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document
Homocysteine Metabolism Gene Polymorphisms ( MTHFR C677T , MTHFR A1298C , MTR A2756G and MTRR A66G ) Jointly Elevate the Risk of Folate Deficiency . Folate deficiency is strongly associated with cardiovascular disease . We aimed to explore the joint effect of the methylenetetrahydrofolate reductase ( MTHFR ) C677T and A1298C , methionine synthase ( MTR ) A2756G , and methionine synthase reductase ( MTRR ) A66G polymorphisms on folate deficiency in a Chinese hypertensive population . A total of 480 subjects aged 28-75 were enrolled in this study from September 2005-December 2005 from six hospitals in different Chinese regions . Known genotypes were detected by PCR-RFLP methods and serum folate was measured by chemiluminescence immunoassay . Our results showed that MTHFR 677TT and MTR 2756AG + GG were independently associated with a higher risk of folate deficiency ( TT vs. CC + CT , p < 0.001 and AG + GG vs. AA p = 0.030 , respectively ) . However , the MTHFR A1298C mutation may confer protection by elevating the serum folate level ( p = 0.025 ) . Furthermore , patients carrying two or more risk genotypes showed higher odds of folate deficiency than null risk genotype carriers , especially those carrying four risk genotypes . These findings were verified by generalized multifactor dimensionality reduction ( p = 0.0107 ) and a cumulative effects model ( p = 0.001 ) . The results of this study have shown that interactions among homocysteine metabolism gene polymorphisms lead to dramatic elevations in the folate deficiency risk .
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Intervention_Pharmacological
methylenetetrahydrofolate reductase ( MTHFR ) C677T and A1298C
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Intervention_Pharmacological
methionine synthase ( MTR ) A2756G
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Intervention_Pharmacological
methionine synthase reductase ( MTRR ) A66G
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Intervention_Pharmacological
MTHFR 677TT
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Intervention_Pharmacological
MTR 2756AG + GG
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Intervention_Pharmacological
MTHFR A1298C
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Outcome_Physical
folate deficiency
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Outcome_Physical
serum folate
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Outcome_Other
MTHFR 677TT and MTR 2756AG + GG
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Outcome_Physical
folate deficiency
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Outcome_Physical
serum folate level
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Outcome_Physical
folate deficiency
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Participant_Condition
Chinese hypertensive population
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Participant_Age
A
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Participant_Sample-size
480
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Participant_Age
aged 28-75 were
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Participant_Age
.