{'fraction_non_alphanumeric': 0.04342581423401689, 'fraction_numerical': 0.030156815440289506, 'mean_word_length': 5.010869565217392, 'pattern_counts': {'":': 0, '<': 2, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 1, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Relationship Between Urine Hydration Indices and 24 Hour Urinary Cortisol Concentration (P24-006-19).

Objectives
The aim of this study was to investigate the relationship between urine specific gravity (USG) and color (UC), and 24-hour urinary free cortisol (UFC) concentrations in an adult population.


Methods
Adult participants aged 26-45 years (N = 51, 33 females) were recruited from the central Illinois area. Participants collected their urine over a 24-hour period. Pooled urine samples were analyzed for USG and color. UFC concentration was measured using an enzyme-linked immunosorbent assay. Whole-body adiposity (%Fat) was assessed via dual energy x-ray absorptiometry (DXA). Pearson and Spearman correlations were conducted to assess bivariate relations between variables. Age and sex were also included as covariates in partial correlation analyses.


Results
Pearson correlations revealed a positive association between UFC concentrations and hydration biomarkers in both the 24-hour pooled (r = 0.57, P < 0.01) and FMU samples (r = 0.71, P < 0.01). These associations persisted following adjustment for age, %Fat. Additionally, Spearman correlations revealed a positive relationship between UFC concentrations and 24-hour pooled urine color (rho = 0.45, P = 0.01).


Conclusions
Greater urine concentration, as indicated by elevated USG and color, was related to higher UFC concentrations. Additional experimental work is necessary to determine the extent to which changes in water intake contribute to urinary cortisol and other markers of stress.


Funding Sources
USDA National Institute of Food and Agriculture, Hatch Project 1,009,249.