{'fraction_non_alphanumeric': 0.0280970625798212, 'fraction_numerical': 0.02937420178799489, 'mean_word_length': 5.077519379844961, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
HPLC Determination of Lignocaine and Its Metabolite Xylidine in Aqueous Humor

Abstract A quick, simple, and reliable HPLC method for analysis of lignocaine and its metabolite xylidine in aqueous humor was developed. Aqueous humor was precipitated by 30% 5-sulfosalicylic acid and acetonitrile, then buffered by 4 M phosphate buffer at pH 4.0, with bupivacaine as internal standard. The chromatographic method employed a reverse C18 silica AccQ-Tag column and gradient mobile phase AccQ-Tag Eluent A and acetonitrile for separation at 37°C. The sample molecules were quantified at 220 nm. The analytical recoveries ranged from 96–109% and inter-assay imprecision less than 3.5%. In the analysis of 200 aqueous humor samples, no xylidine was detected after lignocaine gel application.