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Science. Vol. 267 24 March 1995
Click Here to See Cover Photo
Georg Halder,* Patrick Callaerts,* Walter J. Gehring^
Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland.
*The first two authors contributed equally
to this work.
^ To whom correspondence should be addressed.
The Drosophila gene eyeless (ey ) encodes a transcription factor with both a paired domain and a homeodomain. It is homologous to the mouse Small eye (Pax-6 ) gene and to the Aniridia gene in humans. These genes share extensive sequence identity, the position of three intron splice sites is conserved, and these genes are expressed similarly in the developing nervous system and in the eye during morphogenesis. Loss-of-function mutations in both the insect and in the mammalian genes have been shown to lead to a reduction or absence of eye structures, which suggests that ey functions in eye morphogenesis. By targeted expression of the ey complementary DNA in various imaginal disc primordia of Drosophila, ectopic eye structures were induced on the wings, the legs, and on the antennae. The ectopic eyes appeared morphologically normal and consisted of groups of fully differentiated ommatidia with a complete set of photoreceptor cells. These results support the proposition that ey is the master control gene for eye morphogenesis. Because homologous genes are present in vertebrates, ascidians, insects, cephalopods, and nemerteans, ey may function as a master control gene throughout the metazoa.
The eyeless (ey) mutation of Drosophila was first described in 1915 (1) on the basis of its characteristic phenotype, the partial or complete absence of the compound eyes. The ey alleles available today are recessive hypomorphs (weak alleles) and they lead to the reduction or complete absence of the compound eyes but do not affect the ocelli (simple eyes) on the head of the fly. Apparent null alleles that are lethal when homozygous have also been isolated (2), but they have been lost, and a detailed analysis of their phenotype is not available. Cloning and sequencing of the ey gene (3) have shown that it encodes a transcription factor that contains both a paired domain and a homeodomain. The ey gene is homologous to Small eye (Sey = Pax-6 ) in the mouse and to Aniridia in humans. The proteins encoded by these genes share 94 percent sequence identity in the paired domain, and 90 percent identity in the homeodomain and they contain additional similarities in the flanking sequences. Furthermore, two out of three splice sites in the paired box and one out of two splice sites in the homeobox are conserved between the Drosophila and the mammalian genes, which indicates that these genes are orthologous.
Both the mouse and the Drosophila gene have similar expression patterns during development. In the mouse, the expression of Sey is observed in the spinal cord, in discrete regions of the brain, and in the developing eye. The Sey gene is expressed from the earliest stages until the end of eye morphogenesis: first, in the optic sulcus, and subsequently in the eye vesicle, in the lens, in the differentiating retina, and finally in the cornea (4). In Drosophila, ey is first expressed in the embryonic ventral nerve cord and in defined regions of the brain. Later in embryogenesis, ey is transcribed in the embryonic primordia of the eye as soon as these cells can be detected. In subsequent larval stages, it continues to be expressed in the developing eye imaginal discs. During the third larval stage, ey expression becomes largely restricted to the part of the eye disc that is anterior to the morphogenetic furrow. This region consists of undifferentiated cells whereas posterior to the furrow the differentiating ommatidia are apparent (5). Because mutations in the mouse and Drosophila genes lead to a reduction or complete absence of all eye structures, and because these genes are similar in DNA sequence and in expression pattern even at the earliest stage of eye development, it has been suggested that ey and Sey may be the master control genes involved in eye morphogenesis (3). Furthermore, mutations in four other Drosophila genes with similar phenotypes (eyes absent , sine oculis , eye gone , and eyelisch ) do not affect the expression pattern of ey, which indicates that ey acts upstream of these other genes (6). These results are consistent with its possible role as a gene that controls eye morphogenesis, even though it may have additional functions in the developing nervous system. The cloning of the homologous genes from ascidians, cephalopods, and nemerteans (ribbon worms) suggests that this gene may be present in all metazoa (3).
Master control genes that act as developmental switches can be detected on the basis of their mutant phenotypes. Thus, homeotic mutations have identified master control genes that specify the body plan along the antero-posterior axis. These genes, which are characterized by a homeobox, are clustered in the Antennapedia (Antp ) and Bithorax Complexes in Drosophila, and in the Hox gene clusters of the mouse (7). Loss- and gain-of-function mutations in these genes lead to opposite homeotic transformations. For example, in Antp , recessive loss-of-function mutations are lethal at the embryonic or larval stage and lead to a transformation of the second thoracic segment (T2) toward the first thoracic segment (T2 --> T1). Dominant gain-of-function mutations lead to a transformation in the opposite direction, that is from the anterior head and T1 segments toward T2 (H,T1 --> T2) (8). These transformations can be explained by the combinatorial interaction of several homeotic genes in order to specify a given body segment. These genes have partially overlapping expression domains in several body segments and each segment is specified by a combination of homeobox genes, that is by a Hox code (9). By ubiquitous (ectopic) expression of Antp under the control of a heatshock promoter, we have changed the body plan of Drosophila and induced the formation of middle legs in place of the antennae, and also transformed the dorsal head capsule into structures of the second thoracic segment (H --> T2). This phenotype is similar to that observed in dominant gain-of-function mutations (10). However, it proved to be difficult to transform the more posterior body segments toward T2. Data for several homeotic genes indicate that there is competition between the ectopically expressed gene and the genes normally expressed in a given segment (11). This competition frequently leads to epistasis of the posterior over the anterior genes, and to segmental transformations that are confined to the anterior body segments.
The ey gene, which also contains a homeobox in addition to a paired box, differs from Antp and the other antero-posterior homeotic genes in that the hypomorphic loss-of-function mutation leads to a loss of the corresponding eye structures rather than to their homeotic transformation. This phenotype does not necessarily imply that ey acts as a developmental switch; it only shows that ey function is required for eye development. If, however, ey is the master control gene for eye morphogenesis, the ectopic expression of ey should induce the formation of ectopic eye structures in other parts of the body similar to the transformations obtained for Antp (10) and the other homeotic genes (11). Therefore we used the GAL4 system (12) and a heat-inducible expression vector in order to express the ey gene ectopically.
Fig. 1. Targeted expression
of ey.
(A) Schematic representation of the ectopic induction of ey by means of the GAL4
system. In (B) through (D), ß-galactosidase staining of third instar
imaginal discs (28) shows the activation of a UAS-LacZ reporter construct by the GAL4 enhancer-trap
line E132.
(B) Eye-antenna disc. The antenna portion of the disc is on the top and the eye portion is on the bottom. ß-Galactosidase activity is detected in parts of the antenna disc corresponding to several antenna segments and in the periphery of the disc, which will give rise to head cuticle. The staining observed at the most posterior part of the eye disc derives from the optic nerve.
(C) Wing imaginal disc. ß-Galactosidase activity is detected in proximal regions of the future wing blade, and in portions corresponding to the hinge regions and ventral pleura.
(D) Leg imaginal disc with LacZ expression in portions that correspond to the tibia and femur.
