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Konjac glucomannan and inulin oligosaccharide attenuated the progression of colitic‐associated colon carcinogenesis and modulated immune response in mice

The aim of this study was to determine effects of konjac glucomnanan and inulin oligosaccharide on tumor development and immune function using a colitis‐associated colon carcinogenesis mouse model. Male C57BL/6J (n = 36, six‐week‐old) mice were fed AIN‐93G diet and initially divided into vehicle control and AOM‐DSS groups. Mice were given an intraperitoneal injection of azoxymethane (AOM, 10 mg/kg BW) followed by three cycles of 1–1.5% (w/v) dextran sodium sulfate (DSS) in drinking water to induce colon cancer. The vehicle group was received 0.9% saline and distilled water instead. Three days after the last DSS cycle, the AOM‐DSS treated mice were randomly assigned to the following group: control (AIN‐93G diet), KGM (2%, w/w), or inulin (2%) for 9 weeks. The severity of adenocarcinoma, leukocyte phagocytic capacity, and spleen proinflammatory cytokines were determined. Results indicated that colonic adenocarcinoma occurred in all AOM‐DSS treated mice. Supplementation of KGM and inulin effectively reduced the extent of tumor invasion. AOM‐DSS treatment reduced the leukocyte phagocytic ability and increased the spleen interleukin‐1β (IL‐1β) and tumor necrosis factor‐α (TNF‐α) levels, which was partially reversed with KGM and inulin supplementation. This study suggested that supplementation of KGM or inulin (2%, w/w) during the cancer promotion period could reduce the progression of colitis‐associated colon carcinogenesis. The mechanisms mediating effects of these fibers may involve in the improvement of leukocyte phagocytic capacity and attenuation inflammatory cytokine in the spleen.