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+{"segments": [{"id": 1, "seek": 3892, "start": 20.92, "end": 38.92, "text": "بسم الله الرحمن الرحيم الحمد لله رب العالمين والصلاة والسلام على أشرف المرسلين سيدنا محمد وعلى آله وصحبه أجمعين السلام عليكم ورحمة الله وبركاته في المحاضرة الماضية تكلمنا عن أهداف طريق تنظيم المجتمع", "tokens": [3555, 38251, 21984, 34892, 5016, 27842, 34892, 5016, 32640, 21542, 2304, 3215, 24976, 3224, 12602, 3555, 18863, 45340, 9957, 16070, 9381, 15040, 3660, 16070, 3794, 37440, 15844, 5551, 8592, 28480, 9673, 2288, 3794, 1211, 9957, 8608, 25708, 8315, 3714, 35571, 3215, 4032, 3615, 23942, 19753, 43761, 4032, 9381, 5016, 3555, 3224, 5551, 7435, 2304, 3615, 9957, 21136, 37440, 25894, 24793, 4032, 2288, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224, 8978, 9673, 5016, 46958, 25720, 9673, 46958, 10632, 6055, 4117, 19528, 8315, 18871, 5551, 3224, 28259, 5172, 23032, 16572, 4587, 6055, 1863, 19913, 32640, 9673, 7435, 39237, 3615], "avg_logprob": -0.06321022847686152, "compression_ratio": 1.946524064171123, 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new file mode 100644
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18714, "start": 165.42, "end": 187.14, "text": "و كذلك الامتحان النهائي كذلك هي في توزيع الدرجات موزعة يعني مبين أيضا توزيعها في نهاية الخطة يعني توزيع الدرجات عندنا موزعة ما بين", "tokens": [2407, 9122, 8848, 23275, 2423, 10943, 2655, 5016, 7649, 28239, 3224, 16373, 1829, 9122, 8848, 23275, 39896, 8978, 33427, 11622, 40228, 32748, 47341, 9307, 3714, 2407, 11622, 27884, 37495, 22653, 3714, 3555, 9957, 36632, 11242, 995, 33427, 11622, 40228, 11296, 8978, 8717, 11296, 10632, 33962, 9566, 3660, 37495, 22653, 33427, 11622, 40228, 32748, 47341, 9307, 43242, 8315, 3714, 2407, 11622, 27884, 19446, 49374], "avg_logprob": -0.09661865048110485, "compression_ratio": 1.880952380952381, "no_speech_prob": 5.364418029785156e-07, "words": [{"start": 165.42, "end": 165.74, "word": "و", "probability": 0.9775390625}, {"start": 165.74, "end": 166.42, "word": " كذلك", "probability": 0.8468424479166666}, {"start": 166.42, "end": 167.12, "word": " الامتحان", "probability": 0.93359375}, {"start": 167.12, 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والمشاركات أيضًا في المحاضرة عندما نسأل وعندما نطلب من الطالب أن تشارك ونسأل وتجيب الطالب على سؤالنا أيضًا في عندنا مراجع كذلك مراجع المساق مبينة في الخطة هذا كل ما يتعلق بخطة المساق", "tokens": [2304, 16095, 21542, 11242, 13063, 16070, 2304, 8592, 9640, 4117, 9307, 36632, 11242, 14111, 995, 8978, 9673, 5016, 46958, 25720, 43242, 15042, 8717, 3794, 10721, 1211, 4032, 3615, 41260, 15042, 8717, 9566, 46152, 9154, 41950, 6027, 3555, 14739, 6055, 8592, 9640, 4117, 4032, 1863, 3794, 10721, 1211, 34683, 7435, 1829, 3555, 41950, 6027, 3555, 15844, 8608, 33604, 6027, 8315, 36632, 11242, 14111, 995, 8978, 43242, 8315, 3714, 2288, 26108, 3615, 9122, 8848, 23275, 3714, 2288, 26108, 3615, 9673, 3794, 995, 4587, 3714, 3555, 9957, 3660, 8978, 33962, 9566, 3660, 23758, 28242, 19446, 7251, 2655, 30241, 4587, 4724, 9778, 9566, 3660, 9673, 3794, 995, 4587], "avg_logprob": -0.08311012245359875, "compression_ratio": 1.9887005649717515, "no_speech_prob": 8.344650268554688e-07, "words": [{"start": 208.12, 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فالسلام", "probability": 0.938720703125}, {"start": 1584.53, "end": 1585.01, "word": " عليكم", "probability": 0.6702880859375}, {"start": 1585.01, "end": 1585.57, "word": " ورحمة", "probability": 0.85687255859375}, {"start": 1585.57, "end": 1585.83, "word": " الله", "probability": 0.98046875}, {"start": 1585.83, "end": 1586.55, "word": " وبركاته", "probability": 0.98564453125}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 1587.083875, "duration_after_vad": 1465.9924999999967}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZ8bh257eMcqZ22bPBOhO8z/fQmm6TAt2n8_raw.srt b/PL9fwy3NUQKwZ8bh257eMcqZ22bPBOhO8z/fQmm6TAt2n8_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..75d469fa625fafcdfd864fce3c7b329f9d56ddb1
--- /dev/null
+++ b/PL9fwy3NUQKwZ8bh257eMcqZ22bPBOhO8z/fQmm6TAt2n8_raw.srt
@@ -0,0 +1,1744 @@
+1
+00:00:22,070 --> 00:00:26,290
+بسم الله الرحمن الرحيم الحمد لله رب العالمين
+
+2
+00:00:26,290 --> 00:00:30,390
+والصلاة والسلام على أشرف المرسلين سيدنا محمد وعلى
+
+3
+00:00:30,390 --> 00:00:35,790
+آله وصحبه أجمعين بنات الطالبات السلام عليكم ورحمة
+
+4
+00:00:35,790 --> 00:00:41,970
+الله وبركاته في المحاضرة السابقة تحدثنا عن أناط
+
+5
+00:00:41,970 --> 00:00:49,170
+القيادة وتحدثنا عن القيادة الديمقراطية والقيادة
+
+6
+00:00:49,170 --> 00:00:54,180
+الدكتاتوريةوتحدثنا عن القيادة التقليدية اللي هي
+
+7
+00:00:54,180 --> 00:01:00,380
+الفيروقراطية والقيادة المشاغبة أو المتمردة في هذه
+
+8
+00:01:00,380 --> 00:01:07,700
+المحاضرة سنتحدث بإذن الله تعالى عن أنماط أو عن
+
+9
+00:01:07,700 --> 00:01:14,920
+اختيار القادة وتدريبهموسنعرف كيف نختار القادة وكيف
+
+10
+00:01:14,920 --> 00:01:19,600
+نقوم بتدريبهم طرق التدريب اللازمة له قبل ذلك
+
+11
+00:01:19,600 --> 00:01:26,300
+سنتحدث عن أنواع القادة في طريق تنظيم المجتمع طبعا
+
+12
+00:01:26,300 --> 00:01:31,880
+أنواع القادة عندنا أنواع في أنواع من القادة حسب
+
+13
+00:01:31,880 --> 00:01:37,620
+السلطة التي يمتلكونها وحسب النفوذ الذي يمتلكونه
+
+14
+00:01:37,620 --> 00:01:44,200
+وأيضا في قادة حسب الثرواتالتي يمتلكونها هذه أنواع
+
+15
+00:01:44,200 --> 00:01:49,780
+من القادة داخل المجتمع وأيضا في قادة بحكم مناصبهم
+
+16
+00:01:49,780 --> 00:01:57,140
+الإدارية التي يعني يكونون فيها وأيضا في قادة بحكم
+
+17
+00:01:57,140 --> 00:02:02,340
+الخبرة والتخصص هذا ما نقول عنه قائد بحكم أنه متخصص
+
+18
+00:02:02,340 --> 00:02:09,220
+وخبير في تخصصه فيعتبر قائد في هذا التخصصوغير ذلك
+
+19
+00:02:09,220 --> 00:02:15,850
+هؤلاء وغيرهم هذه أنواع القادة داخل المجتمعطبعا
+
+20
+00:02:15,850 --> 00:02:19,830
+بجانب هذه الأنواع اللي احنا ذكرناها وغيرها يوجد
+
+21
+00:02:19,830 --> 00:02:24,230
+نوع عندنا اسمه القادة الشعبيين وهذه يعتبروا من أهم
+
+22
+00:02:24,230 --> 00:02:28,170
+أنواع القادة الموجودين في المجتمع الذين يمثلون
+
+23
+00:02:28,170 --> 00:02:32,730
+المجتمع وهم القادة الشعبيين داخل المجتمع وهذا هو
+
+24
+00:02:32,730 --> 00:02:36,350
+الذي يعني الأخصائي الاجتماعي أو المنظم الاجتماعي
+
+25
+00:02:36,350 --> 00:02:41,360
+يتعامل معهم داخل المجتمعلماذا؟ لأن لهم تأثير قوي
+
+26
+00:02:41,360 --> 00:02:45,340
+على أفراد المجتمع لأن هذه القادة الشعبيين نابعين
+
+27
+00:02:45,340 --> 00:02:49,600
+من المجتمع ينبعون من المجتمع ويختارهم المجتمع
+
+28
+00:02:49,600 --> 00:02:54,880
+ويعرفون المجتمع معرفة جيدة فلذلك حلقة الوصل ما بين
+
+29
+00:02:54,880 --> 00:02:58,660
+الأقصاء الاجتماعي أو المنظم الاجتماعي وما بين
+
+30
+00:02:58,660 --> 00:03:03,800
+أفراد المجتمع ما يريد أن يوصله الأقصاء الاجتماعي
+
+31
+00:03:03,800 --> 00:03:08,000
+للمجتمع يكون عن طريق هؤلاء القادةالقادة الشعبيين
+
+32
+00:03:08,000 --> 00:03:12,500
+داخل المجتمع وكذلك إذا أراد المجتمع أن يبلغ الجهة
+
+33
+00:03:12,500 --> 00:03:15,960
+المهنية، المنظم الاجتماعي أو الخصائص الاجتماعي،
+
+34
+00:03:15,960 --> 00:03:20,100
+أيضًا عن طريق هؤلاء القادة، يبلغونهم، فلذلك هم
+
+35
+00:03:20,100 --> 00:03:25,020
+حلقة الوصل ما بين المنظم الاجتماعي وأيضًا ما بين
+
+36
+00:03:25,020 --> 00:03:30,580
+المجتمع فلذلك هؤلاء القادة الشعبيين كيف نكتشفهم؟
+
+37
+00:03:31,190 --> 00:03:35,470
+يتم اكتشافهم عن طريق الاحتكاك المباشر بالمجتمع
+
+38
+00:03:35,470 --> 00:03:39,730
+وعلى الأخصائي أو المنظم الاجتماعي أن يكتشف هؤلاء
+
+39
+00:03:39,730 --> 00:03:45,530
+القادة داخل المجتمع ويقوم بتسجيلهم ومعرفة أنواعهم
+
+40
+00:03:45,530 --> 00:03:50,840
+ومعرفة خبراتهم ومعرفة ممد تأثيرهم في المجتمعفلذلك
+
+41
+00:03:50,840 --> 00:03:55,620
+من هنا يعني من خلال الاحتكاك الأخصائي يستطيع
+
+42
+00:03:55,620 --> 00:03:59,500
+المستمر داخل المجتمع يستطيع أن يكتشف هؤلاء القاد
+
+43
+00:03:59,500 --> 00:04:05,860
+الشعبيين المؤثرين داخل المجتمع طبعا
+
+44
+00:04:05,860 --> 00:04:11,920
+بعد ما يتم اكتشاف هؤلاء داخل المجتمع لابد أن نقوم
+
+45
+00:04:11,920 --> 00:04:19,050
+باختيارهم كيف يتم اختيار هؤلاء القادةالقادة داخل
+
+46
+00:04:19,050 --> 00:04:23,770
+المجتمع كيف يتم اختيارهم من قبل المنظم الاجتماعي
+
+47
+00:04:23,770 --> 00:04:28,410
+طبعا لكي يتم الاختيار بصورة موضوعية وليست بصورة
+
+48
+00:04:28,410 --> 00:04:33,410
+عشوائية ولا صورة فيها تحيز أو غير ذلك ولذلك بنقول
+
+49
+00:04:33,410 --> 00:04:40,010
+تخير او اختيار موضوعي للقادة طبعا هنا بتتم عن طريق
+
+50
+00:04:40,010 --> 00:04:44,130
+الدراسة دراسة العمليات المطلوبة لها القيادة ما هي
+
+51
+00:04:44,130 --> 00:04:47,750
+العمليات المطلوبة لها القيادةطبعا احنا سجلنا
+
+52
+00:04:47,750 --> 00:04:52,410
+القادة هنا بدنا ندرس اللي هي العمليات المطلوبة
+
+53
+00:04:52,410 --> 00:04:57,610
+لإنهم يعني يكونوا قادة ويقوموا بالعمليات اللازمة
+
+54
+00:04:57,610 --> 00:05:03,470
+منهم ولذلك من هنا يعني أحياناالعمليات المطلوبة من
+
+55
+00:05:03,470 --> 00:05:06,990
+قائد معين تختلف عن العمليات المطلوبة من قائد آخر
+
+56
+00:05:06,990 --> 00:05:12,230
+فمثلا لو كان رئيس لمجلس محلي بلدي يعني هذا هو
+
+57
+00:05:12,230 --> 00:05:15,630
+المطلوب أن يكون له قائد على مستوى قرية أو مدينة
+
+58
+00:05:15,630 --> 00:05:21,460
+يختلف عن رئيس لحملة جمع المالفيه صفات معينة تختلف
+
+59
+00:05:21,460 --> 00:05:24,740
+رئيس المجلس المحلي على مستوى القرية أو المدينة
+
+60
+00:05:24,740 --> 00:05:29,720
+يختلف عن رئيس لحملة جمع المال لأن رئيس حملة جمع
+
+61
+00:05:29,720 --> 00:05:34,220
+المال له صفات معينة أيضا وله خبرات وله تخصص معين
+
+62
+00:05:34,220 --> 00:05:39,460
+لفيه جمع الأموال يختلف عن رئيس مجلس محلي يعني له
+
+63
+00:05:39,460 --> 00:05:43,720
+صفات إدارية أيضا يستطيع القيام بها فلذلك من هنا
+
+64
+00:05:43,720 --> 00:05:51,010
+تختلف القيادة حسب العملية المطلوبة لهاالقائد فلذلك
+
+65
+00:05:51,010 --> 00:05:55,030
+من هنا بنقول لابد أن ندرس العمليات المطلوبة لها
+
+66
+00:05:55,030 --> 00:05:59,390
+القيادة دراسة جيدة ومعرفة طبيعة هذه العمليات
+
+67
+00:05:59,390 --> 00:06:04,470
+الناحية الثانية مطلوب دراسة نوع الجماعة المطلوب
+
+68
+00:06:04,470 --> 00:06:10,150
+قيادتها ما هي هذه الجماعة؟ هل هي جماعة من الذكور؟
+
+69
+00:06:10,150 --> 00:06:13,490
+من الإناس؟ كم أعمارهم؟ هل هما جماعة الأطفال أو
+
+70
+00:06:13,490 --> 00:06:17,290
+جماعة الشباب؟ هل هما جماعة الأسوياء أو غير
+
+71
+00:06:17,290 --> 00:06:23,230
+الأسوياء؟واضح؟ هل جماعة مؤهلة تأهيلا علميا أم
+
+72
+00:06:23,230 --> 00:06:27,890
+جماعة متوسطة التأهيل العلمى؟ هذه كلها دراسة
+
+73
+00:06:27,890 --> 00:06:31,570
+الجماعات وأنواع الجماعات حتى نختار لها القيادة
+
+74
+00:06:31,570 --> 00:06:37,710
+الملائمة والمناسبة هذه هي الخطوة الثانية في اختيار
+
+75
+00:06:37,710 --> 00:06:41,410
+القائد لابد أن ندرس نوع الجماعة المطلوب قيادتها
+
+76
+00:06:41,410 --> 00:06:46,050
+دراسة مستفيدة ودراسة علمية حتى نختار لها القائد
+
+77
+00:06:46,050 --> 00:06:51,340
+الملائم والمناسبنعم يعني نوع الفئة طبعا نوع الفئة
+
+78
+00:06:51,340 --> 00:06:56,840
+ونوع التخصص ونوع السن المرحلة السنية وهل مستواهم
+
+79
+00:06:56,840 --> 00:07:00,260
+متعلمين او غير متعلمين مستواهم الثقافة ومستواهم
+
+80
+00:07:00,260 --> 00:07:06,520
+التعليمي وهكذا هذه تختلف المستوى العمري لهم ايضا
+
+81
+00:07:06,520 --> 00:07:11,280
+هكذا اذا على حسب هذه المقاييس ممكن نختار نوعية
+
+82
+00:07:11,280 --> 00:07:13,900
+الجماعة المطلوب لها القيادة
+
+83
+00:07:16,030 --> 00:07:19,230
+كل نوع من هذه الجماعات اللي احنا ذكرناها بكون لها
+
+84
+00:07:19,230 --> 00:07:25,590
+تحتاج إلى نوعية معينة من القادة الخطوة الثالثة في
+
+85
+00:07:25,590 --> 00:07:29,590
+اختيار القادة لابد ان ندرس القيادة المتوفرة
+
+86
+00:07:29,590 --> 00:07:33,890
+الموجودة درسنا العمليات ودرسنا الجماعات المطلوب
+
+87
+00:07:33,890 --> 00:07:37,870
+لها قيادة هنا لازم ندرس القادة ندرس القيادة
+
+88
+00:07:37,870 --> 00:07:42,110
+المتوفرة بالعملية المطلوبة مع الجماعة المطلوبة
+
+89
+00:07:42,560 --> 00:07:47,200
+المعينة اللي هي المطلوبة يعني فلذلك من هنا لابد أن
+
+90
+00:07:47,200 --> 00:07:50,120
+نختار القائد الملائم أو القيادة الملائمة أو
+
+91
+00:07:50,120 --> 00:07:55,460
+المناسبة للعملية الملائمة وللجماعة الملائمة معاه
+
+92
+00:07:56,260 --> 00:08:00,560
+واضح هذه الخطوة الثالثة لازم نحصر أسماء القادة
+
+93
+00:08:00,560 --> 00:08:04,980
+وبعد ذلك نوزعهم على اللي هي العملية المطلوبة مع
+
+94
+00:08:04,980 --> 00:08:09,320
+الجماعة المعينة فالمنظم هنا بيحصر مجموعة الجماعات
+
+95
+00:08:09,320 --> 00:08:14,380
+والعمليات المطلوب قيدتها ثم يصف القادة حسب يصنف
+
+96
+00:08:14,380 --> 00:08:20,340
+القادة حسب صلاحيتهم حسب صلاحيتهم لقيادة هذه
+
+97
+00:08:20,340 --> 00:08:24,880
+الجماعات وحسب نوع العمليات المطلوبة مع هذه
+
+98
+00:08:24,880 --> 00:08:30,280
+الجماعاتواضح؟ فهنا بيختار أفضل العناصر لقيادة هذه
+
+99
+00:08:30,280 --> 00:08:34,700
+الجماعات وقيادة العمليات المختلفة داخل المجتمع
+
+100
+00:08:34,700 --> 00:08:42,120
+عرفنا كيف اختيار القادة والقيادة؟ خلاص اخترنا الآن
+
+101
+00:08:42,120 --> 00:08:47,280
+القيادة الملائمة للجماعة الملائمة حسب العمليات
+
+102
+00:08:47,280 --> 00:08:49,400
+المناسبة لهذه الجماعة
+
+103
+00:08:52,020 --> 00:08:57,120
+الان طبعا لابد من أن نقوم بعملية التدريب لهؤلاء
+
+104
+00:08:57,120 --> 00:09:02,190
+القادة الذين تم اختيارهملابد أن نعمل لهم حمالات
+
+105
+00:09:02,190 --> 00:09:06,310
+تدريبية ودورات تدريبية حتى يستطيعوا القياد
+
+106
+00:09:06,310 --> 00:09:12,470
+بواجباتهم ومسؤولياتهم مع الجماعات المختلفة
+
+107
+00:09:12,470 --> 00:09:17,410
+بالعمليات المختلفة حسب نوع العملية ونوع الجماعة
+
+108
+00:09:17,410 --> 00:09:21,850
+التي تم اختيار القائد لها أو القيادة لها فلذلك من
+
+109
+00:09:21,850 --> 00:09:25,890
+هنا لابد أن نقوم بعمليات التدريب طبعا التدريب
+
+110
+00:09:25,890 --> 00:09:31,880
+لماذا؟حتى نستطيع أن نغرس في هذا المتدرب يعني مزيد
+
+111
+00:09:31,880 --> 00:09:36,600
+من المهارة مزيد من الخبرة مزيد من كيفية التعامل مع
+
+112
+00:09:36,600 --> 00:09:41,900
+الجامعات وكيفية التصرف في العمليات المختلفة لهذه
+
+113
+00:09:41,900 --> 00:09:45,660
+الجامعات فلذلك من هنا لابد من تدريب القائد حتى
+
+114
+00:09:45,660 --> 00:09:51,620
+يتعلم ويقوم بمارس دوره كقائد مع هذه الجامعات
+
+115
+00:09:51,620 --> 00:09:58,290
+المختلفة فلذلك من هنالابد هنا أن نختار برامج
+
+116
+00:09:58,290 --> 00:10:02,990
+تدريبية معينة لهؤلاء القادة حسب نوعياتهم وخبراتهم
+
+117
+00:10:02,990 --> 00:10:07,410
+ومؤهلاتهم فلذلك من هنا لابد من أن تكون برامج
+
+118
+00:10:07,410 --> 00:10:11,170
+التدريب تركز على التالي على ماذا تركز برامج
+
+119
+00:10:11,170 --> 00:10:16,330
+التدريب المختارة لهؤلاء القادة تركز على إمداد
+
+120
+00:10:16,330 --> 00:10:24,110
+المتدرب أول خطوة بالمعلومات المعلومات المطلوبةما
+
+121
+00:10:24,110 --> 00:10:28,990
+هي المعلومات المطلوبة للقائد لأن المعلومات أهم شيء
+
+122
+00:10:28,990 --> 00:10:33,030
+للقائد لابد أن يعرف ويتعرف عن المعلومات حول
+
+123
+00:10:33,030 --> 00:10:37,670
+القيادة وكيفية التصرف أيضا الناحية الثانية إكساب
+
+124
+00:10:37,670 --> 00:10:44,870
+المتدرب اتجاهات إيجابية للقيام بمسؤوليته ثالثا
+
+125
+00:10:44,870 --> 00:10:51,250
+إكساب المتدرب المهارات اللازمة المهارة في كيفية
+
+126
+00:10:51,250 --> 00:10:58,990
+القيام بواجباتهكقائد في المجتمع واضح كل هذه الأمور
+
+127
+00:10:58,990 --> 00:11:04,070
+الثلاثة المعلومات والاتجاهات الإيجابية والمهارات
+
+128
+00:11:04,070 --> 00:11:09,370
+لابد أن نكسبها لمن؟ للقائدة في المجتمع حتى يستطيع
+
+129
+00:11:09,370 --> 00:11:14,750
+القيام بمهامهم نأخذ النقطة الأولى مداد المتدرب
+
+130
+00:11:14,750 --> 00:11:20,360
+بالمعلومات كيف نمد المتدرب بالمعلومات؟القائد
+
+131
+00:11:20,360 --> 00:11:27,640
+المتدرب كيف نمده بالمعلومات هنا طبعا عندنا لابد أن
+
+132
+00:11:27,640 --> 00:11:31,980
+يلم أولا بالمعلومات حول السلوك الإنساني نريد أن
+
+133
+00:11:31,980 --> 00:11:37,660
+نعرف كيف يتعامل مع الإنسان وسلوك الإنسان ويتصرف في
+
+134
+00:11:37,660 --> 00:11:41,620
+المواقف المختلفة الإنسانية فلذلك لابد أن يفهم
+
+135
+00:11:41,620 --> 00:11:46,490
+الدوافع الإنسانية دوافع هذا الإنسانحتى يستطيع
+
+136
+00:11:46,490 --> 00:11:50,550
+التعامل مع هذا الإنسان هذه الناحية الأولى السلوك
+
+137
+00:11:50,550 --> 00:11:55,330
+الإنساني ودوافعه يتعرف على الحياة الدفاعية القيادة
+
+138
+00:11:55,330 --> 00:11:59,890
+ديناميات الجماعة أيضا كيفية استخدام هذه المعلومات
+
+139
+00:11:59,890 --> 00:12:07,340
+في عمله مع المجتمع كيف يستطيعون استخدامها؟أيضا
+
+140
+00:12:07,340 --> 00:12:12,920
+لابد من ناحية ثانية أن يتزود بمعارف بحتية، مش بس
+
+141
+00:12:12,920 --> 00:12:17,380
+معلومات و معارف نظرية، أيضا معارف بحتية، كيف يقوم
+
+142
+00:12:17,380 --> 00:12:22,330
+بالبحث و الدراسة المهنية عن طريق البحثلأن البحث هو
+
+143
+00:12:22,330 --> 00:12:25,870
+الذي يوصلنا إلى الحقيقة فلذلك لابد أن نعرف هؤلاء
+
+144
+00:12:25,870 --> 00:12:30,610
+القادة على طرق البحث العلمي وكيف يصل إلى الحقيقة
+
+145
+00:12:30,610 --> 00:12:35,390
+ويعرف المشكلة عن طريق البحث العلمي فلابد أن نزودهم
+
+146
+00:12:35,390 --> 00:12:39,910
+بالمعارف البحتية اللي بتجعله قادر على عمل البحوث
+
+147
+00:12:39,910 --> 00:12:44,570
+ويستخدم هذه .. ويستخدمها في عمله أو يحيانا يستعيل
+
+148
+00:12:44,570 --> 00:12:50,580
+بالخبراءليجراء هذه البحوث يستعين بالخبراء في مجال
+
+149
+00:12:50,580 --> 00:12:55,660
+اللي هو البحث واضح لماذا؟ حتى نكسب هذا المتدرب
+
+150
+00:12:55,660 --> 00:13:00,520
+اللي هو القائد المتدرب الأسلوب العلمي في عمله ..
+
+151
+00:13:00,520 --> 00:13:05,170
+الأسلوب العلمييتعلم الإسلوب العلمي في كيفية
+
+152
+00:13:05,170 --> 00:13:09,610
+التعامل في معرفة مشكلات المجتمع عن طريق البحث
+
+153
+00:13:09,610 --> 00:13:13,530
+العلمي و يستعيد بالخبراء في المجتمع حتى يقوم
+
+154
+00:13:13,530 --> 00:13:17,930
+بدراسة المشاكل دراسة علمية و ليست دراسة عشوائية
+
+155
+00:13:17,930 --> 00:13:21,970
+هكذا، لأ تعتمد على المنهج العلمي و الإسلوب العلمي
+
+156
+00:13:21,970 --> 00:13:30,410
+أيضا من ناحيةخلصنا اذا لابد ان يتعلم في المعلومات
+
+157
+00:13:30,410 --> 00:13:35,930
+هذا القائد الجوانب المعلوماتية نزوده بالمعارف
+
+158
+00:13:35,930 --> 00:13:41,040
+النظرية الناحية التانية بالمعارف البحثيةواضح اللتي
+
+159
+00:13:41,040 --> 00:13:45,880
+يعتمد فيها القائد على كيفية دراسة المشكلة أو معرفة
+
+160
+00:13:45,880 --> 00:13:50,140
+الحقيقة عن طريق الأسلوب العلمي اللي هو البحث وإذا
+
+161
+00:13:50,140 --> 00:13:54,480
+لم يعرف نعلرله المبادئ الأولية وكيفية الاستعارة
+
+162
+00:13:54,480 --> 00:13:59,480
+بالخبراء والمختصين في دراسة مشكلات المجتمع هذا
+
+163
+00:13:59,480 --> 00:14:03,740
+الجانب المتعلق بالجوانب اللي هي المعلومات وكيف
+
+164
+00:14:03,740 --> 00:14:09,530
+نكسب المعلومات إلى القائدالناحية الثانية لابد أن
+
+165
+00:14:09,530 --> 00:14:13,930
+نقوم بتدريبه عن طريق الاتجاهات المطلوب إكسابها
+
+166
+00:14:13,930 --> 00:14:18,050
+للمتدرب، في إتجاهات إيجابية لابد أن يعلم بها
+
+167
+00:14:18,050 --> 00:14:23,770
+ويعرفها المتدرب من القادة فالاتجاهات العامة يجب أن
+
+168
+00:14:23,770 --> 00:14:30,130
+يحرص أي برنامج تدريبي على إكسابه والتأكدعليها وأهم
+
+169
+00:14:30,130 --> 00:14:33,890
+هذه الاتجاهات العامة ما هي الاتجاهات الإيجابية
+
+170
+00:14:33,890 --> 00:14:38,490
+المطلوب إكسابها للقائد المتدرب ما هي هذه
+
+171
+00:14:38,490 --> 00:14:41,450
+الاتجاهات؟ كل طالب يبدأ تحكي لاتجاه معين، أيه
+
+172
+00:14:41,450 --> 00:14:44,010
+ببنتي؟ الإيمان بالمهمة التي يقوم بها الإيمان
+
+173
+00:14:44,010 --> 00:14:47,990
+بالمهمة التي يقوم بها، إذا لابد أن يؤمن بعمله،
+
+174
+00:14:47,990 --> 00:14:53,250
+لابد أن نعرفه كيف يؤمن بدوره كقائد في المجتمع، أيه
+
+175
+00:14:53,250 --> 00:14:53,930
+يا بنتي؟
+
+176
+00:14:58,930 --> 00:15:03,890
+إيمان بقدرة الآخرين على التفكير والعمل ولذلك من
+
+177
+00:15:03,890 --> 00:15:07,250
+هنا لابد أن نؤمن بقدرات الآخرين و الله نستهيل بهم
+
+178
+00:15:07,250 --> 00:15:12,690
+طيب كيف لابد أن نعلم هذا القائد بأن يؤمن بقدرات
+
+179
+00:15:12,690 --> 00:15:17,190
+الآخرين الذين يقودهم أو يتصل بهم في المجتمع بأن كل
+
+180
+00:15:17,190 --> 00:15:23,550
+إنسان لديه قدرة لديه إمكانية لديه طاقة لديه موهبة
+
+181
+00:15:23,550 --> 00:15:29,810
+لديه مهارة الإنسان مخلوق ليس عبثافلذلك لابد أن
+
+182
+00:15:29,810 --> 00:15:33,570
+نؤمن بهذه القدرات و نحن في الخدمة الاجتماعية نؤمن
+
+183
+00:15:33,570 --> 00:15:38,050
+بالقدرات الإنسانية، أي إنسان
+
+184
+00:15:44,100 --> 00:15:47,160
+احنا قولنا اغلقوا الجوالات لو سمحتوا، اغلقوا كل
+
+185
+00:15:47,160 --> 00:15:50,460
+الجوالات، دي جات متأخرة هدف، راجعة أي طالبة ماحة
+
+186
+00:15:50,460 --> 00:16:02,480
+جوال، تضعوا silent أو تغلقوا، زي ما فعلتنا خير،
+
+187
+00:16:02,480 --> 00:16:07,940
+تفضلي يا بنتفضلوا يالا بس الباقي الجوالات تغلق لو
+
+188
+00:16:07,940 --> 00:16:11,800
+سمحتوا يعني هالفترة الباقية يعني نصف ساعة او اقل
+
+189
+00:16:11,800 --> 00:16:15,560
+بدنا نغلق الجوال ومعليش يعني اللي تتصل علينا بعدين
+
+190
+00:16:15,560 --> 00:16:19,520
+بتتصل علينا ماشي نستمر
+
+191
+00:16:23,830 --> 00:16:27,930
+الاتجاهات المطلوب اكسبها قلنا أول شيء الإيمان
+
+192
+00:16:27,930 --> 00:16:30,710
+بقدرة الآخرين اللي هي الإيمان بالمهمة اللتي يقوم
+
+193
+00:16:30,710 --> 00:16:35,410
+بها القائد النحية الثانية الإيمان بقدرات الآخرين
+
+194
+00:16:35,410 --> 00:16:39,570
+اللي هي قدرتهم على التفكير والعمل المنتج وتحمل
+
+195
+00:16:39,570 --> 00:16:44,810
+المسئوليات والقيادة أيضا لأن القائد هو مساعد ومعين
+
+196
+00:16:44,810 --> 00:16:51,410
+لأفراد المجتمع فلذلكلابد أن نكسب هذه الاتجاهات
+
+197
+00:16:51,410 --> 00:16:55,550
+اللي هو الإيمان بقدرة الآخرين لأن الآخرين لديهم
+
+198
+00:16:55,550 --> 00:16:59,570
+القدرة على العطاء الآخرين لديهم القدرة على الإنتاج
+
+199
+00:16:59,570 --> 00:17:04,610
+الآخرين لديهم القدرة و لديهم المهارة و لديهم
+
+200
+00:17:04,610 --> 00:17:08,710
+القدرة في تحمل المسؤوليات أيضًا هذا اللي هي من
+
+201
+00:17:08,710 --> 00:17:12,570
+الاتجاهات المطلوب إكسابها للمتدرب ماذا أيضًا من
+
+202
+00:17:12,570 --> 00:17:14,990
+الاتجاهات المطلوب إكسابها للمتدرب؟ ايه يا بنت؟
+
+203
+00:17:14,990 --> 00:17:19,670
+احترام آراء غير وحدهماحترام الرأي الغير وهذا مهم
+
+204
+00:17:19,670 --> 00:17:24,830
+جدا لا نفرض رأينا على الغير لابد أن ننصف جيدا و
+
+205
+00:17:24,830 --> 00:17:32,350
+نستمع جيدا للآخرين و نعرف ماذا يقول الآخر فهنا
+
+206
+00:17:32,350 --> 00:17:36,130
+لابد أن نعطيه نوع من الاحترام و التقدير لرأيه مهما
+
+207
+00:17:36,130 --> 00:17:41,050
+كان هذا الرأي و لابد أن دائما في مناقشتنا أو نعود
+
+208
+00:17:41,050 --> 00:17:45,300
+القائدفي طريق تنظيم المجتمع عندما نعطيه الدورات
+
+209
+00:17:45,300 --> 00:17:52,440
+التدريبية أن اختلاف الرأي لا يفسد للود قضية بيعطي
+
+210
+00:17:52,440 --> 00:17:59,440
+مهارة أكتر واختلاف الأراء يوصلنا
+
+211
+00:17:59,440 --> 00:18:05,730
+إلى نتائج إيجابية وإلى الحقيقةدائما لابد هذا
+
+212
+00:18:05,730 --> 00:18:09,790
+القائد أن نغرس فيه هذه الصفة صفة احترام الآخرين
+
+213
+00:18:09,790 --> 00:18:14,390
+واحترام رأي الآخرين هذه في الدورات التدريبية نركز
+
+214
+00:18:14,390 --> 00:18:18,490
+عليها أيضا لقادة المجتمع بأن يحترم أفراد المجتمع
+
+215
+00:18:18,490 --> 00:18:23,590
+ويحترم آرائهم مهما كانت هذه الآراء و هذه وجهات
+
+216
+00:18:23,590 --> 00:18:28,690
+النظر لابد أن نحترمها الناحية الثانية الأخرى ..
+
+217
+00:18:28,690 --> 00:18:35,180
+أيوة بنتيالقدرة على التفكير أيضًا القدرة على
+
+218
+00:18:35,180 --> 00:18:38,560
+التفكير هذه لابد أن نغرس هذا الاتجاه لدلقائق
+
+219
+00:18:38,560 --> 00:18:43,500
+القدرة على التفكير الموضوعي الواقعي ليس التفكير
+
+220
+00:18:43,500 --> 00:18:47,780
+الخيالي بل لابد أن يكون تفكيره واقعيا وفي نفس
+
+221
+00:18:47,780 --> 00:18:53,220
+الوقت موضوعيا واضح؟ هذه أيضًا من الجوانب اللي هي
+
+222
+00:18:53,220 --> 00:18:57,140
+الاتجاهات الإيجابية التي لابد من غرسها أيوة يا
+
+223
+00:18:57,140 --> 00:18:57,500
+بنتي
+
+224
+00:19:01,150 --> 00:19:06,550
+القدرة على التحكم في النزاعات الشخصية في النزاعات
+
+225
+00:19:06,550 --> 00:19:14,230
+الشخصية طبعا هذه أيضا النزاعات النزاعات وليست
+
+226
+00:19:14,230 --> 00:19:20,110
+النزاعات النزاعات يعني أحيان نعم رغبات وسمات
+
+227
+00:19:20,110 --> 00:19:24,630
+وخصائص أمور معينة تكون ذات نزع عند هذا الإنسان
+
+228
+00:19:24,630 --> 00:19:29,090
+فهنا لابد أن نعلمه كيفية التحكم في هذه النزاعات
+
+229
+00:19:29,090 --> 00:19:34,590
+الشخصيةودوافعه الشخصية وقال لا تطغى النزاعات
+
+230
+00:19:34,590 --> 00:19:41,370
+الشخصية والدوافع الشخصية على حاجات المجتمع وقال لا
+
+231
+00:19:41,370 --> 00:19:45,730
+يحقق نزاعاته الشخصية على حساب المجتمع هذه أيضا
+
+232
+00:19:45,730 --> 00:19:52,670
+لابد أن نغرسها لدى القائد في تنظيم المجتمع أيوا
+
+233
+00:19:52,670 --> 00:19:57,330
+هنا مين بتتكلم؟ ماتكلمتش؟ مين ماتكلمتش؟ طيب
+
+234
+00:19:57,330 --> 00:20:03,550
+ماتكلمتش؟ أنتي يلا تفضليالرغبة في النمو المهني
+
+235
+00:20:03,550 --> 00:20:07,530
+والتعلم أيضا نغرسها وهذا اتجاه لدى القائد اللى هو
+
+236
+00:20:07,530 --> 00:20:11,810
+بأن نغرس فيه دائما الرغبة في النمو المهني والتعلم
+
+237
+00:20:11,810 --> 00:20:16,290
+دائما كل يوم يتعلم شيء جديد لابد من التعلم في
+
+238
+00:20:16,290 --> 00:20:21,810
+أشياء جديدة هذه الاتجاهات الإيجابية التي يجب غرسها
+
+239
+00:20:21,810 --> 00:20:27,550
+لدى القائد في تنظيم المجتمع عندما نقوم بتدريبه
+
+240
+00:20:27,550 --> 00:20:33,350
+وعقد الدورات التدريبية له ماذا أيضافيه برضه من ال
+
+241
+00:20:33,350 --> 00:20:37,370
+.. خلصنا الاتجاهات الإيجابية، فيه المهارات ما هي
+
+242
+00:20:37,370 --> 00:20:44,050
+المهارات اللازمة غرسها لدى أو .. يعني تنميتها لدى
+
+243
+00:20:44,050 --> 00:20:49,170
+القائد الإداري عندما نقوم بالعمليات التدريبية له؟
+
+244
+00:20:49,170 --> 00:20:53,010
+ماذا؟ المهارة في تكوين علاقات منتجة مع رؤسائه و
+
+245
+00:20:53,010 --> 00:20:56,810
+زملائه و تابعينه إذا أول شيء العلاقات، المهارة في
+
+246
+00:20:56,810 --> 00:21:00,310
+تكوين العلاقات و هذا شيء مهم جدا لأن العلاقة مش
+
+247
+00:21:00,310 --> 00:21:05,250
+سهلةالعلاقة تحتاج لمهارة في كيفية تكوين العلاقة مع
+
+248
+00:21:05,250 --> 00:21:10,360
+الآخرينالعلاقة الاجتماعية مع الآخرين، مع زملائهم
+
+249
+00:21:10,360 --> 00:21:14,920
+ومع أفراد مجتمعي ومع مسئولين فهذه لابد أن أغرسها
+
+250
+00:21:14,920 --> 00:21:20,040
+لدى المتدرب في تنظيم المجتمع من القادة المتدربين
+
+251
+00:21:20,040 --> 00:21:25,300
+في تنظيم المجتمع من القادة فلذلك هذه لابد من غرسها
+
+252
+00:21:25,300 --> 00:21:29,800
+اللي هي مهارة في تكوين علاقات منتجة مع الرؤساء مع
+
+253
+00:21:29,800 --> 00:21:33,780
+الزملاء مع التابعين اللي هم الأفراد المجتمع الذين
+
+254
+00:21:33,780 --> 00:21:40,050
+يشرف عليهم ويكون مسئولا عنهمداخل المجتمع ماذا أيضا
+
+255
+00:21:40,050 --> 00:21:43,350
+من المهارات يجب غرس والدة القادة من خلال الدورات
+
+256
+00:21:43,350 --> 00:21:45,110
+التدريبية؟
+
+257
+00:21:51,430 --> 00:21:54,650
+تحسين العلاقات بين التابعين، يعني بين المسؤولين
+
+258
+00:21:54,650 --> 00:21:58,870
+عنهم، ورفع الروح المعنوية بين المسؤول عنهم، يعني
+
+259
+00:21:58,870 --> 00:22:01,890
+واحد قائد مسؤول عن جماعة معينة أو أفراد معينين
+
+260
+00:22:01,890 --> 00:22:05,710
+لابد أن يرفع الروح المعنوية لدى هؤلاء الأفراد،
+
+261
+00:22:05,710 --> 00:22:10,050
+واحنا عارفين يعني دائما في الإدارة أن هناك ارتباط
+
+262
+00:22:10,730 --> 00:22:14,630
+ما بين الروح المعنوية وزيادة العمل أو زيادة
+
+263
+00:22:14,630 --> 00:22:18,070
+الإنتاج كلما زادت الروح المعنوية لدى الأفراد
+
+264
+00:22:18,070 --> 00:22:22,510
+العاملين كلما زادوا الإنتاج وهنا كذلك في العطاء
+
+265
+00:22:22,510 --> 00:22:27,710
+كذلك لابد كلما زادت الروح المعنوية لدى الأفراد
+
+266
+00:22:27,710 --> 00:22:31,670
+التابعين للقائد هنا يزيد انتمائهم لهذا القائد و
+
+267
+00:22:31,670 --> 00:22:36,650
+للوطن وانتمائهم للعطاءفبكون عندهم زيادة في العطار
+
+268
+00:22:36,650 --> 00:22:41,150
+فهذه علاقة ترضية أيضا فهذه مهارة أيضا كذلك رفع
+
+269
+00:22:41,150 --> 00:22:45,770
+الروح المعنوية لدى التابعين لهذا القائد هذه أمور
+
+270
+00:22:45,770 --> 00:22:51,090
+لابد أن نغرسها أيضا لدى القادة عندما نقوم بعمليات
+
+271
+00:22:51,090 --> 00:22:56,450
+التدريب لهم ماذا أيضا؟ استاذ؟ اه ماتكلمتش نهائيا
+
+272
+00:22:56,450 --> 00:23:01,190
+انا انا استاذ مين ماتكلمتش؟ انتي؟ طب يالا كفضل
+
+273
+00:23:07,160 --> 00:23:11,840
+القائد عندما نقوم بتدريب لابد أن نعوده على أن ينقل
+
+274
+00:23:11,840 --> 00:23:18,240
+ما تعلمه وما اكتسبه من خبرة للآخرينكيف ينقل هذه
+
+275
+00:23:18,240 --> 00:23:21,840
+المعلومات التي تعلمها وينقل هذه الخبرات التي
+
+276
+00:23:21,840 --> 00:23:26,380
+اكتسبها إلى الآخرين من المسؤولين من المسؤول عنهم
+
+277
+00:23:26,380 --> 00:23:31,160
+من التابعين له وهكذا هذه أيضا من ضمن اللي هي
+
+278
+00:23:31,160 --> 00:23:36,180
+المهارات التي يجب اكتسبها او اكسبها للقادة من
+
+279
+00:23:36,180 --> 00:23:40,440
+عمليات التدريب في أيضا مهارات أخرى أيوة يا بنتي
+
+280
+00:23:43,880 --> 00:23:47,600
+توزيع المسئوليات على التابعين له والمسئول عنهم
+
+281
+00:23:47,600 --> 00:23:52,300
+توزيع هذه المسئوليات وهذه مهارة أيضا تحتاج لمهارة
+
+282
+00:23:52,300 --> 00:23:56,360
+ومتابعة هذه المسئوليات ليست توزيعها فقط على
+
+283
+00:23:56,360 --> 00:24:00,300
+التابعين له لكن أيضا متابعة هذه المسئوليات هل
+
+284
+00:24:00,300 --> 00:24:03,680
+قاموا بتنفيذها يعني زي وضع الراجل المناسب في
+
+285
+00:24:03,680 --> 00:24:05,440
+المكان المناسب وضع الراجل المناسب في المكان
+
+286
+00:24:05,440 --> 00:24:10,190
+المناسب مع المتابعةعندما يقوم بتحمل هذه المسؤوليات
+
+287
+00:24:10,190 --> 00:24:14,510
+فإذا نعود .. ندرب القائد على توزيع المسؤوليات اللي
+
+288
+00:24:14,510 --> 00:24:18,950
+بتابعينها له ومتابعتهم .. متابعتهم في تنفيذ هذه
+
+289
+00:24:18,950 --> 00:24:24,190
+المسؤوليات واضح؟ ماذا أيضا؟ أيوة يا بنتي؟
+
+290
+00:24:27,130 --> 00:24:32,650
+وهذه مهمة جدا الحوار والنقاش والاجتماع وكيفية دارة
+
+291
+00:24:32,650 --> 00:24:37,510
+الاجتماع وهكذا وهذه مهارة مهارة مهمة جدا كيف
+
+292
+00:24:37,510 --> 00:24:43,210
+القائد في تنظيم المجتمع أن يدير اجتماعا مع عدد من
+
+293
+00:24:43,210 --> 00:24:48,110
+أفراد المجتمعيجتمع معهم ويدير هذا الحوار والنقاش
+
+294
+00:24:48,110 --> 00:24:53,510
+ويتيح الفرصة لكل فرد داخل الاجتماع بالحوار بالنقاش
+
+295
+00:24:53,510 --> 00:24:57,790
+والمحاورة اللي هو الحوار والنقاش والتعبير عن الذات
+
+296
+00:24:57,790 --> 00:25:01,430
+وعن النفس وخاصة في المشكلات التي يطرحونها أو
+
+297
+00:25:01,430 --> 00:25:05,970
+المواضيع التي يطرحونها للنقاش لابد من إعطاء كل فرد
+
+298
+00:25:05,970 --> 00:25:10,950
+في هذه الجماعة بالمناقشة في حرية المناقشة وإبداء
+
+299
+00:25:10,950 --> 00:25:16,220
+رأيه وهذه أيضا تحتاج لمهارةلأن أحيانًا لو نريد أن
+
+300
+00:25:16,220 --> 00:25:20,120
+يسبب العضو في أشياء خارج موضوع المناقشة، لابد أن
+
+301
+00:25:20,120 --> 00:25:24,560
+يحول النقاش إلى الموضوع المراد مناقشته، وهذه مهارة
+
+302
+00:25:24,560 --> 00:25:28,800
+لابد من إكسابها أيضًا للقادة عند القيام بعمليات
+
+303
+00:25:28,800 --> 00:25:36,100
+التدريب لهم ماذا أيضًا؟ نعم يا بنتي المهارة
+
+304
+00:25:36,100 --> 00:25:40,480
+في التعبير والحديث المنطقي المتسلسل والمرتب الحديث
+
+305
+00:25:40,480 --> 00:25:45,360
+والقدرة على الحديث، هذه تعتبر مهارة أيضًالباقة في
+
+306
+00:25:45,360 --> 00:25:49,000
+الحديث واختيار المفردات في اللغة وكيفية توصيل
+
+307
+00:25:49,000 --> 00:25:53,200
+المعلومة باختيار الكلمة الملائمة والمناسبة المراد
+
+308
+00:25:53,200 --> 00:25:58,320
+توصيلها للمستمع هذه مهارة لا يجيدها كل إنسان ولذلك
+
+309
+00:25:58,320 --> 00:26:02,620
+هنا نقوم بعقد دورات التدريبية لهؤلاء القادة
+
+310
+00:26:02,620 --> 00:26:09,990
+لتعويضهم اسلوب الحديثوالتعبير عامة في طبعا خواطرهم
+
+311
+00:26:09,990 --> 00:26:14,430
+حول المواضيع المعينة بكلمات واضحة وتعبير بلغة
+
+312
+00:26:14,430 --> 00:26:20,730
+عربية سليمة وايضا بالنطق السليم للألفاظ عدم تكرار
+
+313
+00:26:20,730 --> 00:26:25,230
+المصطلحات وعدم تكرار المصطلحات ايضا في اجتماع واحد
+
+314
+00:26:25,230 --> 00:26:30,370
+بل ان نذكر المصطلح في مرة واحدة حتى يقوم بإفامه
+
+315
+00:26:30,370 --> 00:26:36,140
+للمستمع هذا الشيء طبيعي لكن لو احتاج الأمرنعيد و
+
+316
+00:26:36,140 --> 00:26:40,920
+نكرر مرة و اثنين و ثلاثة حتى يفهم الحضور ما هو
+
+317
+00:26:40,920 --> 00:26:46,540
+المراد بهذا المعنى فلذلك من هنا لابد التعبير و
+
+318
+00:26:46,540 --> 00:26:50,860
+الحديث و هذه تعتبر مهارة لابد من تدريب القادة على
+
+319
+00:26:50,860 --> 00:26:56,180
+هذا .. على هذا الحوار و هذه اللغة و التعبير الجيد
+
+320
+00:26:56,180 --> 00:27:06,370
+واضح؟ ماذا أيضا؟ انت ماتكلمتيش؟ فضليالمهارة في
+
+321
+00:27:06,370 --> 00:27:10,290
+التسجيل والتقويم وهذا في كل طرق الخدمة الاجتماعية
+
+322
+00:27:10,290 --> 00:27:17,520
+نهتم بالتسجيل ضروري وتقويم أعمالنالابد لماذا نسجل
+
+323
+00:27:17,520 --> 00:27:22,920
+حتى لا ننسى الانسان سمى يا انسان لماذا من النسيان
+
+324
+00:27:22,920 --> 00:27:27,140
+فلذلك إذا يعني أردنا أن لا ننسى لابد أن نقوم
+
+325
+00:27:27,140 --> 00:27:32,420
+بالتسجيل والتسجيل بفيدنا لمعرفة الماضي معرفة
+
+326
+00:27:32,420 --> 00:27:36,580
+الحاضر والمستقبل أيضًا وماذا تم في الماضي إذا
+
+327
+00:27:36,580 --> 00:27:42,260
+الماضي والحاضر وما قمنا بتسجيلهنستفيد منه لخطط
+
+328
+00:27:42,260 --> 00:27:46,600
+مستقبلية في المستقبل، فلذلك التسجيل يفيدنا
+
+329
+00:27:46,600 --> 00:27:52,260
+للمستقبل، للخطط المستقبلية، ماذا نعمل؟ والتقويم
+
+330
+00:27:52,260 --> 00:27:56,080
+أيضًا، في ماذا يفيدنا؟ يفيدنا أيضًا في معرفة
+
+331
+00:27:56,080 --> 00:28:00,460
+الأعمال، ما هي الإيجابيات وما هي السلبيات، وندعم
+
+332
+00:28:00,460 --> 00:28:06,520
+الإيجابيات ونتلاف السلبياتواضح في أعمالنا فلذلك
+
+333
+00:28:06,520 --> 00:28:11,720
+هذه مهارة لابد من تدريبها للقادة والتعود عليها
+
+334
+00:28:11,720 --> 00:28:15,880
+القيام بالتسجيل لأعمالهم باستمرار وفي نفس الوقت
+
+335
+00:28:15,880 --> 00:28:20,120
+القيام بالتقويم لأعمالهم حتى يتعودوا على هذا
+
+336
+00:28:20,120 --> 00:28:25,620
+الإسلوب التسجيل والتقويم هذه كلها بنسميها مهارة
+
+337
+00:28:25,620 --> 00:28:31,120
+هذه كلها مهارات لازمة لغرسها لدى القائد المتدرب
+
+338
+00:28:33,170 --> 00:28:39,950
+طيب في عندنا طرق للتدريب حتى نستطيع أن نقوم بتدريب
+
+339
+00:28:39,950 --> 00:28:44,470
+هؤلاء القادة في تنظيم المجتمع في عندنا طرق معينة
+
+340
+00:28:44,470 --> 00:28:50,870
+طبعا أهم هذه الطرق أول طريقة الدراسة النظرية دراسة
+
+341
+00:28:50,870 --> 00:28:55,690
+محاضرات ندوات إلى غير ذلك هناخدها الآن هنذكرها ثم
+
+342
+00:28:55,690 --> 00:29:01,740
+نشرحكم طريقة من هذه الطرق ثم النوع الثانيالتلمدة
+
+343
+00:29:01,740 --> 00:29:08,340
+النوع الثالث واحدة وخالبة الاشراف الاشراف دراسة
+
+344
+00:29:08,340 --> 00:29:12,560
+الحالات دراسة الحالات تمثيل الأدوار تمثيل الأدوار
+
+345
+00:29:12,560 --> 00:29:16,680
+خلاص خلاص خلاص خلاص خلاص خلاص خلاص خلاص خلاص خلاص
+
+346
+00:29:16,680 --> 00:29:16,680
+خلاص خلاص خلاص خلاص خلاص خلاص خلاص خلاص خلاص خلاص
+
+347
+00:29:16,680 --> 00:29:18,460
+خلاص خلاص خلاص خلاص خلاص خلاص خلاص خلاص خلاص خلاص
+
+348
+00:29:18,460 --> 00:29:18,460
+خلاص خلاص خلاص خلاص خلاص خلاص خلاص خلاص خلاص خلاص
+
+349
+00:29:18,460 --> 00:29:18,460
+خلاص خلاص خلاص خلاص خلاص خلاص خلاص خلاص خلاص خلاص
+
+350
+00:29:18,460 --> 00:29:21,380
+خلاص خلاص خلاص خلاص خلاص خلاص خلاص
+
+351
+00:29:25,160 --> 00:29:27,940
+الدراسة النظرية، إيش يعني الدراسة النظرية؟ مثل
+
+352
+00:29:27,940 --> 00:29:32,720
+ماذا؟ أيوة يا بنت مثل ماذا؟ كلمة عن طريق المحاضرات
+
+353
+00:29:32,720 --> 00:29:36,620
+و كلمة عن زيارات و تمثيل التوضيح والتراحة كويس،
+
+354
+00:29:36,620 --> 00:29:39,080
+يعني الدراسة النظرية زي المحاضرات اللي بتاخدوها
+
+355
+00:29:39,080 --> 00:29:44,020
+أنتوا، فهذه طبعا طريقة من طرق التدريب للقادة،
+
+356
+00:29:44,020 --> 00:29:49,910
+نعطيهم محاضراتحول معلومات معينة أيضا نعطيهم ندوات
+
+357
+00:29:49,910 --> 00:29:55,610
+نعطيهم زيارات ممكن نزور مؤسسات معينة فيها يعني
+
+358
+00:29:55,610 --> 00:30:00,710
+جوانب إدارية يتعرفوا عليها وإنجازات يتعرفوا عليها
+
+359
+00:30:00,710 --> 00:30:04,370
+وكيف تم تحفيق هذه الإنجازات هذه كلها ايش بنسميها
+
+360
+00:30:04,370 --> 00:30:09,870
+زيارات في أيضا تمثيل أحيانا تمثيل التوضيح زي وسائل
+
+361
+00:30:09,870 --> 00:30:16,470
+الإضاح ممكن نوضح نستخدم الأجهزة الحديثةالمعلومات
+
+362
+00:30:16,470 --> 00:30:21,050
+بوسائل الإضاحة المختلفة رسم خريطة مثلا أو رسم شكل
+
+363
+00:30:21,050 --> 00:30:25,770
+معين لتوصيل هذه المعلومة و هكذا نستخدم الأجهزة
+
+364
+00:30:25,770 --> 00:30:29,090
+الحديثة ال LCD و الكمبيوتر و اليوم ما شاء الله
+
+365
+00:30:29,090 --> 00:30:33,350
+أصبح عندنا وسائل حديثة من شاشات العرض هذه كلها
+
+366
+00:30:33,350 --> 00:30:37,270
+يعني وسائل حديثة ممكن نستخدمها في اللي هي القيام
+
+367
+00:30:37,270 --> 00:30:42,580
+بتوصيل المعلوماتللمتدرب هذي اللي بتسميها الدراسة
+
+368
+00:30:42,580 --> 00:30:46,960
+اللي إيه النظرية في عندنا النوع الثاني التلمدة
+
+369
+00:30:46,960 --> 00:30:53,480
+ماذا تعني التلمدة؟ إيش يعني التلمدة؟ خلّي كل طالب
+
+370
+00:30:53,480 --> 00:31:01,140
+يتفكر طريقة من طرق التدريب التلمدة طبعا
+
+371
+00:31:01,140 --> 00:31:07,600
+إحنا بنقوم بتعليم القادة عن طريق التلمدةيعني تلميذ
+
+372
+00:31:07,600 --> 00:31:12,880
+قائد جديد يعتبر إيه؟ تلميذ والقائد القديم يعتبر
+
+373
+00:31:12,880 --> 00:31:17,740
+مداد يعني أه أستاذ هو يعتبر خبير في القيادة إذا
+
+374
+00:31:17,740 --> 00:31:22,940
+هذا القائد الجديد يتعلم من مين؟ من القائد القديم
+
+375
+00:31:22,940 --> 00:31:30,040
+طبعا طبعا زي الطالب و الأستاذ و لذلك بنقول الطالب
+
+376
+00:31:30,040 --> 00:31:35,970
+اللي بنعتبره التلميذواضح و الأستاذ هو القائد فلذلك
+
+377
+00:31:35,970 --> 00:31:41,990
+هنا يعتبر الأستاذ قدوة لتلاميذه أو لطلبته طلاب و
+
+378
+00:31:41,990 --> 00:31:47,290
+طالبات إذا من هنا بنعتبر هذه طريقة من طرق التعلم و
+
+379
+00:31:47,290 --> 00:31:52,380
+التدريب بأن القائد الكبير يكون قدوةليه القادة
+
+380
+00:31:52,380 --> 00:31:56,980
+الصغار؟ فلذلك القادة الصغار يتعلمون من القائد
+
+381
+00:31:56,980 --> 00:32:02,820
+الكبير في ألفاظه، في سلوكه، في تعامله، في المواقف
+
+382
+00:32:02,820 --> 00:32:07,680
+المختلفة، كيف يتصرف في المواقف المختلفة، واضح؟
+
+383
+00:32:07,680 --> 00:32:11,800
+فلذلك هنا يكون قدوة، واضح؟ فلذلك بنقول عنها
+
+384
+00:32:11,800 --> 00:32:16,620
+التلمدة، وهو تتلمد القادة الصغار على أيدي القادة
+
+385
+00:32:16,620 --> 00:32:20,880
+الكبارأصلا مفهومة بهذه الشكل؟ خلاص تفهمنا الطريقة
+
+386
+00:32:20,880 --> 00:32:26,300
+هذه؟ من السهل جدا يعني نفهم، خلاص نيجي الآن
+
+387
+00:32:26,300 --> 00:32:34,440
+للإشراف وهذه طريقة من طرق التدريب أيضا الإشراف،
+
+388
+00:32:34,440 --> 00:32:40,060
+مين تقولي إيش يعني الإشرافة؟ لما اتكلمتش نهائيا،
+
+389
+00:32:40,060 --> 00:32:45,300
+ماذا تعني طريقة الإشراف في التدريب؟ أيوة بنتياللي
+
+390
+00:32:45,300 --> 00:32:48,460
+ماتكلمتش احكي انا؟ احكي اتفضلي ماعرف ان هو واحد
+
+391
+00:32:48,460 --> 00:32:53,900
+النقص و الامتياز في العددأه .. أيها ابنتي نعم أن
+
+392
+00:32:53,900 --> 00:32:57,120
+يكون فيه قائد أكبر و هم عدة قادة و القائد الأكبر
+
+393
+00:32:57,120 --> 00:33:00,320
+هو المشرف عليهم .. هم يعني .. هو المارس أعملهم ..
+
+394
+00:33:00,320 --> 00:33:03,880
+هو بيعرف .. بس يعرفهم جوانب النقص .. و الإيجابية
+
+395
+00:33:03,880 --> 00:33:05,820
+السلبية المناعمة .. توجيه .. بالظبط .. بالظبط ..
+
+396
+00:33:05,820 --> 00:33:10,220
+الإشراف يعني التوجيه و هذا بن .. بنقول عنها في
+
+397
+00:33:10,220 --> 00:33:14,160
+التدريب الميداني عندنا لما ناخد طلبتنا و ندربهم
+
+398
+00:33:14,160 --> 00:33:19,240
+ميدانيافهنا يعني بيكون معهم مشرف في المؤسسة ومشرف
+
+399
+00:33:19,240 --> 00:33:24,280
+من الجامعة وهنا يذلل لهم الصعاب ويوجههم في القيام
+
+400
+00:33:24,280 --> 00:33:29,360
+في اللي هي عملية التوجيه المعني للتدريب واكتساب
+
+401
+00:33:29,360 --> 00:33:33,740
+الخبرات من المؤسسة التي يتدربون فيها فلذلك الإشراف
+
+402
+00:33:33,740 --> 00:33:38,900
+في القيادة هنا لابد أن يكون المشرفموجهًا لهؤلاء
+
+403
+00:33:38,900 --> 00:33:44,460
+المتدربين أن يقوم بتعليمهم وتدريبهم وتوجيههم، يعني
+
+404
+00:33:44,460 --> 00:33:48,000
+توجيههم التوجيه الصحيح والسليم أثناء قيامهم
+
+405
+00:33:48,000 --> 00:33:53,200
+الأعمال الإدارية أو القيادية داخل المجتمع ويصحح
+
+406
+00:33:53,200 --> 00:33:56,880
+لهم الأخطاء ويعدلها، فهذا ما نقول عنه إشراف
+
+407
+00:33:56,880 --> 00:34:03,460
+وتوجيه، واضح؟هذه بالنسبة لطريقة من طرق أيضا اللى
+
+408
+00:34:03,460 --> 00:34:10,140
+هو التدريب اللى هو الإشراف والتوجيه ماذا أيضا من
+
+409
+00:34:10,140 --> 00:34:18,020
+طرق التدريب دراسة الحالات دراسة الحالات نعم دراسة
+
+410
+00:34:18,020 --> 00:34:24,620
+الحالات ماشي
+
+411
+00:34:24,620 --> 00:34:28,920
+الحال دراسة الحالات إيش يعني دراسة الحالات؟
+
+412
+00:34:33,380 --> 00:34:37,220
+هي عرض المدرّب حالة من البرنامج برنامج نوبة
+
+413
+00:34:37,220 --> 00:34:41,180
+بالمجتمع لمناخسة الجماعة من حيث الإجراءات والفتوات
+
+414
+00:34:41,180 --> 00:34:45,800
+التي تم بها لذلك لدى تعيش الجماعة في جو حقيقي
+
+415
+00:34:45,800 --> 00:34:50,900
+بالضبط يعني دراسة تطبيقية لحالة معينة وتعليمة
+
+416
+00:34:50,900 --> 00:34:55,220
+أولاء القادة على كيفية التصرف مع هذه الحالة مثل
+
+417
+00:34:55,220 --> 00:34:59,100
+دراسة الحالات الفردية والجماعية والمجتمعية الاخرى
+
+418
+00:34:59,100 --> 00:35:03,560
+في تمثيل الأدوار تعتمد على ماذا؟نعم يا بنتى
+
+419
+00:35:08,070 --> 00:35:12,330
+بالضبط ممكن نخرجه عن طريق تمثيلية أو مسرحية للقيام
+
+420
+00:35:12,330 --> 00:35:15,990
+بهذه الأدوار وهنا عندما يقوم الإخراج بتمثيل
+
+421
+00:35:15,990 --> 00:35:21,390
+الأدوار ممكن يكون لها تأثير أقوى لدى القادة عند
+
+422
+00:35:21,390 --> 00:35:26,310
+مشاهدة هذه التمثيلية بالقيام بدور القائد في هذه
+
+423
+00:35:26,310 --> 00:35:30,030
+المواقف المختلفة فهذه طريقة أيضا من طراب تدريب
+
+424
+00:35:30,030 --> 00:35:36,630
+القادة وهي تمثيل الأدوار المختلفةواضح؟ يعني بذلك
+
+425
+00:35:36,630 --> 00:35:41,050
+نكون قد انتهينا من طرق التدريب واختيار القادة
+
+426
+00:35:41,050 --> 00:35:46,610
+وكيفية تدريبهم وايضا كيفية اختيارهم وخاصة القادة
+
+427
+00:35:46,610 --> 00:35:51,830
+الشعبيين وطرق التدريب اللازمة لهم حتى نستطيع أن
+
+428
+00:35:51,830 --> 00:35:56,600
+نتعامل مع هؤلاء القادةاللي هم المنظم الاجتماعي و
+
+429
+00:35:56,600 --> 00:36:00,720
+القادة لكي يتعاملوا مع بعض من بعض من أجل مصلحة
+
+430
+00:36:00,720 --> 00:36:05,320
+المجتمع و مساعدة المجتمع على فهم مشكلته و كيفية
+
+431
+00:36:05,320 --> 00:36:09,500
+معالجة المشكلات المختلفة ان شاء الله في المحاضرة
+
+432
+00:36:09,500 --> 00:36:17,500
+القادمة سنتناول موضوع التطوعالمشاركة التطوعية في
+
+433
+00:36:17,500 --> 00:36:23,580
+تنظيم المجتمع فإن شاء الله بإذن الله سنشرح أو نبدأ
+
+434
+00:36:23,580 --> 00:36:26,940
+في شرح هذا الموضوع في المحاضرة القادمة إلى أن
+
+435
+00:36:26,940 --> 00:36:36,420
+نلتقي بكنا نتمنى لكنا وقتا طيبا وإلى أن نلتقي لكنا
+
+436
+00:36:36,420 --> 00:36:41,200
+من كل تحيط وتقدير والسلام عليكم ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwZ8bh257eMcqZ22bPBOhO8z/iICaAA1ruDo.srt b/PL9fwy3NUQKwZ8bh257eMcqZ22bPBOhO8z/iICaAA1ruDo.srt
new file mode 100644
index 0000000000000000000000000000000000000000..d8dc1de21d632e0b84091b5546fac7aa57b33e46
--- /dev/null
+++ b/PL9fwy3NUQKwZ8bh257eMcqZ22bPBOhO8z/iICaAA1ruDo.srt
@@ -0,0 +1,1078 @@
+1
+00:00:21,010 --> 00:00:25,370
+بسم الله الرحمن الرحيم الحمد لله رب العالمين
+
+2
+00:00:25,370 --> 00:00:30,050
+والصلاة والسلام على أشرف المرسلين سيدنا محمد وعلى
+
+3
+00:00:30,050 --> 00:00:35,470
+آله وصحبه أجمعين، بنات الطالبات، السلام عليكم ورحمة
+
+4
+00:00:35,470 --> 00:00:41,370
+الله وبركاته. في المحاضرة السابقة كنا نتحدث عن دور
+
+5
+00:00:41,370 --> 00:00:47,970
+المنظم الاجتماعي، وتحدثنا عن مفهوم الدور ومكونات
+
+6
+00:00:47,970 --> 00:00:54,140
+الدور ونظرية الدور. في هذه المحاضرة سنتعرف على
+
+7
+00:00:54,140 --> 00:00:59,360
+العوامل التي أدت لاختلاف الكتاب عند صياغتهم لأدوار
+
+8
+00:00:59,360 --> 00:01:05,240
+المنظم الاجتماعي، وطبعًا في عدد من العوامل سنناقش
+
+9
+00:01:05,240 --> 00:01:09,860
+هذه. سنذكر هذه العوامل ونناقش كل عامل على حدة.
+
+10
+00:01:10,520 --> 00:01:14,760
+طبعًا من هذه العوامل التي أدت إلى اختلاف الكتاب عند
+
+11
+00:01:14,760 --> 00:01:20,360
+كتابتهم وصياغتهم لدور المنظم الاجتماعي، أول شيء
+
+12
+00:01:20,360 --> 00:01:25,600
+اختلاف مستوى التجريد، ثم اختلاف النماذج، ثم اختلاف
+
+13
+00:01:25,600 --> 00:01:30,020
+المراحل التاريخية، ثم بعد ذلك اختلاف مجالات العمل،
+
+14
+00:01:30,020 --> 00:01:35,740
+ثم اختلاف الظروف والتيارات الاجتماعية السائدة. هذه
+
+15
+00:01:35,740 --> 00:01:41,830
+النقاط كلها سنناقشها نقطة نقطة، لكي نتعرف على
+
+16
+00:01:41,830 --> 00:01:46,530
+الأسباب التي أدت إلى اختلاف الكتاب حول صياغتهم
+
+17
+00:01:46,530 --> 00:01:52,130
+لدور المنظم الاجتماعي. سنأخذ النقطة الأولى، وهي
+
+18
+00:01:52,130 --> 00:01:57,310
+اختلاف مستوى التجريد. طبعًا، ماذا يعني اختلاف مستوى
+
+19
+00:01:57,310 --> 00:02:01,740
+التجريد؟ نعم يا بنتي، أفضل بعض الكتاب القيام حسب ما
+
+20
+00:02:01,740 --> 00:02:05,720
+يقوم به معظم من خلال عمله اليومي في مجال أو مجالات
+
+21
+00:02:05,720 --> 00:02:10,180
+متقاربة، من خلال تصنيف أعمالهم تحت بعض العناوين
+
+22
+00:02:10,180 --> 00:02:14,580
+العامة، ثم التوصل إلى دور معظم، ليعرف دور الوصفة
+
+23
+00:02:14,610 --> 00:02:17,990
+بالضبط. يعني من خلال عمله، من خلال عمل المنظم في
+
+24
+00:02:17,990 --> 00:02:22,390
+المؤسسات الاجتماعية، يحصر دوره، وخاصة المؤسسات
+
+25
+00:02:22,390 --> 00:02:26,670
+المتشابهة في المجال وفي ميدان العمل الواحد، في
+
+26
+00:02:26,670 --> 00:02:29,930
+مجال الخدمة الاجتماعية الواحد أو ميدان العمل
+
+27
+00:02:29,930 --> 00:02:34,310
+الواحد للخدمة الاجتماعية، فيقوم المنظم بحصر الأدوار
+
+28
+00:02:34,310 --> 00:02:37,430
+التي يقوم بها في هذا المجال، من خلال عدد من
+
+29
+00:02:37,430 --> 00:02:40,870
+المؤسسات التي يعملون فيها، وهم الأخصائيين
+
+30
+00:02:40,870 --> 00:02:45,150
+الاجتماعيين. فكل واحد يكتب  أيّ وصف، الدور الوصفي
+
+31
+00:02:45,150 --> 00:02:50,310
+لما يقوم به داخل المؤسسة من أعمال في مجال واحد،
+
+32
+00:02:50,310 --> 00:02:55,730
+ومن مجموع هذه الكتابات للدور الوصفي، ممكن نصل إلى
+
+33
+00:02:55,730 --> 00:03:01,010
+كتابة معينة أو إلى وصف معين لدور المنظم الاجتماعي،
+
+34
+00:03:01,010 --> 00:03:05,650
+من خلال الصياغة الوصفية لدور المنظم الاجتماعي في
+
+35
+00:03:05,650 --> 00:03:11,130
+مجالات العمل المتشابهة، عندما يقوم بها في ميدان طريق
+
+36
+00:03:11,130 --> 00:03:15,810
+التنظيم المجتمعي، أو هكذا. فلذلك من هنا يحصر الدور
+
+37
+00:03:15,810 --> 00:03:19,470
+الوصفي لدور المنظم الاجتماعي عن طريق حصر كل ما
+
+38
+00:03:19,470 --> 00:03:23,750
+يقوم به من أعمال. ثم بعد ذلك قد يعني يجعل من هذا
+
+39
+00:03:23,750 --> 00:03:29,690
+الوصف، الذي هو الدور الكامل، والدور يعني نسميه قد
+
+40
+00:03:29,690 --> 00:03:34,130
+يكون الدور الذي له طابع تجريدي، الذي يقوم بوصفه
+
+41
+00:03:34,130 --> 00:03:39,280
+المنظم الاجتماعي. وفي هذه الحالة يعني يوضع من ضمن
+
+42
+00:03:39,280 --> 00:03:44,240
+الدور، المنظم الاجتماعي في طريق التنظيم المجتمعي في
+
+43
+00:03:44,240 --> 00:03:48,140
+مجال معين من مجالات طريق التنظيم المجتمعي.
+
+44
+00:03:56,270 --> 00:03:59,890
+طيب، خلاص فهمنا النقطة الأولى. نأتي إلى النقطة
+
+45
+00:03:59,890 --> 00:04:03,970
+الثانية، اختلاف النماذج. أيوة يا بنتي، بسبب صعوبة
+
+46
+00:04:03,970 --> 00:04:07,750
+عملية التجريد، اعتمد أكثر المنظمين، قرروا أنهم
+
+47
+00:04:07,750 --> 00:04:10,950
+يعتمدوا نموذجًا واحدًا، عشان هم يقدروا يعملوا عملية
+
+48
+00:04:10,950 --> 00:04:15,050
+التجريد. فكل واحد من المنظمين اعتمد نموذجًا، الذي هو
+
+49
+00:04:15,050 --> 00:04:18,750
+يعني يعتقد أنه هو الأفضل. فمنهم اعتمد نظرية خدمة
+
+50
+00:04:18,750 --> 00:04:21,970
+الفرد، أو نموذج خدمة الفرد، ومنهم اعتمد نموذج خدمة
+
+51
+00:04:21,970 --> 00:04:25,960
+الجماعة، وهكذا اختلفوا في... نعم، اختلفوا في الكتابات،
+
+52
+00:04:25,960 --> 00:04:30,740
+نعم، إذن من... التي هي اختلافهم في، التي هي عمل
+
+53
+00:04:30,740 --> 00:04:34,940
+النماذج، أو كتابة النماذج، التي هي... ولذلك اختلفوا
+
+54
+00:04:34,940 --> 00:04:38,940
+هنا في النموذج الذي اتخذوه في عملية التجريد. فهذا
+
+55
+00:04:38,940 --> 00:04:43,480
+يُعتبر برضه سببًا من أسباب اختلافهم في صياغة دور
+
+56
+00:04:43,480 --> 00:04:47,560
+المنظم الاجتماعي. إذن السبب الأول هو اختلافهم من حيث
+
+57
+00:04:47,560 --> 00:04:53,000
+التجريد، السبب الثاني هو من حيث النماذج. طيب،
+
+58
+00:04:53,810 --> 00:04:57,450
+طبعًا بعض الكتاب يُفضل القياس أحيانًا على نموذج
+
+59
+00:04:57,450 --> 00:05:03,270
+أخصائي خدمة الفرد، أو أخصائي، أو مثلًا أحيانًا يأخذ
+
+60
+00:05:03,270 --> 00:05:07,310
+زي نموذج دور روس، والبعض الآخر طبعًا نموذج دور
+
+61
+00:05:07,310 --> 00:05:11,290
+روس، التي هي التنمية، دور التنموي في طريق تنظيم
+
+62
+00:05:11,290 --> 00:05:16,090
+المجتمع، لأنه ربط طريق تنظيم المجتمع بالتنمية أيضًا.
+
+63
+00:05:16,090 --> 00:05:20,290
+في نموذج آخر، خدمة الجماعة، مثل ما سنأخذ دور الـ New
+
+64
+00:05:20,290 --> 00:05:26,160
+Staters، الذي ربطه بخدمة الجماعة، وهكذا. فلذلك من هنا
+
+65
+00:05:26,160 --> 00:05:31,660
+اختلفت، التي هي النماذج، عند صياغة دور، أو اختلفت،
+
+66
+00:05:31,660 --> 00:05:35,960
+اختلف الكتاب في صياغة دور المنظم الاجتماعي عن طريق
+
+67
+00:05:35,960 --> 00:05:40,340
+اختلافهم في التي هي النماذج المختلفة، سواء كان
+
+68
+00:05:40,340 --> 00:05:44,720
+نموذج خدمة الفرد، أو نموذج خدمة الجماعة، أو حتى في
+
+69
+00:05:44,720 --> 00:05:48,800
+نماذج في طريقة تنظيم المجتمع، عند ربط طريقة تنظيم
+
+70
+00:05:48,800 --> 00:05:53,690
+المجتمع بالتنمية الاجتماعية، مثل دور روس. في عندنا
+
+71
+00:05:53,690 --> 00:05:59,750
+الآن سبب آخر أيضًا لاختلاف الكتاب أو العلماء لكتاب
+
+72
+00:05:59,750 --> 00:06:04,230
+دور محدد لدور المنظم الاجتماعي، ما هو هذا السبب
+
+73
+00:06:04,230 --> 00:06:10,810
+الثالث؟ سبب
+
+74
+00:06:10,810 --> 00:06:16,650
+ثالث، سبب واضح، التي هي المراحل التاريخية التي ظهرت
+
+75
+00:06:16,650 --> 00:06:18,090
+فيها طريقة تنظيم المجتمع.
+
+76
+00:06:20,680 --> 00:06:25,520
+وكل مرحلة من هذه المراحل أدت إلى صياغة دور. أنتم
+
+77
+00:06:25,520 --> 00:06:28,660
+ما عرفتم مراحل طريقة تنظيم المجتمع، مش عندنا أربع
+
+78
+00:06:28,660 --> 00:06:34,500
+مراحل: مرحلة التنسيقية أولًا، ثم المرحلة التخطيطية، ثم
+
+79
+00:06:34,500 --> 00:06:40,580
+مرحلة التنمية، ثم مرحلة الدفاع. في كل مرحلة من هذه
+
+80
+00:06:40,580 --> 00:06:46,480
+المراحل كان هناك دور للمنظم الاجتماعي، ولذلك اختلف
+
+81
+00:06:46,480 --> 00:06:51,160
+الكتاب في صياغة الدور حسب المرحلة التاريخية لتطور
+
+82
+00:06:51,160 --> 00:06:57,450
+طريق تنظيم المجتمع. واضح، فلذلك من هنا يعني في أدوار
+
+83
+00:06:57,450 --> 00:07:01,290
+مختلفة. لأن أنتم عارفين إحنا طريق التنظيم المجتمعي ظهر
+
+84
+00:07:01,290 --> 00:07:07,610
+عام 1946، ظهر بالمرحلة التنسيقية، ثم التخطيطية، ثم
+
+85
+00:07:07,610 --> 00:07:12,110
+التنمية، ثم الدفاع. في كل مرحلة من هذه المراحل ظهر
+
+86
+00:07:12,110 --> 00:07:16,910
+دور للمنظم الاجتماعي: دور في مرحلة التنسيق، دور في
+
+87
+00:07:16,910 --> 00:07:21,450
+مرحلة التخطيط كمخطط، دور في مرحلة التنمية كدور الـ
+
+88
+00:07:21,450 --> 00:07:26,010
+... الـ... الممكن والمنمي، الذي هو في التنمية، ودور
+
+89
+00:07:26,010 --> 00:07:30,030
+المدافع، وهكذا. إذن من هنا اختلف الكتاب حسب
+
+90
+00:07:30,030 --> 00:07:34,710
+المراحل التاريخية في كتابتهم لدور المنظم الاجتماعي.
+
+91
+00:07:34,710 --> 00:07:41,910
+طيب، في عندنا أيضًا... هذه كلها يعني التي نحن ذكرناها،
+
+92
+00:07:41,910 --> 00:07:45,250
+الدور المنسق، الدور المخطط، الدور المنمي، الدور
+
+93
+00:07:45,250 --> 00:07:50,710
+المدافع أو الثوري، التي هي آخر الأدوار، وهي آخر
+
+94
+00:07:50,710 --> 00:07:56,570
+مرحلة من مراحل تطور طريق تنظيم المجتمع. في عندنا
+
+95
+00:07:56,570 --> 00:08:01,670
+سبب آخر أيضًا من الأسباب التي أدت إلى اختلاف الكتاب
+
+96
+00:08:01,670 --> 00:08:05,770
+عند صياغتهم لدور المنظم... نعم يا بنتي، في أغلب
+
+97
+00:08:05,770 --> 00:08:10,070
+الكتاب يختلفون في صياغتهم لهذا النموذج في نوع معين
+
+98
+00:08:10,070 --> 00:08:13,790
+من  الأجهزة أو مستوى من المستويات. فروت... روث تأثر
+
+99
+00:08:13,790 --> 00:08:18,650
+بكتابات تنمية المشاريع، المحل الصغير، أما روبرت و...
+
+100
+00:08:18,650 --> 00:08:21,710
+والـnewsletter تأثر بمجالات... مجالس الهيئات
+
+101
+00:08:21,710 --> 00:08:25,290
+الاجتماعية، أما كتابات البروفسير وغيرهم من الكتاب
+
+102
+00:08:25,290 --> 00:08:31,240
+تأثروا بقدر... إذن هذا سبب أيضًا من أسباب اختلاف
+
+103
+00:08:31,240 --> 00:08:35,680
+الكتاب عند صياغتهم لدور المنظم الاجتماعي، وهو
+
+104
+00:08:35,680 --> 00:08:40,700
+اختلاف مجالات العمل. فكل كاتب أو كل عالم من علماء
+
+105
+00:08:40,700 --> 00:08:44,920
+طريق التنظيم المجتمعي صاغ دور المنظم حسب المجال
+
+106
+00:08:44,920 --> 00:08:50,020
+الذي يعمل فيه أو المجال الذي يكتب من خلاله. مثل دور
+
+107
+00:08:50,020 --> 00:08:56,280
+روس، يعني صياغته لدور المنظم، التي هي ربطه بالتنمية
+
+108
+00:08:56,280 --> 00:09:00,970
+الاجتماعية. ربط طريقة دور المنظم الاجتماعي في طريقة
+
+109
+00:09:00,970 --> 00:09:05,750
+تنظيم المجتمع، ربطه بدور التنمية الاجتماعية، والـ
+
+110
+00:09:05,750 --> 00:09:08,590
+New Staters، ربطه بدور... ربط دور ماذا؟ الـ New
+
+111
+00:09:08,590 --> 00:09:11,890
+Staters بالتنسيق، التي هي التنسيق بين الجامعات،
+
+112
+00:09:11,890 --> 00:09:16,490
+التنسيق بين الجامعات ومجالس الهيئات الاجتماعية
+
+113
+00:09:16,490 --> 00:09:23,570
+المختلفة. أيضًا دور Grosser، ربطه أيضًا بالحقوق
+
+114
+00:09:23,570 --> 00:09:29,550
+المدنية والعمل مع الفقراء، وهكذا. إذن كل كاتب ربط
+
+115
+00:09:29,550 --> 00:09:35,290
+دور المنظم حسب... حسب ما كان يعني يهوى ويعمل فيه
+
+116
+00:09:35,290 --> 00:09:38,750
+في مجال العمل الذي كان يعمل فيه، فربط دور المنظم
+
+117
+00:09:38,750 --> 00:09:46,160
+بمجال العمل الذي يعمل فيه ويكتب فيه. في عندنا
+
+118
+00:09:46,160 --> 00:09:50,900
+اختلاف الظروف والتيارات الاجتماعية. كذلك هذا سبب من
+
+119
+00:09:50,900 --> 00:09:56,140
+أسباب اختلاف الكتاب حول كتابتهم لدور المنظم
+
+120
+00:09:56,140 --> 00:10:00,800
+الاجتماعي والوصول إلى دور واحد. فلذلك من هنا
+
+121
+00:10:00,800 --> 00:10:05,220
+اختلفوا حول الأدوار. فهذا اختلاف الظروف والتيارات
+
+122
+00:10:05,220 --> 00:10:08,620
+الاجتماعية السائدة، طبعًا.
+
+123
+00:10:10,110 --> 00:10:14,870
+في أحيانًا تيارات ديمقراطية، تيارات دكتاتورية،
+
+124
+00:10:14,870 --> 00:10:20,770
+تيارات رأسمالية، تيارات اشتراكية، واضح. أيضًا إحنا
+
+125
+00:10:20,770 --> 00:10:25,170
+في مجتمعنا العربي كذلك في تيارات إسلامية، قومية،
+
+126
+00:10:25,170 --> 00:10:31,420
+وهكذا. إذن التيارات هذه لها كذلك تأثير عند صياغة دور
+
+127
+00:10:31,420 --> 00:10:35,940
+المنظم الاجتماعي في المجتمع. فلذلك لابد أن نلمّ بهذه
+
+128
+00:10:35,940 --> 00:10:40,400
+التيارات المختلفة عند دراستنا لصياغة دور المنظم
+
+129
+00:10:40,400 --> 00:10:43,600
+الاجتماعي، ونعرف دور المنظم الاجتماعي في المجتمع
+
+130
+00:10:43,600 --> 00:10:47,300
+الاشتراكي ما هو، ودور المنظم الاجتماعي في المجتمع
+
+131
+00:10:47,300 --> 00:10:51,490
+الرأسمالي ما هو، مجتمع الغرب الرأسمالي، ونعرف دور
+
+132
+00:10:51,490 --> 00:10:55,250
+المنظم الاجتماعي في مجتمعنا العربي الإسلامي كذلك
+
+133
+00:10:55,250 --> 00:11:00,170
+ما هو. فلذلك من هنا الكتاب يختلفون في صياغتهم لدور
+
+134
+00:11:00,170 --> 00:11:06,970
+المنظم حسب التيارات السياسية والعقائدية، وأيضًا كل
+
+135
+00:11:06,970 --> 00:11:11,030
+هذه التيارات يعني كانت سببًا في اختلافهم عند
+
+136
+00:11:11,030 --> 00:11:16,010
+صياغتهم لدور المنظم الاجتماعي. طيب،
+
+137
+00:11:18,360 --> 00:11:23,220
+في عندنا أيضًا، يعني مثلًا دور المنظم الاجتماعي،
+
+138
+00:11:23,220 --> 00:11:28,500
+يعني هذا كان في مرحلة أخيرة من مراحل تطور طريق
+
+139
+00:11:28,500 --> 00:11:32,980
+تنظيم المجتمع، عند قيام الذين هم ثورة الفقراء
+
+140
+00:11:32,980 --> 00:11:37,200
+والزنوج في أمريكا. فلذلك ظهر دور المنظم الاجتماعي
+
+141
+00:11:37,200 --> 00:11:41,360
+في السبعينيات، وفي حرب فيتنام، عندما صار الشباب على
+
+142
+00:11:41,360 --> 00:11:45,960
+حكومتهم من أجل الاعتراض على الحرب الفيتنامية، الحرب
+
+143
+00:11:45,960 --> 00:11:50,540
+بين أمريكا وفيتنام، فثار الشباب، فأصبح هناك ظهر دور
+
+144
+00:11:50,540 --> 00:11:54,480
+المدافع في أمريكا، دور المدافع في طريق تنظيم
+
+145
+00:11:54,480 --> 00:11:58,460
+المجتمع وفي الخدمة الاجتماعية. ولذلك الزنوج كذلك
+
+146
+00:11:58,460 --> 00:12:04,080
+ثورة الزنوج ضد البيض، هذا جعل أن يظهر هناك دور جديد
+
+147
+00:12:04,080 --> 00:12:08,280
+لطريق تنظيم المجتمع، وهو دور المدافع عن حقوق هؤلاء
+
+148
+00:12:08,280 --> 00:12:12,920
+المظلومين، الزنوج من البيض، طبعًا؛ لأن الزنوج هم أصل
+
+149
+00:12:12,920 --> 00:12:17,340
+البلاد في أمريكا، أصل البلاد في أمريكا هم الزنوج، مثل
+
+150
+00:12:17,340 --> 00:12:21,520
+الذي نحن في فلسطين، أصل الفلسطينيين في فلسطين هم
+
+151
+00:12:21,520 --> 00:12:26,500
+الفلسطينيون. فلذلك من هنا هناك قد يكون صراع ما بين
+
+152
+00:12:26,500 --> 00:12:30,800
+الحق والباطل، ما بين الفئات الضعيفة والفئات القوية
+
+153
+00:12:30,800 --> 00:12:34,280
+المستبدة. فلذلك من هنا ظهرت الأدوار في الخدمة
+
+154
+00:12:34,280 --> 00:12:38,580
+الاجتماعية، التي هي دور المدافع، كذلك الفقراء
+
+155
+00:12:39,030 --> 00:12:43,890
+ومطالباتهم بحقوقهم من الأغنياء وغير ذلك. هذا كله
+
+156
+00:12:43,890 --> 00:12:49,550
+جعل لدور المنظم الاجتماعي كدور مدافع في آخر مراحل
+
+157
+00:12:49,550 --> 00:12:54,130
+تطور طريق تنظيم المجتمع، يظهر بوضوح منذ السبعينيات
+
+158
+00:12:54,130 --> 00:12:58,550
+في أمريكا، وبعد ذلك انتشر هذا في العالم الغربي
+
+159
+00:12:58,550 --> 00:13:02,010
+والعالم العربي كذلك، من خلال النقابات المهنية
+
+160
+00:13:02,010 --> 00:13:06,790
+والجمعيات المطالبة بحقوق الإنسان، والنقابات التي
+
+161
+00:13:06,790 --> 00:13:12,170
+تطالب بحقوق العاملين فيها. هذا كله يعني يركز لنا على
+
+162
+00:13:12,170 --> 00:13:17,250
+دور الـ... المدافع، كدور للمنظم الاجتماعي، الذي هو
+
+163
+00:13:17,250 --> 00:13:21,450
+دور المدافع في طريق تنظيم المجتمع وفي الخدمة
+
+164
+00:13:21,450 --> 00:13:23,170
+الاجتماعية كمهنة.
+
+165
+00:13:27,350 --> 00:13:33,670
+في عندنا يعني نستعرض الآراء الآن، يعني ممكن إحنا
+
+166
+00:13:33,670 --> 00:13:38,470
+عرفنا العوامل التي أدت لاختلاف الكتاب، أو اختلاف
+
+167
+00:13:38,470 --> 00:13:43,790
+العلماء، أو الاختلاف، يعني المختصين في تنظيم المجتمع،
+
+168
+00:13:43,790 --> 00:13:47,350
+عند صياغتهم لدور المنظمة. الآن بدنا نأخذ وجهة نظر
+
+169
+00:13:47,350 --> 00:13:52,430
+الغرب، ووجهة نظر العرب، يعني الكتاب العرب، العلماء في
+
+170
+00:13:52,430 --> 00:13:58,710
+دور المنظم الاجتماعي. فسناخذ بعض العلماء الغرب عند
+
+171
+00:13:58,710 --> 00:14:03,670
+صياغتهم لدور المنظم الاجتماعي، وبعد ذلك إن شاء الله
+
+172
+00:14:03,670 --> 00:14:10,630
+سنتناول دور الذين هم العلماء والمختصين من أساتذتنا،
+
+173
+00:14:10,630 --> 00:14:14,370
+رحم الله عليهم من توفاه الله، وأيضًا رحمة الله على
+
+174
+00:14:14,370 --> 00:14:18,870
+من هو موجود. فالرحمة تجوز على من توفاه الله وعلى من
+
+175
+00:14:
+
+223
+00:18:17,840 --> 00:18:24,020
+المجتمع. فإذا تأثر هنا، اللي هو أكد على دور المنظم
+
+224
+00:18:24,020 --> 00:18:29,240
+اللي هو أول شيء، اللي هو العمل بين الجماعات، وأيضا
+
+225
+00:18:29,240 --> 00:18:33,840
+ركز على اللي هم كـممثلي للوحدات الاجتماعية، اللي هي
+
+226
+00:18:33,840 --> 00:18:38,100
+بين منظمات الجماعات، منظمات الجماعات المختلفة، اللي
+
+227
+00:18:38,100 --> 00:18:43,530
+بتعمل في مجال خدمة الجماعة. في عندنا أيضًا دور
+
+228
+00:18:43,530 --> 00:18:46,370
+المنظم الاجتماعي، من وجهة نظر الـNeustatter، اللي هي
+
+229
+00:18:46,370 --> 00:18:50,970
+العمليات الإدارية. ركزنا أيضًا على العمليات
+
+230
+00:18:50,970 --> 00:18:54,790
+الإدارية، الدور اللي بقوم به اللي هو المنظم
+
+231
+00:18:54,790 --> 00:18:58,670
+الاجتماعي، بعد العمل بين الجماعات، اللي هي العمليات
+
+232
+00:18:58,670 --> 00:18:59,890
+الإدارية.
+
+233
+00:19:01,780 --> 00:19:06,620
+العمليات الإدارية، ووظائف الإدارة، وغير ذلك. فلذلك من
+
+234
+00:19:06,620 --> 00:19:10,080
+هنا لابد من إعداد البناء الملائم اللي بتنفذه
+
+235
+00:19:10,080 --> 00:19:13,400
+العمليات المهنية بين الجماعات، والتأكد من وجود
+
+236
+00:19:13,400 --> 00:19:17,300
+آليات الأداء الملائمة، ووجود الإعداد الكافي من
+
+237
+00:19:17,300 --> 00:19:21,400
+الموظفين، وتوزيع المسؤوليات، وتنظيم الإشراف على
+
+238
+00:19:21,400 --> 00:19:25,600
+النواحي المالية، وتنظيم العلاقات العامة. كل ذلك ما
+
+239
+00:19:25,600 --> 00:19:30,780
+يتعلق بوظائف الإدارة. وظائف الإدارة بتدرسوها إدارة
+
+240
+00:19:30,780 --> 00:19:36,000
+ولا لأ؟ تمشوا أنتو اللي بتدرسوا في الإدارة. احنا
+
+241
+00:19:36,000 --> 00:19:39,840
+بندرّسها، تعاون سنة رابعة. سنة رابعة احنا بندرسها
+
+242
+00:19:39,840 --> 00:19:43,140
+إدارة. فهذه كل اللي ذكرناها من وظائف الإدارة،
+
+243
+00:19:43,140 --> 00:19:50,080
+التنسيق، والإشراف، والتخطيط، والتوجيه، والتمويل. كل هذه
+
+244
+00:19:50,080 --> 00:19:54,720
+من وظائف الإدارة، توزيع المسؤوليات، والأدوار. كل هذه
+
+245
+00:19:54,720 --> 00:19:59,050
+من وظائف الإدارة. فلذلك من هنا ركّزنا على العمليات
+
+246
+00:19:59,050 --> 00:20:03,170
+الإدارية اللي ممكن يجيبها المنظم الاجتماعي، يعني
+
+247
+00:20:03,170 --> 00:20:07,930
+من خلال عملهم في المؤسسات الاجتماعية المختلفة في
+
+248
+00:20:07,930 --> 00:20:13,930
+المجتمع. واضح؟ وأيضا التأكد أيضا من تشكيل اللجان
+
+249
+00:20:13,930 --> 00:20:19,090
+كأدوات من أدوات طريق التنظيم المجتمعي، وأيضا تعتبر
+
+250
+00:20:19,090 --> 00:20:24,110
+بناء من البناء التكويني للمنظمة الاجتماعية. إن
+
+251
+00:20:24,110 --> 00:20:29,390
+المنظمة الاجتماعية لها ثلاث مكونات: أولا، الجمعية
+
+252
+00:20:29,390 --> 00:20:35,150
+العمومية، ثم مجلس الإدارة، ثم اللجان. واللجان هنا هي
+
+253
+00:20:35,150 --> 00:20:42,690
+الأداة الفاعلة من أجل تحقيق أهداف المؤسسة. واضح؟ طيب
+
+254
+00:20:42,690 --> 00:20:48,890
+في عندنا أيضًا، بالإضافة للعمل بين الجماعات، و
+
+255
+00:20:48,890 --> 00:20:52,630
+القيام بالعمليات الإدارية، أيضًا العمليات التربوية.
+
+256
+00:20:52,630 --> 00:20:57,590
+العمليات التربوية. فرّكزنا على، من وجهة نظر، و
+
+257
+00:20:57,590 --> 00:21:01,570
+Newsletter، أيضًا على العمليات التربوية، وهذه تتضمن
+
+258
+00:21:01,570 --> 00:21:07,270
+التدريب، والتثقيف، والتوعية. واضح؟ سواءً للعمال داخل
+
+259
+00:21:07,270 --> 00:21:10,890
+المؤسسة، اللي هو جهاز تنظيم المجتمع اللي بنسميه
+
+260
+00:21:10,890 --> 00:21:15,250
+الهيئة الاجتماعية، أو الجهاز، أو في المجتمع ككل
+
+261
+00:21:15,250 --> 00:21:20,130
+لضمان التوافق بين الفئات جميعًا. وهذا كله بيساعدنا
+
+262
+00:21:20,130 --> 00:21:24,390
+على العمل المشترك بينها. هذا كله عن طريق العمليات
+
+263
+00:21:24,390 --> 00:21:27,950
+التربوية. لذا هنا بنركز على اللي هو التثقيف
+
+264
+00:21:27,950 --> 00:21:34,370
+والتوعية، العمليات التثقيفية والتوعوية للجمهور. واضح؟
+
+265
+00:21:34,370 --> 00:21:41,850
+في طريق التنظيم المجتمعي أيضا
+
+266
+00:21:41,850 --> 00:21:46,030
+في عندنا عمليات أخرى، اللي هي عمليات إنمائية أو
+
+267
+00:21:46,030 --> 00:21:52,300
+إنشائية. العمليات الإنمائية والإنشائية. إذا فعلا أربع
+
+268
+00:21:52,300 --> 00:21:57,040
+عمليات: العمل بين الجماعات، الأولى، العمل الإداري،
+
+269
+00:21:57,040 --> 00:22:02,440
+العمليات الإدارية، العمليات التربوية والتثقيفية، ثم
+
+270
+00:22:02,440 --> 00:22:06,900
+بعد ذلك العمليات الإنمائية والإنشائية. وهذه تقوم
+
+271
+00:22:06,900 --> 00:22:12,420
+بدور التنمية من أجل إحداث التغيير الاجتماعي داخل
+
+272
+00:22:12,420 --> 00:22:17,050
+المجتمع. وفي فرق بين التغير والتغيير. إحداث تغيير
+
+273
+00:22:17,050 --> 00:22:20,990
+اجتماعي، لأن التغيير هو مقصود، تتدخل فيه الإرادة
+
+274
+00:22:20,990 --> 00:22:26,350
+الإنسانية. تغيير مقصود من قبل الإنسان. التغير قد
+
+275
+00:22:26,350 --> 00:22:30,330
+يكون أحيانًا تغير طبيعي وتلقائي. لكن هنا في تنظيم
+
+276
+00:22:30,330 --> 00:22:34,470
+المجتمع، نحن نقصد بالتغير اللي هو التغيير الاجتماعي،
+
+277
+00:22:34,860 --> 00:22:38,480
+التغيير المقصود، نعم، إحداثه. فهنا ما يقصد به ليه
+
+278
+00:22:38,480 --> 00:22:42,420
+عمليات إنمائية أو إنشائية، اللي هي إحداث التغيير
+
+279
+00:22:42,420 --> 00:22:47,900
+المطلوب. واضح؟ والتنمية، والعمل على إحداث التنمية
+
+280
+00:22:47,900 --> 00:22:51,640
+داخل المجتمع. والتنمية هذه اللي بتسبب اللي هي
+
+281
+00:22:51,640 --> 00:22:56,130
+التغيير الاجتماعي إلى الأفضل. لأن التنمية دائمًا هي
+
+282
+00:22:56,130 --> 00:23:01,210
+إحداث تغييرات في الجوانب الاجتماعية. قد تكون هذه
+
+283
+00:23:01,210 --> 00:23:05,790
+الجوانب، يعني، إيجابية. أما التغير، أحيانًا ممكن التغير
+
+284
+00:23:05,790 --> 00:23:10,170
+التلقائي، قد يكون إيجابي وقد يكون سلبي. واضح؟ لكن
+
+285
+00:23:10,170 --> 00:23:14,630
+التنمية، نحن نقصد بها إحداث تغييرات مقصودة، إحداث
+
+286
+00:23:14,630 --> 00:23:18,570
+تغييرات مقصودة، جذرية داخل المجتمع. وهذا ما يقصده
+
+287
+00:23:18,570 --> 00:23:21,950
+اللي هي العمليات الإنمائية والإنشائية، من أجل إحداث
+
+288
+00:23:21,950 --> 00:23:26,240
+تغييرات داخل المجتمع إلى الأفضل، من خلال التنمية
+
+289
+00:23:26,240 --> 00:23:32,340
+وبرامج التنمية داخل المجتمع. تغيير، نعم، إحداث تغيير
+
+290
+00:23:32,340 --> 00:23:36,760
+نعم، ما يخالف تغير، لكن نقصد به نحن التغيير، التغيير
+
+291
+00:23:36,760 --> 00:23:41,600
+إلى الأفضل، اللي يبدأ عن طريق التخطيط المقصود، العام
+
+292
+00:23:41,600 --> 00:23:47,760
+المقصود، يعني التخطيط، يقوم على التخطيط. طيب
+
+293
+00:23:47,760 --> 00:23:51,460
+نعم، خلصنا.
+
+294
+00:23:54,220 --> 00:23:57,940
+يعني نكون بذلك، يعني انتهينا من دور المنظم
+
+295
+00:23:57,940 --> 00:24:01,960
+الاجتماعي، كما وضحوا لنا، أنه استطار في العمليات
+
+296
+00:24:01,960 --> 00:24:05,640
+الأربعة، اللي هي العمل بين الجماعات، وهذا بيقوم على
+
+297
+00:24:05,640 --> 00:24:10,000
+الدور التنسيقي بين الجماعات وبين الهيئات والمؤسسات
+
+298
+00:24:10,000 --> 00:24:13,600
+الاجتماعية داخل المجتمع، وأيضا بيقوم على الدور
+
+299
+00:24:20,200 --> 00:24:26,320
+العمل الإداري،يقوم
+
+300
+00:24:26,320 --> 00:24:31,280
+على تشغيل وظائف الإدارة، مثل التنسيق، والتخطيط،
+
+301
+00:24:31,280 --> 00:24:36,880
+وتوزيع المسؤوليات، وغير ذلك داخل المجتمع، والإشراف،
+
+302
+00:24:36,880 --> 00:24:42,420
+والتوجيه. هذه كلها أيضًا عمليات إدارية. العملية
+
+303
+00:24:42,420 --> 00:24:47,600
+الثالثة هي العملية التثقيفية، العملية اللي هي
+
+304
+00:24:47,600 --> 00:24:52,460
+التربوية، التثقيفية التي تقوم على تثقيف الجمهور،
+
+305
+00:24:52,460 --> 00:24:57,980
+والعملية الرابعة هي العملية الإنمائية التي تقوم
+
+306
+00:24:57,980 --> 00:25:02,020
+على اللي هو إحداث التنمية داخل المجتمع من أجل
+
+307
+00:25:02,020 --> 00:25:06,360
+التغيير الاجتماعي المقصود، إلى الأفضل وإلى الأحسن.
+
+308
+00:25:06,950 --> 00:25:13,090
+لكون في هذه المحاضرة قد، يعني، تحدثنا عن العوامل
+
+309
+00:25:13,090 --> 00:25:16,390
+اللي أَوْدت الاختلاف، الكتاب، حول صياغة دور المنظم
+
+310
+00:25:16,390 --> 00:25:20,830
+الاجتماعي، ثم تحدثنا على نموذج من النماذج الغربية
+
+311
+00:25:20,830 --> 00:25:26,150
+حول دور المنظم الاجتماعي، وهو دور، وجهة نظر الـ
+
+312
+00:25:26,150 --> 00:25:30,210
+Neustatter. إن شاء الله في المحاضرة القادمة نكمل
+
+313
+00:25:30,210 --> 00:25:37,550
+وجهة نظر علماء الغرب، ثم ننتقل إلى وجهة نظر العلماء
+
+314
+00:25:37,550 --> 00:25:41,610
+العرب حول وجهة…حول دور المنظم الاجتماعي. في أي
+
+315
+00:25:41,610 --> 00:25:46,570
+سؤال الآن؟ طيب، إلى أن نلتقي في المحاضرة القادمة، لكم
+
+316
+00:25:46,570 --> 00:25:50,630
+من كل تحية وتقدير، والسلام عليكم ورحمة الله
+
+317
+00:25:50,630 --> 00:25:51,850
+وبركاته.
diff --git a/PL9fwy3NUQKwZ8bh257eMcqZ22bPBOhO8z/iICaAA1ruDo_raw.srt b/PL9fwy3NUQKwZ8bh257eMcqZ22bPBOhO8z/iICaAA1ruDo_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..a23807b5ab906eb251a7f32698eaf0ae084fb2da
--- /dev/null
+++ b/PL9fwy3NUQKwZ8bh257eMcqZ22bPBOhO8z/iICaAA1ruDo_raw.srt
@@ -0,0 +1,1268 @@
+1
+00:00:21,010 --> 00:00:25,370
+بسم الله الرحمن الرحيم الحمد لله رب العالمين
+
+2
+00:00:25,370 --> 00:00:30,050
+والصلاة والسلام على أشرف المرسلين سيدنا محمد وعلى
+
+3
+00:00:30,050 --> 00:00:35,470
+آله وصحبه أجمعين بنات الطالبات السلام عليكم ورحمة
+
+4
+00:00:35,470 --> 00:00:41,370
+الله وبركاته في المحاضرة السابقة كنا نتحدث عن دور
+
+5
+00:00:41,370 --> 00:00:47,970
+المنظم الاجتماعي وتحدثنا عن مفهوم الدور ومكونات
+
+6
+00:00:47,970 --> 00:00:54,140
+الدور ونظرية الدورفي هذه المحاضرة سنتعرف على
+
+7
+00:00:54,140 --> 00:00:59,360
+العوامل اللي أدت لاختلاف الكتاب عند صياغتهم لأدوار
+
+8
+00:00:59,360 --> 00:01:05,240
+المنظم الاجتماعي وطبعا في عدد من العوامل سنناقش
+
+9
+00:01:05,240 --> 00:01:09,860
+هذه .. سنذكر هذه العوامل ونناقش كل عامل على حدد
+
+10
+00:01:10,520 --> 00:01:14,760
+طبعا من هذه العوامل اللي أدت إلى اختلاف الكتاب عند
+
+11
+00:01:14,760 --> 00:01:20,360
+كتابتهم وصياغتهم لدور المنظم الاجتماعي أول شيء
+
+12
+00:01:20,360 --> 00:01:25,600
+اختلاف مستوى التجريد ثم اختلاف النماذج ثم اختلاف
+
+13
+00:01:25,600 --> 00:01:30,020
+المراحل التاريخية ثم بعد ذلك اختلاف مجالات العمل
+
+14
+00:01:30,020 --> 00:01:35,740
+ثم اختلاف الظروف والتيارات الاجتماعية السائدة هذه
+
+15
+00:01:35,740 --> 00:01:41,830
+النقاط كلها سنناقشها نقطة نقطةلكي نتعرف على
+
+16
+00:01:41,830 --> 00:01:46,530
+الأسباب اللي أدت إلى اختلاف الكتاب حول صياغتهم
+
+17
+00:01:46,530 --> 00:01:52,130
+لدور المنظم الاجتماعي سناخد النقطة الأولى و هي
+
+18
+00:01:52,130 --> 00:01:57,310
+اختلاف مستوى التجريد طبعا ايش يعني اختلاف مستوى
+
+19
+00:01:57,310 --> 00:02:01,740
+التجريد؟ نعم يا بنتفأفضل بعض الكتاب القيام حسب ما
+
+20
+00:02:01,740 --> 00:02:05,720
+يقوم بالمعظم من خلال عمله اليومي في مجال أو مجالات
+
+21
+00:02:05,720 --> 00:02:10,180
+متقاربة من خلال تصنيف تبت لانش تحت بعض العنوان
+
+22
+00:02:10,180 --> 00:02:14,580
+العامة ثم التواصل إلى دور المعظم ليعرف دور الوصفة
+
+23
+00:02:14,610 --> 00:02:17,990
+بالضبط يعني من خلال عمله من خلال عمل المنظر في
+
+24
+00:02:17,990 --> 00:02:22,390
+المؤسسات الاجتماعية بيحصر دوره و خاصة المؤسسات
+
+25
+00:02:22,390 --> 00:02:26,670
+المتشابهة في المجال و في ميدان العمل الواحد في
+
+26
+00:02:26,670 --> 00:02:29,930
+مجال الخدمة الاجتماعية الواحد أو ميدان العمل
+
+27
+00:02:29,930 --> 00:02:34,310
+الواحد للخدمة الاجتماعية فبقوم المنظم يحصر الأدوار
+
+28
+00:02:34,310 --> 00:02:37,430
+التي يقوم بها في هذا المجال من خلال عدد من
+
+29
+00:02:37,430 --> 00:02:40,870
+المؤسساتالتي يعملون فيها اللي هم الأقصائيين
+
+30
+00:02:40,870 --> 00:02:45,150
+الاجتماعيين فكل واحد بيكتب ايه الوصف الدور الوصفي
+
+31
+00:02:45,150 --> 00:02:50,310
+لما يقوم بيه داخل المؤسسة من أعمال في مجال واحد
+
+32
+00:02:50,310 --> 00:02:55,730
+ومن مجموع هذه الكتابات للدور الوصفي ممكن نصل إلى
+
+33
+00:02:55,730 --> 00:03:01,010
+كتابة معينة أو إلى وصف معين لدور المنظم الاجتماعي
+
+34
+00:03:01,010 --> 00:03:05,650
+من خلال الصياغة الوصفية لدور المنظم الاجتماعي في
+
+35
+00:03:05,650 --> 00:03:11,130
+مجالات العمل المتشابهةعندما يقوم بها في ميدان طريق
+
+36
+00:03:11,130 --> 00:03:15,810
+التنظيم المجتمع أو و هكذا فلذلك من هنا بيحصر الدور
+
+37
+00:03:15,810 --> 00:03:19,470
+الوصفي لدور الاقتصادي الاجتماعي عن طريق حصر كل ما
+
+38
+00:03:19,470 --> 00:03:23,750
+يقوم بيه من أعمال ثم بعد ذلك قد يعني يجعل من هذا
+
+39
+00:03:23,750 --> 00:03:29,690
+الوصف اللي هو الدور الكامل والدور يعني بنسميه قد
+
+40
+00:03:29,690 --> 00:03:34,130
+يكون الدور الذي له طابع تجريدي الذي يقوم بوصفه
+
+41
+00:03:34,130 --> 00:03:39,280
+المنظم الاجتماعي وفي هذه الحالةيعني يوضع من ضمن
+
+42
+00:03:39,280 --> 00:03:44,240
+الدور المنظم الاجتماعي في طريق التنظيم المجتمع في
+
+43
+00:03:44,240 --> 00:03:48,140
+مجال معين من مجالات طريق التنظيم المجتمع
+
+44
+00:03:56,270 --> 00:03:59,890
+طيب خلاص فهمنا النقطة الأولى نأتي إلى النقطة
+
+45
+00:03:59,890 --> 00:04:03,970
+الثانية اختلاف النماذج ايوة يا بنتي بسبب صعوبة
+
+46
+00:04:03,970 --> 00:04:07,750
+عملية التجريد اعتمد اكتر المنظمين قرروا انهم
+
+47
+00:04:07,750 --> 00:04:10,950
+يعتمدوا نموذج واحد عشان هم يقدروا يعملوا عملية
+
+48
+00:04:10,950 --> 00:04:15,050
+التجريد فكل واحد من المنظمين اعتمد بنموذج اللي هو
+
+49
+00:04:15,050 --> 00:04:18,750
+يعني بعتقد ان هو الأفضل فمنهم اعتمد نظرية خدمة
+
+50
+00:04:18,750 --> 00:04:21,970
+الفرد او نموذج خدمة الفرد ومنهم اعتمد نموذج خدمة
+
+51
+00:04:21,970 --> 00:04:25,960
+الجامعة وبهك اختلفوا في ..نعم اختلفوا في الكتابات،
+
+52
+00:04:25,960 --> 00:04:30,740
+نعم، إذا من ال .. اللي هما اختلافهم في اللي هي عمل
+
+53
+00:04:30,740 --> 00:04:34,940
+النماذج أو كتابة النماذج اللي هي و لذلك اختلفوا
+
+54
+00:04:34,940 --> 00:04:38,940
+هنا في النموذج اللي اتخذوه في عملية التجريد فهذا
+
+55
+00:04:38,940 --> 00:04:43,480
+يعتبر برضه سبب من أسباب اختلافهم في صياغة دور
+
+56
+00:04:43,480 --> 00:04:47,560
+المنظم الاجتماعي، إذا السبب الأول هو اختلافهم من
+
+57
+00:04:47,560 --> 00:04:53,000
+حيث التجريد، السبب الثاني هو من حيث النماذج، طيب
+
+58
+00:04:53,810 --> 00:04:57,450
+طبعا بعض الكتاب بيفضل القياس أحيانا على نموذج
+
+59
+00:04:57,450 --> 00:05:03,270
+أخصائي خدمة الفرد أو أخصائي أو مثلا أحيانا بياخد
+
+60
+00:05:03,270 --> 00:05:07,310
+زي نموذج دور روس و البعض الآخر طبعا نموذج دور
+
+61
+00:05:07,310 --> 00:05:11,290
+روسنا اللي هي التنمية دور التنموي في طريق تنظيم
+
+62
+00:05:11,290 --> 00:05:16,090
+المجتمع لأنه ربط طريق تنظيم المجتمع بالتنمية أيضا
+
+63
+00:05:16,090 --> 00:05:20,290
+في نموذج آخر خدمة الجماعة مثل ما هناخد دور ال New
+
+64
+00:05:20,290 --> 00:05:26,160
+Stater اللي ربطه بخدمة الجماعةوهكذا فلذلك من هنا
+
+65
+00:05:26,160 --> 00:05:31,660
+اختلفت اللي هي النماذج عند صياغة دور أو اختلفت
+
+66
+00:05:31,660 --> 00:05:35,960
+اختلف الكتاب في صياغ دور المنظم الاجتماعي عن طريق
+
+67
+00:05:35,960 --> 00:05:40,340
+اختلافهم في اللي هي النماذج المختلفة سواء كان
+
+68
+00:05:40,340 --> 00:05:44,720
+نموذج خدمة الفرد أو نموذج خدمة الجماعة أو حتى في
+
+69
+00:05:44,720 --> 00:05:48,800
+نماذج في طريقة تنظيم المجتمع عند ربط طريقة تنظيم
+
+70
+00:05:48,800 --> 00:05:53,690
+المجتمع بالتنمية الاجتماعية مثل دور روسفي عندنا
+
+71
+00:05:53,690 --> 00:05:59,750
+الآن سبب آخر أيضًا لاختلاف الكتاب أو العلماء لكتاب
+
+72
+00:05:59,750 --> 00:06:04,230
+دور محدد لدور المنظم الاجتماعي ما هو هذا السبب
+
+73
+00:06:04,230 --> 00:06:10,810
+الثالث؟ سبب
+
+74
+00:06:10,810 --> 00:06:16,650
+ثالث سبب واضح اللي هي المراحل التاريخية اللي ظهرت
+
+75
+00:06:16,650 --> 00:06:18,090
+فيها طريقة تنظيم المجتمع
+
+76
+00:06:20,680 --> 00:06:25,520
+وكل مرحلة من هذه المراحل أدت إلى صياغ الدورة انتم
+
+77
+00:06:25,520 --> 00:06:28,660
+ما عرفتم مراحل طريقة تنظيم المجتمع مش عندنا أربع
+
+78
+00:06:28,660 --> 00:06:34,500
+مراحل مرحلة التنسيقية أولا ثم المرحلة التخطيطية ثم
+
+79
+00:06:34,500 --> 00:06:40,580
+مرحلة التنمية ثم مرحلة الدفاع في كل مرحلة من هذه
+
+80
+00:06:40,580 --> 00:06:46,480
+المراحل كان هناك دور للمنظم الاجتماعيولذلك اختلف
+
+81
+00:06:46,480 --> 00:06:51,160
+الكتاب في صياغة الدور حسب المرحلة التاريخية لتطور
+
+82
+00:06:51,160 --> 00:06:57,450
+طريق تنظيم المجتمعواضح فلذلك من هنا يعني في أدوار
+
+83
+00:06:57,450 --> 00:07:01,290
+مختلفة لإنت عارفين إحنا طريق التنظيم المجتمع ظهرت
+
+84
+00:07:01,290 --> 00:07:07,610
+عام 1946 ظهرت بالمرحلة التنسيقية ثم التخطيطية ثم
+
+85
+00:07:07,610 --> 00:07:12,110
+التنمية ثم الدفاع في كل مرحلة من هذه المراحل ظهر
+
+86
+00:07:12,110 --> 00:07:16,910
+دور للمنظم الاجتماعي دور في مرحلة التنسيق دور في
+
+87
+00:07:16,910 --> 00:07:21,450
+مرحلة التخطيط كمخطط دور في مرحلة التنمية كدور ال
+
+88
+00:07:21,450 --> 00:07:26,010
+.. ال ..الممكن والمنمي اللي هو في التنمية ودور
+
+89
+00:07:26,010 --> 00:07:30,030
+المدافع وها كذا إذا من هنا اختلف الكتاب حسب
+
+90
+00:07:30,030 --> 00:07:34,710
+المراحل التاريخية في كتابتهم لدور المنظم الاجتماعي
+
+91
+00:07:34,710 --> 00:07:41,910
+طيب في عندنا أيضاهذه كلها يعني اللي احنا ذكرناها،
+
+92
+00:07:41,910 --> 00:07:45,250
+الدور المنسق، الدور المخطط، الدور المنمي، الدور
+
+93
+00:07:45,250 --> 00:07:50,710
+المدافع أو الثورة، اللي هي آخر الأدوار، وهي الأخر
+
+94
+00:07:50,710 --> 00:07:56,570
+مرحلة من مراحل تطور طريق تنظيم المجتمع في عندنا
+
+95
+00:07:56,570 --> 00:08:01,670
+سبب آخر أيضا من الأسباب اللي أدت إلى اختلاف الكتاب
+
+96
+00:08:01,670 --> 00:08:05,770
+عند صيغتي من الدور المنظم، نعم يا بنتيفي أغلب
+
+97
+00:08:05,770 --> 00:08:10,070
+الكتاب يختلفوا في سياغتهم لهذا النموذج في نوع معين
+
+98
+00:08:10,070 --> 00:08:13,790
+من الأجزة أو مستوى من المستويات، فروث .. روث اتأثر
+
+99
+00:08:13,790 --> 00:08:18,650
+بكتابات تنمية المشارع المحل الصغير، أما روبرت و ..
+
+100
+00:08:18,650 --> 00:08:21,710
+و ال newsletter اتأثر بمجالات .. مجالس الهيئات
+
+101
+00:08:21,710 --> 00:08:25,290
+الاجتماعية، أما كتابات البروسر و غيرهم من الكتاب
+
+102
+00:08:25,290 --> 00:08:31,240
+اتأثربقدر، إذن هذا سبب أيضا من السباب اختلاف
+
+103
+00:08:31,240 --> 00:08:35,680
+الكتاب عند صياغتهم لدور المنظم الاجتماعي وهو
+
+104
+00:08:35,680 --> 00:08:40,700
+اختلاف مجالات العمل فكل كاتب أو كل عالم من علماء
+
+105
+00:08:40,700 --> 00:08:44,920
+طريق التنظيف المجتمع صاغ دور المنظم حسب المجال
+
+106
+00:08:44,920 --> 00:08:50,020
+الذي يعمل فيه أو المجال الذي يكتب من خلاله مثل دور
+
+107
+00:08:50,020 --> 00:08:56,280
+Ross يعني صياغته لدور المنظم اللي هي ربطه بالتنمية
+
+108
+00:08:56,280 --> 00:09:00,970
+الاجتماعيةربط طريقة دور المنظم الاجتماعي في طريقة
+
+109
+00:09:00,970 --> 00:09:05,750
+تنظيم المجتمع ربطه بدور التنمية الاجتماعية و الـ
+
+110
+00:09:05,750 --> 00:09:08,590
+New Stater ربطه بدور .. ربط دور ماذا؟ ال New
+
+111
+00:09:08,590 --> 00:09:11,890
+Stater بالتنسيق اللي هي التنسيق بين الجامعات
+
+112
+00:09:11,890 --> 00:09:16,490
+التنسيق بين الجامعات و مجالس الهيئات الاجتماعية
+
+113
+00:09:16,490 --> 00:09:23,570
+المختلفة أيضا دور Grosser ربطه أيضا بالحقوق
+
+114
+00:09:23,570 --> 00:09:29,550
+المدنية و العمل مع الفقراء و هكذاإذا كل كاتب ربط
+
+115
+00:09:29,550 --> 00:09:35,290
+دور المنظم حسب .. حسب ما كان يعني يهوى و يعمل فيه
+
+116
+00:09:35,290 --> 00:09:38,750
+في المجال العمل اللي كان يعمل فيه فربط دور المنظم
+
+117
+00:09:38,750 --> 00:09:46,160
+بمجال العمل اللي يعمل فيه و يكتب فيهفي عندنا
+
+118
+00:09:46,160 --> 00:09:50,900
+اختلاف الظروف والتيارات الاجتماعية كذلك هذا سبب من
+
+119
+00:09:50,900 --> 00:09:56,140
+أسباب اختلاف الكتاب حول كتابتهم لدور المنظم
+
+120
+00:09:56,140 --> 00:10:00,800
+الاجتماعي والوصول إلى دور واحد فلذلك من هنا
+
+121
+00:10:00,800 --> 00:10:05,220
+اختلفوا حول الأدوار فهذا اختلاف الظروف والتيارات
+
+122
+00:10:05,220 --> 00:10:08,620
+الاجتماعية السائدة طبعا
+
+123
+00:10:10,110 --> 00:10:14,870
+في أحيانا تيارات ديمقراطية، تيارات دكتاتورية،
+
+124
+00:10:14,870 --> 00:10:20,770
+تيارات رأسمالية، تيارات اشتراكية، واضح أيضا احنا
+
+125
+00:10:20,770 --> 00:10:25,170
+في مجتمعنا العربي كذلك في تيارات إسلامية، قومية،
+
+126
+00:10:25,170 --> 00:10:31,420
+وهكذاإذن التيارات هذه لها كذلك تأثير عند صياغ دور
+
+127
+00:10:31,420 --> 00:10:35,940
+المنظم الاجتماعي في المجتمع فلذلك لابد أن نلمب هذه
+
+128
+00:10:35,940 --> 00:10:40,400
+التيارات المختلفة عند دراستنا لصياغ دور المنظم
+
+129
+00:10:40,400 --> 00:10:43,600
+الاجتماعي ونعرف دور المنظم الاجتماعي في المجتمع
+
+130
+00:10:43,600 --> 00:10:47,300
+الاشتراكي ما هو ودور المنظم الاجتماعي في المجتمع
+
+131
+00:10:47,300 --> 00:10:51,490
+الرئاسمالي ما هومجتمع الغرب الرأسمالي ونعرف دور
+
+132
+00:10:51,490 --> 00:10:55,250
+المنظم الاجتماعي في مجتمعنا العربي الإسلامي كذلك
+
+133
+00:10:55,250 --> 00:11:00,170
+ما هو فلذلك من هنا الكتاب يختلفون في صياغتهم لدور
+
+134
+00:11:00,170 --> 00:11:06,970
+المنظم حسب التيارات السياسية والعقائدية وأيضا كل
+
+135
+00:11:06,970 --> 00:11:11,030
+هذه التيارات يعني كانت سببا في اختلافهم عند
+
+136
+00:11:11,030 --> 00:11:16,010
+صياغتهم لدور المنظم الاجتماعي طيب
+
+137
+00:11:18,360 --> 00:11:23,220
+في عندنا أيضًا يعني مثلًا دور المنظم الاجتماعي
+
+138
+00:11:23,220 --> 00:11:28,500
+يعني هذا كان في مرحلة أخيرة من مراحل تطور طريق
+
+139
+00:11:28,500 --> 00:11:32,980
+تنظيم المجتمع عند قيام اللي هم ثورة الفقراء
+
+140
+00:11:32,980 --> 00:11:37,200
+والزنوج في أمريكا فلذلك ظهر دور المنظم الاجتماعي
+
+141
+00:11:37,200 --> 00:11:41,360
+في السبعينات وفي حرب فيتنام عندما صاروا الشباب على
+
+142
+00:11:41,360 --> 00:11:45,960
+حكومتهم من أجل الاعتراض على الحربالفيتنامية الحرب
+
+143
+00:11:45,960 --> 00:11:50,540
+بين أمريكا وفيتنام فثار الشباب فأصبح هناك ظهر دور
+
+144
+00:11:50,540 --> 00:11:54,480
+المدافع في أمريكا دور المدافع في طريق تنظيم
+
+145
+00:11:54,480 --> 00:11:58,460
+المجتمع وفي الخدمة الاجتماعية ولذلك الذنوج كذلك
+
+146
+00:11:58,460 --> 00:12:04,080
+ثورة الذنوج ضد البيض هذا جعل أن يظهر هناك دور جديد
+
+147
+00:12:04,080 --> 00:12:08,280
+لطريق تنظيم المجتمع وهو دور المدافع عن حقوق هؤلاء
+
+148
+00:12:08,280 --> 00:12:12,920
+المظلومين الذنوج من البيض طبعا لأن الذنوجهم أصل
+
+149
+00:12:12,920 --> 00:12:17,340
+البلاد في أمريكاأصل البلاد في أمريكا هم الزنوج مثل
+
+150
+00:12:17,340 --> 00:12:21,520
+اللي احنا في فلسطين أصل الفلسطينيين في فلسطين هم
+
+151
+00:12:21,520 --> 00:12:26,500
+الفلسطينيون فلذلك من هنا هناك قد يكون صراع ما بين
+
+152
+00:12:26,500 --> 00:12:30,800
+الحق والباطل ما بين الفئات الضعيفة والفئات القوية
+
+153
+00:12:30,800 --> 00:12:34,280
+المستبدة فلذلك من هنا ظهرت الأدوار في الخدمة
+
+154
+00:12:34,280 --> 00:12:38,580
+الاجتماعية اللي هي دور المدافع كذلك الفقراء
+
+155
+00:12:39,030 --> 00:12:43,890
+ومطالباتهم بحقوقهم من الأغنياء وغير ذلك هذا كله
+
+156
+00:12:43,890 --> 00:12:49,550
+جعل لدور المنظم الاجتماعي كدور مدافع في آخر مراحل
+
+157
+00:12:49,550 --> 00:12:54,130
+تطور طريق تنظيم المجتمع يظهر بوضوح منذ السبعينات
+
+158
+00:12:54,130 --> 00:12:58,550
+في أمريكا وبعد ذلك انتشر هذا في العالم الغربي
+
+159
+00:12:58,550 --> 00:13:02,010
+والعالم العربي كذلك من خلال النقابات المهنية
+
+160
+00:13:02,010 --> 00:13:06,790
+والجمعيات المطالبة بحقوق الإنسان والنقابات التي
+
+161
+00:13:06,790 --> 00:13:12,170
+تطالب بحقوق العاملينفيها هذا كله يعني بركز لنا على
+
+162
+00:13:12,170 --> 00:13:17,250
+دور الإيه المدافع كدور للمنظم الاجتماعي اللي هو
+
+163
+00:13:17,250 --> 00:13:21,450
+دور المدافع في طريق تنظيم المجتمع وفي الخدمة
+
+164
+00:13:21,450 --> 00:13:23,170
+الاجتماعية كمهنة
+
+165
+00:13:27,350 --> 00:13:33,670
+في عندنا يعني نستعرض الأراء الآن يعني ممكن احنا
+
+166
+00:13:33,670 --> 00:13:38,470
+عرفنا العوامل اللي قدت الاختلاف الكتاب أو اختلاف
+
+167
+00:13:38,470 --> 00:13:43,790
+العلماء أو الاختلاف يعني المختصين في تنظيم المجتمع
+
+168
+00:13:43,790 --> 00:13:47,350
+عند صياغته من دور المنظمة الآن بدنا ناخد وجهة نظر
+
+169
+00:13:47,350 --> 00:13:52,430
+الغرب ووجهة نظر العرب يعني الكتاب العرب العلماءفي
+
+170
+00:13:52,430 --> 00:13:58,710
+دور المنظم الاجتماعي فسناخد بعض العلماء الغرب عند
+
+171
+00:13:58,710 --> 00:14:03,670
+صياغتهم لدور المنظم الاجتماعي وبعد ذلك ان شاء الله
+
+172
+00:14:03,670 --> 00:14:10,630
+سنتناول دور اللي هو العلماء والمختصينمن أساتذتنا
+
+173
+00:14:10,630 --> 00:14:14,370
+رحمة الله عليهم من توفاه الله وأيضا رحمة الله على
+
+174
+00:14:14,370 --> 00:14:18,870
+من هو موجود فالرحمة تجوز على من توفاه الله وعلى من
+
+175
+00:14:18,870 --> 00:14:23,770
+هو حي فلذلك سنستعرض أيضا بعض الأراء بإذن الله
+
+176
+00:14:23,770 --> 00:14:27,330
+تعالى في هذه المحاضرة ربما نستعرض بعض الأراء
+
+177
+00:14:27,330 --> 00:14:33,820
+للعلماء الأجانب حول دور المنظم الاجتماعيطبعا عندنا
+
+178
+00:14:33,820 --> 00:14:39,540
+كوجهة نظر الغربية سناخذ نظر .. وجهة نظر ال New
+
+179
+00:14:39,540 --> 00:14:42,980
+Stater يعني يكفي لو أخدنا ال New Stater في البداية
+
+180
+00:14:42,980 --> 00:14:47,380
+ك .. في هذه المحاضرة ثم في المحاضرات القادمة سناخد
+
+181
+00:14:47,380 --> 00:14:51,640
+باقي العلماء سواء من الأجانب أو العرب طبعا ال New
+
+182
+00:14:51,640 --> 00:15:00,340
+Stater هذا طبعا قدم بحث في العمل بين الجماعاتوأشار
+
+183
+00:15:00,340 --> 00:15:04,700
+في هذا البحث قال أن تنظيم المجتمع بتضمن ثلاث
+
+184
+00:15:04,700 --> 00:15:09,720
+عمليات أساسية ما هي هذه العمليات الأساسية اللي
+
+185
+00:15:09,720 --> 00:15:13,760
+بتضمنها اللي هو العمل بين الجامعات اللي هي تنظيم
+
+186
+00:15:13,760 --> 00:15:19,360
+المجتمع بتضمن ثلاث عمليات أساسية طبعا هذه العمليات
+
+187
+00:15:19,360 --> 00:15:24,140
+هي العمل بين الجامعات والعمليات الإدارية والتربوية
+
+188
+00:15:24,140 --> 00:15:32,660
+وكذلك العمليات الإلمائية أو الإنشائيةنعم طيب خلاص
+
+189
+00:15:32,660 --> 00:15:37,120
+وين الرابعة؟ طب
+
+190
+00:15:37,120 --> 00:15:42,580
+ما احنا قلناها نقولها ثاني طيب طيب في عندنا ثلاث
+
+191
+00:15:42,580 --> 00:15:52,640
+عمليات هي أربعة؟ ثلاثة احنا
+
+192
+00:15:52,640 --> 00:15:58,280
+قلنا أشار في هذا البحثبأن تنظيم المجتمع يتضمن ثلاث
+
+193
+00:15:58,280 --> 00:16:03,940
+عمليات واحدة من هذه العمليات فقط يمكن اعتبارها من
+
+194
+00:16:03,940 --> 00:16:07,900
+العمليات التي تتم في نطاق الخدمة الاجتماعية وهي
+
+195
+00:16:07,900 --> 00:16:13,320
+العمل بين الجامعات وأشار إلى أن المنظم يستطيع
+
+196
+00:16:13,320 --> 00:16:18,200
+مزاولة العمليات الأخرى، ما هي العمليات الأخرى؟اللي
+
+197
+00:16:18,200 --> 00:16:21,600
+هي العمليات الإدارية والتربوية مش احنا بنمارس
+
+198
+00:16:21,600 --> 00:16:25,880
+ونقرأ إدارة احنا ولا إدارة المؤسسات الاجتماعية إذن
+
+199
+00:16:25,880 --> 00:16:30,540
+هذه فيها ممارسة للإدارة وبنطبق خدمة الاجتماعية من
+
+200
+00:16:30,540 --> 00:16:35,600
+خلال معرفتنا بالأدارة ووظائف الإدارة المختلفة إذن
+
+201
+00:16:35,600 --> 00:16:40,260
+هذه هي العملية إذن من هنا المنظم بيزاول أيضا اللي
+
+202
+00:16:40,260 --> 00:16:46,080
+هي العمليات الإدارية والتربوية واضح كذلك أضاف أن
+
+203
+00:16:46,080 --> 00:16:50,680
+يستطراللي هي العمليات الإنمائية أو الإنشائية
+
+204
+00:16:50,680 --> 00:16:56,360
+العمليات الإنمائية للتنموية و طبعا هذا اتأثر ان
+
+205
+00:16:56,360 --> 00:17:01,480
+يستثر بمين بروس عن تنظيم المجتمع و ربط فيها بين
+
+206
+00:17:01,480 --> 00:17:06,540
+تنمية المجتمع و تنظيم المجتمع ربط فيها بين تنظيم
+
+207
+00:17:06,540 --> 00:17:12,600
+المجتمع و تنمية المجتمع فلذلك روس ركز على تنمية
+
+208
+00:17:12,600 --> 00:17:17,150
+المجتمعوهنا اتأثر بيه مين؟ اللي هو Newsletter برضه
+
+209
+00:17:17,150 --> 00:17:22,030
+بروس لربط بين التنمية، تنمية المجتمع وتنظيم
+
+210
+00:17:22,030 --> 00:17:27,770
+المجتمع طيب،
+
+211
+00:17:27,770 --> 00:17:30,950
+إذا من هنا أدوار المنظم من وجهة نظر ال Newsletter
+
+212
+00:17:30,950 --> 00:17:34,390
+طبعا
+
+213
+00:17:34,390 --> 00:17:38,530
+أول شيء العمل بين الجامعات وهذه عملية تنسيقية يعني
+
+214
+00:17:38,530 --> 00:17:43,540
+هنا أركز على الدور التنسيقيبين الجماعات مع ممثلي
+
+215
+00:17:43,540 --> 00:17:50,280
+الهيئات في المنظمة وأيضا قال
+
+216
+00:17:50,280 --> 00:17:54,200
+إنه لابد من تحسين العلاقات بين جماعات المجتمع
+
+217
+00:17:54,200 --> 00:17:58,240
+المحلي والتأكد من تمثيل كل الجماعات الأهم في
+
+218
+00:17:58,240 --> 00:18:02,460
+المجتمع وعندما نتأكد من تمثيل كل الجماعات في
+
+219
+00:18:02,460 --> 00:18:05,540
+المجتمع نستطيع أن نتعامل مع المجتمع لأن احنا لا
+
+220
+00:18:05,540 --> 00:18:09,840
+نتعامل مع المجتمع ككل إلا من خلال ممثليه من خلال
+
+221
+00:18:09,840 --> 00:18:14,240
+القادةمن خلال من يتخبوا من المجتمع ومن خلال من
+
+222
+00:18:14,240 --> 00:18:17,840
+يمثلون المجتمع هكذا دور رحنا في طريقة تنظيم
+
+223
+00:18:17,840 --> 00:18:24,020
+المجتمع فإذا اتأثر هنا اللي هو أكد على دور المنظم
+
+224
+00:18:24,020 --> 00:18:29,240
+اللي هو أول شيء اللي هو العمل بين الجماعات وأيضا
+
+225
+00:18:29,240 --> 00:18:33,840
+ركز على اللي هم كموثلي للوحدات الاجتماعية اللي هي
+
+226
+00:18:33,840 --> 00:18:38,100
+بين منظمات الجماعية منظمات الجماعات المختلفة اللي
+
+227
+00:18:38,100 --> 00:18:43,530
+بتعمل في مجال خدمة الجماعةفي عندنا أيضًا دور
+
+228
+00:18:43,530 --> 00:18:46,370
+المنظم الاجتماعي من وجهة نظر الـ Neustator اللي هي
+
+229
+00:18:46,370 --> 00:18:50,970
+العمليات الإدارية ركزلنا أيضًا على العمليات
+
+230
+00:18:50,970 --> 00:18:54,790
+الإدارية الدور اللي بقوم به اللي هو المنظم
+
+231
+00:18:54,790 --> 00:18:58,670
+الاجتماعي بعد العمل بين الجامعات اللي هي العمليات
+
+232
+00:18:58,670 --> 00:18:59,890
+الإدارية
+
+233
+00:19:01,780 --> 00:19:06,620
+العمليات الإدارية ووظائف الإدارة وغير ذلك فلذلك من
+
+234
+00:19:06,620 --> 00:19:10,080
+هنا لابد من إعداد البناء الملائم اللي بتنفيه
+
+235
+00:19:10,080 --> 00:19:13,400
+العمليات المهنية بين الجامعات والتأكد من وجود
+
+236
+00:19:13,400 --> 00:19:17,300
+لجيان الإداء الملائمة ووجود الإعداد الكافي من
+
+237
+00:19:17,300 --> 00:19:21,400
+الموظفين وتوزيع المسئوليات وتنظيم الإشراف على
+
+238
+00:19:21,400 --> 00:19:25,600
+النواحي المالية وتنظيم العلاقات العامة كل ذلك ما
+
+239
+00:19:25,600 --> 00:19:30,780
+يتعلق بوظائف الإدارة وظائف الإدارة تبتدرسوا إدارة
+
+240
+00:19:30,780 --> 00:19:36,000
+ولا لأ؟تمشي انتوا اللي بتدرسوا في الإدارة احنا
+
+241
+00:19:36,000 --> 00:19:39,840
+بندرسكوا تعاون سنة رابعة سنة رابعة احنا بندرسهم
+
+242
+00:19:39,840 --> 00:19:43,140
+إدارة فهذه كل اللي ذكرناها من وظائف الإدارة
+
+243
+00:19:43,140 --> 00:19:50,080
+التنسيق والإشراف والتخطيط والتوجيه والتمويل كل هذه
+
+244
+00:19:50,080 --> 00:19:54,720
+من وظائف الإدارة توزيع المسؤوليات والأدوار كل هذه
+
+245
+00:19:54,720 --> 00:19:59,050
+من وظائف الإدارةفلذلك من هنا ركّزنا على العمليات
+
+246
+00:19:59,050 --> 00:20:03,170
+الإدارية اللي ممكن يجون بيها المنظم الاجتماعي يعني
+
+247
+00:20:03,170 --> 00:20:07,930
+من خلال عملهم في المؤسسات الاجتماعية المختلفة في
+
+248
+00:20:07,930 --> 00:20:13,930
+المجتمعواضح؟ وأيضا التأكد أيضا من تشكيل اللجان
+
+249
+00:20:13,930 --> 00:20:19,090
+كأدوات من أدوات طريق التنظيم المجتمع وأيضا كتعتبر
+
+250
+00:20:19,090 --> 00:20:24,110
+بناء من البناء التكويني للمنظمة الاجتماعية إن
+
+251
+00:20:24,110 --> 00:20:29,390
+المنظمة الاجتماعية لها ثلاث مكونات أولا الجامعية
+
+252
+00:20:29,390 --> 00:20:35,150
+العمومية ثم مجلس الإدارة ثم اللجان واللجان هنا هي
+
+253
+00:20:35,150 --> 00:20:42,690
+الأداء الفاعلة من أجل تحقيق أهداف المؤسسةواضح؟ طيب
+
+254
+00:20:42,690 --> 00:20:48,890
+في عندنا أيضًا بالإضافة للعمل بين الجامعات و
+
+255
+00:20:48,890 --> 00:20:52,630
+القيام بالعمليات الإدارية أيضًا العمليات التربوية
+
+256
+00:20:52,630 --> 00:20:57,590
+العمليات التربوية فركزلنا على من وجهة نظر و
+
+257
+00:20:57,590 --> 00:21:01,570
+newsletter أيضًا على العمليات التربوية وهذه تتضمن
+
+258
+00:21:01,570 --> 00:21:07,270
+التدريب والتثقيف والتوعيةواضح سواء للعملين داخل
+
+259
+00:21:07,270 --> 00:21:10,890
+المؤسسة اللي هو جهاز تنظيم المجتمع اللي بنسميه
+
+260
+00:21:10,890 --> 00:21:15,250
+الهيئة الاجتماعية أو الجهاز أو في المجتمع ككل
+
+261
+00:21:15,250 --> 00:21:20,130
+لضمان التوافق بين فئات الفئات جميعا وهذا كله بيسر
+
+262
+00:21:20,130 --> 00:21:24,390
+لنا العمل المشترك بينها هذا كله عن طريق العمليات
+
+263
+00:21:24,390 --> 00:21:27,950
+التربوية، لذا هنا بنركز على اللي هو التثقيف
+
+264
+00:21:27,950 --> 00:21:34,370
+والتوعي، العمليات التثقيفية والتوعية للجمهورواضح؟
+
+265
+00:21:34,370 --> 00:21:41,850
+في طريق التنظيم المجتمعي أيضا
+
+266
+00:21:41,850 --> 00:21:46,030
+في عندنا عمليات أخرى اللي هي عمليات إنمائية أو
+
+267
+00:21:46,030 --> 00:21:52,300
+إنشائيةالعمليات الإنمائية والانشائية إذا فعلا أربع
+
+268
+00:21:52,300 --> 00:21:57,040
+عمليات العمل بين الجامعات من الأولى العمل الإداري
+
+269
+00:21:57,040 --> 00:22:02,440
+العمليات الإدارية العمليات التربوية والتثقيفية ثم
+
+270
+00:22:02,440 --> 00:22:06,900
+بعد ذلك العمليات الإنمائية والانشائية وهذه تقوم
+
+271
+00:22:06,900 --> 00:22:12,420
+بدور التنمية من أجل إحداث التغيير الاجتماعي داخل
+
+272
+00:22:12,420 --> 00:22:17,050
+المجتمعوفي فرق بين التغير والتغيير احداث تغيير
+
+273
+00:22:17,050 --> 00:22:20,990
+اجتماعي لأن التغيير هو مقصود تتدخل فيه الإرادة
+
+274
+00:22:20,990 --> 00:22:26,350
+الإنسانية تغيير مقصود من قبل الإنسان التغير قد
+
+275
+00:22:26,350 --> 00:22:30,330
+يكون احيانا تغير طبيعي وتلقائي لكن هنا في تنظيم
+
+276
+00:22:30,330 --> 00:22:34,470
+المجتمع نحن نقصد بالتغير اللي هو التغيير الاجتماعي
+
+277
+00:22:34,860 --> 00:22:38,480
+التغيير المقصود نعم إحداثه فهنا ما يقصد به ليه
+
+278
+00:22:38,480 --> 00:22:42,420
+عمليات إنمائية أو إنشائية اللي هي إحداث التغيير
+
+279
+00:22:42,420 --> 00:22:47,900
+المطلوب واضح و التنمية و العمل على إحداث التنمية
+
+280
+00:22:47,900 --> 00:22:51,640
+داخل المجتمع و التنمية هذه اللي بتسببنا اللي هي
+
+281
+00:22:51,640 --> 00:22:56,130
+التغيير الاجتماعي إلى الأفضل لأن التنمية دائماهي
+
+282
+00:22:56,130 --> 00:23:01,210
+إحداث تغييرات إلى الجوانب الاجتماعية قد تكون هذه
+
+283
+00:23:01,210 --> 00:23:05,790
+الجوانب يعني إيجابية أما التغير أحيانا ممكن التغير
+
+284
+00:23:05,790 --> 00:23:10,170
+التلقائي قد يكون إيجابي وقد يكون سلبي واضح لكن
+
+285
+00:23:10,170 --> 00:23:14,630
+التنمية نحن نقصد بها إحداث تغييرات مقصودة إحداث
+
+286
+00:23:14,630 --> 00:23:18,570
+تغييرات مقصودة جذرية داخل المجتمع وهذا ما يقصده
+
+287
+00:23:18,570 --> 00:23:21,950
+اللي هي العمليات الإنمائية وانشائية من أجل إحداث
+
+288
+00:23:21,950 --> 00:23:26,240
+تغييرات داخل المجتمع إلى الأفضلمن خلال التنمية
+
+289
+00:23:26,240 --> 00:23:32,340
+وبرامج التنمية داخل المجتمع تغيير نعم إحداث تغيير
+
+290
+00:23:32,340 --> 00:23:36,760
+نعم ما يخالف تغير لكن نقصد به نحن التغيير التغيير
+
+291
+00:23:36,760 --> 00:23:41,600
+إلى الأفضل اللي يبدأ عن طريق التخطيط المقصود العام
+
+292
+00:23:41,600 --> 00:23:47,760
+المقصود يعني التخطيط يقوم على التخطيط طيب
+
+293
+00:23:47,760 --> 00:23:51,460
+نعم خلصنا
+
+294
+00:23:54,220 --> 00:23:57,940
+يعني نكون بذلك يعني انتهينا من دور المنظم
+
+295
+00:23:57,940 --> 00:24:01,960
+الاجتماعي كما وضحوا لنا أنه استطار في العمليات
+
+296
+00:24:01,960 --> 00:24:05,640
+الأربعة اللي هي العمل بين الجامعات وهذا بيقوم على
+
+297
+00:24:05,640 --> 00:24:10,000
+الدور التنسيقي بين الجامعات وبين الهيئات والمؤسسات
+
+298
+00:24:10,000 --> 00:24:13,600
+الاجتماعية داخل المجتمع وأيضا بيقوم على الدور
+
+299
+00:24:20,200 --> 00:24:26,320
+العمل الإداري يقوم
+
+300
+00:24:26,320 --> 00:24:31,280
+على تشغيل وظائف الإدارة مثل التنسيق والتخطيط
+
+301
+00:24:31,280 --> 00:24:36,880
+وتوزيع المسئوليات وغير ذلك داخل المجتمع والاشراف
+
+302
+00:24:36,880 --> 00:24:42,420
+والتوجيه هذه كلها أيضا عمليات إدارية العملية
+
+303
+00:24:42,420 --> 00:24:47,600
+الثالثة هي العملية التثقيفيةالعملية اللي هي
+
+304
+00:24:47,600 --> 00:24:52,460
+التربوية التثقيفية التي تقوم على تثقيف الجمهور
+
+305
+00:24:52,460 --> 00:24:57,980
+والعملية الرابعة هي العملية الإنمائية التي تقوم
+
+306
+00:24:57,980 --> 00:25:02,020
+على اللي هو أحداث التنمية داخل المجتمع من أجل
+
+307
+00:25:02,020 --> 00:25:06,360
+التغيير الاجتماعي المقصود إلى الأفضل وإلى الأحسن
+
+308
+00:25:06,950 --> 00:25:13,090
+لكون في هذه المحاضرة قد يعني تحدثنا عن العوامل
+
+309
+00:25:13,090 --> 00:25:16,390
+اللي قدت الاختلاف الكتاب حول صياغ دور المنظم
+
+310
+00:25:16,390 --> 00:25:20,830
+الاجتماعي ثم تحدثنا على نموذج من النماذج الغربية
+
+311
+00:25:20,830 --> 00:25:26,150
+حول دور المنظم الاجتماعي وهو دور وجهة نظر الـ
+
+312
+00:25:26,150 --> 00:25:30,210
+Neostater إن شاء الله في المحاضرة القادمة نكمل
+
+313
+00:25:30,210 --> 00:25:37,550
+وجهة نظر علماء الغرب ثم ننتقل إلى وجهة نظرالعلماء
+
+314
+00:25:37,550 --> 00:25:41,610
+العرب حول وجه .. حول دور المنظم الاجتماعي في أي
+
+315
+00:25:41,610 --> 00:25:46,570
+سؤال الآن؟ طيب إلى أن نلتقي في المحاضرة القادمة لو
+
+316
+00:25:46,570 --> 00:25:50,630
+كنا من لكل تحية وتقدير والسلام عليكم ورحمة الله
+
+317
+00:25:50,630 --> 00:25:51,850
+وبركاته
+
diff --git a/PL9fwy3NUQKwZ8bh257eMcqZ22bPBOhO8z/jr9mXUSVWl4_raw.json b/PL9fwy3NUQKwZ8bh257eMcqZ22bPBOhO8z/jr9mXUSVWl4_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..985bccac6b62e6889d4175114de955c747747671
--- /dev/null
+++ b/PL9fwy3NUQKwZ8bh257eMcqZ22bPBOhO8z/jr9mXUSVWl4_raw.json
@@ -0,0 +1 @@
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0.9949544270833334}, {"start": 2123.75, "end": 2124.01, "word": " من", "probability": 0.95654296875}, {"start": 2124.01, "end": 2124.41, "word": " أدوات", "probability": 0.97314453125}, {"start": 2124.41, "end": 2124.79, "word": " تنظيم", "probability": 0.9945068359375}, {"start": 2124.79, "end": 2125.45, "word": " المجتمع", "probability": 0.994140625}], "temperature": 1.0}, {"id": 94, "seek": 213930, "start": 2126.08, "end": 2139.3, "text": "وإن شاء الله نكمل في المحاضرة أو في المحاضرات أو في المحاضرة القادمة وإلى أن نلتقي بكنا لكن مني كل تحية و تقدير والسلام عليكنا ورحمة الله وبركاته", "tokens": [2407, 28814, 1863, 13412, 16606, 21984, 8717, 24793, 1211, 8978, 9673, 5016, 46958, 25720, 34051, 8978, 9673, 5016, 46958, 2288, 9307, 34051, 8978, 9673, 5016, 46958, 25720, 25062, 18513, 46007, 4032, 28814, 23942, 14739, 8717, 1211, 2655, 38436, 4724, 4117, 8315, 5296, 19452, 9154, 1829, 28242, 6055, 5016, 10632, 4032, 6055, 28543, 13546, 16070, 3794, 37440, 25894, 4117, 8315, 4032, 2288, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224], "avg_logprob": -0.131361614380564, "compression_ratio": 1.8013698630136987, "no_speech_prob": 1.1920928955078125e-07, "words": [{"start": 2126.08, "end": 2126.62, "word": "وإن", "probability": 0.7744954427083334}, {"start": 2126.62, "end": 2126.82, "word": " شاء", "probability": 0.99169921875}, {"start": 2126.82, "end": 2127.14, "word": " الله", "probability": 0.96484375}, {"start": 2127.14, "end": 2127.62, "word": " نكمل", "probability": 0.9850260416666666}, {"start": 2127.62, "end": 2127.74, "word": " في", "probability": 0.962890625}, {"start": 2127.74, "end": 2128.44, "word": " المحاضرة", "probability": 0.9769287109375}, {"start": 2128.44, "end": 2128.96, "word": " أو", "probability": 0.66845703125}, {"start": 2128.96, "end": 2129.14, "word": " في", "probability": 0.94189453125}, {"start": 2129.14, "end": 2129.82, "word": " المحاضرات", "probability": 0.98876953125}, {"start": 2129.82, "end": 2130.02, "word": " أو", "probability": 0.875}, {"start": 2130.02, "end": 2130.16, "word": " في", "probability": 0.94580078125}, {"start": 2130.16, "end": 2130.62, "word": " المحاضرة", "probability": 0.9940185546875}, {"start": 2130.62, "end": 2131.24, "word": " القادمة", "probability": 0.9957682291666666}, {"start": 2131.24, "end": 2132.44, "word": " وإلى", "probability": 0.8400065104166666}, {"start": 2132.44, "end": 2132.6, "word": " أن", "probability": 0.91845703125}, {"start": 2132.6, "end": 2133.04, "word": " نلتقي", "probability": 0.9749755859375}, {"start": 2133.04, "end": 2133.6, "word": " بكنا", "probability": 0.7557779947916666}, {"start": 2133.6, "end": 2134.0, "word": " لكن", "probability": 0.580810546875}, {"start": 2134.0, "end": 2134.46, "word": " مني", "probability": 0.78759765625}, {"start": 2134.46, "end": 2134.68, "word": " كل", "probability": 0.85595703125}, {"start": 2134.68, "end": 2135.14, "word": " تحية", "probability": 0.9251302083333334}, {"start": 2135.14, "end": 2135.34, "word": " و", "probability": 0.90771484375}, {"start": 2135.34, "end": 2135.92, "word": " تقدير", "probability": 0.6940104166666666}, {"start": 2135.92, "end": 2136.74, "word": " والسلام", "probability": 0.794677734375}, {"start": 2136.74, "end": 2137.46, "word": " عليكنا", "probability": 0.7941080729166666}, {"start": 2137.46, "end": 2138.04, "word": " ورحمة", "probability": 0.99169921875}, {"start": 2138.04, "end": 2138.4, "word": " الله", "probability": 0.974609375}, {"start": 2138.4, "end": 2139.3, "word": " وبركاته", "probability": 0.9830078125}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 2139.9045, "duration_after_vad": 2069.8449999999957}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZ8bh257eMcqZ22bPBOhO8z/n9I3A_-fNPA.srt b/PL9fwy3NUQKwZ8bh257eMcqZ22bPBOhO8z/n9I3A_-fNPA.srt
new file mode 100644
index 0000000000000000000000000000000000000000..1b0b71a0f3afad9b2b1f83438f82bb0d0d65d346
--- /dev/null
+++ b/PL9fwy3NUQKwZ8bh257eMcqZ22bPBOhO8z/n9I3A_-fNPA.srt
@@ -0,0 +1,1263 @@
+1
+00:00:21,240 --> 00:00:26,060
+بسم الله الرحمن الرحيم الحمد لله رب العالمين
+
+2
+00:00:26,060 --> 00:00:30,280
+والصلاة والسلام على أشرف المرسلين سيدنا محمد وعلى
+
+3
+00:00:30,280 --> 00:00:35,480
+آله وصحبه أجمعين، بنات الطالبات، السلام عليكم ورحمة
+
+4
+00:00:35,480 --> 00:00:40,000
+الله وبركاته، كنا
+
+5
+00:00:40,000 --> 00:00:45,200
+في المحاضرة السابقة نتكلم عن تصنيفات أجهزة تنظيم المجتمع.
+
+6
+00:00:45,200 --> 00:00:51,290
+وتحدثنا عن الأجهزة الحكومية
+
+7
+00:00:51,290 --> 00:00:56,850
+والأجهزة الأهلية والأجهزة التي تجمع ما بين الاثنين،
+
+8
+00:00:56,850 --> 00:01:04,150
+الحكومية والأهلية. في هذه المحاضرة سنتحدث عن أهداف
+
+9
+00:01:04,150 --> 00:01:13,050
+أجهزة تنظيم المجتمع ومتطلباتها. طبعا بالنسبة لأجهزة
+
+10
+00:01:13,050 --> 00:01:19,550
+تنظيم المجتمع، أهدافها تنبثق من أهداف المجتمع، وأيضا
+
+11
+00:01:19,550 --> 00:01:26,710
+تنبثق من أهداف طريقة تنظيم المجتمع، فلابد
+
+12
+00:01:26,710 --> 00:01:32,470
+أن يكون هناك توافق ما بين أهداف الجهاز، جهاز تنظيم
+
+13
+00:01:32,470 --> 00:01:36,210
+المجتمع، وما بين أهداف الطريقة، أهداف طريقة
+
+14
+00:01:36,210 --> 00:01:41,470
+تنظيم المجتمع. وأيضا أهداف طريقة تنظيم المجتمع
+
+15
+00:01:41,470 --> 00:01:47,250
+أيضا هي نفسها أهداف مهنة الخدمة الاجتماعية. الأمور
+
+16
+00:01:47,250 --> 00:01:52,390
+هذه بدنا نعرفها. إذا أجهزة تنظيم المجتمع تنبثق من
+
+17
+00:01:52,390 --> 00:01:59,430
+طريقة تنظيم المجتمع وأهدافها، وأهداف طريقة تنظيم
+
+18
+00:01:59,430 --> 00:02:03,610
+المجتمع تنبثق من مهنة الخدمة الاجتماعية لأنها نفس
+
+19
+00:02:03,610 --> 00:02:08,250
+الأهداف، بينها لأنها طريقة من طرق الخدمة الاجتماعية،
+
+20
+00:02:08,250 --> 00:02:14,510
+طريقة تنظيم المجتمع. وأيضا الكل سواء الأجهزة أو
+
+21
+00:02:14,510 --> 00:02:19,770
+الطريقة أو المهنة، مهنة الخدمة الاجتماعية، كل ذلك
+
+22
+00:02:19,770 --> 00:02:25,150
+أهدافهم تتبثق من أين؟ من المجتمع، لذلك لابد أن يكون
+
+23
+00:02:25,150 --> 00:02:30,410
+هناك تطابق ما بين أهداف جهاز تنظيم المجتمع وأهداف
+
+24
+00:02:30,410 --> 00:02:34,750
+الطريقة ومهنة الخدمة الاجتماعية، وما بين أهداف
+
+25
+00:02:34,750 --> 00:02:39,570
+المجتمع، وأن لا يكون هناك تناقض بين هذه الأهداف،
+
+26
+00:02:39,570 --> 00:02:43,310
+أهداف واحدة، والكل يسعى إلى تحقيقها.
+
+27
+00:02:49,490 --> 00:02:52,670
+طبعا احنا في المؤسسات أو في أجهزة تنظيم المجتمع
+
+28
+00:02:52,670 --> 00:02:57,950
+نسعى إلى تحقيق أهداف المجتمع. عندما نضع الأهداف
+
+29
+00:02:57,950 --> 00:03:01,390
+الخاصة بالجهاز، لابد هذه الأهداف تكون منبثقة من
+
+30
+00:03:01,390 --> 00:03:06,170
+المجتمع، والأجهزة كلها، أجهزة طريقة تنظيم المجتمع
+
+31
+00:03:06,170 --> 00:03:13,850
+تسعى إلى تحقيق أهداف المجتمع. طيب،
+
+32
+00:03:13,850 --> 00:03:18,760
+ما هو الهدف العام لطريقة تنظيم المجتمع؟ مين تقول
+
+33
+00:03:18,760 --> 00:03:21,820
+ياخدناها في السنة الأولى، فيه أهداف، طريقة تنظيم
+
+34
+00:03:21,820 --> 00:03:29,160
+المجتمع، ما هو الهدف بالضبط؟ يعني احنا حددنا الهدف
+
+35
+00:03:29,160 --> 00:03:34,260
+العام لطريقة تنظيم المجتمع هو عبارة عن حل المشكلات
+
+36
+00:03:34,260 --> 00:03:39,980
+واشباع الحاجات، وممكن نقول أيضا عنه الهدف العام
+
+37
+00:03:39,980 --> 00:03:43,640
+لطريقة تنظيم المجتمع هو رفع مستوى معيشة المواطنين،
+
+38
+00:03:43,640 --> 00:03:46,860
+فهذا هو الهدف الذي تسعى إليه طريقة تنظيم المجتمع
+
+39
+00:03:46,860 --> 00:03:53,120
+وتسعى إليه أي مؤسسة من مؤسسات طريقة تنظيم
+
+40
+00:03:53,120 --> 00:03:53,700
+المجتمع.
+
+41
+00:03:56,430 --> 00:04:00,070
+نعم، ما هي التنمية؟ إلى ماذا تسعى؟ ما هي كلها في
+
+42
+00:04:00,070 --> 00:04:02,910
+إطار التنمية، ما هي طريقة تنظيم المجتمع تسعى
+
+43
+00:04:02,910 --> 00:04:06,410
+للتنمية، طب التنمية في إيش؟ في رفع مستوى معيشة
+
+44
+00:04:06,410 --> 00:04:10,890
+المواطنين، رفع مستوى معيشة المواطنين وحل مشكلاتهم
+
+45
+00:04:10,890 --> 00:04:15,530
+واشباع حاجاتهم. هذا هو الهدف العام لطريقة تنظيم
+
+46
+00:04:15,530 --> 00:04:21,960
+المجتمع. الآن نتكلم عن متطلبات الأهداف لطريقة تنظيم
+
+47
+00:04:21,960 --> 00:04:29,240
+المجتمع. طبعا لابد أن تتخذ في الاعتبار مجموعة من
+
+48
+00:04:29,240 --> 00:04:35,200
+المتطلبات لتتمكن الأجهزة، أجهزة تنظيم المجتمع، من تحقيق
+
+49
+00:04:35,200 --> 00:04:40,510
+أهدافها. احنا طبعا اخذنا الأهداف بشكل عام، في الفصل
+
+50
+00:04:40,510 --> 00:04:43,890
+الأول أو في الجزء الأول، لكن الآن بنتكلم عن
+
+51
+00:04:43,890 --> 00:04:47,570
+المتطلبات. تكلمنا عن الهدف العام لطريقة تنظيم
+
+52
+00:04:47,570 --> 00:04:52,670
+المجتمع، الآن بدنا، ما هي المتطلبات اللازمة لتحقيق
+
+53
+00:04:52,670 --> 00:04:57,170
+الأهداف؟ ما هي المتطلبات اللازمة لتحقيق الأهداف؟
+
+54
+00:04:57,860 --> 00:05:01,700
+نعم، بدي كل طالبة تقول لي متطلب من هذه المطالبات،
+
+55
+00:05:01,700 --> 00:05:07,420
+نعم يا بنتي، أيوة، عضوية
+
+56
+00:05:07,420 --> 00:05:12,560
+كافية، الحصول والإبقاء على عضوية كافية، والعمل
+
+57
+00:05:12,560 --> 00:05:19,140
+على اختيار أعضاء جدد، طبقا للمعايير التي تضمنها
+
+58
+00:05:19,140 --> 00:05:24,560
+اللائحة أو النظام، واضح؟ ماذا يا بنتي؟
+
+59
+00:05:27,660 --> 00:05:33,900
+التدريب أيضا، تدريب الأعضاء، التدريب مهم جدا، وفي
+
+60
+00:05:33,900 --> 00:05:35,340
+جهاز تنظيم المجتمع.
+
+61
+00:05:40,700 --> 00:05:44,920
+وهذا الشيء طبيعي، الذي هو تنمية التفاعل والاتصال ما
+
+62
+00:05:44,920 --> 00:05:50,040
+بين الأعضاء الموجودين في المنظمة، وما بين كل مكونات
+
+63
+00:05:50,040 --> 00:05:59,880
+المنظمة. طبعا
+
+64
+00:05:59,880 --> 00:06:03,560
+تقسيم العمل مهم جدا أيضا، هذه من المتطلبات من أجل
+
+65
+00:06:03,560 --> 00:06:07,120
+تحقيق الأهداف. لابد من تقسيم العمل وتوزيعه على
+
+66
+00:06:07,120 --> 00:06:08,960
+العاملين داخل المنظمة.
+
+67
+00:06:13,850 --> 00:06:18,050
+طبعا إسناد المسئوليات والأدوار للأعضاء داخل
+
+68
+00:06:18,050 --> 00:06:21,490
+المنظمة، حتى يعرف كل عضو من أعضاء المنظمة ما هو
+
+69
+00:06:21,490 --> 00:06:25,850
+المطلوب منه في عمله داخل المنظمة حتى يقوم بتحقيق
+
+70
+00:06:25,850 --> 00:06:29,630
+هذا العمل، وهذا مهم جدا، التي هي إسناد الأدوار
+
+71
+00:06:29,630 --> 00:06:32,630
+لأعضاء المنظمة. أيوة يا بنتي، في الآخر
+
+72
+00:06:37,030 --> 00:06:40,610
+تنظيم العلاقات بين مكونات المنظمة ككل، يعني بين
+
+73
+00:06:40,610 --> 00:06:46,010
+أقسامها المختلفة، لابد من تنظيم هذه العلاقات كلها
+
+74
+00:06:46,010 --> 00:06:50,770
+وإحداث تكامل ما بين أقسامها المختلفة، كله من أجل
+
+75
+00:06:50,770 --> 00:06:56,730
+ماذا؟ من أجل تحقيق أهداف المنظمة، ماذا أيضا؟ أيوة
+
+76
+00:06:56,730 --> 00:07:00,490
+بنتي، هناك؟ تنظيم العلاقات بين مكونات المنظمة،
+
+77
+00:07:00,490 --> 00:07:05,650
+نعم، تنظيم العلاقات وإيجاد تكامل ما بينها، نعم،
+
+78
+00:07:05,650 --> 00:07:11,250
+ماذا أيضا؟ العمل على أن يتبنى الأعضاء قيم اجتماعية
+
+79
+00:07:11,250 --> 00:07:13,970
+مشتركة، ومن ضمن هذه القيم الموافقة على أهداف
+
+80
+00:07:13,970 --> 00:07:17,330
+المنظمة والعمل من أجلها. طبعا احنا لما نعمل في أي
+
+81
+00:07:17,330 --> 00:07:21,670
+مؤسسة أو منظمة أو هيئة أو جهاز من أجهزة تنظيم
+
+82
+00:07:21,670 --> 00:07:26,690
+المجتمع، لابد أن نعرف ما هي أهداف هذه المنظمة أو
+
+83
+00:07:26,690 --> 00:07:32,050
+المؤسسة، ويكون كل موظف أو عامل داخل المنظمة يعلم
+
+84
+00:07:32,050 --> 00:07:36,710
+بهذه الأهداف، لماذا؟ حتى يعرف هذه الأهداف ويقوم
+
+85
+00:07:36,710 --> 00:07:40,430
+على تحقيقها، وما هو المطلوب منهم في تحقيق هذه
+
+86
+00:07:40,430 --> 00:07:44,470
+الأهداف. لذلك لابد من الإلمام بأهداف المنظمة عندما
+
+87
+00:07:44,470 --> 00:07:49,690
+نذهب للمؤسسة، نتدرب فيها أو نعمل فيها، لابد أن نعرف
+
+88
+00:07:49,690 --> 00:07:53,570
+ما هي هذه المؤسسة، وما طبيعة عملها، وما هي الأهداف
+
+89
+00:07:53,570 --> 00:07:57,150
+الخاصة بهذه المنظمة حتى نقوم نحن من خلال تدريبنا
+
+90
+00:07:57,150 --> 00:08:01,850
+أو عملنا على تحقيق هذه الأهداف. وهذا الشيء الذي أول
+
+91
+00:08:01,850 --> 00:08:06,710
+ما الموظف يذهب للمؤسسة، لابد يتعرف عليها وعلى
+
+92
+00:08:06,710 --> 00:08:10,110
+أهدافها بالدرجة الأولى، وما طبيعة الخدمات التي
+
+93
+00:08:10,110 --> 00:08:14,290
+تقدمها، ومن هم المستفيدون من خدماتها، هذا كله لابد
+
+94
+00:08:14,290 --> 00:08:19,010
+أن يلم به الموظف، وهذا متطلب من المتطلبات اللازمة
+
+95
+00:08:19,010 --> 00:08:24,610
+لتحقيق أهداف المنظمة. أيوة يا بنتي، العمل على تصور
+
+96
+00:08:24,610 --> 00:08:27,030
+المنظمة للموارد التي تحتاجها من البيئة
+
+97
+00:08:27,030 --> 00:08:32,410
+الاجتماعية، وهذا مهم جدا، المنظمة أحيانا تحتاج
+
+98
+00:08:32,410 --> 00:08:37,270
+لموارد، تحتاج لإمكانيات، فلابد أن تكون على علاقة
+
+99
+00:08:37,270 --> 00:08:40,770
+طيبة مع المجتمع الذي توجد فيه، لذلك من هنا
+
+100
+00:08:40,770 --> 00:08:45,390
+ميزانيتها، مواردها، إمكانياتها المادية والبشرية
+
+101
+00:08:45,390 --> 00:08:49,010
+تعتمد كثيرا على موارد المجتمع وعلى إمكانيات
+
+102
+00:08:49,010 --> 00:08:52,890
+المجتمع، ولابد أن تعلم المنظمة ما هي موارد المجتمع
+
+103
+00:08:53,260 --> 00:08:56,560
+وما هي الإمكانيات، وكيف تستفيد من هذه الموارد
+
+104
+00:08:56,560 --> 00:09:00,500
+والإمكانيات اللازمة للمجتمع، حتى تكون في العملية
+
+105
+00:09:00,500 --> 00:09:04,720
+الإيه؟ المدخلات لهذه المؤسسة، وبعد ذلك تخرج على شكل
+
+106
+00:09:04,720 --> 00:09:09,120
+مخرجات، حتى نقول هناك دورة مستمرة ما بين المدخلات
+
+107
+00:09:09,120 --> 00:09:13,900
+من موارد المجتمع، وأيضا المخرجات من خدمات المؤسسة
+
+108
+00:09:13,900 --> 00:09:19,160
+وبرامج المؤسسة ومشاريع للمجتمع. هذه كل ما يلزم من
+
+109
+00:09:19,160 --> 00:09:23,830
+متطلبات. ماذا أيضا؟ أيوة بنتي؟ إيجاد الوسائل اللازمة
+
+110
+00:09:23,830 --> 00:09:27,270
+لتنظيم عملية اتخاذ القرارات في المنظمة، الوسائل
+
+111
+00:09:27,270 --> 00:09:31,790
+اللازمة لتنظيم عملية اتخاذ القرارات، وطبعا مهم جدا
+
+112
+00:09:31,790 --> 00:09:35,610
+اتخاذ القرارات، أين هتتم؟ كيف؟ عن طريق لجان؟ عن طريق
+
+113
+00:09:35,610 --> 00:09:40,570
+مجلس إدارة؟ من هم الذين يقومون باتخاذ القرارات
+
+114
+00:09:40,570 --> 00:09:44,930
+داخل المؤسسة؟ هذه مهم جدا، اتخاذ القرارات، الأشخاص
+
+115
+00:09:44,930 --> 00:09:48,650
+الذين يتخذون القرارات، ومسؤولياتهم ووظائفهم داخل
+
+116
+00:09:48,650 --> 00:09:54,180
+المؤسسة، من هم الذين يتخذون القرارات؟ واضح؟ هذه كلها
+
+117
+00:09:54,180 --> 00:09:59,320
+لابد أن يعني تكون معروفة، حتى تستطيع المؤسسة أن
+
+118
+00:09:59,320 --> 00:10:03,160
+تحقق أهدافها، لأن بدون معرفة هذه الأشياء لا يمكن أن
+
+119
+00:10:03,160 --> 00:10:07,740
+تحقق المؤسسة أهدافها. أيوة يا بنتي، في الآخر إيش
+
+120
+00:10:07,740 --> 00:10:15,080
+كمان فيه بعد ذلك؟ تنسيق بين الأنشطة التنظيمية بحسب
+
+121
+00:10:15,080 --> 00:10:19,840
+سِعة التنفيذ، على الأنشطة التنظيمية. نعم، التنسيق
+
+122
+00:10:19,840 --> 00:10:24,240
+بينها، وطبعا تنسيق بين أعمال المنظمة وأنشطتها
+
+123
+00:10:24,240 --> 00:10:27,860
+المختلفة مهم جدا، لابد أن ننسق بين أعمالها وبين
+
+124
+00:10:27,860 --> 00:10:32,800
+أنشطتها المختلفة حتى يمكننا تحقيق الأهداف الخاصة
+
+125
+00:10:32,800 --> 00:10:37,760
+بالمنظمة، لأن بدون التنسيق الأمور بتكون متشعبة، و
+
+126
+00:10:37,760 --> 00:10:42,140
+تحدث فوضى داخل المؤسسة، ولا يمكن إنجاز أي شيء من
+
+127
+00:10:42,140 --> 00:10:46,420
+الأعمال. لذلك التنسيق مهم جدا في البرامج والخدمات
+
+128
+00:10:46,420 --> 00:10:49,420
+وفي الأنشطة التي تقدمها هذه المؤسسة، كله يعني
+
+129
+00:10:49,420 --> 00:10:53,200
+التنسيق بين الأفراد كذلك، والأعمال المطلوبة من كل
+
+130
+00:10:53,200 --> 00:10:56,920
+فرد داخل المنظمة، والأقسام الخاصة بالمنظمة، هذه
+
+131
+00:10:56,920 --> 00:11:00,960
+كلها لابد من التنسيق بينها حتى نستطيع نوفر الوقت
+
+132
+00:11:01,260 --> 00:11:06,000
+والجهد والتكاليف، وأن لا تحدث ازدواجية في أداء
+
+133
+00:11:06,000 --> 00:11:12,760
+الأعمال، لأن عدم التنسيق قد يؤدي إلى الازدواجية،
+
+134
+00:11:12,760 --> 00:11:17,440
+كيف تكون هذه الازدواجية؟ ممكن موظف يقوم بعمل، وموظف
+
+135
+00:11:17,440 --> 00:11:21,180
+آخر يقوم بنفس العمل، طب لماذا؟ إذن هنا لابد من
+
+136
+00:11:21,180 --> 00:11:25,200
+التنسيق. أو قسم يقوم بتحقيق أهداف معينة، وقسم آخر
+
+137
+00:11:25,200 --> 00:11:28,560
+يقوم بنفس تحقيق الأهداف، طب لماذا؟ إذن هنا لابد من
+
+138
+00:11:28,560 --> 00:11:32,500
+التنسيق بين الأقسام المختلفة داخل المؤسسة حتى لا
+
+139
+00:11:32,500 --> 00:11:37,640
+تحدث الازدواجية في تحقيق الأهداف، ونوفر ما قلنا من
+
+140
+00:11:37,640 --> 00:11:42,420
+وقت وجهد وتكاليف. ماذا أيضا؟ أيوة بنتي
+
+141
+00:11:45,410 --> 00:11:50,430
+مهمة جدا هذه، هذه مهمة جدا أيضا، لأن كيف أنا أطلب
+
+142
+00:11:50,430 --> 00:11:54,470
+من أفراد المنظمة أو جهاز تنظيم المجتمع أن يحقق
+
+143
+00:11:54,470 --> 00:11:59,050
+أهداف الجهاز، ولا يستطيع الجهاز أن يحقق أهدافه،
+
+144
+00:11:59,050 --> 00:12:04,870
+والعاملين فيه. لذلك من هنا لابد أن نهتم بمشكلات
+
+145
+00:12:04,870 --> 00:12:10,770
+العاملين، ومعرفة حاجاتهم، وما هي متطلباتهم، ويقوم أو
+
+146
+00:12:10,770 --> 00:12:16,170
+تقوم هذه المؤسسة أو هذه المنظمة على إشباع هذه
+
+147
+00:12:16,170 --> 00:12:21,170
+الحاجات، وحل المشكلات الخاصة بالعاملين داخل المنظمة.
+
+148
+00:12:21,170 --> 00:12:26,920
+وإذا لم نهتم، الأمور يعني قد تتفاقم وقد ينتج عنها
+
+149
+00:12:26,920 --> 00:12:32,360
+أشياء سلبية. لذلك المؤسسة الناجحة هي التي تُحسب
+
+150
+00:12:32,360 --> 00:12:37,580
+لعاملين لديها، هي التي تحس بمتطلبات العاملين لديها،
+
+151
+00:12:37,580 --> 00:12:42,700
+وتقوم على الاستماع لهم، للعاملين جميعا داخل هذه
+
+152
+00:12:42,700 --> 00:12:47,760
+المنظمة، ومعرفة مشكلاتهم واحتياجاتهم، وتقوم هذه
+
+153
+00:12:47,760 --> 00:12:51,500
+المنظمة بحل هذه المشكلات، وإشباع الاحتياجات الخاصة
+
+154
+00:12:51,500 --> 00:12:56,300
+بالعاملين، حتى يستطيع العاملون داخل المؤسسة أو
+
+155
+00:12:56,300 --> 00:13:01,120
+المنظمة القيام بواجباتهم على أكمل وجه ممكن، وتحقيق
+
+156
+00:13:01,120 --> 00:13:06,220
+أهداف المنظمة. لذلك من هنا، هذه المتطلبات المادية
+
+157
+00:13:06,220 --> 00:13:11,340
+والمعنوية مهمة جدا، أن نتعرف عليها لدى العاملين
+
+158
+00:13:11,340 --> 00:13:16,900
+والمسؤولين لدى المنظمة، أن يقوموا بحل هذه المشكلات
+
+159
+00:13:16,900 --> 00:13:21,410
+إذا كانت توجد مشكلات، وإشباع الحاجات، إذا كانت توجد
+
+160
+00:13:21,410 --> 00:13:25,610
+لهم حاجات أيضا، والاستماع والإنصات لهم جيدا،
+
+161
+00:13:25,610 --> 00:13:29,650
+والتفاهم معهم جيدا، مع العاملين، وأن يكون هناك
+
+162
+00:13:29,650 --> 00:13:34,330
+تقارب ما بين الإدارة والعمال داخل المنظمة، حتى
+
+163
+00:13:34,330 --> 00:13:38,230
+تستطيع المنظمة الوصول إلى تحقيق أهدافها بأعلى
+
+164
+00:13:38,230 --> 00:13:43,810
+كفاءة، ويعني تكون أيضا حققت المطلوب من أهدافها
+
+165
+00:13:43,810 --> 00:13:50,860
+بشكل ممتاز داخل المجتمع. لذلك الاهتمام بالعمل
+
+166
+00:13:50,860 --> 00:13:55,480
+أهم من الاهتمام بالمبنى. المبنى شيء مادي، ممكن أبنية
+
+167
+00:13:55,480 --> 00:13:59,720
+في ستة شهور، مدرسة ممكن أبنيها في ستة شهور، ولكن
+
+168
+00:13:59,720 --> 00:14:05,900
+المعلم .. كم يحتاج من وقت حتى أبني المعلم؟ أربعة
+
+169
+00:14:05,900 --> 00:14:11,280
+سنين، مين بتقول كم سنة؟ يحتاج المعلم، المعلم كم
+
+170
+00:14:11,280 --> 00:14:17,800
+سنة؟ أيوة، أحسنتي، المعلم يحتاج منذ ولادته، منذ أن
+
+171
+00:14:17,800 --> 00:14:22,310
+يولد، لابد نعتني به وهو في الروضة، فانت كطفل، ثم
+
+172
+00:14:22,310 --> 00:14:25,930
+اعتني به في مرحلة الإبتدائية، ثم الإعدادية، ثم
+
+173
+00:14:25,930 --> 00:14:31,370
+الثانوية، ثم الجامعة، لكي يصبح معلما لمرحلة في
+
+174
+00:14:31,370 --> 00:14:34,490
+التعليم العام، سواء كان في مرحلة الإبتدائية أو
+
+175
+00:14:34,490 --> 00:14:39,530
+الإعدادية أو الثانوية، كم سنة؟ 22 سنة هذه للمعلم
+
+223
+00:18:28,100 --> 00:18:31,620
+يكون هناك توافق ما بين أهدافها وأهداف المجتمع الذي
+
+224
+00:18:31,620 --> 00:18:36,820
+توجد فيه، لابد أن يكون هناك توافق، فلذلك المنظمة
+
+225
+00:18:36,820 --> 00:18:42,560
+الناجحة هي التي تنظر وترى وتتفحص وتعرف ما هي
+
+226
+00:18:42,560 --> 00:18:48,190
+المشكلات الخارجية وتحاول أن تتغلب عليها، ما هو الخطر
+
+227
+00:18:48,190 --> 00:18:54,470
+الخارجي وتحاول أن تتغلب عليه، واضح؟ وكذلك في داخل
+
+228
+00:18:54,470 --> 00:18:58,830
+المنظمة، تعرف من هم في صف المنظمة ومن هم خارج
+
+229
+00:18:58,830 --> 00:19:03,470
+المنظمة، هذه هي المؤسسة، ولابد من أجل تحقيق
+
+230
+00:19:03,470 --> 00:19:08,570
+الأهداف أن نعرف ما هو مع ومن هو ضد، حتى نستطيع أن
+
+231
+00:19:08,570 --> 00:19:13,310
+نسير بأهداف المنظمة على خير ما يرام، طبق الأهداف
+
+232
+00:19:13,310 --> 00:19:19,840
+المجتمعة، هذه أمور أيضًا من المتطلبات التي هي تحقيق
+
+233
+00:19:19,840 --> 00:19:23,200
+أهداف المنظمة، وحمايتها من الأخطار الخارجية
+
+234
+00:19:23,200 --> 00:19:30,440
+والداخلية، واضح؟ وأحيانًا مؤسساتنا ومنظماتنا تكون
+
+235
+00:19:30,440 --> 00:19:35,580
+معرضة لأخطار خارجية، من أعداء، من أعداء أيضًا لنا،
+
+236
+00:19:35,580 --> 00:19:41,120
+لذلك لابد أن نتفهم هذه الأخطار، ونُحِيط المؤسسة أو
+
+237
+00:19:41,120 --> 00:19:42,620
+المنظمة من هذه الأخطار،
+
+238
+00:19:48,340 --> 00:19:53,540
+ماذا أيضًا من متطلبات لازمة للمنظمة؟ ما تكلمناش
+
+239
+00:19:53,540 --> 00:19:57,620
+بالمرة، أيه يا بنتي؟ إيجاد قوانين، لوائح، ونظام
+
+240
+00:19:57,620 --> 00:20:03,800
+قوانين المؤسسة طبعًا،
+
+241
+00:20:03,800 --> 00:20:10,040
+الذي هو الضبط، يعني القوانين، إيجاد قوانين، لوائح،
+
+242
+00:20:10,040 --> 00:20:18,360
+ونظام، قوانين المؤسسة، ضبط، حتى نضمن، حتى نضمن سير
+
+243
+00:20:18,360 --> 00:20:22,960
+المنظمة على خير ما يرام، وأن هناك ضوابط وقوانين
+
+244
+00:20:22,960 --> 00:20:28,840
+ونظم ولوائح تنظم عمل المنظمة، فلذلك من هنا لابد من
+
+245
+00:20:28,840 --> 00:20:33,420
+إيجاد هذه النظم والقوانين واللوائح الخاصة بالمنظمة،
+
+246
+00:20:36,510 --> 00:20:39,350
+وهذا ما نسميه الضبط الاجتماعي، أحيانًا الضبط
+
+247
+00:20:39,350 --> 00:20:43,990
+الاجتماعي ليس قوانين ونظم ولوائح رسمية، أحيانًا قد
+
+248
+00:20:43,990 --> 00:20:48,150
+يكون عادات وتقاليد وأعراف اعتادوا عليها العاملين
+
+249
+00:20:48,150 --> 00:20:53,490
+داخل المؤسسة، لابد أن تكون هذه العادات والتقاليد
+
+250
+00:20:53,490 --> 00:20:58,770
+منظمة أيضًا، ولا تتعارض مع قوانين المنظمة، يسير
+
+251
+00:20:58,770 --> 00:21:03,220
+عليها العاملون داخل المنظمة، فالضبط يشمل الناحيتين
+
+252
+00:21:03,220 --> 00:21:07,180
+الرسمية وغير الرسمية، وأحيانًا قد تنشأ أيضًا جامعات
+
+253
+00:21:07,180 --> 00:21:10,860
+غير رسمية، أو علاقات غير رسمية داخل المنظمة، هذه
+
+254
+00:21:10,860 --> 00:21:15,600
+لابد أن نستغلها ونستثمرها لصالح المنظمة، وهذه كانت
+
+255
+00:21:15,600 --> 00:21:18,460
+في التنظيم غير الرسمي، لأن أحيانًا يوجد تنظيم رسمي
+
+256
+00:21:18,460 --> 00:21:22,880
+في المؤسسة، وتنظيم غير رسمي ينشأ… من تقول لي ما
+
+257
+00:21:22,880 --> 00:21:26,820
+هو التنظيم الرسمي والتنظيم غير الرسمي؟ ما درستوش؟
+
+258
+00:21:26,820 --> 00:21:33,060
+… أيوة، تفضلي يا بنتي، المؤسسة ولوائح المنظمة، الذي هو
+
+259
+00:21:33,060 --> 00:21:39,300
+نفس العدد العالي؟ لا، هو التنظيم الرسمي هو الذي
+
+260
+00:21:39,300 --> 00:21:43,800
+ينشأ داخل المنظمة طبقًا للقوانين، يعني الموظفون يتم
+
+261
+00:21:43,800 --> 00:21:49,060
+اختيارهم حسب ماذا داخل المؤسسة؟ حسب القوانين والنظم
+
+262
+00:21:49,060 --> 00:21:53,100
+واللوائح التي تنظم حاجة المؤسسة للموظف داخل المنظمة،
+
+263
+00:21:53,560 --> 00:21:58,100
+لكن أحيانًا تنظيمات غير رسمية تنشأ داخل التنظيم
+
+264
+00:21:58,100 --> 00:22:01,220
+الرسمي، يعني بين العاملين الذين تم تعيينهم في
+
+265
+00:22:01,220 --> 00:22:04,720
+المؤسسة، نتيجة ماذا؟ نتيجة العلاقات الاجتماعية
+
+266
+00:22:04,720 --> 00:22:08,940
+التي تحدث بين العاملين، تنشأ هناك جماعات، نسميها
+
+267
+00:22:08,940 --> 00:22:15,540
+جماعات غير رسمية، تنشأ يعني داخل المؤسسة، هذه لابد
+
+268
+00:22:15,540 --> 00:22:19,320
+على الإدارة أن تستثمر هذه الجماعات غير الرسمية
+
+269
+00:22:19,320 --> 00:22:25,600
+لصالح العمل، لصالح عمل المنظمة، وتحقيق أهداف المنظمة،
+
+270
+00:22:25,600 --> 00:22:30,340
+وإلا تكون هذه الجماعات غير الرسمية لها انحرافات
+
+271
+00:22:30,340 --> 00:22:34,480
+سلوكية، انحرافات قد تؤدي إلى تعطيل الأعمال، لأن في
+
+272
+00:22:34,480 --> 00:22:37,580
+حالة لو كانت هذه الجماعات تؤدي إلى تعطيل الأعمال،
+
+273
+00:22:37,580 --> 00:22:41,060
+لابد من وقفها، ولكن إذا كانت هذه الجماعات التي تنشأ
+
+274
+00:22:41,060 --> 00:22:45,380
+نتيجة العلاقات الاجتماعية تساعد وتساهم في إنجاز
+
+275
+00:22:45,380 --> 00:22:50,020
+العمل، وتحقيق أهداف المنظمة على خير ما يرام، علينا
+
+276
+00:22:50,020 --> 00:22:53,560
+أن نشجع هذه الجماعات غير الرسمية داخل التنظيم
+
+277
+00:22:53,560 --> 00:22:57,700
+الرسمي، أو داخل الجماعة الرسمية في المؤسسة، حتى
+
+278
+00:22:57,700 --> 00:23:03,980
+نستطيع أن نحقق أهداف المؤسسة على خير ما يرام، طيب،
+
+279
+00:23:03,980 --> 00:23:07,820
+في عندنا أيضًا، ما زلنا في المتطلبات، أيوة يا بنتي،
+
+280
+00:23:07,820 --> 00:23:13,920
+نعم،
+
+281
+00:23:13,920 --> 00:23:17,780
+طبعًا،
+
+282
+00:23:17,780 --> 00:23:21,680
+هذه الوسائل التي تعالج الصراعات، وهذه مهمة جدًا، نعم،
+
+283
+00:23:21,680 --> 00:23:26,880
+إحنا ما نبيش… اشرحي هذه الوسائل، طيب كيف ما نبيش نجيب
+
+284
+00:23:26,880 --> 00:23:29,720
+الوسائل التي تُقَدِّر على الصراعات، يا بنتي، يلا، المهم
+
+285
+00:23:29,720 --> 00:23:32,620
+إحنا نفهم من البنت كيف ما نبيش نقدر على الصراعات،
+
+286
+00:23:39,680 --> 00:23:42,280
+نعم، وسائل هذه، برضه من ضمن الوسائل،
+
+287
+00:23:45,190 --> 00:23:48,710
+نعم، نعم، ماذا يا بنتي؟ ممكن لو كان في أكثر من
+
+288
+00:23:48,710 --> 00:23:53,950
+عامل أو موظف يتنافسون على منصب معين من خلال
+
+289
+00:23:53,950 --> 00:23:57,590
+انتخابات مثلًا في المؤسسة، ممكن، ممكن، وسيلة من
+
+290
+00:23:57,590 --> 00:24:02,030
+الوسائل، ممكن، هاه، هذه كلها يعني أننا نستطيع أن
+
+291
+00:24:02,030 --> 00:24:05,350
+نبتكر، ماذا أيضًا؟ من تقول لي أيضًا ماذا من وسائل
+
+292
+00:24:05,350 --> 00:24:08,370
+ممكن نستخدمها في القضاء على الصراعات بين العاملين
+
+293
+00:24:08,370 --> 00:24:11,800
+داخل المؤسسة من أجل تحقيق أهداف المؤسسة؟ قامت
+
+294
+00:24:11,800 --> 00:24:17,340
+لقاءات ومناقشات وحوارات، احتفظت… لقاءات ومناقشات،
+
+295
+00:24:17,340 --> 00:24:21,700
+براڤو، أحسنت، من الوسائل، ماذا أيضًا؟ حوارات والأشياء
+
+296
+00:24:21,700 --> 00:24:25,100
+بالعمل الجماعي، بالعمل الجماعي، تشجيع من العمل
+
+297
+00:24:25,100 --> 00:24:28,560
+الفردي للعمل الجماعي، برضه هادِم نقضي على الصراعات،
+
+298
+00:24:28,560 --> 00:24:35,040
+نعم، براڤو، الأنشطة مهمة جدًا، الأنشطة الاجتماعية، حفلات
+
+299
+00:24:35,040 --> 00:24:40,900
+ورحلات وزيارات، هذه كلها تساعد على الحد من الصراعات
+
+300
+00:24:40,900 --> 00:24:46,020
+داخل المؤسسة، وتعمل على إيجاد التعاون
+
+301
+00:24:46,020 --> 00:24:51,040
+والترابط والمحبة بين العاملين داخل المنظمة، هذه كلها
+
+302
+00:24:51,040 --> 00:24:54,240
+نستطيع بها القضاء على الصراعات، فلذلك من أجل تحقيق هذا
+
+303
+00:24:54,240 --> 00:24:57,600
+في المؤسسة أو المنظمة لابد أن نقضي على الصراعات
+
+304
+00:24:57,600 --> 00:25:01,860
+الداخلية، داخل… بين العاملين، داخل المنظمة، حتى
+
+305
+00:25:01,860 --> 00:25:06,420
+الجميع يتعاون من أجل تحقيق أهداف المنظمة، ماذا
+
+306
+00:25:06,420 --> 00:25:15,140
+أيضًا؟ من لم تتكلم نهائيًا؟ أيه، ومن منكن؟ وحدة
+
+307
+00:25:15,140 --> 00:25:20,180
+المنظمات وتكاملها، العمل على تنمية وحدة المنظمات،
+
+308
+00:25:20,180 --> 00:25:24,780
+طبعًا، التي هي العمل دائمًا على أن المنظمة، نخليها في
+
+309
+00:25:24,780 --> 00:25:29,680
+وحدة متكاملة، ما تكون هناك أقسام مفرقة، أو وحدات
+
+310
+00:25:29,680 --> 00:25:35,260
+مفرقة، كلها تكمل بعضها أيضًا، أن يكون هناك كل قسم
+
+311
+00:25:35,260 --> 00:25:40,820
+أو كل وحدة داخل المنظمة تقوم بعمل يكمل العمل الآخر
+
+312
+00:25:40,820 --> 00:25:46,620
+مع الوحدة الأخرى، وهذا من المتطلبات اللازمة لتحقيق
+
+313
+00:25:46,620 --> 00:25:50,760
+أهداف المؤسسة، حتى يكون هناك تكامل بين أقسامها
+
+314
+00:25:50,760 --> 00:25:55,720
+ووحداتها المختلفة، وإلا يكون كل قسم أو وحدة منفصلة
+
+315
+00:25:55,720 --> 00:26:01,200
+عن الأخرى، لابد الكل يتفهم ما هو المطلوب منه داخل
+
+316
+00:26:01,200 --> 00:26:04,860
+هذه المؤسسة من الوحدات والأقسام المختلفة، حتى
+
+317
+00:26:04,860 --> 00:26:08,800
+الجميع يعمل على تحقيق الأهداف العامة للمؤسسة أو
+
+318
+00:26:08,800 --> 00:26:11,700
+المنظمة، ماذا أيضًا؟ أيوة يا بنتي،
+
+319
+00:26:18,240 --> 00:26:24,840
+هذا مهم جدًا، الـ events أيضًا، طبعًا، طبعًا، طبعًا، هذه مهمة
+
+320
+00:26:24,840 --> 00:26:30,160
+جدا، الوسائل التي  ممكن نُغيِّر بها أهداف المنظمة، من بناء
+
+321
+00:26:30,160 --> 00:26:35,720
+المنظمة، نغير أيضًا في الموارد كذلك، نعمل على
+
+322
+00:26:35,720 --> 00:26:40,140
+زيادة موارد المنظمة، كيف نبدأ نعمل تنميتها،
+
+323
+00:26:40,140 --> 00:26:44,120
+واستثمارها في أشياء مفيدة، ربما هذه المنظمة أحيانًا
+
+324
+00:26:44,120 --> 00:26:49,560
+تعتمد على المساعدات والإعانات، فمن أجل تحقيق يمكن
+
+325
+00:26:49,560 --> 00:26:53,320
+أن تتوقف هذه المساعدات والإعانات من الجهة المانحة، أو من
+
+326
+00:26:53,320 --> 00:26:58,900
+الجهة التي تقدم هذه المساعدة أو المعونة، طيب هل تتوقف
+
+327
+00:26:58,900 --> 00:27:03,680
+المؤسسة أو المنظمة يعني من أجل إيقاف هذه المساعدات
+
+328
+00:27:03,680 --> 00:27:08,440
+والمعونات؟ لا طبعًا، هنا لابد من التفكير في كيفية
+
+329
+00:27:08,440 --> 00:27:14,580
+تنمية مواردها، في كيفية إيجاد مشاريع تنمي مواردها،
+
+330
+00:27:14,580 --> 00:27:19,740
+حتى تستطيع أن تنفق من هذه الموارد على من تقوم
+
+331
+00:27:19,740 --> 00:27:23,680
+بخدمتها، يعني مثلًا لو كانت جمعية تعمل في رعاية
+
+332
+00:27:23,680 --> 00:27:28,900
+المعاقين، وهذه الجمعية تحتاج إلى مساعدات وإعانات،
+
+333
+00:27:28,900 --> 00:27:32,880
+وقامت هذه الجمعية وأخذت هذه المساعدات والإعانات،
+
+334
+00:27:32,880 --> 00:27:38,040
+لكن الجهة التي تمنح أو تعطي هذه المساعدات يعني
+
+335
+00:27:38,040 --> 00:27:41,920
+سواء كانت حكومة أو كانت جهة من الخارج أو من الداخل
+
+336
+00:27:41,920 --> 00:27:46,060
+تمنح هذه المؤسسة الخاصة بالمعاقين، من هل تتوقف هذه
+
+337
+00:27:46,060 --> 00:27:50,780
+المؤسسة عن القيام بعملها الإنساني في رعاية المعاقين
+
+338
+00:27:50,780 --> 00:27:55,670
+وتقديم الخدمات اللازمة لهم؟ طبعًا، لابد من إيجاد و
+
+339
+00:27:55,670 --> 00:28:00,370
+تفكير في مشاريع استثمارية، مشاريع استثمارية تعود
+
+340
+00:28:00,370 --> 00:28:04,550
+بالفائدة على هذه المؤسسة حتى تنفق على المعاقين
+
+341
+00:28:05,490 --> 00:28:09,190
+الرعاية الاجتماعية، حتى تقوم برعايتهم، فممكن تعمل أي
+
+342
+00:28:09,190 --> 00:28:13,870
+مورد اقتصادي لتنمية هذه الموارد، ممكن تعمل مشروع
+
+343
+00:28:13,870 --> 00:28:18,530
+للأطعمة، زي إرادة مثلًا، عندها زي مشروع إرادة يعني
+
+344
+00:28:18,530 --> 00:28:24,290
+مشاريع في بناء، عمل أطعمة، عمل معجنات، عمل يعني إذا
+
+345
+00:28:24,290 --> 00:28:28,130
+كان لديها قطعة أرض، أن تستثمرها في الزراعة، وهكذا،
+
+346
+00:28:28,300 --> 00:28:31,460
+إذا هنا لابد من التفكير في المشاريع الزراعية،
+
+347
+00:28:31,460 --> 00:28:35,860
+الصناعية، التجارية، لماذا؟ حتى الدخل الذي يعود
+
+348
+00:28:35,860 --> 00:28:39,440
+والفائدة التي تعود على المؤسسة، تنفقها على رعاية
+
+349
+00:28:39,440 --> 00:28:44,360
+هذه الفئة المحتاجة للرعاية، وهي رعاية المعاقين داخل
+
+350
+00:28:44,360 --> 00:28:48,960
+مجتمعنا، وهكذا كل مؤسسة… المؤسسة الناجحة هي التي
+
+351
+00:28:48,960 --> 00:28:53,820
+تعتمد على ذاتها وعلى مواردها الذاتية، ولا تعتمد على
+
+352
+00:28:53,820 --> 00:28:58,880
+موارد خارجية فقط، هذه المؤسسة الناجحة التي
+
+353
+00:28:58,880 --> 00:29:03,900
+تستطيع أن تواجه الصعاب، وتستطيع أن تستمر في عطائها
+
+354
+00:29:03,900 --> 00:29:09,440
+المستمر، ولذلك من هنا نقول لابد من التي هي إيجاد
+
+355
+00:29:09,440 --> 00:29:14,160
+الوسائل اللازمة لتنمية موارد مؤسساتنا حتى تستطيع أن
+
+356
+00:29:14,160 --> 00:29:19,300
+تحقق أهدافها، إلى هنا يعني في أي سؤال في الأهداف
+
+357
+00:29:19,300 --> 00:29:22,580
+والمتطلبات، الهدف العام لطريقة تنظيم المجتمع،
+
+358
+00:29:22,580 --> 00:29:26,640
+والمتطلبات اللازمة لتحقيق أهداف طريقة تنظيم
+
+359
+00:29:26,640 --> 00:29:31,160
+المجتمع بشكل عام، طيب إلى هنا ننهي هذه المحاضرة على
+
+360
+00:29:31,160 --> 00:29:34,620
+أمل اللقاء بكم في المحاضرة القادمة بإذن الله،
+
+361
+00:29:34,620 --> 00:29:40,140
+تعالَ حول بناء أجهزة تنظيم المجتمع، إلى أن نلتقي في
+
+362
+00:29:40,140 --> 00:29:44,560
+المحاضرة القادمة لكم من كل تقدير واحترام والسلام
+
+363
+00:29:44,560 --> 00:29:46,840
+عليكم ورحمة الله وبركاته
diff --git a/PL9fwy3NUQKwZ8bh257eMcqZ22bPBOhO8z/n9I3A_-fNPA_raw.srt b/PL9fwy3NUQKwZ8bh257eMcqZ22bPBOhO8z/n9I3A_-fNPA_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..7047c9b21ef13196096333b40cf1349f7b62a4c3
--- /dev/null
+++ b/PL9fwy3NUQKwZ8bh257eMcqZ22bPBOhO8z/n9I3A_-fNPA_raw.srt
@@ -0,0 +1,1452 @@
+1
+00:00:21,240 --> 00:00:26,060
+بسم الله الرحمن الرحيم الحمد لله رب العالمين
+
+2
+00:00:26,060 --> 00:00:30,280
+والصلاة والسلام على أشرف المرسلين سيدنا محمد وعلى
+
+3
+00:00:30,280 --> 00:00:35,480
+آله وصحبه أجمعين بنات الطالبات السلام عليكم ورحمة
+
+4
+00:00:35,480 --> 00:00:40,000
+الله وبركاته كنا
+
+5
+00:00:40,000 --> 00:00:45,200
+في المحاضرة السابقة نتكلم عن تصنيفات أجهزة طريق
+
+6
+00:00:45,200 --> 00:00:51,290
+التنظيم المجتمعوتحدثنا عن الأجهزة الحكومية
+
+7
+00:00:51,290 --> 00:00:56,850
+والأجهزة الأهلية والأجهزة التي تجمع ما بين الإثنين
+
+8
+00:00:56,850 --> 00:01:04,150
+الحكومية والأهلية في هذه المحاضرة سنتحدث عن أهداف
+
+9
+00:01:04,150 --> 00:01:13,050
+أجهزة تنظيم المجتمع ومتطلباتها طبعا بالنسبة أجهزة
+
+10
+00:01:13,050 --> 00:01:19,550
+تنظيم المجتمع أهدافها تنبثق من أهداف المجتمعوأيضًا
+
+11
+00:01:19,550 --> 00:01:26,710
+تنبثق من أهداف طريق التنظيم المجتمع فلابد
+
+12
+00:01:26,710 --> 00:01:32,470
+أن يكون هناك توافق ما بين أهداف الجهاز الجهاز طريق
+
+13
+00:01:32,470 --> 00:01:36,210
+التنظيم المجتمع وما بين أهداف الطريقة أهداف طريق
+
+14
+00:01:36,210 --> 00:01:41,470
+التنظيم المجتمع وأيضًا أهداف طريق التنظيم المجتمع
+
+15
+00:01:41,470 --> 00:01:47,250
+أيضًا هي نفسها أهداف مهنة الخدمة الاجتماعيةالأمور
+
+16
+00:01:47,250 --> 00:01:52,390
+هذه بدنا نعرفها، إذا أجهزة تنظيم المجتمع تنبثق من
+
+17
+00:01:52,390 --> 00:01:59,430
+طريق تنظيم المجتمع واهدافها واهداف طريق تنظيم
+
+18
+00:01:59,430 --> 00:02:03,610
+المجتمع تنبثق من مهنة الخدمة الاجتماعية لأنها نفس
+
+19
+00:02:03,610 --> 00:02:08,250
+الأهدافبينها لأنها طريقة من طرق الخدمة الاجتماعية
+
+20
+00:02:08,250 --> 00:02:14,510
+طريقة تنظيم المجتمع وأيضا الكل سواء الأجهزة أو
+
+21
+00:02:14,510 --> 00:02:19,770
+الطريقة أو المهنة مهنة الخدمة الاجتماعية كل ذلك
+
+22
+00:02:19,770 --> 00:02:25,150
+أهدافهم تتبسك من أين؟ من المجتمعفلذلك لابد أن يكون
+
+23
+00:02:25,150 --> 00:02:30,410
+هناك تطابق ما بين أهداف جهاز تنظيم المجتمع وأهداف
+
+24
+00:02:30,410 --> 00:02:34,750
+الطريقة ومهنة الخدمة الاجتماعية وما بين أهداف
+
+25
+00:02:34,750 --> 00:02:39,570
+المجتمع وأن لا يكون هناك تناقض بين هذه الأهداف
+
+26
+00:02:39,570 --> 00:02:43,310
+أهداف واحدة والكل يسعى إلى تحقيقها
+
+27
+00:02:49,490 --> 00:02:52,670
+طبعا احنا في المؤسسات أو في أجهزة تنظيم المجتمع
+
+28
+00:02:52,670 --> 00:02:57,950
+نسعى إلى تحقيق أهداف المجتمع عندما نضع الأهداف
+
+29
+00:02:57,950 --> 00:03:01,390
+الخاصة بالجهاز لابد هذه الأهداف تكون بنباتقة من
+
+30
+00:03:01,390 --> 00:03:06,170
+المجتمع والأجهزة كلها أجهزة طريقة تنظيم المجتمع
+
+31
+00:03:06,170 --> 00:03:13,850
+تسعى إلى تحقيق أهداف المجتمع طيب
+
+32
+00:03:13,850 --> 00:03:18,760
+ما هو الهدف العام لطريقة تنظيم المجتمع؟مين تقول
+
+33
+00:03:18,760 --> 00:03:21,820
+ياخدناها في السنة الأولى، فيه أهداف، طريقة تنظيم
+
+34
+00:03:21,820 --> 00:03:29,160
+المجتمع، ما هو الهدافبالضبط يعني إحنا حددنا الهدف
+
+35
+00:03:29,160 --> 00:03:34,260
+العام لطريقة تنظيم المجتمع هو عبارة عن حل المشكلات
+
+36
+00:03:34,260 --> 00:03:39,980
+وإشباع الحاجات وممكن نقول أيضا عنه الهدف العام
+
+37
+00:03:39,980 --> 00:03:43,640
+لطريقة تنظيم المجتمع هو رفع مستوى معيشة المواطنين
+
+38
+00:03:43,640 --> 00:03:46,860
+فهذا هو الهدف اللي بتسعى إليه طريقة تنظيم المجتمع
+
+39
+00:03:46,860 --> 00:03:53,120
+و بتسعى إليه أي مؤسسة من مؤسسات طريقة تنظيم
+
+40
+00:03:53,120 --> 00:03:53,700
+المجتمع
+
+41
+00:03:56,430 --> 00:04:00,070
+نعم، ما هي التنمية إلى ماذا تسعى؟ ما هي كلها في
+
+42
+00:04:00,070 --> 00:04:02,910
+إطار التنمية، ما هي طريقة تنظيم المجتمع تسعى
+
+43
+00:04:02,910 --> 00:04:06,410
+للتنمية، طب التنمية في إيش؟ في رفع مستوى معيشة
+
+44
+00:04:06,410 --> 00:04:10,890
+المواطنين، رفع مستوى معيشة المواطنين وحل مشكلاتهم
+
+45
+00:04:10,890 --> 00:04:15,530
+وإشباع حاجاتهم، هذا هو الهدف العام لطريقة تنظيم
+
+46
+00:04:15,530 --> 00:04:21,960
+المجتمعالان نتكلم عن متطلبات الأهداف لطريقة تنظيم
+
+47
+00:04:21,960 --> 00:04:29,240
+المجتمع، طبعا لابد ان تتخذ في الاعتبار مجموعة من
+
+48
+00:04:29,240 --> 00:04:35,200
+المتطلبات لتتمكن الأجهزة بتنظيم المجتمع من تحقيق
+
+49
+00:04:35,200 --> 00:04:40,510
+أهدافهااحنا طبعا أخدنا الأهداف بشكل عام، في الفصل
+
+50
+00:04:40,510 --> 00:04:43,890
+الأول أو في الجزء الأول، لكن الآن بنتكلم عن
+
+51
+00:04:43,890 --> 00:04:47,570
+المتطلبات، تكلمنا عن الهدف العام لطريقة تنظيم
+
+52
+00:04:47,570 --> 00:04:52,670
+المجتمع، الآن بدنا ما هي المتطلبات اللازمة لتحقيق
+
+53
+00:04:52,670 --> 00:04:57,170
+الأهداف، ما هي المتطلبات اللازمة لتحقيق الأهداف
+
+54
+00:04:57,860 --> 00:05:01,700
+نعم، بدي كل طالبة تقوللي متطلب من هذه المطالبات،
+
+55
+00:05:01,700 --> 00:05:07,420
+نعم يا بنتي، أيوة عضوية
+
+56
+00:05:07,420 --> 00:05:12,560
+كافية، الحصول و الإبقاء على عضوية كافية، و العمل
+
+57
+00:05:12,560 --> 00:05:19,140
+على اختيار أعضاء جدد، طبقا للمعايير التي تضمها
+
+58
+00:05:19,140 --> 00:05:24,560
+اللي هي أو المرظمة، واضح؟ ماذا يا بنتي؟
+
+59
+00:05:27,660 --> 00:05:33,900
+التدريب أيضا، التدريب الأعضاء، التدريب مهم جدا، و
+
+60
+00:05:33,900 --> 00:05:35,340
+في جهاز تنظيم المجتمع
+
+61
+00:05:40,700 --> 00:05:44,920
+وهذا الشيء طبيعي اللي هو تنمية التفاعل والاتصال ما
+
+62
+00:05:44,920 --> 00:05:50,040
+بين الأعضاء الموجودين في المنظمة وما بين كل مكونات
+
+63
+00:05:50,040 --> 00:05:59,880
+المنظمة طبعا
+
+64
+00:05:59,880 --> 00:06:03,560
+تقسيم العمل مهم جدا أيضا هذه من المتطلبات من أجل
+
+65
+00:06:03,560 --> 00:06:07,120
+تحقيق الأهداف لابد من تقسيم العمل وتوزيعه على
+
+66
+00:06:07,120 --> 00:06:08,960
+العاملين داخل المنظمة
+
+67
+00:06:13,850 --> 00:06:18,050
+طبعا إسناد المسئوليات والأدوار للاعضاء داخل
+
+68
+00:06:18,050 --> 00:06:21,490
+المنظمة حتى يعرف كل عضو من أعضاء المنظمة ما هو
+
+69
+00:06:21,490 --> 00:06:25,850
+المطلوب منه في عمله داخل المنظمة حتى يقوم بتحقيق
+
+70
+00:06:25,850 --> 00:06:29,630
+هذا العمل وهذا مهم جدا اللي هي إسناد الأدوار
+
+71
+00:06:29,630 --> 00:06:32,630
+لأعضاء المنظمة أيوة يا بنت في الآخر
+
+72
+00:06:37,030 --> 00:06:40,610
+تنظيم العلاقات بين مكونات المنظمة ككل، يعني بين
+
+73
+00:06:40,610 --> 00:06:46,010
+أقصامها المختلفة، لابد من تنظيم هذه العلاقات كلها
+
+74
+00:06:46,010 --> 00:06:50,770
+وإحداث تكامل ما بين أقصامها المختلفة، كله من أجل
+
+75
+00:06:50,770 --> 00:06:56,730
+ماذا؟ من أجل تحقيق أهداف المنظمة، ماذا أيضا؟ أيوة
+
+76
+00:06:56,730 --> 00:07:00,490
+بنتي، هناك؟ تنظيم العلاقات بين مكونات المنظمة،
+
+77
+00:07:00,490 --> 00:07:05,650
+نعم، تنظيم العلاقات وإيجاد تكامل ما بينها، نعم،
+
+78
+00:07:05,650 --> 00:07:11,250
+ماذا أيضا؟العمل على أن يتبنى الأعضاء قيمة إجتماعية
+
+79
+00:07:11,250 --> 00:07:13,970
+مشتركة ومن ضمن هذه القيم الموافقة على أهداف
+
+80
+00:07:13,970 --> 00:07:17,330
+المنظمة والعمل من أجلها طبعا إحنا لما نعمل في أي
+
+81
+00:07:17,330 --> 00:07:21,670
+مؤسسة أو منظمة أو هيئة أو جهاز من أجهزة تنظيم
+
+82
+00:07:21,670 --> 00:07:26,690
+المجتمع لابد أن نعرف ما هي أهداف هذه المنظمة أو
+
+83
+00:07:26,690 --> 00:07:32,050
+المؤسسةويكون كل موظف أو عامل داخل الموظمة يعلم
+
+84
+00:07:32,050 --> 00:07:36,710
+بهذه الأهداف، لماذا؟ حتى يعرف هذه الأهداف ويقوم
+
+85
+00:07:36,710 --> 00:07:40,430
+على تحقيقهاوما هو المطلوب منهم في تحقيق هذه
+
+86
+00:07:40,430 --> 00:07:44,470
+الأهداف فلذلك لابد من الإلمان بأهداف المنظمة عندما
+
+87
+00:07:44,470 --> 00:07:49,690
+نذهب للمؤسسة نتدرب فيها أو نعمل فيها لابد أن نعرف
+
+88
+00:07:49,690 --> 00:07:53,570
+ما هي هذه المؤسسة وما طبيعة عملها وما هي الأهداف
+
+89
+00:07:53,570 --> 00:07:57,150
+الخاصة بهذه المنظمة حتى نقوم نحن من خلال تدريبنا
+
+90
+00:07:57,150 --> 00:08:01,850
+أو عملنا على تحقيق هذه الأهدافوهذا الشيء اللي أول
+
+91
+00:08:01,850 --> 00:08:06,710
+ما الموظف اذهب للمؤسسة لابد يتعرف عليها وعلى
+
+92
+00:08:06,710 --> 00:08:10,110
+أهدافها بالدرجة الأولى وما طبيعة الخدمات التي
+
+93
+00:08:10,110 --> 00:08:14,290
+تقدمها ومن هم المستفيدين من خدماتها، هذا كله لابد
+
+94
+00:08:14,290 --> 00:08:19,010
+أن يلوم بها الموظف وهذا متطلب من متطلبات لازمة
+
+95
+00:08:19,010 --> 00:08:24,610
+لتحقيق أهداف المنظمة أيوة يا بنتي العمل على تصور
+
+96
+00:08:24,610 --> 00:08:27,030
+المنظمة على الموارد التي تحتاجها من البيئة
+
+97
+00:08:27,030 --> 00:08:32,410
+الاجتماعيةوهذا مهم جدا، المنظمة أحيانا تحتاج
+
+98
+00:08:32,410 --> 00:08:37,270
+لموارد، تحتاج لإمكانيات، فلابد أن تكون على علاقة
+
+99
+00:08:37,270 --> 00:08:40,770
+طيبة مع المجتمع الذي توجد فيه، فلذلك من هنا
+
+100
+00:08:40,770 --> 00:08:45,390
+ميزانيتها، مواردها، إمكانياتها المادية والبشرية
+
+101
+00:08:45,390 --> 00:08:49,010
+تعتمد كثيرا على موارد المجتمع وعلى إمكانيات
+
+102
+00:08:49,010 --> 00:08:52,890
+المجتمع، ولابد أن تعلم المنظمة ما هي موارد المجتمع
+
+103
+00:08:53,260 --> 00:08:56,560
+وما هي الإمكانيات وكيف تستفيد من هذه الموارد
+
+104
+00:08:56,560 --> 00:09:00,500
+والإمكانيات اللازمة للمجتمع حتى تكون في العملية
+
+105
+00:09:00,500 --> 00:09:04,720
+الإيه المدخلات لهذه المؤسسة وبعد ذلك تخرج على شكل
+
+106
+00:09:04,720 --> 00:09:09,120
+مخرجات حتى تقول هناك دورة مستمرة ما بين المدخلات
+
+107
+00:09:09,120 --> 00:09:13,900
+من موارد المجتمع وأيضا المخرجات من خدمات المؤسسة
+
+108
+00:09:13,900 --> 00:09:19,160
+وبرامج المؤسسة ومشاريع للمجتمع هذه كل ما يلزم من
+
+109
+00:09:19,160 --> 00:09:23,830
+متطلبات ماذا أيضا؟أيوة بنتى؟ إيجاد الوسائل اللازمة
+
+110
+00:09:23,830 --> 00:09:27,270
+لتنظيم عملية اتخاذ القرارات في المنظمة الوسائل
+
+111
+00:09:27,270 --> 00:09:31,790
+اللازمة لتنظيم عملية اتخاذ القرارات وطبعا مهم جدا
+
+112
+00:09:31,790 --> 00:09:35,610
+اتخاذ القرارات وين هتتم؟ كيف؟ ع طريق لجان؟ ع طريق
+
+113
+00:09:35,610 --> 00:09:40,570
+مجلس إدارة؟ من هم الذين يقومون باتخاذ القرارات
+
+114
+00:09:40,570 --> 00:09:44,930
+داخل المؤسسة؟ هذه مهم جدا اتخاذ القرارات الأشخاص
+
+115
+00:09:44,930 --> 00:09:48,650
+الذين يتخذون القرارات ومسئولياتهم ووظائفهم داخل
+
+116
+00:09:48,650 --> 00:09:54,180
+المؤسسة من هم الذين يتخذون القرارات؟واضح؟ هذه كلها
+
+117
+00:09:54,180 --> 00:09:59,320
+لابد أن يعني تكون معروفة، حتى تستطيع المؤسسة أن
+
+118
+00:09:59,320 --> 00:10:03,160
+تحقق أهدافها، لأن بدون معرفة هذه الأشياء لايمكن أن
+
+119
+00:10:03,160 --> 00:10:07,740
+تحقق المؤسسة أهدافها، أيوة يا بنتي، في الآخر إيش
+
+120
+00:10:07,740 --> 00:10:15,080
+كمان فيه بعد ذلك؟ غمزق بين الأنشطة التنظيمية بحسب
+
+121
+00:10:15,080 --> 00:10:19,840
+سعداد التنفيذ علىالأنشطة التنظيمية نعم التنسيق
+
+122
+00:10:19,840 --> 00:10:24,240
+بينها وطبعا تنسيق بين أعمال المنظمة وأنشطتها
+
+123
+00:10:24,240 --> 00:10:27,860
+المختلفة مهم جدا لابد أن ننسق بين أعمالها و بين
+
+124
+00:10:27,860 --> 00:10:32,800
+أنشطتها المختلفة حتى يمكننا تحقيق الأهداف الخاصة
+
+125
+00:10:32,800 --> 00:10:37,760
+بالمنظمة لأن بدون التنسيقالأمور بتكون متشعبة و
+
+126
+00:10:37,760 --> 00:10:42,140
+تحدث فوضى داخل المؤسسة ولا يمكن إنجاز أي شيء من
+
+127
+00:10:42,140 --> 00:10:46,420
+الأعمال فلذلك التنسيق مهم جدا في البرامج و الخدمات
+
+128
+00:10:46,420 --> 00:10:49,420
+و في الأنشطة اللي بتردما دي المؤسسة كله يعني
+
+129
+00:10:49,420 --> 00:10:53,200
+التنسيق بين الأفراد كذلك و الأعمال المطلوبة من كل
+
+130
+00:10:53,200 --> 00:10:56,920
+فرد داخل المنظمة و الأقسام الخاصة بالمنظمة هذه
+
+131
+00:10:56,920 --> 00:11:00,960
+كلها لابد من التنسيق بينها حتى نستطيع نوفر الوقت
+
+132
+00:11:01,260 --> 00:11:06,000
+والجهد والتكاليف وأن لا تحدث ازدواجية في تادية
+
+133
+00:11:06,000 --> 00:11:12,760
+الأعمال لأن عدم التنسيق قد يؤدي إلى عدم الازدواجية
+
+134
+00:11:12,760 --> 00:11:17,440
+كيف تكون هذه الازدواجية؟ ممكن موظف يقوم بعمل وموظف
+
+135
+00:11:17,440 --> 00:11:21,180
+آخر يقوم بنفس العمل، طب لماذا؟إذا هنا لابد من
+
+136
+00:11:21,180 --> 00:11:25,200
+التنسيق أو قسم يقوم بتحقيق أهداف معينة وقسم آخر
+
+137
+00:11:25,200 --> 00:11:28,560
+يقوم بنفس تحقيق الأهداف طب لأ إذا هنا لابد من
+
+138
+00:11:28,560 --> 00:11:32,500
+التنسيق بين الأقصام المختلفة داخل المؤسسة حتى لا
+
+139
+00:11:32,500 --> 00:11:37,640
+تحدث الإزدواجية في تحقيق الأهداف ونوفر ما قلنا من
+
+140
+00:11:37,640 --> 00:11:42,420
+وقت وجهد وتكاليف ماذا أيضا؟ أيوة بنتي
+
+141
+00:11:45,410 --> 00:11:50,430
+مهمة جدا هذه، هذه مهمة جدا أيضا، لأن كيف أنا أطلب
+
+142
+00:11:50,430 --> 00:11:54,470
+من أفراد المنظمة أو الجهاز التنظيم المجتمع أن يحقق
+
+143
+00:11:54,470 --> 00:11:59,050
+أهداف الجهاز ولا يستطيع الجهاز أن يحقق أهداف
+
+144
+00:11:59,050 --> 00:12:04,870
+العاملينفيه فلذلك من هنا لابد أن نهتم بمشكلات
+
+145
+00:12:04,870 --> 00:12:10,770
+العاملين ومعرفة حاجاتهم وما هي متطلباتهم ويقوم أو
+
+146
+00:12:10,770 --> 00:12:16,170
+تقوم هذه المؤسسة أو هذه المنظمة على إشباع هذه
+
+147
+00:12:16,170 --> 00:12:21,170
+الحاجات وحل المشكلات الخاصة بالعملين داخل المنظمة
+
+148
+00:12:21,170 --> 00:12:26,920
+وإذا لم نهتمفالأمور يعني قد تتفاقم و قد ينتج عنها
+
+149
+00:12:26,920 --> 00:12:32,360
+أشياء سلبية، فلذلك المؤسسة الناجحة هي التي تحسب
+
+150
+00:12:32,360 --> 00:12:37,580
+العاملين لديهاهي التي تحس بمتطلبات العاملين لديها
+
+151
+00:12:37,580 --> 00:12:42,700
+وتقوم على الاستماع لهم للعملين جميعا داخل هذه
+
+152
+00:12:42,700 --> 00:12:47,760
+المنظمة ومعرفة مشكلاتهم واحتياجاتهم وتقوم هذه
+
+153
+00:12:47,760 --> 00:12:51,500
+المنظمة بحل هذه المشكلات وإشباع الاحتياجات الخاصة
+
+154
+00:12:51,500 --> 00:12:56,300
+بالعملين حتى يستطيع العاملون داخل المؤسسة أو
+
+155
+00:12:56,300 --> 00:13:01,120
+المرظمة القيام بواجباتهم على أكمل وجه ممكن وتحقيق
+
+156
+00:13:01,120 --> 00:13:06,220
+أهداف المنظمةفلذلك من هنا هذه المتطلبات المادية
+
+157
+00:13:06,220 --> 00:13:11,340
+والمعنوية مهمة جدا أن نتعرف عليها لدى العاملين
+
+158
+00:13:11,340 --> 00:13:16,900
+والمسؤولين لدى المنظمة أن يقوموا بحل هذه المشكلات
+
+159
+00:13:16,900 --> 00:13:21,410
+إذا كانت توجد مشكلاتوإشباع الحاجات إذا كانت توجد
+
+160
+00:13:21,410 --> 00:13:25,610
+لهم حاجات أيضًا والاستماع والانصات لهم جيدًا
+
+161
+00:13:25,610 --> 00:13:29,650
+والتفاهم معهم جيدًا مع العاملين وأن يكون هناك
+
+162
+00:13:29,650 --> 00:13:34,330
+تقارب ما بين الإدارة والعملين داخل المنظمة حتى
+
+163
+00:13:34,330 --> 00:13:38,230
+تستطيع المنظمة الوصول إلى تحقيق أهدافها بأعلى
+
+164
+00:13:38,230 --> 00:13:43,810
+كفاءة ويعني تكون أيضًا حققت المطلوب من أهدافها
+
+165
+00:13:43,810 --> 00:13:50,860
+بشيء يعني ممتاز داخل المجتمعولذلك الاهتمام بالعمل
+
+166
+00:13:50,860 --> 00:13:55,480
+أهم من الاهتمام بالمبنى المبنى شئ مادي ممكن أبنيه
+
+167
+00:13:55,480 --> 00:13:59,720
+في ست شهور كمدرسة ممكن أبنيها في ست شهور ولكن
+
+168
+00:13:59,720 --> 00:14:05,900
+المعلم .. كم محتاج من الوقت حتى أبني المعلم؟ أربع
+
+169
+00:14:05,900 --> 00:14:11,280
+سنينمين بتقول كام سنة؟ احتاج المعلم، المعلم كام
+
+170
+00:14:11,280 --> 00:14:17,800
+سنة؟ ايوة، حسنتى، المعلم يحتاج منذ ولادة، منذ ان
+
+171
+00:14:17,800 --> 00:14:22,310
+يولد، لابد نعتني به و هو في الراوضةفانت كطفل ثم
+
+172
+00:14:22,310 --> 00:14:25,930
+اعتني بيه في مرحلة الإبتدائية ثم الإعدادية ثم
+
+173
+00:14:25,930 --> 00:14:31,370
+الثانوية ثم الجامعة لكي يقعده معلم لمرحلة في
+
+174
+00:14:31,370 --> 00:14:34,490
+التعليم العام سواء كان في مرحلة الإبتدائية أو
+
+175
+00:14:34,490 --> 00:14:39,530
+الإعدادية أو الثانوية كام سنة؟ 22 سنة هذه للمعلم
+
+176
+00:14:39,530 --> 00:14:46,100
+فقط المهندس يزيده سنة أخرىو الطبيب يزيده سنتان و
+
+177
+00:14:46,100 --> 00:14:50,660
+هكذا حتى اعد الطبيب او المهندس او المعلم احتاج مش
+
+178
+00:14:50,660 --> 00:14:53,780
+اقل من اثنين، ثلاث عشرين سنة، اربع عشرين سنة، خمس
+
+179
+00:14:53,780 --> 00:14:59,820
+عشرين سنة و انا بعد هذا المعلم في حين المدى المبنى
+
+180
+00:14:59,820 --> 00:15:03,820
+اعده في ستة شهور مدرسة ممكن تنبني يعني في اقصى وقت
+
+181
+00:15:03,820 --> 00:15:11,320
+سنة مع قلة الممكنية سنة ممكن لكن المعلم يحتاج لوقت
+
+182
+00:15:11,320 --> 00:15:17,120
+طويلفلذلك من هنا الاهتمام بالجوانب المعنوية أحيانا
+
+183
+00:15:17,120 --> 00:15:20,820
+قد يكون أو التدريب والتعليم أكثر من الجوانب
+
+184
+00:15:20,820 --> 00:15:24,240
+المادية الجوانب المادية من السهل أن نحصل عليها
+
+185
+00:15:24,240 --> 00:15:29,580
+ولكن الجوانب الإنسانية والمهنية من الصعب أن نحصل
+
+186
+00:15:29,580 --> 00:15:34,680
+عليهاواضح هذه الأمور لابد أن نفهمها وكذلك مثل آخر
+
+187
+00:15:34,680 --> 00:15:38,960
+إعداد المصانع سهل ولكن إعداد العاملين في المصانع
+
+188
+00:15:38,960 --> 00:15:42,980
+هو الصعب إعداد المهندسين و العمال و القبراء و
+
+189
+00:15:42,980 --> 00:15:49,560
+الفنيين داخل المصانع المختلفة هو الصعب أما المصنع
+
+190
+00:15:49,560 --> 00:15:53,380
+من السهل أن نبني المصنع و نأتي بالآلات من السهل
+
+191
+00:15:53,380 --> 00:15:59,950
+جدالكن أن نعد الإنسان المهني، أن نعد المهندس، أن
+
+192
+00:15:59,950 --> 00:16:05,370
+نعد الفني، هذا هو الصعب الذي يحتاج إلى وقت و بناء،
+
+193
+00:16:05,370 --> 00:16:09,490
+فلذلك من هنا لابد من التركيز على العنصر الإنساني،
+
+194
+00:16:09,490 --> 00:16:13,150
+لابد من التركيز على العنصر الإنساني و نهتم بهذا
+
+195
+00:16:13,150 --> 00:16:20,910
+الإنسانحتى نستطيع أن هذا الإنسان يستطيع أن يؤثر في
+
+196
+00:16:20,910 --> 00:16:24,310
+مجتمعه من خلال مؤسسته من خلال ما يقوم بيه من
+
+197
+00:16:24,310 --> 00:16:30,070
+الأعمال بجد وإجتهاد هذا هو الجانب اللي هو إشباع
+
+198
+00:16:30,070 --> 00:16:33,950
+الحاجات المادية والمعنوية الخاصة بالعملين داخل
+
+199
+00:16:33,950 --> 00:16:39,530
+المنظمة وأن نهتم بهم نهتم بهم ماديا ومعنويا
+
+200
+00:16:39,530 --> 00:16:45,590
+الإنسان أسمى شيء في الوجودفلذلك لابد أن نهتم بهذا
+
+201
+00:16:45,590 --> 00:16:53,990
+العنصر الإنساني في
+
+202
+00:16:53,990 --> 00:16:57,850
+عندنا أيضا للمنظمة .. أيوة تفضلي
+
+203
+00:17:01,460 --> 00:17:06,000
+نعم الأخطار الخارجية وهدولة أيضا يعني نفس الشيء
+
+204
+00:17:06,000 --> 00:17:11,380
+علشان تستطيع المنظمة تحقيق هدافة لابد أن يعني نحمل
+
+205
+00:17:11,380 --> 00:17:15,480
+منظمة من الأخطار الخارجية التي قد تهدد هذه المنظمة
+
+206
+00:17:15,480 --> 00:17:20,320
+أحيانا في أخطار خارجية و أخطار داخلية لابد أن نهتم
+
+207
+00:17:20,320 --> 00:17:24,780
+بهذه الأخطار من الناحيتينمش هي الأخطار الخارجية؟
+
+208
+00:17:24,780 --> 00:17:29,440
+الأخطار الخارجية نعم في أخطار خارجية أحيانا يعني
+
+209
+00:17:29,440 --> 00:17:33,320
+ليها المنظمة قد تهددها من بعض المنظمات المنظمة
+
+210
+00:17:33,320 --> 00:17:37,300
+تهدد منظمة أخرى هذا خطر خارجي منافسة لها تؤدي
+
+211
+00:17:37,300 --> 00:17:41,800
+للصراع تؤدي للقضاء المنظمة ممكن وضع سياسي عام ممكن
+
+212
+00:17:41,800 --> 00:17:45,420
+واحد ممكن ممكن برضه هذه كلها تؤثر على المنظمة
+
+213
+00:17:45,420 --> 00:17:49,640
+فالأخطار الخارجية يعني لابد أن يكون الإدارة تكون
+
+214
+00:17:49,640 --> 00:17:52,910
+متفاهمة لهذه الأخطار الخارجيةهل ما بيصير في مصر
+
+215
+00:17:52,910 --> 00:17:56,430
+بيساكروا الجمعيات و المؤسسات اللي .. ممكن الدولة
+
+216
+00:17:56,430 --> 00:18:01,870
+نفسها أحيانا قد يعني تجد المنظمة لاتناسبها فممكن
+
+217
+00:18:01,870 --> 00:18:06,330
+أن تلغيها في المجتمع فلذلك من هنا لابد المنظمة أن
+
+218
+00:18:06,330 --> 00:18:11,590
+تتفهم هذه الأخطار الخارجية وهل المنظمة تقوم بتحقيق
+
+219
+00:18:11,590 --> 00:18:13,130
+أهداف المجتمع أم لا؟
+
+220
+00:18:15,980 --> 00:18:19,800
+إذا المنظمة لم تقم بهداف المجتمع، هذا نوع خطر
+
+221
+00:18:19,800 --> 00:18:24,180
+يُعتبر، داخلي أيضًا، ليه؟ لأن المنظمة تعمل أشياء
+
+222
+00:18:24,180 --> 00:18:28,100
+لا يرضى عنها المجتمع، إذا هنا لا تستمر، لابد أن
+
+223
+00:18:28,100 --> 00:18:31,620
+يكون هناك توافق ما بين أهدافها واهداف المجتمع اللي
+
+224
+00:18:31,620 --> 00:18:36,820
+توجد فيه، لابد أن يكون هناك توافق، فلذلك المنظمة
+
+225
+00:18:36,820 --> 00:18:42,560
+الناجحة هي التي تنظر وترى وتتفحص وتعرف ما هي
+
+226
+00:18:42,560 --> 00:18:48,190
+المشكلات الخارجيةوتحاول أن تتغلب عليها ما هو الخطر
+
+227
+00:18:48,190 --> 00:18:54,470
+الخارجي وتحاول أن تتغلب عليه واضح؟ وكذلك في داخل
+
+228
+00:18:54,470 --> 00:18:58,830
+المنظمة تعرف من هم في صف المنظمة ومنهم خارج
+
+229
+00:18:58,830 --> 00:19:03,470
+المنظمة هذه اللي هي المؤسسة ولابد من أجل تحقيق
+
+230
+00:19:03,470 --> 00:19:08,570
+الأهداف أن نعرف ما هو مع ومن هو ضد حتى نستطيع أن
+
+231
+00:19:08,570 --> 00:19:13,310
+نسير بأهداف المنظمة على خير ما يرام طبق الأهداف
+
+232
+00:19:13,310 --> 00:19:19,840
+المجتمعةهذه أمور أيضًا من المتطلبات اللي هي تحقيق
+
+233
+00:19:19,840 --> 00:19:23,200
+أهداف المنظمة حمايتها من الأخطار الخارجية
+
+234
+00:19:23,200 --> 00:19:30,440
+والداخلية واضح؟ واحيانًا مؤسساتنا ومنظماتنا تكون
+
+235
+00:19:30,440 --> 00:19:35,580
+معرضة من أخطار خارجية من أعداء من أعداء أيضًا لنا
+
+236
+00:19:35,580 --> 00:19:41,120
+لذلك لابد أن نتفهم هذه الأخطار ونقل المؤسسة أو
+
+237
+00:19:41,120 --> 00:19:42,620
+المنظمة من هذه الأخطار
+
+238
+00:19:48,340 --> 00:19:53,540
+ماذا أيضًا متطلبات لازمة للمنظمة؟ ما اتكلمتش
+
+239
+00:19:53,540 --> 00:19:57,620
+بالمرة، أيه يا بنتي؟ إيجاد قوانين لوائح ونظمة
+
+240
+00:19:57,620 --> 00:20:03,800
+قوانين المؤسسة طبعًا
+
+241
+00:20:03,800 --> 00:20:10,040
+اللي هو الضبط يعني القوانين إيجاد قوانين لوائح
+
+242
+00:20:10,040 --> 00:20:18,360
+ونظمة قوانين المؤسسة ضبط حتى نضمن حتى نضمنسير
+
+243
+00:20:18,360 --> 00:20:22,960
+المنظمة على خير ما يرام وأن هناك ضوابط وقوانين
+
+244
+00:20:22,960 --> 00:20:28,840
+ونظم ولوائع تنظم عمل المنظمة فلذلك من هنا لابد من
+
+245
+00:20:28,840 --> 00:20:33,420
+إيجاد هذه النظم والقوانين واللوائع الخاصة بالمنظمة
+
+246
+00:20:36,510 --> 00:20:39,350
+وهذا ما نسميه ضبط الاجتماعي أحيانًا الضبط
+
+247
+00:20:39,350 --> 00:20:43,990
+الاجتماعي ليس قوانين ونظم لوائح رسمية أحيانًا قد
+
+248
+00:20:43,990 --> 00:20:48,150
+يكون عادات وتقاليد وأعراف اعتادوا عليها العاملين
+
+249
+00:20:48,150 --> 00:20:53,490
+داخل المؤسسة لابد أن تكون هذه العادات والتقاليد
+
+250
+00:20:53,490 --> 00:20:58,770
+منظمة أيضًا و لا تتعارض مع قوانين المنظمة يسير
+
+251
+00:20:58,770 --> 00:21:03,220
+عليها العاملون داخل المنظمةفالضبط يشمل للناحيتين
+
+252
+00:21:03,220 --> 00:21:07,180
+الرسمي والغير رسمي و أحيانا قد تنشأ أيضا جامعات
+
+253
+00:21:07,180 --> 00:21:10,860
+غير رسمية أو علاقات غير رسمية داخل المنظمة هذه
+
+254
+00:21:10,860 --> 00:21:15,600
+لابد أن نستغلها و نستثمرها لصالح المنظمة وهذه كانت
+
+255
+00:21:15,600 --> 00:21:18,460
+في التنظيم الغير رسمي لأن أحيانا يوجد تنظيم رسمي
+
+256
+00:21:18,460 --> 00:21:22,880
+في المؤسسة و تنظيم غير رسمي ينشأ .. مين تقولي ما
+
+257
+00:21:22,880 --> 00:21:26,820
+هو التنظيم الرسمي و التنظيم الغير رسمي؟ مادرستوش
+
+258
+00:21:26,820 --> 00:21:33,060
+.. ايوة تفضل يا بنتيالمؤسسة ولوائح المنظمة اللي هو
+
+259
+00:21:33,060 --> 00:21:39,300
+نفس العدد العالي؟ لأ هو التنظيم الرسمي هو الذي
+
+260
+00:21:39,300 --> 00:21:43,800
+ينشأ داخل المنظمة طبقا للقوانين يعني الموظفين يتم
+
+261
+00:21:43,800 --> 00:21:49,060
+اختيارهم حسب ماذا داخل المؤسسة حسب القوانين والنظم
+
+262
+00:21:49,060 --> 00:21:53,100
+واللوائح اللي بتنظم الحاجة إلى الموظف داخل المنظمة
+
+263
+00:21:53,560 --> 00:21:58,100
+لكن أحياناً في تنظيمات غير رسمية تنشأ داخل التنظيم
+
+264
+00:21:58,100 --> 00:22:01,220
+الرسمي، يعني بين العاملين اللي تم تعيينهم في
+
+265
+00:22:01,220 --> 00:22:04,720
+المؤسسة، نتيجة ماذا؟ نتيجة العلاقات الاجتماعية
+
+266
+00:22:04,720 --> 00:22:08,940
+التي تحدث بين العاملين، تنشأ هناك جماعات، بنسميها
+
+267
+00:22:08,940 --> 00:22:15,540
+جماعات غير رسمية، تنشأ يعني داخل المؤسسة، هذه لابد
+
+268
+00:22:15,540 --> 00:22:19,320
+على الإدارة أن تستثمر هذه الجماعات الغير رسمية
+
+269
+00:22:19,320 --> 00:22:25,600
+لصالحالعمل لصالح عمل المنظمة وتحقيق أهداف المنظمة
+
+270
+00:22:25,600 --> 00:22:30,340
+وإلا تكون هذه الجامعات الغير رسمية لها انحرافات
+
+271
+00:22:30,340 --> 00:22:34,480
+سلوكية انحرافات قد تؤدي إلى تعطيل الأعمال لأ في
+
+272
+00:22:34,480 --> 00:22:37,580
+حالة لو كانت هذه الجامعات تؤدي إلى تعطيل الأعمال
+
+273
+00:22:37,580 --> 00:22:41,060
+لابد من وقفهاولكن إذا كانت هذه الجماعات اللي بتنشأ
+
+274
+00:22:41,060 --> 00:22:45,380
+نتيجة العلاقات الاجتماعية تساعد وتساهم في إنجاز
+
+275
+00:22:45,380 --> 00:22:50,020
+العمل وتحقيق أهداف المنظمة على خير ما يُرام علينا
+
+276
+00:22:50,020 --> 00:22:53,560
+أن نشجع هذه الجماعات الغير رسمية داخل التنظيم
+
+277
+00:22:53,560 --> 00:22:57,700
+الرسمي أو داخل الجماعة الرسمية في المؤسسة حتى
+
+278
+00:22:57,700 --> 00:23:03,980
+نستطيع أن نحقق أهداف المؤسسة على خير ما يُرامطيب
+
+279
+00:23:03,980 --> 00:23:07,820
+في عندنا أيضا ما زلنا في المتطلبات أيوة يا بنتى
+
+280
+00:23:07,820 --> 00:23:13,920
+نعم
+
+281
+00:23:13,920 --> 00:23:17,780
+طبعا
+
+282
+00:23:17,780 --> 00:23:21,680
+هذه الوسائل اللى بتعالج الصراعات وهذه مهمة جدا نعم
+
+283
+00:23:21,680 --> 00:23:26,880
+احنا مابندناش اشرح هذه الوسائل طيب كيف بندناش نجيب
+
+284
+00:23:26,880 --> 00:23:29,720
+الوسائل اللى بتقدر على الصراعات يا بنتى يلا المهم
+
+285
+00:23:29,720 --> 00:23:32,620
+احنا نفه من البنت كيف بندناش نقدر على الصراعات
+
+286
+00:23:39,680 --> 00:23:42,280
+نعم وسائل هذه برضه من ضمن الوسائل
+
+287
+00:23:45,190 --> 00:23:48,710
+نعم، نعم، ماذا يا بنتي؟ ممكن لو كان في أكتر من
+
+288
+00:23:48,710 --> 00:23:53,950
+عامل أو موظف بتنافسوا على منصب معين من خلال
+
+289
+00:23:53,950 --> 00:23:57,590
+انتخابات مثلا في المؤسسة ممكن، ممكن، وسيلة من
+
+290
+00:23:57,590 --> 00:24:02,030
+الوسائل، ممكن، هاه، هذه كلها يعني أننا نستطيع أن
+
+291
+00:24:02,030 --> 00:24:05,350
+نبتكر، ماذا أيضا؟ مين تقولي أيضا ماذا من وسائل
+
+292
+00:24:05,350 --> 00:24:08,370
+ممكن نستخدمها في القضاء على الصراعات بين العاملين
+
+293
+00:24:08,370 --> 00:24:11,800
+داخل المؤسسة من أجل تحقيق أهداف المؤسسة؟قامت
+
+294
+00:24:11,800 --> 00:24:17,340
+لقاءات ومناقشات وحوافظ، اتحفظت .. لقاءات ومناقشات،
+
+295
+00:24:17,340 --> 00:24:21,700
+bravo، أحسنت من الوسائل، ماذا أيضًا؟ تحفظ والأشياء
+
+296
+00:24:21,700 --> 00:24:25,100
+بالعمل الجماعي بالعمل الجماعي، تشجيع من العمل
+
+297
+00:24:25,100 --> 00:24:28,560
+الفردي للعمل الجماعي، برضه هادم نقضي على اتصارعات،
+
+298
+00:24:28,560 --> 00:24:35,040
+نعمبرافو الأنشطة مهمة جدا الأنشطة الاجتماعية حفلات
+
+299
+00:24:35,040 --> 00:24:40,900
+و رحلات و زيارات هذه كلها تساعد على الحد من إيه من
+
+300
+00:24:40,900 --> 00:24:46,020
+الصراعات داخل المؤسسة وتعمل على إيجاد التقالب
+
+301
+00:24:46,020 --> 00:24:51,040
+والترابط والمحبة بين العملين داخل المنظمة هذه كلها
+
+302
+00:24:51,040 --> 00:24:54,240
+نستطيع النقض على الصراعات فلذلك من أجل تحقيق هدا
+
+303
+00:24:54,240 --> 00:24:57,600
+في المؤسسة أو المنظمة لابد أن نقض على الصراعات
+
+304
+00:24:57,600 --> 00:25:01,860
+الداخليةداخل .. بين العاملين، داخل المنظمة، حتى
+
+305
+00:25:01,860 --> 00:25:06,420
+الجميع يتعاون من أجل تحقيق أهداف المنظمة ماذا
+
+306
+00:25:06,420 --> 00:25:15,140
+أيضا؟ مين ما اتكلمتش نهائيا؟ أيه و أيه منكم؟ وحدة
+
+307
+00:25:15,140 --> 00:25:20,180
+المنظمات وتكاملها، العمل على تنمية وحدة المنظمات
+
+308
+00:25:20,180 --> 00:25:24,780
+طبعا اللي هي العمل دائما على إن المنظمة بنخليها في
+
+309
+00:25:24,780 --> 00:25:29,680
+وحدة متكاملةما تكون هناك الأقسام مفرقة أو الوحدات
+
+310
+00:25:29,680 --> 00:25:35,260
+مفرقة، كلها تكمل بعضها أيضا، أن يكون هناك كل قسم
+
+311
+00:25:35,260 --> 00:25:40,820
+أو كل وحدة داخل المنظمة تقوم بعمل يكمل العمل الآخر
+
+312
+00:25:40,820 --> 00:25:46,620
+مع الوحدة الأخرىوهذا من المتطلبات اللازمة لتحقيق
+
+313
+00:25:46,620 --> 00:25:50,760
+أهداف المؤسسة حتى يكون هناك تكامل بين أقسامها
+
+314
+00:25:50,760 --> 00:25:55,720
+ووحداتها المختلفة وإلا يكون كل قسم أو وحدة منفصلة
+
+315
+00:25:55,720 --> 00:26:01,200
+عن الأخرى لابد الكل يتفهم ما هو المطلوب منه داخل
+
+316
+00:26:01,200 --> 00:26:04,860
+هذه المؤسسة من الوحدات والأقسام المختلفة حتى
+
+317
+00:26:04,860 --> 00:26:08,800
+الجميع يعمل على تحقيق الأهداف العامة للمؤسسة أو
+
+318
+00:26:08,800 --> 00:26:11,700
+المنظمة ماذا أيضا؟ أيوة يا بنتي
+
+319
+00:26:18,240 --> 00:26:24,840
+هذا مهم جدا ال event أيضا طبعا طبعا طبعا هذه مهمة
+
+320
+00:26:24,840 --> 00:26:30,160
+جدا الوسائل اللي ممكن نغير من أهداف المنظمةمن بناء
+
+321
+00:26:30,160 --> 00:26:35,720
+المنظمة، نغير أيضًا في الموارد كذلك، نعمل على
+
+322
+00:26:35,720 --> 00:26:40,140
+زيادة موارد المنظمة، كيف بدأ نعمل تنميتها،
+
+323
+00:26:40,140 --> 00:26:44,120
+استثمارها في أشياء مفيدة، ربما هذه المنظمة أحيانًا
+
+324
+00:26:44,120 --> 00:26:49,560
+تعتمد على المساعدات والإعاناتفمن أجل التحقيق يمكن
+
+325
+00:26:49,560 --> 00:26:53,320
+تقف هذه المساعدات والإعانات من الجهة المانحة أو من
+
+326
+00:26:53,320 --> 00:26:58,900
+الجهة التي تقدم هذه المساعدة أو المعونة طب هل تقف
+
+327
+00:26:58,900 --> 00:27:03,680
+المؤسسة أو المنظمة يعني من أجل إيقاف هذه المساعدات
+
+328
+00:27:03,680 --> 00:27:08,440
+والمعونات؟ لا طبعا هنا لابد من التفكير في كيفية
+
+329
+00:27:08,440 --> 00:27:14,580
+تنمية مواردها، في كيفية إيجاد مشاريعتنمي مواردها
+
+330
+00:27:14,580 --> 00:27:19,740
+حتى تستطيع أن تلفق من هذه الموارد على من تقوم
+
+331
+00:27:19,740 --> 00:27:23,680
+بخدمتها، يعني مثلا لو كانت جمعية تعمل في رعاية
+
+332
+00:27:23,680 --> 00:27:28,900
+المعاقبوهذه الجمعية تحتاج إلى مساعدات وإعانات
+
+333
+00:27:28,900 --> 00:27:32,880
+وقامت هذه الجمعية واخذت هذه المساعدات والإعانات
+
+334
+00:27:32,880 --> 00:27:38,040
+لكن الجهة التي تمنح أو تعطي هذه المساعدات يعني
+
+335
+00:27:38,040 --> 00:27:41,920
+سواء كانت حكومة أو كانت جهة من الخارج أو من الداخل
+
+336
+00:27:41,920 --> 00:27:46,060
+تمنح هذه المؤسسة الخاصة بالمعاقين من هل تقف هذه
+
+337
+00:27:46,060 --> 00:27:50,780
+المؤسسة عن القيام بعملها الإنسان في رعاية المعاقين
+
+338
+00:27:50,780 --> 00:27:55,670
+وتقديم الخدمات اللازمة لهم؟طبعاً لابد من إيجاد و
+
+339
+00:27:55,670 --> 00:28:00,370
+تفكير في مشاريع استثمارية، مشاريع استثمارية تعود
+
+340
+00:28:00,370 --> 00:28:04,550
+بالفائدة على هذه المؤسسة حتى تنفق على المعاقين
+
+341
+00:28:05,490 --> 00:28:09,190
+الرعاية الاجتماعية حتى تقوم برعايتهم فممكن تعمل أي
+
+342
+00:28:09,190 --> 00:28:13,870
+مورد اقتصادي لتنمية هذه الموارد ممكن تعمل مشروع
+
+343
+00:28:13,870 --> 00:28:18,530
+للأثات زي إرادة مثلا عندها زي مشروع إرادة يعني
+
+344
+00:28:18,530 --> 00:28:24,290
+مشاريع في بناء عمل أثات عمل معجنات عمل يعني إذا
+
+345
+00:28:24,290 --> 00:28:28,130
+كان لديها قطعة أرض أن تستثمرها في الزراعة و هكذا
+
+346
+00:28:28,300 --> 00:28:31,460
+إذا هنا لابد من التفكير في المشاريع الزراعية
+
+347
+00:28:31,460 --> 00:28:35,860
+الصناعية التجارية لماذا حتى الدخل الذي يعود
+
+348
+00:28:35,860 --> 00:28:39,440
+والفائدة التي تعود على المؤسسة تنفقها عالمًا على
+
+349
+00:28:39,440 --> 00:28:44,360
+هذه الفئة المحتاجة للرعاية وهي رعاية المعاقين داخل
+
+350
+00:28:44,360 --> 00:28:48,960
+مجتمعنا وهكذا كل مؤسسة المؤسسة الناجحة هي التي
+
+351
+00:28:48,960 --> 00:28:53,820
+تعتمد على ذاتها وعلى مواردها الذاتية ولا تعتمد على
+
+352
+00:28:53,820 --> 00:28:58,880
+موارد داخلية وخارجية هذه المؤسسة الناجحةالتي
+
+353
+00:28:58,880 --> 00:29:03,900
+تستطيع أن تواجه الصعاب وتستطيع أن تستمر في عطائها
+
+354
+00:29:03,900 --> 00:29:09,440
+المستمر ولذلك من هنا بنقول لابد من اللي هي إيجاد
+
+355
+00:29:09,440 --> 00:29:14,160
+الوسائل اللازمة لتنمية وارد مؤسساتنا حتى تستطيع أن
+
+356
+00:29:14,160 --> 00:29:19,300
+تحقق أهدافها إلى هنا يعني في أي سؤال في الأهداف
+
+357
+00:29:19,300 --> 00:29:22,580
+والمتطلبات الهدف العام لطريقة تنظيم المجتمع
+
+358
+00:29:22,580 --> 00:29:26,640
+والمتطلبات اللازمة لتحقيق أهداف طريقة تنظيم
+
+359
+00:29:26,640 --> 00:29:31,160
+المجتمع بشكل عامطيب إلى هنا ننهي هذه المحاضرة على
+
+360
+00:29:31,160 --> 00:29:34,620
+أمل اللقاء بكنا في المحاضرة القادمة بإذن الله
+
+361
+00:29:34,620 --> 00:29:40,140
+تعالى حول بناء أجهزة تنظيم المجتمع إلى أن نلتقي في
+
+362
+00:29:40,140 --> 00:29:44,560
+المحاضرة القادمة لكنا من كل تحيط وتقدير والسلام
+
+363
+00:29:44,560 --> 00:29:46,840
+عليكم ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwZ8bh257eMcqZ22bPBOhO8z/pL3EJ0M02gY_postprocess.srt b/PL9fwy3NUQKwZ8bh257eMcqZ22bPBOhO8z/pL3EJ0M02gY_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..cff3b80d2031d8f10664c19a0f8934bd99bf3442
--- /dev/null
+++ b/PL9fwy3NUQKwZ8bh257eMcqZ22bPBOhO8z/pL3EJ0M02gY_postprocess.srt
@@ -0,0 +1,1572 @@
+1
+00:00:20,910 --> 00:00:24,270
+بسم الله الرحمن الرحيم الحمد لله رب العالمين
+
+2
+00:00:24,270 --> 00:00:28,810
+والصلاة والسلام على أشرف المرسلين سيدنا محمد وعلى
+
+3
+00:00:28,810 --> 00:00:34,110
+آله وصحبه أجمعين بنات الطالبات السلام عليكم ورحمة
+
+4
+00:00:34,110 --> 00:00:35,490
+الله وبركاته
+
+5
+00:00:37,600 --> 00:00:41,460
+في المحاضرة السابقة كنا نتحدث عن مقهوم التطوع
+
+6
+00:00:41,460 --> 00:00:46,820
+وتحدثنا عن تعريفات التطوع ووصلنا إلى التعريف
+
+7
+00:00:46,820 --> 00:00:52,620
+الإجرائي للتطوع في هذه المحاضرة سنتناول تطور
+
+8
+00:00:52,620 --> 00:00:58,770
+التطوعكيف تطور هذا التطوع والتطوع في الإسلام وفي
+
+9
+00:00:58,770 --> 00:01:04,230
+الأديان الأخرى وكذلك مفهوم الخدمة العامة والتطوع
+
+10
+00:01:04,230 --> 00:01:09,230
+ثم بعد ذلك ننتقل إلى مدرسة الخدمة الاجتماعية
+
+11
+00:01:09,230 --> 00:01:14,150
+الخدمة الاجتماعية والتطوع في هذا الموضوع التطوع
+
+12
+00:01:14,150 --> 00:01:18,450
+والخدمة العامة ما الفرق بين الخدمة العامة والتطوع؟
+
+13
+00:01:20,590 --> 00:01:26,010
+التطوع تحدثنا عنه في المحاضرة السابقة بأنه أي جهد
+
+14
+00:01:26,010 --> 00:01:33,950
+يبذله الإنسان بلا مقابل وأيضا الخدمة العامة أحيانا
+
+15
+00:01:33,950 --> 00:01:39,850
+يعني تبذل بلا مقابل وتبذل بمقابلفلذلك من هنا
+
+16
+00:01:39,850 --> 00:01:45,830
+سنتعرف على الخدمة العامة التطوعية هذه طبعا الخدمة
+
+17
+00:01:45,830 --> 00:01:50,330
+العامة التطوعية هي بلا مقابلة ولكن تكون من قبل
+
+18
+00:01:50,330 --> 00:01:56,690
+المجتمع يعني تؤدى بدافع من الفرد والجماعة والمجتمع
+
+19
+00:01:56,690 --> 00:02:03,550
+للوصول إلى حياة كريمة إلى حياة أفضل مما هو عليها
+
+20
+00:02:03,550 --> 00:02:10,390
+الفرد أو الجماعة أو المجتمعطبعا مثل ذلك الخدمة
+
+21
+00:02:10,390 --> 00:02:15,510
+العامة مثل الجمعيات مثل المؤسسات الاجتماعية هذه
+
+22
+00:02:15,510 --> 00:02:19,990
+المؤسسات الاجتماعية نشأت في المجتمع لإشباح حاجة من
+
+23
+00:02:19,990 --> 00:02:24,590
+حاجات المجتمع و لحل مشكلة من مشكلات المجتمع فلذلك
+
+24
+00:02:24,590 --> 00:02:30,380
+معظم المؤسسات الاجتماعية عملوهايعني تطوعي للقيام
+
+25
+00:02:30,380 --> 00:02:35,840
+بأعمال تطوعية للمجتمع لخدمة المجتمع وحل مشكلات
+
+26
+00:02:35,840 --> 00:02:40,160
+المجتمع وإشباح حاجات المجتمع فلذلك نجد كل معظم
+
+27
+00:02:40,160 --> 00:02:44,200
+المؤسسات الاجتماعية سواء في مجال الشباب في مجال
+
+28
+00:02:44,200 --> 00:02:48,320
+المرأة في مجال المسنين في مجال المعاقين بشكل عام
+
+29
+00:02:48,320 --> 00:02:54,660
+في مجال تنمية المجتمع هي مؤسسات خدماتية خدماتية
+
+30
+00:02:54,660 --> 00:03:00,910
+تقدم خدمة للمجتمعتقدم خدمة للفرج والجماعة والمجتمع
+
+31
+00:03:00,910 --> 00:03:06,790
+بشكل عام فلذلك من هنا الخدمة العامة هي مثل
+
+32
+00:03:06,790 --> 00:03:12,150
+الجمعيات والمؤسسات الاجتماعية التي تقدم خدمات عامة
+
+33
+00:03:12,150 --> 00:03:18,450
+للمجتمع وهذه تقدم بلا مقابل ولذلك بنسميها مؤسسات
+
+34
+00:03:18,450 --> 00:03:23,690
+غير ربحيةمؤسسات غير ربحية هذه الفرق بين المؤسسات
+
+35
+00:03:23,690 --> 00:03:26,810
+الاقتصادية والمؤسسات الاجتماعية بين المؤسسات
+
+36
+00:03:26,810 --> 00:03:30,890
+الاجتماعية غير ربحية ولكن المؤسسات الاقتصادية هي
+
+37
+00:03:30,890 --> 00:03:36,310
+مؤسسات ربحية قائمة على الربح وتحقيق أكبر قدر ممكن
+
+38
+00:03:36,310 --> 00:03:40,490
+من الربح ولذا .. ولذا .. أما مؤسساتنا الاجتماعية
+
+39
+00:03:40,490 --> 00:03:45,230
+فهي مؤسسات خدماتية تقدم خدمات في التعليم في الصحة
+
+40
+00:03:45,230 --> 00:03:51,550
+في غير ذلك واضحفهذه كلها يعني مؤسسات الخدمة العامة
+
+41
+00:03:51,550 --> 00:03:55,250
+التطوعية في عندنا اصطلاح الخدمة العامة بالمفهوم
+
+42
+00:03:55,250 --> 00:03:59,050
+الواسع اللي هي الخدمات اللي تقدم للفرد أو الجماعة
+
+43
+00:03:59,050 --> 00:04:04,230
+أو المجتمع دون مشاركة فعلية من العنصر المقدم إليه
+
+44
+00:04:04,230 --> 00:04:11,430
+هذه الخدمة واضح؟ تعتبر تلك الخدمات من المسئوليات
+
+45
+00:04:11,430 --> 00:04:17,660
+الأساسية للدولة زي خدمة التعليمزي الخدمات الصحية،
+
+46
+00:04:17,660 --> 00:04:21,020
+خدمات الشؤون الاجتماعية، هذه كلها خدمات تقدمها
+
+47
+00:04:21,020 --> 00:04:25,600
+الدولة بلا مقابل خدمات تقبلها التي تقدمها الدولة
+
+48
+00:04:25,600 --> 00:04:31,400
+للفرج والجماعة والمجتمع واضح؟ هذه الخدمة العامة من
+
+49
+00:04:31,400 --> 00:04:39,300
+المفهوم الواثر خدمات تقدمها الدولة في
+
+50
+00:04:39,300 --> 00:04:42,900
+عندنا اصطلاح الخدمة العامة يطلق على تكليف الشباب
+
+51
+00:04:42,900 --> 00:04:47,500
+أحياناًيعني بيطلبوا منهم أحياناً لخراجين أن
+
+52
+00:04:47,500 --> 00:04:52,000
+يشتغلوا لكن ممكن يعطوهم مقابل أحياناً مقابل التطوع
+
+53
+00:04:52,000 --> 00:04:56,280
+الخاص بهذا العمل أو الخدمة التي يقدمونها في بعض
+
+54
+00:04:56,280 --> 00:05:00,260
+الدول لكن إحنا يعني عندنا في مجتمعنا الفلسطيني
+
+55
+00:05:00,260 --> 00:05:04,900
+أحياناً ممكن الجامعات تفرض عدد معين من الساعات
+
+56
+00:05:04,900 --> 00:05:10,200
+التطوعية كخدمة عامة يقوم بها الشباب في الجامعة
+
+57
+00:05:10,200 --> 00:05:15,110
+لتقديم خدمة للوطنللمجتمع اللي يعيشون فيه وهذه
+
+58
+00:05:15,110 --> 00:05:19,730
+بتكون عن طريق الخدمة العامة ساعات تطوعية بلا مقابل
+
+59
+00:05:19,730 --> 00:05:24,850
+ولذلك ربما تحدث عندنا في الجامعة هذه الساعات منذ
+
+60
+00:05:24,850 --> 00:05:28,490
+العام القادم والآن بدأت بعض الأقسام بتطبيقها
+
+61
+00:05:28,490 --> 00:05:32,050
+كتدريب يعني ليس مثل التدريب الميداني اللي احنا
+
+62
+00:05:32,050 --> 00:05:35,550
+بناخده كساعات أكاديمية تدريبية هناك أيضا حوالي
+
+63
+00:05:35,550 --> 00:05:41,370
+أربعين ساعة مطلوب من كل طالب وطالبة أن يؤدون هذه
+
+64
+00:05:41,370 --> 00:05:49,920
+الساعاتالتطوعية خدمة مجانية ويشترط عند التخرج و
+
+65
+00:05:49,920 --> 00:05:53,580
+الحصول على شهادة ال .. الشهادة الأكاديمية ال
+
+66
+00:05:53,580 --> 00:05:58,100
+baccalaureate أن يكون الطالب متطوع للعمل في إحدى
+
+67
+00:05:58,100 --> 00:06:02,380
+المؤسسات سواء داخل الجامعة أو خارج الجامعة لابد أن
+
+68
+00:06:02,380 --> 00:06:07,210
+يقدم شهادة بذلكهذا قد يُطلب من كلنا ومن الطلاب ..