Induction of ectopic eye structures. We used the GAL4 system (12) to target ey expression to various imaginal discs other than the eye discs in which ey is normally expressed. GAL4 is a yeast transcriptional activator that can activate transcription of any gene after introduction into Drosophila if the gene is preceded by a GAL4 upstream activating sequence (UAS) that consists of five optimized GAL4 binding sites ( 12). The GAL4 system is now widely used in conjunction with a method called enhancer detection (13), in which a reporter gene is provided with a weak promoter only and inserted at random sites in the genome by transposition. If the detector has inserted close to an enhancer, the reporter gene is expressed differentially. By isolating a large number of enhancer detection lines, a spectrum of different enhancers with specific temporal and spatial pattems of control can be identified. If GAL4 is used as a reporter gene, these enhancer detection lines can be used for targeted gene expression; the enhancer drives the specific expression of GAL4, which in tum can transactivate a target gene, in our case ey provided with a UAS. As indicated in Fig. 1A, the GAL4 enhancer detection line was crossed to a UAS-ey stock to generate transheterozygous flies that express ey in those cells that express GAL4. We chose approximately 20 GAL4 lines, of which only 3 gave viable adult flies to analyze in more detail (14). The results are illustrated for the GAL4 line E132. When E132 is crossed with a stock containing a UAS-LacZ construct, ß-galactosidase staining reveals the activation of the lac-Z reporter gene by GAL4 and thus the expression pattern of GAL4 in the imaginal discs. E132 expresses GAL4 in discrete regions of the wing and haltere discs, all three pairs of leg discs, and in the antennal imaginal discs (Fig. 1, B through D), which are the primordia for the respective adult structures. When the GAL4 expressing line E132 is crossed with a stock carrying an ey embryonic complementary DNA (cDNA) (15) under a GAL4-UAS control element, transheterozygous flies can be generated, and the expression of ey can be targeted into the imaginal discs as mentioned above (Fig. 1, B. C, and D for LacZ ). In the wild-type controls, ey is only expressed in the eye discs.
Fig. 2. GAL4 driven ectopic
expression of ey induces the formation of eye structures in various tissues. The
sites at which ectopic eyes form correspond to the regions in the imaginal
discs, in which GAL4 is expressed as assayed by the activation of a LacZ reporter construct
(Fig. 1, B. C, and D). The ectopic eye structures show ommatidial arrays,
interommatidial bristles, and red pigmentation (29).
(A) Cuticle of an adult head in which both antennae formed eye structures.
(B) Dissected wing with a large outgrowth of eye tissue. The ectopic eye contains about 350 facets. Many interommatidial bristles are also apparent. The normal eye contains approximately 800 ommatidia. The wing is reduced in size. The anterior margin with its characteristic triple row of bristles occupies most of the circumference, whereas the more posterior structures are absent and replaced by eye tissue. The characteristic venation pattern of the wing is disturbed by the formation of the ectopic eye structures.
(C) Dissected antenna in which most of the third antenna segment is replaced by eye structures.
(D) Dissected middle leg with an eye-outgrowth on the base of the tibia.
As a consequence of ectopic ey expression in line E132, ectopic eye structures were induced in the wings (Fig. 2A), all six legs (Fig. 2B, for mesothoracic legs), the antennae (Fig. 2C), and the halteres. When the flies were raised at 25°C, at which temperature the cold-sensitive GAL4 is properly active, 100 percent of the transheterozygotes produced ectopic eye structures. We observed that the eye structures in the adult cuticles bulged out of the tissue in which they were induced. This phenomenon is illustrated for the wing in scanning electron micrographs (Fig. 3, B and D), and could represent sorting out of heterotypic cells in order to minimize the contact surface between the two tissue types (16). In some cases, the development of the ectopic eyes interfered with pattern formation in the surrounding imaginal disc tissue and resulted in pattern duplications. In the GAL4 line MS941, all of the flies expressed ey in the wing discs and produced eye facets on both wings. In line p339, which expressed GAL4 in a small spot in the wings in low amounts, only red pigment was formed, but again with 100 percent penetrance. We also used a heat-inducible promotor to express ey ubiquitously at various times during development. However, heat shocks during embryonic and most larval stages lead to developmental arrest. To circumvent this lethality, heat-shocks were applied after 80 hours during the middle of the third larval stage. Ectopic eye structures including complete ommatidia were induced. However, targeted ey expression by the GAL4 system was more effective.
Fig. 3. Scanning electron of
ectopic eyes (30).
(A) Scanning electron micrograph of an ectopic eye (arrowhead) in the head region formed by the antennal disc.
(B) Overview of a fly with an ectopic eye under the wing (arrow) and on the antenna (arrowhead).
(C) Higher magnification of (A). The ectopic eye (to the left) contains hexagonal ommatidia and interommatidial bristles. The organization of the facets in the ectopic eye is very similar to the pattern in the normal eye (to the right). Some facets, however, are fused and some irregularities in the fom of the facets are observed.
(D) Higher magnification of the ectopic eye under the wing shown in (B) (arrow). The ectopic eye protrudes out of the thoracic body wall (ventral pleura). The organization of the facets and interommatidial bristles are similar to that of the ectopic eye shown in (C).
The fine structure of the ectopic eyes was analyzed by scanning electron microscopy. Well-developed ectopic eyes were most frequently observed on structures derived from the antennal and wing discs (Fig. 3, A and B). Distinct ommatidia with lenses and interommatidial bristles were seen (Fig. 3, C and D). The array of facets and bristles were largely normal. However, we also observed fusion of facets and irregular spacing of bristles in some cases. The eye structures induced on the legs were on average smaller than the ones on antennae or wings but nevertheless appeared to have a relatively normal organization.
Fig. 4. Histological structure
and differentiation of photoreceptors in the ectopic eye.
(A) Micrograph of a section through an ectopic eye in the antenna (to the left) and the normal eye (to the right) stained with Azur II and methylene blue (15).
(B) Phase contrast micrograph of a section through an ectopic eye on the antenna. The nomlal number and trapezoidal arrangement of the rhabdomeres of photoreceptors is observed in the different ommatidia (arrowhead).
(C) Micrograph of an eye-antenna disc stained with an antibody against the neuronal marker ELAV and a secondary fluorescein labeled antibody. In the normal eye portion (to the nght), regularly spaced ommatidial clusters of differentiating photoreceptors are detected. In the antenna part of the disc (on the left), extensive cell proliferation has led to a doubling in size. In this portion, a large domain of ectopically induced photoreceptors is seen.
(D) and (E) are higher magnifications of (C), which shows the photoreceptor clusters in the ectopic eye (D) and in the nommal eye (E), respectively. An essentially nommal cluster fommation and cluster array is observed in the ectopic eye.
Photoreceptors in the ectopic eyes. Microscopic analysis of sections of ectopic eye structures indicated that the ectopic ommatidia consisted of the full complement of the different types of cells and structures ( 17). In a longitudinal section of an antennal ectopic eye, we were able to distinguish cornea, pseudocone, cone cells, primary, secondary, and tertiary pigment cells, and photoreceptors with rhabdomeres (Fig. 4, A and B). At the base of the ommatidia, we observed the feet of secondary and tertiary pigment cells and a basal lamina that formed a structure with features characteristic of the fenestrated membrane of the retina. On a transverse section, the normal trapezoidal array of rhabdomeres was clearly visible (Fig. 4B, arrowhead).
We also analyzed the neuronal differentiation of photoreceptors by means of ELAV antibodies (18). Clusters of photoreceptor cells were clearly detected at ectopic sites in the imaginal discs (Fig. 4, C and D), and the sequence of neuronal differentiation observed in the normal eye disc was retained in the ectopic eye cells. A number of single cells that expressed the neuronal marker were seen at one side of an ectopic photoreceptor cluster. This expression most likely borresponds to the formation of R8 photoreceptor cells. Subsequently, groups of three, five, seven, and eight cells were detected that expressed the ELAV epitope. This series of events probably corresponds to what is observed in a normal eye disc upon passage ef the morphogenetic furrow. Thus, these observations suggest that morphogenesis of the ectopic eyes is normal and that it probably involves the formation of an ectopic morphogenetic furrow. In summary, the data presented above show that ey can induce the formation of complete and morphologically nomal ectopic eyes. It is unknown whether these ectopic eyes are functional, and whether the axons of the photoreceptors innervate the correct domains of the brain, that is, the lamina and the dorsal deuterocerebrum, respectively (19). Initial evidence suggests that the photoreceptors in the ectopic eyes are electrically active upon illumination (20).
