16482
document
Retrograde tracing and electrophysiological findings of collateral sprouting after end-to-side neurorrhaphy . The aim of this study was to seek more potent evidences of collateral sprouting for both motor and sensory nerve fibres after end-to-side neurorrhaphy using a modified double-labelling retrograde tracing method and to investigate the function of regenerated motor axons with electrophysiological evaluation . Four groups ( n=4 for each group ) were used : end-to-end coaptation ( six months postoperatively ) , end-to-side coaptation ( four months and six months postoperatively ) and normal control . Two fluorescent tracers ( true blue and diamidino yellow ) were applied to the proximal ends of tibial and common peroneal nerves , respectively after four or six months of nerve coaptation . Five days later , we only found single-labelled motor and sensory neurons in the normal and end-to-end coaptation groups , while some dual-labelled neurons can be identified in end-to-side coaptation groups . Four months after surgery , the motor nerve conduction velocity in end-to-side coaptation was significantly slower than in the normal control . But no difference was found in the sixth month . These results suggest that end-to-side neurorrhaphy can induce the functional collateral sprouting of both motor and sensory axons in the peripheral nerve .
16483
Intervention_Physical
end-to-side coaptation
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Intervention_Control
normal control .
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Intervention_Other
fluorescent tracers
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Intervention_Physical
end-to-side neurorrhaphy
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Outcome_Physical
single-labelled motor and sensory neurons
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Outcome_Other
motor nerve conduction velocity in end-to-side
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Participant_Condition
after end-to-side neurorrhaphy .
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Participant_Condition
after end-to-side neurorrhaphy