text: Here's a question: has anybody tried those Quantum Dot antibody or streptavidin conjugates? Supposedly this stuff DOESN'T fade, ever. I'm looking into it, but have not tried them yet. www.qdots.com, in case anybody is interested. Kathleen Roberts Principal Lab Technician Rutgers University Gayle Callis wrote: > Rena, > > We do a great deal of immunofluorescent (IFA) and GFP work for both > fluorescent and confocal laser scanning microscopes. > > At 05:15 AM 8/12/2004, you wrote: > >> I have several questions related to storage of slides stained for >> immunofluorescence. I do know they should be viewed as soon after >> staining as possible, but it is not always possible for our >> pathologist to do so. >> How long can they be stored without loss of reactivity? > > > **Preferably, look at them the same day and depends on what > fluorophore you are using. Some fluorophores will fade faster than > others, FITC photobleaches faster than Alexa 488. Rhodamines come in > several derivatives, rhodamine (TRITC) photobleaches faster than > Rhodamine RX or RRX from Jackson Immunoresearch. We now use Alexa > fluorophores as much as possible, due to less photobleaching plus > extremely bright - or use a Strepavidin Alexa conjugate with > biotinylated primary or secondary. > > ****Hopefully IFA staining is done under cover of a dark towel or > foiled staining chamber and NEVER on an autostainer where you cannot > protect fluorophore from light. Don't start photobleaching before > viewing or storage! > >> Do you store them in the fridge? > > > **You can. We have stored them in slide cardboard folder, in dark > and on occasion in a freezer. The latter is not popular since slides > must come room temperature for viewing. We often look at them the > next day, try not to prolong this time too much - expensive in terms > of time and material, plus painful to lose fluorescent signal. > > The biggest help is use antifade mounting media that reduces loss of > fluorescence aka photobleaching. Molecular Probes, Prolong Gold > ready to use is ideal for this purpose. It must be stored in -20C > freezer with dessicant, thaw (in my pocket!), use, then return to > freezer after use, but the benefits outweigh handling and fluorescence > loss. You must seal edges of coverslip with diluted permanent mounting > media (dilute with toluene or xylene to consistency of thin top coat > nail polish, but do NOT use nail polish itself.) This media is > pricey, but worth its weight in "gold". It sets up hard and since it > is protective, sections can be viewed again. Sections still need > protection from all light - never on an open slide tray. Go to > www.probes.com/pl/prolong and look at the chart on fluorophores with > %loss using other media and Prolong Gold. It also works with GFP nicely. > > **Vector has VectaShield Hard Set that is supposed to work also. It > is nice to have an aqueous mounting media for fluorescent work that > sets up hard!! > >> After storage for several days, do you see more background, less >> background? > > > No more background than before, there are different reasons for > background, do you mean autofluorescence? or background from > staining? If the fluorophore photobleaches (fades) yes the > autoflourescence background would be more apparent. Preventing > background due to crossreaction of immunoglobulins to tissues, or > aldehyde fixation is possible. Proper blocking, dilutions, etc are > part of this. > >> After storage does your slide appear dirty when viewing >> microscopically? > > **No, if you have a dirty looking slide (I do not know what you mean > exactly, particles that sit on a section and fluoresce? If the latter > happens (glowing garbage!) then spin a diluted fluorophore conjugated > antibody just before application and take aliquot off top and apply > that to section. Antibodies conjugated to fluorophores, even sitting > around in refrigerator OR when thawed, have protein aggregates (sp?) > with fluorohore attached, and this bright junk will sit around on top > of section - microcentrifuging briefly eliminates this problem. > > If you do what we do, mount a coverslip on a slide taken from buffer, > the buffer salts sometimes leave a tidge cloudy film on back of > slide. After coverslipping, wipe back with kimwipe damp with water - > takes off buffer salts, all is clear. > > Last suggestion, go to Molecular Probes and get their Handbook - a > wonderful, educational, free book on handling and storage of > fluorophores, and multitidbits on fluorescence related products. I > believe you can download it too. > > Oh my, what a long discussion, been there - done it too many times > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 (lab with voice mail) > 406 994-4303 (FAX) > > > > _______________________________________________ > Histonet mailing list > Histonet <@t> lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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Hostiles R
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DetailsAction/Adventure , Drama
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In 1892, a legendary Army captain reluctantly agrees to escort a Cheyenne chief and his family through dangerous territory.
DetailsAction/Adventure , Drama
2 hr. 15 min.
Opened January 19th, 2018
Rated R for strong violence, and languageCastChristian Bale, Rosamund Pike, Wes Studi, Ben Foster, Stephen LangDirectorScott CooperWriterScott Cooper

subset: commoncrawl


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I talked to another of my favorite people from the harbor this week. Joyce Ibbetson, who has been the boating program director for UC Irvine for the past 34 years, took some time to answer a few of my questions.
I first met Joyce when I was a student at Orange Coast College and participated on the sailing team in 1982. At that time, she was involved in coaching the UCI sailing team, and both schools sailed out of the OCC rowing and sailing facility here in town.
Joyce grew up in Los Angeles and started sailing at the California Yacht Club's junior program in Marina del Rey. She attended UCLA and also was on the sailing team. After completing graduate school at USC, she returned to UCLA to coach sailing. She then was recruited by UCI to head the boating program in 1979 and has been there ever since. Over the past 34 years she has been in charge of the sailing, rowing and kayaking programs for UCI as well as the city of Newport Beach's sailing programs.
When I asked what her fondest memory as the boating director is, she replied, "It has to have been giving students access to the harbor and watching boating become a big part in their lives. We also founded the Anteater Regatta, which is a high school sailing regatta, and we founded the sailing team coaches' conferences that US Sailing is now running." During her tenure, she has replaced the 30-foot Shields sailboats with new J/22's and has started kayaking and stand-up paddle board lessons.
I then asked her what has been her biggest challenge. "Working with the ongoing budget restrictions has been difficult and will be overcome with time," she said. "We are also facing the problem of finding a new location to run our sailing programs from. Our lease with Orange Coast College will expire in two years, and we have been working with the city to find a new home."
Some time within the next couple of months, Joyce will be retiring, so I asked what the future has in store for her. "I plan on heading to Idaho and Europe this fall and doing some bike riding," she said with excitement in her voice. "Then I am going to see what types of doors open up and spend time with my friends, family and spend more time sailing again."
When asked to list three bullet points for the new boating director to make him or her effective, she replied, "Balance the past with the new, understand the old and make your own mark going forward. Don't sweat the small stuff and make sure you make time to go out on the water and sail. It's always good to look from the outside in."
Joyce has been on the harbor for some time now, so I had to ask her to comment on the state of our harbor. "It has gotten more crowded, which is good and bad," she said. "It's going to take more coordination with the harbor users to make sure events are planned. We need to keep educating people on water safety to avoid accidents when people are not knowledgeable. It's a good and bad challenge."
After racing in last Tuesday night's Taco Tuesdays out of Bahia Corinthian Yacht Club, a large group of J/22 sailors came down to the club for the awards presentation. The interaction between the UCI sailing club members, other harbor sailors and club members is priceless. The amount of access to the harbor the UCI boating programs provides is unmeasurable and, from my point of view, another one of our harbor's most valuable tools. If anyone from the nautical museum reads my column, I hope you will consider adding Joyce Ibbetson to our harbor's Extraordinary People Gallery. The service she has provided the community has set the bar at a new height and should be reflected upon by others who love the harbor as much as she does. We will miss you, Joyce, although something tells me we will still be seeing you on the water and making our harbor a better place.
Sea ya.
LEN BOSE is an experienced boater, yacht broker and boating columnist.