+
+69
+00:06:07,210 --> 00:06:11,770
+من الطلاب ككل والطالبات في العام القادم إن شاء
+
+70
+00:06:11,770 --> 00:06:17,450
+الله غير التدريب، في فرق، غير التدريبالتدريب هو
+
+71
+00:06:17,450 --> 00:06:22,510
+عبارة عن ساعات أكاديمية، ساعات موجودة في المنهج
+
+72
+00:06:22,510 --> 00:06:27,370
+وفي المساق الأكاديمي كمساقات تدريبية، هذه الطالب
+
+73
+00:06:27,370 --> 00:06:30,650
+مكلف بها وخاصة في الخدمة الاجتماعية عندنا، عندنا
+
+74
+00:06:30,650 --> 00:06:34,510
+مساقات تدريبية في الفصل الأول و الفصل الثاني، ربما
+
+75
+00:06:34,510 --> 00:06:38,530
+في الخطة الجديدة نعمل أربع مساقات تدريبية على مدى
+
+76
+00:06:38,530 --> 00:06:39,590
+أربعة فصول
+
+77
+00:06:42,280 --> 00:06:49,320
+طيب إذا هنا بعض الخدمة العامة قد تكون أيضا بمقابل
+
+78
+00:06:49,320 --> 00:06:55,500
+بسيط بالنسبة لمن يقدم خدمة مقابل يعني أشياء بسيطة
+
+79
+00:06:55,500 --> 00:07:01,460
+مقابل الجهد الذي يبدو لهطبعا في لمحة تاريخية الآن
+
+80
+00:07:01,460 --> 00:07:05,240
+هناخدها عن التطوع، عن التطوع و هو التطور، لمحة
+
+81
+00:07:05,240 --> 00:07:11,040
+تاريخية، طبعا التطوع يعني موجود منذ وجود الإنسان
+
+82
+00:07:11,040 --> 00:07:15,220
+على هذه الأرض، و هو مساعدة الإنسان لأخيه الإنسان
+
+83
+00:07:16,130 --> 00:07:22,690
+منذ أن خلق الله الإنسان آدم وحواء وبعده الجماعات
+
+84
+00:07:22,690 --> 00:07:27,150
+البشرية الأخرى وجدت التطوع عن طريق المساعدة تقديم
+
+85
+00:07:27,150 --> 00:07:31,730
+المساعدة نعم فطرة يقدم المساعدة ولذلك يعني وجدت
+
+86
+00:07:31,730 --> 00:07:37,270
+الجماعات حول بعضها البعض من أجل التعاون من أجل
+
+87
+00:07:37,270 --> 00:07:43,730
+ابتعاد عن العزلة وهكذا فوجد هذا التطوعوهو مساعدة
+
+88
+00:07:43,730 --> 00:07:48,510
+الإنسان لأخيه الإنسان عن طريق التعاون ومقابلة
+
+89
+00:07:48,510 --> 00:07:54,490
+الحياةيعني مقابلة الوحوش، مقابلة الحياة القاسية،
+
+90
+00:07:54,490 --> 00:07:58,070
+مواجهة الجماعات الأخرى المعتادية على بعضها البعض،
+
+91
+00:07:58,070 --> 00:08:02,150
+هنا بيحدث اجتماع للإنسان مع أخيه الإنسان لمواجهة
+
+92
+00:08:02,150 --> 00:08:05,850
+هذه الأخطار، سواء كانت أخطار طبيعية أو أخطار
+
+93
+00:08:05,850 --> 00:08:10,590
+بشرية، يعني لابد من تعاون الإنسان مع أخيه الإنسان،
+
+94
+00:08:10,590 --> 00:08:15,050
+وهنا التعاون يكون بلا مقابلاجتماع الإنسان مع بعض
+
+95
+00:08:15,050 --> 00:08:18,610
+.. مع الإنسان و تعاون الإنسان مع الإنسان من أجل
+
+96
+00:08:18,610 --> 00:08:22,870
+مواجهة الأخطار الطبيعية و الأخطار الإنسانية و
+
+97
+00:08:22,870 --> 00:08:27,710
+الأخطار من خلال الوحوش التي قد يتعرضون لها أيضا،
+
+98
+00:08:27,710 --> 00:08:32,350
+واضح؟ فلذلك دائما بدأ الإنسان يجتمع، الإنسان يجتمع
+
+99
+00:08:32,350 --> 00:08:37,490
+مع أخيه الإنسان و هناك نشأت القرى و نشأت بعد ذلك
+
+100
+00:08:37,490 --> 00:08:42,040
+المدناللي هو مع بعضها البعض، لا نجد إنسان يعيش
+
+101
+00:08:42,040 --> 00:08:46,500
+لوحده بعيدا عن أخيه الإنسان، لا يمكن، لابد أن يكون
+
+102
+00:08:46,500 --> 00:08:51,660
+هناك تجمعات إنسانية تعيش مع بعضها البعض لمهاجرة
+
+103
+00:08:51,660 --> 00:08:59,960
+الأخطار أيضا الإسلام هنا دعي إلى التعاوندعا إلى
+
+104
+00:08:59,960 --> 00:09:03,780
+التعاون الأديان كلها دعت إلى التعاون وإلى التطوع
+
+105
+00:09:03,780 --> 00:09:08,340
+ولذلك من هنا الأديان كلها تحث على مساعدة الإنسان
+
+106
+00:09:08,340 --> 00:09:11,720
+لأخيه الإنسان لأنها كلها أديان ربانية من عند الله
+
+107
+00:09:11,720 --> 00:09:15,320
+سبحانه وتعالى آخر هذه الأديان اللي هو الإسلام
+
+108
+00:09:15,320 --> 00:09:19,200
+فالإسلام يدعو إلى مساعدة الإنسان وتعاون الإنسان مع
+
+109
+00:09:19,200 --> 00:09:23,120
+أخيه الإنسان وهذا واضح في قوله تعالى وتعاونوا على
+
+110
+00:09:23,120 --> 00:09:28,600
+البر والتقوى ولا تعاونوا على الإثم والعدوانفلذلك
+
+111
+00:09:28,600 --> 00:09:34,260
+من هنا لابد يعني دائما الإسلام يدعونا إلى عمل
+
+112
+00:09:34,260 --> 00:09:39,860
+الخير و إلى سواء من أجل الدنيا أو من أجل الآخرة و
+
+113
+00:09:39,860 --> 00:09:45,200
+هذا واضح في قوله تعالى يدعو الجميع بين الجمع بين
+
+114
+00:09:45,200 --> 00:09:49,480
+الجزاء الدنيا و الأخروي يقول الله تعالى وابتغي
+
+115
+00:09:49,480 --> 00:09:54,380
+فيما أتاك الله الدار الأخرى ولا تنسى نصيبك من
+
+116
+00:09:54,380 --> 00:10:01,510
+الدنيا وأحسن كما أحسن الله إليكولا تبغي الفسادة في
+
+117
+00:10:01,510 --> 00:10:06,510
+الأرض، إن الله لا يحب المفسدين، الله ما بيحب
+
+118
+00:10:06,510 --> 00:10:11,150
+المفسدين ونحن لا نحب المفسدين، الفساد قافة
+
+119
+00:10:11,150 --> 00:10:17,250
+والمفسدين أيضا قافة في المجتمع، فلذلك يعني .. و
+
+120
+00:10:17,250 --> 00:10:22,630
+الإسلام هنا يعني يحطن على العمل، يعني في الدنيا و
+
+121
+00:10:22,630 --> 00:10:24,570
+أيضا للآخرة
+
+122
+00:10:26,170 --> 00:10:33,110
+فلذلك من هنا لابد من التطوع التطوع أحيانا يكون من
+
+123
+00:10:33,110 --> 00:10:40,030
+أجل كسب السواب من الله سبحانه وتعالىهذا واضح يعني
+
+124
+00:10:40,030 --> 00:10:44,750
+أيضا في الحديث اعمل لدنياك كأنك تعيش أبدا و اعمل
+
+125
+00:10:44,750 --> 00:10:50,330
+لآخرتك كأنك تموت غدا فلذلك من هنا حدثنا الإسلام
+
+126
+00:10:50,330 --> 00:10:56,150
+على العمل الدنيوي و العمل أيضا من أجل الآخرة فلابد
+
+127
+00:10:56,150 --> 00:10:59,770
+أن نعمل من أجل الدنيا و من أجل الآخرة
+
+128
+00:11:02,870 --> 00:11:06,850
+أيضا الرسول صلى الله عليه و سلم هذا واضح في القول
+
+129
+00:11:06,850 --> 00:11:11,310
+اعمل الدنيا كأنك تعيش أبدا و اعمل آخرتك كأنك تموت
+
+130
+00:11:11,310 --> 00:11:16,870
+غدا فلذلك لابد من العمل للدنيا و للآخرة و هذه طبعا
+
+131
+00:11:16,870 --> 00:11:21,870
+فيها حس على فعل الخير و العمل التطوعي و حس الشباب
+
+132
+00:11:21,870 --> 00:11:25,790
+على التطوع و القيام بأعمال التطوع و أعمال الخير
+
+133
+00:11:25,790 --> 00:11:31,870
+هذه كلها ديننا الإسلامي يحسنا على ذلكطبعا الإسلام
+
+134
+00:11:31,870 --> 00:11:36,470
+بهذه الصورة يؤكد على أمران طبيعيين تدفع عليهم
+
+135
+00:11:36,470 --> 00:11:41,690
+طبيعة الإنسان ووجوده الخاص ما هما أولا أن يعيش في
+
+136
+00:11:41,690 --> 00:11:45,130
+الحياة ليحفظ نفسه الإنسان يعيش في الحياة ليحفظ
+
+137
+00:11:45,130 --> 00:11:49,830
+لنفسه البقاء نحن نعيش في هذه الحياة من أجل إيه أن
+
+138
+00:11:49,830 --> 00:11:54,570
+نحفظ لأنفسنا البقاء النحية الثانية لا نعيش لأنفسنا
+
+139
+00:11:54,570 --> 00:11:59,130
+فقط من أجل الحياة لابد من مشاركة الآخرين أن
+
+140
+00:11:59,130 --> 00:12:04,130
+نشاركهمولذلك الإنسان كائن اجتماعي بطبيعي فلذلك هنا
+
+141
+00:12:04,130 --> 00:12:07,910
+لا يعيش لنفس الإنسان فقط ولكن لابد من مشاركته
+
+142
+00:12:07,910 --> 00:12:14,470
+للآخرين المشاركة للآخرين في كل النشاطات النشاطات
+
+143
+00:12:14,470 --> 00:12:19,270
+المختلفة المحققة لصالح من الفرد والجماعة والمجتمع
+
+144
+00:12:19,270 --> 00:12:24,790
+فلذلك من هنا لابد من المشاركةلا يعيش الإنسان لنفسه
+
+145
+00:12:24,790 --> 00:12:29,350
+فقط بل أيضا يعيش للآخرين ويشارك الآخرين من أجل
+
+146
+00:12:29,350 --> 00:12:34,450
+تحقيق المصلحة، مصلحة الفرد والجماعة والمجتمع وهذا
+
+147
+00:12:34,450 --> 00:12:39,970
+الإسلام يحثنا على ذلك طبعا
+
+148
+00:12:40,640 --> 00:12:44,220
+لابد من الملاقة ما بين أن يعيش الإنسان لنفسه وأن
+
+149
+00:12:44,220 --> 00:12:47,500
+يعيش أيضا من أجل الآخرين فلذلك من هنا لا يعيش
+
+150
+00:12:47,500 --> 00:12:52,040
+الإنسان في عزلة وإنما لابد من مشاركته للآخرين من
+
+151
+00:12:52,040 --> 00:12:56,120
+أجل بناء المجتمع ومن أجل تحقيق المصلحة الفردية
+
+152
+00:12:56,120 --> 00:13:01,240
+والجماعية والمجتمعية طبعا
+
+153
+00:13:08,840 --> 00:13:13,560
+فكرة هذه إنه يعيش الإنسان مع الآخرين هنا بنحقق
+
+154
+00:13:13,560 --> 00:13:16,700
+مبدأ مهم جدا يدعو إليه الإسلام اللي هو مبدأ
+
+155
+00:13:16,700 --> 00:13:22,870
+التكافل الاجتماعيمبدأ التكافل الاجتماعي الغني لابد
+
+156
+00:13:22,870 --> 00:13:26,790
+أن يخرج حق الفقير في الذكاء والصداقات وغير ذلك
+
+157
+00:13:26,790 --> 00:13:32,570
+وكذلك الفقير لابد أيضا أن يعيش في هذا المجتمع
+
+158
+00:13:32,570 --> 00:13:37,190
+ويبني نفسه ويعتمد على نفسه وأيضا لابد أن يكون هناك
+
+159
+00:13:37,190 --> 00:13:43,540
+تكافل بينه وبين الغني كذلكالقوي أن يساعد الضعيف
+
+160
+00:13:43,540 --> 00:13:47,900
+وأن يكون هناك تكافل أيضًا ما بين الأقوياء والضعفاء
+
+161
+00:13:47,900 --> 00:13:53,240
+في المجتمع وما بين فئات المجتمع كلها فلذلك من هنا
+
+162
+00:13:53,240 --> 00:13:58,500
+هذا مبدأ التكافل مبدأ عام وهم جدًا في المجتمع
+
+163
+00:13:59,070 --> 00:14:02,910
+فالتعاون يعني وصلنا إلى التكافل الاجتماعي في
+
+164
+00:14:02,910 --> 00:14:06,490
+المجتمع ما بين الأخوياء والضعفاء، ما بين الأغنياء
+
+165
+00:14:06,490 --> 00:14:10,430
+والفقراء، أيضًا ما بين الكبير والصغير، أيضًا ما
+
+166
+00:14:10,430 --> 00:14:14,250
+بين التكافل بين أفراد الأسرة الواحدة، لابد أن يكون
+
+167
+00:14:14,250 --> 00:14:19,250
+هناك تكافل اجتماعي ما بين الأبناء وبعضهم البعض وما
+
+168
+00:14:19,250 --> 00:14:24,620
+بين الأب، فلذلك الأب يكفل أبناءهوهم صغار وكذلك
+
+169
+00:14:24,620 --> 00:14:29,100
+الأبناء عندما يكبرون واحتاج الأب أو الأم للأبناء
+
+170
+00:14:29,100 --> 00:14:33,060
+لابد أن يكفلونا من رعاية من الأباء والامهات فهنا
+
+171
+00:14:33,060 --> 00:14:37,740
+بيحدث عندنا يعني و هكذا في المجتمع وإذا لم يكفل
+
+172
+00:14:37,740 --> 00:14:42,840
+هؤلاء فالمجتمع قادر على أن يكفلهم جميعا و هكذا
+
+173
+00:14:49,500 --> 00:14:54,660
+في عندنا من ناحية تطور و تطوع طبعا تطور هذا التطوع
+
+174
+00:14:54,660 --> 00:14:59,460
+منذ كما قلنا من بداية المحاضرة منذ الخليقة و بدأ
+
+175
+00:14:59,460 --> 00:15:05,420
+الإنسان يفكر بالاجتماع مع أخيه الإنسان و أبدأ أيضا
+
+176
+00:15:05,420 --> 00:15:10,980
+في الخدمات التي تقدم لم تأخذ الشكل المنظم في الدول
+
+177
+00:15:10,980 --> 00:15:16,270
+الغربية لكن بعد ذلكيعني أدرك الناس أن عدم كفاية
+
+178
+00:15:16,270 --> 00:15:19,890
+الخدمات اللي كانت تقدم لهم من خلال المؤسسات
+
+179
+00:15:19,890 --> 00:15:24,970
+المختلفة يعني خاصة كان قانون الفقر هذا في بريطانيا
+
+180
+00:15:24,970 --> 00:15:29,350
+فأحيانا الخدمات تكفي أو لا تكفي فلذلك إذا لم تكفي
+
+181
+00:15:29,350 --> 00:15:39,570
+الخدمات فنحن نحتاج إلى خدمات المتطوعين طيب
+
+182
+00:15:46,830 --> 00:15:51,660
+طبعا احنا تعرفين جمعيات تنظيم الإحسانهذه الجمعيات
+
+183
+00:15:51,660 --> 00:15:55,140
+تنظيم الإحسان بتقدم الخدمات للفقراء والمحتاجين
+
+184
+00:15:55,140 --> 00:15:59,420
+وهذه نشأت في أوروبا وفي أمريكا فلذلك هذه كانت تقوم
+
+185
+00:15:59,420 --> 00:16:05,260
+على التطوع التطوع من من؟ من الأغنياء لهذه المؤسسات
+
+186
+00:16:05,260 --> 00:16:10,320
+وهذه المؤسسات كانت تنظم الفقراء وتقوم بإعطائهم من
+
+187
+00:16:10,320 --> 00:16:14,400
+اللي هم للفقراء اللي هي المساعدات للفقراء فلذلك
+
+188
+00:16:14,400 --> 00:16:17,620
+جمعيات تنظيم الإحسان كانت حلقة الوصل ما بين
+
+189
+00:16:17,620 --> 00:16:22,810
+الأغنياء وما بين الفقراءفهذه يعتبر نوع من اللي هو
+
+190
+00:16:22,810 --> 00:16:28,530
+الجمعيات الاجتماعية اللي كانت تقدم خدمات في أوروبا
+
+191
+00:16:28,530 --> 00:16:35,030
+وفي أمريكا للفقراء ولمحتاجين أيضا
+
+192
+00:16:38,110 --> 00:16:44,730
+التطوع أيضًا بدأ ياخذ شكل جماعي وليس شكل فردي بحيث
+
+193
+00:16:44,730 --> 00:16:49,170
+أن الجهود الفردية لا تكفي لمواجهة الحاجات المتنوعة
+
+194
+00:16:49,170 --> 00:16:53,290
+فالمشكلات المتعددة والمتجددة أخذت في التراكم مع
+
+195
+00:16:53,290 --> 00:16:57,010
+بداية الثورة الصناعية واحنا عارفين أن الثورة
+
+196
+00:16:57,010 --> 00:17:01,790
+الصناعية وجود الآلة ولذلك كانوا يعيشون قبل ثورة
+
+197
+00:17:01,790 --> 00:17:06,420
+الصناعية في المجتمع الزراعيوالمجتمع الزراعي العمال
+
+198
+00:17:06,420 --> 00:17:09,540
+أو القطاعين الذين يملكون الأراضي الزراعية لم تصلح
+
+199
+00:17:09,540 --> 00:17:12,720
+معهم الأراضي فلذلك انتقلوا مع التورة الصناعية إلى
+
+200
+00:17:12,720 --> 00:17:17,680
+المدن من الريف إلى المدن فصارت هناك هجرة فهناك
+
+201
+00:17:17,680 --> 00:17:22,760
+شروا الآلات والمصانع وبدأ العمال يهاجرون من الريف
+
+202
+00:17:22,760 --> 00:17:27,280
+إلى المصانع اللي هي في المدينة وتولدت مشكلات هذه
+
+203
+00:17:27,280 --> 00:17:33,300
+المشكلات كلها تحتاج إلى تطوح لعلاجهامشكلات المرأة
+
+204
+00:17:33,300 --> 00:17:38,020
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+243
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+245
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+246
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+الأجهزة المختلفة
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+247
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+248
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+249
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+250
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+برزوا كيعني له و لهم دور بارز في طريق تنظيم
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+251
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+252
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+253
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+254
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+255
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+256
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+الخدمة الاجتماعية ولذلك مهنة الخدمة الاجتماعية
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+257
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+258
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+المختلفةبدنا نصل الآن إلى الناحية المهنية مدارس
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+259
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+260
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+تقدمت الخدمة الاجتماعية نظرا لتقدم العلوم
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+261
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+262
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+فلذلك أصبحت الحاجة ماسة لاستخدام مهنيين في تقديم
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+263
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+264
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+الاجتماعيةفعندنا ظهرت مدارس الخدمة الاجتماعية من
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+265
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+خلال جمعيات تنظيم الإحسان وفي عندنا كانت جمعية
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+266
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+نيويورك لتنظيم الإحسان أول من أسسوا مدرسة لتدريب
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+267
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+من العاملين في مجالات الرعاية الاجتماعيةفي عام
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+268
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+1898 اللي هي اليوم أصبحت يطلق عليها مدرسة الخدمة
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+269
+00:22:48,090 --> 00:22:52,390
+الاجتماعية في كولومبيا في كولومبيا في أمريكا هذه
+
+270
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+كولومبيا جامعة و مدرسة كان يطلق عليها مدرسة الخدمة
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+271
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+
+272
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+طبعا اللاخصائيين يعني المتطوعون ومؤسسات خدمة الفرد
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+273
+00:23:10,420 --> 00:23:16,840
+طبعا تخرج الرعيل الأول من اللاخصائيين الاجتماعيين
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+274
+00:23:16,840 --> 00:23:21,640
+بدأوا دور المتطوعين يتضاءل خاصة في مجال خدمة الفرد
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+275
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+لأن هنا المدارس الخدمة الاجتماعية خرجت لنا يعني
+
+276
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+انتوا عارفين ميرا ريتشموند عام 1917 اللي هي دعت أو
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+277
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+أنشط اللي هي خدمة الفردوبدأ الأهل الأخصائيين
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+278
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+يتعلمون وأول ما ظهرت هي طريقة خدمة الفرد فالموظفين
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+279
+00:23:40,370 --> 00:23:44,270
+أو العاملين المهنيين في خدمة الفرد لم يحتاجوا
+
+280
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+المتطوعين بكثرةفلذلك يعني لأنه كان فيه عدد كبير
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+منهم يعني متخصصين في خدمة الفرد فلذلك كان هناك
+
+282
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+يعني المتطوعين بالنسبة في مجال خدمة الفرد قليل جدا
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+لماذا؟ لأنه كان عدد الخريجين في خدمة الفرد عدد
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+284
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+كبير جدا فلذلك لم يستعينوا بهم إلا القليل بعد ذلك
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+285
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+في خدمة الجماعة يعني خدمة الجماعةطبعا لماذا طيب
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+286
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+حرص اللخصيين على تأكيد وجودهم فلذلك لم يكن لهم دور
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+287
+00:24:20,770 --> 00:24:23,810
+في خدمة الفرد دور كبير ليه؟ لأن اللخصيين
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+288
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+الاجتماعيين كانوا دائما يبدلوا أقصى ما يملكون فيه
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+289
+00:24:27,830 --> 00:24:33,590
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+290
+00:24:33,590 --> 00:24:36,990
+من منافس المتطوعين لهم أو تدخلهم في عملهم المهني
+
+291
+00:24:36,990 --> 00:24:41,710
+فلذلك كان الإقبال على المتطوعين قليل في طريقة خدمة
+
+292
+00:24:41,710 --> 00:24:46,250
+الفننجي نشوف بعد ذلك خدمة الجماعة في مؤسسات خدمة
+
+293
+00:24:46,250 --> 00:24:49,930
+الجماعة هناك إقبال و هناك رغبة في المتطوعين
+
+294
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+لمساعدة الأخصائي الاجتماعيين في العمل المؤسسي من
+
+295
+00:24:54,290 --> 00:24:58,910
+خلال مؤسسات خدمة الجماعة فلذلك هناك كان إقبال من
+
+296
+00:24:58,910 --> 00:25:04,640
+المتطوعين على مؤسسات خدمة الجماعةطبعا ليه؟ لأنه
+
+297
+00:25:04,640 --> 00:25:08,400
+كان فيه نقص في المهنيين في خدمة الجماعة فلذلك
+
+298
+00:25:08,400 --> 00:25:13,240
+كانوا يستعينونا الاخصائين الاجتماعيين بالمتطوعين
+
+299
+00:25:13,240 --> 00:25:17,240
+بالمتطوعين في اللي هي خدمة الجماعة والعمل مع
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+300
+00:25:17,240 --> 00:25:23,100
+الجماعات المختلفةأيضا كان المتطابقين لهم دور في
+
+301
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+مجالس الإدارة، مجالس إدارة المؤسسات الاجتماعية،
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+لما لهم تأثير في هذه المؤسسات من خلال مجالس
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+303
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+الإدارةنجي تشوف المتطوعين في تنظيم المجتمع في طريق
+
+304
+00:25:37,740 --> 00:25:41,940
+تنظيم المجتمع كان لهم دور بارز لماذا؟ لأن طريق
+
+305
+00:25:41,940 --> 00:25:46,060
+تنظيم المجتمع تعتمد على المشاركة الشعبية تعتمد على
+
+306
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+المشاركة عند مواجهة المشكلات ودراسة المشكلات فلذلك
+
+307
+00:25:50,600 --> 00:25:54,540
+في طريق تنظيم المجتمع يعني الخدمة الاجتماعية
+
+308
+00:25:54,540 --> 00:25:58,400
+استعانت بالمتطوعين من أبناء المجتمع لماذا؟ لأن
+
+309
+00:25:58,400 --> 00:26:02,510
+أبناء المجتمع هم الأقدر على فهم مشكلتهموطريقة
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+310
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+تنظيم المجتمع تقوم بحل المشكلات عن طريق المشاركة
+
+311
+00:26:06,570 --> 00:26:11,410
+مشاركة أبناء المجتمع في حل المشكلات فلذلك كان هناك
+
+312
+00:26:11,410 --> 00:26:17,210
+إقبال من خلال المتطوعين من أبناء المجتمع ومشاركة
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+313
+00:26:17,210 --> 00:26:21,850
+عن طريق المشاركة في مؤسسات تنظيم المجتمعفلذلك
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+314
+00:26:21,850 --> 00:26:25,850
+طريقة تنظيم المجتمع ما زالت إلى اليوم في حاجة ماسة
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+315
+00:26:25,850 --> 00:26:30,610
+إلى المتطوعين للعمل في مختلف مؤسسات الرعاية
+
+316
+00:26:30,610 --> 00:26:34,410
+الاجتماعية التي تعمل فيها طريقة تنظيم المجتمع
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+317
+00:26:42,280 --> 00:26:46,560
+طبعا في الوقت الحالي احنا يعني المطابعين اللي يعني
+
+318
+00:26:46,560 --> 00:26:51,800
+لهم دور كبير ونحتاجهم ولهم دور بارز والمجتمعات
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+319
+00:26:51,800 --> 00:26:56,260
+كلها سواء كانت غنية او فقيرة نامية او متقدمة كلها
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+320
+00:26:56,260 --> 00:27:00,100
+تحتاج إلى العمل التطوعي فاليوم نحن في أمس الحاجة
+
+321
+00:27:00,100 --> 00:27:04,580
+إلى العمل التطوعي في مؤسساتنا الاجتماعية من أجل
+
+322
+00:27:04,580 --> 00:27:09,940
+مساعدة المجتمع على مواجهة مشكلته فهم المشكلات
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+323
+00:27:10,680 --> 00:27:15,200
+دراستها دراسة علمية وبعد ذلك القيام بوضع البرامج
+
+324
+00:27:15,200 --> 00:27:20,620
+والمشاريع اللازمة بناء على خطط معينة وتخطيط قبل
+
+325
+00:27:20,620 --> 00:27:26,620
+ذلك من أجل مواجهة هذه المشكلات والتغلب عليها فلذلك
+
+326
+00:27:26,620 --> 00:27:31,760
+نحن نحتاج إلى طاقات المتطوعون من الذكوري والإينات
+
+327
+00:27:31,760 --> 00:27:35,840
+في مؤسساتنا الاجتماعية ونحن في مجتمعنا الفلسطيني
+
+328
+00:27:36,210 --> 00:27:41,650
+أيضا نحتاج إلى المتطابعين من الذكور والإناث في
+
+329
+00:27:41,650 --> 00:27:45,770
+كافة التخصصات سواء في الخدمة الاجتماعية أو في
+
+330
+00:27:45,770 --> 00:27:51,710
+المؤهلات الأخرى من هندسة وطب و تربية وغير ذلك
+
+331
+00:27:56,330 --> 00:27:59,470
+والحاجة اليوم للمتطوعين في كل مجالات الخدمة
+
+332
+00:27:59,470 --> 00:28:03,930
+الاجتماعية تزداد كلما تسعت المجالات كلما تسعت
+
+333
+00:28:03,930 --> 00:28:07,550
+مجالات الخدمة الاجتماعية تزداد الحاجة إلى
+
+334
+00:28:07,550 --> 00:28:12,250
+المتطوعين لماذا الحاجة للمتطوعين في مجالات الخدمة
+
+335
+00:28:12,250 --> 00:28:16,270
+الاجتماعية تزداد؟ لماذا؟ لأسباب معينة، كل طالبة
+
+336
+00:28:16,270 --> 00:28:20,130
+أطلب منها أن تذكرلي سبب معين، أيوة يا بنتي الوصول
+
+337
+00:28:20,130 --> 00:28:23,450
+لدفع الإبنانيين في مجلس الاستثمار ومجلس التطوعين
+
+338
+00:28:25,960 --> 00:28:30,200
+بالضبط، إذا أول سبب، نحن في حاجة للمتطوعين في
+
+339
+00:28:30,200 --> 00:28:35,200
+الخدمة الاجتماعية لوجود النقص في المهنيين وهذا
+
+340
+00:28:35,200 --> 00:28:39,200
+بيستدعي استكمال النقص من خلال اللي هو المتطوعين
+
+341
+00:28:39,200 --> 00:28:43,160
+السبب الثاني، حاجتنا للمتطوعين، أيوة يا بنتك
+
+342
+00:28:43,160 --> 00:28:46,720
+المتطوع يعني برنامج بسيطة وطريقة ومبينية للمشكلات
+
+343
+00:28:46,720 --> 00:28:50,680
+الاجتماعية؟ لأ المتطوع دائما يحس بالمشكلات
+
+344
+00:28:51,810 --> 00:28:55,510
+الاجتماعية التي يعيش فيها ويكون أقدر على فهم
+
+345
+00:28:55,510 --> 00:29:00,290
+مشكلاته فلذلك نحن نحتاج لمتطوعين من أبناء المجتمع
+
+346
+00:29:00,290 --> 00:29:03,670
+الذي نعمل معه فالاقتصاد الاجتماعي يحتاج لهؤلاء
+
+347
+00:29:03,670 --> 00:29:08,710
+المتطوعين لفهمهم لمشكلاتهم والاحساس بها والاعتماد
+
+348
+00:29:08,710 --> 00:29:13,710
+عليهم في فهم هذه المشكلات والتعبير عنها فلذلك نحن
+
+349
+00:29:13,710 --> 00:29:18,710
+في أمثل حاجة إلى هؤلاء المتطوعين الذين اتعاملون مع
+
+350
+00:29:18,710 --> 00:29:25,240
+أبناء مجتمعهم ماذا أيضا؟أيوة بنتي أن المتطوع عندما
+
+351
+00:29:25,240 --> 00:29:28,820
+يندمش في مجالات العمل الإنساني سيلمس بنفسه مشكلات
+
+352
+00:29:28,820 --> 00:29:32,680
+المجتمع الذي ينتمي إليه وقد يدفعه ذلك إلى المساعدة
+
+353
+00:29:32,680 --> 00:29:36,680
+في التخيل من حدث المشكلة بالطب المتطوع هو يعني
+
+354
+00:29:36,680 --> 00:29:40,300
+اللي بيجبه من أبناء المجتمع كما قلت قبل قليل وأيضا
+
+355
+00:29:40,300 --> 00:29:44,000
+هو اللي بيجبه قادر على فهم مشكلته ومشكلة المجتمع
+
+356
+00:29:44,000 --> 00:29:48,120
+اللي يعيش فيه فلذلك من هنا نحن نستنعين بالمتطوعين
+
+357
+00:29:48,120 --> 00:29:54,840
+لأنهم يعنييفكرون في مشكلة إجتماعهم ويفكرون في
+
+358
+00:29:54,840 --> 00:29:59,020
+مشكلات مجتمعهم وكيفية مواجهة هذه المشكلات فنحن هنا
+
+359
+00:29:59,020 --> 00:30:06,580
+استعين بطاقات المتطوعين نعم ماذا أيضا؟طبعا التطوع
+
+360
+00:30:06,580 --> 00:30:10,620
+هذا بيعمل الانتماء كذلك يعني يزيد الانتماء لأنه
+
+361
+00:30:10,620 --> 00:30:14,680
+عندما نستعين بالمتطوعين نحن نعمل على زيادة انتماء
+
+362
+00:30:14,680 --> 00:30:19,560
+هؤلاء إلى مجتمعهم الانتماء وعندما نزيد الانتماء
+
+363
+00:30:19,560 --> 00:30:23,900
+لدى المتطوع تزيد المشاركة فلذلك في علاقة ترضية ما
+
+364
+00:30:23,900 --> 00:30:28,180
+بين المشاركة والانتماء للمجتمع فمن خلال التطوع
+
+365
+00:30:28,180 --> 00:30:32,740
+نعمل على زيادة الانتماء ونعمل على المشاركة زيادة
+
+366
+00:30:32,740 --> 00:30:38,330
+المشاركة واضح؟طيب، ماذا أيضًا للأسباب الأخرى؟ آه
+
+367
+00:30:38,330 --> 00:30:48,510
+.. أيه يا بنتي؟ مجالات
+
+368
+00:30:48,510 --> 00:30:53,330
+جديدة وخاصة نحن في العصر ممكن أن يكون هناك مجالات
+
+369
+00:30:53,330 --> 00:30:57,050
+جديدة وكل ما كانت مجالات جديدة نحن نكون في حاجة
+
+370
+00:30:57,050 --> 00:31:03,670
+إلى متطوعين جدد متطوعين إيه؟ جدد، نعم، ماذا أيضًا؟
+
+371
+00:31:04,920 --> 00:31:08,680
+أه يا بنتى نعم ان تهيأ .. تهيأ المنافسة و الممارسة
+
+372
+00:31:08,680 --> 00:31:10,380
+.. الممارسة بالعمل في الخدمة الاجتماعية في
+
+373
+00:31:10,380 --> 00:31:13,660
+الاجتماعات المختلفة تعتبر من أعزف الأمور التي بؤثر
+
+374
+00:31:13,660 --> 00:31:17,000
+على نجاح المهنة في تحقيقها هدفها يمكن المتطوع .. و
+
+375
+00:31:17,000 --> 00:31:20,200
+يمكن المتطوعين أن يساعدوا و يماركدوا في خلق هذا
+
+376
+00:31:20,200 --> 00:31:25,880
+المنافسمن هنا نستعين
+
+377
+00:31:25,880 --> 00:31:30,860
+بالمتطوعين بالتعامل من أجل شرح مهنة الخدمة
+
+378
+00:31:30,860 --> 00:31:35,040
+الاجتماعية وأيضا نستعين بهم لتوصيل المعلومات من
+
+379
+00:31:35,040 --> 00:31:37,400
+خلال الخصائص الاجتماعيين عن طريق المتطوعين إلى
+
+380
+00:31:37,400 --> 00:31:41,580
+المجتمع الذي يعيشون فيه فلذلك بجبه هم حلقة الوصل
+
+381
+00:31:41,580 --> 00:31:45,500
+ما بين الخصائص الاجتماعي وما بين المجتمع الذي
+
+382
+00:31:45,500 --> 00:31:50,250
+يعيشون فيه فلذلك من هنا نستعين بالمتطوعينماذا
+
+383
+00:31:50,250 --> 00:31:55,950
+أيضا؟ نعم طيب
+
+384
+00:31:55,950 --> 00:32:00,890
+يعني إلى هنا نعم في أي سؤال في موضوعنا اليوم عن
+
+385
+00:32:00,890 --> 00:32:07,070
+تطور التطوع و فهم اللي هو التطوع و كيف اليوم نحن
+
+386
+00:32:07,070 --> 00:32:11,030
+في أمثل حاجة إلى المتطوعين للعمل في مجالات الخدمة
+
+387
+00:32:11,030 --> 00:32:13,410
+الاجتماعية المختلفة
+
+388
+00:32:17,890 --> 00:32:23,390
+طبعا إلى هنا ننهي هذه المحاضرة على أمل أن نلتقي
+
+389
+00:32:23,390 --> 00:32:27,230
+بكلنا ان شاء الله في المحاضرة القادمة لنعقب عما
+
+390
+00:32:27,230 --> 00:32:33,210
+سبق ما أخذناه وأيضا ناخد موضوع من هو المتطوع
+
+391
+00:32:33,210 --> 00:32:38,250
+ومحاصفات المتطوع إلى أن نلتقي بكلنا ان شاء الله في
+
+392
+00:32:38,250 --> 00:32:43,190
+المحاضرة القادمة لكنا مني كل تحية وتقدير والسلام
+
+393
+00:32:43,190 --> 00:32:45,230
+عليكم ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/-CoHtax7Ikg_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/-CoHtax7Ikg_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..18baab117a45eef31594ee375e3cc3be290021b1
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/-CoHtax7Ikg_postprocess.srt
@@ -0,0 +1,12 @@
+1
+00:00:19,710 --> 00:00:23,810
+السلام عليكم ورحمة الله وبركاته نستقبل معكم سلسلة
+
+2
+00:00:23,810 --> 00:00:27,990
+Microbiologia الجامعة الإسلامية فيديو اليوم بانواع
+
+3
+00:00:27,990 --> 00:00:29,170
+كبسول الثاني
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/-CoHtax7Ikg_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/-CoHtax7Ikg_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..62ad956a7b4f80857fef67961fa36ef446bd96f0
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/-CoHtax7Ikg_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 2917, "start": 19.71, "end": 29.17, "text": "السلام عليكم ورحمة الله وبركاته نستقبل معكم سلسلة Microbiologia الجامعة الإسلامية فيديو اليوم بانواع كبسول الثاني", "tokens": [6027, 3794, 37440, 25894, 24793, 4032, 2288, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224, 8717, 14851, 4587, 36150, 20449, 24793, 8608, 1211, 3794, 37977, 5818, 20027, 24103, 25724, 10943, 27884, 33688, 3794, 37440, 10632, 8978, 16254, 2407, 45595, 20498, 4724, 7649, 14407, 3615, 9122, 3555, 3794, 12610, 6024, 104, 7649, 1829], "avg_logprob": -0.21889740003729766, "compression_ratio": 1.4142857142857144, "no_speech_prob": 2.562999725341797e-06, "words": [{"start": 19.71, "end": 19.89, "word": "السلام", "probability": 0.6573079427083334}, {"start": 19.89, "end": 20.27, "word": " عليكم", "probability": 0.96533203125}, {"start": 20.27, "end": 20.75, "word": " ورحمة", "probability": 0.9154052734375}, {"start": 20.75, "end": 21.01, "word": " الله", "probability": 0.9755859375}, {"start": 21.01, "end": 21.81, "word": " وبركاته", "probability": 0.9923828125}, {"start": 21.81, "end": 22.55, "word": " نستقبل", "probability": 0.61724853515625}, {"start": 22.55, "end": 23.13, "word": " معكم", "probability": 0.7802734375}, {"start": 23.13, "end": 23.81, "word": " سلسلة", "probability": 0.9613037109375}, {"start": 23.81, "end": 24.69, "word": " Microbiologia", "probability": 0.5889485677083334}, {"start": 24.69, "end": 25.17, "word": " الجامعة", "probability": 0.9562174479166666}, {"start": 25.17, "end": 25.95, "word": " الإسلامية", "probability": 0.973876953125}, {"start": 25.95, "end": 26.65, "word": " فيديو", "probability": 0.8753255208333334}, {"start": 26.65, "end": 27.03, "word": " اليوم", "probability": 0.918212890625}, {"start": 27.03, "end": 27.99, "word": " بانواع", "probability": 0.8067626953125}, {"start": 27.99, "end": 28.61, "word": " كبسول", "probability": 0.7252197265625}, {"start": 28.61, "end": 29.17, "word": " الثاني", "probability": 0.787353515625}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 154.36625, "duration_after_vad": 9.666875000000005}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/-CoHtax7Ikg_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/-CoHtax7Ikg_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..18baab117a45eef31594ee375e3cc3be290021b1
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/-CoHtax7Ikg_raw.srt
@@ -0,0 +1,12 @@
+1
+00:00:19,710 --> 00:00:23,810
+السلام عليكم ورحمة الله وبركاته نستقبل معكم سلسلة
+
+2
+00:00:23,810 --> 00:00:27,990
+Microbiologia الجامعة الإسلامية فيديو اليوم بانواع
+
+3
+00:00:27,990 --> 00:00:29,170
+كبسول الثاني
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/4-SUdsxRvPI_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/4-SUdsxRvPI_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..2889675ff7eba68f5619b5d823b636b964bee3c7
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/4-SUdsxRvPI_postprocess.srt
@@ -0,0 +1,1292 @@
+1
+00:00:01,480 --> 00:00:05,740
+Acid Fast Stain أو الإسم الآخر Zell-Nelson Stain
+
+2
+00:00:05,740 --> 00:00:10,360
+علمان اكتشفوا هذه الصبغة فصميت باسمها The Acid
+
+3
+00:00:10,360 --> 00:00:13,180
+Fast Stain is another differential staining method
+
+4
+00:00:13,180 --> 00:00:15,900
+اتفقنا إنه هي نوع من أنواع الـDifferential Stain
+
+5
+00:00:15,900 --> 00:00:19,900
+اللي هتقسم البكتيريا لمجموعة فهتقسم البكتيريا ل
+
+6
+00:00:19,900 --> 00:00:24,640
+Acid Fast بس اللهي وNon Acid Fast بس اللهي في
+
+7
+00:00:24,640 --> 00:00:27,540
+الإجرام الستان قلنا إنه مش كل البكتيريا بقدر
+
+8
+00:00:27,540 --> 00:00:30,770
+أصبغهافي عندي استثناء في عندي بكتيريا الـ
+
+9
+00:00:30,770 --> 00:00:34,750
+Mycobacterium هذه البكتيريا ما بقدر أصبغها بالـ
+
+10
+00:00:34,750 --> 00:00:38,610
+Gram Stain عشان هيك بستخدم ال Acid Fast Stain
+
+11
+00:00:38,610 --> 00:00:43,210
+لصيباغتها in this case the target cell are usually
+
+12
+00:00:43,210 --> 00:00:46,310
+members of the genus Mycobacterium ال
+
+13
+00:00:46,310 --> 00:00:49,430
+Mycobacterium هي اللي بستهدفها في ال Acid Fast
+
+14
+00:00:49,430 --> 00:00:53,430
+Stain طيب ليش أنا ماقدر أصبغ ال Mycobacterium بال
+
+15
+00:00:53,430 --> 00:00:57,380
+Gram Stain أو حتى بال symbol Stainبقول لي لأن هاي
+
+16
+00:00:57,380 --> 00:01:00,760
+البكتيريا ال cell wall تبعها في high concentration
+
+17
+00:01:00,760 --> 00:01:04,680
+of wax celibate ال wax celibate هاي بتكون كحاجز
+
+18
+00:01:04,680 --> 00:01:09,280
+بتمنع دخول الصبغة داخل البكتيريا وحتى لو أنا قدرت
+
+19
+00:01:09,280 --> 00:01:13,440
+أصبغها صعب أني أنا أزيل اللعب the cell walls of
+
+20
+00:01:13,440 --> 00:01:16,740
+these bacteria contain an unusually high
+
+21
+00:01:16,740 --> 00:01:19,660
+concentration of wax celibate the smoking
+
+22
+00:01:19,660 --> 00:01:22,440
+conventional symbol stand and the ground stand
+
+23
+00:01:22,440 --> 00:01:27,190
+uselessهذا بيجعل صعب صباغتها بالـ Gram Stain
+
+24
+00:01:27,190 --> 00:01:31,190
+والسمبل Stain The genus Mycobacterium الـ
+
+25
+00:01:31,190 --> 00:01:35,170
+Mycobacterium هو يعتبر genus content two important
+
+26
+00:01:35,170 --> 00:01:39,830
+human pathogen في عندي في هذه العائلة two species
+
+27
+00:01:39,830 --> 00:01:44,010
+مهمين بيسببوا مرض للإنسان الـ Mycobacterium
+
+28
+00:01:44,010 --> 00:01:47,870
+tuberculosis اللي بيسبب الصلق الرؤوي والـ
+
+29
+00:01:47,870 --> 00:01:52,430
+Mycobacterium liberi هاي اللي بتسبب الجذعهو مرض
+
+30
+00:01:52,430 --> 00:01:57,170
+بيكون فيه ندب بالجلد skin scar وبيصير فيه تدمير
+
+31
+00:01:57,170 --> 00:02:02,750
+بالجهاز العصبي brain damage ال M رمز لجيناس
+
+32
+00:02:02,750 --> 00:02:05,770
+Mycobacterium ال tuberculosis هي ال species
+
+33
+00:02:05,770 --> 00:02:10,570
+الكتابة العلمية للبكتيريا لازم ان انا احط خط او
+
+34
+00:02:10,570 --> 00:02:18,070
+انها تكون الكتابة مائلة هبدأ
+
+35
+00:02:18,070 --> 00:02:22,600
+بشرح ال procedure لان من خلالها هنتعرفكيف بدي أقدر
+
+36
+00:02:22,600 --> 00:02:27,420
+أصبغ هاي البكتيريا اللي صعب صباغتها أي استان في
+
+37
+00:02:27,420 --> 00:02:32,000
+معمل الـ Microbiology قولنا خطوات الصباغة، Smear
+
+38
+00:02:32,000 --> 00:02:35,960
+preparation، Air-drying، Heat-fixation، وستان،
+
+39
+00:02:35,960 --> 00:02:40,780
+استان بتختلف حسب نوع الصباغة هنتكلم على الـ Acid
+
+40
+00:02:40,780 --> 00:02:44,140
+-fast stain في الـ Gram stain الـ Primer stain
+
+41
+00:02:44,140 --> 00:02:47,600
+كانت لـ Crystal Violet لكن في الـ Acid-fast stain
+
+42
+00:02:47,600 --> 00:02:52,220
+هيختلف اسمها وهتختلف طريقة صباغتهاالـ Primer Stain
+
+43
+00:02:52,220 --> 00:02:56,180
+في الـ Acid-Fast Stain Carbolfoxin الـ Carbolfoxin
+
+44
+00:02:56,180 --> 00:02:59,820
+إذا متذكرين في معمل الـ Simplestain قلنا هي Basic
+
+45
+00:02:59,820 --> 00:03:04,460
+Stain لكن اللي بيميز الـ Carbolfoxin إنها Lipid
+
+46
+00:03:04,460 --> 00:03:07,800
+-Soluble والتي احنا قلنا إنه الـ Mycobacterium
+
+47
+00:03:07,800 --> 00:03:11,340
+بتحتوي على كمية كبيرة من الدهون عشان هيك ما بقدر
+
+48
+00:03:11,340 --> 00:03:15,520
+أصبغها بالSimplestain أو بالجرام Stain من خلال هذه
+
+49
+00:03:15,520 --> 00:03:21,440
+الخاصية هتساعد على اختراق الصبغة للـ Waxy Lipidلكن
+
+50
+00:03:21,440 --> 00:03:24,880
+بقولنا إن الاشي الرئيس اللي هيساعد على اختراق الـ
+
+51
+00:03:24,880 --> 00:03:29,980
+Carbolfoxin لـ Wax Libid هو الـ Steam شو يعني
+
+52
+00:03:29,980 --> 00:03:34,620
+Steam؟ الـ Steam هو البخار، عرض الصبغة لبخار ليش
+
+53
+00:03:34,620 --> 00:03:38,120
+عرض الصبغة لبخار؟ بقولّي البخار هيعمل الـ
+
+54
+00:03:38,120 --> 00:03:42,240
+Denaturation للروابط الكربونية الموجودة في الـ
+
+55
+00:03:42,240 --> 00:03:46,580
+Mycolic Acid اللي هو الـ Wax Libid بالتالي هيساعد
+
+56
+00:03:46,580 --> 00:03:52,100
+على اختراق الصبغة للـ Wax Libidهك انا اقدرت اصبغ
+
+57
+00:03:52,100 --> 00:03:55,340
+هذه البكتيريا من خلال استخدام الـ Primers تاني
+
+58
+00:03:55,340 --> 00:03:59,360
+الكربوفوكسين اللي هي Lipid Soluble الاشي الأساسي
+
+59
+00:03:59,360 --> 00:04:05,000
+من خلاله اقدرت اصبغ اللي هو تعريض البكتيريا لـ
+
+60
+00:04:05,000 --> 00:04:08,720
+Steam لبخار هيكسر الروابط الكربونية الموجودة في
+
+61
+00:04:08,720 --> 00:04:12,960
+الـ Mycolic Acid بالتالي هيساعد على اختراق الصبغة
+
+62
+00:04:12,960 --> 00:04:17,500
+للـ Wax Celibate كيف انا بدي اعمل هذه الخطوة بروح
+
+63
+00:04:17,500 --> 00:04:21,610
+بجيب بيكر بحط فيه ميهو بحطه على اللهب او الـ hot
+
+64
+00:04:21,610 --> 00:04:25,230
+plate لما تغلي الميه بروح بجيب ال slide اللي
+
+65
+00:04:25,230 --> 00:04:28,550
+عملتلها خطوة سمير preparation and dry heat
+
+66
+00:04:28,550 --> 00:04:32,270
+fixation و بحطها على طرف ال بيكر زي ما انتوا
+
+67
+00:04:32,270 --> 00:04:36,370
+شايفين بالصورة بغمر ال slide بالبراي مارستان
+
+68
+00:04:36,370 --> 00:04:40,950
+الكاربولفوكسين و بحسب 8 دقائق cover السمير with
+
+69
+00:04:40,950 --> 00:04:45,450
+carbolfoxin steam over boiling water for 8 minutes
+
+70
+00:04:45,450 --> 00:04:51,930
+بقول خلال 8 دقائقممكن ألاحظ أن أطراف ال slide جافة
+
+71
+00:04:51,930 --> 00:04:56,430
+أنه بدأت تتبخر الصبغة فبروح بضيف Carbol Fucsin
+
+72
+00:04:56,430 --> 00:05:00,950
+Added Additional Stain في Stain Was Off هان مهم
+
+73
+00:05:00,950 --> 00:05:04,810
+جدا أن أنا أضيف صبغة لأس لأنه لو جفت ال slide
+
+74
+00:05:04,810 --> 00:05:10,010
+هيتكون Insoluble Crystal هذه ال crystal راح يبين
+
+75
+00:05:10,010 --> 00:05:14,570
+شكلها بصلاي ولونها أحمر نفس لون الصبغة بالتالي
+
+76
+00:05:14,570 --> 00:05:18,630
+هتعطيني False Positive Resultلأن الـ Mycobacterium
+
+77
+00:05:18,630 --> 00:05:22,310
+في الـ Acid Fast Stain شكلها بصلاي هتظل محتفظة
+
+78
+00:05:22,310 --> 00:05:25,430
+بالـ Carbolfoxin يعني لونها أحمر هفكر عندي Acid
+
+79
+00:05:25,430 --> 00:05:30,210
+Fast بصلاي هو مافي عندي فقط نتيجة غلط في الشغل
+
+80
+00:05:30,210 --> 00:05:35,410
+الخطوة التانية بعد مرور 8 دقائق بقولي باطفل لها
+
+81
+00:05:35,410 --> 00:05:40,710
+وبستنقل عينها تبرد after slide has cooled ومهم جدا
+
+82
+00:05:40,710 --> 00:05:46,190
+ال cooling التبريدالتبريد هيعمل Renaturation إعادة
+
+83
+00:05:46,190 --> 00:05:50,570
+رابط الروابط الكربونية اللي اتكسرت بالتالي هتنحجز
+
+84
+00:05:50,570 --> 00:05:54,810
+الصبغة داخل البكتيريا يعني ال cooling أنا ممكن
+
+85
+00:05:54,810 --> 00:05:59,710
+أعتبره as Mordant بالإضافة لإشي تاني هنتعرف عليه
+
+86
+00:05:59,710 --> 00:06:03,650
+هيعتبر كمان ك Mordant يبقى أول إشي باعتبره ك
+
+87
+00:06:03,650 --> 00:06:07,030
+Mordant هو ال cooling ال primary stain قلنا هي
+
+88
+00:06:07,030 --> 00:06:10,590
+Carbolfoxin لكن في ال gram stain كانت crystal
+
+89
+00:06:10,590 --> 00:06:16,150
+violetفي الـ Mordant في الـ gram stain كان iodine
+
+90
+00:06:16,150 --> 00:06:20,810
+لكن في ال acid fast stain ال cooling هو as Mordant
+
+91
+00:06:20,810 --> 00:06:26,050
+بعدها بقولي decalorized with acid alcohol بضيف ال
+
+92
+00:06:26,050 --> 00:06:30,830
+decalorizer بقولي هان ما بعمل wash بال water زي ال
+
+93
+00:06:30,830 --> 00:06:35,170
+gram stain على طول بضيف ال decalorizer بعد ما تبرد
+
+94
+00:06:35,170 --> 00:06:41,310
+ال slime بحطها 15 second في acid alcoholفي الـ
+
+95
+00:06:41,310 --> 00:06:44,990
+Gram Stain كانت الـ Decalarizer ألكحول فقط اللي هو
+
+96
+00:06:44,990 --> 00:06:48,710
+Ethanol لكن في الـ Acid Fast Stain أسد ألكحول
+
+97
+00:06:48,710 --> 00:06:56,250
+الأسد 3% HCl والألكحول 95% Ethanol بعد ما مرور الـ
+
+98
+00:06:56,250 --> 00:07:01,550
+15 Second بغسل بطاب و أترق بخطوة بضيف الـ Counter
+
+99
+00:07:01,550 --> 00:07:04,330
+Stain أو الـ Secondary Stain اللي هي في الـ Acid
+
+100
+00:07:04,330 --> 00:07:10,080
+Fast Stain الـ Methylene Blue لمدة 30 Secondفي الـ
+
+101
+00:07:10,080 --> 00:07:14,500
+Gram Stain كانت عبارة عن صفرانين بغسل بعد مرور الـ
+
+102
+00:07:14,500 --> 00:07:19,220
+30 second أو الـ one minute بالـ tap water air
+
+103
+00:07:19,220 --> 00:07:23,500
+-drying بشوفها تحت الميكروسكوب على عدسة 40X وعدسة
+
+104
+00:07:23,500 --> 00:07:30,220
+100X اللي هي الـ oil immersion الـ
+
+105
+00:07:30,220 --> 00:07:34,340
+reagents used are Diel-Nelson Carbolfoxane الـ
+
+106
+00:07:34,340 --> 00:07:37,760
+Primary Stain Carbolfoxaneالـ Acid Alcohol
+
+107
+00:07:37,760 --> 00:07:42,480
+Decolorizer and Methylene Blue الـ Secondary Stain
+
+108
+00:07:42,480 --> 00:07:45,720
+أو الـ Counter Stain قولنا الـ Cooling is more
+
+109
+00:07:45,720 --> 00:07:49,860
+dense Acid Phospholipase will be bright red after
+
+110
+00:07:49,860 --> 00:07:53,280
+staining الـ Acid Phospholipase اللي هي الـ
+
+111
+00:07:53,280 --> 00:07:57,660
+Mycobacterium قولنا بتاخد اللون الأحمر بتظهر
+
+112
+00:07:57,660 --> 00:08:02,000
+محتفظة بالـ Primary Stain الكاربولفوكسين لأن قولنا
+
+113
+00:08:02,000 --> 00:08:07,900
+هي صعبة صبغتها لكن قدرنا نصبغهاوصعب إزالة اللون
+
+114
+00:08:07,900 --> 00:08:11,960
+منها لأنها مقاومة للحمد الحمد اللي هو الـ
+
+115
+00:08:11,960 --> 00:08:17,000
+Decalarizer قولنا أسد الكحول الأسد 3% HCl مقاومة
+
+116
+00:08:17,000 --> 00:08:21,600
+للحمد القوي ما راح يقدر يزيل اللون منها فهتظل
+
+117
+00:08:21,600 --> 00:08:24,980
+محتفظة باللون الأحمر بالـ Primary Stain
+
+118
+00:08:34,060 --> 00:08:37,240
+Acid fast stain are useful in identification of
+
+119
+00:08:37,240 --> 00:08:40,860
+acid fast bacilli قلنا إن الـ Acid fast stain هي
+
+120
+00:08:40,860 --> 00:08:44,660
+أداة مفيدة في تشخيص الـ Acid fast bacilli اللي هي
+
+121
+00:08:44,660 --> 00:08:48,360
+الـ Mycobacterium tuberculosis لكن لـ Gram stain
+
+122
+00:08:48,360 --> 00:08:51,180
+ما بقدر أقول إنها أداة مفيدة في ال identification
+
+123
+00:08:51,180 --> 00:08:55,660
+ما بقدر من خلالها أتعرف على هوية البكتيريا لـ Gram
+
+124
+00:08:55,660 --> 00:08:59,440
+stain هي أول خطوة من خطوات تعريف البكتيريا هي ال
+
+125
+00:08:59,440 --> 00:09:03,710
+first step in identificationلكن عشان أعمل تعريف
+
+126
+00:09:03,710 --> 00:09:07,690
+للبكتيريا لازم أسرع العين على ميديا و تنمو و بعد
+
+127
+00:09:07,690 --> 00:09:11,090
+24 ساعة أشوف نمو المستعمرات على الطبق و أشرف
+
+128
+00:09:11,090 --> 00:09:14,790
+الحصاد الكيميائية من خلالها أتعرف على البكتيريا
+
+129
+00:09:14,790 --> 00:09:18,850
+الـ Mycobacterium tuberculosis حتى تنمو بتحتاج من
+
+130
+00:09:18,850 --> 00:09:24,410
+4 ل 6 أسابيع تقريبا كل البكتيريا بتحتاج 24 ساعة
+
+131
+00:09:24,410 --> 00:09:28,070
+فقط حتى تنمو لكن الـ Mycobacterium tuberculosis
+
+132
+00:09:28,070 --> 00:09:33,280
+بتحتاج من 4 ل 6 أسابيع حتى تنمونتيجة وجود الـ wax
+
+133
+00:09:33,280 --> 00:09:37,480
+lipid الـ wax lipid بيعمل كحاجز ببطء من وصول
+
+134
+00:09:37,480 --> 00:09:42,720
+المغذيات للبكتيريا فبتاخد وقت أطول حتى تنمو عشان
+
+135
+00:09:42,720 --> 00:09:48,360
+هيك الدكتور بيطلب فحص ال acid fast stand كفحص سريع
+
+136
+00:09:48,360 --> 00:09:52,820
+رابد مش هيستنى 6 أسابيع يستنى نمو البكتيريا خلال
+
+137
+00:09:52,820 --> 00:09:57,740
+نص ساعة بتطلع نتيجة ال acid fast stand preliminary
+
+138
+00:09:57,740 --> 00:10:03,780
+diagnosis of tuberculosis هو فحصأولي لأنه لازم أنا
+
+139
+00:10:03,780 --> 00:10:07,840
+بعدين أعمل مزرعة أزرع البكتيريا و تنمو و أعمل
+
+140
+00:10:07,840 --> 00:10:11,260
+فحوصات كيميائية عشان أتأكد أنه هي Mycobacterium
+
+141
+00:10:11,260 --> 00:10:15,740
+tuberculosis و the greater than 90% productive
+
+142
+00:10:15,740 --> 00:10:19,880
+value from sputum sample بقولّي أنه لو انت عملتي
+
+143
+00:10:19,880 --> 00:10:24,780
+ال acid fastest أنه طلعت acid fast بسلعي 90% بنحكي
+
+144
+00:10:24,780 --> 00:10:28,500
+أنه هي البكتيريا Mycobacterium tuberculosis طب ليش
+
+145
+00:10:28,500 --> 00:10:32,870
+مش 100%؟لأنه لازم نزرع البكتيريا ونشوف نمو
+
+146
+00:10:32,870 --> 00:10:36,690
+البكتيريا على الطبق ونعمل فحوصات نتأكد أن هي
+
+147
+00:10:36,690 --> 00:10:41,770
+Mycobacterium tuberculosis هيك بأحكائنه نسبة 100%
+
+148
+00:10:41,770 --> 00:10:46,050
+وحتى نتيجة الـ Acid fast stand ما بنكتب النتيجة ال
+
+149
+00:10:46,050 --> 00:10:49,030
+positive لو كانت بصل لاي ألونها أحمر ما بكتب
+
+150
+00:10:49,030 --> 00:10:54,130
+Mycobacterium غلط بنكتب Acid fast بصل لاي بصل لاي
+
+151
+00:10:54,130 --> 00:10:58,640
+لأن Mycobacterium tuberculosis شكلها بصل لايالـ
+
+152
+00:10:58,640 --> 00:11:03,140
+Acid Fast معناها أنها بتقاوم الحمض الحمض اللي هو 3
+
+153
+00:11:03,140 --> 00:11:10,360
+% HCl الـ Decolorizer عشان هي احتفظت باللون لون
+
+154
+00:11:10,360 --> 00:11:13,820
+الـ Carb والفوكسين لبريمارستين وماقدر حتى الحمض
+
+155
+00:11:13,820 --> 00:11:18,420
+القوي الـ HCl يزيل هذا اللون طبعا هذه الخاصية أنها
+
+156
+00:11:18,420 --> 00:11:22,020
+بتقاوم الحمض خاصية من خصائص الـ Mycobacterium
+
+157
+00:11:22,020 --> 00:11:25,660
+tuberculosis من خلال هذه الخاصية قدرت أتعرف على
+
+158
+00:11:25,660 --> 00:11:30,930
+البكتيرياكيف انا بدي اكتب نتيجة الـacid fast stain
+
+159
+00:11:30,930 --> 00:11:34,610
+من خلال هذا الجدول هنتعرف بقولك لو فحصتي في ال
+
+160
+00:11:34,610 --> 00:11:39,690
+field وماجفتي اي acid fast بصلاي بصلاي شكلها بصلاي
+
+161
+00:11:39,690 --> 00:11:44,410
+acid fast يعني بتاخد اللون الأحمر بتكتبي نتيجة no
+
+162
+00:11:44,410 --> 00:11:48,710
+acid fast stain ممنوع تكتبيه negative لأنه ممكن
+
+163
+00:11:48,710 --> 00:11:54,290
+يكون شغلك غلط ممكن طريقة جمعك للعينة غلط تكتبي no
+
+164
+00:11:54,290 --> 00:11:58,870
+acid fast stain معناههذه الجملة إنك انت فحصت بال
+
+165
+00:11:58,870 --> 00:12:03,910
+field وما شفت أي بكتيريا لو فحصت 300 field وكانت
+
+166
+00:12:03,910 --> 00:12:09,150
+البكتيريا من 1 ل 2 acid-fast-bucyllite بتكون
+
+167
+00:12:09,150 --> 00:12:13,270
+العينة دبط full مشكوك فيها بقولك عيدي الفحص ال
+
+168
+00:12:13,270 --> 00:12:17,430
+acid-fast-stain marker تأتي لو فحصت 100 field وكان
+
+169
+00:12:17,430 --> 00:12:22,740
+من 1 ل 9 acid-fast-bucyllite تعطي plus oneلو عشر
+
+170
+00:12:22,740 --> 00:12:25,840
+في الـ data حصلت وكان acid fast بصلاي من واحد
+
+171
+00:12:25,840 --> 00:12:29,620
+لتسعة plus two لو feed واحد كان فيه من واحد لتسعة
+
+172
+00:12:29,620 --> 00:12:33,120
+acid fast بصلاي plus three لو ال feed الواحد كان
+
+173
+00:12:33,120 --> 00:12:39,180
+أكتر من تسعة بتكون plus four بقولنا it also can be
+
+174
+00:12:39,180 --> 00:12:42,540
+performed in patients able to track the progress
+
+175
+00:12:42,540 --> 00:12:46,440
+of antibiotic therapy بقولنا أن ال acid fast 10
+
+176
+00:12:46,440 --> 00:12:50,840
+ممكن من خلالها أشوف استجابة المريض للعلاج على سبيل
+
+177
+00:12:50,840 --> 00:12:55,760
+المثالالمريض كان plus three والدكتور وصف له علاج
+
+178
+00:12:55,760 --> 00:13:01,340
+بعد مدة معينة طلب الفحص مرة تانية لازم حتى أن
+
+179
+00:13:01,340 --> 00:13:05,960
+المريض يكون استجاب للعلاج لازم يكون plus two أو
+
+180
+00:13:05,960 --> 00:13:10,440
+plus one and determine their degree of
+
+181
+00:13:10,440 --> 00:13:14,430
+contagiousnessماذا يعني كونتاجستنس؟ كونتاجستنس
+
+182
+00:13:14,430 --> 00:13:19,410
+معناها شراسة المرض أكيد الـPlus-4 هتكون أكتر شراسة
+
+183
+00:13:19,410 --> 00:13:23,430
+من الـPlus-2 يبقى من خلال الـGram أو من خلال
+
+184
+00:13:23,430 --> 00:13:28,130
+الـAcid-fast-strain بقدر أتعرف على قوة شراسة المرض
+
+185
+00:13:28,130 --> 00:13:34,630
+Acid
+
+186
+00:13:34,630 --> 00:13:39,840
+-fast-reagent أول reagent للـCarbوفكسينتعطي اللون
+
+187
+00:13:39,840 --> 00:13:43,720
+الـ Red a phenolic stain لأنه أحد مكونات الصبغة
+
+188
+00:13:43,720 --> 00:13:47,380
+الـ phenol is the primary stain in the acid fast
+
+189
+00:13:47,380 --> 00:13:51,280
+test هو الصبغة الأولية اللي بنستخدمها في ال acid
+
+190
+00:13:51,280 --> 00:13:55,340
+fast test it is soluble in the lipid of the
+
+191
+00:13:55,340 --> 00:13:59,580
+mycobacterial cell wall بقولنا هذه الصبغة بتدوب في
+
+192
+00:13:59,580 --> 00:14:02,900
+الدهون الموجودة على ال cell wall تبع ال
+
+193
+00:14:02,900 --> 00:14:07,020
+mycobacterium tuberculosis يبقى هي lipid soluble
+
+194
+00:14:07,310 --> 00:14:11,230
+هذا هيساعد في اختراق الصبغة الـ Carbol Fuxin
+
+195
+00:14:11,230 --> 00:14:15,610
+للواكس لبن it is consists of basic Fuxin بقولك
+
+196
+00:14:15,610 --> 00:14:19,450
+مكونات هذه الصبغة basic Fuxin هو اللي بيعطي اللون
+
+197
+00:14:19,450 --> 00:14:24,450
+on carbolic acid الفينول الفينول يعتبر as mordant
+
+198
+00:14:24,450 --> 00:14:29,090
+مع ال cooling هيثبت ال Carbol Fuxin داخل البكتيريا
+
+199
+00:14:29,090 --> 00:14:33,140
+heating the specimenبقولنا بتم تسخين العينة
+
+200
+00:14:33,140 --> 00:14:35,860
+وتعريضها للـ steam عشان يزيد من اختراق
+
+201
+00:14:35,860 --> 00:14:39,240
+الكربوفوكسين للـ wax lipid طب لو أنا ما بنتي
+
+202
+00:14:39,240 --> 00:14:42,640
+أعرضها للـ steam بقولك ممكن أضيفي wetting agent
+
+203
+00:14:42,640 --> 00:14:47,760
+such as tergatol ممكن أنضيف tergatol هيعمل نفس عمل
+
+204
+00:14:47,760 --> 00:14:50,640
+لـ steam increase the penetration of the
+
+205
+00:14:50,640 --> 00:14:54,460
+kerbofoxin هيزيد من اختراق الصبقة للـ wax lipid
+
+206
+00:14:54,460 --> 00:14:58,040
+بجيب slide و بحط drop of tergatol و ما بحطها على
+
+207
+00:14:58,040 --> 00:15:07,590
+إلاهابعد مضيف الكربوفوكسين العينة منبردها وهذا
+
+208
+00:15:07,590 --> 00:15:11,690
+بيكون as mordant بعدها بدي أضيف الـ decolorizer
+
+209
+00:15:11,690 --> 00:15:17,490
+اللي هو أسد ألكحول مش ألكحول لحال الأسد 3% HCl
+
+210
+00:15:17,490 --> 00:15:21,030
+والألكحول 95% Ethanol
+
+211
+00:15:25,760 --> 00:15:28,880
+بالرغم من أن الكربوفوكسين أكثر مستعملable في الوكس
+
+212
+00:15:28,880 --> 00:15:33,620
+الليبد من في الاسد الكحول ذوبانية الكربوفوكسين في
+
+213
+00:15:33,620 --> 00:15:37,280
+الوكس الليبد أعلى من ذوبانيتها في الاسد الكحول
+
+214
+00:15:37,280 --> 00:15:41,760
+الاسد الكحول يزيل الكربوفوكسين من نوع من الأجسام
+
+215
+00:15:41,760 --> 00:15:42,340
+الليبدية من نوع من الأجسام الليبدية من نوع من
+
+216
+00:15:42,340 --> 00:15:45,860
+الأجسام الليبدية من نوع من الأجسام الليبدية من نوع
+
+217
+00:15:45,860 --> 00:15:54,980
+من الأجسام الليبدية من نوع من الأجسام الليبديةلكن
+
+218
+00:15:54,980 --> 00:15:58,080
+ماقدر يزيل اللون من الـ Acid Phospholipid-Bacillus
+
+219
+00:15:58,080 --> 00:16:00,260
+الـ Acid Phospholipid-Bacillus زي الـ
+
+220
+00:16:00,260 --> 00:16:03,580
+Mycobacterium tuberculosis بتحتوي على واكسي لبت
+
+221
+00:16:03,580 --> 00:16:07,880
+الكاربولفوكسين هتدوب في هذه الواكسي لبت بعدها هضيف
+
+222
+00:16:07,880 --> 00:16:11,940
+الـ Decolorizer اللي هو الاسد الكحول فمش هيقدر
+
+223
+00:16:11,940 --> 00:16:15,640
+يزيل اللون منها اللون صبغة الكاربولفوكسين لأن
+
+224
+00:16:15,640 --> 00:16:20,740
+الكاربولفوكسين زابت بالاصل في الواكسي لبتفمش هقدر
+
+225
+00:16:20,740 --> 00:16:24,040
+أن أفصل الـ Carbolfoxin عن الـ Wax Lipid زي السكر
+
+226
+00:16:24,040 --> 00:16:27,160
+لما يدوب في الميه مابقدر أفصلهم عن بعض فلما يجي ال
+
+227
+00:16:27,160 --> 00:16:31,740
+Decalarizer بعدها هيكون ال Carbofoxin دائم في ال
+
+228
+00:16:31,740 --> 00:16:36,100
+Wax Lipid مش هيقدر يزيل اللون لكن الـ Non-acid
+
+229
+00:16:36,100 --> 00:16:40,540
+fast organism ما بتحتوي على Wax Lipid فأول إشي
+
+230
+00:16:40,540 --> 00:16:44,180
+هصبغها ب Primers تان ل Carbofoxin هتاخد الصبغة بكل
+
+231
+00:16:44,180 --> 00:16:47,960
+سهولة بعدها هنضيف ال Decalarizer ال Acid Alcohol
+
+232
+00:16:48,430 --> 00:16:52,190
+الـ Carbolfoxin هيدوب في الـ acid alcohol لأنه أنا
+
+233
+00:16:52,190 --> 00:16:55,890
+ماعندي wax lipid فاللون هيروح بكل سهولة و هتصير
+
+234
+00:16:55,890 --> 00:16:59,850
+شفّع فهضطر أصبغها بـ counter stain with methylene
+
+235
+00:16:59,850 --> 00:17:02,650
+blue الـ methylene blue هي ال counter stain which
+
+236
+00:17:02,650 --> 00:17:05,850
+cannot penetrate mycolic acid مابتقدر تخترق ال
+
+237
+00:17:05,850 --> 00:17:08,730
+mycolic acid ال waxy lipid لأنه هي مش lipid
+
+238
+00:17:08,730 --> 00:17:13,030
+soluble زي ال Carbolfoxin وماعرضت الصبغة لبخار
+
+239
+00:17:13,030 --> 00:17:17,010
+provide contrast to non acid fast cell بقولك هذا
+
+240
+00:17:18,070 --> 00:17:23,790
+بميزها و هي بتعطي اللون لـ non acid fast cell و
+
+241
+00:17:23,790 --> 00:17:27,390
+بقدر انا افرقها بين ال acid fast بس ال lie و non
+
+242
+00:17:27,390 --> 00:17:31,850
+acid fast بس ال lie Mycobacteria are difficult to
+
+243
+00:17:31,850 --> 00:17:35,970
+stain ال mycobacteria صعب صباغتها but once stained
+
+244
+00:17:35,970 --> 00:17:43,000
+لو انا قدرت أصبغها زي ما قدرنا في المعملصعب إزالة
+
+245
+00:17:43,000 --> 00:17:47,820
+اللون منها حتى في وجود
+
+246
+00:17:47,820 --> 00:17:53,460
+حمض قوي صعب إن أحنا نزيل اللون من الميكوبكتيريوم
+
+247
+00:17:53,460 --> 00:17:58,120
+وهي طبعا خاصية أو ميزة من خلالها قدرت أتعرف على
+
+248
+00:17:58,120 --> 00:18:03,000
+البكتيريوم بالنسبة
+
+249
+00:18:03,000 --> 00:18:06,180
+للخطوات Smelly preparation, air-drying, heat
+
+250
+00:18:06,180 --> 00:18:11,240
+-fixation البراميرستان الكربوفكسينحتى انه تساعد
+
+251
+00:18:11,240 --> 00:18:15,680
+اختراق الكربوفوكسين للوقس اللي بضيف تيرجيتول لمدة
+
+252
+00:18:15,680 --> 00:18:20,560
+خمس دقائق لو ماعندي تيرجيتول بغرد ال slide ل ال
+
+253
+00:18:20,560 --> 00:18:26,120
+steam لمدة تمان دقائق بعدها بعمل cooling تبريد ل
+
+254
+00:18:26,120 --> 00:18:31,380
+ال slide بكون as more than اكسد الكحول اللي هو ال
+
+255
+00:18:31,380 --> 00:18:35,340
+decolorizer لمدة خمس عشر لعشرين second بعدها بغسل
+
+256
+00:18:35,340 --> 00:18:39,650
+بال water بضيف ال counterاللي نقبله لمدة one
+
+257
+00:18:39,650 --> 00:18:43,250
+minute او تلاتين second بغسل بال tap water air
+
+258
+00:18:43,250 --> 00:18:46,050
+drying و بشوفها تحت ال microscope على ال oil
+
+259
+00:18:46,050 --> 00:18:49,910
+immersion positive result a pair pink or red بتكون
+
+260
+00:18:49,910 --> 00:18:54,190
+لازم تكون bustle line negative organism بتظهر كلون
+
+261
+00:18:54,190 --> 00:18:56,590
+أزرق كوكاي أو bustle line
+
+262
+00:19:00,870 --> 00:19:05,210
+قبل ال-staining البكتيريا بتكون transparent صبغت
+
+263
+00:19:05,210 --> 00:19:09,310
+بي الكاربولفوكسين بالـprimary stain سواء كانت acid
+
+264
+00:19:09,310 --> 00:19:13,490
+-fast-bacilli أو non-acid-fast-bacilli هتاخد اللون
+
+265
+00:19:13,490 --> 00:19:16,790
+الـred بعدها ضفت الـdecolorizer اللي هو الاسد
+
+266
+00:19:16,790 --> 00:19:21,430
+الكحول هينزل اللون من الـnon-acid-fast-bacilli لأن
+
+267
+00:19:21,430 --> 00:19:25,940
+سرعة ذوبانية الكاربولفوكسينفي الـ wax lipid أعلى
+
+268
+00:19:25,940 --> 00:19:29,540
+من الاسد الكحول بالنسبة لـ Non-acid fast-bust lima
+
+269
+00:19:29,540 --> 00:19:33,300
+بتحتوي على wax lipid فبالتالي الكربوفوكسين هيدوب
+
+270
+00:19:33,300 --> 00:19:37,600
+في الاسد الكحول و اللون هيروح لكن الاسد fast-bust
+
+271
+00:19:37,600 --> 00:19:42,080
+lime البكتيريا بتحتوي على wax lipid الكربوفوكسين
+
+272
+00:19:42,080 --> 00:19:45,660
+هيدوب في ال wax lipid هييجي ال decolorizer مش
+
+273
+00:19:45,660 --> 00:19:49,260
+هيقدر يزيل اللون بعدها هضيف ال counterstain
+
+274
+00:19:49,260 --> 00:19:54,040
+الميثيلين blue أكيد البكتيريا الليكانت transparent
+
+275
+00:19:54,040 --> 00:19:57,640
+شفافة فهتاخد اللون اللي هو اللون الأزرق لكن
+
+276
+00:19:57,640 --> 00:20:01,280
+البكتيريا ال acid fast basalite هتظلها محتفظة
+
+277
+00:20:01,280 --> 00:20:05,780
+باللون ال red لأنه احنا قولنا انه هي صعب ازالة
+
+278
+00:20:05,780 --> 00:20:10,500
+اللون منها فبالتالي هتظلها red و basalite لكن ال
+
+279
+00:20:10,500 --> 00:20:16,200
+non acid fast organism ممكن تكون كوكاي او basalite
+
+280
+00:20:16,200 --> 00:20:18,460
+لكن اللون، اللون blue
+
+281
+00:20:22,120 --> 00:20:26,440
+هي نتائج acid fast stain منلاحظ كل الصور هي acid
+
+282
+00:20:26,440 --> 00:20:31,220
+fast بصلاي هيك بكتب النتيجة acid fast بصلاي بصلاي
+
+283
+00:20:31,220 --> 00:20:36,040
+الشعب تبعها بصلاي عصوي و acid fast يعني قاومة
+
+284
+00:20:36,040 --> 00:20:41,900
+الحمض 3% FCL decolorizer احتفظت بلون ال primer
+
+285
+00:20:41,900 --> 00:20:46,720
+stain الكربوفوكسينومائد حتى الحمض القوي يزيل اللون
+
+286
+00:20:46,720 --> 00:20:47,180
+منها
+
+287
+00:20:51,330 --> 00:20:54,970
+Cannon Stain or cold method method of staining
+
+288
+00:20:54,970 --> 00:20:58,970
+acid fast microorganism هي طريقة لصباغة ال acid
+
+289
+00:20:58,970 --> 00:21:03,350
+fast بسلعي خاصة الميكوبكتيريا procedure is similar
+
+290
+00:21:03,350 --> 00:21:07,470
+to zel nelson stain الخطوات هي نفس خطوات ال zel
+
+291
+00:21:07,470 --> 00:21:10,850
+nelson stain اللي هي ال acid fast stain but
+
+292
+00:21:10,850 --> 00:21:14,610
+doesn't involve heating the slide لكن أنا ما بعرض
+
+293
+00:21:14,610 --> 00:21:17,770
+ال slide ل steam عشان هيك سميتها بال cold method
+
+294
+00:21:18,400 --> 00:21:22,440
+Kynon Staining method used carboxyne as a primary
+
+295
+00:21:22,440 --> 00:21:26,000
+stain استخدم الكربوفوكسين ك primary stain أسود
+
+296
+00:21:26,000 --> 00:21:29,820
+ألكوهول ك decolorizer والميتاليني بلو ك counter
+
+297
+00:21:29,820 --> 00:21:34,080
+stain طيب شو الفرق؟ الكنون كربوفوكسين has a
+
+298
+00:21:34,080 --> 00:21:37,340
+greater concentration of phenol and basicfoxine
+
+299
+00:21:37,340 --> 00:21:41,760
+بقولنا إنه فيه اندي تركيز عالي من الفينول ومن ال
+
+300
+00:21:41,760 --> 00:21:45,760
+basicfoxine اللي هم أحد مكونات الكربوفوكسين
+
+301
+00:21:45,760 --> 00:21:50,980
+فبالتاليما بحتاج الـ heat الحرارة حتى إنها تخترق
+
+302
+00:21:50,980 --> 00:21:55,760
+الـ waxy label تحت المايكروسكوب بشوف الخلايا الـ
+
+303
+00:21:55,760 --> 00:21:58,680
+acid-fast organism بتظهر باللون الأحمر و الـ non
+
+304
+00:21:58,680 --> 00:22:03,340
+-acid-fast organism بتظهر باللون الأزرق نفس نتائج
+
+305
+00:22:03,340 --> 00:22:09,940
+الـ acid-fastستانورامينرودامينستان
+
+306
+00:22:10,310 --> 00:22:13,670
+Oramine rhodamine fluorochrome staining هي الطريقة
+
+307
+00:22:13,670 --> 00:22:17,130
+التالتة حتى أني أنا أقدر أشوف ال acid fast-bacilli
+
+308
+00:22:17,130 --> 00:22:20,650
+اللي هي ال mycobacterium الطريقة الأولى كانت ال
+
+309
+00:22:20,650 --> 00:22:23,470
+acid fast-stain أو ال Zellnelson stain الطريقة
+
+310
+00:22:23,470 --> 00:22:26,290
+التانية كانت ال quinone stain و الطريقة التالتة
+
+311
+00:22:26,290 --> 00:22:30,030
+اللي هي هاي ال oramine rhodamine stain من خلال هاي
+
+312
+00:22:30,030 --> 00:22:34,090
+الطريقة بقدر أشوف ال acid fast-bacilli باستخدام ال
+
+313
+00:22:34,090 --> 00:22:37,650
+fluorescent microscope هتظهر ال mycobacterium بلون
+
+314
+00:22:37,650 --> 00:22:39,150
+أصفر reddish yellow
+
+315
+00:22:42,740 --> 00:22:46,980
+أخر معلومة في الـ Acid Fast StainThe reddish pink
+
+316
+00:22:46,980 --> 00:22:50,720
+color and carding sticking together in long ruby
+
+317
+00:22:50,720 --> 00:22:54,320
+masses of the mycobacterium cell due to the excess
+
+318
+00:22:54,320 --> 00:22:57,240
+lipid of the cell wall بقولنا في انديانا طبقة
+
+319
+00:22:57,240 --> 00:23:01,280
+شمعية بتخلي البكتيريا تلزق بعض بتكون مبينة على شكل
+
+320
+00:23:01,280 --> 00:23:05,780
+الحبل carding تمام هذه الظاهرة اسمها الـ ruby
+
+321
+00:23:05,780 --> 00:23:10,730
+masses أو ممكن نسميها cardingهذه الظاهرة تكون في
+
+322
+00:23:10,730 --> 00:23:14,790
+الـ Mycobacterium نتيجة الـ high concentration of
+
+323
+00:23:14,790 --> 00:23:15,410
+lipid
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/4-SUdsxRvPI_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/4-SUdsxRvPI_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..cf72b040dab54bb370187c0973c713fdc6000c70
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/4-SUdsxRvPI_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 2820, "start": 1.48, "end": 28.2, "text": "Acid Fast Stain أو الإسم الآخر Zell-Nelson Stain علمان اكتشفوا هذه الصبغة فصميت باسمها The Acid Fast Stain is another differential staining method اتفقنا إنه هي نوع من أنواع الـDifferential Stain اللي هتقسم البكتيريا لمجموعة فهتقسم البكتيريا ل Acid Fast بس اللهي وNon Acid Fast بس اللهي في الإجرام الستان قلنا إنه مش كل البكتيريا بقدر أصبغها", "tokens": [43705, 327, 15968, 745, 491, 34051, 33688, 38251, 6024, 95, 34740, 1176, 898, 12, 45, 20471, 745, 491, 11203, 2304, 7649, 1975, 4117, 2655, 8592, 5172, 14407, 29538, 31767, 3555, 17082, 3660, 6156, 9381, 2304, 36081, 4724, 32277, 2304, 11296, 440, 5097, 327, 15968, 745, 491, 307, 1071, 15756, 342, 3686, 3170, 1975, 2655, 5172, 4587, 8315, 36145, 3224, 39896, 8717, 45367, 9154, 14739, 14407, 3615, 2423, 39184, 35, 15790, 831, 745, 491, 13672, 1829, 8032, 2655, 4587, 38251, 29739, 4117, 2655, 13546, 25528, 32767, 7435, 2304, 45367, 3660, 6156, 3224, 2655, 4587, 38251, 29739, 4117, 2655, 13546, 25528, 5296, 5097, 327, 15968, 4724, 3794, 21984, 1829, 4032, 47914, 5097, 327, 15968, 4724, 3794, 21984, 1829, 8978, 33688, 7435, 2288, 10943, 2423, 14851, 7649, 12174, 1211, 8315, 36145, 3224, 37893, 28242, 29739, 4117, 2655, 13546, 25528, 4724, 28543, 2288, 5551, 9381, 3555, 17082, 11296], "avg_logprob": -0.2202586239781873, "compression_ratio": 1.795774647887324, "no_speech_prob": 2.1457672119140625e-06, "words": [{"start": 1.48, "end": 1.92, "word": "Acid", "probability": 0.6834716796875}, {"start": 1.92, "end": 2.38, "word": " Fast", "probability": 0.473388671875}, {"start": 2.38, "end": 2.9, "word": " Stain", "probability": 0.94091796875}, {"start": 2.9, "end": 3.16, "word": " أو", "probability": 0.810546875}, {"start": 3.16, "end": 3.5, "word": " الإسم", "probability": 0.7125244140625}, {"start": 3.5, "end": 4.1, "word": " الآخر", "probability": 0.9524739583333334}, {"start": 4.1, "end": 4.62, "word": " Zell", "probability": 0.560791015625}, 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أعتبره as Mordant بالإضافة لإشي تاني هنتعرف عليه هيعتبر كمان ك Mordant يبقى أول إشي باعتبره ك Mordant هو ال cooling ال primary stain قلنا هي Carbolfoxin لكن في ال gram stain كانت crystal violet", "tokens": [6027, 2655, 3555, 16572, 3215, 39896, 25957, 1211, 12883, 267, 8167, 11933, 3615, 18513, 3660, 12602, 16758, 9566, 34892, 14407, 3555, 9566, 33251, 25513, 11536, 10632, 13672, 1829, 1975, 2655, 4117, 3794, 2288, 2655, 20666, 2655, 6027, 1829, 8032, 2655, 1863, 5016, 7435, 11622, 31767, 3555, 17082, 3660, 11778, 47283, 1211, 29739, 4117, 2655, 13546, 25528, 37495, 22653, 2423, 14785, 41850, 3714, 43020, 5551, 34268, 26890, 3224, 382, 376, 765, 394, 20666, 28814, 11242, 31845, 3660, 5296, 28814, 8592, 1829, 6055, 7649, 1829, 8032, 29399, 3615, 28480, 47356, 39896, 34268, 26890, 9122, 2304, 7649, 9122, 376, 765, 394, 7251, 3555, 4587, 7578, 5551, 12610, 11933, 8592, 1829, 4724, 995, 34268, 26890, 3224, 9122, 376, 765, 394, 31439, 2423, 14785, 2423, 6194, 16441, 12174, 1211, 8315, 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355.75, "end": 356.47, "word": " Mordant", "probability": 0.7960611979166666}, {"start": 356.47, "end": 357.49, "word": " بالإضافة", "probability": 0.9712890625}, {"start": 357.49, "end": 358.51, "word": " لإشي", "probability": 0.8057861328125}, {"start": 358.51, "end": 358.77, "word": " تاني", "probability": 0.9710286458333334}, {"start": 358.77, "end": 359.35, "word": " هنتعرف", "probability": 0.9014892578125}, {"start": 359.35, "end": 359.71, "word": " عليه", "probability": 0.99267578125}, {"start": 359.71, "end": 360.31, "word": " هيعتبر", "probability": 0.8948567708333334}, {"start": 360.31, "end": 360.75, "word": " كمان", "probability": 0.97705078125}, {"start": 360.75, "end": 360.95, "word": " ك", "probability": 0.94482421875}, {"start": 360.95, "end": 361.33, "word": " Mordant", "probability": 0.7880859375}, {"start": 361.33, "end": 361.71, "word": " يبقى", "probability": 0.975341796875}, {"start": 361.71, "end": 362.23, "word": " أول", "probability": 0.959228515625}, {"start": 362.23, "end": 363.01, "word": " إشي", "probability": 0.9361979166666666}, {"start": 363.01, "end": 363.51, "word": " باعتبره", "probability": 0.880712890625}, {"start": 363.51, "end": 363.65, "word": " ك", "probability": 0.95849609375}, {"start": 363.65, "end": 364.07, "word": " Mordant", "probability": 0.8857421875}, {"start": 364.07, "end": 364.33, "word": " هو", "probability": 0.98095703125}, {"start": 364.33, "end": 364.65, "word": " ال", "probability": 0.94482421875}, {"start": 364.65, "end": 365.11, "word": " cooling", "probability": 0.9755859375}, {"start": 365.11, "end": 365.65, "word": " ال", "probability": 0.8564453125}, {"start": 365.65, "end": 366.01, "word": " primary", "probability": 0.359375}, {"start": 366.01, "end": 366.41, "word": " stain", "probability": 0.7314453125}, {"start": 366.41, "end": 366.79, "word": " قلنا", "probability": 0.9129231770833334}, {"start": 366.79, "end": 367.03, "word": " هي", "probability": 0.921875}, {"start": 367.03, "end": 368.17, "word": " Carbolfoxin", "probability": 0.6189453125}, {"start": 368.17, "end": 368.79, "word": " لكن", "probability": 0.91259765625}, {"start": 368.79, "end": 369.05, "word": " في", "probability": 0.95263671875}, {"start": 369.05, "end": 369.15, "word": " ال", "probability": 0.7451171875}, {"start": 369.15, "end": 369.41, "word": " gram", "probability": 0.167724609375}, {"start": 369.41, "end": 369.73, "word": " stain", "probability": 0.8740234375}, {"start": 369.73, "end": 370.21, "word": " كانت", "probability": 0.89697265625}, {"start": 370.21, "end": 370.59, "word": " crystal", "probability": 0.36767578125}, {"start": 370.59, "end": 371.05, "word": " violet", "probability": 0.92919921875}], "temperature": 1.0}, {"id": 14, "seek": 40059, "start": 371.65, "end": 400.59, "text": "في الـ Mordant في الـ gram stain كان iodine لكن في ال acid fast stain ال cooling هو as Mordant بعدها بقولي decalorized with acid alcohol بضيف ال decalorizer بقولي هان ما بعمل wash بال water زي ال gram stain على طول بضيف ال decalorizer بعد ما تبرد ال slime بحطها 15 second في acid alcohol", "tokens": [41185, 2423, 39184, 376, 765, 394, 8978, 2423, 39184, 21353, 16441, 25961, 44422, 533, 44381, 8978, 2423, 8258, 2370, 16441, 2423, 14785, 31439, 382, 376, 765, 394, 39182, 11296, 4724, 39648, 1829, 979, 304, 284, 1602, 365, 8258, 7658, 4724, 11242, 33911, 2423, 979, 304, 284, 6545, 4724, 39648, 1829, 8032, 7649, 19446, 4724, 25957, 1211, 5675, 20666, 1281, 30767, 1829, 2423, 21353, 16441, 15844, 23032, 12610, 4724, 11242, 33911, 2423, 979, 304, 284, 6545, 39182, 19446, 6055, 26890, 3215, 2423, 20650, 4724, 5016, 9566, 11296, 2119, 1150, 8978, 8258, 7658], "avg_logprob": -0.24507472194407298, "compression_ratio": 1.766355140186916, "no_speech_prob": 6.258487701416016e-05, "words": [{"start": 371.65, "end": 371.97, "word": "في", "probability": 0.640625}, {"start": 371.97, "end": 372.89, "word": " الـ", "probability": 0.618896484375}, {"start": 372.89, "end": 374.05, "word": " Mordant", "probability": 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"word": " ال", "probability": 0.9677734375}, {"start": 386.05, "end": 387.07, "word": " decalorizer", "probability": 0.8671875}, {"start": 387.07, "end": 387.87, "word": " بقولي", "probability": 0.9215494791666666}, {"start": 387.87, "end": 388.19, "word": " هان", "probability": 0.7529296875}, {"start": 388.19, "end": 388.39, "word": " ما", "probability": 0.56640625}, {"start": 388.39, "end": 388.95, "word": " بعمل", "probability": 0.9348958333333334}, {"start": 388.95, "end": 389.35, "word": " wash", "probability": 0.904296875}, {"start": 389.35, "end": 389.73, "word": " بال", "probability": 0.615234375}, {"start": 389.73, "end": 390.15, "word": " water", "probability": 0.264892578125}, {"start": 390.15, "end": 390.73, "word": " زي", "probability": 0.8505859375}, {"start": 390.73, "end": 390.83, "word": " ال", "probability": 0.86865234375}, {"start": 390.83, "end": 391.11, "word": " gram", "probability": 0.80322265625}, {"start": 391.11, "end": 391.55, "word": " stain", "probability": 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0.3680419921875}, {"start": 425.29, "end": 425.79, "word": " Methylene", "probability": 0.729736328125}, {"start": 425.79, "end": 426.25, "word": " Blue", "probability": 0.92724609375}, {"start": 426.25, "end": 427.15, "word": " لمدة", "probability": 0.939697265625}, {"start": 427.15, "end": 427.83, "word": " 30", "probability": 0.94775390625}, {"start": 427.83, "end": 428.55, "word": " Second", "probability": 0.8896484375}], "temperature": 1.0}, {"id": 16, "seek": 45638, "start": 429.32, "end": 456.38, "text": "في الـ Gram Stain كانت عبارة عن صفرانين بغسل بعد مرور الـ 30 second أو الـ one minute بالـ tap water air-drying بشوفها تحت الميكروسكوب على عدسة 40X وعدسة 100X اللي هي الـ oil immersion الـ reagents used are Diel-Nelson Carbolfoxane الـ Primary Stain Carbolfoxane", "tokens": [41185, 2423, 39184, 22130, 745, 491, 25961, 2655, 6225, 3555, 9640, 3660, 18871, 20328, 5172, 2288, 7649, 9957, 4724, 17082, 3794, 1211, 39182, 3714, 2288, 13063, 2423, 39184, 2217, 1150, 34051, 2423, 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444.68, "word": " 100X", "probability": 0.905029296875}, {"start": 444.68, "end": 444.82, "word": " اللي", "probability": 0.93310546875}, {"start": 444.82, "end": 445.06, "word": " هي", "probability": 0.9248046875}, {"start": 445.06, "end": 445.3, "word": " الـ", "probability": 0.687255859375}, {"start": 445.3, "end": 445.66, "word": " oil", "probability": 0.78515625}, {"start": 445.66, "end": 446.36, "word": " immersion", "probability": 0.43212890625}, {"start": 446.36, "end": 450.22, "word": " الـ", "probability": 0.5166015625}, {"start": 450.22, "end": 450.82, "word": " reagents", "probability": 0.36798095703125}, {"start": 450.82, "end": 451.32, "word": " used", "probability": 0.92333984375}, {"start": 451.32, "end": 451.84, "word": " are", "probability": 0.93701171875}, {"start": 451.84, "end": 452.4, "word": " Diel", "probability": 0.283203125}, {"start": 452.4, "end": 452.86, "word": "-Nelson", "probability": 0.5398763020833334}, {"start": 452.86, "end": 454.04, "word": " Carbolfoxane", "probability": 0.4632080078125}, {"start": 454.04, "end": 454.34, "word": " الـ", "probability": 0.71044921875}, {"start": 454.34, "end": 454.74, "word": " Primary", "probability": 0.321533203125}, {"start": 454.74, "end": 455.26, "word": " Stain", "probability": 0.797607421875}, {"start": 455.26, "end": 456.38, "word": " Carbolfoxane", "probability": 0.83134765625}], "temperature": 1.0}, {"id": 17, "seek": 48558, "start": 456.7, "end": 485.58, "text": "الـ Acid Alcohol Decolorizer and Methylene Blue الـ Secondary Stain أو الـ Counter Stain قولنا الـ Cooling is more dense Acid Phospholipase will be bright red after staining الـ Acid Phospholipase اللي هي الـ Mycobacterium قولنا بتاخد اللون الأحمر بتظهر محتفظة بالـ Primary Stain الكاربولفوكسين لأن قولنا هي صعبة صبغتها لكن قدرنا نصبغها", "tokens": [6027, 39184, 5097, 327, 48779, 12427, 36182, 6545, 293, 48602, 46830, 8510, 2423, 39184, 5736, 822, 745, 491, 34051, 2423, 39184, 35607, 745, 491, 12174, 12610, 8315, 2423, 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459.16, "word": " Decolorizer", "probability": 0.6399739583333334}, {"start": 459.16, "end": 459.5, "word": " and", "probability": 0.47119140625}, {"start": 459.5, "end": 460.02, "word": " Methylene", "probability": 0.48712158203125}, {"start": 460.02, "end": 460.5, "word": " Blue", "probability": 0.85693359375}, {"start": 460.5, "end": 461.3, "word": " الـ", "probability": 0.680908203125}, {"start": 461.3, "end": 461.9, "word": " Secondary", "probability": 0.685791015625}, {"start": 461.9, "end": 462.48, "word": " Stain", "probability": 0.877197265625}, {"start": 462.48, "end": 462.8, "word": " أو", "probability": 0.873046875}, {"start": 462.8, "end": 463.1, "word": " الـ", "probability": 0.919677734375}, {"start": 463.1, "end": 463.5, "word": " Counter", "probability": 0.7529296875}, {"start": 463.5, "end": 464.06, "word": " Stain", "probability": 0.95166015625}, {"start": 464.06, "end": 464.4, "word": " قولنا", "probability": 0.8577473958333334}, {"start": 464.4, "end": 464.54, 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"probability": 0.76104736328125}, {"start": 477.66, "end": 478.56, "word": " محتفظة", "probability": 0.87587890625}, {"start": 478.56, "end": 478.96, "word": " بالـ", "probability": 0.6038818359375}, {"start": 478.96, "end": 479.44, "word": " Primary", "probability": 0.92333984375}, {"start": 479.44, "end": 480.06, "word": " Stain", "probability": 0.9365234375}, {"start": 480.06, "end": 481.14, "word": " الكاربولفوكسين", "probability": 0.6936577690972222}, {"start": 481.14, "end": 481.66, "word": " لأن", "probability": 0.861328125}, {"start": 481.66, "end": 482.0, "word": " قولنا", "probability": 0.93994140625}, {"start": 482.0, "end": 482.22, "word": " هي", "probability": 0.85400390625}, {"start": 482.22, "end": 482.92, "word": " صعبة", "probability": 0.9046223958333334}, {"start": 482.92, "end": 483.6, "word": " صبغتها", "probability": 0.8875}, {"start": 483.6, "end": 483.9, "word": " لكن", "probability": 0.90087890625}, {"start": 483.9, "end": 484.56, "word": " قدرنا", 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745, 491], "avg_logprob": -0.20296224392950535, "compression_ratio": 1.6839378238341969, "no_speech_prob": 4.76837158203125e-07, "words": [{"start": 486.08, "end": 486.92, "word": "وصعب", "probability": 0.8316650390625}, {"start": 486.92, "end": 487.44, "word": " إزالة", "probability": 0.9207763671875}, {"start": 487.44, "end": 487.9, "word": " اللون", "probability": 0.9599609375}, {"start": 487.9, "end": 488.54, "word": " منها", "probability": 0.974853515625}, {"start": 488.54, "end": 489.4, "word": " لأنها", "probability": 0.8924153645833334}, {"start": 489.4, "end": 490.38, "word": " مقاومة", "probability": 0.94755859375}, {"start": 490.38, "end": 490.94, "word": " للحمد", "probability": 0.7906901041666666}, {"start": 490.94, "end": 491.48, "word": " الحمد", "probability": 0.8841145833333334}, {"start": 491.48, "end": 491.58, "word": " اللي", "probability": 0.6478271484375}, {"start": 491.58, "end": 491.8, "word": " هو", "probability": 0.94677734375}, {"start": 491.8, "end": 491.96, "word": " الـ", "probability": 0.71435546875}, {"start": 491.96, "end": 492.8, "word": " Decalarizer", "probability": 0.56500244140625}, {"start": 492.8, "end": 493.22, "word": " قولنا", "probability": 0.7674153645833334}, {"start": 493.22, "end": 493.72, "word": " أسد", "probability": 0.6878255208333334}, {"start": 493.72, "end": 494.22, "word": " الكحول", "probability": 0.84423828125}, {"start": 494.22, "end": 494.64, "word": " الأسد", "probability": 0.7347005208333334}, {"start": 494.64, "end": 495.04, "word": " 3", "probability": 0.81591796875}, {"start": 495.04, "end": 495.5, "word": "%", "probability": 0.96533203125}, {"start": 495.5, "end": 496.22, "word": " HCl", "probability": 0.714599609375}, {"start": 496.22, "end": 497.0, "word": " مقاومة", "probability": 0.9822265625}, {"start": 497.0, "end": 497.58, "word": " للحمد", "probability": 0.9571940104166666}, {"start": 497.58, "end": 498.06, "word": " القوي", "probability": 0.888671875}, {"start": 498.06, "end": 498.56, "word": 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{"start": 819.84, "end": 820.06, "word": " الـ", "probability": 0.885498046875}, {"start": 820.06, "end": 820.36, "word": " Red", "probability": 0.57958984375}, {"start": 820.36, "end": 820.56, "word": " a", "probability": 0.12432861328125}, {"start": 820.56, "end": 821.06, "word": " phenolic", "probability": 0.879638671875}, {"start": 821.06, "end": 821.52, "word": " stain", "probability": 0.96630859375}, {"start": 821.52, "end": 821.9, "word": " لأنه", "probability": 0.8984375}, {"start": 821.9, "end": 822.22, "word": " أحد", "probability": 0.929931640625}, {"start": 822.22, "end": 823.08, "word": " مكونات", "probability": 0.94619140625}, {"start": 823.08, "end": 823.72, "word": " الصبغة", "probability": 0.949951171875}, {"start": 823.72, "end": 824.0, "word": " الـ", "probability": 0.751708984375}, {"start": 824.0, "end": 824.56, "word": " phenol", "probability": 0.6707763671875}, {"start": 824.56, "end": 825.2, "word": " is", "probability": 0.2080078125}, {"start": 825.2, "end": 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"end": 835.34, "word": " the", "probability": 0.84765625}, {"start": 835.34, "end": 836.44, "word": " mycobacterial", "probability": 0.877734375}, {"start": 836.44, "end": 836.82, "word": " cell", "probability": 0.92724609375}, {"start": 836.82, "end": 837.12, "word": " wall", "probability": 0.88134765625}, {"start": 837.12, "end": 837.64, "word": " بقولنا", "probability": 0.9132486979166666}, {"start": 837.64, "end": 837.92, "word": " هذه", "probability": 0.348388671875}, {"start": 837.92, "end": 838.64, "word": " الصبغة", "probability": 0.9920654296875}, {"start": 838.64, "end": 839.42, "word": " بتدوب", "probability": 0.8670247395833334}, {"start": 839.42, "end": 839.58, "word": " في", "probability": 0.97265625}, {"start": 839.58, "end": 840.0, "word": " الدهون", "probability": 0.97509765625}, {"start": 840.0, "end": 840.94, "word": " الموجودة", "probability": 0.9815673828125}, {"start": 840.94, "end": 841.44, "word": " على", "probability": 0.88427734375}, {"start": 841.44, "end": 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"end": 859.11, "word": " بيعطي", "probability": 0.9078369140625}, {"start": 859.11, "end": 859.45, "word": " اللون", "probability": 0.699951171875}, {"start": 859.45, "end": 860.11, "word": " on", "probability": 0.223876953125}, {"start": 860.11, "end": 860.73, "word": " carbolic", "probability": 0.76171875}, {"start": 860.73, "end": 861.17, "word": " acid", "probability": 0.93603515625}, {"start": 861.17, "end": 862.09, "word": " الفينول", "probability": 0.8146158854166666}, {"start": 862.09, "end": 862.83, "word": " الفينول", "probability": 0.9392903645833334}, {"start": 862.83, "end": 863.37, "word": " يعتبر", "probability": 0.95654296875}, {"start": 863.37, "end": 863.77, "word": " as", "probability": 0.8515625}, {"start": 863.77, "end": 864.45, "word": " mordant", "probability": 0.8015950520833334}, {"start": 864.45, "end": 864.77, "word": " مع", "probability": 0.92529296875}, {"start": 864.77, "end": 865.03, "word": " ال", "probability": 0.8623046875}, {"start": 865.03, "end": 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" اللي", "probability": 0.815185546875}, {"start": 1111.04, "end": 1111.24, "word": " هو", "probability": 0.92626953125}, {"start": 1111.24, "end": 1111.38, "word": " ال", "probability": 0.9462890625}, {"start": 1111.38, "end": 1112.24, "word": " decolorizer", "probability": 0.6671549479166666}, {"start": 1112.24, "end": 1112.68, "word": " لمدة", "probability": 0.904296875}, {"start": 1112.68, "end": 1113.0, "word": " خمس", "probability": 0.8087565104166666}, {"start": 1113.0, "end": 1113.38, "word": " عشر", "probability": 0.4383544921875}, {"start": 1113.38, "end": 1114.14, "word": " لعشرين", "probability": 0.814697265625}, {"start": 1114.14, "end": 1114.48, "word": " second", "probability": 0.55029296875}, {"start": 1114.48, "end": 1114.96, "word": " بعدها", "probability": 0.923095703125}, {"start": 1114.96, "end": 1115.34, "word": " بغسل", "probability": 0.9100341796875}, {"start": 1115.34, "end": 1115.54, "word": " بال", "probability": 0.97509765625}, {"start": 1115.54, "end": 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decolorizer احتفظت بلون ال primer stain الكربوفوكسينومائد حتى الحمض القوي يزيل اللون منها", "tokens": [3224, 1829, 8717, 2655, 16373, 7435, 8258, 2370, 16441, 9154, 15040, 5016, 19913, 28242, 31767, 13063, 39896, 8258, 2370, 4724, 9381, 15040, 1829, 39896, 4117, 4724, 4117, 2655, 3555, 28239, 31371, 7435, 3660, 8258, 2370, 4724, 9381, 15040, 1829, 4724, 9381, 15040, 1829, 25124, 3615, 3555, 6055, 3555, 3615, 11296, 4724, 9381, 15040, 1829, 6225, 9381, 45865, 4032, 8258, 2370, 37495, 22653, 12174, 995, 20498, 3660, 21542, 2304, 11242, 805, 4, 479, 22458, 979, 36182, 6545, 1975, 33753, 5172, 19913, 2655, 4724, 1211, 11536, 2423, 12595, 16441, 33251, 25513, 38688, 2407, 4117, 3794, 9957, 20498, 16373, 3215, 11331, 49975, 21542, 2304, 11242, 25062, 45865, 7251, 11622, 26895, 13672, 11536, 9154, 11296], "avg_logprob": -0.27483258422996315, "compression_ratio": 1.6899563318777293, "no_speech_prob": 2.384185791015625e-07, "words": [{"start": 1222.12, "end": 1222.42, "word": "هي", 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"word": " acid", "probability": 0.888671875}, {"start": 1234.5, "end": 1234.94, "word": " fast", "probability": 0.89208984375}, {"start": 1234.94, "end": 1235.36, "word": " يعني", "probability": 0.9501953125}, {"start": 1235.36, "end": 1236.04, "word": " قاومة", "probability": 0.82177734375}, {"start": 1236.04, "end": 1236.52, "word": " الحمض", "probability": 0.8937174479166666}, {"start": 1236.52, "end": 1237.14, "word": " 3", "probability": 0.39990234375}, {"start": 1237.14, "end": 1237.76, "word": "%", "probability": 0.96533203125}, {"start": 1237.76, "end": 1238.46, "word": " FCL", "probability": 0.3341064453125}, {"start": 1238.46, "end": 1239.58, "word": " decolorizer", "probability": 0.6086832682291666}, {"start": 1239.58, "end": 1240.74, "word": " احتفظت", "probability": 0.93271484375}, {"start": 1240.74, "end": 1241.2, "word": " بلون", "probability": 0.95654296875}, {"start": 1241.2, "end": 1241.46, "word": " ال", "probability": 0.93212890625}, {"start": 1241.46, "end": 1241.9, "word": " primer", "probability": 0.188232421875}, {"start": 1241.9, "end": 1242.36, "word": " stain", "probability": 0.8486328125}, {"start": 1242.36, "end": 1243.92, "word": " الكربوفوكسينومائد", "probability": 0.7000244140625}, {"start": 1243.92, "end": 1244.36, "word": " حتى", "probability": 0.724365234375}, {"start": 1244.36, "end": 1244.76, "word": " الحمض", "probability": 0.9806315104166666}, {"start": 1244.76, "end": 1245.3, "word": " القوي", "probability": 0.78271484375}, {"start": 1245.3, "end": 1246.22, "word": " يزيل", "probability": 0.9646809895833334}, {"start": 1246.22, "end": 1246.72, "word": " اللون", "probability": 0.989501953125}, {"start": 1246.72, "end": 1247.18, "word": " منها", "probability": 0.9716796875}], "temperature": 1.0}, {"id": 46, "seek": 127777, "start": 1251.33, "end": 1277.77, "text": "Cannon Stain or cold method method of staining acid fast microorganism هي طريقة لصباغة ال acid fast بسلعي خاصة الميكوبكتيريا procedure is similar to zel nelson stain الخطوات هي نفس خطوات ال zel nelson stain اللي هي ال acid fast stain but doesn't involve heating the slide لكن أنا ما بعرض ال slide ل steam عشان هيك سميتها بال cold method", "tokens": [34, 16138, 745, 491, 420, 3554, 3170, 3170, 295, 342, 3686, 8258, 2370, 49129, 1434, 39896, 23032, 16572, 28671, 5296, 9381, 3555, 5718, 118, 3660, 2423, 8258, 2370, 4724, 3794, 1211, 3615, 1829, 16490, 33546, 3660, 9673, 1829, 4117, 37746, 4117, 2655, 13546, 25528, 10747, 307, 2531, 281, 710, 338, 297, 20471, 16441, 33962, 9566, 2407, 9307, 39896, 8717, 36178, 16490, 9566, 2407, 9307, 2423, 710, 338, 297, 20471, 16441, 13672, 1829, 39896, 2423, 8258, 2370, 16441, 457, 1177, 380, 9494, 15082, 264, 4137, 44381, 41850, 19446, 45030, 43042, 2423, 4137, 5296, 11952, 6225, 8592, 7649, 39896, 4117, 8608, 2304, 36081, 11296, 20666, 3554, 3170], "avg_logprob": -0.27329009096577483, "compression_ratio": 1.6082089552238805, "no_speech_prob": 1.1920928955078125e-07, "words": [{"start": 1251.33, "end": 1251.79, 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1257.85, "word": " طريقة", "probability": 0.9908854166666666}, {"start": 1257.85, "end": 1258.57, "word": " لصباغة", "probability": 0.800537109375}, {"start": 1258.57, "end": 1258.73, "word": " ال", "probability": 0.9482421875}, {"start": 1258.73, "end": 1258.97, "word": " acid", "probability": 0.72509765625}, {"start": 1258.97, "end": 1259.43, "word": " fast", "probability": 0.84375}, {"start": 1259.43, "end": 1260.13, "word": " بسلعي", "probability": 0.506982421875}, {"start": 1260.13, "end": 1260.65, "word": " خاصة", "probability": 0.958984375}, {"start": 1260.65, "end": 1261.83, "word": " الميكوبكتيريا", "probability": 0.756011962890625}, {"start": 1261.83, "end": 1262.61, "word": " procedure", "probability": 0.654296875}, {"start": 1262.61, "end": 1262.91, "word": " is", "probability": 0.58349609375}, {"start": 1262.91, "end": 1263.35, "word": " similar", "probability": 0.9443359375}, {"start": 1263.35, "end": 1263.71, "word": " to", "probability": 0.95263671875}, {"start": 1263.71, "end": 1263.93, "word": " zel", "probability": 0.482666015625}, {"start": 1263.93, "end": 1264.27, "word": " nelson", "probability": 0.54742431640625}, {"start": 1264.27, "end": 1264.81, "word": " stain", "probability": 0.9033203125}, {"start": 1264.81, "end": 1265.71, "word": " الخطوات", "probability": 0.96044921875}, {"start": 1265.71, "end": 1265.89, "word": " هي", "probability": 0.8740234375}, {"start": 1265.89, "end": 1266.19, "word": " نفس", "probability": 0.993408203125}, {"start": 1266.19, "end": 1266.95, "word": " خطوات", "probability": 0.9737548828125}, {"start": 1266.95, "end": 1267.23, "word": " ال", "probability": 0.72314453125}, {"start": 1267.23, "end": 1267.47, "word": " zel", "probability": 0.798095703125}, {"start": 1267.47, "end": 1267.85, "word": " nelson", "probability": 0.935791015625}, {"start": 1267.85, "end": 1268.39, "word": " stain", "probability": 0.8955078125}, {"start": 1268.39, "end": 1268.57, "word": " اللي", "probability": 0.888671875}, {"start": 1268.57, "end": 1268.73, "word": " هي", "probability": 0.83251953125}, {"start": 1268.73, "end": 1268.91, "word": " ال", "probability": 0.88818359375}, {"start": 1268.91, "end": 1269.15, "word": " acid", "probability": 0.9833984375}, {"start": 1269.15, "end": 1269.61, "word": " fast", "probability": 0.88818359375}, {"start": 1269.61, "end": 1270.31, "word": " stain", "probability": 0.94775390625}, {"start": 1270.31, "end": 1270.85, "word": " but", "probability": 0.80712890625}, {"start": 1270.85, "end": 1271.33, "word": " doesn't", "probability": 0.83203125}, {"start": 1271.33, "end": 1271.93, "word": " involve", "probability": 0.88427734375}, {"start": 1271.93, "end": 1272.51, "word": " heating", "probability": 0.95947265625}, {"start": 1272.51, "end": 1272.81, "word": " the", "probability": 0.89599609375}, {"start": 1272.81, "end": 1273.15, "word": " slide", "probability": 0.89892578125}, {"start": 1273.15, "end": 1273.49, "word": " لكن", "probability": 0.9013671875}, {"start": 1273.49, "end": 1273.79, "word": " أنا", "probability": 0.75732421875}, {"start": 1273.79, "end": 1273.99, "word": " ما", "probability": 0.85595703125}, {"start": 1273.99, "end": 1274.61, "word": " بعرض", "probability": 0.9033203125}, {"start": 1274.61, "end": 1274.91, "word": " ال", "probability": 0.8759765625}, {"start": 1274.91, "end": 1275.39, "word": " slide", "probability": 0.9853515625}, {"start": 1275.39, "end": 1275.59, "word": " ل", "probability": 0.62255859375}, {"start": 1275.59, "end": 1275.89, "word": " steam", "probability": 0.1591796875}, {"start": 1275.89, "end": 1276.17, "word": " عشان", "probability": 0.9513346354166666}, {"start": 1276.17, "end": 1276.39, "word": " هيك", "probability": 0.77978515625}, {"start": 1276.39, "end": 1276.85, "word": " سميتها", "probability": 0.9339599609375}, {"start": 1276.85, "end": 1277.03, "word": " بال", "probability": 0.7685546875}, {"start": 1277.03, "end": 1277.37, "word": " cold", "probability": 0.896484375}, {"start": 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48, "seek": 132994, "start": 1306.88, "end": 1329.94, "text": "ما بحتاج الـ heat الحرارة حتى إنها تخترق الـ waxy label تحت المايكروسكوب بشوف الخلايا الـ acid-fast organism بتظهر باللون الأحمر و الـ non-acid-fast organism بتظهر باللون الأزرق نفس نتائج الـ acid-fastستانورامينرودامينستان", "tokens": [15042, 4724, 33753, 26108, 2423, 39184, 3738, 21542, 2288, 9640, 3660, 11331, 49975, 36145, 11296, 6055, 46456, 2288, 4587, 2423, 39184, 17352, 88, 7645, 6055, 33753, 9673, 47302, 4117, 32887, 3794, 4117, 37746, 4724, 8592, 38688, 33962, 15040, 25528, 2423, 39184, 8258, 12, 7011, 24128, 39894, 19913, 3224, 2288, 20666, 1211, 11536, 16247, 5016, 29973, 4032, 2423, 39184, 2107, 12, 326, 327, 12, 7011, 24128, 39894, 19913, 3224, 2288, 20666, 1211, 11536, 16247, 11622, 2288, 4587, 8717, 36178, 8717, 2655, 16373, 7435, 2423, 39184, 8258, 12, 7011, 14851, 7649, 13063, 10943, 9957, 2288, 23328, 10943, 9957, 14851, 7649], "avg_logprob": -0.20785984156107662, "compression_ratio": 1.7828282828282829, 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"probability": 0.9944661458333334}, {"start": 1350.55, "end": 1350.93, "word": " بقدر", "probability": 0.98388671875}, {"start": 1350.93, "end": 1351.27, "word": " أشوف", "probability": 0.95556640625}, {"start": 1351.27, "end": 1351.43, "word": " ال", "probability": 0.9755859375}, {"start": 1351.43, "end": 1351.67, "word": " acid", "probability": 0.990234375}, {"start": 1351.67, "end": 1352.11, "word": " fast", "probability": 0.8515625}, {"start": 1352.11, "end": 1352.77, "word": "-bacilli", "probability": 0.938720703125}, {"start": 1352.77, "end": 1353.93, "word": " باستخدام", "probability": 0.9844563802083334}, {"start": 1353.93, "end": 1354.09, "word": " ال", "probability": 0.273193359375}, {"start": 1354.09, "end": 1354.57, "word": " fluorescent", "probability": 0.8232421875}, {"start": 1354.57, "end": 1355.49, "word": " microscope", "probability": 0.87255859375}, {"start": 1355.49, "end": 1356.21, "word": " هتظهر", "probability": 0.9849609375}, {"start": 1356.21, "end": 1356.39, "word": " ال", "probability": 0.9580078125}, {"start": 1356.39, "end": 1357.21, "word": " mycobacterium", "probability": 0.95166015625}, {"start": 1357.21, "end": 1357.65, "word": " بلون", "probability": 0.9523111979166666}, {"start": 1357.65, "end": 1358.23, "word": " أصفر", "probability": 0.9400634765625}, {"start": 1358.23, "end": 1358.73, "word": " reddish", "probability": 0.786865234375}, {"start": 1358.73, "end": 1359.15, "word": " yellow", "probability": 0.6328125}], "temperature": 1.0}, {"id": 50, "seek": 138822, "start": 1362.74, "end": 1388.22, "text": "أخر معلومة في الـ Acid Fast StainThe reddish pink color and carding sticking together in long ruby masses of the mycobacterium cell due to the excess lipid of the cell wall بقولنا في انديانا طبقة شمعية بتخلي البكتيريا تلزق بعض بتكون مبينة على شكل الحبل carding تمام هذه الظاهرة اسمها الـ ruby masses أو ممكن نسميها carding", "tokens": [10721, 34740, 20449, 1211, 20498, 3660, 8978, 2423, 39184, 5097, 327, 15968, 745, 491, 2278, 2182, 40974, 7022, 2017, 293, 2920, 278, 13465, 1214, 294, 938, 5915, 88, 23935, 295, 264, 452, 66, 996, 14125, 2197, 2815, 3462, 281, 264, 9310, 8280, 327, 295, 264, 2815, 2929, 4724, 39648, 8315, 8978, 16472, 16254, 7649, 995, 23032, 3555, 28671, 13412, 2304, 3615, 10632, 39894, 9778, 20292, 29739, 4117, 2655, 13546, 25528, 6055, 1211, 11622, 4587, 45030, 11242, 39894, 30544, 3714, 3555, 9957, 3660, 15844, 13412, 28820, 21542, 36150, 2920, 278, 46811, 10943, 29538, 6024, 116, 40294, 25720, 24525, 2304, 11296, 2423, 39184, 5915, 88, 23935, 34051, 3714, 43020, 8717, 38251, 1829, 11296, 2920, 278], "avg_logprob": -0.22574012883399663, "compression_ratio": 1.508650519031142, "no_speech_prob": 5.364418029785156e-07, "words": [{"start": 1362.74, "end": 1363.14, "word": "أخر", "probability": 0.6724853515625}, {"start": 1363.14, "end": 1363.8, "word": " معلومة", "probability": 0.9903564453125}, {"start": 1363.8, "end": 1364.04, "word": " في", "probability": 0.958984375}, {"start": 1364.04, "end": 1364.3, "word": " الـ", "probability": 0.850341796875}, {"start": 1364.3, "end": 1364.62, "word": " Acid", "probability": 0.692626953125}, {"start": 1364.62, "end": 1365.0, "word": " Fast", "probability": 0.4208984375}, {"start": 1365.0, "end": 1366.24, "word": " StainThe", "probability": 0.42401123046875}, {"start": 1366.24, "end": 1366.68, "word": " reddish", "probability": 0.901611328125}, {"start": 1366.68, "end": 1366.98, "word": " pink", "probability": 0.53515625}, {"start": 1366.98, "end": 1367.44, "word": " color", "probability": 0.6962890625}, {"start": 1367.44, "end": 1368.06, "word": " and", "probability": 0.931640625}, {"start": 1368.06, "end": 1368.78, "word": " carding", "probability": 0.758544921875}, {"start": 1368.78, "end": 1369.32, "word": " sticking", "probability": 0.8349609375}, {"start": 1369.32, "end": 1369.86, "word": " together", "probability": 0.861328125}, {"start": 1369.86, "end": 1370.12, "word": " in", "probability": 0.9228515625}, {"start": 1370.12, "end": 1370.44, "word": " long", "probability": 0.9111328125}, {"start": 1370.44, "end": 1370.72, "word": " ruby", "probability": 0.7215576171875}, {"start": 1370.72, "end": 1371.06, "word": " masses", "probability": 0.89501953125}, {"start": 1371.06, "end": 1371.44, "word": " of", "probability": 0.921875}, {"start": 1371.44, "end": 1371.64, "word": " the", "probability": 0.90673828125}, {"start": 1371.64, "end": 1372.54, "word": " mycobacterium", "probability": 0.85732421875}, {"start": 1372.54, "end": 1372.84, "word": " cell", "probability": 0.87646484375}, {"start": 1372.84, "end": 1373.28, "word": " due", "probability": 0.87353515625}, {"start": 1373.28, "end": 1373.82, "word": " to", "probability": 0.630859375}, {"start": 1373.82, "end": 1373.88, "word": " the", "probability": 0.76513671875}, {"start": 1373.88, "end": 1374.32, "word": " excess", "probability": 0.91845703125}, {"start": 1374.32, "end": 1374.74, "word": " lipid", "probability": 0.730224609375}, {"start": 1374.74, "end": 1374.96, "word": " of", "probability": 0.8662109375}, {"start": 1374.96, "end": 1375.12, "word": " the", "probability": 0.87255859375}, {"start": 1375.12, "end": 1375.36, "word": " cell", "probability": 0.90673828125}, {"start": 1375.36, "end": 1375.6, "word": " wall", "probability": 0.91064453125}, {"start": 1375.6, "end": 1376.06, "word": " بقولنا", "probability": 0.8434244791666666}, {"start": 1376.06, "end": 1376.2, "word": " في", "probability": 0.88330078125}, {"start": 1376.2, "end": 1376.8, "word": " انديانا", "probability": 0.57806396484375}, {"start": 1376.8, "end": 1377.24, "word": " طبقة", "probability": 0.8821614583333334}, {"start": 1377.24, "end": 1377.84, "word": " شمعية", "probability": 0.9891357421875}, {"start": 1377.84, "end": 1378.34, "word": " بتخلي", "probability": 0.822265625}, {"start": 1378.34, "end": 1378.94, "word": " البكتيريا", "probability": 0.950390625}, {"start": 1378.94, "end": 1379.46, "word": " تلزق", "probability": 0.82958984375}, {"start": 1379.46, "end": 1379.88, "word": " بعض", "probability": 0.898193359375}, {"start": 1379.88, "end": 1380.26, "word": " بتكون", "probability": 0.8037109375}, {"start": 1380.26, "end": 1380.76, "word": " مبينة", "probability": 0.865234375}, {"start": 1380.76, "end": 1380.94, "word": " على", "probability": 0.8974609375}, {"start": 1380.94, "end": 1381.28, "word": " شكل", "probability": 0.9921875}, {"start": 1381.28, "end": 1381.74, "word": " الحبل", "probability": 0.993896484375}, {"start": 1381.74, "end": 1382.56, "word": " carding", "probability": 0.75}, {"start": 1382.56, "end": 1383.38, "word": " تمام", "probability": 0.885009765625}, {"start": 1383.38, "end": 1384.12, "word": " هذه", "probability": 0.53759765625}, {"start": 1384.12, "end": 1384.66, "word": " الظاهرة", "probability": 0.9488525390625}, {"start": 1384.66, "end": 1385.16, "word": " اسمها", "probability": 0.9597981770833334}, {"start": 1385.16, "end": 1385.5, "word": " الـ", "probability": 0.7225341796875}, {"start": 1385.5, "end": 1385.78, "word": " ruby", "probability": 0.634521484375}, {"start": 1385.78, "end": 1386.34, "word": " masses", "probability": 0.8837890625}, {"start": 1386.34, "end": 1386.6, "word": " أو", "probability": 0.80126953125}, {"start": 1386.6, "end": 1386.86, "word": " ممكن", "probability": 0.96923828125}, {"start": 1386.86, "end": 1387.5, "word": " نسميها", "probability": 0.9866943359375}, {"start": 1387.5, "end": 1388.22, "word": " carding", "probability": 0.643310546875}], "temperature": 1.0}, {"id": 51, "seek": 139541, "start": 1388.93, "end": 1395.41, "text": "هذه الظاهرة تكون في الـ Mycobacterium نتيجة الـ high concentration of lipid", "tokens": [3224, 24192, 6024, 116, 40294, 25720, 6055, 30544, 8978, 2423, 39184, 1222, 66, 996, 14125, 2197, 8717, 31371, 7435, 3660, 2423, 39184, 1090, 9856, 295, 8280, 327], "avg_logprob": -0.2823660575917789, "compression_ratio": 1.00990099009901, "no_speech_prob": 0.0, "words": [{"start": 1388.93, "end": 1389.35, "word": "هذه", "probability": 0.852294921875}, {"start": 1389.35, "end": 1389.87, "word": " الظاهرة", "probability": 0.97265625}, {"start": 1389.87, "end": 1390.41, "word": " تكون", "probability": 0.519287109375}, {"start": 1390.41, "end": 1390.73, "word": " في", "probability": 0.92431640625}, {"start": 1390.73, "end": 1391.19, "word": " الـ", "probability": 0.689208984375}, {"start": 1391.19, "end": 1392.11, "word": " Mycobacterium", "probability": 0.83896484375}, {"start": 1392.11, "end": 1392.91, "word": " نتيجة", "probability": 0.9478759765625}, {"start": 1392.91, "end": 1393.23, "word": " الـ", "probability": 0.762451171875}, {"start": 1393.23, "end": 1393.47, "word": " high", "probability": 0.38037109375}, {"start": 1393.47, "end": 1394.25, "word": " concentration", "probability": 0.8955078125}, {"start": 1394.25, "end": 1394.79, "word": " of", "probability": 0.9873046875}, {"start": 1394.79, "end": 1395.41, "word": " lipid", "probability": 0.36541748046875}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 1402.601375, "duration_after_vad": 1354.5974999999999}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/9AIy0KlGCSw_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/9AIy0KlGCSw_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..c826fa45f73dd222dceb5a85e38e99b7a7a33704
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/9AIy0KlGCSw_postprocess.srt
@@ -0,0 +1,1664 @@
+1
+00:00:00,720 --> 00:00:04,220
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:04,220 --> 00:00:07,780
+جميعا في هذا اللقاء هنتكلم عن ال bacterial
+
+3
+00:00:07,780 --> 00:00:12,700
+motility حركة البكتيريا البكتيريا بتتحرك بواسطة
+
+4
+00:00:12,700 --> 00:00:16,060
+الفلاجلة وعرفنا أن الفلاجلة يعتبر special
+
+5
+00:00:16,060 --> 00:00:19,360
+component يعني في بكتيريا عندي motile وفي بكتيريا
+
+6
+00:00:19,360 --> 00:00:24,120
+non motile عشان أعرف هل البكتيريا متحركة أو لأ في
+
+7
+00:00:24,120 --> 00:00:28,940
+عندي تلت طرق هنتعرف عليها في هذه المحاضرةأحد هذه
+
+8
+00:00:28,940 --> 00:00:33,300
+الطرق هي الـ Flagella Staining اللي يعتبر Special
+
+9
+00:00:33,300 --> 00:00:38,540
+Stain لو بدنا نعرف الـ Motility Motility The
+
+10
+00:00:38,540 --> 00:00:42,780
+ability of an organism to move by itself هو قدرة
+
+11
+00:00:42,780 --> 00:00:47,040
+ال micro organism أو البكتيريا على الحركة بنفسها
+
+12
+00:00:47,040 --> 00:00:52,640
+by itself هذا هو تعريف ال Motility قدرة ال micro
+
+13
+00:00:52,640 --> 00:00:55,660
+organism على الحركة بنفسها by itself
+
+14
+00:01:00,530 --> 00:01:05,030
+ليش by itself لأنه ممكن تتحرك by activity of water
+
+15
+00:01:05,030 --> 00:01:09,070
+molecule بواسطة حركة جزيئات الماء وهي تعتبر
+
+16
+00:01:09,070 --> 00:01:13,290
+Brownian movement لكن لو اتحركت البكتيريا by
+
+17
+00:01:13,290 --> 00:01:18,170
+itself بسميها ل true movement motility is closely
+
+18
+00:01:18,170 --> 00:01:21,810
+linked with chemotaxis مصطلح ال motility مرتبط
+
+19
+00:01:21,810 --> 00:01:26,190
+بمصطلح تاني اسمه ال chemotaxis لو بدنا نعرف ال
+
+20
+00:01:26,190 --> 00:01:30,310
+chemotaxisThe ability to oriented along certain
+
+21
+00:01:30,310 --> 00:01:34,910
+chemical gradient هي حركة البكتيريا حسب تركيز
+
+22
+00:01:34,910 --> 00:01:38,850
+المواد الكيميائية لو كان في عندي مغذيات أكيد
+
+23
+00:01:38,850 --> 00:01:43,150
+هتتحرك البكتيريا باتجاه هذه المغذيات لو كان في
+
+24
+00:01:43,150 --> 00:01:47,990
+عندي toxic هتتحرك بعيد عن ال toxic هتهرب منهاA
+
+25
+00:01:47,990 --> 00:01:51,590
+large number of bacteria are motile، أعداد كبيرة
+
+26
+00:01:51,590 --> 00:01:56,070
+من البكتيريا تعتبر motile، يبقى النسبة القليلة
+
+27
+00:01:56,070 --> 00:02:00,330
+يعتبر non-motile Most bosses one or more flagella
+
+28
+00:02:00,330 --> 00:02:04,310
+on their surface that allow them to swim البكتيريا
+
+29
+00:02:04,310 --> 00:02:08,790
+المتحركة بتتحرك بالفلاجلة بالأصوات، بتمتلك إما صوت
+
+30
+00:02:08,790 --> 00:02:13,630
+واحد أو مجموعة أصوات على السطح بيسمح إلها بالحركة
+
+31
+00:02:14,290 --> 00:02:17,990
+بكتيريا الفلاجلة لو بدنا نعرف الفلاجلة are tiny
+
+32
+00:02:17,990 --> 00:02:22,810
+hell-like organelles of locomotion هي عبارة عن
+
+33
+00:02:22,810 --> 00:02:28,970
+زواق دقيقة زي الشعر تستخدم في الحركة من وين بينشأ
+
+34
+00:02:28,970 --> 00:02:33,190
+الفلاجلة originating in the cytoplasm بينشأ من
+
+35
+00:02:33,190 --> 00:02:38,930
+الcytoplasm beneath the cell wall بيطلع الصوت خارج
+
+36
+00:02:38,930 --> 00:02:43,830
+ال cell wall they extend beyond the cellويمتد خارج
+
+37
+00:02:43,830 --> 00:02:48,910
+الخلية بقولي طول الصوت ممكن يكون بيساوي طول الخلية
+
+38
+00:02:48,910 --> 00:02:53,890
+البكتيرية او اطول منها بشوية their fine protein
+
+39
+00:02:53,890 --> 00:02:57,850
+structure عن اي protein بيقصد في هاي ال slide
+
+40
+00:02:57,850 --> 00:03:05,090
+بقولي ان الفلاجلة بتكون من protein بسميه الفلاجلين
+
+41
+00:03:05,090 --> 00:03:09,370
+هذا ال protein يعتبر fine خفيف مابقدر اشوفه
+
+42
+00:03:09,370 --> 00:03:13,250
+بالتالي محتاجة requireSpecialist Training
+
+43
+00:03:13,250 --> 00:03:17,710
+Technique محتاجة لصبغات خاصة for demonstrating
+
+44
+00:03:17,710 --> 00:03:21,410
+them with a light microscope عشان أقدر أشوفه تحت
+
+45
+00:03:21,410 --> 00:03:24,690
+ال microscope يبقى ال protein المقصود في هذه
+
+46
+00:03:24,690 --> 00:03:29,370
+الجملة الفلاجلين لأنه الفلاجلة بتتكون من ال
+
+47
+00:03:29,370 --> 00:03:31,150
+protein اسمه الفلاجلين
+
+48
+00:03:36,770 --> 00:03:41,210
+site of a flagella موقع الأصوات the pattern of
+
+49
+00:03:41,210 --> 00:03:44,370
+flagellation is an important feature in
+
+50
+00:03:44,370 --> 00:03:48,550
+identification of motile bacteria ولتوزيع الأصوات
+
+51
+00:03:48,550 --> 00:03:52,890
+للبكتيريا المتحركة مهم جدا في ال identification زي
+
+52
+00:03:52,890 --> 00:03:55,790
+ما قلنا في ال Indus Bar location أنه بيساعد في
+
+53
+00:03:55,790 --> 00:03:58,950
+تعريف البكتيريا كمان ال site of a flagella بيساعد
+
+54
+00:03:58,950 --> 00:04:03,370
+في ال identification لو كانت البكتيريا is a single
+
+55
+00:04:03,370 --> 00:04:08,280
+polar flagelliumبسمي البكتيريا mono-tracheas لو
+
+56
+00:04:08,280 --> 00:04:13,260
+كانت بتمتلك صوت واحد فقط أكيد هيكون على قطب واحد
+
+57
+00:04:13,260 --> 00:04:21,200
+بسمي البكتيريا mono-tracheas amphitracheas one or
+
+58
+00:04:21,200 --> 00:04:25,440
+more في الجلة at both poles لو كان في عندي صوت
+
+59
+00:04:25,440 --> 00:04:32,600
+واحد لكن على كلا القطبينصوت واحد على كيلة القطبين
+
+60
+00:04:32,600 --> 00:04:36,640
+بسميه Amphitrachias او مجموعة أصوات ممكن يكون
+
+61
+00:04:36,640 --> 00:04:41,340
+مجموعة أصوات على الكيلة القطبين بسميه
+
+62
+00:04:41,340 --> 00:04:45,660
+Amphitrachias law for trachias tufts of flagella
+
+63
+00:04:45,660 --> 00:04:49,440
+at one end pole لو كان في عندي حزمة tufts يعني
+
+64
+00:04:49,440 --> 00:04:54,040
+حزمة of flagella at one end pole لو كان في عندي
+
+65
+00:04:54,040 --> 00:04:59,180
+حزمة من الأصوات موجودة على قطب واحد فقط بسميها law
+
+66
+00:04:59,180 --> 00:05:03,090
+for trachiasزي هك فقط على قطب واحد مش زي الـ
+
+67
+00:05:03,090 --> 00:05:07,330
+Amphitracius على القطبين الـ Monotracius صوت على
+
+68
+00:05:07,330 --> 00:05:12,350
+قطب لكن الـ Lymphotracius مجموعة أصوات على قطب
+
+69
+00:05:12,350 --> 00:05:17,050
+واحد Peritracius لو كانت الأصوات تحيط في الخلية
+
+70
+00:05:17,050 --> 00:05:21,190
+البكتيرية زي ما أحنا شايفين بالصورة هذه الأصوات
+
+71
+00:05:21,190 --> 00:05:26,770
+بتحيط في الخلية البكتيرية بسميها Peritracius طيب
+
+72
+00:05:26,770 --> 00:05:30,780
+الـ Tracius يعني نفات الصفةnon-motile يعني ما
+
+73
+00:05:30,780 --> 00:05:34,460
+بتتحرك without إفلاجلة أكيد مش هيكون عندها إفلاجلة
+
+74
+00:05:34,460 --> 00:05:38,800
+بتكون attracted ما عندها أي إفلاجلة هذا بالنسبة لل
+
+75
+00:05:38,800 --> 00:05:42,820
+site of إفلاجلة قولنا إن ال site of إفلاجلة بيساعد
+
+76
+00:05:42,820 --> 00:05:46,320
+في ال identification كيف لو كان عندي أنا عملت
+
+77
+00:05:46,320 --> 00:05:50,200
+إجرام أسنان و طلعت النتيجة إجرام negative Bacilli
+
+78
+00:05:50,200 --> 00:05:53,700
+أكيد الإجرام negative Bacilli بتضم مجموعة كبيرة من
+
+79
+00:05:53,700 --> 00:05:57,740
+البكتيريا عملت مجموعة فحوصات بالنهاية وصلت إنه
+
+80
+00:05:58,000 --> 00:06:03,680
+البكتيريا إما X أو Y كانت XY motile لكن توزيع
+
+81
+00:06:03,680 --> 00:06:07,640
+الأصوات تبعهم مختلف من خلال الـ site of the
+
+82
+00:06:07,640 --> 00:06:17,440
+flagella بقدر أعمل identification most
+
+83
+00:06:17,440 --> 00:06:21,640
+motile bacteria move in a straight line for a
+
+84
+00:06:21,640 --> 00:06:25,240
+brief time بقولي معظم البكتيريا المتحركة بتتحرك
+
+85
+00:06:25,240 --> 00:06:30,290
+بخط مستقيم لفترةو جيزة then turn and randomly
+
+86
+00:06:30,290 --> 00:06:33,870
+change direction before swimming again بقولي بعدها
+
+87
+00:06:33,870 --> 00:06:38,470
+بتغير الاتجاه و بتتحرك بشكل عشوائي ال flagella
+
+88
+00:06:38,470 --> 00:06:42,530
+composed from بقولي من أش بتكون ال flagella بتكون
+
+89
+00:06:42,530 --> 00:06:46,970
+من filament composed of a protein called flagellin
+
+90
+00:06:46,970 --> 00:06:52,380
+قلنا على ال flagellinبتكون الفلاجلة من filament
+
+91
+00:06:52,380 --> 00:06:56,400
+هذا ال filament بتكون من ال protein الفلاجلين يبقى
+
+92
+00:06:56,400 --> 00:07:01,880
+الفلاجلين a protein that composed filament ال hook
+
+93
+00:07:01,880 --> 00:07:05,400
+الخطاف base of filament near the cell wall هو
+
+94
+00:07:05,400 --> 00:07:10,360
+قاعدة ال filament تكون قريب من ال cell wallبازل
+
+95
+00:07:10,360 --> 00:07:14,240
+باضى آخر مكون البازل باضى بيقول لي بيربط انكار
+
+96
+00:07:14,240 --> 00:07:18,040
+بيربط او بيدعم ال filament و ال hook to the cell
+
+97
+00:07:18,040 --> 00:07:22,600
+wall بيربط و بيدعم ال filament و ال hook بال cell
+
+98
+00:07:22,600 --> 00:07:28,880
+wall فى عندي رسمة هى ال filament اللى قولنا بتكون
+
+99
+00:07:28,880 --> 00:07:32,580
+من بروتين لفلاجلين فى عندي ال hook الخطافة هو
+
+100
+00:07:32,580 --> 00:07:36,920
+قاعدة ال filament و فى عندي البازل باضى اللى بيربط
+
+101
+00:07:36,920 --> 00:07:42,460
+ال hook و ال filament بال cell walltype of
+
+102
+00:07:42,460 --> 00:07:47,840
+movement ممكن تكون عندي run run straight line
+
+103
+00:07:47,840 --> 00:07:50,780
+movement occur when the flagella rotate counter
+
+104
+00:07:50,780 --> 00:07:54,720
+clockwise البكتيريا بتتحرك بنوعين من الحركة إما
+
+105
+00:07:54,720 --> 00:08:00,740
+run تتحرك عكس عقارب الساعة tumble اللي هي turning
+
+106
+00:08:00,740 --> 00:08:03,960
+the direction by clockwise movement of the
+
+107
+00:08:03,960 --> 00:08:09,530
+flagella بتكون حركة عشوائية و مع عقارب الساعةهذا
+
+108
+00:08:09,530 --> 00:08:15,050
+بالنسبة للـ type of movement هنا
+
+109
+00:08:15,050 --> 00:08:20,370
+في السلايدات ذا كل ال site of flagella قلنا لو
+
+110
+00:08:20,370 --> 00:08:24,910
+كانت بتحيط بالخلية البكتيرية بسمي البكتيريا
+
+111
+00:08:24,910 --> 00:08:30,470
+periatriace من الأمثلة عليها ال E.coli نحفظ المثال
+
+112
+00:08:30,470 --> 00:08:34,690
+E.coli periatriace الفلاجلة بتحيط بالخلية
+
+113
+00:08:34,690 --> 00:08:41,230
+البكتيريةصوت واحد على قطب واحد mono-tracheas مثال
+
+114
+00:08:41,230 --> 00:08:47,530
+عليها الـ Vibrio cholera هنا عندي أنا حزمة من
+
+115
+00:08:47,530 --> 00:08:52,850
+الأصوات على صوت واحد بسميها الـ Lo-Fo Tracheas ذا
+
+116
+00:08:52,850 --> 00:08:58,030
+كل مثال الـ Sodomonas نحذف هذا ال Sodomonas هي
+
+117
+00:08:58,030 --> 00:09:03,550
+تعتبر mono-tracheas وليس Lo-Fo Tracheasيبقى نعدل
+
+118
+00:09:03,550 --> 00:09:06,610
+على ال slide أن الـ Sodomonas لا تعتبر Loft
+
+119
+00:09:06,610 --> 00:09:11,070
+Tracheas تعتبر Monotracheas يبقى Loft Tracheas لما
+
+120
+00:09:11,070 --> 00:09:15,270
+يكون في عندي حزمة من الأصوات على قطب واحد لما يكون
+
+121
+00:09:15,270 --> 00:09:19,470
+في عندي one or more flagella at both poles لما
+
+122
+00:09:19,470 --> 00:09:22,330
+يكون في عندي صوت واحد أو مجموعة أصوات على كلا
+
+123
+00:09:22,330 --> 00:09:26,410
+القطبين بسميها Amphitracheas مثال عليها الـ
+
+124
+00:09:26,410 --> 00:09:30,230
+Spirulum والتانستمام يبقى هدول كل الأمثلة اللي
+
+125
+00:09:30,230 --> 00:09:34,270
+ذكرناها يعتبروا motile لكن ال site of flagella
+
+126
+00:09:34,270 --> 00:09:43,750
+بيختلف motility
+
+127
+00:09:43,750 --> 00:09:49,430
+testing motility called by بيقوللي ممكن أنا أكشف
+
+128
+00:09:49,430 --> 00:09:53,310
+عن ال motility عن البكتيريا ال motility في تلت طرق
+
+129
+00:09:53,310 --> 00:09:58,340
+عشان أعرف هل البكتيريا motile or non motileالطريقة
+
+130
+00:09:58,340 --> 00:10:02,460
+الأولى الـ Hanging Drop Technique طريقة تانية
+
+131
+00:10:02,460 --> 00:10:06,280
+لفلاجلة استان اللى يعتبر special استان الطريقة
+
+132
+00:10:06,280 --> 00:10:10,040
+التالتة و اللى هى most common semisolid media
+
+133
+00:10:10,040 --> 00:10:15,580
+inculation أول طريقة اللى هى ال hanging drop slide
+
+134
+00:10:15,580 --> 00:10:20,860
+hanging معناها معلقة هى تعتبر الطريقة زى ال wet
+
+135
+00:10:20,860 --> 00:10:24,140
+mouth اللى قلنا من خلالها بقدر اتعرف على ال
+
+136
+00:10:24,140 --> 00:10:28,450
+bacterial morphology قلناالـ wet mouth بقدر اشوف
+
+137
+00:10:28,450 --> 00:10:33,770
+living bacteria living حية يعني بقدر اشوفها
+
+138
+00:10:33,770 --> 00:10:38,350
+ابتتحرك يعني هشوف حركة البكتيريا لكن انا لو صبغت
+
+139
+00:10:38,350 --> 00:10:42,070
+مش هشوف ال motility مش هشوف البكتيريا ابتتحرك لأن
+
+140
+00:10:42,070 --> 00:10:44,670
+احنا قولنا من خطوات الصباغة في ال heat في اندي
+
+141
+00:10:44,670 --> 00:10:50,650
+heat fixation بيعمل كلب يوقف ال movementفي الـ wet
+
+142
+00:10:50,650 --> 00:10:53,370
+mouth العادية اللي شرحناها قولنا إنه إحنا بنجيب
+
+143
+00:10:53,370 --> 00:10:57,990
+slide بنحط drop of bacterial suspension ومنحط فوق
+
+144
+00:10:57,990 --> 00:11:02,030
+cover slip هذه الطريقة ال wet mouth العادية اللي
+
+145
+00:11:02,030 --> 00:11:06,550
+شرحناها بيقولنا إنها بتشكل ضغط على ال slide فبتعيق
+
+146
+00:11:06,550 --> 00:11:10,090
+حركة البكتيريا فممكن البكتيريا تكون multi لكن
+
+147
+00:11:10,090 --> 00:11:16,860
+الضغط اللي شكله cover slip على ال slide هيعيق حركة
+
+148
+00:11:16,860 --> 00:11:21,840
+البكتيريا فبالتالي ممكن اشوفها non motile غير انه
+
+149
+00:11:21,840 --> 00:11:26,460
+في هذه الطريقة مش هقدر اميز ل true movement حركة
+
+150
+00:11:26,460 --> 00:11:31,780
+البكتيريا by itself by فلاجلة عن حركة البكتيريا ال
+
+151
+00:11:31,780 --> 00:11:36,280
+brownian movement اللي هي حركة جزيئات السائل يبقى
+
+152
+00:11:36,280 --> 00:11:39,600
+في ال wet mouth العادية لما اجيب slide و احط
+
+153
+00:11:39,600 --> 00:11:44,030
+bacterial suspension و cover slipهيضحيق حركة
+
+154
+00:11:44,030 --> 00:11:48,810
+البكتيريا لأنها تشكل ضغط تاني إشي مش هقدر أميز ال
+
+155
+00:11:48,810 --> 00:11:52,790
+true movement عن ال brownian movement عشان هيك
+
+156
+00:11:52,790 --> 00:11:56,830
+عملنا modification لنا ها لهذه الطريقة عشان أقدر
+
+157
+00:11:56,830 --> 00:12:01,350
+أحدد هل البكتيريا motile بتتحرك by itself أو non
+
+158
+00:12:01,350 --> 00:12:06,630
+motile الطريقة اسمها ال hanging drop slide بستخدم
+
+159
+00:12:06,630 --> 00:12:11,330
+slide بتتعلق فيها البكتيريا اسمها depression slide
+
+160
+00:12:12,340 --> 00:12:15,740
+ماهي الـ depression slide بقول هي slide that has a
+
+161
+00:12:15,740 --> 00:12:19,820
+concavity يعني إلها تقعر زي ما ملاحظين هي ال
+
+162
+00:12:19,820 --> 00:12:25,100
+depression slide في إلها بيكون فيه تقعر هاي ال
+
+163
+00:12:25,100 --> 00:12:30,860
+slide نكمل هي slide that has a concavity and cover
+
+164
+00:12:30,860 --> 00:12:34,820
+slip can be placed on it يعني ممكن كمان أني أنا
+
+165
+00:12:34,820 --> 00:12:38,740
+أحط ال cover slip فوق عليها يبقى هاي هي ال
+
+166
+00:12:38,740 --> 00:12:42,890
+depression slideبجيب drop of bacteria و بحطها على
+
+167
+00:12:42,890 --> 00:12:46,310
+cover slab وبعدها بقلبها و بحطها فوق depression
+
+168
+00:12:46,310 --> 00:12:51,150
+slide هيك بتتعلق البكتيريا طيب اتعلقت طبعا
+
+169
+00:12:51,150 --> 00:12:55,590
+البكتيريا على السطح هيك بقدر بكل سهولة احدد حركة
+
+170
+00:12:55,590 --> 00:12:59,750
+البكتيريا طيب لو كان ماعندي انا depression slide و
+
+171
+00:12:59,750 --> 00:13:03,070
+بدي اشوف البكتيريا ال mutilative طبعا هي slide
+
+172
+00:13:03,070 --> 00:13:06,970
+خاصة اسمها depression slide بكون فيه تقاعرطب انا
+
+173
+00:13:06,970 --> 00:13:10,010
+ماعندي هاي ال slide ممكن اقدر احدد ال bacterial
+
+174
+00:13:10,010 --> 00:13:13,870
+motility اقدر اعمل اشي اعمل زي ال depression slide
+
+175
+00:13:13,870 --> 00:13:19,270
+بقولنا اه بتجيب slide عادية تمام بتجيب slide عادية
+
+176
+00:13:19,270 --> 00:13:24,150
+و بجيب ال parafilm ال parafilm هو ورق بمغطب
+
+177
+00:13:24,150 --> 00:13:27,930
+نستخدمه أحيانا فيه عشان نسكر ال tube هذا ال
+
+178
+00:13:27,930 --> 00:13:32,950
+parafilm بروح انا بلف ال parafilm بجدل ال parafilm
+
+179
+00:13:32,950 --> 00:13:39,310
+و بعمل دائرة زي دائرةطبعا هي هتلذق على ال slide
+
+180
+00:13:39,310 --> 00:13:43,190
+باجي على نص ال slide هيك بيصير انا عندي concave
+
+181
+00:13:43,190 --> 00:13:49,450
+والسطح مقعر بجيب ال cover slip cover slip عادي بحط
+
+182
+00:13:49,450 --> 00:13:52,790
+drop of بكتيريا و بقلبها و بحطها فوق ال concave
+
+183
+00:13:52,790 --> 00:13:56,610
+والhigh اللي هي ال barifilm اللي أنا لفتها هيك انا
+
+184
+00:13:56,610 --> 00:14:01,190
+حصلت على depression slide صارت البكتيريا معلقة ما
+
+185
+00:14:01,190 --> 00:14:05,700
+بين ال cover slip و ما بين ال slideيبقى نقرا the
+
+186
+00:14:05,700 --> 00:14:09,820
+slide depression slide for hanging drop تستخدم ال
+
+187
+00:14:09,820 --> 00:14:13,940
+depression slide for hanging drop is a ground with
+
+188
+00:14:13,940 --> 00:14:19,260
+a concave well بقوللي فيها حفرة او فتحة مقعرة in
+
+189
+00:14:19,260 --> 00:14:21,440
+the center زي ما احنا شايفين ال depression slide
+
+190
+00:14:21,440 --> 00:14:25,800
+في عيني انا فتحة concave well فتحة مقعرة في
+
+191
+00:14:25,800 --> 00:14:30,280
+المنتصف the caverous glass hold a drop of the
+
+192
+00:14:30,280 --> 00:14:34,100
+suspension قولنا ال cover glass او ال cover slip
+
+193
+00:14:34,440 --> 00:14:37,520
+منحط عليه اللي هو ال bacterial suspension اللي هو
+
+194
+00:14:37,520 --> 00:14:42,840
+drop of bacteria when the gaffer when the gaffer
+
+195
+00:14:42,840 --> 00:14:47,160
+class is inverted over the well of the slide بقولي
+
+196
+00:14:47,160 --> 00:14:51,140
+لما أنا ال gaffer slip ها ده لبع على ال well هاي
+
+197
+00:14:51,140 --> 00:14:55,200
+اللي موجودة على ال slide the drop hangs from the
+
+198
+00:14:55,200 --> 00:15:00,060
+class in the hollow concavity of the slide بيصير
+
+199
+00:15:00,060 --> 00:15:03,650
+ال drop ofبكتيريا اللي كانت على ال cover slip
+
+200
+00:15:03,650 --> 00:15:08,350
+معلقة في ال concave well since the drop lies
+
+201
+00:15:08,350 --> 00:15:14,010
+within an enclosed glass chamber بقولنا عشان انا
+
+202
+00:15:14,010 --> 00:15:19,810
+احفظ ممكن احنا نحط اللي هو ال vaseline ال vaseline
+
+203
+00:15:19,810 --> 00:15:24,290
+تمام هذا ال vaseline ممكن انا احطه على حوالين ال
+
+204
+00:15:24,290 --> 00:15:31,350
+cover slip عشان يمنع الجفاف from drying outعشان
+
+205
+00:15:31,350 --> 00:15:38,150
+يمنع ممكن
+
+206
+00:15:38,150 --> 00:15:42,250
+نحط Vaseline حوالين اللي هو around the edge of the
+
+207
+00:15:42,250 --> 00:15:46,150
+cover slip حوالين ال cover slip هيحفظ ليها من
+
+208
+00:15:46,150 --> 00:15:49,730
+الجفاف ليش لأنه لو جفت البكتيريا مش هتقدر تتحرك
+
+209
+00:15:49,730 --> 00:15:53,830
+هتموت مش هتتحرك مش هقدر احدد هل البكتيريا motile
+
+210
+00:15:53,830 --> 00:15:57,290
+او non motile يبقى بعد ما احط ال cover slip علىالـ
+
+211
+00:15:57,290 --> 00:16:00,330
+depression slide أو على اللي انا حطيته هنا الـ
+
+212
+00:16:00,330 --> 00:16:04,850
+parafilm بحط Vaseline حوالين الـ cover slip هيك
+
+213
+00:16:04,850 --> 00:16:11,970
+هيحميلي البكتيريا من الجفا هذا بالنسبة للـ hanging
+
+214
+00:16:11,970 --> 00:16:15,390
+drop slide طبعا انا البكتيريا الـ bacteria
+
+215
+00:16:15,390 --> 00:16:23,260
+motility هتشوفها في hand على ال edge علىالـ edge
+
+216
+00:16:23,260 --> 00:16:27,120
+من اللي هو الـ concave well أو اللي هو اللي حطيته
+
+217
+00:16:27,120 --> 00:16:32,180
+البارع فيلم بقول
+
+218
+00:16:32,180 --> 00:16:35,700
+لي notes ملاحظة you should to be able to
+
+219
+00:16:35,700 --> 00:16:40,160
+differentiate true movement from Brownian movement
+
+220
+00:16:40,160 --> 00:16:43,240
+or motility يبقى احنا قلنا انه في عندي فرق بين ال
+
+221
+00:16:43,240 --> 00:16:46,840
+true movement و ال brownian movement ال brownian
+
+222
+00:16:46,840 --> 00:16:50,200
+movement بقول لي هي حركة جزيئات السائل activity of
+
+223
+00:16:50,200 --> 00:16:54,370
+water moleculeلكن بالنسبة لـ Retromovement اللي هي
+
+224
+00:16:54,370 --> 00:16:59,130
+حركة البكتيريا نفسها runs and tumble اللي هي تعتبر
+
+225
+00:16:59,130 --> 00:17:09,310
+حركة البكتيريا by itself by فلاجلة قلنا
+
+226
+00:17:09,310 --> 00:17:13,510
+عندي تلت طرق عشان أعرف هل البكتيريا motile أو non
+
+227
+00:17:13,510 --> 00:17:16,430
+-motile الطريقة الأولى اللي هي hanging drop
+
+228
+00:17:16,430 --> 00:17:20,280
+technique الطريقة التانية لفلاجلة استينةطريقة
+
+229
+00:17:20,280 --> 00:17:23,140
+تانية عشان أعرف هل البكتيريا non-motile أو non
+
+230
+00:17:23,140 --> 00:17:28,220
+-motile بدي أصبغ الفلاجلة المسؤول عن الحركة
+
+231
+00:17:28,220 --> 00:17:34,100
+الفلاجلة قولنا هو تنيق ضعيف خفيف لازم أنا أصبغه
+
+232
+00:17:34,100 --> 00:17:37,740
+عشان أقدر أشوفه flagella should be amplified
+
+233
+00:17:37,740 --> 00:17:41,400
+enlarged لازم أني أنا أكبره use a standard that is
+
+234
+00:17:41,400 --> 00:17:47,810
+especially deposit on flagellaincreasing diameter
+
+235
+00:17:47,810 --> 00:17:52,230
+بقول لي انا لما استخدم لستين هي الصبغة هتترسب على
+
+236
+00:17:52,230 --> 00:17:55,650
+ال flagella بالتالي هيزيد قطر ال flagella و اقدر
+
+237
+00:17:55,650 --> 00:18:00,730
+اني انا اشوفه some
+
+238
+00:18:00,730 --> 00:18:05,170
+في اندي انا طريقتين للصباغة قبل ما نحكي على
+
+239
+00:18:05,170 --> 00:18:09,250
+الطريقتين كأي خطوة من خطوات الصباغة هعمل smearing
+
+240
+00:18:09,250 --> 00:18:13,490
+preparation air drying لكن ال fixation هعمل
+
+241
+00:18:13,490 --> 00:18:19,860
+formalin fixationformalin fixation هعمل مش هي
+
+242
+00:18:19,860 --> 00:18:25,060
+fixation تمام رابع خطوة لستان اللي قولنا بتختلف
+
+243
+00:18:25,060 --> 00:18:28,180
+حسب نوع الاستان بالنسبة لفلاجلة استان في عندي
+
+244
+00:18:28,180 --> 00:18:32,020
+طريقتين الطريقة الأولى بستخدم ال primary استان
+
+245
+00:18:32,020 --> 00:18:37,360
+rosanilin-di يبقى ال primary استان هي rosanilin-di
+
+246
+00:18:37,360 --> 00:18:39,000
+بقولي بستخدمها
+
+247
+00:18:46,070 --> 00:18:49,730
+الـ rose-aniline dye هي الـ primary stain وبستخدم
+
+248
+00:18:49,730 --> 00:18:55,030
+أي ماردانت تمام بنفس الطريقة عملت سميري ب
+
+249
+00:18:55,030 --> 00:18:59,990
+preparation اير دراينج فورمالين فكساشن بعدها غمرت
+
+250
+00:18:59,990 --> 00:19:04,370
+ال slide بالـ rose-aniline dye اللي هي ال primary
+
+251
+00:19:04,370 --> 00:19:09,290
+stain عملت washing حطيت ماردانت تمام بعد هيك أنا
+
+252
+00:19:09,290 --> 00:19:13,750
+بقدر احدد اللي هي اشوف تحت ال microscope اللي هو
+
+253
+00:19:13,750 --> 00:19:18,830
+ال flagella هشوفهطبعاً بشكل واضح وكانتني لكن أنا
+
+254
+00:19:18,830 --> 00:19:22,190
+بعد ما صبغت الصبغة هتترسب على الـ Flagella هقدر
+
+255
+00:19:22,190 --> 00:19:26,690
+اني انا اشوفه Apply to bacterial suspension fixed
+
+256
+00:19:26,690 --> 00:19:31,150
+in formalin طبعا احنا من حط الـ Rosanilin dye and
+
+257
+00:19:31,150 --> 00:19:35,650
+المردن لبكتيريا ال suspension اللي انا تبته مسبقا
+
+258
+00:19:35,650 --> 00:19:39,710
+بال Formalin and spread across a glass slide
+
+259
+00:19:39,710 --> 00:19:44,790
+بقوللي ال Formalin linked to or fixed the flagella
+
+260
+00:19:45,300 --> 00:19:48,260
+أيش وظيفة ال Formalin اللي هو بيعمل fixation
+
+261
+00:19:48,260 --> 00:19:52,820
+لفلاجلة and other surface proteins of the cell The
+
+262
+00:19:52,820 --> 00:19:56,120
+dye اللي هي ال rose aniline dye and mordant then
+
+263
+00:19:56,120 --> 00:20:01,160
+precipitate around this fixed surface بقولي الصبغة
+
+264
+00:20:01,160 --> 00:20:05,330
+و ال mordant هيترسبواحوالين اللي هو الـ Flagella
+
+265
+00:20:05,330 --> 00:20:10,750
+اللي تم تثبيته بالـ Formalin Enlarging their
+
+266
+00:20:10,750 --> 00:20:14,750
+diameter، هيزيد حجم اللي هو الـ Diameter تبع الـ
+
+267
+00:20:14,750 --> 00:20:18,050
+Flagella and making Flagella visible when viewed
+
+268
+00:20:18,050 --> 00:20:23,030
+under the microscope، هيك أنا هقدر أني أشوفه تحت
+
+269
+00:20:23,030 --> 00:20:27,250
+الـ Microscopeبالنسبة للصبغة الروز أنالين داي
+
+270
+00:20:27,250 --> 00:20:31,790
+بتعطي اللون الأحمر راح يترسب حول الفلاجلة اللى تم
+
+271
+00:20:31,790 --> 00:20:34,870
+تثبيته بالفرمالين بالتالي هيك هيزيد حجمه هذه
+
+272
+00:20:34,870 --> 00:20:39,350
+بالنسبة للطريقة الأولى الطريقة التانية في الصباغة
+
+273
+00:20:39,350 --> 00:20:43,430
+في الفلاجلة الستان بقوللي بستخدم ال silver nitrate
+
+274
+00:20:43,430 --> 00:20:47,290
+كprimer استان وفرقتنات موردان بستخدم موردان
+
+275
+00:20:47,290 --> 00:20:51,590
+تلفرقتنات و ال silver nitrate يبقى بعمل smear
+
+276
+00:20:51,590 --> 00:20:56,570
+preparation air dryingfor maline fixation الصبغة
+
+277
+00:20:56,570 --> 00:21:01,010
+سيلفر نيترات هتعطي لون أسود والموردانت فى
+
+278
+00:21:01,010 --> 00:21:05,130
+الريكدانات موردانت بقولى هذا الصبغة مع الموردانت
+
+279
+00:21:05,130 --> 00:21:09,090
+هيتم تعريضها للبكتيريا ال suspension النتيجة
+
+280
+00:21:09,090 --> 00:21:12,710
+هيتكون dark precipitates that form on the bacteria
+
+281
+00:21:12,710 --> 00:21:16,010
+and their and their flagella allow them to be
+
+282
+00:21:16,010 --> 00:21:20,020
+easilyهقدر اني انا اشوفها تحت الماكروسكوب بكل
+
+283
+00:21:20,020 --> 00:21:23,400
+سهولة الصبغة وال موردانت هيترسبوا على الفلاجلة
+
+284
+00:21:23,400 --> 00:21:27,160
+فبالتالي هيزيد ال diameter للفلاجلة هقدر اني انا
+
+285
+00:21:27,160 --> 00:21:31,220
+اشوفها Silver Nitrate قولنا بيعطي اللوان الأسود
+
+286
+00:21:31,220 --> 00:21:35,460
+Silver Blatting Technique هاي الطريقة بستخدمها
+
+287
+00:21:35,460 --> 00:21:39,860
+used to stain the very slender spirot sheet بقوللي
+
+288
+00:21:39,860 --> 00:21:43,260
+هاي الطريقة انا بستخدمها في بكتيريا لصباغة هذه
+
+289
+00:21:43,260 --> 00:21:48,880
+البكتيريا very slenderSpiroid Sheet بكتيريا ماخدة
+
+290
+00:21:48,880 --> 00:21:52,860
+شكل الـ Spiral هاي الطريقة اللي هي الـ Silver
+
+291
+00:21:52,860 --> 00:21:57,360
+Nitrate و الفرقتانات Mordant يبقى في هاي الطريقة
+
+292
+00:21:57,360 --> 00:22:00,840
+بستخدم الـ Silver Nitrate ك Primary Stain و
+
+293
+00:22:00,840 --> 00:22:04,880
+Mordant الفرقتانات هضيفهم للـ Bacterial Suspension
+
+294
+00:22:04,880 --> 00:22:10,440
+هيترسبوا حول الفلاجلة وبالتالي هيعطي اللون الأسود
+
+295
+00:22:10,440 --> 00:22:14,770
+بالتالي سهل ان انا اشوفهم تحت ال microscopeهذه
+
+296
+00:22:14,770 --> 00:22:19,390
+الطريقة التانية ملاحظين في الصورة الفلاجولة اقدرت
+
+297
+00:22:19,390 --> 00:22:23,650
+اني انا اشوفه هي مسبوخ بالروزانالين داي اللي بتعطي
+
+298
+00:22:23,650 --> 00:22:28,690
+اللون الزهري لكن بالنسبة لهذه الصورة المسبوخ بالـ
+
+299
+00:22:28,690 --> 00:22:33,070
+silver nitrate زائد الفيرركتانات هيعطي اللون
+
+300
+00:22:33,070 --> 00:22:38,230
+الأسود آخر طريقة في اللي هو ال bacterial motility
+
+301
+00:22:38,230 --> 00:22:42,630
+اللي هي semi-solid media inculationاللي هي الـ
+
+302
+00:22:42,630 --> 00:22:46,130
+most common the most commonly used test for
+
+303
+00:22:46,130 --> 00:22:49,770
+motility in microbiology لأ هي أكثرها شيوعا
+
+304
+00:22:49,770 --> 00:22:53,890
+واستخداما في معمل الـ microbiology عشان أحدد هل
+
+305
+00:22:53,890 --> 00:22:58,410
+البكتيريا motile or non-motile بقوللي it depends
+
+306
+00:22:58,410 --> 00:23:01,850
+on the ability of motile bacteria to move through
+
+307
+00:23:01,850 --> 00:23:06,570
+semi-solid media هي بتعتمد على قدرة المكتيريا على
+
+308
+00:23:06,570 --> 00:23:11,770
+الحركة في semi-solid mediaالميديا قلنا هي وسط غضاء
+
+309
+00:23:11,770 --> 00:23:16,950
+لنمو البكتيريا بقسم الميديا حسب نسبة الأجار طبعا
+
+310
+00:23:16,950 --> 00:23:20,650
+الأجار اللي يعتبر هو solidifying agent اللي هو
+
+311
+00:23:20,650 --> 00:23:27,930
+بيخل الوسط صلب ممكن الميديا تكون solid و ممكن تكون
+
+312
+00:23:27,930 --> 00:23:32,680
+semi solid و ممكن تكون liquidلما تكون solid بتكون
+
+313
+00:23:32,680 --> 00:23:37,920
+نسبة الأجار من 1.5 لـ 2% أجار لو كانت semi-solid
+
+314
+00:23:37,920 --> 00:23:41,460
+اللي أنا بستخدمها في فحص الـ Motility نسبة الأجار
+
+315
+00:23:41,460 --> 00:23:48,380
+4 من 10% أجار لكن بالنسبة لو كانت liquid أكيد
+
+316
+00:23:48,380 --> 00:23:53,920
+هتكون no أجار لأنه ما صارش هي broth ضلت سائلة ما
+
+317
+00:23:53,920 --> 00:23:58,180
+اتصلبت يبقى توزيع أو تقسيم الـ media على حسب اللي
+
+318
+00:23:58,180 --> 00:24:03,490
+هو نسبة الأجار solidتكون نسبة الأجار من 1.5% لـ 2%
+
+319
+00:24:03,490 --> 00:24:09,550
+سيمي سوليد 4 من 10% Liquid مافيش أي نسبة أجار Zero
+
+320
+00:24:09,550 --> 00:24:16,710
+كيف أنا بدي أعمل الفحص قبل ما نحكي على كيف أنا ال
+
+321
+00:24:16,710 --> 00:24:19,890
+procedure لهذا الفحص فحص ال modality لازم يكون
+
+322
+00:24:19,890 --> 00:24:24,970
+عندي أنا سيمي سوليد 100 يعني نسبة الأجار 4 من 10%
+
+323
+00:24:24,970 --> 00:24:29,520
+لأنه أنا لو استخدمتsolid media أكيد البكتيريا مش
+
+324
+00:24:29,520 --> 00:24:34,240
+هتتحرك هيكون صلب لو استخدمت liquid هتتحرك وتنتشر
+
+325
+00:24:34,240 --> 00:24:38,460
+في كل tube ومش هعرف هل هي true movement او
+
+326
+00:24:38,460 --> 00:24:42,520
+Brownian movement اللي هي حركة جزيئات السائل فلازم
+
+327
+00:24:42,520 --> 00:24:47,440
+انا استخدم semi solid mediaطبعاً أنا هاي الـ Semi
+
+328
+00:24:47,440 --> 00:24:51,780
+-solid media بصوبها في تيوب تمام احنا قلنا نحضر ال
+
+329
+00:24:51,780 --> 00:24:56,660
+media بصوبها ممكن يكون في قبلات لكن في ال semi
+
+330
+00:24:56,660 --> 00:25:02,480
+-solid media بصوبها في تيوب بتركها تتصلب فتعطيني
+
+331
+00:25:02,480 --> 00:25:07,760
+اللي هي step هنتعرف عليها بالتفصيل في المعمل الجاي
+
+332
+00:25:07,760 --> 00:25:09,840
+هيك بتكون الشكل التيوب
+
+333
+00:25:16,030 --> 00:25:21,030
+التيوب بصب ال media فيه هلاقيه زي الجل متصلب هاي
+
+334
+00:25:21,030 --> 00:25:26,250
+ال .. هذا الاشي بسميه stabbing لأني هعمل طعن في ال
+
+335
+00:25:26,250 --> 00:25:31,670
+tube هنتعرف هلأ بالتفصيل كيف خطوات الشغل خطوات
+
+336
+00:25:31,670 --> 00:25:36,830
+الشغل بقولي انا بستخدم sterile needle اش يعني
+
+337
+00:25:36,830 --> 00:25:41,450
+sterile needle بالنسبة ل ال necrum loop ال necrum
+
+338
+00:25:41,450 --> 00:25:44,750
+loop بقولي احنا قولنا في عندي loop ال loop ممكن
+
+339
+00:25:44,750 --> 00:25:49,790
+تكونالانيكولاتينج لوب الانيكولاتينج لوب high فيه
+
+340
+00:25:49,790 --> 00:25:58,290
+بكون إلها حلقة لكن الانيكولاتينج needle ال loop ما
+
+341
+00:25:58,290 --> 00:26:03,890
+إلها حلقة يبقى ال loop high بسميها انيكولاتينج
+
+342
+00:26:03,890 --> 00:26:07,310
+loop فيه موجود فيها حلقة high ما بستخدمها في فحص
+
+343
+00:26:07,310 --> 00:26:12,170
+ال motility لكن الانيكولاتينج needleهي ال needle
+
+344
+00:26:12,170 --> 00:26:16,190
+بستخدمها في فحص ال motility باخد اللي هو ال colony
+
+345
+00:26:16,190 --> 00:26:22,670
+من الطبق وبعدين هزرعها في هايلة يوم طيب الخطوة
+
+346
+00:26:22,670 --> 00:26:25,830
+التانية يبقى عرفنا إيش يعني ال needle اللي هي loop
+
+347
+00:26:25,830 --> 00:26:29,510
+لكن مايلها حلقة بستخدمها في فحص اللي هو ال
+
+348
+00:26:29,510 --> 00:26:33,980
+motility أو أي فحص فيه اللي هو stabbingباخد اي
+
+349
+00:26:33,980 --> 00:26:37,740
+كولوني من الارجانيزم اللي انا ببحث هل هو motile او
+
+350
+00:26:37,740 --> 00:26:41,900
+non motile الخطوة التانية Stab quickly a tube of
+
+351
+00:26:41,900 --> 00:26:45,860
+semi-solid media بقولي اعملي Stabbing ايش يعني
+
+352
+00:26:45,860 --> 00:26:48,580
+Stabbing يعني اعملي طعنة في ال tube اللي انت
+
+353
+00:26:48,580 --> 00:26:51,800
+جهزتيها سيمي سوليد ميديا الميديا اللي بتحتوي على
+
+354
+00:26:51,800 --> 00:26:56,720
+أربع من عشرة بالمية أجاركيف بدي أعمل؟ بقولنا
+
+355
+00:26:56,720 --> 00:27:00,440
+بتجيبي الـ Nicolating Needle بعد ما أخدتي من
+
+356
+00:27:00,440 --> 00:27:05,080
+البكتيريا من الطبق اللي بدك تشوفه هل هو البكتيريا
+
+357
+00:27:05,080 --> 00:27:08,440
+motile أو non-motile بتدخلي الـ Nicolating Needle
+
+358
+00:27:08,440 --> 00:27:14,220
+و بتعملي طعنة تمام بسرعة بتعملي طعنة في التيوم
+
+359
+00:27:14,220 --> 00:27:18,560
+طبعا مش للآخر تقريبا بتوصل إلى التلتان و بتعملي
+
+360
+00:27:18,560 --> 00:27:23,690
+طعنة و بتطلعي بسرعةبعد ما اتخلصى بتعملى incubation
+
+361
+00:27:23,690 --> 00:27:28,050
+the semi solid media لمدة 24 ساعة في ال incubator
+
+362
+00:27:28,050 --> 00:27:31,690
+بدي اعمل incubation بحطه في ال incubator لمدة 24
+
+363
+00:27:31,690 --> 00:27:38,290
+ساعة بعد 24 ساعة بقرأ النتيجة قبل ما انا اعمل
+
+364
+00:27:38,290 --> 00:27:43,450
+stabbing لما افتحت ال tube طبعا لازم احنا من ال
+
+365
+00:27:43,450 --> 00:27:48,030
+aseptic technique اعقم فوهة ال tube وبعد ما اخلص
+
+366
+00:27:48,030 --> 00:27:52,690
+نفس الاشي اعقم فوهة ال tubeهذا من الـ Aseptic
+
+367
+00:27:52,690 --> 00:27:58,430
+technique بالنسبة للنتيجة النتيجة بتكون بعد 24
+
+368
+00:27:58,430 --> 00:28:02,530
+ساعة كيف بدي أفسر النتيجة بقولي pattern of growth
+
+369
+00:28:02,530 --> 00:28:06,550
+of motile organism the intermediate is turbid with
+
+370
+00:28:06,550 --> 00:28:09,730
+the growth of organism which has moved away from
+
+371
+00:28:09,730 --> 00:28:13,870
+the stop line بقولي هان لو كانت البكتيريا motile
+
+372
+00:28:13,870 --> 00:28:19,330
+كيف بدي أعرف بقولي أنه ان نمو البكتيريا بكونبتتحرك
+
+373
+00:28:19,330 --> 00:28:23,870
+على خط الزراع و كمان بلاحظ ان في انها تحركت كمان
+
+374
+00:28:23,870 --> 00:28:28,170
+بعيدا عن خط الزراع يبقى لو كانت motile ال media
+
+375
+00:28:28,170 --> 00:28:31,270
+هتكون اكيد turbid لأنها هتنمو البكتيريا سواء كانت
+
+376
+00:28:31,270 --> 00:28:35,150
+motile او non motile هيكون في عندي turbidity لكن
+
+377
+00:28:35,150 --> 00:28:40,070
+كيف بدي احدد هل هي motile او non motile بقوللي انه
+
+378
+00:28:40,070 --> 00:28:44,350
+في ال motile بتتحرك بعيدا عن خط الزراع لكن في ال
+
+379
+00:28:44,350 --> 00:28:48,300
+non motileفقط بلاقي الـ turbidity على خط الزراعة
+
+380
+00:28:48,300 --> 00:28:52,200
+لأنه ماقدرت تتحرك بعيدا فقط دلت على خط الزراعة
+
+381
+00:28:52,200 --> 00:28:57,300
+لأنه نمت بكتيريا pattern هيو has moved away from
+
+382
+00:28:57,300 --> 00:29:00,820
+the step line الموتالي بتتحرك بعيدا عن خط الزراعة
+
+383
+00:29:00,820 --> 00:29:03,920
+ال step line يعني خط الزراعة pattern of growth of
+
+384
+00:29:03,920 --> 00:29:08,860
+a non-motile organism only the step line is turbid
+
+385
+00:29:08,860 --> 00:29:12,640
+with growth هلاقي فقط انه مو ال turbidity فقط على
+
+386
+00:29:12,640 --> 00:29:17,130
+خط الزراعةبعض الأحيان ممكن انا ما اشوف هاي الـ
+
+387
+00:29:17,130 --> 00:29:21,910
+turbidity فممكن اضيف color indicator indicator كي
+
+388
+00:29:21,910 --> 00:29:24,730
+اشوف على ال media اضيفه قبل و انا بحضر ال media
+
+389
+00:29:24,730 --> 00:29:29,250
+ممكن اضيف indicator هذا ال indicator هيتلون ال
+
+390
+00:29:29,250 --> 00:29:33,130
+turbidity تبع البكتيريا بلون ال indicator وهك انا
+
+391
+00:29:33,130 --> 00:29:35,870
+بقدر اشوف ال turbidity بشكل واضح
+
+392
+00:29:38,670 --> 00:29:41,930
+يبقى الـ Motile و الـ Non-motile هيكون فيه تربط
+
+393
+00:29:41,930 --> 00:29:46,610
+لكن الفرق أن الـ Motility هتلاقي التربط بعيدا عن
+
+394
+00:29:46,610 --> 00:29:50,670
+خط الزراعة لكن الـ Non-motile ما بتقدر تتحرك فقط
+
+395
+00:29:50,670 --> 00:29:52,690
+هلاقي على خط الزراعة
+
+396
+00:30:01,190 --> 00:30:06,690
+هنقل خطوات بقوللي طبعا اول اشي بعقم بفتح الفوهة
+
+397
+00:30:06,690 --> 00:30:12,790
+للتيوب بعقم الفوهة بكون قابلها ماخدة طبعا اللي هي
+
+398
+00:30:12,790 --> 00:30:19,350
+big colony of bacteria ببيه ال needle بال needle
+
+399
+00:30:19,350 --> 00:30:23,830
+زي ما احنا شايفين مافيه لها حلقة بدخل بسرعة زي ما
+
+400
+00:30:23,830 --> 00:30:29,290
+احنا ملاحظين في المنتصف فقط لاتلتان بعدين باطلع
+
+401
+00:30:29,810 --> 00:30:35,190
+بعقم طبعا اللى هو ال needle و بعقم الفوهة و بحطها
+
+402
+00:30:35,190 --> 00:30:43,710
+في ال incubator هذه النتيجة هلأ بنشوف هذه بالنسبة
+
+403
+00:30:43,710 --> 00:30:48,330
+ل semisolid media with tetrazolium chloride اللى
+
+404
+00:30:48,330 --> 00:30:50,990
+هو ال color indicator احنا قلنا ممكن عشان اقدر ان
+
+405
+00:30:50,990 --> 00:30:55,800
+انا اشوف بشكل واضح ممكن استخدم indicatorتمام اضيفه
+
+406
+00:30:55,800 --> 00:30:59,760
+على ال media عشان اشوف انمو العكارة بشكل واضح هشوف
+
+407
+00:30:59,760 --> 00:31:03,560
+العكارة باللون الأحمر يبقى Tetrazolium chloride
+
+408
+00:31:03,560 --> 00:31:07,660
+يعتبر color indicator هذا مصطلح مهم ملاحظ في
+
+409
+00:31:07,660 --> 00:31:12,580
+الصورة انه انا هان اتلونت كل ال tube باللون الأحمر
+
+410
+00:31:12,580 --> 00:31:16,240
+يبقى العكارة موجودة على خط الزراع وعلى اتحركت
+
+411
+00:31:16,240 --> 00:31:20,660
+بعيدا عن خط الزراع يبقى هي positive لكن هان فقط
+
+412
+00:31:20,870 --> 00:31:24,630
+اللي العكارة فقط كانت على خط الزراعة اللي اتلون
+
+413
+00:31:24,630 --> 00:31:28,410
+باللون الأحمر خط الزراعة يبقى non motile هنبقى
+
+414
+00:31:28,410 --> 00:31:31,710
+نلاحظ انه اتحركت ايضا بعيد عن خط الزراعة يبقى هي
+
+415
+00:31:31,710 --> 00:31:36,290
+positive هك بنكون خلصنا ال bacterial motility
+
+416
+00:31:36,290 --> 00:31:38,910
+السلام عليكم ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/9AIy0KlGCSw_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/9AIy0KlGCSw_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..c826fa45f73dd222dceb5a85e38e99b7a7a33704
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/9AIy0KlGCSw_raw.srt
@@ -0,0 +1,1664 @@
+1
+00:00:00,720 --> 00:00:04,220
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:04,220 --> 00:00:07,780
+جميعا في هذا اللقاء هنتكلم عن ال bacterial
+
+3
+00:00:07,780 --> 00:00:12,700
+motility حركة البكتيريا البكتيريا بتتحرك بواسطة
+
+4
+00:00:12,700 --> 00:00:16,060
+الفلاجلة وعرفنا أن الفلاجلة يعتبر special
+
+5
+00:00:16,060 --> 00:00:19,360
+component يعني في بكتيريا عندي motile وفي بكتيريا
+
+6
+00:00:19,360 --> 00:00:24,120
+non motile عشان أعرف هل البكتيريا متحركة أو لأ في
+
+7
+00:00:24,120 --> 00:00:28,940
+عندي تلت طرق هنتعرف عليها في هذه المحاضرةأحد هذه
+
+8
+00:00:28,940 --> 00:00:33,300
+الطرق هي الـ Flagella Staining اللي يعتبر Special
+
+9
+00:00:33,300 --> 00:00:38,540
+Stain لو بدنا نعرف الـ Motility Motility The
+
+10
+00:00:38,540 --> 00:00:42,780
+ability of an organism to move by itself هو قدرة
+
+11
+00:00:42,780 --> 00:00:47,040
+ال micro organism أو البكتيريا على الحركة بنفسها
+
+12
+00:00:47,040 --> 00:00:52,640
+by itself هذا هو تعريف ال Motility قدرة ال micro
+
+13
+00:00:52,640 --> 00:00:55,660
+organism على الحركة بنفسها by itself
+
+14
+00:01:00,530 --> 00:01:05,030
+ليش by itself لأنه ممكن تتحرك by activity of water
+
+15
+00:01:05,030 --> 00:01:09,070
+molecule بواسطة حركة جزيئات الماء وهي تعتبر
+
+16
+00:01:09,070 --> 00:01:13,290
+Brownian movement لكن لو اتحركت البكتيريا by
+
+17
+00:01:13,290 --> 00:01:18,170
+itself بسميها ل true movement motility is closely
+
+18
+00:01:18,170 --> 00:01:21,810
+linked with chemotaxis مصطلح ال motility مرتبط
+
+19
+00:01:21,810 --> 00:01:26,190
+بمصطلح تاني اسمه ال chemotaxis لو بدنا نعرف ال
+
+20
+00:01:26,190 --> 00:01:30,310
+chemotaxisThe ability to oriented along certain
+
+21
+00:01:30,310 --> 00:01:34,910
+chemical gradient هي حركة البكتيريا حسب تركيز
+
+22
+00:01:34,910 --> 00:01:38,850
+المواد الكيميائية لو كان في عندي مغذيات أكيد
+
+23
+00:01:38,850 --> 00:01:43,150
+هتتحرك البكتيريا باتجاه هذه المغذيات لو كان في
+
+24
+00:01:43,150 --> 00:01:47,990
+عندي toxic هتتحرك بعيد عن ال toxic هتهرب منهاA
+
+25
+00:01:47,990 --> 00:01:51,590
+large number of bacteria are motile، أعداد كبيرة
+
+26
+00:01:51,590 --> 00:01:56,070
+من البكتيريا تعتبر motile، يبقى النسبة القليلة
+
+27
+00:01:56,070 --> 00:02:00,330
+يعتبر non-motile Most bosses one or more flagella
+
+28
+00:02:00,330 --> 00:02:04,310
+on their surface that allow them to swim البكتيريا
+
+29
+00:02:04,310 --> 00:02:08,790
+المتحركة بتتحرك بالفلاجلة بالأصوات، بتمتلك إما صوت
+
+30
+00:02:08,790 --> 00:02:13,630
+واحد أو مجموعة أصوات على السطح بيسمح إلها بالحركة
+
+31
+00:02:14,290 --> 00:02:17,990
+بكتيريا الفلاجلة لو بدنا نعرف الفلاجلة are tiny
+
+32
+00:02:17,990 --> 00:02:22,810
+hell-like organelles of locomotion هي عبارة عن
+
+33
+00:02:22,810 --> 00:02:28,970
+زواق دقيقة زي الشعر تستخدم في الحركة من وين بينشأ
+
+34
+00:02:28,970 --> 00:02:33,190
+الفلاجلة originating in the cytoplasm بينشأ من
+
+35
+00:02:33,190 --> 00:02:38,930
+الcytoplasm beneath the cell wall بيطلع الصوت خارج
+
+36
+00:02:38,930 --> 00:02:43,830
+ال cell wall they extend beyond the cellويمتد خارج
+
+37
+00:02:43,830 --> 00:02:48,910
+الخلية بقولي طول الصوت ممكن يكون بيساوي طول الخلية
+
+38
+00:02:48,910 --> 00:02:53,890
+البكتيرية او اطول منها بشوية their fine protein
+
+39
+00:02:53,890 --> 00:02:57,850
+structure عن اي protein بيقصد في هاي ال slide
+
+40
+00:02:57,850 --> 00:03:05,090
+بقولي ان الفلاجلة بتكون من protein بسميه الفلاجلين
+
+41
+00:03:05,090 --> 00:03:09,370
+هذا ال protein يعتبر fine خفيف مابقدر اشوفه
+
+42
+00:03:09,370 --> 00:03:13,250
+بالتالي محتاجة requireSpecialist Training
+
+43
+00:03:13,250 --> 00:03:17,710
+Technique محتاجة لصبغات خاصة for demonstrating
+
+44
+00:03:17,710 --> 00:03:21,410
+them with a light microscope عشان أقدر أشوفه تحت
+
+45
+00:03:21,410 --> 00:03:24,690
+ال microscope يبقى ال protein المقصود في هذه
+
+46
+00:03:24,690 --> 00:03:29,370
+الجملة الفلاجلين لأنه الفلاجلة بتتكون من ال
+
+47
+00:03:29,370 --> 00:03:31,150
+protein اسمه الفلاجلين
+
+48
+00:03:36,770 --> 00:03:41,210
+site of a flagella موقع الأصوات the pattern of
+
+49
+00:03:41,210 --> 00:03:44,370
+flagellation is an important feature in
+
+50
+00:03:44,370 --> 00:03:48,550
+identification of motile bacteria ولتوزيع الأصوات
+
+51
+00:03:48,550 --> 00:03:52,890
+للبكتيريا المتحركة مهم جدا في ال identification زي
+
+52
+00:03:52,890 --> 00:03:55,790
+ما قلنا في ال Indus Bar location أنه بيساعد في
+
+53
+00:03:55,790 --> 00:03:58,950
+تعريف البكتيريا كمان ال site of a flagella بيساعد
+
+54
+00:03:58,950 --> 00:04:03,370
+في ال identification لو كانت البكتيريا is a single
+
+55
+00:04:03,370 --> 00:04:08,280
+polar flagelliumبسمي البكتيريا mono-tracheas لو
+
+56
+00:04:08,280 --> 00:04:13,260
+كانت بتمتلك صوت واحد فقط أكيد هيكون على قطب واحد
+
+57
+00:04:13,260 --> 00:04:21,200
+بسمي البكتيريا mono-tracheas amphitracheas one or
+
+58
+00:04:21,200 --> 00:04:25,440
+more في الجلة at both poles لو كان في عندي صوت
+
+59
+00:04:25,440 --> 00:04:32,600
+واحد لكن على كلا القطبينصوت واحد على كيلة القطبين
+
+60
+00:04:32,600 --> 00:04:36,640
+بسميه Amphitrachias او مجموعة أصوات ممكن يكون
+
+61
+00:04:36,640 --> 00:04:41,340
+مجموعة أصوات على الكيلة القطبين بسميه
+
+62
+00:04:41,340 --> 00:04:45,660
+Amphitrachias law for trachias tufts of flagella
+
+63
+00:04:45,660 --> 00:04:49,440
+at one end pole لو كان في عندي حزمة tufts يعني
+
+64
+00:04:49,440 --> 00:04:54,040
+حزمة of flagella at one end pole لو كان في عندي
+
+65
+00:04:54,040 --> 00:04:59,180
+حزمة من الأصوات موجودة على قطب واحد فقط بسميها law
+
+66
+00:04:59,180 --> 00:05:03,090
+for trachiasزي هك فقط على قطب واحد مش زي الـ
+
+67
+00:05:03,090 --> 00:05:07,330
+Amphitracius على القطبين الـ Monotracius صوت على
+
+68
+00:05:07,330 --> 00:05:12,350
+قطب لكن الـ Lymphotracius مجموعة أصوات على قطب
+
+69
+00:05:12,350 --> 00:05:17,050
+واحد Peritracius لو كانت الأصوات تحيط في الخلية
+
+70
+00:05:17,050 --> 00:05:21,190
+البكتيرية زي ما أحنا شايفين بالصورة هذه الأصوات
+
+71
+00:05:21,190 --> 00:05:26,770
+بتحيط في الخلية البكتيرية بسميها Peritracius طيب
+
+72
+00:05:26,770 --> 00:05:30,780
+الـ Tracius يعني نفات الصفةnon-motile يعني ما
+
+73
+00:05:30,780 --> 00:05:34,460
+بتتحرك without إفلاجلة أكيد مش هيكون عندها إفلاجلة
+
+74
+00:05:34,460 --> 00:05:38,800
+بتكون attracted ما عندها أي إفلاجلة هذا بالنسبة لل
+
+75
+00:05:38,800 --> 00:05:42,820
+site of إفلاجلة قولنا إن ال site of إفلاجلة بيساعد
+
+76
+00:05:42,820 --> 00:05:46,320
+في ال identification كيف لو كان عندي أنا عملت
+
+77
+00:05:46,320 --> 00:05:50,200
+إجرام أسنان و طلعت النتيجة إجرام negative Bacilli
+
+78
+00:05:50,200 --> 00:05:53,700
+أكيد الإجرام negative Bacilli بتضم مجموعة كبيرة من
+
+79
+00:05:53,700 --> 00:05:57,740
+البكتيريا عملت مجموعة فحوصات بالنهاية وصلت إنه
+
+80
+00:05:58,000 --> 00:06:03,680
+البكتيريا إما X أو Y كانت XY motile لكن توزيع
+
+81
+00:06:03,680 --> 00:06:07,640
+الأصوات تبعهم مختلف من خلال الـ site of the
+
+82
+00:06:07,640 --> 00:06:17,440
+flagella بقدر أعمل identification most
+
+83
+00:06:17,440 --> 00:06:21,640
+motile bacteria move in a straight line for a
+
+84
+00:06:21,640 --> 00:06:25,240
+brief time بقولي معظم البكتيريا المتحركة بتتحرك
+
+85
+00:06:25,240 --> 00:06:30,290
+بخط مستقيم لفترةو جيزة then turn and randomly
+
+86
+00:06:30,290 --> 00:06:33,870
+change direction before swimming again بقولي بعدها
+
+87
+00:06:33,870 --> 00:06:38,470
+بتغير الاتجاه و بتتحرك بشكل عشوائي ال flagella
+
+88
+00:06:38,470 --> 00:06:42,530
+composed from بقولي من أش بتكون ال flagella بتكون
+
+89
+00:06:42,530 --> 00:06:46,970
+من filament composed of a protein called flagellin
+
+90
+00:06:46,970 --> 00:06:52,380
+قلنا على ال flagellinبتكون الفلاجلة من filament
+
+91
+00:06:52,380 --> 00:06:56,400
+هذا ال filament بتكون من ال protein الفلاجلين يبقى
+
+92
+00:06:56,400 --> 00:07:01,880
+الفلاجلين a protein that composed filament ال hook
+
+93
+00:07:01,880 --> 00:07:05,400
+الخطاف base of filament near the cell wall هو
+
+94
+00:07:05,400 --> 00:07:10,360
+قاعدة ال filament تكون قريب من ال cell wallبازل
+
+95
+00:07:10,360 --> 00:07:14,240
+باضى آخر مكون البازل باضى بيقول لي بيربط انكار
+
+96
+00:07:14,240 --> 00:07:18,040
+بيربط او بيدعم ال filament و ال hook to the cell
+
+97
+00:07:18,040 --> 00:07:22,600
+wall بيربط و بيدعم ال filament و ال hook بال cell
+
+98
+00:07:22,600 --> 00:07:28,880
+wall فى عندي رسمة هى ال filament اللى قولنا بتكون
+
+99
+00:07:28,880 --> 00:07:32,580
+من بروتين لفلاجلين فى عندي ال hook الخطافة هو
+
+100
+00:07:32,580 --> 00:07:36,920
+قاعدة ال filament و فى عندي البازل باضى اللى بيربط
+
+101
+00:07:36,920 --> 00:07:42,460
+ال hook و ال filament بال cell walltype of
+
+102
+00:07:42,460 --> 00:07:47,840
+movement ممكن تكون عندي run run straight line
+
+103
+00:07:47,840 --> 00:07:50,780
+movement occur when the flagella rotate counter
+
+104
+00:07:50,780 --> 00:07:54,720
+clockwise البكتيريا بتتحرك بنوعين من الحركة إما
+
+105
+00:07:54,720 --> 00:08:00,740
+run تتحرك عكس عقارب الساعة tumble اللي هي turning
+
+106
+00:08:00,740 --> 00:08:03,960
+the direction by clockwise movement of the
+
+107
+00:08:03,960 --> 00:08:09,530
+flagella بتكون حركة عشوائية و مع عقارب الساعةهذا
+
+108
+00:08:09,530 --> 00:08:15,050
+بالنسبة للـ type of movement هنا
+
+109
+00:08:15,050 --> 00:08:20,370
+في السلايدات ذا كل ال site of flagella قلنا لو
+
+110
+00:08:20,370 --> 00:08:24,910
+كانت بتحيط بالخلية البكتيرية بسمي البكتيريا
+
+111
+00:08:24,910 --> 00:08:30,470
+periatriace من الأمثلة عليها ال E.coli نحفظ المثال
+
+112
+00:08:30,470 --> 00:08:34,690
+E.coli periatriace الفلاجلة بتحيط بالخلية
+
+113
+00:08:34,690 --> 00:08:41,230
+البكتيريةصوت واحد على قطب واحد mono-tracheas مثال
+
+114
+00:08:41,230 --> 00:08:47,530
+عليها الـ Vibrio cholera هنا عندي أنا حزمة من
+
+115
+00:08:47,530 --> 00:08:52,850
+الأصوات على صوت واحد بسميها الـ Lo-Fo Tracheas ذا
+
+116
+00:08:52,850 --> 00:08:58,030
+كل مثال الـ Sodomonas نحذف هذا ال Sodomonas هي
+
+117
+00:08:58,030 --> 00:09:03,550
+تعتبر mono-tracheas وليس Lo-Fo Tracheasيبقى نعدل
+
+118
+00:09:03,550 --> 00:09:06,610
+على ال slide أن الـ Sodomonas لا تعتبر Loft
+
+119
+00:09:06,610 --> 00:09:11,070
+Tracheas تعتبر Monotracheas يبقى Loft Tracheas لما
+
+120
+00:09:11,070 --> 00:09:15,270
+يكون في عندي حزمة من الأصوات على قطب واحد لما يكون
+
+121
+00:09:15,270 --> 00:09:19,470
+في عندي one or more flagella at both poles لما
+
+122
+00:09:19,470 --> 00:09:22,330
+يكون في عندي صوت واحد أو مجموعة أصوات على كلا
+
+123
+00:09:22,330 --> 00:09:26,410
+القطبين بسميها Amphitracheas مثال عليها الـ
+
+124
+00:09:26,410 --> 00:09:30,230
+Spirulum والتانستمام يبقى هدول كل الأمثلة اللي
+
+125
+00:09:30,230 --> 00:09:34,270
+ذكرناها يعتبروا motile لكن ال site of flagella
+
+126
+00:09:34,270 --> 00:09:43,750
+بيختلف motility
+
+127
+00:09:43,750 --> 00:09:49,430
+testing motility called by بيقوللي ممكن أنا أكشف
+
+128
+00:09:49,430 --> 00:09:53,310
+عن ال motility عن البكتيريا ال motility في تلت طرق
+
+129
+00:09:53,310 --> 00:09:58,340
+عشان أعرف هل البكتيريا motile or non motileالطريقة
+
+130
+00:09:58,340 --> 00:10:02,460
+الأولى الـ Hanging Drop Technique طريقة تانية
+
+131
+00:10:02,460 --> 00:10:06,280
+لفلاجلة استان اللى يعتبر special استان الطريقة
+
+132
+00:10:06,280 --> 00:10:10,040
+التالتة و اللى هى most common semisolid media
+
+133
+00:10:10,040 --> 00:10:15,580
+inculation أول طريقة اللى هى ال hanging drop slide
+
+134
+00:10:15,580 --> 00:10:20,860
+hanging معناها معلقة هى تعتبر الطريقة زى ال wet
+
+135
+00:10:20,860 --> 00:10:24,140
+mouth اللى قلنا من خلالها بقدر اتعرف على ال
+
+136
+00:10:24,140 --> 00:10:28,450
+bacterial morphology قلناالـ wet mouth بقدر اشوف
+
+137
+00:10:28,450 --> 00:10:33,770
+living bacteria living حية يعني بقدر اشوفها
+
+138
+00:10:33,770 --> 00:10:38,350
+ابتتحرك يعني هشوف حركة البكتيريا لكن انا لو صبغت
+
+139
+00:10:38,350 --> 00:10:42,070
+مش هشوف ال motility مش هشوف البكتيريا ابتتحرك لأن
+
+140
+00:10:42,070 --> 00:10:44,670
+احنا قولنا من خطوات الصباغة في ال heat في اندي
+
+141
+00:10:44,670 --> 00:10:50,650
+heat fixation بيعمل كلب يوقف ال movementفي الـ wet
+
+142
+00:10:50,650 --> 00:10:53,370
+mouth العادية اللي شرحناها قولنا إنه إحنا بنجيب
+
+143
+00:10:53,370 --> 00:10:57,990
+slide بنحط drop of bacterial suspension ومنحط فوق
+
+144
+00:10:57,990 --> 00:11:02,030
+cover slip هذه الطريقة ال wet mouth العادية اللي
+
+145
+00:11:02,030 --> 00:11:06,550
+شرحناها بيقولنا إنها بتشكل ضغط على ال slide فبتعيق
+
+146
+00:11:06,550 --> 00:11:10,090
+حركة البكتيريا فممكن البكتيريا تكون multi لكن
+
+147
+00:11:10,090 --> 00:11:16,860
+الضغط اللي شكله cover slip على ال slide هيعيق حركة
+
+148
+00:11:16,860 --> 00:11:21,840
+البكتيريا فبالتالي ممكن اشوفها non motile غير انه
+
+149
+00:11:21,840 --> 00:11:26,460
+في هذه الطريقة مش هقدر اميز ل true movement حركة
+
+150
+00:11:26,460 --> 00:11:31,780
+البكتيريا by itself by فلاجلة عن حركة البكتيريا ال
+
+151
+00:11:31,780 --> 00:11:36,280
+brownian movement اللي هي حركة جزيئات السائل يبقى
+
+152
+00:11:36,280 --> 00:11:39,600
+في ال wet mouth العادية لما اجيب slide و احط
+
+153
+00:11:39,600 --> 00:11:44,030
+bacterial suspension و cover slipهيضحيق حركة
+
+154
+00:11:44,030 --> 00:11:48,810
+البكتيريا لأنها تشكل ضغط تاني إشي مش هقدر أميز ال
+
+155
+00:11:48,810 --> 00:11:52,790
+true movement عن ال brownian movement عشان هيك
+
+156
+00:11:52,790 --> 00:11:56,830
+عملنا modification لنا ها لهذه الطريقة عشان أقدر
+
+157
+00:11:56,830 --> 00:12:01,350
+أحدد هل البكتيريا motile بتتحرك by itself أو non
+
+158
+00:12:01,350 --> 00:12:06,630
+motile الطريقة اسمها ال hanging drop slide بستخدم
+
+159
+00:12:06,630 --> 00:12:11,330
+slide بتتعلق فيها البكتيريا اسمها depression slide
+
+160
+00:12:12,340 --> 00:12:15,740
+ماهي الـ depression slide بقول هي slide that has a
+
+161
+00:12:15,740 --> 00:12:19,820
+concavity يعني إلها تقعر زي ما ملاحظين هي ال
+
+162
+00:12:19,820 --> 00:12:25,100
+depression slide في إلها بيكون فيه تقعر هاي ال
+
+163
+00:12:25,100 --> 00:12:30,860
+slide نكمل هي slide that has a concavity and cover
+
+164
+00:12:30,860 --> 00:12:34,820
+slip can be placed on it يعني ممكن كمان أني أنا
+
+165
+00:12:34,820 --> 00:12:38,740
+أحط ال cover slip فوق عليها يبقى هاي هي ال
+
+166
+00:12:38,740 --> 00:12:42,890
+depression slideبجيب drop of bacteria و بحطها على
+
+167
+00:12:42,890 --> 00:12:46,310
+cover slab وبعدها بقلبها و بحطها فوق depression
+
+168
+00:12:46,310 --> 00:12:51,150
+slide هيك بتتعلق البكتيريا طيب اتعلقت طبعا
+
+169
+00:12:51,150 --> 00:12:55,590
+البكتيريا على السطح هيك بقدر بكل سهولة احدد حركة
+
+170
+00:12:55,590 --> 00:12:59,750
+البكتيريا طيب لو كان ماعندي انا depression slide و
+
+171
+00:12:59,750 --> 00:13:03,070
+بدي اشوف البكتيريا ال mutilative طبعا هي slide
+
+172
+00:13:03,070 --> 00:13:06,970
+خاصة اسمها depression slide بكون فيه تقاعرطب انا
+
+173
+00:13:06,970 --> 00:13:10,010
+ماعندي هاي ال slide ممكن اقدر احدد ال bacterial
+
+174
+00:13:10,010 --> 00:13:13,870
+motility اقدر اعمل اشي اعمل زي ال depression slide
+
+175
+00:13:13,870 --> 00:13:19,270
+بقولنا اه بتجيب slide عادية تمام بتجيب slide عادية
+
+176
+00:13:19,270 --> 00:13:24,150
+و بجيب ال parafilm ال parafilm هو ورق بمغطب
+
+177
+00:13:24,150 --> 00:13:27,930
+نستخدمه أحيانا فيه عشان نسكر ال tube هذا ال
+
+178
+00:13:27,930 --> 00:13:32,950
+parafilm بروح انا بلف ال parafilm بجدل ال parafilm
+
+179
+00:13:32,950 --> 00:13:39,310
+و بعمل دائرة زي دائرةطبعا هي هتلذق على ال slide
+
+180
+00:13:39,310 --> 00:13:43,190
+باجي على نص ال slide هيك بيصير انا عندي concave
+
+181
+00:13:43,190 --> 00:13:49,450
+والسطح مقعر بجيب ال cover slip cover slip عادي بحط
+
+182
+00:13:49,450 --> 00:13:52,790
+drop of بكتيريا و بقلبها و بحطها فوق ال concave
+
+183
+00:13:52,790 --> 00:13:56,610
+والhigh اللي هي ال barifilm اللي أنا لفتها هيك انا
+
+184
+00:13:56,610 --> 00:14:01,190
+حصلت على depression slide صارت البكتيريا معلقة ما
+
+185
+00:14:01,190 --> 00:14:05,700
+بين ال cover slip و ما بين ال slideيبقى نقرا the
+
+186
+00:14:05,700 --> 00:14:09,820
+slide depression slide for hanging drop تستخدم ال
+
+187
+00:14:09,820 --> 00:14:13,940
+depression slide for hanging drop is a ground with
+
+188
+00:14:13,940 --> 00:14:19,260
+a concave well بقوللي فيها حفرة او فتحة مقعرة in
+
+189
+00:14:19,260 --> 00:14:21,440
+the center زي ما احنا شايفين ال depression slide
+
+190
+00:14:21,440 --> 00:14:25,800
+في عيني انا فتحة concave well فتحة مقعرة في
+
+191
+00:14:25,800 --> 00:14:30,280
+المنتصف the caverous glass hold a drop of the
+
+192
+00:14:30,280 --> 00:14:34,100
+suspension قولنا ال cover glass او ال cover slip
+
+193
+00:14:34,440 --> 00:14:37,520
+منحط عليه اللي هو ال bacterial suspension اللي هو
+
+194
+00:14:37,520 --> 00:14:42,840
+drop of bacteria when the gaffer when the gaffer
+
+195
+00:14:42,840 --> 00:14:47,160
+class is inverted over the well of the slide بقولي
+
+196
+00:14:47,160 --> 00:14:51,140
+لما أنا ال gaffer slip ها ده لبع على ال well هاي
+
+197
+00:14:51,140 --> 00:14:55,200
+اللي موجودة على ال slide the drop hangs from the
+
+198
+00:14:55,200 --> 00:15:00,060
+class in the hollow concavity of the slide بيصير
+
+199
+00:15:00,060 --> 00:15:03,650
+ال drop ofبكتيريا اللي كانت على ال cover slip
+
+200
+00:15:03,650 --> 00:15:08,350
+معلقة في ال concave well since the drop lies
+
+201
+00:15:08,350 --> 00:15:14,010
+within an enclosed glass chamber بقولنا عشان انا
+
+202
+00:15:14,010 --> 00:15:19,810
+احفظ ممكن احنا نحط اللي هو ال vaseline ال vaseline
+
+203
+00:15:19,810 --> 00:15:24,290
+تمام هذا ال vaseline ممكن انا احطه على حوالين ال
+
+204
+00:15:24,290 --> 00:15:31,350
+cover slip عشان يمنع الجفاف from drying outعشان
+
+205
+00:15:31,350 --> 00:15:38,150
+يمنع ممكن
+
+206
+00:15:38,150 --> 00:15:42,250
+نحط Vaseline حوالين اللي هو around the edge of the
+
+207
+00:15:42,250 --> 00:15:46,150
+cover slip حوالين ال cover slip هيحفظ ليها من
+
+208
+00:15:46,150 --> 00:15:49,730
+الجفاف ليش لأنه لو جفت البكتيريا مش هتقدر تتحرك
+
+209
+00:15:49,730 --> 00:15:53,830
+هتموت مش هتتحرك مش هقدر احدد هل البكتيريا motile
+
+210
+00:15:53,830 --> 00:15:57,290
+او non motile يبقى بعد ما احط ال cover slip علىالـ
+
+211
+00:15:57,290 --> 00:16:00,330
+depression slide أو على اللي انا حطيته هنا الـ
+
+212
+00:16:00,330 --> 00:16:04,850
+parafilm بحط Vaseline حوالين الـ cover slip هيك
+
+213
+00:16:04,850 --> 00:16:11,970
+هيحميلي البكتيريا من الجفا هذا بالنسبة للـ hanging
+
+214
+00:16:11,970 --> 00:16:15,390
+drop slide طبعا انا البكتيريا الـ bacteria
+
+215
+00:16:15,390 --> 00:16:23,260
+motility هتشوفها في hand على ال edge علىالـ edge
+
+216
+00:16:23,260 --> 00:16:27,120
+من اللي هو الـ concave well أو اللي هو اللي حطيته
+
+217
+00:16:27,120 --> 00:16:32,180
+البارع فيلم بقول
+
+218
+00:16:32,180 --> 00:16:35,700
+لي notes ملاحظة you should to be able to
+
+219
+00:16:35,700 --> 00:16:40,160
+differentiate true movement from Brownian movement
+
+220
+00:16:40,160 --> 00:16:43,240
+or motility يبقى احنا قلنا انه في عندي فرق بين ال
+
+221
+00:16:43,240 --> 00:16:46,840
+true movement و ال brownian movement ال brownian
+
+222
+00:16:46,840 --> 00:16:50,200
+movement بقول لي هي حركة جزيئات السائل activity of
+
+223
+00:16:50,200 --> 00:16:54,370
+water moleculeلكن بالنسبة لـ Retromovement اللي هي
+
+224
+00:16:54,370 --> 00:16:59,130
+حركة البكتيريا نفسها runs and tumble اللي هي تعتبر
+
+225
+00:16:59,130 --> 00:17:09,310
+حركة البكتيريا by itself by فلاجلة قلنا
+
+226
+00:17:09,310 --> 00:17:13,510
+عندي تلت طرق عشان أعرف هل البكتيريا motile أو non
+
+227
+00:17:13,510 --> 00:17:16,430
+-motile الطريقة الأولى اللي هي hanging drop
+
+228
+00:17:16,430 --> 00:17:20,280
+technique الطريقة التانية لفلاجلة استينةطريقة
+
+229
+00:17:20,280 --> 00:17:23,140
+تانية عشان أعرف هل البكتيريا non-motile أو non
+
+230
+00:17:23,140 --> 00:17:28,220
+-motile بدي أصبغ الفلاجلة المسؤول عن الحركة
+
+231
+00:17:28,220 --> 00:17:34,100
+الفلاجلة قولنا هو تنيق ضعيف خفيف لازم أنا أصبغه
+
+232
+00:17:34,100 --> 00:17:37,740
+عشان أقدر أشوفه flagella should be amplified
+
+233
+00:17:37,740 --> 00:17:41,400
+enlarged لازم أني أنا أكبره use a standard that is
+
+234
+00:17:41,400 --> 00:17:47,810
+especially deposit on flagellaincreasing diameter
+
+235
+00:17:47,810 --> 00:17:52,230
+بقول لي انا لما استخدم لستين هي الصبغة هتترسب على
+
+236
+00:17:52,230 --> 00:17:55,650
+ال flagella بالتالي هيزيد قطر ال flagella و اقدر
+
+237
+00:17:55,650 --> 00:18:00,730
+اني انا اشوفه some
+
+238
+00:18:00,730 --> 00:18:05,170
+في اندي انا طريقتين للصباغة قبل ما نحكي على
+
+239
+00:18:05,170 --> 00:18:09,250
+الطريقتين كأي خطوة من خطوات الصباغة هعمل smearing
+
+240
+00:18:09,250 --> 00:18:13,490
+preparation air drying لكن ال fixation هعمل
+
+241
+00:18:13,490 --> 00:18:19,860
+formalin fixationformalin fixation هعمل مش هي
+
+242
+00:18:19,860 --> 00:18:25,060
+fixation تمام رابع خطوة لستان اللي قولنا بتختلف
+
+243
+00:18:25,060 --> 00:18:28,180
+حسب نوع الاستان بالنسبة لفلاجلة استان في عندي
+
+244
+00:18:28,180 --> 00:18:32,020
+طريقتين الطريقة الأولى بستخدم ال primary استان
+
+245
+00:18:32,020 --> 00:18:37,360
+rosanilin-di يبقى ال primary استان هي rosanilin-di
+
+246
+00:18:37,360 --> 00:18:39,000
+بقولي بستخدمها
+
+247
+00:18:46,070 --> 00:18:49,730
+الـ rose-aniline dye هي الـ primary stain وبستخدم
+
+248
+00:18:49,730 --> 00:18:55,030
+أي ماردانت تمام بنفس الطريقة عملت سميري ب
+
+249
+00:18:55,030 --> 00:18:59,990
+preparation اير دراينج فورمالين فكساشن بعدها غمرت
+
+250
+00:18:59,990 --> 00:19:04,370
+ال slide بالـ rose-aniline dye اللي هي ال primary
+
+251
+00:19:04,370 --> 00:19:09,290
+stain عملت washing حطيت ماردانت تمام بعد هيك أنا
+
+252
+00:19:09,290 --> 00:19:13,750
+بقدر احدد اللي هي اشوف تحت ال microscope اللي هو
+
+253
+00:19:13,750 --> 00:19:18,830
+ال flagella هشوفهطبعاً بشكل واضح وكانتني لكن أنا
+
+254
+00:19:18,830 --> 00:19:22,190
+بعد ما صبغت الصبغة هتترسب على الـ Flagella هقدر
+
+255
+00:19:22,190 --> 00:19:26,690
+اني انا اشوفه Apply to bacterial suspension fixed
+
+256
+00:19:26,690 --> 00:19:31,150
+in formalin طبعا احنا من حط الـ Rosanilin dye and
+
+257
+00:19:31,150 --> 00:19:35,650
+المردن لبكتيريا ال suspension اللي انا تبته مسبقا
+
+258
+00:19:35,650 --> 00:19:39,710
+بال Formalin and spread across a glass slide
+
+259
+00:19:39,710 --> 00:19:44,790
+بقوللي ال Formalin linked to or fixed the flagella
+
+260
+00:19:45,300 --> 00:19:48,260
+أيش وظيفة ال Formalin اللي هو بيعمل fixation
+
+261
+00:19:48,260 --> 00:19:52,820
+لفلاجلة and other surface proteins of the cell The
+
+262
+00:19:52,820 --> 00:19:56,120
+dye اللي هي ال rose aniline dye and mordant then
+
+263
+00:19:56,120 --> 00:20:01,160
+precipitate around this fixed surface بقولي الصبغة
+
+264
+00:20:01,160 --> 00:20:05,330
+و ال mordant هيترسبواحوالين اللي هو الـ Flagella
+
+265
+00:20:05,330 --> 00:20:10,750
+اللي تم تثبيته بالـ Formalin Enlarging their
+
+266
+00:20:10,750 --> 00:20:14,750
+diameter، هيزيد حجم اللي هو الـ Diameter تبع الـ
+
+267
+00:20:14,750 --> 00:20:18,050
+Flagella and making Flagella visible when viewed
+
+268
+00:20:18,050 --> 00:20:23,030
+under the microscope، هيك أنا هقدر أني أشوفه تحت
+
+269
+00:20:23,030 --> 00:20:27,250
+الـ Microscopeبالنسبة للصبغة الروز أنالين داي
+
+270
+00:20:27,250 --> 00:20:31,790
+بتعطي اللون الأحمر راح يترسب حول الفلاجلة اللى تم
+
+271
+00:20:31,790 --> 00:20:34,870
+تثبيته بالفرمالين بالتالي هيك هيزيد حجمه هذه
+
+272
+00:20:34,870 --> 00:20:39,350
+بالنسبة للطريقة الأولى الطريقة التانية في الصباغة
+
+273
+00:20:39,350 --> 00:20:43,430
+في الفلاجلة الستان بقوللي بستخدم ال silver nitrate
+
+274
+00:20:43,430 --> 00:20:47,290
+كprimer استان وفرقتنات موردان بستخدم موردان
+
+275
+00:20:47,290 --> 00:20:51,590
+تلفرقتنات و ال silver nitrate يبقى بعمل smear
+
+276
+00:20:51,590 --> 00:20:56,570
+preparation air dryingfor maline fixation الصبغة
+
+277
+00:20:56,570 --> 00:21:01,010
+سيلفر نيترات هتعطي لون أسود والموردانت فى
+
+278
+00:21:01,010 --> 00:21:05,130
+الريكدانات موردانت بقولى هذا الصبغة مع الموردانت
+
+279
+00:21:05,130 --> 00:21:09,090
+هيتم تعريضها للبكتيريا ال suspension النتيجة
+
+280
+00:21:09,090 --> 00:21:12,710
+هيتكون dark precipitates that form on the bacteria
+
+281
+00:21:12,710 --> 00:21:16,010
+and their and their flagella allow them to be
+
+282
+00:21:16,010 --> 00:21:20,020
+easilyهقدر اني انا اشوفها تحت الماكروسكوب بكل
+
+283
+00:21:20,020 --> 00:21:23,400
+سهولة الصبغة وال موردانت هيترسبوا على الفلاجلة
+
+284
+00:21:23,400 --> 00:21:27,160
+فبالتالي هيزيد ال diameter للفلاجلة هقدر اني انا
+
+285
+00:21:27,160 --> 00:21:31,220
+اشوفها Silver Nitrate قولنا بيعطي اللوان الأسود
+
+286
+00:21:31,220 --> 00:21:35,460
+Silver Blatting Technique هاي الطريقة بستخدمها
+
+287
+00:21:35,460 --> 00:21:39,860
+used to stain the very slender spirot sheet بقوللي
+
+288
+00:21:39,860 --> 00:21:43,260
+هاي الطريقة انا بستخدمها في بكتيريا لصباغة هذه
+
+289
+00:21:43,260 --> 00:21:48,880
+البكتيريا very slenderSpiroid Sheet بكتيريا ماخدة
+
+290
+00:21:48,880 --> 00:21:52,860
+شكل الـ Spiral هاي الطريقة اللي هي الـ Silver
+
+291
+00:21:52,860 --> 00:21:57,360
+Nitrate و الفرقتانات Mordant يبقى في هاي الطريقة
+
+292
+00:21:57,360 --> 00:22:00,840
+بستخدم الـ Silver Nitrate ك Primary Stain و
+
+293
+00:22:00,840 --> 00:22:04,880
+Mordant الفرقتانات هضيفهم للـ Bacterial Suspension
+
+294
+00:22:04,880 --> 00:22:10,440
+هيترسبوا حول الفلاجلة وبالتالي هيعطي اللون الأسود
+
+295
+00:22:10,440 --> 00:22:14,770
+بالتالي سهل ان انا اشوفهم تحت ال microscopeهذه
+
+296
+00:22:14,770 --> 00:22:19,390
+الطريقة التانية ملاحظين في الصورة الفلاجولة اقدرت
+
+297
+00:22:19,390 --> 00:22:23,650
+اني انا اشوفه هي مسبوخ بالروزانالين داي اللي بتعطي
+
+298
+00:22:23,650 --> 00:22:28,690
+اللون الزهري لكن بالنسبة لهذه الصورة المسبوخ بالـ
+
+299
+00:22:28,690 --> 00:22:33,070
+silver nitrate زائد الفيرركتانات هيعطي اللون
+
+300
+00:22:33,070 --> 00:22:38,230
+الأسود آخر طريقة في اللي هو ال bacterial motility
+
+301
+00:22:38,230 --> 00:22:42,630
+اللي هي semi-solid media inculationاللي هي الـ
+
+302
+00:22:42,630 --> 00:22:46,130
+most common the most commonly used test for
+
+303
+00:22:46,130 --> 00:22:49,770
+motility in microbiology لأ هي أكثرها شيوعا
+
+304
+00:22:49,770 --> 00:22:53,890
+واستخداما في معمل الـ microbiology عشان أحدد هل
+
+305
+00:22:53,890 --> 00:22:58,410
+البكتيريا motile or non-motile بقوللي it depends
+
+306
+00:22:58,410 --> 00:23:01,850
+on the ability of motile bacteria to move through
+
+307
+00:23:01,850 --> 00:23:06,570
+semi-solid media هي بتعتمد على قدرة المكتيريا على
+
+308
+00:23:06,570 --> 00:23:11,770
+الحركة في semi-solid mediaالميديا قلنا هي وسط غضاء
+
+309
+00:23:11,770 --> 00:23:16,950
+لنمو البكتيريا بقسم الميديا حسب نسبة الأجار طبعا
+
+310
+00:23:16,950 --> 00:23:20,650
+الأجار اللي يعتبر هو solidifying agent اللي هو
+
+311
+00:23:20,650 --> 00:23:27,930
+بيخل الوسط صلب ممكن الميديا تكون solid و ممكن تكون
+
+312
+00:23:27,930 --> 00:23:32,680
+semi solid و ممكن تكون liquidلما تكون solid بتكون
+
+313
+00:23:32,680 --> 00:23:37,920
+نسبة الأجار من 1.5 لـ 2% أجار لو كانت semi-solid
+
+314
+00:23:37,920 --> 00:23:41,460
+اللي أنا بستخدمها في فحص الـ Motility نسبة الأجار
+
+315
+00:23:41,460 --> 00:23:48,380
+4 من 10% أجار لكن بالنسبة لو كانت liquid أكيد
+
+316
+00:23:48,380 --> 00:23:53,920
+هتكون no أجار لأنه ما صارش هي broth ضلت سائلة ما
+
+317
+00:23:53,920 --> 00:23:58,180
+اتصلبت يبقى توزيع أو تقسيم الـ media على حسب اللي
+
+318
+00:23:58,180 --> 00:24:03,490
+هو نسبة الأجار solidتكون نسبة الأجار من 1.5% لـ 2%
+
+319
+00:24:03,490 --> 00:24:09,550
+سيمي سوليد 4 من 10% Liquid مافيش أي نسبة أجار Zero
+
+320
+00:24:09,550 --> 00:24:16,710
+كيف أنا بدي أعمل الفحص قبل ما نحكي على كيف أنا ال
+
+321
+00:24:16,710 --> 00:24:19,890
+procedure لهذا الفحص فحص ال modality لازم يكون
+
+322
+00:24:19,890 --> 00:24:24,970
+عندي أنا سيمي سوليد 100 يعني نسبة الأجار 4 من 10%
+
+323
+00:24:24,970 --> 00:24:29,520
+لأنه أنا لو استخدمتsolid media أكيد البكتيريا مش
+
+324
+00:24:29,520 --> 00:24:34,240
+هتتحرك هيكون صلب لو استخدمت liquid هتتحرك وتنتشر
+
+325
+00:24:34,240 --> 00:24:38,460
+في كل tube ومش هعرف هل هي true movement او
+
+326
+00:24:38,460 --> 00:24:42,520
+Brownian movement اللي هي حركة جزيئات السائل فلازم
+
+327
+00:24:42,520 --> 00:24:47,440
+انا استخدم semi solid mediaطبعاً أنا هاي الـ Semi
+
+328
+00:24:47,440 --> 00:24:51,780
+-solid media بصوبها في تيوب تمام احنا قلنا نحضر ال
+
+329
+00:24:51,780 --> 00:24:56,660
+media بصوبها ممكن يكون في قبلات لكن في ال semi
+
+330
+00:24:56,660 --> 00:25:02,480
+-solid media بصوبها في تيوب بتركها تتصلب فتعطيني
+
+331
+00:25:02,480 --> 00:25:07,760
+اللي هي step هنتعرف عليها بالتفصيل في المعمل الجاي
+
+332
+00:25:07,760 --> 00:25:09,840
+هيك بتكون الشكل التيوب
+
+333
+00:25:16,030 --> 00:25:21,030
+التيوب بصب ال media فيه هلاقيه زي الجل متصلب هاي
+
+334
+00:25:21,030 --> 00:25:26,250
+ال .. هذا الاشي بسميه stabbing لأني هعمل طعن في ال
+
+335
+00:25:26,250 --> 00:25:31,670
+tube هنتعرف هلأ بالتفصيل كيف خطوات الشغل خطوات
+
+336
+00:25:31,670 --> 00:25:36,830
+الشغل بقولي انا بستخدم sterile needle اش يعني
+
+337
+00:25:36,830 --> 00:25:41,450
+sterile needle بالنسبة ل ال necrum loop ال necrum
+
+338
+00:25:41,450 --> 00:25:44,750
+loop بقولي احنا قولنا في عندي loop ال loop ممكن
+
+339
+00:25:44,750 --> 00:25:49,790
+تكونالانيكولاتينج لوب الانيكولاتينج لوب high فيه
+
+340
+00:25:49,790 --> 00:25:58,290
+بكون إلها حلقة لكن الانيكولاتينج needle ال loop ما
+
+341
+00:25:58,290 --> 00:26:03,890
+إلها حلقة يبقى ال loop high بسميها انيكولاتينج
+
+342
+00:26:03,890 --> 00:26:07,310
+loop فيه موجود فيها حلقة high ما بستخدمها في فحص
+
+343
+00:26:07,310 --> 00:26:12,170
+ال motility لكن الانيكولاتينج needleهي ال needle
+
+344
+00:26:12,170 --> 00:26:16,190
+بستخدمها في فحص ال motility باخد اللي هو ال colony
+
+345
+00:26:16,190 --> 00:26:22,670
+من الطبق وبعدين هزرعها في هايلة يوم طيب الخطوة
+
+346
+00:26:22,670 --> 00:26:25,830
+التانية يبقى عرفنا إيش يعني ال needle اللي هي loop
+
+347
+00:26:25,830 --> 00:26:29,510
+لكن مايلها حلقة بستخدمها في فحص اللي هو ال
+
+348
+00:26:29,510 --> 00:26:33,980
+motility أو أي فحص فيه اللي هو stabbingباخد اي
+
+349
+00:26:33,980 --> 00:26:37,740
+كولوني من الارجانيزم اللي انا ببحث هل هو motile او
+
+350
+00:26:37,740 --> 00:26:41,900
+non motile الخطوة التانية Stab quickly a tube of
+
+351
+00:26:41,900 --> 00:26:45,860
+semi-solid media بقولي اعملي Stabbing ايش يعني
+
+352
+00:26:45,860 --> 00:26:48,580
+Stabbing يعني اعملي طعنة في ال tube اللي انت
+
+353
+00:26:48,580 --> 00:26:51,800
+جهزتيها سيمي سوليد ميديا الميديا اللي بتحتوي على
+
+354
+00:26:51,800 --> 00:26:56,720
+أربع من عشرة بالمية أجاركيف بدي أعمل؟ بقولنا
+
+355
+00:26:56,720 --> 00:27:00,440
+بتجيبي الـ Nicolating Needle بعد ما أخدتي من
+
+356
+00:27:00,440 --> 00:27:05,080
+البكتيريا من الطبق اللي بدك تشوفه هل هو البكتيريا
+
+357
+00:27:05,080 --> 00:27:08,440
+motile أو non-motile بتدخلي الـ Nicolating Needle
+
+358
+00:27:08,440 --> 00:27:14,220
+و بتعملي طعنة تمام بسرعة بتعملي طعنة في التيوم
+
+359
+00:27:14,220 --> 00:27:18,560
+طبعا مش للآخر تقريبا بتوصل إلى التلتان و بتعملي
+
+360
+00:27:18,560 --> 00:27:23,690
+طعنة و بتطلعي بسرعةبعد ما اتخلصى بتعملى incubation
+
+361
+00:27:23,690 --> 00:27:28,050
+the semi solid media لمدة 24 ساعة في ال incubator
+
+362
+00:27:28,050 --> 00:27:31,690
+بدي اعمل incubation بحطه في ال incubator لمدة 24
+
+363
+00:27:31,690 --> 00:27:38,290
+ساعة بعد 24 ساعة بقرأ النتيجة قبل ما انا اعمل
+
+364
+00:27:38,290 --> 00:27:43,450
+stabbing لما افتحت ال tube طبعا لازم احنا من ال
+
+365
+00:27:43,450 --> 00:27:48,030
+aseptic technique اعقم فوهة ال tube وبعد ما اخلص
+
+366
+00:27:48,030 --> 00:27:52,690
+نفس الاشي اعقم فوهة ال tubeهذا من الـ Aseptic
+
+367
+00:27:52,690 --> 00:27:58,430
+technique بالنسبة للنتيجة النتيجة بتكون بعد 24
+
+368
+00:27:58,430 --> 00:28:02,530
+ساعة كيف بدي أفسر النتيجة بقولي pattern of growth
+
+369
+00:28:02,530 --> 00:28:06,550
+of motile organism the intermediate is turbid with
+
+370
+00:28:06,550 --> 00:28:09,730
+the growth of organism which has moved away from
+
+371
+00:28:09,730 --> 00:28:13,870
+the stop line بقولي هان لو كانت البكتيريا motile
+
+372
+00:28:13,870 --> 00:28:19,330
+كيف بدي أعرف بقولي أنه ان نمو البكتيريا بكونبتتحرك
+
+373
+00:28:19,330 --> 00:28:23,870
+على خط الزراع و كمان بلاحظ ان في انها تحركت كمان
+
+374
+00:28:23,870 --> 00:28:28,170
+بعيدا عن خط الزراع يبقى لو كانت motile ال media
+
+375
+00:28:28,170 --> 00:28:31,270
+هتكون اكيد turbid لأنها هتنمو البكتيريا سواء كانت
+
+376
+00:28:31,270 --> 00:28:35,150
+motile او non motile هيكون في عندي turbidity لكن
+
+377
+00:28:35,150 --> 00:28:40,070
+كيف بدي احدد هل هي motile او non motile بقوللي انه
+
+378
+00:28:40,070 --> 00:28:44,350
+في ال motile بتتحرك بعيدا عن خط الزراع لكن في ال
+
+379
+00:28:44,350 --> 00:28:48,300
+non motileفقط بلاقي الـ turbidity على خط الزراعة
+
+380
+00:28:48,300 --> 00:28:52,200
+لأنه ماقدرت تتحرك بعيدا فقط دلت على خط الزراعة
+
+381
+00:28:52,200 --> 00:28:57,300
+لأنه نمت بكتيريا pattern هيو has moved away from
+
+382
+00:28:57,300 --> 00:29:00,820
+the step line الموتالي بتتحرك بعيدا عن خط الزراعة
+
+383
+00:29:00,820 --> 00:29:03,920
+ال step line يعني خط الزراعة pattern of growth of
+
+384
+00:29:03,920 --> 00:29:08,860
+a non-motile organism only the step line is turbid
+
+385
+00:29:08,860 --> 00:29:12,640
+with growth هلاقي فقط انه مو ال turbidity فقط على
+
+386
+00:29:12,640 --> 00:29:17,130
+خط الزراعةبعض الأحيان ممكن انا ما اشوف هاي الـ
+
+387
+00:29:17,130 --> 00:29:21,910
+turbidity فممكن اضيف color indicator indicator كي
+
+388
+00:29:21,910 --> 00:29:24,730
+اشوف على ال media اضيفه قبل و انا بحضر ال media
+
+389
+00:29:24,730 --> 00:29:29,250
+ممكن اضيف indicator هذا ال indicator هيتلون ال
+
+390
+00:29:29,250 --> 00:29:33,130
+turbidity تبع البكتيريا بلون ال indicator وهك انا
+
+391
+00:29:33,130 --> 00:29:35,870
+بقدر اشوف ال turbidity بشكل واضح
+
+392
+00:29:38,670 --> 00:29:41,930
+يبقى الـ Motile و الـ Non-motile هيكون فيه تربط
+
+393
+00:29:41,930 --> 00:29:46,610
+لكن الفرق أن الـ Motility هتلاقي التربط بعيدا عن
+
+394
+00:29:46,610 --> 00:29:50,670
+خط الزراعة لكن الـ Non-motile ما بتقدر تتحرك فقط
+
+395
+00:29:50,670 --> 00:29:52,690
+هلاقي على خط الزراعة
+
+396
+00:30:01,190 --> 00:30:06,690
+هنقل خطوات بقوللي طبعا اول اشي بعقم بفتح الفوهة
+
+397
+00:30:06,690 --> 00:30:12,790
+للتيوب بعقم الفوهة بكون قابلها ماخدة طبعا اللي هي
+
+398
+00:30:12,790 --> 00:30:19,350
+big colony of bacteria ببيه ال needle بال needle
+
+399
+00:30:19,350 --> 00:30:23,830
+زي ما احنا شايفين مافيه لها حلقة بدخل بسرعة زي ما
+
+400
+00:30:23,830 --> 00:30:29,290
+احنا ملاحظين في المنتصف فقط لاتلتان بعدين باطلع
+
+401
+00:30:29,810 --> 00:30:35,190
+بعقم طبعا اللى هو ال needle و بعقم الفوهة و بحطها
+
+402
+00:30:35,190 --> 00:30:43,710
+في ال incubator هذه النتيجة هلأ بنشوف هذه بالنسبة
+
+403
+00:30:43,710 --> 00:30:48,330
+ل semisolid media with tetrazolium chloride اللى
+
+404
+00:30:48,330 --> 00:30:50,990
+هو ال color indicator احنا قلنا ممكن عشان اقدر ان
+
+405
+00:30:50,990 --> 00:30:55,800
+انا اشوف بشكل واضح ممكن استخدم indicatorتمام اضيفه
+
+406
+00:30:55,800 --> 00:30:59,760
+على ال media عشان اشوف انمو العكارة بشكل واضح هشوف
+
+407
+00:30:59,760 --> 00:31:03,560
+العكارة باللون الأحمر يبقى Tetrazolium chloride
+
+408
+00:31:03,560 --> 00:31:07,660
+يعتبر color indicator هذا مصطلح مهم ملاحظ في
+
+409
+00:31:07,660 --> 00:31:12,580
+الصورة انه انا هان اتلونت كل ال tube باللون الأحمر
+
+410
+00:31:12,580 --> 00:31:16,240
+يبقى العكارة موجودة على خط الزراع وعلى اتحركت
+
+411
+00:31:16,240 --> 00:31:20,660
+بعيدا عن خط الزراع يبقى هي positive لكن هان فقط
+
+412
+00:31:20,870 --> 00:31:24,630
+اللي العكارة فقط كانت على خط الزراعة اللي اتلون
+
+413
+00:31:24,630 --> 00:31:28,410
+باللون الأحمر خط الزراعة يبقى non motile هنبقى
+
+414
+00:31:28,410 --> 00:31:31,710
+نلاحظ انه اتحركت ايضا بعيد عن خط الزراعة يبقى هي
+
+415
+00:31:31,710 --> 00:31:36,290
+positive هك بنكون خلصنا ال bacterial motility
+
+416
+00:31:36,290 --> 00:31:38,910
+السلام عليكم ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/DBNaI7FWU-U_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/DBNaI7FWU-U_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..39f2b6ca95fc0caaaaa98fb325de9e7e9dffdb3c
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/DBNaI7FWU-U_postprocess.srt
@@ -0,0 +1,1348 @@
+1
+00:00:00,470 --> 00:00:04,270
+morphology of bacteria the basis for studying is
+
+2
+00:00:04,270 --> 00:00:08,070
+to study the morphology and structure of bacteria
+
+3
+00:00:08,070 --> 00:00:11,990
+قلنا هدفي من الصباغة أني أنا أتعرف على شكل
+
+4
+00:00:11,990 --> 00:00:16,170
+البكتيريا وقلنا أشكال البكتيريا ممكن تكون
+
+5
+00:00:16,170 --> 00:00:21,550
+spherical كراوية كوكس or كوكاي ممكن تكون rod shape
+
+6
+00:00:21,550 --> 00:00:26,090
+اللي هي عصوية basilas or basilae يبقى أشكال
+
+7
+00:00:26,090 --> 00:00:30,470
+البكتيريا ممكن تكون كوكاي وممكن تكون basilaeقلنا
+
+8
+00:00:30,470 --> 00:00:33,570
+على الـ Simple Stain هدفي اني انا اتعرف على الشاب
+
+9
+00:00:33,570 --> 00:00:37,650
+و الـ arrangement للبكتيريا اتعرفنا على الشاب of
+
+10
+00:00:37,650 --> 00:00:40,690
+بكتيريا طب شو بالنسبة لالـ arrangement؟ الـ
+
+11
+00:00:40,690 --> 00:00:45,210
+arrangement هو ترتيب الخلايا البكتيرية بيعتمد الـ
+
+12
+00:00:45,210 --> 00:00:48,730
+arrangement على الـ plan of division على مستوى
+
+13
+00:00:48,730 --> 00:00:52,990
+الانقسام seen in the organism bacteria may also
+
+14
+00:00:52,990 --> 00:00:58,660
+have a specific arrangement of the cellالمستوى
+
+15
+00:00:58,660 --> 00:01:04,420
+الانقسام عشان نفهم اكتر لو انقسمت الخلية البكتيرية
+
+16
+00:01:04,420 --> 00:01:08,440
+وكان مستوى الانقسام اذا ملاحظين كان vertical بشكل
+
+17
+00:01:08,440 --> 00:01:14,420
+عمودي وعططن خليتين هدول الخليتين دلهم ملتسقين مع
+
+18
+00:01:14,420 --> 00:01:19,120
+بعض ما انفصلوا وكان الشاب بالاصل ككاي يبقى بسمي ال
+
+19
+00:01:19,120 --> 00:01:24,460
+arrangement دبلو بما ان الشكل ككاي يبقى دبلو ككاي
+
+20
+00:01:24,460 --> 00:01:29,940
+لو كان الشكل في الأصل بصلاييبقى بسمي الاخر شئ
+
+21
+00:01:29,940 --> 00:01:34,200
+البكتيريا diplo بصلاي diplo الarrangement و بصلاي
+
+22
+00:01:34,200 --> 00:01:40,380
+الشكل طيب لو كان مستوى الانقسام بشكل عمودي لكن كان
+
+23
+00:01:40,380 --> 00:01:45,700
+continuous ضال مستمر مش فقط خليتين كان كوّن chain
+
+24
+00:01:45,700 --> 00:01:50,360
+of كُكّال cell كوّن سلسلة من الخلايا البكتيرية و
+
+25
+00:01:50,360 --> 00:01:54,740
+كانت الشكل في الأصل كُكّا يبقى بسمي الarrangement
+
+26
+00:01:54,740 --> 00:01:59,160
+streptoوالشياب ككاي يبقى بالنهاية اسمها
+
+27
+00:01:59,160 --> 00:02:03,700
+streptococci طب لو كان الشكل في الأصل بسلاي وكانت
+
+28
+00:02:03,700 --> 00:02:08,580
+انقسمت بشكل عمودي لكن ضالوا continually بسميها
+
+29
+00:02:08,580 --> 00:02:13,380
+streptobacillary بالنسبة لل plan of division لو
+
+30
+00:02:13,380 --> 00:02:17,320
+كان عندي مستوى الانقسام vertical و horizontal كان
+
+31
+00:02:17,320 --> 00:02:23,470
+في عندي طول و عرض بسمي الشكل tetradلو كان عندى
+
+32
+00:02:23,470 --> 00:02:28,190
+مستوى الانقسام تلاتة طول و عرض و ارتفاع بسمى الـ
+
+33
+00:02:28,190 --> 00:02:32,270
+Arrangement Sarcina احنا بنعرف او قولنا في معمل
+
+34
+00:02:32,270 --> 00:02:37,450
+الـ Microscope الصورة اللى بيعطينى ياها الـ Light
+
+35
+00:02:37,450 --> 00:02:41,490
+Microscope هي Virtual صورة افتراضية بتعطينى فقط
+
+36
+00:02:41,490 --> 00:02:45,690
+طول و عرض فالـ Sarcina انا فيه عندى مستوى الانقسام
+
+37
+00:02:45,690 --> 00:02:49,790
+كان طول و عرض و ارتفاعيبقى ما بقدر أتعرف على
+
+38
+00:02:49,790 --> 00:02:53,810
+الساركينة في الـ Light Microscope بحتاج إلى المشهر
+
+39
+00:02:53,810 --> 00:02:59,590
+التشريحي حتى أني أنا أشوف الساركينة لو انقسمت بشكل
+
+40
+00:02:59,590 --> 00:03:04,510
+Randomly، بشكل عشوائي، بسميها Staphylococcus ولو
+
+41
+00:03:04,510 --> 00:03:08,970
+كان الشكل كوكاي، بسميها Staphylococcus بشكل ممقوضي
+
+42
+00:03:08,970 --> 00:03:12,770
+لعينة، بنسميها ممقوضي ممكن يكون ما عندي
+
+43
+00:03:12,770 --> 00:03:15,770
+Arrangement تظلها Single سواء Single كوكاي أو
+
+44
+00:03:15,770 --> 00:03:19,660
+Single بصل Lightبنحكي non-arrangement يبقى هذا
+
+45
+00:03:19,660 --> 00:03:23,280
+بالنسبة للـarrangement of bacteria نرجع على ال
+
+46
+00:03:23,280 --> 00:03:27,160
+slide Diplococci are formed when the plane of
+
+47
+00:03:27,160 --> 00:03:30,280
+division is vertical بقولنا بتتكون إذا كان مستوى
+
+48
+00:03:30,280 --> 00:03:34,420
+الانقسام عمودي والنتيجة كان عندي two coccal cells
+
+49
+00:03:34,420 --> 00:03:38,980
+خليتين كرويتين لم ينفصلوا don't completely
+
+50
+00:03:38,980 --> 00:03:43,000
+separate from each other ما انفصلوا عن بعضبقولنا
+
+51
+00:03:43,000 --> 00:03:45,640
+if the cell divide in the vertical plane
+
+52
+00:03:45,640 --> 00:03:49,660
+continually and the cell don't separate لو كان
+
+53
+00:03:49,660 --> 00:03:54,140
+مستوى الانقسام عمودي لكن بشكل مستمر وهي الخلايا ما
+
+54
+00:03:54,140 --> 00:03:58,020
+انفصلت وكونت chain of cocktail cell بسمي أنا
+
+55
+00:03:58,020 --> 00:04:02,820
+الخلايا streptococci أو streptobacilli حسب الشكل
+
+56
+00:04:02,820 --> 00:04:06,740
+other arrangement لو انقسمت بشكل ربعي بسميها
+
+57
+00:04:06,740 --> 00:04:10,870
+tetrad كان مستوى الانقسام vertical و horizontalلو
+
+58
+00:04:10,870 --> 00:04:14,910
+كان المستوى الانقسام طوله عرض وارتفاع بشكل ثمانيات
+
+59
+00:04:14,910 --> 00:04:18,010
+بسميه ساركينة وقولنا الساركينة ما بشوفها في ال
+
+60
+00:04:18,010 --> 00:04:21,990
+light microscope أخر الشيء لو كان بشكل randomly أو
+
+61
+00:04:21,990 --> 00:04:26,410
+cluster بسمي ال arrangement استفيله كُكّة يبقى هذا
+
+62
+00:04:26,410 --> 00:04:31,750
+بالنسبة ليه ال arrangement ال simple stand بتعرف
+
+63
+00:04:31,750 --> 00:04:34,890
+من خلال علشاب و ال arrangement الشاب ممكن يكون
+
+64
+00:04:34,890 --> 00:04:38,110
+كُكّة يبص اللهي ال arrangement حسب مستوى الانقسام
+
+65
+00:04:38,110 --> 00:04:42,960
+إذا كان vertical دبلوVertical Continually Strictly
+
+66
+00:04:42,960 --> 00:04:46,220
+Randomly بشكل عشوائي بتكون Stiff ممكن يكون No
+
+67
+00:04:46,220 --> 00:04:50,480
+Arrangement ممكن يكون Other زي الـ Tetrad والسركي
+
+68
+00:04:50,480 --> 00:04:56,320
+هذا بالنسبة للشاب والـArrangement خلصنا من قلنا أو
+
+69
+00:04:56,320 --> 00:05:00,060
+اتكلمنا على الهدف أو الـPrincipal من الـSimple
+
+70
+00:05:00,060 --> 00:05:03,400
+Plan الـObjective هدف الـSimple Plan هلأ نيجي
+
+71
+00:05:03,400 --> 00:05:08,290
+لخطوات العمل أشهر الـProcedureاللي انا بشتغلها او
+
+72
+00:05:08,290 --> 00:05:12,350
+الخطوات حتى اشتغل الـ Stain Symbol Staining
+
+73
+00:05:12,350 --> 00:05:16,350
+Protocol in Microbiology Lab أول خطوة اللي هي الـ
+
+74
+00:05:16,350 --> 00:05:20,990
+Bacterial Sphere Preparation بدي أحضر البكتيريا
+
+75
+00:05:20,990 --> 00:05:25,490
+بقولنا تحضير البكتيريا بيعتمد على النوع الوسط اللي
+
+76
+00:05:25,490 --> 00:05:29,850
+نمت عليه البكتيريا ممكن تنمو على ميديا وسط هذا
+
+77
+00:05:29,850 --> 00:05:34,670
+الوسط ممكن يكون Agar، Agar يعني صلبةوقتها انا بصب
+
+78
+00:05:34,670 --> 00:05:39,950
+ال media في بلات في أطباق بطري في بلات وممكن تكون
+
+79
+00:05:39,950 --> 00:05:44,230
+سائل liquid او بنسميها broth broth يعني liquid
+
+80
+00:05:44,230 --> 00:05:49,390
+سائل ووقتها بصبها في tube طريقة تتحضير البكتيريا
+
+81
+00:05:49,390 --> 00:05:53,790
+بتختلف حسب إذا كان الوسط أجار أو كان broth لو كان
+
+82
+00:05:53,790 --> 00:05:58,490
+أجار طبعا أكيد هصبه في بلات بجيب ال slide و بضيف
+
+83
+00:05:58,490 --> 00:06:01,950
+drop من ال normal saline ال normal saline محلول
+
+84
+00:06:01,950 --> 00:06:08,790
+ملحيتركيزه 85% من 100% زائد Loop Full of Bacteria
+
+85
+00:06:08,790 --> 00:06:14,210
+باخد مسحة من الكلوني من الطبق بستيرايل Loop تروري
+
+86
+00:06:14,210 --> 00:06:17,990
+أنا أستخدم استيرايل Loop تكون Aseptic Technique
+
+87
+00:06:17,990 --> 00:06:22,550
+أمنع ال Contamination لو كان نيكروم Loop عندي بتم
+
+88
+00:06:22,550 --> 00:06:27,570
+تعقيمه باستخدام اللهبكاتبيه لانا كاتبة من أوسان
+
+89
+00:06:27,570 --> 00:06:31,370
+اللى هو يفضل اني انا اخد isolated colony ليش
+
+90
+00:06:31,370 --> 00:06:35,270
+isolated colony؟ عشان اضمن ان كل البكتيريا أصلها
+
+91
+00:06:35,270 --> 00:06:39,870
+واحد بالتالي هيكون شكلها واحد بعمل بعد ما أخدت
+
+92
+00:06:39,870 --> 00:06:42,870
+اللوب full of bacteria بعمل mix مع ال normal
+
+93
+00:06:42,870 --> 00:06:48,130
+saline و بروح بفرد باللوب و بحاول اني انا افردها
+
+94
+00:06:48,130 --> 00:06:53,790
+على كل slide تمام؟ هيك انا خلصت من اول طريقة اللى
+
+95
+00:06:53,790 --> 00:06:58,920
+هي الاسمية preparationطيب انا قولت بدي استخدم او
+
+96
+00:06:58,920 --> 00:07:02,680
+اني انا لازم اخد isolated colony ايش هي ال
+
+97
+00:07:02,680 --> 00:07:05,960
+isolated colony؟ انا في عندي اكتر من طريقة لزراعة
+
+98
+00:07:05,960 --> 00:07:10,060
+هنتعرف عليها في معمل ال media preparation ضمن طرق
+
+99
+00:07:10,060 --> 00:07:13,800
+الزراعة طريقة ال quaterner طريقة الأربع هاي
+
+100
+00:07:13,800 --> 00:07:17,740
+الطريقة الهدف منها احصل على isolated colony انا
+
+101
+00:07:17,740 --> 00:07:21,400
+بزرع الربع الأول تمام بجيب البكتيريا اللى انا بدي
+
+102
+00:07:21,400 --> 00:07:28,070
+ازراحهاباستخدام اللوب وبزرع في الربع الأول بعدها
+
+103
+00:07:28,070 --> 00:07:31,070
+بستخدم اللوب تاني إذا كنت بستخدم ال plastic loop
+
+104
+00:07:31,070 --> 00:07:34,970
+بستخدم اللوب الجديد وإذا بستخدم نيكروم لوب بروح
+
+105
+00:07:34,970 --> 00:07:39,930
+بعقم هذا اللوب ما برجع باخد بكتيريا تانية فقط باخد
+
+106
+00:07:39,930 --> 00:07:46,490
+من وين باخد من هذا الربع و بطلع بخطين هيك أنا في
+
+107
+00:07:46,490 --> 00:07:51,530
+هذه الطريقة بكون خففةبعدها بستخدم لو بيشزير اذا
+
+108
+00:07:51,530 --> 00:07:55,410
+plastic وبزرع الربع التالت وهكذا في الرابع الرابع
+
+109
+00:07:55,410 --> 00:07:59,550
+بهذه الطريقة بخفف ال colony بحاول اعزل ال colony
+
+110
+00:07:59,550 --> 00:08:02,790
+عن بعض و احصل على isolated colony في هن دي انا
+
+111
+00:08:02,790 --> 00:08:06,930
+صورة هذه الصورة اذا ملاحظين الربع الاول ثق و
+
+112
+00:08:06,930 --> 00:08:10,350
+المستعمرات فوق بعض الربع التاني اخف من الربع الاول
+
+113
+00:08:10,350 --> 00:08:14,560
+بدأت شوية المستعمرات تتبعد عن بعضالربع التالت ايضا
+
+114
+00:08:14,560 --> 00:08:18,040
+اخف من التاني في الرابع من اللاحظ انه ال colony
+
+115
+00:08:18,040 --> 00:08:21,740
+معزولة عن بعض فباخد انا هاي colony هيك بضمن ان
+
+116
+00:08:21,740 --> 00:08:26,220
+اصلها خلية بكتيريا واحدة يعني كل شكل الخلايا هيكون
+
+117
+00:08:26,220 --> 00:08:30,120
+نفس الشيء طيب ايش بالنسبة ل ال normal saline
+
+118
+00:08:30,120 --> 00:08:33,500
+اتكلمنا عن البكتيريا نيجي نحكي على ال normal
+
+119
+00:08:33,500 --> 00:08:37,680
+saline ال normal saline لازم يكون تركيزه 85% من
+
+120
+00:08:37,680 --> 00:08:43,500
+100% كيف بدأ احضر هذا التركيزأو ايش معنى 85 من 100
+
+121
+00:08:43,500 --> 00:08:48,900
+%؟ معنى انه 85 من 100 جرام من الـ Normal Saline هو
+
+122
+00:08:48,900 --> 00:08:53,700
+بيكون Salt زي الملح مدوبة في 100 مل من الـ
+
+123
+00:08:53,700 --> 00:08:57,720
+Distilled Water طيب لو أنا بدي كمية أكتر بروح بدرب
+
+124
+00:08:57,720 --> 00:09:02,700
+درب تدباد و ليه؟ على سبيل المثال بدي 1000 مل باخد
+
+125
+00:09:02,700 --> 00:09:07,100
+8.5 جرام من الـ Normal Saline و بدوبها ب1000 مل من
+
+126
+00:09:07,100 --> 00:09:12,290
+الـ Distilled Waterننتبه هنا إنه أنا هذا ال normal
+
+127
+00:09:12,290 --> 00:09:15,490
+saline صلب فانا استخدمت هذه الطريقة لكن لما كنا
+
+128
+00:09:15,490 --> 00:09:22,510
+نحضر كحول 70% بحط 70 مل كحول و بكمل على المية
+
+129
+00:09:22,510 --> 00:09:26,450
+distal water يعني بالنهاية بيطلع ال distal water
+
+130
+00:09:26,450 --> 00:09:29,910
+تلاتين لكن هنا أنا حطيت المية كلها distal water
+
+131
+00:09:29,910 --> 00:09:34,910
+لأنه ال normal saline هنا صلبطيب، شو وظيفة الـ
+
+132
+00:09:34,910 --> 00:09:37,450
+Normal Saline؟ احنا كاتبينها إن Normal Saline
+
+133
+00:09:37,450 --> 00:09:41,030
+بحافظ لي على الضغط الأسموازي لأنه لو كان الـ
+
+134
+00:09:41,030 --> 00:09:45,550
+Normal Saline أكتر من 85% من 100% هيصير في عندي
+
+135
+00:09:45,550 --> 00:09:49,950
+Shrinking للخلايا لو كان أقل من 85% من 100% هيصير
+
+136
+00:09:49,950 --> 00:09:55,210
+في عندي Swallowing للخلاياسبب تاني إن أنا استخدمت
+
+137
+00:09:55,210 --> 00:09:58,270
+الـ Normal Saline في هذه الطريقة لأن الكولوني
+
+138
+00:09:58,270 --> 00:10:02,510
+بتكون جافة فانا بدي إشي سائل أني أحاول أوزعها على
+
+139
+00:10:02,510 --> 00:10:05,690
+الـ Slime يبقى عشان يحفظلي على الـ Osmotic
+
+140
+00:10:05,690 --> 00:10:09,730
+Pressure وسبب تاني لأن الكولوني جافة فبدي إشي
+
+141
+00:10:09,730 --> 00:10:14,510
+أحاول يسهل عليّ توزيع الخلايا البكتيرية على الـ
+
+142
+00:10:14,510 --> 00:10:18,590
+Slime هيك خلصنا من طريقة الأجارة طيب لو كان الوسط
+
+143
+00:10:18,590 --> 00:10:22,780
+الـ Broth لو كان الوسط سائلبقول نهار مش محتاجة أنا
+
+144
+00:10:22,780 --> 00:10:27,500
+normal saline لأن هو سائل فقط بضيف drop من ال
+
+145
+00:10:27,500 --> 00:10:30,980
+bacterial broth أو ال bacterial suspension نفس
+
+146
+00:10:30,980 --> 00:10:33,780
+المعنى ال bacterial broth نفس المعنى ال bacterial
+
+147
+00:10:33,780 --> 00:10:38,180
+suspension bacterial broth معناها ميديا سائلة
+
+148
+00:10:38,180 --> 00:10:42,220
+مزروعة فيها بكتيريا bacterial suspension اللي هو
+
+149
+00:10:42,220 --> 00:10:47,100
+سائل ممكن يكون normal saline ممكن يكون ميديا سائلة
+
+150
+00:10:47,100 --> 00:10:51,310
+معلقة فيه بكتيرياوبفرد باستخدام اللوب أو في الاصل
+
+151
+00:10:51,310 --> 00:10:54,410
+ممكن ما اخد ما اخد في الادروبر اخد ال bacterial
+
+152
+00:10:54,410 --> 00:10:58,290
+suspension باستخدام اللوب احاول افرد حوالين لسلون
+
+153
+00:10:58,290 --> 00:11:02,930
+هان بهذه الطريقة ما بحط cover slip ننتبه في طريقة
+
+154
+00:11:02,930 --> 00:11:06,410
+ال wet mouth هديك ال living understand ال organism
+
+155
+00:11:06,410 --> 00:11:09,670
+بنستخدم ال wet mouth بنستخدم ال cover slip هان ما
+
+156
+00:11:09,670 --> 00:11:14,290
+بنحط cover slip هيك خلصنا من أول خطوة اللي هي ال
+
+157
+00:11:14,290 --> 00:11:18,630
+bacterial اسمه preparationلكن في بعض الأحيان ممكن
+
+158
+00:11:18,630 --> 00:11:23,190
+يصير في عندي مشاكل في السمير ممكن السمير يكون عندى
+
+159
+00:11:23,190 --> 00:11:27,210
+thick سمير وممكن يكون thin سمير طب هي مشكلة؟ اه
+
+160
+00:11:27,210 --> 00:11:32,790
+مشكلة أكيد thick سمير لو كان انا استخدمت او عملت
+
+161
+00:11:32,790 --> 00:11:36,270
+thick سمير يعني هتكون الخلايا أو الخلايا البكتيرية
+
+162
+00:11:36,270 --> 00:11:39,950
+فوق بعض فبالتالي مش هتعرفي اتميزي الشاب و ال
+
+163
+00:11:39,950 --> 00:11:44,860
+arrangement عشان نحل هذه المشكلةمنضيف ومنخفف،
+
+164
+00:11:44,860 --> 00:11:48,620
+مايعني منخفف؟ يعني منضيف another drop of normal
+
+165
+00:11:48,620 --> 00:11:54,220
+saline تنسمير يعني الخلايا بتكون قليلة في هذا ال
+
+166
+00:11:54,220 --> 00:11:57,860
+slide عشان أني أنا أحل هذه المشكلة، أش بعمل؟ take
+
+167
+00:11:57,860 --> 00:12:01,500
+another loop full of bacteria، بضيف مسحة أخرى من
+
+168
+00:12:01,500 --> 00:12:04,340
+البكتيريا، يبقى خلصنا أول خطوة اللي هي ال
+
+169
+00:12:04,340 --> 00:12:08,560
+bacterial smear preparation الخطوة التانية ال air
+
+170
+00:12:08,560 --> 00:12:13,350
+dryingبنخلّيها تنشف الـ slide في الهواء أو ممكن
+
+171
+00:12:13,350 --> 00:12:17,450
+نحطها في الـ incubator 37 درجة سيليزيا بيجففها
+
+172
+00:12:17,450 --> 00:12:22,190
+بشكل أسرع طب ليش أنا بدي أعمل هذه الخطوة؟ بقولنا
+
+173
+00:12:22,190 --> 00:12:26,910
+to prevent lysis حتى أني أمنع تحلل الخلايا طب عشان
+
+174
+00:12:26,910 --> 00:12:30,490
+نفهم أكتر ليش أنا بدي أمنع أو كيف بدي أمنع تحلل
+
+175
+00:12:30,490 --> 00:12:34,630
+الخلايا إذا أنا إذا أنا اتبعت أو عملت air drying
+
+176
+00:12:34,630 --> 00:12:38,710
+لنحكي عن الخطوة التالتة اللي هي الـ heat fixation
+
+177
+00:12:39,310 --> 00:12:44,270
+الـ heat fixation في هذه الخطوة بمرر الـ slide على
+
+178
+00:12:44,270 --> 00:12:49,250
+اللهب بسرعة تلت مرات يبقى هتتعرض ال slide أو
+
+179
+00:12:49,250 --> 00:12:53,750
+البكتيريا لحرارة في هذه الخطوة لو أنا ماعملت خطوة
+
+180
+00:12:53,750 --> 00:12:57,350
+الـ air drying و روحت على خطوة الـ heat fixation
+
+181
+00:12:57,350 --> 00:13:00,290
+هيك بيكون فيه على ال slide فيه ميه لأنه انا مانا
+
+182
+00:13:00,290 --> 00:13:05,770
+جففتها ميه زاد حرارة هيصير عندي license للخلايا
+
+183
+00:13:05,770 --> 00:13:10,250
+هتتشوه شكل البكتيرياعشان هيك لازم نجففها عشان
+
+184
+00:13:10,250 --> 00:13:14,510
+مايصير اي lysis و نحافظ على شكلها يبقى عرفنا ليش
+
+185
+00:13:14,510 --> 00:13:18,950
+خطوة خطوة ال air drying to prevent lysis هلأ نرجع
+
+186
+00:13:18,950 --> 00:13:21,950
+على ال heat fixation قولنا هاي الخطوة كيف نعملها
+
+187
+00:13:21,950 --> 00:13:27,290
+نمرر ال slide تلت مرات على اللهب بسرعة طيب ليش انا
+
+188
+00:13:27,290 --> 00:13:30,930
+بدي اعمل هاي الخطوة من اسمها fixation to fix
+
+189
+00:13:30,930 --> 00:13:34,390
+بكتيريا بدي اثبت البكتيريا على ال slide لإن انا في
+
+190
+00:13:34,390 --> 00:13:38,730
+عندي خطوات لل standing بدي أصبع بدي أغسلبدي اغسل
+
+191
+00:13:38,730 --> 00:13:41,550
+اعمل washing فبالتالي ممكن البكتيريا خلال ال
+
+192
+00:13:41,550 --> 00:13:46,590
+washing انها تروح مع الميه عشان اضمن ان البكتيريا
+
+193
+00:13:46,590 --> 00:13:50,670
+ملتصقة على ال slide بعمل خطوة ال heat fixation طب
+
+194
+00:13:50,670 --> 00:13:54,170
+كيف البكتيريا بتلتصق على ال slide؟ بقولنا ال heat
+
+195
+00:13:54,170 --> 00:13:57,730
+الحرارة بتعمل partial melting of cell wall and
+
+196
+00:13:57,730 --> 00:14:03,030
+membrane of bacteria بتعمل تذويب بشكل بسيط لـالـ
+
+197
+00:14:03,030 --> 00:14:05,990
+cell wall والـ cell membrane هذا بيساعد إنه
+
+198
+00:14:05,990 --> 00:14:11,210
+البكتيريا تلتصق على الـ slide طيب، الهدف التالت من
+
+199
+00:14:11,210 --> 00:14:14,930
+هذه الخطوة تـ kill بكتيريا إذا متذكرين في الـ
+
+200
+00:14:14,930 --> 00:14:18,690
+bacterial morphology فيه دي طريقتين قلنا، الطريقة
+
+201
+00:14:18,690 --> 00:14:21,450
+الأولى living unstained الطريقة التانية killed
+
+202
+00:14:21,450 --> 00:14:25,110
+unstained organism في هذه الطريقة قلنا killed
+
+203
+00:14:25,110 --> 00:14:29,850
+بتكون البكتيريا ميت لأنه في خطوة من خطوات الصباغة
+
+204
+00:14:29,850 --> 00:14:35,110
+متمرر الـ slide على اللهبفبالتالي البكتيريا بتموت
+
+205
+00:14:35,110 --> 00:14:39,390
+في هذه الخطوة بيصير كل البكتيريا في هذه الخطوة
+
+206
+00:14:39,390 --> 00:14:43,270
+ماشي المقصود بالـ kill، القتل؟ مقصود إنه أنا بدي
+
+207
+00:14:43,270 --> 00:14:47,370
+أوقف حركة البكتيريا بدي أوقف الـ metabolism عشان
+
+208
+00:14:47,370 --> 00:14:52,190
+أقدر أحدد الـ morphology خلصنا من خطوة الـ heat
+
+209
+00:14:52,190 --> 00:14:57,050
+fixation هذا كله إنه فيه ملاحظة إنه أنا ما أعمل
+
+210
+00:14:57,050 --> 00:15:01,900
+overheat يعني بسرعة تلت مرات بسرعةلو خلّيتها كتير
+
+211
+00:15:01,900 --> 00:15:05,600
+على اللهب هذا هيسبب destroy لالبكتيريا انا مابدى
+
+212
+00:15:05,600 --> 00:15:10,460
+تتحلل البكتيريا مابدى تتدمر لأن انا فقط بدي احافظ
+
+213
+00:15:10,460 --> 00:15:15,760
+على شكلها حتى اني انا اقدر احدد ال morphology رابع
+
+214
+00:15:15,760 --> 00:15:20,640
+خطوة لستان تختلف حسب نوع الستان سواء كانت simple
+
+215
+00:15:20,640 --> 00:15:24,360
+سواء كانت differential سواء كانت special يبقى اول
+
+216
+00:15:24,360 --> 00:15:27,380
+خطوة و تاني خطوة و تالت خطوة هي نفسها هتكون بال
+
+217
+00:15:27,380 --> 00:15:31,660
+differential و ال specialلكن الاختلاف هيكون في رقم
+
+218
+00:15:31,660 --> 00:15:35,940
+أربعة في الـ Simple Stain اللي هو ما عملنا في هذا
+
+219
+00:15:35,940 --> 00:15:41,720
+اللقاء أول خطوة باستخدم Basic Stain وعرفنا ليش
+
+220
+00:15:41,720 --> 00:15:45,700
+لأنه الـ Basic Stain بتحمل شحنة موجبة والبكتيريا
+
+221
+00:15:45,700 --> 00:15:49,320
+شحنتها الكولية ثالثة واحنا قلنا الـ Simple Stain
+
+222
+00:15:49,320 --> 00:15:53,720
+اسمها Simple بسيطة بستخدم فقط الـ agent واحد اللي
+
+223
+00:15:53,720 --> 00:15:56,520
+هو الـ Basic Stain سواء استخدمت مثليني بلوك
+
+224
+00:15:56,520 --> 00:16:01,420
+كاربولفوكسين سفرانينأي basic stain بستخدم لمدة
+
+225
+00:16:01,420 --> 00:16:04,420
+معينة بعد ما اتخلص هذه المدة بعمل washing بال
+
+226
+00:16:04,420 --> 00:16:08,460
+distil water أو ال tap water بعدها في إيش إضافي
+
+227
+00:16:08,460 --> 00:16:11,340
+قولناه في الجدول ممكن أضيف ال mordant المثبت
+
+228
+00:16:11,340 --> 00:16:15,480
+الأيضائي بعدها بغسل نفس الشيء بعمل washing هذه
+
+229
+00:16:15,480 --> 00:16:18,040
+بالنسبة لخطوات ال symbol stain
+
+230
+00:16:22,100 --> 00:16:26,400
+هنمر على الـ «سلايدات» بشكل سريع لأن تقريبًا الـ
+
+231
+00:16:26,400 --> 00:16:31,200
+«سلايدتان» الأقبل لخّصوا كل الموضوع هنمعرف للـ
+
+232
+00:16:31,200 --> 00:16:34,700
+«سمير» «سمير» is a distribution of bacterial cell
+
+233
+00:16:34,700 --> 00:16:39,140
+on a slide for the purpose of viewing them under
+
+234
+00:16:39,140 --> 00:16:42,720
+the microscope يبقى هنفي عندي تعريف مهم جدًا اللي
+
+235
+00:16:42,720 --> 00:16:46,900
+هو «سمير» هو توزيع الخلايا البكتيرية على الـ
+
+236
+00:16:46,900 --> 00:16:50,340
+«سلايد» بحيث أني أنا أقدر أشوفها تحت الميكروسكوب
+
+237
+00:16:51,220 --> 00:16:54,660
+الطريقة أول شيء أسيبتيكالي إيش يعني أسيبتيكالي؟
+
+238
+00:16:54,660 --> 00:16:58,420
+أسيبتيك تكنيك هي طرق كل الطرق لمنع ال
+
+239
+00:16:58,420 --> 00:17:02,120
+contamination من أني أستخدم Sterile Loops أستخدم
+
+240
+00:17:02,120 --> 00:17:04,600
+Sterile Normal Saline الـ Normal Saline اللي أنا
+
+241
+00:17:04,600 --> 00:17:07,060
+بدي أستخدمه لازم يكون معقّم من عقمه في الـ
+
+242
+00:17:07,060 --> 00:17:10,740
+Autoclave قبل هذا كله لازم أني أنا أعقم الbench
+
+243
+00:17:10,740 --> 00:17:14,180
+اللي هشتغل عليه كذلك الشغل لازم يكون في منطقة
+
+244
+00:17:14,180 --> 00:17:17,420
+معقمة ممكن أني أنا أشتغل جانب اللهب أو بالـSeptic
+
+245
+00:17:17,420 --> 00:17:21,470
+Cabinet كل هذه اسمها أسيبتيك تكنيكaseptically a
+
+246
+00:17:21,470 --> 00:17:24,670
+small sample of the culture is spread over a slide
+
+247
+00:17:24,670 --> 00:17:26,890
+surface هذه الخطوة هي الـ smearing preparation
+
+248
+00:17:26,890 --> 00:17:32,690
+بوزع العينة على الـ slide بعدها بعرضها لـ بعمل air
+
+249
+00:17:32,690 --> 00:17:36,370
+drying حتى أمنع الـ lysis تالت خطوة اللي هي الـ
+
+250
+00:17:36,370 --> 00:17:41,190
+fixation وهذا كل السبب to help the cell adhere to
+
+251
+00:17:41,190 --> 00:17:45,910
+the slide surface أخر خطوة اللي هي الستانك يبقى
+
+252
+00:17:45,910 --> 00:17:48,230
+هاي كل الخطوات اللي ذكرناها
+
+253
+00:17:56,130 --> 00:18:01,230
+هن في بعض الخطوات مرسومة إنه استخدم اللي هو الـ
+
+254
+00:18:01,230 --> 00:18:04,930
+Broth Media أخد Bacterial Suspension أو Bacterial
+
+255
+00:18:04,930 --> 00:18:09,230
+Broth باستخدام اللوب حاول إنه يوزّي high الـ
+
+256
+00:18:09,230 --> 00:18:15,350
+Bacterial Suspension على الـ slide ويفرطهم هن drop
+
+257
+00:18:15,350 --> 00:18:18,870
+of normal saline أخد بكتيريا لوب full of bacterial
+
+258
+00:18:18,870 --> 00:18:22,650
+حاول يعمل mix و بعدها صار في عندي بعد الـ air
+
+259
+00:18:22,650 --> 00:18:26,940
+drying هيك بنحسهاالـ Normal Saline إذا ملاحظين هو
+
+260
+00:18:26,940 --> 00:18:31,820
+شفاف Transparent لما أنا أحط الكولوني بيصير شكله
+
+261
+00:18:31,820 --> 00:18:36,620
+زي Milky appearance زي شكل الحليب إذا متذكرين احنا
+
+262
+00:18:36,620 --> 00:18:39,580
+قلنا على الـ Thick سمير و Thin سمير و كيف أنا بدي
+
+263
+00:18:39,580 --> 00:18:44,040
+أحل هذه المشكلة بسمع حكي أنا كيف بدي أعرف إن والله
+
+264
+00:18:44,040 --> 00:18:47,640
+هل هو السمير Thick ولا Thin من خلال اللون خلال
+
+265
+00:18:47,640 --> 00:18:50,760
+شغلي إذا أنا لحظة اللون كتير غامق معناه إنه ممكن
+
+266
+00:18:50,760 --> 00:18:54,150
+احتمال يكون السمير عندي Thickلو كان فاتح اللون
+
+267
+00:18:54,150 --> 00:18:57,790
+معناه احتمال يكون عندي thin smear فبحاول ان انا
+
+268
+00:18:57,790 --> 00:19:01,670
+احل المشكلة لو انا بعد ما صبغته و يصار في عندي
+
+269
+00:19:01,670 --> 00:19:05,470
+drying ممكن ان انا اعيد الشغل مرة تانية و ان انا
+
+270
+00:19:05,470 --> 00:19:09,390
+احاول ان اضيف كمية اكتر من البكتيريا او من ال
+
+271
+00:19:09,390 --> 00:19:12,250
+normal saline حسب اذا كان thick او thin smear
+
+272
+00:19:15,970 --> 00:19:20,550
+in this exercise we will use simple stand to show
+
+273
+00:19:20,550 --> 00:19:24,930
+the general structure of some bacteria بقوللي ال
+
+274
+00:19:24,930 --> 00:19:27,470
+simple stand اللي اش انا بستخدمها اني انا اتعرف
+
+275
+00:19:27,470 --> 00:19:31,870
+على شكل البكتيريا usually a single basic stand
+
+276
+00:19:31,870 --> 00:19:35,410
+قولنا بنستخدم فقط reagent واحد من ال basic stand
+
+277
+00:19:35,410 --> 00:19:40,610
+في هذه الخطوةSimple Stain لا يقدر يقدر يقدر يقدر
+
+278
+00:19:40,610 --> 00:19:42,530
+يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر
+
+279
+00:19:42,530 --> 00:19:44,250
+يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر
+
+280
+00:19:44,250 --> 00:19:50,610
+يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر
+
+281
+00:19:50,610 --> 00:19:53,350
+يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر
+
+282
+00:19:53,350 --> 00:19:53,410
+يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر
+
+283
+00:19:53,410 --> 00:19:56,650
+يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر يقدر
+
+284
+00:20:07,320 --> 00:20:12,840
+They simply make the bacterial easier to see فقط
+
+285
+00:20:12,840 --> 00:20:18,660
+هي بتجعل البكتيريا سهل اني انا اشوفها هذه الخطوات
+
+286
+00:20:18,660 --> 00:20:24,000
+عند كل ان هو استخدم من البروث ميديا using
+
+287
+00:20:24,000 --> 00:20:28,520
+eniculating loop استخدم ال loop وأكيد طبعا لازم
+
+288
+00:20:28,520 --> 00:20:33,080
+ضروري يكون use sterile techniqueSmear the bacteria
+
+289
+00:20:33,080 --> 00:20:36,660
+بعمل بفرد البكتيريا من الـ Center حوالين و بعدين
+
+290
+00:20:36,660 --> 00:20:40,180
+بحاول أني أنا أروح على الأطراف if the bacterial
+
+291
+00:20:40,180 --> 00:20:43,480
+suspension is very thick واتكلمنا عن هذا الموضوع
+
+292
+00:20:43,480 --> 00:20:47,060
+إذا كان thick بضيف added a drop of water water
+
+293
+00:20:47,060 --> 00:20:50,540
+قصده فيها الـ normal saline تاني خطوة اللي هي الـ
+
+294
+00:20:50,540 --> 00:20:55,660
+air-drying كتب للسبب to prevent lysisالتالت خطوة
+
+295
+00:20:55,660 --> 00:21:00,560
+الـ Heat Fixation عن طريق تمريرها تمرير الـ slide
+
+296
+00:21:00,560 --> 00:21:04,620
+على اللها بتلت مرات بسرعة هذا بيعمل partial
+
+297
+00:21:04,620 --> 00:21:07,440
+melting of the cell wall and membrane of bacteria
+
+298
+00:21:07,440 --> 00:21:12,220
+and make them stick to the slide وتكلمنا عن هذا
+
+299
+00:21:12,220 --> 00:21:15,840
+الموضوع don't overheat لأنه بيسبب ليه destroy the
+
+300
+00:21:15,840 --> 00:21:22,290
+bacteriaأخر شئ بدي أعمل خطوة لستان الصبغة بدي أغمر
+
+301
+00:21:22,290 --> 00:21:27,150
+الاسمير بـ high الستان ببازكستان حسب المدة اللى
+
+302
+00:21:27,150 --> 00:21:31,110
+مكتوبة لكل نوع من أنواع الصبغات Carbolfoxin,
+
+303
+00:21:31,210 --> 00:21:34,570
+Methylenablo, نيجروزين إذا ملاحظين أنه الـ
+
+304
+00:21:34,570 --> 00:21:37,910
+Carbofoxin والميتلينيبلاو كانت من ضمن البازكستان
+
+305
+00:21:37,910 --> 00:21:41,870
+لكن النيجروزين كانت من ضمن الأسدكستان يبقى فيها
+
+306
+00:21:41,870 --> 00:21:46,330
+لأن في خطأ في ال slide من نصف نيجروزين لأنها تعتبر
+
+307
+00:21:46,330 --> 00:21:50,430
+أسدكستانما بستخدمها في الـ Simple Stain الـ Simple
+
+308
+00:21:50,430 --> 00:21:58,150
+Stain فقط بنستخدم Basic Stain بعد ما نغمر ال slide
+
+309
+00:21:58,150 --> 00:22:03,550
+بالستان وتنتهي المدة المحددة حسب كل صبغة بنضيف أو
+
+310
+00:22:03,550 --> 00:22:07,450
+بنعمل Washing بقولنا بتمسك ال slide بشكل موازي
+
+311
+00:22:07,450 --> 00:22:13,550
+يكون لالمجرى الميّه بعدها بتحاول انك انت تتنشفي ال
+
+312
+00:22:13,550 --> 00:22:18,210
+slide تمام Air-dryingبنشوف ال slide بعد ما تنشف
+
+313
+00:22:18,210 --> 00:22:21,930
+على ال microscope على عدسة ال air و ال oil lens
+
+314
+00:22:21,930 --> 00:22:28,230
+على عدسة 40 او او عشرة او ممكن نبدأ في عشرة اكس
+
+315
+00:22:28,230 --> 00:22:35,990
+اربعين وبعدين ننتقل لمية اكس هذه الخطوات نفس الشيء
+
+316
+00:22:35,990 --> 00:22:39,590
+قلنا لسمير ب preparation air drying heat fixation
+
+317
+00:22:39,590 --> 00:22:42,970
+وبعدين بدي انا اصبغ و اشوفها تحت ال microscope
+
+318
+00:22:48,490 --> 00:22:53,730
+هن بعض النتائج للـ Symbolist 10 أنا لو جبت سؤال
+
+319
+00:22:53,730 --> 00:22:58,470
+شريحة ويقولنا حد دي نتيجة الـ Symbolist 10 بدنا
+
+320
+00:22:58,470 --> 00:23:03,050
+ننتبه أنه بدنا شاب و Arrangement لأنه بيكون على
+
+321
+00:23:03,050 --> 00:23:07,270
+الشاب علاموي الـ Arrangement علامة هنشوف الشاب
+
+322
+00:23:07,270 --> 00:23:11,930
+الشاب هو إذا ملاحظين ككاي كراويةوالـ Arrangement
+
+323
+00:23:11,930 --> 00:23:16,090
+في عندي الخليتين ملتصقتين يبقى Diplococci من
+
+324
+00:23:16,090 --> 00:23:18,450
+الأمثلة على الـ Diplococci هنتعرف عليها أو
+
+325
+00:23:18,450 --> 00:23:24,010
+هتاخدوها إن نيسيريا هي تعتبر Diplococci البكتيريا
+
+326
+00:23:24,010 --> 00:23:28,750
+«هان» طبعا إذا ملاحظي إن هي عصوية بس ال «Lie»
+
+327
+00:23:28,750 --> 00:23:31,790
+بالنسبة لأ الـ Arrangement no Arrangement مافي
+
+328
+00:23:31,790 --> 00:23:38,000
+عندي أي شكل هي single بس ال «Lie»هذه البكتيريا هي
+
+329
+00:23:38,000 --> 00:23:41,960
+شكل كوكاي وبالنسبة للـarrangement إذا ملاحظين على
+
+330
+00:23:41,960 --> 00:23:47,000
+شكل إتشان يبقى streptococci في عندي البكتيريا
+
+331
+00:23:47,000 --> 00:23:50,700
+Acinetomyces اللي هي البكتيريا الخيطية هذه
+
+332
+00:23:50,700 --> 00:23:56,340
+البكتيريا هي طبعا شكلها بصلاي من خلال مشهد التشريح
+
+333
+00:23:56,340 --> 00:24:00,800
+أنا بقدر أشوفها فهيك بنكون خلصنا معمل ال symbol
+
+334
+00:24:00,800 --> 00:24:07,120
+standفيه هيكون مرفق مع الشرح الـ Video عن الجزء
+
+335
+00:24:07,120 --> 00:24:12,160
+العملي كيف الخطوات الـ Symbolistين مصورة وواضحة
+
+336
+00:24:12,160 --> 00:24:16,100
+وإن شاء الله تكون كل المعلومات في هذا الـ Chapter
+
+337
+00:24:16,100 --> 00:24:19,060
+واضحة والسلام عليكم ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/DBNaI7FWU-U_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/DBNaI7FWU-U_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..0594412aa9412fb1e5d29daa0da92c315f9ea689
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/DBNaI7FWU-U_raw.json
@@ -0,0 +1 @@
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انا اعيد الشغل مرة تانية و ان انا احاول ان اضيف كمية اكتر من البكتيريا او من ال normal saline حسب اذا كان thick او thin smear", "tokens": [1211, 2407, 25961, 6156, 9307, 5016, 13672, 11536, 20449, 8315, 3224, 1975, 33753, 2304, 6027, 7251, 30544, 18871, 16254, 5862, 899, 14881, 6156, 49628, 995, 12610, 16472, 1975, 8315, 1975, 5016, 1211, 9673, 8592, 28820, 3660, 45164, 1975, 8315, 39182, 19446, 20328, 3555, 17082, 47395, 4032, 7251, 9381, 9640, 8978, 18871, 16254, 22709, 3714, 43020, 16472, 1975, 8315, 1975, 3615, 25708, 25124, 17082, 1211, 3714, 25720, 6055, 7649, 10632, 4032, 16472, 1975, 8315, 1975, 5016, 995, 12610, 16472, 1975, 11242, 33911, 9122, 2304, 10632, 1975, 4117, 2655, 2288, 9154, 29739, 4117, 2655, 13546, 25528, 1975, 2407, 9154, 2423, 2710, 1845, 533, 11331, 35457, 1975, 15730, 25961, 5060, 1975, 2407, 5862, 899, 14881], "avg_logprob": -0.15555862198888729, "compression_ratio": 1.7991266375545851, "no_speech_prob": 1.6689300537109375e-06, "words": [{"start": 1132.83, 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"probability": 0.919189453125}, {"start": 1150.57, "end": 1150.85, "word": " كان", "probability": 0.9892578125}, {"start": 1150.85, "end": 1151.11, "word": " thick", "probability": 0.75537109375}, {"start": 1151.11, "end": 1151.37, "word": " او", "probability": 0.910888671875}, {"start": 1151.37, "end": 1151.67, "word": " thin", "probability": 0.87353515625}, {"start": 1151.67, "end": 1152.25, "word": " smear", "probability": 0.7462158203125}], "temperature": 1.0}, {"id": 43, "seek": 117675, "start": 1155.97, "end": 1176.75, "text": "in this exercise we will use simple stand to show the general structure of some bacteria بقوللي ال simple stand اللي اش انا بستخدمها اني انا اتعرف على شكل البكتيريا usually a single basic stand قولنا بنستخدم فقط reagent واحد من ال basic stand في هذه الخطوة", "tokens": [259, 341, 5380, 321, 486, 764, 2199, 1463, 281, 855, 264, 2674, 3877, 295, 512, 11763, 4724, 39648, 20292, 2423, 2199, 1463, 13672, 1829, 1975, 8592, 1975, 8315, 4724, 14851, 9778, 40448, 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+موسيقى
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/FS07l-gRrxk_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/FS07l-gRrxk_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..554f56eb7404cff8174b314447e5a163f31829ec
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/FS07l-gRrxk_postprocess.srt
@@ -0,0 +1,12 @@
+1
+00:00:19,620 --> 00:00:24,020
+السلام عليكم ورحمة الله وبركاته نستكمل معاكم سلسلة
+
+2
+00:00:24,020 --> 00:00:28,420
+Microbiology الجامعة الإسلامية فيديو اليوم بعنوان
+
+3
+00:00:28,420 --> 00:00:29,780
+Indus Virus 2
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/IM6QHSK9qWU.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/IM6QHSK9qWU.srt
new file mode 100644
index 0000000000000000000000000000000000000000..61d326174833b44c7aeee8975634b51e01418e9b
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/IM6QHSK9qWU.srt
@@ -0,0 +1,1660 @@
+1
+00:00:02,250 --> 00:00:05,570
+coagulase test هو الفحص الثاني اللي هنتعرف عليه في
+
+2
+00:00:05,570 --> 00:00:09,210
+هذه المحاضرة.  The coagulase test is one of the
+
+3
+00:00:09,210 --> 00:00:12,150
+biochemical tests. It is a very important test in the
+
+4
+00:00:12,150 --> 00:00:15,390
+microbiology. هو أحد الفحوصات الكيميائية المهمة في
+
+5
+00:00:15,390 --> 00:00:20,530
+microbiology عشان أشوف هل البكتيريا عندها أنزيم الـ
+
+6
+00:00:20,530 --> 00:00:23,370
+coagulase أم لا، أو هذا هيساعدني في الـ
+
+7
+00:00:23,370 --> 00:00:27,790
+identification. The coagulase test identifies whether
+
+8
+00:00:27,790 --> 00:00:33,030
+an organism produces the exoenzyme coagulase. الفحص
+
+9
+00:00:33,030 --> 00:00:37,150
+هيعرف لي البكتيريا التي تفرز أنزيم الـ Coagulase.
+
+10
+00:00:37,150 --> 00:00:42,170
+الـ Coagulase يعتبر Exo-enzyme، لكن الكاتلاز كان
+
+11
+00:00:42,170 --> 00:00:46,810
+Endo-enzyme، which causes the fibrinogen in blood
+
+12
+00:00:46,810 --> 00:00:50,530
+plasma to clot. واللي أشهر ما  يعمل أنزيم الـ
+
+13
+00:00:50,530 --> 00:00:54,830
+Coagulase، يحول الفيبرينوجين إلى فيبرين. الفيبرينوجين موجود في
+
+14
+00:00:54,830 --> 00:00:59,290
+البلازما، وهو بروتين موجود في blood plasma.  سيحول
+
+15
+00:00:59,290 --> 00:01:05,350
+الفيبرينوجين إلى فيبرين. الـ Fibrin هي الـ Clot، تمام،
+
+16
+00:01:05,350 --> 00:01:10,730
+طيب، ما هو الهدف من تكون الـ Clot؟ الجواب موجود في
+
+17
+00:01:10,730 --> 00:01:14,390
+الجملة التي بعدها. Organisms that produce coagulase
+
+18
+00:01:14,390 --> 00:01:18,510
+can form a protective barrier of fibrin around
+
+19
+00:01:18,510 --> 00:01:22,810
+themselves. بقول لي، الكائن الحي الذي يستطيع
+
+20
+00:01:22,810 --> 00:01:26,950
+أن ينتج أنزيم الـ coagulase، سيحول، قلنا، الـ Fibrinogen
+
+21
+00:01:26,950 --> 00:01:31,420
+إلى Fibrin، إلى Clot. هذه الـ clot ستكون  بمثابة protective
+
+22
+00:01:31,420 --> 00:01:38,080
+barrier، ستكون بمثابة واقي حول البكتيريا. around
+
+23
+00:01:38,080 --> 00:01:43,020
+themselves. البكتيريا، بقول لي، ستختبئ داخل هذه الـ clot
+
+24
+00:01:43,020 --> 00:01:48,020
+داخل هذه الفيبرين، الجلطة. بالتالي، making themselves
+
+25
+00:01:48,020 --> 00:01:51,320
+highly resistant to phagocytosis and other
+
+26
+00:01:51,320 --> 00:01:57,940
+immuno responses. بقول لي هذا الشيء سيجعل
+
+27
+00:01:57,940 --> 00:02:01,480
+الـ  Immunosystem، جهاز المناعة، غير قادر على التعامل معها.
+
+28
+00:02:01,480 --> 00:02:04,540
+بالتالي ستكون highly resistant للبكتيريا
+
+29
+00:02:04,540 --> 00:02:09,140
+طبعاً، البكتيريا  من  الـ Immunoresponse
+
+30
+00:02:09,140 --> 00:02:13,200
+لأ، الـ Immunoresponse بشكل عام أيضاً، and some other
+
+31
+00:02:13,200 --> 00:02:17,940
+antimicrobial agents.  لو مضاد حيوي يريد القضاء عليها، لن
+
+32
+00:02:17,940 --> 00:02:22,960
+يجدها، ستكون مختبئة داخل الـ clot. يبقى
+
+33
+00:02:22,960 --> 00:02:26,780
+الـ Clot ستكون بمثابة protective barrier حول
+
+34
+00:02:26,780 --> 00:02:30,460
+البكتيريا، ستكون بمثابة حامي حول البكتيريا، بالتالي
+
+35
+00:02:30,460 --> 00:02:34,240
+جهاز المناعة لن يتمكن من التعرف عليها، ولا المضادات
+
+36
+00:02:34,240 --> 00:02:39,300
+الحيوية. significance
+
+37
+00:02:39,300 --> 00:02:43,840
+قلنا إنه يجب أن نعرف لكل فحص ما هي أهميته،
+
+38
+00:02:43,840 --> 00:02:48,380
+significance، ما هي أهمية الفحص، بين من ومن سيميز هذا الفحص
+
+39
+00:02:49,950 --> 00:02:53,250
+سنفرق بين، في هذه الـ slide، قلنا أهمية
+
+40
+00:02:53,250 --> 00:02:57,110
+الفحص. لكن الـ slide التي قبلها كان protective
+
+41
+00:02:57,110 --> 00:03:01,110
+barrier. هذا، ما هو هدف هذا الإنزيم؟ أو ماذا سيفعل
+
+42
+00:03:01,110 --> 00:03:07,210
+هذا الإنزيم؟ يبقى، هنا، فائدة الإنزيم. لكن هنا، أهمية الفحص.
+
+43
+00:03:07,210 --> 00:03:11,550
+The coagulase test is used to differentiate the
+
+44
+00:03:11,550 --> 00:03:15,130
+potentially pathogenic species Staphylococcus
+
+45
+00:03:15,130 --> 00:03:19,440
+aureus from other gram-positive cocci, the usually
+
+46
+00:03:19,440 --> 00:03:24,720
+non-pathogenic species. قلنا إنه جرام
+
+47
+00:03:24,720 --> 00:03:29,580
+موجب.  أيضا،  لدينا  Staphylococcus micrococcus,
+
+48
+00:03:29,580 --> 00:03:34,160
+macrococcus and streptococcus. فرقنا بينهم بفحص الكاتلاز.
+
+49
+00:03:34,160 --> 00:03:38,460
+كان الكاتلاز سالب في الـ streptococcus لكن الكاتلاز موجب في
+
+50
+00:03:38,460 --> 00:03:44,610
+Staphylococcus micrococcus, macrococcus. طيب، كيف نفرق بين
+
+51
+00:03:44,610 --> 00:03:47,870
+الـ staphylococcus و الـ micrococcus و الـ micrococcus؟  هناك فحص سنتعرف عليه
+
+52
+00:03:47,870 --> 00:03:51,710
+في المحاضرة القادمة، وهو الـ Oxidase.  الـ staphylococcus
+
+53
+00:03:51,710 --> 00:03:55,350
+هي Oxidase سالبة. لكن الـ micrococcus و الـ micrococcus
+
+54
+00:03:55,350 --> 00:03:58,850
+Oxidase موجبة.  قمنا بعمل هذا الفحص وكانت النتيجة Oxidase
+
+55
+00:03:58,850 --> 00:04:04,490
+سلبية، يبقى البكتيريا هي Staphylococcus. طيب،
+
+56
+00:04:04,490 --> 00:04:09,660
+الـ Staphylococcus aureus تعتبر Genus. لدي أنواع من
+
+57
+00:04:09,660 --> 00:04:12,700
+الـ Staphylococcus. لدي Staphylococcus aureus، لدي
+
+58
+00:04:12,700 --> 00:04:15,360
+Staphylococcus epidermidis، لدي Staphylococcus
+
+59
+00:04:15,360 --> 00:04:19,240
+saprophyticus. كيف أفرق بين الـ Staphylococcus
+
+60
+00:04:19,240 --> 00:04:23,440
+species؟ بقول لي من خلال فحص الـ Coagulase.
+
+61
+00:04:23,440 --> 00:04:28,120
+الـ Staphylococcus aureus تعتبر Coagulase موجبة،
+
+62
+00:04:28,120 --> 00:04:32,240
+موجبة. لكن الـ non-pathogenic species، وهي
+
+63
+00:04:32,240 --> 00:04:35,220
+Staphylococcus epidermidis و Staphylococcus saprophyticus، هي
+
+64
+00:04:35,220 --> 00:04:39,290
+Coagulase سالبة. الـ Staphylococcus aureus
+
+65
+00:04:39,290 --> 00:04:42,310
+potentially pathogenic in humans and animals. هي
+
+66
+00:04:42,310 --> 00:04:44,990
+تعتبر ممرضة. لكن الـ Staphylococcus
+
+67
+00:04:44,990 --> 00:04:49,150
+epidermidis و الـ saprophyticus تعتبر opportunistic
+
+68
+00:04:49,150 --> 00:04:53,710
+انتهازية، أي أنها تستغل ضعف جهاز المناعة، أسميها
+
+69
+00:04:53,710 --> 00:04:58,130
+nosocomial، تسبب nosocomial infection أو الـ
+
+70
+00:04:58,130 --> 00:05:01,150
+hospital-acquired infection أو الـ healthcare
+
+71
+00:05:01,150 --> 00:05:05,730
+associated infection. هذه نفس المسميات، نفس الـ
+
+72
+00:05:05,730 --> 00:05:10,300
+nosocomial infection. يبقى فحص الـ Coagulase أهميته
+
+73
+00:05:10,300 --> 00:05:14,160
+أنه يميز الـ Staphylococcus aureus، التي تكون
+
+74
+00:05:14,160 --> 00:05:17,140
+Coagulase موجبة، عن باقي أنواع الـ species من
+
+75
+00:05:17,140 --> 00:05:20,220
+الـ Staphylococcus التي تكون سالبة. الـ Staphylococcus
+
+76
+00:05:20,220 --> 00:05:28,020
+epidermidis و الـ Staphylococcus saprophyticus. نلاحظ
+
+77
+00:05:28,020 --> 00:05:32,140
+في الصورة، الـ Staphylococcus، قلنا إنها عنقودية، فنلاحظ
+
+78
+00:05:32,140 --> 00:05:36,470
+أنها على شكل عنقود.  وبالطبع هي Gram
+
+79
+00:05:36,470 --> 00:05:39,830
+-positive. جرام موجبة، تأخذ اللون البنفسجي
+
+80
+00:05:39,830 --> 00:05:46,050
+واللون البنفسجي.  أنواع الـ Coagulase.  يقول لي، هناك
+
+81
+00:05:46,050 --> 00:05:50,650
+أنواع لـ Coagulase. Coagulase enzyme، يقول لي، يوجد
+
+82
+00:05:50,650 --> 00:05:55,150
+بشكلين، قد يكون Bound Coagulase، وقد يكون
+
+83
+00:05:55,150 --> 00:06:00,660
+Free Coagulase. الـ Bound Coagulase يسمى أيضاً
+
+84
+00:06:00,660 --> 00:06:04,460
+clumping factor. يبقى الـ Bound له اسم آخر، وهو
+
+85
+00:06:04,460 --> 00:06:11,480
+الـ Clumping Factor. It is attached to the bacterial cell
+
+86
+00:06:11,480 --> 00:06:15,640
+wall and reacts directly with the fibrinogen in
+
+87
+00:06:15,640 --> 00:06:19,420
+plasma. بقول لي، الـ Bound، من اسمه، تفرزه الخلية
+
+88
+00:06:19,420 --> 00:06:25,200
+البكتيرية ويخرج للخارج لكنه يظل مرتبطاً بجدار الخلية.
+
+89
+00:06:25,200 --> 00:06:28,920
+It is attached to the bacterial cell wall. يظل مرتبطاً
+
+90
+00:06:28,920 --> 00:06:31,940
+بجدار الخلية. قلنا إنه له اسم آخر، وهو
+
+91
+00:06:31,940 --> 00:06:35,760
+clumping factor and reacts directly with the
+
+92
+00:06:35,760 --> 00:06:39,280
+fibrinogen in plasma.  يقول لي، يحدث التفاعل بشكل
+
+93
+00:06:39,280 --> 00:06:43,560
+مباشر، وهذا ما يميزه عن النوع الآخر.
+
+94
+00:06:43,560 --> 00:06:48,070
+التفاعل يكون مباشر مع الفيبرينوجين. الـ
+
+95
+00:06:48,070 --> 00:06:51,270
+Substrate الذي سيعمل عليه الإنزيم، سواء Free
+
+96
+00:06:51,270 --> 00:06:56,350
+أو Bound، هو الفيبرينوجين الموجود في
+
+97
+00:06:56,350 --> 00:07:01,410
+البلازما. سنستخدم، سنفعل مستعمرات
+
+98
+00:07:01,410 --> 00:07:04,790
+البكتيريا مع البلازما. البلازما التي تحتوي على
+
+99
+00:07:04,790 --> 00:07:08,590
+الـ Fibrinogen. يقول لي إن التفاعل يحدث بشكل مباشر.
+
+100
+00:07:08,590 --> 00:07:12,210
+سيتفاعل مع الـ Fibrinogen. الـ Substrate هو
+
+101
+00:07:12,210 --> 00:07:15,250
+الـ Fibrinogen. سيعمل عليه الـ Coagulase بشكل
+
+102
+00:07:15,250 --> 00:07:20,290
+مباشر. سيتحول الـ Fibrinogen بواسطة أنزيم الـ
+
+103
+00:07:20,290 --> 00:07:24,310
+Coagulase إلى Fibrin، إلى Clot. يبقى لدي خطوة واحدة
+
+104
+00:07:24,310 --> 00:07:28,890
+واحدة. في one step.  تحول الـ Fibrinogen بواسطة أنزيم
+
+105
+00:07:28,890 --> 00:07:33,330
+الـ Coagulase إلى Fibrin في خطوة واحدة. بالتالي،
+
+106
+00:07:33,330 --> 00:07:37,330
+الـ Bound Coagulase يكون بشكل Rapid، بشكل سريع.
+
+107
+00:07:37,330 --> 00:07:42,350
+فبالتالي،  أكتشفه باستخدام  الـ Slide Method فقط. يبقى،
+
+108
+00:07:42,350 --> 00:07:45,250
+إذا أردنا مقارنة الـ Bound Coagulase، اسمه Clumping
+
+109
+00:07:45,250 --> 00:07:49,880
+Factor. يحدث بشكل مباشر، direct، المعادلة
+
+110
+00:07:49,880 --> 00:07:54,700
+الفيبرينوجين يتحول إلى فيبرين إلى Clot في one step، الشيء
+
+111
+00:07:54,700 --> 00:07:58,960
+يكون سريعاً، الوقت يكون سريعاً. فبالتالي، فقط
+
+112
+00:07:58,960 --> 00:08:03,860
+أكتشفه بالـ slide method. لكن الـ free coagulase
+
+113
+00:08:03,860 --> 00:08:08,720
+هو إنزيم خارج الخلية، extracellular enzyme، تفرزه الخلية ويخرج
+
+114
+00:08:08,720 --> 00:08:13,100
+خارج الخلية.  لا يلتصق بجدار الخلية البكتيرية مثل
+
+115
+00:08:13,100 --> 00:08:17,200
+الـ bound coagulase. فبالتالي، يُعتبر
+
+116
+00:08:17,200 --> 00:08:21,380
+Extracellular enzyme released from the cell that
+
+117
+00:08:21,380 --> 00:08:26,200
+reacts with a plasma component called coagulase
+
+118
+00:08:26,200 --> 00:08:29,400
+reacting factor. بقول لي، التفاعل يحدث بشكل
+
+119
+00:08:29,400 --> 00:08:33,440
+غير مباشر، indirectly، على عكس الـ bound. كيف
+
+120
+00:08:33,440 --> 00:08:37,900
+يحدث بشكل غير مباشر؟ بقول لي، قبل حدوث التفاعل،
+
+121
+00:08:37,900 --> 00:08:42,240
+الـ free coagulase يتفاعل مع مكوّن من مكونات
+
+122
+00:08:42,240 --> 00:08:46,850
+البلازما، اسمه coagulase reacting factor. هذا اختصاره
+
+123
+00:08:46,850 --> 00:08:52,030
+الـ Coagulase يتفاعل مع الـ Coagulase Reacting
+
+124
+00:08:52,030 --> 00:08:57,350
+Factor، ويحول البروثرومبين إلى ثرومبين. سيتحول
+
+125
+00:08:57,350 --> 00:09:03,170
+إلى البروثرومبين إلى ثرومبين، وبعدها الثرومبين هو الذي سيحول
+
+126
+00:09:03,170 --> 00:09:07,710
+الفيبرينوجين إلى فيبرين إلى فيبرين إلى Clot. يبقى لدي
+
+127
+00:09:07,710 --> 00:09:14,050
+two steps.  عندما يتفاعل الـ Coagulase مع الـ Coagulase
+
+128
+00:09:14,050 --> 00:09:18,410
+Reacting Factor، يحول البروثرومبين إلى ثرومبين. بعد ذلك،
+
+129
+00:09:18,410 --> 00:09:21,970
+الثرومبين هو الذي يحول الفيبرينوجين إلى فيبرين. يبقى في
+
+130
+00:09:21,970 --> 00:09:26,670
+عندي two steps. سيستغرق وقتاً طويلاً. فبالتالي، الـ Free
+
+131
+00:09:26,670 --> 00:09:33,070
+Coagulase أكتشفه باستخدام  Lithium Method.  Lithium
+
+132
+00:09:33,070 --> 00:09:38,310
+Method  أكتشف به الـ Free Coagulase. هذا بالنسبة لـ
+
+133
+00:09:38,520 --> 00:09:42,240
+الأنواع أو أشكال الـ coagulase.  لدي Bound ولدي
+
+134
+00:09:42,240 --> 00:09:46,740
+Free Coagulase. بقول لي، التفاعل
+
+135
+00:09:46,740 --> 00:09:53,020
+النتيجة هي نفسها  في  الـ bound coagulase، لكن يتحول إلى
+
+136
+00:09:53,020 --> 00:09:56,540
+بروثرومبين، ثم يتحول إلى فيبرينوجين، ويحدث
+
+137
+00:09:56,540 --> 00:09:57,540
+جلطة.
+
+138
+00:10:06,720 --> 00:10:10,280
+هنا، مقارنة بين الـ bound coagulase و الـ free
+
+139
+00:10:10,280 --> 00:10:16,100
+coagulase. الـ Bound Coagulase، له اسم آخر، قلنا،
+
+140
+00:10:16,100 --> 00:10:18,400
+هو Clumping Factor. الـ Free Coagulase
+
+141
+00:10:18,400 --> 00:10:23,460
+Extracellular Coagulase، لأن قلنا يخرج خارج الخلية
+
+142
+00:10:23,460 --> 00:10:27,620
+ولا يلتصق بجدار الخلية البكتيرية.  التفاعل
+
+143
+00:10:27,620 --> 00:10:31,580
+مباشر، لأنه يحول الفيبرينوجين إلى فيبرين
+
+144
+00:10:31,580 --> 00:10:35,160
+بواسطة أنزيم الـ Bound Coagulase. لكن الـ Free
+
+145
+00:10:35,160 --> 00:10:40,190
+Coagulase غير مباشر، لأنه يتفاعل مع الـ coagulase
+
+146
+00:10:40,190 --> 00:10:43,730
+reacting factor.  في الـ free coagulase، بالتالي يحول
+
+147
+00:10:43,730 --> 00:10:46,510
+البروثرومبين إلى ثرومبين، ثم الثرومبين هو الذي
+
+148
+00:10:46,510 --> 00:10:51,510
+يحول الفيبرينوجين إلى فيبرين. بما أنه مباشر، خطوة
+
+149
+00:10:51,510 --> 00:10:56,050
+واحدة، بالتالي سيستغرق وقتاً أقل. few times. لكن الـ free
+
+150
+00:10:56,050 --> 00:10:59,350
+coagulase خطوتان، بالتالي سيستغرق وقتاً أطول. tag
+
+151
+00:10:59,350 --> 00:11:04,210
+time, work area. بقول لي، المساحة محدودة، لأن قلنا
+
+152
+00:11:04,210 --> 00:11:09,030
+هو bound coagulase فقط، سيكون على السطح، على جدار الخلية
+
+153
+00:11:09,030 --> 00:11:13,390
+موجود، فبالتالي المساحة التي يمكن أن يعمل عليها
+
+154
+00:11:13,390 --> 00:11:17,550
+محدودة. لكن الـ free coagulase واسعة، broad،
+
+155
+00:11:17,550 --> 00:11:23,370
+لأنه يخرج خارج الخلية، فيمكنه العمل في أي مكان. heat
+
+156
+00:11:23,370 --> 00:11:28,030
+tolerance. الـ bound coagulase مستقر حرارياً، heat stable، يتحمل
+
+157
+00:11:28,030 --> 00:11:32,190
+درجة الحرارة. لكن الـ free coagulase غير مستقر، labile،
+
+158
+00:11:32,190 --> 00:11:37,530
+يتأثر بدرجة الحرارة. الـ bound coagulase أكتشفه
+
+159
+00:11:37,530 --> 00:11:41,390
+بـ slide and tube method. لكن الـ free coagulase، الـ
+
+160
+00:11:41,390 --> 00:11:45,270
+tube method. لأن الـ free coagulase يستغرق وقتاً
+
+161
+00:11:45,270 --> 00:11:50,250
+طويلاً. فبالتالي، الـ tube method سأفعل incubation
+
+162
+00:11:50,250 --> 00:11:54,330
+أربع ساعات. لكن الـ slide بشكل مباشر، فأكتشف الـ
+
+163
+00:11:54,330 --> 00:12:00,020
+bound بـ slide and tube method. الفحص يعتبر الـ
+
+164
+00:12:00,020 --> 00:12:02,920
+bound coagulase  فحصاً أولياً، screening، لكن الـ free
+
+165
+00:12:02,920 --> 00:12:09,060
+coagulase تأكيدي، confirmatory، لأن الـ bound هو الذي أكتشفه في
+
+166
+00:12:09,060 --> 00:12:13,420
+الـ slide and tube method، لكن الـ free في الـ tube
+
+167
+00:12:13,420 --> 00:12:17,280
+method. يبقى الـ bound coagulase يعتبر فحصاً أولياً، لكن
+
+168
+00:12:17,280 --> 00:12:19,840
+الـ free coagulase يعتبر تأكيدياً.
+
+169
+00:12:28,350 --> 00:12:31,510
+كيف يمكنني أن أكتشف، أو كيف يمكنني عمل فحص الـ
+
+170
+00:12:31,510 --> 00:12:34,290
+Coagulase؟  قلنا، طريقتان، وهما  الـ tube
+
+171
+00:12:34,290 --> 00:12:39,390
+method ستكشف لي عن وجود الـ presence of bound or
+
+172
+00:12:39,390 --> 00:12:43,250
+free. لكن الـ slide method فقط ستكشف لي عن الـ bound
+
+173
+00:12:43,250 --> 00:12:47,050
+coagulase. فبالتالي، الـ tube method تعتبر
+
+174
+00:12:47,050 --> 00:12:50,370
+تأكيدية، confirmatory، لكن الـ slide method تعتبر فحصاً أولياً، screening
+
+1
+
+223
+00:17:24,110 --> 00:17:27,950
+لي أنت أول شيء بتحط drop من rabbit plasma و
+
+224
+00:17:27,950 --> 00:17:31,550
+بعدين بتحط touch of bacteria بقولنا ممكن أن نحط
+
+225
+00:17:31,800 --> 00:17:35,460
+أو لازم نحط distilled water أو normal saline جنب
+
+226
+00:17:35,460 --> 00:17:40,340
+القطرة في البلازما هذا هيكون as a negative control
+
+227
+00:17:40,340 --> 00:17:56,420
+كيف يعني أنا جبت ال slide قلنا
+
+228
+00:17:56,420 --> 00:17:58,600
+أضيف drop من القطرة في البلازما
+
+229
+00:18:05,830 --> 00:18:12,910
+هنا وهنا كمان drop من distilled water
+
+230
+00:18:12,910 --> 00:18:18,210
+أو
+
+231
+00:18:18,210 --> 00:18:19,850
+normal saline
+
+232
+00:18:28,620 --> 00:18:30,960
+الـ drop من الـ rabbit plasma والـ drop من الـ
+
+233
+00:18:30,960 --> 00:18:33,220
+distilled water أو الـ normal saline بعدين بجيب
+
+234
+00:18:33,220 --> 00:18:35,900
+البكتيريا اللي أنا بدي أكشف هل موجودة عندها
+
+235
+00:18:35,900 --> 00:18:43,220
+الأنزيم ولا لأ و بحط هنا بكتيريا يعني
+
+236
+00:18:43,220 --> 00:18:50,860
+في كل drop هنا بكتيريا هحط وهنا
+
+237
+00:18:50,860 --> 00:18:53,200
+نفس البكتيريا اللي أنا بدي أكشف عليها
+
+238
+00:19:18,370 --> 00:19:22,050
+يبقى بنحط rabbit plasma drop من الـ distilled water
+
+239
+00:19:22,050 --> 00:19:25,150
+بجيب البكتيريا اللي بدي أكشف عنها هل عندها الانزيم
+
+240
+00:19:25,150 --> 00:19:28,690
+ولا لأ بحطها drop و بعمل mix مع rabbit plasma و
+
+241
+00:19:28,690 --> 00:19:31,730
+بحطها كولوني من البكتيريا اللي أنا بدي أكشف عندها
+
+242
+00:19:31,730 --> 00:19:36,090
+الأنزيم نفس اللي حطيتها هنا و بعمل mix طيب ليش هذه
+
+243
+00:19:36,090 --> 00:19:38,710
+الفكرة قولنا هذا ال distilled water أو ال normal
+
+244
+00:19:38,710 --> 00:19:41,470
+saline مع البكتيريا اللي بدي أكشف عنها تعتبر as a
+
+245
+00:19:41,470 --> 00:19:46,870
+negative control هلأ لو البكتيريا عندها ال enzyme
+
+246
+00:19:46,870 --> 00:19:51,850
+لو البكتيريا عندها ال enzyme أكيد هتشتغل على ال
+
+247
+00:19:51,850 --> 00:19:54,570
+substrate اللي هي الفيبرينوجين اللي موجود في
+
+248
+00:19:54,570 --> 00:20:00,490
+البلازما وهتعطيني هتحول الفيبرينوجين لفيبرين هيصير
+
+249
+00:20:00,490 --> 00:20:03,970
+في عندي agglutination هيكون في عندي clot يبقى
+
+250
+00:20:03,970 --> 00:20:09,770
+بتكون النتيجة لو positive هي هتكون positive لو
+
+251
+00:20:09,770 --> 00:20:14,230
+عندها ال enzyme أكيد هي هتكون positive لكن لو أنا
+
+252
+00:20:14,230 --> 00:20:18,360
+عملت نفس البكتيريا اللي عندها الانزيم عملت mix مع
+
+253
+00:20:18,360 --> 00:20:22,980
+distilled water أكيد هتكون النتيجة هنا negative مش هيكون
+
+254
+00:20:22,980 --> 00:20:28,980
+فيه عندي أي clot أي حبيبات ليش لأنه ما فيه عند
+
+255
+00:20:28,980 --> 00:20:31,760
+ال substrate اللي هيشتغل عليها الانزيم هنا فيه عند
+
+256
+00:20:31,760 --> 00:20:34,060
+البلازما موجود فيها الفيبرينوجين لكن ال normal
+
+257
+00:20:34,060 --> 00:20:37,080
+saline أو الـ distilled water مش موجود فيه ال substrate اللي
+
+258
+00:20:37,080 --> 00:20:39,720
+هو الفيبرينوجين اللي هيشتغل عليها الانزيم يعني حتى
+
+259
+00:20:39,720 --> 00:20:44,030
+لو كان الانزيم موجود في البكتيريا أكيد في ال
+
+260
+00:20:44,030 --> 00:20:47,450
+negative control هتكون negative ما هيكون في عندي
+
+261
+00:20:47,450 --> 00:20:51,130
+agglutination طيب هذه في الحالة كانت هي positive
+
+262
+00:20:51,130 --> 00:20:55,750
+result خلينا نكتب هنا وكان في عندي هنا positive
+
+263
+00:20:55,750 --> 00:20:59,830
+result لو كان عندها ال enzyme
+
+264
+00:21:11,410 --> 00:21:15,190
+طيب لو كانت ما عندها البكتيريا لو جبت بكتيريا
+
+265
+00:21:15,190 --> 00:21:18,990
+ثانية هذه البكتيريا هي negative لل coagulase ما
+
+266
+00:21:18,990 --> 00:21:23,470
+عندها الانزيم ال coagulase هعمل mix مع ال rabbit
+
+267
+00:21:23,470 --> 00:21:27,410
+plasma فأكيد هتكون النتيجة هنا مش مش موجود الانزيم
+
+268
+00:21:27,410 --> 00:21:31,250
+لكن موجود ال substrate لكن مش هيصير في عندي تفاعل
+
+269
+00:21:31,250 --> 00:21:37,630
+هتكون negative لو حطيتها مع ال normal saline
+
+270
+00:21:37,630 --> 00:21:41,770
+لا يوجد فيه أنزيمات أو سبسترات فهو لن يكون فيه
+
+271
+00:21:41,770 --> 00:21:47,850
+agglutination فهو negative يبقى هنا في حال كانت negative
+
+272
+00:21:47,850 --> 00:21:48,210
+result
+
+273
+00:22:05,780 --> 00:22:09,360
+يبقى في كلتا الحالتين الـ negative control لازم
+
+274
+00:22:09,360 --> 00:22:14,180
+يكون negative لأنه ال normal saline مش
+
+275
+00:22:14,180 --> 00:22:18,040
+موجود فيه ال substrate اللي هي ال fibrinogen
+
+276
+00:22:18,040 --> 00:22:23,240
+اللي هيشتغل عليها ال enzyme طيب لو كانت النتيجة
+
+277
+00:22:23,240 --> 00:22:28,440
+عملت جبت بكتيريا وحطيتها مع البلازما وطلعت
+
+278
+00:22:28,440 --> 00:22:32,960
+positive وحطيتها
+
+279
+00:22:32,960 --> 00:22:41,650
+مع ال normal saline وبرضه طلعت positive إحنا
+
+280
+00:22:41,650 --> 00:22:45,070
+قلنا إنه ال negative control لازم يكون negative
+
+281
+00:22:45,070 --> 00:22:48,490
+لأنه ال normal saline ما عندي fibrinogen مش
+
+282
+00:22:48,490 --> 00:22:51,350
+موجود ال substrate اللي هيشتغل عليه ال enzyme طيب
+
+283
+00:22:51,350 --> 00:22:54,550
+من وين صار عندي ال agglutination في ال negative
+
+284
+00:22:54,550 --> 00:22:58,350
+control واللي هنا بيكون في عندي مشكلة قلنا ممنوع
+
+285
+00:22:58,350 --> 00:23:01,350
+تطلع ال negative control positive لأنه ما في
+
+286
+00:23:01,350 --> 00:23:03,990
+substrate الفيبرينوجين اللي هيشتغل عليه ال enzyme
+
+287
+00:23:04,540 --> 00:23:08,900
+طب إيش السبب اللي خلّى صار عندي فيه agglutination
+
+288
+00:23:08,900 --> 00:23:13,320
+بقول السبب إن البكتيريا بتعمل autoagglutination
+
+289
+00:23:13,320 --> 00:23:19,460
+يعني بشكل ذاتي بدون وجود enzyme autoagglutination
+
+290
+00:23:33,940 --> 00:23:39,480
+في عندي تفسيرها إن في عندي autoagglutination مش
+
+291
+00:23:39,480 --> 00:23:43,860
+ناتج عن وجود إنزيم الكواغولايز هي بتفرز clumping
+
+292
+00:23:43,860 --> 00:23:50,000
+factor فبيصير في عندي agglutination يبقى بالخلاصة
+
+293
+00:23:50,000 --> 00:23:53,020
+أنا مش عارفة في هذه العينة لو كانت في الـ... لو
+
+294
+00:23:53,020 --> 00:23:55,600
+كانت أنا خلطت بـ irreversible plasma مع البكتيريا
+
+295
+00:23:55,600 --> 00:23:59,290
+في هذه الحالة أنا مش عارفة هل البكتيريا عندها إنزيم
+
+296
+00:23:59,290 --> 00:24:03,190
+الـ coagulase وعملت agglutination ولا ما عندها
+
+297
+00:24:03,190 --> 00:24:07,970
+وكان ال agglutination اللي ظهر في ال sample من ال
+
+298
+00:24:07,970 --> 00:24:13,140
+autoagglutination بقول لي الحل في هذه الحالة إن أنا عشان
+
+299
+00:24:13,140 --> 00:24:15,780
+أعرف في هذه الحالة لو طلع positive في positive
+
+300
+00:24:15,780 --> 00:24:20,060
+لازم أتأكد بالـ tube method قلنا الـ tube method
+
+301
+00:24:20,060 --> 00:24:24,440
+هي confirmatory تأكيدية لل slides screening فلازم
+
+302
+00:24:24,440 --> 00:24:27,160
+أنا أعمل ال tube method اللي هنتعرف عليها كمان
+
+303
+00:24:27,160 --> 00:24:33,140
+شوية ال tube method هي اللي هتعرفني هل هو auto
+
+304
+00:24:33,140 --> 00:24:36,800
+agglutination ولا لأ لو طلعت positive يبقى هي مش
+
+305
+00:24:36,800 --> 00:24:40,660
+autoagglutination لو طلعت negative يبقى هو الـ
+
+306
+00:24:40,660 --> 00:24:44,460
+issue اللي صار autoagglutination والنتيجة هي
+
+307
+00:24:44,460 --> 00:24:48,800
+negative مش positive يبقى هذا بالنسبة للـ slide
+
+308
+00:24:48,800 --> 00:24:49,280
+method
+
+309
+00:25:06,200 --> 00:25:10,400
+طبعاً بنعمل بعد ما إحنا عملنا اللي هو drop of
+
+310
+00:25:10,400 --> 00:25:12,900
+rabbit plasma مع touch of بكتيريا وحطينا negative
+
+311
+00:25:12,900 --> 00:25:18,580
+control بقول لي بتعملي mix بال rabbit
+
+312
+00:25:18,580 --> 00:25:22,540
+plasma أو كمان بال water أو ال normal saline
+
+313
+00:25:22,540 --> 00:25:27,180
+يعني اللي هو negative control بعدين بشوف النتيجة
+
+314
+00:25:27,180 --> 00:25:31,540
+بقول لي
+
+315
+00:25:31,540 --> 00:25:35,470
+observe for clumping in the coagulase plasma and
+
+316
+00:25:35,470 --> 00:25:39,430
+homogenous suspension in the control at 20 to 30
+
+317
+00:25:39,430 --> 00:25:43,350
+seconds بقول لي بلاحظ ال clumping اللي موجود فيه ال
+
+318
+00:25:43,350 --> 00:25:46,690
+coagulase plasma ال drop اللي حطيتها من البلازما و
+
+319
+00:25:46,690 --> 00:25:50,230
+ال drop اللي حطيته في ال control هنا في عندي
+
+320
+00:25:50,230 --> 00:25:53,750
+ملاحظات clumping that will not mix uniformly into
+
+321
+00:25:53,750 --> 00:25:57,510
+coagulase plasma indicate a positive test بقول لي
+
+322
+00:25:57,970 --> 00:26:01,290
+الـ positive result بيقول في العين بيكون clumping
+
+323
+00:26:01,290 --> 00:26:06,230
+تجمع من اللي فيه بيكون في العين حبيبات clots agglutination
+
+324
+00:26:06,230 --> 00:26:09,990
+زي فصائل الدم لكن بالنسبة للـ negative result
+
+325
+00:26:09,990 --> 00:26:12,770
+بيكون في العين uniform suspension أو smooth
+
+326
+00:26:12,770 --> 00:26:16,230
+suspension بيكون ال suspension كثير ناعم ما فيه أي
+
+327
+00:26:16,230 --> 00:26:20,250
+clot تمام هذه النتيجة بتكون negative result يبقى
+
+328
+00:26:20,250 --> 00:26:23,090
+الـ positive result بيكون في العين clumping تجمع لكن
+
+329
+00:26:23,090 --> 00:26:27,390
+الـ negative uniform suspension clumping in both
+
+330
+00:26:27,390 --> 00:26:30,750
+tests indicate that the organism is autoagglutination
+
+331
+00:26:30,750 --> 00:26:34,670
+that is unsuitable for the slide coagulase
+
+332
+00:26:34,670 --> 00:26:40,250
+test قلنا لو أنا عملت الفحص وطلع في ال sample و
+
+333
+00:26:40,250 --> 00:26:43,470
+في ال control كان عندي positive positive هذا يدل
+
+334
+00:26:43,470 --> 00:26:46,870
+على أنه عندي autoagglutination ال
+
+335
+00:26:46,870 --> 00:26:50,250
+autoagglutination مش مناسب لل slide coagulase test
+
+336
+00:26:50,250 --> 00:26:53,850
+لازم أكد الفحص بال tube method لأنه هي ال
+
+337
+00:26:53,850 --> 00:26:58,000
+confirmatory when the autoagglutination is observed
+
+338
+00:26:58,000 --> 00:27:00,280
+واللي بقول لنا الـ tube method قلنا لازم أني أنا
+
+339
+00:27:00,280 --> 00:27:04,520
+أشتغلها بدل من ال slide method لأن قلنا ال tube
+
+340
+00:27:04,520 --> 00:27:07,180
+method هو ال confirmatory
+
+341
+00:27:16,790 --> 00:27:19,790
+procedure of the tube method خلصنا من ال slide
+
+342
+00:27:19,790 --> 00:27:22,470
+method وعرفنا كيف بنشتغلها وقلنا هي screening
+
+343
+00:27:22,470 --> 00:27:26,890
+method هلق هنتكلم على ال confirmatory method اللي
+
+344
+00:27:26,890 --> 00:27:31,450
+هو ال tube method اللي بيكشف عن ال free and bound
+
+345
+00:27:31,450 --> 00:27:35,210
+عشان هي أنا قلت يعتبر confirmatory لأنه بيكشف عن
+
+346
+00:27:35,210 --> 00:27:40,230
+ال free والـ bound ما بيكشف عن في حال كان فيه auto
+
+347
+00:27:40,230 --> 00:27:44,870
+agglutination برضه الـ tube method أنا بستخدمها
+
+348
+00:27:44,870 --> 00:27:47,330
+بدل من ال slide method فبالتالي هو يعتبر
+
+349
+00:27:47,330 --> 00:27:51,970
+confirmatory لكن ال slide فقط بتكشف لي عن ال bound
+
+350
+00:27:51,970 --> 00:27:57,560
+ال bound coagulase أي فحص كيميائي أول جملة بقول
+
+351
+00:27:57,560 --> 00:28:00,680
+using a culture that is less than 24 hours old
+
+352
+00:28:00,680 --> 00:28:04,000
+بقول لنا أي فحص كيميائي لازم ال culture تكون fresh
+
+353
+00:28:04,000 --> 00:28:09,580
+لأن ال enzyme activity بتقل و بقول بتأخذ من مزرعة
+
+354
+00:28:09,580 --> 00:28:14,470
+أو من culture a non-inhibitory agar plate non-
+
+355
+00:28:14,470 --> 00:28:19,030
+inhibitory يعني إنه يأخذ الكولوني من أجار non-
+
+356
+00:28:19,030 --> 00:28:22,670
+selective media أفضل أني أنا أشتغل من general
+
+357
+00:28:22,670 --> 00:28:25,970
+media لأن الـ selective media فيها inhibitor هذه
+
+358
+00:28:25,970 --> 00:28:30,830
+الـ inhibitor ممكن تؤثر على الانزيم أشهر الطريقة
+
+359
+00:28:30,830 --> 00:28:34,910
+بقول لنا إنه بتجيب 2 ل 4 كولونيات من البكتيريا اللي
+
+360
+00:28:34,910 --> 00:28:40,720
+بدك تكشف عندها الانزيم موجود أو لأ بتجيبيهم هاي و
+
+361
+00:28:40,720 --> 00:28:44,520
+بتحطيهم في 1 ml of rabbit plasma وبتعملي لهم
+
+362
+00:28:44,520 --> 00:28:49,800
+incubation لمدة أربع ساعات على درجة حرارة 37 و
+
+363
+00:28:49,800 --> 00:28:55,420
+ثلاثين خلال الأربع ساعات كل شوية بطلع و بحرك بلاحظ
+
+364
+00:28:55,420 --> 00:28:58,360
+إذا تكونت جلطة أو لأ كيف تكونت جلطة فيه
+
+365
+00:28:58,360 --> 00:29:04,320
+بيكون clot زي حبيبات في السائل أو ممكن نلاحظ إن
+
+366
+00:29:04,320 --> 00:29:08,160
+السائل بدأ يجمد فبالتالي هاي النتيجة ال positive
+
+367
+00:29:08,870 --> 00:29:31,830
+يبقى الـ tube method الـ
+
+368
+00:29:31,830 --> 00:29:34,330
+tube method بجيب اثنين
+
+369
+00:29:59,820 --> 00:30:10,280
+of bacteria زائد 1
+
+370
+00:30:10,280 --> 00:30:13,640
+ml of rabbit plasma
+
+371
+00:30:28,250 --> 00:30:44,630
+بعملهم incubation على
+
+372
+00:30:44,630 --> 00:30:51,770
+درجة حرارة 37 درجة سليزية لمدة 4 hours
+
+373
+00:31:01,560 --> 00:31:06,300
+في الـ incubator 37 درجة سليزية لمدة 4 ساعات وبشوف
+
+374
+00:31:06,300 --> 00:31:11,500
+النتيجة قلنا خلال 4 ساعات بحاول أني أنا أشيك على
+
+375
+00:31:11,500 --> 00:31:15,840
+ال tube بشوف إذا في عندي تكون clot ولا لأ بشوف
+
+376
+00:31:15,840 --> 00:31:20,000
+إذا السائل جمد أو لأ هلق لو كانت النتيجة positive
+
+377
+00:31:20,000 --> 00:31:27,220
+كان في عندي clot
+
+378
+00:31:31,890 --> 00:31:36,870
+أو لاحظت إن السائل جمد فتمام يبقى بحكي إنه هي
+
+379
+00:31:36,870 --> 00:31:41,190
+النتيجة positive في عندي إنزيم الـ coagulase لكن
+
+380
+00:31:41,190 --> 00:31:51,210
+لو ما كان في عندي أي clot وكانت  الـ  clot السائل
+
+381
+00:31:51,210 --> 00:31:55,310
+ظل زي ما هو بقول لي النتيجة ما بسجلها negative
+
+382
+00:31:55,310 --> 00:32:00,830
+تمام ما بنسجل النتيجة negative بقول لنا بروح أنا
+
+383
+00:32:00,830 --> 00:32:06,070
+بعمل incubation بكمل ال incubation لـ 24 ساعة إحنا
+
+384
+00:32:06,070 --> 00:32:09,390
+عملنا 4 ساعات يبقى ضل في عندي 20 ساعة بعمل
+
+385
+00:32:09,390 --> 00:32:13,570
+incubation لمدة 20 ساعة لكن على ال room
+
+386
+00:32:13,570 --> 00:32:17,210
+temperature يبقى لو ما كان عندي تكون ال clot
+
+387
+00:32:17,210 --> 00:32:21,030
+ما بحكي negative بعملها incubation هذه النتيجة يبقى
+
+388
+00:32:21,030 --> 00:32:24,390
+بعملها incubation
+
+389
+00:32:34,180 --> 00:32:40,660
+at room temperature لمدة
+
+390
+00:32:40,660 --> 00:32:46,120
+20 ساعة و
+
+391
+00:32:46,120 --> 00:32:50,940
+اللي هو of a total 24 hours إحنا عملنا 4 ساعات يبقى
+
+392
+00:32:50,940 --> 00:32:55,240
+بدي عشان أكمل للـ 24 بحطها 24 ساعة لكن على room
+
+393
+00:32:55,240 --> 00:32:59,680
+temperature بعد بعد ما تخلص الـ 20 ساعة يعني الـ
+
+394
+00:32:59,680 --> 00:33:04,980
+total 24 ساعة لو كان في عندي no clot يبقى خلاص
+
+395
+00:33:04,980 --> 00:33:08,800
+بعتمد النتيجة negative لكن لو اتغير وصار في عندي
+
+396
+00:33:08,800 --> 00:33:12,980
+clot يبقى هي positive يبقى هنا في المرحلة هاي أنا
+
+397
+00:33:12,980 --> 00:33:17,840
+بحكي إنه لو تكون clot يبقى هي positive
+
+398
+00:33:26,710 --> 00:33:33,330
+لكن هنا خلاص بعد 24 ساعة بتكون كافية إني أنا أحكي
+
+399
+00:33:33,330 --> 00:33:40,410
+إنه هي negative no clot أو السائل ما جمد يبقى
+
+400
+00:33:40,410 --> 00:33:45,030
+بعتبر إنه النتيجة هنا negative يبقى بعد 24 ساعة
+
+401
+00:33:45,030 --> 00:33:49,370
+بأكد النتيجة ال negative هذا بالنسبة لليوتيوب
+
+402
+00:33:49,370 --> 00:33:52,890
+method هيك الطريقة هيك الشغل لليوتيوب method
+
+403
+00:34:00,080 --> 00:34:03,320
+بقول لنا negative test بقول لنا لو الفحص كان negative
+
+404
+00:34:03,320 --> 00:34:06,320
+بعد أربع ساعات بعمله على ال room temperature زي ما
+
+405
+00:34:06,320 --> 00:
+
+445
+00:36:52,150 --> 00:36:57,900
+لا Free ولا Bound،  التكنيكية الـ slide agglutination
+
+446
+00:36:57,900 --> 00:37:04,180
+قد تؤدي إلى نتيجة false positive، بقول لي أن الـ slide
+
+447
+00:37:04,180 --> 00:37:08,620
+method ممكن تعطيني نتيجة false positive. لماذا؟ لأن
+
+448
+00:37:08,620 --> 00:37:12,520
+بعض
+
+449
+00:37:12,520 --> 00:37:16,580
+الأنواع من البكتيريا تنتج نتيجة false positive، الـ
+
+450
+00:37:16,580 --> 00:37:19,460
+clumping factor نفسه هو معناه أنها بتعمل auto
+
+451
+00:37:19,460 --> 00:37:23,580
+agglutination، يبقى بتعطيني نتيجة false positive في
+
+452
+00:37:23,580 --> 00:37:29,110
+الـ slide. عشان هيك لازم أنا أعيد النتيجة بالـ tube
+
+453
+00:37:29,110 --> 00:37:32,330
+method. قلنا الـ tube method هو يعتبر confirmatory
+
+454
+00:37:32,330 --> 00:37:36,310
+ولا لازم كمان أنا أعمل negative control. لو طلعت معي
+
+455
+00:37:36,310 --> 00:37:39,270
+positive، معناه أن في عندي... لو طلع negative control
+
+456
+00:37:39,270 --> 00:37:42,750
+positive، معناه في عندي autoagglutination، لازم أعيد
+
+457
+00:37:42,750 --> 00:37:46,720
+الفحص بالـ tube method، الـ confirmatory. لو بالـ tube
+
+458
+00:37:46,720 --> 00:37:50,840
+method طلع positive، يبقى هي بتكون coagulase
+
+459
+00:37:50,840 --> 00:37:54,400
+positive. لكن لو طلع negative بتكون هي coagulase
+
+460
+00:37:54,400 --> 00:37:57,680
+negative. الـ agglutination اللي ظهر هو نتيجة الـ
+
+461
+00:37:57,680 --> 00:38:01,800
+autoagglutination فقط. spontaneous agglutination
+
+462
+00:38:01,800 --> 00:38:04,240
+may occur when rough cultures are used. بقول لي
+
+463
+00:38:04,240 --> 00:38:06,640
+spontaneous agglutination هو نفس الـ
+
+464
+00:38:06,640 --> 00:38:10,800
+autoagglutination. بلاحظه خصوصًا لو أخدت البكتيريا
+
+465
+00:38:10,800 --> 00:38:15,500
+من... لو كانت الـ colony non-capsulated، يعني معناه إنه
+
+466
+00:38:15,500 --> 00:38:21,160
+non-capsulated. بكتيريا، ليش أنا بلاحظ من الـ auto
+
+467
+00:38:21,160 --> 00:38:24,020
+agglutination بيصير في الـ non-capsulated bacteria؟
+
+468
+00:38:24,020 --> 00:38:29,140
+احنا قلنا الكبسولة تعتبر أو تستخدم كـ protection
+
+469
+00:38:29,140 --> 00:38:33,570
+from الـ Phagocytosis، والـ Coagulase Enzyme نفس
+
+470
+00:38:33,570 --> 00:38:37,070
+الفكرة، قلنا بيحول الفيبرينوجين لفيبرين، الفيبرين
+
+471
+00:38:37,070 --> 00:38:40,990
+يخثّر، البكتيريا بتتخبّى جوا هذا الفيبرين، فبالتالي
+
+472
+00:38:40,990 --> 00:38:44,750
+بتحميها من الـ Phagocytosis. يبقى لو ما عندها
+
+473
+00:38:44,750 --> 00:38:47,970
+capsule، أكيد هتلجأ للـ Coagulase، أو لو ما عندها
+
+474
+00:38:47,970 --> 00:38:51,910
+Coagulase، أكيد هتلجأ للـ autoagglutination عشان
+
+475
+00:38:51,910 --> 00:38:55,610
+يصير في عندي... عشان تحميها من الـ Phagocytosis.
+
+476
+00:38:55,610 --> 00:38:59,480
+فبنلاحظ أن الـ spontaneous agglutination بيكون فيه
+
+477
+00:38:59,480 --> 00:39:03,580
+الـ non-capsulated bacteria. بقول لي آخر شيء أنه الـ
+
+478
+00:39:03,580 --> 00:39:07,140
+tube method أفضل من الـ slide method. قلنا لأنه الـ
+
+479
+00:39:07,140 --> 00:39:10,820
+tube method بيكشف عن الـ free و الـ bound، لكن الـ
+
+480
+00:39:10,820 --> 00:39:15,280
+slide بيكشف عن الـ bound فقط. الـ slide بيصير فيه
+
+481
+00:39:15,280 --> 00:39:18,340
+تجفيف و agglutination، لازم أني أنا أعمل الـ tube
+
+482
+00:39:18,340 --> 00:39:23,310
+method عشان أتأكد. The slide test should be read very
+
+483
+00:39:23,310 --> 00:39:26,050
+quickly. بقول لي لو أنت اشتغلت بـ slide method لازم
+
+484
+00:39:26,050 --> 00:39:29,630
+تقرأيها بسرعة. false positive result ممكن أنه يصير.
+
+485
+00:39:29,630 --> 00:39:34,170
+The test should then be performed with organisms detected
+
+486
+00:39:34,170 --> 00:39:37,590
+from high salt media such as mannitol salt agar.
+
+487
+00:39:37,590 --> 00:39:42,510
+احنا قلنا إنه أي فحص كيميائي لازم أن أنا اشتغله من
+
+488
+00:39:42,510 --> 00:39:45,390
+general media. الـ mannitol salt agar تعتبر selective
+
+489
+00:39:45,390 --> 00:39:48,590
+media، فيها inhibitor اللي هو الـ salt. بقول الـ salt
+
+490
+00:39:48,590 --> 00:39:53,550
+ممكن أنه يؤثر على الإنزيمات، فبالتالي تعطيني false
+
+491
+00:39:53,550 --> 00:39:56,950
+positive result. هذه النتيجة، إذا ما نلاحظ الـ
+
+492
+00:39:56,950 --> 00:39:59,030
+Staphylococcus aureus، قلنا هي positive للـ
+
+493
+00:39:59,030 --> 00:40:01,650
+Coagulase، في عندي حبيبات، في عندي clots، لكن
+
+494
+00:40:01,650 --> 00:40:05,090
+Staphylococcus epidermidis هي negative للـ
+
+495
+00:40:05,090 --> 00:40:07,850
+Coagulase، بنلاحظ أنه في عندي smooth suspension،
+
+496
+00:40:07,850 --> 00:40:10,990
+uniform، ما في عندي أي حبيبات. هذا بالنسبة للـ slide
+
+497
+00:40:10,990 --> 00:40:14,490
+method. لكن للـ tube method، الـ coagulase positive
+
+498
+00:40:14,490 --> 00:40:18,620
+بنلاحظ أن الـ plasma جمدت، لكن الـ coagulase
+
+499
+00:40:18,620 --> 00:40:22,200
+negative، أنه ما في عندي clot، والبلازما ضلت سائل.
+
+500
+00:40:22,200 --> 00:40:26,480
+حيث إننا خلصنا الـ Coagulase Test و Catalase Test،
+
+501
+00:40:26,480 --> 00:40:31,180
+وحيث إننا خلصنا الـ chapter لليوم، والسلام عليكم
+
+502
+00:40:31,180 --> 00:40:32,560
+ورحمة الله وبركاته
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/IM6QHSK9qWU_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/IM6QHSK9qWU_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..351fbf3317164ba52dbc0e02571962b2690cbfa2
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/IM6QHSK9qWU_raw.json
@@ -0,0 +1 @@
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22.27, "end": 22.89, "word": " هيساعدني", "probability": 0.90478515625}, {"start": 22.89, "end": 23.15, "word": " في", "probability": 0.98583984375}, {"start": 23.15, "end": 23.37, "word": " ال", "probability": 0.90283203125}, {"start": 23.37, "end": 24.15, "word": " identification", "probability": 0.94140625}, {"start": 24.15, "end": 25.37, "word": " the", "probability": 0.7470703125}, {"start": 25.37, "end": 26.15, "word": " coagulase", "probability": 0.986572265625}, {"start": 26.15, "end": 26.49, "word": " test", "probability": 0.9345703125}, {"start": 26.49, "end": 27.39, "word": " identify", "probability": 0.7900390625}, {"start": 27.39, "end": 27.79, "word": " whether", "probability": 0.853515625}, {"start": 27.79, "end": 28.27, "word": " an", "probability": 0.86328125}, {"start": 28.27, "end": 28.81, "word": " organism", "probability": 0.97412109375}, {"start": 28.81, "end": 29.61, "word": " produce", "probability": 0.640625}, {"start": 29.61, "end": 30.01, "word": " the", "probability": 0.90283203125}, {"start": 30.01, "end": 31.01, "word": " exoenzyme", "probability": 0.88232421875}, {"start": 31.01, "end": 31.85, "word": " coagulase", "probability": 0.95556640625}], "temperature": 1.0}, {"id": 2, "seek": 6135, "start": 32.43, "end": 61.35, "text": "الفحص هيعرف لي البكتيريا اللي هتفرز أنزيم الـ Coagulase الـ Coagulase يعتبر Exo-enzyme لكن الكتلاز كان Endo-enzyme which causes the fibrinogen of blood plasma to clot واللي أشهر بيعمل أنزيم الـ Coagulase بيحول الفيبرينوجين الفيبرينوجين موجود في البلازمة هو بروتيين موجود في blood plasma هيحول الفيبرينوجين لفيبرين", "tokens": [6027, 5172, 5016, 9381, 39896, 3615, 28480, 32239, 29739, 4117, 2655, 13546, 25528, 13672, 1829, 8032, 2655, 5172, 2288, 11622, 14739, 11622, 32640, 2423, 39184, 3066, 559, 425, 651, 2423, 39184, 3066, 559, 425, 651, 7251, 34268, 26890, 2111, 78, 12, 268, 1229, 1398, 44381, 33251, 2655, 1211, 31377, 25961, 6967, 78, 12, 268, 1229, 1398, 597, 7700, 264, 283, 6414, 259, 8799, 295, 3390, 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"word": " Endo", "probability": 0.640869140625}, {"start": 42.79, "end": 43.49, "word": "-enzyme", "probability": 0.8663330078125}, {"start": 43.49, "end": 44.45, "word": " which", "probability": 0.27685546875}, {"start": 44.45, "end": 45.11, "word": " causes", "probability": 0.76708984375}, {"start": 45.11, "end": 45.49, "word": " the", "probability": 0.86572265625}, {"start": 45.49, "end": 46.21, "word": " fibrinogen", "probability": 0.904541015625}, {"start": 46.21, "end": 46.39, "word": " of", "probability": 0.8935546875}, {"start": 46.39, "end": 46.81, "word": " blood", "probability": 0.8603515625}, {"start": 46.81, "end": 47.37, "word": " plasma", "probability": 0.9501953125}, {"start": 47.37, "end": 47.67, "word": " to", "probability": 0.89013671875}, {"start": 47.67, "end": 47.99, "word": " clot", "probability": 0.88818359375}, {"start": 47.99, "end": 49.27, "word": " واللي", "probability": 0.8019205729166666}, {"start": 49.27, "end": 49.51, "word": " أشهر", "probability": 0.6751708984375}, {"start": 49.51, "end": 49.93, "word": " بيعمل", "probability": 0.9385986328125}, {"start": 49.93, "end": 50.29, "word": " أنزيم", "probability": 0.9593098958333334}, {"start": 50.29, "end": 50.53, "word": " الـ", "probability": 0.9345703125}, {"start": 50.53, "end": 51.21, "word": " Coagulase", "probability": 0.963134765625}, {"start": 51.21, "end": 51.95, "word": " بيحول", "probability": 0.9404296875}, {"start": 51.95, "end": 53.09, "word": " الفيبرينوجين", "probability": 0.784423828125}, {"start": 53.09, "end": 54.09, "word": " الفيبرينوجين", "probability": 0.9622395833333334}, {"start": 54.09, "end": 54.67, "word": " موجود", "probability": 0.9112955729166666}, {"start": 54.67, "end": 54.83, "word": " في", "probability": 0.9482421875}, {"start": 54.83, "end": 55.35, "word": " البلازمة", "probability": 0.906982421875}, {"start": 55.35, "end": 55.55, "word": " هو", "probability": 0.923828125}, {"start": 55.55, "end": 56.15, "word": " بروتيين", "probability": 0.8194580078125}, {"start": 56.15, "end": 56.69, "word": " موجود", "probability": 0.97314453125}, {"start": 56.69, "end": 56.91, "word": " في", "probability": 0.98486328125}, {"start": 56.91, "end": 57.43, "word": " blood", "probability": 0.8759765625}, {"start": 57.43, "end": 57.99, "word": " plasma", "probability": 0.96728515625}, {"start": 57.99, "end": 59.29, "word": " هيحول", "probability": 0.89892578125}, {"start": 59.29, "end": 60.39, "word": " الفيبرينوجين", "probability": 0.947998046875}, {"start": 60.39, "end": 61.35, "word": " لفيبرين", "probability": 0.82421875}], "temperature": 1.0}, {"id": 3, "seek": 8845, "start": 61.79, "end": 88.45, "text": "الـ Fibrin هي الـ Clot تمام، طيب، شو الهدف لما تتكون لـ Clot؟ الجواب موجود في الجملة اللي بعدها organism that produce coagulase can form a protective barrier of fibrin around themselves، بقولّي ال organism اللي بتقدر إنها تنتج أنزيم الـ coagulase هتحول قلنا الـ Fibrinogen لـ Fibrin، لـ Clot", "tokens": [6027, 39184, 479, 6414, 259, 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{"start": 70.57, "end": 70.73, "word": " في", "probability": 0.96728515625}, {"start": 70.73, "end": 71.07, "word": " الجملة", "probability": 0.93310546875}, {"start": 71.07, "end": 71.21, "word": " اللي", "probability": 0.9140625}, {"start": 71.21, "end": 71.65, "word": " بعدها", "probability": 0.7288818359375}, {"start": 71.65, "end": 72.19, "word": " organism", "probability": 0.283935546875}, {"start": 72.19, "end": 72.61, "word": " that", "probability": 0.6962890625}, {"start": 72.61, "end": 73.35, "word": " produce", "probability": 0.509765625}, {"start": 73.35, "end": 74.39, "word": " coagulase", "probability": 0.9110107421875}, {"start": 74.39, "end": 74.89, "word": " can", "probability": 0.80810546875}, {"start": 74.89, "end": 75.47, "word": " form", "probability": 0.9560546875}, {"start": 75.47, "end": 75.91, "word": " a", "probability": 0.90869140625}, {"start": 75.91, "end": 76.35, "word": " protective", "probability": 0.79248046875}, {"start": 76.35, "end": 77.07, "word": " barrier", "probability": 0.9404296875}, {"start": 77.07, "end": 77.37, "word": " of", "probability": 0.97265625}, {"start": 77.37, "end": 78.01, "word": " fibrin", "probability": 0.859375}, {"start": 78.01, "end": 78.51, "word": " around", "probability": 0.9521484375}, {"start": 78.51, "end": 79.43, "word": " themselves،", "probability": 0.516845703125}, {"start": 79.43, "end": 79.87, "word": " بقولّي", "probability": 0.590545654296875}, {"start": 79.87, "end": 80.59, "word": " ال", "probability": 0.60986328125}, {"start": 80.59, "end": 81.43, "word": " organism", "probability": 0.83251953125}, {"start": 81.43, "end": 81.91, "word": " اللي", "probability": 0.961669921875}, {"start": 81.91, "end": 82.53, "word": " بتقدر", "probability": 0.9715169270833334}, {"start": 82.53, "end": 82.81, "word": " إنها", "probability": 0.614501953125}, {"start": 82.81, "end": 83.27, "word": " تنتج", "probability": 0.9900716145833334}, {"start": 83.27, "end": 83.71, "word": " أنزيم", "probability": 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themselves البكتيريا بقولي هتتخبى جوا هذه ال clot جوا هذه الفيبرين الجلطة بالتالي making themselves highly resistant to phagocytosis other immunoresponse بقولي هذا ال .. الاشي", "tokens": [3224, 24192, 2423, 39184, 48587, 8032, 2655, 30544, 30767, 1829, 16314, 13357, 8032, 2655, 30544, 30767, 1829, 36764, 38436, 11331, 2407, 6027, 9957, 29739, 4117, 2655, 13546, 25528, 926, 2969, 29739, 4117, 2655, 13546, 25528, 4724, 39648, 1829, 8032, 2655, 2655, 9778, 3555, 7578, 10874, 14407, 29538, 2423, 48587, 10874, 14407, 29538, 27188, 1829, 26890, 9957, 25724, 1211, 9566, 3660, 4724, 6027, 2655, 6027, 1829, 1455, 2969, 5405, 20383, 281, 903, 559, 905, 4328, 8211, 661, 13154, 2706, 79, 3739, 4724, 39648, 1829, 23758, 2423, 4386, 2423, 33599, 1829], "avg_logprob": -0.23524305472771326, "compression_ratio": 1.6883720930232557, "no_speech_prob": 1.7881393432617188e-07, "words": [{"start": 89.1, "end": 89.52, "word": "هذه", "probability": 0.776611328125}, {"start": 89.52, "end": 89.64, "word": " 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0.458740234375}, {"start": 99.16, "end": 100.08, "word": " البكتيريا", "probability": 0.98515625}, {"start": 100.08, "end": 100.46, "word": " بقولي", "probability": 0.7882486979166666}, {"start": 100.46, "end": 101.32, "word": " هتتخبى", "probability": 0.9261067708333334}, {"start": 101.32, "end": 101.66, "word": " جوا", "probability": 0.8271484375}, {"start": 101.66, "end": 102.1, "word": " هذه", "probability": 0.7216796875}, {"start": 102.1, "end": 102.56, "word": " ال", "probability": 0.97314453125}, {"start": 102.56, "end": 103.02, "word": " clot", "probability": 0.40283203125}, {"start": 103.02, "end": 103.5, "word": " جوا", "probability": 0.870849609375}, {"start": 103.5, "end": 103.8, "word": " هذه", "probability": 0.80908203125}, {"start": 103.8, "end": 104.4, "word": " الفيبرين", "probability": 0.73834228515625}, {"start": 104.4, "end": 105.22, "word": " الجلطة", "probability": 0.967529296875}, {"start": 105.22, "end": 106.7, "word": " بالتالي", "probability": 0.819970703125}, 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202.78, "word": " احنا", "probability": 0.7670084635416666}, {"start": 202.78, "end": 203.24, "word": " قلنا", "probability": 0.8956705729166666}, {"start": 203.24, "end": 204.2, "word": " انه", "probability": 0.752685546875}, {"start": 204.2, "end": 204.72, "word": " لجرام", "probability": 0.7828369140625}, {"start": 204.72, "end": 205.2, "word": " positive", "probability": 0.7685546875}, {"start": 205.2, "end": 205.84, "word": " ككاى", "probability": 0.62615966796875}, {"start": 205.84, "end": 206.04, "word": " في", "probability": 0.8330078125}, {"start": 206.04, "end": 206.42, "word": " عندي", "probability": 0.6212158203125}, {"start": 206.42, "end": 207.14, "word": " استفيله", "probability": 0.742919921875}, {"start": 207.14, "end": 207.78, "word": " ككاى", "probability": 0.9405517578125}, {"start": 207.78, "end": 208.64, "word": " micro", "probability": 0.61279296875}, {"start": 208.64, "end": 209.58, "word": " ككاى", "probability": 0.9400634765625}, {"start": 209.58, "end": 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والمايكرو؟ في فحص سنتعرف عليه في المعمل اللي جاي اللي هو الـ Oxidase الاستافيلو كوكاي هي Oxidase negative لكن المايكرو والمايكرو Oxidase positive فعملت هذا الفحص وطلعت Oxidase negative يبقى البكتيريا الاستافيلو كوكاي طيب الاستافيلو كوكاي تعتبر Genus", "tokens": [4117, 33911, 8717, 5172, 2288, 4587, 49374, 42963, 14851, 31845, 26895, 2407, 16070, 15042, 1829, 4117, 32887, 16070, 15042, 1829, 4117, 32887, 22807, 8978, 6156, 5016, 9381, 8608, 29399, 3615, 28480, 47356, 8978, 9673, 25957, 1211, 13672, 1829, 10874, 47302, 13672, 1829, 31439, 2423, 39184, 16489, 327, 651, 42963, 14851, 31845, 26895, 2407, 9122, 2407, 4117, 47302, 39896, 16489, 327, 651, 3671, 44381, 2423, 15042, 1829, 4117, 32887, 16070, 15042, 1829, 4117, 32887, 16489, 327, 651, 3353, 6156, 25957, 1211, 2655, 23758, 27188, 5016, 9381, 4032, 9566, 1211, 34268, 16489, 327, 651, 3671, 7251, 3555, 4587, 7578, 29739, 4117, 2655, 13546, 25528, 42963, 14851, 31845, 26895, 2407, 9122, 2407, 4117, 47302, 23032, 1829, 3555, 42963, 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"probability": 0.85009765625}, {"start": 239.25, "end": 239.75, "word": " يبقى", "probability": 0.976806640625}, {"start": 239.75, "end": 240.91, "word": " البكتيريا", "probability": 0.9396484375}, {"start": 240.91, "end": 242.73, "word": " الاستافيلو", "probability": 0.88486328125}, {"start": 242.73, "end": 243.71, "word": " كوكاي", "probability": 0.8902587890625}, {"start": 243.71, "end": 244.49, "word": " طيب", "probability": 0.7906087239583334}, {"start": 244.49, "end": 245.41, "word": " الاستافيلو", "probability": 0.965234375}, {"start": 245.41, "end": 245.97, "word": " كوكاي", "probability": 0.916259765625}, {"start": 245.97, "end": 246.73, "word": " تعتبر", "probability": 0.9459635416666666}, {"start": 246.73, "end": 247.45, "word": " Genus", "probability": 0.6944580078125}], "temperature": 1.0}, {"id": 10, "seek": 27676, "start": 247.84, "end": 276.76, "text": "في عندي أنا species من الاستفيلو كوكاي في عندي استفيلو كوكاس aureus في عندي استفيلو كوكاس epidermidis في عندي استفيلو كوكاس saprophyticas كيف بدأ أفرق بين الاستفيلو كوكاس species بقولني من خلال فحص الـ Coagulase الاستفيلو كوكاس aureus هي تعتبر positive Coagulase positive لكن الـ non pathogenic species اللي هي الاستفيلو كوكاس epidermidis و الـ saprophyticas هي Coagulase negative", "tokens": [41185, 18871, 16254, 41850, 6172, 9154, 42963, 14851, 5172, 26895, 2407, 9122, 2407, 4117, 47302, 8978, 18871, 16254, 44713, 5172, 26895, 2407, 9122, 2407, 4117, 32277, 257, 540, 301, 8978, 18871, 16254, 44713, 5172, 26895, 2407, 9122, 2407, 4117, 32277, 13510, 966, 40920, 8978, 18871, 16254, 44713, 5172, 26895, 2407, 9122, 2407, 4117, 32277, 18985, 11741, 43658, 296, 9122, 33911, 47525, 10721, 5551, 5172, 2288, 4587, 49374, 42963, 14851, 5172, 26895, 2407, 9122, 2407, 4117, 32277, 6172, 4724, 39648, 22653, 9154, 16490, 1211, 6027, 6156, 5016, 9381, 2423, 39184, 3066, 559, 425, 651, 42963, 14851, 5172, 26895, 2407, 9122, 2407, 4117, 32277, 257, 540, 301, 39896, 6055, 34268, 26890, 3353, 3066, 559, 425, 651, 3353, 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{"start": 566.67, "end": 568.03, "word": " Coagulase", "probability": 0.9466552734375}, {"start": 568.03, "end": 568.97, "word": " بكشف", "probability": 0.888671875}, {"start": 568.97, "end": 569.39, "word": " عنه", "probability": 0.98779296875}, {"start": 569.39, "end": 570.67, "word": " بـ", "probability": 0.631103515625}, {"start": 570.67, "end": 571.21, "word": " Lithium", "probability": 0.35693359375}, {"start": 571.21, "end": 571.85, "word": " Method", "probability": 0.50146484375}, {"start": 571.85, "end": 573.07, "word": " Lithium", "probability": 0.748779296875}, {"start": 573.07, "end": 573.59, "word": " Method", "probability": 0.74609375}, {"start": 573.59, "end": 573.99, "word": " بكشف", "probability": 0.9468994140625}, {"start": 573.99, "end": 574.29, "word": " عنه", "probability": 0.992431640625}, {"start": 574.29, "end": 574.53, "word": " الـ", "probability": 0.817626953125}, {"start": 574.53, "end": 574.83, "word": " Free", "probability": 0.92626953125}, {"start": 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direct لأنه بتحول الفيبرينوجين لفيبرين بواسطة إنزيم الـ Bound Coagulase لكن الـ Free Coagulase indirect لأنه بيتفاعل مع", "tokens": [6027, 39184, 363, 554, 3066, 559, 425, 651, 11933, 1829, 3224, 46740, 24525, 2304, 6055, 7649, 1829, 12174, 1211, 8315, 5296, 39184, 2033, 35313, 479, 15104, 2423, 39184, 11551, 3066, 559, 425, 651, 9881, 12080, 898, 1040, 3066, 559, 425, 651, 5296, 33456, 12174, 1211, 8315, 4724, 1829, 9566, 1211, 3615, 4724, 23557, 33962, 1211, 10632, 19446, 4724, 26895, 2655, 3794, 4587, 15844, 2423, 35632, 2815, 2929, 2423, 5480, 4724, 1829, 30544, 2047, 5296, 33456, 3224, 39894, 5016, 12610, 27188, 1829, 26890, 9957, 29245, 9957, 5296, 5172, 1829, 26890, 9957, 4724, 14407, 3794, 9566, 3660, 36145, 11622, 32640, 2423, 39184, 363, 554, 3066, 559, 425, 651, 44381, 2423, 39184, 11551, 3066, 559, 425, 651, 19523, 5296, 33456, 3224, 4724, 36081, 5172, 995, 30241, 20449], "avg_logprob": -0.1993750057220459, "compression_ratio": 1.7298387096774193, "no_speech_prob": 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"probability": 0.65771484375}, {"start": 650.29, "end": 650.85, "word": " direct", "probability": 0.78759765625}, {"start": 650.85, "end": 651.51, "word": " خطوة", "probability": 0.8642578125}, {"start": 651.51, "end": 651.89, "word": " واحدة", "probability": 0.9720052083333334}, {"start": 651.89, "end": 652.27, "word": " بالتالي", "probability": 0.861865234375}, {"start": 652.27, "end": 652.79, "word": " هياخد", "probability": 0.9505208333333334}, {"start": 652.79, "end": 653.13, "word": " وقت", "probability": 0.983642578125}, {"start": 653.13, "end": 653.57, "word": " أقل", "probability": 0.9169921875}, {"start": 653.57, "end": 654.05, "word": " few", "probability": 0.4423828125}, {"start": 654.05, "end": 654.61, "word": " times", "probability": 0.93896484375}, {"start": 654.61, "end": 655.49, "word": " لكن", "probability": 0.84619140625}, {"start": 655.49, "end": 655.89, "word": " لـ", "probability": 0.903076171875}, {"start": 655.89, "end": 656.05, "word": " free", "probability": 0.85791015625}, {"start": 656.05, "end": 656.79, "word": " coagulase", "probability": 0.9697265625}, {"start": 656.79, "end": 657.19, "word": " خطوة", "probability": 0.9169921875}, {"start": 657.19, "end": 657.35, "word": " تانية", "probability": 0.7762044270833334}, {"start": 657.35, "end": 657.69, "word": " بالتالي", "probability": 0.87568359375}, {"start": 657.69, "end": 658.13, "word": " هياخد", "probability": 0.9788411458333334}, {"start": 658.13, "end": 658.39, "word": " وقت", "probability": 0.991455078125}, {"start": 658.39, "end": 658.81, "word": " أطول", "probability": 0.9812825520833334}, {"start": 658.81, "end": 659.35, "word": " tag", "probability": 0.1395263671875}, {"start": 659.35, "end": 659.75, "word": " time", "probability": 0.78955078125}], "temperature": 1.0}, {"id": 26, "seek": 68943, "start": 660.23, "end": 689.43, "text": "work area بقولي المساحة limited لأنه احنا قلنا هو bound coagulase فقط هيكون على سطح على ال cell wall موجود فبالتالي المساحة اللي ممكن يشتغل عليها limited لكن ال free coagulase broad بتكون واسعة لأنه بيطلع برا الخلية فممكن يشتغل في أي مكان heat tolerance ال bound coagulase heat stable بتحمل درجة الحرارة لكن", "tokens": [1902, 1859, 4724, 39648, 1829, 9673, 3794, 39319, 3660, 5567, 5296, 33456, 3224, 1975, 5016, 8315, 12174, 1211, 8315, 31439, 5472, 598, 559, 425, 651, 6156, 47432, 39896, 30544, 15844, 8608, 9566, 5016, 15844, 2423, 2815, 2929, 3714, 29245, 23328, 6156, 3555, 6027, 2655, 6027, 1829, 9673, 3794, 39319, 3660, 13672, 1829, 3714, 43020, 7251, 8592, 2655, 17082, 1211, 25894, 11296, 5567, 44381, 2423, 1737, 598, 559, 425, 651, 4152, 39894, 30544, 4032, 32277, 27884, 5296, 33456, 3224, 4724, 1829, 9566, 1211, 3615, 4724, 23557, 33962, 1211, 10632, 6156, 2304, 43020, 7251, 8592, 2655, 17082, 1211, 8978, 36632, 3714, 41361, 3738, 23368, 2423, 5472, 598, 559, 425, 651, 3738, 8351, 39894, 35571, 1211, 11778, 47341, 3660, 21542, 2288, 9640, 3660, 44381], "avg_logprob": -0.1493340217676319, "compression_ratio": 1.75, 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"probability": 0.966796875}, {"start": 672.67, "end": 673.05, "word": " يشتغل", "probability": 0.98828125}, {"start": 673.05, "end": 673.39, "word": " عليها", "probability": 0.991455078125}, {"start": 673.39, "end": 673.73, "word": " limited", "probability": 0.6943359375}, {"start": 673.73, "end": 674.21, "word": " لكن", "probability": 0.85400390625}, {"start": 674.21, "end": 674.43, "word": " ال", "probability": 0.82958984375}, {"start": 674.43, "end": 674.63, "word": " free", "probability": 0.873046875}, {"start": 674.63, "end": 675.37, "word": " coagulase", "probability": 0.9541015625}, {"start": 675.37, "end": 675.81, "word": " broad", "probability": 0.400146484375}, {"start": 675.81, "end": 676.95, "word": " بتكون", "probability": 0.93359375}, {"start": 676.95, "end": 677.55, "word": " واسعة", "probability": 0.8776041666666666}, {"start": 677.55, "end": 677.95, "word": " لأنه", "probability": 0.9329427083333334}, {"start": 677.95, "end": 678.51, "word": " بيطلع", "probability": 0.9837890625}, {"start": 678.51, "end": 678.93, "word": " برا", "probability": 0.92041015625}, {"start": 678.93, "end": 679.69, "word": " الخلية", "probability": 0.9715169270833334}, {"start": 679.69, "end": 680.67, "word": " فممكن", "probability": 0.9807942708333334}, {"start": 680.67, "end": 681.23, "word": " يشتغل", "probability": 0.99638671875}, {"start": 681.23, "end": 681.39, "word": " في", "probability": 0.9697265625}, {"start": 681.39, "end": 681.69, "word": " أي", "probability": 0.716796875}, {"start": 681.69, "end": 682.17, "word": " مكان", "probability": 0.991455078125}, {"start": 682.17, "end": 683.37, "word": " heat", "probability": 0.91845703125}, {"start": 683.37, "end": 684.17, "word": " tolerance", "probability": 0.90576171875}, {"start": 684.17, "end": 684.75, "word": " ال", "probability": 0.7255859375}, {"start": 684.75, "end": 685.07, "word": " bound", "probability": 0.69189453125}, {"start": 685.07, "end": 686.09, "word": " coagulase", "probability": 0.962158203125}, {"start": 686.09, "end": 686.73, "word": " heat", "probability": 0.91357421875}, {"start": 686.73, "end": 687.39, "word": " stable", "probability": 0.89794921875}, {"start": 687.39, "end": 688.03, "word": " بتحمل", "probability": 0.9513346354166666}, {"start": 688.03, "end": 688.45, "word": " درجة", "probability": 0.9933268229166666}, {"start": 688.45, "end": 689.11, "word": " الحرارة", "probability": 0.9923095703125}, {"start": 689.11, "end": 689.43, "word": " لكن", "probability": 0.94091796875}], "temperature": 1.0}, {"id": 27, "seek": 71705, "start": 689.81, "end": 717.05, "text": "الـ free coagulase هي الابايل بتأثر بدرجة الحرارة ال bound coagulase بكشف عنه بال slide and tube method لكن ال free coagulase ال tube method لأن ال free ال coagulase بياخد وقت طويل فبالتالي أنا ال tube method هعملها incubation أربع ساعات لكن ال slide بشكل مباشر فبكشف عن ال bound بال slide and tube method", "tokens": [6027, 39184, 1737, 598, 559, 425, 651, 39896, 2423, 16758, 995, 26895, 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{"start": 691.17, "end": 691.45, "word": " هي", "probability": 0.8359375}, {"start": 691.45, "end": 692.19, "word": " الابايل", "probability": 0.588623046875}, {"start": 692.19, "end": 692.87, "word": " بتأثر", "probability": 0.8634440104166666}, {"start": 692.87, "end": 693.83, "word": " بدرجة", "probability": 0.92138671875}, {"start": 693.83, "end": 694.65, "word": " الحرارة", "probability": 0.994384765625}, {"start": 694.65, "end": 695.13, "word": " ال", "probability": 0.5859375}, {"start": 695.13, "end": 695.49, "word": " bound", "probability": 0.392578125}, {"start": 695.49, "end": 696.45, "word": " coagulase", "probability": 0.932373046875}, {"start": 696.45, "end": 697.15, "word": " بكشف", "probability": 0.8580322265625}, {"start": 697.15, "end": 697.53, "word": " عنه", "probability": 0.974853515625}, {"start": 697.53, "end": 697.71, "word": " بال", "probability": 0.8193359375}, {"start": 697.71, "end": 698.15, "word": " slide", "probability": 0.92529296875}, {"start": 698.15, "end": 698.65, "word": " and", "probability": 0.90771484375}, {"start": 698.65, "end": 699.17, "word": " tube", "probability": 0.53515625}, {"start": 699.17, "end": 699.59, "word": " method", "probability": 0.9833984375}, {"start": 699.59, "end": 699.95, "word": " لكن", "probability": 0.78662109375}, {"start": 699.95, "end": 700.17, "word": " ال", "probability": 0.609375}, {"start": 700.17, "end": 700.39, "word": " free", "probability": 0.89453125}, {"start": 700.39, "end": 701.19, "word": " coagulase", "probability": 0.9761962890625}, {"start": 701.19, "end": 701.39, "word": " ال", "probability": 0.3974609375}, {"start": 701.39, "end": 701.73, "word": " tube", "probability": 0.8818359375}, {"start": 701.73, "end": 702.15, "word": " method", "probability": 0.98291015625}, {"start": 702.15, "end": 703.03, "word": " لأن", "probability": 0.91064453125}, {"start": 703.03, "end": 703.39, "word": " ال", "probability": 0.78125}, {"start": 703.39, "end": 703.63, "word": " free", "probability": 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{"start": 710.25, "end": 710.61, "word": " أربع", "probability": 0.8702799479166666}, {"start": 710.61, "end": 711.09, "word": " ساعات", "probability": 0.9713541666666666}, {"start": 711.09, "end": 711.93, "word": " لكن", "probability": 0.951171875}, {"start": 711.93, "end": 712.11, "word": " ال", "probability": 0.73681640625}, {"start": 712.11, "end": 712.41, "word": " slide", "probability": 0.98779296875}, {"start": 712.41, "end": 712.79, "word": " بشكل", "probability": 0.9542643229166666}, {"start": 712.79, "end": 713.25, "word": " مباشر", "probability": 0.99267578125}, {"start": 713.25, "end": 713.87, "word": " فبكشف", "probability": 0.99072265625}, {"start": 713.87, "end": 714.11, "word": " عن", "probability": 0.99169921875}, {"start": 714.11, "end": 714.33, "word": " ال", "probability": 0.927734375}, {"start": 714.33, "end": 714.65, "word": " bound", "probability": 0.89599609375}, {"start": 714.65, "end": 715.09, "word": " بال", "probability": 0.63134765625}, {"start": 715.09, 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"end": 1118.42, "word": " هنا", "probability": 0.31494140625}, {"start": 1118.42, "end": 1119.18, "word": " بكتيريا", "probability": 0.97333984375}, {"start": 1119.18, "end": 1123.22, "word": " يعني", "probability": 0.86669921875}, {"start": 1123.22, "end": 1123.34, "word": " في", "probability": 0.76708984375}, {"start": 1123.34, "end": 1124.08, "word": " كل", "probability": 0.8857421875}, {"start": 1124.08, "end": 1124.42, "word": " drop", "probability": 0.98095703125}, {"start": 1124.42, "end": 1124.62, "word": " هنا", "probability": 0.7802734375}, {"start": 1124.62, "end": 1125.18, "word": " بكتيريا", "probability": 0.9818359375}, {"start": 1125.18, "end": 1125.72, "word": " هحط", "probability": 0.82080078125}, {"start": 1125.72, "end": 1130.86, "word": " وهنا", "probability": 0.839599609375}, {"start": 1130.86, "end": 1131.14, "word": " نفس", "probability": 0.99462890625}, {"start": 1131.14, "end": 1131.76, "word": " البكتيريا", "probability": 0.97607421875}, {"start": 1131.76, 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plasma indicate a positive test بقوللي", "tokens": [2994, 596, 35313, 294, 264, 598, 559, 425, 651, 22564, 293, 3655, 45519, 15771, 294, 264, 1969, 412, 945, 281, 2217, 3949, 4724, 39648, 20292, 4724, 15040, 5016, 19913, 2423, 596, 35313, 13672, 1829, 3714, 29245, 23328, 8978, 3224, 2423, 598, 559, 425, 651, 22564, 2423, 3270, 13672, 1829, 11331, 9566, 36081, 11296, 9154, 29739, 1211, 31377, 15042, 4032, 2423, 3270, 13672, 1829, 11331, 9566, 36081, 3224, 8978, 2423, 1969, 34105, 8978, 18871, 16254, 3714, 15040, 5016, 19913, 9307, 596, 1420, 300, 486, 406, 2890, 48806, 666, 598, 559, 425, 651, 22564, 13330, 257, 3353, 1500, 4724, 39648, 20292], "avg_logprob": -0.16703124314546586, "compression_ratio": 1.7531380753138075, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 1532.37, "end": 1532.71, "word": "for", "probability": 0.3837890625}, {"start": 1532.71, "end": 1533.27, "word": " clumping", "probability": 0.9453125}, {"start": 1533.27, "end": 1533.63, "word": " in", 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"word": " في", "probability": 0.9345703125}, {"start": 1548.23, "end": 1548.35, "word": " ال", "probability": 0.958984375}, {"start": 1548.35, "end": 1549.03, "word": " control", "probability": 0.8564453125}, {"start": 1549.03, "end": 1549.77, "word": " هنا", "probability": 0.3330078125}, {"start": 1549.77, "end": 1549.93, "word": " في", "probability": 0.85009765625}, {"start": 1549.93, "end": 1550.23, "word": " عندي", "probability": 0.796875}, {"start": 1550.23, "end": 1551.01, "word": " ملاحظات", "probability": 0.9876953125}, {"start": 1551.01, "end": 1551.51, "word": " clump", "probability": 0.6304931640625}, {"start": 1551.51, "end": 1551.81, "word": " that", "probability": 0.83251953125}, {"start": 1551.81, "end": 1552.05, "word": " will", "probability": 0.90576171875}, {"start": 1552.05, "end": 1552.37, "word": " not", "probability": 0.9619140625}, {"start": 1552.37, "end": 1552.67, "word": " mix", "probability": 0.96337890625}, {"start": 1552.67, "end": 1553.33, "word": " uniformly", "probability": 0.9609375}, {"start": 1553.33, "end": 1553.75, "word": " into", "probability": 0.736328125}, {"start": 1553.75, "end": 1554.43, "word": " coagulase", "probability": 0.9742431640625}, {"start": 1554.43, "end": 1554.95, "word": " plasma", "probability": 0.96826171875}, {"start": 1554.95, "end": 1555.47, "word": " indicate", "probability": 0.875}, {"start": 1555.47, "end": 1555.97, "word": " a", "probability": 0.97998046875}, {"start": 1555.97, "end": 1556.37, "word": " positive", "probability": 0.92919921875}, {"start": 1556.37, "end": 1557.03, "word": " test", "probability": 0.91455078125}, {"start": 1557.03, "end": 1557.51, "word": " بقوللي", "probability": 0.89404296875}], "temperature": 1.0}, {"id": 58, "seek": 158773, "start": 1557.97, "end": 1587.73, "text": "الـ Positive result بيقول في عيني بيكون Clumping تجمع من اللي فيه بيكون عيني حبيبات Clots أجلوتناشن زي فصائل الدم لكن بالنسبة للـ Negative result بيكون في عيني Uniform suspension أو Smooth suspension بيكون ال suspension كتير ناعم مافي أي Clot تمام هذه النتيجة بتكون Negative result يبقى الـ Positive result بيكون عيني Clumping تجمع لكن الـ Negative Uniform suspension Clumping in both tests", "tokens": [6027, 39184, 46326, 1874, 4724, 1829, 39648, 8978, 6225, 9957, 1829, 4724, 1829, 30544, 2033, 35313, 6055, 7435, 2304, 3615, 9154, 13672, 1829, 8978, 3224, 4724, 1829, 30544, 6225, 9957, 1829, 11331, 21292, 3555, 9307, 2033, 1971, 5551, 7435, 1211, 35473, 1863, 33599, 1863, 30767, 1829, 6156, 9381, 16373, 1211, 32748, 2304, 44381, 20666, 1863, 35457, 3660, 24976, 39184, 43230, 1874, 4724, 1829, 30544, 8978, 6225, 9957, 1829, 1156, 8629, 15771, 34051, 42404, 15771, 4724, 1829, 30544, 2423, 15771, 9122, 2655, 13546, 8717, 995, 25957, 19446, 41185, 36632, 2033, 310, 46811, 10943, 29538, 28239, 31371, 7435, 3660, 39894, 30544, 43230, 1874, 7251, 3555, 4587, 7578, 2423, 39184, 46326, 1874, 4724, 1829, 30544, 6225, 9957, 1829, 2033, 35313, 6055, 7435, 2304, 3615, 44381, 2423, 39184, 43230, 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1573.71, "word": " بيكون", "probability": 0.9464518229166666}, {"start": 1573.71, "end": 1573.85, "word": " ال", "probability": 0.75341796875}, {"start": 1573.85, "end": 1574.21, "word": " suspension", "probability": 0.833984375}, {"start": 1574.21, "end": 1574.69, "word": " كتير", "probability": 0.9464518229166666}, {"start": 1574.69, "end": 1575.35, "word": " ناعم", "probability": 0.8758138020833334}, {"start": 1575.35, "end": 1575.91, "word": " مافي", "probability": 0.88525390625}, {"start": 1575.91, "end": 1576.23, "word": " أي", "probability": 0.751953125}, {"start": 1576.23, "end": 1576.81, "word": " Clot", "probability": 0.706298828125}, {"start": 1576.81, "end": 1577.39, "word": " تمام", "probability": 0.96875}, {"start": 1577.39, "end": 1577.65, "word": " هذه", "probability": 0.63427734375}, {"start": 1577.65, "end": 1578.09, "word": " النتيجة", "probability": 0.91943359375}, {"start": 1578.09, "end": 1578.61, "word": " بتكون", "probability": 0.951904296875}, {"start": 1578.61, "end": 1579.11, "word": " Negative", "probability": 0.818359375}, {"start": 1579.11, "end": 1579.83, "word": " result", "probability": 0.95068359375}, {"start": 1579.83, "end": 1580.25, "word": " يبقى", "probability": 0.959716796875}, {"start": 1580.25, "end": 1580.47, "word": " الـ", "probability": 0.822265625}, {"start": 1580.47, "end": 1580.67, "word": " Positive", "probability": 0.92041015625}, {"start": 1580.67, "end": 1581.07, "word": " result", "probability": 0.9765625}, {"start": 1581.07, "end": 1581.35, "word": " بيكون", "probability": 0.9554036458333334}, {"start": 1581.35, "end": 1581.59, "word": " عيني", "probability": 0.9563802083333334}, {"start": 1581.59, "end": 1582.25, "word": " Clumping", "probability": 0.948486328125}, {"start": 1582.25, "end": 1582.83, "word": " تجمع", "probability": 0.983154296875}, {"start": 1582.83, "end": 1583.09, "word": " لكن", "probability": 0.95556640625}, {"start": 1583.09, "end": 1583.37, "word": " الـ", "probability": 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tube method لأنه هي ال confirmatory", "tokens": [471, 8700, 300, 264, 24128, 8399, 559, 7191, 325, 2486, 412, 307, 412, 307, 2693, 1983, 712, 337, 264, 4137, 598, 559, 425, 525, 1500, 12174, 1211, 8315, 45164, 41850, 6225, 42213, 2655, 27188, 5016, 9381, 4032, 23032, 1211, 34268, 8978, 2423, 6889, 4032, 8978, 2423, 1969, 25961, 18871, 16254, 3353, 3353, 23758, 47525, 1211, 18871, 16472, 3224, 18871, 16254, 8978, 8399, 559, 7191, 325, 2486, 2423, 8399, 559, 7191, 325, 2486, 37893, 3714, 8315, 35457, 24976, 4137, 598, 559, 425, 525, 1500, 5296, 31377, 2304, 5551, 10721, 4117, 3215, 27188, 5016, 9381, 20666, 9917, 3170, 5296, 33456, 3224, 39896, 2423, 9064, 4745], "avg_logprob": -0.2104867837176873, "compression_ratio": 1.7593360995850622, "no_speech_prob": 2.5033950805664062e-06, "words": [{"start": 1587.85, "end": 1588.57, "word": "indicate", "probability": 0.5009918212890625}, {"start": 1588.57, "end": 1588.83, "word": " that", "probability": 0.8974609375}, {"start": 1588.83, "end": 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"word": " في", "probability": 0.85205078125}, {"start": 1604.23, "end": 1606.19, "word": " autoagglutination", "probability": 0.930859375}, {"start": 1606.19, "end": 1606.87, "word": " ال", "probability": 0.8916015625}, {"start": 1606.87, "end": 1607.73, "word": " autoagglutination", "probability": 0.94189453125}, {"start": 1607.73, "end": 1608.03, "word": " مش", "probability": 0.9853515625}, {"start": 1608.03, "end": 1608.55, "word": " مناسب", "probability": 0.9075520833333334}, {"start": 1608.55, "end": 1608.77, "word": " لل", "probability": 0.29638671875}, {"start": 1608.77, "end": 1609.19, "word": " slide", "probability": 0.78515625}, {"start": 1609.19, "end": 1610.01, "word": " coagulast", "probability": 0.9669189453125}, {"start": 1610.01, "end": 1610.25, "word": " test", "probability": 0.9365234375}, {"start": 1610.25, "end": 1610.65, "word": " لازم", "probability": 0.9879557291666666}, {"start": 1610.65, "end": 1611.27, "word": " أأكد", "probability": 0.73382568359375}, {"start": 1611.27, "end": 1611.85, "word": " الفحص", "probability": 0.9889322916666666}, {"start": 1611.85, "end": 1612.07, "word": " بال", "probability": 0.8681640625}, {"start": 1612.07, "end": 1612.55, "word": " tube", "probability": 0.61572265625}, {"start": 1612.55, "end": 1613.03, "word": " method", "probability": 0.97705078125}, {"start": 1613.03, "end": 1613.37, "word": " لأنه", "probability": 0.7620442708333334}, {"start": 1613.37, "end": 1613.59, "word": " هي", "probability": 0.8798828125}, {"start": 1613.59, "end": 1613.85, "word": " ال", "probability": 0.85888671875}, {"start": 1613.85, "end": 1614.59, "word": " confirmatory", "probability": 0.8974609375}], "temperature": 1.0}, {"id": 60, "seek": 162718, "start": 1615.54, "end": 1627.18, "text": "when the autoagglutination is observed واللي بقولنا الـ tube method قلنا لازم أني أنا أشتغلها بدل من ال slide method لأن قلنا ال tube method هو ال confirmatory", "tokens": [18822, 264, 8399, 559, 7191, 325, 2486, 307, 13095, 4032, 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{"start": 1618.66, "end": 1618.86, "word": " الـ", "probability": 0.529296875}, {"start": 1618.86, "end": 1619.04, "word": " tube", "probability": 0.6806640625}, {"start": 1619.04, "end": 1619.32, "word": " method", "probability": 0.9833984375}, {"start": 1619.32, "end": 1619.68, "word": " قلنا", "probability": 0.7890625}, {"start": 1619.68, "end": 1619.98, "word": " لازم", "probability": 0.9453125}, {"start": 1619.98, "end": 1620.18, "word": " أني", "probability": 0.673583984375}, {"start": 1620.18, "end": 1620.28, "word": " أنا", "probability": 0.658203125}, {"start": 1620.28, "end": 1620.88, "word": " أشتغلها", "probability": 0.9786783854166666}, {"start": 1620.88, "end": 1621.22, "word": " بدل", "probability": 0.978759765625}, {"start": 1621.22, "end": 1621.52, "word": " من", "probability": 0.990234375}, {"start": 1621.52, "end": 1621.74, "word": " ال", "probability": 0.6240234375}, {"start": 1621.74, "end": 1622.36, "word": " slide", "probability": 0.92431640625}, {"start": 1622.36, "end": 1622.86, "word": " method", "probability": 0.98779296875}, {"start": 1622.86, "end": 1623.58, "word": " لأن", "probability": 0.843505859375}, {"start": 1623.58, "end": 1623.96, "word": " قلنا", "probability": 0.84130859375}, {"start": 1623.96, "end": 1624.16, "word": " ال", "probability": 0.876953125}, {"start": 1624.16, "end": 1624.52, "word": " tube", "probability": 0.8359375}, {"start": 1624.52, "end": 1625.08, "word": " method", "probability": 0.97900390625}, {"start": 1625.08, "end": 1626.06, "word": " هو", "probability": 0.9765625}, {"start": 1626.06, "end": 1626.34, "word": " ال", "probability": 0.875}, {"start": 1626.34, "end": 1627.18, "word": " confirmatory", "probability": 0.822021484375}], "temperature": 1.0}, {"id": 61, "seek": 165571, "start": 1636.79, "end": 1655.71, "text": "procedure of a tube method خلصنا من ال slide method وعرفنا كيف بنشتغلها وقولنا هي screening method هلأ هنتكلم على ال confirmatory method اللي هو ال tube method اللي بيكشف عن ال free and bound عشان هي أنا قولت يعتبر confirmatory لأنه بيكشف عن ال free", "tokens": [4318, 1232, 540, 295, 257, 9917, 3170, 16490, 1211, 9381, 8315, 9154, 2423, 4137, 3170, 4032, 3615, 28480, 8315, 9122, 33911, 44945, 8592, 2655, 17082, 1211, 11296, 4032, 39648, 8315, 39896, 17732, 3170, 8032, 1211, 10721, 8032, 29399, 4117, 19528, 15844, 2423, 9064, 4745, 3170, 13672, 1829, 31439, 2423, 9917, 3170, 13672, 1829, 4724, 1829, 4117, 8592, 5172, 18871, 2423, 1737, 293, 5472, 6225, 8592, 7649, 39896, 41850, 12174, 12610, 2655, 7251, 34268, 26890, 9064, 4745, 5296, 33456, 3224, 4724, 1829, 4117, 8592, 5172, 18871, 2423, 1737], "avg_logprob": -0.1748934698037126, "compression_ratio": 1.6941747572815533, "no_speech_prob": 2.855062484741211e-05, "words": [{"start": 1636.79, "end": 1637.49, "word": "procedure", "probability": 0.68255615234375}, {"start": 1637.49, "end": 1637.73, "word": " of", "probability": 0.9462890625}, {"start": 1637.73, "end": 1637.95, "word": " a", "probability": 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"probability": 0.79248046875}, {"start": 1641.93, "end": 1642.47, "word": " screening", "probability": 0.82763671875}, {"start": 1642.47, "end": 1643.25, "word": " method", "probability": 0.986328125}, {"start": 1643.25, "end": 1644.61, "word": " هلأ", "probability": 0.7500813802083334}, {"start": 1644.61, "end": 1645.11, "word": " هنتكلم", "probability": 0.956298828125}, {"start": 1645.11, "end": 1645.41, "word": " على", "probability": 0.67724609375}, {"start": 1645.41, "end": 1645.57, "word": " ال", "probability": 0.8935546875}, {"start": 1645.57, "end": 1646.35, "word": " confirmatory", "probability": 0.904541015625}, {"start": 1646.35, "end": 1646.71, "word": " method", "probability": 0.982421875}, {"start": 1646.71, "end": 1646.89, "word": " اللي", "probability": 0.866455078125}, {"start": 1646.89, "end": 1647.21, "word": " هو", "probability": 0.62109375}, {"start": 1647.21, "end": 1647.45, "word": " ال", "probability": 0.478759765625}, {"start": 1647.45, "end": 1647.83, "word": " tube", "probability": 0.82958984375}, {"start": 1647.83, "end": 1648.43, "word": " method", "probability": 0.97705078125}, {"start": 1648.43, "end": 1648.71, "word": " اللي", "probability": 0.9658203125}, {"start": 1648.71, "end": 1649.23, "word": " بيكشف", "probability": 0.8802734375}, {"start": 1649.23, "end": 1649.55, "word": " عن", "probability": 0.98828125}, {"start": 1649.55, "end": 1650.13, "word": " ال", "probability": 0.83349609375}, {"start": 1650.13, "end": 1650.49, "word": " free", "probability": 0.9248046875}, {"start": 1650.49, "end": 1651.01, "word": " and", "probability": 0.94091796875}, {"start": 1651.01, "end": 1651.45, "word": " bound", "probability": 0.8798828125}, {"start": 1651.45, "end": 1652.07, "word": " عشان", "probability": 0.8352864583333334}, {"start": 1652.07, "end": 1652.17, "word": " هي", "probability": 0.6025390625}, {"start": 1652.17, "end": 1652.43, "word": " أنا", "probability": 0.468994140625}, {"start": 1652.43, "end": 1652.79, "word": " قولت", "probability": 0.8248697916666666}, {"start": 1652.79, "end": 1653.29, "word": " يعتبر", "probability": 0.9562174479166666}, {"start": 1653.29, "end": 1654.23, "word": " confirmatory", "probability": 0.9501953125}, {"start": 1654.23, "end": 1654.59, "word": " لأنه", "probability": 0.86279296875}, {"start": 1654.59, "end": 1655.03, "word": " بيكشف", "probability": 0.95517578125}, {"start": 1655.03, "end": 1655.21, "word": " عن", "probability": 0.99462890625}, {"start": 1655.21, "end": 1655.41, "word": " ال", "probability": 0.83251953125}, {"start": 1655.41, "end": 1655.71, "word": " free", "probability": 0.9521484375}], "temperature": 1.0}, {"id": 62, "seek": 167479, "start": 1655.91, "end": 1674.79, "text": "والـ Bound ما بيكشف عن في حال كان فيني Auto-agglutination برضه الـ tube method أنا بستخدمها بدل من ال slide method فبالتالي هو يعتبر confirmatory لكن ال slide فقط بتكشفلي عن ال bound ال bound coagulase", "tokens": [2407, 6027, 39184, 363, 554, 19446, 4724, 1829, 4117, 8592, 5172, 18871, 8978, 11331, 6027, 25961, 8978, 22653, 13738, 12, 559, 7191, 325, 2486, 4724, 43042, 3224, 2423, 39184, 9917, 3170, 41850, 4724, 14851, 9778, 40448, 11296, 47525, 1211, 9154, 2423, 4137, 3170, 6156, 3555, 6027, 2655, 6027, 1829, 31439, 7251, 34268, 26890, 9064, 4745, 44381, 2423, 4137, 6156, 47432, 39894, 4117, 8592, 5172, 20292, 18871, 2423, 5472, 2423, 5472, 598, 559, 425, 651], "avg_logprob": -0.2040624992052714, "compression_ratio": 1.471794871794872, "no_speech_prob": 9.5367431640625e-07, "words": [{"start": 1655.91, "end": 1656.43, "word": "والـ", "probability": 0.88671875}, {"start": 1656.43, "end": 1656.77, "word": " Bound", "probability": 0.779541015625}, {"start": 1656.77, "end": 1657.53, "word": " ما", "probability": 0.76904296875}, {"start": 1657.53, "end": 1658.05, "word": " بيكشف", "probability": 0.8740234375}, {"start": 1658.05, "end": 1658.33, "word": " عن", "probability": 0.89453125}, {"start": 1658.33, "end": 1659.21, "word": " في", "probability": 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0.691162109375}, {"start": 2405.09, "end": 2405.85, "word": " Coagulase", "probability": 0.955078125}, {"start": 2405.85, "end": 2406.27, "word": " منلاحظ", "probability": 0.69012451171875}, {"start": 2406.27, "end": 2406.49, "word": " أنه", "probability": 0.570556640625}, {"start": 2406.49, "end": 2406.59, "word": " في", "probability": 0.96044921875}, {"start": 2406.59, "end": 2406.81, "word": " عندى", "probability": 0.865234375}, {"start": 2406.81, "end": 2407.23, "word": " Smooth", "probability": 0.86572265625}, {"start": 2407.23, "end": 2407.85, "word": " Suspension", "probability": 0.9332682291666666}, {"start": 2407.85, "end": 2408.57, "word": " Uniformة", "probability": 0.81689453125}, {"start": 2408.57, "end": 2408.69, "word": " في", "probability": 0.595703125}, {"start": 2408.69, "end": 2408.93, "word": " عندى", "probability": 0.8955078125}, {"start": 2408.93, "end": 2409.09, "word": " أي", "probability": 0.7646484375}, {"start": 2409.09, "end": 2409.71, "word": " حبيبات", "probability": 0.9949951171875}, {"start": 2409.71, "end": 2409.91, "word": " هذا", "probability": 0.6083984375}, {"start": 2409.91, "end": 2410.37, "word": " بالنسبة", "probability": 0.978759765625}, {"start": 2410.37, "end": 2410.63, "word": " للـ", "probability": 0.775146484375}, {"start": 2410.63, "end": 2410.99, "word": " Slide", "probability": 0.7294921875}, {"start": 2410.99, "end": 2411.31, "word": " Method", "probability": 0.6884765625}, {"start": 2411.31, "end": 2412.13, "word": " لكن", "probability": 0.91796875}, {"start": 2412.13, "end": 2412.35, "word": " للـ", "probability": 0.806640625}, {"start": 2412.35, "end": 2412.59, "word": " Tube", "probability": 0.87841796875}, {"start": 2412.59, "end": 2413.01, "word": " Method", "probability": 0.779296875}, {"start": 2413.01, "end": 2413.59, "word": " الـ", "probability": 0.738037109375}, {"start": 2413.59, "end": 2414.07, "word": " Coagulase", "probability": 0.9779052734375}, {"start": 2414.07, "end": 2414.49, "word": " Positive", "probability": 0.92529296875}, {"start": 2414.49, "end": 2415.05, "word": " منلاحظ", "probability": 0.9713134765625}, {"start": 2415.05, "end": 2415.29, "word": " أن", "probability": 0.71533203125}, {"start": 2415.29, "end": 2415.83, "word": " الـ", "probability": 0.55877685546875}, {"start": 2415.83, "end": 2416.35, "word": " Plasma", "probability": 0.76708984375}, {"start": 2416.35, "end": 2417.09, "word": " جمدت", "probability": 0.9488525390625}], "temperature": 1.0}, {"id": 90, "seek": 243256, "start": 2417.48, "end": 2432.56, "text": "لكن الـ Coagulase Negative أنه مافي عندي Clot و البلازمه ضاله سائل حيث أننا خلصنا الـ Coagulase Test و Catalase Test و حيث أننا خلصنا الـ Chapter لليوم والسلام عليكم ورحمة الله وبركاته", "tokens": [1211, 19452, 2423, 39184, 3066, 559, 425, 651, 43230, 14739, 3224, 19446, 41185, 18871, 16254, 2033, 310, 4032, 29739, 1211, 31377, 2304, 3224, 48812, 6027, 3224, 8608, 16373, 1211, 11331, 1829, 12984, 14739, 8315, 16490, 1211, 9381, 8315, 2423, 39184, 3066, 559, 425, 651, 9279, 4032, 24994, 651, 9279, 4032, 11331, 1829, 12984, 14739, 8315, 16490, 1211, 9381, 8315, 2423, 39184, 18874, 5296, 20292, 20498, 16070, 3794, 37440, 25894, 24793, 4032, 2288, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224], "avg_logprob": -0.3290895179465965, "compression_ratio": 1.6171428571428572, "no_speech_prob": 2.980232238769531e-07, "words": [{"start": 2417.48, "end": 2417.94, "word": "لكن", "probability": 0.7132568359375}, {"start": 2417.94, "end": 2418.14, "word": " الـ", "probability": 0.690185546875}, {"start": 2418.14, "end": 2418.62, "word": " Coagulase", "probability": 0.81280517578125}, {"start": 2418.62, "end": 2418.98, "word": " Negative", "probability": 0.51025390625}, {"start": 2418.98, "end": 2419.36, "word": " أنه", "probability": 0.4407958984375}, {"start": 2419.36, "end": 2419.58, "word": " مافي", "probability": 0.895751953125}, {"start": 2419.58, "end": 2419.82, "word": " عندي", "probability": 0.6514892578125}, {"start": 2419.82, "end": 2420.14, "word": " Clot", "probability": 0.431884765625}, {"start": 2420.14, "end": 2420.4, "word": " و", "probability": 0.68310546875}, {"start": 2420.4, "end": 2421.24, "word": " البلازمه", "probability": 0.633203125}, {"start": 2421.24, "end": 2421.7, "word": " ضاله", "probability": 0.6102294921875}, {"start": 2421.7, "end": 2422.2, "word": " سائل", "probability": 0.9256184895833334}, {"start": 2422.2, "end": 2422.44, "word": " حيث", "probability": 0.6404622395833334}, {"start": 2422.44, "end": 2422.76, "word": " أننا", "probability": 0.4991455078125}, {"start": 2422.76, "end": 2423.38, "word": " خلصنا", "probability": 0.9178466796875}, {"start": 2423.38, "end": 2423.6, "word": " الـ", "probability": 0.601806640625}, {"start": 2423.6, "end": 2424.3, "word": " Coagulase", "probability": 0.934326171875}, {"start": 2424.3, "end": 2424.9, "word": " Test", "probability": 0.7587890625}, {"start": 2424.9, "end": 2425.14, "word": " و", "probability": 0.96630859375}, {"start": 2425.14, "end": 2426.1, "word": " Catalase", "probability": 0.514892578125}, {"start": 2426.1, "end": 2426.48, "word": " Test", "probability": 0.8662109375}, {"start": 2426.48, "end": 2427.06, "word": " و", "probability": 0.5947265625}, {"start": 2427.06, "end": 2427.2, "word": " حيث", "probability": 0.7972819010416666}, {"start": 2427.2, "end": 2427.8, "word": " أننا", "probability": 0.8701171875}, {"start": 2427.8, "end": 2429.16, "word": " خلصنا", "probability": 0.99267578125}, {"start": 2429.16, "end": 2429.38, "word": " الـ", "probability": 0.399658203125}, {"start": 2429.38, "end": 2429.6, "word": " Chapter", "probability": 0.6357421875}, {"start": 2429.6, "end": 2430.16, "word": " لليوم", "probability": 0.9140625}, {"start": 2430.16, "end": 2430.66, "word": " والسلام", "probability": 0.7864583333333334}, {"start": 2430.66, "end": 2431.18, "word": " عليكم", "probability": 0.959228515625}, {"start": 2431.18, "end": 2431.64, "word": " ورحمة", "probability": 0.9166259765625}, {"start": 2431.64, "end": 2431.92, "word": " الله", "probability": 0.97119140625}, {"start": 2431.92, "end": 2432.56, "word": " وبركاته", "probability": 0.97802734375}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 2434.147875, "duration_after_vad": 2142.1024999999927}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/IM6QHSK9qWU_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/IM6QHSK9qWU_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..fcab17de0136d4b8354eafbc7def307ca71800c8
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/IM6QHSK9qWU_raw.srt
@@ -0,0 +1,2008 @@
+1
+00:00:02,250 --> 00:00:05,570
+coagulase test هو الفحص التاني اللي هنتعرف عليه في
+
+2
+00:00:05,570 --> 00:00:09,210
+هذه المحاضرة coagulase test is one of the
+
+3
+00:00:09,210 --> 00:00:12,150
+biochemical test it is very important test in the
+
+4
+00:00:12,150 --> 00:00:15,390
+microbiology هو أحد الفحوصات الكيميائية المهمة في
+
+5
+00:00:15,390 --> 00:00:20,530
+microbiology عشان أشوف هل البكتيريا عندها أنزيم ال
+
+6
+00:00:20,530 --> 00:00:23,370
+coagulase أو لأ أو هده هيساعدني في ال
+
+7
+00:00:23,370 --> 00:00:27,790
+identification the coagulase test identify whether
+
+8
+00:00:27,790 --> 00:00:33,030
+an organism produce the exoenzyme coagulaseالفحص
+
+9
+00:00:33,030 --> 00:00:37,150
+هيعرف لي البكتيريا اللي هتفرز أنزيم الـ Coagulase
+
+10
+00:00:37,150 --> 00:00:42,170
+الـ Coagulase يعتبر Exo-enzyme لكن الكتلاز كان
+
+11
+00:00:42,170 --> 00:00:46,810
+Endo-enzyme which causes the fibrinogen of blood
+
+12
+00:00:46,810 --> 00:00:50,530
+plasma to clot واللي أشهر بيعمل أنزيم الـ
+
+13
+00:00:50,530 --> 00:00:54,830
+Coagulase بيحول الفيبرينوجين الفيبرينوجين موجود في
+
+14
+00:00:54,830 --> 00:00:59,290
+البلازمة هو بروتيين موجود في blood plasma هيحول
+
+15
+00:00:59,290 --> 00:01:05,350
+الفيبرينوجين لفيبرينالـ Fibrin هي الـ Clot تمام،
+
+16
+00:01:05,350 --> 00:01:10,730
+طيب، شو الهدف لما تتكون لـ Clot؟ الجواب موجود في
+
+17
+00:01:10,730 --> 00:01:14,390
+الجملة اللي بعدها organism that produce coagulase
+
+18
+00:01:14,390 --> 00:01:18,510
+can form a protective barrier of fibrin around
+
+19
+00:01:18,510 --> 00:01:22,810
+themselves، بقولّي ال organism اللي بتقدر إنها
+
+20
+00:01:22,810 --> 00:01:26,950
+تنتج أنزيم الـ coagulase هتحول قلنا الـ Fibrinogen
+
+21
+00:01:26,950 --> 00:01:31,420
+لـ Fibrin، لـ Clotهذه الـ clot هتكون زي protective
+
+22
+00:01:31,420 --> 00:01:38,080
+barrier هتكون زي واقي حوالين البكتيريا around
+
+23
+00:01:38,080 --> 00:01:43,020
+themselves البكتيريا بقولي هتتخبى جوا هذه ال clot
+
+24
+00:01:43,020 --> 00:01:48,020
+جوا هذه الفيبرين الجلطة بالتالي making themselves
+
+25
+00:01:48,020 --> 00:01:51,320
+highly resistant to phagocytosis other
+
+26
+00:01:51,320 --> 00:01:57,940
+immunoresponse بقولي هذا ال .. الاشيهيخلّي الـ
+
+27
+00:01:57,940 --> 00:02:01,480
+Immunosystem جهاز المناعة ما يقدر يتعجب عليها
+
+28
+00:02:01,480 --> 00:02:04,540
+بالتالي هتكون highly resistant للفاغو زيتواسيس
+
+29
+00:02:04,540 --> 00:02:09,140
+طبعا الفاغو زيتواسيس من اللي هو ال Immunoresponse
+
+30
+00:02:09,140 --> 00:02:13,200
+لأ ال Immunoresponse بشكل عام كمان and some other
+
+31
+00:02:13,200 --> 00:02:17,940
+antimicrobial agent لو مضاد حيوي بده يقضي عليها مش
+
+32
+00:02:17,940 --> 00:02:22,960
+هيلاقيها هيتكون متخبية البكتيريا جوا ال clotيبقى
+
+33
+00:02:22,960 --> 00:02:26,780
+الـ Clot هتكون زي الـ protective barrier حوالين
+
+34
+00:02:26,780 --> 00:02:30,460
+البكتيريا، هتكون زي حامي حوالين البكتيريا، بالتالي
+
+35
+00:02:30,460 --> 00:02:34,240
+جهاز المناعمة راح يقدر يتعرف عليها ولا المضادات
+
+36
+00:02:34,240 --> 00:02:39,300
+الحاوية significance
+
+37
+00:02:39,300 --> 00:02:43,840
+قلنا إنه بدي أنا أعرف كل فحص شو هو الـ significant
+
+38
+00:02:43,840 --> 00:02:48,380
+لإله، شو أهمية الفحص، بين مين ومين هيفرق هذا الفحص
+
+39
+00:02:49,950 --> 00:02:53,250
+نفرق بين أنه في هذه الـ slide احنا قلنا أهمية
+
+40
+00:02:53,250 --> 00:02:57,110
+الفحص لكن الـ slide اللي قبلها أنه كان protective
+
+41
+00:02:57,110 --> 00:03:01,110
+barrier هذا إيش الهدف من الانزيم أو شو الانزيم
+
+42
+00:03:01,110 --> 00:03:07,210
+هيعمل يبقى الـ هن فائدة الانزيم لكن هن أهمية الفحص
+
+43
+00:03:07,210 --> 00:03:11,550
+الـ coagulase test is used to differentiate the
+
+44
+00:03:11,550 --> 00:03:15,130
+potentially pathogenic species Staphylococcus
+
+45
+00:03:15,130 --> 00:03:19,440
+aureus from other ground positive coccithe usually
+
+46
+00:03:19,440 --> 00:03:24,720
+non pathogenic species احنا قلنا انه لجرام
+
+47
+00:03:24,720 --> 00:03:29,580
+positive ككاى في عندي استفيله ككاى micro ككاى
+
+48
+00:03:29,580 --> 00:03:34,160
+macro ككاى و stripto ككاى فرقنا بينهم بفحص الكتلاز
+
+49
+00:03:34,160 --> 00:03:38,460
+كانت الكتلاز negative stripto لكن الكتلاز positive
+
+50
+00:03:38,460 --> 00:03:44,610
+استفيله ككاى micro ككاى macro ككاى طيبكيف نفرق بين
+
+51
+00:03:44,610 --> 00:03:47,870
+الاستافيلو والمايكرو والمايكرو؟ في فحص سنتعرف عليه
+
+52
+00:03:47,870 --> 00:03:51,710
+في المعمل اللي جاي اللي هو الـ Oxidase الاستافيلو
+
+53
+00:03:51,710 --> 00:03:55,350
+كوكاي هي Oxidase negative لكن المايكرو والمايكرو
+
+54
+00:03:55,350 --> 00:03:58,850
+Oxidase positive فعملت هذا الفحص وطلعت Oxidase
+
+55
+00:03:58,850 --> 00:04:04,490
+negative يبقى البكتيريا الاستافيلو كوكاي طيب
+
+56
+00:04:04,490 --> 00:04:09,660
+الاستافيلو كوكاي تعتبر Genusفي عندي أنا species من
+
+57
+00:04:09,660 --> 00:04:12,700
+الاستفيلو كوكاي في عندي استفيلو كوكاس aureus في
+
+58
+00:04:12,700 --> 00:04:15,360
+عندي استفيلو كوكاس epidermidis في عندي استفيلو
+
+59
+00:04:15,360 --> 00:04:19,240
+كوكاس saprophyticas كيف بدأ أفرق بين الاستفيلو
+
+60
+00:04:19,240 --> 00:04:23,440
+كوكاس species بقولني من خلال فحص الـ Coagulase
+
+61
+00:04:23,440 --> 00:04:28,120
+الاستفيلو كوكاس aureus هي تعتبر positive Coagulase
+
+62
+00:04:28,120 --> 00:04:32,240
+positive لكن الـ non pathogenic species اللي هي
+
+63
+00:04:32,240 --> 00:04:35,220
+الاستفيلو كوكاس epidermidis و الـ saprophyticas هي
+
+64
+00:04:35,220 --> 00:04:39,290
+Coagulase negativeالـ Staphylococcus aureus
+
+65
+00:04:39,290 --> 00:04:42,310
+potentially pathogenic in human and animal هي
+
+66
+00:04:42,310 --> 00:04:44,990
+تعتبر pathogenic لكن الـ Staphylococcus
+
+67
+00:04:44,990 --> 00:04:49,150
+epidermidis والـ Sabrophyticus تعتبر over-chinstic
+
+68
+00:04:49,150 --> 00:04:53,710
+انتهازية يعني بتنتهز ضعف جهاز المنارة بسميها
+
+69
+00:04:53,710 --> 00:04:58,130
+nosocomial بتسبب الـ nosocomial infection أو ال
+
+70
+00:04:58,130 --> 00:05:01,150
+hospital acquired infection أو ال health care
+
+71
+00:05:01,150 --> 00:05:05,730
+associated infection هذه نفس المسميات يعني نفس ال
+
+72
+00:05:05,730 --> 00:05:10,300
+nosocomial infectionيبقى فحص الـ Coagulase أهميته
+
+73
+00:05:10,300 --> 00:05:14,160
+أنه بيفرق للاستفيلوكوكس Aureus اللي هي بتكون
+
+74
+00:05:14,160 --> 00:05:17,140
+Coagulase positive عن باقي ال species من
+
+75
+00:05:17,140 --> 00:05:20,220
+الاستفيلوكوكس اللي هي بتكون negative الاستفيلوكوكس
+
+76
+00:05:20,220 --> 00:05:28,020
+Epidermidis و الاستفيلوكوكس Sabrophytic منلاحظ
+
+77
+00:05:28,020 --> 00:05:32,140
+بالصورة الاستفيلوكوكس قلنا هي استفيلو عنقودية فما
+
+78
+00:05:32,140 --> 00:05:36,470
+منلاحظ أنهاعلى شكل عمقود العينبو أكيد هي الـ Gram
+
+79
+00:05:36,470 --> 00:05:39,830
+-positive ككاى Gram-positive مخد اللون الviolet
+
+80
+00:05:39,830 --> 00:05:46,050
+وككاى شكلها كراو Types of Coagulase بقول لي في
+
+81
+00:05:46,050 --> 00:05:50,650
+أنواع لـ Coagulase Coagulase enzyme بقول لي فيه
+
+82
+00:05:50,650 --> 00:05:55,150
+بيكون بشكلين ممكن يكون Bound Coagulase ممكن يكون
+
+83
+00:05:55,150 --> 00:06:00,660
+Free Coagulaseالـ Bound Coagulase also called the
+
+84
+00:06:00,660 --> 00:06:04,460
+clumping factor يبقى الـ Bound له اسم تاني اللي هو
+
+85
+00:06:04,460 --> 00:06:11,480
+ال Clumping Factor is attached to bacterial cell
+
+86
+00:06:11,480 --> 00:06:15,640
+wall and react directly with the fibrinogen in
+
+87
+00:06:15,640 --> 00:06:19,420
+plasma بقولي الـ Bound من اسمه بتفرزه الخلية
+
+88
+00:06:19,420 --> 00:06:25,200
+البكتيريةوبطلع لبره لكن بيظل مرتبط بال cell wall
+
+89
+00:06:25,200 --> 00:06:28,920
+is attached to the bacterial cell wall بيظل مرتبط
+
+90
+00:06:28,920 --> 00:06:31,940
+بال cell wall قولنا إنه إيه له اسم تاني اللي هو ال
+
+91
+00:06:31,940 --> 00:06:35,760
+clamping factor and react directly with the
+
+92
+00:06:35,760 --> 00:06:39,280
+fibrinogen in plasma بيقول ليه بيصير التفاعل بشكل
+
+93
+00:06:39,280 --> 00:06:43,560
+directly وده اللي هيفرق إيه بينه وبين الفريق
+
+94
+00:06:43,560 --> 00:06:48,070
+التفاعل بيكون direct with the fibrinogenالـ
+
+95
+00:06:48,070 --> 00:06:51,270
+Substrate اللي هيشتغل عليها الـ enzyme سواء Free
+
+96
+00:06:51,270 --> 00:06:56,350
+أو Bound هي الـ Fibrinogen اللي موجودة بتكون في
+
+97
+00:06:56,350 --> 00:07:01,410
+البلازمة نحنا هنستخدم اللي هي هنفعل ال كولونيه
+
+98
+00:07:01,410 --> 00:07:04,790
+البكتيريا مع البلازمة البلازمة الموجودة فيها الـ
+
+99
+00:07:04,790 --> 00:07:08,590
+Fibrinogen بقوللي إن التفاعل بيصير بشكل مباشر
+
+100
+00:07:08,590 --> 00:07:12,210
+هيتفاعل مع الـ Fibrinogen الـ Substrate عندي هي
+
+101
+00:07:12,210 --> 00:07:15,250
+الـ Fibrinogen هيشتغل عليها الـ Coagulase بشكل
+
+102
+00:07:15,250 --> 00:07:20,290
+مباشرهتتحول الـ Fibrinogen بواسطة انزيم الـ
+
+103
+00:07:20,290 --> 00:07:24,310
+Coagulase لـ Fibrin لـ Clot يبقى في عندي انا هياها
+
+104
+00:07:24,310 --> 00:07:28,890
+في one steps خطوة واحدة لـ Fibrinogen بواسطة انزيم
+
+105
+00:07:28,890 --> 00:07:33,330
+الـ Coagulase اتحول لـ Fibrin في خطة واحدة بالتالي
+
+106
+00:07:33,330 --> 00:07:37,330
+الـ Bound Coagulase بيكون بشكل Rapid بشكل سريع
+
+107
+00:07:37,330 --> 00:07:42,350
+فبالتالي أنا بكشف عنه بالـ Slide Method فقط يبقى
+
+108
+00:07:42,350 --> 00:07:45,250
+لو بدنا نقارن الـ Bound Coagulase اسمه Clumping
+
+109
+00:07:45,250 --> 00:07:49,880
+Factorبصير بشكل directly مباشر المعادلة
+
+110
+00:07:49,880 --> 00:07:54,700
+الفيبرونيجين بتحول لفيبرين لكلو 91 steps الإشي
+
+111
+00:07:54,700 --> 00:07:58,960
+بيكون بشكل rapid الوقت بيكون سريع فبالتالي فقط
+
+112
+00:07:58,960 --> 00:08:03,860
+بكشف عنه بالـ slide method لكن الـ free coagulase
+
+113
+00:08:03,860 --> 00:08:08,720
+is an extracellular enzyme بتفرز الخلية وبيطلع
+
+114
+00:08:08,720 --> 00:08:13,100
+خارج الخلية ما بيلزق في ال bacterial cell wall زي
+
+115
+00:08:13,100 --> 00:08:17,200
+الـ bound coagulaseفبالتالي هو يُعتبر
+
+116
+00:08:17,200 --> 00:08:21,380
+Extracellular enzyme released from the cell that
+
+117
+00:08:21,380 --> 00:08:26,200
+react with a plasma component called coagulase
+
+118
+00:08:26,200 --> 00:08:29,400
+reacting factor بقولّي التفاعل بيصير بشكل
+
+119
+00:08:29,400 --> 00:08:33,440
+indirectly بشكل غير مباشر على عكس ال bound كيف
+
+120
+00:08:33,440 --> 00:08:37,900
+بيصير بشكل غير مباشر؟ بقولّي قبل ما يصير التفاعل
+
+121
+00:08:37,900 --> 00:08:42,240
+ال free coagulase بتفاعل مع مكوّن من مكونات
+
+122
+00:08:42,240 --> 00:08:46,850
+البلازمة اسمه coagulase reacting factorهذا اختصاره
+
+123
+00:08:46,850 --> 00:08:52,030
+الـ Coagulase بتفعل مع الـ Coagulase Reacting
+
+124
+00:08:52,030 --> 00:08:57,350
+Factor وهيحول لبروثرومبين لثرومبين هيتحول
+
+125
+00:08:57,350 --> 00:09:03,170
+لبروثرومبين لثرومبين بعدها لثرومبين هو اللي هيحول
+
+126
+00:09:03,170 --> 00:09:07,710
+الفيبرينوجين لفيبرين لفيبرين لكلوت يبقى عندى انا
+
+127
+00:09:07,710 --> 00:09:14,050
+two step ال Coagulaseعندما اتفاعل مع الـ Coagulase
+
+128
+00:09:14,050 --> 00:09:18,410
+Reacting Factor حوّلي البرو ثرمبن لثرمبن بعد كده
+
+129
+00:09:18,410 --> 00:09:21,970
+الثرمبن هو اللي حوّل الفيبرينوجين لفيبريني يبقى في
+
+130
+00:09:21,970 --> 00:09:26,670
+عندي two steps هياخد وقت طويل فبالتالي الـ Free
+
+131
+00:09:26,670 --> 00:09:33,070
+Coagulase بكشف عنه بـ Lithium Method Lithium
+
+132
+00:09:33,070 --> 00:09:38,310
+Method بكشف عنه الـ Free Coagulase هذا بالنسبة لـ
+
+133
+00:09:38,520 --> 00:09:42,240
+الأنواع أو الـ form of coagulase فى عندي bound و
+
+134
+00:09:42,240 --> 00:09:46,740
+فى عندي ال free coagulase بقول ليه ال reaction
+
+135
+00:09:46,740 --> 00:09:53,020
+النتيجة نفس اللى هى ال bound coagulase لكن بتحول ل
+
+136
+00:09:53,020 --> 00:09:56,540
+-ibrothrombin بعدين بتحول ل-fibrinogen و بيصير فى
+
+137
+00:09:56,540 --> 00:09:57,540
+عندي Eclot
+
+138
+00:10:06,720 --> 00:10:10,280
+هنا في مقارنة بين ال bound coagulates و ال free
+
+139
+00:10:10,280 --> 00:10:16,100
+coagulatesالـ Bound Coagulase إيه له اسم تاني قلنا
+
+140
+00:10:16,100 --> 00:10:18,400
+لـ Clumping Factor الـ Free Coagulase
+
+141
+00:10:18,400 --> 00:10:23,460
+Extracellular Coagulase لأن قلنا بيطلع برا الخلية
+
+142
+00:10:23,460 --> 00:10:27,620
+ما بيلتسق على ال bacterial cell wall ال reaction
+
+143
+00:10:27,620 --> 00:10:31,580
+بيكون direct لأنه بتحول الفيبرينوجين لفيبرين
+
+144
+00:10:31,580 --> 00:10:35,160
+بواسطة إنزيم الـ Bound Coagulase لكن الـ Free
+
+145
+00:10:35,160 --> 00:10:40,190
+Coagulase indirect لأنه بيتفاعل معالـ coagulase
+
+146
+00:10:40,190 --> 00:10:43,730
+reacting factor لـ free coagulase بالتالي بيحوله
+
+147
+00:10:43,730 --> 00:10:46,510
+لبروثرومبين لاثرومبين بعدها لاثرومبين هو اللي
+
+148
+00:10:46,510 --> 00:10:51,510
+بيحول الفيبرينوجين لفيبرين بما انه direct خطوة
+
+149
+00:10:51,510 --> 00:10:56,050
+واحدة بالتالي هياخد وقت أقل few times لكن لـ free
+
+150
+00:10:56,050 --> 00:10:59,350
+coagulase خطوة تانية بالتالي هياخد وقت أطول tag
+
+151
+00:10:59,350 --> 00:11:04,210
+timework area بقولي المساحة limited لأنه احنا قلنا
+
+152
+00:11:04,210 --> 00:11:09,030
+هو bound coagulase فقط هيكون على سطح على ال cell
+
+153
+00:11:09,030 --> 00:11:13,390
+wall موجود فبالتالي المساحة اللي ممكن يشتغل عليها
+
+154
+00:11:13,390 --> 00:11:17,550
+limited لكن ال free coagulase broad بتكون واسعة
+
+155
+00:11:17,550 --> 00:11:23,370
+لأنه بيطلع برا الخلية فممكن يشتغل في أي مكان heat
+
+156
+00:11:23,370 --> 00:11:28,030
+tolerance ال bound coagulase heat stable بتحمل
+
+157
+00:11:28,030 --> 00:11:32,190
+درجة الحرارة لكنالـ free coagulase هي الابايل
+
+158
+00:11:32,190 --> 00:11:37,530
+بتأثر بدرجة الحرارة ال bound coagulase بكشف عنه
+
+159
+00:11:37,530 --> 00:11:41,390
+بال slide and tube method لكن ال free coagulase ال
+
+160
+00:11:41,390 --> 00:11:45,270
+tube method لأن ال free ال coagulase بياخد وقت
+
+161
+00:11:45,270 --> 00:11:50,250
+طويل فبالتالي أنا ال tube method هعملها incubation
+
+162
+00:11:50,250 --> 00:11:54,330
+أربع ساعات لكن ال slide بشكل مباشر فبكشف عن ال
+
+163
+00:11:54,330 --> 00:12:00,020
+bound بال slide and tube methodالفحص يعتبر الـ
+
+164
+00:12:00,020 --> 00:12:02,920
+bound coagulase بسمي screening لكن الـ free
+
+165
+00:12:02,920 --> 00:12:09,060
+coagulase confirmatory لأن الـ bound اللي بكشف في
+
+166
+00:12:09,060 --> 00:12:13,420
+ال slide and tube method لكن ال free في ال tube
+
+167
+00:12:13,420 --> 00:12:17,280
+method يبقى ال bound coagulase يعتبر screening لكن
+
+168
+00:12:17,280 --> 00:12:19,840
+ال free coagulase يعتبر confirmatory
+
+169
+00:12:28,350 --> 00:12:31,510
+كيف بدى انا اكشف او كيف بدى اشتغل فحص الـ
+
+170
+00:12:31,510 --> 00:12:34,290
+Coagulase قولنا في درايين دى طريقتين الـ tube
+
+171
+00:12:34,290 --> 00:12:39,390
+method هتكشفلي عن وجود ال presence of bound or
+
+172
+00:12:39,390 --> 00:12:43,250
+free لكن ال slide method فقط هتكشفلي على ال bound
+
+173
+00:12:43,250 --> 00:12:47,050
+coagulase فبالتالي ال tube method تعتبر
+
+174
+00:12:47,050 --> 00:12:50,370
+confirmatory لكن ال slide method تعتبر screening
+
+175
+00:12:50,370 --> 00:12:56,680
+test فقط بتكشفلي على ال bound coagulaseعشان هيك لو
+
+176
+00:12:56,680 --> 00:13:00,600
+عملت slide method وطلعت negative لازم أؤكد بال
+
+177
+00:13:00,600 --> 00:13:04,620
+tube method عشان أكشف عن ال free coagulase اللي ما
+
+178
+00:13:04,620 --> 00:13:13,940
+قدرت slide method تكشف ليه عنه بالنسبة
+
+179
+00:13:13,940 --> 00:13:17,460
+لل slide method هنشرح كيف slide method slide
+
+180
+00:13:17,460 --> 00:13:19,760
+method بقول ب GB slide
+
+181
+00:13:22,080 --> 00:13:46,120
+وبتعملي بتحطي drop من الـ relative plasma مع
+
+182
+00:13:46,120 --> 00:13:48,080
+touch of bacteria
+
+183
+00:14:10,280 --> 00:14:14,120
+يبقى طريقة ال slide method drop of rabbit plasma
+
+184
+00:14:14,120 --> 00:14:17,200
+مع touch of بكتيريا اللى انا بدي اكشف هل عندها ال
+
+185
+00:14:17,200 --> 00:14:21,940
+coagulase او لأ بعمل mix و بشوف النتيجة السؤال هان
+
+186
+00:14:21,940 --> 00:14:26,460
+انا ليش استخدمت plasma ما استخدمت serum السؤال
+
+187
+00:14:26,460 --> 00:14:29,820
+التانى ليش استخدمت rabbit plasma ما استخدمت human
+
+188
+00:14:29,820 --> 00:14:33,850
+plasmaأول شيء لماذا استخدمنا إبلازمة وما استخدمنا
+
+189
+00:14:33,850 --> 00:14:37,110
+سيرام الـ substrate اللي هيشتغل عليها ال enzyme هي
+
+190
+00:14:37,110 --> 00:14:40,690
+الفيبرينوجين الفيبرينوجين موجود في البلازمة مش
+
+191
+00:14:40,690 --> 00:14:44,910
+موجود في السيرام كيف أنا أحصل على إبلازمة بسحب عين
+
+192
+00:14:44,910 --> 00:14:49,470
+الدم و بحطها بتيوب فيه anticoagulant مانع للتجلط
+
+193
+00:14:49,470 --> 00:14:53,550
+عشان مايصير في عندي تجلط كلوت لأنه لو صار في عندي
+
+194
+00:14:53,550 --> 00:14:57,920
+تجلط هيتحول الفيبرينوجين لفيبرين فبالتاليهك
+
+195
+00:14:57,920 --> 00:15:01,900
+الفيبرينوجين تم استهلاكه طب أنا بديه الفيبرينوجين
+
+196
+00:15:01,900 --> 00:15:06,020
+ك substrate فبالتالي أنا بستخدم البلازما لأن
+
+197
+00:15:06,020 --> 00:15:09,880
+البلازما ما بيصير في عندى cloth ما بتتحول
+
+198
+00:15:09,880 --> 00:15:13,340
+الفيبرينوجين لفيبرين الفيبرينوجين بيضل موجود as
+
+199
+00:15:13,340 --> 00:15:17,780
+substrate لكن ال serum ال serum كيف بدي أحصل على
+
+200
+00:15:17,780 --> 00:15:21,200
+serum بس بعين الدم و بحطها في plain ال tube عادي
+
+201
+00:15:21,200 --> 00:15:26,770
+مافيها مانع للتجلط بتركها تتجلط بعدين بفصلهابيتحول
+
+202
+00:15:26,770 --> 00:15:30,330
+الفيبرينوجين لفيبرين يعني تم استهلاك الفيبرينوجين
+
+203
+00:15:30,330 --> 00:15:34,210
+مش موجود عند الفيبرينوجين في السيرام بالتالي انا
+
+204
+00:15:34,210 --> 00:15:37,950
+مابقدر اشتغل عليه لأنه انا بدي الفيبرينوجين as
+
+205
+00:15:37,950 --> 00:15:42,030
+substrate عشان يشتغل عليه ال enzyme عشان هيك لازم
+
+206
+00:15:42,030 --> 00:15:47,660
+يكون plasma مش serumبالنسبة لـ Rabbit Plasma ليش
+
+207
+00:15:47,660 --> 00:15:51,700
+أنا استخدمت Rabbit Plasma لأن لـ Human Plasma في
+
+208
+00:15:51,700 --> 00:15:55,340
+عندي Inhibitor للإنزايم للـ Coagulase Enzyme
+
+209
+00:15:55,340 --> 00:16:00,040
+بالتالي ممكن يعطيني False Negative Result يبقى
+
+210
+00:16:00,040 --> 00:16:02,860
+احنا حطينا Drop of Rabbit Plasma ال Rabbit Plasma
+
+211
+00:16:02,860 --> 00:16:06,500
+موجود في ال substrate اللي هو الفيبرينوجين
+
+212
+00:16:22,650 --> 00:16:27,030
+Touch of bacteria اللى موجود أو مش موجود حسب
+
+213
+00:16:27,030 --> 00:16:29,650
+البكتيريا اللى فيها ال enzyme اللى هو الـ
+
+214
+00:16:29,650 --> 00:16:30,290
+Coagulase
+
+215
+00:16:39,470 --> 00:16:43,890
+بعمل mix عشان يشتغل ال enzyme على ال substrate لو
+
+216
+00:16:43,890 --> 00:16:48,010
+موجود هشوف ياندي eclot هشوف agglutination زي
+
+217
+00:16:48,010 --> 00:16:51,510
+حبيبات زي اللي بنشوفها بفحص فصائل الدم يبقى هاي
+
+218
+00:16:51,510 --> 00:16:55,630
+النتيجة positive هي coagulase positive لكن لو مش
+
+219
+00:16:55,630 --> 00:17:00,670
+موجود ال enzyme مش هشوف أي حبيبات هيكون في عندي
+
+220
+00:17:00,670 --> 00:17:05,530
+smooth suspensionبقول لي place a drop of distilled
+
+221
+00:17:05,530 --> 00:17:09,150
+water or saline near the drop of plasma as control
+
+222
+00:17:09,150 --> 00:17:24,110
+نكتبها negative control بقول
+
+223
+00:17:24,110 --> 00:17:27,950
+لي أنت أول إشي بتحط drop من ال rabbit plasma و
+
+224
+00:17:27,950 --> 00:17:31,550
+بعدين بتحط ال touch of bacteria بقولنا ممكن أن نحط
+
+225
+00:17:31,800 --> 00:17:35,460
+أو لازم نحط pistol water أو normal saline جنب
+
+226
+00:17:35,460 --> 00:17:40,340
+الربط في البلازما هذا هيكون as negative control
+
+227
+00:17:40,340 --> 00:17:56,420
+كيف يعني أنا جبت ال slide قلنا
+
+228
+00:17:56,420 --> 00:17:58,600
+اضيف drop من الربط في البلازما
+
+229
+00:18:05,830 --> 00:18:12,910
+هان وهان كمان ال drop من distilled water
+
+230
+00:18:12,910 --> 00:18:18,210
+او
+
+231
+00:18:18,210 --> 00:18:19,850
+normal saline
+
+232
+00:18:28,620 --> 00:18:30,960
+الـ drop من الـ rabbit plasma والـ drop من الـ
+
+233
+00:18:30,960 --> 00:18:33,220
+distal water أو الـ normal saline بعدين بجيب
+
+234
+00:18:33,220 --> 00:18:35,900
+البكتيريا اللى انا بدي اكشف هل موجودة عندها
+
+235
+00:18:35,900 --> 00:18:43,220
+الانزيم ولا لأ و بحط هنا بكتيريا يعني
+
+236
+00:18:43,220 --> 00:18:50,860
+في كل drop هنا بكتيريا هحط وهنا
+
+237
+00:18:50,860 --> 00:18:53,200
+نفس البكتيريا اللى انا بدي اكشف عليها
+
+238
+00:19:18,370 --> 00:19:22,050
+يبقى بنحط رابط بلازما drop من الـ distal water
+
+239
+00:19:22,050 --> 00:19:25,150
+بجيب البكتيريا اللى بدى أكشف عنها هل عندها الانزيم
+
+240
+00:19:25,150 --> 00:19:28,690
+ولا لأ بحطها drop و بعمل mix مع رابط البلازما و
+
+241
+00:19:28,690 --> 00:19:31,730
+بحطها كولونى من البكتيريا اللى أنا بدى أكشف عندها
+
+242
+00:19:31,730 --> 00:19:36,090
+الانزيم نفس اللى حطيتها هنا و بعمل mix طيب ليش هذه
+
+243
+00:19:36,090 --> 00:19:38,710
+الفكرة قولنا هذا ال distal water أو ال normal
+
+244
+00:19:38,710 --> 00:19:41,470
+saline مع البكتيريا اللى بدى أكشف عنها تعتبر as
+
+245
+00:19:41,470 --> 00:19:46,870
+negative control هلأ لو البكتيريا عندها ال enzyme
+
+246
+00:19:46,870 --> 00:19:51,850
+لو البكتيرياهي عندها ال enzyme هكيد هتشتغل على ال
+
+247
+00:19:51,850 --> 00:19:54,570
+substrate اللي هي الفيبرينوجين اللي موجود في
+
+248
+00:19:54,570 --> 00:20:00,490
+البلازما وهتعطيني هتحول الفيبرينوجين لفيبرين هيصير
+
+249
+00:20:00,490 --> 00:20:03,970
+في عندي agglutination هيكون في عندي clot يبقى
+
+250
+00:20:03,970 --> 00:20:09,770
+بتكون النتيجة لو positive هي هتكون positive لو
+
+251
+00:20:09,770 --> 00:20:14,230
+عندها ال enzyme هكيد هي هتكون positive لكن لو أنا
+
+252
+00:20:14,230 --> 00:20:18,360
+عملتنفس البكتيريا اللى عندها الانزيم عملت mix مع
+
+253
+00:20:18,360 --> 00:20:22,980
+ديستلواتر أكيد هتكون النتيجة هان نيجاتف مش هيكون
+
+254
+00:20:22,980 --> 00:20:28,980
+فيه عندى أي إكلوت أي حبيبات ليش لأنه مافيه عند
+
+255
+00:20:28,980 --> 00:20:31,760
+السبسترات اللى هيشتغل عليها الانزيم هان فيه عند
+
+256
+00:20:31,760 --> 00:20:34,060
+البلازما موجود فيها الفيبرينجين لكن ال normal
+
+257
+00:20:34,060 --> 00:20:37,080
+saline أو الديستلواتر مش موجود في السبسترات اللى
+
+258
+00:20:37,080 --> 00:20:39,720
+هو الفيبرينجين اللى هيشتغل عليها الانزيم يعني حتى
+
+259
+00:20:39,720 --> 00:20:44,030
+لو كان الانزيمموجود في البكتيريا اكيد في ال
+
+260
+00:20:44,030 --> 00:20:47,450
+negative control هتكون negative ماهيكون في عندي
+
+261
+00:20:47,450 --> 00:20:51,130
+agglutination طيب هذه في الحال كانت هي positive
+
+262
+00:20:51,130 --> 00:20:55,750
+result خلينا نكتب هان وكان في عندي هان positive
+
+263
+00:20:55,750 --> 00:20:59,830
+result لو كان عندها ال enzyme
+
+264
+00:21:11,410 --> 00:21:15,190
+طيب لو كانت ما عندها البكتيريا لو جبت بكتيريا
+
+265
+00:21:15,190 --> 00:21:18,990
+تانية هذه البكتيريا هي negative لل coagulase ما
+
+266
+00:21:18,990 --> 00:21:23,470
+عندها الانزيم ال coagulase هعمل mix مع ال rabbit
+
+267
+00:21:23,470 --> 00:21:27,410
+plasma فأكيد هتكون النتيجة هان مش مش موجود الانزيم
+
+268
+00:21:27,410 --> 00:21:31,250
+لكن موجود ال substrate لكن مش هيصير في عندي تفاعل
+
+269
+00:21:31,250 --> 00:21:37,630
+هتكون negative لو حطيتها مع الالنظام الطبيعي
+
+270
+00:21:37,630 --> 00:21:41,770
+لايوجد فيه أنزيمات أو سبسترات فهو لن يكون فيه
+
+271
+00:21:41,770 --> 00:21:47,850
+اغلوتناشن فهو نيجاتيف يبقى هنا في حال كانت نيجاتيف
+
+272
+00:21:47,850 --> 00:21:48,210
+result
+
+273
+00:22:05,780 --> 00:22:09,360
+يبقى في كلتال حالتان الـ negative control لازم
+
+274
+00:22:09,360 --> 00:22:14,180
+يكون عندها نيجاتيف لش لأنه ال normal saline مش
+
+275
+00:22:14,180 --> 00:22:18,040
+موجود في عندى ال substrate اللى هي ال fibrinogen
+
+276
+00:22:18,040 --> 00:22:23,240
+اللى هيشتغل عليها ال enzyme طيب لو كانت النتيجة
+
+277
+00:22:23,240 --> 00:22:28,440
+عملت جبت بكتيريا وحطيتها مع البلازمه وطلعت
+
+278
+00:22:28,440 --> 00:22:32,960
+positive وحطيتها
+
+279
+00:22:32,960 --> 00:22:41,650
+مع ال normal saline وبرضه طلعتpositive احنا
+
+280
+00:22:41,650 --> 00:22:45,070
+قلنا انه ال negative control لازم يكون negative
+
+281
+00:22:45,070 --> 00:22:48,490
+لأنه ال normal cell line ماعندي فيبرينوجين مش
+
+282
+00:22:48,490 --> 00:22:51,350
+موجود ال substrate اللي هيشتغل عليه ال enzyme طيب
+
+283
+00:22:51,350 --> 00:22:54,550
+من وين صار عندي ال agglutination في ال negative
+
+284
+00:22:54,550 --> 00:22:58,350
+control و اللي هنا بيكون في عندي مشكلة قلنا ممنوع
+
+285
+00:22:58,350 --> 00:23:01,350
+تطلع بال negative control positive لأنه مافي
+
+286
+00:23:01,350 --> 00:23:03,990
+substrate الفيبرينوجين اللي هيشتغل عليه ال enzyme
+
+287
+00:23:04,540 --> 00:23:08,900
+طب ايش السبب اللي خلّى صار عندى فيه agglutination
+
+288
+00:23:08,900 --> 00:23:13,320
+بقول السبب ان البكتيريا بتعمل autoagglutination
+
+289
+00:23:13,320 --> 00:23:19,460
+يعني بشكل ذات بدون وجود enzyme autoagglutination
+
+290
+00:23:33,940 --> 00:23:39,480
+في عندي تفسيرها ان في عندي auto agglutination مش
+
+291
+00:23:39,480 --> 00:23:43,860
+ناتج عن وجود إنزايم الكواليولازي هي بتفرز clumping
+
+292
+00:23:43,860 --> 00:23:50,000
+factor فبيصير في عندي agglutination يبقى بالثانبال
+
+293
+00:23:50,000 --> 00:23:53,020
+انا مش عارفة في هذه العينة لو كانت في ال .. لو
+
+294
+00:23:53,020 --> 00:23:55,600
+كانت انا خلطت بـ Irreversible plasma مع البكتيريا
+
+295
+00:23:55,600 --> 00:23:59,290
+في هذه الحالةأنا مش عارفة هل البكتيريا عندها انزيم
+
+296
+00:23:59,290 --> 00:24:03,190
+الـ coagulase وعملة agglutination ولا ما عندها
+
+297
+00:24:03,190 --> 00:24:07,970
+وكان ال agglutination اللي ظهر في ال sample من ال
+
+298
+00:24:07,970 --> 00:24:13,140
+autoagglutination بقوللي الحل في هايإن أنا عشان
+
+299
+00:24:13,140 --> 00:24:15,780
+أعرف في هذه الحلقة لو طلع positive في positive
+
+300
+00:24:15,780 --> 00:24:20,060
+لازم أتأكد بالـ tube method قولنا الـ tube method
+
+301
+00:24:20,060 --> 00:24:24,440
+هي confirmatory تأكيدية لـ slides screening فلازم
+
+302
+00:24:24,440 --> 00:24:27,160
+أنا أعمل ال tube method اللي هنتعرف عليها كمان
+
+303
+00:24:27,160 --> 00:24:33,140
+شوية ال tube method هي اللي هتعرفني هل هو auto
+
+304
+00:24:33,140 --> 00:24:36,800
+agglutination ولا لأ لو طلعت positive يبقى هي مش
+
+305
+00:24:36,800 --> 00:24:40,660
+auto agglutination لو طلعت negative يبقى هوالـ
+
+306
+00:24:40,660 --> 00:24:44,460
+issue اللي صار auto agglutination والنتيجة هي
+
+307
+00:24:44,460 --> 00:24:48,800
+negative مش positive يبقى هذا بالنسبة لـ slide
+
+308
+00:24:48,800 --> 00:24:49,280
+method
+
+309
+00:25:06,200 --> 00:25:10,400
+طبعاً بنعمل بعد ما أحنا عملنا اللي هو drop of
+
+310
+00:25:10,400 --> 00:25:12,900
+rabbit plasma مع touch of بكتيريا وحطينا negative
+
+311
+00:25:12,900 --> 00:25:18,580
+control بقولي بتعملي mix بالrabbit
+
+312
+00:25:18,580 --> 00:25:22,540
+plasma أو بالكمان بالwater أو ال normal saliva
+
+313
+00:25:22,540 --> 00:25:27,180
+يعني اللي هو negative control بعدين بشوف النتيجة
+
+314
+00:25:27,180 --> 00:25:31,540
+بقولي
+
+315
+00:25:31,540 --> 00:25:35,470
+observefor clumping in the coagulase plasma and
+
+316
+00:25:35,470 --> 00:25:39,430
+homogenous suspension in the control at 20 to 30
+
+317
+00:25:39,430 --> 00:25:43,350
+seconds بقوللي بلاحظ ال clumping اللي موجود فيه ال
+
+318
+00:25:43,350 --> 00:25:46,690
+coagulase plasma ال drop اللي حطيتها من البلازما و
+
+319
+00:25:46,690 --> 00:25:50,230
+ال drop اللي حطيته في ال control هنا في عندي
+
+320
+00:25:50,230 --> 00:25:53,750
+ملاحظات clump that will not mix uniformly into
+
+321
+00:25:53,750 --> 00:25:57,510
+coagulase plasma indicate a positive test بقوللي
+
+322
+00:25:57,970 --> 00:26:01,290
+الـ Positive result بيقول في عيني بيكون Clumping
+
+323
+00:26:01,290 --> 00:26:06,230
+تجمع من اللي فيه بيكون عيني حبيبات Clots أجلوتناشن
+
+324
+00:26:06,230 --> 00:26:09,990
+زي فصائل الدم لكن بالنسبة للـ Negative result
+
+325
+00:26:09,990 --> 00:26:12,770
+بيكون في عيني Uniform suspension أو Smooth
+
+326
+00:26:12,770 --> 00:26:16,230
+suspension بيكون ال suspension كتير ناعم مافي أي
+
+327
+00:26:16,230 --> 00:26:20,250
+Clot تمام هذه النتيجة بتكون Negative result يبقى
+
+328
+00:26:20,250 --> 00:26:23,090
+الـ Positive result بيكون عيني Clumping تجمع لكن
+
+329
+00:26:23,090 --> 00:26:27,390
+الـ Negative Uniform suspension Clumping in both
+
+330
+00:26:27,390 --> 00:26:30,750
+testsindicate that the organism autoagglutination
+
+331
+00:26:30,750 --> 00:26:34,670
+at is at is unsuitable for the slide coagulast
+
+332
+00:26:34,670 --> 00:26:40,250
+test قلنا لو أنا عملت الفحص و طلعت في ال sample و
+
+333
+00:26:40,250 --> 00:26:43,470
+في ال control كان عندي positive positive هذا بدل
+
+334
+00:26:43,470 --> 00:26:46,870
+عن انه عندي في autoagglutination ال
+
+335
+00:26:46,870 --> 00:26:50,250
+autoagglutination مش مناسب لل slide coagulast test
+
+336
+00:26:50,250 --> 00:26:53,850
+لازم أأكد الفحص بال tube method لأنه هي ال
+
+337
+00:26:53,850 --> 00:26:58,000
+confirmatorywhen the autoagglutination is observed
+
+338
+00:26:58,000 --> 00:27:00,280
+واللي بقولنا الـ tube method قلنا لازم أني أنا
+
+339
+00:27:00,280 --> 00:27:04,520
+أشتغلها بدل من ال slide method لأن قلنا ال tube
+
+340
+00:27:04,520 --> 00:27:07,180
+method هو ال confirmatory
+
+341
+00:27:16,790 --> 00:27:19,790
+procedure of a tube method خلصنا من ال slide
+
+342
+00:27:19,790 --> 00:27:22,470
+method وعرفنا كيف بنشتغلها وقولنا هي screening
+
+343
+00:27:22,470 --> 00:27:26,890
+method هلأ هنتكلم على ال confirmatory method اللي
+
+344
+00:27:26,890 --> 00:27:31,450
+هو ال tube method اللي بيكشف عن ال free and bound
+
+345
+00:27:31,450 --> 00:27:35,210
+عشان هي أنا قولت يعتبر confirmatory لأنه بيكشف عن
+
+346
+00:27:35,210 --> 00:27:40,230
+ال freeوالـ Bound ما بيكشف عن في حال كان فيني Auto
+
+347
+00:27:40,230 --> 00:27:44,870
+-agglutination برضه الـ tube method أنا بستخدمها
+
+348
+00:27:44,870 --> 00:27:47,330
+بدل من ال slide method فبالتالي هو يعتبر
+
+349
+00:27:47,330 --> 00:27:51,970
+confirmatory لكن ال slide فقط بتكشفلي عن ال bound
+
+350
+00:27:51,970 --> 00:27:57,560
+ال bound coagulaseأي فحص كيميائي أول جملة بقول
+
+351
+00:27:57,560 --> 00:28:00,680
+using a culture that is less than 24 hours old
+
+352
+00:28:00,680 --> 00:28:04,000
+بقولنا أي فحص كيميائي لازم ال culture تكون fresh
+
+353
+00:28:04,000 --> 00:28:09,580
+لأن ال enzyme activity بقل و بقول بتاخد من مزرعة
+
+354
+00:28:09,580 --> 00:28:14,470
+أو من culture a garden inhibitory agariplateNon
+
+355
+00:28:14,470 --> 00:28:19,030
+-inhibitory يعني أنه أخد الكولوني من أجار non
+
+356
+00:28:19,030 --> 00:28:22,670
+-selective media أفضل أني أنا أشتغل من general
+
+357
+00:28:22,670 --> 00:28:25,970
+media لأن الـ selective media فيها inhibitor هذه
+
+358
+00:28:25,970 --> 00:28:30,830
+الـinhibitor ممكن تأثر على الإنزايم أشهر الطريقة
+
+359
+00:28:30,830 --> 00:28:34,910
+بقولنا أنه بتجيب 2 ل4 كولونيز من البكتيريا اللي
+
+360
+00:28:34,910 --> 00:28:40,720
+بدك تكشف عندها الإنزايم موجود أو لأوبتجيبيهم هاي و
+
+361
+00:28:40,720 --> 00:28:44,520
+بتحطيهم في one ml of rabbit plasma وبتعملي لهم
+
+362
+00:28:44,520 --> 00:28:49,800
+incubation لمدة أربع ساعات على درجة حرارة سبعة و
+
+363
+00:28:49,800 --> 00:28:55,420
+تلاتين خلال الأربع ساعات كل شوية بطلع و بحرك بلاحظ
+
+364
+00:28:55,420 --> 00:28:58,360
+إذا اتكوّن اتجلطة أو لأ أكيف اتكوّن اتجلطة فيه
+
+365
+00:28:58,360 --> 00:29:04,320
+بيكون كلوت زي حبيبات في السائل أو ممكن نلاحظ إن
+
+366
+00:29:04,320 --> 00:29:08,160
+السائل بدأ يجمد فبالتالي هاي النتيجة ال positive
+
+367
+00:29:08,870 --> 00:29:31,830
+يبقى الـ tube method الـ
+
+368
+00:29:31,830 --> 00:29:34,330
+tube method بجيب اتنين
+
+369
+00:29:59,820 --> 00:30:10,280
+of bacteria زائد 1
+
+370
+00:30:10,280 --> 00:30:13,640
+ml of rabbit plasma
+
+371
+00:30:28,250 --> 00:30:44,630
+بعملهم incubation على
+
+372
+00:30:44,630 --> 00:30:51,770
+درجة حرارة 37 درجة سليزيا لمدة 4 hour
+
+373
+00:31:01,560 --> 00:31:06,300
+في الـ Incubator 37 درجة سليسيس لمدة 4 ساعات وبشوف
+
+374
+00:31:06,300 --> 00:31:11,500
+النتيجة قولنا خلال 4 ساعات بحاول أني انا أشيك على
+
+375
+00:31:11,500 --> 00:31:15,840
+ال tube بشوف اذا في عندي اتكون إقلوت ولا لأ بشوف
+
+376
+00:31:15,840 --> 00:31:20,000
+إذا السائل جمد أو لأ هلأ لو كانت النتيجة positive
+
+377
+00:31:20,000 --> 00:31:27,220
+كان في عندي إقلوت
+
+378
+00:31:31,890 --> 00:31:36,870
+أو لاحظت ان السائل جمد فتمام يبقى بحكي انه هي
+
+379
+00:31:36,870 --> 00:31:41,190
+النتيجة positive في عندي انزيم الـ coagulase لكن
+
+380
+00:31:41,190 --> 00:31:51,210
+لو ماكان في عندي اي clot وكانت نوف ال clot السائل
+
+381
+00:31:51,210 --> 00:31:55,310
+دلوا زي ما هو بقولي النتيجة ما بسجلها negative
+
+382
+00:31:55,310 --> 00:32:00,830
+تمام ما منسجل النتيجة negative بقولناباروح انا
+
+383
+00:32:00,830 --> 00:32:06,070
+بعمل incubation بكمل ال incubation ل 24 ساعة احنا
+
+384
+00:32:06,070 --> 00:32:09,390
+عملنا 4 ساعات يبقى ضال في عندي 20 ساعة بعمل
+
+385
+00:32:09,390 --> 00:32:13,570
+incubation لمدة 20 ساعة لكن على ال room
+
+386
+00:32:13,570 --> 00:32:17,210
+temperature يبقى لو ما كان عندى تكون ال cloth
+
+387
+00:32:17,210 --> 00:32:21,030
+مابحكي negative بعملها incubation هاي النتيجة يبقى
+
+388
+00:32:21,030 --> 00:32:24,390
+بعملها incubation
+
+389
+00:32:34,180 --> 00:32:40,660
+at room temperature لمدة
+
+390
+00:32:40,660 --> 00:32:46,120
+20 ساعة و
+
+391
+00:32:46,120 --> 00:32:50,940
+اللي هو of total 24 hours احنا عملنا 4 ساعات يبقى
+
+392
+00:32:50,940 --> 00:32:55,240
+بدي عشان اكمل لل 24 بحطها 24 ساعة لكن على room
+
+393
+00:32:55,240 --> 00:32:59,680
+temperature بعد بعد ما تخلص ال 20 ساعة يعني ال
+
+394
+00:32:59,680 --> 00:33:04,980
+total 24 ساعة لوكان في عندي no clot يبقى خلاص
+
+395
+00:33:04,980 --> 00:33:08,800
+بعتمد النتيجة negative لكن لو اتغير وصار في عندي
+
+396
+00:33:08,800 --> 00:33:12,980
+clot يبقى هي positive يبقى هنا في المرحلة هاي انا
+
+397
+00:33:12,980 --> 00:33:17,840
+بحكي انه لو اتكون clot يبقى هي positive
+
+398
+00:33:26,710 --> 00:33:33,330
+لكن هان خلاص بعد 24 ساعة بتكون كافية انه انا احكي
+
+399
+00:33:33,330 --> 00:33:40,410
+انه هي negative نو اكلوت او السائل ما جمد يبقى
+
+400
+00:33:40,410 --> 00:33:45,030
+باعتبر انه نتيجة هان negative يبقى بعد 24 ساعة
+
+401
+00:33:45,030 --> 00:33:49,370
+بأكد النتيجة ال negative هذا بالنسبة لليتيوب
+
+402
+00:33:49,370 --> 00:33:52,890
+method هيك الطريقة هيك الشغل لليتيوب method
+
+403
+00:34:00,080 --> 00:34:03,320
+بقولنا negative test بقولنا لو الفحص كان negative
+
+404
+00:34:03,320 --> 00:34:06,320
+بعد أربع ساعات بعمله على ال room temperature زي ما
+
+405
+00:34:06,320 --> 00:34:10,500
+قولنا عشان بنكمل ل total of 24 ساعة يعني بنحط
+
+406
+00:34:10,500 --> 00:34:14,820
+عشرين ساعة في ال room temperature كيف بقرأ النتيجة
+
+407
+00:34:14,820 --> 00:34:17,880
+بقولنا بتميلي let you و بتلاحظي اللي هو clotting
+
+408
+00:34:17,880 --> 00:34:18,720
+of plasma
+
+409
+00:34:28,300 --> 00:34:31,660
+بنقرأ النتائج بقولّي إنه الـ Positive test for
+
+410
+00:34:31,660 --> 00:34:34,680
+Coagulase production result بكون في عندي Clotting
+
+411
+00:34:34,680 --> 00:34:38,840
+of the rabbit plasma لو في عندي إشي بسيط من لو
+
+412
+00:34:38,840 --> 00:34:41,640
+كانت ال Clot حتى لو بسيطة أنا بعتبرها Positive
+
+413
+00:34:41,640 --> 00:34:46,860
+result بقولّي النتيجة بكتبها من Range 0 to plus 4
+
+414
+00:34:46,860 --> 00:34:51,670
+Zero معناه إنهنوع Geolase Activity يعني Zero بتكون
+
+415
+00:34:51,670 --> 00:34:56,730
+Negative البلازمة بيبقى Liquid بيضلوا البلازمة
+
+416
+00:34:56,730 --> 00:35:01,590
+سائل مافي عندى اي Clot لكن بالنسبة لـ Plus One الـ
+
+417
+00:35:01,590 --> 00:35:05,630
+Plus One انا بعتبرها اللى هي weak positive بيكون
+
+418
+00:35:05,630 --> 00:35:10,630
+فيه عندى Clot لكن خفيف السائل مش جامد كتير زي الـ
+
+419
+00:35:10,630 --> 00:35:13,310
+Positive Four بالنسبة لـ Plus Two و Plus Three
+
+420
+00:35:13,310 --> 00:35:16,610
+بنعتبرها Intermediate Positive بيكون فيه عندى Clot
+
+421
+00:35:16,930 --> 00:35:20,130
+لكن مش زي الـ plus four الـ plus four بيكون
+
+422
+00:35:20,130 --> 00:35:24,230
+البلازما صار عندى جامد بشكل كامل طب أنا مهادة
+
+423
+00:35:24,230 --> 00:35:27,410
+بعتبرها strong positive الـ plus four يبقى strong
+
+424
+00:35:27,410 --> 00:35:30,390
+positive يبقى zero تعتبر negative لكن الـ plus one
+
+425
+00:35:30,390 --> 00:35:33,650
+plus two plus three plus four تعتبر positive لكن
+
+426
+00:35:33,650 --> 00:35:38,870
+ال positive بتختلف plus four تعتبر strong positive
+
+427
+00:35:38,870 --> 00:35:42,610
+لكن plus one تعتبر weak positive احنا قولنا اي
+
+428
+00:35:42,610 --> 00:35:47,660
+درجة من لكلت انا بعتبرها positive testكل احنا قلنا
+
+429
+00:35:47,660 --> 00:35:53,540
+اي نتيجة Zero ماكان في عندي ضلوا ال plasma سائل
+
+430
+00:35:53,540 --> 00:35:56,840
+بعد 4 ساعات بحطها على ال room temperature و بكمل
+
+431
+00:35:56,840 --> 00:35:59,860
+incubation لمدة 24 ساعة
+
+432
+00:36:03,310 --> 00:36:06,230
+في انديهان ملاحظات مهمة بقول لي when the slide
+
+433
+00:36:06,230 --> 00:36:09,490
+test is employed all negative slide reaction must
+
+434
+00:36:09,490 --> 00:36:13,490
+be confirmed by the tube test قولنا لو اشتغلت ال
+
+435
+00:36:13,490 --> 00:36:18,290
+slide method وطلعت negative لازم انا اشتغل ال tube
+
+436
+00:36:18,290 --> 00:36:21,430
+method لأن احنا قولنا ال tube يعتبر confirmatory
+
+437
+00:36:21,430 --> 00:36:26,250
+test هو بيكشفلي على ال free و ال bound لأن ال
+
+438
+00:36:26,250 --> 00:36:30,090
+slide test لو انت اشتغلتيها وطلعت negative هي
+
+439
+00:36:30,090 --> 00:36:35,530
+بتكون negative لمن؟ لل boundلكن أنا بدي أكشف عن
+
+440
+00:36:35,530 --> 00:36:39,470
+الـ Free Bluetooth Method فبالتالي بستخدم
+
+441
+00:36:39,470 --> 00:36:43,090
+Bluetooth Method عشان أشوف هل عندها Free ولا لأ لو
+
+442
+00:36:43,090 --> 00:36:46,270
+طلعت Positive بـ Bluetooth Method يبقى عندها Free
+
+443
+00:36:46,270 --> 00:36:49,430
+Coagulase لو طلعت Negative بـ Bluetooth Method بعد
+
+444
+00:36:49,430 --> 00:36:52,150
+ما عملتها بالـ Slide وطلعت Negative يبقى ما عندها
+
+445
+00:36:52,150 --> 00:36:57,900
+لا Free ولا Boundالتكنيكية الـ slide agglutination
+
+446
+00:36:57,900 --> 00:37:04,180
+قد تؤدي إلى نتيجة فالس بوزيتف بقول لي أن الـ slide
+
+447
+00:37:04,180 --> 00:37:08,620
+method ممكن تعطيني نتيجة فالس بوزيتف لماذا؟ لأن
+
+448
+00:37:08,620 --> 00:37:12,520
+بعض
+
+449
+00:37:12,520 --> 00:37:16,580
+الأنواع من البكتيريا تنتج نتيجة فالس بوزيتف الـ
+
+450
+00:37:16,580 --> 00:37:19,460
+clumping factor نفسه هو معناه أنها بتعمل auto
+
+451
+00:37:19,460 --> 00:37:23,580
+agglutination يبقى بتعطيني نتيجة فالس بوزيتف في
+
+452
+00:37:23,580 --> 00:37:29,110
+الـ slideعشان هيك لازم انا اعيد النتيجة بالـ tube
+
+453
+00:37:29,110 --> 00:37:32,330
+method قولنا الـ tube method هو يعتبر confirmatory
+
+454
+00:37:32,330 --> 00:37:36,310
+و لازم كمان انا اعمل negative control لو طلعت معي
+
+455
+00:37:36,310 --> 00:37:39,270
+positive معناه ان في عندي لو طلع negative control
+
+456
+00:37:39,270 --> 00:37:42,750
+positive معناه في عندي autoagglutination لازم اعيد
+
+457
+00:37:42,750 --> 00:37:46,720
+الفحص بالـ tube method ال confirmatoryلو بالـ tube
+
+458
+00:37:46,720 --> 00:37:50,840
+method طلع positive يبقى هي بتكون coagulase
+
+459
+00:37:50,840 --> 00:37:54,400
+positive لكن لو طلع negative بتكون هي coagulase
+
+460
+00:37:54,400 --> 00:37:57,680
+negative ال agglutination اللي دهر هو نتيجة ال
+
+461
+00:37:57,680 --> 00:38:01,800
+autoagglutination فقط spontaneous agglutination
+
+462
+00:38:01,800 --> 00:38:04,240
+may occur when rough culture are used بقوللي
+
+463
+00:38:04,240 --> 00:38:06,640
+spontaneous agglutination هو نفس ال
+
+464
+00:38:06,640 --> 00:38:10,800
+autoagglutinationبلاحظه خصوصًا لو أخدت البكتيريا
+
+465
+00:38:10,800 --> 00:38:15,500
+من لو كانت الـ colony non-capsulated رف معناه إنه
+
+466
+00:38:15,500 --> 00:38:21,160
+non-capsulated بكتيريا ليش أنا بلاحظ من الـ auto
+
+467
+00:38:21,160 --> 00:38:24,020
+-agglutination بيصير في الـ non-capsulated بكتيريا
+
+468
+00:38:24,020 --> 00:38:29,140
+أحنا قلنا الكوسول يعتبر أو يستخدم ك protection
+
+469
+00:38:29,140 --> 00:38:33,570
+fromالـ Phagocytosis والـ Coagulase Enzyme نفس
+
+470
+00:38:33,570 --> 00:38:37,070
+الفكرة قلنا بيحول الفيبرينوجين لفيبرين الفيبرين
+
+471
+00:38:37,070 --> 00:38:40,990
+لكلوت البكتيريا بتتخبى جوا هذه الفيبرين فبالتالي
+
+472
+00:38:40,990 --> 00:38:44,750
+بتحميها من الـ Phagocytosis يبقى لو ما عندها
+
+473
+00:38:44,750 --> 00:38:47,970
+capsule أكيد هتلجأ للـ Coagulase أو لو ما عندها
+
+474
+00:38:47,970 --> 00:38:51,910
+Coagulase أكيد هتلجأ للـ Autoagglutination عشان
+
+475
+00:38:51,910 --> 00:38:55,610
+يصير في عندي عشان تحميها من الـ Phagocytosis
+
+476
+00:38:55,610 --> 00:38:59,480
+فبنلاحظ أن الـ Spontaneous Agglutinationبيكون فيه
+
+477
+00:38:59,480 --> 00:39:03,580
+الـ non-capsulated bacteria بقولي آخر إشي أنه ال
+
+478
+00:39:03,580 --> 00:39:07,140
+tube method أفضل من ال slide method قلنا لأنه ال
+
+479
+00:39:07,140 --> 00:39:10,820
+tube method بيكشف عن ال free و ال bound لكن ال
+
+480
+00:39:10,820 --> 00:39:15,280
+slide بيكشف عن ال bound فقط ال slide بيصير فيه
+
+481
+00:39:15,280 --> 00:39:18,340
+اندياط و agglutination لازم أني أنا أعمل ال tube
+
+482
+00:39:18,340 --> 00:39:23,310
+method عشان أتأكد ال slide test should be readvery
+
+483
+00:39:23,310 --> 00:39:26,050
+quickly بقولّي لو انت اشتغلت بـ slide method لازم
+
+484
+00:39:26,050 --> 00:39:29,630
+تقرأيها بسرعة false positive result ممكن انه يصير
+
+485
+00:39:29,630 --> 00:39:34,170
+ال test should then perform with organism detected
+
+486
+00:39:34,170 --> 00:39:37,590
+from high salt media such as manitol salt agar
+
+487
+00:39:37,590 --> 00:39:42,510
+احنا قلنا انه اي فحص كيميائي لازم ان انا اشتغله من
+
+488
+00:39:42,510 --> 00:39:45,390
+general media ال manitol salt agar تعتبر selective
+
+489
+00:39:45,390 --> 00:39:48,590
+media فيها inhibitor اللي هو ال salt بقول ال salt
+
+490
+00:39:48,590 --> 00:39:53,550
+ممكن انه يؤثر على الانزيمات فبالتاليتعطيني False
+
+491
+00:39:53,550 --> 00:39:56,950
+Positive Result هذه النتيجة إذا ما نلاحظ الـ
+
+492
+00:39:56,950 --> 00:39:59,030
+Staphylococcus aureus قولنا هي Positive للـ
+
+493
+00:39:59,030 --> 00:40:01,650
+Coagulase في عندى حبيبات، في عندى Clots لكن
+
+494
+00:40:01,650 --> 00:40:05,090
+Staphylococcus epidermidis هي Negative للـ
+
+495
+00:40:05,090 --> 00:40:07,850
+Coagulase منلاحظ أنه في عندى Smooth Suspension
+
+496
+00:40:07,850 --> 00:40:10,990
+Uniformة في عندى أي حبيبات هذا بالنسبة للـ Slide
+
+497
+00:40:10,990 --> 00:40:14,490
+Method لكن للـ Tube Method الـ Coagulase Positive
+
+498
+00:40:14,490 --> 00:40:18,620
+منلاحظ أن الـ Plasma جمدتلكن الـ Coagulase
+
+499
+00:40:18,620 --> 00:40:22,200
+Negative أنه مافي عندي Clot و البلازمه ضاله سائل
+
+500
+00:40:22,200 --> 00:40:26,480
+حيث أننا خلصنا الـ Coagulase Test و Catalase Test
+
+501
+00:40:26,480 --> 00:40:31,180
+و حيث أننا خلصنا الـ Chapter لليوم والسلام عليكم
+
+502
+00:40:31,180 --> 00:40:32,560
+ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/InTN5cUZCVI_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/InTN5cUZCVI_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..a02ea41558b752571c34a137337f08c7d796e1f0
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/InTN5cUZCVI_postprocess.srt
@@ -0,0 +1,1252 @@
+1
+00:00:02,530 --> 00:00:06,650
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:06,650 --> 00:00:11,090
+جميعا في هذا اللقاء هنستكمل في موضوع الستان في
+
+3
+00:00:11,090 --> 00:00:15,470
+المحاضرة السابقة قسمنا الاستان حسب ال function ل
+
+4
+00:00:15,470 --> 00:00:19,770
+symbol استان differential استان و special استان
+
+5
+00:00:19,770 --> 00:00:23,310
+اليوم ان شاء الله موضوعنا هيكون عن ال differential
+
+6
+00:00:23,310 --> 00:00:27,550
+استان اتفقنا ان ال differential استان هي صبغة
+
+7
+00:00:27,550 --> 00:00:32,140
+تستخدم لتقسيم البكتيريا لمجموعاتDivide bacteria
+
+8
+00:00:32,140 --> 00:00:36,820
+into large groups من الأمثلة على الـDifferential
+
+9
+00:00:36,820 --> 00:00:42,860
+Stain لجرام Stain والAcid Fast Stain بالنسبة لجرام
+
+10
+00:00:42,860 --> 00:00:47,640
+Stain قسمت البكتيريا لجرام Positive بكتيريا وجرام
+
+11
+00:00:47,640 --> 00:00:51,900
+Negative بكتيريا تم تقسيم البكتيريا فيه لجرام
+
+12
+00:00:51,900 --> 00:00:56,620
+Stain على حسب الـcell wall تبع البكتيريا طيب من أش
+
+13
+00:00:56,620 --> 00:01:01,020
+بتكون ال cell wall؟الـ Cell Wall بتكون من بيبتيدو
+
+14
+00:01:01,020 --> 00:01:05,600
+إجلايكان بدنا نتعرف على البيبتيدو إجلايكان
+
+15
+00:01:05,600 --> 00:01:12,800
+structure البيبتيدو
+
+16
+00:01:12,800 --> 00:01:17,160
+إجلايكان بتكون من alternative unit من وحدات متكررة
+
+17
+00:01:17,160 --> 00:01:20,580
+من الـ unacetylamuramic acid و الـ
+
+18
+00:01:20,580 --> 00:01:25,040
+unacetylglucosamine بتتكرر بقولنا لو كان أنا عندي
+
+19
+00:01:25,300 --> 00:01:29,940
+طبقة واحدة من الـ peptidoglycan بتكون البكتيريا
+
+20
+00:01:29,940 --> 00:01:34,520
+إجرام نيجاتيف بكتيريا لكن لو كان أنا عندي عدة
+
+21
+00:01:34,520 --> 00:01:39,300
+طبقات من الـ peptidoglycan بتكون البكتيريا إجرام
+
+22
+00:01:39,300 --> 00:01:44,500
+positive بكتيريا طيب عشان يكون عندي عدة طبقات لازم
+
+23
+00:01:44,500 --> 00:01:48,840
+يكون فيه روابط بين هاي الطبقات بقولنا إن الـ
+
+24
+00:01:48,840 --> 00:01:53,770
+unacetylimeramic acid بيطلع منهاأربع أمينو أسد
+
+25
+00:01:53,770 --> 00:01:57,470
+أربع أحماض أمينية زي ما أحنا شايفين بالصورة
+
+26
+00:01:57,470 --> 00:02:05,010
+مربوطين ب cross-link بـ peptide bond لو
+
+27
+00:02:05,010 --> 00:02:08,670
+بدي أعمل مقارنة بين الـ Gram positive bacteria و
+
+28
+00:02:08,670 --> 00:02:12,570
+Gram negative bacteria أول وجه مقارنة بـ Peptido
+
+29
+00:02:12,570 --> 00:02:15,630
+glycan layer اتفقنا لـ Gram positive bacteria
+
+30
+00:02:15,630 --> 00:02:19,600
+Multilayer of Peptido glycanلكن الـ Gram-negative
+
+31
+00:02:19,600 --> 00:02:23,380
+bacteria one layer of peptido-pig lichen تاني
+
+32
+00:02:23,380 --> 00:02:27,160
+الشيء الـ lipid and lipoprotein content الـ Gram
+
+33
+00:02:27,160 --> 00:02:30,980
+-positive bacteria low concentration of lipid لكن
+
+34
+00:02:30,980 --> 00:02:34,120
+الـ Gram-negative bacteria high concentration خاصة
+
+35
+00:02:34,120 --> 00:02:37,360
+في الـ outer membrane الـ outer membrane of the
+
+36
+00:02:37,360 --> 00:02:41,200
+cell wall فقط موجود في الـ Gram-negative bacteria
+
+37
+00:02:41,200 --> 00:02:44,600
+ومش موجود في الـ Gram-positive bacteria كذلك الـ
+
+38
+00:02:44,600 --> 00:02:47,540
+periplasmic membraneموجود في الـ Gram Negative
+
+39
+00:02:47,540 --> 00:02:50,560
+بكتيريا ومش موجود في الـ Gram Positive بكتيريا
+
+40
+00:02:50,560 --> 00:02:54,120
+البوليساكاريد السكريات في الـ Gram Positive
+
+41
+00:02:54,120 --> 00:02:58,180
+بكتيريا في عندي بوليساكاريد زي الـ Thycoic Acid
+
+42
+00:02:58,180 --> 00:03:01,500
+اللي بيطلع من الـ cell wall والـ Lipo Thycoic Acid
+
+43
+00:03:01,500 --> 00:03:04,760
+اللي بيطلع من ال cell membrane على عكس ال Gram
+
+44
+00:03:04,760 --> 00:03:08,180
+Negative بكتيريا ماعندي بوليساكاريد لكن في عندي
+
+45
+00:03:08,180 --> 00:03:11,940
+Lipopolysaccharide موجود في ال outer membrane
+
+46
+00:03:12,730 --> 00:03:16,610
+الليبو بوليساكريد من الليبو اتفقنا انه لجرام
+
+47
+00:03:16,610 --> 00:03:21,150
+negative بكتيريا high concentration of لبد خاصة في
+
+48
+00:03:21,150 --> 00:03:25,990
+ال outer membrane اخر وجه مقارنة التوكسين لجرام
+
+49
+00:03:25,990 --> 00:03:30,930
+positive بكتيريا بتفرز اكزو توكسين heat labile
+
+50
+00:03:30,930 --> 00:03:36,130
+toxin بيتأثر بدرجة الحرارة produced from some
+
+51
+00:03:36,130 --> 00:03:40,920
+species of living bacteriaLiving bacteria لأن
+
+52
+00:03:40,920 --> 00:03:46,200
+الاندوتوكسين اتفقنا بتفرز البكتيريا لما تموت from
+
+53
+00:03:46,200 --> 00:03:51,880
+dead bacteria بالنسبة لالإكزوتوكسين ممكن تفرزه
+
+54
+00:03:51,880 --> 00:03:55,420
+some species of gram negative bacteria لكن
+
+55
+00:03:55,420 --> 00:04:01,480
+الاندوتوكسين هو heat stable toxin produced only by
+
+56
+00:04:01,480 --> 00:04:05,000
+gram negative bacteria فقط بتفرزه لgram negative
+
+57
+00:04:05,000 --> 00:04:09,790
+bacteriaلأن الـ Endotoxin هو عبارة عن الـ Libid-A
+
+58
+00:04:09,790 --> 00:04:14,010
+اللي موجود في الـ Outer Membrane اللي موجود فقط في
+
+59
+00:04:14,010 --> 00:04:17,290
+الـ Gram Negative Bacteria الـ Outer Membrane
+
+60
+00:04:17,290 --> 00:04:23,270
+بيحتوي على Lipopolysaccharide اللي بتكون من Core
+
+61
+00:04:23,270 --> 00:04:27,030
+من Libid-A اللي هو عبارة عن الـ Endotoxin و بتكون
+
+62
+00:04:27,030 --> 00:04:31,370
+أيضا من الـ O Antigen الـ O Antigen هذا بيعمل
+
+63
+00:04:31,370 --> 00:04:37,410
+Antigenic Variation بيعمل تفراديعني لو دخل ميكروب
+
+64
+00:04:37,410 --> 00:04:41,990
+الجسم لو دخلت بكتيريا الجسم بيغير الانتجن بالتالي
+
+65
+00:04:41,990 --> 00:04:48,570
+ما بيتعرف على جهاز المنار بكتيريا
+
+66
+00:04:48,570 --> 00:04:52,310
+الcell wall بنلاحظ فيه الصورة في جرام بوزة
+
+67
+00:04:52,310 --> 00:04:56,610
+البكتيريا والجرام نكت البكتيريا بتحتوي على plasma
+
+68
+00:04:56,610 --> 00:05:01,650
+membrane لكن الفرق في ال cell wall في جرام بوزة
+
+69
+00:05:01,650 --> 00:05:05,330
+البكتيريا اللي بيبتيده أجلاي كان thickلكن في الـ
+
+70
+00:05:05,330 --> 00:05:08,750
+Gram-Negative اللي بيبتدوا as like and thin كذلك
+
+71
+00:05:08,750 --> 00:05:11,850
+أيضًا بنلاحظ أنه أنا في عندي في الـ Gram-Negative
+
+72
+00:05:11,850 --> 00:05:15,790
+بكتيريا outer membrane of cell wall مش موجود في
+
+73
+00:05:15,790 --> 00:05:21,170
+الـ Gram-Positive بكتيريا هذه
+
+74
+00:05:21,170 --> 00:05:25,130
+صورة توضحية للـ Gram-Positive بكتيريا في عندي cell
+
+75
+00:05:25,130 --> 00:05:29,270
+membrane بيبتدوا as like and layer، thick بالنسبة
+
+76
+00:05:29,270 --> 00:05:32,510
+للـ Polysaccharide في عندي Polysaccharide في ال
+
+77
+00:05:32,510 --> 00:05:36,180
+Gram-Positive بكتيرياالثيكوك أسد موجود في الـ cell
+
+78
+00:05:36,180 --> 00:05:39,940
+wall والليبوثيكوك أسد بيطلع من الـ cell membrane
+
+79
+00:05:39,940 --> 00:05:45,720
+بالأ لجرام نيجاتيف بكتيريا في cell membrane زي
+
+80
+00:05:45,720 --> 00:05:49,220
+لجرام positive بكتيريا ببتيدوز like هال layer of
+
+81
+00:05:49,220 --> 00:05:53,380
+cell wall 10 تحتوي على outer membrane of cell wall
+
+82
+00:05:53,380 --> 00:05:58,300
+اللي أحد مقويناته الليبو بولي ساكرايت اللي بيتكون
+
+83
+00:05:58,300 --> 00:06:03,300
+من ال lipid A اللي قولنا هو ال endotoxinأيضاً في
+
+84
+00:06:03,300 --> 00:06:06,960
+إن بيري إبلازمك اسباس في الـ Gram-negative بكتيريا
+
+85
+00:06:06,960 --> 00:06:13,200
+مش موجود في الـ Gram-positive بكتيريا كيف
+
+86
+00:06:13,200 --> 00:06:17,160
+تم اكتشاف الـ Gram-stain؟ في طبيب دنماركي اسمه
+
+87
+00:06:17,160 --> 00:06:21,240
+Christian Igram كان بيشتغل بالمختبر جاب بعض أنواع
+
+88
+00:06:21,240 --> 00:06:25,340
+البكتيريا جاب بكتيريا X وبكتيريا Y وصبغها
+
+89
+00:06:25,340 --> 00:06:29,000
+بـCrystal Violet الـCrystal Violet هي عبارة عن
+
+90
+00:06:29,000 --> 00:06:33,380
+بازكستان بتحمل شحنة موجبةهتصبغ البكتيريا سالبة
+
+91
+00:06:33,380 --> 00:06:38,140
+الشحنة انصبغت كل البكتيريا سواء كانت X أو Y بي الـ
+
+92
+00:06:38,140 --> 00:06:42,600
+Crystal Violet وأخدت اللون الـ Violet بعد كده أضاف
+
+93
+00:06:42,600 --> 00:06:47,220
+الـ Mordant المثبت الـ Iodine ثبت اللون داخل
+
+94
+00:06:47,220 --> 00:06:51,380
+البكتيريا تالت خطوة أضاف الـ Decolorizer مزيل
+
+95
+00:06:51,380 --> 00:06:56,940
+اللون مدة معينة عبارة عن كحول لاحظ أن اللون راح من
+
+96
+00:06:56,940 --> 00:07:02,000
+البكتيريا Yلكن بكتيريا X ضلت محتفظة باللون الفيولت
+
+97
+00:07:02,000 --> 00:07:06,880
+بعد كده أضاف صبغة تانية Basic Stain الصفرانين
+
+98
+00:07:06,880 --> 00:07:10,460
+البكتيريا اللي راح اللون منها أخدت الصبغة high
+
+99
+00:07:10,460 --> 00:07:13,580
+والبكتيريا اللي ما راح اللون منها ما أخدت الصبغة
+
+100
+00:07:13,580 --> 00:07:17,840
+ضلت محتفظة بالصبغة الأولى لcrystal violet ثم
+
+101
+00:07:17,840 --> 00:07:22,480
+البكتيريا اللي ضلت محتفظة باللون وما نزل وأخدت
+
+102
+00:07:22,480 --> 00:07:26,130
+اللون الفيولت بال gram positive bacteriaواللي أخدت
+
+103
+00:07:26,130 --> 00:07:29,670
+اللون الـ Pink وراح اللون منها بالـ Gram Negative
+
+104
+00:07:29,670 --> 00:07:34,510
+Bacteria السؤال، ليش البكتيريا الـ Gram Positive
+
+105
+00:07:34,510 --> 00:07:38,690
+Bacteria دلت محتفظة باللون الـ Violet؟ بناء على
+
+106
+00:07:38,690 --> 00:07:42,770
+المقارنة اللي عملناها ممكن نتفسر هذه النتيجة الـ
+
+107
+00:07:42,770 --> 00:07:46,230
+Gram Negative Bacteria بتتكون من طبقة واحدة من الـ
+
+108
+00:07:46,230 --> 00:07:50,150
+Peptidoglycan بالتالي سهل على الـ Decolorizer
+
+109
+00:07:50,150 --> 00:07:54,070
+الكحول يدخل ويزيل اللونلكن الـ Gram-positive
+
+110
+00:07:54,070 --> 00:07:57,330
+بكتيريا بتتكون من Multi-layer من الـ Betadoglycan
+
+111
+00:07:57,330 --> 00:08:01,990
+هتظل محتفظة باللون السبب التاني قلنا إنه أنا في
+
+112
+00:08:01,990 --> 00:08:05,030
+عندي في الـ Gram-negative بكتيريا كمية عالية من
+
+113
+00:08:05,030 --> 00:08:09,390
+الدهون High Rich of Lipid و الـ Decolorizer عبارة
+
+114
+00:08:09,390 --> 00:08:13,130
+عن كحول و الكحول بدوب في الدهون فرح يزيل اللون
+
+115
+00:08:13,130 --> 00:08:18,260
+منها و لما أصبغ بصبغة تانيةاللي هي الصفرانين إش
+
+116
+00:08:18,260 --> 00:08:21,360
+طبيعي إنها تنصبغي البكتيريا اللي مش مصبوغة لكن
+
+117
+00:08:21,360 --> 00:08:28,560
+البكتيريا المصبوغة هتظل لونها زي ما هو إجرام
+
+118
+00:08:28,560 --> 00:08:32,560
+أستان، differential أستان تفقنا إن الإجرام أستان
+
+119
+00:08:32,560 --> 00:08:36,380
+هي نوع من أنواع differential أستان اللي بتقسم
+
+120
+00:08:36,380 --> 00:08:41,240
+البكتيريا لمجموعات هانزي كريستيان إجرام أدنش دكتور
+
+121
+00:08:41,240 --> 00:08:44,580
+he developed a new method to stain bacteriaلذلك
+
+122
+00:08:44,580 --> 00:08:47,720
+يمكن أن يكونوا مشهورين في مجموعات مخصصة هذه
+
+123
+00:08:47,720 --> 00:08:52,160
+الكريستيان إيجرام طبيب دنماركي طور طريقة جديدة
+
+124
+00:08:52,160 --> 00:08:56,720
+لصباغة البكتيريا الـ Gram Stain اختلط البكتيريا
+
+125
+00:08:56,720 --> 00:09:01,460
+إلى عضو كبير قسمت البكتيريا لمجموع تاني ال Gram
+
+126
+00:09:01,460 --> 00:09:05,760
+Positive و Gram Negative هذا الهدف من ال Gram
+
+127
+00:09:05,760 --> 00:09:11,400
+Stainإجرام status is important in medicine the
+
+128
+00:09:11,400 --> 00:09:14,960
+presence or absence of a cell wall will change the
+
+129
+00:09:14,960 --> 00:09:19,200
+bacterium susceptibility to some antibiotic بقولنا
+
+130
+00:09:19,200 --> 00:09:23,540
+مهم جدا أعرف هل البكتيريا إجرام positive أو إجرام
+
+131
+00:09:23,540 --> 00:09:28,100
+negative why؟ لأن وجود أو غياب ال cell wall هيغير
+
+132
+00:09:28,100 --> 00:09:33,550
+استجابة البكتيريا للمضادات الحيوية كيف؟المضادات
+
+133
+00:09:33,550 --> 00:09:37,470
+الحيوية بتأثر على البكتيريا من خلال استهدافها
+
+134
+00:09:37,470 --> 00:09:42,670
+لتراكيب داخل الخلية ممكن تستهدف السلوال ممكن
+
+135
+00:09:42,670 --> 00:09:46,930
+البلوتين ممكن الريبوسان ممكن أي مكون داخل الخلية
+
+136
+00:09:46,930 --> 00:09:50,730
+بالنسبة للسلوال لأن الـ Gram Stain باعتمد عليه
+
+137
+00:09:50,730 --> 00:09:54,550
+قولنا إن السلوال بتكون من بيبتيدوجليكان
+
+138
+00:09:54,550 --> 00:09:57,650
+البيبتيدوجليكان بتكون من وحدات من
+
+139
+00:09:57,650 --> 00:10:01,850
+الـunacetylglucosamine و الـunacetylamuramic acid
+
+140
+00:10:02,200 --> 00:10:06,100
+الـ Unacetyl Muramic Acid بيطلع منها أحماض أمينية
+
+141
+00:10:06,100 --> 00:10:10,840
+هذه الأحماض مربوطة بالـ cross-link بـ peptide bond
+
+142
+00:10:10,840 --> 00:10:15,740
+في مضادات حيوية زي الـ penicillin بتستهدف هذه
+
+143
+00:10:15,740 --> 00:10:19,600
+الروابط و بتكسرها يعني بتستهدف فقط لـ gram
+
+144
+00:10:19,600 --> 00:10:23,040
+positive bacteria لأن اتفقنا لـ gram positive
+
+145
+00:10:23,040 --> 00:10:26,220
+bacteria multilayer of peptidoglycan هي الموجود
+
+146
+00:10:26,220 --> 00:10:30,000
+فيها الروابط لكن لـ gram negative طبقة واحدة
+
+147
+00:10:30,000 --> 00:10:35,610
+مافيها أي روابطيعني البنسلل لو أعطيته لمريض مصاب
+
+148
+00:10:35,610 --> 00:10:41,030
+بالتهاب بكتيريا موجبة إجرام هيستجيب لهذا المضاد
+
+149
+00:10:41,030 --> 00:10:45,090
+لكن لو أعطيته لمريض مصاب بالتهاب بكتيريا سالبة
+
+150
+00:10:45,090 --> 00:10:50,250
+إجرام ما رح يستجيب يبقى ضروري جدا أكون عارفة هل
+
+151
+00:10:50,250 --> 00:10:54,990
+البكتيريا موجبة إجرام أو سالبة إجرام لأن استجابتها
+
+152
+00:10:54,990 --> 00:10:56,530
+للمضادات هتختلف
+
+153
+00:11:01,040 --> 00:11:04,680
+almost all bacteria are described by their gram
+
+154
+00:11:04,680 --> 00:11:08,600
+stand characteristic تقريبا كل البكتيريا بيتم
+
+155
+00:11:08,600 --> 00:11:12,980
+ثباتتها بالgram stand إذا ملاحظين ما حكى all
+
+156
+00:11:12,980 --> 00:11:17,200
+bacteria قال almost all bacteria ليش لأن في عندي
+
+157
+00:11:17,200 --> 00:11:20,860
+بكتيريا اسمها ال micro bacterium هذه البكتيريا
+
+158
+00:11:20,860 --> 00:11:25,040
+موجودة على ال cell wall تبعها waxy lipid ال waxy
+
+159
+00:11:25,040 --> 00:11:29,780
+lipidبيعمل كحاجز لمانع دخول الصبغة فبالتالي ما
+
+160
+00:11:29,780 --> 00:11:33,400
+بقدر أصبغها بالـ Simple Stain ولا بالـ Gram Stain
+
+161
+00:11:33,400 --> 00:11:38,960
+هلجأ لطريقة أخرى لصبغتها بالـ Acid Fast Stain اللي
+
+162
+00:11:38,960 --> 00:11:43,600
+هنتعرف عليها في هذه المحاضرة Based on differences
+
+163
+00:11:43,600 --> 00:11:47,780
+of cell wall structure اتفقنا أن الصبغة بتكون حسب
+
+164
+00:11:47,780 --> 00:11:53,940
+الاختلاف في تركيب ال cell wall Reagent
+
+165
+00:11:53,940 --> 00:11:59,010
+for Gram StainCrystal Violet Primer Stain هي
+
+166
+00:11:59,010 --> 00:12:02,830
+الصبغة الأولية أول صبغة بستخدمها Positive Stain
+
+167
+00:12:02,830 --> 00:12:07,150
+يعني بتحمل شح نموجبة يعني Basic Stain عشان تصبغ
+
+168
+00:12:07,150 --> 00:12:12,170
+البكتيريا الصالبة Stain Cellule Parbol بتعطي اللون
+
+169
+00:12:12,170 --> 00:12:16,650
+البربل تاني مكوّن ال Iodine ال Iodine يعتبر
+
+170
+00:12:16,650 --> 00:12:21,290
+Mordant مثبتشو الـ principle تبع الـ Iodine بقول
+
+171
+00:12:21,290 --> 00:12:25,070
+ليه؟ combines with primary stain بيرتبط مع ال
+
+172
+00:12:25,070 --> 00:12:29,710
+primary stain اللي هي ال crystal violet to form an
+
+173
+00:12:29,710 --> 00:12:34,910
+insoluble complex عشان يكون insoluble complex ال
+
+174
+00:12:34,910 --> 00:12:38,790
+insoluble complex اسمه crystal violet iodine
+
+175
+00:12:38,790 --> 00:12:43,210
+complex هذا ال complex بينهجز داخل الcell wall
+
+176
+00:12:49,850 --> 00:12:53,850
+تالت مكون من المكونات لـ Gram Stain Kit الـ
+
+177
+00:12:53,850 --> 00:12:58,770
+Ethanol أو الـ Acetone يعتبر Decalarizer مزيل
+
+178
+00:12:58,770 --> 00:13:03,170
+لللون Crystal Violet Iodine Complex فاندي Complex
+
+179
+00:13:03,170 --> 00:13:06,950
+اسمه Crystal Violet Iodine Complex اللي هو الـ
+
+180
+00:13:06,950 --> 00:13:11,490
+Insoluble Complex washed out of a Gram Negative
+
+181
+00:13:11,490 --> 00:13:15,110
+Organism بقوللي هذا ال Complex هيروح من لجرام
+
+182
+00:13:15,110 --> 00:13:18,090
+Negative Bakteria ليش هيروح من لجرام Negative
+
+183
+00:13:18,090 --> 00:13:22,920
+Bakteriaبسبب أنه لا يمكن أن يكون مخطط من خلال
+
+184
+00:13:22,920 --> 00:13:26,200
+مجموعة ليبوغوليسا كارايد لايعني مش هيقدر ينهجز
+
+185
+00:13:26,200 --> 00:13:28,980
+لأنه أنا عندي في الإجرام نيجاتيف بكتيريا
+
+186
+00:13:28,980 --> 00:13:33,440
+ليبوغوليسا كارايد فيه عندي دهون والكحول هيتوب في
+
+187
+00:13:33,440 --> 00:13:39,680
+هذه الدهون ويزيل إلهم صفرانين الصفرانين بسميها ال
+
+188
+00:13:39,680 --> 00:13:43,380
+counter stand أو ال secondary stand simple
+
+189
+00:13:43,380 --> 00:13:48,100
+positive stand اتفقنا أنه هيSimple Stain لأنها
+
+190
+00:13:48,100 --> 00:13:52,080
+بازكستان بتحمل شحنة موجة بقى هتصبغ البكتيريا
+
+191
+00:13:52,080 --> 00:13:57,580
+السالبة ذات Provide Contrasting Dye قولي هي صبغة
+
+192
+00:13:57,580 --> 00:14:01,740
+مايوزية ليش سميتها هكذا لأن ال crystal violet
+
+193
+00:14:01,740 --> 00:14:05,500
+هتعطي اللون الviolet للكل سواء Gram Positive ولا
+
+194
+00:14:05,500 --> 00:14:10,720
+Gram Negative لكن الصفرانين هتميز بين ال Gram
+
+195
+00:14:10,720 --> 00:14:14,940
+Positive و Gram Negativeالـ Gram Positive هتضلها
+
+196
+00:14:14,940 --> 00:14:17,980
+محتفظة باللون الـ Violet لكن الـ Gram Negative
+
+197
+00:14:17,980 --> 00:14:23,180
+هيروح منها اللون هترجح شفافة ففضلي أحط لون يميزها
+
+198
+00:14:23,180 --> 00:14:28,440
+الصفرانين ميزها وأعطاها هذا اللون for decolorized
+
+199
+00:14:28,440 --> 00:14:31,700
+cell هتعطي اللون للبكتيريا اللي راح اللون منها
+
+200
+00:14:31,700 --> 00:14:37,220
+يعني للـ Gram Negative بكتيريا Stains or سلبه اللي
+
+201
+00:14:37,220 --> 00:14:41,620
+بتصبح كل الخلايا but only the negative ones
+
+202
+00:14:41,840 --> 00:14:46,360
+actually a beer pink لكن فقط الـ gram negative
+
+203
+00:14:46,360 --> 00:14:52,580
+بكتيريا هي اللي هتاخد هاي الصبغة و تنسبغ باللون ال
+
+204
+00:14:52,580 --> 00:14:56,320
+pink ليش هتسبغ كل الخلايا لأن بالاصل هي basic
+
+205
+00:14:56,320 --> 00:15:00,280
+stain لكن ال gram positive بكتيريا ما أخدت اللون
+
+206
+00:15:00,280 --> 00:15:06,030
+لأن هي بالاصل ملونة بالنسبة للجدوللو انا صبغت الـ
+
+207
+00:15:06,030 --> 00:15:08,570
+Gram Positive و Gram Negative بيه الـ Primary
+
+208
+00:15:08,570 --> 00:15:11,950
+Stain لـ Crystal Violet سواء كانت Positive او
+
+209
+00:15:11,950 --> 00:15:15,150
+Negative هتاخد اللون الـ purple الـ Mordant
+
+210
+00:15:15,150 --> 00:15:19,490
+الأيودين هيثبت هذا اللون لما اضيف الـ Decolorizing
+
+211
+00:15:19,490 --> 00:15:23,510
+Agent الكحول او الاسيتون مزيل اللون ال Gram
+
+212
+00:15:23,510 --> 00:15:27,450
+Positive بكتيريا هتظل محتفظة باللون لكن ال Gram
+
+213
+00:15:27,450 --> 00:15:31,690
+Negative بكتيريا هيروح اللون منها ويعرفنا ليش
+
+214
+00:15:31,690 --> 00:15:36,140
+السببضفت الـ counter stain الصفرانين لجرام
+
+215
+00:15:36,140 --> 00:15:39,680
+positive بكتيريا هتظل محتفظة باللون لكن لجرام
+
+216
+00:15:39,680 --> 00:15:45,680
+negative هتاخد هذه الصبغة لأنه صارت شفافة فهتاخد
+
+217
+00:15:45,680 --> 00:15:53,560
+هذا اللون تعرفنا
+
+218
+00:15:53,560 --> 00:15:58,000
+على الهدف من لجرام stain و ال principle لجرام
+
+219
+00:15:58,000 --> 00:16:03,100
+stain هلأ بنتعرف على خطوات عمل لجرام stain قلناإنه
+
+220
+00:16:03,100 --> 00:16:06,560
+أي استخدام في الـ Microbiology Lab أول تلات خطوات
+
+221
+00:16:06,560 --> 00:16:10,760
+تابتة أول خطوة الـ Smear preparation وهي تختلف على
+
+222
+00:16:10,760 --> 00:16:14,960
+حسب إذا كان من مجموعة أو من البلاد تاني خطوة الـ
+
+223
+00:16:14,960 --> 00:16:19,060
+Air-drying to prevent lysis تالت خطوة الـ Heat
+
+224
+00:16:19,060 --> 00:16:23,280
+-fixation to let bacteria stick into slide and to
+
+225
+00:16:23,280 --> 00:16:27,800
+kill bacteria الرسمة الموجودة في هذه الصفحة
+
+226
+00:16:27,800 --> 00:16:32,610
+شرحناها نفس ما شرحناها في الصفحة السابقةأول أشياء
+
+227
+00:16:32,610 --> 00:16:36,710
+أن الخلايا كانت transparent هي البكتيريا قلنا أنه
+
+228
+00:16:36,710 --> 00:16:40,810
+من خصائصها transparent شفافة صبغتها بـ crystal
+
+229
+00:16:40,810 --> 00:16:43,830
+violet أخدت اللون سواء إجرام positive أو إجرام
+
+230
+00:16:43,830 --> 00:16:47,650
+negative ضفت الـ decolorizer اللي هو الكفول أو
+
+231
+00:16:47,650 --> 00:16:51,010
+الاسيتون اللون راح من الإجرام negative لكن الإجرام
+
+232
+00:16:51,010 --> 00:16:55,690
+positive دلت محتفظة باللون الviolet ضفت الصفرانين
+
+233
+00:16:55,690 --> 00:16:59,810
+أخدت اللون البكتيريا اللي راح اللون منها اللي هي
+
+234
+00:16:59,810 --> 00:17:01,530
+الإجرام negative بكتيريا
+
+235
+00:17:07,060 --> 00:17:10,680
+الخطوة الرابعه هي الاستان وقلنا انها تختلف حسب نوع
+
+236
+00:17:10,680 --> 00:17:14,100
+الاستان سيمبل استان اجرام استان اصد فاست استان
+
+237
+00:17:14,100 --> 00:17:18,300
+بالنسبالى لجرام استان اول اشيه application of a
+
+238
+00:17:18,300 --> 00:17:21,700
+crystal violet بدنا نضيف ال crystal violet ل
+
+239
+00:17:21,700 --> 00:17:27,460
+primary استان لمدة one minute بعد ما تخلص الدقيقة
+
+240
+00:17:27,460 --> 00:17:32,260
+هغسل بالتعب water تاني اشيه هضيف ال mordant المثبت
+
+241
+00:17:32,260 --> 00:17:36,900
+الايودين برضه لمدة دقيقةبعد ما اتخلص اللي دي هغسل
+
+242
+00:17:36,900 --> 00:17:41,820
+بال water بعدها هضيف الكحول او ال acetone اللي هو
+
+243
+00:17:41,820 --> 00:17:46,360
+ال decolorizer مزيل اللون لكن هانقل وقت 15 second
+
+244
+00:17:46,360 --> 00:17:50,880
+بعد ما اتخلص ال 15 second هغسل بال water اخر خطوة
+
+245
+00:17:50,880 --> 00:17:54,340
+هضيف ال counterstain او ال secondary stain اللي هي
+
+246
+00:17:54,340 --> 00:17:59,100
+السفرانية لمدة one minute بعد ما اخلص هغسل بال
+
+247
+00:17:59,100 --> 00:18:03,520
+water هتكها Jeff Air Drying بعدين اشوفها تحت ال
+
+248
+00:18:03,520 --> 00:18:13,310
+microscope على عدسة40X و100XI بالنسبة
+
+249
+00:18:13,310 --> 00:18:17,750
+للنتائج كيف بدي أكتب نتيجة لـ Gram Stain نتيجة لـ
+
+250
+00:18:17,750 --> 00:18:21,670
+Gram Stain بتتكون من شقين الشق الأول بتعلق هل هى
+
+251
+00:18:21,670 --> 00:18:25,950
+Gram Positive أو Gram Negative لو كان اللون Violet
+
+252
+00:18:25,950 --> 00:18:29,550
+بقى هى Gram Positive لكن لو كان اللون Pink أو Red
+
+253
+00:18:29,550 --> 00:18:33,550
+بقى هى Gram Negativeالشق التاني بيتعلق بال shape
+
+254
+00:18:33,550 --> 00:18:39,070
+هل هي بصلاي عصاوية أو كوكاي كراوية يبقى لو أجيناها
+
+255
+00:18:39,070 --> 00:18:42,230
+في هذه الصورة بدي أكتب نتيجة لـ Gram Stain هشوف
+
+256
+00:18:42,230 --> 00:18:45,910
+اللون اللون Red يبقى هي Gram Negative Bacteria
+
+257
+00:18:45,910 --> 00:18:50,210
+هطلع على شكل البكتيريا هي عصاوية يبقى بصلاي يبقى
+
+258
+00:18:50,210 --> 00:18:54,290
+النتيجة Gram Negative Bacilli بدون ما أكتب ال
+
+259
+00:18:54,290 --> 00:18:58,540
+arrangementالصورة التانية اللون violet يبقى هي
+
+260
+00:18:58,540 --> 00:19:02,480
+إجرام positive بكتيريا الشكل كروي يبقى كوكالي
+
+261
+00:19:02,480 --> 00:19:11,260
+النتيجة كاملة إجرام positive كوكالي error
+
+262
+00:19:11,260 --> 00:19:15,720
+during staining never ever used oil culture بقول
+
+263
+00:19:15,720 --> 00:19:20,280
+ممنوع أستخدم مستعمرات من مزارة قديمةلأس لأن
+
+264
+00:19:20,280 --> 00:19:24,080
+البكتيريا مع طول المدة الجدار الخلوي تبعها بيبدأ
+
+265
+00:19:24,080 --> 00:19:28,560
+يتحلل بالتالي هي بتكون Multilayer اتحلل الجزء منها
+
+266
+00:19:28,560 --> 00:19:32,340
+صارت مبينة كإنها One Layer يعني بيعطيني False
+
+267
+00:19:32,340 --> 00:19:35,380
+Result يعني ممكن البكتيريا تكون بالاصل Gram
+
+268
+00:19:35,380 --> 00:19:38,900
+Positive بكتيريا روحت عملتي Gram Stain أخدت
+
+269
+00:19:38,900 --> 00:19:44,660
+البكتيريا من Old Culture هتبين في نتيجة Gram Stain
+
+270
+00:19:44,660 --> 00:19:49,290
+كإنها Gram Negative هتبين لونها لون Pinkفبالتالي
+
+271
+00:19:49,290 --> 00:19:55,550
+ممنوع أنا أستخدم بكتيريا فيه لـ Gram-S تكون من All
+
+272
+00:19:55,550 --> 00:19:59,750
+culture never ever used sample for patient take
+
+273
+00:19:59,750 --> 00:20:03,130
+antibiotic اللي ما تاخدي بكتيريا معزولة من مريض
+
+274
+00:20:03,130 --> 00:20:06,590
+بياخد antibiotic أحنا قلنا في أول المحاضرة أن
+
+275
+00:20:06,590 --> 00:20:10,390
+المضادات الحيوية ممكن تستهدف ال cell wall بالتحديد
+
+276
+00:20:10,390 --> 00:20:13,830
+بتستهدف ال cross link زي ال penicillin اللي
+
+277
+00:20:13,830 --> 00:20:18,590
+بيستهدف ل gram positive بكتيريا يعنيلو مريض بياخد
+
+278
+00:20:18,590 --> 00:20:23,750
+penicillin عشان يعالج التهاب بكتيري من البكتيريا
+
+279
+00:20:23,750 --> 00:20:29,790
+لجرام positive bacteria هتبين كإنها في جرام
+
+280
+00:20:29,790 --> 00:20:34,010
+negative bacteria في الجرام استخدامي، ليش؟ لأن الـ
+
+281
+00:20:34,010 --> 00:20:36,810
+penicillin بيستهدف الـ cross-link هي كانت
+
+282
+00:20:36,810 --> 00:20:40,830
+multilayer of peptidoglycan هتبين كإنها one layer
+
+283
+00:20:40,830 --> 00:20:45,790
+هيفكك الروابط، هيعمل collapse لـ cell wallTime of
+
+284
+00:20:45,790 --> 00:20:50,510
+Decalarizer بقولنا وقت الـ Decalarizer مهم جدا
+
+285
+00:20:50,510 --> 00:20:55,890
+يعتبر critical لو زودت انا over ال gram positive
+
+286
+00:20:55,890 --> 00:21:00,330
+بكتيريا هتبين كأنها gram negative بكتيريا ليش؟
+
+287
+00:21:00,330 --> 00:21:03,790
+لأنه هيكون في عندي وقت كافي لدخول ال Decalarizer
+
+288
+00:21:03,790 --> 00:21:07,910
+اللي هو الكحول لأ ال multilayer فبالتالي اللون
+
+289
+00:21:07,910 --> 00:21:12,390
+هيروح من ال gram positive بكتيرياهتصبح البكتيريا
+
+290
+00:21:12,390 --> 00:21:16,330
+شفّا فهسبغ بالصفرانين هتاخد اللون ال pink وهتبين
+
+291
+00:21:16,330 --> 00:21:21,030
+كإنها gram negative بكتيريا لو أنا قللت وقت ال
+
+292
+00:21:21,030 --> 00:21:25,350
+decolorizer أقل من 15 second لgram negative
+
+293
+00:21:25,350 --> 00:21:29,550
+بكتيريا هتبين كإنها gram positive بكتيريا ليش؟
+
+294
+00:21:29,550 --> 00:21:33,690
+لأنه مش هيكون في وقت كافي عشان ينزل ال crystal
+
+295
+00:21:33,690 --> 00:21:37,470
+violet من لgram negative بكتيريا من الpeptido
+
+296
+00:21:37,470 --> 00:21:40,930
+glycanهتبقى الـ Gram Negative محتفظة باللون
+
+297
+00:21:40,930 --> 00:21:44,990
+الفيوليت روحت أنا حطيت الصفرانين مش هتاخد الصبغة
+
+298
+00:21:44,990 --> 00:21:49,630
+لأنها ملونة باللون الفيوليت فبالتالي الـ Gram
+
+299
+00:21:49,630 --> 00:21:53,690
+Negative هتبين كإنها Gram Positive time of
+
+300
+00:21:53,690 --> 00:21:57,850
+fixation لو أنا زودت وقت ال fixation أو ضلت حتى ال
+
+301
+00:21:57,850 --> 00:22:01,190
+slide على اللهب ال Gram Positive بكتيريا هتبين
+
+302
+00:22:01,190 --> 00:22:05,350
+كإنها Gram Negative بكتيرياأحنا قلنا الفكساشن
+
+303
+00:22:05,350 --> 00:22:09,930
+بيعمل partial melting of cell wall لو أنا زودت وقت
+
+304
+00:22:09,930 --> 00:22:13,430
+الفكساشن هيصير في عندي complete melting للcell
+
+305
+00:22:13,430 --> 00:22:19,270
+wall يبقى هتبين ال multilayer كأنها one layer
+
+306
+00:22:19,270 --> 00:22:24,150
+هيصير collapse لل cell wall law no sample on slide
+
+307
+00:22:24,150 --> 00:22:28,030
+أحنا قلنا وظيفة الفكساشن عشان تنتصق البكتيريا على
+
+308
+00:22:28,030 --> 00:22:32,110
+ال slide فبالتالي لو كان قليل مش هتنتصق علىالـ
+
+309
+00:22:32,110 --> 00:22:36,310
+slide يبقى مش هيكون فيه عندي عينة هذه أهم الأخطاء
+
+310
+00:22:36,310 --> 00:22:40,750
+اللي ممكن تحصل خلال عملية Gram Staining عشان هيك
+
+311
+00:22:40,750 --> 00:22:45,330
+لازم نلتزم بالوقت في كل خطوة هيك بنكون خلصنا أول
+
+312
+00:22:45,330 --> 00:22:49,210
+نوع من أنواع الـDifferential Stain اللي هو Gram
+
+313
+00:22:49,210 --> 00:22:49,810
+Stain
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/PYxwZLDpRfI.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/PYxwZLDpRfI.srt
new file mode 100644
index 0000000000000000000000000000000000000000..bef8b3b6700ab652764fcb5ed17420b4a171b527
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/PYxwZLDpRfI.srt
@@ -0,0 +1,1518 @@
+
+1
+00:00:03,040 --> 00:00:06,920
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:06,920 --> 00:00:11,440
+جميعا، نستكمل معكم محاضرات مساق الـ Microbiology
+
+3
+00:00:11,440 --> 00:00:16,620
+العملي. وفي آخر محاضرة في هذا المساق، سنتكلم في هذه
+
+4
+00:00:16,620 --> 00:00:20,240
+المحاضرة عن موضوعين. الموضوع الأول: بكتيريا
+
+5
+00:00:20,240 --> 00:00:24,540
+generation time. generation time هو الوقت اللازم
+
+6
+00:00:24,540 --> 00:00:29,410
+للـبكتيريا حتى تنقسم وتضاعف. بالنسبة للموضوع الثاني،
+
+7
+00:00:29,410 --> 00:00:34,750
+Microbial Control Agents، العوامل التي تتحكم في نمو
+
+8
+00:00:34,750 --> 00:00:48,110
+الميكروبات، إما بالتثبيت أو بالقتل. Growth
+
+9
+00:00:48,110 --> 00:00:53,430
+النمو، إيش هو النمو؟ is an orderly increase in the
+
+10
+00:00:53,430 --> 00:00:58,610
+quantity of cellular constituents. النمو هو زيادة في
+
+11
+00:00:58,610 --> 00:01:04,550
+محتويات الخلية البكتيرية، زيادة في الـ Biomass، في
+
+12
+00:01:04,550 --> 00:01:09,350
+الكتلة الحيوية للخلية. النمو على ماذا يعتمد؟ It
+
+13
+00:01:09,350 --> 00:01:12,890
+depends upon the ability of the cell to form new
+
+14
+00:01:12,890 --> 00:01:16,330
+protoplasm from nutrients available in the
+
+15
+00:01:16,330 --> 00:01:21,050
+environment.  بيقول يعتمد على قدرة الخلية أنها
+
+16
+00:01:21,050 --> 00:01:26,850
+تكون protoplasm جديدًا. protoplasm يعني cytoplasm من
+
+17
+00:01:26,850 --> 00:01:31,010
+المواد الغذائية الموجودة في البيئة المحيطة بالخلية.
