text: Mice were housed in groups in humidity- and temperature-controlled 
  rooms with free access to food and water, on a 12-hour light–dark 
  cycle, and with environmental enrichment. Animal procedures were 
  performed under a UK Home Office Project Licence and in 
  accordance with the UK Home Office (Scientific Procedures) Act 
  (1986). All studies were approved by the Ethical Review Process 
  Applications Panel of the University of Glasgow or Oxford and conform 
  to the ARRIVE guidelines. Experiments were performed on adult male 
  and female mice aged 7 to 16 weeks at the start of the experiments. All 
  experimental cohorts contained a mix of male and female mice, apart 
  from the cohort of MrgprdCreERT2;Ai32 mice that underwent SNIcrush 
  surgery, which was exclusively female. Details of transgenic lines are 
  provided in Table 1. Tamoxifen was administered by i.p. injection of 
  20 mg/mL tamoxifen (Sigma-Aldrich) dissolved in wheat germ oil 
  (doses described in Table 1). There were 2 instances where animals 
  were excluded from data analysis: One (cyan fluorescent protein) 
  Thy1-CFP died of unknown causes not related to the procedure and 
  before the experimental endpoint, and one MrgDCreERT2;Ai32 
  exhibited no fluorophore expression and was therefore deemed to 
  have been incorrectly genotyped. Group sizes were based on the 
  extent of neuronal 
  loss 28d following sciatic nerve transection 
  identified by Shi et al.50 Given a 5 0.05, power 5 0.8, and an effect 
  size of 4.81, power analysis projects that a group size of 3 mice would 
  be needed. 