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Electron microscopic studies of organ cultures of tooth germs infected with polyoma virus.

. Electron microscope studies were made of polyoma virus infected and uninfected organ cultures of tooth germs taken from 13- or 14-day C3H mouse embryos. Cultures were examined at 18 days and at 38 or more days of in vitro age to include both morphologically transformed and untransformed odontogenic epithelium. Epithelial and mesenchymal cells were readily identified. Virus was seen in all infected cultures and in no controls. In nuclei the virus was found in both spherical and filamentous forms, while intracytoplasmic virus was only spherical in type. No virus was seen in nuclei of cells in mitosis. No difference was detected in the pattern of virus distribution between transformed and untransformed epithelial cells.