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Spatiotemporal endometrial transcriptome analysis revealed the luminal epithelium as key player during initial maternal recognition of pregnancy in the mare

During the period of maternal recognition of pregnancy (MRP) in the mare, the embryo needs to signal its presence to the endometrium to prevent regression of the corpus luteum and prepare for establishment of pregnancy. This is achieved by mechanical stimuli and release of various signaling molecules by the equine embryo while migrating through the uterus. We hypothesized that embryo’s signals induce changes in the endometrial gene expression in a highly cell type-specific manner. A spatiotemporal transcriptomics approach was applied combining laser capture microdissection and low-input-RNA sequencing of luminal and glandular epithelium (LE, GE), and stroma of biopsy samples collected from days 10–13 of pregnancy and the estrous cycle. Two comparisons were performed, samples derived from pregnancies with conceptuses ≥ 8 mm in diameter (comparison 1) and conceptuses ≤ 8 mm (comparison 2) versus samples from cyclic controls. The majority of gene expression changes was identified in LE and much lower numbers of differentially expressed genes (DEGs) in GE and stroma. While 1253 DEGs were found for LE in comparison 1, only 248 were found in comparison 2. Data mining mainly focused on DEGs in LE and revealed regulation of genes related to prostaglandin transport, metabolism, and signaling, as well as transcription factor families that could be involved in MRP. In comparison to other mammalian species, differences in regulation of genes involved in epithelial barrier formation and conceptus attachment and implantation reflected the unique features of equine reproduction at the time of MRP at the molecular level.

RNA-seq data shows a strong cell type-specific endometrial response towards embryo signaling. First, a principal component analysis was performed with all samples from all cell types and days of C and P that passed the quality control (74 samples) (Fig. 1). A very clear grouping was found with a clear separation of stromal from the epithelial cells in the first dimension. In the second dimension, LE and GE were clearly separated. Analysis of DGE was performed for each cell type between P and C.
An initial analysis for each cell type between all P and C samples including all different days of P showed that P samples with small conceptuses (≤ 8 mm, mainly earlier stages of P) had expression profiles very similar to C samples, while P samples with larger conceptuses (≥ 8 mm, mainly later stages of P) showed quite distinct differences to the C samples (Fig. S3). Considering this finding, the main approach for analysis of DGE was based www.nature.com/scientificreports/ on two different comparative analyses: comparison 1; samples with larger conceptuses and clearly different gene expression profiles between P and C (LE: C n = 9, P n = 10; GE: C n = 7, P n = 10; stroma: C n = 6, P n = 12) and comparison 2; samples with smaller conceptuses and similar gene expression profiles between P and C (LE: C n = 9, P n = 7; GE: C n = 7, P n = 6; stroma: C n = 6, P n = 5).
The results of the first comparison for each cell type are shown in Fig. 2 and in the hierarchical cluster analysis in Fig. S4, revealing most differences between samples from P and C in LE with in total 1,253 differentially expressed genes (DEGs) (FDR < 1%, p-value < 0.0009), 756 upregulated and 497 downregulated in samples from pregnant mares. Less changes in gene expression were detected in GE and ST with 248 DEGs (142 up-and 106 downregulated; FDR < 5%, p-value < 0.0009) and 103 DEGs (76 up-and 27 downregulated; FDR < 13%, p-value < 0.0009), respectively ( Fig. 2 and Fig. S4B,C). The overlap of the DEGs identified in the first comparison between cell types is also shown in Fig. 2. Only 6 upregulated and two downregulated genes were found in common for all three cell types. The relative overlap between GE and LE was slightly higher (18.5%) than for ST with LE (11.7%). In addition, a few genes were found with opposite regulation in comparison of LE with ST and LE with GE. The overlap analysis is pointing at a highly cell type-specific endometrial response to embryonic signals.
The second comparison also showed mainly differential expression in LE with in total 248 DEGs (FDR 5%, p-value < 0.001), 47 with lower and 201 with higher expression in P samples compared to C samples. At the same FDR (5%), only 43 DEGs were obtained for GE (31 lower and 12 higher in P) and 64 DEGs for ST (32 lower and 32 higher in P). Differential expression analysis was also performed for LE between cyclic samples of days 10 and 13 since for this cell type 5 and 4 biological replicates, respectively, were available per day after quality filtering. This comparison revealed 377 DEGs (FDR 5%), 237 with higher expression on day 10 and 140 with higher expression on day 13 of the estrous cycle. All details for detectable genes in LE, GE, and ST are shown in Tables S4-S6, respectively.

Clustering of LE samples according to their expression profiles. A self-organizing maps (SOM)
analysis was performed to cluster all 19 LE P samples according to their expression profiles of the 1,253 DEGs obtained in comparison 1. The result of this analysis revealed grouping into 4 different clusters (Fig. 3). Figure 3 also shows conceptus sizes, twin pregnancies, and Kenney and Doig scores. Most of the samples assembled in cluster 1 or 4. Cluster #1 (n = 5) mainly contained samples where smaller embryos were found (size range of 3-7 mm in diameter) collected on P days 10 and 12. Cluster #2 included 3 samples from P days 10 and 11 and different embryo sizes (3-7 mm) including two twin pregnancies. Cluster #3 only consisted of one sample col-  www.nature.com/scientificreports/ lected on day 10 of P with an embryo of 4 mm in diameter. The samples of cluster #4 (n = 9) were mainly derived from pregnancies of later stages (except one day 11 sample, all from days 12 and 13) with embryos ranging from 8 to 26 mm in diameter. The expression profiles in cluster #1 where the most similar compared to the C sample profiles, whereas cluster #4 contained the samples with the most different expression profiles in comparison to the C group (see Fig. S5).

