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February 15, 2012
Naturally, some foreign parents will have babies born in the CNMI, including foreign contract workers who consider the CNMI their home after spending most of their adult lives working and living in the islands. However, the foreigners who are coming to Saipan for the purpose of delivering babies that will be granted immediate U.S. citizenship are in no way a part of the community. They will return to China or Korea after the babies are delivered at CHC.
The CNMI Delegate, the Governor, and some vocal Republicans in the U.S. Congress (if we are to believe the spin) do not want the legal, long-term foreign contract workers to be given green cards or a pathway to citizenship even though for decades they have contributed so much to the CNMI community. Not one CNMI official has come out to discuss the fact that Saipan has become a backdoor for U.S. citizenship for Chinese tourists, who are strangers who will drop some dollars for a month or two, give birth in the CNMI to create instant U.S. citizens and leave. Perhaps they are silent on the subject because they fear that the visa waivers will disappear if the federal officials hear about this.
The websites that are geared to pregnant Chinese and Korean women, saipanbaby.com known as Kelly and saipanbb.com known as Saipan Children Services Agency, give the impression that the CNMI government is aware of and encouraging this practice. According to both websites the Commonwealth Health Center, the government-run hospital, is involved in booking hospital rooms, setting up prenatal appointments, arranging for the attending physicians and all other hospital-related arrangements for the Chinese mothers. All arrangements from travel, to accommodations, hospital appointments, and securing the newborn's social security card and passport are arranged by the baby tourism companies who charge between $9,775 and about $11,000 per woman. They are coordinating their efforts with multiple people on Saipan.
One person featured on the Saipanbb.com website is former CNMI Immigration Director Melvin Gray. There is a photo of him and the text on the site reads (roughly translated from Chinese):
To have children on Saipan, Saipan government department's advice:
Melvin Grey photo on Saipanbb.com
Former Saipan Director of Immigration, Melvin Mr. Grey
In accordance with the provisions of the 14th Amendment of the Constitution of the United States, the United States to implement the birthplace principle, as long as the child born on U.S. soil, regardless of their parents are not U.S. citizens, the child shall be a U.S. citizen. . .
In 2008, the Saipan Director of Immigration, Melvin Grey said to help foreign women to the state to have children within this approach is fairly common, but in accordance with the provisions of the Fourteenth Amendment, the state or the United States Federal Immigration Service cannot do anything.
He said: "This does not belong to illegal activities. The United States has this problem."
He said U.S. immigration officials sometimes ask a pregnant woman to prove that during her stay in the local payment of the corresponding expenditure, enter What is the purpose in the United States, but as long as these women can produce relevant evidence, completely legal immigration.
He said: "The United States immigration officials is difficult to deal with these things, because women generally come to visit friends, do not stay too long."
He also said that the local immigration officials generally do not ask the purpose of entry of local pregnant women.
He said: "We do not want to ask these questions, the lady of this will bring pressure." He added that if you exert too much pressure, unforeseen circumstances may cause women to have abortions, and if that happens is a serious violation of humanitarian principles.I omitted confusing text about abortion. You can click on the PDFs below to enlarge and read:
It isn't clear if Melvin Grey is involved in bringing the women to the CNMI, if he endorses the site, if he actually stated the words that were attributed to him, if he profits from the baby tourism business, or if he even knows he is featured on the site. But he is featured on the site and that alone is damning.
It is clear that there are people in the CNMI who are profiting off of the baby tourism business. These people include the hotels, guest houses or apartments where the women stay; those that are serving as "guides"and travel agents; those that are serving as the private nannies for the babies; those who are collecting fees for the hospital services and those delivering the babies. Is this one of the ways that CHC plans to recover from bankruptcy? What do Juan Babauta and the hospital physicians have to say about the "several hundred women a year" who deliver babies at the CHC to attain U.S. citizenship?
The Saipan contact number listed on the website is 670-235-2335. Who is this person? One hotel featured and promoted on the Saipan baby site is the Aquarius. Photos of the apartments and guesthouses are also featured on the sites.
Previously I reported on this site about the CNMI-Korean baby tourist industry in Saipan and Guam. In July 2008, I wrote:
For quite some time Saipan has been advertised in Korea as a place for pregnant women to deliver their babies in order for them to receive US citizenship. Last year I discovered that Korean agencies were teamed up with individuals in Saipan to arrange for the mothers' stay at a Saipan hotel, coordinate the hospital and delivery details, and process passports in package deals like any other vacation packages. The pregnant mothers arrive as tourists and return to Korea with their new US citizen babies.
Now the media is reporting that the scheme has spread to China. A July 12, 2008 article from The New American Media reports:The Consolidated Natural Resources Act 2008, which was signed into law by President Bush in May, has become a springboard for Chinese nationals to immigrate to the United States, reports the World Journal. The Act enables the United States to take over the labor and immigration system of Saipan, the largest island and capital of the U.S. Commonwealth of the Northern Mariana Islands (CNMI). Lawyers say that employers in Saipan are allowed to apply for U.S. work visas for immigrant workers under the new act, and that babies born in Saipan automatically be U.S. citizens. Many Chinese are moving to take advantage of the act, the World Journal reports. Immigration services in big cities like Beijing, Shanghai, Guangzhou and Wuhan have started programs like "Labor Export to Saipan" and "Deliver an American Baby in Saipan." Expecting mothers pay anywhere from 140,000 to 240,000 RMB (about 20,000 to 35,000 USD) for the service that will allow them to deliver their babies in Saipan.
One agency that specialises in helping mainlanders deliver babies in Saipan, a US island, charges just 70,000 yuan for round-trip tickets to the island, medical services and two months' food and accommodation. The island has not required visas for Chinese tourists since 2009, and the agency claims people born there automatically get US citizenship.Saipan Baby (Kelly) Goes After Saipan Tribune
The staffer said several hundred mainland women had flown to the island each year since 2009 to have their babies.
|Click PDF to read page
KELLY'S DECLARATION ABOUT THE the REPORTThis extremely unprofessional rant may get the baby tourism business more attention than they expected. Maybe their statement against the Tribune will get the attention of the U.S. Departments of Homeland Security, State and Justice. The "nobody can interfere in our business" line may be wrong. Does this business have a business license in the CNMI? Who are the owners?
Yesterday I known that the Saipan Tribune had reported us. The charge, process,
policy and so on. I'm so surprised that nothing can be done.The newspaper and
the reporter are too free and have nothing to do.
Our clients like delivering baby in Saipan.We offer services. That 's equal. They pay. We service. They give us what we want. We give them what they want.
Nothing to discuss. If somebody be angry or dislike, that's his problem of himself. Nobody can interfere our business.
The reporter, listen! You should pay more attention to public security, local
of economy depression and bad human and culture environment in. Saipan.
You should pay more attention to the whores be heisted in Garapan on
daytime.You should pay more attention to marriage illegal cause it now
become worse and worse in this small island.You should pay more attention
to the society contradictions becoming increasingly acute but not us.You are
wasting your time, wasting the newspaper's page and also wasting our time.
The reporter, look! I have a good idea to you: Call your government to revise
the law. Then nobody can get the citizenship by giving birth there.If you can,
just do it. If you can not, shut up and get out!
Finally, next time if you want to report us better call me first and the request
my agreement.Better in Chinese cause my English is just soso.If in Chinese,
I can correct you in some place.You have a lot of mistakes in the knowledge.
Next time if we have chance better we sit together and I will teach you
something. Our business is a complicated article and technique.
Not so the simple as you think!
Certainly the readers of the website who are considering having a baby in Saipan will think twice when they read about "whores heisted in Garapan" in the day. What tourist, whether a vacationer or visitor intending to give birth, wants to go to an island like that!?
It would be great if Haidee or another reporter interviewed Melvin Grey, Juan Babauta and others to see what they think of this baby tourist industry.
Several hundred women paying cash at CHC each year to deliver babies must contribute to the cash flow problems, which may be why the practice is ignored by CNMI officials.
(saipanbaby.com) website that outlines the benefits:
"You may not know, the United States is an island - Northern Mariana Islands, Chinese visitors to the purposes of visa-free, it is Saipan. Have been countless mothers across the oceans have a child in Saipan, and American identity for the children each year. Mothers who intend to go to Saipan for birth confinement Saipan, the USA sub-center looks forward to you and your baby in the United States Saipan warm home. We offer a complete, labor and postpartum services, your month of homey feeling, so that mothers can sit comfortably in Saipan. . .
. . . lets you easily into the United States give birth, and at the end of the month you can hold a U.S. passport and you and the baby return home. Life of the United States to give birth to the child an identity, let her (him) win at the starting point, this is Mom and Dad led its left baby millions of family property, might as well give him (or her) a United States citizen identity, in the future Chinese competition on stage, your baby has won at the starting point!
Kelly month sub-center is located between the mountains, around the living environment is quiet and elegant. The house is a villa-style apartment buildings, bright and spacious. Here you can enjoy the resort's family life, we are Saipan only a family-month sub-center, non-intermediary organizations. Many apply for agency expertise and handle the lack of experience caused great losses to our customers, including the charge of a large number of intermediary fees, pregnant women refused entry by customs, visas adverse records, accommodation and the description does not match. Many companies are now mixed fish heads, good and bad, we solemnly declare: all-inclusive of the 70,000 yuan in our children! Includes: air tickets, birth, stay two months, meals, document processing, hospital escort, meal delivery, Yuesao care, transportation, translation, and all costs! Let your baby successfully get a U.S. passport! ! Also lay a solid foundation for your future family moved to the United States! !
Became a U.S. citizen. Benefits: Your child will immediately have U.S. citizenship, the enjoyment of civil rights; 21 years of age, parents and siblings can apply for dependents, with a permanent green card. No need to wait for the quota, future immigration conditions.
U.S. citizen children attending U.S. public elementary school to high school, and completely free tuition. The university can enjoy a tuition discount of U.S. citizens, and student loans. U.S. citizens attending public universities, the Institute of tuition as long as a fraction of the foreign students, preferential access to the well-known universities. U.S. citizens enjoy generous scholarship of the university, but also enjoy very low interest rate student loans. U.S. citizens can serve as priority the U.S. government, government agencies and large U.S. companies important leadership positions.
U.S. citizens to enjoy more than 180 diplomatic allies worldwide visa-free entry, the most favorable entry and exit convenient seven U.S. citizens enjoy generous social welfare and advanced medical equipment. . . Kelly month sub-center is you and your child's best friend in Saipan, full service only 70,000 yuan, including: air tickets, birth, stay two months, meals, document processing, hospitals escort, meal delivery, Yuesao care, transportation, translation, and all costs! Record the lowest price, so you get the most satisfying benefits with the lowest cost! Domestic contract booking and pay a small deposit, most of the costs paid to enter the island, more at ease, more at ease! Kelly month of center I wish every mother's dream come true!The services offered by the baby tourism sites include "clearance training" for getting past immigration officials. (Why would they have to get past immigration?) The site states (roughly translated from Chinese):
We will provide you with a Kelly pass secrets (fine, containing eight chapters eight method) and carefully prepared. Prior to departure, you are requested video with us, we need to see pregnant women at the border of clothing, wearing, and pregnant women, strict immigration and customs training, pass the training time for about two hours. International roaming for your phone, to the island contact. Do not bring any clothes for the baby and delivery or supplies and to ensure that the luggage.The site also tells how the mother will be helped at the hospital and to get U.S. documents for the newborn:
The center will create a profile in a private hospital for check and check-ups include: blood tests, routine examination, B-ultrasound, fetal heart rate monitoring, and other private doctor delivered or surgeon. . .delivery in large hospital. Throughout the delivery process, the sub-center female interpreter will accompany the client. A Yuesao (nanny) will be provided in the hospital to take care of the newborn, the center staff will deliver meals for mothers when discharged through the discharge formalities and apply for baby of the birth certificate.The question and answer section of the website also has some interesting questions and responses.
After the birth certificate is issued the center staff goes to the Capitol Hill passport office to apply for baby passport (Urgent, 1-3 weeks desirable), and for baby SS card (take cards) and baby within a month travel permit (a month).
I wonder what impact, if any, this revelation will have on the Department of Homeland Security's generous policy of issuing Russian and Chinese visa waivers to Saipan.
Governor Fitial and AG Buckingham are off to Washington, DC again on the latest junket. Undoubtedly their agenda will include meeting with their highly paid attorneys and consultants to push the lawsuit that they filed against the U.S. Government over Social Security being applied to the foreign workers.
High on their agenda will also most likely be meetings with DHS, Fitial's Republican allies and others to convince them to follow the points in the other lawsuit against DHS and USCIS that Buckingham threatened to file in January. Buckingham announced that DHS and USCIS were meeting their demands after several conference calls so the lawsuit has been called off. It appears that the Fitial Administration wants to "work with" ICE and USCIS to review any lists of "overstayers" so that they can ensure deportation of any person that they want to leave. For instance those who have had wages stolen by political allies and the like.


