيش في البحر المقلور
+
+305
+00:23:47,720 --> 00:23:52,040
+بناها عندنا وحاطناها في البحر تبعنا بتموت بدها ملح
+
+306
+00:23:52,040 --> 00:23:58,820
+تمام؟ بدها كمية كبيرة من الملح لو تعرفوا في يانبيه
+
+307
+00:23:58,820 --> 00:24:02,660
+مياه سخنة ساخنة قد تصل درجة الحرارة فيها إلى تسعين
+
+308
+00:24:02,660 --> 00:24:07,000
+إلى خمس و تسعين درجة مقوية هناك تعيش بكتيريا من
+
+309
+00:24:07,000 --> 00:24:15,360
+نوع اركيا هناك كهوف كهوف في جبالبيرشح منها acid
+
+310
+00:24:15,360 --> 00:24:21,080
+مادة حمضية sulfuric acid H2SO4 لو صابت جلدنا
+
+311
+00:24:21,080 --> 00:24:27,320
+هتحرقه هنا تعيش بكتيريا تنتج هذا الحمض من نوع مين؟
+
+312
+00:24:27,320 --> 00:24:32,140
+من نوع Archa لذلك بعض العلماء بيطلقوا عليها
+
+313
+00:24:32,140 --> 00:24:39,080
+extremophileيعني البكتيريا المحبة للظروف المتطرفة،
+
+314
+00:24:39,080 --> 00:24:42,040
+البحر الميت بلوحة عالية، الحرارة العالية جدا اللي
+
+315
+00:24:42,040 --> 00:24:45,520
+بيقولون عنها في ال springs، الحمودة المرتفعة اللي
+
+316
+00:24:45,520 --> 00:24:49,360
+اتكلمنا عنها، إذا ال .. ال .. ال .. ال Archa هي
+
+317
+00:24:49,360 --> 00:24:53,560
+نوع من البكتيريا لكن غالبا بيعيش هنا في ظروف ..
+
+318
+00:24:53,560 --> 00:24:57,900
+فبتالي مش .. مش هلاجي Archa ضمن اللي هو ال medical
+
+319
+00:24:57,900 --> 00:25:00,920
+microbiome، مش هلاجي Archa ضمن ال medical
+
+320
+00:25:00,920 --> 00:25:03,760
+microbiome، هلاجي مين؟ فقط بكتيريا
+
+321
+00:25:07,860 --> 00:25:13,540
+أين نتوقع أن نجد micro organism؟ أكيد لكم أحد
+
+322
+00:25:13,540 --> 00:25:17,560
+موبايلز أه؟ فصل الماضي عملنا دراسة على ..
+
+323
+00:25:17,560 --> 00:25:20,920
+زميلاتكوا في القسم عملوا دراسة على ال mobile phone
+
+324
+00:25:20,920 --> 00:25:26,360
+و أظهروا نتائج يعني سيئة للغاية طبعا الإينات كانوا
+
+325
+00:25:26,360 --> 00:25:31,640
+أفضل من الذكور في المحافظة على نظافة ال mobile لكن
+
+326
+00:25:31,640 --> 00:25:35,270
+بشكل عام لجينا نسبةتتجاوز ال 50% من موبايلات
+
+327
+00:25:35,270 --> 00:25:41,330
+الطلبات تحتوي على potential pathogens يعني ممرضات
+
+328
+00:25:41,330 --> 00:25:48,930
+محتملة طيب بلاش نسأل السؤال بطريقة تانية عطوني
+
+329
+00:25:48,930 --> 00:25:56,410
+مكان بتعرفوا عنه أنتوا محقق الإجابة
+
+330
+00:25:56,410 --> 00:25:56,870
+بعيدة
+
+331
+00:26:00,400 --> 00:26:06,000
+أبطل عمليات هل تعقيم طبيعي و لا تعقيم صناعي تعقيم
+
+332
+00:26:06,000 --> 00:26:09,640
+الصناعي فإن حالة تعقيم طبيعي حالة معقمة يعني مثلا
+
+333
+00:26:09,640 --> 00:26:18,120
+فاكهة أكلتوها فكهة معقمة ال exterior تبع الأشياء
+
+334
+00:26:18,120 --> 00:26:21,560
+لازم يكون عليها micro wave يعني لو أخدنا منكم
+
+335
+00:26:21,560 --> 00:26:25,160
+مسحات من إيدكم و أنتم مغسلين و جايين لسه علاجة
+
+336
+00:26:25,160 --> 00:26:30,300
+micro wave تفاحة من برةمش فيها microorganism؟ فيها
+
+337
+00:26:30,300 --> 00:26:34,180
+microorganism أفضل من جوا مافيها اشي احنا أجسامنا
+
+338
+00:26:34,180 --> 00:26:39,620
+الجلد تبعنا الأنف الفم مليء بال microorganism ال
+
+339
+00:26:39,620 --> 00:26:44,400
+intestinal tract مليئة بال microorganism لكن الدم
+
+340
+00:26:44,400 --> 00:26:51,520
+تبعنا ال CSF يبقى في جسمنا احنا بنقدر نجسمه لجسمين
+
+341
+00:26:51,520 --> 00:26:56,960
+وهذا على فكرة أسهل تقسيمة في كل العلوم اللي
+
+342
+00:26:56,960 --> 00:27:00,140
+بتدرسوها في حياتكوافي الإنسان بنقسمولا جسمين
+
+343
+00:27:00,140 --> 00:27:04,940
+sterile و non-sterile parts أعضاء أو أجزاء معقمة
+
+344
+00:27:04,940 --> 00:27:07,420
+طبعا هذا تقسيم مهم لدني ليش لأن لو أنا صار عن
+
+345
+00:27:07,420 --> 00:27:11,160
+التهاب في الأنف و بدي أخد مسحة من الأنف عشان أشوف
+
+346
+00:27:11,160 --> 00:27:15,500
+المسبب للاتهاب الميكروب اللي طلع معايا الآن هل هو
+
+347
+00:27:15,500 --> 00:27:20,700
+من هو المسبب للاتهاب ولا من الموجودات الطبيعية
+
+348
+00:27:20,700 --> 00:27:23,600
+اللي بنسميها normal flora أو normal microbiota مش
+
+349
+00:27:23,600 --> 00:27:28,530
+عارفين مابنعرفش صح؟ هذه صعوبةفبنلاقي طرق للتفريق
+
+350
+00:27:28,530 --> 00:27:33,870
+بين الاتنين أمامنا ال microbes are everywhere و
+
+351
+00:27:33,870 --> 00:27:38,670
+اللي جالت الأخت اللي جالت أنه غرفة العمليات بنصرف
+
+352
+00:27:38,670 --> 00:27:42,490
+مسار كتير و فلوس كتير عشان نوفر ظروف معقمة في غرفة
+
+353
+00:27:42,490 --> 00:27:48,170
+العمليات يعني يكلفنا الأمر فلوس حتى نتخلص من ال
+
+354
+00:27:48,170 --> 00:27:53,130
+microorganism فالميكروبات دائما موجودةوما انها
+
+355
+00:27:53,130 --> 00:27:56,550
+موجودة في كل مكان ال air و ال soil و ال water و ال
+
+356
+00:27:56,550 --> 00:28:00,430
+animals و ال human body بنلاقي و احنا بنشتغل
+
+357
+00:28:00,430 --> 00:28:05,450
+microbiology فى المعامل بنلاقي طريقة مانلوثش
+
+358
+00:28:05,450 --> 00:28:12,430
+العينات مننا احنا مانتكلمش اذا انا اكون مضاف
+
+359
+00:28:12,430 --> 00:28:17,110
+مطهرين تمام نشتغل جنب اللهب مانتخلش الهوى اللى
+
+360
+00:28:17,110 --> 00:28:24,180
+موجود ينزل مانتكلمش مانتنفسشفي الطبخ تبعنا تبعا كل
+
+361
+00:28:24,180 --> 00:28:28,320
+هذه أشياء ممارسات يجب أن نتعلمها في المعمل حتى
+
+362
+00:28:28,320 --> 00:28:34,620
+نتجنب ان نطلع نتائج خاطئة في ال microbiology طبعا
+
+363
+00:28:34,620 --> 00:28:38,080
+انا بقول دايما ان ال soil هي المصدر الأساسي للتربة
+
+364
+00:28:38,080 --> 00:28:43,180
+لكن من ال soil تعرف ان الهواء بيلامس التربة بياخد
+
+365
+00:28:43,180 --> 00:28:46,880
+معاها غبار و غبار في أين بيسمع ان؟ الهواء الهواء
+
+366
+00:28:46,880 --> 00:28:53,910
+لا يوفر بيئةده يعني رطوبة مناسبة أو تغذية مناسبة
+
+367
+00:28:53,910 --> 00:28:57,690
+تالي هو مجرد carrier للميكروبات حامل للميكروبات
+
+368
+00:28:57,690 --> 00:29:02,210
+ينقلها من مكان إلى مكان بس التربة فيها مادة عضوية
+
+369
+00:29:02,210 --> 00:29:06,090
+وفيها رطوبة قد توفر بيه ألمين لنمو المايكرو
+
+370
+00:29:06,090 --> 00:29:12,270
+أورجانز ال water مش تشتت دنيا تاخد معاها تربة و
+
+371
+00:29:12,270 --> 00:29:16,070
+تروح عليها بشكل streams و تروح علي نهار أو علي
+
+372
+00:29:16,070 --> 00:29:19,130
+البحارال animals و ال human bodies احنا فى ال
+
+373
+00:29:19,130 --> 00:29:24,650
+intestinal tract بتاعنا one gram one gram of stool
+
+374
+00:29:24,650 --> 00:29:30,250
+contains ten to the power of nine او twelve يعني
+
+375
+00:29:30,250 --> 00:29:34,010
+عشرة اقص تسعة لعشرة اقص اتناش micro organism ده كل
+
+376
+00:29:34,010 --> 00:29:38,810
+gram ايش رأيك فى الرقم؟ رقم مهول ال saliva اللى
+
+377
+00:29:38,810 --> 00:29:43,970
+وعاب تبع الإنسان الواحد ملي منه بيحتوي على عشرة
+
+378
+00:29:43,970 --> 00:29:48,190
+اقص تسعة micro organism بتاع التقولي فرشية أسناني
+
+379
+00:29:48,190 --> 00:29:52,310
+مفرشية أسناني أه؟ أصلا الناس المهووسين بأنهم
+
+380
+00:29:52,310 --> 00:29:55,250
+يفرشوا أسنانهم باستمرار بيصيروا عندهم أمراض يعني
+
+381
+00:29:55,250 --> 00:29:58,950
+الفم يجب أن يحتوي على micro organism يجب أن يحتوي
+
+382
+00:29:58,950 --> 00:30:03,430
+على .. مش معناه أضحك أنه نسيبه عفن لأ المقصود فيه
+
+383
+00:30:03,430 --> 00:30:08,370
+أنه يعني لا مبالغة ولا تتفرض في ال .. فال human
+
+384
+00:30:08,370 --> 00:30:11,810
+body و ال animal body بتحتوي على micro organism
+
+385
+00:30:11,810 --> 00:30:17,790
+بعداد ضخمة جداهذا مثال على garden soil بيقولنا
+
+386
+00:30:17,790 --> 00:30:21,230
+محتوى على تسعة أو سبعة من عشرة ضرب عشرة او اذا كده
+
+387
+00:30:21,230 --> 00:30:26,410
+شهرين ممكن ستة او سبعة micro organism من التربة
+
+388
+00:30:26,410 --> 00:30:31,790
+اللي تبعت المنزل طبعا ايش الميزة ما بين تربة و ما
+
+389
+00:30:31,790 --> 00:30:37,090
+بين انسان او او او انسان مصعب بداية بنفهم مصطلح
+
+390
+00:30:37,090 --> 00:30:40,950
+كتير مهم اسمه competition الان انتوا في الفصل هنا
+
+391
+00:30:40,950 --> 00:30:46,170
+لو كنتوا عشر اشخاصفرصتك في انكوا تسألوا سؤال ايه
+
+392
+00:30:46,170 --> 00:30:50,470
+ليه واحد من عشرة صح ولا لا فرصة اي واحدة منكم طب
+
+393
+00:30:50,470 --> 00:30:54,250
+لو زاد العدد ساره خمسين بصير فرصتك واحد من خمسين
+
+394
+00:30:54,250 --> 00:31:01,630
+فالمنافسة بتزيد فبالتالي انت معرفتك بتجل معرفتك
+
+395
+00:31:01,630 --> 00:31:06,510
+بتجل فوق أقصد فرصك بتجل في ال .. في البيئة
+
+396
+00:31:06,510 --> 00:31:12,130
+الحقيقية الميكروبات بتكون في حالة تنافس ميكروب
+
+397
+00:31:12,130 --> 00:31:16,190
+وميكروب وميكروب وميكروببتنفسوا مع بعض وكل مايكروب
+
+398
+00:31:16,190 --> 00:31:19,490
+فيه استراتيجياته في الدفاع و استراتيجياته في
+
+399
+00:31:19,490 --> 00:31:25,730
+الهجوم لكن لو واحد من هذه الميكروبات أخدنا لحاله
+
+400
+00:31:25,730 --> 00:31:30,390
+من المجموعة و حطناها في بيئة مافيش فيها تنافس خرصه
+
+401
+00:31:30,390 --> 00:31:34,430
+بتزيد او بتزيد اش؟ بتزيد وبالتالي احنا بنحاول برضه
+
+402
+00:31:34,430 --> 00:31:38,910
+لما بنقزل مايكروب من environment او من clinical
+
+403
+00:31:38,910 --> 00:31:44,930
+sampleإنه ينشيل عنه الأخرين عشان نوفرله فرصة عالية
+
+404
+00:31:44,930 --> 00:31:48,170
+فال competition بتكون أقل في الحالة و الفرصة بتكون
+
+405
+00:31:48,170 --> 00:31:54,290
+أفضل هذه ال .. هذه المادج على إنه أرقام البكتيريا
+
+406
+00:31:54,290 --> 00:31:58,190
+بتتراوح بحسب المادة اللي موجودة فيها مثلا أنا عندي
+
+407
+00:31:58,190 --> 00:32:04,570
+أنا cheese ال
+
+408
+00:32:04,570 --> 00:32:08,970
+milk طبعا ال milk ال raw milk اللي مش مبسطةقد يصل
+
+409
+00:32:08,970 --> 00:32:13,230
+فيها من 1¹⁸ لكل جرافة لكن بعد ما نبسطره و نبسطره
+
+410
+00:32:13,230 --> 00:32:16,350
+ليست عملية تعقيم خدوا بالك و انما عملية قتل للبعض
+
+411
+00:32:16,350 --> 00:32:19,710
+من انك micro organism لذا انا في اندي تراوح و
+
+412
+00:32:19,710 --> 00:32:24,010
+تثاؤت في ال micro organism من surface إلى surface
+
+413
+00:32:24,010 --> 00:32:29,930
+طيب قبل ما ابدأ اتكلم باللوحة الفيكوسية اللي قطعت
+
+414
+00:32:29,930 --> 00:32:33,510
+عنها و ما اتحدث عنها بدنا نحاول الان نرجع إليها
+
+415
+00:32:34,710 --> 00:32:37,770
+وقولنا ان ال micro organism are with great
+
+416
+00:32:37,770 --> 00:32:43,670
+biodiversity ايش يعني biodiversity؟ تنوع حيوي
+
+417
+00:32:43,670 --> 00:32:48,510
+مهمول، بدنا نستغل هذه المعلومة، نعرفها وشو هي تنوع
+
+418
+00:32:48,510 --> 00:32:51,450
+حيوي وإيش يعني ممكن أو كيف ممكن نستغل هذه
+
+419
+00:32:51,450 --> 00:32:57,950
+المعلومة؟ احنا على المستوى البشري طعامنا اللي
+
+420
+00:32:57,950 --> 00:33:01,230
+قدامنا اللي بنشوفه إما نباتات أو حيوانة، صح هذا
+
+421
+00:33:01,230 --> 00:33:06,900
+لأ؟وطبعا أصناف محددة منه مش كل إشي كده المايكروبات
+
+422
+00:33:06,900 --> 00:33:11,840
+لما أنا قلت إنها موجودة everywhere في البحر الميت
+
+423
+00:33:11,840 --> 00:33:16,660
+وفي الكهوف الحمضية وفي الميه أقولكوا أبتدعوا الميه
+
+424
+00:33:16,660 --> 00:33:21,140
+المادانية اللي بيشرب هذي لما فيها مايكروبات أنا مش
+
+425
+00:33:21,140 --> 00:33:24,600
+بنقولكوا إن كل .. كل العالم موجود مكتير .. قلتلكوا
+
+426
+00:33:24,600 --> 00:33:28,760
+قلت 98% من المايكروبات أصلا مش ضار يعني 2% فقط في
+
+427
+00:33:28,760 --> 00:33:33,370
+الميه 1% من المايكروبات هو اللي ضارلكن وجود
+
+428
+00:33:33,370 --> 00:33:36,730
+الميكروبات هذه في بيئات متنوعة ومختلفة، ماذا يعني
+
+429
+00:33:36,730 --> 00:33:43,270
+لكم؟ يعني مقدرة هذه الميكروبات المتنوعة على تناول
+
+430
+00:33:43,270 --> 00:33:50,190
+أغذية متنوعة طب أقولكم مثال مضطرف جدا، في بكتيريا
+
+431
+00:33:50,190 --> 00:33:54,710
+متعيش في درجة حرارة قد تصل إلى 200 درجة مأوية، وين
+
+432
+00:33:54,710 --> 00:33:57,570
+في عصرها الأرض 200 درجة مأوية؟ في الشيء، تحت قاع
+
+433
+00:33:57,570 --> 00:34:04,000
+المحيطاتبتشوفوا ان كان في ال .. في الوثائقيات اشي
+
+434
+00:34:04,000 --> 00:34:07,740
+بيطلع من تحت المحيط بيسموها thermal vents فتحات هي
+
+435
+00:34:07,740 --> 00:34:11,760
+عبارة عن فتحات بوركانية اللي .. اللي بيطلع منها
+
+436
+00:34:11,760 --> 00:34:15,620
+بيكون طبعا ساخن جدا و لإن المحيط ال .. ال column
+
+437
+00:34:15,620 --> 00:34:19,400
+تبع الماء عال جدا فبشكل ضغط فالماء قد يصل درجة حرط
+
+438
+00:34:19,400 --> 00:34:22,460
+هناك فارق المنطقة إلى 200 درجة أو .. أو حتى أعلى
+
+439
+00:34:22,460 --> 00:34:27,360
+من ذلك ولا يوجد أي نوع من الضوء حتى نقول إن ال
+
+440
+00:34:27,360 --> 00:34:30,100
+bacterium تم ال photosynthesisاللي هو تمثيل ضوء
+
+441
+00:34:30,100 --> 00:34:35,860
+وانما تقتات تتغذى على الكابريت الخارج من هذه
+
+442
+00:34:35,860 --> 00:34:42,060
+الفتحات بس على الكابريت باشي sun light باشي sun
+
+443
+00:34:42,060 --> 00:34:47,380
+light إذا تنوع التغذية في البكتيريا من نوع إلى آخر
+
+444
+00:34:47,380 --> 00:34:51,120
+تمام قد يفيدنا كيف بيفيدنا؟ في التعرف و التمييز
+
+445
+00:34:51,120 --> 00:34:55,840
+بينهمبتستخدمي واحد اتنين تلاتة يا بيستخدم واحد
+
+446
+00:34:55,840 --> 00:35:00,400
+واربعة وسبعة يا بيستخدم تلاتة واتنين وواحد يبقى
+
+447
+00:35:00,400 --> 00:35:03,500
+بقدر اميزكوا عن بعض دي الحلقة ابقولكوا كيف الفكرة
+
+448
+00:35:03,500 --> 00:35:07,440
+تبعها تحت ال microscope اسموا الأسماء اللي بتحكيها
+
+449
+00:35:07,440 --> 00:35:12,680
+مش للحفظ وانما لالمثال Salmonella type,
+
+450
+00:35:12,820 --> 00:35:16,580
+Escherichia coli, Sodomonas aeruginosa, Shigella,
+
+451
+00:35:18,080 --> 00:35:22,410
+Proteus, Morganella, Edward Sella اسمها حلوةبعد
+
+452
+00:35:22,410 --> 00:35:25,210
+فترة هتعودوا عليها و هتسموا بناتكم زيها في
+
+453
+00:35:25,210 --> 00:35:31,250
+المستقبل كل الأسماء اللي ذكرتهم و جابتهم متين اسم
+
+454
+00:35:31,250 --> 00:35:36,550
+تحت الميكروسكوب يبدو و كأنهم شحطة حمراء بقى، زي ..
+
+455
+00:35:36,550 --> 00:35:44,090
+لونها أحمر و عصايا حمراء كيف نميز إيشريشيا كولار
+
+456
+00:35:44,090 --> 00:35:46,290
+عن سلمونيلا عن شيجيلا عن الأسماء اللي احنا
+
+457
+00:35:46,290 --> 00:35:50,860
+ذكرناهم؟نستطيع تمييزهم من خلال ال biodiversity من
+
+458
+00:35:50,860 --> 00:35:54,020
+خلال تنموها هون في التغيير زى ما قلنا لكوا اشهر
+
+459
+00:35:54,020 --> 00:35:57,480
+بيخمر ال lactose اشهر بيخمرش ال lactose اشهر
+
+460
+00:35:57,480 --> 00:36:01,880
+بيستخدم ال glucose اشهر بيستخدم ال sucrose و هكذا
+
+461
+00:36:01,880 --> 00:36:06,000
+يعني بندير على الفروقات في ال metabolism وهذا
+
+462
+00:36:06,000 --> 00:36:08,940
+هيشكل جزء كبير من النعمل تبعكم السنة هذه ان شاء
+
+463
+00:36:08,940 --> 00:36:12,060
+الله هتعرفوا على كيف بنميز ال micro organism
+
+464
+00:36:12,060 --> 00:36:15,260
+بالفحوصات اللي بيروح كيميائية طبعا هسهرنا برضه ال
+
+465
+00:36:15,260 --> 00:36:18,860
+ground stateإن ان تشوفوا البكتيريا شحتة حمراء،
+
+466
+00:36:18,860 --> 00:36:21,820
+ليه؟ لكي تعرفوا أن هذه واحدة من هذه المجموعات،
+
+467
+00:36:21,820 --> 00:36:23,440
+واحدة من هذه المجموعة.
+
+468
+00:36:29,420 --> 00:36:33,700
+هذه يمكن اللوحة اللي قدامنا بتوضح لنا دور ال micro
+
+469
+00:36:33,700 --> 00:36:36,960
+organism في ال nitrogen cycle، nitrogen cycle،
+
+470
+00:36:36,960 --> 00:36:40,400
+اللي هي دائرة ال nitrogen. أنا بتقولكوا من بداية
+
+471
+00:36:40,400 --> 00:36:44,980
+المحاضرة إن تدوير العناصر، تدوير العناصر هو الدزء
+
+472
+00:36:44,980 --> 00:36:49,120
+أساسيمن مهام ال micro organism و أقولكوا جملة
+
+473
+00:36:49,120 --> 00:36:52,520
+أخبرنا بيها أحد الدكاترات اللي درسونا احنا فيبقى
+
+474
+00:36:52,520 --> 00:36:56,480
+ننقلها لكم ان شاء الله كلمة لطيفة و لذيذة بتعبر عن
+
+475
+00:36:56,480 --> 00:36:59,580
+الواقع اللي بنقدر نقصد فيه ال micro organism
+
+476
+00:36:59,580 --> 00:37:05,080
+بيقولوا لا تظلموا الميكرو بقى هذا المقدمة تبعكوا
+
+477
+00:37:05,080 --> 00:37:09,020
+لا تظلموا الميكرو بقى their role in nature is to
+
+478
+00:37:09,020 --> 00:37:13,280
+convert large molecules into small molecules دورها
+
+479
+00:37:13,280 --> 00:37:16,900
+في الحياةدورة الحياة أن تحول الجزيئات الكبيرة
+
+480
+00:37:16,900 --> 00:37:21,300
+لجزيئات صغيرة doing their thing يعني doing their
+
+481
+00:37:21,300 --> 00:37:26,780
+job يعني خلال عملها they may fall in our food and
+
+482
+00:37:26,780 --> 00:37:32,320
+spoil it قد تقع مش .. مش جاسدها هي بس إذا هي قعدت
+
+483
+00:37:32,320 --> 00:37:36,200
+وينما .. وينما نحطها ابتدوا ويتكسر انتهى الموضوع
+
+484
+00:37:36,200 --> 00:37:40,340
+هي دورة الحياة if they enter our body they might
+
+485
+00:37:40,340 --> 00:37:44,460
+cause diseasesإذا لا تظلموا الميكروفات تعمل عملها
+
+486
+00:37:44,460 --> 00:37:50,500
+و هي تحويل الجزيئات الكبيرة إلى جزيئات صغيرة فهذه
+
+487
+00:37:50,500 --> 00:37:54,220
+نقطة كتير مهمة و هذه طبعا أنا مش مش مش كتير مهتمين
+
+488
+00:37:54,220 --> 00:37:56,820
+بإنه كنا نعرف كيف ال nitrogen سجل لكن في الأخر هي
+
+489
+00:37:56,820 --> 00:38:01,660
+عملية تحل كنت عارف إن الميكروفات كمان ابتغى من
+
+490
+00:38:01,660 --> 00:38:05,800
+nitrogen fixation يعني
+
+491
+00:38:05,800 --> 00:38:10,860
+الزراعة استفادت بشكل كبير من هذه الحقيقةغير
+
+492
+00:38:10,860 --> 00:38:13,960
+البكتيريا اللى بتكون موجودة في الجذور اللى أصلا
+
+493
+00:38:13,960 --> 00:38:17,900
+دراستوها نباتات دواة الجذور العقدية اللى بيكون
+
+494
+00:38:17,900 --> 00:38:22,040
+فيها عقد وهذا العقد أبقى عنها عن بكتيريا عايشة جوا
+
+495
+00:38:22,040 --> 00:38:25,420
+هذه العقد بعلاقة بيسميها symbiosis اللى هي علاقة
+
+496
+00:38:25,420 --> 00:38:30,760
+تكافوية بين النبات وبين البكتيريا هناك free living
+
+497
+00:38:30,760 --> 00:38:34,400
+bacteria ممكن تثبت ال nitrogen لو نميناها في
+
+498
+00:38:34,400 --> 00:38:38,740
+المختبر ورشيناها على أراضي زراعية مش احنا هو كأنه
+
+499
+00:38:38,740 --> 00:38:42,750
+أرفنا سماد عضوي عليهايعني بمعنى أخر أن البكتيريا
+
+500
+00:38:42,750 --> 00:38:48,470
+لها دورة .. دورة عظيمة في هذا الموضوع لكن برضه
+
+501
+00:38:48,470 --> 00:38:52,850
+شايفين أن هذا .. هذا عبارة عن necrosis قدم للمريض
+
+502
+00:38:52,850 --> 00:38:59,390
+وفي أن صار فيها اللي هو تحلل أو تاكل أو موضوع إيه
+
+503
+00:38:59,390 --> 00:39:03,430
+الخلايا فهذا الهرمش بيقولنا not all bacteria cause
+
+504
+00:39:03,430 --> 00:39:06,550
+disease الوزر أعظم اللي هو قاعة الهرم كلها non
+
+505
+00:39:06,550 --> 00:39:10,570
+pathogenic غير مفيدةالبعض منها potentially زي ما
+
+506
+00:39:10,570 --> 00:39:15,570
+قلتلكوا ممكن يمرض ممكن مامرضش والبعض الأخر موصوم
+
+507
+00:39:15,570 --> 00:39:23,730
+عليه علامة أن هذا دائما ممرض نعرف
+
+508
+00:39:23,730 --> 00:39:26,250
+كلمة مرة لأ بكافة طب اللي خليني أتكلم عن التعريف
+
+509
+00:39:26,250 --> 00:39:33,450
+يعني بشكل تفصيل شوية كلمة evolution أحنا قلتلكوا
+
+510
+00:39:33,450 --> 00:39:38,760
+مش متفقين فيها أن هو موضوع تطورclassification لأ
+
+511
+00:39:38,760 --> 00:39:41,040
+متفقين في ال classification التصنيف كتير مهم من
+
+512
+00:39:41,040 --> 00:39:44,800
+التصنيف ليش تصنيف مهم التصنيف مهم قولنالكوا اللي
+
+513
+00:39:44,800 --> 00:39:51,580
+غرض استرجاع المعلومة طالع لي هنا عندي شخص دخل
+
+514
+00:39:51,580 --> 00:39:54,820
+الباب و جال دكتور فيه طالب من طالباتك أخدت معاه
+
+515
+00:39:54,820 --> 00:39:58,560
+مني ورقة و أنا مش هعرفها مش هعرف أي سنة طب
+
+516
+00:39:58,560 --> 00:40:03,900
+وصفينيها جاتيها طالبة بطلعتكوا بيقولكوا ليه طالبات
+
+517
+00:40:03,900 --> 00:40:06,060
+فبالتالي
+
+518
+00:40:07,100 --> 00:40:10,920
+أعطتني معلومة أكدتلي انه انا بدوّف المكان الصحيح
+
+519
+00:40:10,920 --> 00:40:16,840
+رقم اتنين ايش كمان ماجات لابسها تلبس نضارة الان
+
+520
+00:40:16,840 --> 00:40:22,140
+انا بدوّف عاملها على لابسين النضارة وبستخني الجميع
+
+521
+00:40:22,140 --> 00:40:27,600
+لآخر اكمله حضر واحد اتنين تلاتة تلاتة استثنين
+
+522
+00:40:27,600 --> 00:40:33,320
+الاربع استثنين الباقي طب كمان معلومة بتبقى تعطيني
+
+523
+00:40:33,320 --> 00:40:38,260
+معلومات لما أصل للشخص الصحيحالان انا شايف good DNA
+
+524
+00:40:38,260 --> 00:40:43,280
+صح بس ال bacteria بشوفهاش good DNA بشوفها بذاكره
+
+525
+00:40:43,280 --> 00:40:50,060
+بمعنى بتجمعلها معلومات معلومات نظرية بصفاتها يعني
+
+526
+00:40:50,060 --> 00:40:51,960
+من اخر الفصل اللي جاي في ال diagnostic
+
+527
+00:40:51,960 --> 00:40:55,960
+microbiology هتحفظوا إيه شكولايك كرام positive ولا
+
+528
+00:40:55,960 --> 00:40:59,980
+كرام negative بس اللي ولا كوكسايد إجليكوز negative
+
+529
+00:40:59,980 --> 00:41:03,040
+ولا إجليكوز positive لا الأكتوز positive ولا
+
+530
+00:41:03,040 --> 00:41:08,670
+الأكتوز negativeإن دول ميتيل ريدف و بروسكاوه أسماء
+
+531
+00:41:08,670 --> 00:41:13,950
+عديدة صفات عديدة لميكروب واحد بنخزينهم في وين في
+
+532
+00:41:13,950 --> 00:41:18,330
+الباكره و بنصير لما نشوفهم بنسترجع و نقارن إذا
+
+533
+00:41:18,330 --> 00:41:22,010
+أقول إن أنا مش قادر أسترهيب بنستبدلها بإيش ببرامج
+
+534
+00:41:22,010 --> 00:41:25,750
+حسوب برامج حسوب بندخله البياتة بدء من الحفاظ
+
+535
+00:41:25,750 --> 00:41:29,190
+اللحظة بندخلها بالحسوب و لما بيجينا ميكروب يقولي
+
+536
+00:41:29,190 --> 00:41:33,170
+بندخله البياتة صفاته بقول your microbe is كده و
+
+537
+00:41:33,170 --> 00:41:37,380
+كده وصل الصورةفعملية التعرف للميكروهيزم بتتم
+
+538
+00:41:37,380 --> 00:41:41,740
+بالاستثناء لكن بالأساس معظم العالمين على ال
+
+539
+00:41:41,740 --> 00:41:45,260
+classification كلمة morphology اللي هو ده اعتماد
+
+540
+00:41:45,260 --> 00:41:47,980
+الشكل هو احنا بقولك البكتيريا very simple ياما
+
+541
+00:41:47,980 --> 00:41:52,480
+عصوية ياما كراوية ياما الهي اسباية physiology
+
+542
+00:41:52,480 --> 00:41:56,320
+التغذية تبعتها أنا بترجع بشوية كيف تتغذى و كيف
+
+543
+00:41:56,320 --> 00:42:00,960
+تنمو التغذية تبعتها هل تستخدم ال substrate X ولا
+
+544
+00:42:00,960 --> 00:42:06,140
+ال substrate Y الاخرىالـ Genetics تبعتها بعض
+
+545
+00:42:06,140 --> 00:42:12,700
+الأحيان .. طبعا هنا قلنا قبل شوية أنه هناك تغيير
+
+546
+00:42:12,700 --> 00:42:16,360
+مستمر في التصنيف تبع الميكروبات مستثمر في درجة أن
+
+547
+00:42:16,360 --> 00:42:21,360
+معروفاتنا بتزيد في السنوات الأخيرة علم الوراطة
+
+548
+00:42:21,360 --> 00:42:26,080
+الجزائية قفز قفزات نوعية وبالتالي بالفعل ساعدنا في
+
+549
+00:42:26,080 --> 00:42:29,060
+عملية اللي هو التصنيف مرة أخرى ال ecology البيئة
+
+550
+00:42:29,060 --> 00:42:32,480
+تبعة الميكروبات under certain definite conditions
+
+551
+00:42:32,600 --> 00:42:38,080
+تحت ظروف اللي هو معين تاني كمان هنا شغل تانية the
+
+552
+00:42:38,080 --> 00:42:43,580
+law of their life activities ما النظام اللي يسيطر
+
+553
+00:42:43,580 --> 00:42:47,940
+أو يحكم تصرف اللي هو ال micro organism and their
+
+554
+00:42:47,940 --> 00:42:51,520
+interaction with the human being كيف نتفاعل مع
+
+555
+00:42:51,520 --> 00:42:58,610
+البشرسلبا، إيجابا، فائدة، mutualism يعني اتفاد
+
+556
+00:42:58,610 --> 00:43:03,210
+منفعة و لا harmful relation هذا كلها ضمن ال micro
+
+557
+00:43:03,210 --> 00:43:07,030
+environment طبعا interaction with human beings
+
+558
+00:43:07,030 --> 00:43:10,270
+animates our plan as well as natural environment
+
+559
+00:43:10,270 --> 00:43:18,890
+انتوا بتعرفوا انه كتير من البيئات فسدت بسبب طمع
+
+560
+00:43:18,890 --> 00:43:24,520
+البشر خدوها قاعدة أينما وجد الماءعلى .. تاريخيا
+
+561
+00:43:24,520 --> 00:43:28,560
+استوطن الناس .. استوطن الناس و بتعرف كل الحضارات
+
+562
+00:43:28,560 --> 00:43:34,100
+نجحت على الأوضيه و الأنهار و أينما حل الإنسان و
+
+563
+00:43:34,100 --> 00:43:38,880
+استوطن حل الفساد الشيء كان إيه؟ لإنه بيطلع البحر
+
+564
+00:43:38,880 --> 00:43:41,520
+.. قطع الميه .. بيجيه الميه جارية .. بيصيب
+
+565
+00:43:41,520 --> 00:43:45,080
+النفايات تبعته يحطها .. ماشي النفايات الميه
+
+566
+00:43:45,080 --> 00:43:48,220
+الجارية هيخدها للتطبيق المعين .. لكن لإن حجم
+
+567
+00:43:48,220 --> 00:43:54,160
+النفايات بيزيدبنسميها زاد أو فاق عن قدرة الـ self
+
+568
+00:43:54,160 --> 00:43:57,080
+-purification ليها التنقية الذاتية، ليها ده بيسب
+
+569
+00:43:57,080 --> 00:44:01,200
+من الماء وبالتالي بيفسد، وبالتالي بيفسد فال
+
+570
+00:44:01,200 --> 00:44:06,440
+interaction ما بين الإنسان و ال environment قد
+
+571
+00:44:06,440 --> 00:44:09,460
+يكون واصل بينهم ال micro organism يعني كتير من
+
+572
+00:44:09,460 --> 00:44:14,340
+البيئة استرجعناها بعد ما فسدت باستخدام micro
+
+573
+00:44:14,340 --> 00:44:19,060
+organism بنحط فيها micro organism خلطات معينةكي
+
+574
+00:44:19,060 --> 00:44:23,440
+بتسترجعنا البيئة، بتكسر المواد الضارة، بتشيل بعض
+
+575
+00:44:23,440 --> 00:44:29,700
+السموم من البيئة، وبالتالي بنرجع البيئة بشكل جيد
+
+576
+00:44:29,700 --> 00:44:35,420
+أنا قلت انه كتير ميكروباليجي مهمة في حياتنا، مهمة
+
+577
+00:44:35,420 --> 00:44:38,160
+في ال environment، مهمة في الطب، غير انها بتسببنا
+
+578
+00:44:38,160 --> 00:44:43,560
+امراض، علاجات، بنفلق منها، في ال food الإنسان عرف
+
+579
+00:44:43,560 --> 00:44:48,280
+الميكروبات، خدوا بالكوا، عرف الميكروبات قبل العلم
+
+580
+00:44:48,280 --> 00:44:52,840
+الحديدالمصريين القدامى لا عندهم microscope ولا
+
+581
+00:44:52,840 --> 00:44:56,800
+عندهم أي أجزة من الأجزة اللى .. اللى موجودة و بعد
+
+582
+00:44:56,800 --> 00:45:00,440
+ذلك صنعوا الخبز و الخبز معروف أنه بتدخل بصناعته
+
+583
+00:45:00,440 --> 00:45:04,840
+مين الخميرة و الخميرة micro organism و لحد وقت
+
+584
+00:45:04,840 --> 00:45:10,120
+قريب كانوا يعني مش .. مش أجدادكوا يعني أباهم ..
+
+585
+00:45:10,120 --> 00:45:14,580
+أبا أجدادكوا كانوا العجين اللى بيعجنوه في البيت مش
+
+586
+00:45:14,580 --> 00:45:20,180
+بيشتروه خميرة بيخلوا جزء من عجين مباركتمام؟ و
+
+587
+00:45:20,180 --> 00:45:25,960
+بخلطوه مع عجين بكرة يعني بياخدوا الخميرة لمن؟ بنمو
+
+588
+00:45:25,960 --> 00:45:29,780
+الخميرة من عليه نفسه هذا بيصير مصدر الخميرة بيحطوه
+
+589
+00:45:29,780 --> 00:45:32,840
+ل .. ل .. ل .. فهذا الكلام قبل ما يعرفوا حاجة من
+
+590
+00:45:32,840 --> 00:45:36,980
+ال micro بيقولوا ان المصريين القدماء استخدموا
+
+591
+00:45:36,980 --> 00:45:42,500
+الخبز لمعفن تمام؟ لمدوّة الجروح العفن هذا هو
+
+592
+00:45:42,500 --> 00:45:48,500
+penicillin يبقى الإنسان شعر بي ..قيمة ال micro
+
+593
+00:45:48,500 --> 00:45:52,020
+organism قبل حتى اللي هو الاتشار في العلم الحديث
+
+594
+00:45:52,020 --> 00:45:55,380
+عن ال micro bio في الفوق ال industry ال bio
+
+595
+00:45:55,380 --> 00:45:58,840
+technology و ال research كلمة bio technology تسمع
+
+596
+00:45:58,840 --> 00:46:04,900
+ان فينا جسم هنا في ال GMO اسمه bio technology عشان
+
+597
+00:46:04,900 --> 00:46:09,160
+انا اقولكم جداش مهم ال micro vat في ال bio
+
+598
+00:46:09,160 --> 00:46:11,220
+technology فيه فرق في ال bio technology اسمه
+
+599
+00:46:11,220 --> 00:46:15,140
+industrial microbiome industrial microbiome جزء من
+
+600
+00:46:15,140 --> 00:46:20,130
+مين؟ من ال bio technologyيقدر ال industrial
+
+601
+00:46:20,130 --> 00:46:23,010
+microbiology investment اللي هو الاستثمار اللي
+
+602
+00:46:23,010 --> 00:46:27,330
+قاعد فيه في العالم ببلايين الدولارات سنوية فقطعهم
+
+603
+00:46:27,330 --> 00:46:31,450
+هفر ده طبعا ال research دايما بيقولوا في الطب the
+
+604
+00:46:31,450 --> 00:46:34,990
+research of today is the medicine of tomorrow
+
+605
+00:46:34,990 --> 00:46:38,570
+يعنيش معناها معناه يقولوا بيه البحث اليوم بيصير
+
+606
+00:46:38,570 --> 00:46:43,170
+الطب .. بيصير الطب لل .. لل .. ده نموذج على تصنيع
+
+607
+00:46:43,170 --> 00:46:47,850
+نستكلم عنهأنا قلت ممكن يوجهوا شوية لقصة طرفنا اللي
+
+608
+00:46:47,850 --> 00:46:52,170
+له حق بالطريق إن الإنسان شعر بي ال .. ال ..
+
+609
+00:46:52,170 --> 00:46:57,430
+الميكروبات و استخدمها قبل ما يعرفها و بعدين بدأ ي
+
+610
+00:46:57,430 --> 00:47:01,490
+.. يتعرف عليها و الآن في أنها طور بالفعل modern
+
+611
+00:47:01,490 --> 00:47:05,570
+جدا و متعامل مع .. إن احنا متعامل مع الميكروبات و
+
+612
+00:47:05,570 --> 00:47:12,550
+كأنها دواجن بننميها أو كأنها أبقار بنربيهابنتعامل
+
+613
+00:47:12,550 --> 00:47:17,430
+معها بطرق قياسية سهلة جدا و رخيصة جدا و بنستغرب
+
+614
+00:47:17,430 --> 00:47:21,910
+منها بشكل كام من
+
+615
+00:47:21,910 --> 00:47:26,650
+الناس اللي أثروا علم ال microbiology Anthony van
+
+616
+00:47:26,650 --> 00:47:32,030
+Leeuwenhoek اللي اخترع اللي هو ال microscope و
+
+617
+00:47:32,030 --> 00:47:37,770
+Louise Pastier اللي كان البسطر على اسمه تمام و
+
+618
+00:47:37,770 --> 00:47:43,770
+Robert Koch اللي عمل فرضيةكوخ سموها كوخ postulate
+
+619
+00:47:43,770 --> 00:47:47,410
+هتكلم عنها انشاء الله لاحقا بليستر اللي هو اتكلم
+
+620
+00:47:47,410 --> 00:47:50,950
+على ال sterilization of this infection اللي هو
+
+621
+00:47:50,950 --> 00:47:56,310
+عامل تعقيمه في التطهير هذه طبعا بعض الشخصيات اللي
+
+622
+00:47:56,310 --> 00:47:58,870
+انا ذكرتها هذا Anthony van Leeuwenhoek وهي ال
+
+623
+00:47:58,870 --> 00:48:06,650
+microscope تبعه اللي هو اللي عمله هذا
+
+624
+00:48:06,650 --> 00:48:11,820
+مين؟ هذا Edward JennerEdward Jenner هذا اللي كان
+
+625
+00:48:11,820 --> 00:48:15,620
+مسئول عن تطوير اللقاحات و ال vaccines اللي احنا
+
+626
+00:48:15,620 --> 00:48:19,020
+بنعرفها او التطييمات اللي بنعرفها في العصر الحديث
+
+627
+00:48:19,020 --> 00:48:23,220
+طبعا التطييمات كانت موقفة سابقا الصينيين عرفوها و
+
+628
+00:48:23,220 --> 00:48:28,540
+العرب عرفوها لكن ماعرفوهاش في طور المدن ولا في ال
+
+629
+00:48:28,540 --> 00:48:31,600
+experimental عرفوها في ال pre اللي هي المرحلة
+
+630
+00:48:31,600 --> 00:48:33,860
+الأولى اللي قلنالكوا عملنا خبز و احنا مش عارفين
+
+631
+00:48:33,860 --> 00:48:39,780
+ليش الخبز صار هيك نفس ال case كان فيهحاجة لطيفة في
+
+632
+00:48:39,780 --> 00:48:44,140
+السعودية و العراق في مرة اسمه Baghdad Sour قرحة
+
+633
+00:48:44,140 --> 00:48:47,760
+بغداد .. قرحة بغداد هنا فيها ريحة مولودة في في
+
+634
+00:48:47,760 --> 00:48:52,640
+فلسطين عبارة عن ذبابة بتعض ال .. ال .. ال ..
+
+635
+00:48:52,640 --> 00:48:56,180
+السابق تنقله برازايد اسمه Leishmania هان ال
+
+636
+00:48:56,180 --> 00:49:00,480
+Leishmania مشهورة في اليمن و السعودية و العراق لما
+
+637
+00:49:00,480 --> 00:49:06,020
+تصيب الفتاة في الوجه مثلا تعملها بجعة دائرية كويس
+
+638
+00:49:06,020 --> 00:49:11,450
+trademark علامةخلاص هذه بتشوه اللي هو الشكل فكانت
+
+639
+00:49:11,450 --> 00:49:16,390
+السيدات اللي ومات يروحوا على واحدة مصابة وواحد
+
+640
+00:49:16,390 --> 00:49:20,570
+مصاب ياخدوا منه ويحقنوا بناتهم في مناطق .. في
+
+641
+00:49:20,570 --> 00:49:25,670
+مناطق من الدسم غير .. يغير الوجهة فكان عمال يتجلى
+
+642
+00:49:25,670 --> 00:49:29,250
+انتصاب بيها مرة واحدة وابتدا الحفرة في المكان اللي
+
+643
+00:49:29,250 --> 00:49:33,730
+يبين عنه غير ظاهر لكن تضمن انه مايصيبش الوجهة وهذه
+
+644
+00:49:33,730 --> 00:49:37,520
+كانت برضه من اللي هو عملية ال vaccinationاللي
+
+645
+00:49:37,520 --> 00:49:43,540
+أُخدت بالـ Experience هذا
+
+646
+00:49:43,540 --> 00:49:48,860
+الـ A. Louis Pasteur تميز
+
+647
+00:49:48,860 --> 00:49:54,320
+عشان أميزكم بين العلماء
+
+648
+00:49:54,320 --> 00:50:01,840
+كان رأسه مزدحم بالأفكار بمعنى أنه كان يقترح و عنده
+
+649
+00:50:01,840 --> 00:50:06,460
+أفكار يقوله ألف فكرة في الشهر تصبح منها فكرة واحدة
+
+650
+00:50:06,460 --> 00:50:17,920
+من الألفبعكس أزميله Robert Koch Robert
+
+651
+00:50:17,920 --> 00:50:25,400
+Koch كان hard-headed عنيد يعني لا يقفنق بسهولة
+
+652
+00:50:25,400 --> 00:50:30,300
+تقرا ورا حده بضل وجهه وراه لم يخلصها وكان من نتائج
+
+653
+00:50:30,300 --> 00:50:33,500
+أعماله كنا فرضية Koch اللي هتطلقنا إن شاء الله
+
+654
+00:50:33,500 --> 00:50:37,060
+المحاضرة اللي جاية وهي فرضية رائعة جدامن خلالها
+
+655
+00:50:37,060 --> 00:50:40,800
+أثبت أن هذا الميكروب هو المسبب لهذا المرض انا
+
+656
+00:50:40,800 --> 00:50:43,920
+أعتقد مرض عليه ممكن فيه حاجة او شيء فهنطلق لها ان
+
+657
+00:50:43,920 --> 00:50:52,540
+شاء الله في
+
+658
+00:50:52,540 --> 00:50:57,040
+عالم اسمه Dimitry Ivanovsky هو عالم روسي هو اللي
+
+659
+00:50:57,040 --> 00:51:01,300
+اكتشف الفيروسات و اكتشاف الفيروسات كان بطريقة يعني
+
+660
+00:51:01,300 --> 00:51:05,040
+فيها نوع من المصادقة انه كان بيحاول ان ي filter
+
+661
+00:51:05,480 --> 00:51:11,180
+العمل المسبب لمرض تبرقش الدخان، الدخان اللي هو ال
+
+662
+00:51:11,180 --> 00:51:16,020
+tobacco، tobacco فكان
+
+663
+00:51:16,020 --> 00:51:19,400
+يميل عبر المرشحات البكتيرية و ياخد السائل و يروشه
+
+664
+00:51:19,400 --> 00:51:22,500
+على النباتي، عوض النباتي الصعب فجال إن العامل
+
+665
+00:51:22,500 --> 00:51:28,360
+المسبب لتبرقش أوراق ال tobacco هو filterable،
+
+666
+00:51:28,360 --> 00:51:31,400
+filterable يعني قارب .. يعني بدخل من .. يعني أنا
+
+667
+00:51:31,400 --> 00:51:35,210
+حاجة وزرمةوبعدين اهيب هو ان هو المرة جاى ان شاء
+
+668
+00:51:35,210 --> 00:51:38,350
+الله نكمل مقدماتنا ونبدأ بمحاضرة اول ان شاء الله
+
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/m85QUrbJGqM_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/m85QUrbJGqM_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..76ad70efdaff4e5dfa09e3e28a31979fed9cc993
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/m85QUrbJGqM_raw.json
@@ -0,0 +1 @@
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[{"start": 3092.73, "end": 3093.17, "word": "وبعدين", "probability": 0.6485188802083334}, {"start": 3093.17, "end": 3093.41, "word": " اهيب", "probability": 0.3338623046875}, {"start": 3093.41, "end": 3093.53, "word": " هو", "probability": 0.30078125}, {"start": 3093.53, "end": 3093.73, "word": " ان", "probability": 0.439208984375}, {"start": 3093.73, "end": 3094.07, "word": " هو", "probability": 0.84228515625}, {"start": 3094.07, "end": 3094.85, "word": " المرة", "probability": 0.6357421875}, {"start": 3094.85, "end": 3095.01, "word": " جاى", "probability": 0.6533203125}, {"start": 3095.01, "end": 3095.05, "word": " ان", "probability": 0.50732421875}, {"start": 3095.05, "end": 3095.21, "word": " شاء", "probability": 0.944580078125}, {"start": 3095.21, "end": 3095.43, "word": " الله", "probability": 0.93896484375}, {"start": 3095.43, "end": 3096.05, "word": " نكمل", "probability": 0.9884440104166666}, {"start": 3096.05, "end": 3096.69, "word": " مقدماتنا", "probability": 0.92431640625}, {"start": 3096.69, "end": 3097.19, "word": " ونبدأ", "probability": 0.80682373046875}, {"start": 3097.19, "end": 3097.77, "word": " بمحاضرة", "probability": 0.87880859375}, {"start": 3097.77, "end": 3097.93, "word": " اول", "probability": 0.820556640625}, {"start": 3097.93, "end": 3098.03, "word": " ان", "probability": 0.89453125}, {"start": 3098.03, "end": 3098.25, "word": " شاء", "probability": 0.989501953125}, {"start": 3098.25, "end": 3098.35, "word": " الله", "probability": 0.8759765625}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 3099.72475, "duration_after_vad": 3022.296874999986}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/m_if8j49Njk_postprocess.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/m_if8j49Njk_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..53c02078fe990bef08165501e994f25db140b338
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/m_if8j49Njk_postprocess.srt
@@ -0,0 +1,2388 @@
+1
+00:00:20,210 --> 00:00:24,670
+بسم الله الرحمن الرحيم المحاضرة السابقة بدأنا
+
+2
+00:00:24,670 --> 00:00:29,770
+بالحديث عن ال Vibrio و اليوم هتكلم عن مجموعة اسمها
+
+3
+00:00:29,770 --> 00:00:30,230
+Sodomona
+
+4
+00:00:35,180 --> 00:00:42,340
+سودامونس نفسها و المجموعة اللى تنتمى إلها لا يربط
+
+5
+00:00:42,340 --> 00:00:48,360
+بينها يعنى كتير من الصفات لكن عنوان أساسى إنها non
+
+6
+00:00:48,360 --> 00:00:52,560
+-fermenter بمعنى إنهم كلهم بيخمروش لجلوكوز غير
+
+7
+00:00:52,560 --> 00:00:54,180
+قادر على تخمير الجلوكوز
+
+8
+00:01:05,810 --> 00:01:10,310
+وراق لجلة، وراق لجلة، الفرق بين الاتنين هنا أنه
+
+9
+00:01:10,310 --> 00:01:13,550
+بلد ما هي curved، البس الله هنا ايه؟ straight،
+
+10
+00:01:13,550 --> 00:01:17,630
+يعني مافيش فيها الانحلاق اللي موجود في ال .. ال
+
+11
+00:01:17,630 --> 00:01:21,760
+vibeالgeneral characteristics لهذه المجموعة أنها
+
+12
+00:01:21,760 --> 00:01:25,160
+opportunistic pathogens of plants, animals and
+
+13
+00:01:25,160 --> 00:01:28,720
+humans بينما لما تخلقنا على الانترو بكتيرياسي ما
+
+14
+00:01:28,720 --> 00:01:32,400
+ذكرنا ال plants، ال animals لأ، في أشياء مشترك،
+
+15
+00:01:32,400 --> 00:01:35,980
+وخدوا بالكوا من كلمة مشترك، تسمعوا بمصطلح zoonotic
+
+16
+00:01:35,980 --> 00:01:40,160
+diseases، zoonotic diseases هي الأونات المشتركة،
+
+17
+00:01:40,160 --> 00:01:46,040
+بتصيب الحيوانأو العكس بتصيب الإنسان و بتنتقل كمان
+
+18
+00:01:46,040 --> 00:01:49,060
+لمين؟ للحيوان، يعني في صنفين، في منتقل من الحيوان
+
+19
+00:01:49,060 --> 00:01:53,200
+للإنسان و من الإنسان للحيوان لكن في النهاية نسبة
+
+20
+00:01:53,200 --> 00:01:57,380
+تتصل إلى أكتر من 60% من الأمراض المعدية هي أمراض
+
+21
+00:01:57,380 --> 00:02:02,260
+مشتركة و لا تنسوا إن أحنا بنعيش في عالم غير بعيدين
+
+22
+00:02:02,260 --> 00:02:05,880
+عن الحيوان، كيف؟ و أحنا عايشين في مدن في بعض
+
+23
+00:02:05,880 --> 00:02:09,160
+الأحيان، في شقة تعالوا تطلعوا على إيش احتكاك
+
+24
+00:02:09,160 --> 00:02:14,540
+الإنسان بالحيوانأول احتكاك بنتكلم عنه كلكم تعرفوه
+
+25
+00:02:14,540 --> 00:02:20,700
+كغذاء صح؟ و بما أنه غذاء .. بما أنه غذاء إذا ينمى
+
+26
+00:02:20,700 --> 00:02:24,660
+و يباع في الأسواق .. شوفوا لإن ينمى يعني يستزرع مش
+
+27
+00:02:24,660 --> 00:02:29,600
+بيقولوا مزارع، يستزرع الدواجن ففي ناس بتربي دواجن،
+
+28
+00:02:29,600 --> 00:02:32,580
+في ناس بتنقل دواجن، في ناس بتبيع دواجن، في ناس
+
+29
+00:02:32,580 --> 00:02:34,900
+بتمظف دواجن أو في ناس بتاكل
+
+30
+00:02:38,240 --> 00:02:42,920
+انه فى حلقة كبيرة من السكان اللى احتكاك بال .. بال
+
+31
+00:02:42,920 --> 00:02:47,280
+.. بالحياة من ناحية غذائى نجى الان من ناحية اللى
+
+32
+00:02:47,280 --> 00:02:51,880
+هو ال .. ال bits اللى هم الحياة الأليفة البعض
+
+33
+00:02:51,880 --> 00:02:56,460
+بيقتنى قطط فى المنازل صح ولا لأ البعض بيقتنى طيور
+
+34
+00:02:56,460 --> 00:03:01,820
+لزينا عصفر ببغاء دماء وحطه دجافة وعنده فى البيت
+
+35
+00:03:01,820 --> 00:03:08,530
+وعصفور و الببغاء و القطط قد تنقل الأمراضالاحتكاك
+
+36
+00:03:08,530 --> 00:03:14,590
+التالت اللي هي القوارض المنتشرة، الفقران والجرذان
+
+37
+00:03:14,590 --> 00:03:19,410
+اللي موجودة بكثرة في بعض المناطق، قد تصل إلى يعني
+
+38
+00:03:19,410 --> 00:03:23,930
+البيوت معظم الناس، لأن هناك احتكاك قوي ما بين
+
+39
+00:03:23,930 --> 00:03:26,390
+الإنسان وما بين الحيوانات بتلت أشكال، الشكل الأول
+
+40
+00:03:26,390 --> 00:03:29,750
+بيقولنا كده مرة، اللي هو الغذاء، الشكل التاني،
+
+41
+00:03:29,750 --> 00:03:33,240
+حيالة القليفة، الشكل التالت اللي هواللي هو القوارب
+
+42
+00:03:33,240 --> 00:03:36,200
+او الحيوانات المنتشرة في مثلا الكلاب الضالة ايضا
+
+43
+00:03:36,200 --> 00:03:41,220
+كمان هي تتبر من الحيوانات المزايدة في احتكاك رابع
+
+44
+00:03:41,220 --> 00:03:44,440
+وخالم وثالث لكن لفئات معينة من الناس مثلا الطباء
+
+45
+00:03:44,440 --> 00:03:48,100
+البيطاريين اللي لهم خصية صح الناس اللي بيشتغلوا في
+
+46
+00:03:48,100 --> 00:03:53,180
+هدفة الحيوان هدولهم خصية احنا بيقولنا المربين
+
+47
+00:03:53,180 --> 00:04:00,040
+هدولة شملناهم في موضوع المزارة طيبإذن إمكانية
+
+48
+00:04:00,040 --> 00:04:03,020
+انتقال الميكروبات من الحيوان للإنسان أو من الإنسان
+
+49
+00:04:03,020 --> 00:04:06,540
+للحيوان الموجودة حتى في المجتمع المدني وإن يعني
+
+50
+00:04:06,540 --> 00:04:11,420
+رؤيتنا للحيوانات الحية قليل لكن بنتعامل معها كغدا
+
+51
+00:04:11,420 --> 00:04:15,780
+على الأقل clinically important aerobic gram
+
+52
+00:04:15,780 --> 00:04:18,660
+negative بس الله لأ كلمة aerobic محورية في
+
+53
+00:04:18,660 --> 00:04:22,940
+المحاضرة تبعتنا اليوم كلمتين محورية non-fermented
+
+54
+00:04:22,940 --> 00:04:26,810
+و non-aerobicلأنه ايش جاب ال pseudomonas
+
+55
+00:04:26,810 --> 00:04:30,810
+aeruginosa للاسنيتوباكتر؟ هذه واحدة على الطرف هذا
+
+56
+00:04:30,810 --> 00:04:34,690
+كانت في الداجلان فاكرين؟ في ال oxidase، اللي فصلهم
+
+57
+00:04:34,690 --> 00:04:38,290
+ال oxidase، لكن لو طلعنا على وين مقرهم في ال
+
+58
+00:04:38,290 --> 00:04:42,350
+glucose fermentation عليهم نفس المكان، both are
+
+59
+00:04:42,350 --> 00:04:45,170
+non-fermental، إذن الصفة الأساسية بالفعل إنهم non
+
+60
+00:04:45,170 --> 00:04:49,750
+-fermental، رقم اتنين إنهم ايروبي، وطبعا يعني أتقد
+
+61
+00:04:49,750 --> 00:04:54,230
+إن هذي بتأدي لهذه، هذي بتأدي لهذه، صح ولا بقى؟و
+
+62
+00:04:54,230 --> 00:04:56,910
+طبعا بتقدي لجنود تانية هتعرفها كمان شوية أنه لازم
+
+63
+00:04:56,910 --> 00:05:00,150
+تكون catalyse postive مثلا لازم تكون .. مش لازم
+
+64
+00:05:00,150 --> 00:05:02,410
+تكون oxalyse postive لكن catalyse لازم تكون
+
+65
+00:05:02,410 --> 00:05:06,790
+postive أسماء مهمة Sodomonas aeruginosa
+
+66
+00:05:06,790 --> 00:05:11,750
+borcholderia حلوا اسمها ده borcholderia sebacia و
+
+67
+00:05:11,750 --> 00:05:14,350
+لو درستوا قبل سنتين أو تلاتين مع الدكتور عبد
+
+68
+00:05:14,350 --> 00:05:16,270
+القادر اللي قدر كان جالكوا Sodomonas sebacia ..
+
+69
+00:05:16,270 --> 00:05:19,150
+sebacia لأن اسم القديم اللي كان Sodomonas sebacia
+
+70
+00:05:19,150 --> 00:05:23,910
+جديد سميت borcholderiaالاسم التالت طويل شوية
+
+71
+00:05:23,910 --> 00:05:28,030
+بلانجته استينوتروفوموناص
+
+72
+00:05:28,030 --> 00:05:35,330
+مالتوفيلا استينوتروفوموناص مالتوفيلا الرابع
+
+73
+00:05:35,330 --> 00:05:40,630
+أسينيتوبكتر بوماني الخامس موراجزيلا كاتيراز أسماء
+
+74
+00:05:40,630 --> 00:05:43,130
+طبعا خدوا بالكم أنا كمان مرة في ال medical
+
+75
+00:05:43,130 --> 00:05:47,990
+microbiology لا نقشت الانتروباترياس كلهم ولا نقشت
+
+76
+00:05:47,990 --> 00:05:51,650
+ال Vibrio حتى كمان كلها أنا نقشت جزئية منهاأنا
+
+77
+00:05:51,650 --> 00:05:55,750
+بحاول أستعرض أمامكوا بعض النقاط اللي هدرسهم
+
+78
+00:05:55,750 --> 00:05:59,850
+بتفاصيل أكبر في وين ال diagnostic microbiology لكن
+
+79
+00:05:59,850 --> 00:06:03,610
+بوديكموا نماذج ان كيف احنا ممكن نستفيد من ال
+
+80
+00:06:03,610 --> 00:06:05,690
+medical microbiology ل ال diagnostic microbiology
+
+81
+00:06:05,690 --> 00:06:09,530
+خدوا بالكم البعض بيقولوا ال microbiology هي course
+
+82
+00:06:09,530 --> 00:06:13,890
+حفظ لازم نحفظ المعلومة انا لا اريد ان اجادلكم في
+
+83
+00:06:13,890 --> 00:06:17,610
+هذا لا اريد ان اجادلكم في انها it requires
+
+84
+00:06:17,610 --> 00:06:24,480
+memorizationلكن ولا واحد حفظ ال microbiology فقط و
+
+85
+00:06:24,480 --> 00:06:28,340
+فلح انه يصير microbiologist لازم يكون عنده مقدرة
+
+86
+00:06:28,340 --> 00:06:34,140
+على انه يربط المعلومات اللي حفظها مع بعض و هو
+
+87
+00:06:34,140 --> 00:06:38,480
+التعريف الحقيقي لل science واحد جال التعريف العلم
+
+88
+00:06:38,480 --> 00:06:43,360
+جال it is not learning a bunch of facts ليس العلم
+
+89
+00:06:43,360 --> 00:06:48,330
+هو عملية تعلم حفنة من الحقائقناخدها من المدرس و
+
+90
+00:06:48,330 --> 00:06:53,030
+نرجع نرددلها تاني تمام؟ بنعرف هذا الحقائق ليس
+
+91
+00:06:53,030 --> 00:06:57,290
+learning a bunch of facts it is a systematic
+
+92
+00:06:57,290 --> 00:07:03,990
+process عملية نظامية بجمع الحقائق و بربطها ببعض
+
+93
+00:07:03,990 --> 00:07:09,690
+حتى أصل applied حاجة تطبيقية حاجة تطبيقية أستفيد
+
+94
+00:07:09,690 --> 00:07:13,770
+منها وبالتالي مطلوب مننا نحفظناها زي واحد بيقولك
+
+95
+00:07:13,770 --> 00:07:19,020
+انابحبش الحفظ لكن بفهم، بنقولله كيف يعني مثلا
+
+96
+00:07:19,020 --> 00:07:21,820
+أحافظ نظريات في سجول و أقولها فاهمها، بقولك أنا
+
+97
+00:07:21,820 --> 00:07:25,180
+فاهمها، طب قولنا إياها، كم بيشرحلنا إياها، طب ما
+
+98
+00:07:25,180 --> 00:07:28,880
+هو شرحك اللي هدل علينا كده، حافظها، وبتالي ال ..
+
+99
+00:07:28,880 --> 00:07:31,860
+تنتين ما بيجب أن تجزّيهم مع بعض، الحفظ مهم لعملية
+
+100
+00:07:31,860 --> 00:07:36,460
+برضه إيه الفهم، في كثير من الأحيان، و أهم من ذلك
+
+101
+00:07:36,460 --> 00:07:37,180
+دليل التحقق
+
+102
+00:07:41,870 --> 00:07:43,990
+General characteristics of non-fermenters,
+
+103
+00:07:44,230 --> 00:07:46,310
+oxidative-gram-negative bacilli including
+
+104
+00:07:46,310 --> 00:07:49,310
+pseudomonas species produces acid from glucose or
+
+105
+00:07:49,310 --> 00:07:53,550
+other carbohydrates only in the presence of oxygen
+
+106
+00:07:53,550 --> 00:07:59,310
+تعالى الأن نسأل سؤال للـ quiz الجاى ونقول true or
+
+107
+00:07:59,310 --> 00:08:04,710
+false pseudomonas aeruginosa is glucose positive
+
+108
+00:08:04,710 --> 00:08:05,910
+صحة لغلط
+
+109
+00:08:11,040 --> 00:08:14,720
+أنا قلت fermentation، قلت جلوكوز positive، لا قلت
+
+110
+00:08:14,720 --> 00:08:17,820
+fermentation ولا قلت أي حاجة تانية، قلت جلوكوز
+
+111
+00:08:17,820 --> 00:08:25,020
+positive طيب، أنا بقولكوا true، و أنا صحي و أنتوا
+
+112
+00:08:25,020 --> 00:08:30,760
+صح، إذا أنا اتفرجتها من المنظور إنه it is جلوكوز
+
+113
+00:08:30,760 --> 00:08:36,210
+oxidizer positive صحوزي انتوا قدرتولها من منظور ال
+
+114
+00:08:36,210 --> 00:08:39,690
+glucose fermentation انتوا صح طب من أي منظور من
+
+115
+00:08:39,690 --> 00:08:45,710
+منظورها انتوا معايا عندنا احتماليتين او تلت
+
+116
+00:08:45,710 --> 00:08:48,990
+احتمالات للتعامل مع ال carbohydrate بالنسبة
+
+117
+00:08:48,990 --> 00:08:52,040
+للبكتيرياشايفين الأنبوبة اللي على الصبورة اللي
+
+118
+00:08:52,040 --> 00:08:56,840
+أمامنا؟ فيها أنبوبتين زي بعض زرعناهم بنفس .. اللي
+
+119
+00:08:56,840 --> 00:08:58,960
+هو أنبوبتين أفضل نفس ال media زرعناهم بنفس
+
+120
+00:08:58,960 --> 00:09:02,460
+البكتيريا بنفس البكتيريا واحدة منهم حاطين على ال
+
+121
+00:09:02,460 --> 00:09:06,940
+top زي ما أنتوا شايفين الزيت والتانية بدون زيت،
+
+122
+00:09:06,940 --> 00:09:10,220
+الزيت مش عمل .. عمل طبقة عازلة حتى يمنع ال oxygen
+
+123
+00:09:10,220 --> 00:09:15,800
+عن ال mediaالان لو البكتيريا بكتيريا لا هوائية ..
+
+124
+00:09:15,800 --> 00:09:19,680
+لا هوائية أو أنت المقدر تعمل fermentation أكيد
+
+125
+00:09:19,680 --> 00:09:22,360
+هتستغل ال .. ال glucose اللي مورود في اليوم بوبا
+
+126
+00:09:22,360 --> 00:09:25,900
+اللي فيها زيت و تغير لون الوصلة صح ولا لأ؟ يبقى
+
+127
+00:09:25,900 --> 00:09:28,700
+هيك بتكون أشياء fermented .. ف .. خلانا نقول
+
+128
+00:09:28,700 --> 00:09:31,460
+fermented .. بدنا .. بدنا أصلا كلمة بستر و نجد
+
+129
+00:09:31,460 --> 00:09:34,960
+بدنا نغير رفوم اليومفي الموضوع التاني، لو
+
+130
+00:09:34,960 --> 00:09:38,140
+البكتيريا غيرت اللون، وإذا ملاحظين أنه غير اللون،
+
+131
+00:09:38,140 --> 00:09:41,420
+وين مغير اللون؟ على السطح، دلوقت أنا أبدأها إيه؟
+
+132
+00:09:41,420 --> 00:09:46,780
+إيه الهواء، يفجأة هي عملت oxidation للكربوهيدرات
+
+133
+00:09:46,780 --> 00:09:48,960
+هناك، واحدة عملت oxidation، واحدة عملت
+
+134
+00:09:48,960 --> 00:09:52,860
+fermentation، لو عملت في اتنين، بتكون oxidative،
+
+135
+00:09:52,860 --> 00:09:56,700
+fermentative، يعني البكتيريا facultative، أنا ايضا
+
+136
+00:09:56,970 --> 00:10:00,950
+لو عملت فقط في الهوائية فهي oxidative لو عملت في
+
+137
+00:10:00,950 --> 00:10:03,970
+اللي فيها زيت بتكون أنا aerobic و بتعمل
+
+138
+00:10:03,970 --> 00:10:07,310
+fermentation إذا الأن كيف بدنا نفسر نتائج طبعتنا
+
+139
+00:10:07,310 --> 00:10:11,290
+لما نقول جليكوز positive ولا جليكوز negative لازم
+
+140
+00:10:11,290 --> 00:10:14,270
+نكون عارفين السؤال بتكلم عن oxidation ولا عن مين
+
+141
+00:10:14,270 --> 00:10:21,090
+عن fermentation طيب في حل تالت الحين البكتيريا لما
+
+142
+00:10:21,090 --> 00:10:25,730
+أكثرت الجليكوز إيش يعني أكثرته استغلته؟لما عملتله
+
+143
+00:10:25,730 --> 00:10:29,330
+fermentation ايش يعني؟ يعني استغلته في مصطلح اسمه
+
+144
+00:10:29,330 --> 00:10:32,790
+glucose utilization او carbohydrate utilization ال
+
+145
+00:10:32,790 --> 00:10:37,770
+utilization انا مش فارقة عندي هل هو aerobic ولا
+
+146
+00:10:37,770 --> 00:10:41,630
+انا aerobic اللي هو من قدرة ايه؟ تستغله فإذا قلت
+
+147
+00:10:41,630 --> 00:10:46,030
+glucose utilization فبالفعل ال enterobacteriasi
+
+148
+00:10:46,030 --> 00:10:50,290
+بسطه و ال salomonas برضه بسطه لكن لو قلت
+
+149
+00:10:50,290 --> 00:10:55,300
+fermentationالإنتيرو بكتيرياسي positive بينما ال
+
+150
+00:10:55,300 --> 00:10:57,840
+pseudomonas يبدأ بدل مصطلحات يكون إيش اللي أقل
+
+151
+00:10:57,840 --> 00:11:00,880
+عنها معروفة تلتة إيش هم اللي أقل عنها تلتة؟
+
+152
+00:11:00,880 --> 00:11:04,940
+Fermentative, Oxidative و Utilization كلمة
+
+153
+00:11:04,940 --> 00:11:08,120
+Utilization فينك مرة مش فارقة معناها إنه هيك ولا
+
+154
+00:11:08,120 --> 00:11:13,560
+هيك في كمان احتبار راح بعملش بنكبر محنا كل شوية كل
+
+155
+00:11:13,560 --> 00:11:19,000
+خمس دقايق بنكبر خمس دقايق الآن عندي بكتير اللي
+
+156
+00:11:19,000 --> 00:11:22,540
+عندها القدرة إنها تعمل oxidationولا fermentation
+
+157
+00:11:22,540 --> 00:11:26,560
+بيسموها Asacarolithica Asacarolithica يبقى
+
+158
+00:11:26,560 --> 00:11:29,600
+Utilization, Oxidation, Fermentation و
+
+159
+00:11:29,600 --> 00:11:34,360
+Asacarolithicus يعني غير قادرة على استغلال زي آخر
+
+160
+00:11:34,360 --> 00:11:37,840
+واحدة على الصبورة عنها اسمها Alkalogenes fecalis
+
+161
+00:11:37,840 --> 00:11:41,300
+neither ferments nor oxidizes الجلوكوز اللي هو
+
+162
+00:11:41,300 --> 00:11:43,940
+بتخمر ولا بتاكسد اللي هو الجلوكوز بينما
+
+163
+00:11:43,940 --> 00:11:47,540
+السودومولاس كريجنوزا اللي جابلها it oxidizes but
+
+164
+00:11:47,540 --> 00:11:48,440
+does not ferment
+
+165
+00:11:54,010 --> 00:11:57,790
+مش محطة محترمة مش carbon neutralizer كاربوهايدريت
+
+166
+00:11:57,790 --> 00:12:01,910
+لأنه .. لأنه .. لأنه بدأ مصدر كربوني و هتحتاري
+
+167
+00:12:01,910 --> 00:12:06,150
+المصدر الكربوني من مصادر أخرى مثلا من ال proteins
+
+168
+00:12:06,150 --> 00:12:11,830
+أو من ال .. ال lipids أو من ال nucleic acids فاهم؟
+
+169
+00:12:11,830 --> 00:12:15,770
+يبقى متفقين الآن على أربع مصطلحات argue أنه الأمور
+
+170
+00:12:15,770 --> 00:12:19,150
+تكون واضحة بنفسنا دخلين في توضيح الأمور خلينا نوضح
+
+171
+00:12:19,150 --> 00:12:23,880
+كمان أمر تانيالان لما انا بقول glucose positive او
+
+172
+00:12:23,880 --> 00:12:26,400
+glucose negative او اي صفة من الصفات اللي درستوها
+
+173
+00:12:26,400 --> 00:12:28,600
+في المعمل من ال indole من ال methyl red من الفوكرس
+
+174
+00:12:28,600 --> 00:12:32,180
+بروسكاوه انتوا بتدرسوكوا ايها absolute بيقولك ال E
+
+175
+00:12:32,180 --> 00:12:34,660
+coli positive positive negative negative ال
+
+176
+00:12:34,660 --> 00:12:37,040
+enterobacter negative negative positive positive
+
+177
+00:12:37,040 --> 00:12:40,360
+بصبوط لل invic ده ال indole من ال methyl red من
+
+178
+00:12:40,360 --> 00:12:42,400
+الفوكرس بروسكاوه او ال citrate انا الان بده
+
+179
+00:12:42,400 --> 00:12:47,000
+افهمكوا مفهوم تاني مفهوم تشكيكي خالص تمام؟ مش
+
+180
+00:12:47,000 --> 00:12:51,840
+هنوظفه الآن لكن غصب عنه احنا هحتاجهلو جيبنا 100
+
+181
+00:12:51,840 --> 00:12:55,380
+pseudomonas aeruginosa مائة species معرفين علي
+
+182
+00:12:55,380 --> 00:12:58,480
+انهم pseudomonas aeruginosa وسألناهم سؤال هل انتي
+
+183
+00:12:58,480 --> 00:13:04,540
+اكسيدايز بوستف المية اللي جينا منهم 97 بوستف و 3%
+
+184
+00:13:04,540 --> 00:13:08,640
+منهم negative تلاتة من المية negative ايه أوصف
+
+185
+00:13:08,640 --> 00:13:12,850
+الصفة؟بتقول ال post-it؟ إلكوا بقول post-it طبعا؟
+
+186
+00:13:12,850 --> 00:13:15,750
+طب ده ال computer مش فيه إمكانيه إنه أنا أتعامل
+
+187
+00:13:15,750 --> 00:13:17,930
+معه أنا مش حاجة ده انا اصلا انا .. انا ملاقى
+
+188
+00:13:17,930 --> 00:13:21,450
+الصعوبة أحفظ ال post-it و ال negative صح؟ فكمان
+
+189
+00:13:21,450 --> 00:13:23,550
+أحفظ ال post-it و ال negative و جدّين ال
+
+190
+00:13:23,550 --> 00:13:26,170
+percentage ده بتقول إن ال post-it أو ال negative
+
+191
+00:13:26,170 --> 00:13:30,740
+هذا هيكون أمر صعب جدالا يقيقوا إلا قواعد البيانات
+
+192
+00:13:30,740 --> 00:13:34,240
+طبعا الحصول، لذلك البنات اللي نزلوا البرنامج اللي
+
+193
+00:13:34,240 --> 00:13:39,020
+اسمه IDBAC لو طلّوا على المصففات، هيلجوا أسماء
+
+194
+00:13:39,020 --> 00:13:43,240
+البكتيريا و أسماء الفحوصاتو مش مكتوب positive و
+
+195
+00:13:43,240 --> 00:13:48,780
+negative مكتوب percentage 97% , 99% , 100% , 50%
+
+196
+00:13:48,780 --> 00:13:51,240
+يعني في بعض الصفات قد تصل الى fifty و هذه اللي ..
+
+197
+00:13:51,240 --> 00:13:54,840
+لما تصل الى fifty بتسميها variable يعني هو ممكن
+
+198
+00:13:54,840 --> 00:13:58,520
+positive و ممكن negative أقل من fifty بتميل إلى
+
+199
+00:13:58,520 --> 00:14:01,440
+هتكون negative أكتر من fifty بتتميل إلى نقول أنها
+
+200
+00:14:01,440 --> 00:14:06,240
+positive لكن في النهاية يعني كثير من الصفات مش
+
+201
+00:14:06,240 --> 00:14:08,240
+absolute مش one hundred percent هذا اللي بدنا
+
+202
+00:14:08,240 --> 00:14:12,990
+نوصلكوا إلى كافة كلمةطيب Sodomeras aeruginosa
+
+203
+00:14:12,990 --> 00:14:19,330
+الجميلة ذات الرائحة الذكية و في نفس الوقت سيئة
+
+204
+00:14:19,330 --> 00:14:24,210
+السمعة سيئة السمعة لإنه it causes hard to treat
+
+205
+00:14:24,210 --> 00:14:29,030
+infections بتسبب أمراض صعب الشفاء منها حتى
+
+206
+00:14:29,030 --> 00:14:34,650
+باستخدام ال antibiotics جميلة لإن ألوانها براقة و
+
+207
+00:14:34,650 --> 00:14:37,330
+دراستوها انتوا في ال pigments لما كنا سليبوا ال
+
+208
+00:14:37,330 --> 00:14:39,730
+pigments blue green pigments blue green و الاخر
+
+209
+00:14:39,730 --> 00:14:44,000
+حمره بعناو جميلة لإنه بتنتج زي ما شايفينها احنا في
+
+210
+00:14:44,000 --> 00:14:49,580
+احد الأماكن هيتها grape-like odor اللي هي رائحة
+
+211
+00:14:49,580 --> 00:14:53,440
+تشبه رائحة العنب او بعض الأحيان يعني انا لعشرين
+
+212
+00:14:53,440 --> 00:14:57,160
+سنة بشم سودا مونس ماعمر شمت رائحة العنب بشم رائحة
+
+213
+00:14:57,160 --> 00:15:03,340
+جميلة fruity odor بسمها fruity odor إذن لون حلو و
+
+214
+00:15:03,340 --> 00:15:08,540
+رائحة جميلة تمام إلا إنه بتسبب hard to treat ..
+
+215
+00:15:08,540 --> 00:15:13,140
+hard to treat infectionsو الحقيقة انه حتى قبل
+
+216
+00:15:13,140 --> 00:15:17,460
+اكتشاف ال antibiotic البكتيريا معروفة بطبيعيها
+
+217
+00:15:17,460 --> 00:15:22,520
+مقاومة مش اكتسبت مقاومة هي بطبيعيها يعني من أول ما
+
+218
+00:15:22,520 --> 00:15:24,620
+اكتشفنا ال antibiotic هما بنعرف ان ال pseudomorous
+
+219
+00:15:24,620 --> 00:15:27,540
+ايه؟ resistant لل antibiotic natural resistant
+
+220
+00:15:27,540 --> 00:15:31,940
+نسموه غير ال natural resistance عندها المقدرة to
+
+221
+00:15:31,940 --> 00:15:35,920
+acquire resistance كمان يعني انا قبل فترة عرض
+
+222
+00:15:35,920 --> 00:15:40,610
+presentation في احد المؤتمراتووصلت نسبة ال
+
+223
+00:15:40,610 --> 00:15:43,850
+resistance لبعض ال antipatetic hormones 100% بمعنى
+
+224
+00:15:43,850 --> 00:15:47,650
+أن ولا عزلة اللي عزلناهم كانت sensitive لل
+
+225
+00:15:47,650 --> 00:15:50,270
+antipatetic اللي أصل هي sensitive لها يعني معناها
+
+226
+00:15:50,270 --> 00:15:53,010
+عخر عندها natural resistance و عندها acquired
+
+227
+00:15:53,010 --> 00:15:57,730
+resistance طيب المعنى المروسة يعنيالجملة الأولى
+
+228
+00:15:57,730 --> 00:16:04,670
+disregard كلمة group multiple هي single فهنشيل
+
+229
+00:16:04,670 --> 00:16:08,250
+الحق يعني من ال presentation فقط هنخلي single هو
+
+230
+00:16:08,250 --> 00:16:12,090
+الرفلجلة هنشيل كلمة هو multiple gram negative بس
+
+231
+00:16:12,090 --> 00:16:15,510
+الله it is obligate erupts طبعا obligate يعني
+
+232
+00:16:15,510 --> 00:16:19,150
+strict يعني مابتقدرش تناميها لها وقت طبعا بقولها
+
+233
+00:16:19,150 --> 00:16:22,350
+most strange كأي صفة من الصفات اللي قولنا عنها مش
+
+234
+00:16:22,350 --> 00:16:27,530
+absoluteOxidized usually and catalysed positively
+
+235
+00:16:27,530 --> 00:16:29,850
+هاي حتى ال Oxidized الصفة اللي احنا ميزنا فيها صدر
+
+236
+00:16:29,850 --> 00:16:33,930
+موارث على جهة بيقولنا مش مش مش مش absolute مش
+
+237
+00:16:33,930 --> 00:16:38,170
+absolute البعض الصليحات منها بتكون negative Non
+
+238
+00:16:38,170 --> 00:16:41,550
+-fermentative chemoheterotrophic respiratory
+
+239
+00:16:41,550 --> 00:16:46,070
+metabolism minimum nutritional requirements بمعنى
+
+240
+00:16:46,070 --> 00:16:50,830
+انهتستطيع إنها تعيش على أقل القيادة و أنا ممكن
+
+241
+00:16:50,830 --> 00:16:54,090
+قلتلكوا مرة على ال bottled water أو ال .. ال
+
+242
+00:16:54,090 --> 00:16:57,970
+mineral water اللي بنشتريه، بنشربه، إيش فيه مواد
+
+243
+00:16:57,970 --> 00:17:00,370
+غذائية ده؟ ال mineral water؟ ولا إيش فيه مواد
+
+244
+00:17:00,370 --> 00:17:03,130
+غذائية؟ مواد .. مواد بسيطة جدا، بال .. بال nano،
+
+245
+00:17:03,130 --> 00:17:07,330
+بال microgram ممكن تكون، بال ppm بيجسوها، طبعا؟
+
+246
+00:17:07,330 --> 00:17:11,650
+ومع ذلك البكتيريا عندها المقدرة to thrive and ..
