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document
Effect of topical rh-TGF-beta 1 on second intention wound healing in horses . OBJECTIVE To investigate the effects on wound healing of transforming growth factor-beta 1 as a topical treatment to full-thickness , excisional wounds of the distal limb of horses . DESIGN A randomised block study using four horses , each with wounds assigned to four treatment groups . ANIMALS Four adult Standardbred geldings . PROCEDURE Four , 4 cm2 , full-thickness wounds were created on the dorsomedial and dorsolateral aspect of the metacarpus or metatarsus of each limb of four horses , giving a total of 64 wounds . For each limb , wounds were randomly assigned to four treatment groups : no treatment ( control ) , carrier ( Methyl Cellulose gel ) , 50 ng/wound rhTGF-beta 1 in carrier , and 500 ng/wound rhTGF-beta 1 in carrier . Wounds were treated on day 0 and day 8 . Effects of treatment were evaluated on the basis of the presence of exuberant granulation tissue requiring excision , number of times excision was required , total wound area , area of epithelialisation , area of granulation , and histological evaluation of biopsy samples of wounds on day 8 and excised wounds on day 21 . RESULTS Topical application of TGF-beta 1 at the two concentrations studied had no significant effect on the total area of wounds ( P = 0.7 ) , the area of granulation tissue ( P = 0.78 ) , the area of epithelialisation ( P = 0.92 ) , histological assessment or subjective clinical assessment of wounds . CONCLUSION TGF-beta 1 had no beneficial effects on wound healing . Additional trials are needed to test if it has value for wound treatment in horses .
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Intervention_Pharmacological
rh-TGF-beta 1
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Intervention_Pharmacological
growth factor-beta 1
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Intervention_Control
no treatment
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Intervention_Pharmacological
carrier ( Methyl Cellulose gel )
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Intervention_Pharmacological
50 ng/wound rhTGF-beta 1 in carrier ,
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Intervention_Pharmacological
500 ng/wound rhTGF-beta 1 in carrier
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Intervention_Pharmacological
TGF-beta 1
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Outcome_Physical
second intention wound healing
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Outcome_Physical
presence of exuberant granulation tissue requiring excision , number of times excision was required , total wound area , area of epithelialisation , area of granulation , and histological evaluation of biopsy samples of wounds
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Outcome_Physical
total area of wounds
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Outcome_Physical
the area of granulation tissue
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Outcome_Physical
area of epithelialisation
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Outcome_Physical
subjective clinical assessment of wounds .
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Outcome_Physical
wound healing
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Participant_Condition
second intention wound healing
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Participant_Sample-size
four