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subset: commoncrawl


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J. Fred Muggs was the most celebrated of all TV chimps. Innovative, charismatic, trailblazing: Muggs set the standard for performing simians worldwide. This isn't his story. We' re here to discuss the ape that eclipsed him. A talking ape.
Kokomo Jr. was the first and probably the only talking chimp to come down the pike. Likewise, the first chimp to ski down a mountain, first chimp to open a checking account, first chimp with a syndicated newspaper column, first chimp on a Hallmark greeting card - you get the idea. In the 50s and 60s, he swung on the brass ring of show biz success to the accolades of humans everywhere. He rubbed hairy elbows with Presidents and First Ladies, comedians and clowns, singers and schpritzers. Kokomo spent a fair amount of time making with the personal appearances and fundraising for various charities. He also emerged at elementary schools across the land, doing tricks and teaching civic lessons to the kiddies. Can anyone truly calculate the lasting effect dapper Kokomo Jr. had on the slack-jawed youngsters? Off the clock, his swinging coterie included the likes of Julie Newmar and Buddy Hackett. The world was his banana and he peeled it in style.
Currently retired in North Carolina, Kokomo tools his Big Wheel around the neighborhood and auctions off his paintings on the internet. What a simple, naked existence Kokomo would have endured had he not crossed paths with his future pal and confidant Nick Carrado. Back in the heady days of '55, Carrado was an ex-marine eking out several careers in the insanity that was and still is New York City. By day, he laid bricks for skyscrapers. Between 5 and 7pm, he taught self defense and martial arts to NYC's Finest. Sometimes he'd ride around with the detectives and watch his teachings put into action. In the wee hours, Carrado pursued his childhood dream of prestidigitation in nightclubs and swank Italian restaurants. A little passing the coin through the table, some slight-of-hand and the ol' rabbit out of the hat. Carrado created a buzz that landed him out-of-town gigs when time permitted.
Into the spotlight swung a talented little fella, destined to change the act forever. In a recent conversation, Carrado recounted Kokomo's rise to fame and also a surprising revelation about a certain hairy doppleganger. Here is their story, in the words of the man who knows it best.
"One time I was performing up at Blimstrom's, which was the largest night spot in Boston. I stopped at a wild animal farm up on the Cape, and that's where I first saw Kokomo. I just went goo-goo over him! He was about a year old when I met him. Probably 1956. The thing was, I was tired of producing a rabbit out of my hat. The rabbit was really dumb and I could never teach it any tricks and I said, 'Wouldn't it be great to get a chimp and produce him not out of a hat, but out of a foulard - a large silk. Then I could tell him to disappear and then go on with the act!' The problem was, he would clown around while I was doing my sleight of hand, which I took very seriously. The people would start laughing like crazy. So I said, 'Look Kokomo, either I'm gonna do the act or you're gonna do the act!' I guess you know who won out!
I named him Kokomo Jr. after two of my Marine buddies who never made it back. It was 'Coco' and 'Moe' and I was the 'Jr.' Now, Kokomo didn't do tricks, per se. He was understanding and very understanding of words. Riding a bicycle, roller skating...any chimp could do that. I would teach him 'Open!' Like open a box, or open a door or close a door. Anything with open or close he was a natural at. Once you start teaching the basics - like open, close, pick-up, put-down, put-back - a lot of things he could easily do. I was a single fella and we had a good rapport going. I spent plenty of time literally raising him like a child.
He used to do magic tricks. He would do box magic which you don't have to be too brilliant to do. We had a bit where he would pour water in a vase, wave his magic wand then pull a never-ending foulard out of it. He'd stop in the middle and give the audience a look like, 'Is this ever gonna end?' Oh, they loved it!
I put the show on the road. I went down to Florida with five dollars in my pocket. Just enough to feed a chimp. Sometimes in the trailer parks, I'd send him up a tree to get a couple of coconuts. Then I heard that the Today Show people were doing a remote down there. They wanted to take a look at Kokomo, as they had heard a lot about him. This was after J. Fred Muggs left the Today Show. Muggs was the granddaddy of all TV chimps. He was great. Kokomo Jr. and Muggs never did meet, though. Anyhow, NBC taped the whole show around us and we were hired on the spot.
Koko was on the Today Show with Dave Garroway everyday for a couple of years. He'd do the weather and lots of funny little bits. Sometimes the producers would request special routines. They would call me and say, 'Hey, can we have Kokomo play the violin?' and I'd say, 'Sure.' A few weeks later, there he is sawing out a tune.
He always had a huge wardrobe. Brooks Brothers suits, cowboy outfits, marine uniforms, a leopard skin lounging robe. The tailors would cut the pants shorter, the sleeves a little longer and he was good to go. I used to take him for a shave and a haircut once a week. He loved it. He also ate with a knife and fork.
In his heyday, he was on most of the big TV shows: What's my Line, I' ve Got a Secret, To Tell the Truth - he done all those shows. We worked with some big celebrities: Bill Cullen, Charlie Weaver, Jake LaMotta, Jack Paar, Merv Griffin - worked with them all. He was a regular on the Howdy Doody Show for over a year. One time on Candid Camera they had him in a dry cleaner's store. He took the tickets and brought back the clothing and some people didn't even notice that a chimp was waiting on them! That's New York for you.
Teaching him to talk, well, that was difficult. I took him to psychologists, speech therapists, they all told me,' Chimps just don't talk.' I spent about four months getting him to make a sound with his vocal cords. Then another three months to make him shape his mouth, lips, and jaws. I found that he couldn't make the 'M' sound without overlapping his upper lip with his lower lip by about a half an inch. When he said' Mama' on the Today Show, it was all worth it. The Daily News gave us a full page, the Associated Press, you name it. One time a reporter challenged me on it, so I said, 'I'll come down there and I'll show ya,' which I did! I could've taught Koko to say more words. I have no doubt in my mind about that. I was busy trying to earn a living and I didn't have the time it would take to do that. It could get a little hectic.
You gotta remember we were on live TV back then. You didn't get no second chance. By the time we shot the Kokomo Jr. show on film everyone called him 'One Take Koko.' Tell him what to do one time, run him through once, and he knew it. A lot of the actors would blow it and have to do it again and again. Koko'd come over to me and be like, 'Man, I'm not gonna do this scene one more time!'
At the '64 World's Fair, Kodak used Kokomo on what at that time was the largest photograph in the world. They were looking for a chimp that played baseball and I said,' I' ve got two chimps that work together. They never played baseball before but I can teach them.' See, I' ve always had two chimps. I would rotate them between shows so they wouldn't get burned out or tired. I was always concerned about not pushing them too hard. You know - it's like Lassie. Some Lassies would run through smoke and fire, some wouldn't. The chimps are both named Kokomo. They know which one I'm talking to. One's Kokomo Jr and the other one I say, 'Hey Koko Koko.' It's all in the inflection of the voice. It's been seventeen years since we performed, so I guess we don't need to keep this a secret anymore. In the end, I'd rather that people know that I'm humane.
They both like to sleep in. My wife is an early riser. We run a company called Monkey Packaging Tape. They like to watch TV and flip through National Geographic. We have an island in the backyard where they run out to play. I don't dress them up any more. Usually if they go out on the island, I'll put red sweaters on them so I can keep an eye on them from a distance. Only a couple of hours and then they're back in. They like the air conditioning. They got the refrigerator - they love to snack all day. Chimps really like to make a big deal out of eating. When they' re in the jungle, they'll eat all day long. They'll travel and pick a little here, pick on this berry, what have you.
They like biking around on Big Wheels. That's something they do for pleasure. They've gotten more interested in painting lately. I leave the brushes and acrylics out in their room, and they'll just go over and start painting. Always nice to have a hobby."
To find out more about Kokomo Jr. or to see his paintings, go to: http://www.kokomojr-tvchimp.com.
° ° °
To find out more about either one of the Dave the Spazzes, tune in every Thursday night at 8pm.
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© 2000 WFMU. All rights reserved.