+
+247
+00:17:11,650 --> 00:17:14,670
+thrive and grow .. even grow in .. in this mineral
+
+248
+00:17:14,670 --> 00:17:20,350
+يعني they requirevery low nutritional requirements
+
+249
+00:17:20,350 --> 00:17:25,850
+بتحكيلكوا عن صدوموناس حد يعني بتخليكوا ذاكرين
+
+250
+00:17:25,850 --> 00:17:33,770
+الصدوموناس كويس بتعرفوا في روسيا سيبيريا طول السنة
+
+251
+00:17:33,770 --> 00:17:37,010
+تقريبا تلج هم عايشين في تلاجة أصلا في الفريزر
+
+252
+00:17:37,010 --> 00:17:40,210
+عايشين هم مش حتى في تلاجة في الفريزر فتخيلوا عند
+
+253
+00:17:40,210 --> 00:17:43,290
+واحد عنده سيارة السيارة في مي بيحطوها في الراديات
+
+254
+00:17:43,290 --> 00:17:48,010
+لإنها تبرد المحرك فتجمل الميفبروح بيحطولها
+
+255
+00:17:48,010 --> 00:17:52,950
+antifreeze .. antifreeze يعني مواد مضادة للتجمد من
+
+256
+00:17:52,950 --> 00:17:55,170
+ضمن المواد اللي .. اللي .. اللي اكتشفوها حطوها ..
+
+257
+00:17:55,170 --> 00:17:57,690
+حطولها Sodomonas Reginosa او من الأحلى Sodomonas
+
+258
+00:17:57,690 --> 00:18:01,350
+Sodomonas Reginosa ف Sodomonas لما حاطينها مع ال
+
+259
+00:18:01,350 --> 00:18:05,650
+.. مع الميه بطلت تتجمد طيب هذه نقطة تذكرها كويسة
+
+260
+00:18:05,650 --> 00:18:09,050
+انه منعانة التجمد في أحد السنوات أحد الدول اللي
+
+261
+00:18:09,050 --> 00:18:12,450
+صدفت الألعاب الأولومبية الشتوية اللي فيها تزولوا
+
+262
+00:18:12,450 --> 00:18:16,150
+العجليات أساسي المفروض ان دولة يكون مش .. مش .. مش
+
+263
+00:18:16,150 --> 00:18:19,680
+سعودية أكيدأنا صوت اللى بيكون الدولة يكون فيها
+
+264
+00:18:19,680 --> 00:18:25,380
+تلج، سنة الاستضافة صارنا المشكلة الثلج ما تحول
+
+265
+00:18:25,380 --> 00:18:29,960
+لجليد وبالتالي هتفشل ألعاب دول أمبريكية، اتصلوا في
+
+266
+00:18:29,960 --> 00:18:32,420
+ال biotechnology groups، جالنا في بيتنا، جالنا نحط
+
+267
+00:18:32,420 --> 00:18:37,060
+صدومورس، فخايل الأول مرة مانعت .. المرة حاطينة
+
+268
+00:18:37,060 --> 00:18:41,500
+عاملة .. حوّلت التلج إلى جليدلما انا بدي عزل ال
+
+269
+00:18:41,500 --> 00:18:47,260
+micro غريب الصفات بمعنى بيأكل او بيكسر مركبات
+
+270
+00:18:47,260 --> 00:18:51,460
+غريبة لما غالبا بدل العالمين على ال Sodamores
+
+271
+00:18:51,460 --> 00:18:57,660
+عندها wide range of metabolismيعني خلّينا نقول
+
+272
+00:18:57,660 --> 00:19:03,120
+مصنع .. مصنع لل enzymes مصنع كبير لل enzymes عندها
+
+273
+00:19:03,120 --> 00:19:07,060
+مقدرة مهولة على استغلال الأشياء بتخليش إشي اللي لا
+
+274
+00:19:07,060 --> 00:19:09,600
+تقدر تستغل و بالتالي pseudomonas بالفعل كان
+
+275
+00:19:09,600 --> 00:19:12,220
+survive for long الشيء السيء اللي بيقوله عن
+
+276
+00:19:12,220 --> 00:19:15,260
+pseudomonas احنا كنا زمان أنه لما نروح المستشفيات
+
+277
+00:19:15,260 --> 00:19:18,200
+عشان نرفع من ملويات مريضنا ناخدله bouquet ورد،
+
+278
+00:19:18,200 --> 00:19:25,570
+مظبوط؟ جميل، حلو، لكن من الأقل طالعلأنه هي normal
+
+279
+00:19:25,570 --> 00:19:30,510
+.. normal microbiota على ال plants فواقعي اعمل ..
+
+280
+00:19:30,510 --> 00:19:32,930
+لو بناخدله ورد احنا المريض نوديله هنا to the
+
+281
+00:19:32,930 --> 00:19:37,450
+monocery خاصة لو يكون مريض حروق او يكون مريض جروح
+
+282
+00:19:37,450 --> 00:19:41,130
+يعني عمليات او كده هذا بتأثر جدا بال .. و طبعا هي
+
+283
+00:19:41,130 --> 00:19:44,770
+بتعمل opportunistic infection اكتر ناس بيداجوا من
+
+284
+00:19:44,770 --> 00:19:48,170
+البكتيريا هم اقسام الحروق و اقسام العمليات
+
+285
+00:19:48,170 --> 00:19:50,570
+الجروحية لان بتعمل wound and burn
+
+286
+00:19:54,900 --> 00:19:58,920
+بكون إيش هو القاتل للمريض بقدر ما بتكون ال burn
+
+287
+00:19:58,920 --> 00:20:02,320
+infection هي المسئولة عن .. و طبعا ال burn
+
+288
+00:20:02,320 --> 00:20:04,920
+infection number one cause لها pseudomonas
+
+289
+00:20:04,920 --> 00:20:07,940
+erogenous و عملنا دراسة و نشرتها السنة الماضية أو
+
+290
+00:20:07,940 --> 00:20:10,860
+اللي قبلها مش فاكر بالضبط .. نشرتها في جورنال
+
+291
+00:20:10,860 --> 00:20:14,280
+أجنبي بيتكلم على ان number one cause of burn
+
+292
+00:20:14,280 --> 00:20:16,640
+infection في مستشفى الشيفة كان هو pseudomonas
+
+293
+00:20:16,640 --> 00:20:20,500
+erogenous و لجناها في دين المرضى و لجناها على فضل
+
+294
+00:20:20,500 --> 00:20:23,320
+دين ال health care workers و على الأسطح في كل مكان
+
+295
+00:20:23,320 --> 00:20:26,010
+في المستشفىفي عيادة اللي هو لجيناها كمان Sodium
+
+296
+00:20:26,010 --> 00:20:30,950
+monoxidane مثلا فقيت الشيء في الموضوع أنه ال
+
+297
+00:20:30,950 --> 00:20:34,150
+minimal nutritional requirements بتخليها to
+
+298
+00:20:34,150 --> 00:20:37,390
+survive in various environments in harsh
+
+299
+00:20:37,390 --> 00:20:41,270
+conditions مش زي البكيريا اللي لو تركناها دي اكتين
+
+300
+00:20:41,270 --> 00:20:45,150
+برا بتزعل و تموت يعني لأ هذه عندها المقدرة على
+
+301
+00:20:45,150 --> 00:20:50,710
+أنها تعيش في البيئة بشكل كبيرmany organic
+
+302
+00:20:50,710 --> 00:20:53,310
+compounds used as carbon and nitrogen sources, but
+
+303
+00:20:53,310 --> 00:20:56,690
+only few carbohydrates by oxidative metabolism،
+
+304
+00:20:56,690 --> 00:20:59,710
+الجلوكوز used oxidatively، هي وسط الجلوكوز من
+
+305
+00:20:59,710 --> 00:21:02,350
+ناحية اللي هو ال Oxidation، هذا النقطة اللي بدنا
+
+306
+00:21:02,350 --> 00:21:06,230
+نركز عليها كمان، lactose، negative، on مكونكي
+
+307
+00:21:06,230 --> 00:21:11,250
+أجانب، lactose negative يعني non-lactose fermenter
+
+308
+00:21:11,630 --> 00:21:16,290
+يعني شكل المقنكي هيكون زي شكل السلمونيلا زي شكل
+
+309
+00:21:16,290 --> 00:21:20,830
+الشجلة زي شكل البروتياز زي اليرسينيا هدولة من وين
+
+310
+00:21:20,830 --> 00:21:25,250
+هدولة من الاسماء من ال enterobacteriasis يبقى ال
+
+311
+00:21:25,250 --> 00:21:27,750
+Sodomonas و ال enterobacteriasis النالكتوزفرمنتر
+
+312
+00:21:27,750 --> 00:21:33,110
+سيبدو كأنهم شكل نوع واحد يعني النالكتوزفرمنتر
+
+313
+00:21:33,110 --> 00:21:35,910
+فبنعرف أن مش كل إشي بينمو المقنكي هو
+
+314
+00:21:35,910 --> 00:21:40,980
+enterobacteriasis قد يكون Sodomonas و أخواتهاومنها
+
+315
+00:21:40,980 --> 00:21:44,340
+الاسينيتوباكتر اللي هو non-lactose fermenter بس
+
+316
+00:21:44,340 --> 00:21:47,720
+الفرق بين ال enterobacteres اللي ذكرتهم و بين ال
+
+317
+00:21:47,720 --> 00:21:51,620
+pseudomonas ال triple sugar iron agar ايش هيطلع ال
+
+318
+00:21:51,620 --> 00:21:56,380
+triple sugar iron agar تبع ال pseudomonas؟
+
+319
+00:21:56,380 --> 00:21:58,600
+triple sugar iron agar تبع ال pseudomonas ايش
+
+320
+00:21:58,600 --> 00:22:04,560
+هيطلع؟
+
+321
+00:22:04,560 --> 00:22:07,660
+ايش
+
+322
+00:22:07,660 --> 00:22:11,280
+هيطلع النتائج النهائية المهمة؟اللون طبعا هو مش
+
+323
+00:22:11,280 --> 00:22:16,920
+هيكون أخضر،
+
+324
+00:22:16,920 --> 00:22:24,260
+هو مش مش من اللوحة ده، هيطلع
+
+325
+00:22:24,260 --> 00:22:28,350
+أحمر على أحمرلأن لو هتعمل fermentation للجليكوز و
+
+326
+00:22:28,350 --> 00:22:31,810
+لو هتعمل fermentation للاكتوز ولا للساكروزا
+
+327
+00:22:31,810 --> 00:22:35,610
+وبالتالي مش هيتغير اللون فهي هتكون K على K زي ما
+
+328
+00:22:35,610 --> 00:22:40,850
+هي عمليها هتتغير بينما لو كانت سالمونيلا هتكون K
+
+329
+00:22:40,850 --> 00:22:44,150
+على A لو كانت البروتيز هتكون K على A لو كانت
+
+330
+00:22:44,150 --> 00:22:47,010
+الشيلة هتكون K على A لأن كل ال انترو بكتيرياز
+
+331
+00:22:47,010 --> 00:22:50,950
+الجليكوز فرمنتها بينما اللي ذكرناه دول نار لاكتوز
+
+332
+00:22:50,950 --> 00:22:51,410
+فرمنتها
+
+333
+00:22:54,760 --> 00:22:58,480
+طيب some strains produces pigment او diffusable و
+
+334
+00:22:58,480 --> 00:23:02,300
+هي soluble pigment زى مثلا اللى هو ال biocyanine و
+
+335
+00:23:02,300 --> 00:23:04,640
+هذا هو ال blue pigment و ال fluorescein اللى هو ال
+
+336
+00:23:04,640 --> 00:23:07,040
+yellow pigment اذا two pigments معروفين ل ال
+
+337
+00:23:07,040 --> 00:23:10,800
+pseudomonas اللى هم ال biocyanine و هي زرقاء و ال
+
+338
+00:23:10,800 --> 00:23:15,000
+fluorescein بتكون yellowسيد موراس إرجنوزا يقول
+
+339
+00:23:15,000 --> 00:23:18,300
+إنها produces grape-like odor أو اللي سميناها
+
+340
+00:23:18,300 --> 00:23:22,680
+fruity odor and blue-green pus and cornice. إيش عن
+
+341
+00:23:22,680 --> 00:23:26,740
+ال blue-green pus؟ الصديد اللي يمكن يهانتش من هذه
+
+342
+00:23:26,740 --> 00:23:30,940
+البكتيريا برضه بيكون ماخد لون اللي هو البكتيريا
+
+343
+00:23:32,060 --> 00:23:35,960
+الدُولة الأخيرة طبعاً أهميتها أكد عليها في بيت
+
+344
+00:23:35,960 --> 00:23:39,460
+المحاضرة أنه broad spectrum .. broad antibiotic
+
+345
+00:23:39,460 --> 00:23:43,860
+resistance و بنوصفها بإنها MDR أو multiple drug
+
+346
+00:23:43,860 --> 00:23:48,480
+resistance شوفوا
+
+347
+00:23:48,480 --> 00:23:51,720
+عليك، في عندي قائمة من الأمراض الآن اللي موجودة
+
+348
+00:23:51,720 --> 00:23:55,200
+على الصبورة، صح؟اللي هو تحت أنواريش مكتوبين
+
+349
+00:23:55,200 --> 00:24:00,540
+opportunistic nosocomial infections يعني غالبا
+
+350
+00:24:00,540 --> 00:24:06,320
+hospital acquired و كمان مرة opportunistic هذه
+
+351
+00:24:06,320 --> 00:24:10,380
+ليست قائمة extensive هذه sample من الأمراض اللي
+
+352
+00:24:10,380 --> 00:24:13,060
+بتسببها ال pseudomonas aeruginosa
+
+353
+00:24:18,910 --> 00:24:21,970
+burn and wound infection قلنا لكوا انها number one
+
+354
+00:24:21,970 --> 00:24:24,530
+cause of burn and wound infection وبالتالي بدنا
+
+355
+00:24:24,530 --> 00:24:28,870
+نكون حذرين جدا وكل الإجراءات في قسم الحروب بتكون
+
+356
+00:24:28,870 --> 00:24:33,030
+منصبة على مدعى على مدعى وصول pseudomonas
+
+357
+00:24:33,030 --> 00:24:36,890
+aeruginosa إلى المرضى لكن شوفوا pseudomonas
+
+358
+00:24:36,890 --> 00:24:41,310
+aeruginosa كيف وصلت إلى المرضى بتعرفوا المواد
+
+359
+00:24:41,310 --> 00:24:45,050
+المطاهرة اللي لجوا فيها pseudomonas aeruginosa
+
+360
+00:24:46,080 --> 00:24:50,060
+resistant البكتيريا resistant البكتيريا resistant
+
+361
+00:24:50,060 --> 00:24:59,080
+البكتيريا
+
+362
+00:24:59,080 --> 00:25:05,100
+resistant البكتيريا
+
+363
+00:25:05,100 --> 00:25:10,280
+resistant البكتيريا
+
+364
+00:25:10,280 --> 00:25:12,620
+resistant البكتيريا resistant البكتيريا resistant
+
+365
+00:25:12,620 --> 00:25:12,640
+resistant البكتيريا resistant البكتيريا resistant
+
+366
+00:25:13,230 --> 00:25:18,890
+حمام ساخن عشان يحمموا المرضى عشان يشيلوا الجلد
+
+367
+00:25:18,890 --> 00:25:23,670
+الميت لإن الجلد الميت هذا .. الجلد الميت يعتبر
+
+368
+00:25:23,670 --> 00:25:27,410
+مادة غذائية للبكتيريا و إزالته أمر ضروري جدا
+
+369
+00:25:27,410 --> 00:25:31,810
+للعلاج و لمنع الاتهاد لما بحنا قاعد و أخدنا عينات
+
+370
+00:25:31,810 --> 00:25:35,690
+.. أخدنا من هذا ال hot tub اللي هو ال .. البانيو
+
+371
+00:25:35,690 --> 00:25:39,350
+تمام؟ و عزلنا تدورنا سريجيا مثلا .. مثلا تحكي أنه
+
+372
+00:25:39,350 --> 00:25:43,390
+مريض بيعطي كمالة ميملمريض اخر اذا كان ماكانش فيه
+
+373
+00:25:43,390 --> 00:25:46,970
+تنظيف و disinfection بشكل جيد لجينا صدموناس على
+
+374
+00:25:46,970 --> 00:25:51,490
+الصابون اللى بيستخدموه القطط بقى يبقى طيب لما يغسل
+
+375
+00:25:51,490 --> 00:25:56,630
+ايه بدل ما ينظف بياخد صدموناس رجل نفسه هذا كمان
+
+376
+00:25:56,630 --> 00:25:59,670
+مرة هذا الكلام لو كان في البيت مافيش مشكلة بس لما
+
+377
+00:25:59,670 --> 00:26:03,390
+يكون في مستشفى انتوا تذكروا انا بتكلم عن مين مريض
+
+378
+00:26:03,390 --> 00:26:09,210
+حروق يعني مالوش جلديبقى opportunistic infection
+
+379
+00:26:09,210 --> 00:26:13,190
+سهلة جدا .. سهلة جدا بينما Sodomones بالنسبة لإن
+
+380
+00:26:13,190 --> 00:26:16,090
+احنا ك healthy individuals غالبا مش هتعملنا أي
+
+381
+00:26:16,090 --> 00:26:21,210
+إشكالية إلا إذا اخترقت الجلد يعني عبر جرح أو عبر
+
+382
+00:26:21,210 --> 00:26:28,310
+.. طيب إذا لم تتم معالجتها ال barrel wound
+
+383
+00:26:28,310 --> 00:26:31,760
+infection غالبا بتقدي إلىبكتيريميا قد تصل إلى
+
+384
+00:26:31,760 --> 00:26:35,180
+نيومونيا و قد تصل إلى meningitis برضه من ال .. من
+
+385
+00:26:35,180 --> 00:26:39,420
+الأمراض المشهورة السيئة urinary tract infection في
+
+386
+00:26:39,420 --> 00:26:42,620
+البراج السباحة فيها اسمها swimmers ear بيخلص
+
+387
+00:26:42,620 --> 00:26:44,960
+السباحة بعد ساعتين تلاتة بيصير الدينه حمره
+
+388
+00:26:44,960 --> 00:26:49,080
+external ال external ear بيسموه swimmers ear من
+
+389
+00:26:49,080 --> 00:26:51,780
+اسم ال .. اللي هي أذن السباح يعني اللي بيسبحوا في
+
+390
+00:26:51,780 --> 00:26:56,350
+البراج السباحةالأذن الخارجية الـ otitis media
+
+391
+00:26:56,350 --> 00:27:00,010
+إلتهاب الأذن الداخلية الأذن الداخلية several skin
+
+392
+00:27:00,010 --> 00:27:04,630
+infections كمان برضه ممكن تعمل إلتهابات في الجلد
+
+393
+00:27:04,630 --> 00:27:06,170
+بأشكال متنوعة
+
+394
+00:27:09,430 --> 00:27:13,770
+الامراض لما احنا قولنا pseudomonas and non
+
+395
+00:27:13,770 --> 00:27:17,090
+fermentants يبقى pseudomonas هي الأشهر في المجموعة
+
+396
+00:27:17,090 --> 00:27:20,630
+خدوا بالك كل الميكروبات اللي ذكرناها في المجموعة
+
+397
+00:27:20,630 --> 00:27:24,810
+تقريبا بتسبب نفس الأمراض لكن بدرجة من أقل انتشارا
+
+398
+00:27:24,810 --> 00:27:27,510
+من pseudomonas erogenous إلى ان pseudomonas
+
+399
+00:27:27,510 --> 00:27:29,550
+erogenous في الوقت الحالي هي المسيطرة هي قائدة
+
+400
+00:27:29,550 --> 00:27:33,090
+المجموعة اللي احنا اتكلمنا عنها الدكتور انت حقيط
+
+401
+00:27:33,090 --> 00:27:35,630
+ان عزلها الصبونات في المجموعات
+
+402
+00:27:38,610 --> 00:27:42,190
+لأ هو الحل انه اول حاجة الصابون ال manufacturing
+
+403
+00:27:42,190 --> 00:27:46,170
+تبقى عقصية و لما احنا .. لما وجدنا بالفعل في
+
+404
+00:27:46,170 --> 00:27:50,070
+الصابون و كان من الفضل اللي بيرجع لطلبات من قسم
+
+405
+00:27:50,070 --> 00:27:55,130
+العلم التطبيقى المخبرية قاموا دراسة لفصفة الماضي و
+
+406
+00:27:55,130 --> 00:27:59,310
+النتائج الدراسة تم عرضها على المسؤولين في وزارة
+
+407
+00:27:59,310 --> 00:28:02,970
+الصحةوعلى مختبر الصحة العامة وعلى اللجان ال
+
+408
+00:28:02,970 --> 00:28:07,910
+infection control و أصبح هناك إجراء روتيني بتم أن
+
+409
+00:28:07,910 --> 00:28:13,110
+أي صوابين تدخل المستشفى يبقى يتم فحصها ويعدي
+
+410
+00:28:13,110 --> 00:28:15,450
+البكتيرى اللي فيها و التعرف على بعض القصاص اللي
+
+411
+00:28:15,450 --> 00:28:18,810
+فيها قبل إدخال الخدمة فكانت الدراسة الصحية لها
+
+412
+00:28:18,810 --> 00:28:23,440
+قيمة جيدة ونتج عنها actions يعنيالان الموضوع ال ..
+
+413
+00:28:23,440 --> 00:28:27,280
+ال .. ال .. ال infection control في المستشفيات،
+
+414
+00:28:27,280 --> 00:28:29,960
+هنا الصيحة عندنا لجان اسمها infection control
+
+415
+00:28:29,960 --> 00:28:33,320
+committees لكن مدى فعليتها أنا بحط عليها
+
+416
+00:28:33,320 --> 00:28:37,520
+بالاستفادة، هي إليها صيحة يمكن من ال ألف وتسعمية و
+
+417
+00:28:37,520 --> 00:28:41,300
+أربعة و تسعين و خمسة و تسعين من القرن الماضي، لكن
+
+418
+00:28:41,300 --> 00:28:45,850
+بتنشط سنةو بتقعد سنتين، تلاتة، مهمة لو أربعة و
+
+419
+00:28:45,850 --> 00:28:49,130
+بعدين بيجي واحد تاني بنشطها سنة و بتقعد .. مافيش
+
+420
+00:28:49,130 --> 00:28:52,430
+consistency فيه لذلك مالاجيش لا إجراءات مكتوبة
+
+421
+00:28:52,430 --> 00:28:55,610
+الطبيب اللي بيجي جديد مثلا أو الممرض اللي بيشتغل
+
+422
+00:28:55,610 --> 00:28:58,110
+جديد بشوف الناس بيشتغل بطريقة معينة تدخل في ال
+
+423
+00:28:58,110 --> 00:29:02,990
+system .. فغالبا مافيش عندنا system جيد الصيدل
+
+424
+00:29:02,990 --> 00:29:06,520
+infection control هذا بتطلب بالفعل أنهيكون فيه
+
+425
+00:29:06,520 --> 00:29:10,580
+قرار جريء وفيه action جريء بإنه يجب أن يكون جزء من
+
+426
+00:29:10,580 --> 00:29:13,860
+هيكرية المستشفى ان يكون فيه infection control
+
+427
+00:29:13,860 --> 00:29:17,660
+committee فعالة و تراقب و تحاسب على لو صار في out
+
+428
+00:29:17,660 --> 00:29:21,020
+break لقدر الله في المستشفى ان تحاسب على تقصيرها
+
+429
+00:29:21,020 --> 00:29:25,000
+في هذا المشكلة ال microbe التاني في السلسلة
+
+430
+00:29:25,000 --> 00:29:28,540
+الواقعية الاسينتوفاكتر بوماني هذا بصلاه ولا كوكب؟
+
+431
+00:29:35,910 --> 00:29:39,530
+هذه بتضحك عليكوا، هذه بتقولكوا أنا بس ال like لكن
+
+432
+00:29:39,530 --> 00:29:43,810
+ممكن تاخد شكل ال cooksai مين أخد cooksai gram
+
+433
+00:29:43,810 --> 00:29:49,410
+negative cooksai درسناها gram negative cooksai
+
+434
+00:29:52,980 --> 00:29:58,300
+النيسيريا .. النيسيريا هي gram-negative-diplokai،
+
+435
+00:29:58,300 --> 00:30:02,960
+الأسينيتوفاكتر mimics نيسيريا، كلمة mimics يعني
+
+436
+00:30:02,960 --> 00:30:08,000
+تحاكي، وكانوا زمان يسموها ميميا، ميميا من mimic،
+
+437
+00:30:08,000 --> 00:30:10,900
+it mimics the appearance of نيسيريا جنون
+
+438
+00:30:13,340 --> 00:30:16,700
+طيب اللي .. اللي .. اللي بتحبوش تحفظوه و بتحبوا
+
+439
+00:30:16,700 --> 00:30:21,200
+تفهموه كيف بدي اميز النيسيريا عن الأسينيتوباكتر
+
+440
+00:30:21,200 --> 00:30:24,500
+التنتين تحت الميكروسكوب وظهرولي انهم إجرام
+
+441
+00:30:24,500 --> 00:30:29,840
+نيجادردبلوكوركايف؟ كيف بدي اميزهم عن بعض؟ حكينا عن
+
+442
+00:30:29,840 --> 00:30:33,320
+الفيلم انت .. عشان انه الأسين .. الأسينيتوباكتر ..
+
+443
+00:30:33,320 --> 00:30:36,260
+ايه دخل الفيلم؟ ايه دخل؟ ال .. ال .. ال .. ال ..
+
+444
+00:30:36,260 --> 00:30:39,900
+النيسيريا .. النيسيريا أيضا aerobic النيسيريا
+
+445
+00:30:39,900 --> 00:30:42,240
+aerobic يعني بتاعة من ال oxidative بتاعة من ال
+
+446
+00:30:54,170 --> 00:31:00,510
+عن صفة اذكركوا ان الاسينيتوباكتر اكسيدايز نيجاتيف
+
+447
+00:31:00,510 --> 00:31:06,050
+بينما ان يسيريا احد اهم صفاتها و لو رجعتوا
+
+448
+00:31:06,050 --> 00:31:09,110
+للمحاضرة تبعتي هتلاقوا ان انا ركزت على كلمة
+
+449
+00:31:09,110 --> 00:31:12,530
+اكسيدايز positive على ان صفتها اكسيدايز positive
+
+450
+00:31:12,530 --> 00:31:15,510
+فإذا في يوم من الأيام انا عزلت بكتيريا
+
+451
+00:31:19,640 --> 00:31:23,520
+هو الـ Oxidase إذا Oxidase positive فهي نيسيريا
+
+452
+00:31:23,520 --> 00:31:27,080
+إذا Oxidase negative فهي أسينيتوبكتر يعني دي كتير
+
+453
+00:31:27,080 --> 00:31:32,640
+نقطة مهمة كمان مرة هي gram negative بس ال life لكن
+
+454
+00:31:32,640 --> 00:31:36,080
+it can mimic the appearance of gram negative
+
+455
+00:31:36,080 --> 00:31:42,460
+دبلوكوكتر طيب أسينيتوبكتر بوماني في عنا clinical
+
+456
+00:31:42,460 --> 00:31:45,440
+syndrome opportunistic infection أنا قلتلكوا قبل
+
+457
+00:31:45,440 --> 00:31:49,790
+شويه لسه مقدم قلتلكوا كل ال .. ال .. ال ..زميلات
+
+458
+00:31:49,790 --> 00:31:52,810
+.. زميلات Sodomonas كلهم بيسببوا تقريبا نفس
+
+459
+00:31:52,810 --> 00:31:57,690
+الأمراض لكن بدرجة أقل لكن هذا ال microbe لما احنا
+
+460
+00:31:57,690 --> 00:32:04,030
+درسناه طلاب زي حضراتكم بالفعل مر علينا كلمة واحدة
+
+461
+00:32:04,030 --> 00:32:07,690
+Acinetobacter تشبه Sodomonas لكن اكسيدايز نيجاتيب
+
+462
+00:32:07,690 --> 00:32:10,450
+وهي .. هي .. هي اللي درسناها عن ال .. ال .. ال
+
+463
+00:32:10,450 --> 00:32:13,760
+Acinetobacterاليوم الأسنة tobacco تحتلت عناوين
+
+464
+00:32:13,760 --> 00:32:18,460
+الصحف والمجلات والأخبار وصارت شهرتها زي شهر ال
+
+465
+00:32:18,460 --> 00:32:21,060
+pseudomonas و أكتر من ال pseudomonas طبعا عارفين
+
+466
+00:32:21,060 --> 00:32:26,560
+إيش اللي بيخلل بكتير تان شهر؟ إما الأمراض أو
+
+467
+00:32:26,560 --> 00:32:29,900
+صعوبتها يعني خدوا بالكوا بعض الأحيان واحد بيعمل
+
+468
+00:32:29,900 --> 00:32:34,960
+عمل واحد تمام؟ بيجلب الدنيا كلها وعمل واحد وفي ناس
+
+469
+00:32:34,960 --> 00:32:39,040
+بتعمل مليون عمل تجلب الدنيا فهذه تعمل عمل اللي هو
+
+470
+00:32:39,040 --> 00:32:43,850
+ال diseaseصار أصبح .. هدى بيسموها super bug ..
+
+471
+00:32:43,850 --> 00:32:46,790
+لبسله super bug .. سنوموله super bug .. ال .. ال
+
+472
+00:32:46,790 --> 00:32:47,030
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+473
+00:32:47,030 --> 00:32:47,530
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+474
+00:32:47,530 --> 00:32:49,030
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+475
+00:32:49,030 --> 00:32:49,590
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+476
+00:32:49,590 --> 00:32:50,090
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+477
+00:32:50,090 --> 00:32:50,110
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+478
+00:32:50,110 --> 00:32:52,750
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+479
+00:32:52,750 --> 00:32:54,070
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+480
+00:32:54,070 --> 00:32:56,310
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+481
+00:32:56,310 --> 00:33:04,210
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+482
+00:33:04,210 --> 00:33:08,550
+ال .. ال ..
+
+483
+00:33:11,230 --> 00:33:14,910
+من أول جرح أو حرق، حرق، بعدين من الدم، نفس
+
+484
+00:33:14,910 --> 00:33:18,450
+البكتيريا أسينيدوباكتر و resistant للكل و بعد فترة
+
+485
+00:33:18,450 --> 00:33:22,590
+واجيزة عضلت من ال CSF نفس المريض و نفس الشيء و بعد
+
+486
+00:33:22,590 --> 00:33:25,930
+فترة لم يقعد هناك مريض ناخد منه عينة، توفي المرض
+
+487
+00:33:27,260 --> 00:33:31,620
+لأنه أصيب بميكروب بسيط زي الacinetobacter اللي هو
+
+488
+00:33:31,620 --> 00:33:35,120
+الان super bug اللي هو multiple drug resistant و
+
+489
+00:33:35,120 --> 00:33:39,680
+غير قادر على أنه نتعامل معاه وهذا كله من ال abuse
+
+490
+00:33:39,680 --> 00:33:47,110
+لسوق الاستخدام و ال misuse لل antibioticsو توظيف
+
+491
+00:33:47,110 --> 00:33:49,730
+ال antibiotics يعني انا بقولكوا يعني معظم الناس
+
+492
+00:33:49,730 --> 00:33:54,690
+بيستخدم ال antibiotic بدون وصفة طبية و كلمة بدون
+
+493
+00:33:54,690 --> 00:33:57,390
+وصفة طبية انه يختار ال antibiotic و طبعا لو راح
+
+494
+00:33:57,390 --> 00:33:59,590
+الصيدليب يقدر يقطر الصيدليب برضه ال antibiotic على
+
+495
+00:33:59,590 --> 00:34:03,230
+.. على .. على يعني على هواه .. على هواه يمكن يكون
+
+496
+00:34:03,230 --> 00:34:07,430
+للتسويق فقط لغير مش ضرور يكون يعني انه والله فعال
+
+497
+00:34:07,880 --> 00:34:10,740
+ويمكن المرض مابحتاجش أساساً antibiotic زي ال viral
+
+498
+00:34:10,740 --> 00:34:13,380
+infections اللي معظمنا بيوصاب فيها ويقولوا انه
+
+499
+00:34:13,380 --> 00:34:16,540
+ماخد antibiotic مش هيرتاح نفسيه يعني بلاسفش هفتر
+
+500
+00:34:16,540 --> 00:34:22,140
+فللأسف هذا فيانة misuse واللي بيشجع عليه عدم وجود
+
+501
+00:34:22,140 --> 00:34:25,900
+نظام يمنع صرف الأدوية اللي زي ال antibiotic بدون
+
+502
+00:34:25,900 --> 00:34:30,570
+وصفة طبيةواللي بيزيد المشكلة أكتر عدم ولود ثقافة
+
+503
+00:34:30,570 --> 00:34:34,150
+بين الأطباء اللي هم الأصل الحمات في هذا الموضوع
+
+504
+00:34:34,150 --> 00:34:38,870
+عدم ولود الثقافة هذه أساساً فبيستمع .. بدل ما
+
+505
+00:34:38,870 --> 00:34:41,610
+يستمع لل science في ال .. في ال .. في ال
+
+506
+00:34:41,610 --> 00:34:45,030
+antibiotics بيستمع لمندبي المبيعات في الشركات
+
+507
+00:34:45,030 --> 00:34:48,090
+الأدوية اللي بيجيبوا يقولوا ال amoxicillin is
+
+508
+00:34:48,090 --> 00:34:50,850
+effective against كده و كده و كده .. هذا كلام في
+
+509
+00:34:50,850 --> 00:34:54,860
+الستنات في القرن الماضيالكلام هو صحيح، بس اليوم ال
+
+510
+00:34:54,860 --> 00:34:58,620
+amoxicillin مش effective لل .. للسفن الكوكاس اوريا
+
+511
+00:35:00,110 --> 00:35:02,910
+فانت لما انت توصف ال .. ال .. ال .. اموكسيلين
+
+512
+00:35:02,910 --> 00:35:06,610
+لمريد عنده resistant .. ال .. ال .. ال .. stuff
+
+513
+00:35:06,610 --> 00:35:09,350
+اللي بوكاس اورت resistant انت بتساهم في قتل ال
+
+514
+00:35:09,350 --> 00:35:13,110
+sensitive و بتنمي مين ال resistance فللأسف يعني
+
+515
+00:35:13,110 --> 00:35:17,030
+هذه الظاهرة موجودة بكثرة في قطاع غزة وظاهرة ملهوظة
+
+516
+00:35:17,030 --> 00:35:19,830
+يعني نرجع ان ان شاء الله حد ينتبه من المسؤولين على
+
+517
+00:35:19,830 --> 00:35:23,640
+حوالي قدر السطوع الصحيح يعني انا خطبتووزير الصحة
+
+518
+00:35:23,640 --> 00:35:27,380
+كتاب كامل ابعتله خطط عمل كيف يستغلوا البيانات
+
+519
+00:35:27,380 --> 00:35:31,520
+الموجودة عندهم بحيث انه على الأقل يصدر تقرير كل 6
+
+520
+00:35:31,520 --> 00:35:35,560
+أشهر يعطي صورة واضحة عن مدى ال resistance في ال
+
+521
+00:35:35,560 --> 00:35:39,200
+antibiotics، بيطلع تقارير لكن متقطع، مش دايما احنا
+
+522
+00:35:39,200 --> 00:35:45,120
+كنا متقططينمافيش أي إجراءات اتقدت من ذاك الوقت إلى
+
+523
+00:35:45,120 --> 00:35:49,580
+اليوم لتحسين من صرف ال antibiotics وترشيد
+
+524
+00:35:49,580 --> 00:35:53,260
+استهلاكها وتنظيم عملها للأسف مافيش قوانين طلعت
+
+525
+00:35:53,260 --> 00:35:56,420
+بهذا المشكلة
+
+526
+00:36:01,430 --> 00:36:05,490
+Bones and septicemia الإبديمولوجي كلمة niches اللي
+
+527
+00:36:05,490 --> 00:36:10,610
+هي يعني الأماكن الصغيرة نوصف فيها مثلا بيقول الفم
+
+528
+00:36:10,610 --> 00:36:16,170
+احنا الفم بيئة واحدة ولا بيئات بيئات حاجة اتفقنا
+
+529
+00:36:16,170 --> 00:36:21,850
+كل بيئة في الفم بيسميها niche فوين الخروم؟ خروم
+
+530
+00:36:21,850 --> 00:36:26,670
+بيئي بالأحرى natural environments في الطبيعة
+
+531
+00:36:26,670 --> 00:36:30,530
+الموجودة في الميه في النباتات moist surfaces in
+
+532
+00:36:30,530 --> 00:36:35,200
+hospitalsزي مثلا Respiratory Therapy و الناس
+
+533
+00:36:35,200 --> 00:36:40,960
+بتعرفوا اللي محطها مثلا على الهادره في بعض الأجهزة
+
+534
+00:36:40,960 --> 00:36:43,320
+اللي .. اللي .. اللي بيحطوها في ال .. ما بتشوفوهاش
+
+535
+00:36:43,320 --> 00:36:45,840
+انتوا هي اللي بيحطوها في ال intensive care unit
+
+536
+00:36:45,840 --> 00:36:50,000
+اللي بتبقى .. اللي بتوفر pressure معين في ال lungs
+
+537
+00:36:50,000 --> 00:36:55,190
+فالمريض ماتنفسش من .. من نفسه هوطبعا يتولد لغوى
+
+538
+00:36:55,190 --> 00:36:59,490
+اصبعا عندها دي برضه كمان بيكون موجود فيها نوع من
+
+539
+00:36:59,490 --> 00:37:03,110
+الترطيب عشان ماتجفش ال lungs فبرضه البكتيريا
+
+540
+00:37:03,110 --> 00:37:06,930
+الاسينتروبكتر ممكن تأنه فيه لذلك بيلاجي في
+
+541
+00:37:06,930 --> 00:37:10,330
+المستشفيات اكتر استخدام لل antibiotic لانه بنعمل
+
+542
+00:37:10,330 --> 00:37:15,470
+prophlaxis بنمنع بكتيريا تقتل كل مزارة إجراءاتنا
+
+543
+00:37:15,470 --> 00:37:19,410
+اللي بنسميها invasive proceduresكل ميلاد إمكانية
+
+544
+00:37:19,410 --> 00:37:24,790
+إنه ننقل ميكروبات للمرضى أعطيكم مثال صغير baby طفل
+
+545
+00:37:24,790 --> 00:37:29,110
+في الحضانة نتحصله كل ست ساعات arterial blood gas
+
+546
+00:37:29,110 --> 00:37:31,510
+إيش يعني arterial blood gas؟ يعني ناخدله arterial
+
+547
+00:37:31,510 --> 00:37:35,070
+blood عشان ناخدله arterial blood بنحط إيدينا على
+
+548
+00:37:35,070 --> 00:37:40,090
+مكان ونعمل palpitation نحس وننبض لمام تبع الفمورة
+
+549
+00:37:40,090 --> 00:37:45,390
+الارترية و بعدين نمسح كهول و بعدين ندخل نيدل طبعا
+
+550
+00:37:45,390 --> 00:37:49,050
+لو مرض بالي بحط إصبعهتمام؟ و بمسحها فى كهول بنسى و
+
+551
+00:37:49,050 --> 00:37:52,690
+انا بقعد برجع كمان مرة أو بحطه القبرة كل ما اتعرض
+
+552
+00:37:52,690 --> 00:37:55,990
+الطفل مرارا و تكرارا لها ده العملية كل ما زاد فرصة
+
+553
+00:37:55,990 --> 00:37:59,710
+انه ايه؟ انه يصاب زى ما احنا كل ما روحنا على ..
+
+554
+00:37:59,710 --> 00:38:03,830
+على الجامعة بالمواصلة فرصة انه يصاب بحادث سيارة
+
+555
+00:38:03,830 --> 00:38:06,550
+بتبتعه لأ ليه بروح مرة ده؟ ليه بروح مرة تانية؟
+
+556
+00:38:06,550 --> 00:38:11,500
+مظبط ولا لأ؟ فنفس الجثة كل واحد اتعرض لأعجبتكم
+
+557
+00:38:11,500 --> 00:38:16,440
+الفكرة؟ كل ما كبر واحد في السن فرصة ان ينصف بأمراض
+
+558
+00:38:16,440 --> 00:38:22,820
+معينة او بحوادث سيارات برضه بتكون اكبر طيب dry
+
+559
+00:38:22,820 --> 00:38:27,080
+surfaces زي human skin rare for a gram negative بس
+
+560
+00:38:27,080 --> 00:38:30,000
+الله يعني هذه الحادثة غيرها غالبا بنلاقيش على
+
+561
+00:38:30,000 --> 00:38:33,240
+الجلد تبقى على الجهة gram negative بس الله لكن
+
+562
+00:38:33,240 --> 00:38:36,350
+الأسنان دوبة أكتر بتكون موجودةoccasionally ممكن
+
+563
+00:38:36,350 --> 00:38:39,430
+تكون normal flora in the oropharynx يعني ممكن تكون
+
+564
+00:38:39,430 --> 00:38:42,990
+مولودة في الفموي الاند أكتر treatment prevention
+
+565
+00:38:42,990 --> 00:38:45,670
+and control antibiotic bacteria resistant is
+
+566
+00:38:45,670 --> 00:38:48,150
+common empirical treatment كلمة empirical
+
+567
+00:38:48,150 --> 00:38:52,230
+treatment يعني العلاج النظري وهذا النقطة اللي لازم
+
+568
+00:38:52,230 --> 00:38:56,570
+نفهمها ايش يعني علاج نظري؟ لو انا عرفت ان هذا
+
+569
+00:38:56,570 --> 00:39:03,080
+المريض عنده infection بقى سنة و بقتمعلوماتي ان
+
+570
+00:39:03,080 --> 00:39:06,060
+الاسنين التباكتر تستجيب ل antibiotic x و y و z
+
+571
+00:39:06,060 --> 00:39:12,480
+فبوصف لوحد من ال antibiotics دولة تمام بما يتلقى
+
+572
+00:39:12,480 --> 00:39:16,560
+مع المريض إذا كان أنثى حامل مش حامل طفل كبير مظبوط
+
+573
+00:39:16,560 --> 00:39:19,930
+فيه اعتبارات معينة صار في ال antibioticهذا بيسموه
+
+574
+00:39:19,930 --> 00:39:23,370
+empirical لكن لما أنا بعمل sensitivity testing
+
+575
+00:39:23,370 --> 00:39:26,210
+للأسنين تبقى أكتر و بقول له هذه sensitive لكذا و
+
+576
+00:39:26,210 --> 00:39:29,910
+resistant لكذا، بيبطل empirical، بيصير مبني على
+
+577
+00:39:29,910 --> 00:39:33,890
+اللي هو sensitivity testing، إذن ال empirical مبني
+
+578
+00:39:33,890 --> 00:39:36,890
+على معلومات نظرية، طب هذه المعلومات النظرية مش هي
+
+579
+00:39:36,890 --> 00:39:42,880
+متغيرة؟ بمعنى إنه في فلسطين غير عن أمريكافي العراق
+
+580
+00:39:42,880 --> 00:39:48,480
+غير عن سوريا لإن كل واحد له أسلوب في صرف الأدوية
+
+581
+00:39:48,480 --> 00:39:51,840
+ويله قوانينه تمام؟ ويله أصناع Antibiotics ممكن
+
+582
+00:39:51,840 --> 00:39:56,540
+تختلف عن الأخرى في البلدان التانية فواضح من ال ..
+
+583
+00:39:56,540 --> 00:40:00,220
+ال .. ال .. الكلام إن ال empirical therapy يجب أن
+
+584
+00:40:00,220 --> 00:40:07,000
+يكون مبني على بيانات .. بيانات محليةlocal جات عشان
+
+585
+00:40:07,000 --> 00:40:12,140
+انا بقول الدفاقة لكوارثات الصحة اللي فيها نتائج ال
+
+586
+00:40:12,140 --> 00:40:17,740
+sensitivity لو اتجمعت و اتصنفت و اتحللت و انبعتت
+
+587
+00:40:17,740 --> 00:40:22,660
+لطباء كل ست شهور بصير الدكتورة عارف جاتيش احتمالية
+
+588
+00:40:22,660 --> 00:40:26,240
+ان لو اعطى amoxicillin ان المرض يشفع من أسلوبه
+
+589
+00:40:26,240 --> 00:40:30,000
+بكتر، مظبوط ولا لأ؟ بس لما يكون مبني بياناته على
+
+590
+00:40:30,000 --> 00:40:34,890
+أوروبا أو على أمريكا او على textbook له عشرين سنةو
+
+591
+00:40:34,890 --> 00:40:40,470
+للأسف .. للأسف هذا ما يحدث في معظم الأحيان ليست
+
+592
+00:40:40,470 --> 00:40:44,830
+مبنية كمان مرة عالمين على local لأ و هذه نقطة
+
+593
+00:40:44,830 --> 00:40:49,190
+خطيرة جدا أنا بحد أنه أروح إليها باستمرار specific
+
+594
+00:40:49,190 --> 00:40:52,930
+therapy according to antibiotic susceptibility is
+
+595
+00:40:52,930 --> 00:40:56,790
+required أخر جملة في المحاضرة و أعتقد أن اليوم
+
+596
+00:40:56,790 --> 00:41:00,170
+بكفينا ان شاء الله نكمل المرة الجاية بالمراجز اللي
+
+597
+00:41:00,170 --> 00:41:00,950
+كتيرانس
+
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/m_if8j49Njk_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/m_if8j49Njk_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..1b511696fd0957f0a184c84d484f2e689b99570a
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/m_if8j49Njk_raw.json
@@ -0,0 +1 @@
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هيطلع النتائج النهائية المهمة؟", "tokens": [20498, 1863, 11296, 2423, 32277, 9957, 36081, 37746, 995, 4117, 2655, 2288, 13672, 1829, 31439, 2107, 12, 75, 578, 541, 38300, 260, 4724, 3794, 27188, 2288, 4587, 49374, 2423, 3242, 996, 14125, 279, 13672, 1829, 29910, 37983, 2655, 16095, 4032, 49374, 2423, 25505, 532, 24488, 296, 2423, 15508, 5076, 6497, 623, 289, 1975, 1829, 8592, 39896, 9566, 1211, 3615, 2423, 15508, 5076, 6497, 623, 289, 6055, 3555, 3615, 2423, 25505, 532, 24488, 296, 22807, 15508, 5076, 6497, 623, 289, 6055, 3555, 3615, 2423, 25505, 532, 24488, 296, 1975, 1829, 8592, 39896, 9566, 1211, 3615, 22807, 1975, 1829, 8592, 39896, 9566, 1211, 3615, 28239, 2655, 16373, 7435, 28239, 3224, 16373, 10632, 9673, 16095, 3660, 22807], "avg_logprob": -0.2523097950479259, "compression_ratio": 1.995, "no_speech_prob": 0.0, "words": [{"start": 1300.5, "end": 1300.98, "word": "ومنها", "probability": 0.6947428385416666}, {"start": 1300.98, "end": 1301.9, "word": " الاسينيتوباكتر", 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بيستمع لمندبي المبيعات في الشركات الأدوية اللي بيجيبوا يقولوا ال amoxicillin is effective against كده و كده و كده .. هذا كلام في الستنات في القرن الماضي", "tokens": [2407, 6027, 20292, 4724, 1829, 11622, 25708, 9673, 8592, 28820, 3660, 5551, 4117, 2655, 2288, 6225, 40448, 35525, 23328, 38637, 4587, 31845, 3660, 49374, 16247, 9566, 3555, 16606, 13672, 1829, 8032, 2304, 16247, 36520, 21542, 2304, 9307, 8978, 23758, 9673, 2407, 11242, 45367, 6225, 40448, 35525, 23328, 6024, 104, 4587, 31845, 3660, 29538, 5551, 3794, 32277, 995, 14111, 6156, 21292, 14851, 2304, 3615, 4386, 47525, 1211, 19446, 7251, 14851, 2304, 3615, 24976, 3497, 8978, 2423, 4386, 8978, 2423, 4386, 8978, 2423, 26922, 4724, 1829, 14851, 2304, 3615, 5296, 27842, 3215, 21292, 9673, 21292, 3615, 9307, 8978, 25124, 31747, 9307, 16247, 3215, 2407, 10632, 13672, 1829, 4724, 1829, 7435, 1829, 3555, 14407, 7251, 39648, 14407, 2423, 669, 5230, 299, 373, 259, 307, 4942, 1970, 9122, 3215, 3224, 4032, 9122, 3215, 3224, 4032, 9122, 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diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/nuUmNBHfSpw_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/nuUmNBHfSpw_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..874db562c2c721dfaaff026675f8ad931869c56a
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/nuUmNBHfSpw_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 4324, "start": 20.96, "end": 43.24, "text": "بسم الله الرحمن الرحيم نذكركم بموضوع المحاضرة اللي هو الجزء التاني من ال sterilization and disinfection ونحن مرمور سريع على اللوحات اللي درسناها المحاضرة السابقة ونذكركم أنه احنا ميزنا بين ال sterilization كمصطلح مطلق وبين disinfection اللي هو ثيقة ولكن ليس جميلة", "tokens": [3555, 38251, 21984, 34892, 5016, 27842, 34892, 5016, 32640, 8717, 8848, 37983, 24793, 4724, 2304, 2407, 11242, 45367, 9673, 5016, 46958, 25720, 13672, 1829, 31439, 25724, 11622, 38207, 16712, 7649, 1829, 9154, 2423, 41477, 2144, 293, 33334, 313, 4032, 1863, 5016, 1863, 3714, 2288, 2304, 13063, 8608, 16572, 3615, 15844, 13672, 2407, 5016, 9307, 13672, 1829, 11778, 2288, 3794, 8315, 11296, 9673, 5016, 46958, 25720, 21136, 16758, 28671, 4032, 1863, 8848, 37983, 24793, 14739, 3224, 1975, 5016, 8315, 3714, 1829, 11622, 8315, 49374, 2423, 41477, 2144, 9122, 2304, 9381, 9566, 1211, 5016, 3714, 9566, 1211, 4587, 46599, 9957, 33334, 313, 13672, 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heat سرعة وصول الحرارة الى ال center من الجانب اللي فيه ال wet heat اعلى من سرعة وصول ال dry heat الى .. يعني قدرة ال penetration بتكون يعني قدرة اعلى كمان اخرى", "tokens": [40228, 22653, 4724, 9778, 9640, 9673, 16606, 43242, 3224, 12174, 3215, 25720, 2423, 35187, 37495, 22653, 1975, 8315, 1975, 5172, 43042, 16472, 3224, 18871, 16254, 1975, 8315, 44713, 2407, 7649, 3660, 2423, 3056, 6055, 3555, 3615, 11296, 34105, 4724, 15040, 6055, 3794, 9778, 9957, 9154, 34105, 6630, 3738, 4032, 9154, 34105, 4016, 3738, 8608, 2288, 27884, 4032, 9381, 12610, 21542, 2288, 9640, 3660, 2423, 7578, 2423, 3056, 9154, 25724, 7649, 3555, 13672, 1829, 8978, 3224, 2423, 6630, 3738, 1975, 3615, 23942, 9154, 8608, 2288, 27884, 4032, 9381, 12610, 2423, 4016, 3738, 2423, 7578, 4386, 37495, 22653, 12174, 3215, 25720, 2423, 35187, 39894, 30544, 37495, 22653, 12174, 3215, 25720, 1975, 3615, 23942, 9122, 2304, 7649, 1975, 34740, 7578], "avg_logprob": -0.24457097015643525, "compression_ratio": 2.071111111111111, 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\ No newline at end of file
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/nuUmNBHfSpw_raw.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/nuUmNBHfSpw_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..c80debf6ea1281fd847d3c33e6e983c262d0f0f5
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/nuUmNBHfSpw_raw.srt
@@ -0,0 +1,2704 @@
+1
+00:00:20,960 --> 00:00:26,560
+بسم الله الرحمن الرحيم نذكركم بموضوع المحاضرة اللي
+
+2
+00:00:26,560 --> 00:00:29,040
+هو الجزء التاني من ال sterilization and
+
+3
+00:00:29,040 --> 00:00:32,240
+disinfection ونحن مرمور سريع على اللوحات اللي
+
+4
+00:00:32,240 --> 00:00:36,320
+درسناها المحاضرة السابقة ونذكركم أنه احنا ميزنا
+
+5
+00:00:36,320 --> 00:00:39,520
+بين ال sterilization كمصطلح مطلق وبين disinfection
+
+6
+00:00:39,520 --> 00:00:45,760
+اللي هو ثيقة ولكن ليس جميلةتعبثنا عن مصطلحين مهمين
+
+7
+00:00:45,760 --> 00:00:49,700
+جدا يقولنا disinfectant و antiseptic التمييز بينهم
+
+8
+00:00:49,700 --> 00:00:54,420
+كان بحسب الاستخدامتحدثنا كدا عن معادلة مهمة جدنا
+
+9
+00:00:54,420 --> 00:00:59,400
+تقولنا إن ال number of survivors يتناسب .. يتناسبا
+
+10
+00:00:59,400 --> 00:01:03,340
+عكسيا مع كل من الزمن و التركيز و لاجينا إن ال
+
+11
+00:01:03,340 --> 00:01:07,380
+concentration في exception و أخدنا الكحول ك .. ك
+
+12
+00:01:07,380 --> 00:01:10,000
+.. كنموضة لل exception إنه لما صعد ال
+
+13
+00:01:10,000 --> 00:01:13,440
+concentration 100% فقدت .. فقدت القدرة اللي ذكرنا
+
+14
+00:01:13,440 --> 00:01:19,790
+اللي هو تبرير لهذا النوعجسمنا أيضا المواهد
+
+15
+00:01:19,790 --> 00:01:22,410
+المستخدرة في ال sterilization أو ال disinfection
+
+16
+00:01:22,410 --> 00:01:25,710
+إلى تسماء إما chemical agent أو physical agent و
+
+17
+00:01:25,710 --> 00:01:29,090
+بعدين بحديث عن ال chemical agent لكن قبل ما تحدثنا
+
+18
+00:01:29,090 --> 00:01:31,370
+عن ال chemical agent تحدثنا عن مصطلح اسمه phenol
+
+19
+00:01:31,370 --> 00:01:36,370
+coefficient و تحدثنا بأن ال phenol coefficient هو
+
+20
+00:01:36,370 --> 00:01:38,930
+عملية مقارنة ما بين
+
+21
+00:01:47,070 --> 00:01:51,050
+ذكرنا ثلاثة آليات للقتل أو لإيقاف النشاط بالنسبة
+
+22
+00:01:51,050 --> 00:01:55,930
+لـdisinfectant الchemical cell membrane disruption
+
+23
+00:01:55,930 --> 00:02:00,470
+protein denaturation و DNA modification أو
+
+24
+00:02:00,470 --> 00:02:06,150
+denaturation whatsoever ذكرنا الكحولاء و المنظرفات
+
+25
+00:02:08,840 --> 00:02:11,780
+مركبات وعرفنا كل واحد منهم كيف الميكانيزم of
+
+26
+00:02:11,780 --> 00:02:15,780
+action وارترقنا الى مصطلح اسمه oligodynamic action
+
+27
+00:02:15,780 --> 00:02:20,700
+وهذا المصطلح جُننا او بررنا من خلاله لماذا لم تكون
+
+28
+00:02:20,700 --> 00:02:25,260
+البكتيليا لحد الآن resistance عالى لهذه المركبات
+
+29
+00:02:25,260 --> 00:02:27,980
+نظرا لان القتل ..
+
+30
+00:02:31,870 --> 00:02:35,970
+اليوم هنبدأ بالحديث عن الجلد التاني من ال chemical
+
+31
+00:02:35,970 --> 00:02:39,590
+disinfectants اللي هم بيشتغلوا على ال protein
+
+32
+00:02:39,590 --> 00:02:41,810
+synthesis و هتكلم عن الكلورين والأيودين
+
+33
+00:02:41,810 --> 00:02:45,210
+والهيديميتل، الهيدرينبروقصيد الفورمالبيهيد و
+
+34
+00:02:45,210 --> 00:02:48,830
+اليوترالبيهيد، الاتريلينوكسيد و الاسيدس و
+
+35
+00:02:48,830 --> 00:02:52,720
+الالكاستسالكلورين أعتقد بداية المقدمة المرة
+
+36
+00:02:52,720 --> 00:02:57,360
+الماضية، لكن لا بأس إنه نتحدث فيه، الكلورين يعتبر
+
+37
+00:02:57,360 --> 00:03:02,560
+powerful oxidizing agent وبيعمل killing للبكتيريا
+
+38
+00:03:02,560 --> 00:03:06,160
+عن طريق ال cross linking لل essential self
+
+39
+00:03:06,160 --> 00:03:07,840
+-hydrating group، تعرف ال self-hydrating group
+
+40
+00:03:07,840 --> 00:03:12,470
+اللي هي ال SHو ال S-H في أي بروتين الو ترشري أو
+
+41
+00:03:12,470 --> 00:03:17,570
+الو folding الو بيحفظ ال tertiary structure هو ال
+
+42
+00:03:17,570 --> 00:03:23,510
+S-H binding فلما نجيب S-H منها و نربطهم مع بعض
+
+43
+00:03:23,510 --> 00:03:28,290
+بهذا الشكل يبقى هنغير في شكل مين لبروتين كلمة
+
+44
+00:03:28,290 --> 00:03:32,490
+تغيير في الشكل أنا بدي أجف عندها لأنها مفهوم لازم
+
+45
+00:03:32,490 --> 00:03:38,320
+يعني نعرفه كويسمفهوم بننصيه بعبارة أو بعبارة دقيقة
+
+46
+00:03:38,320 --> 00:03:42,860
+نحفظها اسمها structure-function relationship علاقة
+
+47
+00:03:42,860 --> 00:03:49,280
+التركيب بالوظيفة التركيب بالوظيفة لان هبنعرف ان ال
+
+48
+00:03:49,280 --> 00:03:54,660
+enzyme الوه active site مظبوط ال active site هذا
+
+49
+00:03:54,660 --> 00:04:02,160
+بيشتغل مع substrate محدد ليش؟ لانه شكله مش محدد لو
+
+50
+00:04:02,160 --> 00:04:04,200
+صار تغيير في ال
+
+51
+00:04:07,880 --> 00:04:11,440
+يبجأ أي تغيير في الـ structure أدى إلى تغيير في
+
+52
+00:04:11,440 --> 00:04:14,480
+مين؟ في الوظيفة، وكلمة تغيير في الوظيفة معناها
+
+53
+00:04:14,480 --> 00:04:18,940
+ممكن فقدان الوظيفة، فقدان الوظيفة، وهذا على
+
+54
+00:04:18,940 --> 00:04:22,600
+الصراحة هيبقى دي مثال بسيط بعيدا عن الموضوع عشان
+
+55
+00:04:22,600 --> 00:04:28,220
+نوضح الفكرة، يد الإنسان ويد القلب، هدي يد و هدي
+
+56
+00:04:28,220 --> 00:04:32,620
+يد، لكن بما نعرف أنه anatomically في slight
+
+57
+00:04:32,620 --> 00:04:36,570
+modificationفي ال .. في ال .. يعني مش .. مش .. مش
+
+58
+00:04:36,570 --> 00:04:41,370
+identical hands تمام؟هذا الفرق البسيط اللى احنا
+
+59
+00:04:41,370 --> 00:04:45,130
+ممكن نشوفه شكلا تمام هو اللى أدى الى القدرة
+
+60
+00:04:45,130 --> 00:04:49,390
+الرائعة للإنسان على التحكم في أصابعه وقدرته على
+
+61
+00:04:49,390 --> 00:04:53,750
+الإمساك بالقلم والكتابة أو الطباعة أو عمل أشياء
+
+62
+00:04:53,750 --> 00:04:57,650
+اللى لن يستطيع القرد لو قعد ميت سنة نعلم فيه
+
+63
+00:04:57,650 --> 00:05:01,930
+النجوم فيها وفي نفس الوقت جعل عند القرد القدرة على
+
+64
+00:05:01,930 --> 00:05:04,730
+التسلق اللى احنا نرعى والمهارة العالية اللى احنا
+
+65
+00:05:04,730 --> 00:05:09,840
+مقدرش عليهاأي modification في ال structure قد يؤدي
+
+66
+00:05:09,840 --> 00:05:12,560
+إلى modification في من؟ ال function في الوظيفة أو
+
+67
+00:05:12,560 --> 00:05:15,840
+في اللي هي ال function هذه نقطة كتير مهمة إذا لما
+
+68
+00:05:15,840 --> 00:05:18,980
+هقول modification of proteins بواقع الأمر احنا
+
+69
+00:05:18,980 --> 00:05:22,820
+بنعمل عملية تغيير في تركيب ال protein أو طبيعة شكل
+
+70
+00:05:22,820 --> 00:05:26,360
+ال protein وبالتالي بنؤدي إلى تغيير في من؟ ال
+
+71
+00:05:26,360 --> 00:05:31,110
+functionطبعا بتسمعوا بالchlor و بتستخدموه في
+
+72
+00:05:31,110 --> 00:05:34,990
+البيوت للتنظيف غالبا لكن برضه ممكن تستخدموه في
+
+73
+00:05:34,990 --> 00:05:40,110
+التطهير في الحفامات يبقى هو الchlorox الاسم
+
+74
+00:05:40,110 --> 00:05:43,570
+المشهور أو ال bleach باللغة الإنجليزية مشهور برضه
+
+75
+00:05:43,570 --> 00:05:48,710
+اللي بيحطوه عشان يبيضوا الملابس التركيبة الأساسية
+
+76
+00:05:48,710 --> 00:05:53,230
+في مين؟ الchlor الاستخدام الرئيسي للchlor في ال
+
+77
+00:05:53,230 --> 00:05:57,110
+disinfection هو في water أو drinking water
+
+78
+00:05:57,110 --> 00:06:01,010
+disinfectionأيضا في ال swimming pools في براك
+
+79
+00:06:01,010 --> 00:06:04,690
+السبح إذا هناك استخدامات كثيرة ومتنوعة لكن فهمنا
+
+80
+00:06:04,690 --> 00:06:08,130
+ال mechanism of action تبعت إيه لفريقنا نجي
+
+81
+00:06:08,130 --> 00:06:12,940
+للأيودينالايودين خد بالكه له تقريبا نفس القدرة و
+
+82
+00:06:12,940 --> 00:06:17,720
+أفضل من اللي هو الكلورين لكن لماذا لما نستخدم
+
+83
+00:06:17,720 --> 00:06:24,420
+الايودين بيترك أثر و تعرفين أنه من مزايا الماء أنه
+
+84
+00:06:24,420 --> 00:06:29,180
+لا لون ولا رائحة فبالتالي يعني وجود أيودين في
+
+85
+00:06:29,180 --> 00:06:32,700
+الماء هيعطي لون غير مقبول حتى وإن كان هو طهر أو
+
+86
+00:06:32,700 --> 00:06:38,160
+آمن للاستخدام لكن الايودين يستخدم استخدام
+
+87
+00:06:44,170 --> 00:06:50,950
+أو قبل العملية الجراحية لقتل ال micro organism قبل
+
+88
+00:06:50,950 --> 00:06:54,930
+إحداث الجروح في العملية الجراحية
+
+89
+00:06:57,860 --> 00:07:02,440
+شكلين أساسيين للأيودين في الواقع الشكل الأول
+
+90
+00:07:02,440 --> 00:07:07,000
+بيسموه iodine tincture صبغة الأيودين وهو مكوّن من
+
+91
+00:07:07,000 --> 00:07:11,920
+2% من 2% من ال أيودين and potassium iodide إذا
+
+92
+00:07:11,920 --> 00:07:15,180
+انخلط من الأيودين and potassium iodide التركيز سبب
+
+93
+00:07:15,180 --> 00:07:20,280
+هو 2% وزي ما ذكرلكم بيستخدموه في اللي هو ال blood
+
+94
+00:07:20,280 --> 00:07:24,820
+culture collection ك skin antisepticالان الجملة
+
+95
+00:07:24,820 --> 00:07:27,400
+الأخيرة اللى مذكورة راكوا على اللوحة بنتبه إليها
+
+96
+00:07:27,400 --> 00:07:31,280
+لإنه مش هي الجملة الوحيدة اللى ممكن تنحط في هذا
+
+97
+00:07:31,280 --> 00:07:34,400
+السياق because the texture of iodine can be
+
+98
+00:07:34,400 --> 00:07:37,380
+irritating to the skin it should be removed with
+
+99
+00:07:37,380 --> 00:07:37,820
+alcohol
+
+100
+00:07:41,660 --> 00:07:45,620
+لكن مش بس لإنه بيبقى ال irritation لل skin، أيضا
+
+101
+00:07:45,620 --> 00:07:50,620
+أنا مش معنى أترك أي أثر لل أيودين، ليش؟ عندما ندخل
+
+102
+00:07:50,620 --> 00:07:55,020
+ال .. ال needle إلى ال vein اللي على الجلب كان فيه
+
+103
+00:07:55,020 --> 00:07:58,580
+أيودين، لو نفرض إنه فيه متبقيات، هندخل .. احنا
+
+104
+00:07:58,580 --> 00:08:02,780
+معانا مين؟ ال أيودين داخل ال .. ال bloodو لما ناخد
+
+105
+00:08:02,780 --> 00:08:06,040
+الدم يمكن يتوعب معانا ال answer فيه ان دي blood
+
+106
+00:08:06,040 --> 00:08:09,280
+فيه microorganism و فيه أيودين طب الأيودين هيقتل
+
+107
+00:08:09,280 --> 00:08:11,880
+البكتيريا طب انا اخدي العيل عشان اقتل البكتيريا
+
+108
+00:08:11,880 --> 00:08:16,620
+عشان اناميها عشان اناميها وبالتالي يبقى فيه غرضين
+
+109
+00:08:16,620 --> 00:08:21,140
+لإزالة الأيودين ان واحد irritant رقم اتنين to
+
+110
+00:08:21,140 --> 00:08:25,040
+avoid اللي هو false negative results
+
+111
+00:08:29,180 --> 00:08:32,240
+تطلع معانا negative لكن مش negative حقيقي ال
+
+112
+00:08:32,240 --> 00:08:37,100
+negative سببه وجود اللي هو الايودين اتفقنا على
+
+113
+00:08:37,100 --> 00:08:40,960
+الشكل الاول من الايودين، texture of iodine الشكل
+
+114
+00:08:40,960 --> 00:08:46,340
+التاني بيسموه Iodophores وال Iodophores هي عبارة
+
+115
+00:08:46,340 --> 00:08:52,060
+عن خليط، هجين، ما بين الايودين وال detergent وهي
+
+116
+00:08:52,060 --> 00:08:55,080
+مركبات معقدة، يعني مش مجرد انه خلطهم مع بعض، بقى
+
+117
+00:08:55,080 --> 00:08:59,290
+هى تفاعل كيموي بيصير بينهمالـ detergent المحاضرة
+
+118
+00:08:59,290 --> 00:09:02,590
+السابقة ذكرنا عنه ان هو mild antiseptic skin
+
+119
+00:09:02,590 --> 00:09:06,910
+antiseptic mild يعني لطيف خفيف تمام لكن قدرته على
+
+120
+00:09:06,910 --> 00:09:12,470
+القتل الصحيح قليلا بينما الأيودين بيطلقوا عليه اسم
+
+121
+00:09:12,470 --> 00:09:15,790
+germicidal لأنه عنده المقضة على قتل الفيروسات و
+
+122
+00:09:15,790 --> 00:09:18,610
+على قتل حتى ال sports أو بعض ال sports نحن نقول ال
+
+123
+00:09:18,610 --> 00:09:22,190
+sports كلها ده ده بيسموه germicidal germicidal
+
+124
+00:09:22,190 --> 00:09:27,710
+فلما نخلط واحد لطيفمع واحد قاتل لكن إله irritation
+
+125
+00:09:27,710 --> 00:09:32,370
+أعطاه المست المستين إنه صار اليودوفور جنتل على ال
+
+126
+00:09:32,370 --> 00:09:36,690
+skin و في نفس الوجد كفاته في القتل زي ما هي أو حتى
+
+127
+00:09:36,690 --> 00:09:41,950
+زادت و رسحنا كده مرة في معالجة أو تطهير الجلد قبل
+
+128
+00:09:41,950 --> 00:09:48,190
+العمليات الجراحية الايوديين بيشتغل بنفس القليل يا
+
+129
+00:09:48,190 --> 00:09:51,230
+بنات اللي بيشتغل فيها الكلورين لأنهم ينتموا بنفس
+
+130
+00:09:51,230 --> 00:09:52,770
+العائلة
+
+131
+00:09:54,270 --> 00:09:58,350
+أبعد عن Oxidant و بيعمل Inactivation للـ self
+
+132
+00:09:58,350 --> 00:10:02,190
+-hydril containing enzymes لأن ال enzymes ما هي
+
+133
+00:10:02,190 --> 00:10:06,510
+proteins صح؟ لما يكون فيها self-hydril ال self
+
+134
+00:10:06,510 --> 00:10:09,390
+-hydril بيصير ال inactivation الإنزايم بيفقد الشكل
+
+135
+00:10:09,390 --> 00:10:12,890
+تبعه وبالتالي بيفقد الوظيفة تبعه في كانت هنا
+
+136
+00:10:12,890 --> 00:10:16,110
+ميجانيزم إضافية إنه بيرتبط بشكل specific مع
+
+137
+00:10:16,110 --> 00:10:19,770
+التيروسين وهذا أيضا يؤدي إلى destruction ل ال
+
+138
+00:10:19,770 --> 00:10:19,970
+protein
+
+139
+00:10:23,340 --> 00:10:27,440
+يجي ال heavy metals ال heavy metals بتعرفون انتوا
+
+140
+00:10:27,440 --> 00:10:30,860
+منها الذئبق و بتعرفون انها النحاس و بتعرفون انها
+
+141
+00:10:30,860 --> 00:10:35,300
+الفضة نمب انسوا اللي بقولها اللوحة شوية و خدنا
+
+142
+00:10:35,300 --> 00:10:40,180
+نتكلم بالموضوع بيقوموا بيها لو
+
+143
+00:10:40,180 --> 00:10:43,780
+احنا امتلكنا أسطح زي الطرد اللي جاني عليها هذا
+
+144
+00:10:43,780 --> 00:10:45,900
+الشكل لو هي عليها بكتيريا
+
+145
+00:10:52,070 --> 00:10:58,130
+أصبح ورقة بفعل ال heavy metals خاصة ال silver ال
+
+146
+00:10:58,130 --> 00:11:02,570
+silver اللي هو الفضة الان لو جبنا .. طبعا هنا في
+
+147
+00:11:02,570 --> 00:11:05,170
+تقنية اسمها nano technology اللي هي تقنية ال nano
+
+148
+00:11:05,170 --> 00:11:10,410
+بنصنع جزيئات من ال silver nano particles ال size
+
+149
+00:11:10,410 --> 00:11:13,510
+تبعها ما بين 1 nano ل 100 nano اقل من 100 nano
+
+150
+00:11:13,510 --> 00:11:18,030
+يعني اتنين تلاتة nano بعدين بطريقة معينة بنفردهم
+
+151
+00:11:18,030 --> 00:11:24,360
+زي البوية على الأسطحوبينه بيجففوا لأن الميزة هنا
+
+152
+00:11:24,360 --> 00:11:29,160
+في ال nano particles انه very small قدرتها على ال
+
+153
+00:11:29,160 --> 00:11:32,780
+interaction بتكون عالية جدا أعلى من ما لو كانت مش
+
+154
+00:11:32,780 --> 00:11:37,420
+nano particles وفي نفس الوجد fixed على الأسطح
+
+155
+00:11:37,420 --> 00:11:40,580
+fixed يعني ثابتة مش لو مثلا بتروح مع الأسطح لأ
+
+156
+00:11:40,580 --> 00:11:47,060
+ثابتة وإن لو عندها قدرة قتل على ال .. قدرة قتل
+
+157
+00:11:47,060 --> 00:11:50,300
+للبكتيريا أو لل micro organism إيه ده؟ لأ مثلا
+
+158
+00:11:53,960 --> 00:11:57,940
+هو من ايه؟ من التلامس، إذا صراحة نعيش الآن on
+
+159
+00:11:57,940 --> 00:12:01,340
+antimicrobial surfaces، ياريت جوالاتنا في يوم من
+
+160
+00:12:01,340 --> 00:12:04,460
+الأيام ال touch هذي اللي بنستخدمها لكل حاجة،
+
+161
+00:12:04,460 --> 00:12:08,100
+بنستخدمها للاتصال، لل browsing، للإنترنت، لل
+
+162
+00:12:08,100 --> 00:12:11,920
+email، بعض ما بيدرس منها، بعض ما بيسمع فيها، لكن
+
+163
+00:12:11,920 --> 00:12:16,750
+في الآخر بنستخدم أحنا أصابعناواصبح هنا دائما أبدا
+
+164
+00:12:16,750 --> 00:12:19,890
+أن أقول هذه الجملة ولا أمل من تكرارها هي الأداة
+
+165
+00:12:19,890 --> 00:12:23,890
+الأكتر استخداما عند البشر وكونها الأداة الأكتر
+
+166
+00:12:23,890 --> 00:12:27,310
+استخداما فهي الأداة الأكتر هفضل التلوث وبالتالي
+
+167
+00:12:27,310 --> 00:12:31,430
+أنا بستخدمها جاد زي ما تخيلوا معاكم الشكل اللي
+
+168
+00:12:31,430 --> 00:12:36,150
+بتطلعيه من جيبتك تمام؟ اتخيل أيا كان واحد لمسه
+
+169
+00:12:36,150 --> 00:12:41,230
+ولمّا لمسه هذا الشخص هل كان في إيده نظيفة ولا مش
+
+170
+00:12:41,230 --> 00:12:42,110
+نظيفة تمام؟
+
+171
+00:12:44,760 --> 00:12:47,860
+طبعا هذا شكل لو مسكنها بإيدينا يبقى إيدينا فيهم
+
+172
+00:12:47,860 --> 00:12:52,300
+مشكلة استخدنا الجوال .. استخدنا الجوال .. فكرة
+
+173
+00:12:52,300 --> 00:12:56,200
+الباب اللى فتحناها ودخلناها ده كان واحد فتحها ..