Functional annotation analysis and pathways of comparison 1 DEGs of LE, GE and ST.
The most significantly enriched functional terms (clusters of related terms) for genes upregulated in LE are shown in Table 1. The overrepresented functional categories belonged to biological processes involved in secretion and vesicle transport, signal transduction, cytoskeleton, cell and focal adhesion, blood vessel development, and biosynthetic and phospholipid metabolic processes. A lower number of significantly overrepresented functional annotation clusters was obtained for downregulated genes in LE. These clusters were mainly associated with lipid, steroid/sterol, and amino acid metabolic processes, morphogenesis and development, and response to growth factors (Table 2). Table S7 shows all results obtained by Metascape analysis. The genes identified as differentially expressed (DE) in GE were enriched for terms related to response to steroid and peptide hormones, extracellular matrix, carbohydrate metabolism, focal adhesion, blood vessel development, and negative regulation of immune system processes (Table S7). The functional terms overrepresented for the DEGs in ST were associated with leukocyte activation, transmembrane receptor protein tyrosine kinase signaling pathway, and lipid transport (Table S7).
A comparison of all overrepresented functional terms, biological processes, and canonical pathways obtained for the DEGs identified in LE, GE and ST by using Metascape and Ingenuity Pathway Analysis (IPA) software is shown in Fig. S6. Terms and pathways specifically enriched for LE were for example related to various signaling Results of differential gene expression analysis in comparison of samples with conceptus sizes ≥ 8 mm and cyclic controls (comparison 1). Overlap of differentially expressed genes between endometrial cell types illustrated by an Upset plot representing up-and downregulated genes obtained for luminal epithelium (LE), glandular epithelium (GE) and stroma (ST) (LE: FDR < 1%, p-value < 0.0009; GE: FDR < 5%, p-value < 0.0009; ST: FDR < 13%, p-value < 0.0009). Image created with the R package UpSetR v.1.4.0 (https:// CRAN.R-proje ct. org/ packa ge= UpSetR) 87  www.nature.com/scientificreports/ pathways, lipid metabolism, vesicle-mediated transport, and cytoskeleton organization (Fig. S6A,B). Canonical pathways specifically enriched for LE and with a positive activation Z-score (activated according to the regulation of the corresponding DEGs) were, e.g., IL-15 production, neuregulin signaling, ErbB signaling, signaling by Rho family GTPases, Wnt/β-catenin signaling, and TGF-β signaling (Fig. S6C). Furthermore, several canonical pathways with a negative activation Z-score were found, e.g., cholesterol biosynthesis, RhoGDI signaling, and PPARα/RXRα activation (Fig. S6C). Functional terms and pathways specifically overrepresented for GE and ST were related to NABA core matrisome, hexose metabolic process, apoptosis, activation of blood cells, and immune response signaling (Fig. S6).

Upstream regulator analysis for each cell type. Potential upstream regulators of the DEGs identified
in comparison 1 were obtained with Metascape 24 and IPA software (Fig. S7). The IPA upstream regulator analysis revealed several factors as significantly overrepresented and with a positive Z-score for LE, such as CREB1, TNF, interleukin 1 beta (IL1B), epidermal growth factor (EGF), TGFB1, leukotriene D4, ERK, VEGF, RAF1, beta-estradiol (E2), and MAPK3 (Fig. S7A). In LE, EGF and IL1B mRNAs were found as upregulated (log2 fold change 4.6 and 1.0). Interleukin 1 beta (IL1B) was also found as significant and with a positive Z-score for GE. In contrast, TGFB1 had a negative Z-score for GE. Specific overrepresentation and activation for GE was found for FOXO3 and KRAS. Progesterone was specifically overrepresented and activated for stroma. Metascape analysis revealed genes regulated by estrogen receptor 1 (ESR1) only as overrepresented for LE (Fig. S7B). In addition, genes that are regulated by Sp1 transcription factor (SP1) and RELA proto-oncogene, NF-kB subunit (RELA) were enriched for LE, GE, and ST. Genes regulated by peroxisome proliferator activated receptor alpha (PPARA), transcription factor AP-2 alpha (TFAP2A), and SP3 were enriched for LE and GE. The overlap of the target genes of the obtained upstream regulator networks for the DEGs found in LE was analyzed by using Venn diagrams (Figs. S8 and S9). The analysis of these overlaps suggested, e.g., the potential upstream regulators E2, oleic acid, EGF, and IL1B and connected downstream regulators as causal for DGE in LE. These regulator networks are shown in Fig  For genes detected as expressed in LE, log2 fold change is shown in the bar plot next to the gene symbol for LE, glandular epithelium, and stroma (from left to right). Image created with Ingenuity Pathway Analysis software v.68752261 (https:// digit alins ights. qiagen. com/ produ cts-overv iew/ disco very-insig hts-portf olio/ analy sis-and-visua lizat ion/ qiagen-ipa) 86  www.nature.com/scientificreports/ C10 and C13 was analyzed. Almost half of the DEGs obtained in comparison 2 (smaller conceptuses vs. cyclic controls) were also found in comparison 1 (Table S8). Comparative functional annotation and upstream regulator analysis (Fig. S10) revealed a high similarity of overrepresented functional terms and pathways (Fig. S10A-C) and potential upstream regulators (Fig. S10D,E). A few categories and pathways, and potential regulators were more significant for comparison 2, such as TF-related pathways, regulation of fatty acid (FA) oxidation, xenobiotic metabolism CAR (NR1I3) signaling, adrenomedullin signaling, estrogen receptor signaling, insuline like growth factor 1 (IGF1), and interleukin 6 (IL6) (Fig. S10A-E). Strikingly, several TF described as "early response genes" of the TF complex activator protein 1 (AP-1), early growth response (EGR), Kruppel like factor (KLF), and nuclear receptor families were already upregulated in LE in comparison 2.

