92350
document
The effect of quality and amount of dietary fat on the susceptibility of low density lipoprotein to oxidation in subjects with impaired glucose tolerance . OBJECTIVES We examined the effects of a high fat diet rich in monounsaturated fat ( MUFA-diet ) and a moderate fat diet rich in polyunsaturated fat ( PUFA-diet ) on the susceptibility of LDL to oxidation . SUBJECTS 29 subjects with impaired glucose tolerance . METHODS After consuming a run-in diet [ 37 % of energy ( E % ) fat , 18 E % saturated fat ] for three weeks , subjects were randomly assigned either to a MUFA-diet ( 40 E % fat , 19 E % monounsaturated fatty acids ) or a PUFA-diet ( 34 E % fat , 10 E % polyunsaturated fat ) for eight weeks . The susceptibility of LDL to oxidation was measured by challenging LDL with hemin and H2O2 and measuring the time for the reaction to reach maximum velocity . Results are expressed as lag time to oxidation in minutes . RESULTS In the PUFA-diet group ( n = 15 ) lag time tended to decrease during the experimental diet ( 97 +/- 28 vs 90 +/- 25 min , mean +/- s.d. , P = 0.073 ) , whereas in the MUFA-diet group ( n = 14 ) there was no significant change ( lag time 96 +/- 24 vs 100 +/- 16 min , P = 0.408 ) . The mean change in lag time was -7 +/- 14 min ( -7.2 % ) in the PUFA-diet group and +4 +/- 16 min ( +4.0 % ) in the MUFA-diet group ( P = 0.029 , PUFA-diet group vs MUFA-diet group ) . The alpha-tocopherol concentration in LDL increased significantly ( P < 0.01 ) in both diet groups relative to the run-in diet period , but LDL particle score did not change in either of the diet groups during the dietary intervention . In subjects with impaired glucose tolerance a PUFA-rich diet with a moderate amount of fat tended to increase the susceptibility of LDL to oxidation as compared to a higher fat diet rich in MUFA . Furthermore , the negative mean change in lag time to oxidation found in the PUFA-diet group differed significantly from the slightly positive mean change found in the MUFA-diet group .
92351
Intervention_Pharmacological
high fat diet rich in monounsaturated fat ( MUFA-diet )
92352
Intervention_Pharmacological
moderate fat diet rich in polyunsaturated fat ( PUFA-diet )
92353
Intervention_Pharmacological
PUFA-rich
92354
Intervention_Pharmacological
MUFA
92355
Outcome_Other
effect
92356
Outcome_Physical
susceptibility of low density lipoprotein to oxidation
92357
Outcome_Physical
susceptibility of LDL to oxidation
92358
Outcome_Physical
susceptibility of LDL to oxidation
92359
Outcome_Other
time for the reaction to reach maximum velocity
92360
Outcome_Other
lag time to oxidation
92361
Outcome_Other
lag time
92362
Outcome_Other
mean change in lag time
92363
Outcome_Physical
alpha-tocopherol concentration in LDL
92364
Outcome_Physical
LDL particle score
92365
Outcome_Physical
susceptibility of LDL to oxidation
92366
Outcome_Other
lag time to oxidation
92367
Participant_Condition
impaired glucose tolerance
92368
Participant_Sample-size
29