+
+18
+00:01:31,010 --> 00:01:36,010
+البكتيريا. بقول لي المواد البكتيريا. بعض البكتيريا الـ
+
+19
+00:01:36,010 --> 00:01:40,690
+growth، أنه هو بيعتمد على increase أو يضم increase
+
+20
+00:01:40,690 --> 00:01:44,830
+in cell mass. قلنا الـ growth هو زيادة في الكتلة
+
+21
+00:01:44,830 --> 00:01:49,030
+الحيوية،  ليس فقط زيادة في الكتلة الحيوية، بقول لي كمان
+
+22
+00:01:49,030 --> 00:01:54,590
+and number of ribosomes، زيادة في عدد الـ ribosomes،
+
+23
+00:01:54,590 --> 00:01:58,130
+عدد الـ ribosomes. duplication of the bacterial
+
+24
+00:01:58,130 --> 00:02:02,110
+chromosome. أكيد سيصير عندي تضاعف للمادة
+
+25
+00:02:02,110 --> 00:02:07,490
+الوراثية للبكتيريا، لأنه بنهاية هيتكون عندي خليتين
+
+26
+00:02:07,490 --> 00:02:11,610
+بكتيريتين. كل بكتيرية ستأخذ chromosome. synthesis
+
+27
+00:02:11,610 --> 00:02:15,770
+of a new cell wall and plasma membrane.  برضه هيتكون
+
+28
+00:02:15,770 --> 00:02:19,150
+cell wall جديد و plasma membrane جديد، لأنه احنا
+
+29
+00:02:19,150 --> 00:02:25,600
+قلنا سيتكون عندي to cell. فبالتالي كل خلية بكتيرية
+
+30
+00:02:25,600 --> 00:02:31,100
+سيكون لها cell wall منفصل، plasma membrane منفصل،
+
+31
+00:02:31,100 --> 00:02:37,320
+مكونات chromosome منفصلة أيضًا. بقول لي partitioning of
+
+32
+00:02:37,320 --> 00:02:43,100
+the two chromosomes. سيصير عندي انقسام لـ
+
+33
+00:02:43,100 --> 00:02:46,760
+chromosomes، لـ two chromosomes، لأن كل خلية بكتيرية
+
+34
+00:02:46,760 --> 00:02:51,290
+ستأخذ chromosome. Septum Formation، ما معنى Septum
+
+35
+00:02:51,290 --> 00:02:55,950
+Formation؟ يعني اللي هو سيتكون عندي حاجز، قبل
+
+36
+00:02:55,950 --> 00:02:59,110
+ما يصير عندي cell division، سيصير عندي تكوين
+
+37
+00:02:59,110 --> 00:03:04,270
+حاجز. تمام. بعدها سيصير عندي الانقسام. سنشوف في الـ
+
+38
+00:03:04,270 --> 00:03:08,890
+slide، الذي يوضح هذا الحاجز. بالنهاية، ستنقسم
+
+39
+00:03:08,890 --> 00:03:12,530
+الخلية البكتيرية إلى خليتين بكتيريتين. سيصير عندي
+
+40
+00:03:12,530 --> 00:03:17,040
+cell division،  اللي هو الانقسام بواسطة الـ Binary
+
+41
+00:03:17,040 --> 00:03:21,360
+Fission، اللي هو الانشطار الثنائي. شو هو الانشطار
+
+42
+00:03:21,360 --> 00:03:24,940
+الثنائي؟ بقول لي this a sexual process of
+
+43
+00:03:24,940 --> 00:03:28,360
+reproduction is called binary fission. اللي هي
+
+44
+00:03:28,360 --> 00:03:34,480
+عملية لاجنسية،  ليست جنسية، a sexual process،
+
+45
+00:03:34,480 --> 00:03:39,400
+لأن بالنهاية أنا بتكون، أو الانقسام يصير من خلية
+
+46
+00:03:39,400 --> 00:03:44,890
+واحدة، من mother cell، ليست من خليتين، ليست من أب وأم.
+
+47
+00:03:44,890 --> 00:03:49,950
+فبالتالي هي لاجنسية، عملية لاجنسية، asexual process،
+
+48
+00:03:49,950 --> 00:03:53,470
+اللي هي الـ binary fission، اللي هي الانشطار
+
+49
+00:03:53,470 --> 00:03:58,230
+الثنائي. كيف
+
+50
+00:03:58,230 --> 00:04:02,790
+يصير الـ binary division أو الـ cell division لخلية
+
+51
+00:04:02,790 --> 00:04:06,250
+البكتيريا؟ نلاحظ في الصورة أن لدي خلية
+
+52
+00:04:06,250 --> 00:04:12,410
+بكتيرية فيها الـ DNA، المادة الوراثية. الـ DNA سيصير
+
+53
+00:04:12,410 --> 00:04:16,310
+له اللي هو تضاعف، عندي سيصير DNA replication. نلاحظ
+
+54
+00:04:16,310 --> 00:04:20,490
+في الصورة أن والله تضاعف اللي هو الـ Circular DNA.
+
+55
+00:04:20,490 --> 00:04:25,550
+طبعًا خلية البكتيريا، قلنا في أول معمل هو عبارة عن
+
+56
+00:04:25,550 --> 00:04:30,670
+Circular، دائري، ليس linear، مثل الإنسان. سيصير عندي
+
+57
+00:04:30,670 --> 00:04:35,220
+DNA replication، تضاعف للـ DNA. سيصير فيه cell
+
+58
+00:04:35,220 --> 00:04:39,840
+elongation، سيصير استطالة لخلية البكتيريا. بعد كده
+
+59
+00:04:39,840 --> 00:04:44,300
+البكتيريا ستحضر نفسها للانقسام. سيصير عندي
+
+60
+00:04:44,300 --> 00:04:48,860
+يقول لي تكوين حاجز، septum formation، اللي هو يتكون
+
+61
+00:04:48,860 --> 00:04:54,780
+عندي حاجز، حتى إن الخلية البكتيرية تحضر نفسها
+
+62
+00:04:54,780 --> 00:05:00,580
+للانقسام إلى خليتين بشكل كامل، وتبتعد عن بعض. نلاحظ في
+
+63
+00:05:00,580 --> 00:05:06,030
+الصورة هنا أنه صار عندي اللي هو انفصال لخليتين
+
+64
+00:05:06,030 --> 00:05:09,890
+البكتيريتين عن بعض، اللي هو الـ two daughter cells
+
+65
+00:05:09,890 --> 00:05:14,170
+separated. بعدها صار عندي اللي هو كل خلية
+
+66
+00:05:14,170 --> 00:05:19,130
+بكتيرية انفصلت، صار لها DNA خاص بها، و كما هو ملاحظ
+
+67
+00:05:19,130 --> 00:05:24,150
+عبارة عن اللي هو Circular، دائري طبعًا، لها cell
+
+68
+00:05:24,150 --> 00:05:27,930
+wall خاص بها، cytoplasm خاص بها، cell membrane خاص
+
+69
+00:05:27,930 --> 00:05:31,070
+بها، chromosome خاص بها، كما اتكلمنا في الـ slide
+
+70
+00:05:31,070 --> 00:05:39,940
+الذي قبله. هذا بالنسبة لـ Cell Division. في
+
+71
+00:05:39,940 --> 00:05:44,340
+هذه الـ slide، سنتعرف على بعض المصطلحات المهمة. أول
+
+72
+00:05:44,340 --> 00:05:47,820
+مصطلح، اللي هو الـ Growth. بالنسبة لـ Growth، عرفناه
+
+73
+00:05:47,820 --> 00:05:51,940
+في الـ slide السابق، وقلنا اللي هو زيادة في الكتلة
+
+74
+00:05:51,940 --> 00:05:56,460
+الحيوية. The Equation of Biomass، اللي هو زيادة في
+
+75
+00:05:56,460 --> 00:06:00,240
+الكتلة الحيوية، leading to cell division. بالنهاية،
+
+76
+00:06:00,240 --> 00:06:04,920
+سيصير عندي انقسام للخلية إلى خليتين بكتيريتين. or
+
+77
+00:06:04,920 --> 00:06:09,920
+reproduction.  ثاني مصطلح: a batch culture. الـ batch
+
+78
+00:06:09,920 --> 00:06:14,200
+culture هو نظام مغلق، in a broth
+
+79
+00:06:14,200 --> 00:06:18,020
+medium in which no additional nutrient. واللي هو
+
+80
+00:06:18,020 --> 00:06:22,040
+نظام مغلق في الـ broth media. لا يصير عندي أي
+
+81
+00:06:22,040 --> 00:06:26,550
+إضافات للمواد الغذائية.  Bad is added after
+
+82
+00:06:26,550 --> 00:06:30,230
+incubation of the broth. بعد ما أنا أزرع هذه
+
+83
+00:06:30,230 --> 00:06:34,610
+البكتيريا. يعني بكون عندي ميديا، وأزرع فيها
+
+84
+00:06:34,610 --> 00:06:40,610
+البكتيريا، وأحطها في الـ incubator. لو خلصت المغذيات،
+
+85
+00:06:40,610 --> 00:06:44,050
+و البكتيريا في الـ incubator، أو بعد ما أنا أخرج هذا
+
+86
+00:06:44,050 --> 00:06:48,630
+الطبق من الـ incubator، تموت البكتيريا. لكن هذا
+
+87
+00:06:48,630 --> 00:06:52,800
+بالنسبة لـ batch culture، في النظام المغلق. في النظام
+
+88
+00:06:52,800 --> 00:06:57,320
+المغلق، أنا لا أضيف أي مغذيات بعد اللي هو الزراعة.
+
+89
+00:06:57,320 --> 00:07:02,460
+خلاص، إذا خلصت المغذيات البكتيريا تموت. لكن بالنسبة
+
+90
+00:07:02,460 --> 00:07:06,340
+لـ continuous culture، عندي أنا نظام مفتوح. هذا
+
+91
+00:07:06,340 --> 00:07:10,580
+نظام، كل ما تنفد المواد الغذائية، أنا أضيف غيرها.
+
+92
+00:07:10,580 --> 00:07:15,960
+فبالتالي، في نظام الـ continuous culture، لا تمر
+
+93
+00:07:15,960 --> 00:07:20,510
+البكتيريا بمرحلة اللي هو الـ death phase، في مرحلة
+
+94
+00:07:20,510 --> 00:07:24,150
+الموت، لأني أنا أضيف مواد غذائية. لكن في الـ
+
+95
+00:07:24,150 --> 00:07:27,830
+closed system، اللي هو الـ Batch Culture، لا أضيف أي
+
+96
+00:07:27,830 --> 00:07:32,350
+مغذيات. No additional nutrient بعد الزراعة. فبالتالي
+
+97
+00:07:32,350 --> 00:07:36,290
+البكتيريا تموت، وتمر بمرحلة اللي هو الـ Death
+
+98
+00:07:36,290 --> 00:07:41,250
+phase. بالنسبة لـ Generation Time، اتكلمنا عنه. Time
+
+99
+00:07:41,250 --> 00:07:44,510
+taken for a cell population to double in number. هو
+
+100
+00:07:44,510 --> 00:07:49,200
+الوقت الذي يلزم للخلية لتضاعف في العدد. هو
+
+101
+00:07:49,200 --> 00:07:52,600
+بقول يساوي the average length of the cell
+
+102
+00:07:52,600 --> 00:07:59,120
+cycle. هذا بالنسبة لهذه المصطلحات التي ستمر معنا
+
+103
+00:07:59,120 --> 00:08:05,340
+خلال الـ chapter. الـ
+
+104
+00:08:05,340 --> 00:08:09,680
+bacterial growth curve، اللي هو منحنى نمو البكتيريا.
+
+105
+00:08:09,680 --> 00:08:14,700
+عندي أربع أطوار أو أربع stages تمر بها الخلية
+
+106
+00:08:14,700 --> 00:08:18,910
+البكتيرية في النمو. four characteristic phases of the
+
+107
+00:08:18,910 --> 00:08:22,910
+growth cycle. اللي هم الـ lag phase، الـ log phase، الـ
+
+108
+00:08:22,910 --> 00:08:27,050
+stationary phase، الـ death phase. سنتكلم عن كل phase
+
+109
+00:08:27,050 --> 00:08:31,070
+بالتفصيل. أول phase، اللي هو الـ lag phase، اللي هو
+
+110
+00:08:31,070 --> 00:08:35,650
+التطور التحضيري للانقسام. هذا التطور، نعطي فرصة
+
+111
+00:08:35,650 --> 00:08:38,970
+للـبكتيريا لتتأقلم، ليصير عندي adaptation،
+
+112
+00:08:38,970 --> 00:08:44,130
+تحضر نفسها للانقسام، تحضر الإنزيمات للانقسام. لكن
+
+113
+00:08:44,130 --> 00:08:47,370
+فعليًا، لا يصير عندي أي نمو، لا يصير عندي أي
+
+114
+00:08:47,370 --> 00:08:52,630
+انقسام، لا يصير عندي زيادة في العدد. متى هذا الـ
+
+115
+00:08:52,630 --> 00:08:56,350
+phase يصير؟ immediately after the incubation of the
+
+116
+00:08:56,350 --> 00:09:00,190
+cells into fresh media. بقول لي وقت ما أنتي تزرعي
+
+117
+00:09:00,190 --> 00:09:04,390
+الخلايا في الـ fresh media، ستبدأ مرحلة الـ lag
+
+118
+00:09:04,390 --> 00:09:08,350
+phase. الـ population، بقول لي الـ population remained
+
+119
+00:09:08,350 --> 00:09:13,670
+temporarily unchanged. بقول لي لا يصير عندي أي
+
+120
+00:09:13,670 --> 00:09:17,790
+تغيير في العدد. تمام، لا يصير أي تغيير في العدد
+
+121
+00:09:17,790 --> 00:09:22,030
+بشكل مؤقت. although there is no apparent cell
+
+122
+00:09:22,030 --> 00:09:25,050
+division. بقول لي لا يصير عندي أي انقسام. اتفاقنا
+
+123
+00:09:25,050 --> 00:09:28,570
+لا يصير عندي أي نمو. the cells may be growing in
+
+124
+00:09:28,570 --> 00:09:33,230
+volume. لكن ممكن الخلايا يصير عندي زيادة في
+
+125
+00:09:33,230 --> 00:09:37,430
+الحجم، الـ volume، وليس العدد. لا يصير عندي زيادة
+
+126
+00:09:37,430 --> 00:09:42,000
+في العدد. قلنا الـ population يظل طبعًا بشكل مؤقت
+
+127
+00:09:42,000 --> 00:09:46,040
+ثابتًا، لا يتغير. لكن بقول لي ممكن يصير عندي زيادة في
+
+128
+00:09:46,040 --> 00:09:50,280
+الحجم، في الـ biomass، لكن ليس في العدد. يصير عندي
+
+129
+00:09:50,280 --> 00:09:54,240
+synthesizing للإنزيمات، أكيد تحضر نفسها للانقسام.
+
+130
+00:09:54,240 --> 0:10:00,300
+فبالتالي يتم إنتاج إنزيمات، بروتين، RNA، increasing
+
+131
+00:10:00,300 --> 00:10:04,700
+in metabolic activity. كل هذه الأمور تصير عشان
+
+132
+00:10:04,700 --> 00:10:09,180
+تحضر نفسها للانقسام، لكن لا يصير عندي أي نمو.
+
+133
+00:10:17,300 --> 00:10:21,120
+هذه الرسمة تعبر عن مراحل نمو البكتيريا. قلنا فيه
+
+134
+00:10:21,120 --> 00:10:26,000
+عندي four stages: lag، log، stationary، death phases.
+
+135
+00:10:26,000 --> 00:10:31,000
+أول مرحلة أو أول stage اللي هو الـ lag phase، قلنا
+
+136
+00:10:31,000 --> 00:10:36,380
+أن عدد البكتيريا في هذا الطور يظل ثابتًا، un-
+
+137
+00:10:36,380 --> 00:10:41,740
+changed. طبعًا، عندي في هذا المحور اللي هو
+
+138
+00:10:41,740 --> 00:10:45,640
+الـ time، وهذا المحور عدد البكتيريا. عدد البكتيريا في
+
+139
+00:10:45,640 --> 00:10:50,470
+الـ lag phase يظلوا لا يتغير، يظلوا ثابتين، كما
+
+140
+00:10:50,470 --> 00:10:54,150
+نلاحظ في الصورة أن العدد ثابت في مرحلة الـ lag
+
+141
+00:10:54,150 --> 00:10:58,550
+phase. بقول لي the length of the lag phase is
+
+142
+00:10:58,550 --> 00:11:03,010
+apparently dependent on a wide variety of factors،
+
+143
+00:11:03,010 --> 00:11:08,210
+including. ما هي العوامل التي تؤثر على طول الـ lag
+
+144
+00:11:08,210 --> 00:11:13,810
+phase؟  ممكن مثلًا في بكتيريا يكون الـ lag phase 50 أو
+
+145
+00:11:13,810 --> 00:11:18,850
+كما هو ملاحظ في الصورة، يعني تقريبًا 20 minute. في
+
+146
+00:11:18,850 --> 00:11:22,490
+بكتيريا أخرى، ممكن يكون مثلًا 50 minute أو
+
+147
+00:11:22,490 --> 00:11:27,350
+30 minute. فيختلف طول الـ lag phase من بكتيريا
+
+148
+00:11:27,350 --> 00:11:31,430
+لبكتيريا.  هناك عدة عوامل تؤثر على
+
+149
+00:11:31,430 --> 00:11:35,950
+اللي هو طول الـ lag phase. طول مدة الـ lag phase. الآن،
+
+150
+00:11:35,950 --> 00:11:39,820
+بالنسبة للـ generation time. الـ generation time لكل
+
+151
+00:11:39,820 --> 00:11:45,880
+بكتيريا مختلف، ليس نفس الشيء في كل البكتيريات طبعًا.
+
+152
+00:11:45,880 --> 00:11:50,100
+ذكرنا، عندما شرحنا الـ acid fast stain، اللي هي
+
+153
+00:11:50,100 --> 00:11:53,760
+البكتيريا الـ Mycobacterium tuberculosis، تحتاج
+
+154
+00:11:53,760 --> 00:12:00,200
+20 ساعة لتتنمو. في نوع بكتيريا آخر، خلال مثلًا ربع
+
+155
+00:12:00,200 --> 00:12:04,080
+ساعة، ثلث ساعة، تنمو البكتيريا. فبالتالي، الـ
+
+156
+00:12:04,080 --> 00:12:07,100
+generation time، الوقت اللازم لنمو البكتيريا،
+
+157
+00:12:07,100 --> 00:12:12,040
+يختلف من بكتيريا لأخرى. الـ lag phase نفس الشيء، ليس
+
+158
+00:12:12,040 --> 00:12:15,920
+كل البكتيريا تتكيف بنفس الدرجة. هناك عوامل
+
+159
+00:12:15,920 --> 00:12:21,520
+تؤثر على طول الـ lag phase. أول عامل: the size of
+
+160
+00:12:21,520 --> 00:12:26,900
+the inoculum.  اللي هو كمية البكتيريا التي زرعتها
+
+161
+00:12:26,900 --> 00:12:30,940
+في الـ growth media. حجم الـ inoculum، طبعًا أكيد
+
+162
+00:12:30,940 --> 00:12:34,540
+البكتيريا القليلة ستحضر نفسها بشكل أسرع. لكن
+
+163
+00:12:34,540 --> 00:12:39,160
+البكتيريا، لو أنا أخذت inoculum وكان الحجم أكبر، ستأخذ
+
+164
+00:12:39,160 --> 00:12:43,540
+وقتًا أطول. لكن البكتيريا، لو أنا أخذت inoculum
+
+165
+00:12:43,540 --> 00:12:49,060
+وكان الحجم قليلًا، أكيد ستحضر نفسها بشكل أسرع. يبقى
+
+166
+00:12:49,060 --> 00:12:53,740
+هذا بالنسبة لأول عامل يؤثر على الـ lag phase. ثاني
+
+167
+00:12:53,740 --> 00:12:59,110
+عامل:  الوقت الذي يجب أن يتجاوز من المصادر الواقعية
+
+168
+00:12:59,110 --> 00:13:05,050
+أو من المصادر الواقعية. بقول لي الوقت اللازم حتى أن
+
+169
+00:13:05,050 --> 00:13:08,790
+البكتيريا تتكيف، في حال صار عندها صدمة خلال
+
+170
+00:13:08,790 --> 00:13:13,790
+عملية النقل. البكتيريا، قبل ما أنا أزرعها في اللي هو
+
+171
+00:13:13,790 --> 00:13:16,990
+الـ media، أكيد تكون في وسط، ممكن تكون في تربة، في
+
+172
+00:13:16,990 --> 00:13:21,690
+هواء، في طعام. فبالتالي، خلال الـ transfer، ممكن تكون
+
+173
+00:13:21,690 --> 00:13:26,620
+تعرضت لـ shock، لـ physical damage. الوقت الذي تحتاجه
+
+174
+00:13:26,620 --> 00:13:30,660
+هذه البكتيريا لتتكيف مع الوسط الذي وضعته
+
+175
+00:13:
+
+223
+00:17:15,380 --> 00:17:20,720
+bacterial culture يبقى لو أنا حكيت generation time
+
+224
+00:17:20,720 --> 00:17:25,320
+يبقى أنا بعبر عن اللي هو الـ X potential phase برضه
+
+225
+00:17:25,320 --> 00:17:30,220
+له اسم تاني اللي هو الـ doubling time لأنه قلنا أن
+
+226
+00:17:30,220 --> 00:17:35,300
+البكتيريا بيصير لها تضاعف doubling يعني تضاعف ففي
+
+227
+00:17:35,300 --> 00:17:45,040
+هذه phase البكتيريا بتتضعف بمعدل ثابت بالنسبة
+
+228
+00:17:45,040 --> 00:17:50,520
+للمرحلة اللي هي الـ stationary phase البكتيريا لما
+
+229
+00:17:50,520 --> 00:17:54,220
+تموت بتنتج فضلات بتنتج waste بعدها رح تبدأ
+
+230
+00:17:54,220 --> 00:17:58,600
+البكتيريا تموت لما أوصل لمرحلة أنه البكتيريا أو
+
+231
+00:17:58,600 --> 00:18:02,120
+عدد البكتيريا اللي بتنمو بيساوي عدد البكتيريا اللي
+
+232
+00:18:02,120 --> 00:18:07,580
+بتموت. بسمي هذه المرحلة مرحلة الثبات stationary phase
+
+233
+00:18:07,580 --> 00:18:12,400
+طبعا الـ rate of growth معدل النمو في مرحلة الثبات
+
+234
+00:18:12,400 --> 00:18:17,020
+بيساوي صفر. exponential growth cannot be continue
+
+235
+00:18:17,020 --> 00:18:20,840
+forever in a batch culture for example, closed
+
+236
+00:18:20,840 --> 00:18:25,340
+system such as a closed system أو بقولي انه في ال
+
+237
+00:18:25,340 --> 00:18:30,140
+exponential growth ما بيستمر في الـ batch culture
+
+238
+00:18:30,510 --> 00:18:34,470
+واتفقنا على هذه المعلومة لما شرحنا شو هي الـ Batch
+
+239
+00:18:34,470 --> 00:18:38,350
+Culture. الـ Batch Culture قلنا هي نظام مغلق ما
+
+240
+00:18:38,350 --> 00:18:42,810
+بضيف مواد غذائية بعد الـ inoculation فبالتالي لما
+
+241
+00:18:42,810 --> 00:18:47,490
+توصل البكتيريا لمرحلة أو لما الـ media يصير فيها
+
+242
+00:18:47,490 --> 00:18:51,410
+نقص في المواد الغذائية، تخلص المواد الغذائية أكيد
+
+243
+00:18:51,410 --> 00:18:58,490
+البكتيريا هتبدأ تموت فبالتالي أنا هوصل لمرحلة اللي
+
+244
+00:18:58,490 --> 00:19:02,470
+هو الـ stationary phase ومرحلة الـ death phase. الـ
+
+245
+00:19:02,470 --> 00:19:07,190
+X potential phase إجراث مش هيظل مستمر لكن هذا بالنسبة لل
+
+246
+00:19:07,190 --> 00:19:10,570
+batch culture لكن في الـ continuous culture اللي
+
+247
+00:19:10,570 --> 00:19:14,450
+قلنا أنا بضيف مواد غذائية فبالتالي الـ X potential
+
+248
+00:19:14,450 --> 00:19:19,810
+phase إجراث هيظل مستمر. في الـ continuous culture بيكون
+
+249
+00:19:19,810 --> 00:19:23,330
+ما في عندي مرحلة اللي هو الـ death phase في الـ
+
+250
+00:19:23,330 --> 00:19:26,830
+continuous culture. في الـ open system راح توقف لأن
+
+251
+00:19:26,830 --> 00:19:30,710
+مرحلة الثبات وبعدها راح أضيف مقذيات وترجع تنمو.
+
+252
+00:19:30,710 --> 00:19:35,450
+بقول لي إنه في مرحلة الثبات قد إيش راح تبقى فيها
+
+253
+00:19:35,450 --> 00:19:38,650
+بكتيريا، بيعتمد على عدة عوامل. population growth is
+
+254
+00:19:38,650 --> 00:19:42,230
+limited by one of three factors. أول عامل اللي
+
+255
+00:19:42,230 --> 00:19:46,390
+هو استهلاك المواد الغذائية. هل سيكون عندي نقص في
+
+256
+00:19:46,390 --> 00:19:53,030
+المقذيات؟ ثاني عامل اللي هو زيادة الـ waste، زيادة الـ
+
+257
+00:19:53,030 --> 00:19:56,730
+waste نتيجة العمليات الأيضية، وتراكمه هذا أكيد
+
+258
+00:19:56,730 --> 00:20:00,910
+هيأثر في اللي هو قد إيش راح تظل البكتيريا
+
+259
+00:20:00,910 --> 00:20:07,330
+البكتيريا طبعا حية. ثالث factor اللي هو
+
+260
+00:20:07,330 --> 00:20:12,570
+نقص المساحة، نقص المساحة في الأنبوب نتيجة زيادة عدد
+
+261
+00:20:12,570 --> 00:20:16,390
+الخلايا، يبطل فيها  انديسع اللي هو a lack of
+
+262
+00:20:16,390 --> 00:20:21,010
+biological space، اللي هو نقص المساحة الحيوية، بيوقف
+
+263
+00:20:21,010 --> 00:20:25,810
+النمو. فبالتالي، هذه كل العوامل اللي بتأثر في
+
+264
+00:20:25,810 --> 00:20:30,150
+مرحلة الثبات، قد إيش راح تبقى فيها بكتيريا، بيعتمد على هذه
+
+265
+00:20:30,150 --> 00:20:35,010
+العوامل. بالنسبة لرابع مرحلة اللي هي الـ death phase،
+
+266
+00:20:35,010 --> 00:20:38,450
+if incubation continues after the population
+
+267
+00:20:38,450 --> 00:20:42,380
+reaches stationary phase، بقول لي، لو الـ incubation ظل
+
+268
+00:20:42,380 --> 00:20:45,540
+مستمر بعد ما أوصل لمرحلة الـ stationary phase اللي
+
+269
+00:20:45,540 --> 00:20:49,760
+هو معدل إنه بيكون فيها ثابت، a death phase follows
+
+270
+00:20:49,760 --> 00:20:55,040
+بعدها بتبدأ مرحلة الـ death phase in which the
+
+271
+00:20:55,040 --> 00:20:58,960
+viable cell population decline، بقول لي إنه عدد
+
+272
+00:20:58,960 --> 00:21:02,260
+الخلايا اللي بتنمو في الـ death phase ها بتبدأ
+
+273
+00:21:02,260 --> 00:21:05,760
+تقل. في مرحلة الـ stationary قلنا إن الـ viable
+
+274
+00:21:05,760 --> 00:21:09,820
+cells بتساوي اللي هي الخلايا الحية بتساوي الخلايا
+
+275
+00:21:09,820 --> 00:21:13,820
+الميتة. لكن في الـ death phase الـ viable cells
+
+276
+00:21:13,820 --> 00:21:18,520
+الخلايا الحية بتبدأ تقل. هندقلي ملاحظة أنا قولتها
+
+277
+00:21:18,520 --> 00:21:22,880
+في chapter الـ Serial Dilution. if counting by
+
+278
+00:21:22,880 --> 00:21:25,580
+turbidometric measurement اللي هو الـ
+
+279
+00:21:25,580 --> 00:21:30,560
+spectrophotometer or microscopic count, the death
+
+280
+00:21:30,560 --> 00:21:34,600
+phase cannot be observed. قلنا إنه أنا ما بميز
+
+281
+00:21:34,600 --> 00:21:37,780
+الخلايا الحية والخلايا الميتة لو أنا عديت في الـ
+
+282
+00:21:37,780 --> 00:21:41,600
+turbidometric measurement أو لو استخدمت الـ
+
+283
+00:21:41,600 --> 00:21:44,900
+microscopic count، لو استخدمت الـ microscope ما
+
+284
+00:21:44,900 --> 00:21:49,160
+بفرق بين الخلايا الحية والخلايا الميتة. فقط في
+
+285
+00:21:49,160 --> 00:21:54,440
+الطريقة اللي هي الـ viable cells count اللي هي الـ plate
+
+286
+00:21:54,440 --> 00:21:58,340
+count هي اللي بميز الخلايا الحية والميتة، هي اللي
+
+287
+00:21:58,340 --> 00:22:02,400
+بتعد فقط الـ viable cells، ما بتعد الخلايا الميتة
+
+288
+00:22:02,400 --> 00:22:07,340
+لأن الأكيد البكتيريا اللي ماتت مش هتنمو على الطبق
+
+289
+00:22:08,010 --> 00:22:11,150
+فبالتالي لو أنا استخدمت هدول الطريقتين مش هلاحظ
+
+290
+00:22:11,150 --> 00:22:15,210
+الـ death phase. فقط في اللي هو الـ plate count هذه
+
+291
+00:22:15,210 --> 00:22:18,110
+الملاحظة طبعا، وأنا ذكرتها في الـ serial dilution
+
+292
+00:22:18,110 --> 00:22:22,450
+نكمل. during the death phase the number of viable
+
+293
+00:22:22,450 --> 00:22:27,630
+cells decreases. قلنا إنه في الـ death phase الخلايا
+
+294
+00:22:27,630 --> 00:22:32,750
+الحية بتبدأ تقل بشكل اللي هو logarithmic. تمام نفس
+
+295
+00:22:32,750 --> 00:22:36,170
+اللي هو في الـ X potential phase الـ growth قلنا بيبدأ
+
+296
+00:22:36,170 --> 00:22:40,610
+يزيد عدد الخلايا بشكل لوغاريتمي، بشكل أسي لكن هنا
+
+297
+00:22:40,610 --> 00:22:45,790
+بيبدأ يقل بشكل لوغاريتمي. essentially the reverse of
+
+298
+00:22:45,790 --> 00:22:49,070
+the growth during the log phase. بقول لي بيكون في شكل
+
+299
+00:22:49,070 --> 00:22:53,010
+عكسي للـ log phase لأن الـ log phase بيزيد بشكل
+
+300
+00:22:53,010 --> 00:22:58,410
+لوغاريتمي لكن بالنسبة للـ death phase بيقل بشكل لوغاريتمي
+
+301
+00:22:58,820 --> 00:23:04,900
+هكذا خلصنا مراحل نمو البكتيريا، وشرحنا كل الـ stages
+
+302
+00:23:04,900 --> 00:23:10,160
+اللي بتمر فيها البكتيريا، من مرحلة الـ lag phase،
+
+303
+00:23:10,160 --> 00:23:13,520
+الـ log phase، الـ stationary phase، وآخر شيء
+
+304
+00:23:13,520 --> 00:23:17,140
+اللي هو الـ death phase. طبعا هذا كله في الـ batch
+
+305
+00:23:17,140 --> 00:23:21,660
+culture. هاني
+
+306
+00:23:21,660 --> 00:23:26,190
+موضح مراحل نمو البكتيريا في صورة. قلنا في عندي أربع
+
+307
+00:23:26,190 --> 00:23:30,010
+stages اللي هو الـ lag phase، اللي هو اللي أنا من
+
+308
+00:23:30,010 --> 00:23:34,810
+وقت ما زرعت اللي هو الـ inoculum في الـ media، في الـ
+
+309
+00:23:34,810 --> 00:23:39,310
+fresh media. بعدها هتبدأ الخلايا تنمو، يصير في عندي
+
+310
+00:23:39,310 --> 00:23:43,150
+viable cells اللي هي الخلايا الحية في الـ X
+
+311
+00:23:43,150 --> 00:23:47,710
+potential phase، بتبدأ يزيد عدد الخلايا الحية في
+
+312
+00:23:47,710 --> 00:23:50,950
+الـ stationary phase. قلنا إنه بتبدأ الخلايا هنا
+
+313
+00:23:50,950 --> 00:23:53,730
+تتموت، بنلاحظ أنه هنا في عندي اللون الزهري، بدل على
+
+314
+00:23:54,220 --> 00:23:57,620
+الـ death cells، الخلايا اللي بتتموت. بتبدأ الخلايا
+
+315
+00:23:57,620 --> 00:24:02,300
+أنها تموت، بكون عدد الخلايا اللي بتنمو بيساوي عدد
+
+316
+00:24:02,300 --> 00:24:05,980
+الخلايا اللي بتموت في مرحلة الـ stationary phase.
+
+317
+00:24:05,980 --> 00:24:10,940
+بعدين في مرحلة الـ death phase اللي هي بتبدأ اللي
+
+318
+00:24:10,940 --> 00:24:17,740
+هو عدد الخلايا اللي حية بيبدأ يقل بشكل لوغاريتمي. هذا
+
+319
+00:24:17,740 --> 00:24:20,480
+بالنسبة لمراحل نمو البكتيريا.
+
+320
+00:24:23,210 --> 00:24:26,930
+Growth in continuous culture. A continuous culture
+
+321
+00:24:26,930 --> 00:24:30,470
+is an open system. اتفقنا إن الـ continuous culture
+
+322
+00:24:30,470 --> 00:24:34,510
+هي عبارة عن نظام مفتوح، على عكس الـ batch culture
+
+323
+00:24:34,510 --> 00:24:37,730
+اللي كانت نظام مغلق. in which fresh media is
+
+324
+00:24:37,730 --> 00:24:40,410
+continuously added to the culture at a constant
+
+325
+00:24:40,410 --> 00:24:44,450
+rate and all the broth is removed at the same rate.
+
+326
+00:24:44,450 --> 00:24:48,870
+إيش قصده في هذه الجملة؟ إنه بنفس الوقت اللي أنا بدي
+
+327
+00:24:48,870 --> 00:24:54,450
+أضيف فيه مغذيات، نفس هذا الوقت هسحب الفضلات. يبقى
+
+328
+00:24:54,450 --> 00:24:57,870
+المواد الغذائية اللي أنا بدي أضيفها للـ culture
+
+329
+00:24:57,870 --> 00:25:01,150
+لأن إحنا اتفقنا إن الـ continuous culture بضيف فيها
+
+330
+00:25:01,150 --> 00:25:05,190
+مواد غذائية بعد الـ inoculation، ممكن أضيف مواد
+
+331
+00:25:05,190 --> 00:25:08,430
+غذائية لما تخلص المواد الغذائية، ممكن أن أنا أضيف
+
+332
+00:25:08,430 --> 00:25:12,690
+مواد غذائية بعد الـ inoculation. فبالتالي المواد
+
+333
+00:25:12,690 --> 00:25:17,230
+الغذائية اللي أنا ضفتها، هتكون بنفس الوقت اللي أنا
+
+334
+00:25:17,230 --> 00:25:21,570
+بدي أسحب فيه الفضلات. هيكون الاثنين بنفس الـ rate. كيف
+
+335
+00:25:21,570 --> 00:25:26,490
+بدي أسحب؟ بقول لي هذه العملية بستخدم جهاز اسمه الـ
+
+336
+00:25:26,490 --> 00:25:32,170
+chemostat. هذا مهم، هذا الجهاز اللي أنا بدي أضيف
+
+337
+00:25:32,170 --> 00:25:35,510
+فيه اللي هو، بنفس الوقت اللي بدي أضيف فيه
+
+338
+00:25:35,510 --> 00:25:40,530
+مغذيات، هسحب فضلات، نفس الوقت، بنفس الـ rate، نفس
+
+339
+00:25:40,530 --> 00:25:44,470
+المعدل. التركيز في الـ cells سيصل إلى مستوى
+
+340
+00:25:44,470 --> 00:25:48,290
+إتزان. يبقى مستوى مستوى مستوى مستوى مستوى
+
+341
+00:25:48,290 --> 00:25:49,450
+مستوى مستوى مستوى مستوى مستوى مستوى مستوى مستوى
+
+342
+00:25:49,450 --> 00:25:50,070
+مستوى مستوى مستوى مستوى مستوى مستوى مستوى مستوى
+
+343
+00:25:50,070 --> 00:25:51,390
+مستوى مستوى مستوى مستوى مستوى مستوى مستوى مستوى
+
+344
+00:25:51,390 --> 00:25:52,150
+مستوى مستوى مستوى مستوى مستوى مستوى مستوى مستوى
+
+345
+00:25:52,150 --> 00:25:59,430
+مستوى مستوى مستوى مستوى مستوى مستوى مستوى مستوى
+
+346
+00:25:59,430 --> 00:26:08,810
+مستوى مستوى مستوى مستو
+
+347
+00:26:09,020 --> 00:26:14,820
+في وقت معين، كل وقت معين بيتضاعف اللي هو العدد
+
+348
+00:26:14,820 --> 00:26:21,280
+الخلايا البكتيرية بمعدل ثابت. الـ
+
+349
+00:26:21,280 --> 00:26:25,360
+factors اللي بتأثر على النمو بشكل عام في الـ slides
+
+350
+00:26:25,360 --> 00:26:29,720
+القبل، كان في عوامل بتأثر على مثلا الـ log phase، على
+
+351
+00:26:29,720 --> 00:26:32,980
+الـ lag phase، على الـ stationary phase، لكن هنا
+
+352
+00:26:32,980 --> 00:26:36,880
+بنتكلم على النمو بشكل عام. أول شيء درجة الحرارة، أكيد
+
+353
+00:26:36,880 --> 00:26:40,630
+في بكتيريا بتنمو على درجات حرارة عالية، في بكتيريا
+
+354
+00:26:40,630 --> 00:26:44,610
+بتنمو على درجات حرارة منخفضة، معظم البكتيريا بتنمو
+
+355
+00:26:44,610 --> 00:26:49,370
+على درجات حرارة متوسطة، زي الـ 37 درجة سيلسيوس. الـ
+
+356
+00:26:49,370 --> 00:26:54,550
+pH كمان، الـ pH عندي طبعا بيختلف من بكتيريا
+
+357
+00:26:54,550 --> 00:26:58,070
+لبكتيريا. في بكتيريا بتنمو at alkaline pH على سبيل
+
+358
+00:26:58,070 --> 00:27:01,690
+المثال، الـ Vibrio cholerae، وفي اللي هو الـ neutral
+
+359
+00:27:01,690 --> 00:27:06,930
+pH، وفي الـ acidic pH، فبيختلف من بكتيريا لأخرى. ثاني
+
+360
+00:27:06,930 --> 00:27:10,610
+عامل، الـ salt concentration، أكيد في بكتيريا
+
+361
+00:27:10,610 --> 00:27:15,710
+بتتحمل الملوحة العالية، بتتحمل 7.5% NaCl زي ما
+
+362
+00:27:15,710 --> 00:27:21,390
+ذكرنا، اللي هي عائلة الـ staphylococcus، تمام؟ في بتتحمل
+
+363
+00:27:21,390 --> 00:27:26,550
+درجة 6.5% من اللي هو 6.5% NaCl زي الـ Enterobacter،
+
+364
+00:27:26,550 --> 00:27:32,310
+لكن باقي أنواع البكتيريا اللي هي بتتحمل 85% من 100
+
+365
+00:27:32,310 --> 00:27:36,710
+% NaCl. آخر شيء الـ oxygen concentration وهذا
+
+366
+00:27:36,710 --> 00:27:42,110
+شرحناه بـ chapter كامل، اتكلمنا عن كل التفاصيل اللي
+
+367
+00:27:42,110 --> 00:27:49,890
+هو في chapter الـ oxygen requirement. في
+
+368
+00:27:49,890 --> 00:27:53,490
+هذه الـ slide، هنتكلم كيف بدنا نقيس الـ microbial
+
+369
+00:27:53,490 --> 00:27:57,970
+growth، كيف بدي أقيس مدى النمو؟ راح أشوف عكارة، هيكون
+
+370
+00:27:57,970 --> 00:28:03,000
+فيها، دي turbidity، عكارة. رح أقيسها من خلال جهاز
+
+371
+00:28:03,000 --> 00:28:07,440
+spectrophotometer، هقرأ الـ absorbance. طبعا هيكون
+
+372
+00:28:07,440 --> 00:28:10,400
+في عندي light source، هيمر الضوء عبر اللي هي الـ
+
+373
+00:28:10,400 --> 00:28:14,500
+sample، هيكون في عندي turbidity، عكارة، هيصير في عندي
+
+374
+00:28:14,500 --> 00:28:19,260
+امتصاص اللي هو الـ absorbance، و بالنهاية هقرأ الـ
+
+375
+00:28:19,260 --> 00:28:24,580
+absorbance لكل اللي هي في خلال كل وقت معين.
+
+376
+00:28:28,270 --> 00:28:31,950
+بالنسبة للأدوات اللي هستخدمها في هذه التجربة، أول
+
+377
+00:28:31,950 --> 00:28:35,630
+شيء اللي هي الـ tryptic soy broth، هاي ميديا سائلة، هيكون
+
+378
+00:28:35,630 --> 00:28:40,690
+مزرعة فيها non-pathogenic E.coli، هتكون كـ stock
+
+379
+00:28:40,690 --> 00:28:45,210
+culture، عشان آخد منها inoculum E.coli، وأزرع في
+
+380
+00:28:45,210 --> 00:28:50,230
+ميديا أخرى، inoculating loop، عشان أزرع البكتيريا من
+
+381
+00:28:50,230 --> 00:28:54,950
+الـ stock culture، spectrophotometer عشان أقرأ الـ
+
+382
+00:28:54,950 --> 00:28:59,040
+absorbance، test tubes، راك، بدي راك عشان أحط الـ
+
+383
+00:28:59,040 --> 00:29:06,380
+tubes فيه، two tubes of tryptic soy broth، واحدة
+
+384
+00:29:06,380 --> 00:29:11,980
+هتكون للـ blank عشان أصفّر عليها، التانية عشان أزرع
+
+385
+00:29:11,980 --> 00:29:16,480
+فيها البكتيريا، للـ test. هاخد من اللي هي الـ stock
+
+386
+00:29:16,480 --> 00:29:21,200
+culture اللي موجود فيها E.coli، وهزرع في tube أخرى
+
+387
+00:29:21,200 --> 00:29:24,020
+tryptic soy broth اللي هتكون مش موجود فيها
+
+388
+00:29:24,020 --> 00:29:27,890
+البكتيريا. أكيد هيكون موجود في عندي أو بيلزمني
+
+389
+00:29:27,890 --> 00:29:32,150
+incubator عشان أنمي البكتيريا اللي عندي أنا.
+
+390
+00:29:32,150 --> 00:29:35,570
+بالنهاية بدي أشوف الـ microbial growth، معدل نمو
+
+391
+00:29:35,570 --> 00:29:41,050
+البكتيريا. bunsen burner، بما أني هزرع بكتيريا، فأكيد لازم أزرع
+
+392
+00:29:41,050 --> 00:29:46,250
+في ظروف sterile، surface disinfectant. هذا بالنسبة
+
+393
+00:29:46,250 --> 00:29:48,790
+للأدوات اللي هستخدمها في هذه التجربة.
+
+394
+00:29:55,850 --> 00:29:59,530
+بالنسبة للخطوات، أول شيء هغسل أيدي، ألبس الـ gloves،
+
+395
+00:29:59,530 --> 00:30:04,650
+أجهز أدواتي ومنطقة الشغل، هشغل spectrophotometer، و
+
+396
+00:30:04,650 --> 00:30:1
+
+445
+00:33:45,310 --> 00:33:49,310
+اتنين بعد خمس دقائق، طبعا عند عشر دقائق يكون وقتها
+
+446
+00:33:49,310 --> 00:33:54,150
+المدة كانت ثلاثة، وهكذا، هاخد طبعا قراءات، هعمل اللي
+
+447
+00:33:54,150 --> 00:33:59,020
+هو محاور لـ absorbance والـ time، بعد ما اخلص طبعا
+
+448
+00:33:59,020 --> 00:34:07,240
+هنظف المكان، وهشيل gloves، واغسل ايديا، هبدأ
+
+449
+00:34:07,240 --> 00:34:12,120
+أسجل اللي هو الـ absorbance لكل وقت معين حسب الجدول
+
+450
+00:34:12,120 --> 00:34:16,110
+من الملاحظ أنه عند كل وقت معين هيكون فيه قيمة
+
+451
+00:34:16,110 --> 00:34:21,110
+absorbance مختلفة، هعمل بالنهاية XY Axis، هيكون لـ
+
+452
+00:34:21,110 --> 00:34:24,630
+لـ absorbance و لـ time، هعمل الـ chart بالنهاية
+
+453
+00:34:24,630 --> 00:34:28,550
+هيوضح اللي هو الـ Growth Curve، الـ Lag Phase، الـ
+
+454
+00:34:28,550 --> 00:34:32,930
+Log Phase، والـ Stationary Phase، ليش متذكر الـ Death
+
+455
+00:34:32,930 --> 00:34:35,610
+Phase؟ لأنه إحنا قولنا في الـ Turbidometric
+
+456
+00:34:35,610 --> 00:34:40,770
+Measurement ما بقدر ألاحظ الـ Death Phase لأن الـ
+
+457
+00:34:40,770 --> 00:34:44,450
+Count بيكون لـ الـ Death Cell والـ Viable Cell مش
+
+458
+00:34:44,450 --> 00:34:47,770
+هقدر أميزهم عن بعض في الـ Microscopic Count وفي
+
+459
+00:34:47,770 --> 00:34:51,370
+الـ Turbidometric Measurement، لكن في الـ Plate
+
+460
+00:34:51,370 --> 00:34:53,170
+Count بقدر أميز
+
+461
+00:34:58,830 --> 00:35:04,370
+هذا الـ curve موضح المراحل النمو فيه أو نستنتج فيه
+
+462
+00:35:04,370 --> 00:35:10,650
+صورة، يبدو أن هذا صراحة أفضل من الـ curve اللي مـ
+
+463
+00:35:10,650 --> 00:35:15,150
+معناه، أول شيء في الـ lag phase مافيش عندي خلايا في
+
+464
+00:35:15,150 --> 00:35:19,030
+الـ log phase اللي هو في عندي عدد الخلايا الحية
+
+465
+00:35:19,030 --> 00:35:22,970
+أكثر من عدد الخلايا الميتة، إذا مانلاحظ تحت مكتوب
+
+466
+00:35:22,970 --> 00:35:27,030
+أن الـ death cell بكون لونها أسود، الـ viable cell في
+
+467
+00:35:27,030 --> 00:35:31,210
+ND زي نقاط كتيرة، لكن في أول مرحلة بيكون فيه ND few
+
+468
+00:35:31,210 --> 00:35:34,550
+cells اللي هي النقاط القليلة في الـ lag phase
+
+469
+00:35:34,550 --> 00:35:40,710
+منلاحظ أن الـ viable cell أكثر من الـ death cell في
+
+470
+00:35:40,710 --> 00:35:44,290
+الـ stationary phase، عدد الخلايا الميتة بيساوي عدد
+
+471
+00:35:44,290 --> 00:35:48,190
+الخلايا الحية، في الـ death phase منلاحظ أن عدد
+
+472
+00:35:48,190 --> 00:35:54,140
+الخلايا الميتة أكثر من عدد الخلايا الحية، طبعا هذا
+
+473
+00:35:54,140 --> 00:35:57,960
+الـ curve هو المظبوط أكثر من الـ curve اللي مـ
+
+474
+00:35:57,960 --> 00:36:03,240
+معناه لأنه موضح كل المراحل، أو بيبين عدد الخلايا
+
+475
+00:36:03,240 --> 00:36:04,200
+بشكل أفضل
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/PYxwZLDpRfI_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/PYxwZLDpRfI_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..c8ce12abdccdcc120c50534e0ef39db6ce23661f
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/PYxwZLDpRfI_postprocess.srt
@@ -0,0 +1,1900 @@
+1
+00:00:03,040 --> 00:00:06,920
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:06,920 --> 00:00:11,440
+جميعا نستكمل معاكم محاضرات مساق الـ Microbiology
+
+3
+00:00:11,440 --> 00:00:16,620
+العملي وفي آخر محاضرة في هذا المساق هنتكلم في هذه
+
+4
+00:00:16,620 --> 00:00:20,240
+المحاضرة عن موضوعين الموضوع الأول بكتيريا
+
+5
+00:00:20,240 --> 00:00:24,540
+generation time generation time هو الوقت اللازم
+
+6
+00:00:24,540 --> 00:00:29,410
+البكتيريا حتى تنقسم وتضاعفبالنسبة للموضوع التاني
+
+7
+00:00:29,410 --> 00:00:34,750
+Microbial Control Agent العوامل اللي بتتحكم في نمو
+
+8
+00:00:34,750 --> 00:00:48,110
+الميكروبات إما بالتثبيت أو بالقتل Growth
+
+9
+00:00:48,110 --> 00:00:53,430
+النمو، إيش هو النمو؟ is an orderly increase in the
+
+10
+00:00:53,430 --> 00:00:58,610
+quantity of cellular constituentsالنمو هو زيادة في
+
+11
+00:00:58,610 --> 00:01:04,550
+محتويات الخلية البكتيرية زيادة في الـ Biomass في
+
+12
+00:01:04,550 --> 00:01:09,350
+الكتلة الحيوية للخلية النمو على إيش بيعتمد It
+
+13
+00:01:09,350 --> 00:01:12,890
+depends upon the ability of the cell to form new
+
+14
+00:01:12,890 --> 00:01:16,330
+protoplasm from nutrients available in the
+
+15
+00:01:16,330 --> 00:01:21,050
+environment بيقول بيعتمد على قدرة الخلية إنها
+
+16
+00:01:21,050 --> 00:01:26,850
+اتكون protoplasm جديد protoplasm يعني cytoplasmمن
+
+17
+00:01:26,850 --> 00:01:31,010
+المواد الغذائية الموجودة في البيئة المحيطة بالخلية
+
+18
+00:01:31,010 --> 00:01:36,010
+البكتيريا بقولي المواد البكتيريا بعض البكتيريا الـ
+
+19
+00:01:36,010 --> 00:01:40,690
+growth إنه هو بيعتمد على increase أو بيضم increase
+
+20
+00:01:40,690 --> 00:01:44,830
+in cell mass قولنا ال growth هو زيادة في الكتلة
+
+21
+00:01:44,830 --> 00:01:49,030
+الحيوية مش بس زيادة في الكتلة الحيوية بقولي كمان
+
+22
+00:01:49,030 --> 00:01:54,590
+and number of ribosomes زيادة اللي هو ال ribosomes
+
+23
+00:01:54,590 --> 00:01:58,130
+عدد ال ribosomesduplication of the bacterial
+
+24
+00:01:58,130 --> 00:02:02,110
+chromosome أكيد هيصير في عندي تضاعف للمادة
+
+25
+00:02:02,110 --> 00:02:07,490
+الوراثية للبكتيريا لأنه بنهاية هيتكون عندي خليتين
+
+26
+00:02:07,490 --> 00:02:11,610
+بكتيريتين كل بكتيرية هتاخد اي chromosome synthesis
+
+27
+00:02:11,610 --> 00:02:15,770
+of a new cell wall and plasma membrane برضه هيتكون
+
+28
+00:02:15,770 --> 00:02:19,150
+cell wall جديد و plasma membrane جديد لأنه احنا
+
+29
+00:02:19,150 --> 00:02:25,600
+قلنا هيتكون عنديto cell فبالتالي كل خلية بكتيرية
+
+30
+00:02:25,600 --> 00:02:31,100
+هيكون لها cell wall منفصل plasma membrane منفصل
+
+31
+00:02:31,100 --> 00:02:37,320
+مكونات chromosome منفصل كمان بقولي partitioning of
+
+32
+00:02:37,320 --> 00:02:43,100
+the two chromosomes هيصير في عندي انه انقسام لـ
+
+33
+00:02:43,100 --> 00:02:46,760
+chromosomes ل two chromosomes لأن كل خلية بكتيرية
+
+34
+00:02:46,760 --> 00:02:51,290
+هتاخد chromosomeSeptum Formation مايعني سبتم
+
+35
+00:02:51,290 --> 00:02:55,950
+فورماشن يعني اللي هو هيتكون عندي حاجز لما يصير قبل
+
+36
+00:02:55,950 --> 00:02:59,110
+ما يصير في عندي cell division هيصير في عندي تكوين
+
+37
+00:02:59,110 --> 00:03:04,270
+حاجز تمام بعدها هيصير عندي الانقسام هنشوف في ال
+
+38
+00:03:04,270 --> 00:03:08,890
+slide اللي بعيدها شو هو هذا الحاجز بالنهاية هتنقسم
+
+39
+00:03:08,890 --> 00:03:12,530
+الخلية البكتيرية لخليتين بكتيريتين هيصير في عندي
+
+40
+00:03:12,530 --> 00:03:17,040
+cell divisionاللي هو الانقسام بواسطة الـ Binary
+
+41
+00:03:17,040 --> 00:03:21,360
+Evasion اللي هو الانشطار الثنائي شو هو الانشطار
+
+42
+00:03:21,360 --> 00:03:24,940
+الثنائي؟ بقول لي this a sexual process of
+
+43
+00:03:24,940 --> 00:03:28,360
+reproduction is called binary evasion اللي هي
+
+44
+00:03:28,360 --> 00:03:34,480
+بتكون عملية لا جنسية مش sexual، a sexual process
+
+45
+00:03:34,480 --> 00:03:39,400
+لأنه بالنهاية أنا بتكون أو الانقسام بيصير من خلية
+
+46
+00:03:39,400 --> 00:03:44,890
+واحدة من mother cells مش من خليتينمش من أب و أم
+
+47
+00:03:44,890 --> 00:03:49,950
+فبالتالي هي لاجنسية عملية لاجنسية asexual process
+
+48
+00:03:49,950 --> 00:03:53,470
+اللي هي ال binary effusion اللي هي الانشطار
+
+49
+00:03:53,470 --> 00:03:58,230
+الثنائي كيف
+
+50
+00:03:58,230 --> 00:04:02,790
+بيصير ال binary division أو ال cell division لخلية
+
+51
+00:04:02,790 --> 00:04:06,250
+البكتيريا نلاحظ في الصورة أن أنا في عندي خلية
+
+52
+00:04:06,250 --> 00:04:12,410
+بكتيرية فيها ال DNA المادة الوراثيةالـ DNA هيصير
+
+53
+00:04:12,410 --> 00:04:16,310
+له اللي هو تضاعف عندي هيصير DNA replication نلاحظ
+
+54
+00:04:16,310 --> 00:04:20,490
+في الصورة إن والله اتضاعف اللي هو الـ Circular DNA
+
+55
+00:04:20,490 --> 00:04:25,550
+طبعا خلية البكتيريا قلنا في أول معمل هو عبارة عن
+
+56
+00:04:25,550 --> 00:04:30,670
+Circular دائري مش linear زي الإنسان هيصير في عندي
+
+57
+00:04:30,670 --> 00:04:35,220
+DNA replication تضاعف للـ DNAهيصير فيه cell
+
+58
+00:04:35,220 --> 00:04:39,840
+elongation هيصير استطالة لخلية البكتيريا بعد كده
+
+59
+00:04:39,840 --> 00:04:44,300
+البكتيريا هتحضر حالها للانقسام هيصير في عينيه
+
+60
+00:04:44,300 --> 00:04:48,860
+يقولنا تكون حاجز symptom formation اللي هو يتكون
+
+61
+00:04:48,860 --> 00:04:54,780
+في عينيه حاجز حتى إن الخلية البكتيرية تحضر حالها
+
+62
+00:04:54,780 --> 00:05:00,580
+للانقسام لخليتين بشكل كامل وتبتعد عن بعض بنلاحظ في
+
+63
+00:05:00,580 --> 00:05:06,030
+الصورة هنا إنهصار في عندي اللي هو انفصال لخليتين
+
+64
+00:05:06,030 --> 00:05:09,890
+البكتيريتين عن بعض اللي هو الـ two daughter cell
+
+65
+00:05:09,890 --> 00:05:14,170
+separated بعدها صار في عندي اللي هو كل خلية
+
+66
+00:05:14,170 --> 00:05:19,130
+بكتيرية انفصلت صار لها DNA خاص فيها وزي ما ملاحظ
+
+67
+00:05:19,130 --> 00:05:24,150
+عبارة عن اللي هو Circular دائري طبعاً لها cell
+
+68
+00:05:24,150 --> 00:05:27,930
+wall خاص فيها cytoplasm خاص فيها cell membrane خاص
+
+69
+00:05:27,930 --> 00:05:31,070
+فيها chromosome خاص فيها زي ما اتكلمنا في ال slide
+
+70
+00:05:31,070 --> 00:05:39,940
+اللي قبلهذا بالنسبة لـ Cell Division في
+
+71
+00:05:39,940 --> 00:05:44,340
+هاي الـ slide هنتعرف على بعض المصطلحات الهمة أول
+
+72
+00:05:44,340 --> 00:05:47,820
+مصطلح اللي هو الـ Growth بالنسبة لـ Growth عرفناه
+
+73
+00:05:47,820 --> 00:05:51,940
+في الـ slide الأبل وقلنا اللي هو زيادة في الكتلة
+
+74
+00:05:51,940 --> 00:05:56,460
+الحيويةThe Equation of Biomass اللي هو زيادة في
+
+75
+00:05:56,460 --> 00:06:00,240
+الكتلة الحيويةleading to cell revision بالنهاية
+
+76
+00:06:00,240 --> 00:06:04,920
+هيصير عندي انقسام لالخلية لخليتين بكتيريتين or
+
+77
+00:06:04,920 --> 00:06:09,920
+reproduction تاني مصطلح a batch culture ال batch
+
+78
+00:06:09,920 --> 00:06:14,200
+culture is a closed system نظام مغلق in a broth
+
+79
+00:06:14,200 --> 00:06:18,020
+medium in which no additional nutrient واللي هو
+
+80
+00:06:18,020 --> 00:06:22,040
+نظام مغلق في ال broth media مايصير في عندي أي
+
+81
+00:06:22,040 --> 00:06:26,550
+إضافات للمواد الغذائيةBad is added after
+
+82
+00:06:26,550 --> 00:06:30,230
+incubation of the brood بعد ما أنا أزرع هذه
+
+83
+00:06:30,230 --> 00:06:34,610
+البكتيريا يعني بكون عندي ميديا وبزرع فيها
+
+84
+00:06:34,610 --> 00:06:40,610
+البكتيريا وبحطها في ال incubator لو خلصت المغذيات
+
+85
+00:06:40,610 --> 00:06:44,050
+و البكتيريا في ال incubator أو بعد ما أنا طلعت هذا
+
+86
+00:06:44,050 --> 00:06:48,630
+الطبق من ال incubator بتموت البكتيريا لكن هذا
+
+87
+00:06:48,630 --> 00:06:52,800
+بالنسبة لبatch culture في النظام المغلقفي النظام
+
+88
+00:06:52,800 --> 00:06:57,320
+المغلق أنا ما بضيف أي مغذيات بعد اللي هو الزراعة
+
+89
+00:06:57,320 --> 00:07:02,460
+خلاص إذا خلصت المغذيات البكتيريا بتموت لكن بالنسبة
+
+90
+00:07:02,460 --> 00:07:06,340
+لـ continuous culture في عندي أنا نظام مفتوح حد
+
+91
+00:07:06,340 --> 00:07:10,580
+نظام كل ما اتخلص المواد الغذائية أنا بضيف غيرها
+
+92
+00:07:10,580 --> 00:07:15,960
+فبالتالي في نظام ال continuous culture ما بتمر
+
+93
+00:07:15,960 --> 00:07:20,510
+البكتيريا بمرحلة اللي هو ال death phaseفي مرحلة
+
+94
+00:07:20,510 --> 00:07:24,150
+الموت لأني أنا بصير أضيف مواد غذائية لكن في الـ
+
+95
+00:07:24,150 --> 00:07:27,830
+Closed System اللي هو الـ Batch Culture ما بضيف أي
+
+96
+00:07:27,830 --> 00:07:32,350
+مغذية No additional nutrient بعد الزراعة فبالتالي
+
+97
+00:07:32,350 --> 00:07:36,290
+البكتيريا بتموت و بتمر بمرحلات اللي هو الـ Death
+
+98
+00:07:36,290 --> 00:07:41,250
+phase بالنسبة للـ Generation Time اتكلمنا عنه Time
+
+99
+00:07:41,250 --> 00:07:44,510
+taken for a cell population to double in number هو
+
+100
+00:07:44,510 --> 00:07:49,200
+الوقت اللي لازم لاقى الخلية انها تتضاعف في العددهو
+
+101
+00:07:49,200 --> 00:07:52,600
+بقول اللي بيساوي the average length of the cell
+
+102
+00:07:52,600 --> 00:07:59,120
+cycle هذا بالنسبة لهذه المصطلحات اللي هتمر معناها
+
+103
+00:07:59,120 --> 00:08:05,340
+خلال الـ chapter الـ
+
+104
+00:08:05,340 --> 00:08:09,680
+bacterial growth curve اللي هو منحنة نمو البكتيريا
+
+105
+00:08:09,680 --> 00:08:14,700
+في عندي أربع أطوار أو أربع stage بتمر فيهم الخلية
+
+106
+00:08:14,700 --> 00:08:18,910
+البكتيرية في النموfour characteristic phase of the
+
+107
+00:08:18,910 --> 00:08:22,910
+growth cycle اللي هم ال lag phase ال log phase ال
+
+108
+00:08:22,910 --> 00:08:27,050
+stationary phase ال death phase هنتكلم عن كل phase
+
+109
+00:08:27,050 --> 00:08:31,070
+بالتفصيل أول phase اللي هو ال lag phase اللي هو
+
+110
+00:08:31,070 --> 00:08:35,650
+التطور التحضيري للانقسام هذا التطور بنعطي فرصة
+
+111
+00:08:35,650 --> 00:08:38,970
+للبكتيريا إنها تتأقلم إنه يصير في عندي adaptation
+
+112
+00:08:38,970 --> 00:08:44,130
+تحضر حالها للانقسام تحضر الانزيمات للانقساملكن
+
+113
+00:08:44,130 --> 00:08:47,370
+فعليًا ما بيصير في عندي أي نمو ما بيصير عندي أي
+
+114
+00:08:47,370 --> 00:08:52,630
+انقسام ما بيصير عندي زيادة في العدد متى هذا ال
+
+115
+00:08:52,630 --> 00:08:56,350
+fest بيصير immediately after the incubation of the
+
+116
+00:08:56,350 --> 00:09:00,190
+cell into fresh media بقوللي وقت ما أنتي تزرعي
+
+117
+00:09:00,190 --> 00:09:04,390
+الخلايا في ال fresh media هانبتبدأ مرحلة ال lag
+
+118
+00:09:04,390 --> 00:09:08,350
+fest ال population بقوللي ال population remained
+
+119
+00:09:08,350 --> 00:09:13,670
+temporarily unchanged بقوللي ما بيصير في عنديأي
+
+120
+00:09:13,670 --> 00:09:17,790
+تغيير في العدد تمام ما بيصير أي تغيير في العدد
+
+121
+00:09:17,790 --> 00:09:22,030
+بشكل مؤقت although there is no apparent cell
+
+122
+00:09:22,030 --> 00:09:25,050
+division بقولي ما بيصير في عندي أي انقسام اتفاقنة
+
+123
+00:09:25,050 --> 00:09:28,570
+ما بيصير في عندي أي نمو the cell may be growing in
+
+124
+00:09:28,570 --> 00:09:33,230
+volume لكن ممكن الخلايا يصير في عندي زيادة في
+
+125
+00:09:33,230 --> 00:09:37,430
+الحجم ال volume وليس العدد ما بيصير في عندي زيادة
+
+126
+00:09:37,430 --> 00:09:42,000
+في العدد قولنا ال population بيظلواطبعاً بشكل مؤقت
+
+127
+00:09:42,000 --> 00:09:46,040
+ثابت ما بتغير لكن بقولي ممكن يصير في عندي زيادة في
+
+128
+00:09:46,040 --> 00:09:50,280
+الحجم في ال biomass لكن مش في العدد بيصير في عندي
+
+129
+00:09:50,280 --> 00:09:54,240
+synthesizing لإنزايم أكيد بتحضر حالها لإنقسام
+
+130
+00:09:54,240 --> 00:10:00,300
+فبالتالي بيتم إنتاج إنزيمات بروتين RNA increasing
+
+131
+00:10:00,300 --> 00:10:04,700
+in metabolic activity كل هذه الأمور بتصير عشان
+
+132
+00:10:04,700 --> 00:10:09,180
+تحضر حالها لإنقسام لكن ما بيصير في عندي أي نمو
+
+133
+00:10:17,300 --> 00:10:21,120
+هذه الرسمة بتعبر عن مراحل نمو البكتيريا قلنا فيه
+
+134
+00:10:21,120 --> 00:10:26,000
+عندي four stages lag-log stationary death phase
+
+135
+00:10:26,000 --> 00:10:31,000
+أول مرحلة أو أول stage اللي هو الـ lag phase قلنا
+
+136
+00:10:31,000 --> 00:10:36,380
+إنه عدد البكتيريا في هذا الطور بيظل ثابت un
+
+137
+00:10:36,380 --> 00:10:41,740
+-exchanged طبعا أنا عندي في هذا المحور اللي هو
+
+138
+00:10:41,740 --> 00:10:45,640
+الـtime وهذا المحور عدد البكتيريا عدد البكتيريا في
+
+139
+00:10:45,640 --> 00:10:50,470
+الـ lag phaseبيظلوا ما بيتغير بيظلوا ثابت زي ما
+
+140
+00:10:50,470 --> 00:10:54,150
+بنلاحظ في الصورة انه العدد ثابت في مرحلة ال lag
+
+141
+00:10:54,150 --> 00:10:58,550
+phase بقوللي the length of the lag phase is
+
+142
+00:10:58,550 --> 00:11:03,010
+apparently dependent on a wide variety of factors
+
+143
+00:11:03,010 --> 00:11:08,210
+including شو العوامل اللي بتأثر على طول ال lag
+
+144
+00:11:08,210 --> 00:11:13,810
+phase ممكن مثلا في بكتيريا يكون ال lag phase50 أو
+
+145
+00:11:13,810 --> 00:11:18,850
+زي ما ملاحظ فيها الصورة يعني تقريباً 20 minute في
+
+146
+00:11:18,850 --> 00:11:22,490
+بكتيريا تانية ممكن يكون مثلاً خمسين minute أو
+
+147
+00:11:22,490 --> 00:11:27,350
+تلاتين minute فبيختلف طول ال lag phase من بكتيريا
+
+148
+00:11:27,350 --> 00:11:31,430
+لبكتيريا فيلم دي كتير عوامل أو عمامل بتأثر على
+
+149
+00:11:31,430 --> 00:11:35,950
+اللي هو طول ال lag phase طول مدة ال lag phase هلأ
+
+150
+00:11:35,950 --> 00:11:39,820
+بالنسبة لل generation timeالـ generation time لكل
+
+151
+00:11:39,820 --> 00:11:45,880
+بكتيريا مختلف مش نفس الشيء في كل البكتيريات طبعا
+
+152
+00:11:45,880 --> 00:11:50,100
+احنا ذكرنا لما شرحنا ال acid fast stain اللي هي
+
+153
+00:11:50,100 --> 00:11:53,760
+البكتيريا ال micro bacterium tuberculosis بتحتاج
+
+154
+00:11:53,760 --> 00:12:00,200
+20 ساعة عشان تنمو في نوع بكتيريا اخر خلال مثلا ربع
+
+155
+00:12:00,200 --> 00:12:04,080
+ساعة تلت ساعة بتنمو البكتيريا فبالتالي ال
+
+156
+00:12:04,080 --> 00:12:07,100
+generation time الوقت اللي لازم لنمو البكتيريا
+
+157
+00:12:07,100 --> 00:12:12,040
+بيختلفمن بكتيريا لأخرى ال lag phase نفس الشيء مش
+
+158
+00:12:12,040 --> 00:12:15,920
+كل البكتيريا بتتكيف بنفس الدرجة في عندي factor
+
+159
+00:12:15,920 --> 00:12:21,520
+بتأثر على طول ال lag phase أول factor the size of
+
+160
+00:12:21,520 --> 00:12:26,900
+the inicula اللي هو قداش أنا أخدت بكتيريا وزرعتها
+
+161
+00:12:26,900 --> 00:12:30,940
+في ال growth media حجم ال iniculaطبعا أكيد
+
+162
+00:12:30,940 --> 00:12:34,540
+البكتيريا الأليلة هتحضر حالها بشكل أسرع لكن
+
+163
+00:12:34,540 --> 00:12:39,160
+البكتيريا لو أنا أخدت انيكولام وكان الحجم أكتر رح
+
+164
+00:12:39,160 --> 00:12:43,540
+تاخد وقت أكتر لكن البكتيريا لو أنا أخدت انيكولام
+
+165
+00:12:43,540 --> 00:12:49,060
+وكان الحجم أليل أكيد رح تحضر حالها بشكل أسرع يبقى
+
+166
+00:12:49,060 --> 00:12:53,740
+هذا بالنسبة لأول factor بيأثر على ال lag fast تاني
+
+167
+00:12:53,740 --> 00:12:59,110
+factor timeالذي يجب أن يتجاوز من المصادر الواقعية
+
+168
+00:12:59,110 --> 00:13:05,050
+أو من المصادر الواقعية بقول لي الوقت اللازم حتى أن
+
+169
+00:13:05,050 --> 00:13:08,790
+البكتيريا تتكيف في حال صار في عندها صدمة خلال
+
+170
+00:13:08,790 --> 00:13:13,790
+عملية النقل البكتيريا قبل ما أنا أزرعها في اللي هو
+
+171
+00:13:13,790 --> 00:13:16,990
+ال media أكيد بتكون في وسط ممكن تكون في تربة في
+
+172
+00:13:16,990 --> 00:13:21,690
+هواء في طعام فبالتالي خلال ال transfer ممكن تكون
+
+173
+00:13:21,690 --> 00:13:26,620
+اتعرضت ل shock ل physical damageالوقت اللي بتحتاجه
+
+174
+00:13:26,620 --> 00:13:30,660
+هذه البكتيريا عشان تتكيف مع الوسط اللي أنا وضعته
+
+175
+00:13:30,660 --> 00:13:34,100
+فيها مع ال media اللي أنا وضعتها فيها أو تعالج
+
+176
+00:13:34,100 --> 00:13:38,700
+نفسها من الأضرار واصضاماتها ده كله بيأثر على اللي
+
+177
+00:13:38,700 --> 00:13:43,720
+هو الطول ال lag fest تالت factor time required for
+
+178
+00:13:43,720 --> 00:13:48,360
+synthesis of essential coenzyme or division factor
+
+179
+00:13:48,580 --> 00:13:52,920
+الوقت اللازم إنها اتصنع الـ Coenzyme شو هو الـ
+
+180
+00:13:52,920 --> 00:13:56,560
+Coenzyme؟ هو الفاكتور اللي بيساعد على عمل الانزيم
+
+181
+00:13:56,560 --> 00:14:02,220
+كمحفزة تآخر فاكتور بيأثر على طول الـ lag phase
+
+182
+00:14:02,220 --> 00:14:06,260
+اللي هو الـ time required for synthesis of a new
+
+183
+00:14:06,260 --> 00:14:09,480
+enzyme that are necessary to metabolize the
+
+184
+00:14:09,480 --> 00:14:13,560
+substrate present in the media هو الوقت اللازم
+
+185
+00:14:13,560 --> 00:14:18,020
+لإنتاج انزيماتضرورية عشان يصير في هندي اللي هو
+
+186
+00:14:18,020 --> 00:14:22,280
+تساعدها أو تستعملها في العمليات الحيوية في الـ
+
+187
+00:14:22,280 --> 00:14:26,780
+metabolism و تشتغل على ال substrate الموجود في ال
+
+188
+00:14:26,780 --> 00:14:31,340
+media هذا بالنسبة لل factor اللي بيأثر على طول ال
+
+189
+00:14:31,340 --> 00:14:36,820
+lag phase ويقولنا إن ال lag phase اللي هو بيختلفمش
+
+190
+00:14:36,820 --> 00:14:40,640
+كل البكتيريا بتتكيف بنفس الدرجة نفس فكرة ال
+
+191
+00:14:40,640 --> 00:14:43,540
+generation type انه لكل بكتيريا بيكون فيها انديه
+
+192
+00:14:43,540 --> 00:14:49,040
+generation type مختلف عن البكتيريا الأخرى هذا
+
+193
+00:14:49,040 --> 00:14:52,200
+بالنسبة لهذا ال slide
+
+194
+00:14:57,950 --> 00:15:02,270
+بعد ما البكتيريا اتأقلمت وحضرت حالها راح تبدأ
+
+195
+00:15:02,270 --> 00:15:05,770
+بالانقسام بالـ cell division يعني هيصير في عندي
+
+196
+00:15:05,770 --> 00:15:11,050
+نمو وزيادة بالعدد بالـ number وهي مرحلة الـ log
+
+197
+00:15:11,050 --> 00:15:15,010
+phase أو الـ X potential phase الـ X potential
+
+198
+00:15:15,010 --> 00:15:19,490
+phase of growth is a pattern of balance growth ليش
+
+199
+00:15:19,490 --> 00:15:23,130
+قالي balance growth؟ لأن البكتيريا راح تبدأ تتضاعف
+
+200
+00:15:23,130 --> 00:15:27,740
+بمعدل ثابتيعني كل خمس دقايق على سبيل المثال لها
+
+201
+00:15:27,740 --> 00:15:32,440
+تتضعف البكتيريا الواحدة وتصير اتناء طبعا هذا بسميه
+
+202
+00:15:32,440 --> 00:15:37,660
+اللي هو الزيادة اللغارتمية or الأسية growing by
+
+203
+00:15:37,660 --> 00:15:43,380
+geometric progression هذا بالنسبة لـ Geometric
+
+204
+00:15:43,380 --> 00:15:48,080
+progression هو الزيادة الأسية واللغارتمية أن
+
+205
+00:15:48,080 --> 00:15:51,960
+البكتيريا بتتضعف بمعدل ثابت كل خمس دقايق بتتضعف
+
+206
+00:15:52,220 --> 00:15:55,200
+بتصير على سبيل المثال طبعا هي مش كل البكتيريا
+
+207
+00:15:55,200 --> 00:15:58,900
+بيكون كل خمس دقايق لأ بيختلف من البكتيريا لآخر على
+
+208
+00:15:58,900 --> 00:16:03,060
+سبيل المثال انه في كل خمس دقائق بتتضاعف وبتصير
+
+209
+00:16:03,060 --> 00:16:06,660
+اتنين ممكن بكتيريا أخرى كل عشر دقائق بتتضاعف
+
+210
+00:16:06,660 --> 00:16:11,300
+وبتصير اتنين لكن بالنهاية التضاعف بيصير بمعدل ثابت
+
+211
+00:16:11,300 --> 00:16:16,900
+بيكون زيادة ليجوريتمية أو أستية wherein all the
+
+212
+00:16:16,900 --> 00:16:21,490
+cells are dividingregularly by binary illusion
+
+213
+00:16:21,490 --> 00:16:25,290
+طبعا الخلايا تنقسم بشكل منتظم زي ما قلنا بمعدل
+
+214
+00:16:25,290 --> 00:16:31,490
+ثابت بالانشطار الثنائي the cells divide at a
+
+215
+00:16:31,490 --> 00:16:36,110
+constant rate قلنا ان الخلايا تنقسم بمعدل ثابت
+
+216
+00:16:36,110 --> 00:16:40,430
+وفهمنا شو يعني بمعدل ثابتبالتالي، يعتمد على
+
+217
+00:16:40,430 --> 00:16:48,110
+محتويات الميديا وضروف الانكيباشيا طبعا، النمو
+
+218
+00:16:48,110 --> 00:16:53,650
+يعتمد على محتويات الميديا وكذلك على ضروف
+
+219
+00:16:53,650 --> 00:16:58,790
+الانكيباشيا قيمة إكس بوتنشيل إكروث بكتيريال
+
+220
+00:16:58,790 --> 00:17:04,210
+كالتشارت إكسبرس كجنراشن تايم الجنراشن تايم اللي
+
+221
+00:17:04,210 --> 00:17:09,120
+عرفناه في أول المحاضرة وقلناه هو الوقت اللازملأن
+
+222
+00:17:09,120 --> 00:17:15,380
+البكتيريا تتضاعف هو نفس الـ X potential growth of
+
+223
+00:17:15,380 --> 00:17:20,720
+bacterial culture يبقى لو أنا حكيت generation time
+
+224
+00:17:20,720 --> 00:17:25,320
+يبقى أنا بعبر عن اللي هو الـ X potential فاس برضه
+
+225
+00:17:25,320 --> 00:17:30,220
+له اسم تاني اللي هو ال doubling time لأنه قلنا أن
+
+226
+00:17:30,220 --> 00:17:35,300
+البكتيريا بيصير لها تضاعف doubling يعني تضاعف ففي
+
+227
+00:17:35,300 --> 00:17:45,040
+هذاالفاس البكتيريا بتتضعف بمعدل ثابت بالنسبة
+
+228
+00:17:45,040 --> 00:17:50,520
+لتالت مرحلة اللي هي الستشانري فاس البكتيريا لما
+
+229
+00:17:50,520 --> 00:17:54,220
+تموت بتنتج فضلات بتنتج waste بعدها رح تبدأ
+
+230
+00:17:54,220 --> 00:17:58,600
+البكتيريا تموت لما أوصل لمرحلة أنه البكتيريا أو
+
+231
+00:17:58,600 --> 00:18:02,120
+عدد البكتيريا اللي بتنمو بسوء عدد البكتيريا اللي
+
+232
+00:18:02,120 --> 00:18:07,580
+بتموتبسمي هذه المرحلة مرحلة الثبات الاستشانري فاس
+
+233
+00:18:07,580 --> 00:18:12,400
+طبعا ال rate of growth معدل النمو في مرحلة الثبات
+
+234
+00:18:12,400 --> 00:18:17,020
+بيساوي سفر exponential growth cannot be continue
+
+235
+00:18:17,020 --> 00:18:20,840
+forever in a batch culture for example closed
+
+236
+00:18:20,840 --> 00:18:25,340
+system such as destitute or افلسف بقولي انه في ال
+
+237
+00:18:25,340 --> 00:18:30,140
+exponential growth ما بيستمر في ال batch culture
+
+238
+00:18:30,510 --> 00:18:34,470
+واتفقنا على هذه المعلومة لما شرحنا شو هي الـ Batch
+
+239
+00:18:34,470 --> 00:18:38,350
+Culture الـ Batch Culture قولنا هي نظام مغلق ما
+
+240
+00:18:38,350 --> 00:18:42,810
+بضيف مواد غذائية بعد الـ Inclusion فبالتالي لما
+
+241
+00:18:42,810 --> 00:18:47,490
+توصل البكتيريا لمرحلة أو لما ال media يصير فيها
+
+242
+00:18:47,490 --> 00:18:51,410
+نقص في المواد الغذائية تخلص المواد الغذائية أكيد
+
+243
+00:18:51,410 --> 00:18:58,490
+البكتيريا هتبدأ تموت فبالتاليهنا هوصل لمرحلة اللي
+
+244
+00:18:58,490 --> 00:19:02,470
+هو الـ stationary phase ومرحلة الـ death phase الـ
+
+245
+00:19:02,470 --> 00:19:07,190
+X potential إجراث مش هيظل مستمر لكن هذا بالنسبة لل
+
+246
+00:19:07,190 --> 00:19:10,570
+batch culture لكن في ال continuous culture اللي
+
+247
+00:19:10,570 --> 00:19:14,450
+قلنا أنا بضيف مواد غذائية فبالتالي الـ X potential
+
+248
+00:19:14,450 --> 00:19:19,810
+إجراث هيظل مستمر في ال continuous cultureبيكون
+
+249
+00:19:19,810 --> 00:19:23,330
+مافي عندي مرحلة اللي هو الـ death phase في الـ
+
+250
+00:19:23,330 --> 00:19:26,830
+continuous culture في الـ open system راح توقف لإن
+
+251
+00:19:26,830 --> 00:19:30,710
+مرحلة الثبات وبعدها راح أضيف مقذيات وترجع تلوم
+
+252
+00:19:30,710 --> 00:19:35,450
+بقوللي إنه في مرحلة الثبات قداش راح تبقى فيه
+
+253
+00:19:35,450 --> 00:19:38,650
+بكتيريا بيعتمد على عدة عوامل population growth is
+
+254
+00:19:38,650 --> 00:19:42,230
+limited by one of the three factors أول عامل اللي
+
+255
+00:19:42,230 --> 00:19:46,390
+هو استهلاك المواد الغذائية هل سيكون عندي نقص في
+
+256
+00:19:46,390 --> 00:19:53,030
+المقذيات؟تاني عمل اللي هو زيادة ال West زيادة ال
+
+257
+00:19:53,030 --> 00:19:56,730
+West نتيجة العمليات الأيضية و تراجعه ما هذا أكيد
+
+258
+00:19:56,730 --> 00:20:00,910
+هيأثر في اللي هو قداش راح تظل البكتيريا في
+
+259
+00:20:00,910 --> 00:20:07,330
+البكتيريا طبعا حية في Lithium آخر factor اللي هو
+
+260
+00:20:07,330 --> 00:20:12,570
+نقص المساحة نقص المساحة في الأنبوب نتيجة زيادةعدد
+
+261
+00:20:12,570 --> 00:20:16,390
+الخلايا يبطل فيها انديوسع اللي هو a lack of
+
+262
+00:20:16,390 --> 00:20:21,010
+biological space اللي هو نقص المساحة الحيوية بيبطل
+
+263
+00:20:21,010 --> 00:20:25,810
+فيها انديوسع فبالتالي هاي كل العوامل اللي بتأثر في
+
+264
+00:20:25,810 --> 00:20:30,150
+مرحلة ثبات أداش راح تبقى في بكتيريا بيتمد على هاي
+
+265
+00:20:30,150 --> 00:20:35,010
+العوامل بالنسبة لرابع مرحلة اللي هي ال death phase
+
+266
+00:20:35,010 --> 00:20:38,450
+if incubation continues after the population
+
+267
+00:20:38,450 --> 00:20:42,380
+reaches stationary phase قولي لوالـ Incubation ظل
+
+268
+00:20:42,380 --> 00:20:45,540
+مستمر بعد ما أوصل لمرحلة الـ stationary phase اللي
+
+269
+00:20:45,540 --> 00:20:49,760
+هو معدل إنه بيكون فيها ثابت a death phase follows
+
+270
+00:20:49,760 --> 00:20:55,040
+بعدها بتبدأ مرحلة الـ death phase in which the
+
+271
+00:20:55,040 --> 00:20:58,960
+viable cell population decline بقوللي إنه عدد
+
+272
+00:20:58,960 --> 00:21:02,260
+الخلايا اللي بتنمو في الـ death phase ها بتبدأ
+
+273
+00:21:02,260 --> 00:21:05,760
+اتقل في مرحلة الـ stationary قولنا إن الـ viable
+
+274
+00:21:05,760 --> 00:21:09,820
+cell بتساوي اللي هي الخلايا الحية بتساوي الخلايا
+
+275
+00:21:09,820 --> 00:21:13,820
+الميتةلكن في الـ death face الـ viable cells
+
+276
+00:21:13,820 --> 00:21:18,520
+الخلايا الحية بتبدأ اتقل هندقلي ملاحظة أنا قولتها
+
+277
+00:21:18,520 --> 00:21:22,880
+في chapter الـ Serial Dilution if counting by
+
+278
+00:21:22,880 --> 00:21:25,580
+terpidometric measurement اللي هو الـ
+
+279
+00:21:25,580 --> 00:21:30,560
+spectrophotometer or microscopic count the death
+
+280
+00:21:30,560 --> 00:21:34,600
+face cannot be observed قولنا إنه أنا ما بميز
+
+281
+00:21:34,600 --> 00:21:37,780
+الخلايا الحية والخلايا الميتة لو أنا عديت في ال
+
+282
+00:21:37,780 --> 00:21:41,600
+terpidometric measurementأو لو استخدمت الـ
+
+283
+00:21:41,600 --> 00:21:44,900
+Microscopic Count لو استخدمت الـ Microscope ما
+
+284
+00:21:44,900 --> 00:21:49,160
+بفرق بين الخلايا الحية والخلايا الميتة فقط في
+
+285
+00:21:49,160 --> 00:21:54,440
+طريقة اللي هي الـ Viable Cells اللي هي الـ Blood
+
+286
+00:21:54,440 --> 00:21:58,340
+Count هي اللي بميز الخلايا الحية والميتة هي اللي
+
+287
+00:21:58,340 --> 00:22:02,400
+بتعد فقط الـ Viable Cell ما بتعد الخلايا الميتة
+
+288
+00:22:02,400 --> 00:22:07,340
+لأنه الأكيد البكتيريا اللي ماتت مش هتنمو على الطبق
+
+289
+00:22:08,010 --> 00:22:11,150
+فبالتالي لو أنا استخدمت هدول الطريقتين مش هلاحظ
+
+290
+00:22:11,150 --> 00:22:15,210
+الـ death phase فقط في اللي هو الـ blood count هذه
+
+291
+00:22:15,210 --> 00:22:18,110
+الملاحظة طبعا و أنا ذكرتها في الـ cereal dilution
+
+292
+00:22:18,110 --> 00:22:22,450
+هنكمل during the death phase the number of viable
+
+293
+00:22:22,450 --> 00:22:27,630
+cell degrees قولنا إنه في الـ death phase الخلايا
+
+294
+00:22:27,630 --> 00:22:32,750
+الحية بتبدأ تقل بشكل اللي هو logarithmicتمام نفس
+
+295
+00:22:32,750 --> 00:22:36,170
+اللي هو في الـ X potential الـ growth قلنا بيبدأ
+
+296
+00:22:36,170 --> 00:22:40,610
+يزيد عدد الخلايا بشكل لغرتمي بشكل قصي لكن هان
+
+297
+00:22:40,610 --> 00:22:45,790
+بيبدأ يقل بشكل لغرتمي essentially the reverse of
+
+298
+00:22:45,790 --> 00:22:49,070
+the growth during the log phase فقلي بيكون في شكل
+
+299
+00:22:49,070 --> 00:22:53,010
+عكسي لل log phase لأن ال log phase بيزيد بشكل
+
+300
+00:22:53,010 --> 00:22:58,410
+لغرتمي لكن بالنسبة لل death phase بيقل بشكل لغرتمي
+
+301
+00:22:58,820 --> 00:23:04,900
+هكذا خلصنا مراحل نمو البكتيريا وشرحنا كل الـ stage
+
+302
+00:23:04,900 --> 00:23:10,160
+اللي بتمر فيها البكتيريا من مرحلة الـ lag phase
+
+303
+00:23:10,160 --> 00:23:13,520
+الـ log phase الـ stationary phase و آخر الشيء
+
+304
+00:23:13,520 --> 00:23:17,140
+اللي هو الـ death phase طبعا هذا كله في ال batch
+
+305
+00:23:17,140 --> 00:23:21,660
+culture هاني
+
+306
+00:23:21,660 --> 00:23:26,190
+موضح مراحل نمو البكتيريا في صورةقلنا في عندي أربع
+
+307
+00:23:26,190 --> 00:23:30,010
+Stages اللي هو الـ Lag phase اللي هو اللي أنا من
+
+308
+00:23:30,010 --> 00:23:34,810
+وقت ما زرعت اللي هو الانيكولام في الـ media في الـ
+
+309
+00:23:34,810 --> 00:23:39,310
+fresh media بعدها هتبدأ الخلايا تنمو يصير في عندي
+
+310
+00:23:39,310 --> 00:23:43,150
+viable cell اللي هي الخلايا الحية في الـ X
+
+311
+00:23:43,150 --> 00:23:47,710
+potential phase بتبدأ يزيد عدد الخلايا الحية في
+
+312
+00:23:47,710 --> 00:23:50,950
+الـ Stationary phase قلنا إنه بتبدأ الخلايا هان
+
+313
+00:23:50,950 --> 00:23:53,730
+تتموت بنلاحظ أنه هان في عندي اللون الزهري بدل على
+
+314
+00:23:54,220 --> 00:23:57,620
+الـ death cells الخلايا اللي بتتموت بتبدأ الخلايا
+
+315
+00:23:57,620 --> 00:24:02,300
+انها تموت بكون عدد الخلايا اللي بتنمو بساوي عدد
+
+316
+00:24:02,300 --> 00:24:05,980
+الخلايا اللي بتموت في مرحلة الـ stationary phase
+
+317
+00:24:05,980 --> 00:24:10,940
+بعدين في مرحلة الـ death phase اللي هي بتبدأ اللي
+
+318
+00:24:10,940 --> 00:24:17,740
+هو عدد الخلايا اللي حية بيبدأ يقل بشكل لغرتمي هذا
+
+319
+00:24:17,740 --> 00:24:20,480
+بالنسبة لمراحل نمو البكتيريا
+
+320
+00:24:23,210 --> 00:24:26,930
+growth in continuous culture a continuous culture
+
+321
+00:24:26,930 --> 00:24:30,470
+is an open system اتفقنا إن ال continuous culture
+
+322
+00:24:30,470 --> 00:24:34,510
+هي عبارة عن نظام مفتوح على عكس ال batch culture
+
+323
+00:24:34,510 --> 00:24:37,730
+اللي كانت نظام مغلق in which fresh media is
+
+324
+00:24:37,730 --> 00:24:40,410
+continuously added to the culture at a constant
+
+325
+00:24:40,410 --> 00:24:44,450
+rate and all the broth is removed at the same rate
+
+326
+00:24:44,450 --> 00:24:48,870
+إيش قصده في هذه الجملة إنه بنفس الوقت اللي أنا بدي
+
+327
+00:24:48,870 --> 00:24:54,450
+أضيف فيه مغذياتنفس هذا الوقت هسحب الفضلات يبقى
+
+328
+00:24:54,450 --> 00:24:57,870
+المواد الغذائية اللى انا بدي اضيفها ل ال culture
+
+329
+00:24:57,870 --> 00:25:01,150
+لأن احنا اتفقنا ان ال continuous culture بضيف فيها
+
+330
+00:25:01,150 --> 00:25:05,190
+مواد غذائية بعد ال inculation ممكن اضيف مواد
+
+331
+00:25:05,190 --> 00:25:08,430
+غذائية لما اتخلص المواد الغذائية ممكن ان انا اضيف
+
+332
+00:25:08,430 --> 00:25:12,690
+مواد غذائية بعد ال inculation فبالتالي المواد
+
+333
+00:25:12,690 --> 00:25:17,230
+الغذائية اللى انا ضفتها هتكون بنفس الوقت اللى انا
+
+334
+00:25:17,230 --> 00:25:21,570
+بدي اسحب فيه الفضلات هيكون تنين بنفس ال rateكيف
+
+335
+00:25:21,570 --> 00:25:26,490
+بدي أسحب بقولي هذه العملية بستخدم جهاز اسمه الـ
+
+336
+00:25:26,490 --> 00:25:32,170
+Chemostatic هذا مهم هذا الجهاز اللي انا بدي اضيف
+
+337
+00:25:32,170 --> 00:25:35,510
+فيه اللي هو اللي بنفس الوقت اللي بدي اضيف فيه
+
+338
+00:25:35,510 --> 00:25:40,530
+مغاديات هسحب فضلات نفس الوقت بنفس الراتب نفس
+
+339
+00:25:40,530 --> 00:25:44,470
+المعدلالتركيز في الـ cell سيصل إلى مستوى
+
+340
+00:25:44,470 --> 00:25:48,290
+ايقاليبريام يبقى مستوى مستوى مستوى مستوى مستوى
+
+341
+00:25:48,290 --> 00:25:49,450
+مستوى مستوى مستوى مستوى مستوى مستوى مستوى مستوى
+
+342
+00:25:49,450 --> 00:25:50,070
+مستوى مستوى مستوى مستوى مستوى مستوى مستوى مستوى
+
+343
+00:25:50,070 --> 00:25:51,390
+مستوى مستوى مستوى مستوى مستوى مستوى مستوى مستوى
+
+344
+00:25:51,390 --> 00:25:52,150
+مستوى مستوى مستوى مستوى مستوى مستوى مستوى مستوى
+
+345
+00:25:52,150 --> 00:25:59,430
+مستوى مستوى مستوى مستوى مستوى مستوى مستوى مستوى
+
+346
+00:25:59,430 --> 00:26:08,810
+مستوى مستوى مستوى مستو
+
+347
+00:26:09,020 --> 00:26:14,820
+في وقت معين كل وقت معين بيتضاعف اللي هو العدد
+
+348
+00:26:14,820 --> 00:26:21,280
+الخلايا البكتيرية بمعدل ثابت ال
+
+349
+00:26:21,280 --> 00:26:25,360
+factor اللي بتأثر على النمو بشكل عام في ال slides
+
+350
+00:26:25,360 --> 00:26:29,720
+القبل كان في عوامل بتأثر على مثلا ال log phase على
+
+351
+00:26:29,720 --> 00:26:32,980
+ال lag phase على ال stationary phase لكن هنا
+
+352
+00:26:32,980 --> 00:26:36,880
+بنتكلم على النمو بشكل عام أول شئ درجة الحرارة أكيد
+
+353
+00:26:36,880 --> 00:26:40,630
+في بكتيريابتنمو على درجات حرارة عالية فى بكتيريا
+
+354
+00:26:40,630 --> 00:26:44,610
+بتنمو على درجات حرارة منخفضة معظم البكتيريا بتنمو
+
+355
+00:26:44,610 --> 00:26:49,370
+على درجات حرارة متوسطة ليها الـ 37 درجة سليسيا الـ
+
+356
+00:26:49,370 --> 00:26:54,550
+pH كمان الـ pH عندي طبعا بيختلف من بكتيريا
+
+357
+00:26:54,550 --> 00:26:58,070
+لبكتيريا فى بكتيريا بتنمو at alkaline pH على سبيل
+
+358
+00:26:58,070 --> 00:27:01,690
+الميثال الـ Vibrio cholera فى اللى هو الـ neutral
+
+359
+00:27:01,690 --> 00:27:06,930
+pH وفي الـ acidic pH فبيختلف من بكتيريا لأخرىتالي
+
+360
+00:27:06,930 --> 00:27:10,610
+تعامل الـ Salt Concentration أكيد في بكتيريا
+
+361
+00:27:10,610 --> 00:27:15,710
+بتتحمل الملوحة العالية بتتحمل 7.5% NACL زي ما
+
+362
+00:27:15,710 --> 00:27:21,390
+ذكرنا اللي هي عائلة الستافيلوكوكاس تمام؟ في بتتحمل
+
+363
+00:27:21,390 --> 00:27:26,550
+درجة 6.5% من اللي هو 6.5% NACL زي الـ Enterobacter
+
+364
+00:27:26,550 --> 00:27:32,310
+لكن باقي أنواع البكتيريا اللي هي بتتحمل 85% من 100
+
+365
+00:27:32,310 --> 00:27:36,710
+% NACLالاخر الشيء الـ Oxygen Concentration وهذا
+
+366
+00:27:36,710 --> 00:27:42,110
+شرحناه بـ Chapter كامل اتكلمنا عن كل التفاصيل اللي
+
+367
+00:27:42,110 --> 00:27:49,890
+هو في Chapter الـ Oxygen Requirement في
+
+368
+00:27:49,890 --> 00:27:53,490
+هاي الـ Slide هنتكلم كيف بدنا انقيز الـ Microbial
+
+369
+00:27:53,490 --> 00:27:57,970
+Growth كيف بدي اقيس مدى النمو راح اشوف عكارة هيكون
+
+370
+00:27:57,970 --> 00:28:03,000
+فيها دي Terbidity عكارةرح أقيصها من خلال جهاز
+
+371
+00:28:03,000 --> 00:28:07,440
+Spectrophotometer هقرأ الـ Absorbance طبعاً هيكون
+
+372
+00:28:07,440 --> 00:28:10,400
+في عندي Light Source هيمر الدوء عبر اللي هي الـ
+
+373
+00:28:10,400 --> 00:28:14,500
+Sample هيكون في عندي Turbidity عكارة هيصير في عندي
+
+374
+00:28:14,500 --> 00:28:19,260
+انتصاص اللي هو الـ Absorbance و بالنهاية هقرأ الـ
+
+375
+00:28:19,260 --> 00:28:24,580
+Absorbance لكل اللي هي في خلال كل وقت معني
+
+376
+00:28:28,270 --> 00:28:31,950
+بالنسبة للأدوات اللي هستخدمها في هذه التجربة أول
+
+377
+00:28:31,950 --> 00:28:35,630
+شي اللي هي لـ Tryptic-Cypros هاي ميديا سائلة هيكون
+
+378
+00:28:35,630 --> 00:28:40,690
+مزرعة فيها Non-pathogenic E.coli هتكون كـ Stock
+
+379
+00:28:40,690 --> 00:28:45,210
+culture عشان أخد منها انيكولام E.coli وأزرع في
+
+380
+00:28:45,210 --> 00:28:50,230
+ميديا أخرى انيكولات انجلوب عشان أزرع البكتيريا من
+
+381
+00:28:50,230 --> 00:28:54,950
+الـ Stock culture Spectrophotometer عشان أقرأ الـ
+
+382
+00:28:54,950 --> 00:28:59,040
+Absorbanceتست الـ tube راك بدي راك عشان أحط ال
+
+383
+00:28:59,040 --> 00:29:06,380
+tube فيه two tube of a tryptic soy broth واحدة
+
+384
+00:29:06,380 --> 00:29:11,980
+هتكون ل البلانك عشان أصفر عليها التانية عشان أزرع
+
+385
+00:29:11,980 --> 00:29:16,480
+فيها البكتيريا لال test هاخد من اللي هي ال stock
+
+386
+00:29:16,480 --> 00:29:21,200
+culture اللي موجود فيها E.coli و هزرع في tube أخرى
+
+387
+00:29:21,200 --> 00:29:24,020
+tryptic soy broth اللي هتكون مش وجود فيها
+
+388
+00:29:24,020 --> 00:29:27,890
+البكتيرياأكيد هيكون موجود في عندي أو بيلزمني
+
+389
+00:29:27,890 --> 00:29:32,150
+incubator عشان هنمي البكتيريا اللي عندي أنا
+
+390
+00:29:32,150 --> 00:29:35,570
+بالنهاية بدي أشوف الميكروبيا الإجراث معدل انمو
+
+391
+00:29:35,570 --> 00:29:41,050
+بنسيت بارنر بما أني هزرع بكتيريا فأكيد لازم أزرع
+
+392
+00:29:41,050 --> 00:29:46,250
+في ظروف sterile surface disinfectant هذا بالنسبة
+
+393
+00:29:46,250 --> 00:29:48,790
+للأدوات اللي هستخدمها في هذه التجربة
+
+394
+00:29:55,850 --> 00:29:59,530
+بالنسبة للخطوات أول شي هغسل أيدي ألبس ال gloves
+
+395
+00:29:59,530 --> 00:30:04,650
+أجهز أدواتي ومنطقة الشغل هشغل spectrophotometer و
+
+396
+00:30:04,650 --> 00:30:10,630
+أطبطه على 450 نانومتر هعمل warm up لجهاز لمدة 30
+
+397
+00:30:10,630 --> 00:30:14,570
+minute قبل ما أخد القراءة تاني شي هجيب ال two
+
+398
+00:30:14,570 --> 00:30:18,810
+tubes of tryptoxibros قلنا بدنا يكوننا two tubes
+
+399
+00:30:18,810 --> 00:30:23,920
+of tryptoxibros واحدة هتكون لل controlمش هزرع فيها
+
+400
+00:30:23,920 --> 00:30:28,560
+أي بكتيريا هتكون Uninculated فبالتالي هتكون
+
+401
+00:30:28,560 --> 00:30:32,220
+كcontrol شو هي الـ control؟ هي كل إشي بيكون فيها
+
+402
+00:30:32,220 --> 00:30:35,740
+معادة الشغل اللي أنا بدي أقرأها اللي أنا بدي أقرأه
+
+403
+00:30:35,740 --> 00:30:39,480
+هو الـ turbidity اللي جاي من البكتيريا و النموها
+
+404
+00:30:39,480 --> 00:30:44,080
+فبالتالي هان الـ control هتكون Media tryptoxide
+
+405
+00:30:44,080 --> 00:30:47,860
+broth لكن مش مزروع فيها بكتيريا فهتكون كcontrol
+
+406
+00:30:48,450 --> 00:30:51,710
+التيوب التاني هتكون اللي هي الـ sample اللي هزرع
+
+407
+00:30:51,710 --> 00:30:54,990
+فيها بكتيريا اللي هاخد الـ non-pathogenic E.coli
+
+408
+00:30:54,990 --> 00:30:59,010
+من الـ stock culture بقول لي حطي الـ control تيوب
+
+409
+00:30:59,010 --> 00:31:02,750
+في الـ sample holder of spectrophotometer شغلي
+
+410
+00:31:02,750 --> 00:31:07,320
+اللي هي الـ light control knob هتاخدي القراءةطبعا
+
+411
+00:31:07,320 --> 00:31:11,400
+القراءة هتكون إيش بالنسبة للـ Control أكيد هتكون
+
+412
+00:31:11,400 --> 00:31:16,260
+الامتصاص الـ Absorbance صفر لأن الـ Control Tube
+
+413
+00:31:16,260 --> 00:31:21,740
+مافيها أي بكتيريا هتكون Clear فبالتالي هيمر الضوء
+
+414
+00:31:21,740 --> 00:31:25,940
+احنا قولنا أنا بقرأ Terribility فبالتالي الـ
+
+415
+00:31:25,940 --> 00:31:30,060
+Control Tube هتكون عندي اللي هي Clear هيصير امتصاص
+
+416
+00:31:30,060 --> 00:31:33,140
+مش هيصير امتصاص لأنه مافي أي شيء هتكون الـ Tube
+
+417
+00:31:33,140 --> 00:31:38,180
+Clearفبالتالي الـ Absorbance هتكون 0 بالنسبة لـ
+
+418
+00:31:38,180 --> 00:31:43,040
+النافذية هتكون 100% ليش النافذية هتكون 100%؟ لأنه
+
+419
+00:31:43,040 --> 00:31:47,520
+الامتصاص سفر الضوء اللي دخل هو نفسه الضوء اللي طلع
+
+420
+00:31:47,520 --> 00:31:51,620
+فبالتالي النافذية 100% هشيل الـ Control الـ Tube
+
+421
+00:31:51,620 --> 00:31:57,460
+هيك بيكون صفرت على ال .. اللي هو صفرت الجهاز بيكون
+
+422
+00:31:57,460 --> 00:32:00,900
+الـ Spectrophotometer جاهز لقراة اللي هي الـ
+
+423
+00:32:00,900 --> 00:32:01,260
+Sample
+
+424
+00:32:05,500 --> 00:32:09,900
+بعدها بتشغلي اللى هو الـ Benson burner بتعملي اللى
+
+425
+00:32:09,900 --> 00:32:15,160
+هو sterilization للـ loop هتخليها تبرد شوية بعدها
+
+426
+00:32:15,160 --> 00:32:20,200
+هتاخدى Ecolime من ال stock culture هتزراعيها في ال
+
+427
+00:32:20,200 --> 00:32:26,880
+acid tube اللى قولنا للـ sample بعدها طبعا هتاخدى
+
+428
+00:32:26,880 --> 00:32:31,920
+اللى هتزراعيها في ال tube طبعا هتعملي تعقيم لل
+
+429
+00:32:31,920 --> 00:32:37,400
+loop بعد استخدامتههتبدأ تقرأ الـ Turbidity لـ S
+
+430
+00:32:37,400 --> 00:32:41,120
+-Tube في الـ Sample Well لـ Spectrophotometer
+
+431
+00:32:41,120 --> 00:32:45,880
+هتاخد قراءة الـ Absorbance تمام؟ بعد ما أخدت قراءة
+
+432
+00:32:45,880 --> 00:32:49,480
+الـ Absorbance هتاخد الـ Tube وتحطيها ترجعيها في
+
+433
+00:32:49,480 --> 00:32:54,520
+الـ Incubator خلال مدة معينة مثلا ممكن أخد القراءة
+
+434
+00:32:54,520 --> 00:32:59,920
+خلال مثلا مكتوب في جدول ممكن ناخد كل مثلا خمس
+
+435
+00:32:59,920 --> 00:33:04,520
+دقايق أخد قراءةأحط أخد قراءة وبعدين أحط اللي هي
+
+436
+00:33:04,520 --> 00:33:08,200
+الـ Sample في الـ Incubator خمس دقائق وبعدين أخد
+
+437
+00:33:08,200 --> 00:33:12,880
+القراءة مرتين هيكون في عندي جدول في الـ Time وجدول
+
+438
+00:33:12,880 --> 00:33:17,040
+فيه مقابله هيكون اللي هي القراءة في كل وقت الـ
+
+439
+00:33:17,040 --> 00:33:20,440
+Absorbance بعد ما أخلص طبعا هسكر جهاز الـ
+
+440
+00:33:20,440 --> 00:33:25,700
+Spectrophotometer هجهز ورقة أرسم اللي هو محور لـ
+
+441
+00:33:25,700 --> 00:33:29,400
+Absorbance اللي هي القراءات اللي أنا أخدتها مع الـ
+
+442
+00:33:29,400 --> 00:33:35,630
+Time الوقت في كلطبعا احنا قلنا بناخد كل خمس دقايق
+
+443
+00:33:35,630 --> 00:33:40,910
+فمثلا أول شئ كانت القراءة على سبيل المثال Zero
+
+444
+00:33:40,910 --> 00:33:45,310
+بعدين بعد خمس دقايق كانت القراءة ال absorbance
+
+445
+00:33:45,310 --> 00:33:49,310
+اتنين بعد خمس دقايق طبعا عند عشر دقايق يكون وقتها
+
+446
+00:33:49,310 --> 00:33:54,150
+المدة كانت تلاتة وهكذا هاخد طبعا قراءات هعمل اللي
+
+447
+00:33:54,150 --> 00:33:59,020
+هو محاور لال absorbance و ال timeبعد ما اخلص طبعا
+
+448
+00:33:59,020 --> 00:34:07,240
+هنظف المكان و هشلح لي gloves و اغسل ايدايا هبدأ
+
+449
+00:34:07,240 --> 00:34:12,120
+اسجل اللي هو ال absorbance لكل وقت معين حسب الجدول
+
+450
+00:34:12,120 --> 00:34:16,110
+مناللاحظ انهعند كل وقت معين هيكون فيه قيمة
+
+451
+00:34:16,110 --> 00:34:21,110
+Absorbance مختلفة هعمل بالنهاية XY Axis هيكون لأ
+
+452
+00:34:21,110 --> 00:34:24,630
+لـ Absorbance و لـ Time هعمل الـ Chart بالنهاية
+
+453
+00:34:24,630 --> 00:34:28,550
+هيوضح اللي هو الـ Growth Curve الـ Lag Phase الـ X
+
+454
+00:34:28,550 --> 00:34:32,930
+Potential Phase والستشانري فازليش متذكر الـ Death
+
+455
+00:34:32,930 --> 00:34:35,610
+Face؟ لأنه إحنا قولنا في الـ Terpidometric
+
+456
+00:34:35,610 --> 00:34:40,770
+Measurement ما بقدر ألاحظ الـ Death Face لأن الـ
+
+457
+00:34:40,770 --> 00:34:44,450
+Count بيكون لـ الـ Death Cell و الـ Viable Cell مش
+
+458
+00:34:44,450 --> 00:34:47,770
+هقدر أميزهم عن بعض في الـ Microscopic Count و في
+
+459
+00:34:47,770 --> 00:34:51,370
+الـ Terpidometric Measurement لكن في الـ Black
+
+460
+00:34:51,370 --> 00:34:53,170
+Count بقدر أميز
+
+461
+00:34:58,830 --> 00:35:04,370
+هذا الـ curve موضح المراحل النمو فيه أو نستاج فيه
+
+462
+00:35:04,370 --> 00:35:10,650
+صورة يبدو أن هذا صراحة أفضل من ال curve اللي مار
+
+463
+00:35:10,650 --> 00:35:15,150
+معناه أول شيء في ال lag phase مافي عندي خلايا في
+
+464
+00:35:15,150 --> 00:35:19,030
+ال log phase اللي هو في عندي عدد الخلايا الحية
+
+465
+00:35:19,030 --> 00:35:22,970
+أكتر من عدد الخلايا الميتة إذا مانلاحظ تحت مكتوب
+
+466
+00:35:22,970 --> 00:35:27,030
+أن ال death cell بكون لونها أسود ال viable cellفي
+
+467
+00:35:27,030 --> 00:35:31,210
+ND زي نقاط كتيرة لكن في أول مرحلة بيكون في ND few
+
+468
+00:35:31,210 --> 00:35:34,550
+cells اللي هي النقاط القليلة في ال look phase
+
+469
+00:35:34,550 --> 00:35:40,710
+منلاحظ أن ال viable cell أكتر من ال death cell في
+
+470
+00:35:40,710 --> 00:35:44,290
+ال stationary phase عدد الخلايا الميتة بيساوي عدد
+
+471
+00:35:44,290 --> 00:35:48,190
+الخلايا الحية في ال death phase منلاحظ أن عدد
+
+472
+00:35:48,190 --> 00:35:54,140
+الخلايا الميتة أكتر من عدد الخلايا الحيةطبعا هذا
+
+473
+00:35:54,140 --> 00:35:57,960
+ال curve هو المظبوط أكتر من ال curve اللي مار
+
+474
+00:35:57,960 --> 00:36:03,240
+معناه لأنه موضح كل المراحل أو انبين عدد الخلايا
+
+475
+00:36:03,240 --> 00:36:04,200
+بشكل أفضل
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/PYxwZLDpRfI_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/PYxwZLDpRfI_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..2467213b748646399c26a05650e5328b7d5bcd6d
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/PYxwZLDpRfI_raw.json
@@ -0,0 +1 @@
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نفسها من الأضرار واصضاماتها ده كله بيأثر على اللي هو الطول ال lag fest تالت factor time required for synthesis of essential coenzyme or division factor", "tokens": [6027, 30543, 2655, 13672, 1829, 39894, 33753, 26108, 3224, 29538, 29739, 4117, 2655, 13546, 25528, 6225, 8592, 7649, 6055, 2655, 4117, 33911, 20449, 2423, 41779, 9566, 13672, 1829, 41850, 4032, 11242, 34268, 3224, 8978, 11296, 20449, 2423, 3021, 13672, 1829, 41850, 4032, 11242, 34268, 11296, 8978, 11296, 34051, 6055, 3615, 6027, 7435, 8717, 36178, 11296, 9154, 16247, 11242, 2288, 9640, 4032, 33546, 11242, 10943, 9307, 11296, 11778, 3224, 28242, 3224, 4724, 1829, 10721, 49115, 15844, 13672, 1829, 31439, 41950, 12610, 2423, 8953, 6633, 6055, 6027, 2655, 5952, 565, 4739, 337, 30252, 295, 7115, 598, 268, 1229, 1398, 420, 10044, 5952], "avg_logprob": -0.15965346062537467, "compression_ratio": 1.632, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 805.16, "end": 805.78, "word": "الوقت", "probability": 0.8798828125}, 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المثال لها تتضعف البكتيريا الواحدة وتصير اتناء طبعا هذا بسميه اللي هو الزيادة اللغارتمية or الأسية growing by geometric progression هذا بالنسبة لـ Geometric progression هو الزيادة الأسية واللغارتمية أن البكتيريا بتتضعف بمعدل ثابت كل خمس دقايق بتتضعف", "tokens": [40228, 22653, 28242, 16490, 2304, 3794, 11778, 4587, 47302, 4587, 15844, 8608, 21292, 1211, 9673, 12984, 6027, 5296, 11296, 6055, 2655, 11242, 3615, 5172, 29739, 4117, 2655, 13546, 25528, 2423, 14407, 24401, 3660, 34683, 9381, 13546, 1975, 2655, 1863, 16606, 23032, 3555, 3615, 995, 23758, 4724, 38251, 1829, 3224, 13672, 1829, 31439, 6024, 110, 1829, 18513, 3660, 13672, 17082, 9640, 39237, 10632, 420, 16247, 3794, 10632, 4194, 538, 33246, 18733, 23758, 20666, 1863, 35457, 3660, 5296, 39184, 2876, 29470, 18733, 31439, 6024, 110, 1829, 18513, 3660, 16247, 3794, 10632, 16070, 1211, 17082, 9640, 39237, 10632, 14739, 29739, 4117, 2655, 13546, 25528, 39894, 2655, 11242, 3615, 5172, 4724, 2304, 22488, 1211, 38637, 16758, 2655, 28242, 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"word": " بيتمد", "probability": 0.8309326171875}, {"start": 1229.63, "end": 1229.79, "word": " على", "probability": 0.82861328125}, {"start": 1229.79, "end": 1230.15, "word": " هاي", "probability": 0.85595703125}, {"start": 1230.15, "end": 1231.03, "word": " العوامل", "probability": 0.9847412109375}, {"start": 1231.03, "end": 1231.83, "word": " بالنسبة", "probability": 0.9827880859375}, {"start": 1231.83, "end": 1232.65, "word": " لرابع", "probability": 0.93115234375}, {"start": 1232.65, "end": 1233.23, "word": " مرحلة", "probability": 0.992919921875}, {"start": 1233.23, "end": 1233.43, "word": " اللي", "probability": 0.94384765625}, {"start": 1233.43, "end": 1233.77, "word": " هي", "probability": 0.92626953125}, {"start": 1233.77, "end": 1234.07, "word": " ال", "probability": 0.92626953125}, {"start": 1234.07, "end": 1234.35, "word": " death", "probability": 0.53125}, {"start": 1234.35, "end": 1235.01, "word": " phase", "probability": 0.875}, {"start": 1235.01, "end": 1235.59, "word": " if", "probability": 0.8671875}, {"start": 1235.59, "end": 1236.33, "word": " incubation", "probability": 0.953369140625}, {"start": 1236.33, "end": 1236.95, "word": " continues", "probability": 0.464599609375}, {"start": 1236.95, "end": 1237.59, "word": " after", "probability": 0.9072265625}, {"start": 1237.59, "end": 1237.85, "word": " the", "probability": 0.77978515625}, {"start": 1237.85, "end": 1238.45, "word": " population", "probability": 0.96044921875}, {"start": 1238.45, "end": 1239.53, "word": " reaches", "probability": 0.43017578125}, {"start": 1239.53, "end": 1240.15, "word": " stationary", "probability": 0.73388671875}, {"start": 1240.15, "end": 1240.51, "word": " phase", "probability": 0.97802734375}, {"start": 1240.51, "end": 1240.89, "word": " قولي", "probability": 0.7581380208333334}, {"start": 1240.89, "end": 1241.11, "word": " لو", "probability": 0.98486328125}], "temperature": 1.0}, {"id": 47, "seek": 127033, "start": 1241.34, "end": 1270.34, "text": "الـ Incubation ظل مستمر بعد ما أوصل لمرحلة الـ stationary phase اللي هو معدل إنه بيكون فيها ثابت a death phase follows بعدها بتبدأ مرحلة الـ death phase in which the viable cell population decline بقوللي إنه عدد الخلايا اللي بتنمو في الـ death phase ها بتبدأ اتقل في مرحلة الـ stationary قولنا إن الـ viable cell بتساوي اللي هي الخلايا الحية بتساوي الخلايا الميتة", "tokens": [6027, 39184, 7779, 836, 399, 1357, 116, 1211, 3714, 14851, 29973, 39182, 19446, 34051, 36520, 5296, 29973, 5016, 37977, 2423, 39184, 30452, 5574, 13672, 1829, 31439, 20449, 3215, 1211, 36145, 3224, 4724, 1829, 30544, 8978, 11296, 38637, 16758, 2655, 257, 2966, 5574, 10002, 39182, 11296, 39894, 44510, 10721, 3714, 2288, 5016, 37977, 2423, 39184, 2966, 5574, 294, 597, 264, 22024, 2815, 4415, 15635, 4724, 39648, 20292, 36145, 3224, 6225, 3215, 3215, 33962, 15040, 25528, 13672, 1829, 39894, 1863, 2304, 2407, 8978, 2423, 39184, 2966, 5574, 8032, 995, 39894, 44510, 10721, 1975, 2655, 4587, 1211, 8978, 3714, 2288, 5016, 37977, 2423, 39184, 30452, 12174, 12610, 8315, 36145, 2423, 39184, 22024, 2815, 39894, 3794, 995, 45865, 13672, 1829, 39896, 33962, 15040, 25528, 21542, 10632, 39894, 3794, 995, 45865, 33962, 15040, 25528, 9673, 36081, 3660], "avg_logprob": -0.18585525598741115, "compression_ratio": 2.0148698884758365, "no_speech_prob": 6.765127182006836e-05, "words": [{"start": 1241.34, "end": 1241.62, "word": "الـ", "probability": 0.8876953125}, {"start": 1241.62, "end": 1242.14, "word": " Incubation", "probability": 0.9020182291666666}, {"start": 1242.14, "end": 1242.38, "word": " ظل", "probability": 0.64300537109375}, {"start": 1242.38, "end": 1242.9, "word": " مستمر", "probability": 0.8564453125}, {"start": 1242.9, "end": 1243.18, "word": " بعد", "probability": 0.95947265625}, {"start": 1243.18, "end": 1243.28, "word": " ما", "probability": 0.81005859375}, {"start": 1243.28, "end": 1243.64, "word": " أوصل", "probability": 0.8623046875}, {"start": 1243.64, "end": 1244.2, "word": " لمرحلة", "probability": 0.885498046875}, {"start": 1244.2, "end": 1244.42, "word": " الـ", "probability": 0.6644287109375}, {"start": 1244.42, "end": 1244.86, "word": " stationary", "probability": 0.62646484375}, {"start": 1244.86, "end": 1245.34, "word": " phase", "probability": 0.93408203125}, {"start": 1245.34, "end": 1245.54, "word": " اللي", "probability": 0.85107421875}, {"start": 1245.54, "end": 1245.7, "word": " هو", "probability": 0.96875}, {"start": 1245.7, "end": 1246.12, "word": " معدل", "probability": 0.8938802083333334}, {"start": 1246.12, "end": 1246.44, "word": " إنه", "probability": 0.577392578125}, {"start": 1246.44, "end": 1246.82, "word": " بيكون", "probability": 0.7784830729166666}, {"start": 1246.82, "end": 1247.16, "word": " فيها", "probability": 0.96142578125}, {"start": 1247.16, "end": 1247.76, "word": " ثابت", "probability": 0.99658203125}, {"start": 1247.76, "end": 1248.54, "word": " a", "probability": 0.28173828125}, {"start": 1248.54, "end": 1248.86, "word": " death", "probability": 0.94873046875}, {"start": 1248.86, "end": 1249.3, "word": " phase", "probability": 0.93310546875}, {"start": 1249.3, "end": 1249.76, "word": " follows", "probability": 0.68994140625}, {"start": 1249.76, "end": 1250.6, "word": " بعدها", "probability": 0.921142578125}, {"start": 1250.6, "end": 1251.2, "word": " بتبدأ", "probability": 0.89794921875}, {"start": 1251.2, "end": 1251.78, "word": " مرحلة", "probability": 0.9913330078125}, {"start": 1251.78, "end": 1252.06, "word": " الـ", "probability": 0.904541015625}, {"start": 1252.06, "end": 1252.3, "word": " death", "probability": 0.7958984375}, {"start": 1252.3, "end": 1252.94, "word": " phase", "probability": 0.958984375}, {"start": 1252.94, "end": 1253.96, "word": " in", "probability": 0.78759765625}, {"start": 1253.96, "end": 1254.42, "word": " which", "probability": 0.97314453125}, {"start": 1254.42, "end": 1255.04, "word": " the", "probability": 0.82470703125}, {"start": 1255.04, "end": 1255.9, "word": " viable", "probability": 0.86376953125}, {"start": 1255.9, "end": 1256.26, "word": " cell", "probability": 0.94873046875}, {"start": 1256.26, "end": 1256.84, "word": " population", "probability": 0.94140625}, {"start": 1256.84, "end": 1257.46, "word": " decline", "probability": 0.37646484375}, {"start": 1257.46, "end": 1258.04, "word": " بقوللي", "probability": 0.7120768229166666}, {"start": 1258.04, "end": 1258.46, "word": " إنه", "probability": 0.567138671875}, {"start": 1258.46, "end": 1258.96, "word": " عدد", "probability": 0.88427734375}, {"start": 1258.96, "end": 1259.7, "word": " الخلايا", "probability": 0.9401041666666666}, {"start": 1259.7, "end": 1259.96, "word": " اللي", "probability": 0.95751953125}, {"start": 1259.96, "end": 1260.42, "word": " بتنمو", "probability": 0.9029541015625}, {"start": 1260.42, "end": 1260.54, "word": " في", "probability": 0.9423828125}, {"start": 1260.54, "end": 1260.72, "word": " الـ", "probability": 0.717529296875}, {"start": 1260.72, "end": 1260.94, "word": " death", "probability": 0.921875}, {"start": 1260.94, "end": 1261.26, "word": " phase", "probability": 0.9697265625}, {"start": 1261.26, "end": 1261.62, "word": " ها", "probability": 0.4273681640625}, {"start": 1261.62, "end": 1262.26, "word": " بتبدأ", "probability": 0.9415690104166666}, {"start": 1262.26, "end": 1262.82, "word": " اتقل", "probability": 0.8875732421875}, {"start": 1262.82, "end": 1263.4, "word": " في", "probability": 0.927734375}, {"start": 1263.4, "end": 1263.88, "word": " مرحلة", "probability": 0.9947509765625}, {"start": 1263.88, "end": 1264.12, "word": " الـ", "probability": 0.757568359375}, {"start": 1264.12, "end": 1264.46, "word": " stationary", "probability": 0.970703125}, {"start": 1264.46, "end": 1265.1, "word": " قولنا", "probability": 0.8404947916666666}, {"start": 1265.1, "end": 1265.3, "word": " إن", "probability": 0.7255859375}, {"start": 1265.3, "end": 1265.52, "word": " الـ", "probability": 0.6806640625}, {"start": 1265.52, "end": 1265.76, "word": " viable", "probability": 0.88623046875}, {"start": 1265.76, "end": 1266.16, "word": " cell", "probability": 0.94091796875}, {"start": 1266.16, "end": 1266.94, "word": " بتساوي", "probability": 0.8719482421875}, {"start": 1266.94, "end": 1268.12, "word": " اللي", "probability": 0.969970703125}, {"start": 1268.12, "end": 1268.3, "word": " هي", "probability": 0.970703125}, {"start": 1268.3, "end": 1268.7, "word": " الخلايا", "probability": 0.978515625}, {"start": 1268.7, "end": 1268.96, "word": " الحية", "probability": 0.83349609375}, {"start": 1268.96, "end": 1269.38, "word": " بتساوي", "probability": 0.9635009765625}, {"start": 1269.38, "end": 1269.82, "word": " الخلايا", "probability": 0.9905598958333334}, {"start": 1269.82, "end": 1270.34, "word": " الميتة", "probability": 0.9537760416666666}], "temperature": 1.0}, {"id": 48, "seek": 129933, "start": 1270.7, "end": 1299.34, "text": "لكن في الـ death face الـ viable cells الخلايا الحية بتبدأ اتقل هندقلي ملاحظة أنا قولتها في chapter الـ Serial Dilution if counting by terpidometric measurement اللي هو الـ spectrophotometer or microscopic count the death face cannot be observed قولنا إنه أنا ما بميز الخلايا الحية والخلايا الميتة لو أنا عديت في ال terpidometric measurement", "tokens": [1211, 19452, 8978, 2423, 39184, 2966, 1851, 2423, 39184, 22024, 5438, 33962, 15040, 25528, 21542, 10632, 39894, 44510, 10721, 1975, 2655, 4587, 1211, 8032, 41260, 4587, 20292, 3714, 15040, 5016, 19913, 3660, 41850, 12174, 12610, 2655, 11296, 8978, 7187, 2423, 39184, 4210, 831, 36475, 1448, 498, 13251, 538, 1796, 79, 327, 29470, 13160, 13672, 1829, 31439, 2423, 39184, 6177, 11741, 310, 13606, 420, 47897, 1207, 264, 2966, 1851, 2644, 312, 13095, 12174, 12610, 8315, 36145, 3224, 41850, 19446, 4724, 2304, 1829, 11622, 33962, 15040, 25528, 21542, 10632, 16070, 9778, 15040, 25528, 9673, 36081, 3660, 45164, 41850, 6225, 16254, 2655, 8978, 2423, 1796, 79, 327, 29470, 13160], "avg_logprob": -0.21144860035905214, "compression_ratio": 1.7378277153558053, "no_speech_prob": 6.556510925292969e-07, "words": [{"start": 1270.7, "end": 1271.18, "word": "لكن", "probability": 0.90771484375}, {"start": 1271.18, "end": 1271.44, "word": " في", "probability": 0.98095703125}, {"start": 1271.44, "end": 1271.74, "word": " الـ", "probability": 0.970458984375}, {"start": 1271.74, "end": 1272.0, "word": " death", "probability": 0.0830078125}, {"start": 1272.0, "end": 1272.32, "word": " face", "probability": 0.426513671875}, {"start": 1272.32, "end": 1272.76, "word": " الـ", "probability": 0.71484375}, {"start": 1272.76, "end": 1273.12, "word": " viable", "probability": 0.75390625}, {"start": 1273.12, "end": 1273.82, "word": " cells", "probability": 0.91943359375}, {"start": 1273.82, "end": 1274.46, "word": " الخلايا", "probability": 0.9347330729166666}, {"start": 1274.46, "end": 1274.86, "word": " الحية", "probability": 0.889404296875}, {"start": 1274.86, "end": 1275.48, "word": " بتبدأ", "probability": 0.90576171875}, {"start": 1275.48, "end": 1276.18, "word": " اتقل", "probability": 0.8636474609375}, {"start": 1276.18, "end": 1277.24, "word": " هندقلي", "probability": 0.55010986328125}, {"start": 1277.24, "end": 1277.96, "word": " ملاحظة", "probability": 0.98935546875}, {"start": 1277.96, "end": 1278.14, "word": " أنا", "probability": 0.67041015625}, {"start": 1278.14, "end": 1278.52, "word": " قولتها", "probability": 0.86248779296875}, {"start": 1278.52, "end": 1278.84, "word": " في", "probability": 0.97998046875}, {"start": 1278.84, "end": 1279.56, "word": " chapter", "probability": 0.5458984375}, {"start": 1279.56, "end": 1279.96, "word": " الـ", "probability": 0.7232666015625}, {"start": 1279.96, "end": 1280.32, "word": " Serial", "probability": 0.8583984375}, {"start": 1280.32, "end": 1280.84, "word": " Dilution", "probability": 0.75390625}, {"start": 1280.84, "end": 1281.62, "word": " if", "probability": 0.6201171875}, {"start": 1281.62, "end": 1282.28, "word": " counting", "probability": 0.94091796875}, {"start": 1282.28, "end": 1282.88, "word": " by", "probability": 0.97998046875}, {"start": 1282.88, "end": 1284.04, "word": " terpidometric", "probability": 0.5859375}, {"start": 1284.04, "end": 1284.9, "word": " measurement", "probability": 0.82275390625}, {"start": 1284.9, "end": 1285.14, "word": " اللي", "probability": 0.9580078125}, {"start": 1285.14, "end": 1285.34, "word": " هو", "probability": 0.99365234375}, {"start": 1285.34, "end": 1285.58, "word": " الـ", "probability": 0.87451171875}, {"start": 1285.58, "end": 1287.2, "word": " spectrophotometer", "probability": 0.8602294921875}, {"start": 1287.2, "end": 1288.04, "word": " or", "probability": 0.8212890625}, {"start": 1288.04, "end": 1288.74, "word": " microscopic", "probability": 0.85498046875}, {"start": 1288.74, "end": 1289.62, "word": " count", "probability": 0.908203125}, {"start": 1289.62, "end": 1290.24, "word": " the", "probability": 0.5751953125}, {"start": 1290.24, "end": 1290.56, "word": " death", "probability": 0.96630859375}, {"start": 1290.56, "end": 1291.02, "word": " face", "probability": 0.88427734375}, {"start": 1291.02, "end": 1291.28, "word": " cannot", "probability": 0.8251953125}, {"start": 1291.28, "end": 1291.68, "word": " be", "probability": 0.96533203125}, {"start": 1291.68, "end": 1292.22, "word": " observed", "probability": 0.85595703125}, {"start": 1292.22, "end": 1292.94, "word": " قولنا", "probability": 0.9064127604166666}, {"start": 1292.94, "end": 1293.28, "word": " إنه", "probability": 0.6328125}, {"start": 1293.28, "end": 1293.54, "word": " أنا", "probability": 0.88427734375}, {"start": 1293.54, "end": 1294.12, "word": " ما", "probability": 0.84619140625}, {"start": 1294.12, "end": 1294.6, "word": " بميز", "probability": 0.9720458984375}, {"start": 1294.6, "end": 1295.12, "word": " الخلايا", "probability": 0.9749348958333334}, {"start": 1295.12, "end": 1295.46, "word": " الحية", "probability": 0.984619140625}, {"start": 1295.46, "end": 1296.04, "word": " والخلايا", "probability": 0.881591796875}, {"start": 1296.04, "end": 1296.64, "word": " الميتة", "probability": 0.9111328125}, {"start": 1296.64, "end": 1296.78, "word": " لو", "probability": 0.9931640625}, {"start": 1296.78, "end": 1297.0, "word": " أنا", "probability": 0.8896484375}, {"start": 1297.0, "end": 1297.4, "word": " عديت", "probability": 0.8523763020833334}, {"start": 1297.4, "end": 1297.54, "word": " في", "probability": 0.97216796875}, {"start": 1297.54, "end": 1297.78, "word": " ال", "probability": 0.93896484375}, {"start": 1297.78, "end": 1298.56, "word": " terpidometric", "probability": 0.80792236328125}, {"start": 1298.56, "end": 1299.34, "word": " measurement", "probability": 0.87109375}], "temperature": 1.0}, {"id": 49, "seek": 132734, "start": 1300.18, "end": 1327.34, "text": "أو لو استخدمت الـ Microscopic Count لو استخدمت الـ Microscope ما بفرق بين الخلايا الحية والخلايا الميتة فقط في طريقة اللي هي الـ Viable Cells اللي هي الـ Blood Count هي اللي بميز الخلايا الحية والميتة هي اللي بتعد فقط الـ Viable Cell ما بتعد الخلايا الميتة لأنه الأكيد البكتيريا اللي ماتت مش هتنمو على الطبق", "tokens": [10721, 2407, 45164, 44713, 9778, 40448, 2655, 2423, 39184, 5818, 38006, 299, 5247, 45164, 44713, 9778, 40448, 2655, 2423, 39184, 5818, 2635, 13960, 19446, 4724, 5172, 2288, 4587, 49374, 33962, 15040, 25528, 21542, 10632, 16070, 9778, 15040, 25528, 9673, 36081, 3660, 6156, 47432, 8978, 23032, 16572, 28671, 13672, 1829, 39896, 2423, 39184, 6626, 712, 383, 13677, 13672, 1829, 39896, 2423, 39184, 17428, 5247, 39896, 13672, 1829, 4724, 2304, 1829, 11622, 33962, 15040, 25528, 21542, 10632, 16070, 2304, 36081, 3660, 39896, 13672, 1829, 39894, 22488, 6156, 47432, 2423, 39184, 6626, 712, 28859, 19446, 39894, 22488, 33962, 15040, 25528, 9673, 36081, 3660, 5296, 33456, 3224, 16247, 4117, 25708, 29739, 4117, 2655, 13546, 25528, 13672, 1829, 3714, 9307, 2655, 37893, 8032, 2655, 1863, 2304, 2407, 15844, 41950, 3555, 4587], "avg_logprob": -0.09824065183560679, 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"end": 2152.63, "text": "في ND زي نقاط كتيرة لكن في أول مرحلة بيكون في ND few cells اللي هي النقاط القليلة في ال look phase منلاحظ أن ال viable cell أكتر من ال death cell في ال stationary phase عدد الخلايا الميتة بيساوي عدد الخلايا الحية في ال death phase منلاحظ أن عدد الخلايا الميتة أكتر من عدد الخلايا الحية", "tokens": [41185, 40709, 30767, 1829, 8717, 4587, 41193, 9122, 2655, 48923, 44381, 8978, 5551, 12610, 3714, 2288, 5016, 37977, 4724, 1829, 30544, 8978, 40709, 1326, 5438, 13672, 1829, 39896, 28239, 4587, 41193, 25062, 20292, 37977, 8978, 2423, 574, 5574, 9154, 15040, 5016, 19913, 14739, 2423, 22024, 2815, 5551, 4117, 2655, 2288, 9154, 2423, 2966, 2815, 8978, 2423, 30452, 5574, 6225, 3215, 3215, 33962, 15040, 25528, 9673, 36081, 3660, 4724, 1829, 3794, 995, 45865, 6225, 3215, 3215, 33962, 15040, 25528, 21542, 10632, 8978, 2423, 2966, 5574, 9154, 15040, 5016, 19913, 14739, 6225, 3215, 3215, 33962, 15040, 25528, 9673, 36081, 3660, 5551, 4117, 2655, 2288, 9154, 6225, 3215, 3215, 33962, 15040, 25528, 21542, 10632], "avg_logprob": -0.14355468869741475, "compression_ratio": 2.1179245283018866, "no_speech_prob": 3.7550926208496094e-06, "words": [{"start": 2126.79, "end": 2127.03, "word": "في", "probability": 0.483642578125}, {"start": 2127.03, "end": 2127.35, "word": " ND", "probability": 0.40087890625}, {"start": 2127.35, "end": 2127.93, "word": " زي", "probability": 0.869873046875}, {"start": 2127.93, "end": 2128.61, "word": " نقاط", "probability": 0.8958333333333334}, {"start": 2128.61, "end": 2129.03, "word": " كتيرة", "probability": 0.8577473958333334}, {"start": 2129.03, "end": 2129.43, "word": " لكن", "probability": 0.83154296875}, {"start": 2129.43, "end": 2129.69, "word": " في", "probability": 0.923828125}, {"start": 2129.69, "end": 2129.95, "word": " أول", "probability": 0.952880859375}, {"start": 2129.95, "end": 2130.37, "word": " مرحلة", "probability": 0.991943359375}, {"start": 2130.37, "end": 2130.65, "word": " بيكون", "probability": 0.8948567708333334}, {"start": 2130.65, "end": 2130.79, "word": " في", "probability": 0.900390625}, {"start": 2130.79, "end": 2130.95, "word": " ND", "probability": 0.63427734375}, {"start": 2130.95, "end": 2131.21, "word": " few", "probability": 0.3662109375}, {"start": 2131.21, "end": 2131.55, "word": " cells", "probability": 0.8583984375}, {"start": 2131.55, "end": 2131.69, "word": " اللي", "probability": 0.932861328125}, {"start": 2131.69, "end": 2131.85, "word": " هي", "probability": 0.95947265625}, {"start": 2131.85, "end": 2132.67, "word": " النقاط", "probability": 0.9207356770833334}, {"start": 2132.67, "end": 2133.19, "word": " القليلة", "probability": 0.8297526041666666}, {"start": 2133.19, "end": 2133.83, "word": " في", "probability": 0.94287109375}, {"start": 2133.83, "end": 2134.03, "word": " ال", "probability": 0.8388671875}, {"start": 2134.03, "end": 2134.21, "word": " look", "probability": 0.10931396484375}, {"start": 2134.21, "end": 2134.55, "word": " phase", "probability": 0.76953125}, {"start": 2134.55, "end": 2135.17, "word": " منلاحظ", "probability": 0.771209716796875}, {"start": 2135.17, "end": 2135.41, "word": " أن", "probability": 0.6416015625}, {"start": 2135.41, "end": 2136.27, "word": " ال", "probability": 0.5224609375}, {"start": 2136.27, "end": 2136.79, "word": " viable", "probability": 0.85009765625}, {"start": 2136.79, "end": 2137.19, "word": " cell", "probability": 0.94091796875}, {"start": 2137.19, "end": 2137.79, "word": " أكتر", "probability": 0.9769287109375}, {"start": 2137.79, "end": 2138.11, "word": " من", "probability": 0.99560546875}, {"start": 2138.11, "end": 2138.53, "word": " ال", "probability": 0.98486328125}, {"start": 2138.53, "end": 2138.95, "word": " death", "probability": 0.6953125}, {"start": 2138.95, "end": 2140.15, "word": " cell", "probability": 0.9501953125}, {"start": 2140.15, "end": 2140.71, "word": " في", "probability": 0.76611328125}, {"start": 2140.71, "end": 2140.91, "word": " ال", "probability": 0.95166015625}, {"start": 2140.91, "end": 2141.41, "word": " stationary", "probability": 0.69775390625}, {"start": 2141.41, "end": 2142.07, "word": " phase", "probability": 0.9423828125}, {"start": 2142.07, "end": 2142.71, "word": " عدد", "probability": 0.9763997395833334}, {"start": 2142.71, "end": 2143.15, "word": " الخلايا", "probability": 0.9500325520833334}, {"start": 2143.15, "end": 2143.63, "word": " الميتة", "probability": 0.93798828125}, {"start": 2143.63, "end": 2143.97, "word": " بيساوي", "probability": 0.753125}, {"start": 2143.97, "end": 2144.29, "word": " عدد", "probability": 0.9967447916666666}, {"start": 2144.29, "end": 2144.85, "word": " الخلايا", "probability": 0.98291015625}, {"start": 2144.85, "end": 2145.45, "word": " الحية", "probability": 0.951171875}, {"start": 2145.45, "end": 2146.11, "word": " في", "probability": 0.9716796875}, {"start": 2146.11, "end": 2146.45, "word": " ال", "probability": 0.9765625}, {"start": 2146.45, "end": 2146.71, "word": " death", "probability": 0.96923828125}, {"start": 2146.71, "end": 2147.05, "word": " phase", "probability": 0.93359375}, {"start": 2147.05, "end": 2147.61, "word": " منلاحظ", "probability": 0.97412109375}, {"start": 2147.61, "end": 2147.81, "word": " أن", "probability": 0.93212890625}, {"start": 2147.81, "end": 2148.19, "word": " عدد", "probability": 0.9934895833333334}, {"start": 2148.19, "end": 2148.81, "word": " الخلايا", "probability": 0.9830729166666666}, {"start": 2148.81, "end": 2149.99, "word": " الميتة", "probability": 0.9833984375}, {"start": 2149.99, "end": 2150.79, "word": " أكتر", "probability": 0.9918212890625}, {"start": 2150.79, "end": 2151.05, "word": " من", "probability": 0.9970703125}, {"start": 2151.05, "end": 2151.47, "word": " عدد", "probability": 0.9970703125}, {"start": 2151.47, "end": 2152.09, "word": " الخلايا", "probability": 0.9871419270833334}, {"start": 2152.09, "end": 2152.63, "word": " الحية", "probability": 0.991455078125}], "temperature": 1.0}, {"id": 81, "seek": 216419, "start": 2153.5, "end": 2164.2, "text": "طبعا هذا ال curve هو المظبوط أكتر من ال curve اللي مار معناه لأنه موضح كل المراحل أو انبين عدد الخلايا بشكل أفضل", "tokens": [9566, 3555, 3615, 995, 23758, 2423, 7605, 31439, 9673, 19913, 3555, 2407, 9566, 5551, 4117, 2655, 2288, 9154, 2423, 7605, 13672, 1829, 3714, 9640, 20449, 8315, 3224, 5296, 33456, 3224, 3714, 2407, 11242, 5016, 28242, 9673, 23557, 5016, 1211, 34051, 16472, 3555, 9957, 6225, 3215, 3215, 33962, 15040, 25528, 4724, 8592, 28820, 5551, 5172, 11242, 1211], "avg_logprob": -0.11239035401427955, "compression_ratio": 1.443609022556391, "no_speech_prob": 0.0, "words": [{"start": 2153.5, "end": 2153.86, "word": "طبعا", "probability": 0.83905029296875}, {"start": 2153.86, "end": 2154.14, "word": " هذا", "probability": 0.486572265625}, {"start": 2154.14, "end": 2154.4, "word": " ال", "probability": 0.8603515625}, {"start": 2154.4, "end": 2154.7, "word": " curve", "probability": 0.28955078125}, {"start": 2154.7, "end": 2155.14, "word": " هو", "probability": 0.97509765625}, {"start": 2155.14, "end": 2156.5, "word": " المظبوط", "probability": 0.93544921875}, {"start": 2156.5, "end": 2156.88, "word": " أكتر", "probability": 0.938720703125}, {"start": 2156.88, "end": 2157.1, "word": " من", "probability": 0.9873046875}, {"start": 2157.1, "end": 2157.3, "word": " ال", "probability": 0.787109375}, {"start": 2157.3, "end": 2157.56, "word": " curve", "probability": 0.8974609375}, {"start": 2157.56, "end": 2157.7, "word": " اللي", "probability": 0.89404296875}, {"start": 2157.7, "end": 2157.96, "word": " مار", "probability": 0.90966796875}, {"start": 2157.96, "end": 2158.9, "word": " معناه", "probability": 0.9637044270833334}, {"start": 2158.9, "end": 2159.36, "word": " لأنه", "probability": 0.9269205729166666}, {"start": 2159.36, "end": 2159.78, "word": " موضح", "probability": 0.97021484375}, {"start": 2159.78, "end": 2160.16, "word": " كل", "probability": 0.98095703125}, {"start": 2160.16, "end": 2161.16, "word": " المراحل", "probability": 0.977783203125}, {"start": 2161.16, "end": 2161.72, "word": " أو", "probability": 0.912109375}, {"start": 2161.72, "end": 2162.26, "word": " انبين", "probability": 0.7443033854166666}, {"start": 2162.26, "end": 2162.62, "word": " عدد", "probability": 0.9879557291666666}, {"start": 2162.62, "end": 2163.24, "word": " الخلايا", "probability": 0.9708658854166666}, {"start": 2163.24, "end": 2163.72, "word": " بشكل", "probability": 0.9928385416666666}, {"start": 2163.72, "end": 2164.2, "word": " أفضل", "probability": 0.962646484375}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 2165.6555, "duration_after_vad": 2106.169999999993}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/Pl7CGd4BP8U_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/Pl7CGd4BP8U_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..a22f3b23f1ac34d1d2ccb13e5b204d1afd6d78a9
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/Pl7CGd4BP8U_postprocess.srt
@@ -0,0 +1,2616 @@
+1
+00:00:01,790 --> 00:00:05,850
+السلام عليكم ورحمة الله وبركاته نستكمل معاكم
+
+2
+00:00:05,850 --> 00:00:10,670
+محاضرات مساق الميكرو بيولوجي العملي في هذا اللقاء
+
+3
+00:00:10,670 --> 00:00:15,030
+هتكون المحاضرة بعنوان bacteria oxygen requirements
+
+4
+00:00:15,030 --> 00:00:19,790
+and anaerobic bacteria قلنا إن البكتيريا عشان تنمو
+
+5
+00:00:19,790 --> 00:00:25,550
+وتتكثر لازم أني أنا أوفر الظروف المناسبة للنمو من
+
+6
+00:00:25,550 --> 00:00:31,040
+هذه الظروف حاجتها للأكسجينهنتعرف في هذه المحاضرة
+
+7
+00:00:31,040 --> 00:00:35,940
+على تصنيفات البكتيريا حسب حاجتها للأكسجين وكيف أنا
+
+8
+00:00:35,940 --> 00:00:40,780
+بدي أقدر أوفر هذه الظروف عشان أقدر أنمي البكتيريا
+
+9
+00:00:40,780 --> 00:00:44,460
+introduction
+
+10
+00:00:44,460 --> 00:00:48,200
+بكتيريا can exist in a wide variety of
+
+11
+00:00:48,200 --> 00:00:51,560
+environments including environments that lack
+
+12
+00:00:51,560 --> 00:00:56,690
+oxygenبقولي إن البكتيريا ممكن إنها تنمو في بيئات
+
+13
+00:00:56,690 --> 00:01:01,930
+مختلفة من ضمن هذه البيئات البيئة اللي مش موجود
+
+14
+00:01:01,930 --> 00:01:06,050
+فيها أكسجين in fact أكسجين is toxic to some
+
+15
+00:01:06,050 --> 00:01:10,330
+bacteria بقولي في الحقيقة الأكسجين يعتبر toxic
+
+16
+00:01:10,330 --> 00:01:16,750
+some لبعض البكتيريا the toxicity is usually due to
+
+17
+00:01:16,750 --> 00:01:20,890
+accidental formation of superoxide and hydrogen
+
+18
+00:01:20,890 --> 00:01:24,700
+peroxidewhich react with other cell components
+
+19
+00:01:24,700 --> 00:01:29,820
+بقولّي أن الـ Oxygen toxic لبعض أنواع البكتيريا
+
+20
+00:01:29,820 --> 00:01:34,600
+قلنا أن الـ Oxygen هو المستقبل النهائي للإلكترونات
+
+21
+00:01:34,600 --> 00:01:40,400
+في الـ aerobic respiration في التنفس الخلوي طبعا
+
+22
+00:01:40,400 --> 00:01:45,380
+في التنفس الخلوي بيرتبط الإلكترون طبعا فيها أنا
+
+23
+00:01:45,380 --> 00:01:49,560
+عندي في الـ electron transport إدشانهي في ندي
+
+24
+00:01:49,560 --> 00:01:55,840
+مضخات تمام لالإلكترونات هيرتبط إلكترون مع اللي هو
+
+25
+00:01:55,840 --> 00:02:01,880
+ال oxygen مع المستقبل النهائي لالإلكترونات هيتكون
+
+26
+00:02:01,880 --> 00:02:07,900
+super oxide هيتكون super oxide اللي هو O2 minus
+
+27
+00:02:07,900 --> 00:02:14,480
+هذا مكتسب إلكترون يعتبر هذا toxicتمام لأنه بيقول
+
+28
+00:02:14,480 --> 00:02:17,940
+يها that this toxicity is usually من ون جاية high
+
+29
+00:02:17,940 --> 00:02:22,520
+toxicity من formation of super oxide كيف بتكون ال
+
+30
+00:02:22,520 --> 00:02:26,000
+super oxide خلال عملية ال aerobic respiration
+
+31
+00:02:26,000 --> 00:02:32,540
+المستقبل أنهيه إلى الإلكترونات هو ال oxygen تمام
+
+32
+00:02:32,540 --> 00:02:36,000
+ال oxygen هو المستقبل أنهيه إلى الإلكترونات هيرتبط
+
+33
+00:02:36,000 --> 00:02:40,320
+مع إلكترون و هيكون ال super oxide طبعا هذا يعتبر
+
+34
+00:02:40,320 --> 00:02:46,010
+toxicبيتم التخلص منه حبر إنزيم الـ super oxide
+
+35
+00:02:46,010 --> 00:02:51,350
+dismutase اتكلمنا في معمل أو chapter الـ Catalysis
+
+36
+00:02:51,350 --> 00:02:56,170
+Test انه انا بتخلص من ال super oxide عن طريق إنزيم
+
+37
+00:02:56,170 --> 00:03:02,830
+ال super oxide dismutase رح يكسره لH2O2
+
+38
+00:03:02,830 --> 00:03:06,550
+اللي هو ال hydrogen peroxide كيف انا بدي اتخلص من
+
+39
+00:03:06,550 --> 00:03:10,630
+ال hydrogen peroxide اللي يعتبر ايضا toxic و سام
+
+40
+00:03:10,960 --> 00:03:16,860
+بيتم التخلص منه اما عن طريق انزيم الكتلاز او عن
+
+41
+00:03:16,860 --> 00:03:21,480
+طريق انزيم الهيدروجين بيروكسيداز طبعا في معادلات
+
+42
+00:03:21,480 --> 00:03:27,240
+شرحناها سابقا شهو نواتج عملية تكسير الهيدروجين
+
+43
+00:03:27,240 --> 00:03:31,540
+بيروكسايد من الكتلاز او بواسطة انزيم الكتلاز و
+
+44
+00:03:31,540 --> 00:03:35,740
+بواسطة انزيم الهيدروجين بيروكسيداز و هنذكره في ال
+
+45
+00:03:35,740 --> 00:03:41,900
+slidات الجائعة تمام هذا بالنسبة لإنه من وين الـ
+
+46
+00:03:41,900 --> 00:03:45,220
+toxicity جاية من تكوين الـ super oxide والهيدروجين
+
+47
+00:03:45,220 --> 00:03:50,900
+بيروكسال بالنسبة للبكتيريا اللي ما عندها هذه
+
+48
+00:03:50,900 --> 00:03:54,180
+الانزيمات اللي ما عندها انزيم ال super oxide
+
+49
+00:03:54,180 --> 00:03:59,180
+dismutase أو الكتلاز أو الهيدروجين بيروكسداز هيكون
+
+50
+00:03:59,180 --> 00:04:05,260
+ال oxygen بالنسبة لها toxic فبالتالي مش هتلجأ
+
+51
+00:04:05,260 --> 00:04:10,590
+لظروف الهوائية مش هتستخدمالـ Aerobic Respiration
+
+52
+00:04:10,590 --> 00:04:16,450
+للحصول للطاقة هتلجأ للظروف اللاهوائية هتلجأ للان
+
+53
+00:04:16,450 --> 00:04:21,750
+Aerobic Respiration هتستخدم inorganic molecule غير
+
+54
+00:04:21,750 --> 00:04:27,050
+الأكسجين كمستقبل نهائي للإلكترونارoxygen species
+
+55
+00:04:27,050 --> 00:04:31,430
+is harmful if bacteria have no enzyme system to
+
+56
+00:04:31,430 --> 00:04:35,550
+eliminate it قولي ال oxygen يعتبر harmful لو
+
+57
+00:04:35,550 --> 00:04:39,610
+البكتيريا ما عندها الانزيمات عشان تتخلص من ال
+
+58
+00:04:39,610 --> 00:04:42,890
+toxic form of oxygen زي ال super oxide و ال
+
+59
+00:04:42,890 --> 00:04:46,470
+hydrogen peroxide إذا ما كان عندها انزيم ال super
+
+60
+00:04:46,470 --> 00:04:50,550
+oxide ديسميوتاز أو انزيم الكتلاز أو ال hydrogen
+
+61
+00:04:50,550 --> 00:04:54,490
+peroxidase هذا هيعتبر بالنسبة لإلها ال oxygen
+
+62
+00:04:54,490 --> 00:05:00,400
+toxicتاني معلومة ان ال oxygen بيكون harmful لو كان
+
+63
+00:05:00,400 --> 00:05:03,860
+ال oxygen species concentration is very high in
+
+64
+00:05:03,860 --> 00:05:09,480
+media or air لو كانت كمية ال oxygen أكتر من الكمية
+
+65
+00:05:09,480 --> 00:05:13,520
+اللي بتحتاجها البكتيريا فبالتالي هان بيكون هو
+
+66
+00:05:13,520 --> 00:05:20,200
+toxic some من نسبة لا البكتيرياطبعاً الـ Oxygen في
+
+67
+00:05:20,200 --> 00:05:27,980
+الـ Air نسبة الـ Oxygen في الهواء 21% إذا كان نسبة
+
+68
+00:05:27,980 --> 00:05:30,860
+الـ Oxygen أعلى موجودة في الـ media أو في الهواء
+
+69
+00:05:30,860 --> 00:05:36,840
+عامة بتحتاجه البكتيريا يعتبر toxic فبالتالي في هذه
+
+70
+00:05:36,840 --> 00:05:41,740
+الحالتين لو البكتيريا ما عندها انزيمات لاتخلص من
+
+71
+00:05:41,740 --> 00:05:46,470
+الـ Oxygen أو من الـ toxic form of Oxygenأو لو كان
+
+72
+00:05:46,470 --> 00:05:50,730
+نسبة الأكسجين في الميديا أو في الهواء أعلى من حاجة
+
+73
+00:05:50,730 --> 00:05:54,430
+أو أعلى من الكمية اللى بتحتاجها البكتيريا هيكون
+
+74
+00:05:54,430 --> 00:05:59,210
+toxic في هذه الحالات أكيد البكتيريا هتلجأ للظروف
+
+75
+00:05:59,210 --> 00:06:05,850
+اللاهوائية للحصول على الطاقة طيب نيجي لاقى ال
+
+76
+00:06:05,850 --> 00:06:13,310
+toxic form of oxygenأول form لأول شكل من أشكال ال
+
+77
+00:06:13,310 --> 00:06:18,590
+oxygen اللي بتكون toxic single oxygen بقول لي is
+
+78
+00:06:18,590 --> 00:06:22,930
+in high energy state extremely reactive exist in
+
+79
+00:06:22,930 --> 00:06:27,970
+phagocyte بقولي هذا هو يعتبر high energy state
+
+80
+00:06:27,970 --> 00:06:33,590
+بيكون extremely reactive نشط بتستخدمه بيتم
+
+81
+00:06:33,590 --> 00:06:38,830
+استخدامه في phagocytosis في البلعمةطبعا الخلايا
+
+82
+00:06:38,830 --> 00:06:45,330
+اللي هي الـ phagocyte لما اتهاجم الميكروب أو
+
+83
+00:06:45,330 --> 00:06:50,250
+البكتيريا وتعمله ابتلاع phagocytosis بتفرز عليه
+
+84
+00:06:50,250 --> 00:06:56,190
+الـ O2 بتفرز عليه single oxygen فبالتالي بيتهاج
+
+85
+00:06:56,190 --> 00:07:00,850
+بتموت البكتيريا أو الميكروب هذا بالنسبة لأول toxic
+
+86
+00:07:00,850 --> 00:07:05,210
+form of oxygenتاني toxic form الـ super oxide free
+
+87
+00:07:05,210 --> 00:07:09,830
+radical والهيدروجين بيروكسايد اللي بيتم إنتاجهم
+
+88
+00:07:09,830 --> 00:07:14,070
+formed in small amount in cellular respiration
+
+89
+00:07:14,070 --> 00:07:18,170
+اللي بيتم إنتاجهم في الcellular respiration أو في
+
+90
+00:07:18,170 --> 00:07:23,560
+ال aerobic respirationهدول so toxic to cell طبعا
+
+91
+00:07:23,560 --> 00:07:28,560
+هدول يعتبروا سمين بيتم التخلص منهم عن طريق طبعا
+
+92
+00:07:28,560 --> 00:07:32,500
+بالنسبة لل super oxide ال free radical بواسطة
+
+93
+00:07:32,500 --> 00:07:36,980
+أنزيم ال super oxide this imutase هيتكون ال
+
+94
+00:07:36,980 --> 00:07:39,480
+hydrogen peroxide كيف بنتخلص من ال hydrogen
+
+95
+00:07:39,480 --> 00:07:44,140
+peroxide إما بواسطة أنزيم الكتلاز أو بواسطة أنزيم
+
+96
+00:07:44,140 --> 00:07:48,500
+ال hydrogen peroxidaseطبعا الـ aerobic respiration
+
+97
+00:07:48,500 --> 00:07:55,580
+اللي هي عملية التنفس الهوائي الخلوي بما أنها نمت
+
+98
+00:07:55,580 --> 00:08:00,520
+في الظروف الهوائية لازم يكون عندها هاي الانزيمات
+
+99
+00:08:00,520 --> 00:08:04,820
+اللي اتكلمنا عنها عشان تتخلص من ال toxic form of
+
+100
+00:08:04,820 --> 00:08:09,520
+oxygen من هاي ال toxic form peroxide anion اللي هي
+
+101
+00:08:09,520 --> 00:08:15,440
+ال O2 لكن minus 2هذا هو super oxide free radical و
+
+102
+00:08:15,440 --> 00:08:20,660
+اكتسب كمان electron آخر used as antimicrobial
+
+103
+00:08:20,660 --> 00:08:25,060
+agent ايش يعني antimicrobial agent يعني مادة
+
+104
+00:08:25,060 --> 00:08:29,660
+تستخدم في قتل أو تثبيت البكتيري هذا معناه ال
+
+105
+00:08:29,660 --> 00:08:33,880
+antimicrobial agent اللي هنتكلم عنه بالتفصيل في
+
+106
+00:08:33,880 --> 00:08:41,410
+آخر chapter في المساقHydroxyl Hydroxyl Radical
+
+107
+00:08:41,410 --> 00:08:47,070
+اللي هو الـ OH- The most active form Form in the
+
+108
+00:08:47,070 --> 00:08:51,310
+cytoplasm from respiration طبعا هو ال most active
+
+109
+00:08:51,310 --> 00:08:56,530
+form و بيكون موجود في ال cytoplasm هذا بالنسبة لل
+
+110
+00:08:56,530 --> 00:09:03,530
+toxic form of oxygen نيجي لكيف أنا بدي أزيل هاي
+
+111
+00:09:03,530 --> 00:09:09,780
+السمية بقول لي toxic form of oxygenممكن اني او انا
+
+112
+00:09:09,780 --> 00:09:15,840
+بحتاج اني انا ازيل هاي السمية ال toxicity لهاي ال
+
+113
+00:09:15,840 --> 00:09:20,320
+form of oxygen باستخدام انزيمات قولنا ال super
+
+114
+00:09:20,320 --> 00:09:26,700
+oxide dismutes بتخلص من اللي هو ال free radical ال
+
+115
+00:09:26,700 --> 00:09:32,900
+O2 minus super oxide free radical الكتلاز بتخلص من
+
+116
+00:09:32,900 --> 00:09:36,320
+ال hydrogen peroxide ال peroxidase بتخلص من ال
+
+117
+00:09:36,320 --> 00:09:41,580
+hydrogen أيضابيروكسايد إذا لم يكن الميكروب ينتج
+
+118
+00:09:41,580 --> 00:09:45,540
+هذه الانزيمات يجب أن يكون لها حالة ايوروبية بقول
+
+119
+00:09:45,540 --> 00:09:50,320
+لو البكتيريا او الميكروب ماعنده هذه الانزيمات لازم
+
+120
+00:09:50,320 --> 00:09:55,420
+البكتيريا تلجأ للظروف اللاهوائية للحصول على الطاقة
+
+121
+00:09:55,420 --> 00:09:58,440
+الخلايا اللي يعيشون في حياتة محتوى أكسجين تحتوي
+
+122
+00:09:58,440 --> 00:10:05,730
+على انزيم سوبر إكسايد ديسميوتاز وكتاليزconvert
+
+123
+00:10:05,730 --> 00:10:09,130
+super oxide and peroxide to oxygen and water
+
+124
+00:10:09,130 --> 00:10:14,430
+بقوللي ان الخلايا اللي بتقدر تعيش في بيئة فيها
+
+125
+00:10:14,430 --> 00:10:18,690
+اكسجين اكيد هتمتلك انزيم ال super oxide ال
+
+126
+00:10:18,690 --> 00:10:23,050
+decimutase و ال catalase طبعا هتحول ال super oxide
+
+127
+00:10:23,050 --> 00:10:27,410
+و ال peroxide ال peroxide هذول طبعا ال toxic form
+
+128
+00:10:27,410 --> 00:10:32,880
+of اكسجين ل oxygen and waterحسب المعادلات اللى
+
+129
+00:10:32,880 --> 00:10:37,960
+هنشوفها في ال slide اللى بعده طبعا ال oxygen و ال
+
+130
+00:10:37,960 --> 00:10:42,700
+water هدول non-toxic form فبالتالي هيك زالت سمية
+
+131
+00:10:42,700 --> 00:10:48,740
+ال ال super oxide ال super oxide و ال peroxide او
+
+132
+00:10:48,740 --> 00:10:53,680
+ال hydrogen peroxide هاي بالنسبة للمعادلات ال
+
+133
+00:10:53,680 --> 00:10:57,920
+super oxide dismutase هذا الانزين بيحول ال super
+
+134
+00:10:57,920 --> 00:11:03,790
+oxide ions طبعاأحنا قلنا الـ super oxide اللي هو
+
+135
+00:11:03,790 --> 00:11:07,470
+الـ free radical هذا يعتبر toxic فعل فبالتالي كيف
+
+136
+00:11:07,470 --> 00:11:10,250
+أنا بدي أتخلص منه في عندي أنا في ال electron a
+
+137
+00:11:10,250 --> 00:11:14,850
+transported chain في عندي hydrogen ion هيتم
+
+138
+00:11:14,850 --> 00:11:18,430
+الارتباط مع ال hydrogen ionباستخدام إنزيم الـ
+
+139
+00:11:18,430 --> 00:11:23,490
+superoxide desimutase هيتكون عندى H2O2 زائد O2
+
+140
+00:11:23,490 --> 00:11:30,410
+قولنا بتكون بالنهاية water و oxygen هذه الـ H2O2
+
+141
+00:11:30,410 --> 00:11:34,630
+تعتبر toxic form كيف أتخلص منها إما باستخدام إنزيم
+
+142
+00:11:34,630 --> 00:11:39,250
+الكتلازأو باستخدام انزيم البروكسيداز بتكسر
+
+143
+00:11:39,250 --> 00:11:43,470
+الكتالياز بتكسر ال hydrogen peroxide اللى تم انتجه
+
+144
+00:11:43,470 --> 00:11:47,050
+أو تم تكوينه من ال free radical اللى هي ال super
+
+145
+00:11:47,050 --> 00:11:51,690
+oxide مع ال hydrogen ion هيتم تكسيره ال hydrogen
+
+146
+00:11:51,690 --> 00:11:56,830
+peroxide بواسطة انزيم الكتالياز ل water and oxygen
+
+147
+00:11:56,830 --> 00:12:01,010
+هيك انا اقدرت اتخلص من ال toxic form of oxygen
+
+148
+00:12:02,200 --> 00:12:06,520
+بالنسبة لـ Pyroxidase نفس الكتالياز بيشتغل على الـ
+
+149
+00:12:06,520 --> 00:12:10,140
+hydrogen pyroxide لكن الـ Pyroxidase أو الـ
+
+150
+00:12:10,140 --> 00:12:14,400
+hydrogen pyroxidase بيحول الـ hydrogen أو بيكسر
+
+151
+00:12:14,400 --> 00:12:20,380
+الـ hydrogen الـ pyroxide ل 2 مليكيول من الميه
+
+152
+00:12:20,380 --> 00:12:26,400
+2H2O يبقى بالنهاية اتكون عندي water و oxygen non
+
+153
+00:12:26,400 --> 00:12:31,060
+-toxic farm يقدر ان انا اتخلص من سمية ال oxygen
+
+154
+00:12:39,020 --> 00:12:44,060
+نيجي لل Oxygen requirement classification تصنيف
+
+155
+00:12:44,060 --> 00:12:48,780
+البكتيريا حسب حاجتها للأكسجين وقلي تم تصنيف
+
+156
+00:12:48,780 --> 00:12:54,460
+البكتيريا حسب حاجتها للأكسجين لخمس تصنيفات أول
+
+157
+00:12:54,460 --> 00:12:58,520
+تصنيف ال obligate Europe طبعا هاي ال obligate
+
+158
+00:12:58,520 --> 00:13:04,760
+Europe معناها هوائية إجبارية require oxygen to
+
+159
+00:13:04,760 --> 00:13:11,930
+liveبتحتاج أو بتستخدم ال oxygen حتى انها تقدر تعيش
+
+160
+00:13:11,930 --> 00:13:19,890
+يبقى هي بتحتاج لل oxygen حتى تقدر تعيش بتستخدمه
+
+161
+00:13:19,890 --> 00:13:26,690
+كمستقبل نهائي للإلكترونات للحصول على الطاقة their
+
+162
+00:13:26,690 --> 00:13:30,480
+metabolic pathway require oxygenas a final
+
+163
+00:13:30,480 --> 00:13:33,600
+electron acceptor خلال الـ aerobic respiration
+
+164
+00:13:33,600 --> 00:13:39,720
+يبقى هي بتستخدم ال aerobic respiration للحصول على
+
+165
+00:13:39,720 --> 00:13:44,280
+الطاقة لأن ال oxygen هو المستقبل النهائي
+
+166
+00:13:44,280 --> 00:13:48,900
+للإلكترونات في عندي طريقة أخرى للحصول على الطاقة
+
+167
+00:13:48,900 --> 00:13:53,320
+بقولي they have no alternate means of producing
+
+168
+00:13:53,320 --> 00:13:56,900
+ATP بقولي ماعندي أي طريقة أخرى للحصول على الطاقة
+
+169
+00:13:56,900 --> 00:14:01,630
+غير ال aerobic respirationلأن هي بتحتاج ال oxygen
+
+170
+00:14:01,630 --> 00:14:05,310
+حتى انها تقدر اتعيش فبالتالي هتستخدم ال oxygen
+
+171
+00:14:05,310 --> 00:14:13,250
+كمستقبل نهائي للإلكترونات طيب بما انها هوائية بما
+
+172
+00:14:13,250 --> 00:14:18,490
+انها هوائية تستخدم ال oxygen كمستقبل نهائي
+
+173
+00:14:18,490 --> 00:14:23,170
+للإلكترونات أكيد راح يكون عندها كل الانزيمات اللي
+
+174
+00:14:23,170 --> 00:14:28,050
+ذكرناها عشان تتخلص من ال toxic form of oxygen عشان
+
+175
+00:14:28,050 --> 00:14:31,820
+تتخلص منالـ Super Oxide الـ Free Radical من الـ
+
+176
+00:14:31,820 --> 00:14:35,340
+Hydrogen Peroxide اللي يتم تكوينهم خلال الـ
+
+177
+00:14:35,340 --> 00:14:39,680
+Aerobic Respiration طيب لو أنا زرعت بكتيريا
+
+178
+00:14:39,680 --> 00:14:44,820
+Obligate Aerobic بكتيريا في برث التيوب راح تنمو
+
+179
+00:14:44,820 --> 00:14:49,660
+البكتيريا بشكل كبير على السطح لأنه في نسبة
+
+180
+00:14:49,660 --> 00:14:54,020
+الأكسوجين على السطح بتكون أعلى ما يكون فهلاحظ إذا
+
+181
+00:14:54,020 --> 00:14:58,970
+كانت البكتيريا Obligate Aerobicهلاحظ أنه النمو
+
+182
+00:14:58,970 --> 00:15:03,490
+البكتيريا هيكون على السطح لأن أعلى نسبة Oxygen
+
+183
+00:15:03,490 --> 00:15:08,430
+بتكون في السطح هذا بالنسبة لأول تصنيف اللي هو
+
+184
+00:15:08,430 --> 00:15:12,310
+Obligate Aerobic Bacteria قولنا بتستخدم Aerobic
+
+185
+00:15:12,310 --> 00:15:16,950
+فقط Respiration للحصول على الطاقة تاني إشي عندها
+
+186
+00:15:16,950 --> 00:15:20,170
+كل الإنزيمات اللي تتخلص من ال toxic form of Oxygen
+
+187
+00:15:20,170 --> 00:15:24,960
+بتنمو علىالسطح طبعا لو أنا نميتها في broth ال tube
+
+188
+00:15:24,960 --> 00:15:30,220
+بتحتاج ال oxygen عشان تقدر اتقش بالنسبة للتصنيف
+
+189
+00:15:30,220 --> 00:15:34,500
+التاني ال facultative anaerobic bacteria اللي هي
+
+190
+00:15:34,500 --> 00:15:40,140
+اللا هوائية اختيارية بقول لي it grows in best
+
+191
+00:15:40,140 --> 00:15:45,700
+where most oxygen is presentedبقول لي بتنمو بوجود
+
+192
+00:15:45,700 --> 00:15:50,440
+ال oxygen او عدمه لكن تفضل وجود ال oxygen يبقى
+
+193
+00:15:50,440 --> 00:15:56,640
+بتقدر تنمو بوجود ال oxygen او في حال عدم وجوده لكن
+
+194
+00:15:56,640 --> 00:16:03,140
+هي بتفضل وجود ال oxygen او بتفضل انها تنمو في وجود
+
+195
+00:16:03,140 --> 00:16:08,420
+ال oxygen يبقى عشان احصل على الطاقة بما انها هي
+
+196
+00:16:08,420 --> 00:16:13,200
+هوائية فبحصل على الطاقة من خلالالـ aerobic
+
+197
+00:16:13,200 --> 00:16:24,440
+respiration يبقى تستهلك الأكسجين للحصول
+
+198
+00:16:24,440 --> 00:16:28,900
+على الطاقة لأنها هوائية تمام بتستخدم الأكسجين
+
+199
+00:16:28,900 --> 00:16:34,560
+للحصول على أو كمستقبل نهائي للإلكترونات طيب إذا ما
+
+200
+00:16:34,560 --> 00:16:40,870
+توفر الأكسجين قلنا بتقدر إنها تعيش طبعافي عدم وجود
+
+201
+00:16:40,870 --> 00:16:44,970
+الأكسجين فبالتالي هتستخدم الـ anaerobic
+
+202
+00:16:44,970 --> 00:16:50,470
+respiration و ال fermentation للحصول على الطاقة في
+
+203
+00:16:50,470 --> 00:16:58,610
+حال عدم وجود الأكسجين تمام طيب بالنسبة للإنزيمات
+
+204
+00:16:58,610 --> 00:17:03,850
+بما أنها هي هوائية في الأصل فبالتالي عندها يكون كل
+
+205
+00:17:03,850 --> 00:17:09,030
+الإنزيمات للتخلص من ال toxic form of oxygen طيب
+
+206
+00:17:09,290 --> 00:17:14,570
+بالنسبة لو أنا نميتها على او في إبرث تيوب هلاحظ
+
+207
+00:17:14,570 --> 00:17:19,430
+انه هذه البكتيريا هتنمو في كل تيوب سواء على السطح
+
+208
+00:17:19,430 --> 00:17:23,950
+او في أسفل التيوب لأنه احنا قلنا هي لها وقية
+
+209
+00:17:23,950 --> 00:17:28,530
+اختيارية بتقدر تنمو في عدم وجود الأكسجين في حال
+
+210
+00:17:28,530 --> 00:17:33,210
+كان مش موجود الأكسجين بتقدر تنمو في حال عدم وجوده
+
+211
+00:17:33,210 --> 00:17:37,450
+يبقى هلاحظ انه في كل تيوب في انديو نمو لأ
+
+212
+00:17:37,450 --> 00:17:42,050
+البكتيريالكن النسبة الأعلى هتكون على السطح لأنها
+
+213
+00:17:42,050 --> 00:17:47,270
+هي قلنا بتفضل وجود ال oxygen هذا بالنسبة لتان
+
+214
+00:17:47,270 --> 00:17:51,710
+تصنيف ال facultative anaerobic bacteria نيجي
+
+215
+00:17:51,710 --> 00:17:56,070
+لتصنيف التالت اللي هو ال obligate anaerobic
+
+216
+00:17:56,070 --> 00:18:01,850
+bacteria اللاهوائية الإجبارية ال growth occurs
+
+217
+00:18:01,850 --> 00:18:06,390
+only when there is no oxygenبقول لي تنمو في غياب
+
+218
+00:18:06,390 --> 00:18:11,650
+الأكسجين فقط انه مو يحدث فقط عند عدم موجود
+
+219
+00:18:11,650 --> 00:18:17,170
+الأكسجين تمام طيب بقول لي انه use anaerobic
+
+220
+00:18:17,170 --> 00:18:21,030
+respiration او fermentation to generate ATP اكيد
+
+221
+00:18:21,030 --> 00:18:26,750
+بما انها لهوائية هتحصل على الطاقة من خلال ال
+
+222
+00:18:26,750 --> 00:18:30,570
+anaerobic respiration ومن خلال ال fermentation مش
+
+223
+00:18:30,570 --> 00:18:34,540
+هيتم استخدام ال anaerobic respirationفي الـ
+
+224
+00:18:34,540 --> 00:18:38,220
+obligate anaerobic لحصول على الطاقة فقط ال
+
+225
+00:18:38,220 --> 00:18:43,580
+anaerobic respiration و ال fermentation طيب ليش ما
+
+226
+00:18:43,580 --> 00:18:50,620
+بتقدر تنمو في وجود ال oxygen بقوللي هي بتفقد
+
+227
+00:18:50,620 --> 00:18:55,880
+الانزيمات اللي هذه الانزيمات من خلالها بنتخلص من
+
+228
+00:18:55,880 --> 00:19:00,560
+ال toxic form لال oxygen لإن سوبر اكساد ديسميوتاز
+
+229
+00:19:00,560 --> 00:19:05,180
+كتلاز بيركسدازفبالتالي هتموت في وجود ال Oxygen
+
+230
+00:19:05,180 --> 00:19:09,400
+يبقى ما عندها الانزيمات للتخلص من ال toxic form of
+
+231
+00:19:09,400 --> 00:19:15,920
+Oxygen فبالتالي مش هتقدر تنمو إلا في حال عدم موجود
+
+232
+00:19:15,920 --> 00:19:20,880
+ال Oxygen لو كان موجود ال Oxygen هتموت die in the
+
+233
+00:19:20,880 --> 00:19:25,100
+presence of Oxygen دي عدم موجود الانزيمات اللازمة
+
+234
+00:19:25,100 --> 00:19:29,540
+للتخلص من ال toxic form of Oxygen يبقى ما عندها
+
+235
+00:19:29,540 --> 00:19:36,110
+الانزيماتاللي ذكرناها لأنها لاهوائية طيب لو أنا
+
+236
+00:19:36,110 --> 00:19:39,890
+زراعت Obligate بكتيريا Obligate anaerobic في broth
+
+237
+00:19:39,890 --> 00:19:44,770
+tube فقط هلاقى النمو في الأسفل هيكون sediment لأن
+
+238
+00:19:44,770 --> 00:19:51,370
+هي تنمو في حال عدم وجود ال oxygen طب نيجي لرابع
+
+239
+00:19:51,370 --> 00:19:56,490
+تصنيف الـ Aerotolerant Anaerobic Bacteria هذه
+
+240
+00:19:56,490 --> 00:20:02,550
+البكتيريا هي بالاصل لاهوائيةلما احنا قلنا على ال
+
+241
+00:20:02,550 --> 00:20:07,010
+faculty of anaerobic قلنا البكتيريا هي هي لا
+
+242
+00:20:07,010 --> 00:20:11,970
+هوائية اختيارية هي في الاصل هوائية لكن في حال عدم
+
+243
+00:20:11,970 --> 00:20:18,230
+وجود ال oxygen ممكن انها تقدر تنمو وتتكثر لكن ال
+
+244
+00:20:18,230 --> 00:20:22,510
+aerotolerant anaerobic بكتيريا هي البكتيريا هي في
+
+245
+00:20:22,510 --> 00:20:29,650
+الاصل لا هوائية لكن بتتحمل وجود كمية قليلة من ال
+
+246
+00:20:29,650 --> 00:20:34,060
+oxygentolerate the presence of oxygen and can
+
+247
+00:20:34,060 --> 00:20:38,220
+survive in air but doesn't require it for growth
+
+248
+00:20:38,220 --> 00:20:43,620
+هي بتتحمل كمية معينة من ال oxygen لكن ما بتحتاجها
+
+249
+00:20:43,620 --> 00:20:48,600
+في النمو لأنه هي بالاصل بكتيريا لا هوائية طيب بما
+
+250
+00:20:48,600 --> 00:20:51,820
+أن هي بكتيريا لا هوائية عشان تحصل على الطاقة
+
+251
+00:20:51,820 --> 00:20:55,340
+بتستخدم الأن Europe crisp by ration وال
+
+252
+00:20:55,340 --> 00:21:00,400
+fermentation فقط لأن هي بالاصل لا هوائية مش هتقدر
+
+253
+00:21:00,680 --> 00:21:03,980
+بتستخدم الـ aerobic respiration للحصول على الطاقة
+
+254
+00:21:03,980 --> 00:21:07,160
+فقط الآن aerobic respiration وال fermentation هي
+
+255
+00:21:07,160 --> 00:21:12,520
+اللي هتحصل من خلالهم على الطاقة طيب، they cause
+
+256
+00:21:12,520 --> 00:21:17,560
+superoxide dismutase ليش بتقدر هذه البكتيريا تتحمل
+
+257
+00:21:17,560 --> 00:21:22,440
+كمية معينة من الأكسجين نتيجة وجود إنزيم ال
+
+258
+00:21:22,440 --> 00:21:27,700
+superoxide dismutaseفبالتالي ممكن انها تقدر تتحمل
+
+259
+00:21:27,700 --> 00:21:31,700
+كمية معينة لانه موجود فقط انزيم ال super oxide
+
+260
+00:21:31,700 --> 00:21:35,320
+dosimutase فبالتالي هتتخلص من ال toxic form of
+
+261
+00:21:35,320 --> 00:21:39,220
+أكسجين يبقى تحتوي فقط على هذا الانزيم عشان هيك
+
+262
+00:21:39,220 --> 00:21:44,440
+بتقدر تتحمل كمية معينة من الأكسجين لو زاد عن هذه
+
+263
+00:21:44,440 --> 00:21:51,000
+الكمية الأكسجين هتموت البكتيريا طيب لو انا بدي
+
+264
+00:21:51,000 --> 00:21:57,210
+أنمي هذه البكتيريا في ال tubeتمام هلاقي انه ممكن
+
+265
+00:21:57,210 --> 00:22:02,030
+تقدر تنمو على طبعا في كل tube لكن اكتر مكان هتنمو
+
+266
+00:22:02,030 --> 00:22:05,990
+فيه اللي هو تحت في الأسفل ال sediment هذا بالنسبة
+
+267
+00:22:05,990 --> 00:22:11,870
+لل aerotolerant aerobic bacteria اخر تصنيف اللي هو
+
+268
+00:22:11,870 --> 00:22:18,370
+ال micro aerobifillas اللي هي growth فقط بتنمو في
+
+269
+00:22:18,370 --> 00:22:22,430
+حال كان في عندي concentration قليل من الأكسجين
+
+270
+00:22:27,590 --> 00:22:32,350
+في حال كان عندى كمية قليلة من ال oxygen بتحتاج
+
+271
+00:22:32,350 --> 00:22:37,710
+كمية قليلة من ال oxygen عشان تنمو لكن مش الكمية
+
+272
+00:22:37,710 --> 00:22:41,550
+اللى بتحتاجها الهوائية الإجبارية احنا قولنا ان
+
+273
+00:22:41,550 --> 00:22:44,330
+الهوائية الإجبارية ال obligate aerobic bacteria
+
+274
+00:22:44,330 --> 00:22:48,890
+بتحتاج oxygen حتى تنمو هذه نفس الفكرة بتحتاج ال
+
+275
+00:22:48,890 --> 00:22:52,830
+oxygen حتى تنمو لكن بتحتاج كمية قليلة من ال oxygen
+
+276
+00:22:52,830 --> 00:22:57,940
+حتى تنموأقل من خمسة لعشرة في المية من ال oxygen
+
+277
+00:22:57,940 --> 00:23:03,360
+فقط حتى تنمو البكتيريا تحتاج كمية من خمسة لعشرة او
+
+278
+00:23:03,360 --> 00:23:07,580
+اقل من خمسة لعشرة في المية من ال oxygen عشان تقدر
+
+279
+00:23:07,580 --> 00:23:13,520
+تنمو و تتكثر طيب عشان احصل على الطاقة رح تحصل على
+
+280
+00:23:13,520 --> 00:23:18,160
+الطاقة فقط من ال aerobic respiration رح تنمو على
+
+281
+00:23:18,160 --> 00:23:24,040
+السطح فقط تمام او رح تنمو مش على السطحأسفل من
+
+282
+00:23:24,040 --> 00:23:28,200
+السطح بقليل يعني هيكون أقل من السطح مش على السطح
+
+283
+00:23:28,200 --> 00:23:32,920
+شوية تحت من السطح لأنه هي بتحتاج كمية قليلة من
+
+284
+00:23:32,920 --> 00:23:38,760
+الأكسجين راح تحتوي هي على كل أنواع الإنزيمات
+
+285
+00:23:38,760 --> 00:23:43,000
+اللازم من التخلص من ال toxic form بكتيريا هذا
+
+286
+00:23:43,000 --> 00:23:47,780
+بالنسبة لتصنيفات البكتيريا حسب حاجتها للأكسجين
+
+287
+00:23:53,740 --> 00:23:59,860
+البكتيريا الـ Cabinophilic
+
+288
+00:23:59,860 --> 00:24:06,880
+بيحتاج الـ CO2 حتى تنمو وتتكثرطب نيجي لهاي ال
+
+289
+00:24:06,880 --> 00:24:10,200
+slide هاي ال slide املخصت قريبا كل اللي ذكرنا
+
+290
+00:24:10,200 --> 00:24:15,300
+تصنيفات البكتيريا حسب حاجتها للأكسجين نيجي ل ال
+
+291
+00:24:15,300 --> 00:24:18,600
+obligate aerobic قولنا عشان تحصل على الطاقة من
+
+292
+00:24:18,600 --> 00:24:22,420
+خلال ال cellular respiration من خلال ال aerobic
+
+293
+00:24:22,420 --> 00:24:26,600
+respiration أو ال cellular respiration فقط للحصول
+
+294
+00:24:26,600 --> 00:24:32,120
+على الطاقةهي aerobic مش anaerobic لما اناميها في
+
+295
+00:24:32,120 --> 00:24:36,500
+broth tube هلاقي انه موفقت على السطح بالنسبة
+
+296
+00:24:36,500 --> 00:24:40,920
+للانزيمات كل الانزيمات موجودة عندها هي ال
+
+297
+00:24:40,920 --> 00:24:43,500
+bacterial obligate aerobic بكتيريا طبعا
+
+298
+00:24:43,500 --> 00:24:46,800
+البروكسيداز والكتاليز واحد منهم هيكون موجود مش
+
+299
+00:24:46,800 --> 00:24:50,140
+الاتنين واحد منهم البكتيريا اللي عندها الكتاليز مش
+
+300
+00:24:50,140 --> 00:24:54,170
+هيكون عندها البروكسيداز والعكس صحيحطب نيجي لأ الـ
+
+301
+00:24:54,170 --> 00:24:57,650
+facultative anaerobic bacteria قولنا هي لا هوائية
+
+302
+00:24:57,650 --> 00:25:03,990
+اختيارية هي بالاصل هوائية يبقى عشان تحصل على
+
+303
+00:25:03,990 --> 00:25:07,690
+الطاقة من خلال الـ Cellular Respiration وكمان
+
+304
+00:25:07,690 --> 00:25:13,370
+بتقدر تنمو في عدم موجود الـ Oxygen فبالتالي من
+
+305
+00:25:13,370 --> 00:25:16,450
+خلال الـ Anaerobic Respiration والـ Fermentation
+
+306
+00:25:16,450 --> 00:25:19,390
+بحصل على الطاقة يبقى بشكل عام الـ Respiration بـ
+
+307
+00:25:19,390 --> 00:25:22,450
+Cellular Run-aerobic respiration والـ fermentation
+
+308
+00:25:22,450 --> 00:25:26,790
+هتحصل على الطاقة هي aerobic وun-aerobic بكتيريا
+
+309
+00:25:26,790 --> 00:25:29,810
+قلنا بما أنها هي هوائية يبقى هيكون عندها كل
+
+310
+00:25:29,810 --> 00:25:34,110
+الانزيمات النمو في الـ breath tube هتكون في كل ال
+
+311
+00:25:34,110 --> 00:25:37,770
+tube لكن أكتر شئ هيكون على السطح لأنه هي قلنا
+
+312
+00:25:37,770 --> 00:25:43,510
+بالاصل هي هوائية بتحب أو تفضل وجود الأكسجين
+
+313
+00:25:43,510 --> 00:25:49,290
+بالنسبة لل obligate un-aerobic bacteriaهي بكتيريا
+
+314
+00:25:49,290 --> 00:25:52,470
+لا هوائية Anaerobic بكتيريا مش Aerobic يبقى
+
+315
+00:25:52,470 --> 00:25:55,770
+فبالتالي فقط عشان تحصل على الطاقة من خلال ال
+
+316
+00:25:55,770 --> 00:25:59,950
+fermentation أو من خلال ال anaerobic respiration
+
+317
+00:25:59,950 --> 00:26:04,410
+أو هان ذكرها ال autotrophy اللي هي ال anaerobic
+
+318
+00:26:04,410 --> 00:26:07,310
+respiration أكيد بما أنها anaerobic مش هيكون في
+
+319
+00:26:07,310 --> 00:26:10,690
+عندها ولا أنزيم من الانزيمات للتخلص من ال toxic
+
+320
+00:26:10,690 --> 00:26:14,710
+foreign بكتيريا هي بتمود في وجود ال oxygen نتيجة
+
+321
+00:26:14,710 --> 00:26:18,770
+عدمووجود الانزيمات للتخلص من الـ toxic form
+
+322
+00:26:18,770 --> 00:26:23,890
+للأكسجين نموها في البروستيتيوب هيكون في الأسفل في
+
+323
+00:26:23,890 --> 00:26:28,950
+ال sediment فقط مش هيكون في على السطح لأن هي بتنمو
+
+324
+00:26:28,950 --> 00:26:32,710
+فقط في وجود الأكسجينالـ Aerotolerant Anaerobic
+
+325
+00:26:32,710 --> 00:26:37,090
+قلنا هي في الأصل Anaerobic بكتيريا لهوائية لكن
+
+326
+00:26:37,090 --> 00:26:44,130
+بتتحمل اللي هو كمية قليلة من الأكسجين فقط بتتحمل
+
+327
+00:26:44,130 --> 00:26:47,870
+كمية قليلة من الأكسجين بتقدر نتيجة وشود ال super
+
+328
+00:26:47,870 --> 00:26:49,850
+oxide desimiotase enzyme
+
+329
+00:26:52,460 --> 00:26:56,880
+من خلال أو من خلال ال fermentation بتحصل على
+
+330
+00:26:56,880 --> 00:27:00,520
+الطاقة وكمان من خلال اللي هو ال unaerobic
+
+331
+00:27:00,520 --> 00:27:03,420
+respiration يبقى هان نضيف ال unaerobic respiration
+
+332
+00:27:03,420 --> 00:27:07,800
+بما أنها لا هوائية فبالتالي بتحصل على الطاقة من
+
+333
+00:27:07,800 --> 00:27:11,660
+خلال التنفس الخلوي أو من خلال التنفس اللاهوائي
+
+334
+00:27:11,660 --> 00:27:14,580
+اللي هو ال unaerobic respiration من خلال ال
+
+335
+00:27:14,580 --> 00:27:21,430
+fermentationهلاحظ أنه بالنسبة للـ Aerotolerant and
+
+336
+00:27:21,430 --> 00:27:28,790
+aerobic bacteria هتكون طبعا اللي هو فيه نموها
+
+337
+00:27:28,790 --> 00:27:35,130
+هيكون هي طبعا لا هوائية هيكون في كل tube أكتر إش
+
+338
+00:27:35,130 --> 00:27:39,290
+هيكون في الأسفل لأن هي لا هوائية لكن ممكن أني أنا
+
+339
+00:27:39,290 --> 00:27:43,510
+ألاقيها في الأعلى لأنه بتتحمل كمية معينة من ال
+
+340
+00:27:43,510 --> 00:27:50,550
+oxygenبالنسبة لأخر شيء ال micro aerophilic بكتيريا
+
+341
+00:27:50,550 --> 00:27:56,230
+طبعا عشان تحصل على الطاقة فقط من خلال ال cellular
+
+342
+00:27:56,230 --> 00:27:59,490
+respiration او ال aerobic respiration زي ال
+
+343
+00:27:59,490 --> 00:28:04,650
+obligate aerobic لأنها تحتاج للنمو تحتاج ال oxygen
+
+344
+00:28:04,650 --> 00:28:08,550
+لكن بكمية قليلة أقل مما تحتاج ال obligate aerobic
+
+345
+00:28:08,550 --> 00:28:14,350
+بكتيريا عشان هيك بشوفها أقلبقليل من مش على السطح
+
+346
+00:28:14,350 --> 00:28:21,170
+يعني أقل من السطح شوية لتحت من السطح بتنمو هي
+
+347
+00:28:21,170 --> 00:28:24,390
+aerobic فقط مش anaerobic موجود فيها كل الانزيمات
+
+348
+00:28:24,390 --> 00:28:28,730
+للتخلص من ال toxic form أمثلة على ال obligate
+
+349
+00:28:28,730 --> 00:28:32,030
+aerobic bacteria البصلص والصدومونص الفكولتات of
+
+350
+00:28:32,030 --> 00:28:35,250
+anaerobic bacteria كل ال enterobacterias بما فيهم
+
+351
+00:28:35,250 --> 00:28:39,780
+ال E.coli والستفيلوكوكسبالنسبة للـ Obligate
+
+352
+00:28:39,780 --> 00:28:43,080
+Anaerobic Bacteria عندي الـ Clostridium، الـ
+
+353
+00:28:43,080 --> 00:28:46,500
+Aerotolerant Anaerobic Bacteria الـ Lactobacillus،
+
+354
+00:28:46,500 --> 00:28:49,940
+الـ Micro Aerophilus اللي هي الـ Campylobacter و
+
+355
+00:28:49,940 --> 00:28:53,780
+الـ Helicobacter Biliary هذا بالنسبة للجدول طبعا
+
+356
+00:28:53,780 --> 00:28:58,660
+بالنسبة ل الأمثلة مطلوب منكوا حفظ الأمثلة ومهمين
+
+357
+00:28:58,660 --> 00:29:04,760
+هدال الأمثلةهذا تقريبا الجدول ملخص لكل اللي ذكرناه
+
+358
+00:29:04,760 --> 00:29:10,540
+عن تصنيفات البكتيريا حسب حاجتها للأكسجين طيب هلأ
+
+359
+00:29:10,540 --> 00:29:17,640
+نيجي للخطوات عشان أعرف شو تصنيفات البكتيريا حسب
+
+360
+00:29:17,640 --> 00:29:22,480
+حاجتها للأكسجين بقوللي أني أنا بستخدم ميديا brand
+
+361
+00:29:22,480 --> 00:29:26,700
+hard infusion agar في ندي إبروت لكن أنا بستخدم ال
+
+362
+00:29:26,700 --> 00:29:30,960
+brand hard infusion agarمكوناتها بيبتون mixed
+
+363
+00:29:30,960 --> 00:29:34,220
+chard البيبتون بيكون هو عبارة عن بروتين مصدر
+
+364
+00:29:34,220 --> 00:29:38,660
+للكربون والنيتروجين اللازم لنمو البكتيريا بيف hard
+
+365
+00:29:38,660 --> 00:29:42,380
+infusion طبعا بيف اللي هو اللحم البقري Gulf Brain
+
+366
+00:29:42,380 --> 00:29:47,280
+Infusion، Gulf يعني جل Sodium Chloride طبعا عشان
+
+367
+00:29:47,280 --> 00:29:51,860
+أحافظ على ال Osmotic Pressure Dipotassium
+
+368
+00:29:51,860 --> 00:29:55,000
+Phosphate اللي هو ال buffer عشان يعادل ال BH
+
+369
+00:29:55,000 --> 00:30:01,480
+Textrose طبعا اللي هو ال nutrientاللي هو السكر في
+
+370
+00:30:01,480 --> 00:30:05,640
+عندي اكيد بما انه هي agar اكيد هيكون نسبة agar 1.5
+
+371
+00:30:05,640 --> 00:30:10,920
+% عشان اعمل اللي هو او اشوف تصنيفات البكتيريا حسب
+
+372
+00:30:10,920 --> 00:30:17,360
+حاجتها للأكسجين بقوللي هتستخدمي اتيوبات او تلاتة
+
+373
+00:30:17,360 --> 00:30:23,040
+من brain heart infusion agar دي هستخدم اتيوبات
+
+374
+00:30:23,040 --> 00:30:26,860
+الاتيوبات هي هيكون مصبوب فيها media ل brain heart
+
+375
+00:30:26,860 --> 00:30:32,750
+infusion agarهصبها بـ deep هتكون deep اللي هو مش
+
+376
+00:30:32,750 --> 00:30:35,890
+هعملها طبعا بشكل مائل فقط بعد ما تطلع من الـ
+
+377
+00:30:35,890 --> 00:30:41,190
+autoclave هخليها تتصلب بشكل عادي بشكل مستقيم بدي
+
+378
+00:30:41,190 --> 00:30:45,050
+hot plate water bath ice water bath و thermometer
+
+379
+00:30:45,050 --> 00:30:52,550
+طيب شو الخطوات بقوللي بتجيبي اللي هو ال brain
+
+380
+00:30:52,550 --> 00:30:57,680
+heart infusion agar dips tubeهدول طبعا بعد ما انت
+
+381
+00:30:57,680 --> 00:31:01,860
+جهزتيهم و حضرت ال media و طبعا طلعتيهم من ال
+
+382
+00:31:01,860 --> 00:31:05,660
+Octoclav اتصلبوا و قلنا بنحط هاي ليتيوبات في
+
+383
+00:31:05,660 --> 00:31:11,020
+boiling water bath بنجهز water bath على درجة حرارة
+
+384
+00:31:11,020 --> 00:31:15,420
+اكتر من 100 طبعا boiling water bath يعني ان ال
+
+385
+00:31:15,420 --> 00:31:18,200
+water bath بتكون الميه بتغلي يعني درجة الحرارة
+
+386
+00:31:18,200 --> 00:31:23,330
+اكتر من 100بُلي after the brain hard infusion أجار
+
+387
+00:31:23,330 --> 00:31:27,970
+ملت أكيد بما أنه درجة الحرارة مية أكيد الأجار قلنا
+
+388
+00:31:27,970 --> 00:31:32,670
+بتصلب عند 85 درجة سليسيا فأكيد هيكون هيتصلب اللي
+
+389
+00:31:32,670 --> 00:31:38,360
+هو الأجار في هاي ال mediaبقول لي لما اتأكد انه ال
+
+390
+00:31:38,360 --> 00:31:44,800
+agar اللي هو داب او sorry هو طبعا بت بيدوب عند
+
+391
+00:31:44,800 --> 00:31:49,980
+درجة حرارة 85 في الدرجة السيليزياس بيدوب عند 85
+
+392
+00:31:49,980 --> 00:31:56,160
+درجة السيليزياس و بتصلب عند 45 فبالتالي اكيد عند
+
+393
+00:31:56,160 --> 00:32:01,100
+بما انه درجة الحرارة ال water bath مية اكيد هي هي
+
+394
+00:32:01,100 --> 00:32:07,800
+دوب هذا ال agarبقولي لما اتأكد ان الأجار داب بتخلي
+
+395
+00:32:07,800 --> 00:32:12,160
+اللي هم الليتيوبات في الراك او بتخلي ال water
+
+396
+00:32:12,160 --> 00:32:18,800
+يسيله boiling انه يغلي لمدة خمس دقائق تمام اقل
+
+397
+00:32:18,800 --> 00:32:24,390
+شيخمس دقائق ليش هذا الاشي ليش انا بخلي انهبحط
+
+398
+00:32:24,390 --> 00:32:30,670
+ليتيوبات و بخليه يغلي او بخلي خمس دقايق انه يتعرض
+
+399
+00:32:30,670 --> 00:32:35,410
+لدرجة حرارة عالية بقولي عشان اضمن انه تم طرد
+
+400
+00:32:35,410 --> 00:32:40,310
+الاكسجين من ليتيوب اذا كان فيه اي غازات اي اكسجين
+
+401
+00:32:40,310 --> 00:32:44,210
+فيه ليتيوبات يتم طردها باستخدام درجة الحرارة
+
+402
+00:32:44,210 --> 00:32:48,350
+العالية بقولي بعدين بتحطي طبعا thermometer في ال
+
+403
+00:32:48,350 --> 00:32:53,220
+water bath عشان نستراق بدرجة الحرارةلما طبعا انت
+
+404
+00:32:53,220 --> 00:32:58,340
+بتقللي درجة الحرارة او بتطفي الحرارة لما توصل
+
+405
+00:32:58,340 --> 00:33:02,160
+لدرجة حرارة خمسة و أربعين درجة سيريزيا اللي قلنا
+
+406
+00:33:02,160 --> 00:33:08,220
+بتصلب عندها الوات الأجار بقولي بتقومي لتيوبات من
+
+407
+00:33:08,220 --> 00:33:14,040
+ال water path وهنا لازم أنتبه انه ما احرق اللي هو
+
+408
+00:33:14,040 --> 00:33:18,200
+لتيوب عشان مايصير اي اختلاط ويدخل ال oxygen من
+
+409
+00:33:18,200 --> 00:33:24,210
+جديدبعد ما انا رفعت ال tube من ال water bath بقولي
+
+410
+00:33:24,210 --> 00:33:28,330
+بتعملي inculate each culture into separate agarde
+
+411
+00:33:28,330 --> 00:33:34,690
+كيف انا بدي اعمل inculate في تيوبات agarde من خلال
+
+412
+00:33:34,690 --> 00:33:37,970
+اللي هو ال stabbing بدي اعمل ال stabbing باستخدام
+
+413
+00:33:37,970 --> 00:33:42,130
+ال inculating needle بقولي حاولي انك تتجنبي ال
+
+414
+00:33:42,130 --> 00:33:47,460
+shaking او انه تعملي air bubbles في ال mediaبعد ما
+
+415
+00:33:47,460 --> 00:33:51,780
+اتخلصى على طول بتحطيهم فى ال incubation تمام او
+
+416
+00:33:51,780 --> 00:33:56,360
+قبل فى ال incubation بقولى بتحطيهم فى ال ice water
+
+417
+00:33:56,360 --> 00:34:01,860
+bath ليش عشان اضمن انه الاجار اتصلب completely
+
+418
+00:34:01,860 --> 00:34:05,000
+solid until they are completely solid عشان اضمن
+
+419
+00:34:05,000 --> 00:34:10,330
+انه الاجار اتصلبانكيوبات دي بعضها بتاخد ال tube
+
+420
+00:34:10,330 --> 00:34:14,770
+بتعمليلهم انكيوباشين على درجة حرارة 37 درجة
+
+421
+00:34:14,770 --> 00:34:22,770
+سيليسيوس لمدة 24 48 و 72 ساعة ليش 48 او 72 ساعة
+
+422
+00:34:22,770 --> 00:34:27,550
+لأن البكتيريا الهوائية بتنمو أسرع من اللي هوائية
+
+423
+00:34:27,550 --> 00:34:32,830
+البكتيريا اللي هوائية عشان تنمو بتحتاج من 48 ل 72
+
+424
+00:34:32,830 --> 00:34:39,090
+ساعةبعد ما اتخلص المدة ال incubation بقولي بتشوفي
+
+425
+00:34:39,090 --> 00:34:44,990
+اللي هو نمو البكتيريا هلاحظ ان ال obligate aerobic
+
+426
+00:34:44,990 --> 00:34:48,890
+bacteria هتنمو فقط على الصدح اللي سميناها اللي هي
+
+427
+00:34:48,890 --> 00:34:52,450
+ال bellicle ال faculty of anaerobic bacteria قلنا
+
+428
+00:34:52,450 --> 00:34:56,910
+هتنمو في كل تيوب لكن اكتر شئ هشوفه على الصدح ال
+
+429
+00:34:56,910 --> 00:35:00,270
+obligate anaerobic bacteria فقط في البطم اللي هي
+
+430
+00:35:00,270 --> 00:35:04,240
+ال sedimentالـ Aerotolerant anaerobic بكتيريا قلنا
+
+431
+00:35:04,240 --> 00:35:09,900
+هيكون طبعا في كل tube لكن أكتر شئ هيكون تحت لأن هي
+
+432
+00:35:09,900 --> 00:35:16,640
+بالاصل لا هوائية ال micro aerophilic بكتيريا اللي
+
+433
+00:35:16,640 --> 00:35:21,600
+هي قلنا بتنمو في وجود ال oxygen لكن بتحتاج كمية
+
+434
+00:35:21,600 --> 00:35:26,520
+قليلة فبالتالي هتكون أسفل من أسفل السطح بشوية هذا
+
+435
+00:35:26,520 --> 00:35:31,100
+بالنسبة لتصنيفات البكتيريا حسب حاجتها لل oxygen
+
+436
+00:35:36,550 --> 00:35:40,150
+الان يوربك بكتيريا كيف بدي أنمي البكتيريا
+
+437
+00:35:40,150 --> 00:35:44,190
+اللاهوائية في عندي أكتر من طريقة عشان أنمي الان
+
+438
+00:35:44,190 --> 00:35:47,490
+يوربك بكتيريا ونلاحظ ان الان يوربك بكتيريا بتنمو
+
+439
+00:35:47,490 --> 00:35:52,950
+في الأسفل اللي هي سميناها ال sedimentفي هاي ال
+
+440
+00:35:52,950 --> 00:35:56,830
+slide ذاكر اللي هو او عامل مقارنة بين الـ
+
+441
+00:35:56,830 --> 00:36:00,470
+anaerobic bacteria و ال aerobic or facultative
+
+442
+00:36:00,470 --> 00:36:05,930
+anaerobic bacteria بالنسبالي ال anaerobic bacteria
+
+443
+00:36:05,930 --> 00:36:11,970
+بقولي ال oxygen طبعا طبعا انا في عندي toxic او في
+
+444
+00:36:11,970 --> 00:36:16,870
+عندي اللي هو toxic form لال oxygen قلنا همهي كل
+
+445
+00:36:16,870 --> 00:36:19,930
+الـ toxic form بقوللي في الـ anaerobic bacteria ما
+
+446
+00:36:19,930 --> 00:36:26,810
+بقدر اتخلص من الـ toxic form للأكسجين no detoxify
+
+447
+00:36:26,810 --> 00:36:31,050
+pathway مافي عندي أي طرق لإزالة سمية الـ toxic
+
+448
+00:36:31,050 --> 00:36:35,190
+form of أكسجين فبالتالي البكتيريا هتموت لكن في الـ
+
+449
+00:36:35,190 --> 00:36:38,450
+anaerobic bacteria و الـ facultative anaerobic
+
+450
+00:36:38,450 --> 00:36:42,350
+bacteria الـ toxic form of أكسجين بيتم إزالة
+
+451
+00:36:42,350 --> 00:36:45,980
+السمية عن طريق الإنزيماتالتالي ال super oxide
+
+452
+00:36:45,980 --> 00:36:51,040
+ديسميوتاز الكتلاز والبروكسداز فبالتالي بيتكون non
+
+453
+00:36:51,040 --> 00:36:55,800
+-toxic form اللي هي ال water و ال oxygen البكتيريا
+
+454
+00:36:55,800 --> 00:37:01,520
+بتقدر تعيش طيب كيف أنا بدي أنمي البكتيريا اللي لا
+
+455
+00:37:01,520 --> 00:37:06,640
+هوائية أول طريقة عندي اللي هي ال fluid
+
+456
+00:37:06,640 --> 00:37:11,760
+thioglycolate broth بقولي ال fluid thioglycolate
+
+457
+00:37:11,760 --> 00:37:16,230
+broth is a reducing mediareducing media ليش
+
+458
+00:37:16,230 --> 00:37:20,290
+reducing media بقول لي لأنه بتحتوي على ال Sodium
+
+459
+00:37:20,290 --> 00:37:25,870
+Thioglycolate هذا Sodium Thioglycolate أحد مكونات
+
+460
+00:37:25,870 --> 00:37:30,350
+ال media ال fluid Thioglycolate براسل شو وظيفته
+
+461
+00:37:30,350 --> 00:37:35,380
+removes O2 from the media؟بيزيل ال oxygen الموجود
+
+462
+00:37:35,380 --> 00:37:38,780
+في ال media فبالتالي هيك بقدر أنمي البكتيريا
+
+463
+00:37:38,780 --> 00:37:43,060
+لهوائية يبقى عرفنا ليش هي تعتبر reducing media
+
+464
+00:37:43,060 --> 00:37:46,580
+لأنها بتحتوي على ال sodium-thiozoloycolate اللي
+
+465
+00:37:46,580 --> 00:37:50,280
+بيزيل ال oxygen من ال media فبالتالي بوفر ظروف
+
+466
+00:37:50,280 --> 00:37:54,500
+لهوائية هذه ال media بتدعم ال aerobic and
+
+467
+00:37:54,500 --> 00:37:59,410
+anaerobic بكتيريابقول لي في هاي ال media فيه كاشف
+
+468
+00:37:59,410 --> 00:38:03,890
+هذا الكاشف عشان أعرف هل تم التخلص من ال oxygen ولا
+
+469
+00:38:03,890 --> 00:38:12,710
+لأ Rezazarin is an oxidation reduction indicator
+
+470
+00:38:12,710 --> 00:38:16,650
+that turned to pink in the presence of O2 هذا ال
+
+471
+00:38:16,650 --> 00:38:21,270
+indicator بتحول للون ال pink في حال وجود ال oxygen
+
+472
+00:38:21,270 --> 00:38:26,530
+يبقى في حال وجود ال oxygen بتحول للونالبنك هيك
+
+473
+00:38:26,530 --> 00:38:33,030
+بعرف انه والله انه ال oxygen dial موجود في هاي ال
+
+474
+00:38:33,030 --> 00:38:37,610
+media لم يتم التخلص منه بقولي small amount of agar
+
+475
+00:38:37,610 --> 00:38:41,690
+في عندى كمية قليلة موجودة في ال media to retard
+
+476
+00:38:41,690 --> 00:38:47,300
+gasesليش موجود في عندي نسبة أجار أو أجار بنسبة
+
+477
+00:38:47,300 --> 00:38:53,640
+قليلة جدا لأن الأجار بيعمل Trapping بيحجز البخار
+
+478
+00:38:53,640 --> 00:38:59,420
+وبيحجز الغازات والأكسوجين فبالتالي بيمنع انتشارها
+
+479
+00:38:59,420 --> 00:39:04,640
+في Lithium وبيوفر هيك ظروف لا هوائية فيها المعلومة
+
+480
+00:39:05,000 --> 00:39:09,560
+إنه أنا الـ Thioglycolate breath بروح أنا بسخّنها
+
+481
+00:39:09,560 --> 00:39:13,340
+عشان أطرد أي غازات موجودة في الـ medium إحنا قلنا
+
+482
+00:39:13,340 --> 00:39:18,520
+قبل شوية في كيف أنا بدي أوفر ظروف أو أشوف كيف
+
+483
+00:39:18,520 --> 00:39:22,160
+تصنيفات البكتيريا حسب حاجتها للـ Oxygen قلنا بعد
+
+484
+00:39:22,160 --> 00:39:29,840
+ما أنا أخد اللي هو أحط التيوبات في ال water bath
+
+485
+00:39:29,840 --> 00:39:31,900
+لما أتأكد إنه
+
+486
+00:39:37,270 --> 00:39:42,710
+عشان اضمن ان كل الغازات اللى موجودة في ال media تم
+
+487
+00:39:42,710 --> 00:39:47,310
+طردها هكذا انا وفر الظروف لهوائية هذا بالنسبة ل
+
+488
+00:39:47,740 --> 00:39:52,660
+الأول طريقة لتنمية البكتيريا اللاهوائية لـ Fluid
+
+489
+00:39:52,660 --> 00:39:56,400
+Thioglycolate Broth هي Reducing Media وقلنا ليش
+
+490
+00:39:56,400 --> 00:39:59,720
+Reducing Media؟ شو الـ Indicator الموجود في هذه
+
+491
+00:39:59,720 --> 00:40:05,300
+الـ Media؟ طبعا موجود أكيد نسبة أجار قليلة عشان
+
+492
+00:40:05,300 --> 00:40:09,300
+تعمل Trapping للغازات والأكسجين وأفهر ظروف
+
+493
+00:40:09,300 --> 00:40:14,990
+لاهوائيةتاني طريقة عشان انا اوفر ظروف لاهوائية الـ
+
+494
+00:40:14,990 --> 00:40:20,190
+gas bag system الـ anaerobic jar anaerobic jar
+
+495
+00:40:20,190 --> 00:40:24,150
+such as the gas bag are visible in which an
+
+496
+00:40:24,150 --> 00:40:28,310
+anaerobic environment بقوللي هو عشان اوفر ظروف
+
+497
+00:40:28,310 --> 00:40:32,490
+لاهوائية هذه الظروف اللاهوائية كيف انا بوفرها من
+
+498
+00:40:32,490 --> 00:40:37,010
+خلال is generated after inoculate media are sealed
+
+499
+00:40:37,010 --> 00:40:43,270
+into the chamberبقول لي ان الان european jar هو
+
+500
+00:40:43,270 --> 00:40:48,410
+عبارة عن مرتبان محكم الإغلاق sealed يعني محكم
+
+501
+00:40:48,410 --> 00:40:55,360
+الإغلاقبجيب البكتيريا اللى انا نمتها او اللى انا
+
+502
+00:40:55,360 --> 00:40:59,700
+بالاول زرعتها طبعا زرعت بكتيريا على ميديا high
+
+503
+00:40:59,700 --> 00:41:04,000
+البكتيريا بتحتاج بروفلا هوائية زرعتها على اطباق
+
+504
+00:41:04,000 --> 00:41:08,880
+بروح بحط الانيكلاتي ميديا الميديا المزروعة في هذا
+
+505
+00:41:08,880 --> 00:41:14,700
+ال chamber و بسكره طبعا قبل ما انا اسكره بقوللي في
+
+506
+00:41:14,700 --> 00:41:21,360
+في هذا الجار يكوناللي هو الـ Gas Generator
+
+507
+00:41:21,360 --> 00:41:26,600
+Envelope إيش هذا الـ Gas Generator Envelope عبارة
+
+508
+00:41:26,600 --> 00:41:32,800
+عن كيس هذا الكيس موجود فيه مواد كيميائية تمام يبقى
+
+509
+00:41:32,800 --> 00:41:36,280
+موجود عبارة اللي بالنسبة لألان الـ Aerobic Jar
+
+510
+00:41:36,280 --> 00:41:41,180
+عبارة عن مرتبان بروح أنا بحط الأطباق اللي أنا زرعت
+
+511
+00:41:41,180 --> 00:41:44,980
+البكتيريا اللي بتحتاج في ظروف لاهوائية بحطها
+
+512
+00:41:44,980 --> 00:41:49,880
+upside down في هذا الجرعلى جانب بحط كيس اللى هو ال
+
+513
+00:41:49,880 --> 00:41:54,960
+gas generator generator envelope هذا الكيس موجود
+
+514
+00:41:54,960 --> 00:42:00,700
+فيه مواد كيميائية بروح بضيف عليه water تمام بضيف
+
+515
+00:42:00,700 --> 00:42:03,800
+عليه ميه قبل ما انا طبعا انا اول اشي بفتح الكيس
+
+516
+00:42:03,800 --> 00:42:09,100
+بضيف عليه ميه بكون حتى الأطباق upside down و بعدين
+
+517
+00:42:09,100 --> 00:42:13,880
+بروح بسكر اللى هو هذا المرتبان بيكون لازم يكون
+
+518
+00:42:13,880 --> 00:42:19,900
+محكم الإغلاق بقول ليالـ chemicals طبعا مكملها اللي
+
+519
+00:42:19,900 --> 00:42:23,060
+هو ال gas generator envelope that are placed in
+
+520
+00:42:23,060 --> 00:42:28,320
+the jar just prior to sailing طبعا احنا بنحطه قبل
+
+521
+00:42:28,320 --> 00:42:33,040
+ما انا اسكر المرتبان روحت سكرت المرتبان بقولي ال
+
+522
+00:42:33,040 --> 00:42:35,920
+chemicals in the envelope قولنا في مواد كيميائية
+
+523
+00:42:35,920 --> 00:42:41,020
+في هذا الكيس بقولي بتنتج انا ضفت ال water طبعا
+
+524
+00:42:41,020 --> 00:42:47,540
+فتحته ضفت ال water عشان يحفظ التفاعلبقول لي المواد
+
+525
+00:42:47,540 --> 00:42:50,920
+الكيميائية بيصير في اندي تفاعل كيميائي بينتج
+
+526
+00:42:50,920 --> 00:42:55,600
+hydrogen gas and carbon dioxide بعد ما انا سكرت ال
+
+527
+00:42:55,600 --> 00:43:00,220
+chamber هذا المرتبان صار تفاعل نواتج التفاعل ال
+
+528
+00:43:00,220 --> 00:43:03,740
+hydrogen gas و ال carbon dioxide بقوللي ال
+
+529
+00:43:03,740 --> 00:43:08,620
+hydrogen gas اللي نتج من التفاعل بيرتبط مع ال free
+
+530
+00:43:08,620 --> 00:43:13,460
+oxygen الموجود في ال chamberيبقى الـ hydrogen gas
+
+531
+00:43:13,460 --> 00:43:16,260
+اللى ناتج من التفاعل بيرتبط مع ال oxygen اللى
+
+532
+00:43:16,260 --> 00:43:19,800
+موجود في ال chamber هيك أنا اتخلصت من ال oxygen
+
+533
+00:43:19,800 --> 00:43:24,040
+اللى موجود في ال chamber هينتج في النهاية hydrogen
+
+534
+00:43:24,040 --> 00:43:30,760
+gas مع ال oxygen هينتج مع اندي water يبقى بلاحظ
+
+535
+00:43:30,760 --> 00:43:34,560
+انه او عشان استدل انه صار في اندي ظروف لا هوائية
+
+536
+00:43:34,560 --> 00:43:39,300
+من خلال تعرق الجدار لأنه بتكون عندى waterبقول الـ
+
+537
+00:43:39,300 --> 00:43:43,640
+carbon الـ carbon dioxide اللي نتج من التفاعل is
+
+538
+00:43:43,640 --> 00:43:46,480
+required for the growth of certain organisms في
+
+539
+00:43:46,480 --> 00:43:51,600
+بعض أنواع البكتيريا بتحتاج الـ CO2 لانمو مثليني
+
+540
+00:43:51,600 --> 00:43:55,000
+blue indicator strip في عندي أنا strip مثليني blue
+
+541
+00:43:55,000 --> 00:44:00,160
+indicator strip موجودة في هذا الجار usually placed
+
+542
+00:44:00,160 --> 00:44:03,620
+in the jar it turned colorless when the oxygen has
+
+543
+00:44:03,620 --> 00:44:08,470
+been removedفي هذا الطريقة في عندي الـ methylene
+
+544
+00:44:08,470 --> 00:44:13,170
+blue indicator strip بقول لي بيصير لونها colorless
+
+545
+00:44:13,170 --> 00:44:17,930
+لو الأكسجين تم إزالته عشان أتأكد أنه الأكسجين تم
+
+546
+00:44:17,930 --> 00:44:22,170
+إزالته وتوفر عندي ظروف لا هوائية بيصير هذا ال
+
+547
+00:44:22,170 --> 00:44:26,730
+strip لونه colorless هذا بالنسبة للـ anaerobic jar
+
+548
+00:44:26,730 --> 00:44:32,810
+هنشوفه في هاي ال slide هاي ال slide هو موضح ال
+
+549
+00:44:32,810 --> 00:44:38,470
+anaerobic jarبقول لي او اذا احنا ملاحظين في ال هذا
+
+550
+00:44:38,470 --> 00:44:43,550
+ال هاي ال slide في عندي gas generator envelope في
+
+551
+00:44:43,550 --> 00:44:47,910
+عندي case هذا ال case قولنا فيه مواد كيميائية انا
+
+552
+00:44:47,910 --> 00:44:52,610
+اول شئ بصفط الأطباق upside down تمام اللي انا
+
+553
+00:44:52,610 --> 00:44:56,370
+زراعت فيها البكتيريا بتحتاج ظروف لاهوائيةموجود هذا
+
+554
+00:44:56,370 --> 00:44:59,290
+الكيس الموجود فيه المواد الكيميائية بفتح هذا الكيس
+
+555
+00:44:59,290 --> 00:45:04,550
+بضيف ميه water is added to chemical in envelope
+
+556
+00:45:04,550 --> 00:45:11,670
+هينتج عندي ال H2 وCO2 من هذا ال gas او من هذا ال
+
+557
+00:45:11,670 --> 00:45:17,750
+envelope تمام بقوللي انه ال hydrogen gas هيرتبط مع
+
+558
+00:45:17,750 --> 00:45:21,290
+ال oxygen الموجود في ال chamber هيتكون عندي water
+
+559
+00:45:21,290 --> 00:45:25,050
+فبالتالي هيك انا قدرت اتخلص من ال oxygen الموجود
+
+560
+00:45:25,050 --> 00:45:30,400
+فيلتشامبر هيك انا وفرت ظروف لا هوائية ال carbon ال
+
+561
+00:45:30,400 --> 00:45:35,180
+carbon dioxide بقول ليه بيلزم ل growth of certain
+
+562
+00:45:35,180 --> 00:45:39,540
+microorganism هان في عندي قولنا الان ال indicator
+
+563
+00:45:39,540 --> 00:45:43,960
+strip اللي هي ال methylene blue في حال توفر عندي
+
+564
+00:45:43,960 --> 00:45:47,420
+ظروف لا هوائية في حال نقص ال oxygen or absence of
+
+565
+00:45:47,420 --> 00:45:52,530
+oxygen بيصير لونها colorlessOxygen is removed from
+
+566
+00:45:52,530 --> 00:45:55,110
+the chamber الـ Oxygen بيتم إزالته من ال chamber
+
+567
+00:45:55,110 --> 00:45:59,330
+من خلال combining with hydrogen هيرتبط مع ال
+
+568
+00:45:59,330 --> 00:46:04,750
+hydrogen اللي ناتج من التفاعل من اللي هو ال gas in
+
+569
+00:46:04,750 --> 00:46:08,390
+generator envelope to form water هيتكون عندي
+
+570
+00:46:08,390 --> 00:46:12,030
+بالنهاية water قولنا باستدل على التفاعل من خلال
+
+571
+00:46:12,030 --> 00:46:16,830
+تعرق الجدار لأنه اتكون عندي waterولهذا التفاعل
+
+572
+00:46:16,830 --> 00:46:20,210
+this reaction اللي هو ارتباط الـ H الهيدروجين جاز
+
+573
+00:46:20,210 --> 00:46:25,230
+مع الـ O2 catalyzed by the palladium pellets بيتم
+
+574
+00:46:25,230 --> 00:46:28,490
+تحفيزه باستخدام ال palladium pellets اللي بتكون
+
+575
+00:46:28,490 --> 00:46:33,250
+موجودة في ال chamber هذا بالنسبة للآن يورو بيك جار
+
+576
+00:46:33,250 --> 00:46:38,390
+طب في عندي نفس الفكرة لكن مش جار بيكون gas bag
+
+577
+00:46:38,390 --> 00:46:44,640
+powerاللي هو Case مش مرتبان بدل المرتبان بيكون
+
+578
+00:46:44,640 --> 00:46:49,420
+موجود Case نفس الفكرة بيكون في Case داخله مواد
+
+579
+00:46:49,420 --> 00:46:53,320
+كيميائية بروح أنا بفتح ال Case بضيف عليه الميه
+
+580
+00:46:53,320 --> 00:47:00,100
+بيصير تفاعل بوجود محفظ معين بيحفظ التفاعل بيرتبط
+
+581
+00:47:00,100 --> 00:47:04,140
+بالنهاية بينتج اللي هو بينتج نفس الفكرة اللي كانت
+
+582
+00:47:04,140 --> 00:47:08,770
+في الجعار بينتج H2هيرتبط مع ال oxygen اللى موجود
+
+583
+00:47:08,770 --> 00:47:13,430
+داخل هذا ال case هك بيوفر ظروف لا هوائية طبعا انا
+
+584
+00:47:13,430 --> 00:47:17,470
+بحط الأطباق في هذا ال case طبعا مش مش زى ال jar
+
+585
+00:47:17,470 --> 00:47:21,430
+بحط الأطباق فى ال case بقولك هان اخر خطوة انك انت
+
+586
+00:47:21,430 --> 00:47:28,500
+لازمطبعا تستخدمي شريط عشان او مثلا ملقط تسكري هذا
+
+587
+00:47:28,500 --> 00:47:32,620
+الكيس عشان يوفر الظروف اللي هوائية بعدين بتاخدي
+
+588
+00:47:32,620 --> 00:47:36,260
+هذا الكيس و بتحطيه في ال incubation هيك انا بوفر
+
+589
+00:47:36,260 --> 00:47:41,020
+ظروف لا هوائية نفس فكرة المرتبان لكن هو عبارة عن
+
+590
+00:47:41,020 --> 00:47:45,100
+كيس بحط الأطباق داخل الكيس و بسكر هذا الكيس
+
+591
+00:47:45,100 --> 00:47:49,360
+باستخدام sealing bar اللي هو شريط او ملقط لكن لازم
+
+592
+00:47:49,360 --> 00:47:54,450
+يكون اتأكد ان هو محكم الإغلاقهذا بالنسبة للجاز باج
+
+593
+00:47:54,450 --> 00:48:00,950
+باش اللي هو ال case نيجي ل ال candle jar ال candle
+
+594
+00:48:00,950 --> 00:48:04,750
+jar ال candle jar is used to create micro
+
+595
+00:48:04,750 --> 00:48:09,490
+aerophilic condition هذا ال candle jar يستخدم
+
+596
+00:48:09,490 --> 00:48:13,710
+لتوفير الظروف اللي هي البكتيريا أو لتنمية
+
+597
+00:48:13,710 --> 00:48:18,120
+البكتيرياالـ Micro Aerophilic Bacteria الـ Micro
+
+598
+00:48:18,120 --> 00:48:22,360
+Aerophilic Bacteria قلنا بتحتاج كمية قليلة من
+
+599
+00:48:22,360 --> 00:48:27,060
+الأكسجين للنمو شوف فكرة ال candle jar هو عبارة عن
+
+600
+00:48:27,060 --> 00:48:33,370
+مرتبان محكم الإغلاق تمام محكم الإغلاقبحط فيه اللي
+
+601
+00:48:33,370 --> 00:48:38,110
+هي ال medium البكتيريا اللي انا او الأطباق
+
+602
+00:48:38,110 --> 00:48:43,230
+البكتيريا اللي انا زرعتها على ال media على الطبق
+
+603
+00:48:43,230 --> 00:48:47,930
+اللي بتحتاج ظروف او بتحتاج جميعة قليلة من ال
+
+604
+00:48:47,930 --> 00:48:52,850
+oxygen بحطها upside down بقوللي اخر شئ بتحطي ال
+
+605
+00:48:52,850 --> 00:48:57,490
+candle بتشعلي ال candle اللي هي الشمعة ممكن احنا
+
+606
+00:48:57,490 --> 00:49:03,470
+نجيب slideبنثبتها على آخر اللي هو petri dish و
+
+607
+00:49:03,470 --> 00:49:09,070
+بشغل الشمعة بولّع الشمعة تمام و بروح أنا بسكر هذا
+
+608
+00:49:09,070 --> 00:49:12,990
+ال candle أو بسكر ال jar ال candle ال jar بسكره
+
+609
+00:49:12,990 --> 00:49:17,990
+بعد ما أنا اولّع الشمعة تمام ال candle is lit بضو
+
+610
+00:49:17,990 --> 00:49:21,190
+الشمعة and the jar is sealed و بسكر اللي هو
+
+611
+00:49:21,190 --> 00:49:25,310
+المرتبان بقول لي ال candle will burn and reduce
+
+612
+00:49:25,310 --> 00:49:30,240
+the oxygen concentration هان انهبعد ما انت تولعي
+
+613
+00:49:30,240 --> 00:49:36,420
+الشمعة وتسكري المرتبان الشمعة هتحرق كل ال Oxygen
+
+614
+00:49:36,420 --> 00:49:44,420
+الموجود في داخل الجار فبالتالي هيطلع CO2 لازم
+
+615
+00:49:44,420 --> 00:49:48,120
+لنموء البكتيريا رح يظل نسبة قليلة من ال Oxygen هيك
+
+616
+00:49:48,120 --> 00:49:52,800
+انا وفرت ظروف مناسبة لـMicro Aerophilic Bacteria
+
+617
+00:49:54,180 --> 00:49:57,700
+طبعا اسم اللى هو الطريقة ال candle jar لإني أنا
+
+618
+00:49:57,700 --> 00:50:03,400
+بستخدم candle شمعة فيهاي الطريقة فترة الحضانة طبعا
+
+619
+00:50:03,400 --> 00:50:09,700
+لا تقل عن 48 ساعة حتى تنمو البكتيريا و تتكثر
+
+620
+00:50:09,700 --> 00:50:15,140
+بالنسبة لأخر شئ اللى هو ال CO2 generating packet
+
+621
+00:50:19,700 --> 00:50:23,140
+طبعا هذا عشان اوفر ظروف الـ CO2 بعض أنواع
+
+622
+00:50:23,140 --> 00:50:27,820
+البكتيريا قلنا بتحتاج للـ CO2 لانمو نفس الفكرة في
+
+623
+00:50:27,820 --> 00:50:32,100
+بكون عندي envelope تمام بيوجد داخله gas generator
+
+624
+00:50:32,100 --> 00:50:37,940
+عشان اللي هو يكون عندي الجاز بحط ال culture
+
+625
+00:50:37,940 --> 00:50:41,340
+البكتيريا في هذا ال case طبعا هان Hyperbolic
+
+626
+00:50:41,340 --> 00:50:41,640
+Hyperbolic Hyperbolic Hyperbolic Hyperbolic
+
+627
+00:50:41,640 --> 00:50:42,000
+Hyperbolic Hyperbolic Hyperbolic Hyperbolic
+
+628
+00:50:42,000 --> 00:50:43,180
+Hyperbolic Hyperbolic Hyperbolic Hyperbolic
+
+629
+00:50:43,180 --> 00:50:43,560
+Hyperbolic Hyperbolic Hyperbolic Hyperbolic
+
+630
+00:50:43,560 --> 00:50:43,780
+Hyperbolic Hyperbolic Hyperbolic Hyperbolic
+
+631
+00:50:43,780 --> 00:50:43,840
+Hyperbolic Hyperbolic Hyperbolic Hyperbolic
+
+632
+00:50:43,840 --> 00:50:47,890
+Hyperbolic Hyperbolic Hyperbهكذا نتخلص من الـ O2
+
+633
+00:50:47,890 --> 00:50:52,950
+ونحصل على الـ CO2 اللازم للنمو من خلال الـ Gas
+
+634
+00:50:52,950 --> 00:50:59,790
+Generator طبعا هذا يختص بالبكتيريا الهوائية اخر
+
+635
+00:50:59,790 --> 00:51:02,790
+اشي في هذا ال chapter اللي هو الـ anaerobic
+
+636
+00:51:02,790 --> 00:51:09,470
+chamber بلي هذا ال anaerobic chamber provide
+
+637
+00:51:09,470 --> 00:51:15,640
+a strict anaerobic atmosphericعشان انا اوفر ظروف
+
+638
+00:51:15,640 --> 00:51:21,300
+لهوائية طبعا باستخدام اللي هو palladium catalyst
+
+639
+00:51:21,300 --> 00:51:27,060
+and hydrogen gas mixed of 5%طبعا في عندي بيكون
+
+640
+00:51:27,060 --> 00:51:34,080
+اللي هو chamber او زي container او chamber كبير
+
+641
+00:51:34,080 --> 00:51:38,100
+اذا ما نلاحظ في هذه الصورة كل الإدوات موجودة داخل
+
+642
+00:51:38,100 --> 00:51:43,740
+هذا ال chamber بدخل فقط او اني انا بشتغل من خلال
+
+643
+00:51:43,740 --> 00:51:46,360
+قفازات متطيئة زي ما ملاحظين في هذه الصورة في عندي
+
+644
+00:51:46,360 --> 00:51:50,580
+أنا قفازات من خلال هذه القفازات انا بقدر اشتغل
+
+645
+00:51:50,580 --> 00:51:55,350
+داخل هذا ال chamberهك بيوفر طبعا او بمنع دخول ال
+
+646
+00:51:55,350 --> 00:51:59,710
+oxygen لداخل ال chamber بالنسبة للظروف اللي
+
+647
+00:51:59,710 --> 00:52:04,130
+لاهوائية كيف بتتكون طبعا في انديهيدروجين جاز بيصير
+
+648
+00:52:04,130 --> 00:52:07,190
+تفاعل بين ال hydrogen gas و ال oxygen اللي موجود
+
+649
+00:52:07,190 --> 00:52:12,010
+داخل ال chamber هك بتخلص من اللي هو ال oxygen بوفر
+
+650
+00:52:12,010 --> 00:52:16,410
+ظروف CO2 بالتالي بتتوفر الظروف اللي لاهوائية
+
+651
+00:52:17,720 --> 00:52:26,880
+الباليديوم كان مهم كمحفز لإرتباط الـH2 مع الأكسجين
+
+652
+00:52:26,880 --> 00:52:31,740
+عشان أتخلص من الأكسجين والذي يتكون عندى الـH2O
+
+653
+00:52:31,740 --> 00:52:37,740
+هكذا نكون خلصنا الـchamber يعطيكم العافية والسلام
+
+654
+00:52:37,740 --> 00:52:39,680
+عليكم ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/Pl7CGd4BP8U_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/Pl7CGd4BP8U_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..9b2d59d9ca37cb731ee07bc8b8f8bccc83696e33
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/Pl7CGd4BP8U_raw.json
@@ -0,0 +1 @@
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{"id": 13, "seek": 34522, "start": 318.28, "end": 345.22, "text": "طبعاً الـ Oxygen في الـ Air نسبة الـ Oxygen في الهواء 21% إذا كان نسبة الـ Oxygen أعلى موجودة في الـ media أو في الهواء عامة بتحتاجه البكتيريا يعتبر toxic فبالتالي في هذه الحالتين لو البكتيريا ما عندها انزيمات لاتخلص من الـ Oxygen أو من الـ toxic form of Oxygen", "tokens": [9566, 3555, 3615, 995, 14111, 2423, 39184, 16489, 8647, 8978, 2423, 39184, 5774, 8717, 35457, 3660, 2423, 39184, 16489, 8647, 8978, 2423, 3224, 2407, 16606, 5080, 4, 11933, 15730, 25961, 8717, 35457, 3660, 2423, 39184, 16489, 8647, 5551, 3615, 23942, 3714, 29245, 23328, 3660, 8978, 2423, 39184, 3021, 34051, 8978, 2423, 3224, 2407, 16606, 6225, 10943, 3660, 39894, 33753, 26108, 3224, 29739, 4117, 2655, 13546, 25528, 7251, 34268, 26890, 12786, 6156, 3555, 6027, 2655, 6027, 1829, 8978, 29538, 21542, 6027, 2655, 9957, 45164, 29739, 4117, 2655, 13546, 25528, 19446, 43242, 11296, 16472, 11622, 32640, 9307, 5296, 9307, 9778, 1211, 9381, 9154, 2423, 39184, 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" الـ", "probability": 0.88232421875}, {"start": 343.7, "end": 344.0, "word": " toxic", "probability": 0.62841796875}, {"start": 344.0, "end": 344.46, "word": " form", "probability": 0.93359375}, {"start": 344.46, "end": 344.68, "word": " of", "probability": 0.943359375}, {"start": 344.68, "end": 345.22, "word": " Oxygen", "probability": 0.943115234375}], "temperature": 1.0}, {"id": 14, "seek": 36777, "start": 345.65, "end": 367.77, "text": "أو لو كان نسبة الأكسجين في الميديا أو في الهواء أعلى من حاجة أو أعلى من الكمية اللى بتحتاجها البكتيريا هيكون toxic في هذه الحالات أكيد البكتيريا هتلجأ للظروف اللاهوائية للحصول على الطاقة طيب نيجي لاقى ال toxic form of oxygen", "tokens": [10721, 2407, 45164, 25961, 8717, 35457, 3660, 16247, 4117, 3794, 7435, 9957, 8978, 9673, 1829, 16254, 995, 34051, 8978, 2423, 3224, 2407, 16606, 5551, 3615, 23942, 9154, 11331, 26108, 3660, 34051, 5551, 3615, 23942, 9154, 33251, 2304, 10632, 13672, 7578, 39894, 33753, 26108, 11296, 29739, 4117, 2655, 13546, 25528, 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single oxygen بقول لي is in high energy state extremely reactive exist in phagocyte بقولي هذا هو يعتبر high energy state بيكون extremely reactive نشط بتستخدمه بيتم استخدامه في phagocytosis في البلعمة", "tokens": [10721, 12610, 1254, 5296, 10721, 12610, 13412, 28820, 9154, 5551, 8592, 4117, 6027, 2423, 9169, 13672, 1829, 39894, 30544, 12786, 2167, 9169, 4724, 39648, 32239, 307, 294, 1090, 2281, 1785, 4664, 28897, 2514, 294, 903, 6442, 1344, 975, 4724, 39648, 1829, 23758, 31439, 7251, 34268, 26890, 1090, 2281, 1785, 4724, 1829, 30544, 4664, 28897, 8717, 8592, 9566, 39894, 14851, 9778, 40448, 3224, 4724, 36081, 2304, 44713, 9778, 3215, 10943, 3224, 8978, 903, 6442, 1344, 83, 8211, 8978, 29739, 1211, 25957, 3660], "avg_logprob": -0.1861661598086357, "compression_ratio": 1.6084905660377358, "no_speech_prob": 2.384185791015625e-07, "words": [{"start": 368.53, "end": 369.17, "word": "أول", "probability": 0.978759765625}, {"start": 369.17, "end": 370.25, "word": " form", "probability": 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used as antimicrobial agent ايش يعني antimicrobial agent يعني مادة تستخدم في قتل أو تثبيت البكتيري هذا معناه ال antimicrobial agent اللي هنتكلم عنه بالتفصيل في آخر chapter في المساق", "tokens": [3224, 15730, 31439, 1687, 28421, 1737, 12001, 4032, 1975, 4117, 2655, 35457, 9122, 2304, 7649, 6084, 19753, 34740, 1143, 382, 46141, 299, 16614, 831, 9461, 1975, 1829, 8592, 37495, 22653, 46141, 299, 16614, 831, 9461, 37495, 22653, 3714, 18513, 3660, 6055, 14851, 9778, 40448, 8978, 12174, 2655, 1211, 34051, 6055, 12984, 21292, 2655, 29739, 4117, 2655, 13546, 1829, 23758, 20449, 8315, 3224, 2423, 46141, 299, 16614, 831, 9461, 13672, 1829, 8032, 29399, 4117, 19528, 18871, 3224, 20666, 2655, 5172, 9381, 26895, 8978, 19753, 34740, 7187, 8978, 9673, 3794, 995, 4587], "avg_logprob": -0.17419299909046718, "compression_ratio": 1.5833333333333333, "no_speech_prob": 1.430511474609375e-06, "words": [{"start": 492.12, "end": 492.56, "word": "هذا", "probability": 0.86572265625}, {"start": 492.56, "end": 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لا هوائية هيكون في كل tube أكتر إش هيكون في الأسفل لأن هي لا هوائية لكن ممكن أني أنا ألاقيها في الأعلى لأنه بتتحمل كمية معينة من ال oxygen", "tokens": [3224, 15040, 5016, 19913, 14739, 3224, 20666, 1863, 35457, 3660, 24976, 39184, 32459, 310, 27035, 394, 293, 11207, 22234, 11763, 8032, 2655, 30544, 23032, 3555, 3615, 995, 13672, 1829, 31439, 8978, 3224, 8717, 2304, 2407, 11296, 39896, 30544, 39896, 23032, 3555, 3615, 995, 20193, 8032, 2407, 16373, 10632, 39896, 30544, 8978, 28242, 9917, 5551, 4117, 2655, 2288, 11933, 8592, 39896, 30544, 8978, 16247, 3794, 5172, 1211, 5296, 33456, 39896, 20193, 8032, 2407, 16373, 10632, 44381, 3714, 43020, 14739, 1829, 41850, 5551, 15040, 38436, 11296, 8978, 16247, 3615, 23942, 5296, 33456, 3224, 39894, 2655, 35571, 1211, 9122, 2304, 10632, 20449, 9957, 3660, 9154, 2423, 9169], "avg_logprob": -0.158928572563898, "compression_ratio": 1.6986899563318778, "no_speech_prob": 4.4226646423339844e-05, "words": [{"start": 1636.51, "end": 1637.67, "word": 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{"start": 2232.48, "end": 2232.74, "word": " a", "probability": 0.9677734375}, {"start": 2232.74, "end": 2233.28, "word": " reducing", "probability": 0.89990234375}, {"start": 2233.28, "end": 2234.1, "word": " media", "probability": 0.55615234375}], "temperature": 1.0}, {"id": 86, "seek": 225277, "start": 2234.67, "end": 2252.77, "text": "reducing media ليش reducing media بقول لي لأنه بتحتوي على ال Sodium Thioglycolate هذا Sodium Thioglycolate أحد مكونات ال media ال fluid Thioglycolate براسل شو وظيفته removes O2 from the media؟", "tokens": [265, 769, 2175, 3021, 32239, 8592, 12245, 3021, 4724, 39648, 32239, 5296, 33456, 3224, 39894, 33753, 45865, 15844, 2423, 42059, 2197, 334, 1004, 70, 356, 8768, 473, 23758, 42059, 2197, 334, 1004, 70, 356, 8768, 473, 5551, 24401, 3714, 4117, 2407, 8315, 2655, 2423, 3021, 2423, 9113, 334, 1004, 70, 356, 8768, 473, 4724, 23557, 3794, 1211, 13412, 2407, 4032, 19913, 33911, 47395, 30445, 422, 17, 490, 264, 3021, 22807], "avg_logprob": 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بيزيل ال oxygen من ال media فبالتالي بوفر ظروف لهوائية هذه ال media بتدعم ال aerobic and anaerobic بكتيريا", "tokens": [21292, 11622, 26895, 2423, 9169, 9673, 29245, 23328, 8978, 2423, 3021, 6156, 3555, 6027, 2655, 6027, 1829, 39896, 4117, 4724, 28543, 2288, 14739, 2304, 1829, 29739, 4117, 2655, 13546, 25528, 46740, 2407, 16373, 10632, 7251, 3555, 4587, 7578, 6225, 28480, 8315, 32239, 8592, 39896, 6055, 34268, 26890, 12245, 3021, 5296, 33456, 11296, 39894, 33753, 45865, 15844, 2423, 20265, 12, 392, 1004, 89, 401, 939, 8768, 473, 13672, 1829, 4724, 1829, 11622, 26895, 2423, 9169, 9154, 2423, 3021, 6156, 3555, 6027, 2655, 6027, 1829, 4724, 38688, 2288, 1357, 116, 32887, 5172, 46740, 2407, 16373, 10632, 29538, 2423, 3021, 39894, 3215, 25957, 2423, 11207, 22234, 293, 34178, 260, 22234, 4724, 4117, 2655, 13546, 25528], "avg_logprob": -0.17228982300884957, "compression_ratio": 1.7887931034482758, "no_speech_prob": 1.138448715209961e-05, "words": [{"start": 2253.3, "end": 2254.16, "word": 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أعرف هل تم التخلص من ال oxygen ولا لأ Rezazarin is an oxidation reduction indicator that turned to pink in the presence of O2 هذا ال indicator بتحول للون ال pink في حال وجود ال oxygen يبقى في حال وجود ال oxygen بتحول للون", "tokens": [3555, 39648, 32239, 8978, 8032, 47302, 2423, 3021, 8978, 3224, 9122, 33599, 5172, 23758, 33251, 33599, 5172, 6225, 8592, 7649, 5551, 3615, 28480, 8032, 1211, 46811, 16712, 9778, 1211, 9381, 9154, 2423, 9169, 49429, 5296, 10721, 1300, 89, 921, 19829, 307, 364, 36767, 11004, 16961, 300, 3574, 281, 7022, 294, 264, 6814, 295, 422, 17, 23758, 2423, 16961, 39894, 5016, 12610, 24976, 11536, 2423, 7022, 8978, 11331, 6027, 49610, 23328, 2423, 9169, 7251, 3555, 4587, 7578, 8978, 11331, 6027, 49610, 23328, 2423, 9169, 39894, 5016, 12610, 24976, 11536], "avg_logprob": -0.20856741573033707, "compression_ratio": 1.721461187214612, "no_speech_prob": 4.172325134277344e-07, "words": [{"start": 2276.47, "end": 2276.87, "word": "بقول", "probability": 0.802001953125}, 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{"start": 2401.1, "end": 2401.5, "word": " طبعا", "probability": 0.948974609375}, {"start": 2401.5, "end": 2401.84, "word": " موجود", "probability": 0.8894856770833334}, {"start": 2401.84, "end": 2402.26, "word": " أكيد", "probability": 0.8623046875}, {"start": 2402.26, "end": 2402.78, "word": " نسبة", "probability": 0.9890950520833334}, {"start": 2402.78, "end": 2403.4, "word": " أجار", "probability": 0.93701171875}, {"start": 2403.4, "end": 2404.48, "word": " قليلة", "probability": 0.9747721354166666}, {"start": 2404.48, "end": 2405.3, "word": " عشان", "probability": 0.9348958333333334}, {"start": 2405.3, "end": 2405.74, "word": " تعمل", "probability": 0.9933268229166666}, {"start": 2405.74, "end": 2406.4, "word": " Trapping", "probability": 0.55645751953125}, {"start": 2406.4, "end": 2407.76, "word": " للغازات", "probability": 0.887939453125}, {"start": 2407.76, "end": 2408.42, "word": " والأكسجين", "probability": 0.8895670572916666}, {"start": 2408.42, "end": 2408.9, "word": " وأفهر", "probability": 0.64862060546875}, {"start": 2408.9, "end": 2409.3, "word": " ظروف", "probability": 0.9493408203125}, {"start": 2409.3, "end": 2410.02, "word": " لاهوائية", "probability": 0.96669921875}], "temperature": 1.0}, {"id": 94, "seek": 243935, "start": 2411.33, "end": 2439.35, "text": "تاني طريقة عشان انا اوفر ظروف لاهوائية الـ gas bag system الـ anaerobic jar anaerobic jar such as the gas bag are visible in which an anaerobic environment بقوللي هو عشان اوفر ظروف لاهوائية هذه الظروف اللاهوائية كيف انا بوفرها من خلال is generated after inoculate media are sealed into the chamber", "tokens": [2655, 7649, 1829, 23032, 16572, 28671, 6225, 8592, 7649, 1975, 8315, 1975, 38688, 2288, 1357, 116, 32887, 5172, 20193, 3224, 2407, 16373, 10632, 2423, 39184, 4211, 3411, 1185, 2423, 39184, 34178, 260, 22234, 15181, 34178, 260, 22234, 15181, 1270, 382, 264, 4211, 3411, 366, 8974, 294, 597, 364, 34178, 260, 22234, 2823, 4724, 39648, 20292, 31439, 6225, 8592, 7649, 1975, 38688, 2288, 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"end": 2421.93, "word": " gas", "probability": 0.89111328125}, {"start": 2421.93, "end": 2422.41, "word": " bag", "probability": 0.69091796875}, {"start": 2422.41, "end": 2422.71, "word": " are", "probability": 0.89697265625}, {"start": 2422.71, "end": 2423.13, "word": " visible", "probability": 0.9130859375}, {"start": 2423.13, "end": 2423.49, "word": " in", "probability": 0.96728515625}, {"start": 2423.49, "end": 2423.79, "word": " which", "probability": 0.927734375}, {"start": 2423.79, "end": 2424.15, "word": " an", "probability": 0.434814453125}, {"start": 2424.15, "end": 2424.83, "word": " anaerobic", "probability": 0.9576822916666666}, {"start": 2424.83, "end": 2425.65, "word": " environment", "probability": 0.93896484375}, {"start": 2425.65, "end": 2426.27, "word": " بقوللي", "probability": 0.5994466145833334}, {"start": 2426.27, "end": 2427.03, "word": " هو", "probability": 0.80615234375}, {"start": 2427.03, "end": 2427.45, "word": " عشان", "probability": 0.9944661458333334}, {"start": 2427.45, "end": 2427.89, "word": " اوفر", "probability": 0.9446614583333334}, {"start": 2427.89, "end": 2428.31, "word": " ظروف", "probability": 0.97021484375}, {"start": 2428.31, "end": 2429.17, "word": " لاهوائية", "probability": 0.975390625}, {"start": 2429.17, "end": 2429.61, "word": " هذه", "probability": 0.6201171875}, {"start": 2429.61, "end": 2429.95, "word": " الظروف", "probability": 0.967041015625}, {"start": 2429.95, "end": 2430.69, "word": " اللاهوائية", "probability": 0.91025390625}, {"start": 2430.69, "end": 2431.25, "word": " كيف", "probability": 0.9501953125}, {"start": 2431.25, "end": 2431.57, "word": " انا", "probability": 0.818115234375}, {"start": 2431.57, "end": 2432.31, "word": " بوفرها", "probability": 0.9886474609375}, {"start": 2432.31, "end": 2432.49, "word": " من", "probability": 0.98779296875}, {"start": 2432.49, "end": 2433.19, "word": " خلال", "probability": 0.9959309895833334}, {"start": 2433.19, "end": 2433.79, "word": " is", "probability": 0.115234375}, {"start": 2433.79, "end": 2434.37, "word": " generated", "probability": 0.8818359375}, {"start": 2434.37, "end": 2435.13, "word": " after", "probability": 0.88818359375}, {"start": 2435.13, "end": 2435.87, "word": " inoculate", "probability": 0.6241048177083334}, {"start": 2435.87, "end": 2436.27, "word": " media", "probability": 0.93115234375}, {"start": 2436.27, "end": 2436.71, "word": " are", "probability": 0.935546875}, {"start": 2436.71, "end": 2437.01, "word": " sealed", "probability": 0.93212890625}, {"start": 2437.01, "end": 2437.29, "word": " into", "probability": 0.73193359375}, {"start": 2437.29, "end": 2438.73, "word": " the", "probability": 0.7734375}, {"start": 2438.73, "end": 2439.35, "word": " chamber", "probability": 0.71044921875}], "temperature": 1.0}, {"id": 95, "seek": 244921, "start": 2440.53, "end": 2449.21, "text": "بقول لي ان الان european jar هو عبارة عن مرتبان محكم الإغلاق sealed يعني محكم الإغلاق", "tokens": [3555, 39648, 32239, 16472, 2423, 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طبعا قبل ما انا اسكره بقوللي في في هذا الجار يكون", "tokens": [3555, 7435, 1829, 3555, 29739, 4117, 2655, 13546, 25528, 13672, 7578, 1975, 8315, 8717, 2304, 2655, 11296, 1975, 2407, 13672, 7578, 1975, 8315, 20666, 995, 12610, 30767, 2288, 34268, 11296, 23032, 3555, 3615, 995, 30767, 2288, 34268, 4724, 4117, 2655, 13546, 25528, 15844, 3714, 1829, 16254, 995, 1090, 29739, 4117, 2655, 13546, 25528, 39894, 33753, 26108, 4724, 2288, 38688, 15040, 8032, 2407, 16373, 10632, 30767, 2288, 34268, 11296, 15844, 1975, 9566, 3555, 995, 4587, 4724, 32887, 5016, 4724, 5016, 9566, 2423, 7649, 1829, 28820, 9307, 1829, 3714, 1829, 16254, 995, 9673, 1829, 16254, 995, 9673, 11622, 32887, 27884, 8978, 23758, 2423, 13610, 4032, 4724, 3794, 37983, 3224, 23032, 3555, 3615, 995, 12174, 36150, 19446, 1975, 8315, 24525, 37983, 3224, 4724, 39648, 20292, 8978, 8978, 23758, 25724, 9640, 7251, 30544], "avg_logprob": -0.21622596853054488, "compression_ratio": 2.141509433962264, "no_speech_prob": 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زرعت", "probability": 0.9059244791666666}, {"start": 2458.08, "end": 2458.82, "word": " بكتيريا", "probability": 0.9787109375}, {"start": 2458.82, "end": 2459.06, "word": " على", "probability": 0.81201171875}, {"start": 2459.06, "end": 2459.42, "word": " ميديا", "probability": 0.81396484375}, {"start": 2459.42, "end": 2459.7, "word": " high", "probability": 0.148193359375}, {"start": 2459.7, "end": 2460.54, "word": " البكتيريا", "probability": 0.9767578125}, {"start": 2460.54, "end": 2461.08, "word": " بتحتاج", "probability": 0.9122721354166666}, {"start": 2461.08, "end": 2461.56, "word": " بروفلا", "probability": 0.534271240234375}, {"start": 2461.56, "end": 2462.14, "word": " هوائية", "probability": 0.8345947265625}, {"start": 2462.14, "end": 2463.24, "word": " زرعتها", "probability": 0.977783203125}, {"start": 2463.24, "end": 2463.44, "word": " على", "probability": 0.94580078125}, {"start": 2463.44, "end": 2464.0, "word": " اطباق", "probability": 0.89169921875}, {"start": 2464.0, 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0.97705078125}, {"start": 2888.63, "end": 2889.49, "word": " يستخدم", "probability": 0.9456787109375}, {"start": 2889.49, "end": 2890.75, "word": " لتوفير", "probability": 0.978515625}, {"start": 2890.75, "end": 2891.35, "word": " الظروف", "probability": 0.9488525390625}, {"start": 2891.35, "end": 2891.51, "word": " اللي", "probability": 0.943603515625}, {"start": 2891.51, "end": 2892.07, "word": " هي", "probability": 0.95068359375}, {"start": 2892.07, "end": 2893.01, "word": " البكتيريا", "probability": 0.9361328125}, {"start": 2893.01, "end": 2893.19, "word": " أو", "probability": 0.544921875}, {"start": 2893.19, "end": 2893.71, "word": " لتنمية", "probability": 0.94111328125}, {"start": 2893.71, "end": 2894.45, "word": " البكتيريا", "probability": 0.98642578125}], "temperature": 1.0}, {"id": 113, "seek": 291178, "start": 2894.84, "end": 2911.78, "text": "الـ Micro Aerophilic Bacteria الـ Micro Aerophilic Bacteria قلنا بتحتاج كمية قليلة من الأكسجين للنمو شوف فكرة ال candle jar هو عبارة عن مرتبان محكم الإغلاق تمام محكم الإغلاق", "tokens": [6027, 39184, 25642, 32459, 5317, 388, 299, 363, 14125, 654, 2423, 39184, 25642, 32459, 5317, 388, 299, 363, 14125, 654, 12174, 1211, 8315, 39894, 33753, 26108, 9122, 2304, 10632, 12174, 20292, 37977, 9154, 16247, 4117, 3794, 7435, 9957, 24976, 1863, 2304, 2407, 13412, 38688, 6156, 4117, 25720, 2423, 17968, 15181, 31439, 6225, 3555, 9640, 3660, 18871, 3714, 43500, 3555, 7649, 3714, 5016, 24793, 33688, 17082, 15040, 4587, 46811, 10943, 3714, 5016, 24793, 33688, 17082, 15040, 4587], "avg_logprob": -0.19632710419692, "compression_ratio": 1.6134969325153374, "no_speech_prob": 6.902217864990234e-05, "words": [{"start": 2894.84, "end": 2895.38, "word": "الـ", "probability": 0.7998046875}, {"start": 2895.38, "end": 2895.7, "word": " Micro", "probability": 0.4345703125}, {"start": 2895.7, "end": 2896.64, "word": " Aerophilic", "probability": 0.7799072265625}, {"start": 2896.64, "end": 2897.22, "word": " Bacteria", "probability": 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2911.1, "word": " محكم", "probability": 0.9021809895833334}, {"start": 2911.1, "end": 2911.78, "word": " الإغلاق", "probability": 0.9888916015625}], "temperature": 1.0}, {"id": 114, "seek": 293859, "start": 2912.27, "end": 2938.59, "text": "بحط فيه اللي هي ال medium البكتيريا اللي انا او الأطباق البكتيريا اللي انا زرعتها على ال media على الطبق اللي بتحتاج ظروف او بتحتاج جميعة قليلة من ال oxygen بحطها upside down بقوللي اخر شئ بتحطي ال candle بتشعلي ال candle اللي هي الشمعة ممكن احنا نجيب slide", "tokens": [3555, 5016, 9566, 8978, 3224, 13672, 1829, 39896, 2423, 6399, 29739, 4117, 2655, 13546, 25528, 13672, 1829, 1975, 8315, 1975, 2407, 16247, 9566, 3555, 995, 4587, 29739, 4117, 2655, 13546, 25528, 13672, 1829, 1975, 8315, 30767, 2288, 34268, 11296, 15844, 2423, 3021, 15844, 41950, 3555, 4587, 13672, 1829, 39894, 33753, 26108, 1357, 116, 32887, 5172, 1975, 2407, 39894, 33753, 26108, 10874, 2304, 40228, 3660, 12174, 20292, 37977, 9154, 2423, 9169, 4724, 5016, 9566, 11296, 14119, 760, 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"probability": 0.787841796875}, {"start": 2915.61, "end": 2915.93, "word": " انا", "probability": 0.86328125}, {"start": 2915.93, "end": 2917.41, "word": " او", "probability": 0.487548828125}, {"start": 2917.41, "end": 2918.11, "word": " الأطباق", "probability": 0.91181640625}, {"start": 2918.11, "end": 2919.07, "word": " البكتيريا", "probability": 0.923046875}, {"start": 2919.07, "end": 2919.21, "word": " اللي", "probability": 0.949951171875}, {"start": 2919.21, "end": 2919.35, "word": " انا", "probability": 0.80859375}, {"start": 2919.35, "end": 2920.83, "word": " زرعتها", "probability": 0.8751220703125}, {"start": 2920.83, "end": 2921.07, "word": " على", "probability": 0.74365234375}, {"start": 2921.07, "end": 2921.57, "word": " ال", "probability": 0.9541015625}, {"start": 2921.57, "end": 2922.13, "word": " media", "probability": 0.477783203125}, {"start": 2922.13, "end": 2922.39, "word": " على", "probability": 0.83935546875}, {"start": 2922.39, "end": 2923.23, "word": " الطبق", "probability": 0.9381510416666666}, {"start": 2923.23, "end": 2924.17, "word": " اللي", "probability": 0.932861328125}, {"start": 2924.17, "end": 2924.99, "word": " بتحتاج", "probability": 0.8899739583333334}, {"start": 2924.99, "end": 2925.67, "word": " ظروف", "probability": 0.912353515625}, {"start": 2925.67, "end": 2926.09, "word": " او", "probability": 0.82763671875}, {"start": 2926.09, "end": 2926.93, "word": " بتحتاج", "probability": 0.823486328125}, {"start": 2926.93, "end": 2927.31, "word": " جميعة", "probability": 0.65850830078125}, {"start": 2927.31, "end": 2927.65, "word": " قليلة", "probability": 0.93896484375}, {"start": 2927.65, "end": 2927.81, "word": " من", "probability": 0.9931640625}, {"start": 2927.81, "end": 2927.93, "word": " ال", "probability": 0.861328125}, {"start": 2927.93, "end": 2928.31, "word": " oxygen", "probability": 0.708984375}, {"start": 2928.31, "end": 2928.81, "word": " بحطها", "probability": 0.964111328125}, {"start": 2928.81, "end": 2929.15, 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{"start": 3099.56, "end": 3101.0, "word": " بدخل", "probability": 0.9093424479166666}, {"start": 3101.0, "end": 3101.4, "word": " فقط", "probability": 0.98876953125}, {"start": 3101.4, "end": 3101.58, "word": " او", "probability": 0.938232421875}, {"start": 3101.58, "end": 3101.82, "word": " اني", "probability": 0.4825439453125}, {"start": 3101.82, "end": 3101.98, "word": " انا", "probability": 0.824462890625}, {"start": 3101.98, "end": 3102.74, "word": " بشتغل", "probability": 0.9857421875}, {"start": 3102.74, "end": 3103.3, "word": " من", "probability": 0.998046875}, {"start": 3103.3, "end": 3103.74, "word": " خلال", "probability": 0.99560546875}, {"start": 3103.74, "end": 3104.36, "word": " قفازات", "probability": 0.94287109375}, {"start": 3104.36, "end": 3104.92, "word": " متطيئة", "probability": 0.8099609375}, {"start": 3104.92, "end": 3105.02, "word": " زي", "probability": 0.621826171875}, {"start": 3105.02, "end": 3105.1, "word": " ما", "probability": 0.673828125}, {"start": 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{"start": 3109.36, "end": 3109.64, "word": " انا", "probability": 0.92236328125}, {"start": 3109.64, "end": 3110.08, "word": " بقدر", "probability": 0.994140625}, {"start": 3110.08, "end": 3110.58, "word": " اشتغل", "probability": 0.95283203125}, {"start": 3110.58, "end": 3110.92, "word": " داخل", "probability": 0.98193359375}, {"start": 3110.92, "end": 3111.2, "word": " هذا", "probability": 0.97607421875}, {"start": 3111.2, "end": 3111.36, "word": " ال", "probability": 0.95556640625}, {"start": 3111.36, "end": 3111.68, "word": " chamber", "probability": 0.98388671875}], "temperature": 1.0}, {"id": 122, "seek": 313641, "start": 3112.29, "end": 3136.41, "text": "هك بيوفر طبعا او بمنع دخول ال oxygen لداخل ال chamber بالنسبة للظروف اللي لاهوائية كيف بتتكون طبعا في انديهيدروجين جاز بيصير تفاعل بين ال hydrogen gas و ال oxygen اللي موجود داخل ال chamber هك بتخلص من اللي هو ال oxygen بوفر ظروف CO2 بالتالي بتتوفر الظروف اللي لاهوائية", "tokens": [3224, 4117, 4724, 1829, 38688, 2288, 23032, 3555, 3615, 995, 1975, 2407, 4724, 27842, 3615, 11778, 9778, 12610, 2423, 9169, 5296, 3215, 47283, 1211, 2423, 13610, 20666, 1863, 35457, 3660, 24976, 19913, 32887, 5172, 13672, 1829, 20193, 3224, 2407, 16373, 10632, 9122, 33911, 39894, 2655, 30544, 23032, 3555, 3615, 995, 8978, 16472, 16254, 3224, 25708, 2288, 29245, 9957, 10874, 31377, 4724, 1829, 9381, 13546, 6055, 5172, 995, 30241, 49374, 2423, 12697, 4211, 4032, 2423, 9169, 13672, 1829, 3714, 29245, 23328, 11778, 47283, 1211, 2423, 13610, 8032, 4117, 39894, 9778, 1211, 9381, 9154, 13672, 1829, 31439, 2423, 9169, 4724, 38688, 2288, 1357, 116, 32887, 5172, 3002, 17, 20666, 2655, 6027, 1829, 39894, 2655, 38688, 2288, 6024, 116, 32887, 5172, 13672, 1829, 20193, 3224, 2407, 16373, 10632], "avg_logprob": -0.14967758149381669, "compression_ratio": 1.9429824561403508, "no_speech_prob": 6.67572021484375e-06, "words": [{"start": 3112.29, "end": 3112.69, "word": "هك", "probability": 0.654052734375}, {"start": 3112.69, "end": 3113.19, "word": " بيوفر", "probability": 0.748046875}, {"start": 3113.19, "end": 3113.63, "word": " طبعا", "probability": 0.9263916015625}, {"start": 3113.63, "end": 3113.97, "word": " او", "probability": 0.8408203125}, {"start": 3113.97, "end": 3114.79, "word": " بمنع", "probability": 0.951171875}, {"start": 3114.79, "end": 3115.23, "word": " دخول", "probability": 0.9923502604166666}, {"start": 3115.23, "end": 3115.35, "word": " ال", "probability": 0.75830078125}, {"start": 3115.35, "end": 3115.71, "word": " oxygen", "probability": 0.44921875}, {"start": 3115.71, "end": 3116.43, "word": " لداخل", "probability": 0.9862060546875}, {"start": 3116.43, "end": 3116.57, "word": " ال", "probability": 0.87890625}, {"start": 3116.57, "end": 3116.89, "word": " chamber", "probability": 0.9775390625}, {"start": 3116.89, "end": 3118.37, "word": " بالنسبة", "probability": 0.83709716796875}, {"start": 3118.37, "end": 3119.57, "word": " للظروف", "probability": 0.870849609375}, {"start": 3119.57, "end": 3119.71, "word": " اللي", "probability": 0.758056640625}, {"start": 3119.71, "end": 3120.23, "word": " لاهوائية", "probability": 0.727294921875}, {"start": 3120.23, "end": 3120.51, "word": " كيف", "probability": 0.798828125}, {"start": 3120.51, "end": 3121.15, "word": " بتتكون", "probability": 0.8984375}, {"start": 3121.15, "end": 3121.95, "word": " طبعا", "probability": 0.9427490234375}, {"start": 3121.95, "end": 3122.09, "word": " في", "probability": 0.8798828125}, {"start": 3122.09, "end": 3123.01, "word": " انديهيدروجين", "probability": 0.7501395089285714}, {"start": 3123.01, "end": 3123.47, "word": " جاز", "probability": 0.798095703125}, {"start": 3123.47, "end": 3124.13, "word": " بيصير", "probability": 0.9481201171875}, {"start": 3124.13, "end": 3124.61, "word": " تفاعل", "probability": 0.9324951171875}, {"start": 3124.61, "end": 3124.83, "word": " بين", "probability": 0.96337890625}, {"start": 3124.83, "end": 3124.97, "word": " ال", "probability": 0.984375}, {"start": 3124.97, "end": 3125.37, "word": " hydrogen", "probability": 0.69287109375}, {"start": 3125.37, "end": 3125.85, "word": " gas", "probability": 0.7548828125}, {"start": 3125.85, "end": 3126.01, "word": " و", "probability": 0.85546875}, {"start": 3126.01, "end": 3126.27, "word": " ال", "probability": 0.7333984375}, {"start": 3126.27, "end": 3126.67, "word": " oxygen", "probability": 0.87451171875}, {"start": 3126.67, "end": 3126.87, "word": " اللي", "probability": 0.81884765625}, {"start": 3126.87, "end": 3127.19, "word": " موجود", "probability": 0.9695638020833334}, {"start": 3127.19, "end": 3127.57, "word": " داخل", "probability": 0.96875}, {"start": 3127.57, "end": 3127.71, "word": " ال", "probability": 0.96142578125}, {"start": 3127.71, "end": 3128.13, "word": " chamber", "probability": 0.984375}, {"start": 3128.13, "end": 3129.05, "word": " هك", "probability": 0.855712890625}, {"start": 3129.05, "end": 3129.73, "word": " بتخلص", "probability": 0.9659423828125}, {"start": 3129.73, "end": 3130.07, "word": " من", "probability": 0.98779296875}, {"start": 3130.07, "end": 3130.39, "word": " اللي", "probability": 0.97509765625}, {"start": 3130.39, "end": 3130.71, "word": " هو", "probability": 0.98828125}, {"start": 3130.71, "end": 3130.95, "word": " ال", "probability": 0.96875}, {"start": 3130.95, "end": 3131.41, "word": " oxygen", "probability": 0.9375}, {"start": 3131.41, "end": 3132.01, "word": " بوفر", "probability": 0.85986328125}, {"start": 3132.01, "end": 3132.43, "word": " ظروف", "probability": 0.9375}, {"start": 3132.43, "end": 3133.31, "word": " CO2", "probability": 0.79736328125}, {"start": 3133.31, "end": 3134.07, "word": " بالتالي", "probability": 0.82989501953125}, {"start": 3134.07, "end": 3134.87, "word": " بتتوفر", "probability": 0.944091796875}, {"start": 3134.87, "end": 3135.45, "word": " الظروف", "probability": 0.9744873046875}, {"start": 3135.45, "end": 3135.67, "word": " اللي", "probability": 0.95068359375}, {"start": 3135.67, "end": 3136.41, "word": " لاهوائية", "probability": 0.97919921875}], "temperature": 1.0}, {"id": 123, "seek": 315968, "start": 3137.72, "end": 3159.68, "text": "الباليديوم كان مهم كمحفز لإرتباط الـH2 مع الأكسجين عشان أتخلص من الأكسجين والذي يتكون عندى الـH2O هكذا نكون خلصنا الـchamber يعطيكم العافية والسلام عليكم ورحمة الله وبركاته", "tokens": [6027, 3555, 6027, 1829, 16254, 20498, 25961, 3714, 16095, 9122, 2304, 5016, 5172, 11622, 5296, 28814, 43500, 3555, 41193, 2423, 39184, 39, 17, 20449, 16247, 4117, 3794, 7435, 9957, 6225, 8592, 7649, 5551, 2655, 9778, 1211, 9381, 9154, 16247, 4117, 3794, 7435, 9957, 16070, 8848, 1829, 7251, 2655, 30544, 43242, 7578, 2423, 39184, 39, 17, 46, 8032, 4117, 15730, 8717, 30544, 16490, 1211, 9381, 8315, 2423, 39184, 339, 335, 607, 37495, 9566, 1829, 24793, 18863, 31845, 10632, 16070, 3794, 37440, 25894, 24793, 4032, 2288, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224], "avg_logprob": -0.3508064490492626, "compression_ratio": 1.627659574468085, "no_speech_prob": 1.7523765563964844e-05, "words": [{"start": 3137.72, "end": 3138.92, "word": "الباليديوم", "probability": 0.72900390625}, {"start": 3138.92, "end": 3139.1, "word": " كان", "probability": 0.271240234375}, {"start": 3139.1, "end": 3139.78, "word": " مهم", "probability": 0.6263427734375}, {"start": 3139.78, "end": 3142.58, "word": " كمحفز", "probability": 0.7439453125}, {"start": 3142.58, "end": 3144.58, "word": " لإرتباط", "probability": 0.7494140625}, {"start": 3144.58, "end": 3145.34, "word": " الـH2", "probability": 0.706298828125}, {"start": 3145.34, "end": 3146.06, "word": " مع", "probability": 0.369140625}, {"start": 3146.06, "end": 3146.88, "word": " الأكسجين", "probability": 0.8734375}, {"start": 3146.88, "end": 3147.2, "word": " عشان", "probability": 0.7039591471354166}, {"start": 3147.2, "end": 3147.86, "word": " أتخلص", "probability": 0.90126953125}, {"start": 3147.86, "end": 3148.1, "word": " من", "probability": 0.9833984375}, {"start": 3148.1, "end": 3148.78, "word": " الأكسجين", "probability": 0.8477783203125}, {"start": 3148.78, "end": 3148.88, "word": " والذي", "probability": 0.5523681640625}, {"start": 3148.88, "end": 3149.22, "word": " يتكون", "probability": 0.8232421875}, {"start": 3149.22, "end": 3149.84, "word": " عندى", "probability": 0.7236328125}, {"start": 3149.84, "end": 3151.74, "word": " الـH2O", "probability": 0.842236328125}, {"start": 3151.74, "end": 3152.66, "word": " هكذا", "probability": 0.5923055013020834}, {"start": 3152.66, "end": 3152.9, "word": " نكون", "probability": 0.324462890625}, {"start": 3152.9, "end": 3153.7, "word": " خلصنا", "probability": 0.9271240234375}, {"start": 3153.7, "end": 3155.3, "word": " الـchamber", "probability": 0.53193359375}, {"start": 3155.3, "end": 3156.6, "word": " يعطيكم", "probability": 0.67645263671875}, {"start": 3156.6, "end": 3157.18, "word": " العافية", "probability": 0.91845703125}, {"start": 3157.18, "end": 3157.74, "word": " والسلام", "probability": 0.91748046875}, {"start": 3157.74, "end": 3158.24, "word": " عليكم", "probability": 0.95458984375}, {"start": 3158.24, "end": 3158.7, "word": " ورحمة", "probability": 0.9727783203125}, {"start": 3158.7, "end": 3158.92, "word": " الله", "probability": 0.966796875}, {"start": 3158.92, "end": 3159.68, "word": " وبركاته", "probability": 0.9720703125}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 3161.4665, "duration_after_vad": 3093.5143749999866}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/UNVvGjbAdO4.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/UNVvGjbAdO4.srt
new file mode 100644
index 0000000000000000000000000000000000000000..418ac098c50c55ac133868ce89176632689f6288
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/UNVvGjbAdO4.srt
@@ -0,0 +1,862 @@
+1
+00:00:00,250 --> 00:00:04,010
+السلام عليكم ورحمة الله وبركاته. نستكمل معكم
+
+2
+00:00:04,010 --> 00:00:07,410
+محاضرات مساق Microbiology العملية في هذا
+
+3
+00:00:07,410 --> 00:00:10,470
+اللقاء. هتكون المحاضرة بعنوان Simple Stain.
+
+4
+00:00:10,470 --> 00:00:14,310
+هنتعرف على الصبغات وأنواع الصبغات التي من ضمنها
+
+5
+00:00:14,310 --> 00:00:17,990
+الـ Simple Stain. في المعمل السابق، تعرّفنا أن
+
+6
+00:00:17,990 --> 00:00:22,490
+البكتيريا حجمها صغير، لا تُرى بالعين المجردة، ويتراوح
+
+7
+00:00:22,490 --> 00:00:27,800
+حجمها ما بين 0.5 إلى 2 ميكرومتر. عشان أتغلب على هذه
+
+8
+00:00:27,800 --> 00:00:31,020
+المشكلة، استخدمت الميكروسكوب عشان يعملي
+
+9
+00:00:31,020 --> 00:00:34,940
+magnification، تكبير. أنا أقدر أشوفها. من الصفات
+
+10
+00:00:34,940 --> 00:00:38,300
+الأخرى، رؤية البكتيريا أنها transparent، شفافة،
+
+11
+00:00:38,300 --> 00:00:42,440
+colorless، ما إلها لون، featureless، ما إلها أي معالم.
+
+12
+00:00:42,440 --> 00:00:46,920
+عشان أتغلب على هذه المشاكل، استخدمت الـ stain، استخدمت
+
+13
+00:00:46,920 --> 00:00:51,320
+الصبغات حتى أقدر أميزها بشكل واضح وأتعرف على
+
+14
+00:00:51,320 --> 00:00:56,600
+شكلها. طيب، لو بدنا نعرف: What is a stain?
+
+15
+00:00:56,600 --> 00:01:00,260
+stain? A stain is a substance that adheres to a
+
+16
+00:01:00,260 --> 00:01:04,480
+cell, giving the cell color. هي مادة بتلتصق بالخلايا
+
+17
+00:01:04,480 --> 00:01:08,880
+وبتعطي الخلايا لونًا. اتفقنا أن البكتيريا colorless.
+
+18
+00:01:08,880 --> 00:01:13,300
+من خلال الـ stain، هتصير colored، تعطيها لونًا. وجود هذا
+
+19
+00:01:13,300 --> 00:01:16,560
+اللون، the presence of color، gives the cell
+
+20
+00:01:16,560 --> 00:01:20,650
+significant contrast so are much more visible. وجود
+
+21
+00:01:20,650 --> 00:01:25,290
+هذا اللون، سيجعل الخلايا أكثر تباينًا ووضوحًا وسهل
+
+22
+00:01:25,290 --> 00:01:30,650
+رؤيتها والتعرف على شكلها. طيب، لو احنا سألنا: كيف
+
+23
+00:01:30,650 --> 00:01:35,010
+الـ stain، كيف الصبغة بتلتصق بالخلايا، بالـ cell، أو ليش
+
+24
+00:01:35,010 --> 00:01:39,870
+الصبغة بتصبغ البكتيريا وما بتصبغ أشياء أخرى في الوسط.
+
+25
+00:01:39,870 --> 00:01:43,610
+بقولنا أن في عندي قوة ارتباط، affinity، ما بين
+
+26
+00:01:43,610 --> 00:01:48,050
+الـ stain وما بين الـ cell. طيب، شو سبب قوة الارتباط هذه؟
+
+27
+00:01:48,960 --> 00:01:54,260
+احنا بنعرف أن البكتيريا فيها مكونات. هذه المكونات
+
+28
+00:01:54,260 --> 00:01:58,940
+في بعضها يحمل شحنة موجبة، والبعض الآخر يحمل شحنة
+
+29
+00:01:58,940 --> 00:02:03,360
+سالبة. لكن الشحنة الكلية للبكتيريا، الـ overall
+
+30
+00:02:03,360 --> 00:02:08,320
+charge، تعتبر negative charge، شحنة سالبة. واحنا
+
+31
+00:02:08,320 --> 00:02:13,690
+عارفين أن الشحنات المختلفة تتجاذب. عشان يصير تجاذب ما
+
+32
+00:02:13,690 --> 00:02:17,670
+بين الـ السل، ما بين الخلايا، ما بين البكتيريا، وما بين
+
+33
+00:02:17,670 --> 00:02:23,650
+الصبغة، الـ stain، لازم تكون شحنة الصبغة شحنة مخالفة
+
+34
+00:02:23,650 --> 00:02:28,590
+لشحنة البكتيريا. يبقى لازم تكون شحنة الصبغة شحنة
+
+35
+00:02:28,590 --> 00:02:33,590
+موجبة، يعني basic stain. بالتالي بيصير في عندي تجاذب
+
+36
+00:02:33,590 --> 00:02:37,900
+وارتباط، وبتلتصق الصبغة على الخلايا. يبقى شحنة
+
+37
+00:02:37,900 --> 00:02:42,240
+البكتيريا سالبة، عشان هيك أنا بستخدم صبغات موجبة، basic stain.
+
+38
+00:02:42,240 --> 00:02:48,060
+طيب، الشحنة، صبغات basic stain. طب لو عندي أنا organism
+
+39
+00:02:48,060 --> 00:02:52,060
+غير البكتيريا، شحنته الكلية موجبة، بقى شو هستخدم؟
+
+40
+00:02:52,060 --> 00:02:57,660
+هستخدم صبغات سالبة الشحنة، هستخدم acidic stain. يبقى
+
+41
+00:02:57,660 --> 00:03:00,940
+في عندي أنا different stains. اتكلمنا، قولنا في عندي
+
+42
+00:03:00,940 --> 00:03:04,990
+acidic stain، في عندي basic stain.  They have different
+
+43
+00:03:04,990 --> 00:03:08,330
+affinities for different organisms، اللي هي قوة
+
+44
+00:03:08,330 --> 00:03:13,230
+ارتباط مختلفة بالكائنات المختلفة. عندي أنا الـ basic
+
+45
+00:03:13,230 --> 00:03:17,410
+stain بتصبغ كائنات بتحمل شحنة سالبة. الـ acidic
+
+46
+00:03:17,410 --> 00:03:21,790
+stain بتصبغ كائنات بتحمل شحنة موجبة. يعني يبقى قوة
+
+47
+00:03:21,790 --> 00:03:26,950
+ارتباط مختلفة لكائنات مختلفة، or different parts of
+
+48
+00:03:26,950 --> 00:03:31,430
+an organism. طيب، لو أنا بدي أصبغ مكون داخل الخلية،
+
+49
+00:03:31,430 --> 00:03:35,820
+لسبيل المثال، الـ protein، وقولنا حسب هذا المكون، والله
+
+50
+00:03:35,820 --> 00:03:40,600
+إذا شحنة هذا المكون سالبة، بصبغه بشحنات موجبة،
+
+51
+00:03:40,600 --> 00:03:46,420
+بصبغه بأصباغ موجبة، basic stain. لو شحنة هذا
+
+52
+00:03:46,420 --> 00:03:51,700
+المكون موجبة، بصبغه بصبغات سالبة، acidic stain.
+
+53
+00:03:51,700 --> 00:03:57,160
+They are used to differentiate different types of
+
+54
+00:03:57,160 --> 00:04:02,090
+organisms or to view specific parts of an organism. قلنا
+
+55
+00:04:02,090 --> 00:04:06,610
+أن الـ stain بتعطي الخلايا لونًا، وهذا يجعل رؤيتها
+
+56
+00:04:06,610 --> 00:04:10,810
+وتمييزها والتعرف على شكلها أسهل. لكن بقول في عند
+
+57
+00:04:10,810 --> 00:04:15,630
+استخدامات أخرى للـ stain. استخدام آخر، to differentiate
+
+58
+00:04:15,630 --> 00:04:20,010
+different types of organisms، عشان يفرق بين أنواع
+
+59
+00:04:20,010 --> 00:04:24,710
+مختلفة من الكائنات. بسمي هذا النوع من الصبغات التي
+
+60
+00:04:24,710 --> 00:04:29,290
+تميز بين أنواع مختلفة من الكائنات، differential
+
+61
+00:04:30,540 --> 00:04:35,620
+stain. تمام، هنتعرف عليها بالتفصيل في معامل لاحقة. هاي الـ
+
+62
+00:04:35,620 --> 00:04:41,760
+differential stain بتقسم البكتيريا لمجموعات، or
+
+63
+00:04:41,760 --> 00:04:45,320
+to view specific parts of an organism، أو أني أنا أشوف
+
+64
+00:04:45,320 --> 00:04:49,480
+مكونًا من مكونات الـ organism. وهي الصبغات التي أسميها
+
+65
+00:04:49,480 --> 00:04:54,460
+special stain، بتصبغ مكونًا خاصًا في الخلية البكتيرية.
+
+66
+00:04:54,460 --> 00:04:57,260
+على سبيل المثال، بدي أصبغ الـ spore، الـ endospore
+
+67
+00:04:57,260 --> 00:05:02,520
+stain، تعتبر special stain. بدي أشوف مكونًا خاصًا داخل
+
+68
+00:05:02,520 --> 00:05:07,960
+الخلية البكتيرية. هنا قلنا أن الـ stain بستخدمها أنا
+
+69
+00:05:07,960 --> 00:05:12,520
+عشان أتعرف على شكل البكتيريا، bacterial morphology.
+
+70
+00:05:12,520 --> 00:05:16,860
+يبقى الـ stain بشكل عام تُستخدم حتى أني أنا أتعرف على الـ
+
+71
+00:05:16,860 --> 00:05:20,800
+bacterial morphology. بقولي الـ bacterial morphology
+
+72
+00:05:20,800 --> 00:05:24,680
+ممكن أتعرف عليه مش بس من خلال الصبغات، في طرق ثانية.
+
+73
+00:05:24,680 --> 00:05:29,340
+الطريقة الثانية اسمها الـ wet mount. هذه الطريقة،
+
+74
+00:05:29,340 --> 00:05:34,380
+البكتيريا اللي أنا بشوفها بتكون living، بتكون حية،
+
+75
+00:05:34,380 --> 00:05:40,380
+organisms، غير مصبوغة. كيف أنا بعمل هذه الطريقة؟ بجيب
+
+76
+00:05:40,380 --> 00:05:44,380
+slide وبحط drop of normal saline. normal saline هو
+
+77
+00:05:44,380 --> 00:05:51,360
+محلول ملحي بتركيز 0.85% من 100%. هذا المحلول يتم
+
+78
+00:05:51,360 --> 00:05:53,080
+تحضيره بطريقة معينة.
+
+79
+00:05:55,470 --> 00:05:58,850
+وبيحافظ على الـ osmotic pressure، على الضغط
+
+80
+00:05:58,850 --> 00:06:02,310
+الاسموذي. بعد ما أنا أحط drop من الـ normal saline،
+
+81
+00:06:02,310 --> 00:06:05,690
+بجيب touch من البكتيريا. من وين بجيب الـ touch من
+
+82
+00:06:05,690 --> 00:06:09,010
+البكتيريا؟ هذا بيكون في طبعًا عندي طبق Petri dish،
+
+83
+00:06:09,010 --> 00:06:13,230
+بطلعه من الـ incubator، من الحاضنة، وبأخذ touch من
+
+84
+00:06:13,230 --> 00:06:17,190
+البكتيريا باستخدام الـ loop، سواء كان بلاستيك أو معدني،
+
+85
+00:06:17,190 --> 00:06:22,080
+nichrome loop، ولازم يكون طبعًا معقم، معقّم. باستخدام
+
+86
+00:06:22,080 --> 00:06:26,520
+بأخذ البكتيريا من طبق، هذا الطبق Petri dish، و
+
+87
+00:06:26,520 --> 00:06:31,980
+باستخدام الـ loop. بعدها بعمل mix خفيف بين الـ normal
+
+88
+00:06:31,980 --> 00:06:36,740
+saline فيه. وبعد ما أخلص، بحط الـ cover slip، وتحت الـ
+
+89
+00:06:36,740 --> 00:06:41,000
+microscope بشوف بكتيريا living، حية، لأن أنا طلعت
+
+90
+00:06:41,000 --> 00:06:45,200
+البكتيريا من الـ incubator. تمام، understand؟ لأنه أنا
+
+91
+00:06:45,200 --> 00:06:49,340
+ما استخدمت أي صبغة خلال خطوات شغلي، فبالتالي مش
+
+92
+00:06:49,340 --> 00:06:53,880
+هتكون مسبوقة. نفس الفكرة لما كنتوا تعملوها في الـ
+
+93
+00:06:53,880 --> 00:06:57,620
+urine analysis، كنتوا تعملوا centrifugation للعينة،
+
+94
+00:06:57,620 --> 00:07:00,780
+تتخلصوا من الـ supernatant وتاخدوا الـ sediment،
+
+95
+00:07:00,780 --> 00:07:04,060
+وتحطوه على الـ slide، و cover slip، وتحت الميكروسكوب،
+
+96
+00:07:04,060 --> 00:07:08,600
+بتشوفوا البكتيريا living، حية، لأنك أنت ما عملت أي
+
+97
+00:07:08,600 --> 00:07:12,260
+خطوة قتلت البكتيريا، كانت في العينة unstained،
+
+98
+00:07:12,260 --> 00:07:15,440
+ما صبغتيش، ما استخدمتيش أي صباغة. يبقى هاي الطريقة
+
+99
+00:07:15,440 --> 00:07:19,920
+بسميها wet mount. الطريقة الثانية حتى أني أنا أتعرف
+
+100
+00:07:19,920 --> 00:07:22,980
+على الـ bacterial morphology، اللي هي طريقة الصباغة،
+
+101
+00:07:22,980 --> 00:07:26,600
+اللي احنا في هذا المعمل هنتعرف عليها خلال هذه
+
+102
+00:07:26,600 --> 00:07:30,080
+الطريقة. الـ organism بيكون killed، stained organism،
+
+103
+00:07:30,080 --> 00:07:35,420
+بيكون ميت. ليش؟ لأنه من ضمن خطوات الصباغة، يتم
+
+104
+00:07:35,420 --> 00:07:40,420
+تمرير الـ slide ثلاث مرات على الـ flame. هذه الخطوة بتعمل
+
+105
+00:07:40,420 --> 00:07:45,730
+قتل البكتيريا. الـ stain، لأن أنا هستخدم الصبغات، هستخدم
+
+106
+00:07:45,730 --> 00:07:49,550
+الـ basic stain اللي بتحمل شحنة موجبة، اللي بتصبغ
+
+107
+00:07:49,550 --> 00:07:55,750
+البكتيريا سالبة الشحنة. In our laboratory, bacterial
+
+108
+00:07:55,750 --> 00:07:59,250
+morphology, form and structure may be examined in
+
+109
+00:07:59,250 --> 00:08:02,290
+two ways. زي ما اتفقنا يعني، دي طريقتين عشان أتعرف
+
+110
+00:08:02,290 --> 00:08:06,430
+على الـ bacterial morphology، by observing living
+
+111
+00:08:06,430 --> 00:08:11,250
+organisms. هي الطريقة اللي بسميها الـ wet mount. باستخدام
+
+112
+00:08:11,250 --> 00:08:15,830
+killed stain organisms، اللي هنتعرف عليها في هذا المعمل.
+
+113
+00:08:15,830 --> 00:08:20,630
+بما أنه صغير جدًا، إلى جانب أن البكتيريا حجمها صغير
+
+114
+00:08:20,630 --> 00:08:23,450
+جدا، وتغلبنا على هذه المشكلة باستخدام الميكروسكوب،
+
+115
+00:08:23,450 --> 00:08:27,970
+بقول لي أن البكتيريا almost are also almost
+
+116
+00:08:27,970 --> 00:08:32,210
+completely transparent، شفافة، colorless، ما إلها لون،
+
+117
+00:08:32,210 --> 00:08:35,450
+and featureless، ما إلها معالم، in their natural
+
+118
+00:08:35,450 --> 00:08:40,780
+status. عشان أحل هذه المشاكل، باستخدام الـ stain،
+
+119
+00:08:40,780 --> 00:08:44,200
+however, staining can make the structure of
+
+120
+00:08:44,200 --> 00:08:48,200
+bacteria more pronounced. بقولنا أن الصبغة بتجعل
+
+121
+00:08:48,200 --> 00:08:51,820
+البكتيريا أكثر وضوحًا، وبقدر أني أنا أميزها.
+
+122
+00:08:51,820 --> 00:08:55,780
+Different types of staining methods are used to make
+
+123
+00:08:55,780 --> 00:08:59,980
+the cells and their internal structures more
+
+124
+00:08:59,980 --> 00:09:04,820
+visible under the light microscope. في عندي أنواع
+
+125
+00:09:04,820 --> 00:09:09,500
+كثيرة من الصبغات تُستخدم لصباغة الخلايا والتراكيب
+
+126
+00:09:09,500 --> 00:09:14,700
+الداخلية، زي الأسواط، زي الـ spores، زي الـ capsules، وبقدر
+
+127
+00:09:14,700 --> 00:09:17,440
+أني أنا أشوفها تحت الـ light microscope.
+
+128
+00:09:25,870 --> 00:09:29,790
+Components، أو مكونات الصبغة. الصبغة من إيش بتتكون؟
+
+129
+00:09:29,790 --> 00:09:33,310
+بقولنا الـ stains are mixtures of chromogen and
+
+130
+00:09:33,310 --> 00:09:37,890
+auxochrome. يبقى مكونات الصبغة، الصبغة بتتكون من
+
+131
+00:09:37,890 --> 00:09:42,110
+مكونين. أول مكون اسمه الـ chromogen، والمكون الثاني
+
+132
+00:09:42,110 --> 00:09:47,150
+اسمه الـ auxochrome. الـ chromogen هو المادة الملونة،
+
+133
+00:09:47,150 --> 00:09:51,230
+أما الـ auxochrome هو اللي بيعطي الشحنة. احنا لما
+
+134
+00:09:51,230 --> 00:09:55,310
+عرفنا الـ stain، قلنا it is a substance that adheres
+
+135
+00:09:55,310 --> 00:10:00,150
+to a cell، بتلتصق بالخلايا. كيف بتلتصق؟ من خلال وجود
+
+136
+00:10:00,150 --> 00:10:06,270
+تجاذب ما بين الشحنات. يبقى الصبغة لازم تحمل شحنة،
+
+137
+00:10:06,270 --> 00:10:09,410
+عشان يصير هذا التجاذب بينها وبين البكتيريا. مين
+
+138
+00:10:09,410 --> 00:10:13,430
+اللي بيعطي الـ stain الشحنة؟ الـ auxochrome. الـ auxochrome
+
+139
+00:10:13,430 --> 00:10:17,870
+هو اللي بيحمل الشحنة. نكمل التعريف: giving the cell
+
+140
+00:10:17,870 --> 00:10:21,970
+color، بتعطي الخلايا اللون. مين بيعطي اللون، أو مين
+
+141
+00:10:21,970 --> 00:10:25,550
+المسؤول، أو مين المادة الملونة فيه الصبغة؟ الـ
+
+142
+00:10:25,550 --> 00:10:28,410
+chromogen. الـ chromogen هو المادة الملونة، والـ
+
+143
+00:10:28,410 --> 00:10:32,550
+auxochrome هو اللي بيعطي الشحنة. بقولنا الـ chromogen
+
+144
+00:10:32,550 --> 00:10:36,590
+بتكون من مكونات فرعية أيضًا، اللي هي الـ benzene
+
+145
+00:10:36,590 --> 00:10:40,900
+derivatives، زائد الـ chromophore. بالضبط، مين من الـ
+
+146
+00:10:40,900 --> 00:10:45,660
+chromogen اللي هو اللي بيعطي اللون؟ الـ chromophore.
+
+147
+00:10:45,660 --> 00:10:52,180
+الـ chromophore هو بالتحديد اللي بيعطي اللون للصبغة،
+
+148
+00:10:52,180 --> 00:10:55,240
+هو الـ coloring agent، أو إذا حكينا الـ chromogen
+
+149
+00:10:55,240 --> 00:10:58,660
+برضه مش غلط. مين بالتحديد في الـ chromogen؟ الـ
+
+150
+00:10:58,660 --> 00:11:03,940
+chromophore. بالنسبة للـ auxochrome، الـ auxochrome قلنا
+
+151
+00:11:03,940 --> 00:11:07,080
+اللي بيعطي الشحنة، gives positive or negative charge
+
+152
+00:11:07,080 --> 00:11:11,870
+to the chromogen. بيعطي شحنة موجبة طبعًا، أو بيحمل
+
+153
+00:11:11,870 --> 00:11:16,010
+الشحنة الموجبة إذا كانت الـ stain هي basic stain،
+
+154
+00:11:16,010 --> 00:11:20,930
+وبيعطي الشحنة السالبة إذا كانت الصبغة هي
+
+155
+00:11:20,930 --> 00:11:25,910
+acidic stain. The ionized stain is capable of
+
+156
+00:11:25,910 --> 00:11:29,550
+binding to cell structures with opposite charge.
+
+157
+00:11:29,550 --> 00:11:33,970
+وقلنا الـ ionized stain قادر على الارتباط بتراكيب
+
+158
+00:11:33,970 --> 00:11:37,750
+الخلايا عكس شحنتها، عشان يصير تجاذب. يبقى العين
+
+159
+00:11:38,130 --> 00:11:43,090
+البكتيريا شحنتها سالبة، المفترض تكون الصبغة بتحمل
+
+160
+00:11:43,090 --> 00:12:01,650
+شحنة عكسها، يعني موجبة، بتحمل شحنة موجبة، يعني basic stain. في
+
+161
+00:12:01,650 --> 00:12:05,350
+هذا الـ slide، هنتعرف على أنواع الصبغات على حسب الـ
+
+162
+00:12:05,350 --> 00:12:10,880
+charge. وقولنا أنه بتنقسم لـ Basic Stain ولـ Acidic
+
+163
+00:12:10,880 --> 00:12:14,840
+Stain. نيجي نتعرف على الـ Basic Stain والـ Acidic
+
+164
+00:12:14,840 --> 00:12:19,280
+Stain. Basic Stain are cationic، يعني بيحمل شحنة
+
+165
+00:12:19,280 --> 00:12:22,580
+موجبة. When ionized, the chromogen exhibits a
+
+166
+00:12:22,580 --> 00:12:26,580
+positive charge. بيحمل شحنة موجبة. بما إنه بيحمل
+
+167
+00:12:26,580 --> 00:12:31,560
+شحنة موجبة، يبقى لازم يرتبط بتراكيب شحنتها سالبة.
+
+168
+00:12:31,850 --> 00:12:35,250
+Basic Stain binds to negatively charged cell
+
+169
+00:12:35,250 --> 00:12:39,810
+structures. زي مين هذه المكونات التي شحنتها سالبة؟
+
+170
+00:12:39,810 --> 00:12:44,590
+Like nucleic acids. هذه كلها أمثلة على أنواع من الـ
+
+171
+00:12:44,590 --> 00:12:49,050
+Basic Stain. أمثلة: methylene blue، crystal violet، safranin،
+
+172
+00:12:49,
+
+223
+00:16:27,350 --> 00:16:32,990
+Stain بتصبغ مكونات خاصة داخل الخلية البكتيرية من
+
+224
+00:16:32,990 --> 00:16:36,410
+الأمثلة على الـ special stain: كبسولر stain اللي هو
+
+225
+00:16:36,410 --> 00:16:41,110
+الحافظة، endospore stain الأبوغ، و flagella stain الفلاجلة
+
+226
+00:16:41,110 --> 00:16:45,120
+هنتعرف عليهم بالتفصيل، كلّ واحد و طريقة صباغته. أحنا قلنا
+
+227
+00:16:45,120 --> 00:16:49,260
+في أول slide إن أنا بستخدم الصبغات و differentiate
+
+228
+00:16:49,260 --> 00:16:53,400
+between different organisms، و أحنا قلنا إن هاي
+
+229
+00:16:53,400 --> 00:16:57,360
+معناها أو بنقصد فيها differential stain: a way to
+
+230
+00:16:57,360 --> 00:17:00,540
+view specific parts of the organism، و بنقصد فيها الـ
+
+231
+00:17:00,540 --> 00:17:04,380
+special stain. يبقى هاي استخدامات الـ stain بالإضافة
+
+232
+00:17:04,380 --> 00:17:09,350
+إني من خلالها بقدر أتعرف على الـ morphology، زي الـ
+
+233
+00:17:09,350 --> 00:17:11,750
+shape. بقدر أتعرف على الـ morphology لـ
+
+234
+00:17:11,750 --> 00:17:16,030
+البكتيريا، شكل البكتيريا، و الـ arrangement لخلايا
+
+235
+00:17:16,030 --> 00:17:20,650
+البكتيريا. هذا بالنسبة لأنواع الصبغات اللي
+
+236
+00:17:20,650 --> 00:17:28,090
+بنستخدمها في معمل الـ microbiology. حتى
+
+237
+00:17:28,090 --> 00:17:32,390
+الجدول ذاكر اللي هم كل الصبغات: الـ simple stain، الـ
+
+238
+00:17:32,390 --> 00:17:34,750
+الـ differential stain و أنواعها، و الـ
+
+239
+00:17:34,750 --> 00:17:38,110
+special stain و أنواعها. لكن القدر مش مطلوب، معناه
+
+240
+00:17:38,350 --> 00:17:42,350
+فقط هنتكلم على الـ simple stain بعد ما نخلص كل
+
+241
+00:17:42,350 --> 00:17:45,530
+الـ differential و الـ special stain. هذا
+
+242
+00:17:45,530 --> 00:17:50,450
+بيكون الجدول ملخص لإيه اللي هم كلهم، بس هنتكلم في
+
+243
+00:17:50,450 --> 00:17:55,010
+هذا اللقاء على أول صبغة اللي هي الـ simple stain.
+
+244
+00:17:55,010 --> 00:17:57,630
+ذاكر جون بالـ simple stain، يعني أنا بستخدم
+
+245
+00:17:57,630 --> 00:18:01,130
+methylene blue, carbol fuchsin, crystal violet،
+
+246
+00:18:01,130 --> 00:18:04,770
+و safranin. لو متذكرين إن هدول الصبغات هم كلهم
+
+247
+00:18:04,770 --> 00:18:08,530
+يعتبروا basic stain. والـ principle بتاع الـ
+
+248
+00:18:08,530 --> 00:18:11,630
+simple stain، يعني أنا بصبغ بـ basic stain بتحمل شحنة
+
+249
+00:18:11,630 --> 00:18:15,110
+موجبة، لإن البكتيريا الشحنة الكلية ليها شحنة سالبة.
+
+250
+00:18:15,110 --> 00:18:18,770
+ليه أنا بستخدم الـ simple stain؟ used to highlight
+
+251
+00:18:18,770 --> 00:18:22,310
+microorganisms to determine cellular shape and
+
+252
+00:18:22,310 --> 00:18:26,410
+arrangement. بقول لي الهدف من الـ simple stain يعني أنا
+
+253
+00:18:26,410 --> 00:18:29,410
+أتعرف على شكل و ترتيب الخلايا، الشكل و الـ
+
+254
+00:18:29,410 --> 00:18:35,130
+arrangement. بستخدم في الـ simple stain single basic
+
+255
+00:18:35,130 --> 00:18:40,590
+dye. بتصبغ الخلايا باستخدام فقط مكون واحد، أو
+
+256
+00:18:40,590 --> 00:18:44,070
+reaction واحد، اللي هو الـ basic stain. ممكن أستخدم
+
+257
+00:18:44,070 --> 00:18:47,530
+methylene blue، ممكن أستخدم carbol fuchsin، ممكن أستخدم
+
+258
+00:18:47,530 --> 00:18:51,610
+crystal violet، أو safranin، أو malachite green. المهم
+
+259
+00:18:51,610 --> 00:18:55,570
+يكون basic stain. ممنوع أنا أستخدم acidic stain.
+
+260
+00:18:55,570 --> 00:18:59,030
+بقول لك في بعض الأحيان ممكن نستخدم mordant. إيش يعني
+
+261
+00:18:59,030 --> 00:19:02,810
+mordant؟ mordant يعني مثبت، عشان يثبت لي اللون.
+
+262
+00:19:03,160 --> 00:19:06,480
+الـ mordant، مثال عليه الـ iodine. ممكن نستخدمه، ممكن
+
+263
+00:19:06,480 --> 00:19:11,020
+لأ، حسب إذا هو موجود، متوافر أو لأ. تمام، هذا بالنسبة
+
+264
+00:19:11,020 --> 00:19:14,900
+للـ simple stain. الـ differential stain، قلنا بتميّز
+
+265
+00:19:14,900 --> 00:19:18,400
+البكتيريا، أو بتقسمها لمجموعات. والـ special stain
+
+266
+00:19:18,400 --> 00:19:22,200
+بتصبغ أجزاء خاصة في الخلايا البكتيرية.
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/UNVvGjbAdO4_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/UNVvGjbAdO4_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..bb112e7edc1d79af1b1946b2f2799dbb6185fecc
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/UNVvGjbAdO4_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 2585, "start": 0.25, "end": 25.85, "text": "السلام عليكم ورحمة الله وبركاته نستكمل معاكم محاضرات مساق الـ Microbiology العملية في هذا اللقاء هتكون المحاضرة بعنوان الـ Simple Stain هنتعرف على الصبغات وأنواع الصبغات اللي من ضمنها الـ Simple Stain في المعمل السابق اتعرفنا أن البكتيريا حجمها صغير لاترى بالعين المجردة يتراوح حجمها ما بين مصر ل2 ميكرو متر", "tokens": [6027, 3794, 37440, 25894, 24793, 4032, 2288, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224, 8717, 14851, 24793, 1211, 20449, 995, 24793, 3714, 5016, 46958, 2288, 9307, 47524, 995, 4587, 2423, 39184, 5818, 20027, 1793, 18863, 42213, 10632, 8978, 23758, 13672, 4587, 16606, 8032, 2655, 30544, 9673, 5016, 46958, 25720, 45030, 1863, 2407, 7649, 2423, 39184, 21532, 745, 491, 8032, 29399, 3615, 28480, 15844, 31767, 3555, 17082, 9307, 4032, 33456, 14407, 3615, 31767, 3555, 17082, 9307, 13672, 1829, 9154, 48812, 27842, 11296, 2423, 39184, 21532, 745, 491, 8978, 9673, 25957, 1211, 21136, 16758, 4587, 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"probability": 0.93505859375}, {"start": 146.19, "end": 147.03, "word": " شحنة", "probability": 0.9915771484375}, {"start": 147.03, "end": 147.79, "word": " الصبغة", "probability": 0.9942626953125}, {"start": 147.79, "end": 148.59, "word": " شحنة", "probability": 0.937744140625}, {"start": 148.59, "end": 149.03, "word": " موجبة", "probability": 0.7906901041666666}, {"start": 149.03, "end": 149.69, "word": " يعني", "probability": 0.858642578125}, {"start": 149.69, "end": 150.69, "word": " basic", "probability": 0.72705078125}, {"start": 150.69, "end": 151.31, "word": " stain", "probability": 0.53173828125}, {"start": 151.31, "end": 152.21, "word": " بالتالي", "probability": 0.82977294921875}, {"start": 152.21, "end": 152.57, "word": " بيصير", "probability": 0.847900390625}, {"start": 152.57, "end": 152.71, "word": " في", "probability": 0.86181640625}, {"start": 152.71, "end": 152.97, "word": " عندي", "probability": 0.8876953125}, {"start": 152.97, "end": 153.59, "word": " تجاذب", "probability": 0.984619140625}, {"start": 153.59, "end": 154.27, "word": " وارتباط", "probability": 0.91630859375}, {"start": 154.27, "end": 155.03, "word": " وبتلتصق", "probability": 0.83740234375}, {"start": 155.03, "end": 155.71, "word": " الصبغة", "probability": 0.9945068359375}, {"start": 155.71, "end": 155.99, "word": " على", "probability": 0.8203125}, {"start": 155.99, "end": 156.73, "word": " الخلايا", "probability": 0.9685872395833334}], "temperature": 1.0}, {"id": 7, "seek": 18358, "start": 157.04, "end": 183.58, "text": "يبقى شحنة البكتيريا سالبة عشان هي أنا بستخدم صبغات موجة بت الشحنة صبغات basic stain طب لو عندي أنا organism غير البكتيريا شحنة الكلية موجة بقى شو هستخدم هستخدم صبغات سالبة الشحنة هستخدم acidic stain يبقى في عندي أنا different stain اتكلمنا قولنا في عندي acidic stain في عندي basic stain", "tokens": [1829, 3555, 4587, 7578, 13412, 5016, 1863, 3660, 29739, 4117, 2655, 13546, 25528, 8608, 6027, 49401, 6225, 8592, 7649, 39896, 41850, 4724, 14851, 9778, 40448, 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"probability": 0.9548828125}, {"start": 158.58, "end": 159.1, "word": " سالبة", "probability": 0.8235677083333334}, {"start": 159.1, "end": 159.82, "word": " عشان", "probability": 0.8893229166666666}, {"start": 159.82, "end": 160.02, "word": " هي", "probability": 0.68798828125}, {"start": 160.02, "end": 160.26, "word": " أنا", "probability": 0.357421875}, {"start": 160.26, "end": 160.98, "word": " بستخدم", "probability": 0.942138671875}, {"start": 160.98, "end": 161.66, "word": " صبغات", "probability": 0.9176025390625}, {"start": 161.66, "end": 162.08, "word": " موجة", "probability": 0.9474283854166666}, {"start": 162.08, "end": 162.24, "word": " بت", "probability": 0.8662109375}, {"start": 162.24, "end": 163.04, "word": " الشحنة", "probability": 0.79669189453125}, {"start": 163.04, "end": 163.96, "word": " صبغات", "probability": 0.9862060546875}, {"start": 163.96, "end": 164.56, "word": " basic", "probability": 0.7548828125}, {"start": 164.56, "end": 165.06, "word": " stain", "probability": 0.2305908203125}, {"start": 165.06, "end": 166.68, "word": " طب", "probability": 0.85986328125}, {"start": 166.68, "end": 166.82, "word": " لو", "probability": 0.97705078125}, {"start": 166.82, "end": 167.12, "word": " عندي", "probability": 0.78466796875}, {"start": 167.12, "end": 167.34, "word": " أنا", "probability": 0.73974609375}, {"start": 167.34, "end": 168.06, "word": " organism", "probability": 0.97412109375}, {"start": 168.06, "end": 168.44, "word": " غير", "probability": 0.9970703125}, {"start": 168.44, "end": 169.2, "word": " البكتيريا", "probability": 0.99130859375}, {"start": 169.2, "end": 170.08, "word": " شحنة", "probability": 0.97265625}, {"start": 170.08, "end": 170.66, "word": " الكلية", "probability": 0.5590006510416666}, {"start": 170.66, "end": 171.08, "word": " موجة", "probability": 0.990234375}, {"start": 171.08, "end": 171.24, "word": " بقى", "probability": 0.6411946614583334}, {"start": 171.24, "end": 171.4, "word": " شو", "probability": 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"start": 183.97, "end": 213.13, "text": "have different affinities for different organisms اللي هقوة ارتباط مختلفة بالكائنات المختلفة عندي انا ال basic stand بتصبخ كائنات بتحمل شحنة سالفة ال acidic stand بتصبخ كائنات بتحمل شحنة موجة يعني يبقى قوة ارتباط مختلفة لكائنات مختلفة or different part of organism طيب لو انا بدي أصبغ مكون داخل الخلية لسبيل مثل ال protein", "tokens": [24284, 819, 2096, 259, 1088, 337, 819, 22110, 13672, 1829, 8032, 4587, 2407, 3660, 1975, 43500, 3555, 41193, 3714, 46456, 46538, 3660, 20666, 4117, 16373, 8315, 2655, 9673, 46456, 46538, 3660, 18871, 16254, 1975, 8315, 2423, 3875, 1463, 39894, 9381, 3555, 9778, 9122, 16373, 8315, 2655, 39894, 35571, 1211, 13412, 5016, 1863, 3660, 8608, 6027, 5172, 3660, 2423, 39514, 1463, 39894, 9381, 3555, 9778, 9122, 16373, 8315, 2655, 39894, 35571, 1211, 13412, 5016, 1863, 3660, 3714, 29245, 3660, 37495, 22653, 7251, 3555, 4587, 7578, 12174, 2407, 3660, 1975, 43500, 3555, 41193, 3714, 46456, 46538, 3660, 5296, 4117, 16373, 8315, 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يبقى", "probability": 0.86041259765625}, {"start": 201.17, "end": 201.79, "word": " قوة", "probability": 0.9754231770833334}, {"start": 201.79, "end": 202.39, "word": " ارتباط", "probability": 0.9752197265625}, {"start": 202.39, "end": 203.21, "word": " مختلفة", "probability": 0.9906005859375}, {"start": 203.21, "end": 204.03, "word": " لكائنات", "probability": 0.9896484375}, {"start": 204.03, "end": 205.13, "word": " مختلفة", "probability": 0.99462890625}, {"start": 205.13, "end": 205.65, "word": " or", "probability": 0.84521484375}, {"start": 205.65, "end": 206.09, "word": " different", "probability": 0.92626953125}, {"start": 206.09, "end": 206.71, "word": " part", "probability": 0.6787109375}, {"start": 206.71, "end": 206.95, "word": " of", "probability": 0.98193359375}, {"start": 206.95, "end": 207.65, "word": " organism", "probability": 0.9111328125}, {"start": 207.65, "end": 208.43, "word": " طيب", "probability": 0.9576822916666666}, {"start": 208.43, "end": 208.59, "word": " لو", "probability": 0.9716796875}, {"start": 208.59, "end": 208.81, "word": " انا", "probability": 0.7900390625}, {"start": 208.81, "end": 209.07, "word": " بدي", "probability": 0.800537109375}, {"start": 209.07, "end": 209.59, "word": " أصبغ", "probability": 0.8203125}, {"start": 209.59, "end": 210.09, "word": " مكون", "probability": 0.826904296875}, {"start": 210.09, "end": 210.61, "word": " داخل", "probability": 0.9728190104166666}, {"start": 210.61, "end": 211.43, "word": " الخلية", "probability": 0.9635416666666666}, {"start": 211.43, "end": 212.09, "word": " لسبيل", "probability": 0.550537109375}, {"start": 212.09, "end": 212.59, "word": " مثل", "probability": 0.737548828125}, {"start": 212.59, "end": 212.71, "word": " ال", "probability": 0.78955078125}, {"start": 212.71, "end": 213.13, "word": " protein", "probability": 0.76220703125}], "temperature": 1.0}, {"id": 9, "seek": 24130, "start": 213.92, "end": 241.3, "text": "وقولنا حسب هذا المكون والله إذا شحنت هذا المكون صالبة بصبغ بيه شحنات موجبة بصبغ بيه أعفون صبغات موجبة basic stem لو شحنت هذا المكون موجبة بصبغ بيه صبغات صالبة بيه acidic stem there are used to differentiate different type of organism or to view specific part of organism", "tokens": [2407, 39648, 8315, 11331, 35457, 23758, 9673, 30544, 16070, 43761, 11933, 15730, 13412, 5016, 29399, 23758, 9673, 30544, 20328, 6027, 49401, 4724, 9381, 3555, 17082, 4724, 1829, 3224, 13412, 5016, 8315, 2655, 3714, 29245, 49401, 4724, 9381, 3555, 17082, 4724, 1829, 3224, 5551, 3615, 5172, 11536, 20328, 3555, 17082, 9307, 3714, 29245, 49401, 3875, 12312, 45164, 13412, 5016, 29399, 23758, 9673, 30544, 3714, 29245, 49401, 4724, 9381, 3555, 17082, 4724, 1829, 3224, 20328, 3555, 17082, 9307, 20328, 6027, 49401, 4724, 1829, 3224, 39514, 12312, 456, 366, 1143, 281, 23203, 819, 2010, 295, 24128, 420, 281, 1910, 2685, 644, 295, 24128], "avg_logprob": -0.13876856848745062, "compression_ratio": 2.0362694300518136, "no_speech_prob": 1.7881393432617188e-07, "words": [{"start": 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234.84, "end": 235.7, "word": " differentiate", "probability": 0.84716796875}, {"start": 235.7, "end": 236.32, "word": " different", "probability": 0.91845703125}, {"start": 236.32, "end": 236.84, "word": " type", "probability": 0.85595703125}, {"start": 236.84, "end": 237.16, "word": " of", "probability": 0.5556640625}, {"start": 237.16, "end": 237.78, "word": " organism", "probability": 0.919921875}, {"start": 237.78, "end": 238.52, "word": " or", "probability": 0.9345703125}, {"start": 238.52, "end": 238.84, "word": " to", "probability": 0.947265625}, {"start": 238.84, "end": 239.22, "word": " view", "probability": 0.583984375}, {"start": 239.22, "end": 239.84, "word": " specific", "probability": 0.8291015625}, {"start": 239.84, "end": 240.42, "word": " part", "probability": 0.86572265625}, {"start": 240.42, "end": 240.66, "word": " of", "probability": 0.97314453125}, {"start": 240.66, "end": 241.3, "word": " organism", "probability": 0.94482421875}], "temperature": 1.0}, {"id": 10, 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"probability": 0.59814453125}, {"start": 637.89, "end": 638.13, "word": " لـ", "probability": 0.805419921875}, {"start": 638.13, "end": 638.73, "word": " Chromophore", "probability": 0.77130126953125}], "temperature": 1.0}, {"id": 25, "seek": 66834, "start": 639.02, "end": 668.34, "text": "بالظبط مين من ال chromogen اللي هو اللي بيعطي اللون ال chromophore ال chromophore هو بالتحديد اللي بيعطي اللون للصبغة هو ال coloring agent أو إذا حكينا ال chromogen برضه مش غلط مين بالتحديد في ال chromogen ال chromophore بالنسبة لل exochrome ال exochrome قلنا اللي بيعطي الشحنة give positive or negative charge to the chromogen", "tokens": [3555, 6027, 19913, 3555, 9566, 3714, 9957, 9154, 2423, 16209, 8799, 13672, 1829, 31439, 13672, 1829, 4724, 40228, 9566, 1829, 13672, 11536, 2423, 16209, 5317, 418, 2423, 16209, 5317, 418, 31439, 20666, 2655, 5016, 16254, 3215, 13672, 1829, 4724, 40228, 9566, 1829, 13672, 11536, 24976, 9381, 3555, 17082, 3660, 31439, 2423, 23198, 9461, 34051, 11933, 15730, 11331, 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{"start": 642.26, "end": 642.68, "word": " هو", "probability": 0.5810546875}, {"start": 642.68, "end": 642.88, "word": " اللي", "probability": 0.93505859375}, {"start": 642.88, "end": 643.48, "word": " بيعطي", "probability": 0.8665771484375}, {"start": 643.48, "end": 644.1, "word": " اللون", "probability": 0.923095703125}, {"start": 644.1, "end": 644.62, "word": " ال", "probability": 0.50341796875}, {"start": 644.62, "end": 645.66, "word": " chromophore", "probability": 0.8050130208333334}, {"start": 645.66, "end": 645.8, "word": " ال", "probability": 0.2369384765625}, {"start": 645.8, "end": 647.26, "word": " chromophore", "probability": 0.8513997395833334}, {"start": 647.26, "end": 647.72, "word": " هو", "probability": 0.9677734375}, {"start": 647.72, "end": 648.66, "word": " بالتحديد", "probability": 0.9771484375}, {"start": 648.66, "end": 649.4, "word": " اللي", "probability": 0.9755859375}, {"start": 649.4, "end": 650.14, "word": " بيعطي", "probability": 0.9647216796875}, {"start": 650.14, "end": 650.72, "word": " اللون", "probability": 0.99365234375}, {"start": 650.72, "end": 652.18, "word": " للصبغة", "probability": 0.85400390625}, {"start": 652.18, "end": 652.56, "word": " هو", "probability": 0.89599609375}, {"start": 652.56, "end": 652.86, "word": " ال", "probability": 0.90283203125}, {"start": 652.86, "end": 653.22, "word": " coloring", "probability": 0.79638671875}, {"start": 653.22, "end": 653.9, "word": " agent", "probability": 0.92578125}, {"start": 653.9, "end": 654.12, "word": " أو", "probability": 0.59033203125}, {"start": 654.12, "end": 654.3, "word": " إذا", "probability": 0.849365234375}, {"start": 654.3, "end": 654.66, "word": " حكينا", "probability": 0.99072265625}, {"start": 654.66, "end": 654.76, "word": " ال", "probability": 0.490234375}, {"start": 654.76, "end": 655.24, "word": " chromogen", "probability": 0.8896484375}, {"start": 655.24, "end": 655.6, "word": " برضه", "probability": 0.9303385416666666}, {"start": 655.6, "end": 655.76, "word": " مش", "probability": 0.97802734375}, {"start": 655.76, "end": 656.06, "word": " غلط", "probability": 0.9864908854166666}, {"start": 656.06, "end": 656.24, "word": " مين", "probability": 0.808837890625}, {"start": 656.24, "end": 656.86, "word": " بالتحديد", "probability": 0.99013671875}, {"start": 656.86, "end": 657.52, "word": " في", "probability": 0.5234375}, {"start": 657.52, "end": 658.0, "word": " ال", "probability": 0.72216796875}, {"start": 658.0, "end": 658.48, "word": " chromogen", "probability": 0.927734375}, {"start": 658.48, "end": 658.66, "word": " ال", "probability": 0.390869140625}, {"start": 658.66, "end": 659.52, "word": " chromophore", "probability": 0.8914388020833334}, {"start": 659.52, "end": 661.4, "word": " بالنسبة", "probability": 0.976318359375}, {"start": 661.4, "end": 661.98, "word": " لل", "probability": 0.51123046875}, {"start": 661.98, "end": 662.88, "word": " exochrome", "probability": 0.65966796875}, {"start": 662.88, "end": 663.04, "word": " ال", "probability": 0.48486328125}, {"start": 663.04, "end": 663.62, "word": " exochrome", "probability": 0.8538411458333334}, {"start": 663.62, "end": 663.94, "word": " قلنا", "probability": 0.80126953125}, {"start": 663.94, "end": 664.08, "word": " اللي", "probability": 0.97900390625}, {"start": 664.08, "end": 664.46, "word": " بيعطي", "probability": 0.9556884765625}, {"start": 664.46, "end": 664.98, "word": " الشحنة", "probability": 0.983642578125}, {"start": 664.98, "end": 665.22, "word": " give", "probability": 0.8359375}, {"start": 665.22, "end": 665.72, "word": " positive", "probability": 0.8896484375}, {"start": 665.72, "end": 666.08, "word": " or", "probability": 0.83447265625}, {"start": 666.08, "end": 666.44, "word": " negative", "probability": 0.96923828125}, {"start": 666.44, "end": 667.08, "word": " charge", "probability": 0.978515625}, {"start": 667.08, "end": 667.36, "word": " to", "probability": 0.9765625}, {"start": 667.36, "end": 667.66, "word": " the", "probability": 0.87744140625}, {"start": 667.66, "end": 668.34, "word": " chromogen", "probability": 0.935302734375}], "temperature": 1.0}, {"id": 26, "seek": 69775, "start": 668.87, "end": 697.75, "text": "بيعطي شحنة موجبة طبعا أو بيحمل الشحنة الموجبة إذا كانت الستان هي basic stand وبيعطي الشحنة السالبة إذا كانت الصبغة اللي هي acidic stand the ionized stand is capable of binding to cell structure with opposite charge وقلنا ال ionized stand قادر على الإرتباط بتراكيب الخلايا عكس شحنتها عشان يصير تجارب يبقى عنين", "tokens": [21292, 3615, 9566, 1829, 13412, 5016, 1863, 3660, 3714, 29245, 49401, 23032, 3555, 3615, 995, 34051, 4724, 1829, 35571, 1211, 25124, 5016, 1863, 3660, 9673, 29245, 49401, 11933, 15730, 25961, 2655, 2423, 14851, 7649, 39896, 3875, 1463, 4032, 21292, 3615, 9566, 1829, 25124, 5016, 1863, 3660, 21136, 6027, 49401, 11933, 15730, 25961, 2655, 31767, 3555, 17082, 3660, 13672, 1829, 39896, 39514, 1463, 264, 17437, 1602, 1463, 307, 8189, 295, 17359, 281, 2815, 3877, 365, 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0.9569091796875}, {"start": 672.35, "end": 672.79, "word": " الموجبة", "probability": 0.9892578125}, {"start": 672.79, "end": 673.05, "word": " إذا", "probability": 0.938720703125}, {"start": 673.05, "end": 673.73, "word": " كانت", "probability": 0.983154296875}, {"start": 673.73, "end": 674.57, "word": " الستان", "probability": 0.7415364583333334}, {"start": 674.57, "end": 674.93, "word": " هي", "probability": 0.84375}, {"start": 674.93, "end": 675.53, "word": " basic", "probability": 0.5751953125}, {"start": 675.53, "end": 676.01, "word": " stand", "probability": 0.228271484375}, {"start": 676.01, "end": 677.15, "word": " وبيعطي", "probability": 0.9517578125}, {"start": 677.15, "end": 677.75, "word": " الشحنة", "probability": 0.9923095703125}, {"start": 677.75, "end": 678.37, "word": " السالبة", "probability": 0.8610026041666666}, {"start": 678.37, "end": 679.01, "word": " إذا", "probability": 0.9794921875}, {"start": 679.01, "end": 679.55, "word": " كانت", "probability": 0.994140625}, {"start": 679.55, "end": 680.27, "word": " الصبغة", "probability": 0.7779541015625}, {"start": 680.27, "end": 680.47, "word": " اللي", "probability": 0.85107421875}, {"start": 680.47, "end": 680.93, "word": " هي", "probability": 0.82275390625}, {"start": 680.93, "end": 681.77, "word": " acidic", "probability": 0.916015625}, {"start": 681.77, "end": 682.53, "word": " stand", "probability": 0.904296875}, {"start": 682.53, "end": 683.81, "word": " the", "probability": 0.2261962890625}, {"start": 683.81, "end": 684.57, "word": " ionized", "probability": 0.912109375}, {"start": 684.57, "end": 685.01, "word": " stand", "probability": 0.8125}, {"start": 685.01, "end": 685.25, "word": " is", "probability": 0.92529296875}, {"start": 685.25, "end": 685.61, "word": " capable", "probability": 0.97021484375}, {"start": 685.61, "end": 685.91, "word": " of", "probability": 0.9716796875}, {"start": 685.91, "end": 686.23, "word": " binding", "probability": 0.4638671875}, {"start": 686.23, "end": 686.53, "word": " to", "probability": 0.93603515625}, {"start": 686.53, "end": 686.75, "word": " cell", "probability": 0.814453125}, {"start": 686.75, "end": 687.29, "word": " structure", "probability": 0.728515625}, {"start": 687.29, "end": 687.65, "word": " with", "probability": 0.921875}, {"start": 687.65, "end": 688.35, "word": " opposite", "probability": 0.9580078125}, {"start": 688.35, "end": 689.55, "word": " charge", "probability": 0.79833984375}, {"start": 689.55, "end": 690.37, "word": " وقلنا", "probability": 0.7598876953125}, {"start": 690.37, "end": 690.57, "word": " ال", "probability": 0.56005859375}, {"start": 690.57, "end": 691.21, "word": " ionized", "probability": 0.854248046875}, {"start": 691.21, "end": 691.71, "word": " stand", "probability": 0.91357421875}, {"start": 691.71, "end": 692.23, "word": " قادر", "probability": 0.8951822916666666}, {"start": 692.23, "end": 692.45, "word": " على", "probability": 0.93994140625}, {"start": 692.45, "end": 693.17, "word": " الإرتباط", "probability": 0.9090576171875}, {"start": 693.17, "end": 693.97, "word": " بتراكيب", "probability": 0.93076171875}, {"start": 693.97, "end": 694.61, "word": " الخلايا", "probability": 0.96875}, {"start": 694.61, "end": 695.01, "word": " عكس", "probability": 0.9962565104166666}, {"start": 695.01, "end": 695.73, "word": " شحنتها", "probability": 0.933349609375}, {"start": 695.73, "end": 696.27, "word": " عشان", "probability": 0.9765625}, {"start": 696.27, "end": 696.61, "word": " يصير", "probability": 0.9152018229166666}, {"start": 696.61, "end": 697.13, "word": " تجارب", "probability": 0.90673828125}, {"start": 697.13, "end": 697.41, "word": " يبقى", "probability": 0.9163818359375}, {"start": 697.41, "end": 697.75, "word": " عنين", "probability": 0.820556640625}], "temperature": 1.0}, {"id": 27, "seek": 72581, "start": 698.13, "end": 725.81, "text": "البكتيريا شحنتها سالبة المفترض تكون الصبغة بتحمل شحنة عكسها يعني موجة بت الشحنة يعني basic step في هال slide هنتعرف على أنواع الصبغات على حسب ال charge", "tokens": [6027, 3555, 4117, 2655, 13546, 25528, 13412, 5016, 29399, 11296, 8608, 6027, 49401, 9673, 5172, 2655, 43042, 6055, 30544, 31767, 3555, 17082, 3660, 39894, 35571, 1211, 13412, 5016, 1863, 3660, 6225, 4117, 3794, 11296, 37495, 22653, 3714, 29245, 3660, 39894, 25124, 5016, 1863, 3660, 37495, 22653, 3875, 1823, 8978, 8032, 6027, 4137, 8032, 29399, 3615, 28480, 15844, 14739, 14407, 3615, 31767, 3555, 17082, 9307, 15844, 11331, 35457, 2423, 4602], "avg_logprob": -0.1535714213337217, "compression_ratio": 1.5421686746987953, "no_speech_prob": 5.364418029785156e-07, "words": [{"start": 698.13, "end": 698.91, "word": "البكتيريا", "probability": 0.9554850260416666}, {"start": 698.91, "end": 699.57, "word": " شحنتها", "probability": 0.9144287109375}, {"start": 699.57, "end": 700.11, "word": " سالبة", "probability": 0.8740234375}, {"start": 700.11, "end": 701.23, "word": " المفترض", "probability": 0.9468994140625}, {"start": 701.23, "end": 701.77, "word": " تكون", "probability": 0.85205078125}, {"start": 701.77, "end": 702.41, "word": " الصبغة", "probability": 0.95263671875}, {"start": 702.41, "end": 703.09, "word": " بتحمل", "probability": 0.8719075520833334}, {"start": 703.09, "end": 703.71, "word": " شحنة", "probability": 0.9541015625}, {"start": 703.71, "end": 704.17, "word": " عكسها", "probability": 0.80096435546875}, {"start": 704.17, "end": 704.59, "word": " يعني", "probability": 0.905029296875}, {"start": 704.59, "end": 705.47, "word": " موجة", "probability": 0.8115234375}, {"start": 705.47, "end": 705.65, "word": " بت", "probability": 0.66552734375}, {"start": 705.65, "end": 706.43, "word": " الشحنة", "probability": 0.894287109375}, {"start": 706.43, "end": 706.69, "word": " يعني", "probability": 0.935546875}, {"start": 706.69, "end": 707.23, "word": " basic", "probability": 0.556640625}, {"start": 707.23, "end": 707.75, "word": " step", "probability": 0.1270751953125}, {"start": 707.75, "end": 721.65, "word": " في", 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Stain و ال Acidic Stain Basic Stain are cationic يعني بيحمل شحنة موجبة when ionized the chromogen exhibits a positive charge بيحمل شحنة موجبة بما إنه بيحمل شحنة موجبة يبقى لازم يرتبط بتراكيب شحنتها سالبة", "tokens": [2407, 4587, 1211, 8315, 36145, 3224, 39894, 1863, 4587, 38251, 5296, 31598, 745, 491, 4032, 5296, 5097, 327, 299, 745, 491, 8717, 1829, 7435, 1829, 8717, 2655, 3615, 28480, 15844, 2423, 31598, 745, 491, 4032, 2423, 5097, 327, 299, 745, 491, 31598, 745, 491, 366, 3857, 313, 299, 37495, 22653, 4724, 1829, 35571, 1211, 13412, 5016, 1863, 3660, 3714, 29245, 49401, 562, 17437, 1602, 264, 16209, 8799, 39205, 257, 3353, 4602, 4724, 1829, 35571, 1211, 13412, 5016, 1863, 3660, 3714, 29245, 49401, 4724, 15042, 36145, 3224, 4724, 1829, 35571, 1211, 13412, 5016, 1863, 3660, 3714, 29245, 49401, 7251, 3555, 4587, 7578, 5296, 31377, 2304, 7251, 43500, 3555, 9566, 39894, 23557, 4117, 1829, 3555, 13412, 5016, 29399, 11296, 8608, 6027, 49401], "avg_logprob": -0.1483729309286953, 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0.60791015625}, {"start": 1122.67, "end": 1123.07, "word": " stain", "probability": 0.4501953125}, {"start": 1123.07, "end": 1123.59, "word": " ممكن", "probability": 0.9697265625}, {"start": 1123.59, "end": 1124.07, "word": " استخدم", "probability": 0.9412434895833334}, {"start": 1124.07, "end": 1124.61, "word": " مثليني", "probability": 0.6895751953125}, {"start": 1124.61, "end": 1124.77, "word": " blue", "probability": 0.7119140625}, {"start": 1124.77, "end": 1125.05, "word": " ممكن", "probability": 0.93310546875}, {"start": 1125.05, "end": 1125.49, "word": " استخدم", "probability": 0.9358723958333334}, {"start": 1125.49, "end": 1125.83, "word": " carbon", "probability": 0.324951171875}, {"start": 1125.83, "end": 1126.37, "word": " fucsin", "probability": 0.4978841145833333}, {"start": 1126.37, "end": 1127.01, "word": " ممكن", "probability": 0.9560546875}, {"start": 1127.01, "end": 1127.53, "word": " استخدم", "probability": 0.9462890625}, {"start": 1127.53, "end": 1127.95, "word": " 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\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/Ul-B8e_odMk.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/Ul-B8e_odMk.srt
new file mode 100644
index 0000000000000000000000000000000000000000..305e5b3ebe4a3c2cc5fb3c7b0a83d32d3ef7f0be
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/Ul-B8e_odMk.srt
@@ -0,0 +1,1378 @@
+1
+00:00:02,210 --> 00:00:06,090
+Nitrate reduction test. Nitrate reduction test is a
+
+2
+00:00:06,090 --> 00:00:09,010
+test to differentiate between bacteria based on
+
+3
+00:00:09,010 --> 00:00:14,030
+their ability or inability to reduce nitrate (NO₃)
+
+4
+00:00:14,030 --> 00:00:20,030
+to nitrite (NO₂) using anaerobic respiration. بقول لي
+
+5
+00:00:20,030 --> 00:00:24,630
+هذا الفحص أنا بستخدمه للتفريق بين البكتيريا وهل
+
+6
+00:00:24,630 --> 00:00:29,030
+عندها القدرة أو ما عندها القدرة أن تختزل to
+
+7
+00:00:29,030 --> 00:00:34,360
+reduce nitrate للنيترات. بدي أكشف هل البكتيريا عندها
+
+8
+00:00:34,360 --> 00:00:40,260
+القدرة أن تختزل النيترات للنيترات. يبقى النيترات
+
+9
+00:00:40,260 --> 00:00:46,040
+هي الـ substrate التي يشتغل عليها الانزيم. هيتم
+
+10
+00:00:46,040 --> 00:00:50,800
+الاختزال بواسطة انزيم نيترات ريدكتاز. من اسمه
+
+11
+00:00:50,800 --> 00:00:54,940
+نيترات ريدكتاز، يبقى هيعمل اختزال للنيترات للـ
+
+12
+00:00:54,940 --> 00:01:00,630
+substrate. هيتم اختزالها للنيترايت. يتم استخدام الـ
+
+13
+00:01:00,630 --> 00:01:04,810
+nitrate في الـ anaerobic respiration. احنا قلنا في
+
+14
+00:01:04,810 --> 00:01:08,890
+الـ anaerobic respiration، بستخدم inorganic molecule
+
+15
+00:01:08,890 --> 00:01:13,090
+كمستقبل نهائي للإلكترونات. من ضمن الـ inorganic
+
+16
+00:01:13,090 --> 00:01:17,950
+molecule كان الـ nitrate. فبالتالي بيتم استخدامه في
+
+17
+00:01:17,950 --> 00:01:22,450
+الـ anaerobic respiration. يبقى في بعض البكتيريا
+
+18
+00:01:22,450 --> 00:01:25,690
+عندها أنزيم الـ nitrate reductase. بتعمل اختزال
+
+19
+00:01:25,690 --> 00:01:31,340
+النيترات للنيترايت. Some of these bacteria بقول لي بعض
+
+20
+00:01:31,340 --> 00:01:34,980
+البكتيريا اللي عندها أنزيم النيترات reductase. both
+
+21
+00:01:34,980 --> 00:01:38,680
+of the enzymes, the two, either reduce the nitrite to
+
+22
+00:01:38,680 --> 00:01:41,660
+either ammonium or molecular nitrogen.
+
+23
+00:01:41,660 --> 00:01:45,260
+البكتيريا اللي عندها أنزيم النيترات reductase، بعد
+
+24
+00:01:45,260 --> 00:01:50,140
+ما تختزل النيترات للنيترايت، بقول لي ممكن يتم اختزال
+
+25
+00:01:50,140 --> 00:01:54,760
+النيترايت لـ ammonium ion. يبقى بعد ما يتم اختزال
+
+26
+00:01:54,760 --> 00:02:00,640
+النيترات، البكتيريا عشان تستفيد منه، ممكن إنها تحصل
+
+27
+00:02:00,640 --> 00:02:03,940
+على نيتروجين source. فبالتالي بتعمل اختزال
+
+28
+00:02:03,940 --> 00:02:09,260
+للنيترايت لـ ammonium ion. وبقول لي ممكن يتم اختزال
+
+29
+00:02:09,260 --> 00:02:13,280
+النيترايت لـ molecular nitrogen. كيف هيتم اختزال
+
+30
+00:02:13,280 --> 00:02:17,560
+النيترايت لـ molecular nitrogen؟ من خلال هذا
+
+31
+00:02:17,560 --> 00:02:23,810
+التفاعلات اللي هتصير زي ما هي موضحة في الصورة. بقول لي
+
+32
+00:02:23,810 --> 00:02:26,890
+بعد ما يتم اختزال الـ Nitrate لـ Nitrite بواسطة
+
+33
+00:02:26,890 --> 00:02:30,730
+انزيم الـ Nitrate Reductase، بيتم اختزال الـ
+
+34
+00:02:30,730 --> 00:02:34,630
+Nitrite لـ Nitric Oxide باستخدام انزيم الـ Nitrite
+
+35
+00:02:34,630 --> 00:02:39,710
+Reductase. لأنه هيتم اختزال الـ Nitrite لـ Nitric
+
+36
+00:02:39,710 --> 00:02:43,830
+Oxide، فبالتالي اسم الانزيم Nitrite Reductase. بعد
+
+37
+00:02:43,830 --> 00:02:48,170
+ما يتم اختزال الـ Nitrite لـ Nitric Oxide، الـ
+
+38
+00:02:48,170 --> 00:02:51,890
+Nitric Oxide برضه هيتم اختزاله لـ Nitrous Oxide.  إيش
+
+39
+00:02:51,890 --> 00:02:57,070
+اسم الانزيم؟ بنضيف Reductase. هيعمل اختزال للـ
+
+40
+00:02:57,070 --> 00:03:00,490
+substrate اللي قبله. هيبقى اسمه Nitric Oxide
+
+41
+00:03:00,490 --> 00:03:05,090
+Reductase. برضه الـ Nitrous Oxide، بعد ما يتم اختزاله،
+
+42
+00:03:05,090 --> 00:03:08,610
+هيتم اختزاله لنيتروجين باستخدام أنزيم الـ Nitrous
+
+43
+00:03:08,610 --> 00:03:11,770
+Oxide Reductase. لأنه بيشتغل على الـ Nitrous Oxide،
+
+44
+00:03:11,770 --> 00:03:16,030
+فبالتالي بسميه Nitrous Oxide Reductase. بالنهاية
+
+45
+00:03:16,030 --> 00:03:20,810
+هحصل على نيتروجين، على molecular nitrogen. هكذا عندي
+
+46
+00:03:20,810 --> 00:03:24,350
+أنا مصدر للـ nitrogen. بتقدر البكتيريا تنمو وتتكاثر.
+
+47
+00:03:24,350 --> 00:03:29,250
+يبقى في بعض البكتيريا عندها أنزيم الـ nitrate
+
+48
+00:03:29,250 --> 00:03:34,710
+reductase. هتعمل اختزال للنيتريت لـ nitrite. في بعض
+
+49
+00:03:34,710 --> 00:03:39,150
+البكتيريا اللي عندها هذا الانزيم، هيتم اختزال الـ
+
+50
+00:03:39,150 --> 00:03:43,950
+nitrite لـ ammonium ion. أو ممكن يصير عندي complete
+
+51
+00:03:43,950 --> 00:03:48,330
+reduction. زي ما هو موضح في الصورة، في التفاعلات
+
+52
+00:03:48,330 --> 00:03:53,180
+التالية. بالنهاية هحصل على molecular nitrogen. يبقى
+
+53
+00:03:53,180 --> 00:03:56,180
+الـ substrate التي يشتغل عليها الـ enzyme هو الـ
+
+54
+00:03:56,180 --> 00:03:59,860
+nitrate. بالنسبة لنواتج التفاعل، ممكن يكون الـ
+
+55
+00:03:59,860 --> 00:04:03,100
+nitrite. يتوقف لها فقط، يكون عندها enzyme الـ nitrate
+
+56
+00:04:03,100 --> 00:04:06,660
+reductase، ويتوقف عند الـ nitrate. ممكن يتم اختزال الـ
+
+57
+00:04:06,660 --> 00:04:09,340
+nitrate لـ ammonium ion. ناتج التفاعل يكون ammonium
+
+58
+00:04:09,340 --> 00:04:12,820
+ion. ممكن تعمل complete reduction، فبالتالي يكون
+
+59
+00:04:12,820 --> 00:04:15,360
+ناتج التفاعل molecular nitrogen.
