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document
Examining the temperature of embryo culture in in vitro fertilization : a randomized controlled trial comparing traditional core temperature ( 37°C ) to a more physiologic , cooler temperature ( 36°C ) . OBJECTIVE To determine whether culture at a more physiologically cooler temperature , as suggested by limited human and animal data , would improve blastulation and pregnancy rates in human clinical IVF . DESIGN Paired randomized controlled trial . SETTING Academic . PATIENT ( S ) Infertile couples ( n=52 ) with a female partner less than 42 years old with eight or more mature oocytes retrieved . INTERVENTION ( S ) Mature oocytes obtained from a single cohort of oocytes were randomly divided into two groups ; one was cultured at 37°C and the other at 36°C from the time of ICSI to the time of embryo transfer or vitrification . Paired embryo transfers were accomplished by transferring one euploid embryo from each group . DNA fingerprinting was used as needed to determine the outcome for each embryo . MAIN OUTCOME MEASURE ( S ) Rate of development of expanded blastocysts suitable for transfer or vitrification ( primary outcome ) , fertilization , aneuploidy , and sustained implantation . RESULT ( S ) A total of 805 mature oocytes were cultured ; 399 at 36°C and 406 at 37°C . Paired analysis demonstrated a higher rate of usable blastocyst formation per zygote at 37°C ( 48.4 % ) vs. at 36°C ( 41.2 % ) . Rates of fertilization , aneuploidy , and sustained implantation were equivalent . CONCLUSION ( S ) IVF culture at 36°C does not improve clinically relevant parameters of embryo development or sustained implantation rates . CLINICAL TRIAL REGISTRATION NUMBER NCT01506089 .
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Intervention_Other
cultured at 37°C
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Intervention_Other
36°C
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Outcome_Physical
Rate of development of expanded blastocysts suitable for transfer or vitrification ( primary outcome )
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Outcome_Physical
fertilization , aneuploidy , and sustained implantation
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Outcome_Physical
rate of usable blastocyst formation per zygote
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Outcome_Physical
Rates of fertilization , aneuploidy , and sustained implantation
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Participant_Condition
in vitro fertilization :