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document
Effect of broccoli intake on markers related to oxidative stress and cancer risk in healthy smokers and nonsmokers . Cruciferous vegetables ( CVs ) have been widely studied for their anticarcinogenic properties . The aim of the present study was to evaluate the protective effect of broccoli intake in smokers and nonsmokers . Twenty young healthy males ( 10 smokers and 10 nonsmokers ) were randomized in a cross-over design and received a portion of broccoli ( 200 g ) or maintained a controlled diet for 10 days each . The two periods were separated by a wash-out period ( 20 days ) . Blood samples were collected at 0 , 10 , 30 , and 40 days and used for the evaluation of DNA damage , insulin-like growth factor-I ( IGF-I ) and histone deacetylase ( HDAC ) . Ex vivo protection from H ( 2 ) O ( 2 ) -induced DNA damage and endogenous DNA damage were evaluated in lymphocytes by means of the comet assay . Strand breaks decreased significantly after the broccoli diet in smokers as well as in nonsmokers ( -22.2 % ; P < 0.0001 ) , whereas oxidized purines decreased significantly only in smokers ( -51.0 % ; P < 0.0001 ) . Broccoli intake did not modify HDAC activity and IGF-I serum levels . Our results strengthen the importance of consuming CVs to increase cell protection against DNA damage . Future investigation , with different amount of broccoli and/or different time of exposure , is needed to understand the lack of effect on HDAC activity and IGF-I levels .
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Intervention_Pharmacological
broccoli intake
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Intervention_Other
broccoli
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Intervention_Control
maintained a controlled diet
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Outcome_Physical
markers related to oxidative stress and cancer risk
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Outcome_Physical
DNA damage , insulin-like growth factor-I ( IGF-I ) and histone deacetylase ( HDAC ) . Ex vivo protection from H ( 2 ) O ( 2 ) -induced DNA damage and endogenous DNA damage
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Outcome_Physical
Strand breaks
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Outcome_Physical
oxidized purines
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Outcome_Physical
HDAC activity and IGF-I serum levels .
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Outcome_Physical
increase cell protection against DNA damage .
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Outcome_Physical
HDAC activity and IGF-I levels .