+
+60
+00:04:21,120 --> 00:04:24,360
+Nitrate reduction test. المبدأ اللي قلنا أنه الـ
+
+61
+00:04:24,360 --> 00:04:27,000
+Nitrate هي الـ substrate التي يشتغل عليها الانزيم.
+
+62
+00:04:27,000 --> 00:04:31,000
+هكشف عن انزيم الـ Nitrate Reductase. هيشتغل على الـ
+
+63
+00:04:31,000 --> 00:04:35,180
+Nitrate. هيتم اختزاله لـ Nitrite. قلنا ممكن بعض
+
+64
+00:04:35,180 --> 00:04:40,420
+البكتيريا أنها تعمل اختزال للـ Nitrite لأمونيوم
+
+65
+00:04:40,420 --> 00:04:45,840
+عشان تحصل على نيتروجين source. برضه ممكن يتم، يتم
+
+66
+00:04:45,840 --> 00:04:50,500
+اختزال الـ Nitrate لـ molecular nitrogen. زي ما شفنا
+
+67
+00:04:50,500 --> 00:04:53,840
+في الـ slide اللي قبل. كيف سلسلة التفاعلات اللي
+
+68
+00:04:53,840 --> 00:04:58,710
+بتصير عشان أحصل بالنهاية على molecular nitrogen.
+
+69
+00:04:58,710 --> 00:05:03,190
+طبعاً في سلسلة تفاعلات، وهيتم استخدام عدة أنزيمات،
+
+70
+00:05:03,190 --> 00:05:06,090
+لكن أول أنزيم هيتم استخدامه الـ Nitrate Reductase.
+
+71
+00:05:06,090 --> 00:05:09,790
+يبقى هكشف على الـ Nitrate Reductase. في حال تم
+
+72
+00:05:09,790 --> 00:05:13,250
+اختزال الـ Nitrate لـ Molecular Nitrogen. لأنه هو
+
+73
+00:05:13,250 --> 00:05:17,370
+أول أنزيم هيشتغل على الـ Nitrate. بعدين هيحوله لـ
+
+74
+00:05:17,370 --> 00:05:20,850
+Nitrite. بعدين الـ Nitrite هيتم اختزالها باستخدام
+
+75
+00:05:20,850 --> 00:05:24,830
+أنزيم Nitrate Reductase. حتى بالنهاية أصل لـ
+
+76
+00:05:24,830 --> 00:05:28,910
+Molecular Nitrogen. يبقى سواء كان ناتج تفاعل Nitrite
+
+77
+00:05:28,910 --> 00:05:32,650
+أو ناتج تفاعل Ammonium أو molecular nitrogen، هذا
+
+78
+00:05:32,650 --> 00:05:37,730
+يعني أن البكتيريا عندها انزيم النيترات ريدكتاز. لكن
+
+79
+00:05:37,730 --> 00:05:41,290
+هنا بيصير في عندي complete reduction. هنا لأ، فقط
+
+80
+00:05:41,290 --> 00:05:46,670
+للنيترات. بعدين ممكن أنه يتم الاستفادة منه، اختزاله
+
+81
+00:05:46,670 --> 00:05:50,950
+لأمونيوم. حتى احصل على الـ nitrogen source. بالنسبة
+
+82
+00:05:50,950 --> 00:05:56,190
+للـ procedure، كيف بدي أكشف عن هذا الانزيم؟ أكيد هجيب
+
+83
+00:05:56,190 --> 00:05:59,770
+ميديا يكون فيها الـ substrate التي يشتغل عليها
+
+84
+00:05:59,770 --> 00:06:03,850
+الانزيم. الـ media اسمها Nitrate broth. بتكون الـ
+
+85
+00:06:03,850 --> 00:06:08,410
+broth. فبالتالي طريقة الزراعة: باخد inoculum، باخد
+
+86
+00:06:08,410 --> 00:06:11,090
+البكتيريا اللي بدي أكشف هل عندها الانزيم ولا لأ.
+
+87
+00:06:11,090 --> 00:06:15,270
+بعمل mix فيه الـ broth هاي، الـ Nitrate broth. بقول لي
+
+88
+00:06:15,270 --> 00:06:20,810
+بتحتوي على نصف المية KNO₃. يبقى الـ substrate التي
+
+89
+00:06:20,810 --> 00:06:24,710
+هيشتغل عليها الانزيم، الـ NO₃، بتكون موجودة في هذه
+
+90
+00:06:24,710 --> 00:06:29,130
+الميديا. بعد ما أزرع البكتيريا، هعمل incubation لمدة
+
+91
+00:06:29,130 --> 00:06:35,910
+٢٤ ساعة على ٣٧ درجة سيلزية في الـ incubator. هلأ لو
+
+92
+00:06:35,910 --> 00:06:41,470
+البكتيريا عندها أنزيم النيترات Reductase، ممكن أنه
+
+93
+00:06:41,470 --> 00:06:45,470
+يكون ناتج التفاعل Nitrite، ممكن يكون أمونيوم، ممكن
+
+94
+00:06:45,470 --> 00:06:51,250
+يكون molecular nitrogen. كيف بدي أكشف عن هدول؟ بقول
+
+95
+00:06:51,250 --> 00:06:56,820
+بتضيف بعد الـ incubation reagent. زي ما قلنا أي تفاعل،
+
+96
+00:06:56,820 --> 00:07:00,840
+كيف بدي أكشف عنه؟ إما بشوفه بعيني، أو ممكن أضيف له
+
+97
+00:07:00,840 --> 00:07:04,760
+pH indicator. إذا كان ناتج التفاعل acid أو base،
+
+98
+00:07:04,760 --> 00:07:10,120
+فبالتالي هيغير لون الوسط. أو ممكن أضيف reagent. هذا
+
+99
+00:07:10,120 --> 00:07:16,530
+الـ reagent بيتفاعل مع أحد نواتج التفاعل. تمام؟ فهنا
+
+100
+00:07:16,530 --> 00:07:20,330
+طبعاً ما عندي لا acid ولا base. مش هقدر إيش أشوف ناتج
+
+101
+00:07:20,330 --> 00:07:23,990
+التفاعل بقى. يعني ما عندي gas مثلاً. فبالتالي هضيف
+
+102
+00:07:23,990 --> 00:07:27,850
+reagent. هذا الـ reagent هيتفاعل مع أحد نواتج
+
+103
+00:07:27,850 --> 00:07:33,570
+التفاعل. بقول لي بضيف بعد الـ incubation زي فحص الـ
+
+104
+00:07:33,570 --> 00:07:37,010
+methyl red Voges-Proskauer. كنا نضيف الـ reagent بعد
+
+105
+00:07:37,010 --> 00:07:40,210
+الـ incubation. نعمل زراعة، ونضيف بعد الـ incubation
+
+106
+00:07:40,210 --> 00:07:44,670
+هذا. نفس الفكرة، نضيف بعد الـ incubation Reagent A،
+
+107
+00:07:44,670 --> 00:07:49,750
+اسمه Sulphanilic Acid. Reagent B، بضيفه Dimethyl
+
+108
+00:07:49,750 --> 00:07:54,010
+α-Naphthylamine. بضيف خمسة drops من Reagent A، وخمسة
+
+109
+00:07:54,010 --> 00:08:00,830
+drops من Reagent B، وبشوف التفاعل. بقول لي أن Reagent
+
+110
+00:08:00,830 --> 00:08:06,970
+A و Reagent B بيتفاعلوا مع الـ Nitrite. بيتفاعلوا مع
+
+111
+00:08:06,970 --> 00:08:11,490
+الـ Nitrite. قلنا أي Reagent بضيفه بيتفاعل مع نواتج
+
+112
+00:08:11,490 --> 00:08:15,420
+التفاعل. Reagent A و B بيتفاعلوا مع مين بالظبط من نواتج
+
+113
+00:08:15,420 --> 00:08:21,820
+التفاعل؟ بيتفاعلوا مع الـ nitrite. بقول لي لو الـ nitrite،
+
+114
+00:08:21,820 --> 00:08:24,560
+لو تم اختزال الـ nitrate لـ Nitrite، والـ nitrite
+
+115
+00:08:24,560 --> 00:08:29,280
+موجود، هيرتبط بـ Reagent A و B، وهيعطيني red complex.
+
+116
+00:08:29,280 --> 00:08:35,660
+فبالتالي لو اتحول لون الـ media بعد إضافة Reagent A
+
+117
+00:08:35,660 --> 00:08:39,880
+و B للون الـ red، معناه هذا أن البكتيريا عندها أنزيم
+
+118
+00:08:39,880 --> 00:08:45,010
+الـ nitrate reductase. حولت الـ nitrate لـ nitrite. يبقى
+
+119
+00:08:45,010 --> 00:08:49,070
+high positive. من الأمثلة عليها الـ E. coli. إنها حولت
+
+120
+00:08:49,070 --> 00:08:52,290
+الـ nitrate لـ nitrite. الـ nitrite اللي في الـ media
+
+121
+00:08:52,290 --> 00:08:56,030
+اتفاعل مع الـ reagent A و B اللي أنا ضفتهم، أعطاني
+
+122
+00:08:56,030 --> 00:09:01,490
+red complex. طيب لما ما يتغير اللون، بقول لي ما بقدر أكتب
+
+123
+00:09:01,490 --> 00:09:06,110
+أنه negative. ما بقدر أحكي أن هذه النتيجة negative.
+
+124
+00:09:06,110 --> 00:09:11,180
+ليش؟ لأن احنا قلنا أن ناتج التفاعل، أو البكتيريا اللي
+
+125
+00:09:11,180 --> 00:09:13,720
+عندها أنزيم الـ Nitrate Reductase، ممكن يكون ناتج
+
+126
+00:09:13,720 --> 00:09:19,020
+التفاعل Nitrite. ممكن بعد ما يتم تكون الـ Nitrite في
+
+127
+00:09:19,020 --> 00:09:23,040
+الميديا، يتم اختزاله لأمونيوم. ممكن برضه إنه يصير في
+
+128
+00:09:23,040 --> 00:09:26,880
+عندي Complete Reduction، ويتم تحويل الـ Nitrate عبر
+
+129
+00:09:26,880 --> 00:09:30,480
+سلسلة من الانزيمات، وسلسلة من التفاعلات، لـ molecular
+
+130
+00:09:30,480 --> 00:09:36,540
+nitrogen. فبالتالي لو طلع النتيجة colorless، هذا
+
+131
+00:09:36,540 --> 00:09:40,300
+لا يعني أنه negative. ممكن يكون فعلاً البكتيريا ما
+
+132
+00:09:40,300 --> 00:09:44,480
+عندها الانزيم. وممكن أن البكتيريا يكون عندها الانزيم،
+
+133
+00:09:44,480 --> 00:09:49,360
+لكن حولت النيترات لأمونيوم، أو حولت النيترات
+
+134
+00:09:49,360 --> 00:09:53,080
+لـ molecular nitrogen. في صار عندها complete
+
+135
+00:09:53,080 --> 00:09:58,180
+reduction. طيب كيف بدي أعرف هل صار في عندي complete
+
+136
+00:09:58,180 --> 00:10:02,960
+reduction، أو اتحول لأمونيوم، أو فعلاً البكتيريا ما
+
+137
+00:10:02,960 --> 00:10:06,860
+عندها انزيم النيترات الريدكتاز؟ بقول لي بضيف reagent C. في حال
+
+138
+00:10:06,860 --> 00:10:12,280
+كان اللون colorless، ما اتغير بعد
+
+139
+00:10:12,280 --> 00:10:16,880
+إضافة reagent A و B، بتضيف reagent C اللي هو الـ
+
+140
+00:10:16,880 --> 00:10:21,760
+zinc powder. الـ zinc powder يعتبر reducing agent.
+
+141
+00:10:21,760 --> 00:10:27,620
+طبعاً هضيف reagent C. بقول لي بعد إضافة reagent C، في
+
+142
+00:10:27,620 --> 00:10:32,520
+حال كانت النتيجة colorless بعد إضافة reagent A و B،
+
+143
+00:10:32,520 --> 00:10:37,680
+إذا اللون اتحول للون red، معناه هذه النتيجة negative.
+
+144
+00:10:38,400 --> 00:10:43,660
+لكن لو ما اتغير، معناه هذه النتيجة أنها positive.
+
+145
+00:10:43,660 --> 00:10:47,220
+صار عندي complete reduction. اتحول الـ nitrate لـ
+
+146
+00:10:47,220 --> 00:10:50,620
+molecular nitrogen، أو الـ nitrate بعد ما اتحول لـ
+
+147
+00:10:50,620 --> 00:10:54,420
+nitrite اتحول لـ ammonia. طيب ليش احنا قلنا أن
+
+148
+00:10:54,420 --> 00:10:58,080
+النتيجة الـ red negative، وهنقول أن النتيجة الـ
+
+149
+00:10:58,080 --> 00:11:02,800
+red positive؟ الفكرة أن الـ zinc powder يعتبر
+
+150
+00:11:02,800 --> 00:11:08,300
+reducing agent. إيش يعني reducing agent؟ يعني هيشتغل
+
+151
+00:11:08,300 --> 00:11:12,340
+شغل البكتيريا اللي عندها انزيم الـ Nitrate
+
+152
+00:11:12,340 --> 00:11:17,040
+Reductase. لو البكتيريا ما عندها الانزيم، هنا الـ
+
+153
+00:11:17,040 --> 00:11:19,620
+negative. لو ما عندها انزيم الـ Nitrate Reductase،
+
+154
+00:11:19,620 --> 00:11:24,380
+هذا يعني أنه ما صار تفاعل. النيترات، دلوقت موجودة في
+
+155
+00:11:24,380 --> 00:11:28,740
+الميديا. الـ substrate، دلت موجودة في الميديا. ما صار في
+
+156
+00:11:28,740 --> 00:11:33,020
+عندي أي تفاعل. النيترات ما اتفاعلت، دلت موجودة في
+
+157
+00:11:33,020 --> 00:11:37,640
+الميديا. هجيء الـ zinc powder اللي يعتبر reducing agent،
+
+158
+00:11:37,640 --> 00:11:42,540
+هيشتغل شغل الانزيم. هيحول الـ nitrate اللي موجودة في
+
+159
+00:11:42,540 --> 00:11:48,460
+الـ media لـ nitrite. فيها أي تفاعل لـ nitrite. الـ nitrite،
+
+160
+00:11:48,460 --> 00:11:51,360
+تمام؟ هيك صار عندي nitrite موجودة في الـ media. الـ
+
+161
+00:11:51,360 --> 00:11:55,380
+nitrite، أنا ضفت قبلها reagent A و B. سابقاً كان مضعف.
+
+162
+00:11:55,380 --> 00:12:00,530
+reagent A و B لما صار colorless. هيتفاعلوا، reagent
+
+163
+00:12:00,530 --> 00:12:06,590
+A و B مع الـ nitrite اللي تم اختزاله بواسطة الـ
+
+164
+00:12:06,590 --> 00:12:11,810
+zinc powder، وليس بواسطة البكتيريا. لأنه ليش تم
+
+165
+00:12:11,810 --> 00:12:18,050
+تحويله؟ لأن الـ nitrate لم يتم استهلاكه. كانت
+
+166
+00:12:18,050 --> 00:12:20,850
+البكتيريا معاها أنزيم الـ nitrate reductase. فضلت
+
+167
+00:12:20,850 --> 00:12:24,010
+موجودة في الـ media. فبالتالي هيجئ الـ zinc powder،
+
+168
+00:12:24,010 --> 00:12:28,380
+هيعملها reduction للـ nitrite. هيتفاعل مع reagent A
+
+169
+00:12:28,380 --> 00:12:32,340
+و B الموجود سابقاً. فهيتحول اللون للون Red. فبالتالي
+
+170
+00:12:32,340 --> 00:12:36,640
+اللون الـ Red بعد إضافة reagent C اللي هو الـ zinc
+
+171
+00:12:36,640 --> 00:12:40,880
+powder يعتبر negative. من الأمثلة على البكتيريا، I
+
+172
+00:12:40,880 --> 00:12:44,080
+اللي ما عندها الانزيم النيترات ريدكتاز اللي هو
+
+173
+00:12:44,080 --> 00:12:51,
+
+223
+00:16:10,460 --> 00:16:15,360
+Zinc Powder وليس بسبب البكتيريا أو الإنزيم الموجود
+
+224
+00:16:15,360 --> 00:16:21,390
+في البكتيريا، نمر على الـ slide بسرعة، organism
+
+225
+00:16:21,390 --> 00:16:24,850
+that possesses the enzyme nitrate reductase، البكتيريا
+
+226
+00:16:24,850 --> 00:16:28,290
+اللي عندها أنزيم النيترات ريدكتاز ستعمل اختزالًا من
+
+227
+00:16:28,290 --> 00:16:32,550
+النيترات إلى نيترايت، قلنا النيترات هو الـ substrate
+
+228
+00:16:32,550 --> 00:16:36,310
+لأنزيم النيترات ريدكتاز.  بقول: النيترات، إذا
+
+229
+00:16:36,310 --> 00:16:39,830
+ملاحظين، هي النيترات. كيف بدي أكشف عنه؟ قلنا أنّه
+
+230
+00:16:39,830 --> 00:16:44,200
+reagent A و B سيتفاعلان فقط مع النيترات، ومع ناتج
+
+231
+00:16:44,200 --> 00:16:47,100
+التفاعل النيترات، وليس مع الـ ammonium، وليس مع الـ
+
+232
+00:16:47,100 --> 00:16:51,780
+molecular nitrogen. فبالتالي، لو كانت النتيجة لم
+
+233
+00:16:51,780 --> 00:16:55,640
+يتغير لونها، هذا لا يعني أنّه لا عندها الإنزيم، ممكن
+
+234
+00:16:55,640 --> 00:16:59,290
+أنّها تكون عاملة complete reduction،  مش موجود، أندي
+
+235
+00:16:59,290 --> 00:17:03,630
+ناتج التفاعل نيتريت، موجود، موليكولر نيتروجين، أو
+
+236
+00:17:03,630 --> 00:17:08,250
+أمونيوم. فبالتالي، لم يتفاعلا مع reagent A و B. فهضيف
+
+237
+00:17:08,250 --> 00:17:11,590
+الذي هو الـ zinc powder. كيف بدي أكشف على الـ nitrite
+
+238
+00:17:11,590 --> 00:17:16,250
+ion؟ بقول: بإضافة sulfanilic acid (reagent A). ما
+
+239
+00:17:16,250 --> 00:17:20,620
+ذكرناه، الذي هو يُعتبر اسمه salphanilic acid (reagent
+
+240
+00:17:20,620 --> 00:17:24,980
+A) باسمه الـ dimethyl naphthalamide. يبقى reagent A
+
+241
+00:17:24,980 --> 00:17:28,360
+(salphanilic acid)، reagent B (dimethyl naphthalamide).
+
+242
+00:17:28,360 --> 00:17:32,800
+سأضيفهما على المزرعة بعد الـ incubation 24 ساعة.
+
+243
+00:17:32,800 --> 00:17:37,340
+بقول لي: any nitrite in the media، أي نيتريت موجود في
+
+244
+00:17:37,340 --> 00:17:40,520
+الـ media سيتفاعل مع هذه الـ reagents وسيعطيني red
+
+245
+00:17:40,520 --> 00:17:44,520
+color. يبقى بعد إضافة reagent A و B، إذا تحول اللون
+
+246
+00:17:44,520 --> 00:17:49,620
+إلى اللون الأحمر، هذا يعني أنّه تم تحويل الـ Nitrate إلى
+
+247
+00:17:49,620 --> 00:17:54,360
+Nitrite باستخدام إنزيم الـ Nitrate Reductase. هذه
+
+248
+00:17:54,360 --> 00:17:57,860
+الصورة للتفاعل. قلنا الـ Nitrate هو الـ substrate، هو
+
+249
+00:17:57,860 --> 00:18:01,260
+تم اختزاله إلى Nitrite. ممكن الـ Nitrate، بعض أنواع
+
+250
+00:18:01,260 --> 00:18:05,140
+البكتيريا، تعمله اختزالًا إلى Ammonium، وممكن يصير في
+
+251
+00:18:05,140 --> 00:18:08,410
+عندي complete reduction،  أحصل على molecular
+
+252
+00:18:08,410 --> 00:18:11,970
+nitrogen. يعني في النهاية سأحصل على nitrogen source.
+
+253
+00:18:11,970 --> 00:18:16,830
+لازم اللي هو البكتيريا. بقول لي: لو البكتيريا لم يتغير
+
+254
+00:18:16,830 --> 00:18:21,970
+لونها،  أين هو التغيير في اللون بعد إضافة
+
+255
+00:18:21,970 --> 00:18:26,230
+reagent A و B؟ هذا يعني أن البكتيريا The bacteria
+
+256
+00:18:26,230 --> 00:18:29,350
+possesses nitrate reductase، عندها البكتيريا نيترات
+
+257
+00:18:29,350 --> 00:18:34,630
+ريدكتاز، لكنّها عملت اختزالًا للنيترات إلى أمونيوم. قلنا
+
+258
+00:18:34,630 --> 00:18:37,810
+ممكن بعد ما يتم اختزال النيترات إلى نيترايت، يتم
+
+259
+00:18:37,810 --> 00:18:42,070
+اختزاله إلى أمونيوم، أو ممكن أن يتم اختزاله إلى
+
+260
+00:18:42,070 --> 00:18:46,480
+molecular نيتروجين، في سلسلة من التفاعلات. هذه نفس
+
+261
+00:18:46,480 --> 00:18:49,760
+الفكرة، أنّه عندها الإنزيم، لكنّها عملت اختزالًا للنيترات إلى
+
+262
+00:18:49,760 --> 00:18:54,620
+أمونيوم، أو الاحتمال الثالث أنّه البكتيريا لا عندها
+
+263
+00:18:54,620 --> 00:18:58,480
+إنزيم النيترات Reductase، فبالتالي لم يصير اختزال
+
+264
+00:18:58,480 --> 00:19:05,350
+للنيترات. بقول لي:  كي أحدد هل البكتيريا عندها
+
+265
+00:19:05,350 --> 00:19:09,850
+ال إنزيم، عملت complete reduction، أو حولته إلى أمونيوم،
+
+266
+00:19:09,850 --> 00:19:13,810
+أو فعلاً هي لا عندها الإنزيم، سأضيف الـ zinc powder، a
+
+267
+00:19:13,810 --> 00:19:16,190
+small amount of zinc powder is added to the
+
+268
+00:19:16,190 --> 00:19:20,050
+culture containing the reagents. سأضيف الـ zinc
+
+269
+00:19:20,050 --> 00:19:24,810
+powder للمزرعة التي تحتوي على reagent A وB سابقًا.
+
+270
+00:19:25,450 --> 00:19:29,050
+بقول لي: الـ Zinc، ما وظيفته؟ الـ Zinc Powder يُعتبر
+
+271
+00:19:29,050 --> 00:19:32,710
+قلنا: الـ Reducing Agent. سيختزل النيترات والنيترايت.
+
+272
+00:19:32,710 --> 00:19:36,730
+قلنا: سيعمل اختزالًا للنترايت الموجود في الميديا
+
+273
+00:19:36,730 --> 00:19:42,250
+إلى نيترات، فبالتالي، a pink or red color سيظهر، سيتحول
+
+274
+00:19:42,250 --> 00:19:46,350
+لون الوسط إلى اللون الأحمر، لأن تم اختزال النيترات
+
+275
+00:19:46,350 --> 00:19:49,890
+إلى نيترايت بواسطة الـ Zinc Powder، وليس بواسطة
+
+276
+00:19:49,890 --> 00:19:53,510
+البكتيريا. الـ Nitrite موجود في الميديا. قلنا الـ
+
+277
+00:19:53,510 --> 00:19:57,140
+Nitrite يرتبط بـ reagent A و B الموجودين في الميديا
+
+278
+00:19:57,140 --> 00:20:01,160
+فيعطيني اللون الأحمر. بقول لي: الحقيقة أنّ الـ Nitrate
+
+279
+00:20:01,160 --> 00:20:05,280
+were not reduced to nitrite by the bacteria. في هذه
+
+280
+00:20:05,280 --> 00:20:08,860
+الحالة، أنّ الـNitrate لم يتم اختزاله إلى Nitrite
+
+281
+00:20:08,860 --> 00:20:12,300
+باستخدام البكتيريا، تم اختزاله باستخدام الـ Zinc
+
+282
+00:20:12,300 --> 00:20:16,800
+powder. طيب، لماذا البكتيريا؟ لماذا الـ Zinc powder قدر
+
+283
+00:20:16,800 --> 00:20:19,460
+أن يختزل من الـ Nitrate إلى Nitrite؟ بقول لي: لأنّ
+
+284
+00:20:19,460 --> 00:20:24,830
+الـNitrate لم يتم استهلاكه، Nitrate unreacted، لم يتم
+
+285
+00:20:24,830 --> 00:20:30,050
+استهلاكه، فهذا يعني أنّ البكتيريا لا عندها إنزيم الـ
+
+286
+00:20:30,050 --> 00:20:34,910
+nitrate reductase. لكن الـ red color doesn't appear،
+
+287
+00:20:34,910 --> 00:20:38,730
+إذا لم يظهر اللون الأحمر، هذا يعني أنّ الـ nitrate
+
+288
+00:20:38,730 --> 00:20:42,810
+الموجود في الـ media تم اختزاله سابقًا إلى nitrite،
+
+289
+00:20:43,250 --> 00:20:46,750
+بعدها تم اختزاله إلى ammonium or nitrogen gas.
+
+290
+00:20:46,750 --> 00:20:50,690
+فبالتالي، هنا، أنّ الـ nitrate، الـ substrate، تم
+
+291
+00:20:50,690 --> 00:20:54,810
+استهلاكه. فلما يأتي الـ zinc powder، سيعمل عليه، مش
+
+292
+00:20:54,810 --> 00:20:57,690
+سيجد،  لن يجد شيئًا يعمل عليه، فبالتالي لن يصير
+
+293
+00:20:57,690 --> 00:21:01,330
+أي تفاعل، لن يصير أي تغيير في اللون. فهذا يعني أنّ
+
+294
+00:21:01,330 --> 00:21:05,090
+صار في عندي complete reduction، أو تحول الـ nitrate،
+
+295
+00:21:05,090 --> 00:21:10,810
+تم اختزاله إلى ammonium. كيف الـ procedure؟ قلنا نستخدم
+
+296
+00:21:10,810 --> 00:21:14,470
+نيترات،  الـ broth فيها بوتاسيوم نيترات، نصف المية.
+
+297
+00:21:14,470 --> 00:21:19,010
+أعمل incubation، سأضيف خمسة drops من reactant A، وخمسة
+
+298
+00:21:19,010 --> 00:21:24,070
+drops من reactant B. بقول لي: if nitrate is present in
+
+299
+00:21:24,070 --> 00:21:27,850
+the media، it will turn red within one to two
+
+300
+00:21:27,850 --> 00:21:33,090
+minutes. يعني أنّ في خطأ في الـ slide، نغيّرها إلى
+
+301
+00:21:33,090 --> 00:21:37,420
+nitrite. لو الـ Nitrite موجودة في الميديا، قلنا
+
+302
+00:21:37,420 --> 00:21:40,760
+ستتفاعل مع reagent A و B، وستعطيني اللون الأحمر.
+
+303
+00:21:40,760 --> 00:21:45,000
+يبقى الـ positive هو red color بعد إضافة reagent A
+
+304
+00:21:45,000 --> 00:21:52,060
+و B. لو لم يتغير، قلنا لا نحكي negative، لا نحكي
+
+305
+00:21:52,060 --> 00:21:56,040
+negative. الـ negative test، بعد إضافة reagent A و B،
+
+306
+00:21:56,040 --> 00:22:00,840
+قلنا: لا نضيف zinc powder. لو تحوّل اللون إلى اللون الأحمر،
+
+307
+00:22:00,840 --> 00:22:05,380
+هذا يعني أنّ النتيجة negative، لأنّ الـ zinc powder اشتغل
+
+308
+00:22:05,380 --> 00:22:08,900
+على الـ Nitrate الذي لم يتم استهلاكه، لأنّ البكتيريا
+
+309
+00:22:08,900 --> 00:22:14,040
+لا عندها إنزيم الـ Nitrate Reductase. لكنّ النتيجة
+
+310
+00:22:14,040 --> 00:22:16,940
+الـ positive تعني أنّ البكتيريا عملت Complete
+
+311
+00:22:16,940 --> 00:22:20,860
+Reduction، أو تم اختزال الـ Nitrate إلى أمونيوم.
+
+312
+00:22:20,860 --> 00:22:24,260
+فبالتالي، جاء الـ zinc powder، سيعمل على الـ Nitrate، ما
+
+313
+00:22:24,260 --> 00:22:28,520
+وجد الـNitrate، تم استهلاكه، فبالتالي لن يصير تفاعل،
+
+314
+00:22:28,520 --> 00:22:33,450
+سيكون Colorless. يبقى سنعدّل: إن لم يكن الـ Nitrate، لو
+
+315
+00:22:33,450 --> 00:22:36,590
+الـ Nitrate موجود في الـ media، سيتحول اللون إلى اللون
+
+316
+00:22:36,590 --> 00:22:43,730
+أحمر، لأنّه هو الذي سيرتبط بـ reagent A و B. هنا
+
+317
+00:22:43,730 --> 00:22:46,550
+التفاعل. قلنا في هنا أكثر من احتمال، ممكن
+
+318
+00:22:46,550 --> 00:22:50,430
+البكتيريا تحوّل الـ Nitrate إلى Nitrite، ممكن يتم
+
+319
+00:22:50,430 --> 00:22:54,630
+تحويل الـ Nitrate إلى molecular nitrogen، لأنّها تعمل
+
+320
+00:22:54,630 --> 00:22:58,450
+complete reduction. قلنا: ممكن بعد ما يتم اختزال الـ
+
+321
+00:22:58,450 --> 00:23:03,080
+Nitrate إلى Nitrite، أنّها تختزل الـ Nitrate إلى Ammonia.
+
+322
+00:23:03,080 --> 00:23:06,800
+يعني يكون التفاعل: اختزال الـ Nitrate إلى Ammonia.
+
+323
+00:23:06,800 --> 00:23:09,220
+ممكن لا يصير في نتيجة، الـ negative. هذه التفاعلات، هذه
+
+324
+00:23:09,220 --> 00:23:15,100
+النتيجة الـ positive، هذا يعني أنّ عندها الإنزيم،
+
+325
+00:23:15,100 --> 00:23:21,000
+لكنّ نتائج التفاعل تختلف. هنا نتيجة التفاعل: Nitrate،
+
+326
+00:23:21,000 --> 00:23:26,840
+هنا نيتروجين غاز، هنا Ammonia.  الرابع: واحدة هي لا
+
+327
+00:23:26,840 --> 00:23:31,250
+عندها إنزيم. يبقى نقطة: هذه هي الـ positive.
+
+328
+00:24:21,150 --> 00:24:23,390
+لكن هذه آخر issue، هي negative.
+
+329
+00:24:47,360 --> 00:24:51,440
+يبقى سواء كان ناتج التفاعل نيتريت، أو molecular
+
+330
+00:24:51,440 --> 00:24:54,680
+nitrogen، أو أمونيوم، هذا يعني أنّ البكتيريا عندها
+
+331
+00:24:54,680 --> 00:24:59,300
+ال إنزيم. لكنّ هنا يصير عندي complete reduction، لو
+
+332
+00:24:59,300 --> 00:25:03,660
+لم يصِر تفاعل، يبقى، أو لا عندها البكتيريا الإنزيم، يبقى
+
+333
+00:25:03,660 --> 00:25:07,140
+لن يصير تفاعل. فبالتالي، النيترات، الـ substrate، ستظل
+
+334
+00:25:07,140 --> 00:25:11,340
+موجودة في الـ media. أكيد بالنسبة لـ nitrogen gas،
+
+335
+00:25:11,340 --> 00:25:15,320
+لا شيء موجود، لكن في حال كانت البكتيريا عملت
+
+336
+00:25:15,320 --> 00:25:18,460
+complete reduction، حولت النيترات إلى molecular
+
+337
+00:25:18,460 --> 00:25:23,960
+nitrogen، في عندي سيكون nitrogen gas. بعد ما أضيف
+
+338
+00:25:23,960 --> 00:25:28,580
+reagent A و B، يقول لي: إذا تحول اللون إلى اللون الأحمر، هذا
+
+339
+00:25:28,580 --> 00:25:34,300
+يعني أنّ البكتيريا حولت النيترات إلى نيترايت فقط. لماذا؟
+
+340
+00:25:34,300 --> 00:25:39,420
+لأنّ قلنا إنّ الـreagent A وB يتفاعلان مع الـnitrite،
+
+341
+00:25:39,420 --> 00:25:43,400
+لا يتفاعلان مع الـnitrogen، molecular nitrogen، أو
+
+342
+00:25:43,400 --> 00:25:46,620
+الـammonium، أو الـnitrate. في حال كانت البكتيريا
+
+343
+00:25:46,620 --> 00:25:49,840
+negative، يبقى بعد إضافة reagent A وB، إذا تحوّل
+
+344
+00:25:49,840 --> 00:25:54,680
+اللون إلى اللون الأحمر، نرى التفاعل، إذا ناتج التفاعل
+
+345
+00:25:54,680 --> 00:25:58,760
+Nitrite، يبقى نحكي أنّ في عندي، سيتحول اللون إلى اللون
+
+346
+00:25:58,760 --> 00:26:01,940
+الأحمر. لكن لو كان ناتج التفاعل ليس Nitrite، يبقى كلّهم
+
+347
+00:26:01,940 --> 00:26:05,350
+هنا، ناتج التفاعل نيتريت، ليس ناتج التفاعل نيتريت،
+
+348
+00:26:05,350 --> 00:26:11,530
+ليس عندي تفاعل. المواد المتفاعلة: نيتريت، ليس نيتريت،
+
+349
+00:26:11,530 --> 00:26:14,750
+يبقى لن يصير تغيير في اللون، لأنّ الـ reagent A و B
+
+350
+00:26:14,750 --> 00:26:18,510
+يرتبطان مع الـ نيتريت فقط. في حال كان ناتج التفاعل
+
+351
+00:26:18,510 --> 00:26:24,090
+نيتريت، هو هنا، سيُحول اللون إلى اللون الأحمر. طيب،
+
+352
+00:26:24,090 --> 00:26:28,250
+بقول لي: بعد إضافة الـ zinc powder، أكيد في حال كان
+
+353
+00:26:28,250 --> 00:26:32,500
+البكتيريا حولت الـ نيترات إلى نيترايت. لا أضيف الـ zinc
+
+354
+00:26:32,500 --> 00:26:36,420
+powder، فقط أضيف الـ zinc powder في حال لم يتغير اللون
+
+355
+00:26:36,420 --> 00:26:40,080
+بعد إضافة reagent A وB. في هذه الحالة، لا أضيف الـ zinc
+
+356
+00:26:40,080 --> 00:26:43,860
+powder، أعتبرها positive على طول. لكن في حال لم يتغير
+
+357
+00:26:43,860 --> 00:26:47,340
+اللون، أضيف الـ zinc powder كي أفرّق بين البكتيريا التي
+
+358
+00:26:47,340 --> 00:26:50,020
+لا عندها الـ enzyme، وبين البكتيريا التي عملت
+
+359
+00:26:50,020 --> 00:26:54,110
+complete reduction. بعد إضافة الزنك powder، إذا تحوّل
+
+360
+00:26:54,110 --> 00:26:58,190
+اللون إلى اللون pink to red، هذا يعني أنّ البكتيريا لا
+
+361
+00:26:58,190 --> 00:27:02,430
+عندها الإنزيم، لأنّ قلنا zinc powder يعمل على  ما بقي،
+
+362
+00:27:02,430 --> 00:27:07,430
+يعمل على النيترات. فإن رأيت نيترات، سيتحول
+
+363
+00:27:07,430 --> 00:27:11,090
+اللون إلى اللون الأحمر. لكن هنا، في حال كانت عملت complete
+
+364
+00:27:11,090 --> 00:27:14,350
+reduction، أو في حال تحول النيترات إلى أمونيوم، أكيد
+
+365
+00:27:14,350 --> 00:27:20,050
+بعد إضافة zinc powder لن يتغير اللون عندي، لأنّه لن
+
+366
+00:27:20,050 --> 00:27:23,730
+يعمل، ليس موجود الـ substrate الذي هو النيترات
+
+367
+00:27:23,730 --> 00:27:26,970
+الذي سيعمل عليه. فبالتالي، لن يصير تفاعل. لكن في
+
+368
+00:27:26,970 --> 00:27:30,970
+حالة الـ negative، أكيد الـ nitrate لم يتم استهلاكه،
+
+369
+00:27:30,970 --> 00:27:34,530
+سيظلّ موجودًا في الـ media. سيأتي الـ zinc powder،
+
+370
+00:27:34,530 --> 00:27:38,010
+الذي سيعمل عمل الإنزيم، سيحوّلها إلى Nitrite، الـ
+
+371
+00:27:38,010 --> 00:27:41,930
+nitrite سيرتبط مع الـ reagent A و B التي أضفتها سابقًا،
+
+372
+00:27:41,930 --> 00:27:43,630
+فسيتحول اللون إلى اللون الأحمر.
+
+373
+00:27:50,080 --> 00:27:55,620
+هذه الصورة، في الميديا فيها نيترات. أضفت reagent A وB،
+
+374
+00:27:55,620 --> 00:28:00,540
+لم يتغير اللون.  ففي هذه الساعة، سأضيف الـ zinc powder. بعد
+
+375
+00:28:00,540 --> 00:28:04,180
+إضافة الـ zinc powder، إذا تحوّل اللون إلى اللون الأحمر، هذا
+
+376
+00:28:04,180 --> 00:28:12,060
+يعني أنّ النتيجة negative. أضفت reagent، هذه نفس
+
+377
+00:28:12,060 --> 00:28:16,340
+الفكرة، لم يتغير اللون. ففي هذه الساعة سأضيف reagent C، reagent
+
+378
+00:28:16,340 --> 00:28:22,980
+C، إذا بعد إضافة reagent C، إذا تحوّل اللون إلى اللون الأحمر،
+
+379
+00:28:22,980 --> 00:28:27,720
+يبقى هذا يعني أنّ البكتيريا لا عندها الإنزيم. هذه
+
+380
+00:28:27,720 --> 00:28:32,980
+النتيجة الـ negative. هذه النتيجة أيضًا. ملخّص: أضفت
+
+381
+00:28:32,980 --> 00:28:39,800
+زراعة بكتيريا في الـ media، في الـ nitrate، أضفت
+
+382
+00:28:39,800 --> 00:28:43,880
+reagent A و reagent B. بقول لي: لو أضفت reagent A و B، و
+
+383
+00:28:43,880 --> 00:28:47,760
+تحوّل اللون إلى اللون الأحمر، هذا يعني أنّ النتيجة positive.
+
+384
+00:28:48,610 --> 00:28:52,390
+لكن إذا لم يتغير اللون، سأضيف الـ zinc powder. إذا بعد
+
+385
+00:28:52,390 --> 00:28:56,070
+إضافة الـ zinc powder لم يتغير اللون، هذا يعني أنّها
+
+386
+00:28:56,070 --> 00:28:59,410
+عملت complete reduction، أو تحوّل الـ nitrate إلى
+
+387
+00:28:59,410 --> 00:29:03,790
+ammonia. يبقى هذه في حال كانت positive. لكنّ الصورة
+
+388
+00:29:03,790 --> 00:29:11,210
+الأُخرى في حال كان negative. هي نفس الفكرة. بقول لي أنّ
+
+389
+00:29:11,210 --> 00:29:18,170
+طبعًا بعد ما أضفت الـ reagent A وB، هنا أضفت أول شيء
+
+390
+00:29:18,170 --> 00:29:21,690
+طبعًا زراعتي البكتيريا، أضفت reagent A و
+
+445
+00:33:08,210 --> 00:33:11,990
+هيك من جون خلصنا الفحص الـ Nitrate Reduction Test
+
+446
+00:33:11,990 --> 00:33:17,930
+وخلصنا شعب ترب التلات فحوصات الـ Ureas و الـ Indole و
+
+447
+00:33:17,930 --> 00:33:21,410
+الـ Nitrate Reduction Test السلام عليكم ورحمة الله
+
+448
+00:33:21,410 --> 00:33:22,210
+وبركاته
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/Ul-B8e_odMk_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/Ul-B8e_odMk_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..e9ce96b740a69f542dcb575aa369b42b0160a2e2
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/Ul-B8e_odMk_postprocess.srt
@@ -0,0 +1,1792 @@
+1
+00:00:02,210 --> 00:00:06,090
+Nitrate reduction test Nitrate reduction test is a
+
+2
+00:00:06,090 --> 00:00:09,010
+test to differentiate between bacteria based on
+
+3
+00:00:09,010 --> 00:00:14,030
+their ability or inability to reduce nitrate NO3
+
+4
+00:00:14,030 --> 00:00:20,030
+to nitrite NO2 using anaerobic respiration بقوللي
+
+5
+00:00:20,030 --> 00:00:24,630
+هذا الفحص أنا بستخدمه للتفريق بين البكتيريا وهل
+
+6
+00:00:24,630 --> 00:00:29,030
+عندها القدرة او ما عندها القدرة انها تختزل to
+
+7
+00:00:29,030 --> 00:00:34,360
+reduce nitrateللنيترات بدي اكشفها للبكتيريا عندها
+
+8
+00:00:34,360 --> 00:00:40,260
+القدرة انها تختزل النيترات لنيترات يبقى النيترات
+
+9
+00:00:40,260 --> 00:00:46,040
+هي ال substrate اللى هى يشتغل عليها الانزيم هيتم
+
+10
+00:00:46,040 --> 00:00:50,800
+الاختزال بواسطة انزيم نيترات ريدكتاز من اسمه
+
+11
+00:00:50,800 --> 00:00:54,940
+نيترات ريدكتاز يبقى هيعمل اختزال للنيترات لل
+
+12
+00:00:54,940 --> 00:01:00,630
+substrate هيتم اختزالها لنيتراتهيتم استخدام الـ
+
+13
+00:01:00,630 --> 00:01:04,810
+nitrate في الـ anaerobic respiration احنا قلنا في
+
+14
+00:01:04,810 --> 00:01:08,890
+ال anaerobic respiration بستخدم inorganic molecule
+
+15
+00:01:08,890 --> 00:01:13,090
+كمستقبل لنهائي للإلكترونات من ضمن ال inorganic
+
+16
+00:01:13,090 --> 00:01:17,950
+molecule كان ال nitrate فبالتالي بيتم استخدامه في
+
+17
+00:01:17,950 --> 00:01:22,450
+ال anaerobic respiration يبقى في بعض البكتيريا
+
+18
+00:01:22,450 --> 00:01:25,690
+عندها أنزيم ال nitrate reductase بتعمل اختزال
+
+19
+00:01:25,690 --> 00:01:31,340
+لنيترات لنيتراتsome of these bacteria بقولي بعض
+
+20
+00:01:31,340 --> 00:01:34,980
+البكتيريا اللي عندها أنزيم النيترات reductase both
+
+21
+00:01:34,980 --> 00:01:38,680
+of the enzyme the two either reduce the nitrite to
+
+22
+00:01:38,680 --> 00:01:41,660
+either the ammonium or molecular nitrogen
+
+23
+00:01:41,660 --> 00:01:45,260
+البكتيريا اللي عندها أنزيم النيترات reductase بعد
+
+24
+00:01:45,260 --> 00:01:50,140
+ما تختزل النيترات لنيترات بقولي ممكن يتم اختزال
+
+25
+00:01:50,140 --> 00:01:54,760
+النيترات ل ammonium ion يبقى بعد ما يتم اختزال
+
+26
+00:01:54,760 --> 00:02:00,640
+النيترات البكتيريا عشان تستفيد منهممكن إنها تحصل
+
+27
+00:02:00,640 --> 00:02:03,940
+على نيتروجين source فبالتالي بتعمل اختزال
+
+28
+00:02:03,940 --> 00:02:09,260
+للنيترائت ل ammonium ion و بقول لي ممكن يتم اختزال
+
+29
+00:02:09,260 --> 00:02:13,280
+النيترائت ل molecular nitrogen كيف هيتم اختزال
+
+30
+00:02:13,280 --> 00:02:17,560
+النيترائت ل molecular nitrogen من خلال هذا
+
+31
+00:02:17,560 --> 00:02:23,810
+التفاعلات اللي هتصير زي ما هي موضحة في الصورةبقولي
+
+32
+00:02:23,810 --> 00:02:26,890
+بعد ما يتم اختزال الـ Nitrate لـ Nitrite بواسط
+
+33
+00:02:26,890 --> 00:02:30,730
+انزيم الـ Nitrate Reductase بيتم اختزال الـ
+
+34
+00:02:30,730 --> 00:02:34,630
+Nitrite لـ Nitric Oxide باستخدام انزيم الـ Nitrate
+
+35
+00:02:34,630 --> 00:02:39,710
+Reductase لأنه هيتم اختزال الـ Nitrite لـ Nitric
+
+36
+00:02:39,710 --> 00:02:43,830
+Oxide فبالتالي اسم الانزيم Nitrate Reductase بعد
+
+37
+00:02:43,830 --> 00:02:48,170
+ما يتم اختزال الـ Nitrite لـ Nitric Oxide الـ
+
+38
+00:02:48,170 --> 00:02:51,890
+Nitric Oxide برضه هيتم اختزاله لـ Nitrous Oxide اش
+
+39
+00:02:51,890 --> 00:02:57,070
+اسم الانزيم؟بنضيف Redactase هيعمل اختزال ل ال
+
+40
+00:02:57,070 --> 00:03:00,490
+substrate اللى قبل هيبقى اسمه Nitric Oxide
+
+41
+00:03:00,490 --> 00:03:05,090
+Redactase برضه ال Nitrous Oxide بعد ما يتم اختزاله
+
+42
+00:03:05,090 --> 00:03:08,610
+هيتم اختزاله لنيتروجين باستخدام أنزيم ال Nitrous
+
+43
+00:03:08,610 --> 00:03:11,770
+Oxide Redactase لأنه شتغل على ال Nitrous Oxide
+
+44
+00:03:11,770 --> 00:03:16,030
+فبالتالي بسميه Nitrous Oxide Redactase بالنهاية
+
+45
+00:03:16,030 --> 00:03:20,810
+هحصل على نيتروجين على molecular nitrogenهك عندي
+
+46
+00:03:20,810 --> 00:03:24,350
+أنا مصدر للـ nitrogen بتقدر البكتيريا تنمو وتتكثر
+
+47
+00:03:24,350 --> 00:03:29,250
+يبقى في بعض البكتيريا عندها أنزيم الـ nitrate
+
+48
+00:03:29,250 --> 00:03:34,710
+reductase هتعمل اختزال للنيتريات لـ nitrite في بعض
+
+49
+00:03:34,710 --> 00:03:39,150
+البكتيريا اللي عندها هذا الانزيم هيتم اختزال الـ
+
+50
+00:03:39,150 --> 00:03:43,950
+nitrite لـ ammonium ion أو ممكن يصير عندي complete
+
+51
+00:03:43,950 --> 00:03:48,330
+reduction زي ما هو موضح في الصورة في التفاعلات
+
+52
+00:03:48,330 --> 00:03:53,180
+التاليةبالنهاية هحصل على molecular nitrogen يبقى
+
+53
+00:03:53,180 --> 00:03:56,180
+ال substrate اللى هيشتغل عليها ال enzyme هو ال
+
+54
+00:03:56,180 --> 00:03:59,860
+nitrate بالنسبة للنواتج التفاعل ممكن يكون ال
+
+55
+00:03:59,860 --> 00:04:03,100
+nitrate اتوقف لها فقط يكون عندها enzyme ال nitrate
+
+56
+00:04:03,100 --> 00:04:06,660
+تتردكتز وتتوقف لل nitrate ممكن يتم اختزال ال
+
+57
+00:04:06,660 --> 00:04:09,340
+nitrate ل ammonium ion ناتج التفاعل يكون ammonium
+
+58
+00:04:09,340 --> 00:04:12,820
+ion ممكن تعمل complete reduction فبالتالي يكون
+
+59
+00:04:12,820 --> 00:04:15,360
+ناتج التفاعل molecular nitrogen
+
+60
+00:04:21,120 --> 00:04:24,360
+Nitrate reduction test لبرنسيب اللي قلنا انه ال
+
+61
+00:04:24,360 --> 00:04:27,000
+Nitrate هي ال substrate اللي هيشتغل عليها الانزيم
+
+62
+00:04:27,000 --> 00:04:31,000
+هكشف عن انزيم ال Nitrate Reductase هيشتغل على ال
+
+63
+00:04:31,000 --> 00:04:35,180
+Nitrate هيتم اختزاله ل Nitrite قلنا ممكن بعض
+
+64
+00:04:35,180 --> 00:04:40,420
+البكتيريا انها تعمل اختزال لل Nitrite لأمونيوم
+
+65
+00:04:40,420 --> 00:04:45,840
+عشان تحصل على نيتروجين source برضه ممكن يتم يتم
+
+66
+00:04:45,840 --> 00:04:50,500
+اختزال ال Nitrate لموليكولار نيتروجين زي ما شفنا
+
+67
+00:04:50,500 --> 00:04:53,840
+في ال slide اللي قبل كيف سلسلة التفاعلات اللي
+
+68
+00:04:53,840 --> 00:04:58,710
+بتصير عشان أحصل بالنهاية على موليكولارالنيتروجين
+
+69
+00:04:58,710 --> 00:05:03,190
+طبعا في سلسلة تفاعلات وهيتم استخدام عدة أنزيمات
+
+70
+00:05:03,190 --> 00:05:06,090
+لكن أول أنزيم هيتم استخدامه الـ Nitrate Reductase
+
+71
+00:05:06,090 --> 00:05:09,790
+يبقى هكشف على الـ Nitrate Reductase في حال تم
+
+72
+00:05:09,790 --> 00:05:13,250
+اختزال الـ Nitrate لـ Molecular Nitrogen لأن هو
+
+73
+00:05:13,250 --> 00:05:17,370
+أول أنزيم هيشتغل على الـ Nitrate بعدين هيحوله لـ
+
+74
+00:05:17,370 --> 00:05:20,850
+Nitrate بعدين الـ Nitrate هيتم اختزالها باستخدام
+
+75
+00:05:20,850 --> 00:05:24,830
+أنزيم Nitrate Reductase حتى بالنهاية أصل لـ
+
+76
+00:05:24,830 --> 00:05:28,910
+Molecular Nitrogenيبقى سواء كان ناتج تفاعل نيترات
+
+77
+00:05:28,910 --> 00:05:32,650
+او ناتج تفاعل امونيوم او molecular nitrogen هذا
+
+78
+00:05:32,650 --> 00:05:37,730
+يعني ان البكتيريا عندها انزيم النيترات ريدكتاز لكن
+
+79
+00:05:37,730 --> 00:05:41,290
+هان بيصير في عندي complete reduction هان لأ فقط
+
+80
+00:05:41,290 --> 00:05:46,670
+للنيترات بعدين ممكن انه يتم الاستفادة منه اختزاله
+
+81
+00:05:46,670 --> 00:05:50,950
+لامونيوم حتى احصل على ال nitrogen source بالنسبة
+
+82
+00:05:50,950 --> 00:05:56,190
+لل procedure كيف بدي اكشف عن هذا الانزيمأكيد هجيب
+
+83
+00:05:56,190 --> 00:05:59,770
+ميديا يكون فيها ال substrate اللى هيشتغل فيه عليها
+
+84
+00:05:59,770 --> 00:06:03,850
+الانزيم ال media اسمها نيترات ال broth بتكون ال
+
+85
+00:06:03,850 --> 00:06:08,410
+broth فبالتالي طريقة الزراعة باخد انيكولام باخد
+
+86
+00:06:08,410 --> 00:06:11,090
+البكتيريا اللى بدي أكشف هل عندها الانزيم ولا لأ
+
+87
+00:06:11,090 --> 00:06:15,270
+بعمل mix فيه ال broth هاي ال نيترات ال broth بقولى
+
+88
+00:06:15,270 --> 00:06:20,810
+بتحتوي على نصف المية KNO3 يبقى ال substrate اللى
+
+89
+00:06:20,810 --> 00:06:24,710
+هيشتغل عليها الانزيم ال NO3بتكون موجودة في هذه
+
+90
+00:06:24,710 --> 00:06:29,130
+الميديا بعد ما أزرع البكتيري هعمل incubation لمدة
+
+91
+00:06:29,130 --> 00:06:35,910
+24 ساعة على 37 درجة سليسيا في ال incubatorهلأ لو
+
+92
+00:06:35,910 --> 00:06:41,470
+البكتيريا عندها أنزيم النيترات Reductase ممكن انه
+
+93
+00:06:41,470 --> 00:06:45,470
+يكون ناتج التفاعل نيترات ممكن يكون أمانيا ممكن
+
+94
+00:06:45,470 --> 00:06:51,250
+يكون molecular نيتروجين كيف بدي أكشف عن هدولة بقول
+
+95
+00:06:51,250 --> 00:06:56,820
+بتضيف بعد الincubation reagentزي ما قلنا اي تفاعل
+
+96
+00:06:56,820 --> 00:07:00,840
+كيف بدى اكشف عنه اما بشوفه باعينى او ممكن اضيف بيه
+
+97
+00:07:00,840 --> 00:07:04,760
+H Indicator اذا كان ناتج التفاعل acid او base
+
+98
+00:07:04,760 --> 00:07:10,120
+فبالتالي هيغير لون الوسط او ممكن اضيف reagent هذا
+
+99
+00:07:10,120 --> 00:07:16,530
+ال reagent بيتفاعل مع احد نواتج التفاعل تمامفهنا
+
+100
+00:07:16,530 --> 00:07:20,330
+طبعا ماعندي ازد ولا بيز مش هقدر ايه اشوف ناتج
+
+101
+00:07:20,330 --> 00:07:23,990
+التفاعل بقى يعني ماعندي جاز مثلا فبالتالي هضيف
+
+102
+00:07:23,990 --> 00:07:27,850
+reactant هذا ال reactant هيتفاعل مع احد نواتج
+
+103
+00:07:27,850 --> 00:07:33,570
+التفاعل بقوللي بضيف بعد ال incubation زي فحص ال
+
+104
+00:07:33,570 --> 00:07:37,010
+methyl red vocosper scour كنا نضيف ال reactant بعد
+
+105
+00:07:37,010 --> 00:07:40,210
+ال incubation نعمل زراعة و نضيف بعد ال incubation
+
+106
+00:07:40,210 --> 00:07:44,670
+هذا نفس الفكرة نضيف بعد ال incubationReagent A
+
+107
+00:07:44,670 --> 00:07:49,750
+اسمه Sulphanilic Acid Reagent B بضيفه Diamethyl
+
+108
+00:07:49,750 --> 00:07:54,010
+Nafthalamine بضيف خمسة drop من Reagent A وخمسة
+
+109
+00:07:54,010 --> 00:08:00,830
+drop من Reagent B وبشوف التفاعل بقوللي ان Reagent
+
+110
+00:08:00,830 --> 00:08:06,970
+A و Reagent B بيتفاعلوا مع ال Nitrite بيتفاعلوا مع
+
+111
+00:08:06,970 --> 00:08:11,490
+ال Nitrite قولنا أي Reagent بضيفه بيتفاعل مع نواتج
+
+112
+00:08:11,490 --> 00:08:15,420
+التفاعلreagent A و B بتفاعل مع مين بالظبط من نواتج
+
+113
+00:08:15,420 --> 00:08:21,820
+التفاعل بتفاعل مع ال nitrite بقول لي لو ال nitrite
+
+114
+00:08:21,820 --> 00:08:24,560
+لو تم اغتزال ال nitrite ل Nitrite و ال nitrite
+
+115
+00:08:24,560 --> 00:08:29,280
+موجود هيرتبط ب reagent A و B و هيعطيني red complex
+
+116
+00:08:29,280 --> 00:08:35,660
+فبالتالي لو اتحول لون ال meat يبعد إضافة reagent A
+
+117
+00:08:35,660 --> 00:08:39,880
+و B للون ال red معناه هاي ان البكتيريا عندها أنزيم
+
+118
+00:08:39,880 --> 00:08:45,010
+ال nitrate reductaseحولت ال nitrate ل nitrite يبقى
+
+119
+00:08:45,010 --> 00:08:49,070
+high positive من الأمثلة عليها ال E coli انها حولت
+
+120
+00:08:49,070 --> 00:08:52,290
+ال nitrate ل nitrite ال nitrite اللى فى ال media
+
+121
+00:08:52,290 --> 00:08:56,030
+اتفاعل مع ال reagent a و b اللى انا ضفتهم اعطانى
+
+122
+00:08:56,030 --> 00:09:01,490
+red complex طيب لما اتغير اللون بقولى ما بقدر اكتب
+
+123
+00:09:01,490 --> 00:09:06,110
+انه negative ما بقدر احكى ان هذه النتيجة negative
+
+124
+00:09:06,110 --> 00:09:11,180
+ليش لأن احنا قلناإن ناتج التفاعل أو البكتيريا اللي
+
+125
+00:09:11,180 --> 00:09:13,720
+عندها أنزيم الـNitrate Reductase ممكن يكون ناتج
+
+126
+00:09:13,720 --> 00:09:19,020
+التفاعل Nitrite ممكن بعد ما يتم يكون الـNitrite في
+
+127
+00:09:19,020 --> 00:09:23,040
+الميديا يتم اختزاله لأمونيوم ممكن برضه إنه يصير في
+
+128
+00:09:23,040 --> 00:09:26,880
+عندي Complete Reduction ويتم تحويل الـNitrate عبر
+
+129
+00:09:26,880 --> 00:09:30,480
+سلسلة من الإنزيمات وسلسلة من التفاعلات لموليكولر
+
+130
+00:09:30,480 --> 00:09:36,540
+نيتروجينفبالتالي لو طلع النتيجة colorless هذا
+
+131
+00:09:36,540 --> 00:09:40,300
+لايعني انه negative ممكن يكون فعلا البكتيريا ما
+
+132
+00:09:40,300 --> 00:09:44,480
+عندها الانزيموممكن ان البكتيريا يكون عندها الانزيم
+
+133
+00:09:44,480 --> 00:09:49,360
+لكن حولت النيتريات لأمونيوم او حولت النيتريات
+
+134
+00:09:49,360 --> 00:09:53,080
+لموليكيولا نيتروجين في صار عندها complete
+
+135
+00:09:53,080 --> 00:09:58,180
+reduction طيب كيف بدي اعرف هل صار في عندي complete
+
+136
+00:09:58,180 --> 00:10:02,960
+reduction او اتحول لأمونيوم او فعلا البكتيريا ما
+
+137
+00:10:02,960 --> 00:10:06,860
+عندها انزيم النيتريات الريدكتاز بقوللي بضيفي
+
+138
+00:10:06,860 --> 00:10:12,280
+reaction C في حالكان اللون colorless ما اتغير بعد
+
+139
+00:10:12,280 --> 00:10:16,880
+إضافة reagent a و b بتضيفي reagent c اللي هو ال
+
+140
+00:10:16,880 --> 00:10:21,760
+zinc powder ال zinc powder يعتبر reducing agent
+
+141
+00:10:21,760 --> 00:10:27,620
+طبعا هضيف reagent c بقوللي بعد إضافة reagent c في
+
+142
+00:10:27,620 --> 00:10:32,520
+حال كانت النتيجة colorless بعد إضافة reagent a و b
+
+143
+00:10:32,520 --> 00:10:37,680
+إذا اللون اتحول للون red معناه هي النتيجة negative
+
+144
+00:10:38,400 --> 00:10:43,660
+لكن لو ما اتغير معناه هذه النتيجة انها positive
+
+145
+00:10:43,660 --> 00:10:47,220
+صار عندي complete reduction اتحول ال nitrate ل
+
+146
+00:10:47,220 --> 00:10:50,620
+molecular نيتروجين او ال nitrate بعد ما اتحول ل
+
+147
+00:10:50,620 --> 00:10:54,420
+nitrite اتحول ل ammonia طيب ليش احنا قولنا ان
+
+148
+00:10:54,420 --> 00:10:58,080
+النتيجة ال red negative و هنقولنا ان النتيجة ال
+
+149
+00:10:58,080 --> 00:11:02,800
+red positive الفكرة ان ال zinc powder يعتبر
+
+150
+00:11:02,800 --> 00:11:08,300
+reducing agent ايش يعني reducing agentيعني هيشتغل
+
+151
+00:11:08,300 --> 00:11:12,340
+شغل البكتيريا اللى عندها انزيم الـ Nitrate
+
+152
+00:11:12,340 --> 00:11:17,040
+Reductase لو البكتيريا ما عندها الانزيم هان الـ
+
+153
+00:11:17,040 --> 00:11:19,620
+negative لو ما عندها انزيم الـ Nitrate Reductase
+
+154
+00:11:19,620 --> 00:11:24,380
+هذا يعني انه ماصار تفاعل النيترات دلوا موجود في
+
+155
+00:11:24,380 --> 00:11:28,740
+الميديا السبسترات دلت موجودة في الميديا ماصار في
+
+156
+00:11:28,740 --> 00:11:33,020
+عندي اي تفاعل النيترات ما اتفاعل دلت موجودة في
+
+157
+00:11:33,020 --> 00:11:37,640
+الميدياهيجيز zinc powder اللى يعتبر reducing agent
+
+158
+00:11:37,640 --> 00:11:42,540
+هيشتغل شغل الانزيم هيحول ال nitrate اللى موجودة في
+
+159
+00:11:42,540 --> 00:11:48,460
+ال media لمعصر فيها اى تفاعل ل nitrite ال nitrite
+
+160
+00:11:48,460 --> 00:11:51,360
+تمام هيك صار عندى nitrite موجودة في ال media ال
+
+161
+00:11:51,360 --> 00:11:55,380
+nitrite انا ضفت قبلها reagent a و b سابقا كان مضعف
+
+162
+00:11:55,380 --> 00:12:00,530
+reagent a و b لما صار colorlessهيتفاعلوا reaction
+
+163
+00:12:00,530 --> 00:12:06,590
+a و b مع الـ nitrite اللى تم اختزاله بواسطة ال
+
+164
+00:12:06,590 --> 00:12:11,810
+zinc powder و ليس بواسطة البكتيريا لأنه ليش تم
+
+165
+00:12:11,810 --> 00:12:18,050
+تحويله لأن ال nitrate لم يتم استهلاكها كانت
+
+166
+00:12:18,050 --> 00:12:20,850
+البكتيريا معاها أنزيم ال nitrate reductase فضلت
+
+167
+00:12:20,850 --> 00:12:24,010
+موجودة في ال media فبالتالي هيش ال zinc powder
+
+168
+00:12:24,010 --> 00:12:28,380
+هيعملها reduction لل nitriteهيتفاعل مع reactant A
+
+169
+00:12:28,380 --> 00:12:32,340
+و B الموجود سابقاً فهيتحول اللون للون Red فبالتالي
+
+170
+00:12:32,340 --> 00:12:36,640
+اللون ال Red بعد إضافة reactant C اللي هو ال zinc
+
+171
+00:12:36,640 --> 00:12:40,880
+powder يعتبر negative من الأمثلة على البكتيريا I
+
+172
+00:12:40,880 --> 00:12:44,080
+اللي ما عندها الانزيم النيترات ريداكتاز اللي هو
+
+173
+00:12:44,080 --> 00:12:51,480
+لستاف Epidermidis طيب بالنسبة للبعد إضافة Reagent
+
+174
+00:12:51,480 --> 00:12:55,060
+C إذا دلت colorless قولنا هذا يعني انه بتعمل
+
+175
+00:12:55,060 --> 00:12:58,720
+complete reduction او حولت ال nitrate ل ammonia
+
+176
+00:12:58,720 --> 00:13:04,490
+لسه عندي colorlessلأن reagent C هو يعتبر reducing
+
+177
+00:13:04,490 --> 00:13:09,570
+agent هيشتغل على النيترات طيب في حال كان عندي
+
+178
+00:13:09,570 --> 00:13:13,470
+complete reduction النيترات موجود في الميديا لأ تم
+
+179
+00:13:13,470 --> 00:13:17,630
+استهلاكه تم تحويله لموليكيولر نيتروجين أو تم
+
+180
+00:13:17,630 --> 00:13:21,830
+تحويله لأمونيام فبالتالي النيترات اللي هيشتغل
+
+181
+00:13:21,830 --> 00:13:25,870
+عليها ال zinc powder مش موجود فبالتالي مش هيصير اي
+
+182
+00:13:25,870 --> 00:13:29,710
+تفاعل مش هيتحول النيترات لنيترات مش هيرتبط ب
+
+183
+00:13:29,710 --> 00:13:34,210
+reagent اوأو بي مش هيتحول اللون للون أحمر فبالتالي
+
+184
+00:13:34,210 --> 00:13:38,830
+ال colorless بعد إضافة reaction C يعتبر معناه إنه
+
+185
+00:13:38,830 --> 00:13:41,890
+البكتيريا عملت complete reduction حولت ال nitrate
+
+186
+00:13:41,890 --> 00:13:46,230
+ل molecular nitrogen أو أمونيا من الأمثلة على
+
+187
+00:13:46,230 --> 00:13:49,270
+البكتيريا اللي بتعمل complete reduction الصدوموناس
+
+188
+00:13:49,270 --> 00:13:55,300
+يبقى الصدوموناس لو أنا ضفت reaction A أو Bهتعطيني
+
+189
+00:13:55,300 --> 00:13:59,940
+colorless لكن بعد إضافة reagent C هتظلها colorless
+
+190
+00:13:59,940 --> 00:14:04,580
+ال E.coli فقط هتعمل reduction للنيترات مش هتعمل
+
+191
+00:14:04,580 --> 00:14:07,500
+complete reduction عشان هيك لما أضيف reagent A و B
+
+192
+00:14:07,500 --> 00:14:10,540
+بسرعة هلاحظ أنه في عندي لون أحمر هذا معناه أن
+
+193
+00:14:10,540 --> 00:14:14,280
+البكتيريا عندها أنزيم النيتريات Reductase لكن
+
+194
+00:14:14,280 --> 00:14:17,200
+ماعملت complete reduction فقط حولت النيتريات
+
+195
+00:14:17,200 --> 00:14:21,140
+لنيترات من الأمثلة عليها ال E.coli بعد إضافة
+
+196
+00:14:21,140 --> 00:14:25,680
+reagent C إذا كان اللون Redهذا معناه ان ما عندها
+
+197
+00:14:25,680 --> 00:14:28,820
+الانزيم من الأمثلة عليها الـ Staph Epidermis ليش
+
+198
+00:14:28,820 --> 00:14:32,880
+اتحول اللون للون Red لأن Zinc Powder اشتغل شغل
+
+199
+00:14:32,880 --> 00:14:37,680
+الانزيم اشتغل على ال Nitrate اللي لم يتم استهلاكها
+
+200
+00:14:37,680 --> 00:14:40,660
+لأنها ما عندها الانزيم فضلت موجودة في ال media
+
+201
+00:14:40,660 --> 00:14:44,380
+اشتغل عليها ال Zinc Powder يبقى اللون ال Red كان
+
+202
+00:14:44,380 --> 00:14:48,260
+ناتج من ال Zinc Powder اللي يعتبر reducing agent
+
+203
+00:14:48,260 --> 00:14:54,460
+وليس من البكتيريافاشتغل عليها و اعطاني ال nitrite
+
+204
+00:14:54,460 --> 00:14:57,520
+ال nitrite اتفاعل مع ال reagent a و b اللى موجود
+
+205
+00:14:57,520 --> 00:15:00,640
+سابقا و اعطاني اللون ال red لكن ال zinc powder
+
+206
+00:15:00,640 --> 00:15:04,520
+مالاقى اشي في حال ال complete reduction مالاقى ال
+
+207
+00:15:04,520 --> 00:15:07,520
+nitrate عشان يشتغل عليها فبالتالي مش هيصير اي
+
+208
+00:15:07,520 --> 00:15:10,600
+تفاعل لأنه صار عندي complete reduction اتحول ال
+
+209
+00:15:10,600 --> 00:15:14,860
+nitrate ل molecular nitrogen فمش موجود ال nitrate
+
+210
+00:15:14,860 --> 00:15:20,410
+تم استهلاكه في التفاعلهذا بالنسبة للـ procedure و
+
+211
+00:15:20,410 --> 00:15:23,890
+أمثلة على البكتيريا اللى بتتحول فقط الـ nitrate ل
+
+212
+00:15:23,890 --> 00:15:28,710
+nitrite أمثلة على البكتيريا اللى بتتحول ال nitrate
+
+213
+00:15:28,710 --> 00:15:31,250
+ل molecular nitrogen بتعمل complete reduction
+
+214
+00:15:31,250 --> 00:15:34,410
+الصدمه و نصب البكتيريا اللى ما عندها أنزيم للـ
+
+215
+00:15:34,410 --> 00:15:38,490
+Staphylococcus epidermidisهذا ملخص فحص الـ Nitrate
+
+216
+00:15:38,490 --> 00:15:42,550
+Reduction Test الـ Principle أشهر الـ procedure من
+
+217
+00:15:42,550 --> 00:15:46,090
+الـ positive و الـ negative للفحص يبقى لو جيبنا
+
+218
+00:15:46,090 --> 00:15:52,350
+سؤال صح و غلط أنه بعد إضافة Reagent C اللون إذا
+
+219
+00:15:52,350 --> 00:15:57,130
+اتحول للون أحمر هذا معناه أن النتيجة positive غلط
+
+220
+00:15:57,500 --> 00:16:00,960
+بعد إضافة Reagency إذا اتحول اللون للون أحمر هذا
+
+221
+00:16:00,960 --> 00:16:05,640
+معناه إنه نتيجة negative اللون الأحمر نتج نتيجة
+
+222
+00:16:05,640 --> 00:16:10,460
+إنه تم اختزال ال Nitrate ل Nitrite باستخدام ال
+
+223
+00:16:10,460 --> 00:16:15,360
+Zinc Powder وليس بسبب البكتيريا أو الإنزيم الموجود
+
+224
+00:16:15,360 --> 00:16:21,390
+في البكتيريانمر على ال slide ع السريع organism
+
+225
+00:16:21,390 --> 00:16:24,850
+that poses the enzyme nitrate reductase البكتيريا
+
+226
+00:16:24,850 --> 00:16:28,290
+اللي عندها أنزيم النيترات ريدكتاز هتعمل اختزال من
+
+227
+00:16:28,290 --> 00:16:32,550
+النيترات لنيترات قلنا النيترات هو ال substrate
+
+228
+00:16:32,550 --> 00:16:36,310
+لأنزيم النيترات الريدكتاز بقول النيترات إذا
+
+229
+00:16:36,310 --> 00:16:39,830
+ملاحظين هي النيترات كيف بدي أكشف عنه قلنا انه
+
+230
+00:16:39,830 --> 00:16:44,200
+reagent a و bهيتفاعلوا فقط مع النيترات مع ناتج
+
+231
+00:16:44,200 --> 00:16:47,100
+التفاعل النيترات وليس مع ال ammonium وليس مع ال
+
+232
+00:16:47,100 --> 00:16:51,780
+molecular nitrogen فبالتالي لو كانت النتيجة ما
+
+233
+00:16:51,780 --> 00:16:55,640
+تغير اللون هذا لا يعني انه ما عندها الانزيم ممكن
+
+234
+00:16:55,640 --> 00:16:59,290
+انها تكون عاملة complete reductionمش موجود اندي
+
+235
+00:16:59,290 --> 00:17:03,630
+ناتج التفاعل نيتريت موجود موليكولر نيتروجين او
+
+236
+00:17:03,630 --> 00:17:08,250
+امونيام فبالتالي ماتفعلوا مع reagent A و B فهضيف
+
+237
+00:17:08,250 --> 00:17:11,590
+اللي هو ال zinc powder كيف بدي اكشف على ال nitrite
+
+238
+00:17:11,590 --> 00:17:16,250
+ion بقول بإضافة sulfanilic acid reagent A ما
+
+239
+00:17:16,250 --> 00:17:20,620
+ذكرناه اللي هو يعتبراسمه salphanilic acid reagent
+
+240
+00:17:20,620 --> 00:17:24,980
+باسمه الـ dimethyl naphthalamide يبقىها reagent A
+
+241
+00:17:24,980 --> 00:17:28,360
+salphanilic acid reagent B dimethyl naphthalamide
+
+242
+00:17:28,360 --> 00:17:32,800
+هضيفهم على المزرعة بعد ال incubation 24 ساعة
+
+243
+00:17:32,800 --> 00:17:37,340
+بقوللي any nitrite in the media أي نيتريت موجود في
+
+244
+00:17:37,340 --> 00:17:40,520
+ال media هيتفاعل مع هذه ال reagent وهيعطيني red
+
+245
+00:17:40,520 --> 00:17:44,520
+color يبقىبعد إضافة reagent A و B إذا اتحول اللون
+
+246
+00:17:44,520 --> 00:17:49,620
+للون Red معناه هاي أنه تم تحويل ال Nitrate ل
+
+247
+00:17:49,620 --> 00:17:54,360
+Nitrite باستخدام إنزيم ال Nitrate Reductase هذه
+
+248
+00:17:54,360 --> 00:17:57,860
+الصورة للتفاعل قلنا ال Nitrate هي ال substrate هي
+
+249
+00:17:57,860 --> 00:18:01,260
+تم اختزالها ل Nitrite ممكن ال Nitrate بعض أنواع
+
+250
+00:18:01,260 --> 00:18:05,140
+البكتيريا تعملوا اختزال ل Ammonium وممكن يصير في
+
+251
+00:18:05,140 --> 00:18:08,410
+عندي complete reductionأخر احصل على molecular
+
+252
+00:18:08,410 --> 00:18:11,970
+nitrogen يعني في النهاية هحصل على nitrogen source
+
+253
+00:18:11,970 --> 00:18:16,830
+لازم اللي هو البكتيريا بقولي لو البكتيريا ما تغير
+
+254
+00:18:16,830 --> 00:18:21,970
+لونها مصر فين دي اي تغير في اللون في بعد إضافة
+
+255
+00:18:21,970 --> 00:18:26,230
+reagent a و b هذا معناه ان البكتيرياThe bacteria
+
+256
+00:18:26,230 --> 00:18:29,350
+bosses nitrate reductase عندها البكتيريا نيترات
+
+257
+00:18:29,350 --> 00:18:34,630
+ردكتاز لكن عملت reduce لالنيترات لأمونيوم قلنا
+
+258
+00:18:34,630 --> 00:18:37,810
+ممكن بعد ما يتم اختزال النيترات لنيترات يتم
+
+259
+00:18:37,810 --> 00:18:42,070
+اختزاله لأمونيوم أو ممكن انه يتم اختزاله ل
+
+260
+00:18:42,070 --> 00:18:46,480
+molecular نيتروجين في سلسلة من التفاعلاتهذه نفس
+
+261
+00:18:46,480 --> 00:18:49,760
+الفكرة انه عندها الانزيم لكن عملت اختيار للنيترات
+
+262
+00:18:49,760 --> 00:18:54,620
+لامونيا او الاحتمال التالت انه البكتيريا ما عندها
+
+263
+00:18:54,620 --> 00:18:58,480
+انزيم النيترات Reductase فبالتالي ما صار reduction
+
+264
+00:18:58,480 --> 00:19:05,350
+للنيترات بقول لي عشانأحدد هل البكتيريا عندها
+
+265
+00:19:05,350 --> 00:19:09,850
+الانزيم عملت complete reduction أو حولته لأمونيوم
+
+266
+00:19:09,850 --> 00:19:13,810
+أو فعلا هي ما عندها الانزيم هضيف ال zinc powder a
+
+267
+00:19:13,810 --> 00:19:16,190
+small amount of zinc powder is added to the
+
+268
+00:19:16,190 --> 00:19:20,050
+culture containing the reagent هيه هضيف ال zinc
+
+269
+00:19:20,050 --> 00:19:24,810
+powder للمزرعة اللي بتحتوي على reagent A وB سابقا
+
+270
+00:19:25,450 --> 00:19:29,050
+بقولي الـ Zinc إيش وظيفته؟ الـ Zinc Powder يُعتبر
+
+271
+00:19:29,050 --> 00:19:32,710
+قولنا الـ Reducing Agent Reduce نيتريات و نيتريات
+
+272
+00:19:32,710 --> 00:19:36,730
+قولنا هيعمل اختزال للنيتريات اللي موجود في الميديا
+
+273
+00:19:36,730 --> 00:19:42,250
+لنيتريات فبالتالي a pink أو red color أبير هيتحول
+
+274
+00:19:42,250 --> 00:19:46,350
+لون الوسط للون أحمر لأنه تم اختزال النيتريات
+
+275
+00:19:46,350 --> 00:19:49,890
+لنيتريات بواسطة الـ Zinc Powder وليس بواسطة
+
+276
+00:19:49,890 --> 00:19:53,510
+البكتيريا الـ Nitrite موجود في الميديا قولنا الـ
+
+277
+00:19:53,510 --> 00:19:57,140
+Nitriteبرتبط بـ reagent A و B الموجودين في الميديا
+
+278
+00:19:57,140 --> 00:20:01,160
+فيعطيين اللون الأحمر بقولي الحقيقة أن الـ Nitrate
+
+279
+00:20:01,160 --> 00:20:05,280
+were not reduced to nitrite by the bacteria في هذه
+
+280
+00:20:05,280 --> 00:20:08,860
+الحالة أن الـNitrate لم يتم اختزاله لـNitrite
+
+281
+00:20:08,860 --> 00:20:12,300
+باستخدام البكتيريا تم اختزاله باستخدام الـ Zinc
+
+282
+00:20:12,300 --> 00:20:16,800
+powder طيب ليش البكتيريا ليش ال Zinc powder قدر
+
+283
+00:20:16,800 --> 00:20:19,460
+انه يختزل من ال Nitrate لـNitrite؟ بقولي لأن
+
+284
+00:20:19,460 --> 00:20:24,830
+الـNitrate لم يتم استهلاكه Nitrate unreactedلم يتم
+
+285
+00:20:24,830 --> 00:20:30,050
+استهلاكه فهذا يعني ان البكتيريا ما عندها انزيم ال
+
+286
+00:20:30,050 --> 00:20:34,910
+nitrate reductase لكن ال red color doesn't appear
+
+287
+00:20:34,910 --> 00:20:38,730
+اذا ما ظهر اللون الأحمر هذا يعني ان ال nitrate
+
+288
+00:20:38,730 --> 00:20:42,810
+الموجود في ال media تم اختزاله سابقا ل nitrite
+
+289
+00:20:43,250 --> 00:20:46,750
+بعدها تم اختزاله ل ammonium or nitrogen gas
+
+290
+00:20:46,750 --> 00:20:50,690
+فبالتالي هان انه ال nitrate ال substrate تم
+
+291
+00:20:50,690 --> 00:20:54,810
+استهلاكها فلما ييجي ال zinc powder هيشتغل عليها مش
+
+292
+00:20:54,810 --> 00:20:57,690
+هلاقى مش هيلاقي اشي يشتغل عليها فبالتالي مش هيصير
+
+293
+00:20:57,690 --> 00:21:01,330
+اي تفاعل مش هيصير اي تغير في اللون فهذا يعني انه
+
+294
+00:21:01,330 --> 00:21:05,090
+صار في عندي complete reduction او اتحول ال nitrate
+
+295
+00:21:05,090 --> 00:21:10,810
+تم اختزاله ل ammoniumكيف ال procedure قلنا بستخدم
+
+296
+00:21:10,810 --> 00:21:14,470
+نيترات ال breath فيها بوتاسيوم نيترات نصف المية
+
+297
+00:21:14,470 --> 00:21:19,010
+بعمل incubation هضيف خمسة drop من reactant A وخمسة
+
+298
+00:21:19,010 --> 00:21:24,070
+drop من reactant B بقولي if nitrate is present in
+
+299
+00:21:24,070 --> 00:21:27,850
+the media it will turn red within one to two
+
+300
+00:21:27,850 --> 00:21:33,090
+minutes يعني ان في غلط في ال slide نغيرها ل
+
+301
+00:21:33,090 --> 00:21:37,420
+nitriteلو الـ Nitrite موجودة في الميديا قلنا
+
+302
+00:21:37,420 --> 00:21:40,760
+هتتفاعل مع reagent A و B و هتعطيني اللون الأحمر
+
+303
+00:21:40,760 --> 00:21:45,000
+يبقى ال positive هي red color بعد إضافة reagent A
+
+304
+00:21:45,000 --> 00:21:52,060
+و B لو ما تغير قلنا مابنحكي negative مابنحكي
+
+305
+00:21:52,060 --> 00:21:56,040
+negative ال negative test بعد إضافة reagent A و B
+
+306
+00:21:56,040 --> 00:22:00,840
+قلنا مانضيف zinc powder لو اتحول اللون للون الأحمر
+
+307
+00:22:00,840 --> 00:22:05,380
+هذا معناه إنه نتيجة negativeلأن الذنك بودر اشتغل
+
+308
+00:22:05,380 --> 00:22:08,900
+على الـ Nitrate اللي لم يتم استهلاكه لأن البكتيريا
+
+309
+00:22:08,900 --> 00:22:14,040
+ما عندها انزيم الـ Nitrate Reductase لكن النتيجة
+
+310
+00:22:14,040 --> 00:22:16,940
+الـ Positive معناه انه عملت البكتيريا Complete
+
+311
+00:22:16,940 --> 00:22:20,860
+Reduction او تم اختزال الـ Nitrate لأمونيوم
+
+312
+00:22:20,860 --> 00:22:24,260
+فبالتالي اجى الذنك بودر هيشتغل على الـ Nitrate ما
+
+313
+00:22:24,260 --> 00:22:28,520
+لقى الـNitrate تم استهلاكها فبالتالي ماهيصير تفاعل
+
+314
+00:22:28,520 --> 00:22:33,450
+هيكون Colorless يبقى هنعدل انهمش الـ Nitrate لو
+
+315
+00:22:33,450 --> 00:22:36,590
+الـ Nitrate موجود في الـ media هيتحول اللون للون
+
+316
+00:22:36,590 --> 00:22:43,730
+أحمر لأن هو اللي هيرتبط بـ reagent A و B هان
+
+317
+00:22:43,730 --> 00:22:46,550
+التفاعل احنا قلنا في هندي أكتر من احتمال ممكن
+
+318
+00:22:46,550 --> 00:22:50,430
+البكتيريا يتحول ال Nitrate ل Nitrite ممكن يتم
+
+319
+00:22:50,430 --> 00:22:54,630
+تحويل ال Nitrate ل molecular nitrogen لإنه تعمل
+
+320
+00:22:54,630 --> 00:22:58,450
+complete reduction قلنا ممكن بعد ما يتم اختزال ال
+
+321
+00:22:58,450 --> 00:23:03,080
+Nitrate ل Nitrite انهايختزل الـ Nitrate لـ Ammonia
+
+322
+00:23:03,080 --> 00:23:06,800
+يعني يكون التفاعل اختزال الـ Nitrate لـ Ammonia
+
+323
+00:23:06,800 --> 00:23:09,220
+ممكن مايصير في نتيجة الـ Negative هذه التفاعل هذه
+
+324
+00:23:09,220 --> 00:23:15,100
+النتيجة الـ Positive هذا معناه أنه عندها الانزيم
+
+325
+00:23:15,100 --> 00:23:21,000
+لكن نتائج التفاعل بتختلف هنا نتيجة التفاعل Nitrate
+
+326
+00:23:21,000 --> 00:23:26,840
+هنا نيتروجين جاز هنا Ammonia الرابع واحدة هي ما
+
+327
+00:23:26,840 --> 00:23:31,250
+عندها انزيم يبقى نقطةهذه هي الـ Positive
+
+328
+00:24:21,150 --> 00:24:23,390
+لكن هاي اخر issue هي negative
+
+329
+00:24:47,360 --> 00:24:51,440
+يبقى سواء كان ناتج التفاعل نيتريت أو molecular
+
+330
+00:24:51,440 --> 00:24:54,680
+nitrogen أو أمونيوم هذا معناه أن البكتيريا عندها
+
+331
+00:24:54,680 --> 00:24:59,300
+الانزيم لكن هانصار في ياندي complete reduction لو
+
+332
+00:24:59,300 --> 00:25:03,660
+ماصار تفاعل يبقى أو ماعندها البكتيريا الانزيم يبقى
+
+333
+00:25:03,660 --> 00:25:07,140
+ماهيصير تفاعل فبالتالي النيتريات ال substrate هتظل
+
+334
+00:25:07,140 --> 00:25:11,340
+موجودة في ال media أكيد بالنسبة لل nitrogen gas
+
+335
+00:25:11,340 --> 00:25:15,320
+مافيش كلهم موجود لكن في حال كانت عملة البكتيريا
+
+336
+00:25:15,320 --> 00:25:18,460
+complete reduction حولتالنيترات لـ molecular
+
+337
+00:25:18,460 --> 00:25:23,960
+nitrogen في عندي بيكون nitrogen gas بعد ما اضيف
+
+338
+00:25:23,960 --> 00:25:28,580
+reagent A و B يقولنا اذا اتحول اللون للون red هذا
+
+339
+00:25:28,580 --> 00:25:34,300
+معناه انه البكتيريا حولت النيترات لنيترات فقطليش؟
+
+340
+00:25:34,300 --> 00:25:39,420
+لأن قلنا إن الـreagent A وB بيتفاعل مع الـnitrite
+
+341
+00:25:39,420 --> 00:25:43,400
+ما بيتفاعل مع الـnitrogen molecular nitrogen أو
+
+342
+00:25:43,400 --> 00:25:46,620
+الـammonium أو الـnitrate في حال كانت البكتيريا
+
+343
+00:25:46,620 --> 00:25:49,840
+negative يبقى بعد إضافة reagent A وB إذا اتحول
+
+344
+00:25:49,840 --> 00:25:54,680
+اللون للون Red منشوف التفاعل إذا ناتج التفاعل
+
+345
+00:25:54,680 --> 00:25:58,760
+Nitrite يبقى نحكي إنه في عندي هيتحول اللون للون
+
+346
+00:25:58,760 --> 00:26:01,940
+Red لكن لو كان ناتج التفاعل مش Nitrite يبقى كلهم
+
+347
+00:26:01,940 --> 00:26:05,350
+هانمش ناتج التفاعل نيتريت مش ناتج التفاعل نيتريت
+
+348
+00:26:05,350 --> 00:26:11,530
+مش عندى تفاعل المواد المتفاعلة نيتريت مش نيتريت
+
+349
+00:26:11,530 --> 00:26:14,750
+يبقى مش هيصير ثغير فى اللون لأن ال reagent A و B
+
+350
+00:26:14,750 --> 00:26:18,510
+بيرتبط مع ال نيتريت فقط فى حال كان ناتج التفاعل
+
+351
+00:26:18,510 --> 00:26:24,090
+نيتريت هو هحكي هان انه هيتحول اللون للون Red طيب
+
+352
+00:26:24,090 --> 00:26:28,250
+بقولى بعد مضيف ال zinc powder أكيد فى حال كان
+
+353
+00:26:28,250 --> 00:26:32,500
+البكتيريا حولت ال نيتريت لنيتريتما بضيف الذنك
+
+354
+00:26:32,500 --> 00:26:36,420
+باودر فقط بضيف الذنك باودر في حال ما تغير اللون
+
+355
+00:26:36,420 --> 00:26:40,080
+بعد إضافة reagent A وB في هذه الحالة ما بضيف الذنك
+
+356
+00:26:40,080 --> 00:26:43,860
+باودر بعتبرها positive على طول لكن في حال ما تغير
+
+357
+00:26:43,860 --> 00:26:47,340
+اللون بضيف الذنك باودر عشان أفرق من البكتيريا اللي
+
+358
+00:26:47,340 --> 00:26:50,020
+ما عندها ال enzyme ومن البكتيريا اللي عملت
+
+359
+00:26:50,020 --> 00:26:54,110
+complete reductionبعد إضافة الزنك باودر إذا اتحول
+
+360
+00:26:54,110 --> 00:26:58,190
+اللون للون pink to red هاي معناه إنه البكتيريا ما
+
+361
+00:26:58,190 --> 00:27:02,430
+عندها الانزيم لأنه قلنا زنك باودر بيشتغل على قياشة
+
+362
+00:27:02,430 --> 00:27:07,430
+بيشتغل على النيترات فوان هشوف نيترات يبقى هيتحول
+
+363
+00:27:07,430 --> 00:27:11,090
+اللون للون red لكن هان في حال كانت عملت complete
+
+364
+00:27:11,090 --> 00:27:14,350
+reduction أو في حال اتحول النيترات لأمونيوم أكيد
+
+365
+00:27:14,350 --> 00:27:20,050
+بعد إضافة زنك باودر مش هيتغير اللون عنديلأنه مش
+
+366
+00:27:20,050 --> 00:27:23,730
+هيشتغل مش موجود ال substrate اللي هي ال nitrate
+
+367
+00:27:23,730 --> 00:27:26,970
+اللي هيشتغل عليها فبالتالي مش هيصير تفاعل لكن في
+
+368
+00:27:26,970 --> 00:27:30,970
+حالة ال negative أكيد ال nitrate لم يتم استهلاك
+
+369
+00:27:30,970 --> 00:27:34,530
+هضلت في الموجودة في ال media هيجي ال zinc powder
+
+370
+00:27:34,530 --> 00:27:38,010
+اللي هيشتغل نفس شغل الإنزيم هيحولها ل Nitrite ال
+
+371
+00:27:38,010 --> 00:27:41,930
+nitrite هترتبط مع ال agent A و B اللي مضاعف سابقا
+
+372
+00:27:41,930 --> 00:27:43,630
+فهيتحول اللون للون Red
+
+373
+00:27:50,080 --> 00:27:55,620
+هي الصورة في الميديا فيها نيتريات ضفت reagent a وb
+
+374
+00:27:55,620 --> 00:28:00,540
+ماتغير اللون فساعت وقتها هضيف ال zinc powder بعد
+
+375
+00:28:00,540 --> 00:28:04,180
+إضافة ال zinc powder إذا اتحول اللون للون red هذا
+
+376
+00:28:04,180 --> 00:28:12,060
+معناه إنه النتيجة negative ضفت reagent هاي نفس
+
+377
+00:28:12,060 --> 00:28:16,340
+الفكرة ماتغير اللون فساعتها هضيف reagent c reagent
+
+378
+00:28:16,340 --> 00:28:22,980
+c إذا بعد إضافة reagent cإذا اتحول اللون للون red
+
+379
+00:28:22,980 --> 00:28:27,720
+يبقى هاي معناه انه البكتيريا ما عندها الانزيم هاي
+
+380
+00:28:27,720 --> 00:28:32,980
+النتيجة ال negative هاي النتيجة ال برضه ملخص ضفت
+
+381
+00:28:32,980 --> 00:28:39,800
+زراعة بكتيريا في ال media in ال nitrate ضفت
+
+382
+00:28:39,800 --> 00:28:43,880
+reagent a و reagent b بقولي لو ضفت reagent a و b و
+
+383
+00:28:43,880 --> 00:28:47,760
+اتحول اللون للون أحمر معناه هاي النتيجة positive
+
+384
+00:28:48,610 --> 00:28:52,390
+لكن إذا ما تغير اللون هضيف ال zinc powder إذا بعد
+
+385
+00:28:52,390 --> 00:28:56,070
+إضافة ال zinc powder ما تغير اللون هذا معناه إنه
+
+386
+00:28:56,070 --> 00:28:59,410
+عملت complete reduction أو اتحول ال nitrate ل
+
+387
+00:28:59,410 --> 00:29:03,790
+ammonia يبقى high في حال كانت positive لكن الصورة
+
+388
+00:29:03,790 --> 00:29:11,210
+الأبل في حال كان negativeهي نفس الفكرة بقوللي انه
+
+389
+00:29:11,210 --> 00:29:18,170
+طبعا بعد ما اضفت ال reagent A وB هانضفت اول شئ
+
+390
+00:29:18,170 --> 00:29:21,690
+طبعا زراعتي البكتيريا ضفت reagent A وB ما تغير
+
+391
+00:29:21,690 --> 00:29:25,590
+اللون هانتغير اللون للون Red يبقى هاي نتيجة
+
+392
+00:29:25,590 --> 00:29:29,070
+positive هاني نتيجة ما بنحكي negative بنضيف ال
+
+393
+00:29:29,070 --> 00:29:32,970
+zinc powder فدول التيوبين ضفت ال zinc powder على
+
+394
+00:29:32,970 --> 00:29:36,590
+ال tube هاي اللي طلعت colorless بعد إضافة reagent
+
+395
+00:29:36,590 --> 00:29:41,910
+A وBلحظنا إنه ما تغير اللون فبالتالي هذا معناه إنه
+
+396
+00:29:41,910 --> 00:29:44,590
+البكتيريا عملت complete reduction بما إنه عملت
+
+397
+00:29:44,590 --> 00:29:48,130
+complete reduction يبقى هي positive للإنزيم لو
+
+398
+00:29:48,130 --> 00:29:51,630
+اتحول اللون للون أحمر قلنا إنه هذه النتيجة معناها
+
+399
+00:29:51,630 --> 00:29:55,810
+negative ما عندها الإنزيم لكن تحول اللون الأحمر
+
+400
+00:29:55,810 --> 00:30:00,310
+نتيجة إنه الـ Zinc powder عمل reducing للـ Nitrate
+
+401
+00:30:00,310 --> 00:30:03,610
+اللي موجود في ال media لم يصل له أي استهلاك اتحول
+
+402
+00:30:03,610 --> 00:30:07,770
+ل Nitrateأتفعل مع agent A و B فعطاني اللون الأحمر
+
+403
+00:30:07,770 --> 00:30:13,750
+هاي نفس الفكرة هاي ال media مش مزروعة ك control
+
+404
+00:30:13,750 --> 00:30:18,770
+هان بدون قبل ما أضيف اللي هو ال zinc powder اتحول
+
+405
+00:30:18,770 --> 00:30:22,490
+اللون للون red يبقى هي positive هان ضفت ال zinc
+
+406
+00:30:22,490 --> 00:30:25,350
+powder بعد إضافة ال zinc powder كانت colorless
+
+407
+00:30:25,350 --> 00:30:29,070
+يبقى هي positive بعد ما ضفت ال zinc powder اتحول
+
+408
+00:30:29,070 --> 00:30:34,200
+اللون للون red يبقى هي negativeبقول لي في تلاتة
+
+409
+00:30:34,200 --> 00:30:39,320
+أمثلة على البكتيريا بالنسبة لفحص النيترات
+
+410
+00:30:39,320 --> 00:30:43,320
+reduction test كيف بدي أنا أفرق بينهم قلنا الـ
+
+411
+00:30:43,320 --> 00:30:46,380
+Staphylococcus epidermidis ما عندها القدرة أنها
+
+412
+00:30:46,380 --> 00:30:50,600
+تستخدم النيترات as terminal electron acceptor
+
+413
+00:30:50,600 --> 00:30:54,780
+فبالتالي مش هتقدر تختزل النيترات لنيترا تبقى هي
+
+414
+00:30:54,780 --> 00:30:56,980
+negative لـ Staphylococcus epidermidis negative
+
+415
+00:30:56,980 --> 00:31:01,350
+للفحصالـ E.coli وكل عقلة الـ Enterobacteriasi
+
+416
+00:31:01,350 --> 00:31:06,330
+بتختزل للنيترات فقط لـ Nitrite فبالتالي بعد إضافة
+
+417
+00:31:06,330 --> 00:31:10,650
+Reagent A وB كل عقلة الـ Enterobacteriasi هيتحول
+
+418
+00:31:10,650 --> 00:31:15,590
+لونها لون ال media للون أحمر الصدومونس قلنا إنه هي
+
+419
+00:31:15,590 --> 00:31:18,350
+تعتبر بتعمل Complete Reduction فبالتالي بعد ما
+
+420
+00:31:18,350 --> 00:31:22,750
+أضيف Reagent A وB هيصير عندي مش هيصير تفاعل هيكون
+
+421
+00:31:22,750 --> 00:31:25,730
+colorless هذا لا يعني إنه نتيجة negative عشان هيك
+
+422
+00:31:25,730 --> 00:31:29,060
+بضيف ال zinc powderإذا ضلت colorless معناه إنه
+
+423
+00:31:29,060 --> 00:31:32,080
+عملت complete reduction الـ pseudomonas
+
+424
+00:31:32,080 --> 00:31:36,980
+fluorescence قلنا إن عندها القدرة إنها تفرز
+
+425
+00:31:36,980 --> 00:31:40,860
+pigment diffusable yellow green pigment بتفرز
+
+426
+00:31:40,860 --> 00:31:45,180
+pigment diffusable قلنا soluble بتقدر إنها اتلون
+
+427
+00:31:45,180 --> 00:31:49,810
+الكوني وكمان الطبقممكن ان انا اشوفها fluorescence
+
+428
+00:31:49,810 --> 00:31:54,910
+in the ultraviolet light في ال UV بتعمل reduce
+
+429
+00:31:54,910 --> 00:32:01,070
+للنيتريات completely هتعمل completely reduction
+
+430
+00:32:01,070 --> 00:32:04,750
+complete reduction للنيتريات ل molecular nitrogen
+
+431
+00:32:04,750 --> 00:32:09,390
+عشان هيك لما اضيف reagent A و B مش هيتحول اللون
+
+432
+00:32:09,390 --> 00:32:13,900
+للون أحمر في حال كان البكتيريا pseudomonasهنا
+
+433
+00:32:13,900 --> 00:32:17,480
+بقولي دي في بعض ال safety rules عند استخدام
+
+434
+00:32:17,480 --> 00:32:21,680
+reagent اللي هو فحص النيترات reaction test قولي
+
+435
+00:32:21,680 --> 00:32:25,760
+reagent بيه اللي هو ال diet مثل نفط القمعين يعتبر
+
+436
+00:32:25,760 --> 00:32:30,570
+carcinogenic عشان هيكطبعا بتلبسي ال gloves بتحاولي
+
+437
+00:32:30,570 --> 00:32:36,650
+أنك تبعدي عن جلدك هذه المواد و كمان انك تستنشق هذه
+
+438
+00:32:36,650 --> 00:32:40,470
+المواد كمان ال reagent is sulfonylic acid يعتبر
+
+439
+00:32:40,470 --> 00:32:46,990
+كوزاتك مواد حارقة طبعا avoid skin contact بحاول
+
+440
+00:32:46,990 --> 00:32:50,730
+أني أبعده عن الجلد و عن إيدي و ما أني أنا أستنشقه
+
+441
+00:32:51,070 --> 00:32:55,950
+كمان أيضا عند استخدام الزنك باودر بيقوللي ممنوع
+
+442
+00:32:55,950 --> 00:33:00,410
+انك تستنشقيها او انه تلمس جلدكممنوع تستخدمي الـ
+
+443
+00:33:00,410 --> 00:33:03,210
+mouth by beating في فحص الـ Nitrate Reduction Test
+
+444
+00:33:03,210 --> 00:33:08,210
+لأن كل ال reagent اللي بنستخدمها تعتبر طبعا خطيرة،
+
+445
+00:33:08,210 --> 00:33:11,990
+هيك من جون خلصنا الفحص ال Nitrate Reduction Test
+
+446
+00:33:11,990 --> 00:33:17,930
+وخلصنا شعب ترب التلات فحوصات ال Ureas و ال Endol و
+
+447
+00:33:17,930 --> 00:33:21,410
+ال Nitrate Reduction Test السلام عليكم و رحمة الله
+
+448
+00:33:21,410 --> 00:33:22,210
+وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/XZSMgPWLPeQ.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/XZSMgPWLPeQ.srt
new file mode 100644
index 0000000000000000000000000000000000000000..eb66c1f979f05d78ebe4eccf94c85b529b0bc5bf
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/XZSMgPWLPeQ.srt
@@ -0,0 +1,967 @@
+1
+00:00:00,390 --> 00:00:04,130
+السلام عليكم ورحمة الله وبركاته، يعطيكم العافية.
+
+2
+00:00:04,130 --> 00:00:07,130
+هذا اللقاء سيكون معملنا بعنوان introduction to the
+
+3
+00:00:07,130 --> 00:00:10,910
+oil emerging compound microscope. هنتعرف على ال
+
+4
+00:00:10,910 --> 00:00:14,370
+light compound microscope، على المجهر الضوئي المركب،
+
+5
+00:00:14,760 --> 00:00:18,700
+استخدامات هذا المجهر، وأجزائه، ووظيفة كل جزء
+
+6
+00:00:18,700 --> 00:00:22,740
+بالتفصيل. سنتعرف كمان على بعض النقاط المهمة بالنسبة
+
+7
+00:00:22,740 --> 00:00:26,480
+للـ light compound microscope. آخر جزئية سنتكلم كيف
+
+8
+00:00:26,480 --> 00:00:31,660
+أنا بدي أحفظ، أو أني أحافظ على الـ microscope، وأني
+
+9
+00:00:31,660 --> 00:00:36,760
+أنظفه بعد ما أخلص من شغلي. في الـ introduction عرفنا
+
+10
+00:00:36,760 --> 00:00:39,420
+الـ microbiology، وقلنا it is the study of
+
+11
+00:00:39,420 --> 00:00:43,500
+microorganisms that cannot be seen by the naked eye. من
+
+12
+00:00:43,500 --> 00:00:46,220
+الـ microorganisms اللي احنا هنتعامل معها في هذا
+
+13
+00:00:46,220 --> 00:00:50,800
+المعمل، اللي هو البكتيريا. حجم البكتيريا بيتراوح، و
+
+14
+00:00:50,800 --> 00:00:54,000
+معظم حجم البكتيريا بيتراوح ما بين نصف لإثنين micro
+
+15
+00:00:54,000 --> 00:00:58,560
+meter. micrometer، كثير حجم صغير، عشان إني أنا أقدر
+
+16
+00:00:58,560 --> 00:01:01,920
+أشوفها، بحتاج الـ microscope. فأنا بدي أعرف ال
+
+17
+00:01:01,920 --> 00:01:05,800
+microscopy. Microscopy is the technology of making
+
+18
+00:01:05,800 --> 00:01:09,000
+very small things visible to the human eye. هو
+
+19
+00:01:09,000 --> 00:01:12,100
+التقنية المستخدمة في تكبير الأشياء الصغيرة التي
+
+20
+00:01:12,100 --> 00:01:16,340
+لا ترى بالعين المجردة. used in the diagnosis of
+
+21
+00:01:16,340 --> 00:01:19,640
+many diseases. يستخدم الميكروسكوب في تشخيص العديد من
+
+22
+00:01:19,640 --> 00:01:24,120
+الأمراض. على سبيل المثال، الـ cancer، في معمل الـ
+
+23
+00:01:24,120 --> 00:01:29,320
+histopathology، في معمل الأنسجة، كنتوا بتاخدوا ال
+
+24
+00:01:29,320 --> 00:01:33,220
+biopsy، ويتقطعوها، ويمرّوها بمراحل، وبعدين
+
+25
+00:01:33,220 --> 00:01:36,920
+تصبغوها، وبعد كده تشوفوها تحت الـ microscope. كنتوا
+
+26
+00:01:36,920 --> 00:01:41,260
+بتتأكدوا من عدم وجود نمو غير طبيعي للخلايا، فبالتالي
+
+27
+00:01:41,260 --> 00:01:45,100
+كنتوا تقدروا تشخصوا هل عند المريض cancer أو لا.
+
+28
+00:01:45,100 --> 00:01:51,630
+used for soil, insect, and rock. تستخدم في التربة، وفي
+
+29
+00:01:51,630 --> 00:01:55,210
+الـ insects، الحشرات. لو أنا بدي أشوف حشرة، أشوف
+
+30
+00:01:55,210 --> 00:01:59,530
+التفاصيل بتاعتها، بشوفها تحت الـ microscope. بالنسبة
+
+31
+00:01:59,530 --> 00:02:04,150
+للسخور، المجاهر بنستخدمها في تحليل التركيب المعدني
+
+32
+00:02:04,150 --> 00:02:08,190
+لبعض السخور. used in pathological laboratories and
+
+33
+00:02:08,190 --> 00:02:12,010
+medical. بنستخدم الـ microscope في المختبرات طبعاً
+
+34
+00:02:12,010 --> 00:02:16,540
+أكيد، مافيش أي مختبر ما فيه microscope. لو أنا
+
+35
+00:02:16,540 --> 00:02:20,880
+مثلاً بدي أعمل فيلم للدم، بصفغه بصبغات خاصة، وبشوفه
+
+36
+00:02:20,880 --> 00:02:24,960
+تحت الـ microscope. من خلاله هيتم تشخيص العديد من
+
+37
+00:02:24,960 --> 00:02:28,560
+أمراض الدم المختلفة، من الأنيميا واللوكيميا.
+
+38
+00:02:28,560 --> 00:02:32,520
+industrial microscope used for metals and various
+
+39
+00:02:32,520 --> 00:02:37,080
+kinds of measurements. نستخدم الـ microscope للمعادن،
+
+40
+00:02:37,480 --> 00:02:41,100
+educational microscope used for research. أكيد، ل
+
+41
+00:02:41,100 --> 00:02:46,860
+هدف تعليمي، وأيضاً في مجال الأبحاث، وفي رؤية تركيب
+
+42
+00:02:46,860 --> 00:02:51,540
+الخلايا النباتية والحيوانية، وغيرها من التطبيقات.
+
+43
+00:02:51,540 --> 00:02:56,220
+bacteria is one of the smallest microorganisms that
+
+44
+00:02:56,220 --> 00:02:58,540
+cannot be seen with the naked eye. اتفقنا أن
+
+45
+00:02:58,540 --> 00:03:01,260
+البكتيريا من الكائنات الدقيقة التي لا ترى بالعين
+
+46
+00:03:01,260 --> 00:03:01,980
+المجردة.
+
+47
+00:03:04,330 --> 00:03:09,850
+معظم حجم البكتيريا يتراوح بين نصف لإثنين ميكرومتر.
+
+48
+00:03:09,850 --> 00:03:14,110
+وهذا اتفقنا عليه في أول المعمل، لذلك يحتاج الناس
+
+49
+00:03:14,110 --> 00:03:17,530
+لدراسة البكتيريا، للوقت السريع، باستخدام ميكروسكوب
+
+50
+00:03:17,530 --> 00:03:22,050
+لرؤيتها. عشان هيك أنا بحتاج الميكروسكوب حتى أني أنا
+
+51
+00:03:22,050 --> 00:03:27,170
+أكبرها عشان أقدر أشوفها. لكن أكيد إحنا قلنا إن معظم
+
+52
+00:03:27,170 --> 00:03:30,570
+حجم البكتيريا بيتراوح ما بين نصف لإثنين ميكرومتر.
+
+53
+00:03:31,070 --> 00:03:34,630
+أكيد، لكل قاعدة شواذ. في علم الـ microbiology، مافيش
+
+54
+00:03:34,630 --> 00:03:39,050
+شيء absolute، مافيش شيء مطلق، كل شيء له استثناء. من
+
+55
+00:03:39,050 --> 00:03:42,410
+هذه الاستثناءات، أن أنا في عندي giant bacteria اسمها
+
+56
+00:03:42,410 --> 00:03:47,750
+Thiomargarita namibiensis. هذه البكتيريا تعتبر giant
+
+57
+00:03:47,750 --> 00:03:51,970
+bacteria، أقدر أشوفها بالعين المجردة. بيتراوح حجم
+
+58
+00:03:51,970 --> 00:03:55,910
+هذه البكتيريا ما بين 1 من 10 ل 3 من 10 ملي متر. لو
+
+59
+00:03:55,910 --> 00:04:00,770
+أنا بدي أحولها لـ micrometer، بتروح ما بين 100 لـ 300
+
+60
+00:04:00,770 --> 00:04:04,630
+ميكرومتر في الـ diameter، وبعض الأحيان ممكن توصل
+
+61
+00:04:04,630 --> 00:04:10,870
+لـ 75 من 100 ملي متر، أي 750 ميكرومتر. يبقى جملة
+
+62
+00:04:10,870 --> 00:04:14,310
+"كل البكتيريا لا تُرى بالعين المجردة" ده غلط، لأنه أنا
+
+63
+00:04:14,310 --> 00:04:17,430
+في عندي، في استثناء، في عندي Thiomargarita namibiensis.
+
+64
+00:04:17,430 --> 00:04:20,730
+هناك أنواع مختلفة من الميكروسكوبات التي
+
+65
+00:04:20,730 --> 00:04:23,810
+تستخدم في علم الميكروبيولوجي، وقلنا إنه إحنا بنعرف
+
+66
+00:04:23,810 --> 00:04:27,250
+أنواع مختلفة من الميكروسكوبات. على سبيل المثال، أنا
+
+67
+00:04:27,250 --> 00:04:30,210
+في عندي الـ light microscope، يعني دي الـ light
+
+68
+00:04:30,210 --> 00:04:32,830
+compound microscope. يعني دي الـ fluorescent، الـ
+
+69
+00:04:32,830 --> 00:04:36,090
+electron microscope. كل هذه الميكروسكوبات أنا
+
+70
+00:04:36,090 --> 00:04:42,110
+بستخدمها في علم الـ microbiology. لكن in this lab
+
+71
+00:04:42,110 --> 00:04:44,890
+you will become familiar with the use of the light
+
+72
+00:04:44,890 --> 00:04:48,390
+compound microscope. فهذا المعمل سنتعرف على الـ
+
+73
+00:04:48,390 --> 00:04:51,590
+light compound microscope. الـ light compound
+
+74
+00:04:51,590 --> 00:04:54,270
+microscope، ليش أنا سميته بهذا الاسم؟ الـ light لأن
+
+75
+00:04:54,270 --> 00:04:58,520
+الضوء بيمر خلال الجسم. compound لأنه
+
+76
+00:04:58,520 --> 00:05:02,220
+بيتركب من عدستين: عدسة عينية (ocular lens)، وعدسة
+
+77
+00:05:02,220 --> 00:05:06,020
+شيئية (objective lens). and will compare the
+
+78
+00:05:06,020 --> 00:05:09,800
+relative size and shape of various microorganisms.
+
+79
+00:05:09,800 --> 00:05:13,740
+من خلاله أنا بتعرف على الحجم النسبي للبكتيريا،
+
+80
+00:05:13,740 --> 00:05:20,340
+وأيضاً أشكال الكائنات الدقيقة المختلفة. microscope
+
+81
+00:05:20,340 --> 00:05:23,680
+consists of four major parts. بقول لك الميكروسكوب
+
+82
+00:05:23,680 --> 00:05:28,470
+بيتكّون من أربعة مكونات أساسية. أول مكوّن عندنا اللي هو
+
+83
+00:05:28,470 --> 00:05:31,950
+الـ framework. الـ framework اللي هو إطار العمل.
+
+84
+00:05:31,950 --> 00:05:36,010
+إطار العمل بيتكون من ثلاثة أجزاء: arm, stage, base.
+
+85
+00:05:36,010 --> 00:05:40,350
+هنتكلم عنها بالتفصيل. عندي ثاني جزء من أجزاء الـ
+
+86
+00:05:40,350 --> 00:05:44,310
+microscope المهمة، الـ adjustment system، نظام
+
+87
+00:05:44,310 --> 00:05:48,090
+الضبط. نظام الضبط بيتكون من الـ fine adjustment، و
+
+88
+00:05:48,090 --> 00:05:51,230
+الـ coarse adjustment، اللي هو الضابط الصغير، و
+
+89
+00:05:51,230 --> 00:05:54,860
+الضابط الكبير.  الثالث جزء اللي هو الـ magnification،
+
+90
+00:05:54,860 --> 00:05:57,840
+التكبير، المسؤول عن التكبير في الـ microscope، اللي
+
+91
+00:05:57,840 --> 00:06:02,520
+هم العدسات: الـ ocular lens، العدسة العينية، و الـ
+
+92
+00:06:02,520 --> 00:06:06,500
+objective lens، العدسات الشيئية.  آخر جزء اللي هو الـ
+
+93
+00:06:06,500 --> 00:06:09,880
+lighting system، اللي هو نظام الإضاءة. فيه عندي الـ
+
+94
+00:06:09,880 --> 00:06:14,000
+condenser، اللي هو المكثف، اللي بيجمع الضوء، وإضافة
+
+95
+00:06:14,000 --> 00:06:17,680
+إلى diaphragm، اللي هو بتحكم في كمية الضوء المار عبر العين.
+
+96
+00:06:24,230 --> 00:06:26,990
+في هذه الـ slide، سنتكلم بالتفصيل عن أجزاء
+
+97
+00:06:26,990 --> 00:06:31,830
+الميكروسكوب، ووظيفة كل جزء. في عندي أول جزء، الـ
+
+98
+00:06:31,830 --> 00:06:36,030
+ocular lens، العدسات العينية. في عندي two ocular
+
+99
+00:06:36,030 --> 00:06:40,190
+lenses.  فدول العدسات العينية، شو وظيفتهم؟ remagnify
+
+100
+00:06:40,190 --> 00:06:44,670
+the image. بيعمل على إعادة تكبير الصورة. طيب مين
+
+101
+00:06:44,670 --> 00:06:49,810
+اللي بيعمل magnify؟ مين المسؤول الأساسي والأول عن
+
+102
+00:06:49,810 --> 00:06:54,010
+تكبير الصورة؟ نكمل الجملة: formed by the objective
+
+103
+00:06:54,010 --> 00:06:57,970
+lens. اللي بيتم تكبيرها بواسطة الـ objective lens.
+
+104
+00:06:57,970 --> 00:07:02,630
+يبقى الـ objective lens، اللي هي العدسات الشيئية، هي
+
+105
+00:07:02,630 --> 00:07:06,150
+الـ primary lens that magnifies the specimen. هي
+
+106
+00:07:06,150 --> 00:07:09,990
+العدسة الأولية اللي بتكبّر لي الصورة. طبعاً لأن هي
+
+107
+00:07:09,990 --> 00:07:13,910
+قريبة من العينة، فأكيد هتكون هي العدسة الأولية اللي
+
+108
+00:07:13,910 --> 00:07:19,180
+بتكبّر الصورة. remagnify. الـ ocular lens بتكبّر لي
+
+109
+00:07:19,180 --> 00:07:24,340
+بعدها، بتوصل الصورة للعدسة العينية، وبتكبّر لي إياها
+
+110
+00:07:24,340 --> 00:07:29,040
+حتى إني أنا أحصل بالنهاية على total magnification.
+
+111
+00:07:29,040 --> 00:07:31,800
+إيش يعني total magnification؟ هو حاصل ضرب ال
+
+112
+00:07:31,800 --> 00:07:35,020
+objective magnification، قوة تكبير العدسة الشيئية،
+
+113
+00:07:35,020 --> 00:07:38,840
+اللي أنا استخدمتها، مع قوة تكبير العدسة العينية،
+
+114
+00:07:38,840 --> 00:07:42,380
+الـ ocular magnification، اللي هي العشرة ×. قوة تكبير
+
+115
+00:07:42,380 --> 00:07:46,860
+العدسة العينية عشرة ×. لكن قوة تكبير العدسة الشيئية
+
+116
+00:07:46,860 --> 00:07:51,340
+في عندي أنا أربعة عدسات: أربعة، عشرة، أربعين، مائة. فهو
+
+117
+00:07:51,340 --> 00:07:55,080
+استخدمت ضربها في قوة تكبير العدسة العينية العشرة
+
+118
+00:07:55,080 --> 00:07:59,940
+×، حتى أحصل على الـ total magnification. بالنسبة
+
+119
+00:07:59,940 --> 00:08:05,140
+للعدسات الشيئية، العدسات الشيئية مربوطين بشيء اسمه
+
+120
+00:08:05,140 --> 00:08:11,910
+nosepiece. هذا هو الـ nosepiece، قرص دائري، أو جزء
+
+121
+00:08:11,910 --> 00:08:15,970
+دائري، بيحمل العدسات الشيئية. نتحكم فيه من خلال
+
+122
+00:08:15,970 --> 00:08:20,170
+التدوير، وبالتالي بيؤدي لـ تغيير قوة التكبير اللي أنا
+
+123
+00:08:20,170 --> 00:08:23,750
+بدي إياها: أربعة، أو عشرة، أو أربعين، أو مائة، حسب أنا
+
+124
+00:08:23,750 --> 00:08:30,810
+شو ما بدي. بالنسبة للـ body، نيجي للـ body. الـ body
+
+125
+00:08:30,810 --> 00:08:34,310
+هو اسم ثاني اللي هو الـ optical tube، الأنبوب
+
+126
+00:08:34,310 --> 00:08:38,250
+البصري. transmits the image from the objective lens
+
+127
+00:08:38,250 --> 00:08:42,670
+to the ocular lens using prisms. بقول لك بينقل الصورة
+
+128
+00:08:42,670 --> 00:08:46,570
+من الـ objective lens للـ ocular lens. ننتبه إنه مش
+
+129
+00:08:46,570 --> 00:08:49,950
+العكس، لأن إحنا قلنا أن الـ objective lens هي
+
+130
+00:08:49,950 --> 00:08:54,410
+المسؤولة عن التكبير بشكل أولي، لأن هي المسؤولة
+
+131
+00:08:54,410 --> 00:08:56,910
+الأولى عن التكبير. فبالتالي هو بينقل من ال
+
+132
+00:08:56,910 --> 00:09:02,240
+objective للـ ocular lens. الـ arm اللي هو الذراع،
+
+133
+00:09:02,240 --> 00:09:05,260
+الذراع، أنا بستخدمه لحمل الـ microscope، ونقله من
+
+134
+00:09:05,260 --> 00:09:09,960
+مكان لآخر. بالنسبة للـ stage، الـ stage هي عبارة
+
+135
+00:09:09,960 --> 00:09:14,000
+عن سطح، إحنا شايفين سطح مستوي، يوجد تحت العدسات
+
+136
+00:09:14,000 --> 00:09:17,920
+الشيئية، مخصص لوضع الشريحة عليه. holds the microscope
+
+137
+00:09:17,920 --> 00:09:22,100
+slide in position. مخصص لوضع الـ slide، أو الشريحة
+
+138
+00:09:22,100 --> 00:09:27,210
+عليه. فيه عندي ملاقط clips، بتساعد في تثبيت الشريحة
+
+139
+00:09:27,210 --> 00:09:31,190
+على الـ stage، وضمان عدم تحركها أثناء الشغل. فيه موجود
+
+140
+00:09:31,190 --> 00:09:34,710
+تحت اللي هو الـ stage، شيء اسمه الـ condenser، اللي هو
+
+141
+00:09:34,710 --> 00:09:38,890
+المكثف. مش وظيفته focus light through the specimen?
+
+142
+00:09:38,890 --> 00:09:44,450
+بيقول لك بيجمع، أو بيركز الضوء المتجهة نحو
+
+143
+00:09:44,450 --> 00:09:49,830
+الشريحة، وبعدين هيمر عبر العدسات الشيئية، طبعاً.
+
+144
+00:09:51,530 --> 00:09:54,230
+الـ condenser فيه تحته diaphragm، يعني الـ
+
+145
+00:09:54,230 --> 00:09:57,270
+diaphragm، مسؤول عن control of the amount of
+
+146
+00:09:57,270 --> 00:10:01,290
+light entering the condenser. بتحكم بكمية الضوء
+
+147
+00:10:01,290 --> 00:10:05,930
+الواصلة للـ condenser، وبعدين هتوصل للعينة، ثم العدسات
+
+148
+00:10:05,930 --> 00:10:10,950
+الشيئية. في عندي أكيد مصدر للضوء. آخر شيء في عندي
+
+149
+00:10:10,950 --> 00:10:16,210
+ضابطين على جانبي المجهر: ضابط كبير (course focusing
+
+150
+00:10:16,210 --> 00:10:20,890
+knob)، وضابط صغير (fine focusing knob). شو وظيفة الـ
+
+151
+00:10:20,890 --> 00:10:24,610
+course focusing knob؟ وظيفته move the stage up
+
+152
+00:10:24,610 --> 00:10:27,210
+and down to focus the image. بقول لك بحرك الـ
+
+153
+00:10:27,210 --> 00:10:31,330
+stage لفوق وللتحت، حتى إنه يثبت الصورة. مش يعني
+
+154
+00:10:31,330 --> 00:10:35,410
+focus the image، نفس معناه أنه أنا عشان أقدر أجيب
+
+155
+00:10:35,410 --> 00:10:38,890
+الـ field، عشان أقدر أجيب الـ field، بحرك الـ stage
+
+156
+00:10:38,890 --> 00:10:42,610
+لأعلى أو لأسفل، حتى أقدر أجيبها في الصورة اللي أنا
+
+157
+00:10:42,610 --> 00:10:46,680
+بدي إياها. هيك أنا معناها قدرت أجيب الـ field. الـ fine
+
+158
+00:10:46,680 --> 00:10:50,240
+focusing knob، هو الضابط الصغير. وظيفته اللي هو
+
+159
+00:10:50,240 --> 00:10:54,000
+الـ clarity، الوضوح، حتى إنه يزيد وضوح الصورة. أنا
+
+160
+00:10:54,000 --> 00:10:58,480
+بستخدمه بعد ما أجيب الـ field تمام. جبت الـ field، لو انتقلت
+
+161
+00:10:58,480 --> 00:11:03,180
+لعدسة high power، لقوة تكبير أعلى، بلاقي الصورة
+
+162
+00:11:03,180 --> 00:11:07,020
+مغبشة. فأنا بروح بلعب بالـ fine عشان إنه تزيد
+
+163
+00:11:07,020 --> 00:11:10,420
+وضوح الصورة، تزيد الـ clarity. الـ base هي
+
+164
+00:11:10,420 --> 00:11:15,040
+الركيزة الأساسية للمجهر، واللي هي بتحمل الـ
+
+165
+00:11:15,040 --> 00:11:19,720
+microscope. هذا بالنسبة لأجزاء الـ microscope.
+
+166
+00:11:19,720 --> 00:11:23,960
+تعرفنا على وظيفة
+
+167
+00:11:23,960 --> 00:11:27,800
+كل جزء، لكن سنتكلم بالتفصيل عن كل جزء في الـ
+
+168
+00:11:27,800 --> 00:11:29,240
+slides اللي جاية.
+
+169
+00:11:38,830 --> 00:11:42,710
+تكلمنا إن الميكروسكوب بتتكون من أربعة أجزاء، من ضمن
+
+170
+00:11:42,710 --> 00:11:45,370
+هذه الأجزاء، أول شيء اللي هو الـ framework. الـ
+
+171
+00:11:45,370 --> 00:11:48,450
+framework بتتكون من ثلاثة أجزاء: الـ base اللي هي
+
+172
+00:11:48,450 --> 00:11:51,850
+القاعدة، و الـ arm اللي هو الذراع، من خلال اللي قلنا
+
+173
+00:11:51,850 --> 00:11:55,590
+بحمل الميكروسكوب وبنقله من مكان لآخر، و الـ
+
+174
+00:11:55,590 --> 00:11:58,730
+mechanical stage. طب أنا لو بدي أعرف شو هي الـ
+
+175
+00:11:58,730 --> 0
+
+223
+00:15:31,690 --> 00:15:35,690
+The adjustment knob is used for improving the clarity
+
+224
+00:15:35,690 --> 00:15:39,610
+حتى إنه يزيد وضوح الصورة، especially when viewing
+
+225
+00:15:39,610 --> 00:15:43,690
+under high power، خصوصًا لما أنا أستخدم الـHigh
+
+226
+00:15:43,690 --> 00:15:47,970
+Power. مش قصدي بهذه الجملة خلال الشغل العملي لما
+
+227
+00:15:47,970 --> 00:15:51,770
+أنا أجيب الـField على 4X، وبعدين أروح أحول على 40X
+
+228
+00:15:51,770 --> 00:15:55,110
+أو على 100 اللي هي الـHigh Power، بلاقي الصورة
+
+229
+00:15:55,110 --> 00:15:59,950
+مغبشة، فعشان أنا أزيد وضوح الصورة، يتكون improving
+
+230
+00:15:59,950 --> 00:16:02,850
+the clarity of the image، بروح بحرك الـFine
+
+231
+00:16:02,850 --> 00:16:06,930
+Adjustment شوية، فبالتالي هيك بتكون الصورة واضحة.
+
+232
+00:16:06,930 --> 00:16:09,550
+وفي بعض الأمور الثانية اللي ممكن كمان إن أنا أتحكم
+
+233
+00:16:09,550 --> 00:16:14,770
+فيها، نتكلم عنها في الـ «سلايدات» اللي جاية، فأنا هان
+
+234
+00:16:14,770 --> 00:16:17,390
+بأستخدم فقط الـ «fine adjustment»، جبت الـ «field»
+
+235
+00:16:17,390 --> 00:16:21,110
+ممنوع أستخدم الـ «coarse adjustment»، خلاص فقط بحرك
+
+236
+00:16:21,110 --> 00:16:25,410
+الـ «fine adjustment» عشان تزيد وضوح الصورة، ممنوع
+
+237
+00:16:25,410 --> 00:16:28,750
+أمسك الـ «coarse adjustment». هذا بالنسبة لـ
+
+238
+00:16:28,750 --> 00:16:32,770
+«coarse» والـ «fine adjustment knob». هاي مكونات
+
+239
+00:16:32,770 --> 00:16:37,290
+الـ «adjustment system». نيجي لآخر مكون اللي هو الـ
+
+240
+00:16:37,290 --> 00:16:39,930
+«interpupillary adjustment»
+
+241
+00:16:41,930 --> 00:16:45,210
+بشهدها الـ Inter-pupillary adjustment to control
+
+242
+00:16:45,210 --> 00:16:48,230
+the distance between the ocular lens to adopt
+
+243
+00:16:48,230 --> 00:16:52,910
+the distance between viewer's eye. قلنا إن المسافة
+
+244
+00:16:52,910 --> 00:16:58,750
+بين عينَي أي شخص بتختلف، فبالتالي عشان أتحكم في
+
+245
+00:16:58,750 --> 00:17:03,170
+المسافة ما بين العدسات العينية حتى إنها تكون
+
+246
+00:17:03,170 --> 00:17:07,610
+مناسبة لمسافة ما بين عينَي الراصد. هذه أنا بستخدم
+
+247
+00:17:07,610 --> 00:17:12,950
+الـ Inter-pupillary adjustment. الـ Eye-Base Lens هي
+
+248
+00:17:12,950 --> 00:17:17,430
+نفسها الـ Ocular Lens. ممكن هو الـ Spread apart
+
+249
+00:17:17,430 --> 00:17:22,290
+ممكن أبعدهم عن بعض أو Get closer together to fit
+
+250
+00:17:22,290 --> 00:17:28,130
+each individual. أو ممكن أقربهم حسب شخص بيرتاح، حسب
+
+251
+00:17:28,130 --> 00:17:32,690
+ما بيرتاح. ممكن أن أنا أبعدهم أو أقربهم. هذا بالنسبة
+
+252
+00:17:32,690 --> 00:17:34,410
+للـ Adjustment.
+
+253
+00:17:42,630 --> 00:17:45,930
+ثالث مكوّن من مكونات الميكروسكوب، الـ Magnification.
+
+254
+00:17:45,930 --> 00:17:48,590
+قلنا المسؤول عن الـ Magnification العدسات الـ
+
+255
+00:17:48,590 --> 00:17:51,530
+Ocular Lens، العدسات العينية، الـ IBIS اللي هي اسم
+
+256
+00:17:51,530 --> 00:17:54,910
+ثاني، والـ Objective Lens اللي هي العدسات الشيئية.
+
+257
+00:17:54,910 --> 00:17:59,510
+الـ Ocular Lens or الـ IBIS is used for viewing.
+
+258
+00:17:59,510 --> 00:18:04,510
+العدسات العينية تستخدم للرؤية. Revolving Nosepiece
+
+259
+00:18:04,510 --> 00:18:07,810
+contains Objective Lenses that are used to magnify.
+
+260
+00:18:07,810 --> 00:18:12,270
+قلنا إن في أندي أنا هان إيش اسمه Nosepiece. هذا هو
+
+261
+00:18:12,270 --> 00:18:16,770
+الـ Nosepiece، قرص دائري من خلاله أنا بدور العدسات
+
+262
+00:18:16,770 --> 00:18:20,770
+الشيئية، حسب أنا شو بدي. بحرك و بختار العدسة الشيئية
+
+263
+00:18:20,770 --> 00:18:25,210
+اللي أنا بدي أستخدمها. بقول لك هذا اسمه في العربية
+
+264
+00:18:25,210 --> 00:18:28,650
+فيه اسم اللي هو الطاحونة، بيحتوي، بيحمل العدسات
+
+265
+00:18:28,650 --> 00:18:33,220
+الشيئية. شو وظيفة العدسات؟ تستخدم لـ Magnify، لتكبير
+
+266
+00:18:33,220 --> 00:18:36,880
+الصورة، بالاتحاد مع الـ Ocular Lens. واتفقنا إنه
+
+267
+00:18:36,880 --> 00:18:39,560
+بالنهاية أنا بحصل على Total Magnification من حاصل
+
+268
+00:18:39,560 --> 00:18:41,980
+ضرب الـ Magnification للعدسات العينية في الـ
+
+269
+00:18:41,980 --> 00:18:45,720
+Magnification للعدسات الشيئية. The objective of
+
+270
+00:18:45,720 --> 00:18:48,760
+the microscope is not just to increase magnification
+
+271
+00:18:48,760 --> 00:18:53,440
+but to do so while attaining sufficient resolution.
+
+272
+00:18:54,450 --> 00:18:57,230
+في هذه الجملة، في أول معمل الـ Microscope، قلنا إن
+
+273
+00:18:57,230 --> 00:19:01,150
+الـ Microscope هو تقنية تجعل الأشياء الصغيرة يمكن
+
+274
+00:19:01,150 --> 00:19:05,070
+رؤيتها بالعين المجردة من خلال تكبيرها. بالجملة هذه
+
+275
+00:19:05,070 --> 00:19:08,690
+بقول إن أنا مش هدفي من الـ Microscope فقط إن أنا
+
+276
+00:19:08,690 --> 00:19:12,750
+أكبر الأشياء، كمان لازم أحصل على دقة عالية للصورة،
+
+277
+00:19:12,750 --> 00:19:16,830
+تكون واضحة. على سبيل المثال، لو وقت كتير بنزل صورة
+
+278
+00:19:16,830 --> 00:19:20,090
+على الجوال، بروح أنا بعمل zoom، بكبر عشان أشوف بعض
+
+279
+00:19:20,090 --> 00:19:25,980
+التفاصيل، ما بقدر أشوفها بشكل واضح. فبالتالي هان ممكن
+
+280
+00:19:25,980 --> 00:19:30,640
+تكون الصورة مكبرة، ولكن مش واضحة. نطبق الموضوع على
+
+281
+00:19:30,640 --> 00:19:33,980
+الـ Microscope، لما أنت تجيبي الـ Field على 10X، و
+
+282
+00:19:33,980 --> 00:19:38,180
+تروحي على 40X، الـ High Power، هتلاحظي أن الصورة
+
+283
+00:19:38,180 --> 00:19:41,780
+مغبشة ومش واضحة. عشان تحصلي على Resolution عالي،
+
+284
+00:19:41,780 --> 00:19:45,580
+على دقة عالية، بتروحي بتحركي الـ Fine Adjustment
+
+285
+00:19:45,580 --> 00:19:50,710
+اللي هو الضابط الصغير، حتى تحصلي على دقة عالية. وكمان
+
+286
+00:19:50,710 --> 00:19:54,790
+ممكن أنه نتحكم في أمور ثانية، هنتكلم عنها في الـ
+
+287
+00:19:54,790 --> 00:19:58,770
+Slides اللي جاي. يبقى مش بس الهدف من الـ Microscope
+
+288
+00:19:58,770 --> 00:20:02,270
+أني أنا أكبر، لازم أني أنا أحصل على دقة عالية من
+
+289
+00:20:02,270 --> 00:20:04,210
+خلال التحكم في بعض الأمور.
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/XZSMgPWLPeQ_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/XZSMgPWLPeQ_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..296769551c38e4583765ed94bb19da606e288a66
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/XZSMgPWLPeQ_raw.json
@@ -0,0 +1 @@
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"end": 783.43, "text": "هدولا الـ Stage knob control أنا بحرك فيهم الـ Stage لليمين و لا اليسار و لا الأمام و لا الخلف طيب متى أنا بحتاج هذا الاشياء على سبيل المثال أنا في عندي slide و بدي أشوف كل هذه الشريحة بدي أمر على كل هذه الشريحة فعشان أنا ما أضيع ولا field بروح بمشي زيجزاج بمشي بهذه الطريقة حتى إن أنا أضمن إن أنا مريت على كل الشريحة", "tokens": [3224, 3215, 12610, 995, 2423, 39184, 25907, 26759, 1969, 41850, 4724, 5016, 31747, 8978, 16095, 2423, 39184, 25907, 5296, 20292, 2304, 9957, 4032, 20193, 45595, 3794, 9640, 4032, 20193, 16247, 2304, 10943, 4032, 20193, 33962, 46538, 23032, 1829, 3555, 44650, 7578, 41850, 4724, 33753, 26108, 23758, 2423, 33599, 1829, 16606, 15844, 8608, 21292, 1211, 9673, 12984, 6027, 41850, 8978, 43242, 1829, 4137, 4032, 4724, 16254, 5551, 8592, 38688, 28242, 29538, 25124, 16572, 5016, 3660, 4724, 16254, 5551, 29973, 15844, 28242, 29538, 25124, 16572, 5016, 3660, 6156, 3615, 8592, 7649, 41850, 19446, 5551, 11242, 40228, 49429, 2519, 4724, 32887, 5016, 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772.53, "word": " الشريحة", "probability": 0.9964599609375}, {"start": 772.53, "end": 773.03, "word": " فعشان", "probability": 0.9466552734375}, {"start": 773.03, "end": 773.29, "word": " أنا", "probability": 0.7783203125}, {"start": 773.29, "end": 773.45, "word": " ما", "probability": 0.6962890625}, {"start": 773.45, "end": 773.87, "word": " أضيع", "probability": 0.8987630208333334}, {"start": 773.87, "end": 774.09, "word": " ولا", "probability": 0.91552734375}, {"start": 774.09, "end": 774.41, "word": " field", "probability": 0.74560546875}, {"start": 774.41, "end": 774.79, "word": " بروح", "probability": 0.9479166666666666}, {"start": 774.79, "end": 775.15, "word": " بمشي", "probability": 0.9697265625}, {"start": 775.15, "end": 775.73, "word": " زيجزاج", "probability": 0.60263671875}, {"start": 775.73, "end": 778.29, "word": " بمشي", "probability": 0.974365234375}, {"start": 778.29, "end": 778.73, "word": " بهذه", "probability": 0.890380859375}, {"start": 778.73, "end": 779.23, "word": " الطريقة", "probability": 0.9894205729166666}, {"start": 779.23, "end": 779.55, "word": " حتى", "probability": 0.92578125}, {"start": 779.55, "end": 779.73, "word": " إن", "probability": 0.299072265625}, {"start": 779.73, "end": 779.95, "word": " أنا", "probability": 0.537109375}, {"start": 779.95, "end": 780.35, "word": " أضمن", "probability": 0.97216796875}, {"start": 780.35, "end": 780.55, "word": " إن", "probability": 0.53369140625}, {"start": 780.55, "end": 780.87, "word": " أنا", "probability": 0.92041015625}, {"start": 780.87, "end": 781.45, "word": " مريت", "probability": 0.86474609375}, {"start": 781.45, "end": 781.65, "word": " على", "probability": 0.9541015625}, {"start": 781.65, "end": 782.15, "word": " كل", "probability": 0.990234375}, {"start": 782.15, "end": 783.43, "word": " الشريحة", "probability": 0.9949951171875}], "temperature": 1.0}, {"id": 31, "seek": 81494, "start": 785.58, "end": 814.94, "text": "طبعا فانا هان هستخدم ال stage knob control حتى انها تساعدني في الحركة فعشان انا اول شي هبدأ بالحركة للامام فعشان انا اتحرك للامام هستخدم ال top knob is to move the forward and backward هيحركلي ال stage للامام و للخلف انا بدي اتحرك للامام هستخدم ال top knob فبدي ارجع في field لأ بدي ارجع اشوفه بقى استخدم برضه ال top knob بحركليها للامام و للخلف", "tokens": [9566, 3555, 3615, 995, 6156, 7649, 995, 8032, 7649, 8032, 14851, 9778, 40448, 2423, 3233, 26759, 1969, 11331, 49975, 16472, 11296, 6055, 3794, 995, 22488, 22653, 8978, 21542, 31747, 3660, 6156, 3615, 8592, 7649, 1975, 8315, 1975, 12610, 44049, 8032, 44510, 10721, 20666, 5016, 31747, 3660, 24976, 10943, 10943, 6156, 3615, 8592, 7649, 1975, 8315, 1975, 2655, 5016, 31747, 24976, 10943, 10943, 8032, 14851, 9778, 40448, 2423, 1192, 26759, 307, 281, 1286, 264, 2128, 293, 23897, 39896, 5016, 31747, 20292, 2423, 3233, 24976, 10943, 10943, 4032, 24976, 9778, 46538, 1975, 8315, 4724, 16254, 1975, 2655, 5016, 31747, 24976, 10943, 10943, 8032, 14851, 9778, 40448, 2423, 1192, 26759, 6156, 3555, 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"end": 1199.05, "word": " أني", "probability": 0.673583984375}, {"start": 1199.05, "end": 1199.17, "word": " أنا", "probability": 0.60107421875}, {"start": 1199.17, "end": 1199.73, "word": " أكبر", "probability": 0.9749348958333334}, {"start": 1199.73, "end": 1200.35, "word": " لازم", "probability": 0.85107421875}, {"start": 1200.35, "end": 1200.61, "word": " أني", "probability": 0.892333984375}, {"start": 1200.61, "end": 1200.71, "word": " أنا", "probability": 0.80517578125}, {"start": 1200.71, "end": 1201.11, "word": " أحصل", "probability": 0.9847005208333334}, {"start": 1201.11, "end": 1201.33, "word": " على", "probability": 0.958984375}, {"start": 1201.33, "end": 1201.69, "word": " دقة", "probability": 0.9384765625}, {"start": 1201.69, "end": 1202.11, "word": " عالية", "probability": 0.9969075520833334}, {"start": 1202.11, "end": 1202.27, "word": " من", "probability": 0.9541015625}, {"start": 1202.27, "end": 1202.71, "word": " خلال", "probability": 0.9970703125}, {"start": 1202.71, "end": 1203.21, "word": " التحكم", "probability": 0.9134114583333334}, {"start": 1203.21, "end": 1203.45, "word": " في", "probability": 0.8857421875}, {"start": 1203.45, "end": 1203.77, "word": " بعض", "probability": 0.9814453125}, {"start": 1203.77, "end": 1203.93, "word": " في", "probability": 0.38134765625}, {"start": 1203.93, "end": 1204.21, "word": " الأمور", "probability": 0.9905598958333334}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 1206.857125, "duration_after_vad": 1156.4143749999994}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/XZSMgPWLPeQ_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/XZSMgPWLPeQ_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..c9e82dc0b8139251d59cd0666b7c7849c8ad13f6
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/XZSMgPWLPeQ_raw.srt
@@ -0,0 +1,1156 @@
+1
+00:00:00,390 --> 00:00:04,130
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية في
+
+2
+00:00:04,130 --> 00:00:07,130
+هذا اللقاء هيكون معملنا بعنوان introduction to the
+
+3
+00:00:07,130 --> 00:00:10,910
+oil emerging compound microscope هنتعرف على ال
+
+4
+00:00:10,910 --> 00:00:14,370
+light compound microscope على المشهر الضوئي المركب
+
+5
+00:00:14,760 --> 00:00:18,700
+استخدامات هذا المجهر و أجزاءه و وظيفة كل جزء
+
+6
+00:00:18,700 --> 00:00:22,740
+بالتفصيل سنتعرف كمان على بعض النقاط المهمة بالنسبة
+
+7
+00:00:22,740 --> 00:00:26,480
+للـ Light Compound Microscope أخر جزئية هنتكلم كيف
+
+8
+00:00:26,480 --> 00:00:31,660
+أنا بدي أحفظ أو أني أحافظ على الـ Microscope و أني
+
+9
+00:00:31,660 --> 00:00:36,760
+أنظفه بعد ما أخلص من شغلفي الـ introduction عرفنا
+
+10
+00:00:36,760 --> 00:00:39,420
+الـ microbiology وقلنا it is the study of
+
+11
+00:00:39,420 --> 00:00:43,500
+microorganism that cannot be seen by next eye من
+
+12
+00:00:43,500 --> 00:00:46,220
+الـ microorganism اللي احنا هيتعامل معها في هذا
+
+13
+00:00:46,220 --> 00:00:50,800
+المثاق اللي هو البكتيريا حجم البكتيريا بيتراوح و
+
+14
+00:00:50,800 --> 00:00:54,000
+معظم حجم البكتيريا بيتراوح ما بين نص لاتنين micro
+
+15
+00:00:54,000 --> 00:00:58,560
+meter micro meter كتير حجم صغير عشان اني انا اقدر
+
+16
+00:00:58,560 --> 00:01:01,920
+اشوفها بحتاج ال microscope فانا بدي اعرف ال
+
+17
+00:01:01,920 --> 00:01:05,800
+microscopeMicroscopy is the technology of making
+
+18
+00:01:05,800 --> 00:01:09,000
+very small things visible to the human eye هو
+
+19
+00:01:09,000 --> 00:01:12,100
+التقنية باستخدامها في تكبير الأشياء الصغيرة التي
+
+20
+00:01:12,100 --> 00:01:16,340
+لا ترى بالعين المجردة used in the diagnosis of
+
+21
+00:01:16,340 --> 00:01:19,640
+many diseases يستخدم الميكروسكوفي تشخيص العديد من
+
+22
+00:01:19,640 --> 00:01:24,120
+الأمراض على سبيل المثال الـ Cancerفي معمل الـ
+
+23
+00:01:24,120 --> 00:01:29,320
+histopathology في معمل الانسجار كنتوا تاخدوا ال
+
+24
+00:01:29,320 --> 00:01:33,220
+biopsy و يتقطعوها و يتمرروها بميراحل و بعدين
+
+25
+00:01:33,220 --> 00:01:36,920
+تصبغوها و بعد كده تشوفوها تحت ال microscope كنتوا
+
+26
+00:01:36,920 --> 00:01:41,260
+تتأكدوا من عدم وجود نمو غير طبيعي للخلايا فبالتالي
+
+27
+00:01:41,260 --> 00:01:45,100
+انكوا تقدروا تشخصوا هل عند المريض cancer أو لأ
+
+28
+00:01:45,100 --> 00:01:51,630
+used for soil, insect and rock تستخدم في التربةوفي
+
+29
+00:01:51,630 --> 00:01:55,210
+الـ insect للحشرات لو أنا بدي أشوف حشر أشوف
+
+30
+00:01:55,210 --> 00:01:59,530
+التفاصيل طبعتها بشوفها تحت ال microscope بالنسبة
+
+31
+00:01:59,530 --> 00:02:04,150
+للسخور المجاهر بنستخدمها في تحليل التركيب المعدني
+
+32
+00:02:04,150 --> 00:02:08,190
+لبعض السخور used in pathological laboratories and
+
+33
+00:02:08,190 --> 00:02:12,010
+medical بنستخدم ال microscope في المختبرات طبعا
+
+34
+00:02:12,010 --> 00:02:16,540
+أكيد مافي أي مختبر مافي موجود microscopeلو انا
+
+35
+00:02:16,540 --> 00:02:20,880
+مثلا بدي اعمل فيلم للدم بصفغه بصبخات خاصة وبشوفه
+
+36
+00:02:20,880 --> 00:02:24,960
+تحت ال microscope من خلاله هيتم تشخيص العديد من
+
+37
+00:02:24,960 --> 00:02:28,560
+أمراض الدم المختلفة من الانيميا واللوكيميا
+
+38
+00:02:28,560 --> 00:02:32,520
+industrial microscope used for metals and various
+
+39
+00:02:32,520 --> 00:02:37,080
+kinds of measurements نستخدم ال microscope للمعادن
+
+40
+00:02:37,480 --> 00:02:41,100
+educational microscope used for research أكيد لأ
+
+41
+00:02:41,100 --> 00:02:46,860
+هدف تعليمي وأيضا في مجار الأبحاث وفي رؤية تركيب
+
+42
+00:02:46,860 --> 00:02:51,540
+الخلايا النباتية والحيوانية وغيرها من التطبيقات
+
+43
+00:02:51,540 --> 00:02:56,220
+bacteria is one of the small microscope that
+
+44
+00:02:56,220 --> 00:02:58,540
+cannot be seen with the naked eye اتفقنا أن
+
+45
+00:02:58,540 --> 00:03:01,260
+البكتيريا من الكائنات الدقيقة التي لا ترى بالعين
+
+46
+00:03:01,260 --> 00:03:01,980
+المجردة
+
+47
+00:03:04,330 --> 00:03:09,850
+معظم حجم البكتيريا يتراوح بين نصف لإتنين ميكرو متر
+
+48
+00:03:09,850 --> 00:03:14,110
+وهذا اتفقنا عليه في أول المعمل لذلك يحتاج الناس
+
+49
+00:03:14,110 --> 00:03:17,530
+لمجموعة البكتيريا للوقت السفيري باستخدام ميكروسكوب
+
+50
+00:03:17,530 --> 00:03:22,050
+لرؤيه، عشان هيك أنا بحتاج الميكروسكوب حتى أني أنا
+
+51
+00:03:22,050 --> 00:03:27,170
+أكبرها عشان أقدر أشوفها لكن أكيد احنا قلنا إن معظم
+
+52
+00:03:27,170 --> 00:03:30,570
+حجم البكتيريا بيتراوح ما بين نصف لإتنين ميكرو متر
+
+53
+00:03:31,070 --> 00:03:34,630
+أكيد لكل قاعدة شواز في علم الـ Microbiology مافي
+
+54
+00:03:34,630 --> 00:03:39,050
+اشي Absolute مافي اشي مطلق كل اشي له استثناء من
+
+55
+00:03:39,050 --> 00:03:42,410
+هذه الاستثناء ان انا في عيني Giant Bacteria اسمها
+
+56
+00:03:42,410 --> 00:03:47,750
+Thiomargarita Namibiansi هذه البكتيريا تعتبر Giant
+
+57
+00:03:47,750 --> 00:03:51,970
+Bacteria بقدر اشوفها في عيني المجردة بيتراوح حجم
+
+58
+00:03:51,970 --> 00:03:55,910
+هذه البكتيريا ما بين 1 من 10 ل 3 من 10 ملي متر لو
+
+59
+00:03:55,910 --> 00:04:00,770
+انا بدي حوّلها ل micro meterتروح ما بين 100 لـ 300
+
+60
+00:04:00,770 --> 00:04:04,630
+ميكرو متر في الـ Diameter وبعض الأحيان ممكن توصل
+
+61
+00:04:04,630 --> 00:04:10,870
+لـ 75 من 100 ميلي متر أي 750 ميكرو متر يبقى جملة
+
+62
+00:04:10,870 --> 00:04:14,310
+كل البكتيريا لتراب العين المجرة ده غلط لأنه أنا
+
+63
+00:04:14,310 --> 00:04:17,430
+فيها عند ستنقة فيها عندي تيو مارجاريتا نامي
+
+64
+00:04:17,430 --> 00:04:20,730
+بيانسيا هنالك مختلفة نوعات من الميكروسكوب التي
+
+65
+00:04:20,730 --> 00:04:23,810
+تستخدمها في حياة الميكروبيا وقلنا إنه احنا بنعرف
+
+66
+00:04:23,810 --> 00:04:27,250
+أنواع مختلفة من الميكروسكوب على سبيل المثال أنا
+
+67
+00:04:27,250 --> 00:04:30,210
+فيه عنديالـ Light Microscope يعني دي الـ Light
+
+68
+00:04:30,210 --> 00:04:32,830
+Compound Microscope يعني دي الـ Fluorescent الـ
+
+69
+00:04:32,830 --> 00:04:36,090
+Electron Microscope كل هذه الميكروسكوبات أنا
+
+70
+00:04:36,090 --> 00:04:42,110
+بستخدمها في علم الـ Microbiology لكن in this lab
+
+71
+00:04:42,110 --> 00:04:44,890
+you will become familiar with the use of Light
+
+72
+00:04:44,890 --> 00:04:48,390
+Compound Microscope فهذا المعمل هنتعرف على الـ
+
+73
+00:04:48,390 --> 00:04:51,590
+Light Compound Microscope الـ Light Compound
+
+74
+00:04:51,590 --> 00:04:54,270
+Microscope ليش أنا سميته بهذا الإسم الـ Light لأن
+
+75
+00:04:54,270 --> 00:04:58,520
+الضوء بيمر خلال الجسم المرات كبيرهcompound لأنه
+
+76
+00:04:58,520 --> 00:05:02,220
+بيتركب من عدستان عدسة عينية الـ ocular lens وعدسة
+
+77
+00:05:02,220 --> 00:05:06,020
+شيئية الـ objective lens and will compare the
+
+78
+00:05:06,020 --> 00:05:09,800
+relative size and shape of various microorganisms
+
+79
+00:05:09,800 --> 00:05:13,740
+من خلاله أنا بتعرف على الحجم النسبي للبكتيريا
+
+80
+00:05:13,740 --> 00:05:20,340
+وأيضا أشكال الكائنات الدقيقة المختلفة microscope
+
+81
+00:05:20,340 --> 00:05:23,680
+consists of four major parts بقولك المايكروسكوب
+
+82
+00:05:23,680 --> 00:05:28,470
+بتكون من أربع مكونات أساسيةأول مكوّن عندنا اللي هو
+
+83
+00:05:28,470 --> 00:05:31,950
+الـ Framework الـ Framework اللي هو إطار العمل
+
+84
+00:05:31,950 --> 00:05:36,010
+إطار العمل بتكون من تلت أجزاء Arm, Stache, Pulse
+
+85
+00:05:36,010 --> 00:05:40,350
+هنتكلم عنها بالتفصيل عندي تاني جزء من أزاق الـ
+
+86
+00:05:40,350 --> 00:05:44,310
+Microscope المهمة الـ Adjustment System نظام
+
+87
+00:05:44,310 --> 00:05:48,090
+الضابط نظام الضابط بتكون من الـ Fine Adjustment و
+
+88
+00:05:48,090 --> 00:05:51,230
+الـ Coarse Adjustment اللي هو الضابط الصغير و
+
+89
+00:05:51,230 --> 00:05:54,860
+الضابط الكبيرالتالت جزء اللي هو الـ Magnification
+
+90
+00:05:54,860 --> 00:05:57,840
+التكبير المسؤول عن التكبير في الـ Microscope اللي
+
+91
+00:05:57,840 --> 00:06:02,520
+هم العدسات الـ Ocular Lens العدسة العينية و الـ
+
+92
+00:06:02,520 --> 00:06:06,500
+Objective Lens العدسات الشيئية آخر جزء اللي هو الـ
+
+93
+00:06:06,500 --> 00:06:09,880
+Lighting System اللي هو نظام الإضاءة فيه عند الـ
+
+94
+00:06:09,880 --> 00:06:14,000
+Condenser اللي هو المكثف اللي بيجمع الضوء و إداف
+
+95
+00:06:14,000 --> 00:06:17,680
+راجم اللي هو بتحكم في كمية الضوء المار عبر العين
+
+96
+00:06:24,230 --> 00:06:26,990
+في هاي الـ slide هنتكلم بالتفصيل عن أجزاء
+
+97
+00:06:26,990 --> 00:06:31,830
+الميكروسكوب ووظيفة كل جزء في عندي أول جزء الـ
+
+98
+00:06:31,830 --> 00:06:36,030
+ocular lens العدسات العينية في عندي two ocular
+
+99
+00:06:36,030 --> 00:06:40,190
+lens فدول العدسات العينية شو وظيفتهم؟ remagnify
+
+100
+00:06:40,190 --> 00:06:44,670
+the image بيعمل على إعادة تكبير الصورة طيب مين
+
+101
+00:06:44,670 --> 00:06:49,810
+اللي بيعمل magnify؟ مين المسؤول الأساسي والأول عن
+
+102
+00:06:49,810 --> 00:06:54,010
+تكبير الصورة؟ نكمل الجملةformed by the objective
+
+103
+00:06:54,010 --> 00:06:57,970
+lens اللي بيتم تكبيرها بواسطة الـ objective lens
+
+104
+00:06:57,970 --> 00:07:02,630
+يبقى الـ objective lens اللي هي العدسات الشيئية هي
+
+105
+00:07:02,630 --> 00:07:06,150
+ال primary lens that magnify the specimen هي
+
+106
+00:07:06,150 --> 00:07:09,990
+العدسة الأولية اللي بتكبرلي الصورة طبعا لأن هي
+
+107
+00:07:09,990 --> 00:07:13,910
+قريبة من العينة فأكيد هتكون هي العدسة الأولية اللي
+
+108
+00:07:13,910 --> 00:07:19,180
+بتكبر الصورةremagnify الـ ocular lens بتكبّلي
+
+109
+00:07:19,180 --> 00:07:24,340
+بعدها بتوصل للصورة للعدسة العينية و بتكبّلي إياها
+
+110
+00:07:24,340 --> 00:07:29,040
+حتى إن أنا أحصل بالنهاية على total magnification
+
+111
+00:07:29,040 --> 00:07:31,800
+إيش يعني total magnification؟ وحصل ضرب ال
+
+112
+00:07:31,800 --> 00:07:35,020
+objective magnification قوة تكبير العدسة الشيئية
+
+113
+00:07:35,020 --> 00:07:38,840
+اللي أنا استخدمتها مع قوة تكبير العدسة العينية
+
+114
+00:07:38,840 --> 00:07:42,380
+الـocular magnification اللي هي العشرة X قوة تكبير
+
+115
+00:07:42,380 --> 00:07:46,860
+العدسة العينية عشرة X لكنقوة تكبير العدسة الشيئية
+
+116
+00:07:46,860 --> 00:07:51,340
+في عيني انا اربع عدسات اربع عشرة اربعين مية فهو
+
+117
+00:07:51,340 --> 00:07:55,080
+استخدمت بدربها في قوة تكبير العدسة العينية العشرة
+
+118
+00:07:55,080 --> 00:07:59,940
+X حتى احصل على ال total magnification بالنسبة
+
+119
+00:07:59,940 --> 00:08:05,140
+للعدسات الشيئية العدسات الشيئية مربوطين باشي اسمه
+
+120
+00:08:05,140 --> 00:08:11,910
+nosepiece هذاهو الـ Nosepiece قُرص دائري أو جزء
+
+121
+00:08:11,910 --> 00:08:15,970
+دائري بيحمل العدسات الشيئية بنتحكم فيه من خلال
+
+122
+00:08:15,970 --> 00:08:20,170
+التدوير وبالتالي بيؤدي لغيير قوة التكبير اللي أنا
+
+123
+00:08:20,170 --> 00:08:23,750
+بدي إياها أربعة أو عشرة أو أربعين أو مية حسب أنا
+
+124
+00:08:23,750 --> 00:08:30,810
+شو ما بدي بالنسبة للـ Body نيجي للـ Body الـ Body
+
+125
+00:08:30,810 --> 00:08:34,310
+هو إيه اسم تاني اللي هو الـ Optical Tube الأنبوب
+
+126
+00:08:34,310 --> 00:08:38,250
+البصريtransmit the image from the objective lens
+
+127
+00:08:38,250 --> 00:08:42,670
+to the ocular lens using prism بقولك بينقل الصورة
+
+128
+00:08:42,670 --> 00:08:46,570
+من ال objective lens لال ocular lens ننتبه أنه مش
+
+129
+00:08:46,570 --> 00:08:49,950
+العكس لأنه احنا قلنا أن ال objective lens هي
+
+130
+00:08:49,950 --> 00:08:54,410
+المسئولة عن التكبير بشكل أولي لأنه هي المسئولة
+
+131
+00:08:54,410 --> 00:08:56,910
+الأولى عن التكبير فبالتالي هو بينقل من ال
+
+132
+00:08:56,910 --> 00:09:02,240
+objective لالـ Ocular Lens الـ Arm اللي هو الذراع،
+
+133
+00:09:02,240 --> 00:09:05,260
+الذراع أنا بستخدمه لحمل الـ Microscope و نقله من
+
+134
+00:09:05,260 --> 00:09:09,960
+مكان إلى آخر بالنسبة للـ Stage، الـ Stage هي عبارة
+
+135
+00:09:09,960 --> 00:09:14,000
+عن سيما أحنا شايفين سطح مستوي يوجد تحت العدسات
+
+136
+00:09:14,000 --> 00:09:17,920
+الشيئية مخصص لوضع الشريحة عليه hold the microscope
+
+137
+00:09:17,920 --> 00:09:22,100
+slide in position مخصص لوضع ال slide أو الشريحة
+
+138
+00:09:22,100 --> 00:09:27,210
+عليه فيندي أنا ملقتان clipsبساعد في تثبيت الشريحة
+
+139
+00:09:27,210 --> 00:09:31,190
+على الاستاج وضمان عدم تحركها أثناء شغله في موجود
+
+140
+00:09:31,190 --> 00:09:34,710
+تحت اللي هو الاستاج شيء اسمه الـ Condenser اللي هو
+
+141
+00:09:34,710 --> 00:09:38,890
+المكثف مش وظيفته focus light through the specimen
+
+142
+00:09:38,890 --> 00:09:44,450
+بيقولك بيجمع أو بيركز الشقة الضوء المتجهة نحو
+
+143
+00:09:44,450 --> 00:09:49,830
+الشريحة وبعدين هتمار عبر العدسات الشيئية طبعا
+
+144
+00:09:51,530 --> 00:09:54,230
+الكندنسر فيه تحته «Diaphragm» مايعني الـ
+
+145
+00:09:54,230 --> 00:09:57,270
+«Diaphragm» مسئول عن «control of the amount of
+
+146
+00:09:57,270 --> 00:10:01,290
+light entering the condenser» بتحكم بكمية الضوء
+
+147
+00:10:01,290 --> 00:10:05,930
+الواصلة للكندنسر وبعدين هتوصل للعينة ثم العدسات
+
+148
+00:10:05,930 --> 00:10:10,950
+الشيئية في عندي أكيد مصدر للضوء أخر الشيء في عندي
+
+149
+00:10:10,950 --> 00:10:16,210
+ضابطين على جانبي المشهر ضابط كبير «course focusing
+
+150
+00:10:16,210 --> 00:10:20,890
+knob» وضابط صغير «fine focusing knob»شو وظيفة الـ
+
+151
+00:10:20,890 --> 00:10:24,610
+Course Focusing Knob؟ وظيفته «Move the stage up
+
+152
+00:10:24,610 --> 00:10:27,210
+and down to focus the image» بقولك بحرك الـ
+
+153
+00:10:27,210 --> 00:10:31,330
+«stage» لفقه و لتحت حتى أنه يثبت الصورة مش يعني
+
+154
+00:10:31,330 --> 00:10:35,410
+focus the image نفس معناه أنه أنا عشان أقدر أجيب
+
+155
+00:10:35,410 --> 00:10:38,890
+ال field عشان أقدر أجيب ال field بحرك ال «stage»
+
+156
+00:10:38,890 --> 00:10:42,610
+لأعلى أو لأسفل حتى أقدر أجيبه في الصورة اللي أنا
+
+157
+00:10:42,610 --> 00:10:46,680
+بديها هيك أنا معناه اقدرت أجيب ال fieldالـ «Find
+
+158
+00:10:46,680 --> 00:10:50,240
+Focusing Knob» هو الضابط الصغير الوظيفته اللي هو
+
+159
+00:10:50,240 --> 00:10:54,000
+الـ «Clarity» الوضوح حتى إنه يزيد وضوح الصورة أنا
+
+160
+00:10:54,000 --> 00:10:58,480
+بستخدم بعد ما أجيب الفيل تمام جبت الفيل لو انتقلت
+
+161
+00:10:58,480 --> 00:11:03,180
+لعدسة «High Power» لقوة تكبير أعلى بلاقي الصورة
+
+162
+00:11:03,180 --> 00:11:07,020
+مغبشة فأنا بروح بلعب بالـ «Find» عشان إنه تزيد
+
+163
+00:11:07,020 --> 00:11:10,420
+وضوح الصورة تزيد الـ «Clarity» الـ «Base» هي
+
+164
+00:11:10,420 --> 00:11:15,040
+الركيزة الأساسية للمشهر واللي هي بتحمل الـ
+
+165
+00:11:15,040 --> 00:11:19,720
+«Microscope»هذا بالنسبة لأجزاء الـ «مايكروسكوب»
+
+166
+00:11:19,720 --> 00:11:23,960
+تعرفنا على وظيفة
+
+167
+00:11:23,960 --> 00:11:27,800
+كل جزء لكن هنتكلم بالتفصيل عن كل جزء في الـ
+
+168
+00:11:27,800 --> 00:11:29,240
+«سلايدات» اللي بعيدة
+
+169
+00:11:38,830 --> 00:11:42,710
+تكلمنا إن الميكروسكوب بتكون من أربع أزاق من ضمن
+
+170
+00:11:42,710 --> 00:11:45,370
+هذه الأجزاء أول شيء اللي هو الـ Framework الـ
+
+171
+00:11:45,370 --> 00:11:48,450
+Framework بتكون من تلت أجزاء الـ Base اللي هي
+
+172
+00:11:48,450 --> 00:11:51,850
+القاعدة و الـ Arm اللي هو الذراع من خلال اللي قلنا
+
+173
+00:11:51,850 --> 00:11:55,590
+بحمل الميكروسكوب وبنقله من مكان لآخر و الـ
+
+174
+00:11:55,590 --> 00:11:58,730
+Mechanical Stage طب أنا لو بدي أعرف شو هي الـ
+
+175
+00:11:58,730 --> 00:12:02,550
+Stage Stage is the location of the specimen to be
+
+176
+00:12:02,550 --> 00:12:07,930
+viewed أقولك هو الموقع اللي أنا بحط في الشريحة أو
+
+177
+00:12:07,930 --> 00:12:11,630
+العينةEclipse utilized in holding the specimen in
+
+178
+00:12:11,630 --> 00:12:14,330
+place هاي طبعاً هي كلها الـ stage في عندي أنا
+
+179
+00:12:14,330 --> 00:12:17,870
+clips هاي هي الـ clips هي الملاقت اللي بتستخدم
+
+180
+00:12:17,870 --> 00:12:21,290
+لتثبيت العين على الـ stage و ضمان عدم تحركها
+
+181
+00:12:21,290 --> 00:12:25,130
+mechanical stage انه هي ممكن انا احركها كيف انا
+
+182
+00:12:25,130 --> 00:12:29,390
+ممكن احركها باستخدام الـ stage knob control في
+
+183
+00:12:29,390 --> 00:12:32,730
+عندي top knob و في عندي bottom knob هذا هو ال top
+
+184
+00:12:32,730 --> 00:12:36,590
+knob وهذا ال bottom knobهدولا الـ Stage knob
+
+185
+00:12:36,590 --> 00:12:41,310
+control أنا بحرك فيهم الـ Stage لليمين و لا اليسار
+
+186
+00:12:41,310 --> 00:12:44,490
+و لا الأمام و لا الخلف طيب متى أنا بحتاج هذا
+
+187
+00:12:44,490 --> 00:12:48,810
+الاشياء على سبيل المثال أنا في عندي slide و بدي
+
+188
+00:12:48,810 --> 00:12:52,530
+أشوف كل هذه الشريحة بدي أمر على كل هذه الشريحة
+
+189
+00:12:52,530 --> 00:12:58,290
+فعشان أنا ما أضيع ولا field بروح بمشي زيجزاج بمشي
+
+190
+00:12:58,290 --> 00:13:02,150
+بهذه الطريقة حتى إن أنا أضمن إن أنا مريت على كل
+
+191
+00:13:02,150 --> 00:13:03,430
+الشريحة
+
+192
+00:13:05,580 --> 00:13:09,580
+طبعا فانا هان هستخدم ال stage knob control حتى
+
+193
+00:13:09,580 --> 00:13:13,160
+انها تساعدني في الحركة فعشان انا اول شي هبدأ
+
+194
+00:13:13,160 --> 00:13:18,640
+بالحركة للامام فعشان انا اتحرك للامام هستخدم ال
+
+195
+00:13:18,640 --> 00:13:22,060
+top knob is to move the forward and backward
+
+196
+00:13:22,060 --> 00:13:25,720
+هيحركلي ال stage للامام و للخلف انا بدي اتحرك
+
+197
+00:13:25,720 --> 00:13:28,900
+للامام هستخدم ال top knob فبدي ارجع في field لأ
+
+198
+00:13:28,900 --> 00:13:31,400
+بدي ارجع اشوفه بقى استخدم برضه ال top knob
+
+199
+00:13:31,400 --> 00:13:37,280
+بحركليها للامام و للخلفطيب بالنسبة للـ Bottom Knob
+
+200
+00:13:37,280 --> 00:13:42,800
+أنا خلصت كل هذه المنطقة بدي أروح هاجي أتحرك لليمين
+
+201
+00:13:42,800 --> 00:13:46,540
+فبستخدم الـ Bottom Knob is to move the stage right
+
+202
+00:13:46,540 --> 00:13:50,400
+and left يبقى أعرفنا ليش أنا بستخدم الـ Top Knob و
+
+203
+00:13:50,400 --> 00:13:53,720
+الـ Bottom Knob الـ Top حتى أني أنا أحرك للأمام و
+
+204
+00:13:53,720 --> 00:13:57,720
+للخلف و الـ Bottom حتى أني أحرك للـSlide لليمين و
+
+205
+00:13:57,720 --> 00:13:58,680
+للشمال
+
+206
+00:14:05,000 --> 00:14:08,180
+نجي لخلصنا من أول مكون من مكونات الـ Microscope
+
+207
+00:14:08,180 --> 00:14:11,920
+اللي هو الـ Adjustment System الـ Framework نجي
+
+208
+00:14:11,920 --> 00:14:15,120
+لتاني مكون اللي هو الـ Adjustment System الـ
+
+209
+00:14:15,120 --> 00:14:18,640
+Adjustment System هو نظام الضبطبتكون من الـ
+
+210
+00:14:18,640 --> 00:14:22,720
+Optical Tube الـ Optical Tube اللي هو اللي عرفناه
+
+211
+00:14:22,720 --> 00:14:26,080
+في الأول slide في المعمل كان الـ Body اللي هو
+
+212
+00:14:26,080 --> 00:14:32,340
+بينقل الصورة من الـ Objective Lens للـ Ocular Lens
+
+213
+00:14:32,340 --> 00:14:35,540
+يعني بيروبط ما بين العدسة العينية وما بين العدسة
+
+214
+00:14:35,540 --> 00:14:39,360
+الشيئية في عندي الcourse adjustment الضابط الكبير
+
+215
+00:14:39,360 --> 00:14:42,720
+و ال fine adjustment اللي هو الضابط الصغير هنتعرف
+
+216
+00:14:42,720 --> 00:14:46,050
+عليهم في هاي ال slideالـ Course Adjustment
+
+217
+00:14:46,050 --> 00:15:13,790
+Knob�
+
+218
+00:15:14,270 --> 00:15:18,630
+bringing the objective هي إن حتى إن أنا أجيب ال
+
+219
+00:15:18,630 --> 00:15:21,990
+field الضابط الكبير وظيفته إن أنا أقدر أجيب ال
+
+220
+00:15:21,990 --> 00:15:24,970
+field ال field هو الصورة اللي أنا بدي أشوف هيبقى
+
+221
+00:15:24,970 --> 00:15:28,530
+bringing the image أو focus the image هيوظيفة ال
+
+222
+00:15:28,530 --> 00:15:31,690
+course adjustment knob بالنسبة لأ ال fine
+
+223
+00:15:31,690 --> 00:15:35,690
+adjustment knob is used for improving the clarity
+
+224
+00:15:35,690 --> 00:15:39,610
+حتى إنه يزيد وضوح الصورة especially when viewing
+
+225
+00:15:39,610 --> 00:15:43,690
+under high powerخصوصًا لما أنا أستخدم الـHigh
+
+226
+00:15:43,690 --> 00:15:47,970
+Power مش قصده بيه هذه الجملة خلال الشغل العملي لما
+
+227
+00:15:47,970 --> 00:15:51,770
+أنا أجيب الـField على 4X و بعدين أروح أحول على 40X
+
+228
+00:15:51,770 --> 00:15:55,110
+أو على 100 اللي هي الـHigh Power بلاقي الصورة
+
+229
+00:15:55,110 --> 00:15:59,950
+مغبشة فعشان أنا أزيد وضوح الصورة يتكون improving
+
+230
+00:15:59,950 --> 00:16:02,850
+the clarity of the image بروح بحرك الـFine
+
+231
+00:16:02,850 --> 00:16:06,930
+Adjustment شوية فبالتالي هيك بتكون الصورة واضحة
+
+232
+00:16:06,930 --> 00:16:09,550
+وفي بعض الأمور التانية اللي ممكن كمان إن أنا أتحكم
+
+233
+00:16:09,550 --> 00:16:14,770
+فيها نتكلم عنهافي الـ «سلايدات» اللي جاية فأنا هان
+
+234
+00:16:14,770 --> 00:16:17,390
+بأستخدم فقط الـ «fine adjustment» جبت الـ «field»
+
+235
+00:16:17,390 --> 00:16:21,110
+ممنوع أستخدم الـ «course adjustment» خلاص فقط بحرك
+
+236
+00:16:21,110 --> 00:16:25,410
+الـ «fine adjustment» عشان تزيد وضوح الصورة ممنوع
+
+237
+00:16:25,410 --> 00:16:28,750
+أمسك الـ «course adjustment» هذا بالنسبة لـ
+
+238
+00:16:28,750 --> 00:16:32,770
+«course» والـ «fine adjustment knob» هاي مكونات
+
+239
+00:16:32,770 --> 00:16:37,290
+الـ «adjustment system» نيجي لآخر مكون اللي هو الـ
+
+240
+00:16:37,290 --> 00:16:39,930
+«interbiolary adjustment»
+
+241
+00:16:41,930 --> 00:16:45,210
+بشهدها الـ Inter-bipolary adjustment ‏to control
+
+242
+00:16:45,210 --> 00:16:48,230
+the distance ‏between the ocular lens ‏to adopt
+
+243
+00:16:48,230 --> 00:16:52,910
+the distance between viewer eye ‏قولنا إن المسافة
+
+244
+00:16:52,910 --> 00:16:58,750
+بين عينان أي شخص بتختلف ‏فبالتالي عشان أتحكم في
+
+245
+00:16:58,750 --> 00:17:03,170
+المسافة ‏ما بين العدسات العينية ‏حتى إنها تكون
+
+246
+00:17:03,170 --> 00:17:07,610
+مناسبة ‏لمسافة ما بين عينان الرأي ‏هذه أنا بستخدم
+
+247
+00:17:07,610 --> 00:17:12,950
+الـ Inter-bipolary adjustmentالـ Eye-Base Lens هي
+
+248
+00:17:12,950 --> 00:17:17,430
+نفسها الـ Ocular Lens ممكن هو الـ Spread apart
+
+249
+00:17:17,430 --> 00:17:22,290
+ممكن أبعدهم عن بعض أو Get closer together to fit
+
+250
+00:17:22,290 --> 00:17:28,130
+each individual أو ممكن أقربهم حسب شخص بيرتاح حسب
+
+251
+00:17:28,130 --> 00:17:32,690
+ما بيرتاح ممكن أن أنا أبعدهم أو أقربهم هذا بالنسبة
+
+252
+00:17:32,690 --> 00:17:34,410
+للـ Adjustment
+
+253
+00:17:42,630 --> 00:17:45,930
+ثالث مكوّن من مكونات الميكروسكوب الـ Magnification
+
+254
+00:17:45,930 --> 00:17:48,590
+قلنا المسؤول عن الـ Magnification العدسات الـ
+
+255
+00:17:48,590 --> 00:17:51,530
+Ocular Lens العدسات العينية الـ IBIS اللي هي اسم
+
+256
+00:17:51,530 --> 00:17:54,910
+تاني و الـ Objective Lens اللي هي العدسات الشيئية
+
+257
+00:17:54,910 --> 00:17:59,510
+الـ Ocular Lens or الـ IBIS is used for viewing
+
+258
+00:17:59,510 --> 00:18:04,510
+العدسات العينية تستخدم للرؤية Revolving Nose Views
+
+259
+00:18:04,510 --> 00:18:07,810
+contain Objective Lens that are used to magnify
+
+260
+00:18:07,810 --> 00:18:12,270
+قلنا إن في أندي أنا هان إيش اسمهNosepiece هذا هو
+
+261
+00:18:12,270 --> 00:18:16,770
+الـ Nosepiece قرص دائري من خلاله أنا بدور العدسات
+
+262
+00:18:16,770 --> 00:18:20,770
+الشيئية حسب أنا شو بدي بحرك و بختار العدسة الشيئية
+
+263
+00:18:20,770 --> 00:18:25,210
+اللي أنا بدي استخدمها بقولك هذا اسمه في العربية
+
+264
+00:18:25,210 --> 00:18:28,650
+فيه اسم اللي هو الطاحونة بيحتوى بيحمل العدسات
+
+265
+00:18:28,650 --> 00:18:33,220
+الشيئية شو وظيفة العدسات؟تستخدم لـ Magnify لتكبير
+
+266
+00:18:33,220 --> 00:18:36,880
+الصورة بالاتحاد مع الـ Ocular Lens واتفقنا إنه
+
+267
+00:18:36,880 --> 00:18:39,560
+بالنهاية أنا بحصل على Total Magnification من حصل
+
+268
+00:18:39,560 --> 00:18:41,980
+ضرب الـ Magnification للعدسات العينية في الـ
+
+269
+00:18:41,980 --> 00:18:45,720
+Magnification للعدسات الشائعية The objective of
+
+270
+00:18:45,720 --> 00:18:48,760
+microscope is not just to increase magnification
+
+271
+00:18:48,760 --> 00:18:53,440
+but to do so while attaining sufficient resolution
+
+272
+00:18:54,450 --> 00:18:57,230
+في هذه الجملة في أول معمل الـ Microscope قلنا إن
+
+273
+00:18:57,230 --> 00:19:01,150
+الـ Microscope هو تقنية بيجعل الأشياء الصغيرة يمكن
+
+274
+00:19:01,150 --> 00:19:05,070
+رؤيتها بالعين المجردة من خلال تكبيرها بالجملة هذه
+
+275
+00:19:05,070 --> 00:19:08,690
+بقول إن أنا مش هدفي من الـ Microscope فقط إن أنا
+
+276
+00:19:08,690 --> 00:19:12,750
+أكبر الأشياء كمان لازم أحصل على دقة عالية للصورة
+
+277
+00:19:12,750 --> 00:19:16,830
+تكون واضحة على سبيل المثال لو وقت كتير بنزل صورة
+
+278
+00:19:16,830 --> 00:19:20,090
+على الجوال بروح أنا بعمل zoom بكبر عشان أشوف بعض
+
+279
+00:19:20,090 --> 00:19:25,980
+التفاصيل ما بقدر أشوفها بشكل واضحفبالتالي هان ممكن
+
+280
+00:19:25,980 --> 00:19:30,640
+تكون الصورة مكبرة ولكن مش واضحة نطبق الموضوع على
+
+281
+00:19:30,640 --> 00:19:33,980
+الـ Microscope لما أنت تجيبي الـ Field على 10X و
+
+282
+00:19:33,980 --> 00:19:38,180
+تروحي على 40X الـ High Power هتلاحظي أن الصورة
+
+283
+00:19:38,180 --> 00:19:41,780
+مغبشة و مش واضحة عشان تحصلي على Resolution عالي
+
+284
+00:19:41,780 --> 00:19:45,580
+على دقة عالية بتروحي بتحركي الـ Point Adjustment
+
+285
+00:19:45,580 --> 00:19:50,710
+اللي هو الضابط الصغير حتى تحصلي على دقة عاليةوكمان
+
+286
+00:19:50,710 --> 00:19:54,790
+ممكن أنه نتحكم في أمور تانية هنتكلم عنها في الـ
+
+287
+00:19:54,790 --> 00:19:58,770
+Slides اللي جاي يبقى مش بس الهدف من الـ Microscope
+
+288
+00:19:58,770 --> 00:20:02,270
+أني أنا أكبر لازم أني أنا أحصل على دقة عالية من
+
+289
+00:20:02,270 --> 00:20:04,210
+خلال التحكم في بعض في الأمور
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/XkWBgngbjy4.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/XkWBgngbjy4.srt
new file mode 100644
index 0000000000000000000000000000000000000000..f6d3b12153b965ec1701303008adb2e17cfb088b
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/XkWBgngbjy4.srt
@@ -0,0 +1,1288 @@
+1
+00:00:00,690 --> 00:00:03,830
+تاني نوع من أنواع الـ special stain، الـ capsular
+
+2
+00:00:03,830 --> 00:00:06,490
+stain، قلنا إنه الـ capsule أحد الـ special
+
+3
+00:00:06,490 --> 00:00:09,770
+components اللي عدم وجودها لا يؤثر على الخلية
+
+4
+00:00:09,770 --> 00:00:13,190
+البكتيرية. يعني في عندي بكتيريا capsulated وفي
+
+5
+00:00:13,190 --> 00:00:17,470
+بكتيريا non-capsulated. capsule are gelatinous
+
+6
+00:00:17,470 --> 00:00:22,010
+outer layer structure. هو طبقة خارجية secreted by
+
+7
+00:00:22,010 --> 00:00:25,730
+bacterial cells that lay outside of the organism
+
+8
+00:00:25,730 --> 00:00:30,060
+cell wall. بكون خارج الخلية البكتيرية، and these are
+
+9
+00:00:30,060 --> 00:00:32,900
+in direct contact with the environment. فبالتالي
+
+10
+00:00:32,900 --> 00:00:36,680
+هيكون ملامس للبيئة الخارجية. الـ capsule بيتم
+
+11
+00:00:36,680 --> 00:00:40,540
+تصنيعه في الـ cytoplasm و يُسرّح إلى الخارج
+
+12
+00:00:40,540 --> 00:00:45,240
+of the cell. وبيطلع خارج الخلية البكتيرية، where it
+
+13
+00:00:45,240 --> 00:00:49,340
+surrounds the bacteria. وبحيط في هذه الخلية. Many
+
+14
+00:00:49,340 --> 00:00:53,700
+perhaps most bacteria produce capsules under the
+
+15
+00:00:53,700 --> 00:00:56,860
+right conditions. بيقول لي معظم البكتيريا بتكون
+
+16
+00:00:56,860 --> 00:01:00,320
+الكبسول في الـ right conditions. إيش يعني في الـ right
+
+17
+00:01:00,320 --> 00:01:03,120
+conditions؟ يعني لو كانت الظروف مناسبة أو كان في
+
+18
+00:01:03,120 --> 00:01:08,860
+عندي مغذيات. المغذيات الزائدة تتخزن
+
+19
+00:01:08,860 --> 00:01:13,040
+في الكبسول. من وظائف الكبسول إنه يعتبر مخزن للمواد
+
+20
+00:01:13,040 --> 00:01:16,100
+الغذائية في حال كان في عندي فائض.
+
+21
+00:01:19,770 --> 00:01:25,650
+Capsules are fragile. بقول لي إن الكبسول هش، and can
+
+22
+00:01:25,650 --> 00:01:30,370
+be damaged, dissociated, or destroyed
+
+23
+00:01:30,370 --> 00:01:34,550
+by heat. بقول لي إن الكبسول if fragile، فبالتالي
+
+24
+00:01:34,550 --> 00:01:40,310
+هيتقلص، وهَيصير جاف، وهيتشوّه، ويتدمر بواسطة
+
+25
+00:01:40,310 --> 00:01:44,950
+الحرارة. من خطوات الـstaining قلنا إن في عندي أول شيء smear
+
+26
+00:01:44,950 --> 00:01:49,540
+preparation، air drying، heat fixation. بما أن
+
+27
+00:01:49,540 --> 00:01:53,660
+الكابسول fragile وبيتكسر بواسطة الحرارة، يبقى خطوة
+
+28
+00:01:53,660 --> 00:01:58,360
+الـ heat fixation في الـ capsular stain مش هنعملها، لأنه
+
+29
+00:01:58,360 --> 00:02:02,400
+هيدمر الكابسول من خلال هذه الخطوة لو أنا عملتها.
+
+30
+00:02:02,400 --> 00:02:08,060
+فبالتالي هيعطيني false result. Most capsules are
+
+31
+00:02:08,060 --> 00:02:13,440
+composed of polysaccharide. بقول لي معظم البكتيريا
+
+32
+00:02:13,440 --> 00:02:16,880
+اللي بتكون كابسول، هي كابسول عبارة عن
+
+33
+00:02:16,880 --> 00:02:21,370
+polysaccharide، but some are composed of polypeptide.
+
+34
+00:02:21,370 --> 00:02:25,750
+لكن في بعض البكتيريا، الكابسول تبعها polypeptide، زي
+
+35
+00:02:25,750 --> 00:02:31,170
+البَكتريا Bacillus anthracis. Colonies showing viscous
+
+36
+00:02:31,170 --> 00:02:35,110
+or sticky growth, typical of an organism producing
+
+37
+00:02:35,110 --> 00:02:39,090
+large quantities of a carbohydrate capsule. قلنا
+
+38
+00:02:39,090 --> 00:02:43,470
+معظم البكتيريا اللي بتكون كابسول، الكولوني تبعها
+
+39
+00:02:43,470 --> 00:02:46,210
+على الطبق، في أننا  colony morphology، ممكن من
+
+40
+00:02:46,210 --> 00:02:51,210
+خلاله أتعرف على البكتيريا.  المستعمرات  على الطبق بعد
+
+41
+00:02:51,210 --> 00:02:54,430
+ما أنمي هذه البكتيريا بتكون طبيعتها viscous،
+
+42
+00:02:54,430 --> 00:02:57,830
+بتكون لزجة. على سبيل المثال، الكليبسيلة تعتبر
+
+43
+00:02:57,830 --> 00:03:02,510
+capsulated. لما تيجي تاخدي كولوني كليبسيلة باللوب،
+
+44
+00:03:02,510 --> 00:03:06,410
+بتلاقيها بتمغط. هذا نتيجة الكميات الكبيرة من
+
+45
+00:03:06,410 --> 00:03:09,970
+الكربوهيدرات اللي بتكون منها الكابسول. قلنا إن
+
+46
+00:03:09,970 --> 00:03:12,330
+الكابسول polysaccharide، يعني carbohydrate.
+
+47
+00:03:15,880 --> 00:03:20,620
+Capsules functions. شو وظائف الكبسول؟ أول وظيفة:
+
+48
+00:03:20,620 --> 00:03:24,740
+protect the cell from drying. هيحمي البكتيريا من
+
+49
+00:03:24,740 --> 00:03:29,180
+الجفاف. الكبسول هيعمل inhibit لـ water from
+
+50
+00:03:29,180 --> 00:03:33,160
+escaping into the environment. فبالتالي هيحمي الخلية
+
+51
+00:03:33,160 --> 00:03:37,280
+البكتيرية من الجفاف. protect the cell from
+
+52
+00:03:37,280 --> 00:03:42,070
+phagocytosis, being engulfed by white blood cells. بيحمي
+
+53
+00:03:42,070 --> 00:03:46,890
+الخلية من الـ phagocytosis، لأن الكبسول لزج، فلما
+
+54
+00:03:46,890 --> 00:03:50,490
+تيجي الـ phagocyte، اللي هي الـ White BCs، عشان
+
+55
+00:03:50,490 --> 00:03:55,590
+تعمل engulfment، تلتهم الخلية، بتروح الخلية بتهرب
+
+56
+00:03:55,590 --> 00:04:01,050
+لأن الخلية بتحتوي على الكبسول اللزج، فتهرب من جهاز
+
+57
+00:04:01,050 --> 00:04:04,510
+المناعة، فتهرب من الـ White BCs، وهذا يعتبر
+
+58
+00:04:04,510 --> 00:04:09,460
+virulence factor، عامل من عوامل الشراسة. ثالث وظيفة:
+
+59
+00:04:09,460 --> 00:04:12,840
+provide a food reserve when certain organic
+
+60
+00:04:12,840 --> 00:04:16,980
+compounds are in excess. بيقول: يعتبر مخزن للمواد
+
+61
+00:04:16,980 --> 00:04:21,700
+الغذائية في حال كان عندي فائض من هذه المواد، هتتخزن
+
+62
+00:04:21,700 --> 00:04:26,460
+في الكابسول. رابع وظيفة: يعتبر، قلنا إن هو virulence
+
+63
+00:04:26,460 --> 00:04:30,040
+factor، عامل من عوامل الشراسة، of pathogenic
+
+64
+00:04:30,040 --> 00:04:35,760
+microbes. خامس وظيفة: they serve as binding or
+
+65
+00:04:35,760 --> 00:04:39,380
+adhesion agents for sticking cells together or to
+
+66
+00:04:39,380 --> 00:04:44,200
+surfaces. وقول لي الوظيفة الخامسة هي الـ attachment. الـ
+
+67
+00:04:44,200 --> 00:04:47,800
+attachment، ممكن تلتصق الخلايا البكتيرية مع بعض
+
+68
+00:04:47,800 --> 00:04:52,440
+together or to the surface، أو على السطح. الكابسول
+
+69
+00:04:52,440 --> 00:04:56,740
+لزج، فبالتالي هيلزق البكتيريا على بعض مع بعض أو على
+
+70
+00:04:56,740 --> 00:05:01,100
+السطح. طيب، شو فايدة الـ attachment؟ بقول: عشان يصير في
+
+71
+00:05:01,100 --> 00:05:05,840
+عندي infection، عشان يصير المرض. أول خطوة لازم يصير
+
+72
+00:05:05,840 --> 00:05:10,120
+الـ attachment، تلتصق البكتيريا مع بعض أو تلتصق على
+
+73
+00:05:10,120 --> 00:05:14,420
+السطح، بعدين هيصير في عندي colonization، بعدين هيصير
+
+74
+00:05:14,420 --> 00:05:19,590
+الـ infection. ممكن، قلنا يكون على الأسطح زي السخور أو
+
+75
+00:05:19,590 --> 00:05:23,090
+على سطح الأسنان. في عندي بكتيريا Streptococcus
+
+76
+00:05:23,090 --> 00:05:27,350
+mutans، هذه البكتيريا بتعمل تسوس الأسنان، لأنه
+
+77
+00:05:27,350 --> 00:05:35,330
+موجودة في الفم، في وجود السكروز، تنتج الكابسول. هذه
+
+78
+00:05:35,330 --> 00:05:38,810
+الـ slide، هنتكلم عن عوامل لازم أنا أخدها بعين
+
+79
+00:05:38,810 --> 00:05:43,940
+الاعتبار لما بدي أصبغ الكابسول. أول معلومة: بكتيريا
+
+80
+00:05:43,940 --> 00:05:49,180
+الكابسول are non-ionic. قولي إن الكابسول  ليس لها شحنة،
+
+81
+00:05:49,180 --> 00:05:54,380
+so neither acidic nor basic stain will adhere to
+
+82
+00:05:54,380 --> 00:05:57,800
+their surface. عشان هيك، لا الـ acidic stain ولا الـ
+
+83
+00:05:57,800 --> 00:06:03,500
+basic stain هتقدر تلتصق بالكابسول. هتبقى هنعمل طريقة
+
+84
+00:06:03,500 --> 00:06:06,840
+عشان أقدر إني أنا أصبغ الكابسول. هنتعرف عليها.
+
+85
+00:06:06,840 --> 00:06:11,240
+Because most capsular materials are water soluble،
+
+86
+00:06:11,640 --> 00:06:15,320
+simple stains will not adhere to them. بقول لي معظم
+
+87
+00:06:15,320 --> 00:06:18,980
+مكونات الكابسول هي water soluble، عشان هيك الـ
+
+88
+00:06:18,980 --> 00:06:23,980
+simple stain مابتقدر تلتصق بالكابسول. الـ simple
+
+89
+00:06:23,980 --> 00:06:28,820
+stain، أنا خلال الـ simple stain بعمل washing بين كل
+
+90
+00:06:28,820 --> 00:06:33,040
+خطوة والتانية، فبالتالي الكابسول material هيتم
+
+91
+00:06:33,040 --> 00:06:36,700
+إزالته خلال الـ washing. يبقى خلال شغل في الـ capsular
+
+92
+00:06:36,700 --> 00:06:41,120
+stain، بدي أخد بعين الاعتبار rinsing the slide with
+
+93
+00:06:41,120 --> 00:06:45,000
+water. لازم إني أنا أتجنب إني أغسل الـ slide بالمية،
+
+94
+00:06:45,000 --> 00:06:50,000
+لأن الكابسول هيصير إزالته، dislodge. الكابسول هيتم
+
+95
+00:06:50,000 --> 00:06:57,700
+إزالته خلال عملية الـ washing. تالت معلومة: الـ older
+
+96
+00:06:57,700 --> 00:07:01,780
+cultures are most likely to exhibit capsule
+
+97
+00:07:01,780 --> 00:07:06,490
+production. بقول لي إنه لما بدي أنا أعمل capsular stain،
+
+98
+00:07:06,490 --> 00:07:10,310
+بأخذ الـ colony من older cultures. ليش؟ لأن الـ
+
+99
+00:07:10,310 --> 00:07:13,310
+older...لأن في الـ older cultures بتكون المواد
+
+100
+00:07:13,310 --> 00:07:18,010
+الغذائية بدأت تتخلص، والظروف بتصبح سيئة، فبالتالي
+
+101
+00:07:18,010 --> 00:07:22,730
+هيتكون عندي الكابسول. نفس الشيء بالنسبة للـ spore.
+
+102
+00:07:22,730 --> 00:07:27,130
+يُفضل أنها تكون older cultures، لأن بتتكون
+
+103
+00:07:27,130 --> 00:07:32,070
+الكابسول والـ spore في... لما تصير الظروف البيئية
+
+104
+00:07:32,070 --> 00:07:37,040
+صعبة. When performing a capsular stain on your
+
+105
+00:07:37,040 --> 00:07:40,740
+unknown, be sure the culture you take your sample
+
+106
+00:07:40,740 --> 00:07:44,980
+from is at least five days old. أقول لك عشان هيك لما
+
+107
+00:07:44,980 --> 00:07:50,040
+بدك إنتي تعملي أي capsular stain، تاخدي الكولوني من
+
+108
+00:07:50,040 --> 00:07:56,810
+culture، من مزرعة على الأقل يكون لها خمس أيام. أتربة
+
+109
+00:07:56,810 --> 00:08:01,590
+من السيراميك يمكن استخدامها في التحريك لتحسين صميم
+
+110
+00:08:01,590 --> 00:08:07,750
+الكبسول، وتجعله أسهل للتلاعب معه بمركب عدل، مثل
+
+111
+00:08:07,750 --> 00:08:14,420
+الميكروسكوب الضوئي، وقليلاً أثناء العمل. لأجل  السمير في الـ capsular
+
+112
+00:08:14,420 --> 00:08:19,100
+stain، ممكن أضيف drop of serum. drop of serum، بقول
+
+113
+00:08:19,100 --> 00:08:23,760
+هذا هيعمل على زيادة حجم الكابسول، enhance the size
+
+114
+00:08:23,760 --> 00:08:26,480
+of the capsule. يبقى هي وظيفة اللي هو الـ serum،
+
+115
+00:08:26,480 --> 00:08:31,700
+فبالتالي أنا هقدر أشوف الكابسول بشكل واضح، وقت ما أشـ
+
+116
+00:08:31,700 --> 00:08:35,500
+بحط اللي هو drop of serum. عندنا هنتعرف خلال الـ smear، أنا
+
+117
+00:08:35,500 --> 00:08:40,920
+بضيف كولوني، وهضيف معه اللي هو drop of serum.
+
+118
+00:08:44,070 --> 00:08:47,990
+كيف بدي أنا أصبغ الكابسول؟ في عندي أنا طريقتين
+
+119
+00:08:47,990 --> 00:08:51,670
+لصباغة الكابسول. الـ acid-fast، إذا بتتذكروا، باسـ
+
+120
+00:08:51,670 --> 00:08:56,910
+ليس فقط هذه طرق لصباغتها. بدي أنا أكون عارفة
+
+121
+00:08:56,910 --> 00:08:59,990
+كل الطرق، وشو الـ principle لكل طريقة، وكيف بتكون
+
+122
+00:08:59,990 --> 00:09:04,550
+نتيجة كل منها. بالنسبة للـ capsular stain، أول طريقة
+
+123
+00:09:04,550 --> 00:09:10,390
+اسمها الـ Gin’s stain method. بشكل مختصر، احنا قلنا إنه
+
+124
+00:09:10,390 --> 00:09:14,930
+الكابسول non-ionic، ما له شحنة. طب أنا كيف بدي أصبغ
+
+125
+00:09:14,930 --> 00:09:19,270
+هذا الـ capsule؟ بقول لنا في هذه الطريقة بنستخدم نوعين
+
+126
+00:09:19,270 --> 00:09:24,050
+من الصبغات. بنستخدم basic stain وبنستخدم acidic
+
+127
+00:09:24,050 --> 00:09:28,650
+stain. الـ basic stain هتصبغ البكتيريا. بنستخدم basic
+
+128
+00:09:28,650 --> 00:09:32,570
+stain زي الـ methylene blue أو الـ crystal violet. الـ
+
+129
+00:09:32,570 --> 00:09:35,610
+acidic stain هتصبغ... قلنا بتتذكروا في معمل الـ
+
+130
+00:09:35,610 --> 00:09:37,730
+symbiosis بتصبغ الـ background.
+
+131
+00:09:40,530 --> 00:09:43,830
+من الأمثلة على الـ acidic stain اللي ممكن أستخدمها،
+
+132
+00:09:43,830 --> 00:09:48,410
+زي الـ nigrosin أو الـ eosin. يبقى الـ basic stain
+
+133
+00:09:48,410 --> 00:09:53,350
+صبغت البكتيريا، الـ acidic stain هتصبغ الخلفية، الـ
+
+134
+00:09:53,350 --> 00:09:57,630
+background. بالتالي، هيبين الكابسول على شكل halo
+
+135
+00:09:57,630 --> 00:10:02,670
+area، على شكل فجوات حول البكتيريا. هيقدر أعرف هل هي
+
+136
+00:10:02,670 --> 00:10:06,650
+البكتيريا capsulated أو non-capsulated، بالرغم من
+
+137
+00:10:06,650 --> 00:10:11,710
+أن الكابسول ما له شحنة وما بقدر أصبغه. عشان هيك بسمي
+
+138
+00:10:11,710 --> 00:10:16,650
+الـ capsular stain، بسميها negative stain. إيش يعني
+
+139
+00:10:16,650 --> 00:10:21,590
+negative stain؟ يعني مش هتقدر تاخد الصبغة، لأنه
+
+140
+00:10:21,590 --> 00:10:25,450
+ما لها شحنة. مش هتقدر تاخد الصبغة سواء كانت acidic
+
+141
+00:10:25,450 --> 00:10:30,230
+أو basic stain. مش عشان الكبسولة بتحمل شحنة سالبة،
+
+142
+00:10:30,230 --> 00:10:33,070
+روحت سميتها negative stain. لأ، negative stain يعني
+
+143
+00:10:33,070 --> 00:10:40,040
+ما بتقدر تاخد الصبغة، لأن ما لها شحنة. وبالتالي، هذا السؤال
+
+144
+00:10:40,040 --> 00:10:44,480
+مهم جداً. ليش أنا بسمي الـ capsular stain بـ negative
+
+145
+00:10:44,480 --> 00:10:48,760
+stain؟ نقرأ
+
+146
+00:10:48,760 --> 00:10:52,620
+الـ slide. Gin’s method. هانكتب لي الـ principle تبع
+
+147
+00:10:52,620 --> 00:10:56,660
+هذه الطريقة. In this stain we use acidic and basic
+
+148
+00:10:56,660 --> 00:11:01,700
+dyes. بنستخدم acidic و basic dyes. Acidic dye as India
+
+149
+00:11:01,700 --> 00:11:04,740
+ink and nigrosin. بقول لي من الأمثلة على الـ acidic
+
+150
+00:11:04,740 --> 00:11:09,000
+dye، الـ India ink والـ nigrosin. Used to stain
+
+151
+00:11:09,000 --> 00:11:12,440
+the background of the slide. بقول لي بتستخدم لصباغة الـ
+
+152
+00:11:12,440 --> 00:11:17,180
+background، and basic stain. الـ basic stain، زي
+
+153
+00:11:17,180 --> 00:11:20,080
+الـ methylene blue أو الـ crystal violet، هتصبغ
+
+154
+00:11:20,080 --> 00:11:25,860
+الخلية البكتيرية. بقول لي الـ India ink هتعطي semi-
+
+155
+00:11:25,860 --> 00:11:30,180
+opaque background، هتعطي الخلفية شيء معتم، بالمقارنة،
+
+156
+00:11:30,180 --> 00:11:34,620
+إن الكابسول هيك هيبين، هيكون واضح، وهأقدر إني أنا
+
+157
+00:11:34,620 --> 00:11:37,360
+أشوفه بالرغم من إنه ما له شحنة.
+
+158
+00:11:42,080 --> 00:11:46,580
+بالنسبة لخطوات الشغل، أول شيء هعمل smear preparation.
+
+159
+00:11:46,580 --> 00:11:50,720
+طريقة الـ smear preparation في الـ Gin’s method
+
+160
+00:11:50,720 --> 00:11:55,100
+هتختلف عن أي smear preparation ثانية. بقول لي بجيب
+
+161
+00:11:55,100 --> 00:11:59,960
+slide، بحط على طرف الـ slide، مش بالنص، على طرف الـ
+
+162
+00:11:59,960 --> 00:12:03,280
+slide، drop of acidic stain.
+
+163
+00:12:08,580 --> 00:12:13,300
+الـ drop of India ink، أو ممكن نحكي بشكل عام اللي
+
+164
+00:12:13,300 --> 00:12:27,240
+هي acidic stain. acidic stain،
+
+165
+00:12:27,240 --> 00:12:31,560
+سواء India ink أو nigrosin، بحط drop من هذه الصبغة
+
+166
+00:12:31,560 --> 00:12:36,780
+على one end of the slide، على طرف الـ slide. بروح
+
+167
+00:12:36,780 --> 00:12:41,260
+بجيب اللي هو loop full of bacteria، البكتيريا اللي
+
+168
+00:12:41,260 --> 00:12:46,280
+بدي أشوف هل هي فيها كابسول أو لأ، وبعمل mix منيح
+
+169
+00:12:46,280 --> 00:12:50,140
+بين الـ drop high وما بين البكتيريا، لكن بدون ما
+
+170
+00:12:50,140 --> 00:12:53,760
+أعمل توزيع للـ drop في نفس المكان. بعمل mix.
+
+171
+00:13:01,510 --> 00:13:06,310
+بعدها، بروح بعد ما أعمل مكس منيح، بجيب slide ثاني، و
+
+172
+00:13:06,310 --> 00:13:09,550
+زي الـ blood smear، زي ما كنتوا بتعملوه في الـ blood
+
+173
+00:13:09,550 --> 00:13:13,610
+smear، كنتوا تحطوا drop من الـ blood وتفردوه. هي نفس
+
+174
+00:13:13,610 --> 00:13:17,530
+الشيء، لكن هي مش drop of blood، drop من الصبغة ومن
+
+175
+00:13:17,530 --> 00:13:20,350
+البكتيريا، أنا عاملها الـ home mix. بروح بجيب
+
+223
+00:17:14,210 --> 00:17:18,330
+نتيجة اللي هي الـ capsule stain بطريقة الـ Gins
+
+224
+00:17:18,330 --> 00:17:22,150
+method، الـ background انصبغت لونها أسود بالـ India
+
+225
+00:17:22,150 --> 00:17:27,090
+ink، البكتيريا انصبغت باللون الأزرق بالـ methylene blue
+
+226
+00:17:27,090 --> 00:17:31,970
+بالـ basic stain، لكن حوالين اللي هي البكتيريا فيها
+
+227
+00:17:31,970 --> 00:17:35,290
+عندي منطقة فاضية Halo area مش مصبوغة لأنها ما لها
+
+228
+00:17:35,290 --> 00:17:40,290
+شحنة، هي الـ capsule. هذا بالنسبة للـ Gins method
+
+229
+00:17:40,290 --> 00:17:44,370
+الـ Gins method، اتعرفنا على الـ principle تبعها،
+
+230
+00:17:44,370 --> 00:17:48,610
+خطواتها، وكيف بتكون النتيجة. الطريقة الثانية
+
+231
+00:17:48,610 --> 00:17:54,060
+اللي هي الـ Anthony's method. الـ Anthony's method، هعمل سمير
+
+232
+00:17:54,060 --> 00:17:57,460
+بـ preparation عادي زي أي stain، مش زي الـ Gins stain
+
+233
+00:17:57,460 --> 00:18:00,460
+باختلاف أن الـ Anthony's method، هعمل السمير
+
+234
+00:18:00,460 --> 00:18:04,760
+بـ preparation عادي، هعمل air-drying، مش هعمل heat
+
+235
+00:18:04,760 --> 00:18:09,180
+-fixation. وذكرنا الصباغة، بالنسبة للصباغة، هستخدم
+
+236
+00:18:09,180 --> 00:18:13,820
+two reagents، أول شيء الـ primary stain هيكون الـ
+
+237
+00:18:13,820 --> 00:18:17,680
+crystal violet only، which gives the bacterial cell
+
+238
+00:18:17,680 --> 00:18:21,750
+and its capsular material a dark purple. بقول لي هي
+
+239
+00:18:21,750 --> 00:18:27,230
+تعطي لون أرجواني داكن للـ capsular material للبكتيريا.
+
+240
+00:18:27,230 --> 00:18:31,090
+الـ capsular material، unlike the cell... أنكمل الـ
+
+241
+00:18:31,090 --> 00:18:34,630
+capsule is non-ionic and the primary stain cannot
+
+242
+00:18:34,630 --> 00:18:39,110
+adhere. بقول لي اتفقنا أن الكبسول non-ionic، ما له
+
+243
+00:18:39,110 --> 00:18:42,430
+شحنة، بالتالي الـ primary stain، الـ crystal violet مش
+
+244
+00:18:42,430 --> 00:18:50,470
+هتقدر تلتصق فيه. مدة الصباغة في الـ Crystal Violet في
+
+245
+00:18:50,470 --> 00:18:54,210
+خطوة الـ Anthony's Method، بقول لي من 4 لـ 7 دقائق
+
+246
+00:18:54,210 --> 00:18:59,470
+بخليها مغمورة بالـ Crystal Violet من 4 لـ 7 دقائق. في
+
+247
+00:18:59,470 --> 00:19:05,450
+الـ Gram Stain، لما أنا صبغت البكتيريا
+
+248
+00:19:05,450 --> 00:19:09,590
+بالـ Gram Stain، استخدمت الـ primary stain، الـ Crystal
+
+249
+00:19:09,590 --> 00:19:12,930
+Violet، لكن أنا خليت الـ Crystal Violet one minute.
+
+250
+00:19:13,340 --> 00:19:18,060
+لكن هنا بقول لي أني لازم أخليها من أربع لسبع
+
+251
+00:19:18,060 --> 00:19:22,020
+دقائق. بقول لي المدة الطويلة هذه بتكون as mordant.
+
+252
+00:19:22,020 --> 00:19:26,220
+يبقى بعد خطوة الـ primary stain، في عندي mordant، الـ
+
+253
+00:19:26,220 --> 00:19:31,520
+mordant هنا، المدة الطويلة للصبغة هتثبت الصبغة داخل
+
+254
+00:19:31,520 --> 00:19:38,930
+البكتيريا. بعد ما أنا أخلص، أخلص، أعمل اللي هو أخلص
+
+255
+00:19:38,930 --> 00:19:43,150
+الـ أربع لسبع دقائق، هل هغسل؟ بقول لي مش هتغسلي، لأن
+
+256
+00:19:43,150 --> 00:19:47,150
+إحنا قلنا أن الـ capsular material water soluble.
+
+257
+00:19:47,150 --> 00:19:51,510
+فبالتالي ممكن الكابسول هينزل خلال عملية الـ washing.
+
+258
+00:19:51,510 --> 00:19:56,340
+هيعطيني false negative result. هعمل خطوة الـ
+
+259
+00:19:56,340 --> 00:19:59,720
+decolorizer. الـ decolorizer، بقول لي اللي هو الـ
+
+260
+00:19:59,720 --> 00:20:04,220
+copper sulfate solution 20%، نسبته copper sulfate
+
+261
+00:20:04,220 --> 00:20:08,420
+solution. نحفظ التركيز في الـ Indus Bar. ماري معناه
+
+262
+00:20:08,420 --> 00:20:12,120
+الـ copper sulfate، هي كانت من مكونات الملوّنات الـ
+
+263
+00:20:12,120 --> 00:20:14,820
+green اللي كانت تعتبر water soluble، عشان ما أنا
+
+264
+00:20:14,820 --> 00:20:18,140
+خربطش. هنا copper sulfate اللي هو يعتبر الـ
+
+265
+00:20:18,140 --> 00:20:21,620
+decolorizer، في خطوة الـ Anthony's method لـ
+
+266
+00:20:21,620 --> 00:20:25,890
+capsular stain. بقول لي هذه المادة الكيميائية الـ
+
+267
+00:20:25,890 --> 00:20:30,830
+copper sulfate، بتعمل دور مزدوج، dual roles as both
+
+268
+00:20:30,830 --> 00:20:35,130
+the decolorizing agent and counterstain. يبقى
+
+269
+00:20:35,130 --> 00:20:38,950
+الـ primary stain، الـ crystal violet، الموردنت، المدة
+
+270
+00:20:38,950 --> 00:20:44,110
+الـ طويلة للصبغة من 4 إلى 7 دقائق، الـ decolorizer، و
+
+271
+00:20:44,110 --> 00:20:49,350
+الـ counterstain هيكون الـ 20% copper sulfate. طيب،
+
+272
+00:20:49,350 --> 00:20:55,130
+شو هي الوظيفة لـ الـ copper sulfate؟ بقول لي إنه
+
+273
+00:20:55,130 --> 00:20:59,030
+يعتبر كـ decolorizer، أو ممكن أنا أعتبره washer
+
+274
+00:20:59,030 --> 00:21:01,930
+كمان، لأنه مش هستخدم المية، لأن إحنا قلنا الـ
+
+275
+00:21:01,930 --> 00:21:06,190
+capsular material هي تعتبر water soluble، فيعتبر
+
+276
+00:21:06,190 --> 00:21:10,970
+كمان washer، it removes excess primary stain. بقول
+
+277
+00:21:10,970 --> 00:21:15,610
+لي، هيزيل أي زيادة من الـ primary stain. إحنا بعد أي
+
+278
+00:21:15,610 --> 00:21:19,050
+صبغة بنغسل، عشان أي شيء زيادة يروح. مش هستخدم الـ
+
+279
+00:21:19,050 --> 00:21:24,080
+water، فهو هيغسل كمان، يبقى هيستخدم كـ washer as well
+
+280
+00:21:24,080 --> 00:21:29,380
+as a decolorizer from the capsule only، and capsule appear
+
+281
+00:21:29,380 --> 00:21:32,600
+faint light blue. يبقى خلّينا في هذه كمان، بقول لك
+
+282
+00:21:32,600 --> 00:21:35,960
+هيزيل اللون من الكابسول، بشكل طبيعي اللون هيروح من
+
+283
+00:21:35,960 --> 00:21:41,480
+الكابسول، لأن الكابسول ما له شحنة، non-ionic. طيب هذه
+
+284
+00:21:41,480 --> 00:21:46,040
+وظيفته كـ decolorizer أو washer. بقول لي and capsule
+
+285
+00:21:46,040 --> 00:21:50,340
+appear light blue. طيب كيف الكابسول هياخد اللون
+
+286
+00:21:50,340 --> 00:21:55,030
+الأزرق الفاتح وهو ما له شحنة، ومرتبط بطريقة الصبغة.
+
+287
+00:21:55,030 --> 00:22:01,870
+قلنا الـ copper sulfate هيشتغل كـ counterstain، كيف
+
+288
+00:22:01,870 --> 00:22:07,670
+هيشتغل الـ copper sulfate كـ counterstain ويعطي الكابسول
+
+289
+00:22:07,670 --> 00:22:11,390
+اللون الأزرق بالرغم من أنه ما له شحنة. بقول لي
+
+290
+00:22:11,390 --> 00:22:15,470
+الـ copper sulfate هيشتغل كـ counterstain من خلال أنه
+
+291
+00:22:15,470 --> 00:22:20,200
+هيعمل فرق في الأيونات. يبقى هذا الفرق هيعطي
+
+292
+00:22:20,200 --> 00:22:24,820
+الكابسول بعض من الأيونات، يعني هيعطي بعض من الشحنات
+
+293
+00:22:24,820 --> 00:22:29,960
+بالتالي الصبغة هترتبط فيه، وهتنطق... هتطلع خارج
+
+294
+00:22:29,960 --> 00:22:34,600
+الخلية، وهتنتقل للكابسول، هتعطيه لون أزرق فاتح. يبقى
+
+295
+00:22:34,600 --> 00:22:40,740
+هيك اشتغل الـ... الـ copper sulfate كـ counterstain،
+
+296
+00:22:40,740 --> 00:22:44,460
+أنه أعطى الكابسول اللون الـ light blue. اشتغل كـ
+
+297
+00:22:44,460 --> 00:22:47,920
+decolorizing agent من خلال أنه زال اللون من
+
+298
+00:22:47,920 --> 00:22:53,360
+الكابسول. يبقى الـ copper sulfate اشتغل كـ
+
+299
+00:22:53,360 --> 00:22:56,540
+counterstain، مش يعني counterstain، يعني ميّز لي
+
+300
+00:22:56,540 --> 00:22:59,900
+حاجة عن حاجة، الخلية البكتيرية أعطاها اللون الـ
+
+301
+00:22:59,900 --> 00:23:04,100
+purple بشكل طبيعي، لكن الكابسول أعطاها اللون الفاتح
+
+302
+00:23:04,100 --> 00:23:09,030
+الـ light blue. يبقى هيك كان كـ counterstain. بالنسبة
+
+303
+00:23:09,030 --> 00:23:15,050
+للخطوات، أول خطوة، قلنا aseptically transfer a loop
+
+304
+00:23:15,050 --> 00:23:18,410
+full of culture within a inoculating loop to the
+
+305
+00:23:18,410 --> 00:23:22,390
+slide. بدي أعمل smear preparation aseptically، يعني
+
+306
+00:23:22,390 --> 00:23:26,610
+بدي أمنع أي شيء. كنت مانعش، أن أستخدم sterile loop،
+
+307
+00:23:26,610 --> 00:23:32,150
+أشتغل في مكان معقّم. لو استخدمت مثلًا البكتيريا من
+
+308
+00:23:32,150 --> 00:23:36,870
+أطباق، تكون الـ normal saline sterile. كلها aseptic
+
+309
+00:23:36,870 --> 00:23:40,290
+technique عشان أمنع الـ contamination. الخطوة الثانية،
+
+310
+00:23:40,290 --> 00:23:45,050
+هعمل air drying، بعدها مش هعمل heat fixation، لأن
+
+311
+00:23:45,050 --> 00:23:52,270
+اتفقنا أنه fragile. إيش الـ fragile يعني؟ الكابسول
+
+312
+00:23:52,270 --> 00:23:56,490
+ممكن إنه يتكسر بالحرارة، فبالتالي يعطيني false
+
+313
+00:23:56,490 --> 00:24:02,690
+negative result. في سبب ثاني، ليش أنا ما بديش أعمل
+
+314
+00:24:02,690 --> 00:24:06,950
+heat fixation في الـ slide. الأطباء in this procedure
+
+315
+00:24:06,950 --> 00:24:11,410
+السمير shouldn't be heat fixed، since shrinkage
+
+316
+00:24:11,410 --> 00:24:15,210
+is likely to occur and create a clear zone around
+
+317
+00:24:15,210 --> 00:24:19,070
+the bacteria which can be mistaken for a capsule.
+
+318
+00:24:19,070 --> 00:24:24,110
+بقول لي أنا لو أنا رحت عملت heat fixation، ممكن
+
+319
+00:24:24,110 --> 00:24:28,590
+الخلية البكتيرية يصير لها shrinking، لما تنكمش
+
+320
+00:24:28,590 --> 00:24:32,190
+الخلية البكتيرية، هيكون في مكان فاضي، create clear
+
+321
+00:24:32,190 --> 00:24:36,790
+zone around the bacterium. فممكن أنا أفكر الـ clear
+
+322
+00:24:36,790 --> 00:24:40,970
+zone هذا هو الكابسول، لكن مش كابسول، هيعطيني false
+
+323
+00:24:40,970 --> 00:24:44,810
+positive result. يبقى في عندي سببين لعدم عمل خطوة
+
+324
+00:24:44,810 --> 00:24:48,650
+الـ heat fixation، السبب الأول، لأنه fragile الكابسول،
+
+325
+00:24:48,650 --> 00:24:51,970
+فبالتالي ممكن يتكسر بالحرارة ويعطيني false
+
+326
+00:24:51,970 --> 00:24:56,360
+negative result. لكن السبب الثاني، ممكن البكتيريا
+
+327
+00:24:56,360 --> 00:24:59,560
+يصير لها shrinking، فبالتالي تكون الـ clear zone،
+
+328
+00:24:59,560 --> 00:25:04,160
+أفكر هو الكابسول، فهي يعطيني false positive result.
+
+329
+00:25:04,160 --> 00:25:10,120
+نكمل الخطوات، الخطوة اللي بعدها، أعمل staining بالـ
+
+330
+00:25:10,120 --> 00:25:12,780
+primary stain، اللي هي الـ crystal violet. يبقى الـ
+
+331
+00:25:12,780 --> 00:25:16,340
+crystal violet هي الـ primary stain، هخليها من أربع
+
+332
+00:25:16,340 --> 00:25:20,000
+لسبع دقائق. وقلنا هي الأربع لسبع دقائق، هتكون
+
+333
+00:25:20,000 --> 00:25:25,270
+الموردنت. عشرين في المئة. الخطوة التي سأقوم بها هي كـ
+
+334
+00:25:25,270 --> 00:25:29,270
+decolorizer أو washer، وهي نفس الشيء، كـ counter
+
+335
+00:25:29,270 --> 00:25:32,970
+stain. إذا ملاحظين، ما كان في خطوة اللي هو هغسل
+
+336
+00:25:32,970 --> 00:25:36,090
+بالمية، لأن إحنا قلنا الـ capsular material هي
+
+337
+00:25:36,090 --> 00:25:40,990
+تعتبر water soluble. هعمل air drying، وهشوفها تحت
+
+338
+00:25:40,990 --> 00:25:45,970
+الميكروسكوب على الـ oil immersion. يبقى نكتب
+
+339
+00:25:45,970 --> 00:25:49,830
+الـ Crystal
+
+340
+00:25:49,830 --> 00:25:50,490
+Violet،
+
+341
+00:25:54,140 --> 00:25:57,560
+الـ أربع على سبعة هتكون mordant، والعشرين في المئة
+
+342
+00:25:57,560 --> 00:26:04,980
+هيكون copper sulfate، هيكون decolorizer and
+
+343
+00:26:04,980 --> 00:26:07,300
+counterstain.
+
+344
+00:27:30,620 --> 00:27:34,540
+يبقى في هذه الطريقة، الـ Anthony's method، تعرفنا على الـ
+
+345
+00:27:34,540 --> 00:27:37,960
+primary stain، الموردنت، الـ decolorizer، والـ
+
+346
+00:27:37,960 --> 00:27:41,660
+counterstain. في طريقة الـ Gins method، ما استخدمت
+
+347
+00:27:41,660 --> 00:27:44,520
+ولا primary ولا mordant ولا decolorizer أو counter
+
+348
+00:27:44,520 --> 00:27:48,660
+stain. كانت الفكرة إنه أنا بستخدم أسدك Stain كانت
+
+349
+00:27:48,660 --> 00:28:05,300
+أو بازرق Stain، فبالتالي هيبين عندي الـ capsule. هذه
+
+350
+00:28:05,300 --> 00:28:11,140
+الصورة بتوضح بعض الخطوات. في الصورة، قلنا crystal
+
+351
+00:28:11,140 --> 00:28:14,160
+violet primary stain، الأربع على سبعة minutes، هتكون
+
+352
+00:28:14,160 --> 00:28:19,030
+mordant. بعدها مش هغسل بالمية، هعمل اللي هغسل بيه
+
+353
+00:28:19,030 --> 00:28:22,030
+الـ copper sulfate 20%، copper sulfate
+
+354
+00:28:22,030 --> 00:28:27,030
+هتكون كـ decolorizer والـ counterstain. هذا
+
+355
+00:28:27,030 --> 00:28:31,310
+بالنسبة لنتائج اللي هي الـ capsular stain، باستخدام
+
+356
+00:28:31,310 --> 00:28:35,950
+طريقة الـ Anthony's method، الـ capsule، نلاحظ أن هو
+
+357
+00:28:36,540 --> 00:28:40,540
+البكتيريا ماخدة اللون الـ dark purple عادي، لأنه
+
+358
+00:28:40,540 --> 00:28:43,660
+هتاخد الـ crystal violet بشكل طبيعي، لأنه هي basic
+
+359
+00:28:43,660 --> 00:28:48,160
+stain، هتصبغ البكتيريا. الكابسول هياخد لون
+
+360
+00:28:48,160 --> 00:28:52,000
+فاتح كثير، لون موف فاتح، أو ممكن نعتبره أزرق فاتح
+
+361
+00:28:52,000 --> 00:28:55,520
+لأنه إحنا قلنا الـ copper sulfate هيعمل فرق في الأيونات،
+
+362
+00:28:55,520 --> 00:28:59,340
+هيعطي الكابسول أيونات، يعني هيصير له شحنة، فبالتالي
+
+363
+00:28:59,340 --> 00:29:05,080
+هتطلع جزء من الصبغة لخارج البكتيريا، وهتتلون باللون
+
+364
+00:29:05,430 --> 00:29:09,450
+الـ light blue أو الـ light purple. بدنا نفرّق
+
+365
+00:29:09,450 --> 00:29:13,070
+بين نتيجة الـ Anthony's method والـ Gins method، الـ
+
+366
+00:29:13,070 --> 00:29:16,790
+result بتختلف حسب الطريقة. بدنا نكون عارفين كل
+
+367
+00:29:16,790 --> 00:29:22,010
+طريقة، شو الـ principle تبعها، إيش الـ procedure لكل
+
+368
+00:29:22,010 --> 00:29:27,250
+طريقة، وكيف بتكون النتيجة في كل طريقة. هذا بالنسبة
+
+369
+00:29:27,250 --> 00:29:28,970
+لـ capsular stain.
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/XkWBgngbjy4_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/XkWBgngbjy4_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..ddf1e4efc6120c67d341323025cf7516af9cbb11
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/XkWBgngbjy4_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 2661, "start": 0.69, "end": 26.61, "text": "تاني نوع من أنواع الـ special stain الـ capsular stain قلنا إنه الـ capsule أحد الـ special component اللي عدم وجودها لا يؤثر على الخلية البكتيرية يعني في عندي بكتيريا capsulated وفي بكتيريا non-capsulated capsule are gelatinous outer layer structure هو طبقة خارجية secreted by bacterial cells that lay outside of an organism cell wall", "tokens": [2655, 7649, 1829, 8717, 45367, 9154, 14739, 14407, 3615, 2423, 39184, 2121, 16441, 2423, 39184, 13855, 1040, 16441, 12174, 1211, 8315, 36145, 3224, 2423, 39184, 29247, 5551, 24401, 2423, 39184, 2121, 6542, 13672, 1829, 6225, 40448, 49610, 23328, 11296, 20193, 7251, 33604, 49115, 15844, 33962, 1211, 10632, 29739, 4117, 2655, 13546, 10632, 37495, 22653, 8978, 18871, 16254, 4724, 4117, 2655, 13546, 25528, 13855, 6987, 4032, 41185, 4724, 4117, 2655, 13546, 25528, 2107, 12, 496, 1878, 6987, 29247, 366, 45174, 563, 10847, 4583, 3877, 31439, 23032, 3555, 28671, 16490, 9640, 7435, 10632, 4054, 292, 538, 35632, 5438, 300, 2360, 2380, 295, 364, 24128, 2815, 2929], "avg_logprob": -0.17946429139091855, "compression_ratio": 1.6867924528301887, "no_speech_prob": 0.0, "words": [{"start": 0.69, "end": 1.09, "word": "تاني", "probability": 0.8611653645833334}, {"start": 1.09, "end": 1.35, "word": " نوع", "probability": 0.989013671875}, {"start": 1.35, "end": 1.53, "word": " من", "probability": 0.98828125}, {"start": 1.53, "end": 1.95, "word": " أنواع", "probability": 0.9443359375}, {"start": 1.95, "end": 2.19, "word": " الـ", "probability": 0.943603515625}, {"start": 2.19, "end": 2.43, "word": " special", "probability": 0.22998046875}, {"start": 2.43, "end": 2.99, "word": " stain", "probability": 0.415283203125}, {"start": 2.99, "end": 3.23, "word": " الـ", "probability": 0.7049560546875}, {"start": 3.23, "end": 3.83, "word": " capsular", "probability": 0.617919921875}, {"start": 3.83, "end": 4.29, "word": " stain", "probability": 0.9248046875}, {"start": 4.29, "end": 4.71, "word": " قلنا", "probability": 0.8186848958333334}, {"start": 4.71, "end": 4.95, "word": " إنه", "probability": 0.501708984375}, {"start": 4.95, "end": 5.05, "word": " الـ", "probability": 0.703857421875}, {"start": 5.05, "end": 5.41, "word": " capsule", "probability": 0.80322265625}, {"start": 5.41, "end": 5.97, "word": " أحد", "probability": 0.982666015625}, {"start": 5.97, "end": 6.21, "word": " الـ", "probability": 0.902587890625}, {"start": 6.21, "end": 6.49, "word": " special", "probability": 0.9111328125}, {"start": 6.49, "end": 7.15, "word": " component", "probability": 0.89892578125}, {"start": 7.15, "end": 7.47, "word": " اللي", "probability": 0.881103515625}, {"start": 7.47, "end": 7.73, "word": " عدم", "probability": 0.69970703125}, {"start": 7.73, "end": 8.25, "word": " وجودها", "probability": 0.9241536458333334}, {"start": 8.25, "end": 8.43, "word": " لا", "probability": 0.8837890625}, {"start": 8.43, "end": 8.89, "word": " يؤثر", "probability": 0.98828125}, {"start": 8.89, "end": 9.15, "word": " على", "probability": 0.9482421875}, {"start": 9.15, "end": 9.77, "word": " الخلية", "probability": 0.9742838541666666}, {"start": 9.77, "end": 10.71, "word": " البكتيرية", "probability": 0.85966796875}, {"start": 10.71, "end": 11.23, "word": " يعني", "probability": 0.904052734375}, {"start": 11.23, "end": 11.37, "word": " في", "probability": 0.806640625}, {"start": 11.37, "end": 11.61, "word": " عندي", "probability": 0.779541015625}, {"start": 11.61, "end": 12.13, "word": " بكتيريا", "probability": 0.97177734375}, {"start": 12.13, "end": 12.87, "word": " capsulated", "probability": 0.772216796875}, {"start": 12.87, "end": 13.19, "word": " وفي", "probability": 0.773681640625}, {"start": 13.19, "end": 13.75, "word": " بكتيريا", "probability": 0.9869140625}, {"start": 13.75, "end": 14.07, "word": " non", "probability": 0.7958984375}, {"start": 14.07, "end": 15.03, "word": "-capsulated", "probability": 0.78631591796875}, {"start": 15.03, "end": 15.99, "word": " capsule", "probability": 0.380859375}, {"start": 15.99, "end": 16.59, "word": " are", "probability": 0.60009765625}, {"start": 16.59, "end": 17.47, "word": " gelatinous", "probability": 0.88525390625}, {"start": 17.47, "end": 18.01, "word": " outer", "probability": 0.88134765625}, {"start": 18.01, "end": 18.53, "word": " layer", "probability": 0.845703125}, {"start": 18.53, "end": 19.21, "word": " structure", "probability": 0.90625}, {"start": 19.21, "end": 19.41, "word": " هو", "probability": 0.7744140625}, {"start": 19.41, "end": 19.83, "word": " طبقة", "probability": 0.9749348958333334}, {"start": 19.83, "end": 20.63, "word": " خارجية", "probability": 0.9403076171875}, {"start": 20.63, "end": 21.79, "word": " secreted", "probability": 0.9208984375}, {"start": 21.79, "end": 22.01, "word": " by", "probability": 0.95166015625}, {"start": 22.01, "end": 22.69, "word": " bacterial", "probability": 0.82421875}, {"start": 22.69, "end": 23.13, "word": " cells", "probability": 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الكابسول هيصله ازالة dislodge الكابسول هيتم ازالته خلال عملية ال washing تالت معلومة ال older culture are most likely to exhibit capsule production بقوللي انه لما بدي انا اعمل", "tokens": [3555, 16254, 1975, 9778, 3215, 4724, 40228, 22653, 42963, 34268, 3555, 9640, 367, 1292, 278, 264, 4137, 365, 1281, 5296, 31377, 2304, 16472, 1829, 1975, 8315, 1975, 2655, 7435, 1863, 3555, 3224, 16472, 1829, 1975, 17082, 3794, 1211, 2423, 4137, 20666, 2304, 1829, 3224, 5296, 7649, 33251, 16758, 3794, 12610, 39896, 36520, 3224, 1975, 11622, 6027, 3660, 43186, 19315, 33251, 16758, 3794, 12610, 39896, 39237, 1975, 11622, 6027, 47395, 16490, 1211, 6027, 6225, 42213, 10632, 2423, 13836, 6055, 6027, 2655, 20449, 1211, 20498, 3660, 2423, 4906, 3713, 366, 881, 3700, 281, 20487, 29247, 4265, 4724, 39648, 20292, 16472, 3224, 5296, 15042, 4724, 16254, 1975, 8315, 1975, 25957, 1211], "avg_logprob": -0.18721329728397754, "compression_ratio": 1.64, "no_speech_prob": 5.960464477539062e-07, "words": [{"start": 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stain صبغت البكتيريا ال acidic stain هتصبغها الخلفية ال background بالتالي هيبين الكابسول على شكل hello area على شكل فجوات حول البكتيريا هيقدرت أعرف هل هي البكتيريا capsulated أو non capsulated بالرغم من أن الكابسول ما له شحنة وما بقدر أصبغه", "tokens": [27842, 16247, 2304, 12984, 37977, 15844, 2423, 39184, 5097, 327, 299, 745, 491, 13672, 1829, 3714, 43020, 44713, 9778, 40448, 11296, 30767, 1829, 28239, 4117, 32887, 3794, 9957, 34051, 2423, 7649, 31371, 995, 9957, 4117, 7251, 3555, 4587, 7578, 2423, 3875, 16441, 20328, 3555, 17082, 2655, 29739, 4117, 2655, 13546, 25528, 2423, 8258, 299, 16441, 8032, 2655, 9381, 3555, 17082, 11296, 33962, 46538, 10632, 2423, 3678, 20666, 2655, 6027, 1829, 39896, 3555, 9957, 33251, 16758, 3794, 12610, 15844, 13412, 28820, 7751, 1859, 15844, 13412, 28820, 6156, 7435, 2407, 9307, 11331, 12610, 29739, 4117, 2655, 13546, 25528, 39896, 28543, 43500, 5551, 3615, 28480, 8032, 1211, 39896, 29739, 4117, 2655, 13546, 25528, 13855, 6987, 34051, 2107, 13855, 6987, 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"temperature": 1.0}, {"id": 24, "seek": 63903, "start": 610.23, "end": 639.03, "text": "عشان هيك بسمي الـ Capsular Stain بسميها Negative Stain إيش يعني Negative Stain؟ يعني مش هتقدر تاخد الصبغة لأنه مايلها شحنة مش هتقدر تاخد الصبغة سواء كانت Acidic أو Basic Stain مش عشان الكابسولة بيحمل شحنة سالبة روحت سماتها Negative Stain لأ Negative Stain يعني ماتقدر تاخد الصبغة لأنه مايلها شحنة", "tokens": [3615, 8592, 7649, 39896, 4117, 4724, 38251, 1829, 2423, 39184, 383, 2382, 1040, 745, 491, 4724, 38251, 1829, 11296, 43230, 745, 491, 11933, 1829, 8592, 37495, 22653, 43230, 745, 491, 22807, 37495, 22653, 37893, 8032, 2655, 28543, 2288, 6055, 47283, 3215, 31767, 3555, 17082, 3660, 5296, 33456, 3224, 19446, 26895, 11296, 13412, 5016, 1863, 3660, 37893, 8032, 2655, 28543, 2288, 6055, 47283, 3215, 31767, 3555, 17082, 3660, 8608, 2407, 16606, 25961, 2655, 5097, 327, 299, 34051, 31598, 745, 491, 37893, 6225, 8592, 7649, 33251, 16758, 3794, 12610, 3660, 4724, 1829, 35571, 1211, 13412, 5016, 1863, 3660, 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فهيعتبر", "probability": 0.86318359375}, {"start": 1266.19, "end": 1266.59, "word": " كمان", "probability": 0.9892578125}, {"start": 1266.59, "end": 1267.03, "word": " washer", "probability": 0.93359375}, {"start": 1267.03, "end": 1268.59, "word": " it", "probability": 0.67041015625}, {"start": 1268.59, "end": 1269.27, "word": " removes", "probability": 0.88037109375}, {"start": 1269.27, "end": 1269.77, "word": " excess", "probability": 0.89892578125}, {"start": 1269.77, "end": 1270.33, "word": " primary", "probability": 0.79443359375}, {"start": 1270.33, "end": 1270.73, "word": " stain", "probability": 0.822265625}, {"start": 1270.73, "end": 1270.97, "word": " بقول", "probability": 0.67333984375}, {"start": 1270.97, "end": 1271.03, "word": " لي", "probability": 0.54931640625}, {"start": 1271.03, "end": 1271.73, "word": " هيزيل", "probability": 0.9025065104166666}, {"start": 1271.73, "end": 1272.27, "word": " أي", "probability": 0.474609375}, {"start": 1272.27, "end": 1272.83, "word": 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"word": " إش", "probability": 0.893798828125}, {"start": 1277.29, "end": 1277.73, "word": " زيادة", "probability": 0.9356689453125}, {"start": 1277.73, "end": 1278.25, "word": " يروحها", "probability": 0.84197998046875}, {"start": 1278.25, "end": 1278.37, "word": " مش", "probability": 0.8896484375}, {"start": 1278.37, "end": 1278.89, "word": " هستخدم", "probability": 0.9827880859375}, {"start": 1278.89, "end": 1279.05, "word": " ال", "probability": 0.98974609375}, {"start": 1279.05, "end": 1279.33, "word": " water", "probability": 0.67919921875}, {"start": 1279.33, "end": 1279.75, "word": " فهو", "probability": 0.9759114583333334}, {"start": 1279.75, "end": 1280.31, "word": " هيغسل", "probability": 0.9654541015625}, {"start": 1280.31, "end": 1280.81, "word": " كمان", "probability": 0.99609375}, {"start": 1280.81, "end": 1281.21, "word": " يبقى", "probability": 0.92822265625}, {"start": 1281.21, "end": 1281.89, "word": " هيستخدم", "probability": 0.9122314453125}, {"start": 1281.89, 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الكابسول", "probability": 0.97998046875}, {"start": 1296.46, "end": 1296.74, "word": " لأن", "probability": 0.78564453125}, {"start": 1296.74, "end": 1297.34, "word": " الكابسول", "probability": 0.9034423828125}, {"start": 1297.34, "end": 1297.96, "word": " مايله", "probability": 0.7474772135416666}, {"start": 1297.96, "end": 1298.48, "word": " شحنة", "probability": 0.8251953125}, {"start": 1298.48, "end": 1298.66, "word": " non", "probability": 0.5029296875}, {"start": 1298.66, "end": 1299.8, "word": "-ionic", "probability": 0.83837890625}, {"start": 1299.8, "end": 1301.1, "word": " طيب", "probability": 0.92041015625}, {"start": 1301.1, "end": 1301.48, "word": " هاي", "probability": 0.6834716796875}, {"start": 1301.48, "end": 1302.22, "word": " وظيفته", "probability": 0.9019775390625}, {"start": 1302.22, "end": 1302.8, "word": " ك", "probability": 0.9892578125}, {"start": 1302.8, "end": 1303.9, "word": " decolorizer", "probability": 0.6378580729166666}, {"start": 1303.9, "end": 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capsule بقوللي انا لو انا روحت عملت heat fixation ممكن الخلية البكتيرية يصير لها shrinking", "tokens": [20292, 8592, 41850, 4724, 16254, 8592, 1975, 25957, 1211, 3738, 3191, 399, 8978, 2423, 4137, 16247, 36150, 294, 341, 10747, 21136, 2304, 13546, 4659, 380, 312, 2045, 3738, 6806, 1670, 23060, 609, 307, 3700, 281, 5160, 293, 1884, 257, 1850, 6668, 926, 264, 11763, 597, 393, 312, 21333, 337, 257, 29247, 4724, 39648, 20292, 1975, 8315, 45164, 1975, 8315, 12602, 2407, 33753, 6225, 42213, 2655, 3738, 3191, 399, 3714, 43020, 33962, 1211, 10632, 29739, 4117, 2655, 13546, 10632, 7251, 9381, 13546, 5296, 11296, 41684], "avg_logprob": -0.18060661343967213, "compression_ratio": 1.4901185770750989, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 1441.17, "end": 1441.53, "word": "ليش", "probability": 0.7490234375}, {"start": 1441.53, "end": 1441.69, "word": " أنا", "probability": 0.76806640625}, {"start": 1441.69, "end": 1442.21, "word": " بديش", "probability": 0.7083333333333334}, 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فبالتالي", "probability": 0.9812825520833334}, {"start": 1739.34, "end": 1740.0, "word": " هتطلع", "probability": 0.97978515625}, {"start": 1740.0, "end": 1740.6, "word": " جزء", "probability": 0.7389322916666666}, {"start": 1740.6, "end": 1740.9, "word": " من", "probability": 0.9931640625}, {"start": 1740.9, "end": 1742.04, "word": " الصبغة", "probability": 0.89501953125}, {"start": 1742.04, "end": 1743.04, "word": " لخرج", "probability": 0.7490234375}, {"start": 1743.04, "end": 1743.84, "word": " البكتيريا", "probability": 0.96982421875}, {"start": 1743.84, "end": 1744.52, "word": " وهتتلون", "probability": 0.91181640625}, {"start": 1744.52, "end": 1745.08, "word": " باللون", "probability": 0.9874674479166666}], "temperature": 1.0}, {"id": 65, "seek": 176897, "start": 1745.43, "end": 1768.97, "text": "الـ Light Blue أو الـ Light Purple بدنا نفرق بنتيجة الـ Anthony's method والـ Gen's method ال result بتختلف حسب الطريقة بدنا نكون عارفين كل طريقة شو ال principle تبعها ايش ال procedure لكل طريقة وكيف بتكون النتيجة في كل طريقة هذا بالنسبة لـ Capsular Style", "tokens": [6027, 39184, 8279, 8510, 34051, 2423, 39184, 8279, 28483, 47525, 8315, 8717, 5172, 2288, 4587, 44945, 31371, 7435, 3660, 2423, 39184, 5130, 71, 2526, 311, 3170, 16070, 39184, 3632, 311, 3170, 2423, 1874, 39894, 46456, 46538, 11331, 35457, 41950, 16572, 28671, 47525, 8315, 8717, 30544, 6225, 9640, 5172, 9957, 28242, 23032, 16572, 28671, 13412, 2407, 2423, 8665, 6055, 3555, 3615, 11296, 1975, 1829, 8592, 2423, 10747, 5296, 28820, 23032, 16572, 28671, 4032, 4117, 33911, 39894, 30544, 28239, 31371, 7435, 3660, 8978, 28242, 23032, 16572, 28671, 23758, 20666, 1863, 35457, 3660, 5296, 39184, 383, 2382, 1040, 27004], "avg_logprob": -0.24097937652745197, "compression_ratio": 1.6754385964912282, "no_speech_prob": 4.410743713378906e-06, "words": [{"start": 1745.43, "end": 1745.79, "word": "الـ", "probability": 0.84326171875}, {"start": 1745.79, "end": 1746.15, "word": " Light", "probability": 0.53125}, {"start": 1746.15, "end": 1746.59, "word": " Blue", "probability": 0.64453125}, {"start": 1746.59, "end": 1746.95, "word": " أو", "probability": 0.94970703125}, {"start": 1746.95, "end": 1747.21, "word": " الـ", "probability": 0.909912109375}, {"start": 1747.21, "end": 1747.61, "word": " Light", "probability": 0.94287109375}, {"start": 1747.61, "end": 1748.03, "word": " Purple", "probability": 0.96435546875}, {"start": 1748.03, "end": 1748.95, "word": " بدنا", "probability": 0.56365966796875}, {"start": 1748.95, "end": 1749.45, "word": " نفرق", "probability": 0.9775390625}, {"start": 1749.45, "end": 1750.35, "word": " بنتيجة", "probability": 0.8819580078125}, {"start": 1750.35, "end": 1750.51, "word": " الـ", "probability": 0.7978515625}, {"start": 1750.51, "end": 1751.17, "word": " Anthony's", "probability": 0.62005615234375}, {"start": 1751.17, "end": 1751.53, "word": " method", "probability": 0.76513671875}, {"start": 1751.53, "end": 1751.85, "word": " والـ", "probability": 0.679443359375}, {"start": 1751.85, "end": 1752.13, "word": " Gen's", "probability": 0.5157470703125}, {"start": 1752.13, "end": 1752.59, "word": " method", "probability": 0.95068359375}, {"start": 1752.59, "end": 1753.07, "word": " ال", "probability": 0.8623046875}, {"start": 1753.07, "end": 1753.65, "word": " result", "probability": 0.418212890625}, {"start": 1753.65, "end": 1754.41, "word": " بتختلف", "probability": 0.9690755208333334}, {"start": 1754.41, "end": 1755.17, "word": " حسب", "probability": 0.95849609375}, {"start": 1755.17, "end": 1755.75, "word": " الطريقة", "probability": 0.98779296875}, {"start": 1755.75, "end": 1755.95, "word": " بدنا", "probability": 0.7607421875}, {"start": 1755.95, "end": 1756.09, "word": " نكون", "probability": 0.983642578125}, {"start": 1756.09, "end": 1756.53, "word": " عارفين", "probability": 0.9910888671875}, {"start": 1756.53, "end": 1756.79, "word": " كل", "probability": 0.74853515625}, {"start": 1756.79, "end": 1757.27, "word": " طريقة", "probability": 0.9886067708333334}, {"start": 1757.27, "end": 1757.45, "word": " شو", "probability": 0.91455078125}, {"start": 1757.45, "end": 1757.57, "word": " ال", "probability": 0.9638671875}, {"start": 1757.57, "end": 1758.09, "word": " principle", "probability": 0.72314453125}, {"start": 1758.09, "end": 1758.89, "word": " تبعها", "probability": 0.9425048828125}, {"start": 1758.89, "end": 1759.47, "word": " ايش", "probability": 0.6288655598958334}, {"start": 1759.47, "end": 1759.61, "word": " ال", "probability": 0.9921875}, {"start": 1759.61, "end": 1760.17, "word": " procedure", "probability": 0.96826171875}, {"start": 1760.17, "end": 1762.01, "word": " لكل", "probability": 0.48095703125}, {"start": 1762.01, "end": 1763.33, "word": " طريقة", "probability": 0.9578450520833334}, {"start": 1763.33, "end": 1764.05, "word": " وكيف", "probability": 0.78955078125}, {"start": 1764.05, "end": 1764.47, "word": " بتكون", "probability": 0.84375}, {"start": 1764.47, "end": 1765.05, "word": " النتيجة", "probability": 0.9686279296875}, {"start": 1765.05, "end": 1765.21, "word": " في", "probability": 0.85693359375}, {"start": 1765.21, "end": 1765.45, "word": " كل", "probability": 0.98193359375}, {"start": 1765.45, "end": 1766.15, "word": " طريقة", "probability": 0.9832356770833334}, {"start": 1766.15, "end": 1766.63, "word": " هذا", "probability": 0.87109375}, {"start": 1766.63, "end": 1767.25, "word": " بالنسبة", "probability": 0.9837646484375}, {"start": 1767.25, "end": 1767.59, "word": " لـ", "probability": 0.742919921875}, {"start": 1767.59, "end": 1768.37, "word": " Capsular", "probability": 0.731201171875}, {"start": 1768.37, "end": 1768.97, "word": " Style", "probability": 0.049468994140625}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 1772.982875, "duration_after_vad": 1636.0387499999965}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/YWBUtjE9yHc.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/YWBUtjE9yHc.srt
new file mode 100644
index 0000000000000000000000000000000000000000..2f7bedeac979230ca347002139e6860c44a39655
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/YWBUtjE9yHc.srt
@@ -0,0 +1,1989 @@
+1
+00:00:02,090 --> 00:00:05,750
+السلام عليكم ورحمة الله وبركاته، يعطيكم العافية
+
+2
+00:00:05,750 --> 00:00:09,190
+جميعًا في هذا اللقاء، نستكمل سلسلة الـ biochemical
+
+3
+00:00:09,190 --> 00:00:12,910
+test. في المحاضرة السابقة، تكلمنا على الإنزيمات
+
+4
+00:00:12,910 --> 00:00:16,090
+وقسمنا الإنزيمات حسب الـ site of production إلى
+
+5
+00:00:16,090 --> 00:00:19,930
+endoenzyme و exoenzyme. الـ endoenzyme قلنا أنه
+
+6
+00:00:19,930 --> 00:00:24,670
+التفاعل بيصير intracellular داخل الخلية، لكن الـ
+
+7
+00:00:24,670 --> 00:00:28,590
+exoenzyme التفاعل بيصير extracellular خارج الخلية.
+
+8
+00:00:28,590 --> 00:00:33,770
+في الـ exoenzyme، الإنزيم يفرز داخل الخلية لكن بيطلع
+
+9
+00:00:33,770 --> 00:00:37,150
+برا الخلية لأن الـ substrate اللي بيشتغل عليها الـ
+
+10
+00:00:37,150 --> 00:00:42,670
+exoenzyme حجمها كبير (high molecular weight). يبقى الـ
+
+11
+00:00:42,670 --> 00:00:45,490
+substrate اللي هيشتغل عليها الـ exoenzyme high
+
+12
+00:00:45,490 --> 00:00:49,430
+molecular weight such as polysaccharide, lipid, and
+
+13
+00:00:49,430 --> 00:00:53,850
+protein. هدول يعتبروا macromolecules، ما بيقدروا
+
+14
+00:00:53,850 --> 00:00:57,970
+يدخلوا جوا الخلية، عشان هيك لازم يطلع الإنزيم لبرا
+
+15
+00:00:57,970 --> 00:01:01,870
+ويصير التفاعل extracellular. من الأمثلة على
+
+16
+00:01:01,870 --> 00:01:04,970
+البوليساكرايد الـ starch، من الأمثلة على الليبيد الـ
+
+17
+00:01:04,970 --> 00:01:09,550
+triglyceride، من الأمثلة على الـ protein: fibrinogen,
+
+18
+00:01:09,550 --> 00:01:14,830
+casein, gelatin. الـ amylase إنزيم بيشتغل على الـ
+
+19
+00:01:14,830 --> 00:01:19,070
+starch. الـ lipase بيشتغل على الـ triglyceride. الـ
+
+20
+00:01:19,070 --> 00:01:22,170
+coagulase أخدناها في المحاضرة السابقة، قلنا هو
+
+21
+00:01:22,170 --> 00:01:28,670
+إنزيم بيشتغل على الفيبرنوجين. الكازيناز
+
+22
+00:01:28,670 --> 00:01:32,310
+قلنا بيشتغل على الكازين. الجيلاتيناز بيشتغل على
+
+23
+00:01:32,310 --> 00:01:37,270
+الجيلاتين. هدول كلهم يعتبروا exo-enzyme لأن الـ
+
+24
+00:01:37,270 --> 00:01:42,610
+substrate اللي بيشتغل عليها تعتبر high molecular
+
+25
+00:01:42,610 --> 00:01:46,670
+weight، حجمها كبير، ما بتقدر تدخل لداخل الخلية، عشان
+
+26
+00:01:46,670 --> 00:01:51,990
+هيك الإنزيمات بتفرز داخل الخلية و تطلع لبرا عشان
+
+27
+00:01:51,990 --> 00:01:57,470
+يصير التفاعل extracellular. لو رجعنا لسلايد type
+
+28
+00:01:57,470 --> 00:02:01,790
+of enzyme حسب الـ site of production، في الـ
+
+29
+00:02:01,790 --> 00:02:07,130
+exoenzyme كان ذاكر أمثلة، هنلاقي هذه الإنزيمات هي
+
+30
+00:02:07,130 --> 00:02:13,350
+مذكورة في الجملة. يبقى عشان نحفظ الـ exoenzyme، بشوف
+
+31
+00:02:13,350 --> 00:02:17,590
+شو الـ substrate اللي بيشتغل عليها الـ enzyme. إذا الـ
+
+32
+00:02:17,590 --> 00:02:22,510
+substrate polysaccharide or lipid or protein، يبقى
+
+33
+00:02:22,510 --> 00:02:25,750
+هاي تعتبر high molecular weight، يبقى الـ enzyme
+
+34
+00:02:25,750 --> 00:02:30,160
+يعتبر exoenzyme. اليوم إن شاء الله في هذه المحاضرة
+
+35
+00:02:30,160 --> 00:02:35,580
+هنتكلم عن الأميلاز و الجيلاتيناز. هنبدأ بالأميلاز،
+
+36
+00:02:35,580 --> 00:02:40,340
+production or starch hydrolysis test. أول معلومة
+
+37
+00:02:40,340 --> 00:02:45,600
+عرفناها عن الأميلاز أنه exoenzyme. الـ substrates اللي
+
+38
+00:02:45,600 --> 00:02:49,680
+بيشتغل عليها الـ amylase هي الـ starch. الـ starch
+
+39
+00:02:49,680 --> 00:02:54,940
+يعتبر polysaccharide. الـ amylase بيعمل hydrolysis لـ
+
+40
+00:02:54,940 --> 00:02:59,560
+الـ starch، عشان هي اسم الفحص: starch hydrolysis test
+
+41
+00:02:59,560 --> 00:03:03,540
+or amylase production، لأنه بيعمل hydrolysis للـ
+
+42
+00:03:03,540 --> 00:03:07,600
+starch. الـ starch عبارة عن long chain of
+
+43
+00:03:07,600 --> 00:03:12,150
+monosaccharide. من الـ monosaccharide الجلوكوز. لو
+
+44
+00:03:12,150 --> 00:03:16,730
+بدنا نتعرف على الـ type of sugar، الـ types of
+
+45
+00:03:16,730 --> 00:03:20,410
+sugar، فيه عندي monosaccharide, disaccharide، و
+
+46
+00:03:20,410 --> 00:03:25,370
+polysaccharide. الـ monosaccharide: جلوكوز، galactose،
+
+47
+00:03:25,370 --> 00:03:29,050
+فركتوز، هي أمثلة على monosaccharide. الـ
+
+48
+00:03:29,050 --> 00:03:33,150
+disaccharide بتتكون من two monosaccharide مرتبطين.
+
+49
+00:03:33,150 --> 00:03:37,900
+من الأمثلة على الـ disaccharide: sucrose,
+
+50
+00:03:38,020 --> 00:03:41,680
+maltose, lactose. الـ sucrose عبارة عن جلوكوز
+
+51
+00:03:41,680 --> 00:03:46,320
+و فركتوز. المالتوز عبارة عن جلوكوز زائد جلوكوز.
+
+52
+00:03:46,320 --> 00:03:51,200
+اللاكتوز: جلوكوز زائد جلاكتوز. ثالث نوع: الـ
+
+53
+00:03:51,200 --> 00:03:54,720
+polysaccharide، مثال عليها الـ starch. قلنا أن الـ
+
+54
+00:03:54,720 --> 00:03:59,560
+starch عبارة عن polymer of monosaccharide، من الـ mono
+
+55
+00:03:59,560 --> 00:04:03,080
+saccharide الجلوكوز. من الأمثلة الأخرى على الـ
+
+56
+00:04:03,080 --> 00:04:06,620
+polysaccharide، في عندي الجليكوجين وعندي السليلوز.
+
+57
+00:04:07,200 --> 00:04:11,360
+لكن اليوم إن شاء الله هنتكلم إحنا عن الـ amylase
+
+58
+00:04:11,360 --> 00:04:15,420
+اللي بيشتغل على الـ starch. الـ starch يعتبر
+
+59
+00:04:15,420 --> 00:04:20,140
+polysaccharide، بتكون من polymer of glucose. الـ
+
+60
+00:04:20,140 --> 00:04:24,380
+starch hydrolysis، تكسير الـ starch بيصير بواسطة
+
+61
+00:04:24,380 --> 00:04:29,400
+two types of enzyme، بواسطة نوعين من الإنزيمات. أول
+
+62
+00:04:29,400 --> 00:04:35,060
+إنزيم: alpha amylase (alpha وليس beta لأن الـ beta لها
+
+63
+00:04:35,060 --> 00:04:38,420
+علاقة بالـ cellulose). الـ cellulose الموجود في
+
+64
+00:04:38,420 --> 00:04:44,980
+النبات. الـ alpha amylase بيعمل hydrolysis of α1,4-
+
+65
+00:04:44,980 --> 00:04:49,880
+glycosidic bond، بيكسر الرابطة اللي اسمها α1,4-
+
+66
+00:04:49,880 --> 00:04:54,520
+glycosidic bond. الإنزيم الثاني المسؤول عن تكسير
+
+67
+00:04:54,520 --> 00:04:59,880
+الـ starch: oligo-1,6-glycosidase، مسؤول عن تكسير
+
+68
+00:04:59,880 --> 00:05:05,920
+الرابطة α1,6-glycosidic bond. عشان نوضح الصورة أكثر،
+
+69
+00:05:05,920 --> 00:05:09,000
+هذه
+
+70
+00:05:09,000 --> 00:05:14,820
+الصورة بتوضح الـ starch structure. الـ starch قلنا
+
+71
+00:05:14,820 --> 00:05:18,240
+عبارة عن long chain of monosaccharide. الـ
+
+72
+00:05:18,240 --> 00:05:21,360
+monosaccharide اللي هو الجلوكوز، يبقى هو عبارة عن
+
+73
+00:05:21,360 --> 00:05:25,760
+سلسلة من الجلوكوز. هاي الوحدات الخضراء عبارة عن
+
+74
+00:05:25,760 --> 00:05:32,640
+الجلوكوز. إذا كانت الـ structure chain، الرابطة ما
+
+75
+00:05:32,640 --> 00:05:36,900
+بينهم بتكون alpha 1-4 linkage. لو كان في عندي
+
+76
+00:05:36,900 --> 00:05:41,620
+branch، بتكون الرابطة alpha 1-6 linkage. إحنا
+
+77
+00:05:41,620 --> 00:05:46,900
+قلنا أنه في عندي two types of enzyme، مسئولين عن الـ
+
+78
+00:05:46,900 --> 00:05:51,020
+starch hydrolysis. الإنزيم الأول اللي هو الـ alpha
+
+79
+00:05:51,020 --> 00:05:54,720
+amylase. الـ alpha amylase قلنا مسؤول عن تكسير الـ
+
+80
+00:05:55,030 --> 00:05:59,410
+الرابطة، hydrolysis للرابطة alpha 1-4 في
+
+81
+00:05:59,410 --> 00:06:03,550
+glycosidic bond. يبقى الـ alpha amylase هيجي هنا في
+
+82
+00:06:03,550 --> 00:06:08,030
+حال كان في عندي chain، في عندي سلسلة، هيجي يكسر هاي
+
+83
+00:06:08,030 --> 00:06:12,950
+الروابط، ويعطيني:  لو كسر هنا، لو إيجي هنا من هاي
+
+84
+00:06:12,950 --> 00:06:17,530
+الرابطة، لو
+
+85
+00:06:17,530 --> 00:06:23,430
+كسر هنا إثنين جلوكوز، أداني مالتوز. لو كسر هنا، هيديني
+
+86
+00:06:23,430 --> 00:06:29,950
+ثلاثة جلوكوز، بسميهم maltotriose. تمام. لو اتكسرت
+
+87
+00:06:29,950 --> 00:06:33,630
+هنا الرابطة هذا، المركب إذا ظل الـ branch، بسميه
+
+88
+00:06:33,630 --> 00:06:39,030
+dextrin. هيجي الـ alpha أو oligo 1-6
+
+89
+00:06:39,030 --> 00:06:43,670
+glycosidase، هيكسر الرابطة اللي هنا اللي هي alpha 1-6
+
+90
+00:06:43,670 --> 00:06:49,170
+linkage، ويديني maltose و maltotriose. هذه كلها
+
+91
+00:06:49,170 --> 00:06:55,120
+المركبات عبارة عن low molecular weight، soluble in
+
+92
+00:06:55,120 --> 00:06:58,980
+water، بتقدر تدخل للخلية عبر الـ cell membrane،
+
+93
+00:06:58,980 --> 00:07:03,600
+تستخدم في الـ energy production خلال عملية الـ
+
+94
+00:07:03,600 --> 00:07:08,140
+glycolysis. كمان تستخدم كـ carbon source. الـ carbon
+
+95
+00:07:08,140 --> 00:07:11,580
+source طبعًا عندي أنا الـ carbohydrate هي من
+
+96
+00:07:11,580 --> 00:07:18,060
+الفوائدها اللي هي تعتبر كمصدر للكربون اللازم لنمو
+
+97
+00:07:18,060 --> 00:07:21,530
+البكتيريا. يبقى هاي كلها تعتبر low molecular
+
+98
+00:07:21,530 --> 00:07:26,450
+weight، بتقدر تدخل للخلية، الخلية بتقدر تستفيد منها
+
+99
+00:07:26,450 --> 00:07:31,470
+ممكن كمصدر للطاقة، أو ممكن كمصدر للكربون اللازم
+
+100
+00:07:31,470 --> 00:07:39,070
+لنمو البكتيريا. نرجع
+
+101
+00:07:39,070 --> 00:07:45,020
+لأول slide. Starch agar is a differential medium. لما
+
+102
+00:07:45,020 --> 00:07:49,980
+قسمنا الـ media حسب الـ function، قلنا أنه في عندي
+
+103
+00:07:49,980 --> 00:07:53,460
+differential media. الـ differential media بتعطي كل
+
+104
+00:07:53,460 --> 00:07:58,180
+بكتيريا لون، بالتالي بتميز بكتيريا عن بكتيريا، وهذا
+
+105
+00:07:58,180 --> 00:08:01,940
+إن شاء الله هنشوفه في الـ result. That test the
+
+106
+00:08:01,940 --> 00:08:05,140
+ability of an organism to produce certain
+
+107
+00:08:05,140 --> 00:08:09,640
+exoenzymes. ولهذا الفحص بيكشف عن قدرة الـ organism
+
+108
+00:08:10,200 --> 00:08:14,500
+أنه ينتج certain exoenzymes. قلنا إن الـ amylase
+
+109
+00:08:14,500 --> 00:08:19,220
+يعتبر exoenzyme. الإنزيمات المسؤولة عن الـ starch
+
+110
+00:08:19,220 --> 00:08:23,020
+hydrolysis: أول إنزيم اللي هو الـ alpha amylase،
+
+111
+00:08:23,020 --> 00:08:27,120
+المسؤول عن تكسير الرابطة alpha-1,4-glycosidic
+
+112
+00:08:27,120 --> 00:08:30,840
+bond. الإنزيم الثاني، يبقى هذا أول إنزيم اللي هو الـ
+
+113
+00:08:30,840 --> 00:08:31,840
+alpha amylase.
+
+114
+00:08:35,090 --> 00:08:39,810
+الأنزيم الثاني اللي هو oligo-1,6-glycosidase،
+
+115
+00:08:39,810 --> 00:08:45,570
+المسؤول عن تكسير الرابطة alpha-1,6-glycosidic bond،
+
+116
+00:08:45,570 --> 00:08:50,790
+that hydrolyze starch، بيكسروا الـ starch لمركبات
+
+117
+00:08:50,790 --> 00:08:56,130
+صغيرة الحجم بتقدر تدخل للخلية. Starch molecules are
+
+118
+00:08:56,130 --> 00:08:59,490
+too large to enter the bacterial cell. قلنا إن الـ
+
+119
+00:08:59,490 --> 00:09:03,670
+starch يعتبر polysaccharide، حجمه كبير، ما بيقدر
+
+120
+00:09:03,670 --> 00:09:08,710
+يدخل الخلية. So some bacteria, some، مش كل البكتيريا
+
+121
+00:09:08,710 --> 00:09:13,410
+بتفرز الإنزيمات المسؤولة عن تكسير الـ starch. Some
+
+122
+00:09:13,410 --> 00:09:18,110
+bacteria secrete exoenzymes. اتفقنا أنه عبارة عن
+
+123
+00:09:18,110 --> 00:09:22,550
+exoenzyme، to degrade starch into subunits، بيكسر الـ
+
+124
+00:09:22,550 --> 00:09:25,870
+starch لـ subunits، زي ما اتعرفنا: مالتوز، مالتو
+
+125
+00:09:25,870 --> 00:09:30,550
+تريوز، أو دكسترين. هاي الـ subunits that can then be
+
+126
+00:09:30,550 --> 00:09:33,810
+utilized by the organism، بتقدر تدخل للخلية،
+
+127
+00:09:33,810 --> 00:09:43,090
+والـ organism يقدر أنه يستهلكها ويستفيد منها. هيك
+
+128
+00:09:43,090 --> 00:09:48,930
+إحنا عرفنا كيف الإنزيم بيكسر الـ starch، كيف شغل
+
+129
+00:09:48,930 --> 00:09:52,930
+الأنزيم. طب كيف بدنا نكشف عن هذا الإنزيم، وهل
+
+130
+00:09:52,930 --> 00:09:59,270
+البكتيريا قادرة إنها تكسر الـ starch أو لأ؟ إحنا قلنا
+
+131
+00:09:59,270 --> 00:10:04,190
+إن أي إنزيم بيشتغل على substrates، الأميلاز بيشتغل
+
+132
+00:10:04,190 --> 00:10:09,170
+على الـ starch، يبقى عشان أكشف على الأميلاز، لازم
+
+133
+00:10:09,170 --> 00:10:14,670
+أزرع البكتيريا على ميديا فيها starch: starch agar
+
+134
+00:10:14,670 --> 00:10:20,350
+is a simple nutritive media. بقول هي عبارة عن media
+
+135
+00:10:20,350 --> 00:10:24,830
+simple with starch added. يبقى هي عبارة عن nutrient
+
+136
+00:10:24,830 --> 00:10:29,730
+agar مضاف إليها الـ starch as a supplement. السترش هو
+
+137
+00:10:29,730 --> 00:10:32,890
+عبارة عن الـ substrate اللي هيشتغل عليه الإنزيم،
+
+138
+00:10:32,890 --> 00:10:36,930
+فبالتالي لازم يكون موجود في الميديا. السترش أجار
+
+139
+00:10:36,930 --> 00:10:41,490
+ممكن تيجي جاهزة ببوتل، وممكن أنا أحضرها. كيف بحضرها؟
+
+140
+00:10:41,490 --> 00:10:47,170
+بجيب الـ nutrient agar و بضيف عليها starch. Since no
+
+141
+00:10:47,170 --> 00:10:50,210
+color change occurs in the media when organisms
+
+142
+00:10:50,210 --> 00:10:54,550
+hydrolyze starch, we added, we add iodine to the
+
+143
+00:10:54,550 --> 00:10:58,950
+plate after incubation. Iodine turns blue, purple, or
+
+144
+00:10:58,950 --> 00:11:02,370
+black, dependent on the concentration of iodine in
+
+145
+00:11:02,370 --> 00:11:06,370
+the presence of starch. عشان نفهم شو قصده بهذه
+
+146
+00:11:06,370 --> 00:11:11,110
+الفقرة، خلينا نشرح الـ procedure. هذه الفقرة هي الـ
+
+147
+00:11:11,110 --> 00:11:15,990
+principle of the test. من هنا هي الـ principle of the test، لكن
+
+148
+00:11:15,990 --> 00:11:18,470
+خلينا نحكي عن الـ procedure.
+
+149
+00:11:26,190 --> 00:11:30,630
+الـ procedure بقول لي إنه أنا بحضر الـ starch agar، طبعًا
+
+150
+00:11:30,630 --> 00:11:34,470
+زي خطوات تحضير الـ media اللي أخذناها. بعد ما أحضر
+
+151
+00:11:34,470 --> 00:11:38,310
+الـ starch agar، بزرع البكتيريا اللي بدي أفحص قدرتها
+
+152
+00:11:38,310 --> 00:11:42,590
+على إنتاج هذا الإنزيم. كيف طريقة الزراعة؟ بزرع على
+
+153
+00:11:42,590 --> 00:11:47,170
+شكل خط. يبقى بقول لي: inoculate the test organism across
+
+154
+00:11:47,170 --> 00:11:53,020
+a sample portion of the agar surface. أمن الزراعة الـ
+
+155
+00:11:53,020 --> 00:11:56,000
+organism اللي أنا بدي أفحص قدرته على إنتاج هذا
+
+156
+00:11:56,000 --> 00:12:01,440
+الأنزيم بشكل خط. باخد الطبق بعد ما أنا زرعت
+
+157
+00:12:01,440 --> 00:12:06,320
+طبعًا، بحط هذا الطبق اللي أنا زرعته في الـ incubator
+
+158
+00:12:06,320 --> 00:12:14,020
+على درجة حرارة 37 لمدة 48 ساعة أو 24. بعد الـ
+
+159
+00:12:14,020 --> 00:12:19,480
+incubation، طبعًا هلاقي أنا عندي نمو للبكتيريا. زرعت
+
+160
+00:12:19,480 --> 00:12:23,460
+البكتيريا، نمت البكتيريا. إذا كان عندها إنزيم
+
+161
+00:12:23,460 --> 00:12:27,180
+الاميلاز، هتكون كسرت الـ starch. وإذا ما كان عندها
+
+162
+00:12:27,180 --> 00:12:31,640
+الأنزيم، مش هتكون كسرت الـ starch. طيب كيف بدي أكشف
+
+163
+00:12:31,640 --> 00:12:37,340
+عن هالشيء؟ بروح أنا بضيف indicator، أو مادة تتفاعل
+
+164
+00:12:37,340 --> 00:12:41,980
+مع الـ starch، وتعطي لون معين. لو البكتيريا عندها
+
+165
+00:12:41,980 --> 00:12:46,420
+إنزيم الأميلاز، هتكسر الـ starch، يعني الـ starch مش
+
+166
+00:12:46,420 --> 00:12:51,240
+موجود، يعني مش هيصير تفاعل، وما رح يعطي اللون المعين.
+
+167
+00:12:51,890 --> 00:12:56,770
+لكن لو البكتيريا ما عندها الـ enzyme، الـ starch ما
+
+168
+00:12:56,770 --> 00:13:01,050
+رح يتكسر، هيظل موجود في الـ media، يعني هيصير التفاعل
+
+169
+00:13:01,050 --> 00:13:06,210
+يعطي لون معين. هذا هو الـ principle of the test. طيب شو هو
+
+170
+00:13:06,210 --> 00:13:10,510
+الـ indicator اللي إحنا بنضيفه؟ بقول لك بتضيف iodine.
+
+171
+00:13:10,510 --> 00:13:14,470
+الـ iodine، أخذناها في الـ gram stain، نفسه اللي إحنا
+
+172
+00:13:14,470 --> 00:13:18,880
+استخدمناه في الـ gram stain. بقول لك بعد الـ
+
+173
+00:13:18,880 --> 00:13:22,980
+incubation لمدة 24 ساعة، وبعد ما تنمو البكتيريا،
+
+174
+00:13:22,980 --> 00:13:28,540
+بغمر الطبق بالـ iodine، بغمر كل الطبق بالـ iodine،
+
+1
+
+223
+00:17:03,300 --> 00:17:07,200
+مثال عليها الـ E. coli هذه الصورة هي في عندي clear
+
+224
+00:17:07,200 --> 00:17:11,920
+zone حوالين النمو يبقى هي النتيجة positive  هاي بصيلة
+
+225
+00:17:11,920 --> 00:17:16,720
+لـ subcules لكن هنا بلاحظ أن في عندي اللون أسود
+
+226
+00:17:16,720 --> 00:17:22,280
+حوالين البكتيريا أو حوالين النمو يبقى هي الـ E.coli
+
+227
+00:17:22,280 --> 00:17:27,700
+طبعاً بلاحظ هنا أن هاي المناطق ما وصلت إليها
+
+228
+00:17:27,700 --> 00:17:32,780
+البكتيريا فبالتالي سيظل الـ starch موجود سيتّرتبط بالـ
+
+229
+00:17:32,780 --> 00:17:34,680
+اليود ويعطيني اللون الأسود
+
+230
+00:17:38,890 --> 00:17:43,370
+في الـ slide اللي قبل كان ذاكر أن البصيلة الـ sub
+
+231
+00:17:43,370 --> 00:17:47,230
+-cultures البصيلة
+
+232
+00:17:47,230 --> 00:17:50,470
+الـ sub-cultures هي positive للبحث والفحص والـ
+
+233
+00:17:50,470 --> 00:17:54,990
+E.coli negative للفحص لكن ما بحكي أن هذا يعتبر
+
+234
+00:17:54,990 --> 00:17:59,460
+significant، هذا ليس significant فقط، لكن مثال على الـ
+
+235
+00:17:59,460 --> 00:18:02,740
+positive والـ negative للفحص لأن الـ E.coli جرام
+
+236
+00:18:02,740 --> 00:18:06,100
+نيجاتيف، بصيلاية، والبصيلة sub-cultures تعتبر جرام
+
+237
+00:18:06,100 --> 00:18:09,480
+positive بصيلاية، يبقى من الأصل من الجرام-ستينين بقدر
+
+238
+00:18:09,480 --> 00:18:13,500
+أني أنا أفرّق بينهم، غير كده أن الـ E.coli تعتبر non
+
+239
+00:18:13,500 --> 00:18:17,360
+spore forming، البصيلة sub-cultures تعتبر spore forming
+
+240
+00:18:17,360 --> 00:18:22,600
+لو بدنا نفرّق، الأميليز positive للبصيلة sub-cultures
+
+241
+00:18:22,600 --> 00:18:28,010
+والـ E.coli اللي هي الأميليز negative، هكذا نكون قد أنهينا
+
+242
+00:18:28,010 --> 00:18:33,570
+أول فحص اللي هو الأميليز، سنتقل للجيلاتيناز
+
+243
+00:18:33,570 --> 00:18:40,910
+سنتكلم
+
+244
+00:18:40,910 --> 00:18:44,830
+عن ثاني إنزيم اللي هو الجيلاتيناز، الجيلاتيناز
+
+245
+00:18:44,830 --> 00:18:48,990
+يعتبر exo enzyme، الـ substrate اللي سيعمل عليها
+
+246
+00:18:48,990 --> 00:18:53,920
+الجيلاتين، قلنا أن الجيلاتين يعتبر protein high
+
+247
+00:18:53,920 --> 00:18:58,600
+molecular weight، لازم الإنزيم يخرج ويُكسره إلى
+
+248
+00:18:58,600 --> 00:19:03,320
+low molecular weight، سيكسره إلى amino acid، الـ amino
+
+249
+00:19:03,320 --> 00:19:06,740
+acid هي سوليوبل، تستطيع الدخول إلى الـ cell membrane
+
+250
+00:19:07,210 --> 00:19:12,050
+الأمينو أسيد تعتبر كمصدر للنيتروجين، نيتروجين source
+
+251
+00:19:12,050 --> 00:19:15,790
+تستخدم في الـ synthesis of structural and
+
+252
+00:19:15,790 --> 00:19:20,950
+functional cellular protein اللازم لنمو البكتيريا
+
+253
+00:19:20,950 --> 00:19:24,050
+الجيلاتين
+
+254
+00:19:24,050 --> 00:19:28,550
+يعتبر بروتين مشتق من الكولاجين الموجود في الـ
+
+255
+00:19:28,550 --> 00:19:34,010
+connective tissue، كانوا زمان يعتبرون الجيلاتين كـ
+
+256
+00:19:34,010 --> 00:19:38,590
+solidifying agent، لكن في أنواع من البكتيريا تُفرز
+
+257
+00:19:38,590 --> 00:19:43,450
+إنزيم الجيلاتيناز، يكسر الجيلاتين، ويُحوّل الوسط من
+
+258
+00:19:43,450 --> 00:19:49,630
+solid إلى liquid، وهذه هي فكرة الكشف عن الإنزيم، ومعرفة هل
+
+259
+00:19:49,630 --> 00:19:54,210
+البكتيريا عندها إنزيم الجيلاتيناز أم لا، الفكرة أنه
+
+260
+00:19:54,210 --> 00:19:59,010
+يكون عندي في الـ medium جيلاتين كـ substrate
+
+261
+00:19:59,710 --> 00:20:03,590
+الجيلاتيناز طبعاً في البكتيريا، سأكشف هل البكتيريا
+
+262
+00:20:03,590 --> 00:20:07,290
+عندها هذا الإنزيم أم لا، هو exo enzyme، لو
+
+263
+00:20:07,290 --> 00:20:11,290
+البكتيريا عندها إنزيم الجيلاتيناز، ستُكسّر الجيلاتين،
+
+264
+00:20:11,290 --> 00:20:15,670
+ستُحوّله إلى amino acid زائد small peptide، يبقى
+
+265
+00:20:15,670 --> 00:20:19,710
+الجيلاتين كان هو يعتبر solidifying agent، هو الذي
+
+266
+00:20:19,710 --> 00:20:23,910
+جعل الـ media صلبة، لما تكسّر الجيلاتين صار الوسط
+
+267
+00:20:23,910 --> 00:20:28,730
+سائلاً liquid، يبقى هذا هو الـ principle، الفحص، إنّه الـ
+
+268
+00:20:28,730 --> 00:20:38,430
+media بعد ما كانت صلبة ستصبح سائلة، Determine
+
+269
+00:20:38,430 --> 00:20:42,430
+an organism's ability to produce proteolytic-like
+
+270
+00:20:42,430 --> 00:20:46,510
+enzyme and lycophygelatin، سأقول لك هذا الفحص لكي
+
+271
+00:20:46,510 --> 00:20:51,510
+أعرف هل البكتيريا تُفرز إنزيمات تعمل البروتيوليتك،
+
+272
+00:20:51,510 --> 00:20:56,290
+تحلل البروتينات، وتجعل الوسط سائلاً، البروتيوليتك
+
+273
+00:20:56,290 --> 00:21:00,430
+يعني license of a protein، this test is used to
+
+274
+00:21:00,430 --> 00:21:05,210
+differentiate gram negative species، هذه الفقرة هي
+
+275
+00:21:05,210 --> 00:21:08,850
+الـ significant، قلنا كل فحص له  significant
+
+276
+00:21:08,850 --> 00:21:13,050
+من الـ positive والـ negative، أو يُفرّق بين من ومَن،
+
+277
+00:21:13,050 --> 00:21:16,730
+ولها هذا، يُستخدم to differentiate gram negative
+
+278
+00:21:16,730 --> 00:21:23,770
+species: Serratia، Pseudomonas، وVibrio، هؤلاء جميعهم
+
+279
+00:21:23,770 --> 00:21:27,590
+positive لفحص الجيلاتين، عندهم جيلاتيناز يُكسّر
+
+280
+00:21:27,590 --> 00:21:31,150
+الجيلاتين، الـ test can be used to differentiate
+
+281
+00:21:31,150 --> 00:21:36,390
+genera of gelatinase producing bacteria such as
+
+282
+00:21:36,390 --> 00:21:40,290
+Serratia and Proteus from other members of the
+
+283
+00:21:40,290 --> 00:21:45,090
+family Enterobacteriaceae، أقول لك أن هذا الفحص
+
+284
+00:21:45,090 --> 00:21:49,720
+يُميّز الـ Proteus والـ Serratia، هؤلاء يعتبروا positive
+
+285
+00:21:49,720 --> 00:21:54,180
+للجيلاتيناز، عن باقي أعضاء الـ Enterobacteriaceae
+
+286
+00:21:54,180 --> 00:21:58,360
+الـ Enterobacteriaceae عائلة تضم أكثر من بكتيريا من
+
+287
+00:21:58,360 --> 00:22:03,280
+ضمنها الـ Serratia والـ Proteus، هؤلاء هما فقط الـ
+
+288
+00:22:03,280 --> 00:22:06,680
+positive لهذا الفحص من عائلة الـ Enterobacteriaceae
+
+289
+00:22:06,680 --> 00:22:11,280
+فبالتالي، من الممكن أن أفرّق هؤلاء عن باقي أعضاء
+
+290
+00:22:11,280 --> 00:22:16,890
+الـ Enterobacteriaceae بفحص الجيلاتيناز، أقول لي أن الفحص
+
+291
+00:22:16,890 --> 00:22:20,710
+هذا هو الـ issue العملي لهذا الفحص، إلى الآن لم يُقدر
+
+292
+00:22:20,710 --> 00:22:26,310
+وفي طرق للكشف عن الإنزيم بشكل أسرع، سنتعرّف عليها
+
+293
+00:22:26,310 --> 00:22:31,370
+خلال المحاضرة، Staphylococcus aureus which is
+
+294
+00:22:31,370 --> 00:22:34,750
+gelatinase positive، can be differentiated from
+
+295
+00:22:34,750 --> 00:22:38,490
+Staphylococcus epidermidis gelatinase negative
+
+296
+00:22:39,500 --> 00:22:43,460
+الـ Staphylococcus aureus جيلاتيناز positive
+
+297
+00:22:43,460 --> 00:22:47,240
+لكن الـ Staphylococcus epidermidis تعتبر جيلاتيناز
+
+298
+00:22:47,240 --> 00:22:52,140
+negative، قلنا أن الـ Staphylococcus بشكل عام
+
+299
+00:22:52,140 --> 00:22:58,260
+الـ genus هي gram positive cocci، catalase positive، تمام
+
+300
+00:22:58,570 --> 00:23:02,350
+الـ Staphylococci catalase positive، لعشان أفرّق الـ species
+
+301
+00:23:02,350 --> 00:23:07,190
+عن بعض، ذكرنا فحص كيميائي اللي هو الـ coagulase
+
+302
+00:23:07,190 --> 00:23:11,110
+قلنا أن الـ Staphylococcus aureus coagulase positive
+
+303
+00:23:11,110 --> 00:23:14,490
+لكن الـ Staphylococcus epidermidis coagulase negative
+
+304
+00:23:14,490 --> 00:23:18,230
+فحص ثاني اللي هو الجيلاتيناز، نفس الشيء
+
+305
+00:23:18,230 --> 00:23:22,430
+الـ Staphylococcus aureus جيلاتيناز positive لكن
+
+306
+00:23:22,430 --> 00:23:26,610
+الـ Staphylococcus epidermidis جيلاتيناز negative، الـ
+
+307
+00:23:26,610 --> 00:23:29,930
+Staphylococcus aureus قلنا هي pathogenic لكن الـ
+
+308
+00:23:29,930 --> 00:23:33,550
+Staphylococcus epidermidis هي opportunistic في
+
+309
+00:23:33,550 --> 00:23:39,670
+كمان فحصين سيمران معنا خلال الشرائح، هذا هو المقارنة
+
+310
+00:23:39,670 --> 00:23:42,890
+بينهما، gram positive cocci، catalase positive لـ
+
+311
+00:23:42,890 --> 00:23:45,650
+Staphylococcus aureus لكن في بعض species لـ
+
+312
+00:23:45,650 --> 00:23:47,850
+Staphylococcus aureus ولـ Staphylococcus
+
+313
+00:23:47,850 --> 00:23:50,590
+epidermidis، قلنا الـ coagulase positive لـ
+
+314
+00:23:50,590 --> 00:23:54,050
+Staphylococcus aureus والجيلاتيناز positive، في
+
+315
+00:23:54,050 --> 00:23:57,190
+عندي فحصين سنتعرف عليهما أو سيمران معنا خلال
+
+316
+00:23:57,190 --> 00:24:01,150
+الشباتر القادمة، الـ mannitol والـ DNAse أيضاً positive
+
+317
+00:24:01,150 --> 00:24:04,370
+في الـ Staphylococcus aureus، لكن negative في
+
+318
+00:24:04,370 --> 00:24:06,190
+الـ Staphylococcus epidermidis
+
+319
+00:24:09,210 --> 00:24:12,990
+Bacillus anthracis and Bacillus cereus and several
+
+320
+00:24:12,990 --> 00:24:17,570
+other members of the genus are gelatinase positive
+
+321
+00:24:17,570 --> 00:24:21,250
+as are Clostridium tetani and Clostridium
+
+322
+00:24:21,250 --> 00:24:24,750
+perfringens، قلنا أن الـ Clostridium والـ Bacillus
+
+323
+00:24:24,750 --> 00:24:29,570
+يعتبروا gram positive bacilli، spore forming، نفرّق
+
+324
+00:24:29,570 --> 00:24:34,120
+بينهم بفحص الكاتلاز، البصيلة الـ cereus والبصيلة
+
+325
+00:24:34,120 --> 00:24:38,740
+الـ anthracis أقول عنهما جيلاتيناز positive، وكما الـ tetani
+
+326
+00:24:38,740 --> 00:24:43,420
+إذا أردنا ربط المعلومات، كانوا يعتبرون central in
+
+327
+00:24:43,420 --> 00:24:47,500
+the endospore، الـ Clostridium tetani والـ Clostridium
+
+328
+00:24:47,500 --> 00:24:51,420
+perfringens أيضاً يعتبروا جيلاتيناز positive، يبقى هذا
+
+329
+00:24:51,420 --> 00:24:54,440
+هو الـ significant للفحص
+
+330
+00:24:57,230 --> 00:25:01,310
+Gelatin is a protein، أقول لك هو عبارة عن بروتين
+
+331
+00:25:01,310 --> 00:25:06,270
+derived from collagen، هو عبارة عن بروتين مشتق من
+
+332
+00:25:06,270 --> 00:25:09,610
+الـ collagen الموجود في الـ connective tissue
+
+333
+00:25:09,610 --> 00:25:14,090
+Gelatinase comprises a family of extracellular
+
+334
+00:25:14,090 --> 00:25:18,070
+enzymes، قلنا الجيلاتيناز يعتبر من الـ exoenzymes
+
+335
+00:25:18,070 --> 00:25:22,610
+produced and secreted by some microorganisms، قلنا
+
+336
+00:25:22,610 --> 00:25:26,760
+يتم إنتاجه بواسطة بعض أنواع البكتيريا، ليس كل
+
+337
+00:25:26,760 --> 00:25:30,540
+البكتيريا عندها إنزيم الجيلاتيناز، to hydrolyze
+
+338
+00:25:30,540 --> 00:25:34,800
+الجيلاتين، لكي يُكسّر الجيلاتين ويتحوّل إلى amino acid
+
+339
+00:25:34,800 --> 00:25:39,340
+هذا الـ amino acid يعتبر nitrogen source، يُستخدم في
+
+340
+00:25:39,340 --> 00:25:44,340
+إنتاج البروتين، Gelatin is a simple protein، اتفقنا
+
+341
+00:25:44,340 --> 00:25:48,680
+هو عبارة عن simple protein، هذه المعلومة مهمة جداً
+
+342
+00:25:48,680 --> 00:25:52,460
+أقول لك أن الجيلاتين يكون solid عندما تكون درجة
+
+343
+00:25:52,460 --> 00:25:58,220
+الحرارة 25 درجة سليزيوسية أو أقل، يكون
+
+344
+00:25:58,220 --> 00:26:01,700
+liquid عندما تكون درجة الحرارة 26 درجة
+
+345
+00:26:01,700 --> 00:26:07,020
+سليزيوس أو أعلى، يبقى الجيلاتين تتغيّر حالته حسب
+
+346
+00:26:07,020 --> 00:26:11,380
+درجة الحرارة، وهذه معلومة مهمة جداً، والـ bacteria
+
+347
+00:26:11,380 --> 00:26:15,060
+that produce the enzyme gelatinase are grown in a
+
+348
+00:26:15,060 --> 00:26:19,570
+gelatin medium، the enzyme breaks up the gelatin
+
+349
+00:26:19,570 --> 00:26:23,390
+molecule and the medium cannot solidify even at
+
+350
+00:26:23,390 --> 00:26:27,590
+cold temperature، هذه الجملة أو الفقرة هي الـ
+
+351
+00:26:27,590 --> 00:26:31,050
+principle للفحص، أقول لك لو نمّيت البكتيريا التي
+
+352
+00:26:31,050 --> 00:26:35,770
+عندها الـ exoenzyme الجيلاتيناز في وسط فيه جيلاتين
+
+353
+00:26:35,770 --> 00:26:39,800
+الذي هو الـ substrate، أكيد الإنزيم الجيلاتيناز
+
+354
+00:26:39,800 --> 00:26:43,780
+الموجود في البكتيريا سيعمل على الـ substrate، الـ
+
+355
+00:26:43,780 --> 00:26:46,960
+substrate الذي هو الجيلاتين الموجود في الـ media
+
+356
+00:26:46,960 --> 00:26:51,400
+سيكسر الجيلاتين الذي هو solid، سيُحوّله إلى amino acid
+
+357
+00:26:51,400 --> 00:26:56,440
+زائد small peptide، سيبطل الوسط صلب، سيصبح الوسط
+
+358
+00:26:56,440 --> 00:27:00,320
+سائلاً، يبقى الـ media لن تستطيع التصلّب حتى في الـ cold
+
+359
+00:27:00,320 --> 00:27:04,360
+temperature، طيب لماذا قال لي حتى في الـ cold
+
+360
+00:27:04,360 --> 00:27:04,980
+temperature؟
+
+361
+00:27:07,390 --> 00:27:11,090
+لأنّه في الجملة التي قبلها قال لنا أن الجيلاتين يكون
+
+362
+00:27:11,090 --> 00:27:16,630
+صلباً في درجات الحرارة المنخفضة، عند 25 درجة سليزيوسية
+
+363
+00:27:16,630 --> 00:27:21,650
+أو أقل، يعني يكون صلبًا في درجات الحرارة المنخفضة، طيب بما
+
+364
+00:27:21,650 --> 00:27:25,330
+أنه تكسّر الجيلاتين، لم يعد هناك جيلاتين، أصبح amino
+
+365
+00:27:25,330 --> 00:27:29,730
+acid، يبقى لن يتصلّب الـ media حتى لو كانت درجة
+
+366
+00:27:29,730 --> 00:27:33,770
+الحرارة منخفضة، two methods used to determine
+
+367
+00:27:33,770 --> 00:27:37,940
+gelatinase production، أقول لك فيه طريقتان لكشف
+
+368
+00:27:37,940 --> 00:27:41,640
+على إنتاج الجيلاتيناز، الطريقة الأولى gelatin
+
+369
+00:27:41,640 --> 00:27:45,540
+stab method، الطريقة الثانية gelatin strip method
+
+370
+00:27:45,540 --> 00:27:53,860
+سنضيف طريقة ثالثة وهي le charcoal، طريقة
+
+371
+00:27:53,860 --> 00:27:58,060
+ثالثة وهي اسمها le charcoal method
+
+372
+00:28:10,660 --> 00:28:19,700
+الـ charcoal method، يبقى
+
+373
+00:28:19,700 --> 00:28:22,440
+الطريقة الأولى gelatin stab method، الجيلاتين
+
+374
+00:28:22,440 --> 00:28:26,220
+strip method، الـ charcoal method، سنتعرّف على كل طريقة
+
+375
+00:28:26,220 --> 00:28:30,040
+ما هو الـ principle لها، ما هي طريقة الشغل، وكيف تكون
+
+376
+00:28:30,040 --> 00:28:30,580
+الـ result؟
+
+377
+00:28:35,040 --> 00:28:38,640
+في هذه الـ slide ذاكر Serratia، Pseudomonas، Vibrio
+
+378
+00:28:38,640 --> 00:28:45,120
+جميعهم يعتبروا جيلاتيناز positive، أول طريقة لعشان
+
+379
+00:28:45,120 --> 00:28:48,960
+أكشف على الجيلاتيناز هي الـ gelatin stab method
+
+380
+00:28:48,960 --> 00:28:54,740
+الطريقة هي أني أنا آخذ التيوب، طبعاً مكتوب عندي أنا
+
+381
+00:28:54,740 --> 00:28:59,540
+أستعملها، سأعمل stabbing، طعن، ستكون في التيوب
+
+382
+00:28:59,710 --> 00:29:04,870
+nutrient broth، يحتوي على nutrient broth، وليس
+
+383
+00:29:04,870 --> 00:29:09,990
+أجار، ضروري جداً أن يكون broth وليس أجار لأن الأجار
+
+384
+00:29:09,990 --> 00:29:14,250
+يعتبر solidifying agent، ستظل صلبة سواء كانت
+
+385
+00:29:14,250 --> 00:29:18,670
+البكتيريا positive أو negative للجيلاتيناز، يبقى
+
+386
+00:29:18,670 --> 00:29:24,030
+ممنوع أضع أجار، لأن لو وضعت أجار الـ media ستظل صلبة
+
+387
+00:29:24,030 --> 00:29:26,230
+سواء كانت البكتيريا positive أو negative
+
+388
+00:29:26,230 --> 00:29:33,710
+للجيلاتيناز، أضيف 12% جيلاتين، يبقى الـ 12% الجيلاتين
+
+389
+00:29:33,710 --> 00:29:38,910
+سيكون كـ substrate، كيف أُحضّر هذه الـ media؟
+
+390
+00:29:38,910 --> 00:29:44,250
+أقول لك، تأخذين الـ nutrient broth، تمام، تقرئين أيّة
+
+391
+00:29:44,250 --> 00:29:48,270
+التعليمات الموجودة على العبوة، مكتوب لي أن كل
+
+392
+00:29:48,270 --> 00:29:52,190
+1000 مل أضيف إليها 13 جرام من الـ nutrient broth، طيب
+
+393
+00:29:52,190 --> 00:29:56,860
+أنا أريد أن أُحضّر 500 مل، أفعل ضرب تبادلي، يظهر أنني
+
+394
+00:29:56,860 --> 00:30:00,860
+أحتاج 6.5 جرام من الـ nutrient broth، طيب نسبة
+
+395
+00:30:00,860 --> 00:30:06,400
+الجيلاتين، أقول لك المستخدمة في هذا الفحص 12%، 12%
+
+396
+00:30:06,400 --> 00:30:11,900
+يعني 12 لكل 100 مل distilled water، طيب أنا أضفت 500 مل،
+
+397
+00:30:11,900 --> 00:30:16,220
+أفعل ضرب تبادلي، يبقى أحتاج 60 جرام من الجيلاتين، يبقى
+
+398
+00:30:16,220 --> 00:30:20,540
+أضيف الـ 60 جرام من الجيلاتين على
+
+445
+00:34:08,910 --> 00:34:14,050
+قليلة فبالتالي عشان تقدر تكسر الجيلاتين بياخد وقت
+
+446
+00:34:14,050 --> 00:34:18,610
+فأنا عشان أضمن أن هذه البكتيريا لو كان في عندي
+
+447
+00:34:18,610 --> 00:34:21,750
+هذه البكتيريا بتفرز الإنزيم بكميات قليلة عشان أضمن
+
+448
+00:34:21,750 --> 00:34:27,510
+هذا الشيء بخليها incubation لمدة 30 يومًا بعد ما يخلص
+
+449
+00:34:27,510 --> 00:34:32,570
+الـ 30 يوم، بحطها في التلاجة لمدة 30 minute لنفس
+
+450
+00:34:32,570 --> 00:34:36,490
+السبب الذي ذكرناه قبل شوية، لأنه سواء كانت positive
+
+451
+00:34:36,490 --> 00:34:39,870
+أو negative، هتكون liquid لأن درجة حرارة الـ
+
+452
+00:34:39,870 --> 00:34:45,310
+incubator 37°C. لو كانت negative فبالتالي الجيلاتين
+
+453
+00:34:45,310 --> 00:34:48,550
+فيه، لما تكون درجة الحرارة أعلى من 26°C، هيكون في
+
+454
+00:34:48,550 --> 00:34:52,610
+الحالة في الـ liquid، في الحالة السائلة. لو كان عندها
+
+455
+00:34:52,610 --> 00:34:57,850
+الـ enzyme أكيد هيتكسر. فأنا مش عارفة هل عندها الإنزيم
+
+456
+00:34:57,850 --> 00:35:04,810
+ولا لأ، فأحطها في التلاجة لمدة 30 دقيقة. إذا دلت
+
+457
+00:35:04,810 --> 00:35:08,930
+liquid يبقى هي positive لكن لو اتصلبت يبقى هي
+
+458
+00:35:08,930 --> 00:35:13,010
+negative. نسجل النتيجة negative. هذا بالنسبة
+
+459
+00:35:13,010 --> 00:35:17,110
+للجيلاتين step method. الفكرة، الـ principle زي ما
+
+460
+00:35:17,110 --> 00:35:22,950
+قلنا، أنه الوسط بتتحول لـ liquid. هذا الـ principle
+
+461
+00:35:23,500 --> 00:35:28,860
+وبالنسبة للـ Result إما Liquid أو Solid. الـ Result
+
+462
+00:35:28,860 --> 00:35:33,720
+في الجيلاتين stab method، لو كانت نتيجة Positive
+
+463
+00:35:33,720 --> 00:35:38,020
+بيكون Liquid. بتنتج وينتهي عن أنزيم الجيلاتيناز ممكن
+
+464
+00:35:38,020 --> 00:35:41,480
+يكون Strong Positive وممكن تكون Weak Positive.
+
+465
+00:35:41,480 --> 00:35:46,040
+Strong Positive: إنها بتتحول لـ Liquid خلال تلت
+
+466
+00:35:46,040 --> 00:35:50,840
+أيام. لكن لو كانت weak positive خلال من تلت لتلتين
+
+467
+00:35:50,840 --> 00:35:54,420
+يوم، بيصير عندي اللي هو بتحول الوسط لـ liquid زي
+
+468
+00:35:54,420 --> 00:35:59,380
+البروتياز. قلنا أنه بيكون عندها الإنزيم، الإنزيم
+
+469
+00:35:59,380 --> 00:36:03,220
+بكميات قليلة. الـ negative: أن الوسط بيصير له
+
+470
+00:36:03,220 --> 00:36:07,460
+solidify، بتصلب نتيجة أن الجيلاتين ما تكسرش، دلو
+
+471
+00:36:07,460 --> 00:36:12,640
+الجيلاتين مع إنزيم الـ Gelatinase حتى بعد 30 يومًا
+
+472
+00:36:12,640 --> 00:36:17,600
+بتظل الـ media still solid. هذا بالنسبة لنتائج
+
+473
+00:36:17,600 --> 00:36:24,080
+طريقة الـ Gelatin Stab Method. نمر على الـ slides في
+
+474
+00:36:24,080 --> 00:36:27,660
+الجيلاتين step method. بقول لي: أنكولات نيوتريان
+
+475
+00:36:27,660 --> 00:36:31,900
+جيلاتين، تيوب بتحتوي على 12% جيلاتين. بعملها
+
+476
+00:36:31,900 --> 00:36:36,740
+incubation لمدة أسبوع. بحدد إذا صار في عندي
+
+477
+00:36:36,740 --> 00:36:40,880
+liquification، أنه اتحول الـ media لـ liquid ولا لأ.
+
+478
+00:36:40,880 --> 00:36:47,700
+أو بحطها في التلاجة لمدة 30 دقيقة. بعدها بشوف، بعد ما
+
+479
+00:36:47,700 --> 00:36:51,020
+أتخلص من الثلاثين دقيقة، بتأكد أنه صار في عندي
+
+480
+00:36:51,020 --> 00:36:54,600
+liquefaction ولا لأ. بقول: لو negative، بتكملي
+
+481
+00:36:54,600 --> 00:36:59,140
+incubation لثلاثين يومًا. if the gelatin is still
+
+482
+00:36:59,140 --> 00:37:02,640
+intact، لو الجيلاتين ظل صلب، يبقى البكتيريا
+
+483
+00:37:02,640 --> 00:37:06,540
+didn't produce gelatinase، البكتيريا ما عندها إنزيم
+
+484
+00:37:06,540 --> 00:37:11,030
+الجيلاتيناز. الـ media will solidify، الـ media بتتصلب
+
+485
+00:37:11,030 --> 00:37:14,250
+في التلاجة، والـ negative result أنا بسجلها
+
+486
+00:37:14,250 --> 00:37:18,330
+negative result. إنه لو أنا حاطيتها في التلاجة تمام،
+
+487
+00:37:18,330 --> 00:37:24,350
+الـ media هتتصلب لأنه درجة الحرارة أقل من 25°C. قلنا
+
+488
+00:37:24,350 --> 00:37:28,310
+أنه الجيلاتين بيتغير حسب درجة الحرارة. if the
+
+489
+00:37:28,310 --> 00:37:32,410
+organism has produced sufficient gelatinase، لو كان
+
+490
+00:37:32,410 --> 00:37:36,970
+عند الـ organism إنزيم الجيلاتيناز، التيوب سيظل
+
+491
+00:37:36,970 --> 00:37:40,790
+liquid حتى
+
+492
+00:37:40,790 --> 00:37:42,630
+لو أنا حطيته في التلاجة.
+
+493
+00:37:49,500 --> 00:37:53,920
+إذا بنلاحظ أنه أنا ميّلت، فبت... ميّلت ال... التيوب
+
+494
+00:37:53,920 --> 00:37:58,960
+فالتيوب ما اتحرك، يبقى ظل الوسط solid، يبقى هي
+
+495
+00:37:58,960 --> 00:38:03,260
+negative gelatinase. لكنها ميّلت التيوب، يبقى اتحرك
+
+496
+00:38:03,260 --> 00:38:08,920
+يبقى الوسط سائل، يبقى هي positive gelatinase. some
+
+497
+00:38:08,920 --> 00:38:13,150
+organism may produce gelatinase in rather small
+
+498
+00:38:13,150 --> 00:38:18,030
+quantities. قلنا في بعض أنواع البكتيريا تفرز إنزيم
+
+499
+00:38:18,030 --> 00:38:22,210
+الجيلاتيناز بكميات قليلة. عشان هيك الـ negative
+
+500
+00:38:22,210 --> 00:38:26,150
+gelatinase لازم إني أنا أعملها re-incubated for 30
+
+501
+00:38:26,150 --> 00:38:30,850
+days، لمدة 30 يومًا لازم أعمل incubation عشان أتأكد
+
+502
+00:38:30,850 --> 00:38:38,310
+أنه هي negative result بالنسبة
+
+503
+00:38:38,310 --> 00:38:42,640
+لهذا الـ slide. قلنا السيراشيا عبارة عن positive
+
+504
+00:38:42,640 --> 00:38:46,680
+للجيلاتين. بقدر أفرق بينها وبين باقي الـ
+
+505
+00:38:46,680 --> 00:38:50,400
+Enterobacteriaceae. السلمونيلا هي عبارة عن من عائلة الـ
+
+506
+00:38:50,400 --> 00:38:52,720
+Enterobacteriaceae. قلنا إحنا فقط من عائلة الـ
+
+507
+00:38:52,720 --> 00:38:56,100
+Enterobacteriaceae الجيلاتين positive. السيراشيا والـ
+
+508
+00:38:56,100 --> 00:38:59,320
+بروتيوس يبقى السلمونيلا هي بتكون negative
+
+509
+00:38:59,320 --> 00:39:04,040
+الميديا اتصلبت. لكن هنا في السيراشيا هي positive
+
+510
+00:39:04,040 --> 00:39:08,520
+للجيلاتين. بقول لنا أن الميديا صارت liquid، سائلة. إذا
+
+511
+00:39:08,520 --> 00:39:13,600
+ملاحظتها اليوم، في عندي سائل اتحرك التيوب لكنها صلبة
+
+512
+00:39:13,600 --> 00:39:20,480
+الميديا يبقى هي بتكون negative للجيلاتيناز. النتائج
+
+513
+00:39:20,480 --> 00:39:23,660
+قلنا ممكن أنها تكون positive strong أو weak. الـ
+
+514
+00:39:23,660 --> 00:39:27,120
+strong خلال ثلاث أيام. الـ weak من ثلاثة لثلاثين يومًا.
+
+515
+00:39:27,120 --> 00:39:31,320
+الـ negative حتى بعد ثلاثين يومًا ما بيصير في عندي
+
+516
+00:39:31,320 --> 00:39:34,360
+liquefaction، بتظل الـ media solid.
+
+517
+00:39:38,650 --> 00:39:42,750
+هذه الطريقة الأولى شرحناها. نجي للطريقة الثانية
+
+518
+00:39:42,750 --> 00:39:45,910
+اللي هي الـ Gelatin strip method. الـ Gelatin strip
+
+519
+00:39:45,910 --> 00:39:49,190
+method بقول لنا بنجيب تيوب بنحط فيها bacterial
+
+520
+00:39:49,190 --> 00:39:51,670
+suspension. إيش يعني bacterial suspension؟ يعني
+
+521
+00:39:51,670 --> 00:39:54,690
+normal saline مع بكتيريا أو nutrient broth مع
+
+522
+00:39:54,690 --> 00:39:57,790
+بكتيريا. طبعًا البكتيريا اللي بدي أُكشف عندها الإنزيم
+
+523
+00:39:57,790 --> 00:40:02,450
+ولا لأ. بقول لنا بنضيف X-ray film. الـ X-ray film
+
+524
+00:40:02,450 --> 00:40:06,210
+بقول لنا هي coated with green gelatin emulsion. يبقى
+
+525
+00:40:06,210 --> 00:40:10,490
+هتكون هي الـ substrate. الـ X-ray film بتحتوي على
+
+526
+00:40:10,490 --> 00:40:13,370
+الجيلاتين، يبقى هي الـ substrate اللي هيشتغل عليها
+
+527
+00:40:13,370 --> 00:40:16,210
+الـ إنزيم الموجود في البكتيريا أو مش موجود، أنا بدي
+
+528
+00:40:16,210 --> 00:40:22,870
+أكشف عنه. بعدها بعمل incubation على درجة 37°C لمدة 4
+
+529
+00:40:22,870 --> 00:40:27,590
+ساعات. لو البكتيريا عندها إنزيم الجيلاتيناز، هتشتغل
+
+530
+00:40:27,590 --> 00:40:32,370
+على الجيلاتين الموجود بالـ X-ray، وهتحاول إنها إيش
+
+531
+00:40:32,370 --> 00:40:36,470
+تكسر هذا الجيلاتين. فبالتالي اللون الأسود اللي
+
+532
+00:40:36,470 --> 00:40:42,250
+موجود على فيلم الـ X-ray هيختفي، وهيصير colorless.
+
+533
+00:40:42,250 --> 00:40:46,010
+يبقى النتيجة الـ positive: أن الـ X-ray film هيسود
+
+534
+00:40:46,010 --> 00:40:49,490
+دي سبير، هيصير colorless. لكن لو ظل لونه أسود
+
+535
+00:40:49,490 --> 00:40:53,740
+بقول: ما تسجل النتيجة negative. بتعمل Re-incubation
+
+536
+00:40:53,740 --> 00:40:58,660
+for a total of 24 hours. بتكمل الـ Incubation لـ 24
+
+537
+00:40:58,660 --> 00:41:02,420
+ساعة. يبقى لما إحنا حطينا بالأول 4 ساعات، بنعمل
+
+538
+00:41:02,420 --> 00:41:06,300
+الـ Incubation لمدة 20 ساعة. لو ظل لونه أسود يبقى
+
+539
+00:41:06,300 --> 00:41:12,020
+خلاص بسجل النتيجة negative. لكن لو اتغير للـ، للـ
+
+540
+00:41:12,020 --> 00:41:15,540
+colorless، اختفى اللون الأسود وصار colorless يبقى هي
+
+541
+00:41:15,540 --> 00:41:20,070
+positive للجيلاتيناز. لكن أخذ وقت لأن اللي قلنا في
+
+542
+00:41:20,070 --> 00:41:24,850
+بعض أنواع البكتيريا بتفرز الإنزيم بكميات قليلة. هذا
+
+543
+00:41:24,850 --> 00:41:28,630
+بالنسبة لطريقة الـ Gelatin strip method، هي
+
+544
+00:41:28,630 --> 00:41:31,710
+alternative method for detecting gelatinase
+
+545
+00:41:31,710 --> 00:41:35,190
+production. طريقة ثانية عشان أفحص عن الجيلاتيناز
+
+546
+00:41:35,190 --> 00:41:40,080
+باستخدام الـ X-ray film. الـ Gelatin strip بقول لنا
+
+547
+00:41:40,080 --> 00:41:44,600
+الجيلاتين الـ X-ray film that is coated with a
+
+548
+00:41:44,600 --> 00:41:48,140
+green gelatin emulsion. هي بتكون فيها اللي هو
+
+549
+00:41:48,140 --> 00:41:52,480
+الجيلاتين. organism that produce gelatinase remove
+
+550
+00:41:52,480 --> 00:41:56,080
+بقول لي: لو البكتيريا عندها إنزيم الجيلاتيناز، هتزيل
+
+551
+00:41:56,080 --> 00:41:59,780
+الـ emulsion from the strip، هتزيل اللون الأسود من
+
+552
+00:41:59,780 --> 00:42:03,000
+الـ strip. كباقي الطريقة زي ما قلنا بعمل أنكولات
+
+553
+00:42:03,000 --> 00:42:08,790
+للبكتيريا في نصف مل من الـ normal saline. لازم يكون الـ
+
+554
+00:42:08,790 --> 00:42:11,770
+suspension مترابطة. بعدين بحط الـ X-ray film أو
+
+555
+00:42:11,770 --> 00:42:16,330
+الجيلاتين في الـ bacterial suspension أو الـ saline
+
+556
+00:42:16,330 --> 00:42:20,610
+suspension. بعمل incubation لمدة أربع ساعات لأربع
+
+557
+00:42:20,610 --> 00:42:25,690
+عشرون ساعة عشان ألاحظ إزالة اللي هو الجيلاتين
+
+558
+00:42:25,690 --> 00:42:34,070
+emulsion من الـ strip. من اللاحظ في هذه الصورة، ما
+
+559
+00:42:34,070 --> 00:42:38,650
+اختفى اللون الأسود من الـ Strip، يبقى هذه النتيجة
+
+560
+00:42:38,650 --> 00:42:43,370
+Negative للجيلاتيناز. لكن هنا نلاحظ أن الـ Strip
+
+561
+00:42:43,370 --> 00:42:47,370
+راح اللون الأسود منه، يبقى هذه Gelatinase Positive.
+
+562
+00:42:48,140 --> 00:42:52,180
+الجزء اللي فوق طبعًا أكيد ما راح اللون لأنه ما صار
+
+563
+00:42:52,180 --> 00:42:55,980
+فيه touch بينه وبين البكتيريا. البكتيريا موجودة في
+
+564
+00:42:55,980 --> 00:42:59,960
+الـ suspension في الجزء اللي تحت. فأكيد اللي صار فيه
+
+565
+00:42:59,960 --> 00:43:03,400
+touch هو اللي هيروح، هيصير التفاعل مع الـ substrate
+
+566
+00:43:03,400 --> 00:43:06,540
+وهيروح اللون من الـ strip، يبقى high gelatinase
+
+567
+00:43:06,540 --> 00:43:11,710
+positive، و high gelatinase positive. خلّصنا طريقة اللي
+
+568
+00:43:11,710 --> 00:43:14,990
+هي الـ Gelatin Strip. نجي لآخر طريقة اللي هي الـ
+
+569
+00:43:14,990 --> 00:43:19,470
+charcoal method. الـ charcoal هو الفحم. الفكرة أن
+
+570
+00:43:19,470 --> 00:43:25,070
+الفحم جزيئات متماسكة، وسبب وجود الجيلاتين فيه. يبقى
+
+571
+00:43:25,070 --> 00:43:27,890
+بجيب أنا bacterial suspension، normal saline مع
+
+572
+00:43:27,890 --> 00:43:31,270
+بكتيريا أو nutrient broth مع بكتيريا. البكتيريا بدي
+
+573
+00:43:31,270 --> 00:43:35,330
+أكشف موجود فيها الإنزيم ولا لأ. فبجيب charcoal، فحم، و
+
+574
+00:43:35,330 --> 00:43:39,480
+أحطه فيه. high lithium بدل الـ X-ray film. القطعة
+
+575
+00:43:39,480 --> 00:43:43,900
+charcoal هيكون فيه الـ substrate، تمام. هعمل incubation
+
+576
+00:43:43,900 --> 00:43:49,100
+لمدة 24 ساعة على 37°C. إذا لاحظت أنه
+
+577
+00:43:49,100 --> 00:43:52,480
+الفحم أترسب في قاع التيوب، يبقى النتيجة negative.
+
+578
+00:43:52,480 --> 00:43:57,020
+لكن إذا لاحظت أنه الفحم اتوزع في كل التيوب، اتفكك
+
+579
+00:43:57,020 --> 00:44:01,730
+الفحم، هيصير انتشار في كل تيوب، هيتغير لون التيوب لـ
+
+580
+00:44:01,730 --> 00:44:06,350
+اللون الأسود، يبقى نتيجة positive لأن البكتيريا
+
+581
+00:44:06,350 --> 00:44:10,370
+عندها إنزيم الجيلاتيناز كسر الجيلاتين. الجيلاتين هو
+
+582
+00:44:10,370 --> 00:44:14,130
+اللي بيخلي الفحم متماسك. طيب الجيلاتين اتكسر، راح
+
+583
+00:44:14,130 --> 00:44:17,910
+يبقى الفحم هينتشر في كل تيوب، وهيصير لونه أسود، يبقى
+
+584
+00:44:17,910 --> 00:44:21,710
+النتيجة الـ positive. distribution هيصير في عندي لون
+
+585
+00:44:21,710 --> 00:44:25,810
+أسود في كل تيوب. لكن نتيجة الـ negative، مش هتفرز
+
+586
+00:44:25,810 --> 00:44:30,250
+إنزيم الجيلاتيناز، يبقى الجيلاتين هيضل بين جزيئات
+
+587
+00:44:30,250 --> 00:44:34,790
+الفحم، فبالتالي هيرسب في القاع. هذه فكرة. let's
+
+588
+00:44:34,790 --> 00:44:41,410
+share cool. هكذا خلصنا طُرُق الجيلاتيناز، وخلصنا الـ
+
+589
+00:44:41,410 --> 00:44:44,770
+chapter اللي كان بيتكلم عن الأميليز و الجيلاتيناز
+
+590
+00:44:44,770 --> 00:44:50,270
+وقلنا إثنين exoenzyme. بتمنى تكون الأمور واضحة.
+
+591
+00:44:50,270 --> 00:44:53,070
+والسلام عليكم ورحمة الله وبركاته.