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Monica Haverkamp is ready to heap praise on the upkeep and cleanliness of Clinton State Park. Until she's asked about the beach.
As she laid out a towel and groomed a spot for tanning at Clinton Lake, Haverkamp ticked off some of the beach's problems: a lack of sand, weeds overtaking the perimeter, and a nearby culvert cutting across the shore.
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On this weekday, she's the only one on the beach.
"It needs a lot of improvement," she said. "People aren't using it like they used to because it's not as nice."
Clinton Lake's beach is a sign of the times at state parks in Kansas, Missouri and across the country as states scramble to preserve outdoor recreation while funding basic services such as schools, police and social services.
The nation's 7,975 state parks sit in a precarious position with shortened seasons, new admissions fees and threatened closures brought on by budget turmoil in recent years.
They also face mushrooming backlogs of repairs ranging from $26 million in Kansas to $750 million in Illinois to more than $1 billion in California. Park supporters estimate Missouri's park repair needs at about $400 million.
"It has reached a point where Band-Aids and baling wire are just not quite enough," said Steve Nagle, Missouri Parks Association president.
In tight budget times, lawmakers regularly put off park upkeep as they scramble to find enough money for basic government services.
"It's really not a good picture right now," said Margaret Walls, senior fellow for Resources for the Future, an energy and environmental policy think tank.
States have been gradually getting out of the business of bankrolling parks since the 1990s, sometimes cutting costs or replacing general tax dollars with new fees or dedicated taxes that are less vulnerable to the whimsy of legislators.
"There has been this death by a thousand cuts," said Richard Dolesh, vice president for conservation and parks at the National Recreation and Park Association.
In 1990, general tax dollars covered about 60 percent of a state park's budget. By 2011, that had dropped to 34 percent.
The spending cuts and delayed repair work, suggest some organizations, have left the country's park infrastructure in barely passable condition.
Earlier this year, the American Society of Civil Engineers gave the country a C- for the condition of its park infrastructure. It said states and local governments can't keep up with recreational needs because of shrinking budgets.
The group pointed to federal data showing that the states had an $18.5 billion wish list for outdoor recreational facilities for which there is no money, including $523 million in Kansas and $2 billion in Missouri.
South Carolina lists $155 million in deferred park maintenance. Texas estimates its backlog at somewhere between $400 million and $700 million, needing roughly $64 million every two-year budget cycle to maintain its system of more than 90 parks. This year, the state received $11 million from the Texas Legislature for capital improvements.
New York officials last year identified more than $1 billion in needed park work, including some sites where conditions were so bad that areas had to be roped off to protect the public. In some cases, amenities were closed off completely to ensure safety.
Last year, New York agreed to spend $89 million in park improvements, money that was used to attract private and federal funds for a total infusion of $143 million. It was billed as the the single largest sum of money spent on the state park system.
Illinois, meanwhile, this year started charging an extra $2 for license plate renewals to help battle a $750 million list of deferred maintenance at state parks.
Kansas reports a backlog of $26 million in needed maintenance at its 26 parks, many designed and built roughly 40 years ago.
The park system needs electrical upgrades to better serve snazzy new campers of the 21st century, replacement of aging open-air showers and rebuilding the beach at Clinton Lake.
A lot of work — replacing sewer lagoon liners for example — is largely invisible to campers. But it could ultimately prompt the closing of big swaths of a park or inconvenience campers if equipment breaks down.
"We've got some facilities that really need to be updated," said Kansas Wildlife and Parks Secretary Robin Jennison.
But the state budget for the fiscal year starting today only appropriates $10.6 million for parks, of which $9 million comes from user fees and lottery proceeds. The state plans to use $875,000 for capital projects. The parks budget in 2007 was about $10.1 million, but it received about half its money at that time from general tax dollars.
No general state tax dollars are going toward Kansas parks this year, compared with $5.1 million in 2007.
Jennison is trying to wean the agency off of legislative appropriations and make it self-sufficient without competing against other state needs.
"When you rely on general tax support you do not have the stability of income to make those kind of long-range plans," Jennison said.
For example, the agency arranged a deal to pay off cabins that were financed by the nonprofit group Kansas Wildscape Foundation, which raises money to help fund outdoors projects in Kansas.
In paying off the loan, the state gained access to an estimated $800,000 to $1 million in cabin fees that would otherwise have been committed to paying off the cabins.
The state also moved forward with a plan to sell annual state park passes at a $9.50 discount to drivers registering their cars.
Modeled after a similar plan in Michigan, the state hopes it can raise nearly $2 million more for parks by tapping into a broad base of potential park users who might be inclined to buy a discounted park pass when registering their car.
Jennison concedes the deferred maintenance list probably won't be wiped clean during his tenure at the agency. But he thinks the public will be satisfied with the condition of state parks.
"The average person going to the parks — I don't think they're going to see it," he said. "I think they're going to see some parks that are in pretty good shape."
But park visitors are pretty observant. They like the parks but see signs of wear and tear like wood benches in the showers that are decaying.
"They do an outstanding job with the facilities that they have to work with, but clearly the facilities are starting to show their age pretty seriously," said Clinton Lake camper Richard Lee of Topeka.
In Missouri, Gov. Jay Nixon this year persuaded lawmakers to put $20 million more into parks.
"Investing in Missouri's state parks today will help preserve our outdoor heritage," Nixon told legislators earlier this year.
But even in a state that receives sales tax money dedicated for parks, there's worry that Missouri is falling behind on its maintenance.
The state provided a list showing about $28 million in deferred building maintenance. Officials acknowledged it did not include other types of park infrastructure such as roads and sewers. The Missouri Parks Association said it has figures from the state showing the backlog at about $400 million.
Nagle attributed the money shortage to the state pulling its general tax funding after the introduction of a sales tax for parks in the mid-1980s.
Park supporters acknowledge that Missouri campers may not notice the aging infrastructure. But letting it go unattended risks that sections of a park could close if something malfunctions. They want the state to consider issuing bonds for upgrades.
"At least they can get started," said Susan Flader, past president of the Missouri Parks Association. "The $20 million is going to be just a small amount of what remains there to be done."


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With the most common form of cancer -- skin cancer -- on the rise, it's hard not to fret about soaking up too much sun this season. But instead of stressing your way through summer, take action to build up your skin-cancer defense, suggests dermatology expert Lily Talakoub, M.D., at the University of California, San Francisco.
For starters, take to the shade as often as possible from 10 a.m. to 4 p.m., when the sun's rays beat down the hardest. Whether it's sunny or cloudy, when you step outside, wear sunblock that offers an SPF of 15 (or higher) and contains zinc oxide or titanium dioxide.
"These ingredients physically block both UVA and UVB rays from penetrating the skin. They provide better protection than chemicals that work by reflecting the sun's rays," says Talakoub.
(Note that for adequate vitamin D levels, many experts recommend letting the rays shine on sunscreen-free arms and legs for 10 to 15 minutes two to three times a week.)
Remember the Basics Apply lotion 15 minutes before heading outside, and reapply every two hours. To keep your head sun-safe, wear a hat with a 4-inch brim (perfect for giving extra coverage to easily burned ears, face, and neck).
Be Observant
Check your skin once a month for changes or new growths to help guard against skin cancer.
Sip and Sprinkle
Recent research reveals that consuming green tea and curcumin (an anti-inflammatory compound found in the curry spice turmeric) may help combat skin cancer. Packed with potent antioxidants, green tea may also reduce DNA damage to skin cells when applied topically, according to animal studies.
In addition to choosing green-tea-infused sunblocks and lotions, drinking 3 cups of green tea daily and regularly including turmeric in your cooking may provide protection, says integrative physician David Rakel of the University of Wisconsin's School of Medicine and Public Health.
Keep Your Clothes On
Sunblock is a must, but it can't beat sun-protective clothing when it comes to reducing cancer risk, a 2007 study from the Lancet shows. Tightly woven garments in dark or bright colors (rather than white, pale colors, or pastels) are your best bet, says Talakoub.
For even more protection, look for UV-absorbing clothes labeled with the Skin Cancer Foundation's seal of recommendation.
Live for Lip Balm
Protecting your lips is key to preventing skin cancer, yet research shows that fewer than one in four Americans wear sun-protective lip balm. Talakoub recommends using lip balm with an SPF of 15 (or higher) and applying it 15 minutes before going outside.
"It's especially important to reapply lip sunscreens throughout the day, since they're easily wiped off with drinking and eating," she says. If you wear shiny gloss or lipstick, layer it over the protective balm. Some dermatologists suspect that glosses may increase the penetration of harmful UV rays.
Cover Your Bases
Women were more likely to develop melanoma (the most deadly form of skin cancer) on their chests in 2004 than they were in the 1970s, according to a study published in the Journal of the American Academy of Dermatology last year. So when you're showing a little skin, take care to apply (and reapply) plenty of sunblock to any exposed areas.


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Simeone: Nothing is impossible for Atletico
Diego Simeone has not given up hope of Atletico Madrid making the Copa del Rey final despite being 3-0 down after the first leg.
The cup holders were well beaten in an ill-tempered match against city rivals Real Madrid last week, and will need to record a big win if they are keep hopes of retaining the trophy alive.
Atletico have not lost at home in all competitions this season and Simeone still has hope that they can pull off a sensational second-leg victory.
"Qualification will be difficult but nothing is impossible in life," the 43-year-old said on Monday.
"We have to focus on improving.
"The team fight, play, work and do not look for excuses.
"I always said I want an Atletico team that made it uncomfortable (for Real) and we are getting there."
Atletico have a dreadful home record against Real, who have won their last seven games at the Vicente Calderon.