Membership analysis to identify genes involved in prostaglandin signaling and metabolism, and mechanical sensation.
Using the webtool Metascape, a membership analysis was performed for the DEGs identified in LE (comparisons 1 and 2) with the keywords "prostaglandin" (PG), "arachidonic acid" (AA), and "mechanical". Table S9 shows the identified genes (in total 76), their regulation in LE, and assignment to various functional annotation terms. For PG, 33 genes were assigned, 12 for AA, and 37 for "mechanical". Some genes were found as associated to more than one keyword. Two of the genes known as directly involved in PG metabolism, 15-hydroxyprostaglandin dehydrogenase (HPGD) and prostaglandin reductase 1 (PTGR1), were already found as upregulated in comparison 2.
Searching for specific gene expression profiles in LE across cycle and pregnancy samples indicating potential association with MRP and establishment of pregnancy. Genes found as differentially expressed in LE between days 10 and 13 of C and between P and C in comparison 1 (Table S4) were analyzed by self-organizing tree algorithm (SOTA, Multi Experiment Viewer software 25 ). This analysis revealed 9 clusters of genes with similar expression profiles over days 10 and 13 of C and days 10, 11, 12, and 13 of P (Fig. S11). Four of these profiles were particularly interesting since they displayed: (i) genes downregulated from day 10 to day 13 of C but not from day 10 to day 13 of P ( Fig. 5, clusters 3 and 4); and (ii) genes upregulated from day 10 to day 13 of C but not from day 10 to day 13 of P ( Fig. 5, clusters 5 and 6). Particularly, cluster 4 contained genes found already as upregulated in comparison 2. www.nature.com/scientificreports/ The DEGs in LE with the specific profiles shown in Fig. 5 were further filtered based on expression differences between days 13 of P and day 13 of C (log2 FC ≥ 1) to obtain genes which expression was either decreasing from day 10 to day 13 of C but unchanged or upregulated in P samples ("P13 up") or increasing from day 10 to day 13 of C but unchanged or downregulated in P samples ("P13 down") (Table S10).
Upstream regulator analysis for these two groups of genes revealed basically the same factors as identified for the DEGs in LE in comparison 1 (Fig. 4). Results of Metascape analysis are shown in Fig. 6. Overrepresentation analysis for transcription factors revealed FOXA1, E2F1 and MYCN (Fig. 6A,B P13 down) as potential regulators of the genes upregulated on day 13 of C (Fig. 5, clusters 5 and 6) and SP1, ATF2-4, TST1 (POU3F1), SRF, and ESR1 (Fig. 6A,B P13 up) as potential regulators for the genes downregulated on day 13 of C (Fig. 5, clusters 3 and 4). Furthermore, the overrepresentation analysis of functional categories showed specific functional terms for the two groups of genes ( Fig. 6C and Fig. S12).
The genes assigned to "P13 up" and "P13 down" contained a strikingly large number of transcription factors (TF), e.g., members of the SRY-box TF family, KLF family, and nuclear receptor family (NR4A and NR2F) among others (Table S10). The latter family contained also nuclear receptor subfamily 2 group F member 2 (NR2F2, found as upregulated in LE comparison 1 and 2) which was found to interact with many other DEGs in LE, based on IPA interaction network analysis (Fig. S13). NR2F2 expression levels were low in GE at similar levels compared to LE of cyclic samples and high in stroma in both cyclic and pregnant samples (Tables S4-S6). Expression of many of the above-mentioned TF was already upregulated in LE in comparison 2 (Table S4 comparison 2 and Table S10).  Fig. 4 were selected based on the expression differences between days 13 of P and day 13 of C (log2 FC ≥ 1) to obtain genes which expression was either decreasing from day 10 to day 13 of C but unchanged in P samples (clusters 3 and 4, "P13 up") or increasing from day 10 to day 13 of C but unchanged in P samples (clusters 5 and 6, "P13 down"). Metascape analysis was performed for the corresponding gene lists. (A) Potential upstream regulators (TRRUST database). (B) Overrepresented transcription factor binding sites (Transcription Factor Targets database). (C) Overrepresented functional terms, biological processes, and canonical pathways. The heatmaps are colored by statistical significance (-log10 of P-value) from gray (not significant) to brown (highly significant). Image created with Metascape webtool (https:// metas cape. org) 24

Discussion
Our study provides the first extensive spatiotemporal transcriptome study in the mare covering different time points during the window of MRP. The approach used in our study to unravel unknown MRP signaling in the mare was based mainly on three points: (i) up to date, it is not completely clear when MRP is occurring in the mare; (ii) it is expected that the endometrial response to conceptus signaling depends on the developmental stage of the conceptus; and (iii) previous studies on cyclic control samples collected from day 10 to day 13 showed quite similar gene expression on these days of the estrous cycle 26 . Therefore, endometrial samples were collected on days 10, 11, 12, and 13 of P (days after ovulation) from 5 mares per P day and only on days 10 and 13 from cyclic mares. Moreover, effort was directed to collect a good number of endometrial samples from these pregnancy days that could contain embryos with different sizes. Overall, the average sizes of the recovered conceptuses gradually increased from day 10 to day 13 after ovulation, but with expected variability for the same day of P as it has been described for this period of rapid growth 27 . Whilst most of the conceptuses were in the normal range, one collected on day 12 was only 4 mm in diameter, suggesting arrested development. The data of this sample was not used for DGE analysis. On the basis of the experiences in our previous studies 11 and the results of others 12,14 , a robust approach for identification of DGE was used based on a higher number of biological replicates. Particularly, the studies of Klohonatz et al. 12,14 indicated that a low number of replicates can lead to controversial results since they did not find DEGs on day 12 of P in their first study 28 , but later they even identified DEGs on days 9 and 11 of P 12 . Unfortunately, these and other authors did not provide information about sizes of obtained conceptuses. A first analysis considered all samples collected and showed that the conceptus size could have a stronger effect than the day of P. Thus, the further approach was based on performing two different comparative analyses by grouping samples with larger conceptuses (≥ 8 mm, comparison 1) and samples with smaller conceptuses (≤ 8 mm, comparison 2) and comparing both to the same controls (C10 and C13). This approach agrees with the assumption that larger conceptuses will induce a stronger endometrial response and revealing a higher number of DEGs in comparison 1 and a much lower number in comparison 2. This contrasts with Klohonatz et al. 12 , where they found the same number of DEGs on days 9 and 11 and less on day 13. However, this result was probably due to the very low number of replicates (n = 3).
