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document
Measurement of peptidase activity and evaluation of effectiveness of administration of minocycline for treatment of dogs with periodontitis . OBJECTIVE To determine clinical , enzymatic , and microbiologic effects of controlled-release localized administration of minocycline on dogs with periodontitis . ANIMALS Five adult Beagles with periodontitis . PROCEDURE After tooth scaling and root planing , 2 treatment , 1 placebo , and 1 control site were selected for each dog . Treatment sites ( n = 10 ) received a periodontal formulation of minocycline hydrochloride , placebo sites ( 5 ) received ointment without minocycline , and control sites ( 5 ) did not receive ointment . Treatments were administered 4 times at weekly intervals . Peptidase activity and clinical and microbiologic effects were evaluated and compared among sites for 17 weeks . RESULTS Bleeding of the gums on probing ( BOP ) and pocket depth ( PD ) improved at the treatment site and were maintained for 13 weeks after treatment . However , BOP and PD in placebo and control sites increased from weeks 9 to 17 Peptidase activity in the periodontal pocket decreased noticeably from week 1 to 17 , compared with baseline values for the treatment site . However , peptidase activity for placebo and control sites increased and were above baseline values on week 9 and week 13 , respectively . Total bacterial counts decreased by 90 % for treatment sites and remained at that value for 13 weeks . However , for placebo and control sites , bacterial counts increased and reached the baseline value on week 17 . CONCLUSIONS AND CLINICAL RELEVANCE Increased peptidase activity is correlated with the progression of periodontitis in dogs . Treatment with minocycline , using a localized delivery system , was effective in dogs for at least 13 weeks after cessation of drug administration .
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Intervention_Pharmacological
minocycline
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Intervention_Pharmacological
minocycline
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Intervention_Pharmacological
periodontal formulation of minocycline hydrochloride
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Intervention_Control
placebo
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Intervention_Pharmacological
received ointment without minocycline
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Intervention_Physical
control sites ( 5 ) did not receive ointment
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Intervention_Pharmacological
minocycline
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Outcome_Physical
peptidase activity
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Outcome_Physical
clinical , enzymatic
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Outcome_Adverse-effects
microbiologic effects
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Outcome_Adverse-effects
Peptidase activity and clinical and microbiologic effects
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Outcome_Physical
Bleeding of the gums on probing ( BOP ) and pocket depth ( PD ) improved
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Outcome_Physical
BOP
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Outcome_Physical
PD
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Outcome_Physical
Peptidase activity
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Outcome_Physical
peptidase activity
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Outcome_Physical
Total bacterial counts
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Outcome_Physical
bacterial counts
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Outcome_Physical
Increased peptidase activity
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Participant_Condition
dogs
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Participant_Condition
periodontitis
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Participant_Condition
dogs
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Participant_Condition
periodontitis
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Participant_Sample-size
Five
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Participant_Age
adult
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Participant_Condition
Beagles
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Participant_Condition
periodontitis
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Participant_Condition
periodontitis
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Participant_Condition
dogs