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Morocco Golf Tour
Book your luxury Morocco golf tour today! Unique challenges and remarkable vistas await you on the world-class golf courses in Morocco. As you cross fairways surrounded by magnificent palm oases, snow-dusted peaks, hundred-year-old oaks, and ancient ruins, you'll marvel at the wonder of Morocco's magnificent landscapes. Designed by masters including Robert Trent Jones, Jack Nicklaus, and Cabell B. Robinson, these courses make for memorable competition in your luxury Morocco golf tours. Carefully designed to showcase highlights of Morocco along the way, Morocco golf tour brings you a unique sporting and cultural experience.
Your luxury Morocco golf tour begins with welcome by your private driver upon your arrival at Marrakech, an ancient city of weathered pink buildings and orange groves. At Morocco golf tour, spend the afternoon with a private guide as you explore fascinating local souks (markets), ancient palaces, and lush gardens. Celebrate the start of journey by dining at a centuries-old former palace. Your world-class golfing in Morocco begins the next day at the Marrakech Royal Golf Club. Built in the 1920s, this is the oldest and arguably the most scenic course in Morocco. Cypress, eucalyptus, palm, olive, orange, and apricot trees line the fairway while the snow-capped Atlas mountains provide a majestic backdrop for your game. In the afternoon, visit the Djemaa el-Fna, a carnivalesque public square where water sellers, snake charmers, magicians, and storytellers compete for an audience. Next in the Morocco golf tour is to tackle the masterpiece 18- hole Palmeraie Golf Club. Set on a spacious valley with numerous sand hazards, abundant palm trees, and magnificent lakes, this golf course is truly a masterpiece making your golfing in Morocco memorable forever. In the evening, feast amid a spectacle of belly dancers.
Depart the next day and traverse the Middle Atlas contrasting the alpine resort town of Ifrane with ancient Berber villages. Arrive in Fez, Morocco's third largest city and the country's cultural, intellectual, and spiritual heart. By morning, luxury Morocco golf tour will let you conquer the sloping greens and undulating fairways of the Royal Golf Club of Fez, set amid olive groves and with mountain views. By afternoon, enjoy a privately guided tour of Fez and its medieval medina, a UNESCO world heritage site crammed with narrow, winding streets, tiny squares, and artisan workshops. Dine in the evening at a celebrated former palace. Next travel of your luxury Morocco golf tour will be by private car to Rabat, Morocco's present-day capital and principal royal residence. You'll spend the next day of Morocco golf tour by playing at one of the world's most famous Golf courses, the Dar-Es Salam Royal Golf Club, home to the annual Hassan II Trophy Tournament and ranked among the most spectacular in the world. Magnificently designed by Robert Trent Jones, the three courses at this club offer something for every golfer. Complete your visit to Rabat with more golf at other majestic area courses or private touring of the city's oceanfront medina, historic monuments, tranquil gardens, or fabulous beaches. Conclude your luxury Morocco golf tour in Casablanca, first visiting one of the world's most splendid monuments, the Hassan II Mosque, a stunning state-of-the-art gem of religious architecture, followed by an afternoon at the charming Royal Golf Club of Mohammedia, bedecked with delightful flowers and the first golf course built in Morocco. We'll cap off the day and luxury Morocco golf tour by dining at one of the best seafood restaurants in Casablanca.
Luxury Morocco Golf Tour Itinerary Overview
Day 1
Day 2
Day 3
Day 4
Marrakesh – Fez
Day 5
Fez –
Day 6
Fez – Rabat
Day 7
Day 8
|Day 9
| Rabat – Casablanca
Royal Golf Club of Mohammedia.
Day 10
Depart Morocco
Accommodations on this luxury Morocco golf tour
- Casablanca: Idou Anfa
- Rabat: Sofitel Diwan
- Fez: Riad Mabrouka or Riad Yacout
- Marrakesh: Riad El Assafir or Riad Bahja
- The hotels and riads listed are an example; you will stay there or somewhere of similar quality.
$3190 per person based on 2 people on your party.
- – We can craft the trip based on your budget and pace. If you can imagine it, we can make it happen!
- – Single supplements available upon request.
- – Add $300 supplement for December departures.
The total price of Morocco golf tour includes
- – Lodging at charming riads or hotels 4*
- – All transportation, including recent-model sedan or minivan with air conditioning, private English-speaking driver, and fuel
- – Government-certified tour guides in major cities
- – Entrance fee to monuments and sightseeing
- – Breakfast daily
- – Two dinners and two lunches.
- – All applicable taxes
- – Sahara Soul Travel credit of 3% upon your return (can be used by any member of your family or friend)
Not included in price
- – Air fare
- – Travel insurance
- – Lunches and some dinners
- – Drinks
- – Personal needs expenditures
- – Gratuities
Features of luxury Morocco golf tour
- – Private guided travel with English-speaking driver (not a group tour)
- – Morocco golf tour can be customized based on your interest and budget. Also, you can lengthen or shorten the trip.
- – Departs daily year-round on private basis
- – Morocco golf tours are designed for First Class (4* accommodation and services). Upgrade to Deluxe and Luxury (Elite) is available; also the trip can be altered to lower budget.
- – Group discounts available
- – 10-day trip begins in Casablanca and ends in Marrakesh
- – 9 nights first-class and best-available hotels or riads
- – Activities: golf, cultural connections, UNESCO World Heritage sites, exploration, stunning Berber villages, souks and shopping, handicraft production, formal gardens, holy sites, Roman ruins, historical sites, sightseeing, walking, authentic local culture
- – Activity level: Easy to Challenging, depending on your preference.
- – A deposit of $500 per person is required to confirm reservations for Morocco golf tours. Final payment is due 60 days prior to departure.
- – Prices quoted for land arrangements are subject to change without notice.
- – A recent-model sedan (1-2 people)
- – Luxury minivan or van (3+ people)
Book this Trip with Morocco private guides to request the complete itinerary for the above tour, or click here for Morocco customized trips to begin customizing your own itinerary.


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Football is a game for gossips. Entire columns are gleefully devoted to the rumours and scandals that seem to so regularly envelop the sport. Mourinho, claws out, called the Man City boss Mr. Pellegrino? Fantastic! Magath has been seen lurking suspiciously close to the cheese counter again? Superb! It isn't so unbelievable to think that a joyous national holiday might be declared if some poor soul forgets Yaya's birthday again.
Yet the latest offering to the veritable smorgasbord of scandals that have hit FIFA recently hasn't piqued the taste buds of those hungry for drama. In fact, its left many depressed and without appetite, wondering quite where it all went so drastically wrong.
At best, FIFA Executive Committee member Theo Zwanziger's admission that he doubted that Qatar would host the 2022 World Cup embodies the critically poor decision making that football's international governing body seems to be making a habit of exhibiting. A very, very small child could have predicted that hosting a football tournament in a country which has an average temperature of 37 degrees between May and September may cause some difficulties. At worst, however, this news highlights severe misjudgments at the very top of FIFA which beggars belief.
The very essence of sport lies in fair play. Back in 2006, when football's International Federation found itself mired in allegations of bribery corruption the expectation was that FIFA would react quickly to quell fears. Six years later, after two BBC Panorama documentaries, the threat of an International Olympic Committee (IOC) investigation and a sting from The Sunday Times, FIFA set up the bicameral FIFA ethics committee to replace the woefully inadequate single chamber version. The second generation body was conceived to investigate the allegations of bribery and vote rigging on a colossal scale during the disorderly simultaneous bidding process for the 2018 and 2022 World Cups.
In March, FIFA Chief Investigator Michael Garcia (formerly of the FBI) was the target of an attempted coup half way through the investigation which intended to oust him and disband the committee. Following the recent completion of the report, Garcia was told by FIFA that his findings would not be made public. Despite UEFA president Michel Platini's appeal for the report to be released and, if necessary, the 2022 World Cup vote to be repeated, Sepp Blatter has so far ignored calls for both greater transparency and a re-vote.
With this in mind perhaps the reason Qatar won the bidding process is actually much simpler.
Maybe it simply offered to provide enough material to allow for Blatter's heads to remain forever buried in the sand.
Discussion of the ethics of FIFA's sorry situation is miserable enough. It's the sanctioning of morally reprehensible action through FIFA's silence illustrates that FIFA's judgment is fatally flawed. Given the limited rights of women in the Gulf State and the treatment of homosexuals in both Russia and Qatar FIFA's dedication to tolerance and equality must be questioned.
To allow the suffering and deaths of so many employed in the name of the traditional sport of the working classes is also shameful. Workers on the sites of the World Cup stadiums talk of their passports being confiscated, leaving them stateless. Stranded and facing horrors if they attempt to escape, the migrant workers are forced to sleep in cramped and overcrowded living accommodation. That is if they survive the abominable safety standards of their workplace.
It is estimated that a staggering four thousand workers will lose their life working in Qatar before a single ball is kicked. Even more concerning, two British human rights investigators have disappeared in the past month while investigating Qatari construction sites.
If you take the decision to allow Russia and Qatar to hold their respective tournaments at face value it's easy to dismiss FIFA as woefully ignorant, bumbling even. But to do so is a very ill-judged idea indeed.
The reality is far darker and Sepp Blatter's inaction on issues of corruption and human rights abuses speaks volumes. This is the ugly side to the beautiful game and, frankly, nobody want to see it.

