75738
document
Actions of platelet activating factor ( PAF ) homologues and their combinations on neutrophil chemokinesis and cutaneous inflammatory responses in man . The inflammatory actions of synthetic C16:0 and C18:0 platelet activating factor ( PAF ) homologues , both alone and in combination , have been compared in an in vitro human neutrophil chemokinesis assay and by intradermal injection in human skin . In the chemokinesis assay , the maximum distance moved by neutrophils in the presence of C18:0 PAF was significantly greater than that seen with the C16:0 compound . A mixture of C16:0 and C18:0 PAFs in a ratio of 1:9 appeared to be more active than in a ratio of 3:1 . Intradermal injection of the C16:0 and C18:0 PAF homologues induced dose-dependent increases in weal volume and flare area responses which were not significantly different . Combination of these phospholipids in a ratio of 3:1 or 1:9 of C16:0 : C18:0 did not significantly alter the dose response curves . Thus , changes in the chain length of the alkyl substituent of synthetic PAF homologues and combination of these homologues , in ratios found in vivo or formed by leukocytes in vitro , did not alter the cutaneous inflammatory responses to PAF in man . The C18:0 homologue was , however , more active as a human neutrophil chemoattractant in vitro .
75739
Intervention_Pharmacological
platelet activating factor ( PAF ) homologues
75740
Intervention_Pharmacological
synthetic C16:0 and C18:0 platelet activating factor ( PAF ) homologues
75741
Intervention_Pharmacological
C18:0 PAF
75742
Intervention_Pharmacological
C16:0 compound .
75743
Intervention_Pharmacological
C16:0
75744
Intervention_Pharmacological
C18:0 PAFs
75745
Intervention_Pharmacological
C16:0 and C18:0 PAF homologues
75746
Intervention_Pharmacological
PAF homologues
75747
Intervention_Pharmacological
C18:0 homologue
75748
Outcome_Physical
maximum distance moved by neutrophils
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Outcome_Physical
weal volume
75750
Outcome_Physical
flare area responses
75751
Outcome_Physical
inflammatory responses to PAF
75752
Participant_Condition
neutrophil chemokinesis
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Participant_Condition
cutaneous inflammatory responses
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Participant_Sex
man
75755
Participant_Condition
neutrophil chemokinesis
75756
Participant_Condition
cutaneous inflammatory responses
75757
Participant_Sex
man