47553
document
Tomato paste rich in lycopene protects against cutaneous photodamage in humans in vivo : a randomized controlled trial . BACKGROUND Previous epidemiological , animal and human data report that lycopene has a protective effect against ultraviolet radiation ( UVR ) -induced erythema . OBJECTIVES We examined whether tomato paste -- rich in lycopene , a powerful antioxidant -- can protect human skin against UVR-induced effects partially mediated by oxidative stress , i.e . erythema , matrix changes and mitochondrial DNA ( mtDNA ) damage . METHODS In a randomized controlled study , 20 healthy women ( median age 33 years , range 21-47 ; phototype I/II ) ingested 55 g tomato paste ( 16 mg lycopene ) in olive oil , or olive oil alone , daily for 12 weeks . Pre- and postsupplementation , UVR erythemal sensitivity was assessed visually as the minimal erythema dose ( MED ) and quantified with a reflectance instrument . Biopsies were taken from unexposed and UVR-exposed ( 3 × MED 24 h earlier ) buttock skin pre- and postsupplementation , and analysed immunohistochemically for procollagen ( pC ) I , fibrillin-1 and matrix metalloproteinase ( MMP ) -1 , and by quantitative polymerase chain reaction for mtDNA 3895-bp deletion . RESULTS Mean ± SD erythemal D ( 30 ) was significantly higher following tomato paste vs. control ( baseline , 26·5 ± 7·5 mJ cm ( -2 ) ; control , 23 ± 6·6 mJ cm ( -2 ) ; tomato paste , 36·6 ± 14·7 mJ cm ( -2 ) ; P = 0·03 ) , while the MED was not significantly different between groups ( baseline , 35·1 ± 9·9 mJ cm ( -2 ) ; control , 32·6 ± 9·6 mJ cm ( -2 ) ; tomato paste , 42·2 ± 11·3 mJ cm ( -2 ) ) . Presupplementation , UVR induced an increase in MMP-1 ( P = 0·01 ) and a reduction in fibrillin-1 ( P = 0·03 ) . Postsupplementation , UVR-induced MMP-1 was reduced in the tomato paste vs. control group ( P = 0·04 ) , while the UVR-induced reduction in fibrillin-1 was similarly abrogated in both groups , and an increase in pCI deposition was seen following tomato paste ( P = 0·05 ) . mtDNA 3895-bp deletion following 3 × MED UVR was significantly reduced postsupplementation with tomato paste ( P = 0·01 ) . CONCLUSIONS Tomato paste containing lycopene provides protection against acute and potentially longer-term aspects of photodamage .
47554
Intervention_Pharmacological
Tomato paste rich in lycopene
47555
Intervention_Pharmacological
tomato paste -- rich in lycopene
47556
Intervention_Other
55 g tomato paste ( 16 mg lycopene ) in olive oil , or olive oil alone , daily for 12 weeks
47557
Intervention_Physical
.
47558
Outcome_Physical
cutaneous photodamage
47559
Outcome_Physical
ultraviolet radiation ( UVR ) -induced erythema
47560
Outcome_Physical
erythemal sensitivity
47561
Participant_Sample-size
20
47562
Participant_Sex
women
47563
Participant_Age
median age 33 years
47564
Participant_Age
range 21-47