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Mini Golf Returns to Minnesota's Walker Art Museum
MINNEAPOLIS (AP) - Golfers can tee up again on the Walker Art Center's new artist-designed miniature golf course in Minneapolis.
The Walker has brought back mini golf to celebrate the 25th anniversary of the Minneapolis Sculpture Garden this summer.
But it's not your traditional mini golf course. This course features garden gnomes masquerading as foosball strikers, a scale model of a French chateau and gopher holes.
Winning proposals for the two eight-hole courses came from a group that included architects, artists and engineers. Two more holes were designed by students from a University of Minnesota public sculpture class.
Walker on the Green is open seven days a week through Sept. 8.


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The LanthaScreen TR-FRET assays can be performed on a variety of plate readers, including those from Tecan (ULTRA, Infinite F-500, Infinite\ M1000, and Safire2), Molecular Devices (Analyst), BMG LABTECH (PHERAstar Plus), and PerkinElmer (EnVision, Victor, and ViewLux) or any other plate reader configured for LANCE or HTRF assays. Monochromator-based instruments such as the SpectraMax M5 Multi-Mode Microplate Reader or Safire2 may not be suitable for some of the LanthaScreen Kinase Binding Assays with smaller assay windows.
Proper instrument setup is crucial for successful LanthaScreen assays. For instrument-specific setup guides, please see our step-by-step setup documents. Recommendations differ slightly among instruments; check the details carefully, particularly with regard to filter bandpass and recommended dichroic mirrors.
Settings common to all Eu or Tb LanthaScreen assays
Note: These settings differ from those for other commercially available TR-FRET assays and are optimized for LanthaScreen assays.
|340 nm (30 nm bandwidth)
|Delay time
|100 μs
|Integration time
|200 μs
For additional assistance or more information on performing these assays, contact your Invitrogen representative or Invitrogen Discovery Sciences Technical Support at +1 760 602 6500 (select option 3 and enter 40266), or email firstname.lastname@example.com.
Our calculations use the equation V1C1 = V2C2, where V denotes volume, C denotes concentration, and the numbers 1 and 2 denote initial and final conditions, respectively.
An important conversion: The number of μg equal to the molecular weight expressed in kDa is equal to 1 nmol.
|Molecular weight in kDa
|LanthaScreen antibody
|150 kDa
|1 nmol = 150 μg
|62.3 kDa
|1 nmol = 62.3 μg
|130.9 kDa
|1 nmol = 130.9 μg
|Fluorescein-CREBtide substrate
|2.076 kDa
|1 nmol = 2.076 μg
Epitope tags such as GST and His are always considered when calculating the molecular weight of a kinase. For any kinase complexes (e.g., cyclin/CDK dimer), the molecular weight of the complex should be used. For example, the molecular weights of CDK9 and cyclin K are 46.9 kDa and 44.9 kDa, respectively, for a sum of 91.8 kDa. The 91.8 kDa value is used to convert mg/mL to nM.
The V1C1 = V2C2 equation describes how the amount of material in a solution is the same before and after a dilution is performed. The amount can be represented as a mass (μg, ng, etc.) or in moles (μmol, nmol, etc.).
Kinases from Invitrogen are typically supplied at 0.1 to 0.5 mg/mL. To determine how much kinase to use in the binding assay, you must first convert the concentration in mg/mL to nM. The molecular weight is listed on the kinase Certificate of Analysis (COA).
Note: If you are not familiar with this equation, learn more by entering "V1C1 = V2C2" as the query in your favorite search engine.
Note: COAs are also available on the product purchase page. Enter the part number or kinase name in the red search box at the top of any Invitrogen web page. Select the specifications tab. Click on Certificates of Analysis and then click search (without entering a lot number).
Example: Src kinase
Src has a molecular weight of 62.3 kDa. Therefore, 62.3 μg = 1 nmol.
If the concentration listed on the tube for lot 26726QQ is 0.58 mg/mL, the conversion is:
0.58 mg x 1,000 μg x 1 nmol x 1,000 mL = 9,300 nM
mL mg 62.3 μg L
Using V1C1 = V2C2, calculate the volume of kinase needed to prepare the dilution.
For example, to prepare 1,000 μL of 15 nM Src kinase solution:
V1 x 9,300 nM = 1,000 μL x 15 nM; therefore, V1 = 1.6 μL
Because it is difficult to accurately pipet less than 2.0 μL of any kinase solution prepared in glycerol, we recommend preparing an intermediate 1:10 dilution using 1X kinase reaction buffer. Remember to recalculate the volume required for the diluted concentration of the kinase.
For example, dilute 2 μL of kinase with 18 μL of 1X Kinase Buffer A.
Perform the calculations again using a 1:10 dilution of kinase, so 930 nM instead of 9,300 nM:
V1 x 930 nM = 1,000 μL x 15 nM; therefore, V1 = 16.1 μL
Take 16.1 μL of diluted kinase to a final volume of 1,000 μL to prepare a 15 nM solution of kinase (which includes antibody for the binding assay).
Keep all diluted kinases on ice.
Note: Antibodies to GST or His tags are generically referred to as anti–epitope tag antibodies.
All LanthaScreen antibodies are 150 kDa. The Tb or Eu chelate adds an insignificant mass, so it is not necessary to consider their molecular weight. Since all antibodies are 150 kDa, the following general guidelines apply. Check the tube to determine the antibody concentrations. Standard LanthaScreen antibody concentrations are:
1.0 mg = 6.7 μM = 6,700 nM
0.5 mg = 3.3 μM = 3,300 nM
0.25 = 1.7 μM = 1,700 nM
Peptide substrate
Many fluorescein-labeled peptides are supplied at 1 mg/mL and must be converted to nM to determine how much to use in an assay. This conversion requires the molecular weight, which is supplied in the Certificate of Analysis (COA). For example, Fl-CREBtide (PV3508) has a molecular weight of 2,076 Da.
2,076 Da = 2.076 kDa; therefore, 1 nmol = 2.076 μg
1 mg x 1,000 μg x 1 nmol x 1,000 mL x 1 μmol = 9,300 nM
mL mg 2.076 μg L 1,000 nmol
The concentration of the fluorescein-CREBtide substrate is 480 μM.
LanthaScreen assays are addition-only assays; nothing is ever removed from an assay well. You add reagent 1, then reagent 2, etc. Each subsequent addition dilutes each other reagent. Remember to account for this dilution while preparing components.
For example:
|Volume added
|Fraction of total volume
|Prepared at
|Reagent A
|2.5 μL
|Reagent B
|5.0 μL
|Reagent C
|2.5 μL
The term 1X refers to the final concentration of a component for a particular reaction; a reagent prepared at 4X strength refers to the extent of dilution (1:4) required to achieve the final concentration in the assay.
Creating the 100X compound dilution series
A dilution series of test compound or control inhibitor in 100% DMSO is first prepared at 100 times the concentrations to be assayed. By performing the initial dilutions in 100% DMSO, you can minimize solubility problems associated with dilutions into aqueous buffer and stabilize the IC50 values.
For the control inhibitor and test compounds, we recommend 10- to 16-point curves with serial dilution steps ranging from a factor of 4 (4-fold) to a factor of 2 (2-fold). Fewer points or higher-fold dilutions are not recommended. Example of a 16-point 2-fold dilution series: A "master" dilution series of control inhibitor or test compound can be prepared in two separate 8-tube PCR strips (or in a 96-well plate), and stored at –20°C or –80°C for use in future experiments. The dilutions are "staggered" between strips, as shown on the left side of Figure 5. Two strips are used to facilitate the eventual transfer from a 96-well plate to a 384-well plate.
- Add 50 μL of DMSO to tubes 2–8 of strip A and all tubes of strip B.
- Add 100 μL of control inhibitor or test compound in DMSO at 100X the highest concentration to be tested to tube 1 of strip A.