subset: commoncrawl


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How Much Does It Cost To Hire A Real…
Regardless of whether you're buying or selling, real estate is usually the most significant financial decision of your life, and one mistake could cost you tens of thousands of dollars and a lot of headaches. There's no wonder why people choose to trust real estate agents instead of trying to handle all the ins and outs themselves. But what is the actual cost to hire a real estate agent? Is it worth it? Maybe you're thinking that you could pocket a ton of cash by not hiring a licensed professional.
We get it! Everyone is looking for ways to save money. However, we think you might be disappointed at how little you will actually save if you skip on using an experienced pro to help you navigate the deal. Let's discuss all the value a good real estate agent provides, and then you can decide for yourself whether they are worth it or not.
Everything You Need To Know About The Cost To Hire A Real Estate Agent
Here's a list of everything you need to know about the cost of hiring a real estate agent and all the benefits that come with using one (especially on your first real estate transaction).
How Much Does a Real Estate Agent Cost?
The standard commission for a real estate transaction is typically 6% of the home's sale price. The commission is usually split between the seller's agent and buyer's agent, a 3% cut for each of them. For example, if you're buying or selling a $250,000 house, the agents would receive a total of $15,000 ($7,500 each). We get that it seems like a lot, so you want to make sure that you're getting what you pay for.
Keep reading as we break down that 6% and what that commission covers for your transaction.
What Does a Real Estate Agent Do?
A real estate commission covers all the work that goes into buying and selling property. There is a lot that goings into buying and selling a house. A seller's agent will help you stage your home to reach the right buyers, and most importantly, price it right. They take the time to get to know the area and compare prices so that it's most appealing to buyers and will still bring you a profit. They'll also make sure your home is seen by a ton of buyers, using a multiple listing service (MLS), social media, and ads. They have tools and tactics to get your home sold quickly and for top dollar.
On the other side, a buyer's agent studies home listings that meet your needs and are within your budget. They will arrange for a home inspection, negotiate for any necessary repairs or contract adjustments, and make sure you're getting a fair deal. Buyer's agents cover all of the ins and outs that go into finding you your dream home that you can afford.
Both seller's agents and buyer's agents can give you confidence knowing you have a real estate professional on your side. Plus, both agents share many duties:
- Meet with you in person or answer your phone calls whenever you have questions
- Give you access to an MLS to offer more options to buyers and more visibility for sellers
- Educate you on current market conditions
- Refer you to other professionals in the industry (inspectors, attorneys, mortgage lenders, photographers, and more!)
- Schedule showings
- Negotiate the best price and help you through mountains of paperwork
- Represent you and act in your best interest throughout the entire process, no matter how long it takes
A good agent tackles all of these tasks day in and day out. Their experience handling tough contracts and helping you avoid mistakes is well worth the cost.
We thought we should also mention that although commission and the closing costs are paid when a home sells, there are few other costs to consider. Home appraisal and home inspections are used to make sure that the house is worth the price and helps bring to light any problems in the home. Talk to your agent to get an estimate of how much each of those necessary services costs, so you have a better idea of what to expect.
Is Hiring an Agent Worth the Cost?
You know the cost, and the ins and outs of what goes on in the daily life of a real estate professional, now let's answer the question you're really asking – are real estate agents worth the cost? As we explained above, sellers typically cover the commission for both agents, so buyers have nothing to lose. Sellers, if you think you're getting the short end of the stick and are considering going the "For Sale by Owner" (FSBO) route, we want to lay out some stats.
FSBOs accounted for 8% of home sales in 2018. The typical FSBO home sold for $217,900 compared to $295,000 for agent-assisted home sales. That's a huge difference! Even if 6% of that goes toward agent commissions, that's still a chunk in your pocket that you would lost out on if you tried to sell yourself.
Make Sure You Find A Agent Who Knows Your Area
We hope this list has proved that a good agent is worth the cost of commission. The best piece of advice we can give you is to find an agent with plenty of experience. Your buddy who barely passed his exam might not be the best choice when it's your first home. Look for a pro who has a long history of success!
The team of experts at 41 Realty Group would love to be your go-to person when it comes to real estate. Buying, selling, investing – we have an agent in every area who would to help answer your questions, find you the perfect home, the perfect buyers, and more. Reach out to our team today and see if we can help you with all of your real estate needs.


