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Separation efficiency of slurry-packed liquid chromatography microcolumns with very small inner diameters.

Fused silica capillaries of different diameters were slurry-packed with an identical sorption material and evaluated chromatographically. The extent of band broadening was evaluated fluorometrically at different column lengths, together with porosity measurements obtained from a fluorescence-quenching marker. Kinetic analysis of the plate-height vs velocity data reveals that the inner diameter is a very important variable in the preparation of such packed columns. Extremely high efficiencies were obtained with columns of the smallest diameter (44 ..mu..m).