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document
Effects of maternal and dietary selenium ( Se-enriched yeast ) on the expression of p34 ( cdc2 ) and CyclinB1 of germ cells of their offspring in goats . The aim of the study was to evaluate the effect of selenium on the expression of p34 ( cdc2 ) and CyclinB1 ( two components of MPF regulating cell cycle ) of germ cells of their offspring in goats . A herd of 119 Taihang Black Goats , which was randomly divided into 4 treatments , received experimental diet with different Se levels ( from Se-enriched yeast ) for 174d . The four treatments , fed with a basal diet , were supplemented with 0 ( control ) , 0.5 , 2 and 4 mgkg⁻¹ DM Se . Testis samples were collected from the young male goats of each treatment group at the end of the study ( 30d after weaning ) for mRNA expression using real-time PCR and for protein expression by immunohistochemistry assay . Results show that a significant decrease was observed in mRNA expression of p34 ( cdc2 ) and CyclinB1 in the testis of Se-deficient ( Group 1 ) and Se-excess ( Group 4 ) animals compared with that in Groups 2 and 3 . However , no significant changes were found in mRNA expression of p34 ( cdc2 ) between Se-deficient ( Group 1 ) and Se-excess ( Group 4 ) . Also the immunohistochemistry assay detected similar results of protein expression of these two genes . These results suggest , that maternal and dietary Se-induced oxidative stress can modulate the mRNA and protein expression of the cell cycle related genes ( p34 ( cdc2 ) and CyclinB1 ) in the testis of their offspring . In addition , Se deficiency and Se excess could prevent the completion of the cell cycle .
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Intervention_Pharmacological
dietary selenium ( Se-enriched yeast )
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Intervention_Pharmacological
selenium
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Intervention_Pharmacological
experimental diet with different Se levels
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Participant_Condition
expression of p34 ( cdc2 ) and CyclinB1 of germ cells of their offspring in goats .
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Participant_Sample-size
119