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Combined effects of unloading and Cyclosporin A on myosin heavy chain in slow muscles of rats

In skeletal muscle, calcineurin has been associated both in the molecular regulation of fiber‐type expression, and in phenotypic responses to altered neuromuscular activity. A decreased calcineurin activity could be involved in the unweighting‐induced slow‐to‐fast myosin transition. This study examines both specific and combined effects of unloading and cyclosporin A (CsA) treatment, a well‐known inhibitor of calcineurin, on the distribution of myosin heavy chain (MHC) isoforms in soleus (SOL) and adductor digitorum longus (ADL) muscles of rats. Female rats were randomly assigned to 4 groups (n=8 in each group): weight‐bearing or hindlimb‐unloaded, treated either by CsA (CsA‐WB, CsA‐HU) or placebo (WB, HU) daily for 3 weeks. The MHC profile of SOL and ADL was analysed by SDS‐PAGE electrophoresis and calcineurin activity estimated by mRNA levels of modulatory calcineurin interacting protein 1 (MCIP‐1).