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Distinct iris gene expression profiles of primary angle closure glaucoma and primary open angle glaucoma and their interaction with ocular biometric parameters

Abstract Background This study aimed to evaluate differences in iris gene expression profiles between primary angle closure glaucoma (PACG) and primary open angle glaucoma (POAG) and their interaction with biometric characteristics. Design Prospective study. Participants Thirty‐five subjects with PACG and thirty‐three subjects with POAG who required trabeculectomy were enrolled at the Singapore National Eye Centre, Singapore. Methods Iris specimens, obtained by iridectomy, were analysed by real‐time polymerase chain reaction for expression of type I collagen, vascular endothelial growth factor (VEGF)‐A, ‐B and ‐C, as well as VEGF receptors (VEGFRs) 1 and 2. Anterior segment optical coherence tomography (ASOCT) imaging for biometric parameters, including anterior chamber depth (ACD), anterior chamber volume (ACV) and lens vault (LV), was also performed pre‐operatively. Main Outcome Measures Relative mRNA levels between PACG and POAG irises, biometric measurements, discriminant analyses using genes and biometric parameters. Results COL1A1, VEGFB, VEGFC and VEGFR2 mRNA expression was higher in PACG compared to POAG irises. LV, ACD and ACV were significantly different between the two subgroups. Discriminant analyses based on gene expression, biometric parameters or a combination of both gene expression and biometrics (LV and ACV), correctly classified 94.1%, 85.3% and 94.1% of the original PACG and POAG cases, respectively. The discriminant function combining genes and biometrics demonstrated the highest accuracy in cross‐validated classification of the two glaucoma subtypes. Conclusions Distinct iris gene expression supports the pathophysiological differences that exist between PACG and POAG. Biometric parameters can combine with iris gene expression to more accurately define PACG from POAG.

Main Outcome Measures: Relative mRNA levels between PACG and POAG irises, biometric measurements, discriminant analyses using genes and biometric parameters.
Results: COL1A1, VEGFB, VEGFC and VEGFR2 mRNA expression was higher in PACG compared to POAG irises. LV, ACD and ACV were significantly different between the two subgroups. Discriminant analyses based on gene expression, biometric parameters or a combination of both gene expression and biometrics (LV and ACV), correctly classified 94.1%, 85.3% and 94.1% of the original PACG and POAG cases, respectively. The discriminant function combining genes and biometrics demonstrated the highest accuracy in cross-validated classification of the two glaucoma subtypes.

INTRODUCTION
The glaucomas are a group of heterogeneous optic neuropathy characterized by progressive degeneration of the retinal ganglion cells. Age and elevated intraocular pressure (IOP) are important risk factors. Recent progress in genome-wide association studies, 1,2 gene expression screening 3 and proteomic analyses 4 have revealed novel genetic risk factors and biomarkers, promising to improve diagnosis and treatment strategies for glaucoma. Primary glaucoma may be classified into two main categories: primary open-angle glaucoma (POAG) and primary angle-closure glaucoma (PACG). PACG is three times more likely to cause bilateral visual impairment than POAG. 5 Hence, accurate diagnosis is paramount. PACG is associated with several ocular biometric risk factors including well-established factors such as shallow anterior chamber depth (ACD), thick lens and shorter axial length (AL), as well as the recently identified imaging-based factors such as small anterior chamber width, area and volume (ACW, ACA, ACV), thicker iris with greater iris area and curvature, plateau iris configuration and larger lens vault (LV). [6][7][8][9] In addition to the abovementioned anatomical factors, dynamic mechanisms such as altered responses of the iris to pupil dilation and the development of choroidal effusion have been implicated in the pathogenesis of PACG. These mounting observations suggest that altered iris structure and biomechanics may emerge as distinguishing features of PACG.
However, there is limited data pertaining to altered gene expression in glaucoma iris. A study involving iris specimens from Chinese patients suggests relatively higher collagen content in acute angle closure eyes compared to POAG eyes when assessed by sirius red staining. 10 In another study, SPARC (secreted protein, acidic and rich in cysteine) and collagen I transcripts were found to be elevated in PACG compared to POAG iris. 11 SPARC is a matricellular glycoprotein involved in the regulation of collagen deposition. 12 Together, these data strongly suggest elevated collagen level in the iris could be a distinct biological signature for PACG relative to POAG.
Besides collagen, vascular endothelial growth factor (VEGF) is also implicated in glaucoma. VEGF is one of the most potent promoters of angiogenesis. 13 The association between increased VEGF in the aqueous and glaucoma has been reported previously. 14,15 Moreover, a recent study demonstrated correlation between IOP and VEGF levels in the aqueous humour of acute primary angle-closure eyes. 16 In this study, iris tissue from POAG and PACG patients was examined for the expression of genes implicated in glaucoma including COL1A1, VEGF members A to C, as well as VEGFR members 1 and 2. Their potential as unique discriminating factors and their interaction with biometric parameters for distinguishing PACG from POAG were also evaluated.