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Mitochondria are multifunctional organelles that play a central role in energy metabolism. Owing to the life-essential functions of these organelles, mitochondrial content, quality and dynamics are tightly controlled. Across the species, highly conserved ATP-dependent proteases prevent malfunction of mitochondria through versatile activities. This study focuses on a molecular function of the plant mitochondrial inner membrane-embedded AAA protease (denoted i-AAA) FTSH4, providing its first bona fide substrate. Here, we report that the abundance of the Tim17-2 protein, an essential component of the TIM17:23 translocase (Tim17-2 together with Tim50 and Tim23), is directly controlled by the proteolytic activity of FTSH4. Plants that are lacking functional FTSH4 protease are characterized by significantly enhanced capacity of preprotein import through the TIM17:23-dependent pathway. Taken together, with the observation that FTSH4 prevents accumulation of Tim17-2, our data point towards the role of this i-AAA protease in the regulation of mitochondrial biogenesis in plants.
Mitochondria are essential, double membrane-bound organelles that are the main sites of ATP production in eukaryotic cells. Continuous degradation and biogenesis processes enable the maintenance of healthy mitochondrial populations and allow the mitochondrial proteome to adapt in response to the fluctuating cellular requirements (Nunnari and Suomalainen, 2012; Harbauer et al., 2014; Palikaras and Tavernarakis, 2014). The vast majority of mitochondrial proteins are nuclear encoded and synthetized as preproteins in the cytosol that are transported into destined sub-mitochondrial localization through the action of sophisticated, membrane-embedded protein translocation machineries (Wasilewski et al., 2016; Schulz et al., 2015).
Mitochondrial integrity considerably depends on balancing the levels of different subunits of protein complexes. This is achieved through various mechanisms, including selective removal of excess subunits by ATP-dependent proteolysis (König et al., 2016; Szczepanowska et al., 2016). Within the mitochondria, diverse ATP-dependent proteases with versatile activities are engaged in the maintenance of mitochondrial protein homeostasis (proteostasis) and function (Voos, 2013; Quirós et al., 2015; Janska et al., 2010).
The mitochondrial inner membrane-embedded AAA protease (denoted i-AAA) forms a homo-oligomeric ATP-dependent proteolytic complex that is embedded in the inner mitochondrial membrane (IMM) with the catalytic sites exposed to the intermembrane space (IMS) (Gerdes et al., 2012). In humans, a homozygous mutation in the gene encoding i-AAA protease (YME1L) was found to be associated with the development of mitochondriopathy with optic nerve atrophy (Hartmann et al., 2016). Upon loss or depletion of i-AAA protease, both in yeast and in mammals, pleiotropic phenotypes are observed. Yeast and mammalian i-AAA proteases display diverse activities, including proteolytic processing of mitophagy and fusion factors, selective elimination of misfolded and unassembled subunits, and control of the turnover of specific regulatory proteins that altogether influence a wide spectrum of processes occurring at the mitochondria (Thorsness et al., 1993; Weber et al., 1996; Nebauer et al., 2007; Ruan et al., 2013; Stiburek et al., 2012; Anand et al., 2014; Wang et al., 2013; Potting et al., 2010; Nakai et al., 1995).
Plant mitochondria contain two i-AAA proteases, FTSH4 and FTSH11 (Urantowka et al., 2005). Arabidopsis thaliana devoid of FTSH4 displays severe developmental and morphological changes under temperature-stress conditions (Dolzblasz et al., 2016; Smakowska et al., 2016). These alterations correlate with oxidative stress, imbalance in phospholipid metabolism, perturbation in respiratory chain complexes activity and aberrant mitochondrial morphology (Smakowska et al., 2016). Similarly, loss of FTSH4 affects development and leaf morphology at the late stage of rosette growth when subjected to a short-day photoperiod at optimum temperature (Gibala et al., 2009; Kolodziejczak et al., 2007). In contrast to yeast or mammalian systems, the endogenous substrates of i-AAA protease have not yet been described in plants and consequently, the molecular mechanisms detailing how FTSH4 maintains mitochondrial function remain elusive.
Here, we demonstrate that plant i-AAA protease FTSH4 is required for the turnover of the essential mitochondrial inner membrane preprotein translocase Tim17-2. The lack of functional FTSH4 protease is associated with an increased abundance of Tim17-2 and an enhanced import capacity of model substrates for the TIM17:23 translocase; Tim17-2 together with Tim50 and Tim23 form the core of the TIM17:23 complex (Wang et al. 2012). This study provides the first identification of the physiological substrate of plant i-AAA protease and links FTSH4 protease and mitochondrial preprotein import machinery.
Mitochondria devoid of FTSH4 accumulate Tim17-2
To understand the role of FTSH4 protease in the maintenance of mitochondrial function, we searched for primary changes caused by the loss of this i-AAA protease. Consequently, to diminish the risk of secondary effects, we sought to identify mitochondrial proteins that accumulate in ftsh4 mutants cultivated under optimal growth conditions, where no significant alterations in mitochondrial activity were observed (22°C with a long-day photoperiod, cycles of 8 h dark and 16 h light) (Smakowska et al., 2016). Interestingly, we found a substantial increase in the steady-state levels of Tim17-2 in mitochondria isolated from both ftsh4-1 and ftsh4-2 lines (Fig. 1; Fig. S1). Tim17-2 is a highly conserved, essential component of the inner mitochondrial membrane protein translocase – the TIM17:23 complex – that transports the majority of mitochondrial proteome (Wasilewski et al., 2016; Schulz et al., 2015). As evident from studies performed in yeast, Tim17 plays multiple roles within the TIM17:23 translocase. It regulates pore structure and voltage gating of the Tim23 channel, and is required for the promotion of the inner-membrane insertion of preproteins and direct interaction of the TIM17:23 core with the presequence translocase-associated motor (Martinez-Caballero et al., 2007; Chacińska et al., 2005). Consequently, the precise level and stoichiometry of Tim17 may be important for the optimal functioning of TIM17:23. A. thaliana contains three isoforms of Tim17, of which only Tim17-2 is essential and constitutively expressed (Murcha et al., 2014). We found that steady-state levels of other TIM17:23 subunits as well as levels of components of protein translocases from the outer mitochondrial membrane, Sam50 and Tom40, and of the intermembrane space-localized small Tim chaperone, Tim9, were unaffected upon FTSH4 loss (Fig. 1). These results show that the increase in Tim17-2 levels in ftsh4 mutants is specific and not caused by a general upregulation of mitochondrial preprotein import machineries. To further address correlation between the FTSH4 protease and Tim17-2 levels we generated a ftsh4-1 complementation line (ftsh4-1 FTSH4), in which FTSH4 was expressed under the constitutive CaMV 35S promotor (Fig. S2). Overproduction of FTSH4 rescued the morphological phenotype of ftsh4-1 line (Fig. S3) and led to a decrease in Tim17-2 steady-state levels (Fig. S2).
Next, we questioned whether Tim17-2 present in excessive amounts in ftsh4 mutants is correctly integrated into the mitochondrial membrane. To address this, we isolated mitochondria from wild-type and mutant plants and performed alkaline carbonate extraction that allows for distinguishing between integral membrane proteins (pellet) and soluble or peripheral membrane proteins (supernatant). Both in wild-type and ftsh4 mitochondria, Tim17-2 was exclusively found in pellet fractions after sodium carbonate treatment, indicating integration into the inner membrane (Fig. 2A). To confirm specificity of the assay, mtHsp70 (also known as mtHsc70-1, a soluble protein of mitochondrial matrix) and Slp1 (mitochondrial inner membrane protein that is partially extracted with sodium carbonate; Gehl et al., 2014) were used as controls.
We also addressed Tim17-2 distribution within mitochondrial membrane protein complexes by Blue native (BN)-PAGE followed by a second-dimension electrophoresis under denaturing conditions (2D-BN/SDS-PAGE) (Fig. 2B). In wild-type mitochondria, Tim17-2 mainly co-migrated with Tim50 as does the TIM17:23 translocase (Wang et al., 2012). A small portion of Tim17-2 in a low molecular mass sub-complex was detected. In contrast, a substantial fraction of Tim17-2 was present as a low molecular mass sub-complex in ftsh4 mutant. Furthermore, the ratio of TIM17:23 complexes in ftsh4 mutants was noticeably altered. Taken together, we conclude that FTSH4 controls the abundance of Tim17-2 in the mitochondrial membrane and that lack of this protease exerts an impact on the architecture of TIM17:23 translocase.
Proteolytic activity of FTSH4 is required to prevent accumulation of Tim17-2
Specific accumulation of Tim17-2 in ftsh4 mutant mitochondria strongly suggests that FTSH4 proteolytic activity is required for the turnover of this essential protein import component. In order to address whether Tim17-2 represents a proteolytic substrate of FTSH4, we generated a mutant line expressing a proteolytically inactive variant of FTSH4 [FTSH4(H486Y)] in the ftsh4-1 background. The overproduction of FTSH4(H486Y) was confirmed by immunoblot analysis (Fig. 3A). Expression of the proteolytically inactive variant of FTSH4 neither restored the morphological phenotype of ftsh4-1 line (Fig. S3) nor downregulated levels of Tim17-2 (Fig. 3A), demonstrating the importance of the proteolytic function of this i-AAA protease in vivo. To obtain further evidence for the role of FTSH4 proteolytic activity in the regulation of Tim17-2 levels, we analyzed turnover of Tim17-2 in isolated mitochondria. We observed a time-dependent decrease in Tim17-2 amounts in wild-type mitochondria, while the levels of this protein remained stable in mitochondria isolated from plants expressing proteolytically inactive FTSH4 (Fig. 3B). Moreover, we found that Tim17-2 specifically co-precipitates with FTSH4(H486Y) (Fig. 3C,D). We conclude from these studies that Tim17-2 represents a bona fide substrate of the FTSH4 protease.
Lack of FTSH4 protease is associated with enhanced capacity for the import of model substrates for the TIM17:23 translocase
An imbalance in levels of different complex subunits might lead to changes in the activity of the whole protein complex (König et al., 2016). Since our results suggest that FTSH4 protease controls Tim17-2 levels and by this modulates the architecture of TIM17:23 translocase, we questioned how the loss of FTSH4 influences mitochondrial protein import. To establish the role of FTSH4 in this process, we analyzed the capacity for in vitro uptake of the alternative oxidase (AOX, Genbank X68702), a precursor known to be imported via TIM17:23 translocase (Murcha et al., 2005), into ftsh4-1 mutant mitochondria (Fig. 4A). We found that mitochondria devoid of FTSH4 protease display a substantially increased rate of import for the radiolabeled AOX precursor. In contrast, in vitro uptake of the adenine nucleotide transporter (ANT) (Murcha et al., 2005), a precursor known to be imported via the TIM22 translocase complex, showed no difference in the rate of import in mitochondria isolated from ftsh4-1 compared to in wild type (Fig. 4B). Furthermore, we found that overexpression of FTSH4 protease in a ftsh4-1 background led not only to a reduction in Tim17-2 levels (Fig. S2), but also to a decrease in the rate of AOX import (Fig. S4). This is in line with previous findings, where the mammalian homolog of FTSH4, YME1L, was shown to downregulate the activity of TIM17:23 translocase by mediating the proteolysis of Tim17A isoform (Rainbolt et al., 2013). Increased import capacity through TIM17:23 translocase in ftsh4-1 mutant suggests that a low molecular mass Tim17-2 complex, which is present in elevated amounts in mutant mitochondria, could represent a functional translocase involved in import of preproteins. On the other hand, the TIM17:23 translocase is a very dynamic complex in which its subunits alter their conformations and undergo association and dissociation to facilitate preprotein import (Schulz et al., 2015). Therefore, we cannot rule out the possibility that the presence of excessive amounts of Tim17-2 induces changes in the architecture and dynamics of TIM17:23 translocase that have a stimulatory impact on import of at least a specific set of preproteins. Noteworthy, Tim17-2 contains a C-terminal extension that protrudes into the outer mitochondrial membrane providing a physical link between the inner and outer mitochondrial membranes (Murcha et al., 2005). Thus, accumulation of Tim17-2 in the ftsh4 mutant could enhance the tethering between mitochondrial membranes facilitating preprotein import through the TIM17:23 complex (Donzeau et al., 2000). However, the molecular mechanism detailing how the Tim17-2 levels control the rate of preprotein import into the plant mitochondria remains to be elucidated.
In conclusion, our report suggests that the FTSH4 protease has a novel role of in the regulation of preprotein influx into the mitochondria by regulating the abundance of Tim17-2 levels. These findings provide new insights into the significance of ATP-dependent proteolysis in the maintenance of mitochondrial proteostasis in plants.
A detailed list of reagents used in this study is provided in Table S1.
Plant material and growth conditions
Arabidopsis thaliana lines used were: ecotype Col-0 wild type, T-DNA insertion lines ftsh4-1 (SALK_035107/TAIR) and ftsh4-2 (GABI_103H09/TAIR) (Gibala et al., 2009), the ftsh4-1 FTSH4 complementation line and ftsh4-1 FTSH4(H486Y) mutant line, and were grown on 0.5× MS medium supplemented with 3% (w/v) sucrose in chambers in a 16 h light and 8 h dark (long-day photoperiod) at 22°C for 2 weeks with a light intensity of 150 μmol m−2 s−1. To test the morphological phenotype of A. thaliana lines, plants were grown in soil in a climate-controlled chamber in a 8 h light and 16 h dark (short-day photoperiod) at 22°C for 10 weeks.
Generation of ftsh4-1 FTSH4 complementation line
FTSH4 cDNA sequence was amplified using primers FTSH4.FLAGfor and FTSH4.FLAGrev (Table S2) and cloned into pENTR™/D-TOPO (Invitrogen). The resulting plasmid, FTSH4.FLAG pENTR, was used in a gateway LR recombination reaction with destination vector pGWB514, and a final construct, FTSH4.FLAG pGWB514, was obtained. The ftsh4-1 FTSH4 complementation line was made by the Agrobacterium tumefaciens-mediated transformation (Bernhardt et al., 2012).
Generation of the ftsh4-1 FTSH4(H486Y) mutant line
In order to abolish the proteolytic activity of FTSH4 protease, a highly conserved histidine at the position 486 (a Zn2+-binding site, in the HExxH motif) was mutated into a tyrosine residue (Westphal et al., 2012). The mutation was introduced on FTSH4.FLAG pENTR plasmid using a QuikChange Lightening site-directed mutagenesis kit (Agilent Technologies) and primers Mut_prot_H_FP and Mut_prot_H_RP (Table S2) resulting in a FTSH4(HIS).FLAG pENTR plasmid. Next, the Gateway LR recombinant reaction was performed with a FTSH4(HIS).FLAG pENTR plasmid and destination vector pGWB514. This resulted in the construction of a final plasmid, FTSH4(H486Y).FLAG pGWB514, bearing FTSH4(H486Y).FLAG under the constitutive CaMV 35S promotor. ftsh4-1 FTSH4(H486Y) line was made by the Agrobacterium tumefaciens-mediated transformation (Bernhardt et al., 2012).
Immunoblot analysis
Selected proteins were probed with the specific antibodies indicated in Table S3. Proteins were visualized with enhanced chemiluminescence (Mruk and Cheng, 2011) using a G-BOX ChemiXR5 (Syngene, UK) and the results were quantified with ImageJ software.
Isolation of mitochondria from Arabidopsis thaliana
Isolation of mitochondria from 14-day-old seedlings was performed accordingly to a well-established procedure (Murcha and Whelan, 2015).
2D-BN/SDS-PAGE analysis
Mitochondria were solubilized in digitonin-containing buffer and 2D-BN/SDS-PAGE analysis was performed as described previously (Wittig et al., 2006; Wang et al., 2012).
In vitro uptake of mitochondrial 35S-labeled precursor proteins
Radiolabeling of model mitochondrial preproteins (AOX; Genbank X68702) and ANT (Genbank X57556) with [35S] methionine and their import into mitochondria was performed as described previously (Duncan et al., 2015). Radiolabeled proteins were visualized by digital autoradiography (PharosFX Plus Systems, Bio-Rad) and analyzed with Quantity One software (Bio-Rad).
Carbonate extraction assay
To discriminate between membrane-integrated and soluble mitochondrial proteins, alkaline carbonate extraction assay was performed as described previously (Fujiki et al., 1982).
Analysis of the kinetics of protein degradation in isolated mitochondria
The kinetics of protein degradation in isolated mitochondria was analyzed by incubation of mitochondria in assay buffer (300 mM sucrose, 10 mM MOPS-KOH, 80 mM KCl, 5 mM MgCl2, 8 mM ATP, 12.5 µM ZnSO4 pH 7.2) at 35°, followed by SDS-PAGE and immunoblotting.
In order to perform immunoprecipitation of FLAG-tagged FTSH4(H486Y) protein, mitochondria were resuspended in digitonin solubilization buffer (1% digitonin, 20 mM Tris-HCl, 0.1 mM EDTA, 100 mM NaCl, 10% glycerol, pH 7.7) at 1 mg/ml. PMSF and EDTA-free protease inhibitor cocktail were added and samples were incubated for 30 min at 4°C with mixing. After a clarifying centrifugation (18,000 g for 15 min), solubilized material was loaded on anti-FLAG affinity matrix and incubated under constant rotation for 1.5 h at 4°C. After excessive washing steps proteins were eluted and subjected to SDS-PAGE and immunoblot analysis. Immunoprecipitation assays using antibodies raised against Tim17-2 and Protein-A–Sepharose resin were performed as described previously (Wang et al., 2012).
We thank Tsuyoshi Nakagawa (Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University, Japan) for vector pGWB514 and Lee Sweetlove (Department of Plant Sciences, University of Oxford, UK) for Slp1 antibodies.
Author contributions
M.O. and H.J. designed the study. M.O. and K.P. performed the experiments. M.W.M. provided the materials. All authors analyzed the data. M.O. wrote the manuscript with contribution from other authors.
This work was supported by a grant from the National Science Centre (Narodowe Centrum Nauki, NCN) (2015/16/S/NZ3/00364) awarded to M.O. M.W.M. is supported by an Australian Research Council Future Fellowship (FT13100112).
Competing interests
The authors declare no competing or financial interests.


