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document
In vitro biocompatibility tests of glass ionomer cements impregnated with collagen or bioactive glass to fibroblasts . AIM AND DESIGN To evaluate the biocompatibility of glass ionomer cement ( GIC ) impregnated with collagen or bioactive glass to BHK-21 fibroblasts in vitro . Mineral Trioxide Aggregate was used as the standard for comparison . Human maxillary central incisors ( n = 70 ) were instrumented with a rotary NiTi system and filled . Following resection of the apical 3mm , root end cavities were prepared and restored with conventional GIC ( group 1 ) or GIC with 0.01 % , 0.1 % or 1 % collagen ( groups 2 , 3 , 4 respectively ) or , 10 % , 30 % or 50 % bioactive glass ( groups 5 , 6 , 7 respectively ) , or Mineral Trioxide Aggregate ( group 8 ) . The root slices were incubated in tissue culture plates with BHK-21 fibroblast cell line . Phase contrast and scanning electron microscopes were used to score cell quantity , morphology and cell attachment . The data were statistically analyzed by one way ANOVA with Post Hoc Tukey HSD test ( p = 0.05 ) . RESULTS AND CONCLUSIONS Group 5 showed the highest scores which was significantly higher than all other groups ( p < 0.05 ) except group 8 , with which there was no significant difference ( p > 0.05 ) . Glass ionomer cement with 10 % bioactive glass showed better adhesion and spreading of cells than glass ionomer cement with 0.01 % collagen . The biocompatibility of collagen and bioactive glass was concentration dependent . The addition of bioactive glass improved the biocompatibility of glass ionomer cement to fibroblasts better than addition of collagen .
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Intervention_Pharmacological
conventional GIC ( group 1 ) or GIC with 0.01 % , 0.1 % or 1 % collagen ( groups 2 , 3 , 4 respectively ) or , 10 % , 30 % or 50 % bioactive glass ( groups 5 , 6 , 7 respectively ) , or Mineral Trioxide Aggregate ( group 8 ) .
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Outcome_Other
score cell quantity
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Outcome_Other
morphology
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Outcome_Other
cell attachment
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Outcome_Other
ANOVA with Post Hoc Tukey HSD test
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Outcome_Other
better adhesion
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Outcome_Other
spreading of cells
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Outcome_Other
biocompatibility of collagen and bioactive glass
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Outcome_Other
improved the biocompatibility of glass ionomer cement
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Participant_Condition
Human maxillary central incisors
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Participant_Sample-size
70