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Protein Lipidation Types: Current Strategies for Enrichment and Characterization

Post-translational modifications regulate diverse activities of a colossal number of proteins. For example, various types of lipids can be covalently linked to proteins enzymatically or non-enzymatically. Protein lipidation is perhaps not as extensively studied as protein phosphorylation, ubiquitination, or glycosylation although it is no less significant than these modifications. Evidence suggests that proteins can be attached by at least seven types of lipids, including fatty acids, lipoic acids, isoprenoids, sterols, phospholipids, glycosylphosphatidylinositol anchors, and lipid-derived electrophiles. In this review, we summarize types of protein lipidation and methods used for their detection, with an emphasis on the conjugation of proteins with polyunsaturated fatty acids (PUFAs). We discuss possible reasons for the scarcity of reports on PUFA-modified proteins, limitations in current methodology, and potential approaches in detecting PUFA modifications.


Introduction
Proteins play indispensable roles in maintaining cell survival, and their functions are often regulated by post-translational modifications (PTMs), in which proteins are proteolytically cleaved or enzymatically conjugated with modifying groups. Various enzymes, including kinases, phosphatases, transferases, and ligases, catalyze approximately 500 discrete PTMs of a diverse set of proteins [1,2]. PTM of proteins occurs at all stages of human life, and abnormal PTM often leads to various diseases [3][4][5][6][7].
Well-studied PTMs include protein glycosylation, methylation, hydroxylation, amidation, phosphorylation, acetylation, and ubiquitination [2,8]. Protein lipidation is perhaps not as extensively studied as protein phosphorylation or acetylation even though it is no less significant than other modifications [9][10][11][12][13]. Various lipids or lipid metabolites can be covalently attached to proteins, and this PTM is accordingly called under different names, including protein lipidation [7], fatty acylation [14], and lipid modifications of proteins [15]. Nearly 20% of all proteins are known to be lipid modified [16], which is relatively rare, resulting in the detection difficulty and need of enrichment techniques for characterization.
There exist many technologies to characterize lipidated proteins by taking full advantage of the proteins' characteristics, including spectroscopic methods, such as nuclear magnetic resonance (NMR) spectroscopy [17] and circular dichroism (CD) spectroscopy [18] (according to membrane protein structure and dynamics), crystallography [19,20] (according to lipidated protein dimensional structure), mass spectrometry (MS) [7] (according to lipidated protein fragment-ion characteristics), and so on. However, given the wide structural variability of lipid moieties of lipidated proteins, highly sensitive and specific methods for its detection are required [21]. Adequate enrichment followed by MS will be a more effective strategy.
There are many related reviews about protein lipidation, however, these reviews focused on the most common lipid-modifications, such as S-palmitoylation. For instance, Xu et al. reviewed S-palmitoylation and its significance in protein regulation, cell signaling, and diseases [7]. Here, we summarize the various types of protein lipidation, with an emphasis on polyunsaturated fatty acid (PUFA) modification, and methods used to detect them.

Types of Protein Lipidation
To date, studies have shown that proteins can be modified by at least seven types of lipids, including fatty acids, lipoic acids, isoprenoids, sterols, phospholipids, glycosylphosphatidylinositol (GPI) anchors, and lipid-derived electrophiles (LDEs).

Carbonyls
Nucleophilic residues [72,73] Aldehydes N-system nomenclature was used for the fatty acids (the order of carbon atoms starts from the methyl carbon of the fatty acid).

N-palmitoylation
N-palmitoylation is classified into N-terminal palmitoylation and N ε -palmitoylation according to the position of the modification in the protein (Table 1). In N-terminal palmitoylation, palmitic acid is linked to the amino group of the cysteine residue at the N-terminus of substrate proteins, whereas in N ε -palmitoylation, palmitic acid is covalently attached to the ε-amino group of the lysine residue at the N-terminus via an amide bond. The biological significance of N-terminal palmitoylation has been reviewed before [31,44,74]. A unique dual palmitoylation in the N-terminal region of the human LIM domain kinase 1 (LIMK1) controls the targeting of this protein to the spine and contributes to the activation of the protein by membrane-localized p21-activated kinase (PAK) [74]. N-terminal palmitoylation has also been detected in Sonic Hedgehog (SHh) proteins [75] and shown to be catalyzed by Hedgehog acyltransferase (HHAT) [76]. Additionally, Sirtuin (SIRT) has been reported to be modified by N ε -palmitoylation [45,46,77,78].

