{'fraction_non_alphanumeric': 0.02276064610866373, 'fraction_numerical': 0.013215859030837005, 'mean_word_length': 5.369158878504673, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Vasorelaxant effect of chloroform extract from Justicia spicigera

Hypertension remains, despite major advances in understanding its pathophysiology and therapeutic approach. One condition carries high morbidity and mortality worldwide and herbal medicine has gained ground in this area of medicine. In our group we are interested in some plants used in Mexico as alternative therapies for their possible antihypertensive properties. Previously, we tested four extracts from Justicia spicigera (hexane, chloroform, methanol and aqueous) known as Muicle in our region and the results shown that the chloroform extract has the best antihypertensive effect. The present study was designed to investigate if fractions from this extract could have a vasorelaxant effect to support our hypothesis. L‐NAME rats were used as an animal hypertensive model. The chloroform extract was fractionated in a chromatography column and collected in fractions, then were tested (0.1 – 1000 μg/mL) in phenylephrine‐precontracted endothelium‐intact aortic rings. The results suggest that the vasorelaxant effect is present in at least three fractions. The vasorelaxant effect was concentration‐dependent with EC50 = 1.1456 μg/mL ± 0.2440. The next step is to elucidate the mechanism to lower the blood pressure and if some component of the extract is the responsible for this effect.