One of the main findings in the present study was that (i) the main response occurred in the endometrial LE and (ii) the response is highly specific with very low overlap between LE, GE, and ST, which confirmed results of a pilot study from our group on day 12 of pregnancy 22 . Similar results were obtained for porcine endometrium on days 12 and 14 of pregnancy 20,21 . Specific gene expression in endometrial LE and GE has been reported also in human 29 and mouse 30 . The localization of the main response to the LE is in agreement with the known signaling molecules secreted by the equine conceptus and the continues migration throughout the uterus which are mainly acting on the uterine luminal surface 2 .
The much lower number of DEGs observed in comparison 2 (small conceptuses) is also in agreement with our previous study 11 , where no DEGs could be detected on day 8 of pregnancy compared to day 8 of the estrous cycle. In our previous studies 18,19 , we also compared DGE between days 12 and 16 of P revealing a great overlap of DEGs and just more pronounced differences on day 16 compared to the day 12 cyclic controls. Since the study of day 8 of P 11 was not performed with dissected endometrial samples, it could be possible that DGE occurs in LE already on day 8 or 9, i.e., when a conceptus < 3 mm is present. Further studies on this earlier window with the LCM-RNA-sequencing approach could show if there is an earlier response than so far expected.
The following paragraphs are discussing the results of data mining with respect to the potential association of the identified DEGs with MRP and establishment of pregnancy. Because the observed gene expression changes were on the transcript level, the interpretations have some speculative character and are rather intended to provide starting points for in depth functional studies.
Overall, functional term overrepresentation analysis suggested several molecular pathways as involved in embryo sensing and establishment of pregnancy. Presumed effects of E2 and other factors known to be secreted by the conceptus and the effects of the close mechanical contact to the conceptus capsule, were reflected in corresponding overrepresented functional terms for genes up-or downregulated in LE, e.g., enrichment of signaling pathways and response to growth factors in the genes upregulated in LE of pregnant mares. Several members of a novel signaling pathway found in LE cells in sheep 31 , named JAK-SRC kinase-EGFR-RAS-RAF-ERK1/2-early growth response (EGR)-1 signaling module, were upregulated in LE of pregnant mares (e.g., EGF, MAP2K1, MAPK13, MAP3K21, EGR1-3), most of them in both comparison 1 and 2. This signaling pathway is involved in prevention of pulsatile release of PGF2a via regulation of PG transporter protein in ovine endometrium 31 . Regarding further regulatory pathways involved in MRP in other species, IRF2 mRNA was found as specifically upregulated in LE similar to ovine 32 and porcine endometrial LE on day 12 of pregnancy 33 and upregulation of ESR1 mRNA was observed on day 13 of the estrous cycle but not day 13 of P (log2 FC = -1.6) in agreement with data from day 13.5 15 . Together these findings suggest similar signaling pathways but leaving the question which signaling molecule is inducing these responses in the LE. As in other mammals, oxytocin receptor has been shown to mediate the pulsatile release of PGF2a in the mare after induction by oxytocin 34 . In contrast to pigs and ruminants, OXTR mRNA was upregulated in LE of pregnant mares, in agreement with a previous study showing downregulation of OXTR at the protein level 35 . However, OXTR expression increased between days 14 and 21 of P suggesting that OXTR expression increase is only delayed and other components are involved in preventing luteolytic pathway generation 35 . De Ruijter-Villani et al. suggested that downregulation of prostaglandin F receptor (PTGFR), which they found on days 14 and 21, as a mechanism to uncouple the oxytocin-PGF2α feedback loop 35 . Here, we found only very low expression of PTGFR mRNA regardless day or stage.
The overrepresentation of cell adhesion, cell junction, and cytoskeleton organization for genes upregulated in LE could be related to the plasma membrane transformation in LE of human endometrium that facilitates www.nature.com/scientificreports/ endometrial receptivity and implantation 36 . The plasma membrane transformation shows parallels to the epithelial-mesenchymal transition and includes morphological and molecular alterations involving the actin cytoskeleton, remodeling of adherence and tight junctions, mucins, integrin expression, and epithelial-stromal communication. In equine LE, various genes known to be involved in this process were found as differentially expressed, e.g., genes coding for components and regulators of the actin cytoskeleton, adherence, tight and anchoring junctions, beta integrins, and mucins. In contrast to humans, several genes known as involved in human embryo implantation such as WNT5A, ITGA2, and mucin genes MUC4 and MUC2 37 showed opposite regulation or were unchanged in LE, suggesting a role in regulation of delayed implantation in the mare. Interestingly, the mRNAs of claudins 4 and 10 (CLDN4, CLDN10) show a conserved pattern of increased endometrial expression during the receptive phase in cattle, pig, horse, and human 18,38 .