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/YWBUtjE9yHc_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/YWBUtjE9yHc_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..96915543ac506cb19942c7293ecb001c9f82ea5f
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/YWBUtjE9yHc_postprocess.srt
@@ -0,0 +1,2364 @@
+1
+00:00:02,090 --> 00:00:05,750
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:05,750 --> 00:00:09,190
+جميعا في هذا اللقاء نستكمل سلسلة ال biochemical
+
+3
+00:00:09,190 --> 00:00:12,910
+test في المحاضرة السابقة اتكلمنا على الانزيمات
+
+4
+00:00:12,910 --> 00:00:16,090
+وقسمنا الانزيم حسب ال side of production ل
+
+5
+00:00:16,090 --> 00:00:19,930
+endoenzyme و exoenzyme ال endoenzyme قلنا انه
+
+6
+00:00:19,930 --> 00:00:24,670
+التفاعل بيصير intracellular داخل الخليةلكن الـ
+
+7
+00:00:24,670 --> 00:00:28,590
+Exoenzyme التفاعل بيصير Extracellular خارج الخلية
+
+8
+00:00:28,590 --> 00:00:33,770
+في ال Exoenzyme الإنزيم يفرز داخل الخلية لكن بيطلع
+
+9
+00:00:33,770 --> 00:00:37,150
+بر الخلية لأن ال substrate اللي بيشتغل عليها ال
+
+10
+00:00:37,150 --> 00:00:42,670
+Exoenzyme حجمها كبير high molecular weight يبقى ال
+
+11
+00:00:42,670 --> 00:00:45,490
+substrate اللي هيشتغل عليها ال Exoenzyme high
+
+12
+00:00:45,490 --> 00:00:49,430
+molecular weight such as polysaccharide lipid and
+
+13
+00:00:49,430 --> 00:00:53,850
+proteinهدول يعتبروا macromolecules ما بيقدروا
+
+14
+00:00:53,850 --> 00:00:57,970
+يدخلوا جوا الخلية عشان هيك لازم يطلع الانزيم لبرا
+
+15
+00:00:57,970 --> 00:01:01,870
+ويصير التفاعل extracellular من الأمثل على
+
+16
+00:01:01,870 --> 00:01:04,970
+البوليساكرايد ال starch من الأمثل على الليبد ال
+
+17
+00:01:04,970 --> 00:01:09,550
+triglyceride من الأمثل على ال protein fibrinogen
+
+18
+00:01:09,550 --> 00:01:14,830
+casein gelatin الamylase انزيم بيشتغل على ال
+
+19
+00:01:14,830 --> 00:01:19,070
+starchالـ lipase بيشتغل على الـ triglyceride الـ
+
+20
+00:01:19,070 --> 00:01:22,170
+coagulase أخدناها في المحاضرة السابقة قولنا هو
+
+21
+00:01:22,170 --> 00:01:28,670
+إيجزو أنزايم بيشتغل على الفيبرنوجين الكازيناز
+
+22
+00:01:28,670 --> 00:01:32,310
+قولنا بيشتغل على الكازين الجيلاتيناز بيشتغل على
+
+23
+00:01:32,310 --> 00:01:37,270
+الجيلاتينهدول كلهم يعتبروا exo-enzyme لأن ال
+
+24
+00:01:37,270 --> 00:01:42,610
+substrate اللي بيشتغله عليها تعتبر high molecular
+
+25
+00:01:42,610 --> 00:01:46,670
+weight حجمها كبير ما بتقدر تدخل لداخل الخلية عشان
+
+26
+00:01:46,670 --> 00:01:51,990
+هي ال enzymات بتفرس داخل الخلية و تطلع لبرا عشان
+
+27
+00:01:51,990 --> 00:01:57,470
+يصير التفاعل extra cellular لو رجعنا لسلايدة type
+
+28
+00:01:57,470 --> 00:02:01,790
+of enzyme حسب ال site of productionفي الـ
+
+29
+00:02:01,790 --> 00:02:07,130
+Exoenzyme كان ذاكر أمثلة هنلاقي هذه الانزيمات هي
+
+30
+00:02:07,130 --> 00:02:13,350
+مذكورة في الجملة يبقى عشان نحفظ الـ Exoenzyme بشوف
+
+31
+00:02:13,350 --> 00:02:17,590
+شو ال substrate اللي بيشتغل عليها ال enzyme إذا ال
+
+32
+00:02:17,590 --> 00:02:22,510
+substrate polysaccharide or lipid or protein يبقى
+
+33
+00:02:22,510 --> 00:02:25,750
+هاي تعتبر high molecular weight يبقى ال enzyme
+
+34
+00:02:25,750 --> 00:02:30,160
+يعتبر Exoenzymeاليوم ان شاء الله في هذه المحاضرة
+
+35
+00:02:30,160 --> 00:02:35,580
+هنتكلم عن الاميلاز and جيلاتيناز هنبدأ بالاميلاز
+
+36
+00:02:35,580 --> 00:02:40,340
+production or starch hydrolysis test أول معلومة
+
+37
+00:02:40,340 --> 00:02:45,600
+عرفناها عن الاميلازإنه Exoenzyme السبسترات اللي
+
+38
+00:02:45,600 --> 00:02:49,680
+بيشتغل عليها ال amylase هي ال starch ال starch
+
+39
+00:02:49,680 --> 00:02:54,940
+يعتبر polysaccharide ال amylase بيعمل hydrolysis ل
+
+40
+00:02:54,940 --> 00:02:59,560
+ال starch عشان هي اسم الفحص starch hydrolysis test
+
+41
+00:02:59,560 --> 00:03:03,540
+or amylase production لأنه بيعمل hydrolysis لل
+
+42
+00:03:03,540 --> 00:03:07,600
+starch ال starch عبارة عن its chain of
+
+43
+00:03:07,600 --> 00:03:12,150
+monosaccharideمن الـ monosaccharide الجليكوز لو
+
+44
+00:03:12,150 --> 00:03:16,730
+بدنا نتعرف على الـ type of sugar الـ types of
+
+45
+00:03:16,730 --> 00:03:20,410
+sugar فيه عندى monosaccharide, disaccharide و
+
+46
+00:03:20,410 --> 00:03:25,370
+polysaccharide الـ monosaccharide جليكوز galactose
+
+47
+00:03:25,370 --> 00:03:29,050
+افراكتوز هي أمثلة على monosaccharide الـ
+
+48
+00:03:29,050 --> 00:03:33,150
+disaccharide بتتكون من two monosaccharide مرتبطين
+
+49
+00:03:33,150 --> 00:03:37,900
+من الأمثلة على الـ disaccharideSaccharose,
+
+50
+00:03:38,020 --> 00:03:41,680
+Maltose, Lactose الـ Saccharose عبارة عن جليكوز
+
+51
+00:03:41,680 --> 00:03:46,320
+and فركتوز المالتوز عبارة عن جليكوز زائد جليكوز
+
+52
+00:03:46,320 --> 00:03:51,200
+اللاكتوز جليكوز زائد جلاكتوز تالت نوع الـ
+
+53
+00:03:51,200 --> 00:03:54,720
+Polysaccharide مثال عليها الـ starch قولنا أن الـ
+
+54
+00:03:54,720 --> 00:03:59,560
+starch عبارة عن polymer of مونو ساكرايد من المونو
+
+55
+00:03:59,560 --> 00:04:03,080
+ساكرايد الجليكوز من الأمثلة الأخرى على ال
+
+56
+00:04:03,080 --> 00:04:06,620
+polysaccharide في عندي الجليكوجين وعندي السليلوز
+
+57
+00:04:07,200 --> 00:04:11,360
+لكن اليوم ان شاء الله هنتكلم احنا عن ال amylase
+
+58
+00:04:11,360 --> 00:04:15,420
+اللى بيشتغل على ال starch ال starch يعتبر
+
+59
+00:04:15,420 --> 00:04:20,140
+polysaccharide بتكون من polymer of glycose ال
+
+60
+00:04:20,140 --> 00:04:24,380
+starch هيدروليزز تكسير ال starch بيصير بواسط ال
+
+61
+00:04:24,380 --> 00:04:29,400
+two type of enzyme بواسط اتنوعين من الانزيمات اول
+
+62
+00:04:29,400 --> 00:04:35,060
+انزيم Alpha amylase Alpha وليس beta لأن ال betaيله
+
+63
+00:04:35,060 --> 00:04:38,420
+علاقة بالـ cellulose الـ cellulose الموجود في
+
+64
+00:04:38,420 --> 00:04:44,980
+البلاند الـ alpha amylase بيعمل hydrolysis of α1,4
+
+65
+00:04:44,980 --> 00:04:49,880
+-glycosidic bond بيكسر الرابطة اللي اسمها α1,4
+
+66
+00:04:49,880 --> 00:04:54,520
+-glycosidic bond الانزيم التاني المسؤول عن تكسير
+
+67
+00:04:54,520 --> 00:04:59,880
+ال starch Oligo-1,6-glycosidase مسؤول عن تكسير
+
+68
+00:04:59,880 --> 00:05:05,920
+الرابطة α1,6-glycosidic bondعشان نوضح الصورة اكتر
+
+69
+00:05:05,920 --> 00:05:09,000
+هذه
+
+70
+00:05:09,000 --> 00:05:14,820
+الصورة بتوضح الـ starch structure الـ starch قولنا
+
+71
+00:05:14,820 --> 00:05:18,240
+عبارة عن its chain of monosaccharide الـ
+
+72
+00:05:18,240 --> 00:05:21,360
+monosaccharide اللي هو الجليكوز يبقى هو عبارة عن
+
+73
+00:05:21,360 --> 00:05:25,760
+سلسلة من الجليكوز هاي الوحدات الخضراء عبارة عن
+
+74
+00:05:25,760 --> 00:05:32,640
+الجليكوزإذا كان ال ال structure chain الرابط ما
+
+75
+00:05:32,640 --> 00:05:36,900
+بينهم بتكون alpha one four linkage لو كان في عندي
+
+76
+00:05:36,900 --> 00:05:41,620
+branch بتكون الرابط alpha one six linkage احنا
+
+77
+00:05:41,620 --> 00:05:46,900
+قلنا انه في عندي two type two enzyme مسئولين عن ال
+
+78
+00:05:46,900 --> 00:05:51,020
+starch hydrolysis الانزيم الأول اللي هو ال alpha
+
+79
+00:05:51,020 --> 00:05:54,720
+amylase ال alpha amylase قلنا مسئول عن تكسير ال
+
+80
+00:05:55,030 --> 00:05:59,410
+الرابطة الهيدروليسيز للرابط ألفة وان فار في
+
+81
+00:05:59,410 --> 00:06:03,550
+جلايكوسيدكبون يبقى ال alpha amylase هيجي هنا في
+
+82
+00:06:03,550 --> 00:06:08,030
+حال كان في عندي chain في عندي سلسلة هيجي يكسر هاي
+
+83
+00:06:08,030 --> 00:06:12,950
+الروابط ويعطيني اما لو كان اعطا لو ايجي هنا من هاي
+
+84
+00:06:12,950 --> 00:06:17,530
+الرابطة لو
+
+85
+00:06:17,530 --> 00:06:23,430
+كسر هنا اتنين جليكوز أداني مالتوز لو كسر هناهيديني
+
+86
+00:06:23,430 --> 00:06:29,950
+تلاتة جيليكوز بسميهم malto triose تمام لو اتكسرت
+
+87
+00:06:29,950 --> 00:06:33,630
+هان الرابط هذا المركب اذا ضال ال branch بسميه
+
+88
+00:06:33,630 --> 00:06:39,030
+dextrine هيجي ال alpha او oligo alpha 16
+
+89
+00:06:39,030 --> 00:06:43,670
+glycosidase هيكسر الرابط اللي هان اللي هي alpha 16
+
+90
+00:06:43,670 --> 00:06:49,170
+linkage ويديني maltose and maltotriose هذه كلها
+
+91
+00:06:49,170 --> 00:06:55,120
+المركباتعبارة عن law molecular weight soluble in
+
+92
+00:06:55,120 --> 00:06:58,980
+water بتقدر تدخل للخلية عبر ال cell membrane
+
+93
+00:06:58,980 --> 00:07:03,600
+تستخدم في ال energy production خلال عملية ال
+
+94
+00:07:03,600 --> 00:07:08,140
+glycolysis كمان تستخدم ك carbon source ال carbon
+
+95
+00:07:08,140 --> 00:07:11,580
+source طبعا عندي أنا ال carbohydrate هي من
+
+96
+00:07:11,580 --> 00:07:18,060
+الفائدتها اللي هي تعتبر كمصدر للكربون اللازم لنمو
+
+97
+00:07:18,060 --> 00:07:21,530
+البكتيريايبقى هاي كلها تعتبر اللي هو molecular
+
+98
+00:07:21,530 --> 00:07:26,450
+weight بتقدر تدخل للخلية الخلية بتقدر تستفيد منها
+
+99
+00:07:26,450 --> 00:07:31,470
+ممكن كمصدر للطاقة أو ممكن كمصدر للكربون اللازم
+
+100
+00:07:31,470 --> 00:07:39,070
+لنمو البكتيري نرجع
+
+101
+00:07:39,070 --> 00:07:45,020
+لأول slideStarch agar is a differential medium لما
+
+102
+00:07:45,020 --> 00:07:49,980
+قسمنا ال media حسب ال function قلنا انه في عندي
+
+103
+00:07:49,980 --> 00:07:53,460
+differential media ال differential media بتعطي كل
+
+104
+00:07:53,460 --> 00:07:58,180
+بكتيريا لون بالتالي بتميز بكتيريا عن بكتيريا وهذا
+
+105
+00:07:58,180 --> 00:08:01,940
+ان شاء الله هنشوفه في ال result that test the
+
+106
+00:08:01,940 --> 00:08:05,140
+ability of an organism to produce certain
+
+107
+00:08:05,140 --> 00:08:09,640
+exoenzyme ولي هذا الفحص بيكشف عن قدرة ال organism
+
+108
+00:08:10,200 --> 00:08:14,500
+إنه ينتج Certain Exoenzyme قولنا إن الـ Amylase
+
+109
+00:08:14,500 --> 00:08:19,220
+يعتبر Exoenzyme الإنزيمات المسؤولة عن الـ starch
+
+110
+00:08:19,220 --> 00:08:23,020
+Hydrolysis أول إنزيم اللي هو الـ Alpha Amylase
+
+111
+00:08:23,020 --> 00:08:27,120
+المسؤول عن تكسير الرابط Alpha-1-Pharyglycosidic
+
+112
+00:08:27,120 --> 00:08:30,840
+bond الإنزيم التاني يبقى هذا أول إنزيم اللي هو الـ
+
+113
+00:08:30,840 --> 00:08:31,840
+Alpha Amylase
+
+114
+00:08:35,090 --> 00:08:39,810
+الانزيم التاني اللي هو Oligo-1,6-Glycosidase
+
+115
+00:08:39,810 --> 00:08:45,570
+المسؤول عن تكسير الرابطة Alpha-1,6-Glycosidic bond
+
+116
+00:08:45,570 --> 00:08:50,790
+that hydrolyze starch بيكسروا ال starch لمركبات
+
+117
+00:08:50,790 --> 00:08:56,130
+صغيرة الحجم بتقدر تدخل لخلية starch molecules are
+
+118
+00:08:56,130 --> 00:08:59,490
+too large to enter the bacterial cell قولنا إن ال
+
+119
+00:08:59,490 --> 00:09:03,670
+starch يعتبر polysaccharide حجمه كبير ما بيقدر
+
+120
+00:09:03,670 --> 00:09:08,710
+يدخل الخليةSo some bacteria some مش كل البكتيريا
+
+121
+00:09:08,710 --> 00:09:13,410
+بتفرز الانزيمات المسؤولة عن تكسير ال starch some
+
+122
+00:09:13,410 --> 00:09:18,110
+bacteria secrete exoenzyme اتفقنا انه عبارة عن
+
+123
+00:09:18,110 --> 00:09:22,550
+exoenzyme to degrade starch into subunit بيكسل ال
+
+124
+00:09:22,550 --> 00:09:25,870
+starch ل subunit زي ما اتعرفنا اما مالتوز مالتو
+
+125
+00:09:25,870 --> 00:09:30,550
+تريوز او ديسترين هاي ال subunitthat can then be
+
+126
+00:09:30,550 --> 00:09:33,810
+utilized by the organism بتقدر تدخل للخلية
+
+127
+00:09:33,810 --> 00:09:43,090
+والorganism يقدر انه يستهلكها ويستفيد منها هيك
+
+128
+00:09:43,090 --> 00:09:48,930
+احنا عرفنا كيف الانزيم بيكسر ال starch كيف شغل
+
+129
+00:09:48,930 --> 00:09:52,930
+الانزيم طب كيف بدنا نكشف عن هذا الانزيم وهل
+
+130
+00:09:52,930 --> 00:09:59,270
+البكتيريا قادرة انها تكسر ال starch او لأأحنا قلنا
+
+131
+00:09:59,270 --> 00:10:04,190
+إن أي أنزيم بيشتغل على substrates الأميلاز بيشتغل
+
+132
+00:10:04,190 --> 00:10:09,170
+على ال starch يبقى عشان أكشف على الأميلاز لازم
+
+133
+00:10:09,170 --> 00:10:14,670
+أزرع البكتيريا على ميديا فيها starch starch agar
+
+134
+00:10:14,670 --> 00:10:20,350
+is a simple nutrative media بقول هي عبارة عن media
+
+135
+00:10:20,350 --> 00:10:24,830
+simple with starch added يبقى هي عبارة عن nutrient
+
+136
+00:10:24,830 --> 00:10:29,730
+agar مضاف إليها ال starch as supplementالسترش هو
+
+137
+00:10:29,730 --> 00:10:32,890
+عبارة عن ال substrate اللي هيشتغل عليه الانزين
+
+138
+00:10:32,890 --> 00:10:36,930
+فبالتالي لازم يكون موجود في الميديا السترش أجار
+
+139
+00:10:36,930 --> 00:10:41,490
+ممكن تيجي جاهزة ببوتل وممكن أنا أحضرها كيف بحضرها
+
+140
+00:10:41,490 --> 00:10:47,170
+بجيب ال nutrient أجار و بضيف عليها استرش since no
+
+141
+00:10:47,170 --> 00:10:50,210
+color change occur in the media when organism
+
+142
+00:10:50,210 --> 00:10:54,550
+hydrolyzes starch we added we add iodine to the
+
+143
+00:10:54,550 --> 00:10:58,950
+blood after incubation iodine turns blue purpleor
+
+144
+00:10:58,950 --> 00:11:02,370
+black dependent on the concentration of iodine in
+
+145
+00:11:02,370 --> 00:11:06,370
+the presence of starch عشان نفهم شو قصده بهذه
+
+146
+00:11:06,370 --> 00:11:11,110
+الفقرة خلينا نشرح ال procedure هذه الفقرة هي ال
+
+147
+00:11:11,110 --> 00:11:15,990
+principle الفحص من هنا هي ال principle الفحص لكن
+
+148
+00:11:15,990 --> 00:11:18,470
+خلينا نحكي عن ال procedure
+
+149
+00:11:26,190 --> 00:11:30,630
+البروسيجر بقولي انه انا بحضر ال starch agar طبعا
+
+150
+00:11:30,630 --> 00:11:34,470
+زي خطوات تحضير ال media اللي اخدناها بعد ما احضر
+
+151
+00:11:34,470 --> 00:11:38,310
+ال starch agar بزرع البكتيريا اللي بدي افحص قدرتها
+
+152
+00:11:38,310 --> 00:11:42,590
+على انتاج هذا الانزين كيف طريقة الزراعة بزرع على
+
+153
+00:11:42,590 --> 00:11:47,170
+شكل خط يبقى بقولي extract the test organism across
+
+154
+00:11:47,170 --> 00:11:53,020
+a sample portion of the agar surface امن الزراعالـ
+
+155
+00:11:53,020 --> 00:11:56,000
+organism اللى انا بدي افحص قدرته على انتاج هذا
+
+156
+00:11:56,000 --> 00:12:01,440
+الانزيم بشكل خطب باخد الانيكولام بعد ما انا زرعت
+
+157
+00:12:01,440 --> 00:12:06,320
+طبعا بحط هذا الطبق اللى انا زرعته فى ال incubator
+
+158
+00:12:06,320 --> 00:12:14,020
+على درجة حرارة 37 لمدة 48 ساعة او 24 بعد ال
+
+159
+00:12:14,020 --> 00:12:19,480
+incubation طبعا هلاقي انا عندى نمو للبكتيريا زرعت
+
+160
+00:12:19,480 --> 00:12:23,460
+البكتيريا نمت البكتيرياإذا كان عندها انزيم
+
+161
+00:12:23,460 --> 00:12:27,180
+الاميلاز هتكون كسرت ال starch وإذا ما كان عندها
+
+162
+00:12:27,180 --> 00:12:31,640
+الانزيم مش هتكون كسرت ال starch طيب كيف بدي أكشف
+
+163
+00:12:31,640 --> 00:12:37,340
+عن هال إشي بروح أنا بضيف indicator أو مادة تتفاعل
+
+164
+00:12:37,340 --> 00:12:41,980
+معها ال starch وتعطي لون معين لو البكتيريا عندها
+
+165
+00:12:41,980 --> 00:12:46,420
+انزيم الاميلاز هتكسر ال starch يعني ال starch مش
+
+166
+00:12:46,420 --> 00:12:51,240
+موجود يعني مش هيصير تفاعل وما رح يعطي اللون المعين
+
+167
+00:12:51,890 --> 00:12:56,770
+لكن لو البكتيريا ما عندها ال enzyme ال starch ما
+
+168
+00:12:56,770 --> 00:13:01,050
+رح يتكسر هيظل موجود في ال media يعني هيصير التفاعل
+
+169
+00:13:01,050 --> 00:13:06,210
+يعطي لون معين هذا هو ال principle الفحص طيب شو هو
+
+170
+00:13:06,210 --> 00:13:10,510
+ال indicator اللي احنا بنضيفه بقولنا بتضيفي iodine
+
+171
+00:13:10,510 --> 00:13:14,470
+ال iodine أخدناها في ال gram stain نفسه اللي احنا
+
+172
+00:13:14,470 --> 00:13:18,880
+استخدمناه في ال gram stain بقولنا بعدالـ
+
+173
+00:13:18,880 --> 00:13:22,980
+Incubation لمدة 24 ساعة وبعد ما تنمو البكتيريا
+
+174
+00:13:22,980 --> 00:13:28,540
+بغمر الطبق بال Iodine بغمر كل الطبق بال Iodine
+
+175
+00:13:28,540 --> 00:13:33,980
+وبشوف نتيجة التفاعل Iodine will turn blue when it
+
+176
+00:13:33,980 --> 00:13:38,800
+reacts with starch بقوللي ال Iodine بتحول للون
+
+177
+00:13:38,800 --> 00:13:43,170
+الأزرق لو اتفاعل مع ال starchA clear zone will be
+
+178
+00:13:43,170 --> 00:13:47,290
+seen where starch has been digested لو السترش
+
+179
+00:13:47,290 --> 00:13:52,450
+اتكسر بقولي النتيجة بتكون clear zone يبقى اللون ال
+
+180
+00:13:52,450 --> 00:13:56,210
+blue لو كانت النتيجة negative لكن لو كانت النتيجة
+
+181
+00:13:56,210 --> 00:14:00,270
+positive موجود الأميلاز بتكون clear zone ال
+
+182
+00:14:00,270 --> 00:14:05,770
+principle الفحص لسترش لو كانت البكتيريا ما عندها
+
+183
+00:14:05,770 --> 00:14:11,740
+أنزيم الأميلازي بقى كانت negative للأميلازماتكسر
+
+184
+00:14:11,740 --> 00:14:16,720
+الاسترش دلو موجود ضفت الايوداين الايوداين اتفاعل
+
+185
+00:14:16,720 --> 00:14:20,420
+مع الاسترش واعطاني لون blue او purple او black
+
+186
+00:14:20,420 --> 00:14:23,940
+طبعا بيعطيني اللون اي لون من هذه الألوان حسب ال
+
+187
+00:14:23,940 --> 00:14:29,180
+concentration of الايوداين لو كانت البكتيريا عندها
+
+188
+00:14:29,180 --> 00:14:32,580
+انزيم الاميلازي كانت positive للاميلازي يبقى
+
+189
+00:14:32,580 --> 00:14:36,990
+الاسترش مش هيكون موجودلما أضيف ال iodine مش هيصير
+
+190
+00:14:36,990 --> 00:14:40,410
+في عندي لون فبالتالي بقولي هيكون في عندي clear
+
+191
+00:14:40,410 --> 00:14:48,470
+zone حوالين البكتيريا هذا بالنسبة لل principle هذا
+
+192
+00:14:48,470 --> 00:14:53,410
+الطبق بعد ما انا طلعته من ال incubator بعد ما نمت
+
+193
+00:14:53,410 --> 00:14:57,970
+البكتيريا هذا الطبق بعد ما انا ضفت ال iodine تمام
+
+194
+00:14:57,970 --> 00:15:02,960
+بقولي انهبعد ما اناضفت ال iodine في زراعة بكتيريا
+
+195
+00:15:02,960 --> 00:15:07,940
+X و بكتيريا Y نلاحظ انه بكتيريا X هانتكون ال clear
+
+196
+00:15:07,940 --> 00:15:12,420
+zone قولنا ال clear zone معناه انه ال starch سله
+
+197
+00:15:12,420 --> 00:15:16,440
+هيدروليسز اتكسر يبقى ال starch مش موجود لما ييجي
+
+198
+00:15:16,440 --> 00:15:20,060
+ال iodine يرتبط فيه مش هيكون فيه تفاعل فهيكون فيه
+
+199
+00:15:20,060 --> 00:15:25,760
+clear zone يبقى النتيجة هاي هي positive لكن هان
+
+200
+00:15:25,760 --> 00:15:31,210
+النتيجة هي negativeهنا الاسترش ما اتكسر الاسترش
+
+201
+00:15:31,210 --> 00:15:35,010
+دلو موجود ايجا ال iodine ارتبط معاه اعطاني لون
+
+202
+00:15:35,010 --> 00:15:40,210
+blue او purple او black حسب ال concentration of
+
+203
+00:15:40,210 --> 00:15:43,350
+iodine لكن احنا في المعمل ان شاء الله هيكون اللون
+
+204
+00:15:43,350 --> 00:15:48,230
+black بقولي من الأمثلة على ال amylase positive
+
+205
+00:15:48,230 --> 00:15:51,750
+اللي هي البصيلة ال subcules هي موجودة على الشمال
+
+206
+00:15:52,070 --> 00:15:55,990
+لكن الـ E.coli موجودة على اليمين البصيلة الـ
+
+207
+00:15:55,990 --> 00:15:59,290
+Subtules هي positive للأميلاز ملاحظ في عندي clear
+
+208
+00:15:59,290 --> 00:16:04,350
+zone around growth لكن هنا في عندي اللون اللي هو
+
+209
+00:16:04,350 --> 00:16:09,590
+مش واضح كتير لكن بيكون اللون black يبقى البصيلة
+
+210
+00:16:09,590 --> 00:16:13,770
+الـ Subtules مثال على الأميلاز positive لكن الـ E
+
+211
+00:16:13,770 --> 00:16:20,180
+.coli مثال على الأميلاز negativeأحنا قلنا في أول
+
+212
+00:16:20,180 --> 00:16:24,900
+ال .. أول slide أنه الـ starch agar تعتبر
+
+213
+00:16:24,900 --> 00:16:28,700
+differential media بتميز بكتيريا عن بكتيريا وهذا
+
+214
+00:16:28,700 --> 00:16:32,020
+اللي اللي أحنا لاحظناه في ال result ميازات لي
+
+215
+00:16:32,020 --> 00:16:37,560
+أعطتني في البصل لسه بتيوز لون clear zone لكن في ال
+
+216
+00:16:37,560 --> 00:16:41,500
+E.coli أعطتني لون تاني فقدرت أميز بكتيريا عن
+
+217
+00:16:41,500 --> 00:16:42,440
+بكتيريا
+
+218
+00:16:44,800 --> 00:16:48,480
+بالنسبة للأميلاز النتائج إذا كانت عندها positive
+
+219
+00:16:48,480 --> 00:16:51,620
+إذا كان عندها ال enzyme يبقى بيكون في عندى clear
+
+220
+00:16:51,620 --> 00:16:55,420
+zone of hydrolysis مثال عليها البصلة ال subcules
+
+221
+00:16:55,420 --> 00:16:59,800
+لكن لو كانت negative للأميلاز بيكون اللون blue
+
+222
+00:16:59,800 --> 00:17:03,300
+purple or black حسب ال concentration of iodine
+
+223
+00:17:03,300 --> 00:17:07,200
+مثال عليها ال E coli هذه الصورة هي في عندى clear
+
+224
+00:17:07,200 --> 00:17:11,920
+zone حوالين النمو يبقى هيالنتيجة positive هاي بصل
+
+225
+00:17:11,920 --> 00:17:16,720
+ل subcules لكن هان بلاحظ ان في عندي اللون أسود
+
+226
+00:17:16,720 --> 00:17:22,280
+حوالين البكتيريا أو حوالين النمو يبقى هي ال E.coli
+
+227
+00:17:22,280 --> 00:17:27,700
+طبعا بلاحظ هان ان هاي المناطق ماوصلت إليها
+
+228
+00:17:27,700 --> 00:17:32,780
+البكتيريا فبالتالي هيظل ال starch موجود هيرتبط بال
+
+229
+00:17:32,780 --> 00:17:34,680
+أيضاين ويعطيين اللون ال black
+
+230
+00:17:38,890 --> 00:17:43,370
+في الـ slide اللي قبل كان ذاكر أنه البصيلة الـ sub
+
+231
+00:17:43,370 --> 00:17:47,230
+-tools البصيلة
+
+232
+00:17:47,230 --> 00:17:50,470
+الـ sub-tools هي positive للبحث و للفحص و الـ
+
+233
+00:17:50,470 --> 00:17:54,990
+Equality negative للفحص لكن ما بحكي أن هذا يعتبر
+
+234
+00:17:54,990 --> 00:17:59,460
+significantهذا مش significant فقط لكن مثال على الـ
+
+235
+00:17:59,460 --> 00:18:02,740
+positive و الـ negative للفحص لأن الـ E.coli جرام
+
+236
+00:18:02,740 --> 00:18:06,100
+نيجاتيف بصيلاي و البصيلة السابتيولز تعتبر جرام
+
+237
+00:18:06,100 --> 00:18:09,480
+positive بصيلاي يبقى من الأصل من الجرامستين بقدر
+
+238
+00:18:09,480 --> 00:18:13,500
+أني أنا أفرق بينهم غير كده أن الـ E.coli تعتبر non
+
+239
+00:18:13,500 --> 00:18:17,360
+spar farming البصيلة السابتيولز تعتبر spar farming
+
+240
+00:18:17,360 --> 00:18:22,600
+لو بدنا نفرق الاميلاز positive للبصيلة السابتيولز
+
+241
+00:18:22,600 --> 00:18:28,010
+و ال E.coli اللي هي الاميلاز نيجاتيفهك منكون خلصنا
+
+242
+00:18:28,010 --> 00:18:33,570
+أول فحص اللى هو الـomyelase هنتقل للجيلاتيناز
+
+243
+00:18:33,570 --> 00:18:40,910
+هنتكلم
+
+244
+00:18:40,910 --> 00:18:44,830
+عن تانى أنزايم اللى هو الجيلاتيناز الجيلاتيناز
+
+245
+00:18:44,830 --> 00:18:48,990
+يعتبر exo enzyme ال substrate اللى هيشتغل عليها
+
+246
+00:18:48,990 --> 00:18:53,920
+الجيلاتينقلنا ان الجيلاتين يعتبر protein high
+
+247
+00:18:53,920 --> 00:18:58,600
+molecular weight لازم الانزيم يطلع لبرا ويكسره ل
+
+248
+00:18:58,600 --> 00:19:03,320
+low molecular weight هيكسره ل amino acid ال amino
+
+249
+00:19:03,320 --> 00:19:06,740
+acid هي سوليوبول بتقدر تدخل لل cell membrane
+
+250
+00:19:07,210 --> 00:19:12,050
+الأمينو أسد تعتبر كمصدر للنيتروجين نيتروجين source
+
+251
+00:19:12,050 --> 00:19:15,790
+تستخدم في ال synthesis of structural and
+
+252
+00:19:15,790 --> 00:19:20,950
+functional cellular protein اللازم لنمو البكتيريا
+
+253
+00:19:20,950 --> 00:19:24,050
+الجيلاتين
+
+254
+00:19:24,050 --> 00:19:28,550
+يعتبر بروتين مشتق من الكلجن الموجود في ال
+
+255
+00:19:28,550 --> 00:19:34,010
+connective tissue كانوا زمان يعتبروا الجيلاتينكـ
+
+256
+00:19:34,010 --> 00:19:38,590
+solidifying agent لكن في أنواع من البكتيريا بتفرز
+
+257
+00:19:38,590 --> 00:19:43,450
+أنزيم الجيلاتيناز بيكسر الجيلاتين وبتحول الوسط من
+
+258
+00:19:43,450 --> 00:19:49,630
+solid ل liquid وهي فكرة الكشف عن الانزيم ومعرفة هل
+
+259
+00:19:49,630 --> 00:19:54,210
+البكتيريا عندها انزيم الجيلاتيناز او لأ الفكرة انه
+
+260
+00:19:54,210 --> 00:19:59,010
+بيكون عندى في ال medium جيلاتين كsubstrate
+
+261
+00:19:59,710 --> 00:20:03,590
+الجيلاتينياز طبعا في البكتيريا هاكشف هل البكتيريا
+
+262
+00:20:03,590 --> 00:20:07,290
+عندها هذا الانزيم ولا لأ هو exo enzyme لو
+
+263
+00:20:07,290 --> 00:20:11,290
+البكتيريا عندها انزيم الجيلاتينياز هتكسر الجيلاتين
+
+264
+00:20:11,290 --> 00:20:15,670
+هتحوله ل amino acid زائد small peptide يبقى
+
+265
+00:20:15,670 --> 00:20:19,710
+الجيلاتين كان هو يعتبر solidifying agent هو اللي
+
+266
+00:20:19,710 --> 00:20:23,910
+خلّى ال media صلبة لما اتكسر الجيلاتين صار الوسط
+
+267
+00:20:23,910 --> 00:20:28,730
+سائل liquid يبقى هذا ال principle الفحصإنها الـ
+
+268
+00:20:28,730 --> 00:20:38,430
+media بعد ما كانت صلبة هستير سائلة Determine
+
+269
+00:20:38,430 --> 00:20:42,430
+an organism ability to produce proteolytic-like
+
+270
+00:20:42,430 --> 00:20:46,510
+enzyme and lycophygelatin فقوللي هذا الفحص عشان
+
+271
+00:20:46,510 --> 00:20:51,510
+أعرف هل البكتيريا بتفرز أنزيماتبتعمل البروتيوليتك
+
+272
+00:20:51,510 --> 00:20:56,290
+بتحلل البروتينات وبتخلي الوساط سائل البروتيوليتك
+
+273
+00:20:56,290 --> 00:21:00,430
+يعني license of a protein this test is used to
+
+274
+00:21:00,430 --> 00:21:05,210
+differentiate gram negative species هذه الفقرة هي
+
+275
+00:21:05,210 --> 00:21:08,850
+ال significant احنا قلنا كل فحص فيه له significant
+
+276
+00:21:08,850 --> 00:21:13,050
+مين ال positive و ال negative او بيفرق مين عن مين
+
+277
+00:21:13,050 --> 00:21:16,730
+وليها ده يستخدم to differentiate gram negative
+
+278
+00:21:16,730 --> 00:21:23,770
+speciesCeracea, Sodomonas and Vibrio هدول كلهم
+
+279
+00:21:23,770 --> 00:21:27,590
+positive لفحص الجيلاتين عندهم جيلاتينيز بيكسر
+
+280
+00:21:27,590 --> 00:21:31,150
+الجيلاتين ال test can be used to differentiate
+
+281
+00:21:31,150 --> 00:21:36,390
+genera of gelatinase producing bacteria such as
+
+282
+00:21:36,390 --> 00:21:40,290
+Ceracea and Brutias from other members of the
+
+283
+00:21:40,290 --> 00:21:45,090
+family Enterobacteriaceae بقوللي أن هذا الفحص
+
+284
+00:21:45,090 --> 00:21:49,720
+هيميزالبروتياس والسيراشيا هدول يعتبروا positive
+
+285
+00:21:49,720 --> 00:21:54,180
+للجيلاتيناز عن باقي أعضاء ال انتيرو بكترياس
+
+286
+00:21:54,180 --> 00:21:58,360
+الانتيرو بكترياس عائلة بتضم أكتر من بكتيريا من
+
+287
+00:21:58,360 --> 00:22:03,280
+ضمنها السيراشيا والبروتياس هدول هما فقط ال
+
+288
+00:22:03,280 --> 00:22:06,680
+positive لهذا الفحص من عائلة الانتيرو بكترياس
+
+289
+00:22:06,680 --> 00:22:11,280
+فبالتالي ممكن أن أنا أفرق هدول عن باقي أعضاء
+
+290
+00:22:11,280 --> 00:22:16,890
+الانتيرو بكترياس بفحص الجيلاتينازبقول لي إنه الفحص
+
+291
+00:22:16,890 --> 00:22:20,710
+هذا ال issue العملي لهذا الفحص لحد الآن لم يقدر
+
+292
+00:22:20,710 --> 00:22:26,310
+وفي طرق للكشف عن الانزيم بشكل أسرع هنتعرف عليها
+
+293
+00:22:26,310 --> 00:22:31,370
+خلال المحاضرة Staphylococcus aureus which is
+
+294
+00:22:31,370 --> 00:22:34,750
+gelatinous positive can be differentiated from
+
+295
+00:22:34,750 --> 00:22:38,490
+Staphylococcus epidermidis gelatinous negative
+
+296
+00:22:39,500 --> 00:22:43,460
+الستافيلو كوكاس أورياس جيلتان جيلاتيناز positive
+
+297
+00:22:43,460 --> 00:22:47,240
+لكن الاستافيلو كوكاس ايه بدار مدرس تعتبر جيلاتيناز
+
+298
+00:22:47,240 --> 00:22:52,140
+negative احنا قلنا انه الاستافيلو كوكاي بشكل عام
+
+299
+00:22:52,140 --> 00:22:58,260
+الجنراهاي gram positive كوكاي كتلاز positive تمام
+
+300
+00:22:58,570 --> 00:23:02,350
+الستفيلوكوكاي كتلاز positive عشان افرق ال species
+
+301
+00:23:02,350 --> 00:23:07,190
+عن بعض ذكرنا فحص كيميائي اللي هو ال coagulase
+
+302
+00:23:07,190 --> 00:23:11,110
+قولنا انه الستفيلوكوكاس اورياس coagulase positive
+
+303
+00:23:11,110 --> 00:23:14,490
+لكن الستفيلوكوكاس ابيديرمتاس coagulase negative
+
+304
+00:23:14,490 --> 00:23:18,230
+فحص تاني اللي هو الجيلاتيناز نفس الاشي
+
+305
+00:23:18,230 --> 00:23:22,430
+الستفيلوكوكاس اورياس جيلاتيناز positive لكن
+
+306
+00:23:22,430 --> 00:23:26,610
+الستفيلوكوكاس ابيديرمتاس جيلاتيناز negativeالـ
+
+307
+00:23:26,610 --> 00:23:29,930
+Staphylococcus aureus قلنا هي pathogenic لكن الـ
+
+308
+00:23:29,930 --> 00:23:33,550
+Staphylococcus epidermidis هي abortionistic في
+
+309
+00:23:33,550 --> 00:23:39,670
+كمان فحصين هيمروا معنا خلال الشريح هذا هو المقارنة
+
+310
+00:23:39,670 --> 00:23:42,890
+هما تنتيني ground positive ككاي كتلز positive لـ
+
+311
+00:23:42,890 --> 00:23:45,650
+Staphylococcus aureus لكن في end species لـ
+
+312
+00:23:45,650 --> 00:23:47,850
+Staphylococcus aureus و لـ Staphylococcus
+
+313
+00:23:47,850 --> 00:23:50,590
+epidermidis قلنا الـ Coagulase positive لـ
+
+314
+00:23:50,590 --> 00:23:54,050
+Staphylococcus aureus و الجيلاتيناز positiveفي
+
+315
+00:23:54,050 --> 00:23:57,190
+عندي فحصين هنتعرف عليهم أو هيمروا معانا خلال
+
+316
+00:23:57,190 --> 00:24:01,150
+الشباتر الجاي ال money toll و ال DNS برضه positive
+
+317
+00:24:01,150 --> 00:24:04,370
+في الاستفالة الكوكس او yes لكن negative في
+
+318
+00:24:04,370 --> 00:24:06,190
+الاستفالة الكوكس epidermis
+
+319
+00:24:09,210 --> 00:24:12,990
+Bacillus anthrax and Bacillus cereus and several
+
+320
+00:24:12,990 --> 00:24:17,570
+other members of the genus are gelatinous positive
+
+321
+00:24:17,570 --> 00:24:21,250
+as are Clostridium titani and Clostridium
+
+322
+00:24:21,250 --> 00:24:24,750
+perifringus قلنا إن ال Clostridium و البacillus
+
+323
+00:24:24,750 --> 00:24:29,570
+يعتبروا إجرام positive بسلائي Spore forming بفرق
+
+324
+00:24:29,570 --> 00:24:34,120
+بينهم بفحص الكتلازالبصيلة الـ Sirius والبصيلة
+
+325
+00:24:34,120 --> 00:24:38,740
+الانتراكس بقولهم جيلاتيناز positive وكمان التنتان
+
+326
+00:24:38,740 --> 00:24:43,420
+إذا بدنا نربط المعلومات كانوا يعتبروا central in
+
+327
+00:24:43,420 --> 00:24:47,500
+the endospar الكلستريديوم تيتانيب والكلستريديوم
+
+328
+00:24:47,500 --> 00:24:51,420
+بيرفرنجز برضه يعتبروا جيلاتيناز positive يبقى هذا
+
+329
+00:24:51,420 --> 00:24:54,440
+هو ال significant للفحص
+
+330
+00:24:57,230 --> 00:25:01,310
+Gelatin is a protein بقولّي هو عبارة عن بروتين
+
+331
+00:25:01,310 --> 00:25:06,270
+derived from collagen هو عبارة عن بروتين مشتاق من
+
+332
+00:25:06,270 --> 00:25:09,610
+الـ collagen الموجود في الـ connective tissue
+
+333
+00:25:09,610 --> 00:25:14,090
+Gelatinase comprise a family of extracellular
+
+334
+00:25:14,090 --> 00:25:18,070
+enzyme قلنا جيلاتيناز يعتبر من الـ exoenzyme
+
+335
+00:25:18,070 --> 00:25:22,610
+produced and secreted by some microorganism قلنا
+
+336
+00:25:22,610 --> 00:25:26,760
+بيتم إنتاجهبواسطة بعض أنواع البكتيريا مش كل
+
+337
+00:25:26,760 --> 00:25:30,540
+البكتيريا عندها أنزيم الجيلاتيناز to hydrolyze
+
+338
+00:25:30,540 --> 00:25:34,800
+الجيلاتين عشان يتكسر الجيلاتين ويتحول ل amino acid
+
+339
+00:25:34,800 --> 00:25:39,340
+هذا ال amino acid يعتبر nitrogen source يستخدم في
+
+340
+00:25:39,340 --> 00:25:44,340
+إنتاج البروتين جيلاتين is a simple protein اتفقنا
+
+341
+00:25:44,340 --> 00:25:48,680
+هو عبارة عن simple protein هذه المعلومة مهمة جدا
+
+342
+00:25:48,680 --> 00:25:52,460
+بقول لي أن الجيلاتين بكون solid لما تكون درجة
+
+343
+00:25:52,460 --> 00:25:58,220
+الحرارةخمسة و عشرين درجة سليزيوسية او اقل بيكون
+
+344
+00:25:58,220 --> 00:26:01,700
+liquid لما تكون درجة الحرارة ستة و عشرين درجة
+
+345
+00:26:01,700 --> 00:26:07,020
+سليزيوس او اعلى يبقى الجيلاتين بتتغير حالته حسب
+
+346
+00:26:07,020 --> 00:26:11,380
+درجة الحرارة وهي معلومة مهمة جدا و ال bacteria
+
+347
+00:26:11,380 --> 00:26:15,060
+that produce the enzyme gelatinase are grown in a
+
+348
+00:26:15,060 --> 00:26:19,570
+gelatin media the enzyme break upThe gelatin
+
+349
+00:26:19,570 --> 00:26:23,390
+molecule and the medium cannot solidify even at
+
+350
+00:26:23,390 --> 00:26:27,590
+cold temperature هذه الجملة أو الفقرة هي ال
+
+351
+00:26:27,590 --> 00:26:31,050
+principle للفحص بولي أنا لو نميت البكتيريا اللي
+
+352
+00:26:31,050 --> 00:26:35,770
+عندها ال exoenzyme الجيلاتينايز في وسط فيه جيلاتين
+
+353
+00:26:35,770 --> 00:26:39,800
+اللي هو ال substrateأكيد الانزيم الجيلاتيناز
+
+354
+00:26:39,800 --> 00:26:43,780
+الموجود في البكتيريا هيشتغل على ال substrate ال
+
+355
+00:26:43,780 --> 00:26:46,960
+substrate اللي هي الجيلاتين الموجود في ال media
+
+356
+00:26:46,960 --> 00:26:51,400
+هيكسر الجيلاتين اللي هو solid هيحوله ل amino acid
+
+357
+00:26:51,400 --> 00:26:56,440
+زائد small peptide هيبطل الوسط صلب هيصير الوسط
+
+358
+00:26:56,440 --> 00:27:00,320
+سائل يبقى ال media مش هتقدر تتصلب حتى في ال cold
+
+359
+00:27:00,320 --> 00:27:04,360
+temperature طب ليش قاللي حتى في ال cold
+
+360
+00:27:04,360 --> 00:27:04,980
+temperature
+
+361
+00:27:07,390 --> 00:27:11,090
+لأنه بالجملة اللى قبلها قالنا ان الجيلاتين بكون
+
+362
+00:27:11,090 --> 00:27:16,630
+صلب في درجات الحرارة المنخفضة عند 25 درجة سيليزيا
+
+363
+00:27:16,630 --> 00:27:21,650
+او اقل يعني بكون صلب في الكوال تمريتشار طيب بما
+
+364
+00:27:21,650 --> 00:27:25,330
+انه اتكسر الجيلاتين بطل عندى جيلاتين صار amino
+
+365
+00:27:25,330 --> 00:27:29,730
+acid يبقى مش هتتصلى بال media حتى لو كانت درجة
+
+366
+00:27:29,730 --> 00:27:33,770
+الحرارة منخفضة two methods used to determine
+
+367
+00:27:33,770 --> 00:27:37,940
+gelatinase production بقولى فيه عندىطريقتين لكشف
+
+368
+00:27:37,940 --> 00:27:41,640
+على ال gelatinous production طريقة الأولى gelatin
+
+369
+00:27:41,640 --> 00:27:45,540
+stab method الطريقة التانية gelatin strip method
+
+370
+00:27:45,540 --> 00:27:53,860
+من نضيف طريقة تالتة وهي le charcoal طريقة
+
+371
+00:27:53,860 --> 00:27:58,060
+تالتة وهي اسمها le charcoal method
+
+372
+00:28:10,660 --> 00:28:19,700
+التشاركول method يبقى
+
+373
+00:28:19,700 --> 00:28:22,440
+الطريقة الأولى الجيلاتين step method الجيلاتين
+
+374
+00:28:22,440 --> 00:28:26,220
+strip method التشاركول method هنتعرف على كل طريقة
+
+375
+00:28:26,220 --> 00:28:30,040
+شو ال principle اللي لها شو طريقة الشغل وكيف بتكون
+
+376
+00:28:30,040 --> 00:28:30,580
+ال result
+
+377
+00:28:35,040 --> 00:28:38,640
+في هاي ال slide ذاكر Sirachia Sodomonas Vibrio
+
+378
+00:28:38,640 --> 00:28:45,120
+كلهم يعتبروا جيلاتيناز positive أول طريقة عشان
+
+379
+00:28:45,120 --> 00:28:48,960
+أكشف على الجيلاتيناز هي ال gelatin stab method
+
+380
+00:28:48,960 --> 00:28:54,740
+الطريقة هي أني أنا بجيب التيوب طبعا مكتوب عندي أنا
+
+381
+00:28:54,740 --> 00:28:59,540
+أستابي بدي أعمل stabbing طاعن هتكون في التيوب
+
+382
+00:28:59,710 --> 00:29:04,870
+Hyalite-U بتحتوي على nutrient بروث البروث وليس
+
+383
+00:29:04,870 --> 00:29:09,990
+أجار ضروري جدا أن تكون البروث مش أجار لأن الأجار
+
+384
+00:29:09,990 --> 00:29:14,250
+يعتبر solidifying agent هتظلها صليت سواء كانت
+
+385
+00:29:14,250 --> 00:29:18,670
+البكتيريا positive أو negative للجيلاتيناز يبقى
+
+386
+00:29:18,670 --> 00:29:24,030
+ممنوع أحط أجار لأن لو حطت أجار ال media هتظل صليت
+
+387
+00:29:24,030 --> 00:29:26,230
+سواء كانت البكتيريا positive أو negative
+
+388
+00:29:26,230 --> 00:29:33,710
+للجيلاتينازهضيف 12% جيلاتين يبقى الـ 12% الجيلاتين
+
+389
+00:29:33,710 --> 00:29:38,910
+هيكون كsubstrate كيف بدى انا احضر هذه ال media
+
+390
+00:29:38,910 --> 00:29:44,250
+بقوللي بتجيبي ال nutrient broth تمام تقرا ايه شو
+
+391
+00:29:44,250 --> 00:29:48,270
+التعليمات الموجودة على البطولة كنت كتبلي انه كل
+
+392
+00:29:48,270 --> 00:29:52,190
+1000 ملي ضيف عليها 13 جرام من ال nutrient broth طب
+
+393
+00:29:52,190 --> 00:29:56,860
+انا بدى احضر 500 مل بعمل ضرب تبادلىبيطلع ان انا
+
+394
+00:29:56,860 --> 00:30:00,860
+بدي 6.5 جرام من الـ nutrient broth طيب نسبة
+
+395
+00:30:00,860 --> 00:30:06,400
+الجيلاتين بقوللي المستخدمة في هذا الفحص 12% 12%
+
+396
+00:30:06,400 --> 00:30:11,900
+يعني 12 لكل 100 مل دستيل ووتر طب انا اضيفت 500 مل
+
+397
+00:30:11,900 --> 00:30:16,220
+بعمل درد تبادل يبقى بدي 60 جرام of جيلاتين يبقى
+
+398
+00:30:16,220 --> 00:30:20,540
+بضيف ال 60 جرام من الجيلاتين على 6.5 جرام من ال
+
+399
+00:30:20,540 --> 00:30:25,720
+nutrient brothبعملهم mix فيه 500 مل دستل واتر على
+
+400
+00:30:25,720 --> 00:30:30,380
+الـ hot plate بعملهم mix بحطهم على ال hot plate
+
+401
+00:30:30,380 --> 00:30:35,480
+بعد ما يدوبوا منيح بحطهم فيه تيوب بحط سواب بدخلهم
+
+402
+00:30:35,480 --> 00:30:40,040
+في ال auto clef ال auto clef هيعقم للميديا بستنها
+
+403
+00:30:40,040 --> 00:30:44,540
+تبرد وبعدين بشتغل الفحص لتيوب بعد ما تطلع بسميها
+
+404
+00:30:44,540 --> 00:30:51,200
+nutrient gelatin deep tubeتمام هذه بتحتوي على ال
+
+405
+00:30:51,200 --> 00:30:55,000
+substrate اللي هي الجيلاتين باجي باخد البكتيريا
+
+406
+00:30:55,000 --> 00:30:58,640
+اللي انا بدي اكشف عندها انزيم الجيلاتيناز او لأ
+
+407
+00:30:58,640 --> 00:31:02,020
+يبقى touch of بكتيريا من الطبق اللي انا بدي افحص
+
+408
+00:31:02,020 --> 00:31:04,320
+او البكتيريا اللي بدي افحصها لها انت الانزيم ولا
+
+409
+00:31:04,320 --> 00:31:07,280
+لأ يبقى عند ال substrate اللي موجود في ال media
+
+410
+00:31:07,280 --> 00:31:10,720
+اللي هو الجيلاتين بدي اكشف عن الانزيم اللي موجود
+
+411
+00:31:10,720 --> 00:31:14,760
+في البكتيريا او مش موجود لسه مش عارفة هعمل
+
+412
+00:31:14,760 --> 00:31:20,320
+stabbing هي هتكون deep زي فحص ال motilityهعقم
+
+413
+00:31:20,320 --> 00:31:24,540
+الفوقة و هدخل بشكل عمودي و هعمل stabbing هعمل طعنة
+
+414
+00:31:24,540 --> 00:31:29,240
+في النص تقريبا 3 تولتين و هطلع هعقم الفوقة هعمل
+
+415
+00:31:29,240 --> 00:31:37,080
+incubation لمدة أسبوع على 37 درجة سليسيا بعد ما
+
+416
+00:31:37,080 --> 00:31:42,600
+أعمل incubation بقوللي بحطيها في التلاجة لمدة 30
+
+417
+00:31:42,600 --> 00:31:47,660
+دقيقة هنا سؤال مهمليش انا بدي احطها في التلاجة
+
+418
+00:31:47,660 --> 00:31:53,520
+لمدة 30 دقيقة بقوللي لما طليها من ال incubation
+
+419
+00:31:53,520 --> 00:31:57,860
+سواء كان البكتيريا عندها أنزيم الجيلاتيناز او ما
+
+420
+00:31:57,860 --> 00:32:02,460
+عندها هتكون liquid ليش؟ لو كانت البكتيريا عندها
+
+421
+00:32:02,460 --> 00:32:06,700
+أنزيم الجيلاتيناز هتكون liquid لأنه الجيلاتيناز
+
+422
+00:32:06,700 --> 00:32:10,440
+كسر الجيلاتين اتحول الوسط من solid ل liquid حسب
+
+423
+00:32:10,440 --> 00:32:15,060
+المعادلة اللي ذكرناها في أول slide طيب
+
+424
+00:32:16,490 --> 00:32:22,630
+لو ما عندها انزيم الجيلاتيناز ليش ضلت او ليش كانت
+
+425
+00:32:22,630 --> 00:32:27,130
+liquid بقولي لو كانت ما عندها انزيم الجيلاتيناز
+
+426
+00:32:27,130 --> 00:32:32,930
+تمام الجيلاتين مش هيتكسر تمام هيضل الجيلاتين في ال
+
+427
+00:32:32,930 --> 00:32:37,550
+media لكن احنا قولنا في ال slide ان الجيلاتين في
+
+428
+00:32:37,550 --> 00:32:45,200
+درجات الحرارة لما يكون من 25 او اعلى بتكوناللي
+
+429
+00:32:45,200 --> 00:32:49,560
+بتكون حالته liquid طب درجة ال incubator حرارة ال
+
+430
+00:32:49,560 --> 00:32:54,360
+incubator 37 يبقى أعلى من 26 يبقى هيكون liquid
+
+431
+00:32:54,360 --> 00:32:59,880
+فبالتالي بقولنا بنحطها في التلاجة لمدة 30 دقيقة
+
+432
+00:32:59,880 --> 00:33:07,190
+عشان أعرف و أحدد هل ال liquid نتيجة تكسيرالانزيم
+
+433
+00:33:07,190 --> 00:33:11,110
+الجيلاتيناز للجيلاتين واتحول لـ liquid ولا لأنه
+
+434
+00:33:11,110 --> 00:33:16,070
+كان ال incubator درجة حرارته أعلى من 26 فالجيلاتين
+
+435
+00:33:16,070 --> 00:33:21,530
+صار في الحالة السائلة فاحطه في التلاجة لمدة 30
+
+436
+00:33:21,530 --> 00:33:26,390
+minute هشوف إذا ضلوا liquid يبقى النتيجة بتكون
+
+437
+00:33:26,390 --> 00:33:30,030
+positive فعندها انزيم الجيلاتيناز كسر الجيلاتين
+
+438
+00:33:30,030 --> 00:33:37,450
+أعطاني أمينو أسد اتحول الوسط ل liquid لكنلو اتحول
+
+439
+00:33:37,450 --> 00:33:42,890
+ل solid بقول لي ما بكتب negative اذا متذكرين فحص
+
+440
+00:33:42,890 --> 00:33:45,790
+الـ coagulase في ال tube method كنا نقول نفس
+
+441
+00:33:45,790 --> 00:33:50,870
+الفكرة بدي اعمل re incubation for total 30 days
+
+442
+00:33:50,870 --> 00:33:56,430
+بدي اعمل اكمل incubation اكمل ل 30 يوم احنا عملنا
+
+443
+00:33:56,430 --> 00:34:04,330
+أسبوع سبع أيام فانا بكمل 23 يوم incubation عشانفي
+
+444
+00:34:04,330 --> 00:34:08,910
+بعض أنواع البكتيريا بيكون عندها الانزيم بكميات
+
+445
+00:34:08,910 --> 00:34:14,050
+قليلة فبالتالي عشان تقدر تكسر الجيلاتين بياخد وقت
+
+446
+00:34:14,050 --> 00:34:18,610
+فأنا عشان أضمن أنه هذه البكتيريا لو كان في عندي
+
+447
+00:34:18,610 --> 00:34:21,750
+هذه البكتيريا بتفرز الانزيم بكميات قليلة عشان أضمن
+
+448
+00:34:21,750 --> 00:34:27,510
+هذا الاشي بخليها incubation لمدة 30 يومبعد ما يخلص
+
+449
+00:34:27,510 --> 00:34:32,570
+الـ 30 يوم بحطها في التلاجة لمدة 30 minute لنفس
+
+450
+00:34:32,570 --> 00:34:36,490
+السبب اللى ذكرناه قبل شوية لأنه سواء كانت positive
+
+451
+00:34:36,490 --> 00:34:39,870
+أو negative هتكون liquid لأنه درجة حرارة ال
+
+452
+00:34:39,870 --> 00:34:45,310
+incubator 37 لو كانت negative فبالتالي الجيلاتين
+
+453
+00:34:45,310 --> 00:34:48,550
+فيه لما تكون درجة الحرارة أعلى من 26 هيكون في
+
+454
+00:34:48,550 --> 00:34:52,610
+الحالة في ال liquid في الحالة السائلة لو كان عندها
+
+455
+00:34:52,610 --> 00:34:57,850
+ال enzyme أكيد هيتكسر فانا مش عارفةهل عند الانزيم
+
+456
+00:34:57,850 --> 00:35:04,810
+ولا لأ فأحطها في التلاجة لمدة 30 دقيقة إذا دلت
+
+457
+00:35:04,810 --> 00:35:08,930
+liquid يبقى هي positive لكن لو اتصلبت يبقى هي
+
+458
+00:35:08,930 --> 00:35:13,010
+negative نسجل النتيجة negative هذا بالنسبة
+
+459
+00:35:13,010 --> 00:35:17,110
+للجليلاتين step method الفكرة ال principle زي ما
+
+460
+00:35:17,110 --> 00:35:22,950
+قلنا انه الوسط بتتحول ل liquid هذا ال principle
+
+461
+00:35:23,500 --> 00:35:28,860
+وبالنسبة لـ Result إما Liquid أو Solid الـ Result
+
+462
+00:35:28,860 --> 00:35:33,720
+في الجيلاتين استعب ميثود لو كانت نتيجة Positive
+
+463
+00:35:33,720 --> 00:35:38,020
+بيكون Liquid بتنتج وينتهي عن أنظم الجيلاتيناز ممكن
+
+464
+00:35:38,020 --> 00:35:41,480
+يكون Strong Positive وممكن تكون Weak Positive
+
+465
+00:35:41,480 --> 00:35:46,040
+Strong Positive إنها بتتحول لـ Liquid خلال تلت
+
+466
+00:35:46,040 --> 00:35:50,840
+أياملكن لو كانت week positive خلال من تلت لتلتين
+
+467
+00:35:50,840 --> 00:35:54,420
+يوم بيصير عندى اللى هو بتحول الوسط ل liquid زى
+
+468
+00:35:54,420 --> 00:35:59,380
+البروتياز قولنا انه بيكون عندها الانزين الانزين
+
+469
+00:35:59,380 --> 00:36:03,220
+بكميات قليلة ال negative ان الوسط بيصير له
+
+470
+00:36:03,220 --> 00:36:07,460
+solidify بتصلب نتيجة ان الجيلاتين ما تكسر دلو
+
+471
+00:36:07,460 --> 00:36:12,640
+الجيلاتينمع تأنزيب الـ Gelatinase حتى بعد 30 يوم
+
+472
+00:36:12,640 --> 00:36:17,600
+بتظلها الـ media still solid هذا بالنسبة لنتائج
+
+473
+00:36:17,600 --> 00:36:24,080
+طريقة الـ Gelatin Stab Methodنمر على الـ slides في
+
+474
+00:36:24,080 --> 00:36:27,660
+الجيلاتين step method بقوللي انكيولات نيوتريان
+
+475
+00:36:27,660 --> 00:36:31,900
+جيلاتين ييب اتيوب بتحتوي على 12% جيلاتين بعملها
+
+476
+00:36:31,900 --> 00:36:36,740
+incubation لمدة أسبوع بحدد إذا صار في عندي
+
+477
+00:36:36,740 --> 00:36:40,880
+liquification أنه اتحول ال media ل liquid ولا لأ
+
+478
+00:36:40,880 --> 00:36:47,700
+أو بحطها في التلاجة لمدة 30 دقيقةبعدها بشوف بعد ما
+
+479
+00:36:47,700 --> 00:36:51,020
+اتخلص التلاتين دقيقة بتأكد انه صار في عندي
+
+480
+00:36:51,020 --> 00:36:54,600
+leakification ولا لأ بقول لو negative بتكملي
+
+481
+00:36:54,600 --> 00:36:59,140
+incubation لتلاتين يوم if the gelatin is still
+
+482
+00:36:59,140 --> 00:37:02,640
+intact لو الجيلاتين ضاله صلب يبقى ال bacteria
+
+483
+00:37:02,640 --> 00:37:06,540
+didn't produce gelatinase البكتيريا ما عندها أنزيم
+
+484
+00:37:06,540 --> 00:37:11,030
+الجيلاتيناز ال media will solidifyالـ media بتتصلب
+
+485
+00:37:11,030 --> 00:37:14,250
+في التلاجة و الـ negative result أنا بسجلها
+
+486
+00:37:14,250 --> 00:37:18,330
+negative result إنه لو أنا حاطيتها في التلاجة تمام
+
+487
+00:37:18,330 --> 00:37:24,350
+ال media هتتصلب لأنه درجة الحرارة أقل من 25 قولنا
+
+488
+00:37:24,350 --> 00:37:28,310
+إنه الجيلاتين بتغير حسب درجة الحرارة if the
+
+489
+00:37:28,310 --> 00:37:32,410
+organism has produced sufficient gelatinase لو كان
+
+490
+00:37:32,410 --> 00:37:36,970
+عند الorganism انزيم الجيلاتينازالتيوب سيبقى
+
+491
+00:37:36,970 --> 00:37:40,790
+مليكود حتى
+
+492
+00:37:40,790 --> 00:37:42,630
+لو انا حطيته في التلاجة
+
+493
+00:37:49,500 --> 00:37:53,920
+إذا بنلاحظ أنه أنا ميّلت فبت .. ميّلت ال .. التيوب
+
+494
+00:37:53,920 --> 00:37:58,960
+فالتيوب ما اتحرك يبقى ضاله الوسط solid يبقى هي
+
+495
+00:37:58,960 --> 00:38:03,260
+negative gelatinase لكنها ميّلت التيوب يبقى اتحرك
+
+496
+00:38:03,260 --> 00:38:08,920
+يبقى الوسط سائل يبقى هي positive gelatinase some
+
+497
+00:38:08,920 --> 00:38:13,150
+organism may produce gelatinasein rather small
+
+498
+00:38:13,150 --> 00:38:18,030
+quantities قلنا في بعض أنواع البكتيريا تفرز انزيم
+
+499
+00:38:18,030 --> 00:38:22,210
+الجيلاتيناز بكميات قليلة عشان هيك ال negative
+
+500
+00:38:22,210 --> 00:38:26,150
+gelatinase لازم اني انا عاملها re incubated for 30
+
+501
+00:38:26,150 --> 00:38:30,850
+days لمدة 30 يوم لازم اعمل incubation عشان اتأكد
+
+502
+00:38:30,850 --> 00:38:38,310
+انه هي negative result بالنسبة
+
+503
+00:38:38,310 --> 00:38:42,640
+لهذا ال slide قلنا السيراشياعبارة عن positive
+
+504
+00:38:42,640 --> 00:38:46,680
+للجيلاتين بقدر افرق بينها وبين باقي الـ
+
+505
+00:38:46,680 --> 00:38:50,400
+Enterobacteriasis سلمونيلا هي عبارة عن من عالة الـ
+
+506
+00:38:50,400 --> 00:38:52,720
+Enterobacteriasis قولنا احنا فقط من عالة الـ
+
+507
+00:38:52,720 --> 00:38:56,100
+Enterobacteriasis الجيلاتين positive السيراشيا و
+
+508
+00:38:56,100 --> 00:38:59,320
+البروتياز يبقى السلمونيلا هي بتكون negative
+
+509
+00:38:59,320 --> 00:39:04,040
+الميديا اتصلبت لكن هنا في السيراشيا هي positive
+
+510
+00:39:04,040 --> 00:39:08,520
+للجيلاتين بقولنا ان الميديا صارت liquid سائلة اذا
+
+511
+00:39:08,520 --> 00:39:13,600
+ملاحظها اليومفي عندي سائل اتحرك لطيوب لكنها صلبة
+
+512
+00:39:13,600 --> 00:39:20,480
+الميديا يبقى هي بتكون negative للجيلاتينيز النتائج
+
+513
+00:39:20,480 --> 00:39:23,660
+قلنا ممكن انها تكون positive strong او weak ال
+
+514
+00:39:23,660 --> 00:39:27,120
+strong خلال تلات أيام ال week من تلاتة لتلاتين يوم
+
+515
+00:39:27,120 --> 00:39:31,320
+ال negative حتى بعد تلاتين يوم ما بيصير في عندي
+
+516
+00:39:31,320 --> 00:39:34,360
+liquification بتظلها ال media solid
+
+517
+00:39:38,650 --> 00:39:42,750
+هذه الطريقة الأولى شرحناها نجلى الطريقة التانية
+
+518
+00:39:42,750 --> 00:39:45,910
+اللى هى ال gelatin strip method ال gelatin strip
+
+519
+00:39:45,910 --> 00:39:49,190
+method بقولنا بنجيب تيوب بنحط فيها bacterial
+
+520
+00:39:49,190 --> 00:39:51,670
+suspension اش يعنى bacterial suspension يعني
+
+521
+00:39:51,670 --> 00:39:54,690
+normal saline مع بكتيريا أو nutrient broth مع
+
+522
+00:39:54,690 --> 00:39:57,790
+بكتيريا طبعا البكتيريا اللى بدى اكشف عندها الانزيم
+
+523
+00:39:57,790 --> 00:40:02,450
+ولا لأ بقولنا بنضيف X-ray film ال X-ray film
+
+524
+00:40:02,450 --> 00:40:06,210
+بقولنا هى coated with green gelatin emulsion يبقى
+
+525
+00:40:06,210 --> 00:40:10,490
+هتكون هى ال substrateالـ X-ray film بتحتوي على
+
+526
+00:40:10,490 --> 00:40:13,370
+الجيلاتين يبقى هي السبسترات اللي هيشتغل عليها
+
+527
+00:40:13,370 --> 00:40:16,210
+الانزيم الموجود في البكتيريا او مش موجود انا بدي
+
+528
+00:40:16,210 --> 00:40:22,870
+اكشف عنه بعدها بعمل incubation على درجة 37 لمدة 4
+
+529
+00:40:22,870 --> 00:40:27,590
+ساعات لو البكتيريا عندها انزيم الجيلاتينازهتشتغل
+
+530
+00:40:27,590 --> 00:40:32,370
+على الجيلاتين الموجود بالـ X-ray و هتحاول انها ايش
+
+531
+00:40:32,370 --> 00:40:36,470
+تكسر هذا الجيلاتين فبالتالي اللون الأسود اللي
+
+532
+00:40:36,470 --> 00:40:42,250
+موجود على الفيلم لـ X-ray هيختفي و هيصير colorless
+
+533
+00:40:42,250 --> 00:40:46,010
+يبقى النتيجة ال positive انه الـ X-ray فيلم هيسله
+
+534
+00:40:46,010 --> 00:40:49,490
+دي سبير هيصير colorless لكن لو ضاله لونه أسود
+
+535
+00:40:49,490 --> 00:40:53,740
+بقوليماتسجل النتيجة negative بتعمل Re-incubation
+
+536
+00:40:53,740 --> 00:40:58,660
+for a total of 24 hours بتكمل الـ Incubation لـ 24
+
+537
+00:40:58,660 --> 00:41:02,420
+ساعة يبقى لما أنك احنا حطينا بالأول 4 ساعات بنعمل
+
+538
+00:41:02,420 --> 00:41:06,300
+الـ Incubation لمدة 20 ساعة لو دلوا لونه أسود يبقى
+
+539
+00:41:06,300 --> 00:41:12,020
+خلاص بسجل النتيجة negative لكن لو اتغير لـ الـ
+
+540
+00:41:12,020 --> 00:41:15,540
+Nesapir اختفى اللون الأسود وصار colorless يبقى هي
+
+541
+00:41:15,540 --> 00:41:20,070
+positive للجيلاتيناز لكن أخدت وقتلأنه اللي قلنا في
+
+542
+00:41:20,070 --> 00:41:24,850
+بعض أنواع البكتيريا بتفرز الانزيم بكميات قليلة هذا
+
+543
+00:41:24,850 --> 00:41:28,630
+بالنسبة لطريقة ال gelatin strip method هي
+
+544
+00:41:28,630 --> 00:41:31,710
+alternative method for detecting gelatinase
+
+545
+00:41:31,710 --> 00:41:35,190
+production طريقة تانية عشان أفحص عن الجيلاتيناز
+
+546
+00:41:35,190 --> 00:41:40,080
+باستخدم ال X-ray film ال gelatin stripبقولنا
+
+547
+00:41:40,080 --> 00:41:44,600
+الجيلاتين الـ X-ray film that is coated with a
+
+548
+00:41:44,600 --> 00:41:48,140
+green gelatin emulsion هي بتكون فيها اللي هو
+
+549
+00:41:48,140 --> 00:41:52,480
+الجيلاتين organism that produce gelatinase remove
+
+550
+00:41:52,480 --> 00:41:56,080
+بقولّي لو البكتيريا عندها أنزيم الجيلاتيناز هتزيل
+
+551
+00:41:56,080 --> 00:41:59,780
+ال emulsion from the strip هتزيل اللون الأسود من
+
+552
+00:41:59,780 --> 00:42:03,000
+ال strip كف الطريقة زي ما قلنا بعمل انكولات
+
+553
+00:42:03,000 --> 00:42:08,790
+للبكتيريا في نص مل من ال normal salineلازم يكون ال
+
+554
+00:42:08,790 --> 00:42:11,770
+suspension تربط بعدين بحط ال X-ray film أو
+
+555
+00:42:11,770 --> 00:42:16,330
+الجلاتين في ال bacterial suspension أو ال saline
+
+556
+00:42:16,330 --> 00:42:20,610
+suspension بعمل incubation لمدة أربع ساعات لأربع
+
+557
+00:42:20,610 --> 00:42:25,690
+عشرين ساعة عشان ألاحظ إزالة اللي هو الجلاتين
+
+558
+00:42:25,690 --> 00:42:34,070
+emulsion من ال strip من اللاحظ في هذه الصورةما
+
+559
+00:42:34,070 --> 00:42:38,650
+اختفى اللون الأسود من الـ Strip يبقى هذه النتيجة
+
+560
+00:42:38,650 --> 00:42:43,370
+Negative للجيلاتيناز لكن هنا نلاحظ أن الـ Strip
+
+561
+00:42:43,370 --> 00:42:47,370
+راح اللون الأسود منه يبقى هذه جيلاتيناز Positive
+
+562
+00:42:48,140 --> 00:42:52,180
+الجزء اللى فوق طبعا أكيد ما راح اللون لأنه ما صار
+
+563
+00:42:52,180 --> 00:42:55,980
+فيه touch بينه وبين البكتيريا البكتيريا موجودة في
+
+564
+00:42:55,980 --> 00:42:59,960
+ال suspension في السقل اللى تحت فأكيد اللى صار فيه
+
+565
+00:42:59,960 --> 00:43:03,400
+touch هو اللى هيروح هيصير التفاعل مع ال substrate
+
+566
+00:43:03,400 --> 00:43:06,540
+وهيروح اللون من ال strip يبقى high gelatinase
+
+567
+00:43:06,540 --> 00:43:11,710
+positive وhigh gelatinase negativeخلصنا طريقة اللى
+
+568
+00:43:11,710 --> 00:43:14,990
+هى الـ Gelatin Strip نجى لآخر طريقة اللى هى ال
+
+569
+00:43:14,990 --> 00:43:19,470
+charcoal method ال charcoal هو الفحم الفكرة ان
+
+570
+00:43:19,470 --> 00:43:25,070
+الفحم جزيئات ومتماسكة وسبب وجود الجيلاتين فيه يبقى
+
+571
+00:43:25,070 --> 00:43:27,890
+بجيب انا bacterial suspension normal saline مع
+
+572
+00:43:27,890 --> 00:43:31,270
+بكتيريا او nutrient broth مع بكتيريا البكتيريا بدي
+
+573
+00:43:31,270 --> 00:43:35,330
+اكشف موجود فيها الانزيم ولا لأ فجيب charcoal فحم و
+
+574
+00:43:35,330 --> 00:43:39,480
+احطه فيه high lithium بدل ال X-ray filmالقطش
+
+575
+00:43:39,480 --> 00:43:43,900
+charcoal هيكون في السبستريات تمام هعمل incubation
+
+576
+00:43:43,900 --> 00:43:49,100
+لمدة 24 ساعة على 37 درجة سيليزيا اذا لاحظت انه
+
+577
+00:43:49,100 --> 00:43:52,480
+الفحم اترسب في قاع التيوب يبقى النتيجة negative
+
+578
+00:43:52,480 --> 00:43:57,020
+لكن اذا لاحظت انه الفحم اتوزع في كل التيوب اتفكك
+
+579
+00:43:57,020 --> 00:44:01,730
+الفحم هيصير انتشار في كل تيوبهيتغير لون التيوب لأ
+
+580
+00:44:01,730 --> 00:44:06,350
+اللون الأسود يبقى نتيجة positive لأن البكتيريا
+
+581
+00:44:06,350 --> 00:44:10,370
+عندها انزيم الجيلاتيناز كسر الجيلاتين الجيلاتين هو
+
+582
+00:44:10,370 --> 00:44:14,130
+اللي بيخلّي الفحم متماسك طب الجيلاتين اتكسر راح
+
+583
+00:44:14,130 --> 00:44:17,910
+يبقى الفحم هينتشر في كل تيوب وهيصير لونه أسود يبقى
+
+584
+00:44:17,910 --> 00:44:21,710
+النتيجة ال positive distribution هيصير في يدي لون
+
+585
+00:44:21,710 --> 00:44:25,810
+أسود في كل تيوب لكن نتيجة ال negative مش هتفرز
+
+586
+00:44:25,810 --> 00:44:30,250
+انزيم الجيلاتيناز يبقى الجيلاتين هيضلهبين جزيئات
+
+587
+00:44:30,250 --> 00:44:34,790
+الفحم فبالتالي هيترسب في القاهة هذا فكرة let's
+
+588
+00:44:34,790 --> 00:44:41,410
+share cool هكذا خلصنا طحس الجيلاتيناز وخلصنا ال
+
+589
+00:44:41,410 --> 00:44:44,770
+chapter اللي كان بيتكلم عن الاميلاز and جيلاتيناز
+
+590
+00:44:44,770 --> 00:44:50,270
+وقلنا اتنين exoenzyme بتمنى تكون الأمور واضحة
+
+591
+00:44:50,270 --> 00:44:53,070
+والسلام عليكم ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/YWBUtjE9yHc_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/YWBUtjE9yHc_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..32a564dad717baf7e97872c0f5834984cc7a4d71
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/YWBUtjE9yHc_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 2343, "start": 2.09, "end": 23.43, "text": "السلام عليكم ورحمة الله وبركاته يعطيكم العافية جميعا في هذا اللقاء نستكمل سلسلة ال biochemical test في المحاضرة السابقة اتكلمنا على الانزيمات وقسمنا الانزيم حسب ال side of production ل endoenzyme و exoenzyme ال endoenzyme قلنا انه التفاعل بيصير intracellular داخل الخلية", "tokens": [6027, 3794, 37440, 25894, 24793, 4032, 2288, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224, 37495, 9566, 1829, 24793, 18863, 31845, 10632, 10874, 2304, 40228, 995, 8978, 23758, 13672, 4587, 16606, 8717, 14851, 24793, 1211, 8608, 1211, 3794, 37977, 2423, 12198, 34114, 1500, 8978, 9673, 5016, 46958, 25720, 21136, 16758, 28671, 1975, 2655, 4117, 19528, 8315, 15844, 2423, 7649, 11622, 32640, 9307, 4032, 4587, 38251, 8315, 2423, 7649, 11622, 32640, 11331, 35457, 2423, 1252, 295, 4265, 5296, 917, 78, 268, 1229, 1398, 4032, 454, 78, 268, 1229, 1398, 2423, 917, 78, 268, 1229, 1398, 12174, 1211, 8315, 16472, 3224, 16712, 5172, 995, 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macromolecules ما بيقدروا يدخلوا جوا الخلية عشان هيك لازم يطلع الانزيم لبرا ويصير التفاعل extracellular من الأمثل على البوليساكرايد ال starch من الأمثل على الليبد ال triglyceride من الأمثل على ال protein fibrinogen casein gelatin الamylase انزيم بيشتغل على ال starch", "tokens": [3224, 3215, 12610, 7251, 34268, 26890, 14407, 7912, 4397, 4812, 11570, 19446, 4724, 1829, 28543, 2288, 14407, 7251, 3215, 9778, 1211, 14407, 10874, 14407, 33962, 1211, 10632, 6225, 8592, 7649, 39896, 4117, 5296, 31377, 2304, 7251, 9566, 1211, 3615, 2423, 7649, 11622, 32640, 5296, 3555, 23557, 4032, 1829, 9381, 13546, 16712, 5172, 995, 30241, 2857, 4164, 1040, 9154, 16247, 2304, 12984, 1211, 15844, 29739, 12610, 1829, 3794, 995, 4117, 23557, 25708, 2423, 24748, 9154, 16247, 2304, 12984, 1211, 15844, 13672, 1829, 44510, 2423, 1376, 70, 356, 1776, 482, 9154, 16247, 2304, 12984, 1211, 15844, 2423, 7944, 283, 6414, 259, 8799, 1389, 259, 45174, 2423, 7804, 875, 405, 16472, 11622, 32640, 4724, 1829, 8592, 2655, 17082, 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420, 8280, 327, 420, 7944, 7251, 3555, 4587, 7578, 8032, 47302, 6055, 34268, 26890, 1090, 19046, 3364, 7251, 3555, 4587, 7578, 2423, 24521, 7251, 34268, 26890, 2111, 78, 268, 1229, 1398], "avg_logprob": -0.13473135585847654, "compression_ratio": 1.6623931623931625, "no_speech_prob": 1.1920928955078125e-07, "words": [{"start": 121.13, "end": 121.47, "word": "في", "probability": 0.9462890625}, {"start": 121.47, "end": 121.79, "word": " الـ", "probability": 0.84033203125}, {"start": 121.79, "end": 122.65, "word": " Exoenzyme", "probability": 0.86611328125}, {"start": 122.65, "end": 122.95, "word": " كان", "probability": 0.60205078125}, {"start": 122.95, "end": 123.41, "word": " ذاكر", "probability": 0.9718424479166666}, {"start": 123.41, "end": 124.03, "word": " أمثلة", "probability": 0.9427490234375}, {"start": 124.03, "end": 125.11, "word": " هنلاقي", "probability": 0.9090576171875}, {"start": 125.11, "end": 125.53, "word": " هذه", "probability": 0.408203125}, {"start": 125.53, "end": 126.57, "word": " الانزيمات", "probability": 0.7984375}, {"start": 126.57, "end": 127.13, "word": " هي", "probability": 0.85205078125}, {"start": 127.13, "end": 128.37, "word": " مذكورة", "probability": 0.96904296875}, {"start": 128.37, "end": 128.63, "word": " في", "probability": 0.98291015625}, {"start": 128.63, "end": 129.33, "word": " الجملة", "probability": 0.92431640625}, {"start": 129.33, "end": 130.05, "word": " يبقى", "probability": 0.946533203125}, {"start": 130.05, "end": 130.39, "word": " عشان", "probability": 0.9762369791666666}, {"start": 130.39, "end": 131.09, "word": " نحفظ", "probability": 0.994873046875}, {"start": 131.09, "end": 131.39, "word": " الـ", "probability": 0.826904296875}, {"start": 131.39, "end": 132.43, "word": " Exoenzyme", "probability": 0.95458984375}, {"start": 132.43, "end": 133.35, "word": " بشوف", "probability": 0.97900390625}, {"start": 133.35, "end": 133.55, "word": " شو", "probability": 0.96630859375}, {"start": 133.55, "end": 133.71, "word": " ال", "probability": 0.429931640625}, {"start": 133.71, "end": 134.23, "word": " substrate", "probability": 0.345703125}, {"start": 134.23, "end": 134.61, "word": " اللي", "probability": 0.8779296875}, {"start": 134.61, "end": 135.23, "word": " بيشتغل", "probability": 0.9854329427083334}, {"start": 135.23, "end": 135.85, "word": " عليها", "probability": 0.919677734375}, {"start": 135.85, "end": 136.13, "word": " ال", "probability": 0.9775390625}, {"start": 136.13, "end": 136.59, "word": " enzyme", "probability": 0.57373046875}, {"start": 136.59, "end": 137.39, "word": " إذا", "probability": 0.92236328125}, {"start": 137.39, "end": 137.59, "word": " ال", "probability": 0.9775390625}, {"start": 137.59, "end": 138.15, "word": " substrate", "probability": 0.91259765625}, {"start": 138.15, "end": 139.81, "word": " polysaccharide", "probability": 0.92646484375}, {"start": 139.81, "end": 140.19, "word": " or", "probability": 0.76513671875}, {"start": 140.19, "end": 140.87, "word": " lipid", "probability": 0.765869140625}, {"start": 140.87, "end": 141.11, "word": " or", "probability": 0.8720703125}, {"start": 141.11, "end": 141.77, "word": " protein", "probability": 0.411865234375}, {"start": 141.77, "end": 142.51, "word": " يبقى", "probability": 0.9853515625}, {"start": 142.51, "end": 142.71, "word": " هاي", "probability": 0.50537109375}, {"start": 142.71, "end": 143.19, "word": " تعتبر", "probability": 0.927734375}, {"start": 143.19, "end": 143.53, "word": " high", "probability": 0.85986328125}, {"start": 143.53, "end": 144.09, "word": " molecular", "probability": 0.81640625}, {"start": 144.09, "end": 144.61, "word": " weight", "probability": 0.93505859375}, {"start": 144.61, "end": 145.21, "word": " يبقى", "probability": 0.9864501953125}, {"start": 145.21, "end": 145.35, "word": " ال", "probability": 0.9658203125}, {"start": 145.35, "end": 145.75, "word": " enzyme", "probability": 0.91357421875}, {"start": 145.75, "end": 146.31, "word": " يعتبر", "probability": 0.95751953125}, {"start": 146.31, "end": 147.95, "word": " Exoenzyme", "probability": 0.8564453125}], "temperature": 1.0}, {"id": 7, "seek": 16204, "start": 148.28, "end": 162.04, "text": "اليوم ان شاء الله في هذه المحاضرة هنتكلم عن الاميلاز and جيلاتيناز هنبدأ بالاميلاز production or starch hydrolysis test أول معلومة عرفناها عن الاميلاز", "tokens": [6027, 1829, 20498, 16472, 13412, 16606, 21984, 8978, 29538, 9673, 5016, 46958, 25720, 8032, 29399, 4117, 19528, 18871, 2423, 10943, 26895, 31377, 293, 10874, 26895, 9307, 9957, 31377, 8032, 1863, 44510, 10721, 20666, 10943, 26895, 31377, 4265, 420, 24748, 15435, 356, 17122, 1500, 5551, 12610, 20449, 1211, 20498, 3660, 6225, 28480, 8315, 11296, 18871, 2423, 10943, 26895, 31377], "avg_logprob": -0.13261387055203067, "compression_ratio": 1.4666666666666666, "no_speech_prob": 1.1920928955078125e-07, "words": [{"start": 148.28, "end": 148.72, "word": "اليوم", "probability": 0.96435546875}, {"start": 148.72, "end": 148.82, "word": " ان", "probability": 0.6318359375}, {"start": 148.82, "end": 149.02, "word": " شاء", "probability": 0.98583984375}, {"start": 149.02, "end": 149.18, "word": " الله", "probability": 0.96044921875}, {"start": 149.18, "end": 149.36, "word": " في", "probability": 0.900390625}, {"start": 149.36, "end": 149.5, "word": " هذه", "probability": 0.83837890625}, {"start": 149.5, "end": 150.16, "word": " المحاضرة", "probability": 0.9793701171875}, {"start": 150.16, "end": 150.82, "word": " هنتكلم", "probability": 0.9725341796875}, {"start": 150.82, "end": 151.12, "word": " عن", "probability": 0.9912109375}, {"start": 151.12, "end": 152.02, "word": " الاميلاز", "probability": 0.73388671875}, {"start": 152.02, "end": 152.52, "word": " and", "probability": 0.41845703125}, {"start": 152.52, "end": 153.28, "word": " جيلاتيناز", "probability": 0.7765625}, {"start": 153.28, "end": 154.52, "word": " هنبدأ", "probability": 0.9346923828125}, {"start": 154.52, "end": 155.58, "word": " بالاميلاز", "probability": 0.8499755859375}, {"start": 155.58, "end": 156.24, "word": " production", "probability": 0.9306640625}, {"start": 156.24, "end": 156.88, "word": " or", "probability": 0.57177734375}, {"start": 156.88, "end": 157.56, "word": " starch", "probability": 0.86083984375}, {"start": 157.56, "end": 158.44, "word": " hydrolysis", "probability": 0.8955078125}, {"start": 158.44, "end": 158.94, "word": " test", "probability": 0.943359375}, {"start": 158.94, "end": 159.78, "word": " أول", "probability": 0.797607421875}, {"start": 159.78, "end": 160.34, "word": " معلومة", "probability": 0.9842529296875}, {"start": 160.34, "end": 160.92, "word": " عرفناها", "probability": 0.973388671875}, {"start": 160.92, "end": 161.14, "word": " عن", "probability": 0.98974609375}, {"start": 161.14, "end": 162.04, "word": " الاميلاز", "probability": 0.943359375}], "temperature": 1.0}, {"id": 8, "seek": 18878, "start": 162.4, "end": 188.78, "text": "إنه Exoenzyme السبسترات اللي بيشتغل عليها ال amylase هي ال starch ال starch يعتبر polysaccharide ال amylase بيعمل hydrolysis ل ال starch عشان هي اسم الفحص starch hydrolysis test or amylase production لأنه بيعمل hydrolysis لل starch ال starch عبارة عن its chain of monosaccharide", "tokens": [28814, 1863, 3224, 2111, 78, 268, 1229, 1398, 21136, 3555, 14851, 2288, 9307, 13672, 1829, 4724, 1829, 8592, 2655, 17082, 1211, 25894, 11296, 2423, 669, 5088, 651, 39896, 2423, 24748, 2423, 24748, 7251, 34268, 26890, 6754, 82, 326, 7374, 482, 2423, 669, 5088, 651, 4724, 1829, 25957, 1211, 15435, 356, 17122, 5296, 2423, 24748, 6225, 8592, 7649, 39896, 24525, 2304, 27188, 5016, 9381, 24748, 15435, 356, 17122, 1500, 420, 669, 5088, 651, 4265, 5296, 33456, 3224, 4724, 1829, 25957, 1211, 15435, 356, 17122, 24976, 24748, 2423, 24748, 6225, 3555, 9640, 3660, 18871, 1080, 5021, 295, 1108, 329, 326, 7374, 482], "avg_logprob": -0.18347771746097224, "compression_ratio": 1.7658536585365854, "no_speech_prob": 8.344650268554688e-07, "words": [{"start": 162.4, "end": 163.18, 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starch", "probability": 0.87744140625}, {"start": 169.68, "end": 170.26, "word": " يعتبر", "probability": 0.9541015625}, {"start": 170.26, "end": 171.3, "word": " polysaccharide", "probability": 0.9546875}, {"start": 171.3, "end": 172.26, "word": " ال", "probability": 0.71142578125}, {"start": 172.26, "end": 172.9, "word": " amylase", "probability": 0.92578125}, {"start": 172.9, "end": 173.52, "word": " بيعمل", "probability": 0.97119140625}, {"start": 173.52, "end": 174.48, "word": " hydrolysis", "probability": 0.7316080729166666}, {"start": 174.48, "end": 174.94, "word": " ل", "probability": 0.8505859375}, {"start": 174.94, "end": 175.52, "word": " ال", "probability": 0.418701171875}, {"start": 175.52, "end": 175.94, "word": " starch", "probability": 0.96875}, {"start": 175.94, "end": 176.38, "word": " عشان", "probability": 0.9454752604166666}, {"start": 176.38, "end": 176.66, "word": " هي", "probability": 0.494873046875}, {"start": 176.66, "end": 177.14, "word": " اسم", 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disaccharide بتتكون من two monosaccharide مرتبطين من الأمثلة على الـ disaccharide", "tokens": [27842, 2423, 39184, 1108, 329, 326, 7374, 482, 25724, 20292, 4117, 2407, 11622, 45164, 47525, 8315, 8717, 2655, 3615, 28480, 15844, 2423, 39184, 2010, 295, 5076, 2423, 39184, 3467, 295, 5076, 8978, 3224, 43242, 7578, 1108, 329, 326, 7374, 482, 11, 717, 326, 7374, 482, 4032, 6754, 82, 326, 7374, 482, 2423, 39184, 1108, 329, 326, 7374, 482, 10874, 20292, 4117, 2407, 11622, 7660, 578, 541, 1975, 5172, 23557, 4117, 2655, 2407, 11622, 39896, 5551, 2304, 12984, 37977, 15844, 1108, 329, 326, 7374, 482, 2423, 39184, 717, 326, 7374, 482, 39894, 2655, 30544, 9154, 732, 1108, 329, 326, 7374, 482, 3714, 43500, 3555, 9566, 9957, 9154, 16247, 2304, 12984, 37977, 15844, 2423, 39184, 717, 326, 7374, 482], "avg_logprob": -0.20709745636430837, "compression_ratio": 2.0202020202020203, "no_speech_prob": 0.0, "words": [{"start": 189.25, "end": 189.55, "word": "من", "probability": 0.71826171875}, {"start": 189.55, 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types", "probability": 0.830078125}, {"start": 196.45, "end": 196.73, "word": " of", "probability": 0.98046875}, {"start": 196.73, "end": 197.17, "word": " sugar", "probability": 0.79296875}, {"start": 197.17, "end": 197.43, "word": " فيه", "probability": 0.551025390625}, {"start": 197.43, "end": 197.71, "word": " عندى", "probability": 0.58349609375}, {"start": 197.71, "end": 198.89, "word": " monosaccharide,", "probability": 0.9146484375}, {"start": 199.35, "end": 200.27, "word": " disaccharide", "probability": 0.9638671875}, {"start": 200.27, "end": 200.41, "word": " و", "probability": 0.74169921875}, {"start": 200.41, "end": 201.35, "word": " polysaccharide", "probability": 0.94775390625}, {"start": 201.35, "end": 201.99, "word": " الـ", "probability": 0.587158203125}, {"start": 201.99, "end": 202.89, "word": " monosaccharide", "probability": 0.97333984375}, {"start": 202.89, "end": 204.33, "word": " جليكوز", "probability": 0.8775390625}, {"start": 204.33, "end": 205.37, "word": " galactose", "probability": 0.6562296549479166}, {"start": 205.37, "end": 206.23, "word": " افراكتوز", "probability": 0.7916085379464286}, {"start": 206.23, "end": 206.63, "word": " هي", "probability": 0.57763671875}, {"start": 206.63, "end": 207.17, "word": " أمثلة", "probability": 0.9296875}, {"start": 207.17, "end": 207.43, "word": " على", "probability": 0.9169921875}, {"start": 207.43, "end": 208.53, "word": " monosaccharide", "probability": 0.8951171875}, {"start": 208.53, "end": 209.05, "word": " الـ", "probability": 0.708984375}, {"start": 209.05, "end": 209.75, "word": " disaccharide", "probability": 0.9647216796875}, {"start": 209.75, "end": 210.39, "word": " بتتكون", "probability": 0.9044596354166666}, {"start": 210.39, "end": 210.75, "word": " من", "probability": 0.9921875}, {"start": 210.75, "end": 211.09, "word": " two", "probability": 0.78173828125}, {"start": 211.09, "end": 212.29, "word": " monosaccharide", "probability": 0.909765625}, {"start": 212.29, "end": 213.15, "word": " مرتبطين", "probability": 0.9873046875}, {"start": 213.15, "end": 214.43, "word": " من", "probability": 0.9873046875}, {"start": 214.43, "end": 214.99, "word": " الأمثلة", "probability": 0.99072265625}, {"start": 214.99, "end": 215.27, "word": " على", "probability": 0.93212890625}, {"start": 215.27, "end": 215.55, "word": " الـ", "probability": 0.56951904296875}, {"start": 215.55, "end": 216.37, "word": " disaccharide", "probability": 0.9644775390625}], "temperature": 1.0}, {"id": 10, "seek": 24662, "start": 217.04, "end": 246.62, "text": "Saccharose, Maltose, Lactose الـ Saccharose عبارة عن جليكوز and فركتوز المالتوز عبارة عن جليكوز زائد جليكوز اللاكتوز جليكوز زائد جلاكتوز تالت نوع الـ Polysaccharide مثال عليها الـ starch قولنا أن الـ starch عبارة عن polymer of مونو ساكرايد من المونو ساكرايد الجليكوز من الأمثلة الأخرى على ال polysaccharide في عندي الجليكوجين وعندي السليلوز", "tokens": [50, 326, 7374, 541, 11, 376, 3198, 541, 11, 441, 578, 541, 2423, 39184, 318, 326, 7374, 541, 6225, 3555, 9640, 3660, 18871, 10874, 20292, 4117, 2407, 11622, 293, 6156, 2288, 4117, 2655, 2407, 11622, 9673, 6027, 2655, 2407, 11622, 6225, 3555, 9640, 3660, 18871, 10874, 20292, 4117, 2407, 11622, 30767, 16373, 3215, 10874, 20292, 4117, 2407, 11622, 13672, 995, 4117, 2655, 2407, 11622, 10874, 20292, 4117, 2407, 11622, 30767, 16373, 3215, 10874, 15040, 4117, 2655, 2407, 11622, 6055, 6027, 2655, 8717, 45367, 2423, 39184, 18553, 82, 326, 7374, 482, 50113, 6027, 25894, 11296, 2423, 39184, 24748, 12174, 12610, 8315, 14739, 2423, 39184, 24748, 6225, 3555, 9640, 3660, 18871, 6754, 936, 295, 3714, 11536, 2407, 8608, 995, 37983, 995, 25708, 9154, 9673, 11536, 2407, 8608, 995, 37983, 995, 25708, 25724, 20292, 4117, 2407, 11622, 9154, 16247, 2304, 12984, 37977, 16247, 34740, 7578, 15844, 2423, 6754, 82, 326, 7374, 482, 8978, 43242, 1829, 25724, 20292, 4117, 29245, 9957, 4032, 3615, 1863, 16254, 21136, 20292, 1211, 2407, 11622], "avg_logprob": -0.25149701883692943, "compression_ratio": 2.1774193548387095, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 217.04, "end": 217.9, "word": "Saccharose,", "probability": 0.7650146484375}, {"start": 218.02, "end": 218.56, "word": " Maltose,", "probability": 0.7236328125}, {"start": 218.66, "end": 219.16, "word": " Lactose", "probability": 0.9383138020833334}, {"start": 219.16, "end": 219.44, "word": " الـ", "probability": 0.259979248046875}, {"start": 219.44, "end": 220.14, "word": " Saccharose", "probability": 0.7664794921875}, {"start": 220.14, "end": 220.76, "word": " عبارة", "probability": 0.9947509765625}, {"start": 220.76, "end": 221.0, "word": " عن", "probability": 0.994140625}, {"start": 221.0, "end": 221.68, "word": " جليكوز", "probability": 0.85283203125}, {"start": 221.68, "end": 222.02, "word": " and", "probability": 0.3330078125}, {"start": 222.02, "end": 222.74, "word": " فركتوز", "probability": 0.6883748372395834}, {"start": 222.74, "end": 223.46, "word": " المالتوز", "probability": 0.81533203125}, {"start": 223.46, "end": 223.98, "word": " عبارة", "probability": 0.995849609375}, {"start": 223.98, "end": 224.24, "word": " عن", "probability": 0.9912109375}, {"start": 224.24, "end": 225.04, "word": " جليكوز", "probability": 0.98564453125}, {"start": 225.04, "end": 225.46, "word": " زائد", "probability": 0.6300862630208334}, {"start": 225.46, "end": 226.32, "word": " جليكوز", "probability": 0.97998046875}, {"start": 226.32, "end": 227.48, "word": " اللاكتوز", "probability": 0.83203125}, {"start": 227.48, "end": 228.14, "word": " جليكوز", "probability": 0.97119140625}, {"start": 228.14, "end": 228.66, "word": " زائد", "probability": 0.9119466145833334}, {"start": 228.66, "end": 229.5, "word": " جلاكتوز", "probability": 0.8755696614583334}, {"start": 229.5, "end": 230.68, "word": " تالت", "probability": 0.9609375}, {"start": 230.68, "end": 230.96, "word": " نوع", "probability": 0.98828125}, {"start": 230.96, "end": 231.2, "word": " الـ", "probability": 0.525390625}, {"start": 231.2, "end": 232.12, "word": " Polysaccharide", "probability": 0.85908203125}, {"start": 232.12, "end": 232.82, "word": " مثال", "probability": 0.9189453125}, {"start": 232.82, "end": 233.18, "word": " عليها", "probability": 0.734375}, {"start": 233.18, "end": 233.4, "word": " الـ", "probability": 0.55816650390625}, {"start": 233.4, "end": 233.82, "word": " starch", "probability": 0.7880859375}, {"start": 233.82, "end": 234.26, "word": " قولنا", "probability": 0.86767578125}, {"start": 234.26, "end": 234.44, "word": " أن", "probability": 0.3251953125}, {"start": 234.44, "end": 234.72, "word": " الـ", "probability": 0.696044921875}, {"start": 234.72, "end": 235.06, "word": " starch", "probability": 0.89794921875}, {"start": 235.06, "end": 235.68, "word": " عبارة", "probability": 0.99755859375}, {"start": 235.68, "end": 236.1, "word": " عن", "probability": 0.98583984375}, {"start": 236.1, "end": 237.32, "word": " polymer", "probability": 0.62060546875}, {"start": 237.32, "end": 237.74, "word": " of", "probability": 0.82177734375}, {"start": 237.74, "end": 238.24, "word": " مونو", "probability": 0.5069986979166666}, {"start": 238.24, "end": 238.88, "word": " ساكرايد", "probability": 0.7103515625}, {"start": 238.88, "end": 239.1, "word": " من", "probability": 0.92236328125}, {"start": 239.1, "end": 239.56, "word": " المونو", "probability": 0.801025390625}, {"start": 239.56, "end": 240.14, "word": " ساكرايد", "probability": 0.96650390625}, {"start": 240.14, "end": 240.9, "word": " الجليكوز", "probability": 0.9470703125}, {"start": 240.9, "end": 241.52, "word": " من", "probability": 0.97802734375}, {"start": 241.52, "end": 242.0, "word": " الأمثلة", "probability": 0.956298828125}, {"start": 242.0, "end": 242.58, "word": " الأخرى", "probability": 0.9895833333333334}, {"start": 242.58, "end": 242.86, "word": " على", "probability": 0.90478515625}, {"start": 242.86, "end": 243.08, "word": " ال", "probability": 0.775390625}, {"start": 243.08, "end": 243.94, "word": " polysaccharide", "probability": 0.856884765625}, {"start": 243.94, "end": 244.12, "word": " في", "probability": 0.890625}, {"start": 244.12, "end": 244.42, "word": " عندي", "probability": 0.543212890625}, {"start": 244.42, "end": 245.2, "word": " الجليكوجين", "probability": 0.7847412109375}, {"start": 245.2, "end": 245.68, "word": " وعندي", "probability": 0.6004638671875}, {"start": 245.68, "end": 246.62, "word": " السليلوز", "probability": 0.790625}], "temperature": 1.0}, {"id": 11, "seek": 27433, "start": 247.2, "end": 274.34, "text": "لكن اليوم ان شاء الله هنتكلم احنا عن ال amylase اللى بيشتغل على ال starch ال starch يعتبر polysaccharide بتكون من polymer of glycose ال starch هيدروليزز تكسير ال starch بيصير بواسط ال two type of enzyme بواسط اتنوعين من الانزيمات اول انزيم Alpha amylase Alpha وليس beta لأن ال beta", "tokens": [1211, 19452, 45595, 20498, 16472, 13412, 16606, 21984, 8032, 29399, 4117, 19528, 1975, 5016, 8315, 18871, 2423, 669, 5088, 651, 13672, 7578, 4724, 1829, 8592, 2655, 17082, 1211, 15844, 2423, 24748, 2423, 24748, 7251, 34268, 26890, 6754, 82, 326, 7374, 482, 39894, 30544, 9154, 6754, 936, 295, 290, 356, 66, 541, 2423, 24748, 8032, 25708, 2288, 12610, 1829, 11622, 11622, 6055, 4117, 3794, 13546, 2423, 24748, 4724, 1829, 9381, 13546, 4724, 14407, 3794, 9566, 2423, 732, 2010, 295, 24521, 4724, 14407, 3794, 9566, 1975, 2655, 1863, 45367, 9957, 9154, 2423, 7649, 11622, 32640, 9307, 1975, 12610, 16472, 11622, 32640, 20588, 669, 5088, 651, 20588, 4032, 20292, 3794, 9861, 5296, 33456, 2423, 9861], "avg_logprob": -0.19745575221238937, "compression_ratio": 1.7341772151898733, "no_speech_prob": 1.7881393432617188e-07, "words": [{"start": 247.2, "end": 247.72, "word": "لكن", "probability": 0.7308349609375}, {"start": 247.72, "end": 248.04, "word": " اليوم", "probability": 0.937744140625}, {"start": 248.04, "end": 248.18, "word": " ان", "probability": 0.568359375}, {"start": 248.18, "end": 248.38, "word": " شاء", "probability": 0.927978515625}, {"start": 248.38, "end": 248.6, "word": " الله", "probability": 0.9443359375}, {"start": 248.6, "end": 249.56, "word": " هنتكلم", "probability": 0.9671630859375}, {"start": 249.56, "end": 250.0, "word": " احنا", "probability": 0.9420572916666666}, {"start": 250.0, "end": 250.34, "word": " عن", "probability": 0.990234375}, {"start": 250.34, "end": 250.6, "word": " ال", "probability": 0.96630859375}, {"start": 250.6, "end": 251.36, "word": " amylase", "probability": 0.72216796875}, {"start": 251.36, "end": 252.28, "word": " اللى", "probability": 0.808349609375}, {"start": 252.28, "end": 253.18, "word": " بيشتغل", "probability": 0.98974609375}, {"start": 253.18, "end": 253.6, "word": " على", "probability": 0.83203125}, {"start": 253.6, "end": 253.82, "word": " ال", "probability": 0.9169921875}, {"start": 253.82, "end": 254.36, "word": " starch", "probability": 0.83544921875}, {"start": 254.36, "end": 254.54, "word": " ال", "probability": 0.7158203125}, {"start": 254.54, "end": 254.9, "word": " starch", "probability": 0.89404296875}, {"start": 254.9, "end": 255.42, "word": " يعتبر", "probability": 0.8956705729166666}, {"start": 255.42, "end": 256.32, "word": " polysaccharide", "probability": 0.9626953125}, {"start": 256.32, "end": 256.88, "word": " بتكون", "probability": 0.87841796875}, {"start": 256.88, "end": 257.28, "word": " من", "probability": 0.9912109375}, {"start": 257.28, "end": 258.24, "word": " polymer", "probability": 0.794921875}, {"start": 258.24, "end": 258.58, "word": " of", "probability": 0.9404296875}, {"start": 258.58, "end": 259.7, "word": " glycose", "probability": 0.59039306640625}, {"start": 259.7, "end": 260.14, "word": " ال", "probability": 0.890625}, {"start": 260.14, "end": 260.58, "word": " starch", "probability": 0.94482421875}, {"start": 260.58, "end": 261.84, "word": " هيدروليزز", "probability": 0.6625453404017857}, {"start": 261.84, "end": 262.42, "word": " تكسير", "probability": 0.971435546875}, {"start": 262.42, "end": 262.6, "word": " ال", "probability": 0.96435546875}, {"start": 262.6, "end": 263.06, "word": " starch", "probability": 0.966796875}, {"start": 263.06, "end": 263.62, "word": " بيصير", "probability": 0.8309326171875}, {"start": 263.62, "end": 264.2, "word": " بواسط", "probability": 0.9852294921875}, {"start": 264.2, "end": 264.38, "word": " ال", "probability": 0.68310546875}, {"start": 264.38, "end": 264.66, "word": " two", "probability": 0.8701171875}, {"start": 264.66, "end": 265.14, "word": " type", "probability": 0.6484375}, {"start": 265.14, "end": 265.4, "word": " of", "probability": 0.89599609375}, {"start": 265.4, "end": 265.9, "word": " enzyme", "probability": 0.81982421875}, {"start": 265.9, "end": 266.48, "word": " بواسط", "probability": 0.9869384765625}, {"start": 266.48, "end": 267.12, "word": " اتنوعين", "probability": 0.9197265625}, {"start": 267.12, "end": 267.44, "word": " من", "probability": 0.99462890625}, {"start": 267.44, "end": 268.38, "word": " الانزيمات", "probability": 0.865625}, {"start": 268.38, "end": 269.4, "word": " اول", "probability": 0.83154296875}, {"start": 269.4, "end": 269.98, "word": " انزيم", "probability": 0.939453125}, {"start": 269.98, "end": 270.66, "word": " Alpha", "probability": 0.5517578125}, {"start": 270.66, "end": 271.44, "word": " amylase", "probability": 0.7706705729166666}, {"start": 271.44, "end": 271.98, "word": " Alpha", "probability": 0.72216796875}, {"start": 271.98, "end": 272.56, "word": " وليس", "probability": 0.9026692708333334}, {"start": 272.56, "end": 273.06, "word": " beta", "probability": 0.252197265625}, {"start": 273.06, "end": 273.76, "word": " لأن", "probability": 0.76123046875}, {"start": 273.76, "end": 273.94, "word": " ال", "probability": 0.81884765625}, {"start": 273.94, "end": 274.34, "word": " beta", "probability": 0.8681640625}], "temperature": 1.0}, {"id": 12, "seek": 30327, "start": 274.72, "end": 303.28, "text": "يله علاقة بالـ cellulose الـ cellulose الموجود في البلاند الـ alpha amylase بيعمل hydrolysis of α1,4-glycosidic bond بيكسر الرابطة اللي اسمها α1,4-glycosidic bond الانزيم التاني المسؤول عن تكسير ال starch Oligo-1,6-glycosidase مسؤول عن تكسير الرابطة α1,6-glycosidic bond", "tokens": [26895, 3224, 11203, 995, 28671, 20666, 39184, 2815, 425, 541, 2423, 39184, 2815, 425, 541, 9673, 29245, 23328, 8978, 29739, 1211, 7649, 3215, 2423, 39184, 8961, 669, 5088, 651, 4724, 1829, 25957, 1211, 15435, 356, 17122, 295, 5691, 16, 11, 19, 12, 70, 356, 6877, 327, 299, 6086, 4724, 1829, 4117, 3794, 2288, 34892, 16758, 9566, 3660, 13672, 1829, 24525, 2304, 11296, 5691, 16, 11, 19, 12, 70, 356, 6877, 327, 299, 6086, 2423, 7649, 11622, 32640, 16712, 7649, 1829, 9673, 3794, 33604, 12610, 18871, 6055, 4117, 3794, 13546, 2423, 24748, 422, 5073, 78, 12, 16, 11, 21, 12, 70, 356, 6877, 327, 651, 47524, 33604, 12610, 18871, 6055, 4117, 3794, 13546, 34892, 16758, 9566, 3660, 5691, 16, 11, 21, 12, 70, 356, 6877, 327, 299, 6086], "avg_logprob": -0.21228026831522584, "compression_ratio": 1.7477064220183487, "no_speech_prob": 2.980232238769531e-07, "words": [{"start": 274.72, "end": 275.06, "word": "يله", "probability": 0.3909912109375}, {"start": 275.06, "end": 275.62, "word": " علاقة", "probability": 0.98583984375}, {"start": 275.62, "end": 276.26, "word": " بالـ", "probability": 0.66015625}, {"start": 276.26, "end": 276.78, "word": " cellulose", "probability": 0.85595703125}, {"start": 276.78, "end": 277.24, "word": " الـ", "probability": 0.57275390625}, {"start": 277.24, "end": 277.68, "word": " cellulose", "probability": 0.9632161458333334}, {"start": 277.68, "end": 278.18, "word": " الموجود", "probability": 0.8951822916666666}, {"start": 278.18, "end": 278.42, "word": " في", "probability": 0.91357421875}, {"start": 278.42, "end": 279.14, "word": " البلاند", "probability": 0.678558349609375}, {"start": 279.14, "end": 279.88, "word": " الـ", "probability": 0.87255859375}, {"start": 279.88, "end": 280.1, "word": " alpha", "probability": 0.345703125}, {"start": 280.1, "end": 280.82, "word": " amylase", "probability": 0.8401692708333334}, {"start": 280.82, "end": 282.4, "word": " بيعمل", "probability": 0.931396484375}, {"start": 282.4, "end": 283.34, "word": " hydrolysis", "probability": 0.6490885416666666}, {"start": 283.34, "end": 283.72, "word": " of", "probability": 0.92333984375}, {"start": 283.72, "end": 284.6, "word": " α1", "probability": 0.45452880859375}, {"start": 284.6, "end": 284.98, "word": ",4", "probability": 0.5714111328125}, {"start": 284.98, "end": 285.88, "word": "-glycosidic", "probability": 0.921142578125}, {"start": 285.88, "end": 286.08, "word": " bond", "probability": 0.91455078125}, {"start": 286.08, "end": 286.9, "word": " بيكسر", "probability": 0.8708984375}, {"start": 286.9, "end": 288.1, "word": " الرابطة", "probability": 0.9622802734375}, {"start": 288.1, "end": 288.36, "word": " اللي", "probability": 0.701416015625}, {"start": 288.36, "end": 288.82, "word": " اسمها", "probability": 0.9583333333333334}, {"start": 288.82, "end": 289.5, "word": " α1", "probability": 0.774658203125}, {"start": 289.5, "end": 289.88, "word": ",4", "probability": 0.994140625}, {"start": 289.88, "end": 290.74, "word": "-glycosidic", "probability": 0.9735514322916666}, {"start": 290.74, "end": 291.38, "word": " bond", "probability": 0.91796875}, {"start": 291.38, "end": 292.7, "word": " الانزيم", "probability": 0.7918701171875}, {"start": 292.7, "end": 293.16, "word": " التاني", "probability": 0.9580078125}, {"start": 293.16, "end": 293.8, "word": " المسؤول", "probability": 0.8680419921875}, {"start": 293.8, "end": 294.02, "word": " عن", "probability": 0.99609375}, {"start": 294.02, "end": 294.52, "word": " تكسير", "probability": 0.9918212890625}, {"start": 294.52, "end": 294.64, "word": " ال", "probability": 0.9248046875}, {"start": 294.64, "end": 295.2, "word": " starch", "probability": 0.453125}, {"start": 295.2, "end": 295.96, "word": " Oligo", "probability": 0.6191813151041666}, {"start": 295.96, "end": 296.22, "word": "-1", "probability": 0.5035400390625}, {"start": 296.22, "end": 296.68, "word": ",6", "probability": 0.986328125}, {"start": 296.68, "end": 297.98, "word": "-glycosidase", "probability": 0.9547526041666666}, {"start": 297.98, "end": 299.16, "word": " مسؤول", "probability": 0.87158203125}, {"start": 299.16, "end": 299.38, "word": " عن", "probability": 0.99169921875}, {"start": 299.38, "end": 299.88, "word": " تكسير", "probability": 0.99853515625}, {"start": 299.88, "end": 300.72, "word": " الرابطة", "probability": 0.9241943359375}, {"start": 300.72, "end": 301.48, "word": " α1", "probability": 0.75830078125}, {"start": 301.48, "end": 301.92, "word": ",6", "probability": 0.994384765625}, {"start": 301.92, "end": 303.06, "word": "-glycosidic", "probability": 0.9829915364583334}, {"start": 303.06, "end": 303.28, "word": " bond", "probability": 0.93212890625}], "temperature": 1.0}, {"id": 13, "seek": 32644, "start": 303.82, "end": 326.44, "text": "عشان نوضح الصورة اكتر هذه الصورة بتوضح الـ starch structure الـ starch قولنا عبارة عن its chain of monosaccharide الـ monosaccharide اللي هو الجليكوز يبقى هو عبارة عن سلسلة من الجليكوز هاي الوحدات الخضراء عبارة عن الجليكوز", "tokens": [3615, 8592, 7649, 8717, 2407, 11242, 5016, 31767, 13063, 3660, 1975, 4117, 2655, 2288, 29538, 31767, 13063, 3660, 39894, 2407, 11242, 5016, 2423, 39184, 24748, 3877, 2423, 39184, 24748, 12174, 12610, 8315, 6225, 3555, 9640, 3660, 18871, 1080, 5021, 295, 1108, 329, 326, 7374, 482, 2423, 39184, 1108, 329, 326, 7374, 482, 13672, 1829, 31439, 25724, 20292, 4117, 2407, 11622, 7251, 3555, 4587, 7578, 31439, 6225, 3555, 9640, 3660, 18871, 8608, 1211, 3794, 37977, 9154, 25724, 20292, 4117, 2407, 11622, 8032, 47302, 2423, 2407, 24401, 9307, 33962, 11242, 2288, 16606, 6225, 3555, 9640, 3660, 18871, 25724, 20292, 4117, 2407, 11622], "avg_logprob": -0.15091274929518747, "compression_ratio": 1.9175824175824177, "no_speech_prob": 2.980232238769531e-07, "words": [{"start": 303.82, "end": 304.2, "word": "عشان", 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{"id": 14, "seek": 35472, "start": 327.64, "end": 354.72, "text": "إذا كان ال ال structure chain الرابط ما بينهم بتكون alpha one four linkage لو كان في عندي branch بتكون الرابط alpha one six linkage احنا قلنا انه في عندي two type two enzyme مسئولين عن ال starch hydrolysis الانزيم الأول اللي هو ال alpha amylase ال alpha amylase قلنا مسئول عن تكسير ال", "tokens": [28814, 15730, 25961, 2423, 2423, 3877, 5021, 34892, 16758, 9566, 19446, 49374, 16095, 39894, 30544, 8961, 472, 1451, 49118, 45164, 25961, 8978, 18871, 16254, 9819, 39894, 30544, 34892, 16758, 9566, 8961, 472, 2309, 49118, 1975, 5016, 8315, 12174, 1211, 8315, 16472, 3224, 8978, 18871, 16254, 732, 2010, 732, 24521, 47524, 19986, 12610, 9957, 18871, 2423, 24748, 15435, 356, 17122, 2423, 7649, 11622, 32640, 16247, 12610, 13672, 1829, 31439, 2423, 8961, 669, 5088, 651, 2423, 8961, 669, 5088, 651, 12174, 1211, 8315, 47524, 19986, 12610, 18871, 6055, 4117, 3794, 13546, 2423], "avg_logprob": -0.20192307594058279, "compression_ratio": 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0.9899088541666666}, {"start": 491.18, "end": 491.88, "word": " Certain", "probability": 0.25732421875}, {"start": 491.88, "end": 492.92, "word": " Exoenzyme", "probability": 0.85009765625}, {"start": 492.92, "end": 493.4, "word": " قولنا", "probability": 0.7945963541666666}, {"start": 493.4, "end": 493.56, "word": " إن", "probability": 0.55810546875}, {"start": 493.56, "end": 494.04, "word": " الـ", "probability": 0.7060546875}, {"start": 494.04, "end": 494.5, "word": " Amylase", "probability": 0.7960611979166666}, {"start": 494.5, "end": 494.96, "word": " يعتبر", "probability": 0.9075520833333334}, {"start": 494.96, "end": 496.16, "word": " Exoenzyme", "probability": 0.92578125}, {"start": 496.16, "end": 497.22, "word": " الإنزيمات", "probability": 0.89375}, {"start": 497.22, "end": 498.02, "word": " المسؤولة", "probability": 0.939453125}, {"start": 498.02, "end": 498.34, "word": " عن", "probability": 0.98779296875}, {"start": 498.34, "end": 498.74, "word": " الـ", "probability": 0.5030517578125}, {"start": 498.74, "end": 499.22, "word": " starch", "probability": 0.55859375}, {"start": 499.22, "end": 500.14, "word": " Hydrolysis", "probability": 0.67596435546875}, {"start": 500.14, "end": 501.04, "word": " أول", "probability": 0.93798828125}, {"start": 501.04, "end": 501.42, "word": " إنزيم", "probability": 0.9244791666666666}, {"start": 501.42, "end": 501.58, "word": " اللي", "probability": 0.7578125}, {"start": 501.58, "end": 501.76, "word": " هو", "probability": 0.990234375}, {"start": 501.76, "end": 501.98, "word": " الـ", "probability": 0.8740234375}, {"start": 501.98, "end": 502.28, "word": " Alpha", "probability": 0.54833984375}, {"start": 502.28, "end": 503.02, "word": " Amylase", "probability": 0.796142578125}, {"start": 503.02, "end": 503.74, "word": " المسؤول", "probability": 0.9156494140625}, {"start": 503.74, "end": 504.0, "word": " عن", "probability": 0.9677734375}, {"start": 504.0, "end": 504.48, "word": " تكسير", "probability": 0.9920654296875}, {"start": 504.48, "end": 505.04, "word": " الرابط", "probability": 0.8141276041666666}, {"start": 505.04, "end": 505.5, "word": " Alpha", "probability": 0.6962890625}, {"start": 505.5, "end": 505.86, "word": "-1", "probability": 0.5814208984375}, {"start": 505.86, "end": 507.12, "word": "-Pharyglycosidic", "probability": 0.7389373779296875}, {"start": 507.12, "end": 507.5, "word": " bond", "probability": 0.448486328125}, {"start": 507.5, "end": 508.56, "word": " الإنزيم", "probability": 0.9365234375}, {"start": 508.56, "end": 509.18, "word": " التاني", "probability": 0.9132486979166666}, {"start": 509.18, "end": 509.36, "word": " يبقى", "probability": 0.8114013671875}, {"start": 509.36, "end": 509.58, "word": " هذا", "probability": 0.912109375}, {"start": 509.58, "end": 509.92, "word": " أول", "probability": 0.927001953125}, {"start": 509.92, "end": 510.3, "word": " إنزيم", "probability": 0.9749348958333334}, {"start": 510.3, "end": 510.44, "word": " اللي", "probability": 0.967041015625}, {"start": 510.44, "end": 510.6, "word": " هو", "probability": 0.994140625}, {"start": 510.6, "end": 510.84, "word": " الـ", "probability": 0.80029296875}, {"start": 510.84, "end": 511.12, "word": " Alpha", "probability": 0.876953125}, {"start": 511.12, "end": 511.84, "word": " Amylase", "probability": 0.80224609375}], "temperature": 1.0}, {"id": 21, "seek": 54459, "start": 515.09, "end": 544.59, "text": "الانزيم التاني اللي هو Oligo-1,6-Glycosidase المسؤول عن تكسير الرابطة Alpha-1,6-Glycosidic bond that hydrolyze starch بيكسروا ال starch لمركبات صغيرة الحجم بتقدر تدخل لخلية starch molecules are too large to enter the bacterial cell قولنا إن ال starch يعتبر polysaccharide حجمه كبير ما بيقدر يدخل الخلية", "tokens": [6027, 7649, 11622, 32640, 16712, 7649, 1829, 13672, 1829, 31439, 422, 5073, 78, 12, 16, 11, 21, 12, 38, 356, 6877, 327, 651, 9673, 3794, 33604, 12610, 18871, 6055, 4117, 3794, 13546, 34892, 16758, 9566, 3660, 20588, 12, 16, 11, 21, 12, 38, 356, 6877, 327, 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{"start": 539.37, "end": 539.49, "word": " ال", "probability": 0.88525390625}, {"start": 539.49, "end": 540.09, "word": " starch", "probability": 0.72119140625}, {"start": 540.09, "end": 540.99, "word": " يعتبر", "probability": 0.9138997395833334}, {"start": 540.99, "end": 541.99, "word": " polysaccharide", "probability": 0.9400390625}, {"start": 541.99, "end": 542.55, "word": " حجمه", "probability": 0.9775390625}, {"start": 542.55, "end": 542.95, "word": " كبير", "probability": 0.9951171875}, {"start": 542.95, "end": 543.13, "word": " ما", "probability": 0.724609375}, {"start": 543.13, "end": 543.67, "word": " بيقدر", "probability": 0.9588623046875}, {"start": 543.67, "end": 544.05, "word": " يدخل", "probability": 0.9964599609375}, {"start": 544.05, "end": 544.59, "word": " الخلية", "probability": 0.9654947916666666}], "temperature": 1.0}, {"id": 22, "seek": 56889, "start": 545.09, "end": 568.89, "text": "So some bacteria some مش كل البكتيريا بتفرز الانزيمات المسؤولة عن تكسير ال starch some bacteria secrete exoenzyme اتفقنا انه عبارة عن exoenzyme to degrade starch into subunit بيكسل ال starch ل subunit زي ما اتعرفنا اما مالتوز مالتو تريوز او ديسترين هاي ال subunit", "tokens": [6455, 512, 11763, 512, 37893, 28242, 29739, 4117, 2655, 13546, 25528, 39894, 5172, 2288, 11622, 2423, 7649, 11622, 32640, 9307, 9673, 3794, 33604, 12610, 3660, 18871, 6055, 4117, 3794, 13546, 2423, 24748, 512, 11763, 907, 7600, 454, 78, 268, 1229, 1398, 1975, 2655, 5172, 4587, 8315, 16472, 3224, 6225, 3555, 9640, 3660, 18871, 454, 78, 268, 1229, 1398, 281, 368, 8692, 24748, 666, 1422, 409, 270, 4724, 1829, 4117, 3794, 1211, 2423, 24748, 5296, 1422, 409, 270, 30767, 1829, 19446, 1975, 2655, 3615, 28480, 8315, 1975, 15042, 3714, 6027, 2655, 2407, 11622, 3714, 6027, 2655, 2407, 6055, 16572, 2407, 11622, 1975, 2407, 11778, 1829, 14851, 2288, 9957, 8032, 47302, 2423, 1422, 409, 270], "avg_logprob": -0.2597313489307437, "compression_ratio": 1.7123287671232876, "no_speech_prob": 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هذا الانزيم وهل البكتيريا قادرة انها تكسر ال starch او لأ", "tokens": [6780, 393, 550, 312, 28158, 538, 264, 24128, 39894, 28543, 2288, 6055, 3215, 9778, 1211, 24976, 9778, 1211, 10632, 16070, 12372, 1434, 7251, 28543, 2288, 16472, 3224, 7251, 14851, 3224, 23275, 11296, 4032, 1829, 14851, 5172, 25708, 9154, 11296, 39896, 4117, 1975, 5016, 8315, 6225, 28480, 8315, 9122, 33911, 2423, 7649, 11622, 32640, 4724, 1829, 4117, 3794, 2288, 2423, 24748, 9122, 33911, 13412, 17082, 1211, 2423, 7649, 11622, 32640, 23032, 3555, 9122, 33911, 47525, 8315, 8717, 4117, 8592, 5172, 18871, 23758, 2423, 7649, 11622, 32640, 4032, 3224, 1211, 29739, 4117, 2655, 13546, 25528, 12174, 18513, 25720, 16472, 11296, 6055, 4117, 3794, 2288, 2423, 24748, 1975, 2407, 5296, 10721], "avg_logprob": -0.13754300404032435, "compression_ratio": 1.7008928571428572, "no_speech_prob": 2.8014183044433594e-06, "words": [{"start": 569.07, "end": 569.33, "word": "that", "probability": 0.331787109375}, {"start": 569.33, "end": 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0.99072265625}, {"start": 761.98, "end": 762.4, "word": " انزيم", "probability": 0.9295247395833334}, {"start": 762.4, "end": 763.34, "word": " الاميلاز", "probability": 0.944091796875}, {"start": 763.34, "end": 764.3, "word": " هتكسر", "probability": 0.988671875}, {"start": 764.3, "end": 764.6, "word": " ال", "probability": 0.98486328125}, {"start": 764.6, "end": 765.12, "word": " starch", "probability": 0.96484375}, {"start": 765.12, "end": 765.44, "word": " يعني", "probability": 0.9599609375}, {"start": 765.44, "end": 765.62, "word": " ال", "probability": 0.93359375}, {"start": 765.62, "end": 766.08, "word": " starch", "probability": 0.93701171875}, {"start": 766.08, "end": 766.42, "word": " مش", "probability": 0.98876953125}, {"start": 766.42, "end": 767.2, "word": " موجود", "probability": 0.984375}, {"start": 767.2, "end": 767.9, "word": " يعني", "probability": 0.97607421875}, {"start": 767.9, "end": 768.1, "word": " مش", "probability": 0.98828125}, {"start": 768.1, "end": 768.54, "word": " هيصير", "probability": 0.9178059895833334}, {"start": 768.54, "end": 769.16, "word": " تفاعل", "probability": 0.9605712890625}, {"start": 769.16, "end": 769.42, "word": " وما", "probability": 0.83203125}, {"start": 769.42, "end": 769.68, "word": " رح", "probability": 0.766845703125}, {"start": 769.68, "end": 770.2, "word": " يعطي", "probability": 0.9505208333333334}, {"start": 770.2, "end": 770.68, "word": " اللون", "probability": 0.986328125}, {"start": 770.68, "end": 771.24, "word": " المعين", "probability": 0.9842122395833334}], "temperature": 1.0}, {"id": 30, "seek": 79793, "start": 771.89, "end": 797.93, "text": "لكن لو البكتيريا ما عندها ال enzyme ال starch ما رح يتكسر هيظل موجود في ال media يعني هيصير التفاعل يعطي لون معين هذا هو ال principle الفحص طيب شو هو ال indicator اللي احنا بنضيفه بقولنا بتضيفي iodine ال iodine أخدناها في ال gram stain نفسه اللي احنا استخدمناه في ال gram stain بقولنا بعد", "tokens": [1211, 19452, 45164, 29739, 4117, 2655, 13546, 25528, 19446, 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{"start": 790.51, "end": 791.07, "word": " ال", "probability": 0.73486328125}, {"start": 791.07, "end": 791.57, "word": " iodine", "probability": 0.9384765625}, {"start": 791.57, "end": 792.19, "word": " أخدناها", "probability": 0.86435546875}, {"start": 792.19, "end": 792.27, "word": " في", "probability": 0.9404296875}, {"start": 792.27, "end": 792.37, "word": " ال", "probability": 0.7880859375}, {"start": 792.37, "end": 792.65, "word": " gram", "probability": 0.5205078125}, {"start": 792.65, "end": 793.09, "word": " stain", "probability": 0.1138916015625}, {"start": 793.09, "end": 793.93, "word": " نفسه", "probability": 0.99267578125}, {"start": 793.93, "end": 794.17, "word": " اللي", "probability": 0.97119140625}, {"start": 794.17, "end": 794.47, "word": " احنا", "probability": 0.9249674479166666}, {"start": 794.47, "end": 795.15, "word": " استخدمناه", "probability": 0.94404296875}, {"start": 795.15, "end": 795.25, "word": " في", "probability": 0.9775390625}, {"start": 795.25, "end": 795.35, "word": " ال", "probability": 0.7529296875}, {"start": 795.35, "end": 795.65, "word": " gram", "probability": 0.89208984375}, {"start": 795.65, "end": 796.21, "word": " stain", "probability": 0.9189453125}, {"start": 796.21, "end": 797.55, "word": " بقولنا", "probability": 0.9602864583333334}, {"start": 797.55, "end": 797.93, "word": " بعد", "probability": 0.86669921875}], "temperature": 1.0}, {"id": 31, "seek": 82116, "start": 798.42, "end": 821.16, "text": "الـ Incubation لمدة 24 ساعة وبعد ما تنمو البكتيريا بغمر الطبق بال Iodine بغمر كل الطبق بال Iodine وبشوف نتيجة التفاعل Iodine will turn blue when it reacts with starch بقوللي ال Iodine بتحول للون الأزرق لو اتفاعل مع ال starch", "tokens": [6027, 39184, 7779, 836, 399, 32767, 41891, 4022, 8608, 995, 27884, 46599, 22488, 19446, 6055, 1863, 2304, 2407, 29739, 4117, 2655, 13546, 25528, 4724, 17082, 29973, 41950, 3555, 4587, 20666, 286, 378, 533, 4724, 17082, 29973, 28242, 41950, 3555, 4587, 20666, 286, 378, 533, 46599, 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كانت النتيجة positive موجود الأميلاز بتكون clear zone ال principle الفحص لسترش لو كانت البكتيريا ما عندها أنزيم الأميلازي بقى كانت negative للأميلاز", "tokens": [32, 1850, 6668, 486, 312, 1612, 689, 24748, 575, 668, 13884, 292, 45164, 2423, 14851, 2288, 8592, 1975, 2655, 4117, 3794, 2288, 4724, 39648, 1829, 28239, 31371, 7435, 3660, 39894, 30544, 1850, 6668, 7251, 3555, 4587, 7578, 13672, 11536, 2423, 3344, 45164, 25961, 2655, 28239, 31371, 7435, 3660, 3671, 44381, 45164, 25961, 2655, 28239, 31371, 7435, 3660, 3353, 3714, 29245, 23328, 16247, 2304, 26895, 31377, 39894, 30544, 1850, 6668, 2423, 8665, 27188, 5016, 9381, 5296, 14851, 2288, 8592, 45164, 25961, 2655, 29739, 4117, 2655, 13546, 25528, 19446, 43242, 11296, 14739, 11622, 32640, 16247, 2304, 26895, 31377, 1829, 4724, 4587, 7578, 25961, 2655, 3671, 24976, 10721, 2304, 26895, 31377], "avg_logprob": -0.21373279488414798, "compression_ratio": 1.956896551724138, "no_speech_prob": 2.384185791015625e-07, "words": [{"start": 821.57, 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"seek": 112993, "start": 1106.49, "end": 1129.93, "text": "هك منكون خلصنا أول فحص اللى هو الـomyelase هنتقل للجيلاتيناز هنتكلم عن تانى أنزايم اللى هو الجيلاتيناز الجيلاتيناز يعتبر exo enzyme ال substrate اللى هيشتغل عليها الجيلاتين", "tokens": [3224, 4117, 3714, 1863, 30544, 16490, 1211, 9381, 8315, 5551, 12610, 6156, 5016, 9381, 13672, 7578, 31439, 2423, 39184, 8488, 338, 651, 8032, 29399, 4587, 1211, 24976, 7435, 26895, 9307, 9957, 31377, 8032, 29399, 4117, 19528, 18871, 6055, 7649, 7578, 14739, 11622, 995, 32640, 13672, 7578, 31439, 25724, 26895, 9307, 9957, 31377, 25724, 26895, 9307, 9957, 31377, 7251, 34268, 26890, 454, 78, 24521, 2423, 27585, 13672, 7578, 8032, 1829, 8592, 2655, 17082, 1211, 25894, 11296, 25724, 26895, 9307, 9957], "avg_logprob": -0.24492187704890966, "compression_ratio": 1.727810650887574, "no_speech_prob": 2.384185791015625e-07, "words": [{"start": 1106.49, "end": 1106.79, "word": "هك", "probability": 0.877197265625}, {"start": 1106.79, "end": 1107.21, "word": 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"temperature": 1.0}, {"id": 53, "seek": 137826, "start": 1359.5, "end": 1378.26, "text": "الستافيلو كوكاس أورياس جيلتان جيلاتيناز positive لكن الاستافيلو كوكاس ايه بدار مدرس تعتبر جيلاتيناز negative احنا قلنا انه الاستافيلو كوكاي بشكل عام الجنراهاي gram positive كوكاي كتلاز positive تمام", "tokens": [6027, 14851, 31845, 26895, 2407, 9122, 2407, 4117, 32277, 34051, 16572, 32277, 10874, 26895, 2655, 7649, 10874, 26895, 9307, 1829, 8315, 11622, 3353, 44381, 42963, 14851, 31845, 26895, 2407, 9122, 2407, 4117, 32277, 1975, 1829, 3224, 47525, 9640, 3714, 3215, 2288, 3794, 6055, 34268, 26890, 10874, 26895, 9307, 1829, 8315, 11622, 3671, 1975, 5016, 8315, 12174, 1211, 8315, 16472, 3224, 42963, 14851, 31845, 26895, 2407, 9122, 2407, 4117, 47302, 4724, 8592, 28820, 6225, 10943, 25724, 1863, 23557, 11296, 1829, 21353, 3353, 9122, 2407, 4117, 47302, 9122, 2655, 1211, 31377, 3353, 46811, 10943], "avg_logprob": -0.30309140297674364, "compression_ratio": 1.9415204678362572, "no_speech_prob": 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المقارنة هما تنتيني ground positive ككاي كتلز positive لـ Staphylococcus aureus لكن في end species لـ Staphylococcus aureus و لـ Staphylococcus epidermidis قلنا الـ Coagulase positive لـ Staphylococcus aureus و الجيلاتيناز positive", "tokens": [6027, 39184, 745, 569, 3495, 5842, 905, 1149, 257, 540, 301, 12174, 1211, 8315, 39896, 3100, 25473, 44381, 2423, 39184, 745, 569, 3495, 5842, 905, 1149, 13510, 966, 40920, 39896, 22902, 3142, 8978, 9122, 2304, 7649, 6156, 5016, 9381, 9957, 39896, 29973, 14407, 20449, 8315, 16490, 1211, 6027, 25124, 16572, 5016, 23758, 31439, 9673, 4587, 9640, 1863, 3660, 8032, 15042, 6055, 29399, 9957, 1829, 2727, 3353, 9122, 4117, 47302, 9122, 2655, 1211, 11622, 3353, 5296, 39184, 745, 569, 3495, 5842, 905, 1149, 257, 540, 301, 44381, 8978, 917, 6172, 5296, 39184, 745, 569, 3495, 5842, 905, 1149, 257, 540, 301, 4032, 5296, 39184, 745, 569, 3495, 5842, 905, 1149, 13510, 966, 40920, 12174, 1211, 8315, 2423, 39184, 3066, 559, 425, 651, 3353, 5296, 39184, 745, 569, 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1410.69, "end": 1411.47, "word": " epidermidis", "probability": 0.9007161458333334}, {"start": 1411.47, "end": 1411.73, "word": " هي", "probability": 0.78076171875}, {"start": 1411.73, "end": 1412.75, "word": " abortionistic", "probability": 0.674560546875}, {"start": 1412.75, "end": 1413.55, "word": " في", "probability": 0.595703125}, {"start": 1413.55, "end": 1413.99, "word": " كمان", "probability": 0.9280598958333334}, {"start": 1413.99, "end": 1414.57, "word": " فحصين", "probability": 0.9237060546875}, {"start": 1414.57, "end": 1415.07, "word": " هيمروا", "probability": 0.8878580729166666}, {"start": 1415.07, "end": 1415.41, "word": " معنا", "probability": 0.896484375}, {"start": 1415.41, "end": 1416.07, "word": " خلال", "probability": 0.98486328125}, {"start": 1416.07, "end": 1417.71, "word": " الشريح", "probability": 0.9366861979166666}, {"start": 1417.71, "end": 1418.37, "word": " هذا", "probability": 0.6357421875}, {"start": 1418.37, "end": 1418.73, "word": " هو", "probability": 0.94921875}, {"start": 1418.73, "end": 1419.67, "word": " المقارنة", "probability": 0.99267578125}, {"start": 1419.67, "end": 1419.93, "word": " هما", "probability": 0.76708984375}, {"start": 1419.93, "end": 1420.53, "word": " تنتيني", "probability": 0.49688720703125}, {"start": 1420.53, "end": 1420.75, "word": " ground", "probability": 0.308349609375}, {"start": 1420.75, "end": 1421.17, "word": " positive", "probability": 0.85693359375}, {"start": 1421.17, "end": 1421.69, "word": " ككاي", "probability": 0.59521484375}, {"start": 1421.69, "end": 1422.17, "word": " كتلز", "probability": 0.625335693359375}, {"start": 1422.17, "end": 1422.67, "word": " positive", "probability": 0.73974609375}, {"start": 1422.67, "end": 1422.89, "word": " لـ", "probability": 0.7099609375}, {"start": 1422.89, "end": 1423.61, "word": " Staphylococcus", "probability": 0.8727213541666666}, {"start": 1423.61, "end": 1423.75, "word": " aureus", "probability": 0.78076171875}, {"start": 1423.75, "end": 1424.31, "word": " لكن", "probability": 0.7646484375}, {"start": 1424.31, "end": 1424.55, "word": " في", "probability": 0.935546875}, {"start": 1424.55, "end": 1424.73, "word": " end", "probability": 0.09844970703125}, {"start": 1424.73, "end": 1425.29, "word": " species", "probability": 0.77978515625}, {"start": 1425.29, "end": 1425.65, "word": " لـ", "probability": 0.806396484375}, {"start": 1425.65, "end": 1426.31, "word": " Staphylococcus", "probability": 0.9668782552083334}, {"start": 1426.31, "end": 1426.83, "word": " aureus", "probability": 0.9627278645833334}, {"start": 1426.83, "end": 1426.97, "word": " و", "probability": 0.8759765625}, {"start": 1426.97, "end": 1427.11, "word": " لـ", "probability": 0.8447265625}, {"start": 1427.11, "end": 1427.85, "word": " Staphylococcus", "probability": 0.9774576822916666}, {"start": 1427.85, "end": 1428.73, "word": " epidermidis", "probability": 0.9763997395833334}, {"start": 1428.73, "end": 1429.11, "word": " قلنا", "probability": 0.9405924479166666}, {"start": 1429.11, "end": 1429.27, "word": " الـ", "probability": 0.5487060546875}, {"start": 1429.27, "end": 1429.89, "word": " Coagulase", "probability": 0.7706298828125}, {"start": 1429.89, "end": 1430.35, "word": " positive", "probability": 0.8388671875}, {"start": 1430.35, "end": 1430.59, "word": " لـ", "probability": 0.6630859375}, {"start": 1430.59, "end": 1431.33, "word": " Staphylococcus", "probability": 0.9724934895833334}, {"start": 1431.33, "end": 1431.85, "word": " aureus", "probability": 0.9674479166666666}, {"start": 1431.85, "end": 1432.01, "word": " و", "probability": 0.71435546875}, {"start": 1432.01, "end": 1432.79, "word": " الجيلاتيناز", "probability": 0.63662109375}, {"start": 1432.79, "end": 1433.25, "word": " positive", "probability": 0.76953125}], "temperature": 1.0}, {"id": 56, "seek": 144619, "start": 1433.87, "end": 1446.19, "text": "في عندي فحصين هنتعرف عليهم أو هيمروا معانا خلال الشباتر الجاي ال money toll و ال DNS برضه positive في الاستفالة الكوكس او yes لكن negative في الاستفالة الكوكس epidermis", "tokens": [41185, 18871, 16254, 6156, 5016, 9381, 9957, 8032, 29399, 3615, 28480, 25894, 16095, 34051, 8032, 32640, 2288, 14407, 20449, 7649, 995, 16490, 1211, 6027, 25124, 3555, 9307, 2288, 25724, 47302, 2423, 1460, 16629, 4032, 2423, 35153, 4724, 43042, 3224, 3353, 8978, 42963, 14851, 5172, 6027, 3660, 33251, 2407, 4117, 3794, 1975, 2407, 2086, 44381, 3671, 8978, 42963, 14851, 5172, 6027, 3660, 33251, 2407, 4117, 3794, 13510, 966, 271], "avg_logprob": -0.25203804520593176, "compression_ratio": 1.5344827586206897, "no_speech_prob": 1.7881393432617188e-07, "words": [{"start": 1433.87, "end": 1434.05, "word": "في", "probability": 0.76953125}, {"start": 1434.05, "end": 1434.27, "word": " عندي", "probability": 0.52337646484375}, {"start": 1434.27, "end": 1434.81, "word": " فحصين", "probability": 0.9429931640625}, {"start": 1434.81, "end": 1435.37, "word": " هنتعرف", "probability": 0.92822265625}, {"start": 1435.37, "end": 1435.73, "word": " عليهم", "probability": 0.966796875}, {"start": 1435.73, "end": 1435.89, "word": " أو", "probability": 0.71826171875}, {"start": 1435.89, "end": 1436.33, "word": " هيمروا", "probability": 0.8067626953125}, {"start": 1436.33, "end": 1436.61, "word": " معانا", "probability": 0.8207194010416666}, {"start": 1436.61, "end": 1437.19, "word": " خلال", "probability": 0.9944661458333334}, {"start": 1437.19, "end": 1438.23, "word": " الشباتر", "probability": 0.931884765625}, {"start": 1438.23, "end": 1438.77, "word": " الجاي", "probability": 0.86767578125}, {"start": 1438.77, "end": 1438.97, "word": " ال", "probability": 0.85888671875}, {"start": 1438.97, "end": 1439.17, "word": " money", "probability": 0.4736328125}, {"start": 1439.17, "end": 1439.51, "word": " toll", "probability": 0.407958984375}, {"start": 1439.51, "end": 1439.67, "word": " و", "probability": 0.52197265625}, {"start": 1439.67, "end": 1439.73, "word": " ال", "probability": 0.86767578125}, {"start": 1439.73, "end": 1440.27, "word": " DNS", "probability": 0.736328125}, {"start": 1440.27, "end": 1440.81, "word": " برضه", "probability": 0.9580078125}, {"start": 1440.81, "end": 1441.15, "word": " positive", "probability": 0.81884765625}, {"start": 1441.15, "end": 1441.33, "word": " في", "probability": 0.8837890625}, {"start": 1441.33, "end": 1441.81, "word": " الاستفالة", "probability": 0.715234375}, {"start": 1441.81, "end": 1442.13, "word": " الكوكس", "probability": 0.77392578125}, {"start": 1442.13, "end": 1442.43, "word": " او", "probability": 0.655517578125}, {"start": 1442.43, "end": 1442.69, "word": " yes", "probability": 0.73583984375}, {"start": 1442.69, "end": 1443.05, "word": " لكن", "probability": 0.8125}, {"start": 1443.05, "end": 1443.71, "word": " negative", "probability": 0.677734375}, {"start": 1443.71, "end": 1444.37, "word": " في", "probability": 0.96533203125}, {"start": 1444.37, "end": 1445.01, "word": " الاستفالة", "probability": 0.9779296875}, {"start": 1445.01, "end": 1445.51, "word": " الكوكس", "probability": 0.976806640625}, {"start": 1445.51, "end": 1446.19, "word": " epidermis", "probability": 0.5746256510416666}], "temperature": 1.0}, {"id": 57, "seek": 147119, "start": 1449.21, "end": 1471.19, "text": "Bacillus anthrax and Bacillus cereus and several other members of the genus are gelatinous positive as are Clostridium titani and Clostridium perifringus قلنا إن ال Clostridium و البacillus يعتبروا إجرام positive بسلائي Spore forming بفرق بينهم بفحص الكتلاز", "tokens": [33, 326, 373, 301, 25820, 424, 87, 293, 363, 326, 373, 301, 11643, 301, 293, 2940, 661, 2679, 295, 264, 1049, 301, 366, 45174, 563, 3353, 382, 366, 2033, 555, 8558, 2197, 3459, 3782, 293, 2033, 555, 8558, 2197, 680, 351, 2937, 301, 12174, 1211, 8315, 36145, 2423, 2033, 555, 8558, 2197, 4032, 29739, 326, 373, 301, 7251, 34268, 26890, 14407, 11933, 7435, 2288, 10943, 3353, 4724, 3794, 1211, 16373, 1829, 1738, 418, 15745, 4724, 5172, 2288, 4587, 49374, 16095, 4724, 5172, 5016, 9381, 33251, 2655, 1211, 31377], "avg_logprob": -0.3023174184091975, "compression_ratio": 1.4759615384615385, "no_speech_prob": 2.980232238769531e-07, "words": [{"start": 1449.21, "end": 1449.97, "word": "Bacillus", "probability": 0.7664794921875}, {"start": 1449.97, "end": 1450.79, "word": " anthrax", "probability": 0.8792317708333334}, {"start": 1450.79, "end": 1451.29, "word": " and", "probability": 0.80712890625}, {"start": 1451.29, "end": 1451.87, "word": " Bacillus", "probability": 0.798095703125}, {"start": 1451.87, "end": 1452.41, "word": " cereus", "probability": 0.812255859375}, {"start": 1452.41, "end": 1452.65, "word": " and", "probability": 0.9228515625}, {"start": 1452.65, "end": 1452.99, "word": " several", "probability": 0.89013671875}, {"start": 1452.99, "end": 1453.55, "word": " other", "probability": 0.85205078125}, {"start": 1453.55, "end": 1454.09, "word": " members", "probability": 0.6103515625}, {"start": 1454.09, "end": 1454.33, "word": " of", "probability": 0.98046875}, {"start": 1454.33, "end": 1455.35, "word": " the", "probability": 0.91357421875}, {"start": 1455.35, "end": 1455.91, "word": " genus", "probability": 0.91650390625}, {"start": 1455.91, "end": 1456.21, "word": " are", "probability": 0.7734375}, {"start": 1456.21, "end": 1456.99, "word": " gelatinous", "probability": 0.79541015625}, {"start": 1456.99, "end": 1457.57, "word": " positive", "probability": 0.75048828125}, {"start": 1457.57, "end": 1458.47, "word": " as", "probability": 0.471923828125}, {"start": 1458.47, "end": 1458.79, "word": " are", "probability": 0.9169921875}, {"start": 1458.79, "end": 1459.51, "word": " Clostridium", "probability": 0.906005859375}, {"start": 1459.51, "end": 1459.93, "word": " titani", "probability": 0.807373046875}, {"start": 1459.93, "end": 1460.37, "word": " and", "probability": 0.62451171875}, {"start": 1460.37, "end": 1461.25, "word": " Clostridium", "probability": 0.9725341796875}, {"start": 1461.25, "end": 1462.29, "word": " perifringus", "probability": 0.572265625}, {"start": 1462.29, "end": 1462.83, "word": " قلنا", "probability": 0.7296549479166666}, {"start": 1462.83, "end": 1463.13, "word": " إن", "probability": 0.4287109375}, {"start": 1463.13, "end": 1463.29, "word": " ال", "probability": 0.382568359375}, {"start": 1463.29, "end": 1464.07, "word": " Clostridium", "probability": 0.875244140625}, {"start": 1464.07, "end": 1464.19, "word": " و", "probability": 0.56689453125}, {"start": 1464.19, "end": 1464.75, "word": " البacillus", "probability": 0.6724853515625}, {"start": 1464.75, "end": 1465.41, "word": " يعتبروا", "probability": 0.92236328125}, {"start": 1465.41, "end": 1465.91, "word": " إجرام", "probability": 0.78851318359375}, {"start": 1465.91, "end": 1466.43, "word": " positive", "probability": 0.7373046875}, {"start": 1466.43, "end": 1467.63, "word": " بسلائي", "probability": 0.51402587890625}, {"start": 1467.63, "end": 1468.31, "word": " Spore", "probability": 0.58160400390625}, {"start": 1468.31, "end": 1468.93, "word": " forming", "probability": 0.6455078125}, {"start": 1468.93, "end": 1469.57, "word": " بفرق", "probability": 0.9029541015625}, {"start": 1469.57, "end": 1469.91, "word": " بينهم", "probability": 0.955078125}, {"start": 1469.91, "end": 1470.43, "word": " بفحص", "probability": 0.9794921875}, {"start": 1470.43, "end": 1471.19, "word": " الكتلاز", "probability": 0.890380859375}], "temperature": 1.0}, {"id": 58, "seek": 149444, "start": 1472.14, "end": 1494.44, "text": "البصيلة الـ Sirius والبصيلة الانتراكس بقولهم جيلاتيناز positive وكمان التنتان إذا بدنا نربط المعلومات كانوا يعتبروا central in the endospar الكلستريديوم تيتانيب والكلستريديوم بيرفرنجز برضه يعتبروا جيلاتيناز positive يبقى هذا هو ال significant للفحص", "tokens": [6027, 3555, 9381, 26895, 3660, 2423, 39184, 6144, 4872, 16070, 3555, 9381, 26895, 3660, 2423, 7649, 2655, 23557, 4117, 3794, 4724, 39648, 16095, 10874, 26895, 9307, 1829, 8315, 11622, 3353, 4032, 24793, 7649, 16712, 29399, 7649, 11933, 15730, 47525, 8315, 8717, 25513, 9566, 9673, 30241, 20498, 9307, 25961, 14407, 7251, 34268, 26890, 14407, 5777, 294, 264, 917, 329, 2181, 2423, 28820, 14851, 16572, 16254, 20498, 6055, 36081, 7649, 1829, 3555, 16070, 28820, 14851, 16572, 16254, 20498, 4724, 13546, 5172, 2288, 1863, 7435, 11622, 4724, 43042, 3224, 7251, 34268, 26890, 14407, 10874, 26895, 9307, 1829, 8315, 11622, 3353, 7251, 3555, 4587, 7578, 23758, 31439, 2423, 4776, 24976, 5172, 5016, 9381], "avg_logprob": -0.23267045617103577, "compression_ratio": 1.7672413793103448, "no_speech_prob": 3.5762786865234375e-07, "words": [{"start": 1472.14, "end": 1472.84, "word": "البصيلة", "probability": 0.709619140625}, {"start": 1472.84, "end": 1473.0, "word": " الـ", "probability": 0.61767578125}, {"start": 1473.0, "end": 1473.46, "word": " Sirius", "probability": 0.699462890625}, {"start": 1473.46, "end": 1474.12, "word": " والبصيلة", "probability": 0.92197265625}, {"start": 1474.12, "end": 1474.78, "word": " الانتراكس", "probability": 0.7119954427083334}, {"start": 1474.78, "end": 1475.5, "word": " بقولهم", "probability": 0.658203125}, {"start": 1475.5, "end": 1476.62, "word": " جيلاتيناز", "probability": 0.791259765625}, {"start": 1476.62, "end": 1477.14, "word": " positive", "probability": 0.77880859375}, {"start": 1477.14, "end": 1477.9, "word": " وكمان", "probability": 0.87939453125}, {"start": 1477.9, "end": 1478.74, "word": " التنتان", "probability": 0.6722005208333334}, {"start": 1478.74, "end": 1479.2, "word": " إذا", "probability": 0.868896484375}, {"start": 1479.2, "end": 1479.64, "word": " بدنا", "probability": 0.98046875}, {"start": 1479.64, "end": 1480.24, "word": " نربط", "probability": 0.9833984375}, {"start": 1480.24, "end": 1481.06, "word": " المعلومات", "probability": 0.9879150390625}, {"start": 1481.06, "end": 1481.36, "word": " كانوا", "probability": 0.959716796875}, {"start": 1481.36, "end": 1481.92, "word": " يعتبروا", "probability": 0.9671630859375}, {"start": 1481.92, "end": 1482.56, "word": " central", "probability": 0.57373046875}, {"start": 1482.56, "end": 1483.42, "word": " in", "probability": 0.86767578125}, {"start": 1483.42, "end": 1483.62, "word": " the", "probability": 0.66162109375}, {"start": 1483.62, "end": 1484.68, "word": " endospar", "probability": 0.5131022135416666}, {"start": 1484.68, "end": 1486.04, "word": " الكلستريديوم", "probability": 0.6923828125}, {"start": 1486.04, "end": 1486.6, "word": " تيتانيب", "probability": 0.83505859375}, {"start": 1486.6, "end": 1487.5, "word": " والكلستريديوم", "probability": 0.937744140625}, {"start": 1487.5, "end": 1488.38, "word": " بيرفرنجز", "probability": 0.6351143973214286}, {"start": 1488.38, "end": 1488.7, "word": " برضه", "probability": 0.9401041666666666}, {"start": 1488.7, "end": 1489.4, "word": " يعتبروا", "probability": 0.9637451171875}, {"start": 1489.4, "end": 1490.3, "word": " جيلاتيناز", "probability": 0.9615071614583334}, {"start": 1490.3, "end": 1490.76, "word": " 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عشرين درجة سليزيوس او اعلى يبقى الجيلاتين بتتغير حالته حسب درجة الحرارة وهي معلومة مهمة جدا و ال bacteria that produce the enzyme gelatinase are grown in a gelatin media the enzyme break up", "tokens": [9778, 2304, 3794, 3660, 4032, 6225, 46309, 9957, 11778, 47341, 3660, 8608, 20292, 11622, 1829, 41779, 10632, 1975, 2407, 1975, 4587, 1211, 4724, 1829, 30544, 6553, 5296, 15042, 6055, 30544, 11778, 47341, 3660, 21542, 2288, 9640, 3660, 8608, 2655, 3660, 4032, 6225, 46309, 9957, 11778, 47341, 3660, 8608, 20292, 11622, 1829, 41779, 1975, 2407, 1975, 3615, 23942, 7251, 3555, 4587, 7578, 25724, 26895, 9307, 9957, 39894, 2655, 17082, 13546, 11331, 6027, 47395, 11331, 35457, 11778, 47341, 3660, 21542, 2288, 9640, 3660, 37037, 1829, 20449, 1211, 20498, 3660, 3714, 16095, 3660, 10874, 28259, 4032, 2423, 11763, 300, 5258, 264, 24521, 45174, 651, 366, 7709, 294, 257, 45174, 3021, 264, 24521, 1821, 493], "avg_logprob": -0.1702008874022535, "compression_ratio": 1.75, "no_speech_prob": 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"no_speech_prob": 3.224611282348633e-05, "words": [{"start": 2478.73, "end": 2479.37, "word": "لأنه", "probability": 0.6424967447916666}, {"start": 2479.37, "end": 2479.65, "word": " اللي", "probability": 0.59765625}, {"start": 2479.65, "end": 2479.93, "word": " قلنا", "probability": 0.8660481770833334}, {"start": 2479.93, "end": 2480.07, "word": " في", "probability": 0.89111328125}, {"start": 2480.07, "end": 2480.35, "word": " بعض", "probability": 0.880126953125}, {"start": 2480.35, "end": 2480.67, "word": " أنواع", "probability": 0.9576822916666666}, {"start": 2480.67, "end": 2481.19, "word": " البكتيريا", "probability": 0.94716796875}, {"start": 2481.19, "end": 2481.77, "word": " بتفرز", "probability": 0.903564453125}, {"start": 2481.77, "end": 2482.45, "word": " الانزيم", "probability": 0.7882080078125}, {"start": 2482.45, "end": 2483.25, "word": " بكميات", "probability": 0.9508056640625}, {"start": 2483.25, "end": 2484.45, "word": " قليلة", "probability": 0.94921875}, {"start": 2484.45, "end": 2484.85, "word": " هذا", "probability": 0.8291015625}, {"start": 2484.85, "end": 2485.37, "word": " بالنسبة", "probability": 0.9874267578125}, {"start": 2485.37, "end": 2486.03, "word": " لطريقة", "probability": 0.9718017578125}, {"start": 2486.03, "end": 2486.27, "word": " ال", "probability": 0.8173828125}, {"start": 2486.27, "end": 2486.61, "word": " gelatin", "probability": 0.366455078125}, {"start": 2486.61, "end": 2487.33, "word": " strip", "probability": 0.82861328125}, {"start": 2487.33, "end": 2487.83, "word": " method", "probability": 0.96533203125}, {"start": 2487.83, "end": 2488.63, "word": " هي", "probability": 0.599609375}, {"start": 2488.63, "end": 2489.23, "word": " alternative", "probability": 0.79150390625}, {"start": 2489.23, "end": 2489.87, "word": " method", "probability": 0.97607421875}, {"start": 2489.87, "end": 2490.17, "word": " for", "probability": 0.92578125}, {"start": 2490.17, "end": 2490.81, "word": " detecting", "probability": 0.779296875}, {"start": 2490.81, "end": 2491.71, "word": " gelatinase", "probability": 0.880615234375}, {"start": 2491.71, "end": 2492.35, "word": " production", "probability": 0.94873046875}, {"start": 2492.35, "end": 2492.87, "word": " طريقة", "probability": 0.9630533854166666}, {"start": 2492.87, "end": 2493.17, "word": " تانية", "probability": 0.9879557291666666}, {"start": 2493.17, "end": 2493.41, "word": " عشان", "probability": 0.9734700520833334}, {"start": 2493.41, "end": 2494.05, "word": " أفحص", "probability": 0.8382568359375}, {"start": 2494.05, "end": 2494.27, "word": " عن", "probability": 0.96875}, {"start": 2494.27, "end": 2495.19, "word": " الجيلاتيناز", "probability": 0.7830403645833334}, {"start": 2495.19, "end": 2496.15, "word": " باستخدم", "probability": 0.834765625}, {"start": 2496.15, "end": 2496.31, "word": " ال", "probability": 0.9189453125}, {"start": 2496.31, "end": 2496.61, "word": " X", "probability": 0.78857421875}, {"start": 2496.61, "end": 2496.95, "word": "-ray", 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"probability": 0.9890950520833334}, {"start": 2519.5, "end": 2519.78, "word": " من", "probability": 0.99609375}, {"start": 2519.78, "end": 2520.02, "word": " ال", "probability": 0.94873046875}, {"start": 2520.02, "end": 2520.64, "word": " strip", "probability": 0.888671875}, {"start": 2520.64, "end": 2520.98, "word": " كف", "probability": 0.65283203125}, {"start": 2520.98, "end": 2521.44, "word": " الطريقة", "probability": 0.760498046875}, {"start": 2521.44, "end": 2521.64, "word": " زي", "probability": 0.954833984375}, {"start": 2521.64, "end": 2521.72, "word": " ما", "probability": 0.9912109375}, {"start": 2521.72, "end": 2522.0, "word": " قلنا", "probability": 0.89599609375}, {"start": 2522.0, "end": 2522.4, "word": " بعمل", "probability": 0.951171875}, {"start": 2522.4, "end": 2523.0, "word": " انكولات", "probability": 0.696044921875}, {"start": 2523.0, "end": 2524.12, "word": " للبكتيريا", "probability": 0.8614501953125}, {"start": 2524.12, "end": 2524.96, "word": " في", "probability": 0.94677734375}, {"start": 2524.96, "end": 2525.42, "word": " نص", "probability": 0.983642578125}, {"start": 2525.42, "end": 2525.68, "word": " مل", "probability": 0.757568359375}, {"start": 2525.68, "end": 2525.9, "word": " من", "probability": 0.99169921875}, {"start": 2525.9, "end": 2526.08, "word": " ال", "probability": 0.9150390625}, {"start": 2526.08, "end": 2526.34, "word": " normal", "probability": 0.354248046875}, {"start": 2526.34, "end": 2526.94, "word": " saline", "probability": 0.905517578125}], "temperature": 1.0}, {"id": 99, "seek": 255127, "start": 2527.77, "end": 2551.27, "text": "لازم يكون ال suspension تربط بعدين بحط ال X-ray film أو الجلاتين في ال bacterial suspension أو ال saline suspension بعمل incubation لمدة أربع ساعات لأربع عشرين ساعة عشان ألاحظ إزالة اللي هو الجلاتين emulsion من ال strip من اللاحظ في هذه الصورة", "tokens": [1211, 31377, 2304, 7251, 30544, 2423, 15771, 6055, 25513, 9566, 39182, 9957, 4724, 5016, 9566, 2423, 1783, 12, 3458, 2007, 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{"start": 2529.79, "end": 2530.13, "word": " بعدين", "probability": 0.6083984375}, {"start": 2530.13, "end": 2530.53, "word": " بحط", "probability": 0.951171875}, {"start": 2530.53, "end": 2530.67, "word": " ال", "probability": 0.8837890625}, {"start": 2530.67, "end": 2530.95, "word": " X", "probability": 0.392333984375}, {"start": 2530.95, "end": 2531.19, "word": "-ray", "probability": 0.784423828125}, {"start": 2531.19, "end": 2531.55, "word": " film", "probability": 0.75634765625}, {"start": 2531.55, "end": 2531.77, "word": " أو", "probability": 0.6455078125}, {"start": 2531.77, "end": 2532.49, "word": " الجلاتين", "probability": 0.74853515625}, {"start": 2532.49, "end": 2532.89, "word": " في", "probability": 0.7548828125}, {"start": 2532.89, "end": 2533.71, "word": " ال", "probability": 0.41015625}, {"start": 2533.71, "end": 2535.01, "word": " bacterial", "probability": 0.3828125}, {"start": 2535.01, "end": 2535.61, "word": " suspension", "probability": 0.98583984375}, {"start": 2535.61, "end": 2535.85, "word": " أو", "probability": 0.5537109375}, {"start": 2535.85, "end": 2535.99, "word": " ال", "probability": 0.6669921875}, {"start": 2535.99, "end": 2536.33, "word": " saline", "probability": 0.886962890625}, {"start": 2536.33, "end": 2536.89, "word": " suspension", "probability": 0.99267578125}, {"start": 2536.89, "end": 2537.69, "word": " بعمل", "probability": 0.8850911458333334}, {"start": 2537.69, "end": 2538.29, "word": " incubation", "probability": 0.864990234375}, {"start": 2538.29, "end": 2538.83, "word": " لمدة", "probability": 0.961669921875}, {"start": 2538.83, "end": 2539.67, "word": " أربع", "probability": 0.7969563802083334}, {"start": 2539.67, "end": 2540.17, "word": " ساعات", "probability": 0.9475911458333334}, {"start": 2540.17, "end": 2540.61, "word": " لأربع", "probability": 0.80926513671875}, {"start": 2540.61, "end": 2541.15, "word": " عشرين", "probability": 0.8595377604166666}, {"start": 2541.15, "end": 2541.73, "word": " ساعة", "probability": 0.9728190104166666}, {"start": 2541.73, "end": 2542.97, "word": " عشان", "probability": 0.9581705729166666}, {"start": 2542.97, "end": 2543.55, "word": " ألاحظ", "probability": 0.9522705078125}, {"start": 2543.55, "end": 2544.21, "word": " إزالة", "probability": 0.92138671875}, {"start": 2544.21, "end": 2544.43, "word": " اللي", "probability": 0.662841796875}, {"start": 2544.43, "end": 2544.85, "word": " هو", "probability": 0.97998046875}, {"start": 2544.85, "end": 2545.69, "word": " الجلاتين", "probability": 0.910400390625}, {"start": 2545.69, "end": 2546.15, "word": " emulsion", "probability": 0.684814453125}, {"start": 2546.15, "end": 2546.53, "word": " من", "probability": 0.98974609375}, {"start": 2546.53, "end": 2546.83, "word": " ال", "probability": 0.8681640625}, {"start": 2546.83, "end": 2547.23, "word": " strip", "probability": 0.74267578125}, {"start": 2547.23, "end": 2549.67, "word": " من", "probability": 0.317626953125}, {"start": 2549.67, "end": 2550.11, "word": " اللاحظ", "probability": 0.8474934895833334}, {"start": 2550.11, "end": 2550.27, "word": " في", "probability": 0.94580078125}, {"start": 2550.27, "end": 2550.55, "word": " هذه", "probability": 0.74462890625}, {"start": 2550.55, "end": 2551.27, "word": " الصورة", "probability": 0.9913736979166666}], "temperature": 1.0}, {"id": 100, "seek": 256737, "start": 2553.09, "end": 2567.37, "text": "ما اختفى اللون الأسود من الـ Strip يبقى هذه النتيجة Negative للجيلاتيناز لكن هنا نلاحظ أن الـ Strip راح اللون الأسود منه يبقى هذه جيلاتيناز Positive", "tokens": [15042, 1975, 46456, 5172, 7578, 13672, 11536, 16247, 3794, 23328, 9154, 2423, 39184, 745, 8400, 7251, 3555, 4587, 7578, 29538, 28239, 31371, 7435, 3660, 43230, 24976, 7435, 26895, 9307, 1829, 8315, 11622, 44381, 34105, 8717, 15040, 5016, 19913, 14739, 2423, 39184, 745, 8400, 12602, 39319, 13672, 11536, 16247, 3794, 23328, 9154, 3224, 7251, 3555, 4587, 7578, 29538, 10874, 26895, 9307, 1829, 8315, 11622, 46326], "avg_logprob": 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في", "probability": 0.98779296875}, {"start": 2639.22, "end": 2639.48, "word": " كل", "probability": 0.9931640625}, {"start": 2639.48, "end": 2640.0, "word": " تيوب", "probability": 0.7960611979166666}], "temperature": 1.0}, {"id": 104, "seek": 266861, "start": 2640.19, "end": 2668.61, "text": "هيتغير لون التيوب لأ اللون الأسود يبقى نتيجة positive لأن البكتيريا عندها انزيم الجيلاتيناز كسر الجيلاتين الجيلاتين هو اللي بيخلّي الفحم متماسك طب الجيلاتين اتكسر راح يبقى الفحم هينتشر في كل تيوب وهيصير لونه أسود يبقى النتيجة ال positive distribution هيصير في يدي لون أسود في كل تيوب لكن نتيجة ال negative مش هتفرز انزيم الجيلاتيناز يبقى الجيلاتين هيضله", "tokens": [3224, 36081, 17082, 13546, 5296, 11536, 16712, 1829, 37746, 5296, 10721, 13672, 11536, 16247, 3794, 23328, 7251, 3555, 4587, 7578, 8717, 31371, 7435, 3660, 3353, 5296, 33456, 29739, 4117, 2655, 13546, 25528, 43242, 11296, 16472, 11622, 32640, 25724, 26895, 9307, 1829, 8315, 11622, 9122, 3794, 2288, 25724, 26895, 9307, 9957, 25724, 26895, 9307, 9957, 31439, 13672, 1829, 4724, 1829, 9778, 1211, 11703, 1829, 27188, 35571, 44650, 15042, 3794, 4117, 23032, 3555, 25724, 26895, 9307, 9957, 1975, 2655, 4117, 3794, 2288, 12602, 39319, 7251, 3555, 4587, 7578, 27188, 35571, 8032, 9957, 2655, 46309, 8978, 28242, 6055, 1829, 37746, 4032, 3224, 1829, 9381, 13546, 5296, 11536, 3224, 5551, 3794, 23328, 7251, 3555, 4587, 7578, 28239, 31371, 7435, 3660, 2423, 3353, 7316, 39896, 9381, 13546, 8978, 7251, 16254, 5296, 11536, 5551, 3794, 23328, 8978, 28242, 6055, 1829, 37746, 44381, 8717, 31371, 7435, 3660, 2423, 3671, 37893, 8032, 2655, 5172, 2288, 11622, 16472, 11622, 32640, 25724, 26895, 9307, 1829, 8315, 11622, 7251, 3555, 4587, 7578, 25724, 26895, 9307, 9957, 39896, 11242, 1211, 3224], "avg_logprob": -0.16443015328224966, "compression_ratio": 2.309433962264151, "no_speech_prob": 1.7821788787841797e-05, "words": [{"start": 2640.19, "end": 2640.81, "word": "هيتغير", "probability": 0.9290771484375}, {"start": 2640.81, "end": 2641.07, "word": " لون", "probability": 0.75439453125}, {"start": 2641.07, "end": 2641.47, "word": " التيوب", "probability": 0.7071126302083334}, {"start": 2641.47, "end": 2641.73, "word": " لأ", "probability": 0.8681640625}, {"start": 2641.73, "end": 2642.03, "word": " اللون", "probability": 0.75}, {"start": 2642.03, "end": 2642.49, "word": " الأسود", "probability": 0.9348958333333334}, {"start": 2642.49, "end": 2642.71, "word": " يبقى", "probability": 0.944091796875}, {"start": 2642.71, "end": 2643.23, "word": " نتيجة", "probability": 0.9708251953125}, {"start": 2643.23, "end": 2643.73, "word": " positive", "probability": 0.72021484375}, {"start": 2643.73, "end": 2644.79, "word": " لأن", "probability": 0.9267578125}, {"start": 2644.79, "end": 2646.35, "word": " البكتيريا", "probability": 0.87978515625}, {"start": 2646.35, "end": 2646.69, "word": " عندها", "probability": 0.982177734375}, {"start": 2646.69, "end": 2646.99, "word": " انزيم", "probability": 0.7500813802083334}, {"start": 2646.99, "end": 2647.77, "word": " الجيلاتيناز", "probability": 0.795654296875}, {"start": 2647.77, "end": 2648.31, "word": " كسر", "probability": 0.87939453125}, {"start": 2648.31, "end": 2649.07, "word": " الجيلاتين", "probability": 0.9349365234375}, {"start": 2649.07, "end": 2650.07, "word": " الجيلاتين", "probability": 0.918701171875}, {"start": 2650.07, "end": 2650.37, "word": " هو", "probability": 0.97802734375}, {"start": 2650.37, "end": 2650.53, "word": " اللي", "probability": 0.91943359375}, {"start": 2650.53, "end": 2650.95, "word": " بيخلّي", "probability": 0.7575887044270834}, {"start": 2650.95, "end": 2651.31, "word": " الفحم", "probability": 0.96435546875}, {"start": 2651.31, "end": 2652.11, "word": " متماسك", "probability": 0.921142578125}, {"start": 2652.11, "end": 2652.41, "word": " طب", "probability": 0.82470703125}, {"start": 2652.41, "end": 2653.07, "word": " الجيلاتين", "probability": 0.9642333984375}, {"start": 2653.07, "end": 2653.85, "word": " اتكسر", "probability": 0.94287109375}, {"start": 2653.85, "end": 2654.13, "word": " راح", "probability": 0.90966796875}, {"start": 2654.13, "end": 2654.61, "word": " يبقى", "probability": 0.9844970703125}, {"start": 2654.61, "end": 2655.07, "word": " الفحم", "probability": 0.9892578125}, {"start": 2655.07, "end": 2655.69, "word": " هينتشر", "probability": 0.84747314453125}, {"start": 2655.69, "end": 2655.87, "word": " في", "probability": 0.91162109375}, {"start": 2655.87, "end": 2656.11, "word": " كل", "probability": 0.99365234375}, {"start": 2656.11, "end": 2656.47, "word": " تيوب", "probability": 0.9415690104166666}, {"start": 2656.47, "end": 2656.91, "word": " وهيصير", "probability": 0.56376953125}, {"start": 2656.91, "end": 2657.37, "word": " لونه", "probability": 0.9617513020833334}, {"start": 2657.37, "end": 2657.73, "word": " أسود", "probability": 0.9807942708333334}, {"start": 2657.73, "end": 2657.91, "word": " يبقى", "probability": 0.9677734375}, {"start": 2657.91, "end": 2658.29, "word": " النتيجة", "probability": 0.8988037109375}, {"start": 2658.29, "end": 2658.41, "word": " ال", "probability": 0.525390625}, {"start": 2658.41, "end": 2658.75, "word": " positive", "probability": 0.8603515625}, {"start": 2658.75, "end": 2660.17, "word": " distribution", "probability": 0.7099609375}, {"start": 2660.17, "end": 2661.19, "word": " هيصير", "probability": 0.8580729166666666}, {"start": 2661.19, "end": 2661.33, "word": " في", "probability": 0.84814453125}, {"start": 2661.33, "end": 2661.53, "word": " يدي", "probability": 0.3612060546875}, {"start": 2661.53, "end": 2661.71, "word": " لون", "probability": 0.946533203125}, {"start": 2661.71, "end": 2662.03, "word": " أسود", "probability": 0.9822591145833334}, {"start": 2662.03, "end": 2662.21, "word": " في", "probability": 0.9765625}, {"start": 2662.21, "end": 2662.43, "word": " كل", "probability": 0.99365234375}, {"start": 2662.43, "end": 2662.83, "word": " تيوب", "probability": 0.9694010416666666}, {"start": 2662.83, "end": 2663.33, "word": " لكن", "probability": 0.93896484375}, {"start": 2663.33, "end": 2664.05, "word": " نتيجة", "probability": 0.9796142578125}, {"start": 2664.05, "end": 2664.15, "word": " ال", "probability": 0.732421875}, {"start": 2664.15, "end": 2664.41, "word": " negative", "probability": 0.3525390625}, {"start": 2664.41, "end": 2665.15, "word": " مش", "probability": 0.8662109375}, {"start": 2665.15, "end": 2665.81, "word": " هتفرز", "probability": 0.95439453125}, {"start": 2665.81, "end": 2666.23, "word": " انزيم", "probability": 0.87109375}, {"start": 2666.23, "end": 2667.07, "word": " الجيلاتيناز", "probability": 0.947509765625}, {"start": 2667.07, "end": 2667.39, "word": " يبقى", "probability": 0.9615478515625}, {"start": 2667.39, "end": 2667.93, "word": " الجيلاتين", "probability": 0.9703369140625}, {"start": 2667.93, "end": 2668.61, "word": " هيضله", "probability": 0.6444091796875}], "temperature": 1.0}, {"id": 105, "seek": 269307, "start": 2669.15, "end": 2693.07, "text": "بين جزيئات الفحم فبالتالي هيترسب في القاهة هذا فكرة let's share cool هكذا خلصنا طحس الجيلاتيناز وخلصنا ال chapter اللي كان بيتكلم عن الاميلاز and جيلاتيناز وقلنا اتنين exoenzyme بتمنى تكون الأمور واضحة والسلام عليكم ورحمة الله وبركاته", "tokens": [3555, 9957, 10874, 11622, 1829, 19986, 9307, 27188, 35571, 6156, 3555, 6027, 2655, 6027, 1829, 39896, 2655, 2288, 35457, 8978, 25062, 40294, 3660, 23758, 6156, 4117, 25720, 718, 311, 2073, 1627, 8032, 4117, 15730, 16490, 1211, 9381, 8315, 23032, 5016, 3794, 25724, 26895, 9307, 1829, 8315, 11622, 4032, 9778, 1211, 9381, 8315, 2423, 7187, 13672, 1829, 25961, 4724, 36081, 4117, 19528, 18871, 2423, 10943, 1829, 15040, 11622, 293, 10874, 26895, 9307, 1829, 8315, 11622, 4032, 4587, 1211, 8315, 1975, 2655, 1863, 9957, 454, 78, 268, 1229, 1398, 4724, 39237, 1863, 7578, 6055, 30544, 16247, 2304, 13063, 4032, 46958, 5016, 3660, 16070, 3794, 37440, 25894, 24793, 4032, 2288, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224], "avg_logprob": -0.25228987480032034, "compression_ratio": 1.6583333333333334, "no_speech_prob": 3.55839729309082e-05, "words": [{"start": 2669.15, "end": 2669.53, "word": "بين", "probability": 0.706787109375}, {"start": 2669.53, "end": 2670.25, "word": " جزيئات", "probability": 0.876953125}, {"start": 2670.25, "end": 2670.89, "word": " الفحم", "probability": 0.989501953125}, {"start": 2670.89, "end": 2671.99, "word": " فبالتالي", "probability": 0.9677734375}, {"start": 2671.99, "end": 2672.71, "word": " هيترسب", "probability": 0.725830078125}, {"start": 2672.71, "end": 2673.07, "word": " في", "probability": 0.8525390625}, {"start": 2673.07, "end": 2673.71, "word": " القاهة", "probability": 0.8160807291666666}, {"start": 2673.71, "end": 2673.97, "word": " هذا", "probability": 0.369384765625}, {"start": 2673.97, "end": 2674.37, "word": " فكرة", "probability": 0.9674479166666666}, {"start": 2674.37, "end": 2674.79, "word": " let's", "probability": 0.3316650390625}, {"start": 2674.79, "end": 2675.03, "word": " share", "probability": 0.483642578125}, {"start": 2675.03, "end": 2675.55, "word": " cool", "probability": 0.61083984375}, {"start": 2675.55, "end": 2677.91, "word": " هكذا", "probability": 0.6947428385416666}, {"start": 2677.91, "end": 2678.91, "word": " خلصنا", "probability": 0.78302001953125}, {"start": 2678.91, "end": 2679.73, "word": " طحس", "probability": 0.760986328125}, {"start": 2679.73, "end": 2680.63, "word": " الجيلاتيناز", "probability": 0.7357584635416666}, {"start": 2680.63, "end": 2681.23, "word": " وخلصنا", "probability": 0.932421875}, {"start": 2681.23, "end": 2681.41, "word": " ال", "probability": 0.52197265625}, {"start": 2681.41, "end": 2681.73, "word": " chapter", "probability": 0.83984375}, {"start": 2681.73, "end": 2681.95, "word": " اللي", "probability": 0.73779296875}, {"start": 2681.95, "end": 2682.15, "word": " كان", "probability": 0.91943359375}, {"start": 2682.15, "end": 2682.63, "word": " بيتكلم", "probability": 0.82244873046875}, {"start": 2682.63, "end": 2682.95, "word": " عن", "probability": 0.9833984375}, {"start": 2682.95, "end": 2683.79, "word": " الاميلاز", "probability": 0.61337890625}, {"start": 2683.79, "end": 2684.07, "word": " and", "probability": 0.22607421875}, {"start": 2684.07, "end": 2684.77, "word": " جيلاتيناز", "probability": 0.8933919270833334}, {"start": 2684.77, "end": 2685.11, "word": " وقلنا", "probability": 0.8934326171875}, {"start": 2685.11, "end": 2685.65, "word": " اتنين", "probability": 0.655792236328125}, {"start": 2685.65, "end": 2687.87, "word": " exoenzyme", "probability": 0.796875}, {"start": 2687.87, "end": 2688.73, "word": " بتمنى", "probability": 0.819580078125}, {"start": 2688.73, "end": 2689.15, "word": " تكون", "probability": 0.93310546875}, {"start": 2689.15, "end": 2689.67, "word": " الأمور", "probability": 0.96484375}, {"start": 2689.67, "end": 2690.27, "word": " واضحة", "probability": 0.985107421875}, {"start": 2690.27, "end": 2690.79, "word": " والسلام", "probability": 0.8497721354166666}, {"start": 2690.79, "end": 2691.43, "word": " عليكم", "probability": 0.946533203125}, {"start": 2691.43, "end": 2691.97, "word": " ورحمة", "probability": 0.9844970703125}, {"start": 2691.97, "end": 2692.27, "word": " الله", "probability": 0.966796875}, {"start": 2692.27, "end": 2693.07, "word": " وبركاته", "probability": 0.97451171875}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 2694.8150625, "duration_after_vad": 2615.5881249999884}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/YZtUaTNI_xw_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/YZtUaTNI_xw_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..15b9b743d33ad43753329bf014deb8af5a3993e1
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/YZtUaTNI_xw_postprocess.srt
@@ -0,0 +1,2060 @@
+1
+00:00:01,960 --> 00:00:05,640
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:05,640 --> 00:00:09,080
+جميعًا في هذا اللقاء هنتكلم عن Catalase and
+
+3
+00:00:09,080 --> 00:00:13,620
+Coagulase Test قولنا إن البكتيريا Small and
+
+4
+00:00:13,620 --> 00:00:17,300
+Colorless اتغلبنا على إنها Small باستخدام
+
+5
+00:00:17,300 --> 00:00:20,520
+الميكروسكوب الميكروسكوب عملّي magnification
+
+6
+00:00:20,520 --> 00:00:25,320
+للبكتيريا وإقدرت إني أنا أشوفها بالنسبة لإنها
+
+7
+00:00:25,320 --> 00:00:30,080
+Colorless شفافة حلّت هذه المشكلة باستخدام الستان
+
+8
+00:00:30,080 --> 00:00:33,920
+باستخدام الصبغاطبتعرفنا على أنواع الصبغات اللي من
+
+9
+00:00:33,920 --> 00:00:38,360
+ضمنها لـ Gram Stain لـ Gram Stain كانت أول خطوة من
+
+10
+00:00:38,360 --> 00:00:42,180
+خطوات ال identification أول خطوة من خطوات تعريف
+
+11
+00:00:42,180 --> 00:00:47,320
+البكتيريا لـ Gram Stain قسمت البكتيريا لمجموعات لـ
+
+12
+00:00:47,320 --> 00:00:51,020
+Gram Positive Bacteria و Gram Negative Bacteria لـ
+
+13
+00:00:51,020 --> 00:00:55,100
+Gram Positive Bacteria بتضم مجموعة من البكتيريا و
+
+14
+00:00:55,100 --> 00:00:58,980
+كذلك لـ Gram Negative عشان أوصل للتعريف النهائي
+
+15
+00:00:59,210 --> 00:01:04,030
+لازم اعمل فحوصات كيميائية Biochemical Test واليوم
+
+16
+00:01:04,030 --> 00:01:09,130
+هنتكلم عن اول فحصين كيميائيين يهم ال Catalase and
+
+17
+00:01:09,130 --> 00:01:14,670
+Coagulase Test عشان نوضح فكرة الفحوصات الكيميائية
+
+18
+00:01:14,670 --> 00:01:20,030
+لو انت بدك تقابل بنت لأول مرة وعرفتي مواصفات هذه
+
+19
+00:01:20,030 --> 00:01:24,130
+البنت اكيد لما توصلي للمكان اللي هتتقابله فيه
+
+20
+00:01:24,130 --> 00:01:27,350
+وقتها تشوفي مين في المكان ليه نفس هذه المواصفات
+
+21
+00:01:27,350 --> 00:01:33,670
+وتستفنيلما توصلى لابنت اللى انت بدك اياها يبقى
+
+22
+00:01:33,670 --> 00:01:37,990
+جمعتى اكتر من صفة لهذه البنت عشان تعرفيها نفس
+
+23
+00:01:37,990 --> 00:01:41,950
+الشغل مع البكتيريا عشان اوصل للتعريف النهائى بدي
+
+24
+00:01:41,950 --> 00:01:46,550
+اجمع صفات وخصائص عشان اعرفها من خلال الفحوصات
+
+25
+00:01:46,550 --> 00:01:51,510
+الكيميائية شوف فكرة الفحوصات الكيميائية كل بكتيريا
+
+26
+00:01:51,510 --> 00:01:55,610
+عندها انزيمات معينة انا بدي اكشف هذه الانزيمات
+
+27
+00:01:55,610 --> 00:02:00,170
+موجودة او لأاحنا بنعرف إنه الانزيم بيشتغل على
+
+28
+00:02:00,170 --> 00:02:05,090
+substrate عشان أكشف هل الانزيم موجودة إن البكتيريا
+
+29
+00:02:05,090 --> 00:02:10,210
+أو لأ بجيب مادة كيميائية أقلد و أحاكي فيها ال
+
+30
+00:02:10,210 --> 00:02:13,090
+substrate اللي بيشتغل عليها الانزيم الموجود
+
+31
+00:02:13,090 --> 00:02:17,750
+بالبكتيريا ونستدل طبعا إن التفاعل صار أو لأ من
+
+32
+00:02:17,750 --> 00:02:22,230
+خلال نواتج التفاعل ممكن أشوف نواتج التفاعل بال
+
+33
+00:02:22,230 --> 00:02:28,290
+next I أو ممكن يكون تغير في لون ال mediaاليوم ان
+
+34
+00:02:28,290 --> 00:02:32,730
+شاء الله هنبدأ بأول فحص كيميائي الـ catalase test
+
+35
+00:02:32,730 --> 00:02:37,530
+لكن قبل ما نتكلم عن ال catalase and coagulase test
+
+36
+00:02:37,530 --> 00:02:46,870
+هنتكلم عن ال enzyme بشكل عام enzymes
+
+37
+00:02:46,870 --> 00:02:50,830
+in the microbiology lab biochemical test relies on
+
+38
+00:02:50,830 --> 00:02:55,100
+enzymeفي معمل الـ Microbiology الـ biochemical
+
+39
+00:02:55,100 --> 00:02:59,340
+test الفحوصات بتعتمد على الانزيمات which is
+
+40
+00:02:59,340 --> 00:03:04,120
+glycoprotein or protein that as catalyst by
+
+41
+00:03:04,120 --> 00:03:07,000
+lowering the activation energy of certain
+
+42
+00:03:07,000 --> 00:03:11,660
+biological reaction فيها تعريف مهم اللي هو ال
+
+43
+00:03:11,660 --> 00:03:16,570
+enzymeمعرف للإنزايم اللى هو ممكن انها تكون الـ
+
+44
+00:03:16,570 --> 00:03:21,590
+glycoprotein أو البروتين يبقى الإنزيمات ممكن تكون
+
+45
+00:03:21,590 --> 00:03:27,510
+ال glycoprotein بروتين مرتبط بوليغوسكاريد بشوجر أو
+
+46
+00:03:27,510 --> 00:03:33,090
+بروتين أو بروتين لحال بيشتغل as catalyst كمحفظ
+
+47
+00:03:34,880 --> 00:03:39,600
+بيشتغل كمحفز بيقلل طاقة التنشيط Lowering the
+
+48
+00:03:39,600 --> 00:03:43,760
+activation energy طيب لو بدنا نعرف طاقة التنشيط شو
+
+49
+00:03:43,760 --> 00:03:47,880
+هي ال activation energy طاقة التنشيط هو الحد
+
+50
+00:03:47,880 --> 00:03:52,620
+الأدنى من الطاقة اللازم لحدوث التفاعل يبقى أقل شئ
+
+51
+00:03:52,620 --> 00:03:56,600
+من الطاقة أنا بديه عشان يصير التفاعل بسميه ال
+
+52
+00:03:56,600 --> 00:04:01,810
+activation energyبالنسبة للمعادلة قلنا الإنزايم
+
+53
+00:04:01,810 --> 00:04:05,190
+هيشتغل على substrate ال substrate طبعا هي مواد
+
+54
+00:04:05,190 --> 00:04:10,290
+التفاعل عشان يعطيني product نستطيع استخدام علمنا
+
+55
+00:04:10,290 --> 00:04:15,870
+في الانزايم البكتيريا لنعرف البكتيريا ونقرر بين
+
+56
+00:04:15,870 --> 00:04:20,300
+مجموعات البكتيرياهدفنا من دراسة الإنزيمات
+
+57
+00:04:20,300 --> 00:04:24,420
+بالبكتيريا عشان أعمل identification و أميز
+
+58
+00:04:24,420 --> 00:04:29,260
+البكتيريا عن بعض زي ما وضحنا في بداية المحاضرة
+
+59
+00:04:29,260 --> 00:04:36,220
+types
+
+60
+00:04:36,220 --> 00:04:41,280
+of enzyme أنواع الإنزيمات الأنواع الإنزيمات تنقسم
+
+61
+00:04:41,280 --> 00:04:47,440
+حسبAccording to site of the reaction حسب موقع
+
+62
+00:04:47,440 --> 00:04:53,920
+العمل وحسب مكان أو حسب الإنتاج يبقى بقسم الإنزيمات
+
+63
+00:04:53,920 --> 00:04:57,780
+حسب موقع العمل ال site of reaction أو حسب ال
+
+64
+00:04:57,780 --> 00:05:03,440
+enzyme production حسب الإنتاج بالنسبة لموقع العمل
+
+65
+00:05:03,440 --> 00:05:08,580
+ال site of reaction بصنفها إلى endoenzyme and
+
+66
+00:05:08,580 --> 00:05:13,860
+exoenzyme يبقىالإنزيمات تنقسم على حسب ال site of
+
+67
+00:05:13,860 --> 00:05:18,820
+reaction إلى endoenzyme and exoenzyme بالنسبة لها
+
+68
+00:05:18,820 --> 00:05:22,840
+الـ endoenzyme بيصير التفاعل intracellular يبقى
+
+69
+00:05:22,840 --> 00:05:27,980
+بيصير التفاعل داخل الخلية where substrate and
+
+70
+00:05:27,980 --> 00:05:33,150
+enzyme react inside the cellبقول لي إن ال
+
+71
+00:05:33,150 --> 00:05:36,410
+substrate و ال enzyme هيتفاعلوا داخل الخلية ال
+
+72
+00:05:36,410 --> 00:05:41,150
+enzyme يفرز داخل الخلية بتفرز الخلية ال substrate
+
+73
+00:05:41,150 --> 00:05:45,270
+ممكن إنها تكون جوا الخلية و ممكن إنها تكون برا
+
+74
+00:05:45,270 --> 00:05:49,050
+الخلية هلأ لو كانت ال substrate داخل الخلية مافي
+
+75
+00:05:49,050 --> 00:05:52,730
+عندي مشكلة ال enzyme يفرز من داخل الخلية ال
+
+76
+00:05:52,730 --> 00:05:55,410
+substrate موجودة داخل الخلية التفاعل هيكون
+
+77
+00:05:55,410 --> 00:06:00,980
+intracellularلكن لو كانت الـ substrate خارج الخلية
+
+78
+00:06:00,980 --> 00:06:05,620
+والإنزيم أكيد هيتم إنتاجه داخل الخلية والإنزيم
+
+79
+00:06:05,620 --> 00:06:10,520
+Indoenzyme لازم التفاعل يصير Intracellular عشان
+
+80
+00:06:10,520 --> 00:06:15,820
+هيك الخلية هتفرز إنزيمات هذه الإنزيمات وظيفتها
+
+81
+00:06:15,820 --> 00:06:19,380
+أنها تدخل ال substrate لداخل الخلية عشان يصير
+
+82
+00:06:19,380 --> 00:06:25,410
+التفاعل Intracellularيبقى بالنسبة للـ Endoenzyme
+
+83
+00:06:25,410 --> 00:06:28,590
+الـ enzyme يفرز داخل الخلية ال substrate ممكن تكون
+
+84
+00:06:28,590 --> 00:06:32,230
+داخل الخلية وممكن تكون خارج الخلية إذا كانت داخل
+
+85
+00:06:32,230 --> 00:06:35,570
+الخلية مافي عندى اي مشكلة لو كانت خارج الخلية
+
+86
+00:06:35,570 --> 00:06:39,510
+وقتها الخلية هتفرز انزيمات هذه الانزيمات وضيفتها
+
+87
+00:06:39,510 --> 00:06:42,370
+انها تدخل ال substrate لجوا الخلية عشان يصير
+
+88
+00:06:42,370 --> 00:06:47,570
+التفاعل داخل الخلية من الأمثلة على الـ Endoenzyme
+
+89
+00:06:47,570 --> 00:06:53,810
+أكسيداز كتلاز بيورياز نيترات ريدكتازطبعا هدول حفظ
+
+90
+00:06:53,810 --> 00:06:57,630
+هنتعرف عليهم في المعامل اللي جاي هناخد كل الفحص و
+
+91
+00:06:57,630 --> 00:07:01,930
+نتعرف على ال principle لكل الفحص و كيف بنشتغل هذا
+
+92
+00:07:01,930 --> 00:07:07,870
+الفحص بالنسبة لـ Exoenzyme الـ Exoenzyme مكان حدوث
+
+93
+00:07:07,870 --> 00:07:13,670
+التفاعل خارج الخلية extra cellular where substrate
+
+94
+00:07:13,670 --> 00:07:18,290
+and enzyme react outside the cell بقول ال enzyme و
+
+95
+00:07:18,290 --> 00:07:23,330
+ال substrate هيتفاعلوا خارج الخليةالانزايم برضه
+
+96
+00:07:23,330 --> 00:07:28,170
+هتفرز الخلية هيفرز داخل الخلية لكن ال substrate
+
+97
+00:07:28,170 --> 00:07:32,130
+هتكون فقط خارج الخلية احنا قلنا في ال endoenzyme
+
+98
+00:07:32,130 --> 00:07:36,210
+ممكن تكون داخل وممكن تكون خارج لكن في ال exoenzyme
+
+99
+00:07:36,210 --> 00:07:40,990
+ال substrate هتكون خارج الخليةما بقدر أدخل الـ
+
+100
+00:07:40,990 --> 00:07:43,970
+Substrate زي ما عملت في الـ Endoenzyme لداخل
+
+101
+00:07:43,970 --> 00:07:48,290
+الخلية لأن حجم الـ Substrate كبير عشان كده الـ
+
+102
+00:07:48,290 --> 00:07:53,490
+Enzyme بيطلع لبرا يبقى الـ Exoenzyme الـ Enzyme
+
+103
+00:07:53,490 --> 00:07:57,230
+بيفرز داخل الخلية بيطلع لبرا عند الـ Substrate لأن
+
+104
+00:07:57,230 --> 00:08:01,570
+الـ Substrate حجمها كبير و بيصير التفاعل بين الـ
+
+105
+00:08:01,570 --> 00:08:06,130
+Enzyme والـ Substrate خارج الخلية من الأمثلة على
+
+106
+00:08:06,130 --> 00:08:11,180
+الـ Exoenzyme وحفظهPreco-adiolase جيلاتيناز أميلاز
+
+107
+00:08:11,180 --> 00:08:16,360
+ليباز كازيناز يبقى هدول أمثلة على الـ Exoenzyme
+
+108
+00:08:16,360 --> 00:08:20,240
+يبقى هذا بالنسبة للـ site of reaction قسمنا
+
+109
+00:08:20,240 --> 00:08:25,320
+الإنزيمات لإندوإنزايم and إكزوإنزايم بالنسبة
+
+110
+00:08:25,320 --> 00:08:30,780
+للإنزايم production موقع أو حسب الإنتاج حسب
+
+111
+00:08:30,780 --> 00:08:34,520
+الإنتاج بقسمه لinducible and constitutive
+
+112
+00:08:34,520 --> 00:08:40,300
+inducibleوconstitutive شو الفرق بينهم؟ بقولي الـ
+
+113
+00:08:40,300 --> 00:08:45,120
+inducible بكون عندي محفظ يتم إنتاج ال enzyme فقط
+
+114
+00:08:45,120 --> 00:08:50,260
+في وجود محفظ المحفظ هيحفظ هذا ال enzyme produce
+
+115
+00:08:50,260 --> 00:08:56,760
+only when needed or induced فقط عندما يكون المحفظ
+
+116
+00:08:56,760 --> 00:09:02,040
+يتم إنتاج هذا ال enzyme لكن الconstitutive بيتم
+
+117
+00:09:02,040 --> 00:09:07,590
+إنتاجه بشكل مستمر continuouslyبشكل مستمر بغض النظر
+
+118
+00:09:07,590 --> 00:09:13,350
+فيه عندي محفز أو لأ هذا بالنسبة لأنواع الانزيم حسب
+
+119
+00:09:13,350 --> 00:09:17,050
+الانزيم ال production حسب الإنتاج يبقى type of
+
+120
+00:09:17,050 --> 00:09:20,550
+enzyme حسب ال site of reaction انسو انزيم و اندو
+
+121
+00:09:20,550 --> 00:09:25,610
+انزيم حسب الانزيم ال production ل inducible و
+
+122
+00:09:25,610 --> 00:09:26,690
+constitutive
+
+123
+00:09:36,140 --> 00:09:40,920
+notes ملاحظات مهمة every genus of bacteria has its
+
+124
+00:09:40,920 --> 00:09:44,880
+unique set of enzymes so we can identify it بقول
+
+125
+00:09:44,880 --> 00:09:48,840
+لي كل مجموعة من البكتيريا إلها أنزيمات خاصة فيها
+
+126
+00:09:48,840 --> 00:09:54,320
+هذا بيساعدني أني أنا أعمل identification للبكتيريا
+
+127
+00:09:54,320 --> 00:09:59,300
+على سبيل المثال ال gram positive cocci بتضم
+
+128
+00:09:59,300 --> 00:10:04,460
+لستريبتو cocci و لستافيلو cocciاستفيلوكوكاي عندها
+
+129
+00:10:04,460 --> 00:10:08,720
+انزيم الكتالاز لكن الاستريبتوكوكاي ما عندها انزيم
+
+130
+00:10:08,720 --> 00:10:12,840
+الكتالاز بروح انا بشتغل فحص الكتالاز اذا طلعت
+
+131
+00:10:12,840 --> 00:10:17,240
+كتالاز negative يبقى بحكي انه هي استريبتوكوكاي هي
+
+132
+00:10:17,240 --> 00:10:21,700
+قدرت انا اعمل identification من خلال الانزيمات من
+
+133
+00:10:21,700 --> 00:10:26,980
+خلال وجود انزيمات في بكتيريا معينة وعدم وجودها في
+
+134
+00:10:26,980 --> 00:10:33,090
+بكتيريا اخرىIndoenzyme may act outside the cell in
+
+135
+00:10:33,090 --> 00:10:36,250
+case of the presence of high concentration of its
+
+136
+00:10:36,250 --> 00:10:41,530
+substrate اتفقنا إنه الـIndoenzyme بيفرز داخل
+
+137
+00:10:41,530 --> 00:10:45,690
+الخلية و بيظل داخلها، ما بيطلع برا و بيصير التفاعل
+
+138
+00:10:45,690 --> 00:10:48,510
+داخل الخلية، لو كانت ال substrate برا هي اللي
+
+139
+00:10:48,510 --> 00:10:52,730
+بتدخل لجوة فهذه الجملة بيقول لي في عندي استثناء،
+
+140
+00:10:52,730 --> 00:10:58,330
+احنا قلنا إنه مافي إش عندي absolute أكيد لكل قاعدة
+
+141
+00:10:58,330 --> 00:11:04,580
+شوادممكن الـ Endoenzyme يطلع برا الخلية في حالة
+
+142
+00:11:04,580 --> 00:11:09,720
+واحدة فقط لو كان تركيز الـ substrate خارج الخلية
+
+143
+00:11:09,720 --> 00:11:15,080
+أكتر بكتير من تركيزه في الداخل عشان هيك الـ enzyme
+
+144
+00:11:15,080 --> 00:11:18,880
+هتفرز الخلية ويطلع لبرا عند التركيز العالي من ال
+
+145
+00:11:18,880 --> 00:11:23,980
+substrate ويصير التفاعل هذا بالنسبة للملاحظة
+
+146
+00:11:23,980 --> 00:11:29,840
+التانية الـ Endoenzyme ممكن أنهيطلع خارج الخلية في
+
+147
+00:11:29,840 --> 00:11:33,120
+حال كان عندي الـ Concentration of its substrate
+
+148
+00:11:33,120 --> 00:11:38,440
+تركيزه خارج الخلية أعلى من داخل الخلية آخر ملاحظة
+
+149
+00:11:38,440 --> 00:11:42,840
+The same enzyme could be inducible and constitute
+
+150
+00:11:42,840 --> 00:11:48,320
+in different genera كاتب مثال اليورياسي أحنا قلنا
+
+151
+00:11:48,320 --> 00:11:52,800
+أن الإنزايم يقسم حسب الإنتاج إلى inducible and
+
+152
+00:11:52,800 --> 00:11:59,140
+constitutiveهذه الجملة تقول أن الانزيم نفسه مثلا X
+
+153
+00:11:59,140 --> 00:12:03,640
+ممكن يكون inducible في بكتيريا معينة وconstitute
+
+154
+00:12:03,640 --> 00:12:08,340
+في بكتيريا أخرى من الأمثلة على هذا الانزيم اللي
+
+155
+00:12:08,340 --> 00:12:12,000
+ممكن يكون inducible في بكتيريا وconstitute في
+
+156
+00:12:12,000 --> 00:12:16,960
+بكتيريا أخرى اليوريازي اليورياز من اسمه يورياز
+
+157
+00:12:16,960 --> 00:12:22,230
+هيكسر اليوريااليوريا طبعا هي toxic waste product
+
+158
+00:12:22,230 --> 00:12:27,010
+نتخلص منها عن طريق تكسيرها ل ammonia and CO2
+
+159
+00:12:27,010 --> 00:12:34,910
+بالنسبة لليورياز هيكسر لي اليوريا ل ammonia and
+
+160
+00:12:34,910 --> 00:12:43,190
+CO2 فلو كان بالنسبة لل inducible في بكتيريا اسمها
+
+161
+00:12:43,190 --> 00:12:48,370
+Brutias بتفرز أنزيم اليورياز في موجود محفظ يعني
+
+162
+00:12:49,580 --> 00:12:53,320
+بنعتبرها inducible لما نعتبر الإنزايم HAN
+
+163
+00:12:53,320 --> 00:12:58,080
+inducible لكن في حال عدم وجود المحفظ لا يتم إنتاجه
+
+164
+00:12:58,080 --> 00:13:02,360
+يبقى HAN كان الإنزيم اليوريز inducible في
+
+165
+00:13:02,360 --> 00:13:07,740
+البروتياس فقط في وجود محفظ هيتم إنتاجه لكن فيه
+
+166
+00:13:07,740 --> 00:13:12,400
+اندباتكيريا الهيليكوباكتر بيلوري يتم إنتاجه بشكل
+
+167
+00:13:12,400 --> 00:13:18,610
+مستمر يعني يعتبر constitutiveالـ H-Biolary بتكون
+
+168
+00:13:18,610 --> 00:13:23,030
+في المعدة طريقة الـ infection تقليل حموضة المعدة
+
+169
+00:13:23,030 --> 00:13:28,450
+كيف؟ بتظهر تفرز إنزايم اليورياز عشان تتكسر اليوريا
+
+170
+00:13:28,450 --> 00:13:33,210
+وتنتج الأمانيا الأمانيا طبعا قاعدة هيرتفع الـ pH
+
+171
+00:13:33,210 --> 00:13:38,490
+وتتقلل من حموضة المعدة يبقى الإنزايم نفسه اليورياز
+
+172
+00:13:38,490 --> 00:13:43,390
+ممكن يكون inducible وممكن يكون constitutive ذكرنا
+
+173
+00:13:43,390 --> 00:13:49,400
+مثل اليورياز إنزايمكان Inducable في الـ Brutias
+
+174
+00:13:49,400 --> 00:13:54,540
+فقط في موجود محفظ هيتم إنتاجه وكان Constitutive في
+
+175
+00:13:54,540 --> 00:13:59,600
+الـ H-Biliary في الـ الـ H-Biliary الـ
+
+176
+00:13:59,600 --> 00:14:02,960
+Helicobacter Biliary كان Constitutive هيتم إنتاجه
+
+177
+00:14:02,960 --> 00:14:06,280
+بشكل مستمر لأن طريقة الـ Infection اللي هو تقليل
+
+178
+00:14:06,280 --> 00:14:11,280
+من حموضة المعدة لازم يتم إنتاج Ammonia أو مركب
+
+179
+00:14:11,280 --> 00:14:15,950
+قاعدي هيرتفع الـ pH وتتقلل حموضة المعدةهذا بالنسبة
+
+180
+00:14:15,950 --> 00:14:23,110
+لآخر ملاحظة kinds
+
+181
+00:14:23,110 --> 00:14:27,010
+of bacterial enzymatic reaction أنواع الـ
+
+182
+00:14:27,010 --> 00:14:30,590
+enzymatic reaction أو ليش البكتيريا بتفرز ال
+
+183
+00:14:30,590 --> 00:14:37,010
+enzyme أول نوع the breakdown of toxic wastesuch as
+
+184
+00:14:37,010 --> 00:14:41,390
+a hydrogen peroxide or urea بقول ليه بتتخلص منه ال
+
+185
+00:14:41,390 --> 00:14:46,170
+worst product زي ال urea and hydrogen peroxide ال
+
+186
+00:14:46,170 --> 00:14:52,270
+H2O2 طبعا هدول ال H2O2 و ال urea يعتبروا toxic
+
+187
+00:14:52,270 --> 00:14:57,110
+طبعا بيتم يهم فضلات بيتم إنتاجهم من عمليات عيضية
+
+188
+00:14:57,110 --> 00:15:01,430
+عشان أتخلص من هذه الفضلات بيتم إفراز أنزيم هذه
+
+189
+00:15:01,430 --> 00:15:06,530
+الانزيمالانزيمات بتتخلص من هذه الـ toxic material
+
+190
+00:15:06,530 --> 00:15:12,190
+عشان أتخلص من اليوريا بتفرز البكتيريا انزيم
+
+191
+00:15:12,190 --> 00:15:16,810
+اليورياز عشان أتخلص من الـ hydrogen peroxide الـ
+
+192
+00:15:16,810 --> 00:15:23,570
+H2O2 بتفرز البكتيريا انزيم الكتلازي هذا بالنسبة
+
+193
+00:15:23,570 --> 00:15:27,610
+لأول نوع من ال enzymatic reaction اللي هو أني أنا
+
+194
+00:15:27,610 --> 00:15:31,300
+أتخلص من الـ toxic materialالتاني نوع The
+
+195
+00:15:31,300 --> 00:15:37,100
+reduction of Nitrate or Oxygen اختزال للنيترات او
+
+196
+00:15:37,100 --> 00:15:40,900
+الأكسجين مثال عليها الـ Nitrate Reductase من اسمه
+
+197
+00:15:40,900 --> 00:15:44,840
+Nitrate Reductase هي عمل Reduction للنيترات
+
+198
+00:15:44,840 --> 00:15:49,940
+بالنهاية عشان احصل على ال nitrogen source اللازم
+
+199
+00:15:49,940 --> 00:15:54,730
+لنمو البكتيرياتالت نوع الـ degradation of specific
+
+200
+00:15:54,730 --> 00:15:59,530
+amino acid بيكسّل لبعض الأحماض الأمينية زي الـ
+
+201
+00:15:59,530 --> 00:16:03,630
+tryptophan الـ tryptophan amino acid بيتم تكسيره
+
+202
+00:16:03,630 --> 00:16:07,810
+بواسطة أنزيم الـ tryptophanase تمام، عشان أحصل
+
+203
+00:16:07,810 --> 00:16:12,370
+بالنهاية على carbon and nitrogen source لازم لـ
+
+204
+00:16:12,370 --> 00:16:17,390
+growth، لنموء البكتيريا رابع نوع الـ utilization
+
+205
+00:16:17,390 --> 00:16:22,040
+of non-carbohydrate carbon source for growthfor
+
+206
+00:16:22,040 --> 00:16:27,400
+example اليورياز بقوللي استهلاك non carbohydrate
+
+207
+00:16:27,400 --> 00:16:32,840
+source اللازم لنمو البكتيريا زي اليورياز حتى أحصل
+
+208
+00:16:32,840 --> 00:16:37,480
+على nitrogen and carbon source عشان تنمو البكتيريا
+
+209
+00:16:37,480 --> 00:16:41,760
+بحتاج ل carbon source و nitrogen source ال carbon
+
+210
+00:16:41,760 --> 00:16:45,220
+source ممكن تكون مصدرها ال carbohydrate أو المصدر
+
+211
+00:16:45,220 --> 00:16:48,590
+الأول لإلها ال carbohydrateفي حال الـ Carbohydrate
+
+212
+00:16:48,590 --> 00:16:53,010
+خلصت بروح لمصادر أخرى اللي هي الـ Non-Carbohydrate
+
+213
+00:16:53,010 --> 00:16:56,230
+Carbon Source من الأمثلة على الـ Non-Carbohydrate
+
+214
+00:16:56,230 --> 00:17:01,730
+Carbon Source زي اليوريا السيطرات عشان أستهلك
+
+215
+00:17:01,730 --> 00:17:06,690
+اليوريا والسيطرات أكيد في عندي انزيمات من الأمثلة
+
+216
+00:17:06,690 --> 00:17:10,550
+على الانزيمات اليورياز اليورياز هيكسرلي اليوريا
+
+217
+00:17:10,550 --> 00:17:14,650
+وأقدر ان احصل انا على Carbon Source كذلك السيطرات
+
+218
+00:17:15,360 --> 00:17:20,920
+كمان يُعتبر مثال على الـ Utilization of non
+
+219
+00:17:20,920 --> 00:17:24,740
+-carbohydrate carbon source هو مصدر للكربون لكن
+
+220
+00:17:24,740 --> 00:17:29,160
+non-carbohydrate مش من الكربوهيدرات مثال عليه الـ
+
+221
+00:17:29,160 --> 00:17:34,200
+Ureas هذا النوع و الـ citrate هذا بالنسبة للـ
+
+222
+00:17:34,200 --> 00:17:37,500
+bacterial enzymatic reaction إذا ملاحظ الـ Ureas
+
+223
+00:17:37,500 --> 00:17:44,240
+يُعتبر إله نوعين أو إله هدفين إن أنا أتخلص من الـ
+
+224
+00:17:44,240 --> 00:17:48,610
+toxicWest اللي هي اليوريا و بنفس الوقت بدي أحصل
+
+225
+00:17:48,610 --> 00:17:53,050
+على non carbohydrate carbon source يبقى اليورياز
+
+226
+00:17:53,050 --> 00:17:58,310
+كان في النوع الأول و النوع الرابع خلصنا ال
+
+227
+00:17:58,310 --> 00:18:04,410
+enzymatic reaction هلأ هنبدأ في أول نوع أو أول
+
+228
+00:18:04,410 --> 00:18:10,810
+enzyme اللي هو الكتلازب اسم ال enzyme طبعا من اسم
+
+229
+00:18:10,810 --> 00:18:15,290
+الفحص اللي هو الكتلازبالـ substrate اللي هيشتغل
+
+230
+00:18:15,290 --> 00:18:19,790
+عليها الـ enzyme الـ hydrogen peroxide الـ H2O2
+
+231
+00:18:19,790 --> 00:18:25,270
+اللي قولنا هي toxic material الـ enzyme action
+
+232
+00:18:25,270 --> 00:18:30,850
+break down the toxic H2O2 producing oxygen, gas
+
+233
+00:18:30,850 --> 00:18:36,230
+and water بيقوللي هيكسر ال toxic H2O2 قولنا ان الـ
+
+234
+00:18:36,230 --> 00:18:40,530
+H2O2 الـ hydrogen peroxide يعتبر toxic waste
+
+235
+00:18:40,530 --> 00:18:46,150
+productهيتكسر لـ O2 Gas and Water المعادلة مضحة
+
+236
+00:18:46,150 --> 00:18:50,050
+تفاعل الـ H2O2 قلنا الـ Substrate الإنزايم الـ
+
+237
+00:18:50,050 --> 00:18:54,650
+Catalase نواتج التفاعل O2 الـ Gas and Water الـ
+
+238
+00:18:54,650 --> 00:19:00,550
+H2O2 قلنا من أنواع الـ enzymatic reaction إنه يكسر
+
+239
+00:19:00,550 --> 00:19:05,110
+لي ال worst product الناتج من العمليات الأيضية ال
+
+240
+00:19:05,110 --> 00:19:10,830
+Catalase هيكسر الـ H2O2 الناتج من عمليةالتنفس
+
+241
+00:19:10,830 --> 00:19:17,090
+الخلوي الـ aerobic respiration يبقى الـ H2O2 هيتم
+
+242
+00:19:17,090 --> 00:19:20,750
+إنتاجه خلال عملية التنفس الخلوي الـ aerobic
+
+243
+00:19:20,750 --> 00:19:26,630
+respiration كيف بيتم إنتاج الـ H2O2 في عملية
+
+244
+00:19:26,630 --> 00:19:32,010
+التنفس الخلوي في انديانة electron transport chain
+
+245
+00:19:38,180 --> 00:19:41,240
+في عندي انات، electron
+
+246
+00:20:08,540 --> 00:20:13,180
+في عندنا electron transport chain بيتم التقال
+
+247
+00:20:13,180 --> 00:20:17,340
+الإلكترون من جزء لجزء حتى يوصل للمستقبل النهائي
+
+248
+00:20:17,340 --> 00:20:21,420
+للإلكترونات المستقبل النهائي للإلكترونات هو
+
+249
+00:20:21,420 --> 00:20:27,680
+الأكسجين هيرتبط الإلكترون بالمداري الأخر للأكسجين
+
+250
+00:20:27,680 --> 00:20:30,260
+حسب المعادلة التالية
+
+251
+00:20:41,340 --> 00:20:55,740
+طبعا هذا ال Oxygen يبقى
+
+252
+00:20:55,740 --> 00:20:59,280
+هيرتبط ال Electron خلال انتقاله في ال Electronic
+
+253
+00:20:59,280 --> 00:21:03,460
+Transport لكي ينتقل بيه للمستقبل أو هيرتبط بيه
+
+254
+00:21:03,460 --> 00:21:07,120
+المستقبل النهائي للإلكترونات اللي هو ال Oxygen هو
+
+255
+00:21:07,120 --> 00:21:09,840
+يعطيني ال Super Oxide
+
+256
+00:21:29,030 --> 00:21:38,570
+هيعطيني ال super oxide هذا بسميه ال
+
+257
+00:21:38,570 --> 00:21:47,030
+super
+
+258
+00:21:47,030 --> 00:21:58,170
+oxide او ال free radical ممكن نسميه هذا
+
+259
+00:21:58,170 --> 00:22:01,870
+ال superالـ Oxide أو الـ Free Radical يعتبر طبعا
+
+260
+00:22:01,870 --> 00:22:07,150
+Toxic Unstable طبعا هنتخلص منه في عندي في ال
+
+261
+00:22:07,150 --> 00:22:12,070
+Electronic Transport اتشان في عندي مضخات لـ H Plus
+
+262
+00:22:12,070 --> 00:22:16,550
+تمام الـ H Plus فبالتالي الـ Super Oxide هذا
+
+263
+00:22:16,550 --> 00:22:22,270
+هيرتبط بالـ H Plus ويعطيني الـ Hydrogen Peroxide
+
+264
+00:22:22,270 --> 00:22:23,850
+نكمل المعادلة
+
+265
+00:22:33,320 --> 00:22:37,780
+ال super oxide هذا unstable free radical toxic
+
+266
+00:22:37,780 --> 00:22:42,040
+يعتبر عشان اتخلص منه خلال او موجود فيه ال
+
+267
+00:22:42,040 --> 00:22:51,540
+electronic transport اتشان في عندي ال H plus ال
+
+268
+00:22:51,540 --> 00:22:56,260
+H plus بواسطة انزيم ال super oxide ديسميوتاز
+
+269
+00:22:56,260 --> 00:23:00,980
+هيتحول ل ال H2O2 اللي هو ال hydrogen peroxide
+
+270
+00:23:39,680 --> 00:23:45,400
+وسط انزيم السوبر اوكسايد دي سيميوتاز هيتحول لـ H2O
+
+271
+00:23:45,400 --> 00:23:54,740
+اللي
+
+272
+00:23:54,740 --> 00:24:00,020
+هو الهيدروجين بيروكسايد طبعا
+
+273
+00:24:00,020 --> 00:24:03,680
+ممكن هنوزن المعادلة اللي هي هاندفع اتنين
+
+274
+00:24:11,990 --> 00:24:17,130
+هكذا هذا هو الهيدروجين بيروكسايد هيك اتكون
+
+275
+00:24:17,130 --> 00:24:22,870
+الهيدروجين بيروكسايد هيتكسر لجاز and water بواسطة
+
+276
+00:24:22,870 --> 00:24:25,970
+انزيم الكتالاز هنتخلص الهيدروجين بيروكسايد يعتبر
+
+277
+00:24:25,970 --> 00:24:31,050
+toxic ل ال DNA و ل ال cell membrane عشان نتخلص منه
+
+278
+00:24:31,050 --> 00:24:35,850
+هيتم افراز انزيم الكتالاز الكتالاز هيتكسر ليا ل O2
+
+279
+00:24:35,850 --> 00:24:41,910
+and H2O لwater يبقىفي الـ Electron Transport Chain
+
+280
+00:24:41,910 --> 00:24:46,690
+في عندي الإلكترونات هتنتقل من جزء للجزء حتى تصل
+
+281
+00:24:46,690 --> 00:24:50,210
+للمستقبل إنها اللي هو الـ Oxygen هترتبط بالـ
+
+282
+00:24:50,210 --> 00:24:54,050
+Oxygen في المداري الأخير هتعطيني الـ Super Oxide
+
+283
+00:24:54,050 --> 00:25:00,250
+لـ Free Radical الـ R2-هذا الـ Free Radical
+
+284
+00:25:00,250 --> 00:25:05,430
+Unstable هيرتبط بالـ H Plus هاي الـ H Plus موجود
+
+285
+00:25:05,430 --> 00:25:08,550
+طبعا فيها اندي مضخات في الـ Electron Transported
+
+286
+00:25:08,550 --> 00:25:11,510
+Channel اللي هترتبط فيها بواسطة انزيم الـ Super
+
+287
+00:25:11,510 --> 00:25:16,070
+Oxide Dismutase هيعطيني الـ Hydrogen Peroxide هذا
+
+288
+00:25:16,070 --> 00:25:21,030
+بالنسبة لكيف تم إنتاج الـ H2O2 من عملية التنفس
+
+289
+00:25:21,030 --> 00:25:21,870
+الخلوي
+
+290
+00:25:25,820 --> 00:25:30,580
+بقول لي إن الـ hydrogen peroxide طبعا لازم يتكسر
+
+291
+00:25:30,580 --> 00:25:35,240
+to have to be breakdown to prevent its toxic
+
+292
+00:25:35,240 --> 00:25:39,580
+action عشان أمنع ال toxic action on DNA and cell
+
+293
+00:25:39,580 --> 00:25:44,200
+membrane قولنا هو toxic لل DNA and cell membrane
+
+294
+00:25:51,590 --> 00:25:55,170
+طيب كيف بدي أكشف هل البكتيريا عندها انزايم
+
+295
+00:25:55,170 --> 00:25:59,930
+الكتالاز او لأ قلنا بنجيب مادة أحكي فيها ال
+
+296
+00:25:59,930 --> 00:26:03,290
+substrate اللي بيشتغل عليها ال enzyme احنا قلنا ان
+
+297
+00:26:03,290 --> 00:26:07,970
+ال substrate اللي بيشتغل عليها الكتالاز هي ال H2O2
+
+298
+00:26:07,970 --> 00:26:11,910
+ال hydrogen peroxide بنجيب مادة كيميائية من
+
+299
+00:26:11,910 --> 00:26:18,510
+المختور اللي هي ال hydrogen peroxide 3% H2O2 بنضيف
+
+300
+00:26:18,510 --> 00:26:23,390
+عليه كولونيهذه الكولونية طبعاً إذا كانت الكتلاز
+
+301
+00:26:23,390 --> 00:26:28,170
+البكتيريا موجود فيها انزيم الكتلاز هيتكسر بواسطة
+
+302
+00:26:28,170 --> 00:26:33,090
+الانزيم وينتج Oxygen ال Oxygen بشوفه على شكل air
+
+303
+00:26:33,090 --> 00:26:36,590
+bubble يبقى when hydrogen peroxide is added to a
+
+304
+00:26:36,590 --> 00:26:39,630
+colony of كتلاز producing بكتيريا لو أنا ضفت ال
+
+305
+00:26:39,630 --> 00:26:44,820
+hydrogen peroxideالـ hydrogen peroxide اللي هو الـ
+
+306
+00:26:44,820 --> 00:26:49,700
+substrate 3% H2O توضفته للكولونيه للبكتيريا اللي
+
+307
+00:26:49,700 --> 00:26:54,580
+بتفرز الكتاليز أكيد هيتكسر لأكسجين، الأكسجين بقدر
+
+308
+00:26:54,580 --> 00:26:57,580
+أني أنا أكشف عنه من خلال أني أنا بشوفه على شكل
+
+309
+00:26:57,580 --> 00:27:02,320
+bubbles significance، significance هتمر معناه
+
+310
+00:27:02,320 --> 00:27:05,920
+كتير، كل فحص كيميائي لازم يكون فيه له significance
+
+311
+00:27:05,920 --> 00:27:10,190
+أو لازم نعرف شو ال significance لهذا الفحصالـ
+
+312
+00:27:10,190 --> 00:27:14,170
+Significance يعني شو أهمية الفحص بيفرق بين مين
+
+313
+00:27:14,170 --> 00:27:18,050
+ومين مين الـ Positive و الـ Negative لهذا الفحص
+
+314
+00:27:18,050 --> 00:27:23,930
+قبل من نتكلم على هاي ال slide بدنا نرسم نبدأ من
+
+315
+00:27:23,930 --> 00:27:33,790
+الـ Gram Stain لو أنا عملت نرسم Diagram عملت
+
+316
+00:27:33,790 --> 00:27:34,970
+Gram Stain
+
+317
+00:27:53,530 --> 00:27:56,590
+قلنا لـ Gram Stain بتقسم البكتيريا لـ Gram
+
+318
+00:27:56,590 --> 00:27:58,070
+Positive و Gram Negative
+
+319
+00:28:35,260 --> 00:28:39,080
+يبقى لجرام 2 تقسم للبكتيريا لجرام positive و لجرام
+
+320
+00:28:39,080 --> 00:28:42,380
+negative كمان لجرام positive و لجرام negative
+
+321
+00:28:42,380 --> 00:28:45,980
+تتقسم لإنها يصابغة لككاي و بصلاي
+
+322
+00:29:24,040 --> 00:29:41,880
+Okay بس ال line بس
+
+323
+00:29:41,880 --> 00:29:42,260
+ال line
+
+324
+00:29:54,170 --> 00:29:57,930
+يبقى بالنهاية قسمتلي إلى gram positive كوكاي gram
+
+325
+00:29:57,930 --> 00:30:01,350
+positive بصل لاي gram negative كوكاي gram negative
+
+326
+00:30:01,350 --> 00:30:06,470
+بصل لاي قلنا ان كل مجموع بتضم مثلا ل gram positive
+
+327
+00:30:06,470 --> 00:30:10,130
+كوكاي بتضم مجموعة من البكتيريا ل gram positive بصل
+
+328
+00:30:10,130 --> 00:30:13,570
+لاي بتضم مجموعة من البكتيريا كيف بدي افرق بينهم
+
+329
+00:30:13,570 --> 00:30:17,980
+باستخدام ال biochemical testالجرام بوزيتف بكتيريا
+
+330
+00:30:17,980 --> 00:30:21,980
+بتضم الاستريبتو كوكاي الاستافيلو كوكاي الماكرو
+
+331
+00:30:21,980 --> 00:30:28,020
+كوكاي والمايكرو كوكاي بقوللي عشان افرق بين هدال
+
+332
+00:30:28,020 --> 00:30:33,980
+المجموعة بعمل فحص الكتلاز طبعا يفقه لو طلعت عملت
+
+333
+00:30:33,980 --> 00:30:37,280
+جرامستان وطلعت معايا جرام بوزيتف كوكاي على طول
+
+334
+00:30:37,280 --> 00:30:43,150
+بعمل فحص الكتلازعشان افرق بين الستريبتو كوكاي
+
+335
+00:30:43,150 --> 00:30:46,790
+وباقي الأنواع الاستفيلو والماكرو والمايكرو يبقى
+
+336
+00:30:46,790 --> 00:30:49,890
+الخطوة التانية اني انا بدي اعمل ال catalase test
+
+337
+00:31:24,380 --> 00:31:39,100
+الـ Catalase Test لو
+
+338
+00:31:39,100 --> 00:31:41,780
+عملت ال Catalase Test وطلع Negative
+
+339
+00:31:57,390 --> 00:32:00,230
+يبقى البكتيريا هي Striptococcal
+
+340
+00:32:45,870 --> 00:32:49,830
+هذا بالنسبة لكتلازن ال test لو طلعت عملت الفحص و
+
+341
+00:32:49,830 --> 00:32:53,910
+طلعت negative يبقى هي streptococci لكن لو طلعت
+
+342
+00:32:53,910 --> 00:33:03,410
+positive في عندي تلت احتمالات اما Staphylococci
+
+343
+00:33:03,410 --> 00:33:13,670
+Staphylococci او macrococci او micrococci او
+
+344
+00:33:13,670 --> 00:33:14,270
+micro
+
+345
+00:33:28,760 --> 00:33:34,220
+Micrococci Staphylococci Macrococci
+
+346
+00:33:34,220 --> 00:33:38,000
+Micrococci يبقى هي positive يبقى فحص الكتلاز فرق
+
+347
+00:33:38,000 --> 00:33:41,060
+اللي بين الاستربتو cocci وما بين الاستفيلو
+
+348
+00:33:41,060 --> 00:33:46,130
+والماكرو والمايكرو cocciالـ Gram Positive Bacilli
+
+349
+00:33:46,130 --> 00:33:52,390
+برضه بتضم البصلص والكلستريديوم البصلص لو انا عملت
+
+350
+00:33:52,390 --> 00:33:57,270
+فحص الكتلاز البصلص هو positive والكلستريديوم
+
+351
+00:33:57,270 --> 00:34:01,570
+negative تمام وفي عندي كمان ال Gram Negative
+
+352
+00:34:01,570 --> 00:34:08,950
+Bacilli بتضم الاستيريا مونو سايتو جين يبقى ال Gram
+
+353
+00:34:08,950 --> 00:34:12,790
+Positive Bacilli في عندي أنا بعمل فحص الكتلاز نفس
+
+354
+00:34:12,790 --> 00:34:13,310
+الفكرة
+
+355
+00:34:43,130 --> 00:34:46,030
+يبقى الـ gram positive بصل لاي بتضم البصلص
+
+356
+00:34:46,030 --> 00:34:50,230
+Clostridium والاستيريا مونو سايتوجين عشان افرق
+
+357
+00:34:50,230 --> 00:34:55,110
+بينهم بعمل فحص الكتلاز لو طلعت كتلاز positive يبقى
+
+358
+00:34:55,110 --> 00:34:58,530
+في عندي احتمال اما انها تكون بصلص او الاستيريا
+
+359
+00:34:58,530 --> 00:35:03,010
+مونو سايتوجين لو كانت negative يبقى هي Clostridium
+
+360
+00:35:03,010 --> 00:35:07,350
+يبقى هي بتكون بصلص عندي او Clostridium او
+
+361
+00:35:07,350 --> 00:35:11,280
+الاستيريا مونو سايتوجين بعمل فحص الكتلازلو طلعت
+
+362
+00:35:11,280 --> 00:35:28,860
+positive أو طلعت negative لو كانت negative لو
+
+363
+00:35:28,860 --> 00:35:31,620
+كانت negative يبقى هي a closed medium
+
+364
+00:36:06,780 --> 00:36:12,780
+لكن لو كانت positive فى عندى احتمالين اما
+
+365
+00:36:12,780 --> 00:36:19,400
+انها تكون bustle loss او
+
+366
+00:36:19,400 --> 00:36:25,440
+انها تكون listeria monocytogen bustle
+
+367
+00:36:25,440 --> 00:36:30,580
+loss or listeria
+
+368
+00:36:30,580 --> 00:36:35,600
+mono
+
+369
+00:36:44,510 --> 00:36:51,410
+Cytogen يبقى البصلص والستيريا مونو Cytogen كتلاز
+
+370
+00:36:51,410 --> 00:36:59,530
+positive كيف
+
+371
+00:36:59,530 --> 00:37:03,250
+نفرق بين الاستفيلو والماكرو والمايكرو هنتعرف عليها
+
+372
+00:37:03,250 --> 00:37:07,410
+في المعامل الجاي لكن بالنسبة للبصلص والستيريا مونو
+
+373
+00:37:07,410 --> 00:37:11,420
+Cytogenلو انا بدي افرق بينهم بفحص اللي هو الـ
+
+374
+00:37:11,420 --> 00:37:32,820
+Sparkstan Sparkstan Sparkstan
+
+375
+00:37:34,310 --> 00:37:38,230
+بعمله لو طلعت أسبار farming يبقى هي البصي اللاص
+
+376
+00:37:38,230 --> 00:37:42,390
+لكن لو طلعت من أسبار farming يبقى هي الاستيريا
+
+377
+00:37:42,390 --> 00:37:46,750
+مونو سايتوجين هك انا اقدرت ابدأ اتعرف على
+
+378
+00:37:46,750 --> 00:37:49,870
+البكتيريا يبقى لو عملت gram stand طلعت gram
+
+379
+00:37:49,870 --> 00:37:53,840
+positiveفي عندى احتمال استريبتو كوكاي استفيلو
+
+380
+00:37:53,840 --> 00:37:56,780
+كوكاى الماكرو كوكاى المايكرو كوكاى كيف بدي افرق
+
+381
+00:37:56,780 --> 00:37:59,700
+بينهم بعمل فحص ال catalase لو طلعت negative يبقى
+
+382
+00:37:59,700 --> 00:38:02,880
+هى استريبتو كوكاى لو طلعت positive فى عندى تلت
+
+383
+00:38:02,880 --> 00:38:06,260
+احتمالات كيف افرق بينهم وان نتعرف في المعامل اللى
+
+384
+00:38:06,260 --> 00:38:10,640
+جاي فى الفحوصات اللى هتمر معناهالو عملت جرامستين و
+
+385
+00:38:10,640 --> 00:38:14,020
+طلعت جرام positive بصل لاي في عندي الجرام positive
+
+386
+00:38:14,020 --> 00:38:17,380
+بصل لاي بتضم لكلوستريديوم البصلص الستيريا مونو
+
+387
+00:38:17,380 --> 00:38:20,900
+سايتوجين كاف دي افرق بينهم وبعمل فحص الكتلاز لو
+
+388
+00:38:20,900 --> 00:38:24,140
+طلعت نيجاتيف يبقى هي الكلوستريديوم لو طلعت
+
+389
+00:38:24,140 --> 00:38:27,980
+positive يبقى عندي احتمالان اما البصلص او الستيريا
+
+390
+00:38:27,980 --> 00:38:31,040
+مونو سايتوجين كاف دي افرق بينهم هذه المعلومة
+
+391
+00:38:31,040 --> 00:38:35,560
+أخدناها في المعامل السابقة من خلال الالباصيلاز
+
+392
+00:38:35,560 --> 00:38:39,000
+تعتبر Spar Farming لكن الاستيريا مونو سايتوجين
+
+393
+00:38:39,000 --> 00:38:44,840
+تعتبر Non Spar Farming لجرام نيجاتف كوككاي في عندي
+
+394
+00:38:44,840 --> 00:38:48,020
+النايسيريا والموريكز اللي هنتعرف عليهم برضه في
+
+395
+00:38:48,020 --> 00:38:51,160
+المعامل اللي جاي لجرام نيجاتف باصيلاي في عند ال
+
+396
+00:38:51,160 --> 00:38:55,500
+انتيرو بكترياسي و الصودوموناص هدول برضه كتلازة
+
+397
+00:38:55,500 --> 00:38:57,880
+تنتان كتلازة positive بيبقى هذا الفحص أنا
+
+398
+00:38:57,880 --> 00:39:02,220
+مابستخدمه لا تفريقهناخد فحوصات أقدر أنا أميز بين
+
+399
+00:39:02,220 --> 00:39:03,860
+الـ Gram Negative بس الـ Lie اللي هي الـ
+
+400
+00:39:03,860 --> 00:39:07,400
+Enterobacteriasi و الـ Sodomonas في المعامل الجاي
+
+401
+00:39:07,400 --> 00:39:13,320
+هيك خلصنا احنا الـ Significant لهذا الفحص هنمر على
+
+402
+00:39:13,320 --> 00:39:13,920
+الـ Slides
+
+403
+00:39:31,100 --> 00:39:34,900
+الـ Significant لهذا الفحص هيفرق لبن لـ Gram
+
+404
+00:39:34,900 --> 00:39:38,360
+Positive كوكاية قلنا لستفيلو كوكاية هي كتلاز
+
+405
+00:39:38,360 --> 00:39:42,460
+positive لكن لستربتو كوكاية كتلاز negative كمان
+
+406
+00:39:42,460 --> 00:39:46,540
+هيفرق ل Gram Positive بصلاي قلنا الباصلة is كتلاز
+
+407
+00:39:46,540 --> 00:39:50,400
+positive where Clostridium is كتلاز negative ال
+
+408
+00:39:50,400 --> 00:39:54,480
+Antirobacterias اللي هي تعتبر Gram Negative بصلاي
+
+409
+00:39:54,760 --> 00:39:57,720
+ار كتلاز بوزيتف قلنا لجرام نيجاتف بس الله عند
+
+410
+00:39:57,720 --> 00:40:01,380
+الانتيرو بكترياس يوصو ضموناس والدين تاني يعتبروا
+
+411
+00:40:01,380 --> 00:40:05,820
+كتلاز بوزيتف كيف هنفرق بينهم في عند فحص الأكسداز
+
+412
+00:40:05,820 --> 00:40:11,280
+هيمور معنا في الشباتر الجايالـ Stereomonocytogen
+
+413
+00:40:11,280 --> 00:40:14,740
+قلنا هي gram positive بصلاي وهي كتلاز positive كيف
+
+414
+00:40:14,740 --> 00:40:20,040
+بدي افرق بينها وبين البصلص قلنا في عين دي فحص اللي
+
+415
+00:40:20,040 --> 00:40:23,840
+هو فحص الـ spar stand هي non spar farming لكن
+
+416
+00:40:23,840 --> 00:40:30,480
+البصلص spar farming الفحص بيفرق بين ال .. أي فحص
+
+417
+00:40:30,480 --> 00:40:34,540
+هيفرقلي بين اللي بيكونوا بنفس المجموعةيعني ما بقدر
+
+418
+00:40:34,540 --> 00:40:38,720
+أحكي عن الـ Catalase يستخدم للتفريق بين الـ Closed
+
+419
+00:40:38,720 --> 00:40:41,700
+Medium و الـ Staff لأن الـ Closed Medium بالاصل هي
+
+420
+00:40:41,700 --> 00:40:44,880
+إجرام Positive بص الله و الـ Staff إجرام Positive
+
+421
+00:40:44,880 --> 00:40:49,980
+كوكاية يعني بالاصل أنا بقدر أني أنا أفصل بينهم
+
+422
+00:40:49,980 --> 00:40:54,540
+الفحص نفسه هو بيفرق بين اللي بيكونوا بنفس المجموعة
+
+423
+00:40:54,540 --> 00:40:57,880
+يعني لو كانوا كلهم إجرام Positive كوكاية أو إجرام
+
+424
+00:40:57,880 --> 00:41:02,760
+Positive بص الله و هكذاهيك خلصنا الـ Significant
+
+425
+00:41:02,760 --> 00:41:05,760
+للفحص كيف طريقة الشغل
+
+426
+00:41:09,860 --> 00:41:14,480
+How to do the test فى عندي طريقتين عشان أشتغل هذا
+
+427
+00:41:14,480 --> 00:41:18,100
+الفحص الطريقة الأولى اللى هى slide method فى عندي
+
+428
+00:41:18,100 --> 00:41:21,740
+طريقة تانية لتيوب method ال slide method بقولى
+
+429
+00:41:21,740 --> 00:41:27,380
+بجيب أنا slide بحط drop of 3% hydrogen peroxide
+
+430
+00:41:27,380 --> 00:41:31,840
+اللى هو ال substrate ال H2O2 كيف بدأ أحضر 3%
+
+431
+00:41:31,840 --> 00:41:35,700
+hydrogen peroxide قولنا بنضيف 3 ml من ال H2O2
+
+432
+00:41:35,700 --> 00:41:41,100
+موجود كيميائى هو محضر كيميائىبنضيف عليه ديستيل
+
+433
+00:41:41,100 --> 00:41:48,360
+واتر 97 مل من ديستيل واتر بنضيف هذا الرياجينت على
+
+434
+00:41:48,360 --> 00:41:52,920
+جلوس صافية بعدين باخد Loop of test organism باخد
+
+435
+00:41:52,920 --> 00:41:56,780
+كولونيه لبكتيريا اللى انا بدي اكشف هل موجود
+
+436
+00:41:56,780 --> 00:42:02,300
+الانزيم عندها او لأ بعمل mix بعدين بلاحظ النتيجة
+
+437
+00:42:03,000 --> 00:42:06,560
+الـ tube method طريقة الـ tube بقولّي أنه أنا بجيب
+
+438
+00:42:06,560 --> 00:42:11,020
+بزرع البكتيريا اللي بدي أكشف عنها بجراء بأجار
+
+439
+00:42:11,020 --> 00:42:21,800
+إسلانت يبقى بدي أزرع بأجار إسلانت بجيب
+
+440
+00:42:21,800 --> 00:42:26,920
+طبعا media nutrient أجار مزروعة بالإسلانت بتكون
+
+441
+00:42:26,920 --> 00:42:30,700
+إسلانت بشكل مائل كيف بنزرع الأجار الإسلانت قلنا
+
+442
+00:42:30,700 --> 00:42:34,690
+بندخلبالجزء الأسفل بنعمل طعنة وبعدين بنطلع بنعمل
+
+443
+00:42:34,690 --> 00:42:38,110
+زيكزاك بعد بأزرع البكتيريا اللى بدي أكشف عنها
+
+444
+00:42:38,110 --> 00:42:42,570
+بعملها incubation في ال incubator لمدة 24 ساعة بعد
+
+445
+00:42:42,570 --> 00:42:47,690
+24 ساعة بلاحظ نمو البكتيريا بقوللي بتضيفي 1 مل من
+
+446
+00:42:47,690 --> 00:42:52,390
+ال reagent اللى هو 3% hydrogen peroxide على اللى
+
+447
+00:42:52,390 --> 00:42:57,610
+هو على سطح الإسلان تمام بعد هبشوف النتيجة كيف ال
+
+448
+00:42:57,610 --> 00:43:00,950
+result بتكون ال result هنشوفها في ال slide اللى
+
+449
+00:43:00,950 --> 00:43:07,270
+بعدالنتيجة لو كانت النتيجة positive هذا بالنسبة لل
+
+450
+00:43:07,270 --> 00:43:11,890
+slide method سيشوف النتيجة ال positive هتكون على
+
+451
+00:43:11,890 --> 00:43:15,570
+شكل air bubble يبقى لو اتكون عندي air bubble يبقى
+
+452
+00:43:15,570 --> 00:43:19,270
+هي positive كتلازملكن لو ما اتكون اشي يبقى هي
+
+453
+00:43:19,270 --> 00:43:22,630
+negative catalase او بنحكي smooth suspension بيكون
+
+454
+00:43:22,630 --> 00:43:25,490
+ال suspension smooth مافي عندي اي اشي مافي عندي
+
+455
+00:43:25,490 --> 00:43:29,830
+air bubble هذا بالنسبة ل slide method ل tube
+
+456
+00:43:29,830 --> 00:43:34,210
+method نفس الفكرة بعد ما اضيف الواحد مل من ال
+
+457
+00:43:34,210 --> 00:43:37,470
+hydrogen peroxide بقولي بتكون air bubbles في ال
+
+458
+00:43:37,470 --> 00:43:40,130
+tube زي ما احنا ملاحظين يبقى هي high positive
+
+459
+00:43:40,130 --> 00:43:44,210
+catalase لكن هان ماما نلاحظ في عندي اي فقعات يبقى
+
+460
+00:43:44,210 --> 00:43:49,580
+هينفس الفكرة للسلايد ميثود نفس الفكرة فى اندي air
+
+461
+00:43:49,580 --> 00:43:53,200
+bubbles يبقى كتلاس بوزيتف لكن هن فى اندي smooth
+
+462
+00:43:53,200 --> 00:43:59,720
+suspension يبقى هن نيجاتف للكتلس هيك اللى هى ال
+
+463
+00:43:59,720 --> 00:44:00,100
+result
+
+464
+00:44:19,840 --> 00:44:24,560
+هذه الـ slide هنتكلم عن بعض الملاحظات الهمة بقول
+
+465
+00:44:24,560 --> 00:44:27,860
+لي be careful when using bacteria from blood agar
+
+466
+00:44:27,860 --> 00:44:30,860
+culture and avoid touching the agar by loop
+
+467
+00:44:30,860 --> 00:44:34,780
+because blood cells in agar also had catalase
+
+468
+00:44:34,780 --> 00:44:39,160
+enzyme بيعطيني falls positive بقول لي ممنوع أخد
+
+469
+00:44:39,160 --> 00:44:42,620
+كولونيه عشان أعمل فحص اللي هو ال catalase test من
+
+470
+00:44:42,620 --> 00:44:47,540
+blood agarقلنا البلد أجار هي Enriched Media منضيف
+
+471
+00:44:47,540 --> 00:44:52,240
+البلد اللى هو supplement يعتبر بنسبة 5% يعني لو
+
+472
+00:44:52,240 --> 00:44:56,400
+أنا حضرت 1 liter من ال nutrient أجار بضيف 50 ml من
+
+473
+00:44:56,400 --> 00:44:58,900
+البلد طبعا بعد ما أعاقم ال media و تطلع من ال
+
+474
+00:44:58,900 --> 00:45:02,920
+autoclave بستنها تبرد بعدين بضيف البلد عشان ما
+
+475
+00:45:02,920 --> 00:45:07,880
+يتكسر طيب ليش أنا ممنوع أخد كولوني من البلد أجار
+
+476
+00:45:08,150 --> 00:45:11,930
+بقولّي لو كنت زارعة بكتيريا على إبلاد أجار وعملتي
+
+477
+00:45:11,930 --> 00:45:15,970
+فحص الـ Catalase ممكن يعطيني false positive result
+
+478
+00:45:15,970 --> 00:45:20,830
+ليش؟ لأنك لما تاخدي كولوني من إبلاد أجار ممكن تروح
+
+479
+00:45:20,830 --> 00:45:25,350
+تاخدي مع الـ colony تجرح الأجار يعني تاخدي شوية
+
+480
+00:45:25,350 --> 00:45:30,690
+media يعني blood قولنا لـ blood cells نفسها بتحتوي
+
+481
+00:45:30,690 --> 00:45:35,610
+على Catalase enzyme بالتاليممكن البكتيريا ما يكون
+
+482
+00:45:35,610 --> 00:45:39,450
+عندها أنزيم الـ Catalase لكن انت أخدت كله عن طريق
+
+483
+00:45:39,450 --> 00:45:43,470
+أو من طبق ال blood agar و جرحت هذا ال agar يعني
+
+484
+00:45:43,470 --> 00:45:47,050
+أخدت blood اللي موجود فيه Catalase enzyme بالتالي
+
+485
+00:45:47,050 --> 00:45:51,910
+هياتفاعل مع ال substrate اللي احنا حاطينها ال H2O2
+
+486
+00:45:51,910 --> 00:45:56,710
+وبعطيني positive resultعشان هيك او بيعطيني false
+
+487
+00:45:56,710 --> 00:46:00,550
+positive result نتيجة خاطئة مش البكتيريا عندها ال
+
+488
+00:46:00,550 --> 00:46:03,670
+enzyme عشان هيك الأفضل اني انا اشتغل اي فحص
+
+489
+00:46:03,670 --> 00:46:07,550
+كيميائي على general media على nutrient agar لو
+
+490
+00:46:07,550 --> 00:46:11,830
+اضطريت اني انا ااخد من blood agar بحاول اني انا
+
+491
+00:46:11,830 --> 00:46:16,010
+ااخد بدون ما اجرح ال media avoid touching the agar
+
+492
+00:46:16,010 --> 00:46:20,520
+by the loopهذا بالنسبة للملاحظة الأولى الملاحظة
+
+493
+00:46:20,520 --> 00:46:23,480
+التانية also don't use bacteria from old culture
+
+494
+00:46:23,480 --> 00:46:27,320
+because the enzyme activity drop by time بيعطيني
+
+495
+00:46:27,320 --> 00:46:31,160
+false negative اي فحص كيميائي لازم اني انا اشتغله
+
+496
+00:46:31,160 --> 00:46:35,360
+تكون ال culture fresh لأنه لو كانت old culture
+
+497
+00:46:35,360 --> 00:46:39,060
+بتكون البكتيريا منهجة تمام ال enzyme activity
+
+498
+00:46:39,060 --> 00:46:44,770
+بيبدأ EL فبالتالي بيعطيني false negative resultآخر
+
+499
+00:46:44,770 --> 00:46:48,490
+ملاحظة احنا هنضيفها و احنا نحكيها قبل ما نختم بالـ
+
+500
+00:46:48,490 --> 00:46:52,750
+Catalase Test هلأ مش كل البكتيريا قلنا عندها
+
+501
+00:46:52,750 --> 00:46:54,990
+Enzyme الـ Catalase على سبيل المثال قلنا الـ
+
+502
+00:46:54,990 --> 00:46:58,770
+Streptococci ما عندها Enzyme الـ Catalase طب قلنا
+
+503
+00:46:58,770 --> 00:47:02,910
+خلال التنفس الخلوي بيتم إنتاج الـ H2O2 اللي هو
+
+504
+00:47:02,910 --> 00:47:07,690
+يعتبر toxic worst product كيف البكتيريا اللي ما
+
+505
+00:47:07,690 --> 00:47:11,570
+عندها Enzyme الـ Catalase هتتخلص من الـ H2O2 اللي
+
+506
+00:47:11,570 --> 00:47:14,680
+يعتبر toxicوقلنا ان البكتيريا اللى معاها عندها
+
+507
+00:47:14,680 --> 00:47:18,300
+انزيم الـ catalase عندها انزيم آخر بيقوم بنفس
+
+508
+00:47:18,300 --> 00:47:23,240
+الوظيفة اللى هو اسمه hydrogen peroxidase من اسمه
+
+509
+00:47:23,240 --> 00:47:25,740
+hydrogen peroxidase يعني بيكسر ال hydrogen
+
+510
+00:47:25,740 --> 00:47:29,580
+peroxide هيشتغل على نصف ال substrate اللى هى ال
+
+511
+00:47:29,580 --> 00:47:35,780
+H2O2 لكن هيعطينى جزيئان ميه هيعطينى two جزيئان
+
+512
+00:47:35,780 --> 00:47:40,780
+H2O2النواتج هتختلف هيعطيني جزيئان ميا مش هيعطيني
+
+513
+00:47:40,780 --> 00:47:45,320
+O2 فبالتالي مش هقدر انا استدل على التفاعل هذا زي
+
+514
+00:47:45,320 --> 00:47:51,260
+ال Catalase Test هذا بالنسبة لفحص Catalase Test
+
+515
+00:47:51,260 --> 00:47:54,520
+خلصناه هنتقل ل ال Coagulase Test
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/YZtUaTNI_xw_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/YZtUaTNI_xw_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..15b9b743d33ad43753329bf014deb8af5a3993e1
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/YZtUaTNI_xw_raw.srt
@@ -0,0 +1,2060 @@
+1
+00:00:01,960 --> 00:00:05,640
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:05,640 --> 00:00:09,080
+جميعًا في هذا اللقاء هنتكلم عن Catalase and
+
+3
+00:00:09,080 --> 00:00:13,620
+Coagulase Test قولنا إن البكتيريا Small and
+
+4
+00:00:13,620 --> 00:00:17,300
+Colorless اتغلبنا على إنها Small باستخدام
+
+5
+00:00:17,300 --> 00:00:20,520
+الميكروسكوب الميكروسكوب عملّي magnification
+
+6
+00:00:20,520 --> 00:00:25,320
+للبكتيريا وإقدرت إني أنا أشوفها بالنسبة لإنها
+
+7
+00:00:25,320 --> 00:00:30,080
+Colorless شفافة حلّت هذه المشكلة باستخدام الستان
+
+8
+00:00:30,080 --> 00:00:33,920
+باستخدام الصبغاطبتعرفنا على أنواع الصبغات اللي من
+
+9
+00:00:33,920 --> 00:00:38,360
+ضمنها لـ Gram Stain لـ Gram Stain كانت أول خطوة من
+
+10
+00:00:38,360 --> 00:00:42,180
+خطوات ال identification أول خطوة من خطوات تعريف
+
+11
+00:00:42,180 --> 00:00:47,320
+البكتيريا لـ Gram Stain قسمت البكتيريا لمجموعات لـ
+
+12
+00:00:47,320 --> 00:00:51,020
+Gram Positive Bacteria و Gram Negative Bacteria لـ
+
+13
+00:00:51,020 --> 00:00:55,100
+Gram Positive Bacteria بتضم مجموعة من البكتيريا و
+
+14
+00:00:55,100 --> 00:00:58,980
+كذلك لـ Gram Negative عشان أوصل للتعريف النهائي
+
+15
+00:00:59,210 --> 00:01:04,030
+لازم اعمل فحوصات كيميائية Biochemical Test واليوم
+
+16
+00:01:04,030 --> 00:01:09,130
+هنتكلم عن اول فحصين كيميائيين يهم ال Catalase and
+
+17
+00:01:09,130 --> 00:01:14,670
+Coagulase Test عشان نوضح فكرة الفحوصات الكيميائية
+
+18
+00:01:14,670 --> 00:01:20,030
+لو انت بدك تقابل بنت لأول مرة وعرفتي مواصفات هذه
+
+19
+00:01:20,030 --> 00:01:24,130
+البنت اكيد لما توصلي للمكان اللي هتتقابله فيه
+
+20
+00:01:24,130 --> 00:01:27,350
+وقتها تشوفي مين في المكان ليه نفس هذه المواصفات
+
+21
+00:01:27,350 --> 00:01:33,670
+وتستفنيلما توصلى لابنت اللى انت بدك اياها يبقى
+
+22
+00:01:33,670 --> 00:01:37,990
+جمعتى اكتر من صفة لهذه البنت عشان تعرفيها نفس
+
+23
+00:01:37,990 --> 00:01:41,950
+الشغل مع البكتيريا عشان اوصل للتعريف النهائى بدي
+
+24
+00:01:41,950 --> 00:01:46,550
+اجمع صفات وخصائص عشان اعرفها من خلال الفحوصات
+
+25
+00:01:46,550 --> 00:01:51,510
+الكيميائية شوف فكرة الفحوصات الكيميائية كل بكتيريا
+
+26
+00:01:51,510 --> 00:01:55,610
+عندها انزيمات معينة انا بدي اكشف هذه الانزيمات
+
+27
+00:01:55,610 --> 00:02:00,170
+موجودة او لأاحنا بنعرف إنه الانزيم بيشتغل على
+
+28
+00:02:00,170 --> 00:02:05,090
+substrate عشان أكشف هل الانزيم موجودة إن البكتيريا
+
+29
+00:02:05,090 --> 00:02:10,210
+أو لأ بجيب مادة كيميائية أقلد و أحاكي فيها ال
+
+30
+00:02:10,210 --> 00:02:13,090
+substrate اللي بيشتغل عليها الانزيم الموجود
+
+31
+00:02:13,090 --> 00:02:17,750
+بالبكتيريا ونستدل طبعا إن التفاعل صار أو لأ من
+
+32
+00:02:17,750 --> 00:02:22,230
+خلال نواتج التفاعل ممكن أشوف نواتج التفاعل بال
+
+33
+00:02:22,230 --> 00:02:28,290
+next I أو ممكن يكون تغير في لون ال mediaاليوم ان
+
+34
+00:02:28,290 --> 00:02:32,730
+شاء الله هنبدأ بأول فحص كيميائي الـ catalase test
+
+35
+00:02:32,730 --> 00:02:37,530
+لكن قبل ما نتكلم عن ال catalase and coagulase test
+
+36
+00:02:37,530 --> 00:02:46,870
+هنتكلم عن ال enzyme بشكل عام enzymes
+
+37
+00:02:46,870 --> 00:02:50,830
+in the microbiology lab biochemical test relies on
+
+38
+00:02:50,830 --> 00:02:55,100
+enzymeفي معمل الـ Microbiology الـ biochemical
+
+39
+00:02:55,100 --> 00:02:59,340
+test الفحوصات بتعتمد على الانزيمات which is
+
+40
+00:02:59,340 --> 00:03:04,120
+glycoprotein or protein that as catalyst by
+
+41
+00:03:04,120 --> 00:03:07,000
+lowering the activation energy of certain
+
+42
+00:03:07,000 --> 00:03:11,660
+biological reaction فيها تعريف مهم اللي هو ال
+
+43
+00:03:11,660 --> 00:03:16,570
+enzymeمعرف للإنزايم اللى هو ممكن انها تكون الـ
+
+44
+00:03:16,570 --> 00:03:21,590
+glycoprotein أو البروتين يبقى الإنزيمات ممكن تكون
+
+45
+00:03:21,590 --> 00:03:27,510
+ال glycoprotein بروتين مرتبط بوليغوسكاريد بشوجر أو
+
+46
+00:03:27,510 --> 00:03:33,090
+بروتين أو بروتين لحال بيشتغل as catalyst كمحفظ
+
+47
+00:03:34,880 --> 00:03:39,600
+بيشتغل كمحفز بيقلل طاقة التنشيط Lowering the
+
+48
+00:03:39,600 --> 00:03:43,760
+activation energy طيب لو بدنا نعرف طاقة التنشيط شو
+
+49
+00:03:43,760 --> 00:03:47,880
+هي ال activation energy طاقة التنشيط هو الحد
+
+50
+00:03:47,880 --> 00:03:52,620
+الأدنى من الطاقة اللازم لحدوث التفاعل يبقى أقل شئ
+
+51
+00:03:52,620 --> 00:03:56,600
+من الطاقة أنا بديه عشان يصير التفاعل بسميه ال
+
+52
+00:03:56,600 --> 00:04:01,810
+activation energyبالنسبة للمعادلة قلنا الإنزايم
+
+53
+00:04:01,810 --> 00:04:05,190
+هيشتغل على substrate ال substrate طبعا هي مواد
+
+54
+00:04:05,190 --> 00:04:10,290
+التفاعل عشان يعطيني product نستطيع استخدام علمنا
+
+55
+00:04:10,290 --> 00:04:15,870
+في الانزايم البكتيريا لنعرف البكتيريا ونقرر بين
+
+56
+00:04:15,870 --> 00:04:20,300
+مجموعات البكتيرياهدفنا من دراسة الإنزيمات
+
+57
+00:04:20,300 --> 00:04:24,420
+بالبكتيريا عشان أعمل identification و أميز
+
+58
+00:04:24,420 --> 00:04:29,260
+البكتيريا عن بعض زي ما وضحنا في بداية المحاضرة
+
+59
+00:04:29,260 --> 00:04:36,220
+types
+
+60
+00:04:36,220 --> 00:04:41,280
+of enzyme أنواع الإنزيمات الأنواع الإنزيمات تنقسم
+
+61
+00:04:41,280 --> 00:04:47,440
+حسبAccording to site of the reaction حسب موقع
+
+62
+00:04:47,440 --> 00:04:53,920
+العمل وحسب مكان أو حسب الإنتاج يبقى بقسم الإنزيمات
+
+63
+00:04:53,920 --> 00:04:57,780
+حسب موقع العمل ال site of reaction أو حسب ال
+
+64
+00:04:57,780 --> 00:05:03,440
+enzyme production حسب الإنتاج بالنسبة لموقع العمل
+
+65
+00:05:03,440 --> 00:05:08,580
+ال site of reaction بصنفها إلى endoenzyme and
+
+66
+00:05:08,580 --> 00:05:13,860
+exoenzyme يبقىالإنزيمات تنقسم على حسب ال site of
+
+67
+00:05:13,860 --> 00:05:18,820
+reaction إلى endoenzyme and exoenzyme بالنسبة لها
+
+68
+00:05:18,820 --> 00:05:22,840
+الـ endoenzyme بيصير التفاعل intracellular يبقى
+
+69
+00:05:22,840 --> 00:05:27,980
+بيصير التفاعل داخل الخلية where substrate and
+
+70
+00:05:27,980 --> 00:05:33,150
+enzyme react inside the cellبقول لي إن ال
+
+71
+00:05:33,150 --> 00:05:36,410
+substrate و ال enzyme هيتفاعلوا داخل الخلية ال
+
+72
+00:05:36,410 --> 00:05:41,150
+enzyme يفرز داخل الخلية بتفرز الخلية ال substrate
+
+73
+00:05:41,150 --> 00:05:45,270
+ممكن إنها تكون جوا الخلية و ممكن إنها تكون برا
+
+74
+00:05:45,270 --> 00:05:49,050
+الخلية هلأ لو كانت ال substrate داخل الخلية مافي
+
+75
+00:05:49,050 --> 00:05:52,730
+عندي مشكلة ال enzyme يفرز من داخل الخلية ال
+
+76
+00:05:52,730 --> 00:05:55,410
+substrate موجودة داخل الخلية التفاعل هيكون
+
+77
+00:05:55,410 --> 00:06:00,980
+intracellularلكن لو كانت الـ substrate خارج الخلية
+
+78
+00:06:00,980 --> 00:06:05,620
+والإنزيم أكيد هيتم إنتاجه داخل الخلية والإنزيم
+
+79
+00:06:05,620 --> 00:06:10,520
+Indoenzyme لازم التفاعل يصير Intracellular عشان
+
+80
+00:06:10,520 --> 00:06:15,820
+هيك الخلية هتفرز إنزيمات هذه الإنزيمات وظيفتها
+
+81
+00:06:15,820 --> 00:06:19,380
+أنها تدخل ال substrate لداخل الخلية عشان يصير
+
+82
+00:06:19,380 --> 00:06:25,410
+التفاعل Intracellularيبقى بالنسبة للـ Endoenzyme
+
+83
+00:06:25,410 --> 00:06:28,590
+الـ enzyme يفرز داخل الخلية ال substrate ممكن تكون
+
+84
+00:06:28,590 --> 00:06:32,230
+داخل الخلية وممكن تكون خارج الخلية إذا كانت داخل
+
+85
+00:06:32,230 --> 00:06:35,570
+الخلية مافي عندى اي مشكلة لو كانت خارج الخلية
+
+86
+00:06:35,570 --> 00:06:39,510
+وقتها الخلية هتفرز انزيمات هذه الانزيمات وضيفتها
+
+87
+00:06:39,510 --> 00:06:42,370
+انها تدخل ال substrate لجوا الخلية عشان يصير
+
+88
+00:06:42,370 --> 00:06:47,570
+التفاعل داخل الخلية من الأمثلة على الـ Endoenzyme
+
+89
+00:06:47,570 --> 00:06:53,810
+أكسيداز كتلاز بيورياز نيترات ريدكتازطبعا هدول حفظ
+
+90
+00:06:53,810 --> 00:06:57,630
+هنتعرف عليهم في المعامل اللي جاي هناخد كل الفحص و
+
+91
+00:06:57,630 --> 00:07:01,930
+نتعرف على ال principle لكل الفحص و كيف بنشتغل هذا
+
+92
+00:07:01,930 --> 00:07:07,870
+الفحص بالنسبة لـ Exoenzyme الـ Exoenzyme مكان حدوث
+
+93
+00:07:07,870 --> 00:07:13,670
+التفاعل خارج الخلية extra cellular where substrate
+
+94
+00:07:13,670 --> 00:07:18,290
+and enzyme react outside the cell بقول ال enzyme و
+
+95
+00:07:18,290 --> 00:07:23,330
+ال substrate هيتفاعلوا خارج الخليةالانزايم برضه
+
+96
+00:07:23,330 --> 00:07:28,170
+هتفرز الخلية هيفرز داخل الخلية لكن ال substrate
+
+97
+00:07:28,170 --> 00:07:32,130
+هتكون فقط خارج الخلية احنا قلنا في ال endoenzyme
+
+98
+00:07:32,130 --> 00:07:36,210
+ممكن تكون داخل وممكن تكون خارج لكن في ال exoenzyme
+
+99
+00:07:36,210 --> 00:07:40,990
+ال substrate هتكون خارج الخليةما بقدر أدخل الـ
+
+100
+00:07:40,990 --> 00:07:43,970
+Substrate زي ما عملت في الـ Endoenzyme لداخل
+
+101
+00:07:43,970 --> 00:07:48,290
+الخلية لأن حجم الـ Substrate كبير عشان كده الـ
+
+102
+00:07:48,290 --> 00:07:53,490
+Enzyme بيطلع لبرا يبقى الـ Exoenzyme الـ Enzyme
+
+103
+00:07:53,490 --> 00:07:57,230
+بيفرز داخل الخلية بيطلع لبرا عند الـ Substrate لأن
+
+104
+00:07:57,230 --> 00:08:01,570
+الـ Substrate حجمها كبير و بيصير التفاعل بين الـ
+
+105
+00:08:01,570 --> 00:08:06,130
+Enzyme والـ Substrate خارج الخلية من الأمثلة على
+
+106
+00:08:06,130 --> 00:08:11,180
+الـ Exoenzyme وحفظهPreco-adiolase جيلاتيناز أميلاز
+
+107
+00:08:11,180 --> 00:08:16,360
+ليباز كازيناز يبقى هدول أمثلة على الـ Exoenzyme
+
+108
+00:08:16,360 --> 00:08:20,240
+يبقى هذا بالنسبة للـ site of reaction قسمنا
+
+109
+00:08:20,240 --> 00:08:25,320
+الإنزيمات لإندوإنزايم and إكزوإنزايم بالنسبة
+
+110
+00:08:25,320 --> 00:08:30,780
+للإنزايم production موقع أو حسب الإنتاج حسب
+
+111
+00:08:30,780 --> 00:08:34,520
+الإنتاج بقسمه لinducible and constitutive
+
+112
+00:08:34,520 --> 00:08:40,300
+inducibleوconstitutive شو الفرق بينهم؟ بقولي الـ
+
+113
+00:08:40,300 --> 00:08:45,120
+inducible بكون عندي محفظ يتم إنتاج ال enzyme فقط
+
+114
+00:08:45,120 --> 00:08:50,260
+في وجود محفظ المحفظ هيحفظ هذا ال enzyme produce
+
+115
+00:08:50,260 --> 00:08:56,760
+only when needed or induced فقط عندما يكون المحفظ
+
+116
+00:08:56,760 --> 00:09:02,040
+يتم إنتاج هذا ال enzyme لكن الconstitutive بيتم
+
+117
+00:09:02,040 --> 00:09:07,590
+إنتاجه بشكل مستمر continuouslyبشكل مستمر بغض النظر
+
+118
+00:09:07,590 --> 00:09:13,350
+فيه عندي محفز أو لأ هذا بالنسبة لأنواع الانزيم حسب
+
+119
+00:09:13,350 --> 00:09:17,050
+الانزيم ال production حسب الإنتاج يبقى type of
+
+120
+00:09:17,050 --> 00:09:20,550
+enzyme حسب ال site of reaction انسو انزيم و اندو
+
+121
+00:09:20,550 --> 00:09:25,610
+انزيم حسب الانزيم ال production ل inducible و
+
+122
+00:09:25,610 --> 00:09:26,690
+constitutive
+
+123
+00:09:36,140 --> 00:09:40,920
+notes ملاحظات مهمة every genus of bacteria has its
+
+124
+00:09:40,920 --> 00:09:44,880
+unique set of enzymes so we can identify it بقول
+
+125
+00:09:44,880 --> 00:09:48,840
+لي كل مجموعة من البكتيريا إلها أنزيمات خاصة فيها
+
+126
+00:09:48,840 --> 00:09:54,320
+هذا بيساعدني أني أنا أعمل identification للبكتيريا
+
+127
+00:09:54,320 --> 00:09:59,300
+على سبيل المثال ال gram positive cocci بتضم
+
+128
+00:09:59,300 --> 00:10:04,460
+لستريبتو cocci و لستافيلو cocciاستفيلوكوكاي عندها
+
+129
+00:10:04,460 --> 00:10:08,720
+انزيم الكتالاز لكن الاستريبتوكوكاي ما عندها انزيم
+
+130
+00:10:08,720 --> 00:10:12,840
+الكتالاز بروح انا بشتغل فحص الكتالاز اذا طلعت
+
+131
+00:10:12,840 --> 00:10:17,240
+كتالاز negative يبقى بحكي انه هي استريبتوكوكاي هي
+
+132
+00:10:17,240 --> 00:10:21,700
+قدرت انا اعمل identification من خلال الانزيمات من
+
+133
+00:10:21,700 --> 00:10:26,980
+خلال وجود انزيمات في بكتيريا معينة وعدم وجودها في
+
+134
+00:10:26,980 --> 00:10:33,090
+بكتيريا اخرىIndoenzyme may act outside the cell in
+
+135
+00:10:33,090 --> 00:10:36,250
+case of the presence of high concentration of its
+
+136
+00:10:36,250 --> 00:10:41,530
+substrate اتفقنا إنه الـIndoenzyme بيفرز داخل
+
+137
+00:10:41,530 --> 00:10:45,690
+الخلية و بيظل داخلها، ما بيطلع برا و بيصير التفاعل
+
+138
+00:10:45,690 --> 00:10:48,510
+داخل الخلية، لو كانت ال substrate برا هي اللي
+
+139
+00:10:48,510 --> 00:10:52,730
+بتدخل لجوة فهذه الجملة بيقول لي في عندي استثناء،
+
+140
+00:10:52,730 --> 00:10:58,330
+احنا قلنا إنه مافي إش عندي absolute أكيد لكل قاعدة
+
+141
+00:10:58,330 --> 00:11:04,580
+شوادممكن الـ Endoenzyme يطلع برا الخلية في حالة
+
+142
+00:11:04,580 --> 00:11:09,720
+واحدة فقط لو كان تركيز الـ substrate خارج الخلية
+
+143
+00:11:09,720 --> 00:11:15,080
+أكتر بكتير من تركيزه في الداخل عشان هيك الـ enzyme
+
+144
+00:11:15,080 --> 00:11:18,880
+هتفرز الخلية ويطلع لبرا عند التركيز العالي من ال
+
+145
+00:11:18,880 --> 00:11:23,980
+substrate ويصير التفاعل هذا بالنسبة للملاحظة
+
+146
+00:11:23,980 --> 00:11:29,840
+التانية الـ Endoenzyme ممكن أنهيطلع خارج الخلية في
+
+147
+00:11:29,840 --> 00:11:33,120
+حال كان عندي الـ Concentration of its substrate
+
+148
+00:11:33,120 --> 00:11:38,440
+تركيزه خارج الخلية أعلى من داخل الخلية آخر ملاحظة
+
+149
+00:11:38,440 --> 00:11:42,840
+The same enzyme could be inducible and constitute
+
+150
+00:11:42,840 --> 00:11:48,320
+in different genera كاتب مثال اليورياسي أحنا قلنا
+
+151
+00:11:48,320 --> 00:11:52,800
+أن الإنزايم يقسم حسب الإنتاج إلى inducible and
+
+152
+00:11:52,800 --> 00:11:59,140
+constitutiveهذه الجملة تقول أن الانزيم نفسه مثلا X
+
+153
+00:11:59,140 --> 00:12:03,640
+ممكن يكون inducible في بكتيريا معينة وconstitute
+
+154
+00:12:03,640 --> 00:12:08,340
+في بكتيريا أخرى من الأمثلة على هذا الانزيم اللي
+
+155
+00:12:08,340 --> 00:12:12,000
+ممكن يكون inducible في بكتيريا وconstitute في
+
+156
+00:12:12,000 --> 00:12:16,960
+بكتيريا أخرى اليوريازي اليورياز من اسمه يورياز
+
+157
+00:12:16,960 --> 00:12:22,230
+هيكسر اليوريااليوريا طبعا هي toxic waste product
+
+158
+00:12:22,230 --> 00:12:27,010
+نتخلص منها عن طريق تكسيرها ل ammonia and CO2
+
+159
+00:12:27,010 --> 00:12:34,910
+بالنسبة لليورياز هيكسر لي اليوريا ل ammonia and
+
+160
+00:12:34,910 --> 00:12:43,190
+CO2 فلو كان بالنسبة لل inducible في بكتيريا اسمها
+
+161
+00:12:43,190 --> 00:12:48,370
+Brutias بتفرز أنزيم اليورياز في موجود محفظ يعني
+
+162
+00:12:49,580 --> 00:12:53,320
+بنعتبرها inducible لما نعتبر الإنزايم HAN
+
+163
+00:12:53,320 --> 00:12:58,080
+inducible لكن في حال عدم وجود المحفظ لا يتم إنتاجه
+
+164
+00:12:58,080 --> 00:13:02,360
+يبقى HAN كان الإنزيم اليوريز inducible في
+
+165
+00:13:02,360 --> 00:13:07,740
+البروتياس فقط في وجود محفظ هيتم إنتاجه لكن فيه
+
+166
+00:13:07,740 --> 00:13:12,400
+اندباتكيريا الهيليكوباكتر بيلوري يتم إنتاجه بشكل
+
+167
+00:13:12,400 --> 00:13:18,610
+مستمر يعني يعتبر constitutiveالـ H-Biolary بتكون
+
+168
+00:13:18,610 --> 00:13:23,030
+في المعدة طريقة الـ infection تقليل حموضة المعدة
+
+169
+00:13:23,030 --> 00:13:28,450
+كيف؟ بتظهر تفرز إنزايم اليورياز عشان تتكسر اليوريا
+
+170
+00:13:28,450 --> 00:13:33,210
+وتنتج الأمانيا الأمانيا طبعا قاعدة هيرتفع الـ pH
+
+171
+00:13:33,210 --> 00:13:38,490
+وتتقلل من حموضة المعدة يبقى الإنزايم نفسه اليورياز
+
+172
+00:13:38,490 --> 00:13:43,390
+ممكن يكون inducible وممكن يكون constitutive ذكرنا
+
+173
+00:13:43,390 --> 00:13:49,400
+مثل اليورياز إنزايمكان Inducable في الـ Brutias
+
+174
+00:13:49,400 --> 00:13:54,540
+فقط في موجود محفظ هيتم إنتاجه وكان Constitutive في
+
+175
+00:13:54,540 --> 00:13:59,600
+الـ H-Biliary في الـ الـ H-Biliary الـ
+
+176
+00:13:59,600 --> 00:14:02,960
+Helicobacter Biliary كان Constitutive هيتم إنتاجه
+
+177
+00:14:02,960 --> 00:14:06,280
+بشكل مستمر لأن طريقة الـ Infection اللي هو تقليل
+
+178
+00:14:06,280 --> 00:14:11,280
+من حموضة المعدة لازم يتم إنتاج Ammonia أو مركب
+
+179
+00:14:11,280 --> 00:14:15,950
+قاعدي هيرتفع الـ pH وتتقلل حموضة المعدةهذا بالنسبة
+
+180
+00:14:15,950 --> 00:14:23,110
+لآخر ملاحظة kinds
+
+181
+00:14:23,110 --> 00:14:27,010
+of bacterial enzymatic reaction أنواع الـ
+
+182
+00:14:27,010 --> 00:14:30,590
+enzymatic reaction أو ليش البكتيريا بتفرز ال
+
+183
+00:14:30,590 --> 00:14:37,010
+enzyme أول نوع the breakdown of toxic wastesuch as
+
+184
+00:14:37,010 --> 00:14:41,390
+a hydrogen peroxide or urea بقول ليه بتتخلص منه ال
+
+185
+00:14:41,390 --> 00:14:46,170
+worst product زي ال urea and hydrogen peroxide ال
+
+186
+00:14:46,170 --> 00:14:52,270
+H2O2 طبعا هدول ال H2O2 و ال urea يعتبروا toxic
+
+187
+00:14:52,270 --> 00:14:57,110
+طبعا بيتم يهم فضلات بيتم إنتاجهم من عمليات عيضية
+
+188
+00:14:57,110 --> 00:15:01,430
+عشان أتخلص من هذه الفضلات بيتم إفراز أنزيم هذه
+
+189
+00:15:01,430 --> 00:15:06,530
+الانزيمالانزيمات بتتخلص من هذه الـ toxic material
+
+190
+00:15:06,530 --> 00:15:12,190
+عشان أتخلص من اليوريا بتفرز البكتيريا انزيم
+
+191
+00:15:12,190 --> 00:15:16,810
+اليورياز عشان أتخلص من الـ hydrogen peroxide الـ
+
+192
+00:15:16,810 --> 00:15:23,570
+H2O2 بتفرز البكتيريا انزيم الكتلازي هذا بالنسبة
+
+193
+00:15:23,570 --> 00:15:27,610
+لأول نوع من ال enzymatic reaction اللي هو أني أنا
+
+194
+00:15:27,610 --> 00:15:31,300
+أتخلص من الـ toxic materialالتاني نوع The
+
+195
+00:15:31,300 --> 00:15:37,100
+reduction of Nitrate or Oxygen اختزال للنيترات او
+
+196
+00:15:37,100 --> 00:15:40,900
+الأكسجين مثال عليها الـ Nitrate Reductase من اسمه
+
+197
+00:15:40,900 --> 00:15:44,840
+Nitrate Reductase هي عمل Reduction للنيترات
+
+198
+00:15:44,840 --> 00:15:49,940
+بالنهاية عشان احصل على ال nitrogen source اللازم
+
+199
+00:15:49,940 --> 00:15:54,730
+لنمو البكتيرياتالت نوع الـ degradation of specific
+
+200
+00:15:54,730 --> 00:15:59,530
+amino acid بيكسّل لبعض الأحماض الأمينية زي الـ
+
+201
+00:15:59,530 --> 00:16:03,630
+tryptophan الـ tryptophan amino acid بيتم تكسيره
+
+202
+00:16:03,630 --> 00:16:07,810
+بواسطة أنزيم الـ tryptophanase تمام، عشان أحصل
+
+203
+00:16:07,810 --> 00:16:12,370
+بالنهاية على carbon and nitrogen source لازم لـ
+
+204
+00:16:12,370 --> 00:16:17,390
+growth، لنموء البكتيريا رابع نوع الـ utilization
+
+205
+00:16:17,390 --> 00:16:22,040
+of non-carbohydrate carbon source for growthfor
+
+206
+00:16:22,040 --> 00:16:27,400
+example اليورياز بقوللي استهلاك non carbohydrate
+
+207
+00:16:27,400 --> 00:16:32,840
+source اللازم لنمو البكتيريا زي اليورياز حتى أحصل
+
+208
+00:16:32,840 --> 00:16:37,480
+على nitrogen and carbon source عشان تنمو البكتيريا
+
+209
+00:16:37,480 --> 00:16:41,760
+بحتاج ل carbon source و nitrogen source ال carbon
+
+210
+00:16:41,760 --> 00:16:45,220
+source ممكن تكون مصدرها ال carbohydrate أو المصدر
+
+211
+00:16:45,220 --> 00:16:48,590
+الأول لإلها ال carbohydrateفي حال الـ Carbohydrate
+
+212
+00:16:48,590 --> 00:16:53,010
+خلصت بروح لمصادر أخرى اللي هي الـ Non-Carbohydrate
+
+213
+00:16:53,010 --> 00:16:56,230
+Carbon Source من الأمثلة على الـ Non-Carbohydrate
+
+214
+00:16:56,230 --> 00:17:01,730
+Carbon Source زي اليوريا السيطرات عشان أستهلك
+
+215
+00:17:01,730 --> 00:17:06,690
+اليوريا والسيطرات أكيد في عندي انزيمات من الأمثلة
+
+216
+00:17:06,690 --> 00:17:10,550
+على الانزيمات اليورياز اليورياز هيكسرلي اليوريا
+
+217
+00:17:10,550 --> 00:17:14,650
+وأقدر ان احصل انا على Carbon Source كذلك السيطرات
+
+218
+00:17:15,360 --> 00:17:20,920
+كمان يُعتبر مثال على الـ Utilization of non
+
+219
+00:17:20,920 --> 00:17:24,740
+-carbohydrate carbon source هو مصدر للكربون لكن
+
+220
+00:17:24,740 --> 00:17:29,160
+non-carbohydrate مش من الكربوهيدرات مثال عليه الـ
+
+221
+00:17:29,160 --> 00:17:34,200
+Ureas هذا النوع و الـ citrate هذا بالنسبة للـ
+
+222
+00:17:34,200 --> 00:17:37,500
+bacterial enzymatic reaction إذا ملاحظ الـ Ureas
+
+223
+00:17:37,500 --> 00:17:44,240
+يُعتبر إله نوعين أو إله هدفين إن أنا أتخلص من الـ
+
+224
+00:17:44,240 --> 00:17:48,610
+toxicWest اللي هي اليوريا و بنفس الوقت بدي أحصل
+
+225
+00:17:48,610 --> 00:17:53,050
+على non carbohydrate carbon source يبقى اليورياز
+
+226
+00:17:53,050 --> 00:17:58,310
+كان في النوع الأول و النوع الرابع خلصنا ال
+
+227
+00:17:58,310 --> 00:18:04,410
+enzymatic reaction هلأ هنبدأ في أول نوع أو أول
+
+228
+00:18:04,410 --> 00:18:10,810
+enzyme اللي هو الكتلازب اسم ال enzyme طبعا من اسم
+
+229
+00:18:10,810 --> 00:18:15,290
+الفحص اللي هو الكتلازبالـ substrate اللي هيشتغل
+
+230
+00:18:15,290 --> 00:18:19,790
+عليها الـ enzyme الـ hydrogen peroxide الـ H2O2
+
+231
+00:18:19,790 --> 00:18:25,270
+اللي قولنا هي toxic material الـ enzyme action
+
+232
+00:18:25,270 --> 00:18:30,850
+break down the toxic H2O2 producing oxygen, gas
+
+233
+00:18:30,850 --> 00:18:36,230
+and water بيقوللي هيكسر ال toxic H2O2 قولنا ان الـ
+
+234
+00:18:36,230 --> 00:18:40,530
+H2O2 الـ hydrogen peroxide يعتبر toxic waste
+
+235
+00:18:40,530 --> 00:18:46,150
+productهيتكسر لـ O2 Gas and Water المعادلة مضحة
+
+236
+00:18:46,150 --> 00:18:50,050
+تفاعل الـ H2O2 قلنا الـ Substrate الإنزايم الـ
+
+237
+00:18:50,050 --> 00:18:54,650
+Catalase نواتج التفاعل O2 الـ Gas and Water الـ
+
+238
+00:18:54,650 --> 00:19:00,550
+H2O2 قلنا من أنواع الـ enzymatic reaction إنه يكسر
+
+239
+00:19:00,550 --> 00:19:05,110
+لي ال worst product الناتج من العمليات الأيضية ال
+
+240
+00:19:05,110 --> 00:19:10,830
+Catalase هيكسر الـ H2O2 الناتج من عمليةالتنفس
+
+241
+00:19:10,830 --> 00:19:17,090
+الخلوي الـ aerobic respiration يبقى الـ H2O2 هيتم
+
+242
+00:19:17,090 --> 00:19:20,750
+إنتاجه خلال عملية التنفس الخلوي الـ aerobic
+
+243
+00:19:20,750 --> 00:19:26,630
+respiration كيف بيتم إنتاج الـ H2O2 في عملية
+
+244
+00:19:26,630 --> 00:19:32,010
+التنفس الخلوي في انديانة electron transport chain
+
+245
+00:19:38,180 --> 00:19:41,240
+في عندي انات، electron
+
+246
+00:20:08,540 --> 00:20:13,180
+في عندنا electron transport chain بيتم التقال
+
+247
+00:20:13,180 --> 00:20:17,340
+الإلكترون من جزء لجزء حتى يوصل للمستقبل النهائي
+
+248
+00:20:17,340 --> 00:20:21,420
+للإلكترونات المستقبل النهائي للإلكترونات هو
+
+249
+00:20:21,420 --> 00:20:27,680
+الأكسجين هيرتبط الإلكترون بالمداري الأخر للأكسجين
+
+250
+00:20:27,680 --> 00:20:30,260
+حسب المعادلة التالية
+
+251
+00:20:41,340 --> 00:20:55,740
+طبعا هذا ال Oxygen يبقى
+
+252
+00:20:55,740 --> 00:20:59,280
+هيرتبط ال Electron خلال انتقاله في ال Electronic
+
+253
+00:20:59,280 --> 00:21:03,460
+Transport لكي ينتقل بيه للمستقبل أو هيرتبط بيه
+
+254
+00:21:03,460 --> 00:21:07,120
+المستقبل النهائي للإلكترونات اللي هو ال Oxygen هو
+
+255
+00:21:07,120 --> 00:21:09,840
+يعطيني ال Super Oxide
+
+256
+00:21:29,030 --> 00:21:38,570
+هيعطيني ال super oxide هذا بسميه ال
+
+257
+00:21:38,570 --> 00:21:47,030
+super
+
+258
+00:21:47,030 --> 00:21:58,170
+oxide او ال free radical ممكن نسميه هذا
+
+259
+00:21:58,170 --> 00:22:01,870
+ال superالـ Oxide أو الـ Free Radical يعتبر طبعا
+
+260
+00:22:01,870 --> 00:22:07,150
+Toxic Unstable طبعا هنتخلص منه في عندي في ال
+
+261
+00:22:07,150 --> 00:22:12,070
+Electronic Transport اتشان في عندي مضخات لـ H Plus
+
+262
+00:22:12,070 --> 00:22:16,550
+تمام الـ H Plus فبالتالي الـ Super Oxide هذا
+
+263
+00:22:16,550 --> 00:22:22,270
+هيرتبط بالـ H Plus ويعطيني الـ Hydrogen Peroxide
+
+264
+00:22:22,270 --> 00:22:23,850
+نكمل المعادلة
+
+265
+00:22:33,320 --> 00:22:37,780
+ال super oxide هذا unstable free radical toxic
+
+266
+00:22:37,780 --> 00:22:42,040
+يعتبر عشان اتخلص منه خلال او موجود فيه ال
+
+267
+00:22:42,040 --> 00:22:51,540
+electronic transport اتشان في عندي ال H plus ال
+
+268
+00:22:51,540 --> 00:22:56,260
+H plus بواسطة انزيم ال super oxide ديسميوتاز
+
+269
+00:22:56,260 --> 00:23:00,980
+هيتحول ل ال H2O2 اللي هو ال hydrogen peroxide
+
+270
+00:23:39,680 --> 00:23:45,400
+وسط انزيم السوبر اوكسايد دي سيميوتاز هيتحول لـ H2O
+
+271
+00:23:45,400 --> 00:23:54,740
+اللي
+
+272
+00:23:54,740 --> 00:24:00,020
+هو الهيدروجين بيروكسايد طبعا
+
+273
+00:24:00,020 --> 00:24:03,680
+ممكن هنوزن المعادلة اللي هي هاندفع اتنين
+
+274
+00:24:11,990 --> 00:24:17,130
+هكذا هذا هو الهيدروجين بيروكسايد هيك اتكون
+
+275
+00:24:17,130 --> 00:24:22,870
+الهيدروجين بيروكسايد هيتكسر لجاز and water بواسطة
+
+276
+00:24:22,870 --> 00:24:25,970
+انزيم الكتالاز هنتخلص الهيدروجين بيروكسايد يعتبر
+
+277
+00:24:25,970 --> 00:24:31,050
+toxic ل ال DNA و ل ال cell membrane عشان نتخلص منه
+
+278
+00:24:31,050 --> 00:24:35,850
+هيتم افراز انزيم الكتالاز الكتالاز هيتكسر ليا ل O2
+
+279
+00:24:35,850 --> 00:24:41,910
+and H2O لwater يبقىفي الـ Electron Transport Chain
+
+280
+00:24:41,910 --> 00:24:46,690
+في عندي الإلكترونات هتنتقل من جزء للجزء حتى تصل
+
+281
+00:24:46,690 --> 00:24:50,210
+للمستقبل إنها اللي هو الـ Oxygen هترتبط بالـ
+
+282
+00:24:50,210 --> 00:24:54,050
+Oxygen في المداري الأخير هتعطيني الـ Super Oxide
+
+283
+00:24:54,050 --> 00:25:00,250
+لـ Free Radical الـ R2-هذا الـ Free Radical
+
+284
+00:25:00,250 --> 00:25:05,430
+Unstable هيرتبط بالـ H Plus هاي الـ H Plus موجود
+
+285
+00:25:05,430 --> 00:25:08,550
+طبعا فيها اندي مضخات في الـ Electron Transported
+
+286
+00:25:08,550 --> 00:25:11,510
+Channel اللي هترتبط فيها بواسطة انزيم الـ Super
+
+287
+00:25:11,510 --> 00:25:16,070
+Oxide Dismutase هيعطيني الـ Hydrogen Peroxide هذا
+
+288
+00:25:16,070 --> 00:25:21,030
+بالنسبة لكيف تم إنتاج الـ H2O2 من عملية التنفس
+
+289
+00:25:21,030 --> 00:25:21,870
+الخلوي
+
+290
+00:25:25,820 --> 00:25:30,580
+بقول لي إن الـ hydrogen peroxide طبعا لازم يتكسر
+
+291
+00:25:30,580 --> 00:25:35,240
+to have to be breakdown to prevent its toxic
+
+292
+00:25:35,240 --> 00:25:39,580
+action عشان أمنع ال toxic action on DNA and cell
+
+293
+00:25:39,580 --> 00:25:44,200
+membrane قولنا هو toxic لل DNA and cell membrane
+
+294
+00:25:51,590 --> 00:25:55,170
+طيب كيف بدي أكشف هل البكتيريا عندها انزايم
+
+295
+00:25:55,170 --> 00:25:59,930
+الكتالاز او لأ قلنا بنجيب مادة أحكي فيها ال
+
+296
+00:25:59,930 --> 00:26:03,290
+substrate اللي بيشتغل عليها ال enzyme احنا قلنا ان
+
+297
+00:26:03,290 --> 00:26:07,970
+ال substrate اللي بيشتغل عليها الكتالاز هي ال H2O2
+
+298
+00:26:07,970 --> 00:26:11,910
+ال hydrogen peroxide بنجيب مادة كيميائية من
+
+299
+00:26:11,910 --> 00:26:18,510
+المختور اللي هي ال hydrogen peroxide 3% H2O2 بنضيف
+
+300
+00:26:18,510 --> 00:26:23,390
+عليه كولونيهذه الكولونية طبعاً إذا كانت الكتلاز
+
+301
+00:26:23,390 --> 00:26:28,170
+البكتيريا موجود فيها انزيم الكتلاز هيتكسر بواسطة
+
+302
+00:26:28,170 --> 00:26:33,090
+الانزيم وينتج Oxygen ال Oxygen بشوفه على شكل air
+
+303
+00:26:33,090 --> 00:26:36,590
+bubble يبقى when hydrogen peroxide is added to a
+
+304
+00:26:36,590 --> 00:26:39,630
+colony of كتلاز producing بكتيريا لو أنا ضفت ال
+
+305
+00:26:39,630 --> 00:26:44,820
+hydrogen peroxideالـ hydrogen peroxide اللي هو الـ
+
+306
+00:26:44,820 --> 00:26:49,700
+substrate 3% H2O توضفته للكولونيه للبكتيريا اللي
+
+307
+00:26:49,700 --> 00:26:54,580
+بتفرز الكتاليز أكيد هيتكسر لأكسجين، الأكسجين بقدر
+
+308
+00:26:54,580 --> 00:26:57,580
+أني أنا أكشف عنه من خلال أني أنا بشوفه على شكل
+
+309
+00:26:57,580 --> 00:27:02,320
+bubbles significance، significance هتمر معناه
+
+310
+00:27:02,320 --> 00:27:05,920
+كتير، كل فحص كيميائي لازم يكون فيه له significance
+
+311
+00:27:05,920 --> 00:27:10,190
+أو لازم نعرف شو ال significance لهذا الفحصالـ
+
+312
+00:27:10,190 --> 00:27:14,170
+Significance يعني شو أهمية الفحص بيفرق بين مين
+
+313
+00:27:14,170 --> 00:27:18,050
+ومين مين الـ Positive و الـ Negative لهذا الفحص
+
+314
+00:27:18,050 --> 00:27:23,930
+قبل من نتكلم على هاي ال slide بدنا نرسم نبدأ من
+
+315
+00:27:23,930 --> 00:27:33,790
+الـ Gram Stain لو أنا عملت نرسم Diagram عملت
+
+316
+00:27:33,790 --> 00:27:34,970
+Gram Stain
+
+317
+00:27:53,530 --> 00:27:56,590
+قلنا لـ Gram Stain بتقسم البكتيريا لـ Gram
+
+318
+00:27:56,590 --> 00:27:58,070
+Positive و Gram Negative
+
+319
+00:28:35,260 --> 00:28:39,080
+يبقى لجرام 2 تقسم للبكتيريا لجرام positive و لجرام
+
+320
+00:28:39,080 --> 00:28:42,380
+negative كمان لجرام positive و لجرام negative
+
+321
+00:28:42,380 --> 00:28:45,980
+تتقسم لإنها يصابغة لككاي و بصلاي
+
+322
+00:29:24,040 --> 00:29:41,880
+Okay بس ال line بس
+
+323
+00:29:41,880 --> 00:29:42,260
+ال line
+
+324
+00:29:54,170 --> 00:29:57,930
+يبقى بالنهاية قسمتلي إلى gram positive كوكاي gram
+
+325
+00:29:57,930 --> 00:30:01,350
+positive بصل لاي gram negative كوكاي gram negative
+
+326
+00:30:01,350 --> 00:30:06,470
+بصل لاي قلنا ان كل مجموع بتضم مثلا ل gram positive
+
+327
+00:30:06,470 --> 00:30:10,130
+كوكاي بتضم مجموعة من البكتيريا ل gram positive بصل
+
+328
+00:30:10,130 --> 00:30:13,570
+لاي بتضم مجموعة من البكتيريا كيف بدي افرق بينهم
+
+329
+00:30:13,570 --> 00:30:17,980
+باستخدام ال biochemical testالجرام بوزيتف بكتيريا
+
+330
+00:30:17,980 --> 00:30:21,980
+بتضم الاستريبتو كوكاي الاستافيلو كوكاي الماكرو
+
+331
+00:30:21,980 --> 00:30:28,020
+كوكاي والمايكرو كوكاي بقوللي عشان افرق بين هدال
+
+332
+00:30:28,020 --> 00:30:33,980
+المجموعة بعمل فحص الكتلاز طبعا يفقه لو طلعت عملت
+
+333
+00:30:33,980 --> 00:30:37,280
+جرامستان وطلعت معايا جرام بوزيتف كوكاي على طول
+
+334
+00:30:37,280 --> 00:30:43,150
+بعمل فحص الكتلازعشان افرق بين الستريبتو كوكاي
+
+335
+00:30:43,150 --> 00:30:46,790
+وباقي الأنواع الاستفيلو والماكرو والمايكرو يبقى
+
+336
+00:30:46,790 --> 00:30:49,890
+الخطوة التانية اني انا بدي اعمل ال catalase test
+
+337
+00:31:24,380 --> 00:31:39,100
+الـ Catalase Test لو
+
+338
+00:31:39,100 --> 00:31:41,780
+عملت ال Catalase Test وطلع Negative
+
+339
+00:31:57,390 --> 00:32:00,230
+يبقى البكتيريا هي Striptococcal
+
+340
+00:32:45,870 --> 00:32:49,830
+هذا بالنسبة لكتلازن ال test لو طلعت عملت الفحص و
+
+341
+00:32:49,830 --> 00:32:53,910
+طلعت negative يبقى هي streptococci لكن لو طلعت
+
+342
+00:32:53,910 --> 00:33:03,410
+positive في عندي تلت احتمالات اما Staphylococci
+
+343
+00:33:03,410 --> 00:33:13,670
+Staphylococci او macrococci او micrococci او
+
+344
+00:33:13,670 --> 00:33:14,270
+micro
+
+345
+00:33:28,760 --> 00:33:34,220
+Micrococci Staphylococci Macrococci
+
+346
+00:33:34,220 --> 00:33:38,000
+Micrococci يبقى هي positive يبقى فحص الكتلاز فرق
+
+347
+00:33:38,000 --> 00:33:41,060
+اللي بين الاستربتو cocci وما بين الاستفيلو
+
+348
+00:33:41,060 --> 00:33:46,130
+والماكرو والمايكرو cocciالـ Gram Positive Bacilli
+
+349
+00:33:46,130 --> 00:33:52,390
+برضه بتضم البصلص والكلستريديوم البصلص لو انا عملت
+
+350
+00:33:52,390 --> 00:33:57,270
+فحص الكتلاز البصلص هو positive والكلستريديوم
+
+351
+00:33:57,270 --> 00:34:01,570
+negative تمام وفي عندي كمان ال Gram Negative
+
+352
+00:34:01,570 --> 00:34:08,950
+Bacilli بتضم الاستيريا مونو سايتو جين يبقى ال Gram
+
+353
+00:34:08,950 --> 00:34:12,790
+Positive Bacilli في عندي أنا بعمل فحص الكتلاز نفس
+
+354
+00:34:12,790 --> 00:34:13,310
+الفكرة
+
+355
+00:34:43,130 --> 00:34:46,030
+يبقى الـ gram positive بصل لاي بتضم البصلص
+
+356
+00:34:46,030 --> 00:34:50,230
+Clostridium والاستيريا مونو سايتوجين عشان افرق
+
+357
+00:34:50,230 --> 00:34:55,110
+بينهم بعمل فحص الكتلاز لو طلعت كتلاز positive يبقى
+
+358
+00:34:55,110 --> 00:34:58,530
+في عندي احتمال اما انها تكون بصلص او الاستيريا
+
+359
+00:34:58,530 --> 00:35:03,010
+مونو سايتوجين لو كانت negative يبقى هي Clostridium
+
+360
+00:35:03,010 --> 00:35:07,350
+يبقى هي بتكون بصلص عندي او Clostridium او
+
+361
+00:35:07,350 --> 00:35:11,280
+الاستيريا مونو سايتوجين بعمل فحص الكتلازلو طلعت
+
+362
+00:35:11,280 --> 00:35:28,860
+positive أو طلعت negative لو كانت negative لو
+
+363
+00:35:28,860 --> 00:35:31,620
+كانت negative يبقى هي a closed medium
+
+364
+00:36:06,780 --> 00:36:12,780
+لكن لو كانت positive فى عندى احتمالين اما
+
+365
+00:36:12,780 --> 00:36:19,400
+انها تكون bustle loss او
+
+366
+00:36:19,400 --> 00:36:25,440
+انها تكون listeria monocytogen bustle
+
+367
+00:36:25,440 --> 00:36:30,580
+loss or listeria
+
+368
+00:36:30,580 --> 00:36:35,600
+mono
+
+369
+00:36:44,510 --> 00:36:51,410
+Cytogen يبقى البصلص والستيريا مونو Cytogen كتلاز
+
+370
+00:36:51,410 --> 00:36:59,530
+positive كيف
+
+371
+00:36:59,530 --> 00:37:03,250
+نفرق بين الاستفيلو والماكرو والمايكرو هنتعرف عليها
+
+372
+00:37:03,250 --> 00:37:07,410
+في المعامل الجاي لكن بالنسبة للبصلص والستيريا مونو
+
+373
+00:37:07,410 --> 00:37:11,420
+Cytogenلو انا بدي افرق بينهم بفحص اللي هو الـ
+
+374
+00:37:11,420 --> 00:37:32,820
+Sparkstan Sparkstan Sparkstan
+
+375
+00:37:34,310 --> 00:37:38,230
+بعمله لو طلعت أسبار farming يبقى هي البصي اللاص
+
+376
+00:37:38,230 --> 00:37:42,390
+لكن لو طلعت من أسبار farming يبقى هي الاستيريا
+
+377
+00:37:42,390 --> 00:37:46,750
+مونو سايتوجين هك انا اقدرت ابدأ اتعرف على
+
+378
+00:37:46,750 --> 00:37:49,870
+البكتيريا يبقى لو عملت gram stand طلعت gram
+
+379
+00:37:49,870 --> 00:37:53,840
+positiveفي عندى احتمال استريبتو كوكاي استفيلو
+
+380
+00:37:53,840 --> 00:37:56,780
+كوكاى الماكرو كوكاى المايكرو كوكاى كيف بدي افرق
+
+381
+00:37:56,780 --> 00:37:59,700
+بينهم بعمل فحص ال catalase لو طلعت negative يبقى
+
+382
+00:37:59,700 --> 00:38:02,880
+هى استريبتو كوكاى لو طلعت positive فى عندى تلت
+
+383
+00:38:02,880 --> 00:38:06,260
+احتمالات كيف افرق بينهم وان نتعرف في المعامل اللى
+
+384
+00:38:06,260 --> 00:38:10,640
+جاي فى الفحوصات اللى هتمر معناهالو عملت جرامستين و
+
+385
+00:38:10,640 --> 00:38:14,020
+طلعت جرام positive بصل لاي في عندي الجرام positive
+
+386
+00:38:14,020 --> 00:38:17,380
+بصل لاي بتضم لكلوستريديوم البصلص الستيريا مونو
+
+387
+00:38:17,380 --> 00:38:20,900
+سايتوجين كاف دي افرق بينهم وبعمل فحص الكتلاز لو
+
+388
+00:38:20,900 --> 00:38:24,140
+طلعت نيجاتيف يبقى هي الكلوستريديوم لو طلعت
+
+389
+00:38:24,140 --> 00:38:27,980
+positive يبقى عندي احتمالان اما البصلص او الستيريا
+
+390
+00:38:27,980 --> 00:38:31,040
+مونو سايتوجين كاف دي افرق بينهم هذه المعلومة
+
+391
+00:38:31,040 --> 00:38:35,560
+أخدناها في المعامل السابقة من خلال الالباصيلاز
+
+392
+00:38:35,560 --> 00:38:39,000
+تعتبر Spar Farming لكن الاستيريا مونو سايتوجين
+
+393
+00:38:39,000 --> 00:38:44,840
+تعتبر Non Spar Farming لجرام نيجاتف كوككاي في عندي
+
+394
+00:38:44,840 --> 00:38:48,020
+النايسيريا والموريكز اللي هنتعرف عليهم برضه في
+
+395
+00:38:48,020 --> 00:38:51,160
+المعامل اللي جاي لجرام نيجاتف باصيلاي في عند ال
+
+396
+00:38:51,160 --> 00:38:55,500
+انتيرو بكترياسي و الصودوموناص هدول برضه كتلازة
+
+397
+00:38:55,500 --> 00:38:57,880
+تنتان كتلازة positive بيبقى هذا الفحص أنا
+
+398
+00:38:57,880 --> 00:39:02,220
+مابستخدمه لا تفريقهناخد فحوصات أقدر أنا أميز بين
+
+399
+00:39:02,220 --> 00:39:03,860
+الـ Gram Negative بس الـ Lie اللي هي الـ
+
+400
+00:39:03,860 --> 00:39:07,400
+Enterobacteriasi و الـ Sodomonas في المعامل الجاي
+
+401
+00:39:07,400 --> 00:39:13,320
+هيك خلصنا احنا الـ Significant لهذا الفحص هنمر على
+
+402
+00:39:13,320 --> 00:39:13,920
+الـ Slides
+
+403
+00:39:31,100 --> 00:39:34,900
+الـ Significant لهذا الفحص هيفرق لبن لـ Gram
+
+404
+00:39:34,900 --> 00:39:38,360
+Positive كوكاية قلنا لستفيلو كوكاية هي كتلاز
+
+405
+00:39:38,360 --> 00:39:42,460
+positive لكن لستربتو كوكاية كتلاز negative كمان
+
+406
+00:39:42,460 --> 00:39:46,540
+هيفرق ل Gram Positive بصلاي قلنا الباصلة is كتلاز
+
+407
+00:39:46,540 --> 00:39:50,400
+positive where Clostridium is كتلاز negative ال
+
+408
+00:39:50,400 --> 00:39:54,480
+Antirobacterias اللي هي تعتبر Gram Negative بصلاي
+
+409
+00:39:54,760 --> 00:39:57,720
+ار كتلاز بوزيتف قلنا لجرام نيجاتف بس الله عند
+
+410
+00:39:57,720 --> 00:40:01,380
+الانتيرو بكترياس يوصو ضموناس والدين تاني يعتبروا
+
+411
+00:40:01,380 --> 00:40:05,820
+كتلاز بوزيتف كيف هنفرق بينهم في عند فحص الأكسداز
+
+412
+00:40:05,820 --> 00:40:11,280
+هيمور معنا في الشباتر الجايالـ Stereomonocytogen
+
+413
+00:40:11,280 --> 00:40:14,740
+قلنا هي gram positive بصلاي وهي كتلاز positive كيف
+
+414
+00:40:14,740 --> 00:40:20,040
+بدي افرق بينها وبين البصلص قلنا في عين دي فحص اللي
+
+415
+00:40:20,040 --> 00:40:23,840
+هو فحص الـ spar stand هي non spar farming لكن
+
+416
+00:40:23,840 --> 00:40:30,480
+البصلص spar farming الفحص بيفرق بين ال .. أي فحص
+
+417
+00:40:30,480 --> 00:40:34,540
+هيفرقلي بين اللي بيكونوا بنفس المجموعةيعني ما بقدر
+
+418
+00:40:34,540 --> 00:40:38,720
+أحكي عن الـ Catalase يستخدم للتفريق بين الـ Closed
+
+419
+00:40:38,720 --> 00:40:41,700
+Medium و الـ Staff لأن الـ Closed Medium بالاصل هي
+
+420
+00:40:41,700 --> 00:40:44,880
+إجرام Positive بص الله و الـ Staff إجرام Positive
+
+421
+00:40:44,880 --> 00:40:49,980
+كوكاية يعني بالاصل أنا بقدر أني أنا أفصل بينهم
+
+422
+00:40:49,980 --> 00:40:54,540
+الفحص نفسه هو بيفرق بين اللي بيكونوا بنفس المجموعة
+
+423
+00:40:54,540 --> 00:40:57,880
+يعني لو كانوا كلهم إجرام Positive كوكاية أو إجرام
+
+424
+00:40:57,880 --> 00:41:02,760
+Positive بص الله و هكذاهيك خلصنا الـ Significant
+
+425
+00:41:02,760 --> 00:41:05,760
+للفحص كيف طريقة الشغل
+
+426
+00:41:09,860 --> 00:41:14,480
+How to do the test فى عندي طريقتين عشان أشتغل هذا
+
+427
+00:41:14,480 --> 00:41:18,100
+الفحص الطريقة الأولى اللى هى slide method فى عندي
+
+428
+00:41:18,100 --> 00:41:21,740
+طريقة تانية لتيوب method ال slide method بقولى
+
+429
+00:41:21,740 --> 00:41:27,380
+بجيب أنا slide بحط drop of 3% hydrogen peroxide
+
+430
+00:41:27,380 --> 00:41:31,840
+اللى هو ال substrate ال H2O2 كيف بدأ أحضر 3%
+
+431
+00:41:31,840 --> 00:41:35,700
+hydrogen peroxide قولنا بنضيف 3 ml من ال H2O2
+
+432
+00:41:35,700 --> 00:41:41,100
+موجود كيميائى هو محضر كيميائىبنضيف عليه ديستيل
+
+433
+00:41:41,100 --> 00:41:48,360
+واتر 97 مل من ديستيل واتر بنضيف هذا الرياجينت على
+
+434
+00:41:48,360 --> 00:41:52,920
+جلوس صافية بعدين باخد Loop of test organism باخد
+
+435
+00:41:52,920 --> 00:41:56,780
+كولونيه لبكتيريا اللى انا بدي اكشف هل موجود
+
+436
+00:41:56,780 --> 00:42:02,300
+الانزيم عندها او لأ بعمل mix بعدين بلاحظ النتيجة
+
+437
+00:42:03,000 --> 00:42:06,560
+الـ tube method طريقة الـ tube بقولّي أنه أنا بجيب
+
+438
+00:42:06,560 --> 00:42:11,020
+بزرع البكتيريا اللي بدي أكشف عنها بجراء بأجار
+
+439
+00:42:11,020 --> 00:42:21,800
+إسلانت يبقى بدي أزرع بأجار إسلانت بجيب
+
+440
+00:42:21,800 --> 00:42:26,920
+طبعا media nutrient أجار مزروعة بالإسلانت بتكون
+
+441
+00:42:26,920 --> 00:42:30,700
+إسلانت بشكل مائل كيف بنزرع الأجار الإسلانت قلنا
+
+442
+00:42:30,700 --> 00:42:34,690
+بندخلبالجزء الأسفل بنعمل طعنة وبعدين بنطلع بنعمل
+
+443
+00:42:34,690 --> 00:42:38,110
+زيكزاك بعد بأزرع البكتيريا اللى بدي أكشف عنها
+
+444
+00:42:38,110 --> 00:42:42,570
+بعملها incubation في ال incubator لمدة 24 ساعة بعد
+
+445
+00:42:42,570 --> 00:42:47,690
+24 ساعة بلاحظ نمو البكتيريا بقوللي بتضيفي 1 مل من
+
+446
+00:42:47,690 --> 00:42:52,390
+ال reagent اللى هو 3% hydrogen peroxide على اللى
+
+447
+00:42:52,390 --> 00:42:57,610
+هو على سطح الإسلان تمام بعد هبشوف النتيجة كيف ال
+
+448
+00:42:57,610 --> 00:43:00,950
+result بتكون ال result هنشوفها في ال slide اللى
+
+449
+00:43:00,950 --> 00:43:07,270
+بعدالنتيجة لو كانت النتيجة positive هذا بالنسبة لل
+
+450
+00:43:07,270 --> 00:43:11,890
+slide method سيشوف النتيجة ال positive هتكون على
+
+451
+00:43:11,890 --> 00:43:15,570
+شكل air bubble يبقى لو اتكون عندي air bubble يبقى
+
+452
+00:43:15,570 --> 00:43:19,270
+هي positive كتلازملكن لو ما اتكون اشي يبقى هي
+
+453
+00:43:19,270 --> 00:43:22,630
+negative catalase او بنحكي smooth suspension بيكون
+
+454
+00:43:22,630 --> 00:43:25,490
+ال suspension smooth مافي عندي اي اشي مافي عندي
+
+455
+00:43:25,490 --> 00:43:29,830
+air bubble هذا بالنسبة ل slide method ل tube
+
+456
+00:43:29,830 --> 00:43:34,210
+method نفس الفكرة بعد ما اضيف الواحد مل من ال
+
+457
+00:43:34,210 --> 00:43:37,470
+hydrogen peroxide بقولي بتكون air bubbles في ال
+
+458
+00:43:37,470 --> 00:43:40,130
+tube زي ما احنا ملاحظين يبقى هي high positive
+
+459
+00:43:40,130 --> 00:43:44,210
+catalase لكن هان ماما نلاحظ في عندي اي فقعات يبقى
+
+460
+00:43:44,210 --> 00:43:49,580
+هينفس الفكرة للسلايد ميثود نفس الفكرة فى اندي air
+
+461
+00:43:49,580 --> 00:43:53,200
+bubbles يبقى كتلاس بوزيتف لكن هن فى اندي smooth
+
+462
+00:43:53,200 --> 00:43:59,720
+suspension يبقى هن نيجاتف للكتلس هيك اللى هى ال
+
+463
+00:43:59,720 --> 00:44:00,100
+result
+
+464
+00:44:19,840 --> 00:44:24,560
+هذه الـ slide هنتكلم عن بعض الملاحظات الهمة بقول
+
+465
+00:44:24,560 --> 00:44:27,860
+لي be careful when using bacteria from blood agar
+
+466
+00:44:27,860 --> 00:44:30,860
+culture and avoid touching the agar by loop
+
+467
+00:44:30,860 --> 00:44:34,780
+because blood cells in agar also had catalase
+
+468
+00:44:34,780 --> 00:44:39,160
+enzyme بيعطيني falls positive بقول لي ممنوع أخد
+
+469
+00:44:39,160 --> 00:44:42,620
+كولونيه عشان أعمل فحص اللي هو ال catalase test من
+
+470
+00:44:42,620 --> 00:44:47,540
+blood agarقلنا البلد أجار هي Enriched Media منضيف
+
+471
+00:44:47,540 --> 00:44:52,240
+البلد اللى هو supplement يعتبر بنسبة 5% يعني لو
+
+472
+00:44:52,240 --> 00:44:56,400
+أنا حضرت 1 liter من ال nutrient أجار بضيف 50 ml من
+
+473
+00:44:56,400 --> 00:44:58,900
+البلد طبعا بعد ما أعاقم ال media و تطلع من ال
+
+474
+00:44:58,900 --> 00:45:02,920
+autoclave بستنها تبرد بعدين بضيف البلد عشان ما
+
+475
+00:45:02,920 --> 00:45:07,880
+يتكسر طيب ليش أنا ممنوع أخد كولوني من البلد أجار
+
+476
+00:45:08,150 --> 00:45:11,930
+بقولّي لو كنت زارعة بكتيريا على إبلاد أجار وعملتي
+
+477
+00:45:11,930 --> 00:45:15,970
+فحص الـ Catalase ممكن يعطيني false positive result
+
+478
+00:45:15,970 --> 00:45:20,830
+ليش؟ لأنك لما تاخدي كولوني من إبلاد أجار ممكن تروح
+
+479
+00:45:20,830 --> 00:45:25,350
+تاخدي مع الـ colony تجرح الأجار يعني تاخدي شوية
+
+480
+00:45:25,350 --> 00:45:30,690
+media يعني blood قولنا لـ blood cells نفسها بتحتوي
+
+481
+00:45:30,690 --> 00:45:35,610
+على Catalase enzyme بالتاليممكن البكتيريا ما يكون
+
+482
+00:45:35,610 --> 00:45:39,450
+عندها أنزيم الـ Catalase لكن انت أخدت كله عن طريق
+
+483
+00:45:39,450 --> 00:45:43,470
+أو من طبق ال blood agar و جرحت هذا ال agar يعني
+
+484
+00:45:43,470 --> 00:45:47,050
+أخدت blood اللي موجود فيه Catalase enzyme بالتالي
+
+485
+00:45:47,050 --> 00:45:51,910
+هياتفاعل مع ال substrate اللي احنا حاطينها ال H2O2
+
+486
+00:45:51,910 --> 00:45:56,710
+وبعطيني positive resultعشان هيك او بيعطيني false
+
+487
+00:45:56,710 --> 00:46:00,550
+positive result نتيجة خاطئة مش البكتيريا عندها ال
+
+488
+00:46:00,550 --> 00:46:03,670
+enzyme عشان هيك الأفضل اني انا اشتغل اي فحص
+
+489
+00:46:03,670 --> 00:46:07,550
+كيميائي على general media على nutrient agar لو
+
+490
+00:46:07,550 --> 00:46:11,830
+اضطريت اني انا ااخد من blood agar بحاول اني انا
+
+491
+00:46:11,830 --> 00:46:16,010
+ااخد بدون ما اجرح ال media avoid touching the agar
+
+492
+00:46:16,010 --> 00:46:20,520
+by the loopهذا بالنسبة للملاحظة الأولى الملاحظة
+
+493
+00:46:20,520 --> 00:46:23,480
+التانية also don't use bacteria from old culture
+
+494
+00:46:23,480 --> 00:46:27,320
+because the enzyme activity drop by time بيعطيني
+
+495
+00:46:27,320 --> 00:46:31,160
+false negative اي فحص كيميائي لازم اني انا اشتغله
+
+496
+00:46:31,160 --> 00:46:35,360
+تكون ال culture fresh لأنه لو كانت old culture
+
+497
+00:46:35,360 --> 00:46:39,060
+بتكون البكتيريا منهجة تمام ال enzyme activity
+
+498
+00:46:39,060 --> 00:46:44,770
+بيبدأ EL فبالتالي بيعطيني false negative resultآخر
+
+499
+00:46:44,770 --> 00:46:48,490
+ملاحظة احنا هنضيفها و احنا نحكيها قبل ما نختم بالـ
+
+500
+00:46:48,490 --> 00:46:52,750
+Catalase Test هلأ مش كل البكتيريا قلنا عندها
+
+501
+00:46:52,750 --> 00:46:54,990
+Enzyme الـ Catalase على سبيل المثال قلنا الـ
+
+502
+00:46:54,990 --> 00:46:58,770
+Streptococci ما عندها Enzyme الـ Catalase طب قلنا
+
+503
+00:46:58,770 --> 00:47:02,910
+خلال التنفس الخلوي بيتم إنتاج الـ H2O2 اللي هو
+
+504
+00:47:02,910 --> 00:47:07,690
+يعتبر toxic worst product كيف البكتيريا اللي ما
+
+505
+00:47:07,690 --> 00:47:11,570
+عندها Enzyme الـ Catalase هتتخلص من الـ H2O2 اللي
+
+506
+00:47:11,570 --> 00:47:14,680
+يعتبر toxicوقلنا ان البكتيريا اللى معاها عندها
+
+507
+00:47:14,680 --> 00:47:18,300
+انزيم الـ catalase عندها انزيم آخر بيقوم بنفس
+
+508
+00:47:18,300 --> 00:47:23,240
+الوظيفة اللى هو اسمه hydrogen peroxidase من اسمه
+
+509
+00:47:23,240 --> 00:47:25,740
+hydrogen peroxidase يعني بيكسر ال hydrogen
+
+510
+00:47:25,740 --> 00:47:29,580
+peroxide هيشتغل على نصف ال substrate اللى هى ال
+
+511
+00:47:29,580 --> 00:47:35,780
+H2O2 لكن هيعطينى جزيئان ميه هيعطينى two جزيئان
+
+512
+00:47:35,780 --> 00:47:40,780
+H2O2النواتج هتختلف هيعطيني جزيئان ميا مش هيعطيني
+
+513
+00:47:40,780 --> 00:47:45,320
+O2 فبالتالي مش هقدر انا استدل على التفاعل هذا زي
+
+514
+00:47:45,320 --> 00:47:51,260
+ال Catalase Test هذا بالنسبة لفحص Catalase Test
+
+515
+00:47:51,260 --> 00:47:54,520
+خلصناه هنتقل ل ال Coagulase Test
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ZJy0jbwSDlU_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ZJy0jbwSDlU_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..4b764809fb1a12001a0f00f2ff340b39c2d649f6
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ZJy0jbwSDlU_postprocess.srt
@@ -0,0 +1,2048 @@
+1
+00:00:03,050 --> 00:00:06,790
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:06,790 --> 00:00:10,390
+جميعا في هذا اللقاء هنستكمل ال biochemical test
+
+3
+00:00:10,390 --> 00:00:15,550
+هنتكلم عن اليورياز اندوال ان نيترات reduction test
+
+4
+00:00:15,550 --> 00:00:21,090
+هنبدأ باليورياز test من اسمه يورياز test يبقى ال
+
+5
+00:00:21,090 --> 00:00:26,050
+enzyme اللي هكشف عنه في هذا الفحص اليورياز يعتبر
+
+6
+00:00:26,050 --> 00:00:30,850
+اندوانزايم هيشتغل على اليوريا as substrate يبقى ال
+
+7
+00:00:30,850 --> 00:00:36,870
+substrateفي هذا الفحص اليوريا انزيم اليورياز هيكسر
+
+8
+00:00:36,870 --> 00:00:40,570
+اليوريا اليوريا قلنا عبارة عن toxic product
+
+9
+00:00:40,570 --> 00:00:45,350
+اليورياز هيعمل breakdown ل اليوريا هنتخلص من
+
+10
+00:00:45,350 --> 00:00:50,410
+اليوريا عن طريق انزيم اليورياز اللى بتفرزه بعض
+
+11
+00:00:50,410 --> 00:00:55,950
+أنواع البكتيريا بنفس الوقت اليوريا تعتبر non
+
+12
+00:00:55,950 --> 00:01:01,560
+carbohydrate carbon sourceالكربوهيدريات هو المصدر
+
+13
+00:01:01,560 --> 00:01:06,640
+الأول لحصول على ال carbon source اللازم لنمو
+
+14
+00:01:06,640 --> 00:01:11,460
+البكتيريا في حال ما كان عندي كربوهيدريات أو كان
+
+15
+00:01:11,460 --> 00:01:15,320
+موجود وخلص الكربوهيدريات البكتيريا هتلجأ لمصادر
+
+16
+00:01:15,320 --> 00:01:20,560
+أخرى حتى تحصل على ال carbon source اللازم لنمو
+
+17
+00:01:20,560 --> 00:01:25,340
+البكتيريامن هذه المصادر السيترات اللى اتكلمنا عنه
+
+18
+00:01:25,340 --> 00:01:29,900
+في المحاضرة السابقة وعرفنا ان بعض أنواع البكتيريا
+
+19
+00:01:29,900 --> 00:01:33,740
+عندها القدرة على استهلاك السيترات عندها انزيمات
+
+20
+00:01:33,740 --> 00:01:38,320
+بتكسر السيترات بالنهاية بحصل على ال bio fat اللى
+
+21
+00:01:38,320 --> 00:01:44,620
+يعتبر كcarbon source كمصدر للكربون اللازم لنمو
+
+22
+00:01:44,620 --> 00:01:48,380
+البكتيريا برضه اليوريا تعتبر non carbohydrate
+
+23
+00:01:48,380 --> 00:01:52,540
+carbon source اليوريا إذا هيتكسر اليوريا هحصل على
+
+24
+00:01:52,540 --> 00:01:57,460
+أمانيا الأمانيا تعتبر ك nitrogen source زائد CO2
+
+25
+00:01:57,460 --> 00:02:02,600
+يعتبر ك carbon source لازم لنمو البكتيريا طيب أي
+
+26
+00:02:02,600 --> 00:02:08,700
+تفاعل بيصير عشان أستدل عليهبستدل من خلال ال
+
+27
+00:02:08,700 --> 00:02:14,540
+product نواتج التفاعل فإما بضيف reagent هذا ال
+
+28
+00:02:14,540 --> 00:02:19,840
+reagent بيرتبط مع أحدى نواتج التفاعل وبيعطيني لون
+
+29
+00:02:19,840 --> 00:02:25,340
+هك بعرف أنه التفاعل صار أو ال product بيكون عبارة
+
+30
+00:02:25,340 --> 00:02:30,940
+عن مركب حمضي أو قاعدي فبالتالي بيتغير ال pHفبضيف
+
+31
+00:02:30,940 --> 00:02:35,740
+BH Indicator في الميديا عشان يدلل على حدوث التفاعل
+
+32
+00:02:35,740 --> 00:02:41,260
+في هذا الفحص عندي alkaline product الـNH3 الأمونيا
+
+33
+00:02:41,260 --> 00:02:46,080
+فبالتالي هضيف BH Indicator في الميديا عشان أعرف هل
+
+34
+00:02:46,080 --> 00:02:50,080
+صار التفاعل ولا لأ هل في عندي أنزيم اليورياز ولا
+
+35
+00:02:50,080 --> 00:02:57,050
+لأ بالنسبة لـ Procedureهستخدم يوريا أجار ال يوريا
+
+36
+00:02:57,050 --> 00:03:03,950
+أجار بصبها ب إسلانت أو ممكن أنها تكون ال يوريا
+
+37
+00:03:03,950 --> 00:03:08,590
+إبرث أو ممكن تكون أجار بلات يبقى فحص ال يوريا ممكن
+
+38
+00:03:08,590 --> 00:03:12,290
+يكون إسلانت أو أجار بلات أو إبرث لكن ال most
+
+39
+00:03:12,290 --> 00:03:17,840
+common بكون إسلانتبعقم اليوريا أجار في ال
+
+40
+00:03:17,840 --> 00:03:21,960
+filtration وليس في ال autoclave لأن اليوريا بتتكسر
+
+41
+00:03:21,960 --> 00:03:26,160
+في الحرارة فبيتم تعقيم اليوريا أجار في ال
+
+42
+00:03:26,160 --> 00:03:30,320
+filtration مكونات ال media كأي media في عندي
+
+43
+00:03:30,320 --> 00:03:34,040
+normal saline ال normal saline عشان يحافظ على ال
+
+44
+00:03:34,040 --> 00:03:37,900
+osmotic pressure في عندي mono potassium phosphate
+
+45
+00:03:37,900 --> 00:03:43,040
+هذا يعتبر buffer BH هيحافظ ليه على التغير في ال BH
+
+46
+00:03:43,910 --> 00:03:48,730
+البيبتون يعتبر بروتين كمصدر لـ nitrogen source و
+
+47
+00:03:48,730 --> 00:03:53,210
+carbon source لازم لنمو البكتيريا لكن البيبتون
+
+48
+00:03:53,210 --> 00:03:57,510
+الموجود في ميديا اليوريا أجار موجود بكمية قليلة 1
+
+49
+00:03:57,510 --> 00:04:03,010
+gram per liter في عندي اليوريا اليوريا هي ال
+
+50
+00:04:03,010 --> 00:04:06,990
+substrate اللي هيشتغل عليها انزيم اليورياز موجودة
+
+51
+00:04:06,990 --> 00:04:11,980
+بكمية كبيرة 20 gram per literالـ Urea هي الـ
+
+52
+00:04:11,980 --> 00:04:15,160
+Substrate اللي يشغل عليها الانزيم هو بالتالي هتكون
+
+53
+00:04:15,160 --> 00:04:20,740
+الـ CO2 اللي يعتبر ك carbon source لازم لنمو
+
+54
+00:04:20,740 --> 00:04:25,000
+البكتيريا الـ Dextrose اللي هو الجليكوز يعتبر ك
+
+55
+00:04:25,000 --> 00:04:29,200
+energy source كمصدر للطاقة and carbon source برضه
+
+56
+00:04:29,200 --> 00:04:35,360
+موجود بكمية قليلة حتى يعتبر carbon source لازم
+
+57
+00:04:35,360 --> 00:04:39,840
+لنمو البكتيرياأكيد بما أنه ناتج التفاول في عندي
+
+58
+00:04:39,840 --> 00:04:43,960
+alkaline product فهضيف pH indicator عشان أكشف على
+
+59
+00:04:43,960 --> 00:04:49,300
+النواتج ال pH indicator هو ال phenol red ال phenol
+
+60
+00:04:49,300 --> 00:04:53,160
+red مر معنى قبل كده في فحص ال fermentation يبقى
+
+61
+00:04:53,160 --> 00:04:58,840
+عشان نحفظ فحص ال fermentation و فحص اليوريا كان ال
+
+62
+00:04:58,840 --> 00:05:02,560
+pH الموجود ال pH indicator الموجود في ال media هو
+
+63
+00:05:02,560 --> 00:05:08,710
+phenol redالفيه non-red في الacidic بيكون لونه
+
+64
+00:05:08,710 --> 00:05:12,370
+yellow في ال alkaline بيكون لونه red في ال neutral
+
+65
+00:05:12,370 --> 00:05:16,950
+بيكون لونه orange هزرع البكتيريا اللى بدي اكشف
+
+66
+00:05:16,950 --> 00:05:21,390
+عندها انزيم الورياز ولا لأ كيف بدي انا اعمل او كيف
+
+67
+00:05:21,390 --> 00:05:25,330
+بدي ازرع ال slant بعمل stabbing للمنطقة اللى تحت
+
+68
+00:05:25,330 --> 00:05:31,570
+بعدين هطلع زيك زاج طبعا هاقم الفوهة قبل و بعد بعد
+
+69
+00:05:31,570 --> 00:05:37,090
+هعمل incubation لمدة 24 ساعةبعد ال incubation هشوف
+
+70
+00:05:37,090 --> 00:05:42,710
+اللي هي ال result النتيجة بقول لي إذا لاحظت في
+
+71
+00:05:42,710 --> 00:05:46,090
+عندي growth with a change in color of media from
+
+72
+00:05:46,090 --> 00:05:49,530
+pink to red هيكون في عندي نمولة البكتيريا إشهر
+
+73
+00:05:49,530 --> 00:05:53,410
+طبيعي في عندي بيبتون كمصدر لل نيتروجين وال carbon
+
+74
+00:05:53,410 --> 00:05:56,790
+source بكمية قليلة في عندي ديكستراز كمصدر لل
+
+75
+00:05:56,790 --> 00:06:01,180
+carbon source بكمية قليلة خلصالـ Dextrose و
+
+76
+00:06:01,180 --> 00:06:05,200
+البيبتون لأنه موجود في كمية قليلة هتلجأ لمصادر
+
+77
+00:06:05,200 --> 00:06:09,060
+تانية اللي هي ال non carbohydrate carbon source
+
+78
+00:06:09,060 --> 00:06:15,380
+اليوريا لأن عندها أنزيم اليورياز طبعا فهتكسر
+
+79
+00:06:15,380 --> 00:06:21,040
+اليوريا و هحصل على CO2 زائد NH3 عندي carbon source
+
+80
+00:06:21,040 --> 00:06:25,280
+و nitrogen source فبالتالي هتنمو البكتيرياهيتغير
+
+81
+00:06:25,280 --> 00:06:28,340
+لون ال media للون pink to red لون ال media الأصلي
+
+82
+00:06:28,340 --> 00:06:33,080
+بيكون لونه pink لكن لون pink فاتح هيتحول لون ال
+
+83
+00:06:33,080 --> 00:06:38,720
+media للون فوشي للون زهري غامق كتير فبالتالي هذه
+
+84
+00:06:38,720 --> 00:06:41,840
+النتيجة ال positive طيب ليش اتغير لون ال media
+
+85
+00:06:41,840 --> 00:06:46,520
+للون ال pink أو ال red اللون الفوشي الغامق لأنه
+
+86
+00:06:46,520 --> 00:06:50,800
+عندي أنزيم اليورياز كسر اليوريا نتج أمانيا
+
+87
+00:06:50,800 --> 00:06:55,140
+الأمانيا alkaline productالـ alkaline product طبعا
+
+88
+00:06:55,140 --> 00:07:00,340
+عندي أنا pH indicator في نول red في حال كانت في
+
+89
+00:07:00,340 --> 00:07:05,340
+حال اللي هو ال basic هيتغير اللون ال pH indicator
+
+90
+00:07:05,340 --> 00:07:09,960
+لللون الأحمر نتيجة اللون الأحمر اللي هو تكون ال
+
+91
+00:07:09,960 --> 00:07:13,720
+alkaline product الأمانية هيرفعلي ال pH وهيتحول
+
+92
+00:07:13,720 --> 00:07:19,380
+لون ال pH indicator لللون ال redمن الأمثلة على ال
+
+93
+00:07:19,380 --> 00:07:22,080
+positive النتيجة ال positive يورياز positive
+
+94
+00:07:22,080 --> 00:07:26,220
+البروتياز الهيليكو باكتر كليبسيلا نيومانيا انتيرو
+
+95
+00:07:26,220 --> 00:07:30,600
+باكتر ويرسينيا انتيرو كوليتكا بالنسبة للنتيجة ال
+
+96
+00:07:30,600 --> 00:07:36,280
+negative بقولي
+
+97
+00:07:36,280 --> 00:07:40,860
+أن في بكون عندي growth في بكون عندي نمو للبكتيريا
+
+98
+00:07:41,330 --> 00:07:44,670
+إذا بتتذكروا بفحص السيطريات كانت النتيجة النيجاتيف
+
+99
+00:07:44,670 --> 00:07:49,830
+كان عندي اللون الأخضر هو اللون ال media الأصلي قبل
+
+100
+00:07:49,830 --> 00:07:54,510
+ما أنا أزرع uninculated بيكون لونه أخضر و بيكون في
+
+101
+00:07:54,510 --> 00:07:58,430
+عندي نوع growth مش موجود في عندي نمولة البكتيريا
+
+102
+00:07:58,430 --> 00:08:02,550
+لكن في فحص اليوريا النتيجة النيجاتف بيكون في عندي
+
+103
+00:08:02,550 --> 00:08:06,350
+growth في بيكون عندي نمولة البكتيريا طيب ليش
+
+104
+00:08:06,350 --> 00:08:10,470
+هنقولنا ان النتيجة النيجاتف في عندي growthفي فحص
+
+105
+00:08:10,470 --> 00:08:15,370
+السيطرات إذا بتتذكروا ما كان في عندي مصدر آخر للـ
+
+106
+00:08:15,370 --> 00:08:18,710
+carbon غير السيطرات فبالتالي البكتيريا اللي عندها
+
+107
+00:08:18,710 --> 00:08:21,690
+القدرة على استهلاك السيطرات فقط هي اللي هتنمو كان
+
+108
+00:08:21,690 --> 00:08:26,190
+مصدر وحيد للـ carbon اللي هو السيطرات لكن في فحص
+
+109
+00:08:26,190 --> 00:08:29,650
+اليوريا في ميديا اليوريا أجار في عندي بيبتان في
+
+110
+00:08:29,650 --> 00:08:33,130
+عندي دكستراز كلها مصادر لالـ carbon و الـ nitrogen
+
+111
+00:08:33,130 --> 00:08:37,050
+لازمة لنمو البكتيريا فبالتالي حتى لو البكتيريا ما
+
+112
+00:08:37,050 --> 00:08:41,040
+عندها القدرة انهاتكسر اليوريا عندى بيبتون
+
+113
+00:08:41,040 --> 00:08:44,880
+وديكستراز موجود بكمية قليلة هتقدر انها تنمو
+
+114
+00:08:44,880 --> 00:08:49,220
+البكتيريا هذا بالنسبة ليش انه النتيجة ال negative
+
+115
+00:08:49,220 --> 00:08:52,900
+فيه بيكون فيه إجراث with not change in color of
+
+116
+00:08:52,900 --> 00:08:57,580
+media بيكون لون ال media قولنا pink لون زهري فاتح
+
+117
+00:08:57,580 --> 00:09:02,300
+او بيقول ممكن يتحول لون ال media لللون ال yellow
+
+118
+00:09:02,300 --> 00:09:06,060
+ليش بتحول اللون لللون ال yellow لأنه بيقولنا ممكن
+
+119
+00:09:06,060 --> 00:09:11,530
+البكتيرياتعمل fermentation للـ Dextrose يتكون أسد
+
+120
+00:09:11,530 --> 00:09:16,930
+ويتحول لون الـ pH لللون الأصفر يبقى ممكن يكون عنده
+
+121
+00:09:16,930 --> 00:09:20,350
+الـ negative لونه ياله نتيجة اللي هو ال
+
+122
+00:09:20,350 --> 00:09:23,810
+fermentation للـ Dextrose الموجود في ال media
+
+123
+00:09:24,200 --> 00:09:28,520
+هيتكون acidic product فبالتالي هيقلل ال BH ويتحول
+
+124
+00:09:28,520 --> 00:09:33,480
+ال BH Indicator لللون الأصفر من الأمثلة على ال
+
+125
+00:09:33,480 --> 00:09:38,980
+Ureas Negative السلمونيلا والشيجيلا يعتبروا Ureas
+
+126
+00:09:38,980 --> 00:09:43,200
+Negative بالنسبة
+
+127
+00:09:43,200 --> 00:09:50,810
+لهذا المكتوب بعمل incubation لمدة 24 ساعةtwo four
+
+128
+00:09:50,810 --> 00:09:55,490
+days بقول لي مابقدر أكتب النتيجة negative إلا بعد
+
+129
+00:09:55,490 --> 00:10:00,490
+أربع أيام من ال incubation يبقى بطلع ال tube بعد
+
+130
+00:10:00,490 --> 00:10:05,590
+24 ساعة إذا طلعت negative برجحها على ال incubator
+
+131
+00:10:05,590 --> 00:10:10,430
+بعد أربع أيام إذا ضلت negative خلاص بسجل النتيجة
+
+132
+00:10:10,430 --> 00:10:14,970
+negative لكن مابسجل النتيجة negative قبل أربع أيام
+
+133
+00:10:14,970 --> 00:10:19,600
+ليش؟ why؟ هذا السؤال مهم جداواللي في عندي بكتيريا
+
+134
+00:10:19,600 --> 00:10:24,800
+delayed urease positive بتاخد وقت أطول اكتر من 48
+
+135
+00:10:24,800 --> 00:10:30,560
+ساعة عشان تكسر ال urea وتنتج ال ammonia وترفع ال
+
+136
+00:10:30,560 --> 00:10:36,180
+pH ويتحول لون الوسط للون ال pink عشان هيك بخليها ل
+
+137
+00:10:36,180 --> 00:10:41,700
+4 ايام في المقابل في عندي rapid urease positive
+
+138
+00:10:41,700 --> 00:10:45,980
+هذه البكتيريا خلال 6 ساعات يعني قبل ال 24 ساعة
+
+139
+00:10:45,980 --> 00:10:50,820
+بتحول لونالميديا لللون الفوشى زى البروتياس
+
+140
+00:10:50,820 --> 00:10:54,800
+والهيليكوباكتر هدول يعتبروا rapid urease positive
+
+141
+00:10:54,800 --> 00:10:58,700
+حتى أنا بلاحظ خلال الست ساعات الأولى انه اتحول لون
+
+142
+00:10:58,700 --> 00:11:05,060
+ال slant لللون الفوشى لكن ال بالنسبة للبط اللى
+
+143
+00:11:05,060 --> 00:11:10,320
+تحتها ده بيكون لونه أصفر بعد 24 ساعة كل تيوب بيصير
+
+144
+00:11:10,320 --> 00:11:15,310
+لونها فوشىهذه بالنسبة ليش ان انا مكتوبة هنا بخليها
+
+145
+00:11:15,310 --> 00:11:20,270
+ل 4 أيام لان قلنا في عندي delayed urease positive
+
+146
+00:11:20,270 --> 00:11:24,670
+في نقطة تالتة مهمة بالنسبة لفحص ال urease test
+
+147
+00:11:24,670 --> 00:11:30,430
+بقول لي في عندي في ال media بيبتون قلنا ان بعض
+
+148
+00:11:30,430 --> 00:11:34,710
+أنواع البكتيريا بتعمل deamination للبيبتون مش يعني
+
+149
+00:11:34,710 --> 00:11:39,890
+deamination للبيبتون يعني هتنزع ال ammonia من
+
+150
+00:11:39,890 --> 00:11:44,120
+البيبتون من البروتينوهيرفع بما انه انتزعت ال
+
+151
+00:11:44,120 --> 00:11:47,020
+ammonia طلعت ال ammonia هيرتفع ال pH ال ammonia
+
+152
+00:11:47,020 --> 00:11:51,980
+اللي خلانه product هيتحول اللون رفعت ال pH صار
+
+153
+00:11:51,980 --> 00:11:56,780
+basic هيتحول اللون ال media لللون الأحمر او ال
+
+154
+00:11:56,780 --> 00:12:00,780
+deep pink طب السؤال هان كيف بدي افرق ان اللون
+
+155
+00:12:00,780 --> 00:12:04,320
+الأحمر كان نتيجة تكسير اليوريا ولا ال بيبطان
+
+156
+00:12:04,320 --> 00:12:11,490
+بقوللي انه التفسير لهذا الموضوعإن كمية البيبتون
+
+157
+00:12:11,490 --> 00:12:15,650
+الموجودة في الميديا قلنا 1 gram per liter لكن
+
+158
+00:12:15,650 --> 00:12:20,590
+اليوريا كانت 20 gram per liter يبقى كمية البيبتون
+
+159
+00:12:20,590 --> 00:12:25,130
+بالنسبة لليوريا كثير قليلة في عندي أنا كمان buffer
+
+160
+00:12:25,130 --> 00:12:30,030
+في الميديا mono potassium phosphate هذا ال buffer
+
+161
+00:12:30,030 --> 00:12:33,910
+هيحافظلي على التغير في البيئة لو البكتيريا عملت
+
+162
+00:12:33,910 --> 00:12:37,050
+deamination للبيبتون اللي كميته قليلة بالنسبة
+
+163
+00:12:37,050 --> 00:12:41,780
+لليورياالـ buffer هيقدر يعادل هذا التغير في الـ pH
+
+164
+00:12:41,780 --> 00:12:47,540
+لكن لو كانت نتيجة تكسير اليوريا اليوريا الموجودة
+
+165
+00:12:47,540 --> 00:12:52,800
+في ال media كتيرة فبالتالي ما بيقدر ال buffer يعمل
+
+166
+00:12:52,800 --> 00:12:57,820
+معادلة لإلها فبكون فقط اللون الأحمر تكون من تكسير
+
+167
+00:12:57,820 --> 00:13:04,980
+اليوريا هذا بالنسبة لـأهم الأشياء اللي في فحص
+
+168
+00:13:04,980 --> 00:13:09,640
+اليورياز بشكل ملخص ممكن احنا قولنا اني انا ازرع
+
+169
+00:13:09,640 --> 00:13:14,900
+بسلانت او بروث او اجار ابلات نيجي نمور على ال
+
+170
+00:13:14,900 --> 00:13:19,920
+slides يورياز
+
+171
+00:13:19,920 --> 00:13:23,040
+test some bacteria are able to produce an enzyme
+
+172
+00:13:23,040 --> 00:13:25,700
+called urease بقولي بعض أنواع البكتيريا عندها
+
+173
+00:13:25,700 --> 00:13:29,510
+القدرة انها تفرز انزيم اسمه اليوريازالذي يهاجم
+
+174
+00:13:29,510 --> 00:13:33,270
+نيتروجين والكربون في مجموعات عميقية مثل اليوريا
+
+175
+00:13:33,270 --> 00:13:34,690
+يعمل تجارب تجارب تجارب تجارب تجارب تجارب تجارب
+
+176
+00:13:34,690 --> 00:13:35,730
+تجارب تجارب تجارب تجارب تجارب تجارب تجارب تجارب
+
+177
+00:13:35,730 --> 00:13:36,270
+تجارب تجارب تجارب تجارب تجارب تجارب تجارب تجارب
+
+178
+00:13:36,270 --> 00:13:36,670
+تجارب تجارب تجارب تجارب تجارب تجارب تجارب تجارب
+
+179
+00:13:36,670 --> 00:13:56,310
+تجارب تجارب تجارب تجارب تجارب تجارب تجارب تجارب ت
+
+180
+00:13:56,630 --> 00:13:59,050
+helping to differentiate salmonella and shigella
+
+181
+00:13:59,050 --> 00:14:03,130
+species which are urease negative from the urease
+
+182
+00:14:03,130 --> 00:14:05,610
+positive non-battaging protease and some
+
+183
+00:14:05,610 --> 00:14:08,790
+citrobacter species and some homophilous species
+
+184
+00:14:08,790 --> 00:14:15,070
+are urease positive هذا الفقرة كلها ال significant
+
+185
+00:14:15,070 --> 00:14:20,970
+لفحص ال urease شو ال significant لفحص ال urease
+
+186
+00:14:20,970 --> 00:14:26,290
+فحص ال urease بقولليانه بستخدمه عشان افرق ان ما ال
+
+187
+00:14:26,290 --> 00:14:28,910
+lactose fermenter قولنا عائلة ال
+
+188
+00:14:28,910 --> 00:14:32,710
+enterobacteriaceae اللى هى تعتبر إجرام نيجاتيف
+
+189
+00:14:32,710 --> 00:14:40,530
+بصلية اكسيداز نيجاتيف بنميها على المقنكى اصده هان
+
+190
+00:14:40,530 --> 00:14:46,260
+ال differential media اللى هى المقنكىالمقنكي تعتبر
+
+191
+00:14:46,260 --> 00:14:49,480
+differential media هو selective media selective
+
+192
+00:14:49,480 --> 00:14:52,520
+media لأنه بتنمي فقط لجرام negative بكتيريا
+
+193
+00:14:52,520 --> 00:14:56,020
+مابتنمي لجرام positive بكتيريا differential media
+
+194
+00:14:56,020 --> 00:15:01,840
+لأنه بتميز اللكتاز عن ال non-lactose fermenter فلو
+
+195
+00:15:01,840 --> 00:15:05,880
+نمت البكتيريا اللكتاز fermenter بتعطيها اللون ال
+
+196
+00:15:05,880 --> 00:15:10,000
+pink لكن لو نمت البكتيريا ال non-lactose fermenter
+
+197
+00:15:10,000 --> 00:15:15,900
+بتعطيها اللون ال colorless هلأ بقولليفي هذه الفقرة
+
+198
+00:15:15,900 --> 00:15:22,360
+لو أنا وصلتني عينة شتول بروح
+
+199
+00:15:22,360 --> 00:15:25,740
+بزراها على الـ differential media المكونكي احنا
+
+200
+00:15:25,740 --> 00:15:31,160
+قلنا الـ microbiologist بختار نوع ال media حسب نوع
+
+201
+00:15:31,160 --> 00:15:36,980
+العينة من الميديات اللي انا بزراها في حال وصلتني
+
+202
+00:15:36,980 --> 00:15:44,460
+عينة شتول مكونكي بزرع عينة الشتول على مكونكيبعد ما
+
+203
+00:15:44,460 --> 00:15:48,260
+انا زراعة عينة الستول على مقنقي طلعت النتيجة non
+
+204
+00:15:48,260 --> 00:15:52,100
+-lactose fermenter كان لون ال كولوني لونها
+
+205
+00:15:52,100 --> 00:15:56,240
+colorless بقوللي في عندي تلت احتمالات ال non
+
+206
+00:15:56,240 --> 00:15:59,640
+-lactose fermenter بتتكون من مجموعة من البكتيريا
+
+207
+00:15:59,640 --> 00:16:03,680
+لكن في الستول في بكون عندي تلت احتمالات اما انها
+
+208
+00:16:03,680 --> 00:16:08,980
+تكون Salmonella, Shigellaالسلمونيلا والشيجيلا
+
+209
+00:16:08,980 --> 00:16:13,060
+يعتبروا pathogen in stool البروتياس يعتبر non
+
+210
+00:16:13,060 --> 00:16:16,680
+pathogen كيف بدي أفرق بين هدولة التلاتة بقولي
+
+211
+00:16:16,680 --> 00:16:22,500
+بتعملي فحص الورياز الورياز هيميز ال non lactose
+
+212
+00:16:22,500 --> 00:16:27,360
+fermenter في ال stool specimen على طبق أو على
+
+213
+00:16:27,360 --> 00:16:32,800
+ميديا الماكنكي لو طلعت ورياز negative يبقى هي
+
+214
+00:16:32,800 --> 00:16:36,920
+سلمونيلا وشيجيلالكن هي مكتوب salmonella and
+
+215
+00:16:36,920 --> 00:16:41,360
+shigella species which are urease negative لكن ال
+
+216
+00:16:41,360 --> 00:16:45,440
+urease positive اللى تعتبر non pathogen في ال
+
+217
+00:16:45,440 --> 00:16:49,080
+stool specimen اللى هي تعتبر ال protease ال
+
+218
+00:16:49,080 --> 00:16:52,800
+protease تعتبر urease positive يبقى بعمل فحص ال
+
+219
+00:16:52,800 --> 00:16:57,140
+urease لو طلعت positive فهي protease تعتبر non
+
+220
+00:16:57,140 --> 00:17:01,320
+pathogen في ال stool specimen لكن لو طلعت negative
+
+221
+00:17:01,320 --> 00:17:06,350
+يبقى هي salmonella او shigella هنكتب اللى فيهورياز
+
+222
+00:17:06,350 --> 00:17:10,110
+بوزيتف غير البروتياس some citrobacter species and
+
+223
+00:17:10,110 --> 00:17:14,770
+some hemophilus species يعتبروا برضه يورياز بوزيتف
+
+224
+00:17:14,770 --> 00:17:18,810
+بروتياس ميرابيلس is a major cause of human urinary
+
+225
+00:17:18,810 --> 00:17:22,910
+tract infection هان احنا قلنا انه البروتياس تعتبر
+
+226
+00:17:22,910 --> 00:17:27,870
+non pathogen in stool لكن هان بقوللي هي pathogen
+
+227
+00:17:27,870 --> 00:17:31,430
+في ال urine يبقى البروتياس ميرابيلس هي ال major
+
+228
+00:17:31,430 --> 00:17:36,390
+cause of human urinary tract infectionعشان هيك لو
+
+229
+00:17:36,390 --> 00:17:42,670
+مريض بيه الـ UTI كان وكان سبب الـ UTI بروتياس
+
+230
+00:17:42,670 --> 00:17:49,090
+بنلاحظ أنه رائحة الـ urine بتكون في رائحة اللي هي
+
+231
+00:17:49,090 --> 00:17:53,890
+رائحة الأمانيا نتيجة تكسير الـ urea بواسطة أنزيم
+
+232
+00:17:53,890 --> 00:17:57,590
+الـ ureas لأن البروتياس ureas positive فهيكسر الـ
+
+233
+00:17:57,590 --> 00:18:02,230
+urea هيطلع أمانيا الرائحة اللي هي رائحة الأمانيا
+
+234
+00:18:02,630 --> 00:18:07,490
+هذا بالنسبة لـ Significant لفحص اليورياز عشان يفرق
+
+235
+00:18:07,490 --> 00:18:10,150
+ال salmonella و الشجلة اللي يعتبر يورياز negative
+
+236
+00:18:10,680 --> 00:18:15,640
+باثوجين في الستول عن اليورياز positive اللى هى ال
+
+237
+00:18:15,640 --> 00:18:19,040
+non pathogen اللى هى البروتياز طب كيف دي افرق
+
+238
+00:18:19,040 --> 00:18:23,180
+السلمونيلا و الشيجيلا عن بعض لو طلعت يورياز
+
+239
+00:18:23,180 --> 00:18:27,620
+negative بقول في فحص ال motility و فحص ال H2S
+
+240
+00:18:27,620 --> 00:18:31,760
+السلمونيلا positive للفحسين motile and H2S
+
+241
+00:18:31,760 --> 00:18:36,420
+positive الشيجيلا non motile and H2S negative
+
+242
+00:18:36,950 --> 00:18:41,850
+البروتياس نفس السلمونيلا موتايل زائد H2S positive
+
+243
+00:18:41,850 --> 00:18:45,890
+طبعا بفرق بينهم بفحص اللي هو اليورياز البروتياس
+
+244
+00:18:45,890 --> 00:18:50,690
+positive والسلمونيلا negative لليورياز هذا بالنسبة
+
+245
+00:18:50,690 --> 00:18:58,750
+ل ال significant للفحص اليورياز
+
+246
+00:18:58,750 --> 00:19:02,070
+producing by some enterobacteriasemia اليورياز
+
+247
+00:19:02,070 --> 00:19:04,150
+positive قولنا البروتياس لكلبسلال
+
+248
+00:19:04,150 --> 00:19:08,260
+enterobacterialiersinia enterocoliticaالهيليكوبكتر
+
+249
+00:19:08,260 --> 00:19:12,520
+بيولوري هو أكسداز بوزيتف قلنا الهيليكوبكتر زي ال
+
+250
+00:19:12,520 --> 00:19:19,380
+.. زي ال vibrio cholera زي ال aeromonas هدال كانوا
+
+251
+00:19:19,380 --> 00:19:27,000
+أكسداز بوزيتف لكن كانت ال Sodomonas أكسداز زيهم
+
+252
+00:19:27,000 --> 00:19:31,960
+Sodomonas أكسداز بوزيتف الهيليكوبكتر ال aeromonas
+
+253
+00:19:31,960 --> 00:19:36,640
+ال vibrio لكن الإختلاف بينها وبين Sodomonasإنه الـ
+
+254
+00:19:36,640 --> 00:19:40,500
+Helicobacter بقول لي هي Gelicose Fermenter لكن
+
+255
+00:19:40,500 --> 00:19:45,520
+الصدومونس كانت Non-Gelicose Fermenter الـ
+
+256
+00:19:45,520 --> 00:19:49,220
+Helicobacter قلنا إنه هي Urease Positive بكتيرية
+
+257
+00:19:49,220 --> 00:19:53,100
+تعتبر Rapid Urease Positive خلال ست ساعات بتتحول
+
+258
+00:19:53,100 --> 00:19:57,180
+اللون للون بنكية و البروتياس الـ Helicobacter و
+
+259
+00:19:57,180 --> 00:20:00,400
+البروتياس يعتبرهم Rapid Urease Positive
+
+260
+00:20:05,110 --> 00:20:07,530
+الـ Principle عشان اني انا افرق اليوريا is
+
+261
+00:20:07,530 --> 00:20:10,150
+positive و اليوريا is negative بستخدم اللي هي
+
+262
+00:20:10,150 --> 00:20:14,190
+اليوريا agar قولنا بتحتوي على يوريا بنسبة 20 جرام
+
+263
+00:20:14,190 --> 00:20:20,750
+per liter per liter جيلاتين بيبتون هدول مصادر
+
+264
+00:20:20,750 --> 00:20:24,750
+يعتبر ال protein مصادر ال nitrogen و ال carbon
+
+265
+00:20:24,750 --> 00:20:28,250
+source موجود بكمية قليلة صوديوم الكلورايت قولنا
+
+266
+00:20:28,250 --> 00:20:31,770
+عشان يحافظ على ال ozone pressure ديكستروز موجود
+
+267
+00:20:31,770 --> 00:20:36,460
+بكمية قليلة قولنا كمصدر للكربونمصدر للطاقة في non
+
+268
+00:20:36,460 --> 00:20:40,620
+-red هو الـ pH Indicator مونوبوتاسيومفوسفات قلنا
+
+269
+00:20:40,620 --> 00:20:44,100
+اللي هو ال buffer اللي هيحفظلي على التغير في الـ
+
+270
+00:20:44,100 --> 00:20:47,700
+pH some bacteria can utilize urea as non
+
+271
+00:20:47,700 --> 00:20:50,480
+-carbohydrate carbon source قلنا الـ urea تعتبر
+
+272
+00:20:50,480 --> 00:20:53,940
+non-carbohydrate carbon source باستخدام أنزيم الـ
+
+273
+00:20:53,940 --> 00:20:59,420
+urease هي أنزيم الـ urease وهي الـ urea هي الرابط
+
+274
+00:20:59,420 --> 00:21:03,410
+ما بين الكربون والنيتروجينمابين الكربون
+
+275
+00:21:03,410 --> 00:21:07,190
+والنيتروجين هتيجي اليورياز ويكسر هاي الرابطة
+
+276
+00:21:07,190 --> 00:21:12,630
+ويتكون عندي أمونيا زائد CO2 يورياز activity the
+
+277
+00:21:12,630 --> 00:21:15,890
+urea is detected by growing bacteria in media
+
+278
+00:21:15,890 --> 00:21:20,430
+containing urea يوريا هتكون كsubstrate وبستخدم pH
+
+279
+00:21:20,430 --> 00:21:25,130
+indicator اللي هو ال phenol red لو اليوريا اتكسرت
+
+280
+00:21:25,130 --> 00:21:29,690
+بواسطة انزيم اليورياز الامونيا هتكون ناتج من نواتج
+
+281
+00:21:29,690 --> 00:21:36,530
+عمليةالتكسير فبالتالي هيعملي الوسط alkaline هيرفع
+
+282
+00:21:36,530 --> 00:21:40,810
+ال pH فبالتالي ال indicator هيتحول لونه للون
+
+283
+00:21:40,810 --> 00:21:47,150
+الأحمر to deep pink او انه هيتحول للون زي ال
+
+284
+00:21:47,150 --> 00:21:51,750
+violet هذا هو ال positive لفحص اليوريا Dextrose
+
+285
+00:21:51,750 --> 00:21:54,790
+قلنا موجود بكمية قليلة في ال media فبالتالي
+
+286
+00:21:54,790 --> 00:22:01,130
+البكتيريا هتروح تلجأ لمصدر تاني ل carbon sourceأو
+
+287
+00:22:01,130 --> 00:22:05,290
+انها تتوقف على النمو في حال ما كان في عندها انزيم
+
+288
+00:22:05,290 --> 00:22:10,870
+اليورياز اكيد هتتوقف على النمو لأن البيبتون و
+
+289
+00:22:10,870 --> 00:22:14,630
+الديكستراز اللي يعتبروا كمصدر الكاربون اللازم لنمو
+
+290
+00:22:14,630 --> 00:22:19,410
+البكتيريا خلص موجود بكمية قليلة هتلجأ لا اليورياز
+
+291
+00:22:19,410 --> 00:22:23,470
+في حال ما كان عندها يورياز اكيد هتتوقف على النمو
+
+292
+00:22:23,470 --> 00:22:27,790
+في اي حال عندها يورياز البكتيريا كانت يورياز
+
+293
+00:22:27,790 --> 00:22:31,930
+positive اكيد هتلجأ لامصدر اليوريا عشان تحصل على
+
+294
+00:22:31,930 --> 00:22:36,790
+carbon source نلاحظ
+
+295
+00:22:36,790 --> 00:22:40,610
+في الصورة أنه في non-red في البيئة في الاسدك بيكون
+
+296
+00:22:40,610 --> 00:22:45,270
+لونه yellow لكن في ال basic بيكون لونه فوشي هيك
+
+297
+00:22:45,270 --> 00:22:49,090
+بيكون النتيجة ال positive هذا اللون الفوشي في حال
+
+298
+00:22:49,090 --> 00:22:50,630
+كان عندي يورياز positive
+
+299
+00:22:53,640 --> 00:22:58,600
+الطريقة أني أنا بزرع ال slant في ال media اليوريا
+
+300
+00:22:58,600 --> 00:23:02,400
+بال test organism بال organism اللي بدي أكشف عنه
+
+301
+00:23:02,400 --> 00:23:07,600
+هعمل incubation لمدة 24 ساعة ل 4 days و عرفنا ليش
+
+302
+00:23:07,600 --> 00:23:13,040
+أنا بدي أخلي اليوتيوب لمدة 4 أياملأن في عندي delay
+
+303
+00:23:13,040 --> 00:23:19,560
+urease positive بكتيريا النتيجة بشوفها بعد 48 ساعة
+
+304
+00:23:19,560 --> 00:23:22,840
+هي some bacteria have a delay urease reaction that
+
+305
+00:23:22,840 --> 00:23:27,180
+may require an incubation period longer than أكتر
+
+306
+00:23:27,180 --> 00:23:31,760
+من 48 ساعة النتيجة ال positive بشوف لون bright
+
+307
+00:23:31,760 --> 00:23:35,320
+pink color هيتطور فيه الإسلان بعدين ممكن أنه يمتد
+
+308
+00:23:35,320 --> 00:23:39,070
+في ال media خلال أول تلت ساعات بشوفه بالإسلانفلما
+
+309
+00:23:39,070 --> 00:23:43,150
+اتكمل ال 24 ساعة بشوفه ممتد في كل ال media في ال
+
+310
+00:23:43,150 --> 00:23:46,730
+negative لا تغير في ال original color of media مش
+
+311
+00:23:46,730 --> 00:23:49,510
+هلاحظ اي تغير في لون ال media او قولنا هيتحول
+
+312
+00:23:49,510 --> 00:23:56,550
+اللون للون الأصفر هذه نتيجة ال positive و ال
+
+313
+00:23:56,550 --> 00:23:59,370
+negative هان احنا قولنا ممكن تكون breath ممكن تكون
+
+314
+00:23:59,370 --> 00:24:02,510
+slant هذه breath النتيجة ال negative ما تغير لون
+
+315
+00:24:02,510 --> 00:24:05,310
+ال media النتيجة ال positive كان في عندي اللون
+
+316
+00:24:05,310 --> 00:24:09,240
+فوشيhigh slant النتيجة ال positive اللون فوشي في
+
+317
+00:24:09,240 --> 00:24:13,560
+كل تيوب لكن ال negative ما تغير لون ال media ال
+
+318
+00:24:13,560 --> 00:24:16,860
+rabbit positive قلنا خلال أول ست ساعات بلاحظ أن
+
+319
+00:24:16,860 --> 00:24:21,840
+اللون الفوشي كان في ال slant لكن لو أنا خلاتها أو
+
+320
+00:24:21,840 --> 00:24:26,760
+كملت ل 24 ساعة هلاحظ أنه هيمتد اللون الفوشي في كل
+
+321
+00:24:26,760 --> 00:24:30,920
+تيوب high negative هي ما تغير لون ال media دلوا
+
+322
+00:24:30,920 --> 00:24:35,300
+اللون اللي ما أخد no change on color of the media
+
+323
+00:24:35,730 --> 00:24:39,390
+هذا بالنسبة لفحص اليورياز
+
+324
+00:24:47,090 --> 00:24:51,570
+هنيجي هلأ للفحص التاني اللي هو ال Indole Test ال
+
+325
+00:24:51,570 --> 00:24:55,430
+Indole Test هيكشف ليه على أنزيم لتريبتوفاناز
+
+326
+00:24:55,430 --> 00:25:00,550
+لتريبتوفاناز يعتبر اندو انزايم من اسمه هيشتغل على
+
+327
+00:25:00,550 --> 00:25:05,050
+لتريبتوفان as substrate لتريبتوفان يعتبر amino
+
+328
+00:25:05,050 --> 00:25:09,790
+acid ال amino acid لتريبتوفاناز هيكسر هذا ال amino
+
+329
+00:25:09,790 --> 00:25:13,950
+acid عشان أحصل على carbon source و nitrogen source
+
+330
+00:25:13,950 --> 00:25:20,150
+لازم لنمو البكتيرياالتريبتوفان من مكوناته اندول و
+
+331
+00:25:20,150 --> 00:25:24,010
+بيروفات و أمونيا هدولة تلاتة لكن بيكونوا متصلين
+
+332
+00:25:24,010 --> 00:25:28,990
+ببعض مع بعض مرتبطين في حال البكتيريا أفرت ذات
+
+333
+00:25:28,990 --> 00:25:33,230
+إنزيم للتريبتوفانات هتكسر التريبتوفان اللي هو ال
+
+334
+00:25:33,230 --> 00:25:38,810
+substrate هتعطيني اندول زائد بيروفات زائد أمونيا
+
+335
+00:25:39,840 --> 00:25:43,400
+هدولة التلاتة هتعطيهم بشكل منفصل كانوا هم مع بعض
+
+336
+00:25:43,400 --> 00:25:49,040
+مرتبطين ببعض ب .. لتريبتوفان باسم لتريبتوفان
+
+337
+00:25:49,040 --> 00:25:51,640
+لتريبتوفان عبارة عن اندول و بيروفيت و أمونيا
+
+338
+00:25:51,640 --> 00:25:55,660
+مرتبطين ببعض إجا أنزيم لتريبتوفانياز كسر
+
+339
+00:25:55,660 --> 00:25:59,880
+لتريبتوفان و أعطاني اندول زائد بيروفيت زائد أمونيا
+
+340
+00:25:59,880 --> 00:26:03,780
+طبعا الأمونيا تستخدم ك نيتروجين سورس البيروفيت
+
+341
+00:26:03,780 --> 00:26:09,150
+تستخدم لمصدد التقوي ال carbon sourceهذا بالنسبة
+
+342
+00:26:09,150 --> 00:26:13,750
+للفحص ال principle كيف انا بدي اشتغل هذا الفحص
+
+343
+00:26:13,750 --> 00:26:17,790
+بقوللي بجيب media بتحتوي على ال substrate ال
+
+344
+00:26:17,790 --> 00:26:22,410
+triptofan ممكن استخدم media اسمها ال triptone
+
+345
+00:26:22,410 --> 00:26:26,370
+broth بتكون ال broth بتحتوي على ال triptofan ال
+
+346
+00:26:26,370 --> 00:26:31,850
+substrate او ممكن استخدم media اسمها sim ال sim
+
+347
+00:26:31,850 --> 00:26:38,150
+بتكون هاي ال media slant مش ال broth ال simأو
+
+348
+00:26:38,150 --> 00:26:44,670
+sorry بتكون stab مش slant انعدل هذه الكلمة ال S I
+
+349
+00:26:44,670 --> 00:26:48,150
+M بتكون stab زي اللي استخدمناها في فحص ال motility
+
+350
+00:26:48,150 --> 00:26:54,610
+ال S بترمز أو بتكشف على فحص ال H2S ال I بتكشف على
+
+351
+00:26:54,610 --> 00:26:59,670
+فحص ال indol و ال M بتكشف على فحص ال motility هذه
+
+352
+00:26:59,670 --> 00:27:03,190
+ال media تعتبر single media multiple test هنتعرف
+
+353
+00:27:03,190 --> 00:27:07,290
+في المحاضرة الجايإن أنا ميديا واحدة ممكن تكشفلي
+
+354
+00:27:07,290 --> 00:27:10,830
+على أكتر من فحص كيميائي فهتكشف على الـ edge to
+
+355
+00:27:10,830 --> 00:27:13,790
+edge وهتكشف على ال motility وهتكشف على ال indoor
+
+356
+00:27:13,790 --> 00:27:18,450
+فممكن أستخدم هذه الميديا أو ممكن أستخدم ميديا
+
+357
+00:27:18,450 --> 00:27:22,370
+لتريبتون إبروث اللي فقط هتكشفلي على ال indoor لكن
+
+358
+00:27:22,370 --> 00:27:26,330
+هذه الميديا هتكشفلي على تلت فحوصات من ضمنهم ال
+
+359
+00:27:26,330 --> 00:27:31,510
+indoor لو استخدمت لتريبتون إبروث هاخد البكتيريا
+
+360
+00:27:31,510 --> 00:27:36,290
+اللي بدي أكشف هل انتالنزيم للتريبتوفاناس وهعمل mix
+
+361
+00:27:36,290 --> 00:27:42,590
+في البروث لو كانت SIM هي أستاذ فهعمل stabbing طعنة
+
+362
+00:27:42,590 --> 00:27:48,470
+في المنتصف و هشوف النتيجة بعد 24 ساعة هعمل
+
+363
+00:27:48,470 --> 00:27:53,910
+incubation لمدة 24 ساعةبقولنا انه عشان اكشف عن
+
+364
+00:27:53,910 --> 00:27:59,070
+التفاعل انه صار ولا لأ بدي اضيف reagent هذا ال
+
+365
+00:27:59,070 --> 00:28:03,210
+reagent هيرتبط مع احد لو هتنجح التفاعل هيرتبط مع
+
+366
+00:28:03,210 --> 00:28:08,570
+واحد من هدول هيعطيني لون هيك بعرف ان التفاعل صار
+
+367
+00:28:08,570 --> 00:28:12,970
+صار ولا لأ بقوللي في فحص ال indulge test بعد ال
+
+368
+00:28:12,970 --> 00:28:18,290
+incubation بتضيفي covax reagent ال covax reagent
+
+369
+00:28:18,290 --> 00:28:23,200
+بقوللييرتبط او من مكونات الـ COVAX REAGENT بالأول
+
+370
+00:28:23,200 --> 00:28:29,500
+خلّيني نحكي ISOAMILE ALCOHOL HCL وفيه مادة اللي هي
+
+371
+00:28:29,500 --> 00:28:36,700
+ال BMAB هدولة التلت مكونات من مكونات الـ COVAX
+
+372
+00:28:36,700 --> 00:28:40,800
+REAGENT هضيف الـ COVAX REAGENT بعد 24 ساعة
+
+373
+00:28:40,800 --> 00:28:45,080
+incubation هعمل شكلة لتيوب و هقرأ النتيجة
+
+374
+00:28:45,080 --> 00:28:50,050
+immediatelyبقول لي لو اتكون حلقة لونها cherry red
+
+375
+00:28:50,050 --> 00:28:55,150
+ring زي لون الكرز يبقى هي positive للفحص
+
+376
+00:28:55,150 --> 00:28:58,970
+الاندواليندها انزيم لـ tryptophanase مثال عليها
+
+377
+00:28:58,970 --> 00:29:05,150
+الـ E.coli لو صار ما لو ما صار عندي اي تغيير ما
+
+378
+00:29:05,150 --> 00:29:09,470
+اتكون حلقة لونها زي لون الكرز يبقى هي negative زي
+
+379
+00:29:09,470 --> 00:29:13,510
+ال E.clipsilla و ال Enterobacter طيب كيف صار
+
+380
+00:29:13,510 --> 00:29:18,030
+التفاعل او كيف اتكون الحلقة الحمراءقلنا إن الـ
+
+381
+00:29:18,030 --> 00:29:22,050
+Covax reagent اللي أنا أضفته برتبط مع أحد نواتج
+
+382
+00:29:22,050 --> 00:29:26,210
+التفاعل و اللي ال Covax reagent برتبط مع ال Indol
+
+383
+00:29:26,210 --> 00:29:34,890
+مين بالتحديد اللي بيرتبط مع ال Indol من مكونات ال
+
+384
+00:29:34,890 --> 00:29:38,990
+Covax reagentمن مكونات الـ Govex Reagent اللى
+
+385
+00:29:38,990 --> 00:29:43,810
+بيرتبط مع الاندالي اللى هو ال بي ام ابي هدى
+
+386
+00:29:43,810 --> 00:29:49,090
+هيرتبطوا تنين هدول مع بعض تمام ويكونولى Complex
+
+387
+00:29:49,090 --> 00:29:53,670
+هذا ال Complex هيرتبط مع بعضترسّب في القاع تمام في
+
+388
+00:29:53,670 --> 00:29:59,090
+قاع اليوتيوب هيترسّب هييجي ال HCL هيرفع ال complex
+
+389
+00:29:59,090 --> 00:30:03,070
+و يتكون حلقة في أعلى اليوتيوب يبقى كذا فالتفاعل
+
+390
+00:30:03,070 --> 00:30:06,970
+بيصير ال triptofan يزكسر ال triptofan الموجود في
+
+391
+00:30:06,970 --> 00:30:10,990
+ال media بعد ال incubation هضيف ال covax reagent
+
+392
+00:30:10,990 --> 00:30:17,490
+ال covax reagent أحد مكوناته ال BMABهذه هترتبط مع
+
+393
+00:30:17,490 --> 00:30:22,170
+نواتج التفاعل اللى هو بالتحديد الاندول هيكوّن ليه
+
+394
+00:30:22,170 --> 00:30:26,990
+Complex هيترسّب في قاع ال tube هيجي ال HCl اللى
+
+395
+00:30:26,990 --> 00:30:31,370
+يعتبر مكوّن من مكونات ال covax reagent هيرفع ال
+
+396
+00:30:31,370 --> 00:30:37,430
+complex ويكوّن اللى هو الحلقة الحمراء بقولي ال HCl
+
+397
+00:30:37,430 --> 00:30:41,290
+لو كان لو أنا استخدمت تربتون البروثممكن إن أنا
+
+398
+00:30:41,290 --> 00:30:46,110
+أستغني عنه من ما أضيفه على الـ Covax Reagent لكن
+
+399
+00:30:46,110 --> 00:30:51,810
+في حال كانت ال media SIM اللي هي Stabbing بتكون
+
+400
+00:30:51,810 --> 00:30:55,910
+semi solid فبالتالي صعب إن أنا أستغني عنه لأنه ما
+
+401
+00:30:55,910 --> 00:31:03,290
+بيقدر يطلع لفوق بحتاج لـ SCL هو اللي يطلعه لفوقهذا
+
+402
+00:31:03,290 --> 00:31:08,210
+بالنسبة للفحص هيو الـ N دول قلنا بيرتبط مع الـ
+
+403
+00:31:08,210 --> 00:31:12,670
+PMAB هيتوّن Complex اللي هي الـ Sherry Red هتترسب
+
+404
+00:31:12,670 --> 00:31:17,190
+في القاح هييجي الـ SCL يرفعها بقول لي في Reagent
+
+405
+00:31:17,190 --> 00:31:20,890
+تاني ممكن اني انا في حال ما في عندي Covax Reagent
+
+406
+00:31:20,890 --> 00:31:25,850
+اني انا اضيفه عشان اكشف انه التفاعل صاراسمه
+
+407
+00:31:25,850 --> 00:31:29,850
+Ehrlich-reagent من مكوناته نفس المكونات تبعت
+
+408
+00:31:29,850 --> 00:31:34,130
+الكوفكس-reagent لكن الاختلاف بدل ال Isoamyl
+
+409
+00:31:34,130 --> 00:31:38,870
+Alcohol هستخدم Ethyl Alcohol الفرق بين الكوفكس وال
+
+410
+00:31:38,870 --> 00:31:43,510
+Ehrlich-reagent أنه ال Ehrlich-reagent يعتبر more
+
+411
+00:31:43,510 --> 00:31:48,990
+sensitive يعني لو كمية قليلةموجودة في ال media الـ
+
+412
+00:31:48,990 --> 00:31:54,910
+Tryptophan هيكشف على هذه الكيمية هذا الفرق بين
+
+413
+00:31:54,910 --> 00:32:03,510
+الالخ reagent و ال Kovacs reagent طيب احنا قلنا
+
+414
+00:32:03,510 --> 00:32:08,190
+اذا بتتذكروا في فحص ال Catalase ال Catalase كان ال
+
+415
+00:32:09,270 --> 00:32:11,690
+الـ substrate اللى بيشتغل عليها الانزيم الـ
+
+416
+00:32:11,690 --> 00:32:17,150
+hydrogen peroxide قولنا في حال ما كان عندي كتالياز
+
+417
+00:32:17,150 --> 00:32:20,190
+في حال البكتيريا ما أفرزت انزيم الكتالياز ال
+
+418
+00:32:20,190 --> 00:32:25,050
+hydrogen peroxide يعتبر toxic فبالتالي البكتيريا
+
+419
+00:32:25,050 --> 00:32:28,870
+اللى كانت negative للكتالياز هتبقى هيكون انت انزيم
+
+420
+00:32:28,870 --> 00:32:31,950
+تانى عشان تتخلص من ال hydrogen peroxide اللى هو ال
+
+421
+00:32:31,950 --> 00:32:38,230
+hydrogen peroxidase قولنا برضه لـ tryptophan عشان
+
+422
+00:32:38,230 --> 00:32:41,670
+اني اناأستفيد منه وأحصل على carbon و nitrogen
+
+423
+00:32:41,670 --> 00:32:44,310
+source في حال البكتيريا ما كان فيه عندها
+
+424
+00:32:44,310 --> 00:32:48,930
+tryptophan S في بكتيريا عندها أنزيم تاني اسمه
+
+425
+00:32:48,930 --> 00:32:54,070
+tryptophan deaminase هذا هيشتغل على نفس ال
+
+426
+00:32:54,070 --> 00:32:57,910
+substrate اللي اشتغل عليها أنزيم ال tryptophan S
+
+427
+00:32:57,910 --> 00:33:02,850
+هيعمل deamination احنا قلنا ال tryptophan بتكون من
+
+428
+00:33:02,850 --> 00:33:07,310
+endol and pyruvate وNH3 مربوطين في بعضفبالتالي
+
+429
+00:33:07,310 --> 00:33:10,850
+انزيم لـ tryptophan D-aminase هيجي يعمل D
+
+430
+00:33:10,850 --> 00:33:16,750
+-amination لـ tryptophan هيرفع الأمانيا لحال هيخل
+
+431
+00:33:16,750 --> 00:33:20,090
+الاندول و البيروفات مربوطين ببعض D-aminase يبقى
+
+432
+00:33:20,090 --> 00:33:24,770
+هيعمل D-amination هيزيل الأمانيا لكن هيخل الاندول
+
+433
+00:33:24,770 --> 00:33:30,630
+و البيروفات مع بعض كيف بدي أكشف عن أنه صارت تفاعل
+
+434
+00:33:30,630 --> 00:33:35,290
+بقولنا أني أنا بدي أضيف reagentعشان يرتبط مع ناتج
+
+435
+00:33:35,290 --> 00:33:39,690
+من نواتج التفاعل في هذه الحالة بضيف reactant اللي
+
+436
+00:33:39,690 --> 00:33:45,030
+هو ال triptofan-d-aminase reactant مكيونته FeCl3
+
+437
+00:33:45,030 --> 00:33:50,150
+زي الـ HCl زي الـ distilled waterمين بالظبط اللى
+
+438
+00:33:50,150 --> 00:33:56,030
+هيرتبط بالنواتج التفاعل من مكونات الـ TDR agent
+
+439
+00:33:56,030 --> 00:34:01,210
+واللي الـ FECL3 أحد مكونات الـ tryptophan D
+
+440
+00:34:01,210 --> 00:34:06,090
+امينازر agent هيرتبط مع الاندول بيروفات اللى هو
+
+441
+00:34:06,090 --> 00:34:11,750
+ناتج من نواتج التفاعل هيعطيني complex اسمه
+
+442
+00:34:11,750 --> 00:34:16,370
+فرقهيدرازون هذا لونه brown shred يبقى النتيجة ال
+
+443
+00:34:16,370 --> 00:34:20,230
+positive هي ال brownshredلكن النتيجة الـ negative
+
+444
+00:34:20,230 --> 00:34:26,070
+لازم تتغير لون الـ media هذا بالنسبة لفحص
+
+445
+00:34:26,070 --> 00:34:31,050
+التريبتوفان D-aminase في حال البكتيريا ما عندها
+
+446
+00:34:31,050 --> 00:34:34,250
+انزيم للتريبتوفان D-aminase هتستخدم انزيم آخر حتى
+
+447
+00:34:34,250 --> 00:34:37,110
+تستفيد من التريبتوفان D-aminase حتى تحصل على
+
+448
+00:34:37,110 --> 00:34:43,070
+carbon و nitrogen source طيب، شو وظيفة الـ HCL-HAN
+
+449
+00:34:43,940 --> 00:34:51,720
+بقولنا ان الـ HCl-Han بيمنع ارتباط لتريبتوفان مع
+
+450
+00:34:51,720 --> 00:34:56,240
+الـ FECL3 هلأ لو البكتيريا ما عندها انزيم
+
+451
+00:34:56,240 --> 00:35:01,300
+لتريبتوفان دي اميناز هيضلوا لتريبتوفان موجود في ال
+
+452
+00:35:01,300 --> 00:35:07,280
+media اضفت رأي جاندي لأحد مكوناته الـ FECL3 بقول
+
+453
+00:35:07,280 --> 00:35:12,240
+لي الـ FECL3 لومقلق الاندوهال بيروفات يعني ماكان
+
+454
+00:35:12,240 --> 00:35:15,540
+انزيم لتريبتوفان دي اميناز موجود اللي هيكسر
+
+455
+00:35:15,540 --> 00:35:19,120
+لتريبتوفان هيروح يرتبط مع ال substrate مع
+
+456
+00:35:19,120 --> 00:35:23,940
+لتريبتوفان ويعطيني لون أحمر أو لون brown shred
+
+457
+00:35:23,940 --> 00:35:28,990
+فبالتالي هيعطيني false positive resultالـ HCl
+
+458
+00:35:28,990 --> 00:35:34,810
+هيمنع هذا الإرتباط فبالتالي هيمنع إنه يتكون لون
+
+459
+00:35:34,810 --> 00:35:38,990
+أحمر نتيجة إرتباط الـ FACL الـ Tree و الـ
+
+460
+00:35:38,990 --> 00:35:42,830
+Tryptophan مع بعض و يعطوني False Positive Result
+
+461
+00:35:42,830 --> 00:35:48,150
+هذا وظيفة الـ HCl في الـ TDA Reagent لكن الـ HCl
+
+462
+00:35:48,150 --> 00:35:52,970
+في الـ COVAX Reagent اللي هو يرفع الـ Complex اللي
+
+463
+00:35:52,970 --> 00:35:54,770
+اتكون في القاعلة الأعلى
+
+464
+00:35:57,990 --> 00:36:02,030
+نجي للسلايدات الاندول test the ability to degrade
+
+465
+00:36:02,030 --> 00:36:06,630
+amino acid to identifiable end product is often
+
+466
+00:36:06,630 --> 00:36:09,330
+used to differentiate among bacteria بقول لي هو
+
+467
+00:36:09,330 --> 00:36:13,450
+هذا الفحص هيكشف على قدرة البكتيريا انها تكسر ال
+
+468
+00:36:13,450 --> 00:36:16,990
+amino acid فبالتالي هيساعدني ان انا افرق بين
+
+469
+00:36:16,990 --> 00:36:20,830
+البكتيريا tryptophan for example is hydrolyzed to
+
+470
+00:36:20,830 --> 00:36:24,850
+endol pyruvic acid and ammonia قولنا من نواتج
+
+471
+00:36:24,850 --> 00:36:30,040
+التفاعل اندولبيروفات و أمونيا هيتكسروا بواسطة
+
+472
+00:36:30,040 --> 00:36:34,160
+التريبتوفاناز لأن التريبتوفان دي أميناز ان دول مع
+
+473
+00:36:34,160 --> 00:36:39,420
+البيروفات هيدلوا و الأمونيا هتكون لحال البيروفيك
+
+474
+00:36:39,420 --> 00:36:42,140
+أسد قلنا انه بيصير metabolized to produce large
+
+475
+00:36:42,140 --> 00:36:45,520
+amount of energy هتكون مصدر للطاقة الأمونيا
+
+476
+00:36:45,520 --> 00:36:50,500
+ستستخدم قلنا عشان أعمل او يتكون new amino acid
+
+477
+00:36:50,500 --> 00:36:55,670
+كمصدر للنيتروجين و الكربون اللازم لتكوين ال amino
+
+478
+00:36:55,670 --> 00:36:59,870
+acid فبالتالي هيتكون لبروتين اللازم لنمو البكتيريا
+
+479
+00:36:59,870 --> 00:37:04,730
+ال end ده اللي باكشف عنه كيف قولنا انه بضيف covax
+
+480
+00:37:04,730 --> 00:37:09,850
+reagent بيتكون من بارا diamethyl amino
+
+481
+00:37:09,850 --> 00:37:14,430
+benzaldehyde in alcohol قولنا هذا هو الإختصار ال B
+
+482
+00:37:15,620 --> 00:37:21,940
+الـ BMAB هذا هي اختصارها أحد مكونات الـ GovEx
+
+483
+00:37:21,940 --> 00:37:26,740
+Reagent تقولنا بترتبط بالاندالي اللي هو أحد نواتج
+
+484
+00:37:26,740 --> 00:37:31,340
+التفاعل هيعطيني اللي هو بالنهاية Complex هذا ال
+
+485
+00:37:31,340 --> 00:37:36,240
+Complex لونه أحمر Cherry Red Ring الـ FCL هيعمل
+
+486
+00:37:36,240 --> 00:37:41,720
+على رفع هذا الراسب لفوق في Lithium هيتكون Red
+
+487
+00:37:41,720 --> 00:37:45,990
+Colorالبرو سيجر انا هعمل incubation بيه لتريبتو
+
+488
+00:37:45,990 --> 00:37:51,010
+لتريبتون البروث RSIM ميديا المهم ميديا بتحتوي على
+
+489
+00:37:51,010 --> 00:37:55,150
+لتريبتوفان اللي هيكون ك substrate هعمل incubation
+
+490
+00:37:55,150 --> 00:37:58,810
+بعد ال incubation هضيف ال covax reagent هعمل
+
+491
+00:37:58,810 --> 00:38:02,550
+shacking و هقرأ immediately هاي الإندول اللي هو
+
+492
+00:38:02,550 --> 00:38:08,540
+ناتج من نواتش عملية تكسير لتريبتوفانهذا الـ Covax
+
+493
+00:38:08,540 --> 00:38:12,180
+Reagent قلنا مكوناته الـ B-dimethyl
+
+494
+00:38:12,180 --> 00:38:16,540
+-aminobenzaldehyde و الـ HCL و الـ aminalcohol
+
+495
+00:38:16,540 --> 00:38:21,820
+بالآخر هيتكوّن لعندي sherry red ring النتيجة قلنا
+
+496
+00:38:21,820 --> 00:38:24,860
+red ring in the top layer indicate the presence of
+
+497
+00:38:24,860 --> 00:38:29,840
+indol لكن في حال غياب اللون هذا بيدل إنه الindol
+
+498
+00:38:29,840 --> 00:38:34,960
+ما اتكون فبالتالي إنزيم لتربطه فناز مش موجود في
+
+499
+00:38:34,960 --> 00:38:39,210
+البكتيرياهذا الفحص ashes significant له و بقولنا
+
+500
+00:38:39,210 --> 00:38:44,430
+بيميز ال E coli اللي هي positive لهذا الفحص هيتكون
+
+501
+00:38:44,430 --> 00:38:49,070
+red ring في ال top from negative اللي negative
+
+502
+00:38:49,070 --> 00:38:52,430
+لهذا الفحص لكلب سلة و ال interior باكتر ما هتكون
+
+503
+00:38:52,430 --> 00:38:57,710
+اي red ring قولنا ال MVEC اللي هو ال Indol و ال
+
+504
+00:38:57,710 --> 00:39:01,270
+Methyl Red Vox Bicarb Citrate هدول الأربع فحوصات
+
+505
+00:39:01,270 --> 00:39:05,740
+يستخدموا للتفريق ال coliform عن بعضلو البكتيريا
+
+506
+00:39:05,740 --> 00:39:10,080
+طلعت lactose fermenter انتيرو بكتيريا لكتاز فرمنتر
+
+507
+00:39:10,080 --> 00:39:12,240
+عشان افرق الـ Coliform اللي هي الـ Eclipsilla
+
+508
+00:39:12,240 --> 00:39:17,160
+سيتروباكتر و الانتيروباكتر و ال E.coli بستخدم أربع
+
+509
+00:39:17,160 --> 00:39:21,640
+فحوصات اللي هم ال Endol Red Vagas Biscuit و
+
+510
+00:39:21,640 --> 00:39:27,900
+السيترات عشان أفرق الأربع الأربع هدول genus عن بعض
+
+511
+00:39:31,190 --> 00:39:36,490
+هك بنكون خلصنا ال test Indole هنتقل لاخر فحص
+
+512
+00:39:36,490 --> 00:39:39,630
+كيميائي اللي هو ال test Nitrate Reduction
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/_HOd9t_eBH4.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/_HOd9t_eBH4.srt
new file mode 100644
index 0000000000000000000000000000000000000000..e1abbeebcfe5a6f8c1a98e65194241f557bb62bc
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/_HOd9t_eBH4.srt
@@ -0,0 +1,1455 @@
+1
+00:00:01,890 --> 00:00:04,990
+من الأمثلة الأخرى على single media multiple test
+
+2
+00:00:04,990 --> 00:00:10,690
+ليسين آيرون أجار ليسين آيرون أجار، من اسمها أكيد فيه
+
+3
+00:00:10,690 --> 00:00:14,690
+الميديا فيه عندي ليسين، الليسين يعتبر amino acid في
+
+4
+00:00:14,690 --> 00:00:18,290
+الميديا،  بدي أكشف هل البكتيريا بتعمل deamination
+
+5
+00:00:18,290 --> 00:00:23,190
+لليسين أو decarboxylation لليسين، آيرون بما أنه
+
+6
+00:00:23,190 --> 00:00:26,830
+فيه آيرون في اسم الميديا، يبقى فيه عندي H2S
+
+7
+00:00:26,830 --> 00:00:31,720
+production، بدي أكشف على الـH2S، الأجار يبقى الـ
+
+8
+00:00:31,720 --> 00:00:35,440
+solidifying agent الموجود في الميديا هو الأجار
+
+9
+00:00:35,440 --> 00:00:42,120
+بنسبة 1.5%، فبالتالي هصب الميديا في slant.
+
+10
+00:00:42,120 --> 00:00:46,560
+عشان أوفر anaerobic condition من خلال slant لأنه
+
+11
+00:00:46,560 --> 00:00:49,120
+بدي أكشف في هذه الميديا على الـ deamination
+
+12
+00:00:49,120 --> 00:00:53,800
+لليسين والـ deamination للجيلاتين، وهدول بيسيروا
+
+13
+00:00:53,800 --> 00:00:59,090
+في الـ anaerobic condition، كمان بدي أنا أكشف على الـ
+
+14
+00:00:59,090 --> 00:01:02,130
+decarboxylation لليسين والـ fermentation للجليكوز
+
+15
+00:01:02,130 --> 00:01:05,350
+وهدول بيصيروا في الآن anaerobic condition اللي من
+
+16
+00:01:05,350 --> 00:01:10,030
+خلال slant بيوفر هاي الظروف في هاي الميديا فيه
+
+17
+00:01:10,030 --> 00:01:12,830
+indicator، بما أنه بكشف على الـ fermentation
+
+18
+00:01:12,830 --> 00:01:17,730
+لجليكوز فأكيد لازم، وكمان هكشف على الـ deamination
+
+19
+00:01:17,730 --> 00:01:21,310
+لليسين أو الجيلاتين، فبالتالي هيتكون acidic or
+
+20
+00:01:21,310 --> 00:01:25,920
+alkaline product، فلازم يكون في عندي pH indicator الـ
+
+21
+00:01:25,920 --> 00:01:29,500
+pH indicator الموجود في هاي الميديا أول مرة
+
+22
+00:01:29,500 --> 00:01:34,320
+يمر معنا براموثيرمول بلو، هذا الـ pH
+
+23
+00:01:34,320 --> 00:01:38,260
+indicator في الـ acidic condition بيكون لونه yellow، في
+
+24
+00:01:38,260 --> 00:01:41,320
+الـ basic condition بيكون لونه purple، في الـ neutral
+
+25
+00:01:41,320 --> 00:01:46,670
+بيكون لونه greyish أو بيكون لونه violet فاتح،  قلنا في
+
+26
+00:01:46,670 --> 00:01:50,750
+عندي في هاي الميديا، هكشف على الـ H2S production، فأكيد
+
+27
+00:01:50,750 --> 00:01:55,390
+لازم يكون H2S indicator اللي هو الـ ferric ammonium
+
+28
+00:01:55,390 --> 00:02:00,710
+citrate، تمام، هذا بالنسبة لمكونات الـ media، وعن شو
+
+29
+00:02:00,710 --> 00:02:05,310
+بتكشف الـ media، أول أشياء البكتيريا هتلجأ لإلها
+
+30
+00:02:05,310 --> 00:02:10,990
+كمصدر للطاقة و للغذاء اللي هو الجليكوز، فلو
+
+31
+00:02:10,990 --> 00:02:13,370
+البكتيريا عندها القدرة أنها تعمل fermentation
+
+32
+00:02:13,370 --> 00:02:19,170
+لجليكوز، هيتكون عندي product acidic، هيتحول لون الـ
+
+33
+00:02:19,170 --> 00:02:23,770
+media للون yellow، يبقى في حال كان في عندي جليكوز
+
+34
+00:02:23,770 --> 00:02:28,770
+fermentation positive، يبقى هيتحول لون slant بما أنه
+
+35
+00:02:28,770 --> 00:02:33,270
+صار في ظروف هوائية، هيتحول لون slant للون yellow
+
+36
+00:02:33,270 --> 00:02:37,290
+ثاني شيء، خلص الجليكوز، الجليكوز موجود في الـ media
+
+37
+00:02:37,290 --> 00:02:41,110
+بكمية قليلة، نفس triple sugar iron agar، موجود
+
+38
+00:02:41,110 --> 00:02:46,770
+بنسبة 1 من 10%، فالبكتيريا في حال أنه خلص
+
+39
+00:02:46,770 --> 00:02:51,030
+الجليكوز، أكيد هتلجأ لمصادر أخرى للحصول على الطاقة
+
+40
+00:02:51,030 --> 00:02:55,970
+و للتغذية، فبالتالي هتروح لليسين، الليسين موجود في
+
+41
+00:02:55,970 --> 00:02:58,610
+الـ media، وهي الـ media أنا بدي أكشف عن قدرة
+
+42
+00:02:58,610 --> 00:03:01,610
+البكتيريا إنها تعمل deamination لليسين أو
+
+43
+00:03:01,610 --> 00:03:06,370
+decarboxylation، أو إذا لاحظين أنه مكتوب أو ممنوع
+
+44
+00:03:06,370 --> 00:03:10,710
+أن البكتيريا يكون عندها القدرة إنها تعمل لليسين
+
+45
+00:03:10,710 --> 00:03:14,400
+deamination و decarboxylation مع بعض، إما هتعمل de-
+
+46
+00:03:14,400 --> 00:03:17,900
+amination أو de-carboxylation، أو ممكن ما تعمل أي
+
+47
+00:03:17,900 --> 00:03:20,840
+شيء لليسين، لكن الاثنين مع بعض ممنوع أني أنا
+
+48
+00:03:20,840 --> 00:03:25,360
+أشوفهم، هلأ الـ de-amination لليسين هيتكون عندي
+
+49
+00:03:25,360 --> 00:03:30,340
+alkaline product، تمام، المفترض بما أنه اتكون عندي
+
+50
+00:03:30,340 --> 00:03:34,540
+alkaline product، هيرفع الـ pH، هيتحول لون الـ media
+
+51
+00:03:34,540 --> 00:03:38,020
+للون purple، لكن إذا من اللحظة إذا بتلاحظوا مكتوب
+
+52
+00:03:38,020 --> 00:03:42,480
+هنا بتحول لون الـ media للون red، طيب ليش اتحول لون
+
+53
+00:03:42,480 --> 00:03:45,460
+الـ media للون red بالرغم أنه اتكون عندي alkaline
+
+54
+00:03:45,460 --> 00:03:50,560
+product، وفيه عندي pH indicator موجود في الـ acidic، في
+
+55
+00:03:50,560 --> 00:03:53,040
+sorry، في الـ basic condition بيكون لونه purple
+
+56
+00:03:53,040 --> 00:03:57,140
+بقول لي الـ alkaline product الناتج من عملية الـ
+
+57
+00:03:57,140 --> 00:04:02,560
+lysine deamination، قوة ارتباطه بالـ H2S indicator
+
+58
+00:04:02,560 --> 00:04:06,140
+أعلى من قوة ارتباطه بالـ pH indicator، فبالتالي
+
+59
+00:04:06,140 --> 00:04:10,130
+هيتكون اللون red، نتيجة أن الـ alkaline product
+
+60
+00:04:10,130 --> 00:04:16,530
+لـ lysine deamination، قوة ارتباطه بالـ H2S indicator
+
+61
+00:04:16,530 --> 00:04:21,690
+أعلى من الـ pH indicator، فهيتحول اللون للون red، طب
+
+62
+00:04:21,690 --> 00:04:23,830
+لو البكتيريا عندها القدرة إنها تعمل
+
+63
+00:04:23,830 --> 00:04:26,610
+decarboxylation لليسين وما عندها القدرة إنها تعمل
+
+64
+00:04:26,610 --> 00:04:31,130
+deamination لليسين، نتيجة عملية الـ decarboxylation
+
+65
+00:04:31,130 --> 00:04:35,210
+برضه هيتكون أنه عندي alkaline product، موجود عندي
+
+66
+00:04:35,210 --> 00:04:38,150
+pH indicator في الـ basic condition بيكون لونه
+
+67
+00:04:38,150 --> 00:04:42,950
+purple، فهي تحول لون الـ media للون purple، طيب لو
+
+68
+00:04:42,950 --> 00:04:46,110
+البكتيريا ما عندها القدرة إنها تعمل لليسين أي شيء
+
+69
+00:04:46,110 --> 00:04:49,370
+سواء deamination أو decarboxylation، بقول لي هيروح
+
+70
+00:04:49,370 --> 00:04:52,810
+هتروح البكتيريا لـ الجيلاتين الموجود في الـ media
+
+71
+00:04:52,810 --> 00:04:56,430
+يعتبر protein، هتعمله deamination، هيتكون عندي
+
+72
+00:04:56,430 --> 00:05:00,780
+alkaline product، موجود عندي pH indicator في الـ
+
+73
+00:05:00,780 --> 00:05:06,140
+basic condition اللي هو لونه purple، فبالتالي هيرفع
+
+74
+00:05:06,140 --> 00:05:09,360
+الـ pH، هذا الـ alkaline product هيتحول لون الـ media
+
+75
+00:05:09,360 --> 00:05:15,500
+للون purple، طيب نيجي للنتائج، هلأ الـ media فيه عندي
+
+76
+00:05:15,500 --> 00:05:18,980
+slant و butt، قلنا الـ slant بتوفر ظروف aerobic
+
+77
+00:05:18,980 --> 00:05:22,080
+condition، فالـ butt بتوفر ظروف anaerobic condition
+
+78
+00:05:22,610 --> 00:05:26,730
+هلأ بمسك اليوتيوب، بطلع على الـ slant، لو اتكون لون
+
+79
+00:05:26,730 --> 00:05:31,070
+red، يبقى بحكي إنه صار في عندي lysine deamination
+
+80
+00:05:31,070 --> 00:05:35,550
+برمز له بالرمز A، لو اتكون لون purple، بحكي صار في
+
+81
+00:05:35,550 --> 00:05:40,690
+عندي gelatin deamination، برمز له بالرمز K، لأنه
+
+82
+00:05:40,690 --> 00:05:43,370
+إحنا قلنا اللي lysine deamination والجيلاتين
+
+83
+00:05:43,370 --> 00:05:48,140
+deamination بيصيروا في ظروف aerobic condition، وقلنا
+
+84
+00:05:48,140 --> 00:05:51,760
+ناتج عملية الـ lysine deamination اللي هو الـ
+
+85
+00:05:51,760 --> 00:05:55,660
+alkaline product، قوة ارتباطه بالـ H2S indicator
+
+86
+00:05:55,660 --> 00:05:58,340
+أعلى من الـ pH indicator، فبالتالي هيتحول اللون
+
+87
+00:05:58,340 --> 00:06:01,320
+للون red، الجيلاتين deamination قلنا بتكون
+
+88
+00:06:01,320 --> 00:06:04,520
+alkaline product، بيرفع الـ pH، فبتتحول لون الـ
+
+89
+00:06:04,520 --> 00:06:08,520
+media للون purple، طيب بالنسبة للـ butt، قلنا بتوفر anaerobic
+
+90
+00:06:08,520 --> 00:06:10,580
+condition، بتكشف على الجليكوز
+
+91
+00:06:10,580 --> 00:06:13,200
+fermentation والـ lysine decarboxylation، إذا
+
+92
+00:06:13,200 --> 00:06:17,090
+اتحول لون الـ butt للون yellow، يبقى بفهم إنه
+
+93
+00:06:17,090 --> 00:06:20,270
+صار في عندي جليكوز fermentation، وبرمز له بالرمز
+
+94
+00:06:20,270 --> 00:06:24,570
+A، لو اتحول لون الـ butt لون purple، يبقى بعرف إنه صار
+
+95
+00:06:24,570 --> 00:06:28,150
+في عندي lysine decarboxylation، وبرمز له بالرمز K
+
+96
+00:06:28,150 --> 00:06:33,230
+طيب بالنسبة لـ الـ H2S production، قلنا الـ H2S
+
+97
+00:06:33,230 --> 00:06:35,670
+production بيصير في ظروف anaerobic condition
+
+98
+00:06:35,670 --> 00:06:39,660
+و بيصير في ظروف acidic condition، لو اتكون عندي الـ
+
+99
+00:06:39,660 --> 00:06:43,740
+H2S production، مش هعرف هل البكتيريا عملت glucose
+
+100
+00:06:43,740 --> 00:06:47,560
+fermentation أو lysine decarboxylation، ممكن تكون
+
+101
+00:06:47,560 --> 00:06:50,800
+عملت glucose fermentation، وممكن تكون عملت lysine
+
+102
+00:06:50,800 --> 00:06:54,100
+decarboxylation، طيب ليش ما قلنا زي media تانية
+
+103
+00:06:54,100 --> 00:06:57,840
+زي triple sugar iron agar، من حد ما أشوف الـ H2S production
+
+104
+00:06:57,840 --> 00:07:01,340
+بكتب جنبها رمز الـ A، ممنوع أني أنا أشوف رمز الـ K
+
+105
+00:07:01,340 --> 00:07:04,880
+بقول لي لأ، في هاي الـ media ممكن يكون A وممكن يكون K
+
+106
+00:07:04,880 --> 00:07:09,820
+ممكن يكون A وممكن يكون K، يبقى الـ H2S production
+
+107
+00:07:09,820 --> 00:07:13,700
+ممكن لأنها تكون عملت glucose fermentation، رمزها
+
+108
+00:07:13,700 --> 00:07:18,020
+اللي هو الـ A، ممكن يكون الـ lysine decarboxylation
+
+109
+00:07:18,020 --> 00:07:23,700
+كرمز K، هلأ البكتيريا اللي عندها القدرة لو
+
+110
+00:07:23,700 --> 00:07:27,780
+كان الرمز هلأ A ومعها H2S production، معناه أن
+
+111
+00:07:27,780 --> 00:07:31,180
+البكتيريا عملت glucose fermentation، اتكون عندي
+
+112
+00:07:31,180 --> 00:07:35,080
+acidic condition، فبالتالي صار في عندي H2S
+
+113
+00:07:35,080 --> 00:07:38,800
+production، بالنسبة للـ lysine decarboxylation هذا
+
+114
+00:07:38,800 --> 00:07:41,940
+بتكون alkaline product، وإحنا قلنا الـ H2S
+
+115
+00:07:41,940 --> 00:07:46,400
+production بيحصل في ظروف acidic condition، كيف
+
+116
+00:07:46,400 --> 00:07:49,640
+بنحكي إنه عادي ممكن أني أنا أشوف الـ H2S
+
+117
+00:07:49,640 --> 00:07:52,200
+production مع الكائنات، مع الـ lysine
+
+118
+00:07:52,200 --> 00:07:56,320
+decarboxylation؟ بقول لي قبل ما البكتيريا تعمل lysine
+
+119
+00:07:56,320 --> 00:07:59,640
+decarboxylation، بتكون في الأصل عملت glucose
+
+120
+00:07:59,640 --> 00:08:04,800
+fermentation، عملت glucose fermentation، تمام، اتكون
+
+121
+00:08:04,800 --> 00:08:07,800
+عندي acidic condition، هذا الـ acidic condition
+
+122
+00:08:07,800 --> 00:08:12,040
+اتوفر، فبالتالي البكتيريا عملت H2S production، وعملت
+
+123
+00:08:12,040 --> 00:08:14,660
+lysine decarboxylation، لأن الـ lysine
+
+124
+00:08:14,660 --> 00:08:18,180
+decarboxylation بيصير برضه نفس الـ H2S production
+
+125
+00:08:18,180 --> 00:08:22,920
+في ظروف acidic condition، الـ lysine decarboxylation
+
+126
+00:08:22,920 --> 00:08:27,880
+نفس الـ H2S production بيحصل في ظروف acidic
+
+127
+00:08:27,880 --> 00:08:32,000
+condition، فبالتالي من حد ما أشوف أن والله البكتيريا
+
+128
+00:08:32,000 --> 00:08:35,100
+صار في عندها أو عملت lysine decarboxylation، ده وهي
+
+129
+00:08:35,100 --> 00:08:37,540
+في الأصل بتكون قبلها عاملة glucose fermentation،
+
+130
+00:08:37,540 --> 00:08:41,900
+اتكون عندي acidic condition، اتحصل الـ lysine
+
+131
+00:08:41,900 --> 00:08:45,660
+decarboxylation، واتحصل اللي هو الـ H2S production
+
+132
+00:08:45,660 --> 00:08:48,340
+اللي لازم يلاقوا مع بعض، acidic condition اللي
+
+133
+00:08:48,340 --> 00:08:51,180
+ناتج من عملية الـ glucose fermentation، يبقى في هاي
+
+134
+00:08:51,180 --> 00:08:55,520
+الميديا لو عندي اتكون الـ H2S، ممكن تكون في الأصل هي
+
+135
+00:08:55,520 --> 00:08:58,680
+glucose fermentation، رمزها A، وممكن تكون رمزها K، اللي هي
+
+136
+00:08:58,680 --> 00:09:01,560
+عملت lysine decarboxylation، وقبل عملت glucose
+
+137
+00:09:01,560 --> 00:09:07,100
+fermentation، طيب مكونات الـ media، yeast extract
+
+138
+00:09:07,100 --> 00:09:10,740
+لازم لنمو البكتيريا، dextrose اللي هو موجود بكمية
+
+139
+00:09:10,740 --> 00:09:15,100
+قليلة، 1 من 10%، عشان البكتيريا تعمل fermentation، الـ
+
+140
+00:09:15,100 --> 00:09:17,560
+lysine اللي هو البكتيريا اللي هيكشف هل هتعمله
+
+141
+00:09:17,560 --> 00:09:21,160
+decarboxylation أو deamination، موجود بنسبة 1%
+
+142
+00:09:21,680 --> 00:09:24,180
+gelatin اللي هو قلنا البروتين اللي هتعمله دي-
+
+143
+00:09:24,180 --> 00:09:27,900
+amination، H2S indicator، ferric ammonium citrate
+
+144
+00:09:27,900 --> 00:09:30,720
+الـ substrates لهذا الفحص، sodium thiosulfate، الـ pH
+
+145
+00:09:30,720 --> 00:09:35,080
+indicator، bromthymol blue، الأجار كاس والـ
+
+146
+00:09:35,080 --> 00:09:38,360
+solidifying agent، الـ pH للميديا من غير ما أنا أزراعها
+
+147
+00:09:38,360 --> 00:09:42,340
+ستة أو سبعة، نلاحظ أن لون الميديا في الأصل من قبل
+
+148
+00:09:42,340 --> 00:09:48,960
+ما أنا أزراعها، بيكون لونها بنفسجي فاتح، هذه الميديا
+
+149
+00:09:48,960 --> 00:09:52,540
+بدي أكشف عن قدرة الـ Gram negative bacteria اللي
+
+150
+00:09:52,540 --> 00:09:56,800
+إنها تعمل decarboxylation أو deamination للـ lysine، هي
+
+151
+00:09:56,800 --> 00:10:00,120
+OR مش AND، يبقى البكتيريا إما هتعمل decarboxylation
+
+152
+00:10:00,120 --> 00:10:03,860
+للـ lysine أو هتعمل deamination، ممنوع الاثنين
+
+153
+00:10:03,860 --> 00:10:07,320
+يكونوا مع بعض، مش  both and،  ممكن هذا أو هذا أو
+
+154
+00:10:07,320 --> 00:10:11,560
+ممكن ما هي تعمل للثنين أي شيء، ممكن ما هي تعمل de-
+
+155
+00:10:11,560 --> 00:10:14,720
+carboxylation ولا deamination، كمان بدي أكشف عن
+
+156
+00:10:14,720 --> 00:10:18,980
+قدرتها إنها تعمل H2S production، بقول إن الليسين دي-
+
+157
+00:10:18,980 --> 00:10:21,500
+amination بيصير قلنا في الـ aerobic condition
+
+158
+00:10:21,500 --> 00:10:25,200
+فبالتالي هأكشف عنه في الـ slant، الـ decarboxylation
+
+159
+00:10:25,200 --> 00:10:28,780
+بيصير في ظروف anaerobic، فبالتالي هأكشف عنه في الـ butt
+
+160
+00:10:28,780 --> 00:10:32,140
+السالمونيلا أو الـ Citrobacter، إذا ملاحظين الاثنين
+
+161
+00:10:32,140 --> 00:10:36,140
+H2S positive، يبقى السالمونيلا اللي هيمر معنا الـ
+
+162
+00:10:36,140 --> 00:10:40,020
+Salmonella typhi H2S positive، من الكوليفورم الـ
+
+163
+00:10:40,020 --> 00:10:44,540
+Citrobacter H2S positive، إذا ملاحظ هان جنب الـ H2S
+
+164
+00:10:44,540 --> 00:10:48,370
+في الـ butt فيه  إنتاج alkaline وفيه إنتاج acidic
+
+165
+00:10:48,370 --> 00:10:51,710
+اللي هو الـ yellow، في الـ triple sugar iron agar
+
+166
+00:10:51,710 --> 00:10:55,470
+ممنوع أشوف هاي، ممنوع أشوف الـ alkaline جنب الـ H2S
+
+167
+00:10:55,470 --> 00:10:59,690
+production، لكن في الـ lysine iron agar، قلنا في حال
+
+168
+00:10:59,690 --> 00:11:03,930
+كان عندي H2S positive، ما أقدر أحدد هل هي alkaline أو
+
+169
+00:11:03,930 --> 00:11:08,230
+هل هي acidic اللي هو yellow، هل هي عملت اللي هو
+
+170
+00:11:08,230 --> 00:11:12,290
+lysine decarboxylation أو عملت glucose
+
+171
+00:11:12,290 --> 00:11:17,480
+fermentation، تمام، هذه معناها أن البكتيريا عملت
+
+172
+00:11:17,480 --> 00:11:19,960
+glucose fermentation، السالمونيلا اللي عملت glucose
+
+173
+00:11:19,960 --> 00:11:23,080
+fermentation، اتكون عندي acidic condition، الـ acidic
+
+174
+00:11:23,080 --> 00:11:26,600
+condition كان مهم جدا لـ الـ H2S production، اتكون
+
+175
+00:11:26,600 --> 00:11:31,380
+عندي الـ H2S gas، وكان مهم جدا كمان لـ الـ lysine
+
+176
+00:11:31,380 --> 00:11:36,340
+decarboxylation، فاتحول اللون بعدها للون الـ purple
+
+177
+00:11:36,340 --> 00:11:40,580
+اللي هو الـ alkaline، لكن ه
+
+223
+00:14:42,160 --> 00:14:46,940
+لون purple يبقى ما اتكون عندي اللي هو الجيلاتين
+
+224
+00:14:46,940 --> 00:14:52,260
+اللي بالنسبة للإسلاند اللون الـ red معناه اللي هو
+
+225
+00:14:52,260 --> 00:14:56,740
+Lysine deamination positive لكن ماعملت جيلاتين
+
+226
+00:14:56,740 --> 00:14:59,600
+deamination يبقى هان اللون الـ red في الإسلاند
+
+227
+00:14:59,600 --> 00:15:03,850
+معناه Lysine deamination positive يبقى Yellow الـ A
+
+228
+00:15:03,850 --> 00:15:07,470
+Red قلنا بما أن الـ Red بقى برمز لإله بالرمز R مش
+
+229
+00:15:07,470 --> 00:15:11,750
+الرمز K هاي البكتيريا فقط عندها القدرة أنها تعمل
+
+230
+00:15:11,750 --> 00:15:14,690
+Deamination للـ ليسين  عندها القدرة أنها تعمل
+
+231
+00:15:14,690 --> 00:15:18,510
+Decarboxylation للـ ليسين الـ Black precipitate
+
+232
+00:15:18,510 --> 00:15:22,710
+قلنا الـ H2S production هاي ما بقدر أشوفها أو ما
+
+233
+00:15:22,710 --> 00:15:28,010
+بقدر إذا كانت H2S production أحدد هل هي A أو هل هي
+
+234
+00:15:28,010 --> 00:15:31,700
+K هل هي Glucose fermentation أو هل هي اللي هي
+
+235
+00:15:31,700 --> 00:15:34,920
+lysine decarboxylation مع Glucose fermentation
+
+236
+00:15:34,920 --> 00:15:39,280
+لازم أعمل الفحص لحالي لازم أعمل media يكون مافيها
+
+237
+00:15:39,280 --> 00:15:44,600
+الـ substrate اللي هو للـ sodium thiosulfate
+
+238
+00:15:44,600 --> 00:15:50,880
+reductase طيب في هذه الـ media ممنوع أشوف ممنوع
+
+239
+00:15:50,880 --> 00:15:54,580
+إحنا نشوف أنه النتيجة يكون في عندي Lysine
+
+240
+00:15:54,580 --> 00:15:57,960
+deamination تمام Lysine deamination في الإسلات
+
+241
+00:15:57,960 --> 00:16:01,710
+ممنوع أشوف اللون الـ red في الـ slant واللون الـ
+
+242
+00:16:01,710 --> 00:16:06,630
+purple في الـ butt لأن اللون الـ red في الـ slant معناه
+
+243
+00:16:06,630 --> 00:16:10,830
+lysine deamination positive اللون الـ purple في الـ butt
+
+244
+00:16:10,830 --> 00:16:13,610
+معناه lysine decarboxylation positive وإحنا قلنا
+
+245
+00:16:13,610 --> 00:16:16,510
+ممنوع البكتيريا تعمل الأثنين مع بعض يبقى اللون الـ
+
+246
+00:16:16,510 --> 00:16:19,170
+red في الـ slant و الـ purple بالـ butt ممنوع أني أنا
+
+247
+00:16:19,170 --> 00:16:27,380
+أشوفه هذه تفسير النتائج هنا نلاحظ أن اللون purple
+
+248
+00:16:27,380 --> 00:16:31,880
+في الإسلاند و purple في الـ butt يبقى K على K طبعا
+
+249
+00:16:31,880 --> 00:16:35,200
+تفسير النتائج زي ما قلنا بما أن purple و purple
+
+250
+00:16:35,200 --> 00:16:39,720
+purple في الإسلاند يبقى Gelatin purple في الإسلاند
+
+251
+00:16:39,720 --> 00:16:45,900
+يبقى Gelatin deamination purple في الـ butt
+
+252
+00:16:45,900 --> 00:16:51,590
+يبقى Lysine decarboxylation هنا K على A نلاحظ أنه
+
+253
+00:16:51,590 --> 00:16:54,790
+هنا بما أنه في الـ Butt A يبقى Glucose fermentation
+
+254
+00:16:54,790 --> 00:17:01,830
+في الـ slant هنا K يبقى هي في الـ slant تتحول للون
+
+255
+00:17:01,830 --> 00:17:04,950
+للون Violet يبقى هي Gelatin Deamination يبقى هنا
+
+256
+00:17:04,950 --> 00:17:07,190
+البكتيريا ماعندها القدرة أن تتعمل للـ ليسين ولا
+
+257
+00:17:07,190 --> 00:17:11,950
+Deamination ولا Decarboxylation هنا الرمز في عندي
+
+258
+00:17:11,950 --> 00:17:14,610
+Yellow في الـ Butt يبقى Glucose fermentation
+
+259
+00:17:14,610 --> 00:17:17,410
+positive ليسين Decarboxylation negative في عندي
+
+260
+00:17:17,410 --> 00:17:23,280
+تشققات ممكن نضيف Gas في عندي هان لون Red في الـ ..
