71174
document
Differential effect of aspirin on thromboxane and prostaglandin biosynthesis in man . 1 . Effects of a single intravenous dose of aspirin ( 600 mg ) on bradykinin-stimulated prostaglandin ( PG ) and on thromboxane ( TX ) biosynthesis were determined in nine healthy male volunteers . Plasma concentrations of 6-oxo-PGF1 alpha and 13,14-dihydro-15-oxo-PGF2 alpha were measured in samples obtained during repeated 10 min intravenous infusions of bradykinin before and up to 6 h after the dose of aspirin . TXB2 was measured in serum from blood allowed to clot at 37 degrees C. 2 . Aspirin inhibited bradykinin stimulated PG and platelet TX biosynthesis 0.5 h after the dose . Serum TXB2 remained low , whereas PG synthesis recovered within 6 h. 3 . Effects of intravenous sodium salicylate ( 600 mg ) were studied identically in eight subjects . Prostanoid biosynthesis was not inhibited . 4 . Biosynthesis of prostacyclin and TXA2 under basal conditions was studied in eight subjects by measuring 2,3-dinor-6-oxo-PGF1 alpha and 2,3-dinor-TXB2 in hourly urine samples obtained during and after intravenous infusion of aspirin and , on a separate occasion , of vehicle . 5 . Aspirin infusion reduced urinary excretion of both metabolites greater than 90 % , but excretion of 2,3-dinor-6-oxo-PGF1 alpha recovered more rapidly than did that of 2,3-dinor-TXB2 . 6 . We conclude that cyclo-oxygenase is rapidly synthesised in bradykinin-responsive tissues in vivo and that this reflects similarly rapid enzyme biosynthesis in tissues that produce PGI2 under basal conditions .
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Intervention_Pharmacological
aspirin
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Intervention_Pharmacological
aspirin
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Intervention_Pharmacological
aspirin
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Intervention_Pharmacological
Aspirin
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Intervention_Pharmacological
intravenous sodium salicylate
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Intervention_Pharmacological
infusion of aspirin
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Intervention_Pharmacological
Aspirin infusion
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Outcome_Physical
thromboxane and prostaglandin biosynthesis
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Outcome_Physical
bradykinin-stimulated prostaglandin ( PG ) and on thromboxane ( TX ) biosynthesis
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Outcome_Physical
TXB2 was measured in serum
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Outcome_Physical
Aspirin inhibited bradykinin stimulated PG
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Outcome_Physical
Serum TXB2 remained low
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Outcome_Physical
PG synthesis recovered
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Outcome_Physical
Prostanoid biosynthesis
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Outcome_Physical
Biosynthesis of prostacyclin and TXA2
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Outcome_Physical
2,3-dinor-6-oxo-PGF1 alpha
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Outcome_Physical
2,3-dinor-TXB2
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Outcome_Physical
urinary excretion
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Outcome_Physical
2,3-dinor-6-oxo-PGF1 alpha recovered more rapidly
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Outcome_Physical
rapidly synthesised in bradykinin-responsive tissues
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Participant_Condition
thromboxane and prostaglandin biosynthesis
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Participant_Sex
man
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Participant_Sample-size
nine
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Participant_Condition
healthy
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Participant_Sex
male