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Investment | Brussels, 21 June 2012
EU takes key step to provide legal certainty for foreign investors
The European Commission today took an important step to protect foreign investors at the EU level, thereby ensuring that Europe continues to be a safe and attractive place for investment by foreign companies. Today's proposal establishes a legal and financial framework for "investor to state dispute settlement" as part of a broad investment policy which has become an exclusive EU competence under the Lisbon Treaty. It is a further step in the creation of a comprehensive EU investment policy which will allow the EU to negotiate investment protection agreements at the European level. Today's decision will ensure the EU has the right system in place so that international investment rules can be managed effectively in cases disputes arise between foreign investors and the EU and its Member States.
A key element of investment protection is the possibility of dispute settlement between an investor and a state, a provision currently included in the more than 1,000 bilateral investment protection agreements concluded between EU Member States and countries outside the EU. Once investment agreements will be concluded at the EU level, foreign businesses investing in the EU may, for the first time, bring claims against the EU alleging that investment protection obligations have been breached. Today's proposal clarifies who would bear the financial responsibility when compensation has to be paid – Member States or the EU. It ensures that foreign investors in the EU are not affected by this allocation of responsibility.
"Investment is a driver of growth so it's essential that we have the right rules in place. Just as we expect EU investors to be duly compensated when the rules are broken, we have to make sure we have a system at the EU-level to follow suit. Today's proposal makes it clear who pays. This is one step in an overall strategy to ensure Europe remains an attractive investment environment", said Karel De Gucht, EU Trade Commissioner.
The EU is the world's leading host of foreign direct investment, attracting investments worth €225 billion from the rest of the world in 2011 alone. The proposed regulation will provide legal certainty and predictability for foreign investors, which will help keeping the EU open for investment and create jobs.
The Regulation proposes a mechanism whereby financial responsibility for the costs of investor-to-state dispute settlement and the right to defend such a case are allocated between the EU and the Member States on the basis of whose actions caused the investor to bring a claim:
- Where the measure which is alleged to be in breach of the agreement is a Member State measure, which was not required by EU law, the Member State would bear the financial responsibility flowing from the dispute and may in principle also act to defend the claim.
- Conversely, if the measure at issue is an EU measure or a Member State measure mandated by EU law, the EU would bear the financial responsibility and could act as defendant.
The regulation permits the Member States to defend their own measures where they would ultimately have to bear the costs, unless it is in the interest of the Union to act as defendant, on the basis of the conditions set down in the proposal.
In all cases, there should be very close cooperation between the EU and Member States in order to ensure the best possible defence of any claims alleging a breach of the investment protection agreements negotiated by the EU.
The Treaty of Lisbon included investment as part of the EU Common Commercial Policy, an exclusive competence of the EU. As a consequence, the European Commission can now negotiate on behalf of the European Union on both the liberalisation and protection of investments.
Today's proposal on investor to state dispute settlement is the third key part of this policy, which is based on:
- Negotiating new rules on investment with key trading partners
The EU is gradually negotiating on investment with key trading partners. Provisions on investment liberalisation and protection will be included in comprehensive Free Trade Agreements, or in self standing investment agreements.
The European Commission is currently negotiating on investment, including investment protection, as part of the Free Trade Agreement talks with Canada, India and Singapore, while the Council has recently adopted the negotiating directives also for four Euromed countries (Tunisia, Morocco, Jordan, and Egypt). The Commission is also preparing for an EU-China investment agreement.
When concluded, the EU level agreements including investment protection will replace the Member States' Bilateral investment Treaties with the same third countries.
- Ensuring the smooth continuity of existing bilateral investment treaties
More than 1000 bilateral Investment Treaties existing worldwide are in place between EU Member States and third countries.
To ensure legal certainty on these existing Bilateral Investment Treaties of the EU Member States the European Commission has presented a proposal for a Regulation of the Council and the European Parliament in July 2010 (IP/10/907). The Council and the Parliament have recently found a political agreement on the Commission's proposal, and the regulation is expected to be adopted and enter in force before the end of the year.
What happens next?
The proposal will now be discussed by the Council of Ministers and by the European Parliament under the ordinary legislative procedure.
The regulation should be in place before the new Free trade Agreements that contain new EU-level investment protection provisions comes into force.


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jgsketch necesitaba un nuevo diseño de página web y lanzó un concurso de diseño en 99designs.
Un ganador ha sido elegido entre 69 diseños de 18 diseñadores freelance.
DebtConsolidationConnection.com is a non-profit credit counseling agency with a focus on education and providing resources to the public.
Our audience is anyone who is in debt or are seeking information about finances and money management.
This contest is for graphic work only (no coding). We are looking for a finishing touch to our website, http://www.debtconsolidationconnection.com. Our main focus is on the header with smaller focus on overall color scheme and side bar. We are not looking for a redesign as we like the current layout. We would like to have a more eye-catching header that has a clean look, new logo, call of action improvements and a better color scheme.
This site will be a lead generation site as well as a site for major press releases. Our recent press release has been picked up by over 200 TV stations nationwide and many daily and weekly papers as well as online. Point being a lot of press will be on the site. The site needs to press friendly & lead generation friendly simultaneously.
The current logo was pulled from another site we own and does not go with this site at all.
The call of action on the right should be redone with a little more emphasis. All though this site is more about the education and resources than it is about lead generation we still need to generate leads.
The color scheme should be similar. We like the dark background and blues of the site.
While the side bar is not the main focus of the project we would like to see a touch up to the form and menu section.
A PSD file has been uploaded of the current design. http://www.debtconsolidationconnect…insite.zip


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Relationship Banker CP - 1700 K Street
At Capital One, we are passionate about providing an exceptional customer experience while helping our personal and small business clients achieve their financial goals. As a Relationship Banker (RB), you will have the opportunity to use your love of working with people, needs-based solutions, and product knowledge to play a vital role in our customer's banking experience. You will partner with other branch associates to provide innovative solutions for our customers.
We are looking for smart, ambitious and talented professionals to develop relationships, consult with clients and analyze which products and services best meet their needs. Join us and collaborate with other creative and goal-oriented team members in an environment where you can achieve your full potential.
This position is the first, role for an in-branch banker. The RB is responsible for proactively developing, managing, retaining, deepening, and growing customer relationships of our new and existing Consumer and Small Business customers. The Relationship Banker provides exceptional service through demonstrated ability to identify customer needs and consult customers by conducting needs-based assessments and educating customers on our products and services.
An RB must maintain set service goals, demonstrate skills-based behaviors, and acquire knowledge of complete line of products and services offered at the bank while working towards Relationship Banker certification. An RB must consistently demonstrate Service Excellence behaviors in their interactions with customers and in customer survey feedback.
This role requires you to obtain Relationship Banker Certification.
Requires successful registration with, and issuance of a unique identification number from, the Nationwide Mortgage Licensing System (NMLS) in compliance with the Secure and Fair Enforcement for Mortgage Licensing Act of 2008 (S.A.F.E. Act).
General Responsibilities:
- Live the company values of Excellence and Do the Right Thing
- Act as a Customer Advocate
- Contribute to a customer-centric culture, continuous improvement, innovation and change
- Actively contributes to team's success
- Own personal development and achievement of required responsibilities and skills-based behaviors to obtain Relationship Banker Certification status; and recertify annually
- Consistently use needs-based assessment tools to analyze and determine customer needs
- Meet with prospects and customers as a result of needs-based conversations in-branch and from other lines of business
- Leverage tools to recommend appropriate solutions in person, on the telephone or via other channels
- Maintain a working knowledge of the complete line of products and services offered; takes responsibility to keep up to date and request assistance for further development needs
- Take an active role in educating customers on other banking channels as well as other products and services
- Fulfill any of our customer's servicing needs with the intent of enhancing customer retention and engagement
- Ensure all audit and security policies and procedures are followed in accordance with Bank policy and Federal regulations
- Able to travel between branches in assigned local area
Basic Qualifications:
- High School Diploma, GED, equivalent certification, or military experience
- At least 1 year of Customer Service experience or at least 1 year of Sales experience
Preferred Qualifications:
- 2+ years Sales experience
At this time, Capital One will not sponsor a new applicant for employment authorization for this position.
Requires successful registration with, and issuance of a unique identification number from, the Nationwide Mortgage Licensing System (NMLS) in compliance with the Secure and Fair Enforcement for Mortgage Licensing Act of 2008 (S.A.F.E. Act).