- Transfer 50 μL of inhibitor from tube 1 of strip A to tube 1 of strip B.
- After mixing, transfer 50 μL from tube 1 of strip B to tube 2 of strip A.
- This process is repeated for all but the final tube of strip B, which contains only DMSO (no inhibitor). Discard 50 μL from the last well (A8) containing inhibitor, so that each well contains an equal volume.
Creating intermediate dilutions
Note: Intermediate dilutions are required to keep the DMSO concentration constant at 1% across the dilution series.
An intermediate dilution from the 100X dilution series is necessary prior to performing a LanthaScreen Kinase Assay. Test compounds or control inhibitors for the LanthaScreen Activity Assay are diluted to 4X, and for the LanthaScreen® Eu Kinase Binding Assay diluted to 3X.
Intermediate dilutions of test compound or control inhibitor are prepared in 1X kinase buffer plus any additives for the LanthaScreen Activity Assay. Kinase Buffer A diluted to 1X consists of 50 mM HEPES, pH 7.5, 10 mM MgCl2, 1 mM EGTA, 0.01% Brij-35.
To prepare these dilutions manually, we recommend volumes of no less than 4 μL for pipetting accuracy. See each assay section for more details.
Figure 5. Preparing a 100X dilution series of test compounds or control inhibitor. A typical starting concentration in A1 is 1 mM. Dilutions are performed in 100% DMSO.
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New Laguna Coupe launches with seven versions across two trim levels: Coupe and Coupe GT, priced from 20,995 to 27,995.
Entry-level petrol version, Coupe 2.0 Turbo 205, undercuts equivalent BMW 325i SE Coupe by considerable 6,805, plus 1,000 more equipment
High standard equipment levels including: 18" alloy wheels, Arkamys 3D Sound 140W audio system with MP3 CD reader, eight airbags, automatic lights and wipers, dual zone climate control, rear parking sensors and xenon headlights.
Top-of-the-range Coupe GT specification adds: 4Control chassis, automatic parking brake, electrically heated and folding door mirrors, leather upholstery and Renault Hands Free keycard.
Wealth of options for luxury customization: BOSE sound system, full color 3D satellite navigation with Bluetooth and 230W audio system MP3 6CD changer (in-dash), heated front seats with driver's side electrically adjustable with memory and tire pressure monitor.
Four engines at launch - two petrol and two diesels, including new 2.0 dCi 180 FAP and 3.5 V6 240. 3.0 V6 dCi 235 FAP to follow in early 2009.
Available to order mid-November and in dealerships 2nd January 2009.
Following its world show debut in Paris earlier this month, Renault has now confirmed pricing for its eagerly awaited New Laguna Coupe, ranging from 20,995 to 27,995.
The new Laguna flagship has more than just the sector-leading BMW 3-Series Coupe in its sights. With a raft of high-performance engines, impressive equipment levels and 4Control chassis in its Coupe GT guise, the svelte four- seater has a formidable arsenal.
In keeping with its elegant and muscular lines, the Coupe naturally receives the finest engines from the New Laguna range, as well as two new ones of its own, both with the advent of V6 power. At launch, four engines will be available, evenly split between petrol and diesel: 2.0 Turbo 205 and 3.5 V6 240, followed by the 2.0 dCi 150 and 2.0 dCi 180 FAP. The latter receives a 5hp power increase from the current dCi 175 in the rest of the New Laguna range, together with a particulate filter. The new flagship diesel engine, 3.0 V6 dCi 235 FAP, arrives in early 2009. Automatic transmission is available on the 2.0 dCi 150 and standard on 3.5 V6 240 and 3.0 V6 dCi 235.
As befits a luxury coupe, standard equipment levels are impressive. Coupe trim level includes Arkamys 3D Sound 140W audio system with MP3 CD reader and Bluetooth, 18" 'Interlagos' alloy wheels, eight airbags, automatic lights and wipers, dual zone climate control, electric heated door mirrors, MP3 player connection, rear parking sensors, Super Fold rear seat system, twin chrome exhausts (except 2.0 dCi 150 manual) and xenon headlights.
The top-of-the-range Coupe GT benefits from Renault's innovative 4Control chassis, first seen on GT versions of New Laguna Hatch and Sport Tourer.
Developed jointly by Renault and Renault Sport Technologies, 4Control, the four-wheel steering system, ensures the ultimate in driving pleasure, handling, dynamic response and steering precision.
The 4Control chassis brings many benefits, starting with improved maneuverability, sharpened dynamic response, and unrivalled steering precision giving the driver truly intuitive control over the cornering line, achieving new heights in drivability and active safety. In town and on country roads, the car is easy to drive: highly maneuverable and precise. The car also corners with very little body roll and a precise line is achieved through small steering inputs. Last but by no means least, the 4Control chassis excels in active safety, both under difficult braking conditions (with impressive control over asymmetrical grip and with the ESP matched to a more sporting driving style), and during avoidance maneuvers at speed ('elk' test). ??In addition to its 4Control chassis, flagship Coupe GT specification adds: 18" 'Celsium' alloy wheels, cruise control, automatic parking brake, electro chrome rear view mirror, electrically heated and folding door mirrors, GT leather steering wheel, leather upholstery (choice of beige, dark carbon, light grey or 'camel'), Renault Hands Free keycard and speed limiter.
At 22,345, the entry-level petrol, Coupe 2.0 Turbo 205 undercuts its equivalent BMW rival, the 325i SE Coupe, by a not insignificant 6,805 at list price, even without taking into account its additional 1,000 worth of equipment.
As well as its generous equipment list, the New Laguna Coupe range offers ample opportunity to tailor versions to individual tastes through several options:
700BOSE sound system (10 loudspeakers and amplifier)
400Front and rear parking sensors (Coupe GT)
300Heated front seats
500Heated front seats with electrically adjustable driver's seat with memory and folding door mirrors with memory
450Metallic paint
975Full color 2D satellite navigation with Bluetooth
1,750Full color 3D satellite navigation with Bluetooth and 230W audio system MP3 6CD changer (in-dash)
2,200Full color 3D satellite navigation with Bluetooth and 230W audio system MP3 6CD changer (in-dash) and BOSE sound system
400Twin chrome exhausts (Coupe dCi 150 manual)
250Tire pressure monitor


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LMFAO: Beyonce Makes A Fan Say Her Name Twice & Throws Her Towel On Him After Wiping Down Her Face
D reports "Beyonce in Chicago @ the United Center. She asks a fan to say her name (twice), starts singing to him, then gives him her towel after wiping down her face!! lol LOVE his reaction!! =) "
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Scholarship Support in Action - Shelby Solomon '13
Shelby Solomon is a sophomore at Saint Mary's and is taking advantage of opportunities both in and outside the classroom. This Honors Program student with a major in psychology enjoys connecting directly with her professors. She is currently vice president of the Psychology Club and will be president in her junior year. She joined the Boxing Club—something she never would have tried had Saint Mary's not encouraged her to branch out, try new things, and establish herself as a leader. Shelby also has volunteered on campus for Carnival 4 Kids and with a project to assemble water purification devices for third world countries. Thanks to the Class of 1950 Millennium Endowed Scholarship, she is pursuing her dream of becoming a forensic psychologist. "I am so thankful for this scholarship. I look forward to contributing to Saint Mary's in the future because I know how much it helps."


