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Medium optimization for cell biomass yield and anthocyanin production in cell suspension culture of Melastoma malabathricum

Macronutrients influence the cell biomass yield and anthocyanin production in plant cell suspension cultures. However, different species respond differently for different nutrient concentrations. In this study, we tested the effect of different concentrations of macronutrients on the cell cultures of Melastoma malabathricum L. The results showed that the addition of NH4NO3 did not show any increment in the cell biomass but it enhanced the anthocyanin accumulation. Results indicated that CaCl2.2H2O and MgSO4.7H2O played a more important role in anthocyanin accumulation than the cell growth of M. malabathricum. However, the presence or absence of KNO3 and KH2PO4 did not show any effect on either the cell biomass or the anthocyanin production.