TY - JOUR T1 - Nanoscale mapping of DNA dynamics in live human cells JF - bioRxiv DO - 10.1101/405969 SP - 405969 AU - Shaban, Haitham A. AU - Barth, Roman AU - Bystricky, Kerstin Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/01/05/405969.abstract N2 - Principles of genome folding and their relationship to function depend on understanding conformational changes of the chromatin fiber over time. However, analysis of bulk chromatin motion at high resolution has not yet been reported. We developed Hi-D, a method to quantitatively map DNA dynamics for every pixel simultaneously over the entire nucleus from real-time fluorescence images. Hi-D combines reconstruction of chromatin motion using computer vision and classification of local diffusion processes by Bayesian inference. We discovered that DNA dynamics in the nuclear interior are spatially partitioned into 0.3 - 3 μm domains in a mosaic-like manner which is uncoupled from chromatin compaction. This pattern was remodeled in response to transcriptional activity accompanied by a global reduction of DNA dynamics. Hi-D opens new perspectives towards understanding motion of nuclear molecules in the context of nuclear architecture. ER -