METHODS Patients
Subjects were recruited from outpatient clinics of the Singapore National Eye Centre (SNEC) between March 2011 and Dec 2012. This study was reviewed and approved by the institutional review and ethics board at the Singapore Eye Research Institute (SERI) and adhered to the tenets of the Declaration of Helsinki. Written informed consent was obtained from all subjects.
Subjects requiring trabeculectomy because of poorly controlled IOP despite maximal medical therapy and/or progressive visual field loss and optic disc cupping were recruited. POAG and PACG were diagnosed in the presence of glaucomatous optic nerve head damage, defined as a vertical cup:disc (CD) ratio of >0.7, CD asymmetry of >0.2 and/or focal notching with corresponding visual field loss on static perimetry (SITA Standard algorithm with a 24-2 test pattern, Humphrey Visual Field Analyzer II: Carl Zeiss Meditec, Dublin, CA). PACG eyes had gonioscopic findings of angle closure, defined as having the posterior trabecular meshwork not visible for at least 180°on non-indentation gonioscopy, with or without peripheral anterior synechiae. Combined trabeculectomy with mitomycin C and cataract surgery was performed where clinically indicated. Subjects with evidence of secondary glaucoma were excluded. Subjects taking medication that have effects on the iris (specifically miotics, mydriatics and systemic tamsulosin) were also excluded.

Iris specimen
Iris tissue from 34 PACG patients and 30 POAG patients were collected for analysis. Iris tissues specimens were obtained from peripheral iridectomies performed as part of a standard trabeculectomy procedure. Peripheral iridectomies were performed between 10 and 2 o'clock of the superior peripheral iris. Iris specimens were collected in RNAlater solution (Thermo Fisher Scientific Inc, MA, USA) and stored at À80°C until analyses were performed.

Real-time quantitative polymerase chain reaction (qPCR)
Iris specimens were lysed by sonication and total RNA recovered with Trizol Reagent (Invitrogen Corp, CA, USA) and the RNeasy kit (Qiagen, Valencia, CA) as described previously. 17 Five nanograms of total RNA was reverse-transcribed into cDNA using random hexamer primers (Invitrogen Corp) with Superscript III reverse transcriptase (Invitrogen Corp). All qPCR reactions, comprising the Power SYBR Green PCR Master Mix (Applied BioSystems, CA, USA), were performed in triplicates in volumes of 10 μl in 384-well microtiter plates and run using the Roche LightCycler 480 System (Roche Diagnostics Corp, Indianapolis, USA). All mRNA levels were measured as C T threshold levels. ACTB was used as the housekeeping gene, determined to be the most suitable of four analysed (ACTB, RNA18S1, GAPDH, RPL13A) using the NormFinder software. 18 The average ΔC T value calculated from the POAG irises for each gene was used for calculating the fold change in gene expression in the PACG irises by the 2 ÀΔΔCT method. Primers used for qPCR are shown in Table 1.

Anterior segment-optical coherence tomography (AS-OCT)
ASOCT imaging was obtained for a total of 20 PACG and 18 POAG patients. AS-OCT was performed using the Visante OCT (Carl Zeiss Meditec AG, Dublin, CA, USA) by a single trained technician using the quad-scan mode, which captures anterior segment images simultaneously along the meridians of 45, 90, 135 and 180°. Subjects were imaged twice, first after dark-adaption (0 lux) for at least 5 min before imaging, followed by repeat imaging whilst continuously exposing the fellow eye to bright light (1700 lux, pen light).
Custom semi-automated software (Zhongshan Angle Assessment Program [ZAAP]) was used to analyse the AS-OCT images. 19 The scleral spur obtained from each image was identified and marked, and the following averages of parameters were generated from the software: iris cross-sectional area, iris volume, anterior chamber width (ACW), anterior chamber area (ACA), anterior chamber volume (ACV) and lens vault (LV). The 90°images were excluded from analysis because of lid artifacts causing poor image quality along that meridian. Average pupil diameter was measured along meridians of 45, 135 and 180°.

