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Quantitation of selective autophagic protein aggregate degradation in vitro and in vivo using luciferase reporters

In preparation to perform a high throughput screen with the vectors described in Quantitation of selective autophagic protein aggregate degradation in vitro and in vivo using luciferase reporters (Autophagy 2009; 5: 511–9), we discovered an unintended missense mutation resulting in a TAG amber stop codon between polyglutamine-19/80 and luciferase. Translational readthrough at this stop codon results in a ~10 fold reduction in full length polyQ-luciferase expression as compared to a full length luciferase construct or the corrected polyQ19/80-luciferase construct. We have re-confirmed that polyQ80-luciferase is selectively degraded upon autophagic stimuli when compared with a polyQ19-luciferase expression construct and the conclusions of the original paper remain unchanged. We regret any confusion this may have caused other investigators. Quantitation of selective autophagic protein aggregate degradation in vitro and in vivo using luciferase reporters