O-palmitoylation
Palmitic acid can be irreversibly linked to the side chain of serine residues in proteins via ester bonds in organisms without specific enzymes removing the attached lipid chain (Table 1). Currently, only a few proteins are known to be O-palmitoylated. One of them is histone H4, which can be O-palmitoylated at Ser-45 by an enzyme called lysophosphatidylcholine acyltransfer ase 1 (LPCAT1) [47]. Interestingly, O-palmitoylation at the threonine residue in the C-terminal of the spider venom neurotoxin PLTX-II has been reported, and it is thought to regulate the toxin activity in blocking presynaptic voltage-gated Ca 2+ channels [48].
2.1.6. Acylation of Unsaturated Fatty Acids Physeterylation (C14:1n9) is detected in the retina, heart, and liver [86] and on SRC family kinases [87]. Myristoleoyted (C14:1n5) proteins have also been found [88,89]. WNT proteins are O-palmitoleoylated with palmitoleic acid (C16:1n7) on their conserved serine residue by the O-acyltransferase Porcupine [57,58,90], and the palmitoleic acid of an Opalmitoleoylated WNT protein is removed by Notum [59]. Oleic acid (C18:1n9) modification has been reported on the lysine residue of the lens integral membrane protein aquaporin-0 and plays an important role in targeting the substrate protein to membrane domains in the bovine and human lens [60] (Table 1).

S-prenylation
S-prenylation is the attachment of isoprenoids to a cysteine residue in proteins [65]. Up to 2% of the total cellular proteins in mammalian cells are prenylated [93]. This modification occurs on one or more sidechains of a cysteine residue located at or near the C-terminus of the protein substrate. Most S-prenylated proteins contain a CAAX motif at their C-terminus, where the As are aliphatic amino acids and the X can be any amino acid [64]. Based on the properties of the X residues, S-prenylation is categorized into two major types. If the X is a leucine or any other small residue (alanine/serine/methionine), a 20-carbon geranylgeranyl group is attached to the C-terminus of the protein substrate (i.e., S-geranylgeranylation). Otherwise, a 15-carbon farnesyl isoprenoid lipid is attached (i.e., S-farnesylation) [65]. The enzyme that catalyzes protein S-farnesylation is called farnesyltransferase (FTase), whereas S-geranylgeranylation is catalyzed by geranylgeranyltransferase type I (GGTase-I) ( Table 1) or GGTase-II (also known as RabGGTase due to its specificity for Rab proteins) [90]. Inhibitors of FTase and GGTase-I are used to target Ras prenylation, especially for KRas proto-oncogene, GTPase (KRAS), which is frequently mutated in many types of cancers [94,95].

C-terminal Phosphatidylethanolaminylation
C-terminal phosphatidylethanolaminylation is the attachment of phosphatidylethanolamine (PE) to the amino group of a C-terminal glycine (Table 1). Microtubule-associated protein 1 light chain 3 alpha (LC3), a well-known protein associated with autophagy, is phosphatidylethanolaminylated [66,67].

C-terminal Cholesterolyation
C-terminal cholesterolyation is observed in Hedgehog (HH) family proteins and refers to the conjugation of cholesterol to the C-terminus of these proteins via an esterified linkage with the hydroxyl moiety of the cholesterol through an autocatalytic reaction ( Table 1). The HH family plays fundamental roles in long-range embryonic signal transduction pathways [96]. HH proteins can undergo two types of modification, namely C-terminal cholesterolyation and N-terminal palmitoylation, which are both critical for the activities of HH proteins [68,69,97].

Detection of Protein Lipidation
Detection of lipidated proteins involves challenging steps, including enrichment to identify the modification type and site, stoichiometric quantitation of the modification, and visualization of the modified protein. Despite these limitations, significant progress in the characterization of lipidated proteins has been made in the past few years.
The common "bottom up" high-throughput proteomics is considered a suitable approach to address these challenges through enrichment and digestion, multi-dimensional chromatographic separation, and high-throughput mass spectrometry detection [108][109][110][111]. Although MS-based detection approaches are highly sensitive in identifying lipidated proteins and modification sites, these approaches often require specialized protein enrichment methods, where is a filed hard to break through. Especially, for some very hydrophobic lipidated proteins, the common "bottom up" proteomics tends to underrepresent them. Thus, some studies have focused on detecting hydrophobic proteins with specific MS technique. Among them, the group of Robinson [112], who created a new technology-gas-phase structural biology MS to study hydrophobic proteins and protein-lipid interactions [113], while still deficient for high-throughput detection of lipidated proteins [114].

