47945
document
Gene expression of endothelin-1 and its receptors in the heart of broiler chickens with T ( 3 ) -induced pulmonary hypertension . To investigate the effect of T ( 3 ) -induced pulmonary hypertension on endothelin ( ET ) production and genes expression of ET-1 , ET ( A ) and ET ( B ) receptors ( ET ( A ) R and ET ( B ) R ) during rearing , semiquantitative RT-PCR and enzyme immunometric assay were performed in the heart ventricles and serum , respectively . The ET-1 and its receptor genes were expressed in the right and left ventricles of control and T ( 3 ) -treated broilers at 12 , 28 and 49 days of age . There were significant ( P < 0.05 ) reductions of the relative amounts of ET-1 ( in both ventricles ) and ET ( A ) R ( in the right ventricle ) mRNAs at 28 and 49 days of age , in T ( 3 ) -treated broilers compared to controls . The relative amounts of ET ( B ) R mRNA in the right and left ventricles did not significantly differ between control and T ( 3 ) -treated broilers at any age . The serum level of ET was significantly ( P < 0.05 ) increased in T ( 3 ) -treated chickens at 28 and 49 days of age when compared with that of the control . It is concluded that ET-1 , ET ( A ) R and ET ( B ) R genes are normally expressed in the heart ventricles of broilers . It is likely that increased serum level of ET and decreased ET-1/ET ( A ) R genes expression in the ventricles are involved in the heart dysfunction of broiler chickens with developmental pulmonary hypertension .
47946
Intervention_Other
semiquantitative RT-PCR
47947
Intervention_Other
enzyme immunometric assay
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Outcome_Physical
genes expression of ET-1 , ET ( A ) and ET ( B ) receptors ( ET ( A ) R and ET ( B ) R )
47949
Outcome_Physical
ET-1 and its receptor genes
47950
Outcome_Physical
reductions of the relative amounts of ET-1 ( in both ventricles ) and ET ( A ) R ( in the right ventricle ) mRNAs
47951
Outcome_Physical
relative amounts of ET ( B ) R mRNA
47952
Outcome_Physical
serum level of ET
47953
Outcome_Physical
ET-1 , ET ( A ) R and ET ( B ) R genes
47954
Outcome_Physical
serum level of ET and decreased ET-1/ET ( A ) R genes expression
47955
Participant_Condition
heart of broiler chickens with T ( 3 ) -induced pulmonary hypertension
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Participant_Condition
broiler chickens with developmental pulmonary hypertension