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[Effect of SP on intracellular free calcium concentration in cultured osteoblast].

OBJECTIVE
To investigate the effect of SP on intracellular free calcium concentration (Ca2+)i in cultured osteoblast.


METHODS
Effect of SP on intracellular free calcium was measured using cell culturing and laser scanning confocal microscopy with fluo-3/AM as a fluorescence probe.


RESULTS
After treated with SP(10(-4) mol/L), 70% of the cells responded to it; the intracellular (Ca2+)i increased markedly [fluorescence intensity increased by (90 +/- 18)%].


CONCLUSIONS
These results suggested that calcium may have a role as a intracellular secondary messenger in the action of SP in osteoblast.