Radioactive Isotope-Labeling
Traditionally, metabolic incorporation of radiolabeled lipids is used to identify protein fatty acylation and prenylation [26,[115][116][117]. For instance, incorporation of radioisotope-labeled palmitic acid is used as the gold standard for identifying S-palmitoylation [25,26,117,118]. In this strategy, 3 H/ 14 C-labeled palmitic acid is added into the cell culture. The palmitic acid is then metabolically converted to palmitoyl-CoA, which attaches to a cysteine residue on substrate proteins via a thioester bond. The S-palmitoylated proteins are then detected via western blot (WB) followed autoradiography ( Figure 1A). This method does not alter the structure of fatty acid moieties. However, it is time-consuming, relatively low in sensitivity, unsuitable for high-throughput screening, and poses safety and environmental risks [119] ( Table 2).

Antibody Affinity Enrichment
A few studies have used fatty-acyl-specific antibodies to analyze lipidated proteins. In these studies, modified proteins were affinity-purified and then identified through WB or MS ( Figure 1B). Palmitoylated transitional endoplasmic reticulum ATPase [120] was identified using a pan anti-palmitoyl antibody, but this antibody has not been used in any other study yet. Using an anti-lysine 2-hydroxyisobutyrylation (Khib) antibody, 2-hydroxyisobutyrylated histone [121] was identified, and a commercial antibody of the same nature was used in later studies [122][123][124]. Although antibody-based approaches enable easy and convenient enrichment of the targeted modified proteins, pan antibodies that recognize specific lipidated proteins are difficult to generate ( Table 2).

Acyl-Biotin Exchange (ABE)
ABE was proposed in 2004 by the Drisdel group [125] to exclusively detect S-acylation of cysteine residues. This method is based on the high sensitivity of thioester bonds to weak bases such as hydroxylamine (NH 2 OH). In this method, free thiols on the cysteine residues of proteins are first blocked with N-ethylmaleimide (NEM). Next, the thioester bonds of S-palmitoylated cysteine residues are broken using NH 2 OH, and then the newly exposed thiols are captured with the biotinylated probe biotin-N-[6-(biotinamido)hexyl]-3 -(2 -pyridyldithio) propionamide (Biotin-HPDP). Afterward, S-acylated proteins are purified using streptavidin-conjugated agarose beads and identified using WB or proteins digested into peptides are subjected to LC-MS ( Figure 1C). Using this approach, hundreds of Spalmitoylated proteins have been identified [13,35,126].
In the case of the acyl-resin-assisted capture (acyl-RAC) method, the biotinylated probe is replaced with a thiopropyl sepharose resin [127] (Figure 1D). This effective strategy is more convenient than ABE.
Acyl-PEG exchange (APE) or acyl-PEGyl exchange gel shift (APEGS) is a masstag-labeling method to stoichiometrically evaluate endogenous levels of S-acylated proteins [128]. After liberating acylated cysteines by using NH 2 OH, free thiols are tagged with PEG-N-ethylmaleimide to increase the mass of each S-palmitoylated protein by adding a pre-defined PEG linker, whereby S-palmitoylated proteins can be distinguished from non-acylated proteins ( Figure 1E). The shift in mass (e.g., 5 or 10 kD) is easily detectable via SDS-PAGE/WB without further enrichment. Furthermore, researchers can easily determine the number of S-acylated sites or quantify the ratio of unmodified proteins to S-acylated proteins. The APE method, however, is difficult to scale up for high-throughput analyses.
All the three acyl-exchange methods mentioned above require complete blockage of the reduced cysteine residues, efficient thioester hydrolysis, and thorough disulfideexchange reactions to label and identify palmitoylated proteins. Furthermore, streptavidinbead enrichment is associated with a high background signal. All these factors have resulted in significant numbers of false positives [129,130]. In addition, they cannot be generalized to detect other lipid modifications, such as isoprenylation ( Table 2).