Statistical methods
Post-hoc power analysis was performed using the iris gene expression data. The two-sample t-test power analysis estimated that group sample sizes of 30 will achieve 96% power to detect a difference of 0.7 between the null hypothesis that two group means are 1.8 and the alternative hypothesis that the mean of group 2 is 1.1 with known group standard deviations of 0.9 and 0.7 and with a significance level (alpha) of 0.05000.
All statistical analyses were performed using the SPSS version 19 software (IBM SPSS Statistics for Windows, Version 19.0. Armonk, NY: IBM Corp. Released 2010.). To compare gene expression or biometric measurements between PACG and POAG irises, the Student's two-tailed t-test was used, and the significance of differences between the parameters was All primer sets were used under identical cycling conditions. Sequences were obtained from GenBank and accession numbers are denoted.
corrected by Bonferroni post-hoc adjustment. A P value <0.05 was deemed to be statistically significant. Gene expression (COL1A1, VEGFB, VEGFC, VEGR2) or biometric parameters (LV, ACD, ACV) that were significantly different between PACG and POAG were selected as potential predictors for a linear discriminant analysis of these two glaucoma subtypes. To differentiate between PACG and POAG and to potentially define the allocation of new observations into either group using multivariate discriminant analysis, three multivariate models were considered, based on gene expression parameters alone, biometric parameters alone or a combination of both. The discriminant model is based on a few assumptions including multivariate normality, equality of variance-covariance within each group and low multicollinearity of the variables in order to reliably predict group membership. In accordance with these assumptions, gene expression data required normalization by conversion to the natural logarithmic form. Hence, gene expression data, whether analysed alone or in combination with biometric data, were imputed in the discriminant analysis in the natural logarithmic form. To check the assumption of homogeneity of the covariance matrix within each group, Box's M test of equality was used. Multicollinearity of the parameters was examined using correlation analysis, with correlations greater than 0.80 to be excluded.
With the classification of glaucoma types as the outcome, Wilk's Lambda statistic with Chi Square Test was used to test the significance of the model. A classification functional equation was constructed which facilitates the prediction of the glaucoma type based on the value of the classification function. All cases but one cross-validation was performed to determine the accuracy and veracity of the classification function.

RESULTS
A total of 68 subjects were enrolled in the study. 35 subjects were diagnosed with PACG and 33 subjects with POAG. The mean ages of the PACG and POAG groups were 67.3 ± 7.0 and 70.4 ± 9.2 years, respectively (P = 1.10). The subjects were predominantly male (63% in PACG and 88% in POAG; P = 0.15) and mainly of Chinese ethnicity (77% in PACG and 91% in POAG; P = 2.37). Amongst the PACG subjects, the iris tissues of 34 were analysed for gene expression and ASOCT measurements were available for 20 eyes. The total number of PACG subjects analysed for both gene expression and biometric parameters was 19. For POAG, the iris tissues from 30 subjects were measured for gene expression and ASOCT was available for 18 subjects. The total number of POAG subjects with both gene expression and biometric data 15.

Differential gene expression between PACG and POAG
Iris tissues from PACG and POAG subjects were analysed for mRNA expression of COL1A1, VEGFA, VEGFB, VEGFC, VEGFR1 and VEGFR2 by quantitative real-time PCR (Fig. 1). Data from the POAG iris were used as baseline values for comparisons of fold changes in gene expression in PACG irises. Between the two glaucoma subtypes, PACG irises expressed significantly higher levels of COL1A1, VEGFB, VEGFC and VEGFR2 transcripts compared to POAG irises (Fig. 1).

Differential biometric characteristics between PACG and POAG
The ASOCT-based biometric parameters that were evaluated include ACD, ACW, ACV, LV, iris area, iris volume and pupil diameter. PACG subjects demonstrated significantly larger LV with smaller ACD and ACV compared to POAG subjects, irrespective of lighting conditions (Fig. 2). The other parameters measured were not significantly different between the two glaucoma subtypes (data not shown).