In addition to PG metabolism and signaling (discussed below), genes up-or downregulated in LE related to FA and lipid metabolic processes were overrepresented. Many of these genes were involved in inositol phosphate and sphingolipid metabolism and signaling. Upregulation in endometrial LE of genes participating in sphingolipid metabolism, e.g., ceramide synthase 4 (CERS4), sphingomyelin phosphodiesterase 1 (SMPD1), and sphingomyelin synthase 1 and 2 (SGMS1/2) could have regulatory roles at the maternal-embryonic interface as shown in the mouse 39 . Mizugishi et al. 40 found in mice and humans that sphingolipid metabolic pathway regulates innate immunity at the fetomaternal interface and thereby plays a critical role in fetomaternal tolerance. Genes, involved in FA metabolism and biosynthesis of unsaturated fatty acids (UFA) could be connected to the synthesis of arachidonic acid (AA), the precursor of PGs. In porcine endometrium, downregulation of mRNA expression of genes involved in generation of AA precursors was found suggesting this as a mechanism to decrease PG production 21,41 . In contrast, many of these genes were upregulated in equine endometrial LE, which could point to a role in provisioning the conceptus with lipids for metabolism as well as morphogenesis and pattern formation 42 .
Due to their known importance for MRP, a focus of the data analysis was on genes involved in PG signaling and metabolism. In addition to the above-mentioned upregulation of OXTR mRNA, PTGER2 mRNA was found with lower levels in comparison 1. Atli et al. 43 found upregulation of PTGER2 during late diestrus (days 13.5-14 post-ovulation), early luteolysis, and pregnancy days 14-22 and a downregulation of the receptor after luteolysis. Gebhardt et al. 26 analyzed different days of the cycle and found decreased levels of PTGER2 mRNA on day 16 and increased levels on days 8 and 12 post-ovulation. While diverse functions of PTGER2 in endometrium of different mammals have been suggested, a recent study showed induction of prostaglandin endoperoxide synthase 2 (PTGS2) and growth factor gene expression after PTGER2 activation in bovine endometrial epithelial cells 44 .
Phospholipase A2 (PLA2) family members have been suggested to be involved in regulation of endometrial PGF2a synthesis in the mare and DGE of PLA2 isoforms has been shown during estrous cycle and early pregnancy 45,46 . In the present study, phospholipase A2 group IIA (PLA2G2A) mRNA was decreased in comparison 1 and PLA2G4A mRNA was decreased in LE on day 13 in comparison of pregnant and cyclic mares, further supporting a role in controlling PGF2a synthesis and luteolysis.
Another way to regulate PG effects is via PG transporters to control local PG concentration. The increased mRNA expression of SLCO2A1 in comparison 1 in LE indicated a role in regulation of PG transport, particularly of PGE2 and PGF2a in LE. In the pig, this gene was also found as upregulated in LE 33 . Our previous studies showed differential expression of SLCO2A1 mRNA during the estrous cycle with lowest expression on days 3 and 8 and highest on day 16 26 . In contrast, Atli et al. 43 and Ruijter-Villani et al. 35 did not detect differences during the period of MRP and the estrous cycle, respectively. However, these authors did not perform cell type-specific expression analysis and did not analyze the same days of C. In pigs, SLCO2A1 is mainly expressed in endometrial LE and blood vessels 21,47 , and an important role in regulation of PG transport at the fetomaternal site and establishment of pregnancy has been suggested 47,48 . In humans, upregulation of SLCO2A1 expression has been found in decidual stromal cells 49 , and the authors showed that PG uptake (mainly PGE2) by decidual cells is mediated by SLCO2A1. Thus, in the mare SLCO2A1 could have a similar function in LE. Another PG transporter, ATP binding cassette subfamily C member 1 (ABCC1), was also found as upregulated in comparison 1 in LE. In the same line, ABCC1 expression was mainly localized in LE and GE and was increased by E2 in pregnant gilts 48 .
In addition to synthesis and transport of PG, degradation is another option for PG regulation. The observed regulation of two genes involved in PG degradation (PTGR1 upregulated in LE in comparison 1 and 2; HPGD only in comparison 2) suggests this option as a way to prevent PGF2a release from the endometrium in the mare. Both genes are involved in degradation of PGE2 and other PGs where PTGR1 is catabolizing 15-keto-metabolites resulting from initial oxidation catalyzed by HPGD into the fully inactive 13,14-dihydro-15-keto-metabolites 50 . Prostaglandin reductase 1 also catalyzes the oxidation of leukotriene B4 (LTB4) to inactive12-keto-LTB4 50 . Altogether, the DGE identified in the LE indicated regulation of PGE2 receptors, PG precursor generation, PG transport, and PG metabolism as potential processes involved in regulating endometrial PGF2a release into the circulation.
The results of the regulator network analysis indicated complex effects and suggested in addition to E2, cytokines (e.g., IL1B, TNF or IFNG) or substances with similar effects as well as FAs or other lipids as potential regulators of the embryo signaling response. The fact that some classical downstream regulators of E2 and other factors were not found as upregulated or even downregulated or not detectable at mRNA level, suggested that several signaling molecules are involved affecting each other's downstream regulatory network. For example, ERRFI1, strongly upregulated in LE in this and in a previous study 16 , has been described as critical for implantation in mice by suppressing ESR1 activity in the uterine epithelium and mediating P4′s suppression of E2 signaling during embryo implantation 51 . Furthermore, ERRFI1 is acting as a negative feedback regulator of EGF receptor signaling and is stimulated by epidermal growth factor 52 which mRNA was highly upregulated in LE in P.