Click Chemistry
Bio-orthogonal chemical probes include terminal alkyne or azido (ω-alkyne or ωazido) lipid derivatives (fatty acids, sterols, and isoprenoids). The "click chemistry reaction" involves such probes and a highly efficient copper(I)-catalyzed cycloaddition reaction [131]. In this method, alkynyl-lipids are first metabolically incorporated. Next, the alkyne tag on the modified proteins is covalently attached to biotin-azide or a derivative through the click reaction. Subsequently, streptavidin beads are employed to pull down the proteins tagged with alkynyl-azide, and then these affinity-purified proteins digest into peptides are subjected to LC-MS to identify them and their modified sites ( Figure 1F). In contrast to ABE, bio-orthogonal labeling in conjunction with the traditional pulse-chase method allows dynamic measurement of the rates of protein incorporation and turnover. Both alkyneand azido-fatty acid probes have been developed for the click chemistry [132] and widely applied to the global analysis of N-myristoylated [133,134], S-palmitoylated [135,136], S-LDE-acylated [137,138], S-prenylated [15], cholesterolated [139], or monounsaturatedfatty-acid-modified [140] proteins.

Biotin Hydrazide Affinity Capture
Carbonyl groups, as the feature groups of various proteins modified by LDEs, can react with hydrazides to form hydrazones and be promptly reduced by borohydride to generate stable secondary amines [146]. A biotin hydrazide affinity labeling and capture approach has been deployed to enrich and analyze HNE-adducted proteins [147] (Figure 1G). It is still unclear whether the carbonyl groups are generated by LDE-modification or protein oxidation in general. Moreover, this method also detects other carbonyl modifications as background signal (Table 2).

Lipid Esterification
Lipid esterification methods mainly identify the lipid moieties in modified proteins through esterification of hydrolytically released lipid molecules, followed by gas chromatography-mass spectrometry (GC-MS) analysis. The integrated stable isotope-coded fatty acid transmethylation and mass spectrometry (iFAT-MS) method was developed to identify S-or O-acylated proteins [148]. In this method, proteins are extracted, quantified, and then resolved via SDS-PAGE. Subsequently, the gels are stained, and the protein bands are excised. The control and sample are transmethylated with d0-and d3-methanol, respectively. Derivatized fatty acids are analyzed using GC-MS ( Figure 1H). iFAT-MS is an efficient approach to distinguish between N-, S-, and O-linked fatty acyl groups. Sand O-linkages, but not N-linkages, are cleaved via alkaline-catalyzed transmethylation. NH 2 OH treatment can then differentiate between the labile S-fatty acylation and resistant O-fatty acylation. Due to the relatively low efficiency of transesterification during the NH 2 OH treatment and poor separation of the esterified acyl moieties on GC, an alternative method, which replaces NH 2 OH with platinum (IV) oxide, has been devised [149] (Table 2).
Prenylated proteins can be examined via a similar approach, in which the double bonds on prenyl groups are first reduced through hydrogenation catalyed by platinum (IV) oxide, and then the prenyl moieties are released by Raney nickel cleavage. The reduced farnesyl and geranylgeranyl groups that are released are detected as 2,6,10-trimethyldodecane and 2,6,10,14-tetramethylhexadecane, respectively [150].

Quantitative Proteomics Methods
Using the above discussed MS approaches and tag-enrichment strategies, various lipidated proteins from a wide range of organisms have been identified. However, these datasets often contain a large number of false positives. By using quantitative chemical proteomics, lipidated proteins can be quantified with high-confidence, based on signal-tonoise ratio (SNR), spectral counting, signal intensity, and qualify P-value or false discovery rates (FDR) value [36,79,134,155,156].

Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC)
In SILAC, cells are grown in media lacking certain essential amino acids but supplemented with isotopically labeled or unlabeled ones. Proteins from the test and control samples are then equally mixed and subjected to MS to quantify and identify the peptides with labeled or unlabeled amino acids [157]. Using SILAC and 17-octadecynoic acid (17-ODYA) bio-orthogonal labeling, 415 high-confidence palmitoylated proteins have been identified, and by including a pulse-chase method, a global quantitative map of dynamic protein palmitoylation events has been generated [36]. Using a combination of ABE and Stable Isotope Labeling of Mammals (SILAM), the S-palmitoylated protein profile of the glial cells from a mouse model of Huntington's disease has been characterized [158], and 151 high-confidence differentially palmitoylated proteins have been identified using the cysteine-stable isotope labeling (Cysteine-SILAC) method [159]. In this method, mass tags are incorporated to cysteine residues, and discriminated in MS with pairs (if two tags are used) or even triplets (if three). Hence the co-elution feature of the peptide isotope pairs improves confidence in their identification.