Discriminant analyses of PACG and POAG using iris gene expression and biometric parameters
Given that there are parameters in both gene expression (COL1A1, VEGFB, VEGFC and VEGFR2), and biometric measurements (LV, ACD and ACV) that were significantly different between PACG and POAG, we proceeded to use discriminant analyses to test if gene expression parameters would effectively differentiate PACG from POAG as compared to differentiation using known biometric parameters. For a fair comparison of the discriminant functions utilizing genes alone, biometrics alone or a combination of both, we analysed data from patients who have been evaluated for both set of parameters, that is, 19 PACG and 15 POAG subjects.
To test for gene expression parameters alone, the fold change values were first normalized by conversion to natural logarithm. The resulting discriminant function accounted for 62.57% of the total variability. The box plot of the discriminant function demonstrated clear separation in the distribution of the discriminant function scores for PACG and POAG (Fig. 3a). COL1A1 expression was the strongest predictor ( Table 2). The classification results revealed that 94.1% of the original cases were classified correctly into PACG and POAG groups, whilst crossvalidated classification revealed that overall, 88.2% of cases were classified correctly (Table 3).
Next, the effectiveness of biometric parameters to differentiate between PACG and POAG was evaluated.
The resulting discriminant function accounted for 43.56% of the variation, with ACD being the strongest predictor ( Table 2). 85.3% of the original cases were classified correctly into PACG and POAG groups ( Table 3). The box plot revealed decreased segregation in the discriminant function scores compared to that  based on gene expression parameters (Fig. 3b). Hence, biometric parameters alone provided less accurate grouping of PACG and POAG compared to gene expression parameters.
We next assessed the performance of the discriminant function that incorporates both gene expression and biometric data. Because ACD and ACV are related measures, discriminant functions for each of the combinations consisting of the expression of the four genes and LV with either ACD or ACV were analysed. We found that the discriminant function consisting of gene expression, LV and ACV produced the best differentiation and accounted for the highest % of variability (eigenvalue = 2.148, proportion of trace = 68.23%) compared with the function consisting of gene expression, LV and ACD (eigenvalue = 2.066, proportion of trace = 67.40%). The structure matrix for the discriminant function incorporating genes, LV and ACV as parameters, revealed that gene expression was generally stronger predictors compared to LV or ACV, with the exception of VEGFC (Table 2); 94.1% of the original cases were classified correctly into PACG and POAG, and cross-validated classification was 91.2% accurate ( Table 3). The box plot of the discriminant function demonstrated clear segregation of the discriminant function scores (Fig. 3c). Moreover, the AUC (area under the ROC curve) for the discriminant scores of the combined variables was 98.6% (95% CI: 95.4-100, P < 0.001), strongly supporting the effectiveness of this discriminant function to distinguish PACG from POAG.
Taken together, iris gene expression profile of COL1A1, VEGFB, VEGFC and VEGFR2 can discriminate  Genes only indicates the inclusion of COL1A1, VEGFB, VEGFC and VEGFR2 expression data in the discriminant analysis. Biometrics only indicates the inclusion of lens vault (LV), anterior chamber depth (ACD) and anterior chamber volume (ACV) in the discriminant analysis.
Standardized coefficient: standardized coefficient obtained from the linear discriminant analysis of each variable for the indicated functions. Proportion of trace (%): proportion of variability of the outcome explained by the indicated variables.
between PACG and POAG. However, a combination of both gene expression and biometric data, specifically LV and ACV, generated more effective crossvalidated differentiation of PACG from POAG compared to the application of either forms of measurement alone. These data therefore support the division of PACG and POAG as distinct subtypes, characterized not only by established biometric differences, but also by fundamental iris gene expression disparities.

