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An international collaborative study of 69-104, a reference preparation of human pituitary FSH and LH.

A report of the preparation, ampouling and collaborative study by bioassay and by radioimmunoassay to calibrate and assess the stability of human pituitary FSH and LH reference preparation 69/104 which is available as ampoules each containing identical amounts of an extract (of human pituitary FSH and LH) derived from the National Pituitary Agency preparation LER-907. On the basis of the bioassays reported, preparation 69/104 has been assigned a potency of 10 IU FSH and 25 IU LH per ampoule and it has been recommended that 69/104 be used as a standard for bioassay comparisons with comparable pituitary extracts (Ada Endocrinologica 71: 625-637, 1972). It is unsuitable for use as a standard for inter-laboratory comparisons by radioimmunoassay except with assay systems demonstrated to be specific for one hormone only.