Role of eyeless in eye morphogenesis. The reported findings indicate that ey is the master control gene for eye morphogenesis, because it can induce ectopic eye structures in at least the imaginal discs of the head and thoracic segments. The expression of ey switches on the eye developmental pathway that involves several thousand genes. The number of genes required for eye morphogenesis can roughly be estimated on the basis of the frequency of enhancer detection lines that show reporter gene expression in the eye imaginal discs posterior to the morphogenetic furrow during eye differentiation. Because approximately 15 percent of a large sample of enhancer detector lines fall into this category (21), and assuming that the Drosophila genome contains at least 17,000 genes (22), we estimate that more than 2500 genes are involved in eye morphogenesis. Our results suggest that all of these genes are under the direct or indirect control of ey, which is at the top of the regulatory cascade or hierarchy. The ey gene is expressed first and controls a set of subordinate regulatory genes, including sine oculis, another homeobox-containing gene (23). Subsequently, genes that influence cell-cell interactions and signal transduction must be regulated and, finally, the structural genes like rhodopsin, crystallin, and transducin must be expressed. The lower part of this cascade, including signal transduction pathways, has been elucidated to a large extent (24), but the upper part, and which of these interactions are direct, remain to be determined. However, ey may not only control the initial steps of eye morphogenesis, but also, as suggested from the expression pattern, it may control later steps. Thus, the same transcriptional regulator may be used at consecutive steps of morphogenesis. This could be the consequence of the conservative mode of evolution whereby the same master control gene is used repeatedly to integrate new target genes into the eye developmental pathway. In addition to eye morphogenesis, ey controls other functions in the developing nervous system, because null mutations are lethal, and the loss of eye structures alone is not the cause of lethality.
The induction of ectopic eyes in Drosophila is reminiscent of the classical experiments of Spemann (25) in which he induced ectopic eyes by transplanting the primordia of the optic cup to ectopic sites in amphibian embryos. Our experiments extend these observations and identify the gene that is necessary and sufficient to induce ectopic eyes at least in imaginal discs. In the mouse, Sey is expressed at each step of the induction process; first in the optic cup, then in the lens, and finally in the cornea, which implies that Sey may be the master control gene in the mouse eye induction process (4)
The transformation of antennal, leg, and wing tissue into eye structures by ey induction indicates that ey is a homeotic gene. In contrast to the classic homeotic genes of the Antennapedia and Bithorax Complexes, hypomorphic loss-of-function mutations in ey do not lead to homeotic transformation, but rather, they result in the loss of eye structures. However, targeted ectopic ey expression induces homeotic transformations similar to those observed in gain-of-function mutations of classic homeotic genes, like Antp . Therefore, ey represents a class of homeotic master control genes different from Antp . Gain-of-function mutants with phenotypes corresponding to those obtained in our targeted gene expression experiments have not been discovered previously.
Fig. 5. The ectopic expression of mouse Pax-6 cDNA under the control of GAL4 induces the formation of ectopic eyes (26). The scanning electron micrograph shows a close-up of induced eye facets on a leg. Ommatidial arrays and interommatidial bristles very similar to the ectopic eye structures induced by the Drosophila gene (Fig. 3) were formed (30). In both cases the same GAL4 line E132 was used.
The high degree of sequence conservation between the human, the mouse, and the Drosophila genes, the similarity of the phenotypes of Aniridia, Sey, and ey, and the similarity of the expression patterns suggested to us that ey might be a master control gene for eye molphogenesis that is shared by vertebrates and invertebrates (3). Because we also found homologous genes in ascidians, cephalopods, and nemerteans we propose that ey function is universal among metazoa. In order to test whether the mouse gene can substitute for the Drosophila gene, we also used the mouse Sey gene for targeted expression in Drosophila. Similar to the results obtained for the Drosophila ey gene, the mouse gene Sey can also induce the formation of ectopic eye structures (Fig. 5) (26). As expected, the ectopic eye structures formed contain Drosophila-type ommatidia and not mouse eye structures.
Previously, the function of other mouse homeobox genes has been demonstrated in Drosophila with the use of heat inducible vectors (27). In the case of HoxB6, Drosophila legs were induced in place of the antennae (27). Obviously, the responses, but not the transcriptional regulator, are species-specific.
The observation that mammals and insects, which have evolved separately for more than 500 million years, share the same master control gene for eye morphogenesis indicates that the genetic control mechanisms of development are much more universal than anticipated. It will he informative to compare the regulatory cascade required to form a Drosophila compound eye with that of a mouse eye, to find out what the differences are, and to determine how many new genes have been recruited into these developmental pathways in the course of evolution.
1. M. A. Hoge, Ann. Naturalist 49, 47 (1915); C. B. Bridges, Z. Biol. 4, 401 (1935).
2. B. Hochmann, Genetics 62,235 (1971).
3. R. Quiring et al., Science 265, 785 (1994).
4. C. Walter and P. Gruss, Development 113, 1435 (1991); R. Hill et al., Nature 354,522 (1991); C. C. T. Ton et al., Ceil 67, 1 059 (1 991 ).
5. R. wow and D. F. Ready, in The Deveb,ornent of Drosophila mebnogaster, M. Bate and A. MartinezArias, Eds. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY,1993), chap.22, p.1277.
6. The expression pattern of ey was analyzed by staining of the different mutant eye-antenna discs with a polyclonal rat ey antibody and a fluorescein-bbeled secondary antibody. The mutants have been described in D. L. Lindsley and G. G. amm, The Genorne of Drosophila melanogaster (Academic Press, New York,1992), p.1133.
7. W. McGinnis and R. Krumbuf, Cell 68,283 (1992); J. Botas, Curr. Bid. 5, 1015 (1993); W. J. Gehring, M. Affolter, T. Burglin, Anna. Rev. Biochan. 63, 487 (1994).
8. W. J. Gehring, Science 236,1245 (1987).
9. E. B. Lewis, Nature 276, 565 (1978); M. Kessel, R. Balling, P. Gruss, Cell 61,301 (1990); M. Kessel and P. Gruss, ibid. 67, 89 (1991); P. Hunt et al., Nature 353, 861 (1991); P. Hunt and R. Krumlauf, Annu. Rev. Cell Biol. 8, 227 (1992).
10. S. Schneuwly, R. Klemenz, W. J. Gehring, Nature 325, 816 (1987); G. Gibson and W. J. Gehring, Devebpment 102, 657 (1988).
11. M. A. Kuziora and W. McGinnis, Cell 55,477 (1988); R. S. Mann and D. S. Hogness, ibid. 60,597 (1990); G. Gibson et al, ibid 62,1087 (1990); A. GonzalesReyes and G. Morata, ibid. 61, 515 (1990); A. Gonzales-Reyes et al., Nature 344, 78 (1990); M. Lamka et al., Development 116, 8412 (1992); J. Castelli-Gair et al. , ibid. 120,1983 (1994).
12. A. H. Brand and N. Perrimon, Devebpment 118,401 (1993); J. A. Fischer, E. Giniger, T. Maniatis, M. Ptashne, Nature 332, 853 (1988).