+
+174
+00:12:56,200 --> 00:12:59,700
+كتير .. كانوا مستطيعين من الدورات من غير ما يغسلوا
+
+175
+00:12:59,700 --> 00:13:04,700
+إيديهم وفي الآخر إيدينا مسكت الجوال .. تمام؟
+
+176
+00:13:04,700 --> 00:13:11,560
+وحركنا فيه وعملنا .. فلو أنا مثلا .. mobile .. ال
+
+177
+00:13:11,560 --> 00:13:12,200
+cover تبعه
+
+178
+00:13:15,220 --> 00:13:20,580
+تكون antimicrobial surface بيكون احنا كده يعني
+
+179
+00:13:20,580 --> 00:13:23,120
+ضمننا على الأقل انه الميكروبات اللى بتيجي على
+
+180
+00:13:23,120 --> 00:13:27,440
+الجوال تبعنا ماتت طيب المقاوض اللى احنا اتكلمنا
+
+181
+00:13:27,440 --> 00:13:32,340
+عنها دى لو صنعت من النحاس و النحاس heavy metal و
+
+182
+00:13:32,340 --> 00:13:37,020
+احنا لبسناها و ادينا مش انضاف تمام الان الميكروبات
+
+183
+00:13:37,020 --> 00:13:40,180
+اللى كانت عدنا صارت على المقبض المقبض بيقتلها
+
+184
+00:13:48,000 --> 00:13:51,640
+في كل الأبواب أو الأسطح اللي ممكن الناس ..شوفوا
+
+185
+00:13:51,640 --> 00:13:55,560
+بتاع في بواب مالاش إيديان، زك زك، المكان اللي
+
+186
+00:13:55,560 --> 00:14:00,260
+بندفع منه الباب برضه مطلبي اللي هو النحاس حتى نقتل
+
+187
+00:14:00,260 --> 00:14:03,700
+ال micro organisms، إذا ال heavy metal وإن كان
+
+188
+00:14:03,700 --> 00:14:07,540
+زمان استخدامها يعني غير شائع إلا من ناحية اللي هو
+
+189
+00:14:13,020 --> 00:14:18,060
+لكن انا متأكد لو سألته الوالد او الوالدة هتلاقوا
+
+190
+00:14:18,060 --> 00:14:21,880
+انه بيعرفوه كويس microchrome صابغة حمرا صابغة
+
+191
+00:14:21,880 --> 00:14:26,540
+حمراء اللون كانت تستخدم بكثرة في تطهير الجروح لكن
+
+192
+00:14:26,540 --> 00:14:32,660
+نظرا لسمية الذئبق ووجود بدائل عديدة بعد ذلك تم
+
+193
+00:14:32,660 --> 00:14:35,740
+إيقافه فينا كمان حاجة اسمها silver nitrate
+
+194
+00:14:35,740 --> 00:14:38,400
+eyedrops جطرة عين
+
+195
+00:14:42,020 --> 00:14:48,440
+لحد يمكن أواخر السبعينات كانت كإجراء قياسي، كل أم
+
+196
+00:14:48,440 --> 00:14:51,700
+بتولد طفل، الطفل لازم يتجطرله في عينه silver
+
+197
+00:14:51,700 --> 00:14:54,880
+nitrate، ليش؟ لأنهم عندهم منتشر مرض اسمه نيسيريا
+
+198
+00:14:54,880 --> 00:14:58,580
+.. عفوا مرض الجنورية اللي بتسببه مرض نيسيريا
+
+199
+00:14:58,580 --> 00:15:02,690
+الجنوريةالجنين هو خارج عبر قناة الولادة بياخد من
+
+200
+00:15:02,690 --> 00:15:05,830
+الام نيسيريا جونرقية أحد الأموات اللي ممكن تصيب
+
+201
+00:15:05,830 --> 00:15:09,590
+الطفل الحديث الولادة هذا حادث ما أوبثالميا
+
+202
+00:15:09,590 --> 00:15:14,770
+نيوناتروم التهابات العين لدى الأطفال الموليد الجدد
+
+203
+00:15:14,770 --> 00:15:18,350
+نيوناتروم نيونيت يعني أوبثالميا ليها التهاب العين
+
+204
+00:15:19,280 --> 00:15:23,640
+فكان كإدراع قياسي إن كل طفل بيولد سواء أمه مصابة
+
+205
+00:15:23,640 --> 00:15:26,220
+أو غير مصابة، في الآخر ماما مش عارفين يعني، فكان
+
+206
+00:15:26,220 --> 00:15:29,680
+إدراع قياسي يحطه برضه قطرة لعين، بالظبط كده، الآن
+
+207
+00:15:29,680 --> 00:15:34,320
+استبدلوها ب Antibiotics لكن واضح من الكلام اللي
+
+208
+00:15:34,320 --> 00:15:38,960
+بنحكي إن ال heavy metals كان لها ماضي و لها
+
+209
+00:15:38,960 --> 00:15:42,500
+مستقبل، يعني نتكلم على ال antimicrobial surfaces
+
+210
+00:15:42,500 --> 00:15:46,140
+اللي احنا ذكرناها، خاصة لو وجود التقنية اللي اسمها
+
+211
+00:15:46,140 --> 00:15:50,780
+nanotechnologyفي الجيل الأخير في اللوحة اسمها
+
+212
+00:15:50,780 --> 00:15:55,140
+silver design is used to prevent infection of burn
+
+213
+00:15:55,140 --> 00:15:59,120
+wounds لو وقعنا على قسم الحروق في أي مستشفى في
+
+214
+00:15:59,120 --> 00:16:03,820
+الدنيا سواء محليا أو عالميا وسألناهم ما هي المشكلة
+
+215
+00:16:03,820 --> 00:16:08,820
+الأخطر على مرضى الحروق هقولك انه التهيبات الحروق
+
+216
+00:16:08,820 --> 00:16:14,400
+لماذا الجلب الذي كان يقوم بوظيفته كعازل كمان
+
+217
+00:16:24,900 --> 00:16:31,200
+أهم أولويات الطواقم انهم يمنعوا حدوث ال infection
+
+218
+00:16:31,200 --> 00:16:37,300
+بيضيفوا مواد زي silver dioxin to prevent انه يلو
+
+219
+00:16:37,300 --> 00:16:41,200
+antimicrobial action ايش ميزة ان انا استخدم
+
+220
+00:16:41,200 --> 00:16:47,200
+disinfectant او antiseptic من اللي يلهم اليوم
+
+221
+00:16:52,140 --> 00:16:55,680
+المشكلة أصلا في قسم الحروق هي Sedum Oras Erogenosa
+
+222
+00:16:55,680 --> 00:17:00,360
+و Sedum Oras Erogenosa من أكثر الميكروبات قدرة على
+
+223
+00:17:00,360 --> 00:17:03,800
+تكوين ال resistance تخيلوا في عزلات منها تعزل في
+
+224
+00:17:03,800 --> 00:17:10,160
+غزة هنا أن كلها مقاومة لكل المناطق الحيوية ففي هذا
+
+225
+00:17:10,160 --> 00:17:14,920
+الحالة كيف نقتلها كيف نمنعها لمراكبات من هذا القبر
+
+226
+00:17:14,920 --> 00:17:17,880
+هذا البروكسايت
+
+227
+00:17:22,180 --> 00:17:26,600
+و mercury لكن الكفر والزنك أيضا كمان لهم ضبابيادة
+
+228
+00:17:27,310 --> 00:17:30,930
+ال hydrogen peroxide درسناه لما .. لما شرحنا كلمة
+
+229
+00:17:30,930 --> 00:17:34,430
+oxygen toxicity فاكرينها؟ ال concept أن ال oxygen
+
+230
+00:17:34,430 --> 00:17:40,290
+toxic لنا لكن كونه أن موجود انزين كتاليز ساعدنا في
+
+231
+00:17:40,290 --> 00:17:43,410
+التخلص من الصميم تبع ال hydrogen peroxide اللي نجم
+
+232
+00:17:43,410 --> 00:17:48,970
+عن تكون شقوق حرة من ال oxygen اللي هي ال O singlet
+
+233
+00:17:48,970 --> 00:17:53,990
+تفارق مع الماء فكون H2O أن كتاليز ضيع تأثير دماغ
+
+234
+00:17:54,940 --> 00:17:58,980
+طيب بتوقع أن ال hydrogen peroxide يكون قاتل لمين؟
+
+235
+00:17:58,980 --> 00:18:03,780
+للبكتيرا الهوائية ولا اللاهوائية؟ اللاهوائية ليش؟
+
+236
+00:18:03,780 --> 00:18:07,360
+لأن البكتيرا اللاهوائية ما عندها كتاليز فما عندها
+
+237
+00:18:07,360 --> 00:18:11,080
+القدرة على أن ت counteract اللي هو ال effect بين
+
+238
+00:18:11,080 --> 00:18:14,360
+hydrogen peroxide بينما البكتيرا الهوائية فيها
+
+239
+00:18:14,360 --> 00:18:17,740
+كتاليزبما أن فيها كتاليز يمكن لو حطينا عليه
+
+240
+00:18:17,740 --> 00:18:21,420
+hydrogen peroxide هتحوله لمين؟ لميال وخوطى وهذا ال
+
+241
+00:18:21,420 --> 00:18:25,260
+principle تبقى فحص الكتاليز في المختبر بنجيب
+
+242
+00:18:25,260 --> 00:18:28,380
+البكتيريا يا بنات البكتيريا المجهولة بالنسبة لنا
+
+243
+00:18:28,380 --> 00:18:31,560
+بنحط قطرة من ال hydrogen peroxide على شريحة و
+
+244
+00:18:31,560 --> 00:18:35,880
+بنضيف البكتيريا باللوب على الشريحة إذا طلعت
+
+245
+00:18:35,880 --> 00:18:38,540
+bubbles فهي كتاليز مصطفى
+
+246
+00:18:41,140 --> 00:18:45,400
+يعني حولت الهيدرين بيروكسايت إلى ماء زائد أكسجين
+
+247
+00:18:45,400 --> 00:18:49,960
+الأكسجين هيطلع شكل بابايز من خلال قطرة الماء كيف
+
+248
+00:18:49,960 --> 00:18:52,140
+بيشتغل الهيدرين بيروكسايت؟ احنا بقول عنه هو
+
+249
+00:18:52,140 --> 00:18:57,100
+powerful oxidizing agent that attacks sulfhydryl
+
+250
+00:18:57,100 --> 00:18:59,920
+group thereby inhibiting enzymatic activity دي
+
+251
+00:18:59,920 --> 00:19:03,240
+تجابب التأثير الأساسي تبقى على البروتينات اللي من
+
+252
+00:19:03,240 --> 00:19:07,420
+ضمنها مين؟ الإنزينات وانت كلكوا بتعرفوا حتى يوم
+
+253
+00:19:07,420 --> 00:19:12,870
+اتمشروحنا احنا ال .. ال .. ال ..العوامل المؤثرة
+
+254
+00:19:12,870 --> 00:19:16,030
+على نموم الخلية و ذكرنا الحرارة و ذكرنا ال pH
+
+255
+00:19:16,030 --> 00:19:20,530
+ذكرناهم من أي ناحية بيبينكوا فيه minimum و maximum
+
+256
+00:19:20,530 --> 00:19:25,350
+و optimum لل enzymatic activity يعني اللي بتأثر في
+
+257
+00:19:25,350 --> 00:19:29,390
+الخلية و يقع الأمر و يجعل الخلية تموت هو تأثر ال
+
+258
+00:19:29,390 --> 00:19:34,110
+enzymes بال pH تأثر ال enzyme بالحرارة طبعا فلما
+
+259
+00:19:34,110 --> 00:19:37,110
+يكون مافيه enzyme يبقى فينا مشكلة في نموم الخلية
+
+260
+00:19:38,030 --> 00:19:40,790
+والخلية اللى وصفناها المرة الماضية الميتة لما
+
+261
+00:19:40,790 --> 00:19:46,750
+وصفنا الموت فى الخلية خلية غير قادرة على أن تتم ال
+
+262
+00:19:46,750 --> 00:19:49,650
+reproduction مع أنه قلتلكوا أنا يعني هذا مش
+
+263
+00:19:49,650 --> 00:19:52,950
+absolute term برضه لكن فى الآخر هذا اللى هو
+
+264
+00:19:52,950 --> 00:19:58,810
+المستخدم فى قياس نجاعة المراكبات المراقبة الأولى
+
+265
+00:19:58,810 --> 00:20:00,830
+هو ممكن ألاجى بكتيريا غير قادرة على أن تتم ال
+
+266
+00:20:00,830 --> 00:20:03,610
+reproduction وخلاها تتم ال reproduction بعد ذلك
+
+267
+00:20:03,610 --> 00:20:08,340
+عملها revivalطيب مركّب رقم 5 في السلسلة اللي هو ال
+
+268
+00:20:08,340 --> 00:20:12,040
+formaldehyde and glutalaldehyde أشفر حاجة في
+
+269
+00:20:12,040 --> 00:20:17,480
+الدنيا أن نروح على مختبر ال pathology في كورية
+
+270
+00:20:17,480 --> 00:20:22,880
+الطب نشم ريحة ال formalinو زمان بجعل نفسي من
+
+271
+00:20:22,880 --> 00:20:26,000
+الأحياء يستخدم ال formalin بكثرة فى التحنية فكنى
+
+272
+00:20:26,000 --> 00:20:30,640
+لما ندخل المختبر على طول أصبح لنا irritation أنا
+
+273
+00:20:30,640 --> 00:20:34,680
+شخصيا أقعد يومين أكح من ال .. ال .. ال .. الصنشاق
+
+274
+00:20:34,680 --> 00:20:39,280
+اللى هو ال fumes تبع ال .. ال formalin ال formalin
+
+275
+00:20:39,280 --> 00:20:44,420
+او اللى هو formaldehyde عبارة عن محلول بتركيز 37%
+
+276
+00:20:44,420 --> 00:20:48,890
+من الواقعإذا .. إذا كان هذا المحلول ال formal ده
+
+277
+00:20:48,890 --> 00:20:51,710
+هيتحلل أبدا بيبقى بتركيز مسمّيه الآن إيه؟ Formalin
+
+278
+00:20:51,710 --> 00:20:59,090
+Formalin بيستخدم لحفظ كلمة حفظ preserve إيش معنى
+
+279
+00:20:59,090 --> 00:21:03,050
+preserve؟ تحنية زي مش مهم بحنطهم بال .. بالحياة
+
+280
+00:21:03,050 --> 00:21:07,070
+بال .. بالformalin أو الأجنة أو الأعضاء إيش
+
+281
+00:21:07,070 --> 00:21:11,550
+معناها؟ معناها إنه مافيش biological activity
+
+282
+00:21:11,550 --> 00:21:15,680
+biological activityطب مافيه بكتيريا، البكتيريا
+
+283
+00:21:15,680 --> 00:21:19,720
+كمان صارها preservation، البكتيريا نفسها ماتت،
+
+284
+00:21:19,720 --> 00:21:24,540
+قُتلت، فالformalin بيقتل الخلايا، تعالى نطلع على
+
+285
+00:21:24,540 --> 00:21:28,460
+ال .. على ال .. ال .. ال action تبعها، ماعرفش
+
+286
+00:21:28,460 --> 00:21:35,080
+موجود ال action، اللي هيتقن، ال .. both proteins
+
+287
+00:21:35,080 --> 00:21:39,380
+and nucleic acid contain essential NH2، اللي ال
+
+288
+00:21:39,380 --> 00:21:41,420
+amino group and hydroxyl group
+
+289
+00:21:46,760 --> 00:21:50,020
+الـ two groups اللي هم ال amino group زائد ال OH
+
+290
+00:21:50,020 --> 00:21:54,260
+group are the main site of alkylation يعني ال
+
+291
+00:21:54,260 --> 00:21:58,720
+formalin بيعمل alkylation لل proteins و ال DNA عبر
+
+292
+00:21:58,720 --> 00:22:04,120
+ارتباط مع مين؟ مع ال NH2 و مع ال OH، by the
+
+293
+00:22:04,120 --> 00:22:07,380
+hydroxy methyl group of formaldehyde يعني ال
+
+294
+00:22:07,380 --> 00:22:09,800
+formaldehyde بيستخدم ال group هيتبعته في الارتباط
+
+295
+00:22:09,800 --> 00:22:17,310
+مع ال OH و ال NHبهذه العملية بيعمل destruction
+
+296
+00:22:17,310 --> 00:22:21,090
+للبروتين أو بالأحرى denaturation للبروتين و
+
+297
+00:22:21,090 --> 00:22:25,090
+denaturation للمادة الوراثية انا الان بتكلم على كل
+
+298
+00:22:25,090 --> 00:22:30,350
+البروتين اللي من ضمنها ال autolytic enzymes تعرفوا
+
+299
+00:22:30,350 --> 00:22:32,630
+ان في الخلايا تبعتنا في autolytic enzymes اللي هي
+
+300
+00:22:32,630 --> 00:22:35,670
+الخلايا الغاضي ماداتية تخضر نفسها، امام؟
+
+301
+00:22:35,670 --> 00:22:41,680
+والبكتيريا فكل الخلايا وسيلها preservation وقتللكن
+
+302
+00:22:41,680 --> 00:22:45,720
+زي ما ذكرتلكوا الفورمالين او ال formaldehyde يصعب
+
+303
+00:22:45,720 --> 00:22:49,720
+استخدامه انه انطهر فيه ارضيات او اسطح لانه بيعمل
+
+304
+00:22:49,720 --> 00:22:55,120
+irritation يليه اخه اسمه glutaraldehydeطالع لو
+
+305
+00:22:55,120 --> 00:22:58,820
+gluteal aldehyde بيفرج عنه بيقولك انه يمتلك two
+
+306
+00:22:58,820 --> 00:23:03,320
+reactive aldehyde group مش هو formaldehyde من
+
+307
+00:23:03,320 --> 00:23:05,400
+مولود aldehyde group, gluteal aldehyde من مولود
+
+308
+00:23:05,400 --> 00:23:08,340
+aldehyde group هذا بيمتلك ايه؟ two aldehyde group
+
+309
+00:23:08,340 --> 00:23:12,900
+it is ten times more effective than formaldehyde
+
+310
+00:23:12,900 --> 00:23:18,220
+عشرة مرات اكتر و يستخدم في ال disinfection ل
+
+311
+00:23:18,220 --> 00:23:22,510
+buildings ايش يعني buildings؟تخيلوا أنه مابنا من
+
+312
+00:23:22,510 --> 00:23:25,810
+المباني صار فيه وباء أو اتشافنا مرض طعو ابولا ..
+
+313
+00:23:25,810 --> 00:23:28,830
+تسمعوا ابولا ال .. ال .. ال .. ال .. ال .. ال ..
+
+314
+00:23:28,830 --> 00:23:29,150
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+315
+00:23:29,150 --> 00:23:29,170
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+316
+00:23:29,170 --> 00:23:29,490
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+317
+00:23:29,490 --> 00:23:30,010
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+318
+00:23:30,010 --> 00:23:30,370
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+319
+00:23:30,370 --> 00:23:33,550
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+320
+00:23:33,550 --> 00:23:33,750
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+321
+00:23:33,750 --> 00:23:33,850
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+322
+00:23:33,850 --> 00:23:33,990
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+323
+00:23:33,990 --> 00:23:37,630
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+324
+00:23:39,860 --> 00:23:43,360
+بيدخلوا المختصين اللى بتشوفهم لابسين زى الفضاء
+
+325
+00:23:43,360 --> 00:23:46,980
+عشان ما يظهرش عندهم اى دخول ال micro organism
+
+326
+00:23:46,980 --> 00:23:52,160
+ويتنفسوا عبر خراطيم وانبوب من ال oxygen و بيسكروا
+
+327
+00:23:52,160 --> 00:23:55,580
+كل المنافذ بيعملوا sealing لكل المنافذ اللى موجودة
+
+328
+00:23:55,580 --> 00:23:58,940
+بالورفة و بعدين بيطلقوا بيعملوا فتحة و بيطلقوا غاز
+
+329
+00:23:58,940 --> 00:24:02,100
+اللى هو ال formaldehyde .. عفوا ال glutalaldehyde
+
+330
+00:24:02,100 --> 00:24:05,260
+لعمليةthis infection اللي بتروح طبعا بتروح كل فترة
+
+331
+00:24:05,260 --> 00:24:10,920
+ممكن تتصل لأيام و بعدين بيسحبوا بيفرغوا اللي هو
+
+332
+00:24:10,920 --> 00:24:16,260
+الغاز و بترجع الغرفة لطبيعة طبعا غاز بدخل إلى
+
+333
+00:24:16,260 --> 00:24:22,880
+أناكن لا يستطيع دخول إلى السائل صح ولا لأ و قدرته
+
+334
+00:24:22,880 --> 00:24:27,120
+على يعني خدنا نقول القتل قولنا عنها عالي جدا أعلى
+
+335
+00:24:27,120 --> 00:24:31,680
+من مين من اللي هو ال ..اللي هو استخدامه أيضا في
+
+336
+00:24:31,680 --> 00:24:34,840
+المستشفيات انه بيستخدموه لتعقيم ال respiratory
+
+337
+00:24:34,840 --> 00:24:38,420
+equipments، الأجنزة اللي .. اللي .. اللي بتوظف
+
+338
+00:24:38,420 --> 00:24:41,260
+فيه، اللي .. اللي .. اللي بتلفظ، لما واحد
+
+339
+00:24:41,260 --> 00:24:43,980
+بيستخدمها وبعدين بيستخدمها الأول و واحد تاني،
+
+340
+00:24:43,980 --> 00:24:46,140
+الأولاني ما هو أصلا ممكن يكون عنده micro
+
+341
+00:24:46,140 --> 00:24:50,780
+-orientalي، بدنا نعقمها بهذا الطريق أساس مبتوء انه
+
+342
+00:24:50,780 --> 00:24:54,600
+less toxic؟ أقل ثومي نعمعشان هيك احنا بيقول إن
+
+343
+00:24:54,600 --> 00:24:58,480
+استخدامه أكتر شيوعا في ال disinfection للأماكن،
+
+344
+00:24:58,480 --> 00:25:02,380
+يعني لو صار leakage، تسريب منه، بيبقى لسه أقل، أقل
+
+345
+00:25:02,380 --> 00:25:05,960
+صينية بس برضه حكيت إنه لما بيستخدموه بيعملوا كل
+
+346
+00:25:05,960 --> 00:25:06,320
+اللي ..
+
+347
+00:25:32,120 --> 00:25:34,680
+الغاز الأشهر على الإطلاق في التعقيم
+
+348
+00:25:37,180 --> 00:25:42,260
+أدى حد مشاكله أنه بده containment يعني حجرة ده
+
+349
+00:25:42,260 --> 00:25:42,660
+مواصفاته
+
+350
+00:25:50,090 --> 00:25:53,210
+مصمم بطريقة معينة بحيث انه مايزالش فيه اختراق لكن
+
+351
+00:25:53,210 --> 00:25:58,950
+في العصر الحديد اللي احنا فيه الان تم تصميم اجهزة
+
+352
+00:25:58,950 --> 00:26:03,530
+صغيرة يعني بدل الغرفة الكبيرة زي جهاز ال autoclave
+
+353
+00:26:03,530 --> 00:26:07,790
+او ال oven اللي تكون desktop و غير قابل الاختراق
+
+354
+00:26:07,790 --> 00:26:12,420
+من قبل هذا الجهازلكن ميزته زي ما حكينا انه بيعقم
+
+355
+00:26:12,420 --> 00:26:16,400
+أشياء لا يمكن تعقيمها بالحرارة فال heat sensitive
+
+356
+00:26:16,400 --> 00:26:19,860
+material خاصة المواد الجراحية أو الأشياء الجراحية
+
+357
+00:26:19,860 --> 00:26:23,180
+اللي يمكن تأثر بالحرارة أو الأشياء اللي بلاستيكية
+
+358
+00:26:23,180 --> 00:26:28,940
+بنعقيمها بمين؟ بالethylene oxide هذا عبارة عن
+
+359
+00:26:28,940 --> 00:26:31,900
+alkalating agent و بيعمل alkalation برضه لل
+
+360
+00:26:31,900 --> 00:26:37,500
+protein زي اللي هو مين؟ ال ال nucleic acidsنجي
+
+361
+00:26:37,500 --> 00:26:48,360
+للاخير في السلسلة الاسد الأحمار
+
+362
+00:26:48,360 --> 00:26:55,940
+والقواعد بنجسبها لجسمين للي عندها pH منخفضبيتش
+
+363
+00:26:55,940 --> 00:27:01,540
+مقفض يعني high acidity اقوله acidity ال strong
+
+364
+00:27:01,540 --> 00:27:04,780
+acids اللي هي الأحماض القوية زي ال sulfuric و ال
+
+365
+00:27:04,780 --> 00:27:10,260
+hydrochloric they kill by denaturing proteins عن
+
+366
+00:27:10,260 --> 00:27:13,400
+طريق اللي هو تدمير ال protein انا بدا بس بدي
+
+367
+00:27:13,400 --> 00:27:19,360
+اعطيكوا شغل لطيف يعني لما احنا بنحرق البكتيريا
+
+368
+00:27:19,360 --> 00:27:27,240
+ماهي ميكانيزم تبعت القتلهي دمرها .. كلهم بدمروا ال
+
+369
+00:27:27,240 --> 00:27:31,780
+bakteria لكن كل واحدة منهم انتقلالها مصطلح كنا
+
+370
+00:27:31,780 --> 00:27:35,580
+بتعمل denaturation لكذا او لكذا فيردت يعني ان
+
+371
+00:27:35,580 --> 00:27:37,580
+تعطونا تعبير مناسب للحرق
+
+372
+00:27:53,610 --> 00:27:56,730
+صح هذا لأ؟ ومن ضمنها ال .. من ضمنها ال cell
+
+373
+00:27:56,730 --> 00:27:59,510
+membrane و ال cell wall و ال proteins و كل شيء
+
+374
+00:27:59,510 --> 00:28:03,730
+يبقى فيه عملية oxidation و تاعة بقول strong acid
+
+375
+00:28:03,730 --> 00:28:07,290
+غالبا بيعمل denaturation لمحطة المركبات سواء كانت
+
+376
+00:28:07,290 --> 00:28:11,770
+البروتينات أو غير المركبات حتى إيه ده؟ انتوا بعرفش
+
+377
+00:28:11,770 --> 00:28:15,950
+شفتوا sulfuric acid مركز و .. و .. و hydrofluoric
+
+378
+00:28:15,950 --> 00:28:19,650
+acid مركز لو حطيناه على الأرض إيش بيعمل؟ على
+
+379
+00:28:19,650 --> 00:28:25,110
+الطربة أو على بلاطبيثقوا ال .. البلاط، ال ..
+
+380
+00:28:25,110 --> 00:28:30,930
+الرخام، تمام؟ بيدوبوا، فبالتالي قدرته على القتل زي
+
+381
+00:28:30,930 --> 00:28:34,910
+الحرق، لأنه strong acid، لأنه strong acid طبعا هذا
+
+382
+00:28:34,910 --> 00:28:38,090
+بالاضافة إلى أنه، إيش بتكلم عنه هنا؟ عن low pH،
+
+383
+00:28:38,090 --> 00:28:43,790
+low pH دائما و أبدا في استثناءات، بتاع من
+
+384
+00:28:43,790 --> 00:28:46,710
+mycobacterium tuberculosis، حكينا عنها قبل اللي
+
+385
+00:28:46,710 --> 00:28:48,550
+بتسبب tuberculosis
+
+386
+00:28:52,690 --> 00:28:56,470
+ال micro bacterium tuberculosis عندها مقدرة غير
+
+387
+00:28:56,470 --> 00:28:59,330
+طبيعية على مقاومة ال acid and alkaline أعطيكم
+
+388
+00:28:59,330 --> 00:29:04,330
+إدهار، المريض لما بيكح و بيطلع البكتيريا بعض
+
+389
+00:29:04,330 --> 00:29:07,470
+الأحيان بيطلعش لشوية من برا بيبلعو، فده أمر طبيعي،
+
+390
+00:29:07,470 --> 00:29:10,630
+يعني الكل حتى اللي مش عنده tuberculosis لما بيكون
+
+391
+00:29:10,630 --> 00:29:14,630
+نايم و بيكح و بيطلع شوية من غالبا بيبلعو، البلع
+
+392
+00:29:14,630 --> 00:29:21,180
+هذا بيروح المعيدة، المعيدةال pH تبعها إيه؟ مخفض،
+
+393
+00:29:21,180 --> 00:29:24,820
+غالبا بتقتل معظم الميكروبات، لكن لو أخدنا .. لو
+
+394
+00:29:24,820 --> 00:29:28,680
+أحنا من جديد ناخد من مريض tuberculosis عينة Sputum
+
+395
+00:29:28,680 --> 00:29:32,940
+ممكن ناخد gastric lavage، ناخد غسيل المعدة، و نازل
+
+396
+00:29:32,940 --> 00:29:35,420
+البكتيرا، مانا الحاجة للبكتيرا شمالها، مقاومة
+
+397
+00:29:35,420 --> 00:29:41,080
+لمين؟ للاسد، و في المعمل لما بناخد عينة Sputum،
+
+398
+00:29:41,080 --> 00:29:46,360
+بنروح بنحط على Sputum 2% Sodium Hydroxide، الغرض
+
+399
+00:29:46,360 --> 00:29:50,470
+منه شغل تانيالغرض الأول ان بنعمل liquefaction
+
+400
+00:29:50,470 --> 00:29:53,890
+للاسموتام، الاسموتام بيكون قوامه لزج، البكتيريا
+
+401
+00:29:53,890 --> 00:29:58,510
+بتكون مرتبطة بخيوط الميوكاس، فبنحلها على الميوكاس
+
+402
+00:29:58,510 --> 00:30:02,530
+و بنحط اللي هو مركب للإيماها، إيماها يعني جعله شكل
+
+403
+00:30:02,530 --> 00:30:06,130
+سائل زي المي يعني، فبنحط انيواتش، بنفس الواجهة
+
+404
+00:30:06,130 --> 00:30:09,860
+معظم البكتيريا بتموت من الانيواتش، معناه؟الـ
+
+405
+00:30:09,860 --> 00:30:13,880
+Mycobacterium tuberculosis فهو عملية disinfection
+
+406
+00:30:13,880 --> 00:30:17,980
+يعني فتغيير لـ Sputum و في نفس الوقت إيماها لتحليل
+
+407
+00:30:17,980 --> 00:30:22,260
+البكتيريا من مين؟ من خيوط الـ Sputum كمان مرهض
+
+408
+00:30:22,260 --> 00:30:27,880
+للموضوع على مين؟ على exception ذكرناه في معرض
+
+409
+00:30:27,880 --> 00:30:31,200
+حديثنا عن مين؟ على الـ strong acid and alkaline
+
+410
+00:30:31,200 --> 00:30:39,740
+طيب ال weak acids اللي هي الأحماض الضعيفةواللي
+
+411
+00:30:39,740 --> 00:30:44,080
+مشهور منها الـ Benzoic Acid و ال Acetic Acid و ال
+
+412
+00:30:44,080 --> 00:30:46,440
+Propionic Acid و ال Citric Acid اللي هو حمض اللمون
+
+413
+00:30:46,440 --> 00:30:51,060
+المشهور are frequently used as food preservatives
+
+414
+00:30:51,060 --> 00:30:54,540
+ايش يعني food preservatives؟ مواد حافظة للأرض
+
+415
+00:30:54,540 --> 00:30:58,620
+because they are bacteriostatic مايعني
+
+416
+00:30:58,620 --> 00:31:03,900
+bacteriostatic؟ bacteriostatic عكسها bactericidal
+
+417
+00:31:07,670 --> 00:31:15,690
+قالولي بيوقف استاتيك مطبط للنمو بينما الصيدل قاتل
+
+418
+00:31:15,690 --> 00:31:22,470
+للبكتيريا الان
+
+419
+00:31:22,470 --> 00:31:27,270
+ال weak acids بتشتغل طول ابتدا ان نفس ال organic
+
+420
+00:31:27,270 --> 00:31:34,140
+ميتي يعني الجزء العضوي فيه ال organic acidاللي هو
+
+421
+00:31:34,140 --> 00:31:37,400
+قدرة على Antibacterial زي ال Antibiotics، يعني
+
+422
+00:31:37,400 --> 00:31:44,000
+بيمنع steps معينة فيه البكتيريا، بالإضافة إلى أنه
+
+423
+00:31:44,000 --> 00:31:48,540
+بيخفض منين؟ ال pH، بيخفض ال pH، إذا عندنا ال acid
+
+424
+00:31:48,540 --> 00:31:53,200
+والalkali ممكن يستخدموا كمواد قاتلة أو مضابطة لنهو
+
+425
+00:31:53,200 --> 00:31:57,250
+البكتيريااللوحة اللي .. اللي .. اللي أمامنا الآن
+
+426
+00:31:57,250 --> 00:32:01,070
+هي المعبرة أو اللي .. اللي فيها تعبير عن ال
+
+427
+00:32:01,070 --> 00:32:06,270
+modification of DNA أو المادة اللي وراها فيها و
+
+428
+00:32:06,270 --> 00:32:10,350
+الحقيقة أن احنا مش ذاكرين فيها إلا عن الصبغات
+
+429
+00:32:10,350 --> 00:32:15,490
+صبغتين مروا عليكم أو على الأقل واحدة منهم مرت
+
+430
+00:32:15,490 --> 00:32:20,870
+عليكم ليها ال crystal violetالcrystal white
+
+431
+00:32:20,870 --> 00:32:23,470
+استخدمته في ال gram stain لصفاة البكتيريا، اللي
+
+432
+00:32:23,470 --> 00:32:27,310
+الكلام اللي اتكلم عنه مختلف تماما، ليس له علاقة
+
+433
+00:32:27,310 --> 00:32:32,090
+بالصفاة، وإنما بعلاقة القتل وإيقاف النشاط، أنا
+
+434
+00:32:32,090 --> 00:32:37,170
+ذكرتلكوا إن ال Makunky أجار هو selective for gram
+
+435
+00:32:37,170 --> 00:32:41,730
+negative بكتيريا، بمعنى إنه بيقتل ال gram booster،
+
+436
+00:32:41,730 --> 00:32:46,470
+عارفين كيف قتل ال gram booster؟ أضفنا عليه small
+
+437
+00:32:46,470 --> 00:32:51,170
+concentration of crystal whiteبالاضافة إلى مادة
+
+438
+00:32:51,170 --> 00:32:53,610
+تانية، حنتعلمها بعدين، بس خلّيني أتكلم أقان عن
+
+439
+00:32:53,610 --> 00:32:57,570
+الصبرة ال crystal violet قتلت مين؟ قتلت ال gram
+
+440
+00:32:57,570 --> 00:33:00,650
+-positive at low concentration at higher
+
+441
+00:33:00,650 --> 00:33:03,370
+concentration they will kill both gram-positive
+
+442
+00:33:03,370 --> 00:33:06,090
+and gram-negative وهذا الكلمة بنفهمه إنه it is
+
+443
+00:33:06,090 --> 00:33:09,130
+dose dependent، إيش يعني dose dependent؟ يعني
+
+444
+00:33:09,130 --> 00:33:12,250
+معتمدة على الجرعة، والجرعة عبارة عن concentration
+
+445
+00:33:12,250 --> 00:33:15,130
+يعني بنقول الجرعة خمسة جرعة أو عشرة جرعة، فهي
+
+446
+00:33:15,130 --> 00:33:20,030
+concentration، dose dependentلكن ال crystal violet
+
+447
+00:33:20,030 --> 00:33:24,990
+لم نستخدمه لتغيير القصد و عزيزي المثال لإنه بيترك
+
+448
+00:33:24,990 --> 00:33:30,170
+stain تخيل أولا نظف الرخام في المستشفى و نصير ال
+
+449
+00:33:30,170 --> 00:33:31,750
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+450
+00:33:31,750 --> 00:33:33,370
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+451
+00:33:33,370 --> 00:33:34,170
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+452
+00:33:34,170 --> 00:33:34,170
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+453
+00:33:34,170 --> 00:33:34,690
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+454
+00:33:34,690 --> 00:33:37,430
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+455
+00:33:37,430 --> 00:33:37,930
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+456
+00:33:37,930 --> 00:33:37,930
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+457
+00:33:37,930 --> 00:33:37,930
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+458
+00:33:37,930 --> 00:33:41,270
+ال .. ال .. ال .. ال .. ال .. ال
+
+459
+00:33:44,140 --> 00:33:47,360
+لدرجة استخدامته محدودة، كان له استخدام طبي و
+
+460
+00:33:47,360 --> 00:33:51,100
+تقريبا وقف، كان بيستخدموه ك skin antiseptic، يعني
+
+461
+00:33:51,100 --> 00:33:55,040
+مطهر للجلود، و بالأخرى كان يستخدموه في مرض اسمه
+
+462
+00:33:55,040 --> 00:33:59,320
+oral trashبتعرف الحم اللى بيطلع فى الفم و بيكون
+
+463
+00:33:59,320 --> 00:34:05,480
+لون الفم أبيض خاصة عند الأطفال الرضع اللى بيفقد
+
+464
+00:34:05,480 --> 00:34:09,980
+القدرة على أو بيفقد الشهية على أنهم يرضع تمام؟ و
+
+465
+00:34:09,980 --> 00:34:13,340
+بيكون السبب فى ذلك أن الفم عنده فيه التهابات
+
+466
+00:34:13,340 --> 00:34:16,600
+فكانوا يستخدموه لهم هى to prevent secondary
+
+467
+00:34:16,600 --> 00:34:19,060
+infection و to treat candida albic يعني هى عبارة
+
+468
+00:34:19,060 --> 00:34:23,210
+عن خميرةلكنها الاستخدام مع وجود antifungal زي ال
+
+469
+00:34:23,210 --> 00:34:26,270
+نستاتين او تقولها النستاتين تقريبا وقف استخدامه
+
+470
+00:34:26,270 --> 00:34:31,670
+اسمه الشائع في السوق في صدليات jinshan fire و لحد
+
+471
+00:34:31,670 --> 00:34:36,170
+الان موجود و البعض بيستخدمه لنفس الغرض اللي انا
+
+472
+00:34:36,170 --> 00:34:43,870
+ذكرتكيف يقتل الصبغة .. كيف تقتل البكتيريا؟ simply
+
+473
+00:34:43,870 --> 00:34:48,610
+الصبغة كلكم عارفين أنها positively charged لأن
+
+474
+00:34:48,610 --> 00:34:51,550
+البكتيريا كانت negatively charged صح؟ و هنا بيرتبط
+
+475
+00:34:51,550 --> 00:34:54,510
+مع ال negative charge اللي موجودة في مين؟ في
+
+476
+00:34:54,510 --> 00:34:57,570
+الفوسفات .. مجموعة الفوسفات اللي موجودة في ال
+
+477
+00:34:57,570 --> 00:35:01,570
+backbone تبع اللي هو المادة البرادية لذلك انا قلنا
+
+478
+00:35:01,570 --> 00:35:04,690
+بيعمل modification لمين؟ لل نوكليك أسد .. لل
+
+479
+00:35:04,690 --> 00:35:10,980
+نوكليك أسد الملاكايت الجرين is anotherملاكايت جرين
+
+480
+00:35:10,980 --> 00:35:19,480
+ملاكايت جرين ملاكايت
+
+481
+00:35:19,480 --> 00:35:21,800
+جرين ملاكايت جرين ملاكايت جرين ملاكايت جرين
+
+482
+00:35:21,800 --> 00:35:22,320
+ملاكايت جرين ملاكايت جرين ملاكايت جرين ملاكايت
+
+483
+00:35:22,320 --> 00:35:22,480
+جرين ملاكايت جرين ملاكايت جرين ملاكايت جرين
+
+484
+00:35:22,480 --> 00:35:22,480
+ملاكايت جرين ملاكايت جرين ملاكايت جرين ملاكايت
+
+485
+00:35:22,480 --> 00:35:22,500
+جرين ملاكايت جرين ملاكايت جرين ملاكايت جرين
+
+486
+00:35:22,500 --> 00:35:22,500
+ملاكايت جرين ملاكايت جرين ملاكايت جرين ملاكايت
+
+487
+00:35:22,500 --> 00:35:24,220
+جرين ملاكايت جرين ملاكايت جرين ملاكايت جرين
+
+488
+00:35:24,220 --> 00:35:27,120
+ملاكايت جرين
+
+489
+00:35:32,330 --> 00:35:36,130
+الأولانية كانت مع الما كونكي صح؟ هذه بنحطها مع وسط
+
+490
+00:35:36,130 --> 00:35:40,310
+زراعي اسمه Leinstein-Jensen-Agar Leinstein-Jensen
+
+491
+00:35:40,310 --> 00:35:44,490
+-Agar وهو وسط selective للmycobacterium
+
+492
+00:35:44,490 --> 00:35:48,590
+tuberculosis الشهيرة mycobacterium tuberculosis
+
+493
+00:35:48,590 --> 00:35:51,910
+أرجو أنه ناخد وقتنا في هذا الموضوع
+
+494
+00:35:55,310 --> 00:36:01,090
+الmicrobacterium tuberculosis عملناها ب 2% NIH قبل
+
+495
+00:36:01,090 --> 00:36:06,310
+شوية، صح؟ ليش عملناها؟ عشان نقتل كل ما حولها من
+
+496
+00:36:06,310 --> 00:36:11,850
+الميكروبات، تمام؟ و نقيها هي حقيقة، ليش أنا خايف
+
+497
+00:36:11,850 --> 00:36:16,830
+عليها من الأخريات؟ أول حاجة اللي بدى نعزلها بطريقة
+
+498
+00:36:16,830 --> 00:36:22,080
+pure cultureرقم اتنين، فيه لها خاصية البكتيريا دي
+
+499
+00:36:22,080 --> 00:36:26,220
+بطيقة في النمو، كل عشرين ساعة بتنقصر، تخيل لو
+
+500
+00:36:26,220 --> 00:36:30,340
+حاطناها في وسط مع بكتيريا أخرى زي ال E.coli، ابتد
+
+501
+00:36:30,340 --> 00:36:33,440
+كاتر كل عشرين دقيقة، هيبقى الأكل إليها، الشيطان
+
+502
+00:36:33,440 --> 00:36:36,460
+إليها نوترين، يبقى معنا الحكي لن تلموا البكتيريا
+
+503
+00:36:36,460 --> 00:36:38,780
+في ورود البكتيريا الأخرى، لذلك لها يجب أن تكون
+
+504
+00:36:38,780 --> 00:36:43,950
+لوحدهافحاولت إن أنظفها من ال .. في العينة و لو
+
+505
+00:36:43,950 --> 00:36:49,510
+تبقى شيء لما بعد ما زلعتها بدي وسط .. بدي وسط يمنع
+
+506
+00:36:49,510 --> 00:36:52,390
+نمو أي مايكروبات فقط ال micro bacterium
+
+507
+00:36:52,390 --> 00:36:57,790
+tuberculosis اللي تنمو وضعنا ملكاية جرين في الوسط
+
+508
+00:36:57,790 --> 00:37:01,250
+لها دورة لقتل أي مايكروب
+
+509
+00:37:13,550 --> 00:37:18,170
+عشان تنقل البكتيريا في شكل مستعمرات بينها شهر و نص
+
+510
+00:37:19,290 --> 00:37:23,890
+فلو كان أي ميكروب في تلك الفترة موجود أكيد حينما
+
+511
+00:37:23,890 --> 00:37:27,970
+هو لو لا وجود الملكاية green and other components،
+
+512
+00:37:27,970 --> 00:37:31,690
+إذن كويس إن أحنا ساعدنا الداعيز في إنه نعمل
+
+513
+00:37:31,690 --> 00:37:34,290
+inhibition لبعض الميكروبات في صناعة الواء ال
+
+514
+00:37:34,290 --> 00:37:40,610
+selective يجي للجزء التاني من ال .. من ال .. ال ..
+
+515
+00:37:40,610 --> 00:37:42,410
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+516
+00:37:42,410 --> 00:37:42,650
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+517
+00:37:42,650 --> 00:37:43,010
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+518
+00:37:43,010 --> 00:37:43,630
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+519
+00:37:43,630 --> 00:37:44,190
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+520
+00:37:44,190 --> 00:37:44,350
+.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال ..
+
+521
+00:37:44,350 --> 00:37:44,830
+ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال
+
+522
+00:37:44,830 --> 00:37:53,380
+.. الأرجو انكوا تركزوا معايا الآن في الحديث طبعا
+
+523
+00:37:53,380 --> 00:37:56,780
+كل الماء اقوله غالبا هتكون مولا لوحات اللاحقة لكن
+
+524
+00:37:56,780 --> 00:38:00,880
+بدنا اجنح وننجي اللوحات لأ بدنا نركز في الموضوع
+
+525
+00:38:00,880 --> 00:38:06,200
+كمفهوم شامل ونشتغل على طريقة شوية مختلفة كلكوا
+
+526
+00:38:06,200 --> 00:38:15,780
+استخدمتوا الماء المغلي لتطهير او تعقيمأو تقليل عن
+
+527
+00:38:15,780 --> 00:38:19,800
+الميكروبات لكن موالة واحد فيكم بيقدر يقول ان انا
+
+528
+00:38:19,800 --> 00:38:24,400
+استخدمت الماء المغلي للتعقيد يعني الماء المغلي لا
+
+529
+00:38:24,400 --> 00:38:28,740
+يُعقِد الماء المغلي لا يُعقِد السبب ان هناك
+
+530
+00:38:28,740 --> 00:38:33,600
+endospores عند المقطع تحمل الحرارة اللي هي المئة
+
+531
+00:38:33,600 --> 00:38:37,780
+درجة موية بكل يسر وسهولة او تالي الماء المغلي لا
+
+532
+00:38:37,780 --> 00:38:41,830
+يُعقِد كنتوا في المختبر مساكتوا ال loopو ال needle
+
+533
+00:38:41,830 --> 00:38:46,770
+و حاططوها على اللهب و حرقتوها و بعدين بردتوا عشرين
+
+534
+00:38:46,770 --> 00:38:50,750
+ثانية تتذكروا الحركة حرق incineration ال
+
+535
+00:38:50,750 --> 00:38:55,170
+incineration عمل أكسر البكتيريا تماما كل مركبتها و
+
+536
+00:38:55,170 --> 00:39:00,630
+مكونتها هذا sterilization استخدمت حرارة و هذا
+
+537
+00:39:00,630 --> 00:39:06,110
+حرارة مش حرارة تانية حرارة عقمت و حرارة أخرى خفت
+
+538
+00:39:06,110 --> 00:39:10,840
+عدد البكتيريا لكن لم تحققإذن لما بنتكلم عن الحرارة
+
+539
+00:39:10,840 --> 00:39:14,540
+خدوا بالك الحرارة كمان لناخدها من جانب تاني طاقة
+
+540
+00:39:14,540 --> 00:39:20,240
+التسخين بيتطلب كهربا بيتطلب غاز فهو طاقة إذن برضه
+
+541
+00:39:20,240 --> 00:39:24,200
+كنت اقول انه من البداية ان عمليات التعقيم
+
+542
+00:39:24,200 --> 00:39:29,000
+المستخدمة للحرارة هي مواد أو عمليات مستهلكة للطاقة
+
+543
+00:39:29,000 --> 00:39:36,680
+كفائتها مختلفة استخداماتها مختلفة تسمع بالبس كلمة؟
+
+544
+00:39:37,600 --> 00:39:44,420
+البستر رشعت كمسمة لمن؟ للحليب، للحليب، يعني حليب
+
+545
+00:39:44,420 --> 00:39:49,340
+وبستر، لكن الحقيقة أن العصارى الكل اللي بتشربها
+
+546
+00:39:49,340 --> 00:39:54,620
+غالبا محفوظة بطريقة البستر، يعني مش للحليب بس،
+
+547
+00:39:54,620 --> 00:40:00,380
+والفكرة فيها رفع درجة الحرارة لحد معين، لزمن معين
+
+548
+00:40:00,380 --> 00:40:06,660
+ومن ثم خفضة سريعليش خطة سريعة؟ حتى لأسمح بال
+
+549
+00:40:06,660 --> 00:40:10,440
+adaptation .. إيش ال adaptation؟ إنه التكييف
+
+550
+00:40:10,440 --> 00:40:14,600
+للبكية، إذاً عملية نوع من الصدمة، حرارة عادة من
+
+551
+00:40:14,600 --> 00:40:19,680
+المفاجأة، مفيش عملية اللي واهي adaptation شوفنا
+
+552
+00:40:19,680 --> 00:40:23,900
+عمليات تسخين طب إن فئات الطبية في المستشفيات
+
+553
+00:40:23,900 --> 00:40:28,020
+بيلموها و بيحطوها في محرجة اسمة incinerator
+
+554
+00:40:34,860 --> 00:40:39,800
+الحرق هذا بيعقم بيقتل كل الميكروبات اللي موجودة
+
+555
+00:40:39,800 --> 00:40:44,820
+بال Oxidation طيب في المختبر لما بيحضروا الكوميديا
+
+556
+00:40:44,820 --> 00:40:48,400
+أو ساطر زراعية بيعقموها وين؟ بيعقموها بال
+
+557
+00:40:48,400 --> 00:40:52,440
+Autoclave اللي هو الجهاز اللي سمناه طين زراب ضغط
+
+558
+00:40:52,440 --> 00:40:57,620
+مطورة على درجة 115 15 15 ما قلنا 121 15 15 هذا
+
+559
+00:40:57,620 --> 00:41:02,300
+اللي بالظبط إذا هناك طرق متنوعة مختلفة من
+
+560
+00:41:05,130 --> 00:41:08,030
+هي أمريكا universal واحدة بت .. يعني بتسند عنهم
+
+561
+00:41:08,030 --> 00:41:12,590
+كلهم لأ كلهم موجودين كلهم موجودين فبحكيلكوا حاجة
+
+562
+00:41:12,590 --> 00:41:17,410
+من التاريخ اللي احنا احضرناها زماننا القبر و
+
+563
+00:41:17,410 --> 00:41:23,450
+السترنجات كانت reusable كانت زجاج .. ال .. ال ..
+
+564
+00:41:23,450 --> 00:41:28,150
+السترنجات زجاج و ال needles كانت حديد و الحديد هذا
+
+565
+00:41:28,150 --> 00:41:32,070
+كان له مسن عشان لما تحفة .. تحفة يعني الحد تبقى
+
+566
+00:41:32,070 --> 00:41:37,670
+تروح يسنوها عليهفكانت تغلى بالماء، وهذا كان
+
+567
+00:41:37,670 --> 00:41:41,010
+الإجراء اللي موجود، لا كان فيه autoclave ولا غيره،
+
+568
+00:41:41,010 --> 00:41:45,110
+تغلى في الماء، لكن كان يضعف بيكربونات ال sodium،
+
+569
+00:41:45,110 --> 00:41:49,290
+sodium بيكربونات، بيكربونات الصوية العفوية، عشان
+
+570
+00:41:49,290 --> 00:41:53,510
+تتقلل من درجة غلالة الماء، فترتفع الحرارة شوية،
+
+571
+00:41:53,510 --> 00:41:56,570
+وتزيد من قدرة ال penetration، فكانت يعني تحطمة
+
+572
+00:41:56,570 --> 00:42:01,170
+أفضر من الماء لحاله، فماء زاد sodium bicarbonate
+
+573
+00:42:03,620 --> 00:42:13,020
+خد بالكوا صحيح كان زجاج و حديد ال needle لكن
+
+574
+00:42:13,020 --> 00:42:17,940
+ماكانش فيه نفايات تخرج من ورا هذا الاستخدام بس
+
+575
+00:42:17,940 --> 00:42:20,680
+اليوم انتوا فى المعمل بس تتبرعوا تدري بواحد على
+
+576
+00:42:20,680 --> 00:42:24,240
+ساحة دم بيطلع معاكوا سلة مليانة مظبوطة اللي هي
+
+577
+00:42:24,240 --> 00:42:27,920
+plastics و sharps اللي هم ال needles و قد تحتوي
+
+578
+00:42:27,920 --> 00:42:33,120
+على مواد فيها hepatitis ب eggs whatsoever مظبوط؟
+
+579
+00:42:34,260 --> 00:42:38,360
+تكلفة التخلص من 1 كيلو جرام من النفايات الطبية
+
+580
+00:42:38,360 --> 00:42:44,200
+تعادل تكلفة 10 طن من النفايات المنزلية لذلك هناك
+
+581
+00:42:44,200 --> 00:42:47,840
+تفكير في بعض البلدان إلى العودة إلى ال reusable
+
+582
+00:42:47,840 --> 00:42:50,700
+العودة إلى ال reusable .. طبعا مع تقدم التقنيات
+
+583
+00:42:50,700 --> 00:42:55,640
+التعقيم الآن أصبح أكتر .. أكتر أمنا فلنستغرب فيهم
+
+584
+00:42:55,640 --> 00:42:58,940
+الأيام لو رجعنا ل .. اللي هو استخدام ال reusable
+
+585
+00:42:58,940 --> 00:43:05,370
+احنا أيامنا ال petri dishes كلها كانت pyrexكنا
+
+586
+00:43:05,370 --> 00:43:09,230
+نستخدم ال Petri dishes مرارا و تكرارا لغاية ما
+
+587
+00:43:09,230 --> 00:43:14,490
+ينكسر يعني زي أي إزوليات موجودة في المختبر لكن هذا
+
+588
+00:43:14,490 --> 00:43:18,270
+كان يقلل من إيه؟ من حرب النيفيات و طبعا حرب
+
+589
+00:43:18,270 --> 00:43:24,030
+المخاطر المصاحبة إليها إذا بدنا نفهم إن ال heat من
+
+590
+00:43:24,030 --> 00:43:28,070
+أحد أهم عناصر التعقيم اللي إحنا بنتعاملها أو
+
+591
+00:43:28,070 --> 00:43:31,850
+بنتعامل معها في our daily life في ال clinical و ال
+
+592
+00:43:31,850 --> 00:43:36,120
+medical microbiomeزي ما ذكرنا boiling,
+
+593
+00:43:36,340 --> 00:43:38,840
+autoclaving, dry heat انا لما احكي عن ال dry heat
+
+594
+00:43:38,840 --> 00:43:43,680
+صحيح ده ال dry heat الجافة بتاع في ال oven .. ال
+
+595
+00:43:43,680 --> 00:43:49,580
+oven بنسخن فيه لسخين حرارة جافة .. حرارة جافة درجة
+
+596
+00:43:49,580 --> 00:43:51,840
+الحرارة حكينا .. اعتقد حكيتها المرة الماضية اظنني
+
+597
+00:43:51,840 --> 00:43:57,340
+مية و سبعين درجة مقاوية لمدة ساعتين بينما ال
+
+598
+00:43:57,340 --> 00:44:00,620
+autoclaving كان كدهش؟ مية واحد و عشرين لمدة خمسة
+
+599
+00:44:00,620 --> 00:44:02,340
+عشر .. لمدة خمسة عشر
+
+600
+00:44:04,950 --> 00:44:09,590
+الوتوكلفة من أقل استهلاك للطاقة، إنه زمن، بتتكلم
+
+601
+00:44:09,590 --> 00:44:12,550
+عن مين أنا؟ بتتكلم عن fifteen minutes و بتتكلم عن
+
+602
+00:44:12,550 --> 00:44:19,730
+two hours لماذا الدرجتين مختلفتان؟ قولنا إن ال wet
+
+603
+00:44:19,730 --> 00:44:25,030
+heat ال penetration power تبعتها أعلى بكتير من ال
+
+604
+00:44:25,030 --> 00:44:29,580
+bright heatيعني بخار الماء عنده قدرة ال
+
+605
+00:44:29,580 --> 00:44:32,560
+penetration يعني انا افرض انه عندي انا استوانة ال
+
+606
+00:44:32,560 --> 00:44:36,020
+center تبعها هنا بلا تسخين من هنا wet heat و من
+
+607
+00:44:36,020 --> 00:44:39,940
+هنا dry heat سرعة وصول الحرارة الى ال center من
+
+608
+00:44:39,940 --> 00:44:43,700
+الجانب اللي فيه ال wet heat اعلى من سرعة وصول ال
+
+609
+00:44:43,700 --> 00:44:46,160
+dry heat الى .. يعني قدرة ال penetration بتكون
+
+610
+00:44:46,160 --> 00:44:48,060
+يعني قدرة اعلى كمان اخرى
+
+611
+00:44:57,230 --> 00:45:01,710
+بروح بيتقوع و بيغير من الأرقام هذه باختصار الوقت
+
+612
+00:45:01,710 --> 00:45:06,130
+او باختصار الحرارة فهو وقع الأمر هو بيغش نفسه و
+
+613
+00:45:06,130 --> 00:45:10,110
+بيغش الآخرين وهذه للأسف يعني ملاحظة لاحظناها عن
+
+614
+00:45:10,110 --> 00:45:14,350
+كثير من طباء الأسنان لما سألناهم جدش للدرجة
+
+615
+00:45:14,350 --> 00:45:18,470
+الحرارة فصار كل واحد يختلع رقم عنده 150 لمدة نص
+
+616
+00:45:18,470 --> 00:45:21,650
+ساعة بلا جودة 150 حرارة عالية كتير يعني فكيف يعني
+
+617
+00:45:21,650 --> 00:45:24,990
+لكن علميا قلنا لكم 170 for two hours
+
+618
+00:45:38,140 --> 00:45:40,560
+which hold protein chains together in their
+
+619
+00:45:40,560 --> 00:45:42,580
+secondary and tertiary structures يعني ال hydrogen
+
+620
+00:45:42,580 --> 00:45:46,200
+bonds لما اتفسق عن بعضها لبروتين ال structure كلها
+
+621
+00:45:46,200 --> 00:45:53,840
+تبقى بيصيرله ايه برضه denaturation حكيها
+
+622
+00:45:53,840 --> 00:45:56,260
+انا امكن لو .. هاي ال .. هاي ال .. الرقم اللي
+
+623
+00:45:56,260 --> 00:46:01,230
+ذكرناه احنا اللي هوالخمستاشر عشرين دقيقة مية واحدة
+
+624
+00:46:01,230 --> 00:46:05,410
+و عشرين درجة مئوية اللي هو ال fifteen pound per
+
+625
+00:46:05,410 --> 00:46:08,550
+square inch اللي حكينا عنها ال BSI اتصرت يعني الها
+
+626
+00:46:08,550 --> 00:46:17,470
+اه الهن فين مصطلح اسمه commercial sterilization
+
+627
+00:46:17,470 --> 00:46:22,230
+بعيد عن الموضوع بس لازم نتطرق اليه
+
+628
+00:46:33,000 --> 00:46:37,800
+فهي من ناحية صحية .. من ناحية صحية مؤقمة لكن من
+
+629
+00:46:37,800 --> 00:46:43,380
+ناحية ال organoleptics اللي قالوا الحواسي اللي ..
+
+630
+00:46:43,380 --> 00:46:48,820
+هتكون ايه غير صالحة فاقعاد اللي هم الصناع الأغضية
+
+631
+00:46:48,820 --> 00:46:52,180
+على ان بعض الأغضية لو احنا عملناها sterilization
+
+632
+00:46:52,180 --> 00:46:56,620
+كامل هتفقد أول حاجة بعض ال nutrients لما في بعض ال
+
+633
+00:46:56,620 --> 00:46:59,880
+nutrients بتكون ايه فرحوا اخترعوا مصطلح اسمه
+
+634
+00:46:59,880 --> 00:47:06,290
+commercial sterilizationتعقيم للتجارة، مش للطب،
+
+635
+00:47:06,290 --> 00:47:11,950
+للتجارة مش للطب، اشترط في حاجة واحدة، ان الغذاء
+
+636
+00:47:11,950 --> 00:47:17,670
+يكون خالي من جراتيم، اللي هي ال sports، الميكروبات
+
+637
+00:47:17,670 --> 00:47:22,670
+الممرضة، الميكروبات الممرضة، مااشترطوش ال absolute
+
+638
+00:47:22,670 --> 00:47:28,360
+killing of all forms of lifeاشتغلوا تخلوه المنتج
+
+639
+00:47:28,360 --> 00:47:32,320
+من ايه؟ لبطاري لجوا طرق انه يخفوا درجتها شويه عشان
+
+640
+00:47:32,320 --> 00:47:37,940
+يحافظوا على المنتج لكن طيب
+
+641
+00:47:37,940 --> 00:47:40,340
+احنا بيقولوا انه في بكتيريا Semiclostridium
+
+642
+00:47:40,340 --> 00:47:43,540
+Botulinum شرحنا عنها سابق عن ايه اتذكروا اللي
+
+643
+00:47:43,540 --> 00:47:46,000
+بياخدوا منها ال Botox اللي بياخدوا منها ال Botox
+
+644
+00:47:46,000 --> 00:47:51,880
+وذكرنا انه البكتيريا هذه Spore forming bacteria من
+
+645
+00:47:51,880 --> 00:47:56,320
+ال genus اللي هي Semiclostridiumو بتتحمل درجة
+
+646
+00:47:56,320 --> 00:48:01,680
+حرارة عالية ال auto cladding بالأرقام اللي ذكرناها
+
+647
+00:48:01,680 --> 00:48:05,660
+بيقتل هذا ال sports ال commercial sterilization
+
+648
+00:48:05,660 --> 00:48:12,680
+التعريف يعني حور حتى يضمن عدم وجود هذا ال micro في
+
+649
+00:48:12,680 --> 00:48:16,120
+الغدا يعني اشتراك ما اشتراك انه مايكونش فيها ال
+
+650
+00:48:16,120 --> 00:48:18,940
+pathogenic sports اللي من ضمنها مين ال clostridium
+
+651
+00:48:23,580 --> 00:48:27,140
+طيب احنا قولنا انه اذا كانت رقم 170 صحيح انا
+
+652
+00:48:27,140 --> 00:48:30,000
+بقوليها لو احنا قدامنا 100 .. اه مش موجود احنا
+
+653
+00:48:30,000 --> 00:48:32,920
+موجود احنا موجود احنا موجود احنا موجود احنا موجود
+
+654
+00:48:32,920 --> 00:48:32,920
+احنا موجود احنا موجود احنا موجود احنا موجود احنا
+
+655
+00:48:32,920 --> 00:48:32,920
+موجود احنا موجود احنا موجود احنا موجود احنا موجود
+
+656
+00:48:32,920 --> 00:48:32,940
+احنا موجود احنا موجود احنا موجود احنا موجود احنا
+
+657
+00:48:32,940 --> 00:48:33,760
+موجود احنا موجود احنا موجود احنا موجود احنا موجود
+
+658
+00:48:33,760 --> 00:48:33,800
+احنا موجود احنا موجود احنا موجود احنا موجود احنا
+
+659
+00:48:33,800 --> 00:48:39,200
+موجود احنا موجود
+
+660
+00:48:39,200 --> 00:48:43,400
+احنا
+
+661
+00:48:43,400 --> 00:48:48,460
+موج
+
+662
+00:48:51,290 --> 00:48:57,030
+حرارة 62 درجة مئوية في الحليب، الزمن اللازم
+
+663
+00:48:57,030 --> 00:49:04,210
+للبسترة كان نصف ساعة، تلاتين دقيقة على جداش ع 62
+
+664
+00:49:04,210 --> 00:49:09,290
+درجة مئوية، لما زدنا بناءت لدرجة الحرارة عشر درجات
+
+665
+00:49:09,290 --> 00:49:13,370
+مئوية سالة تلاتين وسبعين، نزل الزمن من تلاتين
+
+666
+00:49:13,370 --> 00:49:20,290
+دقيقة إلى خمستاشر ثانيةالخمس عشر ثانية وهذه يسموها
+
+667
+00:49:20,290 --> 00:49:25,530
+flash، flash يعني حد سريع، flash طبعا فيه أكثر
+
+668
+00:49:25,530 --> 00:49:32,030
+حرارة أعلى، بتاع في ال orange العصير المحلي تبعنا
+
+669
+00:49:32,030 --> 00:49:39,550
+غالبا فيه طعن لذا نوعا ماجانبًا هذا اللادع أو
+
+670
+00:49:39,550 --> 00:49:43,830
+الفعم اللادع جانبًا عملية اللي هو التعقيم أو اللي
+
+671
+00:49:43,830 --> 00:49:48,030
+هي البعفوة مش تعقيم بستوريزيش و بيسموها ultra high
+
+672
+00:49:48,030 --> 00:49:51,930
+treatment ultra يعني زي subra أو super أعلى من
+
+673
+00:49:51,930 --> 00:49:57,820
+subra حتى ultra ultra high treatmentبتعرض المنتج
+
+674
+00:49:57,820 --> 00:50:02,940
+لمدة one second لدرجة حرارة فجر المية، يعني لمية و
+
+675
+00:50:02,940 --> 00:50:06,340
+خمسة، ممكن مية و خمسين درجة قوية، for one second،
+
+676
+00:50:06,340 --> 00:50:08,160
+for one second، سموها ultra-hot
+
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/odJSCUX8GZQ_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/odJSCUX8GZQ_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..47ae50549b73ff5478e3fd53ecd0dc233819989c
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/odJSCUX8GZQ_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 4953, "start": 21.89, "end": 49.53, "text": "بسم الله الرحمن الرحيم احنا اتواصفنا المحاضرة الماضية عن تركيب من تركيب الخلية الداخلية اللي هو ال mesosome و بدنا نتعرف على مهيط ال mesosome هو عبارة عن invaginations نقول عنها هي بغى من مين؟ من ال cell، من ال brain، و ال cell و ال brain فيه وظائف عديدة، ماعرفش اتركني لها لسه، لكن هذه الوظائف", "tokens": [3555, 38251, 21984, 34892, 5016, 27842, 34892, 5016, 32640, 1975, 5016, 8315, 1975, 2655, 2407, 33546, 5172, 8315, 9673, 5016, 46958, 25720, 9673, 46958, 10632, 18871, 6055, 31747, 1829, 3555, 9154, 6055, 31747, 1829, 3555, 33962, 1211, 10632, 32748, 47283, 1211, 10632, 13672, 1829, 31439, 2423, 3813, 329, 423, 4032, 47525, 8315, 8717, 2655, 3615, 28480, 15844, 3714, 3224, 1829, 9566, 2423, 3813, 329, 423, 31439, 6225, 3555, 9640, 3660, 18871, 1048, 559, 10325, 8717, 39648, 18871, 11296, 39896, 4724, 17082, 7578, 9154, 3714, 9957, 22807, 9154, 2423, 2815, 12399, 9154, 2423, 3567, 12399, 4032, 2423, 2815, 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" glycocalyx", "probability": 0.86357421875}, {"start": 1044.69, "end": 1044.87, "word": " إلى", "probability": 0.36328125}, {"start": 1044.87, "end": 1045.17, "word": " أربعة", "probability": 0.7413736979166666}, {"start": 1045.17, "end": 1045.53, "word": " أصلا،", "probability": 0.574951171875}, {"start": 1045.53, "end": 1046.03, "word": " خلينا", "probability": 0.8143310546875}, {"start": 1046.03, "end": 1048.05, "word": " نكتبهم", "probability": 0.9380859375}, {"start": 1048.05, "end": 1048.65, "word": " على", "probability": 0.84912109375}, {"start": 1048.65, "end": 1049.31, "word": " الصرف،", "probability": 0.5985514322916666}, {"start": 1049.31, "end": 1049.89, "word": " glyco", "probability": 0.6116943359375}], "temperature": 1.0}, {"id": 39, "seek": 108300, "start": 1055.68, "end": 1083.0, "text": "الجليجوكاليكس قد يكون واحد من أربعة capsule micro capsule slime layer و slime على لزج يعني طبقة لزجة و هي كله أصلا لزجة هنشوف كمان شوية الوضعات تبعتها و S layer", "tokens": [6027, 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{"start": 1060.96, "end": 1061.38, "word": " أربعة", "probability": 0.73681640625}, {"start": 1061.38, "end": 1062.34, "word": " capsule", "probability": 0.53076171875}, {"start": 1062.34, "end": 1065.62, "word": " micro", "probability": 0.66455078125}, {"start": 1065.62, "end": 1066.32, "word": " capsule", "probability": 0.5703125}, {"start": 1066.32, "end": 1071.42, "word": " slime", "probability": 0.54345703125}, {"start": 1071.42, "end": 1071.92, "word": " layer", "probability": 0.939453125}, {"start": 1071.92, "end": 1075.46, "word": " و", "probability": 0.61572265625}, {"start": 1075.46, "end": 1075.66, "word": " slime", "probability": 0.55322265625}, {"start": 1075.66, "end": 1075.88, "word": " على", "probability": 0.364990234375}, {"start": 1075.88, "end": 1076.3, "word": " لزج", "probability": 0.7679036458333334}, {"start": 1076.3, "end": 1076.5, "word": " يعني", "probability": 0.847900390625}, {"start": 1076.5, "end": 1076.82, "word": " طبقة", "probability": 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9983, 49374, 15042, 2423, 20650, 4583, 5296, 3615, 15040, 28671, 13672, 1829, 49374, 16095, 16472, 3224, 6022, 9983, 6156, 3555, 28259, 23758, 25124, 1829, 38207, 2423, 2689, 1975, 1829, 8592, 2423, 47283, 2655, 15040, 5172, 22807], "avg_logprob": -0.2732928196589152, "compression_ratio": 1.9806763285024154, "no_speech_prob": 0.0, "words": [{"start": 1086.04, "end": 1086.64, "word": "الان", "probability": 0.8232421875}, {"start": 1086.64, "end": 1087.34, "word": " مال", "probability": 0.7091064453125}, {"start": 1087.34, "end": 1088.42, "word": " اتشابه", "probability": 0.835791015625}, {"start": 1088.42, "end": 1089.28, "word": " ومال", "probability": 0.733154296875}, {"start": 1089.28, "end": 1090.0, "word": " اختلاف", "probability": 0.9613037109375}, {"start": 1090.0, "end": 1090.4, "word": " بين", "probability": 0.9619140625}, {"start": 1090.4, "end": 1090.84, "word": " الأربع", "probability": 0.6747233072916666}, {"start": 1090.84, "end": 1091.18, "word": " هدول", "probability": 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0.73681640625}, {"start": 1096.46, "end": 1097.36, "word": " علاقة", "probability": 0.873046875}, {"start": 1097.36, "end": 1098.22, "word": " لعلاقة", "probability": 0.609130859375}, {"start": 1098.22, "end": 1098.36, "word": " اللي", "probability": 0.892822265625}, {"start": 1098.36, "end": 1098.68, "word": " بينهم", "probability": 0.9462890625}, {"start": 1098.68, "end": 1098.92, "word": " هدول", "probability": 0.9207356770833334}, {"start": 1098.92, "end": 1099.18, "word": " لإنه", "probability": 0.574310302734375}, {"start": 1099.18, "end": 1099.48, "word": " تنتين", "probability": 0.8684895833333334}, {"start": 1099.48, "end": 1100.1, "word": " organized", "probability": 0.759765625}, {"start": 1100.1, "end": 1104.32, "word": " بينما", "probability": 0.84716796875}, {"start": 1104.32, "end": 1104.44, "word": " ال", "probability": 0.64892578125}, {"start": 1104.44, "end": 1104.88, "word": " slime", "probability": 0.607421875}, {"start": 1104.88, "end": 1105.32, "word": " layer", "probability": 0.94287109375}, {"start": 1105.32, "end": 1105.7, "word": " لعلاقة", "probability": 0.81793212890625}, {"start": 1105.7, "end": 1105.84, "word": " اللي", "probability": 0.985595703125}, {"start": 1105.84, "end": 1106.08, "word": " بينهم", "probability": 0.941162109375}, {"start": 1106.08, "end": 1106.3, "word": " انه", "probability": 0.648193359375}, {"start": 1106.3, "end": 1106.68, "word": " none", "probability": 0.6923828125}, {"start": 1106.68, "end": 1109.36, "word": " organized", "probability": 0.89111328125}, {"start": 1109.36, "end": 1112.28, "word": " فبدا", "probability": 0.4842122395833333}, {"start": 1112.28, "end": 1112.5, "word": " هذا", "probability": 0.79541015625}, {"start": 1112.5, "end": 1112.78, "word": " الشيء", "probability": 0.7625325520833334}, {"start": 1112.78, "end": 1112.88, "word": " ال", "probability": 0.90869140625}, {"start": 1112.88, "end": 1113.12, "word": " common", "probability": 0.9892578125}, {"start": 1113.12, "end": 1113.36, "word": " ايش", "probability": 0.7279459635416666}, {"start": 1113.36, "end": 1114.08, "word": " الاختلاف؟", "probability": 0.9303385416666666}], "temperature": 1.0}, {"id": 41, "seek": 114315, "start": 1115.45, "end": 1143.15, "text": "الـ Thickness يعني الكابسول organized والمايكرو كابسول organized واضح أن كلمة كابسول هي الأضخم فال thickness يبقى عالي المايكرو كابسول organized بس ايه ال thickness صغير ال slime layer مش organized لكن thick ال S layer مش organized لكن thin", "tokens": [6027, 39184, 334, 618, 1287, 37495, 22653, 33251, 16758, 3794, 12610, 9983, 16070, 15042, 1829, 4117, 32887, 9122, 16758, 3794, 12610, 9983, 4032, 46958, 5016, 14739, 9122, 19528, 3660, 9122, 16758, 3794, 12610, 39896, 16247, 11242, 9778, 2304, 6156, 6027, 14855, 7251, 3555, 4587, 7578, 6225, 6027, 1829, 9673, 47302, 4117, 32887, 9122, 16758, 3794, 12610, 9983, 4724, 3794, 1975, 1829, 3224, 2423, 14855, 20328, 17082, 13546, 2423, 20650, 4583, 37893, 9983, 44381, 5060, 2423, 318, 4583, 37893, 9983, 44381, 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1129.31, "word": " كلمة", "probability": 0.9181315104166666}, {"start": 1129.31, "end": 1129.85, "word": " كابسول", "probability": 0.83953857421875}, {"start": 1129.85, "end": 1130.09, "word": " هي", "probability": 0.81591796875}, {"start": 1130.09, "end": 1130.71, "word": " الأضخم", "probability": 0.85693359375}, {"start": 1130.71, "end": 1131.27, "word": " فال", "probability": 0.946044921875}, {"start": 1131.27, "end": 1131.49, "word": " thickness", "probability": 0.90673828125}, {"start": 1131.49, "end": 1131.77, "word": " يبقى", "probability": 0.80572509765625}, {"start": 1131.77, "end": 1132.33, "word": " عالي", "probability": 0.8095703125}, {"start": 1132.33, "end": 1133.01, "word": " المايكرو", "probability": 0.72332763671875}, {"start": 1133.01, "end": 1133.47, "word": " كابسول", "probability": 0.9913330078125}, {"start": 1133.47, "end": 1133.91, "word": " organized", "probability": 0.90185546875}, {"start": 1133.91, "end": 1134.15, "word": " بس", "probability": 0.9443359375}, {"start": 1134.15, "end": 1134.39, "word": " ايه", "probability": 0.7845052083333334}, {"start": 1134.39, "end": 1134.59, "word": " ال", "probability": 0.378173828125}, {"start": 1134.59, "end": 1134.83, "word": " thickness", "probability": 0.9619140625}, {"start": 1134.83, "end": 1135.39, "word": " صغير", "probability": 0.9827473958333334}, {"start": 1135.39, "end": 1135.83, "word": " ال", "probability": 0.6904296875}, {"start": 1135.83, "end": 1136.23, "word": " slime", "probability": 0.41455078125}, {"start": 1136.23, "end": 1136.59, "word": " layer", "probability": 0.9091796875}, {"start": 1136.59, "end": 1137.07, "word": " مش", "probability": 0.9765625}, {"start": 1137.07, "end": 1137.69, "word": " organized", "probability": 0.94677734375}, {"start": 1137.69, "end": 1138.49, "word": " لكن", "probability": 0.91259765625}, {"start": 1138.49, "end": 1139.27, "word": " thick", "probability": 0.568359375}, {"start": 1139.27, "end": 1140.05, "word": " ال", "probability": 0.9599609375}, {"start": 1140.05, "end": 1140.31, "word": " S", "probability": 0.427490234375}, {"start": 1140.31, "end": 1140.67, "word": " layer", "probability": 0.76904296875}, {"start": 1140.67, "end": 1140.99, "word": " مش", "probability": 0.98681640625}, {"start": 1140.99, "end": 1141.57, "word": " organized", "probability": 0.943359375}, {"start": 1141.57, "end": 1142.19, "word": " لكن", "probability": 0.96533203125}, {"start": 1142.19, "end": 1143.15, "word": " thin", "probability": 0.77099609375}], "temperature": 1.0}, {"id": 42, "seek": 116992, "start": 1143.48, "end": 1169.92, "text": "و هي الفروقات بين الأربعة، إذا لو سمعتوا في يوم من الأيام مصطلح glycocalyx، capsule و لا micro capsule و لا S layer و لا slam layer، كلهم في النهاية هم اللي أنتوا بتعرفوهم باسم مين؟ باسم capsule، باسم capsule، لكن معنين إنه نقلنه، كتير من الكتب صارت تتكلم عنهم بضرر مختلفة و متنوعة، فكويس إن أنتوا عارفين هذه المعلومة", "tokens": [2407, 39896, 27188, 2288, 30543, 9307, 49374, 16247, 25513, 27884, 12399, 11933, 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ببعض الأيونات و المونيكيولز these can then be made available to the cells هاي الخلية البكتيرية هاي ال slime layer تمام لأن بتاعنا في بعض الأحيان مينقدرش احنا نتابع", "tokens": [3522, 307, 257, 26316, 3714, 9778, 11622, 1863, 337, 1629, 17617, 5296, 3555, 3615, 11242, 14739, 14407, 3615, 9154, 9673, 14407, 3215, 9673, 17082, 8848, 10632, 2423, 39184, 22633, 66, 905, 5222, 87, 8032, 6027, 3224, 6055, 2407, 11242, 1829, 5016, 5296, 11296, 4724, 2407, 8315, 7251, 30544, 12174, 18513, 2288, 15844, 14739, 7251, 43500, 3555, 9566, 4724, 3555, 3615, 11242, 16247, 1829, 2407, 8315, 2655, 4032, 9673, 11536, 1829, 4117, 1829, 12610, 11622, 613, 393, 550, 312, 1027, 2435, 281, 264, 5438, 8032, 47302, 33962, 1211, 10632, 29739, 4117, 2655, 13546, 10632, 8032, 47302, 2423, 20650, 4583, 46811, 10943, 5296, 33456, 39894, 45761, 8315, 8978, 45030, 11242, 16247, 5016, 1829, 7649, 3714, 9957, 28543, 2288, 8592, 1975, 5016, 8315, 8717, 2655, 16758, 3615], "avg_logprob": -0.36975000953674314, 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إلى الأمام، طبعا برضه ه .. هعرضلكم المحاضرة الجاية ان شاء الله بعض الفلاشات، انها هتكون المحاضرة الجاية غالبا", "tokens": [6027, 5172, 1211, 26108, 37977, 6225, 3555, 9640, 3660, 18871, 4386, 16472, 2655, 6225, 9640, 5172, 9957, 22807, 11331, 26108, 3660, 3714, 2288, 46007, 12399, 20328, 35473, 12399, 46811, 10943, 22807, 6156, 6027, 148, 48506, 6055, 2655, 5016, 31747, 27188, 1211, 26108, 37977, 39627, 15730, 25124, 28820, 4032, 4117, 33456, 11296, 39894, 8592, 2655, 17082, 1211, 4724, 9566, 16572, 28671, 11778, 31845, 27884, 12399, 23032, 16572, 28671, 6225, 3555, 9640, 3660, 18871, 3714, 35473, 13063, 39894, 2655, 5016, 31747, 39627, 15730, 25124, 28820, 12399, 6156, 21292, 3215, 5172, 3615, 29739, 4117, 2655, 13546, 25528, 30731, 16247, 2304, 10943, 12399, 23032, 3555, 3615, 995, 4724, 43042, 3224, 8032, 4386, 8032, 3615, 43042, 23275, 2304, 9673, 5016, 46958, 25720, 25724, 995, 10632, 16472, 13412, 16606, 21984, 45030, 11242, 27188, 1211, 33599, 9307, 12399, 16472, 11296, 8032, 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\ No newline at end of file
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/siSk8ZzrN8Q.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/siSk8ZzrN8Q.srt
new file mode 100644
index 0000000000000000000000000000000000000000..4fdfa0959cf879da46debbd54c62882016fc8e2d
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/siSk8ZzrN8Q.srt
@@ -0,0 +1,1779 @@
+1
+00:00:20,540 --> 00:00:23,720
+بسم الله الرحمن الرحيم نكمل الـ biogenic kokai
+
+2
+00:00:23,720 --> 00:00:26,960
+المحاضرة السابقة تحدثتنا عن الـ streptococcus
+
+3
+00:00:26,960 --> 00:00:30,340
+biogens والـ streptococcus الجالكتيا وبدأنا 
+
+4
+00:00:30,340 --> 00:00:34,020
+بالحديث عن الـ group D streptococci أخذنا صفة
+
+5
+00:00:34,020 --> 00:00:37,920
+مشتركة صفة مشتركة أنه كل الـ group D streptococci
+
+6
+00:00:37,920 --> 00:00:42,480
+can grow on a medium known as bile-esculin agar و
+
+7
+00:00:42,480 --> 00:00:47,810
+قلنا أن الصفتين الـ growth in bile والـ hydrolysis of
+
+8
+00:00:47,810 --> 00:00:51,630
+esculin يجب أن يتوفروا في الميكروب حتى يستطيع أنه 
+
+9
+00:00:51,630 --> 00:00:54,350
+ينجح في هذا الاختبار أنه يكون positive قادر على
+
+10
+00:00:54,350 --> 00:00:57,790
+النمو في الـ bile ومن ثم قادر على استغلال اللي هو
+
+11
+00:00:57,790 --> 00:01:03,950
+الـ esculin إذا كانت الصفة موجودة فالبكتيريا group
+
+12
+00:01:03,950 --> 00:01:08,210
+D streptococci الآن كيف نميز enterococcus عن non
+
+13
+00:01:08,210 --> 00:01:11,510
+enterococcus بواسطة الـ growth أو الـ ability to
+
+14
+00:01:11,510 --> 00:01:16,170
+grow in 6.5% sodium chloride if positive it is
+
+15
+00:01:16,170 --> 00:01:20,790
+enterococcus if negative it is non-enterococcus
+
+16
+00:01:20,790 --> 00:01:25,470
+كلمة non-enterococcus ما حد سأل لماذا ندرس الآن
+
+17
+00:01:25,470 --> 00:01:28,390
+group D streptococci وبنتكلم عن مين؟ عن
+
+18
+00:01:28,390 --> 00:01:31,310
+enterococcus ما علاقة الـ enterococcus بالـ
+
+19
+00:01:31,310 --> 00:01:32,490
+streptococcus؟
+
+20
+00:01:34,400 --> 00:01:38,760
+الجواب في اللوحة هذه They are distantly related to
+
+21
+00:01:38,760 --> 00:01:42,440
+other streptococcus يمكن أن الـ enterococcus لها قرابة
+
+22
+00:01:42,440 --> 00:01:46,040
+بعيدة مع الـ streptococcus أيام ما درسنا احنا
+
+23
+00:01:46,040 --> 00:01:50,700
+microbiology كان اسمها streptococcus يعني كان...
+
+24
+00:01:50,700 --> 00:01:53,440
+كنا بنقول Streptococcus fecalis اليوم بنقول
+
+25
+00:01:53,440 --> 00:01:57,500
+Enterococcus faecalis يبقى كانت وصلنا في يوم من
+
+26
+00:01:57,500 --> 00:02:00,440
+الأيام ايه؟ Streptococcus لأنه بتمتلك بعض الصفات
+
+27
+00:02:00,440 --> 00:02:02,800
+الشبيهة فيها من ضمنها أنها catalase
+
+28
+00:02:02,800 --> 00:02:07,720
+negative كمان تشترك معاها في الـ
+
+29
+00:02:07,720 --> 00:02:09,860
+antigens عشان احنا قلنا group A و group B و group
+
+30
+00:02:09,860 --> 00:02:12,340
+...يعني معنا الحاجة أن هي تمتلك الـ antigens اللي
+
+31
+00:02:12,340 --> 00:02:17,310
+بتبقى Streptococcus لكن ...سميت entero لأنها موجودة
+
+32
+00:02:17,310 --> 00:02:20,310
+كـ normal flora of the intestinal tract أو بيسموها
+
+33
+00:02:20,310 --> 00:02:23,510
+gut flora gut flora يعني intestinal tract flora
+
+34
+00:02:23,510 --> 00:02:27,690
+كونها gut flora خذوا بالكم أذكركم بالإشريشيا
+
+35
+00:02:27,690 --> 00:02:31,650
+كولاية الإشريشيا كولاية gut flora ولا لأ؟ مقطوط و
+
+36
+00:02:31,650 --> 00:02:36,130
+بتسبب ايه؟ بتسبب urinary tract infection number
+
+37
+00:02:36,130 --> 00:02:38,810
+one cause of urinary tract infection كانت الإشريشيا
+
+38
+00:02:38,810 --> 00:02:46,140
+كولاية ليش؟ لأنها قريبة للجهاز البولي من اللي هو
+
+39
+00:02:46,140 --> 00:02:51,280
+فتحة الإخراج وهي كذلك أكثر عند النساء من عن من
+
+40
+00:02:51,280 --> 00:02:55,920
+اللي هو الذكور وبالتالي بتوقع أن الـ enterococcus
+
+41
+00:02:55,920 --> 00:03:00,380
+كمان بتسبب urinary tract infection ناتجة عن مين؟
+
+42
+00:03:00,380 --> 00:03:04,520
+عن fecal contamination كمان بتسبب opportunistic
+
+43
+00:03:04,520 --> 00:03:09,090
+infection وكلمة opportunistic ده يجب أن نتذكر أنه
+
+44
+00:03:09,090 --> 00:03:13,730
+بتصيب الناس اللي المناعة تبعتهم مُنخافرة انخفاض
+
+45
+00:03:13,730 --> 00:03:16,050
+المناعة بيكون غالبا وين؟ في أي مكان، في
+
+46
+00:03:16,050 --> 00:03:18,850
+المستشفيات، المرضى اللي موجودين في المستشفيات
+
+47
+00:03:18,850 --> 00:03:23,530
+وبالتالي بتوقع أن الميكروب هذا ايه له يعني خدنا
+
+48
+00:03:23,530 --> 00:03:28,130
+نقول انتشار واسع في المستشفيات أحد كمان أسباب
+
+49
+00:03:28,130 --> 00:03:31,990
+انتشاره الواسع مقدرة للبكتيريا اللي اسمها
+
+50
+00:03:31,990 --> 00:03:36,570
+Enterococcus على تكوين الـ resistance من نجوم، مش
+
+51
+00:03:36,570 --> 00:03:39,290
+قلنا سنة في الـ coccus أولى سنة المرة الماضية، لسنة
+
+52
+00:03:39,290 --> 00:03:41,330
+الـ coccus البرجينز ما قلنا عنها، إنها من نجوم الـ
+
+53
+00:03:41,330 --> 00:03:44,830
+resistance، أما؟ ولا الأجلات كتير، لكن أنا بقول كل
+
+54
+00:03:44,830 --> 00:03:48,890
+يوم الـ enterococcus هي من نجوم الـ resistance،
+
+55
+00:03:48,890 --> 00:03:51,350
+المصطلح اللي أطلقته المرة الماضية على الـ vancomycin
+
+56
+00:03:51,350 --> 00:03:56,460
+resistant تعالوا نشوفه بس جاي نتطلع على الكلمة الأخيرة
+
+57
+00:03:56,460 --> 00:04:00,660
+في اللوحة Most Common Species Encountered in
+
+58
+00:04:00,660 --> 00:04:03,900
+Clinical Laboratory أو In Clinical Medicine أو In
+
+59
+00:04:03,900 --> 00:04:07,200
+Clinical Practice in Enterococcus faecalis اللي هي
+
+60
+00:04:07,200 --> 00:04:10,460
+أخت تانية اسمها Enterococcus faecium والتنتين
+
+61
+00:04:10,460 --> 00:04:13,620
+بدلوا على نفس الـ origin اللي هو الـ fecal material
+
+62
+00:04:13,620 --> 00:04:19,500
+يعني من الـ intestinal tract نتطلع على المصطلح اللي
+
+63
+00:04:19,500 --> 00:04:23,820
+أطلقناه المرة الماضية اللي هو الـ VRE Vancomycin
+
+64
+00:04:23,820 --> 00:04:29,180
+-resistant Enterococcus مش فيها أنها مصطلح تاني
+
+65
+00:04:29,180 --> 00:04:34,260
+ممتسلة resistant Staphylococcus aureus MRSA
+
+66
+00:04:34,260 --> 00:04:39,780
+قلنا من المصطلحات المهمة جدا في عصرنا الحالي و
+
+67
+00:04:39,780 --> 00:04:42,580
+ذكرتلكم معلومة وقلت أن الـ Vancomycin واعتبر من
+
+68
+00:04:42,580 --> 00:04:45,460
+قبل مانعضة نصحة العالمية أنه هو الـ ...هو الـ ...
