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A fluorometric rate assay of peroxidase using the homovanillic acid-o-dianisidine-hydrogen peroxide system.

The oxidation of homovanillic acid by peroxidase in the presence of hydrogen peroxide is stimulated by various compounds. Among the compounds tested, o-dianisidine was found to stimulate the oxidation of homovanillic acid strongly. A rapid and highly sensitive method for the fluorometric rate assay of peroxidase using o-dianisidine as an oxidative stimulator in the homovanillic acid-hydrogen peroxide system was established. By this method, it is possible to determine peroxidase activity in the range of 0.5-18 mU/ml.