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Purification of Enzymatically Produced Trehalose by Saccharomyces cerevisiae Strains Carrying Compound UV- and NTG-Induced Mutations

Here, we describe a new method for the separation of trehalose after its enzymatic production. Compound mutagenesis of the Angel strain of Saccharomyces cerevisiae using ultraviolet irradiation and N-ethyl-N’-nitro-N-nitrosoguanidine resulted in three strains with high levels of maltose consumption, as determined by measurement of the residual maltose content of the culture medium. After 48 h of cultivation, the amount of residual maltose in the non-fermentation medium was 12.63%, a seven-fold reduction compared with that obtained with the original, non-mutated strain. Mutant strain G-4C3 produced trehalose with a purity of up to 96.69%.