In Vitro Isotope Labeling
To profile the intrinsic reactivity of cysteine residues quantitatively, an approach named "isotopic tandem orthogonal proteolysis-activity-based protein profiling" (isoTOP-ABPP) has been described [160]. In this approach, an electrophilic alkynylated iodoacetamide (IA) probe is used to tag the cysteine residues in native proteins. The alkynyl group in IA conjugates the probe via the click chemistry to an azide-functionalized, isotopically labeled TEV-protease recognition peptide containing a biotin group. Finally, the tagged proteins are purified using streptavidin-conjugated beads and then quantified via MS. Although the isoTOP-ABPP method has been designed to quantitate reactive cysteines, it can be adapted to quantitate lipidated proteins. For instance, LDE modification of cysteines was evaluated using this approach [103]. Isobaric tagging for relative and absolute quantification/isobaric tandem mass tags (iTRAQ/TMT) [125,161] and iodoacetyl isobaric tandem mass tags (iodoTMT) [162] have also been reported.

Dynamic Visualization Methods
In addition to direct quantification, an azido fluorescence tag can be attached to specific lipidated proteins to achieve in-gel visualization by using the click chemistry approach [133]. To date, various subcellular localizations of lipidated proteins have been observed using ω-alk fatty acids [132]. Live-cell imaging of S-palmitoylated proteins has been achieved by using this bio-orthogonal strategy [163]. Additionally, imaging of global prenylated proteins by using fluorescent analogues of farnesyl and geranylgeranyl pyrophosphates has been reported [164]. In situ proximity ligation assay (PLA) alongside fluorescent imaging based on alkynyl fatty acids has been applied to track lipidated proteins with high spatial resolution in live cells [163,165].

Detection of PUFA-Modified Proteins
In Section 2, we reviewed various protein lipidations and discussed hydrophobic modification as a universal process that can regulate many fundamental biological functions. Decades ago, it was suggested that proteins can be acylated with arachidonic acid (C20:4n6) and eicosapentaenoic acid (20:5n3) [61]. Surprisingly, few studies have since reported on PUFA acylation. Only one study has described the arachidonoyl modification on the N-terminus of lens fiber major intrinsic protein (AQP0) [166]. Possible reasons for the scarcity in publication, limitations in current methodology, and potential approaches to detect PUFA-modified proteins are discussed below.

Difficulties in Detecting PUFA-Modified Proteins
Humans are estimated to express 20,000 proteins [167,168] (https://www.hupo.org/ human-proteome-project/, accessed on 2 February 2022), and PTMs of proteins play vital roles in diverse biological processes. Protein lipidation accounts for a small fraction of PTMs and of which PUFA modification represents the minority. Therefore, detection of the low levels of PUFA-modified proteins is challenging. Furthermore, double bonds in fatty acids are relatively unstable and PUFAs, especially ω-3 PUFA, are prone to peroxidation [169,170]. The peroxidation of PUFAs may happen in vivo as a part of the biological process or in vitro during sample enrichment processes in practice.
Saturated acyl chains can tightly pack with cholesterol to form ordered microdomains, such as membrane rafts, whereas unsaturated acyl chains do not pack well with cholesterol and thus form a disordered, liquid phase [171,172]. Unlike saturated fatty acids, PUFAs can target proteins to various microdomains and require diverse protein extraction procedures due to their structural complexity.

Limitations in Current Methodology
Concurrent accurate detection of abundant and scarce proteins via MS-based, highthroughput proteomic analyses is challenging [173][174][175]. Although it is feasible to use the ABE method for the enrichment of PUFA-modified proteins, this method is specific for thioester-bond analysis, and PUFA modification of proteins through other bonds cannot be detected. Detection of LDE-modified proteins was discussed in Section 2.7. It is important to distinguish proteins directly modified with LDEs from those initially modified by PUFAs and then oxidized. However, there is currently no method available for this purpose.

