neurons
39 The Jackson Laboratory (RRID:
IMSR_JAX:031286)
General: 1x 50 mg/kg in adulthood, (.1 week
before experiment)
3D volumetric analysis: 5x i.p. (0.5 mg/animal/
day), beginning between P10 and P17
MrgDChR2-
YFP
Mrgprdtm4.1(COP4)Mjz Major class of nonpeptidergic
neurons
59 Mutant Mouse Resource & Research
Centers (RRID:MMRRC_036112-UNC)
N.A.
CalcaCreERT2 Calcatm1.1(cre/ERT2)Ptch Peptidergic neurons 51 Gift: Prof Pao-Tien Chuang 1x 75 mg/kg in adulthood ( .1 week before
experiment)
Trpm8FlpO Cold afferents 4 Gift: Dr Mark Hoon N.A.
Thy1-CFP B6.Cg-Tg(Thy1-CFP)
23Jrs/J
Sample of myelinated afferents 16 The Jackson Laboratory (RRID:
IMSR_JAX:003710)
N.A.
ThCreERT2 Thtm1.1(cre/ERT2)Ddg/J C low threshold
mechanoreceptors
1 Gift: Prof David Ginty; The Jackson
Laboratory (RRID:IMSR_JAX:025614)
1x 50 mg/kg in adulthood (.2 weeks before
experiment)
RC::FLTG B6.Cg- Gt(ROSA)
26Sortm1.3(CAG-tdTomato,-
EGFP)Pjen/J
Flp-mediated tdTomato;
Cre1Flp-mediated GFP
expression
40 The Jackson Laboratory (RRID:
IMSR_JAX:026932)
N.A.
Ai14 B6.Cg- Gt(ROSA)
26Sortm14(CAG-tdTomato)Hze/
J
Cre-mediated tdTomato
expression
33 The Jackson Laboratory (RRID:
IMSR_JAX:007914)
N.A.
Ai32 B6.Cg- Gt(ROSA)
26Sortm32(CAG-
COP4*H134R/EYFP)Hze
Cre-mediated ChR2-eYFP
expression
32 The Jackson Laboratory (RRID:
IMSR_JAX:024109)
N.A.
CFP, cyan fluorescent protein; GFP, Green fluorescent protein; YFP, yellow fluorescent protein.
2864 A.H. Cooper et al. ·165 (2024) 2863–2876 PAIN®