+
+261
+00:17:23,280 --> 00:17:25,900
+اللي هو في الـ Slant يبقى عاملة الـ Bacteria
+
+262
+00:17:25,900 --> 00:17:33,360
+Lysine Deamination هان في عندي H2S اللي هو H2S
+
+263
+00:17:33,360 --> 00:17:37,680
+لأنه اللي اتكون اللون الأسود الـ Plus هاي معناها
+
+264
+00:17:37,680 --> 00:17:41,200
+أنه في عندي تشققات نسينا نقول في الـ Triple Sugar
+
+265
+00:17:41,200 --> 00:17:44,700
+Iron Agar ممكن أضيف رمز الجاز و ممكن أضيف رمز الـ
+
+266
+00:17:44,700 --> 00:17:47,880
+Plus معناه أنه والله في عندي اتكون غاز يبقى رمز
+
+267
+00:17:47,880 --> 00:17:51,950
+الـ Plus معناه في عندي اللي هو اتكون نتيجة
+
+268
+00:17:51,950 --> 00:17:55,950
+Fermentation acid with gas يبقى الـ plus ممكن أرمز
+
+269
+00:17:55,950 --> 00:18:00,390
+الـ plus ممكن أرمز اللي هو الـ gas طيب فوق في الـ
+
+270
+00:18:00,390 --> 00:18:04,530
+slant اتكون اللون اللي هو الـ red يبقى صار في عندي
+
+271
+00:18:04,530 --> 00:18:10,370
+اللي هو Lysine deamination طيب ليش أنا كتبت A؟
+
+272
+00:18:10,370 --> 00:18:13,930
+ليش أنا كتبت A؟ إحنا قلنا في هذه الحالة بما أنه
+
+273
+00:18:13,930 --> 00:18:20,320
+اتكون H2S gas ما أقدر أكتب A ولا K هان بقول لي في
+
+274
+00:18:20,320 --> 00:18:24,320
+هذه الحالة بما أن البكتيريا عملت ليسين D-amination
+
+275
+00:18:24,320 --> 00:18:27,500
+كانت أعرفه أكيد مش هتكون عاملة ليسين D-
+
+276
+00:18:27,500 --> 00:18:31,540
+carboxylation فبالتالي برمز للرمز A يبقى في هذه
+
+277
+00:18:31,540 --> 00:18:34,780
+الحالة إحنا قلنا الـ H2S بما أنه اتكون في الـ
+
+278
+00:18:34,780 --> 00:18:39,280
+Triple Sugar Iron Agar يبقى ما بقدر أحدد A
+
+279
+00:18:39,280 --> 00:18:43,640
+أو K لأن الـ H2S production بيصير في ظروف acidic
+
+280
+00:18:43,640 --> 00:18:46,620
+condition الـ A فعلا صار في Glucose fermentation
+
+281
+00:18:47,230 --> 00:18:49,710
+بالنسبة للـ K في الأصل هي عملت Glucose fermentation
+
+282
+00:18:49,710 --> 00:18:53,550
+لما بعدين عملت Lysine decarboxylation لكن هنا أنا
+
+283
+00:18:53,550 --> 00:18:58,990
+كتبت A لأن الرمز الـ R اللي هو الـ R مع أنها صار
+
+284
+00:18:58,990 --> 00:19:01,650
+عندي Lysine deamination لما أنها عملت Lysine
+
+285
+00:19:01,650 --> 00:19:04,670
+deamination يبقى مع عملت Lysine decarboxylation
+
+286
+00:19:04,670 --> 00:19:07,970
+يبقى فقط بيكون عندي اللي تحت اللي هو الـ Glucose
+
+287
+00:19:07,970 --> 00:19:11,270
+fermentation هاي برضه منضيف اللي هو الـ plus R
+
+288
+00:19:11,270 --> 00:19:16,200
+الغاز عليها لأنه في عندي تشققات نخلصنا هيك
+
+289
+00:19:16,200 --> 00:19:20,040
+ميديات اللي هي Lysine Iron Agar  نيجي للميديا
+
+290
+00:19:20,040 --> 00:19:24,280
+التانية اللي هي الـ Motility Indole Ornithine MIO
+
+291
+00:19:24,280 --> 00:19:29,340
+بما أن في عندي Motility يبقى هتكون Stab  هذه
+
+292
+00:19:29,340 --> 00:19:33,300
+الميديا مش هكشف على الـ H2S Gas هتوفر لي Aerobic
+
+293
+00:19:33,300 --> 00:19:36,860
+Condition فمن خلالها هكشف على الـ Indole وعلى الـ
+
+294
+00:19:36,860 --> 00:19:39,660
+Ornithine Deamination أي الـ Deamination بيصير في
+
+295
+00:19:39,660 --> 00:19:42,830
+ظروف Aerobic Condition بالنسبة للـ Anaerobic
+
+296
+00:19:42,830 --> 00:19:45,030
+Condition قلنا الـ Glucose fermentation والـ
+
+297
+00:19:45,030 --> 00:19:48,170
+decarboxylation بيصير في الـ Anaerobic Condition
+
+298
+00:19:48,170 --> 00:19:52,510
+الـ pH Indicator نفس ميديا ليسين ايرون اجار
+
+299
+00:19:52,510 --> 00:19:58,490
+اللي هي البروموكريزول بيربل بتكون لون Yellow في الـ
+
+300
+00:19:58,490 --> 00:20:01,430
+acid condition في الـ basic condition بتكون لون
+
+301
+00:20:01,430 --> 00:20:05,610
+purple في الـ neutral بيكون لون أخضر طيب إحنا قلنا
+
+302
+00:20:05,610 --> 00:20:10,370
+في تحت لو أنا شفت لون أصفر معناه أنه البكتيريا
+
+303
+00:20:10,370 --> 00:20:14,250
+عملت Glucose fermentation اتكون الـ acid حول لون
+
+304
+00:20:14,250 --> 00:20:19,010
+الـ pH indicator للون الـ yellow طب لو أنا شفت
+
+305
+00:20:19,010 --> 00:20:22,030
+تحت لون purple معناه أنه البكتيريا عملت
+
+306
+00:20:22,030 --> 00:20:26,250
+decarboxylation للأورنثين اتكون alkaline product و
+
+307
+00:20:26,250 --> 00:20:30,650
+حولت الـ media للون purple طب على السطح لو أنا
+
+308
+00:20:30,650 --> 00:20:34,420
+ضفت الـ Kovac's reagent يبقى البكتيريا عندها إنزيم
+
+309
+00:20:34,420 --> 00:20:38,000
+لتربتوفاناز هيدروجيناز لتربتوفان اتكون اندول ارتبط مع
+
+310
+00:20:38,000 --> 00:20:42,500
+الـ Kovac's reagent تتكون Red ring طب لو كان على
+
+311
+00:20:42,500 --> 00:20:45,120
+سطح لون فيه purple معناه أن البكتيريا عملت
+
+312
+00:20:45,120 --> 00:20:48,700
+deamination للأورنثين اتكون اندول alkaline product
+
+313
+00:20:48,700 --> 00:20:52,840
+حول الـ pH indicator للون الـ purple هذا بالنسبة
+
+314
+00:20:52,840 --> 00:20:57,870
+لهذه الـ media نشوف مكونات الـ media أكيد yeast extract
+
+315
+00:20:57,870 --> 00:21:04,090
+كمصدر للتغذية peptone تمام لأن البكتيريا هتعمل الـ
+
+316
+00:21:04,090 --> 00:21:11,110
+الـ deamination للـ peptone  sorry في هذه الفقرة نمسح
+
+317
+00:21:11,110 --> 00:21:16,270
+الـ ornithine deamination و نكتب peptone deamination
+
+318
+00:21:16,270 --> 00:21:20,830
+هان
+
+319
+00:21:20,830 --> 00:21:23,710
+Peptone deamination مش decarboxylation
+
+320
+00:21:26,080 --> 00:21:29,500
+مش ornithine هان اللي هي الـ peptone deamination و
+
+321
+00:21:29,500 --> 00:21:33,080
+هان اللي هي برضه الـ peptone deamination تمام؟ بدي
+
+322
+00:21:33,080 --> 00:21:36,100
+أكشف في هذه الـ media عن قدرة البكتيريا أنها تعمل
+
+323
+00:21:36,100 --> 00:21:38,680
+decarboxylation للـ ornithine لو ماعملت
+
+324
+00:21:38,680 --> 00:21:42,120
+decarboxylation هتعمل deamination للـ peptone في الـ
+
+325
+00:21:42,120 --> 00:21:42,960
+Anaerobic condition
+
+326
+00:21:46,330 --> 00:21:48,710
+بالنسبة لأ ornithine موجود أكيد في الـ media
+
+327
+00:21:48,710 --> 00:21:51,430
+لأنها أكشف عن قدرة البكتيريا أن تعمل دي
+
+328
+00:21:51,430 --> 00:21:54,650
+carboxylation الـ Dextrose موجود بكمية قليلة عشان
+
+329
+00:21:54,650 --> 00:21:58,410
+أكشف على الـ fermentation الأجار بما أن فحص
+
+330
+00:21:58,410 --> 00:22:02,350
+Motility يبقى هي semi-solid media نسبة الأجار 4 من
+
+331
+00:22:02,350 --> 00:22:08,230
+10% الـ pH indicator الـ بروموكريزول بيربل قلنا
+
+332
+00:22:08,230 --> 00:22:13,020
+حدوده شو بتكون في الـ acidic و الـ basic الـ MIO
+
+333
+00:22:13,020 --> 00:22:18,880
+media تستخدم عشان إني أنا أفرق اللي هو الـ أو أكشف
+
+334
+00:22:18,880 --> 00:22:22,740
+على تلت فحوصات اللي هي في خلال one culture two
+
+335
+00:22:22,740 --> 00:22:25,920
+motility Indole production and ornithine
+
+336
+00:22:25,920 --> 00:22:29,250
+decarboxylation بالنسبة لـ Motility قلنا بنعمل
+
+337
+00:22:29,250 --> 00:22:33,390
+Stabbing في الـ Semi Solid Media باستخدام الـ
+
+338
+00:22:33,390 --> 00:22:36,750
+Straight Wire من خلال اللي هو الـ Inoculating
+
+339
+00:22:36,750 --> 00:22:42,010
+Needle لو البكتيريا Motile بقول لي أكيد هـ will
+
+340
+00:22:42,010 --> 00:22:45,430
+migrate from the stab line by means of their
+
+341
+00:22:45,430 --> 00:22:49,610
+flagella البكتيريا هتتحرك بعيدا عن خط الزراعة
+
+342
+00:22:49,610 --> 00:22:52,890
+باستخدام الفلاجلة producing turbidity or
+
+343
+00:22:52,890 --> 00:22:54,950
+cloudiness through other media هيتكون عندك
+
+344
+00:22:54,950 --> 00:22:58,320
+turbidity و cloudiness في الميديا الـ Non-motile
+
+345
+00:22:58,320 --> 00:23:03,180
+organism هتنمو فقط على خط الزراعة فبالتالي باقي الـ
+
+346
+00:23:03,180 --> 00:23:07,940
+media هتكون clear بالنسبة للفحص الثاني اللي هو الـ
+
+347
+00:23:07,940 --> 00:23:12,460
+Ornithine بقول لي هذه الـ media بدي أكشف على وجود
+
+348
+00:23:12,460 --> 00:23:15,800
+إنزيم الـ Ornithine decarboxylase هل البكتيريا عندها
+
+349
+00:23:15,800 --> 00:23:20,280
+القدرة أنها تعمل decarboxylation للـ Ornithine ولا لأ
+
+350
+00:23:20,280 --> 00:23:24,730
+باستخدام اللي هو الـ Ornithine الموجود في الـ media لو
+
+351
+00:23:24,730 --> 00:23:27,690
+البكتيريا عندها هذا الإنزيم بقول لي هذا الإنزيم
+
+352
+00:23:27,690 --> 00:23:31,450
+الـ Ornithine decarboxylase نفس الـ Lysine decarboxylase
+
+353
+00:23:31,450 --> 00:23:35,830
+بتنشط في الـ acidic environment زي ما قلنا created
+
+354
+00:23:35,830 --> 00:23:39,570
+by the initial fermentation of the Glucose من وين
+
+355
+00:23:39,570 --> 00:23:43,310
+بيجي الـ acidic environment اللي هو الـ
+
+356
+00:23:43,310 --> 00:23:46,490
+acidic environment acidic information أو الـ acidic
+
+357
+00:23:46,490 --> 00:23:49,710
+اللي هو الـ acidic condition أو الـ acidic
+
+358
+00:23:49,710 --> 00:23:54,030
+environment بقول لي الـ acidic environment بيكون يصير
+
+359
+00:23:54,030 --> 00:23:56,870
+في الأصل من خلال أن البكتيريا بالأول بتعمل
+
+360
+00:23:56,870 --> 00:24:01,050
+fermentation للجلوكوز تكون عندي acidic condition
+
+361
+00:24:01,050 --> 00:24:06,110
+فبالتالي هيتحفظ لإنتاج اللي هو أو لعمل الـ
+
+362
+00:24:06,110 --> 00:24:09,510
+decarboxylation للأورنثين نفس فكرة الـ Lysine
+
+363
+00:24:09,510 --> 00:24:13,650
+decarboxylase قلنا بيصير في acidic condition عشان
+
+364
+00:24:13,650 --> 00:24:16,830
+هيك ممكن إني أنا أشوف الـ H2S production جنبه
+
+365
+00:24:16,830 --> 00:24:21,390
+alkaline أو acidic بقول لي الـ amino acid لو صاروا
+
+366
+00:24:21,390 --> 00:24:22,510
+decarboxylated
+
+367
+00:24:26,500 --> 00:24:30,020
+المنتج الناتج اللي هو المنتج الناتج اللي هو
+
+368
+00:24:30,020 --> 00:24:32,520
+المنتج الناتج اللي هو المنتج الناتج اللي هو المنتج
+
+369
+00:24:32,520 --> 00:24:33,020
+الناتج اللي هو المنتج الناتج اللي هو المنتج الناتج
+
+370
+00:24:33,020 --> 00:24:35,520
+اللي هو المنتج الناتج اللي هو المنتج الناتج اللي هو
+
+371
+00:24:35,520 --> 00:24:39,060
+المنتج الناتج اللي هو المنتج الناتج اللي هو
+
+372
+00:24:39,060 --> 00:24:42,680
+المنتج
+
+373
+00:24:42,680 --> 00:24:43,500
+الناتج اللي هو المنتج الناتج اللي هو المنتج الناتج
+
+374
+00:24:43,500 --> 00:24:43,660
+المنتج الناتج اللي هو المنتج الناتج اللي هو المنتج
+
+375
+00:24:43,660 --> 00:24:48,880
+النتائج Lysine Decarboxylase في ميديا Lysine Iron
+
+376
+00:24:48,880 --> 00:24:53,580
+Agar كان المركب الناتج من الـ Lysine Decarboxylase
+
+377
+00:24:53,580 --> 00:24:57,620
+الكادافيرين تمام لكن هان هذا هو المركب الناتج من
+
+378
+00:24:57,620 --> 00:25:02,670
+عملية الـ Ornithine Decarboxylation بقول لي هيرفع
+
+379
+00:25:02,670 --> 00:25:05,650
+الـ pH هيتكون عندي alkaline product فبالتالي
+
+380
+00:25:05,650 --> 00:25:09,690
+هيتحول لون الـ media للون dark purple لو البكتيريا
+
+381
+00:25:09,690 --> 00:25:11,930
+ما عندها القدرة أو ما عندها الإنزيم في الأصل
+
+382
+00:25:11,930 --> 00:25:16,710
+فبالتالي هيظل أسدك الناتج من عملية الـ fermentation
+
+383
+00:25:16,710 --> 00:25:20,970
+لجلوكوز فهيتحول لون الـ media في اللي هو الـ butt في
+
+384
+00:25:20,970 --> 00:25:22,750
+تحت هيتحول للون الأصفر
+
+385
+00:25:27,250 --> 00:25:30,130
+أخر شيء فحص الاندول باكشف عن وجود اللي هو الـ
+
+386
+00:25:30,130 --> 00:25:33,670
+tryptophan الـ tryptophan موجود في الميديا في اللي
+
+387
+00:25:33,670 --> 00:25:38,510
+هو الـ peptone تمام بالنسبة للـ positive result
+
+388
+00:25:38,510 --> 00:25:43,630
+هيتحول لون اللي هو في الـ على السطح Red color اللي
+
+389
+00:25:43,630 --> 00:25:46,910
+هو الـ cherry red color هذا معناه أن البكتيريا
+
+390
+00:25:46,910 --> 00:25:49,530
+عندها إنزيم لـ tryptophan بعد إضافة اللي هو
+
+391
+00:25:49,530 --> 00:25:53,130
+الـ Kovac's reagent  لكن الـ negative result مش
+
+392
+00:25:53,130 --> 00:26:00,430
+هيتغير أي لون طب نيجي للنتائج بس
+
+393
+00:26:00,430 --> 00:26:07,190
+بالنسبة للنتائج ها يندي تلت نتائج لكن طبعا هن واحد
+
+394
+00:26:07,190 --> 00:26:10,230
+و واحد هي نفس الشيء لكن هن بعد إضافة الـ Kovac's
+
+395
+00:26:10,230 --> 00:26:16,370
+reagent هن اثنين و هن ثلاثة نيجي بدنا أول شيء اللي هو
+
+396
+00:26:16,370 --> 00:26:21,870
+نكشف أو نقرأ نتيجة فحص الـ motility تمام بعدين هقرأ
+
+397
+00:26:21,870 --> 00:26:27,490
+نتيجة الـ Indole أو الـ Decarboxylase اللي هو الـ
+
+398
+00:26:27,490 --> 00:26:30,810
+Ornithine Decarboxylase بعدين هقرأ اللي هو هضيف الـ
+
+399
+00:26:30,810 --> 00:26:35,410
+Kovac's reagent و هقرأ نتيجة اللي هو الـ Indole test
+
+400
+00:26:35,410 --> 00:26:43,190
+بالنسبة لأول اللي هو الـ tube أول
+
+445
+00:29:59,900 --> 00:30:01,920
+عملت هاك يبقى هي في الأصل جليكوز fermentation
+
+446
+00:30:01,920 --> 00:30:05,100
+positive لأنه احنا قلنا الـ decarboxylation بيصير
+
+447
+00:30:05,100 --> 00:30:08,770
+في acidic environment. الـ Indol بما أنه اتحول أو
+
+448
+00:30:08,770 --> 00:30:12,170
+اتكون حلقة حمراء يبقى هي positive. ليش تغير لونها
+
+449
+00:30:12,170 --> 00:30:15,310
+للون أصفر. نفس الفكرة، الـ Kovac's reaction بتكون من
+
+450
+00:30:15,310 --> 00:30:20,510
+HCl، هيتحول الوسط لوسط حمضي، وهيحول لون الـ pH
+
+451
+00:30:20,510 --> 00:30:25,470
+indicator للون Yellow. هيك بنكون خلصنا اللي هو
+
+452
+00:30:25,470 --> 00:30:29,730
+Chapter Single Media Multiple Test. يعطيكم العافية
+
+453
+00:30:29,730 --> 00:30:31,990
+والسلام عليكم ورحمة الله وبركاته
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/_HOd9t_eBH4_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/_HOd9t_eBH4_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..f084ae84d4b76da13d0ab34d815dcd0494852387
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/_HOd9t_eBH4_postprocess.srt
@@ -0,0 +1,1812 @@
+1
+00:00:01,890 --> 00:00:04,990
+من الأمثلة الأخرى على single media multiple test
+
+2
+00:00:04,990 --> 00:00:10,690
+ليسين آيرون أجار ليسين آيرون أجار من اسمها أكيد في
+
+3
+00:00:10,690 --> 00:00:14,690
+الميديا في عندي ليسين الليسين يعتبر amino acid في
+
+4
+00:00:14,690 --> 00:00:18,290
+الميديا هى بدي أكشف هل البكتيريا بتعمل deamination
+
+5
+00:00:18,290 --> 00:00:23,190
+للليسين او decarboxylation للليسين آيرون بما أنه
+
+6
+00:00:23,190 --> 00:00:26,830
+في آيرون في اسم الميديا يبقى في عندي H2S
+
+7
+00:00:26,830 --> 00:00:31,720
+production بدي أكشف على الـH2S أجار يبقىالـ
+
+8
+00:00:31,720 --> 00:00:35,440
+Solidifying agent الموجود في الميديا هو الأجار
+
+9
+00:00:35,440 --> 00:00:42,120
+بنسبة 1.5% فبالتالي هصب الميديا في Slant YouTube
+
+10
+00:00:42,120 --> 00:00:46,560
+عشان أوفر European condition من خلال Slant لأنه
+
+11
+00:00:46,560 --> 00:00:49,120
+بدي أكشف في هذه الميديا على الـ deamination
+
+12
+00:00:49,120 --> 00:00:53,800
+للليسين والـ deamination للجيلاتين وهدول بيسيروا
+
+13
+00:00:53,800 --> 00:00:59,090
+في الـ European condition كمان بدي أنا أكشف علىالـ
+
+14
+00:00:59,090 --> 00:01:02,130
+Decarboxylation للليسين والـ Fermentation للجليكوز
+
+15
+00:01:02,130 --> 00:01:05,350
+وهدول بيصيروا في الآن Europe Condition اللي من
+
+16
+00:01:05,350 --> 00:01:10,030
+خلال البط بيوفر هاي الظروف في هاي الميديا في
+
+17
+00:01:10,030 --> 00:01:12,830
+Indicator بما أنه بكشف على ال fermentation
+
+18
+00:01:12,830 --> 00:01:17,730
+للجليكوز فأكيد لازم وكمان هكشف على ال Deamination
+
+19
+00:01:17,730 --> 00:01:21,310
+للليسين أو الجيلاتين فبالتالي هيتكون acidic or
+
+20
+00:01:21,310 --> 00:01:25,920
+alkaline product فلازم يكون في عندي BH Indicatorال
+
+21
+00:01:25,920 --> 00:01:29,500
+بي اتش انديكاتور الموجود في هاي الميديا اول مرة
+
+22
+00:01:29,500 --> 00:01:34,320
+يمر معنا برامو سارزال بيربل هذا ال بي اتش
+
+23
+00:01:34,320 --> 00:01:38,260
+انديكاتور في ال acidic condition بيكون لونه يلو في
+
+24
+00:01:38,260 --> 00:01:41,320
+ال basic condition بيكون لونه بيربل في ال neutral
+
+25
+00:01:41,320 --> 00:01:46,670
+بيكون لونه اجري او بيكون لونه فيولت فاتحقلنا في
+
+26
+00:01:46,670 --> 00:01:50,750
+عندي file media هكشف على ال H2S production فأكيد
+
+27
+00:01:50,750 --> 00:01:55,390
+لازم يكون H2S indicator اللي هو الferric ammonium
+
+28
+00:01:55,390 --> 00:02:00,710
+citrate تمام هذا بالنسبة لمكونات ال media وعن شوه
+
+29
+00:02:00,710 --> 00:02:05,310
+بتكشف ال media أول أشياء البكتيريا هتلجأ لإله
+
+30
+00:02:05,310 --> 00:02:10,990
+كمصدر للطاقة و للغضاء اللي هو الجليكوز فلو
+
+31
+00:02:10,990 --> 00:02:13,370
+البكتيريا عندها القدرة انها تعمل fermentation
+
+32
+00:02:13,370 --> 00:02:19,170
+للجليكوزهيتكون عندى product acidic هيتحول لون ال
+
+33
+00:02:19,170 --> 00:02:23,770
+media للون yellow يبقى في حال كان في عندي جليكوز
+
+34
+00:02:23,770 --> 00:02:28,770
+fermentation positive يبقى هيتحول لون البط بما انه
+
+35
+00:02:28,770 --> 00:02:33,270
+صار في ظروف لاهوائية هيتحول لون البط للون yellow
+
+36
+00:02:33,270 --> 00:02:37,290
+تانى إشي خلص الجليكوز الجليكوز موجود في ال media
+
+37
+00:02:37,290 --> 00:02:41,110
+بكمية قليلة نفس ل triple sugar iron agar موجود
+
+38
+00:02:41,110 --> 00:02:46,770
+بالنسبة 1 من 10% فالبكتيريافي حال انه خلص
+
+39
+00:02:46,770 --> 00:02:51,030
+الجليكواز اكيد هتلجأ لمصادر اخرى للحصول على الطاقة
+
+40
+00:02:51,030 --> 00:02:55,970
+و للتغذية فبالتالي هتروح للايسين اللايسين موجود في
+
+41
+00:02:55,970 --> 00:02:58,610
+ال media وهي ال media انا بدي اكشف عن قدرة
+
+42
+00:02:58,610 --> 00:03:01,610
+البكتيريا انها تعمل deamination للايسين او
+
+43
+00:03:01,610 --> 00:03:06,370
+decarboxylation او اذا لاحظين انه مكتوب او ممنوع
+
+44
+00:03:06,370 --> 00:03:10,710
+ان البكتيريا يكون عندها القدرة انها تعمل للايسين
+
+45
+00:03:10,710 --> 00:03:14,400
+deamination و decarboxylation مع بعضإما هتعمل de
+
+46
+00:03:14,400 --> 00:03:17,900
+-amination أو de-carboxylation أو ممكن ماتعمل أي
+
+47
+00:03:17,900 --> 00:03:20,840
+إشي لأ اللايسين لكن الأثنين مع بعض ممنوع أني أنا
+
+48
+00:03:20,840 --> 00:03:25,360
+أشوفهم هلأ ال de-amination لالليسين هيتكون عندي
+
+49
+00:03:25,360 --> 00:03:30,340
+alkaline product تمام المفترض بما أنه اتكون عندي
+
+50
+00:03:30,340 --> 00:03:34,540
+alkaline product هيرفع ال pH هيتحول لون ال media
+
+51
+00:03:34,540 --> 00:03:38,020
+للون purple لكن إذا من اللحظة إذا بتلاحظوا مكتوب
+
+52
+00:03:38,020 --> 00:03:42,480
+هنا بتحول لون ال media للون redطيب ليش اتحول لون
+
+53
+00:03:42,480 --> 00:03:45,460
+ال media للون Red بالرغم انه اتكون عندى alkaline
+
+54
+00:03:45,460 --> 00:03:50,560
+product وفيه عندى BH Indicator موجود في ال acid في
+
+55
+00:03:50,560 --> 00:03:53,040
+sorry في ال basic condition بيكون لونه purple
+
+56
+00:03:53,040 --> 00:03:57,140
+بقوللي ال alkaline product الناتج من عملية ال
+
+57
+00:03:57,140 --> 00:04:02,560
+lysine deamination قوة ارتباطه بال H2S Indicator
+
+58
+00:04:02,560 --> 00:04:06,140
+أعلى من قوة ارتباطه بال BH Indicator فبالتالي
+
+59
+00:04:06,140 --> 00:04:10,130
+هيتكون اللون ال Redنتيجة أن الـAlkaline Product
+
+60
+00:04:10,130 --> 00:04:16,530
+لـLysine Deamination قوة ارتباطه بالـH2S Indicator
+
+61
+00:04:16,530 --> 00:04:21,690
+أعلى من الـBH Indicator فهيتحول اللون للون Red طب
+
+62
+00:04:21,690 --> 00:04:23,830
+لو البكتيريا عندها القدرة انها تعمل
+
+63
+00:04:23,830 --> 00:04:26,610
+Decarboxylation للليسين وما عندها القدرة انها تعمل
+
+64
+00:04:26,610 --> 00:04:31,130
+Deamination للليسين نتيجة عملية الـDecarboxylation
+
+65
+00:04:31,130 --> 00:04:35,210
+برضه هيتثور ان عندي Alkaline Productموجود في عندي
+
+66
+00:04:35,210 --> 00:04:38,150
+pH Indicator في ال basic condition بيكون لونه
+
+67
+00:04:38,150 --> 00:04:42,950
+purple فهي تحول لون ال media للون purple طيب لو
+
+68
+00:04:42,950 --> 00:04:46,110
+البكتيريا ماعندها القدرة انها تعمل لليسين اي اشي
+
+69
+00:04:46,110 --> 00:04:49,370
+سواء deamination او decarboxylation بقوللي هيروح
+
+70
+00:04:49,370 --> 00:04:52,810
+هتروح البكتيريا لأ الجيلاتين الموجود في ال media
+
+71
+00:04:52,810 --> 00:04:56,430
+يعتبر protein هتعمله deamination هيتكون عندي
+
+72
+00:04:56,430 --> 00:05:00,780
+alkaline productموجود عندي pH Indicator في ال
+
+73
+00:05:00,780 --> 00:05:06,140
+basic condition اللي هو لونه purple فبالتالي هيرفع
+
+74
+00:05:06,140 --> 00:05:09,360
+ال pH هذا ال alkaline product هيتحول لون ال media
+
+75
+00:05:09,360 --> 00:05:15,500
+للون purple طيب نيجي للنتائج هلأ ال media فيه عندي
+
+76
+00:05:15,500 --> 00:05:18,980
+slant وbutt قولنا ال slant بتوفر ظروف aerobic
+
+77
+00:05:18,980 --> 00:05:22,080
+condition فالbutt بتوفر ظروف uneurobic condition
+
+78
+00:05:22,610 --> 00:05:26,730
+هلأ بمسك اليوتيوب بتطلع على ال slant لو اتكوّن لون
+
+79
+00:05:26,730 --> 00:05:31,070
+red يبقى بحكي انه صار في عندي Lycine deamination
+
+80
+00:05:31,070 --> 00:05:35,550
+برمز إيه بالرمز A لو اتكوّن لون purple بحكي صار في
+
+81
+00:05:35,550 --> 00:05:40,690
+عندي جيلاتين deamination برمز لإيه بالرمز K لأنه
+
+82
+00:05:40,690 --> 00:05:43,370
+إحنا قلنا اللي Lycine deamination والجيلاتين
+
+83
+00:05:43,370 --> 00:05:48,140
+deamination بيصيروا في ظروف aerobic conditionوقلنا
+
+84
+00:05:48,140 --> 00:05:51,760
+ناتج عملية الـ glycine deamination اللي هو بـ
+
+85
+00:05:51,760 --> 00:05:55,660
+alkaline ال product قوة ارتباطه بالـ H2S Indicator
+
+86
+00:05:55,660 --> 00:05:58,340
+أعلى من الـ BH Indicator فبالتالي هيتحول اللون
+
+87
+00:05:58,340 --> 00:06:01,320
+للون Red الجيلاتين دي amination قولنا بتكون
+
+88
+00:06:01,320 --> 00:06:04,520
+alkaline ال product بيرفع ال BH فبتتحول لون ال
+
+89
+00:06:04,520 --> 00:06:08,520
+media للون بارد طيب بالنسبة للبط قولنا بتوفر Un
+
+90
+00:06:08,520 --> 00:06:10,580
+-Irritable Condition بتكشف على الجليكوز
+
+91
+00:06:10,580 --> 00:06:13,200
+fermentation والـ glycine decarboxylation إذا
+
+92
+00:06:13,200 --> 00:06:17,090
+اتحول لون البط للون yellow يبقى back topبفهم إنه
+
+93
+00:06:17,090 --> 00:06:20,270
+صار في عندي جليكوز fermentation و برمز لإله بالرمز
+
+94
+00:06:20,270 --> 00:06:24,570
+A لو اتحول لون البطل لون purple يبقى بعرف إنه صار
+
+95
+00:06:24,570 --> 00:06:28,150
+في عندي ليسين D-carboxylation و برمز لإله بالرمز K
+
+96
+00:06:28,150 --> 00:06:33,230
+طيب بالنسبة ل ال H2O acid production قولنا ال H2O
+
+97
+00:06:33,230 --> 00:06:35,670
+acid production بيصير في ظروف un-aerobic condition
+
+98
+00:06:35,670 --> 00:06:39,660
+و بيصير في ظروف acidic conditionلو اتكون عندى الـ
+
+99
+00:06:39,660 --> 00:06:43,740
+H2S production مش هعرف هل البكتيريا عملت Gelicose
+
+100
+00:06:43,740 --> 00:06:47,560
+fermentation او Lysine decarboxylation ممكن تكون
+
+101
+00:06:47,560 --> 00:06:50,800
+عملت Gelicose fermentation وممكن تكون عملت Lysine
+
+102
+00:06:50,800 --> 00:06:54,100
+decarboxylation طيب ليش ماقولنا زى ميديا تلثر
+
+103
+00:06:54,100 --> 00:06:57,840
+بالشوبر ايرون اجار من حد ما اشوف الـH2S production
+
+104
+00:06:57,840 --> 00:07:01,340
+بكتب جنبها رمز الـA ممنوع اني انا اشوف رمز الـK
+
+105
+00:07:01,340 --> 00:07:04,880
+بقوللي لأ في هى ال media ممكن يكون A وممكن يكون K
+
+106
+00:07:04,880 --> 00:07:09,820
+ممكن يكون A وممكن يكون Kيبقى الـ H2O production
+
+107
+00:07:09,820 --> 00:07:13,700
+ممكن لأنها تكون عملت Gelicose fermentation رمزها
+
+108
+00:07:13,700 --> 00:07:18,020
+اللى اللى هو البط ممكن يكون ال ICD-carboxylation
+
+109
+00:07:18,020 --> 00:07:23,700
+كام لش هلأ ال ال ال بكتيريا اللى عندها القدرة لو
+
+110
+00:07:23,700 --> 00:07:27,780
+كان الرمز هلأ اه ومعها H2O production معناه ان
+
+111
+00:07:27,780 --> 00:07:31,180
+البكتيريا عملت Gelicose fermentation اتكون عندى
+
+112
+00:07:31,180 --> 00:07:35,080
+acidic condition فبالتالي صار في عندى H2O
+
+113
+00:07:35,080 --> 00:07:38,800
+productionبالنسبة للـ Lysin-D Carboxylation هذا
+
+114
+00:07:38,800 --> 00:07:41,940
+بتكون alkaline product واحنا قلنا الـ H2S
+
+115
+00:07:41,940 --> 00:07:46,400
+production بيحصل في ظروف acidic condition كيف
+
+116
+00:07:46,400 --> 00:07:49,640
+بنحكي أنه عادي ممكن أني أنا أشوف الـ H2S
+
+117
+00:07:49,640 --> 00:07:52,200
+production مع الكائنات، مع الـ Lysin-D
+
+118
+00:07:52,200 --> 00:07:56,320
+Carboxylation؟ بقولي قبل ما البكتيريا تعمل Lysin-D
+
+119
+00:07:56,320 --> 00:07:59,640
+Carboxylation بتكون في الأصل عملت Gelicose
+
+120
+00:07:59,640 --> 00:08:04,800
+fermentationعملت جليكوس fermentation تمام اتكون
+
+121
+00:08:04,800 --> 00:08:07,800
+عندي acidic condition هذا ال acidic condition
+
+122
+00:08:07,800 --> 00:08:12,040
+اتوفر فبالتالي البكتيريا عملت H2O production وعملت
+
+123
+00:08:12,040 --> 00:08:14,660
+lysine decarboxylation لأن ال lysine
+
+124
+00:08:14,660 --> 00:08:18,180
+decarboxylation بيصير برضه نفس ال H2O production
+
+125
+00:08:18,180 --> 00:08:22,920
+في ظروف acidic condition ال lysine decarboxylation
+
+126
+00:08:22,920 --> 00:08:27,880
+نفس ال H2O production بيحفظ في ظروف acidic
+
+127
+00:08:27,880 --> 00:08:32,000
+conditionفبالتالي من حد ما اشوف ان والله البكتيريا
+
+128
+00:08:32,000 --> 00:08:35,100
+صار في عندها او عملة lysin-d-carboxylation ده وهي
+
+129
+00:08:35,100 --> 00:08:37,540
+بالاصل بتكون قبلها عاملة gelicose fermentation
+
+130
+00:08:37,540 --> 00:08:41,900
+اتكون عندى acidic condition اتحفظ ال lysin-d
+
+131
+00:08:41,900 --> 00:08:45,660
+-carboxylation واتحفظ اللى هو ال H2S production
+
+132
+00:08:45,660 --> 00:08:48,340
+اللى لازم يلاقيلهم اتنين acidic condition اللى
+
+133
+00:08:48,340 --> 00:08:51,180
+ناتج من عملية ال gelicose fermentation يبقى في هاي
+
+134
+00:08:51,180 --> 00:08:55,520
+الميديا لو عندى اتكون ال H2Sممكن تكون في الأصل هي
+
+135
+00:08:55,520 --> 00:08:58,680
+Gelicose Fermentation A وممكن تكون رمزها K اللي هي
+
+136
+00:08:58,680 --> 00:09:01,560
+عملت Lycine Decarboxylation وقبل عملت Gelicose
+
+137
+00:09:01,560 --> 00:09:07,100
+Fermentation طيب مكونات ال media yeast extract
+
+138
+00:09:07,100 --> 00:09:10,740
+لازم لنمو البكتيريا Dextrose اللي هو موجود بكمية
+
+139
+00:09:10,740 --> 00:09:15,100
+قليلة 1 من 10% عشان البكتيريا تعمل fermentation ال
+
+140
+00:09:15,100 --> 00:09:17,560
+Lycine اللي هو البكتيريا اللي هيكشف هل هتعمله
+
+141
+00:09:17,560 --> 00:09:21,160
+decarboxylation أو deamination موجود بنسبة 1%
+
+142
+00:09:21,680 --> 00:09:24,180
+Gelatin اللي هو قلنا البروتين اللي هتعمله دي
+
+143
+00:09:24,180 --> 00:09:27,900
+Amination H2S Indicator Ferric Ammonium Citrate
+
+144
+00:09:27,900 --> 00:09:30,720
+السبستريات لهذا الفحص Sodium Thiosulfate الـ BH
+
+145
+00:09:30,720 --> 00:09:35,080
+Indicator البروموس الذي هو البربول الأجاركاس والـ
+
+146
+00:09:35,080 --> 00:09:38,360
+Divying Agent الـ BH للميديا من غير ما أنا أزراحها
+
+147
+00:09:38,360 --> 00:09:42,340
+ستة أو سبعة نلاحظ أن لون الميديا في الأصل من قبل
+
+148
+00:09:42,340 --> 00:09:48,960
+ما أنا أزراحها بيكون لونها بنفسي فاتح هذه الميديا
+
+149
+00:09:48,960 --> 00:09:52,540
+بدي أكشف أنقدرة الـ Gram Negative plus الـ Lie
+
+150
+00:09:52,540 --> 00:09:56,800
+إنها تعمل D-Carboxylate او D-Aminate للـ Lysine هى
+
+151
+00:09:56,800 --> 00:10:00,120
+R مش And يبقى البكتيريا إما هتعمل D-Carboxylation
+
+152
+00:10:00,120 --> 00:10:03,860
+للـ Lysine أو هتعمل D-Amination ممنوع التنان
+
+153
+00:10:03,860 --> 00:10:07,320
+يكونوا مع بعض مش catably and R ممكن هذا أو هذا أو
+
+154
+00:10:07,320 --> 00:10:11,560
+ممكن ماهي تعمل للتنان أي ممكن ماهي تعمل D
+
+155
+00:10:11,560 --> 00:10:14,720
+-Carboxylation ولا D-Amination كمان بدي أكشف عن
+
+156
+00:10:14,720 --> 00:10:18,980
+قدرتها إنها تكون H2S productionبقول إن الليسين دي
+
+157
+00:10:18,980 --> 00:10:21,500
+ammunition بيصير قلنا في ال aerobic condition
+
+158
+00:10:21,500 --> 00:10:25,200
+فبالتالي هاكشف عنه في ال slant ال decarboxylation
+
+159
+00:10:25,200 --> 00:10:28,780
+بيصير في ظروف unaerobic فبالتالي هاكشف عنه في البط
+
+160
+00:10:28,780 --> 00:10:32,140
+السلمونيل أو ال citrobacter إذا ملاحظين التنتين
+
+161
+00:10:32,140 --> 00:10:36,140
+H2S positive يبقى السلمونيل اللي هيمرت معنا ال
+
+162
+00:10:36,140 --> 00:10:40,020
+brute yes H2S positive من الكوليفارم ال
+
+163
+00:10:40,020 --> 00:10:44,540
+citrobacter H2S positive إذا ملاحظ هان جنب ال H2S
+
+164
+00:10:44,540 --> 00:10:48,370
+فيالبط فيه اندهان alkaline و فيه اندهان acidic
+
+165
+00:10:48,370 --> 00:10:51,710
+اللي هو ال yellow في ال triple sugar iron agar
+
+166
+00:10:51,710 --> 00:10:55,470
+ممنوع اشوف هاي ممنوع اشوف ال alkaline جنب ال H2S
+
+167
+00:10:55,470 --> 00:10:59,690
+production لكن في ال lysine iron agar قولنا في حال
+
+168
+00:10:59,690 --> 00:11:03,930
+كان عندي H2S positive ماقدر احدد هل هي alkaline او
+
+169
+00:11:03,930 --> 00:11:08,230
+هل هي acidic اللي هو yellow هل هي عملت اللي هو
+
+170
+00:11:08,230 --> 00:11:12,290
+lysine decarboxylation او عملت gelicose
+
+171
+00:11:12,290 --> 00:11:17,480
+fermentation تمامهذه معناها أن البكتيريا عملت
+
+172
+00:11:17,480 --> 00:11:19,960
+جليكوز fermentation السلموني اللي عملت جليكوز
+
+173
+00:11:19,960 --> 00:11:23,080
+fermentation اتكون عندى acidic condition ال acidic
+
+174
+00:11:23,080 --> 00:11:26,600
+condition كان مهم جدا ل ال H2S production اتكون
+
+175
+00:11:26,600 --> 00:11:31,380
+عندى ال H2S gas وكان مهم جدا كمان ل ال lycine
+
+176
+00:11:31,380 --> 00:11:36,340
+decarboxylation فاتحول اللون بعدها للون ال bare
+
+177
+00:11:36,340 --> 00:11:40,580
+ball اللي هو ال alkaline لكن هان اتكون عندى عملت
+
+178
+00:11:40,580 --> 00:11:43,060
+البكتيريا جليكوز fermentation اتكون acidic
+
+179
+00:11:43,060 --> 00:11:48,410
+conditionكان مهم لإنتاج
+
+180
+00:11:48,410 --> 00:11:51,150
+الـ H2 acid production لكن مع ان البكتيريا كان
+
+181
+00:11:51,150 --> 00:11:55,610
+القدرة انها تعمل Lycine decarboxylation فعملت
+
+182
+00:11:55,610 --> 00:12:01,020
+Lycine اللي هو deaminationأو عملات ممكن كمان اللي
+
+183
+00:12:01,020 --> 00:12:08,200
+هي الـ G-Latin Deamination هذا بالنسبة لهذه
+
+184
+00:12:08,200 --> 00:12:11,360
+التفسير النتائج هنا ممكن احنا نشوف الـ triple
+
+185
+00:12:11,360 --> 00:12:15,080
+sugar iron agar alkaline أو acidic jump في الـ
+
+186
+00:12:15,080 --> 00:12:18,520
+butt jump الـ H2S gas لأنه احنا قلنا اللي يسمى D
+
+187
+00:12:18,520 --> 00:12:22,880
+-carboxylationبيصير في ظروف بتحفظ في ظروف acidic
+
+188
+00:12:22,880 --> 00:12:25,520
+condition فأي بكتيريا عاملة lysine decarboxylation
+
+189
+00:12:25,520 --> 00:12:28,540
+بالاصل هي بتكون عاملة جليكوز fermentation اتكون
+
+190
+00:12:28,540 --> 00:12:31,700
+acidic condition لازم لاقى الـ H2O acid production
+
+191
+00:12:31,700 --> 00:12:35,120
+و لازم لاقى ال lysine decarboxylation هلأ ال
+
+192
+00:12:35,120 --> 00:12:39,200
+lysine decarboxylation تمام بتكون في عندي مركب
+
+193
+00:12:39,200 --> 00:12:44,740
+قاعدي اللي هو ال cadaverin هذا هيرفعلي ال ال BH
+
+194
+00:12:44,740 --> 00:12:52,080
+وهيحول اللون الوسط للون بارفلكيف أنا بزرع اللي هو
+
+195
+00:12:52,080 --> 00:12:57,120
+بعمل stabbing للـBUT و بزرع اللي هو الـslant
+
+196
+00:12:57,120 --> 00:13:03,140
+زيجزاج هذا بالنسبة للنتاج احنا قلنا لو انا شوفت في
+
+197
+00:13:03,140 --> 00:13:08,020
+ال .. بشوف فيه اول اشي في الـBUT الـBUT لو كان في
+
+198
+00:13:08,020 --> 00:13:11,180
+عندي الـBUT purple يبقى بعرف انه و الله صار في
+
+199
+00:13:11,180 --> 00:13:14,740
+عندي lysine decarboxylation positive بما انه صار
+
+200
+00:13:14,740 --> 00:13:17,120
+في عندي lysine decarboxylation ده أكون عارفة في
+
+201
+00:13:17,120 --> 00:13:20,910
+الأصل عملة البكتيرياجليكوز fermentation لأن
+
+202
+00:13:20,910 --> 00:13:24,290
+البكتيريا عندها القدرة أن تعمل lysine
+
+203
+00:13:24,290 --> 00:13:28,630
+decarboxylation هذا بيصير في ظروف acidic condition
+
+204
+00:13:28,630 --> 00:13:31,810
+من وين بده ييجي من الجليكوز fermentation يبقى حد
+
+205
+00:13:31,810 --> 00:13:35,050
+ما أشوف في بيربول في ال بط بيربول يبقى lysine
+
+206
+00:13:35,050 --> 00:13:38,010
+decarboxylation جنبه على طول جليكوز fermentation
+
+207
+00:13:38,010 --> 00:13:44,510
+تمامبالنسبة لـ Slice Lent بما انه اتكون لون باربل
+
+208
+00:13:44,510 --> 00:13:47,650
+فيه لسلنت يبقى بدي اعرف انه البكتيريا عملت gelatin
+
+209
+00:13:47,650 --> 00:13:51,430
+deamination ممنوع انها تكون عاملة lysine
+
+210
+00:13:51,430 --> 00:13:53,590
+deamination لأنه بما انها عملت lysine
+
+211
+00:13:53,590 --> 00:13:56,630
+decarboxylation اكيد مش هتعمل lysine deamination
+
+212
+00:13:56,630 --> 00:14:02,270
+باربل و باربل قلنا الرمز هيكون K على K هن في البط
+
+213
+00:14:02,270 --> 00:14:06,760
+اكيد البكتيريا عملت glucose fermentationماشوفتش
+
+214
+00:14:06,760 --> 00:14:10,520
+اللون البربول في البط يبقى lysine decarboxylase
+
+215
+00:14:10,520 --> 00:14:15,420
+negative بالنسبة للبربول بالإسلان يبقى برضه كانت
+
+216
+00:14:15,420 --> 00:14:19,400
+lysine deamination negative يبقى هي عملت هان اللي
+
+217
+00:14:19,400 --> 00:14:23,880
+هو deamination للجيلاتين يبقى هان البكتيريا
+
+218
+00:14:23,880 --> 00:14:27,180
+ماعندها القدرة إنها تعمل ولا deamination للليسين
+
+219
+00:14:27,180 --> 00:14:31,120
+ولا decarboxylation للليسين بما إن يالو يبقى الرمز
+
+220
+00:14:31,120 --> 00:14:35,260
+A بما إن بيربول يبقى الرمز KYellow في البط يبقى
+
+221
+00:14:35,260 --> 00:14:37,840
+Gelicose fermentation positive Lycine
+
+222
+00:14:37,840 --> 00:14:42,160
+decarboxylation negative بالنسبة للإسلاند ماعندي
+
+223
+00:14:42,160 --> 00:14:46,940
+لون purple يبقى ما اتكون عندي اللي هو الجيلاتين
+
+224
+00:14:46,940 --> 00:14:52,260
+اللي بالنسبة للإسلاند اللون ال red معناه اللي هو
+
+225
+00:14:52,260 --> 00:14:56,740
+Lycine deamination positive لكن ماعملت جيلاتين
+
+226
+00:14:56,740 --> 00:14:59,600
+deamination يبقى هان اللون ال red في الإسلاند
+
+227
+00:14:59,600 --> 00:15:03,850
+معناه Lycine deamination positive يبقى Yellow Aالـ
+
+228
+00:15:03,850 --> 00:15:07,470
+Red قلنا بما أن الـ Red بقى برمز لإله بالرمز R مش
+
+229
+00:15:07,470 --> 00:15:11,750
+الرمز K هاي البكتيريا فقط عندها القدرة أنها تعمل
+
+230
+00:15:11,750 --> 00:15:14,690
+Deamination للليسينومينة عندها القدرة أنها تعمل
+
+231
+00:15:14,690 --> 00:15:18,510
+Decarboxylation للليسينومينة الـ Black precipitate
+
+232
+00:15:18,510 --> 00:15:22,710
+قلنا الـ H2S production هاي ما بقدر أشوفها أو ما
+
+233
+00:15:22,710 --> 00:15:28,010
+بقدر إذا كانت H2S production أحدد هل هي A أو هل هي
+
+234
+00:15:28,010 --> 00:15:31,700
+K هل هي Gelicose fermentation أو هل هياللي هي
+
+235
+00:15:31,700 --> 00:15:34,920
+lysine decarboxylation مع gelicose fermentation
+
+236
+00:15:34,920 --> 00:15:39,280
+لازم أعمل الفحص لحالي لازم أعمل media يكون مافيها
+
+237
+00:15:39,280 --> 00:15:44,600
+ال substrate اللي هو لل sodium thiosulfate
+
+238
+00:15:44,600 --> 00:15:50,880
+reductase طيب في هذه ال media ممنوع اشوف ممنوع
+
+239
+00:15:50,880 --> 00:15:54,580
+احنا نشوف انه النتيجة يكون في عندي lysine
+
+240
+00:15:54,580 --> 00:15:57,960
+deamination تمام lysine deamination في الإسلات
+
+241
+00:15:57,960 --> 00:16:01,710
+ممنوع اشوف اللون ال redفي الـ slant واللون ال
+
+242
+00:16:01,710 --> 00:16:06,630
+berbol في البط لأن اللون ال red في ال slant معناه
+
+243
+00:16:06,630 --> 00:16:10,830
+lycindy amination positive اللون ال berbol في البط
+
+244
+00:16:10,830 --> 00:16:13,610
+معناه lycindy carboxylation positive وإحنا قلنا
+
+245
+00:16:13,610 --> 00:16:16,510
+ممنوع البكتيريا تعمل الأثنان مع بعض يبقى اللون ال
+
+246
+00:16:16,510 --> 00:16:19,170
+red في ال slant و ال berbol بالبط ممنوع أني أنا
+
+247
+00:16:19,170 --> 00:16:27,380
+أشوفههذه تفسير النتائج هنا نلاحظ أن اللون purple
+
+248
+00:16:27,380 --> 00:16:31,880
+في الإسلاند و purple في البط يبقى K على K طبعا
+
+249
+00:16:31,880 --> 00:16:35,200
+تفسير النتائج زي ما قلنا بما أن purple و purple
+
+250
+00:16:35,200 --> 00:16:39,720
+purple في الإسلاند يبقى gelatin purple في الإسلاند
+
+251
+00:16:39,720 --> 00:16:45,900
+يبقى gelatin deamination purple في البط
+
+252
+00:16:45,900 --> 00:16:51,590
+يبقى lysine decarboxylation هنا K على Aمنلاحظ أنه
+
+253
+00:16:51,590 --> 00:16:54,790
+هنا بما أنه في الـBot A يبقى Gelicose fermentation
+
+254
+00:16:54,790 --> 00:17:01,830
+في الـslant هنا K يبقى هي في الـslant تتحول للون
+
+255
+00:17:01,830 --> 00:17:04,950
+للون Violet يبقى هي Gelatin Deamination يبقى هنا
+
+256
+00:17:04,950 --> 00:17:07,190
+البكتيريا ماعندها القدرة أن تتعمل للليسين ولا
+
+257
+00:17:07,190 --> 00:17:11,950
+Deamination ولا Decarboxylation هنا الرمز في عندي
+
+258
+00:17:11,950 --> 00:17:14,610
+Yellow في الـBot يبقى Gelicose fermentation
+
+259
+00:17:14,610 --> 00:17:17,410
+positive ليسين Decarboxylation negative في عندى
+
+260
+00:17:17,410 --> 00:17:23,280
+تشققات ممكن نضيف Gasفي عندي هان لون Red في ال ..
+
+261
+00:17:23,280 --> 00:17:25,900
+اللي هو في الـ Slant يبقى عاملة الـ Bacteria
+
+262
+00:17:25,900 --> 00:17:33,360
+Lysine D Amination هان في عندي H2S اللي هو H2S
+
+263
+00:17:33,360 --> 00:17:37,680
+لأنه اللي اتكون اللون الأسود الـ Plus هاي معناها
+
+264
+00:17:37,680 --> 00:17:41,200
+أنه في عندي تشققات نسينا نقول في ال triple sugar
+
+265
+00:17:41,200 --> 00:17:44,700
+iron agar ممكن أضيف رمز الجاز و ممكن أضيف رمز الـ
+
+266
+00:17:44,700 --> 00:17:47,880
+Plus معناه أنه والله في عندي اتكون جاز يبقى رمز
+
+267
+00:17:47,880 --> 00:17:51,950
+الـ Plusمعناه في عندي اللي هو اتكون نتيجة
+
+268
+00:17:51,950 --> 00:17:55,950
+fermentation acid with gas يبقى ال plus ممكن ارمز
+
+269
+00:17:55,950 --> 00:18:00,390
+ال plus ممكن ارمز اللي هو ال gas طيب فوق في ال
+
+270
+00:18:00,390 --> 00:18:04,530
+slant اتكون اللون اللي هو ال red يبقى صار في عندي
+
+271
+00:18:04,530 --> 00:18:10,370
+اللي هو lysine deamination طيب ليش أنا كتبت ايه؟
+
+272
+00:18:10,370 --> 00:18:13,930
+ليش أنا كتبت ايه؟ احنا قلنا في هذه الحالة بما انه
+
+273
+00:18:13,930 --> 00:18:20,320
+اتكون H2S gas ماقدر اكتب ايه ولا كامهان بقوللي في
+
+274
+00:18:20,320 --> 00:18:24,320
+هذه الحالة بما أن البكتيريا عملت ليسين D-amination
+
+275
+00:18:24,320 --> 00:18:27,500
+كانت أعرفه أكيد مش هتكون عاملة ليسين D
+
+276
+00:18:27,500 --> 00:18:31,540
+-carboxylation فبالتالي برمز للرمز A يبقى في هذه
+
+277
+00:18:31,540 --> 00:18:34,780
+الحالة انا احنا قولنا الـH2S بما أنه اتكون في الـ
+
+278
+00:18:34,780 --> 00:18:39,280
+triple في الـ lycine iron agar يبقى ما بقدر أحدد A
+
+279
+00:18:39,280 --> 00:18:43,640
+أو K لأن الـH2S production بيصير في ظروف acidic
+
+280
+00:18:43,640 --> 00:18:46,620
+condition الـA فعلا صار في انديجليكوز fermentation
+
+281
+00:18:47,230 --> 00:18:49,710
+بالنسبة للـ K في الأصل هي عملت جليكوز fermentation
+
+282
+00:18:49,710 --> 00:18:53,550
+لما بعدين عملت lysine decarboxylation لكن هنا أنا
+
+283
+00:18:53,550 --> 00:18:58,990
+كتبت A لأن الرمز الـ R اللي هو الـ R مع أنها صار
+
+284
+00:18:58,990 --> 00:19:01,650
+عندي lysine deamination لما أنها عملت lysine
+
+285
+00:19:01,650 --> 00:19:04,670
+deamination يبقى مع عملت lysine decarboxylation
+
+286
+00:19:04,670 --> 00:19:07,970
+يبقى فقط بيكون عندي اللي تحت اللي هو الجليكوز
+
+287
+00:19:07,970 --> 00:19:11,270
+fermentation هاي برضه منضيف اللي هو الـ plus R
+
+288
+00:19:11,270 --> 00:19:16,200
+الجاز عليها لأنه في عندي تشققات تحتخلصنا هيك
+
+289
+00:19:16,200 --> 00:19:20,040
+ميديات اللي هي Lycine Iron Agarni جلال الميديا
+
+290
+00:19:20,040 --> 00:19:24,280
+التانية اللي هي ال Motility Indole Ornithine MIO
+
+291
+00:19:24,280 --> 00:19:29,340
+بما أن في عندي Motility يبقى هتكون Stab at you هذه
+
+292
+00:19:29,340 --> 00:19:33,300
+الميديا مش هاكشف على ال H2S Gas هتوفر لي European
+
+293
+00:19:33,300 --> 00:19:36,860
+Condition فا من خلالها هاكشف على ال Indole وعلى ال
+
+294
+00:19:36,860 --> 00:19:39,660
+Ornithine Deamination أي ال Deamination بيصير في
+
+295
+00:19:39,660 --> 00:19:42,830
+ظروف European Conditionبالنسبة للـ Un-Aerobic
+
+296
+00:19:42,830 --> 00:19:45,030
+Condition قلنا الجليكوز fermentation والـ
+
+297
+00:19:45,030 --> 00:19:48,170
+decarboxylation بيصير في الـ Un-Aerobic Condition
+
+298
+00:19:48,170 --> 00:19:52,510
+الـ pH Indicator نفس ميديا الليسين ايرون اجاري
+
+299
+00:19:52,510 --> 00:19:58,490
+اللي هي البراموس رزال بيربال بتكون لون يلو في ال
+
+300
+00:19:58,490 --> 00:20:01,430
+acid condition في ال basil condition بتكون لون
+
+301
+00:20:01,430 --> 00:20:05,610
+بيربال فيه لنيوترال بيكون لون إجريكطيب احنا قلنا
+
+302
+00:20:05,610 --> 00:20:10,370
+في تحت لو انا شوفت لون أصفر معناه انه البكتيريا
+
+303
+00:20:10,370 --> 00:20:14,250
+عملت gelicose fermentation اتكوّن ال acid حوّل لون
+
+304
+00:20:14,250 --> 00:20:19,010
+ال ال بي اتش indicator لللون اليالو طب لو انا شوفت
+
+305
+00:20:19,010 --> 00:20:22,030
+تحت لون bearable معناه انه البكتيريا عملت
+
+306
+00:20:22,030 --> 00:20:26,250
+decarboxylation لأرنيثين اتكوّن alkaline product و
+
+307
+00:20:26,250 --> 00:20:30,650
+حولت ال media لللون bearable طب على السطح لو انا
+
+308
+00:20:30,650 --> 00:20:34,420
+ضفت ال cavex reagent يبقىالبكتيريا عندها أنزيم
+
+309
+00:20:34,420 --> 00:20:38,000
+لتريبتوفاناز جسرت لتريبتوفان اتكون اندول ارتبط مع
+
+310
+00:20:38,000 --> 00:20:42,500
+الكوفكريازيران تتكون اندي red ring طب لو كان على
+
+311
+00:20:42,500 --> 00:20:45,120
+سطح لون فيه purple معناه ان البكتيريا عملت
+
+312
+00:20:45,120 --> 00:20:48,700
+deamination لارنيثين اتكون اندي alkaline product
+
+313
+00:20:48,700 --> 00:20:52,840
+حول ال pH indicator لللون ال purple هذا بالنسبة
+
+314
+00:20:52,840 --> 00:20:57,870
+لهاي ال mediaنشوف مكونات ال media أكيد yes extract
+
+315
+00:20:57,870 --> 00:21:04,090
+كمصادر للتغذية بيبتون تمام لأن البكتيري هتعمل ال
+
+316
+00:21:04,090 --> 00:21:11,110
+ال deamination للبيبتون sorry في هذه الفقرة نمسح
+
+317
+00:21:11,110 --> 00:21:16,270
+ال ornithine deamination و نكتب بيبتون deamination
+
+318
+00:21:16,270 --> 00:21:20,830
+هان
+
+319
+00:21:20,830 --> 00:21:23,710
+بيبتون deamination مش decarboxylation
+
+320
+00:21:26,080 --> 00:21:29,500
+مش ornithine هان اللي هي ال peptone دي amination و
+
+321
+00:21:29,500 --> 00:21:33,080
+هان اللي هي برضه ال peptone دي amination تمام؟ بدي
+
+322
+00:21:33,080 --> 00:21:36,100
+أكشف في هذه ال media عن قدرة البكتيريا أنها تعمل
+
+323
+00:21:36,100 --> 00:21:38,680
+decarboxylation لل ornithine لو ماعملت
+
+324
+00:21:38,680 --> 00:21:42,120
+decarboxylation هتعمل deamination لل peptone في ال
+
+325
+00:21:42,120 --> 00:21:42,960
+European condition
+
+326
+00:21:46,330 --> 00:21:48,710
+بالنسبة لأ ال Ornithin موجود أكيد في ال media
+
+327
+00:21:48,710 --> 00:21:51,430
+لأنها أكشف عن قدرة البكتيريا أن تعمل دي
+
+328
+00:21:51,430 --> 00:21:54,650
+Carboxylation ال Dextrose موجود بكمية قليلة عشان
+
+329
+00:21:54,650 --> 00:21:58,410
+أكشف على ال fermentation الأجار بما أن فحص
+
+330
+00:21:58,410 --> 00:22:02,350
+Motility يبقى هي semi-solid media نسبة الأجار 4 من
+
+331
+00:22:02,350 --> 00:22:08,230
+10% ال pH indicator ال برامو سرزال بيربول قلنا
+
+332
+00:22:08,230 --> 00:22:13,020
+حدوده شو بتكون في ال acidic و ال basicالـ MIO
+
+333
+00:22:13,020 --> 00:22:18,880
+media تستخدم عشان اني انا افرق اللي هو ال او اكشف
+
+334
+00:22:18,880 --> 00:22:22,740
+على تلت فحوصات اللي هي في خلال one culture two
+
+335
+00:22:22,740 --> 00:22:25,920
+motility in dolly production and ornizine
+
+336
+00:22:25,920 --> 00:22:29,250
+decarboxylationبالنسبة لـ Motility قلنا بعمل
+
+337
+00:22:29,250 --> 00:22:33,390
+Stabbing في الـ Semi Solid Media باستخدام الـ
+
+338
+00:22:33,390 --> 00:22:36,750
+Straight Wire من خلال اللي هو الـ Inculating
+
+339
+00:22:36,750 --> 00:22:42,010
+Needle لو البكتيريا Motile بقولي أكيد ه will
+
+340
+00:22:42,010 --> 00:22:45,430
+migrate from the stab line by means of their
+
+341
+00:22:45,430 --> 00:22:49,610
+flagella البكتيريا هتتحرك بعيدا عن حط الزراعة
+
+342
+00:22:49,610 --> 00:22:52,890
+باستخدام الفلاجلة producing turbidity or
+
+343
+00:22:52,890 --> 00:22:54,950
+cloudiness through other media هيتكون عندك
+
+344
+00:22:54,950 --> 00:22:58,320
+turbidity و cloudiness في الميدياالـ Non-motile
+
+345
+00:22:58,320 --> 00:23:03,180
+organism هتنمو فقط على خط الزراعة فبالتالي باقي ال
+
+346
+00:23:03,180 --> 00:23:07,940
+media هتكون clear بالنسبة للفحص التاني اللي هو ال
+
+347
+00:23:07,940 --> 00:23:12,460
+Ornithin بقولي هاي ال media بدي أكشف على وجود
+
+348
+00:23:12,460 --> 00:23:15,800
+إنزيم ال Ornithin decarboxylase هل البكتيريا عندها
+
+349
+00:23:15,800 --> 00:23:20,280
+القدرة أنها تعمل decarboxylase ل Ornithin ولا لأ
+
+350
+00:23:20,280 --> 00:23:24,730
+باستخدام اللي هو ال Ornithin الموجود في ال mediaلو
+
+351
+00:23:24,730 --> 00:23:27,690
+البكتيريا عندها هذا الانزيم بقوللي هذا الانزيم
+
+352
+00:23:27,690 --> 00:23:31,450
+الارنيسين دي كربوكسيلاز نفس اللايسين دي كربوكسيلاز
+
+353
+00:23:31,450 --> 00:23:35,830
+بتنشط في ال acidic environment زي ما قلنا create
+
+354
+00:23:35,830 --> 00:23:39,570
+by the initial fermentation of the glycosic من وين
+
+355
+00:23:39,570 --> 00:23:43,310
+بده يجي ال acidic environment اللي هو ال
+
+356
+00:23:43,310 --> 00:23:46,490
+environment ال acidic information او ال acidic
+
+357
+00:23:46,490 --> 00:23:49,710
+اللي هو ال acidic condition او ال acidic
+
+358
+00:23:49,710 --> 00:23:54,030
+environment بقوللي ال acidic environment بيكونصير
+
+359
+00:23:54,030 --> 00:23:56,870
+في الأصل من خلال أن البكتيريا بالأول بتعمل
+
+360
+00:23:56,870 --> 00:24:01,050
+fermentation للجليكوز تكون عندى acidic condition
+
+361
+00:24:01,050 --> 00:24:06,110
+فبالتالي هيتحفظ لإنتاج اللى هو او لعمل ال
+
+362
+00:24:06,110 --> 00:24:09,510
+decarboxylation للأورنيكين نفس فكرة ال lycine
+
+363
+00:24:09,510 --> 00:24:13,650
+decarboxylase قولنا بيصير في acidic condition عشان
+
+364
+00:24:13,650 --> 00:24:16,830
+هيك ممكن اني انا اشوف ال H2O acid protection جنبه
+
+365
+00:24:16,830 --> 00:24:21,390
+alkaline او acidic بقولى ال amino acid لو صلوا
+
+366
+00:24:21,390 --> 00:24:22,510
+decarboxylated
+
+367
+00:24:26,500 --> 00:24:30,020
+البرودكت الناتج اللى هو البرودكت الناتج الهو
+
+368
+00:24:30,020 --> 00:24:32,520
+البرودكت الناتج الهو البرودكت الناتج الهو البرودكت
+
+369
+00:24:32,520 --> 00:24:33,020
+الناتج الهو البرودكت الناتج الهو البرودكت الناتج
+
+370
+00:24:33,020 --> 00:24:35,520
+الهو البرودكت الناتج الهو البرودكت الناتج الهو
+
+371
+00:24:35,520 --> 00:24:39,060
+البرودكت الناتج الهو البرودكت الناتج الهو
+
+372
+00:24:39,060 --> 00:24:42,680
+البرودكت
+
+373
+00:24:42,680 --> 00:24:43,500
+الناتج الهو البرودكت الناتج الهو البرودكت الناتج
+
+374
+00:24:43,500 --> 00:24:43,660
+البرودكت الناتج الهو البرودكت الناتج الهو البرودكت
+
+375
+00:24:43,660 --> 00:24:48,880
+الناتفي Lysine Decarboxylase في ميديا Lysine Iron
+
+376
+00:24:48,880 --> 00:24:53,580
+Agar كان المركب الناتج من ال Lysine Decarboxylase
+
+377
+00:24:53,580 --> 00:24:57,620
+الكفاديرين تمام لكن هان هذا هو المركب الناتج من
+
+378
+00:24:57,620 --> 00:25:02,670
+عملية ال ال Ornithine Decarboxylationبقول لي هيرفع
+
+379
+00:25:02,670 --> 00:25:05,650
+الـ pH هيتكوّن عندي alkaline product فبالتالي
+
+380
+00:25:05,650 --> 00:25:09,690
+هيتحول لون ال media للون dark purple لو البكتيريا
+
+381
+00:25:09,690 --> 00:25:11,930
+ما عندها القدرة أو ما عندها ال enzyme في الأصل
+
+382
+00:25:11,930 --> 00:25:16,710
+فبالتالي هيظل أسدك الناتج من عملية ال fermentation
+
+383
+00:25:16,710 --> 00:25:20,970
+لجليكوز فهيتحول لون ال media في اللي هو البطم في
+
+384
+00:25:20,970 --> 00:25:22,750
+تحت هيتحول للون أصفر
+
+385
+00:25:27,250 --> 00:25:30,130
+أخر اشي فحص الاندول باكشف عن وجود اللي هو الـ
+
+386
+00:25:30,130 --> 00:25:33,670
+tryptophan الـ tryptophan موجود في الميديا في اللي
+
+387
+00:25:33,670 --> 00:25:38,510
+هو ال بيبتون تمام بالنسبة لل positive result
+
+388
+00:25:38,510 --> 00:25:43,630
+هيتحول لون اللي هو في ال على السطح red color اللي
+
+389
+00:25:43,630 --> 00:25:46,910
+هو ال cherry red color هذا معناه أن البكتيريا
+
+390
+00:25:46,910 --> 00:25:49,530
+عندها أنزيم لـ tryptophan بعد إضافة اللي هو
+
+391
+00:25:49,530 --> 00:25:53,130
+الكوفكس رياجان رياجان لكن ال negative result مش
+
+392
+00:25:53,130 --> 00:26:00,430
+هيتغير أي لونطب نيجي للنتائج بس
+
+393
+00:26:00,430 --> 00:26:07,190
+بالنسبة للنتائج ها يندي تلت نتائج لكن طبعا هن واحد
+
+394
+00:26:07,190 --> 00:26:10,230
+و واحد هي نفس الشيء لكن هن بعد إضافة ال govx
+
+395
+00:26:10,230 --> 00:26:16,370
+reagent هن اتنين و هن تلت نيجي بدنا أول شئ اللي هو
+
+396
+00:26:16,370 --> 00:26:21,870
+نكشف أو نقرأ نتيجة فحص ال motility تمام بعدين هقرأ
+
+397
+00:26:21,870 --> 00:26:27,490
+نتيجة الالـ Endol أو الـ Decarboxylase اللي هو الـ
+
+398
+00:26:27,490 --> 00:26:30,810
+Ornithine Decarboxylase بعدين هقرأ اللي هو هضيف ال
+
+399
+00:26:30,810 --> 00:26:35,410
+Kovacs reagent و هقرأ نتيجة اللي هو ال Endol test
+
+400
+00:26:35,410 --> 00:26:43,190
+بالنسبة لأول اللي هو ال tube أول tube بالنسبة لفحص
+
+401
+00:26:43,190 --> 00:26:47,990
+ال motility اللي هي non motile بنلاحظ أنه على خط
+
+402
+00:26:47,990 --> 00:26:54,440
+الزراعة بالنسبة لل Ornithine Decarboxylase قلناتحت
+
+403
+00:26:54,440 --> 00:27:00,020
+اتكون في على الاسفل اللي هو في ال in the bottom في
+
+404
+00:27:00,020 --> 00:27:03,640
+اللي هو الان ال condition صار في عندي لون أصفر
+
+405
+00:27:03,640 --> 00:27:07,000
+يبقى معناه جيليكوز fermentation positive ما صار في
+
+406
+00:27:07,000 --> 00:27:11,920
+عندي ornithine اللي هو دي كربوكسيلاز كانت negative
+
+407
+00:27:11,920 --> 00:27:15,180
+يبقى high ornithine دي كربوكسيلاز negative جيليكوز
+
+408
+00:27:15,180 --> 00:27:21,380
+fermentation positiveهنا بعد ما اضفت الكوفاكس
+
+409
+00:27:21,380 --> 00:27:26,420
+رياجان تحولت اللون الحلقة للون أحمر يبقى اندال
+
+410
+00:27:26,420 --> 00:27:33,200
+بوزيتف هنا في ND ما عملت البكتيريا ديكاربوكسلاشن
+
+411
+00:27:33,200 --> 00:27:37,920
+لأرنسين عملت ديأميناشن للبيبطون عشان هيك اتحول
+
+412
+00:27:37,920 --> 00:27:42,560
+اللون اللي على السطح للون violet للون بيربول dark
+
+413
+00:27:42,560 --> 00:27:47,330
+purpleبعد إضافة الـ Covax reagent نلاحظ أن اللون
+
+414
+00:27:47,330 --> 00:27:52,070
+البربل على السطح فيه اختفى لماذا؟ لأن الـ Covax
+
+415
+00:27:52,070 --> 00:27:56,690
+reagent أحد مكوناته الـ HCl إضافة الـ HCl يتحول
+
+416
+00:27:56,690 --> 00:28:00,230
+لون الـ media للون بما أن في عندي BH Indicator
+
+417
+00:28:00,230 --> 00:28:04,910
+لبرومو سيرزال بيربل موجود في ال acid condition لون
+
+418
+00:28:04,910 --> 00:28:10,310
+يلو فبالتالي اتحول للون يلو على السطحتغير اللان
+
+419
+00:28:10,310 --> 00:28:13,630
+اللى على السطح لللان يلو بعد إضافة الـ COVAX
+
+420
+00:28:13,630 --> 00:28:18,010
+reagent اتحول اللان للان يلو لأن الـ SCL عندى
+
+421
+00:28:18,010 --> 00:28:24,110
+acidic فبالتالي اتحول اللان للان يلو طيب نيجى لهاي
+
+422
+00:28:24,110 --> 00:28:29,970
+ال media أو لتانى فحص نيجى اللى اللى هو في البطام
+
+423
+00:28:29,970 --> 00:28:34,910
+بنحكى ornithine decarboxylase positive بما أنه صار
+
+424
+00:28:34,910 --> 00:28:38,200
+في عندي ornithine decarboxylasePositive يبقى أكيد
+
+425
+00:28:38,200 --> 00:28:41,160
+في الأصل كانت عندي جليكوز fermentation positive
+
+426
+00:28:41,160 --> 00:28:44,180
+يبقى هي جليكوز fermentation positive Ornithine
+
+427
+00:28:44,180 --> 00:28:51,140
+decarboxylase positive تمام بالنسبة لاللي هو ال
+
+428
+00:28:51,140 --> 00:28:54,780
+motility ال motility ماقدر أكشف على ال motility زي
+
+429
+00:28:54,780 --> 00:28:59,020
+بحث ال H2S production أحنا قلنا في حاجة عن عندي
+
+430
+00:28:59,020 --> 00:29:02,020
+H2S production ماقدر أكشف على ال motility في ميدية
+
+431
+00:29:02,020 --> 00:29:06,040
+ال SIM وهنا نفس الفكرة ماقدر أكشف على ال motility
+
+432
+00:29:06,040 --> 00:29:11,910
+في ميديةالـ MIO في حال كانت عملة البكتيريا أو الـ
+
+433
+00:29:11,910 --> 00:29:16,150
+Nitin-D-Carboxylase لازم أعمل الفحص لحال في ميديا
+
+434
+00:29:16,150 --> 00:29:20,550
+الـ Nutrient Growth مع أجار بنسبة 4 من 10% تمام
+
+435
+00:29:20,550 --> 00:29:24,730
+هذا بالنسبة لهذه الميديا ضيفت اللي هو الـ COVAX
+
+436
+00:29:24,730 --> 00:29:28,610
+-Reagent يبقى الـ Indole Negative ملاحظ إنه اللون
+
+437
+00:29:28,610 --> 00:29:31,630
+صار لونه أصفر لأن أحنا قلنا الـ COVAX-Reagent في
+
+438
+00:29:31,630 --> 00:29:37,540
+HCL هذه الميديا الـ Motility بنكتب unknownوها نفس
+
+439
+00:29:37,540 --> 00:29:39,620
+الشيء نكتب الـ motility unknown ornithine
+
+440
+00:29:39,620 --> 00:29:42,640
+decarboxylase positive بما أنه عملت decarboxylase
+
+441
+00:29:42,640 --> 00:29:45,620
+لالورنثين يبقى هي جليكوز في الأصل fermentation
+
+442
+00:29:45,620 --> 00:29:52,280
+positive ضيفت ال Kovacs reaction اتكون عندي حلقة
+
+443
+00:29:52,280 --> 00:29:56,600
+حمرة يبقى هي indole positive يبقى ال motility
+
+444
+00:29:56,600 --> 00:29:59,900
+unknown ornithine decarboxylase positive بما أنه
+
+445
+00:29:59,900 --> 00:30:01,920
+عملت هاك يبقى هي في الأصل جليكوز fermentation
+
+446
+00:30:01,920 --> 00:30:05,100
+positive لأنه احنا قلنا ال decarboxylation بيصير
+
+447
+00:30:05,100 --> 00:30:08,770
+في acidic environmentالـ Indol بما أنه اتحول أو
+
+448
+00:30:08,770 --> 00:30:12,170
+اتكون حلقة حمرا يبقى هي positive ليش تغير لونها
+
+449
+00:30:12,170 --> 00:30:15,310
+للون أصفر نفس الفكرة الـ COVAX-REACTION بتكون من
+
+450
+00:30:15,310 --> 00:30:20,510
+HCl هيتحول الوسط لوسط حمضي و هيحول لون الـ pH
+
+451
+00:30:20,510 --> 00:30:25,470
+Indicator للون Yellow هيك بنكون خلصنا اللي هو
+
+452
+00:30:25,470 --> 00:30:29,730
+Chapter Single Media Multiple Test يعطيكم العافية
+
+453
+00:30:29,730 --> 00:30:31,990
+والسلام عليكم ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/_HOd9t_eBH4_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/_HOd9t_eBH4_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..0d59d8d4f8e1c91d326bbad6534a797132143b81
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/_HOd9t_eBH4_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 3095, "start": 1.89, "end": 30.95, "text": "من الأمثلة الأخرى على single media multiple test ليسين آيرون أجار ليسين آيرون أجار من اسمها أكيد في الميديا في عندي ليسين الليسين يعتبر amino acid في الميديا هى بدي أكشف هل البكتيريا بتعمل deamination للليسين او decarboxylation للليسين آيرون بما أنه في آيرون في اسم الميديا يبقى في عندي H2S production بدي أكشف على الـH2S أجار يبقى", "tokens": [27842, 16247, 2304, 12984, 37977, 16247, 34740, 7578, 15844, 2167, 3021, 3866, 1500, 32239, 3794, 9957, 19753, 13546, 11536, 5551, 7435, 9640, 32239, 3794, 9957, 19753, 13546, 11536, 5551, 7435, 9640, 9154, 24525, 2304, 11296, 5551, 4117, 25708, 8978, 9673, 1829, 16254, 995, 8978, 18871, 16254, 32239, 3794, 9957, 13672, 1829, 3794, 9957, 7251, 34268, 26890, 24674, 8258, 8978, 9673, 1829, 16254, 995, 8032, 7578, 4724, 16254, 5551, 4117, 8592, 5172, 8032, 1211, 29739, 4117, 2655, 13546, 25528, 39894, 25957, 1211, 368, 335, 2486, 24976, 20292, 3794, 9957, 1975, 2407, 979, 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ممكن", "probability": 0.99267578125}, {"start": 610.92, "end": 611.1, "word": " ماهي", "probability": 0.9539388020833334}, {"start": 611.1, "end": 611.4, "word": " تعمل", "probability": 0.9915364583333334}, {"start": 611.4, "end": 611.56, "word": " D", "probability": 0.7587890625}, {"start": 611.56, "end": 612.28, "word": "-Carboxylation", "probability": 0.97451171875}, {"start": 612.28, "end": 612.54, "word": " ولا", "probability": 0.77294921875}, {"start": 612.54, "end": 612.72, "word": " D", "probability": 0.98193359375}, {"start": 612.72, "end": 613.3, "word": "-Amination", "probability": 0.9783935546875}, {"start": 613.3, "end": 614.02, "word": " كمان", "probability": 0.9842122395833334}, {"start": 614.02, "end": 614.24, "word": " بدي", "probability": 0.900634765625}, {"start": 614.24, "end": 614.56, "word": " أكشف", "probability": 0.9705810546875}, {"start": 614.56, "end": 614.72, "word": " عن", "probability": 0.94580078125}, {"start": 614.72, "end": 615.16, "word": " قدرتها", "probability": 0.9825439453125}, {"start": 615.16, "end": 615.36, "word": " إنها", "probability": 0.91064453125}, {"start": 615.36, "end": 615.68, "word": " تكون", "probability": 0.914794921875}, {"start": 615.68, "end": 616.54, "word": " H2S", "probability": 0.9264322916666666}, {"start": 616.54, "end": 617.14, "word": " production", "probability": 0.51416015625}], "temperature": 1.0}, {"id": 24, "seek": 64482, "start": 617.84, "end": 644.82, "text": "بقول إن الليسين دي ammunition بيصير قلنا في ال aerobic condition فبالتالي هاكشف عنه في ال slant ال decarboxylation بيصير في ظروف unaerobic فبالتالي هاكشف عنه في البط السلمونيل أو ال citrobacter إذا ملاحظين التنتين H2S positive يبقى السلمونيل اللي هيمرت معنا ال brute yes H2S positive من الكوليفارم ال citrobacter H2S positive إذا ملاحظ هان جنب ال H2S في", "tokens": [3555, 39648, 36145, 13672, 1829, 3794, 9957, 11778, 1829, 32251, 4724, 1829, 9381, 13546, 12174, 1211, 8315, 8978, 2423, 257, 2032, 65, 299, 4188, 6156, 3555, 6027, 2655, 6027, 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628.08, "end": 628.3, "word": " في", "probability": 0.9814453125}, {"start": 628.3, "end": 628.78, "word": " البط", "probability": 0.75927734375}, {"start": 628.78, "end": 630.06, "word": " السلمونيل", "probability": 0.58966064453125}, {"start": 630.06, "end": 630.22, "word": " أو", "probability": 0.54638671875}, {"start": 630.22, "end": 630.32, "word": " ال", "probability": 0.68115234375}, {"start": 630.32, "end": 630.86, "word": " citrobacter", "probability": 0.7294921875}, {"start": 630.86, "end": 631.08, "word": " إذا", "probability": 0.912109375}, {"start": 631.08, "end": 631.52, "word": " ملاحظين", "probability": 0.96201171875}, {"start": 631.52, "end": 632.14, "word": " التنتين", "probability": 0.841796875}, {"start": 632.14, "end": 633.1, "word": " H2S", "probability": 0.91455078125}, {"start": 633.1, "end": 633.52, "word": " positive", "probability": 0.69677734375}, {"start": 633.52, "end": 633.84, "word": " يبقى", "probability": 0.9383544921875}, {"start": 633.84, "end": 634.36, "word": " السلمونيل", "probability": 0.978515625}, {"start": 634.36, "end": 634.48, "word": " اللي", "probability": 0.925537109375}, {"start": 634.48, "end": 634.96, "word": " هيمرت", "probability": 0.6925455729166666}, {"start": 634.96, "end": 635.36, "word": " معنا", "probability": 0.889404296875}, {"start": 635.36, "end": 636.14, "word": " ال", "probability": 0.599609375}, {"start": 636.14, "end": 636.5, "word": " brute", "probability": 0.1915283203125}, {"start": 636.5, "end": 636.8, "word": " yes", "probability": 0.6201171875}, {"start": 636.8, "end": 637.5, "word": " H2S", "probability": 0.953125}, {"start": 637.5, "end": 638.04, "word": " positive", "probability": 0.80859375}, {"start": 638.04, "end": 638.7, "word": " من", "probability": 0.98486328125}, {"start": 638.7, "end": 639.92, "word": " الكوليفارم", "probability": 0.7110107421875}, {"start": 639.92, "end": 640.02, "word": " ال", "probability": 0.72119140625}, {"start": 640.02, "end": 640.76, "word": " citrobacter", "probability": 0.9192708333333334}, {"start": 640.76, "end": 641.54, "word": " H2S", "probability": 0.9830729166666666}, {"start": 641.54, "end": 641.94, "word": " positive", "probability": 0.89892578125}, {"start": 641.94, "end": 642.22, "word": " إذا", "probability": 0.97265625}, {"start": 642.22, "end": 643.06, "word": " ملاحظ", "probability": 0.965087890625}, {"start": 643.06, "end": 643.42, "word": " هان", "probability": 0.6226806640625}, {"start": 643.42, "end": 643.68, "word": " جنب", "probability": 0.96142578125}, {"start": 643.68, "end": 643.8, "word": " ال", "probability": 0.97607421875}, {"start": 643.8, "end": 644.54, "word": " H2S", "probability": 0.96923828125}, {"start": 644.54, "end": 644.82, "word": " في", "probability": 0.984375}], "temperature": 1.0}, {"id": 25, "seek": 67417, "start": 645.25, "end": 674.17, "text": "البط فيه اندهان alkaline و فيه اندهان acidic اللي هو ال yellow في ال triple sugar iron agar ممنوع اشوف هاي ممنوع اشوف ال alkaline جنب ال H2S production لكن في ال lysine iron agar قولنا في حال كان عندي H2S positive ماقدر احدد هل هي alkaline او هل هي acidic اللي هو yellow هل هي عملت اللي هو lysine decarboxylation او عملت gelicose fermentation تمام", "tokens": [6027, 3555, 9566, 8978, 3224, 16472, 3215, 3224, 7649, 44220, 533, 4032, 8978, 3224, 16472, 3215, 3224, 7649, 39514, 13672, 1829, 31439, 2423, 5566, 8978, 2423, 15508, 5076, 6497, 623, 289, 3714, 27842, 45367, 1975, 8592, 38688, 8032, 47302, 3714, 27842, 45367, 1975, 8592, 38688, 2423, 44220, 533, 10874, 1863, 3555, 2423, 389, 17, 50, 4265, 44381, 8978, 2423, 48670, 533, 6497, 623, 289, 12174, 12610, 8315, 8978, 11331, 6027, 25961, 18871, 16254, 389, 17, 50, 3353, 19446, 28543, 2288, 1975, 24401, 3215, 8032, 1211, 39896, 44220, 533, 1975, 2407, 8032, 1211, 39896, 39514, 13672, 1829, 31439, 5566, 8032, 1211, 39896, 6225, 42213, 2655, 13672, 1829, 31439, 48670, 533, 979, 289, 4995, 5088, 399, 1975, 2407, 6225, 42213, 2655, 4087, 299, 541, 43161, 46811, 10943], 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1.0}, {"id": 27, "seek": 71825, "start": 704.45, "end": 718.25, "text": "كان مهم لإنتاج الـ H2 acid production لكن مع ان البكتيريا كان القدرة انها تعمل Lycine decarboxylation فعملت Lycine اللي هو deamination", "tokens": [41361, 3714, 16095, 5296, 28814, 29399, 26108, 2423, 39184, 389, 17, 8258, 4265, 44381, 20449, 16472, 29739, 4117, 2655, 13546, 25528, 25961, 25062, 3215, 25720, 16472, 11296, 6055, 25957, 1211, 12687, 66, 533, 979, 289, 4995, 5088, 399, 6156, 25957, 1211, 2655, 12687, 66, 533, 13672, 1829, 31439, 368, 335, 2486], "avg_logprob": -0.2656250097430669, "compression_ratio": 1.304054054054054, "no_speech_prob": 5.960464477539062e-07, "words": [{"start": 704.45, "end": 704.85, "word": "كان", "probability": 0.9736328125}, {"start": 704.85, "end": 705.27, "word": " مهم", "probability": 0.986328125}, {"start": 705.27, "end": 708.41, "word": " لإنتاج", "probability": 0.76312255859375}, {"start": 708.41, "end": 708.57, "word": " الـ", "probability": 0.86083984375}, {"start": 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"end": 768.82, "text": "بيصير في ظروف بتحفظ في ظروف acidic condition فأي بكتيريا عاملة lysine decarboxylation بالاصل هي بتكون عاملة جليكوز fermentation اتكون acidic condition لازم لاقى الـ H2O acid production و لازم لاقى ال lysine decarboxylation هلأ ال lysine decarboxylation تمام بتكون في عندي مركب قاعدي اللي هو ال cadaverin هذا هيرفعلي ال ال BH وهيحول اللون الوسط للون بارفل", "tokens": [3555, 1829, 9381, 13546, 8978, 1357, 116, 32887, 5172, 39894, 5016, 5172, 19913, 8978, 1357, 116, 32887, 5172, 8258, 299, 4188, 6156, 10721, 1829, 4724, 4117, 2655, 13546, 25528, 6225, 10943, 37977, 48670, 533, 979, 289, 4995, 5088, 399, 20666, 33546, 1211, 39896, 39894, 30544, 6225, 10943, 37977, 10874, 20292, 4117, 2407, 11622, 43161, 1975, 2655, 30544, 8258, 299, 4188, 5296, 31377, 2304, 20193, 4587, 7578, 2423, 39184, 389, 17, 46, 8258, 4265, 4032, 5296, 31377, 2304, 20193, 4587, 7578, 2423, 48670, 533, 979, 289, 4995, 5088, 399, 8032, 1211, 10721, 2423, 48670, 533, 979, 289, 4995, 5088, 399, 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90324, "start": 874.26, "end": 903.24, "text": "Yellow في البط يبقى Gelicose fermentation positive Lycine decarboxylation negative بالنسبة للإسلاند ماعندي لون purple يبقى ما اتكون عندي اللي هو الجيلاتين اللي بالنسبة للإسلاند اللون ال red معناه اللي هو Lycine deamination positive لكن ماعملت جيلاتين deamination يبقى هان اللون ال red في الإسلاند معناه Lycine deamination positive يبقى Yellow A", "tokens": [56, 21348, 8978, 29739, 9566, 7251, 3555, 4587, 7578, 16142, 299, 541, 43161, 3353, 12687, 66, 533, 979, 289, 4995, 5088, 399, 3671, 20666, 1863, 35457, 3660, 24976, 28814, 3794, 1211, 7649, 3215, 19446, 3615, 1863, 16254, 5296, 11536, 9656, 7251, 3555, 4587, 7578, 19446, 1975, 2655, 30544, 18871, 16254, 13672, 1829, 31439, 25724, 26895, 9307, 9957, 13672, 1829, 20666, 1863, 35457, 3660, 24976, 28814, 3794, 1211, 7649, 3215, 13672, 11536, 2423, 2182, 20449, 8315, 3224, 13672, 1829, 31439, 12687, 66, 533, 368, 335, 2486, 3353, 44381, 19446, 25957, 1211, 2655, 10874, 26895, 9307, 9957, 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"probability": 0.82177734375}, {"start": 899.2, "end": 899.6, "word": " الإسلاند", "probability": 0.9654296875}, {"start": 899.6, "end": 900.0, "word": " معناه", "probability": 0.9361979166666666}, {"start": 900.0, "end": 900.56, "word": " Lycine", "probability": 0.9142252604166666}, {"start": 900.56, "end": 901.12, "word": " deamination", "probability": 0.95166015625}, {"start": 901.12, "end": 901.64, "word": " positive", "probability": 0.923828125}, {"start": 901.64, "end": 902.42, "word": " يبقى", "probability": 0.9716796875}, {"start": 902.42, "end": 902.88, "word": " Yellow", "probability": 0.7109375}, {"start": 902.88, "end": 903.24, "word": " A", "probability": 0.378173828125}], "temperature": 1.0}, {"id": 35, "seek": 93039, "start": 903.49, "end": 930.39, "text": "الـ Red قلنا بما أن الـ Red بقى برمز لإله بالرمز R مش الرمز K هاي البكتيريا فقط عندها القدرة أنها تعمل Deamination للليسينومينة عندها القدرة أنها تعمل Decarboxylation للليسينومينة الـ Black precipitate قلنا الـ H2S production هاي ما بقدر أشوفها أو ما بقدر إذا كانت H2S production أحدد هل هي A أو هل هي K هل هي Gelicose fermentation أو هل هي", "tokens": [6027, 39184, 4477, 12174, 1211, 8315, 4724, 15042, 14739, 2423, 39184, 4477, 4724, 4587, 7578, 4724, 2288, 2304, 11622, 5296, 28814, 43761, 20666, 2288, 2304, 11622, 497, 37893, 34892, 2304, 11622, 591, 8032, 47302, 29739, 4117, 2655, 13546, 25528, 6156, 47432, 43242, 11296, 25062, 3215, 25720, 14739, 11296, 6055, 25957, 1211, 1346, 335, 2486, 24976, 20292, 3794, 9957, 20498, 9957, 3660, 43242, 11296, 25062, 3215, 25720, 14739, 11296, 6055, 25957, 1211, 1346, 6166, 4995, 5088, 399, 24976, 20292, 3794, 9957, 20498, 9957, 3660, 2423, 39184, 4076, 23354, 8086, 12174, 1211, 8315, 2423, 39184, 389, 17, 50, 4265, 8032, 47302, 19446, 4724, 28543, 2288, 5551, 8592, 38688, 11296, 34051, 19446, 4724, 28543, 2288, 11933, 15730, 25961, 2655, 389, 17, 50, 4265, 5551, 24401, 3215, 8032, 1211, 39896, 316, 34051, 8032, 1211, 39896, 591, 8032, 1211, 39896, 16142, 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923.99, "end": 924.57, "word": " H2S", "probability": 0.94189453125}, {"start": 924.57, "end": 925.11, "word": " production", "probability": 0.92626953125}, {"start": 925.11, "end": 926.07, "word": " أحدد", "probability": 0.9606119791666666}, {"start": 926.07, "end": 926.31, "word": " هل", "probability": 0.98193359375}, {"start": 926.31, "end": 926.59, "word": " هي", "probability": 0.9384765625}, {"start": 926.59, "end": 926.99, "word": " A", "probability": 0.904296875}, {"start": 926.99, "end": 927.55, "word": " أو", "probability": 0.9033203125}, {"start": 927.55, "end": 927.81, "word": " هل", "probability": 0.936767578125}, {"start": 927.81, "end": 928.01, "word": " هي", "probability": 0.96337890625}, {"start": 928.01, "end": 928.23, "word": " K", "probability": 0.93115234375}, {"start": 928.23, "end": 928.47, "word": " هل", "probability": 0.883544921875}, {"start": 928.47, "end": 928.63, "word": " هي", "probability": 0.95654296875}, {"start": 928.63, "end": 929.07, "word": " 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في ال slant معناه lycindy amination positive اللون ال berbol في البط معناه lycindy carboxylation positive وإحنا قلنا ممنوع البكتيريا تعمل الأثنان مع بعض يبقى اللون ال red في ال slant و ال berbol بالبط ممنوع أني أنا أشوفه", "tokens": [41185, 2423, 39184, 1061, 394, 16070, 1211, 11536, 2423, 5948, 17460, 8978, 29739, 9566, 5296, 33456, 13672, 11536, 2423, 2182, 8978, 2423, 1061, 394, 20449, 8315, 3224, 17293, 66, 471, 88, 669, 2486, 3353, 13672, 11536, 2423, 5948, 17460, 8978, 29739, 9566, 20449, 8315, 3224, 17293, 66, 471, 88, 1032, 4995, 5088, 399, 3353, 4032, 28814, 5016, 8315, 12174, 1211, 8315, 3714, 27842, 45367, 29739, 4117, 2655, 13546, 25528, 6055, 25957, 1211, 16247, 12984, 1863, 7649, 20449, 45030, 11242, 7251, 3555, 4587, 7578, 13672, 11536, 2423, 2182, 8978, 2423, 1061, 394, 4032, 2423, 5948, 17460, 20666, 3555, 9566, 3714, 27842, 45367, 14739, 1829, 41850, 5551, 8592, 38688, 3224], "avg_logprob": -0.1903669697429062, "compression_ratio": 2.0552763819095476, "no_speech_prob": 4.208087921142578e-05, "words": [{"start": 960.05, "end": 960.27, "word": "في", "probability": 0.39892578125}, {"start": 960.27, "end": 960.37, "word": " الـ", "probability": 0.666748046875}, {"start": 960.37, "end": 960.79, "word": " slant", "probability": 0.77099609375}, {"start": 960.79, "end": 961.41, "word": " واللون", "probability": 0.86962890625}, {"start": 961.41, "end": 961.71, "word": " ال", "probability": 0.9794921875}, {"start": 961.71, "end": 962.23, "word": " berbol", "probability": 0.366607666015625}, {"start": 962.23, "end": 962.53, "word": " في", "probability": 0.96484375}, {"start": 962.53, "end": 963.19, "word": " البط", "probability": 0.77783203125}, {"start": 963.19, "end": 964.21, "word": " لأن", "probability": 0.8798828125}, {"start": 964.21, "end": 965.35, "word": " اللون", "probability": 0.940185546875}, {"start": 965.35, "end": 965.47, "word": " ال", "probability": 0.962890625}, {"start": 965.47, "end": 965.59, "word": " red", "probability": 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{"start": 970.83, "end": 971.33, "word": " معناه", "probability": 0.9654947916666666}, {"start": 971.33, "end": 971.95, "word": " lycindy", "probability": 0.8643798828125}, {"start": 971.95, "end": 972.69, "word": " carboxylation", "probability": 0.94921875}, {"start": 972.69, "end": 973.05, "word": " positive", "probability": 0.91748046875}, {"start": 973.05, "end": 973.33, "word": " وإحنا", "probability": 0.7254638671875}, {"start": 973.33, "end": 973.61, "word": " قلنا", "probability": 0.91943359375}, {"start": 973.61, "end": 973.95, "word": " ممنوع", "probability": 0.9720052083333334}, {"start": 973.95, "end": 974.43, "word": " البكتيريا", "probability": 0.96181640625}, {"start": 974.43, "end": 974.77, "word": " تعمل", "probability": 0.94921875}, {"start": 974.77, "end": 975.13, "word": " الأثنان", "probability": 0.788818359375}, {"start": 975.13, "end": 975.29, "word": " مع", "probability": 0.9931640625}, {"start": 975.29, "end": 975.59, "word": " بعض", "probability": 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978.91, "word": " أني", "probability": 0.7548828125}, {"start": 978.91, "end": 979.17, "word": " أنا", "probability": 0.640625}, {"start": 979.17, "end": 980.03, "word": " أشوفه", "probability": 0.9696044921875}], "temperature": 1.0}, {"id": 38, "seek": 101018, "start": 982.02, "end": 1010.18, "text": "هذه تفسير النتائج هنا نلاحظ أن اللون purple في الإسلاند و purple في البط يبقى K على K طبعا تفسير النتائج زي ما قلنا بما أن purple و purple purple في الإسلاند يبقى gelatin purple في الإسلاند يبقى gelatin deamination purple في البط يبقى lysine decarboxylation هنا K على A", "tokens": [3224, 24192, 6055, 36178, 13546, 28239, 2655, 16373, 7435, 34105, 8717, 15040, 5016, 19913, 14739, 13672, 11536, 9656, 8978, 33688, 3794, 1211, 7649, 3215, 4032, 9656, 8978, 29739, 9566, 7251, 3555, 4587, 7578, 591, 15844, 591, 23032, 3555, 3615, 995, 6055, 36178, 13546, 28239, 2655, 16373, 7435, 30767, 1829, 19446, 12174, 1211, 8315, 4724, 15042, 14739, 9656, 4032, 9656, 9656, 8978, 33688, 3794, 1211, 7649, 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"probability": 0.6689453125}, {"start": 987.38, "end": 987.6, "word": " في", "probability": 0.90966796875}, {"start": 987.6, "end": 988.22, "word": " الإسلاند", "probability": 0.734912109375}, {"start": 988.22, "end": 988.36, "word": " و", "probability": 0.82568359375}, {"start": 988.36, "end": 988.66, "word": " purple", "probability": 0.92822265625}, {"start": 988.66, "end": 989.08, "word": " في", "probability": 0.97216796875}, {"start": 989.08, "end": 989.54, "word": " البط", "probability": 0.8818359375}, {"start": 989.54, "end": 990.28, "word": " يبقى", "probability": 0.9000244140625}, {"start": 990.28, "end": 990.6, "word": " K", "probability": 0.5576171875}, {"start": 990.6, "end": 990.86, "word": " على", "probability": 0.6904296875}, {"start": 990.86, "end": 991.22, "word": " K", "probability": 0.962890625}, {"start": 991.22, "end": 991.88, "word": " طبعا", "probability": 0.959228515625}, {"start": 991.88, "end": 992.32, "word": " تفسير", "probability": 0.9822591145833334}, {"start": 992.32, "end": 992.92, "word": " النتائج", "probability": 0.9879150390625}, {"start": 992.92, "end": 993.18, "word": " زي", "probability": 0.841796875}, {"start": 993.18, "end": 993.28, "word": " ما", "probability": 0.99169921875}, {"start": 993.28, "end": 993.66, "word": " قلنا", "probability": 0.9423828125}, {"start": 993.66, "end": 993.98, "word": " بما", "probability": 0.9716796875}, {"start": 993.98, "end": 994.18, "word": " أن", "probability": 0.7578125}, {"start": 994.18, "end": 994.58, "word": " purple", "probability": 0.9384765625}, {"start": 994.58, "end": 994.76, "word": " و", "probability": 0.75732421875}, {"start": 994.76, "end": 995.2, "word": " purple", "probability": 0.94970703125}, {"start": 995.2, "end": 996.28, "word": " purple", "probability": 0.744140625}, {"start": 996.28, "end": 996.6, "word": " في", "probability": 0.97802734375}, {"start": 996.6, "end": 997.24, "word": " الإسلاند", "probability": 0.93115234375}, {"start": 997.24, "end": 997.5, "word": " يبقى", "probability": 0.97265625}, {"start": 997.5, "end": 997.94, "word": " gelatin", "probability": 0.8046875}, {"start": 997.94, "end": 998.98, "word": " purple", "probability": 0.50732421875}, {"start": 998.98, "end": 999.28, "word": " في", "probability": 0.98291015625}, {"start": 999.28, "end": 999.72, "word": " الإسلاند", "probability": 0.95546875}, {"start": 999.72, "end": 999.9, "word": " يبقى", "probability": 0.9864501953125}, {"start": 999.9, "end": 1000.3, "word": " gelatin", "probability": 0.9384765625}, {"start": 1000.3, "end": 1001.18, "word": " deamination", "probability": 0.7591959635416666}, {"start": 1001.18, "end": 1002.16, "word": " purple", "probability": 0.96630859375}, {"start": 1002.16, "end": 1002.56, "word": " في", "probability": 0.970703125}, {"start": 1002.56, "end": 1005.9, "word": " البط", "probability": 0.639892578125}, {"start": 1005.9, "end": 1006.36, "word": " يبقى", "probability": 0.949462890625}, {"start": 1006.36, "end": 1007.08, "word": " lysine", "probability": 0.751953125}, {"start": 1007.08, "end": 1008.66, "word": " decarboxylation", "probability": 0.958203125}, {"start": 1008.66, "end": 1009.28, "word": " هنا", "probability": 0.521484375}, {"start": 1009.28, "end": 1009.54, "word": " K", "probability": 0.8193359375}, {"start": 1009.54, "end": 1009.86, "word": " على", "probability": 0.91162109375}, {"start": 1009.86, "end": 1010.18, "word": " A", "probability": 0.9013671875}], "temperature": 1.0}, {"id": 39, "seek": 103929, "start": 1010.57, "end": 1039.29, "text": "منلاحظ أنه هنا بما أنه في الـBot A يبقى Gelicose fermentation في الـslant هنا K يبقى هي في الـslant تتحول للون للون Violet يبقى هي Gelatin Deamination يبقى هنا البكتيريا ماعندها القدرة أن تتعمل للليسين ولا Deamination ولا Decarboxylation هنا الرمز في عندي Yellow في الـBot يبقى Gelicose fermentation positive ليسين Decarboxylation negative في عندى تشققات ممكن نضيف Gas", "tokens": [27842, 15040, 5016, 19913, 14739, 3224, 34105, 4724, 15042, 14739, 3224, 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اندول ارتبط مع الكوفكريازيران تتكون اندي red ring طب لو كان على سطح لون فيه purple معناه ان البكتيريا عملت deamination لارنيثين اتكون اندي alkaline product حول ال pH indicator لللون ال purple هذا بالنسبة لهاي ال media", "tokens": [6027, 3555, 4117, 2655, 13546, 25528, 43242, 11296, 14739, 11622, 32640, 5296, 2655, 16572, 3555, 2655, 38688, 7649, 31377, 10874, 3794, 43500, 5296, 2655, 16572, 3555, 2655, 38688, 7649, 1975, 2655, 30544, 16472, 3215, 12610, 1975, 43500, 3555, 9566, 20449, 33251, 38688, 4117, 16572, 31377, 13546, 7649, 6055, 2655, 30544, 16472, 16254, 2182, 4875, 23032, 3555, 45164, 25961, 15844, 8608, 9566, 5016, 5296, 11536, 8978, 3224, 9656, 20449, 8315, 3224, 16472, 29739, 4117, 2655, 13546, 25528, 6225, 42213, 2655, 368, 335, 2486, 5296, 9640, 22653, 12984, 9957, 1975, 2655, 30544, 16472, 16254, 44220, 533, 1674, 11331, 12610, 2423, 21677, 16961, 24976, 1211, 11536, 2423, 9656, 23758, 20666, 1863, 35457, 3660, 5296, 11296, 1829, 2423, 3021], "avg_logprob": 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"end": 1239.18, "word": " الكوفكريازيران", "probability": 0.6356026785714286}, {"start": 1239.18, "end": 1239.56, "word": " تتكون", "probability": 0.87109375}, {"start": 1239.56, "end": 1239.96, "word": " اندي", "probability": 0.738525390625}, {"start": 1239.96, "end": 1240.3, "word": " red", "probability": 0.210693359375}, {"start": 1240.3, "end": 1240.78, "word": " ring", "probability": 0.966796875}, {"start": 1240.78, "end": 1241.78, "word": " طب", "probability": 0.8935546875}, {"start": 1241.78, "end": 1242.06, "word": " لو", "probability": 0.96484375}, {"start": 1242.06, "end": 1242.3, "word": " كان", "probability": 0.99609375}, {"start": 1242.3, "end": 1242.5, "word": " على", "probability": 0.869140625}, {"start": 1242.5, "end": 1242.84, "word": " سطح", "probability": 0.84619140625}, {"start": 1242.84, "end": 1243.18, "word": " لون", "probability": 0.7490234375}, {"start": 1243.18, "end": 1243.48, "word": " فيه", "probability": 0.6375732421875}, {"start": 1243.48, "end": 1243.78, "word": " purple", "probability": 0.5341796875}, {"start": 1243.78, "end": 1244.22, "word": " معناه", "probability": 0.9729817708333334}, {"start": 1244.22, "end": 1244.32, "word": " ان", "probability": 0.72119140625}, {"start": 1244.32, "end": 1244.8, "word": " البكتيريا", "probability": 0.9763671875}, {"start": 1244.8, "end": 1245.12, "word": " عملت", "probability": 0.9664713541666666}, {"start": 1245.12, "end": 1245.82, "word": " deamination", "probability": 0.7083333333333334}, {"start": 1245.82, "end": 1247.0, "word": " لارنيثين", "probability": 0.67177734375}, {"start": 1247.0, "end": 1247.38, "word": " اتكون", "probability": 0.896484375}, {"start": 1247.38, "end": 1247.68, "word": " اندي", "probability": 0.8486328125}, {"start": 1247.68, "end": 1248.22, "word": " alkaline", "probability": 0.54327392578125}, {"start": 1248.22, "end": 1248.7, "word": " product", "probability": 0.92236328125}, {"start": 1248.7, "end": 1249.7, "word": " حول", "probability": 0.877685546875}, {"start": 1249.7, "end": 1249.92, "word": " ال", "probability": 0.9697265625}, {"start": 1249.92, "end": 1250.24, "word": " pH", "probability": 0.54248046875}, {"start": 1250.24, "end": 1250.72, "word": " indicator", "probability": 0.8466796875}, {"start": 1250.72, "end": 1251.38, "word": " لللون", "probability": 0.8468424479166666}, {"start": 1251.38, "end": 1251.64, "word": " ال", "probability": 0.8994140625}, {"start": 1251.64, "end": 1251.92, "word": " purple", "probability": 0.93896484375}, {"start": 1251.92, "end": 1252.26, "word": " هذا", "probability": 0.78857421875}, {"start": 1252.26, "end": 1252.84, "word": " بالنسبة", "probability": 0.988037109375}, {"start": 1252.84, "end": 1254.14, "word": " لهاي", "probability": 0.63330078125}, {"start": 1254.14, "end": 1254.32, "word": " ال", "probability": 0.763671875}, {"start": 1254.32, "end": 1254.62, "word": " media", "probability": 0.91162109375}], "temperature": 1.0}, {"id": 48, "seek": 128371, "start": 1255.09, "end": 1283.71, "text": "نشوف مكونات ال media أكيد yes extract كمصادر للتغذية بيبتون تمام لأن البكتيري هتعمل ال ال deamination للبيبتون sorry في هذه الفقرة نمسح ال ornithine deamination و نكتب بيبتون deamination هان بيبتون deamination مش decarboxylation", "tokens": [1863, 8592, 38688, 3714, 4117, 2407, 8315, 2655, 2423, 3021, 5551, 4117, 25708, 2086, 8947, 9122, 2304, 9381, 18513, 2288, 24976, 2655, 17082, 8848, 10632, 4724, 1829, 3555, 2655, 11536, 46811, 10943, 5296, 33456, 29739, 4117, 2655, 13546, 1829, 8032, 2655, 25957, 1211, 2423, 2423, 368, 335, 2486, 24976, 21292, 3555, 2655, 11536, 2597, 8978, 29538, 27188, 4587, 25720, 8717, 2304, 3794, 5016, 2423, 420, 77, 355, 533, 368, 335, 2486, 4032, 8717, 4117, 2655, 3555, 4724, 1829, 3555, 2655, 11536, 368, 335, 2486, 8032, 7649, 4724, 1829, 3555, 2655, 11536, 368, 335, 2486, 37893, 979, 289, 4995, 5088, 399], "avg_logprob": -0.249690584617086, "compression_ratio": 1.6954314720812182, "no_speech_prob": 1.7881393432617188e-07, "words": [{"start": 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1261.91, "end": 1263.23, "word": " البكتيري", "probability": 0.723291015625}, {"start": 1263.23, "end": 1263.87, "word": " هتعمل", "probability": 0.897216796875}, {"start": 1263.87, "end": 1264.09, "word": " ال", "probability": 0.68896484375}, {"start": 1264.09, "end": 1265.13, "word": " ال", "probability": 0.355712890625}, {"start": 1265.13, "end": 1266.09, "word": " deamination", "probability": 0.6835123697916666}, {"start": 1266.09, "end": 1266.91, "word": " للبيبتون", "probability": 0.891015625}, {"start": 1266.91, "end": 1267.65, "word": " sorry", "probability": 0.25927734375}, {"start": 1267.65, "end": 1267.89, "word": " في", "probability": 0.87109375}, {"start": 1267.89, "end": 1268.31, "word": " هذه", "probability": 0.8125}, {"start": 1268.31, "end": 1269.19, "word": " الفقرة", "probability": 0.9581705729166666}, {"start": 1269.19, "end": 1271.11, "word": " نمسح", "probability": 0.9781494140625}, {"start": 1271.11, "end": 1271.25, "word": " ال", "probability": 0.70849609375}, {"start": 1271.25, "end": 1271.95, "word": " ornithine", "probability": 0.69537353515625}, {"start": 1271.95, "end": 1272.95, "word": " deamination", "probability": 0.7030436197916666}, {"start": 1272.95, "end": 1273.15, "word": " و", "probability": 0.96044921875}, {"start": 1273.15, "end": 1273.67, "word": " نكتب", "probability": 0.8824462890625}, {"start": 1273.67, "end": 1274.15, "word": " بيبتون", "probability": 0.905078125}, {"start": 1274.15, "end": 1276.27, "word": " deamination", "probability": 0.8854166666666666}, {"start": 1276.27, "end": 1280.83, "word": " هان", "probability": 0.34136962890625}, {"start": 1280.83, "end": 1281.61, "word": " بيبتون", "probability": 0.9501953125}, {"start": 1281.61, "end": 1282.33, "word": " deamination", "probability": 0.876953125}, {"start": 1282.33, "end": 1282.59, "word": " مش", "probability": 0.9482421875}, {"start": 1282.59, "end": 1283.71, "word": " decarboxylation", "probability": 0.82900390625}], "temperature": 1.0}, {"id": 49, "seek": 130296, "start": 1286.08, "end": 1302.96, "text": "مش ornithine هان اللي هي ال peptone دي amination و هان اللي هي برضه ال peptone دي amination تمام؟ بدي أكشف في هذه ال media عن قدرة البكتيريا أنها تعمل decarboxylation لل ornithine لو ماعملت decarboxylation هتعمل deamination لل peptone في ال European condition", "tokens": [2304, 8592, 420, 77, 355, 533, 8032, 7649, 13672, 1829, 39896, 2423, 520, 662, 546, 11778, 1829, 669, 2486, 4032, 8032, 7649, 13672, 1829, 39896, 4724, 43042, 3224, 2423, 520, 662, 546, 11778, 1829, 669, 2486, 46811, 10943, 22807, 4724, 16254, 5551, 4117, 8592, 5172, 8978, 29538, 2423, 3021, 18871, 12174, 3215, 25720, 29739, 4117, 2655, 13546, 25528, 14739, 11296, 6055, 25957, 1211, 979, 289, 4995, 5088, 399, 24976, 420, 77, 355, 533, 45164, 19446, 25957, 1211, 2655, 979, 289, 4995, 5088, 399, 8032, 2655, 25957, 1211, 368, 335, 2486, 24976, 520, 662, 546, 8978, 2423, 6473, 4188], "avg_logprob": -0.24131945106718275, "compression_ratio": 1.8298969072164948, "no_speech_prob": 3.635883331298828e-05, "words": [{"start": 1286.08, "end": 1286.44, "word": "مش", "probability": 0.834228515625}, {"start": 1286.44, "end": 1287.08, "word": " ornithine", "probability": 0.60284423828125}, {"start": 1287.08, "end": 1287.42, "word": " هان", "probability": 0.59765625}, {"start": 1287.42, "end": 1287.56, "word": " اللي", "probability": 0.7724609375}, {"start": 1287.56, "end": 1287.8, "word": " هي", "probability": 0.7841796875}, {"start": 1287.8, "end": 1288.02, "word": " ال", "probability": 0.67138671875}, {"start": 1288.02, "end": 1288.56, "word": " peptone", "probability": 0.5672200520833334}, {"start": 1288.56, "end": 1288.78, "word": " دي", "probability": 0.646728515625}, {"start": 1288.78, "end": 1289.28, "word": " amination", "probability": 0.63330078125}, {"start": 1289.28, "end": 1289.5, "word": " و", "probability": 0.55322265625}, {"start": 1289.5, "end": 1289.86, "word": " هان", "probability": 0.779296875}, {"start": 1289.86, "end": 1290.08, "word": " اللي", "probability": 0.973388671875}, {"start": 1290.08, "end": 1290.3, "word": " هي", "probability": 0.76953125}, {"start": 1290.3, "end": 1290.64, "word": " برضه", "probability": 0.8680013020833334}, {"start": 1290.64, "end": 1290.76, "word": " ال", "probability": 0.9404296875}, {"start": 1290.76, "end": 1291.22, "word": " peptone", "probability": 0.8898111979166666}, {"start": 1291.22, "end": 1291.42, "word": " دي", "probability": 0.96630859375}, {"start": 1291.42, "end": 1291.94, "word": " amination", "probability": 0.9365234375}, {"start": 1291.94, "end": 1292.82, "word": " تمام؟", "probability": 0.7674967447916666}, {"start": 1292.82, "end": 1293.08, "word": " بدي", "probability": 0.766357421875}, {"start": 1293.08, "end": 1293.5, "word": " أكشف", "probability": 0.9306640625}, {"start": 1293.5, "end": 1293.74, "word": " في", "probability": 0.96728515625}, {"start": 1293.74, "end": 1293.94, "word": " هذه", "probability": 0.66845703125}, {"start": 1293.94, "end": 1294.06, "word": " ال", "probability": 0.85986328125}, {"start": 1294.06, "end": 1294.28, "word": " media", "probability": 0.9140625}, {"start": 1294.28, "end": 1294.46, "word": " عن", "probability": 0.90771484375}, {"start": 1294.46, "end": 1294.9, "word": " قدرة", "probability": 0.9138997395833334}, {"start": 1294.9, "end": 1295.56, "word": " البكتيريا", "probability": 0.92392578125}, {"start": 1295.56, "end": 1295.76, "word": " أنها", "probability": 0.6624755859375}, {"start": 1295.76, "end": 1296.1, "word": " تعمل", "probability": 0.9752604166666666}, {"start": 1296.1, "end": 1297.08, "word": " decarboxylation", "probability": 0.93369140625}, {"start": 1297.08, "end": 1297.34, "word": " لل", "probability": 0.337646484375}, {"start": 1297.34, "end": 1298.08, "word": " ornithine", "probability": 0.844970703125}, {"start": 1298.08, "end": 1298.22, "word": " لو", "probability": 0.87353515625}, {"start": 1298.22, "end": 1298.68, "word": " ماعملت", "probability": 0.8369140625}, {"start": 1298.68, "end": 1299.52, "word": " decarboxylation", "probability": 0.97294921875}, {"start": 1299.52, "end": 1299.98, "word": " هتعمل", "probability": 0.96337890625}, {"start": 1299.98, "end": 1300.58, "word": " deamination", "probability": 0.7854817708333334}, {"start": 1300.58, "end": 1300.8, "word": " لل", "probability": 0.94677734375}, {"start": 1300.8, "end": 1301.36, "word": " peptone", "probability": 0.7928059895833334}, {"start": 1301.36, "end": 1301.96, "word": " في", "probability": 0.97802734375}, {"start": 1301.96, "end": 1302.12, "word": " ال", "probability": 0.88232421875}, {"start": 1302.12, "end": 1302.42, "word": " European", "probability": 0.163330078125}, {"start": 1302.42, "end": 1302.96, "word": " condition", "probability": 0.857421875}], "temperature": 1.0}, {"id": 50, "seek": 133165, "start": 1306.33, "end": 1331.65, "text": "بالنسبة لأ ال Ornithin موجود أكيد في ال media لأنها أكشف عن قدرة البكتيريا أن تعمل دي Carboxylation ال Dextrose موجود بكمية قليلة عشان أكشف على ال fermentation الأجار بما أن فحص Motility يبقى هي semi-solid media نسبة الأجار 4 من 10% ال pH indicator ال برامو سرزال بيربول قلنا حدوده شو بتكون في ال acidic و ال basic", "tokens": [3555, 6027, 1863, 35457, 3660, 5296, 10721, 2423, 1610, 77, 355, 259, 3714, 29245, 23328, 5551, 4117, 25708, 8978, 2423, 3021, 5296, 33456, 11296, 5551, 4117, 8592, 5172, 18871, 12174, 3215, 25720, 29739, 4117, 2655, 13546, 25528, 14739, 6055, 25957, 1211, 11778, 1829, 2741, 4995, 5088, 399, 2423, 1346, 734, 37841, 3714, 29245, 23328, 4724, 24793, 10632, 12174, 20292, 37977, 6225, 8592, 7649, 5551, 4117, 8592, 5172, 15844, 2423, 43161, 16247, 7435, 9640, 4724, 15042, 14739, 6156, 5016, 9381, 8956, 1140, 7251, 3555, 4587, 7578, 39896, 12909, 12, 30926, 327, 3021, 8717, 35457, 3660, 16247, 7435, 9640, 1017, 9154, 1266, 4, 2423, 21677, 16961, 2423, 4724, 2288, 10943, 2407, 8608, 2288, 11622, 6027, 4724, 13546, 3555, 12610, 12174, 1211, 8315, 11331, 3215, 23328, 3224, 13412, 2407, 39894, 30544, 8978, 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0.388916015625}, {"start": 1825.97, "end": 1826.55, "word": " Single", "probability": 0.63330078125}, {"start": 1826.55, "end": 1827.09, "word": " Media", "probability": 0.84130859375}, {"start": 1827.09, "end": 1827.51, "word": " Multiple", "probability": 0.89453125}, {"start": 1827.51, "end": 1828.09, "word": " Test", "probability": 0.8447265625}, {"start": 1828.09, "end": 1829.19, "word": " يعطيكم", "probability": 0.818115234375}, {"start": 1829.19, "end": 1829.73, "word": " العافية", "probability": 0.95703125}, {"start": 1829.73, "end": 1830.17, "word": " والسلام", "probability": 0.9508463541666666}, {"start": 1830.17, "end": 1830.59, "word": " عليكم", "probability": 0.935546875}, {"start": 1830.59, "end": 1830.97, "word": " ورحمة", "probability": 0.974853515625}, {"start": 1830.97, "end": 1831.23, "word": " الله", "probability": 0.96923828125}, {"start": 1831.23, "end": 1831.99, "word": " وبركاته", "probability": 0.98154296875}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 1833.14575, "duration_after_vad": 1796.4956249999964}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/_HOd9t_eBH4_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/_HOd9t_eBH4_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..b0d8cb2d8205ec054af18570536908e70146425e
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/_HOd9t_eBH4_raw.srt
@@ -0,0 +1,1816 @@
+1
+00:00:01,890 --> 00:00:04,990
+من الأمثلة الأخرى على single media multiple test
+
+2
+00:00:04,990 --> 00:00:10,690
+ليسين آيرون أجار ليسين آيرون أجار من اسمها أكيد في
+
+3
+00:00:10,690 --> 00:00:14,690
+الميديا في عندي ليسين الليسين يعتبر amino acid في
+
+4
+00:00:14,690 --> 00:00:18,290
+الميديا هى بدي أكشف هل البكتيريا بتعمل deamination
+
+5
+00:00:18,290 --> 00:00:23,190
+للليسين او decarboxylation للليسين آيرون بما أنه
+
+6
+00:00:23,190 --> 00:00:26,830
+في آيرون في اسم الميديا يبقى في عندي H2S
+
+7
+00:00:26,830 --> 00:00:31,720
+production بدي أكشف على الـH2S أجار يبقىالـ
+
+8
+00:00:31,720 --> 00:00:35,440
+Solidifying agent الموجود في الميديا هو الأجار
+
+9
+00:00:35,440 --> 00:00:42,120
+بنسبة 1.5% فبالتالي هصب الميديا في Slant YouTube
+
+10
+00:00:42,120 --> 00:00:46,560
+عشان أوفر European condition من خلال Slant لأنه
+
+11
+00:00:46,560 --> 00:00:49,120
+بدي أكشف في هذه الميديا على الـ deamination
+
+12
+00:00:49,120 --> 00:00:53,800
+للليسين والـ deamination للجيلاتين وهدول بيسيروا
+
+13
+00:00:53,800 --> 00:00:59,090
+في الـ European condition كمان بدي أنا أكشف علىالـ
+
+14
+00:00:59,090 --> 00:01:02,130
+Decarboxylation للليسين والـ Fermentation للجليكوز
+
+15
+00:01:02,130 --> 00:01:05,350
+وهدول بيصيروا في الآن Europe Condition اللي من
+
+16
+00:01:05,350 --> 00:01:10,030
+خلال البط بيوفر هاي الظروف في هاي الميديا في
+
+17
+00:01:10,030 --> 00:01:12,830
+Indicator بما أنه بكشف على ال fermentation
+
+18
+00:01:12,830 --> 00:01:17,730
+للجليكوز فأكيد لازم وكمان هكشف على ال Deamination
+
+19
+00:01:17,730 --> 00:01:21,310
+للليسين أو الجيلاتين فبالتالي هيتكون acidic or
+
+20
+00:01:21,310 --> 00:01:25,920
+alkaline product فلازم يكون في عندي BH Indicatorال
+
+21
+00:01:25,920 --> 00:01:29,500
+بي اتش انديكاتور الموجود في هاي الميديا اول مرة
+
+22
+00:01:29,500 --> 00:01:34,320
+يمر معنا برامو سارزال بيربل هذا ال بي اتش
+
+23
+00:01:34,320 --> 00:01:38,260
+انديكاتور في ال acidic condition بيكون لونه يلو في
+
+24
+00:01:38,260 --> 00:01:41,320
+ال basic condition بيكون لونه بيربل في ال neutral
+
+25
+00:01:41,320 --> 00:01:46,670
+بيكون لونه اجري او بيكون لونه فيولت فاتحقلنا في
+
+26
+00:01:46,670 --> 00:01:50,750
+عندي file media هكشف على ال H2S production فأكيد
+
+27
+00:01:50,750 --> 00:01:55,390
+لازم يكون H2S indicator اللي هو الferric ammonium
+
+28
+00:01:55,390 --> 00:02:00,710
+citrate تمام هذا بالنسبة لمكونات ال media وعن شوه
+
+29
+00:02:00,710 --> 00:02:05,310
+بتكشف ال media أول أشياء البكتيريا هتلجأ لإله
+
+30
+00:02:05,310 --> 00:02:10,990
+كمصدر للطاقة و للغضاء اللي هو الجليكوز فلو
+
+31
+00:02:10,990 --> 00:02:13,370
+البكتيريا عندها القدرة انها تعمل fermentation
+
+32
+00:02:13,370 --> 00:02:19,170
+للجليكوزهيتكون عندى product acidic هيتحول لون ال
+
+33
+00:02:19,170 --> 00:02:23,770
+media للون yellow يبقى في حال كان في عندي جليكوز
+
+34
+00:02:23,770 --> 00:02:28,770
+fermentation positive يبقى هيتحول لون البط بما انه
+
+35
+00:02:28,770 --> 00:02:33,270
+صار في ظروف لاهوائية هيتحول لون البط للون yellow
+
+36
+00:02:33,270 --> 00:02:37,290
+تانى إشي خلص الجليكوز الجليكوز موجود في ال media
+
+37
+00:02:37,290 --> 00:02:41,110
+بكمية قليلة نفس ل triple sugar iron agar موجود
+
+38
+00:02:41,110 --> 00:02:46,770
+بالنسبة 1 من 10% فالبكتيريافي حال انه خلص
+
+39
+00:02:46,770 --> 00:02:51,030
+الجليكواز اكيد هتلجأ لمصادر اخرى للحصول على الطاقة
+
+40
+00:02:51,030 --> 00:02:55,970
+و للتغذية فبالتالي هتروح للايسين اللايسين موجود في
+
+41
+00:02:55,970 --> 00:02:58,610
+ال media وهي ال media انا بدي اكشف عن قدرة
+
+42
+00:02:58,610 --> 00:03:01,610
+البكتيريا انها تعمل deamination للايسين او
+
+43
+00:03:01,610 --> 00:03:06,370
+decarboxylation او اذا لاحظين انه مكتوب او ممنوع
+
+44
+00:03:06,370 --> 00:03:10,710
+ان البكتيريا يكون عندها القدرة انها تعمل للايسين
+
+45
+00:03:10,710 --> 00:03:14,400
+deamination و decarboxylation مع بعضإما هتعمل de
+
+46
+00:03:14,400 --> 00:03:17,900
+-amination أو de-carboxylation أو ممكن ماتعمل أي
+
+47
+00:03:17,900 --> 00:03:20,840
+إشي لأ اللايسين لكن الأثنين مع بعض ممنوع أني أنا
+
+48
+00:03:20,840 --> 00:03:25,360
+أشوفهم هلأ ال de-amination لالليسين هيتكون عندي
+
+49
+00:03:25,360 --> 00:03:30,340
+alkaline product تمام المفترض بما أنه اتكون عندي
+
+50
+00:03:30,340 --> 00:03:34,540
+alkaline product هيرفع ال pH هيتحول لون ال media
+
+51
+00:03:34,540 --> 00:03:38,020
+للون purple لكن إذا من اللحظة إذا بتلاحظوا مكتوب
+
+52
+00:03:38,020 --> 00:03:42,480
+هنا بتحول لون ال media للون redطيب ليش اتحول لون
+
+53
+00:03:42,480 --> 00:03:45,460
+ال media للون Red بالرغم انه اتكون عندى alkaline
+
+54
+00:03:45,460 --> 00:03:50,560
+product وفيه عندى BH Indicator موجود في ال acid في
+
+55
+00:03:50,560 --> 00:03:53,040
+sorry في ال basic condition بيكون لونه purple
+
+56
+00:03:53,040 --> 00:03:57,140
+بقوللي ال alkaline product الناتج من عملية ال
+
+57
+00:03:57,140 --> 00:04:02,560
+lysine deamination قوة ارتباطه بال H2S Indicator
+
+58
+00:04:02,560 --> 00:04:06,140
+أعلى من قوة ارتباطه بال BH Indicator فبالتالي
+
+59
+00:04:06,140 --> 00:04:10,130
+هيتكون اللون ال Redنتيجة أن الـAlkaline Product
+
+60
+00:04:10,130 --> 00:04:16,530
+لـLysine Deamination قوة ارتباطه بالـH2S Indicator
+
+61
+00:04:16,530 --> 00:04:21,690
+أعلى من الـBH Indicator فهيتحول اللون للون Red طب
+
+62
+00:04:21,690 --> 00:04:23,830
+لو البكتيريا عندها القدرة انها تعمل
+
+63
+00:04:23,830 --> 00:04:26,610
+Decarboxylation للليسين وما عندها القدرة انها تعمل
+
+64
+00:04:26,610 --> 00:04:31,130
+Deamination للليسين نتيجة عملية الـDecarboxylation
+
+65
+00:04:31,130 --> 00:04:35,210
+برضه هيتثور ان عندي Alkaline Productموجود في عندي
+
+66
+00:04:35,210 --> 00:04:38,150
+pH Indicator في ال basic condition بيكون لونه
+
+67
+00:04:38,150 --> 00:04:42,950
+purple فهي تحول لون ال media للون purple طيب لو
+
+68
+00:04:42,950 --> 00:04:46,110
+البكتيريا ماعندها القدرة انها تعمل لليسين اي اشي
+
+69
+00:04:46,110 --> 00:04:49,370
+سواء deamination او decarboxylation بقوللي هيروح
+
+70
+00:04:49,370 --> 00:04:52,810
+هتروح البكتيريا لأ الجيلاتين الموجود في ال media
+
+71
+00:04:52,810 --> 00:04:56,430
+يعتبر protein هتعمله deamination هيتكون عندي
+
+72
+00:04:56,430 --> 00:05:00,780
+alkaline productموجود عندي pH Indicator في ال
+
+73
+00:05:00,780 --> 00:05:06,140
+basic condition اللي هو لونه purple فبالتالي هيرفع
+
+74
+00:05:06,140 --> 00:05:09,360
+ال pH هذا ال alkaline product هيتحول لون ال media
+
+75
+00:05:09,360 --> 00:05:15,500
+للون purple طيب نيجي للنتائج هلأ ال media فيه عندي
+
+76
+00:05:15,500 --> 00:05:18,980
+slant وbutt قولنا ال slant بتوفر ظروف aerobic
+
+77
+00:05:18,980 --> 00:05:22,080
+condition فالbutt بتوفر ظروف uneurobic condition
+
+78
+00:05:22,610 --> 00:05:26,730
+هلأ بمسك اليوتيوب بتطلع على ال slant لو اتكوّن لون
+
+79
+00:05:26,730 --> 00:05:31,070
+red يبقى بحكي انه صار في عندي Lycine deamination
+
+80
+00:05:31,070 --> 00:05:35,550
+برمز إيه بالرمز A لو اتكوّن لون purple بحكي صار في
+
+81
+00:05:35,550 --> 00:05:40,690
+عندي جيلاتين deamination برمز لإيه بالرمز K لأنه
+
+82
+00:05:40,690 --> 00:05:43,370
+إحنا قلنا اللي Lycine deamination والجيلاتين
+
+83
+00:05:43,370 --> 00:05:48,140
+deamination بيصيروا في ظروف aerobic conditionوقلنا
+
+84
+00:05:48,140 --> 00:05:51,760
+ناتج عملية الـ glycine deamination اللي هو بـ
+
+85
+00:05:51,760 --> 00:05:55,660
+alkaline ال product قوة ارتباطه بالـ H2S Indicator
+
+86
+00:05:55,660 --> 00:05:58,340
+أعلى من الـ BH Indicator فبالتالي هيتحول اللون
+
+87
+00:05:58,340 --> 00:06:01,320
+للون Red الجيلاتين دي amination قولنا بتكون
+
+88
+00:06:01,320 --> 00:06:04,520
+alkaline ال product بيرفع ال BH فبتتحول لون ال
+
+89
+00:06:04,520 --> 00:06:08,520
+media للون بارد طيب بالنسبة للبط قولنا بتوفر Un
+
+90
+00:06:08,520 --> 00:06:10,580
+-Irritable Condition بتكشف على الجليكوز
+
+91
+00:06:10,580 --> 00:06:13,200
+fermentation والـ glycine decarboxylation إذا
+
+92
+00:06:13,200 --> 00:06:17,090
+اتحول لون البط للون yellow يبقى back topبفهم إنه
+
+93
+00:06:17,090 --> 00:06:20,270
+صار في عندي جليكوز fermentation و برمز لإله بالرمز
+
+94
+00:06:20,270 --> 00:06:24,570
+A لو اتحول لون البطل لون purple يبقى بعرف إنه صار
+
+95
+00:06:24,570 --> 00:06:28,150
+في عندي ليسين D-carboxylation و برمز لإله بالرمز K
+
+96
+00:06:28,150 --> 00:06:33,230
+طيب بالنسبة ل ال H2O acid production قولنا ال H2O
+
+97
+00:06:33,230 --> 00:06:35,670
+acid production بيصير في ظروف un-aerobic condition
+
+98
+00:06:35,670 --> 00:06:39,660
+و بيصير في ظروف acidic conditionلو اتكون عندى الـ
+
+99
+00:06:39,660 --> 00:06:43,740
+H2S production مش هعرف هل البكتيريا عملت Gelicose
+
+100
+00:06:43,740 --> 00:06:47,560
+fermentation او Lysine decarboxylation ممكن تكون
+
+101
+00:06:47,560 --> 00:06:50,800
+عملت Gelicose fermentation وممكن تكون عملت Lysine
+
+102
+00:06:50,800 --> 00:06:54,100
+decarboxylation طيب ليش ماقولنا زى ميديا تلثر
+
+103
+00:06:54,100 --> 00:06:57,840
+بالشوبر ايرون اجار من حد ما اشوف الـH2S production
+
+104
+00:06:57,840 --> 00:07:01,340
+بكتب جنبها رمز الـA ممنوع اني انا اشوف رمز الـK
+
+105
+00:07:01,340 --> 00:07:04,880
+بقوللي لأ في هى ال media ممكن يكون A وممكن يكون K
+
+106
+00:07:04,880 --> 00:07:09,820
+ممكن يكون A وممكن يكون Kيبقى الـ H2O production
+
+107
+00:07:09,820 --> 00:07:13,700
+ممكن لأنها تكون عملت Gelicose fermentation رمزها
+
+108
+00:07:13,700 --> 00:07:18,020
+اللى اللى هو البط ممكن يكون ال ICD-carboxylation
+
+109
+00:07:18,020 --> 00:07:23,700
+كام لش هلأ ال ال ال بكتيريا اللى عندها القدرة لو
+
+110
+00:07:23,700 --> 00:07:27,780
+كان الرمز هلأ اه ومعها H2O production معناه ان
+
+111
+00:07:27,780 --> 00:07:31,180
+البكتيريا عملت Gelicose fermentation اتكون عندى
+
+112
+00:07:31,180 --> 00:07:35,080
+acidic condition فبالتالي صار في عندى H2O
+
+113
+00:07:35,080 --> 00:07:38,800
+productionبالنسبة للـ Lysin-D Carboxylation هذا
+
+114
+00:07:38,800 --> 00:07:41,940
+بتكون alkaline product واحنا قلنا الـ H2S
+
+115
+00:07:41,940 --> 00:07:46,400
+production بيحصل في ظروف acidic condition كيف
+
+116
+00:07:46,400 --> 00:07:49,640
+بنحكي أنه عادي ممكن أني أنا أشوف الـ H2S
+
+117
+00:07:49,640 --> 00:07:52,200
+production مع الكائنات، مع الـ Lysin-D
+
+118
+00:07:52,200 --> 00:07:56,320
+Carboxylation؟ بقولي قبل ما البكتيريا تعمل Lysin-D
+
+119
+00:07:56,320 --> 00:07:59,640
+Carboxylation بتكون في الأصل عملت Gelicose
+
+120
+00:07:59,640 --> 00:08:04,800
+fermentationعملت جليكوس fermentation تمام اتكون
+
+121
+00:08:04,800 --> 00:08:07,800
+عندي acidic condition هذا ال acidic condition
+
+122
+00:08:07,800 --> 00:08:12,040
+اتوفر فبالتالي البكتيريا عملت H2O production وعملت
+
+123
+00:08:12,040 --> 00:08:14,660
+lysine decarboxylation لأن ال lysine
+
+124
+00:08:14,660 --> 00:08:18,180
+decarboxylation بيصير برضه نفس ال H2O production
+
+125
+00:08:18,180 --> 00:08:22,920
+في ظروف acidic condition ال lysine decarboxylation
+
+126
+00:08:22,920 --> 00:08:27,880
+نفس ال H2O production بيحفظ في ظروف acidic
+
+127
+00:08:27,880 --> 00:08:32,000
+conditionفبالتالي من حد ما اشوف ان والله البكتيريا
+
+128
+00:08:32,000 --> 00:08:35,100
+صار في عندها او عملة lysin-d-carboxylation ده وهي
+
+129
+00:08:35,100 --> 00:08:37,540
+بالاصل بتكون قبلها عاملة gelicose fermentation
+
+130
+00:08:37,540 --> 00:08:41,900
+اتكون عندى acidic condition اتحفظ ال lysin-d
+
+131
+00:08:41,900 --> 00:08:45,660
+-carboxylation واتحفظ اللى هو ال H2S production
+
+132
+00:08:45,660 --> 00:08:48,340
+اللى لازم يلاقيلهم اتنين acidic condition اللى
+
+133
+00:08:48,340 --> 00:08:51,180
+ناتج من عملية ال gelicose fermentation يبقى في هاي
+
+134
+00:08:51,180 --> 00:08:55,520
+الميديا لو عندى اتكون ال H2Sممكن تكون في الأصل هي
+
+135
+00:08:55,520 --> 00:08:58,680
+Gelicose Fermentation A وممكن تكون رمزها K اللي هي
+
+136
+00:08:58,680 --> 00:09:01,560
+عملت Lycine Decarboxylation وقبل عملت Gelicose
+
+137
+00:09:01,560 --> 00:09:07,100
+Fermentation طيب مكونات ال media yeast extract
+
+138
+00:09:07,100 --> 00:09:10,740
+لازم لنمو البكتيريا Dextrose اللي هو موجود بكمية
+
+139
+00:09:10,740 --> 00:09:15,100
+قليلة 1 من 10% عشان البكتيريا تعمل fermentation ال
+
+140
+00:09:15,100 --> 00:09:17,560
+Lycine اللي هو البكتيريا اللي هيكشف هل هتعمله
+
+141
+00:09:17,560 --> 00:09:21,160
+decarboxylation أو deamination موجود بنسبة 1%
+
+142
+00:09:21,680 --> 00:09:24,180
+Gelatin اللي هو قلنا البروتين اللي هتعمله دي
+
+143
+00:09:24,180 --> 00:09:27,900
+Amination H2S Indicator Ferric Ammonium Citrate
+
+144
+00:09:27,900 --> 00:09:30,720
+السبستريات لهذا الفحص Sodium Thiosulfate الـ BH
+
+145
+00:09:30,720 --> 00:09:35,080
+Indicator البروموس الذي هو البربول الأجاركاس والـ
+
+146
+00:09:35,080 --> 00:09:38,360
+Divying Agent الـ BH للميديا من غير ما أنا أزراحها
+
+147
+00:09:38,360 --> 00:09:42,340
+ستة أو سبعة نلاحظ أن لون الميديا في الأصل من قبل
+
+148
+00:09:42,340 --> 00:09:48,960
+ما أنا أزراحها بيكون لونها بنفسي فاتح هذه الميديا
+
+149
+00:09:48,960 --> 00:09:52,540
+بدي أكشف أنقدرة الـ Gram Negative plus الـ Lie
+
+150
+00:09:52,540 --> 00:09:56,800
+إنها تعمل D-Carboxylate او D-Aminate للـ Lysine هى
+
+151
+00:09:56,800 --> 00:10:00,120
+R مش And يبقى البكتيريا إما هتعمل D-Carboxylation
+
+152
+00:10:00,120 --> 00:10:03,860
+للـ Lysine أو هتعمل D-Amination ممنوع التنان
+
+153
+00:10:03,860 --> 00:10:07,320
+يكونوا مع بعض مش catably and R ممكن هذا أو هذا أو
+
+154
+00:10:07,320 --> 00:10:11,560
+ممكن ماهي تعمل للتنان أي ممكن ماهي تعمل D
+
+155
+00:10:11,560 --> 00:10:14,720
+-Carboxylation ولا D-Amination كمان بدي أكشف عن
+
+156
+00:10:14,720 --> 00:10:18,980
+قدرتها إنها تكون H2S productionبقول إن الليسين دي
+
+157
+00:10:18,980 --> 00:10:21,500
+ammunition بيصير قلنا في ال aerobic condition
+
+158
+00:10:21,500 --> 00:10:25,200
+فبالتالي هاكشف عنه في ال slant ال decarboxylation
+
+159
+00:10:25,200 --> 00:10:28,780
+بيصير في ظروف unaerobic فبالتالي هاكشف عنه في البط
+
+160
+00:10:28,780 --> 00:10:32,140
+السلمونيل أو ال citrobacter إذا ملاحظين التنتين
+
+161
+00:10:32,140 --> 00:10:36,140
+H2S positive يبقى السلمونيل اللي هيمرت معنا ال
+
+162
+00:10:36,140 --> 00:10:40,020
+brute yes H2S positive من الكوليفارم ال
+
+163
+00:10:40,020 --> 00:10:44,540
+citrobacter H2S positive إذا ملاحظ هان جنب ال H2S
+
+164
+00:10:44,540 --> 00:10:48,370
+فيالبط فيه اندهان alkaline و فيه اندهان acidic
+
+165
+00:10:48,370 --> 00:10:51,710
+اللي هو ال yellow في ال triple sugar iron agar
+
+166
+00:10:51,710 --> 00:10:55,470
+ممنوع اشوف هاي ممنوع اشوف ال alkaline جنب ال H2S
+
+167
+00:10:55,470 --> 00:10:59,690
+production لكن في ال lysine iron agar قولنا في حال
+
+168
+00:10:59,690 --> 00:11:03,930
+كان عندي H2S positive ماقدر احدد هل هي alkaline او
+
+169
+00:11:03,930 --> 00:11:08,230
+هل هي acidic اللي هو yellow هل هي عملت اللي هو
+
+170
+00:11:08,230 --> 00:11:12,290
+lysine decarboxylation او عملت gelicose
+
+171
+00:11:12,290 --> 00:11:17,480
+fermentation تمامهذه معناها أن البكتيريا عملت
+
+172
+00:11:17,480 --> 00:11:19,960
+جليكوز fermentation السلموني اللي عملت جليكوز
+
+173
+00:11:19,960 --> 00:11:23,080
+fermentation اتكون عندى acidic condition ال acidic
+
+174
+00:11:23,080 --> 00:11:26,600
+condition كان مهم جدا ل ال H2S production اتكون
+
+175
+00:11:26,600 --> 00:11:31,380
+عندى ال H2S gas وكان مهم جدا كمان ل ال lycine
+
+176
+00:11:31,380 --> 00:11:36,340
+decarboxylation فاتحول اللون بعدها للون ال bare
+
+177
+00:11:36,340 --> 00:11:40,580
+ball اللي هو ال alkaline لكن هان اتكون عندى عملت
+
+178
+00:11:40,580 --> 00:11:43,060
+البكتيريا جليكوز fermentation اتكون acidic
+
+179
+00:11:43,060 --> 00:11:48,410
+conditionكان مهم لإنتاج
+
+180
+00:11:48,410 --> 00:11:51,150
+الـ H2 acid production لكن مع ان البكتيريا كان
+
+181
+00:11:51,150 --> 00:11:55,610
+القدرة انها تعمل Lycine decarboxylation فعملت
+
+182
+00:11:55,610 --> 00:12:01,020
+Lycine اللي هو deaminationأو عملات ممكن كمان اللي
+
+183
+00:12:01,020 --> 00:12:08,200
+هي الـ G-Latin Deamination هذا بالنسبة لهذه
+
+184
+00:12:08,200 --> 00:12:11,360
+التفسير النتائج هنا ممكن احنا نشوف الـ triple
+
+185
+00:12:11,360 --> 00:12:15,080
+sugar iron agar alkaline أو acidic jump في الـ
+
+186
+00:12:15,080 --> 00:12:18,520
+butt jump الـ H2S gas لأنه احنا قلنا اللي يسمى D
+
+187
+00:12:18,520 --> 00:12:22,880
+-carboxylationبيصير في ظروف بتحفظ في ظروف acidic
+
+188
+00:12:22,880 --> 00:12:25,520
+condition فأي بكتيريا عاملة lysine decarboxylation
+
+189
+00:12:25,520 --> 00:12:28,540
+بالاصل هي بتكون عاملة جليكوز fermentation اتكون
+
+190
+00:12:28,540 --> 00:12:31,700
+acidic condition لازم لاقى الـ H2O acid production
+
+191
+00:12:31,700 --> 00:12:35,120
+و لازم لاقى ال lysine decarboxylation هلأ ال
+
+192
+00:12:35,120 --> 00:12:39,200
+lysine decarboxylation تمام بتكون في عندي مركب
+
+193
+00:12:39,200 --> 00:12:44,740
+قاعدي اللي هو ال cadaverin هذا هيرفعلي ال ال BH
+
+194
+00:12:44,740 --> 00:12:52,080
+وهيحول اللون الوسط للون بارفلكيف أنا بزرع اللي هو
+
+195
+00:12:52,080 --> 00:12:57,120
+بعمل stabbing للـBUT و بزرع اللي هو الـslant
+
+196
+00:12:57,120 --> 00:13:03,140
+زيجزاج هذا بالنسبة للنتاج احنا قلنا لو انا شوفت في
+
+197
+00:13:03,140 --> 00:13:08,020
+ال .. بشوف فيه اول اشي في الـBUT الـBUT لو كان في
+
+198
+00:13:08,020 --> 00:13:11,180
+عندي الـBUT purple يبقى بعرف انه و الله صار في
+
+199
+00:13:11,180 --> 00:13:14,740
+عندي lysine decarboxylation positive بما انه صار
+
+200
+00:13:14,740 --> 00:13:17,120
+في عندي lysine decarboxylation ده أكون عارفة في
+
+201
+00:13:17,120 --> 00:13:20,910
+الأصل عملة البكتيرياجليكوز fermentation لأن
+
+202
+00:13:20,910 --> 00:13:24,290
+البكتيريا عندها القدرة أن تعمل lysine
+
+203
+00:13:24,290 --> 00:13:28,630
+decarboxylation هذا بيصير في ظروف acidic condition
+
+204
+00:13:28,630 --> 00:13:31,810
+من وين بده ييجي من الجليكوز fermentation يبقى حد
+
+205
+00:13:31,810 --> 00:13:35,050
+ما أشوف في بيربول في ال بط بيربول يبقى lysine
+
+206
+00:13:35,050 --> 00:13:38,010
+decarboxylation جنبه على طول جليكوز fermentation
+
+207
+00:13:38,010 --> 00:13:44,510
+تمامبالنسبة لـ Slice Lent بما انه اتكون لون باربل
+
+208
+00:13:44,510 --> 00:13:47,650
+فيه لسلنت يبقى بدي اعرف انه البكتيريا عملت gelatin
+
+209
+00:13:47,650 --> 00:13:51,430
+deamination ممنوع انها تكون عاملة lysine
+
+210
+00:13:51,430 --> 00:13:53,590
+deamination لأنه بما انها عملت lysine
+
+211
+00:13:53,590 --> 00:13:56,630
+decarboxylation اكيد مش هتعمل lysine deamination
+
+212
+00:13:56,630 --> 00:14:02,270
+باربل و باربل قلنا الرمز هيكون K على K هن في البط
+
+213
+00:14:02,270 --> 00:14:06,760
+اكيد البكتيريا عملت glucose fermentationماشوفتش
+
+214
+00:14:06,760 --> 00:14:10,520
+اللون البربول في البط يبقى lysine decarboxylase
+
+215
+00:14:10,520 --> 00:14:15,420
+negative بالنسبة للبربول بالإسلان يبقى برضه كانت
+
+216
+00:14:15,420 --> 00:14:19,400
+lysine deamination negative يبقى هي عملت هان اللي
+
+217
+00:14:19,400 --> 00:14:23,880
+هو deamination للجيلاتين يبقى هان البكتيريا
+
+218
+00:14:23,880 --> 00:14:27,180
+ماعندها القدرة إنها تعمل ولا deamination للليسين
+
+219
+00:14:27,180 --> 00:14:31,120
+ولا decarboxylation للليسين بما إن يالو يبقى الرمز
+
+220
+00:14:31,120 --> 00:14:35,260
+A بما إن بيربول يبقى الرمز KYellow في البط يبقى
+
+221
+00:14:35,260 --> 00:14:37,840
+Gelicose fermentation positive Lycine
+
+222
+00:14:37,840 --> 00:14:42,160
+decarboxylation negative بالنسبة للإسلاند ماعندي
+
+223
+00:14:42,160 --> 00:14:46,940
+لون purple يبقى ما اتكون عندي اللي هو الجيلاتين
+
+224
+00:14:46,940 --> 00:14:52,260
+اللي بالنسبة للإسلاند اللون ال red معناه اللي هو
+
+225
+00:14:52,260 --> 00:14:56,740
+Lycine deamination positive لكن ماعملت جيلاتين
+
+226
+00:14:56,740 --> 00:14:59,600
+deamination يبقى هان اللون ال red في الإسلاند
+
+227
+00:14:59,600 --> 00:15:03,850
+معناه Lycine deamination positive يبقى Yellow Aالـ
+
+228
+00:15:03,850 --> 00:15:07,470
+Red قلنا بما أن الـ Red بقى برمز لإله بالرمز R مش
+
+229
+00:15:07,470 --> 00:15:11,750
+الرمز K هاي البكتيريا فقط عندها القدرة أنها تعمل
+
+230
+00:15:11,750 --> 00:15:14,690
+Deamination للليسينومينة عندها القدرة أنها تعمل
+
+231
+00:15:14,690 --> 00:15:18,510
+Decarboxylation للليسينومينة الـ Black precipitate
+
+232
+00:15:18,510 --> 00:15:22,710
+قلنا الـ H2S production هاي ما بقدر أشوفها أو ما
+
+233
+00:15:22,710 --> 00:15:28,010
+بقدر إذا كانت H2S production أحدد هل هي A أو هل هي
+
+234
+00:15:28,010 --> 00:15:31,700
+K هل هي Gelicose fermentation أو هل هياللي هي
+
+235
+00:15:31,700 --> 00:15:34,920
+lysine decarboxylation مع gelicose fermentation
+
+236
+00:15:34,920 --> 00:15:39,280
+لازم أعمل الفحص لحالي لازم أعمل media يكون مافيها
+
+237
+00:15:39,280 --> 00:15:44,600
+ال substrate اللي هو لل sodium thiosulfate
+
+238
+00:15:44,600 --> 00:15:50,880
+reductase طيب في هذه ال media ممنوع اشوف ممنوع
+
+239
+00:15:50,880 --> 00:15:54,580
+احنا نشوف انه النتيجة يكون في عندي lysine
+
+240
+00:15:54,580 --> 00:15:57,960
+deamination تمام lysine deamination في الإسلات
+
+241
+00:15:57,960 --> 00:16:01,710
+ممنوع اشوف اللون ال redفي الـ slant واللون ال
+
+242
+00:16:01,710 --> 00:16:06,630
+berbol في البط لأن اللون ال red في ال slant معناه
+
+243
+00:16:06,630 --> 00:16:10,830
+lycindy amination positive اللون ال berbol في البط
+
+244
+00:16:10,830 --> 00:16:13,610
+معناه lycindy carboxylation positive وإحنا قلنا
+
+245
+00:16:13,610 --> 00:16:16,510
+ممنوع البكتيريا تعمل الأثنان مع بعض يبقى اللون ال
+
+246
+00:16:16,510 --> 00:16:19,170
+red في ال slant و ال berbol بالبط ممنوع أني أنا
+
+247
+00:16:19,170 --> 00:16:27,380
+أشوفههذه تفسير النتائج هنا نلاحظ أن اللون purple
+
+248
+00:16:27,380 --> 00:16:31,880
+في الإسلاند و purple في البط يبقى K على K طبعا
+
+249
+00:16:31,880 --> 00:16:35,200
+تفسير النتائج زي ما قلنا بما أن purple و purple
+
+250
+00:16:35,200 --> 00:16:39,720
+purple في الإسلاند يبقى gelatin purple في الإسلاند
+
+251
+00:16:39,720 --> 00:16:45,900
+يبقى gelatin deamination purple في البط
+
+252
+00:16:45,900 --> 00:16:51,590
+يبقى lysine decarboxylation هنا K على Aمنلاحظ أنه
+
+253
+00:16:51,590 --> 00:16:54,790
+هنا بما أنه في الـBot A يبقى Gelicose fermentation
+
+254
+00:16:54,790 --> 00:17:01,830
+في الـslant هنا K يبقى هي في الـslant تتحول للون
+
+255
+00:17:01,830 --> 00:17:04,950
+للون Violet يبقى هي Gelatin Deamination يبقى هنا
+
+256
+00:17:04,950 --> 00:17:07,190
+البكتيريا ماعندها القدرة أن تتعمل للليسين ولا
+
+257
+00:17:07,190 --> 00:17:11,950
+Deamination ولا Decarboxylation هنا الرمز في عندي
+
+258
+00:17:11,950 --> 00:17:14,610
+Yellow في الـBot يبقى Gelicose fermentation
+
+259
+00:17:14,610 --> 00:17:17,410
+positive ليسين Decarboxylation negative في عندى
+
+260
+00:17:17,410 --> 00:17:23,280
+تشققات ممكن نضيف Gasفي عندي هان لون Red في ال ..
+
+261
+00:17:23,280 --> 00:17:25,900
+اللي هو في الـ Slant يبقى عاملة الـ Bacteria
+
+262
+00:17:25,900 --> 00:17:33,360
+Lysine D Amination هان في عندي H2S اللي هو H2S
+
+263
+00:17:33,360 --> 00:17:37,680
+لأنه اللي اتكون اللون الأسود الـ Plus هاي معناها
+
+264
+00:17:37,680 --> 00:17:41,200
+أنه في عندي تشققات نسينا نقول في ال triple sugar
+
+265
+00:17:41,200 --> 00:17:44,700
+iron agar ممكن أضيف رمز الجاز و ممكن أضيف رمز الـ
+
+266
+00:17:44,700 --> 00:17:47,880
+Plus معناه أنه والله في عندي اتكون جاز يبقى رمز
+
+267
+00:17:47,880 --> 00:17:51,950
+الـ Plusمعناه في عندي اللي هو اتكون نتيجة
+
+268
+00:17:51,950 --> 00:17:55,950
+fermentation acid with gas يبقى ال plus ممكن ارمز
+
+269
+00:17:55,950 --> 00:18:00,390
+ال plus ممكن ارمز اللي هو ال gas طيب فوق في ال
+
+270
+00:18:00,390 --> 00:18:04,530
+slant اتكون اللون اللي هو ال red يبقى صار في عندي
+
+271
+00:18:04,530 --> 00:18:10,370
+اللي هو lysine deamination طيب ليش أنا كتبت ايه؟
+
+272
+00:18:10,370 --> 00:18:13,930
+ليش أنا كتبت ايه؟ احنا قلنا في هذه الحالة بما انه
+
+273
+00:18:13,930 --> 00:18:20,320
+اتكون H2S gas ماقدر اكتب ايه ولا كامهان بقوللي في
+
+274
+00:18:20,320 --> 00:18:24,320
+هذه الحالة بما أن البكتيريا عملت ليسين D-amination
+
+275
+00:18:24,320 --> 00:18:27,500
+كانت أعرفه أكيد مش هتكون عاملة ليسين D
+
+276
+00:18:27,500 --> 00:18:31,540
+-carboxylation فبالتالي برمز للرمز A يبقى في هذه
+
+277
+00:18:31,540 --> 00:18:34,780
+الحالة انا احنا قولنا الـH2S بما أنه اتكون في الـ
+
+278
+00:18:34,780 --> 00:18:39,280
+triple في الـ lycine iron agar يبقى ما بقدر أحدد A
+
+279
+00:18:39,280 --> 00:18:43,640
+أو K لأن الـH2S production بيصير في ظروف acidic
+
+280
+00:18:43,640 --> 00:18:46,620
+condition الـA فعلا صار في انديجليكوز fermentation
+
+281
+00:18:47,230 --> 00:18:49,710
+بالنسبة للـ K في الأصل هي عملت جليكوز fermentation
+
+282
+00:18:49,710 --> 00:18:53,550
+لما بعدين عملت lysine decarboxylation لكن هنا أنا
+
+283
+00:18:53,550 --> 00:18:58,990
+كتبت A لأن الرمز الـ R اللي هو الـ R مع أنها صار
+
+284
+00:18:58,990 --> 00:19:01,650
+عندي lysine deamination لما أنها عملت lysine
+
+285
+00:19:01,650 --> 00:19:04,670
+deamination يبقى مع عملت lysine decarboxylation
+
+286
+00:19:04,670 --> 00:19:07,970
+يبقى فقط بيكون عندي اللي تحت اللي هو الجليكوز
+
+287
+00:19:07,970 --> 00:19:11,270
+fermentation هاي برضه منضيف اللي هو الـ plus R
+
+288
+00:19:11,270 --> 00:19:16,200
+الجاز عليها لأنه في عندي تشققات تحتخلصنا هيك
+
+289
+00:19:16,200 --> 00:19:20,040
+ميديات اللي هي Lycine Iron Agarni جلال الميديا
+
+290
+00:19:20,040 --> 00:19:24,280
+التانية اللي هي ال Motility Indole Ornithine MIO
+
+291
+00:19:24,280 --> 00:19:29,340
+بما أن في عندي Motility يبقى هتكون Stab at you هذه
+
+292
+00:19:29,340 --> 00:19:33,300
+الميديا مش هاكشف على ال H2S Gas هتوفر لي European
+
+293
+00:19:33,300 --> 00:19:36,860
+Condition فا من خلالها هاكشف على ال Indole وعلى ال
+
+294
+00:19:36,860 --> 00:19:39,660
+Ornithine Deamination أي ال Deamination بيصير في
+
+295
+00:19:39,660 --> 00:19:42,830
+ظروف European Conditionبالنسبة للـ Un-Aerobic
+
+296
+00:19:42,830 --> 00:19:45,030
+Condition قلنا الجليكوز fermentation والـ
+
+297
+00:19:45,030 --> 00:19:48,170
+decarboxylation بيصير في الـ Un-Aerobic Condition
+
+298
+00:19:48,170 --> 00:19:52,510
+الـ pH Indicator نفس ميديا الليسين ايرون اجاري
+
+299
+00:19:52,510 --> 00:19:58,490
+اللي هي البراموس رزال بيربال بتكون لون يلو في ال
+
+300
+00:19:58,490 --> 00:20:01,430
+acid condition في ال basil condition بتكون لون
+
+301
+00:20:01,430 --> 00:20:05,610
+بيربال فيه لنيوترال بيكون لون إجريكطيب احنا قلنا
+
+302
+00:20:05,610 --> 00:20:10,370
+في تحت لو انا شوفت لون أصفر معناه انه البكتيريا
+
+303
+00:20:10,370 --> 00:20:14,250
+عملت gelicose fermentation اتكوّن ال acid حوّل لون
+
+304
+00:20:14,250 --> 00:20:19,010
+ال ال بي اتش indicator لللون اليالو طب لو انا شوفت
+
+305
+00:20:19,010 --> 00:20:22,030
+تحت لون bearable معناه انه البكتيريا عملت
+
+306
+00:20:22,030 --> 00:20:26,250
+decarboxylation لأرنيثين اتكوّن alkaline product و
+
+307
+00:20:26,250 --> 00:20:30,650
+حولت ال media لللون bearable طب على السطح لو انا
+
+308
+00:20:30,650 --> 00:20:34,420
+ضفت ال cavex reagent يبقىالبكتيريا عندها أنزيم
+
+309
+00:20:34,420 --> 00:20:38,000
+لتريبتوفاناز جسرت لتريبتوفان اتكون اندول ارتبط مع
+
+310
+00:20:38,000 --> 00:20:42,500
+الكوفكريازيران تتكون اندي red ring طب لو كان على
+
+311
+00:20:42,500 --> 00:20:45,120
+سطح لون فيه purple معناه ان البكتيريا عملت
+
+312
+00:20:45,120 --> 00:20:48,700
+deamination لارنيثين اتكون اندي alkaline product
+
+313
+00:20:48,700 --> 00:20:52,840
+حول ال pH indicator لللون ال purple هذا بالنسبة
+
+314
+00:20:52,840 --> 00:20:57,870
+لهاي ال mediaنشوف مكونات ال media أكيد yes extract
+
+315
+00:20:57,870 --> 00:21:04,090
+كمصادر للتغذية بيبتون تمام لأن البكتيري هتعمل ال
+
+316
+00:21:04,090 --> 00:21:11,110
+ال deamination للبيبتون sorry في هذه الفقرة نمسح
+
+317
+00:21:11,110 --> 00:21:16,270
+ال ornithine deamination و نكتب بيبتون deamination
+
+318
+00:21:16,270 --> 00:21:20,830
+هان
+
+319
+00:21:20,830 --> 00:21:23,710
+بيبتون deamination مش decarboxylation
+
+320
+00:21:26,080 --> 00:21:29,500
+مش ornithine هان اللي هي ال peptone دي amination و
+
+321
+00:21:29,500 --> 00:21:33,080
+هان اللي هي برضه ال peptone دي amination تمام؟ بدي
+
+322
+00:21:33,080 --> 00:21:36,100
+أكشف في هذه ال media عن قدرة البكتيريا أنها تعمل
+
+323
+00:21:36,100 --> 00:21:38,680
+decarboxylation لل ornithine لو ماعملت
+
+324
+00:21:38,680 --> 00:21:42,120
+decarboxylation هتعمل deamination لل peptone في ال
+
+325
+00:21:42,120 --> 00:21:42,960
+European condition
+
+326
+00:21:46,330 --> 00:21:48,710
+بالنسبة لأ ال Ornithin موجود أكيد في ال media
+
+327
+00:21:48,710 --> 00:21:51,430
+لأنها أكشف عن قدرة البكتيريا أن تعمل دي
+
+328
+00:21:51,430 --> 00:21:54,650
+Carboxylation ال Dextrose موجود بكمية قليلة عشان
+
+329
+00:21:54,650 --> 00:21:58,410
+أكشف على ال fermentation الأجار بما أن فحص
+
+330
+00:21:58,410 --> 00:22:02,350
+Motility يبقى هي semi-solid media نسبة الأجار 4 من
+
+331
+00:22:02,350 --> 00:22:08,230
+10% ال pH indicator ال برامو سرزال بيربول قلنا
+
+332
+00:22:08,230 --> 00:22:13,020
+حدوده شو بتكون في ال acidic و ال basicالـ MIO
+
+333
+00:22:13,020 --> 00:22:18,880
+media تستخدم عشان اني انا افرق اللي هو ال او اكشف
+
+334
+00:22:18,880 --> 00:22:22,740
+على تلت فحوصات اللي هي في خلال one culture two
+
+335
+00:22:22,740 --> 00:22:25,920
+motility in dolly production and ornizine
+
+336
+00:22:25,920 --> 00:22:29,250
+decarboxylationبالنسبة لـ Motility قلنا بعمل
+
+337
+00:22:29,250 --> 00:22:33,390
+Stabbing في الـ Semi Solid Media باستخدام الـ
+
+338
+00:22:33,390 --> 00:22:36,750
+Straight Wire من خلال اللي هو الـ Inculating
+
+339
+00:22:36,750 --> 00:22:42,010
+Needle لو البكتيريا Motile بقولي أكيد ه will
+
+340
+00:22:42,010 --> 00:22:45,430
+migrate from the stab line by means of their
+
+341
+00:22:45,430 --> 00:22:49,610
+flagella البكتيريا هتتحرك بعيدا عن حط الزراعة
+
+342
+00:22:49,610 --> 00:22:52,890
+باستخدام الفلاجلة producing turbidity or
+
+343
+00:22:52,890 --> 00:22:54,950
+cloudiness through other media هيتكون عندك
+
+344
+00:22:54,950 --> 00:22:58,320
+turbidity و cloudiness في الميدياالـ Non-motile
+
+345
+00:22:58,320 --> 00:23:03,180
+organism هتنمو فقط على خط الزراعة فبالتالي باقي ال
+
+346
+00:23:03,180 --> 00:23:07,940
+media هتكون clear بالنسبة للفحص التاني اللي هو ال
+
+347
+00:23:07,940 --> 00:23:12,460
+Ornithin بقولي هاي ال media بدي أكشف على وجود
+
+348
+00:23:12,460 --> 00:23:15,800
+إنزيم ال Ornithin decarboxylase هل البكتيريا عندها
+
+349
+00:23:15,800 --> 00:23:20,280
+القدرة أنها تعمل decarboxylase ل Ornithin ولا لأ
+
+350
+00:23:20,280 --> 00:23:24,730
+باستخدام اللي هو ال Ornithin الموجود في ال mediaلو
+
+351
+00:23:24,730 --> 00:23:27,690
+البكتيريا عندها هذا الانزيم بقوللي هذا الانزيم
+
+352
+00:23:27,690 --> 00:23:31,450
+الارنيسين دي كربوكسيلاز نفس اللايسين دي كربوكسيلاز
+
+353
+00:23:31,450 --> 00:23:35,830
+بتنشط في ال acidic environment زي ما قلنا create
+
+354
+00:23:35,830 --> 00:23:39,570
+by the initial fermentation of the glycosic من وين
+
+355
+00:23:39,570 --> 00:23:43,310
+بده يجي ال acidic environment اللي هو ال
+
+356
+00:23:43,310 --> 00:23:46,490
+environment ال acidic information او ال acidic
+
+357
+00:23:46,490 --> 00:23:49,710
+اللي هو ال acidic condition او ال acidic
+
+358
+00:23:49,710 --> 00:23:54,030
+environment بقوللي ال acidic environment بيكونصير
+
+359
+00:23:54,030 --> 00:23:56,870
+في الأصل من خلال أن البكتيريا بالأول بتعمل
+
+360
+00:23:56,870 --> 00:24:01,050
+fermentation للجليكوز تكون عندى acidic condition
+
+361
+00:24:01,050 --> 00:24:06,110
+فبالتالي هيتحفظ لإنتاج اللى هو او لعمل ال
+
+362
+00:24:06,110 --> 00:24:09,510
+decarboxylation للأورنيكين نفس فكرة ال lycine
+
+363
+00:24:09,510 --> 00:24:13,650
+decarboxylase قولنا بيصير في acidic condition عشان
+
+364
+00:24:13,650 --> 00:24:16,830
+هيك ممكن اني انا اشوف ال H2O acid protection جنبه
+
+365
+00:24:16,830 --> 00:24:21,390
+alkaline او acidic بقولى ال amino acid لو صلوا
+
+366
+00:24:21,390 --> 00:24:22,510
+decarboxylated
+
+367
+00:24:26,500 --> 00:24:30,020
+البرودكت الناتج اللى هو البرودكت الناتج الهو
+
+368
+00:24:30,020 --> 00:24:32,520
+البرودكت الناتج الهو البرودكت الناتج الهو البرودكت
+
+369
+00:24:32,520 --> 00:24:33,020
+الناتج الهو البرودكت الناتج الهو البرودكت الناتج
+
+370
+00:24:33,020 --> 00:24:35,520
+الهو البرودكت الناتج الهو البرودكت الناتج الهو
+
+371
+00:24:35,520 --> 00:24:39,060
+البرودكت الناتج الهو البرودكت الناتج الهو
+
+372
+00:24:39,060 --> 00:24:42,680
+البرودكت
+
+373
+00:24:42,680 --> 00:24:43,500
+الناتج الهو البرودكت الناتج الهو البرودكت الناتج
+
+374
+00:24:43,500 --> 00:24:43,500
+الهو البرودكت الناتج الهو البرودكت الناتج الهو
+
+375
+00:24:43,500 --> 00:24:43,660
+البرودكت الناتج الهو البرودكت الناتج الهو البرودكت
+
+376
+00:24:43,660 --> 00:24:48,880
+الناتفي Lysine Decarboxylase في ميديا Lysine Iron
+
+377
+00:24:48,880 --> 00:24:53,580
+Agar كان المركب الناتج من ال Lysine Decarboxylase
+
+378
+00:24:53,580 --> 00:24:57,620
+الكفاديرين تمام لكن هان هذا هو المركب الناتج من
+
+379
+00:24:57,620 --> 00:25:02,670
+عملية ال ال Ornithine Decarboxylationبقول لي هيرفع
+
+380
+00:25:02,670 --> 00:25:05,650
+الـ pH هيتكوّن عندي alkaline product فبالتالي
+
+381
+00:25:05,650 --> 00:25:09,690
+هيتحول لون ال media للون dark purple لو البكتيريا
+
+382
+00:25:09,690 --> 00:25:11,930
+ما عندها القدرة أو ما عندها ال enzyme في الأصل
+
+383
+00:25:11,930 --> 00:25:16,710
+فبالتالي هيظل أسدك الناتج من عملية ال fermentation
+
+384
+00:25:16,710 --> 00:25:20,970
+لجليكوز فهيتحول لون ال media في اللي هو البطم في
+
+385
+00:25:20,970 --> 00:25:22,750
+تحت هيتحول للون أصفر
+
+386
+00:25:27,250 --> 00:25:30,130
+أخر اشي فحص الاندول باكشف عن وجود اللي هو الـ
+
+387
+00:25:30,130 --> 00:25:33,670
+tryptophan الـ tryptophan موجود في الميديا في اللي
+
+388
+00:25:33,670 --> 00:25:38,510
+هو ال بيبتون تمام بالنسبة لل positive result
+
+389
+00:25:38,510 --> 00:25:43,630
+هيتحول لون اللي هو في ال على السطح red color اللي
+
+390
+00:25:43,630 --> 00:25:46,910
+هو ال cherry red color هذا معناه أن البكتيريا
+
+391
+00:25:46,910 --> 00:25:49,530
+عندها أنزيم لـ tryptophan بعد إضافة اللي هو
+
+392
+00:25:49,530 --> 00:25:53,130
+الكوفكس رياجان رياجان لكن ال negative result مش
+
+393
+00:25:53,130 --> 00:26:00,430
+هيتغير أي لونطب نيجي للنتائج بس
+
+394
+00:26:00,430 --> 00:26:07,190
+بالنسبة للنتائج ها يندي تلت نتائج لكن طبعا هن واحد
+
+395
+00:26:07,190 --> 00:26:10,230
+و واحد هي نفس الشيء لكن هن بعد إضافة ال govx
+
+396
+00:26:10,230 --> 00:26:16,370
+reagent هن اتنين و هن تلت نيجي بدنا أول شئ اللي هو
+
+397
+00:26:16,370 --> 00:26:21,870
+نكشف أو نقرأ نتيجة فحص ال motility تمام بعدين هقرأ
+
+398
+00:26:21,870 --> 00:26:27,490
+نتيجة الالـ Endol أو الـ Decarboxylase اللي هو الـ
+
+399
+00:26:27,490 --> 00:26:30,810
+Ornithine Decarboxylase بعدين هقرأ اللي هو هضيف ال
+
+400
+00:26:30,810 --> 00:26:35,410
+Kovacs reagent و هقرأ نتيجة اللي هو ال Endol test
+
+401
+00:26:35,410 --> 00:26:43,190
+بالنسبة لأول اللي هو ال tube أول tube بالنسبة لفحص
+
+402
+00:26:43,190 --> 00:26:47,990
+ال motility اللي هي non motile بنلاحظ أنه على خط
+
+403
+00:26:47,990 --> 00:26:54,440
+الزراعة بالنسبة لل Ornithine Decarboxylase قلناتحت
+
+404
+00:26:54,440 --> 00:27:00,020
+اتكون في على الاسفل اللي هو في ال in the bottom في
+
+405
+00:27:00,020 --> 00:27:03,640
+اللي هو الان ال condition صار في عندي لون أصفر
+
+406
+00:27:03,640 --> 00:27:07,000
+يبقى معناه جيليكوز fermentation positive ما صار في
+
+407
+00:27:07,000 --> 00:27:11,920
+عندي ornithine اللي هو دي كربوكسيلاز كانت negative
+
+408
+00:27:11,920 --> 00:27:15,180
+يبقى high ornithine دي كربوكسيلاز negative جيليكوز
+
+409
+00:27:15,180 --> 00:27:21,380
+fermentation positiveهنا بعد ما اضفت الكوفاكس
+
+410
+00:27:21,380 --> 00:27:26,420
+رياجان تحولت اللون الحلقة للون أحمر يبقى اندال
+
+411
+00:27:26,420 --> 00:27:33,200
+بوزيتف هنا في ND ما عملت البكتيريا ديكاربوكسلاشن
+
+412
+00:27:33,200 --> 00:27:37,920
+لأرنسين عملت ديأميناشن للبيبطون عشان هيك اتحول
+
+413
+00:27:37,920 --> 00:27:42,560
+اللون اللي على السطح للون violet للون بيربول dark
+
+414
+00:27:42,560 --> 00:27:47,330
+purpleبعد إضافة الـ Covax reagent نلاحظ أن اللون
+
+415
+00:27:47,330 --> 00:27:52,070
+البربل على السطح فيه اختفى لماذا؟ لأن الـ Covax
+
+416
+00:27:52,070 --> 00:27:56,690
+reagent أحد مكوناته الـ HCl إضافة الـ HCl يتحول
+
+417
+00:27:56,690 --> 00:28:00,230
+لون الـ media للون بما أن في عندي BH Indicator
+
+418
+00:28:00,230 --> 00:28:04,910
+لبرومو سيرزال بيربل موجود في ال acid condition لون
+
+419
+00:28:04,910 --> 00:28:10,310
+يلو فبالتالي اتحول للون يلو على السطحتغير اللان
+
+420
+00:28:10,310 --> 00:28:13,630
+اللى على السطح لللان يلو بعد إضافة الـ COVAX
+
+421
+00:28:13,630 --> 00:28:18,010
+reagent اتحول اللان للان يلو لأن الـ SCL عندى
+
+422
+00:28:18,010 --> 00:28:24,110
+acidic فبالتالي اتحول اللان للان يلو طيب نيجى لهاي
+
+423
+00:28:24,110 --> 00:28:29,970
+ال media أو لتانى فحص نيجى اللى اللى هو في البطام
+
+424
+00:28:29,970 --> 00:28:34,910
+بنحكى ornithine decarboxylase positive بما أنه صار
+
+425
+00:28:34,910 --> 00:28:38,200
+في عندي ornithine decarboxylasePositive يبقى أكيد
+
+426
+00:28:38,200 --> 00:28:41,160
+في الأصل كانت عندي جليكوز fermentation positive
+
+427
+00:28:41,160 --> 00:28:44,180
+يبقى هي جليكوز fermentation positive Ornithine
+
+428
+00:28:44,180 --> 00:28:51,140
+decarboxylase positive تمام بالنسبة لاللي هو ال
+
+429
+00:28:51,140 --> 00:28:54,780
+motility ال motility ماقدر أكشف على ال motility زي
+
+430
+00:28:54,780 --> 00:28:59,020
+بحث ال H2S production أحنا قلنا في حاجة عن عندي
+
+431
+00:28:59,020 --> 00:29:02,020
+H2S production ماقدر أكشف على ال motility في ميدية
+
+432
+00:29:02,020 --> 00:29:06,040
+ال SIM وهنا نفس الفكرة ماقدر أكشف على ال motility
+
+433
+00:29:06,040 --> 00:29:11,910
+في ميديةالـ MIO في حال كانت عملة البكتيريا أو الـ
+
+434
+00:29:11,910 --> 00:29:16,150
+Nitin-D-Carboxylase لازم أعمل الفحص لحال في ميديا
+
+435
+00:29:16,150 --> 00:29:20,550
+الـ Nutrient Growth مع أجار بنسبة 4 من 10% تمام
+
+436
+00:29:20,550 --> 00:29:24,730
+هذا بالنسبة لهذه الميديا ضيفت اللي هو الـ COVAX
+
+437
+00:29:24,730 --> 00:29:28,610
+-Reagent يبقى الـ Indole Negative ملاحظ إنه اللون
+
+438
+00:29:28,610 --> 00:29:31,630
+صار لونه أصفر لأن أحنا قلنا الـ COVAX-Reagent في
+
+439
+00:29:31,630 --> 00:29:37,540
+HCL هذه الميديا الـ Motility بنكتب unknownوها نفس
+
+440
+00:29:37,540 --> 00:29:39,620
+الشيء نكتب الـ motility unknown ornithine
+
+441
+00:29:39,620 --> 00:29:42,640
+decarboxylase positive بما أنه عملت decarboxylase
+
+442
+00:29:42,640 --> 00:29:45,620
+لالورنثين يبقى هي جليكوز في الأصل fermentation
+
+443
+00:29:45,620 --> 00:29:52,280
+positive ضيفت ال Kovacs reaction اتكون عندي حلقة
+
+444
+00:29:52,280 --> 00:29:56,600
+حمرة يبقى هي indole positive يبقى ال motility
+
+445
+00:29:56,600 --> 00:29:59,900
+unknown ornithine decarboxylase positive بما أنه
+
+446
+00:29:59,900 --> 00:30:01,920
+عملت هاك يبقى هي في الأصل جليكوز fermentation
+
+447
+00:30:01,920 --> 00:30:05,100
+positive لأنه احنا قلنا ال decarboxylation بيصير
+
+448
+00:30:05,100 --> 00:30:08,770
+في acidic environmentالـ Indol بما أنه اتحول أو
+
+449
+00:30:08,770 --> 00:30:12,170
+اتكون حلقة حمرا يبقى هي positive ليش تغير لونها
+
+450
+00:30:12,170 --> 00:30:15,310
+للون أصفر نفس الفكرة الـ COVAX-REACTION بتكون من
+
+451
+00:30:15,310 --> 00:30:20,510
+HCl هيتحول الوسط لوسط حمضي و هيحول لون الـ pH
+
+452
+00:30:20,510 --> 00:30:25,470
+Indicator للون Yellow هيك بنكون خلصنا اللي هو
+
+453
+00:30:25,470 --> 00:30:29,730
+Chapter Single Media Multiple Test يعطيكم العافية
+
+454
+00:30:29,730 --> 00:30:31,990
+والسلام عليكم ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/a4LrdmbI9CQ_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/a4LrdmbI9CQ_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..c8a2056f9ef6f89102cff87957807476e2dc51c0
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/a4LrdmbI9CQ_postprocess.srt
@@ -0,0 +1,1768 @@
+1
+00:00:00,370 --> 00:00:02,690
+في هاي ال slide هنتكلم عن بعض النقاط المهمة
+
+2
+00:00:02,690 --> 00:00:06,190
+بالنسبة لل light compound microscope أول شئ ال
+
+3
+00:00:06,190 --> 00:00:09,210
+magnification ال magnification هي قوة التكبير
+
+4
+00:00:09,210 --> 00:00:13,490
+relative enlargement of the specimen هو قوة تكبير
+
+5
+00:00:13,490 --> 00:00:17,130
+العين أو الشريحة حتى أحصل على ال total
+
+6
+00:00:17,130 --> 00:00:21,530
+magnification بضرب ال Ibs magnification ضرب ال
+
+7
+00:00:21,530 --> 00:00:25,190
+objective magnification قوة التكبير العدسة العينية
+
+8
+00:00:25,190 --> 00:00:29,250
+اللي هي الocular lens magnification اللي هي ثابت
+
+9
+00:00:29,250 --> 00:00:33,590
+10Xدرب قوة تكبير العدسة الشيئية اللي أنا استخدمتها
+
+10
+00:00:33,590 --> 00:00:40,150
+فيها عندي أربع عدسات شيئية 4x 10x 40x 100x حسب أنا
+
+11
+00:00:40,150 --> 00:00:43,770
+شو استخدمت بضربها بقوة تكبير العدسة العينية حتى
+
+12
+00:00:43,770 --> 00:00:47,510
+أحصل على ال total magnification the image seen by
+
+13
+00:00:47,510 --> 00:00:50,430
+the eye through a compound microscope is termed
+
+14
+00:00:50,430 --> 00:00:54,580
+the virtualimage بقولك الصورة اللي انا بحصل عليها
+
+15
+00:00:54,580 --> 00:00:58,080
+من خلال ال light compound microscope بوصفها بإنها
+
+16
+00:00:58,080 --> 00:01:02,940
+virtual image اش يعني virtual يعني افتراضية في
+
+17
+00:01:02,940 --> 00:01:07,260
+الوضع الطبيعي انا لو جبت حشرة بشوفها ب3 أبعاد طول
+
+18
+00:01:07,260 --> 00:01:10,420
+و عرض و ارتفاع لكن انا لو روحت جبت هذه الحشرة و
+
+19
+00:01:10,420 --> 00:01:14,740
+حطيتها تحت ال microscope عشان اشوفهاهشوف فقط طول و
+
+20
+00:01:14,740 --> 00:01:18,720
+عرض بالتالي هي صورة افتراضية صورة virtual مش
+
+21
+00:01:18,720 --> 00:01:24,600
+حقيقية ماشوفتها بيه الوضع الطبيعي يبقى اول اشي
+
+22
+00:01:24,600 --> 00:01:28,640
+بحكي ان هي عبارة عن virtual image صورة افتراضية
+
+23
+00:01:28,640 --> 00:01:33,060
+and is upside شو يعني upside لو انا جبت هاي الحشة
+
+24
+00:01:33,060 --> 00:01:36,600
+لو حطتها تحت ال microscope فقط هشوف اللي على السطح
+
+25
+00:01:37,030 --> 00:01:40,270
+مش هشوف اشي من تحت لو بدي اشوف التفاصيل بروح
+
+26
+00:01:40,270 --> 00:01:46,130
+بقطحها زي ما بنقطع في معمل الانسجار and reversed
+
+27
+00:01:46,130 --> 00:01:50,970
+اش يعني reversed يعني معكوسة الضوء طبعا لما يمر
+
+28
+00:01:50,970 --> 00:01:54,350
+هيصيرله انكسار لما احنا نشوف حالنا على المرايا
+
+29
+00:01:54,350 --> 00:01:57,790
+نشوف الصورة بتكون معكوسة لأن الضوء لما يمر عبر
+
+30
+00:01:57,790 --> 00:02:01,510
+الزجاج هينكسر و العدسات اللي موجودة في ال
+
+31
+00:02:01,510 --> 00:02:05,670
+microscope مصموعة من الزجاج بالتالي الضوء هيمر
+
+32
+00:02:05,900 --> 00:02:10,260
+وينكسر ونشوف صورة معكوسة يبقى الصورة اللي أنا
+
+33
+00:02:10,260 --> 00:02:13,760
+بشوفها بـ light compound microscope virtual صورة
+
+34
+00:02:13,760 --> 00:02:18,000
+افتراضية and upside اللي هي فقط بشوف اللي على
+
+35
+00:02:18,000 --> 00:02:28,060
+السطح and reverse بتكون معكوسة total
+
+36
+00:02:28,060 --> 00:02:31,120
+magnification اتكلمنا حتى احصل عليه Ibs
+
+37
+00:02:31,120 --> 00:02:35,740
+magnification ضرب objective magnificationهزكلك انه
+
+38
+00:02:35,740 --> 00:02:39,800
+طبعا العدسة العينية كلها ال magnification تبعها
+
+39
+00:02:39,800 --> 00:02:44,820
+10x العدسات الشيئية انا فيها 4x بسميها scanning
+
+40
+00:02:44,820 --> 00:02:48,660
+objective ايش يعني scanning يعني ماسحة انا من
+
+41
+00:02:48,660 --> 00:02:53,660
+خلالها بشوف كل الشريحة كل slide بلقي نظرة على كل
+
+42
+00:02:53,660 --> 00:02:57,640
+slide لو انا بدي احصل على ال total magnification
+
+43
+00:02:57,640 --> 00:03:02,560
+من ال 4x objective ال total magnification يكون 40
+
+44
+00:03:03,060 --> 00:03:07,560
+بالنسبة لو استخدمت 10x objective بحصل على 100x
+
+45
+00:03:07,560 --> 00:03:11,040
+total magnification الاسم التاني لأ ال 10x
+
+46
+00:03:11,040 --> 00:03:21,300
+objective اللي هي ال law power لو
+
+47
+00:03:21,300 --> 00:03:25,220
+أنا استخدمت 40x objective بحصل على total
+
+48
+00:03:25,220 --> 00:03:29,720
+magnification 400x الاسم التاني لأ ال 40x
+
+49
+00:03:29,720 --> 00:03:31,520
+objective ال high power
+
+50
+00:03:38,680 --> 00:03:43,780
+يبقى الـ 4X اسمها scanning objective الـ 10X
+
+51
+00:03:43,780 --> 00:03:49,980
+بسميها low power و الـ 40X بسميها الـ high power
+
+52
+00:03:49,980 --> 00:03:55,960
+في عندي أنا الـ 100X الـ
+
+53
+00:03:55,960 --> 00:04:04,790
+100X objective لو أنا بدي أضربها بالـ 10Xاللي هي
+
+54
+00:04:04,790 --> 00:04:10,290
+الـ I piece بحصل على Total Magnification بـ 1000X
+
+55
+00:04:10,290 --> 00:04:15,050
+Total Magnification بالنسبة لـ 100X بسميها Oil
+
+56
+00:04:15,050 --> 00:04:22,630
+Emergent تمام،
+
+57
+00:04:22,630 --> 00:04:26,890
+الـ 100X بسميها Oil Emergent الـ 40X بسميها الـ
+
+58
+00:04:26,890 --> 00:04:30,680
+High Power الـ 10X بسميها الـ Low Powerالـ 4X ده
+
+59
+00:04:30,680 --> 00:04:33,720
+هسميها الـ Scanning Power هيك أنا حصلت على الـ
+
+60
+00:04:33,720 --> 00:04:40,180
+Total Magnification خلصنا
+
+61
+00:04:40,180 --> 00:04:43,140
+من الـ Magnification ومن الـ Total Magnification
+
+62
+00:04:43,140 --> 00:04:46,920
+في أنا عندي Slide اللي هي الـ Numerical Aperture
+
+63
+00:04:46,920 --> 00:04:49,960
+لكن قبل ما أتكلم على الـ Numerical Aperture بدي
+
+64
+00:04:49,960 --> 00:04:54,540
+أتكلم عن الـ Resolution كثير حكينا في الـ
+
+65
+00:04:54,540 --> 00:04:59,190
+Resolution لكن بدنا نعرفه بشكل علميالـ Resolution
+
+66
+00:04:59,190 --> 00:05:03,790
+it is the ability of microscope to distinguish two
+
+67
+00:05:03,790 --> 00:05:08,290
+points يقولك هو قدرة ال microscope أو العدسة أنه
+
+68
+00:05:08,290 --> 00:05:12,930
+يميز بين نقطتين نكمل التعريف أن هدول النقطتين
+
+69
+00:05:12,930 --> 00:05:18,130
+يكونوا المسافة بينهم قليلة يبقى that how small
+
+70
+00:05:18,130 --> 00:05:25,910
+distance between it نحكي that how small
+
+71
+00:05:31,200 --> 00:05:38,160
+distance between them
+
+72
+00:05:38,160 --> 00:05:43,540
+هو قدرة الميكروسكوب أو العدسة يميز بين نقطتين
+
+73
+00:05:43,540 --> 00:05:45,840
+المسافة بينهم قليلة
+
+74
+00:05:53,680 --> 00:05:57,080
+The smaller the distance between the two specific
+
+75
+00:05:57,080 --> 00:06:00,940
+objectives that can be distinguished apart, the
+
+76
+00:06:00,940 --> 00:06:03,960
+greater the resolution power of the microscope
+
+77
+00:06:03,960 --> 00:06:09,060
+وقولّك كل ما كانت المسافة ما بين جثمان مختلفان
+
+78
+00:06:09,060 --> 00:06:12,800
+وانا قدرت اميز بين هدول الجثمان كل ما كانت المسافة
+
+79
+00:06:12,800 --> 00:06:16,740
+الصغيرة وانا قدرت اميز بين هدول الجثمان كل ما كان
+
+80
+00:06:16,740 --> 00:06:20,670
+ال resolution power لهذا ال microscope عالييعني لو
+
+81
+00:06:20,670 --> 00:06:23,630
+كان الـ Microscope الـ Resolution تبعه عالي معناه
+
+82
+00:06:23,630 --> 00:06:27,290
+أنا هقدر أميز بين نقطتين مختلفتين المسافة بينهم
+
+83
+00:06:27,290 --> 00:06:31,070
+كتير قليلة و أحكي إنه والله هذا جسم X و هذا جسم Y
+
+84
+00:06:31,070 --> 00:06:34,790
+برغم إن المسافة كتير قليلة بينهم لكن لو كان الـ
+
+85
+00:06:34,790 --> 00:06:38,230
+Resolution لأ الـ Microscope أو العدسة كتير قليل
+
+86
+00:06:38,230 --> 00:06:41,930
+النقطتين هدول هيبينوا كأنهم جسم واحد و هيكونوا فوق
+
+87
+00:06:41,930 --> 00:06:45,090
+بعض فبالتالي الـ Resolution لأ الـ Microscope
+
+88
+00:06:45,090 --> 00:06:50,590
+هيكون قليلThe larger enumerical aperture قبل ما
+
+89
+00:06:50,590 --> 00:06:55,690
+نتكلم عن هذه الجملة هنعرف لـ numerical aperture
+
+90
+00:06:55,690 --> 00:07:09,110
+The
+
+91
+00:07:09,110 --> 00:07:12,790
+enumerical aperture The amount of light reaching
+
+92
+00:07:12,790 --> 00:07:17,090
+the specimen هو كمية الضوء الواصلة للعينةAs
+
+93
+00:07:17,090 --> 00:07:20,090
+numerical aperture increases, the resolution
+
+94
+00:07:20,090 --> 00:07:23,910
+increases بقولّك كل ما زادت كمية الضوء اللي واصلة
+
+95
+00:07:23,910 --> 00:07:27,390
+للعينة كل ما كان الـ Resolution للـ Microscope أو
+
+96
+00:07:27,390 --> 00:07:32,930
+العدسة كبيرة احنا قلنا لو انت كنت في 10X او 4X جبت
+
+97
+00:07:32,930 --> 00:07:35,950
+ال field و روحتي على ال high power 40X بتلاقي
+
+98
+00:07:35,950 --> 00:07:40,890
+الصورة مغبشة وقولنا عشان انا تكون الصورة واضحة
+
+99
+00:07:40,890 --> 00:07:44,530
+بروح اتحكم بالـ Fine Adjustmentوقلت في بعض الأشياء
+
+100
+00:07:44,530 --> 00:07:48,170
+كمان اللي ممكن أتحكم فيها اللي هنتكلم عنها من ضمن
+
+101
+00:07:48,170 --> 00:07:51,870
+الأمور التانية اللي أنا بتحكم فيها كمية الضوء
+
+102
+00:07:51,870 --> 00:07:56,750
+المرة اللي هي الـ numerical aperture طيب أنا كيف
+
+103
+00:07:56,750 --> 00:08:00,890
+بدي أتحكم بالـ numerical aperture؟ كيف بدي أتحكم
+
+104
+00:08:00,890 --> 00:08:04,650
+بكمية الضوء المرة خلال العينة؟ اتكلمنات أجزاء
+
+105
+00:08:04,650 --> 00:08:08,930
+الميكروسكوفي عندي إشي اسمه diaphragم هذا من خلاله
+
+106
+00:08:08,930 --> 00:08:13,340
+أنا بتحكم بكمية الضوء المرة خلال العينةفبروح انا
+
+107
+00:08:13,340 --> 00:08:17,440
+عشان ازود ال numerical aperture بفتح ال diaphragm
+
+108
+00:08:17,440 --> 00:08:22,580
+شوية عشان انا عندى ايزية ال resolution تمام من
+
+109
+00:08:22,580 --> 00:08:26,640
+خلال هاي الجملة هنستنتج علاقة مهمة ان كل ما زاد ال
+
+110
+00:08:26,640 --> 00:08:30,920
+magnification كل ما زادت قوة التكبير كل ماحتجت
+
+111
+00:08:30,920 --> 00:08:36,290
+كمية الضوء اكتر حتى انها تصل للعينةبالتالي الـ
+
+112
+00:08:36,290 --> 00:08:40,350
+Resolution للـ Microscope أو العدسة بيكون كتير
+
+113
+00:08:40,350 --> 00:08:45,750
+اكتر كتير بيزيد أو بيكون كتير تمام؟ اللي بيأكد
+
+114
+00:08:45,750 --> 00:08:49,450
+الكلام انه انا لما كنت في 10X وانتقلت على الـ High
+
+115
+00:08:49,450 --> 00:08:53,070
+Power للـ 40X احتجت كمية ضوء اكتر روحت فتحت
+
+116
+00:08:53,070 --> 00:08:57,310
+Diagram الـ Diaphragm عشان يصل ضوء اكتر للعينة
+
+117
+00:08:57,310 --> 00:09:00,190
+بالتالي الـ Resolution زاد شو يعني الـ Resolution
+
+118
+00:09:00,190 --> 00:09:03,210
+زاد؟ يعني اقدرت اميز بين جثمان المسافة بينهم
+
+119
+00:09:03,210 --> 00:09:07,930
+الكتير قليلةلكن لو أنا كنت على 4X أو على 10X
+
+120
+00:09:07,930 --> 00:09:12,790
+مابحتاش كمية الضوء كبيرة وهذا كتير طالبات و طلاب
+
+121
+00:09:12,790 --> 00:09:16,530
+لما يروح يجيبوا ال field على 4X و 10X بنسوا يسكوا
+
+122
+00:09:16,530 --> 00:09:19,590
+ال diaphragm يصيروا يطلعوا كلستاج و ينزلوا و يحكوا
+
+123
+00:09:19,590 --> 00:09:23,350
+انهم مالقوا ال field لكن هو مش مالقوا ال field
+
+124
+00:09:23,350 --> 00:09:28,610
+لازم احنا نسكر ال diaphragm على عدسة 4 و 10 لأن
+
+125
+00:09:28,610 --> 00:09:31,670
+قلنا في علاقة تربية بين ال magnification و المباني
+
+126
+00:09:32,160 --> 00:09:37,600
+الـ Numerical Aperture أنا بدي الكنة العدسة أربعة
+
+127
+00:09:37,600 --> 00:09:42,000
+X قوة تتكبير كتير قليلة فبالتالي ما بحتاج كمية ضوء
+
+128
+00:09:42,000 --> 00:09:46,220
+فلازم يتسكر الديافراج نيجي لها العلاقة الرياضية
+
+129
+00:09:46,220 --> 00:09:49,980
+الـ Numerical Aperture بيقولك بيساوي الـ Angular
+
+130
+00:09:49,980 --> 00:09:54,300
+Aperture ضرب الـ Refractive Index الـ Angular
+
+131
+00:09:54,300 --> 00:09:57,840
+Aperture اللي هي الـ Sinθ ضرب الـ Refractive Index
+
+132
+00:09:57,840 --> 00:10:04,340
+هو الـ Nمايبقاش هي θ هي عبارة عن زاوية الانكسار لو
+
+133
+00:10:04,340 --> 00:10:15,940
+أنا عندي هاي ال slide و مرضو عبر ال slide هاي
+
+134
+00:10:15,940 --> 00:10:23,200
+ال slide و مرضو عبر هاي ال slide الضوء لما يمر عبر
+
+135
+00:10:23,200 --> 00:10:29,870
+ال slide هينكسر بزاوية هاي الزاويةهي عبارة عن θ
+
+136
+00:10:29,870 --> 00:10:33,970
+«زاوية الانكسار» هي θ بعوض فيها في «unrefractive
+
+137
+00:10:33,970 --> 00:10:38,510
+index» ومعامل انكسار الوصف الموجود ما بين الـ
+
+138
+00:10:38,510 --> 00:10:41,870
+«slide» وما بين العدسة الشيئية اللي هنتكلم فيه
+
+139
+00:10:41,870 --> 00:10:45,950
+بآخر المعبر الـ numerical aperture بيقولك بيتمد
+
+140
+00:10:45,950 --> 00:10:50,770
+على الـ radius of lens بيتمد على نصف قطر العدسة
+
+141
+00:10:50,770 --> 00:10:54,510
+والعلاقة ما بين الـ numerical aperture وradius
+
+142
+00:10:54,510 --> 00:10:56,890
+علاقة عكسية، تمام؟
+
+143
+00:11:00,010 --> 00:11:07,370
+نرجع لـ slide الـ «high» «The larger numerical
+
+144
+00:11:07,370 --> 00:11:11,750
+aperture, the smaller the dissolvable distance and
+
+145
+00:11:11,750 --> 00:11:14,990
+hence the more efficient the resolution power»
+
+146
+00:11:17,070 --> 00:11:20,290
+محتاجة كمية ضوء اكتر كل ما زاد ال numerical
+
+147
+00:11:20,290 --> 00:11:24,830
+aperture كل ما قدرت اميز بين نقطتان المسافة بينهم
+
+148
+00:11:24,830 --> 00:11:27,870
+اكتر قليلة بيكون بالتالي بيكون ال resolution .. ال
+
+149
+00:11:27,870 --> 00:11:32,110
+resolution لأ ال microscope او العدسة اكتر عالي
+
+150
+00:11:32,110 --> 00:11:35,190
+فيعرفنا العلاقة الرياضية عشان نفهم العلاقة
+
+151
+00:11:35,190 --> 00:11:35,870
+الرياضية
+
+152
+00:11:48,610 --> 00:11:55,250
+هنا نكتبها مكتوب
+
+153
+00:11:55,250 --> 00:12:07,930
+في عندي R اللي هو R
+
+154
+00:12:07,930 --> 00:12:16,150
+بساوي ال .. في عندي رقم مكتوب
+
+155
+00:12:45,120 --> 00:12:48,500
+الرقم درب ال web links على ال numerical aperture
+
+156
+00:12:57,620 --> 00:13:04,060
+هذه هي العلاقة الرياضية خلّينا نحكي عن كل جزء بهذه
+
+157
+00:13:04,060 --> 00:13:08,680
+العلاقة R هي عبارة عن مقياس أصغر مسافة قابلة
+
+158
+00:13:08,680 --> 00:13:13,900
+للتمييز بين نقطتين R هي مقياس يعبر عن distance عن
+
+159
+00:13:13,900 --> 00:13:17,820
+مسافة أصغر مسافة قابلة للتمييز بين نقطتين طبعا هذا
+
+160
+00:13:17,820 --> 00:13:22,020
+ثابت ال wavelength هو الطول الموجي طبعا أحنا قلنا
+
+161
+00:13:22,020 --> 00:13:26,160
+أن الضوء يمر عبر العينةفأنا سوء طول الموج اللي هدق
+
+162
+00:13:26,160 --> 00:13:29,680
+الضوء اللي بمر عبر العينة بعود عنه في هذا القانون
+
+163
+00:13:29,680 --> 00:13:33,880
+الـ numerical aperture هي كمية الضوء اللي مار عبر
+
+164
+00:13:33,880 --> 00:13:38,580
+العينة طيب، ان هاي العلاقة أنا بستنتج ان في عندي
+
+165
+00:13:38,580 --> 00:13:43,020
+أو قبل ما نحكي عن هاي العلاقة هلأ احنا قولنا كل ما
+
+166
+00:13:43,020 --> 00:13:48,460
+زاد ال magnification كل ما احتجت كمية الضوء أكتر
+
+167
+00:13:48,460 --> 00:13:52,610
+كل ما زاد ال magnification كل ما احتجتالكمية الضوء
+
+168
+00:13:52,610 --> 00:13:57,350
+أكتر اللي هو الـ numerical aperture أكتر فبالتالي
+
+169
+00:13:57,350 --> 00:14:02,570
+كل ما كان الـ resolution لأ الميكروسكوب كتير عالي
+
+170
+00:14:02,570 --> 00:14:07,250
+فبالتالي اقدرت اميز بين نقطتين المسافة بينهم كتير
+
+171
+00:14:07,250 --> 00:14:12,430
+قليلة يبقى R هي المسافة اللي بين النقطتين كتير
+
+172
+00:14:12,430 --> 00:14:16,910
+قليلة اقدرت اميز بينهم وكان كمية الضوء عالية و ال
+
+173
+00:14:16,910 --> 00:14:20,010
+resolution عالية يبقى لو بنكمل القانون عشان تكون
+
+174
+00:14:20,010 --> 00:14:26,070
+الأمور واضحة أكتربنكتب أن الـ R هي مسافة بين
+
+175
+00:14:26,070 --> 00:14:35,550
+نقطتين وهي معكوث الـ resolution يبقى
+
+176
+00:14:35,550 --> 00:14:44,890
+الواحد
+
+177
+00:14:44,890 --> 00:14:50,750
+و الـ
+
+178
+00:14:50,750 --> 00:14:51,270
+resolution
+
+179
+00:15:21,890 --> 00:15:25,850
+يبقى كل ما زاد الـ Magnification كل ما احتجت كمية
+
+180
+00:15:25,850 --> 00:15:30,910
+الضوء أكتر فبالتالي كل ما كانت قوة الـ Resolution
+
+181
+00:15:30,910 --> 00:15:35,790
+دقة الميكروسكوب أو العدسة أكتر فمعناها إيش يعني
+
+182
+00:15:35,790 --> 00:15:39,110
+الـ Resolution يعني اقدر اتميز بين نقطتين المسافة
+
+183
+00:15:39,110 --> 00:15:44,250
+بينهم كثير قليلاً من هذه العلاقة نستنتج علاقة أخرى
+
+184
+00:15:44,250 --> 00:15:47,270
+إنه ما بين الـ Resolution وما بين طول الموج اللي
+
+185
+00:15:47,270 --> 00:15:56,720
+هي علاقة عكسيةكل ما كان الطول الموجي اكتير قليل كل
+
+186
+00:15:56,720 --> 00:16:01,840
+ما كان الطول الموجي لضوء الماء عبر العين القصير كل
+
+187
+00:16:01,840 --> 00:16:05,760
+ما كان الـ Resolution عالي وقدرت اميز بين نقطتان
+
+188
+00:16:05,760 --> 00:16:10,400
+المسافة بينهم اكتر قليلة تمام؟ هذا بالنسبة للعلاقة
+
+189
+00:16:10,400 --> 00:16:14,600
+الرياضية المضحة بتمنى تكون واضحة كل الأمور تمام
+
+190
+00:16:22,560 --> 00:16:26,220
+الـ «depth of focus» معرفة ليها «thickness of
+
+191
+00:16:26,220 --> 00:16:30,460
+field» «سمك المجال» اللي هو عمق المسافة أو عمق
+
+192
+00:16:30,460 --> 00:16:34,760
+الرؤية معرفة ليها «thickness of field» اللي هو سمك
+
+193
+00:16:34,760 --> 00:16:39,080
+المجال «as magnification increases depth of focus
+
+194
+00:16:39,080 --> 00:16:43,140
+increases» إيش يعني سمك المجال أو سمك الـ field؟
+
+195
+00:16:43,140 --> 00:16:46,400
+معناه لو كان أنا عندي كتير طبقات لو عندي عينة فيها
+
+196
+00:16:46,400 --> 00:16:50,400
+كتير طبقات أنا
+
+197
+00:16:50,400 --> 00:16:55,620
+ماعملتلها تقطيعكانت العينات تتكون من عدة طبقات
+
+198
+00:16:55,620 --> 00:17:01,080
+وعينة أخرى كانت تتكون من طبقتين كل ما كانت الطبقات
+
+199
+00:17:01,080 --> 00:17:04,960
+أقل في هذه الناحية كل ما كان يعني ال depth of
+
+200
+00:17:04,960 --> 00:17:09,210
+focus كتير قليلفبالتالي بيكون الـ Resolution وضوح
+
+201
+00:17:09,210 --> 00:17:12,470
+الصورة في المنطقة اللي بيكون عندها الطبقات الأقل
+
+202
+00:17:12,470 --> 00:17:16,710
+بتكون الـ Resolution عالية وبالتالي بتحتاج قوة
+
+203
+00:17:16,710 --> 00:17:20,870
+تكبير عالية يبقى كل ما كانت الطبقات أقل يعني الـ
+
+204
+00:17:20,870 --> 00:17:23,850
+Depth of Focus كان أليل كل ما كان الـ Resolution
+
+205
+00:17:23,850 --> 00:17:26,830
+أكيد لما تكون الطبقات أليلة هيكون وضوح الصورة أكتر
+
+206
+00:17:26,830 --> 00:17:29,890
+الـ Resolution أكتر فبالتالي الـ Magnification
+
+207
+00:17:29,890 --> 00:17:32,470
+بيزيد يبقى العلاقة ما بين الـ Depth of Focus
+
+208
+00:17:32,470 --> 00:17:37,350
+والمagnification علاقة عكسيةبالنسبة للـ Field of
+
+209
+00:17:37,350 --> 00:17:41,550
+Effusion الـ Field of Effusion هي الـ Surface Area
+
+210
+00:17:41,550 --> 00:17:45,950
+of View هي المساحة اللي أنا بدي أشوفها بقولك As a
+
+211
+00:17:45,950 --> 00:17:49,690
+magnification increase the area of view decrease
+
+212
+00:17:49,690 --> 00:17:54,370
+كل ما زادت قوتي التكبير كل ما قلت مساحة المنطقة
+
+213
+00:17:54,370 --> 00:17:59,170
+اللي أنا بدي أشوفها وهذا أشي طبيعي في 4X لو أنا
+
+214
+00:17:59,170 --> 00:18:03,770
+جبت ورقة شجر وهي في مرسومة موجودة في slide سابقة
+
+215
+00:18:06,880 --> 00:18:11,080
+بنسبة لـ 4x لو أنا جبت ورقة شجر و روحت حطيتها على
+
+216
+00:18:11,080 --> 00:18:15,520
+4x هشوف كل ورقة الشجر هتكون المساحة اللي انا بدي
+
+217
+00:18:15,520 --> 00:18:19,900
+اشوفها كتير كبيرة لكن لو انا زودت ال magnification
+
+218
+00:18:19,900 --> 00:18:23,460
+روحت على 10x بالتالي المساحة اللي انا شوفتها راحت
+
+219
+00:18:23,460 --> 00:18:27,740
+هذه الأزاق و شوفت فقط جزءأصغر من اللي أنا شوفته
+
+220
+00:18:27,740 --> 00:18:32,940
+فيه 4X وكذلك فيه بالنسبة لـ 40X كل ما زودت قوة
+
+221
+00:18:32,940 --> 00:18:35,700
+التكبير كل ما زاد الـ Magnification كل ما كانت
+
+222
+00:18:35,700 --> 00:18:38,420
+المساحة اللي أنا بدي أشوفها أقل راحت المساحة high
+
+223
+00:18:38,420 --> 00:18:42,600
+وشوفت فقط مساحة أقل شوفت high المساحة ونفس الفكرة
+
+224
+00:18:42,600 --> 00:18:47,000
+بالنسبة للـ Oil immersion هشوف فقط مساحة أقل هشوف
+
+225
+00:18:47,000 --> 00:18:51,420
+high المساحة فقط يبقى كانت علاقة عكسية بين الـ
+
+226
+00:18:51,420 --> 00:18:54,920
+Field of Fusion وما بين الـ Magnification
+
+227
+00:19:05,020 --> 00:19:08,720
+as a magnification increase the resolution also
+
+228
+00:19:08,720 --> 00:19:11,980
+increase اتفقنا كل ما زاد ال magnification كل ما
+
+229
+00:19:11,980 --> 00:19:14,940
+زاد ال resolution يعني اقدرت اميز بين نقطتين
+
+230
+00:19:14,940 --> 00:19:18,580
+المسافة بينهم كتير قليلة و لو بدنا نوضح اكتر هذه
+
+231
+00:19:18,580 --> 00:19:21,300
+النقطة لو انت كنت راكبة بسيارة و السيارة بتمشي
+
+232
+00:19:21,300 --> 00:19:25,880
+بسرعة معينة كان في ضوء أحمر من مسافة بعيدة كل ما
+
+233
+00:19:25,880 --> 00:19:29,620
+اقتربتي و كل ما اقدرتي ان اتميزي ان ضوء هذا عبارة
+
+234
+00:19:29,620 --> 00:19:35,240
+كان عن نصباحين فوق بعضوبالتالي قدرتي اتميزي هدول
+
+235
+00:19:35,240 --> 00:19:39,180
+الكائنين اللي كانت المسافة بينهم كتير قريبة انت كل
+
+236
+00:19:39,180 --> 00:19:43,040
+ما قربتي معناه بما يقابله في الميكروسكوب كل ما
+
+237
+00:19:43,040 --> 00:19:46,140
+كبرتوا كل ما زادت الـ Magnification كل ما قدرتي
+
+238
+00:19:46,140 --> 00:19:50,260
+انك اتميزي بين جسمان كائنين المسافة بينهم كتير
+
+239
+00:19:50,260 --> 00:19:53,840
+صغيرة to achieve a high magnification with good
+
+240
+00:19:53,840 --> 00:19:57,360
+resolution the objective lens must have a small
+
+241
+00:19:57,360 --> 00:20:02,250
+radius كلما وقولنا انه الـ numerical apertureالها
+
+242
+00:20:02,250 --> 00:20:06,510
+علاقة تعتمد على الـ «Radius of Length» وأنا قلت
+
+243
+00:20:06,510 --> 00:20:10,170
+إنه العلاقة عكسية نرجع شوية نرجع المعلومات كل ما
+
+244
+00:20:10,170 --> 00:20:13,090
+زاد الـ Magnification كل ما احتجت ميضه أكتر يعني
+
+245
+00:20:13,090 --> 00:20:16,490
+الـ numerical aperture كان عالي كل ما كان الـ
+
+246
+00:20:16,490 --> 00:20:19,750
+Resolution عالي طب أنا قلت ال numerical aperture
+
+247
+00:20:19,750 --> 00:20:23,810
+علاقته عكسية مع الـ Radius يبقى هيكون الـ Radius
+
+248
+00:20:23,810 --> 00:20:26,970
+علاقته عكسية مع كل من الـ Magnification ومع الـ
+
+249
+00:20:26,970 --> 00:20:30,430
+Resolutionيبقى وهذا اللي بيأكد هذا الكلام يبقى
+
+250
+00:20:30,430 --> 00:20:33,890
+Magnification هي لأ كل ما زاد لإن المرق الأبراطور
+
+251
+00:20:33,890 --> 00:20:37,390
+بيزيد ال Resolution بيزيد ويقل ال Radius بقالت
+
+252
+00:20:37,390 --> 00:20:42,450
+يبقى عدسة عشرة X ال Radius تبقى هيبقى أصغر من عدسة
+
+253
+00:20:42,450 --> 00:20:45,530
+أربعة X من هاي اللي اللي علاقة نستنفتش هذه
+
+254
+00:20:45,530 --> 00:20:50,410
+المعلومة shorter wavelength of light provide
+
+255
+00:20:50,410 --> 00:20:54,320
+greater resolutionاتفقنا حسب العلاقة اللي أخدناها
+
+256
+00:20:54,320 --> 00:20:57,000
+الـ «R» اللي هي انه قياس أصغر مسافة بين نقطتين
+
+257
+00:20:57,000 --> 00:21:01,920
+بتساوي 0.612 درب الـ «Wavelength» على الـ
+
+258
+00:21:01,920 --> 00:21:05,220
+«Numerical Aperture» إنه في عندي علاقة عكسية ما
+
+259
+00:21:05,220 --> 00:21:08,520
+بين الـ «Resolution» وما بين الـ «Wavelength» ما
+
+260
+00:21:08,520 --> 00:21:12,300
+بين الطول الموجي الطول الموجي هو .. الطول الموجي
+
+261
+00:21:12,300 --> 00:21:14,700
+هي خاصية في الضوء حسب شو أنا .. الضوء اللي أنا
+
+262
+00:21:14,700 --> 00:21:18,540
+استخدمته بستخدم الطول الموجي لهذا الضوء واللي
+
+263
+00:21:18,540 --> 00:21:22,930
+بيأكد هذا الكلام أنهالمشهر الإلكتروني أنا بستخدم
+
+264
+00:21:22,930 --> 00:21:27,370
+إلكترونات الطول الموجي للإلكترونات هي أقل من الطول
+
+265
+00:21:27,370 --> 00:21:30,970
+الموجي للضوء المريء عشان هيك ال resolution للمشهر
+
+266
+00:21:30,970 --> 00:21:34,890
+الإلكتروني بيكون أعلى من ال resolution للمشهر
+
+267
+00:21:34,890 --> 00:21:40,090
+الضوءي لأن ال wavelength للإلكترونات أقل من ال
+
+268
+00:21:40,090 --> 00:21:42,010
+wavelength للضوء المريء
+
+269
+00:21:45,220 --> 00:21:48,580
+هنعملّي شوية مراجعة على كل الإلاقات اللي اتكلمنا
+
+270
+00:21:48,580 --> 00:21:51,840
+عنها «depth of focus» الـ «depth of focus» قولنا
+
+271
+00:21:51,840 --> 00:21:56,240
+هو thickness of specimen هو ثمك المجال that can be
+
+272
+00:21:56,240 --> 00:22:00,120
+seen in focus at one time قولنا كل إلاقة عكسية ما
+
+273
+00:22:00,120 --> 00:22:02,520
+بين الـ magnification وما بين الـ depth of focus
+
+274
+00:22:02,520 --> 00:22:05,900
+الـ field of vision هو المساحة اللي أنا بدي أشوفها
+
+275
+00:22:05,900 --> 00:22:09,260
+قولنا إنه الإلاقة برضه عكسية يبقى الـ
+
+276
+00:22:09,260 --> 00:22:11,760
+magnification معقول من الـ depth of focus والـ
+
+277
+00:22:11,760 --> 00:22:15,610
+field of vision إلاقة عكسيةلكن الـ Magnification
+
+278
+00:22:15,610 --> 00:22:19,750
+مع الـ Resolution ومع الـ numerical aperture كلهم
+
+279
+00:22:19,750 --> 00:22:22,870
+علاقة ترضية كل ما زاد الـ Magnification كل ما
+
+280
+00:22:22,870 --> 00:22:25,730
+احتجت كمية الضوء اكتر تصل العينة بتتالي الـ
+
+281
+00:22:25,730 --> 00:22:29,050
+Resolution لزيها هاي ملخص كل العلاقات اللي اتكلمنا
+
+282
+00:22:29,050 --> 00:22:33,290
+فيها الـ Medium between the objective and the
+
+283
+00:22:33,290 --> 00:22:36,370
+object is a factor that must be taken into
+
+284
+00:22:36,370 --> 00:22:39,310
+consideration for the most effective use of
+
+285
+00:22:39,310 --> 00:22:44,110
+microscope lensبقولّك الوسط بين العدسة الشيئية وما
+
+286
+00:22:44,110 --> 00:22:48,410
+بين العينة لازم ناخدها بعين الاعتبار حتى إن هتكون
+
+287
+00:22:48,410 --> 00:22:52,030
+الصورة واضحة أنا قولت كل ما زاد الـ Magnification
+
+288
+00:22:52,030 --> 00:22:56,750
+كل ما زاد الـ Resolution لكن احنا قلنا لازم بعض
+
+289
+00:22:56,750 --> 00:23:00,930
+الأحيان أنه ناخد بعين الاعتبار أمور منها الـ Fine
+
+290
+00:23:00,930 --> 00:23:05,570
+Adjustment لما انا أروح من 4X أروح لـ40X لازم ألعب
+
+291
+00:23:05,570 --> 00:23:09,370
+بالـFine Adjustment لازم أتحكم بإداء فرق من كمية
+
+292
+00:23:09,370 --> 00:23:12,890
+الضوءواصلة لهذه العينة من الأمور المهمة اللي لازم
+
+293
+00:23:12,890 --> 00:23:18,130
+أخدها بعين الاعتبار منها برضه الوسط ما بين العدسة
+
+294
+00:23:18,130 --> 00:23:22,030
+الشيئية وما بين الـ Slots بقولك The Lower Power
+
+295
+00:23:22,030 --> 00:23:27,150
+Objective 4X 10X and High Dry Objective 40X Loose
+
+296
+00:23:27,150 --> 00:23:31,910
+Air احنا قلنا ان الـ 4X اسمها Scanning Objective
+
+297
+00:23:31,910 --> 00:23:36,560
+لكن هنا هو مسميها Low Powerلكن إحنا خلّينا نتفق
+
+298
+00:23:36,560 --> 00:23:41,080
+إنه الـ 4X هي تعتبر Scanning الـ 10X تعتبر Law
+
+299
+00:23:41,080 --> 00:23:45,000
+Power لكن لو أنا سميت الـ 4X Law Power عادي هي
+
+300
+00:23:45,000 --> 00:23:48,680
+Scanning أو Law Power ولكن الـ High Power هي الـ
+
+301
+00:23:48,680 --> 00:23:53,180
+40X أقولك في العدسة الشيئية 4 وفي العدسة الشيئية
+
+302
+00:23:53,180 --> 00:23:57,700
+10X و40X باستخدام يكون الوسط ما بين العدسة الشيئية
+
+303
+00:23:57,700 --> 00:24:02,020
+وما بين الـ Slide يكون الهواء لكن عند العدسة 100
+
+304
+00:24:02,930 --> 00:24:05,930
+الـ Oil Emergent بستخدم الـ Oil عشان هي احنا
+
+305
+00:24:05,930 --> 00:24:10,090
+بنسميها Oil Emergent لأنه بنضيف الـ Oil طيب ليش
+
+306
+00:24:10,090 --> 00:24:15,410
+أنا بضيف Oil عند 100X؟ بقولك لأن الـ Z يلو نفس
+
+307
+00:24:15,410 --> 00:24:20,690
+معامل الزجاج حلو لما ينتقل الضوء مابان وسطين معامل
+
+308
+00:24:20,690 --> 00:24:24,870
+انكسار هو مختلف بتشطط الضوء بتشطط الضوء يعني ال
+
+309
+00:24:24,870 --> 00:24:28,830
+numerical aperture يعني كمية الضوء الواصلة للعينة
+
+310
+00:24:28,830 --> 00:24:32,900
+بتكون قليلةبالتالي الـ Resolution هيقل وضوح الصورة
+
+311
+00:24:32,900 --> 00:24:36,640
+هيقل، عشان هيك أنا بستخدم الـ Oil اللي له نفس
+
+312
+00:24:36,640 --> 00:24:41,200
+معامل الزجاج، وبالتالي مش هيتشطط الضوء، هيتجمع على
+
+313
+00:24:41,200 --> 00:24:45,640
+العدسة، هيوصل كمية كبيرة الضوء لعينة، numerical
+
+314
+00:24:45,640 --> 00:24:48,700
+aperture هيكون عالي، بالتالي هيكون الـ Resolution
+
+315
+00:24:48,700 --> 00:24:54,500
+وضوح الصورة أكتر في حالة أن أنا لو ضفت Oilتمام،
+
+316
+00:24:54,500 --> 00:24:57,540
+هذا بالنسبة للسبب إن أنا بحط Oil ‏when oil
+
+317
+00:24:57,540 --> 00:25:00,400
+emergence lens are employed ‏a drop of oil should
+
+318
+00:25:00,400 --> 00:25:02,820
+be used ‏أقول لك، لو أنا استخدمت الـ Oil emergence
+
+319
+00:25:02,820 --> 00:25:07,780
+lens ‏اللي احنا سميناها هي الـ 100X Objective
+
+320
+00:25:07,780 --> 00:25:12,740
+‏بقولك، نضيف نقطة من إزاي ‏وليه هذا السبب؟
+
+321
+00:25:12,740 --> 00:25:15,520
+‏binding of the light waves and cursants ‏لأن
+
+322
+00:25:15,520 --> 00:25:20,830
+بيصير في عندي انحناء للضوءOil has the same
+
+323
+00:25:20,830 --> 00:25:24,310
+refractive index of the glass while air in the
+
+324
+00:25:24,310 --> 00:25:28,030
+case of refraction لأن معامل انكسار ذات إنه نفس
+
+325
+00:25:28,030 --> 00:25:32,330
+معامل انكسار الزرجاج لكن بالنسبة للهواء معامل
+
+326
+00:25:32,330 --> 00:25:37,190
+انكسار الهواء أقل من الزرجاج وهذا بيقدّي إنه
+
+327
+00:25:37,190 --> 00:25:41,690
+يتشدّر الضوء أكتر أتقل كمية الضوء الواصلة للعينة
+
+328
+00:25:41,690 --> 00:25:43,450
+بالتالي الـ Resolution تقل
+
+329
+00:25:47,540 --> 00:25:52,600
+هنحملك صورة مقارنة بين لو أنا بدون ما أحط oil على
+
+330
+00:25:52,600 --> 00:25:57,580
+100X و لو أنا حطيت oil على العدسة 100 لو أنا ما
+
+331
+00:25:57,580 --> 00:26:01,920
+حطيت oil على العدسة 100 بقولك إنه لما يمر الضوء،
+
+332
+00:26:01,920 --> 00:26:07,100
+الضوء هيتشتت فبالتالي مش هيوصل كمية كبيرة للعينة
+
+333
+00:26:07,100 --> 00:26:11,700
+مش هيوصل كمية كبيرة من الضوء للعينة أو للعدسة
+
+334
+00:26:11,700 --> 00:26:15,300
+الشيئية فبالتالي الـ Resolution ألف لكن لو أنا
+
+335
+00:26:15,300 --> 00:26:19,380
+استخدمت الـ Oilبالتالي الضوء مش هيتشتت هيتجمع على
+
+336
+00:26:19,380 --> 00:26:24,000
+العدس الشيئية هيوصل كمية الضوء كبيرة فبالتالي ال
+
+337
+00:26:24,000 --> 00:26:29,140
+resolution وضوح الصورة بزيه Refractive إيش يعني؟
+
+338
+00:26:29,140 --> 00:26:32,420
+Refractive يعني bending إنكسار أو إنحناء
+
+339
+00:26:32,420 --> 00:26:35,240
+Refractive index is a measurement of the extent
+
+340
+00:26:35,240 --> 00:26:39,440
+that a substance bends light هو بيقولك بيقيس معدل
+
+341
+00:26:39,440 --> 00:26:45,220
+إنكسار الضوءfield of view or equal لو كان اللي هو
+
+342
+00:26:45,220 --> 00:26:48,060
+الـ total magnification field of view يعني الـ
+
+343
+00:26:48,060 --> 00:26:51,140
+total magnification بقولك لو كان أكبر أو يساوي
+
+344
+00:26:51,140 --> 00:26:56,000
+500x cause increase in diffraction and this cause
+
+345
+00:26:56,000 --> 00:26:59,300
+distortion of the image مش معناه، هلأ أنا لما
+
+346
+00:26:59,300 --> 00:27:02,960
+أستخدم 4x وقولنا معامل الإنكسار أو total sorry
+
+347
+00:27:02,960 --> 00:27:07,700
+total magnification بكون 40 لو أنا استخدمت 10x
+
+348
+00:27:07,700 --> 00:27:12,710
+بكون اللي هي100 Total Magnification لو أنا استخدمت
+
+349
+00:27:12,710 --> 00:27:18,210
+40X الـ Total Magnification بيكون 400X طيب لو أنا
+
+350
+00:27:18,210 --> 00:27:22,050
+استخدمت 100 بيكون الـ Total Magnification 1000X
+
+351
+00:27:22,050 --> 00:27:26,190
+وين الـ Total Magnification بيكون أعلى من 500 لو
+
+352
+00:27:26,190 --> 00:27:30,130
+أنا استخدمت اللي هي الـ 100X Objective لو أنا
+
+353
+00:27:30,130 --> 00:27:33,750
+استخدمت عدسة العدسة الشيئية 100 هيكون الـ Total
+
+354
+00:27:33,750 --> 00:27:38,440
+Magnification أكتر من 500فبالتالي في العدسة
+
+355
+00:27:38,440 --> 00:27:43,060
+الشيئية 100X بيقولّك بيزيد لما يكون الـ Total
+
+356
+00:27:43,060 --> 00:27:46,640
+Magnification أكتر من 500 اللي هو عن 100X بيزيد
+
+357
+00:27:46,640 --> 00:27:51,360
+تشطط الضوء فبالتالي عشان هيك أنا بوضع .. بحط oil
+
+358
+00:27:51,360 --> 00:27:54,180
+عند الـ 100X لأن الـ Total Magnification اللي
+
+359
+00:27:54,180 --> 00:27:58,340
+نبعها أكتر من 500 بالتالي هيكون عند الـ numerical
+
+360
+00:27:58,340 --> 00:28:01,340
+aperture هيتشطط الضوء ال numerical aperture هيكون
+
+361
+00:28:01,340 --> 00:28:05,490
+قليل ال-resolution قليل بالتاليقلتها تسبب
+
+362
+00:28:05,490 --> 00:28:08,430
+Distortion of the image ‏هيكون فيها اندى قلة في
+
+363
+00:28:08,430 --> 00:28:11,710
+وضوح الصورة ‏وقلنا ليش لأنه اتشتت اللوء ما تجمع
+
+364
+00:28:11,710 --> 00:28:15,350
+على العينة ‏Caline numerical aperture ألي ‏هذا
+
+365
+00:28:15,350 --> 00:28:19,210
+بالنسبة للعلاقات
+
+366
+00:28:19,210 --> 00:28:23,410
+المهمة ‏بالنسبة لل light compound microscopeهلأ
+
+367
+00:28:23,410 --> 00:28:26,650
+بقولك كيف أنا أستخدم الـMicroscope أول شيء طبعا
+
+368
+00:28:26,650 --> 00:28:32,570
+بحط الفيشة في الكهربا بضوي مصدر الإضاءة و بحط على
+
+369
+00:28:32,570 --> 00:28:37,490
+أربعة ببدأ أنا بالـLow Power و بعدين بنتقل لـHigh
+
+370
+00:28:37,490 --> 00:28:42,190
+Power بحط على 4X Objective بحط الـSmear على
+
+371
+00:28:42,190 --> 00:28:44,590
+الـStage الـSmear يعني أني أنا بحط الـSlide أو
+
+372
+00:28:44,590 --> 00:28:48,030
+العين اللي أنا بديها على الـStage و بشوفها على
+
+373
+00:28:48,030 --> 00:28:52,870
+الـ4X بستخدم الـCourse Adjustment Logأحنا قلنا إن
+
+374
+00:28:52,870 --> 00:28:55,970
+ال course adjustment knob حتى إن أنا أستخدمه حتى
+
+375
+00:28:55,970 --> 00:29:01,170
+إن أنا أجيب ال fail فبحركه طبعا ممكن إن أنا أحط ال
+
+376
+00:29:01,170 --> 00:29:06,010
+stage أخليها للآخر اللي فوق و أبدأ إن أنا أنزل أو
+
+377
+00:29:06,010 --> 00:29:09,530
+إن أنا بنزلها للآخر و ببدأ بتطلع شوية شوية حتى إن
+
+378
+00:29:09,530 --> 00:29:12,630
+أنا أشوف الصورة اللي أنا بديها هيك معناه إن أنا
+
+379
+00:29:12,630 --> 00:29:16,650
+جبت ال failبقولك بعدين بتنتقلي للـ «objective
+
+380
+00:29:16,650 --> 00:29:20,690
+lens» التانية العشرة X وما بعدها اللي هي الأربعين
+
+381
+00:29:20,690 --> 00:29:25,530
+وأخر شئ بتوصلي طبعا لما توصلي لأربعين بتحاول أنك
+
+382
+00:29:25,530 --> 00:29:28,650
+تحركي الـFine Adjustment حتى أنه تكون
+
+383
+00:29:28,650 --> 00:29:32,510
+الـResolution للـImage واضحة لما تستخدمي الـOil
+
+384
+00:29:32,510 --> 00:29:35,930
+Immersion بقولك بتحطي Drop of Oil واتفقنا ليش
+
+385
+00:29:35,930 --> 00:29:39,890
+بإننا بنحط Oil على الـ100X كافي بتمسكي
+
+386
+00:29:39,890 --> 00:29:43,740
+الـMicroscope بقولك بتمسكيهو إيد بتمسك الـ ARM
+
+387
+00:29:43,740 --> 00:29:46,760
+وإيد تانية بتمسك الـ Bezel زي ما إحنا شايفين أنه
+
+388
+00:29:46,760 --> 00:29:50,440
+هان إيد ماسكة الـ Bezel قاعدة والإيد التانية ماسكة
+
+389
+00:29:50,440 --> 00:29:55,360
+الـ ARM في بعض العوامل اللي بتأثر على جودة الصورة
+
+390
+00:29:55,360 --> 00:29:58,860
+Quality of Microscope الـ Antlens اتكلمنا عن هذا
+
+391
+00:29:58,860 --> 00:30:03,460
+الموضوع أنه جودة الـ Microscope و العدسات طبعا
+
+392
+00:30:03,460 --> 00:30:07,700
+مثلا على سبيل المثال الـ Microscope الإلكتروني الـ
+
+393
+00:30:07,700 --> 00:30:10,560
+Resolution تبعه أكيد هيكون أعلى من الـ Microscope
+
+394
+00:30:10,560 --> 00:30:13,720
+الضوءيالـ Size of sample، الـ type of sample أو
+
+395
+00:30:13,720 --> 00:30:16,700
+mouth of light on the sample كمية الضوء الواصلة
+
+396
+00:30:16,700 --> 00:30:22,240
+للعينة وجودة العينة كل هذه الأمور بتأثر على جودة
+
+397
+00:30:22,240 --> 00:30:26,020
+الصورة Microscope Maintenance كيف أنا بدي أحافظ
+
+398
+00:30:26,020 --> 00:30:31,420
+على الـ Microscope بقولك ضروري أني أنا أحافظ أني
+
+399
+00:30:31,420 --> 00:30:34,680
+أنا أخزنه بالطريقة الصحية هاي هتمنع كتير من
+
+400
+00:30:34,680 --> 00:30:38,820
+المشاكلهحميه من الـ Dust and dirt بدي أحميه من
+
+401
+00:30:38,820 --> 00:30:42,360
+الغبار و من الفندس و من الفطريات ا start the
+
+402
+00:30:42,360 --> 00:30:44,880
+microscope under a protection cover في عندي
+
+403
+00:30:44,880 --> 00:30:47,780
+protection cover كيس خاص فيه ال microscope بعد ما
+
+404
+00:30:47,780 --> 00:30:51,880
+أخلص و أنظف ال microscope بحطه فيه ال protection
+
+405
+00:30:51,880 --> 00:30:57,500
+cover و بحطه في ظروف تكون الرطوبة فيها قليلة كيف
+
+406
+00:30:57,500 --> 00:31:01,080
+أنا بدي أنظف ال microscope بقولك بستخدم لأ ال body
+
+407
+00:31:01,080 --> 00:31:05,820
+و للا ال stage اللي هي صبونةأما بالنسبة لأ العدسات
+
+408
+00:31:05,820 --> 00:31:08,060
+حتى أني أنا مضت في العدسات باستخدام الـ Ether
+
+409
+00:31:08,060 --> 00:31:11,280
+Alcohol أو الـ Alcohol أو في عندي Lens Cleaner
+
+410
+00:31:11,280 --> 00:31:17,960
+Solution Cleaner سائل خاص بالعدسات للتنظيف بالنسبة
+
+411
+00:31:17,960 --> 00:31:23,960
+لأدوات الـ Lens Paper بقولك في عندي بعض الـ Lens
+
+412
+00:31:23,960 --> 00:31:27,060
+Paper اللي هو Commercial Lens Tissue For Optics
+
+413
+00:31:27,060 --> 00:31:32,750
+بتكون ورق تجاري خاص بتنظيف العدسات بقولكاحذري أنك
+
+414
+00:31:32,750 --> 00:31:36,310
+انت تستخدمي rough paper protect انه يكون اللي هو
+
+415
+00:31:36,310 --> 00:31:40,710
+الورق يكون خشن لأنه هيك هيأذي العدسات ممكن بعض
+
+416
+00:31:40,710 --> 00:31:45,170
+الأمور التانية اللي انك تستبدليها ب fine tissue
+
+417
+00:31:45,170 --> 00:31:49,670
+paper اللي هو الكهنس الناعم مثلا ال cloth اللي هو
+
+418
+00:31:49,670 --> 00:31:53,470
+الشاس سلك اللي هو الحريق ممكن تستخدمي هدول كلهم في
+
+419
+00:31:53,470 --> 00:31:57,910
+تنظيف العدساتكيف انا بدي انظف من الـ IBs الـ
+
+420
+00:31:57,910 --> 00:32:01,810
+Objective الـ Microscope Stage الـ Microscope Body
+
+421
+00:32:01,810 --> 00:32:05,970
+الـ Condenser بقولك بالنسبة للـ IBs انت بتنظفيهم
+
+422
+00:32:05,970 --> 00:32:09,970
+بحركة دائرية Circular مواشن من الداخل للخارج او
+
+423
+00:32:09,970 --> 00:32:15,850
+زيجزاجي وبعدين طبعا بتنشفيهم بالنسبة لل IBs هي بعد
+
+424
+00:32:15,850 --> 00:32:19,430
+ما انت اتخلص بتعملي ال drying اذا انت احتجتي مرة
+
+425
+00:32:19,430 --> 00:32:23,820
+اخرى لتنظيف مطفيها و تعملي ال drying كمان اخرىالـ
+
+426
+00:32:23,820 --> 00:32:27,140
+Objective lens ممنوع انك انت تشيليها من الـ
+
+427
+00:32:27,140 --> 00:32:29,420
+Nosepiece الـ Nosepiece تكلمنا عنه القرص اللي هو
+
+428
+00:32:29,420 --> 00:32:33,520
+اللي بيحرك العدسات ممنوع تشيلي العدسات الشيئية من
+
+429
+00:32:33,520 --> 00:32:38,140
+الـ Nosepiece اخر شئ لو انت بدك تنطفي ال body
+
+430
+00:32:38,140 --> 00:32:42,100
+بقولك لازم ترفع الفيشة من الكهربا و بعدين تنطفي ال
+
+431
+00:32:42,100 --> 00:32:48,510
+body حق بيقولنا بصابونةحتى إنك تزيل كل القادورات
+
+432
+00:32:48,510 --> 00:32:51,950
+عنها أخر الشيء الـ Condenser أكيد لما قبل ما تنطفي
+
+433
+00:32:51,950 --> 00:32:55,530
+بتدخل فيه شغلناه بتنطفي الـ Condenser بـ Lentifree
+
+434
+00:32:55,530 --> 00:32:59,710
+Cotton الـ Swab و بتستخدمي الـ Lens Cleaning
+
+435
+00:32:59,710 --> 00:33:05,690
+Solution و أخر الشيء بتعملي .. بتمسحيه بـ Dry Swab
+
+436
+00:33:05,690 --> 00:33:10,030
+فهيك بنكون خلصنا معمل الـ Microscope ان شاء الله
+
+437
+00:33:10,030 --> 00:33:14,040
+تكون كل المعلومات واضحة بالنسبة لكمهيكون فينا
+
+438
+00:33:14,040 --> 00:33:18,320
+مناقشة الأسبوع اللي جاي يوم التلاتة الساعة أحد عشر
+
+439
+00:33:18,320 --> 00:33:22,500
+هيكون تكون المناقشة في معمل الـ Microscope ومعمل
+
+440
+00:33:22,500 --> 00:33:25,840
+الـ Introduction إذا في أي معلومات إن شاء الله
+
+441
+00:33:25,840 --> 00:33:29,800
+هنقشها في هذا اللقاء السلام عليكم ورحمة الله
+
+442
+00:33:29,800 --> 00:33:30,440
+وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/a4LrdmbI9CQ_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/a4LrdmbI9CQ_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..ed1f75353b12a9f97a9bbb5e9a4d359f650a4525
--- /dev/null
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+{"segments": [{"id": 1, "seek": 3005, "start": 0.37, "end": 30.05, "text": "في هاي ال slide هنتكلم عن بعض النقاط المهمة بالنسبة لل light compound microscope أول شئ ال magnification ال magnification هي قوة التكبير relative enlargement of the specimen هو قوة تكبير العين أو الشريحة حتى أحصل على ال total magnification بضرب ال Ibs magnification ضرب ال objective magnification قوة التكبير العدسة العينية اللي هي الocular lens magnification اللي هي ثابت 10X", "tokens": [41185, 8032, 47302, 2423, 4137, 8032, 29399, 4117, 19528, 18871, 45030, 11242, 28239, 4587, 41193, 9673, 16095, 3660, 20666, 1863, 35457, 3660, 24976, 1442, 14154, 29753, 5551, 12610, 13412, 19986, 2423, 4944, 3774, 2423, 4944, 3774, 39896, 12174, 2407, 3660, 16712, 4117, 3555, 13546, 4972, 31976, 48122, 295, 264, 34204, 31439, 12174, 2407, 3660, 6055, 4117, 3555, 13546, 18863, 9957, 34051, 25124, 16572, 5016, 3660, 11331, 49975, 5551, 5016, 36520, 15844, 2423, 3217, 4944, 3774, 4724, 11242, 25513, 2423, 286, 929, 4944, 3774, 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270.68, "word": " ده", "probability": 0.4871826171875}, {"start": 270.68, "end": 271.32, "word": " هسميها", "probability": 0.882568359375}, {"start": 271.32, "end": 271.5, "word": " الـ", "probability": 0.819580078125}, {"start": 271.5, "end": 271.98, "word": " Scanning", "probability": 0.773193359375}, {"start": 271.98, "end": 272.22, "word": " Power", "probability": 0.68359375}, {"start": 272.22, "end": 272.54, "word": " هيك", "probability": 0.522857666015625}, {"start": 272.54, "end": 272.72, "word": " أنا", "probability": 0.90673828125}, {"start": 272.72, "end": 273.22, "word": " حصلت", "probability": 0.9899088541666666}, {"start": 273.22, "end": 273.44, "word": " على", "probability": 0.8701171875}, {"start": 273.44, "end": 273.72, "word": " الـ", "probability": 0.9453125}, {"start": 273.72, "end": 273.94, "word": " Total", "probability": 0.9169921875}, {"start": 273.94, "end": 274.8, "word": " Magnification", "probability": 0.944091796875}, {"start": 274.8, "end": 280.18, "word": " خلصنا", "probability": 0.91796875}, {"start": 280.18, "end": 280.38, "word": " من", "probability": 0.9970703125}, {"start": 280.38, "end": 280.64, "word": " الـ", "probability": 0.908447265625}, {"start": 280.64, "end": 281.3, "word": " Magnification", "probability": 0.897705078125}, {"start": 281.3, "end": 281.66, "word": " ومن", "probability": 0.792724609375}, {"start": 281.66, "end": 281.96, "word": " الـ", "probability": 0.980712890625}, {"start": 281.96, "end": 282.16, "word": " Total", "probability": 0.91162109375}, {"start": 282.16, "end": 283.14, "word": " Magnification", "probability": 0.945556640625}, {"start": 283.14, "end": 283.82, "word": " في", "probability": 0.81787109375}, {"start": 283.82, "end": 284.16, "word": " أنا", "probability": 0.810546875}, {"start": 284.16, "end": 284.56, "word": " عندي", "probability": 0.79052734375}, {"start": 284.56, "end": 285.12, "word": " Slide", "probability": 0.58154296875}, {"start": 285.12, "end": 285.34, "word": " اللي", "probability": 0.955810546875}, {"start": 285.34, "end": 285.56, "word": " هي", "probability": 0.97314453125}, {"start": 285.56, "end": 285.74, "word": " الـ", "probability": 0.932373046875}, {"start": 285.74, "end": 286.28, "word": " Numerical", "probability": 0.7943522135416666}, {"start": 286.28, "end": 286.92, "word": " Aperture", "probability": 0.6449381510416666}, {"start": 286.92, "end": 287.18, "word": " لكن", "probability": 0.91015625}, {"start": 287.18, "end": 287.48, "word": " قبل", "probability": 0.99658203125}, {"start": 287.48, "end": 287.6, "word": " ما", "probability": 0.841796875}, {"start": 287.6, "end": 288.02, "word": " أتكلم", "probability": 0.9140625}, {"start": 288.02, "end": 288.3, "word": " على", "probability": 0.83642578125}, {"start": 288.3, "end": 288.58, "word": " الـ", "probability": 0.930908203125}, {"start": 288.58, "end": 289.02, "word": " Numerical", "probability": 0.9552408854166666}, {"start": 289.02, "end": 289.7, "word": " Aperture", "probability": 0.826171875}, {"start": 289.7, "end": 289.96, "word": " بدي", "probability": 0.698486328125}, {"start": 289.96, "end": 290.52, "word": " أتكلم", "probability": 0.9666748046875}, {"start": 290.52, "end": 290.92, "word": " عن", "probability": 0.9697265625}, {"start": 290.92, "end": 291.48, "word": " الـ", "probability": 0.8720703125}, {"start": 291.48, "end": 292.1, "word": " Resolution", "probability": 0.95068359375}, {"start": 292.1, "end": 293.24, "word": " كثير", "probability": 0.8377278645833334}, {"start": 293.24, "end": 293.86, "word": " حكينا", "probability": 0.9942626953125}, {"start": 293.86, "end": 294.18, "word": " في", "probability": 0.99462890625}, {"start": 294.18, "end": 294.54, "word": " الـ", "probability": 0.899658203125}, {"start": 294.54, "end": 295.1, "word": " Resolution", "probability": 0.955810546875}, {"start": 295.1, "end": 295.46, "word": " لكن", "probability": 0.935546875}, {"start": 295.46, "end": 295.9, "word": " بدنا", "probability": 0.893310546875}, {"start": 295.9, "end": 296.7, "word": " نعرفه", "probability": 0.833740234375}, {"start": 296.7, "end": 297.6, "word": " بشكل", "probability": 0.9778645833333334}, {"start": 297.6, "end": 298.0, "word": " علمي", "probability": 0.8644205729166666}], "temperature": 1.0}, {"id": 12, "seek": 32591, "start": 298.35, "end": 325.91, "text": "الـ Resolution it is the ability of microscope to distinguish two points يقولك هو قدرة ال microscope أو العدسة أنه يميز بين نقطتين نكمل التعريف أن هدول النقطتين يكونوا المسافة بينهم قليلة يبقى that how small distance between it نحكي that how small", "tokens": [6027, 39184, 5015, 3386, 309, 307, 264, 3485, 295, 29753, 281, 20206, 732, 2793, 7251, 39648, 4117, 31439, 12174, 3215, 25720, 2423, 4532, 4417, 1114, 34051, 18863, 3215, 3794, 3660, 14739, 3224, 7251, 2304, 1829, 11622, 49374, 8717, 47432, 2655, 9957, 8717, 24793, 1211, 16712, 3615, 16572, 5172, 14739, 8032, 3215, 12610, 28239, 47432, 2655, 9957, 7251, 30544, 14407, 9673, 3794, 31845, 3660, 49374, 16095, 12174, 20292, 37977, 7251, 3555, 4587, 7578, 300, 577, 1359, 4560, 1296, 309, 8717, 5016, 4117, 1829, 300, 577, 1359], "avg_logprob": -0.19349563260411107, "compression_ratio": 1.5176991150442478, "no_speech_prob": 9.5367431640625e-07, "words": [{"start": 298.35, "end": 298.61, "word": "الـ", "probability": 0.8310546875}, {"start": 298.61, "end": 299.19, "word": " Resolution", "probability": 0.749267578125}, {"start": 299.19, "end": 299.57, "word": " it", "probability": 0.317626953125}, {"start": 299.57, "end": 299.85, "word": " is", "probability": 0.9521484375}, {"start": 299.85, "end": 300.19, "word": " the", "probability": 0.72998046875}, {"start": 300.19, "end": 300.79, "word": " ability", "probability": 0.94921875}, {"start": 300.79, "end": 301.15, "word": " of", "probability": 0.95361328125}, {"start": 301.15, "end": 301.87, "word": " microscope", "probability": 0.71142578125}, {"start": 301.87, "end": 302.45, "word": " to", "probability": 0.91748046875}, {"start": 302.45, "end": 303.35, "word": " distinguish", "probability": 0.9501953125}, {"start": 303.35, "end": 303.79, "word": " two", "probability": 0.8876953125}, {"start": 303.79, "end": 304.15, "word": " points", "probability": 0.90380859375}, {"start": 304.15, "end": 304.59, "word": " يقولك", "probability": 0.5985107421875}, {"start": 304.59, "end": 304.73, "word": " هو", "probability": 0.7900390625}, {"start": 304.73, "end": 305.25, "word": " قدرة", "probability": 0.9752604166666666}, {"start": 305.25, "end": 305.49, "word": " ال", "probability": 0.740234375}, {"start": 305.49, "end": 306.37, "word": " microscope", "probability": 0.7172037760416666}, {"start": 306.37, "end": 306.69, "word": " أو", "probability": 0.8876953125}, {"start": 306.69, "end": 307.51, "word": " العدسة", "probability": 0.9766845703125}, {"start": 307.51, "end": 308.29, "word": " أنه", "probability": 0.74267578125}, {"start": 308.29, "end": 308.85, "word": " يميز", "probability": 0.9774169921875}, {"start": 308.85, "end": 309.07, "word": " بين", "probability": 0.98193359375}, {"start": 309.07, "end": 309.69, "word": " نقطتين", "probability": 0.973388671875}, {"start": 309.69, "end": 310.73, "word": " نكمل", "probability": 0.72509765625}, {"start": 310.73, "end": 311.41, "word": " التعريف", "probability": 0.9859619140625}, {"start": 311.41, "end": 311.67, "word": " أن", "probability": 0.475341796875}, {"start": 311.67, "end": 312.09, "word": " هدول", "probability": 0.8001302083333334}, {"start": 312.09, "end": 312.93, "word": " النقطتين", "probability": 0.86865234375}, {"start": 312.93, "end": 313.53, "word": " يكونوا", "probability": 0.694580078125}, {"start": 313.53, "end": 314.25, "word": " المسافة", "probability": 0.9771728515625}, {"start": 314.25, "end": 314.67, "word": " بينهم", "probability": 0.974853515625}, {"start": 314.67, "end": 315.59, "word": " قليلة", "probability": 0.769287109375}, {"start": 315.59, "end": 316.81, "word": " يبقى", "probability": 0.9532470703125}, {"start": 316.81, "end": 317.35, "word": " that", "probability": 0.48193359375}, {"start": 317.35, "end": 317.75, "word": " how", "probability": 0.8564453125}, {"start": 317.75, "end": 318.13, "word": " small", "probability": 0.9462890625}, {"start": 318.13, "end": 318.75, "word": " distance", "probability": 0.9013671875}, {"start": 318.75, "end": 319.27, "word": " between", "probability": 0.92138671875}, {"start": 319.27, "end": 319.95, "word": " it", "probability": 0.93701171875}, {"start": 319.95, "end": 321.05, "word": " نحكي", "probability": 0.9403076171875}, {"start": 321.05, "end": 321.41, "word": " that", "probability": 0.85498046875}, {"start": 321.41, "end": 323.47, "word": " how", "probability": 0.84423828125}, {"start": 323.47, "end": 325.91, "word": " small", "probability": 0.9482421875}], "temperature": 1.0}, {"id": 13, "seek": 34584, "start": 331.2, "end": 345.84, "text": "distance between them هو قدرة الميكروسكوب أو العدسة يميز بين نقطتين المسافة بينهم قليلة", "tokens": [67, 20829, 1296, 552, 31439, 12174, 3215, 25720, 9673, 1829, 4117, 32887, 3794, 4117, 37746, 34051, 18863, 3215, 3794, 3660, 7251, 2304, 1829, 11622, 49374, 8717, 47432, 2655, 9957, 9673, 3794, 31845, 3660, 49374, 16095, 12174, 20292, 37977], "avg_logprob": -0.1974158600354806, "compression_ratio": 1.2033898305084745, "no_speech_prob": 2.9981136322021484e-05, "words": [{"start": 331.20000000000005, "end": 332.6, "word": "distance", "probability": 0.5970458984375}, {"start": 332.6, "end": 334.0, "word": " between", "probability": 0.849609375}, {"start": 334.0, "end": 338.16, "word": " them", "probability": 0.873046875}, {"start": 338.16, "end": 340.1, "word": " هو", "probability": 0.44140625}, {"start": 340.1, "end": 340.46, "word": " قدرة", "probability": 0.9729817708333334}, {"start": 340.46, "end": 341.36, "word": " الميكروسكوب", "probability": 0.8103376116071429}, {"start": 341.36, "end": 341.6, "word": " أو", "probability": 0.8037109375}, {"start": 341.6, "end": 342.12, "word": " العدسة", "probability": 0.9066162109375}, {"start": 342.12, "end": 342.58, "word": " يميز", "probability": 0.9688720703125}, {"start": 342.58, "end": 342.82, "word": " بين", "probability": 0.97705078125}, {"start": 342.82, "end": 343.54, "word": " نقطتين", "probability": 0.9111328125}, {"start": 343.54, "end": 344.6, "word": " المسافة", "probability": 0.9429931640625}, {"start": 344.6, "end": 344.98, "word": " بينهم", "probability": 0.9716796875}, {"start": 344.98, "end": 345.84, "word": " قليلة", "probability": 0.6807861328125}], "temperature": 1.0}, {"id": 14, "seek": 37970, "start": 353.68, "end": 379.7, "text": "The smaller the distance between the two specific objectives that can be distinguished apart, the greater the resolution power of the microscope وقولّك كل ما كانت المسافة ما بين جثمان مختلفان وانا قدرت اميز بين هدول الجثمان كل ما كانت المسافة الصغيرة وانا قدرت اميز بين هدول الجثمان كل ما كان ال resolution power لهذا ال microscope عالي", "tokens": [2278, 4356, 264, 4560, 1296, 264, 732, 2685, 15961, 300, 393, 312, 21702, 4936, 11, 264, 5044, 264, 8669, 1347, 295, 264, 29753, 4032, 39648, 11703, 4117, 28242, 19446, 25961, 2655, 9673, 3794, 31845, 3660, 19446, 49374, 10874, 12984, 2304, 7649, 3714, 46456, 46538, 7649, 4032, 7649, 995, 12174, 3215, 43500, 1975, 2304, 1829, 11622, 49374, 8032, 3215, 12610, 25724, 12984, 2304, 7649, 28242, 19446, 25961, 2655, 9673, 3794, 31845, 3660, 31767, 17082, 48923, 4032, 7649, 995, 12174, 3215, 43500, 1975, 2304, 1829, 11622, 49374, 8032, 3215, 12610, 25724, 12984, 2304, 7649, 28242, 19446, 25961, 2423, 8669, 1347, 46740, 15730, 2423, 4532, 4417, 1114, 6225, 6027, 1829], "avg_logprob": -0.1533564778941649, "compression_ratio": 1.9297520661157024, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 353.68, "end": 353.9, "word": "The", "probability": 0.07269287109375}, {"start": 353.9, "end": 354.46, "word": " smaller", "probability": 0.849609375}, {"start": 354.46, "end": 354.74, "word": " the", "probability": 0.85693359375}, {"start": 354.74, "end": 355.24, "word": " distance", "probability": 0.955078125}, {"start": 355.24, "end": 355.76, "word": " between", "probability": 0.91455078125}, {"start": 355.76, "end": 356.12, "word": " the", "probability": 0.79052734375}, {"start": 356.12, "end": 356.48, "word": " two", "probability": 0.931640625}, {"start": 356.48, "end": 357.08, "word": " specific", "probability": 0.92333984375}, {"start": 357.08, "end": 357.88, "word": " objectives", "probability": 0.59228515625}, {"start": 357.88, "end": 358.36, "word": " that", "probability": 0.67529296875}, {"start": 358.36, "end": 358.78, "word": " can", "probability": 0.9609375}, {"start": 358.78, "end": 359.0, "word": " be", "probability": 0.9580078125}, {"start": 359.0, "end": 359.68, "word": " distinguished", "probability": 0.9013671875}, {"start": 359.68, "end": 360.32, "word": " apart,", "probability": 0.91259765625}, {"start": 360.78, "end": 360.94, "word": " the", "probability": 0.7861328125}, {"start": 360.94, "end": 361.44, "word": " greater", "probability": 0.93310546875}, {"start": 361.44, "end": 361.88, "word": " the", "probability": 0.91748046875}, {"start": 361.88, "end": 362.34, "word": " resolution", "probability": 0.87353515625}, {"start": 362.34, "end": 363.04, "word": " power", "probability": 0.8974609375}, {"start": 363.04, "end": 363.26, "word": " of", "probability": 0.9814453125}, {"start": 363.26, "end": 363.4, "word": " the", "probability": 0.92431640625}, {"start": 363.4, "end": 363.96, "word": " microscope", "probability": 0.85205078125}, {"start": 363.96, "end": 364.66, "word": " وقولّك", "probability": 0.6822509765625}, {"start": 364.66, "end": 365.32, "word": " كل", "probability": 0.337890625}, {"start": 365.32, "end": 365.52, "word": " ما", "probability": 0.8994140625}, {"start": 365.52, "end": 366.04, "word": " كانت", "probability": 0.99560546875}, {"start": 366.04, "end": 366.84, "word": " المسافة", "probability": 0.9776611328125}, {"start": 366.84, "end": 367.02, "word": " ما", "probability": 0.8828125}, {"start": 367.02, "end": 367.38, "word": " بين", "probability": 0.99462890625}, {"start": 367.38, "end": 368.2, "word": " جثمان", "probability": 0.76361083984375}, {"start": 368.2, "end": 369.06, "word": " مختلفان", "probability": 0.96533203125}, {"start": 369.06, "end": 369.42, "word": " وانا", "probability": 0.8234049479166666}, {"start": 369.42, "end": 369.84, "word": " قدرت", "probability": 0.8982747395833334}, {"start": 369.84, "end": 370.36, "word": " اميز", "probability": 0.828125}, {"start": 370.36, "end": 370.56, "word": " بين", "probability": 0.98974609375}, {"start": 370.56, "end": 370.96, "word": " هدول", "probability": 0.9422200520833334}, {"start": 370.96, "end": 371.7, "word": " الجثمان", "probability": 0.977783203125}, {"start": 371.7, "end": 371.94, "word": " كل", "probability": 0.97900390625}, {"start": 371.94, "end": 372.06, "word": " ما", "probability": 0.9521484375}, {"start": 372.06, "end": 372.34, "word": " كانت", 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"probability": 0.56298828125}, {"start": 965.26, "end": 965.76, "word": " نقطتان", "probability": 0.851806640625}, {"start": 965.76, "end": 966.36, "word": " المسافة", "probability": 0.9466552734375}, {"start": 966.36, "end": 966.78, "word": " بينهم", "probability": 0.92431640625}, {"start": 966.78, "end": 967.3, "word": " اكتر", "probability": 0.876220703125}, {"start": 967.3, "end": 967.84, "word": " قليلة", "probability": 0.9239908854166666}, {"start": 967.84, "end": 968.68, "word": " تمام؟", "probability": 0.6825358072916666}, {"start": 968.68, "end": 968.92, "word": " هذا", "probability": 0.79443359375}, {"start": 968.92, "end": 969.52, "word": " بالنسبة", "probability": 0.9764404296875}, {"start": 969.52, "end": 970.4, "word": " للعلاقة", "probability": 0.7744140625}, {"start": 970.4, "end": 971.16, "word": " الرياضية", "probability": 0.9029541015625}, {"start": 971.16, "end": 972.0, "word": " المضحة", "probability": 0.67425537109375}, {"start": 972.0, "end": 972.44, "word": " بتمنى", "probability": 0.6959228515625}, {"start": 972.44, "end": 972.88, "word": " تكون", "probability": 0.987548828125}, {"start": 972.88, "end": 973.64, "word": " واضحة", "probability": 0.9617919921875}, {"start": 973.64, "end": 973.84, "word": " كل", "probability": 0.8505859375}, {"start": 973.84, "end": 974.24, "word": " الأمور", "probability": 0.9855143229166666}, {"start": 974.24, "end": 974.6, "word": " تمام", "probability": 0.988525390625}], "temperature": 1.0}, {"id": 35, "seek": 101232, "start": 982.56, "end": 1012.32, "text": "الـ «depth of focus» معرفة ليها «thickness of field» «سمك المجال» اللي هو عمق المسافة أو عمق الرؤية معرفة ليها «thickness of field» اللي هو سمك المجال «as magnification increases depth of focus increases» إيش يعني سمك المجال أو سمك الـ field؟ معناه لو كان أنا عندي كتير طبقات لو عندي عينة فيها كتير طبقات أنا ماعملتلها تقطيع", "tokens": [6027, 39184, 4657, 25478, 295, 1879, 5933, 20449, 28480, 3660, 32239, 11296, 4657, 392, 618, 1287, 295, 2519, 5933, 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"word": " وعينة", "probability": 0.7420654296875}, {"start": 1016.52, "end": 1016.88, "word": " أخرى", "probability": 0.9596354166666666}, {"start": 1016.88, "end": 1017.2, "word": " كانت", "probability": 0.9619140625}, {"start": 1017.2, "end": 1017.78, "word": " تتكون", "probability": 0.9239908854166666}, {"start": 1017.78, "end": 1018.2, "word": " من", "probability": 0.9892578125}, {"start": 1018.2, "end": 1019.08, "word": " طبقتين", "probability": 0.9654541015625}, {"start": 1019.08, "end": 1019.72, "word": " كل", "probability": 0.8447265625}, {"start": 1019.72, "end": 1019.92, "word": " ما", "probability": 0.78759765625}, {"start": 1019.92, "end": 1020.38, "word": " كانت", "probability": 0.98486328125}, {"start": 1020.38, "end": 1021.08, "word": " الطبقات", "probability": 0.9791259765625}, {"start": 1021.08, "end": 1021.56, "word": " أقل", "probability": 0.9835611979166666}, {"start": 1021.56, "end": 1021.7, "word": " في", "probability": 0.927734375}, {"start": 1021.7, "end": 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105477, "start": 1026.71, "end": 1054.77, "text": "فبالتالي بيكون الـ Resolution وضوح الصورة في المنطقة اللي بيكون عندها الطبقات الأقل بتكون الـ Resolution عالية وبالتالي بتحتاج قوة تكبير عالية يبقى كل ما كانت الطبقات أقل يعني الـ Depth of Focus كان أليل كل ما كان الـ Resolution أكيد لما تكون الطبقات أليلة هيكون وضوح الصورة أكتر الـ Resolution أكتر فبالتالي الـ Magnification بيزيد يبقى العلاقة ما بين الـ Depth of Focus والمagnification علاقة عكسية", "tokens": [5172, 3555, 6027, 2655, 6027, 1829, 4724, 1829, 30544, 2423, 39184, 5015, 3386, 4032, 11242, 2407, 5016, 31767, 13063, 3660, 8978, 9673, 1863, 9566, 28671, 13672, 1829, 4724, 1829, 30544, 43242, 11296, 41950, 3555, 4587, 9307, 16247, 4587, 1211, 39894, 30544, 2423, 39184, 5015, 3386, 6225, 6027, 10632, 4032, 3555, 6027, 2655, 6027, 1829, 39894, 33753, 26108, 12174, 2407, 3660, 6055, 4117, 3555, 13546, 6225, 6027, 10632, 7251, 3555, 4587, 7578, 28242, 19446, 25961, 2655, 41950, 3555, 4587, 9307, 5551, 4587, 1211, 37495, 22653, 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40448, 1829, 2423, 39184, 441, 694, 8834, 8415, 318, 3386, 4032, 5551, 34740, 25124, 1829, 38207, 39894, 25957, 20292, 4386, 39894, 2304, 3794, 5016, 1829, 3224, 4724, 39184, 31562, 3926, 455, 6156, 3224, 1829, 4117, 44945, 30544, 16490, 1211, 9381, 8315, 20449, 42213, 2423, 39184, 5818, 2635, 13960, 16472, 13412, 16606, 21984, 6055, 30544, 28242, 9673, 30241, 20498, 9307, 4032, 46958, 5016, 3660, 20666, 1863, 35457, 3660, 5296, 24793], "avg_logprob": -0.2637140287769784, "compression_ratio": 1.6609589041095891, "no_speech_prob": 1.2516975402832031e-06, "words": [{"start": 1965.77, "end": 1966.37, "word": "حتى", "probability": 0.80712890625}, {"start": 1966.37, "end": 1966.63, "word": " إنك", "probability": 0.541748046875}, {"start": 1966.63, "end": 1966.95, "word": " تزيل", "probability": 0.9129231770833334}, {"start": 1966.95, "end": 1967.31, "word": " كل", "probability": 0.8330078125}, {"start": 1967.31, "end": 1968.51, "word": " القادورات", "probability": 0.76824951171875}, {"start": 1968.51, "end": 1969.01, "word": " عنها", "probability": 0.983154296875}, {"start": 1969.01, "end": 1969.63, "word": " أخر", "probability": 0.57952880859375}, {"start": 1969.63, "end": 1969.83, "word": " الشيء", "probability": 0.6211751302083334}, {"start": 1969.83, "end": 1970.09, "word": " الـ", "probability": 0.73779296875}, {"start": 1970.09, "end": 1970.45, "word": " Condenser", "probability": 0.6953125}, {"start": 1970.45, "end": 1970.85, "word": " أكيد", "probability": 0.9290364583333334}, {"start": 1970.85, "end": 1971.15, "word": " لما", "probability": 0.6304931640625}, {"start": 1971.15, "end": 1971.41, "word": " قبل", "probability": 0.811279296875}, {"start": 1971.41, "end": 1971.53, "word": " ما", "probability": 0.8671875}, {"start": 1971.53, "end": 1971.95, "word": " تنطفي", "probability": 0.7215576171875}, {"start": 1971.95, "end": 1972.45, "word": " بتدخل", "probability": 0.843994140625}, {"start": 1972.45, "end": 1972.73, "word": " فيه", "probability": 0.815673828125}, {"start": 1972.73, "end": 1973.25, "word": " شغلناه", "probability": 0.76689453125}, {"start": 1973.25, "end": 1973.83, "word": " بتنطفي", "probability": 0.7679443359375}, {"start": 1973.83, "end": 1974.09, "word": " الـ", "probability": 0.688720703125}, {"start": 1974.09, "end": 1974.61, "word": " Condenser", "probability": 0.84033203125}, {"start": 1974.61, "end": 1975.03, "word": " بـ", "probability": 0.849609375}, {"start": 1975.03, "end": 1975.53, "word": " Lentifree", "probability": 0.5108642578125}, {"start": 1975.53, "end": 1975.91, "word": " Cotton", "probability": 0.876953125}, {"start": 1975.91, "end": 1976.55, "word": " الـ", "probability": 0.577392578125}, {"start": 1976.55, "end": 1977.01, "word": " Swab", "probability": 0.791259765625}, {"start": 1977.01, "end": 1977.63, "word": " و", "probability": 0.62451171875}, {"start": 1977.63, "end": 1978.81, "word": " بتستخدمي", "probability": 0.8466796875}, {"start": 1978.81, "end": 1978.99, "word": " الـ", "probability": 0.9501953125}, {"start": 1978.99, "end": 1979.21, "word": " Lens", "probability": 0.783203125}, {"start": 1979.21, "end": 1979.71, "word": " Cleaning", "probability": 0.914306640625}, {"start": 1979.71, "end": 1980.29, "word": " Solution", "probability": 0.815673828125}, {"start": 1980.29, "end": 1980.51, "word": " و", "probability": 0.54541015625}, {"start": 1980.51, "end": 1980.75, "word": " أخر", "probability": 0.894775390625}, {"start": 1980.75, "end": 1981.11, "word": " الشيء", "probability": 0.9611002604166666}, {"start": 1981.11, "end": 1981.65, "word": " بتعملي", "probability": 0.9142252604166666}, {"start": 1981.65, "end": 1982.57, "word": " ..", "probability": 0.4638671875}, {"start": 1982.57, "end": 1983.33, "word": " بتمسحيه", "probability": 0.8857421875}, {"start": 1983.33, "end": 1983.61, "word": " بـ", "probability": 0.93798828125}, {"start": 1983.61, "end": 1984.07, "word": " Dry", "probability": 0.88037109375}, {"start": 1984.07, "end": 1985.69, "word": " Swab", "probability": 0.939453125}, {"start": 1985.69, "end": 1986.57, "word": " فهيك", "probability": 0.53106689453125}, {"start": 1986.57, "end": 1986.89, "word": " بنكون", "probability": 0.767578125}, {"start": 1986.89, "end": 1987.55, "word": " خلصنا", "probability": 0.988037109375}, {"start": 1987.55, "end": 1988.05, "word": " معمل", "probability": 0.931640625}, {"start": 1988.05, "end": 1988.29, "word": " الـ", "probability": 0.6885986328125}, {"start": 1988.29, "end": 1989.05, "word": " Microscope", "probability": 0.8238932291666666}, {"start": 1989.05, "end": 1989.77, "word": " ان", "probability": 0.52392578125}, {"start": 1989.77, "end": 1990.01, "word": " شاء", "probability": 0.922119140625}, {"start": 1990.01, "end": 1990.03, "word": " الله", "probability": 0.9443359375}, {"start": 1990.03, "end": 1990.47, "word": " تكون", "probability": 0.9677734375}, {"start": 1990.47, "end": 1990.87, "word": " كل", "probability": 0.98486328125}, {"start": 1990.87, "end": 1991.61, "word": " المعلومات", "probability": 0.98583984375}, {"start": 1991.61, "end": 1991.91, "word": " واضحة", "probability": 0.98779296875}, {"start": 1991.91, "end": 1992.33, "word": " بالنسبة", "probability": 0.9931640625}, {"start": 1992.33, "end": 1992.73, "word": " لكم", "probability": 0.686279296875}], "temperature": 1.0}, {"id": 73, "seek": 201044, "start": 1993.16, "end": 2010.44, "text": "هيكون فينا مناقشة الأسبوع اللي جاي يوم التلاتة الساعة أحد عشر هيكون تكون المناقشة في معمل الـ Microscope ومعمل الـ Introduction إذا في أي معلومات إن شاء الله هنقشها في هذا اللقاء السلام عليكم ورحمة الله وبركاته", "tokens": [3224, 1829, 30544, 8978, 8315, 3714, 8315, 4587, 8592, 3660, 16247, 35457, 45367, 13672, 1829, 10874, 47302, 7251, 20498, 16712, 1211, 9307, 3660, 21136, 995, 27884, 5551, 24401, 6225, 46309, 39896, 30544, 6055, 30544, 9673, 8315, 4587, 8592, 3660, 8978, 20449, 42213, 2423, 39184, 5818, 2635, 13960, 4032, 2304, 25957, 1211, 2423, 39184, 27193, 882, 11933, 15730, 8978, 36632, 20449, 1211, 20498, 9307, 36145, 13412, 16606, 21984, 8032, 1863, 4587, 8592, 11296, 8978, 23758, 13672, 4587, 16606, 21136, 37440, 25894, 24793, 4032, 2288, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224], "avg_logprob": -0.16134511566032533, "compression_ratio": 1.7920792079207921, "no_speech_prob": 3.49879264831543e-05, "words": [{"start": 1993.16, "end": 1993.64, "word": "هيكون", "probability": 0.95263671875}, {"start": 1993.64, "end": 1994.04, "word": " فينا", "probability": 0.772705078125}, {"start": 1994.04, "end": 1995.0, "word": " مناقشة", "probability": 0.95791015625}, {"start": 1995.0, "end": 1996.18, "word": " الأسبوع", "probability": 0.9791666666666666}, {"start": 1996.18, "end": 1996.34, "word": " اللي", "probability": 0.79345703125}, {"start": 1996.34, "end": 1996.56, "word": " جاي", "probability": 0.8173828125}, {"start": 1996.56, "end": 1996.8, "word": " يوم", "probability": 0.853515625}, {"start": 1996.8, "end": 1997.4, "word": " التلاتة", "probability": 0.879638671875}, {"start": 1997.4, "end": 1997.74, "word": " الساعة", "probability": 0.9501953125}, {"start": 1997.74, "end": 1997.9, "word": " أحد", "probability": 0.39886474609375}, {"start": 1997.9, "end": 1998.32, "word": " عشر", "probability": 0.82080078125}, {"start": 1998.32, "end": 1999.24, "word": " هيكون", "probability": 0.57275390625}, {"start": 1999.24, "end": 1999.62, "word": " تكون", "probability": 0.6461181640625}, {"start": 1999.62, "end": 2000.22, "word": " المناقشة", "probability": 0.9958984375}, {"start": 2000.22, "end": 2000.34, "word": " في", "probability": 0.95361328125}, {"start": 2000.34, "end": 2000.64, "word": " معمل", "probability": 0.7373046875}, {"start": 2000.64, "end": 2000.96, "word": " الـ", "probability": 0.81005859375}, {"start": 2000.96, "end": 2001.56, "word": " Microscope", "probability": 0.7378743489583334}, {"start": 2001.56, "end": 2002.5, "word": " ومعمل", "probability": 0.78125}, {"start": 2002.5, "end": 2002.78, "word": " الـ", "probability": 0.916015625}, {"start": 2002.78, "end": 2003.52, "word": " Introduction", "probability": 0.845703125}, {"start": 2003.52, "end": 2003.76, "word": " إذا", "probability": 0.86083984375}, {"start": 2003.76, "end": 2003.9, "word": " في", "probability": 0.87451171875}, {"start": 2003.9, "end": 2004.08, "word": " أي", "probability": 0.82763671875}, {"start": 2004.08, "end": 2004.78, "word": " معلومات", "probability": 0.9918212890625}, {"start": 2004.78, "end": 2005.52, "word": " إن", "probability": 0.455078125}, {"start": 2005.52, "end": 2005.7, "word": " شاء", "probability": 0.949462890625}, {"start": 2005.7, "end": 2005.84, "word": " الله", "probability": 0.96484375}, {"start": 2005.84, "end": 2006.58, "word": " هنقشها", "probability": 0.95546875}, {"start": 2006.58, "end": 2006.92, "word": " في", "probability": 0.98876953125}, {"start": 2006.92, "end": 2007.36, "word": " هذا", "probability": 0.896484375}, {"start": 2007.36, "end": 2007.94, "word": " اللقاء", "probability": 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\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/bxthOW-TT6Y.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/bxthOW-TT6Y.srt
new file mode 100644
index 0000000000000000000000000000000000000000..4c7a223d2ddc8dbef1eb9556d11a026e6e166e7f
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/bxthOW-TT6Y.srt
@@ -0,0 +1,2093 @@
+1
+00:00:02,680 --> 00:00:06,220
+السلام عليكم ورحمة الله وبركاته، نستكمل معكم
+
+2
+00:00:06,220 --> 00:00:09,560
+محاضرات مساق  الـ Microbiology العملية في هذا
+
+3
+00:00:09,560 --> 00:00:12,720
+اللقاء، سنتكلم عن Media Preparation and
+
+4
+00:00:12,720 --> 00:00:16,060
+Sterilization، Aseptic Techniques، Pure Culture
+
+5
+00:00:16,060 --> 00:00:20,220
+Concept. سنتعرف على طريقة تحضير الـ Media، لـ
+
+6
+00:00:20,220 --> 00:00:23,420
+Sterilization، التعقيم، كيف بدي أعقم الأدوات التي
+
+7
+00:00:23,420 --> 00:00:26,940
+أستخدمها في معمل الـ Microbiology. طبعاً الـ
+
+8
+00:00:26,940 --> 00:00:29,920
+Sterilization يعتبر من الـ Aseptic Techniques، الـ
+
+9
+00:00:29,920 --> 00:00:33,570
+Aseptic Techniques هي كل الإجراءات التي أنا أتبَعُها
+
+10
+00:00:33,570 --> 00:00:37,690
+حتى أمنع الـ contamination. الـ pure culture هي
+
+11
+00:00:37,690 --> 00:00:42,410
+المزرعة التي تكون المستعمرات الكولونية أصلها خلية
+
+12
+00:00:42,410 --> 00:00:46,210
+بكتيرية واحدة، يعني ألاحظ على الطبق أن المستعمرة
+
+13
+00:00:46,210 --> 00:00:52,050
+كلها نفس الـ morphology. هذه المحاضرة سنتعرف على كل
+
+14
+00:00:52,050 --> 00:00:53,950
+من هذه المواضيع بالتفصيل.
+
+15
+00:00:59,080 --> 00:01:02,480
+الـ Natural Environment. Microorganism usually
+
+16
+00:01:02,480 --> 00:01:06,720
+exists as Mixed Population. ماذا يعني Mixed
+
+17
+00:01:06,720 --> 00:01:10,860
+Population؟ لو أنا جبت السواب، طبعاً السواب أستخدمها
+
+18
+00:01:10,860 --> 00:01:15,580
+لأخذ مسحات، أخذت بالسواب مسحة من الطاولة وزرعتها
+
+19
+00:01:15,580 --> 00:01:19,280
+عليها على وسط غذائي، بعدين حطيت الطبق في الـ
+
+20
+00:01:19,280 --> 00:01:23,700
+incubator لمدة 24 ساعة، يعني عملت incubation. الـ
+
+21
+00:01:23,700 --> 00:01:26,960
+incubator طبعاً هيوفر الظروف المناسبة لنمو
+
+22
+00:01:26,960 --> 00:01:32,030
+البكتيريا. بعد 24 ساعة، سأجد بأن فيه نمو على الطبق،
+
+23
+00:01:32,030 --> 00:01:36,570
+سأجد كولوني، هذه الكولوني ستكون مختلفة في الـ
+
+24
+00:01:36,570 --> 00:01:41,170
+morphology، يعني سأجد مجموعة من الـ microorganism
+
+25
+00:01:41,170 --> 00:01:47,390
+mixed microorganism أو mixed population، وليس كائن
+
+26
+00:01:47,390 --> 00:01:50,770
+واحد. However, we are
+
+27
+00:01:58,510 --> 00:02:03,410
+عشان أدرس خصائص هذه الكائنات وأتعرف عليها، لازم
+
+28
+00:02:03,410 --> 00:02:08,930
+آخذ كل كائن لحال، لازم يكون عندي a pure culture.  شو
+
+29
+00:02:08,930 --> 00:02:13,270
+يعني a pure culture؟ A pure culture is one in
+
+30
+00:02:13,270 --> 00:02:17,430
+which all organisms are descendants of the same
+
+31
+00:02:17,430 --> 00:02:21,840
+organism. هي المزرعة التي تكون المستعمرات عليها من
+
+32
+00:02:21,840 --> 00:02:27,540
+نفس السلالة، يعني أصلها كائن واحد، يعني أصلها خلية
+
+33
+00:02:27,540 --> 00:02:32,260
+بكتيرية واحدة. Techniques for obtaining pure culture
+
+34
+00:02:32,260 --> 00:02:36,380
+from a mixed population will be described in this
+
+35
+00:02:36,380 --> 00:02:40,980
+slide. لو أنا أخذت عينة natural  ورحت زراعتها،
+
+36
+00:02:40,980 --> 00:02:45,960
+بعد 24 ساعة، سأجد عندي مستعمرات حجمها كبير،
+
+37
+00:02:45,960 --> 00:02:49,610
+ومستعمرات حجمها صغير، يبقى هنا عندي mixed
+
+38
+00:02:49,610 --> 00:02:54,610
+population. عشان أعمل identification لكل واحدة، لازم
+
+39
+00:02:54,610 --> 00:02:59,310
+أعمل pure culture. هذا المعمل سنتعرف كيف أنا بدي
+
+40
+00:02:59,310 --> 00:03:03,530
+أعمل pure culture عشان أتعرف على كل بكتيريا على حِدَة
+
+41
+00:03:03,530 --> 00:03:07,510
+من الـ mixed population التي أنا زرعتها في الأول.
+
+42
+00:03:13,340 --> 00:03:17,040
+In working with microorganisms, we must have a
+
+43
+00:03:17,040 --> 00:03:20,980
+method of transferring a growing organism from a
+
+44
+00:03:20,980 --> 00:03:23,680
+pure culture to a sterile medium without
+
+45
+00:03:23,680 --> 00:03:28,460
+introducing any unwanted outside contaminants. خلال
+
+46
+00:03:28,460 --> 00:03:32,160
+الشغل في معمل الـ Microbiology، لازم يكون في عندي طرق
+
+47
+00:03:32,160 --> 00:03:36,480
+وإجراءات لمنع الـ contamination. في حال نقلت الـ
+
+48
+00:03:36,480 --> 00:03:41,040
+microorganism من pure culture إلى sterile media
+
+49
+00:03:41,040 --> 00:03:45,220
+يعني لوحة ثانية. لو كان في عندي pure culture على
+
+50
+00:03:45,220 --> 00:03:50,360
+ سبيل المثال E.coli، وبدي أزرعها على وسط ثاني، لازم
+
+51
+00:03:50,360 --> 00:03:53,540
+ألتزم بكل الطرق والإجراءات لمنع الـ contamination
+
+52
+00:03:53,540 --> 00:03:59,000
+خلال عملية النقل، أو حتى لما بدي أنقل micro
+
+53
+00:03:59,000 --> 00:04:02,780
+organism من mixed population إلى pure culture، لازم
+
+54
+00:04:02,780 --> 00:04:06,880
+ألتزم بالإجراءات لمنع الـ contamination. من ضمن هذه
+
+55
+00:04:06,880 --> 00:04:09,640
+الإجراءات أني أنا أمسح الـ bench بالكحول قبل ما
+
+56
+00:04:09,640 --> 00:04:14,500
+أبدأ الشغل. لو استخدمت inoculating loop، تم تعقيمه قبل ما
+
+57
+00:04:14,500 --> 00:04:19,280
+أنا أستخدمه، أحرقه بجوار اللهب. كمان كل أدواتي التي
+
+58
+00:04:19,280 --> 00:04:24,360
+أستخدمها تكون معقمة. من loop، Media، إلى الشابكة، سنتعرف
+
+59
+00:04:24,360 --> 00:04:28,160
+في هذه المحاضرة على طرق تعقيم كل أداة في معمل الـ
+
+60
+00:04:28,160 --> 00:04:32,460
+Microbiology. This method of preventing unwanted
+
+61
+00:04:32,460 --> 00:04:36,820
+microorganisms. بقول لك إن هذه الإجراءات التي أطمئن
+
+62
+00:04:36,820 --> 00:04:40,540
+فيها إن الوسط ما يصير فيه تلوث، ما يصير contamination،
+
+63
+00:04:40,540 --> 00:04:44,300
+بسميها الـ Aseptic technique. يبقى الـ
+
+64
+00:04:44,300 --> 00:04:47,920
+Aseptic technique هي كل الإجراءات التي يتم
+
+65
+00:04:47,920 --> 00:04:56,540
+اتخاذها لمنع الـ contamination. For
+
+66
+00:04:56,540 --> 00:05:00,420
+the most part، البكتيريا، وفيزيولوجيتها، only can be
+
+67
+00:05:00,420 --> 00:05:04,400
+studied in pure culture. عشان أدرس خصائص البكتيريا،
+
+68
+00:05:04,400 --> 00:05:08,940
+لازم يكون عندي pure culture، مش mixed population.
+
+69
+00:05:08,940 --> 00:05:13,140
+The best way to obtain a pure culture is to start
+
+70
+00:05:13,140 --> 00:05:17,380
+with a single bacterial cell. عشان أحصل على pure
+
+71
+00:05:17,380 --> 00:05:22,450
+culture، لازم أبدأ بـ single bacterial cell. ماذا يعني
+
+72
+00:05:22,450 --> 00:05:25,910
+single bacterial cell؟ يعني isolated colony، لأن الـ
+
+73
+00:05:25,910 --> 00:05:30,470
+isolated colony أصلها خلية بكتيرية واحدة. فلما بدي
+
+74
+00:05:30,470 --> 00:05:33,870
+أعمل pure culture من mixed population، بروح بزرع
+
+75
+00:05:33,870 --> 00:05:37,990
+بطريقة الربع، آخذ الـ isolated colony من الربع
+
+76
+00:05:37,990 --> 00:05:43,470
+الرابع. هيك بضمن أن الـ culture يكون pure، لأن أصلها
+
+77
+00:05:43,470 --> 00:05:46,650
+الـ isolated colony خلية بكتيرية واحدة.
+
+78
+00:05:52,950 --> 00:05:57,970
+الخلية تنقسم بسرعة، تنتج ملايين من الخلايا خلال 24
+
+79
+00:05:57,970 --> 00:06:03,550
+ساعة. يبقى بعد 24 ساعة سأجد عندي ملايين من الخلايا
+
+80
+00:06:03,550 --> 00:06:08,270
+البكتيرية. طب لو أنا ما اتبعت الـ Aseptic Technique،
+
+81
+00:06:08,270 --> 00:06:12,810
+وكان عندي contaminant unwanted غير التي أنا بدي
+
+82
+00:06:12,810 --> 00:06:17,490
+إياها، سينمو الـ unwanted، الـ contaminant، وينقسم ويصير
+
+83
+00:06:17,490 --> 00:06:21,750
+فيه عندي contamination. يبقى الـ culture هيصير mixed
+
+84
+00:06:21,750 --> 00:06:26,690
+population، مش pure culture. A single unwanted
+
+85
+00:06:26,690 --> 00:06:31,170
+contaminant can do the same thing in an otherwise
+
+86
+00:06:31,170 --> 00:06:36,050
+pure culture, making the culture useless. يبقى صار
+
+87
+00:06:36,050 --> 00:06:39,470
+عندي mixed population، يبقى الـ culture بتصير
+
+88
+00:06:39,470 --> 00:06:44,750
+useless، غير صالحة للاستخدام. For this reason,
+
+89
+00:06:44,750 --> 00:06:49,610
+أعتقد أن، I guess the restricted trial procedure must
+
+90
+00:06:49,610 --> 00:06:53,410
+be used. عشان أعمل identification، لازم يكون عندي
+
+91
+00:06:53,410 --> 00:06:57,750
+pure culture وليس mixed population. عشان هيك لازم
+
+92
+00:06:57,750 --> 00:07:02,030
+يكون فيه إجراءات صارمة عشان أمنع الـ contamination.
+
+93
+00:07:02,030 --> 00:07:06,650
+عشان
+
+94
+00:07:06,650 --> 00:07:10,870
+أزرع وأنّمي الـ microorganism، لازم يكون عندي وسط
+
+95
+00:07:10,870 --> 00:07:16,630
+غذائي لنمو البكتيريا. بسمي هذا الوسط الـ media. الـ
+
+96
+00:07:16,630 --> 00:07:20,870
+media هي الوسط الغذائي لنمو البكتيريا. In working
+
+97
+00:07:20,870 --> 00:07:24,810
+with microorganisms, we must also have a sterile
+
+98
+00:07:24,810 --> 00:07:28,410
+nutrient-containing media in which to grow the
+
+99
+00:07:28,410 --> 00:07:32,530
+organism. طبعاً الـ media لازم تكون sterile، لازم تكون
+
+100
+00:07:32,530 --> 00:07:37,370
+معقمة. وهذا من الـ aseptic technique. Anything in or
+
+101
+00:07:37,370 --> 00:07:41,490
+on which we grow a microorganism is termed a medium.
+
+102
+00:07:41,490 --> 00:07:45,670
+بقول لي أي شيء أنمي فيه الـ microorganism بسميه medium.
+
+103
+00:07:45,670 --> 00:07:50,890
+الـ medium مفرد، جمعها media. A nutrient blend، الـ
+
+104
+00:07:50,890 --> 00:07:54,790
+blend يعني خليط، used to support microbial growth.
+
+105
+00:07:54,790 --> 00:07:59,670
+هي خليط من المواد الغذائية لنمو الـ microorganism.
+
+106
+00:07:59,670 --> 00:08:04,910
+Dehydrated powder according to manufacturer's
+
+107
+00:08:04,910 --> 00:08:10,390
+instructions. بقول لي هذه الميديا بتكون بودرة، كيف أنا
+
+108
+00:08:10,390 --> 00:08:15,630
+بدي أحضرها؟ بحلها بـ distilled water. Dehydrated يعني البودرة
+
+109
+00:08:15,630 --> 00:08:20,290
+بدي أحلها بـ distilled water حسب التعليمات الموجودة على
+
+110
+00:08:20,290 --> 00:08:25,290
+الزجاجة. على سبيل المثال، Nutrient agar بتيجي بزجاجة
+
+111
+00:08:25,290 --> 00:08:32,570
+مكتوب عليها كل 28 غراماً أحلها بـ 1 لتر distilled water.
+
+112
+00:08:32,570 --> 00:08:36,930
+طبعاً حسب الكمية التي أنا أحضرها، لو بدي أحضر 500 مل،
+
+113
+00:08:37,200 --> 00:08:41,800
+أحسب كم من غرام بدي من البودرة، وأحلها بـ 500 مل
+
+114
+00:08:41,800 --> 00:08:47,920
+distilled water. Agar is a dried hydrophilic jelly-like
+
+115
+00:08:47,920 --> 00:08:52,960
+substance. بقول لي الأجار، قلنا عبارة عن solidifying
+
+116
+00:08:52,960 --> 00:08:58,320
+agent، هو مادة مجففة زي الـ jelly، hydrophilic، يحب الماء،
+
+117
+00:08:58,320 --> 00:09:02,840
+يعطي طبعاً الـ media القوام الصلب عشان ما
+
+118
+00:09:02,840 --> 00:09:07,450
+يكون سائلاً. Extracted from various species of red
+
+119
+00:09:07,450 --> 00:09:11,270
+algae. بقول لك بيستخلص من كائنات مختلفة من الطحالب
+
+120
+00:09:11,270 --> 00:09:16,210
+الحمراء. It's used as a solidifying agent. قلنا هو
+
+121
+00:09:16,210 --> 00:09:21,530
+يُعتبر solidifying agent عشان يُصلّب الـ media. الأجار
+
+122
+00:09:21,530 --> 00:09:27,170
+يذوب في درجة حرارة 85، ويتصلّب عند درجة حرارة 40.
+
+123
+00:09:33,040 --> 00:09:38,300
+Gelatin is a colorless and odorless substance. شو هو
+
+124
+00:09:38,300 --> 00:09:42,140
+الجيلاتين؟ الجيلاتين مادة ليس لها رائحة وليس لها
+
+125
+00:09:42,140 --> 00:09:46,080
+لون. طيب شو علاقتها بالميديا؟ قلنا زمان كانوا
+
+126
+00:09:46,080 --> 00:09:50,580
+يستخدمونه كـ solidifying agent، لكن حالياً ما يتم
+
+127
+00:09:50,580 --> 00:09:54,820
+استخدامه، تم استبداله بالأجار. طيب ليش تم استبداله ولماذا
+
+128
+00:09:54,820 --> 00:09:58,860
+ما يتم استخدامه حالياً؟ لأن في بعض أنواع البكتيريا
+
+129
+00:09:58,860 --> 00:10:03,960
+تُفرز أنزيم يُسمّى gelatinase، من اسمه يُكسر الجيلاتين،
+
+130
+00:10:03,960 --> 00:10:08,680
+وبالتالي يتحول الوسط إلى سائل. That is made from the
+
+131
+00:10:08,680 --> 00:10:13,240
+collagen. مصنوع من الكولاجين. يذوب عند درجة حرارة 35
+
+132
+00:10:13,240 --> 00:10:19,520
+درجة مئوية. في الـ autoclave، ويتطلب درجات حرارة أقل. Micro
+
+133
+00:10:19,520 --> 00:10:23,000
+organisms are grown and subcultured on different
+
+134
+00:10:23,000 --> 00:10:26,440
+types of chemical media. بقول إن الـ microorganism
+
+135
+00:10:26,440 --> 00:10:30,960
+بنمّيها وبَعمل لها subculture على أوساط غذائية مختلفة.
+
+136
+00:10:31,600 --> 00:10:35,560
+Subculture، شو يعني subculture؟ وشو الفرق بينها
+
+137
+00:10:35,560 --> 00:10:40,200
+وبين الـ culture؟ لو أنا أخذت عينة جديدة وزرعتها
+
+138
+00:10:40,200 --> 00:10:44,640
+على وسط، بحكي culture. لكن لو زرعتها كمان مرة على
+
+139
+00:10:44,640 --> 00:10:49,480
+طبق ثاني، بحكي subculture. الـ media، بقول لي ممكن تكون
+
+140
+00:10:49,480 --> 00:10:52,420
+chemically defined. شو يعني chemically defined؟
+
+141
+00:10:52,420 --> 00:10:57,100
+يعني known concentration. مكونات الـ media كمياتها
+
+142
+00:10:57,100 --> 00:11:02,440
+معروفة. ممكن تكون الـ media chemically undefined،
+
+143
+00:11:02,440 --> 00:11:07,480
+النسب تَبَعُتها مش معروفة، unknown proportion. A medium
+
+144
+00:11:07,480 --> 00:11:12,700
+is sterilized. بمعنى sterilization يعني living
+
+145
+00:11:12,700 --> 00:11:17,820
+organisms removed. الـ media، قلنا كل أدواتي يتم
+
+146
+00:11:17,820 --> 00:11:21,240
+تعقيمها، والـ media لازم أني أنا أعقمها، من الـ
+
+147
+00:11:21,240 --> 00:11:26,280
+aseptic technique، أني أنا أعقمها. طرق تعقيم الـ
+
+148
+00:11:26,280 --> 00:11:29,700
+media، طبعاً فيه أكثر من طريقة لتعقيم الـ media.
+
+149
+00:11:29,700 --> 00:11:33,340
+قبل ما نتكلم عن طرق تعقيم الـ media، سنتكلم كيف أنا
+
+150
+00:11:33,340 --> 00:11:39,600
+بدي أحضر media. لو بدي أحضر الـ Nutrient agar، أقرأ
+
+151
+00:11:39,600 --> 00:11:42,780
+طبعاً التعليمات الموجودة على الزجاجة. بتيجي
+
+152
+00:11:42,780 --> 00:11:45,920
+بزجاجة مكتوب عليها Nutrient agar، هذه الزجاجة
+
+153
+00:11:45,920 --> 00:11:50,900
+powder. أقرأ التعليمات، أوزن حسب الكمية التي أنا بدي
+
+154
+00:11:50,900 --> 00:11:55,920
+إياها. بعد ما أوزن، أحط الوزن في flask، أضيف distilled
+
+155
+00:11:55,920 --> 00:12:00,800
+water حسب الكمية، وأحرّكها، بعدين أحطها على الـ hot plate،
+
+156
+00:12:00,800 --> 00:12:05,420
+فيه زر للحرارة، وزر للسرعة. طبعاً قبلها
+
+157
+00:12:05,420 --> 00:12:09,780
+بنحط في الـ flask مغناطيس حتى تذوب البودرة. لما
+
+158
+00:12:09,780 --> 00:12:13,940
+تذوب منيح الـ media، أدخل الـ flask على الـ autoclave.
+
+159
+00:12:13,940 --> 00:12:18,700
+الـ autoclave هيعقم لي الـ media، ويقتل كل الـ
+
+160
+00:12:18,700 --> 00:12:25,150
+microorganism، بما فيها الـ spores. هاك الـ media طلعت
+
+161
+00:12:25,150 --> 00:12:29,050
+معقمة. هذه من الـ aseptic technique. فيه طرق أخرى
+
+162
+00:12:29,050 --> 00:12:34,370
+منها الـ oven، filtration، UV exposure، ethylene oxide، لكن
+
+163
+00:12:34,370 --> 00:12:38,070
+الـ most common التي يتم استخدامها الـ autoclave.
+
+164
+00:12:38,070 --> 00:12:44,430
+Other sterilization methods، طرق أخرى
+
+165
+00:12:44,430 --> 00:12:48,990
+للتعقيم،  filtration،  عشان التوحش، Chemicals، مواد
+
+166
+00:12:48,990 --> 00:12:52,270
+الكيميائية، Ozone، nitrogen dioxide، hydrogen
+
+167
+00:12:52,270 --> 00:12:55,770
+peroxide. هذا الـ autoclave used for sterilization،
+
+168
+00:12:55,770 --> 00:13:01,110
+أعقم من خلاله الـ media، يتم قتل كل الـ micro
+
+169
+00:13:01,110 --> 00:13:06,010
+organism، بما فيها الـ spores، تحت درجة حرارة عالية وضغط
+
+170
+00:13:06,010 --> 00:13:10,730
+مرتفع. هذا هو الـ UV exposure، الـ UV بتعمل على قتل
+
+171
+00:13:10,730 --> 00:13:14,990
+الـ microorganism.  بالنسبة لهذه الطريقة هي الـ
+
+172
+00:13:14,990 --> 00:13:19,420
+filtration. بكون محضّر media، وأحطها بـ cup، وأسحب
+
+173
+00:13:19,420 --> 00:13:23,820
+الـ media بالسرنجة، وأروح بلزّق filter membrane زي
+
+174
+00:13:23,820 --> 00:13:27,940
+الذي بالصورة، هذه هي filter membrane، بالسرنجة، وبنزل
+
+175
+00:13:27,940 --> 00:13:32,780
+شوّية شوّية، هيك بتتفلتر الـ media، وأكيد بنزلها في الـ
+
+176
+00:13:32,780 --> 00:13:37,920
+sterile cup. من الأمثلة على الـ media التي أستخدم
+
+177
+00:13:37,920 --> 00:13:42,660
+طريقة الـ filtration لتعقيمها، الـ urea agar، الـ urea
+
+178
+00:13:42,660 --> 
+
+223
+00:17:13,880 --> 00:17:18,720
+a bacterial unit that can be seen with the naked eye
+
+224
+00:17:18,720 --> 00:17:22,220
+هي أصغر وحدة بكتيرية ممكن أنا أشوفها، قولنا الـ
+
+225
+00:17:22,220 --> 00:17:25,780
+isolated colony بحصل عليها من الربع الرابع لو أنا
+
+226
+00:17:25,780 --> 00:17:30,780
+زرعت بطريقة الأربع أرباع  culture لو إجتني عينة يورينز
+
+227
+00:17:30,780 --> 00:17:34,320
+زرعتها أول مرة بسميها primary culture يبقى هي جزء من
+
+228
+00:17:34,320 --> 00:17:38,880
+العينة، is part of a specimen، الـ  ground بنميها in
+
+229
+00:17:38,880 --> 00:17:43,220
+the cultural media بيتم زراعتها و تنميتها على the cultural
+
+230
+00:17:43,220 --> 00:17:47,360
+media، طيب شو تعريف الـ  cultural media؟ it is a medium
+
+231
+00:17:47,360 --> 00:17:52,880
+هو وسط liquid أو سائل that contains nutrients to
+
+232
+00:17:52,880 --> 00:17:57,120
+grow bacteria in vitro، فيه مغذيات لازمة لنمو
+
+233
+00:17:57,120 --> 00:18:01,780
+البكتيريا، لازم أزرع و أنمي البكتيريا فين؟ دي
+
+234
+00:18:01,780 --> 00:18:05,860
+سببين، السبب الأول، sometimes we cannot identify with
+
+235
+00:18:05,860 --> 00:18:09,820
+a microscopical examination directly، لأنه بالـ direct
+
+236
+00:18:09,820 --> 00:18:13,540
+examination، اسمه direct، بجيب العينة اللي بدي
+
+237
+00:18:13,540 --> 00:18:17,860
+أبحثها، على سبيل المثال عينة urine، بـ centrifugeها، باخد
+
+238
+00:18:17,860 --> 00:18:20,960
+الـ sediments، بشوف تحت الـ microscope، هذا اسمه
+
+239
+00:18:20,960 --> 00:18:24,800
+direct examination، فقط هشوف عندي بكتيريا أو لأ،
+
+240
+00:18:24,800 --> 00:18:29,440
+ممكن أتعرف على شكلها، أو على هل هي gram positive أو
+
+241
+00:18:29,440 --> 00:18:32,780
+gram negative، من خلال صباغة الـ slide، لكن ما بقدر
+
+242
+00:18:32,780 --> 00:18:38,660
+أتعرف بشكل نهائي على البكتيريا، لازم أعزلها وأنميها،
+
+243
+00:18:38,660 --> 00:18:43,060
+وأجري عليها فحوصات كيميائية،  للتعريف
+
+244
+00:18:43,060 --> 00:18:47,960
+النهائي، السبب الثاني، sometimes we do culture for
+
+245
+00:18:47,960 --> 00:18:52,160
+antibiotic sensitivity testing،  لأن بعد ما أعرف أي
+
+246
+00:18:52,160 --> 00:18:57,490
+بكتيريا، لازم أعمل فحص المضادات الحيوية، عشان أشوف هي
+
+247
+00:18:57,490 --> 00:19:02,730
+البكتيريا لأي مضاد حيوي هتستجيب، عشان الدكتور يوصف
+
+248
+00:19:02,730 --> 00:19:06,590
+العلاج للمريض، في الفحص ده، بعتمد أنه أنا بدي أجيب
+
+249
+00:19:06,590 --> 00:19:11,730
+البكتيريا اللي نميتها، وعرفتها، بزراها على طبق بشكل
+
+250
+00:19:11,730 --> 00:19:15,770
+continuous، بدي أتأكد أن الطبق كله زرعت عليه
+
+251
+00:19:15,770 --> 00:19:20,870
+بكتيريا، بعدها أحط أقراص Antibiotic فوق البكتيريا
+
+252
+00:19:20,870 --> 00:19:25,070
+اللي أنا زرعتها، بحطها في الـ incubator، بعد 24 ساعة
+
+253
+00:19:25,070 --> 00:19:30,090
+بشوف النتيجة، إذا كانت البكتيريا حساسة للـ Antibiotic،
+
+254
+00:19:30,090 --> 00:19:34,370
+بشوف zone of inhibition، منطقة فاضية حوالين الـ
+
+255
+00:19:34,370 --> 00:19:37,770
+Antibiotic، يعني الـ Antibiotic قتل هذه البكتيريا،
+
+256
+00:19:37,770 --> 00:19:44,330
+لو كانت مقاومة للـ Antibiotic، هشوف نمو البكتيريا
+
+257
+00:19:44,330 --> 00:19:48,210
+حول الـ Antibiotic، أكيد الدكتور هيختار الـ
+
+258
+00:19:48,210 --> 00:19:52,870
+Antibiotic اللي كانت البكتيريا حساسة له،  أو البكتيريا اللي كانت
+
+259
+00:19:52,870 --> 00:19:57,770
+حساسة للـ Antibiotic، مش المقاومة، لأنه مش هيستجيب له،
+
+260
+00:19:57,770 --> 00:20:01,870
+خواص الـ Media، أكيد الـ Media بتدعم نمو
+
+261
+00:20:01,870 --> 00:20:07,050
+البكتيريا، بتحتوي على مواد غذائية،  الـ pH للميديا
+
+262
+00:20:07,050 --> 00:20:11,550
+بيكون neutral to slightly alkaline، درجة الحرارة
+
+263
+00:20:11,550 --> 00:20:17,130
+لازم تكون مناسبة، لأن البكتيريا بتنمو عند درجة حرارة
+
+264
+00:20:17,130 --> 00:20:17,450
+37
+
+265
+00:20:20,740 --> 00:20:25,080
+بشوف zone of inhibition حوالين الـ antibiotic، يعني
+
+266
+00:20:25,080 --> 00:20:29,960
+الـ antibiotic قتل البكتيريا، high معناها ان هي
+
+267
+00:20:29,960 --> 00:20:34,840
+حساسة، لكن لو كانت مقاومة لـ antibiotic معين، هشوف
+
+268
+00:20:34,840 --> 00:20:38,340
+نمو البكتيريا حول الـ antibiotic، هان في نمو
+
+269
+00:20:38,340 --> 00:20:41,620
+حول الـ antibiotic، يبقى مقاومة، لكن هان zone of
+
+270
+00:20:41,620 --> 00:20:46,720
+inhibition، يبقى حساسة، فالدكتور رح يختار الـ
+
+271
+00:20:46,720 --> 00:20:52,720
+Antibiotic الحساس، مش المقاوم، لأنه مش هيستجيب، إذن
+
+272
+00:20:52,720 --> 00:20:58,980
+الـ
+
+273
+00:20:58,980 --> 00:21:02,200
+media بنصنفها بناء على ثلاث معايير، حسب الـ
+
+274
+00:21:02,200 --> 00:21:05,680
+consistency، حسب القوام، الـ nutrition، المواد
+
+275
+00:21:05,680 --> 00:21:09,660
+الغذائية، الـ function، الوظيفة، بالنسبة للقوام، بنصنفها
+
+276
+00:21:09,660 --> 00:21:15,930
+لـ solid، semi-solid،  liquid، نسبة الأجار بتكون من 1.5%
+
+277
+00:21:15,930 --> 00:21:20,510
+لـ 2% أجار، من الأمثلة عليها لـ  Nutrient agar، أجار الـ
+
+278
+00:21:20,510 --> 00:21:24,090
+chemical lead، نسبة الأجار بتكون 4 لـ 10%، من
+
+279
+00:21:24,090 --> 00:21:28,110
+الأمثلة عليها الـ media SIM، أو اللي استخدمناها في
+
+280
+00:21:28,110 --> 00:21:33,050
+فحص  المجتلد، الـ broth ما فيها أجار، نسبة الأجار
+
+281
+00:21:33,050 --> 00:21:36,230
+Zero، من الأمثلة عليها لـ Nutrient broth، أو
+
+282
+00:21:36,230 --> 00:21:39,650
+النيترات البروث، أو الـ brain heart infusion broth
+
+283
+00:21:40,640 --> 00:21:43,900
+ثاني معيار حسب الـ Nutrition، حسب المواد الغذائية،
+
+284
+00:21:43,900 --> 00:21:48,780
+بنصنفها إما لـ Simple أو Complex، الـ Simple بتحتوي
+
+285
+00:21:48,780 --> 00:21:53,300
+على مغذي واحد فقط، مصدر واحد للتغذية، من الأمثلة
+
+286
+00:21:53,300 --> 00:21:57,040
+عليها الـ Nutrient Water Broth، في هذه الميديا الـ
+
+287
+00:21:57,040 --> 00:22:01,400
+Nutrient هو مصدر التغذية الوحيد، الـ Complex بيكون فيه
+
+288
+00:22:01,400 --> 00:22:05,120
+أكثر من مصدر للتغذية، من الأمثلة عليها الـ Blood
+
+289
+00:22:05,120 --> 00:22:09,570
+Agar، ثالث معيار حسب الـ Function، تقسم إلى
+
+290
+00:22:09,570 --> 00:22:12,870
+General، Selective، Differential، Transport،
+
+291
+00:22:12,870 --> 00:22:17,710
+Enrichment، General، من اسمها General، بتنمي معظم
+
+292
+00:22:17,710 --> 00:22:20,710
+أنواع البكتيريا، Nutrient agar من الأمثلة عليها،
+
+293
+00:22:20,710 --> 00:22:25,950
+Selective، اختيارية، بتنمي أنواع معينة من البكتيريا،
+
+294
+00:22:25,950 --> 00:22:30,690
+متشابهة في  الخاصية، يعني حسب خاصية معينة، من الأمثلة
+
+295
+00:22:30,690 --> 00:22:35,340
+عليها الـ Mannitol Salt Agar، MSA، الـ MSA
+
+296
+00:22:35,340 --> 00:22:38,760
+بتنمي فقط البكتيريا اللي بتتحمل درجة الملوحة
+
+297
+00:22:38,760 --> 00:22:41,980
+العالية، الـ high concentration of salts، يبقى
+
+298
+00:22:41,980 --> 00:22:47,000
+الخاصية هي تحمل درجة الملوحة، الـ NaCl الموجود في
+
+299
+00:22:47,000 --> 00:22:53,140
+الـ media تركيزه 7.5%، أكيد بقى البكتيريا ما رح
+
+300
+00:22:53,140 --> 00:22:56,700
+تتحمل هذه الدرجة العالية من الـ salt، فبالتالي ما رح
+
+301
+00:22:56,700 --> 00:23:01,440
+تنمو، الـ differential، من اسمها differential، هتميز
+
+302
+00:23:01,440 --> 00:23:07,080
+أكثر من نوع من الـ Bacteria، بتعطي كل نوع لون معين،
+
+303
+00:23:07,080 --> 00:23:10,060
+من الأمثلة عليها الـ MacConkey agar، الـ
+
+304
+00:23:10,060 --> 00:23:12,940
+MacConkey agar تعتبر selective and
+
+305
+00:23:12,940 --> 00:23:17,300
+differential، الـ Transport media، الـ Transport media
+
+306
+00:23:17,300 --> 00:23:21,220
+من الأمثلة عليها الـ Stuart، Stuart transport
+
+307
+00:23:21,220 --> 00:23:27,200
+media، هو عبارة عن وسط فيه media، هي تستخدم لو ما
+
+308
+00:23:27,200 --> 00:23:31,000
+كان ضمن العين بعيد عن المختبر، أكيد بستخدم الـ
+
+309
+00:23:31,000 --> 00:23:35,730
+transport media، تحفظ البكتيريا عشان ما تموت خلال
+
+310
+00:23:35,730 --> 00:23:39,190
+عملية النقل قبل ما أنا أشتغل عليها، لو أنا ما عندي
+
+311
+00:23:39,190 --> 00:23:42,550
+Transport Media، ممكن أني أحطها في Normal Saline،
+
+312
+00:23:42,550 --> 00:23:46,650
+الـ Normal Saline هيحفظ البكتيريا من إنها تموت،
+
+313
+00:23:46,650 --> 00:23:51,430
+خامس نوع اللي هو الـ Enrichment، الـ Enrichment في أنها
+
+314
+00:23:51,430 --> 00:23:55,950
+تحتاج لبعض الإضافات بعد ما يتم تحضير الـ Media، لأن
+
+315
+00:23:55,950 --> 00:24:00,370
+في بعض أنواع البكتيريا تعتبر fastidious، مدلة،  لأنها
+
+316
+00:24:00,370 --> 00:24:06,940
+عشان تنمو، تحتاج إلى بعض الإضافات، من الإضافات ممكن
+
+317
+00:24:06,940 --> 00:24:11,940
+نضيف blood، ممكن نضيف egg، ممكن نضيف serum، بالنسبة
+
+318
+00:24:11,940 --> 00:24:16,200
+لأمثلة على الـ enriched، الـ Blood agar، أو الـ
+
+319
+00:24:16,200 --> 00:24:20,940
+Chocolate agar، بالنسبة للـ Blood agar، بنضيف blood، لكن
+
+320
+00:24:20,940 --> 00:24:24,260
+الـ blood بعد ما أنا أطلعه طبعا، هي يكون بعد الـ
+
+321
+00:24:24,260 --> 00:24:28,140
+Nutrient agar، بعد ما أنا أطلعه من الـ autoclave،
+
+322
+00:24:28,140 --> 00:24:33,700
+بخليه يبرد، بضيف الـ blood بعد ما يبرد، بنسبة خمسة في
+
+323
+00:24:33,700 --> 00:24:39,900
+المئة، يعني 5%،  لـ 100 مل من الـ Nutrient agar، لو
+
+324
+00:24:39,900 --> 00:24:44,460
+أنا حضرت ألف ملي من الـ Nutrient agar، بحط خمسين مل
+
+325
+00:24:44,460 --> 00:24:49,360
+من الـ blood على الألف مل من الـ Nutrient agar، لكن
+
+326
+00:24:49,360 --> 00:24:54,720
+للـ Chocolate agar، أنا بضيف الـ blood وهو ساخن، بعد ما أطلعه
+
+327
+00:24:54,720 --> 00:24:58,140
+الـ media من الـ autoclave وهي سخنة، ليش؟ لأن درجة
+
+328
+00:24:58,140 --> 00:25:02,200
+الحرارة العالية بتعمل denaturation للـ RBCs، بتطلع
+
+329
+00:25:02,200 --> 00:25:08,020
+V-factors، X-factors مهمين لنمو بعض أنواع
+
+330
+00:25:08,020 --> 00:25:12,240
+البكتيريا اللي هي fastidious، البكتيريا الـ Enrichment،
+
+331
+00:25:12,240 --> 00:25:17,060
+الـ Enrichment هي بتعمل inhibition لنمو الـ unwanted
+
+332
+00:25:17,060 --> 00:25:21,520
+بكتيريا، بالتالي بتسمح لنمو فقط الـ wanted بكتيريا،
+
+333
+00:25:21,520 --> 00:25:25,240
+أنها تنمو بكل أريحية، مثل الأمثلة عليها Selenite
+
+334
+00:25:25,240 --> 00:25:29,670
+F broth، بتنمي الـ Salmonella، الـ Alkaline peptone
+
+335
+00:25:29,670 --> 00:25:36,950
+water اللي بتنمي الـ Vibrio cholera، هاي
+
+336
+00:25:36,950 --> 00:25:41,870
+هي، الـ Transport media هي،  بتيجي بـ containers بتكون معقمة،
+
+337
+00:25:41,870 --> 00:25:46,570
+sterile، لو أنا بدي أخد مسحة، باخد المسحة بالسواب،
+
+338
+00:25:46,570 --> 00:25:51,210
+هذا، بعد ما أخد المسحة، بحطه في التيوب، هذا اللي
+
+339
+00:25:51,210 --> 00:25:52,870
+موجود في الميديا،
+
+340
+00:25:57,380 --> 00:26:01,020
+Forms of Culture Media، أشكال الـ Culture Media، أول
+
+341
+00:26:01,020 --> 00:26:05,580
+شكل، broth، الـ liquid media، ممكن تكون broth طبعا،
+
+342
+00:26:05,580 --> 00:26:10,280
+البروث بحطها بـ test tubes containing a liquid media،
+
+343
+00:26:10,280 --> 00:26:14,700
+أنابيب تحتوي على liquid media، على ميديا سائلة، a
+
+344
+00:26:14,700 --> 00:26:19,580
+typical nutrient containing broth، ميديا سائلة، broth
+
+345
+00:26:19,580 --> 00:26:25,370
+Nutrient broth، Thioglycollate broth، من الأمثلة على البروث، طيب بعد
+
+346
+00:26:25,370 --> 00:26:28,870
+ما أزرع البكتيريا في broth، كيف أعرف نمو
+
+347
+00:26:28,870 --> 00:26:33,330
+البكتيريا أو لأ؟ اللي بتلاقيه، فيه ثلاث أشكال،
+
+348
+00:26:33,330 --> 00:26:37,930
+ممكن تلاحظيه، أحيانًا ممكن تلاقيه كـ pellicle، أو a
+
+349
+00:26:37,930 --> 00:26:41,690
+combination of three forms، أو تلاقيه معكر،
+
+350
+00:26:41,690 --> 00:26:48,310
+الثلاث أشكال، نتعرف على الثلاث أشكال، أول واحد الـ pellicle،
+
+351
+00:26:48,310 --> 00:26:52,170
+a mass of organisms is floating on top of the
+
+352
+00:26:52,170 --> 00:26:57,500
+broth، بقول لي بتكون البكتيريا بتنمو على السطح،
+
+353
+00:26:57,500 --> 00:27:02,620
+بتطفو على السطح، turbidity، the organism appear as a
+
+354
+00:27:02,620 --> 00:27:06,100
+general cloudiness throughout the broth، بتكون
+
+355
+00:27:06,100 --> 00:27:11,360
+معكرة،  entire tube، sediment، a mass of organisms، كتلة من
+
+356
+00:27:11,360 --> 00:27:15,480
+الخلايا بتكون at the bottom of the tube، بتكون
+
+357
+00:27:15,480 --> 00:27:20,870
+مترسبة في القاع، طبعا الاختلاف حسب نوع البكتيريا،
+
+358
+00:27:20,870 --> 00:27:25,330
+واختلاف احتياجاتها للأكسجين، فمثلًا الـ pellicle
+
+359
+00:27:25,330 --> 00:27:29,950
+بتكون aerobic، لكن الـ sediment بتكون anaerobic،
+
+360
+00:27:29,950 --> 00:27:35,730
+طريقة الزراعة، باخد الـ inoculum أو البكتيريا، وبعمل
+
+361
+00:27:35,730 --> 00:27:37,350
+mix،  لبروث
+
+362
+00:27:42,170 --> 00:27:46,390
+بالنسبة لأشكال النمو في الـ broth tube، لو كانت
+
+363
+00:27:46,390 --> 00:27:50,510
+مجموعة الخلايا مترسبة في القاع، يبقى بتكون sediment،
+
+364
+00:27:50,510 --> 00:27:54,270
+لو كانت طافية على السطح pellicle، بنلاحظ بنفس الـ
+
+365
+00:27:54,270 --> 00:27:58,650
+tube، في عندي كمان مجموعة من الـ organisms مترسبة في
+
+366
+00:27:58,650 --> 00:28:01,650
+القاع، يبقى sediment، في هاي الـ tube في عندي
+
+367
+00:28:01,650 --> 00:28:05,010
+pellicle and sediment، في الـ slide قبل قلنا ممكن
+
+368
+00:28:05,010 --> 00:28:09,910
+ألاقي نمو بشكل واحد أو بأكثر من شكل،  أخر شكل للـ broth tube
+
+369
+00:28:09,910 --> 00:28:14,230
+tube معكر، خلّصنا الـ broth tube أو الشكل ده من
+
+370
+00:28:14,230 --> 00:28:18,110
+أشكال الـ culture media، ثانيًا، شكل الـ slant tube،
+
+371
+00:28:18,110 --> 00:28:22,310
+are tubes containing a nutrient medium،  بصلب
+
+372
+00:28:22,310 --> 00:28:27,470
+صلب،  بـ agar، أنابيب تحتوي على الـ nutrient media
+
+373
+00:28:27,470 --> 00:28:33,130
+على وسط غذائي مع صلب agar، في الـ broth tube كان
+
+374
+00:28:33,130 --> 00:28:37,070
+في عندي وسط غذائي لكن ما كان في عندي صلب agar،
+
+375
+00:28:37,070 --> 00:28:40,750
+ما كان في عندي agar، كانت فيه broth سائل، هذا الفرق
+
+376
+00:28:40,750 --> 00:28:47,390
+بينها وبين، بالنسبة للإسلانت tube، كيف احصل على شكل
+
+377
+00:28:47,390 --> 00:28:55,190
+الإسلانت؟ شكل الإسلانت بيكون هكذا، لو رسمناه، هي الـ tube
+
+378
+00:28:55,190 --> 00:29:02,690
+بيكون مائل، كيف احصل على شكل الإسلانت؟ بتركها تتصلب
+
+379
+00:29:02,690 --> 00:29:05,630
+في زاوية، يعني بعد ما أطلعها من الـ autoclave، بحطها
+
+380
+00:29:05,630 --> 00:29:09,550
+في زاوية عشان تتصلب في زاوية، كيف بزروع الـ slant tube؟
+
+381
+00:29:09,550 --> 00:29:14,870
+بدخل بعمل طعن للمنطقة اللي تحت، بدخل بالـ
+
+382
+00:29:14,870 --> 00:29:18,750
+inoculating needle، بعمل طعن للمنطقة اللي تحت، بعدين
+
+383
+00:29:18,750 --> 00:29:22,630
+بطلع، بعمل zigzag للمنطقة المائلة، هذا بيكون السطح،
+
+384
+00:29:22,630 --> 00:29:27,550
+بعمل zigzag عشان أزرع على هذا السطح البكتيريا، طب
+
+385
+00:29:27,550 --> 00:29:32,030
+ليش بزرع البكتيريا بطريقة الـ streak plate؟ in order
+
+386
+00:29:32,030 --> 00:29:36,510
+to get a flat inoculating surface، it is used for
+
+387
+00:29:36,510 --> 00:29:40,840
+transport and storage، هذه الحالة بيكون عندي مساحة
+
+388
+00:29:40,840 --> 00:29:44,900
+سطح أكبر، يبقى بحصل على كمية أكبر من البكتيريا،
+
+389
+00:29:44,900 --> 00:29:49,660
+بيكون مناسب في حالة الـ transport، في حالة النقل أو
+
+390
+00:29:49,660 --> 00:29:52,860
+الـ storage، التخزين، لو بدي أخزن البكتيريا، الـ
+
+391
+00:29:52,860 --> 00:29:57,340
+slant tube مناسبة لأنها بتوفر مساحة سطح أكبر،
+
+392
+00:29:57,340 --> 00:30:01,760
+ثالث شكل من أشكال الـ cultural media، للـ stab culture،
+
+393
+00:30:01,760 --> 00:30:05,840
+نفس الـ slant tube، أنابيب تحتوي على وسط غذائي
+
+394
+00:30:05,840 --> 
+
+445
+00:34:01,710 --> 00:34:05,550
+Aseptic technique يعني كل الإجراءات التي يتم
+
+446
+00:34:05,550 --> 00:34:09,070
+اتخاذها لمنع الـ contamination.  the most commonly
+
+447
+00:34:09,070 --> 00:34:12,590
+used device for moving bacteria is the inoculating
+
+448
+00:34:12,590 --> 00:34:16,830
+loop. بقول لي إن الـ inoculating loop هي أداة استخدام
+
+449
+00:34:16,830 --> 00:34:21,210
+لنقل بكتيريا من وسط لآخر وزراعتها.  The inoculating
+
+450
+00:34:21,210 --> 00:34:24,610
+loop ممكن أن تكون من النيكروم (Nichrome) and alloy
+
+451
+00:34:24,610 --> 00:34:28,730
+هي سبيكة of nickel and chromium، أو ممكن تكون من
+
+452
+00:34:28,730 --> 00:34:33,170
+البلاتينيوم wire. بالنسبة للـ inoculating loop
+
+453
+00:34:33,170 --> 00:34:37,230
+بنلاحظ أن في عندي أنا حلقة (loop) at one end، في
+
+454
+00:34:37,230 --> 00:34:40,610
+نهايتها.  في حلقة. الحلقة هي اللي بأخذ البكتيريا
+
+455
+00:34:40,610 --> 00:34:44,890
+فيها، والجزء الثاني اللي هو أنا بمسكه. وهذا بالنسبة
+
+456
+00:34:44,890 --> 00:34:48,790
+للـ inoculating loop. في أداة تشبهها اسمها الـ
+
+457
+00:34:48,790 --> 00:34:52,050
+inoculating needle اللي احنا استخدمناها في الـ
+
+458
+00:34:52,050 --> 00:34:55,850
+motility. هي نفس الشيء لكن مش موجود في handy loop.
+
+459
+00:34:55,850 --> 00:35:04,110
+ما في handy loop. هي straight wire. كيف
+
+460
+00:35:04,110 --> 00:35:07,350
+أنا بدي أعقم الـ inoculating loop أو الـ needle؟
+
+461
+00:35:07,350 --> 00:35:10,750
+باستخدام Bunsen burner.  بنحافظ على جزء الـ wire في
+
+462
+00:35:10,750 --> 00:35:15,110
+اللهب حتى يتوهج.  بنعقم الأداتين
+
+463
+00:35:15,110 --> 00:35:20,820
+بتعريضهم للهب لما يتوهج (glow) يصير لونه أحمر، هيك
+
+464
+00:35:20,820 --> 00:35:24,680
+بيكون تم تعقيمه. The instrument should be allowed
+
+465
+00:35:24,680 --> 00:35:29,060
+to cool. بقولي بعد ما تم عملية التعقيم، بستنى غلق
+
+466
+00:35:29,060 --> 00:35:33,240
+اللوب أو الـ needle إنها تبرد لمدة عشر إلى عشرين
+
+467
+00:35:33,240 --> 00:35:37,660
+second قبل ما أنا أستخدمها، why? To avoid killing
+
+468
+00:35:37,660 --> 00:35:41,920
+the inoculum. عشان ما أقتل الـ inoculum.  الـ inoculum
+
+469
+00:35:41,920 --> 00:35:45,290
+المزرعة اللي أنا أخدتها. In this way, all contaminants
+
+470
+00:35:45,290 --> 00:35:49,250
+on the wire are incinerated. في هذه الطريقة كل الـ
+
+471
+00:35:49,250 --> 00:35:52,990
+contaminants اللي على الـ wire أو على الـ loop
+
+472
+00:35:52,990 --> 00:35:57,470
+بيصيروا incinerated. ملاحظات: Don't blow on the
+
+473
+00:35:57,470 --> 00:36:00,950
+instrument to cool them. ممنوع أني أنفخ على الـ loop
+
+474
+00:36:00,950 --> 00:36:05,350
+أو الـ needle عشان أخليه يبرد. ممنوع Don't touch the
+
+475
+00:36:05,350 --> 00:36:09,090
+instrument to the agar to cool them. ممنوع أعمل touch
+
+476
+00:36:09,090 --> 00:36:12,850
+بالأجار عشان أخليه يبرد. Don't lay the loop down
+
+477
+00:36:12,850 --> 00:36:17,170
+once it is sterilized. ممنوع أخلي اللوب أو الـ
+
+478
+00:36:17,170 --> 00:36:20,470
+needle بعد ما أعقمه أحطه على الـ bench، لأنه بيصير
+
+479
+00:36:20,470 --> 00:36:27,010
+contaminated. Procedure
+
+480
+00:36:27,010 --> 00:36:31,050
+for aseptically transferring: أول شي بدي أعقم اللوب
+
+481
+00:36:31,420 --> 00:36:34,220
+والـ needle. ثاني شيء: بدون ما أحط اللوب على الـ bench
+
+482
+00:36:34,220 --> 00:36:38,160
+بمسك الـ culture tube، بعقم الفوهة بتمريرها على
+
+483
+00:36:38,160 --> 00:36:42,260
+اللّهب بسرعة. ممنوع أحط الغطاية على الـ bench. بضّل
+
+484
+00:36:42,260 --> 00:36:46,500
+مسكاها بإيدي. الغطاية بمسكها بنفس الإيد اللي ماسكة
+
+485
+00:36:46,500 --> 00:36:52,460
+فيها اللوب. ثالث شيء: بدخل اللوب في الـ tube وبزرع.
+
+486
+00:36:52,460 --> 00:36:56,700
+رابع شيء: بعقم، بعد ما أخلص الشغل، بعقم الفوهة و
+
+487
+00:36:56,700 --> 00:37:01,620
+برجعها. خامس شيء: بكرّر نفس الخطوات في حال أنا زرعت
+
+488
+00:37:01,620 --> 00:37:08,780
+أكثر من الـ tube. لما
+
+489
+00:37:08,780 --> 00:37:13,340
+أخلص الشغل بعقم اللوب وبحطه على الـ bench. لو أنا
+
+490
+00:37:13,340 --> 00:37:19,720
+نسيت إني أنا ما عقمت، بعقم هانِ
+
+491
+00:37:19,720 --> 00:37:24,400
+الـ loop. الخطوات في صور: بعقم الـ loop بتعريضه للـلّهب لما
+
+492
+00:37:24,400 --> 00:37:28,850
+يصير لونه أحمر، يتوهج، وعرفت تم تعقيمه.  بخليه يبرد
+
+493
+00:37:28,850 --> 00:37:34,870
+قبل ما أستخدمه من 10 لـ 20 second عشان أمنع الـ
+
+494
+00:37:34,870 --> 00:37:40,830
+killing لل inoculum. بمسك
+
+495
+00:37:40,830 --> 00:37:45,630
+الـ culture tube، برفع الغطاية، بعقم الفوهة. ممنوع أحط
+
+496
+00:37:45,630 --> 00:37:50,410
+الغطاية على الـ bench. بزرع. بعد ما أخلص الشغل بعقم
+
+497
+00:37:50,410 --> 00:37:52,830
+الفوهة وبرجع الغطاية.
+
+498
+00:37:58,120 --> 00:38:02,160
+الجزء اللي هبوط أول جزء بيكون dark zone of
+
+499
+00:38:02,160 --> 00:38:06,460
+unburned gas. هذه المنطقة ما بيصير فيها احتراق.
+
+500
+00:38:06,460 --> 00:38:10,060
+بعدين عندي الـ orange zone of incomplete combustion.
+
+501
+00:38:10,060 --> 00:38:13,600
+بعدين بتيجي منطقة الـ blue zone of partial
+
+502
+00:38:13,600 --> 00:38:17,400
+combustion. أخر شيء الـ colorless zone of complete
+
+503
+00:38:17,400 --> 00:38:21,860
+combustion. كل ما طلعنا لفوق بيزيد الـ oxygen، بيصير
+
+504
+00:38:21,860 --> 00:38:26,400
+sufficient combustion، احتراق كامل للغاز، وبالتالي
+
+505
+00:38:26,400 --> 00:38:30,260
+بتكون شديدة الحرارة (hottest). يبقى اللي بدنا نعرفه
+
+506
+00:38:30,260 --> 00:38:34,820
+أن التعقيم بيحصل عند المنطقة الزرقاء أو المنطقة الـ
+
+507
+00:38:34,820 --> 00:38:40,520
+colorless.  نسميها non-luminous flame، بتكون hottest
+
+508
+00:38:40,520 --> 00:38:45,400
+بتكون شديدة الحرارة. لكن الـ luminous flame بتكون هي
+
+509
+00:38:45,400 --> 00:38:50,660
+المنطقة الـ orange، not very hot. ماذا نستخدمها في
+
+510
+00:38:50,660 --> 00:38:55,180
+تعقيم اللوب؟ نستخدم في تعقيم اللوب، تعقيم عند
+
+511
+00:38:55,180 --> 00:39:00,100
+المنطقة الزرقاء، الـ blue، أو الـ colorless، لأنه بتكون
+
+512
+00:39:00,100 --> 00:39:04,920
+hottest. Five
+
+513
+00:39:04,920 --> 00:39:10,100
+basic techniques of culturing: إنكولات فتحت tube
+
+514
+00:39:10,100 --> 00:39:14,000
+أو plate، وأخدت inoculum وزرعت. بعد ما زرعت دخلت
+
+515
+00:39:14,000 --> 00:39:17,040
+الـ plate على الـ incubator وعملت incubation. يبقى
+
+516
+00:39:17,040 --> 00:39:21,620
+المرحلة الثانية incubation. مدة 24 ساعة وأحياناً 48
+
+517
+00:39:21,620 --> 00:39:25,640
+حسب البكتيريا. بعد الـ incubation هلاقي نمو
+
+518
+00:39:25,640 --> 00:39:29,660
+للبكتيريا. ممكن ينمو أكثر من نوع من البكتيريا. بأخذ
+
+519
+00:39:29,660 --> 00:39:32,920
+كل نوع وبزرعه على plate.  هاي المرحلة بسميها
+
+520
+00:39:32,920 --> 00:39:37,360
+isolation. يبقى المرحلة الثانية الـ isolation. بعدها
+
+521
+00:39:37,360 --> 00:39:41,480
+هأعمل اختبارات تعريفية في مرحلة الـ examination عشان
+
+522
+00:39:41,480 --> 00:39:45,240
+أعمل identification للبكتيريا اللي هي المرحلة
+
+523
+00:39:45,240 --> 00:39:45,940
+الأخيرة.
+
+524
+00:39:49,790 --> 00:39:53,370
+Pure culture concept: To identify bacteria in a
+
+525
+00:39:53,370 --> 00:39:56,670
+clinical sample cannot be done unless isolated
+
+526
+00:39:56,670 --> 00:40:00,290
+colonies are used. عشان أتعرف على البكتيريا لازم يكون
+
+527
+00:40:00,290 --> 00:40:04,490
+عندي isolated colony. الـ isolated colony أصلها خلية
+
+528
+00:40:04,490 --> 00:40:09,270
+بكتيرية واحدة. كيف بدي أحصل على الـ isolated colony؟
+
+529
+00:40:09,270 --> 00:40:13,190
+To obtain well-isolated colonies, it is essential to
+
+530
+00:40:13,190 --> 00:40:17,270
+disperse the inoculum or sample on the surface of
+
+531
+00:40:17,270 --> 00:40:21,570
+an enriched agar plate. عشان أحصل على isolated
+
+532
+00:40:21,570 --> 00:40:26,110
+colonies، مهم جداً أوزع العينة على سطح الـ plate،  بأربعة أرباع.
+
+533
+00:40:26,110 --> 00:40:29,830
+So the individual bacteria are well separated from
+
+534
+00:40:29,830 --> 00:40:34,230
+each other. هنقدر نفصل البكتيريا عن بعض بطريقة
+
+535
+00:40:34,230 --> 00:40:39,810
+الأربعة أرباع. هنخفف، وبالتالي هنفصل المستعمرات عن بعض، هيك
+
+536
+00:40:39,810 --> 00:40:44,790
+بضمن أن الـ isolated colonies أصلها خلية بكتيرية واحدة.
+
+537
+00:40:45,190 --> 00:40:48,610
+لكن لو أنا أخدت من الربع الأول، الربع الأول بيكون
+
+538
+00:40:48,610 --> 00:40:52,850
+فيه المستعمرات فوق بعض، ممكن تكون بكتيريا X فوق
+
+539
+00:40:52,850 --> 00:40:56,790
+بكتيريا Y، يعني بيكون عندي mixed population.  روحت
+
+540
+00:40:56,790 --> 00:41:00,910
+أنا اشتغلت تحاليل، هيكون كل نتائج التحاليل غلط، لأنه
+
+541
+00:41:00,910 --> 00:41:04,630
+اشتغلت على بكتيريا X وبكتيريا Y. لازم فقط أنا
+
+542
+00:41:04,630 --> 00:41:08,650
+أشتغل على بكتيريا واحدة في الـ isolated colony.
+
+543
+00:41:08,650 --> 00:41:14,740
+بتكون المستعمرات بعيدة عن بعض، بضمن أن أصلها بكتيريا
+
+544
+00:41:14,740 --> 00:41:19,100
+واحدة.
+
+545
+00:41:19,100 --> 00:41:25,660
+Contaminants:
+
+546
+00:41:25,660 --> 00:41:29,480
+other microorganisms present in the sample. كائنات
+
+547
+00:41:29,480 --> 00:41:33,500
+أخرى موجودة في العينة أو في المزرعة.  فمثلاً،
+
+548
+00:41:33,500 --> 00:41:37,990
+إذا زرعت يورين وزرعتها، لكن ما عقمت الـ loop، هينمو
+
+549
+00:41:37,990 --> 00:41:41,830
+microorganisms على الوسط غير الموجود في عينة الـ
+
+550
+00:41:41,830 --> 00:41:46,390
+urine، نتيجة عدم تعقيم الـ loop. Isolated colonies:
+
+551
+00:41:46,390 --> 00:41:49,390
+a population of millions of cells that are
+
+552
+00:41:49,390 --> 00:41:53,970
+identical and are descended from a single founder
+
+553
+00:41:53,970 --> 00:41:57,690
+cell. الـ isolated colony لو أنا زرعت في طريقة
+
+554
+00:41:57,690 --> 00:42:01,130
+الأربعة أرباع، هتكون موجودة في الربع الأخير، هي ملايين
+
+555
+00:42:01,130 --> 00:42:05,970
+الخلايا المتشابهة اللي أصلها خلية بكتيرية واحدة. لو
+
+556
+00:42:05,970 --> 00:42:09,150
+أخدتها وروح زرعتها على طبق، بسميه طبق pure
+
+557
+00:42:09,150 --> 00:42:13,490
+culture. هيك بيصير كل الطبق من بكتيريا واحدة. بلاحظ
+
+558
+00:42:13,490 --> 00:42:18,690
+إن كل الـ colonies نفس المورفولوجي. Stock culture،
+
+559
+00:42:18,690 --> 00:42:21,570
+stock يعني مخزون، a culture that already contains
+
+560
+00:42:21,570 --> 00:42:26,570
+cells. It is used as a source of cells from which to
+
+561
+00:42:26,570 --> 00:42:31,450
+manipulate new cultures. تحتوي على خلايا تستخدم كمصدر
+
+562
+00:42:31,450 --> 00:42:35,310
+للخلايا البكتيرية عشان تزرع مرة ثانية. الأطباق اللي
+
+563
+00:42:35,310 --> 00:42:38,990
+بتزرع منها وبتأخذ منها بكتيريا تعتبر stock culture
+
+564
+00:42:38,990 --> 00:42:43,930
+عشان تعملوا a new culture. الـ culture media ممكن
+
+565
+00:42:43,930 --> 00:42:48,750
+تكون rich، غنية بمواد غذائية بتنمي كل الأنواع، or
+
+566
+00:42:48,750 --> 00:42:52,590
+selective، بتنمي نوع معين. الـ growth فيها مبادئ
+
+567
+00:42:52,590 --> 00:42:57,100
+لنمو البكتيريا، or inhibitors، فيها مثبطات. ممكن تكون
+
+568
+00:42:57,100 --> 00:43:01,580
+liquid أو solid. الـ temperature لازم تكون مناسبة
+
+569
+00:43:01,580 --> 00:43:06,000
+لنمو البكتيريا. Source of energy: مفضلة الطاقة زي
+
+570
+00:43:06,000 --> 00:43:10,280
+الجلوكوز، اللاكتوز، المانيتول. Source of carbon أو
+
+571
+00:43:10,280 --> 00:43:14,240
+nitrogen. أكيد لازم يكون في عندي carbon source أو
+
+572
+00:43:14,240 --> 00:43:18,060
+nitrogen source عشان تنمو البكتيريا وتتكاثر. من
+
+573
+00:43:18,060 --> 00:43:22,700
+أمثلة على carbon source: الكربوهيدرات. الـ nitrogen
+
+574
+00:43:22,700 --> 00:43:26,810
+source: الأحماض الأمينية. Aseptic techniques include
+
+575
+00:43:26,810 --> 00:43:30,090
+sterilization of media. لازم إني أنا أعقم الـ media.
+
+576
+00:43:30,090 --> 00:43:34,870
+Sterilization of equipment. لازم إني أنا أعقم الأدوات.
+
+577
+00:43:34,870 --> 00:43:40,050
+Proper handling: إني أنا أشتغل مثلاً جنب اللهب. ممنوع
+
+578
+00:43:40,050 --> 00:43:43,350
+إني أنا أنفخ عشان يبرد الـ loop. أتعقم الـ bench قبل
+
+579
+00:43:43,350 --> 00:43:47,410
+الشغل، وبعد هذا كله تعتبر aseptic technique عشان
+
+580
+00:43:47,410 --> 00:43:48,710
+أمنع الـ contamination.
+
+581
+00:43:55,320 --> 00:43:59,720
+الأدوات اللي هستخدمها في الشغل: Inoculating loops،
+
+582
+00:43:59,720 --> 00:44:04,580
+Bunsen burner للتعقيم. Stock culture. قلنا إن stock culture
+
+583
+00:44:04,580 --> 00:44:08,320
+هي مخزون تحتوي على خلايا عشان أنا أعمل a new
+
+584
+00:44:08,320 --> 00:44:12,540
+culture. أكيد agar plates اللي أنا بدي أزرع
+
+585
+00:44:12,540 --> 00:44:12,840
+عليها.
+
+586
+00:44:20,110 --> 00:44:24,510
+كيف طريقة الشغل عشان أحصل على isolated colonies؟ أول
+
+587
+00:44:24,510 --> 00:44:29,770
+خطوة: بعقم اللوب بالـ Bunsen burner، بخليه يبرد. بأخذ inoculum من الـ
+
+588
+00:44:29,770 --> 00:44:35,050
+stock culture، وأكيد بعقم الفوهة. بفرض الـ inoculum على
+
+589
+00:44:35,050 --> 00:44:38,990
+الربع الأول، بحاول يكون continuous، قريب من بعض.
+
+590
+00:44:38,990 --> 00:44:42,370
+ثاني خطوة: بتوقع أنا في الربع الأول مش هيكون
+
+591
+00:44:42,370 --> 00:44:46,270
+isolated colonies. بحاول إني أنا ما أضغط على الأجار
+
+592
+00:44:46,270 --> 00:44:50,850
+وأنا بوزع، أو أستخدم plastic loop، بتخلص منه. ثالث
+
+593
+00:44:50,850 --> 00:44:54,910
+خطوة: بقول لك اقلبي الطبق 90 درجة، وزرعي الربع
+
+594
+00:44:54,910 --> 00:44:59,070
+الثاني. خذي من الربع الأول، وعمل خطوط. ما ترجعي
+
+595
+00:44:59,070 --> 00:45:07,430
+تاخذي من الـ stock، لأن أنا بدي أحفظ. بكرر
+
+596
+00:45:07,430 --> 00:45:12,450
+نفس الخطوات في الربع الثالث وفي الربع الرابع.
+
+597
+00:45:12,450 --> 00:45:16,570
+بحاول إني أتعقم الـ loop بين ربع وربع. لو أنا
+
+598
+00:45:16,570 --> 00:45:20,820
+استخدمت plastic loop. إذا nichrome loop، بعقم الـ nichrome
+
+599
+00:45:20,820 --> 00:45:24,900
+loop. آخر شيء: بقول إني بحط الأطباق في الـ incubator
+
+600
+00:45:24,900 --> 00:45:29,240
+upside-down. Why? To prevent condensation. حتى أمنع
+
+601
+00:45:29,240 --> 00:45:34,200
+التكثيف فيها. الملاحظة: إنه أنا لما أفتح tube، بحاول
+
+602
+00:45:34,200 --> 00:45:37,580
+إني أنا ما أمسكها من الـ lid، لأن ممكن أنها تكون
+
+603
+00:45:37,580 --> 00:45:45,500
+مفتوحة وتنكسر.  هذه
+
+604
+00:45:45,500 --> 00:45:50,590
+الخطوات.  في سؤال: أول شيء بعقم الـ loop، بأخذ inoculum،
+
+605
+00:45:50,590 --> 00:45:54,250
+وبزرع الربع الأول بشكل continuous، بكون قريب من
+
+606
+00:45:54,250 --> 00:46:00,410
+بعض. بعدين بعقم الـ loop، بقلب الـ plate 90 درجة، بأخذ من
+
+607
+00:46:00,410 --> 00:46:05,670
+الربع الأول وبطلع. بعدها بقلب 90 درجة، وطبعاً بعقم
+
+608
+00:46:05,670 --> 00:46:11,290
+الـ loop، بأخذ من الربع الثاني وبطلع. بقلب 90 درجة و
+
+609
+00:46:11,290 --> 00:46:15,810
+أكيد بعقم الـ loop، بأخذ من الربع الثالث، بعمل deep
+
+610
+00:46:15,810 --> 00:46:16,210
+streak.
+
+611
+00:46:20,320 --> 00:46:24,160
+في عندي ثلاث طرق لزراعة الأجار plates. أول طريقة
+
+612
+00:46:24,160 --> 00:46:26,400
+تعرفنا عليها بالـ slide اللي قبل، اللي هي الـ
+
+613
+00:46:26,400 --> 00:46:30,480
+quadrant streak. هاي الطريقة بستخدمها للحصول على
+
+614
+00:46:30,480 --> 00:46:34,360
+isolated colonies، لعمل pure culture، عشان أتعرف على
+
+615
+00:46:34,360 --> 00:46:38,760
+البكتيريا. الطريقة الثانية: الـ radiant streak، اللي
+
+616
+00:46:38,760 --> 00:46:43,860
+هي الشعاعي. بستخدمها حتى أحصل أيضاً على isolated
+
+617
+00:46:43,860 --> 00:46:47,680
+colonies.  في طريقة الزراعة: بزرع الربع الأول بشكل
+
+618
+00:46:47,680 --> 00:46:53,820
+continuous. بعدين بطلع شعاع من ال
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ca4bc2mptiY.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ca4bc2mptiY.srt
new file mode 100644
index 0000000000000000000000000000000000000000..b3b0f6b9a21ac7c094e2037d57c1149e7013895f
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ca4bc2mptiY.srt
@@ -0,0 +1,1855 @@
+
+1
+00:00:02,140 --> 00:00:06,040
+السلام عليكم ورحمة الله وبركاته في هذا اللقاء
+
+2
+00:00:06,040 --> 00:00:10,080
+هتكون المحاضرة بعنوان Citrate Utilization and
+
+3
+00:00:10,080 --> 00:00:14,940
+Oxidase Production. هنتكلم عن إنزيم الـ Citrate وعن
+
+4
+00:00:14,940 --> 00:00:20,140
+إنزيم الـ Oxidase، وعرفنا إن هدول الإنزيمين يعتبروا
+
+5
+00:00:20,140 --> 00:00:24,920
+Endoenzyme. هنبدأ بـ Citrate Utilization، استهلاك
+
+6
+00:00:24,920 --> 00:00:28,380
+الـ Citrate. ذكرنا سابقًا أنواع الـ enzymatic
+
+7
+00:00:28,380 --> 00:00:32,390
+reactions، كان من ضمنها utilization of non-
+
+8
+00:00:32,390 --> 00:00:36,630
+carbohydrate carbon source. ذكرنا مثال اليوريا
+
+9
+00:00:36,630 --> 00:00:41,530
+والسيتريت. طيب، ليش البكتيريا بدها carbon source؟
+
+10
+00:00:41,530 --> 00:00:45,570
+بقول لي البكتيريا عشان تنمو وتتكاثر، بحاجة لـ DNA
+
+11
+00:00:45,570 --> 00:00:49,450
+replication، عشان يصير بعدها transcription، بعدين
+
+12
+00:00:49,450 --> 00:00:54,030
+يصير الـ translation لتكوين الـ protein، عشان يتكون
+
+13
+00:00:54,030 --> 00:00:58,280
+protein. فهي بحاجة لـ carbon and nitrogen source.
+
+14
+00:00:58,280 --> 00:01:03,660
+البكتيريا راح تستهلك مصادر للحصول عليهم. بالنسبة للـ
+
+15
+00:01:03,660 --> 00:01:07,540
+carbon source، ممكن إنّها تستهلك الكربوهيدرات، السكريات،
+
+16
+00:01:07,540 --> 00:01:12,280
+وممكن إنّها تستهلك من مصادر non-carbohydrate، زي
+
+17
+00:01:12,280 --> 00:01:16,960
+اليوريا، الـ amino acid، السيترات. اليوم موضوعنا هيكون
+
+18
+00:01:16,960 --> 00:01:22,140
+عن استهلاك السيترات، كيف البكتيريا بتستهلك السيترات
+
+19
+00:01:22,140 --> 00:01:26,550
+للحصول على الـ carbon source. بالنسبة للـ nitrogen
+
+20
+00:01:26,550 --> 00:01:29,590
+source، في عندي الـ amino acid، الأحماض الأمينية، هي تعتبر
+
+21
+00:01:29,590 --> 00:01:34,770
+nitrogen source، لازمة لنمو البكتيريا. السيترات
+
+22
+00:01:34,770 --> 00:01:38,630
+يبقى يعتبر non-carbohydrate carbon source، بيكون
+
+23
+00:01:38,630 --> 00:01:43,250
+على شكل sodium citrate. في بعض أنواع البكتيريا
+
+24
+00:01:43,250 --> 00:01:47,350
+قادرة على استهلاك الـ sodium citrate، والحصول على
+
+25
+00:01:47,350 --> 00:01:52,310
+carbon لازم لنمو البكتيريا، وانقسامها. لكن في بعض
+
+26
+00:01:52,310 --> 00:01:55,250
+أنواع البكتيريا ما عندها القدرة على استهلاك
+
+27
+00:01:55,250 --> 00:01:59,870
+السيترات. طب، كيف بتكون السيترات داخل الخلية؟ زي ما
+
+28
+00:01:59,870 --> 00:02:03,750
+قلنا، في الكتالاز، الـ hydrogen peroxide بتكون خلال
+
+29
+00:02:03,750 --> 00:02:09,170
+عملية التنفس الخلوي. السيترات لازم يكون intermediate
+
+30
+00:02:09,170 --> 00:02:14,550
+في إحدى العمليات الأيضية، عشان يصير استهلاكه. بقول لي
+
+31
+00:02:14,550 --> 00:02:17,870
+أنّ السيترات، الـ sodium citrate، بيكون intermediate
+
+32
+00:02:17,870 --> 00:02:22,970
+في الـ Krebs cycle. في الـ Krebs cycle، يبقى الـ sodium
+
+33
+00:02:22,970 --> 00:02:26,270
+citrate هو الـ substrate اللي هيشتغل عليه الـ enzyme
+
+34
+00:02:26,270 --> 00:02:30,910
+بيكون في الـ in vivo intermediate في الـ Krebs cycle.
+
+35
+00:02:30,910 --> 00:02:36,430
+بالنسبة للتفاعل، كيف هتستهلك البكتيريا الـ sodium
+
+36
+00:02:36,430 --> 00:02:40,630
+citrate؟ بقول لي أنّ إنزيمات السيترات تعتبر
+
+37
+00:02:40,630 --> 00:02:45,130
+endoenzyme. عشان البكتيريا تقدر تستهلك الـ sodium
+
+38
+00:02:45,130 --> 00:02:48,830
+citrate، لازم يكون عند البكتيريا إنزيم الـ citrate
+
+39
+00:02:48,830 --> 00:02:54,870
+lyase. لازم هذا الإنزيم يكسر sodium citrate اللي اتكون
+
+40
+00:02:54,870 --> 00:03:00,330
+في الـ Krebs cycle. هِيحول sodium citrate لـ
+
+41
+00:03:00,330 --> 00:03:05,100
+oxaloacetic acid زائد أسيتات. كمان البكتيريا اللي
+
+42
+00:03:05,100 --> 00:03:08,380
+عندها القدرة، أو اللي عندها إنزيم citrate lyase، في
+
+43
+00:03:08,380 --> 00:03:12,360
+عندها إنزيم الـ oxaloacetic acid decarboxylase،
+
+44
+00:03:12,360 --> 00:03:17,640
+هِتنزع الـ CO2، الكربون، من الـ oxaloacetic acid،
+
+45
+00:03:17,640 --> 00:03:22,060
+هيتكون عندي بيروفات. البيروفات هو الـ carbon source،
+
+46
+00:03:22,060 --> 00:03:27,500
+هو الـ carbon source اللي لازم لنمو البكتيريا،
+
+47
+00:03:27,500 --> 00:03:31,550
+وانقسامها. احنا قلنا إنّ الـ oxaloacetic acid هيعمل
+
+48
+00:03:31,550 --> 00:03:35,910
+decarboxylation، هينزع الـ CO2. في عندي الصوديوم،
+
+49
+00:03:35,910 --> 00:03:39,210
+الـ excess من الصوديوم مع الـ CO2 هيتكون
+
+50
+00:03:39,210 --> 00:03:45,910
+عندي Na2CO3. هذا يعتبر alkaline، فبالتالي هيرفع لي
+
+51
+00:03:45,910 --> 00:03:50,810
+الـ pH. يبقى صار عندي مصدر كربون من non-
+
+52
+00:03:50,810 --> 00:03:55,820
+carbohydrate carbon source من الـ citrate. بقول لي
+
+53
+00:03:55,820 --> 00:03:59,200
+البكتيريا اللي عندها القدرة على استهلاك الـ sodium
+
+54
+00:03:59,200 --> 00:04:03,420
+citrate، إلى جانب استهلاك هالـ citrate، عندها
+
+55
+00:04:03,420 --> 00:04:09,020
+القدرة على استهلاك الـ ammonia، الـ NH4+. عندها
+
+56
+00:04:09,020 --> 00:04:14,740
+القدرة على إنتاجه، وتحويله لـ ammonia. هذا يعتبر
+
+57
+00:04:14,740 --> 00:04:18,880
+nitrogen source. يبقى صار عندي مصدر للنيتروجين،
+
+58
+00:04:18,880 --> 00:04:23,160
+ومصدر للكربون. هيك البكتيريا بتقدر تنمو وتنقسم،
+
+59
+00:04:23,160 --> 00:04:28,930
+وتتكاثر. هذا بالنسبة لـ in vivo. طيب، in vitro، عشان
+
+60
+00:04:28,930 --> 00:04:33,870
+أكشف هل البكتيريا عندها إنزيم السيترات لياز أو لأ،
+
+61
+00:04:33,870 --> 00:04:38,790
+لازم يكون في الـ media sodium citrate. هي
+
+62
+00:04:38,790 --> 00:04:42,570
+الـ substrates اللي هيشتغل عليها الإنزيم. يبقى in vitro،
+
+63
+00:04:42,570 --> 00:04:46,750
+عشان أكشف عن وجود الإنزيم، بقول لي بتحط في الـ media
+
+64
+00:04:46,750 --> 00:04:50,650
+sodium citrate، الـ substrate، عشان يشتغل عليها الإنزيم.
+
+65
+00:04:50,650 --> 00:04:56,630
+هذه موجودة في الـ media. طيب، الإنزيم citrate lyase،
+
+66
+00:04:56,630 --> 00:05:00,370
+قلنا يعتبر endoenzyme. التفاعل بيصير intra-
+
+67
+00:05:00,370 --> 00:05:03,910
+cellular، داخل الخلية. الـ sodium citrate حجمها مش
+
+68
+00:05:03,910 --> 00:05:08,850
+كبير، الـ substrate حجمها مش كبير، بالتالي الـ enzyme
+
+69
+00:05:08,850 --> 00:05:13,130
+أو الـ substrate، بما أنها موجودة في الـ media، يبقى
+
+70
+00:05:13,130 --> 00:05:18,090
+خارج الخلية. بقولنا لازم تدخل لداخل البكتيريا. احنا
+
+71
+00:05:18,090 --> 00:05:21,850
+قلنا، لو كانت، لو كان الإنزيم endoenzyme، ممكن
+
+72
+00:05:21,850 --> 00:05:24,950
+الـ substrates يكون داخل الخلية، وممكن يكون خارج الخلية.
+
+73
+00:05:24,950 --> 00:05:28,250
+إذا كان داخل الخلية، كان بيحصل التفاعل داخل الخلية.
+
+74
+00:05:28,250 --> 00:05:34,350
+لو كان خارج الخلية، البكتيريا هتفرز إنزيم يدخل
+
+75
+00:05:34,350 --> 00:05:38,870
+الـ substrates لداخل الخلية. بقول لها البكتيريا هتفرز
+
+76
+00:05:38,870 --> 00:05:43,190
+إنزيم الـ citrate permease. هذا هيدخل الـ sodium citrate
+
+77
+00:05:43,190 --> 00:05:47,870
+الموجود في الـ media لداخل الخلية تمامًا. هكذا أصبح الـ
+
+78
+00:05:47,870 --> 00:05:52,450
+substrate موجود داخل الخلية. نكمل الخطوات نفس اللي
+
+79
+00:05:52,450 --> 00:05:57,910
+ذكرناها بالـ in vivo. هيجي citrate lyase يكسر الـ
+
+80
+00:05:57,910 --> 00:06:01,510
+sodium citrate الموجود داخل الخلية. هيسكره
+
+81
+00:06:01,510 --> 00:06:05,990
+لأجزائه، acetic acid و acetate. هيجي الأجزاء acetic
+
+82
+00:06:05,990 --> 00:06:09,930
+acid decarboxylase، هينزع الـ CO2، يتكون بيروفات. هو
+
+83
+00:06:09,930 --> 00:06:14,810
+الـ carbon source. هيظل عندي excess of sodium مع الـ
+
+84
+00:06:14,810 --> 00:06:19,010
+CO2، هيصير في عندي alkaline product، هيرفع لي الـ pH،
+
+85
+00:06:19,010 --> 00:06:22,450
+إلى جانب استهلاكها لـ sodium citrate، هيتم
+
+86
+00:06:22,450 --> 00:06:27,210
+استهلاكها للـ NH4+، هتتحول لأمونيا، تعتبر كـ
+
+87
+00:06:27,210 --> 00:06:31,770
+nitrogen source. يبقى هذا الـ principle، الفحص بيكون
+
+88
+00:06:31,770 --> 00:06:34,970
+عندي بالنهاية الـ pyruvate، اللي هو الـ carbon source،
+
+89
+00:06:34,970 --> 00:06:40,910
+والأمونيا، الـ nitrogen source. Citrate utilization
+
+90
+00:06:40,910 --> 00:06:45,970
+test for the ability of bacteria to convert citrate
+
+91
+00:06:45,970 --> 00:06:50,890
+into oxaloacetate. بقول لي هو هذا الفحص بدي أكشف عن
+
+92
+00:06:50,890 --> 00:06:54,690
+قدرة البكتيريا عن تحويل الـ citrate، تمام، اللي هو
+
+93
+00:06:54,690 --> 00:06:57,870
+يعتبر intermediate في الـ Krebs cycle، لـ
+
+94
+00:06:57,870 --> 00:07:01,450
+oxaloacetate، برضه intermediate آخر في الـ Krebs
+
+95
+00:07:01,450 --> 00:07:05,260
+cycle. هيتحول لـ oxaloacetate، وبعدين التفاعل اللي
+
+96
+00:07:05,260 --> 00:07:10,540
+قلنا بيتحول لبيروفات، لمصدر للـ carbon. هذا الاختبار
+
+97
+00:07:10,540 --> 00:07:14,000
+يستخدم للتفريق بين مجموعات العائلة الـ
+
+98
+00:07:14,000 --> 00:07:18,260
+Enterobacteriaceae. هذه الفقرة هي المهمة لهذا الفحص.
+
+99
+00:07:18,260 --> 00:07:21,960
+هذا الفحص يفرّق بين مين ومين؟ بقول هذا الفحص يفرّق
+
+100
+00:07:21,960 --> 00:07:27,200
+مجموعات الـ Enterobacteriaceae. It is selective for
+
+101
+00:07:27,200 --> 00:07:31,570
+bacteria that have the ability to consume citrate as
+
+102
+00:07:31,570 --> 00:07:34,850
+its sole source of carbon. بقول لي هذه الـ media
+
+103
+00:07:34,850 --> 00:07:38,830
+الـ citrate هنتعرّف عليها كمان شوية. هي selective
+
+104
+00:07:38,830 --> 00:07:44,450
+media. اتعرفنا قبل في المعمل السابق على أنواع الـ
+
+105
+00:07:44,450 --> 00:07:47,970
+media، حسب الـ function. قسمناها إلى general،
+
+106
+00:07:47,970 --> 00:07:51,670
+selective، differential، transport. الـ selective قلنا
+
+107
+00:07:51,670 --> 00:07:56,650
+بتنمي نوع معين من البكتيريا. الـ media الـ citrate
+
+108
+00:07:56,650 --> 00:08:00,460
+فيها citrate فقط، كمصدر
+
+109
+00:08:28,880 --> 00:08:34,600
+استهلاك السيترات، هتكسر السيترات، هتحصل على بيروفات،
+
+110
+00:08:34,600 --> 00:08:37,420
+هيكون هو الـ carbon source اللازم لنمو البكتيريا.
+
+111
+00:08:37,420 --> 00:08:41,360
+يبقى اللي فقط هتنمو على هذه الـ media هي اللي عندها
+
+112
+00:08:41,360 --> 00:08:44,660
+القدرة على استهلاك السيترات. اللي ما عندها القدرة
+
+113
+00:08:44,660 --> 00:08:47,780
+على استهلاك السيترات، اللي ما عندها إنزيمات السيترات
+
+114
+00:08:47,780 --> 00:08:51,560
+lyase، ما هتنمو في هذه الـ media. عشان هيك قال لي
+
+115
+00:08:51,560 --> 00:08:55,720
+selective. Some intestinal bacteria are positive
+
+116
+00:08:55,720 --> 00:09:00,570
+citrate. بقول لي بعض البكتيريا intestinal بتكون
+
+117
+00:09:00,570 --> 00:09:03,730
+positive لهذا الفحص، مثلًا: الكلبسيلا، الـ Enterobacter،
+
+118
+00:09:03,730 --> 00:09:08,110
+الـ Serratia. لكن الـ negative اللي هي الـ E. coli.
+
+119
+00:09:08,110 --> 00:09:14,190
+يبقى هذا هو الـ significant لهذا الفحص. خلينا نشوف
+
+120
+00:09:14,190 --> 00:09:19,520
+هذا الـ significant لهذا الفحص. بقولنا لو عملنا
+
+121
+00:09:19,520 --> 00:09:22,480
+جرام ستين، وطلعت جرام نيجاتيف، بس الـ life يعني دي
+
+122
+00:09:22,480 --> 00:09:26,620
+أكثر من احتمال، ممكن يكون Pseudomonas، Enterobacteriaceae،
+
+123
+00:09:26,620 --> 00:09:33,440
+Vibrio cholerae، Acinetobacter. هنتعرف في فحص
+
+124
+00:09:33,440 --> 00:09:37,340
+الـ oxidase كيف نفرّق منهم. هلأ لو طلعت البكتيريا
+
+125
+00:09:37,340 --> 00:09:41,060
+Enterobacteriaceae، بقول لي Enterobacteriaceae بتنقسم إلى
+
+126
+00:09:41,060 --> 00:09:45,850
+مجموعتين: الكوليفورم، أو بسميها الـ lactose fermenter،
+
+127
+00:09:45,850 --> 00:09:51,050
+الكوليفورم هي بكتيريا موجودة في الأمعاء. الـ non-
+
+128
+00:09:51,050 --> 00:09:55,170
+coliform، أو الـ non-lactose fermenter. بس المجموعة
+
+129
+00:09:55,170 --> 00:09:58,110
+الثانية، الـ non-coliform، أو بسميها الـ non-lactose
+
+130
+00:09:58,110 --> 00:10:02,210
+fermenter. نيجي لمجموعة الكوليفورم، الـ lactose fermenter،
+
+131
+00:10:02,210 --> 00:10:07,150
+بقول بيتقسم برضه لاسمين: fecal، اللي هي مصدرها الـ
+
+132
+00:10:07,150 --> 00:10:09,410
+stool، الـ fecal coliform.
+
+133
+00:10:11,230 --> 00:10:14,590
+coliform، اللي هي الـ E. coli و الـ Klebsiella. لكن
+
+134
+00:10:14,590 --> 00:10:17,530
+الـ non-fecal اللي هي مصدرها مش الـ stool، زي ورق
+
+135
+00:10:17,530 --> 00:10:21,570
+الشجر. الـ Enterobacter و الـ Serratia. لو عملت فحص
+
+136
+00:10:21,570 --> 00:10:26,530
+السيترات، وفحص السيترات، في الكوليفورم، الـ E. coli،
+
+137
+00:10:26,530 --> 00:10:30,450
+negative. لكن باقي الـ Klebsiella، الـ Enterobacter، و
+
+138
+00:10:30,450 --> 00:10:35,270
+الـ Serratia يعتبروا positive في فحص السيترات. ذكرنا
+
+139
+00:10:35,270 --> 00:10:41,090
+سابقا فحص الـ motility. كلهم motile، ما عدا الكلبسيلا،
+
+140
+00:10:41,090 --> 00:10:44,870
+non-motile. يبقى عندي فحصين للتفريق بين الكوليفورم،
+
+141
+00:10:44,870 --> 00:10:47,970
+من الـ lactose fermenter: السيترات، والـ motility.
+
+142
+00:10:47,970 --> 00:10:51,570
+عشان
+
+143
+00:10:51,570 --> 00:10:56,670
+أكشف عن هل البكتيريا عندها إنزيم السيترات لياز أو
+
+144
+00:10:56,670 --> 00:11:00,330
+لأ، عندها القدرة على استهلاك السيترات ولا لأ. بقول لي
+
+145
+00:11:00,330 --> 00:11:04,810
+في عندي طريقتين: الطريقة الأولى اسمها Simons method،
+
+146
+00:11:04,810 --> 00:11:09,930
+الطريقة الثانية اسمها Koser's method. الـ Simons method،
+
+147
+00:11:09,930 --> 00:11:14,550
+بقول لي بستخدم ميديا اسمها Simons citrate agar. هي
+
+148
+00:11:14,550 --> 00:11:20,030
+الميديا، أنا بحضّرها. بعد ما أنا أحضّرها، بـ ... بعمل ...
+
+149
+00:11:20,030 --> 00:11:24,690
+بـ ... بدوبها على الـ hot plate. بعد ما أدوبها، بصوبها في
+
+150
+00:11:24,690 --> 00:11:27,990
+تيوب، بدخلها الـ autoclave تتعقم. بعد ما تطلع من الـ
+
+151
+00:11:27,990 --> 00:11:32,090
+autoclave، الـ autoclave، بدي أخليها تتصلب بزاوية،
+
+152
+00:11:32,090 --> 00:11:36,670
+بدي إياها تكون slant، تمام؟ بعد ما تتصلب في زاوية، بتكون
+
+153
+00:11:36,670 --> 00:11:41,470
+جاهزة للاستخدام. بقول لي عشان تكشفي عن قدرة
+
+154
+00:11:41,470 --> 00:11:45,010
+البكتيريا عن استهلاك السيترات أو لأ، بتجيبي
+
+155
+00:11:45,010 --> 00:11:49,370
+Simons citrate agar، وبتاخدي loop full of البكتيريا،
+
+156
+00:11:49,370 --> 00:11:53,910
+بتعملي كيف؟ أنا بزرع الـ slant، بعمل stabbing، بعدين
+
+157
+00:11:53,910 --> 00:11:58,090
+بزرع دي على الجرار. بعد ما أزرع البكتيريا اللي بدي أكشف
+
+158
+00:11:58,090 --> 00:12:01,290
+قدرتها على استهلاك السيترات أو لأ، بعمل
+
+159
+00:12:01,290 --> 00:12:08,240
+incubation لمدة 24 ساعة، 37 درجة سيلزيوس. صح؟ بقول لي
+
+160
+00:12:08,240 --> 00:12:13,380
+لو نمت البكتيريا، واتحول اللون للون الأزرق، يبقى هي
+
+161
+00:12:13,380 --> 00:12:17,400
+positive للفحص. لكن لو ما نمت البكتيريا، واتحول
+
+162
+00:12:17,400 --> 00:12:21,060
+اللون للون، أو ضلت الـ media لونها أخضر، لون الـ
+
+163
+00:12:21,060 --> 00:12:24,360
+media لونها أخضر، لو ضلّ الـ media لونها أخضر، يبقى هي
+
+164
+00:12:24,360 --> 00:12:29,080
+negative لهذا الفحص. عشان نفهم أكثر، ليش اتحول الـ
+
+165
+00:12:29,080 --> 00:12:32,180
+لون، وليش في عندي growth في النتيجة الـ positive،
+
+166
+00:12:32,180 --> 00:12:36,220
+بنعرف على مكونات الـ media. بما إنها agar،
+
+167
+00:12:36,220 --> 00:12:40,820
+بقولنا نسبة الـ agar فيها بيكون 1.5%. في عندي sodium
+
+168
+00:12:40,820 --> 00:12:45,180
+citrate و NH4+ كـ substrates اللي هيشتغل عليهم
+
+169
+00:12:45,180 --> 00:12:49,320
+الـ enzyme، الـ citrate lyase، عشان أحصل على carbon و
+
+170
+00:12:49,320 --> 00:12:53,060
+nitrogen source. ما في عندي أي مصدر آخر للكربون
+
+171
+00:12:53,060 --> 00:12:55,740
+والنيتروجين، ما في عندي beef extract ولا peptone،
+
+172
+00:12:55,74
+
+223
+00:16:45,340 --> 00:16:49,520
+موجودة بوتيل بحضرها الـ broth سائلة بجيب loop full
+
+224
+00:16:49,520 --> 00:16:53,420
+of الـ bacteria البكتيريا اللي بتكشف عندها انزيم
+
+225
+00:16:53,420 --> 00:16:56,080
+السيطرات لياز أو لأ انها القدرة على استهلاك
+
+226
+00:16:56,080 --> 00:16:59,780
+السيطرات أو لأ بعمل mix بعمل incubation لمدة 24
+
+227
+00:16:59,780 --> 00:17:04,960
+ساعة على 37 درجة سيليزية. إذا كان في عندي نمو يبقى
+
+228
+00:17:04,960 --> 00:17:10,480
+هي positive للفحص. إذا مافي عندي نمو يبقى مافي
+
+229
+00:17:10,480 --> 00:17:15,620
+عندي نمو يبقى negative للفحص بالنسبة للنتيجة الـ
+
+230
+00:17:15,620 --> 00:17:19,440
+growth الـ growth في الـ broth قولنا إذا بتتذكروا في
+
+231
+00:17:19,440 --> 00:17:23,180
+معمل الـ media preparation قولنا أنواع النمو في الـ
+
+232
+00:17:23,180 --> 00:17:29,040
+broth ممكن يكون turbidity أو ممكن يكون pellicle أو
+
+233
+00:17:29,040 --> 00:17:34,070
+ممكن يكون sediment. فبالتالي أي شكل من هذه الأشكال
+
+234
+00:17:34,070 --> 00:17:39,290
+يعتبر أنه في عندي Growth. هذا أشكال أنه مافي البروث
+
+235
+00:17:39,290 --> 00:17:42,810
+مكونات الـ media بما أنها البروث مافي عندي أجار
+
+236
+00:17:42,810 --> 00:17:47,630
+في عندي Sodium Citrate و NH4 Plus كـ substrate هذه
+
+237
+00:17:47,630 --> 00:17:51,110
+هتكون مصدر للكربون. مصدر وحيد للكربون. هذه هتكون
+
+238
+00:17:51,110 --> 00:17:56,310
+مصدر وحيد للنيتروجين. NACL الـ Normal Saline هو
+
+239
+00:17:57,380 --> 00:18:00,420
+هيحفظلي على الـ Osmotic Pressure. مافي عندي BH
+
+240
+00:18:00,420 --> 00:18:05,080
+Indicator في هذه الميديا. فبالتالي ما هيكون في عندى
+
+241
+00:18:05,080 --> 00:18:08,900
+اختلاف في اللون. فقط إذا وجود النموم هو اللي
+
+242
+00:18:08,900 --> 00:18:14,330
+هيكشفلي عن هذا الانزيم. يبقى في عندي فروقات بين
+
+243
+00:18:14,330 --> 00:18:18,490
+الطريقتين. أول فرق أن هاي الطريقة فيها أجار لكن هاي
+
+244
+00:18:18,490 --> 00:18:22,630
+الطريقة. هاي الـ media الـ causer سيطرات هي الـ growth
+
+245
+00:18:22,630 --> 00:18:28,070
+الاختلاف التاني أن هان في عندي pH indicator هان
+
+246
+00:18:28,070 --> 00:18:31,970
+مافي عندي pH indicator. هان الـ result هيكون في عندي
+
+247
+00:18:31,970 --> 00:18:37,260
+نمو بعد تغير اللون لكن هان فقط هيكون نمو. طبعاً
+
+248
+00:18:37,260 --> 00:18:42,380
+هتنمو البكتيريا لأنه لو كان عندها أنزيمات أو لو
+
+249
+00:18:42,380 --> 00:18:46,200
+كان عندها القدرة على استهلاك السييتريات هتحول الـ
+
+250
+00:18:46,200 --> 00:18:53,140
+Sodium هتكسر السييتريات لـ Oxaloacetic Acid. هيتحول
+
+251
+00:18:53,140 --> 00:18:56,680
+بواسطة أنزيم الـ Oxaloacetic Acid D-Carboxylase
+
+252
+00:18:56,680 --> 00:19:00,780
+لـ pyruvate مصدر للكربون لازم لنمو البكتيريا
+
+253
+00:19:00,780 --> 00:19:04,560
+بالإضافة لاستهلاكها للسييتريات هتستهلك الـ N-H4
+
+254
+00:19:04,560 --> 00:19:08,910
+Plus لأمونيا. فبالتالي صار في عندي مصدر للـ carbon و
+
+255
+00:19:08,910 --> 00:19:13,470
+الـ nitrogen. هتنمو البكتيريا و تتكاثر و يكون في
+
+256
+00:19:13,470 --> 00:19:17,710
+عندي turbidity او sediment او pellicle. لكن لو كان
+
+257
+00:19:17,710 --> 00:19:20,950
+ما عندها. مش هيكون في عندي اي نمو لأن مافي عندي اي
+
+258
+00:19:20,950 --> 00:19:25,370
+مصادر اخرى للـ carbon او الـ nitrogen. هذا بالنسبة
+
+259
+00:19:25,370 --> 00:19:31,590
+لطريقة الكشف عن السيطرات utilization. نيجي لـ السيمون
+
+260
+00:19:31,590 --> 00:19:35,290
+سايتريات أجار. organism that metabolize citrate
+
+261
+00:19:35,290 --> 00:19:38,430
+utilize the ammonium salt releasing ammonia and
+
+262
+00:19:38,430 --> 00:19:42,030
+increasing the pH of the media. بقوللي البكتيريا
+
+263
+00:19:42,030 --> 00:19:45,050
+اللي عندها القدرة على استهلاك السايتريات. يعني ده
+
+264
+00:19:45,050 --> 00:19:47,850
+القدرة على استهلاك الـ ammonium salt اللي هي الـ N
+
+265
+00:19:47,850 --> 00:19:51,630
+-H4 plus. بالتالي لما تستهلك الـ ammonium salt
+
+266
+00:19:51,630 --> 00:19:56,130
+هتكون الأمانيا. الأمانيا هترفعلي الـ pH في الميديا
+
+267
+00:19:56,130 --> 00:20:00,120
+في برامو ثيمول بلو موجود في الـ media as
+
+268
+00:20:00,120 --> 00:20:05,080
+indicator. زي BH indicator. بقول هيحول اللون لـ
+
+269
+00:20:05,080 --> 00:20:10,080
+green لـ neutral. BH قولنا إنه اللي فيه الـ neutral
+
+270
+00:20:10,080 --> 00:20:15,640
+BH هيكون اللون أزرق. بما إن الـ BH ارتفع بالتالي
+
+271
+00:20:15,640 --> 00:20:20,760
+هيتحول اللون للون أزرق blue. لما يكون أعلى من 7.6
+
+272
+00:20:21,600 --> 00:20:24,960
+سيمون سايتريات أجار بيعرف لـ سيمون سايتريات أجار
+
+273
+00:20:24,960 --> 00:20:27,940
+بتحتوي على صوديوم سايتريات as a sole carbon source
+
+274
+00:20:27,940 --> 00:20:31,440
+كمصدر واحد للكربون. امونيوم ايون كمصدر واحد
+
+275
+00:20:31,440 --> 00:20:35,620
+للنيتروجين. and برامو ثمال بلو. لو ما كتبت برامو
+
+276
+00:20:35,620 --> 00:20:39,940
+ثمال بلو. بتتحول الـ media لـ causer citrate broth
+
+277
+00:20:39,940 --> 00:20:44,120
+يبقى هذه بتحتوي على مصدر كربون واحد. مصدر نيتروجين
+
+278
+00:20:44,120 --> 00:20:49,320
+واحد. وبرامو ثمال بلو. كيف طريقة الشغل قلنا نستخدم
+
+279
+00:20:49,320 --> 00:20:53,080
+inoculating needle عشان نعمل stabbing. بعدين بعمل
+
+280
+00:20:53,080 --> 00:21:01,160
+zigzag بزرع الـ slant. بيعمل incubation لمدة 24 ساعة
+
+281
+00:21:01,160 --> 00:21:05,920
+على 37 درجة السليسيس. بشوف الـ growth و التغير في
+
+282
+00:21:05,920 --> 00:21:10,280
+اللون. النتيجة الـ positive بشوفها بكون في عندي no
+
+283
+00:21:10,280 --> 00:21:14,040
+more. مع تغير في اللون للون الأزرق. والـ growth on
+
+284
+00:21:14,040 --> 00:21:17,820
+the media is combined by a rise in a pH to change
+
+285
+00:21:17,820 --> 00:21:20,800
+the media from its initial green color to deep
+
+286
+00:21:20,800 --> 00:21:25,180
+blue. والـ growth هيكون في الميديا. وكمان هيكون الـ
+
+287
+00:21:25,180 --> 00:21:30,940
+pH هيتغير في الميديا من green لـ blue. نلاحظ هنا في
+
+288
+00:21:30,940 --> 00:21:34,620
+عندي control. negative control. لون الميديا ضاله لون
+
+289
+00:21:34,620 --> 00:21:41,000
+green أخضر. الأزرق هنا عندي الـ Enterobacter الـ
+
+290
+00:21:41,000 --> 00:21:44,100
+Enterobacter قولنا positive للسيطرات. يبقى هيتحول
+
+291
+00:21:44,100 --> 00:21:47,800
+اللون للون أزرق. Equally قولنا negative يبقى هيدل
+
+292
+00:21:47,800 --> 00:21:51,100
+اللون green. Eclipsella قولنا positive للسيطرات
+
+293
+00:21:51,100 --> 00:21:55,510
+يبقى هيتحول اللون للون blue. الطريقة التانية الـ
+
+294
+00:21:55,510 --> 00:21:59,710
+Causar citrate broth is another medium used to
+
+295
+00:21:59,710 --> 00:22:03,270
+test for citrate utilization. طريقة أخرى للكشف على
+
+296
+00:22:03,270 --> 00:22:06,810
+الـ citrate utilization. Causar citrate broth قلنا
+
+297
+00:22:06,810 --> 00:22:10,690
+is based on this formulation and contains Sodium
+
+298
+00:22:10,690 --> 00:22:13,630
+citrate as a single source of carbon. بتحتوي على
+
+299
+00:22:13,630 --> 00:22:17,690
+Sodium citrate كمصدر وحيد للكربون. Ammonium salt
+
+300
+00:22:17,930 --> 00:22:21,470
+كمصدر وحيد للنيتروجين. مش موجود فيه عندي BH
+
+301
+00:22:21,470 --> 00:22:24,930
+Indicator. يبقى هذه الـ Causar Citrate البروث
+
+302
+00:22:24,930 --> 00:22:28,490
+بكتيريا قادرة على استخدام الـ Citrate كمصدر كربون
+
+303
+00:22:28,490 --> 00:22:32,110
+هارس ستنمو في الميديا ويسبب تربيدتي. بقول البكتيريا
+
+304
+00:22:32,110 --> 00:22:35,190
+اللي عندها القدرة على استهلاك الـ Citrate كمصدر
+
+305
+00:22:35,190 --> 00:22:38,410
+وحيد للكربون هتنمو في الميديا وهذا هيسبب تربيدتي
+
+306
+00:22:38,410 --> 00:22:41,670
+قلنا مش بس تربيدتي ممكن يكون sediment وممكن يكون
+
+307
+00:22:41,670 --> 00:22:46,550
+pellicle. لأنه أشكال النمو في البروث تيوب. تربيدتي
+
+308
+00:22:46,550 --> 00:22:51,030
+بيليكل سيدمان. النتيجة الـ Positive Growth كافة ان
+
+309
+00:22:51,030 --> 00:22:54,930
+مو تربدتي زائد sediment زائد pellicle. الـ Negative
+
+310
+00:22:54,930 --> 00:22:59,090
+No growth هتكون clear. مافي عندي اي تغير في اللون
+
+311
+00:22:59,090 --> 00:23:04,410
+خلصنا من الـ citrate utilization. يُعتبر endoenzyme
+
+312
+00:23:04,410 --> 00:23:09,210
+نيجي للـ Oxidase test. الـ Oxidase test يُعتبر أو الـ
+
+313
+00:23:09,210 --> 00:23:13,780
+Oxidase enzyme أيضا يُعتبر endoenzyme. بقول لي the
+
+314
+00:23:13,780 --> 00:23:16,940
+oxidase test is used to identify bacteria
+
+315
+00:23:16,940 --> 00:23:20,220
+containing the respiratory enzyme cytochrome-c
+
+316
+00:23:20,220 --> 00:23:24,280
+oxidase. يبقى بدي أكشف هل عند البكتيريا إنزيم الـ
+
+317
+00:23:24,280 --> 00:23:27,940
+cytochrome-c oxidase ولا لأ. اسمه هك لأن الـ
+
+318
+00:23:27,940 --> 00:23:30,220
+substrate اللي هيشتغل عليها الـ enzyme هي الـ
+
+319
+00:23:30,220 --> 00:23:34,480
+cytochrome-c. يبقى الـ substrate الـ cytochrome-c
+
+320
+00:23:34,480 --> 00:23:39,000
+الإنزيم اللي بدي أكشف عليه. أكسداز. كيف بتكون الـ
+
+321
+00:23:39,000 --> 00:23:42,460
+cytochrome-C؟ بقول الـ cytochrome-C oxidase is the
+
+322
+00:23:42,460 --> 00:23:45,540
+terminal enzyme in the electron transport chain
+
+323
+00:23:45,540 --> 00:23:48,780
+بقول لي هو الانزيم النهائي في الـ electron
+
+324
+00:23:48,780 --> 00:23:52,960
+transport chain. the enzyme which reduce molecular
+
+325
+00:23:52,960 --> 00:23:56,080
+oxygen to water to complete the last link in the
+
+326
+00:23:56,080 --> 00:24:01,480
+chain of aerobic respiration. بتكون في نهاية اللي هو
+
+327
+00:24:01,480 --> 00:24:06,600
+عملية التنفس الخلوي. في نهاية الـ Krebs Cycle بيحدث
+
+328
+00:24:06,600 --> 00:24:09,900
+Redox Reaction اللي هو Oxidation Reduction
+
+329
+00:24:09,900 --> 00:24:14,780
+Reaction. الـ Cytochrome C oxidase موجود في الـ
+
+330
+00:24:14,780 --> 00:24:17,500
+Electron Transport Exchange. هذا الإنزين بيقول
+
+331
+00:24:17,500 --> 00:24:23,190
+بيعمل Reduce لـ Molecular Oxygen. لوطة عشان نفهم
+
+332
+00:24:23,190 --> 00:24:26,590
+أكتر. قولنا الـ cytochrome-C oxidase بيشتغل على الـ
+
+333
+00:24:26,590 --> 00:24:29,510
+substrates اللي هي الـ cytochrome-C أو بسميها
+
+334
+00:24:29,510 --> 00:24:34,670
+reduced cytochrome-C.  اش يعني reduced يعني مختزل
+
+335
+00:24:34,670 --> 00:24:38,270
+يعني مقتسب إلكترونات. الـ cytochrome-C oxidase من
+
+336
+00:24:38,270 --> 00:24:43,470
+اسمه هيعمل oxidation لـ الـ reduced cytochrome-C
+
+337
+00:24:43,470 --> 00:24:47,730
+كانت مكتسبة إلكترونات هتصير فاقدة للإلكترونات
+
+338
+00:24:47,730 --> 00:24:52,920
+هتتحول لأكسداز cytochrome-C. بما أنها فقدت إلكترون
+
+339
+00:24:52,920 --> 00:24:57,720
+يبقى الإلكترون هيروح لإيش تاني؟ لمركب تاني
+
+340
+00:24:57,720 --> 00:25:02,640
+الإلكترون اللي فقدته. بقولي هتاخده الـ O2 الـ
+
+341
+00:25:02,640 --> 00:25:06,940
+Molecular Oxygen. الـ O2 مع الـ H Plus. قولنا في
+
+342
+00:25:06,940 --> 00:25:10,680
+عندي مضخات لترانسبورت الإلكترون. الترانسبورت تتشهد
+
+343
+00:25:10,680 --> 00:25:15,880
+مضخات للـ H Plus. يبقى عندي H Plus مع الـ Molecular
+
+344
+00:25:15,880 --> 00:25:21,590
+Oxygen. في عندي إلكترون تم فقده خلال عملية الـ
+
+345
+00:25:21,590 --> 00:25:25,410
+Oxidation لـ Cytochrome-C. لـ Reduce Cytochrome-C
+
+346
+00:25:25,410 --> 00:25:31,570
+إلكترون زائد H بلس زائد O2 هيتكون عندي H2O. يبقى
+
+347
+00:25:31,570 --> 00:25:37,930
+هذا هو الـ issue اللي بيصير in vivo. in vivo بقولي
+
+348
+00:25:37,930 --> 00:25:42,690
+إن Cytochrome-C Oxidase هيعمل Oxidation لـ Reduce
+
+349
+00:25:42,690 --> 00:25:47,440
+Cytochrome-C. Reduce يعني مختزل يعني مكتز بإلكترونات
+
+350
+00:25:47,440 --> 00:25:50,640
+بما أنه صار له أكسدة يبقى فقد الإلكترونات. صار
+
+351
+00:25:50,640 --> 00:25:54,960
+Oxidized Cytochrome-C. الإلكترون وين هيروح؟
+
+352
+00:25:54,960 --> 00:25:59,100
+الإلكترون بقولّي هيرتبط مع الـ Molecular Oxygen مع
+
+353
+00:25:59,100 --> 00:26:02,080
+الـ H Plus اللي هي موجود في عندي مدخّات في
+
+354
+00:26:02,080 --> 00:26:04,880
+الإلكترون Transporter Chain. هيرتبطوا مع بعض
+
+355
+00:26:04,880 --> 00:26:10,080
+ويكونوني الـ H2O. يبقى هيك صار في عنده التفاعل in
+
+356
+00:26:10,080 --> 00:26:11,680
+vivo. طيب
+
+357
+00:26:19,790 --> 00:26:23,870
+عشان أنا أكشف هل عندي انزيم الـ Oxidase ولا لأ
+
+358
+00:26:23,870 --> 00:26:28,570
+بقوللي بجيب Substrate بتشبه الـ Cytochrome-C. والله
+
+359
+00:26:28,570 --> 00:26:32,630
+لو عندي انزيم الـ Oxidase موجود في البكتيريا هتعمل
+
+360
+00:26:32,630 --> 00:26:37,150
+Oxidation للـ Cytochrome-C. هتتحول لـ Oxidase
+
+361
+00:26:37,150 --> 00:26:41,170
+Cytochrome-C. هتتحول للون تاني بقدر اني انا اكشف
+
+362
+00:26:41,170 --> 00:26:45,910
+عنه. هنتعرف هلأ في الـ Principle. بقوللي Oxidase
+
+363
+00:26:45,910 --> 00:26:50,970
+producing bacteria. الأمثلة على البكتيريا Oxidase
+
+364
+00:26:50,970 --> 00:26:54,770
+Positive. Pseudomonas. Neisseria. Vibrio. Aeromonas.
