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document
Prospective randomized trial of short-term neoadjuvant chemotherapy for advanced gastric cancer . BACKGROUND We performed short-term neoadjuvant chemotherapy ( s-NAC ) to examine whether anticancer drugs can change the proliferative ability of cancer cells in gastric cancer patients . METHODS Chemotherapy was performed for 72 h before gastrectomy in 63 gastric cancer patients . Patients were classed into four groups : Group F , 16 cases who received a single administration of 5-fluorouracil ( 5-FU ) ; Group C , 15 cases who received a single administration of cis-diamminedichloroplatinum ( CDDP ; cisplatin ) ; Group FC , 16 cases who received both 5-FU+CDDP ; and a Control group , 16 cases who did not receive chemotherapy . We reviewed neoadjuvant biopsy tissue and gastric cancer tissue delivered by operation in these cases . The TUNEL method and immunohistochemistry with an anti-MIB-1 antibody were used to evaluate cellular apoptosis and proliferative ability , respectively . The apoptotic index ( AI ) and an MIB-1 index ( MI ) were also calculated . RESULTS There were no differences in AI or MI in biopsy tissue between the groups . The AI of gastric cancer tissue in Group FC was significantly higher than in the other groups ( P < 0.01 ) . The MI of Group FC was significantly lower than in the other groups ( P < 0.05 ) . In addition , after s-NAC operation there was a significant inhibition of proliferative potency and an induction of apoptosis in Group FC . CONCLUSION Combination of CDDP and 5-FU reduced proliferative potency and increased cellular apoptosis in gastric cancer cells .
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Intervention_Physical
short-term neoadjuvant chemotherapy
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Intervention_Physical
short-term neoadjuvant chemotherapy ( s-NAC )
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Intervention_Pharmacological
5-fluorouracil ( 5-FU )
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Intervention_Pharmacological
cis-diamminedichloroplatinum ( CDDP ; cisplatin )
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Intervention_Pharmacological
5-FU+CDDP
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Intervention_Control
not receive chemotherapy
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Intervention_Pharmacological
s-NAC
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Intervention_Pharmacological
Combination of CDDP and 5-FU
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Outcome_Physical
cellular apoptosis and proliferative ability
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Outcome_Physical
apoptotic index ( AI )
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Outcome_Physical
MIB-1 index ( MI )
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Outcome_Physical
AI or MI
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Outcome_Physical
AI
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Outcome_Physical
gastric cancer tissue
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Outcome_Physical
MI of Group FC
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Outcome_Physical
proliferative potency
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Outcome_Physical
apoptosis
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Outcome_Physical
proliferative potency
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Outcome_Physical
cellular apoptosis
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Participant_Condition
advanced gastric cancer .
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Participant_Condition
gastric cancer
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Participant_Sample-size
63
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Participant_Sample-size
16
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Participant_Sample-size
15
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Participant_Sample-size
16
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Participant_Sample-size
16
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Participant_Condition
gastric cancer