13. C. J. O'Kane and W. J. Gehring, Froc. NaM Acad Sci. US.A. 84, 9123 (1987); H. Bellen et al. , Genes Dev. 3, 1288 (1989); C. Wilson et al., ibid. 3, 1301 (1989); E. Bier et al. , ibid., p. 1273.
14. GAL4-crosses were established at 25°C on standard medium. The GAL41ines were crossed to a number of UAS-ey lines and most of these crosses led to embryonic or espy brval lethality. This outcome is probably the result of the ectopic expression of ey in various tissues dunng embrpogenesis because the GAL4 lines start to express GAL4 during embryonic stages. In crosses with the MS941, p339, and E132 lines, transheterozygote adults were recovered. For MS941, air ost no lethality was observed, whereas for p339 and E132, a substantial number of dead embryos or larvae were noted. For line E132, virtually only females were obtained because most males died dunng the early phases of devebpment. This result may be explained by the dependence of the bthality on the bvd of transadivation of ey by GAL4. In the E132 line, the enhancer detector construct is inserted into the X chromosome and therefore, is dosagecompensated in males. As a consequence, the transheterozygous males produce twice as mudh GAL4 activity as the femabes, and die during larval stages, whereas the femabs survive. Thus, all cutides shown are derived from females.
15. The full-bngth embryonic cDNA was reconstructed in a Bluescript KS+ backbone from three Eco Rl fragments. The full-bngth embryonic cDNA begins with a Hind lil site tat position 45 in the published sequence (3)] and ends with an Xba I site (constructed by inserting Xba l linkers in the Msl I site at position 2741). The cDNA was inserted as an Xho WXba I fragment into the GAL UAS vector [pUAST (11)]. This construction results in an oriented insertion in which the cDNA is proceeded at the 5' end by five optimized GAL4 binding sites, an hsp70 TATA box, the transcriptional start, and the cDNA is folbwed at the 3' end by the SV40 intron and polyadenylation site. A y as w stock was transfomed as described [G. M. Rubin and A. C. Spradling, Science 218, 348 (1982)]. A total of 13 independent pUAST ey strains were analyzed. The heat-inducible construct was made by inserting the embryonic d)NA into the heat-shock Casper vector [V. Pirrotta, in A Survey of Magyar Chants Vectors and 77;teir Uses, R. L. Rodriguez and D. T. Denhardt (Buttesworth, Boston and London,1988), p. 437].
16. G. M. Edelmann,Annu. Rev. CellBiol. 2, 81 (1986).
17. Wild-type and ectopic eyes were dissected and fixed for 30 minutes on ice in a cacodylate-buffered glutaraldehyde-oxmiumtetroxide fixation mixture. After postfixation in cacodylate-buffered osmiumtetroxide, the tissue was dehydrated through an ethanol series and embedded in Spurr medium. One-micrometer sections were cut and stained wrth staining solution (equal volumes of 2 percent Azur II and 2 percent disodiumtetraborate to 2 percent methylene blue). After drying they were mounted with Depex.
18. Staining of imaginal discs wrth ELAV antibodies was perfommed according to S. Robinow and K. White [J. Neurobiol. 22,443 (1991)]. A fluorescein-conjugateJ secondary rat antibody (Cappel) was used. Analysis was done on a Zeiss Axiophot microscope equipped for epifluorescence.
19. 1. A. Meinertzhagen and T. E. Hanson, in 77te Development of Drosophila melangoaster, M. Bate and A. Martinez-Arias, Eds. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1993), chap. 24, p.
20. P. Callaerts, G. Halder, W. Gehnng, in preparation.
21. G. M. Rubin, personal communication.
22. L. M. Hall, P. J. Mason, P. Spierer, J. Mol. Biol. 169, 83 (1983); B. Bossy, L. M. C. Hall, P. Spierer, EMBO 3,2537 (1984); J. Gauze L. M. C. Hall, A. Spierer, P. Spierer, Genetés 112, 65 (1986); J. MirkovHch, P. Spierer, U. K. Laemmli, J. Mol. Biol. 190, 255 (1986). The haploid genome size of Drosopttib is estimated to be 165,000 kb. The gene density can be estimated from the number of transcription units detected on a chromosome walk of 315 kb and amounts to approximately one gene per 7 kb. After subtraction of 22 percent of satellite and other repetitive DNA, we obtain a value of approximately 17,000 genes per haploid genome. This is certainly an underestimate because not all transcripts from this region have been detected, and on the basis of expenence wHh genomic sequencing of yeast chromosomes, a considerably larger value is to be expected.
23. B. N . R. Cheyette et al. , Neuron 12,977 (1994); M. A. Serikaku and J. E. O'Tousa, Genetics 138, 1137 (1994).
24. B. Dickson and E. Haten, in 71&laqno;e Development of Drosophila melangoaster, M. Bate and A. MartinezArias, Eds. (Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY, 1993), chap. 23, p. 1327, S. L. Zipursky and G. M . Rubin, Annu. Rev. NeLtrosci. 17, 373 (1994).
25. H. Spemann, Embryonic Devebpment and Induc-; tion (Yale University Press, New Haven, CT,1938). |
26. The full-length mouse Pax-6 cDNA (a gift of C. Walher and P. Grass) was doned as a Not WXho I Eminent in the GAL-UAS vector [pUAST (11)]. Flies were transfommed as described in (15). To ectopically induce the mouse Pax-6 gene, the UAS-Pax-6 transfommant lines were crossed to the E132 GAL4 expressing line as for the Drosopitib gene (14). The figure shows an ectopic eye on a second leg of a male.
27. J. Malicki et al. , Cell 63,961 (1990); J. J. Zhao, R. A. Lazzarini, L. Pick, Genes Dev. 7, 343 (1993); D. Bachilbr et al. , EMBO J. 13,1930 (1994).
28. ß-Galactosidase staining was perfommed as descnbed in M. Ashbumer, Drosophila, A Laboratory Manual (Cold Spnng Harbor Laboratory Press, Cold Spnng Harbor, NY,1989), protocol 77.
29. For cutide preparatior!s, adults were dissected in phosphate-buffered saline, mounted in Hoyer's or Faure's mounting medium, and immediately photographad to avoid diffusion and bleadhing of eye pigments.
30. For scanning electron microscopy, freshly hatched flies were narcotszed and immersed in 70 pernt atone. After critical point drying, they were mounted and coated with gold. The specimens were observed with a Hitachi S-800 field emission electron microscope at 6 kV.
31. We thank the following colleagues for fly stodes, antibodies, and plasmid vectors: S. Benzer, A Brand, C. Goodman, P. Gnuss, E. Haven, F. Jimenez, W. E. Kalisch, C. O'Kane, V. Pirrotta, C. Walther, and L Zipursky; C. O'Kane and his group for the support of one of us during his stay in the O'Kane laboratory (G.H.); E. Haten and collaborators for advice on histological and antibody staining techniques (to P.C.); and the Interdepartmental Electron Microscopy Servi for assistant in particular A Hetti and U. Sauder; E. Marquardt-Wenger for processing the manuscript; and M. Affolter, W. Keegan, and F. Lossli for critical discussions. Supported by the Colon Foundation of S Leuven, Belgium (P.C.), the Kantons of Basel, and the Swiss National Science Foundation.
1 February 1995; accepted 24 February 1995
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These projects will also appear as pop-ups on the left side of your account Dashboard if you enabled this option.
from Newsletter emails
We send a list of new projects and contests related to the skills added to your profile, so you are updated with opportunities available even if you are not logged in on your Freelancer.com account.