text:
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text:
We received a sweet gift in the mail yesterday. It was addressed to "King Family of Eight" which was the first sign it would be awesome. Then upon opening, it was like my heart bursted. Taco Money to none other than Torchy's Tacos. I linked it for most of you to inform yourself about the amazingness that is Torchy's and then make a visit here or somewhere where there is one. Torchy's is what keeps our at home date nights thriving, our kids excited, and caters our end of week celebrations reminding us that we all still love each other… A LOT.
After a long day covered in spit up, milk, boogers, no shower, only serving left overs and saying "Sorry we will get groceries tomorrow" — Taco Money was the motivation I needed. It was the "You're doing so great you deserve more tacos" kind of LOVE that could move mountains. Speaking of food, my new nick names around the house are Snack Monster and Gobbler. You might find me eating the kid's food when they aren't looking or sneaking Drexel's homemade rice krispies at 4:00 a.m. I'm not worried about people knowing my true love for food because the scale is still going down with all that good nursing and my postpartum goals are simply to survive.
If you have been texting or calling me — I hope you know not to take it personal and most Moms seem to understand when I text you back several days later. I think I have currently responded to everyone, but all phone calls or discussions longer than 5 minutes are being routed through my Secretary/Bodyguard/Naptime Manager/HUBBY.
I don't exactly feel like I'm great at the newborn stage and I really wonder who in the world does feel that way. But Drexel says "You're doing great love." Regardless of what the truth is, I have been simply focusing on doing the next thing well. I don't know what the next thing looks like on any given day. One moment when I'm wide awake and showered I feel like I can take on the world. Then the next day I would prefer to sit and snuggle my baby/babies ALL day without interruption.
My brain can only get to the next thing and if you interrupt that "next thing" I was about to do, I will forget it. I wanted to wash my feet last night before bed. I stood in my bedroom standing there trying to remember what I was about to do next. Hubby threw out a few ideas of my usual bedtime routine, but none of them were it. I stood there staring off into space… "Wash my feet, ahh, yes…that was it." I washed my feet and crawled into bed.
Enough about me…let me tell you about the past 3 weeks with 16 hands and 16 feet.
It has been the most special postpartum time we have ever had as a family. It has been equally challenging, but any great thing requires hard work. I am incredibly thankful for my dear hubby who has made the transition to 6 kids so seamless. I mentioned the meals from friends, the gifts and all the encouragement in his birth story — it has all been very rich.
The kids have also been working hard and embracing their new roles. I have enjoyed special and intimate time with each one of them. Each child has said funny and entertaining things these past few weeks. I wish I wrote every single comment down. The toddler enjoys giving kisses {aka Mouth to Mouth}, the other toddler sings him to sleep, our daughter still calls him a She, and the oldest two correct me when I'm not quick enough to feed him or calm him down.
At times, it has been loud and slightly intimidating, but never have I doubted what God has done in our family. Although I may not know what the next thing looks like, I know this {and a whole lot more}. God continues to meet our EVERY need. My prayer these past few weeks has been Philippians 4:19. "And my God will supply every need of yours according to his riches in glory in Christ Jesus."
Most mornings and nights it has been a simple "Lord, show me what I need — meet my physical, emotional, spiritual and mental needs. Then meet my family's needs and help me meet their needs." I'm learning to entrust more of myself, my husband and our 6 kids to Jesus each and every moment. The day unfolds as it may and it is good to rest in this season of "One day at a time."
Our time of bittersweet postpartum bliss is coming to an end. I won't tell you exactly when just yet, but feel free to pray for me as I transition to full time Mom duties with these 6 little ones! Please pray for wisdom and discernment as I balance rest, work and play! I'm excited and definitely nervous, but mainly looking forward to how God equips me to do His special work.
With All My Love,
Thankful for these sweet pictures of Jedaiah at 5 days old. He is much chunkier now and I am hoping to get a family photo soon!


text:
Ars Technica reports on data from the International Federation of the Phonographic Industry (which sounds way more bad ass than the people who probably work there) predicting the majority of music revenue will come in the form of digital sales as early as next year. IFPI projects digital will generate over 52% of domestic music revenue in 2010 and globally by 2016. That's remarkable when you consider digital was just two percent of revenue when the IFPI started tracking the statistic in 2004, and only 36% in the US last year.
This should really shock many of you, as metal is still predominantly consumed as physical medium. Most metal bands see less than 15% of their sales come from digital.
Categorised in: Metal By Numbers