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Marathon runner in race to help ailing wife
The Boston Globe
By Bella English | GLOBE STAFF
Rick Kaitz had run three Boston Marathons and swore he'd never do another. "Training in winter in New England is awful," said Kaitz, 58, a Boston attorney. "It's so cold, icy, and dark."
But on April 15, he was at the starting line in Hopkinton, this time racing for his wife's life.
Initially told she had benign fibroid tumors, Erica Kaitz was diagnosed last year with uterine leiomyosarcoma, or LMS, a rare and aggressive cancer of the smooth muscle cells. She has an even rarer subtype that affects 50 to 100 people a year in the US. The couple consulted the top three cancer institutes on the East Coast: Dana-Farber, Duke, and Memorial Sloan-Kettering in New York.
"We had three different recommendations for treatment," he said. "It became clear that the top doctors don't know anything about it."
Because "orphan diseases" affect so few, there is little funding for research and few drugs manufactured. Kaitz knew he had to act fast. An athlete who has participated in many charity events, he figured his best chance of raising money would be through "Erica's Entourage," a team of friends who would run in the Boston Marathon for her, or ride in the Pan-Mass Challenge — or both.
He named it the Erica Kaitz LMS Research NOW Fund (www.ericasjourney.com) at the Dana-Farber Cancer Institute. All proceeds will go to research.
The night before the Boston Marathon, 19 supporters of Erica's Entourage attended a team dinner at Trade on Atlantic Avenue. Spirits were high.
On Marathon Monday, it was 38 degrees at 6:45 a.m. when Kaitz left the couple's condominium at the InterContinental Boston. At 10:45 a.m., he and his teammates — law partner Bethany Bartlett and Tim Simonson, who is married to Erica's cousin — took off in the last wave at Hopkinton, with the other charity runners.
Kaitz paced himself. He'd told his wife that he'd be at mile 20 between 2 and 2:30 p.m, and he was. Erica was waiting with a hug, a Gatorade, and a caffeine gel for her husband."He's amazing," she said. "He can tell me exactly where he's going to be in a race, and when, and he'll be there."
The same can be said of their marriage. They met in 1986 on a blind date. "We were engaged in six weeks and married in a couple of months," said Erica, 52. Their daughters are 21 and 23.
The couple has taken a team approach to her cancer, which they discussed on a recent day as Erica was undergoing chemotherapy at Dana-Farber.
"I focus on my treatment and I also do a tremendous amount of integrated therapies, such as Qui Gong, acupuncture, and meditation," she said. "Rick focuses on the LMS part of it, figuring what's out there in terms of meds, reading the literature, keeping in touch with the LMS community, to push our project ahead."
Indeed, Rick Kaitz uses the word "we" when he speaks of her cancer: "We elected to have the second surgery right after the Pan-Mass Challenge last summer." He has gone to all of her appointments, and considers himself lucky to have a flexible workplace at the firm of Sherin and Lodgen that allowed him a medical leave; now he works part-time around her schedule.
Billy Starr, executive director of the Pan-Mass Challenge, went to junior high and high school in Newton with Rick, and Erica has ridden, volunteered, and, since 2000, worked for the Pan-Mass Challenge.
Starr is impressed with the couple's perseverance under fire. "Ricky hasn't lost his focus, his purpose, even his sense of humor," he said, noting that Erica's Entourage has already raised $95,000 for the bike-a-thon, which isn't until August.
At the Marathon, after he and Erica hugged, Kaitz was feeling sore all over. But he knew the worst was behind him. "The last mile is actually fun, because you know it's over," he said.
Only this time, it was neither fun nor over. At 25.7 miles, half a mile from the finish line, Kaitz began to notice "weird stuff." First, there were the ambulances going up Beacon Street.
"It wasn't hot out, and I wondered what that was all about," he said. "The next thing that was definitely very weird was, after Kenmore Square, there were medical volunteers in white jackets handing out Mylar blankets. Usually, they come at the end, at the chutes. You get the water bottle, you get the blanket, and then the medal, that's how it works."
Then he saw other runners walking the wrong way on the course. Finally, the pack came to a standstill. He heard the chatter: an explosion at the finish. "My first reaction was that it must be a manhole explosion," said Kaitz. "The thought of a terrorist attack at the Boston Marathon was not high on my radar screen."
It took Kaitz what seemed like forever to make it back to the Boston Common Hotel on Trinity Place, where he and his teammates had booked a room for a shower and a quick massage.
Meanwhile, Erica had arrived home and was in the lobby of her building talking about what a great day it was when someone mentioned the explosion. She was worried: "I needed to hear Rick's voice." But not until 5 p.m., when he arrived at the hotel, was he able to reach her.
"I felt very fortunate to wake up Tuesday morning with him beside me," she said.
When her chemotherapy is over this summer, she may have experimental treatments to keep the tumors at bay while researchers race to find more options.
"It's an incurable disease, and we are trying to do everything we can to give benefits not just to Erica, but to others with orphan diseases," said Dr. George Demetri, director of the Center for Sarcoma and Bone Oncology at Dana-Farber. His lab is one of the few in the world working on LMS.
Rick Kaitz knows that the money he's raising is small compared to the multi-billion-dollar "war on cancer," but medical researchers often labor in small laboratories supported by small grants. Kaitz has raised about $200,000 from the Marathon and a half-marathon he did in October, but the Pan-Mass Challenge will be his biggest effort.
"We have 80 people signed up for our team, and about a half dozen other teams supporting Erica's fund," he said. "We should raise a million and a half from the PMC."
The irony that they now need the PMC and Dana-Farber isn't lost on the Kaitzes, who between them have raised $400,000 over the years for the effort. Rick has ridden 20 Pan-Mass Challenges, Erica half a dozen. Last year, their daughter Alyssa rode, and she will ride with her father again this year.
Erica has worked for the PMC most recently as the event planner. In fact, she planned this year's Heavy Hitter Dinner, held the Friday before the Marathon, which honors the biggest fund-raisers for the two-day cycling event. The dinner always features a patient talking about how the PMC has helped. In previous years, she'd helped line them up.
This year, she was the speaker. She spoke about how she had been coming to the Dana-Farber for years, first as a caregiver for her mother who had cancer, and later as a donor and volunteer.
"This year," she said, dabbing away tears, "I'll be donning a new hat. It will be my proudest PMC hat yet, that of a survivor."
She got a standing ovation from the audience and a hug from her husband.


