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Synergistic effect of TC and PE mixtures on regulation of lipolysis in 3T3‐L1 cell differentiation

Abnormal fat accumulation on the body causes chronic disease as obesity that is serious problem in present day society. We investigated the effect of TC and PE mixtures on 3T3‐L1 cell differentiation and expected that synergistic effect of both substance. We experimented 3T3‐L1 cell differentiation for 9 to 15 days. The last day of differentiation experiment, we measured adipogenesis factor, lipolysis factor and TG level through RT‐PCR, western blot and ELISA. The intracellular lipid droplets were dyed through the Oil red O staining. Glycerol release was measured during the experiment of 3T3‐L1 cell differentiation. These result suggest that TC and PE mixtures suppressed lipid accumulatioin and expression of adipogenic transcription factors. Also, TC and PE mixtures regulated lipolitic factors and increased the amount of glycerol release. In conclusion, TC and PE mixtures have regulatory ability of lipolysis on 3T3‐L1 cell differentiation and further studies in mouse models are required.