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Unleashed DNA production for transfection, vaccines, or labeling

To understand the limits of DNA synthesis and to improve the amount of plasmid DNA produced per growth process, we deregulated the replication of a plasmid that is not subject to antibiotic selection. To understand further what occurs within a host such as E. coli when deregulated plasmid replication occurs, the proteomes of wild-type and transformed cells were contrasted. We find the copy numbers of 15,000 or higher can be obtained for a 3.7 kB plasmid. Replication fidelity or host growth rate are not compromised. The proteomics suggests that among the adaptations are increased Krebs cycle activity and also factors that enable ribosomal assembly and function.