Clicking on the email will take you directly to the project/contest page.
by being a Service Provider
Freelancer Services allow you to complete quick jobs from employers.
1. Click on the My Services tab from your Profile Page.
2. Click Browse More Services to view the available Services.
3. Click on a service to view its details. If you wish to provide that particular service, click Become a Provider.
If you meet the requirements of the Service, you will be added to the pool of service providers. You will be assigned a project when an employer requests for that particular service.
Otherwise, a list of the conditions that you have to meet first will be displayed.
Learn more about Freelancer Services here.
Want tips on how to become a successful freelancer? Read here.

subset: commoncrawl


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Workplace Injuries Lawyers in Massachusetts
More Personal Injury Areas
- Motor Vehicle Accidents
- Pedestrian Accidents
- Railroad Accidents / FELA
- Boating Accidents
- Motorcycle Accidents
- Commercial Truck Accidents
- Medical Malpractice
- Mass Tort
- Insurance Bad Faith
- Wrongful Death
- Class Action
- Drug Litigation
- Workplace Injuries
- Nursing Home Abuse
- Spinal Cord Injuries
- Dog Bites
- Premises Liability
- Products Liability
- Brain Injury
By County
Showing attorneys 1–15 out of 500 attorneys available for your search query.
Michael Stephen Bowser Jr.978-256-2700
Drunk Driving Attorney and Personal Injury Attorney Michael Bowser is a *Board Certified DUI Defense Specialist with a proven record of success. Attorney Bowser excells in drug charge defense and general criminal defense in b... » Read more
National College for DUI Defense
Michael Stephen Bowser Jr.
Board Certified Specialist in DUI Defense Law
If a Massachusetts lawyer is "certified" in a particular area of law by a non-governmental body, the certifying organization is a private organization, whose standards for certification are not regulated by the Commonwealth of Massachusetts. See Massachusetts Code of Professional Responsibility, DR 2-105(B) (1997).
Arinda R. Brooks617-625-4144
Workers' compensation and Social Security disability issues can be complicated if you are trying to legally fight for what you are owed on your own. The Somerville attorney at Brooks Law can make the navigation of those leg... » Read more
Thomas M Kiley978-474-8670
Thomas M. Kiley is the founder and managing partner at Kiley Law Group, LLC. He believes that it is an attorney's obligation to provide clients and their families with aggressive representation and compassionate guidance when... » Read more
Damian Turco866-995-6663
I exclusively handle personal injury matters for Massachusetts clients. My role is to counsel and advocate for those who have been injured as a result of someone else's negligence, be it by a car accident, dog bite, slip an... » Read more
Catherine M. Gallagher508-735-1392
The Law Office of Catherine M. Gallagher,was established in 2006. It is one of the leading general practice law offices in the Worcester, MA, area. Attorney Gallagher was born and raised in the Worcester, MA area. She at... » Read more
Connecticut Bar Association
Catherine M. Gallagher
Board Certified Specialist in Workers' Compensation Law
National Elder Law Foundation
Catherine M. Gallagher
Certified Elder Law Attorney
If a Massachusetts lawyer is "certified" in a particular area of law by a non-governmental body, the certifying organization is a private organization, whose standards for certification are not regulated by the Commonwealth of Massachusetts. See Massachusetts Code of Professional Responsibility, DR 2-105(B) (1997).
Thomas M Kiley Jr.978-474-8670
Attorney Tom Kiley Jr. is a third generation trial lawyer. He is partner at Kiley Law Group, LLC and shares the same passion and dedication to the law as his father and grandfather did. Tom has the ability to help injured vic... » Read more
Jason Sutton508-232-7790
The Law Offices of Sutton & Sutton, PC has a well established personal injury, corporate defense, insurance coverage and workers' compensation practice. The firm is focused on the litigation of civil cases in state and fe... » Read more
Kevin J Mahoney617-492-0055
Attorney Kevin J. Mahoney is a Massachusetts criminal defense lawyer who has won 47 of his last 50 trials, authored a best selling book on cross-examination, and provided on-air legal analysis for CNN, Fox News Live, and Cour... » Read more
National Association of Criminal Defense Lawyers
Kevin J Mahoney
Life Member
Charles S Pappas508-879-3500
Charles S. Pappas is a very experienced and knowldgeable lawyer that focuses his law practice on personal injury cases and, workers' compensation claims. Chuck is a partner at Mahaney & Pappas, LLP in Framingham, Mass... » Read more
Joel H. Schwartz617-742-1170
Joel H. Schwartz, P.C. has been one of the most widely recognized and respected names in personal injury law for more than 50 years. Our partners and associates bring more than 185 years of combined litigation experience to y... » Read more
Adam Peter Phipps781-267-6995
I am both a Registered Nurse and an Attorney, I assist people in the areas of family law (divorce, paternity, modifications, etc...); landlord/tenant claims; and personal injury claims. I also act as an expert witness for oth... » Read more
Jason R Markle508-821-4317
Jason R. Markle joined Keches Law Group Personal Injury Department in 2007. Prior to joining the firm, Mr. Markle worked for the Essex County Superior Court for five years, most notably as a courtroom session clerk. Following... » Read more
Christopher Francis Earley617-338-7400
Boston workers compensation and personal injury law firm.
Christopher Dibella978-327-5140
Nationally recognized trial lawyers handling divorce, personal injury, car accidents, DUI & criminal defense, and a range of other cases in Andover, Burlington, Methuen, and Boston MA. Visit our website to learn more!
Richard C. Hyman617-361-5310
For over thirty years, Richard C. Hyman has been dedicated to obtaining the best possible results for his clients. Clients of The Law Office of Richard C. Hyman will always have direct contact with attorney Hyman and will alw... » Read more


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Series Lake 14 (2017)
Series Lake 14 (2017)
Limited edition fine art prints.
Print on Japanese white handmade washi paper, resistant to aging. acid-free.
Every sheet features four deckle edges, therefore I recommend by framing floating it on top of a acid free mat.
Each piece of artwork is signed and numbered, accompanied by a certificate of authenticity. Limited edition fine art prints with edges are made in the following sizes:
23 x 36 cm/ 9 x 14 paper size / 20 x 27 cm / 7.8 x 10.6 in image size - Limited Edition of 5
33 x 44 cm / 13 x 17 in paper size / 27 x 36 cm / 10.6 x 14 in image size - Limited Edition of 5
35 x 51 cm / 14 x 19 in paper size / 43 x 32 cm / 17 x 12.5 in image size - Limited Edition of 5
Printed on professional printer, an 8-ink dye based system for gallery-quality prints. All images are carefully prepared for shipping, rolled and packaged in tubes for largely prints and box for small prints for secure delivery.
The artist reserves the right to produce up to 2+AF for each artwork in addition to the edition sizes listed.
Shipping Policy Returns Policy


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Collins(49 items found)
CollinsMore and more musicians are looking for instruments and equipment that is affordable but are not willing to miss out on top sound and solid manufacturing. This doesn't have top be a contradiction, just take a look at the instruments by Collins. These fine instruments are distributed exclusively by Musik Produktiv. These instruments are a great alternative for musicians and we have been offering the full Collins range for over 10 years now. All instruments are made to reach our given quality standard and are tested thoroughly before entering the market. E-guitars, acoustic guitars, mandolins, banjos and ukuleles as well as violins, cellos and contrabass and also wind instruments including flutes, saxophones, horns, trombones and trumpets are available from Collins. A range of microphones, DI-boxes, stands and music stands are also on offer.