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It's just that Cumbria has got a new Bishop, ought I to dig out this old blog and send her a link to it?
Oh, before I start, a number of people have asked me about the six ewes scanned in lamb with triplets who have their legs crossed and are hanging on waiting for spring and some nice weather before lambing.
As of twenty minutes ago, they have all still got their legs crossed and are still hanging on.
But anyway now I've passed on the important news, is there anything else worth talking about?
Well somebody asked me about a joke about the Bishop and the Actress.
Now I was pondering this. One of the joys of the Church of England with its fine tradition of clerical eccentricity is that there are almost certainly bishops who've married actresses, and now after the latest round of reforms, give us a while and we'll have bishops who were actresses.
Well we've had bishops who'd been professional Cricketers, David Sheppard, Bishop of Liverpool was. He's the one who said something along the lines that, "When Saint Paul went anywhere; they stoned him and drove him out of town. When I visit they give me a nice cup of tea. I wonder if I'm doing it right."
So perhaps bishops are supposed to stir things up and cause trouble? I looked at the salary scale. Effectively if you're a bishop you're on a similar salary to a Civil Service Grade Eight (Senior Executive Officer.) This is about two thirds of a MPs salary, and you don't get their generous expenses. However you get a tied house to live in, but obviously you've still got to buy somewhere so you have somewhere to retire to.
The other advantage Bishops have is their background. If you want to find people who've done a variety of jobs in the 'real' world, you're probably better off looking in the House of Bishops than in the Cabinet or Shadow Cabinet. And as all Bishops have been ordinary clergy on less than the national average wage they're probably more in touch with the economics of the average household.
But is the purpose of a Bishop to say things that ensure people stone them? Perhaps it's a case that they ought to ask the questions that force other people to ask themselves questions? I'd suggest that if they're under attack by senior politicians and business leaders they're doing their job properly. Even if the bishop is wrong, they've still forced people to think about the question and produce a coherent justification.
But it also occurs to me that it's up to all of us to ask questions that make people stop and think. Some of the toughest questions are best made without a lot of fuss.
Some of the toughest are,
"Are the methods you want to use doing more damage than the problem you're attempting to solve?"
"How immoral are you entitled to be to restore morality?"
"At what point will your defence of freedom destroy the freedom you are trying to defend?"
Look, I'm currently a mixture of maternity nurse and lavatory attendant. Others might call me a farmer. When I turn my back on my proper jobs I write a bit.
A book should have a good story, it should make you want to turn the page, you should really enjoy it.
But when you finally put the book down and walk out to meet the day, I don't think it's a bad thing if, at the back of your mind, there's a small voice asking you a big question.
As a reviewer commented, "50 year old Benor is back in his home city of Toelar, enjoying a quiet life of roof running, paramouring, etc, when one day his routine gets disturbed, making a fast getaway necessary.
However, his escape route is blocked by an Urlan Knight.
Fortunately, the said Knight saves Benor's life, without even unsheathing his sword, by just being there.
Unfortunately, the said Knight has been looking for Benor and has a little proposition to make.
And so it begins…"
Tagged: auld witch, Justice 4.1, lambing, triplets, War 2.2


text:
Barber-Collins Security Services Ltd.
Barber-Collins Security understands how important it is to recognize the efforts of employees. Special programs have been implemented to celebrate employees who go above and beyond, stand out in excellence and have attributed to the positive reputation of the organization.
The "Shining Star" program recognizes and celebrates the outstanding work performance of individuals periodically throughout the year. This program is intended to share the achievements of extraordinary employees who have made smart choices, reacted in a manner that exceeds the expectation of the job and rewards team members who deserve to be recognized for a job well done. These employees can be nominated by a co-worker, a Supervisor, an Operations Manager, the Client and/or the general public.
The "Years Of Service" program recognizes employees who hit specific anniversary's within the organization. These milestones are acknowledged at 10 years, 15 years, 20 years, 25 years, 30 and 35 years of service. We celebrate your commitment and this program is a small way of showing you how much we appreciate your dedication.
The "United Way" program thanks employees who join us in supporting the community through bi-weekly/annual donations supporting the United Way. Each month a random draw is made to recognize qualifying employees and they receive a prize. In addition to this, their names are posted on this page and in the quarterly newsletter.
United Way Program Winners
The following names have been randomly selected using the names of all employees who have made contributions to the United Way Campaign for the current year. Thank you to everyone who participated and congratulations to the following winners:
(Prizes are delivered directly to your account by our Operations Team)
James Lowe, Duncan Quinn, Jacques Duguay, Richard Federer, Parmjit Kalsi, Pieter Mostert, Robert Ross, Ivanna Juricich and Puneet Banalore Nagaraj
Our 2018 Winners:
Fred Cepovski, William Ashwell, Gosham Massaquoi, Kyle Provenzano, Travis McApline, Bradley Fay, Connor Munro, Joanna Mills, Alan Price, Brynley Luckhai, Devon Smith and Richard Belanger
Thank you to everyone for your donations!
In Recognition of these Shining Star Moments!
"Upper Management has mentioned more than once how professional Lovedeep is. He knows his job, knows what he is supposed to do and how to react. In a few cases, his encounter with the Directors has been "on point" where I was told to commend him."
~ Larry Wallace & Management at the site Lovedeep is assigned ~
Badru is recognized as a Barber-Collins Shining Star
The following email was received from a member of the public regarding Badru
RE: Exceptional Service from Security Team
We have lived at (undisclosed location) for 2 years from May 2017 to May 2019. Over that time, we have been exceptionally well treated by the entire staff of the Condo, especially the Security Team.
We wish to especially mention Badru and Said who have always been very attentive, professional and helpful whenever needed.
We felt the need to express our gratitude for the excellent service.
Mrs. Olivia K. & Dr. Peter Q.
In recognition of a job well done and promoting the reputation of Barber-Collins Security, we would like to thank
Badru for always taking the initiative and for going above and beyond.
Thank you Barber-Collins Security
The following email was received from a former employee
First off, I would like to thank Barber Collins for my employment and the opportunity to work with the transit team, building relationships, improving my knowledge and working for an active, quick responding security company. I have worked for other companies in the past, and none of them were as quick, professional or prompt as BC is. From never having an issue with pay to quick replies from office staff.
Thank you
Damian Parmentier
Eric was contacted by the Office of the Chief of Police in April advising him that he was nominated for this prestigious award and that they were delighted to take this opportunity to recognize his actions which contribute to the safety of Waterloo Region. They wanted to recognize Eric's involvement in locating a suspected sexual offender.
Congratulations Eric!
We are very proud of your commitment to Barber-Collins and the members of our community.
This nomination came from a Client who wanted to recognize Ashok's response to a situation that occurred at their condominium.
While investigating a situation at their location, Ashok happened upon a victim who had been assaulted. He immediately contacted the police and ambulance. Once the police completed their investigation, they took the individual to the police station where she filed charges against the male suspect. The police complimented the Client for the video footage and the incident reports that were provided. They were particularly happy with the professionalism in which the matter was handled.
Congratulations Ashok! In recognition of a job well done, we would like to thank you for handling the situation professionally and for your assistance in this matter. We could not agree with our Client more when they stated "We are proud to have you on our team"
This nomination came from a Client who wanted to recognize the outstanding efforts of the above mentioned employees.
"Thank you to you and your team for another successful swearing in ceremony yesterday. The feedback provided was that the team was very courteous, professional and kind. Your team does behind the scenes work to make the event run smoothly and troubleshoot any last minute concerns. We are so fortunate to have a great relationship with you and we truly appreciate all that you do going above and beyond for CSD."
In recognition of a job well done and promoting the reputation of Barber-Collins Security, we would like to thank each of you for your excellent service and for going above and beyond!
Your nomination comes from an account associate who wanted Barber-Collins to recognize her efforts.
"As I could not find Brandy's name on the accounts recognition hub, I would like to recognize her for an outstanding client service and for inspiring through example. Whenever I visit the head office location, one of the things I look forward to is seeing Brandy and saying hello. Brandy is very responsive and is always helpful to everyone. Some of my visit is due to third party visitors coming for a meeting and I have observed the way that Brandy attends to guests and staff alike; it is always in a very courteous and professional manner. Thanks Brandy! For your service"
Thank you Brandy for your hard work and dedication. Your professional and positive behaviour has not gone unnoticed and we join this associate in congratulating you for your Shining Star attitude.
This nomination comes from a co-worker who wanted Barber-Collins to recognize Philips efforts.
"I just wanted to extend my appreciation for the work of Philip today. During an unusually busy time at our assigned location, including 2 active arrests, and an ongoing fight, Philip was able to conduct himself calmly and without delay to all of the requests made by our units over the radio, while also assisting in notifying Waterloo Regional Police and monitoring the guards on CCTV to ensure their safety."
We join Mr. Lean in thanking you for your assistance and we pass along the team's gratitude for the help.
Our office was contacted by a professor at Conestoga College. She had great things to say about two employees who volunteered as part of their Careers Course. This is the amazing email we received from her.
"I recently had two Conestoga College grads, Erik and Patrick, in as guest speakers for the careers course. They both work for you at the Grand River Transit stations. They presented in such a professional manner, both were well-spoken and left such a positive impression of Barber-Collins. I wanted to let you know how well some of your employees, these two in particular, represent your organization. They were wonderful with the students and stayed afterwards to chat with those interested."
Congratulations Erik and Patrick! By contributing your time to the community, promoting your jobs in security and presenting yourselves in such a professional manner, you have made our organization proud!
"Character is defined by what we do when we think no one is looking"
Barber-Collins received an email from a grateful Mother, who wanted to express her heartfelt gratitude and appreciation for the actions taken by Tristan when he was heading home after work. Tristan happened upon her daughter in a situation that required medical attention. Not only did Tristan contact emergency responders, he provided comfort while they waited for the ambulance. In addition to waiting, Tristan took it upon himself to contact the young girls mother to advise her of the situation. When the mother showed up at the scene, Tristan was very humble when asked for his personal information and the only real identifier was his name tag on his uniform. The daughter was taken to the hospital and the following day received flowers from Tristan who sent her well wishes.
Congratulations go to Tristan for going above and beyond, recognizing an emergency situation, taking on the role of first responder until EMS arrived and for reaching out to the young ladies Mother to apprise her of the situation. You are a poster child for our company mission-providing impeccable service (on and off the job), treating people with respect and dignity and doing your part to helping someone in need.
We are very proud of you!
One of the greatest compliments Barber-Collins can receive is positive feedback. The following email was received from a patron at one of our Client locations.
"Good Morning, I just wanted to extend a thank you to you for how you helped me yesterday. My dad had dropped a key off in an envelope with the front desk on Tuesday. I was in a rush and had to pick it up the next morning. When I walked up to the counter, you somehow knew that it was for me and asked "Lindsey?" before I could even tell you what I was looking for! Your attention to detail and ability to recognize this has me in awe, thank you for all you do! You are awesome!"
Congratulations Bob! Making our Clients feel valued is one of the best compliments we could pay them!
These employees were selected as the Shining Star by several tentants of a senior residence we were providing short term coverage while elevator maintenance was being performed.
Our office was contacted by several tenants of this location advising us that our staff behaved exceptionally, helping tenants with groceries, walkers and up and down stairs. They wanted to share that our staff treated them with kindness, respect and dignity. Each email that our office received communicated the same message, they wanted to thank us for sending qualified and kind men.
To this we say, thank you to each of you for a job well done and for promoting the reputation of Barber-Collins Security. Thank you for taking the initiative, for your kindness and for going above and beyond.
Damian was selected as the Shining Star for May by his Site Supervisor Aaron Bak and staff members working at the security site Damian was assigned.
On May 24th, 2016, Damian responded to a person in crisis who was disturbing the peace. Damian arrived on site and informed the staff that he would take care of the situation which resulted in contacting the WRPS and assisting them in apprehending the subject. By communicating his intent to the site staff, they were assured the situation would be taken care of and this gave them peace of mind. The staff relayed to the Client that they were extremely happy with his response and conduct.
Your actions and support have not gone unnoticed and we want to thank you for going above and beyond and providing exemplary service! Great Job Damian!
Humair was selected as the Shining Star for May by Operations Manager Jerry Collins.
While monitoring surveillance CCTV system on site, Humair witnessed a child's hand slide and become stuck in the door of an elevator at the condominium he is assigned. We are told that he responded by jumping over a counter, racing down a hallway, a flight of stairs and back up a hallway to reach the elevator. Having viewed the video footage, we noted that his response time was 34 SECONDS! We are happy to share that the child is fine and thanks to Humair's keen eye and excellent response time, was released from this dangerous situation within seconds.
Congratulations and Thank you Humair for going above and beyond and for being an excellent example of an employee who responds well under pressure. Great job!!
Tim was selected as the Shining Star for April 2016
The following email was received from an employee at the account where Tim was working.
My name is Annette, I am an Operations Supervisor. I am writing you today to pay a compliment to one of your employees. He is one of the security guards working at (undisclosed location). His name is Tim Goode.
This morning I witnessed him (Tim) dealing with an extremely irate patron. I have to say he handled the situation with grace and professionalism. He remained calm and respectful to the patron the entire interaction even though the man was yelling at him swearing at him to the point where the patron became aggressive kicking his belonging around.
Please pass on my observation to Tim Goode. "Job Well Done!"
In recognition of a job well done and promoting the reputation of Barber-Collins Security, we would like to thank Tim for diffusing a volitile situation by using a solid set of communication skills, remaining professional, calm and respectful.
Great Work!!!
Steve was selected as the Shining Star for January 2016
The following email was received from a member of the public regarding Steve.
"I was coming in for an overtime shift on Dec 11 at 4:30am. I had to call Security to let them know I would be entering defined location at that hour of the morning. Crazy as it is.
Unknown to me Security was going to be on site because of overnight contractors that were there. When I spoke to Steve he asked that I check in with him when I was in the building. I agreed to that but did say to him I would be coming from the parkade and if I don't show up by 4:30 come looking for me. This was said jokingly but serious as well because of the early time of morning and not sure about the area being uptown.
Here is where Steve went above and beyond. To my surprise when I came out of the parkade. He was waiting on the corner of the road outside of the parkade. I said are you Steve he said you must be Suzanne. We had a pleasant conversation and I thanked him for caring. He said it was no bother and he wanted to make sure I felt safe. I believe he said he thought I sounded unsure of the parking.
Typing this gives me chills and tears. He really is a credit to the security team we have here. Showing people matter. He didn't have to come meet me. He obviously knows people and can pick up on verbal cues very well.
I just want it noted that his 'good deed' did not go unrecognized.
And to many people I have shared this with both professionals and law and security persons they all agree.. He is a credit to his job. Thanks for your time"
In recognition of a job well done and promoting the reputation of Barber-Collins Security, we would like to thank
Steve for taking the initiative and for going above and beyond.
Jeff was selected as the Shining Star for October 2015
The following email was received from a member of the public regarding Jeff.
I had an amazing experience today with a staff member of the airport today and I was hoping to get his name so that I could write him a letter and personally thank him.
My mom thought she had a flight with American Airline today at 3:20 pm. I had lunch with her and left her in the airport alone. She found out soon enough that her sister who lives in Texas had confused the flights and it had departed at 7 am. My mom was absolutely devastated. The security guard who was working alone in the airport was absolutely phenomenal with her. He was so patient and understanding and calm and helpful.
I called to thank him but hours later I still can't stop thinking about how simply amazing he was. It is very rare these days you find someone who goes above and beyond.
In recognition of a job well done and promoting the reputation of Barber-Collins Security, we would like to thank
Jeff for taking the initiative and for going above and beyond.
Douglas was selected as the Shining Star for September 2015 by Operations Manager Mike Cook
Mike stated that in August, early evening, at one of our high profile security locations Douglas was monitoring the CCTV cameras. He observed a vehicle enter the lot. The vehicle fit the description of a vehicle involved in a theft from the property 2 days before. The vehicle was observed driving to trailers that were in the lot. The previous theft had involved a theft of property from a trailer. Police were called. Police arrived catching the culprit in the process of breaking into a trailer. The culprit was arrested and is being processed for the previous theft as well. We always like to catch the bad guy.
In recognition of a job well done, we would like to thank Douglas for his swift and professional reaction and for being a key member of our security team.
The following email was received from a member of the public regarding Quintana in August 2015
On Sunday night my wife and I got lost in the Kitchener/Waterloo area, trying to find the Princess Theatre. I entered a parking lot to turn around and try again. One of your vehicles was just pulling out. I jumped out and flagged it down. I explained my predicament to the driver, and she tried several times to direct me. Finally, my deepening deer-in-the-headlights expression probably helped her to suggest another alternative. "Follow me", she said, and led us for probably only about half a kilometer, right to the door, and saved a rare date night.
I cannot imagine that what she did was in her job description, or ever to be approved of as a general practice, but I want you to know that there is an employee out there, who, apart from knowing the area very well, is polite, helpful, friendly, people oriented, professional, and in every way a positive advert for your services. When I asked, she said everyone called her "Kee", at least that is what it sounded like.
Everything else aside, the episode raised the profile of Barber Collins for at least one member of the public.
Yours sincerely, Jock Tolmay
In recognition of a job well done and promoting the reputation of Barber-Collins Security, we would like to thank Quintana for taking the initiative and for going above and beyond.