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So my Boyfriend is a HUGE meat-eater and I'm not.
so you have any good, quick, and easy recipes that involve some sort of meat but is also healthy? (like no fried chicken or stuff)
Thanks! :)
I don't know if this counts as a 'recipe' per se, but my boy seems to like it. I get some of those 'gourmet' style burgers pre made from the supermarket, cut them into quarters, give them a quick reshaping, cook, freeze and done! Straight from freezer to bento for a quick meaty thing.
that's a good tip!
I have a 'gourmet' supermarket near home so I'll try.
My bento blog: http://justbento.com/blog/1305
My art blog: http://jizaacaso.deviantart.com
I don't know if this counts as a 'recipe' per se, but my boy seems to like it. I get some of those 'gourmet' style burgers pre made from the supermarket, cut them into quarters, give them a quick reshaping, cook, freeze and done! Straight from freezer to bento for a quick meaty thing.
At that point, you may as well make your own, either from good quality mince or by mincing it yourself.
Chicken sceweres, cold sausages, and just plain old ham sandwiches are all most welcome in Creatures lunchbox. Mind you, he's not all that adventurous.
I usually make a stash of this dish. It is very tasty, it also turns out very sauce-y but you can put it in a silicon mold so it won't spoil the rest of the food in your bento. I never used it in a bento, though, but I guess it would be ok. Or you just could store it in the fridge and take only the meat forgetting about the sauce.
The quantity of veggies in this recipe goes absolutely to taste. i find it the better with a bigger amount of veggies but this goes to taste)
- 1 or 2 onion, sliced
- olive oil
- carrots & other veggies like green peppers and so (absolutely optional), cut in slices
- like 1/2 kg of beef meat cut in chunks (they should be like 3 cm long maximum)
- a pinch of salt
1.- just heat up to medium heat the olive oil in a pan with the salt. Put enough oil to cover well the bottom of the pan.
2.- when hot, add the onion and cook it until half cooked.
3.- Add the rest of the veggies and cook until done.
4.- Remove the veggies from the pan. Add another olive oil spoon or 2. Add the meat and cook until brown in every side. Be sure that it's cooked in the inside. (I particularly like meat well cooked).
5.- When the meat is done, add the veggies and cook a little more time. You can add some more oil if you see that the whole thing is getting a little dry.
I find this good for bento because the meat chunks will be good at room temperature :)
If you make this the night before, just microwave it a bit or re-heat it in a pan with a little oil before you put it in the bento :)
I hope this helps!
This is really helpful! this also might be a way that I can sneak vegetables into his diet! n_n Thank you!
I know! My husband is also a carnivorous and I'm fighting for the veggies! lol
One of the easiest ones is a family recipe of mine, I have never had it but everyone I have every made it for love it. Sorry I can not be very precise with the recipe, I have never measured.
-chicken (I usually use breasts or thighs, but any cut or size piece will work)
-soy sauce (enough to cover the chicken)
-orange juice (a splash or two)
-finely chopped garlic (a clove or two for a lb. or two of chicken, more for more chicken)
Toss everything in a plastic bag, mix it around. Let it marinade over night to a full day in the fridge, turning or mixing every so often. Bake or BBQ as you would any other type of chicken.
Duly noted! Thanks! and don't worry about exact measurements! I hardly ever measure anything anymore! :D
Soy sauce and orange juice??? I must try this :D
You might also like to try soy sauce and beer.
I originally learnt about it after reading a book where the protagonist makes himself a stir fry with strips of beef and green pepper using soy sauce and beer (I think the book was The Wind Up Bird Chronicle by Haruki Murakami*). I tried a non-meat version myself with a little oil and added toasted sesame and it came out really well. Now, whenever I have some leftover beer (which isn't that often) I combine it with soy sauce and add it to a hot pan of vegetables. Ginger goes well with this too. I often prefer it to soy sauce and mirin (teriaki) as a quick and easy cooking sauce. I'm sure soy sauce and beer is very good with beef and other meats.
EDIT: * I just found the quote where it was mentioned online
"I would be stir frying thin slices of beef, onions, green peppers, and bean sprouts with a
little salt, pepper, soy sauce, and a splash of beer-a recipe from my single days. The rice was
done, the miso soup was warm, and the vegetables were all sliced and arranged in separate
piles in a large dish, ready for the wok"
(I think the book was The Wind Up Bird Chronicle by Haruki Murakami
that's one of my fav books! :D :D :D
I'll look up for the recipe :D
This sounds scrumptious :P
Adding it to my list lol
Thank you
I'm getting my Bento on:P
I wanted to chime in with a suggestion for Maki's basic meat & tofu mixture for meatballs. As a Dane who loves her frikadeller (our national meatball), I was a little uncertain about using tofu mixed in with the meat. I love tofu as the star ingredient in hundreds of dishes, but in meatballs? Thing is, they really did made a lovely addition to meatballs, adding a tender, toothsome lightness, with the added bonus of some vegetable protein. So I'm a big fan now, and maybe your carnivorous boyfriend will be too :-)
You can make them into meatballs, mini-burgers, stuffed tofu or mushrooms, mini-meat-pies --the possibilities are endless! Eaten plain, they're great, and it takes 3 minutes in the morning to whip up a piquant sauce which makes them even tastier. They freeze really well --If I'm in a hurry I defrost them for no more than a minute or two in the microwave, and when they're still cold but unfrozen, I tip them into a little frying pan with a quick sauce of, say, ketchup, soy sauce, Worcestershire sauce, and a splash of any wine I have sitting around, and voila! Yummy meat for my bento. I learned this all over at Just Bento: http://justbento.com/handbook/johbisai/meat-tofu-mixture-mini-burgers-me...
For me, it'd be chicken or beef marinated in soy sauce, sesame oil, ginger, garlic and lot of black pepper. Adding some honey to the mixture works very well too! You can also try with hoisin sauce, but remember to use less soy sauce. AFter that, just leave them in the fridge for about 8 hours to let the marinade seep through (or freeze them when you're not using, but defrost when you're about to cook). I will then add some sliced onions and red peppers when I cook them.
Another option would be miso and kochujang (korean hot-spicy paste, I think Maki-san uses it too) which happens to work well with veggies too. You can add soy sauce if you think it needs more taste or chilli flakes to make it more spicy. And then, the same set of onions and red peppers go into the pot when cooking.
If you want them sweet and sour, you could try adding chicken/beef bullion, lime juice, sweet chilli sauce, salt and white pepper. Onions and red peppers then go into this one too ^^; But this time, I shall call for fresh ripe pineapple chunks and zucchini. I dare not try adding soy sauce and tomato sauce because I'm afraid they might "spoil" the flavors. Garnish with some fresh parsley before serving =p
Sorry for not being able to give any measurements because I simply dump and taste ^^;
The other night I actually look some ranch dip mix (the powdered kind) and mixed it with some olive oil and then used it as a marinade for some chicken breasts. After they had a chance to sit in the fridge while I worked, I cooked them up in a grill pan. I was shocked by how much my boyfriend liked it. Hooray for easy weeknight meals!
I have a blog about life and bento making in Boston: www.smalerie.com
My mom does that, and melts a bit of cheddar cheese on top just before it is done.
She does the same thing with chicken that she cooks in cream of mushroom soup.... I don't eat meat so I don't know how they taste, but I have never heard any complaint [besides myself, because I will not eat noodles or vegetables cooked with the meat].
The other night I actually look some ranch dip mix (the powdered kind) and mixed it with some olive oil and then used it as a marinade for some chicken breasts. After they had a chance to sit in the fridge while I worked, I cooked them up in a grill pan. I was shocked by how much my boyfriend liked it. Hooray for easy weeknight meals!
Another version of this is instead of the Ranch, use the French onion soup packets with Olive oil - then either put it in the oven or pan fry.
This same thing also works BEAUTIFULLY with chopped up potatoes - the onions get crispy and it's salty and savory. I was making it waayyyyy too much there for awhile. And for the guy who is only meat + potatoes - he loved these too!
Wow, I never thought of that! I've used french onion soup packets for meat loaf before, butI should really try it on potatoes! Thanks!
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Evansville Teachers Federal Credit Union and Credit Union Student Choice want to hear from you and your experience with your private student education financing.
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In December 2016, the Federal Reserve raised its benchmark interest rate, and there is reason to believe the rate will increase again in the near future. So how does this affect you?
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CEC member P.Vivekanandan reflects on his experience at CBD COP 10 with a delegation of the LIFE Network, Local Livestock for Empowerment.
SEVA, Sustainable Agriculture and Environmental Voluntary Action, our NGO in Tamil Nadu, India, was one of the participants of the eight-member LIFE Network team for a visit to COP 10 at Nagoya from 18 to 24 October 2010. LIFE Network stands for 'Local Livestock for Empowerment' and promotes livestock keepers' rights. It was an opportunity created by LPP.
There was a huge gathering of participants from various parts of the globe symbolically representing their culture, spirit and yearning for an unknown mission. When we see the logo and slogan of CBD COP 10 my doubt in the culmination of cultures become clear. The logo illustrates the harmony of humanity and diverge living beings with a human of parent and child at the centre of a circle of origami plants and animals. The parent and child expresses the desire to pass on abundant biodiversity for future generations. By arranging diverse animals and plants made by origami this logo illustrates the diversity of life on Earth. The slogan for the meeting was "Life in harmony into the future" (Origami symbolizes the culture of Japan and the wisdom of human beings).
We arrived the COP 10 the second day after its inaugural meeting on 18 October 2010. In spite of missing the inaugural we grasped the objectives of CBD: to review its progress in implementation, consider amendments and to consider the adoption of protocols on Access and Benefit Sharing (ABS).
There were many parallel meetings and working groups on various field of biodiversity : the Rio convention's ecosystems and climate change pavilion; the fair on experiences and best practices in communication, education and public awareness; and more than 300 side events slated during 18 to 29 October, plus daily events on Indigenous knowledge and local communities .
We attended an informal meeting on bio-cultural protocol of pastoral communities and we were able to attend few side events and my observations are given as below:
Biodiversity is Towards Sustainable Agriculture
FAO in collaboration with United Nations organized a side event on "Biodiversity + Agriculture" on 20 October. Among the best practices presented were findings that monocropping with tea bushes leads to soil erosion in hilly terrain while intercrop with agro forestry conserves soil from erosion; similarly in banana there will not be risk of "moko" disease incidence if it is planted along with Heliconia sp.
We raised a question that agriculture or forestry without livestock is not sustainable but nowadays pastoralists are being labeled as enemies of forests. To my question on this FAO ( Parviz Koohafhan, Director, Land Division of FAO) has agreed that a case study can be initiated on this aspect, which gave satisfaction to me.
Biocultural Protocols (BCP) and ABS
We had an opportunity to present our experiences of Biocultural Protocols on 23 October during a informal meeting with LIFE Network members in a space provided by United Nations University Stall (through Sunitha Subramanian, Fellow of United Nations University) . Presentations were made on Raikas of Rajasthan, Lingayats of Bargur Hills, Tamil Nadu, Banni pastoralists of Kachchi, and Samburu of Kenya. These traditional communities are rich in tradition, customary practices, conserving local breeds but denied with traditional grazing rights in commons or forests or getting any recognition for the animal genetic resources they are keeping. Nowadays the population of local breeds is reducing and there were no incentives for them. The meeting was facilitated by Hary and Holy of Natural Justice, South Africa. There was a debate on BCP, and it is for communities or for a specific area where different communities use a common land/territory.
BCP is an effort for dialogue in order to reinforce their rich traditional knowledge , practices and genetic resources they conserve. In this process, the community became aware of national or international laws. Based on this they develop their own charter.
Article 8 j of CBD speaks of traditional knowledge, innovations & practices while Article 9 on knowledge associated with genetic resources and propose to develop their own community protocols.
ABS protocol may not cover interests of communities except very commercially potential practices. Under ABS, non - commercial traditional practices are squeezed out. Biodiversity is not for trade. Therefore customary rights are to be secured as per Article 10 of the CBD.
Pachamama (Indigenous Knowledge Newsletter of CBD):
It is a quarterly newsletter of CBD Secretariat which speaks about article 8 ( j ) , rights of indigenous peoples, co–management of resources while respecting indigenous knowledge and innovations. Compared with our Honey Bee Network http://www.sristi.org/hbnew/, we restrict only knowledge or practices but tells the ways of non commercial rights, spiritual values of communities.
In one of the side event there was a debate on definition of terms such as traditional, local, indigenous communities. In Latin America terms such "Indigenous people" or territories apply where as in Africa, Asia it is "local or indigenous communities".
When we focus our programme only to indigenous communities then the other local communities are deprived of incentives. For example, in South Africa, are whites settled 1652 indigenous or only Bushman of Africa?
In all the presentations land, knowledge and spirituality cannot be separated.
In one meeting, innovation was a product of indigenous knowledge + scientific aspect of practice. The best way to operationalise an incentivized approach is devolve rights at lowest level or in community access to resources.
Land day 3
Mr Ahmed Djoglaf , Executive Secretary of the CBD, stressed the need for a synergy between various actors/conventions, and ultimately touched upon a farmer or community in a Land day 3 event organized by UNCCD. It does not matter much if 'A' programme intervenes on a particular theme and other programme on a different theme. But at farmers/community level there will not be any separation.
We had on occasion to tell that soil enhancement without livestock is meaningless and there is no contradiction of land degradation due to over grazing. Now the UNCCD is able to grasp the intrinsic role of livestock in dry land in terms breed or improving the environment or deriving income from sustainable livestock keeping system and reflected in their summary of statements made by facilitators.
In a Landscape conservation event it was said that, "Protected areas will alone not help for climatic change". In the reality of biodiversity management, agriculture and forests departments do not speak together. We want a holistic approach as stressed .
If 'Satoyama' is a successful model of Landscape Conservation that Japan wants to tell others about, then our indigenous knowledge-rich farming system and natural resources are indispensable for maintaining agro-biodiversity and adaptation mechanism for climate change. To my understanding, COP10 is nothing but events of communication on co-management system of commons while respecting traditional knowledge or innovations for finalizing a protocol on ABS. Let us join hands at COP 11 at India in October 2012 and disseminate the Honey Bee Network / LIFE Network model we initiated in our country using indigenous knowledge and innovations.
P.Vivekanandan, SEVA, Tamil Nadu, INDIA, at firstname.lastname@example.com


