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[Screening of α-L-Rhamnosidases and Peptidases among Actinobacterium and Bacilli].

Purpose
To carry out screening of peptidases and α-L-rhamnosidases producers among actinobacterium and bacilli.


Methods
The biochemical methods of α-L-rhamnosidase, elastase, caseinolytic, fibrinolytic and collagenase activity determination have been used.


Results
Among 31 strains of actinobacterium and 24 strains of bacilli it was not exhibited any enzyme with elactolytic activity, while a number of actinobacterium strains displayed high collagenase activity. As to bacilli, elastolytic activity was observed only in five strains, however its level is not an interest for future investigations. Bacillus subtilis 121 and 108 exerted enough high activity (0.100 and 0.092 U/mg of protein respectively).


Conclusion
The most effective producer of collagenase and α-L-rhamnosidase is actinobacterium strain 6/5 isolated from nettle zhisosphere,while peptidase with fibrinolytic activity - B. subtilis 121 and 108. We believe these strains may be perspective for further researches.