 The assignment of Calostoma to the Boletales placed it in an order whose biochemistry has been the topic of research . Most members of the Boletales are characterized by compounds produced by the shikimate @-@ chorismate pathway , including several distinctive pigments . Gertraud Gruber and Wolfgang Steglich were not able to detect these compounds in C. cinnabarinum , but isolated a novel polyene pigment . This compound , named calostomal , is responsible for the orange @-@ red color of the fruit bodies . The methyl ester of calostomal was subjected to NMR spectroscopy and was identified as all @-@ trans @-@ 16 @-@ oxohexadeca @-@ 2 @,@ 4 @,@ 6 @,@ 8 @,@ 10 @,@ 12 @,@ 14 @-@ heptaenoic acid . Chemically related pigments , the boletocrocins , had been isolated from the brightly colored Boletus laetissimus and B. rufoaureus . It is not yet clear if the results of this chemotaxonomic investigation will mandate changes to Boletales cladistics . 