DISCUSSION
This study is the first to report fundamental differences at the molecular level in the glaucomatous PACG and POAG irises. We described in this study that transcript expression of COL1A1, VEGFB, VEGFC and VEGFR2 was distinct between PACG and POAG irises. Moreover, our data augmented the subgrouping of angle closure based on recently described quantitative ASOCT parameters. 20 Hence, delineating the molecular disparities between glaucoma phenotypes is important for not only contributing to our understanding of disease aetiology/pathogenesis but also for clearer definition of disease subgroups.
Uncovering the underlying molecular bases that differentiates PACG from POAG will refine our understanding of the pathophysiology for the deviation of the two disease processes. However, this is complicated by genetic heterogeneity associated with all forms of glaucoma. Many chromosomic loci have been associated with PACG and POAG, but few are well characterized. A recent genome-wide association analysis identified variants in COL11A1, amongst others, as a risk factor for PACG. 21 Loci identified in relation to the risk of POAG include CDKN2B-AS1, TGFBR3-CDC7 and FNDC3B, all potentially contributing to the regulation of transforming growth factor-β (TGF-β) signalling. Since TGF-β signalling is involved in a myriad of biological processes, including fibrosis, 22 angiogenesis 23 and lymphangiogenesis, 24 these processes are therefore implicated in the pathogenesis of PACG and/or POAG. Considering that type I collagen is a major profibrotic protein whilst VEGFs, and their cognate receptors, are well established to be involved in angiogenesis and lymphangiogenesis, our findings support the involvement of these biological processes in glaucoma pathogenesis.
The importance of type I collagen in the glaucoma iris, whose expression was prominently different between the two subtypes, is implied in clinical observations of the diseases. For instance, fibrovascular scarring at the anterior chamber angle was a notable feature of IOP-induced ocular damage. 25 This scarring effect may have its origins in the organization of collagen as intimately interwoven fibres with blood vessels in the iris, [26][27][28][29][30] and its synthesis being intimately associated with the activation of these vessels. 27,29 Indeed, VEGFB has been shown to upregulate collagen genes in vascular cells. 31 Furthermore, altered iris mobility in PACG has been described, including reduced loss in iris area consistently observed in angle closure eyes in response to mydriasis compared to open angle or normal eyes 32-34 as well as the slower speed of pupil constriction in PACG eyes. 35 The underlying explanation may be that collagen fibres are major components of the relatively inextensible connective tissue scaffold in the iris. 30 Increased type I collagen in the PACG iris may render it more rigid, thus reducing its ability to stretch and compromising pupil mobility. Gene expression variations, fundamental to tissue dysfunction in diseases, may therefore be vital for Genes only indicates the inclusion of COL1A1, VEGFB, VEGFC and VEGFR2 expression data in the discriminant analysis. Biometrics only indicates the inclusion of lens vault (LV), anterior chamber depth (ACD) and anterior chamber volume (ACV) in the discriminant analysis.
understanding the presentation, pathogenesis and progression of glaucoma subtypes.
Elevated IOP has been linked to ocular ischemia as well as iris stromal necrosis in independent studies. 36,37 We postulate that these phenomena are related and damage to the iris in turn leads to the induction of a tissue repair phenotype in this tissue in glaucoma. The hypoxia accompanying ischemia is known to induce the transcription factor hypoxiainducible factor-1 (HIF-1) which triggers many processes important for tissue repair by regulating the expression of diverse genes, including VEGF and type I collagen. 38 Whilst the role of collagen in tissue repair is well established, 39 we speculate that the upregulation of VEGFB may be a compensatory mechanism to preserve the vasculature of the ischemic glaucoma iris so as to prevent further damage. 40 Hence, the higher transcript expression of collagen and VEGF genes in the PACG iris may potentially indicate greater ischemia and a higher level of hypoxia in PACG compared to POAG, which may in turn explain the poorer prognosis of PACG versus POAG. 5,41 The significance of the gene expression data suggests that a larger number of subjects for simultaneous tissue and biometric analyses is warranted to further increase the veracity of the conclusion drawn here. A caveat in this study is the assumption that the iris specimen from the superior quadrant is representative of the whole iris. It is also not clear whether the close vicinity of the site where laser iridotomies are performed in the PACG iris may have an influence on the local iris gene expression profile. Future studies involving other independent measures such as determination of VEGFB and VEGFC protein levels in glaucomatous eyes will help to verify the distinct molecular phenotypes that exist between the disease subgroups.
In conclusion, we report distinct gene expression profiles in the POAG and PACG irises which not only distinguished the two subtypes, but also enhanced the accuracy of their description when combined with known biometric characteristics. This study therefore provides evidence to support the importance of understanding the molecular differences between glaucoma phenotypes.