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Differential elevation of HSP72 in mouse tissues following heat exposure

We investigated heat‐induced changes in HSP72 in various organs of mice in relation with core temperature (Tc) and cardiovascular activities. Real‐time Tc, blood pressure (BP) and heart rate (HR) were examined in conscious mice during heat exposure using telemetry. Adult male mice (n=8) were exposed to heat (39.5°C) twice (7 days interval) in an environmental chamber for up to 3 hrs including 1 hr chamber heating. Tissue samples were collected 18–22 hrs following the second heat exposure. HSP72 measured by ELISA increased significantly in the gastrocnemius muscle (0.998±0.195 versus control 0.074±0.017 ng/mg dry weight, n=5, p=0.004) and liver (0.532±0.089 versus control 0.207±0.053 ng/mg dry weight, p=0.038), but not in the heart and kidneys. Plasma HSP72 in control and heat‐treated mice was below the detection limit. HSP72 levels in the gastrocnemius muscle (R2 = 0.71) and liver (R2 = 0.54) both had positive correlation with peak average HR, but not with peak Tc or peak BP during heat exposure. These results suggest that short‐term heat exposure may selectively target some organs to cause up‐regulation of HSP72. In vivo HSP72 synthesis induced by heat is a complex stress response. (Supported by USUHS Grant R091EH)