text:
U.S. users downplay cellphone cancer warning
NEW YORK | News last week that an arm of the World Health Organization said cellphones might raise the risk of brain cancer has been greeted by Americans mostly with a shrug of the shoulder — one that's pinning a cellphone to the ear.
Google searches for "cancer" and "cellphones" spiked this week. And some people vowed to get headsets to shield themselves from radiation. But most seemed to either dismiss the warning as too vague, or reason that if the most useful device in modern life poses a slight health risk, then so be it.
"I was watching the news about it, and I thought, 'I'm already screwed because I've been talking on the phone for seven years,' " said Genevieve Chamorro, a 31-year-old New Yorker who was shopping for a phone.
John Gottani, a manager at a cellphone store in New York, said he's been selling phones for six years and has never heard anyone ask if they cause cancer. The only things customers really care about, Gottani said, are "if it works and if it texts."
The International Agency for Research on Cancer reviewed dozens of published studies on cellphones and cancer before classifying cellphones as "possibly carcinogenic" on Tuesday. It's a risk category that includes night-shift work, engine exhaust and coffee.
Studies haven't been able to rule out a link between cellphones and cancer. But experts say that if there is a link, it's unlikely to be strong. Cellphones emit weak radio waves, which, under the conventional understanding of physics, can't wreak the same sort of cellular changes that sunlight and radioactivity can.
A common tip offered to those who want to reduce their exposure to cellphone radiation is to use a headset. Even wireless Bluetooth headsets reduce radiation exposure. Though they emit radio signals of their own, they're much weaker than cellphone signals.
But there seems to be little rush to get Bluetooth headsets. They've been declining in popularity for at least four years, according to research firm Strategy Analytics. It's also found that most headset owners don't intend to replace the one they have when it wears out.
Cellphones differ in how much radiation they emit. Proposals in a few states would force cellphone stores to display these radiation ratings.
But CTIA-The Wireless Association, the cellphone industry trade group, is fighting these moves. It says there's no evidence the measured ratings have any correlation with risks. And cellphone manufacturers and carriers are showing no sign of breaking ranks with each other to use the ratings to their advantage — for instance, by touting "low-radiation phones."
Spokesman John Walls said CTIA wouldn't fight a manufacturer that wanted to market a "low-radiation phone." But claiming a phone to be safer than any other would cross the line, he said.
"They're all deemed safe by science," Walls said.
Americans on average talk about 700 minutes a month on their cellphones, making them some of the most talkative people in the world, well ahead of Europeans.


text:
Whatever you think about The Horrors' own music (and some people really hate it, or at least pretend to to make a point), you've got to admit they have impeccable taste in the music they like. Group A exist at a rawer, more avant-garde location in roughly the same postpunk/kraut/industrial taste spectrum that The Horrors seem to occupy, so despite the obvious differences in how the two groups sound, the sensibility that they share ensures that shifting things a little bit one way or another maintains a sort of thematic consistency.
Group A's original Initiation is a stark, metallic, minimal Throbbing Gristle pastiche, but in this remix, Tom Furse of The Horrors softens that somewhat, making the vocals clearer and adding a bouncy, if still menacing, synth bass. To say that this remix is poppier than the original says more about Group A's own version than it does about Furse's take on the track, which is thicker but still functions as a relentless early 80s-style synth-EBM drone. With her voice emerging from the back of the cavern of reverb that it had previously occupied, Tommi is here present as a sort of sneering cockney punk. The overall function is to bring the song up closer to the listener, more insistent than insidious, but also with an obvious understanding of and respect for what the original was trying to achieve.
The track will be available to download for free for a few more days and there's apparently more to come, so keep an eye out.


