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Fiber Optic Microsensor For Continuous In-Vivo Measurement Of Blood Gases

A disposable sterile fiber optic probe has been developed to continuously monitor blood gases while residing within a 20 gauge radial artery catheter. Blood pH and dissolved 02 and CO2 are measured independently in three high n.a. 115/100 micron fibers by means of fluorescent indicators embedded in specialized membranes at the probe tip. Excitation of the fluorescent dyes is performed with a pulsed Xenon bulb; fluorescence detection with silicon photodiodes; and separation of excitation and emission is accomplished with a small cube beamsplitter located between two quarter-pitch SELFOC lenses. One of these SELFOC lenses is part of the disposable connector leading to the probe, the other leads to the excitation source via 250/200 micron fibers. The disposable portion is designed to be disconnected and reconnected without loss of calibration, requiring connector throughput reproducibility of 0.25%. System drift is typically less than 2% per 24 hours, after a 30 minute warm-up. Numerous practical problems with the fibers, connectors, SELFOC lenses, background fluorescence, system calibration, accuracy and drift have been encountered and will be discussed.