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GREEN SYNTHESIS OF SILVER NANOPARTICLES USING AQUEOUS EXTRACT OF ACALYPHA INDICA AND ITS ANTIMICROBIAL ACTIVITY

In the present study, bio synthesis of silver Nanoparticles using aqueous extract of Acalypha indica and its antimicrobial activity against different micro organisms were investigated. About 10 ml of aqueous extract of A. indica added with 90 ml of AgNo3 (1mM) solution, the resulting mixture was incubated at 37 ◦ C under static condition. The development of yellowish brown color indicated the formation of Ag-Np’s. The Ag-Np’s monitored with the help of UV-visible spectrophotometer at the wavelength of 200– 800 nm. The observed absorbance peak at 400 nm indicated the formation of Ag -Np’s. The particle size of Ag-Np’s was determined by using particle analyzer and the results showed that average size range was found to be 0.516 nm.TEM technique was employed to visualize the size and shape of Ag-Np’s. The antibacterial activity of A. indica Ag-Np’s was evaluated against both Gram positive and Gram negative pathogenic microorganism by disc diffusion method. The diameter of inhibition zone of A. indica Ag-Np’s was analyzed at different concentrations ranging from 100 to 300µg/ml. The maximum zone of inhibition was observed with P.aeruginosa (16 mm), followed by E.coli (14 mm), B.subtilis((13 mm) and S.aureus (13 mm) when compared with standard drug Amikacin. The antifungal activity of A. indica Ag-Np’s at 300 µg/ml concentration was found to be 23mm and12 mm for C. albicans & A. niger respectively when compared with the standard antifungal drug ketokonazole. It is observed from the results that biologically synthesized Ag-Np’s from A. indica aqueous extract showed effective antimicrobial and antifungal activity against selected microorganisms which are comparable with standard.