text: Dorsal root ganglia were dissected from MrgDCreERT2;Ai32 and 
  CalcaCreERT2;Ai32 mice .1 week after dosing with tamoxifen and 
  enzymatically digested at 37˚˚C for 80 minutes in dispase type II 
  (4.7 mg/mL) plus collagenase type II (4 mg/mL) (Worthington 
  Biochemical), as described previously.63 Mechanically dissoci- 
  ated cells were plated onto laminin/poly-D-lysine (R&D Systems, 
  Minneapolis, MN) treated coverslips in complete Neurobasal Plus 
  medium (Neurobasal Plus media supplemented with 2% (vol/vol) 
  B27 Plus, 1% N2, 1% Glutamax, and 1% antibiotic–antimycotic 
  [ThermoFisher Scientific, Waltham, MA]). Mouse nerve growth 
  factor (GF) (50 ng/mL; nerve growth factor (NGF), PeproTech, 
  Cranbury, NJ) and 10 ng/mL glial-derived neurotrophic factor 
  (GDNF, PeproTech) were added to the media under some 
  conditions. Cytosine b-D-arabinofuranoside (4 mM) was added to 
  the media for 24 hours the day after plating to reduce the 
  proliferation of nonneuronal cells. Media was refreshed 3 times 
  per week thereafter. Cultures were fixed for 10 minutes at room 
  temperature with 4% paraformaldehyde and subsequently 
  processed by immunocytochemistry (described earlier). 