CAT Hematoxylin sections in pretreated formalin-fixed, paraffin-embedded tissue cellular preparations in is used to visualize cellular nuclei in tissue sections and immunohistochemistry.
CAT Hematoxylin is a Mayer’s Hematoxylin (Lillie modification) histological staining reagent for use in the histologic demonstration of nuclear staining. It is also used in special-staining procedures as a counterstain for nuclear demonstration. Hematoxylin solutions contain hematein and a metal mordant. The mordant provides the stain color. In the case of Mayer’s Hematoxylin (Lillie’s modification), the hematoxylin contains aluminum, giving a purple color (Nuclei after Bluing: Blue). This staining technique is used to make the distinction between a normal nucleus and an abnormal one. For histochemical purposes, hematoxylin is commonly used as a progressive stain. Slides are left in the hematoxylin solution only long enough to stain the nuclei. After staining, tissues are blued in water that has been slightly alkalinized.
Hematoksilini (H) katika vifaa vya seli za tishu zilizowekwa formalin, na parafini hutumiwa kugundua nyuklia za seli katika sehemu za tishu na immunohistochemistry. Cat Hematoxylin ni Mayer's Hematoxylin (Lilli modify) histological staining reagent kwa ajili ya matumizi katika histological maonyesho ya nyuklia staining. Pia hutumiwa katika taratibu za pekee za kugeuza rangi kama counterstain kwa maonyesho ya nyuklia. Suluhisho la hematoxylin lina hematein na mordant ya chuma. Mdudu huyo hutoa rangi ya madoa. Katika kesi ya Mayer's Hematoxylin (mabadiliko ya Lillie), hematoxylin ina alumini, ikitoa rangi ya zambarau (Nuclei baada ya Bluing: Blue). Mbinu hii ya kupaka rangi hutumiwa kutofautisha kati ya kiini cha kawaida na kisicho cha kawaida. Kwa madhumuni ya histochemical, hematoxylin ni kawaida kutumika kama stain maendeleo. Slides ni kushoto katika ufumbuzi hematoxylin tu muda wa kutosha stain nuclei. Baada ya kupakwa rangi, tishu hizo hutiwa rangi ya bluu katika maji ambayo yamekuwa na alkali kidogo.