+
+69
+00:04:45,460 --> 00:04:48,860
+الـ ...الـ ...الطلقة الأخيرة في ترسانة الطب ضد الـ
+
+70
+00:04:48,860 --> 00:04:51,780
+gram-positive cocci واحنا هنا جايين بنلاحظ أنه كان
+
+71
+00:04:51,780 --> 00:04:54,640
+Vancomycin resistant وأنا بقولكم في القطاع اللذة
+
+72
+00:04:54,640 --> 00:04:58,740
+الـ Vancomycin resistant في الـ Enterococcus عالية جدا...
+
+73
+00:04:58,740 --> 00:05:03,260
+قد تصل إلى 60%...إلى 60%...اه افرض...الـ...الـ
+
+74
+00:05:03,260 --> 00:05:07,240
+...الـ...عملت الـ resistance طبعا للي مش عارف
+
+75
+00:05:07,240 --> 00:05:10,100
+ميكانيزمه بـ action بالتأكيد مش هعرف اللي هو الـ
+
+76
+00:05:10,100 --> 00:05:13,740
+resistance لذا بدي أوضح أنا كيف بيشتغل الـ
+
+77
+00:05:13,740 --> 00:05:16,020
+vancomycin لكي نعرف كيف بيصير اللي هو الـ
+
+78
+00:05:16,020 --> 00:05:21,320
+resistance ركزوا معايا تكوين الـ cell wall لـ gram
+
+79
+00:05:21,320 --> 00:05:23,880
+positive كان فيه اندي multiple layers of
+
+80
+00:05:23,880 --> 00:05:28,900
+peptidoglycan صحيح؟ والـ multiple layers were
+
+81
+00:05:28,900 --> 00:05:33,980
+cross-linked وده short peptide cross-linked كلمة
+
+82
+00:05:33,980 --> 00:05:37,700
+cross-link وقلنالكم مهمة جدا بدون cross-link
+
+83
+00:05:37,700 --> 00:05:40,440
+الطبقات هتزلة collapse على بعضها والـ structure
+
+84
+00:05:40,440 --> 00:05:45,300
+بيكون ضعيف جدا فعندما لا يتكون الـ cross-link بيفشل
+
+85
+00:05:45,300 --> 00:05:49,860
+نظام السلوك الـ vancomycin بيشتغل على أنه بيرتبط
+
+86
+00:05:49,860 --> 00:05:55,660
+بيرتبط بـ D-alanine تمام؟ وبيمنع عملية تكون
+
+87
+00:05:55,660 --> 00:06:00,260
+اللي هو الـ cross-link البكتيريا هي موجودة على الله
+
+88
+00:06:00,620 --> 00:06:04,920
+الصبورة دي D-alanine بتغير وبت...البكتيريا بتحوله أو
+
+89
+00:06:04,920 --> 00:06:08,900
+...أو تماله replacement بـ D-lactate الآن الـ
+
+90
+00:06:08,900 --> 00:06:12,580
+receptor تبع الـ vancomycin بطل موجود إذا مافي
+
+91
+00:06:12,580 --> 00:06:16,140
+receptor له لن يستطيع أن يرتبط إذا لم يستطيع أن
+
+92
+00:06:16,140 --> 00:06:18,620
+يرتبط لن يقوم بوظيفته وبالتالي البكتيريا أصبحت
+
+93
+00:06:18,620 --> 00:06:26,960
+مقاومة لـ الـ vancomycin واضح أنه من الميكروبات الـ
+
+94
+00:06:26,960 --> 00:06:31,260
+...الـ...اللي تعلمت بسرعة ونقلت خبرتها لزملائها في
+
+95
+00:06:31,260 --> 00:06:34,300
+العالم اللي هي الـ enterococcus لذلك أصبح عندنا
+
+96
+00:06:34,300 --> 00:06:36,300
+مصطلح مشهور جدا اسمه vancomycin resistant
+
+97
+00:06:36,300 --> 00:06:41,300
+Enterococcus تخيلوا يا بنات أنه مافي بديل عن الـ
+
+98
+00:06:41,300 --> 00:06:44,760
+vancomycin هو في بدائل بس احنا كان قبل فترة لكن
+
+99
+00:06:44,760 --> 00:06:48,120
+تخيلوا أن مصر أنه مافي بديل أو أن البدائل limited
+
+100
+00:06:48,120 --> 00:06:52,600
+أو أن البدائل very expensive وده كلام صحيح وواحد
+
+101
+00:06:52,600 --> 00:06:55,620
+عنده urinary tract infection...simple urinary
+
+102
+00:06:55,620 --> 00:06:57,820
+tract infection اللي كنت انعالجها بأبسط الـ
+
+103
+00:06:57,820 --> 00:07:00,760
+antibiotic ومش جدين انعالجها...تتوقع ايش يصير...
+
+104
+00:07:00,760 --> 00:07:04,970
+ايش يصير في المرضى؟ الـ UTI هتصير الـ complication
+
+105
+00:07:04,970 --> 00:07:08,150
+هتعمل الـ pyelonephritis التهاب في الكلية نفسها و
+
+106
+00:07:08,150 --> 00:07:10,450
+قد تصل إلى الدم تعمل الـ septicemia وقد تصل إلى الـ
+
+107
+00:07:10,450 --> 00:07:13,290
+meningitis تعمل الـ...الـ meningus تعمل الـ
+
+108
+00:07:13,290 --> 00:07:18,050
+meningitis وبالتالي يموت المريض من infection بسيطة
+
+109
+00:07:18,050 --> 00:07:20,730
+كانت تعالج بسبب الـ antibiotic لعدم وجود
+
+110
+00:07:20,730 --> 00:07:26,350
+antibiotic في عالم ضده وهذا هو الـ worst scenario
+
+111
+00:07:26,350 --> 00:07:31,090
+المتوقع في العشرة أعوام القادمة لذلك بنلاقي أن كل
+
+112
+00:07:31,090 --> 00:07:34,570
+العالم ومن ضمن منظمات الصحة العالمية ومن ضمن
+
+113
+00:07:34,570 --> 00:07:38,430
+منظمات دولية حتى الحكومات صارت تتكلم عن هذا
+
+114
+00:07:38,430 --> 00:07:40,290
+الموضوع وعن الموضوع الـ antimicrobial resistance
+
+115
+00:07:40,290 --> 00:07:44,450
+أن لازم نجي له حل نبطئ الظاهرة نعالج الظاهرة نبحث
+
+116
+00:07:44,450 --> 00:07:49,310
+عن بدائل جديدة والبحث عن بداية جديدة جاري العمل
+
+117
+00:07:49,310 --> 00:07:52,450
+فيها، أنا هأقول لكم عن بديل، فاكرين؟ الـ...الـ...الـ
+
+118
+00:07:52,450 --> 00:07:56,970
+bacteriophage أنه نستخدم الـ bacteriophage في قتل الـ
+
+119
+00:07:56,970 --> 00:08:00,090
+...الـ...الـ...الـ bacteria نعم، يعمل علاج لـ
+
+120
+00:08:00,090 --> 00:08:03,290
+infection في كمان شغل تاني اسمها quorum sensing،
+
+121
+00:08:03,290 --> 00:08:06,390
+فاكرينها؟ أن البكتيريا بتتكلم مع بعضها وبتعرف أنش
+
+122
+00:08:06,390 --> 00:08:11,320
+هددها، لو أنا...عملت نوع من الـ...الـ...الـ...الـ
+
+123
+00:08:11,320 --> 00:08:15,740
+interference يعني خربت...شووشت...شووشت بمواد
+
+124
+00:08:15,740 --> 00:08:20,320
+كيميائية أخرى على البكتيريا مثلا كانوا عددها مية و
+
+125
+00:08:20,320 --> 00:08:23,600
+...وخلتها تفكر حالة أنها...أنها مليار حاسيت انتش 
+
+126
+00:08:23,600 --> 00:08:26,800
+إجلال الـ violence factor هتنبه جهاز المناعة و بتجزّئ
+
+127
+00:08:26,800 --> 00:08:31,090
+المناعة بيقضي عليها، لقايافيه هناك بدائية كثيرة،
+
+128
+00:08:31,090 --> 00:08:33,230
+البحث عن new antibiotics, new compounds,
+
+129
+00:08:33,650 --> 00:08:36,830
+modification of antibiotics اللي موجودة أساسًا، كلّ
+
+130
+00:08:36,830 --> 00:08:43,150
+هذه طرق قد تساهم، لكن واضح أنه we are losing the
+
+131
+00:08:43,150 --> 00:08:48,250
+battle، احنا بنخسر المعركة مع الـ micro لحد الآن،
+
+132
+00:08:48,250 --> 00:08:52,990
+هذا اللي بيبدو ..مجموعة أخرى غير الـ group D 
+
+133
+00:08:52,990 --> 00:08:56,970
+streptococci اسمها viridans streptococci الـ
+
+134
+00:08:56,970 --> 00:09:00,490
+viridans معناها the green streptococci وسمّت الـ
+
+135
+00:09:00,490 --> 00:09:03,610
+green لأنها alpha hemolytic يعني على الطبق على بطء
+
+136
+00:09:03,610 --> 00:09:07,090
+جهرش اللي كلّه منها أو المستعمرات حواليها هتكون
+
+137
+00:09:07,090 --> 00:09:13,250
+خضرة diverse species متنوعة، قرّال في السكن الـ
+
+138
+00:09:13,250 --> 00:09:14,990
+habitat تبعتها في الفن
+
+139
+00:09:19,200 --> 00:09:22,180
+الأمراض اللي بتسببها it is alpha hemolytic and
+
+140
+00:09:22,180 --> 00:09:26,100
+negative for other tests ما أعرفش يعني هذه جملة
+
+141
+00:09:26,100 --> 00:09:29,520
+غامضة لو واحد يعني بدّي أقرأها لأول مرة بالفعل هي
+
+142
+00:09:29,520 --> 00:09:34,280
+غامضة Alpha hemolytic ما في يعني خلاف and negative
+
+143
+00:09:34,280 --> 00:09:38,780
+for other tests negative for other tests that are
+
+144
+00:09:38,780 --> 00:09:41,240
+performed for the similar alpha hemolytic
+
+145
+00:09:41,240 --> 00:09:47,690
+streptococcus pneumonia لما احنا بندور على صفات
+
+146
+00:09:47,690 --> 00:09:51,610
+تمييزية، بندور عليها لمين؟ لاثنين مختلفين ولا
+
+147
+00:09:51,610 --> 00:09:56,630
+لاثنين متشابهين؟ لاثنين متشابهين مش قادرين
+
+148
+00:09:56,630 --> 00:10:00,390
+يتميّزوا مع بعض، طب نحاول ندور على صفات، تفاصيل،
+
+149
+00:10:00,390 --> 00:10:02,310
+عشان يتميّزوا مع بعض، ليش ده الحصانين الأبيض
+
+150
+00:10:02,310 --> 00:10:07,200
+والأسود؟ ما فيش ده عنهم مظبوط تمييز بينهم، فإحنا عندي
+
+151
+00:10:07,200 --> 00:10:10,520
+الـ Stripped Caucas pneumonia و Stripped Caucas
+
+152
+00:10:10,520 --> 00:10:14,340
+viridance بشكل عام بيشبهوا بعض بينهم الاثنين ألفة
+
+153
+00:10:14,340 --> 00:10:18,080
+هيموريتك الاثنين دبلو كوكاية الاثنين دبلو كوكاية فكيف
+
+154
+00:10:18,080 --> 00:10:22,260
+نميزهم عن بعض؟ في few tests positive للـ Stripped
+
+155
+00:10:22,260 --> 00:10:26,280
+Caucas pneumonia كلهم و negative لمن؟ للـ viridance
+
+156
+00:10:26,280 --> 00:10:29,400
+group فلما نتعلم الـ test تبعه Stripped Caucas
+
+157
+00:10:29,400 --> 00:10:34,740
+pneumonia هنفهم الجملة هذه وإنه هي negative it is
+
+158
+00:10:34,740 --> 00:10:37,860
+non-groupable والـ non-grover برضه صفة تشترك فيها
+
+159
+00:10:37,860 --> 00:10:41,260
+مع مين؟ مع الـ streptococcus pneumonia صح؟ الاثنين
+
+160
+00:10:41,260 --> 00:10:45,780
+non-grover it includes streptococcus mutants اللي
+
+161
+00:10:45,780 --> 00:10:51,060
+ممكن تسبب endocarditis خلال عملية خلع الأسنان أو
+
+162
+00:10:51,060 --> 00:10:56,260
+الطحون لما واحد بيخلع سن أو طحونة برضه عملية عنيفة
+
+163
+00:10:56,260 --> 00:11:00,780
+يا بنات وفيها تمزيق للأنسجة و بينزل دم بكميات
+
+164
+00:11:00,780 --> 00:11:05,100
+كبيرة مش زي أسنان الحليب واللبن تمام؟
+
+165
+00:11:07,430 --> 00:11:11,150
+فبالتالي الفم كلكم عارفين هو بيحتوي على عدد كبير
+
+166
+00:11:11,150 --> 00:11:14,930
+من الميكروبينزين وهذه البكتيريا oral إمكانية أنه
+
+167
+00:11:14,930 --> 00:11:21,170
+يصل ميكروبات إلى الدم عالية جدا وبالتالي الأطباء
+
+168
+00:11:21,170 --> 00:11:25,370
+الأسنان بيعطونا antibiotics البعض ما بيلتزم أما لا
+
+169
+00:11:25,370 --> 00:11:28,350
+بيلتزمش بالجرعة أو بيلتزمش بالـ course نفسه أو
+
+170
+00:11:28,350 --> 00:11:33,440
+ما بياخدش من أساسه antibiotics فقد تنتقل الـ
+
+171
+00:11:33,440 --> 00:11:37,060
+streptococcus mutants من الفم إلى الدم إلى القلب
+
+172
+00:11:37,060 --> 00:11:40,480
+وتسبب إنه ما يسمى بالـ endocarditis الـ .. الـ ..
+
+173
+00:11:40,480 --> 00:11:45,920
+العصبورة شرحناه ولا شرحناوش هذا؟ ASOT Anti
+
+174
+00:11:45,920 --> 00:11:48,160
+Streptolysin O أذكركوا فيه بس على السريع
+
+175
+00:11:48,160 --> 00:11:53,800
+Streptolysin O S O S O Streptolysin O بما أنه هو
+
+176
+00:11:53,800 --> 00:11:59,510
+الهيموليسين اللي في الـ streptococcus و بيحلل الدم،
+
+177
+00:11:59,510 --> 00:12:03,370
+لكن أحد مزاياه أنه لو اتعرض للأكسجين بتدمر، سمّيناه
+
+178
+00:12:03,370 --> 00:12:07,530
+Oxidin Lapide Streptolysin O الـ Antibody اللي
+
+179
+00:12:07,530 --> 00:12:12,430
+بيطلعله سمّيناه Antistreptolysin O ASO
+
+180
+00:12:13,930 --> 00:12:17,290
+لما اتفقنا إن معظمنا أصيب في يوم من الأيام ..
+
+181
+00:12:17,290 --> 00:12:20,030
+أصيب بماذا؟ بالـ tonsillitis، يعني بـ
+
+182
+00:12:20,030 --> 00:12:22,530
+streptococcus pyidins، يعني في عندنا antibodies
+
+183
+00:12:22,530 --> 00:12:26,830
+معظمنا لهذه البكتيريا، سواء كنا الآن مرضى أو
+
+184
+00:12:26,830 --> 00:12:30,770
+ما كنا مرضى، فلو عملنا فحص، للكلّ حلّا يجيب
+
+185
+00:12:30,770 --> 00:12:33,090
+positive، فكيف نبيّز بينه .. بين recent
+
+186
+00:12:33,090 --> 00:12:35,650
+infection و بين previous infection فعملنا
+
+187
+00:12:35,650 --> 00:12:39,520
+الـ titer، الـ titer معناها نقياس، مقياس تركيز الـ
+
+188
+00:12:39,520 --> 00:12:43,520
+antibodies في الجسم إذا كان فوق المعدل فهو recent
+
+189
+00:12:43,520 --> 00:12:48,220
+إذا أقل من المعدل فهو negative فحص Antigen
+
+190
+00:12:48,220 --> 00:12:53,110
+Antibody reaction إيش بنسميه؟ بنجوسان سيرولوجي، يبقى
+
+191
+00:12:53,110 --> 00:12:57,150
+فحوصات الـ serology هي عبارة عن فحوصات معتمدة على
+
+192
+00:12:57,150 --> 00:13:00,250
+.. في الـ reaction تبعها على ثلاثة عناصر، إيش عبارة
+
+193
+00:13:00,250 --> 00:13:03,530
+عن ثلاثة عناصر؟ الـ antigen، الـ antibody و الـ
+
+194
+00:13:03,530 --> 00:13:06,590
+complement، فدول الثلاثة تقريبًا هم الـ .. طبعًا في
+
+195
+00:13:06,590 --> 00:13:09,510
+.. في أشكال وألوان في الـ .. في الـ serology، لكن
+
+196
+00:13:09,510 --> 00:13:12,530
+في الآخر هي العناصر الرئيسية، antigen, antibody
+
+197
+00:13:12,530 --> 00:13:16,770
+and complement إحنا كنا إحنا طلاب نقول عنه علم
+
+198
+00:13:16,770 --> 00:13:21,080
+المنبتولوجي، ليش علم المنبتولوجي؟ لأننا بنحط نقطة
+
+199
+00:13:21,080 --> 00:13:25,160
+على نقطة و mix زي الـ blood grouping تمام؟ زي الـ
+
+200
+00:13:25,160 --> 00:13:28,060
+agglutination reactions لكن الحقيقة لما اتعمّقنا
+
+201
+00:13:28,060 --> 00:13:31,040
+فيه في الـ .. في الـ .. لجانا لإنه فيه الآن تقنيات
+
+202
+00:13:31,040 --> 00:13:35,620
+حديثة جدا .. جدا فيها magnetic particles و فيها
+
+203
+00:13:35,620 --> 00:13:40,200
+إليزا في الـ IMX يعني فيه تقنيات عالية جدا بتوضع
+
+204
+00:13:40,200 --> 00:13:44,660
+اللي هو الـ .. الـ .. الـ antibody reaction الفحص الـ
+
+205
+00:13:44,660 --> 00:13:49,890
+ESOT هذا .. كلكم بتعرفوا ما حد لو صار عنده
+
+206
+00:13:49,890 --> 00:13:56,250
+infection الآن والـ antibody بيطلع الآن بده وجد ما
+
+207
+00:13:56,250 --> 00:13:58,390
+بين الـ infection وما بين ظهور الـ antibody بيختلف
+
+208
+00:13:58,390 --> 00:14:00,950
+إذا كان الـ primary response إيش يعني primary
+
+209
+00:14:00,950 --> 00:14:04,850
+response؟ يعني لأول مرة بدخل الـ antigen بده حوالي
+
+210
+00:14:04,850 --> 00:14:09,410
+عشر أيام لإسبوعين بس إذا كان secondary response
+
+211
+00:14:09,410 --> 00:14:13,790
+ممكن يطلع خلال 48 ساعة الـ peak تبعه لإنه في memory
+
+212
+00:14:13,790 --> 00:14:17,990
+cell صح؟ في memory cell، طب إحنا قاعدين بنقول إن
+
+213
+00:14:17,990 --> 00:14:22,010
+معظمنا .. معظمنا أصيب يبقى فيه عندنا memory .. فيه
+
+214
+00:14:22,010 --> 00:14:26,290
+عندنا memory قد يكون هذا secondary response مش
+
+215
+00:14:26,290 --> 00:14:32,250
+primary response لكن في الآخر هو ليس مؤشر كمان مرة
+
+216
+00:14:32,250 --> 00:14:35,940
+على أن الـ infection لأن قد تذهب الـ infection .. قد
+
+217
+00:14:35,940 --> 00:14:38,480
+.. قد .. يعني يشفى الإنسان من المرض لكي يلاقي الـ
+
+218
+00:14:38,480 --> 00:14:41,220
+antibody .. الـ antibody إيه تبعه؟ مرتفع .. هذا
+
+219
+00:14:41,220 --> 00:14:44,000
+مؤشر على إيه؟ على إنه ممكن يصير عنده complication
+
+220
+00:14:44,000 --> 00:14:46,520
+.. إنه يكون عنده complication .. إيش معنى
+
+221
+00:14:46,520 --> 00:14:50,220
+complication على اللوحة هنا؟ clinical sequela ..
+
+222
+00:14:50,220 --> 00:14:55,520
+sequela اللي هي الـ .. اللي هي الـ complication طيب
+
+223
+00:14:55,520 --> 00:15:00,010
+.. وصلنا الآن متكلمين عن Staphylococcus aureus,
+
+224
+00:15:00,170 --> 00:15:02,710
+Epidermidis, Saprophyticus, Streptococcus
+
+225
+00:15:02,710 --> 00:15:06,090
+biogenes, Streptococcus agalactia, Enterococcus
+
+226
+00:15:06,090 --> 00:15:10,970
+واتكلمنا آخر شهرين عن الـ viridans group اتكلّم
+
+227
+00:15:10,970 --> 00:15:14,650
+معاها لـ streptococcus pneumonia ومن اسمها أو
+
+228
+00:15:14,650 --> 00:15:19,290
+pneumonia تعمل pneumonia خذوا بالكم زي ما قلت الـ
+
+229
+00:15:19,290 --> 00:15:23,910
+meningitis في مسببات كثيرة لـ Meningitis برضه
+
+230
+00:15:23,910 --> 00:15:27,030
+نيومونيا إلها مسببات كثيرة أحدها الـ
+
+231
+00:15:27,030 --> 00:15:30,470
+streptococcus pneumonia لكن عشان نميزها عن الآخرين
+
+232
+00:15:30,470 --> 00:15:37,230
+تسمى looper pneumonia من كلمة loop، loop فص، يعني
+
+233
+00:15:37,230 --> 00:15:41,490
+هي ممكن تعمل يعني التهاب كامل أو التهاب لفص كامل
+
+234
+00:15:41,490 --> 00:15:43,890
+في الرئة، تمام؟ فبنسمّيها looper pneumonia
+
+235
+00:15:47,430 --> 00:15:51,370
+الصورة اللي قدامنا هنا بتعطينا فكرة عن أن
+
+236
+00:15:51,370 --> 00:15:56,610
+البكتيريا مش كروية تمامًا البكتيريا الكروية
+
+237
+00:15:56,610 --> 00:15:59,610
+المفروض تكون بهذا الشكل لكن هي بتكون بهذا الشكل و
+
+238
+00:15:59,610 --> 00:16:03,070
+كأنها كرة زي كرة الـ American football تعرفوها
+
+239
+00:16:03,070 --> 00:16:06,290
+الكرة البيضاوية American football ملتصقين جانب بعض
+
+240
+00:16:06,290 --> 00:16:11,270
+ملتصقين تمامًا؟ وعليهم capsule لأنّه صفة من صفات
+
+241
+00:16:11,270 --> 00:16:16,040
+الستيتو كوكاس نيمونيا الأساسية أنه إلها capsule فهي
+
+242
+00:16:16,040 --> 00:16:21,680
+دبلوكوكاي gram-positive دبلوكوكاي gram-positive و
+
+243
+00:16:21,680 --> 00:16:26,540
+capsulated هذه نقطة كتير مهمة من الـ viral
+
+244
+00:16:26,540 --> 00:16:30,500
+instructors تبعونها الـ capsule وكلكوا بتعرفوا الـ
+
+245
+00:16:30,500 --> 00:16:33,920
+capsule اللي إحنا بدنا نتكلم عنه عن الـ capsule تبع
+
+246
+00:16:33,920 --> 00:16:37,940
+الـ testoscopic pneumonia عارفين كم صنف منها
+
+247
+00:16:37,940 --> 00:16:44,770
+serology مبني على الكبسول 84 صنفيعني الـ step 2
+
+248
+00:16:44,770 --> 00:16:48,410
+coccus pneumonia ممكن تصيبنا كم مرة؟ 84 مرة هذا
+
+249
+00:16:48,410 --> 00:16:51,010
+على فرض إن لو نصيبنا فيها مرة مش هنصيب فيها مرة
+
+250
+00:16:51,010 --> 00:16:55,010
+ثانية طبعًا على فرض لأنّ الـ immunity بنسمّيها strain
+
+251
+00:16:55,010 --> 00:16:59,470
+specific إيش هي strain specific؟ يعني مرتبطة بنوع 
+
+252
+00:16:59,470 --> 00:17:04,830
+الكبسول اللي موجودة فالكبسول بنستفيد منها شغل تاني
+
+253
+00:17:04,830 --> 00:17:09,950
+بنستفيد منها في الـ vaccination لما بدنا نعطي 
+
+254
+00:17:09,950 --> 00:17:15,180
+الناس vaccine بدنا .. بدنا نحط في الـ vaccine
+
+255
+00:17:15,180 --> 00:17:19,420
+الأنواع الأكثر شيوعا في المنطقة يعني مثلا احنا في
+
+256
+00:17:19,420 --> 00:17:23,100
+فلسطين إيش الأنواع الأكثر شيوعا؟ خمسة عشر، سبعة عشر،
+
+257
+00:17:23,100 --> 00:17:26,220
+عشرين، تسعة و ثلاثين و أربعة
+
+258
+00:17:26,220 --> 00:17:30,420
+و ستين يبقى هذه السلالات بدل ما أحط أربعة و ثمانين
+
+259
+00:17:30,420 --> 00:17:35,480
+نوع بحط فقط مين؟ السلالات اللي هي السلالات في المنطقة
+
+260
+00:17:35,480 --> 00:17:39,120
+و لما نعمل كبسول هذا واحد رقم اثنين عملية الـ
+
+261
+00:17:39,120 --> 00:17:42,970
+identification طب و أنا إذا أنا بدي اتعرف .. أنا 
+
+262
+00:17:42,970 --> 00:17:45,990
+إذا بدي اتعرف على الميكروبات الشائعة هل بدي اتعرف
+
+263
+00:17:45,990 --> 00:17:48,770
+عليها كده هيك من الهواء؟ مش لازم لما .. لما أقعد الـ
+
+264
+00:17:48,770 --> 00:17:51,830
+microbe افحصه و اشوف الـ stereotype بتاعه؟ يبقى ..
+
+265
+00:17:51,830 --> 00:17:54,350
+يبقى استفدت أنا شغلتين من الكبسول هنا، خلال
+
+266
+00:17:54,350 --> 00:17:57,870
+المختبر استفادت تعريف و استفادت إيش اللي هو في
+
+267
+00:17:57,870 --> 00:18:00,970
+عملية الـ vaccination طب البكتيريا إيش مستفيدة من
+
+268
+00:18:00,970 --> 00:18:07,090
+الكبسول؟ من إيش؟ من الـ Phagocytosis؟ من الـ Phagocytosis
+
+269
+00:18:07,090 --> 00:18:10,280
+، خذوا بالكم في مصطلح أنا أول مرة يمروا معانا
+
+270
+00:18:10,280 --> 00:18:14,720
+هيو على السبورة وين الـ mouse؟  Opsonizing Antibody
+
+271
+00:18:14,720 --> 00:18:18,920
+بتسمعوا بمصطلح Opsonization لما سمعتوش في مصطلح هذا
+
+272
+00:18:18,920 --> 00:18:24,240
+Opsonization طيب ركزوا معايا هي البكتيريا هي
+
+273
+00:18:24,240 --> 00:18:28,480
+البكتيريا وهي
+
+274
+00:18:28,480 --> 00:18:32,480
+الـ antibody هي الـ antibody الآن ال .. ال .. ال ..
+
+275
+00:18:32,480 --> 00:18:35,460
+البكتيريا دي لها capsule لو قلت الـ Phagocyte تاخدها
+
+276
+00:18:35,460 --> 00:18:38,640
+.. انتم عارفين الـ capsule لزجة أو اللي بتهرب منها
+
+277
+00:18:38,640 --> 00:18:43,400
+طيب لو افترضنا ان الـ antibody يكون antibody للكبسول هي
+
+278
+00:18:43,400 --> 00:18:45,960
+الـ antibody هذا الـ antigen binding site ارتبط
+
+279
+00:18:45,960 --> 00:18:48,920
+بالكبسول وفيه اللي يقول لي لزجة مش لزجة هذا عبارة
+
+280
+00:18:48,920 --> 00:18:52,660
+عن specific receptor أو receptor to receptor الأثر
+
+281
+00:18:52,660 --> 00:18:57,280
+في عندي antibody مرتبط بمين؟ بالكبسول الجهة الثانية
+
+282
+00:18:57,280 --> 00:19:03,820
+اسمها Fc بار Fc مين إلها إلها receptor الـ Macrophage
+
+283
+00:19:03,820 --> 00:19:08,800
+والـ Phagocyte صحيح إذا لو صار في ارتباط بين antibody
+
+284
+00:19:08,800 --> 00:19:13,220
+وبين بكتيريا الكبسول بطلت الكبسول قادرة على القيام
+
+285
+00:19:13,220 --> 00:19:16,760
+بإيه بوظيفتها للهروب لإن الـ antibody عمل bridge
+
+286
+00:19:16,760 --> 00:19:22,450
+جسر ما بين البكتيريا ما بين اللي هو الـ Phagocytosis أي
+
+287
+00:19:22,450 --> 00:19:25,770
+antibody بيقوم بهذه المهمة بيسميه Opsonizing
+
+288
+00:19:25,770 --> 00:19:29,550
+antibody العملية اسمها Opsonization عملية اسمها
+
+289
+00:19:29,550 --> 00:19:32,390
+Opsonization يعني Covering .. Covering the
+
+290
+00:19:32,390 --> 00:19:36,170
+bacteria with antibodies تمام؟ بتساعد
+
+291
+00:19:36,170 --> 00:19:43,870
+عملية اللي هو الـ Phagocytosis ثبت
+
+292
+00:19:43,870 --> 00:19:45,850
+بالدليل القاطع وهذا المثال اللي حكيته لكم يعني
+
+293
+00:19:45,850 --> 00:19:51,540
+أنه اشتغل بعد Griffith لما قلنا إنه حق الفرن بواحد
+
+294
+00:19:51,540 --> 00:19:55,220
+إله capsule و التاني ما عندهوش capsule هي على الـ
+
+295
+00:19:55,220 --> 00:19:57,400
+Streptococcus pneumonia اللي بنتحكي كنا كنا بنتحكي
+
+296
+00:19:57,400 --> 00:20:01,100
+على الـ Streptococcus pneumonia و قلنا إنه الـ non
+
+297
+00:20:01,100 --> 00:20:06,960
+capsulated strain هو non pathogenic أو زي ما هو
+
+298
+00:20:06,960 --> 00:20:10,300
+مكتوب على السبورة avirulent أي ما انتم عارفين
+
+299
+00:20:10,300 --> 00:20:15,010
+نافية يعني إيه إنه ما فيش لو virus هذا لا يعني أن
+
+300
+00:20:15,010 --> 00:20:17,870
+الكبسول هي الـ virulence الوحيد اللي موجود فيها
+
+301
+00:20:17,870 --> 00:20:22,390
+حاجة اسمها نيومولايسين الـ Neumolysin حط مقابل له
+
+302
+00:20:22,390 --> 00:20:26,550
+analogy اللي هو الـ Streptolysin هو أصلا Hemolysin
+
+303
+00:20:26,550 --> 00:20:30,850
+و الـ Streptolysin برضه Hemolysin هذاك Hemolysin
+
+304
+00:20:30,850 --> 00:20:34,430
+سمينا Streptolysin O أو Streptolysin S اللي بتنتج
+
+305
+00:20:34,430 --> 00:20:38,730
+منين؟ الـ Streptococcus pyogenes هذا سمينا
+
+306
+00:20:38,730 --> 00:20:41,870
+Neumolysin لأنه بتنتج الـ Streptococcus pneumoniae
+
+307
+00:20:43,260 --> 00:20:48,600
+وظيفته الأساسية أنه يعمل Lysis للـ Red Blood Cell
+
+308
+00:20:48,600 --> 00:20:52,720
+كيف؟ عن طريق الـ destruction للـ cell membrane بيعمل
+
+309
+00:20:52,720 --> 00:20:57,460
+destruction للـ cell membrane لكن .. له كمان وظيفة
+
+310
+00:20:57,460 --> 00:21:03,300
+ثانية احنا قلنا ال .. ال .. الـ Phagocyte لما
+
+311
+00:21:03,300 --> 00:21:09,820
+تتغذى على بكتيريا في حاجة يسموها Metabolic burst
+
+312
+00:21:11,090 --> 00:21:16,870
+يعني انفجار في الـ Metabolism عشان نقدر ندمر الـ
+
+313
+00:21:16,870 --> 00:21:20,890
+Invader الخلية بتقوم بمجموعة ضخمة من الـ
+
+314
+00:21:20,890 --> 00:21:24,130
+biochemical reaction to destroy بيسموه Metabolic
+
+315
+00:21:24,130 --> 00:21:27,270
+burst بيقول إن هذا التوكسين بيعمل Interference مع
+
+316
+00:21:27,270 --> 00:21:28,070
+الـ Metabolic burst
+
+317
+00:21:32,640 --> 00:21:36,500
+بتأثير سلبي طبعا التأثير بيكون لما اقول Interference
+
+318
+00:21:36,500 --> 00:21:43,580
+أنا بتكلم عن حاجة تدخل سلبي تدخل سلبي جاني
+
+319
+00:21:43,580 --> 00:21:48,320
+Neumolysin إيش اشتغل؟ شغلتين واحد إنه عمل
+
+320
+00:21:48,320 --> 00:21:52,680
+Interference للـ Metabolic burst أو Oxidative burst
+
+321
+00:21:52,680 --> 00:21:57,340
+و الثاني عمل Lysis للـ Red Blood Cell لكن لكن هذا
+
+322
+00:21:57,340 --> 00:22:00,940
+الـ Lysis كان Complete ولا Incomplete؟ Incomplete
+
+323
+00:22:00,940 --> 00:22:04,620
+لأنه عمل Incomplete Hemolysis فاكرينه؟ هي Alpha
+
+324
+00:22:04,620 --> 00:22:08,020
+Hemolytic لإن Hemolysin بيعمل Green Discoloration
+
+325
+00:22:08,020 --> 00:22:13,380
+طيب، في كمان نظرية بتقول في السطر نفسه إنه
+
+326
+00:22:13,380 --> 00:22:16,400
+Unusually Ciliated Epithelial Cells يعني في
+
+327
+00:22:16,400 --> 00:22:19,960
+Destruction مش لـ Red Blood Cell، كمان للخلايا
+
+328
+00:22:19,960 --> 00:22:23,220
+الظهارية، للخلايا الظهارية هتشوفوا مصائب من
+
+329
+00:22:23,220 --> 00:22:26,760
+البكتيريا، هذه البكتيريا مزعجة وإن كان وصفها
+
+330
+00:22:26,760 --> 00:22:30,620
+opportunistic طيب في حد مش مكتوبة تنتبهوا عليها
+
+331
+00:22:30,620 --> 00:22:36,580
+عشان نستوعب الكلام اللي بنحكيه بعدين احنا
+
+332
+00:22:36,580 --> 00:22:41,080
+الشعب الفلسطيني عندنا ثقافة الاستشهاد واحد بيعمل
+
+333
+00:22:41,080 --> 00:22:47,260
+عملية استشهادية هو حابب الموت ولا بيضحى بنفسه علشان
+
+334
+00:22:47,260 --> 00:22:52,240
+الآخرين بيضحى بنفسه علشان الآخرين البكتيريا هذه
+
+335
+00:22:52,240 --> 00:22:55,120
+الـ Streptococcus pneumoniae عندها حاجة تشبه انتم عارفين
+
+336
+00:22:55,120 --> 00:22:57,870
+إن في كتير بتتعلمون الشعب الفلسطيني ومن ضمنها
+
+337
+00:22:57,870 --> 00:23:02,930
+البكتيريا في عندك Autolysin Autolysin
+
+338
+00:23:02,930 --> 00:23:09,190
+هذه إذا واحد زعلها أو أباها أو حاول يقتلها
+
+339
+00:23:09,190 --> 00:23:12,930
+بتقوله لأ على مالك أنا بدبل حالي من حالي تضغط على
+
+340
+00:23:12,930 --> 00:23:18,270
+زر الانفجار وبتنفجر الآن بتكون تتخيلوا أن
+
+341
+00:23:18,270 --> 00:23:22,610
+البكتيريا مش واحدة البكتيريا ملايين في الجسم هذه
+
+342
+00:23:22,610 --> 00:23:28,200
+الواحدة أو المجموعة اللي موتت حالها هي ماتت صحيح و
+
+343
+00:23:28,200 --> 00:23:31,560
+خسرت .. خسرنا بعض الأفراد لكن بتعوضهم .. انتم
+
+344
+00:23:31,560 --> 00:23:34,320
+عارفين .. برضه الولادات يعني الحمد لله كثيرة
+
+345
+00:23:34,320 --> 00:23:42,460
+بتعوضهم إيش بتعمل موت الخلايا ده إيش بيعمل؟ بيشغل
+
+346
+00:23:42,460 --> 00:23:47,260
+جهاز المناعة .. بيشغل جهاز المناعة بمعنى آخر إنه
+
+347
+00:23:47,260 --> 00:23:51,240
+البكتيريا و هي حية مؤذية لجهاز المناعة و هي ميتة
+
+348
+00:23:51,240 --> 00:23:55,970
+أيضا مؤذية لجهاز المناعة كيف؟ أول حاجة انتم بتعرفوا
+
+349
+00:23:55,970 --> 00:23:58,150
+أنه هي Gram-positive يعني فيها Peptidoglycan مش
+
+350
+00:23:58,150 --> 00:24:02,650
+Lipopolysaccharide وفيها Teichoic acid بيقولنا الـ دي اه دي
+
+351
+00:24:02,650 --> 00:24:05,870
+Teichoic acid and Peptidoglycan fragments إيش
+
+352
+00:24:05,870 --> 00:24:09,310
+يعني Fragments؟ البقايا يعني طبعا البقايا اللي من
+
+353
+00:24:09,310 --> 00:24:15,410
+وين؟ من الخلية اللي ماتت من الخلية اللي ماتت وكمان
+
+354
+00:24:15,410 --> 00:24:20,510
+في حينها اللي هو الـ phosphorylcholine هذه كلها
+
+355
+00:24:20,510 --> 00:24:25,470
+مركبات موجودة في البكتيريا they can mediate the
+
+356
+00:24:25,470 --> 00:24:29,730
+mobilization of inflammatory cells خلية ماتت هل
+
+357
+00:24:29,730 --> 00:24:34,710
+في داعي إنه نجيب الدنيا كلها و نعمل عملية قتل من
+
+358
+00:24:34,710 --> 00:24:37,070
+أول و جديد للبكتيريا الميتة ما هي ميتة خالص تقع
+
+359
+00:24:37,070 --> 00:24:42,170
+الموضوع لكن واضح إن المركبات هذه بتجذب الـ
+
+360
+00:24:42,170 --> 00:24:45,910
+inflammatory response زي النقطة المعروفة اللي جاء
+
+361
+00:24:45,910 --> 00:24:50,490
+واحد اتصل فلسا في المطافي و قالوله بيتنا مولع
+
+362
+00:24:50,490 --> 00:24:54,830
+تعالوا فلمّا اجتوا المطافي كانوا الجيران انطفوا
+
+363
+00:24:54,830 --> 00:24:57,190
+المكان فتبع الحريق قالوله احنا جايين نطف يعني
+
+364
+00:24:57,190 --> 00:25:00,030
+بلاش كلام فارغ ولّا ولّا يعني بدنا .. ولّا وقع
+
+365
+00:25:00,030 --> 00:25:05,230
+بدنا نطف هدول اجوا .. اجوا على المكان و ملاقوش فيه
+
+366
+00:25:05,230 --> 00:25:08,930
+حريق لا أو البكتيريا ميتة بدهم يشتغلوا فبيصير في
+
+367
+00:25:08,930 --> 00:25:11,330
+activation للـ Complement للـ Complement إيش بتعمل
+
+368
+00:25:11,330 --> 00:25:13,310
+بدلا من تعمل Destruction .. ما فيش بكتير تعمل
+
+369
+00:25:13,310 --> 00:25:17,020
+Destruction ازازا بتعمل Destruction لمين؟ يبقى
+
+370
+00:25:17,020 --> 00:25:22,340
+معناه الحكي أن الخلايا لما ماتت قادت الجسم بأنه
+
+371
+00:25:22,340 --> 00:25:29,280
+عمل Inflammatory response ما كان له داعي إيش
+
+372
+00:25:29,280 --> 00:25:31,720
+المركب اللي احنا حكينا عنه اللي .. اللي بساعد هذا
+
+373
+00:25:31,720 --> 00:25:34,680
+العملية مش ممنوع مش مبادئ قدامنا Autolysin اسمه
+
+374
+00:25:34,680 --> 00:25:38,200
+Autolysin يعني فينا Neumolysin و فينا Auto
+
+375
+00:25:38,200 --> 00:25:43,620
+lysin كمان سلاح بنعت في حرف الـ C في الجملة بيقول 
+
+376
+00:25:43,620 --> 00:25:50,860
+إنه البكتيريا تمتلك IgA Protease أعتقد للي فاكر إن
+
+377
+00:25:50,860 --> 00:25:56,340
+احنا كنا من أهم الـ specific defenses على الـ mucous
+
+378
+00:25:56,340 --> 00:26:00,120
+membrane هو وجود secretory IgA لما البكتيريا تكون
+
+379
+00:26:00,120 --> 00:26:03,640
+جاية ومعاها السلاح المضاد اللي هو الـ IgA Protease
+
+380
+00:26:03,640 --> 00:26:07,640
+فالحاجة إنه useless هذا واحد، رقم اثنين it has
+
+381
+00:26:07,640 --> 00:26:14,270
+specific adhesins قادر على الارتباط من برضه بالـ ..
+
+382
+00:26:14,270 --> 00:26:16,710
+الـ .. الـ .. الـ epithelial tissue تبع الـ
+
+383
+00:26:16,710 --> 00:26:20,130
+respiratory tract لذا كل المؤهلات الآن موجودة
+
+384
+00:26:20,130 --> 00:26:23,070
+معاها IgA Protease عند القدرة على الـ attachment
+
+385
+00:26:23,070 --> 00:26:25,350
+معاها capture to escape the process of 
+
+386
+00:26:25,350 --> 00:26:29,570
+phagocytosis معاها neumolysin معاها الـ auto lysin
+
+387
+00:26:29,570 --> 00:26:33,710
+بس في شغل بنفهمها على الـ auto lysin إليها علاقة
+
+388
+00:26:33,710 --> 00:26:37,570
+بالمعمل أننا في الآخر مش هي أطباء لأننا في الآخر
+
+389
+00:26:37,570 --> 00:26:43,040
+بنشتغل في المعمل تخيلوا البكتيريا اللي مرة ذكرناها
+
+390
+00:26:43,040 --> 00:26:48,000
+وقلنا growth curve تبعها هو lag, log, stationary,
+
+391
+00:26:48,540 --> 00:26:54,560
+dead الـ X-axis هو الـ time قولنا لكم طالت انقصرت ..
+
+392
+00:26:54,560 --> 00:26:58,660
+العبارة .. أنا بقدر أقولها جيدًا طالت انقصرت حياتها
+
+393
+00:26:58,660 --> 00:27:04,540
+أي شكل العام لها، dead، ثلاثة طيب لما يكون بكتيريا
+
+394
+00:27:04,540 --> 00:27:08,820
+زي هذه حساسة، fragile وعندها autolyseme أنا 
+
+395
+00:27:08,820 --> 00:27:13,160
+قلت لكم، هتحرض من أي حاجة، تمام؟ يعني لو خلص الأكل
+
+396
+00:27:13,160 --> 00:27:19,520
+عندها برضه هتموت، كويس؟ فممكن تنمو خلال 24 ساعة في 
+
+397
+00:27:19,520 --> 00:27:23,180
+liquid media تنمو تعمل turbidity وبعدين تموت و
+
+398
+00:27:23,180 --> 00:27:26,510
+تروح الـ turbidity فتجيدي تطلع الـ culture إنتي الـ
+
+399
+00:27:26,510 --> 00:27:29,210
+blood culture وكيف بتحكمي عليها إنتي؟ بتحكمي عليها
+
+400
+00:27:29,210 --> 00:27:32,410
+من خلال interability اللي جيتيها clear بتقولي عنها
+
+401
+00:27:32,410 --> 00:27:34,910
+negative يبقى هذا false negative ولا true
+
+402
+00:27:34,910 --> 00:27:38,750
+negative؟ false negative فلهذا الأراضي بدنا ننتبه
+
+403
+00:27:38,750 --> 00:27:42,350
+أن في بعض الميكروفات زي الـ Streptococcus pneumoniae قد
+
+404
+00:27:42,350 --> 00:27:47,770
+تختفي قبل 24 ساعة وبالتالي بدكون عندنا سياسة في
+
+405
+00:27:47,770 --> 00:27:54,130
+المختبر إنه نكشف عن الـ cultures خلال فترة في الـ 24
+
+406
+00:27:54,130 --> 00:27:57,850
+ساعة مرة على الأقل في النص يمكن يكون فيه turbidity
+
+407
+00:27:57,850 --> 00:28:01,170
+وثاني يوم ما يكونش فيه turbidity ليش؟ لأن البكتيريا
+
+408
+00:28:01,170 --> 00:28:07,050
+هنا ماتت وبعدها اختفت طبعًا احنا افترضنا الصفحة هذه
+
+409
+00:28:07,050 --> 00:28:11,270
+أو اللوحة هذه اللي قدامنا للـ capsule هي capsule
+
+410
+00:28:11,270 --> 00:28:15,330
+باردة بمعنى آخر إنها موجودة بشكل واضح وهي مؤشرة
+
+411
+00:28:15,330 --> 00:28:20,750
+على أن البكتيريا مولودة أو من يمتلكها هو الـ viral
+
+412
+00:28:20,750 --> 00:28:22,610
+strain antifagocytic
+
+413
+00:28:25,160 --> 00:28:28,220
+carbohydrate, antigen واحنا قلنا إنه it varies
+
+414
+00:28:28,220 --> 00:28:34,020
+بمعنى إنه تختلف among the strains فيه إنه 84 نوع
+
+415
+00:28:34,020 --> 00:28:38,560
+حسب الـ .. الـ .. الـ antigenة الـ immunity قلنا عنها
+
+416
+00:28:38,560 --> 00:28:41,420
+zero type specific بمعنى لو وصف الإنسان قلنا فيه
+
+417
+00:28:41,420 --> 00:28:45,720
+zero type بياخد immunity فقط لهذا الـ zero type لكن
+
+418
+00:28:45,720 --> 00:28:50,490
+لا يحمي من الـ other zero types بالمناسبة دخل الخدمة
+
+419
+00:28:50,490 --> 00:28:54,550
+عندنا قبل يمكن تلت سنوات الـ vaccine للأطفال و
+
+420
+00:28:54,550 --> 00:28:59,330
+الصحيح منذ إدخاله يعني بدأت بعض الأمراض تتخف عند
+
+421
+00:28:59,330 --> 00:29:02,410
+الأطفال منها الـ otitis media ومنها الـ meningitis
+
+422
+00:29:02,410 --> 00:29:04,890
+caused by this organism البكتيريا ده سبب إيه؟ من
+
+423
+00:29:04,890 --> 00:29:08,310
+نجوم الـ meningitis أقول لكم بيهم من نجوم الـ
+
+424
+00:29:08,310 --> 00:29:12,150
+meningitis كده مرة؟ وتحفظتوهم؟ من يعد بيهم
+
+425
+00:29:12,150 --> 00:29:12,830
+الأربعة؟
+
+426
+00:29:18,390 --> 00:29:20,850
+بس يوم الامتحانات ده أكيد أدوات من الامتحانات صح؟
+
+427
+00:29:20,850 --> 00:29:23,610
+ده أكيد أدوات من الامتحانات ده أكيد أدوات من
+
+428
+00:29:23,610 --> 00:29:24,010
+الامتحانات ده أكيد أدوات من الامتحانات ده أكيد أدوات
+
+429
+00:29:24,010 --> 00:29:24,090
+من الامتحانات ده أكيد أدوات من الامتحانات ده أكيد
+
+430
+00:29:24,090 --> 00:29:24,650
+أدوات من الامتحانات ده أكيد أدوات من الامتحانات ده
+
+431
+00:29:24,650 --> 00:29:26,370
+أكيد أدوات من الامتحانات ده أكيد أدوات من الامتحانات
+
+432
+00:29:26,370 --> 00:29:30,010
+ده أكيد أدوات من الامتحانات ده أكيد أدوات من
+
+433
+00:29:30,010 --> 00:29:36,990
+الامتحانات ده أكيد أدوات من الامتحانات ده نقول لهم
+
+434
+00:29:36,990 --> 00:29:41,730
+capsule نقول لهم capsule الثلاثة ممكن نكشف عن
+
+435
+00:29:41,730 --> 00:29:46,770
+موجودهم في الـ CSF من خلال كمان الـ antigen كمان مرة
+
+436
+00:29:46,770 --> 00:29:49,470
+Streptococcus pneumoniae, Haemophilus influenza و
+
+437
+00:29:49,470 --> 00:29:52,770
+Neisseria meningitidis يمتلكوا الكثير من الصفات المشتركة
+
+438
+00:29:52,770 --> 00:29:56,870
+وإن كانوا بينتموا لمجموعات مختلفة تمامًا واحدة Gram
+
+439
+00:29:56,870 --> 00:30:01,730
+-positive oxide اللي هي الـ pneumonia الـ meningitis
+
+440
+00:30:01,730 --> 00:30:05,530
+أو الـ meningitis Gram-negative diplococci
+
+441
+00:30:06,510 --> 00:30:08,470
+الـ Haemophilus influenza في الـ ones that grow up negative بس اللي
+
+442
+00:30:08,470 --> 00:30:12,650
+عادي، مختلفين تمامًا ومع ذلك اللي هم صفات مشتركة مع
+
+443
+00:30:12,650 --> 00:30:17,710
+بعضهم البعض طيب المرة القادمة إن شاء الله بنبدأ
+
+444
+00:30:17,710 --> 00:30:20,490
+بالـ clinical finding كافي اليوم السلام عليكم ورحمة
+
+445
+00:30:20,490 --> 00:30:21,130
+الله وبركاته
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/siSk8ZzrN8Q_postprocess.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/siSk8ZzrN8Q_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..551ebb108d13ade394a87c940c8883ee54feb60e
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/siSk8ZzrN8Q_postprocess.srt
@@ -0,0 +1,1780 @@
+1
+00:00:20,540 --> 00:00:23,720
+بسم الله الرحمن الرحيم نكمل ال biogenic kokai
+
+2
+00:00:23,720 --> 00:00:26,960
+المحاضرة السابقة تحدثتنا عن ال striptokokas
+
+3
+00:00:26,960 --> 00:00:30,340
+biogens و ال striptokokas الجالكتيا و بدأنا
+
+4
+00:00:30,340 --> 00:00:34,020
+بالحديث عن ال group D striptokokai أخدنا صفة
+
+5
+00:00:34,020 --> 00:00:37,920
+مشتركة صفة مشتركة أنه كل ال group D striptokokai
+
+6
+00:00:37,920 --> 00:00:42,480
+can grow on a medium known as bile-is-clean agar و
+
+7
+00:00:42,480 --> 00:00:47,810
+قلنا أن الصفتينالgrowth in bile و ال hydrolysis of
+
+8
+00:00:47,810 --> 00:00:51,630
+asculine يجب أن يتوفروا في الميكروب حتى يستطيع انه
+
+9
+00:00:51,630 --> 00:00:54,350
+ينجح في هذا الاختبار انه يكون positive قادر على
+
+10
+00:00:54,350 --> 00:00:57,790
+النمو في ال bile و من ثم قادر على استغلال اللي هو
+
+11
+00:00:57,790 --> 00:01:03,950
+ال asculine إذا كانت الصفة موجودة فالبكتيرياgroup
+
+12
+00:01:03,950 --> 00:01:08,210
+D streptococci الان كيف نميز enterococcus عن non
+
+13
+00:01:08,210 --> 00:01:11,510
+enterococcus بواسطة ال growth او ال ability to
+
+14
+00:01:11,510 --> 00:01:16,170
+grow in 6.5 sodium chloride if positive it is
+
+15
+00:01:16,170 --> 00:01:20,790
+enterococcus if negative it is non enterococcus
+
+16
+00:01:20,790 --> 00:01:25,470
+كلمة non enterococcus ماحد سأل لماذا ندرس الان
+
+17
+00:01:25,470 --> 00:01:28,390
+group D streptococci وبنتكلم عن مين عن
+
+18
+00:01:28,390 --> 00:01:31,310
+enterococcus ما علاقة ال enterococcus بال
+
+19
+00:01:31,310 --> 00:01:32,490
+streptococcus
+
+20
+00:01:34,400 --> 00:01:38,760
+الجواب في اللوحة هذه They are distantly related to
+
+21
+00:01:38,760 --> 00:01:42,440
+other streptococcus يمكن أن تيرو كوكاس إلها قرابة
+
+22
+00:01:42,440 --> 00:01:46,040
+بعيدة مع ال streptococcus أيام ما درسنا احنا
+
+23
+00:01:46,040 --> 00:01:50,700
+microbiology كان اسمها streptococcusيعني كان ..
+
+24
+00:01:50,700 --> 00:01:53,440
+كنا بنقول Striptococcus fecalis اليوم بنقول
+
+25
+00:01:53,440 --> 00:01:57,500
+Enterococcus fecalis يبقى كانت وصلنا في يوم من
+
+26
+00:01:57,500 --> 00:02:00,440
+الأيام ايه؟ Striptococcus لأنه بتمتلك بعض الصفات
+
+27
+00:02:00,440 --> 00:02:02,800
+الشبيهة فيها من ضمنها انها Glandoster
+
+28
+00:02:02,800 --> 00:02:07,720
+Coccyonacetalase نيجاتيل كمان تشترك معاها في ال
+
+29
+00:02:07,720 --> 00:02:09,860
+antigens عشان احنا قولنا group A و group B و group
+
+30
+00:02:09,860 --> 00:02:12,340
+.. يعني معنا الحاجة ان هي تمتلك ال antigenات اللي
+
+31
+00:02:12,340 --> 00:02:17,310
+بتبقى Striptococcus لكن ..سميت entero لإنها موجودة
+
+32
+00:02:17,310 --> 00:02:20,310
+ك normal flora of the intestinal tract أو بيسموها
+
+33
+00:02:20,310 --> 00:02:23,510
+gut flora gut flora يعني intestinal tract flora
+
+34
+00:02:23,510 --> 00:02:27,690
+كونها gut flora خدوا بالكم أذكركم بالإشريشيا
+
+35
+00:02:27,690 --> 00:02:31,650
+كولاية الإشريشيا كولاية gut flora و لا لأ مقطوط و
+
+36
+00:02:31,650 --> 00:02:36,130
+بتسبب إيه؟ بتسبب urinary tract infection number
+
+37
+00:02:36,130 --> 00:02:38,810
+one cause of urinary tract infection كان الإشريشيا
+
+38
+00:02:38,810 --> 00:02:46,140
+كولاية ليش؟ لأنهكنا لقرب الجهاز البولي من اللي هو
+
+39
+00:02:46,140 --> 00:02:51,280
+فتحة الإخراج وهي كذلك أكثر عند النساء من عن من
+
+40
+00:02:51,280 --> 00:02:55,920
+اللي هو الذكور وبالتالي بتوقع أن ال enterofocus
+
+41
+00:02:55,920 --> 00:03:00,380
+كمان بتسبب urinary tract infection ناتجة عن مين؟
+
+42
+00:03:00,380 --> 00:03:04,520
+عن fecal contamination كمان بتسبب opportunistic
+
+43
+00:03:04,520 --> 00:03:09,090
+infectionوكلمة opportunistic ده يجب أن نتذكر أنه
+
+44
+00:03:09,090 --> 00:03:13,730
+بتصيب الناس اللي المناعة تبعتهم مُنخافرة انخفاض
+
+45
+00:03:13,730 --> 00:03:16,050
+المناعة بيكون غالبا وين؟ في أي مكان، في
+
+46
+00:03:16,050 --> 00:03:18,850
+المستشفيات، المرضى اللي موجودين في المستشفيات
+
+47
+00:03:18,850 --> 00:03:23,530
+وبالتالي بتوقع أن الميكروب هذا إيه له يعني خدنا
+
+48
+00:03:23,530 --> 00:03:28,130
+نقول انتشار واسع في المستشفيات أحد كمان أسباب
+
+49
+00:03:28,130 --> 00:03:31,990
+انتشاره الواسع مقدرة للبكتيريا اللي اسمها
+
+50
+00:03:31,990 --> 00:03:36,570
+Enterococcus على تكوين ال resistanceمن نجوم، مش
+
+51
+00:03:36,570 --> 00:03:39,290
+قولنا سنة في الكوكاس أولى سنة المرة الماضية، لسنة
+
+52
+00:03:39,290 --> 00:03:41,330
+الكوكاس بيرجينز ما قلنا عنها، إنها من نجوم ال
+
+53
+00:03:41,330 --> 00:03:44,830
+resistance، أما؟ ولا الأجلات كتير، لكن أنا بقول كل
+
+54
+00:03:44,830 --> 00:03:48,890
+يوم ال enterococcus هي من نجوم ال resistance،
+
+55
+00:03:48,890 --> 00:03:51,350
+المصطلح اللي أطلقته المرة الماضية على الفانكو
+
+56
+00:03:51,350 --> 00:03:56,460
+mycine تعالى نشوفهبس جابي نتطلع على الكلمة الأخيرة
+
+57
+00:03:56,460 --> 00:04:00,660
+في اللوحة Most Common Species Encountered in
+
+58
+00:04:00,660 --> 00:04:03,900
+Clinical Laboratory أو In Clinical Medicine أو In
+
+59
+00:04:03,900 --> 00:04:07,200
+Clinical Practice in Pterococcus Fecalis اللي هي
+
+60
+00:04:07,200 --> 00:04:10,460
+أخت تانية اسمها in Pterococcus Faecium والتنتين
+
+61
+00:04:10,460 --> 00:04:13,620
+بدلوا على نفس ال origin اللي هو الفيكل ال material
+
+62
+00:04:13,620 --> 00:04:19,500
+يعني من ال intestinal tract نتطلع على المصطلح اللي
+
+63
+00:04:19,500 --> 00:04:23,820
+أطلحناه المرة الماضية اللي هو ال VREVancomycin
+
+64
+00:04:23,820 --> 00:04:29,180
+-resistant enterococcin مش فيها أنها مصطلح تاني
+
+65
+00:04:29,180 --> 00:04:34,260
+ممتسلة resistant staphylococcus aureus M R S A
+
+66
+00:04:34,260 --> 00:04:39,780
+قلنا من المصطلحات المهمة جدا في عصرنا الحاليو
+
+67
+00:04:39,780 --> 00:04:42,580
+ذكرتلكم معلومة و قولت ان ال Vancomycin و اعتبر من
+
+68
+00:04:42,580 --> 00:04:45,460
+قبل مانعضة نصحة العالمية على انه هو ال .. هو ال ..
+
+69
+00:04:45,460 --> 00:04:48,860
+ال .. ال .. الطلقة الأخيرة في ترسانة الطب ضد ال
+
+70
+00:04:48,860 --> 00:04:51,780
+gram-booster-cocci و احنا هنا جالين بنلاحظ انه كان
+
+71
+00:04:51,780 --> 00:04:54,640
+Vancomycin resistant و انا بقولكم في القطاع اللذة
+
+72
+00:04:54,640 --> 00:04:58,740
+ال Vancomycin resistant in terokokai عالية جدا ..
+
+73
+00:04:58,740 --> 00:05:03,260
+قد تصل الى 60% .. الى 60% .. اه افرض .. ال .. ال
+
+74
+00:05:03,260 --> 00:05:07,240
+.. ال ..عملت ال resistance طبعا للي مش عارف
+
+75
+00:05:07,240 --> 00:05:10,100
+ميكانيزمه ب action بالتأكيد مش هعرف اللي هو ال
+
+76
+00:05:10,100 --> 00:05:13,740
+resistance لذا بده اوضح انا كيف بيشتغل ال
+
+77
+00:05:13,740 --> 00:05:16,020
+vancomycin لكي نعرف كيف بيصير اللي هو ال
+
+78
+00:05:16,020 --> 00:05:21,320
+resistance تركزوا معايا تكرين ال cell wall لجرام
+
+79
+00:05:21,320 --> 00:05:23,880
+بوستر كان فيه اندي multiple layers of
+
+80
+00:05:23,880 --> 00:05:28,900
+peptidoglycan صحيح؟ و ال multiple layers were
+
+81
+00:05:28,900 --> 00:05:33,980
+cross linked و ده short peptide cross linkedكلمة
+
+82
+00:05:33,980 --> 00:05:37,700
+cross-link وقلنالكم مهمة جدا بدون cross-link
+
+83
+00:05:37,700 --> 00:05:40,440
+الطبقات هزلة collapse علي بعضها و ال structure
+
+84
+00:05:40,440 --> 00:05:45,300
+بيكون ضيف جدا فعندما لا يتكول ال cross-link بيفشل
+
+85
+00:05:45,300 --> 00:05:49,860
+نظام السلوك ال vancomycin بيشتر على انه بيرتبط
+
+86
+00:05:49,860 --> 00:05:55,660
+بيرتبط بحاسمة D-alanine تمام؟ و بيمنع عملية تكون
+
+87
+00:05:55,660 --> 00:06:00,260
+اللي هو ال cross-link البكتيريا هي موجودة علي الله
+
+88
+00:06:00,620 --> 00:06:04,920
+الصبورة دي قلانين بتغير وبت .. البكتيرا بتحوله او
+
+89
+00:06:04,920 --> 00:06:08,900
+.. او تماله replacement بحزمة D-lactate الان ال
+
+90
+00:06:08,900 --> 00:06:12,580
+receptor تبع ال vancomycin بطل موجود اذا مافي
+
+91
+00:06:12,580 --> 00:06:16,140
+receptor له لن يستطيع ان يرتبط اذا لم يستطيع ان
+
+92
+00:06:16,140 --> 00:06:18,620
+يرتبط لن يقوم بوظيفته وبالتالي البكتيرا أصبحت
+
+93
+00:06:18,620 --> 00:06:26,960
+مقاومة ل ال vancomycin واضح انه من الميكروبات ال
+
+94
+00:06:26,960 --> 00:06:31,260
+.. ال ..اللي تعلمت بسرعة و نقلت خبرتها لزملائها في
+
+95
+00:06:31,260 --> 00:06:34,300
+العالم اللي هي ال enterococcus لذلك أصبح عندنا
+
+96
+00:06:34,300 --> 00:06:36,300
+مصطلح مشهور جدا اسمه vancomycin resistant
+
+97
+00:06:36,300 --> 00:06:41,300
+enterococcus تخيلوا يا بنات انه مافي بديل عن ال
+
+98
+00:06:41,300 --> 00:06:44,760
+vancomycin هو في بدائل بس احنا كان قبل فترة لكن
+
+99
+00:06:44,760 --> 00:06:48,120
+تخيلوا ان مصر انه مافي بديل او ان البدائل limited
+
+100
+00:06:48,120 --> 00:06:52,600
+او ان البدائل very expensive و ده كلام صحيحو واحد
+
+101
+00:06:52,600 --> 00:06:55,620
+عنده urinary tract infection .. simple urinary
+
+102
+00:06:55,620 --> 00:06:57,820
+tract infection اللي كنت انعليجها بأبسط ال
+
+103
+00:06:57,820 --> 00:07:00,760
+antibiotic و مش جدين أعليجها .. تتوقع إيش يصير ..
+
+104
+00:07:00,760 --> 00:07:04,970
+إيش يصير في المرضى؟الـ UTI هتصير الـ complication
+
+105
+00:07:04,970 --> 00:07:08,150
+هتعمل الـ Pyelonephritis التهيأب في الكيلة نفسها و
+
+106
+00:07:08,150 --> 00:07:10,450
+قد تصل إلى الدم تعمل الستاسيميا و قد تصل إلى ال
+
+107
+00:07:10,450 --> 00:07:13,290
+meningitis تعمل ال .. ال meningus تعمل ال
+
+108
+00:07:13,290 --> 00:07:18,050
+meningitis وبالتالي يموت المريض من infection بسيطة
+
+109
+00:07:18,050 --> 00:07:20,730
+كانت تعالج بسبب ال antibiotic لعدم موجود
+
+110
+00:07:20,730 --> 00:07:26,350
+antibiotic في عالم ضده وهذا هو ال worst scenario
+
+111
+00:07:26,350 --> 00:07:31,090
+المتوقع في العشرة أعوام القادمة لذلك بنلاقي أن كل
+
+112
+00:07:31,090 --> 00:07:34,570
+العالمومن ضمن مُنظمات الصحة العالمية ومن ضمن
+
+113
+00:07:34,570 --> 00:07:38,430
+مُنظمات دولية حتى الحكومات صارت تتكلم عن هذا
+
+114
+00:07:38,430 --> 00:07:40,290
+الموضوع وعن الموضوع الـ antimicrobial resistance
+
+115
+00:07:40,290 --> 00:07:44,450
+ان لازم نجيله حل نبطئ الظاهرة نعالج الظاهرة نبحث
+
+116
+00:07:44,450 --> 00:07:49,310
+عن بدائل جديدةو البحث عن بداية جديدة جاري العمل
+
+117
+00:07:49,310 --> 00:07:52,450
+فيها، أنا هتقولكوا عن بديل، فاكرين؟ ال .. ال .. ال
+
+118
+00:07:52,450 --> 00:07:56,970
+bacterophage إنه استخدم ال bacterophage في قتل ال
+
+119
+00:07:56,970 --> 00:08:00,090
+.. ال .. ال .. ال bacteria نعم، يعمل علاج لل
+
+120
+00:08:00,090 --> 00:08:03,290
+infection في كمان شغل تاني اسمها quorum sensing،
+
+121
+00:08:03,290 --> 00:08:06,390
+فاكرينها؟ إن البكتيريا بتتكلم مع بعضها و بتعرف أنش
+
+122
+00:08:06,390 --> 00:08:11,320
+هددها، لو أنا ..عملت نوع من ال .. ال .. ال .. ال
+
+123
+00:08:11,320 --> 00:08:15,740
+interference يعني خربت .. شووشت .. شووشت بمواد
+
+124
+00:08:15,740 --> 00:08:20,320
+كيموية أخرى على البكتيريا مثلا كانوا عددها مية و
+
+125
+00:08:20,320 --> 00:08:23,600
+.. و خلتها تفكر حالة إنها .. إنها مليار حاسيت انتش
+
+126
+00:08:23,600 --> 00:08:26,800
+إجلال ال violence factor هتنبه جاز المناعة و بتجز
+
+127
+00:08:26,800 --> 00:08:31,090
+المناعة بيقضي عليها لقاياففيه هناك بدائي كثيرة،
+
+128
+00:08:31,090 --> 00:08:33,230
+البحث عن new antibiotics, new compounds,
+
+129
+00:08:33,650 --> 00:08:36,830
+modification of antibiotics اللي موجودة أساساً، كل
+
+130
+00:08:36,830 --> 00:08:43,150
+هذه طرق قد تساهم، لكن واضح أنه we are losing the
+
+131
+00:08:43,150 --> 00:08:48,250
+battle، احنا بنخسر المعركة مع ال micro لحد الآن،
+
+132
+00:08:48,250 --> 00:08:52,990
+هذا اللي بيبدو ..مجموعة أخرى غير ال group D
+
+133
+00:08:52,990 --> 00:08:56,970
+streptococci اسمها viridans streptococci ال
+
+134
+00:08:56,970 --> 00:09:00,490
+viridans معناها the green streptococci وسمت ال
+
+135
+00:09:00,490 --> 00:09:03,610
+green لأنها alpha hemolytic يعني على الطبق على بطء
+
+136
+00:09:03,610 --> 00:09:07,090
+جهرش اللي كله منها أو المستعمرات حواليها هتكون
+
+137
+00:09:07,090 --> 00:09:13,250
+خضرة diverse species متنوعة قرال في السكن ال
+
+138
+00:09:13,250 --> 00:09:14,990
+habitat تبعتها في الفن
+
+139
+00:09:19,200 --> 00:09:22,180
+الأمراض اللي بتسببها it is alpha hemolytic and
+
+140
+00:09:22,180 --> 00:09:26,100
+negative for other tests ماعرفش يعني هذه جملة
+
+141
+00:09:26,100 --> 00:09:29,520
+غامضة لو واحد يعني بدي قرأها لأول مرة بالفعل هي
+
+142
+00:09:29,520 --> 00:09:34,280
+غامضة Alpha hemolytic مافي يعني خلاف and negative
+
+143
+00:09:34,280 --> 00:09:38,780
+for other tests negative for other tests that are
+
+144
+00:09:38,780 --> 00:09:41,240
+performed for the similar alpha hemolytic
+
+145
+00:09:41,240 --> 00:09:47,690
+streptococcus pneumoniaلما احنا بندور على صفات
+
+146
+00:09:47,690 --> 00:09:51,610
+تمييزية، بندور عليها لمين؟ لاتنين مختلفين ولا
+
+147
+00:09:51,610 --> 00:09:56,630
+لاتنين متشابهين؟ لاتنين متشابهين مش جادرين
+
+148
+00:09:56,630 --> 00:10:00,390
+يتمييزوا مع بعض، طب نحاول ندور على صفات، تفاصيل،
+
+149
+00:10:00,390 --> 00:10:02,310
+عشان يتميزوا مع بعض، ليش ده الحصانين الأبيض
+
+150
+00:10:02,310 --> 00:10:07,200
+والأسود؟ مافيش ده عنهم مظبوط تميز بينهمفانا عندي
+
+151
+00:10:07,200 --> 00:10:10,520
+الـ Stripped Caucas pneumonia و Stripped Caucas
+
+152
+00:10:10,520 --> 00:10:14,340
+viridance بشكل عام بيشبهوا بعض بينهم التنين ألفة
+
+153
+00:10:14,340 --> 00:10:18,080
+هيموريتك التنين دبلو كوكاية تنين دبلو كوكاية فكيف
+
+154
+00:10:18,080 --> 00:10:22,260
+نميزهم عن بعض؟ في few tests positive لل Stripped
+
+155
+00:10:22,260 --> 00:10:26,280
+Caucas pneumonia كلهم و negative لمن؟ لل viridance
+
+156
+00:10:26,280 --> 00:10:29,400
+group فلما نتعلم ال test تبعه Stripped Caucas
+
+157
+00:10:29,400 --> 00:10:34,740
+pneumonia هنفهم الجملة هذه وان هي negative it is
+
+158
+00:10:34,740 --> 00:10:37,860
+non-groupableو الـ non-grover برضه صفة تشترك فيها
+
+159
+00:10:37,860 --> 00:10:41,260
+مع مين؟ مع الـ streptococcus pneumonia صح؟ التنتين
+
+160
+00:10:41,260 --> 00:10:45,780
+non-grover it includes streptococcus mutants اللي
+
+161
+00:10:45,780 --> 00:10:51,060
+ممكن تسبب endocarditis خلال عملية خلاء الأسنان أو
+
+162
+00:10:51,060 --> 00:10:56,260
+الطحون لما واحد بيخلع سن أو طحونة برضه عملية عنيفة
+
+163
+00:10:56,260 --> 00:11:00,780
+يا بنات و فيها تمذيق للأنسجة و بينزل دم بكميات
+
+164
+00:11:00,780 --> 00:11:05,100
+كبيرة مش زي أسنان الحليب و اللبن تمام؟
+
+165
+00:11:07,430 --> 00:11:11,150
+فبالتالي الفم كلكم عارفين هو بيحتوي على عدد كبير
+
+166
+00:11:11,150 --> 00:11:14,930
+من الميكروبينزين و هذه البكتيريا oral امكانية انه
+
+167
+00:11:14,930 --> 00:11:21,170
+يصل مايكروبات الى الدم عالية جدا وبالتالي الطباء
+
+168
+00:11:21,170 --> 00:11:25,370
+الأسنان بيعطونا antibiotics البعض مابيلتزم اما لا
+
+169
+00:11:25,370 --> 00:11:28,350
+بيلتزمش بالجرعة او بيلتزمش بالcourse نفسه او
+
+170
+00:11:28,350 --> 00:11:33,440
+مابياخدش من أساسه antibioticsفقد تنتقل الـ
+
+171
+00:11:33,440 --> 00:11:37,060
+streptococcus mutants من الفم إلى الدم إلى القلب
+
+172
+00:11:37,060 --> 00:11:40,480
+وتسبب إنه ما يسمى بالـ endocarditis ال .. ال ..
+
+173
+00:11:40,480 --> 00:11:45,920
+العصبورة شرحناه ولا شرحناوش هذا؟ ASOT Anti
+
+174
+00:11:45,920 --> 00:11:48,160
+Streptolicein O أذكركوا فيه بس على السريع
+
+175
+00:11:48,160 --> 00:11:53,800
+Streptolicein O S O S O Streptolicein O بما أنه هو
+
+176
+00:11:53,800 --> 00:11:59,510
+الهيموليسين اللي في الـ streptococcusو بيحلل الدم،
+
+177
+00:11:59,510 --> 00:12:03,370
+لكن أحد مزاياه أنه لو اتعرض للأكسيدين بتدمر، سمناه
+
+178
+00:12:03,370 --> 00:12:07,530
+Oxidin Lapide Stryptomycin O ال Antibody اللي
+
+179
+00:12:07,530 --> 00:12:12,430
+بيطلعله سمناه Antistryptomycin O ASO
+
+180
+00:12:13,930 --> 00:12:17,290
+لمّا اتفقنا إن معظمنا أصيب في يوم من الأيام ..
+
+181
+00:12:17,290 --> 00:12:20,030
+أصيب بماذا؟ بالـ tonsillitis، يعني بـ
+
+182
+00:12:20,030 --> 00:12:22,530
+streptococcus pyidins، يعني في عندنا antibodies
+
+183
+00:12:22,530 --> 00:12:26,830
+معظمنا لهذه البكتيريا، سواء كنا الآن مرضى أو
+
+184
+00:12:26,830 --> 00:12:30,770
+ماكننا مرضى، فلو عملنا فحص، للكل حلّا جيب
+
+185
+00:12:30,770 --> 00:12:33,090
+positive، فكيبنا الميز بينه .. بين recent
+
+186
+00:12:33,090 --> 00:12:35,650
+infection و بين previous infection فعملنا
+
+187
+00:12:35,650 --> 00:12:39,520
+الـtiter، الـtiter معناها نقياسمقياس تركيز ال
+
+188
+00:12:39,520 --> 00:12:43,520
+antibodies في الجسم إذا كان فوق المتان فهو recent
+
+189
+00:12:43,520 --> 00:12:48,220
+إذا أقل من المتان فهو negative فحص Antigen
+
+190
+00:12:48,220 --> 00:12:53,110
+Antibody reaction إيش بنسميه؟ بنجوسان سيرولوجييبقى
+
+191
+00:12:53,110 --> 00:12:57,150
+فحوصات ال serology هي عبارة عن فحوصات معتمدة على
+
+192
+00:12:57,150 --> 00:13:00,250
+.. في ال reaction تبعها على ثلاث عناصر، ايش عبارة
+
+193
+00:13:00,250 --> 00:13:03,530
+عن ثلاث عناصر؟ ال antigen، ال antibody و ال
+
+194
+00:13:03,530 --> 00:13:06,590
+complement، فدول التلاتة تقريبا هم ال .. طبعا في
+
+195
+00:13:06,590 --> 00:13:09,510
+.. في أشكال و ألوان في ال .. في ال serology، لكن
+
+196
+00:13:09,510 --> 00:13:12,530
+في الآخر هي العناصر الرئيسية، antigen, antibody
+
+197
+00:13:12,530 --> 00:13:16,770
+and complement احنا كنا احنا طلاب نقول عنه علم
+
+198
+00:13:16,770 --> 00:13:21,080
+النبتطولوجياليش علم التنكتولوجي؟ لأننا بنحط نقطة
+
+199
+00:13:21,080 --> 00:13:25,160
+على نقطة و mix زي ال blood grouping تمام؟ زي ال
+
+200
+00:13:25,160 --> 00:13:28,060
+agglutination reactions لكن الحقيقة لما اتعمقنا
+
+201
+00:13:28,060 --> 00:13:31,040
+فيه في ال .. في ال .. لجانا لأ إنه فيه الآن تقنيات
+
+202
+00:13:31,040 --> 00:13:35,620
+حديثة جدا .. جدا فيها magnetic particles و فيها
+
+203
+00:13:35,620 --> 00:13:40,200
+إليزا في ال IMX يعني فيه تقنيات عالية جدا بتوضع
+
+204
+00:13:40,200 --> 00:13:44,660
+اللي هو ال .. ال .. ال antibody reaction الفحص ال
+
+205
+00:13:44,660 --> 00:13:49,890
+ESOT هذا ..كلكم بتعرفوا ما حد لو صير عنده
+
+206
+00:13:49,890 --> 00:13:56,250
+infection الآن و ال antibody بطلع الآن بده وجد ما
+
+207
+00:13:56,250 --> 00:13:58,390
+بين ال infection و ما بين ظهور ال antibody بيختلف
+
+208
+00:13:58,390 --> 00:14:00,950
+إذا كان ال primary response إيش يعني primary
+
+209
+00:14:00,950 --> 00:14:04,850
+response؟ يعني لأول مرة بدخل ال antigen بده حوالي
+
+210
+00:14:04,850 --> 00:14:09,410
+عشر أيام لإسبوعين بس إذا كان secondary response
+
+211
+00:14:09,410 --> 00:14:13,790
+ممكن يطلع خلال 48 ساعة ال peak تبعه لإنه في memory
+
+212
+00:14:13,790 --> 00:14:17,990
+cell صح؟ في memory cellطب احنا قاعدين بنقول ان
+
+213
+00:14:17,990 --> 00:14:22,010
+معظمنا .. معظمنا أصيب يبقى فيه عندنا memory .. فيه
+
+214
+00:14:22,010 --> 00:14:26,290
+عندنا memory قد يكون هذا secondary response مش
+
+215
+00:14:26,290 --> 00:14:32,250
+primary response لكن في الآخر هو ليس مؤشر كمان مرة
+
+216
+00:14:32,250 --> 00:14:35,940
+على ان ال infection لانقد تذهب ال infection .. قد
+
+217
+00:14:35,940 --> 00:14:38,480
+.. قد .. يعني يشفى الإنسان من المرض لكي يلاقي ال
+
+218
+00:14:38,480 --> 00:14:41,220
+antibody .. ال antibody إيه تبعه؟ مرتفع .. هذا
+
+219
+00:14:41,220 --> 00:14:44,000
+مؤشر على إيه؟ على إنه ممكن يصير عنده complication
+
+220
+00:14:44,000 --> 00:14:46,520
+.. إنه يكون عنده complication .. إيش معنى
+
+221
+00:14:46,520 --> 00:14:50,220
+complication على اللوحة هنا؟ clinical sequela ..