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See What David Harbour Could Look Like As Black Widow's Red Guardian
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Although Black Widow, the first installment of the Marvel Cinematic Universe's Phase 4, wrapped up filming early last month, official footage from the movie still hasn't been released to the public. As such, we don't have any good looks at Black Widow's new characters, including David Harbour's Red Guardian, but one artist has put together a solid mock-up of what the Stranger Things star could look like as the Russian super soldier.
Okay, technically speaking, we've gotten one look at David Harbour as Red Guardian thanks to a Black Widow poster that was on display at D23, but that was a profile shot where he was presented alongside a bunch of other characters. We saw the side of his mask and the star emblazoned on his chest, but that's it.
This artwork, made by Instagram user britedit, provides a much better idea of what David Harbour will look like in the Red Guardian costume, although to be clear, this is not official in any way. Still, working off the tiny portion of the Red Guardian suit we have seen, plus taking into account the character's ties to Captain America, it's a logical guess on how he could look in action during Black Widow.
All we know about David Harbour's Red Guardian is that his real name is Alexei Shostakov (this was the second individual to hold the Red Guardian mantle in the comics (opens in new tab)), he's the Russian counterpart to Captain America (we have yet to learn how he received his special abilities) and he has history with Natasha Romanoff. Whether they'll be allies for the whole movie or if Red Guardian will be revealed to have sinister intentions remains to be seen.
With six months to go until Black Widow's release, we still don't know any official plot details other than it will follow Natasha Romanoff alone and forced to confront her past between the events of Captain America: Civil War and Avengers: Infinity War. And she won't be the movie's only Black Widow, as Florence Pugh's Yelena Belova and Rachel Weisz's Melina also go by that moniker.
Joining all of the previously mentioned actors are O-T Fagbenle, Ray Winstone, Olivier Ritchers and a yet-to-be-identified actor who's playing Taskmaster. We'll also see William Hurt and Robert Downey Jr. reprise Thaddeus "Thunderbolt" Ross and Tony Stark, respectively, although Downey's appearance will be through unused footage from Captain America: Civil War.
Black Widow opens in theaters on May 1, so keep checking back with CinemaBlend for continuing coverage, including when the first trailer finally drops. In the meantime, you can learn what other MCU movies are slated for Phase 4 and beyond with our comprehensive guide.
As for David Harbour, he's also set to appear in Dhaka, which doesn't have a release date yet. The fate of his Stranger Things character, Jim Hopper, still hasn't been revealed yet, so we can't say for sure just yet if he'll appear in Season 4.
Connoisseur of Marvel, DC, Star Wars, John Wick, MonsterVerse and Doctor Who lore. He's aware he looks like Harry Potter and Clark Kent.
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Supreme Court Judgments
Rani Vs. M.K.Girish [2009] INSC 1240 (20 July 2009)
APPELLATE JURISDICTION CIVIL APPEAL NO.4558 OF 2009 (Arising out of S.L.P. (C)
No.17452 of 2008) B.V. Smitha Rani ...Appellant(s) Versus M.K. Girish
...Respondent(s) O R D E R Leave granted.
learned counsel for the appellant.
In spite
of service of notice, nobody has entered appearance on behalf of the respondent
to contest the prayer made in this appeal.
parties were married on 28.5.2003. After sometime, the appellant- wife filed
Matrimonial Case No.82/2005 for grant of divorce on the ground of cruelty. In
support of her case, the appellant examined herself as PW-1 on 7.9.2006.
On that
day, the respondent-husband remained absent and his lawyer did not cross-
examine the appellant. Thereafter, the case was posted for evidence of the
respondent on 28.9.2006. At that stage, the respondent applied for recording
his evidence by video conferencing. The Family Court rejected his prayer and
the special leave petition filed by him was dismissed by this Court. On
28.9.2006, the respondent's counsel filed vakalatnama and written statement.
The Family Court rejected the written statement on the ground of undue delay.
Thereupon, the respondent filed writ petition no.1031 of 2006, which was ...2/-
-2- allowed by the High Court and a direction was issued to the Family Court to
allow the respondent to contest the case on merits.
furtherance of the High Court's directive, the Family Court fixed the next date
as 7.11.2006 for cross-examination of the appellant, but the respondent did not
avail that opportunity. On the next two dates also, the respondent's counsel
did not cross-examine the appellant. Instead, an application was filed on
behalf of the respondent for amendment of the written statement, which was
dismissed on 11.1.2007. Simultaneously, the Family Court closed the evidence of
the respondent and fixed the case for arguments. Application filed on behalf of
the respondent for recall of that order was dismissed on 23.1.2007 and the
petition filed by the appellant was decreed on 22.2.2007.
On an
appeal preferred by the respondent, the High Court remanded the case to the
Family Court with the direction to give final opportunity to cross- examine the
appellant (PW-1) and to adduce his evidence. That order has been challenged in
this appeal.
heard learned counsel for the appellant and perused the records, we are
convinced that the High Court was not justified in remitting the matter to the
Family Court with a direction to give final opportunity to the respondent to
cross-examine the appellant and to adduce his evidence. The premise on which
the High Court passed the impugned order, namely, non-grant of adequate
opportunity to the respondent to cross-examine the appellant and adduce his
evidence is clearly erroneous, because, as mentioned above, after disposal of
writ petition no.
filed by the respondent, the Family Court fixed the case on three ...3/- -3-
different dates for cross-examination of the appellant, but the respondent did
not avail that opportunity. In this view of the matter, the Family Court had
rightly closed the evidence of the respondent, heard the arguments and
pronounced the judgment and the High Court committed serious error by remitting
the matter for giving further opportunity to the respondent to cross-examine
the appellant and adduce his evidence.
the appeal is allowed, impugned order rendered by the High Court is set aside
and the matter is remanded to it to dispose of the appeal on merits in
accordance with law after giving opportunity of hearing to the parties.
New Delhi,
July 20, 2009.
Pages: 1 2 3


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Kabir Singh (2019) is the best remake of a South Indian movie Arjun Reddy (2017). This Kabir Singh movie is one of the best remakes ever seen in Bollywood.
Kabir Singh movie ratings
Times of India movie rating: 3.5/5
Kabir Singh Movie Overview
Kabir Singh Movie Trailer
Kabir Singh Movie Storyline
Kabir Singh Movie Songs
- Bekhayali song
- Tujhe Kitna Chahne Lage song
- Tera Ban Jaunga song
- Mere Sohneya song
- Kaise Hua song
- Yeh Aaina song
- Pehla Pyaar song
Kabir Singh Movie Box office success
Kabir Singh Movie Critic reviews
The Shahid Kapoor Kiara Advani starrer KABIR SINGH is a wellmade love saga with tremendous appeal for youth and will surely bring audiences in hordes to cinemas.
by Bollywood Hungama | Full review at Bollywood Hungama
For those who have watched Arjun Reddy, Shahid Kapoor's Kabir Singh might not completely rip your heart out. But, it will definitely leave you with a heartache instead.
by Madhuri V | Full review at Filmibeat
While Kabir Singh is a welcome change from stereotypical love stories, this kind of love affair needs some getting used to.
by Ronak Kotecha | Full review at Times of India
Shahid Kapoor stretches himself very thin trying to convince us that Kabir Singh is a present-day incarnation of Devdas.
by Saibal Chatterjee | Full review at NDTV
Kabir Singh is engaging, a faithful remake that works well for those who haven't seen the original.
by Behindwoods | Full review at Behindwoods
Sandeep Vangas Kabir Singh starring Shahid Kapoor and Kiara Advani is raw, rustic and realistic. It is not a stereotypical Bollywood romance drama.
by Sify Movies | Full review at Sify Movies
Shahid Kapoor shines in this one.
by Priyanka Sinha Jha | Full review at News 18
Kabir Singh Movie Audience reviews
Kabir Singh is the best remake of a South Indian movie after Wanted (2009) starring Salman Khan and Ayesha Takia.
by Aswathi Prasobhan
It's a wow movie with an average old concept plot put in modern college lovers with adultery in words, dialogues and humor.
by Rikky Bhartia
Kabir Singh: This was one movie i was dying to see ever since i fell in love with its songs and have all of them in a loop on my play list
by Rakhi Sharma
Love with obsession!This is what is Kabir Singh is all about.This one is a fantastic remakes I have ever seen.
by Ayesha Chatterjee
Older Shahid is looking more handsome than younger Shahid😄. The more he aged, the hotter he gets.😍
by Jasmine Jongte
One of the Best part of movie is It doesn't have any remake songs.
by Gaurav Vashisth
There is no damn freaking actor in this whole industry who would have done this role better than Shahid.
by Shaun Capital
Shahid Kapoor deserves 65th Filmfare for best actor of the year. One and only.
by Kafika
Shahid Kapoor ke face par saare emotions clear dikhte hai…. Great performance 👏👏👏
by Udit Kumar
Bhai priti jaise gf ho na ho farak nhi padega but shiva jaisa dost sabko hona chahiye . Jisne movie dekhi use hi samjh aayega ye..😍
by Sagar Bhaskar
Just saw Kabir Singh. What a Mindblowing film. Inspite of all the controversies, it is an intense love story at its heart and I absolutely fell in love with the character of Kabir Singh.
by Priya Saraiya
After seeing Arjun Reddy, seeing Kabir Singh is nothing but reliving the experience in Hindi, a language closer for comfort.
by Nishant Menon
It is one of the most beautiful story that is intensifying due to Shahid's histrionics in the right measure.
by Bhaskar Sharma