We support the brand Collins to full strength with over 30 years of experience, know how and servicing.


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View the best solar energy companies currently servicing Ambler, PA
Our 912 reviews of the 46 solar power companies servicing Ambler in 2019 shows you the best 10 local solar panel installation companies by reputation. Enter your zip code and bill into the solar cost and savings calculator above to also see the cost of installing solar with the best solar companies near you and to get free quotes if you wish.
Firstly, no solar contractors can pay to get listed at the top of our search results.
The order of our list of best solar installers is displayed based on a formula balancing reviews number and reviews score only from the last two years. If a company has a lot of reviews and a higher average reviews score, but is below another company in the list, it is because most of their reviews are old and the company above them has more reviews at a better average reviews score in the last two years (even if overall they have fewer reviews or a lower all time average reviews score).
We show all solar energy contractors that have received reviews of solar panel installation work they have done in Ambler even if they don't have an office in Ambler. There are a lot of renewable energy companies in the solar industry in the United States that service areas in which they don't have offices via contract installation teams. Some of these companies receive a lot of complaints (see the big national installers reviews like Vivint Solar, Sunrun and Solar City), but others have really good reviews and so we don't exclude them from our top solar companies lists. They often bring price competition to smaller cities where the local solar companies are so busy they need to put their prices up. It is up to you how much weight you put on a local office when choosing between solar companies.
Services: Solar Pool Heating, Solar Hot Water (Thermal), Commercial Solar Electric, Storage, Residential Solar Electric, Energy Audits
Exact solar did exactly what they said, the price was right and the project was on time. I have saved a ton of money since getting my solar panels installed, I also get $200-$400 back in Srects each month. This is a total win-win for me. Most people think solar is too good to be true, but it's the best. I did have to cut down 2 trees and my house is all electric, with a smaller area of oil heat. So solar was a benefit! Exact Solar also haven't installed my system an gone. If I have a question or concern I can call on and rely on them for help. I can also monitor my system at any time from my android device. GREAT COMPANY... read more
Services: Residential Solar Electric, Energy Monitoring, Storage, Energy Audits, Commercial Solar Electric
Had a system installed last year. Everyone was very pleasant to deal with, and kept me informed every step of the way.
Paradise Energy Solutions
Services: Commercial Solar Electric
Office location: Paradise, PA 17562
Paradise installed our 17 kw ground mount in 2017. Lots of permits for Montgomery County , Md and holdups for inspections. Operational Dec. 2017 and works flawlessly.Produced approximately 21 megawatts to date.Awsome system. Very happy with Paradise. Very honest and professional.
Services: Commercial Solar Electric, Residential Solar Electric
Stephan came for the sales call he is also an installer so he answered all my questions. And I just felt comfortable with them.
See the best offers available in your city
Services: Residential Solar Electric, Commercial Solar Electric
Richard gave me a very good rate on some panels he had received from a lawsuit settlement when one of the big companies went bankrupt without paying him for some of the installation work he did. The installation was timely and professional.
Services: Commercial Solar Electric, Residential Solar Electric, Energy Monitoring, Storage
Held my hand through the entire process, were knowlegeable and honest and always delieverd - on time and on budget.
Services: Commercial Solar Electric, Residential Solar Electric, Solar Pool Heating, Solar Hot Water (Thermal)
Robert and Ed know their stuff inside and out! By stuff I mean every little detail, they take care of it all! PECO made it challenging, at least in my case. Alien, explained all my options, answered every question and provided literature to backup their recommendations. I choose a path and they made it happen in the most economical and effective way. I couldn't be more thrilled! They took care over everything, no sales hype, just straight talk person to person, focused on what worked for me, stuck by and pushed PECO to keep moving, and got it done. If you are looking, be sure you consider Alien Fuel before you sign a contract.... read more
Services: Energy Audits, Residential Solar Electric, Wind Turbines, Solar Pool Heating, Energy Monitoring, Solar Hot Water (Thermal), Storage, Commercial Solar Electric
We did quite a bit of research before choosing Arsenal Solar to install solar panels on our roof. Sam promptly provided a detailed proposal, and he answered all of our questions. He's very knowledgeable about all aspects of solar panels, their installation, and follow up. Sam's estimate was indeed lower than others we received, though that would not have been enough if we weren't convinced that his expertise and the quality of his work were also superior. We continue to have full confidence in Aresenal Solar.... read more
See the best offers available in your city
Services: Commercial Solar Electric, Residential Solar Electric, Storage
Belmont Solar did an excellent job in the selling of their solar panels and a very professional job in the installation of the solar panels on our home. We really liked the price and we especially like the 30% federal income tax credit. They installed the panels in a short period of time and we are very pleased with the operations of the solar panels. We review the operations on a daily basis and we are quite impressed with the power being generated and I would highly recommend this company..... read more
Eternity Energy
Services: Storage, Residential Solar Electric, Energy Monitoring
Eternity Energy installed my system at the end of 2018. I was very pleased with the quoting/sales process and installation was smooth, albeit slightly delayed. I had been thinking about solar for my home for some time and Eternity helped me find a sensible way to do it where I could immediately visualize the financial and environmental benefits off-the-bat. Quality product and sound investment for sure. Good decision on my end - thank you Eternity Energy for the help!... read more
Search by city
Average rating for solar companies in Ambler, PA
Popular solar installation facts
Top 100 residential solar installers 2019
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Best solar companies in Ambler, PA offering quotes online
Highest rated solar companies in Ambler, PA
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Supporting and enabling diversity and inclusion is a business priority at Southern Company Gas. The Office of Diversity and Inclusion partners with the organization to create a work environment where all employees can thrive and feel valued, respected and productive.
Diversity describes the many similarities and differences between people, from life and work experiences, perspectives, and cultures, to race, gender, sexual orientation, socio-economic status, national origin and age.
An intentional approach to inclusion builds a sense of community among our employees, who naturally differ on a number of levels – personality, approach to work, thinking styles, educational background, work experiences, life situations, personal circumstances, expertise, cultural upbringing, family status, etc. Valuing and leveraging those differences as assets to the business creates a unique connection to the Company that supports our organizational goals related to retention and development.
Our diversity and inclusion value is supported by the following program elements:
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Tag Archives: New Nina Bra
Real Talk Interview with Jill M. of Pittsburgh, PA!
To celebrate our recent launch of Aerie REAL we wanted to take the opportunity to feature a few fantastic women who embody #AerieREAL. Our first Real Talk interview is with Jill M., originally from Altoona, PA, but currently living in Pittsburgh, PA and rocking her position as store manger in the Southside Works Aerie Store. Learn more about Jill and what #AerieREAL means to her, her must-have Aerie item, and what inspires her below!
Hi, Jill! Thanks for joining us. 🙂 What is your favorite thing about being Store Manager?
Being able to work with and influence girls that are in collage, especially with the the start of Aerie REAL. I feel like having confidence in yourself is important at this age and I love being a part of building that.
What does #AerieREAL mean to you?
#AerieREAL is being a strong and confident woman…even though naturally we have scars, freckles, back fat, arm hair, and cellulite. And guess what!? Aerie is saying that's okay and that they can relate!
What makes you Aerie REAL?
I'm REAL because I have pale skin (I even have to wear sunscreen in the winter) and I have a lot of freckles!
What is currently playing on your iTunes?
I'm always stuck on "I and Love and You" by the Avett Brothers, anything Fleetwood Mac, and an awesome up and coming band Ancient Ear.