+
+222
+00:14:50,220 --> 00:14:55,520
+sequela اللي هي ال .. اللي هي ال complication طيب
+
+223
+00:14:55,520 --> 00:15:00,010
+..صلنا الآن متكلمين عن Staphylococcus aureus,
+
+224
+00:15:00,170 --> 00:15:02,710
+Epidermidis, Saprophyticus, Streptococcus
+
+225
+00:15:02,710 --> 00:15:06,090
+biogenes, Streptococcus agalactia, Enterococcus
+
+226
+00:15:06,090 --> 00:15:10,970
+واتكلمنا آخر شهرين عن ال viridans group اتكلم
+
+227
+00:15:10,970 --> 00:15:14,650
+معاها ل streptococcus pneumonia و من اسمها او
+
+228
+00:15:14,650 --> 00:15:19,290
+pneumonia تعمل pneumonia اخدوا بالكم زي ما قلت ال
+
+229
+00:15:19,290 --> 00:15:23,910
+meningitisفي مساببات كثيرة لـ Meningitis برضه
+
+230
+00:15:23,910 --> 00:15:27,030
+نيومونيا إلها مساببات كثيرة أحدهم الـ
+
+231
+00:15:27,030 --> 00:15:30,470
+streptococcus pneumonia لكن عشان نميزها عن الأخرين
+
+232
+00:15:30,470 --> 00:15:37,230
+تسمى looper pneumonia من كلمة loop، loop فص، يعني
+
+233
+00:15:37,230 --> 00:15:41,490
+هي ممكن تعمل يعني التهاب كامل أو التهاب لفص كامل
+
+234
+00:15:41,490 --> 00:15:43,890
+في الرؤى، تمام؟ فبنسميها looper pneumonia
+
+235
+00:15:47,430 --> 00:15:51,370
+الصورة اللي قدامنا هنا بتعطينا فكرة عن أن
+
+236
+00:15:51,370 --> 00:15:56,610
+البكتيريا مش كراوية تماما البكتيريا الكراوية
+
+237
+00:15:56,610 --> 00:15:59,610
+المفروض تكون بهذا الشكل لكن هي بتكون بهذا الشكل و
+
+238
+00:15:59,610 --> 00:16:03,070
+كأنها كرة زي كرة ال American football تعرفوها
+
+239
+00:16:03,070 --> 00:16:06,290
+الكرة البيضاوية American football تمتن جانب بعض
+
+240
+00:16:06,290 --> 00:16:11,270
+ملازجين تمام؟ و عليهم capsule لأنه صفة من صفات
+
+241
+00:16:11,270 --> 00:16:16,040
+الستيتو كوكاس نيمونيا الأساسية أنه إلها capsuleفهي
+
+242
+00:16:16,040 --> 00:16:21,680
+دبلوكوكاي gram-postif دبلوكوكاي gram-postif و
+
+243
+00:16:21,680 --> 00:16:26,540
+capsulated هذه نقطة كتير مهمة من ال viral
+
+244
+00:16:26,540 --> 00:16:30,500
+instructors تبعونها ال capsule و كلكوا بتعرف ال
+
+245
+00:16:30,500 --> 00:16:33,920
+capsule اللي انا بدنا نتكلم عنه عن ال capsule تبع
+
+246
+00:16:33,920 --> 00:16:37,940
+ال testoscopic pneumonia عارفين أكم صنف منها
+
+247
+00:16:37,940 --> 00:16:44,770
+serology مبني على الكبسول 84 صنفيعني ال step 2
+
+248
+00:16:44,770 --> 00:16:48,410
+coccus pneumonia ممكن تصيبنا كام مرة؟ 84 مرة هذا
+
+249
+00:16:48,410 --> 00:16:51,010
+على فرض إن لو نصيبنا فيها مرة مش هنصيب فيها مرة
+
+250
+00:16:51,010 --> 00:16:55,010
+تانية طبعا على فرض لإن ال immunity بنسميها strain
+
+251
+00:16:55,010 --> 00:16:59,470
+specific إيش هي strain specific؟ يعني مرتبطة بنوع
+
+252
+00:16:59,470 --> 00:17:04,830
+الكبسول اللي موجودة فالكبسول بنستفيد منها شغل تاني
+
+253
+00:17:04,830 --> 00:17:09,950
+بنات بنستفيد منها في ال vaccination لما بدنا نعطي
+
+254
+00:17:09,950 --> 00:17:15,180
+الناس vaccineبدنا .. بدنا نحط في ال vaccine
+
+255
+00:17:15,180 --> 00:17:19,420
+الأنواع الأكثر شيوعا في المنطقة يعني مثلا أحنا في
+
+256
+00:17:19,420 --> 00:17:23,100
+فلسطين إيش الأنواع الأكثر شيوعا؟ خمستاشر، سبعتاشر،
+
+257
+00:17:23,100 --> 00:17:26,220
+عشرين، تسعة وتمانين و .. عفوا تسعة وتلاتين و أربعة
+
+258
+00:17:26,220 --> 00:17:30,420
+و ستين يبقى هذه السلالات بدل ما أحط أربعة و تمانين
+
+259
+00:17:30,420 --> 00:17:35,480
+نوع بحط فقط مين؟ الشيعة اللي هو الشيعة في المنطقة
+
+260
+00:17:35,480 --> 00:17:39,120
+و بلما نوم كابسو هذا واحد رقم اتنين عملية ال
+
+261
+00:17:39,120 --> 00:17:42,970
+identificationطب و انا اذا انا بدي اتعرف .. انا
+
+262
+00:17:42,970 --> 00:17:45,990
+اذا بدي اتعرف الميكروبات الشائعة هل بدي اتعرف
+
+263
+00:17:45,990 --> 00:17:48,770
+عليها كده هيك من الهوى؟ مش لازم لما .. لما اقعد ال
+
+264
+00:17:48,770 --> 00:17:51,830
+microbe افحصه و اشوف ال stereotype بتاعه؟ يبقى ..
+
+265
+00:17:51,830 --> 00:17:54,350
+يبقى استفادت انا شغلتان من الكابسول هنا، خلال
+
+266
+00:17:54,350 --> 00:17:57,870
+المختبر استفادت تعريف و استفادت ايش اللي هو في
+
+267
+00:17:57,870 --> 00:18:00,970
+عملية ال vaccination طب البكتيريا ايش مستفيدة من
+
+268
+00:18:00,970 --> 00:18:07,090
+الكابسول؟ من ايش؟ من الفاغو؟ cytos؟ من الفاغو
+
+269
+00:18:07,090 --> 00:18:10,280
+cytos، خدوا بالكمفي مصطلح انا اول مرة يمروا معانا
+
+270
+00:18:10,280 --> 00:18:14,720
+هيوا على السبورة وين ال mouse? Obsonic Antibody
+
+271
+00:18:14,720 --> 00:18:18,920
+تسمع بمصطلح Obsonization لما سمعتوش في مصطلح هذا
+
+272
+00:18:18,920 --> 00:18:24,240
+Obsonization طيب ركزوا معايا هي البكتيريا هي
+
+273
+00:18:24,240 --> 00:18:28,480
+البكتيريا وهي
+
+274
+00:18:28,480 --> 00:18:32,480
+ال antibody هي ال antibodyالان ال .. ال .. ال ..
+
+275
+00:18:32,480 --> 00:18:35,460
+البكتيريا دي لها capsule لو قلت الفاجو سايت تاخدها
+
+276
+00:18:35,460 --> 00:18:38,640
+.. انتوا عارفين ال capsule لزجة او اللي بتهرب منها
+
+277
+00:18:38,640 --> 00:18:43,400
+طيب لو افترضنا ان الاسم يكون antibody للكبسول هى
+
+278
+00:18:43,400 --> 00:18:45,960
+ال antibody هذا ال antigen binding site ارتبط
+
+279
+00:18:45,960 --> 00:18:48,920
+بالكبسول وفيش اللي يقوللي لزجة مش لزجة هذا عبارة
+
+280
+00:18:48,920 --> 00:18:52,660
+عن specific receptor او receptor to receptor الأثر
+
+281
+00:18:52,660 --> 00:18:57,280
+في عندي antibody مرتبط بمين؟بالكبسول الجهة التانية
+
+282
+00:18:57,280 --> 00:19:03,820
+اسمها Fc بار Fc مين إله إلها receptor الماكروفاج
+
+283
+00:19:03,820 --> 00:19:08,800
+والفاجو صحيح إذا لو صار في ارتباط بين antibody
+
+284
+00:19:08,800 --> 00:19:13,220
+وبين بكتيريا الكبسول بطلت الكبسول قادرة على قيام
+
+285
+00:19:13,220 --> 00:19:16,760
+بإيه بوظيفتها للهروب لإن ال antibody عمل bridge
+
+286
+00:19:16,760 --> 00:19:22,450
+جسر ما بينالبكتيريا مبين اللي هو الفاجو سايتوس أي
+
+287
+00:19:22,450 --> 00:19:25,770
+antibody بيقوم بهذه المهمة بيسميه optionizing
+
+288
+00:19:25,770 --> 00:19:29,550
+antibody العملية اسمها optionization عملية اسمها
+
+289
+00:19:29,550 --> 00:19:32,390
+optionization يعني covering .. covering the
+
+290
+00:19:32,390 --> 00:19:36,170
+bacteria with antibodies تمام؟ بتسا .. بتساعد
+
+291
+00:19:36,170 --> 00:19:43,870
+عملية اللي هو الفاجو سايتوس ثبت
+
+292
+00:19:43,870 --> 00:19:45,850
+بالدليل القاطع وهذا المثال اللي حكيها لكوا يعني
+
+293
+00:19:45,850 --> 00:19:51,540
+انهتشتر بعد Griffith لما قلنا إنه حق الفرن بواحد
+
+294
+00:19:51,540 --> 00:19:55,220
+إله capsule و التاني ماعرفوش capsule هي على ال
+
+295
+00:19:55,220 --> 00:19:57,400
+streptococcus pneumonia اللي بنتحكي كنا كنا بنتحكي
+
+296
+00:19:57,400 --> 00:20:01,100
+على ال streptococcus pneumonia و قلنا إنه ال non
+
+297
+00:20:01,100 --> 00:20:06,960
+capsulated strain هو non pathogenic أو زي ما هو
+
+298
+00:20:06,960 --> 00:20:10,300
+مكتوب على الصبورة a virulent اي ما أنتوا عارفين
+
+299
+00:20:10,300 --> 00:20:15,010
+نافية يعني إيه إنه مافيش لو virusهذا لا يعني أن
+
+300
+00:20:15,010 --> 00:20:17,870
+الكبسول هي ال virulence الوحيد اللي موجود فيها
+
+301
+00:20:17,870 --> 00:20:22,390
+حاجة اسمها نيومولايسين النيومولايسين حط مقابل له
+
+302
+00:20:22,390 --> 00:20:26,550
+analogy اللي هو ال streptolicein هو أصلا هيموليسين
+
+303
+00:20:26,550 --> 00:20:30,850
+و ال streptolicein برضه هيموليسين هداك هيموليسين
+
+304
+00:20:30,850 --> 00:20:34,430
+سمنا streptolicein O أو streptolicein S اللي بتنتج
+
+305
+00:20:34,430 --> 00:20:38,730
+منين؟ ال streptococcus بيوجينز هذا سمناه
+
+306
+00:20:38,730 --> 00:20:41,870
+نيومولايسين لأنه بتنتج ال streptococcus النيوموني
+
+307
+00:20:43,260 --> 00:20:48,600
+وظيفته الأساسية أنه يعمل Lyses لل Red Blood Cell
+
+308
+00:20:48,600 --> 00:20:52,720
+كيف؟ عن طريق ال destruction لل cell membrane بيعمل
+
+309
+00:20:52,720 --> 00:20:57,460
+destruction لل cell membrane لكن .. له كمان وظيفة
+
+310
+00:20:57,460 --> 00:21:03,300
+تانية احنا قولنا ال .. ال .. ال phagocyte لما
+
+311
+00:21:03,300 --> 00:21:09,820
+تتغذى على بكتيريا في حاجة يسموها metabolic burst
+
+312
+00:21:11,090 --> 00:21:16,870
+يعني انفجار في ال Metabolism عشان نقدر ندمر ال
+
+313
+00:21:16,870 --> 00:21:20,890
+Invaler الخلية بتقوم بمجموعة ضخمة من ال
+
+314
+00:21:20,890 --> 00:21:24,130
+biochemical reaction to destroy بيسموه metabolic
+
+315
+00:21:24,130 --> 00:21:27,270
+burst بيقول إن هذا التوكسين بيعمل interference مع
+
+316
+00:21:27,270 --> 00:21:28,070
+ال metabolic burst
+
+317
+00:21:32,640 --> 00:21:36,500
+بشوش سلبا طبعا التشوش بكون لما اقول interference
+
+318
+00:21:36,500 --> 00:21:43,580
+انا بتكلم عن حاجة تدخل سلبي تدخل سلبي جاني
+
+319
+00:21:43,580 --> 00:21:48,320
+مولاييسين ايش اشتغل؟ شغلتين واحد انه عمل
+
+320
+00:21:48,320 --> 00:21:52,680
+interference لل metabolic burst او oxidative burst
+
+321
+00:21:52,680 --> 00:21:57,340
+والتاني عمل license لل red blood cell لكن لكن هذا
+
+322
+00:21:57,340 --> 00:22:00,940
+ال license كان complete ولا incomplete؟incomplete
+
+323
+00:22:00,940 --> 00:22:04,620
+لإنه عمل incomplete hemolysis فاكرينه؟ هي Alpha
+
+324
+00:22:04,620 --> 00:22:08,020
+Hemolytic لإن Hemolysin بيعمل Green Discoloration
+
+325
+00:22:08,020 --> 00:22:13,380
+طيب، في كمان نظرية بتقول في السطر نفسه إنه
+
+326
+00:22:13,380 --> 00:22:16,400
+Unpossibly Ciliated Epithelial Cells يعني في
+
+327
+00:22:16,400 --> 00:22:19,960
+Destruction مش لRed Blood Cell، كمان للخلايا
+
+328
+00:22:19,960 --> 00:22:23,220
+الطلاقى، للخلايا الطلاقى هتشوفوا مصائب من
+
+329
+00:22:23,220 --> 00:22:26,760
+البكتيريا، هذه البكتيريا مزعجة وإن كان وصفها
+
+330
+00:22:26,760 --> 00:22:30,620
+opportunisticطيب فى حد مش مكتوبة تنتبهوا عليها
+
+331
+00:22:30,620 --> 00:22:36,580
+عشان نستوعب الكلام اللى بنحكيه بعدك احنا
+
+332
+00:22:36,580 --> 00:22:41,080
+الشعب الفلسطينى عندنا ثقافة الاستشهاد واحد بيعمل
+
+333
+00:22:41,080 --> 00:22:47,260
+عملية استشهادية هو حاب الموت ولا بيضحى بنفسه علشان
+
+334
+00:22:47,260 --> 00:22:52,240
+الآخرين بيضحى بنفسه علشان الآخرين البكتيريا هذه
+
+335
+00:22:52,240 --> 00:22:55,120
+الستراتوكوكاس نيموني عندها حاجة تشبه فانتوا عارفين
+
+336
+00:22:55,120 --> 00:22:57,870
+ان في كتير بتعلمون الشعب الفلسطينىومن ضمنها
+
+337
+00:22:57,870 --> 00:23:02,930
+البكتيريا في عندك حاسوب اوتو لايسين اوتو لايسين
+
+338
+00:23:02,930 --> 00:23:09,190
+هذه إذا واحد زعلها ولا أباها ولا حاول يقتلها
+
+339
+00:23:09,190 --> 00:23:12,930
+بتقوله لأ على مالك أنا بدبّل حالي من حالي تضغط على
+
+340
+00:23:12,930 --> 00:23:18,270
+زر الإنفجار وبتنفجر الآن بتكون تتخيلوا أن
+
+341
+00:23:18,270 --> 00:23:22,610
+البكتيريا مش واحدة البكتيريا ملايين في الجسم هذه
+
+342
+00:23:22,610 --> 00:23:28,200
+الواحدة أو المجموعة اللي موتت حالهاهي ماتت صحيح و
+
+343
+00:23:28,200 --> 00:23:31,560
+خسرت .. خسرنا بعض الأفراد لكن بتعوضهم .. انتوا
+
+344
+00:23:31,560 --> 00:23:34,320
+عارفين .. برضه الولادات يعني الحمد لله كثيرة
+
+345
+00:23:34,320 --> 00:23:42,460
+بتعوضهم إيش بتعمل موت الخلايا ده إيش بيعمل؟ بيشغل
+
+346
+00:23:42,460 --> 00:23:47,260
+جهاز المناعة .. بيشغل جهاز المناعة بمعنى أخر إنه
+
+347
+00:23:47,260 --> 00:23:51,240
+البكتيريا و هي حية مؤذية جهاز المناعة و هي ميتة
+
+348
+00:23:51,240 --> 00:23:55,970
+أيضا مؤذية جهاز المناعة كيف؟ أول حاجةانتوا بتعرفوا
+
+349
+00:23:55,970 --> 00:23:58,150
+انه هي ground positive يعني فيها بيبتدوا ..مش
+
+350
+00:23:58,150 --> 00:24:02,650
+lycan وفيها Tychoic acid بيقولنا ال .. دي اه دي
+
+351
+00:24:02,650 --> 00:24:05,870
+Tychoic acid and بيبتدوا ب lycan fragments ايش
+
+352
+00:24:05,870 --> 00:24:09,310
+يعني fragments؟ البقايا يعني طبعا البقايا اللي من
+
+353
+00:24:09,310 --> 00:24:15,410
+وين؟ من الخلية اللي ماتت من الخلية اللي ماتت وكمان
+
+354
+00:24:15,410 --> 00:24:20,510
+في حينها اللي هو ال phosphoryl chlorine هذه كلها
+
+355
+00:24:20,510 --> 00:24:25,470
+مركباتموجودة في البكتيريا they can mediate the
+
+356
+00:24:25,470 --> 00:24:29,730
+mobilization of inflammatory cells خليها ماتت هل
+
+357
+00:24:29,730 --> 00:24:34,710
+في داعي إنه نجيب الدنيا كلها و نعمل عملية قتل من
+
+358
+00:24:34,710 --> 00:24:37,070
+أول و جديد للبكتيريا الميتة ما هي ميتة خالص تقع
+
+359
+00:24:37,070 --> 00:24:42,170
+الموضوع لكن واضح إن المركبات هذه بتجذب ال
+
+360
+00:24:42,170 --> 00:24:45,910
+inflammatory response زي النقطة المعروفة اللي جاء
+
+361
+00:24:45,910 --> 00:24:50,490
+واحداتصل فلسا فى المطافى وقالوله بيتنا مولّا
+
+362
+00:24:50,490 --> 00:24:54,830
+تعالوا فلمّا اجتوا المطافى كانوا الجيران انطفين
+
+363
+00:24:54,830 --> 00:24:57,190
+المكان فتبع الحريقه قالوله احنا جايين نطف يعنى
+
+364
+00:24:57,190 --> 00:25:00,030
+بلاش كلام فارق ولّا ولّا يعني بدنا .. ولّا وقع
+
+365
+00:25:00,030 --> 00:25:05,230
+بدنا نطف هدوله اجوا .. اجوا على المكان وملاقوش فيه
+
+366
+00:25:05,230 --> 00:25:08,930
+حريقه لأو البكتيره ميته بدهم يشتغلوا فبصير فى
+
+367
+00:25:08,930 --> 00:25:11,330
+activation ال compliment ال compliment ايش بتعمل
+
+368
+00:25:11,330 --> 00:25:13,310
+بدلا من تعمل destruction .. مافيش بكتير تعمل
+
+369
+00:25:13,310 --> 00:25:17,020
+destruction أزازا بتعمل destruction لمين؟يبجى
+
+370
+00:25:17,020 --> 00:25:22,340
+معناه الحكي أن الخلايا لما ماتت قادت الجسم بأنه
+
+371
+00:25:22,340 --> 00:25:29,280
+عملت inflammatory response ما كان له داعي إيش
+
+372
+00:25:29,280 --> 00:25:31,720
+المركب اللي أحنا حكينا عنه اللي .. اللي بساعد هذا
+
+373
+00:25:31,720 --> 00:25:34,680
+العملية مش ممنوع مش مبادخر قدامنا auto lysine اسمه
+
+374
+00:25:34,680 --> 00:25:38,200
+auto lysine يعني فينا neumolysine و فينا auto
+
+375
+00:25:38,200 --> 00:25:43,620
+lysineكمان سلاح بنعت في حرف ال C في الجملة بيقول
+
+376
+00:25:43,620 --> 00:25:50,860
+انه البكتيريا تمتلك IgA Protease اعتقد للي فاكر ان
+
+377
+00:25:50,860 --> 00:25:56,340
+احنا كنا من اهم ال specific defenses على ال mucous
+
+378
+00:25:56,340 --> 00:26:00,120
+membrane هو وجود secretory IgA لما البكتيريا تكون
+
+379
+00:26:00,120 --> 00:26:03,640
+جاية و معاها السلاح المضاد اللي هو ال IgA Protease
+
+380
+00:26:03,640 --> 00:26:07,640
+فالحاجة انه useless هذا واحد رقم اتنين it has
+
+381
+00:26:07,640 --> 00:26:14,270
+specific adhesinsقادر على الارتباط من برضه بال ..
+
+382
+00:26:14,270 --> 00:26:16,710
+ال .. ال .. ال epithelial tissue تبع ال
+
+383
+00:26:16,710 --> 00:26:20,130
+respiratory tract لذا كل المؤهلات الآن موجودة
+
+384
+00:26:20,130 --> 00:26:23,070
+معاها IgA Protease عند القدرة على ال attachment
+
+385
+00:26:23,070 --> 00:26:25,350
+معاها capture to escape the process of
+
+386
+00:26:25,350 --> 00:26:29,570
+phagocytosis معاها neumolysin معاها ال auto lysin
+
+387
+00:26:29,570 --> 00:26:33,710
+بس في شغل بنفهمها على ال auto lysin إليها علاقة
+
+388
+00:26:33,710 --> 00:26:37,570
+بالمعمل أننا في الآخر مش هي طباء لأننا في الآخر
+
+389
+00:26:37,570 --> 00:26:43,040
+بنشتغل في المعملتخيلوا البكتيريا اللى مرة ذكرناها
+
+390
+00:26:43,040 --> 00:26:48,000
+وقلنا growth curve تبعها هو lag, log, stationary,
+
+391
+00:26:48,540 --> 00:26:54,560
+dead ال X-axis هو ال time قولنالكوا طالة انقصرت ..
+
+392
+00:26:54,560 --> 00:26:58,660
+العبارة .. أنا بقدر أقولها جيدا طالت انقصرت حياتها
+
+393
+00:26:58,660 --> 00:27:04,540
+أي شكل العام لها، dead، تلاتة طيب لما يكون بكتيريا
+
+394
+00:27:04,540 --> 00:27:08,820
+زي هذه حساسة، fragile و عندها autolysemeأنا
+
+395
+00:27:08,820 --> 00:27:13,160
+قولتلك، هتحرض من أي حاجة، تمام؟ يعني لو خلص الأكل
+
+396
+00:27:13,160 --> 00:27:19,520
+عندها برضه هتموت، كويس؟ فممكن تنمو خلال 24 ساعة في
+
+397
+00:27:19,520 --> 00:27:23,180
+liquid media تنمو تعمل turbidity و بعدين تموت و
+
+398
+00:27:23,180 --> 00:27:26,510
+تروح ال turbidityفتجيدي تطلع ال culture انتي ال
+
+399
+00:27:26,510 --> 00:27:29,210
+blood culture وكيف بتحكم عليها انتي؟ بتحكم عليها
+
+400
+00:27:29,210 --> 00:27:32,410
+من خلال interability اللي جيتيها clear بتقول عنها
+
+401
+00:27:32,410 --> 00:27:34,910
+negative يبجي هذا false negative ولا true
+
+402
+00:27:34,910 --> 00:27:38,750
+negative؟ false negative فليهذا الأراضي بدنا ننتبه
+
+403
+00:27:38,750 --> 00:27:42,350
+ان في بعض الميكروفات زي الاستراتيكوكاس نيمونيا قد
+
+404
+00:27:42,350 --> 00:27:47,770
+تختفي قبل 24 ساعة وبالتالي بدكون عندنا سياسة في
+
+405
+00:27:47,770 --> 00:27:54,130
+المختبر انه نكشف عن ال cultures خلال فترةفي الـ 24
+
+406
+00:27:54,130 --> 00:27:57,850
+ساعة مرة على الأقل في النص يمكن يكون فيه turbidity
+
+407
+00:27:57,850 --> 00:28:01,170
+و تاني يوم مايكونش فيه turbidity ليش؟ لأن البكتيرا
+
+408
+00:28:01,170 --> 00:28:07,050
+هنا مات و بعدها اختفت طبعا احنا افرضنا الصفحة هذه
+
+409
+00:28:07,050 --> 00:28:11,270
+او اللوحة هذه اللي قدامنا لل capsule هي capsule
+
+410
+00:28:11,270 --> 00:28:15,330
+باردة بمعنى اخر انها موجودة بشكل واضح وهي مؤشرة
+
+411
+00:28:15,330 --> 00:28:20,750
+على ان البكتيرا مولودة او من يمتلكها هو ال viral
+
+412
+00:28:20,750 --> 00:28:22,610
+strain antifagocytic
+
+413
+00:28:25,160 --> 00:28:28,220
+carbohydrate, antigen واحنا قلنا انه it varies
+
+414
+00:28:28,220 --> 00:28:34,020
+بمعنى انه تختلف among the strains فيه انه 84 نوع
+
+415
+00:28:34,020 --> 00:28:38,560
+بحسب ال .. ال .. ال antigenة ال immunity قلنا عنها
+
+416
+00:28:38,560 --> 00:28:41,420
+zero type specific بمعنى لو وصف الانسان قلنا فيه
+
+417
+00:28:41,420 --> 00:28:45,720
+zero type بياخد immunity فقط لهذا ال zero type لكن
+
+418
+00:28:45,720 --> 00:28:50,490
+لا يحمي من ال other zero typesبالمناسبة دخل الخدمة
+
+419
+00:28:50,490 --> 00:28:54,550
+عندنا قبل يمكن تلت سنوات ال vaccine للأطفال و
+
+420
+00:28:54,550 --> 00:28:59,330
+الصحيح منذ إدخاله يعني بدأت بعض الأمراض تتخف عند
+
+421
+00:28:59,330 --> 00:29:02,410
+الأطفال منها ال otitis media و منها ال meningitis
+
+422
+00:29:02,410 --> 00:29:04,890
+caused by this organism ال بكتيره ده سبب إيه؟ من
+
+423
+00:29:04,890 --> 00:29:08,310
+نجوم ال meningitis أقولك بيهم من نجوم ال
+
+424
+00:29:08,310 --> 00:29:12,150
+meningitis كده مرة؟ و تحفظتوهم؟ من يعد بيهم
+
+425
+00:29:12,150 --> 00:29:12,830
+الأربعة؟
+
+426
+00:29:18,390 --> 00:29:20,850
+بس يوم الامتحانات ده اكيد ادوة من الامتحانات صح؟
+
+427
+00:29:20,850 --> 00:29:23,610
+ده اكيد ادوة من الامتحانات ده اكيد ادوة من
+
+428
+00:29:23,610 --> 00:29:24,010
+الامتحانات ده اكيد ادوة من الامتحانات ده اكيد ادوة
+
+429
+00:29:24,010 --> 00:29:24,090
+من الامتحانات ده اكيد ادوة من الامتحانات ده اكيد
+
+430
+00:29:24,090 --> 00:29:24,650
+ادوة من الامتحانات ده اكيد ادوة من الامتحانات ده
+
+431
+00:29:24,650 --> 00:29:26,370
+اكيد ادوة من الامتحانات ده اكيد ادوة من الامتحانات
+
+432
+00:29:26,370 --> 00:29:30,010
+ده اكيد ادوة من الامتحانات ده اكيد ادوة من
+
+433
+00:29:30,010 --> 00:29:36,990
+الامتحانات ده اكيد ادوة من الامتحانات ده انقولهم
+
+434
+00:29:36,990 --> 00:29:41,730
+capsule نقولهم capsule التلاتة ممكن نكشف عن
+
+435
+00:29:41,730 --> 00:29:46,770
+موجودهم في ال CSF من خلال كمان ال antigen كمان مرة
+
+436
+00:29:46,770 --> 00:29:49,470
+Stratococcus pneumonia, Hemophilus influenza و
+
+437
+00:29:49,470 --> 00:29:52,770
+Neisseria meia يمتلكوا الكثير من الصفات المشتركة
+
+438
+00:29:52,770 --> 00:29:56,870
+وإن كانوا بينتموا لمجموعات مختلفة تماما واحدة Gram
+
+439
+00:29:56,870 --> 00:30:01,730
+-bostric oxide اللي هي ال pneumonia ال meningitis
+
+440
+00:30:01,730 --> 00:30:05,530
+أو ال meningitis Gram-negative diplococci
+
+441
+00:30:06,510 --> 00:30:08,470
+الهموفلسين في الones that grow up negative بس اللي
+
+442
+00:30:08,470 --> 00:30:12,650
+عادي، مختلفين تماما ومع ذلك اللي هم صفات مشتركة مع
+
+443
+00:30:12,650 --> 00:30:17,710
+بعضهم البعض طيب المرة القادمة ان شاء الله بنبدأ
+
+444
+00:30:17,710 --> 00:30:20,490
+بال clinical finding كاف اليوم السلام عليكم ورحمة
+
+445
+00:30:20,490 --> 00:30:21,130
+الله وبركاته
+
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/u5P7UmriOkc_postprocess.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/u5P7UmriOkc_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..51b6b92391b84b3aaa83742a22aeff063b36625c
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/u5P7UmriOkc_postprocess.srt
@@ -0,0 +1,2488 @@
+1
+00:00:21,580 --> 00:00:25,060
+بسم الله الرحمن الرحيم هنا المحاضرة الماضي توقفنا
+
+2
+00:00:25,060 --> 00:00:29,100
+عند أحد أعضاء ال .. أو العضو الأساسي للحركة
+
+3
+00:00:29,100 --> 00:00:33,080
+البكتيريا اللي هو الفلاجلة واتفقنا على أنه مكون من
+
+4
+00:00:33,080 --> 00:00:36,060
+تلت أجزاء رئيسية اللي هي ال basal body أو ال motor
+
+5
+00:00:36,060 --> 00:00:40,200
+اللي هو المحرك وكان embedded في ال cell membrane
+
+6
+00:00:40,200 --> 00:00:44,760
+وكان فيروس اسمه ال hook اللي هو خارج من ال cell
+
+7
+00:00:44,760 --> 00:00:47,440
+membrane إلى خارج الخلية يعني مختلف عن ال cell
+
+8
+00:00:47,440 --> 00:00:50,670
+wall و بعدين ال filament اللي هو الخيط الطويلبعدين
+
+9
+00:00:50,670 --> 00:00:55,010
+تطرقنا لتوزيعات مختلفة لفلاجلة شفنا mono-trichas,
+
+10
+00:00:55,590 --> 00:00:59,690
+lovo-trichas, amphitrichas و peritrichas بحسب
+
+11
+00:00:59,690 --> 00:01:02,650
+توزيع الفلاجلة و قلنا إنه مهم جدا نعرف إن البكتيرا
+
+12
+00:01:02,650 --> 00:01:08,250
+متحركة أو غير متحركةلماذا هذه الأهمية في ال
+
+13
+00:01:08,250 --> 00:01:12,970
+microbiology؟ نظرا نقولكوا أن عملية التعرف على
+
+14
+00:01:12,970 --> 00:01:15,750
+البكيرة بتتم من خلال صفات إيجابية و صفات سلبية
+
+15
+00:01:15,750 --> 00:01:18,870
+فبنعطي مثال بسيط على هذا الموضوع ال motility
+
+16
+00:01:18,870 --> 00:01:23,570
+بالذات، هناك Salmonella و هناك Shigella، اسمين
+
+17
+00:01:23,570 --> 00:01:26,230
+اقترنوا بعض، لما بنقول Salmonella، غالبا على طول
+
+18
+00:01:26,230 --> 00:01:29,590
+وراها تلقائي بيجي موضوع الشجلة، Salmonella and
+
+19
+00:01:29,590 --> 00:01:36,160
+Shigellaهذا بالاطباط الوثيق يعني .. يعني الشبه ..
+
+20
+00:01:36,160 --> 00:01:41,060
+الشبه بين ال two organism أحد أهم الفروقات اللى
+
+21
+00:01:41,060 --> 00:01:43,940
+بنستخدمها بالتفريق بينهم ال motility ال salmonella
+
+22
+00:01:43,940 --> 00:01:48,190
+motile والshigilanone motileإذا ال motility
+
+23
+00:01:48,190 --> 00:01:51,690
+بالنسبة لنا في ال microbiology مهمة لإنها بتميزنا
+
+24
+00:01:51,690 --> 00:01:55,910
+بين البكتيريا نفسها إنها متحركة و غير متحركة طب
+
+25
+00:01:55,910 --> 00:01:58,870
+هنا كمان أهمية أخرى في البكتيريا المتحركة ما أحنا
+
+26
+00:01:58,870 --> 00:02:02,210
+شوفنا قبل شوية في الرسم أو المحاضرة السابقة إنه في
+
+27
+00:02:02,210 --> 00:02:04,990
+بعض البكتيريا المتحركة بتكون متحركة ب one single
+
+28
+00:02:04,990 --> 00:02:08,390
+polar flagella زي Sodomonas aeruginosa و زي ال
+
+29
+00:02:08,390 --> 00:02:13,030
+Vibrio cholera هاي مثل على اتنين بكتيريا مشهورة
+
+30
+00:02:13,030 --> 00:02:17,040
+بتحركة ب .. بflagella واحدةبينما الـ
+
+31
+00:02:17,040 --> 00:02:20,420
+Enterobacteriasi اللي بينها الإشريش الكولاي المفضل
+
+32
+00:02:20,420 --> 00:02:25,100
+ليداكم تتحرك ب peritricous flagella إذن توزيع
+
+33
+00:02:25,100 --> 00:02:27,900
+الفلاجلة في البكتيريا أيضًا يساعدنا في التعرف على
+
+34
+00:02:27,900 --> 00:02:31,460
+نوع هذه البكتيريا إذن أول نقطة على اللوحة اللي
+
+35
+00:02:31,460 --> 00:02:35,360
+قدامكم إنه it is used in the identification of
+
+36
+00:02:35,360 --> 00:02:38,780
+بكتيريا تستخدم في التعريف بالنسبة للبكتيريا it
+
+37
+00:02:38,780 --> 00:02:44,000
+contributes to بعض الgenesis كيف اتفقنا إن هي تهرب
+
+38
+00:02:44,000 --> 00:02:44,300
+من
+
+39
+00:02:47,030 --> 00:02:51,370
+المخاطر وتذهب إلى الغنى وبالتالي هذا يعطيها أفضلية
+
+40
+00:02:51,370 --> 00:02:55,230
+على البكتيرى الغير متحرك طبعا احنا قلنا ان هي ال
+
+41
+00:02:55,230 --> 00:03:01,070
+organ ل ال motility فيه البكتيرى نجي للتركيب الآخر
+
+42
+00:03:01,070 --> 00:03:07,690
+يسمه P-Lie ال P-Lie عبارة عن hair-like projections
+
+43
+00:03:07,690 --> 00:03:08,990
+يعني زوايا تشبه
+
+44
+00:03:13,550 --> 00:03:16,510
+لأ مش الهداب، الهداب اسمها celia والبكتيريا ..
+
+45
+00:03:16,510 --> 00:03:20,730
+والبكتيريا لابد بها celia التراكيب الخاص الصحيح هو
+
+46
+00:03:20,730 --> 00:03:23,730
+.. هنتعرف عليها ان شاء الله على صنفين منها الصنف
+
+47
+00:03:23,730 --> 00:03:27,690
+الأول بيسموه common pili أو phembria common pili
+
+48
+00:03:27,690 --> 00:03:31,650
+هي في قدامك وشايفينها هنا common pili أو phembria
+
+49
+00:03:31,650 --> 00:03:35,710
+وفينا ال six pili اللي هي ال .. ال pili المتعلقة
+
+50
+00:03:35,710 --> 00:03:38,730
+بالجنس الأولى اللي هي ال common pili أو ال
+
+51
+00:03:38,730 --> 00:03:42,750
+phembria عبارة عن فعن، فعن يعني أيه؟ رفيعة، rigid
+
+52
+00:03:43,560 --> 00:03:47,220
+adhesion rigid يعني فيها نوع من إيه؟ من الصلابة
+
+53
+00:03:47,220 --> 00:03:51,540
+numerous متعددة عديدة related to bacterial
+
+54
+00:03:51,540 --> 00:03:55,900
+adhesion هيتاني structure ندرسه إيه له علاقة
+
+55
+00:03:55,900 --> 00:04:01,040
+بالالتصاق أو الارتباط بالبكتيريا وهو ال ال ال ال
+
+56
+00:04:01,040 --> 00:04:04,160
+ال بالاية الأول كان ال capsule الأول كان ال
+
+57
+00:04:04,160 --> 00:04:09,870
+capsuleإذا الفيمبريا ضرورية جدا لعملية اللي هو ال
+
+58
+00:04:09,870 --> 00:04:13,210
+attachment نجي للتانية اللي هو ال six-pillar وهي
+
+59
+00:04:13,210 --> 00:04:16,130
+عبارة عن longer أطول يعني في ال .. في ال .. في ال
+
+60
+00:04:16,130 --> 00:04:21,210
+size و courser .. courser يعني أخمر أسمك غالبا
+
+61
+00:04:21,210 --> 00:04:25,150
+بتكون موجودة بعدد قليل من واحد إلى أربعة وغالبا
+
+62
+00:04:25,150 --> 00:04:28,270
+الوظيفة الأساسية تبعتها هو ال conjugation ال
+
+63
+00:04:28,270 --> 00:04:31,750
+conjugation عبارة عن إطلان جنسي ودعونا ننظر الآن
+
+64
+00:04:31,750 --> 00:04:39,860
+إلى animation بتوضح هذا التركيبفي .. بشكل واضح قبل
+
+65
+00:04:39,860 --> 00:04:43,100
+ما نتكلم عن ال conjugation خلينا نتكلم على اللي هو
+
+66
+00:04:43,100 --> 00:04:47,880
+ال .. ال .. ال flushing away of بكتيريا اللي
+
+67
+00:04:47,880 --> 00:04:49,480
+مالهاش pili
+
+68
+00:04:56,340 --> 00:05:02,940
+طبعا هذا نفترض أنه هو الحاجب تمام الإنسان وإن
+
+69
+00:05:02,940 --> 00:05:07,000
+البكتيرى الآن صعدة بدها تروح تعمل infection في
+
+70
+00:05:07,000 --> 00:05:11,840
+الكدن قطرة بول واحد تعمل بتنزلهم لإنه مافيش هنا
+
+71
+00:05:11,840 --> 00:05:15,500
+attachment للأسف هذه هي عبارة عن كرتون صورة متحركة
+
+72
+00:05:15,500 --> 00:05:19,820
+لكن بنظام المعين الموجود في الحاسوب هذا مش ظاهر
+
+73
+00:05:19,820 --> 00:05:24,140
+إنها animated هنزلكوا يوم شير على ال facebook و
+
+74
+00:05:24,140 --> 00:05:25,580
+تشوفوه الآن الصورة التانية
+
+75
+00:05:32,930 --> 00:05:36,130
+بتوضح البكتيريا اللى عندها مقدرة على ال attachment
+
+76
+00:05:36,130 --> 00:05:40,810
+بواسطة الفمبريا أو ال common plyحتى لو قطرة
+
+77
+00:05:40,810 --> 00:05:43,790
+اليوران اجت او اليوران اجت بيبقى ثابتة في هذا
+
+78
+00:05:43,790 --> 00:05:47,450
+المكان، هذا ال attachment بيقولنا احد اهم العمليات
+
+79
+00:05:47,450 --> 00:05:50,790
+اللي بتساهم في ال successful colonization في
+
+80
+00:05:50,790 --> 00:05:56,310
+البرنامج، لان هحاول نشوف ال conjugation
+
+81
+00:06:03,600 --> 00:06:08,540
+in conjugation DNA is transferred from one living
+
+82
+00:06:08,540 --> 00:06:13,140
+bacteria يبدو أن الصوت مش تمام خلّيني أتكلم على
+
+83
+00:06:13,140 --> 00:06:17,840
+اللوحة اللي أمامنا شايفين بكتيريا ترتبط ببكتيريا
+
+84
+00:06:17,840 --> 00:06:23,860
+أخرى صفاتهم مختلفين الآن ال plasmid بدأت تنتقل
+
+85
+00:06:23,860 --> 00:06:29,610
+نسخة منها single و راحت للخلية التانيةالخلية
+
+86
+00:06:29,610 --> 00:06:35,510
+التانية اكتسبت صفات الخلية الأولى وبالتمثيل احنا
+
+87
+00:06:35,510 --> 00:06:41,850
+قد نستطيع ان نقول ان الأولى هي ال male والتانية هي
+
+88
+00:06:41,850 --> 00:06:46,370
+ال female لكن بعد اكتساب الصفة ال female بتصير
+
+89
+00:06:46,370 --> 00:06:50,010
+male وبالتالي مافيش بالمفهوم اللي احنا بنعرفه male
+
+90
+00:06:50,010 --> 00:06:54,110
+و female في البكتيريا وانما وجود الصفة انت تتقل
+
+91
+00:06:54,110 --> 00:06:58,360
+الى الخلية الأخرى فتكسبها هذه الصفةطبعا هذه
+
+92
+00:06:58,360 --> 00:07:01,700
+العملية راحظوا بتاعها بتعطي نسخة one single strand
+
+93
+00:07:01,700 --> 00:07:04,880
+بيصير له double strand هناك والأولى بتكون double
+
+94
+00:07:04,880 --> 00:07:09,900
+strand طبعا العملية قد بأكملها ممكن نمثلها بهذا
+
+95
+00:07:09,900 --> 00:07:16,180
+الشكل اللي أمامكواهذه المربعة F positive يعني فيها
+
+96
+00:07:16,180 --> 00:07:19,660
+صفة fertility ارتبطت ب F negative ال F negative
+
+97
+00:07:19,660 --> 00:07:23,980
+الآن على جهة وين ال mouse بدأت الآن في التصاق وثيق
+
+98
+00:07:23,980 --> 00:07:27,280
+بينهم راح على المربعة التالت صار في عملية انتقال
+
+99
+00:07:27,280 --> 00:07:31,820
+للمادة الوراثية من ال plasmid ومن ثم البشيرة ال F
+
+100
+00:07:31,820 --> 00:07:35,380
+negative صار F positive وبدأت تويد هي ال cycle
+
+101
+00:07:35,380 --> 00:07:40,460
+أخرى مع F negative cell هذه قضيةnormal conjugation
+
+102
+00:07:40,460 --> 00:07:44,000
+normal conjugation ان البلازميد هي اللي فقط بتنتقل
+
+103
+00:07:44,000 --> 00:07:48,980
+في نوع آخر من ال conjugation بيسموه HFR high
+
+104
+00:07:48,980 --> 00:07:53,580
+frequency recombination هذا عملية رهيبة جدا
+
+105
+00:07:53,580 --> 00:07:57,760
+هنشوفها الآن يا عزيزي تتبولة كويس شايفين وين
+
+106
+00:07:57,760 --> 00:08:00,920
+البلازميد؟ مفيش البلازميد البلازميد وين موجودة؟
+
+107
+00:08:00,920 --> 00:08:04,600
+البلازميد ارتطط بالكرومزوم ارتطط بمين؟ بالكرومزوم
+
+108
+00:08:05,290 --> 00:08:10,550
+ارتباطها بالكروموزوم يجعل من الكروموزوم كله أرضة
+
+109
+00:08:10,550 --> 00:08:17,170
+للانتقال إلى بكتيريا أخرى فتخيلوا .. تخيلوا .. حد
+
+110
+00:08:17,170 --> 00:08:20,590
+من زمايلكوا دارس الكتاب كله .. الكتاب ال micro
+
+111
+00:08:20,590 --> 00:08:25,930
+حافظه عن غيب .. من ايه؟ ويستطيع ان ينقل لكم كل ما
+
+112
+00:08:25,930 --> 00:08:29,670
+لديه من معلومات في ال micro biology حتى تصحوا منهم
+
+113
+00:08:29,670 --> 00:08:33,210
+تاني يوم ايه؟ تحكم micro biology صح ولا مش صح؟
+
+114
+00:08:33,790 --> 00:08:37,970
+فتخيلوا بكتيريا تنقل لكروموزوم كاملا منها إلى
+
+115
+00:08:37,970 --> 00:08:41,710
+بكتيريا أخرى، هتكسبها كل ال experience و الخبرة
+
+116
+00:08:41,710 --> 00:08:46,070
+الموجودة في معلوماتها المخزنة بالمادة الوراثية طب
+
+117
+00:08:46,070 --> 00:08:50,170
+هذا العمل كمان مرة، اقتلال، التصاد، بعدين بدينا في
+
+118
+00:08:50,170 --> 00:08:55,790
+الربع التالت انتقال كامل أو جزء لكروموزوم تمام؟
+
+119
+00:08:57,160 --> 00:09:02,700
+فصلنا هنا F بُستف و هكذا يبقى فيها ان عملية واضحة
+
+120
+00:09:02,700 --> 00:09:07,280
+انه تقسم الى نوعين النوع الاول لبلازميد فقط هي
+
+121
+00:09:07,280 --> 00:09:12,400
+اللي بتنتقل النوع التاني لبلازميد زائد دوزء او كل
+
+122
+00:09:12,400 --> 00:09:16,180
+الكروموزوم دوزء او كل الكروموزوم على حسب اجدش
+
+123
+00:09:16,180 --> 00:09:20,560
+هوفرنا وقت للانتقال تعالى نشوف الاخيرة في السلسلة
+
+124
+00:09:21,670 --> 00:09:26,950
+احنا دائما اي شيء دائري بنحاول انه نعمله للساعة
+
+125
+00:09:26,950 --> 00:09:29,770
+تعرف انا تحت ال microscope لو انت تطلع تحت ال
+
+126
+00:09:29,770 --> 00:09:33,970
+microscope و انا شوفت شغل معين بدوريكي اياها بقولي
+
+127
+00:09:33,970 --> 00:09:38,530
+تطلعي الساعة تلاتةبطلع الساعة تلاتة انت بتطلع على
+
+128
+00:09:38,530 --> 00:09:42,250
+الدائرة تبع ال microscope ال field تمام و بتتخيليك
+
+129
+00:09:42,250 --> 00:09:46,990
+ساعة وين الساعة تلاتة على الجهة هاي تمام فبتخيل لو
+
+130
+00:09:46,990 --> 00:09:50,470
+قلتلك الساعة 12 هتطلع أيه فاضح لو قلتلك في المنتصف
+
+131
+00:09:50,470 --> 00:09:54,970
+لو قلتلك الساعة تنتين يبقى نقدر نحدد اتجاهات
+
+132
+00:09:54,970 --> 00:09:59,050
+بموضوع الساعة فالساعة اللي أمامنا هذه بتوضحلنا
+
+133
+00:09:59,050 --> 00:10:03,350
+عملية خاطة جينية لل chromosome تبقى أحد البكتيري
+
+134
+00:10:03,350 --> 00:10:09,820
+بقولنا الوقت المستقرلنقل كامل لكرومزوم من خلية إلى
+
+135
+00:10:09,820 --> 00:10:14,360
+أخرى قد يستغرق مائة دقيقة، مئات دقيقة، طبعا مائة
+
+136
+00:10:14,360 --> 00:10:19,220
+دقيقة في الطبيعة غالبا لا توفر مائة دقيقة كاملة
+
+137
+00:10:19,220 --> 00:10:23,500
+تحت ثبات كامل، لازم يصير فيه disturbances، في
+
+138
+00:10:23,500 --> 00:10:26,820
+المختبر ممكن يوفر مائة دقيقة كاملة فينتقل
+
+139
+00:10:26,820 --> 00:10:28,940
+الكرومزوم، لكن في الطبيعة غالبا ماينتقلش الكرومزوم
+
+140
+00:10:28,940 --> 00:10:32,270
+كله، انتقل أجزاء من الكرومزومفبعطينا هنا هو
+
+141
+00:10:32,270 --> 00:10:36,030
+schematic presentation لكيفية الانتقال و كيف تاخد
+
+142
+00:10:36,030 --> 00:10:39,790
+وقت، فبنضغط على فقط الجزء هذا، بدنا ننجل يعني من
+
+143
+00:10:39,790 --> 00:10:45,910
+أول الساعة، اللي هي منطقة من؟ منطقة البلازميد، إلى
+
+144
+00:10:45,910 --> 00:10:50,230
+هذا الجين، بالمقابل على الجهة التانية
+
+145
+00:10:54,580 --> 00:10:58,560
+وانتقل وانتحن في مين؟ وانتحن في كروموزوم، ففي ندي
+
+146
+00:10:58,560 --> 00:11:02,100
+combination فع الأقل البكتيريا حصلت على المعلومات
+
+147
+00:11:02,100 --> 00:11:05,260
+اللي في البلازميت وعلى جزء من مين من الكروموزوم،
+
+148
+00:11:05,260 --> 00:11:10,020
+اعتقد ان الصورة واضحة ان ال six-ply لها وظيفة
+
+149
+00:11:10,020 --> 00:11:14,860
+محددة وهي ال conjugation أو نقل الجينات اللي قلنا
+
+150
+00:11:14,860 --> 00:11:18,100
+بمتمثل إما بالبلازميت فقط لحالها في ال normal
+
+151
+00:11:18,100 --> 00:11:21,120
+conjugation أو البلازميت زي جزء من مين من
+
+152
+00:11:21,120 --> 00:11:23,340
+الكروموزوم اللي سمناها high frequency
+
+153
+00:11:23,340 --> 00:11:24,260
+recombination
+
+154
+00:11:26,590 --> 00:11:33,050
+في شغل وفتحين ال .. ال .. ال .. ال animation نرجع
+
+155
+00:11:33,050 --> 00:11:36,530
+برجوها لـ cell membrane فاكرين ال cell membrane؟
+
+156
+00:11:36,530 --> 00:11:41,730
+قلنا عنه phospholipid bilayer وهي بالفعل هي ال
+
+157
+00:11:41,730 --> 00:11:44,570
+phosphate وهي ال lipid تمام؟ وهي ال phosphate وهي
+
+158
+00:11:44,570 --> 00:11:48,650
+ال lipid وهذا عبارة عن bilayer خلّينا نشتغل الـ
+
+159
+00:11:48,650 --> 00:11:55,400
+cell membrane of a prokaryotic cellبعطينا تصور عن
+
+160
+00:11:55,400 --> 00:11:57,520
+شكل ال membrane و التركيب تبعه
+
+161
+00:12:04,210 --> 00:12:08,390
+نوقف عن اللقطة هاي فكدا لما عملنا تمثيل احنا قلنا
+
+162
+00:12:08,390 --> 00:12:12,810
+ان ال membrane هو عبارة عن بحر من الدهون تسبح بيه
+
+163
+00:12:12,810 --> 00:12:16,210
+مجموعة من مين؟ من البروتينات، ايه البروتينات؟ احد
+
+164
+00:12:16,210 --> 00:12:18,510
+البروتينات هو عبارة عن integral proteins
+
+165
+00:12:18,510 --> 00:12:21,070
+الintegral proteins اللاحظوا طبعا هي عبارة عن
+
+166
+00:12:21,070 --> 00:12:26,630
+channels يعني قنوات، القنوات المتخصصةوأحنا قلنا
+
+167
+00:12:26,630 --> 00:12:31,070
+selective permeability فبالتالي هي اللي بتكون
+
+168
+00:12:31,070 --> 00:12:34,570
+بعملية تنظيم دخول وخروج الأيانات وبتختلف عن ال
+
+169
+00:12:34,570 --> 00:12:37,650
+polys أو الـ integral proteins بحسب البكتيريا
+
+170
+00:12:37,650 --> 00:12:39,910
+وبحسب نوع ال membrane
+
+171
+00:12:47,490 --> 00:12:49,950
+طبعا هذا البيئة الخارجية و هذا البيئة اللي هو
+
+172
+00:12:49,950 --> 00:12:53,610
+الداخلية اللي هي ممثلة الـ cytoplasm باللاحظ أن
+
+173
+00:12:53,610 --> 00:12:58,350
+مافيش مركبة تدخل هناكده لأ المركبة تدخل باستئذان
+
+174
+00:12:58,350 --> 00:13:02,750
+بمعنى انه عكسناك من selective permeability وبعض
+
+175
+00:13:02,750 --> 00:13:06,550
+الأحيان ندفع فلوس عكسنا ندخل اللي بيسموه active
+
+176
+00:13:06,550 --> 00:13:09,930
+transport active transport في بعض الأشياء بالسمبل
+
+177
+00:13:09,930 --> 00:13:14,810
+diffusion ندفع فلوس أقصد الفلوس اللي في ال ATP نعم
+
+178
+00:13:14,810 --> 00:13:18,700
+هي من ال ATPإن شاء الله هنزلكوا برضه دول ال .. ال
+
+179
+00:13:18,700 --> 00:13:21,520
+.. ال animation على الموقع الفيسبوك، أنا نزلت
+
+180
+00:13:21,520 --> 00:13:25,860
+بعضهم للطلاب، في عندي كمان شغل تاني بدي أوريكوا
+
+181
+00:13:25,860 --> 00:13:27,160
+إيه، هم اللي هم ال microscopy
+
+182
+00:13:34,690 --> 00:13:39,330
+أحنا التلات متفقين على أن ال microscopes أساسيات
+
+183
+00:13:39,330 --> 00:13:43,550
+أو أساسية في تعاملنا في الميكروبات لماذا؟ لأنه
+
+184
+00:13:43,550 --> 00:13:49,450
+عندي نقطة ضعف نقطة ضعف هذه تتمثل في صغر حجم
+
+185
+00:13:49,450 --> 00:13:52,090
+الميكروبات أنا اتكلمت أن ال power of resolution
+
+186
+00:13:52,090 --> 00:13:56,970
+تبتد عن غير قادرة على تمييز نقطتين من فاصلتين أصغر
+
+187
+00:13:56,970 --> 00:14:01,010
+من حد معين والبكتيري أصغر من هذا الحد فبيستلزمنا
+
+188
+00:14:01,010 --> 00:14:06,930
+أن يكون في عنديأداء للـ magnification و طبعا أحد
+
+189
+00:14:06,930 --> 00:14:09,410
+الأدوات هذه اللي هي ال light microscope ال common
+
+190
+00:14:09,410 --> 00:14:12,630
+light microscope و بعدين اتطورنا صار فينا اللي هو
+
+191
+00:14:12,630 --> 00:14:15,330
+ال electron microscope و طبعا فيه اتطورات خدوا
+
+192
+00:14:15,330 --> 00:14:18,550
+بالكوا أعلى من ال electron microscope لإن نتكلم عن
+
+193
+00:14:18,550 --> 00:14:21,790
+ال nano technology فوصلنا مايكروسكوبات نقدر نشوف
+
+194
+00:14:21,790 --> 00:14:25,790
+فيها تقريبا ال atoms وبالتالي فينا دائما باستمرار
+
+195
+00:14:25,790 --> 00:14:31,510
+لكن عندي مشكلة أخرى غير صغر الحجمإن البكتيريا ..
+
+196
+00:14:31,510 --> 00:14:35,270
+إذا بتشوفينا اللوحة هنا في two fields، واحد غير
+
+197
+00:14:35,270 --> 00:14:39,250
+مصبوغ والثاني مصبوغ، في الغير مصبوغ البكتيريا يعني
+
+198
+00:14:39,250 --> 00:14:43,330
+بالكاد يمكن أنطها، لماذا؟ لأنها طبيعتها شفافة،
+
+199
+00:14:43,330 --> 00:14:48,430
+طبيعتها شفافةفعشان انا اخليها مرئية ان بتاع هو
+
+200
+00:14:48,430 --> 00:14:52,270
+زمان كان فيه دايما يجيبوا حاجة كوميدية ان واحد
+
+201
+00:14:52,270 --> 00:14:56,470
+ماشي و بده يدخل مكان فيلاقي نفسه موصول من مين
+
+202
+00:14:56,470 --> 00:15:01,150
+بزجاج لان الزجاج شفاف فاحنا مش قادرين اصلا نميز ان
+
+203
+00:15:01,150 --> 00:15:04,910
+فيه زجاج فكانوا بيعملوا .. بيحطوا ملصق على الزجاج
+
+204
+00:15:04,910 --> 00:15:07,830
+مثلا لإنه .. لإنه بيبين انه في شغالة في هذا المكان
+
+205
+00:15:07,830 --> 00:15:12,350
+او بيعملوا painting للزجاج فاحنا مضطرين ندهن
+
+206
+00:15:13,040 --> 00:15:16,480
+البكتيريا نصبغ البكتيريا تمام حتى انا ساطيع ان انا
+
+207
+00:15:16,480 --> 00:15:18,760
+مادها ال visualizing اذا تغلبنا على المشكلتين
+
+208
+00:15:18,760 --> 00:15:23,060
+المشكلة الصغيرة الحجم والمشكلة التانية اللي هي ال
+
+209
+00:15:23,060 --> 00:15:25,740
+transparency او الشفافية الأولى بال microscopes
+
+210
+00:15:25,740 --> 00:15:29,520
+والتانية بال styling وال styling انا تحدثت او
+
+211
+00:15:29,520 --> 00:15:33,860
+طرقتله فقط لغرض واحد اوضح الفرق بين ال silvota وال
+
+212
+00:15:33,860 --> 00:15:36,600
+gram-positive وال gram-negative لكن اليوم خلينا
+
+213
+00:15:36,600 --> 00:15:42,270
+نطرقلهلو احنا يعني دخلنا فيه او اقتربنا من الدخول
+
+214
+00:15:42,270 --> 00:15:45,710
+في هذا المجال و في المعمل بالذات اول معاملة
+
+215
+00:15:45,710 --> 00:15:48,010
+هتاخدوها ان شاء الله حد هتكون عن بليستانيا
+
+216
+00:15:51,550 --> 00:15:54,650
+هذه ال microscopes في مقارنة بين ال light
+
+217
+00:15:54,650 --> 00:15:59,470
+microscope و ال electron microscope تقريبا كفكرة
+
+218
+00:15:59,470 --> 00:16:04,390
+عامة ل structures واحد انه بنضرب إلى عينا تبعتنا ب
+
+219
+00:16:04,390 --> 00:16:09,770
+light او بelectrons و بعدين بنعمل magnification و
+
+220
+00:16:09,770 --> 00:16:13,470
+بعدين نستقبل الصورة لكن الأدوات هي طبعا مختلفة
+
+221
+00:16:13,470 --> 00:16:20,620
+تمام نجي لل stylingيُقسم الـ Staining في
+
+222
+00:16:20,620 --> 00:16:22,960
+الميكروبيرجي لثلاث أقسام Simple Staining,
+
+223
+00:16:23,480 --> 00:16:27,760
+Differential Staining و Special Stain تعالوا إن
+
+224
+00:16:27,760 --> 00:16:33,640
+دابل المسميات غالبا تنشأ من وصف Simple Stain لإنه
+
+225
+00:16:33,640 --> 00:16:38,640
+بيستخدم Only One Dye، Dye معناها سبعة، Dye معناها
+
+226
+00:16:38,640 --> 00:16:44,780
+سبعة تركزوا معايا بس شوية عشان ال .. ال .. ال ..
+
+227
+00:16:44,780 --> 00:16:47,720
+ال principle of staining مرتبط باللي بتقوله الآن
+
+228
+00:16:49,550 --> 00:16:53,170
+البكتيريا كأي مخلوق كأي خلية في الدنيا فيها
+
+229
+00:16:53,170 --> 00:16:56,670
+مُركّبات و المُركّبات هذه بتكون مُكوّنة من جزيئات
+
+230
+00:16:56,670 --> 00:17:00,190
+و الجزيئات من عفن من atoms و ال atoms ممكن تكون
+
+231
+00:17:00,190 --> 00:17:03,830
+الشحنة السالبة هي الغالبة أو الشحنة الموجبة هي
+
+232
+00:17:03,830 --> 00:17:08,910
+الغالبة لكن المحصلة النهائية للسالب و الموجب في
+
+233
+00:17:08,910 --> 00:17:14,350
+الخلية لصالح السالب بمعنى ان هي الشحنة العامة تبع
+
+234
+00:17:14,350 --> 00:17:19,370
+الخلية سالبةيعني عشان نصبغها لازم نحط عليها صبغة
+
+235
+00:17:19,370 --> 00:17:21,370
+إيش؟ موشبة .. موشبة .. موشبة .. موشبة .. موشبة ..
+
+236
+00:17:21,370 --> 00:17:22,530
+موشبة .. موشبة .. موشبة .. موشبة .. موشبة .. موشبة
+
+237
+00:17:22,530 --> 00:17:22,570
+.. موشبة .. موشبة .. موشبة .. موشبة .. موشبة ..
+
+238
+00:17:22,570 --> 00:17:28,630
+موشبة .. موشبة .. موشبة .. موشبة .. موشبة .. موشبة
+
+239
+00:17:28,630 --> 00:17:41,330
+.. موشبة .. موشبة .. موشبة .. موشبة .. موشبة
+
+240
+00:17:41,330 --> 00:17:48,790
+.. موشبة .. موشاللي هو الورق الخلفي إذا كنا نصبغ
+
+241
+00:17:48,790 --> 00:17:53,950
+بكتيريا سبالة حقيقية بدنا basic diet كلمة basic
+
+242
+00:17:53,950 --> 00:17:58,950
+يعني positively charged يعني الشهرة تبعته مولا
+
+243
+00:17:58,950 --> 00:18:03,390
+فالأسباب اللي مدكورة انا اقولها تحت simple stains
+
+244
+00:18:03,390 --> 00:18:10,170
+زي crystal violet وصفرانين و مثلين بلوك كلهاbasic
+
+245
+00:18:10,170 --> 00:18:14,170
+dyes تبارك كلها هي ال crystal violet والسفرانين
+
+246
+00:18:14,170 --> 00:18:18,350
+والميثالين ال blue ما يميز ال crystal violet عشان
+
+247
+00:18:18,350 --> 00:18:21,210
+المثال إنه إذا صبغنا البكتيريا بيه إيش هيكون
+
+248
+00:18:21,210 --> 00:18:25,270
+لونها؟ violet إذا السفرانين صبغنا بيه هيكون لون
+
+249
+00:18:25,270 --> 00:18:30,130
+البكتيريا أحمر يعني بمعنى آخر البكتيريا ستتخذ نفس
+
+250
+00:18:30,130 --> 00:18:33,870
+لون الصفر اللي تصبح فيها وسميها simple لإنه هنا
+
+251
+00:18:33,870 --> 00:18:38,170
+كمان مرة بس أخدم one single dye هل كونه simple
+
+252
+00:18:38,170 --> 00:18:45,030
+وبسيط؟ليس دو أهمية لأ إله أهمية طيب أنا مش كنت
+
+253
+00:18:45,030 --> 00:18:48,770
+صايم ماكنتش أتطرق للأهميات في ال gram staining و
+
+254
+00:18:48,770 --> 00:18:51,970
+في ال simple stain لكن لأ بقى أسمعت لكل واحد منهم
+
+255
+00:18:51,970 --> 00:18:56,910
+على الأقل أهمية واحدة ال simple stain في عينا
+
+256
+00:18:56,910 --> 00:19:01,410
+مرضية ممكن يقوللي أنه في أو ما في بكتيريا طب هد
+
+257
+00:19:01,410 --> 00:19:06,450
+مهمة المعلومة؟ما هي مرادية؟ يعني ايه مرادية
+
+258
+00:19:06,450 --> 00:19:09,390
+العينة؟ اذا كانت العينة .. اذا كانت العينة من ..
+
+259
+00:19:09,390 --> 00:19:11,510
+من .. من .. فاكرين العينات لما جسمناها في الإنسان؟
+
+260
+00:19:11,510 --> 00:19:14,850
+قولنا الإنسان بينقسم إلى قسمين استرايك و استرايك
+
+261
+00:19:14,850 --> 00:19:19,050
+اذا كانت العينة استرايك و لجينا فيها بكتيريا فهذا
+
+262
+00:19:19,050 --> 00:19:23,550
+مؤشر على المرض عادة و مولود بكتيريا ينفي احتمالية
+
+263
+00:19:23,550 --> 00:19:27,090
+على قاله زيه ينفي احتمالية مولود المرض بس مولود
+
+264
+00:19:27,090 --> 00:19:30,870
+البكتيريا فهذا واحد رقم اتنين اذا انا سبقت
+
+265
+00:19:30,870 --> 00:19:34,050
+البكتيريا و شوفت البكتيرياأنا أستطيع أن أقول إنها
+
+266
+00:19:34,050 --> 00:19:37,830
+بس الـLye أو الكوكسايد، يعني الشكل تبعها، يبقى
+
+267
+00:19:37,830 --> 00:19:42,050
+الـsimple stain أعطاني نستين، الأحد الآن، presence
+
+268
+00:19:42,050 --> 00:19:45,170
+or absence of bacteria، فهذه نقطة كتير مهمة، ميّرت
+
+269
+00:19:45,170 --> 00:19:49,570
+بين فيه infection ولا فيه شيء، infection، وأعطاتني
+
+270
+00:19:49,570 --> 00:19:54,260
+شكل وترتيب الخلايا في البكتيرياكمؤشر على ممكن أن
+
+271
+00:19:54,260 --> 00:19:58,820
+يكون نوع البكتيريا المسببة إذا وجدت تنتين وكوكاي
+
+272
+00:19:58,820 --> 00:20:02,540
+بقول استربت كوكاس نيمونيا على سبيل المثال يبقى في
+
+273
+00:20:02,540 --> 00:20:06,640
+عندي أهمية للsimple stain أنه بعطيني presence or
+
+274
+00:20:06,640 --> 00:20:10,140
+absence وفي نفس الوقت بعطيني شكل and arrangement
+
+275
+00:20:10,140 --> 00:20:16,100
+نيجي لـ differential stain اسمع على النقطة هذه
+
+276
+00:20:16,100 --> 00:20:22,370
+عشان نعرف الفكرة اللي بنحكيها هي نقطةبتوضح فكرة ال
+
+277
+00:20:22,370 --> 00:20:26,290
+.. ال .. ال .. ال differentialistين واتنين اشتغلوا
+
+278
+00:20:26,290 --> 00:20:31,450
+بحصانين فأخوه فاتفق و جاله بينا نميزهم مع بعض بينا
+
+279
+00:20:31,450 --> 00:20:35,110
+نميزهم مع بعض جاله انت قص ال .. ال .. الايد اليمين
+
+280
+00:20:35,110 --> 00:20:38,710
+و انا بقص الايد الشمال للحصان فالوان طلع من ال
+
+281
+00:20:38,710 --> 00:20:41,050
+stop اللي كل واحد جاله انا جالساته يميني و انت
+
+282
+00:20:41,050 --> 00:20:43,470
+جالساته شمالك اتجاه يمينك و يميني واحد احنا جالسين
+
+283
+00:20:43,470 --> 00:20:46,930
+نفس الاشي قص رجله الشمال و رجله اليمين ايه المهم
+
+284
+00:20:46,930 --> 00:20:51,100
+في الآخر الحصانين جاعدوا بين الرجلينفنظروا لبعض
+
+285
+00:20:51,100 --> 00:20:55,460
+بعد المحاولة فقال ياسيدي بسيطة هو ما هو حصانك أسوأ
+
+286
+00:20:55,460 --> 00:21:04,180
+بدون حصاني أبيض يعني اللطيف في الموضوع إيش هو؟ إنه
+
+287
+00:21:04,180 --> 00:21:07,460
+هل نحن أصلا بحاجة إلى تمييز بين اتنين مميزين عن
+
+288
+00:21:07,460 --> 00:21:11,680
+بعض؟يبجى كان ماكانش فيه .. وهذا انا هأخدوا بالكم
+
+289
+00:21:11,680 --> 00:21:16,540
+.. إلها أساس علمي عندنا دقيق جدا ممنوع انحاول ..
+
+290
+00:21:16,540 --> 00:21:19,500
+ممنوع حتى انحاول أن نميز بين الأثنين أصلا هم
+
+291
+00:21:19,500 --> 00:21:22,880
+مختلفين يعني .. يعني خلانا نقول ذكر وأنثى، هالأنثى
+
+292
+00:21:22,880 --> 00:21:25,880
+هقول هذا صفاته كده، وهذا صفاته كده، هم مميزين
+
+293
+00:21:25,880 --> 00:21:29,640
+أساساً في البكتيريا لما تكون البكتيريا gram
+
+294
+00:21:29,640 --> 00:21:33,620
+positive أو gram negative ويتشابه في صفات مختلفة،
+
+295
+00:21:33,620 --> 00:21:37,820
+مثلانقول البكتيريا هذه اسمها staphylococcus على
+
+296
+00:21:37,820 --> 00:21:41,620
+سبيل المثال إشليكوز فيرمنتا والإشريشيا كولاي اللي
+
+297
+00:21:41,620 --> 00:21:46,880
+هي .. اللي هي gram negative اه إشليكوز فيرمنتا، هل
+
+298
+00:21:46,880 --> 00:21:50,760
+هناك ده أنه نقول كيف بنميز بين التنتة؟ طب ماهي دي
+
+299
+00:21:50,760 --> 00:21:55,430
+gram قصدي، ماهي دي gram؟وصل الصورة فعملية ال
+
+300
+00:21:55,430 --> 00:22:01,070
+differentiation هي عملية أساسية في ال microbiology
+
+301
+00:22:01,070 --> 00:22:05,850
+وبالتالي يجب علينا أن نقوم بكل ما هو ممكن لتمييز
+
+302
+00:22:05,850 --> 00:22:08,630
+بين أنواع المتشابه من الميكروبات أنا قلت
+
+303
+00:22:08,630 --> 00:22:12,130
+Salmonella و قلت Shigella مياس منهم من صفة
+
+304
+00:22:12,130 --> 00:22:17,010
+الموتليل الآن نجل لجرامستين الجرامستين تقريبا قسم
+
+305
+00:22:17,010 --> 00:22:23,200
+البكتيريا في العالم إلى قسمين جرام مصدر و جرامزي
+
+306
+00:22:23,200 --> 00:22:25,900
+بالضبط في الجامعة الإسلامية لما احنا دي نسجل في
+
+307
+00:22:25,900 --> 00:22:30,860
+الجامعة أخدت رقم انت 2013، مظبوط؟ لو أخوك سجل،
+
+308
+00:22:30,860 --> 00:22:37,740
+بياخد 2013، ليش 2013 و 2013؟ الحاسوب بمجرد أن أحط
+
+309
+00:22:37,740 --> 00:22:42,180
+له رقم واحد، بيبطل يدور في قاعة البيرتبات،
+
+310
+00:22:42,180 --> 00:22:46,680
+الطالبات بيروح على الطلاب، فعملية الاستثناء موجودة
+
+311
+00:22:46,680 --> 00:22:51,070
+حتى في عملية تصنيف الكتبفي عملية تصنيف الطلبة إلى
+
+312
+00:22:51,070 --> 00:22:56,750
+مستويات إلى .. إلى تخصصات فمهم جدا ل gram stain و
+
+313
+00:22:56,750 --> 00:23:00,390
+خلنا ناخد كيف ال gram stain بينشغل ك animation
+
+314
+00:23:00,390 --> 00:23:04,570
+يقلت ان نركز الصورة على ال .. ال .. ال bacteria
+
+315
+00:23:04,570 --> 00:23:08,570
+الآن ابتدينا نصبغهم ب crystal violet ابتدينا نصرهم
+
+316
+00:23:08,570 --> 00:23:14,750
+violetعملنا ايودين و ايودين مورده المثبت لللون
+
+317
+00:23:14,750 --> 00:23:20,370
+تشوفوا ان اللون غمك عملنا عمية غسيل بالماء تلاحظوا
+
+318
+00:23:20,370 --> 00:23:23,710
+اللون تننفيرت اضفنا الان الديكوليرايزر اللي هو
+
+319
+00:23:23,710 --> 00:23:26,610
+الكحول الان ال .. ال .. ال .. ال gram negative
+
+320
+00:23:26,610 --> 00:23:28,930
+فقلت اللون بقينا ال gram positive محتوى اللون
+
+321
+00:23:28,930 --> 00:23:34,670
+صبرنا بسافرانين تمام؟ الان السافرانين ايش صار في
+
+322
+00:23:34,670 --> 00:23:41,700
+انهانة؟ violetبعد ما حصلنا بيقولنا violet وهي
+
+323
+00:23:41,700 --> 00:23:45,160
+اللون التاني لون أحمر إذا ال gram negative و gram
+
+324
+00:23:45,160 --> 00:23:48,900
+positive اللي قال ال gram negative ل gram negative
+
+325
+00:23:48,900 --> 00:23:52,820
+بس اللي بيقولنا عنها pink و السبب في ذلك أنها فقدت
+
+326
+00:23:52,820 --> 00:23:57,940
+اللون بعد عملية اللي هو ال deodorizer طيب ماذا قام
+
+327
+00:23:57,940 --> 00:24:04,300
+ال gramstein بفعله؟ قام بإنه ميز البكتيريا
+
+328
+00:24:07,390 --> 00:24:10,650
+طيب نفس الوقت اللي ميز احنا شوفنا تحت ال
+
+329
+00:24:10,650 --> 00:24:12,270
+microscope شوفنا البكتير
+
+330
+00:24:15,060 --> 00:24:18,740
+شوفناها هل هي Bacillus ولا هي Coxal يعني فوائد ال
+
+331
+00:24:18,740 --> 00:24:22,380
+simple stain اللي شوفناها simple stain ووجود أو
+
+332
+00:24:22,380 --> 00:24:25,620
+عدم ولود البكتيريا برضه ال gram stain بعطينا إيها
+
+333
+00:24:25,620 --> 00:24:29,080
+الفوائد هذه بالإضافة إلى كل فوائد ال simple stain
+
+334
+00:24:29,080 --> 00:24:31,780
+أعطانا قدرة على التمييز إن أنا gram positive ولا
+
+335
+00:24:31,780 --> 00:24:37,520
+gram negative طبعا أعطيكوا فائدة لطيفة على أهمية
+
+336
+00:24:37,520 --> 00:24:39,780
+معرفة إن البكتيريا gram positive ولا gram negative
+
+337
+00:24:41,880 --> 00:24:46,320
+عملي في العيار بال Antibiotic معتمدة بدرجة كبيرة
+
+338
+00:24:46,320 --> 00:24:51,780
+على معرفتنا بتركيب الخلية، يعني مثلا لو جرام بوستف
+
+339
+00:24:51,780 --> 00:24:56,380
+حلاج البنسالينات والسفالوسبورينس والفانكومايوسينز
+
+340
+00:24:56,380 --> 00:24:59,100
+أنا بذكر أسماء جاهة المضادات الحيوية، هذه
+
+341
+00:24:59,100 --> 00:25:06,200
+dedicated أكثر للجرامالـ Gram Negative الـ Amino
+
+342
+00:25:06,200 --> 00:25:08,620
+Glycosides و أخرى
+
+343
+00:25:08,620 --> 00:25:27,940
+الانتيباتيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكي
+
+344
+00:25:30,140 --> 00:25:34,960
+تسموه صبغ مميز عندهم المفضل على التمييز بينما
+
+345
+00:25:34,960 --> 00:25:40,000
+الطرف التالت اسمه specialists تاني و كلمة special
+
+346
+00:25:40,000 --> 00:25:43,860
+فاكريني لما درسنا التركيط بالخلية أحد التركيط بين
+
+347
+00:25:43,860 --> 00:25:48,380
+داخلي و خارجي و كنا essential و specialو من ال
+
+348
+00:25:48,380 --> 00:25:52,520
+special غالبا مش .. الخلية يعني بتجرب تعيش من
+
+349
+00:25:52,520 --> 00:25:55,980
+غيرها من ضمن الأشياء هذه اللي هو ال capsule و ال
+
+350
+00:25:55,980 --> 00:25:59,700
+flagella ممكن ال endospore اللي شايفينه هنا تحت
+
+351
+00:25:59,700 --> 00:26:03,880
+مين ال endospore؟ تحت ال differential stain لكن هي
+
+352
+00:26:03,880 --> 00:26:07,120
+بقعها special structure و قد نطلق عليها في
+
+353
+00:26:07,120 --> 00:26:09,920
+الميكروبيرجيا أيضا كمال special لإن احنا نسبقه
+
+354
+00:26:09,920 --> 00:26:15,680
+تركيب خاص تركيب خاصطيب عشان احنا هنمرك كمان شوية
+
+355
+00:26:15,680 --> 00:26:21,500
+على ال sport فخلينا نتكلم عن ال endosport تركزوا
+
+356
+00:26:21,500 --> 00:26:27,160
+معايا شوية لأن هذا موضوع مهم جدا اسمين في ال
+
+357
+00:26:27,160 --> 00:26:30,700
+microbiology بدنا نعرفهم من الآن هم اللي يلهم
+
+358
+00:26:30,700 --> 00:26:35,120
+علاقة بهذا التركيب اللي هو ال endosport Bacillus
+
+359
+00:26:35,120 --> 00:26:37,340
+and Clostridium
+
+360
+00:26:41,910 --> 00:26:43,810
+هيموروا معانا في ال .. في ال .. في ال presentation
+
+361
+00:26:43,810 --> 00:26:48,330
+Bacillus وماروا معانا بمصطلح Bacillus Ananthracis
+
+362
+00:26:48,330 --> 00:26:52,170
+المصير بالجامعة الخبيثة وكل كما عرفته جداش هذا
+
+363
+00:26:52,170 --> 00:26:58,930
+الميكروب خطير جدا لدرجة انه هو أحد المخزونات اللي
+
+364
+00:26:58,930 --> 00:27:02,250
+موجودة في كثير من الدول اللي تمتلك فضرة على الحرب
+
+365
+00:27:02,250 --> 00:27:07,300
+البيولوجيوحرب الخليج الأولى أو التاعيلات الأولى
+
+366
+00:27:07,300 --> 00:27:13,140
+كان بحجة ان العراق تمتلك الجمرة الخبيثة ومئات
+
+367
+00:27:13,140 --> 00:27:18,300
+الأطنان او مئات الألاف من الأطنان يبقى مرض خطير او
+
+368
+00:27:18,300 --> 00:27:22,140
+مايكروب خطير ويمتلك المقدرة على تكوين الاسفول
+
+369
+00:27:22,140 --> 00:27:28,100
+بكتيريا أخرى اسمها Clostridiumكلكم سمعتم بالتتانوس
+
+370
+00:27:28,100 --> 00:27:34,320
+لما واحد بيغزه مصمع انا في غزة ست ولا سيد ومتعلم
+
+371
+00:27:34,320 --> 00:27:37,600
+مش متعلم بيقولوا اعطوه خدوه وادوه علي عيادة اعطوه
+
+372
+00:27:37,600 --> 00:27:41,700
+ابر التتانوس صح؟ هي واقع الأمر ابر ضد تتانوس ابر
+
+373
+00:27:41,700 --> 00:27:46,240
+ضد تتانوس مش ابر تتانوس الكلسترديوم التتاني هي
+
+374
+00:27:46,240 --> 00:27:50,840
+المسبب لتتانوس مايكروب مرعب مايكروب مرعب لدرجة ان
+
+375
+00:27:50,840 --> 00:27:57,140
+كل الناس خايفة منه وبتالي تحضر منهاللي هو أفراد
+
+376
+00:27:57,140 --> 00:28:01,040
+أخرين مجرمين زيه Clostridium Perifringens تسمعوا
+
+377
+00:28:01,040 --> 00:28:06,280
+بالغرغرينة؟ جسين أصابع إجليه، بعدين تكل الخدع،
+
+378
+00:28:06,280 --> 00:28:11,860
+بعدين شوية، لما خلص، و احنا جازجزش؟ الغرغرينة بسبب
+
+379
+00:28:11,860 --> 00:28:15,700
+Clostridium Perifringens طيب الميكروب تالد، برضه
+
+380
+00:28:15,700 --> 00:28:21,500
+Clostridium Clostridium Botulonium، تخيلوا؟ مجرد
+
+381
+00:28:21,500 --> 00:28:24,440
+تذوق، طعام، يحتوي على
+
+382
+00:28:51,730 --> 00:28:57,320
+استخدموه في العمليات تشبهوهاتخيلوا نفس ال toxin
+
+383
+00:28:57,320 --> 00:28:59,640
+اللي بيتكلم عنه بيستخدموه في العملية التجميلية،
+
+384
+00:28:59,640 --> 00:29:03,300
+إيش عملية تجميلية؟ هو مش عملية تجميلية، هي عملية
+
+385
+00:29:03,300 --> 00:29:08,160
+تدميرية، لكن بيسموها عملية تجميلية، لما الإنسان
+
+386
+00:29:08,160 --> 00:29:12,120
+بيكبر زينا، بيصير انه تجاعد، فالناس اللي بيحبوش
+
+387
+00:29:12,120 --> 00:29:18,070
+يكبروا، بيقولك تجاعد هذه يعني مش مناسبة لهالتجاعد
+
+388
+00:29:18,070 --> 00:29:23,450
+مانعش بتنشأ، بتنشأ غالبا من ال stress والاجهاد
+
+389
+00:29:23,450 --> 00:29:28,950
+والعضلات بتتعب وكذا، فبيحقنوا
+
+390
+00:29:28,950 --> 00:29:34,370
+المنطقة اللي فيها التجاعد بال Botox، سم مخفف جدا
+
+391
+00:29:34,370 --> 00:29:38,810
+جدا منها لأخر الجامعة، مخفف تمام؟ إيش بيعمل السم؟
+
+392
+00:29:38,810 --> 00:29:43,210
+هذا بيعمل Paralysis، شلللعضلات المنطقة اللي
+
+393
+00:29:43,210 --> 00:29:45,930
+بيحقنوا فيها، لما يصيب شرع .. يصيبوا شرع
+
+394
+00:29:45,930 --> 00:29:51,250
+الارتخائي، فبترتخي العضلات بتروح التجايد، طبعا
+
+395
+00:29:51,250 --> 00:29:55,010
+تأثيره في .. لإنه مخفف جدا، محدود في بعد ست شهور
+
+396
+00:29:55,010 --> 00:30:00,730
+بترجع التجايد مرة أخرى، لكن كثوم .. كثوم هو أقوى
+
+397
+00:30:00,730 --> 00:30:05,730
+ثوم معروف للإنسان على كوكب السبلاتيتلت مايكروبات
+
+398
+00:30:05,730 --> 00:30:09,590
+من صنف الكلوسترديوم شفتهم تيتانوس و بيرفرينجينز و
+
+399
+00:30:09,590 --> 00:30:13,250
+بوتلونيوم و مايكروب على الباسلس اللي هو الباسلس
+
+400
+00:30:13,250 --> 00:30:17,550
+أندراسيس اللي بينتج برضه توكسين و بيستخدم في الحرب
+
+401
+00:30:17,550 --> 00:30:21,330
+البيوروجية التنتين هذولة بيكونوا اندوسبورت
+
+402
+00:30:21,330 --> 00:30:24,270
+اندوسبورت اندوسبورت اندوسبورت اندوسبورت اندوسبورت
+
+403
+00:30:24,270 --> 00:30:24,610
+اندوسبورت اندوسبورت اندوسبورت اندوسبورت اندوسبورت
+
+404
+00:30:24,610 --> 00:30:26,410
+اندوسبورت اندوسبورت اندوسبورت اندوسبورت اندوسبورت
+
+405
+00:30:26,410 --> 00:30:32,350
+اندوسبورت اندوسبورت اندوسبورت
+
+406
+00:30:32,350 --> 00:30:32,670
+اند
+
+407
+00:30:41,680 --> 00:30:46,000
+المعنى تراكيب خاصة تمتلكها بعض الميكروبات لتحمي
+
+408
+00:30:46,000 --> 00:30:50,340
+نفسها من الظروف البيئية السيئة ماذا يعني بظروف
+
+409
+00:30:50,340 --> 00:30:55,330
+بيئية سيئة؟حرارة مرتفعة، حرارة منخفضة، أكسيجين
+
+410
+00:30:55,330 --> 00:30:59,210
+عالي، أكسيجين منخفض، حتى لو كان فيش nutrient، يعني
+
+411
+00:30:59,210 --> 00:31:04,070
+فيش غذاء معين للبكتيريا، بدل ما تموت من الجوع،
+
+412
+00:31:04,070 --> 00:31:10,050
+تتطوق، بتتحوصل، تتجرثم، وبتكون اندو اسفل، تمام؟
+
+413
+00:31:10,050 --> 00:31:12,910
+كمان مرة مش كل البكتيريا قادرة على هذا الفعل، only
+
+414
+00:31:12,910 --> 00:31:17,540
+two bacteriaو مادام only 2 بكتيريا يبقى مين مستهل
+
+415
+00:31:17,540 --> 00:31:20,560
+الواحد لو لدنا بكتيريا endospore-forming مميزها عن
+
+416
+00:31:20,560 --> 00:31:23,220
+برق لإن و لا البكتيريا صح ولا لأ البتالي اللي
+
+417
+00:31:23,220 --> 00:31:26,160
+استخدمنا مين endospore stain طلع على ال endospore
+
+418
+00:31:26,160 --> 00:31:29,600
+stain نصبغ
+
+419
+00:31:29,600 --> 00:31:33,680
+البكتيريا بحسب الملاكاية الجرين الملاكاية الجرين
+
+420
+00:31:33,680 --> 00:31:37,400
+لحاله ممكن تدخل لازم نسخن هي عملنا عمالية تسخين
+
+421
+00:31:37,400 --> 00:31:43,340
+عمالية heating سخننا ال sport انصبغت رسلناوضعنا
+
+422
+00:31:43,340 --> 00:31:49,940
+الآن صبغ مغير اللي هو صفرانين او Carbol Fucin بعض
+
+423
+00:31:49,940 --> 00:31:54,940
+الحالات اللي بيحطوا صفرانين حاطينها في الحالة ايش
+
+424
+00:31:54,940 --> 00:31:59,140
+صار في الخلايا بعد الغسيل ال sport صبغت باللون
+
+425
+00:31:59,140 --> 00:32:01,260
+الأخضر شايفين هذا اللون
+
+426
+00:32:04,380 --> 00:32:07,900
+إذا البكتيريا اللى فيها ال sport ال sport أخدت
+
+427
+00:32:07,900 --> 00:32:10,540
+اللون الأخضر اللون اللون أصل الملكاية ال green
+
+428
+00:32:10,540 --> 00:32:16,320
+بينما الخلية المهترقة فقدت الملكاية ال green و لما
+
+429
+00:32:16,320 --> 00:32:20,360
+صبغت مرة أخرى أخدت الصفرانين فالقاعد الخلية اللى
+
+430
+00:32:20,360 --> 00:32:24,600
+هو مصبوغة بلون أحمر و ال sport مش مصبوغة باللون
+
+431
+00:32:24,600 --> 00:32:27,020
+الأخضر لاحظوا الخلية اللى أصلا مش مكونة in the
+
+432
+00:32:27,020 --> 00:32:30,220
+sport مش فيها لون أخضر لمرة بينما اللى مكونة in
+
+433
+00:32:30,220 --> 00:32:35,580
+the sport مش فيها لون أخرفاستطعنا ان نميز الاختصار
+
+434
+00:32:35,580 --> 00:32:40,060
+بين sport former و non sport former قبل ان تكون
+
+435
+00:32:40,060 --> 00:32:47,080
+الصورة واضحة بالنسبة للاختصارات الأخرى مثل كبسول
+
+436
+00:33:15,350 --> 00:33:19,770
+لحظوا فى النهاية التنين صبغوا البكتيريا انصبغت فى
+
+437
+00:33:19,770 --> 00:33:24,210
+التنين تمام لأنه بدأنا ب basic stain بدأنا ب basic
+
+438
+00:33:24,210 --> 00:33:29,310
+stain بعدين لما صبغنا الخلفية بال أيوزين اللى
+
+439
+00:33:29,310 --> 00:33:34,030
+شايفينه قدامكم هالهالمنطقة اللى حوالين الخلايا
+
+440
+00:33:34,030 --> 00:33:37,110
+المكونة للكابسول لم تصبح لأن في أول مرة هو
+
+441
+00:33:37,110 --> 00:33:41,770
+negative stain فصبغ الخلفية بس لم يصبح اللى هو
+
+442
+00:33:41,770 --> 00:33:45,670
+الكابسول وبتالي صار فى فراغ بين الخلية البكتيريا
+
+443
+00:33:45,670 --> 00:33:49,510
+وبين الصبغ المغاير اللى هو ال background وبتالي
+
+444
+00:33:49,510 --> 00:33:53,610
+صار فى فراغ اللى شايفينه بهذا الشكل شايفينه الأبيض
+
+445
+00:33:57,810 --> 00:34:01,810
+الان .. الان .. هذا سمى special لأنه بي .. بي ..
