31887
document
Protein ingestion further augments S6K1 phosphorylation in skeletal muscle following resistance type exercise in males . Our objective was to determine the impact of carbohydrate and/or protein ingestion before and after exercise on ribosomal protein S6 kinase ( S6K1 ) and S6 phosphorylation status in human skeletal muscle tissue . Seven healthy , untrained men ( 22.5 +/- 0.9 y ) were randomly assigned to 2 cross-over experiments . Before , immediately after , and 1 h after a single bout of resistance exercise , subjects consumed 0.3 g x kg ( -1 ) carbohydrate with or without 0.3 g x kg ( -1 ) protein hydrolysate ( CHO+PRO and CHO , respectively ) . Muscle biopsies were taken before and immediately after exercise and after 1 and 4 h of postexercise recovery to determine 4E-BP1 , S6K1 ( both T ( 421 ) /S ( 424 ) and T ( 389 ) ) , and S6 phosphorylation status . Following resistance exercise , 4E-BP1 phosphorylation was reduced to a greater extent in the CHO treatment ( -48 +/- 7 % ) than in the CHO+PRO treatment ( -15 +/- 14 % , P < 0.01 ) . During recovery , 4E-BP1 phosphorylation increased in both experiments ( P < 0.01 ) , and tended to be higher in the CHO+PRO test ( P = 0.08 ) . S6K1 phosphorylation at T ( 421 ) /S ( 424 ) substantially increased following exercise and remained elevated during recovery with no differences between treatments . In contrast to the CHO treatment ( -4 +/- 2 % ) , S6K1 phosphorylation at T ( 389 ) was higher following exercise in the CHO+PRO treatment only ( +78 +/- 2 % , P < 0.01 ) . During recovery , S6K1 phosphorylation at T ( 389 ) remained higher in CHO+PRO than in CHO ( P < 0.05 ) . S6 phosphorylation was substantially higher following exercise in the CHO+PRO ( 1.69 +/- 0.35 ) than in the CHO experiment ( 0.45 +/- 0.07 , P < 0.01 ) and remained elevated during recovery ( P < 0.05 ) . We conclude that the availability of dietary protein further enhances phosphorylation of S6K1 during recovery from resistance type exercise .
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Intervention_Pharmacological
Protein ingestion
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Intervention_Control
carbohydrate
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Intervention_Pharmacological
protein
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Intervention_Physical
exercise
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Intervention_Physical
resistance exercise
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Intervention_Control
carbohydrate
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Intervention_Pharmacological
protein hydrolysate
31895
Intervention_Pharmacological
CHO+PRO
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Intervention_Control
CHO
31897
Intervention_Physical
Muscle biopsies
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Intervention_Pharmacological
4E-BP1 phosphorylation
31899
Intervention_Control
CHO
31900
Intervention_Pharmacological
CHO+PRO
31901
Intervention_Pharmacological
CHO
31902
Intervention_Pharmacological
CHO+PRO
31903
Intervention_Pharmacological
CHO+PRO
31904
Intervention_Pharmacological
CHO
31905
Intervention_Pharmacological
CHO+PRO
31906
Intervention_Pharmacological
CHO
31907
Outcome_Physical
4E-BP1 phosphorylation
31908
Outcome_Physical
4E-BP1 phosphorylation
31909
Outcome_Physical
S6K1 phosphorylation at T ( 421 ) /S ( 424 )
31910
Outcome_Other
S6K1 phosphorylation at T ( 389 )
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Outcome_Physical
During recovery , S6K1 phosphorylation at T ( 389 )
31912
Outcome_Physical
S6 phosphorylation
31913
Participant_Sex
males
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Participant_Sample-size
Seven
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Participant_Sex
men
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Participant_Age
( 22.5 +/- 0.9 y )