text: Figure 5. Neurotrophic support ameliorates MrgD1 cell loss in vitro. (A–E) Representative fields of view of MrgD-YFP and b-tubulin III expression in neuronal 
  cultures of isolated MrgDCreERT2;Ai32 DRGs, 24 hours (A) or 4 weeks (B–E) after plating, with the addition of the growth factors (GFs) NGF, GDNF, both, or neither. 
  Scale bars 5 20 mm. (F) Quantification of the percentage of neurons that express MrgD-YFP under the conditions shown in (A–E). One-way ANOVA with Tukey 
  posttests; F4,15 5 39.7, P , 0.001; n 5 4 mice. Posttests:*P , 0.05,**P , 0.01,***P , 0.001. (G–K) Representative fields of view of Calca-YFP and b-tubulin III 
  expression in neuronal cultures of isolated CalcaCreERT2;Ai32 DRGs, with the addition of NGF, GDNF, both, or neither. Scale bars 5 20 mm. (L) Quantification of the 
  percentage of neurons that express Calca-YFP. One-way ANOVA; F4,14 5 0.46, P 5 0.76; n 5 3 to 4 mice. ANOVA, analysis of variance; DRG, dorsal root 
  ganglion; GDNF, glial-derived neurotrophic factor; NGF, nerve growth factor; YFP, Yellow fluorescent protein. 