+
+365
+00:26:54,770 --> 00:26:59,270
+Pseudomonas aeruginosa is a gram negative بكتيريا
+
+366
+00:26:59,270 --> 00:27:02,270
+Pseudomonas aeruginosa هي gram negative بس
+
+367
+00:27:02,270 --> 00:27:07,450
+aerobic rod. هي aerobic هوائية. have a strictly
+
+368
+00:27:07,450 --> 00:27:11,410
+respiratory type of metabolism with oxygen as the
+
+369
+00:27:11,410 --> 00:27:14,370
+terminal electron acceptor. and this is أكسداز
+
+370
+00:27:14,370 --> 00:27:17,990
+positive. بقوللي إن صلمانوس إيروجينازا هي أكسداز
+
+371
+00:27:17,990 --> 00:27:22,210
+positive. طالما أكسداز positive طبيعي إنها تكون
+
+372
+00:27:22,210 --> 00:27:25,870
+obligate aerobic. هوائية إجبارية. هوائية إجبارية
+
+373
+00:27:25,870 --> 00:27:30,930
+يعني تستخدم الأكسجين كمستقبل نهائي للإلكترونات
+
+374
+00:27:30,930 --> 00:27:39,100
+بالتالي هيصله هيكتسب إلكترون يتحول لـ H2O. هيصير أكسدة
+
+375
+00:27:39,100 --> 00:27:43,060
+للـ «cytochrome-c» ويتحول لأكسداء الـ «cytochrome
+
+376
+00:27:43,060 --> 00:27:48,560
+-c» لكن الأكسجن هيصله اختزال وهيكتسب إلكترون
+
+377
+00:27:48,560 --> 00:27:53,360
+وهيتحول لـ H2O. الانتيرو بكترياسي بقول لي is Oxidase
+
+378
+00:27:53,360 --> 00:27:57,180
+negative bacteria. يبقى هي Oxidase negative. لكن الـ
+
+379
+00:27:57,180 --> 00:28:01,100
+Pseudomonas هي Oxidase positive. عشان أفرق الانتيرو
+
+380
+00:28:01,100 --> 00:28:04,040
+بكترياسي عن الـ Pseudomonas بعمل فحص الـ Oxidase. لو
+
+381
+00:28:04,040 --> 00:28:07,380
+طلعت positive. التنتين طبعا هم إجرام نيجاتيف بصلية
+
+382
+00:28:07,380 --> 00:28:10,760
+Pseudomonas و الانتيرو بكترياسي إجرام نيجاتيف بصلية
+
+383
+00:28:11,040 --> 00:28:14,140
+عشان أفرق بينهم بعمل فحص الـ Oxidase. إذا طلعت
+
+384
+00:28:14,140 --> 00:28:17,100
+Negative يبقى هي Enterobacteriasy. إذا طلعت
+
+385
+00:28:17,100 --> 00:28:21,120
+Positive يبقى هي Pseudomonas. في فحص تاني اللي هو الـ
+
+386
+00:28:21,120 --> 00:28:24,860
+Gelicose Fermenter. هنتعرف عليه في الـ chapter الجاي.
+
+387
+00:28:24,860 --> 00:28:29,720
+Pseudomonas aeruginosa هي Non-Gelicose Fermenter. لكن
+
+388
+00:28:29,720 --> 00:28:33,300
+الـ Enterobacteriasy هي Gelicose Fermenter. يبقى
+
+389
+00:28:33,300 --> 00:28:36,880
+هدول الفحصين بيفرقوا لبين الـ Enterobacteriasy و
+
+390
+00:28:36,880 --> 00:28:37,820
+Pseudomonas.
+
+391
+00:28:40,250 --> 00:28:43,910
+كيف أنا بدي أكشف عن هذا الانزيم in vitro؟ قلنا
+
+392
+00:28:43,910 --> 00:28:47,410
+بنجيب substrate تشبه الـ substrate اللي بيشتغل
+
+393
+00:28:47,410 --> 00:28:51,530
+عليها الـ enzyme. الـ Oxidase test ممكن يكون فيه اندي
+
+394
+00:28:51,530 --> 00:28:55,630
+reagent. ممكن يكون strip و ممكن يكون disk. بقولي الـ
+
+395
+00:28:55,630 --> 00:28:58,130
+reagent او الـ substrate اللي هيشتغل عليها
+
+445
+00:32:39,840 --> 00:32:43,620
+لأن ممكن بعدها الـ reagent يتأثر بالأكسجين الموجود
+
+446
+00:32:43,620 --> 00:32:46,840
+بالهواء ويعطيني false positive result واللي في
+
+447
+00:32:46,840 --> 00:32:50,740
+عندي طريقة بديلة أني أنا بجيب الـ oxidase reagent
+
+448
+00:32:50,740 --> 00:32:56,220
+الـ reagent اللي هيمحضر بيكون و بديروه بشكل مباشر
+
+449
+00:32:56,220 --> 00:32:59,600
+على الكولوني الموجودة في الأجار لو اتحول اللون للون
+
+450
+00:32:59,600 --> 00:33:03,640
+بيربول يبقى النتيجة بتكون positive، ملاحظاتها ما
+
+451
+00:33:03,640 --> 00:33:06,880
+بقول لي pick your inoculum not with a metal loop، و
+
+452
+00:33:06,880 --> 00:33:09,960
+ممنوع تستخدم الـ metal loop، لازم تستخدم الـ plastic
+
+453
+00:33:09,960 --> 00:33:16,020
+loop أو الـ wooden stick ل pick، ليش؟ لأن الـ
+
+454
+00:33:16,020 --> 00:33:18,840
+reaction ممكن يتفاعل مع الـ metal ويعطيني false
+
+455
+00:33:18,840 --> 00:33:26,020
+positive result،  ونبقي الحرارة مُفرومة أو غير مفتوحة
+
+456
+00:33:26,020 --> 00:33:29,830
+في الحجم حتى تكون محتاجة، old reagent is sitting out
+
+457
+00:33:29,830 --> 00:33:33,010
+on the bench and is unbearable, ask for a new tube
+
+458
+00:33:33,010 --> 00:33:37,350
+from the instructor، بقول لي الـ reagent اللي
+
+459
+00:33:37,350 --> 00:33:43,370
+بتستخدميه بتخليه بالتلاجة، بتخليه مسكر، ممنوع تخليه
+
+460
+00:33:43,370 --> 00:33:49,130
+مفتوح إلا عند الحاجة، بتستخدميه لو كان old reagent
+
+461
+00:33:49,130 --> 00:33:53,810
+ممنوع أنك تستخدميه، لأنه ممكن يتغير مع العوامل
+
+462
+00:33:53,810 --> 00:33:58,170
+الخارجية ويعطيني false result، ضروري أنا أي فحص
+
+463
+00:33:58,170 --> 00:34:01,070
+كيميائي أستخدم young culture أو fresh culture
+
+464
+00:34:01,070 --> 00:34:04,110
+ممنوع أنا أستخدم old culture، لأن الـ enzyme activity
+
+465
+00:34:04,110 --> 00:34:08,130
+بيقل مع الوقت، قلنا fresh reagent لازم أنا أستخدم الـ
+
+466
+00:34:08,130 --> 00:34:12,130
+reagent يكون fresh، آخده من الـ freezer، ممنوع يكون
+
+467
+00:34:12,130 --> 00:34:16,090
+open، مفتوح في الـ tube، لأن قلنا الـ reagent ممكن
+
+468
+00:34:16,090 --> 00:34:19,850
+يتغير مع العوامل الخارجية ويعطيني false positive
+
+469
+00:34:19,850 --> 00:34:24,490
+result، بقرأ النتيجة خلال عشرين second، not after
+
+470
+00:34:24,490 --> 00:34:29,130
+ليش؟ لأن ممكن أن الأكسجين يتفاعل مع الـ reagent
+
+471
+00:34:29,130 --> 00:34:32,130
+ويعطيني false positive result، عشان هيك بقرأ
+
+472
+00:34:32,130 --> 00:34:38,330
+النتيجة خلال 20 ثانية، موريكسيلا كاتاراليس and
+
+473
+00:34:38,330 --> 00:34:41,010
+نيسيريا are gram-negative diplococci
+
+474
+00:34:41,010 --> 00:34:43,430
+يتعرفنا على الـ gram-negative بس اللي يقول لنا اللي
+
+475
+00:34:43,430 --> 00:34:45,950
+هي الـ Pseudomonas والـ Enterobacteriaceae، كذا بفرق
+
+476
+00:34:45,950 --> 00:34:49,850
+بينهم، وبفحص الأكسيداز وفحص الجلوكوز fermentation
+
+477
+00:34:50,520 --> 00:34:53,540
+بندى لجرام نيجاتيف ديبلوكوكاي اللي هي الموريكس
+
+478
+00:34:53,540 --> 00:34:57,380
+الكاتاراليس ونيسيريا، بنلقى إنهم أكسيداز positive و
+
+479
+00:34:57,380 --> 00:35:01,140
+كاتاليز positive، كيف أفرق بينهم؟ بندي فحص الـ
+
+480
+00:35:01,140 --> 00:35:05,060
+بيوتيرات استراز، بيوتيرات استراز بيكشف ليه؟ عن إنزيم الـ
+
+481
+00:35:05,060 --> 00:35:10,380
+بيوتيرات استيريز، إنزيم هذا، الإنزيم موجود في
+
+482
+00:35:10,380 --> 00:35:14,580
+الموريكزيلا كاتاراليس و positive في الموريكزيلا
+
+483
+00:35:14,580 --> 00:35:17,560
+كاتاراليس و negative في النيسيريا، يبقى اللي بدنا
+
+484
+00:35:17,560 --> 00:35:20,900
+نعرفه إن أنا عشان أفرق الموريكزيلا عن النيسيريا في
+
+485
+00:35:20,900 --> 00:35:24,360
+عندي إنزيم أو فحص الـ biothiorate test بيكشف على
+
+486
+00:35:24,360 --> 00:35:27,700
+إنزيم الـ biothiorate استراز enzyme، positive في
+
+487
+00:35:27,700 --> 00:35:32,210
+الموريكزيلا كاتاراليس و negative في النيسيريا، هذا الـ
+
+488
+00:35:32,210 --> 00:35:35,530
+slide، كملنا، قلنا لو كان فيه عندي جرام ستين ممكن
+
+489
+00:35:35,530 --> 00:35:38,070
+تكون جرام positive أو negative، الجرام positive و
+
+490
+00:35:38,070 --> 00:35:42,270
+negative، كل منها بنقسمها لكوكاي وبصلاي، لو كانت
+
+491
+00:35:42,270 --> 00:35:45,790
+جرام positive بصلاي بعمل فحص الكاتاليز، إذا طلعت
+
+492
+00:35:45,790 --> 00:35:47,850
+negative يبقى ستربتو، إذا طلعت positive يبقى
+
+493
+00:35:47,850 --> 00:35:51,770
+استافيلوكوكاي، ماكروكوكاي، مايكروكوكاي، كيف بدي
+
+494
+00:35:51,770 --> 00:35:56,230
+أفرق بينهم؟ بفحص الأكسيداز، فحص الأكسيداز لو طلعت
+
+495
+00:35:56,230 --> 00:35:59,990
+أكسيداز positive يبقى هي ماكرو أو مايكرو، لو طلعت
+
+496
+00:35:59,990 --> 00:36:03,390
+أكسيداز negative يبقى هي ستافيلوكوكاي، طب كيف بدي
+
+497
+00:36:03,390 --> 00:36:06,670
+أفرق الـ Staphylococci species عن بعض؟ بقول بفحص الـ
+
+498
+00:36:06,670 --> 00:36:09,690
+coagulase، لو coagulase positive يبقى هي
+
+499
+00:36:09,690 --> 00:36:12,910
+Staphylococcus aureus، لو coagulase negative يبقى
+
+500
+00:36:12,910 --> 00:36:15,670
+هي Staphylococcus epidermidis أو Staphylococcus
+
+501
+00:36:15,670 --> 00:36:20,070
+saprophyticus، بالنسبة لـ Gram-positive Bacilli بعمل
+
+502
+00:36:20,070 --> 00:36:22,710
+فحص الكاتاليز، لو طلعت positive يبقى بيبقى باصيلس
+
+503
+00:36:22,710 --> 00:36:26,570
+أو الـ Listeria monocytogenes، لو طلعت negative يبقى هي
+
+504
+00:36:26,570 --> 00:36:29,750
+Clostridium، وكيف بدي أفرق الباسيلس عن الـ Listeria monocytogenes؟
+
+505
+00:36:29,750 --> 00:36:32,630
+بالأبواغ، الباسيلس spore forming،
+
+506
+00:36:32,630 --> 00:36:37,550
+الليستريا مونوسايتوجينز non-spore forming، الجرام
+
+507
+00:36:37,550 --> 00:36:41,550
+نيجاتيف ديبلوكوكاي، كاتاليز والأكسيداز لهم positive
+
+508
+00:36:41,550 --> 00:36:44,230
+اللي هي الموريكزيلا والنيسيريا، عشان أفرق بينهم
+
+509
+00:36:44,230 --> 00:36:47,490
+بعمل فحص الـ butyrate esterase، الـ positive
+
+510
+00:36:47,490 --> 00:36:50,770
+الموريكزيلا والـ negative النيسيريا، الجرام نيجاتيف
+
+511
+00:36:50,770 --> 00:36:56,810
+باسلاي، الجرام نيجاتيف باسلاي بتضم الـ Pseudomonas
+
+512
+00:36:56,810 --> 00:37:00,050
+الهيليكوباكترياسيا وكمان في عندي الـ Acinetobacter، الـ
+
+513
+00:37:00,050 --> 00:37:03,970
+Vibrio، الهيليكوباكتر بقول عشان أفرق بينهم بعمل فحص
+
+514
+00:37:03,970 --> 00:37:06,930
+الأكسيداز، لو طلعت positive يبقى احتمال عندي لو
+
+515
+00:37:06,930 --> 00:37:11,270
+طلعت أكسيداز positive إما Pseudomonas أو Vibrio، لو
+
+516
+00:37:11,270 --> 00:37:14,510
+طلعت أكسيداز negative قلنا الهيليكوباكترياسيا في
+
+517
+00:37:14,510 --> 00:37:18,070
+كمان الـ Acinetobacter، كيف بدي أفرق الـ Pseudomonas عن الـ
+
+518
+00:37:18,070 --> 00:37:22,640
+Vibrio؟ بقول بفحص الجلوكوز، لو طلعت negative يبقى هي
+
+519
+00:37:22,640 --> 00:37:25,560
+Pseudomonas، لو طلعت positive هي Vibrio أو
+
+520
+00:37:25,560 --> 00:37:29,580
+Helicobacter، كيف أفرق الـ Acinetobacter و الـ
+
+521
+00:37:29,580 --> 00:37:32,100
+Enterobacteriaceae؟ لو طلع الـ Oxidase negative بقول لي
+
+522
+00:37:32,100 --> 00:37:35,020
+برضه بفحص الجلوكوز fermenter، لو طلعت negative فهي
+
+523
+00:37:35,020 --> 00:37:38,000
+Acinetobacter، لو طلعت positive يبقى هي
+
+524
+00:37:38,000 --> 00:37:40,780
+Enterobacteriaceae، الـ Enterobacteriaceae قلنا عائلة
+
+525
+00:37:40,780 --> 00:37:43,620
+كبيرة ممكن إنها تكون بتنقسم لـ Lactose fermenter و
+
+526
+00:37:43,620 --> 00:37:47,310
+Non-lactose fermenter، لو طلعت Lactose Fermenter
+
+527
+00:37:47,310 --> 00:37:51,770
+بتنقسم لـ fecal و non-fecal، الـ fecal فيه عندي الـ E.coli و E.
+
+528
+00:37:51,770 --> 00:37:53,630
+klebsiella، الـ non-fecal للـ Enterobacter
+
+529
+00:37:53,630 --> 00:37:57,510
+والـ Serratia، كيف بفرق بينهم؟ بفحص السيترات، E.coli
+
+530
+00:37:57,510 --> 00:38:00,250
+negative لكن الباقي positive، أو فحص الـ motility،
+
+531
+00:38:00,250 --> 00:38:03,450
+الـ E.klebsiella non-motile، والباقي motile، يبقى هذا
+
+532
+00:38:03,450 --> 00:38:08,790
+هو هيك تقريباً، حاولنا أن نلخص الفحوصات عشان نتعرف
+
+533
+00:38:08,790 --> 00:38:14,590
+على البكتيريا، هذه هي الـ slide، الـ oxidase slide test،
+
+534
+00:38:14,590 --> 00:38:17,930
+هاي الـ slide بتكون مغمورة بالـ reagent، بالـ
+
+535
+00:38:17,930 --> 00:38:22,290
+substrate، لما أنا آخد البكتيريا، أخدت البكتيريا وحطيتها
+
+536
+00:38:22,290 --> 00:38:25,670
+على هاي الـ slide، صار اللون أو ما تغير، كان
+
+537
+00:38:25,670 --> 00:38:29,570
+colorless يبقى هي oxidase negative بكتيريا، لكن لو
+
+538
+00:38:29,570 --> 00:38:32,970
+اتغير اللون للون purple يبقى هي oxidase positive
+
+539
+00:38:32,970 --> 00:38:38,340
+بكتيريا، هنا في عندي filter paper، أنا باغمره
+
+540
+00:38:38,340 --> 00:38:42,860
+بالـ reagent، وبعدين باخد البكتيريا اللي اتحول لونها
+
+541
+00:38:42,860 --> 00:38:45,660
+لـ purple يبقى أكسيداز positive، لو ضل لون colorless
+
+542
+00:38:45,660 --> 00:38:49,600
+يبقى أكسيداز negative، هنا بشكل مباشر أجيب
+
+543
+00:38:49,600 --> 00:38:52,880
+الـ reagent وأغمره بالكولوني، إذا اتحول لون الكولوني
+
+544
+00:38:52,880 --> 00:38:54,680
+للون purple يبقى positive،
+
+545
+00:38:57,100 --> 00:39:02,100
+جبت slide، حطيت عليها اللي هو الـ filter paper، غمرت
+
+546
+00:39:02,100 --> 00:39:06,600
+الـ reagent، أنا غمرت الـ reagent بيه الـ filter، حطيت
+
+547
+00:39:06,600 --> 00:39:09,740
+الـ reagent على الـ filter paper، بعدين جبت البكتيريا
+
+548
+00:39:10,150 --> 00:39:13,750
+وشفت إذا اتحول اللون للون violet يبقى هي positive،
+
+549
+00:39:13,750 --> 00:39:18,510
+لكن لو ما اتغير يبقى هي negative، يبقى عندي أكثر من
+
+550
+00:39:18,510 --> 00:39:21,890
+طريقة للفحص، إما يكون الـ reagent، الـ reagent بشكل
+
+551
+00:39:21,890 --> 00:39:24,990
+مباشر أديرو على الـ colony، أو أجيب filter paper أغمر
+
+552
+00:39:24,990 --> 00:39:28,670
+الـ reagent أنا، بعدين أحط البكتيريا، ممكن يكون في
+
+553
+00:39:28,670 --> 00:39:33,340
+عندي اللي هو strip، هي الـ strip أو الـ slide بيكون
+
+554
+00:39:33,340 --> 00:39:36,460
+مغمورة بالأصل بالـ reagent، أنا فقط بضيف الـ colony،
+
+555
+00:39:36,460 --> 00:39:40,020
+ممكن يكون في عندي disk، الـ disk هذا برضه نفس الفكرة،
+
+556
+00:39:40,020 --> 00:39:45,540
+بيكون مغمور بالـ reagent، بمسك الـ disk بنيدل، وباخد
+
+557
+00:39:45,540 --> 00:39:49,400
+colony من الطبق، إذا اتحول لون الـ disk للون
+
+558
+00:39:49,400 --> 00:39:52,380
+violet يبقى هو positive، يبقى باختلاف الطرق إذا
+
+559
+00:39:52,380 --> 00:39:55,240
+اتحول اللون للون violet أو dark purple يبقى هي
+
+560
+00:39:55,240 --> 00:39:59,860
+positive، لكن لو كان colorless فهي negative، هيك
+
+561
+00:39:59,860 --> 00:40:03,950
+بنكون خلصنا، الـ Citrate Utilization and Oxidase
+
+562
+00:40:03,950 --> 00:40:08,710
+Test، الاثنين indole enzyme، يعطيكم العافية جميعاً
+
+563
+00:40:08,710 --> 00:40:11,350
+والسلام عليكم ورحمة الله وبركاته
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ca4bc2mptiY_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ca4bc2mptiY_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..50129ed9497c4320274483909f4db8d38c7b46ec
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ca4bc2mptiY_postprocess.srt
@@ -0,0 +1,2252 @@
+1
+00:00:02,140 --> 00:00:06,040
+السلام عليكم ورحمة الله وبركاته في هذا اللقاء
+
+2
+00:00:06,040 --> 00:00:10,080
+هتكون المحاضرة بعنوان Citrate Utilization and
+
+3
+00:00:10,080 --> 00:00:14,940
+Oxidase Production هنتكلم عن إنزيم الـ Citrate وعن
+
+4
+00:00:14,940 --> 00:00:20,140
+إنزيم الـ Oxidase وعرفنا إن هدول الإنزيمين يعتبروا
+
+5
+00:00:20,140 --> 00:00:24,920
+Endoenzyme هنبدأ بـ Citrate Utilization استهلاك
+
+6
+00:00:24,920 --> 00:00:28,380
+الـ Citrate ذكرنا سابقا أنواع الـ enzymatic
+
+7
+00:00:28,380 --> 00:00:32,390
+reactionكان من ضمنها utilization of non
+
+8
+00:00:32,390 --> 00:00:36,630
+-carbohydrate carbon source ذكرنا مثال اليوريا
+
+9
+00:00:36,630 --> 00:00:41,530
+والسيطران طيب ليش البكتيريا بدها carbon source؟
+
+10
+00:00:41,530 --> 00:00:45,570
+بقول لي البكتيريا عشان تنمو وتتكثر بحاجة ل DNA
+
+11
+00:00:45,570 --> 00:00:49,450
+replication عشان يصير بعدها transcription بعدين
+
+12
+00:00:49,450 --> 00:00:54,030
+يصير ال translation لتكوين ال protein عشان يتكون ل
+
+13
+00:00:54,030 --> 00:00:58,280
+protein فهي بحاجة ل carbonand nitrogen source
+
+14
+00:00:58,280 --> 00:01:03,660
+البكتيريا راح تستهلك مصادر للحصول عليهم بالنسبة لل
+
+15
+00:01:03,660 --> 00:01:07,540
+carbon source ممكن انها تستهلك الكربوهيدرات الشكر
+
+16
+00:01:07,540 --> 00:01:12,280
+وممكن انها تستهلك من مصادر non carbohydrate زي
+
+17
+00:01:12,280 --> 00:01:16,960
+اليوريا ال amino acid السيترات اليوم موضوعنا هيكون
+
+18
+00:01:16,960 --> 00:01:22,140
+عن استهلاك السيترات كيف البكتيريا بتستهلك السيترات
+
+19
+00:01:22,140 --> 00:01:26,550
+للحصول على ال carbon sourceبالنسبة لأ ال nitrogen
+
+20
+00:01:26,550 --> 00:01:29,590
+source في عندي ال amino acid الأمانية هي تعتبر
+
+21
+00:01:29,590 --> 00:01:34,770
+nitrogen source لازمة لنمو البكتيريا السيتريات
+
+22
+00:01:34,770 --> 00:01:38,630
+يبقى يعتبر non carbohydrate carbon source بيكون
+
+23
+00:01:38,630 --> 00:01:43,250
+على شكل sodium سيتريات في بعض أنواع البكتيريا
+
+24
+00:01:43,250 --> 00:01:47,350
+قادرة على استهلاك ال sodium سيتريات و الحصول على
+
+25
+00:01:47,350 --> 00:01:52,310
+carbonلازم لنموء البكتيريا وينقسمها لكن في بعض
+
+26
+00:01:52,310 --> 00:01:55,250
+أنواع البكتيريا ما عندها القدرة على استهلاك
+
+27
+00:01:55,250 --> 00:01:59,870
+السيترات طب كيف بتكون السيترات داخل الخلية؟ زي ما
+
+28
+00:01:59,870 --> 00:02:03,750
+قلنا في الكتاليز الـ hydrogen peroxide بتكون خلال
+
+29
+00:02:03,750 --> 00:02:09,170
+عملية التنفس الخلوي السيترات لازم يكون انتلوديات
+
+30
+00:02:09,170 --> 00:02:14,550
+في إحدى العمليات الأيضيةعشان يصلّه استهلاك بقولّي
+
+31
+00:02:14,550 --> 00:02:17,870
+أن السيطرات ال Sodium citrate بيكون intermediate
+
+32
+00:02:17,870 --> 00:02:22,970
+في ال crib cycle في ال crib cycle يبقى ال Sodium
+
+33
+00:02:22,970 --> 00:02:26,270
+citrate هو ال substrate اللي هيشتغل عليه ال enzyme
+
+34
+00:02:26,270 --> 00:02:30,910
+بيكون في ال in vivo intermediate في ال crib cycle
+
+35
+00:02:30,910 --> 00:02:36,430
+بالنسبة للتفاعل كيف هتستهلك البكتيريا ال Sodium
+
+36
+00:02:36,430 --> 00:02:40,630
+citrate بقولّي أن انزيمات السيطرات تعتبر
+
+37
+00:02:40,630 --> 00:02:45,130
+endoenzymeعشان البكتيريا تقدر تستهلك الـ Sodium
+
+38
+00:02:45,130 --> 00:02:48,830
+Citrate لازم يكون عند البكتيريا انزيم الـ Citrate
+
+39
+00:02:48,830 --> 00:02:54,870
+لازم هذا الانزيم هيكسر Sodium Citrate اللي اتكون
+
+40
+00:02:54,870 --> 00:03:00,330
+في الـ Crepe Cycle هتحول Sodium Citrate لـ
+
+41
+00:03:00,330 --> 00:03:05,100
+Oxaloacetic Acid زائد أسيتاتكمان البكتيريا اللى
+
+42
+00:03:05,100 --> 00:03:08,380
+عندها القدرة أو اللى عندها انزيم استيتريات لياز في
+
+43
+00:03:08,380 --> 00:03:12,360
+عندها انزيم الـ oxaloacetic acid decarboxylase
+
+44
+00:03:12,360 --> 00:03:17,640
+هتنزع الـ CO2 الكربون من ال oxaloacetic acid
+
+45
+00:03:17,640 --> 00:03:22,060
+هيتكون عندى بيروفات البيروفات هو ال carbon source
+
+46
+00:03:22,060 --> 00:03:27,500
+هو ال carbon source اللى لازم لنمو البكتيريا
+
+47
+00:03:27,500 --> 00:03:31,550
+وانقسمهاأحنا قلنا إن الـ oxaloacetic acid هيعمل
+
+48
+00:03:31,550 --> 00:03:35,910
+decarboxylase هينزع الـ CO2 في عندي الصوديوم
+
+49
+00:03:35,910 --> 00:03:39,210
+الصوديوم الـ excess من الصوديوم مع الـ CO2 هيتكون
+
+50
+00:03:39,210 --> 00:03:45,910
+عندي Na2CO3 هذا يعتبر alkaline فبالتالي هيرفعلي
+
+51
+00:03:45,910 --> 00:03:50,810
+الـ pH يبقى صار عندي مصدر كربون من non
+
+52
+00:03:50,810 --> 00:03:55,820
+carbohydrate carbon source من الـ citrateبقول لي
+
+53
+00:03:55,820 --> 00:03:59,200
+البكتيريا اللى عندها القدرة على استهلاك الـ Sodium
+
+54
+00:03:59,200 --> 00:04:03,420
+Citrate إلى جانب استهلاك هلاء الـ Citrate عندها
+
+55
+00:04:03,420 --> 00:04:09,020
+القدرة على استهلاك الـ Ammonia الـ NH4 Plus عندها
+
+56
+00:04:09,020 --> 00:04:14,740
+القدرة على إنتاجه وتحويله لـ Ammonia هذا يعتبر
+
+57
+00:04:14,740 --> 00:04:18,880
+Nitrogen Source يبقى صار عندي مصدر للنيتروجين
+
+58
+00:04:18,880 --> 00:04:23,160
+ومصدر للكربون هيك البكتيريا بتقدر تنمو وتنقسم
+
+59
+00:04:23,160 --> 00:04:28,930
+وتتكعفرهذا بالنسبة لـ In-Vivo طيب In-Vitro عشان
+
+60
+00:04:28,930 --> 00:04:33,870
+أكشف هل البكتيريا عندها أنزيم السيطرات لياز أو لأ
+
+61
+00:04:33,870 --> 00:04:38,790
+لازم أنا يكون في ال media Sodium Citrate هي
+
+62
+00:04:38,790 --> 00:04:42,570
+السبسترات اللي هيشتغل عليها الانزال يبقى In-Vitro
+
+63
+00:04:42,570 --> 00:04:46,750
+عشان أكشف عن وجود الانزيم بقوللي بتحط في ال media
+
+64
+00:04:46,750 --> 00:04:50,650
+Sodium Citrate السبسترال عشان يشتغل عليها الانزيم
+
+65
+00:04:50,650 --> 00:04:56,630
+هذه موجودة في ال media طيبالانزيم السيطرية تلياز
+
+66
+00:04:56,630 --> 00:05:00,370
+قلنا يعتبر اندو انزايم التفاعل بيصير intra
+
+67
+00:05:00,370 --> 00:05:03,910
+cellular داخل الخلية ال Sodium سيطرية حجمها مش
+
+68
+00:05:03,910 --> 00:05:08,850
+كبير ال substrate حجمها مش كبير بالتالي ال انزيم
+
+69
+00:05:08,850 --> 00:05:13,130
+او ال substrate بما انها موجودة في ال media يبقى
+
+70
+00:05:13,130 --> 00:05:18,090
+خارج الخلية بقولنا لازم تدخل لداخل البكتيريا احنا
+
+71
+00:05:18,090 --> 00:05:21,850
+قلنا لو كانتلو كان الانزيم اندو انزايم ممكن
+
+72
+00:05:21,850 --> 00:05:24,950
+السبسترات يكون داخل الخلية وممكن يكون خارج الخلية
+
+73
+00:05:24,950 --> 00:05:28,250
+إذا كان داخل الخلية كان بيحصل التفاعل داخل الخلية
+
+74
+00:05:28,250 --> 00:05:34,350
+لو كان خارج الخلية البكتيريا هتفرز انزيمة تدخل
+
+75
+00:05:34,350 --> 00:05:38,870
+السبسترات لداخل الخلية بقول لها البكتيريا هتفرز
+
+76
+00:05:38,870 --> 00:05:43,190
+انزيم السيترات بيريماز هذا هيدخل السوديوم سيترات
+
+77
+00:05:43,190 --> 00:05:47,870
+الموجود في الميديا لداخل الخلية تمامهكذا أصبح الـ
+
+78
+00:05:47,870 --> 00:05:52,450
+substrate موجود داخل الخلية نكمل الخطوات نفس اللي
+
+79
+00:05:52,450 --> 00:05:57,910
+ذكرناها بالـ in vivoهيجي السيطرات لياز يكسر الـ
+
+80
+00:05:57,910 --> 00:06:01,510
+Sodium سيطرات الموجود داخل الخلية سار هيكسره
+
+81
+00:06:01,510 --> 00:06:05,990
+لأجزاله Acetic acid و Acetate هيجي الأجزاله Acetic
+
+82
+00:06:05,990 --> 00:06:09,930
+acid دي كربوكسلاز هينزع الـ CO2 يتكون بيروفات هو
+
+83
+00:06:09,930 --> 00:06:14,810
+ال carbon source هيظل عندي excess of Sodium مع الـ
+
+84
+00:06:14,810 --> 00:06:19,010
+CO2 هيصير في عندي alkaline product هيرفعلي الـ pH
+
+85
+00:06:19,010 --> 00:06:22,450
+إلى جانب استهلاكها لأس Sodium سيطرات هيتم
+
+86
+00:06:22,450 --> 00:06:27,210
+استهلاكها الـ NH4 plus هتتحول لأمونيا تعتبر ك
+
+87
+00:06:27,210 --> 00:06:31,770
+نيتروجين source يبقى هاي ال principle الفحص تكون
+
+88
+00:06:31,770 --> 00:06:34,970
+عندي بالنهاية ال biofat اللي هو ال carbon source
+
+89
+00:06:34,970 --> 00:06:40,910
+والأمونيا ال nitrogen source citrate utilization
+
+90
+00:06:40,910 --> 00:06:45,970
+test for the ability of bacteria to convertCytrate
+
+91
+00:06:45,970 --> 00:06:50,890
+into oxaloacetate بقول لي هو هذا الفحص بدي أكشف عن
+
+92
+00:06:50,890 --> 00:06:54,690
+قدرة البكتيري عن تحويل الـCytrate تمام اللي هو
+
+93
+00:06:54,690 --> 00:06:57,870
+يعتبر intermediate في الـ Krebs Cycle ل
+
+94
+00:06:57,870 --> 00:07:01,450
+Oxaloacetate برضه intermediate آخر في ال Krebs
+
+95
+00:07:01,450 --> 00:07:05,260
+Cycleهيتحول لـ Oxaloacetate وبعدين التفاعل اللي
+
+96
+00:07:05,260 --> 00:07:10,540
+قلنا بيتحول لبيروفات لمصدر للـ CARB هذا الاختبار
+
+97
+00:07:10,540 --> 00:07:14,000
+استخدم للتفارق بين مجموعات العائلة الـ
+
+98
+00:07:14,000 --> 00:07:18,260
+Enterobacteraceae هذه الفقرة هي المهمة لهذا الفحص
+
+99
+00:07:18,260 --> 00:07:21,960
+هذا الفحص يفرق بين مين ومين بقول هذا الفحص يفرق
+
+100
+00:07:21,960 --> 00:07:27,200
+مجموعات الـ Enterobacteraceae it is selective for
+
+101
+00:07:27,200 --> 00:07:31,570
+bacteria that hasthe ability to consume citrate as
+
+102
+00:07:31,570 --> 00:07:34,850
+its sole source of the carbon بقولّي هذه ال media
+
+103
+00:07:34,850 --> 00:07:38,830
+ال citrate هيتعرف عليها كمان شوية هي selective
+
+104
+00:07:38,830 --> 00:07:44,450
+media اتعرفنا قبل في المعمل السابق على أنواع ال
+
+105
+00:07:44,450 --> 00:07:47,970
+media حسب ال function قسمناها إلى general
+
+106
+00:07:47,970 --> 00:07:51,670
+selective differential transport ال selective قلنا
+
+107
+00:07:51,670 --> 00:07:56,650
+بتنمي نوع معين من البكتيريا ال media ال citrate
+
+108
+00:07:56,650 --> 00:08:00,460
+فيها فقطالسيطرات كمصدر
+
+109
+00:08:28,880 --> 00:08:34,600
+استهلاك السيطرات هتكسر السيطرات هتحصل على بيروفيت
+
+110
+00:08:34,600 --> 00:08:37,420
+هيكون هو ال carbon source اللازم لنمو البكتيريا
+
+111
+00:08:37,420 --> 00:08:41,360
+يبقى اللي فقط هتنمو على هذه ال media هي اللي عندها
+
+112
+00:08:41,360 --> 00:08:44,660
+القدرة على استهلاك السيطرات اللي ماعندها القدرة
+
+113
+00:08:44,660 --> 00:08:47,780
+على استهلاك السيطرات اللي ماعندها انتمات السيطرات
+
+114
+00:08:47,780 --> 00:08:51,560
+لياز ماهتنمو في هذه ال media عشان هيك قال لي
+
+115
+00:08:51,560 --> 00:08:55,720
+selective some intestinal bacteria are positive
+
+116
+00:08:55,720 --> 00:09:00,570
+سيطرات بقول لي بعضالبكتيريا الانتستينال بتكون
+
+117
+00:09:00,570 --> 00:09:03,730
+positive لهذا الفحص مثلًا كالبسيلة، الستروبكتر،
+
+118
+00:09:03,730 --> 00:09:08,110
+الانتيروبكتر لكن ال negative اللي هي ال E coli
+
+119
+00:09:08,110 --> 00:09:14,190
+يبقى هذا هو ال significant لهذا الفحص خلّينا بنشوف
+
+120
+00:09:14,190 --> 00:09:19,520
+هذا ال significant لهذا الفحصبقولنا لو عملنا
+
+121
+00:09:19,520 --> 00:09:22,480
+جرامستان و طلعت جرام نيجاتيب بس ال life يعني دي
+
+122
+00:09:22,480 --> 00:09:26,620
+أكتر من احتمال ممكن يكون صدوموناس انتيرو بكترياسي
+
+123
+00:09:26,620 --> 00:09:33,440
+فيبريو كوليرة أسينيتوباكتر هنتعرف في الفحص
+
+124
+00:09:33,440 --> 00:09:37,340
+الأكسداس كيف نفرق منهم هلأ لو طلعت البكتيريا
+
+125
+00:09:37,340 --> 00:09:41,060
+انتيرو بكترياسي بقولي انتيرو بكترياسي تقسم إلى
+
+126
+00:09:41,060 --> 00:09:45,850
+مجموعتينالكوليفارم أو بسميها ال lactose fermenter
+
+127
+00:09:45,850 --> 00:09:51,050
+الكوليفارم هي بكتيريا موجودة في الأمعاء ال non
+
+128
+00:09:51,050 --> 00:09:55,170
+-coliform أو ال non-lactose fermenter بس المجموعة
+
+129
+00:09:55,170 --> 00:09:58,110
+التانية ال non-coliform أو بسميها ال non-lactose
+
+130
+00:09:58,110 --> 00:10:02,210
+fermenter نيجي لمجموعة الكوليفارم اللاكتوز فرمنتر
+
+131
+00:10:02,210 --> 00:10:07,150
+بقول يتقسم برضه لاسمين فيكل اللي هي مصدرها ال
+
+132
+00:10:07,150 --> 00:10:09,410
+stool الفيكل
+
+133
+00:10:11,230 --> 00:10:14,590
+كوليفارم اللي هي الـ E coli و الـ Klebsiella لكن
+
+134
+00:10:14,590 --> 00:10:17,530
+الـ non-fecal اللي هي مصدرها مش الستول زي ورق
+
+135
+00:10:17,530 --> 00:10:21,570
+الشجر الـ Enterobacter و الستروبكتر لو عملت فحص
+
+136
+00:10:21,570 --> 00:10:26,530
+السيطرات و لفحص السيطرات في الكوليفارم الـ E coli
+
+137
+00:10:26,530 --> 00:10:30,450
+negative لكن باقي ال Klebsiella ال Enterobacter و
+
+138
+00:10:30,450 --> 00:10:35,270
+الستروبكتر يعتبروا positive في فحص السيطرات ذكرنا
+
+139
+00:10:35,270 --> 00:10:41,090
+سابقا فحص ال motilityكلهم motile معادلة الكيبسيلا
+
+140
+00:10:41,090 --> 00:10:44,870
+non motile يبقى عندي فحصين للتفريق بين الكوليفار
+
+141
+00:10:44,870 --> 00:10:47,970
+من ال lactose fermenter السيطرات و ال motility
+
+142
+00:10:47,970 --> 00:10:51,570
+عشان
+
+143
+00:10:51,570 --> 00:10:56,670
+أكشف عن هل البكتيري عندها أنزيم السيطرات لياز أو
+
+144
+00:10:56,670 --> 00:11:00,330
+لأ عندها القدرة على استهلاك السيطرات ولا لأ بقولي
+
+145
+00:11:00,330 --> 00:11:04,810
+في عندي طريقتين الطريقة الأولى اسمها Simons method
+
+146
+00:11:04,810 --> 00:11:09,930
+الطريقة التانية اسمها Causars methodالسيمونز ميثود
+
+147
+00:11:09,930 --> 00:11:14,550
+بقولي بستخدم ميديا اسمها simon citrate agar هي
+
+148
+00:11:14,550 --> 00:11:20,030
+الميديا انا بحضرها بعد ما انا احضرها ب .. بعمل ..
+
+149
+00:11:20,030 --> 00:11:24,690
+ب .. دوبها على ال hot plate بعد ما الدوب بصوبها في
+
+150
+00:11:24,690 --> 00:11:27,990
+تيوب بدخلها ال autoclave تتعقم بعد ما تطلع من ال
+
+151
+00:11:27,990 --> 00:11:32,090
+autoclave ال autoclave بدي اخليها تتصلب بزاوية
+
+152
+00:11:32,090 --> 00:11:36,670
+بديها تكون slant تمام؟بعد ما تتصلب في زاوية بتكون
+
+153
+00:11:36,670 --> 00:11:41,470
+جاهزة للاستخدام بقول لي عشان تكشفي عن قدرة
+
+154
+00:11:41,470 --> 00:11:45,010
+البكتيريا عن استهلاك السايتريات أو لأ بتجيبي
+
+155
+00:11:45,010 --> 00:11:49,370
+السيمون سايتريات أجار وبتاخدي loop full of بكتيريا
+
+156
+00:11:49,370 --> 00:11:53,910
+بتعملي كيف أنا بزرع ال slug بعمل stabbing بعدين
+
+157
+00:11:53,910 --> 00:11:58,090
+بزرع دي جزارة بعد ما أزرع البكتيريا اللي بدي أكشف
+
+158
+00:11:58,090 --> 00:12:01,290
+قدرتها على استهلاك السايتريات أو لأ بعمل
+
+159
+00:12:01,290 --> 00:12:08,240
+incubation لمدة 24 ساعة37 درجة سليسيا صح بقول لي
+
+160
+00:12:08,240 --> 00:12:13,380
+لو نمت البكتيريا واتحول اللون للون الأزرق يبقى هي
+
+161
+00:12:13,380 --> 00:12:17,400
+positive للفحص لكن لو ما نمت البكتيريا واتحول
+
+162
+00:12:17,400 --> 00:12:21,060
+اللون لللون أو ضلت ال media لونها أخضر لون ال
+
+163
+00:12:21,060 --> 00:12:24,360
+media لونها أخضر لو ضل ال media لونها أخضر يبقى هي
+
+164
+00:12:24,360 --> 00:12:29,080
+negative لهذا الفحص عشان نفهم أكترليش اتحول الـ
+
+165
+00:12:29,080 --> 00:12:32,180
+Loan و ليش في عندي Growth في النتيجة الـ Positive
+
+166
+00:12:32,180 --> 00:12:36,220
+بإننا نتعرف على مكونات الـ Media بما إنها أجار
+
+167
+00:12:36,220 --> 00:12:40,820
+بقولنا نسبة الأجار فيها بيكون 1.5% في عندي Sodium
+
+168
+00:12:40,820 --> 00:12:45,180
+Citrate و NH4 Plus كSubstrate اللي هيشتغل عليهم
+
+169
+00:12:45,180 --> 00:12:49,320
+الانزيم الـ Citrate عشان أحصل على Carbon و
+
+170
+00:12:49,320 --> 00:12:53,060
+Nitrogen Source مافي عندي أي مصدر آخر للكربون
+
+171
+00:12:53,060 --> 00:12:55,740
+والنيترجن مافي عندي Beef Extract ولا بيبتون
+
+172
+00:12:55,740 --> 00:13:00,420
+الموجودين في أي Media فقطموجود Sodium Citrate و N
+
+173
+00:13:00,420 --> 00:13:04,480
+-H4 Plus هما اللي هيكونوا المصدر الوحيد لل Carbon
+
+174
+00:13:04,480 --> 00:13:08,920
+و ال Nitrogen NACL اللي هو ال Normal Saline أي
+
+175
+00:13:08,920 --> 00:13:13,120
+ميديا هيكون فيها ال NACL عشان تحافظ على ال Osmotic
+
+176
+00:13:13,120 --> 00:13:17,760
+إبرادشار فهذه الميديا فيها عندى BH Indicator ال BH
+
+177
+00:13:17,760 --> 00:13:23,220
+Indicator اسمه Bromothymol Blue عشان أكشف صار فيا
+
+178
+00:13:23,220 --> 00:13:27,660
+عندى التفاعل ولا لأالبرامو ثيمال بلو حدوده ان عندي
+
+179
+00:13:27,660 --> 00:13:32,360
+انا من ال 96 ل 76 بيكون ال neutral بيكون اللون
+
+180
+00:13:32,360 --> 00:13:37,240
+اللون إجرين يبقى
+
+181
+00:13:37,240 --> 00:13:42,900
+لما يكون ال bh ما بين 96 و 76 بيكون اللون ال media
+
+182
+00:13:42,900 --> 00:13:47,800
+إجرين بقول لو كان أقل من 96 ال bh بيكون بتحول
+
+183
+00:13:47,800 --> 00:13:51,900
+اللون للون yellow لو كان أعلى من 76 بيكون ال
+
+184
+00:13:51,900 --> 00:13:57,040
+alkalineبتحول اللون للون e-blue احنا قولنا لو
+
+185
+00:13:57,040 --> 00:14:02,120
+البكتيريا عملت استهلاك لل Sodium citrate Sodium
+
+186
+00:14:02,120 --> 00:14:07,390
+citrate هو المصدر الوحيد لل carbon فتستهلكهتعمل
+
+187
+00:14:07,390 --> 00:14:11,750
+تكسير استهلاك للسيدرات هيتحول السيدرات ل biofat ال
+
+188
+00:14:11,750 --> 00:14:15,870
+biofat هو المصدر الوحيد لقى الكربون إلى جانب
+
+189
+00:14:15,870 --> 00:14:19,830
+استهلاك هلاقى ال sodium سيدرات هتستهلك ال N-H4
+
+190
+00:14:19,830 --> 00:14:24,450
+plus هتحوله ل أمونيا هيعتبر ك nitrogen source
+
+191
+00:14:24,450 --> 00:14:27,690
+فبالتالي صار عندى carbon source و nitrogen source
+
+192
+00:14:27,690 --> 00:14:33,090
+هتنمو البكتيريا و تتكاثر طبعا انا عندى اتكوين
+
+193
+00:14:33,090 --> 00:14:38,480
+الأمونيا ال N-Hالـ NH3 يعتبر alkaline فبالتالي
+
+194
+00:14:38,480 --> 00:14:43,560
+هيرفع لي ال BH ويتحول لون ال media للون blue احنا
+
+195
+00:14:43,560 --> 00:14:46,840
+قلنا ان لون ال media green في الأصل قبل ما انا
+
+196
+00:14:46,840 --> 00:14:52,540
+ازرع صار في عندي ارتفاع في ال BH صار ال BH عندي
+
+197
+00:14:52,540 --> 00:14:59,500
+alkaline يتحول لون ال media للون blueهذا المخطط
+
+198
+00:14:59,500 --> 00:15:04,260
+يبقى هيكون في عندى نمو للبكتيريا لأنه في عندها صار
+
+199
+00:15:04,260 --> 00:15:08,480
+مصدر لل carbon ومصدر لل nitrogen اللي هم ال sodium
+
+200
+00:15:08,480 --> 00:15:13,800
+citrate و ال N-H4 plus اتحول لوان الوسط للوان blue
+
+201
+00:15:13,800 --> 00:15:16,740
+لأنه اتكون عندى ال ammonia ال ammonia تعتبر
+
+202
+00:15:16,740 --> 00:15:19,980
+alkaline كمان قلنا في تفاعل ال sodium citrate
+
+203
+00:15:19,980 --> 00:15:24,490
+اتكون عندىبالتالي إكسس من النا من الصوديوم مع ال
+
+204
+00:15:24,490 --> 00:15:30,490
+CO2 بتكون عندي NA2CO3 هذا ال alkaline فبالتالي
+
+205
+00:15:30,490 --> 00:15:35,590
+برضه هيرفعلي ال pH لكن لو البكتيريا ما عندها انزيم
+
+206
+00:15:35,590 --> 00:15:39,190
+السيترات لياز أو ماعندها القدرة على استهلاك
+
+207
+00:15:39,190 --> 00:15:39,970
+السيترات
+
+208
+00:15:42,250 --> 00:15:45,950
+البكتيريا ماعندها كمان مصادر اخرى لكربون او
+
+209
+00:15:45,950 --> 00:15:49,010
+نيتروجين مصادر اخرى مافي عندى في ال media اي مصادر
+
+210
+00:15:49,010 --> 00:15:53,050
+اخرى مافي عندى beef extract ولا ببطون فبالتالي
+
+211
+00:15:53,050 --> 00:15:57,640
+البكتيريا مش هتنمو مش هيكون في عندي growthالـ ما
+
+212
+00:15:57,640 --> 00:16:01,420
+تكون عندي أي product alkaline يبقى يظل الوسط
+
+213
+00:16:01,420 --> 00:16:05,020
+agreed فيه لـ neutral يبقى هذا تفسير النتائج في
+
+214
+00:16:05,020 --> 00:16:08,380
+الـ positive بكون عندي growth زائد اللون اللون
+
+215
+00:16:08,380 --> 00:16:13,400
+blue لكن في الـ negative no growth مع بقاء ال
+
+216
+00:16:13,400 --> 00:16:18,280
+media لونها agreed بقولي لو صار عندي لون أزرقلكن
+
+217
+00:16:18,280 --> 00:16:22,800
+مع وجود no growth مافي عندى اى نمو بقوللى هان بكون
+
+218
+00:16:22,800 --> 00:16:26,640
+خلل فى ال media سواء فى التخزين او انها انتهت فترة
+
+219
+00:16:26,640 --> 00:16:31,780
+صلاحيتها هذا بالنسبة ل ال Simon method اللى بستخدم
+
+220
+00:16:31,780 --> 00:16:35,280
+فيها Simon سيطرات اجار بالنسبة لطريقة ال Quasar
+
+221
+00:16:35,280 --> 00:16:40,440
+method بستخدم Quasar سيطرات بروث و ليس اجار بقوللى
+
+222
+00:16:41,720 --> 00:16:45,340
+هذه ال media بتكون ال broth بحضرها عادي بتكون
+
+223
+00:16:45,340 --> 00:16:49,520
+موجودة بوتيل بحضرها ال broth سائلة بجيب loop full
+
+224
+00:16:49,520 --> 00:16:53,420
+of ال bacteria البكتيريا اللي بتكشف عندها انزيم
+
+225
+00:16:53,420 --> 00:16:56,080
+السيطرات لياز أو لأ انها القدرة على استهلاك
+
+226
+00:16:56,080 --> 00:16:59,780
+السيطرات أو لأ بعمل mix بعمل incubation لمدة 24
+
+227
+00:16:59,780 --> 00:17:04,960
+ساعة على 37 درجة سيليزياإذا كان في عندي نمو يبقى
+
+228
+00:17:04,960 --> 00:17:10,480
+هي positive للفحص إذا مافي عندي نمو يبقى فيه مافي
+
+229
+00:17:10,480 --> 00:17:15,620
+عندي نمو يبقى negative للفحص بالنسبة للنتيجة ال
+
+230
+00:17:15,620 --> 00:17:19,440
+growth ال growth في ال broth قولنا إذا بتتذكروا في
+
+231
+00:17:19,440 --> 00:17:23,180
+معمل ال media preparation قولنا أنواع النمو في ال
+
+232
+00:17:23,180 --> 00:17:29,040
+broth ممكن يكون turbidity أو ممكن يكون pellicle أو
+
+233
+00:17:29,040 --> 00:17:34,070
+ممكن يكون sedimentفبالتالي أي شكل من هذه الأشكال
+
+234
+00:17:34,070 --> 00:17:39,290
+يعتبر أنه في عندي Growth هذا أشكال أنه مافي البروث
+
+235
+00:17:39,290 --> 00:17:42,810
+EQ مكونات ال media بما أنها البروث مافي عندي أجار
+
+236
+00:17:42,810 --> 00:17:47,630
+في عندي Sodium Citrate و NH4 Plus كsubstrate هذه
+
+237
+00:17:47,630 --> 00:17:51,110
+هتكون مصدر للكربون مصدر وحيد للكربون هذه هتكون
+
+238
+00:17:51,110 --> 00:17:56,310
+مصدر وحيد للنيتروجين NACL الـ Normal Saline هو
+
+239
+00:17:57,380 --> 00:18:00,420
+هيحفظلي على الـ Osmotic Pressure مافي عندي BH
+
+240
+00:18:00,420 --> 00:18:05,080
+Indicator في هذه الميديا فبالتالي ما هيكون في عندى
+
+241
+00:18:05,080 --> 00:18:08,900
+اختلاف في اللون فقط إذا وجود النموم هو اللي
+
+242
+00:18:08,900 --> 00:18:14,330
+هيكشفلي عن هذا الانزيميبقى في عندي فروقات بين
+
+243
+00:18:14,330 --> 00:18:18,490
+الطريقتين أول فرق أن هاي الطريقة فيها أجار لكن هاي
+
+244
+00:18:18,490 --> 00:18:22,630
+الطريقة هاي ال media ال causer سيطرات هي ال growth
+
+245
+00:18:22,630 --> 00:18:28,070
+الاختلاف التاني أن هان في عندي pH indicator هان
+
+246
+00:18:28,070 --> 00:18:31,970
+مافي عندي pH indicator هان ال result هيكون في عندي
+
+247
+00:18:31,970 --> 00:18:37,260
+نمو بعد تغير اللون لكن هان فقط هيكون نموطبعاً
+
+248
+00:18:37,260 --> 00:18:42,380
+هتنمو البكتيريا لأنه لو كان عندها أنزيمات أو لو
+
+249
+00:18:42,380 --> 00:18:46,200
+كان عندها القدرة على استهلاك السييتريات هتحول الـ
+
+250
+00:18:46,200 --> 00:18:53,140
+Sodium هتكسر السييتريات لـ Oxaloacetic Acid هيتحول
+
+251
+00:18:53,140 --> 00:18:56,680
+بواسطة أنزيم الـ Oxaloacetic Acid D-Carboxylase
+
+252
+00:18:56,680 --> 00:19:00,780
+لبيهروفات مصدر للكربون لازم لنمو البكتيريا
+
+253
+00:19:00,780 --> 00:19:04,560
+بالإضافة لاستهلاكها للسييتريات هتستهلك الـ N-H4
+
+254
+00:19:04,560 --> 00:19:08,910
+Plus لأمونيافبالتالي صار في عندي مصدر للـ carbon و
+
+255
+00:19:08,910 --> 00:19:13,470
+الـ nitrogen هتنمو البكتيريا و تتكاثر و يكون في
+
+256
+00:19:13,470 --> 00:19:17,710
+عندي turbidity او sediment او pellicle لكن لو كان
+
+257
+00:19:17,710 --> 00:19:20,950
+ما عندها مش هيكون في عندي اي نمو لأن مافي عندي اي
+
+258
+00:19:20,950 --> 00:19:25,370
+مصادر اخرى لل carbon او ال nitrogen هذا بالنسبة
+
+259
+00:19:25,370 --> 00:19:31,590
+لطريقة الكشف عن السيطرات utilization نيجي لالسيمون
+
+260
+00:19:31,590 --> 00:19:35,290
+سايتريات أجار organism that metabolize citrate
+
+261
+00:19:35,290 --> 00:19:38,430
+utilize the ammonium salt releasing ammonia and
+
+262
+00:19:38,430 --> 00:19:42,030
+increasing the pH of the media بقوللي البكتيريا
+
+263
+00:19:42,030 --> 00:19:45,050
+اللي عندها القدرة على استهلاك السايتريات يعني ده
+
+264
+00:19:45,050 --> 00:19:47,850
+القدرة على استهلاك ال ammonium salt اللي هي ال N
+
+265
+00:19:47,850 --> 00:19:51,630
+-H4 plus بالتالي لما تستهلك ال ammonium salt
+
+266
+00:19:51,630 --> 00:19:56,130
+هتكوني الأمانيا الأمانيا هترفعلي ال pH في الميديا
+
+267
+00:19:56,130 --> 00:20:00,120
+في انديبرامو تيمولي بلوموجود في الـ media as
+
+268
+00:20:00,120 --> 00:20:05,080
+indicator زي BH indicator بقول هيحول اللون لـ
+
+269
+00:20:05,080 --> 00:20:10,080
+green لـ neutral BH قولنا إنه اللي فيه ال neutral
+
+270
+00:20:10,080 --> 00:20:15,640
+BH هيكون اللون إجريه بما إن ال BH ارتفع بالتالي
+
+271
+00:20:15,640 --> 00:20:20,760
+هيتحول اللون للون أزرق blue لما يكون أعلى من 7.6
+
+272
+00:20:21,600 --> 00:20:24,960
+سيمون سايتريات أجار بيعرف لسيمون سايتريات أجار
+
+273
+00:20:24,960 --> 00:20:27,940
+بتحتوي على صوديوم سايتريات as a sole carbon source
+
+274
+00:20:27,940 --> 00:20:31,440
+كمصدر واحد للكربون امونيوم ايون كمصدر واحد
+
+275
+00:20:31,440 --> 00:20:35,620
+للنيتروجين and برامو ثمال بلو لو ما كتبت برامو
+
+276
+00:20:35,620 --> 00:20:39,940
+ثمال بلو بتتحول ال media لcausar citrate broth
+
+277
+00:20:39,940 --> 00:20:44,120
+يبقى هذه بتحتوي على مصدر كربون واحد مصدر نيتروجين
+
+278
+00:20:44,120 --> 00:20:49,320
+واحد وبرامو ثمال بلوكيف طريقة الشغل قلنا نستخدم
+
+279
+00:20:49,320 --> 00:20:53,080
+eniculating needle عشان نعمل stabbing بعدين بعمل
+
+280
+00:20:53,080 --> 00:21:01,160
+zigzag بزرع ال slant بيعمل incubation لمدة 24 ساعة
+
+281
+00:21:01,160 --> 00:21:05,920
+على 37 درجة السليسيس بشوف ال growth و التغير في
+
+282
+00:21:05,920 --> 00:21:10,280
+اللون النتيجة ال positive بشوفها بكون في عندي no
+
+283
+00:21:10,280 --> 00:21:14,040
+moreمع تغير في اللون لللون الأزرق والـ growth on
+
+284
+00:21:14,040 --> 00:21:17,820
+the media is combined by a rise in a pH to change
+
+285
+00:21:17,820 --> 00:21:20,800
+the media from its initial green color to deep
+
+286
+00:21:20,800 --> 00:21:25,180
+blue والـ growth هيكون في الميديا وكمان هيكون الـ
+
+287
+00:21:25,180 --> 00:21:30,940
+pH هيتغير في الميديا من green ل blue نلاحظ هنا في
+
+288
+00:21:30,940 --> 00:21:34,620
+عندي control negative control لون الميديا ضاله لون
+
+289
+00:21:34,620 --> 00:21:41,000
+green أخضرالأزرق هنا عندي الـ Enterobacter الـ
+
+290
+00:21:41,000 --> 00:21:44,100
+Enterobacter قولنا positive للسيطرات يبقى هيتحول
+
+291
+00:21:44,100 --> 00:21:47,800
+اللون للون أزرق Equally قولنا negative يبقى هيدل
+
+292
+00:21:47,800 --> 00:21:51,100
+اللون green Eclipsella قولنا positive للسيطرات
+
+293
+00:21:51,100 --> 00:21:55,510
+يبقى هيتحول اللون للون blueالطريقة التانية الـ
+
+294
+00:21:55,510 --> 00:21:59,710
+Causar citrate broth is another medium used to
+
+295
+00:21:59,710 --> 00:22:03,270
+test for citrate utilization طريقة أخرى للكشف على
+
+296
+00:22:03,270 --> 00:22:06,810
+ال citrate utilization Causar citrate broth قلنا
+
+297
+00:22:06,810 --> 00:22:10,690
+is based on this formulation and contains Sodium
+
+298
+00:22:10,690 --> 00:22:13,630
+citrate as a single source of carbon بتحتوي على
+
+299
+00:22:13,630 --> 00:22:17,690
+Sodium citrate كمصدر وحيد للكربون Ammonium salt
+
+300
+00:22:17,930 --> 00:22:21,470
+كمصدر وحيد للنيتروجين مش موجود فيه عندي BH
+
+301
+00:22:21,470 --> 00:22:24,930
+Indicator يبقى هذه الـ Causar Citrate البروث
+
+302
+00:22:24,930 --> 00:22:28,490
+بكتيريا قادرة على استخدام الـ Citrate كمصدر كربون
+
+303
+00:22:28,490 --> 00:22:32,110
+سارس ستنمو في الميديا ويسبب تربيدتي بقول البكتيريا
+
+304
+00:22:32,110 --> 00:22:35,190
+اللي عندها القدرة على استهلاك الـ Citrate كمصدر
+
+305
+00:22:35,190 --> 00:22:38,410
+وحيد للكربون هتنمو في الميديا وهذا هيسبب تربيدتي
+
+306
+00:22:38,410 --> 00:22:41,670
+قلنا مش بس تربيدتي ممكن يكون sediment وممكن يكون
+
+307
+00:22:41,670 --> 00:22:46,550
+pellicle لأنه أشكال النمو في البروث تيوب تربيدتي
+
+308
+00:22:46,550 --> 00:22:51,030
+بيليكل سيدمانالنتيجة الـ Positive Growth كافة ان
+
+309
+00:22:51,030 --> 00:22:54,930
+مو تربدتي زائد sediment زائد pellicle الـ Negative
+
+310
+00:22:54,930 --> 00:22:59,090
+No growth هتكون clear مافي عندي اي تغير في اللون
+
+311
+00:22:59,090 --> 00:23:04,410
+خلصنا من ال citrate utilization يُعتبر endoenzyme
+
+312
+00:23:04,410 --> 00:23:09,210
+نيجي لل Oxidase test ال Oxidase test يُعتبر أو ال
+
+313
+00:23:09,210 --> 00:23:13,780
+Oxidase enzyme أيضا يُعتبر endoenzymeبقول لي the
+
+314
+00:23:13,780 --> 00:23:16,940
+oxidase test is used to identify bacteria
+
+315
+00:23:16,940 --> 00:23:20,220
+containing the respiratory enzyme cytochrome-c
+
+316
+00:23:20,220 --> 00:23:24,280
+oxidase يبقى بدي أكشف هل عند البكتيريا إنزيم الـ
+
+317
+00:23:24,280 --> 00:23:27,940
+cytochrome-c أكسداز ولا لأ اسمه هك لأن ال
+
+318
+00:23:27,940 --> 00:23:30,220
+substrate اللي هيشتغل عليها ال enzyme هي ال
+
+319
+00:23:30,220 --> 00:23:34,480
+cytochrome-c يبقى ال substrate ال cytochrome-c
+
+320
+00:23:34,480 --> 00:23:39,000
+الإنزيم اللي بدي أكشف عليه أكسدازكيف بتكون الـ
+
+321
+00:23:39,000 --> 00:23:42,460
+cytochrome-C؟ بقول الـ cytochrome-C أكسيداز is the
+
+322
+00:23:42,460 --> 00:23:45,540
+terminal enzyme in the electron transport chain
+
+323
+00:23:45,540 --> 00:23:48,780
+بقول لي هو الانزيم النهائي في ال electron
+
+324
+00:23:48,780 --> 00:23:52,960
+transport chain the enzyme which reduce molecular
+
+325
+00:23:52,960 --> 00:23:56,080
+oxygen to water to complete the last link in the
+
+326
+00:23:56,080 --> 00:24:01,480
+chain of aerobic respirationبتكون في نهاية اللي هو
+
+327
+00:24:01,480 --> 00:24:06,600
+عملية التنفس الخلوي في نهاية الـ Krebs Cycle بيحدث
+
+328
+00:24:06,600 --> 00:24:09,900
+Redox Reaction اللي هو Oxidation Reduction
+
+329
+00:24:09,900 --> 00:24:14,780
+Reaction الـ Cytochrome C أكسيداز موجود في الـ
+
+330
+00:24:14,780 --> 00:24:17,500
+Electron Transport Exchange هذا الإنزين بيقول
+
+331
+00:24:17,500 --> 00:24:23,190
+بيعمل Reduce لـ Molecular Oxygen لوطةعشان نفهم
+
+332
+00:24:23,190 --> 00:24:26,590
+أكتر قولنا الـ cytochrome-C أكسداز بيشتغل على الـ
+
+333
+00:24:26,590 --> 00:24:29,510
+substrates اللي هي الـ cytochrome-C أو بسميها
+
+334
+00:24:29,510 --> 00:24:34,670
+reduced cytochrome-C اش يعني reduced يعني مختزل
+
+335
+00:24:34,670 --> 00:24:38,270
+يعني مقتسب إلكترونات الـ cytochrome-C أكسداز من
+
+336
+00:24:38,270 --> 00:24:43,470
+اسمه هيعمل oxidation ل الـ reduced cytochrome-C
+
+337
+00:24:43,470 --> 00:24:47,730
+كانت مكتسبة إلكترونات هتصير فاقدة للإلكترونات
+
+338
+00:24:47,730 --> 00:24:52,920
+هتتحول لأكسداز cytochrome-Cبما أنها فقدت إلكترون
+
+339
+00:24:52,920 --> 00:24:57,720
+يبقى الإلكترون هيروح لإيش تاني لمركب تاني
+
+340
+00:24:57,720 --> 00:25:02,640
+الإلكترون اللي فقدته بقولي هتاخده الـ O2 الـ
+
+341
+00:25:02,640 --> 00:25:06,940
+Molecular Oxygen الـ O2 مع الـ H Plus قولنا في
+
+342
+00:25:06,940 --> 00:25:10,680
+عندي مضخات لترانسبورت الإلكترون الترانسبورت تتشهد
+
+343
+00:25:10,680 --> 00:25:15,880
+مضخات للـ H Plus يبقى عندي H Plus مع الـ Molecular
+
+344
+00:25:15,880 --> 00:25:21,590
+Oxygenفي عندي إلكترون تم فقده خلال عملية الـ
+
+345
+00:25:21,590 --> 00:25:25,410
+Oxidation لـ Cytochrome-C لـ Reduce Cytochrome-C
+
+346
+00:25:25,410 --> 00:25:31,570
+إلكترون زائد H بلس زائد O2 هيتكون عندي H2O يبقى
+
+347
+00:25:31,570 --> 00:25:37,930
+هذا هو ال issue اللي بيصير in vivo in vivo بقولي
+
+348
+00:25:37,930 --> 00:25:42,690
+إن Cytochrome-C Oxidase هيعمل Oxidation لـ Reduce
+
+349
+00:25:42,690 --> 00:25:47,440
+Cytochrome-CReduce يعني مختزل يعني مكتز بإلكترونات
+
+350
+00:25:47,440 --> 00:25:50,640
+بما أنه صار له أكسدة يبقى فقد الإلكترونات صار
+
+351
+00:25:50,640 --> 00:25:54,960
+Oxidized Cytochrome-C الإلكترون وين هيروح؟
+
+352
+00:25:54,960 --> 00:25:59,100
+الإلكترون بقولّي هيرتبط مع الـ Molecular Oxygen مع
+
+353
+00:25:59,100 --> 00:26:02,080
+الـH Plus اللي هي موجود في عندي مدخّات في
+
+354
+00:26:02,080 --> 00:26:04,880
+الإلكترون Transporter تشين هيرتبطوا مع بعض
+
+355
+00:26:04,880 --> 00:26:10,080
+ويكونوني الـH2O يبقى هيك صار في عنده التفاعل in
+
+356
+00:26:10,080 --> 00:26:11,680
+vivo طيب
+
+357
+00:26:19,790 --> 00:26:23,870
+عشان انا اكشف هل عندي انزيم الـ Oxidase ولا لأ
+
+358
+00:26:23,870 --> 00:26:28,570
+بقوللي بجيب Substrate بتشبه الـ Cytochrome-C والله
+
+359
+00:26:28,570 --> 00:26:32,630
+لو عندي انزيم ال Oxidase موجود في البكتيريا هتعمل
+
+360
+00:26:32,630 --> 00:26:37,150
+Oxidation للـ Cytochrome-C هتتحول ل Oxidase
+
+361
+00:26:37,150 --> 00:26:41,170
+Cytochrome-C هتتحول للون تاني بقدر اني انا اكشف
+
+362
+00:26:41,170 --> 00:26:45,910
+عنه هنتعرف هلأ في الـ Principle بقوللي Oxidase
+
+363
+00:26:45,910 --> 00:26:50,970
+producing bacteriaالأمثلة على البكتيريا Oxidase
+
+364
+00:26:50,970 --> 00:26:54,770
+Positive Sodomonas Neisseria Vibrio Aeromonas
+
+365
+00:26:54,770 --> 00:26:59,270
+Sodomonas aeruginosa is a gram negative بكتيريا
+
+366
+00:26:59,270 --> 00:27:02,270
+Sodomonas aeruginosa هي gram negative بس الله
+
+367
+00:27:02,270 --> 00:27:07,450
+aerobic rod هي aerobic هوائية have a strictly
+
+368
+00:27:07,450 --> 00:27:11,410
+respiratory type of metabolismwith oxygen as the
+
+369
+00:27:11,410 --> 00:27:14,370
+terminal electron acceptor and this is أكسداز
+
+370
+00:27:14,370 --> 00:27:17,990
+positive بقوللي إن صلمانوس إيروجينازا هي أكسداز
+
+371
+00:27:17,990 --> 00:27:22,210
+positive طالما أكسداز positive طبيعي إنها تكون
+
+372
+00:27:22,210 --> 00:27:25,870
+obligate aerobic هوائية إجبارية هوائية إجبارية
+
+373
+00:27:25,870 --> 00:27:30,930
+يعني تستخدم الأكسجين كمستقبل نهائي للإلكترونات
+
+374
+00:27:30,930 --> 00:27:39,100
+بالتالي هيصله هيكتسب إلكترون يتحول لH2O هيصيرأكسدة
+
+375
+00:27:39,100 --> 00:27:43,060
+للـ «cytochrome-c» ويتحول لأكسداء الـ «cytochrome
+
+376
+00:27:43,060 --> 00:27:48,560
+-c» لكن الأكسجن هيصله اختزال وهيكتسب إلكترون
+
+377
+00:27:48,560 --> 00:27:53,360
+وهيتحول لـH2Oالانتيرو بكترياسي بقول لي is Oxidase
+
+378
+00:27:53,360 --> 00:27:57,180
+negative bacteria يبقى هي Oxidase negative لكن الـ
+
+379
+00:27:57,180 --> 00:28:01,100
+Sodomonas هي Oxidase positive عشان أفرق الانتيرو
+
+380
+00:28:01,100 --> 00:28:04,040
+بكترياسي عن ال Sodomonas بعمل فحص ال Oxidase لو
+
+381
+00:28:04,040 --> 00:28:07,380
+طلعت positive التنتين طبعا هم إجرام نيجاتيف بصلية
+
+382
+00:28:07,380 --> 00:28:10,760
+Sodomonas و الانتيرو بكترياسي إجرام نيجاتيف بصلية
+
+383
+00:28:11,040 --> 00:28:14,140
+عشان أفرق بينهم بعمل فحص الـ Oxidase إذا طلعت
+
+384
+00:28:14,140 --> 00:28:17,100
+Negative يبقى هي Antirobacteriasy إذا طلعت
+
+385
+00:28:17,100 --> 00:28:21,120
+Positive يبقى هي Sodomonas في فحص تاني اللي هو ال
+
+386
+00:28:21,120 --> 00:28:24,860
+Gelicose Fermenter هنتعرف عليه في ال chapter الجاي
+
+387
+00:28:24,860 --> 00:28:29,720
+Sodomonas aeruginosa هي Non-Gelicose Fermenter لكن
+
+388
+00:28:29,720 --> 00:28:33,300
+ال Antirobacteriasy هي Gelicose Fermenter يبقى
+
+389
+00:28:33,300 --> 00:28:36,880
+هدول الفحصين بيفرقوا لبين ال Antirobacteriasy و
+
+390
+00:28:36,880 --> 00:28:37,820
+Sodomonas
+
+391
+00:28:40,250 --> 00:28:43,910
+كيف انا بدي اكشف عن هذا الانزيم in vitro قلنا
+
+392
+00:28:43,910 --> 00:28:47,410
+بنجيب substrate تشبه ال substrate اللي بيشتغل
+
+393
+00:28:47,410 --> 00:28:51,530
+عليها ال enzyme ال Oxidase test ممكن يكون فيه اندي
+
+394
+00:28:51,530 --> 00:28:55,630
+reagent ممكن يكون strip و ممكن يكون desk بقولي ال
+
+395
+00:28:55,630 --> 00:28:58,130
+reagent او ال substrate اللي هيشتغل عليها ال
+
+396
+00:28:58,130 --> 00:29:03,870
+enzyme ممكن يكون تترا مثال كينايل امين داي امين
+
+397
+00:29:03,870 --> 00:29:11,670
+داي هيدرو كلوراي اوأماينو داي مثل الألانين يبقى ها
+
+398
+00:29:11,670 --> 00:29:15,650
+هي ال substrate اللى هى يشتغل عليها ال enzyme عندى
+
+399
+00:29:15,650 --> 00:29:19,810
+انا بدي اكشف عن ال cytochrome c أكسيداز وقوللى أن
+
+400
+00:29:19,810 --> 00:29:24,110
+cytochrome c أكسيداز هيشتغل على substrate شبيه
+
+401
+00:29:24,110 --> 00:29:28,410
+بالcytochrome c هى هى ال substrate تترا مثال
+
+402
+00:29:28,410 --> 00:29:34,370
+فينايلDiamine هي الـ Substrate اللي هيشتغل عليها
+
+403
+00:29:34,370 --> 00:29:38,770
+الإنزايم شبيهة بـ Cytochrome C Reduced Cytochrome
+
+404
+00:29:38,770 --> 00:29:43,990
+C شبيهة فيها هيشتغل عليها أنزيم الـ Oxidase هاي
+
+405
+00:29:43,990 --> 00:29:47,550
+بتكون مكتسبة إلكترون بقول ليه بيكون لونها
+
+406
+00:29:47,550 --> 00:29:51,290
+Colorless لو موجود عند الإنزيم هيصير Oxidation
+
+407
+00:29:51,290 --> 00:29:57,490
+هيصير في عندي Oxidationهتفقد electron هيفقد هتفقد
+
+408
+00:29:57,490 --> 00:30:01,830
+electron و هتصير كانت هي hand reduced هتصير
+
+409
+00:30:01,830 --> 00:30:06,070
+oxidized بقوللي لما تكون oxidized بتحول لونها للون
+
+410
+00:30:06,070 --> 00:30:10,610
+purple يبقى لما تكون reduced بتكون لونها colorless
+
+411
+00:30:10,610 --> 00:30:15,390
+لكن لما تكون oxidized تكون لونها purple اذا ملاحظ
+
+412
+00:30:15,390 --> 00:30:19,060
+في الصورة كان في عندي hand رابطةصار في عندي رابطة
+
+413
+00:30:19,060 --> 00:30:22,900
+ثنائية يبقى أنا فقدت إلكترون هذا الإلكترون راح
+
+414
+00:30:22,900 --> 00:30:26,800
+للأكسجن للمستقبل النهائي للإلكترونات مع الـ H بلس
+
+415
+00:30:26,800 --> 00:30:31,200
+الموجود في الإلكترون الترانسبورت جان وكان والي الـ
+
+416
+00:30:31,200 --> 00:30:35,480
+water فبالتالي هك أنا بقدر أكشف على الـ cytochrome
+
+417
+00:30:35,480 --> 00:30:39,940
+C أكسيداز إذا أتحول اللون للون purple يبقى النتيجة
+
+418
+00:30:39,940 --> 00:30:43,660
+positive في عندي إنزيم الأكسيداز لأنهالـ
+
+419
+00:30:43,660 --> 00:30:50,360
+cytochrome-C يتقلل ولكن الـ Oxidized بيكون لونه
+
+420
+00:30:50,360 --> 00:30:55,740
+باربر كيف طريقة الشغل بقولي بتاخدي كولوني
+
+421
+00:30:55,740 --> 00:31:00,020
+للبكتيريا اللى بدك تكشف عندها انزيم ال Oxidized
+
+422
+00:31:00,020 --> 00:31:04,600
+ولا لأ او wooden toothpick بقولي في نفس اللى
+
+423
+00:31:04,600 --> 00:31:08,800
+بنعملهم او اللى هم نكشات لصناعة طبعا wooden
+
+424
+00:31:08,800 --> 00:31:13,170
+toothpick ليش بقولي لازم تستخدميوودون توث بيك أو
+
+425
+00:31:13,170 --> 00:31:19,170
+تستخدمي بلاستيك لوب ممنوع تستخدمي الـ Necrum Loop
+
+426
+00:31:19,170 --> 00:31:24,450
+لأن الـ Reagent ممكن يتفاعل مع الـ metal لو أنا
+
+427
+00:31:24,450 --> 00:31:28,150
+استخدمت Necrum Loop الـ Reagent يتفاعل مع الـ
+
+428
+00:31:28,150 --> 00:31:31,310
+metal فبالتالي بيعطيني false positive result عشان
+
+429
+00:31:31,310 --> 00:31:36,110
+هيك بستخدم اما بلاستيك لوب أو وودون توث بيك بقولي
+
+430
+00:31:36,110 --> 00:31:40,030
+بتاخدي هاي البكتيريا و بتحطيها على ال Oxidase Test
+
+431
+00:31:40,220 --> 00:31:44,100
+هذا الـ slide اسمه فيان دي أكسداز تستسلايد بتكون
+
+432
+00:31:44,100 --> 00:31:49,740
+مغمورة بالرياجينت بالأكسداز الرياجينت الموجود في
+
+433
+00:31:49,740 --> 00:31:54,640
+السبسترات اللي هو اللي قلناه قبل شوية الـ
+
+434
+00:31:54,640 --> 00:31:58,490
+tetramethyl phenylDiamine هذه هي الـ Substrate
+
+435
+00:31:58,490 --> 00:32:04,950
+بتكون مغمورة بالـ Oxidase test slide بتكون مغمورة
+
+436
+00:32:04,950 --> 00:32:08,870
+فيها بقولّي ال reaction بتتحول للون purple to
+
+437
+00:32:08,870 --> 00:32:11,550
+appear in the presence of cytochrome oxidase لو
+
+438
+00:32:11,550 --> 00:32:14,570
+عندي ال enzyme هيتحول اللون للون purple لأن ال
+
+439
+00:32:14,570 --> 00:32:17,710
+substrate لونها colorless لما تصير في حالة
+
+440
+00:32:17,710 --> 00:32:22,390
+oxidized لما تصير لها oxidation بتتحول لونها للون
+
+441
+00:32:22,390 --> 00:32:26,960
+purpleواللي بلاحظ تغير اللون بشكل يعني بشكل
+
+442
+00:32:26,960 --> 00:32:30,620
+انتقالي بيتحول اللون أول شيء من pink ل maroon
+
+443
+00:32:30,620 --> 00:32:34,900
+بعدين بتتحول للون purple بقرأ النتيجة خلال عشرين
+
+444
+00:32:34,900 --> 00:32:39,840
+ثانية لازم أني أنا أقرأ النتيجة خلال عشرين ثانية
+
+445
+00:32:39,840 --> 00:32:43,620
+لأن ممكن بعدهاك ال reagent يتأثر بالأكسجين الموجود
+
+446
+00:32:43,620 --> 00:32:46,840
+بالهواء ويعطيني false positive result و اللي في
+
+447
+00:32:46,840 --> 00:32:50,740
+عندي طريقة بديلة أني أنا بجيب ال oxidase reagent
+
+448
+00:32:50,740 --> 00:32:56,220
+ال reagent اللي هيمحضر بيكون و بديروه بشكل مباشر
+
+449
+00:32:56,220 --> 00:32:59,600
+على الكلون الموجودة في الأجار لو اتحول اللون للون
+
+450
+00:32:59,600 --> 00:33:03,640
+بيربول يبقى النتيجة بتكون positive ملاحظاتها ما
+
+451
+00:33:03,640 --> 00:33:06,880
+بقول لي pick your iniculum not with a metal loop و
+
+452
+00:33:06,880 --> 00:33:09,960
+لممنوع تستخدم ال metal loop لازم تستخدم ال plastic
+
+453
+00:33:09,960 --> 00:33:16,020
+loop أو الودن الودن الودن توف pick ليش لأن ال
+
+454
+00:33:16,020 --> 00:33:18,840
+reaction ممكن يتفاعل مع ال metal و يعطيني false
+
+455
+00:33:18,840 --> 00:33:26,020
+positive resultنبقى الحرارة مفرومة أو غير مفتوحة
+
+456
+00:33:26,020 --> 00:33:29,830
+في الحجم حتى تكون محتاجةold reagent is sitting out
+
+457
+00:33:29,830 --> 00:33:33,010
+on the bench and is bearable ask for a new tube
+
+458
+00:33:33,010 --> 00:33:37,350
+for the instructor بقوللي ال reagent اللي
+
+459
+00:33:37,350 --> 00:33:43,370
+بتستخدميه بتخليه بالتلاجة بتخليه مسكر ممنوع تخليه
+
+460
+00:33:43,370 --> 00:33:49,130
+مفتوح فقط عند الحاجة بتستخدميه لو كان old reagent
+
+461
+00:33:49,130 --> 00:33:53,810
+ممنوع انك تستخدميه لأنه ممكن يتغير مع العوامل
+
+462
+00:33:53,810 --> 00:33:58,170
+الخارجية ويعطيني false resultضروري انا اي فحص
+
+463
+00:33:58,170 --> 00:34:01,070
+كيميائي استخدم young culture او fresh culture
+
+464
+00:34:01,070 --> 00:34:04,110
+ممنوع انا استخدم all culture لأن الانزيم activity
+
+465
+00:34:04,110 --> 00:34:08,130
+بقل مع الوقت قلنا fresh reagent لازم انا استخدم ال
+
+466
+00:34:08,130 --> 00:34:12,130
+reagent يكون fresh اخده من ال freezer ممنوع يكون
+
+467
+00:34:12,130 --> 00:34:16,090
+open مفتوح في ال tube لأن قلنا ال reagent ممكن
+
+468
+00:34:16,090 --> 00:34:19,850
+يتغير مع العوامل الخارجية ويعطيني false positive
+
+469
+00:34:19,850 --> 00:34:24,490
+result بقرأ النتيجة خلال عشرين second not after
+
+470
+00:34:24,490 --> 00:34:29,130
+ليش لأنهممكن أن الأكسجين يتفاعل مع الـreagent
+
+471
+00:34:29,130 --> 00:34:32,130
+ويعطيني false positive result عشان هيك بقرأ
+
+472
+00:34:32,130 --> 00:34:38,330
+النتيجة خلال 20 ثانية موريكزيلا كاتريلز and
+
+473
+00:34:38,330 --> 00:34:41,010
+نيسيريا are a gram negative to double كُكّا
+
+474
+00:34:41,010 --> 00:34:43,430
+يتعرفنا على الـgram negative بس الله يقولنا اللي
+
+475
+00:34:43,430 --> 00:34:45,950
+هي الـsolomonas و الـenterobacteriasis كذا بفرق
+
+476
+00:34:45,950 --> 00:34:49,850
+بينهم و بفحص الأكسداز و فحص الجليكوز fermentation
+
+477
+00:34:50,520 --> 00:34:53,540
+بيندي لجرام نيجاتيف تيبلو كوكاى اللى هى ال موريكز
+
+478
+00:34:53,540 --> 00:34:57,380
+الكاتريليز و نيسيريا تنتهى ان اكسيداس positive و
+
+479
+00:34:57,380 --> 00:35:01,140
+كتالياز positive كيف افرق بينهم بيندي فحص ال
+
+480
+00:35:01,140 --> 00:35:05,060
+بتيوريات تست بتيوريات تست بيكشف ليه عن انزيم ال
+
+481
+00:35:05,060 --> 00:35:10,380
+بتيوريات استيرياز انزايم هذا الانزايمموجود في
+
+482
+00:35:10,380 --> 00:35:14,580
+الموريكزيلا كاتريلز و positive في الموريكزيلا
+
+483
+00:35:14,580 --> 00:35:17,560
+كاتريلز و negative في النيسيريا يبقى اللي بدنا
+
+484
+00:35:17,560 --> 00:35:20,900
+نعرفه ان انا عشان افرق الموريكزيلا عن النيسيريا في
+
+485
+00:35:20,900 --> 00:35:24,360
+عندى أنزيم او فحص ال biothiorate test بيكشف على
+
+486
+00:35:24,360 --> 00:35:27,700
+أنزيم ال biothiorate stress enzyme positive في
+
+487
+00:35:27,700 --> 00:35:32,210
+الموريكزيلا كاتريلز و negative في النيسيرياهذا ال
+
+488
+00:35:32,210 --> 00:35:35,530
+slide كملنا قلنا لو كان فيه اندي جرامستين ممكن
+
+489
+00:35:35,530 --> 00:35:38,070
+تكون جرام positive أو negative الجرام positive و
+
+490
+00:35:38,070 --> 00:35:42,270
+negative كل منها بنقسم لك كوكاي و بصلاي لو كانت
+
+491
+00:35:42,270 --> 00:35:45,790
+جرام positive بصلاي بعمل فحص ال catalase إذا طلعت
+
+492
+00:35:45,790 --> 00:35:47,850
+negative يبقى strepto إذا طلعت positive في
+
+493
+00:35:47,850 --> 00:35:51,770
+انديستافيل و كوكاي ماكرو كوكاي مايكرو كوكايكيف بدى
+
+494
+00:35:51,770 --> 00:35:56,230
+افرق بينهم بفحص الاكسداز فحص الاكسداز لو طلعت
+
+495
+00:35:56,230 --> 00:35:59,990
+اكسداز positive يبقى هي macro او micro لو طلعت
+
+496
+00:35:59,990 --> 00:36:03,390
+اكسداز negative يبقى هي Staphylococci طب كيف بدى
+
+497
+00:36:03,390 --> 00:36:06,670
+افرق Staphylococci species عن بعض بقول بفحص ال
+
+498
+00:36:06,670 --> 00:36:09,690
+coagulase لو coagulase positive يبقى هي
+
+499
+00:36:09,690 --> 00:36:12,910
+Staphylococcus aureus لو coagulase negative يبقى
+
+500
+00:36:12,910 --> 00:36:15,670
+هي Staphylococcus epidermidis او Staphylococcus
+
+501
+00:36:15,670 --> 00:36:20,070
+saprophyticبالنسبة لـ Ground Positive Bacilli بعمل
+
+502
+00:36:20,070 --> 00:36:22,710
+فحص الكيتالياز لو طلعت positive يبقى بيبقى باصلص
+
+503
+00:36:22,710 --> 00:36:26,570
+أو الستيريا مونو سايتوجين لو طلعت negative يبقى هي
+
+504
+00:36:26,570 --> 00:36:29,750
+Clostridium وكيف ده أفرق الباصلص عن الستيريا مونو
+
+505
+00:36:29,750 --> 00:36:32,630
+سايتوجين بالاسباريستان الباصلص sport farming
+
+506
+00:36:32,630 --> 00:36:37,550
+الاستيريا مونو سايتوجين non sport farmingالجرام
+
+507
+00:36:37,550 --> 00:36:41,550
+نيجاتيف ديبلوكوكاي كتلاز والأكسداز لإلهم positive
+
+508
+00:36:41,550 --> 00:36:44,230
+اللي هي الموريكزيلا والنيسيريا عشان أفرق بينهم
+
+509
+00:36:44,230 --> 00:36:47,490
+بعمل فحص الperturate استيراز ال positive
+
+510
+00:36:47,490 --> 00:36:50,770
+الموريكزيلا والنيجاتيف النيسيريا الجرام نيجاتيف
+
+511
+00:36:50,770 --> 00:36:56,810
+باسلايالجرام نيجاتيب بسال لايف بتضم الصدوموناس
+
+512
+00:36:56,810 --> 00:37:00,050
+الهيليكوباكترياسي وكمان في عندي الاسينيتوباكتر ال
+
+513
+00:37:00,050 --> 00:37:03,970
+Vibrio الهيليكوباكتر بقول عشان افرق بينهم بعمل فحص
+
+514
+00:37:03,970 --> 00:37:06,930
+الاكسيداز لو طلعت positive يبقى احتمال عندي لو
+
+515
+00:37:06,930 --> 00:37:11,270
+طلعت اكسيداز positive اما صدوموناس او Vibrio لو
+
+516
+00:37:11,270 --> 00:37:14,510
+طلعت اكسيداز نيجاتيب قلنا الهيليكوباكترياسي في
+
+517
+00:37:14,510 --> 00:37:18,070
+كمان الاسينيتوباكتر كيف بدي افرق الصدوموناس عن ال
+
+518
+00:37:18,070 --> 00:37:22,640
+Vibrio بقول بفحص الجليكوزلو طلعت negative يبقى هي
+
+519
+00:37:22,640 --> 00:37:25,560
+Sodomonas لو طلعت positive هي Vibrio أو
+
+520
+00:37:25,560 --> 00:37:29,580
+Helicobacter كيف أفرق ال Acetobacter و ال
+
+521
+00:37:29,580 --> 00:37:32,100
+Enterobacteriasi لو طلع ال Oxidase negative بقولي
+
+522
+00:37:32,100 --> 00:37:35,020
+برضه بفحص الجليكوز fermenter لو طلعت negative فهي
+
+523
+00:37:35,020 --> 00:37:38,000
+Acetobacter لو طلعت positive يبقى هي
+
+524
+00:37:38,000 --> 00:37:40,780
+Enterobacteriasi ال Enterobacteriasi قلنا عائلة
+
+525
+00:37:40,780 --> 00:37:43,620
+كبيرة ممكن انها تكون بتقسم لLactose fermenter و
+
+526
+00:37:43,620 --> 00:37:47,310
+Non-lactose fermenterلو طلعت Lactose Fermenter
+
+527
+00:37:47,310 --> 00:37:51,770
+بتنقسم لفيكل ونونفيكل الفيكل فيه عند الـ E.coli وE
+
+528
+00:37:51,770 --> 00:37:53,630
+.clepsula الـ non-fecal للـ Enterobacter
+
+529
+00:37:53,630 --> 00:37:57,510
+والستروبكتر كيف بفرق بينهم بفحص السيطرات E.coli
+
+530
+00:37:57,510 --> 00:38:00,250
+negative لكن الباقي positive أو فحص الـ motility
+
+531
+00:38:00,250 --> 00:38:03,450
+ال E.clepsula non-motile والباقي motile يبقى هذا
+
+532
+00:38:03,450 --> 00:38:08,790
+هو هيك تقريبا حاولنا أن نلخص الفحوصات عشان نتعرف
+
+533
+00:38:08,790 --> 00:38:14,590
+على البكتيرياهذه هي ال slide ال oxidase slide test
+
+534
+00:38:14,590 --> 00:38:17,930
+هاي ال slide بتكون مغمورة بال reagent بال
+
+535
+00:38:17,930 --> 00:38:22,290
+substrate لما أنا أخد البكتيريا أخدت البكتيريا و
+
+536
+00:38:22,290 --> 00:38:25,670
+حطيتها على هاي ال slide صار اللون أو ما تغير كان
+
+537
+00:38:25,670 --> 00:38:29,570
+colorless يبقى هي oxidase negative بكتيريا لكن لو
+
+538
+00:38:29,570 --> 00:38:32,970
+اتغير اللون للون purple يبقى هي oxidase positive
+
+539
+00:38:32,970 --> 00:38:38,340
+بكتيرياهنا في عندي filter paper انا باغمره
+
+540
+00:38:38,340 --> 00:38:42,860
+بالرياجانت وبعدين باخد البكتيريا اللى اتحول للون
+
+541
+00:38:42,860 --> 00:38:45,660
+بيربول يبقى اكسيداز positive لو ضاله لون colorless
+
+542
+00:38:45,660 --> 00:38:49,600
+يبقى اكسيداز negative هنا بشكل مباشر اجبت
+
+543
+00:38:49,600 --> 00:38:52,880
+الرياجانت وغمرته بالكولوني اذا اتحول لون الكولوني
+
+544
+00:38:52,880 --> 00:38:54,680
+للون بيربول يبقى positive
+
+545
+00:38:57,100 --> 00:39:02,100
+جبت slide حطيت عليها اللى هو ال filter بابر غمرت
+
+546
+00:39:02,100 --> 00:39:06,600
+ال reagent أنا غمرت ال reagent بيه ال filter حطيت
+
+547
+00:39:06,600 --> 00:39:09,740
+ال reagent على ال filter بابر بعدين جبت البكتيريا
+
+548
+00:39:10,150 --> 00:39:13,750
+وشفت إذا اتحول اللون للون violet يبقى هي positive
+
+549
+00:39:13,750 --> 00:39:18,510
+لكن لو ما اتغير يبقى هي negative يبقى عندي أكتر من
+
+550
+00:39:18,510 --> 00:39:21,890
+طريقة للفحص إما يكون ال reagent ال reagent بشكل
+
+551
+00:39:21,890 --> 00:39:24,990
+مباشر أديره على ال colony أو أجيب filter بابر أغمر
+
+552
+00:39:24,990 --> 00:39:28,670
+ال reagent أنا بعدين أحط البكتيريا ممكن يكون في
+
+553
+00:39:28,670 --> 00:39:33,340
+عندي اللي هو stripهي الـ Strip أو الـ Slide بيكون
+
+554
+00:39:33,340 --> 00:39:36,460
+مغمورة بالاصل بالـ reagent أنا فقط بضيف الـ Colony
+
+555
+00:39:36,460 --> 00:39:40,020
+ممكن يكون في عندي desk ال desk هذا برضه نفس الفكرة
+
+556
+00:39:40,020 --> 00:39:45,540
+بيكون مغمور بالـ reagent بمسك ال desk بنيدل وباخد
+
+557
+00:39:45,540 --> 00:39:49,400
+Colony من الطبق إذا اتحول اللون ال desk للون
+
+558
+00:39:49,400 --> 00:39:52,380
+violet يبقى هو positive يبقى باختلاف الطرق إذا
+
+559
+00:39:52,380 --> 00:39:55,240
+اتحول اللون للون violet أو dark purple يبقى هي
+
+560
+00:39:55,240 --> 00:39:59,860
+positive لكن لو كان colorless فهي negative هيك
+
+561
+00:39:59,860 --> 00:40:03,950
+بنكون خلصناالـ Citrate Utilization and Oxidase
+
+562
+00:40:03,950 --> 00:40:08,710
+Test الاتنين Indo Enzyme يعطيكم العافية جميعا
+
+563
+00:40:08,710 --> 00:40:11,350
+والسلام عليكم ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ca4bc2mptiY_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ca4bc2mptiY_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..b7d942fe8b76b48a28c9b038a39e5848c01c5867
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ca4bc2mptiY_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 2896, "start": 2.14, "end": 28.96, "text": "السلام عليكم ورحمة الله وبركاته في هذا اللقاء هتكون المحاضرة بعنوان Citrate Utilization and Oxidase Production هنتكلم عن إنزيم الـ Citrate وعن إنزيم الـ Oxidase وعرفنا إن هدول الإنزيمين يعتبروا Endoenzyme هنبدأ بـ Citrate Utilization استهلاك الـ Citrate ذكرنا سابقا أنواع الـ enzymatic reaction", "tokens": [6027, 3794, 37440, 25894, 24793, 4032, 2288, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224, 8978, 23758, 13672, 4587, 16606, 8032, 2655, 30544, 9673, 5016, 46958, 25720, 45030, 1863, 2407, 7649, 18435, 4404, 12555, 388, 2144, 293, 16489, 327, 651, 30088, 8032, 29399, 4117, 19528, 18871, 36145, 11622, 32640, 2423, 39184, 18435, 4404, 4032, 3615, 1863, 36145, 11622, 32640, 2423, 39184, 16489, 327, 651, 4032, 3615, 28480, 8315, 36145, 8032, 3215, 12610, 33688, 1863, 11622, 32640, 9957, 7251, 34268, 26890, 14407, 6967, 78, 268, 1229, 1398, 8032, 1863, 44510, 10721, 4724, 39184, 18435, 4404, 12555, 388, 2144, 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"word": " زائد", "probability": 0.5021158854166666}, {"start": 182.33, "end": 182.99, "word": " أسيتات", "probability": 0.7376708984375}], "temperature": 1.0}, {"id": 8, "seek": 20826, "start": 183.58, "end": 208.26, "text": "كمان البكتيريا اللى عندها القدرة أو اللى عندها انزيم استيتريات لياز في عندها انزيم الـ oxaloacetic acid decarboxylase هتنزع الـ CO2 الكربون من ال oxaloacetic acid هيتكون عندى بيروفات البيروفات هو ال carbon source هو ال carbon source اللى لازم لنمو البكتيريا وانقسمها", "tokens": [24793, 7649, 29739, 4117, 2655, 13546, 25528, 13672, 7578, 43242, 11296, 25062, 3215, 25720, 34051, 13672, 7578, 43242, 11296, 16472, 11622, 32640, 44713, 36081, 16572, 9307, 32239, 31377, 8978, 43242, 11296, 16472, 11622, 32640, 2423, 39184, 5976, 10334, 326, 3532, 8258, 979, 289, 4995, 5088, 651, 8032, 2655, 1863, 11622, 3615, 2423, 39184, 3002, 17, 33251, 25513, 11536, 9154, 2423, 5976, 10334, 326, 3532, 8258, 8032, 36081, 30544, 43242, 7578, 4724, 13546, 38688, 9307, 2423, 21292, 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"word": " انزيم", "probability": 0.7142740885416666}, {"start": 187.1, "end": 187.78, "word": " استيتريات", "probability": 0.5772705078125}, {"start": 187.78, "end": 188.24, "word": " لياز", "probability": 0.800048828125}, {"start": 188.24, "end": 188.38, "word": " في", "probability": 0.330078125}, {"start": 188.38, "end": 188.66, "word": " عندها", "probability": 0.888671875}, {"start": 188.66, "end": 189.02, "word": " انزيم", "probability": 0.9427083333333334}, {"start": 189.02, "end": 189.32, "word": " الـ", "probability": 0.630615234375}, {"start": 189.32, "end": 190.48, "word": " oxaloacetic", "probability": 0.861572265625}, {"start": 190.48, "end": 191.26, "word": " acid", "probability": 0.94140625}, {"start": 191.26, "end": 192.36, "word": " decarboxylase", "probability": 0.92177734375}, {"start": 192.36, "end": 193.28, "word": " هتنزع", "probability": 0.9439453125}, {"start": 193.28, "end": 194.32, "word": " الـ", "probability": 0.802490234375}, {"start": 194.32, "end": 195.38, "word": " CO2", "probability": 0.7763671875}, {"start": 195.38, "end": 196.1, "word": " الكربون", "probability": 0.7884928385416666}, {"start": 196.1, "end": 196.28, "word": " من", "probability": 0.9853515625}, {"start": 196.28, "end": 196.42, "word": " ال", "probability": 0.8681640625}, {"start": 196.42, "end": 197.36, "word": " oxaloacetic", "probability": 0.8145751953125}, {"start": 197.36, "end": 197.64, "word": " acid", "probability": 0.978515625}, {"start": 197.64, "end": 198.2, "word": " هيتكون", "probability": 0.76806640625}, {"start": 198.2, "end": 198.64, "word": " عندى", "probability": 0.8583984375}, {"start": 198.64, "end": 199.36, "word": " بيروفات", "probability": 0.7236328125}, {"start": 199.36, "end": 200.26, "word": " البيروفات", "probability": 0.738427734375}, {"start": 200.26, "end": 200.66, "word": " هو", "probability": 0.9541015625}, {"start": 200.66, "end": 201.0, "word": " ال", "probability": 0.9208984375}, {"start": 201.0, "end": 201.44, "word": " carbon", "probability": 0.65185546875}, {"start": 201.44, "end": 202.06, "word": " source", "probability": 0.884765625}, {"start": 202.06, "end": 203.0, "word": " هو", "probability": 0.9228515625}, {"start": 203.0, "end": 203.2, "word": " ال", "probability": 0.92919921875}, {"start": 203.2, "end": 203.56, "word": " carbon", "probability": 0.888671875}, {"start": 203.56, "end": 204.2, "word": " source", "probability": 0.9150390625}, {"start": 204.2, "end": 205.06, "word": " اللى", "probability": 0.8271484375}, {"start": 205.06, "end": 205.8, "word": " لازم", "probability": 0.9949544270833334}, {"start": 205.8, "end": 206.74, "word": " لنمو", "probability": 0.970703125}, {"start": 206.74, "end": 207.5, "word": " البكتيريا", "probability": 0.9416015625}, {"start": 207.5, "end": 208.26, "word": " وانقسمها", "probability": 0.96875}], "temperature": 1.0}, {"id": 9, "seek": 23445, "start": 208.81, "end": 234.45, "text": "أحنا قلنا إن الـ oxaloacetic acid هيعمل decarboxylase هينزع الـ CO2 في عندي الصوديوم الصوديوم الـ excess من الصوديوم مع الـ CO2 هيتكون عندي Na2CO3 هذا يعتبر alkaline فبالتالي هيرفعلي الـ pH يبقى صار عندي مصدر كربون من non carbohydrate carbon source من الـ citrate", "tokens": [10721, 5016, 8315, 12174, 1211, 8315, 36145, 2423, 39184, 5976, 10334, 326, 3532, 8258, 39896, 25957, 1211, 979, 289, 4995, 5088, 651, 39896, 1863, 11622, 3615, 2423, 39184, 3002, 17, 8978, 18871, 16254, 31767, 2407, 16254, 20498, 31767, 2407, 16254, 20498, 2423, 39184, 9310, 9154, 31767, 2407, 16254, 20498, 20449, 2423, 39184, 3002, 17, 39896, 2655, 30544, 18871, 16254, 6056, 17, 12322, 18, 23758, 7251, 34268, 26890, 44220, 533, 6156, 3555, 6027, 2655, 6027, 1829, 39896, 28480, 3615, 20292, 2423, 39184, 21677, 7251, 3555, 4587, 7578, 20328, 9640, 18871, 16254, 3714, 9381, 3215, 2288, 9122, 25513, 11536, 9154, 2107, 47048, 5954, 4009, 9154, 2423, 39184, 4814, 4404], "avg_logprob": -0.25072339011563194, "compression_ratio": 1.6265560165975104, "no_speech_prob": 1.1920928955078125e-07, "words": [{"start": 208.81, "end": 209.05, "word": "أحنا", "probability": 0.5977783203125}, {"start": 209.05, "end": 209.43, "word": " قلنا", "probability": 0.8958333333333334}, {"start": 209.43, "end": 209.69, "word": " إن", "probability": 0.47119140625}, {"start": 209.69, "end": 209.91, "word": " الـ", "probability": 0.51123046875}, {"start": 209.91, "end": 210.77, "word": " oxaloacetic", "probability": 0.76715087890625}, {"start": 210.77, "end": 211.07, "word": " acid", "probability": 0.8916015625}, {"start": 211.07, "end": 211.55, "word": " هيعمل", "probability": 0.8759765625}, {"start": 211.55, "end": 212.65, "word": " decarboxylase", "probability": 0.84609375}, {"start": 212.65, "end": 213.15, "word": " هينزع", "probability": 0.8514404296875}, {"start": 213.15, "end": 213.35, "word": " الـ", "probability": 0.849853515625}, {"start": 213.35, "end": 213.97, "word": " CO2", "probability": 0.733642578125}, {"start": 213.97, "end": 214.59, "word": " في", "probability": 0.6845703125}, {"start": 214.59, "end": 215.05, "word": " عندي", "probability": 0.737548828125}, {"start": 215.05, "end": 215.91, "word": " الصوديوم", "probability": 0.638671875}, {"start": 215.91, "end": 216.63, "word": " الصوديوم", "probability": 0.84698486328125}, {"start": 216.63, "end": 216.77, "word": " الـ", "probability": 0.5511474609375}, {"start": 216.77, "end": 217.05, "word": " excess", "probability": 0.962890625}, {"start": 217.05, "end": 217.31, "word": " من", "probability": 0.97998046875}, {"start": 217.31, "end": 217.89, "word": " الصوديوم", "probability": 0.964599609375}, {"start": 217.89, "end": 218.11, "word": " مع", "probability": 0.98095703125}, {"start": 218.11, "end": 218.33, "word": " الـ", "probability": 0.710205078125}, {"start": 218.33, "end": 218.69, "word": " CO2", "probability": 0.87841796875}, {"start": 218.69, "end": 219.21, "word": " هيتكون", "probability": 0.6982421875}, {"start": 219.21, "end": 219.59, "word": " عندي", "probability": 0.77734375}, {"start": 219.59, "end": 221.79, "word": " Na2CO3", "probability": 0.8306884765625}, {"start": 221.79, "end": 222.31, "word": " هذا", "probability": 0.7353515625}, {"start": 222.31, "end": 222.91, "word": " يعتبر", "probability": 0.8860677083333334}, {"start": 222.91, "end": 224.19, "word": " alkaline", "probability": 0.853759765625}, {"start": 224.19, "end": 225.05, "word": " فبالتالي", "probability": 0.9757486979166666}, {"start": 225.05, "end": 225.91, "word": " هيرفعلي", "probability": 0.8768310546875}, {"start": 225.91, "end": 226.25, "word": " الـ", "probability": 0.724609375}, {"start": 226.25, "end": 226.91, "word": " pH", "probability": 0.59814453125}, {"start": 226.91, "end": 228.55, "word": " يبقى", "probability": 0.96875}, {"start": 228.55, "end": 228.79, "word": " صار", "probability": 0.91064453125}, {"start": 228.79, "end": 229.09, "word": " عندي", "probability": 0.876708984375}, {"start": 229.09, "end": 229.61, "word": " مصدر", "probability": 0.997802734375}, {"start": 229.61, "end": 230.23, "word": " كربون", "probability": 0.9070638020833334}, {"start": 230.23, "end": 230.53, "word": " من", "probability": 0.98583984375}, {"start": 230.53, "end": 230.81, "word": " non", "probability": 0.378662109375}, {"start": 230.81, "end": 231.63, "word": " carbohydrate", "probability": 0.6787109375}, {"start": 231.63, "end": 232.31, "word": " carbon", "probability": 0.89599609375}, {"start": 232.31, "end": 232.97, "word": " source", "probability": 0.80126953125}, {"start": 232.97, "end": 233.65, "word": " من", "probability": 0.97998046875}, {"start": 233.65, "end": 234.01, "word": " الـ", "probability": 0.46044921875}, {"start": 234.01, "end": 234.45, "word": " citrate", "probability": 0.83544921875}], "temperature": 1.0}, {"id": 10, "seek": 26408, "start": 235.24, "end": 264.08, "text": "بقول لي البكتيريا اللى عندها القدرة على استهلاك الـ Sodium Citrate إلى جانب استهلاك هلاء الـ Citrate عندها القدرة على استهلاك الـ Ammonia الـ NH4 Plus عندها القدرة على إنتاجه وتحويله لـ Ammonia هذا يعتبر Nitrogen Source يبقى صار عندي مصدر للنيتروجين ومصدر للكربون هيك البكتيريا بتقدر تنمو وتنقسم وتتكعفر", "tokens": [3555, 39648, 32239, 29739, 4117, 2655, 13546, 25528, 13672, 7578, 43242, 11296, 25062, 3215, 25720, 15844, 44713, 3224, 15040, 4117, 2423, 39184, 42059, 2197, 18435, 4404, 30731, 10874, 7649, 3555, 44713, 3224, 15040, 4117, 8032, 1211, 16606, 2423, 39184, 18435, 4404, 43242, 11296, 25062, 3215, 25720, 15844, 44713, 3224, 15040, 4117, 2423, 39184, 2012, 3317, 654, 2423, 39184, 31118, 19, 7721, 43242, 11296, 25062, 3215, 25720, 15844, 11933, 29399, 26108, 3224, 34683, 5016, 2407, 26895, 3224, 5296, 39184, 2012, 3317, 654, 23758, 7251, 34268, 26890, 37942, 7747, 29629, 7251, 3555, 4587, 7578, 20328, 9640, 18871, 16254, 3714, 9381, 3215, 2288, 24976, 1863, 36081, 2288, 29245, 9957, 4032, 2304, 9381, 3215, 2288, 24976, 4117, 25513, 11536, 39896, 4117, 29739, 4117, 2655, 13546, 25528, 39894, 28543, 2288, 6055, 1863, 2304, 2407, 34683, 1863, 4587, 38251, 34683, 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Ammonia", "probability": 0.8474934895833334}, {"start": 246.76, "end": 247.06, "word": " الـ", "probability": 0.8037109375}, {"start": 247.06, "end": 248.04, "word": " NH4", "probability": 0.92822265625}, {"start": 248.04, "end": 248.74, "word": " Plus", "probability": 0.26611328125}, {"start": 248.74, "end": 249.02, "word": " عندها", "probability": 0.9638671875}, {"start": 249.02, "end": 249.5, "word": " القدرة", "probability": 0.984375}, {"start": 249.5, "end": 249.76, "word": " على", "probability": 0.9365234375}, {"start": 249.76, "end": 250.5, "word": " إنتاجه", "probability": 0.9259033203125}, {"start": 250.5, "end": 251.72, "word": " وتحويله", "probability": 0.95185546875}, {"start": 251.72, "end": 252.9, "word": " لـ", "probability": 0.447509765625}, {"start": 252.9, "end": 253.68, "word": " Ammonia", "probability": 0.9148763020833334}, {"start": 253.68, "end": 254.2, "word": " هذا", "probability": 0.748046875}, {"start": 254.2, "end": 254.74, "word": " يعتبر", "probability": 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الـ Causar citrate broth is another medium used to test for citrate utilization طريقة أخرى للكشف على ال citrate utilization Causar citrate broth قلنا is based on this formulation and contains Sodium citrate as a single source of carbon بتحتوي على Sodium citrate كمصدر وحيد للكربون Ammonium salt", "tokens": [6027, 9566, 16572, 28671, 16712, 7649, 10632, 2423, 39184, 7544, 301, 289, 4814, 4404, 18872, 307, 1071, 6399, 1143, 281, 1500, 337, 4814, 4404, 37074, 23032, 16572, 28671, 5551, 34740, 7578, 5296, 23275, 8592, 5172, 15844, 2423, 4814, 4404, 37074, 7544, 301, 289, 4814, 4404, 18872, 12174, 1211, 8315, 307, 2361, 322, 341, 37642, 293, 8306, 42059, 2197, 4814, 4404, 382, 257, 2167, 4009, 295, 5954, 39894, 33753, 45865, 15844, 42059, 2197, 4814, 4404, 9122, 2304, 9381, 3215, 2288, 4032, 5016, 25708, 5296, 23275, 25513, 11536, 2012, 3317, 2197, 5139], "avg_logprob": -0.16955700713199573, "compression_ratio": 1.609442060085837, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 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النهائي في ال electron transport chain the enzyme which reduce molecular oxygen to water to complete the last link in the chain of aerobic respiration", "tokens": [4117, 33911, 39894, 30544, 2423, 39184, 3185, 1353, 339, 11505, 12, 34, 22807, 4724, 39648, 2423, 39184, 3185, 1353, 339, 11505, 12, 34, 5551, 4117, 3794, 25708, 31377, 307, 264, 14709, 24521, 294, 264, 6084, 5495, 5021, 4724, 39648, 32239, 31439, 2423, 7649, 11622, 32640, 28239, 3224, 16373, 1829, 8978, 2423, 6084, 5495, 5021, 264, 24521, 597, 5407, 19046, 9169, 281, 1281, 281, 3566, 264, 1036, 2113, 294, 264, 5021, 295, 11207, 22234, 1597, 7611], "avg_logprob": -0.2504111806813039, "compression_ratio": 1.5251141552511416, "no_speech_prob": 0.0, "words": [{"start": 1417.48, "end": 1417.84, "word": "كيف", "probability": 0.905029296875}, {"start": 1417.84, "end": 1418.46, "word": " بتكون", "probability": 0.85546875}, {"start": 1418.46, "end": 1419.0, "word": " الـ", "probability": 0.690673828125}, {"start": 1419.0, "end": 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"end": 1480.85, "word": " ل", "probability": 0.7373046875}, {"start": 1480.85, "end": 1481.49, "word": " الـ", "probability": 0.426513671875}, {"start": 1481.49, "end": 1481.89, "word": " reduced", "probability": 0.80810546875}, {"start": 1481.89, "end": 1482.55, "word": " cytochrome", "probability": 0.9810791015625}, {"start": 1482.55, "end": 1483.47, "word": "-C", "probability": 0.993408203125}, {"start": 1483.47, "end": 1483.87, "word": " كانت", "probability": 0.962646484375}, {"start": 1483.87, "end": 1484.69, "word": " مكتسبة", "probability": 0.885546875}, {"start": 1484.69, "end": 1485.51, "word": " إلكترونات", "probability": 0.9581705729166666}, {"start": 1485.51, "end": 1485.99, "word": " هتصير", "probability": 0.859130859375}, {"start": 1485.99, "end": 1486.67, "word": " فاقدة", "probability": 0.9200439453125}, {"start": 1486.67, "end": 1487.73, "word": " للإلكترونات", "probability": 0.9025530133928571}, {"start": 1487.73, "end": 1488.35, "word": " هتتحول", "probability": 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بتكون لونها colorless لكن لما تكون oxidized تكون لونها purple اذا ملاحظ في الصورة كان في عندي hand رابطة", "tokens": [3224, 2655, 5172, 28543, 6084, 8032, 33911, 28543, 8032, 2655, 5172, 28543, 6084, 4032, 8032, 2655, 9381, 13546, 25961, 2655, 39896, 1011, 9212, 8032, 2655, 9381, 13546, 19924, 1602, 4724, 39648, 20292, 5296, 15042, 6055, 30544, 19924, 1602, 39894, 5016, 12610, 5296, 11536, 11296, 5296, 1211, 11536, 9656, 7251, 3555, 4587, 7578, 5296, 15042, 6055, 30544, 9212, 39894, 30544, 5296, 11536, 11296, 2017, 1832, 44381, 5296, 15042, 6055, 30544, 19924, 1602, 6055, 30544, 5296, 11536, 11296, 9656, 1975, 15730, 3714, 15040, 5016, 19913, 8978, 31767, 13063, 3660, 25961, 8978, 18871, 16254, 1011, 12602, 16758, 9566, 3660], "avg_logprob": -0.14271907708079545, "compression_ratio": 1.9692307692307693, "no_speech_prob": 1.1920928955078125e-07, "words": [{"start": 1794.57, "end": 1795.41, "word": "هتفقد", "probability": 0.93896484375}, {"start": 1795.41, "end": 1796.27, "word": " 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الـ Substrate بتكون مغمورة بالـ Oxidase test slide بتكون مغمورة فيها بقولّي ال reaction بتتحول للون purple to appear in the presence of cytochrome oxidase لو عندي ال enzyme هيتحول اللون للون purple لأن ال substrate لونها colorless لما تصير في حالة oxidized لما تصير لها oxidation بتتحول لونها للون purple", "tokens": [35, 2918, 533, 29538, 39896, 2423, 39184, 42090, 4404, 39894, 30544, 3714, 17082, 2304, 13063, 3660, 20666, 39184, 16489, 327, 651, 1500, 4137, 39894, 30544, 3714, 17082, 2304, 13063, 3660, 8978, 11296, 4724, 39648, 11703, 1829, 2423, 5480, 39894, 2655, 5016, 12610, 5296, 1211, 11536, 9656, 281, 4204, 294, 264, 6814, 295, 3185, 1353, 339, 11505, 19924, 651, 45164, 43242, 1829, 2423, 24521, 39896, 2655, 5016, 12610, 13672, 11536, 5296, 1211, 11536, 9656, 5296, 33456, 2423, 27585, 5296, 11536, 11296, 2017, 1832, 5296, 15042, 6055, 9381, 13546, 8978, 11331, 6027, 3660, 19924, 1602, 5296, 15042, 6055, 9381, 13546, 5296, 11296, 36767, 39894, 2655, 5016, 12610, 5296, 11536, 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"probability": 0.741455078125}, {"start": 1938.43, "end": 1938.69, "word": " لما", "probability": 0.962158203125}, {"start": 1938.69, "end": 1939.33, "word": " تصير", "probability": 0.77685546875}, {"start": 1939.33, "end": 1939.75, "word": " لها", "probability": 0.754150390625}, {"start": 1939.75, "end": 1940.67, "word": " oxidation", "probability": 0.86669921875}, {"start": 1940.67, "end": 1941.59, "word": " بتتحول", "probability": 0.920654296875}, {"start": 1941.59, "end": 1941.97, "word": " لونها", "probability": 0.9912109375}, {"start": 1941.97, "end": 1942.39, "word": " للون", "probability": 0.9573567708333334}, {"start": 1942.39, "end": 1942.73, "word": " purple", "probability": 0.9833984375}], "temperature": 1.0}, {"id": 79, "seek": 197200, "start": 1943.12, "end": 1972.0, "text": "واللي بلاحظ تغير اللون بشكل يعني بشكل انتقالي بيتحول اللون أول شيء من pink ل maroon بعدين بتتحول للون purple بقرأ النتيجة خلال عشرين ثانية لازم أني أنا أقرأ النتيجة خلال عشرين ثانية لأن ممكن بعدهاك ال reagent يتأثر بالأكسجين الموجود بالهواء ويعطيني false positive result و اللي في عندي طريقة بديلة أني أنا بجيب ال oxidase reagent ال reagent اللي هي", "tokens": [2407, 6027, 20292, 4724, 15040, 5016, 19913, 6055, 17082, 13546, 13672, 11536, 4724, 8592, 28820, 37495, 22653, 4724, 8592, 28820, 16472, 2655, 4587, 6027, 1829, 4724, 36081, 5016, 12610, 13672, 11536, 5551, 12610, 44049, 38207, 9154, 7022, 5296, 1849, 4106, 39182, 9957, 39894, 2655, 5016, 12610, 5296, 1211, 11536, 9656, 4724, 4587, 2288, 10721, 28239, 31371, 7435, 3660, 16490, 1211, 6027, 6225, 46309, 9957, 38637, 7649, 10632, 5296, 31377, 2304, 14739, 1829, 41850, 5551, 4587, 2288, 10721, 28239, 31371, 7435, 3660, 16490, 1211, 6027, 6225, 46309, 9957, 38637, 7649, 10632, 5296, 33456, 3714, 43020, 39182, 11296, 4117, 2423, 26949, 317, 7251, 2655, 10721, 49115, 20666, 10721, 4117, 3794, 7435, 9957, 9673, 29245, 23328, 20666, 3224, 2407, 16606, 4032, 40228, 9566, 9957, 1829, 7908, 3353, 1874, 4032, 13672, 1829, 8978, 18871, 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1947.88, "end": 1948.3, "word": " بيتحول", "probability": 0.859130859375}, {"start": 1948.3, "end": 1948.56, "word": " اللون", "probability": 0.8759765625}, {"start": 1948.56, "end": 1948.78, "word": " أول", "probability": 0.78466796875}, {"start": 1948.78, "end": 1949.22, "word": " شيء", "probability": 0.605224609375}, {"start": 1949.22, "end": 1949.54, "word": " من", "probability": 0.876953125}, {"start": 1949.54, "end": 1950.02, "word": " pink", "probability": 0.859375}, {"start": 1950.02, "end": 1950.2, "word": " ل", "probability": 0.82177734375}, {"start": 1950.2, "end": 1950.62, "word": " maroon", "probability": 0.625732421875}, {"start": 1950.62, "end": 1951.0, "word": " بعدين", "probability": 0.6142578125}, {"start": 1951.0, "end": 1951.46, "word": " بتتحول", "probability": 0.7823486328125}, {"start": 1951.46, "end": 1951.9, "word": " للون", "probability": 0.9459635416666666}, {"start": 1951.9, "end": 1952.34, "word": " purple", "probability": 0.66552734375}, {"start": 1952.34, "end": 1953.22, "word": " بقرأ", "probability": 0.91015625}, {"start": 1953.22, "end": 1953.74, "word": " النتيجة", "probability": 0.962646484375}, {"start": 1953.74, "end": 1954.22, "word": " خلال", "probability": 0.9964192708333334}, {"start": 1954.22, "end": 1954.9, "word": " عشرين", "probability": 0.8040364583333334}, {"start": 1954.9, "end": 1955.54, "word": " ثانية", "probability": 0.8507486979166666}, {"start": 1955.54, "end": 1956.5, "word": " لازم", "probability": 0.970703125}, {"start": 1956.5, "end": 1956.72, "word": " أني", "probability": 0.667236328125}, {"start": 1956.72, "end": 1956.88, "word": " أنا", "probability": 0.56591796875}, {"start": 1956.88, "end": 1957.4, "word": " أقرأ", "probability": 0.9434814453125}, {"start": 1957.4, "end": 1957.92, "word": " النتيجة", "probability": 0.9661865234375}, {"start": 1957.92, "end": 1958.72, "word": " خلال", "probability": 0.9778645833333334}, {"start": 1958.72, "end": 1959.28, "word": " عشرين", "probability": 0.9459635416666666}, {"start": 1959.28, "end": 1959.84, "word": " ثانية", "probability": 0.99658203125}, {"start": 1959.84, "end": 1960.08, "word": " لأن", "probability": 0.8701171875}, {"start": 1960.08, "end": 1960.52, "word": " ممكن", "probability": 0.873046875}, {"start": 1960.52, "end": 1961.18, "word": " بعدهاك", "probability": 0.6868489583333334}, {"start": 1961.18, "end": 1961.26, "word": " ال", "probability": 0.9736328125}, {"start": 1961.26, "end": 1961.68, "word": " reagent", "probability": 0.62841796875}, {"start": 1961.68, "end": 1962.42, "word": " يتأثر", "probability": 0.9669189453125}, {"start": 1962.42, "end": 1963.14, "word": " بالأكسجين", "probability": 0.8446451822916666}, {"start": 1963.14, "end": 1963.62, "word": " الموجود", "probability": 0.9451497395833334}, {"start": 1963.62, "end": 1964.04, "word": " بالهواء", "probability": 0.8824462890625}, {"start": 1964.04, "end": 1964.66, "word": " ويعطيني", "probability": 0.85732421875}, {"start": 1964.66, "end": 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{"start": 2056.87, "end": 2057.41, "word": " العوامل", "probability": 0.9571533203125}, {"start": 2057.41, "end": 2057.97, "word": " الخارجية", "probability": 0.996337890625}, {"start": 2057.97, "end": 2058.57, "word": " ويعطيني", "probability": 0.79326171875}, {"start": 2058.57, "end": 2059.09, "word": " false", "probability": 0.66796875}, {"start": 2059.09, "end": 2059.85, "word": " positive", "probability": 0.91650390625}, {"start": 2059.85, "end": 2060.59, "word": " result", "probability": 0.962890625}, {"start": 2060.59, "end": 2061.51, "word": " بقرأ", "probability": 0.94482421875}, {"start": 2061.51, "end": 2061.95, "word": " النتيجة", "probability": 0.96875}, {"start": 2061.95, "end": 2062.33, "word": " خلال", "probability": 0.9970703125}, {"start": 2062.33, "end": 2062.79, "word": " عشرين", "probability": 0.729736328125}, {"start": 2062.79, "end": 2063.17, "word": " second", "probability": 0.71337890625}, {"start": 2063.17, "end": 2063.77, "word": " not", "probability": 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كُكّا", "probability": 0.4881103515625}, {"start": 2081.01, "end": 2081.47, "word": " يتعرفنا", "probability": 0.804248046875}, {"start": 2081.47, "end": 2081.59, "word": " على", "probability": 0.82177734375}, {"start": 2081.59, "end": 2082.05, "word": " الـgram", "probability": 0.6248372395833334}, {"start": 2082.05, "end": 2082.49, "word": " negative", "probability": 0.9208984375}, {"start": 2082.49, "end": 2082.75, "word": " بس", "probability": 0.920166015625}, {"start": 2082.75, "end": 2082.95, "word": " الله", "probability": 0.7841796875}, {"start": 2082.95, "end": 2083.31, "word": " يقولنا", "probability": 0.9742838541666666}, {"start": 2083.31, "end": 2083.43, "word": " اللي", "probability": 0.892822265625}, {"start": 2083.43, "end": 2083.55, "word": " هي", "probability": 0.791015625}, {"start": 2083.55, "end": 2084.23, "word": " الـsolomonas", "probability": 0.432958984375}, {"start": 2084.23, "end": 2084.29, "word": " و", "probability": 0.72900390625}, {"start": 2084.29, 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"text": "بيندي لجرام نيجاتيف تيبلو كوكاى اللى هى ال موريكز الكاتريليز و نيسيريا تنتهى ان اكسيداس positive و كتالياز positive كيف افرق بينهم بيندي فحص ال بتيوريات تست بتيوريات تست بيكشف ليه عن انزيم ال بتيوريات استيرياز انزايم هذا الانزايم", "tokens": [3555, 9957, 16254, 5296, 7435, 2288, 10943, 8717, 1829, 7435, 9307, 33911, 6055, 1829, 36150, 2407, 9122, 2407, 4117, 995, 7578, 13672, 7578, 8032, 7578, 2423, 3714, 13063, 1829, 4117, 11622, 33251, 9307, 16572, 20292, 11622, 4032, 8717, 1829, 3794, 13546, 25528, 6055, 29399, 3224, 7578, 16472, 1975, 4117, 3794, 25708, 32277, 3353, 4032, 9122, 2655, 6027, 1829, 31377, 3353, 9122, 33911, 1975, 5172, 2288, 4587, 49374, 16095, 4724, 9957, 16254, 6156, 5016, 9381, 2423, 39894, 1829, 13063, 1829, 9307, 6055, 14851, 39894, 1829, 13063, 1829, 9307, 6055, 14851, 4724, 1829, 4117, 8592, 5172, 32239, 3224, 18871, 16472, 11622, 32640, 2423, 39894, 1829, 13063, 1829, 9307, 44713, 13546, 1829, 31377, 16472, 11622, 995, 32640, 23758, 2423, 7649, 11622, 995, 32640], "avg_logprob": -0.42484503147030667, "compression_ratio": 1.9901477832512315, "no_speech_prob": 0.0, "words": [{"start": 2090.52, "end": 2090.94, "word": "بيندي", "probability": 0.50439453125}, {"start": 2090.94, "end": 2091.42, "word": " لجرام", "probability": 0.72271728515625}, {"start": 2091.42, "end": 2091.92, "word": " نيجاتيف", "probability": 0.6720703125}, {"start": 2091.92, "end": 2092.3, "word": " تيبلو", "probability": 0.67242431640625}, {"start": 2092.3, "end": 2092.72, "word": " كوكاى", "probability": 0.647412109375}, {"start": 2092.72, "end": 2092.82, "word": " اللى", "probability": 0.62353515625}, {"start": 2092.82, "end": 2093.0, "word": " هى", "probability": 0.756103515625}, {"start": 2093.0, "end": 2093.1, "word": " ال", "probability": 0.396484375}, {"start": 2093.1, "end": 2093.54, "word": " موريكز", "probability": 0.68359375}, {"start": 2093.54, "end": 2094.34, "word": " الكاتريليز", "probability": 0.4612060546875}, {"start": 2094.34, "end": 2094.62, "word": " و", "probability": 0.368408203125}, {"start": 2094.62, "end": 2095.18, "word": " نيسيريا", "probability": 0.71064453125}, {"start": 2095.18, "end": 2095.72, "word": " تنتهى", "probability": 0.6085205078125}, {"start": 2095.72, "end": 2095.8, "word": " ان", "probability": 0.250732421875}, {"start": 2095.8, "end": 2096.4, "word": " اكسيداس", "probability": 0.661376953125}, {"start": 2096.4, "end": 2096.86, "word": " positive", "probability": 0.59375}, {"start": 2096.86, "end": 2097.38, "word": " و", "probability": 0.6318359375}, {"start": 2097.38, "end": 2097.94, "word": " كتالياز", "probability": 0.61298828125}, {"start": 2097.94, "end": 2098.36, "word": " positive", "probability": 0.83837890625}, {"start": 2098.36, "end": 2098.72, "word": " كيف", "probability": 0.889892578125}, {"start": 2098.72, "end": 2099.32, "word": " افرق", "probability": 0.756103515625}, {"start": 2099.32, "end": 2100.08, "word": " بينهم", "probability": 0.80419921875}, {"start": 2100.08, "end": 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reagent أنا غمرت ال reagent بيه ال filter حطيت ال reagent على ال filter بابر بعدين جبت البكتيريا", "tokens": [7435, 3555, 2655, 4137, 11331, 9566, 36081, 25894, 11296, 13672, 7578, 31439, 2423, 6608, 4724, 16758, 2288, 32771, 29973, 2655, 2423, 26949, 317, 41850, 32771, 29973, 2655, 2423, 26949, 317, 4724, 1829, 3224, 2423, 6608, 11331, 9566, 36081, 2423, 26949, 317, 15844, 2423, 6608, 4724, 16758, 2288, 39182, 9957, 10874, 3555, 2655, 29739, 4117, 2655, 13546, 25528], "avg_logprob": -0.2355872790875106, "compression_ratio": 1.6842105263157894, "no_speech_prob": 7.152557373046875e-07, "words": [{"start": 2337.1, "end": 2337.76, "word": "جبت", "probability": 0.671875}, {"start": 2337.76, "end": 2338.18, "word": " slide", "probability": 0.455810546875}, {"start": 2338.18, "end": 2338.7, "word": " حطيت", "probability": 0.7389322916666666}, {"start": 2338.7, "end": 2339.4, "word": " عليها", "probability": 0.976318359375}, {"start": 2339.4, "end": 2339.84, "word": " اللى", "probability": 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purple يبقى هي positive لكن لو كان colorless فهي negative هيك بنكون خلصنا", "tokens": [3224, 1829, 2423, 39184, 745, 8400, 34051, 2423, 39184, 26405, 4724, 1829, 30544, 3714, 17082, 2304, 13063, 3660, 20666, 33546, 1211, 20666, 39184, 26949, 317, 41850, 6156, 47432, 4724, 11242, 33911, 2423, 39184, 4004, 2526, 3714, 43020, 7251, 30544, 8978, 18871, 16254, 10026, 2423, 10026, 23758, 4724, 43042, 3224, 8717, 36178, 27188, 4117, 25720, 4724, 1829, 30544, 3714, 17082, 2304, 13063, 20666, 39184, 26949, 317, 4724, 2304, 3794, 4117, 2423, 10026, 44945, 25708, 1211, 46599, 47283, 3215, 4004, 2526, 9154, 41950, 3555, 4587, 11933, 15730, 1975, 2655, 5016, 12610, 13672, 11536, 2423, 10026, 5296, 1211, 11536, 46480, 7251, 3555, 4587, 7578, 31439, 3353, 7251, 3555, 4587, 7578, 4724, 47283, 2655, 15040, 5172, 41950, 2288, 4587, 11933, 15730, 1975, 2655, 5016, 12610, 13672, 11536, 5296, 1211, 11536, 46480, 34051, 2877, 9656, 7251, 3555, 4587, 7578, 39896, 3353, 44381, 45164, 25961, 2017, 1832, 6156, 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"probability": 0.76171875}, {"start": 2378.38, "end": 2378.58, "word": " desk", "probability": 0.87353515625}, {"start": 2378.58, "end": 2378.88, "word": " هذا", "probability": 0.84912109375}, {"start": 2378.88, "end": 2379.26, "word": " برضه", "probability": 0.8966471354166666}, {"start": 2379.26, "end": 2379.6, "word": " نفس", "probability": 0.97802734375}, {"start": 2379.6, "end": 2380.02, "word": " الفكرة", "probability": 0.9908854166666666}, {"start": 2380.02, "end": 2380.32, "word": " بيكون", "probability": 0.90576171875}, {"start": 2380.32, "end": 2380.7, "word": " مغمور", "probability": 0.989013671875}, {"start": 2380.7, "end": 2381.0, "word": " بالـ", "probability": 0.60791015625}, {"start": 2381.0, "end": 2381.78, "word": " reagent", "probability": 0.954345703125}, {"start": 2381.78, "end": 2382.32, "word": " بمسك", "probability": 0.966796875}, {"start": 2382.32, "end": 2382.68, "word": " ال", "probability": 0.99072265625}, {"start": 2382.68, "end": 2383.14, "word": " desk", "probability": 0.9736328125}, {"start": 2383.14, "end": 2384.7, "word": " بنيدل", "probability": 0.7244466145833334}, {"start": 2384.7, "end": 2385.54, "word": " وباخد", "probability": 0.8291015625}, {"start": 2385.54, "end": 2386.54, "word": " Colony", "probability": 0.898193359375}, {"start": 2386.54, "end": 2386.82, "word": " من", "probability": 0.9921875}, {"start": 2386.82, "end": 2387.28, "word": " الطبق", "probability": 0.9816080729166666}, {"start": 2387.28, "end": 2387.44, "word": " إذا", "probability": 0.907958984375}, {"start": 2387.44, "end": 2387.86, "word": " اتحول", "probability": 0.87890625}, {"start": 2387.86, "end": 2388.3, "word": " اللون", "probability": 0.898193359375}, {"start": 2388.3, "end": 2388.76, "word": " ال", "probability": 0.96533203125}, {"start": 2388.76, "end": 2389.04, "word": " desk", "probability": 0.9189453125}, {"start": 2389.04, "end": 2389.4, "word": " للون", "probability": 0.8984375}, {"start": 2389.4, "end": 2389.78, "word": " violet", "probability": 0.62548828125}, {"start": 2389.78, "end": 2390.12, "word": " يبقى", "probability": 0.983154296875}, {"start": 2390.12, "end": 2390.38, "word": " هو", "probability": 0.97412109375}, {"start": 2390.38, "end": 2390.76, "word": " positive", "probability": 0.7275390625}, {"start": 2390.76, "end": 2391.06, "word": " يبقى", "probability": 0.887451171875}, {"start": 2391.06, "end": 2391.7, "word": " باختلاف", "probability": 0.96748046875}, {"start": 2391.7, "end": 2392.18, "word": " الطرق", "probability": 0.9700520833333334}, {"start": 2392.18, "end": 2392.38, "word": " إذا", "probability": 0.984619140625}, {"start": 2392.38, "end": 2392.84, "word": " اتحول", "probability": 0.9361572265625}, {"start": 2392.84, "end": 2393.14, "word": " اللون", "probability": 0.996337890625}, {"start": 2393.14, "end": 2393.5, "word": " للون", "probability": 0.9332682291666666}, {"start": 2393.5, "end": 2393.9, "word": " violet", "probability": 0.92822265625}, {"start": 2393.9, "end": 2394.12, "word": " أو", "probability": 0.89404296875}, {"start": 2394.12, "end": 2394.4, "word": " dark", "probability": 0.82568359375}, {"start": 2394.4, "end": 2394.78, "word": " purple", "probability": 0.9521484375}, {"start": 2394.78, "end": 2395.08, "word": " يبقى", "probability": 0.981689453125}, {"start": 2395.08, "end": 2395.24, "word": " هي", "probability": 0.80517578125}, {"start": 2395.24, "end": 2395.64, "word": " positive", "probability": 0.88916015625}, {"start": 2395.64, "end": 2396.26, "word": " لكن", "probability": 0.97265625}, {"start": 2396.26, "end": 2396.46, "word": " لو", "probability": 0.9853515625}, {"start": 2396.46, "end": 2396.74, "word": " كان", "probability": 0.99560546875}, {"start": 2396.74, "end": 2397.46, "word": " colorless", "probability": 0.761962890625}, {"start": 2397.46, "end": 2398.1, "word": " فهي", "probability": 0.8912760416666666}, {"start": 2398.1, "end": 2398.94, "word": " negative", "probability": 0.87353515625}, {"start": 2398.94, "end": 2399.86, "word": " هيك", "probability": 0.708740234375}, {"start": 2399.86, "end": 2400.16, "word": " بنكون", "probability": 0.806884765625}, {"start": 2400.16, "end": 2400.8, "word": " خلصنا", "probability": 0.9813232421875}], "temperature": 1.0}, {"id": 99, "seek": 241135, "start": 2401.21, "end": 2411.35, "text": "الـ Citrate Utilization and Oxidase Test الاتنين Indo Enzyme يعطيكم العافية جميعا والسلام عليكم ورحمة الله وبركاته", "tokens": [6027, 39184, 18435, 4404, 12555, 388, 2144, 293, 16489, 327, 651, 9279, 2423, 9307, 1863, 9957, 2333, 78, 2193, 1229, 1398, 37495, 9566, 1829, 24793, 18863, 31845, 10632, 10874, 2304, 40228, 995, 16070, 3794, 37440, 25894, 24793, 4032, 2288, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224], "avg_logprob": -0.20882161613553762, "compression_ratio": 1.1724137931034482, "no_speech_prob": 1.1265277862548828e-05, "words": [{"start": 2401.21, "end": 2401.71, "word": "الـ", "probability": 0.661376953125}, {"start": 2401.71, "end": 2402.15, "word": " Citrate", "probability": 0.801025390625}, {"start": 2402.15, "end": 2402.93, "word": " Utilization", "probability": 0.859375}, {"start": 2402.93, "end": 2403.37, "word": " and", "probability": 0.88525390625}, {"start": 2403.37, "end": 2403.95, "word": " Oxidase", "probability": 0.8982747395833334}, {"start": 2403.95, "end": 2404.29, "word": " Test", "probability": 0.7822265625}, {"start": 2404.29, "end": 2404.91, "word": " الاتنين", "probability": 0.672454833984375}, {"start": 2404.91, "end": 2405.69, "word": " Indo", "probability": 0.5181884765625}, {"start": 2405.69, "end": 2407.09, "word": " Enzyme", "probability": 0.7486165364583334}, {"start": 2407.09, "end": 2407.73, "word": " يعطيكم", "probability": 0.8741455078125}, {"start": 2407.73, "end": 2408.15, "word": " العافية", "probability": 0.8421223958333334}, {"start": 2408.15, "end": 2408.71, "word": " جميعا", "probability": 0.8834228515625}, {"start": 2408.71, "end": 2409.17, "word": " والسلام", "probability": 0.90185546875}, {"start": 2409.17, "end": 2409.63, "word": " عليكم", "probability": 0.947509765625}, {"start": 2409.63, "end": 2410.07, "word": " ورحمة", "probability": 0.9813232421875}, {"start": 2410.07, "end": 2410.43, "word": " الله", "probability": 0.96533203125}, {"start": 2410.43, "end": 2411.35, "word": " وبركاته", "probability": 0.9796875}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 2412.3443125, "duration_after_vad": 2366.797499999989}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ca4bc2mptiY_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ca4bc2mptiY_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..50129ed9497c4320274483909f4db8d38c7b46ec
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ca4bc2mptiY_raw.srt
@@ -0,0 +1,2252 @@
+1
+00:00:02,140 --> 00:00:06,040
+السلام عليكم ورحمة الله وبركاته في هذا اللقاء
+
+2
+00:00:06,040 --> 00:00:10,080
+هتكون المحاضرة بعنوان Citrate Utilization and
+
+3
+00:00:10,080 --> 00:00:14,940
+Oxidase Production هنتكلم عن إنزيم الـ Citrate وعن
+
+4
+00:00:14,940 --> 00:00:20,140
+إنزيم الـ Oxidase وعرفنا إن هدول الإنزيمين يعتبروا
+
+5
+00:00:20,140 --> 00:00:24,920
+Endoenzyme هنبدأ بـ Citrate Utilization استهلاك
+
+6
+00:00:24,920 --> 00:00:28,380
+الـ Citrate ذكرنا سابقا أنواع الـ enzymatic
+
+7
+00:00:28,380 --> 00:00:32,390
+reactionكان من ضمنها utilization of non
+
+8
+00:00:32,390 --> 00:00:36,630
+-carbohydrate carbon source ذكرنا مثال اليوريا
+
+9
+00:00:36,630 --> 00:00:41,530
+والسيطران طيب ليش البكتيريا بدها carbon source؟
+
+10
+00:00:41,530 --> 00:00:45,570
+بقول لي البكتيريا عشان تنمو وتتكثر بحاجة ل DNA
+
+11
+00:00:45,570 --> 00:00:49,450
+replication عشان يصير بعدها transcription بعدين
+
+12
+00:00:49,450 --> 00:00:54,030
+يصير ال translation لتكوين ال protein عشان يتكون ل
+
+13
+00:00:54,030 --> 00:00:58,280
+protein فهي بحاجة ل carbonand nitrogen source
+
+14
+00:00:58,280 --> 00:01:03,660
+البكتيريا راح تستهلك مصادر للحصول عليهم بالنسبة لل
+
+15
+00:01:03,660 --> 00:01:07,540
+carbon source ممكن انها تستهلك الكربوهيدرات الشكر
+
+16
+00:01:07,540 --> 00:01:12,280
+وممكن انها تستهلك من مصادر non carbohydrate زي
+
+17
+00:01:12,280 --> 00:01:16,960
+اليوريا ال amino acid السيترات اليوم موضوعنا هيكون
+
+18
+00:01:16,960 --> 00:01:22,140
+عن استهلاك السيترات كيف البكتيريا بتستهلك السيترات
+
+19
+00:01:22,140 --> 00:01:26,550
+للحصول على ال carbon sourceبالنسبة لأ ال nitrogen
+
+20
+00:01:26,550 --> 00:01:29,590
+source في عندي ال amino acid الأمانية هي تعتبر
+
+21
+00:01:29,590 --> 00:01:34,770
+nitrogen source لازمة لنمو البكتيريا السيتريات
+
+22
+00:01:34,770 --> 00:01:38,630
+يبقى يعتبر non carbohydrate carbon source بيكون
+
+23
+00:01:38,630 --> 00:01:43,250
+على شكل sodium سيتريات في بعض أنواع البكتيريا
+
+24
+00:01:43,250 --> 00:01:47,350
+قادرة على استهلاك ال sodium سيتريات و الحصول على
+
+25
+00:01:47,350 --> 00:01:52,310
+carbonلازم لنموء البكتيريا وينقسمها لكن في بعض
+
+26
+00:01:52,310 --> 00:01:55,250
+أنواع البكتيريا ما عندها القدرة على استهلاك
+
+27
+00:01:55,250 --> 00:01:59,870
+السيترات طب كيف بتكون السيترات داخل الخلية؟ زي ما
+
+28
+00:01:59,870 --> 00:02:03,750
+قلنا في الكتاليز الـ hydrogen peroxide بتكون خلال
+
+29
+00:02:03,750 --> 00:02:09,170
+عملية التنفس الخلوي السيترات لازم يكون انتلوديات
+
+30
+00:02:09,170 --> 00:02:14,550
+في إحدى العمليات الأيضيةعشان يصلّه استهلاك بقولّي
+
+31
+00:02:14,550 --> 00:02:17,870
+أن السيطرات ال Sodium citrate بيكون intermediate
+
+32
+00:02:17,870 --> 00:02:22,970
+في ال crib cycle في ال crib cycle يبقى ال Sodium
+
+33
+00:02:22,970 --> 00:02:26,270
+citrate هو ال substrate اللي هيشتغل عليه ال enzyme
+
+34
+00:02:26,270 --> 00:02:30,910
+بيكون في ال in vivo intermediate في ال crib cycle
+
+35
+00:02:30,910 --> 00:02:36,430
+بالنسبة للتفاعل كيف هتستهلك البكتيريا ال Sodium
+
+36
+00:02:36,430 --> 00:02:40,630
+citrate بقولّي أن انزيمات السيطرات تعتبر
+
+37
+00:02:40,630 --> 00:02:45,130
+endoenzymeعشان البكتيريا تقدر تستهلك الـ Sodium
+
+38
+00:02:45,130 --> 00:02:48,830
+Citrate لازم يكون عند البكتيريا انزيم الـ Citrate
+
+39
+00:02:48,830 --> 00:02:54,870
+لازم هذا الانزيم هيكسر Sodium Citrate اللي اتكون
+
+40
+00:02:54,870 --> 00:03:00,330
+في الـ Crepe Cycle هتحول Sodium Citrate لـ
+
+41
+00:03:00,330 --> 00:03:05,100
+Oxaloacetic Acid زائد أسيتاتكمان البكتيريا اللى
+
+42
+00:03:05,100 --> 00:03:08,380
+عندها القدرة أو اللى عندها انزيم استيتريات لياز في
+
+43
+00:03:08,380 --> 00:03:12,360
+عندها انزيم الـ oxaloacetic acid decarboxylase
+
+44
+00:03:12,360 --> 00:03:17,640
+هتنزع الـ CO2 الكربون من ال oxaloacetic acid
+
+45
+00:03:17,640 --> 00:03:22,060
+هيتكون عندى بيروفات البيروفات هو ال carbon source
+
+46
+00:03:22,060 --> 00:03:27,500
+هو ال carbon source اللى لازم لنمو البكتيريا
+
+47
+00:03:27,500 --> 00:03:31,550
+وانقسمهاأحنا قلنا إن الـ oxaloacetic acid هيعمل
+
+48
+00:03:31,550 --> 00:03:35,910
+decarboxylase هينزع الـ CO2 في عندي الصوديوم
+
+49
+00:03:35,910 --> 00:03:39,210
+الصوديوم الـ excess من الصوديوم مع الـ CO2 هيتكون
+
+50
+00:03:39,210 --> 00:03:45,910
+عندي Na2CO3 هذا يعتبر alkaline فبالتالي هيرفعلي
+
+51
+00:03:45,910 --> 00:03:50,810
+الـ pH يبقى صار عندي مصدر كربون من non
+
+52
+00:03:50,810 --> 00:03:55,820
+carbohydrate carbon source من الـ citrateبقول لي
+
+53
+00:03:55,820 --> 00:03:59,200
+البكتيريا اللى عندها القدرة على استهلاك الـ Sodium
+
+54
+00:03:59,200 --> 00:04:03,420
+Citrate إلى جانب استهلاك هلاء الـ Citrate عندها
+
+55
+00:04:03,420 --> 00:04:09,020
+القدرة على استهلاك الـ Ammonia الـ NH4 Plus عندها
+
+56
+00:04:09,020 --> 00:04:14,740
+القدرة على إنتاجه وتحويله لـ Ammonia هذا يعتبر
+
+57
+00:04:14,740 --> 00:04:18,880
+Nitrogen Source يبقى صار عندي مصدر للنيتروجين
+
+58
+00:04:18,880 --> 00:04:23,160
+ومصدر للكربون هيك البكتيريا بتقدر تنمو وتنقسم
+
+59
+00:04:23,160 --> 00:04:28,930
+وتتكعفرهذا بالنسبة لـ In-Vivo طيب In-Vitro عشان
+
+60
+00:04:28,930 --> 00:04:33,870
+أكشف هل البكتيريا عندها أنزيم السيطرات لياز أو لأ
+
+61
+00:04:33,870 --> 00:04:38,790
+لازم أنا يكون في ال media Sodium Citrate هي
+
+62
+00:04:38,790 --> 00:04:42,570
+السبسترات اللي هيشتغل عليها الانزال يبقى In-Vitro
+
+63
+00:04:42,570 --> 00:04:46,750
+عشان أكشف عن وجود الانزيم بقوللي بتحط في ال media
+
+64
+00:04:46,750 --> 00:04:50,650
+Sodium Citrate السبسترال عشان يشتغل عليها الانزيم
+
+65
+00:04:50,650 --> 00:04:56,630
+هذه موجودة في ال media طيبالانزيم السيطرية تلياز
+
+66
+00:04:56,630 --> 00:05:00,370
+قلنا يعتبر اندو انزايم التفاعل بيصير intra
+
+67
+00:05:00,370 --> 00:05:03,910
+cellular داخل الخلية ال Sodium سيطرية حجمها مش
+
+68
+00:05:03,910 --> 00:05:08,850
+كبير ال substrate حجمها مش كبير بالتالي ال انزيم
+
+69
+00:05:08,850 --> 00:05:13,130
+او ال substrate بما انها موجودة في ال media يبقى
+
+70
+00:05:13,130 --> 00:05:18,090
+خارج الخلية بقولنا لازم تدخل لداخل البكتيريا احنا
+
+71
+00:05:18,090 --> 00:05:21,850
+قلنا لو كانتلو كان الانزيم اندو انزايم ممكن
+
+72
+00:05:21,850 --> 00:05:24,950
+السبسترات يكون داخل الخلية وممكن يكون خارج الخلية
+
+73
+00:05:24,950 --> 00:05:28,250
+إذا كان داخل الخلية كان بيحصل التفاعل داخل الخلية
+
+74
+00:05:28,250 --> 00:05:34,350
+لو كان خارج الخلية البكتيريا هتفرز انزيمة تدخل
+
+75
+00:05:34,350 --> 00:05:38,870
+السبسترات لداخل الخلية بقول لها البكتيريا هتفرز
+
+76
+00:05:38,870 --> 00:05:43,190
+انزيم السيترات بيريماز هذا هيدخل السوديوم سيترات
+
+77
+00:05:43,190 --> 00:05:47,870
+الموجود في الميديا لداخل الخلية تمامهكذا أصبح الـ
+
+78
+00:05:47,870 --> 00:05:52,450
+substrate موجود داخل الخلية نكمل الخطوات نفس اللي
+
+79
+00:05:52,450 --> 00:05:57,910
+ذكرناها بالـ in vivoهيجي السيطرات لياز يكسر الـ
+
+80
+00:05:57,910 --> 00:06:01,510
+Sodium سيطرات الموجود داخل الخلية سار هيكسره
+
+81
+00:06:01,510 --> 00:06:05,990
+لأجزاله Acetic acid و Acetate هيجي الأجزاله Acetic
+
+82
+00:06:05,990 --> 00:06:09,930
+acid دي كربوكسلاز هينزع الـ CO2 يتكون بيروفات هو
+
+83
+00:06:09,930 --> 00:06:14,810
+ال carbon source هيظل عندي excess of Sodium مع الـ
+
+84
+00:06:14,810 --> 00:06:19,010
+CO2 هيصير في عندي alkaline product هيرفعلي الـ pH
+
+85
+00:06:19,010 --> 00:06:22,450
+إلى جانب استهلاكها لأس Sodium سيطرات هيتم
+
+86
+00:06:22,450 --> 00:06:27,210
+استهلاكها الـ NH4 plus هتتحول لأمونيا تعتبر ك
+
+87
+00:06:27,210 --> 00:06:31,770
+نيتروجين source يبقى هاي ال principle الفحص تكون
+
+88
+00:06:31,770 --> 00:06:34,970
+عندي بالنهاية ال biofat اللي هو ال carbon source
+
+89
+00:06:34,970 --> 00:06:40,910
+والأمونيا ال nitrogen source citrate utilization
+
+90
+00:06:40,910 --> 00:06:45,970
+test for the ability of bacteria to convertCytrate
+
+91
+00:06:45,970 --> 00:06:50,890
+into oxaloacetate بقول لي هو هذا الفحص بدي أكشف عن
+
+92
+00:06:50,890 --> 00:06:54,690
+قدرة البكتيري عن تحويل الـCytrate تمام اللي هو
+
+93
+00:06:54,690 --> 00:06:57,870
+يعتبر intermediate في الـ Krebs Cycle ل
+
+94
+00:06:57,870 --> 00:07:01,450
+Oxaloacetate برضه intermediate آخر في ال Krebs
+
+95
+00:07:01,450 --> 00:07:05,260
+Cycleهيتحول لـ Oxaloacetate وبعدين التفاعل اللي
+
+96
+00:07:05,260 --> 00:07:10,540
+قلنا بيتحول لبيروفات لمصدر للـ CARB هذا الاختبار
+
+97
+00:07:10,540 --> 00:07:14,000
+استخدم للتفارق بين مجموعات العائلة الـ
+
+98
+00:07:14,000 --> 00:07:18,260
+Enterobacteraceae هذه الفقرة هي المهمة لهذا الفحص
+
+99
+00:07:18,260 --> 00:07:21,960
+هذا الفحص يفرق بين مين ومين بقول هذا الفحص يفرق
+
+100
+00:07:21,960 --> 00:07:27,200
+مجموعات الـ Enterobacteraceae it is selective for
+
+101
+00:07:27,200 --> 00:07:31,570
+bacteria that hasthe ability to consume citrate as
+
+102
+00:07:31,570 --> 00:07:34,850
+its sole source of the carbon بقولّي هذه ال media
+
+103
+00:07:34,850 --> 00:07:38,830
+ال citrate هيتعرف عليها كمان شوية هي selective
+
+104
+00:07:38,830 --> 00:07:44,450
+media اتعرفنا قبل في المعمل السابق على أنواع ال
+
+105
+00:07:44,450 --> 00:07:47,970
+media حسب ال function قسمناها إلى general
+
+106
+00:07:47,970 --> 00:07:51,670
+selective differential transport ال selective قلنا
+
+107
+00:07:51,670 --> 00:07:56,650
+بتنمي نوع معين من البكتيريا ال media ال citrate
+
+108
+00:07:56,650 --> 00:08:00,460
+فيها فقطالسيطرات كمصدر
+
+109
+00:08:28,880 --> 00:08:34,600
+استهلاك السيطرات هتكسر السيطرات هتحصل على بيروفيت
+
+110
+00:08:34,600 --> 00:08:37,420
+هيكون هو ال carbon source اللازم لنمو البكتيريا
+
+111
+00:08:37,420 --> 00:08:41,360
+يبقى اللي فقط هتنمو على هذه ال media هي اللي عندها
+
+112
+00:08:41,360 --> 00:08:44,660
+القدرة على استهلاك السيطرات اللي ماعندها القدرة
+
+113
+00:08:44,660 --> 00:08:47,780
+على استهلاك السيطرات اللي ماعندها انتمات السيطرات
+
+114
+00:08:47,780 --> 00:08:51,560
+لياز ماهتنمو في هذه ال media عشان هيك قال لي
+
+115
+00:08:51,560 --> 00:08:55,720
+selective some intestinal bacteria are positive
+
+116
+00:08:55,720 --> 00:09:00,570
+سيطرات بقول لي بعضالبكتيريا الانتستينال بتكون
+
+117
+00:09:00,570 --> 00:09:03,730
+positive لهذا الفحص مثلًا كالبسيلة، الستروبكتر،
+
+118
+00:09:03,730 --> 00:09:08,110
+الانتيروبكتر لكن ال negative اللي هي ال E coli
+
+119
+00:09:08,110 --> 00:09:14,190
+يبقى هذا هو ال significant لهذا الفحص خلّينا بنشوف
+
+120
+00:09:14,190 --> 00:09:19,520
+هذا ال significant لهذا الفحصبقولنا لو عملنا
+
+121
+00:09:19,520 --> 00:09:22,480
+جرامستان و طلعت جرام نيجاتيب بس ال life يعني دي
+
+122
+00:09:22,480 --> 00:09:26,620
+أكتر من احتمال ممكن يكون صدوموناس انتيرو بكترياسي
+
+123
+00:09:26,620 --> 00:09:33,440
+فيبريو كوليرة أسينيتوباكتر هنتعرف في الفحص
+
+124
+00:09:33,440 --> 00:09:37,340
+الأكسداس كيف نفرق منهم هلأ لو طلعت البكتيريا
+
+125
+00:09:37,340 --> 00:09:41,060
+انتيرو بكترياسي بقولي انتيرو بكترياسي تقسم إلى
+
+126
+00:09:41,060 --> 00:09:45,850
+مجموعتينالكوليفارم أو بسميها ال lactose fermenter
+
+127
+00:09:45,850 --> 00:09:51,050
+الكوليفارم هي بكتيريا موجودة في الأمعاء ال non
+
+128
+00:09:51,050 --> 00:09:55,170
+-coliform أو ال non-lactose fermenter بس المجموعة
+
+129
+00:09:55,170 --> 00:09:58,110
+التانية ال non-coliform أو بسميها ال non-lactose
+
+130
+00:09:58,110 --> 00:10:02,210
+fermenter نيجي لمجموعة الكوليفارم اللاكتوز فرمنتر
+
+131
+00:10:02,210 --> 00:10:07,150
+بقول يتقسم برضه لاسمين فيكل اللي هي مصدرها ال
+
+132
+00:10:07,150 --> 00:10:09,410
+stool الفيكل
+
+133
+00:10:11,230 --> 00:10:14,590
+كوليفارم اللي هي الـ E coli و الـ Klebsiella لكن
+
+134
+00:10:14,590 --> 00:10:17,530
+الـ non-fecal اللي هي مصدرها مش الستول زي ورق
+
+135
+00:10:17,530 --> 00:10:21,570
+الشجر الـ Enterobacter و الستروبكتر لو عملت فحص
+
+136
+00:10:21,570 --> 00:10:26,530
+السيطرات و لفحص السيطرات في الكوليفارم الـ E coli
+
+137
+00:10:26,530 --> 00:10:30,450
+negative لكن باقي ال Klebsiella ال Enterobacter و
+
+138
+00:10:30,450 --> 00:10:35,270
+الستروبكتر يعتبروا positive في فحص السيطرات ذكرنا
+
+139
+00:10:35,270 --> 00:10:41,090
+سابقا فحص ال motilityكلهم motile معادلة الكيبسيلا
+
+140
+00:10:41,090 --> 00:10:44,870
+non motile يبقى عندي فحصين للتفريق بين الكوليفار
+
+141
+00:10:44,870 --> 00:10:47,970
+من ال lactose fermenter السيطرات و ال motility
+
+142
+00:10:47,970 --> 00:10:51,570
+عشان
+
+143
+00:10:51,570 --> 00:10:56,670
+أكشف عن هل البكتيري عندها أنزيم السيطرات لياز أو
+
+144
+00:10:56,670 --> 00:11:00,330
+لأ عندها القدرة على استهلاك السيطرات ولا لأ بقولي
+
+145
+00:11:00,330 --> 00:11:04,810
+في عندي طريقتين الطريقة الأولى اسمها Simons method
+
+146
+00:11:04,810 --> 00:11:09,930
+الطريقة التانية اسمها Causars methodالسيمونز ميثود
+
+147
+00:11:09,930 --> 00:11:14,550
+بقولي بستخدم ميديا اسمها simon citrate agar هي
+
+148
+00:11:14,550 --> 00:11:20,030
+الميديا انا بحضرها بعد ما انا احضرها ب .. بعمل ..
+
+149
+00:11:20,030 --> 00:11:24,690
+ب .. دوبها على ال hot plate بعد ما الدوب بصوبها في
+
+150
+00:11:24,690 --> 00:11:27,990
+تيوب بدخلها ال autoclave تتعقم بعد ما تطلع من ال
+
+151
+00:11:27,990 --> 00:11:32,090
+autoclave ال autoclave بدي اخليها تتصلب بزاوية
+
+152
+00:11:32,090 --> 00:11:36,670
+بديها تكون slant تمام؟بعد ما تتصلب في زاوية بتكون
+
+153
+00:11:36,670 --> 00:11:41,470
+جاهزة للاستخدام بقول لي عشان تكشفي عن قدرة
+
+154
+00:11:41,470 --> 00:11:45,010
+البكتيريا عن استهلاك السايتريات أو لأ بتجيبي
+
+155
+00:11:45,010 --> 00:11:49,370
+السيمون سايتريات أجار وبتاخدي loop full of بكتيريا
+
+156
+00:11:49,370 --> 00:11:53,910
+بتعملي كيف أنا بزرع ال slug بعمل stabbing بعدين
+
+157
+00:11:53,910 --> 00:11:58,090
+بزرع دي جزارة بعد ما أزرع البكتيريا اللي بدي أكشف
+
+158
+00:11:58,090 --> 00:12:01,290
+قدرتها على استهلاك السايتريات أو لأ بعمل
+
+159
+00:12:01,290 --> 00:12:08,240
+incubation لمدة 24 ساعة37 درجة سليسيا صح بقول لي
+
+160
+00:12:08,240 --> 00:12:13,380
+لو نمت البكتيريا واتحول اللون للون الأزرق يبقى هي
+
+161
+00:12:13,380 --> 00:12:17,400
+positive للفحص لكن لو ما نمت البكتيريا واتحول
+
+162
+00:12:17,400 --> 00:12:21,060
+اللون لللون أو ضلت ال media لونها أخضر لون ال
+
+163
+00:12:21,060 --> 00:12:24,360
+media لونها أخضر لو ضل ال media لونها أخضر يبقى هي
+
+164
+00:12:24,360 --> 00:12:29,080
+negative لهذا الفحص عشان نفهم أكترليش اتحول الـ
+
+165
+00:12:29,080 --> 00:12:32,180
+Loan و ليش في عندي Growth في النتيجة الـ Positive
+
+166
+00:12:32,180 --> 00:12:36,220
+بإننا نتعرف على مكونات الـ Media بما إنها أجار
+
+167
+00:12:36,220 --> 00:12:40,820
+بقولنا نسبة الأجار فيها بيكون 1.5% في عندي Sodium
+
+168
+00:12:40,820 --> 00:12:45,180
+Citrate و NH4 Plus كSubstrate اللي هيشتغل عليهم
+
+169
+00:12:45,180 --> 00:12:49,320
+الانزيم الـ Citrate عشان أحصل على Carbon و
+
+170
+00:12:49,320 --> 00:12:53,060
+Nitrogen Source مافي عندي أي مصدر آخر للكربون
+
+171
+00:12:53,060 --> 00:12:55,740
+والنيترجن مافي عندي Beef Extract ولا بيبتون
+
+172
+00:12:55,740 --> 00:13:00,420
+الموجودين في أي Media فقطموجود Sodium Citrate و N
+
+173
+00:13:00,420 --> 00:13:04,480
+-H4 Plus هما اللي هيكونوا المصدر الوحيد لل Carbon
+
+174
+00:13:04,480 --> 00:13:08,920
+و ال Nitrogen NACL اللي هو ال Normal Saline أي
+
+175
+00:13:08,920 --> 00:13:13,120
+ميديا هيكون فيها ال NACL عشان تحافظ على ال Osmotic
+
+176
+00:13:13,120 --> 00:13:17,760
+إبرادشار فهذه الميديا فيها عندى BH Indicator ال BH
+
+177
+00:13:17,760 --> 00:13:23,220
+Indicator اسمه Bromothymol Blue عشان أكشف صار فيا
+
+178
+00:13:23,220 --> 00:13:27,660
+عندى التفاعل ولا لأالبرامو ثيمال بلو حدوده ان عندي
+
+179
+00:13:27,660 --> 00:13:32,360
+انا من ال 96 ل 76 بيكون ال neutral بيكون اللون
+
+180
+00:13:32,360 --> 00:13:37,240
+اللون إجرين يبقى
+
+181
+00:13:37,240 --> 00:13:42,900
+لما يكون ال bh ما بين 96 و 76 بيكون اللون ال media
+
+182
+00:13:42,900 --> 00:13:47,800
+إجرين بقول لو كان أقل من 96 ال bh بيكون بتحول
+
+183
+00:13:47,800 --> 00:13:51,900
+اللون للون yellow لو كان أعلى من 76 بيكون ال
+
+184
+00:13:51,900 --> 00:13:57,040
+alkalineبتحول اللون للون e-blue احنا قولنا لو
+
+185
+00:13:57,040 --> 00:14:02,120
+البكتيريا عملت استهلاك لل Sodium citrate Sodium
+
+186
+00:14:02,120 --> 00:14:07,390
+citrate هو المصدر الوحيد لل carbon فتستهلكهتعمل
+
+187
+00:14:07,390 --> 00:14:11,750
+تكسير استهلاك للسيدرات هيتحول السيدرات ل biofat ال
+
+188
+00:14:11,750 --> 00:14:15,870
+biofat هو المصدر الوحيد لقى الكربون إلى جانب
+
+189
+00:14:15,870 --> 00:14:19,830
+استهلاك هلاقى ال sodium سيدرات هتستهلك ال N-H4
+
+190
+00:14:19,830 --> 00:14:24,450
+plus هتحوله ل أمونيا هيعتبر ك nitrogen source
+
+191
+00:14:24,450 --> 00:14:27,690
+فبالتالي صار عندى carbon source و nitrogen source
+
+192
+00:14:27,690 --> 00:14:33,090
+هتنمو البكتيريا و تتكاثر طبعا انا عندى اتكوين
+
+193
+00:14:33,090 --> 00:14:38,480
+الأمونيا ال N-Hالـ NH3 يعتبر alkaline فبالتالي
+
+194
+00:14:38,480 --> 00:14:43,560
+هيرفع لي ال BH ويتحول لون ال media للون blue احنا
+
+195
+00:14:43,560 --> 00:14:46,840
+قلنا ان لون ال media green في الأصل قبل ما انا
+
+196
+00:14:46,840 --> 00:14:52,540
+ازرع صار في عندي ارتفاع في ال BH صار ال BH عندي
+
+197
+00:14:52,540 --> 00:14:59,500
+alkaline يتحول لون ال media للون blueهذا المخطط
+
+198
+00:14:59,500 --> 00:15:04,260
+يبقى هيكون في عندى نمو للبكتيريا لأنه في عندها صار
+
+199
+00:15:04,260 --> 00:15:08,480
+مصدر لل carbon ومصدر لل nitrogen اللي هم ال sodium
+
+200
+00:15:08,480 --> 00:15:13,800
+citrate و ال N-H4 plus اتحول لوان الوسط للوان blue
+
+201
+00:15:13,800 --> 00:15:16,740
+لأنه اتكون عندى ال ammonia ال ammonia تعتبر
+
+202
+00:15:16,740 --> 00:15:19,980
+alkaline كمان قلنا في تفاعل ال sodium citrate
+
+203
+00:15:19,980 --> 00:15:24,490
+اتكون عندىبالتالي إكسس من النا من الصوديوم مع ال
+
+204
+00:15:24,490 --> 00:15:30,490
+CO2 بتكون عندي NA2CO3 هذا ال alkaline فبالتالي
+
+205
+00:15:30,490 --> 00:15:35,590
+برضه هيرفعلي ال pH لكن لو البكتيريا ما عندها انزيم
+
+206
+00:15:35,590 --> 00:15:39,190
+السيترات لياز أو ماعندها القدرة على استهلاك
+
+207
+00:15:39,190 --> 00:15:39,970
+السيترات
+
+208
+00:15:42,250 --> 00:15:45,950
+البكتيريا ماعندها كمان مصادر اخرى لكربون او
+
+209
+00:15:45,950 --> 00:15:49,010
+نيتروجين مصادر اخرى مافي عندى في ال media اي مصادر
+
+210
+00:15:49,010 --> 00:15:53,050
+اخرى مافي عندى beef extract ولا ببطون فبالتالي
+
+211
+00:15:53,050 --> 00:15:57,640
+البكتيريا مش هتنمو مش هيكون في عندي growthالـ ما
+
+212
+00:15:57,640 --> 00:16:01,420
+تكون عندي أي product alkaline يبقى يظل الوسط
+
+213
+00:16:01,420 --> 00:16:05,020
+agreed فيه لـ neutral يبقى هذا تفسير النتائج في
+
+214
+00:16:05,020 --> 00:16:08,380
+الـ positive بكون عندي growth زائد اللون اللون
+
+215
+00:16:08,380 --> 00:16:13,400
+blue لكن في الـ negative no growth مع بقاء ال
+
+216
+00:16:13,400 --> 00:16:18,280
+media لونها agreed بقولي لو صار عندي لون أزرقلكن
+
+217
+00:16:18,280 --> 00:16:22,800
+مع وجود no growth مافي عندى اى نمو بقوللى هان بكون
+
+218
+00:16:22,800 --> 00:16:26,640
+خلل فى ال media سواء فى التخزين او انها انتهت فترة
+
+219
+00:16:26,640 --> 00:16:31,780
+صلاحيتها هذا بالنسبة ل ال Simon method اللى بستخدم
+
+220
+00:16:31,780 --> 00:16:35,280
+فيها Simon سيطرات اجار بالنسبة لطريقة ال Quasar
+
+221
+00:16:35,280 --> 00:16:40,440
+method بستخدم Quasar سيطرات بروث و ليس اجار بقوللى
+
+222
+00:16:41,720 --> 00:16:45,340
+هذه ال media بتكون ال broth بحضرها عادي بتكون
+
+223
+00:16:45,340 --> 00:16:49,520
+موجودة بوتيل بحضرها ال broth سائلة بجيب loop full
+
+224
+00:16:49,520 --> 00:16:53,420
+of ال bacteria البكتيريا اللي بتكشف عندها انزيم
+
+225
+00:16:53,420 --> 00:16:56,080
+السيطرات لياز أو لأ انها القدرة على استهلاك
+
+226
+00:16:56,080 --> 00:16:59,780
+السيطرات أو لأ بعمل mix بعمل incubation لمدة 24
+
+227
+00:16:59,780 --> 00:17:04,960
+ساعة على 37 درجة سيليزياإذا كان في عندي نمو يبقى
+
+228
+00:17:04,960 --> 00:17:10,480
+هي positive للفحص إذا مافي عندي نمو يبقى فيه مافي
+
+229
+00:17:10,480 --> 00:17:15,620
+عندي نمو يبقى negative للفحص بالنسبة للنتيجة ال
+
+230
+00:17:15,620 --> 00:17:19,440
+growth ال growth في ال broth قولنا إذا بتتذكروا في
+
+231
+00:17:19,440 --> 00:17:23,180
+معمل ال media preparation قولنا أنواع النمو في ال
+
+232
+00:17:23,180 --> 00:17:29,040
+broth ممكن يكون turbidity أو ممكن يكون pellicle أو
+
+233
+00:17:29,040 --> 00:17:34,070
+ممكن يكون sedimentفبالتالي أي شكل من هذه الأشكال
+
+234
+00:17:34,070 --> 00:17:39,290
+يعتبر أنه في عندي Growth هذا أشكال أنه مافي البروث
+
+235
+00:17:39,290 --> 00:17:42,810
+EQ مكونات ال media بما أنها البروث مافي عندي أجار
+
+236
+00:17:42,810 --> 00:17:47,630
+في عندي Sodium Citrate و NH4 Plus كsubstrate هذه
+
+237
+00:17:47,630 --> 00:17:51,110
+هتكون مصدر للكربون مصدر وحيد للكربون هذه هتكون
+
+238
+00:17:51,110 --> 00:17:56,310
+مصدر وحيد للنيتروجين NACL الـ Normal Saline هو
+
+239
+00:17:57,380 --> 00:18:00,420
+هيحفظلي على الـ Osmotic Pressure مافي عندي BH
+
+240
+00:18:00,420 --> 00:18:05,080
+Indicator في هذه الميديا فبالتالي ما هيكون في عندى
+
+241
+00:18:05,080 --> 00:18:08,900
+اختلاف في اللون فقط إذا وجود النموم هو اللي
+
+242
+00:18:08,900 --> 00:18:14,330
+هيكشفلي عن هذا الانزيميبقى في عندي فروقات بين
+
+243
+00:18:14,330 --> 00:18:18,490
+الطريقتين أول فرق أن هاي الطريقة فيها أجار لكن هاي
+
+244
+00:18:18,490 --> 00:18:22,630
+الطريقة هاي ال media ال causer سيطرات هي ال growth
+
+245
+00:18:22,630 --> 00:18:28,070
+الاختلاف التاني أن هان في عندي pH indicator هان
+
+246
+00:18:28,070 --> 00:18:31,970
+مافي عندي pH indicator هان ال result هيكون في عندي
+
+247
+00:18:31,970 --> 00:18:37,260
+نمو بعد تغير اللون لكن هان فقط هيكون نموطبعاً
+
+248
+00:18:37,260 --> 00:18:42,380
+هتنمو البكتيريا لأنه لو كان عندها أنزيمات أو لو
+
+249
+00:18:42,380 --> 00:18:46,200
+كان عندها القدرة على استهلاك السييتريات هتحول الـ
+
+250
+00:18:46,200 --> 00:18:53,140
+Sodium هتكسر السييتريات لـ Oxaloacetic Acid هيتحول
+
+251
+00:18:53,140 --> 00:18:56,680
+بواسطة أنزيم الـ Oxaloacetic Acid D-Carboxylase
+
+252
+00:18:56,680 --> 00:19:00,780
+لبيهروفات مصدر للكربون لازم لنمو البكتيريا
+
+253
+00:19:00,780 --> 00:19:04,560
+بالإضافة لاستهلاكها للسييتريات هتستهلك الـ N-H4
+
+254
+00:19:04,560 --> 00:19:08,910
+Plus لأمونيافبالتالي صار في عندي مصدر للـ carbon و
+
+255
+00:19:08,910 --> 00:19:13,470
+الـ nitrogen هتنمو البكتيريا و تتكاثر و يكون في
+
+256
+00:19:13,470 --> 00:19:17,710
+عندي turbidity او sediment او pellicle لكن لو كان
+
+257
+00:19:17,710 --> 00:19:20,950
+ما عندها مش هيكون في عندي اي نمو لأن مافي عندي اي
+
+258
+00:19:20,950 --> 00:19:25,370
+مصادر اخرى لل carbon او ال nitrogen هذا بالنسبة
+
+259
+00:19:25,370 --> 00:19:31,590
+لطريقة الكشف عن السيطرات utilization نيجي لالسيمون
+
+260
+00:19:31,590 --> 00:19:35,290
+سايتريات أجار organism that metabolize citrate
+
+261
+00:19:35,290 --> 00:19:38,430
+utilize the ammonium salt releasing ammonia and
+
+262
+00:19:38,430 --> 00:19:42,030
+increasing the pH of the media بقوللي البكتيريا
+
+263
+00:19:42,030 --> 00:19:45,050
+اللي عندها القدرة على استهلاك السايتريات يعني ده
+
+264
+00:19:45,050 --> 00:19:47,850
+القدرة على استهلاك ال ammonium salt اللي هي ال N
+
+265
+00:19:47,850 --> 00:19:51,630
+-H4 plus بالتالي لما تستهلك ال ammonium salt
+
+266
+00:19:51,630 --> 00:19:56,130
+هتكوني الأمانيا الأمانيا هترفعلي ال pH في الميديا
+
+267
+00:19:56,130 --> 00:20:00,120
+في انديبرامو تيمولي بلوموجود في الـ media as
+
+268
+00:20:00,120 --> 00:20:05,080
+indicator زي BH indicator بقول هيحول اللون لـ
+
+269
+00:20:05,080 --> 00:20:10,080
+green لـ neutral BH قولنا إنه اللي فيه ال neutral
+
+270
+00:20:10,080 --> 00:20:15,640
+BH هيكون اللون إجريه بما إن ال BH ارتفع بالتالي
+
+271
+00:20:15,640 --> 00:20:20,760
+هيتحول اللون للون أزرق blue لما يكون أعلى من 7.6
+
+272
+00:20:21,600 --> 00:20:24,960
+سيمون سايتريات أجار بيعرف لسيمون سايتريات أجار
+
+273
+00:20:24,960 --> 00:20:27,940
+بتحتوي على صوديوم سايتريات as a sole carbon source
+
+274
+00:20:27,940 --> 00:20:31,440
+كمصدر واحد للكربون امونيوم ايون كمصدر واحد
+
+275
+00:20:31,440 --> 00:20:35,620
+للنيتروجين and برامو ثمال بلو لو ما كتبت برامو
+
+276
+00:20:35,620 --> 00:20:39,940
+ثمال بلو بتتحول ال media لcausar citrate broth
+
+277
+00:20:39,940 --> 00:20:44,120
+يبقى هذه بتحتوي على مصدر كربون واحد مصدر نيتروجين
+
+278
+00:20:44,120 --> 00:20:49,320
+واحد وبرامو ثمال بلوكيف طريقة الشغل قلنا نستخدم
+
+279
+00:20:49,320 --> 00:20:53,080
+eniculating needle عشان نعمل stabbing بعدين بعمل
+
+280
+00:20:53,080 --> 00:21:01,160
+zigzag بزرع ال slant بيعمل incubation لمدة 24 ساعة
+
+281
+00:21:01,160 --> 00:21:05,920
+على 37 درجة السليسيس بشوف ال growth و التغير في
+
+282
+00:21:05,920 --> 00:21:10,280
+اللون النتيجة ال positive بشوفها بكون في عندي no
+
+283
+00:21:10,280 --> 00:21:14,040
+moreمع تغير في اللون لللون الأزرق والـ growth on
+
+284
+00:21:14,040 --> 00:21:17,820
+the media is combined by a rise in a pH to change
+
+285
+00:21:17,820 --> 00:21:20,800
+the media from its initial green color to deep
+
+286
+00:21:20,800 --> 00:21:25,180
+blue والـ growth هيكون في الميديا وكمان هيكون الـ
+
+287
+00:21:25,180 --> 00:21:30,940
+pH هيتغير في الميديا من green ل blue نلاحظ هنا في
+
+288
+00:21:30,940 --> 00:21:34,620
+عندي control negative control لون الميديا ضاله لون
+
+289
+00:21:34,620 --> 00:21:41,000
+green أخضرالأزرق هنا عندي الـ Enterobacter الـ
+
+290
+00:21:41,000 --> 00:21:44,100
+Enterobacter قولنا positive للسيطرات يبقى هيتحول
+
+291
+00:21:44,100 --> 00:21:47,800
+اللون للون أزرق Equally قولنا negative يبقى هيدل
+
+292
+00:21:47,800 --> 00:21:51,100
+اللون green Eclipsella قولنا positive للسيطرات
+
+293
+00:21:51,100 --> 00:21:55,510
+يبقى هيتحول اللون للون blueالطريقة التانية الـ
+
+294
+00:21:55,510 --> 00:21:59,710
+Causar citrate broth is another medium used to
+
+295
+00:21:59,710 --> 00:22:03,270
+test for citrate utilization طريقة أخرى للكشف على
+
+296
+00:22:03,270 --> 00:22:06,810
+ال citrate utilization Causar citrate broth قلنا
+
+297
+00:22:06,810 --> 00:22:10,690
+is based on this formulation and contains Sodium
+
+298
+00:22:10,690 --> 00:22:13,630
+citrate as a single source of carbon بتحتوي على
+
+299
+00:22:13,630 --> 00:22:17,690
+Sodium citrate كمصدر وحيد للكربون Ammonium salt
+
+300
+00:22:17,930 --> 00:22:21,470
+كمصدر وحيد للنيتروجين مش موجود فيه عندي BH
+
+301
+00:22:21,470 --> 00:22:24,930
+Indicator يبقى هذه الـ Causar Citrate البروث
+
+302
+00:22:24,930 --> 00:22:28,490
+بكتيريا قادرة على استخدام الـ Citrate كمصدر كربون
+
+303
+00:22:28,490 --> 00:22:32,110
+سارس ستنمو في الميديا ويسبب تربيدتي بقول البكتيريا
+
+304
+00:22:32,110 --> 00:22:35,190
+اللي عندها القدرة على استهلاك الـ Citrate كمصدر
+
+305
+00:22:35,190 --> 00:22:38,410
+وحيد للكربون هتنمو في الميديا وهذا هيسبب تربيدتي
+
+306
+00:22:38,410 --> 00:22:41,670
+قلنا مش بس تربيدتي ممكن يكون sediment وممكن يكون
+
+307
+00:22:41,670 --> 00:22:46,550
+pellicle لأنه أشكال النمو في البروث تيوب تربيدتي
+
+308
+00:22:46,550 --> 00:22:51,030
+بيليكل سيدمانالنتيجة الـ Positive Growth كافة ان
+
+309
+00:22:51,030 --> 00:22:54,930
+مو تربدتي زائد sediment زائد pellicle الـ Negative
+
+310
+00:22:54,930 --> 00:22:59,090
+No growth هتكون clear مافي عندي اي تغير في اللون
+
+311
+00:22:59,090 --> 00:23:04,410
+خلصنا من ال citrate utilization يُعتبر endoenzyme
+
+312
+00:23:04,410 --> 00:23:09,210
+نيجي لل Oxidase test ال Oxidase test يُعتبر أو ال
+
+313
+00:23:09,210 --> 00:23:13,780
+Oxidase enzyme أيضا يُعتبر endoenzymeبقول لي the
+
+314
+00:23:13,780 --> 00:23:16,940
+oxidase test is used to identify bacteria
+
+315
+00:23:16,940 --> 00:23:20,220
+containing the respiratory enzyme cytochrome-c
+
+316
+00:23:20,220 --> 00:23:24,280
+oxidase يبقى بدي أكشف هل عند البكتيريا إنزيم الـ
+
+317
+00:23:24,280 --> 00:23:27,940
+cytochrome-c أكسداز ولا لأ اسمه هك لأن ال
+
+318
+00:23:27,940 --> 00:23:30,220
+substrate اللي هيشتغل عليها ال enzyme هي ال
+
+319
+00:23:30,220 --> 00:23:34,480
+cytochrome-c يبقى ال substrate ال cytochrome-c
+
+320
+00:23:34,480 --> 00:23:39,000
+الإنزيم اللي بدي أكشف عليه أكسدازكيف بتكون الـ
+
+321
+00:23:39,000 --> 00:23:42,460
+cytochrome-C؟ بقول الـ cytochrome-C أكسيداز is the
+
+322
+00:23:42,460 --> 00:23:45,540
+terminal enzyme in the electron transport chain
+
+323
+00:23:45,540 --> 00:23:48,780
+بقول لي هو الانزيم النهائي في ال electron
+
+324
+00:23:48,780 --> 00:23:52,960
+transport chain the enzyme which reduce molecular
+
+325
+00:23:52,960 --> 00:23:56,080
+oxygen to water to complete the last link in the
+
+326
+00:23:56,080 --> 00:24:01,480
+chain of aerobic respirationبتكون في نهاية اللي هو
+
+327
+00:24:01,480 --> 00:24:06,600
+عملية التنفس الخلوي في نهاية الـ Krebs Cycle بيحدث
+
+328
+00:24:06,600 --> 00:24:09,900
+Redox Reaction اللي هو Oxidation Reduction
+
+329
+00:24:09,900 --> 00:24:14,780
+Reaction الـ Cytochrome C أكسيداز موجود في الـ
+
+330
+00:24:14,780 --> 00:24:17,500
+Electron Transport Exchange هذا الإنزين بيقول
+
+331
+00:24:17,500 --> 00:24:23,190
+بيعمل Reduce لـ Molecular Oxygen لوطةعشان نفهم
+
+332
+00:24:23,190 --> 00:24:26,590
+أكتر قولنا الـ cytochrome-C أكسداز بيشتغل على الـ
+
+333
+00:24:26,590 --> 00:24:29,510
+substrates اللي هي الـ cytochrome-C أو بسميها
+
+334
+00:24:29,510 --> 00:24:34,670
+reduced cytochrome-C اش يعني reduced يعني مختزل
+
+335
+00:24:34,670 --> 00:24:38,270
+يعني مقتسب إلكترونات الـ cytochrome-C أكسداز من
+
+336
+00:24:38,270 --> 00:24:43,470
+اسمه هيعمل oxidation ل الـ reduced cytochrome-C
+
+337
+00:24:43,470 --> 00:24:47,730
+كانت مكتسبة إلكترونات هتصير فاقدة للإلكترونات
+
+338
+00:24:47,730 --> 00:24:52,920
+هتتحول لأكسداز cytochrome-Cبما أنها فقدت إلكترون
+
+339
+00:24:52,920 --> 00:24:57,720
+يبقى الإلكترون هيروح لإيش تاني لمركب تاني
+
+340
+00:24:57,720 --> 00:25:02,640
+الإلكترون اللي فقدته بقولي هتاخده الـ O2 الـ
+
+341
+00:25:02,640 --> 00:25:06,940
+Molecular Oxygen الـ O2 مع الـ H Plus قولنا في
+
+342
+00:25:06,940 --> 00:25:10,680
+عندي مضخات لترانسبورت الإلكترون الترانسبورت تتشهد
+
+343
+00:25:10,680 --> 00:25:15,880
+مضخات للـ H Plus يبقى عندي H Plus مع الـ Molecular
+
+344
+00:25:15,880 --> 00:25:21,590
+Oxygenفي عندي إلكترون تم فقده خلال عملية الـ
+
+345
+00:25:21,590 --> 00:25:25,410
+Oxidation لـ Cytochrome-C لـ Reduce Cytochrome-C
+
+346
+00:25:25,410 --> 00:25:31,570
+إلكترون زائد H بلس زائد O2 هيتكون عندي H2O يبقى
+
+347
+00:25:31,570 --> 00:25:37,930
+هذا هو ال issue اللي بيصير in vivo in vivo بقولي
+
+348
+00:25:37,930 --> 00:25:42,690
+إن Cytochrome-C Oxidase هيعمل Oxidation لـ Reduce
+
+349
+00:25:42,690 --> 00:25:47,440
+Cytochrome-CReduce يعني مختزل يعني مكتز بإلكترونات
+
+350
+00:25:47,440 --> 00:25:50,640
+بما أنه صار له أكسدة يبقى فقد الإلكترونات صار
+
+351
+00:25:50,640 --> 00:25:54,960
+Oxidized Cytochrome-C الإلكترون وين هيروح؟
+
+352
+00:25:54,960 --> 00:25:59,100
+الإلكترون بقولّي هيرتبط مع الـ Molecular Oxygen مع
+
+353
+00:25:59,100 --> 00:26:02,080
+الـH Plus اللي هي موجود في عندي مدخّات في
+
+354
+00:26:02,080 --> 00:26:04,880
+الإلكترون Transporter تشين هيرتبطوا مع بعض
+
+355
+00:26:04,880 --> 00:26:10,080
+ويكونوني الـH2O يبقى هيك صار في عنده التفاعل in
+
+356
+00:26:10,080 --> 00:26:11,680
+vivo طيب
+
+357
+00:26:19,790 --> 00:26:23,870
+عشان انا اكشف هل عندي انزيم الـ Oxidase ولا لأ
+
+358
+00:26:23,870 --> 00:26:28,570
+بقوللي بجيب Substrate بتشبه الـ Cytochrome-C والله
+
+359
+00:26:28,570 --> 00:26:32,630
+لو عندي انزيم ال Oxidase موجود في البكتيريا هتعمل
+
+360
+00:26:32,630 --> 00:26:37,150
+Oxidation للـ Cytochrome-C هتتحول ل Oxidase
+
+361
+00:26:37,150 --> 00:26:41,170
+Cytochrome-C هتتحول للون تاني بقدر اني انا اكشف
+
+362
+00:26:41,170 --> 00:26:45,910
+عنه هنتعرف هلأ في الـ Principle بقوللي Oxidase
+
+363
+00:26:45,910 --> 00:26:50,970
+producing bacteriaالأمثلة على البكتيريا Oxidase
+
+364
+00:26:50,970 --> 00:26:54,770
+Positive Sodomonas Neisseria Vibrio Aeromonas
+
+365
+00:26:54,770 --> 00:26:59,270
+Sodomonas aeruginosa is a gram negative بكتيريا
+
+366
+00:26:59,270 --> 00:27:02,270
+Sodomonas aeruginosa هي gram negative بس الله
+
+367
+00:27:02,270 --> 00:27:07,450
+aerobic rod هي aerobic هوائية have a strictly
+
+368
+00:27:07,450 --> 00:27:11,410
+respiratory type of metabolismwith oxygen as the
+
+369
+00:27:11,410 --> 00:27:14,370
+terminal electron acceptor and this is أكسداز
+
+370
+00:27:14,370 --> 00:27:17,990
+positive بقوللي إن صلمانوس إيروجينازا هي أكسداز
+
+371
+00:27:17,990 --> 00:27:22,210
+positive طالما أكسداز positive طبيعي إنها تكون
+
+372
+00:27:22,210 --> 00:27:25,870
+obligate aerobic هوائية إجبارية هوائية إجبارية
+
+373
+00:27:25,870 --> 00:27:30,930
+يعني تستخدم الأكسجين كمستقبل نهائي للإلكترونات
+
+374
+00:27:30,930 --> 00:27:39,100
+بالتالي هيصله هيكتسب إلكترون يتحول لH2O هيصيرأكسدة
+
+375
+00:27:39,100 --> 00:27:43,060
+للـ «cytochrome-c» ويتحول لأكسداء الـ «cytochrome
+
+376
+00:27:43,060 --> 00:27:48,560
+-c» لكن الأكسجن هيصله اختزال وهيكتسب إلكترون
+
+377
+00:27:48,560 --> 00:27:53,360
+وهيتحول لـH2Oالانتيرو بكترياسي بقول لي is Oxidase
+
+378
+00:27:53,360 --> 00:27:57,180
+negative bacteria يبقى هي Oxidase negative لكن الـ
+
+379
+00:27:57,180 --> 00:28:01,100
+Sodomonas هي Oxidase positive عشان أفرق الانتيرو
+
+380
+00:28:01,100 --> 00:28:04,040
+بكترياسي عن ال Sodomonas بعمل فحص ال Oxidase لو
+
+381
+00:28:04,040 --> 00:28:07,380
+طلعت positive التنتين طبعا هم إجرام نيجاتيف بصلية
+
+382
+00:28:07,380 --> 00:28:10,760
+Sodomonas و الانتيرو بكترياسي إجرام نيجاتيف بصلية
+
+383
+00:28:11,040 --> 00:28:14,140
+عشان أفرق بينهم بعمل فحص الـ Oxidase إذا طلعت
+
+384
+00:28:14,140 --> 00:28:17,100
+Negative يبقى هي Antirobacteriasy إذا طلعت
+
+385
+00:28:17,100 --> 00:28:21,120
+Positive يبقى هي Sodomonas في فحص تاني اللي هو ال
+
+386
+00:28:21,120 --> 00:28:24,860
+Gelicose Fermenter هنتعرف عليه في ال chapter الجاي
+
+387
+00:28:24,860 --> 00:28:29,720
+Sodomonas aeruginosa هي Non-Gelicose Fermenter لكن
+
+388
+00:28:29,720 --> 00:28:33,300
+ال Antirobacteriasy هي Gelicose Fermenter يبقى
+
+389
+00:28:33,300 --> 00:28:36,880
+هدول الفحصين بيفرقوا لبين ال Antirobacteriasy و
+
+390
+00:28:36,880 --> 00:28:37,820
+Sodomonas
+
+391
+00:28:40,250 --> 00:28:43,910
+كيف انا بدي اكشف عن هذا الانزيم in vitro قلنا
+
+392
+00:28:43,910 --> 00:28:47,410
+بنجيب substrate تشبه ال substrate اللي بيشتغل
+
+393
+00:28:47,410 --> 00:28:51,530
+عليها ال enzyme ال Oxidase test ممكن يكون فيه اندي
+
+394
+00:28:51,530 --> 00:28:55,630
+reagent ممكن يكون strip و ممكن يكون desk بقولي ال
+
+395
+00:28:55,630 --> 00:28:58,130
+reagent او ال substrate اللي هيشتغل عليها ال
+
+396
+00:28:58,130 --> 00:29:03,870
+enzyme ممكن يكون تترا مثال كينايل امين داي امين
+
+397
+00:29:03,870 --> 00:29:11,670
+داي هيدرو كلوراي اوأماينو داي مثل الألانين يبقى ها
+
+398
+00:29:11,670 --> 00:29:15,650
+هي ال substrate اللى هى يشتغل عليها ال enzyme عندى
+
+399
+00:29:15,650 --> 00:29:19,810
+انا بدي اكشف عن ال cytochrome c أكسيداز وقوللى أن
+
+400
+00:29:19,810 --> 00:29:24,110
+cytochrome c أكسيداز هيشتغل على substrate شبيه
+
+401
+00:29:24,110 --> 00:29:28,410
+بالcytochrome c هى هى ال substrate تترا مثال
+
+402
+00:29:28,410 --> 00:29:34,370
+فينايلDiamine هي الـ Substrate اللي هيشتغل عليها
+
+403
+00:29:34,370 --> 00:29:38,770
+الإنزايم شبيهة بـ Cytochrome C Reduced Cytochrome
+
+404
+00:29:38,770 --> 00:29:43,990
+C شبيهة فيها هيشتغل عليها أنزيم الـ Oxidase هاي
+
+405
+00:29:43,990 --> 00:29:47,550
+بتكون مكتسبة إلكترون بقول ليه بيكون لونها
+
+406
+00:29:47,550 --> 00:29:51,290
+Colorless لو موجود عند الإنزيم هيصير Oxidation
+
+407
+00:29:51,290 --> 00:29:57,490
+هيصير في عندي Oxidationهتفقد electron هيفقد هتفقد
+
+408
+00:29:57,490 --> 00:30:01,830
+electron و هتصير كانت هي hand reduced هتصير
+
+409
+00:30:01,830 --> 00:30:06,070
+oxidized بقوللي لما تكون oxidized بتحول لونها للون
+
+410
+00:30:06,070 --> 00:30:10,610
+purple يبقى لما تكون reduced بتكون لونها colorless
+
+411
+00:30:10,610 --> 00:30:15,390
+لكن لما تكون oxidized تكون لونها purple اذا ملاحظ
+
+412
+00:30:15,390 --> 00:30:19,060
+في الصورة كان في عندي hand رابطةصار في عندي رابطة
+
+413
+00:30:19,060 --> 00:30:22,900
+ثنائية يبقى أنا فقدت إلكترون هذا الإلكترون راح
+
+414
+00:30:22,900 --> 00:30:26,800
+للأكسجن للمستقبل النهائي للإلكترونات مع الـ H بلس
+
+415
+00:30:26,800 --> 00:30:31,200
+الموجود في الإلكترون الترانسبورت جان وكان والي الـ
+
+416
+00:30:31,200 --> 00:30:35,480
+water فبالتالي هك أنا بقدر أكشف على الـ cytochrome
+
+417
+00:30:35,480 --> 00:30:39,940
+C أكسيداز إذا أتحول اللون للون purple يبقى النتيجة
+
+418
+00:30:39,940 --> 00:30:43,660
+positive في عندي إنزيم الأكسيداز لأنهالـ
+
+419
+00:30:43,660 --> 00:30:50,360
+cytochrome-C يتقلل ولكن الـ Oxidized بيكون لونه
+
+420
+00:30:50,360 --> 00:30:55,740
+باربر كيف طريقة الشغل بقولي بتاخدي كولوني
+
+421
+00:30:55,740 --> 00:31:00,020
+للبكتيريا اللى بدك تكشف عندها انزيم ال Oxidized
+
+422
+00:31:00,020 --> 00:31:04,600
+ولا لأ او wooden toothpick بقولي في نفس اللى
+
+423
+00:31:04,600 --> 00:31:08,800
+بنعملهم او اللى هم نكشات لصناعة طبعا wooden
+
+424
+00:31:08,800 --> 00:31:13,170
+toothpick ليش بقولي لازم تستخدميوودون توث بيك أو
+
+425
+00:31:13,170 --> 00:31:19,170
+تستخدمي بلاستيك لوب ممنوع تستخدمي الـ Necrum Loop
+
+426
+00:31:19,170 --> 00:31:24,450
+لأن الـ Reagent ممكن يتفاعل مع الـ metal لو أنا
+
+427
+00:31:24,450 --> 00:31:28,150
+استخدمت Necrum Loop الـ Reagent يتفاعل مع الـ
+
+428
+00:31:28,150 --> 00:31:31,310
+metal فبالتالي بيعطيني false positive result عشان
+
+429
+00:31:31,310 --> 00:31:36,110
+هيك بستخدم اما بلاستيك لوب أو وودون توث بيك بقولي
+
+430
+00:31:36,110 --> 00:31:40,030
+بتاخدي هاي البكتيريا و بتحطيها على ال Oxidase Test
+
+431
+00:31:40,220 --> 00:31:44,100
+هذا الـ slide اسمه فيان دي أكسداز تستسلايد بتكون
+
+432
+00:31:44,100 --> 00:31:49,740
+مغمورة بالرياجينت بالأكسداز الرياجينت الموجود في
+
+433
+00:31:49,740 --> 00:31:54,640
+السبسترات اللي هو اللي قلناه قبل شوية الـ
+
+434
+00:31:54,640 --> 00:31:58,490
+tetramethyl phenylDiamine هذه هي الـ Substrate
+
+435
+00:31:58,490 --> 00:32:04,950
+بتكون مغمورة بالـ Oxidase test slide بتكون مغمورة
+
+436
+00:32:04,950 --> 00:32:08,870
+فيها بقولّي ال reaction بتتحول للون purple to
+
+437
+00:32:08,870 --> 00:32:11,550
+appear in the presence of cytochrome oxidase لو
+
+438
+00:32:11,550 --> 00:32:14,570
+عندي ال enzyme هيتحول اللون للون purple لأن ال
+
+439
+00:32:14,570 --> 00:32:17,710
+substrate لونها colorless لما تصير في حالة
+
+440
+00:32:17,710 --> 00:32:22,390
+oxidized لما تصير لها oxidation بتتحول لونها للون
+
+441
+00:32:22,390 --> 00:32:26,960
+purpleواللي بلاحظ تغير اللون بشكل يعني بشكل
+
+442
+00:32:26,960 --> 00:32:30,620
+انتقالي بيتحول اللون أول شيء من pink ل maroon
+
+443
+00:32:30,620 --> 00:32:34,900
+بعدين بتتحول للون purple بقرأ النتيجة خلال عشرين
+
+444
+00:32:34,900 --> 00:32:39,840
+ثانية لازم أني أنا أقرأ النتيجة خلال عشرين ثانية
+
+445
+00:32:39,840 --> 00:32:43,620
+لأن ممكن بعدهاك ال reagent يتأثر بالأكسجين الموجود
+
+446
+00:32:43,620 --> 00:32:46,840
+بالهواء ويعطيني false positive result و اللي في
+
+447
+00:32:46,840 --> 00:32:50,740
+عندي طريقة بديلة أني أنا بجيب ال oxidase reagent
+
+448
+00:32:50,740 --> 00:32:56,220
+ال reagent اللي هيمحضر بيكون و بديروه بشكل مباشر
+
+449
+00:32:56,220 --> 00:32:59,600
+على الكلون الموجودة في الأجار لو اتحول اللون للون
+
+450
+00:32:59,600 --> 00:33:03,640
+بيربول يبقى النتيجة بتكون positive ملاحظاتها ما
+
+451
+00:33:03,640 --> 00:33:06,880
+بقول لي pick your iniculum not with a metal loop و
+
+452
+00:33:06,880 --> 00:33:09,960
+لممنوع تستخدم ال metal loop لازم تستخدم ال plastic
+
+453
+00:33:09,960 --> 00:33:16,020
+loop أو الودن الودن الودن توف pick ليش لأن ال
+
+454
+00:33:16,020 --> 00:33:18,840
+reaction ممكن يتفاعل مع ال metal و يعطيني false
+
+455
+00:33:18,840 --> 00:33:26,020
+positive resultنبقى الحرارة مفرومة أو غير مفتوحة
+
+456
+00:33:26,020 --> 00:33:29,830
+في الحجم حتى تكون محتاجةold reagent is sitting out
+
+457
+00:33:29,830 --> 00:33:33,010
+on the bench and is bearable ask for a new tube
+
+458
+00:33:33,010 --> 00:33:37,350
+for the instructor بقوللي ال reagent اللي
+
+459
+00:33:37,350 --> 00:33:43,370
+بتستخدميه بتخليه بالتلاجة بتخليه مسكر ممنوع تخليه
+
+460
+00:33:43,370 --> 00:33:49,130
+مفتوح فقط عند الحاجة بتستخدميه لو كان old reagent
+
+461
+00:33:49,130 --> 00:33:53,810
+ممنوع انك تستخدميه لأنه ممكن يتغير مع العوامل
+
+462
+00:33:53,810 --> 00:33:58,170
+الخارجية ويعطيني false resultضروري انا اي فحص
+
+463
+00:33:58,170 --> 00:34:01,070
+كيميائي استخدم young culture او fresh culture
+
+464
+00:34:01,070 --> 00:34:04,110
+ممنوع انا استخدم all culture لأن الانزيم activity
+
+465
+00:34:04,110 --> 00:34:08,130
+بقل مع الوقت قلنا fresh reagent لازم انا استخدم ال
+
+466
+00:34:08,130 --> 00:34:12,130
+reagent يكون fresh اخده من ال freezer ممنوع يكون
+
+467
+00:34:12,130 --> 00:34:16,090
+open مفتوح في ال tube لأن قلنا ال reagent ممكن
+
+468
+00:34:16,090 --> 00:34:19,850
+يتغير مع العوامل الخارجية ويعطيني false positive
+
+469
+00:34:19,850 --> 00:34:24,490
+result بقرأ النتيجة خلال عشرين second not after
+
+470
+00:34:24,490 --> 00:34:29,130
+ليش لأنهممكن أن الأكسجين يتفاعل مع الـreagent
+
+471
+00:34:29,130 --> 00:34:32,130
+ويعطيني false positive result عشان هيك بقرأ
+
+472
+00:34:32,130 --> 00:34:38,330
+النتيجة خلال 20 ثانية موريكزيلا كاتريلز and
+
+473
+00:34:38,330 --> 00:34:41,010
+نيسيريا are a gram negative to double كُكّا
+
+474
+00:34:41,010 --> 00:34:43,430
+يتعرفنا على الـgram negative بس الله يقولنا اللي
+
+475
+00:34:43,430 --> 00:34:45,950
+هي الـsolomonas و الـenterobacteriasis كذا بفرق
+
+476
+00:34:45,950 --> 00:34:49,850
+بينهم و بفحص الأكسداز و فحص الجليكوز fermentation
+
+477
+00:34:50,520 --> 00:34:53,540
+بيندي لجرام نيجاتيف تيبلو كوكاى اللى هى ال موريكز
+
+478
+00:34:53,540 --> 00:34:57,380
+الكاتريليز و نيسيريا تنتهى ان اكسيداس positive و
+
+479
+00:34:57,380 --> 00:35:01,140
+كتالياز positive كيف افرق بينهم بيندي فحص ال
+
+480
+00:35:01,140 --> 00:35:05,060
+بتيوريات تست بتيوريات تست بيكشف ليه عن انزيم ال
+
+481
+00:35:05,060 --> 00:35:10,380
+بتيوريات استيرياز انزايم هذا الانزايمموجود في
+
+482
+00:35:10,380 --> 00:35:14,580
+الموريكزيلا كاتريلز و positive في الموريكزيلا
+
+483
+00:35:14,580 --> 00:35:17,560
+كاتريلز و negative في النيسيريا يبقى اللي بدنا
+
+484
+00:35:17,560 --> 00:35:20,900
+نعرفه ان انا عشان افرق الموريكزيلا عن النيسيريا في
+
+485
+00:35:20,900 --> 00:35:24,360
+عندى أنزيم او فحص ال biothiorate test بيكشف على
+
+486
+00:35:24,360 --> 00:35:27,700
+أنزيم ال biothiorate stress enzyme positive في
+
+487
+00:35:27,700 --> 00:35:32,210
+الموريكزيلا كاتريلز و negative في النيسيرياهذا ال
+
+488
+00:35:32,210 --> 00:35:35,530
+slide كملنا قلنا لو كان فيه اندي جرامستين ممكن
+
+489
+00:35:35,530 --> 00:35:38,070
+تكون جرام positive أو negative الجرام positive و
+
+490
+00:35:38,070 --> 00:35:42,270
+negative كل منها بنقسم لك كوكاي و بصلاي لو كانت
+
+491
+00:35:42,270 --> 00:35:45,790
+جرام positive بصلاي بعمل فحص ال catalase إذا طلعت
+
+492
+00:35:45,790 --> 00:35:47,850
+negative يبقى strepto إذا طلعت positive في
+
+493
+00:35:47,850 --> 00:35:51,770
+انديستافيل و كوكاي ماكرو كوكاي مايكرو كوكايكيف بدى
+
+494
+00:35:51,770 --> 00:35:56,230
+افرق بينهم بفحص الاكسداز فحص الاكسداز لو طلعت
+
+495
+00:35:56,230 --> 00:35:59,990
+اكسداز positive يبقى هي macro او micro لو طلعت
+
+496
+00:35:59,990 --> 00:36:03,390
+اكسداز negative يبقى هي Staphylococci طب كيف بدى
+
+497
+00:36:03,390 --> 00:36:06,670
+افرق Staphylococci species عن بعض بقول بفحص ال
+
+498
+00:36:06,670 --> 00:36:09,690
+coagulase لو coagulase positive يبقى هي
+
+499
+00:36:09,690 --> 00:36:12,910
+Staphylococcus aureus لو coagulase negative يبقى
+
+500
+00:36:12,910 --> 00:36:15,670
+هي Staphylococcus epidermidis او Staphylococcus
+
+501
+00:36:15,670 --> 00:36:20,070
+saprophyticبالنسبة لـ Ground Positive Bacilli بعمل
+
+502
+00:36:20,070 --> 00:36:22,710
+فحص الكيتالياز لو طلعت positive يبقى بيبقى باصلص
+
+503
+00:36:22,710 --> 00:36:26,570
+أو الستيريا مونو سايتوجين لو طلعت negative يبقى هي
+
+504
+00:36:26,570 --> 00:36:29,750
+Clostridium وكيف ده أفرق الباصلص عن الستيريا مونو
+
+505
+00:36:29,750 --> 00:36:32,630
+سايتوجين بالاسباريستان الباصلص sport farming
+
+506
+00:36:32,630 --> 00:36:37,550
+الاستيريا مونو سايتوجين non sport farmingالجرام
+
+507
+00:36:37,550 --> 00:36:41,550
+نيجاتيف ديبلوكوكاي كتلاز والأكسداز لإلهم positive
+
+508
+00:36:41,550 --> 00:36:44,230
+اللي هي الموريكزيلا والنيسيريا عشان أفرق بينهم
+
+509
+00:36:44,230 --> 00:36:47,490
+بعمل فحص الperturate استيراز ال positive
+
+510
+00:36:47,490 --> 00:36:50,770
+الموريكزيلا والنيجاتيف النيسيريا الجرام نيجاتيف
+
+511
+00:36:50,770 --> 00:36:56,810
+باسلايالجرام نيجاتيب بسال لايف بتضم الصدوموناس
+
+512
+00:36:56,810 --> 00:37:00,050
+الهيليكوباكترياسي وكمان في عندي الاسينيتوباكتر ال
+
+513
+00:37:00,050 --> 00:37:03,970
+Vibrio الهيليكوباكتر بقول عشان افرق بينهم بعمل فحص
+
+514
+00:37:03,970 --> 00:37:06,930
+الاكسيداز لو طلعت positive يبقى احتمال عندي لو
+
+515
+00:37:06,930 --> 00:37:11,270
+طلعت اكسيداز positive اما صدوموناس او Vibrio لو
+
+516
+00:37:11,270 --> 00:37:14,510
+طلعت اكسيداز نيجاتيب قلنا الهيليكوباكترياسي في
+
+517
+00:37:14,510 --> 00:37:18,070
+كمان الاسينيتوباكتر كيف بدي افرق الصدوموناس عن ال
+
+518
+00:37:18,070 --> 00:37:22,640
+Vibrio بقول بفحص الجليكوزلو طلعت negative يبقى هي
+
+519
+00:37:22,640 --> 00:37:25,560
+Sodomonas لو طلعت positive هي Vibrio أو
+
+520
+00:37:25,560 --> 00:37:29,580
+Helicobacter كيف أفرق ال Acetobacter و ال
+
+521
+00:37:29,580 --> 00:37:32,100
+Enterobacteriasi لو طلع ال Oxidase negative بقولي
+
+522
+00:37:32,100 --> 00:37:35,020
+برضه بفحص الجليكوز fermenter لو طلعت negative فهي
+
+523
+00:37:35,020 --> 00:37:38,000
+Acetobacter لو طلعت positive يبقى هي
+
+524
+00:37:38,000 --> 00:37:40,780
+Enterobacteriasi ال Enterobacteriasi قلنا عائلة
+
+525
+00:37:40,780 --> 00:37:43,620
+كبيرة ممكن انها تكون بتقسم لLactose fermenter و
+
+526
+00:37:43,620 --> 00:37:47,310
+Non-lactose fermenterلو طلعت Lactose Fermenter
+
+527
+00:37:47,310 --> 00:37:51,770
+بتنقسم لفيكل ونونفيكل الفيكل فيه عند الـ E.coli وE
+
+528
+00:37:51,770 --> 00:37:53,630
+.clepsula الـ non-fecal للـ Enterobacter
+
+529
+00:37:53,630 --> 00:37:57,510
+والستروبكتر كيف بفرق بينهم بفحص السيطرات E.coli
+
+530
+00:37:57,510 --> 00:38:00,250
+negative لكن الباقي positive أو فحص الـ motility
+
+531
+00:38:00,250 --> 00:38:03,450
+ال E.clepsula non-motile والباقي motile يبقى هذا
+
+532
+00:38:03,450 --> 00:38:08,790
+هو هيك تقريبا حاولنا أن نلخص الفحوصات عشان نتعرف
+
+533
+00:38:08,790 --> 00:38:14,590
+على البكتيرياهذه هي ال slide ال oxidase slide test
+
+534
+00:38:14,590 --> 00:38:17,930
+هاي ال slide بتكون مغمورة بال reagent بال
+
+535
+00:38:17,930 --> 00:38:22,290
+substrate لما أنا أخد البكتيريا أخدت البكتيريا و
+
+536
+00:38:22,290 --> 00:38:25,670
+حطيتها على هاي ال slide صار اللون أو ما تغير كان
+
+537
+00:38:25,670 --> 00:38:29,570
+colorless يبقى هي oxidase negative بكتيريا لكن لو
+
+538
+00:38:29,570 --> 00:38:32,970
+اتغير اللون للون purple يبقى هي oxidase positive
+
+539
+00:38:32,970 --> 00:38:38,340
+بكتيرياهنا في عندي filter paper انا باغمره
+
+540
+00:38:38,340 --> 00:38:42,860
+بالرياجانت وبعدين باخد البكتيريا اللى اتحول للون
+
+541
+00:38:42,860 --> 00:38:45,660
+بيربول يبقى اكسيداز positive لو ضاله لون colorless
+
+542
+00:38:45,660 --> 00:38:49,600
+يبقى اكسيداز negative هنا بشكل مباشر اجبت
+
+543
+00:38:49,600 --> 00:38:52,880
+الرياجانت وغمرته بالكولوني اذا اتحول لون الكولوني
+
+544
+00:38:52,880 --> 00:38:54,680
+للون بيربول يبقى positive
+
+545
+00:38:57,100 --> 00:39:02,100
+جبت slide حطيت عليها اللى هو ال filter بابر غمرت
+
+546
+00:39:02,100 --> 00:39:06,600
+ال reagent أنا غمرت ال reagent بيه ال filter حطيت
+
+547
+00:39:06,600 --> 00:39:09,740
+ال reagent على ال filter بابر بعدين جبت البكتيريا
+
+548
+00:39:10,150 --> 00:39:13,750
+وشفت إذا اتحول اللون للون violet يبقى هي positive
+
+549
+00:39:13,750 --> 00:39:18,510
+لكن لو ما اتغير يبقى هي negative يبقى عندي أكتر من
+
+550
+00:39:18,510 --> 00:39:21,890
+طريقة للفحص إما يكون ال reagent ال reagent بشكل
+
+551
+00:39:21,890 --> 00:39:24,990
+مباشر أديره على ال colony أو أجيب filter بابر أغمر
+
+552
+00:39:24,990 --> 00:39:28,670
+ال reagent أنا بعدين أحط البكتيريا ممكن يكون في
+
+553
+00:39:28,670 --> 00:39:33,340
+عندي اللي هو stripهي الـ Strip أو الـ Slide بيكون
+
+554
+00:39:33,340 --> 00:39:36,460
+مغمورة بالاصل بالـ reagent أنا فقط بضيف الـ Colony
+
+555
+00:39:36,460 --> 00:39:40,020
+ممكن يكون في عندي desk ال desk هذا برضه نفس الفكرة
+
+556
+00:39:40,020 --> 00:39:45,540
+بيكون مغمور بالـ reagent بمسك ال desk بنيدل وباخد
+
+557
+00:39:45,540 --> 00:39:49,400
+Colony من الطبق إذا اتحول اللون ال desk للون
+
+558
+00:39:49,400 --> 00:39:52,380
+violet يبقى هو positive يبقى باختلاف الطرق إذا
+
+559
+00:39:52,380 --> 00:39:55,240
+اتحول اللون للون violet أو dark purple يبقى هي
+
+560
+00:39:55,240 --> 00:39:59,860
+positive لكن لو كان colorless فهي negative هيك
+
+561
+00:39:59,860 --> 00:40:03,950
+بنكون خلصناالـ Citrate Utilization and Oxidase
+
+562
+00:40:03,950 --> 00:40:08,710
+Test الاتنين Indo Enzyme يعطيكم العافية جميعا
+
+563
+00:40:08,710 --> 00:40:11,350
+والسلام عليكم ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ek7K6HogOhs.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ek7K6HogOhs.srt
new file mode 100644
index 0000000000000000000000000000000000000000..1810bc6aa8015530d01904c28cfe0151ef7864eb
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ek7K6HogOhs.srt
@@ -0,0 +1,47 @@
+1
+00:00:21,230 --> 00:00:25,730
+السلام عليكم ورحمة الله وبركاته، نستكمل معكم سلسلة
+
+2
+00:00:25,730 --> 00:00:29,910
+Microbiology الجامعة الإسلامية، فيديو اليوم بعنوان
+
+3
+00:00:29,910 --> 00:00:31,070
+«جرام الثاني»
+
+4
+00:00:56,650 --> 00:00:59,870
+والآن لنتعرف على الأدوات التي تستخدم في خطوات العمل
+
+5
+00:00:59,870 --> 00:01:04,610
+أولاً لدينا الصبغة الأولية وهي Gram crystal violet
+
+6
+00:01:04,610 --> 00:01:08,010
+ثم
+
+7
+00:01:08,010 --> 00:01:14,670
+لدينا Gram iodine وهو المثبت ثم
+
+8
+00:01:14,670 --> 00:01:17,930
+لدينا Gram decolorizer وهو عبارة عن ميثانول بتركيز
+
+9
+00:01:17,930 --> 00:01:18,870
+95%
+
+10
+00:01:22,520 --> 00:01:25,060
+ولدينا الـ counter-stain وهي عبارة عن صبغة
+
+11
+00:01:25,060 --> 00:01:31,080
+المعاكسة الصفرانين, sterile loops, slides, normal
+
+12
+00:01:31,080 --> 00:01:34,120
+saline والبكتيريا، سلبية الجرام وموجبة الجرام
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ek7K6HogOhs_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ek7K6HogOhs_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..c264d1850bbd0c4744ff621fefa880a5c856f650
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ek7K6HogOhs_postprocess.srt
@@ -0,0 +1,48 @@
+1
+00:00:21,230 --> 00:00:25,730
+السلام عليكم ورحمة الله وبركاته، نستكمل معكم سلسلة
+
+2
+00:00:25,730 --> 00:00:29,910
+Microbiology الجامعة الإسلامية، فيديو اليوم بعنوان
+
+3
+00:00:29,910 --> 00:00:31,070
+«جرام الثاني»
+
+4
+00:00:56,650 --> 00:00:59,870
+والآن لنتعرف عن الأدوات التي تستخدم في خطوات العمل
+
+5
+00:00:59,870 --> 00:01:04,610
+أولاً لدينا الصبغة الأولية وهي gram crystal violet
+
+6
+00:01:04,610 --> 00:01:08,010
+ثم
+
+7
+00:01:08,010 --> 00:01:14,670
+لدينا gram iodine وهو المثبت ثم
+
+8
+00:01:14,670 --> 00:01:17,930
+لدينا gram decalorizer وهو عبارة عن ميثانو بتركيز
+
+9
+00:01:17,930 --> 00:01:18,870
+95%
+
+10
+00:01:22,520 --> 00:01:25,060
+وداينا ال counter-stain وهي عبارة عن أصابع
+
+11
+00:01:25,060 --> 00:01:31,080
+المعاكسة الصفرانين, sterile loops, slide, normal
+
+12
+00:01:31,080 --> 00:01:34,120
+saline والبكتيريا، سلبة جرامة وموجة متجرامة
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ek7K6HogOhs_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ek7K6HogOhs_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..c264d1850bbd0c4744ff621fefa880a5c856f650
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ek7K6HogOhs_raw.srt
@@ -0,0 +1,48 @@
+1
+00:00:21,230 --> 00:00:25,730
+السلام عليكم ورحمة الله وبركاته، نستكمل معكم سلسلة
+
+2
+00:00:25,730 --> 00:00:29,910
+Microbiology الجامعة الإسلامية، فيديو اليوم بعنوان
+
+3
+00:00:29,910 --> 00:00:31,070
+«جرام الثاني»
+
+4
+00:00:56,650 --> 00:00:59,870
+والآن لنتعرف عن الأدوات التي تستخدم في خطوات العمل
+
+5
+00:00:59,870 --> 00:01:04,610
+أولاً لدينا الصبغة الأولية وهي gram crystal violet
+
+6
+00:01:04,610 --> 00:01:08,010
+ثم
+
+7
+00:01:08,010 --> 00:01:14,670
+لدينا gram iodine وهو المثبت ثم
+
+8
+00:01:14,670 --> 00:01:17,930
+لدينا gram decalorizer وهو عبارة عن ميثانو بتركيز
+
+9
+00:01:17,930 --> 00:01:18,870
+95%
+
+10
+00:01:22,520 --> 00:01:25,060
+وداينا ال counter-stain وهي عبارة عن أصابع
+
+11
+00:01:25,060 --> 00:01:31,080
+المعاكسة الصفرانين, sterile loops, slide, normal
+
+12
+00:01:31,080 --> 00:01:34,120
+saline والبكتيريا، سلبة جرامة وموجة متجرامة
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/fZnjal05seA.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/fZnjal05seA.srt
new file mode 100644
index 0000000000000000000000000000000000000000..16c8bb83b75e38555aa5cabd5c85c38e6dd3d2a9
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/fZnjal05seA.srt
@@ -0,0 +1,682 @@
+1
+00:00:00,830 --> 00:00:03,890
+السلام عليكم اليوم هنحكي عن مساق  Practical
+
+2
+00:00:03,890 --> 00:00:08,230
+Essential Medical Microbiology في هذا اللقاء
+
+3
+00:00:08,230 --> 00:00:13,170
+هنتعرف على علم الـ Microbiology، تعريف هذا العلم
+
+4
+00:00:13,170 --> 00:00:17,790
+فروع علم الـ Microbiology والهدف من قسم الـ
+
+5
+00:00:17,790 --> 00:00:22,290
+Microbiology  في المستشفيات. هنتعرف على
+
+6
+00:00:22,290 --> 00:00:27,090
+المواضيع اللي هنتناولها في هذا المساق وتوزيعها
+
+7
+00:00:27,090 --> 00:00:34,660
+خلال الفصل. وآخر شيء هنحكي عن توزيع درجات المساق
+
+8
+00:00:34,660 --> 00:00:41,780
+بالنسبة لعلم الـ Microbiology، لو جزأنا كلمة الـ
+
+9
+00:00:41,780 --> 00:00:47,560
+Microbiology، طبعًا هو العلم it is the study of
+
+10
+00:00:47,560 --> 00:00:54,950
+حيوي، وmicro دقيق. يبقى هو علم الأحياء الدقيقة. الـ
+
+11
+00:00:54,950 --> 00:00:58,330
+Microbiology، لو أنا بدي أعرفه، it is the
+
+12
+00:00:58,330 --> 00:01:03,510
+study of microorganisms. هو علم دراسة الكائنات
+
+13
+00:01:03,510 --> 00:01:08,350
+الدقيقة. طب لو بدي أعرف الـ microorganism، بحكي it
+
+14
+00:01:08,350 --> 00:01:12,650
+is a small living organism that cannot be seen by the
+
+15
+00:01:12,650 --> 00:01:17,450
+naked eye. هو العلم الذي يدرس الكائنات الدقيقة
+
+16
+00:01:17,450 --> 00:01:23,110
+الحية التي لا ترى بالعين المجردة. علم الـ Microbiology
+
+17
+00:01:23,110 --> 00:01:30,370
+هو علم واسع طبعًا له أفرع، له أفرع مختلفة. الـ
+
+18
+00:01:30,370 --> 00:01:36,350
+Microbiology يضم عدة فروع: Medical Microbiology، و
+
+19
+00:01:36,350 --> 00:01:41,150
+Soil Microbiology، وFood Microbiology. طبعًا أنتم
+
+20
+00:01:41,150 --> 00:01:45,890
+في عينكم مساق الـ Food Microbiology اللي طبعًا
+
+21
+00:01:45,890 --> 00:01:50,550
+تطبيقاته في مجال الأبحاث وفي مجال رقابة وجودة
+
+22
+00:01:50,550 --> 00:01:55,140
+الأغذية. فيه كمان Water and Sewage Microbiology،
+
+23
+00:01:55,140 --> 00:02:00,360
+Industrial Microbiology، وMicrobial Genetics. يبقى
+
+24
+00:02:00,360 --> 00:02:05,900
+علم الـ Microbiology علم واسع بيضم عدة أقسام تحته.
+
+25
+00:02:05,900 --> 00:02:11,820
+طبعًا إحنا كأخصائي تحاليل طبية، هندرس اللي هو الـ
+
+26
+00:02:11,820 --> 00:02:15,000
+Medical Microbiology، اللي إيه علاقته في اللي هو
+
+27
+00:02:15,000 --> 00:02:20,820
+الجانب الطبي. بالنسبة لـ Medical Microbiology، طبعًا
+
+28
+00:02:20,820 --> 00:02:26,180
+هو علم كبير وواسع، عشان هيك هندرسه في عدة مساقات
+
+29
+00:02:26,180 --> 00:02:31,700
+خلال سنوات الدراسة. الـ Medical Microbiology بضم
+
+30
+00:02:31,700 --> 00:02:36,620
+طبعًا أيضًا عدة فروع. أول فرع اللي هو الـ Bacteriology،
+
+31
+00:02:36,620 --> 00:02:41,340
+هو علم دراسة البكتيريا. في هذا المساق هنركز على
+
+32
+00:02:41,340 --> 00:02:45,840
+دراسة البكتيريا. هنتعامل مع البكتيريا. في عينينا الـ
+
+33
+00:02:45,840 --> 00:02:51,040
+Virology. طبعًا الـ Virology هو علم دراسة الفيروسات.
+
+34
+00:02:51,040 --> 00:02:56,760
+أخذتوها في سنة ثانية. الـ Mycology وهو علم الفطريات، و
+
+35
+00:02:56,760 --> 00:03:00,100
+الـ Protozoology هو علم الأوليات. طبعًا هدول
+
+36
+00:03:00,100 --> 00:03:06,040
+العلمين هتاخدوهم في مستوى رابع. بالنسبة لـ
+
+37
+00:03:06,040 --> 00:03:10,340
+Immunology، وهو علم المناعة. علم المناعة كان قديمًا
+
+38
+00:03:10,340 --> 00:03:15,540
+هو فرع من فروع الـ Medical Microbiology، لكن مع كبر
+
+39
+00:03:15,540 --> 00:03:19,100
+حجم المعلومات في علم المناعة، تم فصله عن الـ
+
+40
+00:03:19,100 --> 00:03:25,000
+Microbiology. أنا بتكلم عن microorganism، جسم غريب،
+
+41
+00:03:25,000 --> 00:03:30,360
+طبعًا antigen، دخل جسم الإنسان، أكيد هيلاقي رد فعل من
+
+42
+00:03:30,360 --> 00:03:35,340
+جهاز المناعة، ويكون له أجسام مضادة. بالتالي لا يمكن
+
+43
+00:03:35,340 --> 00:03:41,080
+فصل الـ Microbiology عن الـ Immunology في الدراسة.
+
+44
+00:03:43,240 --> 00:03:47,740
+طبعًا هيك عرفنا شو تعريف علم الـ Microbiology، شو
+
+45
+00:03:47,740 --> 00:03:51,800
+أقسام علم الـ Microbiology، وعرفنا فروع الـ Medical
+
+46
+00:03:51,800 --> 00:03:56,860
+Microbiology. الهدف من قسم الـ Microbiology في
+
+47
+00:03:56,860 --> 00:04:00,940
+المستشفيات، يهدف إلى عزل البكتيريا من العينات
+
+48
+00:04:00,940 --> 00:04:05,880
+المرضية وتشخيصها عبر فحوصات مختلفة. طيب إحنا
+
+49
+00:04:05,880 --> 00:04:10,840
+هنتعامل مع عينات مرضية، يعني احتمال كبير يكون فيها
+
+50
+00:04:10,840 --> 00:04:15,280
+pathogenic microorganisms. بالتالي إجراءات السلامة
+
+51
+00:04:15,280 --> 00:04:19,500
+والوقاية ضرورية، إننا نتبعها في معمل الـ
+
+52
+00:04:19,500 --> 00:04:23,880
+Microbiology. عشان هيك في أول أسبوع هيكون في عندنا
+
+53
+00:04:23,880 --> 00:04:28,460
+مقدمة. المقدمة هتكون عبارة عن Safety Rules، قواعد
+
+54
+00:04:28,460 --> 00:04:33,240
+السلامة داخل معمل الـ Microbiology. وهنتكلم أيضًا
+
+55
+00:04:33,240 --> 00:04:38,380
+عن الأدوات في معمل الـ Microbiology: Instruments in
+
+56
+00:04:38,380 --> 00:04:42,620
+Microbiology Lab. هنتعرف على الأدوات الخاصة بالمعمل.
+
+57
+00:04:43,410 --> 00:04:48,170
+الأسبوع الثاني، هنتكلم عن الـ Microscope. بالنسبة للـ
+
+58
+00:04:48,170 --> 00:04:52,770
+Microscope، إحنا نتعامل مع microorganisms، مع
+
+59
+00:04:52,770 --> 00:04:58,510
+كائنات أصغر من العين المجردة. بالتالي أكيد أنا هحتاج
+
+60
+00:04:58,510 --> 00:05:02,830
+الـ Microscope حتى إن أنا أكبرها وأشوفها بشكل واضح.
+
+61
+00:05:03,310 --> 00:05:06,750
+في هذا المساق، هنتعرف على الميكروسكوب، أجزاء
+
+62
+00:05:06,750 --> 00:05:12,530
+الميكروسكوب، وظيفة كل جزء. هنتعرف على كيف أننا
+
+63
+00:05:12,530 --> 00:05:16,610
+نستخدم الميكروسكوب، وكيف أقدر أجيب الـ field بشكل
+
+64
+00:05:16,610 --> 00:05:22,010
+صحيح. بالنسبة للأسبوع الثالث والأسبوع الرابع،
+
+65
+00:05:22,010 --> 00:05:26,430
+والأسبوع الخامس، كلها عبارة عنها هتكون عن صبغات.
+
+66
+00:05:26,430 --> 00:05:32,760
+في معمل السوائل اللي أخذتوها فيه الـ Urine
+
+67
+00:05:32,760 --> 00:05:38,620
+Analysis، كان في عندي Microscopic Examination، وفي
+
+68
+00:05:38,620 --> 00:05:43,040
+عندي من ضمن الـ Microscopic Examination، في عندي فرع
+
+69
+00:05:43,040 --> 00:05:50,280
+اللي هو البكتيريا. لو أنا طبعًا جبت عينة مريض بالـ
+
+70
+00:05:50,280 --> 00:05:54,120
+UTI، اللي هو الـ Urinary Tract Infection، التهاب
+
+71
+00:05:54,120 --> 00:05:58,730
+المسالك البولية. هذا المريض، لو جبت عينة يورين وعملت
+
+72
+00:05:58,730 --> 00:06:02,050
+لها Centrifugation، وأخذت طبعًا، تخلصت من الـ
+
+73
+00:06:02,050 --> 00:06:05,730
+supernatant، وأخذت الـ sediment، وحطيته على slide، و
+
+74
+00:06:05,730 --> 00:06:10,490
+cover slip، وتحت الميكروسكوب، شيء أكيد هشوف تحت
+
+75
+00:06:10,490 --> 00:06:14,870
+الميكروسكوب في عينة اليورين، هشوف بكتيريا. هذه
+
+76
+00:06:14,870 --> 00:06:20,070
+البكتيريا، هشوفها حجمها صغير، وهشوفها
+
+77
+00:06:20,070 --> 00:06:25,240
+transparent، شفافة. طبعًا البكتيريا transparent، شفافة،
+
+78
+00:06:25,240 --> 00:06:29,100
+مش هقدر أشوفها بشكل واضح كثير، هتحتاج لقوة ملاحظة
+
+79
+00:06:29,100 --> 00:06:33,780
+عشان هيك بلجأ للصبغات، للصبغات، حتى إن أنا أقدر
+
+80
+00:06:33,780 --> 00:06:38,320
+أشوفها بشكل واضح. في عندي أنا أنواع كثيرة من
+
+81
+00:06:38,320 --> 00:06:42,800
+الصبغات وتصنيفات. أول شيء إن الـ Simple Stain، الـ
+
+82
+00:06:42,800 --> 00:06:47,770
+Simple Stain، بشكل مختصر، هنحكي، الهدف منها
+
+83
+00:06:47,770 --> 00:06:51,950
+اللي هو إن أنا أشوف الشكل، والarrangement، شكل و
+
+84
+00:06:51,950 --> 00:06:56,150
+ترتيب الخلايا، الخلية البكتيرية. بالنسبة
+
+85
+00:06:56,150 --> 00:06:59,930
+لـ Gram Stain، Gram Stain، يمكن سمعتوا فيها، بتقسم
+
+86
+00:06:59,930 --> 00:07:05,160
+البكتيريا لمجموعتين: موجبة جرام وسالبة جرام. الـ Acid
+
+87
+00:07:05,160 --> 00:07:09,940
+Fast Stain هي خاصة ببكتيريا السل الرئوي، بكتيريا
+
+88
+00:07:09,940 --> 00:07:12,420
+السل الرئوي اللي هي الـ Mycobacterium
+
+89
+00:07:12,420 --> 00:07:17,380
+tuberculosis. هذه البكتيريا ما بقدر إن أنا أصبغها
+
+90
+00:07:17,380 --> 00:07:24,860
+بالـ Gram Stain، تمام؟ فبالتالي، لجأنا لـ Acid Fast Stain
+
+91
+00:07:24,860 --> 00:07:31,200
+لصبغها. طبعًا هنعرف إيش السبب في عدم قدرتنا
+
+92
+00:07:31,200 --> 00:07:36,270
+لصبغها بالـ Gram Stain، في خاصية في هذه البكتيريا
+
+93
+00:07:36,270 --> 00:07:41,210
+طبعًا بتخليها ما نقدر نُصبغها بالـ Gram Stain.
+
+94
+00:07:41,210 --> 00:07:46,130
+بالأسبوع الخامس، فيه إننا هنكمل فيه موضوع الصبغات.
+
+95
+00:07:46,130 --> 00:07:51,230
+فيه إن أنا تراكيب خاصة في الخلية البكتيرية. في
+
+96
+00:07:51,230 --> 00:07:58,360
+هذا المساق، هنصبغ Special Stain، الصبغات الخاصة، هتصبغ
+
+97
+00:07:58,360 --> 00:08:02,260
+تراكيب خاصة داخل الخلية البكتيرية. في عندي الـ
+
+98
+00:08:02,260 --> 00:08:06,280
+Spore Stain، اللي هي الأبواغ، حتى إن أنا أعرف هل
+
+99
+00:08:06,280 --> 00:08:10,680
+البكتيريا قادرة على إنتاج الأبواغ أو لا، بستخدم الـ
+
+100
+00:08:10,680 --> 00:08:14,360
+Spore Stain. الـ Negative Stain، اللي هي اسم آخر
+
+101
+00:08:14,360 --> 00:08:16,740
+اللي هي الـ Capsule Stain، حتى إن أنا أعرف
+
+102
+00:08:16,740 --> 00:08:20,760
+البكتيريا، هل تمتلك Capsule (حافظة) أو لا، بصبغها بـ
+
+103
+00:08:20,760 --> 00:08:24,620
+Negative Stain. Bacterial Motility، عشان أعرف
+
+104
+00:08:24,620 --> 00:08:29,640
+البكتيريا هل هي motile أو non-motile، بستخدم طبعًا
+
+105
+00:08:29,640 --> 00:08:35,560
+اللي هي Flagella Stain. الفلاجلة يعتبر هو طبعًا
+
+106
+00:08:35,560 --> 00:08:39,100
+الجزء المسؤول عن الحركة، اللي هو الصوت. فعشان أعرف
+
+107
+00:08:39,100 --> 00:08:44,480
+هل هي motile أو non-motile، بصبغ الصوت. طبعًا هذا
+
+108
+00:08:44,480 --> 00:08:48,080
+بالنسبة للأسبوع الثالث والرابع والخامس، كلها هتكون
+
+109
+00:08:48,080 --> 00:08:53,660
+عبارة عن صبغات. هنستخدم أكيد الميكروسكوب، فبالتالي
+
+110
+00:08:53,660 --> 00:08:57,320
+لازم نكون طالعين من معمل الميكروسكوب عارفين نجيب
+
+111
+00:08:57,320 --> 00:09:01,480
+الـ field، لأن كل المعامل هتعتمد على الميكروسكوب.
+
+112
+00:09:03,340 --> 00:09:07,360
+في الأسبوع السادس، هنبدأ نحكي عن الـ Isolation، عزل
+
+113
+00:09:07,360 --> 00:09:11,380
+البكتيريا. طيب أنا ليش بدي أعزل بكتيريا؟ إحنا قلنا
+
+114
+00:09:11,380 --> 00:09:15,400
+هدفنا في قسم الـ Microbiology إننا نعزل بكتيريا من
+
+115
+00:09:15,400 --> 00:09:20,500
+عينات مرضية ونشخصها عبر فحوصات مختلفة. إيش يعني
+
+116
+00:09:20,500 --> 00:09:24,080
+نشخصها؟ يعني بدي أعطي اسم للبكتيريا المسببة
+
+117
+00:09:24,080 --> 00:09:28,800
+للالتهاب، بدي أعرف هويتها، اللي على أساسه الدكتور
+
+118
+00:09:28,800 --> 00:09:33,180
+هيوصف العلاج، اللي هو الـ antibiotic المناسب للمريض.
+
+119
+00:09:33,730 --> 00:09:37,830
+هلق أنا في أول معامل لما أخدت العينة وشفتها تحت
+
+120
+00:09:37,830 --> 00:09:42,150
+الـ Microscope وصبغتها، فقط عرفت إنه أنا فيها أي
+
+121
+00:09:42,150 --> 00:09:45,970
+infection أو لا، في بكتيريا في العينة أو لا. عرفت
+
+122
+00:09:45,970 --> 00:09:50,290
+معلومات قليلة، إنه هاي مثلًا من أي مجموعة، ما قدرت
+
+123
+00:09:50,290 --> 00:09:55,470
+أوْصل لاسم البكتيريا. عشان هيك لازم أنا أعزلها من
+
+124
+00:09:55,470 --> 00:10:00,930
+العينة، عشان الصورة تكون أوضح. بدنا نشبه البكتيريا
+
+125
+00:10:00,930 --> 00:10:05,970
+بالجنين والعينة بالأم. هلق الـ baby طول ما هو، طول
+
+126
+00:10:05,970 --> 00:10:11,390
+ما هو ببطن أمه، فقط بقدر أعرف إنه هل الأم حامل أو لا،
+
+127
+00:10:11,390 --> 00:10:16,010
+هل فيه baby أو لا، ممكن أعرف مثلًا جنسه، جنس الجنين،
+
+128
+00:10:16,340 --> 00:10:20,840
+لكن تفاصيل أكثر، لون بشرته، لون عينيه، ما بقدر، لازم
+
+129
+00:10:20,840 --> 00:10:26,020
+يولد الطفل وأشوفه حتى أقدر أعرف هذه التفاصيل. كذلك
+
+130
+00:10:26,020 --> 00:10:31,040
+البكتيريا، لازم أعزلها من العينة وأتعامل معها عشان
+
+131
+00:10:31,040 --> 00:10:36,520
+أعرف هذه التفاصيل وأقدر أشخصها. بالنسبة للجنين،
+
+132
+00:10:36,520 --> 00:10:42,640
+الجنين، حتى يولد ويخرج للحياة، لازم ينمو ويكبر، و
+
+133
+00:10:42,640 --> 00:10:47,840
+بالنهاية يتكون جسم إنسان كامل. برضه الجنين بيحتاج
+
+134
+00:10:47,840 --> 00:10:52,280
+حتى ينمو ويكبر لغذاء. هذا الغذاء بياخذه من طبعًا
+
+135
+00:10:52,280 --> 00:10:57,080
+أمه، ولازم يكون في بيئة مناسبة لنموه وبيئة مناسبة
+
+136
+00:10:57,080 --> 00:11:01,960
+لحمايته. طبعًا هذه البيئة هي رحم أمه. هنتعامل مع
+
+137
+00:11:01,960 --> 00:11:06,920
+البكتيريا بنفس تعاملنا مع الجنين حتى نقدر نعزلها.
+
+138
+00:11:07,270 --> 00:11:12,210
+أول شيء، البكتيريا برضه لازم تنمو وتنقسم. طبعًا
+
+139
+00:11:12,210 --> 00:11:16,550
+البكتيريا تعتبر unicellular، يعني وحيدة الخلية،
+
+140
+00:11:16,550 --> 00:11:22,170
+بتنقسم بواسطة الانشطار الثنائي، وبتنمو بشكل أُسّي، و
+
+141
+00:11:22,170 --> 00:11:26,250
+بتكون ملايين من الخلايا. هذه الخلايا بتتجمع مع
+
+142
+00:11:26,250 --> 00:11:30,630
+بعضها البعض، وبتكون ما يعرف بالـ Colony. الـ Colony هو
+
+143
+00:11:30,630 --> 00:11:36,610
+المستعمرة. هذا مصطلح الـ Colony. الـ Colony هي عبارة عن
+
+144
+00:11:36,610 --> 00:11:43,750
+مستعمرة. هذا طبعًا شكلها. هذه بنسميها Colony، مستعمرة. هذا
+
+145
+00:11:43,750 --> 00:11:48,810
+الشكل يعتبر Colony، تجمع من ملايين من الخلايا
+
+146
+00:11:48,810 --> 00:11:52,810
+البكتيريا، تجمعت حوالي بعضها، وكونت ما يعرف بالـ
+
+147
+00:11:52,810 --> 00:11:59,500
+Colony. طبعًا البكتيريا أنا ما بقدر أشوفها في الواقع
+
+148
+00:11:59,500 --> 00:12:03,380
+إلا على شكل Colony، زي الجنين ما بقدر أشوفه إلا على
+
+149
+00:12:03,380 --> 00:12:07,760
+شكل جسم إنسان كامل. ثاني شيء، البكتيريا، قلنا إنه
+
+150
+00:12:07,760 --> 00:12:13,540
+حتى تنمو بتحتاج لغذاء. الغذاء للبكتيريا بنسميه Media.
+
+151
+00:12:15,460 --> 00:12:20,660
+Media هو الوسط الغذائي للبكتيريا. ففي هذا المساق هنتعرف
+
+152
+00:12:20,660 --> 00:12:24,940
+كيف هنحضر الـ Media، كيف هنحضر الوسط الغذائي
+
+153
+00:12:24,940 --> 00:12:29,520
+للبكتيريا. طبعًا هذا طبق بتري. بالنسبة لـ Media، هي
+
+154
+00:12:29,520 --> 00:12:41,180
+المادة الحمراء. تمام؟ هي المادة الحمراء. لحظة.
+
+155
+00:12:44,740 --> 00:12:48,840
+هذه المادة الحمراء تعتبر هي الـ Media. هنتعرف كيف
+
+156
+00:12:48,840 --> 00:12:53,420
+نحضرها. طبعًا بنصبها في طبق البتري ديش. بعد كده نجيب
+
+157
+00:12:53,420 --> 00:12:56,660
+العينة اللي أنا شاكك فيها في أي Infection، التهاب،
+
+158
+00:12:56,660 --> 00:13:01,760
+بزرع هذه العينة، وبحطها في جهاز Incubator. بعد 24
+
+159
+00:13:01,760 --> 00:13:06,980
+ساعة بشوف نمو البكتيريا على شكل مستعمرة، تمام؟ طيب
+
+160
+00:13:07,180 --> 00:13:11,840
+بالنسبة للجنين، قلنا الجنين طبعًا حاضنته، الحاضنة
+
+161
+00:13:11,840 --> 00:13:15,760
+الأساسية هي رحم الأم. طول فترة التسعة شهور بيكون في
+
+162
+00:13:15,760 --> 00:13:20,200
+رحم الأم. طيب البكتيريا حتى تنمو وتتكاثر بدها اللي
+
+163
+00:13:20,200 --> 00:13:24,200
+هي حاضنة. حاضنة البكتيريا اسمها Incubator. هذا
+
+164
+00:13:24,200 --> 00:13:28,560
+الجهاز هنتعرف عليه في أول معمل من معامل الـ
+
+165
+00:13:28,560 --> 00:13:33,220
+Microbiology. هذه الـ Incubator هي عبارة عن جهاز
+
+166
+00:13:33,220 --> 00:13:37,740
+بوفر الظروف المناسبة لنمو البكتيريا، زي ما رحم الأم
+
+167
+00:13:37,740 --> 00:13:41,400
+بوفر الظروف المناسبة لنمو الـ baby، والـ Incubator
+
+168
+00:13:41,400 --> 00:13:47,990
+بوفر الظروف المناسبة لنمو الـ baby. البكتيريا بالنسبة
+
+169
+00:13:47,990 --> 00:13:51,810
+للأسبوع السادس، قلنا Media Preparation and
+
+170
+00:13:51,810 --> 00:13:55,470
+Sterilization. الـ Sterilization معناها تعقيم، to
+
+171
+00:13:55,470 --> 00:14:00,210
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/fZnjal05seA_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/fZnjal05seA_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..2175748745a2f6231bf6adc03069facbe7be7916
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/fZnjal05seA_raw.json
@@ -0,0 +1 @@
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{"start": 488.02, "end": 488.68, "word": " الأبواغ", "probability": 0.9781494140625}, {"start": 488.68, "end": 489.0, "word": " أو", "probability": 0.89794921875}, {"start": 489.0, "end": 489.38, "word": " لأ", "probability": 0.990478515625}, {"start": 489.38, "end": 490.54, "word": " بستخدم", "probability": 0.95654296875}, {"start": 490.54, "end": 490.68, "word": " ال", "probability": 0.822265625}, {"start": 490.68, "end": 491.14, "word": " Spore", "probability": 0.740966796875}, {"start": 491.14, "end": 491.94, "word": " Stain", "probability": 0.9755859375}, {"start": 491.94, "end": 492.42, "word": " الـ", "probability": 0.572509765625}, {"start": 492.42, "end": 492.66, "word": " Negative", "probability": 0.89013671875}, {"start": 492.66, "end": 493.34, "word": " Stain", "probability": 0.96435546875}, {"start": 493.34, "end": 493.62, "word": " اللى", "probability": 0.82080078125}, {"start": 493.62, "end": 493.72, "word": " هى", "probability": 0.730712890625}, {"start": 493.72, "end": 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{"start": 725.16, "end": 725.96, "word": " تاني", "probability": 0.8390299479166666}, {"start": 725.96, "end": 726.26, "word": " الشيء", "probability": 0.7355143229166666}, {"start": 726.26, "end": 726.84, "word": " البكتيريا", "probability": 0.95615234375}, {"start": 726.84, "end": 727.32, "word": " قلنا", "probability": 0.8260091145833334}, {"start": 727.32, "end": 727.76, "word": " إنه", "probability": 0.524658203125}, {"start": 727.76, "end": 728.06, "word": " حتى", "probability": 0.90185546875}, {"start": 728.06, "end": 728.48, "word": " تنمو", "probability": 0.934326171875}, {"start": 728.48, "end": 729.14, "word": " بتحتاج", "probability": 0.9329427083333334}, {"start": 729.14, "end": 730.02, "word": " لغذاء", "probability": 0.94775390625}, {"start": 730.02, "end": 731.0, "word": " الغذاء", "probability": 0.8931884765625}, {"start": 731.0, "end": 732.12, "word": " البكتيريا", "probability": 0.9875}, {"start": 732.12, "end": 732.94, "word": " منسمي", "probability": 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"probability": 0.68408203125}, {"start": 748.82, "end": 749.14, "word": " media", "probability": 0.470703125}, {"start": 749.14, "end": 749.52, "word": " هي", "probability": 0.352294921875}, {"start": 749.52, "end": 750.8, "word": " المادة", "probability": 0.947265625}, {"start": 750.8, "end": 752.18, "word": " الحمراء", "probability": 0.6764322916666666}, {"start": 752.18, "end": 752.86, "word": " تمام", "probability": 0.857177734375}, {"start": 752.86, "end": 753.7, "word": " هي", "probability": 0.7021484375}, {"start": 753.7, "end": 754.34, "word": " المادة", "probability": 0.9930013020833334}, {"start": 754.34, "end": 756.3, "word": " الحمراء", "probability": 0.9796549479166666}, {"start": 756.3, "end": 761.18, "word": " لحظة", "probability": 0.903564453125}], "temperature": 1.0}, {"id": 31, "seek": 78698, "start": 764.74, "end": 786.98, "text": "هذه المادة الحمرة تعتبر هي ال media هنتعرف كيف نحضرها طبعا نصبها في طبق البتري داش بعد كده نجيب العينة اللي أنا شاكه في اندي infection الديهاب بزرع هذه العينة و بحطها في جهاز incubator بعد 24 ساعة بشوف نمو البكتيريا على شكل مستعمرة تمام؟ طيب", "tokens": [3224, 24192, 9673, 18513, 3660, 21542, 2304, 25720, 6055, 34268, 26890, 39896, 2423, 3021, 8032, 29399, 3615, 28480, 9122, 33911, 8717, 5016, 11242, 2288, 11296, 23032, 3555, 3615, 995, 8717, 9381, 3555, 11296, 8978, 23032, 3555, 4587, 29739, 2655, 16572, 11778, 33599, 39182, 9122, 3215, 3224, 8717, 7435, 1829, 3555, 18863, 9957, 3660, 13672, 1829, 41850, 13412, 995, 4117, 3224, 8978, 16472, 16254, 11764, 2423, 16254, 11296, 3555, 4724, 11622, 2288, 3615, 29538, 18863, 9957, 3660, 4032, 4724, 5016, 9566, 11296, 8978, 10874, 3224, 31377, 33345, 1639, 39182, 4022, 8608, 995, 27884, 4724, 8592, 38688, 8717, 2304, 2407, 29739, 4117, 2655, 13546, 25528, 15844, 13412, 28820, 3714, 14851, 25957, 25720, 46811, 10943, 22807, 23032, 1829, 3555], "avg_logprob": -0.22288995471775022, "compression_ratio": 1.6816326530612244, "no_speech_prob": 3.719329833984375e-05, "words": 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1014.31, "word": " من", "probability": 0.953125}, {"start": 1014.31, "end": 1014.51, "word": " حد", "probability": 0.969970703125}, {"start": 1014.51, "end": 1014.59, "word": " ما", "probability": 0.9677734375}, {"start": 1014.59, "end": 1014.95, "word": " أشوف", "probability": 0.9791666666666666}, {"start": 1014.95, "end": 1015.35, "word": " pinpoint", "probability": 0.343994140625}, {"start": 1015.35, "end": 1016.03, "word": " على", "probability": 0.79248046875}, {"start": 1016.03, "end": 1017.09, "word": " الطبق", "probability": 0.9794921875}, {"start": 1017.09, "end": 1018.09, "word": " احتمالي", "probability": 0.898828125}, {"start": 1018.09, "end": 1018.27, "word": " كبير", "probability": 0.984375}, {"start": 1018.27, "end": 1018.61, "word": " بحكي", "probability": 0.898681640625}, {"start": 1018.61, "end": 1018.85, "word": " انه", "probability": 0.532958984375}, {"start": 1018.85, "end": 1019.05, "word": " high", "probability": 0.67529296875}, {"start": 1019.05, "end": 1020.05, "word": " streptolacin", "probability": 0.630224609375}, {"start": 1020.05, "end": 1020.57, "word": " لازم", "probability": 0.8961588541666666}, {"start": 1020.57, "end": 1021.39, "word": " نأكد", "probability": 0.8636474609375}, {"start": 1021.39, "end": 1022.07, "word": " ال", "probability": 0.44677734375}, {"start": 1022.07, "end": 1022.39, "word": " ..", "probability": 0.21533203125}, {"start": 1022.39, "end": 1023.15, "word": " طبعا", "probability": 0.9830322265625}, {"start": 1023.15, "end": 1023.63, "word": " نأكد", "probability": 0.9197998046875}, {"start": 1023.63, "end": 1023.85, "word": " اللي", "probability": 0.938232421875}, {"start": 1023.85, "end": 1024.07, "word": " هو", "probability": 0.99072265625}, {"start": 1024.07, "end": 1024.89, "word": " التشخيص", "probability": 0.9857421875}, {"start": 1024.89, "end": 1025.95, "word": " بفكوثات", "probability": 0.7206217447916666}, {"start": 1025.95, "end": 1026.75, "word": " كيميائية", "probability": 0.96708984375}, {"start": 1026.75, "end": 1027.81, "word": " هذا", "probability": 0.75244140625}, {"start": 1027.81, "end": 1029.25, "word": " بالنسبة", "probability": 0.9896240234375}, {"start": 1029.25, "end": 1031.53, "word": " لأسبوع", "probability": 0.8297119140625}, {"start": 1031.53, "end": 1032.23, "word": " السادس", "probability": 0.94970703125}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 1034.727625, "duration_after_vad": 1009.967500000001}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/fZnjal05seA_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/fZnjal05seA_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..f71d9c95bcd3102dbf298477b0301a87fff6a09a
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/fZnjal05seA_raw.srt
@@ -0,0 +1,848 @@
+1
+00:00:00,830 --> 00:00:03,890
+السلام عليكم اليوم هنحكي عن مساق ل practical
+
+2
+00:00:03,890 --> 00:00:08,230
+essential medical microbiology في هذا اللقاء
+
+3
+00:00:08,230 --> 00:00:13,170
+هنتعرف على علم ال microbiology تعريف هذا العلم
+
+4
+00:00:13,170 --> 00:00:17,790
+فروع علم ال microbiology و الهدف من قسم ال
+
+5
+00:00:17,790 --> 00:00:22,290
+microbiology ال biology في المستشفيات هنتعرف على
+
+6
+00:00:22,290 --> 00:00:27,090
+المواضيع اللي هنتناولها في هذا المساق و توزيها
+
+7
+00:00:27,090 --> 00:00:34,660
+خلال الفصلو آخر شئ هنحكي عن توزيع درجات المساق
+
+8
+00:00:34,660 --> 00:00:41,780
+بالنسبة لعلم ال microbiology لو جزأنا كلمة ال
+
+9
+00:00:41,780 --> 00:00:47,560
+microbiology طبعا هو العلم it is the study بيو
+
+10
+00:00:47,560 --> 00:00:54,950
+حيوي و micro دقيق يبقى هو علم الأحياء الدقيقةالـ
+
+11
+00:00:54,950 --> 00:00:58,330
+Microbiology لو أنا إجابة بدي أعرفه it is the
+
+12
+00:00:58,330 --> 00:01:03,510
+study of microorganism هو علم دراسة الكائنات
+
+13
+00:01:03,510 --> 00:01:08,350
+الدقيقة طب لو بدي أعرف ال micro organism بحكي it
+
+14
+00:01:08,350 --> 00:01:12,650
+is a small living organism that cannot be seen by
+
+15
+00:01:12,650 --> 00:01:17,450
+naked eye هو العلم الذي يدرس الكائنات الدقيقة
+
+16
+00:01:17,450 --> 00:01:23,110
+الحية التي لا ترى بالعين المجردعلم ال microbiology
+
+17
+00:01:23,110 --> 00:01:30,370
+هو علم واسع طبعا له أفرع له أفرع مختلفة ال
+
+18
+00:01:30,370 --> 00:01:36,350
+microbiology يضم عدة فروع medical microbiology و
+
+19
+00:01:36,350 --> 00:01:41,150
+soil microbiology و food microbiology طبعا انتوا
+
+20
+00:01:41,150 --> 00:01:45,890
+في عينكم أساق ال food microbiology اللي طبعا
+
+21
+00:01:45,890 --> 00:01:50,550
+تطبيقات في مجال الأبحاث و في مجال رقابة وجودة
+
+22
+00:01:50,550 --> 00:01:55,140
+الأغذيةفيه إنه كمان Water and Sewage Microbiology
+
+23
+00:01:55,140 --> 00:02:00,360
+Industrial Microbiology وMicrobial Genetic يبقى
+
+24
+00:02:00,360 --> 00:02:05,900
+علم الـ Microbiology علم واسع بيضم عدة أقسام تحته
+
+25
+00:02:05,900 --> 00:02:11,820
+طبعا إحنا كأخصائي تحاليل طبية هندرس اللي هو الـ
+
+26
+00:02:11,820 --> 00:02:15,000
+Medical Microbiology اللي إيه علاقة في اللي هو
+
+27
+00:02:15,000 --> 00:02:20,820
+الجانب الطبيبالنسبة لـ Medical Microbiology طبعا
+
+28
+00:02:20,820 --> 00:02:26,180
+هو علم كبير وواسع عشان هيك هندرسه في عدة مصاقات
+
+29
+00:02:26,180 --> 00:02:31,700
+خلال سنوات الدراسة ال medical microbiology بضم
+
+30
+00:02:31,700 --> 00:02:36,620
+طبعا أيضا عدة فروع أول فرع اللي هو ال bacteriology
+
+31
+00:02:36,620 --> 00:02:41,340
+هو علم دراسة البكتيريا في هذا المصاق هنركز على
+
+32
+00:02:41,340 --> 00:02:45,840
+دراسة البكتيريا هنتعامل مع البكتيريافي عيننا الـ
+
+33
+00:02:45,840 --> 00:02:51,040
+Virology طبعا الـ Virology هو علم دراسة الفيروسات
+
+34
+00:02:51,040 --> 00:02:56,760
+أخدته في سنة تانية الـ Mycology وهو علم الفطريات و
+
+35
+00:02:56,760 --> 00:03:00,100
+الـ Proto Zoology هو علم الأوليات طبعا هدول
+
+36
+00:03:00,100 --> 00:03:06,040
+العلمين هتاخدوهم في مستوى رابع بالنسبة لـ
+
+37
+00:03:06,040 --> 00:03:10,340
+Immunology وهو علم المناعة علم المناعة كان قديما
+
+38
+00:03:10,340 --> 00:03:15,540
+هو فرع من فروع ال medical microbiologyلكن مع كبر
+
+39
+00:03:15,540 --> 00:03:19,100
+حجم المعلومات في هلم المناعة تم فصله عن ال
+
+40
+00:03:19,100 --> 00:03:25,000
+microbiology أنا بتكلم عن micro organism جسم غريب
+
+41
+00:03:25,000 --> 00:03:30,360
+طبعا antigen دخل جسم الإنسان أكيد هيلاقي رد فعل من
+
+42
+00:03:30,360 --> 00:03:35,340
+جهاز المناعة ويكون له أزام مضادة بالتالي لايمكن
+
+43
+00:03:35,340 --> 00:03:41,080
+فصل ال microbiology عن ال immunology في الدراسة
+
+44
+00:03:43,240 --> 00:03:47,740
+طبعا هك اعرفنا شو تعريف علم ال microbiology شو
+
+45
+00:03:47,740 --> 00:03:51,800
+أقسام علم ال microbiology وعرفنا فروع ال medical
+
+46
+00:03:51,800 --> 00:03:56,860
+microbiology الهدف من قسم ال microbiology في
+
+47
+00:03:56,860 --> 00:04:00,940
+المستشفيات يهدف إلى عزل البكتيريا من العينات
+
+48
+00:04:00,940 --> 00:04:05,880
+المرضية وتشخيصها عبر فحوصات مختلفة طيب احنا
+
+49
+00:04:05,880 --> 00:04:10,840
+هنتعامل مع عينات مرضية يعني احتمال كبير يكون فيها
+
+50
+00:04:10,840 --> 00:04:15,280
+pathogenic microorganismبالتالي إجراءات السلامة
+
+51
+00:04:15,280 --> 00:04:19,500
+والوقاية ضرورية أنه نتبعها في معمل الـ
+
+52
+00:04:19,500 --> 00:04:23,880
+Microbiology عشان هيك في أول أسبوع هيكون في عندنا
+
+53
+00:04:23,880 --> 00:04:28,460
+مقدمة المقدمة هتكون عبارة عن safety rules قواعد
+
+54
+00:04:28,460 --> 00:04:33,240
+السلامة داخل معمل الـ Microbiology وهنتكلم أيضًا
+
+55
+00:04:33,240 --> 00:04:38,380
+عن الأدوات في معمل الـ Microbiology Instrument in
+
+56
+00:04:38,380 --> 00:04:42,620
+Microbiology Lab هنتعرف على الأدوات الخاصة بالمعمل
+
+57
+00:04:43,410 --> 00:04:48,170
+الاسبوع التانى هنتكلم عن ال microscope بالنسبة لها
+
+58
+00:04:48,170 --> 00:04:52,770
+ال microscope احنا نتعامل مع micro organism مع
+
+59
+00:04:52,770 --> 00:04:58,510
+كائنات لتراب العين المجردة بالتالي اكيد انا هحتاج
+
+60
+00:04:58,510 --> 00:05:02,830
+ال microscope حتى ان انا اكبرها و اشوفها بشكل واضح
+
+61
+00:05:03,310 --> 00:05:06,750
+في هذا المعمل هنتعرف على الميكروسكوب أجزاء
+
+62
+00:05:06,750 --> 00:05:12,530
+الميكروسكوب وظيفة كل جزء هنتعرف على كيف أننا
+
+63
+00:05:12,530 --> 00:05:16,610
+نستخدم الميكروسكوب وكيف أقدر أجيب ال field بشكل
+
+64
+00:05:16,610 --> 00:05:22,010
+صحيح بالنسبة للأسبوع التالت والأسبوع الرابع
+
+65
+00:05:22,010 --> 00:05:26,430
+والأسبوع الخامس كلها عبارة عنها تكون عن استان على
+
+66
+00:05:26,430 --> 00:05:32,760
+عن صبغاتفي معمل السوائل اللي أخدته فيه الـ Urine
+
+67
+00:05:32,760 --> 00:05:38,620
+Analysis كان في عندي Microscopic Examination وفي
+
+68
+00:05:38,620 --> 00:05:43,040
+عندي من ضمن ال Microscopic Examination في عندي فرع
+
+69
+00:05:43,040 --> 00:05:50,280
+اللي هو البكتيريا لو أنا طبعا جيبت عينة مريض بالـ
+
+70
+00:05:50,280 --> 00:05:54,120
+UTI اللي هو الـ Urinary Tract Infection الديهاب
+
+71
+00:05:54,120 --> 00:05:58,730
+المسالك البوليةهذا المريض لو جبت عينة يورين وعملت
+
+72
+00:05:58,730 --> 00:06:02,050
+لها centrifugation واخدت طبعا اتخلصت من ال
+
+73
+00:06:02,050 --> 00:06:05,730
+supernatant واخدت ال sediment وحطته على slide و
+
+74
+00:06:05,730 --> 00:06:10,490
+cover slip و تحت الميكروسكوب اشي اكيد هشوف تحت
+
+75
+00:06:10,490 --> 00:06:14,870
+الميكروسكوب في عينة اليورين هشوف بكتيريا هذه
+
+76
+00:06:14,870 --> 00:06:20,070
+البكتيريا هشوفها small حجمها صغير و هشوفها
+
+77
+00:06:20,070 --> 00:06:25,240
+transparent شفافةطبعا البكتيريا transparent شفافة
+
+78
+00:06:25,240 --> 00:06:29,100
+مش هقدر اشوفها بشكل واضح كتير هتحتاج لقوة ملاحظة
+
+79
+00:06:29,100 --> 00:06:33,780
+عشان هيك بلجأ للستان للصبغات حتى اني انا اقدر
+
+80
+00:06:33,780 --> 00:06:38,320
+اشوفها بشكل واضح في عندي انا انواع كتيرة من
+
+81
+00:06:38,320 --> 00:06:42,800
+الصبغات و تصنيفات اول شي ان ال simple stand ال
+
+82
+00:06:42,800 --> 00:06:47,770
+simple stand بشكل مختصرهنحكي عن هتحكي الهدف منها
+
+83
+00:06:47,770 --> 00:06:51,950
+اللي هو اني انا اشوف الشاب و ال arrangement شكل و
+
+84
+00:06:51,950 --> 00:06:56,150
+ترتيب الخلايا الخلية البكتيريا بالنسبة
+
+85
+00:06:56,150 --> 00:06:59,930
+لليجرامستان، ليجرامستان يمكن اسمعتوا فيها بتقسم
+
+86
+00:06:59,930 --> 00:07:05,160
+البكتيريا لمجمعتين موجبة جرام و سالبة جرامالاسد
+
+87
+00:07:05,160 --> 00:07:09,940
+فاستستان هي خاصة ببكتيريا السول الرئوي بكتيريا
+
+88
+00:07:09,940 --> 00:07:12,420
+السول الرئوي اللي هي الـ Mycobacterium
+
+89
+00:07:12,420 --> 00:07:17,380
+tuberculosis هذه البكتيريا ما بقدر اني انا اصبغها
+
+90
+00:07:17,380 --> 00:07:24,860
+بالجرام استان تمام فبالتالي هل جاء للاسد فاستستان
+
+91
+00:07:24,860 --> 00:07:31,200
+لصبغتها طبعا هنعرف ايش السبب في عدم مقدرتنا
+
+92
+00:07:31,200 --> 00:07:36,270
+لصبغتها بالجرام استان في خاصيةفي هذه البكتيريا
+
+93
+00:07:36,270 --> 00:07:41,210
+طبعا تتخلين ما نقدر نصبغها بيه لجرام استان
+
+94
+00:07:41,210 --> 00:07:46,130
+بالأسبوع الخامس فيه اننا هنكمل فيه موضوع الاستان
+
+95
+00:07:46,130 --> 00:07:51,230
+فيه اندي انا تراكيب خاصة في الخلية البكتيرية في
+
+96
+00:07:51,230 --> 00:07:58,360
+هذا المعمل هنصبغ special standالستان خاص هتصبغ
+
+97
+00:07:58,360 --> 00:08:02,260
+تراكيب خاصة داخل الخلية البكتيرية فى عندي الـ
+
+98
+00:08:02,260 --> 00:08:06,280
+Spore Stain اللى هى الأبواغ فى حتى إن أنا أعرف هل
+
+99
+00:08:06,280 --> 00:08:10,680
+البكتيريا قادرة على إنتاج الأبواغ أو لأ بستخدم ال
+
+100
+00:08:10,680 --> 00:08:14,360
+Spore Stain الـ Negative Stain اللى هى اسم آخر
+
+101
+00:08:14,360 --> 00:08:16,740
+اللى هى الـ Capsule Stain حتى إن أنا أعرف
+
+102
+00:08:16,740 --> 00:08:20,760
+البكتيريا هل تمتلك Capsule حافظة أو لأ بصبغها بـ
+
+103
+00:08:20,760 --> 00:08:24,620
+Negative Stainbacterial motility عشان اعرف
+
+104
+00:08:24,620 --> 00:08:29,640
+البكتيريا هل هي motile او non motile بستخدم طبعا
+
+105
+00:08:29,640 --> 00:08:35,560
+اللي هي الفلاجلة استان الفلاجلة يعتبر هو طبعا
+
+106
+00:08:35,560 --> 00:08:39,100
+الجزء المسئول عن الحركة اللي هو الصوت فعشان اعرف
+
+107
+00:08:39,100 --> 00:08:44,480
+هل هي motile أو non motile بصبغ الصوت طبعا هذا
+
+108
+00:08:44,480 --> 00:08:48,080
+بالنسبة لأسبوع التالت والرابع والخامس كلها هتكون
+
+109
+00:08:48,080 --> 00:08:53,660
+عبارة عن استانوهنستخدم اكيد المايكروسكوب فبالتالي
+
+110
+00:08:53,660 --> 00:08:57,320
+لازم نكون طالعين من معمل المايكروسكوب عارفين نجيب
+
+111
+00:08:57,320 --> 00:09:01,480
+ال field لأن كل المعامل هتعتمد على المايكروسكوب
+
+112
+00:09:03,340 --> 00:09:07,360
+في الأسبوع السادس هنبدأ نحكي عن ال isolation عزل
+
+113
+00:09:07,360 --> 00:09:11,380
+البكتيريا طيب انا ليش بدي اعزل بكتيريا احنا قلنا
+
+114
+00:09:11,380 --> 00:09:15,400
+هدفنا في قسم ال microbiology اننا نعزل بكتيريا من
+
+115
+00:09:15,400 --> 00:09:20,500
+عينات مرضية و نشخصها عبر فحوصات مختلفة ايش يعني
+
+116
+00:09:20,500 --> 00:09:24,080
+نشخصها يعني بدي اعطي اسم للبكتيريا المسببة
+
+117
+00:09:24,080 --> 00:09:28,800
+للالتهاف بدي اعرف هويتها اللي على أساسه الدكتور
+
+118
+00:09:28,800 --> 00:09:33,180
+هيوصف هلاجة اللي هو ال antibiotic المناسب للمريض
+
+119
+00:09:33,730 --> 00:09:37,830
+هلأ انا في اول معامل لما أخدت العينة وشوفتها تحت
+
+120
+00:09:37,830 --> 00:09:42,150
+ال microscope وصبغتها فقط عرفت انه انا فيها اندي
+
+121
+00:09:42,150 --> 00:09:45,970
+infection او لأ في بكتيريا في العينة او لأ عرفت
+
+122
+00:09:45,970 --> 00:09:50,290
+معلومات قليلة انه هاي مثلا من اي مجموعة ماقدرت
+
+123
+00:09:50,290 --> 00:09:55,470
+اوصل لاسم البكتيرياعشان هيك لازم انا اعزلها من
+
+124
+00:09:55,470 --> 00:10:00,930
+العيينة عشان الصورة تكون اوضح بدنا انشبه البكتيريا
+
+125
+00:10:00,930 --> 00:10:05,970
+بالجنين والعيينة بالام هلأ ال baby طول ما هو طول
+
+126
+00:10:05,970 --> 00:10:11,390
+ما هو بطن امه فقط بقدر اعرف انه هل الام حامل او لأ
+
+127
+00:10:11,390 --> 00:10:16,010
+هل فيه baby او لأ ممكن اعرف مثلا جنسه جنس الجنين
+
+128
+00:10:16,340 --> 00:10:20,840
+لكن تفاصيل أكتر لون بشرته لون عيونه ما بقدر لازم
+
+129
+00:10:20,840 --> 00:10:26,020
+يولد الطفل و أشوفه حتى أقدر أعرف هذه التفاصيل كذلك
+
+130
+00:10:26,020 --> 00:10:31,040
+البكتيريا لازم أعزلها من العينة و أتعامل معها عشان
+
+131
+00:10:31,040 --> 00:10:36,520
+أعرف هذه التفاصيل و أقدر أشخصها بالنسبة للجنين
+
+132
+00:10:36,520 --> 00:10:42,640
+الجنين حتى يولد و يخرج للحياة لازم يموء و يكبر و
+
+133
+00:10:42,640 --> 00:10:47,840
+بالنهاية يتكون جسم إنسان كاملبرضه الجنين بيحتاج
+
+134
+00:10:47,840 --> 00:10:52,280
+حتى ينمو و يكبر لغذاء هذا الغذاء بياخده من طبعا
+
+135
+00:10:52,280 --> 00:10:57,080
+إمه و لازم يكون في بيئة مناسبة لنموه و بيئة مناسبة
+
+136
+00:10:57,080 --> 00:11:01,960
+لحمايته طبعا هذه البيئة هي رحم إمه هنتعامل مع
+
+137
+00:11:01,960 --> 00:11:06,920
+البكتيريا بنفس تعاملنا مع الجنين حتى نقدر نعزلها
+
+138
+00:11:07,270 --> 00:11:12,210
+أول شئ البكتيريا برضه لازم تنمو و تنقسم طبعا
+
+139
+00:11:12,210 --> 00:11:16,550
+البكتيريا تعتبر unicellular يعني وحيدة الخلية
+
+140
+00:11:16,550 --> 00:11:22,170
+بتنقسم بواسطة الانشطار الثنائي و بتنمو بشكل أُسي و
+
+141
+00:11:22,170 --> 00:11:26,250
+بتكون ملايين من الخلايا هذه الخلايا بتتجمع مع
+
+142
+00:11:26,250 --> 00:11:30,630
+بعضها البعض و بتكون ما يعرف بالكلوني الكلوني هو
+
+143
+00:11:30,630 --> 00:11:36,610
+المستعمر هذه مصطلح الكلونيالـ Colony هي عبارة عن
+
+144
+00:11:36,610 --> 00:11:43,750
+مستعمرة هذا طبعا شكل هذه بنسميها Colony مستعمر هذا
+
+145
+00:11:43,750 --> 00:11:48,810
+الشكل يعتبر Colony تجمع من ملايين من الخلايا
+
+146
+00:11:48,810 --> 00:11:52,810
+البكتيريا تجمعت حوالين بعض و كونت في ما يعرف بال
+
+147
+00:11:52,810 --> 00:11:59,500
+Colony طبعا البكتيريا أناما بقدر أشوفها في الواقع
+
+148
+00:11:59,500 --> 00:12:03,380
+إلا على شكل كولوني زي الجنين ما بقدر نشوفه إلا على
+
+149
+00:12:03,380 --> 00:12:07,760
+شكل جسم إنسان كامل تاني الشيء البكتيريا قلنا إنه
+
+150
+00:12:07,760 --> 00:12:13,540
+حتى تنمو بتحتاج لغذاء الغذاء البكتيريا منسمي media
+
+151
+00:12:15,460 --> 00:12:20,660
+media هو الوسط الغذائي للبكتيريا فده المعمل هنتعرف
+
+152
+00:12:20,660 --> 00:12:24,940
+كيف هنحضر ال media كيف هنحضر الوسط الغذائي
+
+153
+00:12:24,940 --> 00:12:29,520
+للبكتيريا طبعا هذا طبق بطري بالنسبة ل media هي
+
+154
+00:12:29,520 --> 00:12:41,180
+المادة الحمراء تمام هي المادة الحمراء لحظة
+
+155
+00:12:44,740 --> 00:12:48,840
+هذه المادة الحمرة تعتبر هي ال media هنتعرف كيف
+
+156
+00:12:48,840 --> 00:12:53,420
+نحضرها طبعا نصبها في طبق البتري داش بعد كده نجيب
+
+157
+00:12:53,420 --> 00:12:56,660
+العينة اللي أنا شاكه في اندي infection الديهاب
+
+158
+00:12:56,660 --> 00:13:01,760
+بزرع هذه العينة و بحطها في جهاز incubator بعد 24
+
+159
+00:13:01,760 --> 00:13:06,980
+ساعة بشوف نمو البكتيريا على شكل مستعمرة تمام؟ طيب
+
+160
+00:13:07,180 --> 00:13:11,840
+بالنسبة للجنين قلنا الجنين طبعا حاضنته الحاضنة
+
+161
+00:13:11,840 --> 00:13:15,760
+الأساسية هي رحم الأم طول فترة التسعة شهور بيكون في
+
+162
+00:13:15,760 --> 00:13:20,200
+رحم الأم طيب البكتيريا حتى تنمو وتتكثر بدها اللي
+
+163
+00:13:20,200 --> 00:13:24,200
+هي حاضنة حاضنت البكتيريا اسمها incubator هذا
+
+164
+00:13:24,200 --> 00:13:28,560
+الجهاز هنتعرف عليه في أول معمل من معامل الـ
+
+165
+00:13:28,560 --> 00:13:33,220
+microbiologyهذه الـ Incubator هي عبارة عن جهاز
+
+166
+00:13:33,220 --> 00:13:37,740
+بوفر الظروف المناسبة لنمو البكتيريا زي ما رحم الأم
+
+167
+00:13:37,740 --> 00:13:41,400
+بوفر الظروف المناسبة لنمو ال baby و ال incubator
+
+168
+00:13:41,400 --> 00:13:47,990
+بوفر الظروف المناسبة لنمو ال babyالبكتيريا بالنسبة
+
+169
+00:13:47,990 --> 00:13:51,810
+للأسبوع السادس قلنا media preparation and
+
+170
+00:13:51,810 --> 00:13:55,470
+sterilization ال sterilization معناها تعقيم to
+
+171
+00:13:55,470 --> 00:14:00,210
+kill all microorganism حتى أني انا اقتل كل ال
+
+172
+00:14:00,210 --> 00:14:04,090
+microorganism التعقيم ال sterilization في شغل ال
+
+173
+00:14:04,090 --> 00:14:08,650
+microbiology مهم جدا جدا لأن ال microorganism
+
+174
+00:14:08,650 --> 00:14:11,710
+موجودة في كل مكان على الأصدفة فياندي microorganism
+
+175
+00:14:11,710 --> 00:14:16,660
+على الجلد فياندي في الهواء فبالتاليعملية التعقيم
+
+176
+00:14:16,660 --> 00:14:20,720
+مهمة جدا هلأ انا لو حضرت ميديا و خلال تحذيري
+
+177
+00:14:20,720 --> 00:14:25,520
+استخدمت flask ماعقمت هذا ال flask بعدها اجيت صبت
+
+178
+00:14:25,520 --> 00:14:29,380
+الميديا في الطبق اللي بترداش و زرعت عليها عينتي
+
+179
+00:14:29,380 --> 00:14:33,920
+بعد 24 ساعة لاقيت في اندي نمو على الطبق طيب هلأ
+
+180
+00:14:33,920 --> 00:14:39,240
+هذا النمو هل هو من العينة ولا هل هو من ال flask
+
+181
+00:14:39,240 --> 00:14:43,440
+اللي انا ماعقمتهفهنا بيصير عندي في لبس في الموضوع
+
+182
+00:14:43,440 --> 00:14:48,860
+فعشان هي في هذا في شغل ال microbiology لازم عملية
+
+183
+00:14:48,860 --> 00:14:52,200
+تعقيم الأدوات تعقيم الشغل فهذا المعمل هنتعرف كيف
+
+184
+00:14:52,200 --> 00:14:56,860
+بنعقم الأدوات كيف بنعقم مكان الشغل بالنسبالي ال
+
+185
+00:14:56,860 --> 00:15:01,040
+aseptic technique هي جميع الإجراءات اللازمة لمنع
+
+186
+00:15:01,040 --> 00:15:05,260
+ال contamination and pure culture concept مش يعني
+
+187
+00:15:05,260 --> 00:15:10,870
+ابيور ابيور معناها نقي ابيور هي معناها نقيطب عشان
+
+188
+00:15:10,870 --> 00:15:16,230
+نفهم أكتر، نكمل في قصة الجنيد، لو كانت الأم حامل
+
+189
+00:15:16,230 --> 00:15:21,810
+بـ Identical Twins وشأت الأقدار أنه يولدوا متصلين،
+
+190
+00:15:21,810 --> 00:15:25,250
+هدول الـ Twins، هل لازم يكون فيه اندي تدخل ضده
+
+191
+00:15:25,250 --> 00:15:30,110
+لفصل التوقم عن بعض؟ لأنه بالنهاية كل واحد له اسم،
+
+192
+00:15:30,110 --> 00:15:35,280
+كل واحد له شخصيةنفس الاشي بالنسبة للبكتيريا لو كان
+
+193
+00:15:35,280 --> 00:15:40,100
+عندي infection لو كان عندي التهاب وكان سبب الاتهاب
+
+194
+00:15:40,100 --> 00:15:45,280
+أكتر من microorganism هنلاحظ بعد 24 ساعة نمو
+
+195
+00:15:45,280 --> 00:15:49,000
+مستعمرات على الطبق في مستعمرات هتكون مخدة شكل معين
+
+196
+00:15:49,000 --> 00:15:53,080
+ومستعمرات تانية مخدة شكل مختلف هان هحس ان في عندي
+
+197
+00:15:53,080 --> 00:15:56,780
+إذا الكله مش نفس الشكل هعرف ان في عندي two
+
+198
+00:15:56,780 --> 00:16:03,380
+microorganismفلازم هان اخد كل colony كل شكل مثلا
+
+199
+00:16:03,380 --> 00:16:09,000
+الشكل ال colony كل شكل من هدول الشكلين اخد و
+
+200
+00:16:09,000 --> 00:16:13,680
+ازرعهم كل واحدة منهم على طبق على احدى عشان تكون
+
+201
+00:16:13,680 --> 00:16:19,000
+المزرعة عندى pure حتى يكون اصل ال colony عبارة عن
+
+202
+00:16:19,000 --> 00:16:23,740
+خلية بكتيرية واحدة وهذا الاشي ضروري جدا للفحوصات
+
+203
+00:16:23,740 --> 00:16:28,050
+الكيميائيةبالنسبة لآخر إشي الـ Colony Morphology
+
+204
+00:16:28,050 --> 00:16:32,650
+اللي هو شكل المستعمرات على الطبق ممكن من خلال شكل
+
+205
+00:16:32,650 --> 00:16:36,810
+المستعمرات على الطبق أتعرف على اللي هو هوية
+
+206
+00:16:36,810 --> 00:16:40,650
+البكتيريا في بكتيريا عندنا اسمها Streptococcus من
+
+207
+00:16:40,650 --> 00:16:46,550
+مميزات هذه البكتيريا أنه أنا على طبعا شكل
+
+208
+00:16:46,550 --> 00:16:50,370
+المستعمرات هذه البكتيريا على الطبق بتكون pinpoint
+
+209
+00:16:50,370 --> 00:16:54,590
+على شكل راس الدبوسي كتير حجمها صغيرفأنا من حد ما
+
+210
+00:16:54,590 --> 00:16:59,050
+أشوف pinpoint على الطبق احتمالي كبير بحكي انه high
+
+211
+00:16:59,050 --> 00:17:04,070
+streptolacin لازم نأكد ال .. طبعا نأكد اللي هو
+
+212
+00:17:04,070 --> 00:17:12,230
+التشخيص بفكوثات كيميائية هذا بالنسبة لأسبوع السادس
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/fiNRLFNYLh8_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/fiNRLFNYLh8_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..18ab9c8af115185523cfcfe223aa32c249436b51
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/fiNRLFNYLh8_postprocess.srt
@@ -0,0 +1,1648 @@
+1
+00:00:02,330 --> 00:00:06,610
+Methyl redvocas-buscowar test هذا الفحص له علاقة
+
+2
+00:00:06,610 --> 00:00:09,990
+بالبكتيريا ال fermentation لو البكتيريا عملت
+
+3
+00:00:09,990 --> 00:00:14,710
+fermentation للجليكوز هعمل فحص الـ Methyl redvocas
+
+4
+00:00:14,710 --> 00:00:18,950
+-buscowar قولنا إن من نتائج عملية ال fermentation
+
+5
+00:00:18,950 --> 00:00:24,210
+ال end product إما يكون أسد أو الكحول وقلنا إن ال
+
+6
+00:00:24,210 --> 00:00:27,210
+end product هي من خلالها أنا بقدر أعمل
+
+7
+00:00:27,210 --> 00:00:30,570
+identification للبكتيرياكيف بدي اعمل
+
+8
+00:00:30,570 --> 00:00:33,890
+identification للبكتيريا من خلال الـ end product
+
+9
+00:00:33,890 --> 00:00:38,470
+من خلال فحص الـ methyl red vocas buscala عشان نعرف
+
+10
+00:00:38,470 --> 00:00:49,090
+أكتر لو
+
+11
+00:00:49,090 --> 00:00:52,810
+البكتيريا عملت fermentation للجليكوز عملت فحص
+
+12
+00:00:52,810 --> 00:00:57,070
+البكتيريا ال fermentation اللي شرحناه في الجزء
+
+13
+00:00:57,070 --> 00:01:01,580
+الأولبقول لي بتعمل فحص الـ Methyl Red Vagas
+
+14
+00:01:01,580 --> 00:01:05,160
+Buscowar Test الـ Methyl Red Vagas Buscowar Test
+
+15
+00:01:05,160 --> 00:01:09,980
+هذا من خلاله بدي أعرف أي pathway سلكت البكتيريا
+
+16
+00:01:09,980 --> 00:01:15,580
+البكتيريا لما تنتج acid بقول لي بتسلق ال pathway
+
+17
+00:01:15,580 --> 00:01:19,600
+اسمه mixed acid fermentation يبقى لو كان ناتج
+
+18
+00:01:19,600 --> 00:01:24,480
+عملية ال fermentation acid بتكون البكتيريا سلكت ال
+
+19
+00:01:24,480 --> 00:01:28,870
+pathway اللي اسمه mixed acid fermentationلو كان
+
+20
+00:01:28,870 --> 00:01:33,070
+ناتج عملية ال fermentation ألكحول بتكون البكتيريا
+
+21
+00:01:33,070 --> 00:01:38,470
+سلكت ال pathway اللي اسمه butylene glycol pathway
+
+22
+00:01:38,470 --> 00:01:44,750
+or neutral pathway or butanediol pathway تلت أسامي
+
+23
+00:01:44,750 --> 00:01:51,510
+ل ال pathway هذا اللي نواتج ال fermentation بتكون
+
+24
+00:01:51,510 --> 00:01:56,480
+ألكحول بالنسبة ل ال mixed acid fermentationأنا
+
+25
+00:01:56,480 --> 00:02:01,020
+عشان أكشف هل البكتيريا سلكت هذا ال pathway بستخدم
+
+26
+00:02:01,020 --> 00:02:06,560
+فحص الـ Methyl Red Test عشان أعرف البكتيريا سلكت
+
+27
+00:02:06,560 --> 00:02:10,940
+ال pathway اللي اسمه neutral pathway او بيوتانديول
+
+28
+00:02:10,940 --> 00:02:16,580
+او بيوتيولين ايش اللي يقول pathway بستخدم فحص ال
+
+29
+00:02:16,580 --> 00:02:20,640
+AUGUST BUSCUAR TEST بالنسبة لـMixed Acid
+
+30
+00:02:20,640 --> 00:02:23,980
+Fermentation قلنا ان البكتيريا بتعمل ferment
+
+31
+00:02:23,980 --> 00:02:29,280
+للجليكوزand produce extreme amount of acid قولنا
+
+32
+00:02:29,280 --> 00:02:32,720
+نواتج العملية في هذا ال pathway acid بقول extreme
+
+33
+00:02:32,720 --> 00:02:37,460
+amount of acid بتكون ال acid strong acid زي ال
+
+34
+00:02:37,460 --> 00:02:41,160
+formic acid الacidic acid و ال lactic acid هاي ال
+
+35
+00:02:41,160 --> 00:02:46,060
+acid تعتبر strong acid فبالتالي هتعمل على خفط ال
+
+36
+00:02:46,060 --> 00:02:52,140
+pH بشكل كبير high decrease in pH أقل من خمسةالـ pH
+
+37
+00:02:52,140 --> 00:02:57,500
+بوصل فبالتالي بيعمل على تغيير لون ال media في
+
+38
+00:02:57,500 --> 00:03:02,040
+الفحص ال bacterial fermentation لو عملته بشكل بشكل
+
+39
+00:03:02,040 --> 00:03:06,380
+كبير بيعمل على تغيير ال pH بيخفض ال pH بشكل كبير
+
+40
+00:03:06,380 --> 00:03:10,160
+عشان هيك بفحص ال bacterial fermentation اللي حكينا
+
+41
+00:03:10,160 --> 00:03:16,320
+عنهكان في لتيوب لون اللي لتيوب كان أصفر غامق أصفر
+
+42
+00:03:16,320 --> 00:03:20,380
+غامق قلنا لأنه بتنتج كمية كبيرة من ال acid strong
+
+43
+00:03:20,380 --> 00:03:24,780
+acid يبقى كانت في لتيوب هاي ال pathway mixed acid
+
+44
+00:03:24,780 --> 00:03:29,320
+pathway كان extreme amount of acid بقولي نواتج
+
+45
+00:03:29,320 --> 00:03:33,000
+عملية ال fermentation في ال mixed acid
+
+46
+00:03:33,000 --> 00:03:38,300
+fermentation بتكون stable عشان أكشف عن هاي ال
+
+47
+00:03:38,300 --> 00:03:42,720
+pathwayبضيف reagent اسمه missile red reagent
+
+48
+00:03:42,720 --> 00:03:45,780
+هنتعرف على ال procedure ومتى انا هضيفها ده ال
+
+49
+00:03:45,780 --> 00:03:51,380
+missile red بالنسبالى ال neutral pathway او
+
+50
+00:03:51,380 --> 00:03:55,080
+butanediol pathway او butylene glycol pathway
+
+51
+00:03:55,080 --> 00:03:58,820
+بقوللي ان البكتيريا بتعمل fermentation للجليكوز
+
+52
+00:03:58,820 --> 00:04:04,840
+لكن بتنتج الكحول اللي هو الacetoneالاسيتون يعتبر
+
+53
+00:04:04,840 --> 00:04:09,180
+الكحول من نواتج عملية ال fermentation هذا الاسيتون
+
+54
+00:04:09,180 --> 00:04:14,060
+يعتبر unstable على عكس ال strong acid كانت هنا في
+
+55
+00:04:14,060 --> 00:04:18,060
+ال mixed acid pathway stable لكن الاسيتون يعتبر
+
+56
+00:04:18,060 --> 00:04:23,200
+unstable acidic product او هو يعتبر acidic لكن مش
+
+57
+00:04:23,200 --> 00:04:27,380
+strong acid هيعمل على خفض ال pH لكن مش بصورة كبيرة
+
+58
+00:04:27,380 --> 00:04:32,460
+هيكون slight decrease in pHبقول لي بما ان هو
+
+59
+00:04:32,460 --> 00:04:38,620
+unstable اكيد عشان يصير مستقر هيتحول لمركب اكثر
+
+60
+00:04:38,620 --> 00:04:43,920
+استقرارا هيتحول لبيوتان ديول هذا البيوتان ديول
+
+61
+00:04:43,920 --> 00:04:48,640
+stable عشان هيك سميت ال pathway اسمه بيوتان ديول
+
+62
+00:04:48,640 --> 00:04:52,960
+pathwayهذا المُركّب بقول لي neutral product عشان
+
+63
+00:04:52,960 --> 00:04:55,900
+هيك سميت ال pathway اسم تاني كان ال neutral
+
+64
+00:04:55,900 --> 00:04:59,880
+pathway يبقى هيعمللي slight decrease in pH عشان
+
+65
+00:04:59,880 --> 00:05:04,960
+هيك ال tube كانت في الجزء القبل في المحاضرة لما
+
+66
+00:05:04,960 --> 00:05:09,220
+كشفت عن ال fermentation كان اللون فاتح اتكون
+
+67
+00:05:09,220 --> 00:05:13,100
+القحول عمللي على خفض ال pH لكن كان slight decrease
+
+68
+00:05:13,100 --> 00:05:19,910
+in pHعشان اوضح الصورة اكتر او ان انا اعرف هل هي
+
+69
+00:05:19,910 --> 00:05:23,990
+سلكة الـVB pathway اللي هو الـbutanediol pathway
+
+70
+00:05:23,990 --> 00:05:27,510
+او الـmixed acid pathway لازم اعمل فحص
+
+71
+00:05:27,510 --> 00:05:30,930
+الـmethyredvo-casposcavar لان اوقات ممكن مايكون في
+
+72
+00:05:30,930 --> 00:05:34,930
+عندي الـtube ان انا اقارن الفرق في اللون هل هو
+
+73
+00:05:34,930 --> 00:05:38,110
+غامق او فاتح ممكن تطلع كلها البكتيريا اللي عندي
+
+74
+00:05:38,110 --> 00:05:41,910
+سلكة طريقة الـmixed acid pathwayفبالتالي مش هقدر
+
+75
+00:05:41,910 --> 00:05:45,310
+اني انا افرد فعشان هيك عملوا فحص الـ Methyl Red
+
+76
+00:05:45,310 --> 00:05:49,550
+Vukasposkaur الـ Reagent اللي انا بدي اضيفه عشان
+
+77
+00:05:49,550 --> 00:05:53,230
+اكشف على هذا ال pathway اسمه Barrett's Reagent الـ
+
+78
+00:05:53,230 --> 00:05:57,870
+Barrett's Reagent بتكون من VB1 VB نتيجة اختصار ل
+
+79
+00:05:57,870 --> 00:06:02,270
+ال V اسم الفحص اللي هو Vukasposkaurالأول الـ
+
+80
+00:06:02,270 --> 00:06:05,070
+reagent اللى أنا بضيفه اللى هو خمسة في المية Alpha
+
+81
+00:06:05,070 --> 00:06:10,310
+-Naphthol VB2 اللى بدي أضيفه بعد ما ضفت الأول هضيف
+
+82
+00:06:10,310 --> 00:06:17,770
+reagent أربعين في المية KOH KOH هو هذا ال VB2 يبقى
+
+83
+00:06:17,770 --> 00:06:22,110
+البرتز reagent بتكون من VB1 و VB2 ال VB1 Alpha
+
+84
+00:06:22,110 --> 00:06:28,110
+-Naphthol ال VB2 أربعين في المية KOH طيب كيف أنا
+
+85
+00:06:28,110 --> 00:06:34,330
+بديأكشف على ال path way أي path way سلكة البكتيريا
+
+86
+00:06:34,330 --> 00:06:40,110
+بقوللي اني بدي اجيب media اسمها methyl red vucus
+
+87
+00:06:40,110 --> 00:06:43,090
+puscawar هاي ال media بتكون ال broth الميديا
+
+88
+00:06:43,090 --> 00:06:48,390
+بتحتوي على جيليكوز اكيد ك substrate هيشتغل عليها
+
+89
+00:06:48,390 --> 00:06:52,790
+هتشتغل عليها البكتيريا هتعمل fermentation لجيليكوز
+
+90
+00:06:52,790 --> 00:06:58,410
+في عندى في الميديا برضه ببطونمصدر للـ nitrogen
+
+91
+00:06:58,410 --> 00:07:02,550
+source عشان تنمو البكتيريا بولي هاي ال media
+
+92
+00:07:02,550 --> 00:07:07,470
+بتاخدي two tube منها واحد ال tube هيكون لفحص ال
+
+93
+00:07:07,470 --> 00:07:11,250
+methyl red test وال tube التاني هيكون لفحص ال VB
+
+94
+00:07:11,250 --> 00:07:17,980
+testنفس السبسترات لأن نفس السبسترات هيشتغلوا عليها
+
+95
+00:07:17,980 --> 00:07:22,420
+فبالتالي هتكون نفس ال media في كيلاء الفحصين لكن
+
+96
+00:07:22,420 --> 00:07:26,040
+الاختيلة في ال reagent اللي انا هضيفه بعد ال
+
+97
+00:07:26,040 --> 00:07:29,380
+incubation بعد ال incubation لفحص ال methyl red
+
+98
+00:07:29,380 --> 00:07:32,580
+هضيف ال reagent اللي هو قولنا methyl red reagent
+
+99
+00:07:32,580 --> 00:07:36,540
+لكن لفحص ال focus بسكوت test هضيف ال barretts
+
+100
+00:07:36,540 --> 00:07:41,820
+reagent يبقى انا بستخدمهدول الفحصين باستخدام لهم
+
+101
+00:07:41,820 --> 00:07:45,120
+نفس ال media هاي ال media بتحتوي على جليكوز ك
+
+102
+00:07:45,120 --> 00:07:49,120
+substrate بقول لي نفس ال organism بدي أزرعه في
+
+103
+00:07:49,120 --> 00:07:53,680
+توقت يوب هعمل incubation لمدة 24 ساعة على درجة
+
+104
+00:07:53,680 --> 00:07:57,760
+حرارة 37 بعد ما تخلص فترة ال incubation و تم
+
+105
+00:07:57,760 --> 00:08:02,820
+البكتيريا أكيد هتكون عاملة fermentation لجليكوز
+
+106
+00:08:02,820 --> 00:08:06,420
+تمام أنا بالشكل المبدأي عرفت أنه هي جليكوز
+
+107
+00:08:06,420 --> 00:08:10,100
+fermenter من الفحص الليحكينا عنه في الجزء الاول
+
+108
+00:08:10,100 --> 00:08:13,540
+هلا بدي اعرف اي pathway سلكة ال mixed acid pathway
+
+109
+00:08:13,540 --> 00:08:16,000
+او بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان
+
+110
+00:08:16,000 --> 00:08:17,280
+بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان
+
+111
+00:08:17,280 --> 00:08:24,760
+بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان
+
+112
+00:08:24,760 --> 00:08:27,780
+بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان
+
+113
+00:08:27,780 --> 00:08:32,120
+بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان بيوتان
+
+114
+00:08:32,120 --> 00:08:37,590
+بهيعمل على خفض ال pH بصورة كبيرة high decrease in
+
+115
+00:08:37,590 --> 00:08:42,590
+pH هضيف اكيد pH indicator بقول ال pH indicator في
+
+116
+00:08:42,590 --> 00:08:47,990
+هذا الفحص هو ال methyl red مش بيكون موجود في ال
+
+117
+00:08:47,990 --> 00:08:51,430
+media انا بضيفه بعد ما اتخلص incubation بعد ما
+
+118
+00:08:51,430 --> 00:08:54,570
+اتخلص فترة ال incubation بضيف ال pH indicator
+
+119
+00:08:54,570 --> 00:08:59,270
+بيكون ال reagent لونه أحمر بضيف two drop من one ل
+
+120
+00:08:59,270 --> 00:09:05,210
+three drop على ال tubeالميثايرد رياجنت شو حدوده
+
+121
+00:09:05,210 --> 00:09:09,610
+بيقول لي بيكون في ال acidic لونه أحمر وفي ال basic
+
+122
+00:09:09,610 --> 00:09:14,170
+بيكون لونه أصفر لنيوترال بيكون ما بين 4 و 4 و 6 و
+
+123
+00:09:14,170 --> 00:09:18,570
+2 لونه orange ننتبه بالفحص اللي اتكلمنا عنه بالأول
+
+124
+00:09:18,570 --> 00:09:21,090
+ال fermentation عشان أشوف بشكل عام هل هي بتعمل
+
+125
+00:09:21,090 --> 00:09:26,510
+fermentation ولا لأ كانالـ pH Indicator في نول رد
+
+126
+00:09:26,510 --> 00:09:28,770
+وكان موجود في ال media أنا ماضفته بعد ال
+
+127
+00:09:28,770 --> 00:09:32,670
+incubation الحدود معاكوسة كانت هناك في ال acidic
+
+128
+00:09:32,670 --> 00:09:37,130
+yellow وفي ال basic لون red لكن هنا في ال acidic
+
+129
+00:09:37,130 --> 00:09:42,230
+لونه أحمر وفي ال basic لون yellow يبقى اتكون عندي
+
+130
+00:09:42,230 --> 00:09:46,710
+من نتيجة ال fermentation strong acid هتعمل على خفض
+
+131
+00:09:46,710 --> 00:09:53,960
+ال pH بصورة كبيرة هتكون أقل من 4.4هيتحول اللون
+
+132
+00:09:53,960 --> 00:09:57,120
+اللون للون red يبقى النتيجة ال positive هان red
+
+133
+00:09:57,120 --> 00:10:00,180
+كانت هناك في ال fermentation بالبكتيريا ال
+
+134
+00:10:00,180 --> 00:10:03,920
+fermentation كانت النتيجة ال yellow كانت هي ال
+
+135
+00:10:03,920 --> 00:10:09,180
+positive لأن ال indicator بيختلف لون ال media
+
+136
+00:10:09,180 --> 00:10:13,700
+لونها أصفر اللي هنزرع عليها فلو ما تغير أي شيء ضال
+
+137
+00:10:13,700 --> 00:10:16,760
+لون ال media لونه yellow يبقى هي negative من
+
+138
+00:10:16,760 --> 00:10:20,620
+الأمثلة على ال positive ال E.coli و ال Brutias
+
+139
+00:10:20,620 --> 00:10:24,350
+mirabilisمن الأمثلة على الـ Negative
+
+140
+00:10:24,350 --> 00:10:27,970
+الكليبسيلانيومانيا و الـ Enterobacter يبقى هذا
+
+141
+00:10:27,970 --> 00:10:32,390
+الفحص الـ Methyl Red Vox Biscuit يستخدم لتفريق
+
+142
+00:10:32,390 --> 00:10:36,010
+الكوليفارم بكتيريا قلنا الكوليفارم بكتيريا اللي هي
+
+143
+00:10:36,010 --> 00:10:40,110
+الـ E.coli الستروبكتر الـ Enterobacter و
+
+144
+00:10:40,110 --> 00:10:43,350
+الكليبسيلة عشان أفرق بينهم أخدنا فحص السيطرات
+
+145
+00:10:43,350 --> 00:10:46,770
+بقولي برضه بتستخدمي فحص الـ Methyl Red Vox Biscuit
+
+146
+00:10:46,770 --> 00:10:51,350
+و هنستخدم برضه فحص الاندول هذا فيما يسمى بـ MVEC
+
+147
+00:10:51,660 --> 00:10:55,020
+الـ M-Vecto استخدم لتمييز الكوليفورم بش تغير
+
+148
+00:10:55,020 --> 00:10:58,420
+الأربع فحوصات عشان أفرق الكوليفورم بكتيريا
+
+149
+00:10:58,420 --> 00:11:01,240
+الإكليبسيلا السيتروباكتر الانتيروباكتر وال E-coli
+
+150
+00:11:01,240 --> 00:11:05,760
+هذا بالنسبة لـ Methyl Red بالنسبة للـ Vocus
+
+151
+00:11:05,760 --> 00:11:08,840
+Biscuit Test بعد ما أنا أعمل incubation و أتخلص
+
+152
+00:11:08,840 --> 00:11:12,520
+فترة ال incubation عشان أكشف عنه بضيف البرتز
+
+153
+00:11:12,520 --> 00:11:15,900
+رياجون نفس الفكرة هضيف البرتز رياجون بعد ال
+
+154
+00:11:15,900 --> 00:11:21,480
+incubation وقوللي بتضيفي VB1 بعدين بتضيفي VB2ممنوع
+
+155
+00:11:21,480 --> 00:11:27,160
+أنا أبدل هنحكي السبب بعد ما نشرح كيف أو إيهش
+
+156
+00:11:27,160 --> 00:11:32,780
+التفاعل اللي بيصير قولنا من نواتج عملية ال ال
+
+157
+00:11:32,780 --> 00:11:36,400
+pathway اللي هو الـ butanediol pathway أسيتوين
+
+158
+00:11:36,400 --> 00:11:40,760
+الأسيتوين هو ألكحول قولنا ألكحول الأمثلة عليها
+
+159
+00:11:40,760 --> 00:11:45,520
+الأسيتوين الأسيتوين يعتبر acidic لكن ما بيعمل على
+
+160
+00:11:45,520 --> 00:11:51,000
+خفض الـ pH بصورة كبيرة UnstableReagent VB1 اللي هو
+
+161
+00:11:51,000 --> 00:11:55,000
+الـ Alpha-Naphthol برتبط في الـ Acetone وبعطيني
+
+162
+00:11:55,000 --> 00:11:59,280
+Complex بعدها أنا هضيف بعدى بمدة تقريبا 10 دقايق
+
+163
+00:11:59,280 --> 00:12:06,240
+هضيف الـ VB2 الـ VB2 اللي هو الـ Hydrogen الـ KOH
+
+164
+00:12:06,240 --> 00:12:10,940
+الـ KOH هيرتبط بهذا الكمplex ويعطينى اللون الـ Red
+
+165
+00:12:10,940 --> 00:12:15,580
+يبقى النتيجة الـ Positive هتكون Red لكن النتيجة
+
+166
+00:12:15,580 --> 00:12:18,000
+الـ Negative مش هيصير تغير في الميديا لون الميديا
+
+167
+00:12:18,000 --> 00:12:22,560
+لونها أصفرالبكتيريا الـ positive لهذا الفحص
+
+168
+00:12:22,560 --> 00:12:25,480
+كليبسيلا نيومانيا و Enterobacter لكن الـ negative
+
+169
+00:12:25,480 --> 00:12:29,520
+الـ E.coli و البروتياس ميرابيلاس نلاحظ أن اللي
+
+170
+00:12:29,520 --> 00:12:33,580
+كانت positive في الـ methyl red هتكون negative في
+
+171
+00:12:33,580 --> 00:12:36,180
+الـ Vagus Buscal و اللي كانت negative في الـ
+
+172
+00:12:36,180 --> 00:12:40,800
+methyl red هتكون positive في الـ Vagus Buscal يبقى
+
+173
+00:12:40,800 --> 00:12:44,800
+هذا الفحص أكيد البكتيريا اللي سلكت الـ mixed acid
+
+174
+00:12:44,800 --> 00:12:49,560
+pathwayمش هتسلق الطريق التاني إما هتسلق طريق الـ
+
+175
+00:12:49,560 --> 00:12:53,040
+mixed acid pathway أو طريق اللي هو الـ butanediol
+
+176
+00:12:53,040 --> 00:12:57,380
+pathway مش هتسلق اتينين مع بعض يبقى بعمل الفحص الـ
+
+177
+00:12:57,380 --> 00:13:00,660
+methyl red لبكتيريا الـ E.coli إش طبيعي يكون الـ
+
+178
+00:13:00,660 --> 00:13:03,880
+vascular اللي لها ال E.coli بما أنها كانت positive
+
+179
+00:13:03,880 --> 00:13:06,500
+في ال methyl red إش طبيعي هيكون negative في ال E
+
+180
+00:13:06,500 --> 00:13:12,780
+.coli لأنها تسلق فقط pathway واحد لكنلو طلعت نيجة
+
+181
+00:13:12,780 --> 00:13:16,400
+في الـ Methyl Red فأكيد هتكون سالكة الطريق التاني
+
+182
+00:13:16,400 --> 00:13:18,900
+اللي هو الـ butanediol فهتكون positive في الـ
+
+183
+00:13:18,900 --> 00:13:24,460
+focus بوسكوية هذا الفحص كيف يشتغل الفحص و كيف
+
+184
+00:13:24,460 --> 00:13:28,380
+نشتغل الفحص و الـ principle لهذا الفحص أنه في عندي
+
+185
+00:13:28,380 --> 00:13:33,800
+Methyl Red بيعمل drop في الـ mixed acid pathway
+
+186
+00:13:33,800 --> 00:13:37,520
+فيه بيكون عندي high decrease in pH فبالتالي هيتحول
+
+187
+00:13:37,520 --> 00:13:43,080
+لون الـ pH indicator للون أحمريلكن هنا مافي NDPH
+
+188
+00:13:43,080 --> 00:13:47,640
+Indicator هضيف Reagent اللي هو الـ VB1 و VB2 الـ
+
+189
+00:13:47,640 --> 00:13:52,020
+VB1 برتبط مع الـ Acetone انتباه وليس البيوتانديون
+
+190
+00:13:52,020 --> 00:13:55,660
+مع الـ Acetone الـ Unstable هيعطيني بالنهاية
+
+191
+00:13:55,660 --> 00:13:58,540
+Complex هذا الـ Complex هيرتبط بالـ VB2 هيعطيني
+
+192
+00:13:58,540 --> 00:14:01,900
+اللون الـ Red يبقى اللون الـ Red نتج مش نتيجة انه
+
+193
+00:14:01,900 --> 00:14:05,600
+كان في NDPH Indicator نتيجة Reagent اتفاعل مع
+
+194
+00:14:05,600 --> 00:14:11,000
+نواتج التفاعليبقى ارتبط مع نواتج التفاعل الـ
+
+195
+00:14:11,000 --> 00:14:14,380
+Acetyl-Nucleotanyl-1 مش نتيجة الـ pH Integrator أو
+
+196
+00:14:14,380 --> 00:14:20,740
+تغيير في الـ pH فيها نقطتين مهمين لازم نتبهلقلهم
+
+197
+00:14:20,740 --> 00:14:26,640
+انه انا بعمل VB1 بحط بعدين بحط VB2 ممنوع احط VB2
+
+198
+00:14:26,640 --> 00:14:31,000
+بعدين احط VB1 السؤال هان why او شو هيصير لو انا
+
+199
+00:14:31,000 --> 00:14:37,830
+حطيت VB2أول issue بعدين حطيت VB1 بقول لي ال VB2 لو
+
+200
+00:14:37,830 --> 00:14:43,250
+حطيتيه بالأول مش هيرتبط مع ال Acetone تمام ال
+
+201
+00:14:43,250 --> 00:14:48,490
+Acetone فقط هيرتبط مع VB1 ال VB2 لو حطيتيه بالأول
+
+202
+00:14:48,490 --> 00:14:51,850
+مش هيرتبط بال Acetone هيروح يرتبط بال Peptone اللي
+
+203
+00:14:51,850 --> 00:14:56,000
+موجود في ال mediaلما يرتبط مع البيبتون الموجود في
+
+204
+00:14:56,000 --> 00:15:03,280
+ال media هيعطيني لون red فانا هفكر انه النتيجة
+
+205
+00:15:03,280 --> 00:15:06,940
+positive تمام فبالتالي هيعطيني false positive
+
+206
+00:15:06,940 --> 00:15:11,720
+result كان اللون ال red نتيجة ارتباط ال VB2KOH مع
+
+207
+00:15:11,720 --> 00:15:15,740
+البيبتون في ال media وليس نتيجة ارتباط ال reagent
+
+208
+00:15:15,740 --> 00:15:21,200
+مع الacetoneعشان هيك لازم احنا نحط VB1 أول بعدين
+
+209
+00:15:21,200 --> 00:15:25,940
+VB2 لو حطيت VB2 هيرتبط مع البيبتون مش هيرتبط مع ال
+
+210
+00:15:25,940 --> 00:15:30,240
+Acetone لأن ال Acetone فقط مخصص انه يرتبط مع VB1
+
+211
+00:15:30,240 --> 00:15:33,720
+هيرتبط VB2 مع البيبتون و هيعطيني اللون ال Red انا
+
+212
+00:15:33,720 --> 00:15:37,000
+هفكره انه هو النتيجة ال positive لكن هو مش النتيجة
+
+213
+00:15:37,000 --> 00:15:41,640
+ال positive هو false positive result النقطة
+
+214
+00:15:41,640 --> 00:15:47,590
+التانية المهمة في هذا الفحص انه انا لازمأقرأ
+
+215
+00:15:47,590 --> 00:15:53,590
+النتيجة بعد ما ازدش الفترة اني احط ال reagent بعد
+
+216
+00:15:53,590 --> 00:16:00,990
+72 ساعة 24 ساعة طلعتها من ال incubator ممنوع بعد
+
+217
+00:16:00,990 --> 00:16:06,050
+72 ساعة احط ال reagent هيعطيني false negative
+
+218
+00:16:06,050 --> 00:16:12,410
+result ليش؟ لأن ال acetone قلنا unstable عشان يصير
+
+219
+00:16:12,410 --> 00:16:17,210
+مستقر بيتحول ل butanediolلـ Butanediol Stable
+
+220
+00:16:17,210 --> 00:16:21,290
+فبالتالي بعد 72 ساعة بيكون الـ Acetone يتحول لـ
+
+221
+00:16:21,290 --> 00:16:24,570
+Butanediol لو أنا ضفت الرياجانت الرياجانت قلنا
+
+222
+00:16:24,570 --> 00:16:28,810
+بيرتبط فقط مع الـ Acetone فبالتالي مش هيلاقي الـ
+
+223
+00:16:28,810 --> 00:16:32,610
+Acetone لأنه اتحول لـ Butanediol فهيعطيني negative
+
+224
+00:16:32,610 --> 00:16:35,550
+result مش هيتحول اللون للون Red هيكون not changed
+
+225
+00:16:35,550 --> 00:16:39,350
+في اللون الميديا فبالتالي هيعطي false negative
+
+226
+00:16:39,350 --> 00:16:45,150
+result عشان هيك لازم انيأنا أتكون المزرعة أخد أو
+
+227
+00:16:45,150 --> 00:16:50,850
+أضيف الـReagent بعد 24 ساعة ما تزيد عن 2 مش بعد 72
+
+228
+00:16:50,850 --> 00:16:55,450
+ساعة لأن الـAcetone unstable هيتحول لـButanediol
+
+229
+00:16:55,450 --> 00:16:59,270
+والـReagent فقط بيرتبط مع الـAcetone وليس مع
+
+230
+00:16:59,270 --> 00:17:07,170
+الـButanediol فهيعطيني False Negative Result طيب
+
+231
+00:17:07,640 --> 00:17:10,380
+قلنا لو البكتيريا عملت fermentation للـGelicose
+
+232
+00:17:10,380 --> 00:17:12,740
+عملت فحص البكتيريا الـfermentation اللي اتكلمنا
+
+233
+00:17:12,740 --> 00:17:16,680
+عنه في الجزء الأول اللي هو اللي فيه فيه نار Red
+
+234
+00:17:16,680 --> 00:17:21,340
+وGelicose والدرهامي Tube هذا الفحص لو أنا عملته
+
+235
+00:17:21,340 --> 00:17:26,540
+وطلعت positive بعمل فحص الـmixed methyl red vucose
+
+236
+00:17:26,540 --> 00:17:31,660
+buscowar test لو طلعت methyl red positiveوقص
+
+237
+00:17:31,660 --> 00:17:34,580
+مستكور نيجاتيف يبقى هي بتكون سلكت ال pathway ال
+
+238
+00:17:34,580 --> 00:17:37,720
+mixed acid pathway اللي هو ناتج عملية ال
+
+239
+00:17:37,720 --> 00:17:42,160
+fermentation strong acid من الأمثلة على هذا ال
+
+240
+00:17:42,160 --> 00:17:46,940
+positive للميثايرد و negative للوقص مستكور ال E
+
+241
+00:17:46,940 --> 00:17:52,620
+.coli وقلنا كمان لبروتيا ميرابيلسلكن لو كانت
+
+242
+00:17:52,620 --> 00:17:55,940
+Methyl Red Negative و Vagas Buscar Positive يعني
+
+243
+00:17:55,940 --> 00:18:00,560
+سلكة طريق لبيوتيولين إجلايكول pathway من الأمثلة
+
+244
+00:18:00,560 --> 00:18:04,820
+على انها تكون Methyl Red Negative Vagas Buscar
+
+245
+00:18:04,820 --> 00:18:08,370
+Positive لك لبسلة و NT رابعة أكتربقول لي لو
+
+246
+00:18:08,370 --> 00:18:12,210
+البكتيريا ما عملت fermentation بالاصل للجليكوز انا
+
+247
+00:18:12,210 --> 00:18:15,250
+ممنوع ان انا اعمل اصلا فحص الـ Methyl Red-Vacous
+
+248
+00:18:15,250 --> 00:18:19,910
+-Biscuit لان لو عملت الفحص لو انا عملت الفحص هيطلع
+
+249
+00:18:19,910 --> 00:18:23,390
+negative للـ Methyl Red و negative للVacous
+
+250
+00:18:23,390 --> 00:18:26,770
+-Biscuit زي الصدومونس الصدومونس بالاصل ما بتعمل
+
+251
+00:18:26,770 --> 00:18:29,630
+fermentation جليكوز يبقى ولا هيتكون acid ولا
+
+252
+00:18:29,630 --> 00:18:34,090
+هيتكون الجحول ممنوع انا اشوف نتيجة ان الاتنين
+
+253
+00:18:34,090 --> 00:18:36,370
+positive ال Methyl Red وVacous-Biscuit لو ما
+
+254
+00:18:36,370 --> 00:18:40,170
+عملتهم لانفس الأورجانيزم ممنوع يكونوا اتنان
+
+255
+00:18:40,170 --> 00:18:44,190
+positive لازم يكون واحد لأن positive واحد negative
+
+256
+00:18:44,190 --> 00:18:47,850
+او اتنان negative لو كانت في الأصل ما بتعمل
+
+257
+00:18:47,850 --> 00:18:50,750
+Gelicose fermentation هذا ممنوع اني انا اشوفه لأن
+
+258
+00:18:50,750 --> 00:18:54,910
+البكتيريا اما بتسلك ال mixed acid pathway او ال
+
+259
+00:18:54,910 --> 00:18:59,910
+butylene glycol pathway ما بتسلك الاتنان مع بعض
+
+260
+00:19:03,410 --> 00:19:07,350
+يبقى لو جيبنا سؤال انه البريطس رياجانت used to
+
+261
+00:19:07,350 --> 00:19:11,670
+detect if the bacteria fermentation ferment in
+
+262
+00:19:11,670 --> 00:19:16,250
+acid pathway or not نحكي false البريطس اللي أنا
+
+263
+00:19:16,250 --> 00:19:21,130
+بستخدم عشان أشوف هل البكتيريا سلكت أو سلكت ال acid
+
+264
+00:19:21,130 --> 00:19:25,370
+pathway بستخدم اللي هو ال methyrid البريطس رياجانت
+
+265
+00:19:25,370 --> 00:19:31,190
+بستخدمه في البيوتانديول pathway يبقى زي ما قلنا ان
+
+266
+00:19:31,190 --> 00:19:35,300
+هذا ممنوع اني انا أشوفهالـ Media نفسها لفحص الـ
+
+267
+00:19:35,300 --> 00:19:38,280
+Methyrid و الـ Vascular لكن الاختلاف في الـ
+
+268
+00:19:38,280 --> 00:19:44,220
+Reagent الـ Reagent بيكون في الـ Mixed Acid
+
+269
+00:19:44,220 --> 00:19:47,560
+Pathway الـ Methyrid في البيوتين البيوتيولينيش
+
+270
+00:19:47,560 --> 00:19:47,860
+البيوتيولينيش البيوتيولينيش البيوتيولينيش
+
+271
+00:19:47,860 --> 00:19:48,440
+البيوتيولينيش البيوتيولينيش البيوتيولينيش
+
+272
+00:19:48,440 --> 00:19:48,660
+البيوتيولينيش البيوتيولينيش البيوتيولينيش
+
+273
+00:19:48,660 --> 00:19:50,420
+البيوتيولينيش البيوتيولينيش البيوتيولينيش
+
+274
+00:19:50,420 --> 00:19:52,220
+البيوتيولينيش البيوتيولينيش البيوتيولينيش
+
+275
+00:19:52,220 --> 00:19:57,860
+البيوتيولينيش البيوتيولينيش البيوتيولينيش
+
+276
+00:19:57,860 --> 00:20:00,820
+البيوتيولينيش البيوتيول
+
+277
+00:20:01,770 --> 00:20:05,550
+لو شوفت عملت الفحص وضفت الـ Parity reagent هتكون
+
+278
+00:20:05,550 --> 00:20:09,690
+positive للواقع سبسكوار لكن الفحص ال fermentation
+
+279
+00:20:09,690 --> 00:20:14,480
+قلنا النتيجة ال positive بتكون لون yellowهكذا نكون
+
+280
+00:20:14,480 --> 00:20:18,000
+خلصنا تقريبا أهم الأشياء اللي لازم نعرفها في فحص
+
+281
+00:20:18,000 --> 00:20:22,240
+الميثايريد فوكس بوسكواريه هنجي لسلايدات نمر عليها
+
+282
+00:20:22,240 --> 00:20:26,260
+بقول فحص الميثايريد some coliform will ferment
+
+283
+00:20:26,260 --> 00:20:30,200
+that extracts the dextrose اللي هو الجليكوز to
+
+284
+00:20:30,200 --> 00:20:33,720
+acid product that will cause the pH to drop below
+
+285
+00:20:33,720 --> 00:20:38,040
+pH 5 this is called a mixed acid fermentation قلنا
+
+286
+00:20:38,040 --> 00:20:41,280
+انه some coliform مش كل ال .. ال .. الcoliform
+
+287
+00:20:41,280 --> 00:20:45,080
+بتعمل fermentation لل dextrose لالـ Strong Acid
+
+288
+00:20:45,080 --> 00:20:49,480
+فقط الـ E.coli من الـ من الكوليفورم هي positive
+
+289
+00:20:49,480 --> 00:20:53,720
+لالميثايل Red بتسلق الـ Mixed Acid Fermentation
+
+290
+00:20:53,720 --> 00:20:57,800
+هذا هينتج Strong Acid هيعمل على لحفظ الـ pH بصورة
+
+291
+00:20:57,800 --> 00:21:03,760
+كبيرة high decrease in pH لأقل حتى من خمسة حتى أقل
+
+292
+00:21:03,760 --> 00:21:08,260
+هيكون من 4.4 بعد ال incubation بضيف مين بضيف ال
+
+293
+00:21:08,260 --> 00:21:11,700
+reaction للميثايل Red لفحص الـ Mixed Acid
+
+294
+00:21:11,700 --> 00:21:15,670
+Fermentationهذا الـ Methyl Red بقول هيتحول للون
+
+295
+00:21:15,670 --> 00:21:21,290
+أحمر في حال كان في عندي acid product هذا بيدل أنه
+
+296
+00:21:21,290 --> 00:21:24,690
+صار في عندي fermentation mixed acid fermentation
+
+297
+00:21:24,690 --> 00:21:29,810
+بنلاحظ أن اللون هانتحول للون أحمر بعد ما أنا أضفت
+
+298
+00:21:29,810 --> 00:21:33,130
+ال reaction ل Methyl Red يبقى البكتيريا كانت E
+
+299
+00:21:33,130 --> 00:21:37,250
+-coli ال positive لهذا الفحص Methyl Red E-coli ال
+
+300
+00:21:37,250 --> 00:21:40,610
+Antirobacter قلنا negative مافي بيصير أي تغير في
+
+301
+00:21:40,610 --> 00:21:44,020
+لون اللي نديها إذا ملاحظين highuninculated مش
+
+302
+00:21:44,020 --> 00:21:47,800
+مزروعهاك بتكون لون الميديا مافي اي تغير في لون
+
+303
+00:21:47,800 --> 00:21:50,980
+الميديا يبقى هي negative زي ال Eclipsilla و ال
+
+304
+00:21:50,980 --> 00:21:55,320
+Enterobacter Vagas-Boschauer Test و for other
+
+305
+00:21:55,320 --> 00:22:00,740
+coliform البكتيريا اللى ماعملت اللى هو ال mix acid
+
+306
+00:22:00,740 --> 00:22:05,580
+fermentation اكيد هتعمل ال Vagas-Boschauer Test او
+
+307
+00:22:05,580 --> 00:22:11,620
+ال butanediol pathwayهتحول الـ dextrose الجليكوز
+
+308
+00:22:11,620 --> 00:22:16,160
+to less acidic product لـ product أقل حمضية الكحول
+
+309
+00:22:16,160 --> 00:22:20,780
+product زي ال ethanol or butanediol هذه البكتيريا
+
+310
+00:22:20,780 --> 00:22:24,040
+بقولي بتكون negative في فحص الميثايريد لو أنا عملت
+
+311
+00:22:24,040 --> 00:22:27,220
+فحص الميثايريد هتكون negative في الميثايريد لكن
+
+312
+00:22:27,220 --> 00:22:31,300
+positive في الفاكسبوسكاالـ Butanediol fermentation
+
+313
+00:22:31,300 --> 00:22:35,560
+الـ pathway هذا قلناه أنا بدي أقدر أكشف عنه بالـ
+
+314
+00:22:35,560 --> 00:22:39,920
+Vagus Piscolar Test هيقصلي وجود الـ Acetone لأنه
+
+315
+00:22:39,920 --> 00:22:43,660
+احنا قلنا الـ Reagent VB1 بيرتبط بالـ Acetone مش
+
+316
+00:22:43,660 --> 00:22:47,240
+بالـ Butanediol يبقى هو هيكشف عن وجود الـ Acetone
+
+317
+00:22:47,240 --> 00:22:51,980
+اللي هو بيكون أساسي لـ Butanediol الـ Butanediol
+
+318
+00:22:51,980 --> 00:22:55,960
+الـ Acetone بيتحول لـ Butanediol الـ Butanediol هو
+
+319
+00:22:55,960 --> 00:22:58,120
+stable neutral
+
+320
+00:23:00,050 --> 00:23:04,910
+التي نان الفحصين الـ methyl red test و ال podcast
+
+321
+00:23:04,910 --> 00:23:07,690
+prescribed test بستخدم نفس ال media لأن ال
+
+322
+00:23:07,690 --> 00:23:11,430
+substrate نفسها هي الجليكوز لكن ال reagent بيختلف
+
+323
+00:23:11,430 --> 00:23:17,700
+بضيف بعض ال incubation بقولي أن أنا الفحصينبعملهم
+
+324
+00:23:17,700 --> 00:23:24,740
+بنفس الوقت عشان أشوف هل عشان أشوف يعني إذا هي أكيد
+
+325
+00:23:24,740 --> 00:23:28,640
+كانت positive لواحد هتكون negative للتانية عشان
+
+326
+00:23:28,640 --> 00:23:32,480
+أتأكد أنه واحدة positive والتانية لازم تكون
+
+327
+00:23:32,480 --> 00:23:37,460
+negative بارتس رياجونت البارتس رياجونت عشان أكشف
+
+328
+00:23:37,460 --> 00:23:42,920
+على ال focus buscler test قلنا ال VB1 5% αنفثالالـ
+
+329
+00:23:42,920 --> 00:23:47,460
+VB2 اللى هو 40% بوتاسيوم هيدروكسايد الـ KOH بضيفه
+
+330
+00:23:47,460 --> 00:23:54,480
+بعد 24 ساعة to 24 hour culture قولنا بعد ما تكون
+
+331
+00:23:54,480 --> 00:23:59,080
+المزرعة قعدت incubation 24 ساعة ممنوع اني احطها
+
+332
+00:23:59,080 --> 00:24:03,900
+بعد 72 ساعة الـ tube بعملها شاكن بحاول اني انا
+
+333
+00:24:03,900 --> 00:24:09,890
+اعمل شاكن عشان انه يصير mixبتحول اللون للون pink
+
+334
+00:24:09,890 --> 00:24:14,770
+او red بعد ما انا اعمل checking هذا بيدل على ال
+
+335
+00:24:14,770 --> 00:24:18,430
+positive reaction نتيجة وجود اللي هو ال Acetone
+
+336
+00:24:18,430 --> 00:24:22,410
+وليس Libyutanediol لإنه احنا قلنا ال reagent
+
+337
+00:24:22,410 --> 00:24:25,850
+بيرتبط مع ال Acetone وليس Libyutanediol عشان هيك
+
+338
+00:24:25,850 --> 00:24:32,490
+لازم ال culture تكون ما اتزيد عن 72 ساعة ملاحظها
+
+339
+00:24:32,490 --> 00:24:37,100
+لفوكاس بوسكوار بيكون لون هقولنا أصفرهذه ليست
+
+340
+00:24:37,100 --> 00:24:40,860
+مزروعة، هي نفسها لـ Methyl Arid الـ Positive،
+
+341
+00:24:40,860 --> 00:24:43,860
+نلاحظ أنه فيه أنجلون أحمر قلنا الـ Positive في
+
+342
+00:24:43,860 --> 00:24:46,420
+الفوكس بوسكوار هي الـ Enterobacter و الـ
+
+343
+00:24:46,420 --> 00:24:49,000
+Eclipsella اللي كانت Negative في الـ Methyl Arid
+
+344
+00:24:49,000 --> 00:24:51,120
+هي هذه الـ Eclipsella pneumonia والـ Enterobacter
+
+345
+00:24:51,120 --> 00:24:54,970
+Positive في فحص الفوكس بوسكوارلكن الـ E.coli و
+
+346
+00:24:54,970 --> 00:24:58,690
+البروتيز قلنا هيكونوا في فحص الفوكاسبوسكور نيجاتيف
+
+347
+00:24:58,690 --> 00:25:03,070
+ما تغير لون ال media هم لأنهم positive في
+
+348
+00:25:03,070 --> 00:25:06,210
+الميثايريد لأنهم بيعملوا mixed acid fermentation
+
+349
+00:25:06,210 --> 00:25:10,730
+فعلا في بعض الملاحظات اللي حكيناها برضه لو
+
+350
+00:25:10,730 --> 00:25:14,530
+البكتيريا ما بتعمل fermentation بالأصل للجليكوز لو
+
+351
+00:25:14,530 --> 00:25:17,790
+أنا عملت هذا الفحص الميثايريد و الفوكاسبوسكور
+
+352
+00:25:17,790 --> 00:25:22,930
+هتكون negative في الاتنين زي الصدومانسبقولّي ضروري
+
+353
+00:25:22,930 --> 00:25:28,190
+أنك انت تحطي الـreagent بشكل متسلسل لو ضفت الـKOH
+
+354
+00:25:28,190 --> 00:25:31,590
+اللي هو الـVB2 بالأول بقولّي هيتفاعل مع البيبتوني
+
+355
+00:25:31,590 --> 00:25:36,330
+الموجود في الـmedia هيعطيني Salmon pink color اللي
+
+356
+00:25:36,330 --> 00:25:40,390
+هو لون السمك السلمون فبالتالي أنا هفكره هو
+
+357
+00:25:40,390 --> 00:25:43,830
+الـpositive result لأنه الـpositive result بتكون
+
+358
+00:25:43,830 --> 00:25:48,790
+عندى لون أحمرلكن هذا false positive result KOH
+
+359
+00:25:48,790 --> 00:25:53,350
+اللي بيرتبط مع البيبتون لكن احنا قلنا ال reagent
+
+360
+00:25:53,350 --> 00:25:57,470
+ال VB1 بيرتبط مع الacetylene عشان يعطيني اللون
+
+361
+00:25:57,470 --> 00:26:01,470
+الأحمر VB test قلنا ال focus discover لازم تكون ال
+
+362
+00:26:01,470 --> 00:26:05,450
+culture ممنوع اني انا اعملها بعد 72 ساعة of
+
+363
+00:26:05,450 --> 00:26:11,630
+incubation لأنه هيعطيني weekly positive or false
+
+364
+00:26:11,630 --> 00:26:16,450
+negative result ليش لأنه احنا قلنا انهبعد 72 ساعة
+
+365
+00:26:16,450 --> 00:26:20,430
+الـ Acetone الـ Unstable بيتحول لمركّب
+
+366
+00:26:20,430 --> 00:26:24,150
+البيوتانديول الستابل وقلنا إن الـ Reagent الـ V.1
+
+367
+00:26:24,150 --> 00:26:28,110
+بيرتبط مع ال Acetone وليس مع البيوتانديول فلو أنا
+
+368
+00:26:28,110 --> 00:26:33,130
+حطيت بعد 72 ساعة هيكون اتحول لبيوتانديول هييجي
+
+369
+00:26:33,130 --> 00:26:36,370
+يدور على ال Acetone مش هيلاقيه فبالتالي مش هيرتبط
+
+370
+00:26:36,370 --> 00:26:40,870
+معاه ما هيعطيني اللون ال Red فهفكر أنا النتيجة هي
+
+371
+00:26:40,870 --> 00:26:44,640
+Negative لكن هي False Negative Resultلأني أنا
+
+372
+00:26:44,640 --> 00:26:48,900
+استخدمت مزرعة بعد 72 ساعة من الـ incubation حيث
+
+373
+00:26:48,900 --> 00:26:52,940
+بنكون خلصنا فحص الـ Methyl Red Vox Biscuit Test
+
+374
+00:26:52,940 --> 00:26:57,940
+هذا الفحص بعمله فقط لو كانت البكتيريا بالاصل كانت
+
+375
+00:26:57,940 --> 00:27:01,540
+fermentation جليكوز fermenter بتعمل fermentation
+
+376
+00:27:01,540 --> 00:27:09,860
+لجليكوز هذا الفحص برضه بستخدمه لتفريق في الـ
+
+377
+00:27:09,860 --> 00:27:16,140
+coliform بكتيريا في أول slide نسينانمر عليها
+
+378
+00:27:16,140 --> 00:27:19,940
+الميثايردفوكاس بوسكوهر إبقاش significant لهذا
+
+379
+00:27:19,940 --> 00:27:22,560
+الفحص used to differentiate between enteric
+
+380
+00:27:22,560 --> 00:27:25,640
+bacilli اللي هي الكوليفارم اللي قلنا انتروباكتر
+
+381
+00:27:25,640 --> 00:27:30,360
+ستروباكتر إيكولايكليبسيلا مع الاندول والسايتريت
+
+382
+00:27:30,360 --> 00:27:33,840
+السايتريت قلنا باستخدمه عشان أفرق الكوليفارم
+
+383
+00:27:33,840 --> 00:27:37,340
+بكتيريا كانت ال إيكولاي نيجاتيف والباقي positive
+
+384
+00:27:38,030 --> 00:27:41,830
+بستخدم برضه فحص الـ Methyrid Focus Biscuer و ال
+
+385
+00:27:41,830 --> 00:27:44,890
+Endol هدول الأربع فحصات عشان أفرق الـ Coliform
+
+386
+00:27:44,890 --> 00:27:49,110
+بكتيريا some of these pathway produce unstable
+
+387
+00:27:49,110 --> 00:27:52,790
+acidic product بقولي بعض أنواع البكتيريا بتسلق
+
+388
+00:27:52,790 --> 00:27:58,350
+سلوك اللي هو الـ butanediol pathway بتنتج unstable
+
+389
+00:27:58,350 --> 00:28:02,180
+acidic productwhich quickly convert to neutral
+
+390
+00:28:02,180 --> 00:28:05,240
+compound هتتحول ل neutral compound عشان هيك انا
+
+391
+00:28:05,240 --> 00:28:08,640
+سميت ال pathway ب neutral compound some organism
+
+392
+00:28:08,640 --> 00:28:13,120
+use the butylene glycol pathway هاي البكتيريا
+
+393
+00:28:13,120 --> 00:28:17,200
+بتستخدم ال butylene glycol pathway اللي بتنتج
+
+394
+00:28:17,200 --> 00:28:20,920
+الكحول which produce neutral end product بتكون
+
+395
+00:28:20,920 --> 00:28:24,960
+neutral end product including acetylene and 2,3
+
+396
+00:28:24,960 --> 00:28:29,840
+-butanediolنواتج عملية الـ fermentation الكحول
+
+397
+00:28:29,840 --> 00:28:34,520
+الاسيتوين أونستابللكن البيوتانديول هو الستابل
+
+398
+00:28:34,520 --> 00:28:38,760
+other organism ال organism الآخر قولنا انه بتسلق
+
+399
+00:28:38,760 --> 00:28:42,160
+ال mixed acid pathway هتنتج acid in the product
+
+400
+00:28:42,160 --> 00:28:45,740
+قولنا strong acid زي ال lactic acid ال acidic acid
+
+401
+00:28:45,740 --> 00:28:49,260
+and formic acid هاي ال acidic in the product قولنا
+
+402
+00:28:49,260 --> 00:28:53,960
+هي stable على عكس هي كانت unstable و بتظلها acidic
+
+403
+00:28:53,960 --> 00:28:57,980
+strong acidic هتعمل على ال high decrease in pH لكن
+
+404
+00:28:57,980 --> 00:29:02,160
+هانهتعمل slide decrease للـ pH فعشان هيك أنا بضيف
+
+405
+00:29:02,160 --> 00:29:06,740
+reaction عشان يرتبط مع النواتج وخاصة الـ Acetone
+
+406
+00:29:06,740 --> 00:29:10,960
+يعني ال VB1 بيرتبط بالـ Acetone هيك بنكون خلصنا
+
+407
+00:29:10,960 --> 00:29:15,320
+الفحص الميثايريد فوكاس بوسكوار وخلصنا فحص ال
+
+408
+00:29:15,320 --> 00:29:19,440
+fermentation البكتيريا ال fermentation وميثايريد
+
+409
+00:29:19,440 --> 00:29:22,160
+فوكاس بوسكوار قلنا الميثايريد فوكاس بوسكوار إيه له
+
+410
+00:29:22,160 --> 00:29:25,420
+علاقة في ال fermentation عشان هيك لازم يكونوا
+
+411
+00:29:25,420 --> 00:29:30,390
+اتنين مع بعضيعطيكم العافية جميعًا والسلام عليكم
+
+412
+00:29:30,390 --> 00:29:31,770
+ورحمة الله وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/fiNRLFNYLh8_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/fiNRLFNYLh8_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..96eb7447e069d8f9447bbdccd474219e63adeebd
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/fiNRLFNYLh8_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 2923, "start": 2.33, "end": 29.23, "text": "Methyl redvocas-buscowar test هذا الفحص له علاقة بالبكتيريا ال fermentation لو البكتيريا عملت fermentation للجليكوز هعمل فحص الـ Methyl redvocas-buscowar قولنا إن من نتائج عملية ال fermentation ال end product إما يكون أسد أو الكحول وقلنا إن ال end product هي من خلالها أنا بقدر أعمل identification للبكتيريا", "tokens": [44, 3293, 5088, 2182, 20836, 296, 12, 21441, 66, 305, 289, 1500, 23758, 27188, 5016, 9381, 46740, 11203, 995, 28671, 20666, 3555, 4117, 2655, 13546, 25528, 2423, 43161, 45164, 29739, 4117, 2655, 13546, 25528, 6225, 42213, 2655, 43161, 24976, 7435, 20292, 4117, 2407, 11622, 8032, 25957, 1211, 6156, 5016, 9381, 2423, 39184, 48602, 5088, 2182, 20836, 296, 12, 21441, 66, 305, 289, 12174, 12610, 8315, 36145, 9154, 8717, 2655, 16373, 7435, 6225, 42213, 10632, 2423, 43161, 2423, 917, 1674, 11933, 15042, 7251, 30544, 5551, 3794, 3215, 34051, 33251, 5016, 12610, 4032, 4587, 1211, 8315, 36145, 2423, 917, 1674, 39896, 9154, 16490, 1211, 6027, 11296, 41850, 4724, 28543, 2288, 5551, 25957, 1211, 22065, 24976, 3555, 4117, 2655, 13546, 25528], "avg_logprob": -0.2183560981970875, "compression_ratio": 1.8319672131147542, "no_speech_prob": 2.3245811462402344e-06, "words": [{"start": 2.33, "end": 3.03, "word": "Methyl", "probability": 0.73779296875}, {"start": 3.03, "end": 3.93, "word": " redvocas", "probability": 0.3414306640625}, {"start": 3.93, "end": 4.55, "word": "-buscowar", "probability": 0.519140625}, {"start": 4.55, "end": 4.87, "word": " test", "probability": 0.75439453125}, {"start": 4.87, "end": 5.17, "word": " هذا", "probability": 0.476318359375}, {"start": 5.17, "end": 5.83, "word": " الفحص", "probability": 0.9558919270833334}, {"start": 5.83, "end": 6.03, "word": " له", "probability": 0.556640625}, {"start": 6.03, "end": 6.61, "word": " علاقة", "probability": 0.982421875}, {"start": 6.61, "end": 7.73, "word": " بالبكتيريا", "probability": 0.899169921875}, {"start": 7.73, "end": 7.87, "word": " ال", "probability": 0.64599609375}, {"start": 7.87, "end": 8.43, "word": " fermentation", "probability": 0.65234375}, {"start": 8.43, "end": 9.03, "word": " لو", "probability": 0.8369140625}, {"start": 9.03, "end": 9.59, "word": " البكتيريا", "probability": 0.9724609375}, {"start": 9.59, "end": 9.99, "word": " عملت", "probability": 0.9886067708333334}, {"start": 9.99, "end": 10.55, "word": " fermentation", "probability": 0.98486328125}, {"start": 10.55, "end": 11.93, "word": " للجليكوز", "probability": 0.7322591145833334}, {"start": 11.93, "end": 12.69, "word": " هعمل", "probability": 0.9508463541666666}, {"start": 12.69, "end": 13.03, "word": " فحص", "probability": 0.9931640625}, {"start": 13.03, "end": 13.31, "word": " الـ", "probability": 0.500244140625}, {"start": 13.31, "end": 13.75, "word": " Methyl", "probability": 0.7183837890625}, {"start": 13.75, "end": 14.71, "word": " redvocas", "probability": 0.8837890625}, {"start": 14.71, "end": 15.49, "word": "-buscowar", "probability": 0.953125}, {"start": 15.49, "end": 16.39, "word": " قولنا", "probability": 0.8546549479166666}, {"start": 16.39, "end": 16.61, "word": " إن", "probability": 0.64697265625}, {"start": 16.61, "end": 16.77, "word": " من", "probability": 0.6025390625}, {"start": 16.77, "end": 17.43, "word": " نتائج", "probability": 0.9906005859375}, {"start": 17.43, "end": 18.07, "word": " عملية", "probability": 0.9890950520833334}, {"start": 18.07, "end": 18.39, "word": " ال", "probability": 0.9755859375}, {"start": 18.39, "end": 18.95, "word": " fermentation", "probability": 0.94140625}, {"start": 18.95, "end": 19.39, "word": " ال", "probability": 0.8291015625}, {"start": 19.39, "end": 19.69, "word": " end", "probability": 0.5068359375}, {"start": 19.69, "end": 20.17, "word": " product", "probability": 0.93701171875}, {"start": 20.17, "end": 20.43, "word": " إما", "probability": 0.88330078125}, {"start": 20.43, "end": 20.83, "word": " يكون", "probability": 0.992919921875}, {"start": 20.83, "end": 21.41, "word": " أسد", "probability": 0.7308756510416666}, {"start": 21.41, "end": 21.79, "word": " أو", "probability": 0.98974609375}, {"start": 21.79, "end": 22.91, "word": " الكحول", "probability": 0.7557779947916666}, {"start": 22.91, "end": 23.67, "word": " وقلنا", "probability": 0.7186279296875}, {"start": 23.67, "end": 23.89, "word": " إن", "probability": 0.88720703125}, {"start": 23.89, "end": 24.21, "word": " ال", "probability": 0.96533203125}, {"start": 24.21, "end": 24.47, "word": " end", "probability": 0.77587890625}, {"start": 24.47, "end": 24.91, "word": " product", "probability": 0.9521484375}, {"start": 24.91, "end": 25.31, "word": " هي", "probability": 0.67529296875}, {"start": 25.31, "end": 25.47, "word": " من", "probability": 0.92919921875}, {"start": 25.47, "end": 26.03, "word": " خلالها", "probability": 0.9932861328125}, {"start": 26.03, "end": 26.27, "word": " أنا", "probability": 0.86474609375}, {"start": 26.27, "end": 26.79, "word": " 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end", "probability": 0.173095703125}, {"start": 33.33, "end": 33.89, "word": " product", "probability": 0.90185546875}, {"start": 33.89, "end": 34.29, "word": " من", "probability": 0.927734375}, {"start": 34.29, "end": 34.63, "word": " خلال", "probability": 0.9933268229166666}, {"start": 34.63, "end": 34.97, "word": " فحص", "probability": 0.9602864583333334}, {"start": 34.97, "end": 35.15, "word": " الـ", "probability": 0.67041015625}, {"start": 35.15, "end": 35.67, "word": " methyl", "probability": 0.42529296875}, {"start": 35.67, "end": 36.13, "word": " red", "probability": 0.86767578125}, {"start": 36.13, "end": 36.63, "word": " vocas", "probability": 0.2734375}, {"start": 36.63, "end": 37.19, "word": " buscala", "probability": 0.3877766927083333}, {"start": 37.19, "end": 37.85, "word": " عشان", "probability": 0.95068359375}, {"start": 37.85, "end": 38.47, "word": " نعرف", "probability": 0.9923502604166666}, {"start": 38.47, "end": 39.31, "word": " أكتر", "probability": 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"probability": 0.979248046875}, {"start": 674.5, "end": 674.92, "word": " رياجون", "probability": 0.969482421875}, {"start": 674.92, "end": 675.3, "word": " بعد", "probability": 0.9716796875}, {"start": 675.3, "end": 675.9, "word": " ال", "probability": 0.98681640625}, {"start": 675.9, "end": 676.64, "word": " incubation", "probability": 0.907958984375}, {"start": 676.64, "end": 677.34, "word": " وقوللي", "probability": 0.5831705729166666}, {"start": 677.34, "end": 678.04, "word": " بتضيفي", "probability": 0.89208984375}, {"start": 678.04, "end": 678.66, "word": " VB1", "probability": 0.8203125}, {"start": 678.66, "end": 679.16, "word": " بعدين", "probability": 0.769775390625}, {"start": 679.16, "end": 679.74, "word": " بتضيفي", "probability": 0.8857421875}, {"start": 679.74, "end": 680.36, "word": " VB2", "probability": 0.9842122395833334}], "temperature": 1.0}, {"id": 27, "seek": 70858, "start": 680.7, "end": 708.58, "text": "ممنوع أنا أبدل هنحكي السبب بعد ما نشرح كيف أو إيهش التفاعل 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false negative result عشان هيك لازم اني", "tokens": [1211, 39184, 583, 282, 10323, 401, 745, 712, 6156, 3555, 6027, 2655, 6027, 1829, 39182, 18731, 8608, 995, 27884, 4724, 1829, 30544, 2423, 39184, 5097, 302, 546, 7251, 2655, 5016, 12610, 5296, 39184, 583, 282, 10323, 401, 45164, 41850, 48812, 5172, 2655, 2423, 16572, 26108, 7649, 2655, 2423, 16572, 26108, 7649, 2655, 12174, 1211, 8315, 4724, 13546, 2655, 3555, 9566, 6156, 47432, 20449, 2423, 39184, 5097, 302, 546, 6156, 3555, 6027, 2655, 6027, 1829, 37893, 39896, 15040, 38436, 2423, 39184, 5097, 302, 546, 5296, 33456, 3224, 1975, 2655, 5016, 12610, 5296, 39184, 583, 282, 10323, 401, 6156, 3224, 1829, 3615, 9566, 9957, 1829, 3671, 1874, 37893, 39896, 2655, 5016, 12610, 13672, 11536, 5296, 1211, 11536, 4477, 39896, 30544, 406, 3105, 8978, 13672, 11536, 9673, 1829, 16254, 995, 6156, 3555, 6027, 2655, 6027, 1829, 39896, 3615, 9566, 1829, 7908, 3671, 1874, 6225, 8592, 7649, 39896, 4117, 5296, 31377, 2304, 16472, 1829], "avg_logprob": 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"probability": 0.8766276041666666}, {"start": 1731.08, "end": 1731.28, "word": " هي", "probability": 0.5380859375}, {"start": 1731.28, "end": 1731.66, "word": " كانت", "probability": 0.96142578125}, {"start": 1731.66, "end": 1732.26, "word": " unstable", "probability": 0.70263671875}, {"start": 1732.26, "end": 1732.84, "word": " و", "probability": 0.5791015625}, {"start": 1732.84, "end": 1733.52, "word": " بتظلها", "probability": 0.708740234375}, {"start": 1733.52, "end": 1733.96, "word": " acidic", "probability": 0.8203125}, {"start": 1733.96, "end": 1734.46, "word": " strong", "probability": 0.51416015625}, {"start": 1734.46, "end": 1734.88, "word": " acidic", "probability": 0.92919921875}, {"start": 1734.88, "end": 1735.52, "word": " هتعمل", "probability": 0.985595703125}, {"start": 1735.52, "end": 1735.76, "word": " على", "probability": 0.94482421875}, {"start": 1735.76, "end": 1736.04, "word": " ال", "probability": 0.9453125}, {"start": 1736.04, "end": 1736.5, "word": " high", "probability": 0.91162109375}, {"start": 1736.5, "end": 1737.0, "word": " decrease", "probability": 0.86328125}, {"start": 1737.0, "end": 1737.2, "word": " in", "probability": 0.7431640625}, {"start": 1737.2, "end": 1737.58, "word": " pH", "probability": 0.9443359375}, {"start": 1737.58, "end": 1737.98, "word": " لكن", "probability": 0.8828125}, {"start": 1737.98, "end": 1738.48, "word": " هان", "probability": 0.661376953125}], "temperature": 1.0}, {"id": 69, "seek": 176706, "start": 1738.78, "end": 1767.06, "text": "هتعمل slide decrease للـ pH فعشان هيك أنا بضيف reaction عشان يرتبط مع النواتج وخاصة الـ Acetone يعني ال VB1 بيرتبط بالـ Acetone هيك بنكون خلصنا الفحص الميثايريد فوكاس بوسكوار وخلصنا فحص ال fermentation البكتيريا ال fermentation وميثايريد فوكاس بوسكوار قلنا الميثايريد فوكاس بوسكوار إيه له علاقة في ال fermentation عشان هيك لازم يكونوا اتنين مع بعض", "tokens": [3224, 2655, 25957, 1211, 4137, 11514, 24976, 39184, 21677, 6156, 3615, 8592, 7649, 39896, 4117, 41850, 4724, 11242, 33911, 5480, 6225, 8592, 7649, 7251, 43500, 3555, 9566, 20449, 28239, 2407, 9307, 7435, 4032, 9778, 33546, 3660, 2423, 39184, 5097, 302, 546, 37495, 22653, 2423, 691, 33, 16, 4724, 13546, 2655, 3555, 9566, 20666, 39184, 5097, 302, 546, 39896, 4117, 44945, 30544, 16490, 1211, 9381, 8315, 27188, 5016, 9381, 9673, 1829, 12984, 995, 13546, 25708, 6156, 2407, 4117, 32277, 4724, 41779, 4117, 2407, 9640, 4032, 9778, 1211, 9381, 8315, 6156, 5016, 9381, 2423, 43161, 29739, 4117, 2655, 13546, 25528, 2423, 43161, 4032, 2304, 1829, 12984, 995, 13546, 25708, 6156, 2407, 4117, 32277, 4724, 41779, 4117, 2407, 9640, 12174, 1211, 8315, 9673, 1829, 12984, 995, 13546, 25708, 6156, 2407, 4117, 32277, 4724, 41779, 4117, 2407, 9640, 11933, 1829, 3224, 46740, 11203, 995, 28671, 8978, 2423, 43161, 6225, 8592, 7649, 39896, 4117, 5296, 31377, 2304, 7251, 30544, 14407, 1975, 2655, 1863, 9957, 20449, 45030, 11242], "avg_logprob": -0.1766679422255674, "compression_ratio": 2.051094890510949, "no_speech_prob": 2.4318695068359375e-05, "words": [{"start": 1738.78, "end": 1739.46, "word": "هتعمل", "probability": 0.9305419921875}, {"start": 1739.46, "end": 1739.92, "word": " slide", "probability": 0.521484375}, {"start": 1739.92, "end": 1740.4, "word": " decrease", "probability": 0.7978515625}, {"start": 1740.4, "end": 1740.7, "word": " للـ", "probability": 0.66064453125}, {"start": 1740.7, "end": 1741.06, "word": " pH", "probability": 0.68408203125}, {"start": 1741.06, "end": 1741.48, "word": " فعشان", "probability": 0.8709716796875}, {"start": 1741.48, "end": 1741.66, "word": " هيك", "probability": 0.807373046875}, {"start": 1741.66, "end": 1741.82, "word": " أنا", "probability": 0.7607421875}, {"start": 1741.82, "end": 1742.16, "word": " بضيف", "probability": 0.9440104166666666}, {"start": 1742.16, "end": 1742.48, "word": " reaction", "probability": 0.59130859375}, {"start": 1742.48, "end": 1742.94, "word": " عشان", "probability": 0.9840494791666666}, {"start": 1742.94, "end": 1743.44, "word": " يرتبط", "probability": 0.99267578125}, {"start": 1743.44, "end": 1743.7, "word": " مع", "probability": 0.9931640625}, {"start": 1743.7, "end": 1745.36, "word": " النواتج", "probability": 0.7132568359375}, {"start": 1745.36, "end": 1745.96, "word": " وخاصة", "probability": 0.91259765625}, {"start": 1745.96, "end": 1746.22, "word": " الـ", "probability": 0.57440185546875}, {"start": 1746.22, "end": 1746.74, "word": " Acetone", "probability": 0.74462890625}, {"start": 1746.74, "end": 1747.0, "word": " يعني", "probability": 0.7252197265625}, {"start": 1747.0, "end": 1747.16, "word": " ال", "probability": 0.8271484375}, {"start": 1747.16, "end": 1747.68, "word": " VB1", "probability": 0.5219319661458334}, {"start": 1747.68, "end": 1748.26, "word": " بيرتبط", "probability": 0.93984375}, {"start": 1748.26, "end": 1748.64, "word": " بالـ", "probability": 0.608154296875}, {"start": 1748.64, "end": 1749.32, "word": " Acetone", "probability": 0.94091796875}, {"start": 1749.32, "end": 1749.92, "word": " هيك", "probability": 0.736083984375}, {"start": 1749.92, "end": 1750.22, "word": " بنكون", "probability": 0.932373046875}, {"start": 1750.22, "end": 1750.96, "word": " خلصنا", "probability": 0.9847412109375}, {"start": 1750.96, "end": 1752.54, "word": " الفحص", "probability": 0.8458658854166666}, {"start": 1752.54, "end": 1753.46, "word": " الميثايريد", "probability": 0.6208597819010416}, {"start": 1753.46, "end": 1753.84, "word": " فوكاس", "probability": 0.8287353515625}, {"start": 1753.84, "end": 1754.3, "word": " بوسكوار", "probability": 0.83232421875}, {"start": 1754.3, "end": 1754.8, "word": " وخلصنا", "probability": 0.9494140625}, {"start": 1754.8, "end": 1755.16, "word": " فحص", "probability": 0.95751953125}, {"start": 1755.16, "end": 1755.32, "word": " ال", "probability": 0.94091796875}, {"start": 1755.32, "end": 1755.82, "word": " fermentation", "probability": 0.82275390625}, {"start": 1755.82, "end": 1757.62, "word": " البكتيريا", "probability": 0.826904296875}, {"start": 1757.62, "end": 1757.72, "word": " ال", "probability": 0.73095703125}, {"start": 1757.72, "end": 1758.18, "word": " fermentation", "probability": 0.87060546875}, {"start": 1758.18, "end": 1759.44, "word": " وميثايريد", "probability": 0.9127371651785714}, {"start": 1759.44, "end": 1759.78, "word": " فوكاس", "probability": 0.980224609375}, {"start": 1759.78, "end": 1760.24, "word": " بوسكوار", "probability": 0.9671875}, {"start": 1760.24, "end": 1760.6, "word": " قلنا", "probability": 0.740234375}, {"start": 1760.6, "end": 1761.2, "word": " الميثايريد", "probability": 0.911865234375}, {"start": 1761.2, "end": 1761.52, "word": " فوكاس", "probability": 0.985107421875}, {"start": 1761.52, "end": 1761.9, "word": " بوسكوار", "probability": 0.977734375}, {"start": 1761.9, "end": 1762.1, "word": " إيه", "probability": 0.7875162760416666}, {"start": 1762.1, "end": 1762.16, "word": " له", "probability": 0.84423828125}, {"start": 1762.16, "end": 1762.6, "word": " علاقة", "probability": 0.9853515625}, {"start": 1762.6, "end": 1763.28, "word": " في", "probability": 0.9228515625}, {"start": 1763.28, "end": 1763.5, "word": " ال", "probability": 0.96533203125}, {"start": 1763.5, "end": 1763.92, "word": " fermentation", "probability": 0.951171875}, {"start": 1763.92, "end": 1764.38, "word": " عشان", "probability": 0.9778645833333334}, {"start": 1764.38, "end": 1764.58, "word": " هيك", "probability": 0.9853515625}, {"start": 1764.58, "end": 1764.84, "word": " لازم", "probability": 0.9825846354166666}, {"start": 1764.84, "end": 1765.42, "word": " يكونوا", "probability": 0.9814453125}, {"start": 1765.42, "end": 1766.44, "word": " اتنين", "probability": 0.845703125}, {"start": 1766.44, "end": 1766.72, "word": " مع", "probability": 0.99560546875}, {"start": 1766.72, "end": 1767.06, "word": " بعض", "probability": 0.9951171875}], "temperature": 1.0}, {"id": 70, "seek": 177177, "start": 1767.69, "end": 1771.77, "text": "يعطيكم العافية جميعًا والسلام عليكم ورحمة الله وبركاته", "tokens": [40228, 9566, 1829, 24793, 18863, 31845, 10632, 10874, 2304, 40228, 14111, 995, 16070, 3794, 37440, 25894, 24793, 4032, 2288, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224], "avg_logprob": -0.06291852385870048, "compression_ratio": 1.2469135802469136, "no_speech_prob": 5.364418029785156e-07, "words": [{"start": 1767.69, "end": 1768.45, "word": "يعطيكم", "probability": 0.96435546875}, {"start": 1768.45, "end": 1768.87, "word": " العافية", "probability": 0.9654947916666666}, {"start": 1768.87, "end": 1769.47, "word": " جميعًا", "probability": 0.94794921875}, {"start": 1769.47, "end": 1769.95, "word": " والسلام", "probability": 0.8126627604166666}, {"start": 1769.95, "end": 1770.39, "word": " عليكم", "probability": 0.952392578125}, {"start": 1770.39, "end": 1770.85, "word": " ورحمة", "probability": 0.98388671875}, {"start": 1770.85, "end": 1771.11, "word": " الله", "probability": 0.9716796875}, {"start": 1771.11, "end": 1771.77, "word": " وبركاته", "probability": 0.9697265625}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 1772.7274375, "duration_after_vad": 1740.2668749999953}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/hwINTbSvNaQ.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/hwINTbSvNaQ.srt
new file mode 100644
index 0000000000000000000000000000000000000000..0d30e7d4c01143f5ae2482bbf4f21975e6fd6d2d
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/hwINTbSvNaQ.srt
@@ -0,0 +1,307 @@
+1
+00:00:00,670 --> 00:00:04,490
+في الأسبوع السابع والثامن والتاسع والعاشر هذه
+
+2
+00:00:04,490 --> 00:00:08,670
+الأسبوع كلها هناخد الـ biochemical test الفحوصات
+
+3
+00:00:08,670 --> 00:00:13,250
+الكيميائية. أنا عشان أوصل لتشخيص الصحيح لازم أجري هذه
+
+4
+00:00:13,250 --> 00:00:17,320
+الفحوصات. قبل ما أجري أي فحص لازم يكون فيه اندي أنا
+
+5
+00:00:17,320 --> 00:00:21,300
+pure culture. طبعا الـ pure culture، طبعا احنا قلنا
+
+6
+00:00:21,300 --> 00:00:26,220
+أن كل المستعمرة على الطبق ماخدة نفس الشكل، أي أن
+
+7
+00:00:26,220 --> 00:00:31,280
+أصل الكولوني هو خلية بكتيرية واحدة. سنتعرف على كل
+
+8
+00:00:31,280 --> 00:00:36,500
+فحص بالتفصيل. سنتعرف على الـ principle  الفحص، سنتعرف
+
+9
+00:00:36,500 --> 00:00:40,920
+على أهمية كل فحص، كيف نعمل الفحص،
+
+10
+00:00:40,920 --> 00:00:43,840
+شو النتيجة الـ Positive، كيف بتكون، شو النتيجة الـ
+
+11
+00:00:43,840 --> 00:00:47,500
+Negative، كيف بتكون شكلها، من الـ Positive لهذا
+
+12
+00:00:47,500 --> 00:00:51,120
+الفحص من البكتيريا، ومن الـ Negative لكل فحص من
+
+13
+00:00:51,120 --> 00:00:55,180
+البكتيريا. بالنسبة للأسبوع الـ 11، في الأسبوع الـ
+
+14
+00:00:55,180 --> 00:00:58,980
+11 سنتعرف على الـ Bacterial Oxygen Requirement and
+
+15
+00:00:58,980 --> 00:01:02,640
+Anaerobic Bacteria. طبعا البكتيريا مصنفة حسب
+
+16
+00:01:02,640 --> 00:01:06,620
+حاجتها للأكسجين: بكتيريا هوائية، بكتيريا لا هوائية،
+
+17
+00:01:06,620 --> 00:01:10,820
+بكتيريا تحتاج الـCO2 مثلا. فبالتالي أنا لما بدي
+
+18
+00:01:10,820 --> 00:01:14,160
+أعزل بكتيريا X وأنا عارفة أن بكتيريا X تحتاج
+
+19
+00:01:14,160 --> 00:01:17,900
+الـCO2، فلازم أوفر ظروف الـCO2 حتى تنمو. لو أنا
+
+20
+00:01:17,900 --> 00:01:21,240
+ما وفرت هذه الظروف البكتيريا مش هتنمو. فهذا المعمل
+
+21
+00:01:21,240 --> 00:01:26,040
+سنّتعرف كيف نوفر مثلا ظروف هوائية، كيف نوفر ظروف لا
+
+22
+00:01:26,040 --> 00:01:30,070
+هوائية، كيف أنا بدي أوفر ظروف CO2. في الأسبوع الـ 12
+
+23
+00:01:30,070 --> 00:01:34,810
+سنّتعرف على single media multiple test. طبعا هذا يتبع للـ
+
+24
+00:01:34,810 --> 00:01:38,890
+biochemical test. أنا في الـ biochemical test لو بدي
+
+25
+00:01:38,890 --> 00:01:43,350
+أعمل مثلا، عشان أوصل لتشخيص صحيح، أكيد هعمل أكثر من
+
+26
+00:01:43,350 --> 00:01:47,010
+فحص كيميائي، لأن كل ما زادت عدد الفحوصات كل ما زادت
+
+27
+00:01:47,010 --> 00:01:52,610
+الدقة في التشخيص. تخيلوا أنا بدي أشخص بكتيريا معينة
+
+28
+00:01:52,610 --> 00:01:57,850
+وبدي أعمل كذا فحص، بالتالي هياخد مني وقت و
+
+29
+00:01:57,850 --> 00:02:02,290
+هياخد مني مجهود. أن كل فحص بده أدوات خاصة، بدي
+
+30
+00:02:02,290 --> 00:02:07,250
+أحضر لكل فحص مثلا media خاصة. هذا هياخد مني وقت و
+
+31
+00:02:07,250 --> 00:02:10,870
+جهد. فكرة الـ Single Media Multiple Test أنه ميديا
+
+32
+00:02:10,870 --> 00:02:15,670
+واحدة، أزرع البكتيريا اللي أنا بدي طبعا أعرف أو بدي
+
+33
+00:02:15,670 --> 00:02:20,030
+أشخصها، وأعرف هويتها. وثاني يوم باجي بشوف النتائج
+
+34
+00:02:20,030 --> 00:02:25,850
+بكون طبعا النتيجة بعد 24 ساعة. بقرأ نتيجة طبعا أكثر
+
+35
+00:02:25,850 --> 00:02:30,090
+من فحص في نفس الميديا. في الأسبوع الثالث عشر، فيه شيء
+
+36
+00:02:30,090 --> 00:02:33,030
+اسمه، هناخد معملين. طبعا Selective and Differential
+
+37
+00:02:33,030 --> 00:02:36,390
+Media. وفي معمل ثاني منفصل عنه اللي هو Serial
+
+38
+00:02:36,390 --> 00:02:39,870
+Dilution. بالنسبة لـ Selective and Differential
+
+39
+00:02:39,870 --> 00:02:42,990
+Media، قلنا ميديا هو وسط غذائي. الوسط الغذائي فيه
+
+40
+00:02:42,990 --> 00:02:45,570
+عندنا، إيه اللي هو تصنيفات مختلفة. سنتعرف في هذا
+
+41
+00:02:45,570 --> 00:02:49,070
+المعمل على هذه التصنيفات. فيه من ضمن التصنيفات شيء
+
+42
+00:02:49,070 --> 00:02:52,450
+اسمه اللي هو الـ Selective Media. الـ Selective
+
+43
+00:02:52,450 --> 00:02:58,840
+معناها اللي هي انتقائي أو اختياري. يعني أنا بدي أنمي
+
+44
+00:02:58,840 --> 00:03:03,480
+مثلا الـ bacteria اللي هي الـ Mojapetgram. في عندي
+
+45
+00:03:03,480 --> 00:03:07,960
+ميديا خاصة بتنمي فقط الـ Mojapetgram وبتثبط الـ
+
+46
+00:03:07,960 --> 00:03:11,320
+Celebitgram. طبعا أكيد في الميديا في مكون بيعمل على
+
+47
+00:03:11,320 --> 00:03:16,540
+تثبيط مثلا الـ Celebitgram. سنتعرف على أنواع متعددة
+
+48
+00:03:16,540 --> 00:03:19,760
+من الـ Selective Media. الـ Differential Media أن
+
+49
+00:03:19,760 --> 00:03:24,440
+هي بتنمي أكثر منها، لكن بتعطي مثلا بكتيريا X لون على
+
+50
+00:03:24,440 --> 00:03:29,440
+هذا الوسط، وبتعطي بكتيريا Y لون ثاني على نفس الوسط.