To identify specific candidate genes involved in regulation of MRP in the mare, a focus of the data analysis was on genes which showed already DGE between P samples with smaller conceptuses (comparison 2) and on www.nature.com/scientificreports/ genes with specific expression profiles across the analyzed days of C and P. Genes with early up-or downregulation and genes with increasing or decreasing expression on day 13 of C while unchanged for P days could be involved in establishment of pregnancy and possibly in MRP.
Particularly the genes upregulated in P in comparison 2 were highly overrepresented for TF. Many of those belonged to the so-called early response TF such as the AP-1 complex subunits (FOS, FOSB, JUN, JUNB), KLF members, EGR1-3, and nuclear receptors. This could be another indication that signals of conceptuses ≤ 3 mm in diameter are inducing the first endometrial response since these factors are known to be rapidly and transiently induced after signaling by various external stimuli 53 .
Four members of the KLF TF family (KLF2, KLF4, KLF6, KLF9) showed a similar expression profile in LE with decreasing levels on day 13 of C but increased levels on day 13 of P. KLF6 and KLF9 showed already higher expression in LE of P samples in comparison 2. The expression profiles and high overrepresentation of KLF TF are suggesting an important role in the LE in establishment of pregnancy. Since KLF6 has been shown to inhibit ESR1-mediated cell growth in breast cancer cells 54 , it could be involved in suppression of estrogen effects on cell growth in equine endometrium. Zhang et al. 55 showed that KLF9 and PGR are coregulatory proteins mediating P4 responsiveness of target genes in endometrial cells. A KLF9 knockout in the mouse resulted in subfertility, uterine hypoplasia, and partial P4 resistance showing the general importance of KLF9 expression in the endometrium during pregnancy 56 . In human endometrium, PGR agonists induced KLF4 expression leading to inhibition of cell proliferation, suggesting KLF4 as a mediator of anti-proliferative effects of P4 57 . The family members KLF2 and KLF4 are belonging to the group of mechanosensitive TF 58 , pointing at a possible regulation by the mechanical stimulus of the migrating conceptus.
Furthermore, DGE of several members of the SRY-box TF family were identified in LE. The most interesting seemed to be SOX17, which has been found as upregulated in human endometrial epithelial cells by combined estrogen and P4 during the receptive window 59 . Knockdown or inhibition of SOX17 prevented trophoblast cell attachment 59 , and a decrease in the number of implantation sites was found in Sox17 heterozygous mutant mice 60 . Although, the actual conceptus implantation in the horse is happening much later, SOX17 expression in LE was increased on day 13 of P and decreased from day 10 to day 13 of C. This suggests a role of SOX17 in establishment of pregnancy but not directly in attachment of trophoblast cells to the LE.
The TF nuclear factor, interleukin 3 regulated (NFIL3) has been shown to be essential for the development of a subset of uterine natural killer (NK) cells secreting embryo growth-promoting factors in both humans and mice 61 . Furthermore, in rat granulosa cells, NFIL3 overexpression inhibited the induction of prostaglandinendoperoxide synthase 2 (Ptgs2), progesterone receptor (Pgr), epiregulin (Ereg), and amphiregulin (Areg) and down-regulated levels of prostaglandin E2 62 . The upregulation of NFIL3 in LE of P in both comparison 1 and 2 suggests a role in uterine NK cells and regulation of other key genes in equine endometrium.
A particularly pronounced transcript level upregulation in LE of P samples in both comparison 1 and 2 was obtained for the three nuclear receptor subfamily 4 group A members NR4A1, NR4A2, and NR4A3. Findings in human endometrium suggest a regulatory role of these TF also in the mare, leading to a "decidualization-like" differentiation state of the LE. Jiang et al. 63 found NR4A1 as essential for decidualization. NR4A receptors regulate forkhead box O1 (FOXO1) expression, which is mediating NR4A-induced decidualization 63 . Many decidualization-associated genes are NR4A-dependent via FOXO1, e.g., prolactin and IGFBP1. Upregulation of FOXO1 (comparison 1) and IGFBP1 (comparison 1 and 2) was found in LE of pregnant mares in the present study. Another role of NR4A1 has been described in PGR-driven endometrial vascular permeability where NR4A1 acts downstream of PGR in the regulation of the endothelial barrier function 64 . Several studies showed that members of the NR4A family are regulated by PGs. Prostaglandin F2 alpha and the PTGER2 agonist Butaprost have been shown to induce NR4A1 expression in human embryonic kidney cells (HEK293) 65 . Prostaglandin E2 has been shown to upregulate NR4A2 expression 66 . Furthermore, several studies showed that PGs are transactivating NR4A members by binding to these nuclear receptors, e.g., PGA2 binding to NR4A3 67 , and PGE1 and PGA1 binding to NR4A1 68 . The known roles of these TF in endometrium and the observed expression profiles in equine LE suggest a regulatory role in establishment of pregnancy controlled by conceptus PGs, which needs further mechanistic investigations.
In addition to the NR4A members, NR2F2 was found as upregulated in LE in comparison 1 and 2, adding it to the list of TF genes stimulated already by smaller conceptuses. The TF NR2F2 has been shown to mediate progesterone-Ihh signaling to regulate decidualization in mice 69 . In murine endometrium, stromal NR2F2 also promoted PGR expression to mediate P4-induced suppression of E2 activity in the epithelium 69 . It can be speculated that NR2F2 upregulation in equine LE is a species-specific mechanism to suppress E2 action and ESR1 upregulation.
