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Influence of cations on the viability of Halobacterium mediter‐ranei cells

The effects of monovalent and divalent cations on the cell viability of an extremely halophilic bacteria H. mediterranei were examined. High magnesium concentrations (≧72 mM) were required for stabilization of H. mediterranei cells, although calcium ions were more potent than magnesium in preserving viability. On the other hand, H. mediterranei cells are also able to survive in the presence of monovalent cations, such as ammonium and potassium, at least 14 days. The implications for maintaining stability and neutrality of several cell envelope components by monovalent and divalent cations are discussed.