+
+446
+00:34:01,810 --> 00:34:07,250
+بي .. بيستخدم لصباغة تراكيب خاصة لما ال endospore
+
+447
+00:34:07,250 --> 00:34:10,290
+كمان تراكيب خاص وبالتالي بالفعل في بعض الكتب
+
+448
+00:34:10,290 --> 00:34:13,390
+الميكروفالية باللاجئ التصنيف ال endospore برضه تحت
+
+449
+00:34:13,390 --> 00:34:16,910
+مين؟ تحت اللي هو special state نيجي للflagellar
+
+450
+00:34:16,910 --> 00:34:20,970
+state ال
+
+451
+00:34:20,970 --> 00:34:26,310
+Protase vulgaris المفروض ان كل التنين المفروض انهم
+
+452
+00:34:26,310 --> 00:34:32,040
+..متحركين في خطأ كبير يبدو خلينا نشوف نهاية ال
+
+453
+00:34:32,040 --> 00:34:37,460
+animation كلمة multistep flagella restaining يعني
+
+454
+00:34:37,460 --> 00:34:42,780
+الخطوة تكرر مرارا و تكرارا ليش مرارا و تكرارا؟
+
+455
+00:34:42,780 --> 00:34:47,720
+بدنا نزيد قطر لفلاجلة لحظو ده بلا تغلقها يظهر بعض
+
+456
+00:34:47,720 --> 00:34:52,760
+الصور بمعنى انه صبغنا مرارا و تكرارا و غسلنافبدنا
+
+457
+00:34:52,760 --> 00:34:57,300
+نشيل الصبغة عن البكتيريا بس بتضلها مترس برمان على
+
+458
+00:34:57,300 --> 00:35:02,140
+الفلاجللة كل مرة بنصبغ بنزيد قطر القليلة حتى يظهر
+
+459
+00:35:02,140 --> 00:35:06,440
+بهذا الشكل و زي ما توقعت أنه تنتين هما بكتيريا
+
+460
+00:35:06,440 --> 00:35:10,260
+متحرك عليهم الفلاجللة و التصنيف تبعهم إيش هنا
+
+461
+00:35:10,260 --> 00:35:19,240
+polar ولا mono ولا peri ولا .. ما اسمه؟بيري في
+
+462
+00:35:19,240 --> 00:35:22,640
+بيري تريكاس بيري تريكاس player
+
+463
+00:35:33,270 --> 00:35:36,790
+عودة الى ال presentation تبعتنا و اتكلمنا عن احنا
+
+464
+00:35:36,790 --> 00:35:41,150
+ال two functions ده ال pili و هنا ال pili ال اولى
+
+465
+00:35:41,150 --> 00:35:46,050
+ال common pili او الفمبريا موظفتها
+
+466
+00:35:46,050 --> 00:35:50,250
+الأساسية اللى هو ال adhesion او ال attachment أما
+
+467
+00:35:50,250 --> 00:35:54,350
+الثانية فكانت عبارة عن اللى هو ال contribution و
+
+468
+00:35:54,350 --> 00:35:57,130
+شرحنا ال six pili و ال common pili بس ال common
+
+469
+00:35:57,130 --> 00:36:00,190
+pili فى مثال عليها لازم نقوله فى بكتيريا اسمها
+
+470
+00:36:00,190 --> 00:36:02,390
+Neisseria gonorrhea Neisseria gonorrhea
+
+471
+00:36:03,140 --> 00:36:07,940
+السيريجونورية هي عبارة عن STD STD يعني sexually
+
+472
+00:36:07,940 --> 00:36:14,380
+transmitted bacteria هنتقل عن طريق الجنس وهي ثاني
+
+473
+00:36:14,380 --> 00:36:20,420
+أكبر مسبب ل ال STD في أوروبا وأمريكا بعد
+
+474
+00:36:20,420 --> 00:36:26,720
+الكلاميديا تتميز هذه البكتيريا بمقدرتها المهولة
+
+475
+00:36:26,720 --> 00:36:29,680
+على ال attachment عند المقدر عليها على
+
+476
+00:36:29,680 --> 00:36:34,890
+الattachment على الأسطربتوريكم الأن نقطة كتير مهمة
+
+477
+00:36:34,890 --> 00:36:41,130
+لأن لو أنا يعني بنفكر بطريق المنطق أفضل وسيلة
+
+478
+00:36:41,130 --> 00:36:46,830
+نمنعها أن نكون أجسام مضادة ضد مين؟ ضد ال بالعي
+
+479
+00:36:46,830 --> 00:36:51,430
+فبالتالي نخلي الأجسام مضادة بترتبط بال بالعي ففي
+
+480
+00:36:51,430 --> 00:36:53,610
+هذه الحالة ال بالعي بتعتقد أنها بترتبط مين؟ في
+
+481
+00:36:53,610 --> 00:36:58,250
+الأسطح عنا فبس إن أنا بعمل هيك بيكون أجسام مضادة
+
+482
+00:36:58,250 --> 00:37:03,880
+ضد ال بالعي إيش بتعمل هي بالمقابل؟بتغير من
+
+483
+00:37:03,880 --> 00:37:08,720
+أنتجينات البيلائي، فاكرين الكلام اللي حكيناه على
+
+484
+00:37:08,720 --> 00:37:13,570
+مقدرة البكتيلة على ال antigenic variation؟هذه
+
+485
+00:37:13,570 --> 00:37:16,930
+البكتيريا أحد أهم أسلحتها ال attachment، بدون
+
+486
+00:37:16,930 --> 00:37:20,190
+attachment تفقد القدرة، فلما شاهد إن الإنسان
+
+487
+00:37:20,190 --> 00:37:24,370
+بيكوّنلها أجسام مضادة ضد ال attachment، هي صارت
+
+488
+00:37:24,370 --> 00:37:27,110
+تغير من قدرتها على .. أو من ال .. من ال .. من ال
+
+489
+00:37:27,110 --> 00:37:30,670
+بالاي نفسها، عن جنية تبعيها، وبالتالي يصاب بها
+
+490
+00:37:30,670 --> 00:37:34,770
+الإنسان مرارا وتكرار، مرارا وتكرار، طبعا الناس
+
+491
+00:37:34,770 --> 00:37:40,540
+اللي بيرتكبوا الفحشةال Endospore هو تركيب من
+
+492
+00:37:40,540 --> 00:37:44,560
+التراكيب المهم جدا و حاولنا نشرح عنها قبل قليل في
+
+493
+00:37:44,560 --> 00:37:47,680
+ال studying لكن لا بأس أنه نفترق المعلومات
+
+494
+00:37:47,680 --> 00:37:51,720
+الموجودة في اللوحة Endospores are highly resistant
+
+495
+00:37:51,720 --> 00:37:57,040
+resting كلمة resting يعني non-active يعني مش ناشطة
+
+496
+00:37:57,040 --> 00:38:01,280
+الميتابوليزم فيها يعني تقريبا مقدار zero
+
+497
+00:38:06,210 --> 00:38:11,290
+Produced within cells اللي بتنتجها الخلايا They
+
+498
+00:38:11,290 --> 00:38:14,690
+are common to organisms which live in soil and may
+
+499
+00:38:14,690 --> 00:38:18,350
+need to wait out some rough time such as ارتفاع
+
+500
+00:38:18,350 --> 00:38:22,310
+درجة الحرارة, radiation, drying chemical agents
+
+501
+00:38:22,310 --> 00:38:28,530
+تحت ظروف ملائمة a spore will germinate خدوا بالكم
+
+502
+00:38:28,530 --> 00:38:34,610
+من المسمياتالخلية البكتيرية اللى بتتغدى و بتتكاتر
+
+503
+00:38:34,610 --> 00:38:38,470
+بيسموها Vegetative
+
+504
+00:38:38,470 --> 00:38:43,810
+Cell عندما تتحول الى Spore بنسمي العملية
+
+505
+00:38:43,810 --> 00:38:44,730
+Sporulation
+
+506
+00:38:47,350 --> 00:38:52,290
+عندما تتحول الـ spore إلى خلية vegetative بنسميها
+
+507
+00:38:52,290 --> 00:38:55,810
+germination عارفين ال germination بيطلق على مين؟
+
+508
+00:38:55,810 --> 00:38:59,810
+على النباتات البذور لما تنبت بنقول عنها germinate
+
+509
+00:38:59,810 --> 00:39:05,490
+فبالتالي هي تشبه العملية اللي هو الإنبات لأنه بذرة
+
+510
+00:39:05,490 --> 00:39:14,940
+تكون نبتة بذرة تكون خلية كمان مرة الخلية تسمىاللي
+
+511
+00:39:14,940 --> 00:39:20,080
+بتكاتر بتتغدى الـ vegetative cell عندما تتحول إلى
+
+512
+00:39:20,080 --> 00:39:24,320
+spore بنسمي العملية Sporulation وعندما تتحول ال
+
+513
+00:39:24,320 --> 00:39:27,020
+spore إلى خلية عادية اللي هي vegetative cell
+
+514
+00:39:27,020 --> 00:39:32,400
+بنسميها Germination عارفين
+
+515
+00:39:32,400 --> 00:39:38,720
+أنه في الكهرمان بيحتفظ بحشرات من مئات البلايين من
+
+516
+00:39:38,720 --> 00:39:42,120
+السنينبعض العلماء جالوا و طبعا الحشرات هذي ممكن
+
+517
+00:39:42,120 --> 00:39:48,060
+يكون فيها spores تعالوا نفتح الكهرمان هذا و نشوف
+
+518
+00:39:48,060 --> 00:39:51,260
+الحشرة اللي محفوظة بلايين السن أو مئات الألاف
+
+519
+00:39:51,260 --> 00:39:55,880
+السنين و نشوف إذا كان بيطلع منها بكتيريا و في يعني
+
+520
+00:39:55,880 --> 00:39:59,160
+كانت تقارير بيقول إنه استطاع العلماء أن يستنبطوا
+
+521
+00:39:59,160 --> 00:40:02,860
+الجراتيم اللي كانت محفوظة من بلايين السنين و هناك
+
+522
+00:40:02,860 --> 00:40:06,800
+أيضا كمان أخبار بيقول إن الفراعنة برضه في جدتهم
+
+523
+00:40:06,800 --> 00:40:11,790
+لقوا spores و استنبطوها مرة أخرىمعنى الحكي ان ال
+
+524
+00:40:11,790 --> 00:40:16,990
+sports قادرة على ال survival الى سنوات عديدة تقتصر
+
+525
+00:40:16,990 --> 00:40:23,390
+الى مئات الألاف من السنة ال space اللى قولنا ال
+
+526
+00:40:23,390 --> 00:40:26,390
+genesis التانين اللى هم البصيلاس و ال clostridium
+
+527
+00:40:26,390 --> 00:40:29,770
+في الآخر اللوحة اللى قدامنا الآن الصورة اللى
+
+528
+00:40:29,770 --> 00:40:34,010
+أمامكم بتوضح ال location of ال sport location
+
+529
+00:40:34,010 --> 00:40:39,550
+locationاحنا اتفقنا انه فيه او مافيه sport صح؟ و
+
+530
+00:40:39,550 --> 00:40:43,210
+بس تنتهي اللي عندنا وكليسترديوم و بصلص، هي مبتنية
+
+531
+00:40:43,210 --> 00:40:48,110
+على ال sport يعني، تمام؟الان الشكل اللى أمامكوا
+
+532
+00:40:48,110 --> 00:40:52,370
+بيوضح لاحظوا ال sport هنا و اللى موجودة في المنتصف
+
+533
+00:40:52,370 --> 00:40:56,810
+خارج الحدود و لا دمونه حدود الخلية طب هي في
+
+534
+00:40:56,810 --> 00:41:02,010
+المنتصف لكن عاملة bulging خارج حدود الخلية في
+
+535
+00:41:02,010 --> 00:41:04,690
+اختلاف ولا مش اختلاف اذا بتكلمنا عن ال location
+
+536
+00:41:04,690 --> 00:41:09,130
+central انا عندي terminal طب هنا terminal هنا
+
+537
+00:41:09,130 --> 00:41:15,190
+terminal هنا terminal ovalوهي ال terminal هو مش
+
+538
+00:41:15,190 --> 00:41:23,810
+circle لإنها كروي لإنها كروي لكن صار عندي location
+
+539
+00:41:23,810 --> 00:41:29,590
+صار عندي morphology شكل يعني بوضحلي أنها لي oval
+
+540
+00:41:29,590 --> 00:41:33,070
+ولا spherical هل هي within the boundaries of the
+
+541
+00:41:33,070 --> 00:41:37,630
+cell ولا bulging يعني خارج أطاق ال cells طيب هذا
+
+542
+00:41:37,630 --> 00:41:41,980
+المعلومات بتفيدنا ولا متفادنها ايش؟بتفيدنا في .. و
+
+543
+00:41:41,980 --> 00:41:44,860
+هذا الكلام لما ندرس ال diagnostic microbiology،
+
+544
+00:41:44,860 --> 00:41:48,140
+هندرس مثلا و نقولكوا Bacillus anthracis، هنقولكوا
+
+545
+00:41:48,140 --> 00:41:51,480
+ان ال sport في ال Bacillus anthracis is centrally
+
+546
+00:41:51,480 --> 00:41:54,960
+located، هذي بطلت معلومة على الهامش، صارت معلومة
+
+547
+00:41:54,960 --> 00:41:58,620
+ضمن المعلومات المستخدمة في عملية التعرف على
+
+548
+00:41:58,620 --> 00:42:02,380
+البكتيريا نفسها، أرجوا الانتباه إلى هذه القضية
+
+549
+00:42:05,650 --> 00:42:16,690
+فيه انحاسمة Dipicronic Acid او باقتصاروه DAP ال
+
+550
+00:42:16,690 --> 00:42:21,730
+Dipicronic Acid موجود
+
+551
+00:42:21,730 --> 00:42:30,610
+في البكتيريا بكميات نادرة جدا يعني كميات قليلة جدا
+
+552
+00:42:30,610 --> 00:42:38,280
+لكن في ال sportبعشرات المرات من تركيزه في وين؟ في
+
+553
+00:42:38,280 --> 00:42:43,740
+الخلية العادية لأنه ارتبط بال sport كميته كتر في
+
+554
+00:42:43,740 --> 00:42:46,680
+ال sport فهناك اعتقاد انه
+
+555
+00:42:50,940 --> 00:42:56,640
+وهو المسئول عن توفير ال protection للبكتيريا بشكل
+
+556
+00:42:56,640 --> 00:42:59,400
+كبير Spores that were dormant for thousands of
+
+557
+00:42:59,400 --> 00:43:03,600
+years in the great tombs of the Egyptian pharaohs
+
+558
+00:43:03,600 --> 00:43:08,320
+الذين كانوا قادرين على ترميلها و تنفجرها عندما
+
+559
+00:43:08,320 --> 00:43:12,780
+كانوا موضعينها في مصطلحات مناسبة هنا مرة أخرى
+
+560
+00:43:12,780 --> 00:43:16,780
+There are even claims هناك دعائات
+
+561
+00:43:20,130 --> 00:43:25,010
+over 250 million years old being able to generate
+
+562
+00:43:25,010 --> 00:43:28,370
+when placed in appropriate means نفس المعلومة اللي
+
+563
+00:43:28,370 --> 00:43:33,530
+ذكرت أنه جراثيم أمرها أكتر من 250 مليون سنة، هناك
+
+564
+00:43:33,530 --> 00:43:39,080
+إدعاءات اللي تم استنباطها لموضوع مرة أخرىالجملة
+
+565
+00:43:39,080 --> 00:43:42,840
+الأخيرة كون احنا بنا أدمين علميين بنقول these
+
+566
+00:43:42,840 --> 00:43:46,700
+results have yet to be validated هذه النتائج يجب
+
+567
+00:43:46,700 --> 00:43:55,900
+أن يتحقق منها يعني مش معلومات مؤكدة مسلمة طيب
+
+568
+00:43:55,900 --> 00:43:59,340
+بنتحكلكوا بس يعني كيف بتكون ال sports او طريقة
+
+569
+00:43:59,340 --> 00:44:01,500
+تكوين ال sport عشان برضه تكون الصورة واضحة عندي
+
+570
+00:44:03,450 --> 00:44:07,110
+هنسمع كلمة sport في مكانين مختلفين، في البكتيريا
+
+571
+00:44:07,110 --> 00:44:12,530
+وفي الفطريات المفهوم لل sport في البكتيريا يختلف
+
+572
+00:44:12,530 --> 00:44:17,070
+تماما عن ال sport في الفطريات ال sport في الفطريات
+
+573
+00:44:17,070 --> 00:44:23,130
+هي أداة من أدوات تكاتب، الخلية الواحدة بتنتج
+
+574
+00:44:23,130 --> 00:44:28,170
+ملايين ال sportملايين lesbois اعتقد كلكوا في
+
+575
+00:44:28,170 --> 00:44:34,190
+المطبخ عفا عندكم إشي حبة برتقال أو فماطن أو حتى
+
+576
+00:44:34,190 --> 00:44:38,590
+خبز وبتلاقوا عليه عفا أطرافه وبتكون بيضاء اللون
+
+577
+00:44:38,590 --> 00:44:43,570
+وفي المتصفر بيكون أسود أو أخضر أو أزرق أو أصفر لو
+
+578
+00:44:43,570 --> 00:44:47,810
+طلعتوا فنجلة من القهوة على المجلة لمدة مثلا أسبوع
+
+579
+00:44:47,810 --> 00:44:50,670
+أو أسبوعين طلعوا عليها هتلاقوا بالفعل cottony
+
+580
+00:44:50,670 --> 00:44:53,910
+white وفي المتصفر فيه انها greenish discolored لون
+
+581
+00:44:53,910 --> 00:45:00,710
+أخضرهذا عبارة عن fungus mold هذا ال mold غالبا
+
+582
+00:45:00,710 --> 00:45:04,590
+بيكون له أبيض cottony white لكن اللون اللي انتوا
+
+583
+00:45:04,590 --> 00:45:08,170
+شايفينه الأخضر أو الأسود أو الأزرق أو الأصفر هو
+
+584
+00:45:08,170 --> 00:45:12,790
+لون ال spores اللي لو هذا اللي في الفنجان الصغير
+
+585
+00:45:12,790 --> 00:45:17,500
+أخدناه من عندهم بيلاقيهم بلايين ال sporesتستخدم
+
+586
+00:45:17,500 --> 00:45:21,580
+للتكاثر، تستخدم لا للتكاثر وليس للحماية، بينما
+
+587
+00:45:21,580 --> 00:45:26,440
+البكتيريا تستخدم لمين؟ لل protection، ما بتستخدمش
+
+588
+00:45:26,440 --> 00:45:29,540
+لتكاثر، خلية واحدة تتجرث بيصير one spore، ال one
+
+589
+00:45:29,540 --> 00:45:35,040
+spore بيصير germination، تمام؟ بترجع خلية واحدة،
+
+590
+00:45:35,040 --> 00:45:38,360
+فخلية، spore، خلية، مش تكاثر، مافيش تكاثر
+
+591
+00:45:42,680 --> 00:45:46,000
+هي عبارة عن multiple membranes، لاحظوا السماكة
+
+592
+00:45:46,000 --> 00:45:49,360
+تبعتها، هاي ال cell wall، تمام، هاي ال cell wall
+
+593
+00:45:49,360 --> 00:45:53,260
+تبع المادة العالية، وهي ال cell wall تبع ال
+
+594
+00:45:53,260 --> 00:45:58,300
+sports، طلعوا أجدش السماكة تبعته، بتعرفوا الجربان،
+
+595
+00:45:58,300 --> 00:46:01,940
+كيف بتضبوه في الخزانة؟مش بتحطوه و بتجلبوه و
+
+596
+00:46:01,940 --> 00:46:06,020
+بترجعوه نفس الفكرة بيتكوّن ل sports بيسموها
+
+597
+00:46:06,020 --> 00:46:09,320
+phagocytase phagocyte هذي بتاكل هذي و هذي بتاكل
+
+598
+00:46:09,320 --> 00:46:13,100
+هذي بمعنى أخذ ال cell membrane بيصير يكوّن طبقات و
+
+599
+00:46:13,100 --> 00:46:17,860
+بيراكم بيبتدوا جليكان و بيراكم D-baconic acid D
+
+600
+00:46:17,860 --> 00:46:23,850
+-baconic acidفبصير عندي تركيب ذو سماكة عالية و
+
+601
+00:46:23,850 --> 00:46:27,090
+تركيب خاص من ال cell wall قادر على منع دخول
+
+602
+00:46:27,090 --> 00:46:30,870
+الرطوبة و داخل دخول ال chemicals حتى ال radiation
+
+603
+00:46:30,870 --> 00:46:34,150
+ال ultraviolet light اللي بتقتل البكتيريا العالية
+
+604
+00:46:34,150 --> 00:46:38,350
+غير قادر على قتل البكتيريا اللي لها indoor asthma
+
+605
+00:46:38,350 --> 00:46:46,410
+شايفين الجداش في عندي تركيب داخل اللي هوالـ spores
+
+606
+00:46:46,410 --> 00:46:50,750
+هذه الخلية وهي الـ spores تبقى تحتها هذه اللي
+
+607
+00:46:50,750 --> 00:46:54,690
+شايفينها في جدامكوا هذه هذا الشكل ال typical
+
+608
+00:46:54,690 --> 00:47:02,570
+النموذجي لكلسترديوم تيتانيه عرض المصماري هذا
+
+609
+00:47:02,570 --> 00:47:06,810
+الخلية وهي
+
+610
+00:47:06,810 --> 00:47:10,510
+ال spore ال spore وين جاي على الطرف بيسموها
+
+611
+00:47:10,510 --> 00:47:15,670
+terminal مش فيه كانت هنا central احنايوجد على
+
+612
+00:47:15,670 --> 00:47:19,310
+الطرف بيسموها subterminal وعلى الطرف بيسموها
+
+613
+00:47:19,310 --> 00:47:20,010
+terminal
+
+614
+00:47:24,550 --> 00:47:26,790
+ال microscope احنا اظن اننا مارين عليهم في ال
+
+615
+00:47:26,790 --> 00:47:30,030
+animation مش هنتكلم عن طريقهم كل اللي .. اللي ..
+
+616
+00:47:30,030 --> 00:47:32,850
+اللي .. اللي انذكر الان هنا simple staining ال
+
+617
+00:47:32,850 --> 00:47:35,070
+differential و ال special staining كل ما دول مارين
+
+618
+00:47:35,070 --> 00:47:38,010
+عليهم فداك احنا بنكون ان هنا الجزء المتعلق بال
+
+619
+00:47:38,010 --> 00:47:40,610
+bacterial cell structure و ان شاء الله هيكون
+
+620
+00:47:40,610 --> 00:47:44,450
+المحاضرة القادمة عن ال bacterial testulary الموضوع
+
+621
+00:47:44,450 --> 00:47:47,810
+برضه interesting و ان شاء الله يكون مفيد لنا
+
+622
+00:47:47,810 --> 00:47:48,530
+السلام عليكم
+
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/uz47-1oLtNU.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/uz47-1oLtNU.srt
new file mode 100644
index 0000000000000000000000000000000000000000..1c0d9c2aa8e402e0326798d96451cc0f83b65fd3
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/uz47-1oLtNU.srt
@@ -0,0 +1,2570 @@
+1
+00:00:20,860 --> 00:00:25,120
+بسم الله الرحمن الرحيم نكمل مع بعض سويا الموضوع
+
+2
+00:00:25,120 --> 00:00:29,840
+بعنوان the biogenic coccus وكلمة biogenic coccus
+
+3
+00:00:29,840 --> 00:00:33,480
+اتفقنا على أنه بيربطها معنايا أساسيا أن البكتيريا
+
+4
+00:00:33,480 --> 00:00:37,780
+اللي بتتحدث عنها هي كوكساي كروية الشكل ومن سماتها
+
+5
+00:00:37,780 --> 00:00:40,980
+أنها بتعمل biogenic infection أو ما يسمى بال pus
+
+6
+00:00:40,980 --> 00:00:46,830
+producing infection اطّلعنا على بعض الصور اللي بتوضح
+
+7
+00:00:46,830 --> 00:00:52,690
+الأشكال المختلفة اللي هندرسها تحت هذا الموضوع شفنا
+
+8
+00:00:52,690 --> 00:00:59,370
+شكلها الرئيسيين اللي هم ال chains و ال clusters ال
+
+9
+00:00:59,370 --> 00:01:03,450
+chains و ال clusters في اللوحات اللي أمامكم
+
+10
+00:01:03,450 --> 00:01:07,670
+معظمهم لـ ال streptococci فقط الوحيدة اللي منهم
+
+11
+00:01:07,670 --> 00:01:12,830
+استثناء لـ  streptococci اللي على يمين الشاشة في الأعلى
+
+12
+00:01:18,420 --> 00:01:21,240
+وبدأنا بالحديث عن الـ Staphylococci والـ
+
+13
+00:01:21,240 --> 00:01:24,940
+Staphylococci هي المجموعة الأهم حقيقة من حيث عدد ال
+
+14
+00:01:24,940 --> 00:01:28,520
+infection دي اللي بتسببها كلمة عدد يعني بتتكلم
+
+15
+00:01:28,520 --> 00:01:32,180
+فيها frequency، frequency يعني نسبة الإصابة كم مرة
+
+16
+00:01:32,180 --> 00:01:35,700
+بيُصاب البشر بهذا ال microbe لأن هتكلم عن ال
+
+17
+00:01:35,700 --> 00:01:40,860
+Staphylococci برضه بأكثر لكن أكثر من ناحية الأنواع
+
+18
+00:01:40,860 --> 00:01:44,620
+وليس ال frequency، أنواع ال infection ال
+
+19
+00:01:44,620 --> 00:01:49,680
+Staphylococci ميزاتها كانت gram positive فكانت تبقى
+
+20
+00:01:49,680 --> 00:01:53,340
+نقلة ال arrangement بتاعتها in clusters واتفقنا أنه
+
+21
+00:01:53,340 --> 00:01:57,320
+لا نستطيع الحكم على ال clusters إلا من خلال اللي هو
+
+22
+00:01:57,320 --> 00:02:00,720
+ال catalase test إذا كانت catalase positive هيُستفاد
+
+23
+00:02:00,720 --> 00:02:04,560
+كوكاي it is catalase positive major component of the
+
+24
+00:02:04,560 --> 00:02:10,660
+nose and skin normal microbiota شوفنا أشكال لها
+
+25
+00:02:10,660 --> 00:02:16,540
+من ال pure culture و أشكال من عينات صديق شفنا بحث
+
+26
+00:02:16,540 --> 00:02:19,320
+ال catalase اللي تحدثنا عنه المرة الماضية و قلنا
+
+27
+00:02:19,320 --> 00:02:21,480
+أنه يبقى عن قطرة من hydrogen peroxide و تُضاف إلى
+
+28
+00:02:21,480 --> 00:02:24,360
+البكتيريا إذا طلع عندنا ال bubbles فهي positive إذا
+
+29
+00:02:24,360 --> 00:02:28,240
+ما طلع عندنا ال bubbles بتكون catalase negative وبدأنا
+
+30
+00:02:28,240 --> 00:02:30,380
+بالحديث اللي على الوضع الأصعب اللي هو ال
+
+31
+00:02:30,380 --> 00:02:32,420
+pathogenesis و ال immunity للـ Staphylococcus و
+
+32
+00:02:32,420 --> 00:02:35,860
+اتكلمنا عن اللي هو ال capsule، اتكلمنا عن ال
+
+33
+00:02:35,860 --> 00:02:39,440
+protein A، اتكلمنا عن ال teichoic acid، اتكلمنا عن
+
+34
+00:02:39,440 --> 00:02:44,300
+ال clumping factor أو ال coagulase substance اللي 
+
+35
+00:02:44,300 --> 00:02:48,920
+موجود في البكتيريا، وشوفنا صورة لل protein A كيف
+
+36
+00:02:48,920 --> 00:02:52,300
+بيجلب أو بيرتبط بال FC portion بتاعته اللي هو ال
+
+37
+00:02:52,300 --> 00:02:56,430
+antibody مجموعة من الـ enzymes والتوكسين برضه
+
+38
+00:02:56,430 --> 00:02:59,630
+لوحة كاملة وركزنا في آخر رزقين اللي هم الـtoxic
+
+39
+00:02:59,630 --> 00:03:03,050
+shock syndrome toxin والـ enterotoxin واتفقنا على
+
+40
+00:03:03,050 --> 00:03:06,010
+أن الاتنين super antigens لكن واحد acted
+
+41
+00:03:06,010 --> 00:03:08,670
+systematically وواحد acted locally في ال
+
+42
+00:03:08,670 --> 00:03:13,830
+intestinal tract وهذا الشكل أو الصورة وضحت لنا ال
+
+43
+00:03:13,830 --> 00:03:16,830
+mechanism by which super antigen works by joining
+
+44
+00:03:16,830 --> 00:03:21,730
+two cells اللي هم ال T cell and اللي قلنا عنها ال
+
+45
+00:03:21,730 --> 00:03:27,710
+macrophage و برضه تعرجنا على بعض الأمراض اللي اللي
+
+46
+00:03:27,710 --> 00:03:30,070
+علاقة بال skin و ال systemic و قولنا إنه في ال
+
+47
+00:03:30,070 --> 00:03:33,190
+skin فيه folliculitis و فينا boils اللي قولنا ..
+
+48
+00:03:33,190 --> 00:03:36,830
+اللي سميناها بالعربي شحاطة ال N-P-T-G و الحصف ال
+
+49
+00:03:36,830 --> 00:03:40,050
+Impetigo و ال exfoliative dermatitis و
+
+50
+00:03:40,050 --> 00:03:44,230
+تانية قولنا فيهم دتلة الخلايا بتاعة الجلد و ال
+
+51
+00:03:44,230 --> 00:03:47,470
+mastitis اللي هي تهيأة التدري أو الضرر abscess
+
+52
+00:03:47,470 --> 00:03:50,970
+اللي قولنا عنها هي deep folliculitis تمام؟ بتكون
+
+53
+00:03:50,970 --> 00:03:57,440
+موجودة في .. إنه في ال ..الكبد أو في الدماغ أو في
+
+54
+00:03:57,440 --> 00:04:02,920
+أماكن لكن اتفقنا على أن كل ال infections هذه لها
+
+55
+00:04:02,920 --> 00:04:03,140
+ال
+
+56
+00:04:06,090 --> 00:04:09,810
+ال deep tissues بتكون الإصابة جاية من wound
+
+57
+00:04:09,810 --> 00:04:12,790
+infection على سبيل المثال يعني مثلًا التهاب جروح أو
+
+58
+00:04:12,790 --> 00:04:18,170
+كأن abscess خارجي أو folliculitis ومن ثم تخرب البكتيريا
+
+59
+00:04:18,170 --> 00:04:22,230
+من مكانها الأصلي إلى الدم و بتعمل اللي هي septicemia
+
+60
+00:04:22,230 --> 00:04:27,650
+ممكن تصل إلى مكان مختلف ومتنوع شفنا الصورة هذه
+
+61
+00:04:27,650 --> 00:04:31,110
+برضه و اتكلمنا عن أن هذه ال impetigo وقلنا أن هذا
+
+62
+00:04:31,110 --> 00:04:34,230
+very contagious ويمكن أول مرة نستخدم مصطلح
+
+63
+00:04:34,230 --> 00:04:38,750
+contagious Infectious معناها مُعدٍ بينتقل من إنسان
+
+64
+00:04:38,750 --> 00:04:42,510
+إلى إنسان أو من حيوان إلى إنسان Contagious هو مُعدٍ
+
+65
+00:04:42,510 --> 00:04:45,670
+لكن بدرجة أكثر من كلمة infectious بمعنى آخر أن
+
+66
+00:04:45,670 --> 00:04:51,230
+نسبة العدوى فيه بتكون سريعة وعالية وبرضه كمان
+
+67
+00:04:51,230 --> 00:04:53,990
+اتطرقنا لموضوع مهم جدًا لما اتكلمنا عن ال food
+
+68
+00:04:53,990 --> 00:04:57,370
+poisoning وميزنا بين ال food poisoning و ال food
+
+69
+00:04:57,370 --> 00:05:02,290
+infection وأكد على أن ال food poisoning ارتبط
+
+70
+00:05:02,290 --> 00:05:09,110
+تسميته بتناول الإنسان بتناوله طعام يحتوي على توكسين
+
+71
+00:05:09,110 --> 00:05:12,370
+مش بالضرورة على الميكروب يمكن الميكروب يكون قام
+
+72
+00:05:12,370 --> 00:05:16,350
+بالتوكسين ومات لكن أنا تناولت فقط أو ال symptoms
+
+73
+00:05:16,350 --> 00:05:19,610
+اللي صارت عندنا من وين؟ من التوكسين بينما ال food
+
+74
+00:05:19,610 --> 00:05:23,250
+infection تناولنا الغذاء فيه micro organism قد
+
+75
+00:05:23,250 --> 00:05:26,230
+يكون العدد قليل لكن كافي أن يحدث عندي أنا
+
+76
+00:05:26,230 --> 00:05:30,230
+infection و growth وبعدين الميكروب هو ينتج ال
+
+77
+00:05:30,230 --> 00:05:32,970
+toxin ويعمل disease في الداخل هذا سميناه food
+
+78
+00:05:32,970 --> 00:05:36,750
+infection فبنميز بين food poisoning و food
+
+79
+00:05:36,750 --> 00:05:40,910
+infection غالبًا ال poisoning أعراضه بتبدأ سريعًا
+
+80
+00:05:40,910 --> 00:05:44,170
+بينما ال infection قد تستمر يومين تلاتة أو تبدأ
+
+81
+00:05:44,170 --> 00:05:46,490
+بعد يومين أو تلاتة حسب نوع الميكروب اللي بيتكلم
+
+82
+00:05:46,490 --> 00:05:50,820
+عنه هذه طبعًا الصورة كانت لل toxic shock syndrome
+
+83
+00:05:50,820 --> 00:05:54,080
+لما اتكلمنا عن ال tampons وقلنا أنه فيه مجموعة من
+
+84
+00:05:54,080 --> 00:05:56,940
+ال symptoms زي ال fever ال rashes و ال
+
+85
+00:05:56,940 --> 00:06:01,060
+desquamation و ال vomiting و ال diarrhea و ال
+
+86
+00:06:01,060 --> 00:06:04,120
+myalgias و أن ال myalgias اللي هي ألم العضلات
+
+87
+00:06:06,740 --> 00:06:09,560
+طبعًا هذه الصورة أيضًا أعتقد أننا أنهينا فيها
+
+88
+00:06:09,560 --> 00:06:12,260
+المحاضرة اللي اتكلمنا عنه اللي هو scalded skin
+
+89
+00:06:12,260 --> 00:06:16,600
+syndrome اللي جونا فيه توكسين اسمه exfoliatin وهذا
+
+90
+00:06:16,600 --> 00:06:19,740
+ال exfoliatin هو المسؤول عن ال peeling off لل skin
+
+91
+00:06:19,740 --> 00:06:25,200
+تبع ال babies المصابين لا أعتقد أننا وصلنا لـ ..
+
+92
+00:06:25,200 --> 00:06:29,060
+وصلنا لمرحلة والصورة اللي قولنا عنها دي الصورة
+
+93
+00:06:29,060 --> 00:06:33,160
+اللي موجودة هي تعبير عن حالة واحدة اسمها phage
+
+94
+00:06:33,160 --> 00:06:36,700
+typing يعني مش خاصة بالصفر ال Staphylococci ولكن معبرة عن
+
+95
+00:06:36,700 --> 00:06:40,120
+أي حالة اسمها phage typing والفراغات اللي احنا
+
+96
+00:06:40,120 --> 00:06:45,420
+شايفينها الصغيرة ها دي هي عبارة عن مناطق ال phage عمل
+
+97
+00:06:45,420 --> 00:06:48,660
+فيها infection وبالتالي صار فيها lysis سميناها
+
+98
+00:06:48,660 --> 00:06:55,310
+plaques أي Lysis من قبل ال phage يعني أن ال phage specific
+
+99
+00:06:55,310 --> 00:06:59,910
+للبكتيريا، إذا كان ال phage معروف والبكتيريا مجهولة
+
+100
+00:07:01,030 --> 00:07:05,310
+واتفقنا على أنه يُستخدم فيه عميق التعامل ثلاث صفات
+
+101
+00:07:05,310 --> 00:07:09,110
+للـ Staphylococcus aureus ذكرناهم المحاضرة السابقة بدنا
+
+102
+00:07:09,110 --> 00:07:13,150
+نؤكد عليهم مرة أخرى mannitol fermentation coagulase
+
+103
+00:07:13,150 --> 00:07:17,490
+test وال DNAse ثلاث صفات positive تلاتة positive
+
+104
+00:07:17,490 --> 00:07:21,330
+بيُميزوا الـ Staphylococcus aureus عن باقي أنواع
+
+105
+00:07:21,330 --> 00:07:25,750
+الـ Staphylococcus أحدكم بيسأل بيقول أنه طيب بكفي ال
+
+106
+00:07:25,750 --> 00:07:29,290
+coagulase اللي أخذته في المعمل هو ال Staphylococci مميز من
+
+107
+00:07:29,290 --> 00:07:33,350
+الـ Staphylococcus أول سباق الـ Staphylococcus aureus
+
+108
+00:07:33,350 --> 00:07:33,690
+بتقول
+
+109
+00:07:38,460 --> 00:07:41,380
+the more tests you use the more accurate is your
+
+110
+00:07:41,380 --> 00:07:44,780
+diagnosis كل ما زاد عدد الفحوصات كل ما زاد دقة
+
+111
+00:07:44,780 --> 00:07:49,280
+التعرف لعل لو عشان نكون في microbe احنا ما بنعرفه
+
+112
+00:07:49,280 --> 00:07:52,360
+أساسًا يعني environmental microbe بيسمي ال microbe
+
+113
+00:07:52,360 --> 00:07:57,980
+بنعرفه وبالتالي لسنا بصدد هنا أن نعمل تعريف غير
+
+114
+00:07:57,980 --> 00:08:02,980
+دقيق بدنا تعريف دقيق، ركزنا عليها ولا ما ركزنا
+
+115
+00:08:02,980 --> 00:08:07,430
+عليها ال antibiotic sensitivity الـ Staphylococcus
+
+116
+00:08:07,430 --> 00:08:11,650
+aureus من الميكروبات المشهورة، مشهورة المعنى آخر
+
+117
+00:08:11,650 --> 00:08:16,310
+يعني موجودة في مصاف النجوم في عالم الـ
+
+118
+00:08:16,310 --> 00:08:19,150
+Antimicrobial resistance من أوائل الميكروبات
+
+119
+00:08:19,150 --> 00:08:23,390
+أربعينيات القرن الماضي البنسلين تم اكتشافه كان هو
+
+120
+00:08:23,390 --> 00:08:27,430
+ال magic لكل magic drug لكل Staphylococcal
+
+121
+00:08:27,430 --> 00:08:31,310
+infection بعد خمس عشرة سنة بدأنا نلاحظ resistance
+
+122
+00:08:31,310 --> 00:08:38,380
+طورنا ال penicillin وفي الآخر حصلنا على وضع مزير
+
+123
+00:08:38,380 --> 00:08:41,060
+للغاية شايفين الأسماء الأخيرة اللي موجودة على
+
+124
+00:08:41,060 --> 00:08:45,060
+اللوحة اللي هي penicillin, methicillin و vancomycin
+
+125
+00:08:45,060 --> 00:08:49,120
+اللي هنا هي penicillin resistant بنسبة 95% لـ 99%
+
+126
+00:08:49,120 --> 00:08:55,180
+في غزة عندنا هنا بنسبة من 95% إلى 99% الـ meticillin
+
+127
+00:08:55,180 --> 00:08:57,680
+وصلت الـ resistance rate  إلى أكثر من 40%
+
+128
+00:09:02,680 --> 00:09:06,680
+ببدأ ظهور الـ Vancomycin-resistant Staphylococcus 
+
+129
+00:09:06,680 --> 00:09:12,660
+aureus دراسة قبل أقل من شهر طلعت ثلاثة ونصف في المئة
+
+130
+00:09:12,660 --> 00:09:16,080
+يعني معناه إنّي أنا فيها عندي مشكلة حقيقية
+
+131
+00:09:16,080 --> 00:09:18,880
+يعني هيجي يوم من الأيام ما أستطيع أن أعالج
+
+132
+00:09:18,880 --> 00:09:21,040
+Staphylococcus aureus بالـ available drugs اللي
+
+133
+00:09:21,040 --> 00:09:24,380
+موجودة في الأسواق طبعًا الـ available drugs اللي هي
+
+134
+00:09:24,380 --> 00:09:30,560
+يمكن استخدامها استخدامًا للأدمة الصورة اللي قدامنا هي
+
+135
+00:09:30,560 --> 00:09:33,200
+دي بتوضح الـ hemolysis اللي حكينا عنه في المحاضرة
+
+136
+00:09:33,200 --> 00:09:36,920
+السابقة اللي هو beta .. beta hemolysis complete
+
+137
+00:09:36,920 --> 00:09:40,200
+hemolysis .. complete hemolysis الصورة هي بتوضح إنّ
+
+138
+00:09:40,200 --> 00:09:42,280
+الـ .. الـ .. مش الـ hemolysis تعفو عنه الـ manitol
+
+139
+00:09:42,280 --> 00:09:46,400
+salt agar هذا بده وقفة ويردنا نركز عنه شوية الـ
+
+140
+00:09:46,400 --> 00:09:50,700
+manitol salt agar هو عبارة عن selective and
+
+141
+00:09:50,700 --> 00:09:54,120
+differential كلكم بتعرفوا إيش أنا selective وإيش
+
+142
+00:09:54,120 --> 00:09:57,360
+أنا differential الآن كيف صار selective في هذا
+
+143
+00:09:57,360 --> 00:10:03,880
+الوسط؟ أضفنا له 7.5% Sodium Chloride 7.5% Sodium
+
+144
+00:10:03,880 --> 00:10:09,520
+Chloride عارفين إيش يعني 7.5%؟ يعني لما نحضر لتر
+
+145
+00:10:09,520 --> 00:10:14,980
+يعني 75 جرام ده لتر يعني الملوحة تبعتها أعلى من
+
+146
+00:10:14,980 --> 00:10:21,320
+ملوحة البحر بضعفين تقريبًا أعلى بضعف ملوحة
+
+147
+00:10:21,320 --> 00:10:25,860
+البحر مين ممكن ينمو في هذه الـ concentration من الـ
+
+148
+00:10:25,860 --> 00:10:29,260
+Sonium chloride للاستفيلو كوكاية؟ يبقى المناعة
+
+149
+00:10:29,260 --> 00:10:34,280
+تنمو الميكروبات الأخرى، مش بس الـ Stripto، حتى
+
+150
+00:10:34,280 --> 00:10:37,280
+اللي إمتلا بكترياسي المناعة تنموها لأنك لن تستطيع
+
+151
+00:10:37,280 --> 00:10:42,620
+تنمو في 7.5% يبقى الوضع تصبح selective لمين؟ 
+
+152
+00:10:42,620 --> 00:10:46,340
+للاستفيلو كوكاية كيف صار الـ differential؟ أضفنا
+
+153
+00:10:46,340 --> 00:10:52,510
+عليه Manitol ومع الـ Manitol pH indicator الآن لون
+
+154
+00:10:52,510 --> 00:10:56,850
+الوسط الأصلي بنات شوفوا اللون الزهري هذا هو ده
+
+155
+00:10:56,850 --> 00:11:01,810
+اللون الأصلي للوسط لما نمنا عليه البكتيريا مين
+
+156
+00:11:01,810 --> 00:11:04,910
+اللي هننمو؟ الـ Staphylococci اللي عندها مقدرة اللي
+
+157
+00:11:04,910 --> 00:11:10,790
+هتنمو عليها أعلى من 7.5% Sodium Chloride طيب الـ
+
+158
+00:11:10,790 --> 00:11:14,010
+Staphylococci في منها نوعين نوع بيعمل Manitol
+
+159
+00:11:14,010 --> 00:11:17,230
+Fermentation زي الـ Staphylococcus aureus شفتوا صورة
+
+160
+00:11:17,230 --> 00:11:19,730
+.. شفتوا التلاتة فتخلصوا اتحكيهم قبل شوية؟ منهم
+
+161
+00:11:19,730 --> 00:11:24,490
+Manitol Fermentation وباقي الستفيلو كوكاي are non
+
+162
+00:11:24,490 --> 00:11:30,110
+manitol fermenter إذا الـ manitol fermenter بكتيريا
+
+163
+00:11:30,110 --> 00:11:33,930
+نمت على هذا الوسط هتخفض الـ pH as a result of the
+
+164
+00:11:33,930 --> 00:11:38,010
+acid produced by manitol fermentation انخفاض الـ pH
+
+165
+00:11:38,010 --> 00:11:45,290
+هيحول لون الوسط من زهري إلى أصفر إذا بتلاحظوا في
+
+166
+00:11:45,290 --> 00:11:48,690
+المثلثات اللي موجودة قدامكم باللون الأصفر هذي دي
+
+167
+00:11:48,690 --> 00:11:52,450
+دلالة على إنّ البكتيريا .. على إنّ البكتيريا منطال
+
+168
+00:11:52,450 --> 00:11:55,350
+.. خلّيني أكون متواضعين دائمًا في التعرف على
+
+169
+00:11:55,350 --> 00:11:59,530
+البكتيريا نحاول نوصف وصفًا دقيقًا، هي منطال fermented
+
+170
+00:11:59,530 --> 00:12:03,490
+إذا كنا عاملينها كتاليز، بنقول عنها كتاليز بصدر،
+
+171
+00:12:03,490 --> 00:12:06,270
+إذا كنا عاملين جرام استنقلها، هتكون جرام
+
+172
+00:12:10,450 --> 00:12:14,070
+يبقى الاحتمال الأكبر هتكون Staphylococcus aureus
+
+173
+00:12:14,070 --> 00:12:17,530
+أعتقد إنّ هذه الصورة واضحة، لذا هذا وسط رائع جدًّا
+
+174
+00:12:17,530 --> 00:12:23,490
+لعزل البكتيريا من بيئات تحتوي على أنواع أخرى من
+
+175
+00:12:23,490 --> 00:12:27,970
+البكتيريا، بيمنع لي الـ others وبيعرفني لـ
+
+176
+00:12:27,970 --> 00:12:32,330
+Staphylococcus aureus، طب ناخد له تطبيق هناك بعض
+
+177
+00:12:32,330 --> 00:12:35,930
+الأغذية بنات، بعض الأغذية، من غير مسموح أن يكون
+
+178
+00:12:35,930 --> 00:12:40,770
+فيها Staphylococcus aureus إلا بعدد أقل من 100، من
+
+179
+00:12:40,770 --> 00:12:44,190
+100 لكل جرام من الغذاء، فكيف أعدّ Staphylococcus aureus؟ بعدها على وسط selective
+
+180
+00:12:44,190 --> 00:12:47,550
+and differential زي المنطول، salt agar، ليش؟ لأنّه
+
+181
+00:12:47,550 --> 00:12:52,790
+بيمنع الميكروبات الأخرى وبيمنع نموها وبيميزها بلون
+
+182
+00:12:52,790 --> 00:12:59,850
+الأصفر اللي هو نتيجة عن اللي مانت هو الـ
+
+183
+00:12:59,850 --> 00:13:01,830
+fermentation إذا من موضوعات كتيرة بهم سبق لنا في
+
+184
+00:13:01,830 --> 00:13:05,250
+النظري أو في العمل فحص الـ DNA's بندور للصورة
+
+185
+00:13:05,250 --> 00:13:11,010
+الأسفل هي الشائعة اللي هتستخدموها ركزوا معايا بس
+
+186
+00:13:11,010 --> 00:13:14,610
+شوية بالأول عشان نشرح الفكرة بتلعبوا الـ DNA أنتم
+
+187
+00:13:14,610 --> 00:13:20,850
+الـ DNA إيه؟ في بعض الأغذية بنسميها DNA-rich foods
+
+188
+00:13:20,850 --> 00:13:26,250
+من ضمنها البقوليات Serials الآن بيحطوا كمية من الـ
+
+189
+00:13:26,250 --> 00:13:34,150
+soy beans اللي هي أحد البقوليات هنا في الوسط يبقى
+
+190
+00:13:34,150 --> 00:13:39,430
+وسط مبني على soy beans مليان DNA مليان DNA الوسط
+
+191
+00:13:39,430 --> 00:13:44,290
+اللي لما كنتم تستخدموه في المعمل لو طلعتوا على الـ
+
+192
+00:13:44,290 --> 00:13:46,810
+Miller-Hinton أو على الـ nutrient agar اللي بتلاقوه
+
+193
+00:13:46,810 --> 00:13:49,290
+شفاف transparent لإيش؟ لأنّه مأخوذ من مواد ذائبة
+
+194
+00:13:49,290 --> 00:13:54,600
+والمواد الذائبة ما بتعملش turbidity ما بتعملش
+
+195
+00:13:54,600 --> 00:13:58,660
+turbidity ذائبة كلّيًا الآن لو أضفنا على الوسط
+
+196
+00:13:58,660 --> 00:14:03,160
+العالي الطبيعي حتى يبقى شفاف زرعنا عليه البكتيريا
+
+197
+00:14:03,160 --> 00:14:06,420
+اللي بنفحصها شكل نقطة مش ذرّاعة عادية نقطة عملنا
+
+198
+00:14:06,420 --> 00:14:11,600
+نقطة والمستعمرة بدأت تكبر وبعد 24 ساعة طلعنا على
+
+199
+00:14:11,600 --> 00:14:15,780
+الطبق زي ما هو ما قدرناش نعرف إنّ البكتيريا بتنتج DNA
+
+200
+00:14:15,780 --> 00:14:19,020
+أو ما تنتجش DNA كيف نعرف أضفنا point واحد أو واحد
+
+201
+00:14:19,020 --> 00:14:24,960
+مربع عفوًا HCl إيش بيعمل HCl الـ HCl بيعمل
+
+202
+00:14:24,960 --> 00:14:30,800
+denaturation للـ protein لما يعمل .. أفضل للـ DNA ..
+
+203
+00:14:30,800 --> 00:14:35,850
+لما يعمل denaturation للـ DNA بيعمل turbidity في
+
+204
+00:14:35,850 --> 00:14:40,090
+الوسط، لاحظوا الوسط هنا بطل clear، صح؟ وين فيه
+
+205
+00:14:40,090 --> 00:14:46,810
+clear؟ حوالي المستعمرة هذه وحوالي المستعمرة هذه،
+
+206
+00:14:46,810 --> 00:14:52,890
+لكن حوالي المستعمرة هذه فيه clear؟ طب اللي فيها
+
+207
+00:14:52,890 --> 00:14:56,350
+clear ليش فيها clear؟ لأنّه مش فيها DNA وأراها الـ
+
+208
+00:14:56,350 --> 00:15:00,560
+DNA تكسّرت بفعل الـ DNA's اللي قدرته البداية وصل
+
+209
+00:15:00,560 --> 00:15:05,020
+الصورة لأنّه بيقدر نميّز DNA's بُصلف عن DNA's
+
+210
+00:15:05,020 --> 00:15:09,760
+negative في وسط اسمه DNA's أجار باستخدام واحد
+
+211
+00:15:09,760 --> 00:15:14,580
+normal HCl متى بعد ما تخلص عملية الـ incubation
+
+212
+00:15:14,580 --> 00:15:19,000
+أنتم عملتوا الـ start شان دول لسه اللي لسه في
+
+213
+00:15:19,000 --> 00:15:21,780
+المعمل؟
+
+214
+00:15:21,780 --> 00:15:22,340
+ 
+215
+00:15:28,350 --> 00:15:31,850
+الـ amylase فكرته إنّه نزرع البكتيريا على وسط
+
+216
+00:15:31,850 --> 00:15:37,290
+فيه starch وبعد 24 ساعة بنعمل flooding للطبق بالـ
+
+217
+00:15:37,290 --> 00:15:41,830
+IOD's الـ IOD's مش بيعمل بيرضبط مع الـ starch بيكوّن
+
+218
+00:15:41,830 --> 00:15:45,390
+لون أزرق زي اللي زي المشهورة اللي أخذناها زمان
+
+219
+00:15:45,390 --> 00:15:49,310
+المنطقة اللي خالية من الـ starch بتحضر colorless
+
+220
+00:15:49,310 --> 00:15:54,800
+نفس الفكرة وأربع جنسها وبعدين نظفنا اللي هو one
+
+221
+00:15:54,800 --> 00:15:59,500
+normal HCL صار turbidity حوالي المستعمرة negative ما
+
+222
+00:15:59,500 --> 00:16:03,040
+صار turbidity حوالي المستعمرة الفحص positive الصور
+
+223
+00:16:03,040 --> 00:16:06,400
+اللي فوق تقنية بيستخدموا فيها indicator مختلف
+
+224
+00:16:06,400 --> 00:16:11,380
+فبالتالي الـ interpretation بتكون مختلفة كمان نقطة
+
+225
+00:16:11,380 --> 00:16:14,220
+مهمة دلوقت لـ Staphylococcus aureus أو لبعض أنواع من
+
+226
+00:16:14,220 --> 00:16:16,680
+البكتيريا اللي بتستخدم مركب
+
+227
+00:16:21,620 --> 00:16:25,720
+البوتاسيوم تليوريد مركب بيصير له reduction من بعض
+
+228
+00:16:25,720 --> 00:16:28,700
+البكتيريا reduction يعني utilization يعني استخدام
+
+229
+00:16:28,700 --> 00:16:33,060
+مش كلّ البكتيريا تقدر تعمل reduction few bacteria
+
+230
+00:16:33,060 --> 00:16:36,860
+can produce this substance أو use this substance
+
+231
+00:16:36,860 --> 00:16:41,180
+منهم من الـ Staphylococcus aureus لما بتعمله
+
+232
+00:16:41,180 --> 00:16:44,680
+reduction ويكون في الوسط هو ويكون colorless بيصير
+
+233
+00:16:44,680 --> 00:16:49,840
+لونه أسود فالمستعمرات بيصير لونها أسود فبالتالي
+
+234
+00:16:49,840 --> 00:16:53,230
+برضه بميز مين؟ الستافيلوكوكاس أو رياسة عن
+
+235
+00:16:53,230 --> 00:16:56,270
+الميكروبات الأخرى بلون المستعمرة اللي هو اللون
+
+236
+00:16:56,270 --> 00:17:00,450
+الأسود اللي أنا بوصلكم إياه ده رسالة إنّ في أوساط
+
+237
+00:17:00,450 --> 00:17:04,410
+selective غير الـ manitol salt agar وفكرة أخرى ده
+
+238
+00:17:04,410 --> 00:17:08,370
+اختلف عن الـ manitol salt agar لكن معتمد على مين؟
+
+239
+00:17:08,370 --> 00:17:11,570
+على الـ potassium telluride الـ manitol salt agar
+
+240
+00:17:11,570 --> 00:17:15,290
+بقى معتمد على مين؟ على الـ sodium chloride النسبة
+
+241
+00:17:15,290 --> 00:17:20,030
+العالية اللي هي 7.5% هذا فحص جديد مش هتاخدوه في
+
+242
+00:17:20,030 --> 00:17:24,550
+المعمل، فياريت تطلعوا عليه كويس دائمًا أبدأ بعد
+
+243
+00:17:24,550 --> 00:17:27,650
+نقطع شهود إحنا في الـ micro biology في معلومات
+
+244
+00:17:27,650 --> 00:17:32,470
+بتصير مسلّمات بنحاول إنّه نعطيكم بعض الـ exceptions
+
+245
+00:17:32,470 --> 00:17:35,810
+جرعات من الـ exceptions، كويس أحسن من إنّه نعطيكم
+
+246
+00:17:35,810 --> 00:17:38,750
+الـ exception في نفس اليوم لما كنا نقول
+
+247
+00:17:38,750 --> 00:17:43,210
+Staphylococcus و Streptococcus عنوانهم ground
+
+248
+00:17:43,210 --> 00:17:46,250
+positive coccyte إحنا كنا الصحيح ما نقولش كلّ الحقيقة
+
+249
+00:17:47,460 --> 00:17:52,580
+في كمان مجموعة .. في كمان مجموعة .. في كمان مجموعة
+
+250
+00:17:52,580 --> 00:17:57,340
+اسمها micrococcus micrococcus يبقى micrococcus,
+
+251
+00:17:57,520 --> 00:18:01,740
+staphylococcus و streptococcus ما المشترك بينهما؟
+
+252
+00:18:01,740 --> 00:18:06,100
+staphylococcus و الـ micrococcus الكتاليزي أنهم 
+
+253
+00:18:06,100 --> 00:18:09,580
+Gram positive cocci و الكتاليزي بوستيف كبنا ميز
+
+254
+00:18:09,580 --> 00:18:12,320
+بينهم هذا الفحص اللي قدامكم اسمه الـ lysostaphin
+
+255
+00:18:13,730 --> 00:18:17,230
+Lysostaphin واضح من اسمه إنه بيعمل lysis لمين؟
+
+256
+00:18:17,230 --> 00:18:22,510
+للاستاف، كل الاستاف بتدوب، كده إيش يعني بتدوب؟
+
+257
+00:18:22,510 --> 00:18:25,950
+يعني بتموت و بتتحلل، بتموت و بتتحلل، أصلا الـ
+
+258
+00:18:25,950 --> 00:18:29,150
+Penicillin عارفين كيف بيقتل البكتيريا اللي بيقتل
+
+259
+00:18:29,150 --> 00:18:32,990
+الاستافيلو كوكس اوريوس برضه بتتحلل، أصلا اكتشافه
+
+260
+00:18:32,990 --> 00:18:37,470
+البنسلين من قبل Alexander Fleming كان بطريقة هذه
+
+261
+00:18:37,470 --> 00:18:40,430
+فطر نمى جنب الـ ... الـ ... الـ Staphylococcus aureus
+
+262
+00:18:40,430 --> 00:18:45,150
+دول المستعمرات اللي أصلا كانت موجودة فالبكتيريا
+
+263
+00:18:45,150 --> 00:18:48,950
+ممكن إذا الـ cell wall تبعها ات... اتحلل و أنتم
+
+264
+00:18:48,950 --> 00:18:51,550
+عارفين البنسلين مش بتعمل إيه؟ على الـ cell wall
+
+265
+00:18:51,550 --> 00:18:54,930
+طب كيف بنعرف إن الـ ... الـ ... اتحلل ... البكتيريا
+
+266
+00:18:54,930 --> 00:18:57,830
+ولا اتحللت إيش؟ بنطلع على أنبوبة و هذا ... هذا يعني
+
+267
+00:18:57,830 --> 00:19:01,910
+نقطة كتير مهمة بنستخدمها احنا في الـ comparisons
+
+268
+00:19:01,910 --> 00:19:09,230
+بنجيب مثلًا ورقة مطبوعة تمام و بنحط أنبوبتين قدام
+
+269
+00:19:09,230 --> 00:19:14,730
+الورقة و بنقرأ الكلام المطبوع من خلال الأنابيب في
+
+270
+00:19:14,730 --> 00:19:17,470
+هذه الحالة ما بنقرأش احنا قاعدين هنا نقرأ وضوح
+
+271
+00:19:17,470 --> 00:21:350
+الخطوط فقط ما بنقرأش كلام نقرأ وضوح الخطوط مين
+
+272
+00:19:21,350 --> 00:19:26,830
+أوضح؟ واضح إن هنا الخط واضح جدًا بينما هنا يعني الـ
+
+273
+00:19:26,830 --> 00:19:29,370
+turbidity هنا أعلى من الـ turbidity هنا
+
+274
+00:19:45,260 --> 00:19:50,300
+مؤشر على أن البكتيريا اللي نميها هنا لم تتحلل ماذا
+
+275
+00:19:50,300 --> 00:19:53,280
+يعني ذلك؟ أنها resistant ماذا يعني ذلك؟
+
+276
+00:19:53,280 --> 00:20:00,680
+micrococcus فوق البكتيريا فقدت تمامًا من الأنبوبة
+
+277
+00:20:01,950 --> 00:20:06,190
+وبالتالي هي sensitive وبالتالي هي stiff وصلت الصورة
+
+278
+00:20:06,190 --> 00:20:10,490
+هذا فحص كتير مهم لأنه بيميز بين two organisms
+
+279
+00:20:10,490 --> 00:20:16,430
+بيشبهوا بعض بدرجة كبيرة أو درجات الشبه غير أنهم 
+
+280
+00:20:16,430 --> 00:20:19,590
+Gram positive cocci وكاتاليز بوستيف كمان على الطبق
+
+281
+00:20:19,590 --> 00:20:24,050
+they may look similar يعني الاستافيلو كوكس
+
+282
+00:20:24,050 --> 00:20:28,070
+والميكرو كوكس ممكن يكونوا أشكال مستعمرات قريبة من
+
+283
+00:20:28,070 --> 00:20:33,810
+بعضهم البعض فواجب علينا أن نحن نميز بينهم لـ...
+
+284
+00:20:33,810 --> 00:20:40,290
+الـ ... الـ ... الـ ... الـ rapid tests في المعمل
+
+285
+00:20:40,290 --> 00:20:44,330
+هتشتغلوا اندول بأنبوبة و Methyl Red في أنبوبة و
+
+286
+00:20:44,330 --> 00:20:46,810
+Voges Proskauer في أنبوبة و Citrate في أنبوبة و
+
+287
+00:20:46,810 --> 00:20:50,450
+Motility في أنبوبة يعني لو بدي أعمل عشرين فحص أنا
+
+288
+00:20:50,450 --> 00:20:53,690
+بدي عشرين أنبوبة بدي راك هالجدي و أنا بيب تقريبًا
+
+289
+00:20:53,690 --> 00:20:58,970
+عشرة صندوق طولها أو أكثر يعني عشان أعمل مجموعة ...