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Nation's Best Collegiate Athletes Meet at University of Arkansas
NEW HAVEN, Conn. - This week, the track season will finally come to a close for the nation's top athletes. The University of Arkansas will host the NCAA Championships on the John McDonnell Field in Fayetteville, Ark. starting on Wednesday. Yale sophomore Kate Grace (Los Angeles, Calif.) will be competing against the country's best 800-meter athletes in this prestigious meet.
Grace is one of a field of 27 athletes from all over the country competing in the 800-meter run, all of who had to qualify based on their performances at the NCAA Regional Championships. The Regionals were held over a week ago in four different regions of the country: East Region Championships were at North Carolina A&T State University, Mid East Region at the University of Louisville, West at the University of Oregon and Midwest at the University of Oklahoma. The top five finishers in the finals at each regional meet automatically qualified for the NCAA Championships, while seven other athletes in each event were given at-large bids based on their season bests.
Grace finished third at the East Regionals in Greensboro, N.C. with a time of 2:07.43, after an amazing performance in the qualifying heat the day before, when she ran 2:06.69, her fastest time of the season.
Grace is currently ranked 21st in the field of 27 athletes with her 2:06.69 time. She is the only Ivy League competitor in her event. The top-seeded runner, junior Phoebe Wright of Tennessee, has a personal best of 2:02.15 (last year Wright finished sixth in the finals). Last year's champion, senior Geena Gall of Michigan, is ranked second with a time of 2:02.29.
"These girls are the best in the event," Grace says. "I know them all by name - I've heard them and I've seen them at various meets over the course of the season. I'm not really close with any of them, but after awhile you get to know them by sight and by reputation. I'm really excited for the competition. They'll be good races."
The NCAA record in the 800-meter run is 1:59.11, set in 1990 by three-time Olympian Suzy Favor. The only Ivy League competitor who has ever won a NCAA title in the event is Harvard's Meredith Rainey. As a team Yale has never placed above 20th, though the Bulldogs send competitors nearly every year, and they have routinely scored.
Grace is no stranger to big championship meets, having competed at the outdoor NCAAs last year, and the indoor NCAA Championships in March. Last year Grace was one of only three freshman in her event at the NCAAs. She received an at-large bid, and ended up finishing 24th with a time of 2:09.47. On her way to the indoor NCAAs, Grace repeatedly broke the school record in the 800-meter run, with her fastest time at the Championships: 2:04.72. She earned All-American honors for her eighth-place finish in the finals.
Grace compares the experiences of the NCAAs: "The outdoor competition is bigger: literally bigger and better competition. Because there are more competitors, there are three days of races, which is different, and much bigger crowds, which is fun. It's incredible - a really special kind of event. You have to stop and take it all in. We have practice with crowds that large at Penn Relays, but normally track is not the biggest spectator sport, so it's always exciting to have that many people. Last year in Iowa - they have a big track culture there - there was a huge banner welcoming all of the NCAA athletes. Every hotel is booked with athletes - we completely take over the town. It's just such a special event."
In this outdoor season, Grace won the Heptagonal Championships (2:09.01) for All-Ivy honors and the ECAC Championships (2:08.44 finals, 2:06.75 preliminary) for All-East honors.
Grace has stayed at Yale to train all through the Championship season, after most Yalies have returned home after their exams.
"I've been weight training with Coach every other day, and then on off-days I run on my own," Grace explained. "I've gotten some of the guys on the men's team to train with me, which makes a big difference - it's so nice to have a training partner. I can't train with Jeff Lachman [Yale senior competing in the discus throw at NCAAs], clearly, because he's a thrower, but there are other guys who are in New Haven for summer jobs who I can run with."
Because the meet is so large, there are three rounds of competition at the NCAA Championships. Grace will run one of the four preliminary heats on Wednesday the 10th at 5:30 p.m. CT. If she is one of the top three finishers in her heat, or if she is one of the next four runners with the fastest times, then she will make it to Thursday's semi-finals (7:15 p.m. CT). If she can finish in the top three, or have one of the next two fastest times, Grace will qualify for the finals - the nation's eight fastest 800-meter runners - scheduled for Saturday the 13th at 12:19 p.m. CT.
The meet will be broadcast live at CBSSports.com, and live results will be recorded at FlashResults.com. Results will also be posted at the end of the day at YaleBulldogs.com. More information about the NCAA Championships can be found at NCAA.com and at ArkansasRazorbacks.com.
Report by Kyle Eichner '12, Yale Sports Publicity


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WALNUT, Calif. – Lipscomb's track and field squads competed at the Mt. Sac Relays for their first day of weekend competition.
The team struggled in their search to post better results than those seen the past two weeks.
"Today did not go as hoped for the Bisons at the Mt. Sac Relays," said Luke Syverson, assistant track and field coach.
Tucker Peabody was scheduled to compete in his best events, but couldn't extend his successes on Friday.
"Tucker struggled in both the 100 meters and the 200 meters today," Syverson said. "He looked uncharacteristically sluggish for some reason so we'll have to learn from today and move forward."
Peabody finished the 100 meters in 10.67 and the 200 meters in 21.21.
Andrew Bosket saw his own set of struggles in the 800 meters. He finished out the event with a time of 1:54.81.
"The race started out real fast and was pretty aggressive in terms of positioning with lots of elbows and pushing," Syverson said. "Andrew stayed out of it for the most part but left himself too much track to catch up."
Colin Dempster fought hard in the 400 meters but ultimately failed to record a top finish, crossing the line in 47.93.
"Colin had to race out of lane one and finished second in his heat but got out of the blocks slow," Syverson said. "I think overall, this was his second fastest time of the season but he was looking for more and could have gotten it."
Overall the group did not perform up to par, but will still use Friday's results as a learning experience.
"I told the guys afterwards that 'experience is what you get when you don't get what you wanted,' and we definitely got experience today," Syverson said.
The competition will continue Saturday where Gemikal Prude will be putting his best foot forward as he competes in the elite high jump.
"Saturday will be a big day for Gemikal as he will be jumping against some of the best high jumpers in the U.S. and the world," Syverson said. "It will, at the base level, be a good experience that will pay off in future competitions. We look forward to how he competes and responds to the opportunity."


