In contrast to the recent accomplishments of genome projects, cytological information on chromosomes and genomes of the genus Colletotrichum is very scarce. In this study, we performed mitotic cytological karyotyping for the three species, C. orbiculare, C. graminicola, and C. higginsianum by fluorescence microscopy and compared the results with those from genome projects. Chromosome number (CN) of C. orbiculare was determined for the first time to be n=. 10 with no minichromosomes (MCs) in the genome, while CNs of C. graminicola and C. higginsianum were consistent with those from their genome project including the number of MCs. Regarding chromosome features, C. orbiculare was peculiar in that each chromosome was distinctly partitioned into a highly AT-rich pericentromeric region and the remaining highly GC-rich regions, and the pericentromeric region was judged to be constitutive heterochromatin. Integrating all the discernible morphological characteristics such as chromosome length, nucleolar organizing region, and DAPI-stained regions, idiograms were constructed for the three species. The overall cytological features of the chromosomes and genomes fit well with the data from the genome projects in terms of genome size, GC-content, and the occurrence of AT-rich regions. This study represents the most comprehensive and detailed mitotic cytological karyotyping of fungi ever reported.
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Tofauti na mafanikio ya hivi karibuni ya miradi ya genome, habari ya cytological juu ya chromosomes na genomes ya jenasi Colletotrichum ni nadra sana. Katika utafiti huu, tulifanya mitotic cytological karyotyping kwa spishi tatu, C. orbiculare, C. graminicola, na C. higginsianum kwa microscopy fluorescence na kulinganisha matokeo na wale kutoka miradi ya genome. Nambari ya kromosomu (CN) ya C. orbiculare iliamuliwa kwa mara ya kwanza kuwa n=. Kwa mfano, kuna aina kadhaa za chromosomes katika genome ya C. graminula na C. higginus, na idadi ya chromosomes ni sawa na idadi ya chromosomes katika genome ya C. higginus. Kwa upande wa chromosome, C. orbicularis ilikuwa ya kipekee kwa kuwa kila chromosome ilikuwa imegawanywa kwa njia ya wazi katika eneo la pericentromeric lenye utajiri wa AT na maeneo mengine yenye utajiri wa GC, na eneo la pericentromeric lilihukumiwa kuwa heterochromatin ya msingi. Kwa kuunganisha sifa zote za kimaumbile kama vile urefu wa kromosomu, eneo la kuandaa nucleolar, na mikoa ya rangi ya DAPI, idiograms zilijengwa kwa spishi tatu. Uwezo wa jumla wa cytological wa chromosomes na genomes huambatana vizuri na data kutoka kwa miradi ya genome katika suala la ukubwa wa genome, maudhui ya GC, na kutokea kwa mikoa yenye AT. Utafiti huu inawakilisha zaidi ya kina na kina mitotic cytological karyotyping ya kuvu milele taarifa. ASC Scopus