+
+290
+00:20:58,970 --> 00:21:02,530
+م... م... فحصات لمجموعة بكتيريا بعب الـ incubator
+
+291
+00:21:05,330 --> 00:21:09,890
+فالشركات لجأت إلى ما يسمى بالـ miniaturization يعني
+
+292
+00:21:09,890 --> 00:21:15,630
+لكبير نخليه لصغير زي ما بنعمل مجسمات لعمارة
+
+293
+00:21:15,630 --> 00:21:18,850
+الجدران احنا عملنا مجسمات لفحوصات صغيرة بس مجسمات
+
+294
+00:21:18,850 --> 00:21:24,270
+حقيقية و فعالة شغالة تمام شايفين الشريط هذا اللي
+
+295
+00:21:24,270 --> 00:21:28,810
+قدامنا اسمه الـ API وانسوا الباقي API Analytical
+
+296
+00:21:28,810 --> 00:21:32,930
+Profile Index من شركة فرنسية مشهورة اسمها Biomerieux
+
+297
+00:21:34,600 --> 00:21:40,360
+entered مجموعة من الـ strips هذه لمجموعات مختلفة من
+
+298
+00:21:40,360 --> 00:21:45,440
+البكتيريا يعني مثلًا عندنا API-20E للانتروبكترياسي
+
+299
+00:21:45,440 --> 00:21:50,040
+والنانثارمينتر عندنا API-staph للستافيلو كوكس عندنا
+
+300
+00:21:50,040 --> 00:21:54,340
+API-strip للستريبتو كوكس عندنا API-anaerobes
+
+301
+00:21:54,340 --> 00:21:58,260
+للبكتيريا اللي هوائية عندنا API-Neisseria تبقى فينا
+
+302
+00:21:58,260 --> 00:22:03,270
+أنواع مختلفة من إيه؟ من الـ API الـ API هو مسمى للـ
+
+303
+00:22:03,270 --> 00:22:07,890
+system نفسه لكن الـ system موزع على أنواع مختلفة من
+
+304
+00:22:07,890 --> 00:22:11,810
+اللي هو البكتيريا طيب يالا بدنا نحفظ الـ positive و
+
+305
+00:22:11,810 --> 00:22:17,990
+الـ negative تبعات هذول بتقدروا تحفظوهم؟ تقدروا؟
+
+306
+00:22:17,990 --> 00:22:23,170
+طيب لما أجيب لكم كمان 200 ... 200 ... 200 بكتيريا بـ
+
+307
+00:22:23,170 --> 00:22:28,650
+20 فحص هتكون العملية يعني معقدة فأنا مضطر أن ألجأ
+
+308
+00:22:28,650 --> 00:22:30,650
+إلى الـ internet قرر أن يكون هناك internet
+
+309
+00:22:34,650 --> 00:22:41,530
+في القاعة في برنامج اسمه ID backed سجله اسمه ID
+
+310
+00:22:41,530 --> 00:22:42,090
+backed
+
+311
+00:22:48,000 --> 00:22:53,960
+الفايل اللي مش موجود أنا هنزلكم نسخة على الفيسبوك
+
+312
+00:22:53,960 --> 00:22:57,520
+لكن عشان تقدر تنزله على ويندوز حديث هذا المصمم كان
+
+313
+00:22:57,520 --> 00:23:01,600
+لويندوز XP لازم تتطلعوا على اليوتيوب هذا عشان
+
+314
+00:23:01,600 --> 00:23:05,700
+تقدروا تعرفوا كيف بنعمل عملية التوافق للبرنامج وكيف
+
+315
+00:23:05,700 --> 00:23:07,140
+بنشغله هذا أنا قعدت
+
+316
+00:23:18,290 --> 00:23:23,850
+هو المفروض إنه بينزل بكل سهولة من الانترنت بعد
+
+317
+00:23:23,850 --> 00:23:24,330
+الشكل
+
+318
+00:23:32,000 --> 00:23:35,380
+عادة من كلكم قبولها بتنزيل الملفات وتشغيلها أو
+
+319
+00:23:35,380 --> 00:23:38,580
+تعمل set up يعني next next ما فيش أي مشكلة في
+
+320
+00:23:38,580 --> 00:23:41,680
+الموضوع بس تعرفون بتخزنوا الملف تبعكم وبعدين
+
+321
+00:23:41,680 --> 00:23:44,720
+تعملوا set up المشكلة في set up في برامج من النوع
+
+322
+00:23:44,720 --> 00:23:50,840
+هذا الدعم الفني توقف عند الـ XP والـ التالي بتدعمش
+
+323
+00:23:50,840 --> 00:23:55,440
+برامج اللي هم الـ ... الـ ... الـ Vista والـ Seven والـ
+
+324
+00:23:55,440 --> 00:23:58,520
+Eight في الـ Windows عشان تقدر تشتغل عليها لازم
+
+325
+00:23:58,520 --> 00:24:00,660
+نعمل حركة الحين بنشوفها كمان شوية
+
+326
+00:24:04,090 --> 00:24:08,310
+هنا بس عاملين بنختار الـ location لتنزيل الملف ملف
+
+327
+00:24:08,310 --> 00:24:24,990
+صغير جدًا لا يستغل ثواني في التحميل نأخذه
+
+328
+00:24:24,990 --> 00:24:28,870
+نحطه على الـ D هاي البرنامج
+
+329
+00:24:39,130 --> 00:24:47,550
+استكشاف مشاكل التوافق وإصلاحها أو خصائص شفت
+
+330
+00:24:47,550 --> 00:24:55,370
+التوافق؟ نختار تشغيل في وضع التوافق على ويندوز
+
+331
+00:24:55,370 --> 00:24:59,710
+جديد مثل ويندوز XP
+
+332
+00:24:59,710 --> 00:25:05,570
+بي تو إذا ما عملش الحركة دي مش هيشتغل البرنامج مش
+
+333
+00:25:05,570 --> 00:25:12,350
+هيشتغل البرنامج الآن خلاص هو جاهز للتشغيل بنشغله
+
+334
+00:25:12,350 --> 00:25:18,850
+كمسؤول كـ administrator يعني
+
+335
+00:25:18,850 --> 00:25:24,970
+هاي
+
+336
+00:25:24,970 --> 00:25:28,690
+set up زي ما حكيت لكم next next ما فيش حاجة special
+
+337
+00:25:28,690 --> 00:25:34,390
+في الموضوع وخلال ثانية واحدة تقريبًا بيكتمل لغتات
+
+338
+00:25:34,390 --> 00:25:43,070
+تبعته هاي
+
+339
+00:25:43,070 --> 00:25:50,910
+والآن صار في قائمة البرنامج طبعًا
+
+340
+00:25:50,910 --> 00:25:54,890
+البرنامج فيه إمكانيتين فيه إمكانية استخدام التعرف
+
+341
+00:25:54,890 --> 00:25:56,190
+قائمة التعرف
+
+342
+00:26:00,040 --> 00:26:04,480
+وفي هو أصلاً databases قواعد بيانات مجهزة ومجهزة
+
+343
+00:26:04,480 --> 00:26:09,100
+زي الـ API 10 الـ API 10 إذا بنفتح بهذه الطريقة
+
+344
+00:26:09,100 --> 00:26:12,880
+بيعطينا boxes شايفين الصناديق هذه بنعبيها الـ
+
+345
+00:26:12,880 --> 00:26:14,980
+positive والـ negative تبعنا اللي احنا حصلنا عليه
+
+346
+00:26:14,980 --> 00:26:17,820
+في المعمل من الـ strip positive negative negative
+
+347
+00:26:17,820 --> 00:26:20,940
+positive حسب النتائج اللي طلعت معنا وبضغطة واحدة
+
+348
+00:26:20,940 --> 00:26:25,020
+enter بيعطينا اسم الـ microbe ويبقينا جداش نسبة
+
+349
+00:26:25,020 --> 00:26:30,900
+التعرف بدقة على هذا الـ microbe مهم جدًا إنه نعرف
+
+350
+00:26:30,900 --> 00:26:35,560
+كيف نتعامل مع هذه البرامج لأنها جزء من المستقبل
+
+351
+00:26:35,560 --> 00:26:41,680
+تبعنا في الـ microbe لحظة الـ green واصل part
+
+352
+00:26:41,680 --> 00:26:44,760
+لتقريبًا 99% فبقى أنا أحكي الـ identification تبعت
+
+353
+00:26:44,760 --> 00:26:49,280
+هي قوية ومعرفة على أنها Vibrio cholera باقي أنا
+
+354
+00:26:49,280 --> 00:26:55,580
+أذكر كلمة واحدة هذه أجهزة برامج غبية سريعة احنا
+
+355
+00:26:55,580 --> 00:26:57,680
+اللي بنلقنها نعم احنا اللي بنعطيها المعلومات
+
+356
+00:26:57,680 --> 00:27:02,820
+وبترجع لنا بس بترجع لنا إياها بسرعة فإذا أنت شغلت غلط و
+
+357
+00:27:02,820 --> 00:27:05,860
+أعطيت أي حاجة أنا دخلت من عندي positive negative
+
+358
+00:27:05,860 --> 00:27:09,020
+positive و أعطاني إجابة عسب قواعد البيانات اللي
+
+359
+00:27:09,020 --> 00:27:12,760
+عنده فلازم نشتغل صح عشان لما أدخل صح يعطينا هو
+
+360
+00:27:12,760 --> 00:27:17,420
+برضه أيضًا تعريف صح هذا أحد البرامج اللي اسمه بنا
+
+361
+00:27:17,420 --> 00:27:23,300
+ID بقى سهل جدًا في التعامل معه ويمكن إضافة قواعد
+
+362
+00:27:23,300 --> 00:27:27,020
+بيانات من عندنا احنا نعمل قواعد بيانات خاصة فينا
+
+363
+00:27:27,020 --> 00:27:30,100
+وندخلها يعني لو أنا اتفهمت إن أنا تطورت system
+
+364
+00:27:30,100 --> 00:27:34,740
+جديد مش الـ API-20 عملت 10 فحص بدل 20 فحص سبعة
+
+365
+00:27:34,740 --> 00:27:38,520
+فحوصات بدل 20 فحص ممكن أدخلهم أنا أعمل قاعدة بيانات
+
+366
+00:27:38,520 --> 00:27:42,100
+وأدخلهم وسيلة استخدامها في التعرف على الـ micro
+
+367
+00:27:42,100 --> 00:27:48,420
+organism طيب نشوف اللي ... اللي قلنا عنه الـ PEP1
+
+368
+00:27:48,420 --> 00:27:54,580
+أنا
+
+369
+00:27:54,580 --> 00:27:58,640
+بحب دائمًا أضيف كلمة bacteria عشان ما يجيبوش هدا
+
+370
+00:27:58,640 --> 00:27:59,960
+التانية غير متوقعة
+
+371
+00:28:03,160 --> 00:28:06,480
+Prognostic هو اختصار الـ Prognostic identification
+
+372
+00:28:06,480 --> 00:28:07,960
+of bacteria for windows
+
+373
+00:28:16,120 --> 00:28:19,740
+من أجمل ... من أجمل برامج لل ... للـ researchers مش
+
+374
+00:28:19,740 --> 00:28:22,700
+الـ practical صحيح لل ... لل ... للـ عاملين لكن اللي
+
+375
+00:28:22,700 --> 00:28:25,880
+بيحب يتعرف على الميكروبات المجهولة من أي source من 
+
+376
+00:28:25,880 --> 00:28:28,920
+أي مصدر سواء بيئي أو طبي و كده بيستخدم هذا
+
+377
+00:28:28,920 --> 00:28:31,920
+البرامج أنا بأنصح في طلابي الماستر اللي بيستخدموا
+
+378
+00:28:31,920 --> 00:28:37,100
+بكتيريا غير .. أو مجهولة هم مش عارفينها يعني
+
+379
+00:28:37,100 --> 00:28:40,720
+بيدوروا على خاصية فيها مش على اسمها و بدهم لما
+
+380
+00:28:40,720 --> 00:28:45,740
+يلاقوا الخاصية يتعرفوا على الاسم بعد ذلك فممكن ناخد
+
+381
+00:28:46,380 --> 00:28:50,100
+نعمل download و ندور على المصفوفات اللي هي قواعد 
+
+382
+00:28:50,100 --> 00:28:53,660
+البيانات و عنده قائمة محترمة جدا من المصفوفات كلها
+
+383
+00:28:53,660 --> 00:28:58,420
+for free يعني برنامج for free بيساعدنا في التعرف
+
+384
+00:28:58,420 --> 00:29:02,170
+على الـ micro organism هنا اللي خدعناه أن نتكلم عن
+
+385
+00:29:02,170 --> 00:29:05,370
+البرامج اللي مستخدمة في التعرف على البكتيريا هو
+
+386
+00:29:05,370 --> 00:29:10,330
+وجود أنظمة زي الـ ABI صحيح سهلت علينا لكن في نفس
+
+387
+00:29:10,330 --> 00:29:14,690
+الوقت ذات اللي على الذاكرة البشرية وبتالي اضطررنا
+
+388
+00:29:14,690 --> 00:29:18,830
+نلجأ للبرامج المحوسبة اللي بتساعدنا بشكل كبير في
+
+389
+00:29:18,830 --> 00:29:20,770
+التعرف على هذه الميكروبات
+
+390
+00:29:25,460 --> 00:29:28,120
+احنا يعني طول الفترة السابقة أتكلم على
+
+391
+00:29:28,120 --> 00:29:31,340
+Staphylococcus aureus المكورة العنقودية الذهبية
+
+392
+00:29:31,340 --> 00:29:37,920
+إلها أخوات مهمين زي Staphylococcus epidermidis و
+
+393
+00:29:37,920 --> 00:29:44,420
+Staphylococcus saprophyticus لكن اللي بدأ أتكلم عنه
+
+394
+00:29:44,420 --> 00:29:50,340
+الآن أنه بكفي أن نخدم حق لـ Staphylococcus
+
+395
+00:29:50,340 --> 00:29:54,680
+epidermidis و Staphylococcus saprophyticus كنا نقول
+
+396
+00:29:54,680 --> 00:29:57,900
+إن الأورياس هي الباثوجين الوحيد في كل
+
+397
+00:29:57,900 --> 00:30:01,860
+Staphylococcus بعد فترة كنا لأ الأورياس الـ الـ
+
+398
+00:30:01,860 --> 00:30:05,260
+Epidermis بتسبب Endocarditis و بتسبب التهابات ممكن
+
+399
+00:30:05,260 --> 00:30:09,060
+في صمامات القلب السابروفايتكاس اللي جاية التهابات
+
+400
+00:30:09,060 --> 00:30:11,800
+منها سيستايتس اللي هي التهابات المثانة التهابات
+
+401
+00:30:11,800 --> 00:30:16,100
+المثانة طب الآن لو دورته على جوجل و كتبته
+
+402
+00:30:16,100 --> 00:30:19,200
+Coagulase Negative Staphylococcus و مين بينتمي على
+
+403
+00:30:19,200 --> 00:30:22,080
+Coagulase Negative Staphylococcus؟ التنتهي، مصبوف؟
+
+404
+00:30:23,270 --> 00:30:28,330
+هتلاقوا أنه وصلوا مراتب في بعض الأحيان أعلى من الـ
+
+405
+00:30:28,330 --> 00:30:31,370
+Staphylococcus aureus في نسبة إحداثهم للأمراض
+
+406
+00:30:31,370 --> 00:30:37,850
+المستشفية الـ Coagulase Negative Staphylococci نظرة
+
+407
+00:30:37,850 --> 00:30:40,350
+مختلفة عن السابق يعني الـ Coagulase Negative
+
+408
+00:30:40,350 --> 00:30:43,550
+Staphylococci أصبحت تنافس الـ Coagulase Positive
+
+409
+00:30:43,550 --> 00:30:46,910
+Staphylococci وأنا بقول لكم أكثر من دراسة منها في
+
+410
+00:30:46,910 --> 00:30:50,050
+غزة اللي جيناها بالفعل في بعض الأقسام في
+
+411
+00:30:50,050 --> 00:30:53,610
+المستشفيات الـ Coagulase Negative Staphylococci
+
+412
+00:30:53,610 --> 00:30:57,090
+كانت أعلى من اللي هو الـ Staphylococcus aureus فهي
+
+413
+00:30:57,090 --> 00:31:03,030
+في صعود والصعود تبعها ناجم عن أنه هي تمتلك مؤهلات
+
+414
+00:31:03,030 --> 00:31:07,470
+وزادت مؤهلاتها أكثر من أحصار الـ resistance للـ
+
+415
+00:31:07,470 --> 00:31:10,810
+antibiotic وبالتالي انتشارها في المستشفيات بيكون
+
+416
+00:31:10,810 --> 00:31:14,730
+واسع أنطق Major component اسمها Epidermis على
+
+417
+00:31:14,730 --> 00:31:17,450
+الجسم موجودة فهي major component of skin flora
+
+418
+00:31:17,450 --> 00:31:21,870
+opportunistic infection لكن الـ opportunistic كانت
+
+419
+00:31:21,870 --> 00:31:26,870
+بتزيد تذكروا دائما المستشفى بيه ألمين؟ للمرضى
+
+420
+00:31:26,870 --> 00:31:30,270
+للمرضى يعني لمين؟ ده اللي الـ immune system عندهم
+
+421
+00:31:30,270 --> 00:31:33,750
+immunocompromised وبالتالي الميكروبات الـ
+
+422
+00:31:33,750 --> 00:31:36,610
+opportunistic قادرة أن تعمل infection عند هذا
+
+423
+00:31:36,610 --> 00:31:39,770
+الناس الـ heart valves وقلنا فيها أن الـ
+
+424
+00:31:39,770 --> 00:31:42,310
+endocarditis يعني من الأمراض المشهورة لهذه
+
+425
+00:31:42,310 --> 00:31:45,790
+البكتيريا الـ identification ما بتعملش يملحظ على
+
+426
+00:31:45,790 --> 00:31:49,530
+blood agar does not ferment manitol non pigmented
+
+427
+00:31:49,530 --> 00:31:55,680
+و QMAS طبعا نجات لحظوا النقاط الماكتوبرت فيرماتيشي
+
+428
+00:31:55,680 --> 00:31:59,000
+نيجاتيف و Coagulase نيجاتيف في كمان نقطة تالتة
+
+429
+00:31:59,000 --> 00:32:03,920
+DNA's نيجاتيف DNA's نيجاتيف لأن ما تلاقي الـ
+
+430
+00:32:03,920 --> 00:32:09,640
+Staphylococcus aureus التلاتة بسيطة نيجي للميكروب
+
+431
+00:32:09,640 --> 00:32:13,040
+التاني اللي هو الـ Sabrophyticus و الـ Sabrophyticus
+
+432
+00:32:13,040 --> 00:32:17,400
+الصحيح يعني ارتباطه من لحد الآن في نوع واحد من
+
+433
+00:32:17,400 --> 00:32:20,160
+الأمراض اللي هو الـ urinary tract infection و
+
+434
+00:32:20,160 --> 00:32:25,700
+بالأخرى الـ cystitis اللي هي التهاب المثانة طبعا من
+
+435
+00:32:25,700 --> 00:32:30,680
+ميزاته أنه هو stuff كل صفات الـ stuff عنده ما عدا
+
+436
+00:32:30,680 --> 00:32:34,680
+الـ coagulase نيجاتيف طبعا و الـ manitol fermentation
+
+437
+00:32:34,680 --> 00:32:39,380
+نيجاتيف أيضا و الـ DNA نيجاتيف طب هذا بيصير هو و الـ
+
+438
+00:32:39,380 --> 00:32:42,460
+Staphylococcus epidermidis في نفس الخانة كيبنا
+
+439
+00:32:42,460 --> 00:32:47,600
+ملزمين عن بعض بيفحص اسمه Novobiocin الـ Novobiocin هو
+
+440
+00:32:47,600 --> 00:32:49,880
+يبقى عن الـ antibiotic يعني يبقى عن الـ antibiotic
+
+441
+00:32:49,880 --> 00:32:53,640
+بنستخدمه في الـ differentiation between saprophyticus
+
+442
+00:32:53,640 --> 00:32:59,820
+acid resistant و الـ epidermis بتكون sensitive طبعا
+
+443
+00:32:59,820 --> 00:33:03,870
+الصورة اللي قدامنا نعرض الصورة بتوضح أن القرص
+
+444
+00:33:03,870 --> 00:33:07,050
+الانتيباتيكي اللي هو نوغروفايوسين و حواليه zone of
+
+445
+00:33:07,050 --> 00:33:10,650
+inhibition منطقة خالية من النمو ده لأنه البكتيريا
+
+446
+00:33:10,650 --> 00:33:14,230
+اللي هنا sensitive بينما في الجزء الثاني من الطبق
+
+447
+00:33:14,230 --> 00:33:18,130
+مافيش zone of inhibition وبالتالي البكتيريا resist
+
+448
+00:33:18,130 --> 00:33:21,670
+طيب
+
+449
+00:33:21,670 --> 00:33:24,490
+نيجي لجزء الثاني من الموضوع اللي هو
+
+450
+00:33:28,700 --> 00:33:32,860
+بنطلقها على تنتين في أكثر من السلاسل يعني لو كانوا
+
+451
+00:33:32,860 --> 00:33:40,340
+تنتين برضه بنسميهم streptococci صور
+
+452
+00:33:40,340 --> 00:33:43,100
+توضحية هذا الصور اللي بيقولنا صورة توضيحية مش صورة
+
+453
+00:33:43,100 --> 00:33:46,280
+حقيقية هي عبارة عن رسم من تحت الـ microscope لكن
+
+454
+00:33:46,280 --> 00:33:51,980
+بتوضح لنا أن الـ chains طولها
+
+455
+00:33:51,980 --> 00:33:56,080
+مختلف قد تكون مكونة من تنتين بالفعل وهذا حسب الـ
+
+456
+00:33:56,080 --> 00:34:00,160
+handling الـ specimen يعني لو أنا أخذت أنبوبة و
+
+457
+00:34:00,160 --> 00:34:06,740
+زرعت فيها streptococcus by genes أنبوبة liquid و
+
+458
+00:34:06,740 --> 00:34:11,820
+ما عملتش أي disturbance ما حركتش خالص بعد 24 ساعة
+
+459
+00:34:11,820 --> 00:34:16,820
+لو يطلع عليها بيلاقيها عملة النمو تبعها يشبه ما
+
+460
+00:34:16,820 --> 00:34:19,680
+يسمى بالـ cotton ball cotton ball يعني زي كرات
+
+461
+00:34:19,680 --> 00:34:25,590
+بالقطن الكورة القطن من أيش كورة القطن؟ تخيلوا سلسلة
+
+462
+00:34:25,590 --> 00:34:30,810
+جاية تطول، إيه بتطول؟ في الآخر بتبقى لها مسكة حلوة
+
+463
+00:34:30,810 --> 00:34:36,830
+لك و لا تنحني؟ تنحني فبيصير تلف و تشبك بع بعضها و
+
+464
+00:34:36,830 --> 00:34:40,390
+بتعملي زي إيه؟ زي الكورة، لو أخدت قطرة بهدوء من
+
+465
+00:34:40,390 --> 00:34:44,990
+الأوحة تحت الميجروسكوب هتلاقي very long .. very long
+
+466
+00:34:44,990 --> 00:34:49,780
+chains يبقى طول السلسلة معتمدة على وين نمت
+
+467
+00:34:49,780 --> 00:34:52,600
+البكتيريا، إيش الظروف اللي اتعرضت لها، وعملتي الـ
+
+468
+00:34:52,600 --> 00:34:56,260
+handling، الـ handling اللي هي و أنا بضرع فيها، أو
+
+469
+00:34:56,260 --> 00:34:58,220
+عفوا مش بضرع فيها، بعمل general staining، مش بعمل
+
+470
+00:34:58,220 --> 00:35:01,720
+mixing أنا، الـ mixing هذا هو برضه بيكسر اللي هو الـ
+
+471
+00:35:01,720 --> 00:35:07,240
+.. الـ .. البكتيريا مذكركم بالـ beta alpha gamma
+
+472
+00:35:07,240 --> 00:35:10,620
+hemolysis احنا في استخدام الكوكاس أولى استخدامنا
+
+473
+00:35:10,620 --> 00:35:12,900
+صفة الـ beta hemolysis مرة واحدة إذا كنا beta
+
+474
+00:35:12,900 --> 00:35:16,480
+hemolytic أو non-beta hemolytic بس بدكم صحيح الـ
+
+475
+00:35:16,480 --> 00:35:21,740
+hemolysis أكثر استخدام له في الـ streptococci يعني
+
+476
+00:35:21,740 --> 00:35:27,080
+أقول لكم كمان تعبير أدق قد نصف البكتيريا في الـ
+
+477
+00:35:27,080 --> 00:35:29,700
+streptococcus بالـ hemolysis تبعها، عفاش ما بنقول
+
+478
+00:35:29,700 --> 00:35:33,040
+Alpha hemolytic streptococci Beta hemolytic
+
+479
+00:35:33,040 --> 00:35:38,160
+streptococci Gamma hemolytic streptococci يبقى
+
+480
+00:35:38,160 --> 00:35:41,480
+عملية تصنيف الـ streptococci دخلت فيها مين؟ الـ
+
+481
+00:35:41,480 --> 00:35:43,640
+hemolysis على الـ blood vessels، يعني ده فيها صفة
+
+482
+00:35:43,640 --> 00:35:48,380
+من الصفات المهمة جدا في الميكروبيولوجي دي أرفق و تدفأ
+
+483
+00:35:48,380 --> 00:35:51,440
+نقلب، و هذه الصفة شاركت مع مين؟ مع الـ
+
+484
+00:35:51,440 --> 00:35:54,540
+Staphylococcus Gram-positive chains or pairs
+
+485
+00:35:54,540 --> 00:35:59,840
+كتاليز نيجاتيف وبين بسين Staphylococci are كتاليز
+
+486
+00:35:59,840 --> 00:36:05,140
+بوستف مش معنى الـ Staphylococci وهي facultative عن
+
+487
+00:36:05,140 --> 00:36:10,600
+أيروب كتاليز بوستف ليش الـ Streptococci ما تكون
+
+488
+00:36:10,600 --> 00:36:12,960
+زيها كتاليز بوستف ما هي facultative عن أيروب و
+
+489
+00:36:12,960 --> 00:36:17,940
+بتتحمل وجود الـ oxygen بقول أن النظام الموجود فيها
+
+490
+00:36:17,940 --> 00:36:22,280
+بيختلف عن النظام الموجود في الـ Staphylococci في
+
+491
+00:36:22,280 --> 00:36:26,500
+الـ Staphylococci فيها SOD .. إيش الـ SOD؟ Super
+
+492
+00:36:26,500 --> 00:36:31,620
+Oxide Desmutase زائد كتاليز بينما في الـ
+
+493
+00:36:31,620 --> 00:36:36,740
+Streptococci فيها SOD زائد Peroxidase خدوا بالكم
+
+494
+00:36:37,110 --> 00:36:41,950
+الـ peroxidase والكتاليز يقوموا بنفس الوظيفة تحويل الـ
+
+495
+00:36:41,950 --> 00:36:48,530
+H2O2 إلى ماء زائد أكسجين بس في الكتاليز الأكسجين
+
+496
+00:36:48,530 --> 00:36:53,810
+بيطلع كموليكلر أكسجين في الـ peroxidase الأكسجين
+
+497
+00:36:53,810 --> 00:36:57,670
+بيستخدم مباشرة في الـ reduction لمركبات ثانية
+
+498
+00:36:57,670 --> 00:37:00,990
+فبيظهرش على أنه molecular أكسجين بجانب الفحص لو
+
+499
+00:37:00,990 --> 00:37:05,230
+حطينا بكتيريا عندها بيروكسيدايز على هيدروجين
+
+500
+00:37:05,230 --> 00:37:09,330
+بيروكسايد هل هيطلع bubbles؟ مش هيطلع bubbles لأن
+
+501
+00:37:09,330 --> 00:37:12,310
+مافيش فيها كاتاليز فيها بيروكسيدايز
+
+502
+00:37:15,510 --> 00:37:18,210
+اللوحة الصعبة اللي هنوجّه في عندها محاضرتنا في
+
+503
+00:37:18,210 --> 00:37:21,790
+الآخر لأنه مش هنقدر نخلصها في المحاضرة في الوقت 
+
+504
+00:37:21,790 --> 00:37:25,570
+المتبقي فاندها اسمها Streptomycin والـ
+
+505
+00:37:25,570 --> 00:37:30,030
+Streptomycin two types مكتوب عليهم واحد
+
+506
+00:37:30,030 --> 00:37:34,350
+Streptomycin S واحد اسمه Streptomycin O تعالوا
+
+507
+00:37:34,350 --> 00:37:38,070
+نطلع مع بعض على الصّفورة ونشوف إيش يعني
+
+508
+00:37:38,070 --> 00:37:38,990
+Streptomycin
+
+509
+00:37:45,050 --> 00:37:50,590
+O و Streptomycin S
+
+510
+00:37:50,590 --> 00:37:55,490
+من أين جاءت أحرف هذه الكلمات؟ من أين جاءت؟ ركزوا
+
+511
+00:37:55,490 --> 00:38:06,950
+معايا الاثنين لهم خاصية معينة تجاه الأكسجين هذا 
+
+512
+00:38:06,950 --> 00:38:10,890
+الأول Oxygen Labile
+
+513
+00:38:13,400 --> 00:38:18,340
+يعني بيتدمر، بيتأثر بالـ Oxygen بينما الثاني
+
+514
+00:38:18,340 --> 00:38:23,420
+Stable الأحرف
+
+515
+00:38:23,420 --> 00:38:30,140
+Oxygen Labile و هذه Oxygen Stable طبعًا التسمية
+
+516
+00:38:30,140 --> 00:38:35,780
+ممكن تكون مهمة، لكن برضه مش هي اللي المقصودة عنها
+
+517
+00:38:35,780 --> 00:38:41,860
+الـ Streptolysin لما
+
+518
+00:38:41,860 --> 00:38:45,230
+بدخل هذا الاسم الإنسان من ضمن البكتيريا اللي
+
+519
+00:38:45,230 --> 00:38:50,170
+بتنتجه، اسمنا بيكوّله Antibodies، إيش بنسميه 
+
+520
+00:38:50,170 --> 00:38:56,110
+Antibodies؟ Antistreptolysin، أو .. إذا
+
+521
+00:38:56,110 --> 00:39:00,030
+Streptolysin هو مين؟ هو الاندجين، وال Antibody
+
+522
+00:39:00,030 --> 00:39:04,310
+اسمه Antistreptolysin، مين اللي بنتجه أساسًا؟
+
+523
+00:39:09,130 --> 00:39:12,390
+The Streptococcus pyogenes number one cause of
+
+524
+00:39:12,390 --> 00:39:17,590
+bacterial tonsillitis فاكرين
+
+525
+00:39:17,590 --> 00:39:21,910
+التونسيلايتس؟ فيه هدافيكو يعني هرب منها في يوم من
+
+526
+00:39:21,910 --> 00:39:26,210
+الأيام خاصة في سن الطفولة معظمنا أصيب بها مرارا
+
+527
+00:39:26,210 --> 00:39:30,890
+وتكرارًا مرارًا وتكرارًا يبقى بتوقع أنه دخل عندنا ميم
+
+528
+00:39:30,890 --> 00:39:36,960
+ASO واتكون عندنا ASO يعني لو فحصناك والآن كلكوا
+
+529
+00:39:36,960 --> 00:39:40,500
+هتلاقي إنكوا ASO هذا لا يعني أنكوا الآن مصابين
+
+530
+00:39:40,500 --> 00:39:46,060
+بالاستربتوكوكس وبالتالي اضطرينا أن نلجأ إلى حيلة العد
+
+531
+00:39:46,060 --> 00:39:51,040
+أو قياس التركيز فَأضفنا حرف الـ T في الآخر صار اسم
+
+532
+00:39:51,040 --> 00:39:57,540
+الفحص ASOT والـ T معناها title والـ title معناها
+
+533
+00:39:57,540 --> 00:40:02,340
+concentration وبالتالي الفحص بطّل يقول positive
+
+534
+00:40:02,340 --> 00:40:07,080
+نيجاتيف صار يقيس يعني بيقيس كمية اللي هو الـ
+
+535
+00:40:07,080 --> 00:40:10,900
+antibodies للـ Streptolysin وبالتالي اسم الفحص الآن
+
+536
+00:40:10,900 --> 00:40:17,660
+اسمه Anti Streptolysin O طيب بيقيس تركيز هل هناك
+
+537
+00:40:17,660 --> 00:40:20,720
+علاقة بين التركيز وبين وجود إصابة حالية أو إصابة 
+
+538
+00:40:20,720 --> 00:40:25,620
+سابق نعم يوجد إذا كان إصابة سابقة غالبًا التركيز
+
+539
+00:40:25,620 --> 00:40:32,110
+بيكون أقل من 200 international units per ml إذا كان إصابة
+
+540
+00:40:32,110 --> 00:40:40,330
+حالية غالبًا يكون أكثر من 200 يبقى الآن صراحة نعرف
+
+541
+00:40:40,330 --> 00:40:44,130
+الفرق ما بين إصابة حالية وإصابة سابقة لأنه كان 
+
+542
+00:40:44,130 --> 00:40:49,530
+بالأول صعب صعب جدًا علينا نعرف لأن كل الناس أو معظم
+
+543
+00:40:49,530 --> 00:40:53,030
+الناس أصيبوا في يوم من الأيام بالستربتوكوكس
+
+544
+00:40:54,620 --> 00:40:57,560
+ووصف واضحة الصورة هذه أن الـ Antistreptolysin هو ..
+
+545
+00:40:57,560 --> 00:40:59,640
+واخدين بالكم من أحد البروتينات المشهورة جدًا في 
+
+546
+00:40:59,640 --> 00:41:03,740
+قطاع غزة للتعرف على أن مسبب اللوز هو
+
+547
+00:41:03,740 --> 00:41:08,740
+Streptococcus pyogenes كمان شغل مهم جدًا أنا قلت 
+
+548
+00:41:08,740 --> 00:41:13,880
+فيه تايتر، صح؟ التايتر لما بنجي نقيسه، احنا بنعطيه في 
+
+549
+00:41:13,880 --> 00:41:18,300
+التقرير للطبيب، بنعطي التقرير، بنقول أن التايتر
+
+550
+00:41:18,300 --> 00:41:23,280
+800 مثلًا، هو 200، 400، 800، 1600، اللي هو serial
+
+551
+00:41:23,280 --> 00:41:27,780
+dilution فكنا له 800 مثلًا الطبيب هنا لازم ياخد باله
+
+552
+00:41:27,780 --> 00:41:31,360
+من أنه الـ concentration عالية والأسبوع الماضي
+
+553
+00:41:31,360 --> 00:41:37,220
+تذكروا تحدثنا عن بعض الأمراض المرتبطة بالـ immunity
+
+554
+00:41:37,220 --> 00:41:43,500
+بمعنى آخر أن الميكروبات بتنتج انتيجينات شبيهة
+
+555
+00:41:43,500 --> 00:41:48,040
+بأنتيجيناتنا الـ antibodies اللي بتنتج مش بس بتهاجم
+
+556
+00:41:48,040 --> 00:41:52,440
+البكتيريا بتهاجم برضه ومن ضمن أسوأ الميكروبات
+
+557
+00:41:52,440 --> 00:41:55,820
+اللي هي الـ Streptococcus pyogenes تمام؟ وبالتالي
+
+558
+00:41:55,820 --> 00:41:59,000
+لما بيلاقي title عالي يبقى هذا المرض بيكون under
+
+559
+00:41:59,000 --> 00:42:02,880
+observation طيلة فترة العلاج حتى يمثل مع الـ
+
+560
+00:42:02,880 --> 00:42:07,560
+complications اللي احنا تحدثنا عنها فحص مهم فحص 
+
+561
+00:42:07,560 --> 00:42:11,060
+شائع عرفنا اليوم المصدر تبعه اللي هو الـ
+
+562
+00:42:11,060 --> 00:42:15,630
+Streptolysin واللي هو الخاص بمين؟ بالـ Streptococcus
+
+563
+00:42:15,630 --> 00:42:21,450
+pyogenes طيب في عندنا اللي هو الـ .. الـ .. الـ pyogenic
+
+564
+00:42:21,450 --> 00:42:24,170
+exotoxins وهو عبارة عن erythrogenic كلمة 
+
+565
+00:42:24,170 --> 00:42:26,450
+erythrogenic يعني واضح من أنه .. أنه بي .. بي ..
+
+566
+00:42:26,450 --> 00:42:30,210
+بيستهدف مين؟ اللي هو بيستهدف الـ .. الـ red blood
+
+567
+00:42:30,210 --> 00:42:34,190
+cell الـ hyaluronidase اللي قلنا بيفكفك الخلايا عن
+
+568
+00:42:34,190 --> 00:42:40,390
+بعضها البعض الـ Streptokinase ماروا معانا؟ kinases
+
+569
+00:42:40,390 --> 00:42:41,970
+عكس مين؟
+
+570
+00:42:44,690 --> 00:42:47,830
+عكس الكواغوليز، عكس الكواغوليز، قلنا الكواغوليز
+
+571
+00:42:47,830 --> 00:42:52,370
+ده ما بيحول الفيبرونيجن إلى فيبرين، هذا بيحول
+
+572
+00:42:52,370 --> 00:42:55,750
+الفيبرونيجن أو بيبام الـ lysis للفيبرونيجن، بيبام الـ
+
+573
+00:42:55,750 --> 00:42:59,590
+lysis لعفوا لما الفيبرين نقلت، وبالتالي بيعيق عملية
+
+574
+00:42:59,590 --> 00:43:02,650
+مين قلنا؟ عملية الـ healing للجروح، يعني الجروح ما
+
+575
+00:43:02,650 --> 00:43:07,620
+بتشفى، ما بتتكون جلطة الآن البكتيريا موجودة وبتنتج
+
+576
+00:43:07,620 --> 00:43:11,260
+Streptokinase وقلنا لكم أصبح Streptokinase هذا
+
+577
+00:43:11,260 --> 00:43:16,420
+بالضبط سلعة مهمة دوائية تستخدم في علاج الجذبات
+
+578
+00:43:16,420 --> 00:43:23,260
+للمرضى اللي بيصير عندهم اللي هو الجذبات في الـ
+
+579
+00:43:23,260 --> 00:43:27,020
+Streptodornase وهو عبارة عن deoxyribonuclease
+
+580
+00:43:27,020 --> 00:43:29,980
+يعني على غرار اللي هو الـ DNase تبع الـ
+
+581
+00:43:29,980 --> 00:43:35,060
+Staphylococcus Streptodornase اسمه وطبعًا هذا برضه 
+
+582
+00:43:35,060 --> 00:43:42,020
+له أهمية في عملية الحصول أو تكسير المادة 
+
+583
+00:43:42,020 --> 00:43:44,820
+النووية وبتاع الحصول عليها القواعد كغذاء
+
+584
+00:43:44,820 --> 00:43:52,420
+للـ بكتيريا فـ M-protein الـ M-protein المفروض
+
+585
+00:43:52,420 --> 00:43:55,700
+تكون فاكرينه؟ من ضمن من ضمن الـ virulence من
+
+586
+00:43:55,700 --> 00:44:00,820
+ضمن الـ virulence factors ويقولنا عنه antifagocytic
+
+587
+00:44:00,820 --> 00:44:06,740
+antifagocytic يعني بيمنع الفاجوسيتوز وبالتالي هو 
+
+588
+00:44:06,740 --> 00:44:11,760
+وسيلة جيدة من قبل البكتيريا لي حماية نفسها من اللي
+
+589
+00:44:11,760 --> 00:44:16,240
+حماية الفاجوسيتوز تذكروا بتكلم عن pyogenic
+
+590
+00:44:16,240 --> 00:44:20,900
+infection pyogenic infection يعني الـ pus إيش الـ
+
+591
+00:44:20,900 --> 00:44:21,040
+pus
+
+592
+00:44:26,430 --> 00:44:32,870
+النيوتروفيلز هي الفاجوسايتس فبالتالي بيعطي قدرة
+
+593
+00:44:32,870 --> 00:44:38,150
+للـ بكتيريا أنه يقتل الفاجوسايتس مش الفاجوسايتس هي
+
+594
+00:44:38,150 --> 00:44:42,990
+تقتل البكتيريا لأنه بيهرب من الفاجوسايتس طبعًا
+
+595
+00:44:42,990 --> 00:44:47,810
+غالبًا الـ immunity اللي بتنتج إنها بتكون نتيجة ضد
+
+596
+00:44:47,810 --> 00:44:51,970
+مين؟ ضد اللي هو الـ protein M يعني لو واحد جسمه كوّن
+
+597
+00:44:51,970 --> 00:44:54,790
+أجسام مضادة لـ protein M تمام هي اللي بتعطيه
+
+598
+00:44:54,790 --> 00:44:59,450
+immunity برضه هو مسؤول عن الـ strain variation يعني
+
+599
+00:44:59,450 --> 00:45:03,210
+البكتيريا ممكن تغير الـ protein M تبعها وبالتالي
+
+600
+00:45:03,210 --> 00:45:09,370
+تدخل في protein M1 وبعدين M2 M3 M4 وبالتالي 
+
+601
+00:45:09,370 --> 00:45:15,030
+بعطيها هي ما يسمى بـ strain variation تنوع السلالات
+
+602
+00:45:15,030 --> 00:45:21,810
+فكل مرة بيدخل الميكروب بـ strain جديد أنا عندي لـ M1
+
+603
+00:45:21,810 --> 00:45:27,850
+هو دخل لـ M2 من فـ M1 من فـ M1 صح؟ رقم تلاتة الـ
+
+604
+00:45:27,850 --> 00:45:31,410
+reinfection هو المسؤول عن الـ reinfection مدام هو
+
+605
+00:45:31,410 --> 00:45:34,070
+اللي بيعمل strain variation المفروض أن أنا أصبت
+
+606
+00:45:34,070 --> 00:45:38,070
+مرة واحدة ويتكوّن عندي مناعة تمام وانت هالموضوع
+
+607
+00:45:38,070 --> 00:45:42,630
+لكن أنا أصبت مرة تلو المرة بسبب ورود M protein
+
+608
+00:45:42,630 --> 00:45:46,310
+لأنه أخلف الـ strains وهو بتاعي سهل عاملت بيه الـ
+
+609
+00:45:46,310 --> 00:45:50,910
+reinfection كابسول برضه في بعض الـ .. الـ .. الـ
+
+610
+00:45:50,910 --> 00:45:53,950
+الستربتوكوكاية إلها كابسول وهي عبارة عن anti
+
+611
+00:45:53,950 --> 00:45:56,870
+-phagocytic غالبًا الـ strains اللي إلها كابسول
+
+612
+00:45:56,870 --> 00:46:01,670
+بتكون ميوكاين، من أشهر الستربتوكوكاية اللي إلها ..
+
+613
+00:46:01,670 --> 00:46:03,470
+من أشهر الستربتوكوكاية اللي إلها كابسول
+
+614
+00:46:10,920 --> 00:46:14,880
+الـ Streptococci مجموعة ضخمة، ضخمة ضخمة كبيرة في 
+
+615
+00:46:14,880 --> 00:46:18,860
+منها بناكلوها احنا مع اللبن، يعني بيستخدموها لـ ..
+
+616
+00:46:18,860 --> 00:46:23,500
+اسمها Streptococcus lactis Streptococcus lactis
+
+617
+00:46:23,500 --> 00:46:27,280
+إنها بتعمل الـ .. الـ .. الـ lactic acid من الحليب
+
+618
+00:46:27,280 --> 00:46:33,480
+ففي منها pathogenic وفي منها non pathogenic فتم 
+
+619
+00:46:33,480 --> 00:46:37,540
+تصنيفها بطريقتين، طريقة الـ hemolysis فاكرينها؟
+
+620
+00:46:37,540 --> 00:46:41,710
+Alpha, Beta, Gamma هو الطريقة الثانية هلا يلا
+
+621
+00:46:41,710 --> 00:46:45,090
+انبسطوا الآن العالمة اللي موجودة بكورة اسمها عالمة
+
+622
+00:46:45,090 --> 00:46:49,850
+اسمها Rebecca Lancefield Rebecca Lancefield أنضت
+
+623
+00:46:49,850 --> 00:46:53,930
+حياتها في تصنيف الستربتوكوكاي based on a C
+
+624
+00:46:53,930 --> 00:46:56,530
+-Polysaccharide موجود في الـ cell wall تبع الستربتو
+
+625
+00:46:56,530 --> 00:46:59,790
+كوكاي يعني صنفت البكتيريا based on antigenic
+
+626
+00:46:59,790 --> 00:47:02,990
+structure C-Polysaccharide موجود في جدار ال cell
+
+627
+00:47:02,990 --> 00:47:08,870
+wall وهو بقي عن surface antigen الآن
+
+628
+00:47:09,410 --> 00:47:12,530
+لما جينا نجمع كل ال microbiota اللى اسمها stirt
+
+629
+00:47:12,530 --> 00:47:17,150
+coccus اللى جينا أنه فيه بينطبق عليها ال system
+
+630
+00:47:17,150 --> 00:47:20,990
+تبع Rebecca Lancefield وفيه بينطبقش عليها فاش صموها
+
+631
+00:47:20,990 --> 00:47:25,990
+groupable و non groupable groupable يعني .. يعني
+
+632
+00:47:25,990 --> 00:47:30,430
+يمكن تصنيفها بتصنيف Rebecca Lancefield و الثانية
+
+633
+00:47:30,430 --> 00:47:34,550
+non groupable معناها تحكي أنه لا تمتلك الـ
+
+634
+00:47:34,550 --> 00:47:38,510
+Antigenات اللي بنى عليها Rebecca Lancefield اللي هو
+
+635
+00:47:38,510 --> 00:47:43,710
+تصنيفها حصل منها Non-Groupable من أهم المجموعات A,
+
+636
+00:47:43,870 --> 00:47:47,930
+B و D الـ C و الـ G و ال F أقل أهمية لكن اللي هم
+
+637
+00:47:47,930 --> 00:47:51,330
+الـ A و ال B و ال D هم اللي هندرسهم خلال المحاضرة
+
+638
+00:47:51,330 --> 00:47:54,210
+اللي جاية و هي بس بعد السؤال الأخرى في محاضرتنا
+
+639
+00:47:54,210 --> 00:47:57,450
+الـ Non-Groupable تشمل واحد من الميكروبات المهم جدا
+
+640
+00:47:57,450 --> 00:48:01,850
+اللي هي Streptococcus pneumoniae و إلها شبيه موجود في
+
+641
+00:48:01,850 --> 00:48:06,050
+الفيلم بشكل كبير اسمه Streptococcus Mutans و بتسبب
+
+642
+00:48:06,050 --> 00:48:10,710
+Dental Caries أو تسوس اللي هو الاسم باكن كل حاجة
+
+643
+00:48:10,710 --> 00:48:12,970
+انهينا محاضرتنا و إن شاء الله نكمل مرة القادمة
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/uz47-1oLtNU_postprocess.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/uz47-1oLtNU_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..23d22a0ee81a8b1932f43da14649ad6cb37df724
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/uz47-1oLtNU_postprocess.srt
@@ -0,0 +1,2572 @@
+1
+00:00:20,860 --> 00:00:25,120
+بسم الله الرحمن الرحيم نكمل مع بعض سويا الموضوع
+
+2
+00:00:25,120 --> 00:00:29,840
+بعنوان biogenic coccus وكلمة biogenic coccus
+
+3
+00:00:29,840 --> 00:00:33,480
+اتفقنا على انه بيربطها معنايا اساسيا ان البكتيريا
+
+4
+00:00:33,480 --> 00:00:37,780
+اللي بتتحدث عنها هي كوكساي كراويت الشكل ومن سماتها
+
+5
+00:00:37,780 --> 00:00:40,980
+انها بتعمل biogenic infection او ما يسمى بال pass
+
+6
+00:00:40,980 --> 00:00:46,830
+producing infectionاطلعنا على بعض الصور اللى بتوضح
+
+7
+00:00:46,830 --> 00:00:52,690
+الأشكال المختلفة اللى هندرسها تحت هذا الموضوع شفنا
+
+8
+00:00:52,690 --> 00:00:59,370
+شكلها رئيسيين اللى هم ال chains و ال clusters ال
+
+9
+00:00:59,370 --> 00:01:03,450
+chains و ال clusters في اللوحات اللى أمامكوا
+
+10
+00:01:03,450 --> 00:01:07,670
+معظمهم ل ال strip to koukai فقط الوحيدة اللى منهم
+
+11
+00:01:07,670 --> 00:01:12,830
+استفى ل koukai اللى على يمين الشاشة في الأعلى
+
+12
+00:01:18,420 --> 00:01:21,240
+وبدأنا بالحديثة عن الـ Staphylococci والـ
+
+13
+00:01:21,240 --> 00:01:24,940
+Staphylococci هي المجموع الأهم حقيقة من حيث عدد ال
+
+14
+00:01:24,940 --> 00:01:28,520
+infection دي اللي بتسببها كلمة عدد يعني بتتكلم
+
+15
+00:01:28,520 --> 00:01:32,180
+فيها frequency frequency يعني نسبة الإصابة كم مرة
+
+16
+00:01:32,180 --> 00:01:35,700
+بيوصب البشر بهذا ال micro لأنه هتكلم عن ال
+
+17
+00:01:35,700 --> 00:01:40,860
+Staphylococci برضه بأكتر لكن أكتر من ناحية الأنواع
+
+18
+00:01:40,860 --> 00:01:44,620
+وليس ال frequency أنواع ال infection ال
+
+19
+00:01:44,620 --> 00:01:49,680
+Staphylococciميزاتها كانت نجران بوستف فكنت تبقى
+
+20
+00:01:49,680 --> 00:01:53,340
+نقله ال arrangement بعتها in clusters واتفقنا أنه
+
+21
+00:01:53,340 --> 00:01:57,320
+لا نستطيع حكم على ال clusters إلا من خلال اللي هو
+
+22
+00:01:57,320 --> 00:02:00,720
+ال catalase test إذا كانت catalase بوستف هيستفيله
+
+23
+00:02:00,720 --> 00:02:04,560
+كوكايل it is catalase بوستف major component of the
+
+24
+00:02:04,560 --> 00:02:10,660
+nose and skin normal microbiota شوفنا أشكال إلها
+
+25
+00:02:10,660 --> 00:02:16,540
+من ال pure culture و أشكال من عينات صديقشفنا بحث
+
+26
+00:02:16,540 --> 00:02:19,320
+ال catalase اللى تحدثنا عنه المرة الماضية و قلنا
+
+27
+00:02:19,320 --> 00:02:21,480
+انه يبقى عن قطرة من hydrogen peroxide و ضعف إلى
+
+28
+00:02:21,480 --> 00:02:24,360
+البكتيريا إذا طلع عندنا ال bubbles فهي agostin إذا
+
+29
+00:02:24,360 --> 00:02:28,240
+ما طلع عندنا ال bubbles بتكون catalaseوبدأنا
+
+30
+00:02:28,240 --> 00:02:30,380
+بالحديث اللي على الوضع الأصعب اللي هو ال
+
+31
+00:02:30,380 --> 00:02:32,420
+pathogenesis و ال immunity للـ Staphylococcus و
+
+32
+00:02:32,420 --> 00:02:35,860
+اتكلمنا عن اللي هو ال capsule، اتكلمنا عن ال
+
+33
+00:02:35,860 --> 00:02:39,440
+protein A، اتكلمنا عن ال liquid glycan، اتكلمنا عن
+
+34
+00:02:39,440 --> 00:02:44,300
+ال clumping factor أو ال coagulase substance اللي
+
+35
+00:02:44,300 --> 00:02:48,920
+موجود في البكتيريا، وشوفنا صورة لل protein A كيف
+
+36
+00:02:48,920 --> 00:02:52,300
+بيجلب أو بيرتبط بال FC portion دبعه اللي هو ال
+
+37
+00:02:52,300 --> 00:02:56,430
+antibioticمجموعة من الـ enzymes والتوكسين برضه
+
+38
+00:02:56,430 --> 00:02:59,630
+لوحة كاملة وركزنا في آخر الرزقين اللي هم الـtoxic
+
+39
+00:02:59,630 --> 00:03:03,050
+shock syndrome toxin والـ enterotoxin واتفقنا على
+
+40
+00:03:03,050 --> 00:03:06,010
+أن الاتنين super antigens لكن واحد acted
+
+41
+00:03:06,010 --> 00:03:08,670
+systematically وواحد acted locally في ال
+
+42
+00:03:08,670 --> 00:03:13,830
+intestinal tract وهذا الشكل أو الصورة وضحت لنا ال
+
+43
+00:03:13,830 --> 00:03:16,830
+mechanism by which super antigen works by joining
+
+44
+00:03:16,830 --> 00:03:21,730
+two cells اللي هم ال T cell and اللي قلنا عنها ال
+
+45
+00:03:21,730 --> 00:03:27,710
+macrofageو برضه تعرجنا على بعض الأمراض اللي اللي
+
+46
+00:03:27,710 --> 00:03:30,070
+علاقة بال skin و ال systemic و قولنا إنه في ال
+
+47
+00:03:30,070 --> 00:03:33,190
+skin فيه pharyncles و فينا style اللي قولنا ..
+
+48
+00:03:33,190 --> 00:03:36,830
+اللي سميناها بالعربي شحات ال N-P-T-G و الحصف ال
+
+49
+00:03:36,830 --> 00:03:40,050
+Imidermal Necrolysis و ال exfoliative dermatitis و
+
+50
+00:03:40,050 --> 00:03:44,230
+تانية قولنا فيهم دتلة الخلايا تبعات الجلب و ال
+
+51
+00:03:44,230 --> 00:03:47,470
+mastitis اللي هي تهيأة التدري أو الضرر abscess
+
+52
+00:03:47,470 --> 00:03:50,970
+اللي قولنا عنها هي deep pharyncles تمام؟ بتكون
+
+53
+00:03:50,970 --> 00:03:57,440
+مولودة في .. إنه في ال ..الكبد او في الدماغ او في
+
+54
+00:03:57,440 --> 00:04:02,920
+اماكن لكن اتفقنا على ان كل ال infections هذه ليها
+
+55
+00:04:02,920 --> 00:04:03,140
+ال
+
+56
+00:04:06,090 --> 00:04:09,810
+ال deep tissues بتكون الإصابة جاية من wound
+
+57
+00:04:09,810 --> 00:04:12,790
+infection على سبيل المثال يعني مثلا إلتهاب جروح أو
+
+58
+00:04:12,790 --> 00:04:18,170
+كأن abscess خارجي أو فرنكلز ومن ثم تخرب البكتيريا
+
+59
+00:04:18,170 --> 00:04:22,230
+من مكانها الأصلي إلى الدم و بتعمل اللي هي سبتسيميا
+
+60
+00:04:22,230 --> 00:04:27,650
+ممكن تصل إلى مكان مختلف ومتنوع خفنا الصورة هذه
+
+61
+00:04:27,650 --> 00:04:31,110
+برضه و اتكلمنا عن ان هذه ال impetigo وقولنا ان هذا
+
+62
+00:04:31,110 --> 00:04:34,230
+very contagious ويمكن الأول مرة نستخدم مصطلح
+
+63
+00:04:34,230 --> 00:04:38,750
+contagiousInfectious معناها معضي بينتقل من إنسان
+
+64
+00:04:38,750 --> 00:04:42,510
+إلى إنسان أو من حيوان إلى إنسان Contagious هو معضي
+
+65
+00:04:42,510 --> 00:04:45,670
+لكن بدرجة أكتر من كلمة infectious بمعنى أخر أن
+
+66
+00:04:45,670 --> 00:04:51,230
+نسبة العدوى فيه بتكون سريعة وعالية وبرضه كمان
+
+67
+00:04:51,230 --> 00:04:53,990
+اتطرقنا لموضوع مهم جدا لما اتكلمنا عن ال food
+
+68
+00:04:53,990 --> 00:04:57,370
+poisoning وميزنا بين ال food poisoning و ال food
+
+69
+00:04:57,370 --> 00:05:02,290
+infectionوأكد على أن ال food poisoning ارتبط
+
+70
+00:05:02,290 --> 00:05:09,110
+تسميته بتناول الإنسان بتناوله طعام يحتوي على تكسين
+
+71
+00:05:09,110 --> 00:05:12,370
+مش بالضرورة على الميكروب يمكن الميكروب يكون قام
+
+72
+00:05:12,370 --> 00:05:16,350
+بالتكسين ومات لكن أنا تناولت فقط أو ال symptoms
+
+73
+00:05:16,350 --> 00:05:19,610
+اللي صارت عندنا من وين؟ من التكسين بينما ال food
+
+74
+00:05:19,610 --> 00:05:23,250
+infection تناولنا الغذاء فيه micro organism قد
+
+75
+00:05:23,250 --> 00:05:26,230
+يكون العدد قليل لكن كافي أن يحدث عندي أنا
+
+76
+00:05:26,230 --> 00:05:30,230
+infectionو growth و بعدين الميكروب هو ينتج ال
+
+77
+00:05:30,230 --> 00:05:32,970
+toxin ويعمل disease في الداخل هذا سمينه food
+
+78
+00:05:32,970 --> 00:05:36,750
+infection فبنميز بين food poisoning و food
+
+79
+00:05:36,750 --> 00:05:40,910
+infection غالبا ال poisoning أعراضه بتبدأ سريعا
+
+80
+00:05:40,910 --> 00:05:44,170
+بينما ال infection قد تستمر يومين تلاتة أو تبدأ
+
+81
+00:05:44,170 --> 00:05:46,490
+بعد يومين أو تلاتة حسب نوع الميكروب اللي بيتكلم
+
+82
+00:05:46,490 --> 00:05:50,820
+عنههذه طبعا الصورة كانت لل toxic shock syndrome
+
+83
+00:05:50,820 --> 00:05:54,080
+لما اتكلمنا عن ال tampons وقلنا انه فيه مجموعة من
+
+84
+00:05:54,080 --> 00:05:56,940
+ال symptoms زي ال fever ال rashes و ال
+
+85
+00:05:56,940 --> 00:06:01,060
+disquamation و ال vomiting و ال diarrhea و ال
+
+86
+00:06:01,060 --> 00:06:04,120
+myelias و ان ال myelias اللي هي ألم العضلات
+
+87
+00:06:06,740 --> 00:06:09,560
+طبعا هذا الصورة ايضا اعتقد انها انهينا فيها
+
+88
+00:06:09,560 --> 00:06:12,260
+المحاضرة اللي اتكلمنا عنه اللي هو scalded skin
+
+89
+00:06:12,260 --> 00:06:16,600
+syndrome اللي جونا فيه توكسين اسمه X-folatein وهذا
+
+90
+00:06:16,600 --> 00:06:19,740
+ال X-folatein هو المسئول عن ال peeling off لل skin
+
+91
+00:06:19,740 --> 00:06:25,200
+تبع ال babies المصابينلا اعتقد ان احنا وصلنا ل ..
+
+92
+00:06:25,200 --> 00:06:29,060
+وصلنا لمرحلة و الصورة اللي قولنا عنها دي الصورة
+
+93
+00:06:29,060 --> 00:06:33,160
+اللي موجودة هي تعبيرة عن حالة واحدة اسمها Faj
+
+94
+00:06:33,160 --> 00:06:36,700
+typing يعني مش خاصة بالصفر ال Kukas ولكن معبرة عن
+
+95
+00:06:36,700 --> 00:06:40,120
+اي حالة اسمها Faj typing و الفراغات اللي احنا
+
+96
+00:06:40,120 --> 00:06:45,420
+شايفينها الصغيرة ها دي هي عبارة عن مناطق الفاج عمل
+
+97
+00:06:45,420 --> 00:06:48,660
+فيها infection و بالتالي صار فيها lysis سمناها
+
+98
+00:06:48,660 --> 00:06:55,310
+blacksأي Lysis من قبل الفاج يعني أن الفاج specific
+
+99
+00:06:55,310 --> 00:06:59,910
+للبكتيريا، إذا كان الفاج معروف والبكتيريا مجهولة
+
+100
+00:07:01,030 --> 00:07:05,310
+واتفقنا على انه يستخدم فيه عميق التعامل ثلاث صفات
+
+101
+00:07:05,310 --> 00:07:09,110
+للستفيلو كوكاس اوريس ذكرناهم المحاضرة السابقة بدنا
+
+102
+00:07:09,110 --> 00:07:13,150
+نأكد عليهم مرة أخرى manitol fermentation coagulase
+
+103
+00:07:13,150 --> 00:07:17,490
+test والديانيز ثلاث صفات positive تلاتة positive
+
+104
+00:07:17,490 --> 00:07:21,330
+بيميزوا الستفيلو كوكاس اوريس عن باقي أنواع
+
+105
+00:07:21,330 --> 00:07:25,750
+الستفيلو كوكا أحدهم بيسأل بيقول انه طيب بكفي ال
+
+106
+00:07:25,750 --> 00:07:29,290
+coagulase اللي خدته في المعمرهو الكوكاليش مميز من
+
+107
+00:07:29,290 --> 00:07:33,350
+الاستفيل الكوكا أول سباق الاستفيل الكوكا القاعش
+
+108
+00:07:33,350 --> 00:07:33,690
+بتقول
+
+109
+00:07:38,460 --> 00:07:41,380
+the more test you use the more accurate is your
+
+110
+00:07:41,380 --> 00:07:44,780
+diagnosis كل ما زاد عدد الفحوصات كل ما زاد دقة
+
+111
+00:07:44,780 --> 00:07:49,280
+التعرف لعل لو عشان نكون في microbe احنا مابنعرفه
+
+112
+00:07:49,280 --> 00:07:52,360
+أساسا يعني environmental microbe بيسمي ال microbe
+
+113
+00:07:52,360 --> 00:07:57,980
+بنعرفه وبالتالي لسنا بيصاد هنا انه نعمل تعريف غير
+
+114
+00:07:57,980 --> 00:08:02,980
+دقيق بدنا تعريف دقيق E ركزنا عليها ولا ماركزنا
+
+115
+00:08:02,980 --> 00:08:07,430
+عليها ال antibiotic sensitivityالـ Staphylococcus
+
+116
+00:08:07,430 --> 00:08:11,650
+aureus من الميكروبات المشهورة مشهورة المعنى آخر
+
+117
+00:08:11,650 --> 00:08:16,310
+يعني موجودة في مصاف النجوم في عالم الـ
+
+118
+00:08:16,310 --> 00:08:19,150
+Antimicrobial resistance من أوائل الميكروبات
+
+119
+00:08:19,150 --> 00:08:23,390
+أربعينية القرن الماضي البنسالين تم اكتشافه كان هو
+
+120
+00:08:23,390 --> 00:08:27,430
+ال magic لكل magic drug لكل Staphylococcal
+
+121
+00:08:27,430 --> 00:08:31,310
+infection بعد خمساشر سنة بدأنا نلاحظ resistance
+
+122
+00:08:31,310 --> 00:08:38,380
+طورنا ال penicillin وفي الآخرحصلنا على وضع مذير
+
+123
+00:08:38,380 --> 00:08:41,060
+للغاية شايفين الأسماء الأخيرة اللي موجودة على
+
+124
+00:08:41,060 --> 00:08:45,060
+اللوحة اللي هي penicillin, meticillin و vancobicin
+
+125
+00:08:45,060 --> 00:08:49,120
+اللي هون هي penicillin resistant بنسبة 95% ل 99%
+
+126
+00:08:49,120 --> 00:08:55,180
+في غزة عندنا هنا بنسبة من 95% ل 99% ال meticillin
+
+127
+00:08:55,180 --> 00:08:57,680
+وصلت ال resistance rate بعتها لأكتر من 40%
+
+128
+00:09:02,680 --> 00:09:06,680
+بواد الظهور ل Vancomycin-resistant Staphylococcus
+
+129
+00:09:06,680 --> 00:09:12,660
+aureus دراسة قبل أقل من شهر طلعت تلاتة و نص فالمية
+
+130
+00:09:12,660 --> 00:09:16,080
+يعني معناه الحاجة إن أنا فيها عندي مشكلة حقيقية
+
+131
+00:09:16,080 --> 00:09:18,880
+يعني هايجي يوم من الأيام لأنا استطيع أن أعالج
+
+132
+00:09:18,880 --> 00:09:21,040
+Staphylococcus aureus بال available drugs اللي
+
+133
+00:09:21,040 --> 00:09:24,380
+موجودة في الأسواق طبعا ال available drugs اللي هي
+
+134
+00:09:24,380 --> 00:09:30,560
+يمكن استخدامها استخدام للأدمالصورة اللي قدامنا هي
+
+135
+00:09:30,560 --> 00:09:33,200
+دي بتوضح ال hemolysis اللي حكينا عنه في المحاضرة
+
+136
+00:09:33,200 --> 00:09:36,920
+السابقة اللي هو beta .. beta hemolysis complete
+
+137
+00:09:36,920 --> 00:09:40,200
+hemolysis .. complete hemolysis الصورة هي بتوضح ان
+
+138
+00:09:40,200 --> 00:09:42,280
+ال .. ال .. مش ال hemolysis تعفو عنه ال manitol
+
+139
+00:09:42,280 --> 00:09:46,400
+salt agar هذا بده وقفة ويردت نتركز عنه شوية ال
+
+140
+00:09:46,400 --> 00:09:50,700
+manitol salt agar هو عبارة عن selective and
+
+141
+00:09:50,700 --> 00:09:54,120
+differential كلكوا بتعرف إيش أنا selective و إيش
+
+142
+00:09:54,120 --> 00:09:57,360
+أنا differentialالان كيف صار selective في هذا
+
+143
+00:09:57,360 --> 00:10:03,880
+الوساط؟ اضيف له 7.5% Sodium Chloride 7.5% Sodium
+
+144
+00:10:03,880 --> 00:10:09,520
+Chloride عارفين ايش يعني 7.5؟ يعني لما نحضر لتر
+
+145
+00:10:09,520 --> 00:10:14,980
+يعني 75 gram ده لتر يعني الملوحة تبعتها اعلى من
+
+146
+00:10:14,980 --> 00:10:21,320
+ملوحة البحر بضائفين تقريبا اعلى بقفل ضعف ملوحة
+
+147
+00:10:21,320 --> 00:10:25,860
+البحرمين ممكن ينمو في هذه ال concentration من ال
+
+148
+00:10:25,860 --> 00:10:29,260
+Sonium chloride لستفيلو الكوكاية؟ يبجأ المناعة
+
+149
+00:10:29,260 --> 00:10:34,280
+تنمو الميكروبات، الأخرى، مش بس ال Stripto، حتى
+
+150
+00:10:34,280 --> 00:10:37,280
+اللي امتلو بكترياسي المناعة تنموها لأنك لن تستطيع
+
+151
+00:10:37,280 --> 00:10:42,620
+تنمو في 7.5 يبجأ الوضع تصبح selective لمين؟
+
+152
+00:10:42,620 --> 00:10:46,340
+للاستفيلو الكوكاية كيف صار ال differential؟ أضافنا
+
+153
+00:10:46,340 --> 00:10:52,510
+عليه Manetol ومع ال Manetol pH indicatorالان لون
+
+154
+00:10:52,510 --> 00:10:56,850
+الوسط الأصلي بنات شفين اللون الزهري هذا هو ده
+
+155
+00:10:56,850 --> 00:11:01,810
+اللون الأصلي للوسط لما نمنا عليه البكتيريا مين
+
+156
+00:11:01,810 --> 00:11:04,910
+اللي هنمو؟ ال Staphylococci اللي عندها مقدرة اللي
+
+157
+00:11:04,910 --> 00:11:10,790
+هتنمو عليها أعلى من 7.5% Sodium Chloride طيب ال
+
+158
+00:11:10,790 --> 00:11:14,010
+Staphylococci في منها نوعين نوع بيعمل Menitol
+
+159
+00:11:14,010 --> 00:11:17,230
+Fermentation زي ال Staphylococcus aureus شفت صورة
+
+160
+00:11:17,230 --> 00:11:19,730
+.. شفتوا التلاتة فتخلصوا اتحكيهم قبل شوية؟ منهم
+
+161
+00:11:19,730 --> 00:11:24,490
+Menitol Fermentationوباقي الستفيلو كوكاي are non
+
+162
+00:11:24,490 --> 00:11:30,110
+manitol fermenter إذا ال manitol fermenter بكتيريا
+
+163
+00:11:30,110 --> 00:11:33,930
+نمت على هذا الوسط هتخفض ال pH as a result of the
+
+164
+00:11:33,930 --> 00:11:38,010
+acid produced by manitol fermentation انخفاض ال pH
+
+165
+00:11:38,010 --> 00:11:45,290
+هيحول لون الوسط من زهري إلى أصفرإذا بتلاحظوا في
+
+166
+00:11:45,290 --> 00:11:48,690
+المثلثات اللي موجودة قدامكوا باللون الأصفر هذي دي
+
+167
+00:11:48,690 --> 00:11:52,450
+لا علاج على إن البكتيريا .. على إن البكتيريا منطال
+
+168
+00:11:52,450 --> 00:11:55,350
+.. خلّيني أكون متواضعين دايما في التعرف على
+
+169
+00:11:55,350 --> 00:11:59,530
+البكتيريا نحاول نوصف وصف دقيق، هي منطال fermented
+
+170
+00:11:59,530 --> 00:12:03,490
+إذا كنا عاملينها كتاليز، بنقول عنها كتاليز بصدر،
+
+171
+00:12:03,490 --> 00:12:06,270
+إذا كنا عاملين جرام استنقلها، هتكون جرام
+
+172
+00:12:10,450 --> 00:12:14,070
+يبقى الاحتمال الاكبر هتكون Staphylococcus aureus
+
+173
+00:12:14,070 --> 00:12:17,530
+اعتقد ان هذا الصورة واضحة، لذا هذا بسط رائع جدا
+
+174
+00:12:17,530 --> 00:12:23,490
+لعزل البكتيريا من بيئات تحتوي على أنواع اخرى من
+
+175
+00:12:23,490 --> 00:12:27,970
+البكتيريا، بيمنعلي ال others و بعرفني ل
+
+176
+00:12:27,970 --> 00:12:32,330
+Staphylococcus aureus، طب ناخدله تطبيقهناك بعض
+
+177
+00:12:32,330 --> 00:12:35,930
+الأغضية بنات، بعض الأغضية، من غير مسموح أن يكون
+
+178
+00:12:35,930 --> 00:12:40,770
+فيها Staphylococcus aureus إلا بعدد أقل من 100، من
+
+179
+00:12:40,770 --> 00:12:44,190
+100 لكل gram من الغذاء، فكيف أدي عادل
+
+180
+00:12:44,190 --> 00:12:47,550
+Staphylococcus aureus؟ بعدها على وساط selective
+
+181
+00:12:47,550 --> 00:12:52,790
+and differential زي المنطول، salt، عجب، ليش؟ لأنه
+
+182
+00:12:52,790 --> 00:12:59,850
+بيبنعل الميكروبات الأخرىو بيناميلها و بيميزها بلون
+
+183
+00:12:59,850 --> 00:13:01,830
+الأصفر اللي هو نتجة عنه اللي مانت هو ال
+
+184
+00:13:01,830 --> 00:13:05,250
+fermentation إذا من وضعات كتير بهم سبق لنا في
+
+185
+00:13:05,250 --> 00:13:11,010
+النظري أو في العمل فحص ال DNA's بندنظر للصورة
+
+186
+00:13:11,010 --> 00:13:14,610
+الأسفل هي الشائعة اللي هتستخدموها تركزوا معايا بس
+
+187
+00:13:14,610 --> 00:13:20,850
+شوية بالأول عشان نشرح الفكرة بتلعبوا ال DNA انتوا
+
+188
+00:13:20,850 --> 00:13:26,250
+ال DNA ايه؟في بعض الأغذية بنسميها DNA-rich foods
+
+189
+00:13:26,250 --> 00:13:34,150
+من ضمنها البقليات Serials الآن بيحطوا كمية من ال
+
+190
+00:13:34,150 --> 00:13:39,430
+soy beans اللي هي أحد البقليات هنا في الوسط يبقى
+
+191
+00:13:39,430 --> 00:13:44,290
+وسط مبني على soy beans مليان DNA مليان DNA الوسط
+
+192
+00:13:44,290 --> 00:13:46,810
+اللي لما كنتوا تستخدموه في المعمل لو طلعتوا على ال
+
+193
+00:13:46,810 --> 00:13:49,290
+Miller-Hinton أو على ال nutrient exam اللي بتلاقوه
+
+194
+00:13:49,290 --> 00:13:54,600
+شفافtransparent لإيش؟ لأنه مأخوذ من مواد ذائبة
+
+195
+00:13:54,600 --> 00:13:58,660
+والمواد الذائبة مابتعملاش terminity مابتعملاش
+
+196
+00:13:58,660 --> 00:14:03,160
+terminity ذائبة كلية الآن لو اضطررت على الوسط
+
+197
+00:14:03,160 --> 00:14:06,420
+العالي الطبيعي حتى يبقى شفاف زرعنا على البكتيريا
+
+198
+00:14:06,420 --> 00:14:11,600
+اللى بنافحصهاشكل نقطة مش ذراعية عادية نقطة اعملنا
+
+199
+00:14:11,600 --> 00:14:15,780
+نقطة و المستعمرة بدت تكبر و بعد 24 ساعة طلعنا على
+
+200
+00:14:15,780 --> 00:14:19,020
+الطبق زي ما هو ماقدرنا نعرف ان البكتيريا بتنتج DNA
+
+201
+00:14:19,020 --> 00:14:24,960
+او ماتنتجش DNA كيبنا نعرف ضفنا point واحد او واحد
+
+202
+00:14:24,960 --> 00:14:30,800
+مربع عفوا HCl ايش بيعمل HCl ال HCl بيعمل
+
+203
+00:14:30,800 --> 00:14:35,850
+denaturation لل proteinلما يعمل .. أفضل لل DNA ..
+
+204
+00:14:35,850 --> 00:14:40,090
+لما يعمل denaturation لل DNA بيعمل turbidity في
+
+205
+00:14:40,090 --> 00:14:46,810
+الوسط، لاحظوا الوسط هنا بطل clear، صح؟ وين فيه
+
+206
+00:14:46,810 --> 00:14:52,890
+clear؟ حوالين المستعمر هذه وحوالين المستعمر هذه،
+
+207
+00:14:52,890 --> 00:14:56,350
+لكن حوالين المستعمر هذه فيه clear؟ طب اللي فيها
+
+208
+00:14:56,350 --> 00:15:00,560
+clearليش فيها clear؟ لأنه مش فيها DNA و اراها ال
+
+209
+00:15:00,560 --> 00:15:05,020
+DNA تكسّر بفعل ال DNA's اللي قدرته البداية وصل
+
+210
+00:15:05,020 --> 00:15:09,760
+الصورة لأنه بيقدر نميز DNA's بُصلف عن DNA's
+
+211
+00:15:09,760 --> 00:15:14,580
+negative في وسط اسمه DNA's أجار باستخدام واحد
+
+212
+00:15:14,580 --> 00:15:19,000
+normal HCl متى بعد ما تخلص عملية ال incubation
+
+213
+00:15:19,000 --> 00:15:21,780
+انتوا عملتوا ال startشان دول لسه اللي لسه في
+
+214
+00:15:21,780 --> 00:15:22,340
+المعمل؟
+
+215
+00:15:28,350 --> 00:15:31,850
+الألف أمياليزي فكرته أنه نزرع البكتيريا على وطط
+
+216
+00:15:31,850 --> 00:15:37,290
+فيه starch و بعد 24 ساعة بنعمل flooding للطبق بال
+
+217
+00:15:37,290 --> 00:15:41,830
+IOD's ال IOD's مش بيعمل بيرضبط مع ال starch بيكوّل
+
+218
+00:15:41,830 --> 00:15:45,390
+لون أزرق زي اللي زي المشهورة اللي أخدناها زمان
+
+219
+00:15:45,390 --> 00:15:49,310
+المنطقة اللي خالية من ال starch حضرها colorless
+
+220
+00:15:49,310 --> 00:15:54,800
+نفس الفكرةو أربع جنسها و بعدين نظفنا اللى هو one
+
+221
+00:15:54,800 --> 00:15:59,500
+normal HCL صار turbidity حول المستعمرة negative ما
+
+222
+00:15:59,500 --> 00:16:03,040
+صار turbidity حول المستعمرة الفحص positive الصور
+
+223
+00:16:03,040 --> 00:16:06,400
+اللى فوق تقنية بيستخدموا فيها indicator مختلف
+
+224
+00:16:06,400 --> 00:16:11,380
+فبالتالي ال interpretation بتكون مختلفة كمان نقطة
+
+225
+00:16:11,380 --> 00:16:14,220
+مهمية دلوقت لجوكاس اوريس او لبعض انواع من
+
+226
+00:16:14,220 --> 00:16:16,680
+البكتيريا اللى بتستخدم مركب
+
+227
+00:16:21,620 --> 00:16:25,720
+البوتاسيوم تليوريد مُركّب بصير له reduction من بعض
+
+228
+00:16:25,720 --> 00:16:28,700
+البكتيريا reduction يعني utilization يعني استخدام
+
+229
+00:16:28,700 --> 00:16:33,060
+مش كل البكتيريا تقدر تعمله reduction few bacteria
+
+230
+00:16:33,060 --> 00:16:36,860
+can produce this substance أو use this substance
+
+231
+00:16:36,860 --> 00:16:41,180
+منهم من الـ Staphylococcus aureus لما بتعمله
+
+232
+00:16:41,180 --> 00:16:44,680
+reduction ويكون في الوسط هو ويكون colorless بصير
+
+233
+00:16:44,680 --> 00:16:49,840
+لونه أسود فالمستعمرات بصير لونها أسود فبالتالي
+
+234
+00:16:49,840 --> 00:16:53,230
+برضه بميز مين؟الستافيلوكوكاس أو رياسة عن
+
+235
+00:16:53,230 --> 00:16:56,270
+الميكروبات الأخرى بلون المستعمر اللي هو اللون
+
+236
+00:16:56,270 --> 00:17:00,450
+الأسود اللي أنا بوصلكوا إياه ده رسالة إنه في أوساط
+
+237
+00:17:00,450 --> 00:17:04,410
+selective غير ال manitol salt agar وفكرة أخرى ده
+
+238
+00:17:04,410 --> 00:17:08,370
+اختلف عن ال manitol salt agar لكن معتمد على مين؟
+
+239
+00:17:08,370 --> 00:17:11,570
+على ال potassium telluride ال manitol salt agar
+
+240
+00:17:11,570 --> 00:17:15,290
+بقى معتمد على مين؟ على ال sodium chloride المسبة
+
+241
+00:17:15,290 --> 00:17:20,030
+العالى اللي هي 7.5%هذا فحص جديد مش هتاخده في
+
+242
+00:17:20,030 --> 00:17:24,550
+المعمل، فياريت تطلعوا عليه كويس دايما أبدا بعد
+
+243
+00:17:24,550 --> 00:17:27,650
+نقطع شهود احنا في ال micro biology في معلومات
+
+244
+00:17:27,650 --> 00:17:32,470
+بتصير مسلنات بنحاول أنه نعطيكوا بعض ال exceptions
+
+245
+00:17:32,470 --> 00:17:35,810
+جرعات من ال exceptions، كويس أحسن من أنه نعطيكوا
+
+246
+00:17:35,810 --> 00:17:38,750
+ال exception في نفس اليوم لما كنا نقول
+
+247
+00:17:38,750 --> 00:17:43,210
+Staphylococcus و Stryptococcus عنوانهم ground
+
+248
+00:17:43,210 --> 00:17:46,250
+mustard coccyte احنا كنا الصحيح مانقولش كل الحقيقة
+
+249
+00:17:47,460 --> 00:17:52,580
+في كمان مجموعة .. في كمان مجموعة .. في كمان مجموعة
+
+250
+00:17:52,580 --> 00:17:57,340
+اسمها micrococcus micrococcus يبقى micrococcus,
+
+251
+00:17:57,520 --> 00:18:01,740
+staphylococcus و streptococcus ما المشترك بين ال
+
+252
+00:18:01,740 --> 00:18:06,100
+staphylococcus و ال micrococcus الكتاليزي انهم ال
+
+253
+00:18:06,100 --> 00:18:09,580
+ground boaster كوكساي و الكتاليزي بوستر كبنا ميز
+
+254
+00:18:09,580 --> 00:18:12,320
+بينهم هذا الفحص اللي قدامكوا اسمه ال isostaphine
+
+255
+00:18:13,730 --> 00:18:17,230
+Lysostafin واضح من اسمه إنه بيعمل lice لمين؟
+
+256
+00:18:17,230 --> 00:18:22,510
+للاستاف، كل الاستاف بتدوب، كده إيش يعني بتدوب؟
+
+257
+00:18:22,510 --> 00:18:25,950
+يعني بتموت و بتتحلل، بتموت و بتتحلل، أصلا ال
+
+258
+00:18:25,950 --> 00:18:29,150
+Benzaline عارفين كيف بيقتل البكتيريا اللي بيقتل
+
+259
+00:18:29,150 --> 00:18:32,990
+الاستافين الجوكاساوريا برضه بتتحللأصلا اكتشافه
+
+260
+00:18:32,990 --> 00:18:37,470
+البنسيلين من قبل Alexander Fleming كان بطريقة هذه
+
+261
+00:18:37,470 --> 00:18:40,430
+فطر نمى جنب ال .. ال .. الـ cephalopoccus aureus
+
+262
+00:18:40,430 --> 00:18:45,150
+دول المستعمرات اللي أصلا كانت موجودة فالبكتيريا
+
+263
+00:18:45,150 --> 00:18:48,950
+ممكن إذا ال cell wall تبعها ات .. اتحلل و أنتوا
+
+264
+00:18:48,950 --> 00:18:51,550
+عارفين البنسيلين مش تعملان أيه؟ على ال cell wall
+
+265
+00:18:51,550 --> 00:18:54,930
+طب كيف بيناعرف ان ال .. ال .. اتحلل .. البكتيريا
+
+266
+00:18:54,930 --> 00:18:57,830
+ولا اتحللت ايش؟ طلع على انبوبة و هذا .. هذا يعني
+
+267
+00:18:57,830 --> 00:19:01,910
+نقطة كتير مهمة بنستخدمها احنافي ال comparisons
+
+268
+00:19:01,910 --> 00:19:09,230
+بنجيب مثلا ورقة مطبوعة تمام و بنحط أمبوبتين قدام
+
+269
+00:19:09,230 --> 00:19:14,730
+الورقة و بنقرأ الكلام المطبوع من خلال الأنابيب في
+
+270
+00:19:14,730 --> 00:19:17,470
+هذا الحالة ما بنقرأش احنا قاعدين هنا نقرأ وضوح
+
+271
+00:19:17,470 --> 00:19:21,350
+الخطوط فقط ما بنقرأش كلام نقرأ وضوح الخطوط مين
+
+272
+00:19:21,350 --> 00:19:26,830
+أوضح؟ واضح انه هنا الخط واضح جدا بينما هنا يعني ال
+
+273
+00:19:26,830 --> 00:19:29,370
+turbidity هنا أعلى من ال turbidity هنا
+
+274
+00:19:45,260 --> 00:19:50,300
+مؤشر على أن البكتيريا اللي نميها هنا لم تتحلل ماذا
+
+275
+00:19:50,300 --> 00:19:53,280
+يعني ذلك؟ انها resistant ماذا يعني ذلك؟
+
+276
+00:19:53,280 --> 00:20:00,680
+micrococcus فوق البكتيريا فقدت تماما من الأنبوبة
+
+277
+00:20:01,950 --> 00:20:06,190
+وبتالي هى sensitive وبتالي هى stiff وصلت الصورة
+
+278
+00:20:06,190 --> 00:20:10,490
+هذا فحص كتير مهم لانه بيميز لبن two organisms
+
+279
+00:20:10,490 --> 00:20:16,430
+بيشبهوا بعض بدرجة كبيرة او درجات الشبه غير ان هم
+
+280
+00:20:16,430 --> 00:20:19,590
+جرام بوستد كوكساي وكيتاليز بوستد كمان على الطبق
+
+281
+00:20:19,590 --> 00:20:24,050
+they may look similar يعني الاستافيلو كوكس
+
+282
+00:20:24,050 --> 00:20:28,070
+والمايكرو كوكس ممكن يكونوا أشكال مستعملات قريبة من
+
+283
+00:20:28,070 --> 00:20:33,810
+بعضهم البعض فواجب علينا ان نحن نميز بيننجي لـ ..
+
+284
+00:20:33,810 --> 00:20:40,290
+ال .. ال .. ال .. ال rapid tests في المعمل
+
+285
+00:20:40,290 --> 00:20:44,330
+هتشتغلوا اندول بأنبوبة و Methyl Red في أنبوبة و
+
+286
+00:20:44,330 --> 00:20:46,810
+Vagos Blitzkrieg في أنبوبة و Citrate في أنبوبة و
+
+287
+00:20:46,810 --> 00:20:50,450
+Motility في أنبوبة يعني لو بدي أعمل عشرين فحص أنا
+
+288
+00:20:50,450 --> 00:20:53,690
+بدي عشرين أنبوبة بديراك هالجدي و أنا بيب تقريبا
+
+289
+00:20:53,690 --> 00:20:58,970
+عشرة صندوق طولها أو أكتر يعني عشان أعمل مجموعة ..
