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document
Enhanced induction of apoptosis in lung adenocarcinoma after preoperative chemotherapy with tegafur and uracil ( UFT ) . PURPOSE To determine if the preoperative administration of tegafur and uracil ( UFT ) to patients with lung adenocarcinoma could induce apoptosis . METHODS We conducted a randomized prospective study on 30 patients with lung adenocarcinoma , divided into two groups of 15 patients each . One group received UFT 600 mg/day preoperatively for 7 consecutive days and a control group received no chemotherapy or radiotherapy . The apoptotic index ( AI ) was determined by the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate biotin nick end-labeling ( TUNEL ) method . Expression of Ki-67 was examined by immunohistochemical staining . The concentration of 5-fluorouracil ( 5-FU ) in tumor tissue was measured by chemical assay . RESULTS The AI of lung adenocarcinoma cells increased significantly in the UFT-treated group but not in the control group . A significant positive correlation was seen between the AI and the 5-FU concentrations in the tumor tissue . CONCLUSIONS The continuous oral administration of UFT for 7 days preoperatively resulted in enhanced apoptosis and a significant positive correlation between the AI and 5-FU concentrations in lung adenocarcinoma . Therefore , it may be possible to evaluate the effects of adjuvant chemotherapy based on the AI .
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Intervention_Pharmacological
tegafur
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Intervention_Pharmacological
uracil
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Intervention_Pharmacological
tegafur and uracil ( UFT )
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Intervention_Control
no chemotherapy or radiotherapy
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Outcome_Physical
induction
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Outcome_Other
apoptotic index ( AI )
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Outcome_Other
transferase-mediated deoxyuridine triphosphate biotin nick end-labeling ( TUNEL )
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Outcome_Physical
Expression of Ki-67
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Outcome_Physical
immunohistochemical staining
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Outcome_Physical
concentration of 5-fluorouracil ( 5-FU ) in tumor tissue
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Outcome_Physical
AI of lung adenocarcinoma cells
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Outcome_Physical
AI
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Outcome_Physical
the 5-FU concentrations
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Outcome_Physical
apoptosis
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Outcome_Physical
AI
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Outcome_Physical
5-FU
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Participant_Condition
lung adenocarcinoma
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Participant_Condition
patients with lung adenocarcinoma