Figure 3.Spared nerve crush or transection results in death of nonpeptidergic neurons. (A) Schematic of experimental approach for (B and C). (B) MrgD ChR2-YFP L4
DRGs 4 weeks after SNI, contralateral or ipsilateral to injury. Images are projections of optical sections at 3- mm intervals through the entirety of 30- mm-thick tissue
sections. Scale bars 5100 mm. (C) Quantification of total number of MrgD-YFP 1cells per L4 DRG 4 weeks after SNI revealed a significant loss in ipsilateral DRG.
Two-way RM ANOVA with ˇS´ıd ´ak multiple comparisons tests; Side x Treatment interaction: F 1,5 5 9.23, P 5 0.029; n 5 3 mice. (D) The experimental approach
used to generate data presented in (E–G). (E and F) MrgD-YFP expression and FB labelling in the L4 DRG, 14 days after SNI or crush surgery or contralatera lt o
injury. White boxes represent regions enlarged in (F). Scale bars 5100 mm (E) or 20 mm (F). (G) The proportion of FB-labelled DRG neurons decreased after spared
nerve crush injury, and co-labelling is almost completely absent after SNI. Two-way RM ANOVA with ˇS´ıd ´ak multiple comparisons tests; side 3 injury interaction:
F1,4 5 7.80, P 5 0.049; n 5 3 mice. Posttests: * P , 0.05, ** P , 0.01. ANOVA, analysis of variance; DRG, dorsal root ganglion; SNI, spared nerve injury; FB,
FastBlue; RM, repeated measures.
2870 A.H. Cooper et al. ·165 (2024) 2863–2876 PAIN®