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Modulation of Phosphoinositide Hydrolysis by NaF and Aluminum in Rat Cortical Slices

Abstract: NaF stimulated phosphoinositide hydrolysis in rat cortical slices. The production of [3H]inositol monophosphate was rapid for the first 15 min of incubation with NaF, followed by a plateau. The major product detected was [3H]inositol monophosphate, although significant amounts of [3H]inositol bisphosphate and [3H]inositol trisphosphate were also produced. The stimulation of [3H]inositol monophosphate production by NaF was concentration dependent between 2 and 20 μM NaF. Addition of 10 or 100 μM A1C13 or aluminum maltol did not alter the effect of NaF, whereas at 500 μM, these aluminum preparations resulted in significant inhibition. Increasing the concentration of K+ from 5 to 20 μM potentiated [3H]inositol monophosphate production induced by carbachol but not by NaF. Incubation with 1 μM phorbol 12–myristate 13–acetate, a phorbol ester, inhibited carbachol‐induced, but not NaF‐induced, [3H]inositol monophosphate production. These results further support the hypothesis that a guanine nucleotide binding protein that can be activated by NaF is involved in phosphoinositide hydrolysis in brain. The use of NaF provides a means to bypass receptors to study intracellular regulatory sites of phosphoinositide metabolism without disrupting cells.