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document
Effect of antioxidant supplementation on ozone-induced lung injury in human subjects . To determine whether antioxidants can influence human susceptibility to ozone ( O ( 3 ) ) -induced changes in lung function and airway inflammation , we placed 31 healthy nonsmoking adults ( 18 to 35 yr old ) on a diet low in ascorbate for 3 wk . At 1 wk , subjects were exposed to filtered air for 2 h while exercising ( 20 L/min/m ( 2 ) ) , and then underwent bronchoalveolar lavage ( BAL ) and were randomly assigned to receive either a placebo or 250 mg of vitamin C , 50 IU of alpha-tocopherol , and 12 oz of vegetable cocktail daily for 2 wk . Subjects were then exposed to 0.4 ppm O ( 3 ) for 2 h and underwent a second BAL . On the day of the O ( 3 ) exposure , supplemented subjects were found to have significantly increased levels of plasma ascorbate , tocopherols , and carotenoids as compared with those of the placebo group . Pulmonary function testing showed that O ( 3 ) -induced reductions in FEV ( 1 ) and FVC were 30 % and 24 % smaller , respectively , in the supplemented cohort . In contrast , the inflammatory response to O ( 3 ) inhalation , as represented by the percent neutrophils and the concentration of interleukin-6 recovered in the BAL fluid at 1 h after O ( 3 ) exposure was not different for the two groups . These data suggest that dietary antioxidants protect against O ( 3 ) -induced pulmonary function decrements in humans .
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Intervention_Pharmacological
antioxidant supplementation
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Intervention_Pharmacological
antioxidants
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Intervention_Other
diet low in ascorbate
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Intervention_Physical
filtered air for 2 h while exercising ( 20 L/min/m ( 2 ) )
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Intervention_Physical
bronchoalveolar lavage ( BAL )
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Intervention_Control
placebo
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Intervention_Pharmacological
250 mg of vitamin C
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Intervention_Pharmacological
50 IU of alpha-tocopherol
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Intervention_Pharmacological
vegetable cocktail daily for 2 wk
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Intervention_Other
BAL
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Intervention_Pharmacological
dietary antioxidants
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Outcome_Physical
lung function
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Outcome_Physical
airway inflammation
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Outcome_Physical
levels of plasma ascorbate , tocopherols , and carotenoids
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Outcome_Physical
O ( 3 ) -induced reductions in FEV ( 1 ) and FVC
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Outcome_Physical
inflammatory response to O ( 3 )
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Outcome_Physical
percent neutrophils and the concentration of interleukin-6
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Outcome_Physical
O ( 3 ) -induced pulmonary function
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Participant_Condition
ozone-induced lung injury in human subjects .
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Participant_Sample-size
31