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Elicitation of Canthin-6-one Alkaloid Accumulation in Cell Suspension Cultures of Ailanthus altissima (Mill.) Swingle

Cell suspension cultures of Ailanthus altissima (Simaroubaceae) were cultivated under illumination and in the dark. Under influence of a yeast elicitor and cell wall preparations of various Phytophthora species, respectively, alkaloid formation was stimulated. Canthin-6-one and 1- methoxycanthin-6-one accumulation could be increased up to a 125-fold and 2.5-fold, respectively within 96 h by elicitors in dark grown cultures. In response to elicitors canthin-6-one is accumulated in the cells and the growth medium.