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Submitted by TimothyMorton on
In Ecology without Nature I argued that it was the very idea of Nature itself that posed an obstacle to ecological thinking and praxis. Nature has recently received various upgrades, for example in the form of ecophenomenology, which insists that we are embedded (like Iraq War reporters) in a lifeworld. This language comes from Heidegger.
If the ecological thought is to tunnel back behind Heidegger, it must encounter the thinking of Emmanuel Lévinas.
Lévinas argues that below Being, beyond essence, closer than breathing, is the proximity of the really other other, the strange stranger in the language of The Ecological Thought. Unnameable as such, yet still palpable as an excess or "lapse" within the ontological order of things, is "the face," Lévinas's word for the strange stranger as we encounter her, or him, or it. Our very being is both subtended and interrupted by the face of the other, who stands destitute before us and before whom we too are destitute. Sadder and wiser.
In Otherwise than Being, Lévinas declares that the face is "the collapse of phenomenality," in that it is "too weak" and "less than a phenomenon" (88). The face of the neighbor causes "the human" to "shudder" (87). The asymmetrical contact between us and the face causes us to revert "from grasping to being grasped, like in the ambiguity of a kiss" (80). More passive than inertia, this contact is "an inversion of the conatus [Spinoza's word for the will to live] of esse" (75). It is "not an extrapolation of the finite, or the invisible taken to be behind the visible" (154). It is on this side, "a hither side of the here" (180). This would be an encounter that did not take place within a lifeworld. A non-holistic reality where my obligation subverts the creation of worlds, with their insides and outsides, their included and excluded. Where identity was not—where personhood and phenomenality were less than you expected.
Is this not the shuddering of the Wedding Guest before the Mariner?
But is it not also the shuddering of the Mariner before Life-in-Death, a face consumed with leprosy?
And might it contain an encounter with the sheer existence existing things—"a thousand thousand slimy things" as the Mariner says (4.238)? This existence, which includes the Mariner's own "liv[ing] on" (4.238). Might this ecological "element" in which we are immersed also be visible in the disturbed and disturbing face of the other? And is the ecological thought therefore a coexistence with coexistence itself (and with coexistents, as it were)? Lévinas grants this possibility when he asserts that the "absurd" there is (his phrase for sheer existence) can be "a modality of being-for-the-other" (164). In fact, you need the "weight" of this existence for alterity to go beyond essence (164). Lévinas himself opens the possibility that the there is with its incessant "splashing" (140) might be the face itself, as if the face were glimpsed obliquely, that nothing truly separates background from foreground, just a "strange distortion" (Shelley).
Something like this happens in The Triumph of Life, when in an Arcimboldo moment, a root twists into the face of Rousseau. And it happens in Frankenstein, when a creature made of pieces of animals and human corpses rises up to embrace its terrified maker.
Isn't this the horror of Life-in-Death? The gentleness and ambiguity of her threat to essence? Her existence is ambiguity in all its fullness. She does nothing, really. She merely appears; she casts dice; she speaks, and whistles. Her face rots away. She floats on the sea, space of nomads, pirates, traders, gamblers, not tied to the State, beyond the law. She is as passive as the Mariner. It is finally her passivity that disturbs. We behold her; we are beholden to her.


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Spring Zing!
Product DescriptionSend Flowers! Brighten A Room! Lift Their Spirits! Show You Care! with Flowers from DELLA'S MAPLE LANE FLORIST. This Spring Zing! bouquet is full of Springtime joy and sweetness with a mix of carnations, tulips, pom-poms and other fresh flowers. Call to send flowers or order an arrangement from our website 24 hours a day!
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Lost cat - mountainside/brant area - burlington, on -please help
Hi All,
Sadly, my beloved cat Barney managed to sneak out of our house unnoticed the evening of Jan 30th. He is an indoor cat and has not been outside since before we had adopted him almost five years ago (he was a stray). He is a beautiful grey/black/brown tabby with green eyes, and a small tick missing from his left ear. Please see attached photos. He is neutered, not de-clawed and very friendly. I am beside myself with worry as weather has been very cold/snowy since he escaped.
If you think you may have found him, please let me know as we are hoping for his safe return as soon as possible.
Thanks so much.
If you can put his litterbox and/or a worn but unwashed t shirt or pair of smelly shoes of yours out on the deck this may help lead him home
Go around the neighbourhood at dusk/dawn calling him, rattling a treat container or tap a spoon on his dish or tin of food. Bring a flashlight to pick up the shine off his eyes from under cars/decks/bushes or up trees.
If you can poster the neighbourhood, plus leave one w/ local businesses, schools, pet supply stores, grocery stores, bus stops, vets & all animal shelters in the area that will help garner attention that this kitty has a home who wants him back
Please visit the animal shelters in person often times the shelters are understaffed/over worked with so many animals/requests etc they may not have the time to personally look into each phone call or an emergency/something comes up in the meantime & the request gets overlooked.
Going in person means you can visit & identify the cat right away, if an animal comes in terrified it may act out of character & be labeled as sick or a feral and not be placed with the general population please ask to see these areas as well.
Good luck &
Avoid biting when a simple growl will do
The Spirit Lives As Long As Someone Who Lives Remembers You - Navaho Saying
Vindication ~ For all those pets who became sick or lost their lives from tainted pet food
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Nurse Supervisor Jobs >> Browse Articles >> Work-Life Balance
8 Things You Should Never Say to a Patient
Nina Kim, Georgia Price | NursingLink
August 05, 2009
5. "We're very busy. Please wait."
Trying to brush off a patient's aggravation by saying you're busy only makes things worse. Apologize and make your best attempt to smooth things over once the patient is able to be seen.


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Carmen Ortega Says She Caused Kim Kardashian/Reggie Bush Break-Up & Has The s3x Tape To Prove It [Audio]
Carmen Ortega Says She Has a Reggie Bush Sex Tape
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0 Members and 2 Guests are viewing this topic.
By the way, which card lineup did the Locus image come from?
they'd fit nicely as avatars if Walter was ok to include them in the database (there's no way I'll host them).
One day, when I'm not too lazy. Maybe today? But probably not...
I tried to do a good old color the other day... I can't even stay in the lines anymore. =)
No one will see my shame.
Me either. THat vritannis image I did yesterday looks like I colored it while on PCP. Thankfully I was forced to shrink it down to 200 pixels. No one will see my shame.
What this morning's attempt yielded, tried to keep it simple, stylish and cinematic. Take your pick; dusk or sunset Guts. =)
I like the left one better. Couldn't say if it's dusk or sunset though .
I got some avatars for some of you folks. If you need em bigger or smaller (though I doubt you'll want em smaller) just say the word.
Isn't Casca supposed to have black eyes?
Isn't Femto blue!?
nooo, he's yellow, you idiot
Are you trying to be funny or something?I thought Casca's eyes were supposed to be black and that was in a touching part of the story I' ld say.
Mind if I use that grunbeld avatar for a while?


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No one wants to work with Scott Weiland!
Scott Weiland is now weighing in with his own official response to today's news that he has been fired from Stone Temple Pilots. The vocalist stated as recently as yesterday that all was still good with his longtime band, telling Rolling Stone, "STP has not broken up. I haven't quit. I haven't been fired." He even added that they were talking about when they wanted to tour again.
However, this morning, a press release arrived from STP with the one-line statement reading, "Stone Temple Pilots have announced they have officially terminated Scott Weiland." Rumors of discord had been rampant in the band since Slash revealed in an interview earlier this year that part of the reason for Weiland's public overtures about returning to Velvet Revolver had to do with his being ousted from STP.
With the news breaking about his termination this morning, Weiland has responded with the following statement:
I learned of my supposed "termination" from Stone Temple Pilots this morning by reading about it in the press. Not sure how I can be "terminated" from a band that I founded, fronted and co-wrote many of its biggest hits, but that's something for the lawyers to figure out. In the meantime, I'm looking forward to seeing all of my fans on my solo tour which starts this Friday. – Scott Weiland
Speaking of that 'Purple at the Core' solo tour, it's been revealed that Weiland intends to focus primarily on music from the 'Purple' and 'Core' albums with his solo band, though there is expected to be some songs from other albums and even his Velvet Revolver career sprinkled in.


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Country music star Tim McGraw collapsed on stage during a performance in Ireland.
McGraw collapsed while performing Sunday night in Dublin.
McGraw's wife, singer Faith Hill, can be seen in a video on a fan's Instagram page saying, "He's been super dehydrated. I apologize, but I made the decision that he cannot come back out on stage."
READ MORE: Heather Locklear told police 'I will shoot you,' and other arrest details
A representative for McGraw issued a statement saying McGraw was attended to by local medical staff on-site and will be fine.
McGraw, who was performing as part of the Country to Country festival in the U.K., had performed Friday night in London and Saturday night in Glasgow as part of C2C, a three-day, three-city country-music festival.
The duo is scheduled to begin their 29-city Soul2Soul tour in the U.S. on May 31 in Richmond, Virginia.
(You can watch the video, top.)
© 2018 The Canadian Press


