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MicroRNA‐29a inhibits proliferation and motility of schwannoma cells by targeting CDK6

MicroRNA‐29 (miR‐29) family is involved in various types of cancer regulation. Although miR‐29 family was shown to play an inhibitory role in tumorigenesis, the effect of miR‐29a expression on schwannoma cells still remains unclear. In this study, we aimed to explore the role of miR‐29 family in schwannoma. The expressions of miR‐29a, miR‐29b, and miR‐29c were detected in the Schwann tissues and cell lines using qRT‐PCR. The effect of miR‐29a, miR‐29b, and miR‐29c on cell viability, migration, invasion, and apoptosis was tested. Then, the regulatory relationship between miR‐29a and CKD6 was detected using qRT‐PCR, Western blot, and luciferase assay. Finally, the phosphorylation levels of mainly factors in JNK and p38MAPK/ERK pathways were analyzed by Western blot. The expression of miR‐29a, miR‐29b, and miR‐29c was downregulated in Schwann tissues and cell lines. Cell viability, migration, invasion were decreased, while apoptosis was increased when miR‐29a, miR‐29b, and miR‐29c overexpression. We further found that miR‐29a negatively regulated expression of CDK6. Then, knockdown of miR‐29a promoted cell viability, migration, invasion, and inhibited apoptosis in schwannoma cells by upregulating CDK6 expression. In addition, the overexpression of miR‐29a downregulated CDK6 expression by deactivation of JNK and p38MAPK/ERK pathways. Our data suggested that miR‐29a could play an important role in inhibiting proliferation and motility of cancerous Schwann cells, and may suppress tumor growth through upregulation of CDK6.