Although, the process of decidualization is restricted to animals with invasive implantation phenotypes 70 , a number of genes described as involved in decidualization in mice and human were found as DE in LE in the mare. In agreement with the role of stathmin-1 (STMN1) in the regulation of implantation and trophoblast invasion in the mouse and in humans 71,72 , the downregulation in equine LE of pregnant mares could be a way to prevent conceptus attachment and implantation. To facilitate an intact endometrial barrier and proper support of conceptus growth by endometrial secretions, regulation of cellular junctions is important. Adrenomedullin (ADM), which mRNA was increased in LE of pregnant vs. cyclic mares (significant in comparison 1 and tendency in comparison 2) has been shown to regulate water permeability and cell junctions in the epithelial and stromal compartments of the mouse uterus during peri-implantation time 73 . The DGE of members of the claudin family observed here could also be involved in this process, because claudins 3 and 10 showed different distribution patterns during decidualization and trophoblast invasion in mouse and human endometrium 74 , and CLDN4 has been suggested to be involved in regulation of water and ion transport through the LE 75 . Additional genes with known roles in decidualization and implantation were dual specificity phosphatase 1 (DUSP1), EGR1, integrin subunit beta 8 (ITGB8), and norrin cystine knot growth factor NDP (NDP) 76 www.nature.com/scientificreports/ and non-invasive implantation in the mare, many genes known to have negative effects on decidualization and invasive implantation were found as upregulated in LE of pregnant compared to cyclic mares, e.g., parathyroid hormone-hike hormone (PTHLH) 80 , regulator of G protein signaling 2 (RGS2) 81 , and tissue inhibitor of metalloproteinases-3 (TIMP3) 82 .
In conclusion, the use of a spatiotemporal LCM-RNA-seq approach revealed the LE as the main player in the response to embryo signaling during the time of MRP. A very early response at the mRNA expression level in the LE was already found for a conceptus size of 3-4 mm in diameter. Overall, we identified a high number of DEGs and their potential upstream regulators in response to embryo signaling in LE, but also in GE and ST, pointing to key players in establishment of pregnancy. Specifically, data mining revealed regulation of PG transport and metabolism (synthesis and degradation, as well as several highly overrepresented TF families that could be involved in prevention of the release of luteolytic PGF2a and in establishing a receptive endometrium supporting conceptus growth and development. Finally, the results highlighted the uniqueness of establishment of pregnancy in equines at the gene expression level, pointing at key genes involved in epithelial barrier formation and prevention of conceptus attachment and implantation. Although our study is limited to the transcript level, since the small amount of material provided by the LCM makes it very difficult to perform proteomics analysis, it provides a broad basis for further functional studies. Ongoing experiments on the conceptus side (mRNA and proteins) and the uterine extracellular vesicles (and their molecular cargo) will add valuable information and help to unravel the molecules playing a key role in MRP in the mare.

Methods
Animal trial and sample collection. All methods were carried out in accordance with respective guidelines and regulations. The animal experiments were performed based on the permission of the Institutional Animal Care and Use Committee of the University of Illinois Urbana-Champaign (Protocol No.16129). The study was carried out in compliance with the ARRIVE guidelines (https:// arriv eguid elines. org/). Sample collection was performed with 14 light breed mares during 51 cycles of the 2018 breeding season of the northern hemisphere. The mares were housed on pasture at the Veterinary Medical Research Farm of the University of Illinois Urbana-Champaign (Illlinois, USA). The cycles (3-5 cycles) of each mare were randomly assigned to 6 groups: C days 10 and 13, and P days 10, 11, 12, 13 after ovulation. Follicular as well as CL development and ovulation were monitored (daily for the days before and after ovulation) with an Ibex® EVO® ultrasound device (E.I. Medical Imaging, Colorado, USA). When a follicle of at least 35 mm of diameter in combination with a uterine edema corresponding to estrus was detected, ovulation was induced (day -2) with an intramuscular (i.m.) injection of 1.8 mg of deslorelin acetate (SucroMateTM Equine; Thorn BioScience L.L.C.; Kentucky, USA), a GnRH analogue. Mares assigned to a P group were inseminated 24 h later (day − 1) with 50 ml of fresh semen of the same fertile stallion extended with INRA96 (imv Technologies, France) (1:1). Mares assigned to a C group were inseminated with 50 ml of INRA96 24 h after ovulation induction. Uterine lavage was performed in all mares 6 h later with Lactated Ringer Solution (RLS) (Lactated Ringer's Injection, USP; Hospira; Illinois, USA). Twenty International Units (IU) of oxytocin (VET ONE; Bimeda-MTC Animal Health Inc.; Cambridge, Canada) were administered i.m. immediately after uterine lavage as well as during the next 1-2 days (max. two times per day) to avoid intrauterine fluid accumulation. The day when ovulation was observed by transrectal ultrasonography (disappearance of the ovarian dominant follicle(s) and the identification of one (or two) CL) was designated as day 0. On sampling days (10, 11, 12, and 13), 100 ml of phosphate-buffered saline (PBS) (Corning cellgro; Virginia, USA) were transferred into the uterus using an equine uterine flushing catheter (8 mm inner and 10 mm outer diameter; JorgVet, Jorgensen Labs; Colorado, USA) in the C groups or an endotracheal tube for small ruminants (13.0 mm inner and 17.3 mm outer diameter; DEE Veterinary Products) for the P groups. The flush was then processed and stored accordingly for another study. If the conceptus was not already recovered in the small-volume uterine flush, a transcervical uterine flushing was performed with 1.5-2 l of RLS, repeating this procedure 1 to 3 times if necessary. The fluid was recovered into sterile glass bottles and passed through an embryo filter (Minitube, Germany). The recovered embryos were measured, photographed and immediately snap-frozen in liquid nitrogen. Endometrial samples were obtained by performing two trans-cervical uterine biopsies via an equine uterine biopsy forceps. The first biopsy was snap-frozen in liquid nitrogen and stored at − 80 °C until LCM and RNA-Seq. The second was fixated in 4% formalin and later embedded in paraffin for histological examination. For induction of luteolysis at the end of the experiment, 250 μg of cloprostenol, an analogue of PGF2a (Estrumate®; Merck Animal Health; Germany) was applied i.m. on the corresponding sampling day.