text:
When it comes to the coronavirus, Naomi Campbell isn't taking any chances.
The supermodel, 49, geared up for a plane ride in a full-on hazmat suit. Underneath, she sported safety goggles and a face mask. "On the move…," Campbell captioned an Instagram post documenting her experience. It was unclear based on the star's Instagram post where she was jetting off to.
In a March 10 Instagram post, the A-lister shared another image of herself clad in the hazmat suit, teamed with pink gloves and a teal face mask. She completed the outfit with black sneakers.
According to Johns Hopkins, the coronavirus has infected more than 4.7 million people worldwide as of Monday morning ET, leaving over 315,000 dead. In Campbell's native United Kingdom, there have been about 245,000 cases of the coronavirus recorded, along with over 34,700 deaths.
Even prior to the virus, Campbell had put an intense airport routine into place to prevent herself from contracting illness. In an "Airport Routine" video shared to the "Making the Cut" judge's YouTube channel last year, she puts a seat cover over her chair, wipes down every visible surface (including the tray table, TV, window and overhead controls) and puts on a sanitary face mask. Campbell explained that she goes through this process regardless of whether she is flying on a private or commercial flight.
"As the plane descends, people start coughing and sneezing. I just can't," she said. "This is my protection for people coughing and sneezing."
Click through the gallery to see Naomi Campbell's Met Gala red carpet style through the years.


text:
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text:
Margaret R. Junior, a longtime resident of New Rochelle, died peacefully on August 28, 2019 surrounded by her family. She was 82.
Margaret, known as "Aunt Marge" to many, was born on December 18, 1936 in Glasgow, Scotland to the late William R. and Annabella (Baird) Kennedy. She came to the United States November 2, 1957, and went to work a week later for New York Telephone Company where she worked for over 42 years. During her time there she was an active member of the Pioneers Club.
As a devoted mother and grandmother, she always felt fortunate to be able to spend so much time with her children and grandchildren and be such a big part of their lives.
Margaret was known by many for her kind, caring heart, her boundless energy, her commitment to help and care for others, and her passion to support numerous charities. For more than ten years she served as President and Treasurer of the Tenants Committee at 35 Maple Avenue where she organized and held regular monthly luncheons for the tenant community. A great passion of hers was knitting and crocheting. She was especially known for her beautiful blankets, scarves and hats, and Christmas stockings. It gave her great joy to knit or crochet these special pieces for her family and friends.
Two of her other passions were shopping and travel. On any given day of the week one could find her browsing and shopping the aisles in her favorite stores. When she went on holiday to visit family and friends in Scotland she always referred to going back "hame". She was proud of and always kept true to her Scottish heritage. As a true Scot, Margaret always enjoyed a wee cup of tea. Those close to her were certain to have learned how to make a good cup of tea.
She was predeceased by her husband Thomas M. Junior who died on September 29, 1998.
She is survived by her son Thomas A. (Liz) Junior; her daughter Linda M. (Jim) Brown; her 4 grandchildren: John, William, Tommy and Michael; her brother William Kennedy; her sister Rita (Joe) Blondo; her nephews and niece; her cousins and many friends.
Margaret was a devout catholic who had unending faith in God and a longtime member of Blessed Sacrament Church. She will be dearly missed by her family and friends.
Visiting hours will be Wednesday 1-4 & 6-9pm at Lloyd Maxcy & Sons Beauchamp Chapel, 16 Shea Place, New Rochelle. A funeral Mass is scheduled for Thursday 10:30am at Blessed Sacrament Church. Interment will follow at Beechwoods Cemetery.
In lieu of flowers, donations may be made in her memory to:
St. Jude Children's Research Hospital
Rosary Hill Home
600 Linda Avenue
Hawthorne, NY 10532


text:
Retired UGA professor Judith Ortiz Cofer dies; remembered for literary talent and teaching
Judith Ortiz Cofer, a long-time University of Georgia professor of English and an accomplished poet and author, died Dec. 30 at her home in Jefferson County. She was 64.
Cofer, who in 2010 was inducted into the Georgia Writer's Hall of Fame, was a professor from 1984 until she retired in 2013. She became ill from cancer not long after retirement, but continued to make speaking engagements, including a literary festival last April at Western Carolina University.
Cofer, who died at her home near Louisville, was buried in the Louisville City Cemetery. While a professor at UGA, she lived in Athens, then spent weekends at her home with her husband, John Cofer, a high school mathematics teacher. She had one daughter.
A native of Puerto Rico, her family moved to New Jersey when she was a child and in 1967 they moved to Augusta.
"She was a wonderful writer of essays, poetry and novels," said UGA English Professor Hugh Ruppersburg. "One of the main things in her works was the experience of moving to the United States from Puerto Rico as a young child and adapting herself to becoming accustomed to another culture."
"She was a great teacher," said Ruppersburg, who befriended her not long after she joined the English Department. "She worked hard to help her students be successful and she stayed in touch with them after they graduated. I think that the loss of her as a human being and personality is a terrible lost."
"Judith Cofer was one of the wonderful poets of her generation. She was witty, philosophically deep and emotionally touching," said Betty Jean Craige, also a retired professor from the English Department and a longtime friend.
"She was a beautiful representative of the Latino community, but she was a poet for everybody," Craige said. "She was nationally known and widely anthologized for her poetry from the perspective of Hispanic Americans, but the appeal of her poetry was not limited to Hispanic Americans."
When Cofer was working on her first novel, she allowed her husband and Craige to read each chapter as it was finished, Craige recalled.
"She was my favorite living poet and Dylan Thomas (1914-53) was my favorite dead poet. Now she will take her place alongside Dylan Thomas," Craige said about her friend.
Among Cofer's works were "The Line of the Sun," a novel that was nominated for a Pulitzer Prize, and she won an O. Henry Prize for the story "A Latin Deli." Cofer's works are also in English literature text books for high school students.
In a 2003 interview with the Athens Banner-Herald, Cofer said, "'The best advice I can give is to be engaged in an activity that gives meaning to your life. When you get up in the morning, you say this is what I want to be engaged in doing. My husband loves teaching high school mathematics and seeing the results in the classroom. My daughter is a research mathematician and she also teaches. They have a life's work as opposed to an occupation. And that's how I see my writing, as my life's work."


text:
Scientists and industry partners are field testing a vaccine against porcine epidemic diarrhea virus (PEDV) in Saskatchewan, as well as in Manitoba where it is being used to help protect piglets from a recent PEDV outbreak.
The vaccine was developed at the University of Saskatchewan Vaccine and Infectious Disease Organization-International Vaccine Centre (VIDO-InterVac).
"Our new facility, InterVac, provided us with the containment infrastructure to develop a vaccine and demonstrate it protected up to 100 per cent of the piglets," said Volker Gerdts, PhD, VIDO-InterVac's research director, in a press release.
Animal health industry partnership
The successful vaccine results triggered the interest of several animal health companies including Huvepharma, which has partnered with VIDO-InterVac to develop the technology for commercial production in North America.
"Our goal is to have the vaccine available for commercial use as soon as possible to help stop producer losses," said Dr. Boris Gavrilov, senior scientist for biologics development at Huvepharma.
PEDV background
PEDV hit the United States in 2013 and spread to Canada in 2014. It was first discovered in Europe, and has become increasingly problematic in Asian countries.
Occurring only in pigs, PEDV can kill up to 100 per cent of infected piglets. PEDV is a coronavirus, a virus group which includes important emerging human diseases such as SARS and MERS.


text:
The board seeks to provide all students with the opportunity to complete graduation requirements without discrimination and without disparate impact on groups of students. In so doing, the board acknowledges that circumstances may arise that prevent a student from earning all thirty credits required for high school graduation. Such circumstances may include, but are not limited to, the following:
- Homelessness.
- A health condition resulting in an inability to attend class.
- Limited English proficiency.
- Disability, regardless of whether the student has an individualized education program or a plan under Section 504 of the federal Rehabilitation Act of 1973.
- Denial of an opportunity to retake classes or enroll in remedial classes free of charge during the first four years of high school.
- Transfer during the last two years of high school from a school with different graduation requirements; and
- Other circumstances (e.g., emergency, natural disaster, trauma, personal or family crisis) that directly compromised a student's ability to learn.
The board delegates to the superintendent or his/her designee discretion to grant a waiver of a maximum of two elective credits required for graduation. A student's parent/guardian or an adult student must file Bellingham Public School's Application for Waiver of High School Graduation Credits (Form 2418F) with the superintendent's office no later than thirty days prior to the student's scheduled graduation date. In order to graduate, students granted a waiver must earn thirty required subject credits (four English, three math, three science, three social studies, two world history, two art, three health and Fitness, one and a half career and technical education) which may be by satisfactory demonstration of competence as provided by WAC 180-51-050.
|Cross References:
Cross References: 2410 – High School Graduation Requirements
|Legal References:
|Management Resources:


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