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Comparison of ultracytochemical methods for glucose-6-phosphatase activity in rat pigment epithelium.

Several media for glucose-6-phosphatase were applied to rat pigment epithelium. The Wachstein-Meisel medium showed excellent localization but the sensitivity was lower than the Hugon medium. The Hugon medium showed remark able reaction product in the endoplasmic reticulum, but occasionally the end-product was observed on some of the collagenous bundles. The media containing the higher amounts of glucose-6-phosphate tended to increase patchy stainings on the endo-