Hamburg and Berlin are well connected by an Autobahn, Google Maps indicates 3:12 hours, which seems about right, but many parts do not have speed limits, you may possibly be a bit faster than that. Train connections are with a fancy ICE or the elder train connecting Hamburg with Prague and Budapest - 1:44 hours is what Google lists, but go for the one that continue to Prague and have some good Bohemian food in the train's restaurant. You'd cycle for 16 hours or walk two days.
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