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document
Synthesis of interleukin-1 beta in primary biliary cirrhosis : relationship to treatment with methotrexate or colchicine and disease progression . Primary biliary cirrhosis ( PBC ) is a chronic , progressive , cholestatic liver disease . Interleukin-1 beta ( IL-1 beta ) may play a role in the pathogenesis of PBC by contributing to altered immune function and fibrosis . Colchicine or methotrexate has some beneficial effects in the treatment of PBC , and also affects interleukin-1 ( IL-1 ) . Therefore , we prospectively studied the synthesis of IL-1 beta by peripheral blood mononuclear cells ( PBMC ) from 42 patients with PBC entered into a randomized , double-blind , double-dummy controlled trial of colchicine and methotrexate . PBMC obtained at entry , 6 , 12 , 18 , and 24 months were stimulated to produce IL-1 beta with phytohemagglutinin ( PHA ) , lipopolysaccharide ( LPS ) , Staphylococcus epidermidis , recombinant IL-2 , or mitochondrial antigen . Patients in the two treatment groups did not differ at entry in biochemical measures or liver histological stage . Over 24 months in both groups , serum bilirubin and histologic stage remained stable and alkaline phosphatase decreased significantly . For all patients , synthesis of IL-1 beta increased constitutively and in response to immune-mediated stimulants ( PHA , IL-2 , and mitochondrial antigen ) but not the bacterial stimulants LPS or S epidermidis . Compared with levels of IL-1 beta at entry , PHA induced increases for patients treated with methotrexate ( 12 , 18 , and 24 months ) or colchicine ( 18 and 24 months ) . At 24 months , IL-2-induced IL-1 beta synthesis was increased in patients treated with methotrexate , whereas S epidermidis-induced IL-1 beta was enhanced in colchicine-treated patients . Before treatment , IL-1 beta production did not relate to severity of disease except in response to S epidermidis . ( ABSTRACT TRUNCATED AT 250 WORDS )
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Intervention_Pharmacological
methotrexate
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Intervention_Pharmacological
colchicine
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Intervention_Pharmacological
Colchicine
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Intervention_Pharmacological
methotrexate
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Intervention_Pharmacological
colchicine
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Intervention_Pharmacological
methotrexate
81546
Intervention_Pharmacological
methotrexate
81547
Intervention_Pharmacological
colchicine-treated
81548
Outcome_Physical
Interleukin-1 beta ( IL-1 beta )
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Outcome_Physical
interleukin-1 ( IL-1 ) .
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Outcome_Physical
IL-1 beta
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Outcome_Physical
biochemical measures or liver histological stage .
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Outcome_Physical
serum bilirubin and histologic stage
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Outcome_Physical
alkaline phosphatase
81554
Outcome_Physical
synthesis of IL-1 beta
81555
Outcome_Physical
IL-2-induced IL-1 beta synthesis
81556
Outcome_Physical
S epidermidis-induced IL-1 beta
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Participant_Condition
primary biliary cirrhosis
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Participant_Condition
Primary biliary cirrhosis ( PBC )
81559
Participant_Condition
liver disease
81560
Participant_Sample-size
42
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Participant_Condition
PBC