text: Figure 3. Spared nerve crush or transection results in death of nonpeptidergic neurons. (A) Schematic of experimental approach for (B and C). (B) MrgDChR2-YFP L4 
  DRGs 4 weeks after SNI, contralateral or ipsilateral to injury. Images are projections of optical sections at 3-mm intervals through the entirety of 30-mm-thick tissue 
  sections. Scale bars 5 100 mm. (C) Quantification of total number of MrgD-YFP1 cells per L4 DRG 4 weeks after SNI revealed a significant loss in ipsilateral DRG. 
  Two-way RM ANOVA with ˇS´ıd ´ak multiple comparisons tests; Side x Treatment interaction: F1,5 5 9.23, P 5 0.029; n 5 3 mice. (D) The experimental approach 
  used to generate data presented in (E–G). (E and F) MrgD-YFP expression and FB labelling in the L4 DRG, 14 days after SNI or crush surgery or contralateral to 
  injury. White boxes represent regions enlarged in (F). Scale bars 5 100 mm (E) or 20 mm (F). (G) The proportion of FB-labelled DRG neurons decreased after spared 
  nerve crush injury, and co-labelling is almost completely absent after SNI. Two-way RM ANOVA with ˇS´ıd ´ak multiple comparisons tests; side 3 injury interaction: 
  F1,4 5 7.80, P 5 0.049; n 5 3 mice. Posttests:*P , 0.05,**P , 0.01. ANOVA, analysis of variance; DRG, dorsal root ganglion; SNI, spared nerve injury; FB, 
  FastBlue; RM, repeated measures. 