+
+51
+00:03:29,440 --> 00:03:33,140
+يمكن شفتوا في صورة الـ group الـ WhatsApp فيه
+
+52
+00:03:33,140 --> 00:03:38,500
+أندي. طبق مقسم نصين، نصه مزروع عليه بكتيريا X هو
+
+53
+00:03:38,500 --> 00:03:43,770
+النص الثاني مزروع عليه بكتيريا Y. X أعطت لون أصفر، وY
+
+54
+00:03:43,770 --> 00:03:46,590
+أعطت لون colorless. فهذه تعتبر الـ differential
+
+55
+00:03:46,590 --> 00:03:50,570
+media. الـ Serial dilution، طبعا احنا قلنا أن
+
+56
+00:03:50,570 --> 00:03:56,750
+البكتيريا ما بشوفها إلا على شكل مستعمرات
+
+57
+00:03:56,750 --> 00:04:01,350
+فبالتالي أنا لو بدي أعد البكتيريا مش هقدر أعد
+
+58
+00:04:01,350 --> 00:04:05,370
+البكتيريا، لأن الكولوني هو تجمع ملايين من الخلايا
+
+59
+00:04:05,370 --> 00:04:08,910
+البكتيرية. في هذا المعمل سنتعرف كيف أنا لو بدي أعد
+
+60
+00:04:08,910 --> 00:04:12,510
+البكتيريا، مثلا بدي أشوف مدى استجابة المريض للـ
+
+61
+00:04:12,510 --> 00:04:16,170
+Antibiotic، هل قلّ أو زاد. هنعرف من خلال الـ Serial
+
+62
+00:04:16,170 --> 00:04:20,590
+Dilution. في الأسبوع الـ 14 سنتعرف على Growth Curve
+
+63
+00:04:20,590 --> 00:04:24,290
+and Microbial Control Agents. سنتعرف على نمو
+
+64
+00:04:24,290 --> 00:04:28,070
+البكتيريا، شو العوامل التي بتأثر على النمو بشكل
+
+65
+00:04:28,070 --> 00:04:36,570
+عام، كيف بدي أقيس مدى النمو. هذا طبعا مواضيع المساق
+
+66
+00:04:36,570 --> 00:04:42,170
+وتوزيعها خلال الفصل. بالنسبة لتوزيع الدرجات، هيكون
+
+67
+00:04:42,170 --> 00:04:47,370
+طبعا ثلاثين عملي، وسبعين نهائي. في عندي quizات
+
+68
+00:04:47,370 --> 00:04:51,550
+عشرين درجة، هيكون quizان. كل quiz هيكون في ثلاث
+
+69
+00:04:51,550 --> 00:04:56,390
+معامل. كل quiz هيكون من عشرين، وهنقسم الدرجة على
+
+70
+00:04:56,390 --> 00:05:02,090
+اثنين،  عشرة بيطلع عشرة. لكل quiz homework عليه
+
+71
+00:05:02,090 --> 00:05:06,010
+عشر علامات. هيكون homework واحد فقط، هنحكي عنه في
+
+72
+00:05:06,010 --> 00:05:09,130
+حينه، هيكون شوية يعني بعد ما نخلص الفحوصات
+
+73
+00:05:09,130 --> 00:05:15,250
+الكيميائية. الـ final exam هيكون سبعين، ثلاثين عملي و
+
+74
+00:05:15,250 --> 00:05:20,250
+أربعين نظري. بالنهاية طبعا مجموع العلامات مئة. هيك
+
+75
+00:05:20,250 --> 00:05:26,150
+بيكون توزيع الدرجات. بتمنى تكون الأمور واضحة و
+
+76
+00:05:26,150 --> 00:05:29,590
+بتمنى لكم كل التوفيق. والسلام عليكم ورحمة الله
+
+77
+00:05:29,590 --> 00:05:30,450
+وبركاته
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/hwINTbSvNaQ_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/hwINTbSvNaQ_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..83755e0f9de3a4b63e44dcba6dc5aa6b00662e3b
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/hwINTbSvNaQ_postprocess.srt
@@ -0,0 +1,308 @@
+1
+00:00:00,670 --> 00:00:04,490
+في الأسبوع السابع والتامن والتاسع والعاشر هذه
+
+2
+00:00:04,490 --> 00:00:08,670
+الأسبوع كلها هناخد ال biochemical test الفحوصات
+
+3
+00:00:08,670 --> 00:00:13,250
+الكيميائية أنا عشان أوصل لتشخيص الصح لازم أجري هذه
+
+4
+00:00:13,250 --> 00:00:17,320
+الفحوصاتقبل ما اجري اي فحص لازم يكون فيه اندي انا
+
+5
+00:00:17,320 --> 00:00:21,300
+pure culture طبعا ال pure culture طبعا احنا قلنا
+
+6
+00:00:21,300 --> 00:00:26,220
+انه كل المستعمرة على الطبق ماخدة نفس الشكل اي انه
+
+7
+00:00:26,220 --> 00:00:31,280
+اصل الكولوني هو خلية بكتيرية واحدة هنتعرف على كل
+
+8
+00:00:31,280 --> 00:00:36,500
+فحص بالتفصيل هنتعرف على ال principle الفحص هنتعرف
+
+9
+00:00:36,500 --> 00:00:40,920
+علىالـ Significant أهمية كل فحص، كيف بنعمل الفحص،
+
+10
+00:00:40,920 --> 00:00:43,840
+شو النتيجة الـ Positive، كيف بتكون، شو النتيجة الـ
+
+11
+00:00:43,840 --> 00:00:47,500
+Negative، كيف بتكون شكلها، مين الـ Positive لهذا
+
+12
+00:00:47,500 --> 00:00:51,120
+الفحص من البكتيريا ومين الـ Negative لكل فحص من
+
+13
+00:00:51,120 --> 00:00:55,180
+البكتيريا بالنسبة للأسبوع الـ 11، في الأسبوع الـ
+
+14
+00:00:55,180 --> 00:00:58,980
+11 هنتعرف على الـ Bacterial Oxygen Requirement and
+
+15
+00:00:58,980 --> 00:01:02,640
+Anaerobic Bacteriaطبعا البكتيريا منصنفها حسب
+
+16
+00:01:02,640 --> 00:01:06,620
+حجبتها لأكسوجين بكتيريا هوائية بكتيريا لا هوائية
+
+17
+00:01:06,620 --> 00:01:10,820
+بكتيريا بتحتها الـCO2 مثلا فبالتالي انا لما بدي
+
+18
+00:01:10,820 --> 00:01:14,160
+اعزل بكتيريا X وانا عارفة ان بكتيريا X بتحتها
+
+19
+00:01:14,160 --> 00:01:17,900
+الـCO2 فلازم اوفر ظروف الـCO2 حتى تنمو لو انا
+
+20
+00:01:17,900 --> 00:01:21,240
+ماوفرت هذه الظروف البكتيريا مش هتنمو فهذا المعمل
+
+21
+00:01:21,240 --> 00:01:26,040
+هنتعرف كيف نوفر مثلا ظروف هوائية كيف نوفر ظروف لا
+
+22
+00:01:26,040 --> 00:01:30,070
+هوائية كيف انا بدي أوفر ظروف CO2في الأسبوع الـ 12
+
+23
+00:01:30,070 --> 00:01:34,810
+هنتعرف single media multiple test طبعا هذا يتبع لل
+
+24
+00:01:34,810 --> 00:01:38,890
+biochemical test انا في ال biochemical test لو بدي
+
+25
+00:01:38,890 --> 00:01:43,350
+اعمل مثلا عشان اوصل لتشخيص الصحة اكيد هعمل اكتر من
+
+26
+00:01:43,350 --> 00:01:47,010
+فحص كيميائي لان كل ما زادت عدد الفحوصات كل ما زادت
+
+27
+00:01:47,010 --> 00:01:52,610
+الدقة في التشخيص تخيلوا انا بديأشخص بكتيريا معينة
+
+28
+00:01:52,610 --> 00:01:57,850
+و بدي اعمل فحص كذا فحص بالتالي هياخد مني وقت و
+
+29
+00:01:57,850 --> 00:02:02,290
+هياخد مني مجهود ان انا كل فحص بده أدوات خاصة بدي
+
+30
+00:02:02,290 --> 00:02:07,250
+احضر لكل فحص مثلا media خاصة هذا هياخد مني وقت و
+
+31
+00:02:07,250 --> 00:02:10,870
+جهدفكرة الـ Single Media Multiple Test انه ميديا
+
+32
+00:02:10,870 --> 00:02:15,670
+واحدة بزرع البكتيريا اللي انا بدي طبعا اعرف او بدي
+
+33
+00:02:15,670 --> 00:02:20,030
+اشخصها و اعرف هويتها و تاني يوم باجي بشوف النتائج
+
+34
+00:02:20,030 --> 00:02:25,850
+بكون طبعا النتيجة بعد 24 ساعة بقرأ نتيجة طبعا اكتر
+
+35
+00:02:25,850 --> 00:02:30,090
+من فحص في نفس الميديا في الأسبوع التلتاشر في اشي
+
+36
+00:02:30,090 --> 00:02:33,030
+اسمه هناخد معملين طبعا Selective and Differential
+
+37
+00:02:33,030 --> 00:02:36,390
+Media وفي معمل تاني منفصل عنه اللي هو Serial
+
+38
+00:02:36,390 --> 00:02:39,870
+Dilutionبالنسبة لـ Selective and Differential
+
+39
+00:02:39,870 --> 00:02:42,990
+Media قولنا ميديا هو وسط غذائي الوسط الغذائي في
+
+40
+00:02:42,990 --> 00:02:45,570
+عندنا إيه اللي هو تصنيفات مختلفة هنتعرف في هذا
+
+41
+00:02:45,570 --> 00:02:49,070
+المعمل عن هذه التصنيفات في من ضمن التصنيفات إش
+
+42
+00:02:49,070 --> 00:02:52,450
+اسمه اللي هو الـ Selective Media الـ Selective
+
+43
+00:02:52,450 --> 00:02:58,840
+معناها اللي هي انتقائي أو اختيارييعني أنا بدي أنمي
+
+44
+00:02:58,840 --> 00:03:03,480
+مثلا الـ bacteria اللي هي الـ Mojapetgram في عندي
+
+45
+00:03:03,480 --> 00:03:07,960
+ميديا خاصة بتنمي فقط الـ Mojapetgram و بتطبط الـ
+
+46
+00:03:07,960 --> 00:03:11,320
+Celebitgram طبعا أكيد في الميديا في مكون بيعمل على
+
+47
+00:03:11,320 --> 00:03:16,540
+تثبيت مثلا الـ Celebitgramهنتعرف على أنواع متعددة
+
+48
+00:03:16,540 --> 00:03:19,760
+من الـ Media Selective الـ Differential Media إن
+
+49
+00:03:19,760 --> 00:03:24,440
+هي بتنمي أكتر منه لكن بتعطي مثلا بكتيريا X لون على
+
+50
+00:03:24,440 --> 00:03:29,440
+هذا الوسط وبتعطي بكتيريا Y لون تاني على نفس الوسط
+
+51
+00:03:29,440 --> 00:03:33,140
+يمكن شفته في صورة الـ group الـ WhatsApp فيه
+
+52
+00:03:33,140 --> 00:03:38,500
+انديانطبق مقسم نصين نصه مزروع عليه بكتيريا X هو
+
+53
+00:03:38,500 --> 00:03:43,770
+النص التاني مزروع عليه بكتيريا YX أعطت لون أصفر وY
+
+54
+00:03:43,770 --> 00:03:46,590
+أعطت لون colorless فهذه تعتبر الـ differential
+
+55
+00:03:46,590 --> 00:03:50,570
+media الـ cereal dilution طبعا احنا قلنا ان
+
+56
+00:03:50,570 --> 00:03:56,750
+البكتيريا تعتبر ما بشوفها الا على شكل مستعمرات
+
+57
+00:03:56,750 --> 00:04:01,350
+فبالتالي انا لو بدي اعد البكتيريا مش هقدر اعد
+
+58
+00:04:01,350 --> 00:04:05,370
+البكتيريا لأن الكلوني هو تجمع ملايين من الخلايا
+
+59
+00:04:05,370 --> 00:04:08,910
+البكتيرية في هذا المعمل هنتعرف كيف انا لو بدي اعد
+
+60
+00:04:08,910 --> 00:04:12,510
+البكتيريا مثلا بدي اشوفمدى استجابة المريض للـ
+
+61
+00:04:12,510 --> 00:04:16,170
+Antibiotic هلقل ولا زاد هنعرف من خلال الـ Serial
+
+62
+00:04:16,170 --> 00:04:20,590
+Dilution في الأسبوع الـ 14 هنعرف لـ Growth Curve
+
+63
+00:04:20,590 --> 00:04:24,290
+and Microbial Control Agents هنتعرف على نمو
+
+64
+00:04:24,290 --> 00:04:28,070
+البكتيريا شو العوامل اللتي بتأثر على النمو بشكل
+
+65
+00:04:28,070 --> 00:04:36,570
+عام كيف بدي أقيس مدى النمو هذا طبعا مواضيع المساق
+
+66
+00:04:36,570 --> 00:04:42,170
+ويتوزيها خلال الفصلبالنسبة لتوزيع الدرجات هيكون
+
+67
+00:04:42,170 --> 00:04:47,370
+طبعا تلاتين و نص في و سبعين نهائي في عندي quizات
+
+68
+00:04:47,370 --> 00:04:51,550
+عشرين درجة هيكون quizان كل quiz هيكون في تلات
+
+69
+00:04:51,550 --> 00:04:56,390
+معامل كل quiz هيكون من عشرين و هنقسم الدرجة على
+
+70
+00:04:56,390 --> 00:05:02,090
+اتنين عشرة بيطلع عشرة في لكل quiz homework عليا
+
+71
+00:05:02,090 --> 00:05:06,010
+عشر علامات هيكون homework واحد فقط هنحكي عنه في
+
+72
+00:05:06,010 --> 00:05:09,130
+حينه هيكون شويه يعنيبعد ما نخلص الفحوزات
+
+73
+00:05:09,130 --> 00:05:15,250
+الكيميائية ال final exam هيكون سبعين تلاتين عملي و
+
+74
+00:05:15,250 --> 00:05:20,250
+أربعين نظري بالنهاية طبعا مجموع العلمات مية هيك
+
+75
+00:05:20,250 --> 00:05:26,150
+بيكون توزيع الدرجات بتمنى تكون الأمور واضحة و
+
+76
+00:05:26,150 --> 00:05:29,590
+بتمنى لكوا كل التوفيق والسلام عليكم ورحمة الله
+
+77
+00:05:29,590 --> 00:05:30,450
+وبركاته
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/hwINTbSvNaQ_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/hwINTbSvNaQ_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..e7328ce852a77f290937eee838314c2a18a064eb
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/hwINTbSvNaQ_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 1391, "start": 0.67, "end": 13.91, "text": "في الأسبوع السابع والتامن والتاسع والعاشر هذه الأسبوع كلها هناخد ال biochemical test الفحوصات الكيميائية أنا عشان أوصل لتشخيص الصح لازم أجري هذه الفحوصات", "tokens": [41185, 16247, 35457, 45367, 21136, 16758, 3615, 16070, 2655, 10943, 1863, 16070, 2655, 32277, 3615, 16070, 3615, 33599, 2288, 29538, 16247, 35457, 45367, 28242, 11296, 8032, 1863, 47283, 3215, 2423, 12198, 34114, 1500, 27188, 5016, 2407, 9381, 9307, 33251, 32640, 1829, 16373, 10632, 41850, 6225, 8592, 7649, 34051, 36520, 5296, 2655, 8592, 9778, 1829, 9381, 31767, 5016, 5296, 31377, 2304, 5551, 7435, 16572, 29538, 27188, 5016, 2407, 9381, 9307], "avg_logprob": -0.115401783904859, "compression_ratio": 1.6441717791411044, "no_speech_prob": 3.3974647521972656e-06, "words": [{"start": 0.67, "end": 0.81, "word": "في", "probability": 0.7236328125}, {"start": 0.81, "end": 1.31, "word": " الأسبوع", "probability": 0.9884440104166666}, {"start": 1.31, 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"probability": 0.91689453125}, {"start": 9.81, "end": 10.39, "word": " أنا", "probability": 0.59619140625}, {"start": 10.39, "end": 10.69, "word": " عشان", "probability": 0.9835611979166666}, {"start": 10.69, "end": 11.13, "word": " أوصل", "probability": 0.845703125}, {"start": 11.13, "end": 11.73, "word": " لتشخيص", "probability": 0.8926595052083334}, {"start": 11.73, "end": 12.11, "word": " الصح", "probability": 0.9853515625}, {"start": 12.11, "end": 12.59, "word": " لازم", "probability": 0.9840494791666666}, {"start": 12.59, "end": 12.99, "word": " أجري", "probability": 0.7936197916666666}, {"start": 12.99, "end": 13.25, "word": " هذه", "probability": 0.70947265625}, {"start": 13.25, "end": 13.91, "word": " الفحوصات", "probability": 0.99169921875}], "temperature": 1.0}, {"id": 2, "seek": 3678, "start": 14.34, "end": 36.78, "text": "قبل ما اجري اي فحص لازم يكون فيه اندي انا pure culture طبعا ال pure culture طبعا احنا قلنا انه كل المستعمرة على الطبق ماخدة نفس الشكل اي انه اصل الكولوني 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2288, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224], "avg_logprob": -0.1704673495915559, "compression_ratio": 1.7433628318584071, "no_speech_prob": 0.0008463859558105469, "words": [{"start": 307.71, "end": 308.21, "word": "بعد", "probability": 0.689697265625}, {"start": 308.21, "end": 308.31, "word": " ما", "probability": 0.2890625}, {"start": 308.31, "end": 308.65, "word": " نخلص", "probability": 0.8944091796875}, {"start": 308.65, "end": 309.13, "word": " الفحوزات", "probability": 0.91572265625}, {"start": 309.13, "end": 309.93, "word": " الكيميائية", "probability": 0.9173828125}, {"start": 309.93, "end": 310.97, "word": " ال", "probability": 0.8212890625}, {"start": 310.97, "end": 311.61, "word": " final", "probability": 0.615234375}, {"start": 311.61, "end": 311.97, "word": " exam", "probability": 0.974609375}, {"start": 311.97, "end": 312.53, "word": " هيكون", "probability": 0.833740234375}, {"start": 312.53, "end": 313.17, "word": " سبعين", "probability": 0.80206298828125}, {"start": 313.17, "end": 314.49, "word": " تلاتين", "probability": 0.86572265625}, {"start": 314.49, "end": 314.91, "word": " عملي", "probability": 0.9607747395833334}, {"start": 314.91, "end": 315.25, "word": " و", "probability": 0.896484375}, {"start": 315.25, "end": 315.87, "word": " أربعين", "probability": 0.8836669921875}, {"start": 315.87, "end": 316.29, "word": " نظري", "probability": 0.978515625}, {"start": 316.29, "end": 317.53, "word": " بالنهاية", "probability": 0.9017333984375}, {"start": 317.53, "end": 317.89, "word": " طبعا", "probability": 0.84088134765625}, {"start": 317.89, "end": 318.25, "word": " مجموع", "probability": 0.916748046875}, {"start": 318.25, "end": 318.93, "word": " العلمات", "probability": 0.8282877604166666}, {"start": 318.93, "end": 319.41, "word": " مية", "probability": 0.672607421875}, {"start": 319.41, "end": 320.25, "word": " هيك", "probability": 0.73779296875}, {"start": 320.25, "end": 320.73, "word": " بيكون", "probability": 0.74658203125}, {"start": 320.73, "end": 321.29, "word": " توزيع", "probability": 0.9134114583333334}, {"start": 321.29, "end": 322.21, "word": " الدرجات", "probability": 0.93896484375}, {"start": 322.21, "end": 324.15, "word": " بتمنى", "probability": 0.69598388671875}, {"start": 324.15, "end": 324.57, "word": " تكون", "probability": 0.70556640625}, {"start": 324.57, "end": 325.03, "word": " الأمور", "probability": 0.97705078125}, {"start": 325.03, "end": 325.85, "word": " واضحة", "probability": 0.971923828125}, {"start": 325.85, "end": 326.15, "word": " و", "probability": 0.81884765625}, {"start": 326.15, "end": 326.91, "word": " بتمنى", "probability": 0.849609375}, {"start": 326.91, "end": 327.15, "word": " لكوا", "probability": 0.7340494791666666}, {"start": 327.15, "end": 327.35, "word": " كل", "probability": 0.9755859375}, {"start": 327.35, "end": 327.99, "word": " التوفيق", "probability": 0.96484375}, {"start": 327.99, "end": 328.39, "word": " والسلام", "probability": 0.9147135416666666}, {"start": 328.39, "end": 328.87, "word": " عليكم", "probability": 0.93212890625}, {"start": 328.87, "end": 329.25, "word": " ورحمة", "probability": 0.9478759765625}, {"start": 329.25, "end": 329.59, "word": " الله", "probability": 0.97021484375}, {"start": 329.59, "end": 330.45, "word": " وبركاته", "probability": 0.98125}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 330.97725, "duration_after_vad": 326.83375}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/kghd40S327U.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/kghd40S327U.srt
new file mode 100644
index 0000000000000000000000000000000000000000..eef0e5591dbbf2b9b475776316fe9339e0edd75d
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/kghd40S327U.srt
@@ -0,0 +1,1401 @@
+1
+00:00:02,530 --> 00:00:06,450
+السلام عليكم ورحمة الله وبركاته، يعطيكم العافية
+
+2
+00:00:06,450 --> 00:00:10,410
+جميعًا في هذا اللقاء، ستكون المحاضرة بعنوان الـ
+
+3
+00:00:10,410 --> 00:00:13,790
+Serial Dilution Method of Bacteria Enumeration
+
+4
+00:00:13,790 --> 00:00:18,870
+طريقة عد البكتيريا باستخدام الـ Serial Dilution
+
+5
+00:00:18,870 --> 00:00:23,710
+Method. طبعًا سنذكر أكثر من طريقة لعد البكتيريا، لكن
+
+6
+00:00:23,710 --> 00:00:27,550
+في هذا المعمل سنشرح طريقة الـ Serial Dilution
+
+7
+00:00:27,550 --> 00:00:31,490
+Method. Many studies require the quantitative
+
+8
+00:00:32,150 --> 00:00:35,470
+determination of bacterial population، واللي في
+
+9
+00:00:35,470 --> 00:00:39,950
+كثير من الدراسات تحتاج أن أعد البكتيريا
+
+10
+00:00:39,950 --> 00:00:43,410
+quantitative determination of bacterial population
+
+11
+00:00:43,410 --> 00:00:48,410
+quantitative، يعني عد البكتيريا، لكن لما أنا أحكي
+
+12
+00:00:48,410 --> 00:00:53,170
+qualitative، qualitative يعني presence or absence
+
+13
+00:00:53,170 --> 00:00:56,790
+أريد أن أشوف هل البكتيريا موجودة أم لا، لكن
+
+14
+00:00:56,790 --> 00:01:01,340
+quantitative، أتحدث عن عد البكتيريا، أقول في عندي
+
+15
+00:01:01,340 --> 00:01:05,540
+طريقتان لعد البكتيريا: the two most widely used
+
+16
+00:01:05,540 --> 00:01:10,240
+methods for determining bacterial numbers are
+
+17
+00:01:10,240 --> 00:01:14,540
+الطريقتان اللتان أستخدمهما في عد البكتيريا: الـ
+
+18
+00:01:14,540 --> 00:01:18,560
+most common. أول طريقة، وهي الـ Standard Plate
+
+19
+00:01:18,560 --> 00:01:22,820
+Count method، التي سنتحدث عنها في هذا المعمل، وهي
+
+20
+00:01:22,820 --> 00:01:27,300
+Serial Dilution method. الطريقة الثانية: الـ
+
+21
+00:01:27,300 --> 00:01:31,880
+Spectrophotometer،  الـ Turbidimetric Analysis
+
+22
+00:01:31,880 --> 00:01:37,040
+تعتمد على الـ Turbidity. طبعًا هذه الطريقة سنذكرها
+
+23
+00:01:37,040 --> 00:01:41,800
+في الفصل المخصص لها في chapter، وهو الـ
+
+24
+00:01:41,800 --> 00:01:44,040
+bacterial growth، وهي طريقة الـ
+
+25
+00:01:44,040 --> 00:01:49,160
+Spectrophotometer. أقول لكم طريقة الـ Standard Plate
+
+26
+00:01:49,160 --> 00:01:53,440
+Count method أو Serial Dilution method is an
+
+27
+00:01:53,440 --> 00:01:57,840
+indirect measurement of cell density. هي طريقة
+
+28
+00:01:57,840 --> 00:01:58,620
+indirect
+
+29
+00:02:10,430 --> 00:02:14,650
+indirect measurement. لماذا قال لي طريقة غير مباشرة؟
+
+30
+00:02:14,650 --> 00:02:21,310
+لأن التعبير عن عدد البكتيريا يكون في نهاية
+
+31
+00:02:21,310 --> 00:02:28,070
+الحل: Colony Forming Unit. الوحدة التي أعبر عنها
+
+32
+00:02:28,070 --> 00:02:33,150
+هي Colony Forming Unit per ml أو جرام حسب العينة.
+
+33
+00:02:33,530 --> 00:02:37,430
+طيب، كولوني يعني مستعمرة. المستعمرة لا أعرف كم
+
+34
+00:02:37,430 --> 00:02:40,930
+البكتيريا الموجودة فيها. قد تكون single، قد تكون
+
+35
+00:02:40,930 --> 00:02:45,570
+chain. بالتالي، أعبر بـ Colony Forming Unit، لأن
+
+36
+00:02:45,570 --> 00:02:48,890
+بهذا أسمي هذه الطريقة، أو أحكي عن هذه الطريقة
+
+37
+00:02:48,890 --> 00:02:54,310
+كطريقة Indirect Measurement. طيب، لماذا قال لي إنها فقط تقيس
+
+38
+00:02:54,310 --> 00:02:58,800
+البكتيريا الحية؟ نعدّ في الـ
+
+39
+00:02:58,800 --> 00:03:02,080
+Standard Plate Count method فقط البكتيريا الحية،
+
+40
+00:03:02,080 --> 00:03:06,280
+لأن الميتة لن تتكاثر، لأنها تعتمد على الـ
+
+41
+00:03:06,280 --> 00:03:10,620
+Plate Count method.  الـ Plate Count method أنني
+
+42
+00:03:10,620 --> 00:03:16,960
+أزرع، أو أعمل تخفيفًا للعينة، ثم أزرع كل تخفيف
+
+43
+00:03:16,960 --> 00:03:21,800
+على طبق وأضعها في الحاضنة، بالتالي البكتيريا
+
+44
+00:03:21,800 --> 00:03:26,850
+الميتة لن تتكاثر في هذا الطبق. لهذا السبب فقط في هذه
+
+45
+00:03:26,850 --> 00:03:30,990
+الطريقة نقيس، أو نعد البكتيريا الحية.
+
+46
+00:03:30,990 --> 00:03:36,690
+طيب، الطريقة الثانية: Spectrophotometer analysis،
+
+47
+00:03:36,690 --> 00:03:40,130
+تقول إنها تعتمد على الـ Turbidity، لهذا السبب اسمها
+
+48
+00:03:40,130 --> 00:03:46,110
+Turbidimetric analysis أو Spectrophotometer. أقول
+
+49
+00:03:46,110 --> 00:03:50,760
+إنها تعتمد على الـ Turbidity، indirectly measured أيضًا.
+
+50
+00:03:50,760 --> 00:03:54,060
+كـ Standard Plate Count، هي تعتبر Indirect
+
+51
+00:03:54,060 --> 00:03:58,420
+measurement. لماذا؟ لأنها تعتمد على الـ Turbidity.
+
+52
+00:03:58,420 --> 00:04:03,200
+طبعًا، العكارة لا أستطيع أن أميز البكتيريا الحية
+
+53
+00:04:03,200 --> 00:04:06,520
+والبكتيريا الميتة. لما يكون في نمو على شكل
+
+54
+00:04:06,520 --> 00:04:10,180
+growth، على شكل عكارة، على شكل Turbidity، لا
+
+55
+00:04:10,180 --> 00:04:14,810
+أستطيع أن أميز هل البكتيريا حية أم ميتة. البكتيريا سواء
+
+56
+00:04:14,810 --> 00:04:18,390
+كانت حية أم ميتة، يكون عندي Turbidity، يكون عندي
+
+57
+00:04:18,390 --> 00:04:23,630
+عكارة، تمام؟ يبقى سواء كانت طبعًا هي تحت
+
+58
+00:04:23,630 --> 00:04:27,190
+المجهر، البكتيريا، أو عندي عكارة، لا أستطيع أن أميز
+
+59
+00:04:27,190 --> 00:04:30,450
+بين البكتيريا الحية والميتة، لكن على الطبق فقط
+
+60
+00:04:30,450 --> 00:04:35,070
+البكتيريا الحية هي التي تتكاثر. يبقى بطريقة الـ
+
+61
+00:04:35,070 --> 00:04:38,940
+Standard Plate Count أعد البكتيريا الحية. في
+
+62
+00:04:38,940 --> 00:04:43,900
+طريقة الـ Spectrophotometer أقيس البكتيريا الحية
+
+63
+00:04:43,900 --> 00:04:50,000
+والميتة، تمام؟ هذا بالنسبة للطريقتين. الطريقتان طبعًا
+
+64
+00:04:50,000 --> 00:04:54,540
+هما لعد البكتيريا، الـ Quantitative.  طب، نجي لأول
+
+65
+00:04:54,540 --> 00:04:59,380
+طريقة التي سنشرحها في هذا المعمل،
+
+66
+00:04:59,380 --> 00:05:03,440
+وهي الـ Standard Plate Count method، التي أسميها
+
+67
+00:05:03,440 --> 00:05:04,820
+viable count.
+
+68
+00:05:09,180 --> 00:05:13,840
+The Plate Count, viable count، يعني حية، لأنها تعد
+
+69
+00:05:13,840 --> 00:05:16,760
+البكتيريا الحية.  يبقى لها اسم آخر، وهو الـ
+
+70
+00:05:16,760 --> 00:05:21,680
+viable count، لأنها تعد فقط البكتيريا الحية. The
+
+71
+00:05:21,680 --> 00:05:25,500
+number of bacteria in a given sample is usually
+
+72
+00:05:25,500 --> 00:05:30,440
+too great to be counted directly. أقول لكم البكتيريا
+
+73
+00:05:30,440 --> 00:05:35,300
+أو عدد البكتيريا في عينة معينة يكون كبير جدًا
+
+74
+00:05:35,300 --> 00:05:39,970
+بحيث لا أقدر أن أعدها. بالتالي، لو ذهبتِ وأخذتِ من
+
+75
+00:05:39,970 --> 00:05:44,650
+العينة الأصلية، وزرعتِها على الطبق، سيكون كل
+
+76
+00:05:44,650 --> 00:05:48,890
+الطبق مليئًا بمستعمرات بكتيرية. بالتالي، أنا لا أقدر
+
+77
+00:05:48,890 --> 00:05:54,510
+أن أعد، لا أعرف كيف أعد هذه الـ Colonies. لهذا السبب نلجأ
+
+78
+00:05:54,510 --> 00:05:58,850
+إلى طريقة التخفيف. However, if the sample is
+
+79
+00:05:58,850 --> 00:06:03,110
+serially diluted. أقول لكم أن العينة نقوم بعمل
+
+80
+00:06:03,110 --> 00:06:08,040
+تخفيف لها، حتى أقدر أن أعدها، تمام؟  يجب أن نخفف
+
+81
+00:06:08,040 --> 00:06:12,080
+العينة الأصلية، بالتالي يكون العدد قليلًا، أقدر أن
+
+82
+00:06:12,080 --> 00:06:17,080
+أعدها، ثم أضرب في العامل، وهو
+
+83
+00:06:17,080 --> 00:06:23,280
+ما هو التخفيف؟ ما هو الـ Dilution؟ أضرب فيه العامل. And
+
+84
+00:06:23,280 --> 00:06:27,740
+then blotted out on an agar surface in such a manner
+
+85
+00:06:27,740 --> 00:06:31,020
+that single isolated bacteria form visible
+
+86
+00:06:31,020 --> 00:06:35,460
+isolated colonies. أقول لكم بعد أن تعملي Serial Dilution،
+
+87
+00:06:35,460 --> 00:06:40,700
+تعملي تخفيفًا للعينة الأصلية، آخذ من كل
+
+88
+00:06:40,700 --> 00:06:47,200
+تخفيف حجمًا معينًا، تقريبًا كنا نأخذ ما هو 1 من
+
+89
+00:06:47,200 --> 00:06:52,720
+عشرة مل من كل تخفيف، أروح أزرعه على طبق، تمام؟
+
+90
+00:06:52,720 --> 00:06:57,980
+ثم أفرد هذا الحجم، 1 من 10 مل، أفرده على
+
+91
+00:06:57,980 --> 00:07:03,380
+الطبق باستخدام المِشْحَة.  ممكن كنا نعمل من كل أنبوب
+
+92
+00:07:03,380 --> 00:07:08,000
+two plates، طبقين، وبعدها آخذ الـ
+
+93
+00:07:08,000 --> 00:07:11,320
+average بينهما، ممكن أعمل على طبق واحد، أزرع كل أنبوب
+
+94
+00:07:11,320 --> 00:07:14,780
+كل تخفيف على طبق واحد، أو ممكن على طبقين، و
+
+95
+00:07:14,780 --> 00:07:19,140
+بعدها آخذ الـ average. نضعها في المحضنة، ونضعها في
+
+96
+00:07:19,140 --> 00:07:23,240
+الحاضنة، وبعد 24 ساعة، أشوف العدّ، أشوف
+
+97
+00:07:23,240 --> 00:07:29,030
+العدد. أقول لكم كل single isolated bacteria تكون من
+
+98
+00:07:29,030 --> 00:07:32,870
+isolated colony، تعبر عن isolated colony. The
+
+99
+00:07:32,870 --> 00:07:36,430
+number of colonies can be used as a measure of the
+
+100
+00:07:36,430 --> 00:07:40,050
+number of viable living cells in the dilution.
+
+101
+00:07:40,050 --> 00:07:44,730
+أقول لكم عدد الـ colonies التي أراها بعد أن
+
+102
+00:07:44,730 --> 00:07:51,190
+أضعها في الحاضنة سيعبر عن عدد الخلايا الحية في
+
+103
+00:07:51,190 --> 00:07:55,010
+هذا التخفيف، في هذا التخفيف الذي أخذت منه
+
+104
+00:07:55,010 --> 00:08:02,830
+وزرعته على الطبق، تمام؟ هذا بالنسبة لأول شريحة، نجي
+
+105
+00:08:02,830 --> 00:08:06,610
+Keep in mind that if the organism normally forms
+
+106
+00:08:06,610 --> 00:08:10,210
+multiple cell arrangements such as chains, the
+
+107
+00:08:10,210 --> 00:08:13,830
+colony forming unit may consist of a chain of
+
+108
+00:08:13,830 --> 00:08:18,030
+bacteria rather than a single bacterium. أقول لكم أنّه
+
+109
+00:08:18,350 --> 00:08:21,810
+قلنا في أول معمل، عندما تعرفنا على الشكل، قلنا
+
+110
+00:08:21,810 --> 00:08:25,870
+البكتيريا قد تكون، عندي أيضًا Arrangement، ترتيب
+
+111
+00:08:25,870 --> 00:08:30,950
+للخلايا البكتيرية، حسب مستوى الانقسام، قد
+
+112
+00:08:30,950 --> 00:08:34,870
+تكون البكتيريا على شكل سلاسل، على شكل سلسلة، قد تكون
+
+113
+00:08:34,870 --> 00:08:39,830
+على شكل تجمعات، تمام؟ بالتالي، لما أعبر بالـ
+
+114
+00:08:39,830 --> 00:08:43,710
+Colony Forming Unit، الـ Colony Forming Unit قلنا
+
+115
+00:08:43,710 --> 00:08:47,790
+قد تكون الكولوني تجمعًا من الخلايا البكتيرية، الـ
+
+116
+00:08:47,790 --> 00:08:51,190
+single cell. أقول هنا ليس بالضرورة أن تكون
+
+117
+00:08:51,190 --> 00:08:55,570
+على شكل single bacterium، قد تكون chain of
+
+118
+00:08:55,570 --> 00:09:00,910
+بكتيريا، بالتالي هنا أعتبر القياس
+
+119
+00:09:00,910 --> 00:09:05,300
+indirect. أقول لكم In addition, some of the bacteria may
+
+120
+00:09:05,300 --> 00:09:09,120
+be clumped together.  أيضًا، قد تتجمع البكتيريا مع
+
+121
+00:09:09,120 --> 00:09:12,820
+بعضها، كما قلنا قد تكون على شكل أسطاف، على شكل
+
+122
+00:09:12,820 --> 00:09:17,480
+عنقود عنب. بالتالي، Therefore, we are doing the
+
+123
+00:09:17,480 --> 00:09:21,720
+Plate Count technique، نقول عمومًا إننا نحدد
+
+124
+00:09:21,720 --> 00:09:24,800
+عدد Colony Forming Units في
+
+125
+00:09:24,800 --> 00:09:28,600
+هذا التخفيف. لهذا السبب، لما أعمل طريقة الـ
+
+126
+00:09:28,600 --> 00:09:33,810
+Serial Dilution أو Plate Count technique، أعبر
+
+127
+00:09:33,810 --> 00:09:38,390
+في النهاية عن العدد بـ Colony Forming Units، لا أحكي
+
+128
+00:09:38,390 --> 00:09:41,610
+single bacterium أو each bacterium، لأن في النهاية
+
+129
+00:09:41,610 --> 00:09:46,270
+المستعمرة قد تكون عبارة عن single bacterium، قد تكون
+
+130
+00:09:46,270 --> 00:09:50,390
+each، قد تكون clump. فأعبر عن Colony
+
+131
+00:09:50,390 --> 00:09:54,770
+Forming Units، وهو المستعمرة، عدد المستعمرات التي
+
+132
+00:09:54,770 --> 00:10:00,480
+عدّيتها في هذه الأطباق، التي زرعتها من
+
+133
+00:10:00,480 --> 00:10:04,780
+هذا التخفيف، in that dilution، من هذا التخفيف.
+
+134
+00:10:04,780 --> 00:10:10,300
+By extrapolation, this number can in turn be used
+
+135
+00:10:10,300 --> 00:10:13,420
+to calculate the number of colony forming units in
+
+136
+00:10:13,420 --> 00:10:18,120
+the original sample. طبعًا، أعدّ عدد، أعدّ الكولونيات،
+
+137
+00:10:18,120 --> 00:10:23,000
+وفي النهاية أعبر عن، التي هي الوحدة بكولوني
+
+138
+00:10:23,000 --> 00:10:27,690
+Forming Units في هذا، in the original sample، بعد أن
+
+139
+00:10:27,690 --> 00:10:31,930
+أعيد التخفيف، أضرب في ما هو تخفيف العينة حتى أحصل
+
+140
+00:10:31,930 --> 00:10:37,190
+على عدد البكتيريا في العينة الأصلية. Bacterial
+
+141
+00:10:37,190 --> 00:10:42,570
+count by this method are usually expressed as
+
+142
+00:10:42,570 --> 00:10:46,490
+colony forming units per milliliter. أقول لكم عدد
+
+143
+00:10:46,490 --> 00:10:49,930
+البكتيريا في هذه الطريقة أعبر عنها بـ Colony
+
+144
+00:10:49,930 --> 00:10:55,170
+Forming Units per ml أو جرام حسب نوع العينة، إذا
+
+145
+00:10:55,170 --> 00:11:00,850
+كانت سائلة أعبر بالملي لتر، وإذا كانت صلبة أعبر
+
+146
+00:11:00,850 --> 00:11:04,950
+بجرام. يبقى كلها uniform unit per ml أو جرام
+
+147
+00:11:04,950 --> 00:11:06,370
+حسب نوع العينة.
+
+148
+00:11:10,090 --> 00:11:14,070
+Normally, the bacterial sample is diluted by a factor
+
+149
+00:11:14,070 --> 00:11:19,530
+of 10 and plated on agar. أقول لكم عادةً لما أعمل
+
+150
+00:11:19,530 --> 00:11:25,490
+تخفيفًا للعينة الأصلية، أقوم بعمل مضاعفات العشرة،
+
+151
+00:11:25,490 --> 00:11:30,690
+وأزرعها على الـ agar. After incubation, the number
+
+152
+00:11:30,690 --> 00:11:36,090
+of colonies on the dilution plates showing between. بعد
+
+153
+00:11:36,090 --> 00:11:40,560
+ما أعمل incubation للأطباق التي أخذت من
+
+154
+00:11:40,560 --> 00:11:46,500
+كل تخفيف حجمًا معينًا، وذهبت وزرعتُه على طبق، أو
+
+155
+00:11:46,500 --> 00:11:50,920
+طبقين من كل تخفيف. بعد الـ incubation، من عدّ هذه الـ
+
+156
+00:11:50,920 --> 00:11:55,800
+colonies، أي الأطباق التي أريد أن أعدّها؟ لو كان فقط
+
+157
+00:11:55,800 --> 00:12:01,380
+كان عدد الـ colonies ما بين 30 إلى 300 colony، هو هذا
+
+158
+00:12:01,380 --> 00:12:06,700
+الذي أريد أن أعوضه في النهاية في القانون. هي تقول
+
+159
+00:12:06,700 --> 00:12:10,900
+الطبق الذي فيه ما بين ثلاثين إلى ثلاثمائة كولوني هو الذي
+
+160
+00:12:10,900 --> 00:12:16,600
+أختاره، لأنه significant، هو الذي له أهمية.
+
+161
+00:12:16,600 --> 00:12:22,660
+طيب، لو كان عدد الكولونيات أقل من ثلاثين كولوني في
+
+162
+00:12:22,660 --> 00:12:28,060
+الطبق، أقول لك اكتبِ "too few to count"، على طول من حد
+
+163
+00:12:28,060 --> 00:12:31,420
+ما تشوفي عدد الكولونيات في الطبق أقل من ثلاثين،
+
+164
+00:12:31,420 --> 00:12:36,570
+لا تعودي في القانون، اكتبِ "too few to count". طيب، لو
+
+165
+00:12:36,570 --> 00:12:43,610
+كان أكثر من ثلاثمائة، لا تعودي، اكتبِ في النتيجة "too numerous to count".
+
+166
+00:12:43,610 --> 00:12:47,630
+يبقى لو كان أقل من ثلاثين "too few to count"، اختصارًا، أو إذا كان أكثر من
+
+167
+00:12:47,630 --> 00:12:51,650
+ثلاثمائة، أكتب "too numerous to count"، تمام؟ لكن لو كان
+
+168
+00:12:51,650 --> 00:12:59,070
+بين الثلاثين والثلاثمائة، أعدّ هذه الكولونيات، وفي النهاية أعود
+
+169
+00:12:59,070 --> 00:13:03,130
+إلى القانون الذي سنذكره في الشرائح القادمة. هذا الطبق
+
+170
+00:13:07,290 --> 00:13:12,790
+تقريبًا فيه 7 أو 8 كولونيات، بالتالي هو
+
+171
+00:13:12,790 --> 00:13:17,670
+أقل من ثلاثين، فأكتب على طول "too few to count". هو ليس
+
+172
+00:13:17,670 --> 00:13:21,530
+مناسبًا أن أعدّهم. نلاحظ أنه تقريبًا ما بين
+
+173
+00:13:21,530 --> 00:13:25,750
+الثلاثين والثلاثمائة، فأعدّ هذه الكولونيات، كم عددها؟ وفي النهاية
+
+174
+00:13:26,200 --> 00:13:29,680
+أعود لـ number of colonies. فيه قانون سنتعرف عليه.
+
+175
+00:13:29,680 --> 00:13:33,580
+نلاحظ أن هذا الطبق أكثر من ثلاثمائة، تقريبًا،
+
+176
+00:13:33,580 --> 00:13:37,440
+بالتالي يصعب أن أعدّه، فعلى طول لا أعدّه، أكتب "too numerous to count". طيب،
+
+177
+00:13:37,440 --> 00:13:42,240
+نيجي للخطوات، بالنسبة للخطوات، كيف أريد أن أعمل
+
+178
+00:13:42,240 --> 00:13:48,480
+Serial Dilution method أو Plate Count method أو
+
+179
+00:13:48,480 --> 00
+
+223
+00:16:59,920 --> 00:17:03,820
+نعمل نزرع على كل توب على طبقين وناخد الـAverage
+
+224
+00:17:03,820 --> 00:17:08,580
+المتوسط، نأخذ على سبيل المثال 1 من 10 ML من هذا
+
+225
+00:17:08,580 --> 00:17:14,090
+التيوب بحطه على أطباق و بزرع و بفرد و هكذا في كل
+
+226
+00:17:14,090 --> 00:17:19,910
+تيوبات، بعد 24 ساعة بعمل count للـ  .. للـ ..
+
+227
+00:17:19,910 --> 00:17:26,010
+اللي هو الـ .. الأطباق، لكلنا طبعاً هتطلع في عندي هنا
+
+228
+00:17:26,010 --> 00:17:30,610
+في الأغلب أقل تخفيف اللي هي too numerous to count
+
+229
+00:17:30,610 --> 00:17:34,630
+آخر شيء هتكون too few to count لكن هنا تقريباً
+
+230
+00:17:34,630 --> 00:17:37,670
+هيطلع في الـ .. اللي في النص ممكن أن أقدر أعدها
+
+231
+00:17:38,150 --> 00:17:42,570
+فهعيد كل الكلام  و أعوض في القانون اللي هنذكره في
+
+232
+00:17:42,570 --> 00:17:46,070
+الـ slides اللي بعدي، أنا بقول أنك أنت بتنقلي طبعاً
+
+233
+00:17:46,070 --> 00:17:49,590
+1 ML من كل tube بتنقليها للـ tube الثاني و
+
+234
+00:17:49,590 --> 00:17:52,890
+بالنهاية التخفيف هو تخفيفها في تخفيف اللي
+
+235
+00:17:52,890 --> 00:17:59,490
+قبلها، نفس الفكرة، بعدين بتعملي طبعاً بتأخذي من كل
+
+236
+00:17:59,490 --> 00:18:04,250
+tube و بتحطيه على طبق أجار، مكتوب، وبعدين بتعملي
+
+237
+00:18:04,250 --> 00:18:10,040
+اللي هو incubation من 24 لـ 48 ساعة، بعدين بقول لك
+
+238
+00:18:10,040 --> 00:18:13,080
+بتعدي الـ colonies في الأطباق فقط لما يكون عدد الـ
+
+239
+00:18:13,080 --> 00:18:19,620
+colonies من 30 لـ 200 أو لـ 300 colonies لو كان too
+
+240
+00:18:19,620 --> 00:18:23,820
+numerous to count، أكثر من 300 بكتب too numerous to
+
+241
+00:18:23,820 --> 00:18:28,240
+count، لو كان أقل من 30 بكتب too few to count، هذا
+
+242
+00:18:28,240 --> 00:18:33,580
+بالنسبة للطريقة اللي أنا بشتغل عليها في هاي الـ
+
+243
+00:18:33,580 --> 00:18:39,940
+slide، نفس الفكرة لكن الاختلاف أنه هنا أذكر في التيوب
+
+244
+00:18:39,940 --> 00:18:43,580
+مش حاطة 9 ML، احنا قلنا مش شرط أني أنا أحط 9 ML
+
+245
+00:18:43,580 --> 00:18:49,560
+بحط أي حجم بدي إياه، لكن بالنهاية لازم أكون عارف هذا
+
+246
+00:18:49,560 --> 00:18:54,640
+الحجم على أساسه هأحسب الـ dilution، هنا حطيت 4
+
+247
+00:18:54,640 --> 00:18:59,860
+.5 ML من الـ diluent، وأخذ نص ML من اللي هو التيوب
+
+248
+00:18:59,860 --> 00:19:04,340
+يبقى نص على نص، كيف بدي أحسب dilution هاي التيوب؟ نص على نص
+
+249
+00:19:04,340 --> 00:19:08,700
+زائد أربعة ونص بيطلع بالنهاية اللي هو عشرة أس سالب
+
+250
+00:19:08,700 --> 00:19:13,200
+واحد، طيب ليش هنا حطيت عشرة أس سالب اثنين تخفيفها لأن
+
+251
+00:19:13,200 --> 00:19:21,540
+هاي العينة مخففة في المثال اللي قبل، في
+
+252
+00:19:21,540 --> 00:19:25,500
+المثال هذا كانت العينة الأصلية مش مخففة، أخذت واحد
+
+253
+00:19:25,500 --> 00:19:30,960
+ML من الـ high tube وضفتها على الـ diluent، وحسبت الـ
+
+254
+00:19:30,960 --> 00:19:34,480
+dilution بدون ما أضرب بـ dilution لـ هذه العينة لأن
+
+255
+00:19:34,480 --> 00:19:39,980
+هذه العينة مش مخففة، يبقى ننتبه في السؤال: هل العينة
+
+256
+00:19:39,980 --> 00:19:44,340
+الأصلية مخففة؟ إذا مخففة لازم أضرب أول tube في
+
+257
+00:19:44,340 --> 00:19:48,930
+تخفيف الـ high tube، العينة الأصلية، لكن لو مش مخففة
+
+258
+00:19:48,930 --> 00:19:53,590
+خلاص، على طول زي ما ذكرنا في هذا المثال، على طول بعمل
+
+259
+00:19:53,590 --> 00:19:57,070
+اللي هو التخفيف، بحسب التخفيف بدون ما أضرب في
+
+260
+00:19:57,070 --> 00:20:00,350
+dilution الـ high tube، لأنه الـ high tube مش مخففة
+
+261
+00:20:00,350 --> 00:20:07,010
+فهذا المثال هنا العينة high مخففة، و هذا كل تخفيفها
+
+262
+00:20:07,010 --> 00:20:12,310
+عشرة أس سالب واحد، يبقى تخفيف أول tube اللي هو الـ
+
+263
+00:20:12,310 --> 00:20:15,890
+sample اللي أنا أخذته، نص على sample، نص زائد
+
+264
+00:20:15,890 --> 00:20:21,110
+أربعة ونص، يبقى بالنهاية بيطلع نص على خمسة، نص على خمسة
+
+265
+00:20:21,110 --> 00:20:25,650
+واحد على عشرة، عشرة أس سالب واحد، في تخفيف الـ tube اللي
+
+266
+00:20:25,650 --> 00:20:28,330
+قبلها اللي هي عشرة أس سالب واحد، يبقى عشرة أس سالب
+
+267
+00:20:28,330 --> 00:20:32,470
+اثنين، طبعاً هاي الـ tube تخفيفها أربعة، بعمل بنفس
+
+268
+00:20:32,470 --> 00:20:36,350
+الفكرة، نص على نص زائد أربعة ونص، عشرة أس سالب واحد، ضرب
+
+269
+00:20:36,350 --> 00:20:40,230
+تخفيف هاي، عشرة أس سالب اثنين، بالنهاية بيطلع عشرة أس سالب
+
+270
+00:20:40,230 --> 00:20:42,530
+أربعة في هاي الـ tube، عشرة أس سالب خمسة في هاي الـ tube،
+
+271
+00:20:42,530 --> 00:20:48,160
+عشرة أس سالب ستة في هاي الـ tube، آخذ من كل الـ tubes نص
+
+272
+00:20:48,160 --> 00:20:53,640
+أو واحد من عشرة ML وراح أزرعها في طبق، تمام؟ راح
+
+273
+00:20:53,640 --> 00:20:59,060
+أزرعها في هاي الأطباق و أحطها في الـ incubator، يبقى
+
+274
+00:20:59,060 --> 00:21:03,400
+في هذا المثال ننتبه أنه هنا العينة هاي مخففة، فلازم
+
+275
+00:21:03,400 --> 00:21:06,860
+أضرب أول tube في تخفيفها، لكن في المثال اللي أن ذكر
+
+276
+00:21:06,860 --> 00:21:11,540
+في الأول ما كانت العينة مخففة، عشان هيك ما ضربتش، ممكن
+
+277
+00:21:11,540 --> 00:21:16,320
+في مثال ثاني ما يذكر لي اللي هو هنا تخفيفها عشرة أس سالب
+
+278
+00:21:16,320 --> 00:21:23,120
+واحد، ممكن يقول لي والله أخذت خمسة وعشرين ML من
+
+279
+00:21:23,120 --> 00:21:27,740
+الملح وضفتهم على مائتين وخمسة وعشرين من الـ normal
+
+280
+00:21:27,740 --> 00:21:32,060
+saline، فهنا بيدي وقتها أحسب تخفيف اللي هو هاي الـ
+
+281
+00:21:32,060 --> 00:21:36,000
+sample اللي بنحكي عنه، خمسة وعشرين على خمسة وعشرين زائد
+
+282
+00:21:36,000 --> 00:21:38,620
+مائتين وخمسة وعشرين، اللي هو الـ sample زائد الـ diluent
+
+283
+00:21:38,620 --> 00:21:43,860
+فبيطلع خمسة وعشرين على مائتين وخمسين، 25 على
+
+284
+00:21:43,860 --> 00:21:48,340
+250 اللي هي 1 على 10، اللي هي 10 أس سالب واحد، هيك
+
+285
+00:21:48,340 --> 00:21:54,040
+بكون حسبت تخفيف هاي التيوب، و بكمل الحل زي ما كملنا
+
+286
+00:21:54,040 --> 00:21:58,960
+يبقى ممكن ما يكون مخفف العينة، ممكن يكون مخففها و
+
+287
+00:21:58,960 --> 00:22:04,200
+يذكر لك التخفيف، ممكن يكون فيه تخفيف لكن مش ذاكر شو
+
+288
+00:22:04,200 --> 00:22:07,620
+قيمة الـ dilution، فأنت بدك تحسب قيمة الـ dilution من
+
+289
+00:22:07,620 --> 00:22:13,400
+القانون: sample على sample زائد diluent، هذا بالنسبة
+
+290
+00:22:13,400 --> 00:22:18,700
+لهذه الصورة، هذا السؤال، هذه الفكرة نفس اللي احنا
+
+291
+00:22:18,700 --> 00:22:22,060
+ذكرناها في الأول، أخذ 1 ML، وضفنا، قلنا كل واحد
+
+292
+00:22:22,060 --> 00:22:25,180
+قد ايش الـ dilution؟ هي ذكرهم: عشرة أس سالب واحد، عشرة
+
+293
+00:22:25,180 --> 00:22:27,860
+أس سالب اثنين، عشرة أس سالب ثلاثة، عشرة أس سالب أربعة،
+
+294
+00:22:27,860 --> 00:22:33,400
+عشرة أس سالب خمسة، عشرة أس سالب ستة، واحد من عشرة ML
+
+295
+00:22:33,400 --> 00:22:42,840
+منها، لليتوب، وراح أزرعهم في اللي هو الأطباق
+
+296
+00:22:42,840 --> 00:22:51,120
+وبعد 24 ساعة شاف الـ colonies، طيب
+
+297
+00:22:51,120 --> 00:22:56,430
+احنا قلنا بعد 24 ساعة بحسب، أو بعد الـ colonies إذا كان
+
+298
+00:22:56,430 --> 00:22:59,990
+الأعداد ما بين ثلاثين لتلاثمئة colony forming unit
+
+299
+00:22:59,990 --> 00:23:03,390
+ومنعد بالقانون، شو هو القانون اللي بنعود عليه؟
+
+300
+00:23:03,390 --> 00:23:07,010
+بيقول لي: calculate the number of bacteria colony
+
+301
+00:23:07,010 --> 00:23:11,890
+forming unit per milliliter or gram of sample by
+
+302
+00:23:11,890 --> 00:23:14,930
+dividing the number of colonies، كيف بدي أعد أو بدي
+
+303
+00:23:14,930 --> 00:23:18,690
+أحسب؟ احنا قلنا بالنهاية، الأعداد count بعبر عنها بـ
+
+304
+00:23:18,690 --> 00:23:23,940
+colony forming unit per ml or gram، تمام؟ طيب كيف
+
+305
+00:23:23,940 --> 00:23:27,620
+بالنهاية بدي أحسب هاي الـ .. الـ measures، أو كيف بدي
+
+306
+00:23:27,620 --> 00:23:31,780
+أعبر بـ Colony Forming Unit per ml or gram؟ بيقول لي
+
+307
+00:23:31,780 --> 00:23:35,980
+بتقسمي الـ number of colonies، عدد الـ colonies اللي
+
+308
+00:23:35,980 --> 00:23:40,820
+أنتِ عدّيتيه على الطبق اللي كان ما بين 30 لـ 300،
+
+309
+00:23:40,820 --> 00:23:44,820
+قلنا ما بنعود في القانون إذا كان أقل من 30، وإذا
+
+310
+00:23:44,820 --> 00:23:52,430
+أكثر من 300، بعد كده بحط على الـ sample، شو هذا الـ S؟
+
+311
+00:23:52,430 --> 00:23:57,030
+رمز الـ S بيقول لي هو كمية العينة اللي أنتِ أخذتيها
+
+312
+00:23:57,030 --> 00:24:02,810
+من الـ tube، يعني في هذا الـ tube أنا رحت
+
+313
+00:24:02,810 --> 00:24:06,750
+أخذت من هذا الـ tube واحد من عشرة ML وحطيته في
+
+314
+00:24:06,750 --> 00:24:10,130
+الطبق، ورحت فردته و زرعت، قد ايش أنا أخذت من الـ
+
+315
+00:24:10,130 --> 00:24:17,340
+tube هذا؟ بعوض، يبقى هنا واحد من عشرة هي الـ S، واحد
+
+316
+00:24:17,340 --> 00:24:20,380
+من عشرة، يبقى الـ number of colonies، عدد الـ colonies
+
+317
+00:24:20,380 --> 00:24:24,980
+اللي عدّيتها في الطبق،  على قد ايش أنا أخذت من
+
+318
+00:24:24,980 --> 00:24:30,140
+التخفيف ورحت زرعتها على الطبق، الـ D اللي هي الـ
+
+319
+00:24:30,140 --> 00:24:34,020
+dilution، شو الـ tube اللي أنا أخذت منه؟ قد ايش كان الـ
+
+320
+00:24:34,020 --> 00:24:37,540
+dilution؟ قد ايش كان التخفيف في هذه الـ tube؟ أيوا،
+
+321
+00:24:37,540 --> 00:24:41,340
+number of colonies، دي، dilution، blank، الـ S هي
+
+322
+00:24:41,340 --> 00:24:45,280
+volume transferred to plate، قد ايش حجم العينة اللي
+
+323
+00:24:45,280 --> 00:24:48,980
+أنا أخذتها من الـ tube في هذا الـ dilution وحطيته
+
+324
+00:24:48,980 --> 00:24:51,860
+على الـ plate، بالنهاية هأعبّر عن كلها بـ uniform units
+
+325
+00:24:51,860 --> 00:25:00,360
+per ml or gram، طيب قبل ما نذكر هذا المثال في
+
+326
+00:25:00,360 --> 00:25:07,450
+الخطوات، في الخطوات، في هذا المثال ذاكر مثلاً أنه أخذ
+
+327
+00:25:07,450 --> 00:25:13,750
+1 ML من العينة وحطينا 9 ML من الـ diluent، طيب ممكن
+
+328
+00:25:13,750 --> 00:25:22,270
+ما يقول لي أنه قد ايش أنا مثلاً، اللي هو قد ايش أنا أخذت
+
+329
+00:25:22,270 --> 00:25:26,650
+الـ diluent، ممكن ما يكون ذاكر قد ايش رقم الـ diluent،
+
+330
+00:25:26,650 --> 00:25:30,690
+قد ايش اللي هو 9 ML، هاي مش ذاكرها، أو مش ذاكر الـ 1 ML،
+
+331
+00:25:31,540 --> 00:25:37,280
+عشان نروح يقول لي الـ dilution في التوب هاي واحد على
+
+332
+00:25:37,280 --> 00:25:43,440
+عشرة، تمام؟ الـ dilution في هاي التيوب واحد على عشرة،
+
+333
+00:25:43,440 --> 00:25:49,800
+وفي هاي التيوب ضفتِ أنتِ أربعة ونص diluent، قد ايش الـ
+
+334
+00:25:49,800 --> 00:25:54,640
+volume of sample اللي أخذتي؟ يبقى ممكن يسألني سؤال
+
+335
+00:25:54,640 --> 00:25:59,810
+كيف بنحلّ الأسئلة؟ هنا يقول لي: أن في التيوب أنا
+
+336
+00:25:59,810 --> 00:26:07,110
+أخذت 4.5 ML diluent، والتخفيف 1 على 10 أو 10 أس سالب
+
+337
+00:26:07,110 --> 00:26:11,730
+1، بيقول لي: الـ volume of sample قد ايش؟ كيف بدي أحسب الـ
+
+338
+00:26:11,730 --> 00:26:16,450
+volume of sample؟ يعني اللي هي هنا هادي، قد ايش هي؟ مش
+
+339
+00:26:16,450 --> 00:26:21,030
+ذاكرها، نيجي عشان تكون الصورة واضحة، اللي هو الـ
+
+340
+00:26:21,030 --> 00:26:21,390
+diluent
+
+341
+00:26:33,560 --> 00:26:37,800
+في tube أربعة
+
+342
+00:26:37,800 --> 00:26:41,920
+و نص ML
+
+343
+00:26:41,920 --> 00:26:50,800
+الـ dilution اللي
+
+344
+00:26:50,800 --> 00:26:56,480
+هو واحد على، بيقول لي
+
+345
+00:26:56,480 --> 00:27:02,080
+الـ volume of
+
+346
+00:27:03,240 --> 00:27:11,680
+sample، أنت بدك تجيبي قد ايش هو؟ كيف أنا بدي أجيبّه هلأ؟
+
+347
+00:27:11,680 --> 00:27:15,400
+بدنا نعرف أنّه أنا الـ dilution، اش هو الـ dilution؟ الـ
+
+348
+00:27:15,400 --> 00:27:22,840
+dilution: sample على sample
+
+349
+00:27:22,840 --> 00:27:27,660
+الـ dilution
+
+350
+00:27:33,960 --> 00:27:37,860
+التخفيف، الـ dilution، شو هو الـ dilution؟ الـ dilution
+
+351
+00:27:37,860 --> 00:27:42,180
+هو الـ volume of sample على الـ volume of sample
+
+352
+00:27:42,180 --> 00:27:46,780
+زائد الـ volume of diluent، طبعاً، طيب الـ volume of
+
+353
+00:27:46,780 --> 00:27:50,220
+هذا هو بالنسبة للـ dilution، طب الـ dilution
+
+354
+00:27:50,220 --> 00:27:55,800
+بيعطيني اللي هو واحد على عشرة، يبقى هنا واحد على
+
+355
+00:27:55,800 --> 00:28:05,100
+عشرة، واحد على عشرة، طيب هلأ الـ sample هي حتكون قد ايش؟
+
+356
+00:28:05,100 --> 00:28:11,400
+الـ sample هي اللي هو أو التخفيف اللي هو واحد على
+
+357
+00:28:11,400 --> 00:28:16,600
+واحد على عشرة، هذا هو الـ total، يبقى الـ total هو
+
+358
+00:28:16,600 --> 00:28:21,680
+العشرة، الـ sample زائد الـ diluent هو الـ total اللي
+
+359
+00:28:21,680 --> 00:28:26,120
+هو عشرة، من هذا ممكن أني أنا أقدر قد ايش أجيب الـ
+
+360
+00:28:26,120 --> 00:28:31,220
+volume of sample، يبقى الـ total هيساوي
+
+361
+00:28:32,840 --> 00:28:39,520
+اللي هو الـ sample زائد الـ diluent، الـ total بيساوي
+
+362
+00:28:39,520 --> 00:28:46,000
+الـ sample زائد الـ diluent ويساوي عشرة
+
+363
+00:28:46,000 --> 00:28:50,340
+من هذا، اللي هو من خلال الـ dilution واحد على
+
+364
+00:28:50,340 --> 00:28:54,540
+عشرة، يبقى الـ total هو عشرة، طب قال لي أن الـ diluent
+
+365
+00:28:54,540 --> 00:28:58,580
+في الـ tube أربعة ونص، يبقى الـ diluent موجودة هنا
+
+366
+00:28:58,580 --> 00:29:04,780
+أعوض في القانون هنا، الـ sample مش معروفة، أنا بدي
+
+367
+00:29:04,780 --> 00:29:13,460
+أجيبها، زائد الـ diluent أربعة ونص، والـ total من الـ
+
+368
+00:29:13,460 --> 00:29:17,020
+dilution جبناها عشرة، طب قد ايش الـ sample؟ اللي هي الـ
+
+369
+00:29:17,020 --> 00:29:22,640
+sample حتساوي خمسة، يبقى
+
+370
+00:29:22,640 --> 00:29:26,240
+الـ sample حتكون خمسة، هيك أنا جيبت الـ sample، ممكن ما
+
+371
+00:29:26,240 --> 00:29:31,060
+يكون مديني إياها في القانون أو في المسألة، فأنا بدي
+
+372
+00:29:31,060 --> 00:29:34,880
+أجيب الـ sample، نفس الفكرة ممكن يجيب لي الـ sample لكن
+
+373
+00:29:34,880 --> 00:29:38,280
+ما يجيب لي الـ diluent، يقول لي الـ sample اللي أنا أخذتها
+
+374
+00:29:38,280 --> 00:29:42,580
+من أول tube اللي هي نص ML، الـ dilution واحد من عشرة،
+
+375
+00:29:42,580 --> 00:29:46,660
+قد ايش الـ volume of diluent؟ هأعوض بنفس القانون: sample
+
+376
+00:29:46,660 --> 00:29:50,680
+على sample زائد diluent، واحد على عشرة، التعويض اللي
+
+377
+00:29:50,680 --> 00:29:53,700
+هي الـ sample زائد الـ diluent عشرة، الـ sample
+
+378
+00:29:53,700 --> 00:29:57,440
+قد ايش إياها؟ اللي هي نص، زائد الـ diluent مش مديني إياها،
+
+379
+00:29:57,440 --> 00:30:02,640
+ويساوي عشرة، هأحسب بالنهاية، هيطلع الـ diluent تسعة، هيك
+
+380
+00:30:02,640 --> 00:30:07,700
+أنا ممكن، يبقى في المسألة ما يجيب لي الـ diluent،
+
+381
+00:30:07,700 --> 00:30:10,560
+ممكن ما يجيب لي الـ dilution، ممكن ما يجيب لي الـ
+
+382
+00:30:10,560 --> 00:30:15,960
+volume of sample، كلهم بنفس الفكرة من خلال القانون
+
+383
+00:30:15,960 --> 00:30:19,840
+sample على sample زائد diluent، هو هذا الـ dilution،
+
+384
+00:30:19,840 --> 00:30:23,820
+من خلاله أنا بقدر أجيب أي واحد منهم وأكمل المسألة
+
+385
+00:30:23,820 --> 00:30:28,920
+وأحلها بشكل بسيط وسهل، قلنا ممكن العينة الأصلية
+
+386
+00:30:28,920 --> 00:30:32,640
+مباشرة آخذ منها، فبالتالي مش هأخفف الـ tube أو الـ
+
+387
+00:30:32,640 --> 00:30:37,370
+tubes، لو العينة الأصلية مخففة زي ما ذكرنا في مثال
+
+388
+00:30:37,370 --> 00:30:40,810
+اللي كان مكتوب فيه عشرة أس سالب واحد تخفيفها، لازم
+
+389
+00:30:40,810 --> 00:30:4
+
+445
+00:34:53,670 --> 00:34:58,170
+يجيبلك عدد الكولوني أقل من ثلاثين أو أكثر من
+
+446
+00:34:58,170 --> 00:35:02,170
+ثلاثمية فبالتالي هنلجأ للتعويض في القانون هنكتب
+
+447
+00:35:02,170 --> 00:35:07,410
+too few to count or too numerous to count هيكون
+
+448
+00:35:07,410 --> 00:35:11,510
+خلصنا  الشرح يعطيكم العافية والسلام عليكم ورحمة
+
+449
+00:35:11,510 --> 00:35:12,570
+الله وبركاته
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ssK-XRx65XI_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ssK-XRx65XI_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..d32e77414f46c21c0fbd2341b8585c2925000e05
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/ssK-XRx65XI_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 3074, "start": 1.62, "end": 30.74, "text": "فنبدأ بالجزء التاني من الـ Chapter اللي هو بتكلم عن الـ Microbial Control Agent عوامل التحكم بالميكروبات Many microorganisms are beneficial and necessary for human wellbeing قولنا إن نسبة كبيرة من الميكروبات تعتبر beneficial، مفيدة ونسبة قليلة منها تعتبر non-pathogenic however, microbial activities may have undesirable", "tokens": [5172, 1863, 44510, 10721, 20666, 7435, 11622, 38207, 16712, 7649, 1829, 9154, 2423, 39184, 18874, 13672, 1829, 31439, 39894, 4117, 19528, 18871, 2423, 39184, 5818, 16614, 831, 12912, 27174, 6225, 2407, 10943, 1211, 16712, 5016, 24793, 20666, 2304, 1829, 4117, 32887, 3555, 9307, 5126, 49129, 13539, 366, 14072, 293, 4818, 337, 1952, 29508, 12174, 12610, 8315, 36145, 8717, 35457, 3660, 9122, 3555, 48923, 9154, 9673, 1829, 4117, 32887, 3555, 9307, 6055, 34268, 26890, 14072, 12399, 3714, 5172, 25708, 3660, 4032, 1863, 35457, 3660, 12174, 20292, 37977, 9154, 11296, 6055, 34268, 26890, 2107, 12, 31852, 25473, 4461, 11, 49713, 831, 5354, 815, 362, 45667, 21493], "avg_logprob": -0.14620535033089774, "compression_ratio": 1.5261324041811846, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 1.62, "end": 2.14, "word": "فنبدأ", "probability": 0.9189453125}, {"start": 2.14, "end": 2.78, "word": " بالجزء", "probability": 0.9786376953125}, {"start": 2.78, "end": 3.2, "word": " التاني", "probability": 0.9599609375}, {"start": 3.2, "end": 3.46, "word": " من", "probability": 0.994140625}, {"start": 3.46, "end": 3.7, "word": " الـ", "probability": 0.81396484375}, {"start": 3.7, "end": 4.28, "word": " Chapter", "probability": 0.63671875}, {"start": 4.28, "end": 4.98, "word": " اللي", "probability": 0.916259765625}, {"start": 4.98, "end": 5.26, "word": " هو", "probability": 0.96435546875}, {"start": 5.26, "end": 5.78, "word": " بتكلم", "probability": 0.8219401041666666}, {"start": 5.78, "end": 6.14, "word": " عن", "probability": 0.99658203125}, {"start": 6.14, "end": 6.52, "word": " الـ", "probability": 0.7890625}, {"start": 6.52, "end": 7.18, "word": " Microbial", "probability": 0.7674153645833334}, {"start": 7.18, "end": 7.88, "word": " Control", "probability": 0.7783203125}, {"start": 7.88, "end": 8.46, "word": " Agent", "probability": 0.69091796875}, {"start": 8.46, "end": 9.12, "word": " عوامل", "probability": 0.98681640625}, {"start": 9.12, "end": 9.82, "word": " التحكم", "probability": 0.9925130208333334}, {"start": 9.82, "end": 11.12, "word": " بالميكروبات", "probability": 0.7925153459821429}, {"start": 11.12, "end": 12.02, "word": " Many", "probability": 0.254150390625}, {"start": 12.02, "end": 13.12, "word": " microorganisms", "probability": 0.877685546875}, {"start": 13.12, "end": 13.5, "word": " are", "probability": 0.96044921875}, {"start": 13.5, "end": 14.08, "word": " beneficial", "probability": 0.8974609375}, {"start": 14.08, "end": 14.56, "word": " and", "probability": 0.88525390625}, {"start": 14.56, "end": 15.1, "word": " 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1680.78, "word": " بالنسبة", "probability": 0.9517822265625}, {"start": 1680.78, "end": 1681.32, "word": " للرقم", "probability": 0.891845703125}, {"start": 1681.32, "end": 1681.52, "word": " ليش", "probability": 0.6619873046875}, {"start": 1681.52, "end": 1681.66, "word": " هان", "probability": 0.49560546875}, {"start": 1681.66, "end": 1681.96, "word": " مكتوب", "probability": 0.9827880859375}, {"start": 1681.96, "end": 1682.26, "word": " عشرة", "probability": 0.92431640625}, {"start": 1682.26, "end": 1682.66, "word": " أسالب", "probability": 0.9832763671875}, {"start": 1682.66, "end": 1683.06, "word": " تلاتة", "probability": 0.986083984375}, {"start": 1683.06, "end": 1683.4, "word": " هي", "probability": 0.438720703125}, {"start": 1683.4, "end": 1684.22, "word": " الرقم", "probability": 0.9759114583333334}, {"start": 1684.22, "end": 1684.5, "word": " هذا", "probability": 0.92919921875}, {"start": 1684.5, "end": 1685.06, "word": " حاصل", "probability": 0.96728515625}, {"start": 1685.06, "end": 1685.36, "word": " ضرب", "probability": 0.995849609375}, {"start": 1685.36, "end": 1685.6, "word": " ال", "probability": 0.68994140625}, {"start": 1685.6, "end": 1686.14, "word": " dilution", "probability": 0.55975341796875}, {"start": 1686.14, "end": 1686.48, "word": " مع", "probability": 0.9755859375}, {"start": 1686.48, "end": 1686.72, "word": " ال", "probability": 0.947265625}, {"start": 1686.72, "end": 1686.92, "word": " S", "probability": 0.5810546875}, {"start": 1686.92, "end": 1687.16, "word": " هان", "probability": 0.783935546875}, {"start": 1687.16, "end": 1687.6, "word": " أخدت", "probability": 0.9515380859375}, {"start": 1687.6, "end": 1687.94, "word": " واحد", "probability": 0.99169921875}, {"start": 1687.94, "end": 1688.12, "word": " من", "probability": 0.9921875}, {"start": 1688.12, "end": 1688.44, "word": " عشرة", "probability": 0.9803059895833334}, {"start": 1688.44, "end": 1688.74, "word": " لكن", "probability": 0.880859375}, {"start": 1688.74, "end": 1689.12, "word": " هان", "probability": 0.912109375}, {"start": 1689.12, "end": 1689.98, "word": " أخدت", "probability": 0.94287109375}, {"start": 1689.98, "end": 1690.5, "word": " واحد", "probability": 0.9912109375}, {"start": 1690.5, "end": 1691.16, "word": " ML", "probability": 0.95947265625}, {"start": 1691.16, "end": 1692.3, "word": " لازم", "probability": 0.9767252604166666}, {"start": 1692.3, "end": 1692.98, "word": " يكون", "probability": 0.9921875}, {"start": 1692.98, "end": 1694.68, "word": " الرقم", "probability": 0.9436848958333334}, {"start": 1694.68, "end": 1695.08, "word": " اللي", "probability": 0.97216796875}, {"start": 1695.08, "end": 1695.96, "word": " هو", "probability": 0.85400390625}, {"start": 1695.96, "end": 1696.14, "word": " ال", "probability": 0.984375}, {"start": 1696.14, "end": 1696.36, "word": " S", "probability": 0.9072265625}, {"start": 1696.36, "end": 1696.68, "word": " لازم", "probability": 0.9860026041666666}, {"start": 1696.68, "end": 1697.06, "word": " يكون", "probability": 0.992431640625}, {"start": 1697.06, "end": 1697.28, "word": " بال", "probability": 0.9296875}, {"start": 1697.28, "end": 1697.68, "word": " ML", "probability": 0.521484375}, {"start": 1697.68, "end": 1697.96, "word": " لو", "probability": 0.95068359375}, {"start": 1697.96, "end": 1698.26, "word": " كان", "probability": 0.9892578125}, {"start": 1698.26, "end": 1698.46, "word": " في", "probability": 0.97216796875}, {"start": 1698.46, "end": 1698.62, "word": " ال", "probability": 0.8271484375}, {"start": 1698.62, "end": 1699.04, "word": " micro", "probability": 0.8720703125}], "temperature": 1.0}, {"id": 63, "seek": 172640, "start": 1699.72, "end": 1726.4, "text": "لازم أحاوله لـ ML هذه معلومة مهمة لازم ننتبه لإلها في المثاقات هيك بنكون وصلنا لنهاية شرح المحاضرة وأيضا لنهاية سلسلة محاضرات مساق الـ Essential Medical Microbiology بتمنعكم كل التوفيق والنجاح والسلام عليكم ورحمة الله وبركاته", "tokens": [1211, 31377, 2304, 5551, 5016, 995, 12610, 3224, 5296, 39184, 21601, 29538, 20449, 1211, 20498, 3660, 3714, 16095, 3660, 5296, 31377, 2304, 8717, 29399, 3555, 3224, 5296, 28814, 1211, 11296, 8978, 9673, 12984, 995, 4587, 9307, 39896, 4117, 44945, 30544, 4032, 36520, 8315, 5296, 1863, 11296, 10632, 13412, 2288, 5016, 9673, 5016, 46958, 25720, 36725, 1829, 11242, 995, 5296, 1863, 11296, 10632, 8608, 1211, 3794, 37977, 3714, 5016, 46958, 2288, 9307, 47524, 995, 4587, 2423, 39184, 49736, 15896, 5818, 20027, 1793, 39894, 27842, 3615, 24793, 28242, 16712, 38688, 1829, 4587, 16070, 1863, 7435, 39319, 16070, 3794, 37440, 25894, 24793, 4032, 2288, 35571, 3660, 21984, 4032, 26890, 4117, 9307, 3224], "avg_logprob": -0.13124999864534898, "compression_ratio": 1.63135593220339, "no_speech_prob": 6.461143493652344e-05, "words": [{"start": 1699.72, "end": 1700.18, "word": "لازم", "probability": 0.9029947916666666}, {"start": 1700.18, "end": 1700.74, "word": " أحاوله", "probability": 0.794775390625}, {"start": 1700.74, "end": 1701.14, "word": " لـ", "probability": 0.6676025390625}, {"start": 1701.14, "end": 1701.58, "word": " ML", "probability": 0.8056640625}, {"start": 1701.58, "end": 1702.1, "word": " هذه", "probability": 0.30322265625}, {"start": 1702.1, "end": 1702.76, "word": " معلومة", "probability": 0.9771728515625}, {"start": 1702.76, "end": 1703.22, "word": " مهمة", "probability": 0.9879557291666666}, {"start": 1703.22, "end": 1703.48, "word": " لازم", "probability": 0.98388671875}, {"start": 1703.48, "end": 1703.86, "word": " ننتبه", "probability": 0.9521484375}, {"start": 1703.86, "end": 1704.26, "word": " لإلها", "probability": 0.876708984375}, {"start": 1704.26, "end": 1704.52, "word": " في", "probability": 0.978515625}, {"start": 1704.52, "end": 1706.24, "word": " المثاقات", "probability": 0.712841796875}, {"start": 1706.24, "end": 1710.4, "word": " هيك", "probability": 0.66162109375}, {"start": 1710.4, "end": 1710.76, "word": " بنكون", "probability": 0.816650390625}, {"start": 1710.76, "end": 1711.3, "word": " وصلنا", "probability": 0.9656575520833334}, {"start": 1711.3, "end": 1712.22, "word": " لنهاية", "probability": 0.95654296875}, {"start": 1712.22, "end": 1712.72, "word": " شرح", "probability": 0.9939778645833334}, {"start": 1712.72, "end": 1713.52, "word": " المحاضرة", "probability": 0.8994140625}, {"start": 1713.52, "end": 1714.2, "word": " وأيضا", "probability": 0.83123779296875}, {"start": 1714.2, "end": 1715.14, "word": " لنهاية", "probability": 0.9378662109375}, {"start": 1715.14, "end": 1715.68, "word": " سلسلة", "probability": 0.995849609375}, {"start": 1715.68, "end": 1716.54, "word": " محاضرات", "probability": 0.9650390625}, {"start": 1716.54, "end": 1717.2, "word": " مساق", "probability": 0.9000651041666666}, {"start": 1717.2, "end": 1717.74, "word": " الـ", "probability": 0.936767578125}, {"start": 1717.74, "end": 1718.16, "word": " Essential", "probability": 0.57568359375}, {"start": 1718.16, "end": 1718.88, "word": " Medical", "probability": 0.80322265625}, {"start": 1718.88, "end": 1720.04, "word": " Microbiology", "probability": 0.9423828125}, {"start": 1720.04, "end": 1721.5, "word": " بتمنعكم", "probability": 0.72076416015625}, {"start": 1721.5, "end": 1721.86, "word": " كل", "probability": 0.990234375}, {"start": 1721.86, "end": 1722.48, "word": " التوفيق", "probability": 0.981689453125}, {"start": 1722.48, "end": 1723.22, "word": " والنجاح", "probability": 0.931640625}, {"start": 1723.22, "end": 1724.12, "word": " والسلام", "probability": 0.9440104166666666}, {"start": 1724.12, "end": 1724.68, "word": " عليكم", "probability": 0.96337890625}, {"start": 1724.68, "end": 1725.18, "word": " ورحمة", "probability": 0.970458984375}, {"start": 1725.18, "end": 1725.5, "word": " الله", "probability": 0.9716796875}, {"start": 1725.5, "end": 1726.4, "word": " وبركاته", "probability": 0.96845703125}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 1727.8200625, "duration_after_vad": 1691.157499999995}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/wyOj5_y9CBQ.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/wyOj5_y9CBQ.srt
new file mode 100644
index 0000000000000000000000000000000000000000..1c28ce5e21a75953c5256d92f5869081842cba3a
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/wyOj5_y9CBQ.srt
@@ -0,0 +1,866 @@
+1
+00:00:00,880 --> 00:00:04,040
+بدنا نحكي في هذه الـ slide عن قواعد السلامة  
+
+2
+00:00:04,040 --> 00:00:08,300
+وإجراءات السلامة والوقاية داخل معمل الـ 
+
+3
+00:00:08,300 --> 00:00:11,440
+microbiology in the microbiology laboratory
+
+4
+00:00:11,440 --> 00:00:14,480
+infectious materials are processed as though they were
+
+5
+00:00:14,480 --> 00:00:17,480
+the microorganisms used in this course may be
+
+6
+00:00:17,480 --> 00:00:20,960
+pathogenic to humans في معمل ال microbiology
+
+7
+00:00:20,960 --> 00:00:25,480
+بدنا نتعامل مع أي شيء كأنه infectious كأنه معدي
+
+8
+00:00:25,480 --> 00:00:29,300
+وممكن يسبب لي مرض كأنه pathogenic to humans ممكن
+
+9
+00:00:29,300 --> 00:00:33,600
+أنه يسبب مرض للإنسان، احنا هنتعامل مع بكتيريا
+
+10
+00:00:33,600 --> 00:00:37,660
+هنزرع بكتيريا، هنجري فحوصات على بكتيريا، هتزرعي
+
+11
+00:00:37,660 --> 00:00:42,420
+بكتيريا، هتمسكي طبق فيه مزروع عليه بكتيريا، عشان هيك
+
+12
+00:00:42,420 --> 00:00:46,900
+أي طبق احنا بنعطيكيه اعتبريه أنه infectious، أنه
+
+13
+00:00:46,900 --> 00:00:51,360
+معدي وممكن يسبب لك مرض. Also, the materials found
+
+14
+00:00:51,940 --> 00:00:55,740
+برضه الأدوات الموجودة في المعمل، such as glassware
+
+15
+00:00:55,740 --> 00:00:59,340
+equipment زي زجاجيات: beakers, flasks, cylinders,
+
+16
+00:00:59,500 --> 00:01:03,460
+tubes, biological agents and chemical المواد
+
+17
+00:01:03,460 --> 00:01:06,500
+الكيميائية برضه بدي أتعامل معها كإنها infectious،
+
+18
+00:01:06,500 --> 00:01:10,920
+كإنها معديّة وممكن إنها تسبب لي مرض. Campos safety
+
+19
+00:01:10,920 --> 00:01:15,640
+hazard، ممكن إنها تشكل خطر على حياتنا. If you don't
+
+20
+00:01:15,640 --> 00:01:18,960
+follow laboratory protocol، إذا ما اتبعنا قواعد
+
+21
+00:01:18,960 --> 00:01:24,940
+السلامة والأمان داخل معمل الـ microbiology. As a
+
+22
+00:01:24,940 --> 00:01:28,920
+result، لذا، certain rules، هناك قواعد، are
+
+23
+00:01:28,920 --> 00:01:31,680
+necessary to prevent the spread of infectious
+
+24
+00:01:31,680 --> 00:01:37,340
+agents. ضروري أن نلتزم بها حتى نمنع انتشار العدوى
+
+25
+00:01:37,340 --> 00:01:41,500
+The possibility of infection of yourselves or other
+
+26
+00:01:41,500 --> 00:01:46,540
+people، وحتى أُقلل فرصة احتمالية إصابة نفسي أو أحد
+
+27
+00:01:46,540 --> 00:01:50,440
+قريب مني بالعدوى. كيف ممكن أحد قريب مني بالعدوى
+
+28
+00:01:50,440 --> 00:01:54,860
+ينصاب؟ روحتي أنتِ على سبيل المثال، زرعتِ بكتيريا، بعد
+
+29
+00:01:54,860 --> 00:01:59,460
+ما زرعتِ، حطيتِ يدكِ على الـ bench، طبعاً هيك الـ bench
+
+30
+00:01:59,460 --> 00:02:03,920
+بيصير ملوّث بالبكتيريا. اجت صحبتك، حطّت قلم على الـ
+
+31
+00:02:03,920 --> 00:02:08,060
+bench، خلصت المعمل، أخذت القلم وحطّته بالشنطة، بعد ما
+
+32
+00:02:08,060 --> 00:02:11,840
+روحت على البيت، اجى أخوها الصغير وحطّ هذا القلم في
+
+33
+00:02:11,840 --> 00:02:17,120
+فمه، هان أخوها الصغير اتعرّض لعدوى نتيجة عدم
+
+34
+00:02:17,120 --> 00:02:20,540
+اتباع إجراءات السلامة والوقاية في معمل الـ
+
+35
+00:02:20,540 --> 00:02:24,900
+Microbiology. عشان هيك لازم نتبع هذه الإجراءات حتى
+
+36
+00:02:24,900 --> 00:02:31,630
+نحمي أنفسنا ونحمي كل قريبنا من العدوى. برضه الهاتف
+
+37
+00:02:31,630 --> 00:02:35,250
+ممنوع نمسكه في معمل الـ Microbiology. أي أدوات خلال
+
+38
+00:02:35,250 --> 00:02:39,430
+الشغل أنا بدي استخدمها، ضروري بعد ما أنا، قبل ما
+
+39
+00:02:39,430 --> 00:02:46,810
+أطلع من معمل الـ Microbiology، إني أنعقّمها. All
+
+40
+00:02:46,810 --> 00:02:50,070
+healthcare workers should routinely use
+
+41
+00:02:50,070 --> 00:02:54,410
+appropriate barrier precautions. قلنا كل العاملين في
+
+42
+00:02:54,410 --> 00:03:01,130
+المجال الصحي لازم يستخدموا بشكل روتيني precautions،
+
+43
+00:03:01,130 --> 00:03:05,170
+يعني وسائل حماية مناسبة، لازم يستخدموا وسائل حماية
+
+44
+00:03:05,170 --> 00:03:10,770
+مناسبة to prevent skin and mucous
+
+45
+00:03:10,770 --> 00:03:16,130
+membrane exposure من أنه تتعرض
+
+46
+00:03:16,130 --> 00:03:20,490
+when in contact with blood or other body fluids of any
+
+47
+00:03:20,490 --> 00:03:25,230
+patient، حتى أنه تتعرض لهذا الـ pathogen أو
+
+48
+00:03:25,230 --> 00:03:29,490
+البكتيريا من العينات المختلفة، سواء من blood أو من
+
+49
+00:03:29,720 --> 00:03:33,800
+سوائل الجسم المختلفة. طيب كيف العاملين في المجال
+
+50
+00:03:33,800 --> 00:03:37,880
+الصحي بيحموا جلدهم؟ بيلبسوا من وسائل الحماية أنهم
+
+51
+00:03:37,880 --> 00:03:41,700
+يلبسوا gloves and to protect mucous membranes. كيف بدي أحمي
+
+52
+00:03:41,700 --> 00:03:46,240
+الأغشية المخاطية؟ إني أنا ألبس الـ mask. تمام. بالنسبة
+
+53
+00:03:46,240 --> 00:03:50,100
+لقواعد السلامة، أهم شيء إني أنا أبدأ بحماية نفسي
+
+54
+00:03:50,100 --> 00:03:55,600
+لأنه لو حاميت نفسي أكيد هحمي الآخرين من العدوى. أول
+
+55
+00:03:55,600 --> 00:04:00,780
+نقطة هي للناس اللي برا، لأنّها، احنا محجّبين للناس اللي
+
+56
+00:04:00,780 --> 00:04:05,400
+بشعرهم، بقول لك لازم إنك أنتِ تحافظي على شعرك يكون
+
+57
+00:04:05,400 --> 00:04:10,880
+مربوط، لأنه ممكن خلال شغلك، إذا كان مش مربوط،
+
+58
+00:04:10,880 --> 00:04:15,500
+الشعر ينزل على الطبق البكتيري. Always wearing
+
+59
+00:04:15,500 --> 00:04:20,980
+closed shoes. في معمل الـ microbiology لازم الـ shoes
+
+60
+00:04:20,980 --> 00:04:25,320
+يكون closed، ممنوع نلبس صندل، لأنه ممكن يكون في
+
+61
+00:04:25,320 --> 00:04:32,090
+رجلكِ يكون فيه جرح. روحتي أنتِ زرعتِ بكتيريا، طبعاً خلال
+
+62
+00:04:32,090 --> 00:04:35,790
+الزراعة مثلاً أنا بستخدم فيه أداة اسمها الـ loop، في
+
+63
+00:04:35,790 --> 00:04:40,990
+الزراعة، وقعت هذه الـ loop على الأرض واجت على رجلكِ
+
+64
+00:04:40,990 --> 00:04:44,210
+اللي فيها الجروح، طبعاً الـ loop فيها بكتيريا، هذه
+
+65
+00:04:44,210 --> 00:04:48,410
+البكتيريا هتدخل من هذا الجرح للجسم وتعمل لكِ
+
+66
+00:04:48,410 --> 00:04:52,210
+systemic infection. طبعاً هذه systemic infection إيش
+
+67
+00:04:52,210 --> 00:04:55,870
+يعني؟ عدوى بتدخل الجسم وممكن تروح لأي مكان في الجسم
+
+68
+00:04:55,870 --> 00:05:01,420
+وتسبب لكِ أمراض. Wearing personal protective equipment،
+
+69
+00:05:01,420 --> 00:05:06,220
+لازم نلبس، اللي هي الـ gloves، الـ lab coat، الـ eye
+
+70
+00:05:06,220 --> 00:05:11,020
+protection. ممنوع أنا أشتغل في معمل الـ microbiology
+
+71
+00:05:11,020 --> 00:05:15,220
+أزرع بكتيريا بدون ما ألبس الـ coat والـ gloves، ما
+
+72
+00:05:15,220 --> 00:05:18,980
+بضمن أنه أي شيء يدخل جسمي ويسبب لي infection. الـ
+
+73
+00:05:18,980 --> 00:05:22,480
+eye protection، اللي هي الـ goggles، أنا بعض الأحيان
+
+74
+00:05:22,480 --> 00:05:25,880
+ممكن أستخدم الـ eye protection للتغطية، لأن ممكن
+
+75
+00:05:25,880 --> 00:05:29,810
+نزرع بكتيريا سائلة. اتكلمنا عن الأوساط الغذائية في
+
+76
+00:05:29,810 --> 00:05:33,710
+المحاضرة التّعريفية، في بعض أنواع الـ media ممكن أنها
+
+77
+00:05:33,710 --> 00:05:36,870
+تكون، الأوساط الغذائية تكون سائلة. روحت أنتَ زرعتَ
+
+78
+00:05:36,870 --> 00:05:40,670
+بكتيريا، ممكن تيجي على عينك، فبالتالي الـ goggles
+
+79
+00:05:40,670 --> 00:05:44,810
+اللي هي الـ eye protection، هنا مهمة حتى أمنع أنها
+
+80
+00:05:44,810 --> 00:05:48,750
+تيجي على عيني وتسبب لي عدوى. Never applying
+
+81
+00:05:48,750 --> 00:05:53,280
+cosmetics. ممنوع إني أنا، طبعاً، أحط أدوات تجميل، or
+
+82
+00:05:53,280 --> 00:05:56,480
+putting objects, fingers, pencils in the mouth or
+
+83
+00:05:56,480 --> 00:06:01,200
+touching the face. ممنوع تمسك أصابعك أو القلم، طبعاً،
+
+84
+00:06:01,200 --> 00:06:05,960
+سواء في فمك أو أنه يصير touch في الوجه. في كتير
+
+85
+00:06:05,960 --> 00:06:10,640
+بنات مثلاً بتروح تزرع بكتيريا، بعد ما تزرع، بتستنى
+
+86
+00:06:10,640 --> 00:06:14,500
+صاحبتها، قبل ما تخلص، بتضلها في الـ... طبعاً في الـ...
+
+87
+00:06:14,500 --> 00:06:18,950
+في الـ group، بعدها تقعد تستنى، بتروح حاطة اللي هي
+
+88
+00:06:18,950 --> 00:06:22,730
+إيديها على وجهها، بالتالي يصير في عندي touch بين الـ
+
+89
+00:06:22,730 --> 00:06:27,970
+... بين البكتيريا والوجه، ممكن يسبب لها عدوى ومشاكل
+
+90
+00:06:27,970 --> 00:06:32,490
+في الوجه. Never eating, drinking, or chewing gum in
+
+91
+00:06:32,490 --> 00:06:35,530
+the laboratory. ممنوع الأكل والشرب، لأنه ممكن
+
+92
+00:06:35,530 --> 00:06:42,470
+يتلوّث الطعام والشراب بهذه البكتيريا. بالنسبة
+
+93
+00:06:42,470 --> 00:06:47,300
+ لمنطقة العمل، for working: washing hands before and
+
+94
+00:06:47,300 --> 00:06:51,120
+after lab working with water and soap. ضروري بدنا
+
+95
+00:06:51,120 --> 00:06:56,000
+نغسل إيدينا قبل وبعد الشغل بالميّة والصابون. Keep
+
+96
+00:06:56,000 --> 00:07:00,280
+the lab bench free of unnecessary materials. ضروري
+
+97
+00:07:00,280 --> 00:07:04,600
+مكان العمل أو منطقة العمل، فقط الأشياء الضرورية
+
+98
+00:07:04,600 --> 00:07:07,580
+اللي أنا بحتاجها خلال شغلي، إنّه تكون، الأشياء الغير
+
+99
+00:07:07,580 --> 00:07:12,340
+ضرورية، طبعاً ممنوع إنّه نحطّها، حتى لأنّه ممكن إنها
+
+100
+00:07:12,340 --> 00:07:17,160
+تكون مصدر للعدوى فيما بعد. Disinfecting lab bench
+
+101
+00:07:17,160 --> 00:07:21,440
+before and at the conclusion of each lab
+
+102
+00:07:21,440 --> 00:07:26,420
+session. بقول لك هاي disinfecting، هاي معناها إنّا بدنا
+
+103
+00:07:26,420 --> 00:07:32,200
+نطهر الـ bench قبل ما أبدأ شغلي، حتى إني أنا أضمن
+
+104
+00:07:32,200 --> 00:07:35,960
+إنّه ما في أي شيء على الـ bench في هذه المنطقة، أي
+
+105
+00:07:35,960 --> 00:07:41,960
+بكتيريا، أي contamination. وكمان بعد ما أخلص شغلي
+
+106
+00:07:41,960 --> 00:07:47,480
+في المعمل، ضروري إنّه احنا نعقم المكان ونطهّره. يبقى في عندي أنا مصطلحين، disinfection وفي عندي
+
+107
+00:07:47,480 --> 00:07:51,740
+مصطلح ثاني، sterilization. هذول المصطلحين بشكل مختصر،
+
+108
+00:07:51,740 --> 00:07:56,360
+الفرق بينهم، الـ disinfection معناها تطهير، بدي أقتل
+
+109
+00:07:56,360 --> 00:08:01,620
+فقط الـ pathogenic bacteria. الـ disinfection معناها
+
+110
+00:08:01,620 --> 00:08:05,770
+تطهير، كل pathogenic bacteria فقط، بدي أقتل البكتيريا
+
+111
+00:08:05,770 --> 00:08:10,330
+اللي بتسبب لي أمراض. بالنسبة لـ sterilization اللي
+
+112
+00:08:10,330 --> 00:08:14,630
+هي التعقيم، التعقيم، إني أقتل all microorganisms or
+
+113
+00:08:14,630 --> 00:08:20,990
+kill all bacteria، بدي أقتل كل البكتيريا. في عندنا
+
+114
+00:08:20,990 --> 00:08:25,330
+جهاز بنستخدمه لـ sterilization، هنتحدّث فيه في الجزء
+
+115
+00:08:25,330 --> 00:08:30,840
+الأخير من المعمل، هذا الجهاز اسمه الـ Autoclave.
+
+116
+00:08:30,840 --> 00:08:34,500
+بندخل كل الأدوات اللي أنا بدي أشتغل فيها، من loop،
+
+117
+00:08:34,500 --> 00:08:38,880
+من tubes وغيرها من الأمور اللي أنا بستخدمها في شغلي،
+
+118
+00:08:38,880 --> 00:08:45,120
+وبدي أدخلها في جهاز الـ Autoclave. جهاز الـ
+
+119
+00:08:48,480 --> 00:08:52,620
+Autoclave، ضغط عالي ودرجة حرارة عالية، بيقتل كل
+
+120
+00:08:52,620 --> 00:08:56,200
+البكتيريا اللي موجودة في الأدوات، وهكذا بوفّر لي
+
+121
+00:08:56,200 --> 00:09:01,520
+ظروف معقّمة. Good lab practice، بقول لك لو أنا مارست
+
+122
+00:09:01,520 --> 00:09:05,900
+عملي بشكل جيد، ولو اتبعت كل إجراءات الوقاية
+
+123
+00:09:05,900 --> 00:09:10,220
+والسلامة، including methods for aseptic transfer،
+
+124
+00:09:10,220 --> 00:09:16,120
+returning materials to their proper locations, proper care
+
+125
+00:09:16,120 --> 00:09:19,840
+and handling of equipment. نقلت المواد للمكان
+
+126
+00:09:19,840 --> 00:09:24,450
+الصحيح بالطريقة الصحيحة، بالطريقة standard. كل هذه
+
+127
+00:09:24,450 --> 00:09:28,090
+الأمور لو أنا اتبعتها، هحمي حالي من أي infectious
+
+128
+00:09:28,090 --> 00:09:33,350
+من أي عدوى، من أي infection. Keeping the bench top
+
+129
+00:09:33,350 --> 00:09:37,830
+clear of extraneous material. اتكلمنا فيها، إني أنا
+
+130
+00:09:37,830 --> 00:09:42,760
+لازم أحافظ على bench top، يعني سطح الـ bench، لازم
+
+131
+00:09:42,760 --> 00:09:47,280
+أحافظ عليه من أي أمور أنا ما بدي أحتاجها، أي أمور
+
+132
+00:09:47,280 --> 00:09:50,680
+unnecessary، لازم إني أنا أتخلّص منها، فقط الأمور
+
+133
+00:09:50,680 --> 00:09:53,720
+الضرورية اللي بحتاجها في شغلي لازم تكون على الـ
+
+134
+00:09:53,720 --> 00:09:58,660
+bench. هلّأ في كل group هيكون طبعاً، هيكون في عنده
+
+135
+00:09:58,660 --> 00:10:05,880
+لهب بنسن. هذا اللهب، هنوزّعه للبنات جروبات، لما أنا
+
+136
+00:10:05,880 --> 00:10:09,880
+أبدأ أشتغل، أول ما أبدأ أشتغل، لما نخلص الجزء النظري
+
+137
+00:10:09,880 --> 00:10:14,400
+ونبدأ في الجزء العملي، بدنا نغسل أيدينا بالميّة
+
+138
+00:10:14,400 --> 00:10:20,320
+والصابون ونشفّهم منيح. بعد كده باجي لمنطقة الـ bench،
+
+139
+00:10:20,320 --> 00:10:25,240
+كل group بيروح لمنطقة العمل الخاصة فيه وبيجيب
+
+140
+00:10:25,240 --> 00:10:30,000
+detol، بالـ tissue وبيطهر المكان. بعد ما أخلص، بالـ
+
+141
+00:10:30,000 --> 00:10:35,060
+detol، بدي أعقم بالكحول، بجيب tissue وكحول وبعقم
+
+142
+00:10:35,060 --> 00:10:39,610
+الـ bench. بعد كده بدي أبدأ الشغل، بشغّل اللهب، بعد ما
+
+143
+00:10:39,610 --> 00:10:45,450
+أخلص الشغل، بسكّر اللهب، وبعدين بجيب ديطول كحول و
+
+144
+00:10:45,450 --> 00:10:52,930
+برش. ممنوع أنا يكون اللهب شغال وأروح أجي أجي أرش
+
+145
+00:10:52,930 --> 00:10:59,250
+الكحول وأعقم المكان. ليش؟ لأن وقتها هيصير عندي
+
+146
+00:10:59,250 --> 00:11:03,590
+طبعاً انفجار في المكان. ممنوع الكحول واللهب يشتموا.
+
+147
+00:11:04,690 --> 00:11:08,070
+هذا بالنسبة لمنطقة العمل. شو الإجراءات اللي احنا
+
+148
+00:11:08,070 --> 00:11:14,710
+بنعملها قبل وبعد شغلي؟ Dispose
+
+149
+00:11:14,710 --> 00:11:19,250
+all contaminated materials in autoclave bags and
+
+150
+00:11:19,250 --> 00:11:22,530
+prepare for disposal of other different types of waste.
+
+151
+00:11:23,200 --> 00:11:27,660
+الـ dispose يعني بدي أتخلص من all contaminated
+
+152
+00:11:27,660 --> 00:11:31,420
+materials، يعني مواد ملوّثة، بدي أتخلص من
+
+153
+00:11:31,420 --> 00:11:35,460
+كل المواد الملوّثة اللي أنا استخدمتها في شغلي، من Petri dishes، من الـ loops، كل هذه الأدوات بعد ما أخلص، تعتبر
+
+154
+00:11:35,460 --> 00:11:38,880
+contaminated، تعتبر ملوّثات، contaminated فيها بكتيريا،
+
+155
+00:11:38,880 --> 00:11:43,480
+فبالتالي لازم أحطّها في شيء اسمه autoclave bag. الـ
+
+156
+00:11:48,220 --> 00:11:52,970
+autoclave bag هي عبارة عن كيس. بعد ما أخلص الشغل، بحطّ
+
+157
+00:11:52,970 --> 00:11:56,230
+الأطباق اللي زرعتها، وبحطّها في هذا الـ
+
+158
+00:11:56,230 --> 00:11:59,670
+case. ليش بحطّها في هذا الـ case؟ عشان ندخلها في
+
+159
+00:11:59,670 --> 00:12:04,350
+جهاز الـ autoclave عشان نعقّمها، وهيك نحمي حتى عمّال
+
+160
+00:12:04,350 --> 00:12:08,610
+النظافة من الـ infection. The lab coat should be
+
+161
+00:12:08,610 --> 00:12:13,280
+kept separately from other things in your bag. وقول لك
+
+162
+00:12:13,280 --> 00:12:16,420
+الـ lab coat، لازم إحنا نجيب كيس، معمل الـ microbiology،
+
+163
+00:12:16,420 --> 00:12:20,300
+وكيس خاص ونحطه فيه. ممنوع أمسك الـ lab coat وأحطه
+
+164
+00:12:20,300 --> 00:12:24,020
+في الشنطة، لأن خلال شغلك ما بتضمني إنّه يكون بكتيريا
+
+165
+00:12:24,020 --> 00:12:27,400
+اجت على الـ lab coat، روح تحطي الـ lab coat في شنطتك،
+
+166
+00:12:27,400 --> 00:12:32,160
+بالتالي هي كل الأدوات في الشنطة، هتصير ملوّثة
+
+167
+00:12:32,160 --> 00:12:36,220
+بالبكتيريا، وممكن إنها تسبب infection لكِ.
+
+168
+00:12:36,220 --> 00:12:42,700
+Reporting all injuries, accidents, spills. بقول لك لازم
+
+169
+00:12:42,700 --> 00:12:48,600
+إحنا نبلغ عن كل الحوادث، كل الـ spills، إيش يعني الـ spills؟ الـ
+
+170
+00:12:48,600 --> 00:12:54,820
+spills يعني الانسكابات، and broken glassware. الـ
+
+171
+00:12:54,820 --> 00:13:00,120
+أشياء المكسورة. ضروري جداً إنّه طبعاً ينبّه الـ
+
+172
+00:13:00,120 --> 00:13:03,640
+instructor، الـ instructor يعني اللي بيعطي التعليمات،
+
+173
+00:
+
+223
+00:16:31,040 --> 00:16:35,660
+ما اتسببها ايه الأدوات الحادة to prevent needle stick
+
+224
+00:16:35,660 --> 00:16:39,400
+injuries needle shouldn't be recapped ممنوع  أن
+
+225
+00:16:39,400 --> 00:16:43,080
+حتى أني أنا أحملها حالي من غزة الإبرة ممنوع أني أرجع
+
+226
+00:16:43,080 --> 00:16:47,740
+أحط الغطاية  فتحة الـ needle ممنوع أن أرجع
+
+227
+00:16:47,740 --> 00:16:54,640
+أغطيها بغطائها avoid contaminating the
+
+228
+00:16:54,640 --> 00:16:58,180
+outside of the container container طبعاً الـ safety
+
+229
+00:16:58,180 --> 00:17:01,680
+box هذا اللي عارفينه الـ safety box بنعرف لازم أن
+
+230
+00:17:01,680 --> 00:17:05,580
+احنا ناخد بالنا أنه ما يتلوث من برا ببكتيريا على
+
+231
+00:17:05,580 --> 00:17:09,400
+ سبيل المثال روحت انت زراعتي بكتيريا بالـ loop اللوب
+
+232
+00:17:09,400 --> 00:17:12,960
+بظل فيها بكتيريا بالغلط صار عندي touch بالـ
+
+233
+00:17:12,960 --> 00:17:17,600
+container من برا  كيك صار الـ container ملوث ممكن
+
+234
+00:17:17,600 --> 00:17:23,560
+تيجي زميلتك يتفكروا أنه نظيف يصير عندي عدوى ويسبب
+
+235
+00:17:23,560 --> 00:17:28,040
+لها مرض عشان هيك من أول ما اتكلمنا عن الـ Safety
+
+236
+00:17:28,040 --> 00:17:31,280
+Rules قلنا لازم نتعامل مع أي شيء في معمل الـ
+
+237
+00:17:31,280 --> 00:17:35,620
+Microbiology كأنه infectious كأنه معدي All
+
+238
+00:17:35,620 --> 00:17:39,000
+specimens should be bought in a well-constructed
+
+239
+00:17:39,000 --> 00:17:42,280
+container with a secure lid to prevent leaking
+
+240
+00:17:42,280 --> 00:17:46,840
+during transport قلنا كل العينات لازم أني أنا
+
+241
+00:17:46,840 --> 00:17:52,760
+أحطها في وعاء محكم الإغلاق with a secure lid وكمان
+
+242
+00:17:52,760 --> 00:17:58,300
+غطاء آمن ليش؟ حتى أمنع تسرب العينة أثناء عملية
+
+243
+00:17:58,300 --> 00:18:02,200
+النقل ممكن لو كانت العينة سائلة وكان الغطاء مش
+
+244
+00:18:02,200 --> 00:18:05,460
+محكم وأنا روحت نقلت العينة خلال نقل العينة ممكن
+
+245
+00:18:05,460 --> 00:18:11,660
+هي العينة تسير في عندي مثلاً تسريب لمناطق أنا مش
+
+246
+00:18:11,660 --> 00:18:15,820
+ماخدة بالي منها والعينة ممكن يكون فيها طبعاً
+
+247
+00:18:15,820 --> 00:18:22,200
+بكتيريا وتسبب لي عدوى لناس طبعاً خارج حتى المجال
+
+248
+00:18:22,200 --> 00:18:26,060
+الصحي عشان هيك لازم ناخد بالنا أنه العينات كلها
+
+249
+00:18:26,060 --> 00:18:30,840
+نحطها في well-constructed container with a secure
+
+250
+00:18:30,840 --> 00:18:35,780
+lid after they are used disposable syringe and
+
+251
+00:18:35,780 --> 00:18:39,320
+needle scalpel blades and other sharps should be
+
+252
+00:18:39,320 --> 00:18:42,560
+placed in a puncture resistant container for
+
+253
+00:18:42,560 --> 00:18:47,820
+disposable احنا قلنا في الـ .. أول ما تكلمنا عن الـ
+
+254
+00:18:47,820 --> 00:18:51,840
+safety rules الأدوات اللي بعد ما أنا أخلص بحطها في
+
+255
+00:18:51,840 --> 00:18:55,040
+Autoclave Bag الـ Autoclave Bag هو عبارة عن كيس
+
+256
+00:18:55,040 --> 00:18:59,520
+هلق الـ Autoclave Bag من حط فيها الأدوات البلاستيك
+
+257
+00:18:59,520 --> 00:19:02,820
+زي الـ Petri dish زي الـ Loops لكن الأدوات الحادة
+
+258
+00:19:02,820 --> 00:19:07,160
+زي الـ Needles، Scalpels، الـ Sharps هاي كلها
+
+259
+00:19:07,160 --> 00:19:10,520
+بحطها ضروري حتى أني أنا أتخلص منها بحطها في
+
+260
+00:19:10,520 --> 00:19:15,560
+Puncture-resistant container يعني وعاء مقاوم للخرق
+
+261
+00:19:15,560 --> 00:19:20,340
+لأنّي أنا لو حطيتها بالكيس ممكن لو حطيت الـ needle في
+
+262
+00:19:20,340 --> 00:19:24,620
+كيس اللي هو الـ autoclave bag ممكن تخرق الكيس وهيك
+
+263
+00:19:24,620 --> 00:19:28,180
+بالتالي ممكن أنه تسبب إصابة لإنسان اللي بيحملها
+
+264
+00:19:28,180 --> 00:19:32,120
+عشان هيك الأدوات الحادة أنا بتخلص منها بحطها في
+
+265
+00:19:32,120 --> 00:19:35,160
+puncture resistant container لكن الأدوات مثلاً
+
+266
+00:19:35,160 --> 00:19:37,720
+البلاستيك بحطها في الـ autoclave bag
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/wyOj5_y9CBQ_postprocess.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/wyOj5_y9CBQ_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..706ffcd38a80c1c48a7d9731aa725acadb3017a8
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/wyOj5_y9CBQ_postprocess.srt
@@ -0,0 +1,1064 @@
+1
+00:00:00,880 --> 00:00:04,040
+بدنا نحكي في هذه الـ slide عن الـ safety rules عن
+
+2
+00:00:04,040 --> 00:00:08,300
+قواعد وإجراءات السلامة والوقاية داخل معمل الـ
+
+3
+00:00:08,300 --> 00:00:11,440
+microbiology in the microbiology laboratory
+
+4
+00:00:11,440 --> 00:00:14,480
+infectious materials are processed as the money of
+
+5
+00:00:14,480 --> 00:00:17,480
+the microorganism used in this course may be
+
+6
+00:00:17,480 --> 00:00:20,960
+pathogenic for a human في معمل ال microbiology
+
+7
+00:00:20,960 --> 00:00:25,480
+بدنا نتعامل مع أي إشي كأنه infectious كأنه معدي
+
+8
+00:00:25,480 --> 00:00:29,300
+وممكن يسببلي مرض كأنه pathogenic for a human ممكن
+
+9
+00:00:29,300 --> 00:00:33,600
+أنهلسبب مرض لأ الإنسان احنا هنتعامل مع بكتيريا
+
+10
+00:00:33,600 --> 00:00:37,660
+هنصبخ بكتيريا هنجري فحوصات على بكتيريا هتزرعي
+
+11
+00:00:37,660 --> 00:00:42,420
+بكتيريا هتمسكي طبق في مزروع عليه بكتيريا عشان هيك
+
+12
+00:00:42,420 --> 00:00:46,900
+أي طبق احنا بنعطيكيه اعتبريه انه infectious انه
+
+13
+00:00:46,900 --> 00:00:51,360
+معدي وممكن يسبب اليك مرض also the materials found
+
+14
+00:00:51,940 --> 00:00:55,740
+برضه الأدوات الموجودة في المعمل such as glass
+
+15
+00:00:55,740 --> 00:00:59,340
+equipment زي زجاجيات من baker, flask, cylinder,
+
+16
+00:00:59,500 --> 00:01:03,460
+tube, biological agent and chemical المواد
+
+17
+00:01:03,460 --> 00:01:06,500
+الكيميائية برضه بدي أتعامل معها كإنها infectious
+
+18
+00:01:06,500 --> 00:01:10,920
+كإنها معضية وممكن إنها تسببلي مرض Campos safety
+
+19
+00:01:10,920 --> 00:01:15,640
+hazard ممكن إنها تشكل خطر على حياتناIf you don't
+
+20
+00:01:15,640 --> 00:01:18,960
+follow laboratory protocol إذا ما اتبعنا قواعد
+
+21
+00:01:18,960 --> 00:01:24,940
+السلامة وامان داخل معمل الـ microbiology As a
+
+22
+00:01:24,940 --> 00:01:28,920
+result لذا قالك certain rules هناك قواعد are
+
+23
+00:01:28,920 --> 00:01:31,680
+necessary to prevent the spread of infectious
+
+24
+00:01:31,680 --> 00:01:37,340
+agent ضروري أن نلتزم فيها حتى نمنع انتشار العدوى
+
+25
+00:01:37,340 --> 00:01:41,500
+The possibility of infection yourselves or other
+
+26
+00:01:41,500 --> 00:01:46,540
+peopleوحتى أقلل فرصة احتمالية اصابة نفسي او حد
+
+27
+00:01:46,540 --> 00:01:50,440
+قريب مني بالعدوى كيف ممكن حد قريب مني بالعدوى
+
+28
+00:01:50,440 --> 00:01:54,860
+ينصاب روحتي انت على سبيل المثال زراعتي بكتيريا بعد
+
+29
+00:01:54,860 --> 00:01:59,460
+ما زراعتي حطيت ايدك على ال bench طبعا هيك ال bench
+
+30
+00:01:59,460 --> 00:02:03,920
+بيصير ملوث بالبكتيريا اجت صحابيتك حطت قلم على ال
+
+31
+00:02:03,920 --> 00:02:08,060
+benchخلص المعمل أخدت القلم و حطته بالشنطة بعد ما
+
+32
+00:02:08,060 --> 00:02:11,840
+روحت على البيت اجى أخوها الصغير و حط هذا القلم في
+
+33
+00:02:11,840 --> 00:02:17,120
+تمه هان الأخوها الصغير اتعرض لعدوى نتيجة عدم
+
+34
+00:02:17,120 --> 00:02:20,540
+اتباعها إجراءات السلامة و البقايا في معمل الـ
+
+35
+00:02:20,540 --> 00:02:24,900
+Microbiology عشان هيك لازم نتبع هذه الإجراءات حتى
+
+36
+00:02:24,900 --> 00:02:31,630
+نحمي نفسنا و نحمي كل قريبنا من العدوىبرضه الهاتف
+
+37
+00:02:31,630 --> 00:02:35,250
+ممنوع نمسكه في معمل الـ Microbiology أي أدوات خلال
+
+38
+00:02:35,250 --> 00:02:39,430
+الشغل أنا بدي استخدمها ضروري بعد ما أنا قبل ما
+
+39
+00:02:39,430 --> 00:02:46,810
+أطلع من معمل الـ Microbiology إن أنا انعقبهم All
+
+40
+00:02:46,810 --> 00:02:50,070
+healthcare workers should routinely use
+
+41
+00:02:50,070 --> 00:02:54,410
+appropriate barrier evacuation قلنا كل العاملين في
+
+42
+00:02:54,410 --> 00:03:01,130
+المجال الصحي لازم يستخدموا بشكل روتينيprecuation
+
+43
+00:03:01,130 --> 00:03:05,170
+يعني وسائل حماية مناسبة لازم يستخدموا وسائل حماية
+
+44
+00:03:05,170 --> 00:03:10,770
+مناسبة to prevent skin حتى يحموا جلدهم add mucus
+
+45
+00:03:10,770 --> 00:03:16,130
+membrane الأخشية المخاطية exposure من أنه تتعرض
+
+46
+00:03:16,130 --> 00:03:20,490
+when contact with blood or other body fluid of any
+
+47
+00:03:20,490 --> 00:03:25,230
+patient حتى أنه تتعرض لهذا ال pathogen أو
+
+48
+00:03:25,230 --> 00:03:29,490
+البكتيريا من العينات المختلفة سواء من blood أو من
+
+49
+00:03:29,720 --> 00:03:33,800
+سوائل الجسم المختلفة طيب كيف العاملين في المجال
+
+50
+00:03:33,800 --> 00:03:37,880
+الصحي بيحموا جلدهم بيلبسوا من وسائل الحماية أنهم
+
+51
+00:03:37,880 --> 00:03:41,700
+يلبسوا ل gloves and mucus membrane كيف بدي أحمي
+
+52
+00:03:41,700 --> 00:03:46,240
+الأغشية المخاطية أني أنا ألبس ال mask تمام بالنسبة
+
+53
+00:03:46,240 --> 00:03:50,100
+لقواعة السلامة أهم إشي أني أنا أبدأ بحماية نفسي
+
+54
+00:03:50,100 --> 00:03:55,600
+لأنه لو حاميت نفسي أكيد هحمي الآخرين من العدوى أول
+
+55
+00:03:55,600 --> 00:04:00,780
+نقطة هي للناس اللي برا لأنهاحنا محجبين للناس اللي
+
+56
+00:04:00,780 --> 00:04:05,400
+بشعرهم بقولك لازم انك انت اتحافظي على شعرك يكون
+
+57
+00:04:05,400 --> 00:04:10,880
+مربوط لأنه ممكن خلال شغلك اذا ال كان مش مربوط
+
+58
+00:04:10,880 --> 00:04:15,500
+الشعر ينزل على الطبق البكتيري always wearing
+
+59
+00:04:15,500 --> 00:04:20,980
+closed shoes في معمل ال microbiology لازم ال shoes
+
+60
+00:04:20,980 --> 00:04:25,320
+يكون closed ممنوع نلبس صنادر لأنه ممكن يكون في
+
+61
+00:04:25,320 --> 00:04:32,090
+رجلك يكون في جلحروحتي انت زراعتي بكتيريا طبعا خلال
+
+62
+00:04:32,090 --> 00:04:35,790
+الزراعة مثلا انا بستخدم فيه أداء اسمه الوب في
+
+63
+00:04:35,790 --> 00:04:40,990
+الزراعة وقعت هذه اللوب على الأرض وإجت على رجلك
+
+64
+00:04:40,990 --> 00:04:44,210
+اللي فيها الجروح طبعا اللوب فيها بكتيريا هذه
+
+65
+00:04:44,210 --> 00:04:48,410
+البكتيريا هتدخل من هذا الجروح للجسم وتعملك
+
+66
+00:04:48,410 --> 00:04:52,210
+systemic infection طبعا هذه systemic infection إيش
+
+67
+00:04:52,210 --> 00:04:55,870
+يعني عدوى بتدخل قدامه وممكن تروح لأى مكان في الجسم
+
+68
+00:04:55,870 --> 00:05:01,420
+وتسببلك أمراضwearing personal protection equipment
+
+69
+00:05:01,420 --> 00:05:06,220
+لازم نلبس اللي هي ال gloves ال lab coat ال eye
+
+70
+00:05:06,220 --> 00:05:11,020
+protection ممنوع أنا أشتغل في معمل ال microbiology
+
+71
+00:05:11,020 --> 00:05:15,220
+أزرع بكتيريا بدون ما ألبس ال coat و ال gloves ما
+
+72
+00:05:15,220 --> 00:05:18,980
+بضمن أنه أي إشي يدخل جسمي و يسببلي infection ال
+
+73
+00:05:18,980 --> 00:05:22,480
+eye protection اللي هي ال googles أنا بعض الأحيان
+
+74
+00:05:22,480 --> 00:05:25,880
+ممكن أستخدم ال eye protection لالتغطية لأن ممكن
+
+75
+00:05:25,880 --> 00:05:29,810
+نزرع بكتيريا سائلةأتكلمنا عن الأوساط الغذائية في
+
+76
+00:05:29,810 --> 00:05:33,710
+المحاضرة التعليفية في بعض أنواع ال media ممكن أنها
+
+77
+00:05:33,710 --> 00:05:36,870
+تكون الأوساط الغذائية تكون سائلة روحت أنت زرعت
+
+78
+00:05:36,870 --> 00:05:40,670
+بكتيريا ممكن تيجي على عينك فبالتالي ال googles
+
+79
+00:05:40,670 --> 00:05:44,810
+اللي هي ال eye protection هان مهمة حتى أمنع أنها
+
+80
+00:05:44,810 --> 00:05:48,750
+تيجي على عيني و تسببلي عدوى never applying
+
+81
+00:05:48,750 --> 00:05:53,280
+cosmetics ممنوع أني أناطبعا أحط أدوات تجميل or
+
+82
+00:05:53,280 --> 00:05:56,480
+blessing object finger pencil in the mouth or
+
+83
+00:05:56,480 --> 00:06:01,200
+touching the face ممنوع تمسك أصابعك أو القلم طبعا
+
+84
+00:06:01,200 --> 00:06:05,960
+سواء في طمك أو أنه يصير touch في الوجه في كتير
+
+85
+00:06:05,960 --> 00:06:10,640
+بنات مثلا بتروح تزرع بكتيريا بعد ما تزرع بتهتستنى
+
+86
+00:06:10,640 --> 00:06:14,500
+صحبيتها قبل ما تخلص بتضلها في ال .. طبعا في ال ..
+
+87
+00:06:14,500 --> 00:06:18,950
+في ال groupبعدها تقعد تستنى بتروح حاطة اللى هى
+
+88
+00:06:18,950 --> 00:06:22,730
+إيديها على وجهها بالتالي يصير في عندي touch بين ال
+
+89
+00:06:22,730 --> 00:06:27,970
+.. بين البكتيريا و الوجه ممكن يسببلها عدوى و مشاكل
+
+90
+00:06:27,970 --> 00:06:32,490
+في الوجهة never eating, drinking or chewing gum in
+
+91
+00:06:32,490 --> 00:06:35,530
+the laboratory ممنوع الأكل و الشراب لأنه ممكن
+
+92
+00:06:35,530 --> 00:06:42,470
+يتلوث الطعام و الشراب بهذه البكتيريا بالنسبة
+
+93
+00:06:42,470 --> 00:06:47,300
+لمنطقة الشغالfor working washing hand period and
+
+94
+00:06:47,300 --> 00:06:51,120
+after lab working with water and soap ضروري بدنا
+
+95
+00:06:51,120 --> 00:06:56,000
+نغسل إيدينا قبل و بعد الشغل بالمية والصابون keep
+
+96
+00:06:56,000 --> 00:07:00,280
+the lab bench free of unnecessary material ضروري
+
+97
+00:07:00,280 --> 00:07:04,600
+المكان الشغل أو منطقة الشغل فقط الأشياء الضرورية
+
+98
+00:07:04,600 --> 00:07:07,580
+اللي أنا بحتاجها خلال شغلي انها تكون الأشياء الغير
+
+99
+00:07:07,580 --> 00:07:12,340
+ضروريةطبعا ممنوع أنها نحطها حتى لأنه ممكن أنها
+
+100
+00:07:12,340 --> 00:07:17,160
+تكون مصدر للعدوى فيما بعد disinfecting lab bench
+
+101
+00:07:17,160 --> 00:07:21,440
+period to end at the conclusion of each lab
+
+102
+00:07:21,440 --> 00:07:26,420
+session بقولك ها disinfecting هاي معناها أننا بدنا
+
+103
+00:07:26,420 --> 00:07:32,200
+نطهر ال bench ما قبل ما أبدأ شغلي حتى أني أنا أغمن
+
+104
+00:07:32,200 --> 00:07:35,960
+أنه مافي أي إشي على ال bench في هذه المنطقة أي
+
+105
+00:07:35,960 --> 00:07:41,960
+بكتيريا أي contaminationوكمان بعد ما انت من شغلي
+
+106
+00:07:41,960 --> 00:07:47,480
+في المعمل ضروري انه ان احنا نعقم المكان وينطهره
+
+107
+00:07:47,480 --> 00:07:51,740
+يبقى في عندي انا مصطلحين disinfection وفي عندي
+
+108
+00:07:51,740 --> 00:07:56,360
+مصطلح تاني sterilization هدول المصطلحين بشكل مختصر
+
+109
+00:07:56,360 --> 00:08:01,620
+الفرق بينهم ال disinfection معناه تطهير بدي اقتل
+
+110
+00:08:01,620 --> 00:08:05,770
+فقط ال pathogenic bacteriaالـ disinfection معناها
+
+111
+00:08:05,770 --> 00:08:10,330
+تطهير كل pathogenic بكتيريا فقط بدي أقتل البكتيريا
+
+112
+00:08:10,330 --> 00:08:14,630
+اللي بتسببلي أمراض بالنسبة لـ sterilization اللي
+
+113
+00:08:14,630 --> 00:08:20,990
+هي التعقيم التعقيم انه kill all microorganism or
+
+114
+00:08:20,990 --> 00:08:25,330
+kill all بكتيريا بدي أقتل كل البكتيريا في ان احنا
+
+115
+00:08:25,330 --> 00:08:30,840
+جهاز بنستخدمه لـ sterilization هنتحدث فيهفي الجزء
+
+116
+00:08:30,840 --> 00:08:34,500
+الأخير من المعمل هذا الجهاز اسمه الـ Autoclave
+
+117
+00:08:34,500 --> 00:08:38,880
+مندخل كل الأدوات اللي أنا بدي أشتغل فيها من Loop
+
+118
+00:08:38,880 --> 00:08:45,120
+من Tube وغيرها من الأمور اللي أنا بستخدمها في شغلي
+
+119
+00:08:45,120 --> 00:08:48,480
+وبدي أدخلها في جهاز الـ Autoclave جهاز الـ
+
+120
+00:08:48,480 --> 00:08:52,620
+Autoclave ضغط عالي ودرجة حرارة عالية بيقتل كل
+
+121
+00:08:52,620 --> 00:08:56,200
+البكتيريا اللي موجودة في الأدوات وهكذا بوفر لي
+
+122
+00:08:56,200 --> 00:09:01,520
+ظروف معقمةGood lab practice بقول لها لو أنا مارست
+
+123
+00:09:01,520 --> 00:09:05,900
+عملي بشكل جيد و لو اتبعت كل إجراءات الوقاية
+
+124
+00:09:05,900 --> 00:09:10,220
+والسلامة including method for aseptic transfer
+
+125
+00:09:10,220 --> 00:09:16,120
+returning materials to proper location proper care
+
+126
+00:09:16,120 --> 00:09:19,840
+and handling of equipment نقلت المواد للمكان
+
+127
+00:09:19,840 --> 00:09:24,450
+الصحيح بالطريقة الصحيحة بالطريقة ال standardكل هذه
+
+128
+00:09:24,450 --> 00:09:28,090
+الأمور لو أنا اتبعتها هحمي حالي من اي infectious
+
+129
+00:09:28,090 --> 00:09:33,350
+من اي عدو من اي infection keeping the bench top
+
+130
+00:09:33,350 --> 00:09:37,830
+clear of extraneous material التكلمنا فيها ان انا
+
+131
+00:09:37,830 --> 00:09:42,760
+لازم احافظ على bench top يعني سطح ال benchلازم
+
+132
+00:09:42,760 --> 00:09:47,280
+أحافظ عليه من أي أمور أنا ما بدي أحتاجها أي أمور
+
+133
+00:09:47,280 --> 00:09:50,680
+unnecessary لازم أني أنا أتخلص منها فقط الأمور
+
+134
+00:09:50,680 --> 00:09:53,720
+الضرورية اللي بحتاجها في شغلي لازم تكون على ال
+
+135
+00:09:53,720 --> 00:09:58,660
+«بنش» هلأ في كل group هيكون طبعاً هيكون في عنده
+
+136
+00:09:58,660 --> 00:10:05,880
+لهب «بنسن» هذا اللهب هنوزع البنات جروبات لما أنا
+
+137
+00:10:05,880 --> 00:10:09,880
+أبدأ أشتغل أول ما أبدأ أشتغل لما نخلص الجزء النظر
+
+138
+00:10:09,880 --> 00:10:14,400
+ونبدأ في الجزء العمليبدنا نغسل أيدانا فيه الميه
+
+139
+00:10:14,400 --> 00:10:20,320
+والصابون و نشفهم منيح بعد كده باجي لمنطقة ال bench
+
+140
+00:10:20,320 --> 00:10:25,240
+كل group بيروح لمنطقة الشغل الخاصة فيه و بيجيب
+
+141
+00:10:25,240 --> 00:10:30,000
+detail بال tissue و بيطهر المكان بعد ما أخلص بال
+
+142
+00:10:30,000 --> 00:10:35,060
+detail بدي أعقم بالكحول بجيب tissue و كحول و بعقم
+
+143
+00:10:35,060 --> 00:10:39,610
+ال benchبعد كده بدي أبدأ الشغل بشغل اللهب بعد ما
+
+144
+00:10:39,610 --> 00:10:45,450
+أخلص الشغل بسكر اللهب و بعدين بجيب دي طول كحول و
+
+145
+00:10:45,450 --> 00:10:52,930
+برش ممنوع أنا يكون اللهب شغال و أروح أجي أجي أرش
+
+146
+00:10:52,930 --> 00:10:59,250
+الكحول و أعقم المكان ليش؟ لأن وقتها هيصير عندى
+
+147
+00:10:59,250 --> 00:11:03,590
+طبعا انفجار في المكان ممنوع الكحول و اللهب يشتموا
+
+148
+00:11:04,690 --> 00:11:08,070
+هذا بالنسبة لمنطقة الشغل شو الإجراءات اللي احنا
+
+149
+00:11:08,070 --> 00:11:14,710
+بنعملها ما قبل و بعد شغلي dispose
+
+150
+00:11:14,710 --> 00:11:19,250
+all contaminated material in autoclave bag and
+
+151
+00:11:19,250 --> 00:11:22,530
+prepare disposable of other different type of pest
+
+152
+00:11:23,200 --> 00:11:27,660
+الـ despose يعني بدي أتخلص من all كونتا منادت
+
+153
+00:11:27,660 --> 00:11:31,420
+كونتا منادت material يعني مواد ملوثة بدي أتخلص من
+
+154
+00:11:31,420 --> 00:11:35,460
+كل المواد الملوثة اللي أنا استخدمتها في شغل من بيت
+
+155
+00:11:35,460 --> 00:11:38,880
+الرفاش من اللوب كل هذه الأدوات بعد ما أخلص تعتبر
+
+156
+00:11:38,880 --> 00:11:43,480
+كونتا مناشن تعتبر ملوثات كونتا منادت فيها بكتيريا
+
+157
+00:11:43,480 --> 00:11:48,220
+فبالتالي لازم أحطها في إشي اسمه autoclave bag ال
+
+158
+00:11:48,220 --> 00:11:52,970
+autoclave bag هي عبارة عن كيسبعد ما أخلص الشغل بحط
+
+159
+00:11:52,970 --> 00:11:56,230
+الأطباق اللي بتريدش اللي زراعتها و بحطها في هذا ال
+
+160
+00:11:56,230 --> 00:11:59,670
+case ليش بحطها في هذا ال case؟ عشان يدخلوها في
+
+161
+00:11:59,670 --> 00:12:04,350
+جهاز ال autoclave عشان نعقمها و هيك نحمي حتى عمال
+
+162
+00:12:04,350 --> 00:12:08,610
+مثلاين نظافة من ال infection the code should be
+
+163
+00:12:08,610 --> 00:12:13,280
+kept separately from other things in your bagوقولك
+
+164
+00:12:13,280 --> 00:12:16,420
+الـ code لازم احنا نجيب كيس معمل الـ microbiology
+
+165
+00:12:16,420 --> 00:12:20,300
+وكيس خاص ونحطه فيه ممنوع امسك ال lab code و احطه
+
+166
+00:12:20,300 --> 00:12:24,020
+في الشنطة لأن خلال شغلك ما بتضمني ان يكون بكتيريا
+
+167
+00:12:24,020 --> 00:12:27,400
+اجت على ال lab code روح تحطيها ال lab code في شنطك
+
+168
+00:12:27,400 --> 00:12:32,160
+بالتالي هي كل الأدوات في الشنطة هتصير ملوثة
+
+169
+00:12:32,160 --> 00:12:36,220
+بالبكتيريا وممكن انها تسبب infection لإلك
+
+170
+00:12:36,220 --> 00:12:42,700
+Departing all injury accidents spells بقولنالازم
+
+171
+00:12:42,700 --> 00:12:48,600
+احنا كل الحوادث كل ال spells اش يعني ال spells؟ ال
+
+172
+00:12:48,600 --> 00:12:54,820
+spells يعني الانسكابات and broken ال class where
+
+173
+00:12:54,820 --> 00:13:00,120
+الأشياء المكسورة ضروري جدا انه طبعا ينبه ال
+
+174
+00:13:00,120 --> 00:13:03,640
+instructor ال instructor يعني اللي بيعطي التعليمات
+
+175
+00:13:03,640 --> 00:13:08,780
+and receiving instruction for clean up ونستقبل منه
+
+176
+00:13:08,780 --> 00:13:15,590
+التعليمات كيف انه احنا ممكنان انا بحاول ان اتخلص
+
+177
+00:13:15,590 --> 00:13:19,890
+من هذا ال spells or broken glassware طبعا هنتعرف
+
+178
+00:13:19,890 --> 00:13:23,290
+كمان شوية في كمان slide تان على كيف انا ممكن لو
+
+179
+00:13:23,290 --> 00:13:26,710
+صار في عندي spells لو صار في عندي انسكاب او اشي
+
+180
+00:13:26,710 --> 00:13:31,300
+انكسر ججاجي كيف ان انا اتعامل معاهalways using
+
+181
+00:13:31,300 --> 00:13:34,940
+appropriate bipeding device and understand that
+
+182
+00:13:34,940 --> 00:13:38,440
+mouth bipeding is forbidden عارفين أنتم ال mouth
+
+183
+00:13:38,440 --> 00:13:42,940
+bipeding أكيد مرهت معاكم هاي ممنوع forbidden ممنوع
+
+184
+00:13:42,940 --> 00:13:47,220
+أني أنا أستعملها في معمل ال microbiology لأنه ممكن
+
+185
+00:13:47,220 --> 00:13:50,860
+يكون في عندي بكتيريا فبالتالي أنا أروح أشفط
+
+186
+00:13:50,860 --> 00:13:55,130
+بكتيريا و هيك تسببلي عدوىUsing universal pre
+
+187
+00:13:55,130 --> 00:13:58,670
+-equation post in the lab and seen inside front
+
+188
+00:13:58,670 --> 00:14:03,290
+cover of this laboratory manual Universal يعني
+
+189
+00:14:03,290 --> 00:14:07,370
+عالمي، pre-equation قلنا وسائل حماية في عندي أنا
+
+190
+00:14:07,370 --> 00:14:12,910
+في كل قسم مثلا أو في كل معمل في عندي lab manual أو
+
+191
+00:14:13,860 --> 00:14:19,180
+الكتاب خاص هذا الكتاب بيكون فيه كل فحص كل الفحصات
+
+192
+00:14:19,180 --> 00:14:22,160
+اللي بنعملها في القسم الـ Microbiology اسم هذا
+
+193
+00:14:22,160 --> 00:14:25,520
+الفحص، أشهر الأدوات اللي أنا بستخدمها، شو إجراءات
+
+194
+00:14:25,520 --> 00:14:30,440
+السلامة و الوقاية، و اللي كان لازم انت طبعا تشوفي
+
+195
+00:14:30,440 --> 00:14:33,280
+لو انت دخلت القسم ال Microbiology انك انت تشوفي
+
+196
+00:14:33,280 --> 00:14:40,580
+هذا ال lab manual Safety
+
+197
+00:14:40,580 --> 00:14:45,390
+for dealing with specimenقواعد السلامة والأمان في
+
+198
+00:14:45,390 --> 00:14:49,790
+تعاملنا مع العينة blood and other body fluid from
+
+199
+00:14:49,790 --> 00:14:53,130
+all patients should be considered infective أحنا
+
+200
+00:14:53,130 --> 00:14:57,030
+قلنا أي إشي في معمل الـ microbiology لازم نعتبر أن
+
+201
+00:14:57,030 --> 00:15:00,450
+هو infectious فمبالك في العينات اللي بتيجي من
+
+202
+00:15:00,450 --> 00:15:04,130
+المرضى عشان أنا بدي أفحصها برضه لازم أني أنا
+
+203
+00:15:04,130 --> 00:15:09,510
+أعتبرها أنها معدية ممكن تنقلي العدوىAll healthcare
+
+204
+00:15:09,510 --> 00:15:13,650
+workers should take precaution to prevent injury
+
+205
+00:15:13,650 --> 00:15:18,490
+caused by needle واللي كان كل العاملين في المجال
+
+206
+00:15:18,490 --> 00:15:23,350
+الصحي لازم ياخدوا بالهم ويحموا أنفسهم من ال injury
+
+207
+00:15:23,350 --> 00:15:28,140
+اللي بتسببها من الإصابات الممكن يسببها by needleor
+
+208
+00:15:28,140 --> 00:15:32,000
+scalpels ايش يعني scalpels يعني مش روت and other
+
+209
+00:15:32,000 --> 00:15:35,520
+sharp instrument او الأدوات الحادة or devised
+
+210
+00:15:35,520 --> 00:15:39,780
+during a procedure خلال شغلي انا لازم اخد بالي لو
+
+211
+00:15:39,780 --> 00:15:43,880
+انا استخدمت needle او أدوات حادة حتى اني انا ما
+
+212
+00:15:43,880 --> 00:15:50,900
+طبعا ممكن ال needle انها يسببطبعا تنغز في ال
+
+213
+00:15:50,900 --> 00:15:54,880
+needle و تسببلي عدوى بعض الأحيان ال needle أنا
+
+214
+00:15:54,880 --> 00:15:58,680
+بستخدمها في فحص المضادات الحيوية بروح بزرع بكتيريا
+
+215
+00:15:58,680 --> 00:16:02,000
+في طباق و بعدين بجبدي أحط أقراس ال antibiotic على
+
+216
+00:16:02,000 --> 00:16:06,100
+هذه البكتيريا فبستخدم ال needle في وضع ال
+
+217
+00:16:06,100 --> 00:16:10,200
+antibiotic على البكتيريا فهك ممكن يصير touch بين
+
+218
+00:16:10,200 --> 00:16:14,390
+ال needle و بين البكتيرياوممكن أنا نتيجة طبعا
+
+219
+00:16:14,390 --> 00:16:18,530
+استخدامي اني انا ما اتخدت اجراءاتي الوقاية ممكن
+
+220
+00:16:18,530 --> 00:16:22,470
+انا انغز بالإبرة وهيك طبعا الإبرة فيها بكتيريا و
+
+221
+00:16:22,470 --> 00:16:26,970
+تدخل الرسم وتسببلي infection هان بقولي لازم اني
+
+222
+00:16:26,970 --> 00:16:31,040
+انا ااخد كل الإجراءات حتى امنعالحوادث اللي ممكن
+
+223
+00:16:31,040 --> 00:16:35,660
+اتسببها ايه الأدوات الحادة to prevent needle stick
+
+224
+00:16:35,660 --> 00:16:39,400
+injuries needle shouldn't be recapped ممنوع انا
+
+225
+00:16:39,400 --> 00:16:43,080
+حتى اني انا احمل حالي من غزة الإبرة ممنوع اني ارجع
+
+226
+00:16:43,080 --> 00:16:47,740
+احط الغطاية فتحت ال needle ممنوع اقلط اني ارجع
+
+227
+00:16:47,740 --> 00:16:54,640
+اغطيها بيه اللي هي غطيتهاavoid contaminating the
+
+228
+00:16:54,640 --> 00:16:58,180
+outside of the container container طبعا ال safety
+
+229
+00:16:58,180 --> 00:17:01,680
+box هذا اللي عارفينه ال safety box بنعرف لازم ان
+
+230
+00:17:01,680 --> 00:17:05,580
+احنا ناخد بالنا انه ما يتلوث من برا ببكتيريا على
+
+231
+00:17:05,580 --> 00:17:09,400
+سبيل المثال روحت انت زراعتي بكتيريا بال loop اللوب
+
+232
+00:17:09,400 --> 00:17:12,960
+بظل فيها بكتيريا بالغلط صار عندي touch بال
+
+233
+00:17:12,960 --> 00:17:17,600
+container من برا كيك صار ال container ملوث ممكن
+
+234
+00:17:17,600 --> 00:17:23,560
+تيجي أزميلتك يتفكروا انه نظيف يصير عندي عدوىويسبب
+
+235
+00:17:23,560 --> 00:17:28,040
+لها مرض عشان هيك من أول ما اتكلمنا عن الـ Safety
+
+236
+00:17:28,040 --> 00:17:31,280
+Rules قلنا لازم نتعامل مع أي إشي في معمل الـ
+
+237
+00:17:31,280 --> 00:17:35,620
+Microbiology كإنه infectious كإنه معدي All
+
+238
+00:17:35,620 --> 00:17:39,000
+specimens should be bought in a well-constructed
+
+239
+00:17:39,000 --> 00:17:42,280
+container with a secure lid to prevent leaking
+
+240
+00:17:42,280 --> 00:17:46,840
+during transport قلنا كل العينات لازم أني أنا
+
+241
+00:17:46,840 --> 00:17:52,760
+أحطها في وعاء محكم الإغلاق with a secure lidوكمان
+
+242
+00:17:52,760 --> 00:17:58,300
+غطاء آمن ليش؟ حتى أمنع تسرب العينة أثناء عملية
+
+243
+00:17:58,300 --> 00:18:02,200
+النقل ممكن لو كانت العينة سائلة وكان الغطاء مش
+
+244
+00:18:02,200 --> 00:18:05,460
+محكم و أنا روحت نقلت العينة خلال نقل العينة ممكن
+
+245
+00:18:05,460 --> 00:18:11,660
+هي العينة تسير في عندي مثلا تسريب لمناطق أنا مش
+
+246
+00:18:11,660 --> 00:18:15,820
+ماخدة بالي منها و العينة ممكن يكون فيها طبعا
+
+247
+00:18:15,820 --> 00:18:22,200
+بكتيريا و تسببلي عدوى لناس طبعاخارج حتى المجال
+
+248
+00:18:22,200 --> 00:18:26,060
+الصحي عشان هيك لازم ناخد بالنا إنه العينات كلها
+
+249
+00:18:26,060 --> 00:18:30,840
+نحطها في well-constructed container with a secure
+
+250
+00:18:30,840 --> 00:18:35,780
+lip after they are used disposable syringe and
+
+251
+00:18:35,780 --> 00:18:39,320
+needle scalpel blades and other sharp should be
+
+252
+00:18:39,320 --> 00:18:42,560
+placed in a puncture resistant container for
+
+253
+00:18:42,560 --> 00:18:47,820
+disposable احنا قلنا في ال .. أول ما تكلمنا عن ال
+
+254
+00:18:47,820 --> 00:18:51,840
+safety rulesالأدوات اللي بعد ما انا أخلص بحطها في
+
+255
+00:18:51,840 --> 00:18:55,040
+Auto-Clap Bag الـ Auto-Clap Bag هو عبارة عن كيس
+
+256
+00:18:55,040 --> 00:18:59,520
+هلأ الـ Auto-Clap Bag منحط فيها الأدوات البلاستيك
+
+257
+00:18:59,520 --> 00:19:02,820
+زي الـ Petri dish زي الـ Loops لكن الأدوات الحادة
+
+258
+00:19:02,820 --> 00:19:07,160
+زي الـ Needles، Scalpels، الـ Sharps هاي كلها
+
+259
+00:19:07,160 --> 00:19:10,520
+بحطها ضروري حتى أني انا أتخلص منها بحطها في
+
+260
+00:19:10,520 --> 00:19:15,560
+Puncture-resistant container يعني وعاء مقاوم للخرق
+
+261
+00:19:15,560 --> 00:19:20,340
+لأني انا لو حطيتها للكيسممكن لو حطيت الـ needle في
+
+262
+00:19:20,340 --> 00:19:24,620
+كيس اللي هو الـ autoclave bag ممكن تخرق الكيس وهيك
+
+263
+00:19:24,620 --> 00:19:28,180
+بالتالي ممكن أنه تسبب أداء لإنسان اللي بيحملها
+
+264
+00:19:28,180 --> 00:19:32,120
+عشان هيك الأدوات الحادة أنا بتخلص منها بحطها في
+
+265
+00:19:32,120 --> 00:19:35,160
+puncture resistant container لكن الأدوات مثلا
+
+266
+00:19:35,160 --> 00:19:37,720
+لبلاستيك بحطها في ال autoclave bag
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/yt25unVl2bQ.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/yt25unVl2bQ.srt
new file mode 100644
index 0000000000000000000000000000000000000000..181b2a37d87732fedf72e023eb5b46dac6aac7ab
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/yt25unVl2bQ.srt
@@ -0,0 +1,1602 @@
+1
+00:00:01,960 --> 00:00:05,660
+السلام عليكم ورحمة الله وبركاته. نستكمل معكم
+
+2
+00:00:05,660 --> 00:00:09,700
+محاضرات مساق  Microbiology العملي، ولازلنا في
+
+3
+00:00:09,700 --> 00:00:13,200
+الـ biochemical test. اليوم موضوعنا سيكون عن
+
+4
+00:00:13,200 --> 00:00:17,240
+bacterial fermentation and methyl red Voges-Proskauer.
+
+5
+00:00:17,240 --> 00:00:21,440
+هنبدأ بالـ bacterial fermentation. Microbial
+
+6
+00:00:21,440 --> 00:00:24,340
+metabolic processes are complex, but they permit the
+
+7
+00:00:24,340 --> 00:00:27,700
+microbiologist to distinguish among microorganisms
+
+8
+00:00:27,700 --> 00:00:32,050
+grown in culture. بقولي: العمليات الأيضية التي تقوم
+
+9
+00:00:32,050 --> 00:00:36,690
+فيها البكتيريا معقدة، لكنها تسمح لأخصائي الـ
+
+10
+00:00:36,690 --> 00:00:40,510
+microbiology أن يميز بين الميكروبات التي تنمو في
+
+11
+00:00:40,510 --> 00:00:44,470
+المزرعة. Many clinical pathogens can be identified
+
+12
+00:00:44,470 --> 00:00:47,830
+by manipulating pure cultures into media that
+
+13
+00:00:47,830 --> 00:00:52,950
+contain one or more specific biochemicals. بقولي: الـ
+
+14
+00:00:52,950 --> 00:00:57,950
+pathogens ممكن أن أقدر أعرفها من خلال أني
+
+15
+00:00:57,950 --> 00:01:03,760
+أزرعها في pure culture. وصلت العينة، أروح أنا أزرعها
+
+16
+00:01:03,760 --> 00:01:08,040
+بطريقة الـ quadrant. على سبيل المثال، كانت عينة  تيرين
+
+17
+00:01:08,040 --> 00:01:12,360
+روحت زرعتها بطريقة الـ quadrant، أحصل على isolated
+
+18
+00:01:12,360 --> 00:01:15,380
+colonies من خلال طريقة الـ quadrant، من خلال طريقة
+
+19
+00:01:15,380 --> 00:01:19,680
+الأربعة. هذه الـ isolated colonies آخذها وأعمل pure
+
+20
+00:01:19,680 --> 00:01:22,880
+culture. أروح على طبق أزرع هذه الـ isolated colonies
+
+21
+00:01:22,880 --> 00:01:27,160
+آخذها وأزرعها حتى أحصل على pure culture. هيك
+
+22
+00:01:27,160 --> 00:01:31,640
+أطمئن أن المزرعة سيكون أصلها خلية بكتيريا واحدة،
+
+23
+00:01:31,640 --> 00:01:35,620
+يعني أنا لما أريد أن أشتغل، سأشتغل على بكتيريا واحدة فقط،
+
+24
+00:01:35,620 --> 00:01:39,460
+ليس mixed population، عشان تكون نتائجي الـ
+
+25
+00:01:39,460 --> 00:01:43,920
+biochemical tests صحيحة. يبقى الـ pure culture مهمة جدًا
+
+26
+00:01:43,920 --> 00:01:51,000
+عشان أشتغل الفحوصات الكيميائية. أنا لما أزرع العينة
+
+27
+00:01:51,000 --> 00:01:54,520
+وأحصل على isolated colonies، بعدين أعمل pure
+
+28
+00:01:54,520 --> 00:01:58,400
+culture. آخذ من الـ pure culture وأشتغل فحوصات
+
+29
+00:01:58,400 --> 00:02:04,140
+كيميائية. الفحص الكيميائي ممكن يكون عبارة عن medium.
+
+30
+00:02:04,140 --> 00:02:07,520
+أروح أزرع فيه الـ pure culture في medium يحتوي على
+
+31
+00:02:07,520 --> 00:02:11,840
+one specific biochemical.  شو قصده بهذه الجملة، على
+
+32
+00:02:11,840 --> 00:02:16,860
+سبيل المثال، فحص الـ Simmons citrate أو فحص الستريت. أنا
+
+33
+00:02:16,860 --> 00:02:21,140
+أستخدم medium Simmons citrate agar. اسمها Simmons
+
+34
+00:02:21,140 --> 00:02:26,880
+citrate agar. هذا الـ medium فقط يكشف لي عن فحص
+
+35
+00:02:26,880 --> 00:02:30,000
+كيميائي واحد، وهو الستريت. هل البكتيريا عندها
+
+36
+00:02:30,000 --> 00:02:35,240
+القدرة على استهلاك الستريت أم لا. لكن في بعض أنواع
+
+37
+00:02:35,240 --> 00:02:39,240
+الـ media، لما أنا أزرعها، أزرع البكتيريا من الـ pure
+
+38
+00:02:39,240 --> 00:02:42,680
+culture في هذا الـ medium، يكشف لي عن more than one specific
+
+39
+00:02:42,680 --> 00:02:46,750
+biochemical. أكثر من فحص كيميائي. سنتعرف في chapter
+
+40
+00:02:46,750 --> 00:02:51,830
+الـ Single Media Multiple Tests على هذا الموضوع. أنه
+
+41
+00:02:51,830 --> 00:02:54,470
+ميديا، أنا أزرع البكتيريا في ميديا. هذه الميديا
+
+42
+00:02:54,470 --> 00:03:00,050
+تكشف لي عن أكثر من فحص كيميائي. كيف أنا أريد أن أستدل
+
+43
+00:03:00,050 --> 00:03:04,330
+أن التفاعل صار أم لا؟ بقول لنا من خلال نواتج التفاعل،
+
+44
+00:03:04,330 --> 00:03:09,230
+أشوف النتائج، ممكن بالعين المجردة، زي فحص الـ catalase. كنت
+
+45
+00:03:09,230 --> 00:03:14,360
+أرى الـ air bubbles. فحص الـ coagulase، برضه
+
+46
+00:03:14,360 --> 00:03:17,600
+ممكن أن أرى بالعين المجردة. كنت أرى الـ Clumpy
+
+47
+00:03:17,600 --> 00:03:24,660
+ممكن برضه أن أضيف reagent في الميديا. هذا الـ
+
+48
+00:03:24,660 --> 00:03:31,000
+reagent يتفاعل مع نواتج التفاعل أو يغير من لون
+
+49
+00:03:31,000 --> 00:03:34,960
+الميديا. على سبيل المثال، فحص الستريت. فحص الستريت
+
+50
+00:03:34,960 --> 00:03:38,960
+كان فيه pH indicator، وهو الـ Bromothymol Blue،
+
+51
+00:03:38,960 --> 00:03:45,560
+كان موجودًا من مكونات الميديا. لما التفاعل، لو
+
+52
+00:03:45,560 --> 00:03:48,580
+البكتيريا عندها القدرة على استهلاك الستريت، قلنا
+
+53
+00:03:48,580 --> 00:03:51,860
+سيتكون الـ final product، وهو الـ Ammonia، الـ
+
+54
+00:03:51,860 --> 00:03:56,660
+NH3، سيرفع الـ pH. في عندي pH indicator في الـ media،
+
+55
+00:03:56,660 --> 00:04:02,240
+سيتغير لون الـ media حسب طبعًا شو الـ pH indicator
+
+56
+00:04:02,240 --> 00:04:06,220
+وشو لونه في الـ acidic أو الـ basic حسب نواتج التفاعل.
+
+57
+00:04:06,220 --> 00:04:11,060
+كان في الستريت، ناتج التفاعل Ammonia. الـ pH
+
+58
+00:04:11,060 --> 00:04:15,680
+indicator في الـ basic كان لونه blue، يبقى سيتغير
+
+59
+00:04:15,680 --> 00:04:19,180
+لون الـ media من اللون الأخضر للون الأزرق. هيك أنا
+
+60
+00:04:19,180 --> 00:04:24,200
+أعرف أنه البكتيريا قدرت تستهلك الستريت. يبقى هي
+
+61
+00:04:24,200 --> 00:04:30,180
+ستريت positive. هذا بالنسبة للـ reagent أو الـ pH
+
+62
+00:04:30,180 --> 00:04:34,460
+indicator كان موجودًا في الـ media. ممكن أنا بعد الـ
+
+63
+00:04:34,460 --> 00:04:39,470
+incubation أضيف reagent يتفاعل مع نواتج التفاعل، زي
+
+64
+00:04:39,470 --> 00:04:43,250
+فحص الـ amylase. الـ amylase، كنا نضيف الـ iodine بعد
+
+65
+00:04:43,250 --> 00:04:47,250
+الـ incubation. يبقى نواتج التفاعل ممكن أن
+
+66
+00:04:47,250 --> 00:04:51,670
+أراها بالعين المجردة، زي فحص الـ catalase والـ coagulase. ممكن
+
+67
+00:04:51,670 --> 00:04:55,550
+أن أضيف pH indicator أو reagent يتفاعل مع
+
+68
+00:04:55,550 --> 00:04:58,510
+نواتج التفاعل. هذا reagent يكون موجودًا في الـ media
+
+69
+00:04:58,510 --> 00:05:03,740
+زي فحص الستريت، أو ممكن أن أضيف reagent بعد الـ
+
+70
+00:05:03,740 --> 00:05:08,300
+incubation. هذا زي فحص الـ amylase. هذا الـ reagent
+
+71
+00:05:08,300 --> 00:05:13,940
+سيتفاعل مع نواتج التفاعل، لأنه ممكن يكون الـ
+
+72
+00:05:13,940 --> 00:05:18,280
+reagent هذا يتكسر في الـ autoclave. عشان هيك أنا
+
+73
+00:05:18,280 --> 00:05:21,760
+أضيفه بعد الـ incubation. الـ biochemical reactions
+
+74
+00:05:21,760 --> 00:05:25,580
+that result from substrate metabolism can be determined
+
+75
+00:05:25,580 --> 00:05:28,700
+by relatively simple indicator reagents. بقول: كيف
+
+76
+00:05:28,700 --> 00:05:32,440
+أستدل على التفاعل من خلال simple indicator reagents؟
+
+77
+00:05:32,440 --> 00:05:35,520
+هذا الـ reagent ممكن يكون موجودًا في الـ medium زي فحص
+
+78
+00:05:35,520 --> 00:05:39,380
+الستريت، أو ممكن أنا أضيفه على المزرعة بعد الـ
+
+79
+00:05:39,380 --> 00:05:43,300
+incubation زي فحص الـ amylase. أو قلنا ممكن أن أرى
+
+80
+00:05:43,300 --> 00:05:48,560
+التفاعل بالعين المجردة، زي فحص الـ catalase والـ
+
+81
+00:05:48,560 --> 00:05:51,600
+coagulase. Some bacteria ferment carbohydrates,
+
+82
+00:05:52,800 --> 00:05:57,520
+producing acidic, alcohols, or gases as end products. بقول
+
+83
+00:05:57,520 --> 00:06:00,540
+لي أن بعض البكتيريا عندها القدرة على تخمير
+
+84
+00:06:00,540 --> 00:06:05,880
+السكريات، تنتج acid أو alcohol أو gas. يبقى نواتج
+
+85
+00:06:05,880 --> 00:06:10,880
+عملية الـ fermentation acid أو alcohol أو gas. بعض
+
+86
+00:06:10,880 --> 00:06:15,280
+أنواع الميكروبات تنتج acid with gas، وفي بعض آخر
+
+87
+00:06:15,280 --> 00:06:21,510
+تنتج الـ acid without gas. كيف سنميز البكتيريا؟
+
+88
+00:06:21,510 --> 00:06:24,370
+Many different species are distinguished on the
+
+89
+00:06:24,370 --> 00:06:28,250
+basis of the carbohydrates they do or don't attack,
+
+90
+00:06:28,250 --> 00:06:31,970
+as well as by the nature of the end products formed
+
+91
+00:06:31,970 --> 00:06:35,870
+during fermentation. بقول لي: أقدر أميز بين
+
+92
+00:06:35,870 --> 00:06:41,270
+الميكروبات من خلال أنواع الكربوهيدرات التي تستطيع
+
+93
+00:06:41,270 --> 00:06:47,360
+تخميرها. يبقى أقدر أميز بين الميكروبات من خلال نوع
+
+94
+00:06:47,360 --> 00:06:52,940
+الكربوهيدرات التي تقدر أن تخمرها. كافي يعني
+
+95
+00:06:52,940 --> 00:06:58,840
+قلنا أنّه من أنواع الكربوهيدرات الجلوكوز.
+
+96
+00:06:58,840 --> 00:07:02,280
+قلنا في بعض أنواع البكتيريا تقدر تخمر الجلوكوز.
+
+97
+00:07:02,280 --> 00:07:06,340
+Glucose fermenters زي الـ Enterobacteriaceae. في بعض أنواع
+
+98
+00:07:06,340 --> 00:07:09,720
+البكتيريا لا تقدر تخمر الجلوكوز. Non-glucose
+
+99
+00:07:09,720 --> 00:07:17,070
+fermenters، وهي الـ Pseudomonas. من خلال هذا الفحص أقدر
+
+100
+00:07:17,070 --> 00:07:20,870
+أن أميز الـ Pseudomonas عن الـ Enterobacteriaceae. قلنا
+
+101
+00:07:20,870 --> 00:07:23,690
+أن الـ Enterobacteriaceae تنقسم لـ lactose fermenters و
+
+102
+00:07:23,690 --> 00:07:28,630
+non-lactose fermenters. يبقى من خلال هذا الفحص أقدر
+
+103
+00:07:28,630 --> 00:07:32,650
+أميز عائلة الـ Enterobacteriaceae أو أن أقسمها
+
+104
+00:07:32,650 --> 00:07:37,280
+لمجموعات. يساعدني في التعريف. شيء ثاني من خلال الـ
+
+105
+00:07:37,280 --> 00:07:40,620
+end products التي تكونت خلال عملية الـ fermentation.
+
+106
+00:07:40,620 --> 00:07:44,380
+قلنا نواتج عملية الـ fermentation ممكن تكون acid
+
+107
+00:07:44,380 --> 00:07:49,080
+أو alcohol أو gas. ممكن تكون acid لوحدها، ممكن تكون
+
+108
+00:07:49,080 --> 00:07:55,220
+acid with gas. بكون أنا عارفة البكتيريا X تعمل نواتج عملية الـ fermentation acid without gas.
+
+109
+00:07:55,220 --> 00:07:59,140
+البكتيريا Y نواتج عملية الـ fermentation acid with
+
+110
+00:08:04,140 --> 00:08:08,880
+gas. يبقى من خلال نواتج الـ fermentation أقدر أني
+
+111
+00:08:08,880 --> 00:08:13,100
+أنا أتعرف على البكتيريا. The nature of end products
+
+112
+00:08:13,100 --> 00:08:17,140
+formed in amino acid metabolism also provides
+
+113
+00:08:17,140 --> 00:08:20,940
+information as to the identification of bacterial
+
+114
+00:08:20,940 --> 00:08:24,500
+species. بقولي: برضه من خلال الـ amino acid
+
+115
+00:08:24,500 --> 00:08:28,540
+metabolism ممكن أنها تقدم لي معلومات تساعدني في
+
+116
+00:08:28,540 --> 00:08:33,020
+تعريف الـ bacterial species. على سبيل المثال، في عندي
+
+117
+00:08:33,020 --> 00:08:37,300
+amino acid اسمه tryptophan. في بعض أنواع البكتيريا
+
+118
+00:08:37,300 --> 00:08:42,380
+عندها enzyme tryptophanase، تكسر tryptophan تمامًا،
+
+119
+00:08:42,380 --> 00:08:47,560
+وبحصل على نواتج، وهي Indole، Pyruvate، وأمونيا.
+
+120
+00:08:47,560 --> 00:08:53,440
+الممكن أن أستفيد طبعًا من الأمونيا كـ nitrogen
+
+121
+00:08:53,440 --> 00:08:57,660
+source. البيروفات فيه كمصدر للطاقة، كـ carbon source.
+
+122
+00:08:57,660 --> 00:09:02,860
+هذا من خلال اللي هو in vivo. In vitro ممكن أستغل هذا
+
+123
+00:09:02,860 --> 00:09:07,260
+ال enzyme عشان أعمله فحص كيميائي. أشوف هل البكتيريا
+
+124
+00:09:07,260 --> 00:09:10,900
+عندها enzyme tryptophanase أم لا. وهذا سيساعدني
+
+125
+00:09:10,900 --> 00:09:16,120
+في الـ identification. سنتعرف عليه بالتفصيل في الـ
+
+126
+00:09:16,120 --> 00:09:20,700
+chapter الذي سيأتي. في فحص الصبغة، test the
+
+127
+00:09:20,700 --> 00:09:23,740
+production of visible pigments distinguishes certain
+
+128
+00:09:23,740 --> 00:09:28,700
+types of bacteria. Pseudomonas aeruginosa وبعض أنواع الصبغات
+
+129
+00:09:28,700 --> 00:09:34,740
+تستخدم للتمييز بين أنواع البكتيريا. Pseudomonas تفرز
+
+130
+00:09:34,740 --> 00:09:39,440
+صبغة soluble pigment. يبقى Pseudomonas تفرز صبغة
+
+131
+00:09:39,440 --> 00:09:52,260
+soluble pigment. إيش
+
+132
+00:09:52,260 --> 00:09:57,180
+يعني soluble pigment؟ هي لونها أصفر أو أخضر. Soluble
+
+133
+00:09:57,180 --> 00:10:01,660
+pigment يعني بتلون الكولوني، وكمان تنتشر في الطبق.
+
+134
+00:10:01,660 --> 00:10:05,800
+يبقى أجد الطبق كله، الأجار مع الكولوني صار لونها
+
+135
+00:10:05,800 --> 00:10:10,220
+أخضر. لكن Serratia، بقولي insoluble pigment.
+
+136
+00:10:30,530 --> 00:10:34,030
+Serratia، insoluble pigment.  ما معنى insoluble
+
+137
+00:10:34,030 --> 00:10:38,270
+pigment؟ فقط سأجد لون الكولوني، لن تنتشر في
+
+138
+00:10:38,270 --> 00:10:42,210
+الطبق. لونها يكون أحمر. يبقى Pseudomonas soluble
+
+139
+00:10:42,210 --> 00:10:46,310
+pigment، ما معنى soluble pigment؟ يعني ستلون الكولوني
+
+140
+00:10:46,310 --> 00:10:50,810
+مع الطبق كامل باللون الأخضر أو الأصفر. Serratia insoluble
+
+141
+00:10:50,810 --> 00:10:55,710
+pigment، فقط ستلون الكولوني باللون الأحمر، ليس
+
+142
+00:10:55,710 --> 00:11:00,870
+الأجار، لأنها insoluble، لن تستطيع أن تذوب في المزرعة.
+
+143
+00:11:00,870 --> 00:11:03,990
+Working with a biological structure freshly
+
+144
+00:11:03,990 --> 00:11:08,190
+isolated from a clinical specimen, the microbiologist
+
+145
+00:11:08,190 --> 00:11:12,430
+uses a carefully selected Petri dish of special media
+
+146
+00:11:12,430 --> 00:11:16,370
+to identify the organism's outstanding biochemical
+
+147
+00:11:16,370 --> 00:11:21,280
+characteristics. بقول لي: إنه خلال شغلي في الـ Biochemical
+
+148
+00:11:21,280 --> 00:11:24,340
+tests أستخدم الـ pure culture، وقلنا لماذا pure
+
+149
+00:11:24,340 --> 00:11:27,880
+culture؟ لأن أصلها خلية بكتيريا واحدة، هيك أضمن أنّه
+
+150
+00:11:27,880 --> 00:11:32,380
+أنا أعرف بكتيريا واحدة فقط، وهي X. الـ fresh culture
+
+151
+00:11:32,380 --> 00:11:37,740
+لازم يكون، لأن الـ enzyme activity يقل مع مرور
+
+152
+00:11:37,740 --> 00:11:42,180
+الوقت، فيعطني false results. الـ microbiologist
+
+153
+00:11:42,180 --> 00:11:48,260
+يقول لي: يختار الـ media التي سيزرع عليها العينات
+
+154
+00:11:48,970 --> 00:11:53,470
+عيناتي على أي أساس؟ نحن نختار الـ media، بقول لنا
+
+155
+00:11:53,470 --> 00:11:58,050
+نختار الـ media حسب نوع العينة. الـ stool، لو وصلتني
+
+156
+00:11:58,050 --> 00:12:02,150
+عينة stool، ليست نفس الـ media التي سأزرع عليها عينة الـ
+
+157
+00:12:02,150 --> 00:12:06,270
+urine. عشان هيك أريد أن أجيب media فقط تنمي الـ
+
+158
+00:12:06,270 --> 00:12:09,330
+pathogens. الـ pathogens الموجودة في الـ stool تختلف عن
+
+159
+00:12:09,330 --> 00:12:12,670
+الموجودة في الـ urine. يعني يكون الـ common pathogens
+
+160
+00:12:12,670 --> 00:12:16,320
+in stool يختلف عن الـ common pathogens in urine.
+
+161
+00:12:16,320 --> 00:12:20,600
+فبالتالي أنا فقط أستخدم media تنمي الـ pathogens،
+
+162
+00:12:20,600 --> 00:12:27,340
+فأختار media حسب نوع العينة. ستتعرفون في المعمل الـ
+
+163
+00:12:27,340 --> 00:12:31,960
+diagnostic في الفصل القادم. كل عينة سيكون لها
+
+164
+00:12:31,960 --> 00:12:36,500
+chapter خاص. كيف أنا، أو شو الـ media التي سأزرع عليها
+
+165
+00:12:36,500 --> 00:12:37,720
+هذه العينة؟
+
+166
+00:12:46,580 --> 00:12:51,520
+Bacteria mainly produce ATP by: بقول لي أن البكتيريا
+
+167
+00:12:51,520 --> 00:12:56,060
+تنتج الـ ATP بثلاث عمليات. العملية الأولى الـ Aerobic
+
+168
+00:12:56,060 --> 00:13:00,200
+respiration. الثانية عملية الـ fermentation. الثالثة عملية
+
+169
+00:13:00,200 --> 00:13:03,560
+الـ Anaerobic respiration. سنتعرف على كل عملية
+
+170
+00:13:03,560 --> 00:13:09,600
+بالتفصيل. الـ Aerobic respiration، التنفس الهوائي، is an
+
+171
+00:13:09,600 --> 00:13:13,500
+oxidative process which uses oxygen as the final
+
+172
+00:13:13,500 --> 00:13:17,960
+electron acceptor. بقول: للتنفس الهوائي، البكتيريا
+
+173
+00:13:17,960 --> 00:13:21,960
+التي تقوم به، يجب أن تكون obligate aerobes، يجب أن تكون
+
+174
+00:13:21,960 --> 00:13:28,080
+هوائية إجبارية. Oxidative process، تستخدم الأكسجين
+
+175
+00:13:28,080 --> 00:13:31,800
+كـ final electron acceptor، المستقبل النهائي
+
+176
+00:13:31,800 --> 00:13:35,340
+للإلكترونات. يكون الأكسجين، الأكسجين يعتبر
+
+177
+00:13:35,340 --> 00:13:40,240
+
+223
+00:16:59,870 --> 00:17:04,630
+اللي يتم إنتاجها لكل جزيء جلوكوز بتختلف حسب
+
+224
+00:17:04,630 --> 00:17:08,430
+نوع المستقبل النهائي للإلكترونات. لو أنا استخدمت
+
+225
+00:17:08,430 --> 00:17:11,810
+حديد، كمية الطاقة بتختلف عن لو أنا استخدمت قابل
+
+226
+00:17:11,810 --> 00:17:18,080
+هذا بالنسبة للتنفس الهوائي (aerobic respiration)، كمية الطاقة
+
+227
+00:17:18,080 --> 00:17:22,580
+التي تنتج حسب المستقبل النهائي للإلكترونات. يبقى الـ
+
+228
+00:17:22,580 --> 00:17:25,280
+aerobic respiration بيستخدم جزيء غير عضوي
+
+229
+00:17:25,280 --> 00:17:30,440
+الأكسجين، كمية الطاقة من 36 إلى 38 ATP. الآن، aerobic
+
+230
+00:17:30,440 --> 00:17:34,660
+respiration بيستخدم جزيء غير عضوي زي الحديد،
+
+231
+00:17:34,660 --> 00:17:38,360
+المنجنيز، الكوبالت، النيترات، السلفات، كمية الطاقة حسب
+
+232
+00:17:38,360 --> 00:17:42,350
+المستقبل النهائي للإلكترونات. التخمُّر (fermentation)
+
+233
+00:17:42,350 --> 00:17:46,410
+بيستخدم جزيئات عضوية زي الكربوهيدرات، كمية
+
+234
+00:17:46,410 --> 00:17:51,810
+الطاقة فقط 2 ATP لكل جزيء جلوكوز. آخر جملة موجودة
+
+235
+00:17:51,810 --> 00:17:55,330
+في هذه الشريحة (slide) بقول: "All fermentative bacteria can
+
+236
+00:17:55,330 --> 00:17:58,530
+ferment the simplest sugar"، اللي هو الجلوكوز أو
+
+237
+00:17:58,530 --> 00:18:02,170
+الديجليكوز. كل البكتيريا اللي لها القدرة أنْ
+
+238
+00:18:02,170 --> 00:18:06,250
+تعمل التخمُّر (fermentation)، بقول بتقدر تعمل fermentation
+
+239
+00:18:06,250 --> 00:18:10,740
+لأبسط سكر اللي هو الجلوكوز. الجلوكوز قلنا
+
+240
+00:18:10,740 --> 00:18:15,580
+مونوساكاريد، يبقى هو سكر بسيط. فأي
+
+241
+00:18:15,580 --> 00:18:19,280
+بكتيريا بتقدر أنْ تعمل fermentation، هتكون بتعمل
+
+242
+00:18:19,280 --> 00:18:23,180
+fermentation لأبسط السكريات اللي هو الجلوكوز.
+
+243
+00:18:23,180 --> 00:18:27,800
+اللاكتوز قلنا ديساكاريد، اللي هو بيتكون من two
+
+244
+00:18:27,800 --> 00:18:33,800
+sugars. البكتيريا لو عملت تخمير لللاكتوز، بتكون في الأصل
+
+245
+00:18:33,800 --> 00:18:38,900
+هي عاملة تخمير للجلوكوز، لأن اللاكتوز يعتبر ديساكاريد،
+
+246
+00:18:39,420 --> 00:18:43,100
+سكر معقد. فأكيد بما أنها قدرت تعمل fermentation
+
+247
+00:18:43,100 --> 00:18:48,000
+للسكر المعقد، هتكون عملت fermentation للسكر
+
+248
+00:18:48,000 --> 00:18:52,640
+الأبسط منه اللي هو الجلوكوز المونوساكاريد. لكن العكس
+
+249
+00:18:52,640 --> 00:18:58,280
+مش صحيح. يعني البكتيريا لو ما قدرت تعمل تخمير لللاكتوز،
+
+250
+00:18:58,280 --> 00:19:01,700
+ممكن في الأصل تكون هي  جلوكوز fermenter.
+
+251
+00:19:06,660 --> 00:19:11,660
+الـ non-lactose fermenter، هي ما بتقدر تعمل تخمير لللاكتوز،
+
+252
+00:19:11,660 --> 00:19:17,200
+لكن قدرت تعمل تخمير للجلوكوز، للسكر البسيط. ممكن أنْ
+
+253
+00:19:17,200 --> 00:19:20,660
+تكون في الأصل هي non-lactose fermenter، وكمان non-
+
+254
+00:19:20,660 --> 00:19:24,500
+glucose fermenter، زي السودوموناس. السّودوموناس هي non-
+
+255
+00:19:24,500 --> 00:19:28,080
+glucose fermenter، فأكيد مش هتقدر تعمل تخمير
+
+256
+00:19:28,080 --> 00:19:32,320
+للسكر المعقد، ما قدرت تعمل تخمير للسكر البسيط، فشيء
+
+257
+00:19:32,320 --> 00:19:36,280
+طبيعي مش هتقدر تعمل تخمير للسكر المعقد اللاكتوز.
+
+258
+00:19:36,280 --> 00:19:40,520
+يبقى بقول: إن أي بكتيريا بتعمل fermentation
+
+259
+00:19:40,520 --> 00:19:44,940
+هتكون بتقدر تعمل fermentation لأبسط سكر،
+
+260
+00:19:44,940 --> 00:19:50,480
+اللي هو الجلوكوز. لو كانت عاملة تخمير لسكر معقد، بدي
+
+261
+00:19:50,480 --> 00:19:56,680
+أفهم بشكل بديهي أنْ هي قدرت تعمل تخمير للسكر
+
+262
+00:19:56,680 --> 00:20:02,320
+البسيط اللي هو الجلوكوز. لكن العكس مش صحيح، ممكن
+
+263
+00:20:02,320 --> 00:20:07,000
+أنها تكون تقدر تعمل، وممكن ما تكون تقدر تعمل
+
+264
+00:20:07,000 --> 00:20:12,320
+fermentation للجلوكوز، زي اللاكتوز. قلنا في البكتيريا المعوية
+
+265
+00:20:12,320 --> 00:20:16,100
+(Enterobacteriaceae)، في عندي non-lactose fermenter، لكن هي في الأصل
+
+266
+00:20:16,100 --> 00:20:20,260
+قدرت تعمل تخمير للجلوكوز. في عندي non-lactose fermenter زي
+
+267
+00:20:20,260 --> 00:20:28,340
+السودوموناس، لكن هي في الأصل ما بتقدر تعمل تخمير للجلوكوز. طيب،
+
+268
+00:20:28,340 --> 00:20:31,220
+كيف بدي أنا أعرف هل البكتيريا عملت fermentation
+
+269
+00:20:31,220 --> 00:20:35,040
+ولا لأ؟ هذا فحص اللي هو اختبار التخمُّر البكتيري (bacterial fermentation).
+
+270
+00:20:35,040 --> 00:20:38,830
+كيف أنا بدي أعمل هذا الفحص؟  مبادئ هذا الفحص،
+
+271
+00:20:38,830 --> 00:20:41,690
+قولنا أن البكتيريا اللي بتقدر تعمل fermentation
+
+272
+00:20:41,690 --> 00:20:45,950
+أكيد هي تشتغل على الركيزة (substrate) اللي هي
+
+273
+00:20:45,950 --> 00:20:50,070
+الكربوهيدرات. هنحط طبعاً جلوكوز اللي هو أبسط سكر
+
+274
+00:20:50,070 --> 00:20:53,910
+في الوسط (media). الكربوهيدرات، نواتج عملية الـ
+
+275
+00:20:53,910 --> 00:20:56,390
+fermentation. أكيد الـ fermentation هتشتغل على
+
+276
+00:20:56,390 --> 00:21:00,230
+الكربوهيدرات. نواتج العملية: حمض (acidic) أو كحول (alcohol)
+
+277
+00:21:00,230 --> 00:21:04,370
+أو غازات (gases). نواتج العملية: حمض (acid) أو
+
+278
+00:21:04,370 --> 00:21:07,980
+كحول (alcohol) أو غاز (gas). كيف طريقة الشغل؟ بدي أحضر
+
+279
+00:21:07,980 --> 00:21:12,680
+أنبوبة تخمير (fermentation tube). بتكون هي المرق (broth). مكونات هذه الـ
+
+280
+00:21:12,680 --> 00:21:18,060
+أنبوبة (tube): المرق المغذي (nutrient broth). المرق المغذي (nutrient broth). أي
+
+281
+00:21:18,060 --> 00:21:21,100
+وسط (media)، سواء الوسط المغذي (nutrient agar) أو الوسط المغذي (nutrient)
+
+282
+00:21:21,100 --> 00:21:25,200
+broth، بتحتوي على مصادر للتغذية عشان تقدر تنمو
+
+283
+00:21:25,200 --> 00:21:28,960
+البكتيريا. من مصادر التغذية، هي في عندي بيبتون كمصدر
+
+284
+00:21:28,960 --> 00:21:33,770
+للنيتروجين. قلنا في عندي ممكن كمان خلاصة لحم البقر (Beef Extract)
+
+285
+00:21:33,770 --> 00:21:40,290
+كمصدر للكربون (Carbon). فينول أحمر (Phenol Red). فينول أحمر (Phenol Red) BH
+
+286
+00:21:40,290 --> 00:21:48,530
+مؤشر (Indicator) بتركيز 0.00189%. BH Indicator، لأنه احنا
+
+287
+00:21:48,530 --> 00:21:52,450
+قلنا نواتج العملية حمض (acidic) أو كحول (alcohol)، فعشان أكشف عن
+
+288
+00:21:52,450 --> 00:21:57,060
+نواتج هذه أو نواتج التفاعل، اللي بستخدم BH Indicator.
+
+289
+00:21:57,060 --> 00:22:00,420
+من الباقي؟ BH Indicator اللي بستخدمه، اللي هو الـ
+
+290
+00:22:00,420 --> 00:22:06,220
+فينول أحمر (Phenol Red) بتركيز 0.00189%.
+
+291
+00:22:06,220 --> 00:22:10,440
+الركيزة (substrate) اللي هيشتغل عليها الإنزيم لازم تكون
+
+292
+00:22:10,440 --> 00:22:17,040
+في الوسط (media)، اللي هي الجلوكوز بنسبة 1.2%. يبقى فيه
+
+293
+00:22:17,040 --> 00:22:20,720
+جلوكوز، أكيد هيكون جلوكوز. طيب، احنا قلنا بدنا نكشف على
+
+294
+00:22:20,720 --> 00:22:25,510
+الحمض (acid) أو الكحول من خلال الفينول الأحمر (Phenol Red). طب، كيف بدي
+
+295
+00:22:25,510 --> 00:22:29,030
+أكشف على الغاز؟ بقول في عندي أنبوبة درهم (Durham tube)، هي اللي
+
+296
+00:22:29,030 --> 00:22:32,750
+هتكشف لي على نواتج الغاز (gas product)، بتكون موجودة في
+
+297
+00:22:32,750 --> 00:22:38,890
+الأنبوبة. بتكون
+
+298
+00:22:38,890 --> 00:22:42,430
+موجودة مقلوبة في الأنبوبة، بنحطها هنا.
+
+299
+00:22:50,370 --> 00:22:55,450
+هذه أنبوبة درهم (Durham tube)، بحطها بشكل مقلوب، هتقدر تكشف لي على
+
+300
+00:22:55,450 --> 00:23:00,730
+نواتج الغاز (gas product). طبعاً، أنا بحطها قبل ما أدخلها أوتوكلاف
+
+301
+00:23:00,730 --> 00:23:05,970
+(autoclave)، لأنه إذا كان موجود في عندي فقاعات هواء (air bubbles) في هذه
+
+302
+00:23:05,970 --> 00:23:09,270
+الأنبوبة (tube)، قبل الحضانة (incubation)، قبل أنْ أزرع الـ
+
+303
+00:23:09,270 --> 00:23:13,130
+أوتوكلاف (autoclave)، هيتخلص من هذه الفقاعات الهوائية (air bubbles) عشان ما يصير
+
+304
+00:23:13,130 --> 00:23:17,650
+في عندي أي نتيجة خاطئة (false result). لأنْ كيف النتيجة بتكون؟
+
+305
+00:23:17,650 --> 00:23:21,010
+بيكون عندي، إذا كانت هي قدرت تنتج غاز، بيكون في عندي
+
+306
+00:23:21,010 --> 00:23:25,450
+فقاعات هواء (air bubbles) في الأنبوبة (tube). عشان أمنع النتيجة الخاطئة (false)
+
+307
+00:23:25,450 --> 00:23:28,630
+positive result، لازم أني أنا أدخلها الأوتوكلاف (autoclave)
+
+308
+00:23:28,630 --> 00:23:31,270
+بعدين أني أنا أزرع، عشان إذا كان في عندي أي فقاعات هواء (air)
+
+309
+00:23:31,270 --> 00:23:36,090
+bubbles موجودة ما قبل الحضانة (incubation)، الأوتوكلاف (autoclave) أنا
+
+310
+00:23:36,090 --> 00:23:40,690
+أتخلص منه من خلال الأوتوكلاف (autoclave). هي يبقى مكونات الـ
+
+311
+00:23:40,690 --> 00:23:47,020
+الوسط (media) اللي فيه. نوردُ، الألوان تبعته، بقول لي: في non-
+
+312
+00:23:47,020 --> 00:23:50,380
+acidic، بيكون لونه أصفر (yellow). في الـ basic
+
+313
+00:23:50,380 --> 00:23:53,840
+بيكون لونه أحمر (red). في الـ neutral بيكون لونه برتقالي (orange). إذا
+
+314
+00:23:53,840 --> 00:23:59,740
+كان أقل من 6.8 بيكون حمضي (acidic). ما بين 6.8 و 8.4 بيكون الـ
+
+315
+00:23:59,740 --> 00:24:04,600
+متعادل (neutral). إذا كان أعلى من 8.4 بيكون قاعدي (basic)، بيكون لونه
+
+316
+00:24:04,600 --> 00:24:09,960
+أحمر (red). أعمل حضانة (incubation) للبكتيريا اللي أنا بدي أفحصها،
+
+317
+00:24:09,960 --> 00:24:15,260
+هل بتقدر تعمل fermentation ولا لأ؟ هاخد المرق (broth)، هاخد الـ
+
+318
+00:24:15,260 --> 00:24:20,800
+البكتيريا، وأعمل خلط (mix) في هذه الأنبوبة (tube). أعمل
+
+319
+00:24:20,800 --> 00:24:27,300
+حضانة (incubation) لمدة 24 ساعة على 37 درجة مئوية. بعد 24
+
+320
+00:24:27,300 --> 00:24:32,200
+ساعة، بقول النتائج بتكون كالتالي: إما هتتغير لون
+
+321
+00:24:32,200 --> 00:24:37,110
+الأنبوبة (tube) للون الأصفر (yellow)، أي يعني بقول: هذا معناه أن
+
+322
+00:24:37,110 --> 00:24:41,570
+البكتيريا قدرت تعمل fermentation للجلوكوز. نواتج
+
+323
+00:24:41,570 --> 00:24:45,730
+عملية الـ fermentation حمضية (acidic)، اتكون حمض، فبالتالي
+
+324
+00:24:45,730 --> 00:24:49,470
+هيعمل على خفض الرقم الهيدروجيني (pH)، وهيتحول اللون للون أصفر (yellow).
+
+325
+00:24:49,470 --> 00:24:53,090
+أصفر (yellow) زائد فقاعات هواء (air bubble). الفقاعات الهوائية (air bubble) هتكون في
+
+326
+00:24:53,090 --> 00:24:55,690
+أنبوبة درهم (Durham tube). بقول: أن البكتيريا قدرت تعمل
+
+327
+00:24:55,690 --> 00:24:59,330
+fermentation للجلوكوز. نواتج عملية الـ fermentation
+
+328
+00:24:59,330 --> 00:25:04,350
+حمضية (acid)، قلّلت الرقم الهيدروجيني (pH)، اتحول اللون للون أصفر. وبنفس
+
+329
+00:25:04,350 --> 00:25:07,210
+الوقت كمان، كان من نواتج عملية الـ fermentation
+
+330
+00:25:07,210 --> 00:25:11,850
+أنتجت غاز. كيف بدي أكشف عنه؟ من خلال أنبوبة درهم (Durham tube).
+
+331
+00:25:11,850 --> 00:25:15,170
+هشوف فقاعات هواء (air bubbles) في أنبوبة درهم (Durham tube). يبقى هذه النتيجة
+
+332
+00:25:15,170 --> 00:25:19,470
+بتكون نواتج التفاعل أو نواتج الـ fermentation يكون
+
+333
+00:25:19,470 --> 00:25:25,850
+حمض مع غاز (acid with gas). لكن هذا حمض بدون غاز (acid without gas). برتقالي (Orange) زائد
+
+334
+00:25:25,850 --> 00:25:30,030
+فقاعات هواء (air bubbles). برتقالي (Orange). هلْ عملت fermentation ولا لأ؟ لأ،
+
+335
+00:25:30,030 --> 00:25:33,130
+لأن البرتقالي (orange) بيكون مُعبّر عن مُتَعادل (neutral). ما تكون عندي
+
+336
+00:25:33,130 --> 00:25:38,370
+النواتج حمضية (acidic product). طيب، كيف نتج الغاز عندي وهو من
+
+337
+00:25:38,370 --> 00:25:42,110
+نواتج عملية الـ fermentation؟ بيقول لي هنا، بيكون مش
+
+338
+00:25:42,110 --> 00:25:46,250
+مسكّر الأنبوبة منيح، بيكون الغاز من برّا، ما فيش عندي
+
+339
+00:25:46,250 --> 00:25:50,130
+تخمُّر (fermentation)، لكن الفقاعات الهوائية (air bubbles) اتكونت نتيجة
+
+340
+00:25:50,130 --> 00:25:56,070
+أن الأنبوبة (tube) كانت مش مسكّرة منيح. الأحمر (red)، اللون الأحمر
+
+341
+00:25:56,300 --> 00:25:58,920
+أكيد مش هيكون في عندي fermentation، لأن اللون
+
+342
+00:25:58,920 --> 00:26:05,560
+الأحمر موجود في الـ basic. في حال كان في عندي
+
+343
+00:26:05,560 --> 00:26:10,360
+قاعدي (alkaline)، ما أصبح في عندي fermentation. طيب، شو سبب
+
+344
+00:26:10,360 --> 00:26:14,560
+اللون الأحمر؟ بقول لي أنه يكون نتيجة نزع الأمين (deamination)
+
+345
+00:26:14,560 --> 00:26:18,140
+للببتون من مكونات الوسط (media). في عندي بيبتون،
+
+346
+00:26:18,140 --> 00:26:22,220
+هذا البيبتون، البكتيريا اللي ما قدرت تعمل
+
+347
+00:26:22,220 --> 00:26:25,880
+fermentation للجلوكوز، هتروح لمصادر ثانية، هتروح
+
+348
+00:26:25,880 --> 00:26:31,520
+للببتون، تعمل نزع أمين (deamination)، هتنزع الـ NH3.
+
+349
+00:26:31,520 --> 00:26:36,160
+نزع الأمين (Deamination)، أيش معناه؟ أنْها تنزع الأمونيا من البيبتون،
+
+350
+00:26:36,160 --> 00:26:41,140
+اللي هو الحمض الأميني (amino acid). الحمض الأميني (amino acid)، أحد اللي هو
+
+351
+00:26:41,140 --> 00:26:46,380
+مكوناته أو تركيبه (structure)، بيكون فيه ذرة NH3.
+
+352
+00:26:47,020 --> 00:26:50,260
+البكتيريا هتعمل نزع الأمين (deamination)، هتنزع الـ NH3،
+
+353
+00:26:50,260 --> 00:26:54,300
+الأمونيا، عشان تحصل على نيتروجين، تقدر تنمو
+
+354
+00:26:54,300 --> 00:26:58,820
+وتتكاثر. تتكون الـ NH3، أمونيا، الأمونيا قاعدية (alkaline)،
+
+355
+00:26:58,820 --> 00:27:05,080
+هرفع الرقم الهيدروجيني (pH)، وتتحول لون الوسط (media) للون أحمر. هيك
+
+356
+00:27:05,080 --> 00:27:08,680
+بنكون خلصنا اختبار التخمُّر البكتيري (bacterial fermentation). قلنا الـ
+
+357
+00:27:08,680 --> 00:27:11,480
+bacterial fermentation، عشان المبدأ (principle) أنّه
+
+358
+00:27:11,480 --> 00:27:15,520
+الكربوهيدرات (carbohydrate)، الجلوكوز، بيكون موجود في الوسط (media). لو
+
+359
+00:27:15,520 --> 00:27:18,300
+البكتيريا قدرت تعمل fermentation، النواتج هتكون
+
+360
+00:27:18,300 --> 00:27:22,700
+حمض (acid) أو كحول (alcohol) أو غاز (gas). كيف بدي أكشف عن الحمض (acid)؟ في
+
+361
+00:27:22,700 --> 00:27:26,160
+عندي مؤشر الرقم الهيدروجيني (pH indicator) اللي هو الفينول الأحمر (Phenol Red). كيف بدي أكشف
+
+362
+00:27:26,160 --> 00:27:29,640
+على الغاز (gas)؟ في عندي أنبوبة درهم (Durham tube)، بقدر أكشف على الـ
+
+363
+00:27:29,640 --> 00:27:36,020
+غاز (gas). عرفنا كل شيء، وتفسير هذه الألوان بالنسبة
+
+364
+00:27:36,020 --> 00:27:39,600
+للأهمية (Significant) لهذا الفحص. الأهمية (Significant) لهذا
+
+365
+00:27:39,600 --> 00:27:43,660
+الفحص، اللي هو هيميز بين اللي قلنا: الـ glucose
+
+366
+00:27:43,660 --> 00:27:48,090
+fermenter، بين موجب (positive) وسالب (negative). بين السّودوموناس والـ
+
+367
+00:27:48,090 --> 00:27:53,910
+البكتيريا المعوية (Enterobacteriaceae). كمان في عندي في الـ Enterobacteriaceae،
+
+368
+00:27:53,910 --> 00:27:56,690
+ممكن أنْ تُميز المُخمرّ لللاكتوز (lactose fermenter) عن الـ non-
+
+369
+00:27:56,690 --> 00:27:59,210
+lactose fermenter. هذا بالنسبة للأهمية (significant).
+
+370
+00:28:08,880 --> 00:28:11,960
+تخمُّر الكربوهيدرات (Fermentation of Carbohydrate). الكربوهيدرات (Carbohydrate) هي
+
+371
+00:28:11,960 --> 00:28:15,680
+ركائز كيميائية معقدة (complex chemical substrate) التي تعمل كمصدر
+
+372
+00:28:15,680 --> 00:28:19,880
+للطاقة (energy source). قلنا إن الكربوهيدرات (carbohydrate) هي معقدة، بتكون
+
+373
+00:28:19,880 --> 00:28:24,380
+مصدر للطاقة، وكمان مصدر للكربون (Carbon Source). لما تتكسر
+
+374
+00:28:24,380 --> 00:28:28,880
+بواسطة البكتيريا (bacteria) والخلايا الأخرى (other cells). أكيد أنا لما
+
+375
+00:28:28,880 --> 00:28:32,640
+تتكسر، بحصل عليه من خلالها طاقة، وبحصل على
+
+376
+00:28:32,640 --> 00:28:35,640
+مصدر كربون (Carbon Source). هذا المصدر الكربوني (Carbon Source) مهم لنموّ
+
+377
+00:28:35,640 --> 00:28:40,530
+الخلية، الخلية البكتيرية. مكونات الكربوهيدرات (Carbohydrate)،
+
+378
+00:28:40,630 --> 00:28:46,170
+كربون (Carbon)، هيدروجين (Hydrogen) وأكسجين (Oxygen). حسب هذه العلاقة، ممكن
+
+379
+00:28:46,170 --> 00:28:49,810
+أنْ يُصنّف إلى سكر (Sugar) أو نشا (Starch). السكريات (Sugar) اللي
+
+380
+00:28:49,810 --> 00:28:52,970
+هي الم
+
+445
+00:33:14,370 --> 00:33:18,880
+Fermentation acid lowers the pH of the media بقول لي
+
+446
+00:33:18,880 --> 00:33:22,160
+أن الـ acid بيعمل على خفض الـ pH في الـ media، فبالتالي
+
+447
+00:33:22,160 --> 00:33:26,720
+بيخلي الـ pH indicator يتحول للون الأصفر، لو
+
+448
+00:33:26,720 --> 00:33:30,280
+البكتيريا ما قدرت تخمر الـ carbohydrate، بقول لي أن
+
+449
+00:33:30,280 --> 00:33:34,300
+الـ media هيظل لونها أحمر أو هتعمل deamination لـ
+
+450
+00:33:34,300 --> 00:33:39,020
+البروتينات، هتنزع الأمونيا الـ NH3، الـ NH3 قلوي
+
+451
+00:33:39,020 --> 00:33:43,920
+فبالتالي هيتحول لون الـ media للون الأحمر، if gas
+
+452
+00:33:43,920 --> 00:33:48,700
+is produced as a by-product of fermentation، then
+
+453
+00:33:48,700 --> 00:33:52,040
+the Durham tube will have a bubble in it، بقول لي لو
+
+454
+00:33:52,040 --> 00:33:56,860
+اتكون غاز من الـ fermentation، أنا بقدر أكشف عليه من
+
+455
+00:33:56,860 --> 00:34:03,000
+خلال إني أنا بشوف air bubbles في الـ Durham tube، الـ
+
+456
+00:34:03,000 --> 00:34:05,800
+carbohydrate اللي ممكن إني أنا أكشف عنها: جلوكوز،
+
+457
+00:34:05,800 --> 00:34:09,260
+لاكتوز، ساكروز، بقول لي all carbohydrate test media
+
+458
+00:34:09,260 --> 00:34:13,160
+should be inoculated with a transfer loop، عشان
+
+459
+00:34:13,160 --> 00:34:16,700
+أعمل الفحص، باستخدم اللي هو الـ transfer loop هذا
+
+460
+00:34:16,700 --> 00:34:21,700
+بالنسبة للـ result لـ fermentation، ملاحظ في tube 1
+
+461
+00:34:21,700 --> 00:34:26,660
+و tube 3 تكون اللون الأصفر، يبقى في صار في عندي
+
+462
+00:34:26,660 --> 00:34:32,080
+fermentation لجلوكوز، اتكون أسيد، اتحول اللون للون
+
+463
+00:34:32,080 --> 00:34:37,040
+الأصفر، طبعًا نتيجة اللي هو الـ decrease للـ pH، ملاحظ
+
+464
+00:34:37,040 --> 00:34:42,380
+إنّه high، لونها أخف من high، هنتعرف كمان شوية إن في
+
+465
+00:34:42,380 --> 00:34:47,110
+بعض أنواع البكتيريا بتعمل fermentation للجلوكوز
+
+466
+00:34:47,110 --> 00:34:51,770
+وبتنتج كمية كبيرة من الـ acid، أو بتنتج أحماض بتعمل
+
+467
+00:34:51,770 --> 00:34:57,810
+على خفض البيئة مش بصورة كبيرة، فبالتالي بيكون اللون
+
+468
+00:34:57,810 --> 00:35:02,170
+غامق شوي، لكن ها نتكون acid، لكن مش بصورة كبيرة، في
+
+469
+00:35:02,170 --> 00:35:06,790
+عندي air bubbles، هاي الـ tube اللي بالنص تكون اللون
+
+470
+00:35:06,790 --> 00:35:09,570
+الـ red، يبقى ما صار في عندي fermentation، صار في
+
+471
+00:35:09,570 --> 00:35:15,620
+عندي deamination، لأ، النيجاتيف لفرمنتشن تيوب 1 و
+
+472
+00:35:15,620 --> 00:35:24,040
+3، بوزيتيف لفرمنتشن، طيب
+
+473
+00:35:24,040 --> 00:35:30,020
+هلأ لو عملت جرام ستين، وطلعت جرام نيجاتيف، بصلة
+
+474
+00:35:30,020 --> 00:35:32,920
+ماهو الخطوة الثانية؟ الخطوة الثانية إني أنا بدي
+
+475
+00:35:32,920 --> 00:35:36,400
+أعمل فحص الـ Oxidase، قلنا في المعمل السابق عن هذا
+
+476
+00:35:36,400 --> 00:35:39,490
+الموضوع، إذا طلعت positive، يبقى فيها عندي احتمال
+
+477
+00:35:39,490 --> 00:35:42,870
+Pseudomonas, Vibrio, Campylobacter, Helicobacter,
+
+478
+00:35:43,310 --> 00:35:46,450
+Aeromonas، قولنا هي Oxidase positive، لو كانت
+
+479
+00:35:46,450 --> 00:35:49,370
+negative، فيها عندي احتمال Enterobacteriaceae و
+
+480
+00:35:49,370 --> 00:35:56,310
+Acinetobacter، طيب عشان أفرق بين هدول الـ positive،
+
+481
+00:35:56,550 --> 00:35:58,930
+oxidase positive، oxidase negative، إذا طلعت
+
+482
+00:35:58,930 --> 00:36:00,630
+Oxidase positive
+
+483
+00:36:03,420 --> 00:36:06,240
+يبقى عندي احتمال، قولنا الـ Vibrio, Campylobacter,
+
+484
+00:36:06,460 --> 00:36:09,260
+Helicobacter, Aeromonas و Pseudomonas، لو طلعت أكسيداز
+
+485
+00:36:09,260 --> 00:36:13,380
+negative، انتيروبكترياسي، أسينيتوباكتر، عشان أفرق الـ
+
+486
+00:36:13,380 --> 00:36:16,960
+positive، بقول لي بتعملي فحص الجلوكوز fermentation، لو
+
+487
+00:36:16,960 --> 00:36:20,200
+طلعت negative، قولنا Pseudomonas obligate aerobic
+
+488
+00:36:20,200 --> 00:36:25,840
+فبالتالي أكيد هتكون non-glucose fermenter، لو طلعت
+
+489
+00:36:25,840 --> 00:36:29,040
+positive، يبقى في عندي أربع احتمالات، Vibrio,
+
+490
+00:36:29,160 --> 00:36:33,060
+Campylobacter, Helicobacter, Aeromonas، بالنسبة لأ
+
+491
+00:36:33,060 --> 00:36:36,160
+الأكسيداز negative، عشان أفرق الـ Enterobacteriaceae و
+
+492
+00:36:36,160 --> 00:36:38,660
+الـ Acinetobacter، برضه بعمل فحص الجلوكوز
+
+493
+00:36:38,660 --> 00:36:41,320
+fermentation، لو طلعت positive يبقى هي
+
+494
+00:36:41,320 --> 00:36:43,740
+Enterobacteriaceae، الـ Enterobacteriaceae تعتبر
+
+495
+00:36:43,740 --> 00:36:47,600
+facultative and aerobic، كلنا facultative and
+
+496
+00:36:47,600 --> 00:36:51,060
+aerobic، بكتيريا بتقدر تعمل fermentation، لو طلعت
+
+497
+00:36:51,060 --> 00:36:55,160
+negative، يبقى هي Acinetobacter، برضه الـ انتيرو
+
+498
+00:36:55,160 --> 00:36:58,460
+بكترياسي، قلنا بنصنفها لمجموعتين، اللي هي lactose
+
+499
+00:36:58,460 --> 00:37:01,780
+fermenter و non-lactose fermenter، ممكن إني أنا أعمل
+
+500
+00:37:01,780 --> 00:37:06,020
+هذا الفحص، عشان أقدر إني أنا أسمي الـ انتيرو بكترياسي
+
+501
+00:37:06,020 --> 00:37:10,420
+لمجموعات، وهذا هيساعدني في التعريف، كيف فحص الـ
+
+502
+00:37:10,420 --> 00:37:14,680
+lactose fermenter، هنتعرف عليه في الـ differential
+
+503
+00:37:14,680 --> 00:37:19,140
+media، من خلال المقنكي، المقنكي هي تعتبر
+
+504
+00:37:19,140 --> 00:37:22,440
+differential media، هتقدر تتميز اللي lactose و non-
+
+505
+00:37:22,440 --> 00:37:26,870
+lactose fermenter، يبقى هي حسب النوع الـ
+
+506
+00:37:26,870 --> 00:37:30,010
+carbohydrate، أنا الـ type of carbohydrate، قدرت إن
+
+507
+00:37:30,010 --> 00:37:34,970
+أنا أعمل identification للبكتيريا، هيك بنكون خلصنا
+
+508
+00:37:34,970 --> 00:37:38,770
+أول فحص اللي هو الـ bacterial fermentation، هلأ
+
+509
+00:37:38,770 --> 00:37:42,810
+هنتعرف على فحص الـ methyl red، Voges-Proskauer، وهو
+
+510
+00:37:42,810 --> 00:37:44,850
+بيتعلق برضه في الـ fermentation
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/yt25unVl2bQ_raw.json b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/yt25unVl2bQ_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..13f6308fa0c1a17dc13008bd0530024e15f7658b
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/yt25unVl2bQ_raw.json
@@ -0,0 +1 @@
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تأتي بـ Blast Candidate", "tokens": [6027, 39184, 7779, 836, 399, 23758, 30767, 1829, 6156, 5016, 9381, 2423, 39184, 669, 5088, 651, 23758, 2423, 39184, 1300, 559, 317, 39896, 2655, 5172, 995, 30241, 20449, 8717, 2407, 9307, 7435, 16712, 5172, 995, 30241, 5296, 33456, 3224, 3714, 43020, 7251, 30544, 2423, 39184, 1300, 559, 317, 23758, 4724, 36081, 4117, 3794, 2288, 8978, 2423, 39184, 6049, 905, 27995, 6225, 8592, 7649, 39896, 4117, 41850, 4724, 11242, 33911, 3224, 39182, 2423, 39184, 7779, 836, 399, 2423, 39184, 5480, 2423, 21292, 2407, 9122, 32640, 1829, 16373, 10632, 38392, 6055, 10721, 31371, 4724, 39184, 2177, 525, 20466, 327, 473], "avg_logprob": -0.34943182119215377, "compression_ratio": 1.619718309859155, "no_speech_prob": 6.878376007080078e-05, "words": [{"start": 303.44, "end": 303.74, "word": "الـ", "probability": 0.428955078125}, {"start": 303.74, "end": 304.48, "word": " Incubation", "probability": 0.8719075520833334}, {"start": 304.48, "end": 305.18, "word": " هذا", 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لتانية عملية ال fermentation بستخدم organic molecule كمستقبل نهائي للإلكترونات احنا قلنا في ال aerobic respiration بستخدم inorganic molecule اللي هو الأكسجين لكن في ال fermentation بستخدم organic molecule كمستقبل نهائي للإلكترونات زي مين في عندي organic molecule بقولي بشكل رئيسي الكربوهيدرات على سبيل المثال جليكوز، لاكتاز، مالتاز، ساكراز", "tokens": [3555, 6027, 1863, 35457, 3660, 5296, 2655, 7649, 10632, 6225, 42213, 10632, 2423, 43161, 4724, 14851, 9778, 40448, 10220, 15582, 9122, 2304, 14851, 4587, 36150, 8717, 3224, 16373, 1829, 24976, 28814, 23275, 2655, 2288, 11536, 9307, 1975, 5016, 8315, 12174, 1211, 8315, 8978, 2423, 257, 2032, 65, 299, 1597, 7611, 4724, 14851, 9778, 40448, 294, 12372, 299, 15582, 13672, 1829, 31439, 16247, 4117, 3794, 7435, 9957, 44381, 8978, 2423, 43161, 4724, 14851, 9778, 40448, 10220, 15582, 9122, 2304, 14851, 4587, 36150, 8717, 3224, 16373, 1829, 24976, 28814, 23275, 2655, 2288, 11536, 9307, 30767, 1829, 3714, 9957, 8978, 43242, 1829, 10220, 15582, 4724, 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بتصير فقط في البكتيريا احنا قولنا ال aerobic respiration فقط لازم تكون البكتيريا هوائية إجبارية في ال fermentation بتكون anaerobic بكتيريا بكتيريا لا هوائية وكمان الفكولتات في anaerobic", "tokens": [1829, 3555, 4587, 7578, 23758, 20666, 1863, 35457, 3660, 5296, 39184, 43161, 4724, 10721, 14851, 9778, 40448, 10220, 15582, 30767, 1829, 33251, 25513, 2407, 3224, 25708, 2288, 9307, 9122, 2304, 10632, 41950, 995, 28671, 38392, 6055, 29399, 7435, 281, 39202, 2423, 43161, 39894, 9381, 13546, 6156, 47432, 8978, 29739, 4117, 2655, 13546, 25528, 1975, 5016, 8315, 12174, 12610, 8315, 2423, 11207, 22234, 1597, 7611, 6156, 47432, 5296, 31377, 2304, 6055, 30544, 29739, 4117, 2655, 13546, 25528, 31439, 16373, 10632, 11933, 7435, 3555, 9640, 10632, 8978, 2423, 43161, 39894, 30544, 34178, 260, 22234, 4724, 4117, 2655, 13546, 25528, 4724, 4117, 2655, 13546, 25528, 20193, 31439, 16373, 10632, 4032, 24793, 7649, 27188, 4117, 12610, 2655, 9307, 8978, 34178, 260, 22234], "avg_logprob": -0.16885503976284957, 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"text": "اللي هي الهوائية الاختيارية ممكن انها تعمل fermentation and aerobic respiration is similar to aerobic respiration بقولي بتشبه ال aerobic respiration لأنها بتستخدم inorganic molecule لكن في ال aerobic respiration احنا قولنا بنستخدم inorganic molecule اللي هو ال A2 لكن هنا انا بستخدم inorganic molecule such as iron منجانيز", "tokens": [6027, 20292, 39896, 2423, 3224, 2407, 16373, 10632, 2423, 47283, 31371, 9640, 10632, 3714, 43020, 16472, 11296, 6055, 25957, 1211, 43161, 293, 11207, 22234, 1597, 7611, 307, 2531, 281, 11207, 22234, 1597, 7611, 4724, 39648, 1829, 39894, 8592, 3555, 3224, 2423, 11207, 22234, 1597, 7611, 5296, 33456, 11296, 39894, 14851, 9778, 40448, 294, 12372, 299, 15582, 44381, 8978, 2423, 11207, 22234, 1597, 7611, 1975, 5016, 8315, 12174, 12610, 8315, 44945, 14851, 9778, 40448, 294, 12372, 299, 15582, 13672, 1829, 31439, 2423, 316, 17, 44381, 34105, 1975, 8315, 4724, 14851, 9778, 40448, 294, 12372, 299, 15582, 1270, 382, 6497, 3714, 1863, 7435, 7649, 1829, 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مش صحيح ممكن أنها تكون تقدر تعمل وممكن ما تكون تقدر تعمل fermentation للجليكوز زي اللكتوز", "tokens": [1211, 2407, 25961, 2655, 6225, 10943, 37977, 6055, 9778, 2304, 13546, 5296, 3794, 37983, 20449, 28543, 4724, 16254, 5551, 5172, 16095, 4724, 8592, 28820, 4724, 16254, 3224, 1829, 14739, 3224, 39896, 12174, 3215, 43500, 6055, 25957, 1211, 6055, 9778, 2304, 13546, 24976, 3794, 37983, 29739, 3794, 1829, 9566, 13672, 1829, 31439, 25724, 20292, 4117, 2407, 11622, 44381, 18863, 4117, 3794, 37893, 20328, 5016, 1829, 5016, 3714, 43020, 14739, 11296, 6055, 30544, 6055, 28543, 2288, 6055, 25957, 1211, 4032, 2304, 43020, 19446, 6055, 30544, 6055, 28543, 2288, 6055, 25957, 1211, 43161, 24976, 7435, 20292, 4117, 2407, 11622, 30767, 1829, 13672, 4117, 2655, 2407, 11622], "avg_logprob": -0.12567607628611419, "compression_ratio": 1.8882978723404256, "no_speech_prob": 2.205371856689453e-06, "words": [{"start": 1187.34, "end": 1187.66, "word": "لو", "probability": 0.871337890625}, {"start": 1187.66, 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"probability": 0.949462890625}, {"start": 1202.32, "end": 1202.58, "word": " أنها", "probability": 0.729248046875}, {"start": 1202.58, "end": 1202.94, "word": " تكون", "probability": 0.97265625}, {"start": 1202.94, "end": 1203.52, "word": " تقدر", "probability": 0.9944661458333334}, {"start": 1203.52, "end": 1204.04, "word": " تعمل", "probability": 0.9881184895833334}, {"start": 1204.04, "end": 1204.78, "word": " وممكن", "probability": 0.8707682291666666}, {"start": 1204.78, "end": 1205.06, "word": " ما", "probability": 0.89404296875}, {"start": 1205.06, "end": 1205.9, "word": " تكون", "probability": 0.979248046875}, {"start": 1205.9, "end": 1206.4, "word": " تقدر", "probability": 0.9952799479166666}, {"start": 1206.4, "end": 1207.0, "word": " تعمل", "probability": 0.9884440104166666}, {"start": 1207.0, "end": 1207.8, "word": " fermentation", "probability": 0.966796875}, {"start": 1207.8, "end": 1209.54, "word": " للجليكوز", "probability": 0.8950602213541666}, {"start": 1209.54, "end": 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{"start": 1545.51, "end": 1546.11, "word": " مصر", "probability": 0.5481770833333334}, {"start": 1546.11, "end": 1546.25, "word": " في", "probability": 0.86083984375}, {"start": 1546.25, "end": 1546.47, "word": " عندي", "probability": 0.932373046875}, {"start": 1546.47, "end": 1546.89, "word": " fermentation", "probability": 0.982421875}, {"start": 1546.89, "end": 1547.99, "word": " لكن", "probability": 0.9345703125}, {"start": 1547.99, "end": 1548.27, "word": " ال", "probability": 0.87158203125}, {"start": 1548.27, "end": 1548.43, "word": " air", "probability": 0.63720703125}, {"start": 1548.43, "end": 1548.75, "word": " bubbles", "probability": 0.8291015625}, {"start": 1548.75, "end": 1549.31, "word": " اتكون", "probability": 0.8987630208333334}, {"start": 1549.31, "end": 1550.13, "word": " لنتيجة", "probability": 0.86318359375}, {"start": 1550.13, "end": 1550.33, "word": " ان", "probability": 0.67724609375}, {"start": 1550.33, "end": 1550.49, "word": " ال", "probability": 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Deamination ايش معناها انها تنزع الامانيا البيبتون اللى هو ال amino acid ال amino acid احد اللى هو مكوناته او ال structure سبعته بيكون فيه اندى N-H3", "tokens": [6027, 3555, 4117, 2655, 13546, 25528, 13672, 7578, 19446, 28543, 43500, 6055, 25957, 1211, 43161, 24976, 7435, 20292, 4117, 2407, 11622, 8032, 2655, 32887, 5016, 32767, 9381, 18513, 2288, 6055, 7649, 10632, 8032, 2655, 32887, 5016, 24976, 21292, 3555, 2655, 11536, 6055, 25957, 1211, 368, 335, 2486, 8032, 2655, 1863, 11622, 3615, 2423, 39184, 426, 12, 39, 18, 1346, 335, 2486, 1975, 1829, 8592, 20449, 8315, 11296, 16472, 11296, 6055, 1863, 11622, 3615, 2423, 10943, 7649, 25528, 2423, 21292, 3555, 2655, 11536, 13672, 7578, 31439, 2423, 24674, 8258, 2423, 24674, 8258, 1975, 24401, 13672, 7578, 31439, 3714, 4117, 2407, 8315, 47395, 1975, 2407, 2423, 3877, 8608, 3555, 34268, 3224, 4724, 1829, 30544, 8978, 3224, 16472, 3215, 7578, 426, 12, 39, 18], "avg_logprob": -0.21606045704884608, "compression_ratio": 1.7887931034482758, 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الأمونيا عشان تحصل على نيتروجين سارس تقدر تنمو وتتكثر اتكون الـ NH3 أمونيا الأمونيا الالكالاين هيرفع الـ pH وتتحول لون ال ميديا للون أحمر هيك بنكون خلصنا ال bacterial fermentation قولنا ال bacterial fermentation عشان ال principle انه carbohydrate الشيليوكاز بيكون موجود في الميديا", "tokens": [6027, 3555, 4117, 2655, 13546, 25528, 8032, 2655, 25957, 1211, 2423, 39184, 1346, 335, 2486, 8032, 2655, 1863, 11622, 3615, 2423, 39184, 31118, 18, 16247, 2304, 11536, 25528, 6225, 8592, 7649, 6055, 5016, 36520, 15844, 8717, 36081, 2288, 29245, 9957, 8608, 9640, 3794, 6055, 28543, 2288, 6055, 1863, 2304, 2407, 34683, 2655, 4117, 49115, 1975, 2655, 30544, 2423, 39184, 31118, 18, 5551, 2304, 11536, 25528, 16247, 2304, 11536, 25528, 2423, 6027, 4117, 6027, 995, 9957, 39896, 28480, 3615, 2423, 39184, 21677, 34683, 2655, 5016, 12610, 5296, 11536, 2423, 3714, 1829, 16254, 995, 5296, 1211, 11536, 5551, 5016, 29973, 39896, 4117, 44945, 30544, 16490, 1211, 9381, 8315, 2423, 35632, 43161, 12174, 12610, 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{"start": 1653.44, "end": 1653.74, "word": " هذه", "probability": 0.8251953125}, {"start": 1653.74, "end": 1654.46, "word": " الألوان", "probability": 0.995361328125}, {"start": 1654.46, "end": 1656.02, "word": " بالنسبة", "probability": 0.9718017578125}, {"start": 1656.02, "end": 1656.26, "word": " للـ", "probability": 0.533935546875}, {"start": 1656.26, "end": 1657.02, "word": " Significant", "probability": 0.64697265625}, {"start": 1657.02, "end": 1657.52, "word": " لهذا", "probability": 0.9501953125}, {"start": 1657.52, "end": 1658.24, "word": " الفحص", "probability": 0.9514973958333334}, {"start": 1658.24, "end": 1658.52, "word": " الـ", "probability": 0.395751953125}, {"start": 1658.52, "end": 1659.06, "word": " Significant", "probability": 0.89404296875}, {"start": 1659.06, "end": 1659.6, "word": " لهذا", "probability": 0.990478515625}, {"start": 1659.6, "end": 1660.28, "word": " الفحص", "probability": 0.98828125}, {"start": 1660.28, "end": 1660.5, "word": " اللي", "probability": 0.8408203125}, {"start": 1660.5, "end": 1660.88, "word": " هو", "probability": 0.95263671875}, {"start": 1660.88, "end": 1661.9, "word": " هيميز", "probability": 0.8795166015625}, {"start": 1661.9, "end": 1662.28, "word": " بين", "probability": 0.96484375}, {"start": 1662.28, "end": 1662.7, "word": " اللي", "probability": 0.6541748046875}, {"start": 1662.7, "end": 1663.14, "word": " قلناه", "probability": 0.8182373046875}, {"start": 1663.14, "end": 1663.2, "word": " ال", "probability": 0.4189453125}, {"start": 1663.2, "end": 1663.66, "word": " gelicose", "probability": 0.530517578125}, {"start": 1663.66, "end": 1664.38, "word": " fermenter", "probability": 0.77294921875}], "temperature": 1.0}, {"id": 63, "seek": 167921, "start": 1664.95, "end": 1679.21, "text": "بين positive و negative بين الصدومونس وال انتيرو بكترياسي كمان في اندي في ال انتيرو بكترياسي ممكن انها تميز ال lactose fermenter عن ال non lactose fermenter هذا بالنسبة ل significant", "tokens": [3555, 9957, 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{"start": 1710.68, "end": 1711.2, "word": " خلالها", "probability": 0.9876708984375}, {"start": 1711.2, "end": 1711.62, "word": " علاقة", "probability": 0.6837565104166666}, {"start": 1711.62, "end": 1711.94, "word": " طاقة", "probability": 0.9928385416666666}, {"start": 1711.94, "end": 1712.04, "word": " و", "probability": 0.8134765625}, {"start": 1712.04, "end": 1712.38, "word": " بحصل", "probability": 0.9256184895833334}, {"start": 1712.38, "end": 1712.64, "word": " على", "probability": 0.7880859375}, {"start": 1712.64, "end": 1713.1, "word": " Carbon", "probability": 0.62353515625}, {"start": 1713.1, "end": 1713.5, "word": " Source", "probability": 0.94189453125}, {"start": 1713.5, "end": 1713.7, "word": " هذا", "probability": 0.90673828125}, {"start": 1713.7, "end": 1713.86, "word": " الـ", "probability": 0.806640625}, {"start": 1713.86, "end": 1714.12, "word": " Carbon", "probability": 0.86328125}, {"start": 1714.12, "end": 1714.46, "word": " Source", "probability": 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"probability": 0.4794921875}, {"start": 1723.99, "end": 1725.21, "word": " العلاقة", "probability": 0.91552734375}, {"start": 1725.21, "end": 1726.17, "word": " ممكن", "probability": 0.833251953125}, {"start": 1726.17, "end": 1726.57, "word": " أنه", "probability": 0.51025390625}, {"start": 1726.57, "end": 1727.05, "word": " يكون", "probability": 0.965087890625}, {"start": 1727.05, "end": 1727.69, "word": " صنفه", "probability": 0.828369140625}, {"start": 1727.69, "end": 1727.89, "word": " إلى", "probability": 0.8876953125}, {"start": 1727.89, "end": 1728.21, "word": " Sugar", "probability": 0.71923828125}, {"start": 1728.21, "end": 1728.65, "word": " or", "probability": 0.693359375}, {"start": 1728.65, "end": 1729.19, "word": " Starch", "probability": 0.7454833984375}, {"start": 1729.19, "end": 1729.39, "word": " الـ", "probability": 0.369140625}, {"start": 1729.39, "end": 1729.61, "word": " Sugar", "probability": 0.79052734375}, {"start": 1729.61, "end": 1729.81, "word": " اللي", "probability": 0.6153564453125}, {"start": 1729.81, "end": 1730.11, "word": " هي", "probability": 0.90185546875}, {"start": 1730.11, "end": 1730.37, "word": " الـ", "probability": 0.703369140625}, {"start": 1730.37, "end": 1731.63, "word": " Monodiosaccharide،", "probability": 0.6493966238839286}, {"start": 1731.63, "end": 1731.79, "word": " الـ", "probability": 0.667236328125}, {"start": 1731.79, "end": 1732.21, "word": " Starch", "probability": 0.927734375}, {"start": 1732.21, "end": 1732.69, "word": " قولنا", "probability": 0.7052408854166666}, {"start": 1732.69, "end": 1732.97, "word": " الـ", "probability": 0.701171875}, {"start": 1732.97, "end": 1734.21, "word": " Polysaccharide", "probability": 0.9015625}], "temperature": 1.0}, {"id": 66, "seek": 176420, "start": 1736.08, "end": 1764.2, "text": "facultative anaerobic and anaerobic bacteria are capable of fermentation قولت أنا عملية ال fermentation بتقدر اللي هي ال facultative anaerobic and anaerobic bacteria قولنا ال anaerobic respiration فقط ال obligate anaerobic هي اللي بتقدر تعمل ال anaerobic respiration لكن ال fermentation ال anaerobic bacteria و ال facultative anaerobic bacteria بتقدر تعمل fermentation", "tokens": [69, 326, 723, 1166, 34178, 260, 22234, 293, 34178, 260, 22234, 11763, 366, 8189, 295, 43161, 12174, 12610, 2655, 41850, 6225, 42213, 10632, 2423, 43161, 39894, 28543, 2288, 13672, 1829, 39896, 2423, 44137, 1166, 34178, 260, 22234, 293, 34178, 260, 22234, 11763, 12174, 12610, 8315, 2423, 34178, 260, 22234, 1597, 7611, 6156, 47432, 2423, 9270, 473, 34178, 260, 22234, 39896, 13672, 1829, 39894, 28543, 2288, 6055, 25957, 1211, 2423, 34178, 260, 22234, 1597, 7611, 44381, 2423, 43161, 2423, 34178, 260, 22234, 11763, 4032, 2423, 44137, 1166, 34178, 260, 22234, 11763, 39894, 28543, 2288, 6055, 25957, 1211, 43161], "avg_logprob": -0.1497927279192574, "compression_ratio": 2.4611111111111112, "no_speech_prob": 2.980232238769531e-07, "words": [{"start": 1736.0800000000002, "end": 1737.1200000000001, "word": "facultative", "probability": 0.66131591796875}, {"start": 1737.1200000000001, "end": 1738.16, "word": " anaerobic", "probability": 0.8232421875}, {"start": 1738.16, "end": 1738.52, "word": " and", "probability": 0.650390625}, {"start": 1738.52, "end": 1739.44, "word": " anaerobic", "probability": 0.9620768229166666}, {"start": 1739.44, "end": 1739.96, "word": " bacteria", "probability": 0.93408203125}, {"start": 1739.96, "end": 1740.24, "word": " are", "probability": 0.92578125}, {"start": 1740.24, "end": 1740.5, "word": " capable", "probability": 0.8671875}, {"start": 1740.5, "end": 1740.74, "word": " of", "probability": 0.97802734375}, {"start": 1740.74, "end": 1741.34, "word": " fermentation", "probability": 0.89111328125}, {"start": 1741.34, "end": 1741.74, "word": " قولت", "probability": 0.7823893229166666}, {"start": 1741.74, "end": 1741.94, "word": " أنا", "probability": 0.42529296875}, {"start": 1741.94, "end": 1742.34, "word": " عملية", "probability": 0.97607421875}, 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"probability": 0.59375}, {"start": 1755.16, "end": 1755.58, "word": " anaerobic", "probability": 0.9666341145833334}, {"start": 1755.58, "end": 1756.24, "word": " respiration", "probability": 0.945068359375}, {"start": 1756.24, "end": 1756.74, "word": " لكن", "probability": 0.91796875}, {"start": 1756.74, "end": 1757.06, "word": " ال", "probability": 0.9140625}, {"start": 1757.06, "end": 1757.8, "word": " fermentation", "probability": 0.9765625}, {"start": 1757.8, "end": 1758.56, "word": " ال", "probability": 0.71875}, {"start": 1758.56, "end": 1759.22, "word": " anaerobic", "probability": 0.9739583333333334}, {"start": 1759.22, "end": 1759.88, "word": " bacteria", "probability": 0.9423828125}, {"start": 1759.88, "end": 1760.52, "word": " و", "probability": 0.8623046875}, {"start": 1760.52, "end": 1760.78, "word": " ال", "probability": 0.556640625}, {"start": 1760.78, "end": 1761.4, "word": " facultative", "probability": 0.97705078125}, {"start": 1761.4, "end": 1762.08, "word": " 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"word": " صار", "probability": 0.912353515625}, {"start": 2108.07, "end": 2108.17, "word": " في", "probability": 0.9697265625}, {"start": 2108.17, "end": 2108.43, "word": " عندي", "probability": 0.9072265625}, {"start": 2108.43, "end": 2108.87, "word": " fermentation", "probability": 0.96923828125}, {"start": 2108.87, "end": 2109.45, "word": " صار", "probability": 0.9306640625}, {"start": 2109.45, "end": 2109.57, "word": " في", "probability": 0.96533203125}, {"start": 2109.57, "end": 2109.85, "word": " عندي", "probability": 0.9658203125}, {"start": 2109.85, "end": 2110.57, "word": " deamination", "probability": 0.7308756510416666}, {"start": 2110.57, "end": 2110.93, "word": " لأ", "probability": 0.8525390625}, {"start": 2110.93, "end": 2111.03, "word": " ال", "probability": 0.64892578125}], "temperature": 1.0}, {"id": 80, "seek": 213694, "start": 2112.52, "end": 2136.94, "text": "نجاتيف لفرمنتشن تيوب وان و ثري بوزيتيف لفرمنتشن طيب هلأ لو عملت إجرام استان و طلعت إجرام نيجاتيف بصلة ماهو الخطوة التانية الخطوة التانية أني أنا بدي أعمل فحص ال Oxidase قلنا في المعمل السابق عن هذا الموضوع", "tokens": [1863, 7435, 9307, 1829, 5172, 5296, 5172, 2288, 2304, 29399, 8592, 1863, 6055, 1829, 37746, 36764, 1863, 4032, 38637, 16572, 4724, 2407, 11622, 36081, 33911, 5296, 5172, 2288, 2304, 29399, 8592, 1863, 23032, 1829, 3555, 8032, 1211, 10721, 45164, 6225, 42213, 2655, 11933, 7435, 2288, 10943, 44713, 7649, 4032, 23032, 1211, 34268, 11933, 7435, 2288, 10943, 8717, 1829, 7435, 9307, 33911, 4724, 36520, 3660, 19446, 3224, 2407, 33962, 9566, 2407, 3660, 16712, 7649, 10632, 33962, 9566, 2407, 3660, 16712, 7649, 10632, 14739, 1829, 41850, 4724, 16254, 5551, 25957, 1211, 6156, 5016, 9381, 2423, 16489, 327, 651, 12174, 1211, 8315, 8978, 9673, 25957, 1211, 21136, 16758, 4587, 18871, 23758, 9673, 2407, 11242, 45367], "avg_logprob": -0.3202433670516563, "compression_ratio": 1.8865979381443299, "no_speech_prob": 2.0265579223632812e-06, "words": [{"start": 2112.52, "end": 2113.44, "word": 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2126.4, "word": " إجرام", "probability": 0.83551025390625}, {"start": 2126.4, "end": 2127.02, "word": " استان", "probability": 0.443359375}, {"start": 2127.02, "end": 2127.82, "word": " و", "probability": 0.92626953125}, {"start": 2127.82, "end": 2128.26, "word": " طلعت", "probability": 0.8712565104166666}, {"start": 2128.26, "end": 2128.88, "word": " إجرام", "probability": 0.9859619140625}, {"start": 2128.88, "end": 2129.46, "word": " نيجاتيف", "probability": 0.7302734375}, {"start": 2129.46, "end": 2130.02, "word": " بصلة", "probability": 0.68994140625}, {"start": 2130.02, "end": 2130.62, "word": " ماهو", "probability": 0.726318359375}, {"start": 2130.62, "end": 2131.1, "word": " الخطوة", "probability": 0.942626953125}, {"start": 2131.1, "end": 2131.62, "word": " التانية", "probability": 0.9617513020833334}, {"start": 2131.62, "end": 2132.06, "word": " الخطوة", "probability": 0.8829345703125}, {"start": 2132.06, "end": 2132.34, "word": " التانية", "probability": 0.9853515625}, {"start": 2132.34, "end": 2132.58, "word": " أني", "probability": 0.522705078125}, {"start": 2132.58, "end": 2132.72, "word": " أنا", "probability": 0.463623046875}, {"start": 2132.72, "end": 2132.92, "word": " بدي", "probability": 0.70947265625}, {"start": 2132.92, "end": 2133.24, "word": " أعمل", "probability": 0.9388020833333334}, {"start": 2133.24, "end": 2133.62, "word": " فحص", "probability": 0.9842122395833334}, {"start": 2133.62, "end": 2133.74, "word": " ال", "probability": 0.84814453125}, {"start": 2133.74, "end": 2134.32, "word": " Oxidase", "probability": 0.5455729166666666}, {"start": 2134.32, "end": 2134.76, "word": " قلنا", "probability": 0.8304036458333334}, {"start": 2134.76, "end": 2134.96, "word": " في", "probability": 0.96826171875}, {"start": 2134.96, "end": 2135.4, "word": " المعمل", "probability": 0.9402669270833334}, {"start": 2135.4, "end": 2135.94, "word": " السابق", "probability": 0.97705078125}, {"start": 2135.94, "end": 2136.1, "word": " عن", "probability": 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انتيروبكترياسي أسينيتوباكتر عشان أفرق ال positive بقولي بتعملي فحص الجليكوز fermentation لو طلعت negative قولنا Sodomonas obligate aerobic فبالتالي أكيد هتكون non-gelicose fermenter لو طلعت positive يبقى في عندي أربع احتمالات Vibrio, Campylobacter, Helicobacter, Aeromonas", "tokens": [1829, 3555, 4587, 7578, 18871, 16254, 1975, 33753, 2304, 6027, 12174, 12610, 8315, 2423, 39184, 691, 897, 6584, 11, 9189, 88, 752, 65, 14125, 11, 6128, 299, 996, 14125, 11, 32459, 24488, 296, 4032, 42059, 24488, 296, 45164, 23032, 1211, 34268, 5551, 4117, 3794, 3215, 31377, 3671, 16472, 31371, 32887, 3555, 4117, 2655, 16572, 32277, 1829, 5551, 3794, 9957, 36081, 37746, 995, 4117, 2655, 2288, 6225, 8592, 7649, 5551, 5172, 2288, 4587, 2423, 3353, 4724, 39648, 1829, 39894, 25957, 20292, 6156, 5016, 9381, 25724, 20292, 4117, 2407, 11622, 43161, 45164, 23032, 1211, 34268, 3671, 12174, 12610, 8315, 42059, 24488, 296, 9270, 473, 11207, 22234, 6156, 3555, 6027, 2655, 6027, 1829, 5551, 4117, 25708, 8032, 2655, 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0.9563802083333334}, {"start": 2178.24, "end": 2179.0, "word": " Sodomonas", "probability": 0.91064453125}, {"start": 2179.0, "end": 2179.76, "word": " obligate", "probability": 0.826171875}, {"start": 2179.76, "end": 2180.2, "word": " aerobic", "probability": 0.860595703125}, {"start": 2180.2, "end": 2180.74, "word": " فبالتالي", "probability": 0.981201171875}, {"start": 2180.74, "end": 2181.06, "word": " أكيد", "probability": 0.9104817708333334}, {"start": 2181.06, "end": 2181.76, "word": " هتكون", "probability": 0.9498697916666666}, {"start": 2181.76, "end": 2182.76, "word": " non", "probability": 0.445068359375}, {"start": 2182.76, "end": 2184.38, "word": "-gelicose", "probability": 0.6732177734375}, {"start": 2184.38, "end": 2185.04, "word": " fermenter", "probability": 0.502685546875}, {"start": 2185.04, "end": 2185.3, "word": " لو", "probability": 0.94482421875}, {"start": 2185.3, "end": 2185.84, "word": " طلعت", "probability": 0.9964192708333334}, {"start": 2185.84, "end": 2186.54, "word": " positive", "probability": 0.92822265625}, {"start": 2186.54, "end": 2187.08, "word": " يبقى", "probability": 0.983642578125}, {"start": 2187.08, "end": 2187.22, "word": " في", "probability": 0.8154296875}, {"start": 2187.22, "end": 2187.52, "word": " عندي", "probability": 0.822509765625}, {"start": 2187.52, "end": 2187.88, "word": " أربع", "probability": 0.8367513020833334}, {"start": 2187.88, "end": 2188.54, "word": " احتمالات", "probability": 0.9728515625}, {"start": 2188.54, "end": 2189.04, "word": " Vibrio,", "probability": 0.9371744791666666}, {"start": 2189.16, "end": 2189.9, "word": " Campylobacter,", "probability": 0.95947265625}, {"start": 2190.04, "end": 2190.76, "word": " Helicobacter,", "probability": 0.95458984375}, {"start": 2190.88, "end": 2191.48, "word": " Aeromonas", "probability": 0.96826171875}], "temperature": 1.0}, {"id": 83, "seek": 221376, "start": 2192.26, "end": 2213.76, "text": "بالنسبة لأ الأكسداز negative عشان أفرق الـ Enterobacteriase و الـ Acinetobacter برضه بعمل فحص الجليكوز fermentation لو طلعت positive يبقى هي Enterobacteriase الـ Enterobacteriase تعتبر facultative and aerobic كلنا facultative and aerobic بكتيريا بتقدر تعمل fermentation لو طلعت negative يبقى هي Acinetobacter", "tokens": [3555, 6027, 1863, 35457, 3660, 5296, 10721, 16247, 4117, 3794, 3215, 31377, 3671, 6225, 8592, 7649, 5551, 5172, 2288, 4587, 2423, 39184, 10399, 996, 14125, 4609, 68, 4032, 2423, 39184, 5097, 21370, 996, 14125, 4724, 43042, 3224, 4724, 25957, 1211, 6156, 5016, 9381, 25724, 20292, 4117, 2407, 11622, 43161, 45164, 23032, 1211, 34268, 3353, 7251, 3555, 4587, 7578, 39896, 10399, 996, 14125, 4609, 68, 2423, 39184, 10399, 996, 14125, 4609, 68, 6055, 34268, 26890, 44137, 1166, 293, 11207, 22234, 28242, 8315, 44137, 1166, 293, 11207, 22234, 4724, 4117, 2655, 13546, 25528, 39894, 28543, 2288, 6055, 25957, 1211, 43161, 45164, 23032, 1211, 34268, 3671, 7251, 3555, 4587, 7578, 39896, 5097, 21370, 996, 14125], "avg_logprob": -0.24419248420580297, "compression_ratio": 1.8266666666666667, "no_speech_prob": 2.2172927856445312e-05, "words": [{"start": 2192.26, "end": 2192.84, "word": "بالنسبة", "probability": 0.96064453125}, {"start": 2192.84, "end": 2193.06, "word": " لأ", "probability": 0.767822265625}, {"start": 2193.06, "end": 2193.74, "word": " الأكسداز", "probability": 0.66376953125}, {"start": 2193.74, "end": 2194.28, "word": " negative", "probability": 0.351806640625}, {"start": 2194.28, "end": 2194.7, "word": " عشان", "probability": 0.9591471354166666}, {"start": 2194.7, "end": 2195.1, "word": " أفرق", "probability": 0.8807373046875}, {"start": 2195.1, "end": 2195.26, "word": " الـ", "probability": 0.5390625}, {"start": 2195.26, "end": 2196.08, "word": " Enterobacteriase", "probability": 0.5928466796875}, {"start": 2196.08, "end": 2196.16, "word": " و", "probability": 0.68017578125}, {"start": 2196.16, "end": 2196.28, "word": " الـ", "probability": 0.5255126953125}, {"start": 2196.28, "end": 2196.92, "word": " Acinetobacter", "probability": 0.7998046875}, {"start": 2196.92, "end": 2197.4, "word": " برضه", "probability": 0.7496744791666666}, {"start": 2197.4, "end": 2197.74, "word": " بعمل", "probability": 0.9347330729166666}, {"start": 2197.74, "end": 2198.08, "word": " فحص", "probability": 0.9871419270833334}, {"start": 2198.08, "end": 2198.66, "word": " الجليكوز", "probability": 0.754296875}, {"start": 2198.66, "end": 2199.22, "word": " fermentation", "probability": 0.94482421875}, {"start": 2199.22, "end": 2199.86, "word": " لو", "probability": 0.83056640625}, {"start": 2199.86, "end": 2200.24, "word": " طلعت", "probability": 0.9910481770833334}, {"start": 2200.24, "end": 2200.74, "word": " positive", "probability": 0.89794921875}, {"start": 2200.74, "end": 2201.14, "word": " يبقى", "probability": 0.9647216796875}, {"start": 2201.14, "end": 2201.32, "word": " هي", "probability": 0.5517578125}, {"start": 2201.32, "end": 2202.4, "word": " Enterobacteriase", "probability": 0.921875}, {"start": 2202.4, "end": 2202.56, "word": " الـ", "probability": 0.5274658203125}, {"start": 2202.56, "end": 2203.34, "word": " Enterobacteriase", "probability": 0.95927734375}, {"start": 2203.34, "end": 2203.74, "word": " تعتبر", "probability": 0.90380859375}, {"start": 2203.74, "end": 2204.68, "word": " facultative", "probability": 0.80859375}, {"start": 2204.68, "end": 2205.12, "word": " and", "probability": 0.76953125}, {"start": 2205.12, "end": 2205.52, "word": " aerobic", "probability": 0.489105224609375}, {"start": 2205.52, "end": 2206.02, "word": " كلنا", "probability": 0.7222900390625}, {"start": 2206.02, "end": 2207.26, "word": " facultative", "probability": 0.63720703125}, {"start": 2207.26, "end": 2207.6, "word": " and", "probability": 0.94580078125}, {"start": 2207.6, "end": 2207.92, "word": " aerobic", "probability": 0.928955078125}, {"start": 2207.92, "end": 2208.56, "word": " بكتيريا", "probability": 0.836474609375}, {"start": 2208.56, "end": 2208.98, "word": " بتقدر", "probability": 0.9607747395833334}, {"start": 2208.98, "end": 2209.3, "word": " تعمل", "probability": 0.98583984375}, {"start": 2209.3, "end": 2209.86, "word": " fermentation", "probability": 0.98486328125}, {"start": 2209.86, "end": 2210.68, "word": " لو", "probability": 0.90576171875}, {"start": 2210.68, "end": 2211.06, "word": " طلعت", "probability": 0.9939778645833334}, {"start": 2211.06, "end": 2211.46, "word": " negative", "probability": 0.95458984375}, {"start": 2211.46, "end": 2212.26, "word": " يبقى", "probability": 0.967041015625}, {"start": 2212.26, "end": 2212.58, "word": " هي", "probability": 0.86865234375}, {"start": 2212.58, "end": 2213.76, "word": " Acinetobacter", "probability": 0.9505615234375}], "temperature": 1.0}, {"id": 84, "seek": 224367, "start": 2214.32, "end": 2243.68, "text": "برضه ال انتيرو بكترياسي قلنا بنصنفها لمجموعتان اللي هي lactose fermenter و non lactose fermenter ممكن ان انا اعمل هذا الفحص عشان اقدر ان انا اسم ال انتيرو بكترياسي لمجموعات وهذا هيساعدني في التعريف كيف فحص ال lactose fermenter هنتعرف عليه في ال differential media من خلال المقنكي المقنكي هي تعتبر differential media هتقدر تتميز اللي lactose و non lactose fermenter", "tokens": [3555, 43042, 3224, 2423, 16472, 31371, 32887, 4724, 4117, 2655, 16572, 32277, 1829, 12174, 1211, 8315, 44945, 9381, 1863, 5172, 11296, 32767, 7435, 2304, 45367, 2655, 7649, 13672, 1829, 39896, 34042, 541, 38300, 260, 4032, 2107, 34042, 541, 38300, 260, 3714, 43020, 16472, 1975, 8315, 1975, 25957, 1211, 23758, 27188, 5016, 9381, 6225, 8592, 7649, 1975, 28543, 2288, 16472, 1975, 8315, 24525, 2304, 2423, 16472, 31371, 32887, 4724, 4117, 2655, 16572, 32277, 1829, 32767, 7435, 2304, 45367, 9307, 37037, 15730, 39896, 3794, 995, 22488, 22653, 8978, 16712, 3615, 16572, 5172, 9122, 33911, 6156, 5016, 9381, 2423, 34042, 541, 38300, 260, 8032, 29399, 3615, 28480, 47356, 8978, 2423, 15756, 3021, 9154, 16490, 1211, 6027, 9673, 4587, 1863, 4117, 1829, 9673, 4587, 1863, 4117, 1829, 39896, 6055, 34268, 26890, 15756, 3021, 8032, 2655, 28543, 2288, 6055, 39237, 1829, 11622, 13672, 1829, 34042, 541, 4032, 2107, 34042, 541, 38300, 260], "avg_logprob": -0.17958192144696777, "compression_ratio": 2.1589147286821704, "no_speech_prob": 3.635883331298828e-06, "words": [{"start": 2214.32, "end": 2214.8, "word": "برضه", "probability": 0.683837890625}, {"start": 2214.8, "end": 2214.88, "word": " ال", "probability": 0.80712890625}, {"start": 2214.88, "end": 2215.16, "word": " انتيرو", "probability": 0.5665690104166666}, {"start": 2215.16, "end": 2215.78, "word": " بكترياسي", "probability": 0.755859375}, {"start": 2215.78, "end": 2216.16, "word": " قلنا", "probability": 0.7726236979166666}, {"start": 2216.16, "end": 2216.86, "word": " بنصنفها", "probability": 0.85400390625}, {"start": 2216.86, "end": 2217.58, "word": " لمجموعتان", "probability": 0.8634440104166666}, {"start": 2217.58, "end": 2217.72, "word": " اللي", "probability": 0.843017578125}, {"start": 2217.72, "end": 2217.92, "word": " هي", "probability": 0.88232421875}, {"start": 2217.92, "end": 2218.46, "word": " lactose", "probability": 0.87109375}, {"start": 2218.46, "end": 2219.0, "word": " fermenter", "probability": 0.74169921875}, {"start": 2219.0, "end": 2219.14, "word": " و", "probability": 0.93798828125}, {"start": 2219.14, "end": 2219.32, "word": " non", "probability": 0.74560546875}, {"start": 2219.32, "end": 2219.84, "word": " lactose", "probability": 0.79248046875}, {"start": 2219.84, "end": 2220.6, "word": " fermenter", "probability": 0.95166015625}, {"start": 2220.6, "end": 2220.92, "word": " ممكن", "probability": 0.970947265625}, {"start": 2220.92, "end": 2221.1, "word": " ان", "probability": 0.388916015625}, {"start": 2221.1, "end": 2221.34, "word": " انا", "probability": 0.72119140625}, {"start": 2221.34, "end": 2221.78, "word": " اعمل", "probability": 0.9352213541666666}, {"start": 2221.78, "end": 2222.16, "word": " هذا", "probability": 0.91064453125}, {"start": 2222.16, "end": 2222.68, "word": " الفحص", "probability": 0.92138671875}, {"start": 2222.68, "end": 2223.16, "word": " عشان", "probability": 0.9853515625}, {"start": 2223.16, "end": 2223.96, "word": " اقدر", "probability": 0.87255859375}, {"start": 2223.96, "end": 2224.08, "word": " ان", "probability": 0.9384765625}, {"start": 2224.08, "end": 2224.58, "word": " انا", "probability": 0.74609375}, {"start": 2224.58, "end": 2224.8, "word": " اسم", "probability": 0.652587890625}, {"start": 2224.8, "end": 2225.06, "word": " ال", "probability": 0.95654296875}, {"start": 2225.06, "end": 2225.36, "word": " انتيرو", "probability": 0.7997233072916666}, {"start": 2225.36, "end": 2226.02, "word": " بكترياسي", "probability": 0.980712890625}, {"start": 2226.02, "end": 2226.66, "word": " لمجموعات", "probability": 0.94169921875}, {"start": 2226.66, "end": 2226.94, "word": " وهذا", "probability": 0.81103515625}, {"start": 2226.94, "end": 2227.68, "word": " هيساعدني", "probability": 0.9490234375}, {"start": 2227.68, "end": 2228.4, "word": " في", "probability": 0.93408203125}, {"start": 2228.4, "end": 2229.24, "word": " التعريف", "probability": 0.98876953125}, {"start": 2229.24, "end": 2229.88, "word": " كيف", "probability": 0.95263671875}, {"start": 2229.88, "end": 2230.3, "word": " فحص", "probability": 0.8701171875}, {"start": 2230.3, "end": 2230.42, "word": " ال", "probability": 0.662109375}, {"start": 2230.42, "end": 2230.86, "word": " lactose", "probability": 0.97607421875}, {"start": 2230.86, "end": 2231.54, "word": " fermenter", "probability": 0.96044921875}, {"start": 2231.54, "end": 2232.62, "word": " هنتعرف", "probability": 0.9361572265625}, {"start": 2232.62, "end": 2232.98, "word": " عليه", "probability": 0.63671875}, {"start": 2232.98, "end": 2233.36, "word": " في", "probability": 0.91943359375}, {"start": 2233.36, "end": 2233.76, "word": " ال", "probability": 0.9326171875}, {"start": 2233.76, "end": 2234.68, "word": " differential", "probability": 0.87451171875}, {"start": 2234.68, "end": 2235.48, "word": " media", "probability": 0.94189453125}, {"start": 2235.48, "end": 2236.02, "word": " من", "probability": 0.97607421875}, {"start": 2236.02, "end": 2236.48, "word": " خلال", "probability": 0.9947916666666666}, {"start": 2236.48, "end": 2237.22, "word": " المقنكي", "probability": 0.649072265625}, {"start": 2237.22, "end": 2238.34, "word": " المقنكي", "probability": 0.95537109375}, {"start": 2238.34, "end": 2238.66, "word": " هي", "probability": 0.9052734375}, {"start": 2238.66, "end": 2239.14, "word": " تعتبر", "probability": 0.9793294270833334}, {"start": 2239.14, "end": 2239.7, "word": " differential", "probability": 0.880859375}, {"start": 2239.7, "end": 2240.24, "word": " media", "probability": 0.9482421875}, {"start": 2240.24, "end": 2240.78, "word": " هتقدر", "probability": 0.9796142578125}, {"start": 2240.78, "end": 2241.22, "word": " تتميز", "probability": 0.7967529296875}, {"start": 2241.22, "end": 2241.4, "word": " اللي", "probability": 0.6884765625}, {"start": 2241.4, "end": 2242.1, "word": " lactose", "probability": 0.6630859375}, {"start": 2242.1, "end": 2242.24, "word": " و", "probability": 0.92431640625}, {"start": 2242.24, "end": 2242.44, "word": " non", "probability": 0.80078125}, {"start": 2242.44, "end": 2243.06, "word": " lactose", "probability": 0.935791015625}, {"start": 2243.06, "end": 2243.68, "word": " fermenter", "probability": 0.958740234375}], "temperature": 1.0}, {"id": 85, "seek": 226485, "start": 2244.31, "end": 2264.85, "text": "يبقى هي حسب النوع الـ carbohydrate أنا الـ type of carbohydrate اقدرت ان انا اعمل identification للبكتيريا هيك بنكون خلصنا اول فحص اللي هو ال bacterial fermentation هلأ هنتعرف على فحص ال methyl red vucus بسكوار وهو بيتعلق برضه في ال fermentation", "tokens": [1829, 3555, 4587, 7578, 39896, 11331, 35457, 28239, 45367, 2423, 39184, 47048, 41850, 2423, 39184, 2010, 295, 47048, 1975, 28543, 43500, 16472, 1975, 8315, 1975, 25957, 1211, 22065, 24976, 3555, 4117, 2655, 13546, 25528, 39896, 4117, 44945, 30544, 16490, 1211, 9381, 8315, 1975, 12610, 6156, 5016, 9381, 13672, 1829, 31439, 2423, 35632, 43161, 8032, 1211, 10721, 8032, 29399, 3615, 28480, 15844, 6156, 5016, 9381, 2423, 48441, 2182, 9732, 1149, 4724, 3794, 4117, 2407, 9640, 37037, 2407, 4724, 36081, 30241, 4587, 4724, 43042, 3224, 8978, 2423, 43161], "avg_logprob": -0.23293821530780573, "compression_ratio": 1.536480686695279, "no_speech_prob": 1.1920928955078125e-07, "words": [{"start": 2244.31, "end": 2245.01, "word": "يبقى", "probability": 0.768646240234375}, {"start": 2245.01, "end": 2245.31, "word": " هي", "probability": 0.375}, {"start": 2245.31, "end": 2245.71, "word": " حسب", "probability": 0.956298828125}, {"start": 2245.71, "end": 2246.31, "word": " النوع", "probability": 0.8779296875}, {"start": 2246.31, "end": 2246.87, "word": " الـ", "probability": 0.57763671875}, {"start": 2246.87, "end": 2247.45, "word": " carbohydrate", "probability": 0.60791015625}, {"start": 2247.45, "end": 2247.99, "word": " أنا", "probability": 0.576171875}, {"start": 2247.99, "end": 2248.29, "word": " الـ", "probability": 0.77783203125}, {"start": 2248.29, "end": 2248.47, "word": " type", "probability": 0.62548828125}, {"start": 2248.47, "end": 2248.63, "word": " of", "probability": 0.95751953125}, {"start": 2248.63, "end": 2249.29, "word": " carbohydrate", "probability": 0.9189453125}, {"start": 2249.29, "end": 2249.83, "word": " اقدرت", "probability": 0.85009765625}, {"start": 2249.83, "end": 2250.01, "word": " ان", "probability": 0.75390625}, {"start": 2250.01, "end": 2250.37, "word": " انا", "probability": 0.809814453125}, {"start": 2250.37, "end": 2251.37, "word": " اعمل", "probability": 0.9685872395833334}, {"start": 2251.37, "end": 2252.39, "word": " identification", "probability": 0.900390625}, {"start": 2252.39, "end": 2253.81, "word": " للبكتيريا", "probability": 0.85595703125}, {"start": 2253.81, "end": 2254.13, "word": " هيك", "probability": 0.6773681640625}, {"start": 2254.13, "end": 2254.41, "word": " بنكون", "probability": 0.93408203125}, {"start": 2254.41, "end": 2254.97, "word": " خلصنا", "probability": 0.98046875}, {"start": 2254.97, "end": 2255.29, "word": " اول", "probability": 0.839111328125}, {"start": 2255.29, "end": 2255.65, "word": " فحص", "probability": 0.9806315104166666}, {"start": 2255.65, "end": 2255.79, "word": " اللي", "probability": 0.879638671875}, {"start": 2255.79, "end": 2256.11, "word": " هو", "probability": 0.99072265625}, {"start": 2256.11, "end": 2256.33, "word": " ال", "probability": 0.9521484375}, {"start": 2256.33, "end": 2256.87, "word": " bacterial", "probability": 0.845703125}, {"start": 2256.87, "end": 2257.81, "word": " fermentation", "probability": 0.93994140625}, {"start": 2257.81, "end": 2258.77, "word": " هلأ", "probability": 0.7081705729166666}, {"start": 2258.77, "end": 2259.37, "word": " هنتعرف", "probability": 0.9901123046875}, {"start": 2259.37, "end": 2259.63, "word": " على", "probability": 0.9111328125}, {"start": 2259.63, "end": 2260.09, "word": " فحص", "probability": 0.9947916666666666}, {"start": 2260.09, "end": 2260.23, "word": " ال", "probability": 0.80859375}, {"start": 2260.23, "end": 2260.59, "word": " methyl", "probability": 0.154296875}, {"start": 2260.59, "end": 2261.27, "word": " red", "probability": 0.8896484375}, {"start": 2261.27, "end": 2261.71, "word": " vucus", "probability": 0.3743896484375}, {"start": 2261.71, "end": 2262.31, "word": " بسكوار", "probability": 0.707373046875}, {"start": 2262.31, "end": 2262.81, "word": " وهو", "probability": 0.708740234375}, {"start": 2262.81, "end": 2263.55, "word": " بيتعلق", "probability": 0.9063720703125}, {"start": 2263.55, "end": 2263.93, "word": " برضه", "probability": 0.9326171875}, {"start": 2263.93, "end": 2264.15, "word": " في", "probability": 0.9365234375}, {"start": 2264.15, "end": 2264.39, "word": " ال", "probability": 0.927734375}, {"start": 2264.39, "end": 2264.85, "word": " fermentation", "probability": 0.970703125}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 2270.307875, "duration_after_vad": 2179.7712499999925}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/yt25unVl2bQ_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/yt25unVl2bQ_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..a15d1784473a9c58acf6c26bdb63e53abbaaac15
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/yt25unVl2bQ_raw.srt
@@ -0,0 +1,2040 @@
+1
+00:00:01,960 --> 00:00:05,660
+السلام عليكم ورحمة الله وبركاته نستكمل معاكم
+
+2
+00:00:05,660 --> 00:00:09,700
+محاضرات مساقى الـ Microbiology العملي ولازلنا في
+
+3
+00:00:09,700 --> 00:00:13,200
+ال biochemical test اليوم موضوعنا هيكون عن
+
+4
+00:00:13,200 --> 00:00:17,240
+bacterial fermentation and methyl red vocaspar
+
+5
+00:00:17,240 --> 00:00:21,440
+هنبدأ بال bacterial fermentation microbial
+
+6
+00:00:21,440 --> 00:00:24,340
+metabolic process are complex but they permit the
+
+7
+00:00:24,340 --> 00:00:27,700
+microbiologist to distinguish among microorganism
+
+8
+00:00:27,700 --> 00:00:32,050
+grown in cultureبقولي العمليات الأيضية اللي بتقوم
+
+9
+00:00:32,050 --> 00:00:36,690
+فيها البكتيريا معقدة لكن بتسمح لأخصائي ال
+
+10
+00:00:36,690 --> 00:00:40,510
+microbiology انه يميز بين الميكروبات اللي بتنمو في
+
+11
+00:00:40,510 --> 00:00:44,470
+المزرعة many clinical pathogen can be identified
+
+12
+00:00:44,470 --> 00:00:47,830
+by manipulating pure culture into media that
+
+13
+00:00:47,830 --> 00:00:52,950
+contain one or more specific biochemicals بقولي ال
+
+14
+00:00:52,950 --> 00:00:57,950
+pathogen ممكن ان انا اقدر اعرفها من خلال اني
+
+15
+00:00:57,950 --> 00:01:03,760
+بزرعهافي pure culture وصلت العينة بروح انا بزراها
+
+16
+00:01:03,760 --> 00:01:08,040
+بطريقة ال quadrant على سبيل المثال كانت عينة تيرين
+
+17
+00:01:08,040 --> 00:01:12,360
+روحت زراعتها بطريقة ال quadrant بحصل على isolated
+
+18
+00:01:12,360 --> 00:01:15,380
+كلني من خلال طريقة ال quadrant من خلال طريقة
+
+19
+00:01:15,380 --> 00:01:19,680
+الأربعة هذه ال isolated كلني باخدها و بعمل pure
+
+20
+00:01:19,680 --> 00:01:22,880
+culture بروح على طبق بزراها هذه ال isolated كلني
+
+21
+00:01:22,880 --> 00:01:27,160
+باخدها و بزراها حتى احصل على pure culture هيك
+
+22
+00:01:27,160 --> 00:01:31,640
+باطمنإن المزرعة هيكون أصلها خلية بكتيريا واحدة
+
+23
+00:01:31,640 --> 00:01:35,620
+يعني أنا لما بدي أشتغل هشتغل على بكتيريا واحدة فقط
+
+24
+00:01:35,620 --> 00:01:39,460
+X مش mixed population عشان تكون نتائجي ال
+
+25
+00:01:39,460 --> 00:01:43,920
+biochemical test صح يبقى ال pure culture مهمة جدا
+
+26
+00:01:43,920 --> 00:01:51,000
+عشان أشتغل الفحوصات الكيميائية أنا لما أزرعالعينة
+
+27
+00:01:51,000 --> 00:01:54,520
+و احصل على isolator كله اني بعدين اعمل pure
+
+28
+00:01:54,520 --> 00:01:58,400
+culture باخد من ال pure culture و بشتغل فحوصات
+
+29
+00:01:58,400 --> 00:02:04,140
+كيميائية الفحص الكيميائي ممكن يكون عبارة عن media
+
+30
+00:02:04,140 --> 00:02:07,520
+بروح بزراحي ال pure culture في media بتحتوي على
+
+31
+00:02:07,520 --> 00:02:11,840
+one specific biochemical شو قصده بهذه الجملة على
+
+32
+00:02:11,840 --> 00:02:16,860
+سبيل المثال فحص السيمون سايتراتأو فحص السيطرات أنا
+
+33
+00:02:16,860 --> 00:02:21,140
+بستخدم media simon citrate agar media اسمها simon
+
+34
+00:02:21,140 --> 00:02:26,880
+citrate agar هذه ال media فقط بتكشفلي على فحص
+
+35
+00:02:26,880 --> 00:02:30,000
+كيميائي واحد اللي هو السيطرات هل البكتيريا عندها
+
+36
+00:02:30,000 --> 00:02:35,240
+القدرة على استهلاك السيطرات أو لأ لكن في بعض أنواع
+
+37
+00:02:35,240 --> 00:02:39,240
+ال media لما أنا أزرعها أزرع البكتيريا من ال bio
+
+38
+00:02:39,240 --> 00:02:42,680
+culture في هذه ال media بتكشفلي more specific
+
+39
+00:02:42,680 --> 00:02:46,750
+biochemical أكتر من فحص كيميائيهنتعرف في chapter
+
+40
+00:02:46,750 --> 00:02:51,830
+الـ Single Media Multiple Test على هذا الموضوع انه
+
+41
+00:02:51,830 --> 00:02:54,470
+ميديا انا بزرع البكتيريا في ميديا هذه الميديا
+
+42
+00:02:54,470 --> 00:03:00,050
+بتكشفلي على اكتر من فحص كيميائي كيف انا بدي استدل
+
+43
+00:03:00,050 --> 00:03:04,330
+انه التفاعل صار او لأ بقولنا من خلال نواتج التفاعل
+
+44
+00:03:04,330 --> 00:03:09,230
+بشوف النتائج ممكن بال next eye زي فحص الكتلاز كنت
+
+45
+00:03:09,230 --> 00:03:14,360
+اشوف ال air bubbles بعيني فحص ال coagulaseبرضه
+
+46
+00:03:14,360 --> 00:03:17,600
+ممكن اني انا اشوفها بالناقد I كنت اشوف الـ Clumpy
+
+47
+00:03:17,600 --> 00:03:24,660
+ممكن برضه اني انا اضيف Reagent في الميديا هذا الـ
+
+48
+00:03:24,660 --> 00:03:31,000
+Reagent يتفاعل مع نواتج التفاعل او يغير من اللغة
+
+49
+00:03:31,000 --> 00:03:34,960
+الميديا على سبيل المثال فحص السيطرات فحص السيطرات
+
+50
+00:03:34,960 --> 00:03:38,960
+كان في NDPH Indicator اللي هو الـ Bromothymol Blue
+
+51
+00:03:38,960 --> 00:03:45,560
+كان موجود من مكونات الميديالما التفاعل لو
+
+52
+00:03:45,560 --> 00:03:48,580
+البكتيريا عندها القدرة على استهلاك السيطرات قولنا
+
+53
+00:03:48,580 --> 00:03:51,860
+هيتكون الـ Climate Product اللي هو الـ Ammonia الـ
+
+54
+00:03:51,860 --> 00:03:56,660
+NH3 هيرفع الـ pH في عندي pH Indicator في الـ Media
+
+55
+00:03:56,660 --> 00:04:02,240
+هيتغير لون الـ Media حسب طبعا شو الـ pH Indicator
+
+56
+00:04:02,240 --> 00:04:06,220
+وشو لونه في ال Acidic أو الBasic حسب نواتج التفاعل
+
+57
+00:04:06,220 --> 00:04:11,060
+كان في السيطرات ناتج التفاعل Ammoniaالـ pH
+
+58
+00:04:11,060 --> 00:04:15,680
+Indicator في الـ Basic كان لونه blue يبقى هيتغير
+
+59
+00:04:15,680 --> 00:04:19,180
+لون ال media من اللون الأخضر لللون الأزرق هيك أنا
+
+60
+00:04:19,180 --> 00:04:24,200
+بعرف أنه البكتيريا قدرت تستهلك السيطرات يبقى هي
+
+61
+00:04:24,200 --> 00:04:30,180
+سيطرات بوزيفة هذا بالنسبة للـ Reagent أو الـ pH
+
+62
+00:04:30,180 --> 00:04:34,460
+Indicator كان موجود في ال media ممكن أنا بعد ال
+
+63
+00:04:34,460 --> 00:04:39,470
+incubationأضيف reagent يتفاعل مع نواتج التفاعل زي
+
+64
+00:04:39,470 --> 00:04:43,250
+فحص الـ amylase الـ amylase كنا نضيف ال iodine بعد
+
+65
+00:04:43,250 --> 00:04:47,250
+ال incubation يبقى نواتج التفاعل ممكن ان انا
+
+66
+00:04:47,250 --> 00:04:51,670
+اشوفها بالـ next I زي فحص الكتلاز والكواجولاز ممكن
+
+67
+00:04:51,670 --> 00:04:55,550
+ان انا اضيف pH indicator او reagent يتفاعل مع
+
+68
+00:04:55,550 --> 00:04:58,510
+نواتج التفاعل هذا reagent يكون موجود في ال media
+
+69
+00:04:58,510 --> 00:05:03,740
+زي فحص السيطرات او ممكن ان انا اضيف reagent بعدالـ
+
+70
+00:05:03,740 --> 00:05:08,300
+Incubation هذا زي فحص الـ amylase هذا الـ Reagent
+
+71
+00:05:08,300 --> 00:05:13,940
+هيتفاعل مع نواتج التفاعل لأنه ممكن يكون الـ
+
+72
+00:05:13,940 --> 00:05:18,280
+Reagent هذا بيتكسر في الـ Autoclave عشان هيك أنا
+
+73
+00:05:18,280 --> 00:05:21,760
+بضيفه بعد الـ Incubation الـ reaction البيو
+
+74
+00:05:21,760 --> 00:05:25,580
+كيميائية التي تأتي بـ Blast Candidatebe determined
+
+75
+00:05:25,580 --> 00:05:28,700
+by relatively simple indicator reagent بقول كيف
+
+76
+00:05:28,700 --> 00:05:32,440
+بستدل على التفاعل من خلال simple indicator reagent
+
+77
+00:05:32,440 --> 00:05:35,520
+هذا ال reagent ممكن يكون موجود في ال medium زي فحص
+
+78
+00:05:35,520 --> 00:05:39,380
+السيطرات أو ممكن أنا أضيفه على المزرعة بعد ال
+
+79
+00:05:39,380 --> 00:05:43,300
+incubation زي فحص ال amylase أو قولنا ممكن أني أنا
+
+80
+00:05:43,300 --> 00:05:48,560
+أشوف التفاعل بال next eye زي فحص الكتلاز و ال
+
+81
+00:05:48,560 --> 00:05:51,600
+coagulase some bacteria ferment carbohydrates
+
+82
+00:05:52,800 --> 00:05:57,520
+producing acidic alcohol or gases in products بقول
+
+83
+00:05:57,520 --> 00:06:00,540
+لي ان بعض البكتيريا عندها القدرة على تخمير
+
+84
+00:06:00,540 --> 00:06:05,880
+السكريات بتنتج acid or alcohol or gas يبقى نواتج
+
+85
+00:06:05,880 --> 00:06:10,880
+عملية ال fermentation acid or alcohol or gas بعض
+
+86
+00:06:10,880 --> 00:06:15,280
+أنواع الميكروبات بتنتج acid with gas وفي بعض الآخر
+
+87
+00:06:15,280 --> 00:06:21,510
+بتنتج ال acid without gasكيف بدنا نميز البكتيريا؟
+
+88
+00:06:21,510 --> 00:06:24,370
+many different species are distinguished on the
+
+89
+00:06:24,370 --> 00:06:28,250
+basis of the carbohydrate they do or don't attack
+
+90
+00:06:28,250 --> 00:06:31,970
+as well as by the nature of end product formed
+
+91
+00:06:31,970 --> 00:06:35,870
+during fermentation بقول لي بقدر أميز بين
+
+92
+00:06:35,870 --> 00:06:41,270
+الميكروبات من خلال أنواع الكربوهيدرات اللي بتستطيع
+
+93
+00:06:41,270 --> 00:06:47,360
+تخميرها يبقى بقدر أميز بين الميكروبات من خلالنوع
+
+94
+00:06:47,360 --> 00:06:52,940
+الكربوهيدرات اللى بتقدر انها اتخمرها كاف يعنى
+
+95
+00:06:52,940 --> 00:06:58,840
+قولنا انه اللى من انواع الكربوهيدرات الجليكوز
+
+96
+00:06:58,840 --> 00:07:02,280
+قولنا في بعض انواع البكتيريا بتقدر تخمر الجليكوز
+
+97
+00:07:02,280 --> 00:07:06,340
+جليكوز fermenter زى الانتيرو بكترياسي في بعض انواع
+
+98
+00:07:06,340 --> 00:07:09,720
+البكتيريا مابتقدر تخمر الجليكوز نون جليكوز
+
+99
+00:07:09,720 --> 00:07:17,070
+fermenter اللى هى الصدومانس من خلالهذا الفحص بقدر
+
+100
+00:07:17,070 --> 00:07:20,870
+ان انا اميز الصدومونس عن الانتيرو بكترياسي قولنا
+
+101
+00:07:20,870 --> 00:07:23,690
+ان الانتيرو بكترياسي بتنقسم ل lactose fermenter و
+
+102
+00:07:23,690 --> 00:07:28,630
+non lactose fermenter يبقى من خلال هذا الفحص بقدر
+
+103
+00:07:28,630 --> 00:07:32,650
+اميز عائلة الانتيرو بكترياسي او ان انا اقسمها
+
+104
+00:07:32,650 --> 00:07:37,280
+لمجموعات يساعدني في التعريفتاني إشي من خلال الـ
+
+105
+00:07:37,280 --> 00:07:40,620
+end product اللى اتكونت خلال عملية ال fermentation
+
+106
+00:07:40,620 --> 00:07:44,380
+قولنا نواتج عملية ال fermentation ممكن تكون acid
+
+107
+00:07:44,380 --> 00:07:49,080
+or alcohol or gas ممكن تكون acid لحاله ممكن تكون
+
+108
+00:07:49,080 --> 00:07:55,220
+acid with gas بكون أنا عارفة البكتيريا X بتعمل أو
+
+109
+00:07:55,220 --> 00:07:59,140
+نواتج عملية ال fermentation acid without gas
+
+110
+00:07:59,140 --> 00:08:04,140
+البكتيريا Y نواتج عملية ال fermentation acid with
+
+111
+00:08:04,140 --> 00:08:08,880
+gasيبقى من خلال نواتج الـ fermentation بقدر أني
+
+112
+00:08:08,880 --> 00:08:13,100
+أنا أتعرف على البكتيريا the nature of a product
+
+113
+00:08:13,100 --> 00:08:17,140
+formed in amino acid metabolism also provide
+
+114
+00:08:17,140 --> 00:08:20,940
+information as to the identification of bacterial
+
+115
+00:08:20,940 --> 00:08:24,500
+species بقولي برضه من خلال ال amino acid
+
+116
+00:08:24,500 --> 00:08:28,540
+metabolism ممكن أنها تقدملي معلومات تساعدني في
+
+117
+00:08:28,540 --> 00:08:33,020
+تعريف ال bacterial speciesعلى سبيل المثال في عندي
+
+118
+00:08:33,020 --> 00:08:37,300
+amino acid اسمه لتريبتوفان في بعض أنواع البكتيريا
+
+119
+00:08:37,300 --> 00:08:42,380
+عندها أنزيم لتريبتوفانياز بتكسر لتريبتوفان تمام
+
+120
+00:08:42,380 --> 00:08:47,560
+وبحصل على نوات اللي هو Indole, Pyrophate و أمونيا
+
+121
+00:08:47,560 --> 00:08:53,440
+الممكن إن أنا أستفيد طبعا من الأمونيا ك نيتروجين
+
+122
+00:08:53,440 --> 00:08:57,660
+سورس البيروفات فيه كمصدر للطاقة ك carbon source
+
+123
+00:08:57,660 --> 00:09:02,860
+هذا من خلال اللي هو in vivoIn vitro ممكن أستغل هذا
+
+124
+00:09:02,860 --> 00:09:07,260
+الانزيم عشان أعمله فحص كيميائي أشوف هل البكتيريا
+
+125
+00:09:07,260 --> 00:09:10,900
+عندها انزيم للـ tryptophanase أو لأ وهذا هيساعدني
+
+126
+00:09:10,900 --> 00:09:16,120
+في ال identification هنتعرف عليه بالتفصيل في ال
+
+127
+00:09:16,120 --> 00:09:20,700
+chapter اللي جاي في فحص الانضال test the
+
+128
+00:09:20,700 --> 00:09:23,740
+production of visible pigment distinguish certain
+
+129
+00:09:23,740 --> 00:09:28,700
+type of bacteria صدمونسي رشد ولبعض أنواع الصبغات
+
+130
+00:09:28,700 --> 00:09:34,740
+تستخدم للتمييزبين أنواع البكتيريا الصودموناس بتفرص
+
+131
+00:09:34,740 --> 00:09:39,440
+صبغة soluble pigment يبقى الصودموناس بتفرص صبغة
+
+132
+00:09:39,440 --> 00:09:52,260
+soluble pigment إيش
+
+133
+00:09:52,260 --> 00:09:57,180
+يعني soluble pigment هي لونها أصفر أو أخضرSoluble
+
+134
+00:09:57,180 --> 00:10:01,660
+pigment يعني بتلون الكلوني و كمان بتنتشر في الطبق
+
+135
+00:10:01,660 --> 00:10:05,800
+يبقى بلاقى الطبق كله الأجار مع الكلوني صار لونها
+
+136
+00:10:05,800 --> 00:10:10,220
+أخضر لكن السيراشيا بقولي insoluble pigment
+
+137
+00:10:30,530 --> 00:10:34,030
+السيراشيا in soluble pigment مايعني in soluble
+
+138
+00:10:34,030 --> 00:10:38,270
+pigment فقط هلاقي لون الكلوني ما هتقدر تنتشر في
+
+139
+00:10:38,270 --> 00:10:42,210
+الطبق لونها بيكون أحمر يبقى Sodomonas soluble
+
+140
+00:10:42,210 --> 00:10:46,310
+pigment مايعني soluble pigment يعني هتلون الكلوني
+
+141
+00:10:46,310 --> 00:10:50,810
+مع الطبق كامل باللون الأخضر أو الأصفر السيراشيا in
+
+142
+00:10:50,810 --> 00:10:55,710
+soluble pigment فقط هتلون الكلوني باللون الأحمرمش
+
+143
+00:10:55,710 --> 00:11:00,870
+الأجار لأنه هي insoluble ما هتقدر تدوب في المزرعة
+
+144
+00:11:00,870 --> 00:11:03,990
+working with a biological structure freshly
+
+145
+00:11:03,990 --> 00:11:08,190
+isolated from clinical specimen the microbiologist
+
+146
+00:11:08,190 --> 00:11:12,430
+uses a carefully selected petri of special media
+
+147
+00:11:12,430 --> 00:11:16,370
+to identify their outstanding biochemical
+
+148
+00:11:16,370 --> 00:11:21,280
+preparators بقوللي إنهخلال شغلي في الـ Biochemical
+
+149
+00:11:21,280 --> 00:11:24,340
+Test بأستخدم الـ Pure Culture وقلنا ليش Pure
+
+150
+00:11:24,340 --> 00:11:27,880
+Culture لأن أصلها خلية بكتيريا واحدة هيك بضمن أنه
+
+151
+00:11:27,880 --> 00:11:32,380
+أنا بعرف بكتيريا واحدة فقط اللي هي X الـ Fresh
+
+152
+00:11:32,380 --> 00:11:37,740
+لازم يكون لأن الـ Enzyme Activity بقل مع مرور
+
+153
+00:11:37,740 --> 00:11:42,180
+الوقت فبعطيني False Result الـ Microbiologist
+
+154
+00:11:42,180 --> 00:11:48,260
+بيقول لي بيختار ال media اللي هيزرع عليها العينات
+
+155
+00:11:48,970 --> 00:11:53,470
+عينايا على أي أساس احنا بنختار ال media بقولنا
+
+156
+00:11:53,470 --> 00:11:58,050
+بنختار ال media حسب نوع العينة ال stool لو وصلتني
+
+157
+00:11:58,050 --> 00:12:02,150
+عينة stool مش نفس ال media اللي هزرع عليها عينة ال
+
+158
+00:12:02,150 --> 00:12:06,270
+urine عشان هيك بدي أجيب media فقط بتنمي ال
+
+159
+00:12:06,270 --> 00:12:09,330
+pathogen ال pathogen الموجود في ال stool بيختلف عن
+
+160
+00:12:09,330 --> 00:12:12,670
+الموجود في ال urine يعني يكون ال common pathogen
+
+161
+00:12:12,670 --> 00:12:16,320
+in stoolيختلف عن الـ Common Pathogen in urine
+
+162
+00:12:16,320 --> 00:12:20,600
+فبالتالي أنا فقط بستخدم ميديا بتنمي الـ Pathogen
+
+163
+00:12:20,600 --> 00:12:27,340
+فبختار ميديا حسب نوع العينة هتتعرفوا في المعمل الـ
+
+164
+00:12:27,340 --> 00:12:31,960
+Diagnostic في الفصل الجاي كل عينة هيكون لها
+
+165
+00:12:31,960 --> 00:12:36,500
+chapter خاص كيف أنا أو شو الميديا اللي هزرع عليها
+
+166
+00:12:36,500 --> 00:12:37,720
+هذه العينة
+
+167
+00:12:46,580 --> 00:12:51,520
+Bacteria mainly produce ATP by بقولّي أن البكتيريا
+
+168
+00:12:51,520 --> 00:12:56,060
+بتنتج الـATP بتلت عملية أول عملية الـAerobic
+
+169
+00:12:56,060 --> 00:13:00,200
+Respiration تانية عملية الـfermentation تالت عملية
+
+170
+00:13:00,200 --> 00:13:03,560
+الـAnaerobic Respiration هنتعرف على كل عملية
+
+171
+00:13:03,560 --> 00:13:09,600
+بالتفصيل الـAerobic Respiration تنفس الهوائي is an
+
+172
+00:13:09,600 --> 00:13:13,500
+oxidative process which uses oxygen as final
+
+173
+00:13:13,500 --> 00:13:17,960
+electron acceptorبقول للتنفس الهوائي البكتيريا
+
+174
+00:13:17,960 --> 00:13:21,960
+اللى بتعمله لازم تكون obligate aerobic لازم تكون
+
+175
+00:13:21,960 --> 00:13:28,080
+هوائية إجبارية Oxidative process بتستخدم الأكسجين
+
+176
+00:13:28,080 --> 00:13:31,800
+ك final electron acceptor المستقبل النهائي
+
+177
+00:13:31,800 --> 00:13:35,340
+للإلكترونات بيكون الأكسجين الأكسجين يعتبر
+
+178
+00:13:35,340 --> 00:13:40,240
+inorganic molecule يبقىفي الـ Aerobic Respiration
+
+179
+00:13:40,240 --> 00:13:43,740
+بأستخدم الأكسجين in organic molecule للأكسجين
+
+180
+00:13:43,740 --> 00:13:47,680
+كمستقبل نهائي للإلكترونات خلال الـ Electron
+
+181
+00:13:47,680 --> 00:13:53,640
+Transport Chain قلنا بيصير اللي هو تفاعل أكسدة
+
+182
+00:13:53,640 --> 00:13:57,720
+واختزال المستقبل النهائي للإلكترونات الأكسجين
+
+183
+00:13:57,720 --> 00:14:01,080
+بيصير في عندي بيكون في عندي أنزيم الـ Oxidase الـ
+
+184
+00:14:01,080 --> 00:14:05,540
+Cytochrome C Oxidase بيعمل أكسدة للـ Cytochrome C
+
+185
+00:14:05,540 --> 00:14:09,950
+Reducedأكسى دا يعني فقد الإلكترونات هياخدوا
+
+186
+00:14:09,950 --> 00:14:13,510
+الإلكترون الـ Oxygen كمستقبل نهائي للإلكترونات
+
+187
+00:14:13,510 --> 00:14:18,970
+ويتكون الـH2O بالنهاية أنا بحصل من هذه العملية على
+
+188
+00:14:18,970 --> 00:14:26,370
+36 لـ 38 ATP pair جليكوز molecule كل جزء جليكوز
+
+189
+00:14:26,370 --> 00:14:34,710
+بينتج 36 ل 38 ATP ليش قالي 36 ل 38؟ 36 في حال كان
+
+190
+00:14:34,710 --> 00:14:40,930
+Eukaryoticالـ 38 في حال كان بره كديوتيك يبقى عملية
+
+191
+00:14:40,930 --> 00:14:45,310
+الـ Aerobic Respiration عملية بتتعامل مع ان الـ
+
+192
+00:14:45,310 --> 00:14:49,170
+Oxygen هو المستقبل النهائي للإلكترونات كمية الطاقة
+
+193
+00:14:49,170 --> 00:14:53,810
+اللي بتنتج من 36 لـ 38 بقوللي هي ال most efficient
+
+194
+00:14:53,810 --> 00:14:58,730
+form of the energy productionبالنسبة لتانية عملية
+
+195
+00:14:58,730 --> 00:15:02,730
+ال fermentation بستخدم organic molecule كمستقبل
+
+196
+00:15:02,730 --> 00:15:05,510
+نهائي للإلكترونات احنا قلنا في ال aerobic
+
+197
+00:15:05,510 --> 00:15:08,590
+respiration بستخدم inorganic molecule اللي هو
+
+198
+00:15:08,590 --> 00:15:12,310
+الأكسجين لكن في ال fermentation بستخدم organic
+
+199
+00:15:12,310 --> 00:15:16,510
+molecule كمستقبل نهائي للإلكترونات زي مين في عندي
+
+200
+00:15:16,510 --> 00:15:20,490
+organic molecule بقولي بشكل رئيسي الكربوهيدرات على
+
+201
+00:15:20,490 --> 00:15:25,100
+سبيل المثال جليكوز، لاكتاز، مالتاز، ساكرازالطاقة
+
+202
+00:15:25,100 --> 00:15:28,840
+اللي بتنتج خلال عملية ال fermentation بقول هي أقل
+
+203
+00:15:28,840 --> 00:15:33,820
+كفاءة من ال aerobic respiration فقط بيتم إنتاج 2
+
+204
+00:15:33,820 --> 00:15:39,780
+ATB بجليكوز molecule كل جليكوز molecule بينتج منه
+
+205
+00:15:39,780 --> 00:15:41,880
+فقط 2ATB
+
+206
+00:15:46,750 --> 00:15:50,170
+يبقى هذا بالنسبة لـ fermentation بأستخدم organic
+
+207
+00:15:50,170 --> 00:15:55,030
+molecule زي الكربوهيدرات كمية الطاقة التي تنتج to
+
+208
+00:15:55,030 --> 00:16:00,510
+ATP ال fermentation بتصير فقط في البكتيريا احنا
+
+209
+00:16:00,510 --> 00:16:03,750
+قولنا ال aerobic respiration فقط لازم تكون
+
+210
+00:16:03,750 --> 00:16:07,990
+البكتيريا هوائية إجبارية في ال fermentation بتكون
+
+211
+00:16:07,990 --> 00:16:12,170
+anaerobic بكتيريا بكتيريا لا هوائية وكمان
+
+212
+00:16:12,170 --> 00:16:17,600
+الفكولتات في anaerobicاللي هي الهوائية الاختيارية
+
+213
+00:16:17,600 --> 00:16:22,500
+ممكن انها تعمل fermentation and
+
+214
+00:16:22,500 --> 00:16:25,400
+aerobic respiration is similar to aerobic
+
+215
+00:16:25,400 --> 00:16:28,580
+respiration بقولي بتشبه ال aerobic respiration
+
+216
+00:16:28,580 --> 00:16:32,760
+لأنها بتستخدم inorganic molecule لكن في ال aerobic
+
+217
+00:16:32,760 --> 00:16:37,040
+respiration احنا قولنا بنستخدم inorganic molecule
+
+218
+00:16:37,040 --> 00:16:40,620
+اللي هو ال A2 لكن هنا انا بستخدم inorganic
+
+219
+00:16:40,620 --> 00:16:47,250
+molecule such as iron منجانيزقابلت نيترات صلفة
+
+220
+00:16:47,250 --> 00:16:51,430
+يبقى هاي اللي بستخدمها في الان أيوروبيك ريسبيراشن
+
+221
+00:16:51,430 --> 00:16:55,570
+ال inorganic molecule لكن في ال أيوروبيك بستخدم
+
+222
+00:16:55,570 --> 00:16:59,870
+الأكسجين كمستقبل نهائي للإلكترونات كمية الطاقة
+
+223
+00:16:59,870 --> 00:17:04,630
+اللي يتم إنتاجها لكل جزاء جيليوكوز بقول بتختلف حسب
+
+224
+00:17:04,630 --> 00:17:08,430
+نوع المستقبل النهائي للإلكترونات لو أنا استخدمت
+
+225
+00:17:08,430 --> 00:17:11,810
+ايرون كمية الطاقة بتختلف عن لو أنا استخدمت قابل
+
+226
+00:17:11,810 --> 00:17:18,080
+هذا بالنسبة لالان aerobic respiration كمية الطاقة
+
+227
+00:17:18,080 --> 00:17:22,580
+التي تنتج حسب المستقبل النهائي للإلكترونات يبقى ال
+
+228
+00:17:22,580 --> 00:17:25,280
+aerobic respiration بستخدم inorganic molecule
+
+229
+00:17:25,280 --> 00:17:30,440
+الأكسجين كمية الطاقة من 36 ل 38 ATP الآن aerobic
+
+230
+00:17:30,440 --> 00:17:34,660
+respiration بستخدم inorganic molecule زي ال iron
+
+231
+00:17:34,660 --> 00:17:38,360
+المنجنيز الكوبالت النيترات السلفات كمية الطاقة حسب
+
+232
+00:17:38,360 --> 00:17:42,350
+المستقبل النهائي للإلكتروناتالـ fermentation
+
+233
+00:17:42,350 --> 00:17:46,410
+باستخدم organic molecules زي الكربوهايدرات جمية
+
+234
+00:17:46,410 --> 00:17:51,810
+الطاقة فقط 2 ATP لكل جزاء جليكوز أخر جملة موجودة
+
+235
+00:17:51,810 --> 00:17:55,330
+في هاي ال slide بقول All fermentative bacteria can
+
+236
+00:17:55,330 --> 00:17:58,530
+ferment the simplest sugar اللي هو الديكستروز أو
+
+237
+00:17:58,530 --> 00:18:02,170
+الديجليكوز كل البكتيريا اللي انتها القدرة انها
+
+238
+00:18:02,170 --> 00:18:06,250
+تعمل ال fermentation بقول بتقدر تعمل fermentation
+
+239
+00:18:06,250 --> 00:18:10,740
+لأبسط سكر اللي هو الجليكوزالجليكوز قلنا
+
+240
+00:18:10,740 --> 00:18:15,580
+monosaccharide يبقى هو simple sugar بسيط فأي
+
+241
+00:18:15,580 --> 00:18:19,280
+بكتيريا بتقدر انها تعمل fermentation هتكون بتعمل
+
+242
+00:18:19,280 --> 00:18:23,180
+fermentation لأبسط ال sugar اللي هو الجليكوز
+
+243
+00:18:23,180 --> 00:18:27,800
+اللكتوز قلنا disaccharide اللي هو بتكون من two
+
+244
+00:18:27,800 --> 00:18:33,800
+sugar البكتيريا لو عملت تخمير للكتوز بتكون بالاصل
+
+245
+00:18:33,800 --> 00:18:38,900
+هي عاملة للجليكوز لأن اللكتوز يعتبر disaccharide
+
+246
+00:18:39,420 --> 00:18:43,100
+سكر معقد فأكيد بما أنها قدرت تعمل fermentation
+
+247
+00:18:43,100 --> 00:18:48,000
+للسكر المعقد هتكون عملت fermentation لل sugar
+
+248
+00:18:48,000 --> 00:18:52,640
+الأبسط منه اللي هو الجليكوز المونوسكري لكن العكس
+
+249
+00:18:52,640 --> 00:18:58,280
+مش صحيح يعني البكتيريا لو ماقدرت تعمل تخميد للكتاز
+
+250
+00:18:58,280 --> 00:19:01,700
+ممكن بالأصل تكون هي gelicose fermenter
+
+251
+00:19:06,660 --> 00:19:11,660
+النون لكتاز فيرمنتر هي مابتقدر تعمل تخميد للكتاز
+
+252
+00:19:11,660 --> 00:19:17,200
+لكن قدرت تعمل تخميد للجليكوز للسكر البسيطممكن انها
+
+253
+00:19:17,200 --> 00:19:20,660
+تكون في الاصل هي non-lactose fermenter وكمان non
+
+254
+00:19:20,660 --> 00:19:24,500
+-gelicose fermenter زي الصدومونس الصدومونس هي non
+
+255
+00:19:24,500 --> 00:19:28,080
+-gelicose fermenter فأكيد مش هتقدر تعمل تخمير
+
+256
+00:19:28,080 --> 00:19:32,320
+للسكر المعقد ماقدرت تعمل تخمير للسكر البسيط فاشي
+
+257
+00:19:32,320 --> 00:19:36,280
+طبيعي مش هتقدر تعمل تخمير للسكر المعقد اللاكتوز
+
+258
+00:19:36,280 --> 00:19:40,520
+يبقى بقول نهان انه اي بكتيريا بتعمل fermentation
+
+259
+00:19:40,520 --> 00:19:44,940
+هتكون بتقدر تعمل fermentation ل ال simplest sugar
+
+260
+00:19:44,940 --> 00:19:50,480
+اللي هو الجليكوزلو كانت عاملة تخمير لسكر معقد بدي
+
+261
+00:19:50,480 --> 00:19:56,680
+أفهم بشكل بديهي أنه هي قدرت تعمل تخمير للسكر
+
+262
+00:19:56,680 --> 00:20:02,320
+البسيط اللي هو الجليكوز لكن العكس مش صحيح ممكن
+
+263
+00:20:02,320 --> 00:20:07,000
+أنها تكون تقدر تعمل وممكن ما تكون تقدر تعمل
+
+264
+00:20:07,000 --> 00:20:12,320
+fermentation للجليكوز زي اللكتوزقلنا في الانتيرو
+
+265
+00:20:12,320 --> 00:20:16,100
+بكتيريا C في عندي نون لكتوز فرمنتر لكن هي في الأصل
+
+266
+00:20:16,100 --> 00:20:20,260
+قدرت تعمل للجليكوز في عندي نون لكتوز فرمنتر زي
+
+267
+00:20:20,260 --> 00:20:28,340
+السودومونس لكن هي بالأصل مابتقدر تعمل للجليكوز طيب
+
+268
+00:20:28,340 --> 00:20:31,220
+كيف بدي أنا أعرف هل البكتيريا عملت fermentation
+
+269
+00:20:31,220 --> 00:20:35,040
+ولا لأ هذا فحص اللي هو البكتيريا ال fermentation
+
+270
+00:20:35,040 --> 00:20:38,830
+كيف أنا بدي أعمل هذا الفحصالبرنسب اللي هذا الفحص
+
+271
+00:20:38,830 --> 00:20:41,690
+قولنا انه البكتيريا اللي بتقدر تعمل fermentation
+
+272
+00:20:41,690 --> 00:20:45,950
+أكيد هي تشتغل على ال substrate اللي هي
+
+273
+00:20:45,950 --> 00:20:50,070
+الكربوهايدرات هنحط طبعا جليكوز اللي هو أبسط sugar
+
+274
+00:20:50,070 --> 00:20:53,910
+في ال media كربوهايدرات نواتج عملية ال
+
+275
+00:20:53,910 --> 00:20:56,390
+fermentation أكيد ال fermentation هتشتغل على
+
+276
+00:20:56,390 --> 00:21:00,230
+الكربوهايدرات نواتج العملية acidic or alcohol
+
+277
+00:21:00,230 --> 00:21:04,370
+alcohol or gases and product نواتج العملية acid or
+
+278
+00:21:04,370 --> 00:21:07,980
+alcohol or gasكيف طريقة الشغل بدي احضر
+
+279
+00:21:07,980 --> 00:21:12,680
+fermentation tube بتكون هي ال broth مكونات هذه ال
+
+280
+00:21:12,680 --> 00:21:18,060
+tube ال nutrient ال broth ال nutrient ال broth اي
+
+281
+00:21:18,060 --> 00:21:21,100
+media سواء ال nutrient اجارة او ال nutrient ال
+
+282
+00:21:21,100 --> 00:21:25,200
+broth بتحتوي على مصادر للتغذية عشان تقدر تنمو
+
+283
+00:21:25,200 --> 00:21:28,960
+البكتيريا من مصادر التغذية هي في عندي بيبتون كمصدر
+
+284
+00:21:28,960 --> 00:21:33,770
+لل نيتروجين قلنا في عندي ممكن كمانBeef Extract
+
+285
+00:21:33,770 --> 00:21:40,290
+كمصادر لـ Carbon فين دي Phenol Red Phenol Red BH
+
+286
+00:21:40,290 --> 00:21:48,530
+Indicator بتركيز 0.00189% BH Indicator لأنه احنا
+
+287
+00:21:48,530 --> 00:21:52,450
+قلنا نواتج العملية acidic or alcohol فعشان اكشف عن
+
+288
+00:21:52,450 --> 00:21:57,060
+نواتج هايأو نواتج الدفاع اللى بستخدم BH Indicator
+
+289
+00:21:57,060 --> 00:22:00,420
+مين الباقي BH Indicator اللى بستخدمه اللى هو ال
+
+290
+00:22:00,420 --> 00:22:06,220
+Phenol Red بتركيز 0.00189
+
+291
+00:22:06,220 --> 00:22:10,440
+ال substrate اللى هيشتغل عليها الانزيم لازم تكون
+
+292
+00:22:10,440 --> 00:22:17,040
+في ال media اللى هى الجليكوز بنسبة 1.2% يبقى فيه
+
+293
+00:22:17,040 --> 00:22:20,720
+اندي أكيد هيكون جليكوز طيب احنا قلنا بدنا نكشف على
+
+294
+00:22:20,720 --> 00:22:25,510
+ال acid or الكحولمن خلال الفينار Red طب كيف بدي
+
+295
+00:22:25,510 --> 00:22:29,030
+أكشف على الجاز بقول في عندي درهام تيوب هي اللي
+
+296
+00:22:29,030 --> 00:22:32,750
+هتكشف ليه على ال gas product بتكون موجودة في
+
+297
+00:22:32,750 --> 00:22:38,890
+التيوب بتكون
+
+298
+00:22:38,890 --> 00:22:42,430
+موجودة بمقلوبة في التيوب بنحطها هنا
+
+299
+00:22:50,370 --> 00:22:55,450
+هذه الجرهام تيوب بحطها بشكل مقلوب هتقدر تكشفلي على
+
+300
+00:22:55,450 --> 00:23:00,730
+ال gas product طبعا انا بحطها قبل ما ادخلها اوتو
+
+301
+00:23:00,730 --> 00:23:05,970
+كلف لانه اذا كان موجود في عندي air bubbles في هاي
+
+302
+00:23:05,970 --> 00:23:09,270
+ال tube مقبل ال incubation مقبل ان انا ازرع ال
+
+303
+00:23:09,270 --> 00:23:13,130
+اوتو كلف هيتخلص من هاي ال air bubble عشان مايصير
+
+304
+00:23:13,130 --> 00:23:17,650
+في عندي اي false result لانهكيف النتيجة بتكون
+
+305
+00:23:17,650 --> 00:23:21,010
+بيكون عندي إذا كانت هي قدرت تنتج غاز بيكون في عندي
+
+306
+00:23:21,010 --> 00:23:25,450
+air bubbles في الـ tube عشان أمنع الـ false
+
+307
+00:23:25,450 --> 00:23:28,630
+positive result لازم أني أنا أدخلها الـ autoclave
+
+308
+00:23:28,630 --> 00:23:31,270
+بعدين أني أنا أزرع عشان إذا كان في عندي أي air
+
+309
+00:23:31,270 --> 00:23:36,090
+bubbles موجود ما قبل الinculation ال autoclave أنا
+
+310
+00:23:36,090 --> 00:23:40,690
+أتخلص منه من خلال الـ autoclave هي يبقى مكونات ال
+
+311
+00:23:40,690 --> 00:23:47,020
+media اللي فيه نوردشو الألوان تبعته بقول لي في non
+
+312
+00:23:47,020 --> 00:23:50,380
+-red في ال acidic بيكون لونه ياله في ال basic
+
+313
+00:23:50,380 --> 00:23:53,840
+بيكون لونه red في ال neutral بيكون لونه orange إذا
+
+314
+00:23:53,840 --> 00:23:59,740
+كان أقل من 86 بيكون acidic ما بين 86 و 84 بيكون ال
+
+315
+00:23:59,740 --> 00:24:04,600
+neutral إذا كان أعلى من 84 بيكون basic بيكون لونه
+
+316
+00:24:04,600 --> 00:24:09,960
+أحمرهعمل انكيولات لأ البكتيريا اللى انا بدي افحص
+
+317
+00:24:09,960 --> 00:24:15,260
+هل بتقدر تعمل fermentation ولا لأ هايبروث هاخد ال
+
+318
+00:24:15,260 --> 00:24:20,800
+ال ال انيكولام و هعمل mix في هاي ليتيوب هعمل
+
+319
+00:24:20,800 --> 00:24:27,300
+incubation لمدة 24 ساعة على 37 درجة سيليسيا بعد 24
+
+320
+00:24:27,300 --> 00:24:32,200
+ساعة بقولي النتائج بتكون كالتالي اما هتتغير لون
+
+321
+00:24:32,200 --> 00:24:37,110
+ليتيوب لللون ال yellow اش يعنيبقولي هذا معناه ان
+
+322
+00:24:37,110 --> 00:24:41,570
+البكتيريا قدرت تعمل fermentation للجليكوز نواتج
+
+323
+00:24:41,570 --> 00:24:45,730
+عملية ال fermentation أسدك اتكون أسد فبالتالي
+
+324
+00:24:45,730 --> 00:24:49,470
+هيعمل على خفض ال بي اتش وهيتحول اللون للون ييلو
+
+325
+00:24:49,470 --> 00:24:53,090
+ييلو زائد air bubble ال air bubble هتكون في
+
+326
+00:24:53,090 --> 00:24:55,690
+الدرهمت يوب بقولي ان البكتيريا قدرت تعمل
+
+327
+00:24:55,690 --> 00:24:59,330
+fermentation للجليكوز نواتج عملية ال fermentation
+
+328
+00:24:59,330 --> 00:25:04,350
+أسد قللت ال بي اتش اتحول اللون للون أصفروبنفس
+
+329
+00:25:04,350 --> 00:25:07,210
+الوقت كمان كان من نواتج عملية ال fermentation
+
+330
+00:25:07,210 --> 00:25:11,850
+أنتجت gas كيف بدي أكشف عنه من خلال الدرهام tube
+
+331
+00:25:11,850 --> 00:25:15,170
+هشوف air bubbles في الدرهام tube يبقى هاي النتيجة
+
+332
+00:25:15,170 --> 00:25:19,470
+بتكون نواتج التفاعل أو نواتج ال fermentation يكون
+
+333
+00:25:19,470 --> 00:25:25,850
+acid with gas لكن هاي acid without gas Orange زائد
+
+334
+00:25:25,850 --> 00:25:30,030
+air bubblesأورنج هلأ عملت fermentation ولا لأ لأ
+
+335
+00:25:30,030 --> 00:25:33,130
+لأن الأورنج بيكون feeling neutral ماتكون عندي
+
+336
+00:25:33,130 --> 00:25:38,370
+product acidic طيب كيف نتج الغاز عندي و هو من
+
+337
+00:25:38,370 --> 00:25:42,110
+نواتج عملية ال fermentation بيقول لي هان بيكون مش
+
+338
+00:25:42,110 --> 00:25:46,250
+مسكرين الأنبوبة امنيح بيكون الغاز من برة مصر في
+
+339
+00:25:46,250 --> 00:25:50,130
+عندي fermentation لكن ال air bubbles اتكون لنتيجة
+
+340
+00:25:50,130 --> 00:25:56,070
+ان ال tube كانت مش مسكرة امنيح ال red اللون الأحمر
+
+341
+00:25:56,300 --> 00:25:58,920
+أكيد مش هيكون في عندي fermentation لأن اللون
+
+342
+00:25:58,920 --> 00:26:05,560
+الأحمر موجود في ال basic في حال كان في عندي
+
+343
+00:26:05,560 --> 00:26:10,360
+alkaline ما أصبح في عندي fermentation طيب شو سبب
+
+344
+00:26:10,360 --> 00:26:14,560
+اللون الأحمر بقول لي أنه يكون نتيجة ال deamination
+
+345
+00:26:14,560 --> 00:26:18,140
+of بيبتون من مكونات ال media في عندي بيبتون
+
+346
+00:26:18,140 --> 00:26:22,220
+البيبتون هذاالبكتيريا اللى ماقدرت تعمل
+
+347
+00:26:22,220 --> 00:26:25,880
+fermentation للجليكوز هتروح لمصادر تانية هتروح
+
+348
+00:26:25,880 --> 00:26:31,520
+للبيبتون تعمل deamination هتنزع الـ N-H3
+
+349
+00:26:31,520 --> 00:26:36,160
+Deamination ايش معناها انها تنزع الامانيا البيبتون
+
+350
+00:26:36,160 --> 00:26:41,140
+اللى هو ال amino acid ال amino acid احد اللى هو
+
+351
+00:26:41,140 --> 00:26:46,380
+مكوناته او ال structure سبعته بيكون فيه اندى N-H3
+
+352
+00:26:47,020 --> 00:26:50,260
+البكتيريا هتعمل الـ Deamination هتنزع الـ NH3
+
+353
+00:26:50,260 --> 00:26:54,300
+الأمونيا عشان تحصل على نيتروجين سارس تقدر تنمو
+
+354
+00:26:54,300 --> 00:26:58,820
+وتتكثر اتكون الـ NH3 أمونيا الأمونيا الالكالاين
+
+355
+00:26:58,820 --> 00:27:05,080
+هيرفع الـ pH وتتحول لون ال ميديا للون أحمر هيك
+
+356
+00:27:05,080 --> 00:27:08,680
+بنكون خلصنا ال bacterial fermentation قولنا ال
+
+357
+00:27:08,680 --> 00:27:11,480
+bacterial fermentation عشان ال principle انه
+
+358
+00:27:11,480 --> 00:27:15,520
+carbohydrate الشيليوكاز بيكون موجود في الميديالو
+
+359
+00:27:15,520 --> 00:27:18,300
+البكتيريا قدرت تعمل fermentation النواتج هتكون
+
+360
+00:27:18,300 --> 00:27:22,700
+acid or alcohol or gas كيف بدي أكشف عن ال acid؟ في
+
+361
+00:27:22,700 --> 00:27:26,160
+عندي pH indicator اللي هو الفينار Red كيف بدي أكشف
+
+362
+00:27:26,160 --> 00:27:29,640
+على ال gas؟ في عندي درهام تيوب بقدر أكشف على ال
+
+363
+00:27:29,640 --> 00:27:36,020
+gas عرفنا كل launch و تفسير هذه الألوان بالنسبة
+
+364
+00:27:36,020 --> 00:27:39,600
+للـ Significant لهذا الفحص الـ Significant لهذا
+
+365
+00:27:39,600 --> 00:27:43,660
+الفحص اللي هو هيميز بين اللي قلناه ال gelicose
+
+366
+00:27:43,660 --> 00:27:48,090
+fermenterبين positive و negative بين الصدومونس وال
+
+367
+00:27:48,090 --> 00:27:53,910
+انتيرو بكترياسي كمان في اندي في ال انتيرو بكترياسي
+
+368
+00:27:53,910 --> 00:27:56,690
+ممكن انها تميز ال lactose fermenter عن ال non
+
+369
+00:27:56,690 --> 00:27:59,210
+lactose fermenter هذا بالنسبة ل significant
+
+370
+00:28:08,880 --> 00:28:11,960
+Fermentation of Carbohydrate Carbohydrate are
+
+371
+00:28:11,960 --> 00:28:15,680
+complex chemical substrate which serves as energy
+
+372
+00:28:15,680 --> 00:28:19,880
+source قولنا إن الـ Carbohydrate هي معقدة بتكون
+
+373
+00:28:19,880 --> 00:28:24,380
+مصدر للطاقة و كمان لـ Carbon Source لما تتكسر
+
+374
+00:28:24,380 --> 00:28:28,880
+بواسطة البكتيريا and other cells أكيد أنا لما
+
+375
+00:28:28,880 --> 00:28:32,640
+تتكسر بحصل عليه من خلالها علاقة طاقة و بحصل على
+
+376
+00:28:32,640 --> 00:28:35,640
+Carbon Source هذا الـ Carbon Source مهم لنموء
+
+377
+00:28:35,640 --> 00:28:40,530
+الخلية الخلية البكتيريةمكونات الـ Carbohydrate,
+
+378
+00:28:40,630 --> 00:28:46,170
+Carbon, Hydrogen and Oxygen حسب هذه العلاقة ممكن
+
+379
+00:28:46,170 --> 00:28:49,810
+أنه يكون صنفه إلى Sugar or Starch الـ Sugar اللي
+
+380
+00:28:49,810 --> 00:28:52,970
+هي الـ Monodiosaccharide، الـ Starch قولنا الـ
+
+381
+00:28:52,970 --> 00:28:54,210
+Polysaccharide
+
+382
+00:28:56,080 --> 00:29:00,240
+facultative anaerobic and anaerobic bacteria are
+
+383
+00:29:00,240 --> 00:29:02,540
+capable of fermentation قولت أنا عملية ال
+
+384
+00:29:02,540 --> 00:29:05,560
+fermentation بتقدر اللي هي ال facultative
+
+385
+00:29:05,560 --> 00:29:09,620
+anaerobic and anaerobic bacteria قولنا ال
+
+386
+00:29:09,620 --> 00:29:13,420
+anaerobic respiration فقط ال obligate anaerobic هي
+
+387
+00:29:13,420 --> 00:29:17,060
+اللي بتقدر تعمل ال anaerobic respiration لكن ال
+
+388
+00:29:17,060 --> 00:29:20,780
+fermentation ال anaerobic bacteria و ال
+
+389
+00:29:20,780 --> 00:29:23,560
+facultative anaerobic bacteria بتقدر تعمل
+
+390
+00:29:23,560 --> 00:29:28,050
+fermentationالفكولتات الـ anaerobic هي الهوائية
+
+391
+00:29:28,050 --> 00:29:32,390
+الإختيارية لأن الـ fermentation يقولنا بيحصل في
+
+392
+00:29:32,390 --> 00:29:35,870
+ظروف لا هوائية عشان هيك أشي طبيعي الـ anaerobic
+
+393
+00:29:35,870 --> 00:29:38,830
+بكتيريا تقدر تعمل الـ fermentation الفكولتات الـ
+
+394
+00:29:38,830 --> 00:29:43,090
+anaerobic في حال ما عندي أكسجين أكيد بتستطيع العيش
+
+395
+00:29:43,090 --> 00:29:48,290
+بدون أكسجين فبالتالي بتقدر تعمل fermentationلكن
+
+396
+00:29:48,290 --> 00:29:52,310
+الـ Obligate Aerobic Bacteria ما بتقدر تعمل
+
+397
+00:29:52,310 --> 00:29:56,030
+fermentation ل الجليكوز ده هي الصدومونس الصدومونس
+
+398
+00:29:56,030 --> 00:30:00,490
+تعتبر Obligate Aerobic تمام قلنا هي Non-Gelicose
+
+399
+00:30:00,490 --> 00:30:04,370
+Fermenter شو تعريف عملية ال fermentation هاني معرف
+
+400
+00:30:04,370 --> 00:30:09,530
+عملية ال fermentation Un-Aerobic Process هي عملية
+
+401
+00:30:09,530 --> 00:30:12,190
+لا هوائية عشان هي فقط اللي بتقدر تعمل ال
+
+402
+00:30:12,190 --> 00:30:15,210
+fermentation ال Un-Aerobic Bacteria والفكولتات في
+
+403
+00:30:15,210 --> 00:30:18,710
+Un-Aerobicما بتقدر تعمل الـ obligate aerobic زي
+
+404
+00:30:18,710 --> 00:30:22,690
+الـ Sodomonas during which carbohydrates are
+
+405
+00:30:22,690 --> 00:30:26,690
+broken down for energy production بيقوللي بيتم
+
+406
+00:30:26,690 --> 00:30:36,390
+تكسير ال carbohydrates عشان أحصل على الطاقة a
+
+407
+00:30:36,390 --> 00:30:40,370
+wide variety of carbohydrates may be fermented by
+
+408
+00:30:40,370 --> 00:30:44,240
+various bacteria in order to obtain energyبقولي
+
+409
+00:30:44,240 --> 00:30:50,440
+كتير من ال carbohydrate بيتم تخميرها بواسطة أنواع
+
+410
+00:30:50,440 --> 00:30:54,800
+مختلفة من البكتيريا عشان أحصل على الطاقة and the
+
+411
+00:30:54,800 --> 00:30:57,240
+types of carbohydrate بقولي الأنواع ال
+
+412
+00:30:57,240 --> 00:31:00,420
+carbohydrate اللي بيتم تخميرها by specific
+
+413
+00:31:00,420 --> 00:31:04,320
+organism can service as a diagnostic tool for the
+
+414
+00:31:04,320 --> 00:31:07,840
+identification of that organism قولناحسب
+
+415
+00:31:07,840 --> 00:31:11,040
+الكربوهايدرات اللى بيتم تخميرها وحسب الـ end
+
+416
+00:31:11,040 --> 00:31:14,160
+product أنا هدولا للاتنين بقدر استخدمهم ك
+
+417
+00:31:14,160 --> 00:31:18,400
+diagnostic tool عشان أقدر أعرف البكتيريا ممكن ال
+
+418
+00:31:18,400 --> 00:31:21,920
+ال interior bacteriasi صنفناها لـ lactose
+
+419
+00:31:21,920 --> 00:31:25,000
+fermenter و non lactose fermenter من خلال هذا
+
+420
+00:31:25,000 --> 00:31:28,800
+الفحص بقدر نقسم ال interior bacteriasi لمجموعات
+
+421
+00:31:28,800 --> 00:31:33,190
+وهذا هيساعدني في ال identificationكمان نواتش عملية
+
+422
+00:31:33,190 --> 00:31:37,310
+ال fermentation قلنا ممكن يكون acid or acid with
+
+423
+00:31:37,310 --> 00:31:40,110
+gas فبالتالي برضه هذا هيساعدني في ال
+
+424
+00:31:40,110 --> 00:31:46,530
+identification للorganism نواتش عملية ال
+
+425
+00:31:46,530 --> 00:31:49,710
+fermentation ممكن يكون acid and product وممكن يكون
+
+426
+00:31:49,710 --> 00:31:54,860
+acid with gas and productعشان اكشف على الـ acid
+
+427
+00:31:54,860 --> 00:31:58,540
+production بقول من خلال الـ pH Indicator اللي هو
+
+428
+00:31:58,540 --> 00:32:02,580
+الفينول Red نحفظ حدود الفينول Red في ال acidic
+
+429
+00:32:02,580 --> 00:32:06,040
+بيكون لونه Yellow في ال basic بيكون لونه Red
+
+430
+00:32:06,040 --> 00:32:10,060
+بيتحول إذا كان في عندي acidic product بتتحول لون
+
+431
+00:32:10,060 --> 00:32:15,360
+ال media للون Yellow إذا كان ال ال pH أقل من 6 و 8
+
+432
+00:32:15,360 --> 00:32:19,820
+عشان أكشف على الجاز بحط درهم إتيوب هاي الدرهم
+
+433
+00:32:19,820 --> 00:32:23,400
+إتيوب في عندي اللي هي إتيوب مقلوبةإذا شوفت في عندي
+
+434
+00:32:23,400 --> 00:32:27,600
+air bubbles يبقى هان في عندي gas كان من نواتج
+
+435
+00:32:27,600 --> 00:32:31,800
+عملية ال fermentation بكتيريا produce acidic
+
+436
+00:32:31,800 --> 00:32:34,620
+product when they ferment certain carbohydrate
+
+437
+00:32:34,620 --> 00:32:39,720
+بقوللي أن البكتيريا بتنتج acid product لما اتخمر
+
+438
+00:32:39,720 --> 00:32:42,920
+أنواع معينة من ال carbohydrate ال carbohydrate
+
+439
+00:32:42,920 --> 00:32:46,740
+utilization test فحص ال carbohydrate utilization
+
+440
+00:32:46,740 --> 00:32:49,500
+اللي أنا شرحته قبل شوية
+
+441
+00:32:57,570 --> 00:33:01,090
+مصمم من خلال اني انا اكشف على التغير في ال بي اتش
+
+442
+00:33:01,090 --> 00:33:05,350
+اذا صار في عندي fermentation ولا لأ التغير في ال
+
+443
+00:33:05,350 --> 00:33:09,790
+بي اتش لانه ال fermentation نواتج ال fermentation
+
+444
+00:33:09,790 --> 00:33:14,370
+acid فبالتالي اذا تغير ال بي اتش يبقى صار في عندي
+
+445
+00:33:14,370 --> 00:33:18,880
+fermentationacid lowers the pH of the media بقوللي
+
+446
+00:33:18,880 --> 00:33:22,160
+ان ال acid بيعمل لخفض ال pH في ال media فبالتالي
+
+447
+00:33:22,160 --> 00:33:26,720
+بيخلي ال pH indicator يتحول لللون ال yellow لو
+
+448
+00:33:26,720 --> 00:33:30,280
+البكتيريا ماقدرت اتخمر ال carbohydrate بقوللي ان
+
+449
+00:33:30,280 --> 00:33:34,300
+ال media هيظل لونها أحمر او هتعمل deamination ل
+
+450
+00:33:34,300 --> 00:33:39,020
+الببطان هتنزع ال ammonia ال NH3 ال NH3 alkaline
+
+451
+00:33:39,020 --> 00:33:43,920
+فبالتالي هيتحول لون ال media لللون الأحمر if gas
+
+452
+00:33:43,920 --> 00:33:48,700
+is produced as byby product of fermentation then
+
+453
+00:33:48,700 --> 00:33:52,040
+the Durham tube will have a bubble in it بقولي لو
+
+454
+00:33:52,040 --> 00:33:56,860
+اتكون gas من ال fermentation انا بقدر اكشف عليه من
+
+455
+00:33:56,860 --> 00:34:03,000
+خلال اني انا بشوف air bubbles في ال Durham tube ال
+
+456
+00:34:03,000 --> 00:34:05,800
+carbohydrate اللي ممكن اني انا اكشف عنها جليكوز
+
+457
+00:34:05,800 --> 00:34:09,260
+لاكتوز ساكروس بقولي all carbohydrate test media
+
+458
+00:34:09,260 --> 00:34:13,160
+should be inoculated with a transfer loop عشان
+
+459
+00:34:13,160 --> 00:34:16,700
+اعمل الفحص بستخدم اللي هو ال transfer tubeهذا
+
+460
+00:34:16,700 --> 00:34:21,700
+بالنسبة للـ result لـ fermentation ملاحظ في tube 1
+
+461
+00:34:21,700 --> 00:34:26,660
+و tube 3 تكون اللون ال yellow يبقى فى صار فى عندي
+
+462
+00:34:26,660 --> 00:34:32,080
+fermentation لجليكوز اتكون أسد اتحول اللون للون
+
+463
+00:34:32,080 --> 00:34:37,040
+أصفر طبعا نتيجة اللى هو ال decrease ل ال pH ملاحظ
+
+464
+00:34:37,040 --> 00:34:42,380
+انه high لونها أخف من high هنتعرف كمان شوية ان في
+
+465
+00:34:42,380 --> 00:34:47,110
+بعض أنواع البكتيريا بتعمل fermentation لالجليكوز
+
+466
+00:34:47,110 --> 00:34:51,770
+وبتنتج كمية كبيرة من ال acid أو بتنتج أحماض بتعمل
+
+467
+00:34:51,770 --> 00:34:57,810
+على خفض البيئة مش بصورة كبيرة فبالتالي بيكون اللون
+
+468
+00:34:57,810 --> 00:35:02,170
+غامق كتير لكن ها نتكون acid لكن مش بصورة كبيرة في
+
+469
+00:35:02,170 --> 00:35:06,790
+عندي air bubbles هاي ال tube اللي بالنص تكون اللون
+
+470
+00:35:06,790 --> 00:35:09,570
+ال red يبقى ما صار في عندي fermentation صار في
+
+471
+00:35:09,570 --> 00:35:15,620
+عندي deamination لأ النجاتيف لفرمنتشن تيوب وان و
+
+472
+00:35:15,620 --> 00:35:24,040
+ثري بوزيتيف لفرمنتشن طيب
+
+473
+00:35:24,040 --> 00:35:30,020
+هلأ لو عملت إجرام استان و طلعت إجرام نيجاتيف بصلة
+
+474
+00:35:30,020 --> 00:35:32,920
+ماهو الخطوة التانية الخطوة التانية أني أنا بدي
+
+475
+00:35:32,920 --> 00:35:36,400
+أعمل فحص ال Oxidase قلنا في المعمل السابق عن هذا
+
+476
+00:35:36,400 --> 00:35:39,490
+الموضوعإذا طلعت positive يبقى فيها عندى احتمال
+
+477
+00:35:39,490 --> 00:35:42,870
+Sodamonase, Vibrio, Campylobacter, Helicobacter,
+
+478
+00:35:43,310 --> 00:35:46,450
+Irimonase قولنا هي Oxidase positive لو كانت
+
+479
+00:35:46,450 --> 00:35:49,370
+negative فيها عندى احتمال Enterobacteraceae و
+
+480
+00:35:49,370 --> 00:35:56,310
+Acinetobacter طيب عشان أفرق بين هدولة ال positive,
+
+481
+00:35:56,550 --> 00:35:58,930
+oxidase positive, oxidase negative إذا طلعت
+
+482
+00:35:58,930 --> 00:36:00,630
+Oxidase positive
+
+483
+00:36:03,420 --> 00:36:06,240
+يبقى عندي احتمال قولنا الـ Vibrio, Campylobacter,
+
+484
+00:36:06,460 --> 00:36:09,260
+Helicobacter, Aeromonas و Sodomonas لو طلعت أكسداز
+
+485
+00:36:09,260 --> 00:36:13,380
+negative انتيروبكترياسي أسينيتوباكتر عشان أفرق ال
+
+486
+00:36:13,380 --> 00:36:16,960
+positive بقولي بتعملي فحص الجليكوز fermentation لو
+
+487
+00:36:16,960 --> 00:36:20,200
+طلعت negative قولنا Sodomonas obligate aerobic
+
+488
+00:36:20,200 --> 00:36:25,840
+فبالتالي أكيد هتكون non-gelicose fermenter لو طلعت
+
+489
+00:36:25,840 --> 00:36:29,040
+positive يبقى في عندي أربع احتمالات Vibrio,
+
+490
+00:36:29,160 --> 00:36:33,060
+Campylobacter, Helicobacter, Aeromonasبالنسبة لأ
+
+491
+00:36:33,060 --> 00:36:36,160
+الأكسداز negative عشان أفرق الـ Enterobacteriase و
+
+492
+00:36:36,160 --> 00:36:38,660
+الـ Acinetobacter برضه بعمل فحص الجليكوز
+
+493
+00:36:38,660 --> 00:36:41,320
+fermentation لو طلعت positive يبقى هي
+
+494
+00:36:41,320 --> 00:36:43,740
+Enterobacteriase الـ Enterobacteriase تعتبر
+
+495
+00:36:43,740 --> 00:36:47,600
+facultative and aerobic كلنا facultative and
+
+496
+00:36:47,600 --> 00:36:51,060
+aerobic بكتيريا بتقدر تعمل fermentation لو طلعت
+
+497
+00:36:51,060 --> 00:36:55,160
+negative يبقى هي Acinetobacterبرضه ال انتيرو
+
+498
+00:36:55,160 --> 00:36:58,460
+بكترياسي قلنا بنصنفها لمجموعتان اللي هي lactose
+
+499
+00:36:58,460 --> 00:37:01,780
+fermenter و non lactose fermenter ممكن ان انا اعمل
+
+500
+00:37:01,780 --> 00:37:06,020
+هذا الفحص عشان اقدر ان انا اسم ال انتيرو بكترياسي
+
+501
+00:37:06,020 --> 00:37:10,420
+لمجموعات وهذا هيساعدني في التعريف كيف فحص ال
+
+502
+00:37:10,420 --> 00:37:14,680
+lactose fermenter هنتعرف عليه في ال differential
+
+503
+00:37:14,680 --> 00:37:19,140
+media من خلال المقنكي المقنكي هي تعتبر
+
+504
+00:37:19,140 --> 00:37:22,440
+differential media هتقدر تتميز اللي lactose و non
+
+505
+00:37:22,440 --> 00:37:26,870
+lactose fermenterيبقى هي حسب النوع الـ
+
+506
+00:37:26,870 --> 00:37:30,010
+carbohydrate أنا الـ type of carbohydrate اقدرت ان
+
+507
+00:37:30,010 --> 00:37:34,970
+انا اعمل identification للبكتيريا هيك بنكون خلصنا
+
+508
+00:37:34,970 --> 00:37:38,770
+اول فحص اللي هو ال bacterial fermentation هلأ
+
+509
+00:37:38,770 --> 00:37:42,810
+هنتعرف على فحص ال methyl red vucus بسكوار وهو
+
+510
+00:37:42,810 --> 00:37:44,850
+بيتعلق برضه في ال fermentation
+
diff --git a/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/zA7UNireE5w_raw.srt b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/zA7UNireE5w_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..0dea63cf7d24086a7c1370abe01605ca4fd5caae
--- /dev/null
+++ b/PL9fwy3NUQKwZWgAb_V1ATlRRsH-6i3cPC/zA7UNireE5w_raw.srt
@@ -0,0 +1,1348 @@
+1
+00:00:00,520 --> 00:00:04,220
+السلام عليكم ورحمة الله وبركاته يعطيكم العافية
+
+2
+00:00:04,220 --> 00:00:08,160
+جميعا نستكمل معاكم محاضرات مساق الـ Microbiology
+
+3
+00:00:08,160 --> 00:00:12,060
+العملي في المحاضرة السابقة اتعرفنا على الستان
+
+4
+00:00:12,060 --> 00:00:15,700
+قسمنا الصبغات حسب ال function ل simple stand،
+
+5
+00:00:15,700 --> 00:00:18,840
+differential stand و special stand في هذا اللقاء
+
+6
+00:00:18,840 --> 00:00:21,720
+هنتناول أخر نوع من أنواع الصبغات اللي هو ال
+
+7
+00:00:21,720 --> 00:00:26,010
+special standالـ specialists قلنا تستخدم to view
+
+8
+00:00:26,010 --> 00:00:29,770
+specific part of bacteria specific part يعني
+
+9
+00:00:29,770 --> 00:00:35,050
+special component هذه المكونات عدم وجودها لا يؤثر
+
+10
+00:00:35,050 --> 00:00:39,000
+على الخلية البكتيريةفي المقابل في عندي essential
+
+11
+00:00:39,000 --> 00:00:44,060
+component هي مكونات عمق دم وجودها هيأثر على الخلية
+
+12
+00:00:44,060 --> 00:00:49,480
+البكتيرية زي مين essential component سلمبران سلوال
+
+13
+00:00:49,480 --> 00:00:53,780
+الـcytoplasm النيوكلياس كلها هي تعتبر essential
+
+14
+00:00:53,780 --> 00:00:58,460
+component الخلية البكتيرية ما بتقدر تستغني عنها
+
+15
+00:00:58,460 --> 00:01:03,600
+لكن بالنسبة لال special component من الأمثلة عندي
+
+16
+00:01:03,600 --> 00:01:08,970
+الـSpar الكابسول الفلاجلةهنتعرف في هذه المحاضرة
+
+17
+00:01:08,970 --> 00:01:13,090
+كيف بدي أنا أصبغ ال special component عندي ال
+
+18
+00:01:13,090 --> 00:01:16,470
+Indospore stain، ال capsule stain و ال flagella
+
+19
+00:01:16,470 --> 00:01:20,870
+stain أول special component هنتكلم عنه اللي هو ال
+
+20
+00:01:20,870 --> 00:01:26,510
+spar Indospore staining صباغة ال Indospore the
+
+21
+00:01:26,510 --> 00:01:30,270
+name Indospore is suggestive of a spar or seed
+
+22
+00:01:30,270 --> 00:01:35,440
+like form بقوللي أنه شكل البوغ بيشبه البذورفي جملة
+
+23
+00:01:35,440 --> 00:01:39,640
+اكتير سمعناها ان الأبواق في البكتيريا تستخدم
+
+24
+00:01:39,640 --> 00:01:43,800
+للحماية for protection لكن في الفطريات تستخدم
+
+25
+00:01:43,800 --> 00:01:50,120
+للتكاثر كالبذور في النباتات Indo means within
+
+26
+00:01:50,120 --> 00:01:54,560
+بقولنا طول ما الاسبار داخل الخلية بسميه Indo-espar
+
+27
+00:01:54,560 --> 00:01:59,360
+بعد كده ممكن تبدأ تتحلل البكتيريا ويطلع الاسبار
+
+28
+00:01:59,360 --> 00:02:04,460
+واسميه Re-espar لكن طول ما هو داخل الخلية بسميه
+
+29
+00:02:04,720 --> 00:02:09,140
+Indospore بقولنا ان البكتيريا لما تكون في حالتها
+
+30
+00:02:09,140 --> 00:02:13,740
+الطبيعية و تكون الظروف مهيئة لانه مو التكاثر بسمي
+
+31
+00:02:13,740 --> 00:02:18,280
+الخلية vegetative cell خلية تكاثرية هي ال
+
+32
+00:02:18,280 --> 00:02:22,440
+vegetative cell في حال اتغيرت الظروف و أصبحت غير
+
+33
+00:02:22,440 --> 00:02:28,300
+مناسبة لانه مو البكتيريا بتقوم بتكوين الأبواغ ال
+
+34
+00:02:28,300 --> 00:02:32,460
+sport كنوع من أنواع الحماية من العوامل الخارجية في
+
+35
+00:02:32,460 --> 00:02:37,520
+هذه الحالةلما تتحول ال vegetative cell ل spar بسمي
+
+36
+00:02:37,520 --> 00:02:42,580
+العملية sparulation لكن لو اتحسنت الظروف و تم
+
+37
+00:02:42,580 --> 00:02:47,600
+تزويدها بمغضيات راح ترجع ال spar ل vegetative cell
+
+38
+00:02:47,600 --> 00:02:52,790
+بعملية راح ترجع ال sparلـ vegetative cell بعملية
+
+39
+00:02:52,790 --> 00:02:57,050
+اسمها ال germination يبقى ال speculation هي عملية
+
+40
+00:02:57,050 --> 00:03:00,270
+تحويل ال vegetative cell ل endospar لو اتحسنت
+
+41
+00:03:00,270 --> 00:03:03,530
+الظروف هترجع ال endospar ل vegetative cell في
+
+42
+00:03:03,530 --> 00:03:09,350
+عملية ال germination ال endospar يعتبر resting
+
+43
+00:03:09,350 --> 00:03:15,600
+structure يعني resting يعني خامل يعني non activeby
+
+44
+00:03:15,600 --> 00:03:19,240
+some bacteria قال لي some مش all لإنه قلنا إن
+
+45
+00:03:19,240 --> 00:03:22,800
+الاسبار عبارة عن special component مش كل أنواع
+
+46
+00:03:22,800 --> 00:03:25,700
+البكتيريا بتكونه، في بكتيريا عندها القدرة على
+
+47
+00:03:25,700 --> 00:03:28,980
+تكوين الاسبار، spar farming bacteria وفي عند
+
+48
+00:03:28,980 --> 00:03:33,660
+بكتيريا ما عندها القدرة إنها تكون الاسبار، non
+
+49
+00:03:33,660 --> 00:03:38,940
+spar farming bacteriafor survival during adverse
+
+50
+00:03:38,940 --> 00:03:43,180
+environmental conditions بقولك تستخدم للحماية و
+
+51
+00:03:43,180 --> 00:03:47,920
+للبقاء survival اللي هي البقاء في الظروف البيئية
+
+52
+00:03:47,920 --> 00:03:52,020
+الصعبة على سبيل المثال في nutrient limitation في
+
+53
+00:03:52,020 --> 00:03:57,300
+حال نقص الأغذية or extreme environmentفي حال كان
+
+54
+00:03:57,300 --> 00:04:02,060
+عندي ظروف بيئية صعبة زي الجفاف الـ Spore are
+
+55
+00:04:02,060 --> 00:04:05,860
+resistant to heat and chemical بقوللي ان الـ Spore
+
+56
+00:04:05,860 --> 00:04:10,980
+مقاوم للحرارة و للمواد الكيميائية ليش؟ because of
+
+57
+00:04:10,980 --> 00:04:14,260
+a tough outer covering made of the protein keratin
+
+58
+00:04:14,260 --> 00:04:18,340
+بقولنا لأن هو محاط ببروتين اسمه الـ keratin يبقى
+
+59
+00:04:18,340 --> 00:04:22,390
+هذا مصطلح الـ keratin مهميبقى وجود الكيراتين هو
+
+60
+00:04:22,390 --> 00:04:25,970
+اللي بيخلّي الـ Spore مقاوم للحرارة وللمواد
+
+61
+00:04:25,970 --> 00:04:30,090
+الكيميائية من ضمن المواد الكيميائية الـ Stain
+
+62
+00:04:30,090 --> 00:04:34,730
+الصبغات يبقى the keratin also resist staining يبقى
+
+63
+00:04:34,730 --> 00:04:39,330
+الكيراتين هيكون مقاوم لل Staining للصباغة ال Spore
+
+64
+00:04:39,330 --> 00:04:44,630
+هيكون مقاومة للصباغة so extreme measuremust be
+
+65
+00:04:44,630 --> 00:04:48,050
+taken to stand a spar عشان هيك لازم انا اخد بعين
+
+66
+00:04:48,050 --> 00:04:54,530
+الاعتبار هذا الاشي لما بدي انا اصبغ ليه spar في
+
+67
+00:04:54,530 --> 00:04:59,350
+هاي ال slide هنتعرف على خصائص ال spar ال endospar
+
+68
+00:04:59,350 --> 00:05:03,930
+can remain dormant indefinitely not reproductive
+
+69
+00:05:03,930 --> 00:05:09,590
+بقوللي انه ال endospar بيضلوا خامل لمدة الحياة لكن
+
+70
+00:05:09,590 --> 00:05:13,170
+لو اتحسنت الظروف but germinate quickly when
+
+71
+00:05:13,700 --> 00:05:17,120
+appropriate trigger is applied لو اتحسنت الظروف
+
+72
+00:05:17,120 --> 00:05:21,220
+ممكن انه يسله جرمن عشان يتحول ال sport ل
+
+73
+00:05:21,220 --> 00:05:26,300
+vegetative cell لكن بيظلوا خامل فترة طويلة جدا مش
+
+74
+00:05:26,300 --> 00:05:31,480
+هيتكاثر non reproductive هو inactive قولنا ان هو
+
+75
+00:05:31,480 --> 00:05:35,740
+خامل dehydrated يعني منزوع منه الميه stable for
+
+76
+00:05:35,740 --> 00:05:40,560
+years قولناإنه بيظلوا استابل لسنوات عديدة وطويلة
+
+77
+00:05:40,560 --> 00:05:45,280
+ممكن في حالة حسنة الظروف حتى لو بعد مدة طويلة عنده
+
+78
+00:05:45,280 --> 00:05:49,260
+القدرة to germinate ويتحول ل vegetative cell
+
+79
+00:05:49,260 --> 00:05:53,700
+Indosperm differs significantly from the
+
+80
+00:05:53,700 --> 00:05:57,580
+vegetative or normally functioning cell بقولي إن
+
+81
+00:05:57,580 --> 00:06:01,100
+ال Indosperm بيختلف كليا عن ال vegetative cell
+
+82
+00:06:01,100 --> 00:06:06,000
+قلنا لinactive، dormant، لكن الـ vegetative cell
+
+83
+00:06:06,000 --> 00:06:09,840
+reproductive، تكاثرية الـ spar is stable for
+
+84
+00:06:09,840 --> 00:06:14,240
+years، لكن ال vegetative cell مش stable، هتمر بالـ
+
+85
+00:06:14,240 --> 00:06:19,640
+death phase، هتموت، هتمر في طور الموت بكتيريا can
+
+86
+00:06:19,640 --> 00:06:24,080
+form endospars in approximately six to eight hours
+
+87
+00:06:24,080 --> 00:06:27,980
+after being exposed to adverse conditionsبقول لي
+
+88
+00:06:27,980 --> 00:06:31,800
+البكتيريا بتقدر اتكون لـ Spore طبعا في حال أصبحت
+
+89
+00:06:31,800 --> 00:06:37,100
+الظروف غير مناسبة للنمو تقريبا بعد 6 ل 8 ساعات من
+
+90
+00:06:37,100 --> 00:06:41,000
+تعرضها لهذه الظروف السيئة يعني عملية الـ
+
+91
+00:06:41,000 --> 00:06:45,340
+Sporulation تحول ال vegetative cell لIndospore
+
+92
+00:06:45,340 --> 00:06:51,440
+بتتم بعد 6 ل 8 ساعات من تعرضها لظروف germinate
+
+93
+00:06:51,440 --> 00:06:57,130
+into a vegetative cell with 1.5 power لكن بقولنافي
+
+94
+00:06:57,130 --> 00:07:02,150
+حال تحسنت الظروف الـ spar هتتحول لـvegetative cell
+
+95
+00:07:02,150 --> 00:07:06,270
+خلال ساعة و نص يبقى عملية ال germination بتتم بعد
+
+96
+00:07:06,270 --> 00:07:10,390
+ساعة و نص من تحسن الظروف عشان هيك في أول جملة
+
+97
+00:07:10,390 --> 00:07:14,850
+قاللي انه germinate quickly عملية ال germination
+
+98
+00:07:14,850 --> 00:07:18,410
+بتسير بسرعة بعد ساعة و نص من تحسن الظروف لكن ال
+
+99
+00:07:18,410 --> 00:07:23,050
+sparulation بتتسير بعد 6 ل 8 ساعات من تعرضها
+
+100
+00:07:23,050 --> 00:07:27,680
+للظروف السيئة يعني ال germination بتكونأسرع من الـ
+
+101
+00:07:27,680 --> 00:07:31,960
+Sparulation Spar
+
+102
+00:07:31,960 --> 00:07:36,580
+formed by gram positive bacteria و احنا قلنا انه
+
+103
+00:07:36,580 --> 00:07:40,180
+مش كل البكتيريا بتكون لـ Spar أشهر البكتيريا اللي
+
+104
+00:07:40,180 --> 00:07:45,160
+بتكون لـ Spar هي البصلص و الكلستريديوم البصلص و
+
+105
+00:07:45,160 --> 00:07:49,670
+الكلستريديوم هدول gram positive bacteriaالـ Gram
+
+106
+00:07:49,670 --> 00:07:53,750
+-positive Bacilli الشاب تبعهم Bacilli تمام
+
+107
+00:07:53,750 --> 00:07:56,190
+البكتيريا لـ Spar farming بقولنا
+
+108
+00:07:56,190 --> 00:07:59,730
+البسيلسوليكلوستريديوم هي Gram-positive بكتيريا هذا
+
+109
+00:07:59,730 --> 00:08:03,090
+لا يعني أن كل Gram-positive هي Spar farming في
+
+110
+00:08:03,090 --> 00:08:06,310
+عندي بكتيريا Gram-positive لكن لا Spar farming
+
+111
+00:08:06,310 --> 00:08:10,570
+بدنا نحكي عن أشهر البكتيريا لـ Spar farming هنتكلم
+
+112
+00:08:10,570 --> 00:08:15,440
+عنهم بشكل مختصرأول بكتيريا الـ Bacillus anthracis
+
+113
+00:08:15,440 --> 00:08:21,000
+اللي بتسبب الـ Anthrax الجمرة الخبيثة الإصابة بهذه
+
+114
+00:08:21,000 --> 00:08:24,720
+البكتيريا ممكن تكون عن طريق ال injection أو ال
+
+115
+00:08:24,720 --> 00:08:27,780
+inhalation الاستنشاق استنشاق الـ Bacillus
+
+116
+00:08:27,780 --> 00:08:32,700
+anthracis sport الاستنشاق كانوا يستخدموها في
+
+117
+00:08:32,700 --> 00:08:36,590
+الحروب البيولوجيةبروحوا بيجيبوا ضرف و بيحطوا فيه
+
+118
+00:08:36,590 --> 00:08:39,690
+الـ spar تبع البكتيريا و بيروحوا بيبعتوها لقى
+
+119
+00:08:39,690 --> 00:08:43,950
+الدولة المعادية فلما يصل الضرف بيفتحه بيشمه
+
+120
+00:08:43,950 --> 00:08:49,250
+بيستنشق فبالتالي بيصاب بي ال anthrax بي الجمرة
+
+121
+00:08:49,250 --> 00:08:53,530
+الخبيثة بالنسبة لكلوستريديوم الكلوستريديوم في عندي
+
+122
+00:08:53,530 --> 00:08:57,590
+three species بيسببوا أمراض للإنسان أول واحد
+
+123
+00:08:57,590 --> 00:09:01,940
+الكلوستريديوم Botulinum اللي بيسبباللي بيسبب الـ
+
+124
+00:09:01,940 --> 00:09:06,220
+botulism اللي هو الـ food poisoning بيسبب هذا
+
+125
+00:09:06,220 --> 00:09:09,820
+بيصير في عيني انا لو شخص انصاب الكلستريديوم
+
+126
+00:09:09,820 --> 00:09:13,920
+بوتولينيوم بيصير في عينيه شلل ارتخائي عشان هيك
+
+127
+00:09:13,920 --> 00:09:19,340
+بيتم استخدامه في عمليات التجميل ال Botox بيتم حقد
+
+128
+00:09:19,340 --> 00:09:24,230
+منطقة مثلا حول العين عشان يصير في عيني ارتخاءفي
+
+129
+00:09:24,230 --> 00:09:28,170
+عندى نوع اخر من الـ Clostridium سبيشيز اخر من ال
+
+130
+00:09:28,170 --> 00:09:32,090
+Clostridium Clostridium Perifringes اللى بتسبب gas
+
+131
+00:09:32,090 --> 00:09:36,750
+gangrene اللى هى نقص وصول ال oxygen للانسجار
+
+132
+00:09:36,750 --> 00:09:42,190
+فبالتالي بيصير العضو لونه أسود Clostridium Tetani
+
+133
+00:09:42,190 --> 00:09:45,890
+اللى بتسبب ال tetanus او له اسم تانى ال fixed
+
+134
+00:09:45,890 --> 00:09:49,950
+smile او الكزاز بيكون ضغط على أسنانه بيصير عنده
+
+135
+00:09:49,950 --> 00:09:53,630
+شلل انقباضىيبقى الـ Clostridium تيتاني بيكون فيه
+
+136
+00:09:53,630 --> 00:09:57,210
+عندى شلل انقباضي لكن الـ Clostridium Botulinum
+
+137
+00:09:57,210 --> 00:10:02,710
+بيصير فيه عندى شلل ارتخالي هاي بالنسبة لأشهر
+
+138
+00:10:02,710 --> 00:10:06,130
+البكتيريا الـ Spar Forming هتتعرفوا أكتر عن كل
+
+139
+00:10:06,130 --> 00:10:09,530
+بكتيريا عن الـ pathogenesis طريقة إحدافها للمرض
+
+140
+00:10:09,530 --> 00:10:14,170
+لكن المطلوب تعرفوا أن هدول هم Spar Forming المطلوب
+
+141
+00:10:14,170 --> 00:10:23,650
+نعرف شو اسم المرض اللي بتسببه كل بكتيرياIndospore
+
+142
+00:10:23,650 --> 00:10:27,230
+location موقع الاندوسبورت position of the
+
+143
+00:10:27,230 --> 00:10:31,710
+endospore differs among bacterial species and is
+
+144
+00:10:31,710 --> 00:10:36,590
+useful in identification بقوللي أن موقع
+
+145
+00:10:36,590 --> 00:10:40,010
+الاندوسبورت بختلف من species لآخر ممكن يكون
+
+146
+00:10:40,010 --> 00:10:43,950
+central endospore يقع في المنتصف ممكن يكون sub
+
+147
+00:10:43,950 --> 00:10:47,450
+terminal قريب من الطرف ممكن يكون terminal
+
+148
+00:10:47,450 --> 00:10:51,670
+endosporeمن الأمثلة على الـ «Central» البصيلة
+
+149
+00:10:51,670 --> 00:10:55,090
+«Ceres» والبصيلة «Anthrax» «Terminal»
+
+150
+00:10:55,090 --> 00:10:58,470
+«Eclostridium» «Titanium» «Subterminal» البصيلة
+
+151
+00:10:58,470 --> 00:11:01,670
+«Subtules» يبقى في عندي إضافات البصيلة «Ceres»
+
+152
+00:11:01,670 --> 00:11:06,110
+والبصيلة «Subtules» أنا بعتبرهم «Spar Farming
+
+153
+00:11:06,110 --> 00:11:10,570
+Bacteria» احنا قلنا انه لـ «Gram Stain» هي أول
+
+154
+00:11:10,570 --> 00:11:14,790
+خطوة من خطوات تعريف البكتيريا لكن ما بقدر من خلال
+
+155
+00:11:14,790 --> 00:11:19,410
+«Gram Stain» فقط أتعرف على البكتيريابقول لي من
+
+156
+00:11:19,410 --> 00:11:22,390
+خلال الـ IndoSpar Stain أو الـ IndoSpar Location
+
+157
+00:11:22,390 --> 00:11:26,630
+ممكن يساعد في التعريف، كيف؟ على سبيل المثال، كأن
+
+158
+00:11:26,630 --> 00:11:31,890
+عندي بكتيريا X، بدي أنا أتعرف عليها أول خطوة عشان
+
+159
+00:11:31,890 --> 00:11:36,030
+أعمل تعريف، بعمل لـ Gram Stain عملت Gram Stain،
+
+160
+00:11:36,030 --> 00:11:39,790
+طلعت Gram Positive Bustle Life طيب، أنا أعمل لـ
+
+161
+00:11:39,790 --> 00:11:42,450
+Gram Positive Bustle Life يعني مجموعة كبيرة من
+
+162
+00:11:42,450 --> 00:11:45,650
+البكتيريا، بعرف أن في مجموعة ممكن تكون Spar
+
+163
+00:11:45,650 --> 00:11:49,580
+Formingوفيه ممكن يكون منها non-spar farming فروح
+
+164
+00:11:49,580 --> 00:11:56,020
+اتعملت الـ endospar stem طلعت من ال endospar stem
+
+165
+00:11:56,020 --> 00:12:00,220
+البكتيريا هي spar farming يبقى هستثنى كل البكتيريا
+
+166
+00:12:00,220 --> 00:12:04,680
+non-spar farming من ال gram positive bacilli هروح
+
+167
+00:12:04,680 --> 00:12:08,540
+افكر ب spar farming من ال gram positive bacilli
+
+168
+00:12:08,540 --> 00:12:11,860
+انا بعرف من ال spar farming ال gram positive
+
+169
+00:12:11,860 --> 00:12:17,160
+bacilli اللي هي البصلص والكلستريديومعشان افرق بين
+
+170
+00:12:17,160 --> 00:12:20,320
+البصلص و الـ Clostridium في فحص كيميا ايه هنتعرف
+
+171
+00:12:20,320 --> 00:12:24,740
+عليه في المعامل اللي جاي طيب انا هذا الفحص عملته
+
+172
+00:12:24,740 --> 00:12:27,860
+كانت النتيجة ان هاي البكتيريا بصلص طب فيه
+
+173
+00:12:27,860 --> 00:12:31,700
+اندوسبيشيز من البصلص كيف بدي اعرف اي species هاي
+
+174
+00:12:31,700 --> 00:12:35,560
+البكتيريا؟ بقوللي من خلال موقع الIndospar
+
+175
+00:12:35,560 --> 00:12:39,720
+الIndospar location انا سبقت كان الIndospar
+
+176
+00:12:39,720 --> 00:12:43,940
+location اللي هو sub terminal يبقى البكتيريا هي
+
+177
+00:12:43,940 --> 00:12:48,460
+بصلصيبقى هيك أنا من خلال الـ Position of Endospore
+
+178
+00:12:48,460 --> 00:12:54,720
+ساعدني في تعريف البكتيريا Endospore
+
+179
+00:12:54,720 --> 00:12:58,200
+shape شكل الـ Endospore ممكن يكون كراوي ممكن يكون
+
+180
+00:12:58,200 --> 00:13:02,440
+بيضاوي و ممكن يكون أسطوان في عندي أشكال مختلفة
+
+181
+00:13:02,440 --> 00:13:06,180
+بالنسبة للـ Endospore size بقولي أن الـ Endospore
+
+182
+00:13:06,180 --> 00:13:10,440
+can also be larger or smaller in diameter than the
+
+183
+00:13:10,440 --> 00:13:14,560
+vegetative cell Endospore ممكن يكون حجمهأكبر أو
+
+184
+00:13:14,560 --> 00:13:20,640
+أصغر من الـ vegetative cell في أول تلت بكتيريا أول
+
+185
+00:13:20,640 --> 00:13:23,300
+تلت صور هذه الصورة الأولى والتانية والتالتة
+
+186
+00:13:23,300 --> 00:13:26,760
+مانلاحظ انه ال Indus Bar أصغر من ال vegetative
+
+187
+00:13:26,760 --> 00:13:30,360
+cell لكن في الصورة الرابعة والخامسة والسادسة ال
+
+188
+00:13:30,360 --> 00:13:34,480
+Indus Bar كان أكبر من ال vegetative cell وسبب
+
+189
+00:13:34,480 --> 00:13:38,360
+الانتفاخ لل vegetative cell في المنطقة المحيطة بال
+
+190
+00:13:38,360 --> 00:13:41,600
+Indus Bar مش في كل ال vegetative cell فقط المنطقة
+
+191
+00:13:41,600 --> 00:13:45,750
+المحيطة بال Indus Barthose that are larger in
+
+192
+00:13:45,750 --> 00:13:48,990
+diameter will produce an area of swelling in the
+
+193
+00:13:48,990 --> 00:13:52,750
+vegetative cell هيسبب لانتفاخ لال vegetative cell
+
+194
+00:13:52,750 --> 00:13:56,210
+الانتفاخ هيكون فقط في المنطقة المحيطة بالـ
+
+195
+00:13:56,210 --> 00:14:02,970
+Endospore طيب نتعرف هلأ على طريقة الصباغة الـ
+
+196
+00:14:02,970 --> 00:14:06,670
+Endospore Staining اللي هاسم تاني discover Fulton
+
+197
+00:14:06,670 --> 00:14:11,650
+Method لازم نكون عارفينأي استان إذا إلها اسم تاني
+
+198
+00:14:11,650 --> 00:14:14,710
+نكون عارفين هل ده الاسم زي الـ Acid Fast Stain كان
+
+199
+00:14:14,710 --> 00:14:19,430
+إلها الـ Zel Nelson Stain لأنه ممكن في الامتحان
+
+200
+00:14:19,430 --> 00:14:22,530
+أنا أجيب أي سؤال بيتعلق بالـ Acid Fast Stain لكن
+
+201
+00:14:22,530 --> 00:14:25,510
+ما أكتب Acid Fast Stain أكتب الاسم التاني Zel
+
+202
+00:14:25,510 --> 00:14:29,410
+Nelson Stain فتكوني أنتي عارفة إن هي هاي هي نفس
+
+203
+00:14:29,410 --> 00:14:35,690
+الـ Acid Fast Stain كأي خطوة كأي استان في أي في
+
+204
+00:14:35,690 --> 00:14:39,480
+المعمل الـ Microbiology Lab أول خطوةSmear
+
+205
+00:14:39,480 --> 00:14:42,900
+Preparation الخطوة التانية Air Drying الخطوة
+
+206
+00:14:42,900 --> 00:14:48,000
+التالتة هي Fixation وعارفين كل تفاصيل هذه الخطوات
+
+207
+00:14:48,000 --> 00:14:52,400
+اللي اترقنا اليهم في معمل ال symbol STAIN الخطوة
+
+208
+00:14:52,400 --> 00:14:56,320
+الرابعة بتكون ال STAIN قولنا هي بتختلف حسب نوع ال
+
+209
+00:14:56,320 --> 00:15:02,200
+STAIN اليوم هنتكلم على ال Endospore STAIN قولنا في
+
+210
+00:15:02,200 --> 00:15:07,520
+بداية المعمل ان ال STAIN مقاوم للصبغة نتيجة وجود
+
+211
+00:15:07,520 --> 00:15:11,680
+الكيراتينإذا بتتذكروا في الـ Acid fast stain قلنا
+
+212
+00:15:11,680 --> 00:15:15,880
+الـ Mycobacterium مقاومة أيضًا للصبغة نتيجة وجود
+
+213
+00:15:15,880 --> 00:15:20,900
+الـ wax lipid لكن أنا قدرت أخترق ال wax lipid عن
+
+214
+00:15:20,900 --> 00:15:25,020
+طريق تعريض البكتيريا للـ steam ساعد على اختراق
+
+215
+00:15:25,020 --> 00:15:28,820
+اللي هو الـ Carbolfoxin اللي كانت primarily stain
+
+216
+00:15:28,820 --> 00:15:33,420
+للـ wax lipid وقدرت أني أنا أصبغهاهان في الاندو
+
+217
+00:15:33,420 --> 00:15:37,960
+سبارك نفس الفكرة السبارك بتتكون من كيراتين صعبة
+
+218
+00:15:37,960 --> 00:15:42,800
+الصباغة فبالتالي هستخدم لستيم حتى يكسر الروابط في
+
+219
+00:15:42,800 --> 00:15:47,740
+الكيراتين وبالتالي هيسهل اختراق الصبغة لداخل
+
+220
+00:15:47,740 --> 00:15:51,060
+السبارك لكن في ال acid fast stain انا استخدمت
+
+221
+00:15:51,060 --> 00:15:53,720
+كاربولفوكسين ك primary stain مش هستخدم
+
+222
+00:15:53,720 --> 00:15:56,920
+كاربولفوكسين في الاندو سبارك كاربولفوكسين كانت
+
+223
+00:15:56,920 --> 00:16:01,590
+مميزاتها ان هيLipid soluble وهذا اللي ساعدها في
+
+224
+00:16:01,590 --> 00:16:05,990
+اختراق ال wax lipid في ال mycobacterium لكن في
+
+225
+00:16:05,990 --> 00:16:10,190
+الاسبوري ماعندي wax lipid عندي كيراتين هستخدم
+
+226
+00:16:10,190 --> 00:16:16,030
+Malachite green الملاكايت
+
+227
+00:16:16,030 --> 00:16:20,730
+جرين هي ال primary stain الملاكايت جرين بنعرف أنه
+
+228
+00:16:20,730 --> 00:16:26,850
+هي basic stainو معلومة إضافية أنها water soluble
+
+229
+00:16:26,850 --> 00:16:29,790
+يبقى نكتب هان ال primary stem
+
+230
+00:16:41,160 --> 00:16:44,480
+الـ Primary Stain الملقاقية Green وهي Water
+
+231
+00:16:44,480 --> 00:16:49,320
+Soluble يبقى عشان نحاول اننا نحفظ ونقارن ال Acid
+
+232
+00:16:49,320 --> 00:16:52,280
+Phosphate Stain استخدمت ال Primary Stain الكربو
+
+233
+00:16:52,280 --> 00:16:56,400
+الفوكسيني اللي تعتبر Lipid Soluble عشان ال wax
+
+234
+00:16:56,400 --> 00:16:59,900
+lipid الموجود في ال Mycobacterium لكن في ال Indus
+
+235
+00:16:59,900 --> 00:17:03,360
+Pore استخدمت ال Primary Stain الملقاقية Green اللي
+
+236
+00:17:03,360 --> 00:17:08,540
+هي Water Soluble ليش استخدمتها نعرف في ال slides
+
+237
+00:17:08,540 --> 00:17:12,810
+الجايةأول خطوة في الستان طبعا احنا قلنا spear
+
+238
+00:17:12,810 --> 00:17:16,050
+preparation and air drying heat fixation بعدها
+
+239
+00:17:16,050 --> 00:17:19,770
+هصبغ هجيب بيكر احط فيه ميه اخليها تغلي لما تغلي
+
+240
+00:17:19,770 --> 00:17:23,910
+هحط ال slide على طرف البيكر بغمر ال slide بال
+
+241
+00:17:23,910 --> 00:17:29,030
+primary stand الملاكايت ال green هستنى من 3 ل 5
+
+242
+00:17:29,030 --> 00:17:35,570
+دقائق و بعد ما تنتهي الخمس دقائق طبعا هستنى هخليها
+
+243
+00:17:35,570 --> 00:17:39,840
+تبردالـ steam هيعمل enhance ملاكاية إيجرين
+
+244
+00:17:39,840 --> 00:17:44,560
+بنتريشون هيساعد على اختراق الملاكاية إيجرين الصبغة
+
+245
+00:17:44,560 --> 00:17:50,100
+للكيراتين للسبوع طبعا بقولي انا لو جفت الصبغة نفس
+
+246
+00:17:50,100 --> 00:17:52,920
+في ال acid phosphate بضيف الصبغة لأنها يتكون
+
+247
+00:17:52,920 --> 00:17:57,740
+كريستال لونه أخضر بعد ما اتخلص الخمس دقائق بخليها
+
+248
+00:17:57,740 --> 00:18:02,260
+تبرد يبقى الخطوة التانية cooling هعمل cooling وهي
+
+249
+00:18:02,260 --> 00:18:06,330
+الخطوة مهمة جدا لأن لو ما خلتها تبردمدة كفاية
+
+250
+00:18:06,330 --> 00:18:11,210
+هتأثر على النتائج ال cooling قولنا act as more
+
+251
+00:18:11,210 --> 00:18:15,050
+than يبقى ال cooling هيكون act as more than
+
+252
+00:18:24,320 --> 00:18:29,160
+تمام ال cooling يعتبر ك-mordant لأنه من خلال ال ال
+
+253
+00:18:29,160 --> 00:18:32,580
+cooling هيكون ك-mordant لأنه من خلال ال heat أو ال
+
+254
+00:18:32,580 --> 00:18:36,860
+steam اتكسرت الروابط في الكيراتين قدرت الصبغة
+
+255
+00:18:36,860 --> 00:18:41,080
+الملاكاية ال green تخترق ال spar حتى أثبت
+
+256
+00:18:41,080 --> 00:18:44,760
+الملاكاية ال green في ال spar بخليها تبرد لأنه ال
+
+257
+00:18:44,760 --> 00:18:48,280
+cooling هيعمل re-naturation للروابط بالتالي الصبغة
+
+258
+00:18:48,280 --> 00:18:53,280
+هتثبتبعد ال cooling gently rinse the slide with
+
+259
+00:18:53,280 --> 00:18:56,760
+water الخطوة بعد ما احنا بستخدم ال primary stain
+
+260
+00:18:56,760 --> 00:19:01,480
+ماردان بعدين بستخدم ال decolorizer ال decolorizer
+
+261
+00:19:01,480 --> 00:19:05,460
+في ال Indosporous stain هو ال water يبقى ال
+
+262
+00:19:05,460 --> 00:19:07,220
+decolorizer هو ال water
+
+263
+00:19:22,030 --> 00:19:26,530
+الـ water هعتبره ك decalorizer طب ليش؟ احنا قلنا
+
+264
+00:19:26,530 --> 00:19:31,250
+ان ال primary stain هي الملاكايت green واتفقنا على
+
+265
+00:19:31,250 --> 00:19:35,710
+معلومة ان الملاكايت green هي water soluble يبقى
+
+266
+00:19:35,710 --> 00:19:39,810
+الميه هتزيل الملاكايت green هتزيل الصبغة بدون
+
+267
+00:19:39,810 --> 00:19:44,830
+الحاجة لأي مادة كيميائية يبقى عرفنا ليش انا اخترت
+
+268
+00:19:44,830 --> 00:19:48,890
+بالذات الملاكايت green ك primary stain لأنها water
+
+269
+00:19:48,890 --> 00:19:54,160
+solubleو احنا ما استخدمنا اي مادة كيميائية اعتبرنا
+
+270
+00:19:54,160 --> 00:19:58,440
+ال water هو ال decalryzer بعدها هضيف ال
+
+271
+00:19:58,440 --> 00:20:04,800
+counterstain للصفرانين لمدة من 1 ل 2 من 1 ل 2
+
+272
+00:20:04,800 --> 00:20:09,800
+minute بعدها هغسل بالwater هعمل air drying على ال
+
+273
+00:20:09,800 --> 00:20:12,920
+oil immersion يبقى الصفرانين هي counterstain
+
+274
+00:20:38,790 --> 00:20:43,270
+يبقى الصفرانين هي counter-stain قلنا الخطوة الأولى
+
+275
+00:20:43,270 --> 00:20:45,850
+اللى هى السمير ال preparation الخطوة التانى are
+
+276
+00:20:45,850 --> 00:20:48,970
+drying heat fixation ال primary stain الملاكايت
+
+277
+00:20:48,970 --> 00:20:53,670
+جرين اللى هى water soluble لكن هعرضها ل steam عشان
+
+278
+00:20:53,670 --> 00:20:57,230
+يزيد اختراق الملاكايت جرين ل ال spar أو ل
+
+279
+00:20:57,230 --> 00:21:01,430
+الكيراتين هعمل cooling mordant بعدها ال
+
+280
+00:21:01,430 --> 00:21:05,730
+decolorizer بى ال water وعرفنا السبب لأن الصفقة
+
+281
+00:21:05,730 --> 00:21:10,030
+الملاكايت جرين water soluble هعمل counterستان اللي
+
+282
+00:21:10,030 --> 00:21:19,070
+هي هتكون الصفرانين هذه بالنسبة للخطوات بالنسبة
+
+283
+00:21:19,070 --> 00:21:23,330
+لهذه الصورة عملني مقارنة بين Spar farming bacteria
+
+284
+00:21:23,330 --> 00:21:25,870
+و non spar farming bacteria ال Spar farming
+
+285
+00:21:25,870 --> 00:21:30,390
+bacteria البكتيريا فيه جواها endospar لكن ال non
+
+286
+00:21:30,390 --> 00:21:35,320
+spar farming ما بتحتوي على Spar سواء كانتSpar
+
+287
+00:21:35,320 --> 00:21:38,600
+farming او non spar farming البكتيريا و الـ spar
+
+288
+00:21:38,600 --> 00:21:45,280
+هيكونوا قبل الصباغة transparent هضيف
+
+289
+00:21:45,280 --> 00:21:48,040
+ال primary stain لملكات الـ green أكيد البكتيريا
+
+290
+00:21:48,040 --> 00:21:51,620
+بكل سهولة هتاخد الصبغة لأنها basic stain لكن ال
+
+291
+00:21:51,620 --> 00:21:55,220
+spar قلنا في كيراتين صعب انه ينصبخ عشان هيك
+
+292
+00:21:55,220 --> 00:22:00,620
+بنعرضها ل steam هذا ال steam هيعمل او هيساعد على
+
+293
+00:22:00,620 --> 00:22:05,120
+اختراق الملكات ال green لل sparهعمل cooling هيعمل
+
+294
+00:22:05,120 --> 00:22:08,100
+renaturation للروابط اللى اتكسرت في الكيراتين اللى
+
+295
+00:22:08,100 --> 00:22:12,880
+موجود اللى فى ال spar بعدها هغسل بالwater
+
+296
+00:22:12,880 --> 00:22:17,080
+decolorization with water بقولنا اللون هيروح من
+
+297
+00:22:17,080 --> 00:22:21,500
+وين from the cell بطنات ال spar hence سؤال مهم لش
+
+298
+00:22:21,500 --> 00:22:25,450
+اللون راح من البكتيريا وضلوا فى ال sparليش الـ
+
+299
+00:22:25,450 --> 00:22:29,390
+Lawn دلوا بالـ spar لأن الـ spar في مادة الكيراتين
+
+300
+00:22:29,390 --> 00:22:32,790
+احنا كسرنا الروابط من خلال الـ steam قعدنا الروابط
+
+301
+00:22:32,790 --> 00:22:37,190
+من خلال ال cooling تبتت الصبغة عشان هيك مهم جدا ال
+
+302
+00:22:37,190 --> 00:22:41,070
+cooling لو انا ماعملت cooling مدة كافية ممكن اللان
+
+303
+00:22:41,070 --> 00:22:45,610
+يروح حتى من ال spar و يطلع عندي false result
+
+304
+00:22:45,610 --> 00:22:49,410
+الخلية البكتيريا راح منها اللان لأن ال steel wall
+
+305
+00:22:49,410 --> 00:22:54,310
+تبعها ضعف من ال steam من الحرارةوهى اصلا ما عندها
+
+306
+00:22:54,310 --> 00:22:58,490
+كيراتين تقدر تحتفظ باللون فلما انا غسلت الملاكايت
+
+307
+00:22:58,490 --> 00:23:02,210
+جرين الصبغة بالواتر سهل انها تروح لأنها water
+
+308
+00:23:02,210 --> 00:23:06,590
+soluble بعدها هضيف ال counterstain الصفرانين اكيد
+
+309
+00:23:06,590 --> 00:23:09,990
+هتاخد اللون اللى نزال منها اللون يعني البكتيرى هي
+
+310
+00:23:09,990 --> 00:23:14,070
+اللى هتاخد اللون الـ spar هيظل لونها أخضر يبقى اذا
+
+311
+00:23:14,070 --> 00:23:17,170
+كانت الخلية كلها لونها أحمر هي non spar farming
+
+312
+00:23:17,170 --> 00:23:21,370
+اذا كانت الخلية لونها أحمر والـ spar أخضر يبقى هي
+
+313
+00:23:21,370 --> 00:23:26,560
+spar farmingفين دي ملاحظة اللي قلناها انه الـ
+
+314
+00:23:26,560 --> 00:23:30,280
+water في الـ sparse tanning اعتبرته ك decalorizer
+
+315
+00:23:30,280 --> 00:23:35,100
+لأنه الملاكايت green بيحتوي على copper salt is
+
+316
+00:23:35,100 --> 00:23:38,260
+water soluble احنا قلنا الملاكايت green هي water
+
+317
+00:23:38,260 --> 00:23:41,360
+soluble بالتحديد ايش المكون اللي في الملاكايت
+
+318
+00:23:41,360 --> 00:23:45,680
+green اللي هو water soluble ال copper salt بالتالي
+
+319
+00:23:45,680 --> 00:23:50,100
+سهل انه يزيل الصبغة عن ال water سهل انه يزيل
+
+320
+00:23:50,100 --> 00:23:55,890
+الصبغة عن الخلية البكتيريةvegetative cell have
+
+321
+00:23:55,890 --> 00:23:59,470
+been distributed by heat because of this reason
+
+322
+00:23:59,470 --> 00:24:02,870
+the malachite green runs easily from the
+
+323
+00:24:02,870 --> 00:24:05,270
+vegetative cell بقول لي أن ال vegetative cell
+
+324
+00:24:05,270 --> 00:24:08,470
+البكتيريا اتدمرت بواسطة ال heat أو ال steam
+
+325
+00:24:08,470 --> 00:24:13,510
+بالتالي سهل على الصبغة أنها تروح من ال vegetative
+
+326
+00:24:13,510 --> 00:24:17,950
+cell بدون الحاجة لأي مادة كيميائية لأن ال
+
+327
+00:24:17,950 --> 00:24:24,650
+malachite green هي water solubleهذه الخطوات بسوق
+
+328
+00:24:24,650 --> 00:24:30,310
+بيه الصور نفس اللي شرحناها مافي اي اضافات هاي
+
+329
+00:24:30,310 --> 00:24:33,870
+بالنسبة لل result النتيجة ال vegetatives اللي
+
+330
+00:24:33,870 --> 00:24:37,470
+ملونة باللون ال pink لكن ال endospar متلون باللون
+
+331
+00:24:37,470 --> 00:24:41,690
+الأخضر فى عندي hand freeze bar متلونة باللون
+
+332
+00:24:41,690 --> 00:24:44,570
+الأخضر وعندي ال vegetatives اللي متلونة باللون ال
+
+333
+00:24:44,570 --> 00:24:48,530
+pinkفي هذه الصورة في عندي Indospar متلونة باللون
+
+334
+00:24:48,530 --> 00:24:52,210
+الأخضر والسل متلونة باللون الأحمر وفي عندي
+
+335
+00:24:52,210 --> 00:24:56,770
+Efrespar متلونة باللون الأخضر هيك بنكون خلصنا أول
+
+336
+00:24:56,770 --> 00:25:00,670
+special component اللي هو ال Indospar تعرفنا على
+
+337
+00:25:00,670 --> 00:25:02,670
+ال Indospar Stemming
+