text: 4.4. Study limitations 
  
  Our efforts focused on traumatic nerve injury paradigms owing to 
  previous contrasting results using these robust and reproducible 
  experimental models. We did not extend our studies to systemic 
  neuropathy models, such as chemotherapy or diabetic neurop- 
  athy. A recent postmortem analysis reported a neuronal loss in 
  the DRG from patients with painful diabetic peripheral neurop- 
  athy.19 Transcriptional 
  responses vary substantially across 
  different nerve insults,44 so it would be of interest to test whether 
  neuronal loss and the subpopulation vulnerability reported in this 
  study are common features across different types of insults. 
  
  Using multiple approaches, we assess the na¨ıve mouse L4 
  DRG to contain approximately 8000 neurons, consistent with 
  a previous estimate,67 and observed a frank loss of small- 
  diameter neurons following injury. However, the extent of loss 
  observed using our semiautomated approach was less than that 
  observed using manual techniques.67 Two major limitations in 
  this study may explain this discrepancy: First, owing to technical 
  issues, the cleared DRG dataset is unpaired ipsilateral–contra- 
  lateral which adds larger variability. Second, the analysis method 
  is prone to undercounting deep nuclei. The signal-to-noise is 
  better for superficial nuclei and smaller tissue volumes. Given the 
  reduction in DRG volume after SNItrans, nuclei 
  in larger 
  contralateral DRG may be undercounted. 