Laser capture microdissection of target cells, RNA isolation and, RNA-Sequencing of endometrial biopsy samples. Frozen endometrial sample were embedded in optimal cutting temperature compound (OCT Compound; Biosystems, Switzerland) before performing LCM. Cryosections of 10 µm thickness were cut at -20 °C with a Leica CM1950 clinical Cryotome and mounted on 1.0 PEN NF Membrane Slides (Zeiss, Germany). Cresyl violet staining and LCM were performed as previously described 20,21 to capture the target cells [luminal epithelium (LE), glandular epithelium (GE), and stroma including blood vessels (ST)] from the sections of each slide. In brief, a modified, rapid cresyl violet staining protocol was used to stain the sections and identify the different cell types of the endometrium. First, the slides were fixed for 2 min with 70% ethanol, and quickly washed in 50% ethanol. A 1% cresyl violet solution (Cresyl Violet acetate, C5042-10G; SIGMA; USA) was used to stain the tissue sections for 30 s, and then the slides were washed by dipping them 2-3 times in 50%, 70%, 100% ethanol, respectively. Finally, the slides were dried at room temperature. RNase-free water was used to prepare all solutions. To capture the target cells ( www.nature.com/scientificreports/ AxioCam Mrm camera, 355-nm pulsed UV laser, and PALM RoboSoftware v.4.6 (https:// www. zeiss. com/ micro scopy/ int/ produ cts/ micro scope-softw are/ palm-robos oftwa re. html) was used. Once the target cells were cut satisfactory, the tissue pieces were lifted into adhesive caps (AdhesiveCap 200 clear, Zeiss). Each selected cell type was collected in a separate adhesive cap. Representative images of sections after dissecting LE and GE and collected samples visualized on a collection cap are shown in Fig. S2. Thereafter, the cells from the LCM samples were lysed by adding 50 µl extraction buffer (Arcturus™ PicoPure™ RNA Isolation Kit, Applied Biosystems, Vilnius, Lithuania) to the adhesive caps and incubation at 42 °C for 30 min. The lysates were then frozen on dry ice and stored at − 80 °C until RNA isolation. Total RNA was isolated from LE, GE and ST from each biopsy with the Arcturus™ PicoPure™ RNA Isolation Kit following the manufactures recommendations. Agilent RNA 6000 Pico assays (2100 Bioanalyzer, Agilent Technologies, Waldbronn, Germany) were used to assess quality and quantity of total RNA extracts from LE, GE, and ST samples. Samples showing a good quality (RIN > 6) were used for RNA-Seq library preparation starting from 2.5 ng total RNA and following the instructions of the Ovation® Solo Single Cell RNA Seq System kit (NuGen Technologies, San Carlos, USA). For the first amplification step, the number of PCR cycles was between 7 and 13. Each library was barcoded individually for multiplexing (5 different multiplex pools). Sequencing of the library pools was performed on in total 10 lanes of Illumina single-read flow cells on an Illumina HiSeq 2500 instrument (Functional Genomic Center Zurich).
Data analysis. The resulting sequence reads (FastQ files) from the multiplex sequencing were analyzed with various specialized tools of a locally installed version of Galaxy 83 . Initially, the first 5 base pairs (bp) from the beginning of the reads (5′ end) were removed following the recommendations of the Ovation® Solo Single Cell RNA Seq System manual. The adapter sequence if present, as well as the low-quality ends (Quality Phred Score Cutoff = 28) from each read were trimmed. Before and after trimming, the quality of the Fastq files was checked with the FastQC-tool to confirm that the previous quality procedure was performed appropriately. The tool HISAT2 was used to perform the mapping of the sequences against the equine reference genome (EquCab3.0). Based on the 8 random bases (unique molecular identifier, UMI) contained in the barcode adapter of the libraries and the mapping coordinates in the BAM files, PCR duplicates were removed with the tool NUGEN NuDUP. A read count table was generated, and reads were filtered by a CPM cut-off before performing the statistical analysis using the Bioconductor package EdgeR (https:// bioco nduct or. org/ packa ges/ edgeR/) 84 for the identification of DEGs 84 . Genes with a false discovery rate (FDR) < 1% for LE (p-value < 0.0009), < 5% for GE (p-value < 0.0009), and < 13% for ST (p-value < 0.0009) were defined as DEGs. Known and putative human orthologous genes were assigned using an ortholog database 85 in order to improve subsequent functional annotation analysis which was performed by the use of Metascape (www. metas cape. org) 24 and IPA software (https:// digit alins ights. qiagen. com/ produ cts-overv iew/ disco very-insig hts-portf olio/ analy sis-and-visua lizat ion/ qiagen-ipa) 86 .
The RNA-seq data presented in this study are openly available at NCBI's Sequence Read Archive (SRA) under the BioProject accession PRJNA748866.