Cell Binding, Activation, Proliferation, Depletion

Simultaneous analysis of all cells in the human circulation - in one assay.




Our expertise in flow cytometry assures high-quality assessment of cellular responses in circulating whole blood. Whatever cell type you are interested in, we bring you comprehensive results.



ID.Flow provides a unique and physiologically relevant testing environment. It allows your test substance to be studied in a dynamic environment closely resembling the human blood circulation.



One of our Sci. Advisors provides a study design that suits your unique project. You can freely choose between a scientific reporting format that suits your end goal: Standard Report IND-enabling Report Tailored Report

Flow Cytometry

Biological drugs can have an immunomodulatory effect as part of their mode of action or be immunogenic, either of which can cause safety issues or modulate the drug’s therapeutic effect. It is therefore essential to know how your drug affects the immune system from both a safety and a functional perspective. Immuneed performs phenotypical analysis of all blood cell populations and subpopulations to analyze cell activation and depletion profiles. This includes neutrophils, monocytes, granulocytes as well as B-cells, T-cells, NK cells, platelets, and red blood cells. If you are interested in looking at the proliferation or differentiation of cells in presence of your drug we also offer to complement PBMC assays.  If you are not certain of which blood cells your drug binds to, our platform (ID.Flow) also provides a unique opportunity to track the binding of different blood cell populations simultaneously.


“Our lab team has vast experience in flow cytometry analyzing Neutrophils, Monocytes, Granulocytes, B-cells T-cells, NK cells Platelets and, Red blood cells”


Cell binding by ID.Flow

Freshly acquired whole blood was incubated with anti-integrin alpha 4  (2 µg/ml) or vehicle using Immuneed’s platform based on circulating blood. Blood acquired directly after collection (zero time point) and at the 4-hour time point was stained with fluorescently labeled monoclonal antibodies to detect: erythrocytes (CD235ab+, CD45-), platelets (CD41+, CD45-), T cells (CD3+), B cells (CD19+), NK cells (CD56+), monocytes (CD14+) and granulocytes (CD66b+).

Cell activation profiles by ID.Flow

The graphs to the right show data from one of our studies on fresh blood from healthy donors, where the effect of two different antibodies was studied. One of the antibodies targets CD52 and induces activation of T-cells and monocytes. The other antibody targets EGFR, and does not induce activation of T-cells or monocytes and thus displays an activation profile similar to the vehicle buffer.