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Berlin Skyline Building Set from LEGO®
Capture the architectural essence of Berlin with this magnificent set that brings together the iconic Reichstag, Victory Column, Deutsche Bahn Tower, Berlin TV Tower and the Brandenburg Gate, in an inspirational skyline setting. Each individual LEGO® structure is designed to provide a unique and rewarding building experience, with true-to-life color and relative scale depiction. This wonderful model also features 3 sections of the Berlin Wall with graffiti deco, adding a historical edge to this detailed recreation of one of the world's most compelling cities.The kit contains 289 pieces and a booklet with details on the buildings' designs and histories. Recommended for ages 12 and up, but great fun for adults too! This product has been retired.
Size: 9" x 9" x 1"
Code: 75871
Member price: $67.50
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Interactive Panoramic Viewer
FreePV is part of the PanoTools Software Universe and is an effort to make a universal viewer for the different VR file formats. Thus these projects intend to add new features like basic support to new files and features like hotspots that are essential for the user interactive experience. One of the main objectives is to add a basic support to SPi-V file formats and better one for QTVR files to Linux users, where neither shockwave player nor Quicktime is avalaible, so the mozilla-plugin and glut freepv implementations should be able to read basic files to the end of this project.
The first part of the project will consist in extending the libfreepv component, so it has a more flexible internal data structure to handle different panoramic formats, this internal data structure will help as an interface between the platform and the different file formants. With this data structure, you will need just to implement the file decoder module, which you desire. These modules should fill this internal data structure. At this moment PanoViewer (part of libfreepv) uses the QTVRDecoder data structure, but by the end of this project it should handle the QTVR file data through this more flexible data structure.
The second part will consist in adding a basic SPi-V XML file format support, this will consist in adding an XML parser and implementing the SPIVdecoder, which will fill the new data structure, for this purpose I will need to read in detail the SPi-V XML file format specification. To the end of this part, the viewer should be able to display basic SPi-V panoramas; at this point the decoder will handle data from the meta node.
The last part of the project will consist in enhance the user interactive experience adding the support to hotspots; this will enable the user to make a virtual tour, switching from one scenes to another by just clicking over the hotspots. This last feature should be supported for both QTVR and SPi-V file formats, in order to this I will need to check the Scene class, and design a component to handle the different scenes of a tour. Furthermore most of the hotspots in SPi-V are made with images that not necessarily are JPEG, so it will be needed to add support to files like png and gif.
The hotspots could be points, squares or polygons, for the design of the hotspot interface, it should contemplate these possible configurations, and I'll need to check the EventListener class and to implement a basic collision detection algorithm. The hotspots will support basic events, like roll over and click. Taking advantage of the new image readers formats and the SPi-V file format, it would be nice to be able to display flat images with the panoramic views.
QTVR hotspots [1] SPi-V Panoelement (hotspots)[2]
1. Make SPi-V file format avalaible for Linux/Unix users. 2. Add freepv support to Hotspots. 3. Add freepv support to load flat images.
1. Planification 2. Design 3. Implementation 4. Testing period
April 11: Begin a deeper familiarization with the project code, set up every thing (lib, compilers, etc…), so that I don't have problems to begin coding.
April 23: Read articles, literature, papers and specifications, solve any doubt left with the mentors and the community.
May 9: Read with more detail the files specification and finally discuss with my mentors and the project community some implementation details.
May 28: Begin coding the data structure that most be part of the libfreepv component.
Jun 4: Begin coding the file decoder class using the XML parser and planing the new image readers (PNG,GIF).
Jun 18: Begin with the Testing period.
June 29: To this date the file decoders classes must be stable, be crossplataform and they should be well documented. Begin with the image readers implementation and begin the planification of hotspots.
July 9: Students upload code to code.google.com/hosting; mentors begin mid-term evaluations. I will begin codifing hotspot.
August 13: To this date the hotspots should work, be crossplataform and well documented.
August 20: Students upload code to code.google.com/hosting; mentors begin final evaluations; students begin final program evaluations
August 31: Final evaluation deadline; Google begins issuing student and mentoring organization payments


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Items where Author / Artist / Editor is "Sandini, Silvia"
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De Bernardis, Flavia and Liu, Haiqun and O'Mahony, Rachel and La Valle, Roberto and Bartollino, Silvia and Sandini, Silvia and Grant, Steven and Brewis, Neil and Tomlinson, Ian and Basset, Rachel C. and Holton, John and Roitt, Ivan and Cassone, Antonio (2007) Human domain antibodies against virulence traits of Candida albicans inhibit fungus adherence to vaginal epithelium and protect against experimental vaginal candidiasis. Journal of Infectious Diseases, 195 (1). pp. 149-157. ISSN 1537-6613


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Invent a new cheese product for office snacking or evening cheese and crackers.
In Britain, people eat Cheddar. In every fridge in the UK you will find Cheddar. There are many ways of eating it, in a sandwich, over jacket potatoes, on hot pasta, as an on-the-go snack or enjoyed on its own.
Seriously Strong is one of the most popular brands in Britain. It's particularly known for its strong and characterful taste that makes it stand out from the other cheese brands.
Seriously Strong makes sliced cheddar, grated cheddar, cheese sauce and the unbeatable block Cheddar in fuller-flavoured strengths (Mature, Extra Mature and Vintage).
Invent new Seriously Strong cheese products so Brits have new and interesting ways to enjoy characterful cheese during office snacking & evening cheese and crackers (and wine!).
Presentations: design and text explanation (5 pages PDF)


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Welcome to our bag and backpack shop
With the new pro design® shop 2008 come and share our passion for one of the most effective and desirable promotional items: the bag! Bags and backpacks are unrivalled as products which can express the very soul of each promotional campaign while providing the target group with practical and enduring items that will be used repeatedly and convey the desired image over a long period.
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You will surely find some stimulating and interesting ideas while paging through the shop. Whatever your needs do not hesitate to contact us for advice and immediate reaction to all your questions.
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* Jose Reyes was scratched from today's game for additional bloodwork. Reyes has subsequently cleared to play and will take batting practice later today. The Mets said scratch had nothing to do with his hamstring injury.
The Mets are home to St. Louis tomorrow and Reyes is expected in the line-up.
* Closer Francisco Rodriguez is still bothered by a case of pink eye and has not been cleared. Considering relievers need far less time to get ready for the season there is no worry Rodriguez will be set back to where there is a concern.
* The Mets have set their rotation through Tuesday. Jon Niese will start tomorrow against the Cardinals, followed by Mike Pelfrey, Saturday against Washington at Viera; Oliver Perez, Sunday against Washington at Port St. Lucie; John Maine, Monday against Florida at Jupiter; and Johan Santana, Tuesday against Houston at Port St. Lucie.


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"What's the degree of bend in this paddle?"
As you may remember from the summer of 2010, I wrote a couple of articles entitled "My favorite questions as an outfitter." And while the above question may have been in contention for inclusion in that list, it might be a good inquiry. My first reaction was to think this guy was some kind of gear geek, and had been reading too many canoe forum paddle posts. But in reality it is a question that should be answered.
Let's start with the basic, bent-shaft canoe paddles vs. straight-shaft canoe paddles.
In the early 1980's canoe racers discovered that they could increase their stroke efficiency by bending the shaft of the paddle between 12 and 14 degrees. The bend changes the mechanics, and creates a stroke where the blade of the paddle stays virtually upright through the arc. The transfer of power from the paddle to the water is almost entirely horizontal.
Through the arc of a straight shaft paddle motion you have three transfers of power. As the paddle enters the water much of the effort is pushing down on the water. In the middle of the stroke you are pulling horizontally. And at the end of the stroke you are lifting up on the water. Mimic the motion sitting on a chair and you can visualize the arc and see the transfer of power.
Therefore, when somebody asks about the degree of bend in a paddle they are really asking, "is this the most efficient paddle you have?"
How do I measure for a bent-shaft paddle?
All other variables being equal, the bent-shaft will be about four inches shorter than the straight shaft paddle. The quick and dirty method is to sit on a bench, turn the paddle upside down, and shoot to get your nose right to the bend in the paddle. Other factors like seat height will affect the length, but this is the best place to start.
Which paddle is right for me?
Bent-shaft paddles work well for a canoe team that is going to paddle with the hit and switch method. Which is taking four to six strokes on one side of the boat and then switching to take four to six strokes on the other side of the boat.
If you are a j-stroker, like myself you may prefer the straight shaft paddle. I have found the twist of the j-stroke with a bent-shaft paddle to be a bit too cumbersome. Yeah, yeah, I know. All you bent-shaft converts have no problem with the j-stroke, I am just stating this old guy likes to j-stroke with the straight-shaft paddle.
What if I was to suggest this? How about a 12 degree bend for the bowman and a seven-degree bend for the sternman?
In the end it is a personal choice. And who really cares how you propel your boat, just get out on the water!
Photo and Article By: Brad Putney. "Captain" Brad Putney considers himself an inland waters guru after spending 40 years on the lakes of Minnesota. The Captain likes to say he has been at the helm of everything from a ten-foot inflatable to an America's Cup yacht. Mr. Putney can be found at the Duluth Pack Store, helpfully dispensing free advice. He has degrees from Gustavus Adolphus College and the University of Minnesota. Please contact at: email@example.com.


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The L.A. Times took the opportunity to come out against not just the federal expansion that is the U.S. Supreme Court's focus in Comstock, but civil commitment of sex offenders more broadly:
"Using the civil commitment process to lengthen a criminal sentence is dishonest and dangerous," cries the subhead of yesterday's editorial, "Sex offenders behind bars: How long?"
That issue of federalism isn't unimportant, but the more pressing question is whether civil commitment for a mental condition is being misused to force felons to remain in prison after they've completed their legal sentences.The Wall Street Journal, meanwhile, focused on a critical angle that I'm planning to blog more about soon -- the dangers to civil liberties inherent in expansion of civil commitment laws to other groups. Decrying the attempts by "feds [to] usurp another area of state law," the WSJ pointed out:
The implications go well beyond sex offenders…. If the Supreme Court reverses the lower court's decision, it will sanction the notion that nearly any appealing idea may be justified as necessary and proper. In other countries, loose detention laws give wide latitude to authorities to lock up any number of people who "threaten the public safety," including political prisoners. Maybe next the feds could force everyone in America to buy health insurance.The L.A. Times editorial is HERE. The Wall Street Journal editorial is HERE. And, for any of you who want to really immerse yourselves in the Comstock case, I recommend the Sex Crimes blog, which has an incredibly comprehensive page of resources on the case.


