text: formaldehyde. L3 to L5 DRGs were removed and postfixed for 
  another 2 hours, cryoprotected in 30% sucrose overnight, and 
  then embedded in optimal cutting temperature media (OCT; 
  Tissue Tek, Alphen aan den Rijn, the Netherlands). Dorsal root 
  ganglia were sectioned on a Leica CM1950 cryostat at 30 mm, 
  with every section collected serially on 5 Superfrost Plus slides 
  (VWR, Lutterworth, United Kingdom) and each slide containing 1 
  in every 5 sections (4-7 sections per slide). One slide per DRG was 
  selected at random and was washed with PBS, before being 
  incubated with appropriate primary antibodies (Table 2) diluted in 
  5% normal donkey serum and 0.3% Triton X-100 in PBS for 
  3 days at 4˚C. After PBS washes, slides were incubated with 
  appropriate secondary antibodies (Table 2) in the same PBS/ 
  (normal donkey serum) NDS/Triton-X100 solution as for prima- 
  ries, overnight at room temperature. Slides were washed and 
  coverslipped with VectaShield Vibrance Hardset mounting media 
  (Vector Labs, Newark, CA), with 4’,6-diamidino-2-phenylindole 
  included in mounting media where FB-labelled cells were not 
  being examined. Sections were imaged using a Zeiss LSM900 
  Airyscan confocal microscope equipped with 405-, 488-, 561-, 