86583
document
No effect of beta-carotene supplementation on the susceptibility of low density lipoprotein to in vitro oxidation among hypercholesterolaemic , postmenopausal women . The effect of beta-carotene on the susceptibility of low density lipoprotein ( LDL ) to oxidative modification was investigated in a double-blind , randomized placebo-controlled study . Hypercholesterolaemic , postmenopausal women were given 30 mg beta-carotene per day ( n = 15 subjects ) or placebo capsules ( n = 15 subjects ) for 10 weeks . They were instructed to follow the American Heart Association Step One diet . LDL , isolated before and after treatment was subjected to copper-catalysed lipid peroxidation . There were no significant differences between LDL from the beta-carotene and placebo groups , as assessed by measuring the lag time for formation of conjugated dienes ; the rate of formation and the amount of conjugated dienes formed ; the amount of lipid peroxides generated ; and the relative electrophoretic mobility , at baseline and after treatment . Dietary records showed that the subjects were consuming similar amounts and types of fat . No significant differences were found in the lipid composition and fatty acid pattern of LDL from the two groups . In conclusion , the results indicated that supplementation with beta-carotene in non-smoking , hypercholesterolaemic , postmenopausal women had no protective effect on the susceptibility of LDL to copper-catalysed modification in vitro .
86584
Intervention_Pharmacological
beta-carotene supplementation
86585
Intervention_Pharmacological
beta-carotene
86586
Intervention_Pharmacological
30 mg beta-carotene per day
86587
Intervention_Control
placebo capsules
86588
Intervention_Pharmacological
beta-carotene
86589
Intervention_Control
placebo
86590
Intervention_Pharmacological
beta-carotene
86591
Outcome_Physical
in vitro oxidation
86592
Outcome_Physical
LDL
86593
Outcome_Physical
lag time for formation of conjugated dienes
86594
Outcome_Physical
rate of formation
86595
Outcome_Physical
the amount of conjugated dienes formed
86596
Outcome_Physical
amount of lipid peroxides generated
86597
Outcome_Physical
electrophoretic mobility
86598
Outcome_Physical
lipid composition and fatty acid pattern of LDL
86599
Outcome_Physical
protective effect
86600
Participant_Condition
hypercholesterolaemic
86601
Participant_Condition
postmenopausal
86602
Participant_Sex
women
86603
Participant_Condition
Hypercholesterolaemic
86604
Participant_Condition
postmenopausal women
86605
Participant_Condition
non-smoking
86606
Participant_Condition
hypercholesterolaemic
86607
Participant_Condition
postmenopausal women