24994
document
GH effect on enzyme activity of 11betaHSD in abdominal obesity is dependent on treatment duration . OBJECTIVE In the past years the interaction of GH and 11beta hydroxysteroid dehydrogenase ( 11betaHSD ) in the pathogenesis of central obesity has been suggested . DESIGN We studied the effects of 9 months of GH treatment on 11betaHSD activity and its relationship with body composition and insulin sensitivity in 30 men with abdominal obesity , aged 48-66 years , in a randomised , double-blind , placebo-controlled trial . METHODS Urinary steroid profile was used to estimate 11betaHSD type 1 and 2 ( 11betaHSD1 and 11betaHSD2 ) activities . Abdominal s.c. and visceral adipose tissues were measured using computed tomography . Glucose disposal rate ( GDR ) obtained during a euglycaemic-hyperinsulinaemic glucose clamp was used to assess insulin sensitivity . RESULTS In the GH-treated group the 11betaHSD1 activity decreased transiently after 6 weeks ( P < 0.01 ) whereas 11betaHSD2 increased after 9 months of treatment ( P < 0.05 ) . Between 6 weeks and 9 months , GDR increased and visceral fat mass decreased . Changes in 11betaHSD1 correlated with changes in visceral fat mass between baseline and 6 weeks . There were no significant correlations between 11betaHSD1 and 11betaHSD 2 and changes in GDR . DISCUSSION The study demonstrates that short- and long-term GH treatment has different effects on 11betaHSD1 and 11betaHSD2 activity . Moreover , the data do not support that long-term metabolic effects of GH are mediated through its action on 11betaHSD .
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Intervention_Pharmacological
GH
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Intervention_Pharmacological
GH treatment
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Intervention_Control
placebo-controlled
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Intervention_Physical
Abdominal s.c.
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Intervention_Physical
visceral adipose tissues
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Intervention_Physical
computed tomography
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Outcome_Physical
enzyme activity of 11betaHSD
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Outcome_Physical
body composition and insulin sensitivity
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Outcome_Physical
11betaHSD type 1 and 2 ( 11betaHSD1 and 11betaHSD2 ) activities
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Outcome_Physical
Abdominal s.c.
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Outcome_Physical
visceral adipose tissues
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Outcome_Physical
Glucose disposal rate ( GDR )
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Outcome_Physical
insulin sensitivity
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Outcome_Physical
11betaHSD1 activity
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Outcome_Physical
GDR
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Outcome_Physical
visceral fat mass
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Outcome_Physical
11betaHSD1
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Outcome_Physical
visceral fat mass
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Outcome_Physical
11betaHSD1
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Outcome_Physical
11betaHSD 2
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Outcome_Physical
11betaHSD1
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Outcome_Physical
11betaHSD2 activity
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Participant_Sample-size
30
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Participant_Sex
men
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Participant_Condition
abdominal obesity
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Participant_Age
aged 48-66 years