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Phil Stott
March 18, 2014
As a follow-up to my recent post on the lessons I learned from the best jobs I've had, I've been thinking about some of the worst—or least enjoyable—positions that I've held in my two-plus decades in the workforce. While there have certainly been plenty of periods in my career where I haven't enjoyed the work I've done, or taken much more from it than the paycheck at the end of the day, there are a handful of positions that stand out as having been particularly dispiriting and difficult to put up with.
Bearing in mind the old saw that "if it was fun, it wouldn't be called work", what is it that makes a position unbearable? In my case, so far, it's been a number of different factors. First: finding work that I'm just not suited for, and where there are no opportunities for learning or advancement. Second: no likeable colleagues—a factor that has worked as a redeeming feature in jobs that I otherwise would have given up on sooner. And, finally, the worst job I ever had was for a company that I felt was acting immorally, if not illegally, and definitely without having its customers' best interests at heart. Being made complicit in that, even for a short while, ranks as the single worst career experience I can imagine.
Here, then, are the worst jobs I've held to date, and some of the lessons I've taken from them:
3. Gas station attendant
Your first job is supposed to be a completely lousy experience, right? Mine certainly was: 4-hour shifts in a gas station that took me an hour to get to. Apart from the mind-numbing tedium of minding the register during the slow evening period, duties included keeping the rarely perused shelves and fridges stocked and…not much else. The obvious solution to a complete lack of anything to do—my high school homework—was expressly forbidden by the owner, who would show up at random times to check on the place and offer occasional wisdom on how to deal with eventualities like shoplifters ("prevent them from leaving, if you can") and stick-ups ("give them the money in the register, but don't mention the safe"). He would then disappear again until closing time, leaving me, at 15, in sole charge and convinced that I was in imminent danger of having to put that advice into practice.
The worst part: While work-life balance isn't a term that teenagers throw around too often, I got an early induction into one of the realities of the working world—that you can't do everything you want and everything you need to. So as soon as I'd banked enough money to buy the sneakers that had prompted my job search in the first place, I quit to free up my evenings and weekends for pursuing my true passion: Championship Manager.
2. Fast food worker
Don't get me wrong here: I'm not denigrating the entire world of fast food employees. Just the specific job that I held, with a British burger chain that was stationed within the same bowling alley—and had the same managers—that I listed as one of my favorite jobs. So what was so different about it? For one: the smell. No amount of laundering could get my uniform fresh. For another: isolation. I was often the only employee manning the place, meaning that I did everything from taking the orders and handling the cash to cooking the food, and cleaning the restaurant at close. Without the experience to realize that asking for help was not a sign of weakness, it often became overwhelming.
The worst part: The state of my hands. When you have to alternate register duties with spells at the griddle, you're going to wind up washing your hands a LOT (at least if you care about hygiene—some of my colleagues were less punctilious). Add in the regular burns from said griddle and the fryers, and it was sometimes tough to tell where the hamburger meat ended and my flesh began.
1. Credit card call center employee
Hands-down my worst career experience to date is the period I spent working for a major British bank, as a customer service representative in its credit card division during the height of the credit bubble. In retrospect, many of the signs that led to the end of the bubble were in evidence every day: people who couldn't pay their existing bills being offered ever more credit; bonuses dependent on the quantity of loan leads we generated; dealing with customers who clearly hadn't understood—or had explained to them—the terms of their contracts. Not to mention the call center staples, like being verbally abused over anything from a forgotten password or account servicing fees, all the way up to threats of legal action on a delinquent account.
As employees, meanwhile, we were treated as little more than numbers: software tracked and recorded every call and click, and call volume and efficiency were included in our monthly targets—a couple of complex (i.e. long) calls could mean the difference between taking a bathroom break or being able to meet your quota.
The worst part: Working somewhere that I knew was—at best—relying on its customers to not read the fine print when signing deals (indeed, subsequent to the financial collapse, UK law changed to outlaw some of the practices I had seen while working there). Worse than that, though, was that I dealt with so many complaints about the practices that I became immune to the outrage, and came to view them as a hindrance (angry customers make for longer calls; longer calls make for missed bonus targets), instead of treating them as genuine—and valid—complaints.
Have your say: What are the worst employment experiences you've had, and why? Let us know in the comments below, on Facebook, or on Twitter.
Related: Great Jobs: What I've Learned from the Best Jobs I've Held
Great Jobs: What I've Learned from the Best Jobs I've Held
March 12, 2014
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Job Search
Workplace Issues
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text:
Using WhatsApp marketing we can easily send
massive amount of What'sApp messages to your targeted
customers As we all know WhatsApp is really a great
tool for messaging and is nearly free of
cost. It has got everything including SMS, MMS. But today
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and have decided to go into WhatsApp marketing
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You can send large amount of messages without
any limit. And it is available for all smartphone
platforms including Nokia, Blackberry, iPhone, Android , Windows
Mobile, Symbian. Every one is using it because you don't have to
go to a website then fill details and then confirm our identity
to use it. It verifies your mobile number and you
are done! It's more than just an SMS. You can easily
send your latest product images or company coupons
to your customers without paying the expensive
MMS costing. You can also send the GPS locations
of your defined stores! Audio, Movies & Emoji icons are also supported.
Hence WhatsApp can perform all those thing which
you are not able to do with SMS services and Emails
V-Touch Solutions provides a new channel for you to reach your customers using WhatsApp. You can broadcast your coupon images, promotion messages, emoji icon, shop locations or even some movies directly to your customer's smartphone. A very new efficient way of mobile marketing. V-Touch Solutions Whatsapp message services supports the different languages like Punjabi, Gujarati, English, Hindi, Bengali…etc. Don't hesitate, try out our WhatsApp marketing service and contact us at +91-9305738173 for more details.
Organisations that are use our SMS services include
Here are the reasons why you should choose us for all your search engine marketing needs :
Whatsapp Marketing is one of the platform for any businessman or owner to promote his product. You will get direct access to the various customers. The mostly used messaging cross platform application by the people since it has been launch. So today are going to have a look on Whatsapp strategies of Marketing, so that the small business owners can take the opportunity. V-Touch Solutions guarantees you, for cracking more sale once you start using Whatsapp Marketing for your business promotion. Gone are the days of email and of the SMS marketing, today what we are talking about is the reliable and fastest promotional weapon for your business named Whatsapp. Everyone is aware of TRAI rules, that anyone can not send messages to any of the DND numbers. So it means SMS marketing is a waste now. If we talk about Email marketing then Well how many of the advertising emails you have reply since you started using your first email account. This is what exactly am talking about. Email marketing is basically waste if you haven not done your homework right. So what we actually do is, set up a campaign to promote your business to the list of number, either provided by you or by us (It is totally depends 0n you). Now we got a bot which is attached to Whatsapp's official version. V-Touch Solutions then be loading your promotional message and in a few minutes that message will be hitting everyone's Whatsapp applications. The Prices are really low, Writers, Small Businesses, and Many IT Company, Hotels have used our Whatsapp Marketing Service. They are getting new clients every day and their revenue is been increasing day by day.
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Taylor Swift celebrated leaving her teen years behind with a holiday-themed party for her friends, band and tour crew – but the biggest gift of the night came from the singer herself.
According to People, the singer marked turning 20 by giving $250,000 to schools around the country that she's attended and worked with over the years.
VIEW THE PHOTOS: Are They Or Aren't They? Hollywood's Hottest Rumored Couples
"[It's] something I wanted to do at the end of this amazing year and especially on my birthday was give back to something I really believe in, which is education," Taylor told the mag. "The schools that I went to and the amazing people I got to learn from really turned me into who I am, and I wanted to give back."
The donated money will reportedly buy books for students, pay teacher's salaries and fund numerous educational programs.
VIEW THE PHOTOS: Taylor Swift: From 'Fifteen' To Country Queen!
As for her holiday-themed birthday party that Taylor baked dozens of butter and gingerbread cookies for, the singer said she's happy to combine the holiday spirit with her special day.
"It's more like an end-of-year Christmas party that happens to be on my birthday," she added.
No word if her good friend Taylor Lautner was in attendance.
Related Content from AccessHollywood.com:
VIEW THE PHOTOS: 'Twilight' Heartthrob: Taylor Lautner
PLAY IT NOW: CMA Awards 2009: Taylor Swift - 'It Felt Like A Dream'
PLAY IT NOW: Access Flashback With Taylor Swift
MORE ACCESS ON THESE TOPICS: Taylor Swift - Taylor Lautner - Christmas - Young Hollywood - TV


text: This news item expired on 9/21/2013, so the information below could be outdated or incorrect. Art & Coffee Table Book Sale - September 21 The Friends of Weaverville Library will have a special sale of art books and coffee table books on Saturday, September 21 from 10 a.m. - 3 p.m. in conjunction with the town's annual "Art in Autumn" juried art show. We've been saving the best of the best for this sale, so don't miss it! All proceeds will benefit the Weaverville Library. For more information, call 250-6482 or email email@example.com.


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Student or Learner
The gap is increasing.
The guy is running.
The baby is sleeping.
No one calls increasing and running and sleeping adjectives, but how increasingly can be an adverb and it is possible? but I have never seen runningly, sleepingly, etc. Is there a particular reason for the word, increasingly? Thank you so much as usual and have a good day.
Thank you and you agree that increasing in the sentence functions as a verb, not an adjective, right? Thank you so much.
An increasing number of people prefer Facebook to Twitter.
is a participle which, in common with all participles, is inherently capable of serving an adjectival function (here modifying 'number').
However, that alone is not necessarily sufficient for it to be classed as a true adjective by the lights of most modern grammarians.


