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Improved Transformation of the Halo-Tolerant Yeast Zygosaccharomyces rouxii by Electroporation

To improve the transformation efficiency of Zygosaccharomyces rouxii by electroporation, glycerol was added to the electroporation buffer and the cells were frozen at −80 °C. These alterations drastically increased transformation efficiency, and we found that competent cells can be preserved at −80 °C without decreasing their transformation efficiency for at least 30 d.