Potential Solutions
To characterize PUFA-modified proteins, a method that combines ABE and methyl esterification PUFA to GC/LC-MS detection and thereby detects lipidated proteins and simultaneously analyzes their fatty acid moieties is better [150,176,177]. In this method, cells are washed with PBS and then lyzed with acetone. Afterward, proteins are precipitated, and the free fatty acid content of the supernatant is characterized (GC/LC-MS A). The protein pellet is suspended, and free thiol groups are blocked with NEM. The sample is then subjected to lipid extraction with chloroform/methanol, and the free fatty acid content of the upper layer is characterized (GC/LC-MS B). The proteins in the middle layer are resuspended, divided into two, and then treated with or without NH 2 OH. The supernatant and pellet are used for the characterization of the fatty acids (GC/LC-MS C) and identification of the modified proteins (LC-MS D), respectively ( Figure 2A).
The above ABE/GC-MS method is specific to S-fatty acylated proteins. To detect other potential PUFA-protein linkages, we propose a synthesized alkynyl-linoleic acid (alk-LA) probe ( Figure 2B) in light of the synthetic method of alkynyl-palmitic acid (alk-PA) probe [178] and used the click-chemistry method for high-throughput detection of LAmodified proteins. In this strategy, the cells are incubated with the alk-LA probe for 24 h. Then, total proteins and membrane proteins are extracted, followed by the click-chemistry reaction. Afterward, the modified proteins are pulled down using streptavidin beads, digested, and finally analyzed via LC-MS ( Figure 2C) [140].
In recent years, a novel electron-transfer/higher-energy collision dissociation (EThcD) approach that preserves the original reporter ion channels and mitigates bias against the low-charge states has been proposed and optimized systematically [179,180]. This method significantly improves data quality in quantitative proteomics and proteome-wide PTM studies [181]. We think that this approach can yield a higher throughput in detecting the above-mentioned LA-modified proteins than the HCD approach ( Figure 2C). However, the general problem with "bottom up" proteomics is that some tryptic peptides are just not suitable for identification or "bad flyers", i.e., low ionization efficiency/suppression, although many solutions have been proposed, such as the EThcD, ion-mobility spectrometry (IMS) (which as a further dimension for MS analysis) [182,183], and using complementary digestion enzymes to improve sequence coverage. IMS separates ions with different conformations and charge states by guiding them through buffer gas under electric fields [184]. In recent years, researchers utilize IMS combining MS to carry out high-throughput proteomics, by this way, samples can be analyzed based on both structure and m/z to improve detection throughput. Because the IMS is much faster than LC, IMS can be inserted between LC and MS for an additional separation dimension to improve protein coverage without sacrificing the overall duty cycle/throughput [185].
Another approach is the using of "top down" proteomics to identification lipidation by MS of intact proteins. A number of intact proteins recognition technique have emerged in recent years [186], and further fueled by increase in biosimilars [187]. Generally, increased peak capacity with advanced packing material as well as longer separation columns or integrating IMS significantly improves performance in "top down" and "bottom up" proteomics [186,188].
It is noteworthy that the alk-LA probe click chemistry method also effectively distinguishes PUFA-modification from LDE-modification since only LA-acylated proteins can be pulled down in this method. To minimize the peroxidation of the LA moiety of modified proteins, the samples should be supplemented with antioxidants.   In addition, whether PUFA-acylated proteins are tethered onto cellular membranes can be determined. For this purpose, the membrane fraction of the samples should be enriched first. PUFA-modified proteins may be concentrated by taking advantage of their double-bonded feature.

Conclusions
In this review, we provided potential approaches to detect PUFA-modified proteins. Nevertheless, much remains to be explored. For instance, it is unclear how many proteins can be PUFA-lipidated and under what circumstances; what functionality PUFA modification confers to proteins; and whether ω3 and ω6 PUFA modifications differ in functionality. We hope that this review will generate interest in the research community to further study protein lipidation.
Author Contributions: Conceptualization, methodology, investigation, visualization, and writingoriginal draft preparation, R.W.; validation, formal analysis, writing-review and editing, supervision, and funding acquisition, Y.Q.C. All authors have read and agreed to the published version of the manuscript.

Conflicts of Interest:
The authors declare no conflict of interest.