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9:00 pm
Moyers & Company "Fiscal Cliffs and Fiscal Realities" When it comes to America's economic health, all anyone seems to talk about is the "fiscal cliff," and the perils of our inevitable plunge. But media's favorite metaphor is distracting us from actual and crucial fiscal realities. On the next Moyers & Company (check local listings), independent political and economic analysts Bruce Bartlett and Yves Smith join Bill in a discussion that's become as rare as it is necessary -- why are Washington insiders talking about the deficit crisis and not the jobs crisis? Bartlett, former advisor to Presidents Ronald Reagan and George H. W. Bush, got into hot water with fellow conservatives when he aired concerns about the direction of their ideology and wrote critically of the second George Bush. D
10:00 pm
Linkasia NULL
10:30 pm
11:00 pm
McLaughlin Group NULL
11:30 pm
Need to Know RAY SUAREZ ANCHORS. Correspondent Stacey Tisdale travels to Memphis to examine if the city's experimental "Family Rewards" program is helping combat poverty. The program pays low-income students and their parents for everything from good attendance and good grades to regular visits to doctors and dentists. D


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Is a grand bargain only way out?
Many pragmatic House Republicans have come to a simple conclusion: Navigating their way out of this fiscal mess won't be easy.
They think their best chance to fund the government, raise the debt ceiling and extract any concession from President Barack Obama is to strike a big budget deal.
Story Continued Below
Yes, the grand bargain is back.
It's about the only common thread these days that runs from House Republican leadership down through the rank and file. Most House Republicans privately concede they're fighting a battle they're unlikely to win, and to avoid a prolonged shutdown and a disastrous debt default, Washington has to create a package so big that lifting the borrowing limit and funding the government is merely a sideshow.
( POLITICO's full government shutdown coverage)
The players are well-positioned, the current situation is seemingly intractable and both parties want to slice the debt. But the prospect of a grand bargain faces both immediate and longer-term hurdles.
President Barack Obama and Democratic congressional leaders refuse to negotiate until the government is reopened, and even then, they remain deeply skeptical that a sweeping deal is possible, given the GOP's opposition to new revenue.
When House Speaker John Boehner raised the idea at a White House meeting Wednesday with Obama and congressional leaders, "everybody laughed at him because they've heard this song and dance so many times before," said a Democratic aide briefed on the meeting. A Republican aide disputed the account, saying Democrats harangued Boehner to appoint budget negotiators. The speaker retorted, saying Senate Majority Leader Harry Reid (D-Nev.) will not allow Senate Budget Chairman Patty Murray (D-Wash.) to hold talks with House Budget Chair Paul Ryan (R-Wis.).
( POLITICO interview: Rep. Steve King predicts longer shutdown)
But it is gaining traction among Republicans.
"I want to get a budget agreement," Ryan told POLITICO on Wednesday. "That's what we've been about all along. So yeah, we think the issues are converging — [the continuing resolution] and debt limit — and from the get-go, we wanted to get a budget agreement to grow this economy and get this debt under control, especially before the Federal Reserve starts raising interest rates. I think it's in our nation's interest to do that. And that's one of the things we're fighting for in addition to relief from Obamacare — or fairness from Obamacare."
Ryan has discussed his goals on the debt ceiling at a Ways and Means meeting of Republicans. One senior Republican leadership source said this strategy, which has been bantered about at the top levels for some time, would be the "way out of the cul-de-sac."
( WATCH: Veterans storm World War II Memorial)
Boehner, who has longed for a massive budget agreement, has spoken about it with members in recent days, according to sources. And an aide described him as "aggressively open-minded" about how to solve the CR and debt ceiling. Boehner and Ryan have both publicly and privately talked about using sequester spending levels as a negotiating tool to entice Obama to negotiate.
The collision of a number of factors has sparked renewed Republican talk of a grand bargain.
Privately, a healthy number of pragmatic Republicans understand that Obamacare is not going to be defunded — or even touched — in the debt ceiling and government-funding negotiations. At this point, the strong stand against funding government is more about standing firm against Democrats than actually stripping money from Obamacare.
Obama isn't running for office again, and few Republicans think Boehner will remain in the top post for much longer. Ways and Means Chairman Dave Camp (R-Mich.) — who has longed for a rewrite of the Tax Code — is nearing the end of his chairmanship. Ryan — who some see as the speaker-in-waiting — is desperate for a budget agreement.
So with the government shut down and the debt ceiling rapidly approaching in the next two weeks, a large number of Republicans see a grand deficit compromise as the only plausible way out.
Members throughout the House Republican Conference are even sounding a new tune on a potential deal. There are fresh signs Republicans would consider new revenue if they are not raising tax rates, and key Republicans told POLITICO that they would be interested in some of the items discussed by Boehner and Obama in 2011.
Republican Rep. Steve Stivers, a close Boehner ally from the Columbus, Ohio, area, said "guys like me" would consider revenue in a potential deal.
"We're getting closer to the point that everything's intertwined — government funding, the debt ceiling and spending — and so the best way out, I believe, and the only way for everybody to find an acceptable long-term solution is a big negotiation of everything that includes something on entitlements, tax reform, something on a spending level and wrap it in one box," Stivers told POLITICO.
Obama, too, has long argued that a grand bargain on the debt and deficit is the only way to move beyond the cycle of fiscal crises on his watch. He reiterated Wednesday that he's open to pursuing a big budget deal — but not until the government shutdown is over and the debt limit is raised.
"The message I have for the leaders is very simple," Obama said in a CNBC interview. "As soon as we get a clean piece of legislation that reopens the government, and there is a majority for that right now in the House of Representatives. Until we get that done, until we make sure Congress allows treasury to pay for things that Congress itself already authorized, we are not going to engage in a series of negotiations."


text:
yiseowl needed a new other design and created a contest on 99designs.
A winner was selected from 105 designs submitted by 17 freelance designers.
Mayan Minds
Mayan Minds is a t-shirt brand dedicated to bringing fresh and creative designs to today' s urban alternative crowd. Inspired by the ancient Mayan civilization in South America, Mayan Minds is looking to blend ancient symbols and glyphs with modern fashion and design. We want to mix the old with the new to bring a dynamic and artistic appeal look to the marketplace.
Our target audience is the alternative hipster crowd of teenagers and young adults who has an affinity for the urban hip hop and urban alternative flair. People ages 15-30 who have a knack for original and creative apparel are the foundation of Mayan Minds.
We would like for each designer to propose 1-3 t-shirt designs. While we have certain glyphs in mind, we're also not opposed to you choosing your own.
Our only criteria is that you use a Mayan glyph(s) on each shirt, customize the design for a short or long sleeve t-shirt, and go creatively beyond just taking a Mayan glyph off the internet and sticking it on a t-shirt. Bring originally to the Glyphs. Feel free to deconstruct them, erode them, stylize, and/or vectorize them. For inspiration, here are 4 t-shirt designers/designs we like:
1. http://www.johnnycupcakes.com/blog/
2. http://www.aqua-vi.com/
3. http://tinyurl.com/kob8w6
4. http://tinyurl.com/l42gn3
-Also, here are some great vectorized Mayan fonts for free: (http://www.dafont.com/font.php?file=mayan)
-And this site indexes pretty much every glyph: (http://www.famsi.org/mayawriting/di…nindex.htm)
Our ideas and favorite glyphs:
1. Mayan footprint: (http://tinyurl.com/lh6o6r). Use small footprints on the top of the backside of the shirt that progressively get bigger to the front bottom.
2. Face in hand: (http://tinyurl.com/mecefo)
3. An inverted sky: (http://tinyurl.com/neou64)
4. Wings: (http://tinyurl.com/l65qhq)
5. Outstretched human hand: (http://tinyurl.com/mpd4kh) & (http://tinyurl.com/mfou73)
6. Boomerang: (http://tinyurl.com/md7xut)
7. Traditional face: (http://tinyurl.com/lfss45)


text:
Ashlee Simpson splits from Vincent Piazza
Singer ASHLEE SIMPSON has reportedly called time on her romance with VINCENT PIAZZA.
The pop star split from the Boardwalk Empire actor over the Thanksgiving holiday (22Nov12), according to New York Post gossip column Page Six.
A source tells the publication, "Vincent has been working a lot, so he hasn't been able to spend as much time with Ashlee, which has caused some problems. It was a mutual agreement to split, but things have been bumpy for a little while, so perhaps they might reconcile."
Simpson began dating Piazza in June 2011 after ending her marriage to rocker Pete Wentz, the father of her son Bronx Mowgli.