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A new logo and website have been revealed in the first stage of a project to transform a historic Derbyshire town.
Following a successful bid by Bolsover District Council for European funding, £750k of European Regional Development Funding was secured to rejuvenate Bolsover, boost local trade, and showcase the town to visitors.
The 'Discover Bolsover' team has today unveiled the town logo and website, as the first step in a campaign to highlight Bolsover as a destination, and build on the huge popularity of English Heritage's Bolsover Castle.
Sarah Townsend, local town trader from Castle Oven bakery, said, "It's great to see the town being given a new lease of life and exciting to see how things are shaping up since we heard the news about the investment."
"We're really pleased with the logo, which reflects our local heritage, while giving Bolsover a modern new look. I hope visitors to the town like it just as much as we do!"
Bolsover District Council's Cabinet Member for Regeneration, Councillor Alan Tomlinson said, "A lot of planning and development work has gone on behind the scenes over the last year that will create a new look which brings our rich local history to life but also shows off Bolsover as a destination for the 21st Century visitor."
"We are working hard with all the partners involved to get as much value as possible out of the funds, which were awarded for very specific purposes. We're delighted with the logo and are looking forward to seeing the town transformed over the next year."
The project is being led by a 'town team' which includes representatives from Bolsover District Council, Old Bolsover Town Council, stakeholders and businesses.
Marketing company Ruby Slippers Ltd has worked with the team to develop the logo and website, after being appointed in January to deliver the marketing aspects of the project.
The majority of the investment is being spent on town centre public realm improvements that will take place throughout 2014 along Castle Street and the Cenotaph area. The improvements will make the Cenotaph area a functional, attractive place for people to embrace and will entice visitors from the Castle to visit the town centre and the numerous shops on offer.
The funding is also being spent on signage and interpretation, a family events programme, marketing, social media and PR.
The £750k is made up of European funding, plus match funding from High Street Innovation Funding, Bolsover District Council and Old Bolsover Town Council.
The town centre improvements will take place within the Bolsover Conservation Area, which is home to a range of heritage assets covering a long period of history, including the Grade I listed Bolsover Castle and its Conduit Houses, the Grade II listed New Bolsover Model Village, and the Scheduled Ancient Monuments of the Town Entrenchments – a rare 12th Century 'city wall' type structure that still circles part of the town.
Dennis Skinner MP for Bolsover, said: "It's fantastic to see Bolsover being given proper investment that will really show off the best of what our town has to offer. The castle brings in thousands of visitors every year, and it's great to see the residents and traders being given a chance to make the most of this appeal. I'm watching the developments with interest, and hope all our visitors enjoy their time in brilliant Bolsover."
For more information about Discover Bolsover, visit www.discoverbolsover.co.uk, email email@example.com, follow us on Twitter @alloverbolsover or like our Discover Bolsover facebook page.
For press enquiries please contact Liz Close or Nichola Atherton at Ruby Slippers Ltd, on 07729 130957 / 0114 221 7487 or email email@example.com or firstname.lastname@example.com
The Discover Bolsover brand is part of the Bolsover town centre public realm and infrastructure project. The project is part financed by the European Regional Development Fund Programme 2007 to 2013. The Department for Communities and Local Government is the managing authority for the European Regional Development Fund Programme, which is one of the funds established by the European Commission to help local areas stimulate their economic development by investing in projects which will support local businesses and create jobs. For more information visit www.gov.uk/browse/business/funding-debt/european-regional-development-funding


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Entrepreneurial success begins in childhood.
All it takes is an astute parent to mentor and guide the child to discover they can make money using their talents. Since my last Business Tips article, based on the book "Young Bucks, How to Raise a Future Millionaire" by Troy Dunn, I've been searching my memory bank for examples. I'll share those examples as well as explore some Dunn has identified.
As a child, I was by and large the best at making fudge. I knew exactly how to test the "soft ball" stage, how long to let it cool once I'd added the butter and vanilla, and how the sheen on the fudge indicated the precise time to stop stirring and pour it into the buttered dish. My mother taught me all of this, but it was our neighbor, Mary Campbell, who bragged the most.
Every time I made it, I would deliver a few pieces to Mary. Every time she complimented me it made me want to do it again. Had my parents been exposed to the ideas Dunn writes about, I very well could have become a millionaire making and selling fudge. Unfortunately, I didn't parlay that talent into a million-dollar business, but I definitively know a great piece of fudge.
The second idea that could have made me rich was the use of a backpack as a book bag. Again, my mother had taught me how to sew. Using that knowledge, combined with a need to carry my books while riding my bicycle, I designed and sewed a book backpack complete with my initials on it. In the early '70s few high school students owned cars, we used bicycles. If my parents had encouraged entrepreneurship, I might be running my own factory producing Peggy's Packs.
The common thread in each of these examples is the "want." I like fudge therefore I wanted some and learned how to make it. I wanted a way to carry my books on my bicycle therefore I learned how to sew a backpack.
Give your children and grandchildren the "Gift of Want." Dunn says it best, "Giving them everything they could possibly want, and more, is no gift. All they could learn from our over generosity is to ask for more and appreciate what they had less."
Dunn challenges us all to create an opportunity for our children to "figure out a way to pay for what they want." If we exercise restraint in giving them everything, encourage the opportunity to create a business, then we are teaching them to "learn to earn."
Allowance without responsibility attached teaches children to depend on us for their income.
"Children need to learn as soon as they are able, that money is not a gift but a compensation for work done." They will in turn learn, "that earning their own money gives them the freedom to spend it any way they like," Dunn writes.
Dunn identifies ways for children to earn money. Even the youngest child, 5 and under, can learn to earn. Once I watched as my daughter stacked dimes on the corner of the kitchen cabinet. Each time she told her son to do a simple chore, she'd check to be sure it was done then the child was able to pick a dime off the stack. Instant gratification is important at this age.
For children ages 6 to 10 a simple lemonade stand can teach them to learn to earn. The younger child will only understand the simple math, but the older child can do the math to determine the cost of what is being sold. Personally, I never pass up a lemonade stand and I never pay more than asking price. Other tasks such as sweeping driveways for neighbors, picking up the neighbors' papers if they leave town or even taking care of pets can teach children about earning money. I remember an aunt who paid my brother and I five cents for each bag worm cocoon we picked from her evergreen bushes.
Children from 11 to 14 will most likely have their own ideas for a business. They may create "spirit" items to sell to their school friends, tutor younger children, and learn to fix bicycles or water container plants for a family friend on vacation.
The older group of children ages 15 to 18 will be ready to possibly launch a business. They no longer are satisfied with the simpler possibilities they had early on in their history of business. They may learn how to copy VHS tapes over to DVDs for a fee. Other entrepreneurs may create holiday wreaths or baskets or even detail cars. Explore their talents and help them turn that into a business.
Entrepreneurs fuel our economy. We all need to be doing what we can to assure the spirit of business ownership continues to prosper by encouraging children to "learn to earn."
"Business Tips" was written by Peggy Rosser, Rural Business Development Specialist and Certified Business Adviser IV of Angelo State University's Small Business Development Center. Contact her at firstname.lastname@example.com.


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EXCLUSIVE: Beyoncé Knowles has donned many guises in her entertainment career -- musical superstar, fashion icon, actress (yeah, we'll even count her "Pink Panther" turn).
Now she's looking to add a rather different notch: documentary director.
Knowles and her representatives at agency ICM have in recent weeks been shopping a nonfiction film to Hollywood studios about the celebrity's life and career.
PHOTOS: Hollywood back lot moments
According to a person familiar with the package who was not authorized to talk about it publicly, the movie is as a mix of music and personal study, blending concert footage with confessional interview. Knowles not only stars in the project but is directing it, and will also serve as a producer. About 20 minutes of footage has been shown to distributors.
A representative at Knowles' agency did not immediately reply to a request for comment.
Knowles certainly would have a lot to discuss in the doc. In the last 18 months, she's released a top-selling album ("4"), split from her longtime manager (her father), and had a baby, Blue Ivy Carter, with husband Jay-Z, among other noteworthy events.


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Redskins coach Mike Shanahan said Friday that quarterback Robert Griffin III has progressed all week and that he was "much better" on Friday, leaving him to be listed as questionable on the final injury report of the week.
"We'll get a chance to evaluate him [Saturday] and talk to the doctors tomorrow and make a decision," Shanahan said, via Rich Campbell of the Washington Times.
Shanahan has made sure that he's covered in the event that Griffin's sprained right knee doesn't allow him to go. Jay Glazer of FOX Sports reports that Redskins players have told him that Griffin only did individual drills in practice this week. Kirk Cousins has gotten all of the first team reps this week to prepare him in the event he starts against the Browns.
That said, Glazer also reports that Griffin "truly" will be a game-time decision on Sunday. Cousins needs the reps with the starters more than Griffin at this point in the season and Griffin was going to be limited anyway because of the knee injury.
And so the waiting game continues in Washington.


