83167
document
Effects of a fish-oil and vegetable-oil formula on aggregation and ethanolamine-containing lysophospholipid generation in activated human platelets and on leukotriene production in stimulated neutrophils . The effects of consuming a liquid formula containing either fish oil enriched in omega-3 fatty acids or vegetable oil enriched in oleic acid was evaluated in 20 male subjects randomly allocated into two groups over a 42-d period . A decrease in collagen-induced aggregation by using washed platelet suspensions was found in both groups after nutritional supplementation . A considerable rise in omega-3 and a decrease in omega-6 fatty acids occurred in the platelet phospholipid with fish-oil consumption . The degree of eicosapentaenoic acid ( EPA , 20:5n-3 ) enrichment ( fish-oil group ) was dramatically greater in the ether-containing plasmenylethanolamine ( 13.5 mol % of fatty acids ; mol % of fatty acids = moles per 100 moles of total fatty acids ) than in phosphatidylethanolamine ( 2.8 mol % ) or phosphatidylcholine ( 2.9 mol % ) . Neither treatment significantly influenced the agonist-induced accumulation of lysoplasmenylethanolamine as derived via phospholipase A2 hydrolysis of plasmenylethanolamine . HPLC measurements of eicosanoid production in A23187-stimulated neutrophils revealed a considerable decrease in the formation of arachidonic acid-derived leukotriene B4 ( LTB4 ) , by 41 % , and 5-HETE ( 5-hydroxyeicosatetraenoic acid ) , by 30 % , in the fish-oil group along with the appearance of the corresponding EPA-derived products [ LTB5 and 5-HEPE ( 5-hydroxyeicosapentaenoic acid ) ] . No such alterations in the formation of lipoxygenase products were found with the vegetable oil treatment .
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Intervention_Pharmacological
fish-oil and vegetable-oil formula
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Intervention_Pharmacological
liquid formula containing either fish oil enriched in omega-3 fatty acids
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Intervention_Pharmacological
vegetable oil enriched in oleic acid
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Intervention_Pharmacological
fish-oil consumption
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Intervention_Pharmacological
vegetable oil treatment
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Outcome_Physical
lysophospholipid generation
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Outcome_Physical
leukotriene production
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Outcome_Physical
collagen-induced aggregation
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Outcome_Physical
omega-3
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Outcome_Physical
omega-6 fatty acids
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Outcome_Physical
eicosapentaenoic acid
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Outcome_Physical
agonist-induced accumulation of lysoplasmenylethanolamine
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Outcome_Physical
HPLC measurements
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Outcome_Physical
eicosanoid production
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Outcome_Physical
formation of arachidonic acid-derived leukotriene B4
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Outcome_Physical
5-HETE ( 5-hydroxyeicosatetraenoic acid )
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Participant_Sample-size
20
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Participant_Sex
male