+
+290
+00:20:58,970 --> 00:21:02,530
+م .. م .. فحصات لمجموعة بكتيريا بعب ال incubator
+
+291
+00:21:05,330 --> 00:21:09,890
+فالشركات لجأت إلى ما يسمى بال miniaturization يعني
+
+292
+00:21:09,890 --> 00:21:15,630
+لكبير نخليه لصغير زي ما بنعمل مجسمات لعمارة
+
+293
+00:21:15,630 --> 00:21:18,850
+الجدران احنا عملنا مجسمات لفحوطات صغيرة بس مجسمات
+
+294
+00:21:18,850 --> 00:21:24,270
+حقيقية و فعالة شغالة تمام شايفين الشريط هذا اللي
+
+295
+00:21:24,270 --> 00:21:28,810
+قدامنا اسمه ABI وانسوا الباقي ABI Analytical
+
+296
+00:21:28,810 --> 00:21:32,930
+Profile Index من شركة فرنسية مشهورة اسمها Biomero
+
+297
+00:21:34,600 --> 00:21:40,360
+entered مجموعة من ال strips هذه لمجموعات مختلفة من
+
+298
+00:21:40,360 --> 00:21:45,440
+البكتيري يعني مثلا عنا ABI-20E للانتروبكترياسي
+
+299
+00:21:45,440 --> 00:21:50,040
+والنانثارمينتر عنا ABI-staph للستافيرو كوكس عنا
+
+300
+00:21:50,040 --> 00:21:54,340
+ABI-strip للستريبتو كوكس عنا ABI-anaerobes
+
+301
+00:21:54,340 --> 00:21:58,260
+للبكتيرى اللى هوائية عنا ABI-nyseria تبقى فينا
+
+302
+00:21:58,260 --> 00:22:03,270
+أنواع مختلفة من ايه؟ من ال ABI ال ABI هو مسمىللـ
+
+303
+00:22:03,270 --> 00:22:07,890
+system نفسه لكن ال system موزع على أنواع مختلفة من
+
+304
+00:22:07,890 --> 00:22:11,810
+اللي هو البكتيريا طيب يالا بدنا نحفظ ال positive و
+
+305
+00:22:11,810 --> 00:22:17,990
+ال negative تبعات هذولة بتقدروا تحفظهموا؟ تقدروا؟
+
+306
+00:22:17,990 --> 00:22:23,170
+طيب لما أجيبلكوا كمان 200 .. 200 .. 200 بكتيريا ب
+
+307
+00:22:23,170 --> 00:22:28,650
+20 فحص هتكون العملية يعني معقدة فانا مضطر أن ألجأ
+
+308
+00:22:28,650 --> 00:22:30,650
+إلى ال internet قرر أن يكون هناك internet
+
+309
+00:22:34,650 --> 00:22:41,530
+القاعة في برنامج اسمه ID backed سجله اسمه ID
+
+310
+00:22:41,530 --> 00:22:42,090
+backed
+
+311
+00:22:48,000 --> 00:22:53,960
+الفايل اللي مش موجود انا هنزلكه نسخة على الفيسبوك
+
+312
+00:22:53,960 --> 00:22:57,520
+لكن عشان تقدر تنزله على ويندوز حديث هذا المصمم كان
+
+313
+00:22:57,520 --> 00:23:01,600
+لويندوز اكس بي لازم تتطلعوا على اليوتيوب هذا عشان
+
+314
+00:23:01,600 --> 00:23:05,700
+تقدروا تعرفوا كيف بنعمل عملي التوافق للبرنامج وكيف
+
+315
+00:23:05,700 --> 00:23:07,140
+بنشغله هذا انا قعدت
+
+316
+00:23:18,290 --> 00:23:23,850
+هو المفروض انه بنزل بكل سهولة من الانترنت بعد
+
+317
+00:23:23,850 --> 00:23:24,330
+الشكل
+
+318
+00:23:32,000 --> 00:23:35,380
+عادة من كلكم قبولها بتنزيل الملفات وتشغيلها أو
+
+319
+00:23:35,380 --> 00:23:38,580
+تعمل set up يعني next next مافيش أي مشكلة في
+
+320
+00:23:38,580 --> 00:23:41,680
+الموضوع بس تعرفون بتخزنوا الملف تبعكم و بعدين
+
+321
+00:23:41,680 --> 00:23:44,720
+تعملوا set up المشكلة في set up في برامج من النوع
+
+322
+00:23:44,720 --> 00:23:50,840
+هذا الدعم الفني توقف عند ال xp و التالي بتدعمش
+
+323
+00:23:50,840 --> 00:23:55,440
+برامج اللي هم ال .. ال .. ال vista و ال seven و ال
+
+324
+00:23:55,440 --> 00:23:58,520
+eight في ال windows عشان تقدر تشتغل عليها لازم
+
+325
+00:23:58,520 --> 00:24:00,660
+نعمل حركة الحين بنشوفها كمان شوية
+
+326
+00:24:04,090 --> 00:24:08,310
+هنا بس عاملا بنختار ال location لتنزيل الملف ملف
+
+327
+00:24:08,310 --> 00:24:24,990
+صغير جدا لا يستغل ثواني في التحميل ناخده
+
+328
+00:24:24,990 --> 00:24:28,870
+نحطه على ال D هاي البرنامج
+
+329
+00:24:39,130 --> 00:24:47,550
+استكشاف مشاكل التوافق واصلاحها او خصائص تشوفت
+
+330
+00:24:47,550 --> 00:24:55,370
+التوافق؟ نختار تشغيل في وضع التوافق على ويندوز
+
+331
+00:24:55,370 --> 00:24:59,710
+جديد مثل ويندوز اكس
+
+332
+00:24:59,710 --> 00:25:05,570
+بي توإذا ماعملش الحركة دى مش هيشتغل البرنامج مش
+
+333
+00:25:05,570 --> 00:25:12,350
+هيشتغل البرنامج الآن خلاص هو جاهز للتشغيل بنشغله
+
+334
+00:25:12,350 --> 00:25:18,850
+كمسئول ك administrator يعني
+
+335
+00:25:18,850 --> 00:25:24,970
+هاي
+
+336
+00:25:24,970 --> 00:25:28,690
+set up زى ما حكالكوا next next مافيش حاجة special
+
+337
+00:25:28,690 --> 00:25:34,390
+في الموضوعو خلال ثانية واحدة تقريبا بيكتمل لغتات
+
+338
+00:25:34,390 --> 00:25:43,070
+تبعته هاي
+
+339
+00:25:43,070 --> 00:25:50,910
+و الآن صار في قائمة البرنامج طبعا
+
+340
+00:25:50,910 --> 00:25:54,890
+البرنامج فيه إمكانيتين فيه إمكانية استخدام التعرف
+
+341
+00:25:54,890 --> 00:25:56,190
+قائمة التعرف
+
+342
+00:26:00,040 --> 00:26:04,480
+و فيه هو أصلا databases قواعد بيانات مجهزة و مجهزة
+
+343
+00:26:04,480 --> 00:26:09,100
+زي ال ABI 10 ال ABI 10 إذا بنفتح بهذه الطريقة
+
+344
+00:26:09,100 --> 00:26:12,880
+بيعطينا boxes شايفين الصناديق هذه بنعبيها ال
+
+345
+00:26:12,880 --> 00:26:14,980
+positive و ال negative تبعنا اللي احنا حصلنا عليه
+
+346
+00:26:14,980 --> 00:26:17,820
+في المعمل من ال strip positive negative negative
+
+347
+00:26:17,820 --> 00:26:20,940
+positive حسب النتائج اللي طلعت معاناوبضغطة واحدة
+
+348
+00:26:20,940 --> 00:26:25,020
+enter بيعطينا اسم ال microbe ويبقونا جداش نسبة
+
+349
+00:26:25,020 --> 00:26:30,900
+التعرف بدقة على هذا ال microbe مهم جدا انه نعرف
+
+350
+00:26:30,900 --> 00:26:35,560
+كيف نتعامل مع هذه البرامج لإنها جزء من المستقبل
+
+351
+00:26:35,560 --> 00:26:41,680
+تبعنا في ال microbe لحظة ال green واصل part
+
+352
+00:26:41,680 --> 00:26:44,760
+لتقريبا 99% فبقى انا احكي ال identification تبعت
+
+353
+00:26:44,760 --> 00:26:49,280
+هي قوية ومعرفة على انها vibrio choleraباقي انا
+
+354
+00:26:49,280 --> 00:26:55,580
+اذكر كلمة واحدة هذه أجهزة برامج غبيه سريعه احنا
+
+355
+00:26:55,580 --> 00:26:57,680
+اللي بنلقنها نعم احنا اللي بنعطيها المعلومات
+
+356
+00:26:57,680 --> 00:27:02,820
+وبترجلنا بس بترجلناها بسرعة فإذا انت شغل غلطو
+
+357
+00:27:02,820 --> 00:27:05,860
+اعطته اي حاجة انا دخلت من عندى positive negative
+
+358
+00:27:05,860 --> 00:27:09,020
+positive و اعطانى اجابة عسب قواعد البيانات اللى
+
+359
+00:27:09,020 --> 00:27:12,760
+عنده فلازم نشتغل صح عشان لما ادخل صح يعطينا هو
+
+360
+00:27:12,760 --> 00:27:17,420
+برضه ايضا تعريف صح هذا احد البرامج اللى اسمه بنا
+
+361
+00:27:17,420 --> 00:27:23,300
+ID بقى سهل جدا فى التعامل معاه ويمكن اضافة قواعد
+
+362
+00:27:23,300 --> 00:27:27,020
+بيانات من عندنا احنا نعمل قواعد بيانات خاصة فينا
+
+363
+00:27:27,020 --> 00:27:30,100
+وندخلها يعني لو انا اتفهم ان انا اتطورت system
+
+364
+00:27:30,100 --> 00:27:34,740
+جديدمش ال ABI-20 عملت 10 فحصات بدل 20 فحص سبعة
+
+365
+00:27:34,740 --> 00:27:38,520
+فحصات بدل 20 فحص ممكن أدخلهم أنا أعمل قاعدة بيانات
+
+366
+00:27:38,520 --> 00:27:42,100
+و أدخلهم وصيلة استخدامها في التعرف على ال micro
+
+367
+00:27:42,100 --> 00:27:48,420
+organism طيب نشوف اللي .. اللي قلنا عنه ال PEP1
+
+368
+00:27:48,420 --> 00:27:54,580
+أنا
+
+369
+00:27:54,580 --> 00:27:58,640
+بحب دايما أضيف كلمة bacteria عشان مايجيبوش هدا
+
+370
+00:27:58,640 --> 00:27:59,960
+التانية غير متوقعة
+
+371
+00:28:03,160 --> 00:28:06,480
+بروبولاستيك هو اختصار البروبولاستيك identification
+
+372
+00:28:06,480 --> 00:28:07,960
+of bacteria for windows
+
+373
+00:28:16,120 --> 00:28:19,740
+من اجمل .. من اجمل برامج لل .. لل researchers مش
+
+374
+00:28:19,740 --> 00:28:22,700
+ال practical صحيح لل .. لل .. لل عاملين لكن اللي
+
+375
+00:28:22,700 --> 00:28:25,880
+بيحب يتعرف على الميكروبات المجهولة من اي source من
+
+376
+00:28:25,880 --> 00:28:28,920
+اي مصدر سواء بيئي او طبية و كده بيستخدم هذا
+
+377
+00:28:28,920 --> 00:28:31,920
+البرامج انا بانصح في طلابي الملستير اللي بيستخدموا
+
+378
+00:28:31,920 --> 00:28:37,100
+بكتيريا غير .. او مجهولة هم مش عارفينها يعني
+
+379
+00:28:37,100 --> 00:28:40,720
+بيدوروا على خاصية فيها مش على اسمها و بدهم لما
+
+380
+00:28:40,720 --> 00:28:45,740
+لاقوا الخاصية يتعرفوا على الاسم بعد ذلك فممكن ناخد
+
+381
+00:28:46,380 --> 00:28:50,100
+نعمل download و ندور على المصفوفات اللي هي قواعد
+
+382
+00:28:50,100 --> 00:28:53,660
+البيانات و عنده قائمة محترمة جدا من المصفوفات كلها
+
+383
+00:28:53,660 --> 00:28:58,420
+for free يعني برنامج for free بيساعدنا في التعرف
+
+384
+00:28:58,420 --> 00:29:02,170
+على ال micro organismهنا اللي خدعناه ان نتكلم عن
+
+385
+00:29:02,170 --> 00:29:05,370
+البرامج اللي مستخدمة في التعرف على البكتيريا هو
+
+386
+00:29:05,370 --> 00:29:10,330
+وجود أنظمة زي ال ABI صحيح سهلت علينا لكن في نفس
+
+387
+00:29:10,330 --> 00:29:14,690
+الوجود ذات اللي على الذاكرة البشرية وبتالي اضطررنا
+
+388
+00:29:14,690 --> 00:29:18,830
+نلجأ للبرامج المحاوظبة اللي بتساعدنا بشكل كبير في
+
+389
+00:29:18,830 --> 00:29:20,770
+التعرف على هذه الميكروفر
+
+390
+00:29:25,460 --> 00:29:28,120
+أحنا يعني طول الفترة السابقة أتكلم على
+
+391
+00:29:28,120 --> 00:29:31,340
+Staphylococcus aureus المكورة الأنقضية الذهبية
+
+392
+00:29:31,340 --> 00:29:37,920
+إلها أخوات مهمين زي Staphylococcus epidermidis و
+
+393
+00:29:37,920 --> 00:29:44,420
+Staphylococcus saprophytic لكن اللي بدأ أتكلم عنه
+
+394
+00:29:44,420 --> 00:29:50,340
+الآن أنه بكفي أنه نخدم حق ل Staphylococcus
+
+395
+00:29:50,340 --> 00:29:54,680
+epidermidis و Staphylococcus saprophyticكنا نقول
+
+396
+00:29:54,680 --> 00:29:57,900
+إن الأورياس هي البعثوجين الوحيد في كل
+
+397
+00:29:57,900 --> 00:30:01,860
+Staphylococcus بعد فترة كنا لأ الأورياس ال .. ال
+
+398
+00:30:01,860 --> 00:30:05,260
+Epidermis بتسبب Endocarditis و بتسبب التهيبات ممكن
+
+399
+00:30:05,260 --> 00:30:09,060
+في صممات القلب السابروفايتكاس اللي جاية التهياب
+
+400
+00:30:09,060 --> 00:30:11,800
+منه سيستايتس اللي هي التهياب المثاني التهياب
+
+401
+00:30:11,800 --> 00:30:16,100
+المثاني طب الآن لو دورته على جوجل و كتبته
+
+402
+00:30:16,100 --> 00:30:19,200
+Coagulase Negative Staphylococcus و مين بينتمي على
+
+403
+00:30:19,200 --> 00:30:22,080
+Coagulase Negative Staphylococcus؟ التنتهي، مصبوف؟
+
+404
+00:30:23,270 --> 00:30:28,330
+هتلاجوا أنه وصلوا مراتب في بعض الأحيان أعلى من الـ
+
+405
+00:30:28,330 --> 00:30:31,370
+Staphylococcus aureus في نسبة احداثهم للأمراض
+
+406
+00:30:31,370 --> 00:30:37,850
+المستشفيةالـ Coagulase Negative Staphylococci نظرة
+
+407
+00:30:37,850 --> 00:30:40,350
+مختلفة عن السابق يعني الـ Coagulase Negative
+
+408
+00:30:40,350 --> 00:30:43,550
+Staphylococci أصبحت تنافس الـ Coagulase Positive
+
+409
+00:30:43,550 --> 00:30:46,910
+Staphylococci وأنا بقولكوا أكتر من دراسة منها في
+
+410
+00:30:46,910 --> 00:30:50,050
+غزهان اللي جيناه بالفعل في بعض الأقسام في
+
+411
+00:30:50,050 --> 00:30:53,610
+المستشفيات الـ Coagulase Negative Staphylococci
+
+412
+00:30:53,610 --> 00:30:57,090
+كانت أعلى من اللي هو الـ Staphylococcus aureus فهي
+
+413
+00:30:57,090 --> 00:31:03,030
+في صعودوالصعود تبعها ناجم عن إنه هي تمتلك مؤهلات
+
+414
+00:31:03,030 --> 00:31:07,470
+وزادت مؤهلاتها أكتر من أحصار ال resistance لل
+
+415
+00:31:07,470 --> 00:31:10,810
+antibiotic وبالتالي انتشارها في المستشفيات بيكون
+
+416
+00:31:10,810 --> 00:31:14,730
+واسع أنطق Major component اسمها Epidermis على
+
+417
+00:31:14,730 --> 00:31:17,450
+الجذب موجودة فهي major component of skin flora
+
+418
+00:31:17,450 --> 00:31:21,870
+opportunistic infection لكن ال opportunistic كانت
+
+419
+00:31:21,870 --> 00:31:26,870
+بتزيد تذكروا دايما المستشفى بيه ألمين؟ للمرضى
+
+420
+00:31:26,870 --> 00:31:30,270
+للمرضى يعني لمين؟ده اللي ال immune system عندهم
+
+421
+00:31:30,270 --> 00:31:33,750
+immunocompromised وبالتالي الميكروبات ال
+
+422
+00:31:33,750 --> 00:31:36,610
+opportunistic قادر ان يتعمل infection عند هذا
+
+423
+00:31:36,610 --> 00:31:39,770
+الناس ال heart valves وقلنا فيها ان ال
+
+424
+00:31:39,770 --> 00:31:42,310
+endocarditis يعني من الأمراض المشهورة لهذه
+
+425
+00:31:42,310 --> 00:31:45,790
+البكتيريا ال identification ما بتعملاش يملحظ على
+
+426
+00:31:45,790 --> 00:31:49,530
+blood agar does not ferment manitol non pigmented
+
+427
+00:31:49,530 --> 00:31:55,680
+و QMAs طبعا نجات لحظوا النقاطمالكتوبرت فيرماتيشي
+
+428
+00:31:55,680 --> 00:31:59,000
+نيجاتيف و Coagulase نيجاتيف في كمان نقطة تالتة
+
+429
+00:31:59,000 --> 00:32:03,920
+DNA's نيجاتيف DNA's نيجاتيف لإن ما تلاقى ال
+
+430
+00:32:03,920 --> 00:32:09,640
+Staphylococcus aureus التلاتة بسيطة نيجي للميكروب
+
+431
+00:32:09,640 --> 00:32:13,040
+التاني اللي هو ال Sabrophytic و ال Sabrophytic
+
+432
+00:32:13,040 --> 00:32:17,400
+الصحيح يعني إرتباطه من لحد الآن في نوع واحد من
+
+433
+00:32:17,400 --> 00:32:20,160
+الأمراض اللي هو ال adrenaline tract infection و
+
+434
+00:32:20,160 --> 00:32:25,700
+بالأخرى ال cystitis اللي هي التهاب المثالطبعا من
+
+435
+00:32:25,700 --> 00:32:30,680
+ميزاته انه هو stuff كل صفات ال stuff عنده ما عدا
+
+436
+00:32:30,680 --> 00:32:34,680
+ال coagulase نجاتف طبعا و ال menitol fermentation
+
+437
+00:32:34,680 --> 00:32:39,380
+نجاتف ايضا و ال DNA نجاتفطب هذا بيصير هو و ال
+
+438
+00:32:39,380 --> 00:32:42,460
+Staphylococcus epidermidis في نفس الخانة كيبنا
+
+439
+00:32:42,460 --> 00:32:47,600
+ملزم عن بعض بيفحص اسمه Novobiocin ال Novobiocin هو
+
+440
+00:32:47,600 --> 00:32:49,880
+يبقى عن ال antibiotic يعني يبقى عن ال antibiotic
+
+441
+00:32:49,880 --> 00:32:53,640
+بنستخدمه في ال differentiation between saprophytic
+
+442
+00:32:53,640 --> 00:32:59,820
+acid resistant و ال epidermis بتكون sensitive طبعا
+
+443
+00:32:59,820 --> 00:33:03,870
+الصورة اللي قدامنا نعرض الصورةبتوضح ان القُرص
+
+444
+00:33:03,870 --> 00:33:07,050
+الانتيباتيكي اللي هو نوغروفايوسين و حواليه zone of
+
+445
+00:33:07,050 --> 00:33:10,650
+inhibition منطقة خالية من النمو ده لإنه البكتيريا
+
+446
+00:33:10,650 --> 00:33:14,230
+اللي هنا sensitive بينما في الجزء التاني من الطبق
+
+447
+00:33:14,230 --> 00:33:18,130
+مافيش zone of inhibition وبالتالي البكتيريا resist
+
+448
+00:33:18,130 --> 00:33:21,670
+طيب
+
+449
+00:33:21,670 --> 00:33:24,490
+نيجي لجزء التاني من الموضوع اللي هو
+
+450
+00:33:28,700 --> 00:33:32,860
+بنطلقها على تنتين في أكتر من السلاسل يعني لو كانوا
+
+451
+00:33:32,860 --> 00:33:40,340
+تنتين برضه بنسميهم streptococci صور
+
+452
+00:33:40,340 --> 00:33:43,100
+توضحية هذا الصور اللي بيقولنا صورة توضحية مش صورة
+
+453
+00:33:43,100 --> 00:33:46,280
+حقيقية هي عبارة عن رسم من تحت ال microscope لكن
+
+454
+00:33:46,280 --> 00:33:51,980
+بتوضح لنا إن ال chains طولها
+
+455
+00:33:51,980 --> 00:33:56,080
+مختلف قد تكون مكوّنة من تنتين بالفعلو هذا حسب ال
+
+456
+00:33:56,080 --> 00:34:00,160
+handling ال specimen يعني لو أنا أخدت أنبوبة و
+
+457
+00:34:00,160 --> 00:34:06,740
+زرعت فيها stirptococcus by genes أنبوبة liquid و
+
+458
+00:34:06,740 --> 00:34:11,820
+ماعملتلاش أي disturbance ماحركتاش خالص بعد 24 ساعة
+
+459
+00:34:11,820 --> 00:34:16,820
+لو يطلعت عليها بيلاجيها عملة النمو تبعها يشبه ما
+
+460
+00:34:16,820 --> 00:34:19,680
+يسمى بال cotton ball cotton ball يعني زي كرات
+
+461
+00:34:19,680 --> 00:34:25,590
+بالقطنكورة القطن من أيش كورة القطن؟ تخيلوا سلسلة
+
+462
+00:34:25,590 --> 00:34:30,810
+جالة تطول، إيه بتطول؟ في الآخر بتبقى لها مسكة حلوة
+
+463
+00:34:30,810 --> 00:34:36,830
+لك و لا تنحني؟ تنحني فبصير تلف و تشربك بع بعضها و
+
+464
+00:34:36,830 --> 00:34:40,390
+بتعملي زي إيه؟ زي الكورة، لو أخدت قطرة بهدوء من
+
+465
+00:34:40,390 --> 00:34:44,990
+أوحة تحت الميجروسكوب هلاجي very long .. very long
+
+466
+00:34:44,990 --> 00:34:49,780
+chainsيبقى طول السلسلة معتمدة على وين نمت
+
+467
+00:34:49,780 --> 00:34:52,600
+البكتيريا، إيش الظروف اللي اتعرضت إلها، وعملتي ال
+
+468
+00:34:52,600 --> 00:34:56,260
+handling، ال handling اللي هي و أنا بضرع فيها، أو
+
+469
+00:34:56,260 --> 00:34:58,220
+عفوا مش بضرع فيها، بعمل general staining، مش بعمل
+
+470
+00:34:58,220 --> 00:35:01,720
+mixing أنا، ال mixing هذا هو برضه بيكسر اللي هو ال
+
+471
+00:35:01,720 --> 00:35:07,240
+.. ال .. البكتيريامذكركم بال beta alpha gamma
+
+472
+00:35:07,240 --> 00:35:10,620
+hemolysis احنا في الاستخدام الكوكاس اولى استخدامنا
+
+473
+00:35:10,620 --> 00:35:12,900
+صفة ال beta hemolysis مرة واحدة اذا كنا beta
+
+474
+00:35:12,900 --> 00:35:16,480
+hemolytic او non-beta hemolytic بس بدكم صحيح ال
+
+475
+00:35:16,480 --> 00:35:21,740
+hemolysis اكتر استخدام له في الstriptococciيعني
+
+476
+00:35:21,740 --> 00:35:27,080
+أقولكوا كمان تعبير أدق قد نصف البكتيريا في الـ
+
+477
+00:35:27,080 --> 00:35:29,700
+streptococcus بالـ hemolysis تبعها، عفاش ما بنقول
+
+478
+00:35:29,700 --> 00:35:33,040
+Alpha hemolytic streptococci Beta hemolytic
+
+479
+00:35:33,040 --> 00:35:38,160
+streptococci Gamma hemolytic streptococci يبقى
+
+480
+00:35:38,160 --> 00:35:41,480
+عملية تصنيف الـ streptococci دخل فيها مين؟ الـ
+
+481
+00:35:41,480 --> 00:35:43,640
+hemolysis على الـ blood vessels، يعني ده فيها صفة
+
+482
+00:35:43,640 --> 00:35:48,380
+من الصفات المهمة جدا في الميكروبيل دي أرفك و تدفأ
+
+483
+00:35:48,380 --> 00:35:51,440
+نقلب، و هذه الصفة شاركت مع مين؟مع ال
+
+484
+00:35:51,440 --> 00:35:54,540
+Staphylococcus Gram-positive chains or pairs
+
+485
+00:35:54,540 --> 00:35:59,840
+كتاليز نيجاتيف وبين بسين Staphylococci are كتاليز
+
+486
+00:35:59,840 --> 00:36:05,140
+بوسطف مش معنى ال Staphylococci وهي facultative عن
+
+487
+00:36:05,140 --> 00:36:10,600
+أيروب كتاليز بوسطف ليش ال Stryptococci ما تكون
+
+488
+00:36:10,600 --> 00:36:12,960
+زيها كتاليز بوسطف ما هي facultative عن أيروب و
+
+489
+00:36:12,960 --> 00:36:17,940
+بتتحمل وجود ال oxygenبقول إن النظام الموجود فيها
+
+490
+00:36:17,940 --> 00:36:22,280
+بيختلف عن النظام الموجود في الـ Staphylococci في
+
+491
+00:36:22,280 --> 00:36:26,500
+الـ Staphylococci فيها SOD .. إيش ال SOD؟ Super
+
+492
+00:36:26,500 --> 00:36:31,620
+Oxide Desmutase زائد كيتاليز بينما في ال
+
+493
+00:36:31,620 --> 00:36:36,740
+Striptococci فيها SOD زائد Peroxidase خدوا بالكوا
+
+494
+00:36:37,110 --> 00:36:41,950
+البروكسديس والكتاليز يقوموا بنفس الوظيفة تحويل ال
+
+495
+00:36:41,950 --> 00:36:48,530
+H2O2 إلى ميه زائد أكسجين بس في الكتاليز الأكسجين
+
+496
+00:36:48,530 --> 00:36:53,810
+بيطلع كموليكلر أكسجينفي البروكسيدايز الأكسجين
+
+497
+00:36:53,810 --> 00:36:57,670
+بيستخدم مباشرة في ال reduction لمركبات تانية
+
+498
+00:36:57,670 --> 00:37:00,990
+فبيظهرش على أنه molecular أكسجين بجانب الفحص لو
+
+499
+00:37:00,990 --> 00:37:05,230
+حطينا بكتيريا عندها بيروكسيدايز على هيدروجين
+
+500
+00:37:05,230 --> 00:37:09,330
+بيروكسايد هل هيطلع bubbles؟ مش هيطلع bubbles لأن
+
+501
+00:37:09,330 --> 00:37:12,310
+مافيش فيها كتاليز فيها بيروكسيدايز
+
+502
+00:37:15,510 --> 00:37:18,210
+اللوحة الصعبة اللي هنوجب في عندها محاضرتنا في
+
+503
+00:37:18,210 --> 00:37:21,790
+الآخر لانه مش هنقدر نخلصها في المحاضرة في الوقت
+
+504
+00:37:21,790 --> 00:37:25,570
+المتبقى فانديها اسمها Stryptomycin و ال
+
+505
+00:37:25,570 --> 00:37:30,030
+Stryptomycin two types مكتوب عليهم واحد
+
+506
+00:37:30,030 --> 00:37:34,350
+Stryptomycin S واحد اسمه Stryptomycin O تعالوا
+
+507
+00:37:34,350 --> 00:37:38,070
+نطلع مع بعض على الصفورة و نشوف ايش يعني
+
+508
+00:37:38,070 --> 00:37:38,990
+Stryptomycin
+
+509
+00:37:45,050 --> 00:37:50,590
+O و Structurizing S
+
+510
+00:37:50,590 --> 00:37:55,490
+من أين جاءت أحرف هذه الكلمات من أين جاءت؟ تركزوا
+
+511
+00:37:55,490 --> 00:38:06,950
+معايا الاتنين لهم خاصية معينة تجاه الأكسجين هذا
+
+512
+00:38:06,950 --> 00:38:10,890
+الأول Oxygen Labia
+
+513
+00:38:13,400 --> 00:38:18,340
+يعني بيتدمر، بيتأثر بالـ Oxygen بينما التاني
+
+514
+00:38:18,340 --> 00:38:23,420
+Stable الاحرف
+
+515
+00:38:23,420 --> 00:38:30,140
+Oxygen ل Bi و هذه Oxygen A Stable طبعا التسمية
+
+516
+00:38:30,140 --> 00:38:35,780
+ممكن تكون مهمة، لكن برضه مش هي اللي المقصودة عنها
+
+517
+00:38:35,780 --> 00:38:41,860
+الـ Structural ICO لما
+
+518
+00:38:41,860 --> 00:38:45,230
+بدخل هذاالاسم الإنسان من ضمن البكتيريا اللي
+
+519
+00:38:45,230 --> 00:38:50,170
+بتنتجه، اسمنا بيكوّله Antibodies، إيش بنسميه
+
+520
+00:38:50,170 --> 00:38:56,110
+Antibodies؟ Antistreptolysin، او .. إذا
+
+521
+00:38:56,110 --> 00:39:00,030
+streptolysin هو مين؟ هو الاندجين، وال Antibody
+
+522
+00:39:00,030 --> 00:39:04,310
+اسمه Antistreptolysin، مين اللي بنتجه أساسا؟
+
+523
+00:39:09,130 --> 00:39:12,390
+The streptococcus biogenes number one cause of
+
+524
+00:39:12,390 --> 00:39:17,590
+bacterial tonsillitis فاكرين
+
+525
+00:39:17,590 --> 00:39:21,910
+تونسولايتس؟ فيه هدافيكو يعني هرب منها في يوم من
+
+526
+00:39:21,910 --> 00:39:26,210
+الأيام خاصة في سن الطفولة معظمنا وصيب بيها مرارا
+
+527
+00:39:26,210 --> 00:39:30,890
+وتكرارا مرارا وتكرارا يبجى بتوقع أنه دخل عندنا ميم
+
+528
+00:39:30,890 --> 00:39:36,960
+SO واتكون عندنا ASOيعني لو فحصناك و الأن كلكوا
+
+529
+00:39:36,960 --> 00:39:40,500
+هلاجة أنكوا ASO هذا لا يعني أنكوا الأن مصابين
+
+530
+00:39:40,500 --> 00:39:46,060
+بالاستخدام وبالتالي اضطرينا أن نلجأ إلى حيلة العد
+
+531
+00:39:46,060 --> 00:39:51,040
+أو قياس التركيز فضفنا حرف ال T في الآخر صار اسم
+
+532
+00:39:51,040 --> 00:39:57,540
+الفحص ASOT و T معناها title و title معناها
+
+533
+00:39:57,540 --> 00:40:02,340
+concentrationوبالتالي الفحص بطل يقول positive
+
+534
+00:40:02,340 --> 00:40:07,080
+نيجاتين صار يجيس يعني بيقيس كمية اللي هو ال
+
+535
+00:40:07,080 --> 00:40:10,900
+antibodies لل Streptolysin وبالتالي اسم الفحص الآن
+
+536
+00:40:10,900 --> 00:40:17,660
+اسمه Anti Streptolysin O طيب بيجيس تركيز هل هناك
+
+537
+00:40:17,660 --> 00:40:20,720
+علاقة بين التركيز وبين وجود إصابة حالية أو إصابة
+
+538
+00:40:20,720 --> 00:40:25,620
+سابقة نعم يوجد إذا كان إصابة سابقة غالبا التركيز
+
+539
+00:40:25,620 --> 00:40:32,110
+بيكون أقل من 200 انتقاش نجنة per mlإذا كان إصابة
+
+540
+00:40:32,110 --> 00:40:40,330
+حالية غالبا فيكون أكتر من 200 يبقى الآن صراحة نعرف
+
+541
+00:40:40,330 --> 00:40:44,130
+الميز ما بين إصابة حالية وإصابة صادفة لإنه كان
+
+542
+00:40:44,130 --> 00:40:49,530
+بالأول صعب صعب جدا علينا نعرف لإن كل الناس أو معظم
+
+543
+00:40:49,530 --> 00:40:53,030
+الناس أصيبوا في يوم من الأيام بالستيكوجاست
+
+544
+00:40:54,620 --> 00:40:57,560
+ووصف واضحة الصورة هذه أن الـ Antistryptol هو ..
+
+545
+00:40:57,560 --> 00:40:59,640
+واخدوا بالكم من أحد البروحانات المشهورة جدا في
+
+546
+00:40:59,640 --> 00:41:03,740
+قطاع غزة للتعارف على أن مسبب اللواز هو
+
+547
+00:41:03,740 --> 00:41:08,740
+Stryptococcus Bionica كمان شغل مهم جدا أنا قولت
+
+548
+00:41:08,740 --> 00:41:13,880
+فيه تايتر، صح؟ التايتر لما بنجيسه، احنا بنعطيه في
+
+549
+00:41:13,880 --> 00:41:18,300
+التقرير للطبيب، بنعطي التقرير، بنقول أن التايتر
+
+550
+00:41:18,300 --> 00:41:23,280
+800 مثلا، هو 200، 400، 800، 1600، اللي هو cereal
+
+551
+00:41:23,280 --> 00:41:27,780
+dilutionفكنا له 800 مثلا الطبيب هنا لازم ياخد باله
+
+552
+00:41:27,780 --> 00:41:31,360
+من انه ال concentration عالية والاسبوع الماضي
+
+553
+00:41:31,360 --> 00:41:37,220
+تذكروا تحدثنا عن بعض الأمراض المرتبطة بال immunity
+
+554
+00:41:37,220 --> 00:41:43,500
+بمعنى آخر ان الميكروبات بتنتج أنتجينات شبيهة
+
+555
+00:41:43,500 --> 00:41:48,040
+بأنتجناتناال antibodies اللي تنتج مش بس بتهاجم
+
+556
+00:41:48,040 --> 00:41:52,440
+البكتيريا بتهاجم برضه و من ضمن أسوأ الميكروفات
+
+557
+00:41:52,440 --> 00:41:55,820
+اللي هي ال streptococcus بيو جينز تمام؟ وبالتالي
+
+558
+00:41:55,820 --> 00:41:59,000
+لما بيلاقي title عالي يبقى هذا المرض بيكون under
+
+559
+00:41:59,000 --> 00:42:02,880
+observation طيلة فترة للعلاج حتى يمثل مع ال
+
+560
+00:42:02,880 --> 00:42:07,560
+complications اللي احنا تحدثنا عنها فحص مهم فحص
+
+561
+00:42:07,560 --> 00:42:11,060
+شائع عرفنا اليوم المصدر تبعه اللي هو ال
+
+562
+00:42:11,060 --> 00:42:15,630
+streptolysinواللي هو الخاص بمين بالـ streptococcus
+
+563
+00:42:15,630 --> 00:42:21,450
+biogenes طيب فينا اللي هو ال .. ال .. ال biogenic
+
+564
+00:42:21,450 --> 00:42:24,170
+exotoxins وهو عبارة عن erythrogenic كلمة
+
+565
+00:42:24,170 --> 00:42:26,450
+erythrogenic يعني واضح من انه .. انه بي .. بي ..
+
+566
+00:42:26,450 --> 00:42:30,210
+بيستهدف مين اللي هو بيستهدف ال .. ال red blood
+
+567
+00:42:30,210 --> 00:42:34,190
+cell ال hyaluronidase اللي قلنا بيفكفك الخلايا عن
+
+568
+00:42:34,190 --> 00:42:40,390
+بعضها البعض ال streptokinase ماروا معانا؟ kinases
+
+569
+00:42:40,390 --> 00:42:41,970
+عكس مين؟
+
+570
+00:42:44,690 --> 00:42:47,830
+عكس الكواغوليس، عكس الكواغوليس، قولنا الكواغوليس
+
+571
+00:42:47,830 --> 00:42:52,370
+ده ما بيحول الفيبرونيجن إلى فيبرين، هذا بيحول
+
+572
+00:42:52,370 --> 00:42:55,750
+الفيبرونيجن أو بيبام ال lysis لفيبرونيجن، بيبام ال
+
+573
+00:42:55,750 --> 00:42:59,590
+lysis لعفوة لما الفيبرين نقلت، وبالتالي بعيق عملية
+
+574
+00:42:59,590 --> 00:43:02,650
+مين قولنا؟ عملية ال healing للجروح، يعني الجروح ما
+
+575
+00:43:02,650 --> 00:43:07,620
+بتشفى، ما بتتكون جلطةالان البكتيريا موجودة و بتنتج
+
+576
+00:43:07,620 --> 00:43:11,260
+streptokinase و قولنالكوا أصبح streptokinase هذا
+
+577
+00:43:11,260 --> 00:43:16,420
+بالظبط سلعة مهمة دوائية تستخدم في علاج الجذبات
+
+578
+00:43:16,420 --> 00:43:23,260
+للمرضى اللي بيصير عندهم اللي هو الجذبات في ال
+
+579
+00:43:23,260 --> 00:43:27,020
+streptodoranase وهو عبارة عن deoxyribonuclease
+
+580
+00:43:27,020 --> 00:43:29,980
+يعني على غرار اللي هو ال dianase تبع ال
+
+581
+00:43:29,980 --> 00:43:35,060
+staphylococcus streptodoranase اسمهوطبعا هذا برضه
+
+582
+00:43:35,060 --> 00:43:42,020
+له أهمية في عملية الحصول أو تكسير المادة
+
+583
+00:43:42,020 --> 00:43:44,820
+النووية وبتاع الحصول عليها القواعد كغذاء
+
+584
+00:43:44,820 --> 00:43:52,420
+لالبكتيريا ف kill in proteinالـ M-protein المفروض
+
+585
+00:43:52,420 --> 00:43:55,700
+تكون فاكرينه الأصناع من ضمن من ضمن ال virulence من
+
+586
+00:43:55,700 --> 00:44:00,820
+ضمن ال virulence factors ويقولنا عنه antifagocytic
+
+587
+00:44:00,820 --> 00:44:06,740
+antifagocytic يعني بيمنع الفاجو سيتوسز وبالتالي هو
+
+588
+00:44:06,740 --> 00:44:11,760
+وسيلة جيدة من قبل البكتيريا لي حماية نفسها من اللي
+
+589
+00:44:11,760 --> 00:44:16,240
+حماية الفاجو سيتوسز تذكروا بتكلم عن biogenic
+
+590
+00:44:16,240 --> 00:44:20,900
+infection biogenic infection يعني ال bus ايش ال
+
+591
+00:44:20,900 --> 00:44:21,040
+bus
+
+592
+00:44:26,430 --> 00:44:32,870
+النترو فيلز هي الفاجو سايتس فبالتالي بيعطي قدرة
+
+593
+00:44:32,870 --> 00:44:38,150
+للبكتيريا انه يقتل الفاجو سايتس مش الفاجو سايتس هي
+
+594
+00:44:38,150 --> 00:44:42,990
+تقتل البكتيريا لانه بيهرب من الفاجو سايتس طبعا
+
+595
+00:44:42,990 --> 00:44:47,810
+غالبا ال immunity اللي بتنتج انها بتكون نتجة ضد
+
+596
+00:44:47,810 --> 00:44:51,970
+مين ضد اللي هو ال protein M يعني لو واحد جسمه كوّن
+
+597
+00:44:51,970 --> 00:44:54,790
+أجسام مضطرة ل protein M تمام هي اللي بتعطيه
+
+598
+00:44:54,790 --> 00:44:59,450
+immunityبرضه هو مسئول عن ال strain variation يعني
+
+599
+00:44:59,450 --> 00:45:03,210
+البكتيريا ممكن تغير ال protein M تبعها وبالتالي
+
+600
+00:45:03,210 --> 00:45:09,370
+تدخل في protein M1 و بعدين M2 M3 M4 وبالتالي
+
+601
+00:45:09,370 --> 00:45:15,030
+بعطيها هي ما يسمى ب strain variation تنوع السلالات
+
+602
+00:45:15,030 --> 00:45:21,810
+فكل مرة بيدخل الميكروب ب strain جديد أنا عندي ل M1
+
+603
+00:45:21,810 --> 00:45:27,850
+هو دخل ل M2 من ف M1 من ف M1 صح؟رقم تلاتة ال
+
+604
+00:45:27,850 --> 00:45:31,410
+reinfection هو المسئول عن ال reinfection مدام هو
+
+605
+00:45:31,410 --> 00:45:34,070
+اللي بيعمل strain variation المفروض ان انا وصاب
+
+606
+00:45:34,070 --> 00:45:38,070
+مرة واحدة و يتكوّل عندي مناعة تمام و انت هالموضوع
+
+607
+00:45:38,070 --> 00:45:42,630
+لكن انا وصاب مرة تلو المرة بسبب ورود M protein
+
+608
+00:45:42,630 --> 00:45:46,310
+لانه أخلف ال strains و هو بتاعي سهل عاملت بيه ال
+
+609
+00:45:46,310 --> 00:45:50,910
+reinfectionكابسول برضه في بعض ال .. ال .. ال ..
+
+610
+00:45:50,910 --> 00:45:53,950
+الستبتوكوكاية إلها كابسول و هي عبارة عن anti
+
+611
+00:45:53,950 --> 00:45:56,870
+-phagocytic غالبا ال strings اللي إلها كابسول
+
+612
+00:45:56,870 --> 00:46:01,670
+بتكون ميوكاين، من أشهر الستبتوكوكاية اللي إلها ..
+
+613
+00:46:01,670 --> 00:46:03,470
+من أشهر الستبتوكوكاية اللي إلها كابسول
+
+614
+00:46:10,920 --> 00:46:14,880
+الـ streptococci مجموعة ضخمة، ضخمة ضخمة كبيرة في
+
+615
+00:46:14,880 --> 00:46:18,860
+منها بناكلوها احنا مع اللبن، يعني بيستخدموها ل ..
+
+616
+00:46:18,860 --> 00:46:23,500
+اسمها streptococcus lactis streptococcus lactis
+
+617
+00:46:23,500 --> 00:46:27,280
+إنها بتعمل ال .. ال .. ال lactic acid من الحليب
+
+618
+00:46:27,280 --> 00:46:33,480
+ففي منها pathogenic وفي منها non pathogenic فتم
+
+619
+00:46:33,480 --> 00:46:37,540
+تصنيفها بطريقتين، طريقة ال homolysis فاكرينها؟
+
+620
+00:46:37,540 --> 00:46:41,710
+Alpha, Beta, Gammaهو الطريقة التانية هال يلا
+
+621
+00:46:41,710 --> 00:46:45,090
+انبسطوا الآن العالمة اللي موجودة بكورة اسمها علمة
+
+622
+00:46:45,090 --> 00:46:49,850
+اسمها Rebecca Lansfield Rebecca Lansfield أنضت
+
+623
+00:46:49,850 --> 00:46:53,930
+حياتها في تصنيف لستريبتو كوكاي based on a C
+
+624
+00:46:53,930 --> 00:46:56,530
+-Polysaccharide موجود في ال cell wall تبع لستريبتو
+
+625
+00:46:56,530 --> 00:46:59,790
+كوكا يعني صنفت البكتيريا based on antigenic
+
+626
+00:46:59,790 --> 00:47:02,990
+structure C-Polysaccharide موجود في من ال cell
+
+627
+00:47:02,990 --> 00:47:08,870
+wall وهو بقى عن surface antigen الان
+
+628
+00:47:09,410 --> 00:47:12,530
+لما جينا نجمع كل ال microbiota اللى اسمها stirt
+
+629
+00:47:12,530 --> 00:47:17,150
+coccus اللى جينا انه فيه بينطبق عليها ال system
+
+630
+00:47:17,150 --> 00:47:20,990
+تبع Rebecca Lansfield وفيه بينطبقش عليها فاش صموها
+
+631
+00:47:20,990 --> 00:47:25,990
+groupable و non groupable groupable يعني .. يعني
+
+632
+00:47:25,990 --> 00:47:30,430
+يمكن تصنيفها بتصنيف Rebecca Lansfield و التانية
+
+633
+00:47:30,430 --> 00:47:34,550
+non groupable معناها تحكي انه لا تمتلكلاتمتلك الـ
+
+634
+00:47:34,550 --> 00:47:38,510
+Antigenات اللي بلد عليها Rebecca Lansfield اللي هو
+
+635
+00:47:38,510 --> 00:47:43,710
+تصنيفها حصل منها Non-Growable من أهم المجموعات A,
+
+636
+00:47:43,870 --> 00:47:47,930
+B و D الـ C و الـ G و ال F أقل الأهمية لكن اللي هم
+
+637
+00:47:47,930 --> 00:47:51,330
+الـ A و ال B و ال D هم اللي هندرسهم خلال المحاضرة
+
+638
+00:47:51,330 --> 00:47:54,210
+اللي جاية و هي بس بعد السؤال الأخرى في محاضرتنا
+
+639
+00:47:54,210 --> 00:47:57,450
+الـ Non-Growable تشمل واحد من الميكروبات المهم جدا
+
+640
+00:47:57,450 --> 00:48:01,850
+اللي هي Steptococcus pneumoniaو إلها شبيه مورود في
+
+641
+00:48:01,850 --> 00:48:06,050
+الفيلم بشكل كبير اسمه Stratococcus Mutans و بتسبب
+
+642
+00:48:06,050 --> 00:48:10,710
+Dental Chaos او تسوص اللي هو الاسم باكن كل حاجة
+
+643
+00:48:10,710 --> 00:48:12,970
+انهينا محاضرتنا و ان شاء الله نكمل مرة القاهرة
+
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/vbNOwwvDE6k_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/vbNOwwvDE6k_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..bb30d5069b8557a1ee7601106af3f16531da6803
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/vbNOwwvDE6k_raw.json
@@ -0,0 +1 @@
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نوع من ال competition بينها وبين ال host لأن ال host بده ال Iron وانا بده ال Iron مين بياخده Iron أكتر هو اللي بيكون مسيطر على ال .. ال .. الوضع في ال .. في ال infection", "tokens": [3555, 2655, 29399, 7435, 14407, 2423, 39184, 22130, 43230, 46599, 2655, 29399, 7435, 14407, 2423, 22130, 46326, 4032, 27842, 48812, 27842, 2423, 22130, 43230, 39894, 29399, 7435, 14407, 13672, 1829, 39896, 2423, 5996, 76, 546, 3505, 5296, 15042, 8717, 9381, 49628, 9154, 31767, 5172, 9307, 9673, 16095, 3660, 440, 3485, 295, 264, 5996, 76, 546, 3505, 281, 7983, 13720, 43242, 9673, 28543, 2288, 15844, 16712, 4587, 41193, 2423, 13720, 37037, 15730, 8717, 39648, 18871, 11296, 9154, 2423, 7566, 425, 655, 479, 15104, 13672, 1829, 8978, 11296, 8717, 45367, 9154, 2423, 6211, 49374, 11296, 46599, 9957, 2423, 3975, 5296, 33456, 2423, 3975, 47525, 3224, 2423, 13720, 4032, 7649, 995, 47525, 3224, 2423, 13720, 3714, 9957, 4724, 1829, 47283, 3215, 3224, 13720, 5551, 4117, 2655, 2288, 31439, 13672, 1829, 4724, 1829, 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"temperature": 1.0}, {"id": 51, "seek": 122541, "start": 1203.05, "end": 1225.41, "text": "الشكل التالت من ال salmonellosis اللى هو ال septicemia و اللى قولنا اكتر مايكروب بيعزل منه اللى هو salmonella cholera suis لكن it could be caused by any type of salmonella يعني كتير من ال salmonella ممكن تسبب septicemia لكن ال cholera suis هي أشهر عزلة نازلها من ال septicemia", "tokens": [6027, 8592, 28820, 16712, 6027, 2655, 9154, 2423, 1845, 76, 546, 285, 8211, 13672, 7578, 31439, 2423, 23891, 299, 14058, 4032, 13672, 7578, 12174, 12610, 8315, 1975, 4117, 2655, 2288, 19446, 1829, 4117, 32887, 3555, 4724, 1829, 3615, 11622, 1211, 9154, 3224, 13672, 7578, 31439, 1845, 76, 546, 3505, 20961, 1663, 7624, 44381, 309, 727, 312, 7008, 538, 604, 2010, 295, 1845, 76, 546, 3505, 37495, 22653, 9122, 2655, 13546, 9154, 2423, 1845, 76, 546, 3505, 3714, 43020, 6055, 35457, 3555, 23891, 299, 14058, 44381, 2423, 20961, 1663, 7624, 39896, 5551, 8592, 3224, 2288, 6225, 11622, 37977, 8717, 31377, 1211, 11296, 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"text": "في المنطقة هذه القنبوبة هذه القنبوبة اللي هي ال control يعني البكتيريا اللي مش مزرعة اللي مش مزرعة عبارة عن أيش مكوّنة من أيش من slant و butt يارتن هحط على لوحة slant و butt الآن بتزرع البكتيريا عن طريق حقنها في ال butt و من ثم زيجزاج على ال slant كويس؟", "tokens": [41185, 9673, 1863, 9566, 28671, 29538, 25062, 1863, 3555, 37746, 3660, 29538, 25062, 1863, 3555, 37746, 3660, 13672, 1829, 39896, 2423, 1969, 37495, 22653, 29739, 4117, 2655, 13546, 25528, 13672, 1829, 37893, 3714, 11622, 2288, 27884, 13672, 1829, 37893, 3714, 11622, 2288, 27884, 6225, 3555, 9640, 3660, 18871, 36632, 8592, 3714, 4117, 2407, 11703, 1863, 3660, 9154, 36632, 8592, 9154, 1061, 394, 4032, 6660, 7251, 9640, 2655, 1863, 8032, 5016, 9566, 15844, 45164, 5016, 3660, 1061, 394, 4032, 6660, 6024, 48506, 39894, 11622, 2288, 3615, 29739, 4117, 2655, 13546, 25528, 18871, 23032, 16572, 4587, 11331, 4587, 1863, 11296, 8978, 2423, 6660, 4032, 9154, 38637, 2304, 30767, 1829, 7435, 11622, 26108, 15844, 2423, 1061, 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\ No newline at end of file
diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/vu8Y--lCMY4.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/vu8Y--lCMY4.srt
new file mode 100644
index 0000000000000000000000000000000000000000..94bc54c3814e9d565156f27c15d44c3f7564a9c4
--- /dev/null
+++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/vu8Y--lCMY4.srt
@@ -0,0 +1,1171 @@
+1
+00:00:20,570 --> 00:00:23,290
+بسم الله الرحمن الرحيم نكمل إن شاء الله من حيث ما 
+
+2
+00:00:23,290 --> 00:00:26,630
+انتهينا بمراجعة سريعة على ال physical agents وكان 
+
+3
+00:00:26,630 --> 00:00:31,110
+أول physical agent هو ال heat واتفقنا على أن ال heat 
+
+4
+00:00:31,110 --> 00:00:34,530
+ممكن تقسم إلى قسمين القسم الأول كان بيتكلم معانا 
+
+5
+00:00:34,530 --> 00:00:37,470
+wet heat وأخذنا مثل على ال wet heat اللي هي ال
+
+6
+00:00:37,470 --> 00:00:40,270
+boiling وال boiling زي ال sodium bicarbonate أو 
+
+7
+00:00:40,270 --> 00:00:44,890
+بيكربونات ال sodium اللي هي العامية ال carbon اللي 
+
+8
+00:00:44,890 --> 00:00:49,140
+معناها carbon  ونحن والصنف الثاني كان هو ال
+
+9
+00:00:49,140 --> 00:00:51,340
+pasteurization وشوفنا أشكال مختلفة من ال
+
+10
+00:00:51,340 --> 00:00:56,840
+pasteurization وشوفنا أنه زيادة عشر درجات مئوية من 
+
+11
+00:00:56,840 --> 00:01:01,680
+62 إلى 72 قدرت الزمن من 30 دقيقة إلى 15 ثانية 
+
+12
+00:01:02,670 --> 00:01:07,110
+واتكلمنا أيضًا عن نوع آخر منه سميناه ultra-high
+
+13
+00:01:07,110 --> 00:01:11,830
+treatment pasteurization لكن لا واحدة من الأولى لا
+
+14
+00:01:11,830 --> 00:01:13,650
+الأولى ولا الثانية كانوا بيؤدوا إلى ال
+
+15
+00:01:13,650 --> 00:01:16,910
+sterilization لكن الثالثة اللي هي كانت steam under 
+
+16
+00:01:16,910 --> 00:01:21,490
+pressure كانت اللي هي ال autoclave أدى إلى اللي هو 
+
+17
+00:01:21,490 --> 00:01:25,930
+التعقيم وقلنا at 121 Degrees Integrate for 15
+
+18
+00:01:25,930 --> 00:01:29,390
+minutes to 20 minutes at 15 psi لو كنا pound per
+
+19
+00:01:29,390 --> 00:01:35,230
+square inch صنف ثانية كانت من الحلقة الأولى اللي 
+
+20
+00:01:35,230 --> 00:01:37,690
+شكله اللي قدامكم اللي هو  جهاز ال Autoclave بشكل
+
+21
+00:01:37,690 --> 00:01:42,880
+مبسط عبارة عن heater إذا شايفين تحت هنا هذا ال
+
+22
+00:01:42,880 --> 00:01:48,800
+heater مغمور بالماء وفوق الماء في حاجز هذا حاجز
+
+23
+00:01:48,800 --> 00:01:53,180
+مثقب يعني يسمح لبخار الماء بالخروج ونضع عليه
+
+24
+00:01:53,180 --> 00:01:56,020
+الأشياء اللي بنعقمها يعني لو بنعقم شيء معين مثلًا
+
+25
+00:01:56,020 --> 00:02:00,920
+media أو glassware بنحطهم على هذا السطح ونغلق
+
+26
+00:02:00,920 --> 00:02:06,100
+بإغلاق محكم يعني لأننا نتكلم هنا عن بخار ماء
+
+27
+00:02:06,100 --> 00:02:10,640
+وبالتالي بخار الماء لازم يظل في ال container
+
+28
+00:02:10,640 --> 00:02:13,720
+نفسه ولا يتسرب له تسريب عشان يعطي الحرارة اللي
+
+29
+00:02:13,720 --> 00:02:17,360
+نحن بدنا إياه ليصل إلى 121 درجة مئوية لكي نشكل ضغط
+
+30
+00:02:17,360 --> 00:02:20,660
+بالأحرار ويرتفع لنا الحرارة الشكل اللي أمامكم 
+
+31
+00:02:20,660 --> 00:02:25,120
+هذا هو الشكل الحقيقي laptop view of an autoclave،
+
+32
+00:02:25,120 --> 00:02:28,680
+هذا هتشوفوه في المختبر إن شاء الله عندنا لاحظوا
+
+33
+00:02:28,680 --> 00:02:36,060
+الغطاء اللي فوق هنا يعني بدنا نبذل قوة كبيرة عشان
+
+34
+00:02:36,060 --> 00:02:43,080
+نغلق الجهاز إغلاق محكم الشراط اللي بقى بنا هنا هي 
+
+35
+00:02:43,080 --> 00:02:47,220
+أزرار للتشغيل وساعات من خلالها بنعرف الضغط وبنعرف 
+
+36
+00:02:47,220 --> 00:02:53,660
+ال time وبنعرف ال temperature طبعًا ال modern
+
+37
+00:02:53,660 --> 00:02:58,870
+autoclave تطورت شوية صار فيها إمكانية أنه .. يعني 
+
+38
+00:02:58,870 --> 00:03:01,410
+automated cycles يعني إيش .. إيش .. إيش يعني
+
+39
+00:03:01,410 --> 00:03:04,910
+automated cycles؟ نحن بنبرمجها .. بقى .. بنقول له
+
+40
+00:03:04,910 --> 00:03:07,690
+cycle one أو cycle two حسب البرنامج اللي موجودة و
+
+41
+00:03:07,690 --> 00:03:12,930
+خلاص نترك الجهاز لوحده بيشتغل هو بيرفع
+
+42
+00:03:12,930 --> 00:03:16,690
+درجة الحرارة 121 درجة مئوية و يبدأ بعد ذلك يعد
+
+43
+00:03:16,690 --> 00:03:21,350
+لما تصل الحرارة 121 يبدأ يعد الزمن اللي أنا 
+
+44
+00:03:21,350 --> 00:03:24,690
+مخصصه له أو مبرمجة له في ال cycle خمس عشر أو عشرين
+
+45
+00:03:24,690 --> 00:03:30,300
+دقيقة لو درجة الحرارة نزلت عن طبعًا بيفصل على 121
+
+46
+00:03:30,300 --> 00:03:35,780
+الحرارة بيفصل لو نزلت عن 121 بيرجع بيشتغل مرة ثانية لو
+
+47
+00:03:35,780 --> 00:03:40,420
+ارتفعت عن 121 بيفصل وبالتالي بيظل محافظ على درجة
+
+48
+00:03:40,420 --> 00:03:45,440
+محددة للزمن اللي نحن حددناه في كمال modification أنه
+
+49
+00:03:45,440 --> 00:03:48,160
+.. طب نحن بدنا نكون ولا wet wet يعني الأشياء
+
+50
+00:03:48,160 --> 00:03:53,640
+اللي بتطلع من الرتبة في autoclaves زودت من ما يسمى 
+
+51
+00:04:09,040 --> 00:04:12,260
+الصنف الثاني كان من ال من ال physical agent
+
+52
+00:04:12,260 --> 00:04:15,300
+اللي هو radiation ونقسمه لقسمين أيضًا القسم 
+
+53
+00:04:15,300 --> 00:04:18,860
+الأول بيتكلم عن ionizing radiation والثاني عن non
+
+54
+00:04:18,860 --> 00:04:23,000
+-ionizing radiationالأولى بيمثلها اللي هو ال ال
+
+55
+00:04:23,000 --> 00:04:27,260
+ال X-ray والثاني بيمثلها ال ultraviolet
+
+56
+00:04:27,260 --> 00:04:32,380
+light  نأخذ ال ultraviolet light ال UV يعني من ال
+
+57
+00:04:32,380 --> 00:04:36,640
+ال الإشعاعات اللي أنتم بتعرفوا أنها
+
+58
+00:04:36,640 --> 00:04:40,800
+مؤذية للإنسان وبالذات مؤذية للعين ده ال corner of
+
+59
+00:04:40,800 --> 00:04:45,400
+the eye ومؤذية للجلد وبتسمعوا أنه ثقب ال ozone
+
+60
+00:04:47,320 --> 00:04:50,540
+سبب بأنه كمية كبيرة من ال UV اللي جاية من الشمس
+
+61
+00:04:50,540 --> 00:04:53,880
+تدخل الأرض يعني كان هو يحدث ال UV هذه فنسبة ال
+
+62
+00:04:53,880 --> 00:04:57,280
+skin cancer زادت يعني معناه الحكي أنه أنا ما أجي أستخدم 
+
+63
+00:04:57,280 --> 00:05:01,340
+ال UV بيننا يعني زي ال neon أو الإضاءة
+
+64
+00:05:01,340 --> 00:05:04,340
+اللي موجودة هنا ونحن بنشتغل في المستشفى أو في 
+
+65
+00:05:04,340 --> 00:05:07,600
+المطبخ تكون شغالة ونحن بنشتغل معها لا لأنها
+
+66
+00:05:07,600 --> 00:05:10,480
+مؤذية للعين وبنا مؤذية للجلد وممكن تعمل بنا 
+
+67
+00:05:10,480 --> 00:05:18,220
+cancerشوفوا ال wavelength المحدد 250 إلى 260 نانومتر
+
+68
+00:05:18,220 --> 00:05:21,860
+هذا ال wavelength الأفضل اللي بيكون فيه قتل على ما 
+
+69
+00:05:21,860 --> 00:05:24,540
+يكون عند هذا ال wavelength والسبب بتكونوا تعرفوا
+
+70
+00:05:24,540 --> 00:05:30,420
+ليش السبب السبب أنه هذا ال wavelength هو الأفضل لل
+
+71
+00:05:30,420 --> 00:05:33,080
+absorbance لل purines وال pyrimidines بتعرفوا ال
+
+72
+00:05:33,080 --> 00:05:35,060
+purines وال pyrimidines هم اللي بشكل القواعد 
+
+73
+00:05:35,060 --> 00:05:40,610
+بتاعت مين؟ ال DNA طب ال UV إيش بتاعتها مع ال UV؟ ال UV
+
+74
+00:05:40,610 --> 00:05:45,650
+بتشوف اثنين ثايمين جنب بعض ثايمين اللي هو ال T،
+
+75
+00:05:45,650 --> 00:05:49,050
+بتروح تعمل cross link بينهم ال UV بتعمل cross
+
+76
+00:05:49,050 --> 00:05:55,170
+link بينهم فالآن هم مش ثايمين هم ثايمين دائمًا ال
+
+77
+00:05:55,170 --> 00:05:58,710
+DNA polymerase أو ال RNA polymerase ما بيقف يقرأ 
+
+78
+00:05:58,710 --> 00:06:03,050
+إلا الأحرف الأربعة تبعونه أدنين جوانين سايتوسين
+
+79
+00:06:03,050 --> 00:06:06,630
+وثايمين الآن لما بيشوف الاثنين مع بعض مربوطين ببعض 
+
+80
+00:06:06,630 --> 00:06:11,390
+أنه ثايمين أو أن أصلهم ثايمين طب يقفز عنهم طب إذا 
+
+81
+00:06:11,390 --> 00:06:14,450
+قفز عنهم مش ستصبح لنا deletion طب إذا صار لنا 
+
+82
+00:06:14,450 --> 00:06:18,450
+deletion مش ستصبح لنا frame shift mutation طيب 
+
+83
+00:06:18,450 --> 00:06:22,490
+تخيلوا الآن كل ما زاد زمن التعرض ال explore
+
+84
+00:06:22,490 --> 00:06:27,980
+time كلما زاد فرصة من الصوفيا الناه ال mutation طب
+
+85
+00:06:27,980 --> 00:06:30,680
+إذا صار ال mutation في أكثر من مكان وصار ال
+
+86
+00:06:30,680 --> 00:06:33,980
+mutation في essential genes يعني جينات أساسية أو
+
+87
+00:06:33,980 --> 00:06:37,480
+رئيسية لحياة الخلية يبقى إيش يصير في الخلية الآن؟ 
+
+88
+00:06:37,480 --> 00:06:43,300
+بتموت هيك عرفنا كيف ال UV بتقتل إذا كلما زاد زمن 
+
+89
+00:06:43,300 --> 00:06:49,520
+التعرض كلما زاد فرصة الموت اللي هو البكلي طبعًا معروف
+
+90
+00:06:49,520 --> 00:06:53,500
+أن قدرة تأثيره على قتل ال vegetative cells أعلى
+
+91
+00:06:53,500 --> 00:06:59,320
+بكثير من قدرة قتله على ال spores وقد نحتاج يا 
+
+92
+00:06:59,320 --> 00:07:04,000
+بنات عشرة أضعاف الجرعة اللي نحتاجها لمين؟ لل
+
+93
+00:07:04,000 --> 00:07:06,960
+vegetative cell يعني ال spores are more resistant
+
+94
+00:07:06,960 --> 00:07:10,560
+than vegetative cells for UV كده
+
+95
+00:07:14,830 --> 00:07:19,230
+في كمان ميكانيزم لكن أقل أهمية لل UV أنه ممكن تضيف
+
+96
+00:07:19,230 --> 00:07:22,570
+هيدروكسيل جروب لقواعد نيتروجينية وبالتالي بتعمل
+
+97
+00:07:22,570 --> 00:07:28,300
+أيضًا destruction لمين؟ لل DNA ال ال ال ال
+
+98
+00:07:28,300 --> 00:07:29,180
+ال ال ال ال 
+
+99
+00:07:29,180 --> 00:07:29,220
+ال ال ال ال 
+
+100
+00:07:29,220 --> 00:07:29,700
+ال ال ال ال 
+
+101
+00:07:29,700 --> 00:07:29,800
+ال ال ال ال 
+
+102
+00:07:29,800 --> 00:07:30,260
+ال ال ال ال 
+
+103
+00:07:30,260 --> 00:07:35,580
+ال ال ال ال 
+
+104
+00:07:35,580 --> 00:07:37,620
+ال ال ال ال 
+
+105
+00:07:37,620 --> 00:07:38,260
+ال ال ال ال 
+
+106
+00:07:38,260 --> 00:07:39,760
+ال ال ال ال 
+
+107
+00:07:39,760 --> 00:07:41,860
+ال ال ال ال 
+
+108
+00:07:41,860 --> 00:07:48,220
+ال ال ال ال 
+
+109
+00:07:48,220 --> 00:07:54,180
+ال 
+
+110
+00:07:54,220 --> 00:07:58,380
+بيكونوا تعرفوا أن ال repair system هذا فيه منه
+
+111
+00:07:58,380 --> 00:08:02,820
+error free إيش يعني error free؟ يعني زي ال
+
+112
+00:08:02,820 --> 00:08:07,720
+mechanic الشاطر بيرجع الخراب كما هو يعني بيصلحه
+
+113
+00:08:07,720 --> 00:08:13,940
+ده صحيح خالي من الخطأ وفي نوعية أخرى اسمها error
+
+114
+00:08:13,940 --> 00:08:21,300
+prone يعني قد يصلح الخطأ بخطأ آخر يعني مش دائمًا
+
+115
+00:08:21,300 --> 00:08:25,440
+التصحيح إيه بيكون دقيق وإلا ما كان وإلا ما كان
+
+116
+00:08:25,440 --> 00:08:28,000
+مصر عندنا ميوتين بالمرة مدهن مدهن فيه repair
+
+117
+00:08:28,000 --> 00:08:32,440
+mechanism وفي repair mechanism بتتم من خلال الضوء
+
+118
+00:08:32,440 --> 00:08:36,560
+بيسموها photo repair وفي mechanism لل repair بتتم
+
+119
+00:08:36,560 --> 00:08:40,400
+في الظلام وبيسميها dark repair يعني في photo
+
+120
+00:08:40,400 --> 00:08:44,320
+reactivation وفي عندنا اللي هو ال dark repair لكن
+
+121
+00:08:44,320 --> 00:08:51,500
+بتدريفهم شغل أن ال UV بيواقعها وإشكالياتها
+
+122
+00:08:51,500 --> 00:08:59,380
+المتعلقة بال بال بال skin damage وبال eye
+
+123
+00:08:59,380 --> 00:09:04,660
+damage في الحالتين هذا منع استخدام منع استخدام
+
+124
+00:09:04,660 --> 00:09:09,540
+ال UV بكثرة في الطب تعالوا نأخذ بعض الأمثلة على
+
+125
+00:09:09,540 --> 00:09:14,340
+استخدامات ال UV في الطب واحد operating room
+
+126
+00:09:14,340 --> 00:09:20,740
+disinfection أو sterilization عن طريق وضع نيونات
+
+127
+00:09:20,740 --> 00:09:27,720
+لكن هل بنقدر نشتغل و النيونات هي مضارة؟ متى بنشتغل؟
+
+128
+00:09:27,720 --> 00:09:33,380
+بعد الانتهاء بمعنى آخر قبل البدء بعمليات جديدة
+
+129
+00:09:33,380 --> 00:09:37,860
+ممكن نعقم الغرفة عن طريق الـ UV light في كمان
+
+130
+00:09:37,860 --> 00:09:42,060
+استخدام مش مذكور على اللوحة اللي هو في الـ water
+
+131
+00:09:42,060 --> 00:09:47,440
+disinfection تخيلوا معايا الوضع التالي مجرى مائي
+
+132
+00:09:47,440 --> 00:09:52,480
+سمك الماء فيه يعني عمق الماء بمعنى آخر فيه رقيق
+
+133
+00:09:52,480 --> 00:10:00,100
+جدا و بيمر عبر مجرى مائي طويل كله مغطى بـ UV Lights
+
+134
+00:10:00,100 --> 00:10:05,660
+فخلينا نقول إن الماء بتعرض لمسافة طويلة كلمة مسافة
+
+135
+00:10:05,660 --> 00:10:08,480
+ما عندنا حكي عنه إن الماء اللي هنا لما يقطع
+
+136
+00:10:08,480 --> 00:10:14,310
+المسافة بده إيه؟ بده زمن ففيه زمن تعرض معروف، ممكن
+
+137
+00:10:14,310 --> 00:10:18,610
+برضه نستخدم الـ UV for water disinfection، water 
+
+138
+00:10:18,610 --> 00:10:21,310
+disinfection، في المعامل بتعرفوا اللي أنا في الـ
+
+139
+00:10:21,310 --> 00:10:23,950
+laminar hood، هذه الـ ... الـ ... الـ ... الـ safety cabinet،
+
+140
+00:10:23,950 --> 00:10:28,470
+بيكون فيها برضه UV، بعد ما بنخلص شغلنا، بننزل الـ
+
+141
+00:10:28,470 --> 00:10:32,010
+... الـ plastic shield، بنشغل الـ UV، حتى نعقم الـ
+
+142
+00:10:32,010 --> 00:10:35,720
+area اللي كنا نشتغل فيها، إذا الـ UV له استخدامات
+
+143
+00:10:35,720 --> 00:10:40,360
+جيدة في الطب لكن كمان مرة كان ممكن ممتاز لو احنا
+
+144
+00:10:40,360 --> 00:10:44,580
+قدرنا نشتغل بالـ UV موجود بس للأسف لإنه بدورنا
+
+145
+00:10:44,580 --> 00:10:50,200
+ما قدرنا اليوم، في على فكرة تقنية شبيهة، طلعوا blue
+
+146
+00:10:50,200 --> 00:10:56,060
+light عنده مقدرة على قتل البكتيريا و لا يؤذي
+
+147
+00:10:56,060 --> 00:11:00,480
+الإنسان، فاعتقد أن هذه التقنية هتحل مشاكل كبيرة في
+
+148
+00:11:00,480 --> 00:11:03,720
+الـ infection control في المستشفى، لسه ما شاعت
+
+149
+00:11:08,870 --> 00:11:12,630
+طيب الـ X-ray احنا قولنا الـ X-ray عبارة عن high
+
+150
+00:11:12,630 --> 00:11:16,730
+energy radiation
+
+151
+00:11:16,730 --> 00:11:22,870
+that can have penetration power أكثر من اللي هو الـ
+
+152
+00:11:22,870 --> 00:11:26,430
+UV و بتقتل البكتيريا عن طريق إنتاج لـ free
+
+153
+00:11:26,430 --> 00:11:30,510
+radicals ليش عن الـ free radicals؟ للشقوق الحرة الـ
+
+154
+00:11:30,510 --> 00:11:36,360
+H و الـ OH، الآن الـ H و الـ OH لما بيرتبطوا مع جزئية
+
+155
+00:11:36,360 --> 00:11:40,740
+الماء أو OH بيرتبط مع OH، بايش بيطلعوا؟ H2O2 يعني
+
+156
+00:11:40,740 --> 00:11:44,240
+hydrogen peroxide و كل مرة بدنا نعيد نفس الاسطوانة
+
+157
+00:11:44,240 --> 00:11:48,920
+و نقول لكم أن الـ H2O2 هو a very powerful oxidizing
+
+158
+00:11:48,920 --> 00:11:52,200
+agent و أخدناه من ضمن الـ disinfectants من ضمن الـ
+
+159
+00:11:52,200 --> 00:11:54,760
+chemical disinfectants اللي بيأثروا على الـ protein
+
+160
+00:11:54,760 --> 00:12:01,540
+و على الـ DNA، الآن كمان مرة الـ spores
+
+161
+00:12:06,550 --> 00:12:11,510
+مثل اثران من الـ vegetative cells بالـ X-ray قد يعود
+
+162
+00:12:11,510 --> 00:12:15,690
+السبب في ذلك إلى عدم احتواء الـ spores الماء، طبعا
+
+163
+00:12:15,690 --> 00:12:20,190
+ما هي علاقة الماء في الموضوع؟ الماء من الـ ... ومش هو
+
+164
+00:12:20,190 --> 00:12:25,690
+المنتج لمن؟ الشقوق الحرة قلنا؟ الـ H2 الـ OH والـ H
+
+165
+00:12:25,690 --> 00:12:29,830
+جايين من الماء فبالتالي ما فيش ماء يجيب وما فيش شقوق
+
+166
+00:12:29,830 --> 00:12:34,280
+حرة كافية لقتل اللي هو الـ spores، في معلومة كثير
+
+167
+00:12:34,280 --> 00:12:37,340
+كويسة بتقول أن الـ sulfhydryl containing compounds
+
+168
+00:12:37,340 --> 00:12:40,000
+such as amino acids cysteine can protect DNA from
+
+169
+00:12:40,000 --> 00:12:46,520
+free radical attacks، وجود بروتينات تحتوي على
+
+170
+00:12:46,520 --> 00:12:51,760
+cysteine أو أو amino acids تحتوي على sulfhydryl
+
+171
+00:12:51,760 --> 00:12:58,360
+group بيساعد الـ DNA في الحماية من مين؟ من الـ X-ray
+
+172
+00:12:58,360 --> 00:13:02,920
+هذا الظهر هو اللي شافوه شافوه فيه أنه فيه بكتيريا
+
+173
+00:13:02,920 --> 00:13:06,760
+اسمها radio ... radio ... radio bacteria، هذه الظاهرة
+
+174
+00:13:06,760 --> 00:13:12,560
+... هذه الظاهرة لما درسوا الـ ... الـ ... التركيب تبع
+
+175
+00:13:12,560 --> 00:13:16,780
+الخلية وردوا أنه بالفعل فيها plenty of self ...
+
+176
+00:13:16,780 --> 00:13:19,960
+self-hydrating containing اللي هو amino acids
+
+177
+00:13:22,980 --> 00:13:29,200
+في آلية أخرى للـ killing بالـ X-Ray وهو تكسير الـ
+
+178
+00:13:29,200 --> 00:13:31,700
+covalent bonds في الـ DNA نفسه يعني الـ DNA نفسه
+
+179
+00:13:31,700 --> 00:13:36,920
+بيتكسر من الـ high energy اللي طالعة من الـ X-Ray لكن
+
+180
+00:13:36,920 --> 00:13:41,520
+بقول إن الآلية دي أقل أهمية أيضا من الآلية رقم 1
+
+181
+00:13:41,520 --> 00:13:47,180
+اللي هي عبر الـ free radicals، الـ X-Ray ممكن تستخدم
+
+182
+00:13:47,180 --> 00:13:52,150
+في الطب في تعقيم الـ heat sensitive items، Heat
+
+183
+00:13:52,150 --> 00:13:55,770
+sensitive items يعني مركبات أو مكونات تتأثر بمين؟
+
+184
+00:13:55,770 --> 00:13:58,970
+بالحرارة، كلمة تتأثر بالحرارة يعني بصير لها damage
+
+185
+00:13:58,970 --> 00:14:02,330
+بالحرارة، such as sutures بتوع الخيوط اللي
+
+186
+00:14:02,330 --> 00:14:06,970
+بيستخدموها في غرز الجراحية and surgical gloves
+
+187
+00:14:06,970 --> 00:14:11,330
+اللي هي الجوانتي الجراحية المعقمة أيضا and
+
+188
+00:14:11,330 --> 00:14:14,570
+plastic items such as syringes، بس بدكم تتذكروا
+
+189
+00:14:14,570 --> 00:14:22,060
+شغلة أنه clean first، التعقيم زمان كان مش مشكلة أنه
+
+190
+00:14:22,060 --> 00:14:26,000
+يكون الأشياء مش نظيفة، ما احنا بنعقم، لكن لما
+
+191
+00:14:26,000 --> 00:14:29,700
+اكتشفنا أن الـ remains ... الـ remains يعني بقايا
+
+192
+00:14:29,700 --> 00:14:34,360
+البكتيريا الميتة أو الفطريات الميتة قد تسبب مرض
+
+193
+00:14:34,360 --> 00:14:37,660
+للإنسان زي الـ endotoxin زي الـ libotic acid زي
+
+194
+00:14:37,660 --> 00:14:41,180
+الـ peptidoglycan بعض الـ toxins الأخرى اللي سميناها
+
+195
+00:14:41,180 --> 00:14:47,430
+pyrogens فبالتالي كنا clean first، النوع الثالث أو صنف
+
+196
+00:14:47,430 --> 00:14:49,730
+الثالث من الـ ... من الـ physical agent اللي هو الـ
+
+197
+00:14:49,730 --> 00:14:53,250
+filtration و الـ filtration is a mechanical removal
+
+198
+00:14:53,250 --> 00:14:59,390
+of microorganism by passing through a membrane
+
+199
+00:14:59,390 --> 00:15:06,290
+filter، membrane filter يعني منقي أو مرشح مكون من
+
+200
+00:15:06,290 --> 00:15:13,290
+غلاف أو من غشاء بسيط، رقيق، المسامات تبعته ما
+
+201
+00:15:13,290 --> 00:15:17,170
+بتفوت البكتيريا أو تشتغل بأي طريقة ثانية، بتاع الكهربية
+
+202
+00:15:17,170 --> 00:15:21,150
+الساكنة ... الكهربية الساكنة قد تجعل الأشياء
+
+203
+00:15:21,150 --> 00:15:27,130
+الصغيرة تلتصق بـ ... ببعضها البعض، فممكن يكون فيروسات
+
+204
+00:15:27,130 --> 00:15:31,110
+حجمها أصغر من الـ ... من الـ pore size تبع الـ ... الـ
+
+205
+00:15:31,110 --> 00:15:34,510
+membrane لكن ما تمرش عبره لإنه التثقت عبر
+
+206
+00:15:34,510 --> 00:15:38,390
+الكهربية الساكنة بالـ membrane، الـ pore size صغير
+
+207
+00:15:38,390 --> 00:15:42,030
+بيحدث، و في نفس الوقت في خاصية ما قلنا كمان مرة يا شباب
+
+208
+00:15:42,030 --> 00:15:47,100
+اللي هي الـ electrostatic attraction، من أشهر الـ ...
+
+209
+00:15:47,100 --> 00:15:49,460
+الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... 
+
+210
+00:15:49,460 --> 00:15:49,880
+... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ...
+
+211
+00:15:49,880 --> 00:15:49,920
+الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ...
+
+212
+00:15:49,920 --> 00:15:50,300
+... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ...
+
+213
+00:15:50,300 --> 00:15:52,520
+الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ...
+
+214
+00:15:52,520 --> 00:15:57,640
+... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ...
+
+215
+00:15:57,640 --> 00:15:58,740
+الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ...
+
+216
+00:15:58,740 --> 00:16:01,700
+... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ...
+
+217
+00:16:01,700 --> 00:16:11,000
+الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... 
+
+218
+00:16:15,040 --> 00:16:40,700
+خلينا نحاول نرسم جهاز صغير، تخيلوا
+
+219
+00:16:40,700 --> 00:16:42,700
+معي هنا مفتوح من الطرفين
+
+220
+00:16:45,530 --> 00:16:50,350
+جالس على كأس برضه مفتوح لكنه قاعدة، بنحط عليها الـ
+
+221
+00:16:50,350 --> 00:16:53,850
+filter تمام؟
+
+222
+00:16:53,850 --> 00:16:59,450
+الآن طبعا بنشبكهم مع بعض بطريقة معينة، إذا بمغناطيس
+
+223
+00:16:59,450 --> 00:17:03,130
+أو بمشبك فبنكونوا راكبين فوق بعض وغير منافذين
+
+224
+00:17:03,130 --> 00:17:09,910
+للماء تمام؟ بنصب عليه الماء مثلا 100 مل عبر الـ
+
+225
+00:17:09,910 --> 00:17:14,120
+filter و لأن المسامات صغيرة، بيجدوا ست ساعات،
+
+226
+00:17:14,120 --> 00:17:18,580
+ساعتين، يمكن طول النهار ما ينزلش عشرة ملي، مضطرين
+
+227
+00:17:18,580 --> 00:17:27,540
+احنا نعمل عملية خلخلة الهواء هنا عبر vacuum، شفت
+
+228
+00:17:27,540 --> 00:17:31,820
+خلخلة الهواء عبر عملية اللي هو التفريغ، فالسائل
+
+229
+00:17:31,820 --> 00:17:37,760
+بيضطر ينزل الآن هنا، أي بكتيريا كانت موجودة في
+
+230
+00:17:37,760 --> 00:17:44,460
+المواصل تلاني على الـ membrane filter، بنفكر الغطاء
+
+231
+00:17:44,460 --> 00:17:48,060
+وبناخد الـ membrane بملعقة معقمة وبنحطه على طبق
+
+232
+00:17:48,060 --> 00:17:54,420
+تمام؟ وين فيه بكتيريا هتكون مستعمرة، كل مستعمرة أنا
+
+233
+00:17:54,420 --> 00:17:57,620
+افترضت أنها نشأت من مين؟ من خلية واحدة، وبالتالي
+
+234
+00:17:57,620 --> 00:18:03,600
+أنا بقدر أعد وبقول كان فيه 250 مستعمرة أو وحدة
+
+235
+00:18:03,600 --> 00:18:08,320
+بناء مستعمرة، ايش سميناها هم؟ هم مين؟ Colony Forming Unit
+
+236
+00:18:08,320 --> 00:18:14,330
+وحدة بناء مستعمرة في كل 100 ML من اللي هو العينة هذا
+
+237
+00:18:14,330 --> 00:18:20,770
+فحص معتبر وبيعتمد عليه جزء كبير من مختبرات الصحة
+
+238
+00:18:20,770 --> 00:18:24,250
+العامة، مختبرات الصحة العامة في منها قسم بس بيكون
+
+239
+00:18:24,250 --> 00:18:29,630
+بيرتبطوا لمياه الشرب، بيجي عينات من كل قطاع غزة من
+
+240
+00:18:29,630 --> 00:18:34,430
+كل بلدية قطاع غزة مرتين تقريبا في الشهر وبيفحصها
+
+241
+00:18:34,430 --> 00:18:38,520
+عشان يشوف عدد البكتيريا اللي موجودة فيها، صالح للشرب
+
+242
+00:18:38,520 --> 00:18:41,940
+و لا غير صالح للشرب، طبعا إذا كان فيه بكتيريا من
+
+243
+00:18:41,940 --> 00:18:46,780
+نوع Coliform فهو يكون غير صالح للشرب، الفكرة
+
+244
+00:18:46,780 --> 00:18:49,500
+الثانية اللي بنشتغلها و بنستخدم فيها الـ filtration
+
+245
+00:18:49,500 --> 00:18:54,940
+لما طالباتنا في البحث أو طلابنا في البحث بيشتغلوا
+
+246
+00:18:54,940 --> 00:19:00,340
+مركب و بيدّوا يشوفوا هذا المركب مدى قدرته على قتل
+
+247
+00:19:00,340 --> 00:19:03,160
+الميكروبات، احنا خايفين أن المركب أساسا هم بيشتغلوا
+
+248
+00:19:03,160 --> 00:19:07,670
+فيه بيحضروا فيه يكون ملطّب، نروح بالداخل وعبر
+
+249
+00:19:07,670 --> 00:19:10,410
+membrane filter بس المرة هذه بطريقة مختلفة شوية
+
+250
+00:19:10,410 --> 00:19:19,670
+بتاعة فوق الـ ... السيرنجة، هاد الـ needle وعلى الطرف 
+
+251
+00:19:19,670 --> 00:19:27,850
+التاني هناك بيكون سيرنج ال
+
+252
+00:19:27,850 --> 00:19:32,150
+membrane filter بيكون في plastic container مفتوح
+
+253
+00:19:32,150 --> 00:19:36,440
+للطرفين طرف بيلاسق فيه الneedle وطرف من اللذك فيه
+
+254
+00:19:36,440 --> 00:19:39,320
+اللي هو الـ syringe الآن هي المحلول تبعنا هنا في
+
+255
+00:19:39,320 --> 00:19:41,760
+المنطقة هذه طبعا كلكم بتعرفوا أن الـ syringe
+
+256
+00:19:41,760 --> 00:19:46,040
+بينفك منها والـ needle هي تكون معقّمة لكن ال needle
+
+257
+00:19:46,040 --> 00:19:51,200
+دخلت في ميعاق غير معقّمة بس الـ syringe معقّمة
+
+258
+00:19:51,200 --> 00:19:55,800
+إيه الفاصل بينهم؟ ال membrane filter سحبنا السائل،
+
+259
+00:19:55,800 --> 00:19:59,680
+السائل مر قبل ال filter كل البكتيريا حُجزت فالآن
+
+260
+00:19:59,680 --> 00:20:04,460
+اللي موجود في الـ syringe المرة دي سافر ل .. سافر
+
+261
+00:20:04,460 --> 00:20:08,980
+لإيش؟ للتعقيم لو بدي أعمل urea solution وانتوا
+
+262
+00:20:08,980 --> 00:20:12,780
+قعدتوا .. قعدتم في المختبر درستوا ال .. ال
+
+263
+00:20:12,780 --> 00:20:15,260
+biochemical test و أحد ال biochemical test ال
+
+264
+00:20:15,260 --> 00:20:19,340
+urease ال urease فحص معتمد على إنه الإنزيم بيكسر
+
+265
+00:20:19,340 --> 00:20:24,200
+urea أو بيكسرهاش ال urea لو حطيناها بال autoclave
+
+266
+00:20:24,200 --> 00:20:28,280
+بتتكسر لحالها it's sensitive material فهنضطر لما 
+
+267
+00:20:28,280 --> 00:20:32,140
+نحضركم ال media أن أعقّم لكم ال urea عبر مين
+
+268
+00:20:32,430 --> 00:20:37,370
+الفيلتر يمكن استخدامه كتستنج أو كعملية
+
+269
+00:20:43,790 --> 00:20:49,150
+في ختام المحاضرة بدنا نذكر أهم النقاط اللي ذكرت في
+
+270
+00:20:49,150 --> 00:20:52,230
+المحاضرة كلمة sterilization و disinfection ميزنا
+
+271
+00:20:52,230 --> 00:20:55,650
+بينهم تمييز دقيق absolute والتانية مش absolute
+
+272
+00:20:55,650 --> 00:20:59,930
+التانية بتقولنا إيه؟ بتقولنا اللي هي disinfection
+
+273
+00:20:59,930 --> 00:21:02,850
+بتقولنا إنه reducing the number إيش يعني reducing
+
+274
+00:21:02,850 --> 00:21:07,820
+the number؟ تقليل العدد بحيث أن ال infection is not
+
+275
+00:21:07,820 --> 00:21:10,480
+likely to occur، إيش يعني is not likely to occur؟
+
+276
+00:21:10,480 --> 00:21:13,100
+يعني ما يصير .. في احتمالية حدوث ال infection
+
+277
+00:21:13,100 --> 00:21:16,400
+بتكون قليلة، احتمالية حدوث ال infection بتكون
+
+278
+00:21:16,400 --> 00:21:19,840
+قليلة، هنا في فرق ما بين ال .. ال sterilization و
+
+279
+00:21:19,840 --> 00:21:24,780
+إيه؟ ال disinfection اتكلمنا عن نوعين من ال .. ال
+
+280
+00:21:24,780 --> 00:21:27,560
+.. ال disinfectants، واحد سمينا antiseptic، واحد
+
+281
+00:21:27,560 --> 00:21:31,320
+سمينا disinfectant بحسب اللي هو الاستخدام، وأشهر
+
+282
+00:21:31,320 --> 00:21:35,540
+واحد كان اللي هو ال 70% Ethanol، وعددنين بيشتغل،
+
+283
+00:21:35,540 --> 00:21:39,200
+على ال .. in animate object أو على اللي هو ال ..
+
+284
+00:21:39,200 --> 00:21:44,420
+ال animate object الـ Killing للميكروبات بيكون
+
+285
+00:21:44,420 --> 00:21:48,480
+proportional ل ال concentration اللي هي ال
+
+286
+00:21:48,480 --> 00:21:51,180
+concentration والطعم إيش سميناهم؟ ال concentration
+
+287
+00:21:51,180 --> 00:21:57,380
+والطعم مع بعض سميناهم ال dose اللي هي الجرعة اتفقنا
+
+288
+00:21:57,380 --> 00:22:02,020
+على أنه كمان القتل من قبل ال chemical بينبغي تحت
+
+289
+00:22:02,020 --> 00:22:05,580
+واحد من تلت ميكانزمات الأولى cell membrane limits
+
+290
+00:22:05,580 --> 00:22:10,460
+protein modification DNA modification
+
+291
+00:22:26,960 --> 00:22:30,840
+الوحدة الأخيرة اللي قدامكم هي good summary لكل
+
+292
+00:22:30,840 --> 00:22:33,200
+الأشياء اللي درسناها ونكمل إن شاء الله المرة
+
+293
+00:22:33,200 --> 00:22:33,540
+القادمة