 The assignment of Calostoma to the Boletales placed it in an order whose biochemistry has been the topic of research . Most members of the Boletales are characterized by compounds produced by the <unk> @-@ <unk> pathway , including several distinctive pigments . <unk> Gruber and Wolfgang <unk> were not able to detect these compounds in C. cinnabarinum , but isolated a novel <unk> pigment . This compound , named calostomal , is responsible for the orange @-@ red color of the fruit bodies . The methyl ester of calostomal was subjected to NMR spectroscopy and was identified as all @-@ trans @-@ 16 @-@ oxohexadeca @-@ 2 @,@ 4 @,@ 6 @,@ 8 @,@ 10 @,@ 12 @,@ 14 @-@ <unk> acid . Chemically related pigments , the <unk> , had been isolated from the brightly colored Boletus <unk> and B. <unk> . It is not yet clear if the results of this <unk> investigation will mandate changes to Boletales cladistics . 
