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document
Studies on leptin and leptin receptor gene expression in myometrium and uterine myomas of gnRH analogue-treated women . AIM To test if treatment with GnRH analogue , which leads to a significant reduction in myoma volume , changes expression of leptin genes and gene coding leptin receptor isoforms in uterine myomas and in the surrounding unaltered myometrium . METHODS Using RT-PCR , expression of leptin genes and leptin receptor genes was studied in myomas and in the surrounding myometrium in women with uterine myomas , untreated or treated with GnRH analogue . In the randomly selected cases presence of leptin protein and of leptin receptor proteins was examined also by Western blotting . RESULTS Expression of leptin genes was demonstrated both in myomas and in the surrounding myometrium , and a similar pattern of expression was found for leptin receptor isoforms . The results of RT-PCR were confirmed by Western blotting , which documented the identical distribution of leptin proteins and leptin receptor proteins in studied tissues . Treatment with GnRH analogue had no effect on the expression pattern of studied genes . CONCLUSION The results of the present study on the administration of GnRH analogue to females with myomas suggest that no direct or immediate inter-relationship exists between expression of leptin genes in uterine myomas on one hand and estrogen , progesterone and leptin levels in the blood on the other . Expression seems to be of a more durable nature but factors that induce such expression remain unknown .
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Intervention_Physical
gnRH analogue-treated
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Intervention_Physical
GnRH analogue
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Intervention_Pharmacological
GnRH analogue
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Intervention_Physical
GnRH analogue
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Intervention_Physical
GnRH analogue
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Outcome_Physical
leptin and leptin receptor gene expression
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Outcome_Physical
myoma volume
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Outcome_Physical
leptin receptor isoforms
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Outcome_Physical
Expression of leptin genes
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Outcome_Physical
leptin proteins and leptin receptor proteins
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Outcome_Physical
expression pattern
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Outcome_Physical
leptin genes
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Participant_Sex
women
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Participant_Condition
uterine myomas
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Participant_Condition
untreated or treated with GnRH analogue