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document
[ Effects of dujieqing oral liquid on the promoter methylation of the MGMT gene in middle-and-late stage tumor patients receiving chemotherapy ] . OBJECTIVE To observe the effects of Dujieqing Oral Liquid ( DJQ ) on the promoter methylation of the O6-methylguanine-DNA methyltransferase ( MGMT ) gene in the plasma DNA samples from middle-and-late stage tumor patients receiving chemotherapy . METHODS Recruited 60 patients were randomly assigned to the treatment group ( treated by conventional chemotherapy combined DJQ , 20 mL each time , three times daily ) and the control group ( treated by chemotherapy alone ) , 30 in each group . The therapeutic course was 8 weeks . The promoter methylation of the MGMT gene in the plasma DNA samples form middle-and-late stage tumor patients receiving chemotherapy was detected before and after treatment using nested methylation-specific polymerase chain reaction ( MSP ) . Meanwhile , the peripheral hemogram was detected . The clinical efficacy and toxic/adverse reactions were assessed using Karnofsky performance scale ( KPS ) . RESULTS Results of the promoter methylation of MGMT genes showed that methylation rate was 20.00 % in the treatment group and 46.67 % in the control group ( P < 0.05 ) . Compared with before treatment , the KPS was significantly improved in the treatment group after treatment , while it significantly decreased in the control group after treatment ( both P < 0.05 ) . There was statistical difference in the KPS between the two groups after treatment ( P < 0.01 ) . The toxic/adverse reactions were milder in the treatment group than in the control group ( P < 0.01 ) . CONCLUSIONS DJQ showed efficiency synergism and toxicity reducing effects , but with no effect on the hematopoietic function of the bone marrow . MGMT gene was indicated as DJQ 's target point for efficiency synergism and toxicity reducing . The efficiency synergism and toxicity reducing effects were achieved by regulating the activities of MGMT gene .
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Intervention_Pharmacological
dujieqing oral liquid
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Intervention_Pharmacological
Dujieqing Oral Liquid ( DJQ )
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Intervention_Pharmacological
conventional chemotherapy combined DJQ
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Intervention_Control
control group ( treated by chemotherapy alone )
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Intervention_Pharmacological
DJQ
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Intervention_Pharmacological
DJQ 's
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Outcome_Physical
clinical efficacy
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Outcome_Adverse-effects
toxic/adverse reactions
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Outcome_Physical
KPS
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Outcome_Physical
KPS
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Outcome_Adverse-effects
toxic/adverse reactions
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Participant_Condition
middle-and-late stage tumor
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Participant_Condition
receiving chemotherapy
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Participant_Condition
middle-and-late stage tumor
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Participant_Sample-size
60
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Participant_Condition
middle-and-late stage tumor