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document
Efficiency of slush nitrogen vitrification of human oocytes vitrified with or without cumulus cells in relation to survival rate and meiotic spindle competence . OBJECTIVE To evaluate the efficiency of slush nitrogen vitrification of human oocytes with or without cumulus cells in terms of survival rate and maintenance of meiotic spindle . DESIGN Randomized , comparative study . SETTING Medical center . PATIENT ( S ) A total of 274 oocytes obtained from 46 couples undergoing infertility treatment . INTERVENTION ( S ) Metaphase II oocytes were divided into groups A and B , vitrified with and without cumulus cells , respectively . MAIN OUTCOMES MEASURE ( S ) Survival rates and maintenance of meiotic spindle observed immediately after warming and 3 hours after incubation . RESULT ( S ) No statistically significant difference was detected between the two groups in terms of survival rate , but a significantly higher percentage of detectable spindle was observed in group B ( completely denuded oocytes ) , either immediately after warming or 3 hours after incubation . CONCLUSION ( S ) Complete denudation of oocytes before slush nitrogen vitrification does not influence survival rates but positively affects oocyte meiotic spindle competence . These data support the hypothesis that cumulus cells during vitrification represent an obstacle to cryoprotectant penetration more than having a protective role for the oocyte .
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Intervention_Pharmacological
slush nitrogen vitrification
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Intervention_Physical
vitrified with or without cumulus cells
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Intervention_Pharmacological
slush nitrogen vitrification
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Intervention_Physical
with or without cumulus cells
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Intervention_Physical
vitrified with and without cumulus cells
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Outcome_Other
Efficiency
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Outcome_Mortality
survival rate
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Outcome_Physical
meiotic spindle competence
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Outcome_Other
efficiency
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Outcome_Physical
maintenance of meiotic spindle
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Outcome_Mortality
Survival rates
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Outcome_Other
maintenance of meiotic spindle observed immediately after warming and 3 hours after incubation
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Outcome_Mortality
survival rate
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Outcome_Physical
detectable spindle
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Outcome_Mortality
survival rates
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Outcome_Physical
meiotic spindle competence
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Participant_Sample-size
46
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Participant_Condition
infertility