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Spurs fan Thomas Newman does what every football fan should do at least once in their life; take in three football matches in three days in Germany.......
FC Köln vs Hannover 96
'European Football Weekends is a perennial source of pleasure' The Guardian
Ah, the delightful stadium currency conundrum.
Many teams do it but just as many don't. St Pauli want to do it but their supporters are currently saying "no".
Part of it worked when I was at Augsburg - once I had my card there was no waiting around for a beer. But getting money added to the card was a pain.
To be fair if you're only going for one match its a pain but if you're a season ticket holder then it makes more sense as you don't have to mess about with money- you can just top up once every few games. Swings and roundabouts...
A great read. Yep the cards are pain. Trying to work out how much to put on them is never easy. I've lost count of the times i've stood at the bar, ordered two drinks and burgers and found i didn't have enough on the card.. because they whip off money for the glass deposit too.
Still, it's all part of the fun and I wouldn't miss it for the world! Did the Cologne Friday/ Schalke Saturday thing earlier this season and it was great!


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Yeah, I listened to both again, and I hear the difference now. Still, when I see the word "arrangement" I'm hoping for a bit more than just better quality sound. Secret of Mana music done with a full orchestra, perhaps? Not THAT would be awesome. Until then I happily continue listening to my OST, which is quite fantastic.


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"Very few professions require the kinds of demands of a pastor; they must be available 24/7," said Linda Pedersen, synod minister for the Southwest Minnesota synod of the ELCA.
Being a pastor is easy, right? After all, they really only work for a couple of hours Sunday morning.
While said in jest, these words poking fun at clergy could not be less reflective of the reality of serving as minister for a church.
"Very few professions require the kinds of demands of a pastor," said Linda Pedersen, synod minister for the Southwest Minnesota synod of the ELCA. "They must be available 24/7."
The inherent expectation pastors are supposed to be on call all of the time, day or night, is a role they are willing to fill, be-cause it comes with the role of ministry in a congregation.
Yet, people in congregations need to recognize what pastors are willing to do and to express their gratitude for what they do.
October is Clergy Appre-ciation Month, which encourages congregations to spend time honoring their pastor and the work being done by the pastor.
In addition to being on call when one of the members of the congregation needs them, pastors fill myriad roles in churches, especially in smaller, more rural congregations where the pastor is the only member of the staff.
There are days when a pastor may be called on to visit one of their flock who is in the hospital during one part of the day and be at the church later on to counsel someone who is struggling with their faith.
"Pastors offer comfort, care and provide that sense of safety for people," said Pedersen, adding at times they are working with others in the community, too.
The pastor in the community is considered a safe and trusted person, which can be demanding.
When someone is hurting, the pastor goes, said Pedersen.
While the role of the pastor can be a busy one as they are constantly working with people, it still can be a very lonely one.
After all, pastors who offer spiritual counseling and challenge members of the congregation to grow in their faith are not always able to establish outside of church relationships with people in the church.
Add to that the fact that pastors are called to communities which can be some distance from their family, and one can see how isolated pastors can feel as they serve.
"They need to have friendships, just like the rest of us," added Pedersen.
A congregation needs to encourage those kinds of relationships, because they are essential to the health of the pastor.
Church members also need to remember those pastors who are married and have a family must fill the role of leader for that family.
That means having time to be with his family and growing and nurturing relationships with their spouse and their children.
"Pastors are human, too," said Pedersen, adding it is dangerous for people to put their pastor on a pedestal.
While the month is nearing its end, there is still time for congregations to express their appreciation. What follows are a few ideas offered by Focus on the Family, which helped bring attention to the idea of clergy appreciation month back in 1994.
• Host a card shower with members of the church sending a card of appreciation to the pastor for his work.
• Provide a time for testimony during a church service or Sunday school hour when people can share how the pastor has helped them.
• Have a special dinner planned wheth-er it is for the pastor and their family or the whole congregation.
• Present some gift to the pastor. Rem-ember pastors do not serve in their roles to get rich.
• Pray for your pastor and offer words of appreciation to them throughout the year.


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American inaugural addresses often cover similar ground. They celebrate American values while looking forward to the future, but the vocabulary has changed over the decades. In his 1957 address, Eisenhower mentioned "dynamos" while discussing the prosperous industry. In 1989 George Bush likened cocaine to a "deadly bacteria". Bill Clinton, in the last inaugural address of the 20th century, declared America's children would be safe from "nuclear, chemical or biological weapons".
The following graphs visualize inaugural addresses words. Each dot represents one word. Darker dots indicate more frequent usage. The graph radius represents duration of speech. Common English words have been removed.
DATA: Miller Center
Gabriel Florit / Globe Staff


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Behind the Scenes: Minardi and Garrison on Their July 2013 Cover
Beth Minardi and Mark Garrison joined forces to create a beautiful finish for our July issue. The duo requested a non traditional model for their Modern session. "I wanted to do something sexy," says Minardi. "I wanted to show how we could take a dark, heavy look and lighten it up for summer."
For color, Minardi created what she calls a "modern approach to ombre" placing golden, peachy highlights around the face and along the ends. "What I try to do is produce texture with haircolor," she says. "My zig zag partings and tight angles around the face allow for a flattering flow of color. For shade selection I create shades that are not necessarily natural, but what I think are better than natural... in this case warm, spicy and sexy."
For Cut, Mark Garrison chose to use his signature layer cut to complement Anastasia's face shape and type. "I gave her a classic model cut," he says. "It's simply a layered cut, with a face frame blended down from the chin to the length. This cut is great for all models AND clients, and works on just about hair texture."
In this short video, Minardi and Garrison break down the look, offering the haircolor formula and more detail about the cut.


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:: Student-Athlete Study Hall
All freshmen and new transfer student-athletes are required to attend 6 hours of study hall per week during their first academic year at Georgia Southwestern State University. All other student-athletes with a GPA below a 2.5 are required to attend 6 hours of study hall, while those below a 2.0 are required to attend 8 hours per week. If a student-athlete earns a cumulative GPA above a 2.5 they are no longer required to attend study hall, unless dropping below a cumulative GPA of a 2.5 at a later date. Each head coach can increase the requirements for study hall at his/her discretion.
All study hall hours will be logged in the Academic Resource Center (ARC), located in Collum Hall on the First Floor. ARC hours are from 9:00 AM until 7:00 PM Monday-Thursday and 9:00 AM until 3:00 PM on Friday. Student-athletes can earn study hall hours at any time the ARC is open.
Student-athletes will recorded their study hall hours in the sign-in book located the ARC, as well keep as personal log sheets which will be submitted to the Head Coach at the end of each week. A study hall "week" will start on Tuesday and end on Monday.
Penalties for Missed Study Hall Hours
To ensure our student-athlete's attendance at the required number of hours each week, the following penalties will be in place for missed study hall hours.
1. First Offense - The penalty will be at the discretion of the coach with a rollover system in place. The hours missed for the previous week must be achieved the subsequent week. For example, if a student-athlete is required to have 6 study hall hours for the week and only earns 5, the student-athlete must obtain 7 the following week or a second offense will occur.
2. Second Offense - Be withheld from one practice (+2 hours of study hall)
3. Third Offense - Be withheld from two practices (+4 hours of study hall)
4. Fourth Offense - Total disregard for study hall policies and procedures. Student-athlete will be withheld from one competition. If competition is not an option, the student-athlete will be withheld from five (5) practice days.
In the case of extreme and extenuating circumstances that prevent a student-athlete from completing their required hours, the student-athlete must meet with the Compliance Coordinator or Assistant AD. If it can be determined that the circumstances are extreme and extenuating, an exception to the penalty will be granted.
NOTE: Poor planning and negligence on the part of a student-athlete will not constitute extreme or extenuating circumstances.
Study Hall Policies
While in study hall, all student-athletes must abide by the following policies. If a student-athlete fails to follow these policies, the student-athlete will be asked to leave study hall and lose any hours earned during that particular study hall session.
1. Sign in when you enter the ARC. Sign out when you leave. Failure to do so will prevent you from receiving credit for any hours earned.
2. DO NOT sign in or out for anyone but yourself. Be prepared to show your student I.D.
3. No food or drink in computer labs.
4. Keep talking to a low level. Study hall time is not for socializing.
5. Be courteous to other students.
6. No cell phones at any time. Cell phones should be turned off or left in your room.
7. Students must use study hall time to complete academic coursework. Study hall hours may not be earned while internet surfing, doodling, listening to your IPOD, etc. If you think you do not have academic work to do, read your textbook, reread your textbook, start your next assignment, etc.
8. Computer Lab: No internet surfing. The internet must be used for academic purposes. This means NO email and NO personal/social pages such as Facebook or MySpace. The study hall coordinator or any other Athletics Department personnel may question the purpose of a student-athlete's use of the internet at any time and the student-athlete must provide an adequate explanation.
9. Computer Lab: No instant messenger of any kind (i.e. AOL, Yahoo, MSN, etc). This includes having an instant messenger program open and/or active regardless of any conversation.
10. Computer Lab: Do not print any document without permission from the lab assistant. Do not print emails at any time.
11. Computer Lab: Do not add or change desktop background or icons.
12. Computer Lab: Do not save any files to the computer.
13. Computer Lab: Do not run, download, or install personal applications.
15. ARC supervisors have the authority to enforce these policies as they see fit. If you cannot adhere to the rules, you will be asked to leave and any hours completed during that time will be lost. If you are asked to leave the ARC, an Incident Report - bearing your name and details of the infraction - will be submitted to the Head Coach as well as the Compliance Coordinator.