If you could take a vacation to anywhere in the world, where would you go?
Mykonos, Greece – it looks so beautiful and serene!
Where do you see yourself in 3 years?
I would love to be working with American Eagle Outfitters still in the corporate offices or in California! Why California? The warmth!!
Agreed. Pittsburgh right now is not the warmest place on Earth! Speaking of sunshine, what's your favorite new arrival at Aerie for spring?
I love the new Nina Bra! It has a really great fit and I love how the air is all the way through the cup so it looks nice under a t-shirt.
Any quotes you live by?
"Come in with a smile!" and fake it 'til you make it!"
We love those both! Thank you so much for joining us Jill! Show us how YOU'RE Aerie Real and tag your photos with #AerieREAL for a chance to be featured on Aerie.com!


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By Quentin Fottrell
Facebook seems to have soured users as well as investors with its initial public offering, a new poll shows.
Facebook's shares remained down 24% Wednesday from the $38 IPO price of May 18. During that same stretch, the site dropped from No. 1 to No. 4 in the "Customer Loyalty Engagement Index," a measure of brand perception among 49,000 social networkers polled by marketing consultancy Brand Keys. The index now rates Facebook alongside LinkedIn, and behind Twitter and YouTube. "There's no doubt that Facebook's brand was weakened by the events surrounding the IPO," says Robert Passikoff, founder of Brand Keys. "Originally people felt that they were part of a community and now, for better or for worse, they feel like they are part of a business."
As Facebook insiders raked in barrels of cash, and the banks underwriting the deal came under fire for how the IPO was handled, users were left feeling used, experts say. Jim Joseph, a professor of marketing at New York University and an avid Facebook user calls it the "The Facebook IPO Effect." The controversy surrounding the IPO brought back feelings of "mistrust and insecurity" from the last tech bubble, he says. "I hear many people now questioning the long-term viability of Facebook," he says. In fact, an Associated Press/CNBC poll conducted just before the IPO found that 51% of people believed the social network's appeal would fade. Joseph says the ongoing concerns about the share price will only add to that skepticism. "A brand should excel during an IPO," he says.
Facebook aficionados who invested in the IPO have also cooled on the brand. Michael McHale, a New York-based artisan industrial chandelier maker, spent $1,000 on shares. "When I log onto my Facebook, all I think about is the money I lost," he says, noting that he believed the hype would sustain the share price — at least in the short-term. "I don't plan on Friending Steve Wozniak anytime soon — I'd de-Friend him if I could." (Wozniak, a co-founder of Apple, told reporters before the IPO that he'd buy Facebook at any price.) Michael Provitera, a professor of organizational behavior at Barry University in Miami Shores, Fla., who bought $4,000 in shares, agrees. "I'd feel a lot better about the site if I hadn't bought it at this price," he says.
However, others say Facebook's members are too busy updating their statuses and sharing photos to pay attention to the controversy surrounding the IPO. The release last Friday of the photo-sharing app, "Facebook Camera," provided a timely distraction, says Derrick Daye, managing partner at LA-based consultancy The Blake Project. "The IPO issue, while irritating for investors, has less of an impact for users," he says. The biggest threat to the average social networker's loyalty to Facebook will come with a spike in advertising, says K. Jason Krafsky, co-founder of the advice site, The Social Media Couple. He says people will only tolerate a certain amount of change. "Even if the IPO was a great success," he says, "Facebook will have a tough time keeping nearly one billion members happy."
Also See:


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Richard Ward
University of Manchester
(with Steve Pugh (University of Salford) and Liz Price (Hull University))
Keynote Paper: Don't look back? Opportunities for improving health and social care services for older LGB users
Getting older within lesbian, gay and bisexual (LGB) groups or communities is not a well-documented experience and for those in receipt of health and social care services little is understood of what happens in care settings or how needs are managed. Indeed, what has been described as the 'Fourth age' is largely unknown territory for LGB people as few individuals have had the opportunity to report back upon reaching a great age. Questions of what it is like to live with dementia in a care home as a lesbian woman or to negotiate support from a home care agency as a bisexual older man have largely been overlooked by a research agenda focussing upon clinical issues, while the agencies that inspect and monitor the provision of care rarely seek to specifically take account of the experiences of LGB service users. Yet, 'Fourth-agers' arguably have the least capacity for challenging discrimination or ill-treatment of any section of the LGB community. Dependence upon care services can create obstacles to voicing dissent and conditions in care settings are not always supportive of individuals seeking to effect change.
This paper draws upon a review of the literature on health and social care practice and provision in relation to LGB service users in the UK. The emphasis is upon questions concerning opportunities for change to services and how recent developments in policy might provide a context for rethinking how services are delivered and how older LGB users might be taken into account and involved in service development. We argue that long-standing heteronormative conditions, whether localised or national have created a legacy that providers cannot ignore and that younger generations within LGB communities should not forget.


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The American Cancer Society has a lobbying arm called the Cancer Action Network [Read about it here.] The ACS CAN recently launched a big initiative for cancer patients called "Surviving the System". They launched it with a Lobby Day which took place on the 20th.
- Nearly one in three people diagnosed with cancer under the age of 65 has been uninsured since their cancer diagnosis. Scientific research shows that being uninsured can adversely impact your chance of survival.
- One in five families affected by cancer have used up all or most of their savings because of health care costs and more than 40 percent have had difficulty affording their health care costs in the last two years.
- More than two-thirds of Americans under age 65 who tried to find insurance outside their employer couldn't afford a plan.


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Before taking off on a worldwide tour, The Flaming Lips, legendary American rock band and composers of the Playhouse's highly acclaimed musical, "Yoshimi Battles the Pink Robots," will bring down the house in a private unplugged post-dinner performance, followed by late night bites, drinks and dancing.
For guests at the $5,000 commanderie and $2,500 sommelier ticket levels, the Playhouse has also lined up a group of expert wine hosts to curate a journey through their private wine collections with exclusive reserve bottle options at the commanderie level. For information about tickets, which range from $500 to $5,000, contact special events manager Elizabeth Galloway at firstname.lastname@example.com or call (858) 228-3085.


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Amethyst Cat Eye Sunglasses
Designer: BZR
A spin on our best-selling classic round BZR frameless version, these cat eye sunnies are perfect for when you're in the mood for something with a little more drama.
Dimensions: Measures 5.75" in width.
Details: UV protected. Includes case.
BZR began in 2012 when designer Tiffany Ju began experimenting with colorful dyes in her apartment kitchen. From there, she began making accessories for her friends and then launched her line shortly after. She crafts sleek, colorful designs for everyday accessorizing. Made in Seattle, WA.
Always Free shipping on US orders of over $75+
Our team processes orders within 24 - 48 business hours. We are super fast! Just as a reminder - delivery times are from the day your order ships, not always the day your order is placed. Please note that UPS does not count the pick-up day in the delivery time. Learn more about our easy returns here.
Orders are shipped during business hours, Monday through Friday, excluding holidays. Special events and promotions may result in longer processing times. You will receive an email notification as soon as your package is in on the way! Please check to your friend/foe filters to make sure @mooreaseal.com and @shipstation.com emails make it through.
Weather and other delays are outside our control and not covered by shipment time guarantees.
*Additional shipment charges may apply to oversized and/or packages weighing over 5lbs.
