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Spectrophotometric methods for the determination of satranidazole

Three new, simple and sensitive spectrophotometric methods I, II and III have been developed for the quantitative estimation of satranidazole by first reducing it with zinc granules and hydrochloric acid in ethanol followed by its reaction in situ with p-methylbenzaldehyde (Method-I) and salicylaldehyde (Method-II) in ethanol to form yellow chromogens with λ m a x 390 and 425 nm, which obeyed Beer's law in the concentration range 20-100 and 10-50 μg/ml, respectively. Method-III is based on the reaction of diazotization of the reduced satranidazole and its subsequent coupling with N-(1-naphthyl)ethylenediamine dihydrochloride at room temperature to form a pink coloured chromogen exhibiting λ m a x at 508 nm which obeyed Beer's law in the concentration range 10-50 μg/ml. These methods have been found to be applicable for quantitative estimation of satranidazole in bulk drugs and dosage formulations.