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[Astragalus polysaccharide combined with cisplatin inhibits growth of recurrent tumor and down-regulats the expression of CD44, CD62P and osteopontin in tumor tissues in mice bearing Lewis lung cancer].

Objective To investigate the effects of Astragalus polysaccharide (APS) combined with cisplatin (DDP) on the pathological morphology of recurrent tumor and the expression of metastasis-related proteins CD44, CD62P and osteopontin (OPN) following Lewis lung carcinoma (LLC) surgery. Methods LLC cell suspension was injected subcutaneously into palmula of left hind limb of C57BL/6J mice as a tumor-supply group. The other 80 mice were randomized into 8 groups: model group, APS-treated groups at different concentrations of 50, 100 and 200 μg/mL, 6 mg/kg DDP-treated group, and 3 mg/kg DDP combined with 50, 100, 200 μg/mL APS-treated groups. The palmula tumor cells were collected from the tumor-supply group 10 days after LLC injection and then injected subcutaneously into all of the 80 mice to establish the recurrent and metastatic mouse models of lung cancer. Subsequently, corresponding different substances were administrated intraperitoneally in the different treated groups since the next day. After 15 days' administration, all the mice were sacrificed by cervical spine dislocation. Morphological characteristics were observed by H&E staining, and the protein expression of CD44, CD62P and OPN were measured by immunohistochemistry. Results Compared with the model group, the pathological changes of the recurrent tissues in each treatment group were alleviated to some extent, especially in the DDP combined with 200 μg/mL APS group; the expression of CD44, CD62P and OPN in each treatment group decreased, especially in the DDP group and DDP combined with 100 and 200 μg/mL APS-treated groups. Conclusion APS combined with DDP could significantly inhibit the growth and metastasis of Lewis lung cancer cells, which might be associated with the reduced expression of CD44, CD62P and OPN.