text: Figure 2. Spared nerve crush and transection lead to a loss of small DRG neurons. (A) Approach to restrict analysis to damaged afferents: a subcutaneous 
  injection of the tracer FB into both hindpaws labelled tibial afferents, before unilateral SNItrans or SNIcrush surgery. (B) Representative image of FB labelling and NeuN 
  immunostaining in the L4 DRG. The image is a projection of optical sections at 3-mm intervals through the entirety of a 30-mm-thick tissue section. Scale bar 5 
  100 mm. (C and D) Quantification of the cross-sectional area of FastBlue labelled DRG neurons ipsilateral and contralateral to SNItrans (C) or SNIcrush injury (D) 
  reveals a loss of small afferents and subsequent shift in population distribution. Kolmogorov–Smirnov tests of cumulative distributions; SNItrans: D 5 0.25, P , 
  0.001; n 5 183 or 191 neurons from 3 mice; SNIcrush: D 5 0.22, P , 0.001, n 5 319 or 325 neurons from 3 mice. (E) Experimental approach for whole DRG 
  volumetric analyses after SNItrans. (F) Representative 3D rendering of TDP-43 profiles and corresponding nuclear spot profiles following Imaris-based spot 
  detection feature. Scale bar 5 100 mm. (G) Quantification of DRG nuclear spot volume ipsilateral and contralateral to SNItrans. Kolmogorov–Smirnov tests of 
  cumulative distribution: D 5 0.06, P , 0.001, n 5 30,206 (contra) or 32,544 (ipsi) nuclei from 4 (contra) or 5 (ipsi) mice. (H) Total number of nuclear spots, by size, 
  per DRG. Two-way RM ANOVA; size bin 3 injury interaction: F2,145 8.26, P 5 0.004; n 5 4 to 5 mice; ˇS´ıd ´ak multiple comparisons tests:**P , 0.01. ANOVA, 
  analysis of variance; DRG, dorsal root ganglion; FB, FastBlue; RM, repeated measures. 