11982
document
S-phase kinase-associated protein 2 expression in laryngeal squamous cell carcinomas and its prognostic implications . The F-box protein S-phase kinase-associated protein 2 ( Skp2 ) positively regulates the G1-S transition by controlling the stability of several G1 regulators , such as the cell cycle inhibitor p27kip1 . However , the clinical significance of Skp2 in patients with laryngeal squamous cell carcinoma ( LSCC ) remains unknown . In this study , a potential distribution of Skp2 in LSCC and its clinical implications was investigated by an immunohistochemical study . Overall , Skp2 overexpression was observed in 36.7 % ( 37 of 102 ) patients and was significantly associated with lymph node metastasis ( p=0.002 ) and was inversely associated with p27kip1 expression ( p=0.026 ) . Survival analysis using the Kaplan-Meier method showed that Skp2 overexpression was significantly associated with shorter disease-free and overall survival ( p=0.0051 and p=0.0002 , respectively ) . When Skp2 expression and p27kip1 expression were combined , patients with both Skp2 overexpression and reduced expression of p27kip1 revealed poorest disease-free and overall survival as compared to the other cases ( p=0.0017 and p < 0.0001 , respectively ) . Additionally , in early stage ( I , II ) cases , Skp2 expression was also revealed to possess a significant prognostic factor in overall survival ( p=0.0234 ) , but not in disease-free survival ( p=0.2055 ) . By multivariate analysis using the Cox proportional hazards model , tumor grade , tumor size , clinical stage and Skp2 expression were independent prognostic factors both in disease-free and overall survival . These findings indicated that Skp2 expression was closely associated with tumor progression and represented an independent marker for prognosis of LSCC .
11983
Intervention_Pharmacological
S-phase kinase-associated protein
11984
Intervention_Pharmacological
S-phase kinase-associated protein
11985
Intervention_Pharmacological
Skp2
11986
Intervention_Physical
immunohistochemical study
11987
Intervention_Other
.
11988
Outcome_Physical
Skp2 overexpression
11989
Outcome_Mortality
Survival analysis
11990
Outcome_Mortality
disease-free and overall survival
11991
Outcome_Physical
Skp2 expression
11992
Outcome_Physical
p27kip1 expression
11993
Outcome_Physical
expression of p27kip1
11994
Outcome_Mortality
poorest disease-free
11995
Outcome_Mortality
overall survival
11996
Outcome_Mortality
overall survival ( p=0.0234 )
11997
Outcome_Mortality
disease-free survival
11998
Outcome_Physical
proportional hazards model , tumor grade , tumor size , clinical stage and Skp2 expression
11999
Outcome_Mortality
disease-free and overall survival .
12000
Outcome_Physical
Skp2 expression
12001
Participant_Condition
laryngeal squamous cell carcinomas
12002
Participant_Condition
LSCC
12003
Participant_Sample-size
37
12004
Participant_Sample-size
102