text: A.H. Cooper et al.·165 (2024) 2863–2876 
  2866 
  
  image 
  and 640-nm diode lasers. Full thickness, tiled, confocal 
  stacks with a 2- to 3-mm interval 
  in the Z-axis were obtained 
  through a 203 dry lens (0.8 NA) with the confocal aperture set to 1 
  Airy unit or less. All image capture was performed using Zen Blue 
  Edition software (Carl Zeiss Microscopy GmbH, Jena, Germany), 
  and analyses were performed using Zen Blue or FIJI.45 
  
  2.5. Image analysis 