**International packages may need additional time for delivery, up to 2 additional weeks, depending on the amount of time needed to clear customs. This amount of time is dependent on the volume of packages going through customs and each destination country's protocols. The customer is responsible for ALL duties and taxes incurred during shipment, which may be required by the courier to release the package. These totals are levied by shipment location's government and are not affiliated or charged by mooreaseal.com. Returned or refused packages will be refunded less any incurred shipment costs and/or unpaid taxes and duties. Please contact your local customs bureau for additional tax and duty information and/or local tax estimates.
Domestic customers: Please note that due to the weight, size, or value of some items or combination of items, we may upgrade your order to UPS. Signature is required for orders over $250.
International customers: Please note that due to the weight, size, or value of some items or combination of items, we may change the shipping carrier in order to avoid an additional shipping charge on your order. We will notify you via email of any shipping carrier changes.
Please ship your package to a secure location or post box to ensure it is delivered without risk of theft. Once a package has been scanned as delivered, theft is outside of our control and as we cannot recoup the value of the package, we are unable to offer refunds or replacements for stolen packages.
If the package has been lost in transit and has not been scanned as delivered, we are happy to place a claim on your behalf with the courier. A replacement or refund will be issued once the courier has finished the claim investigation process.
Orders can be returned for a store credit or refund within 30 days of purchase. Holiday gift returns are accepted through January 10th, 2019 for a store credit.
As of January 14th, due to the rising cost of postage, we are no longer able to offer free return labels. Eligible US domestic returns for orders dated 1/14/2019 or earlier may contact firstname.lastname@example.com for a return label.
Returning an online order or in store order?
Ship your return to our Seattle store with the following information:
- Name and/or Purchaser's Name
- Email Address
- Phone Number
- Date of purchase
- Order Number or Copy of Receipt
- Indicate if you would like store credit, refund, or exchange (exchanges with a due balance will receive an invoice request via email). If exchanging, please make sure to include all the relevant product information for your new item!
Moorea Seal
Attn: Returns
1012 1st Ave
Suite 150
Seattle, WA 98104
*Special Notes:
- Returns must be received within 30 days of your order date.
- Return requests and returns are processed within 5 business days of receipt, during business hours, Monday-Friday.
- We recommend selecting a trackable option for your return shipment. We cannot honor returns that are lost in transit, so trackable options are your best bet!
- All items, including clothing and shoes, must be unworn with no visible signs of wear, and returned with the original box, tags and/or packaging intact. Shoeboxes are not intended for shipment must be returned in the original shipment box or other exterior protective parcel.
- A 20% restocking fee will be applied to any item that is returned without it's original packaging, branded box, or other materials intact.
- Due to the nature of body and consumable products, all body and food items are final sale. We will offer a replacement or a shop credit for certain circumstances. Please send us an email (email@example.com) with any concerns about your purchase so we can work something out.
- Items marked final sale cannot be returned.Late and ineligible returns (worn, beauty, final sale items, etc) will be reshipped back to the customer at the customer's expense.
Email us at email@example.com + someone will get back to you shortly!
If you are returning a gift, please make sure to note this in your return package, provide your contact info (not the purchaser's) and request a store credit. We cannot refund any payment method other than the original purchaser. All gift returns are processed for a store credit.
Refunds are issued to the original payment method (Visa, MasterCard, PayPal, Store Credit, etc). If the original payment method is unavailable or cancelled, the return balance will be issued as a store credit. Original shipment fees are non refundable.
Placing a new order is the easiest way to swap out a style that didn't work for a new one. If your exchange item is available when your return is processed, we will process the exchange as a new order. Exchanges with a due balance will receive an invoice request via email.
We assess repairs and defective item claims on a case by case basis. Please email us at email@example.com with the following information; 1) your order number, 2) the email address used to place the order, 3) name of the item, and 4) a photo of the damage. Please note we are not able to repair or replace defective item claims after 60 days from the date of purchase.
We are unable to cancel or modify orders once they have been submitted. We are sorry about this! Our team is so fast, it is not always possible to catch packages before they are out the door. The good news is that most items are returnable if you decide against them.
Orders that are returned to Moorea Seal because the customer refused to accept the package during a delivery attempt will be processed as a return and a 20% restocking fee will be applied. Refused packages received at our studio within 30 days from the order date will be issued a refund less the 20% restocking fee.
Please ship your package to a secure location or post box to ensure it is delivered without risk of theft. Once a package has been scanned as delivered, theft is outside of our control and as we cannot recoup the value of the package, we are unable to offer refunds or replacements for stolen packages.
If the package has been lost in transit and has not been scanned as delivered, we are happy to place a claim on your behalf with the courier. A replacement or refund will be issued once the courier has finished the claim investigation process.
Please review our Terms and Conditions of use here.


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Minnesota's community radio stations find unique niche for covering arts and culture
Backing from a special tax-supported fund has allowed volunteer-powered stations to enhance their local programming.
Current (https://current.org/current-mentioned-sources/loren-mayor/)
Backing from a special tax-supported fund has allowed volunteer-powered stations to enhance their local programming.
Representatives of stations told FCC officials that allowing sales of the educational spectrum could create a "hostile leasing environment."
OETA's former fundraising foundation has threatened to "destroy" membership data it claims to own.
The president's fiscal year 2020 budget marks his third attempt to end funding for CPB.
Confirmed board members include a former ambassador and a member of the RNC executive committee.
Ron Hetrick has run finance and administration at WITF since 2009.
How KPCC used design thinking to extend the reach and impact of its coverage of early childhood education.
Moss Bresnahan has led both WTVP-TV and Illinois Public Media since 2014.
A Catholic diocese plans to sell its two radio stations for $1.25 million.
The lifestyle and how-to programs will be available on Amazon's Prime Video Channels.
Ashley Bailey is reporting this week for "After Paradise," a show airing on North State Public Radio.
The longtime host delighted classical listeners for more than four decades.


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Welcome to the Comfort Inn Manhattan, your home away from home. The Manhattan Motel is a family owned and operated business with a proud history of providing quality accommodation at affordable prices. Whether you are visiting Adelaide for business, pleasure, or just need a night away, the Comfort Inn Manhattan has a variety of rooms and amenities to suit your needs. Our newly renovated rooms offer a bright and modern decor, complimentary WiFi and easy parking at your door. In addition to our excellent facilities, friendly staff and warm hospitality, we promise to do our best to ensure that your visit is as pleasant and enjoyable as possible.
Thank you for choosing the Comfort Inn Manhattan
This historic Barn built in 1842 has all the modern luxuries while keeping its rustic charm. At 'Marybank', experience the layers of history within it's beautiful interior. Treasured memories await in a very intimate, relaxed and secluded environment. Gourmet breakfast provisions are provided as well as a bottle of Sparkling wine & local treats.
Save $40 per night when you escape mid week (excluding public holidays) and still enjoy the exclusivity of this property.
Welcome to Accommodation Ingle Farm, we offer superb holiday accommodation with our professional team, locate your provider today from our extensive range of listings below.


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Maribel1 needed a new logo design and created a contest on 99designs.
A winner was selected from 1029 designs submitted by 170 freelance designers.
A Forever Home Rescue Foundation (AFH or A Forever Home)
We're an all volunteer 501c3 non-profit, all breed dog rescue located in Northern Virginia. We started as a small group but our numbers have grown to 1600 volunteers. We have had the current logo since our inception, so we would like to update our image to reflect the maturing of our group and need your help to freshen it up and bring it up to speed in 2010.
We are looking for a simple, clean, and sophisticated look. Since the name of our organization, A Forever Home, can be mistaken as a home builder, realty group, or housing related organization, we want to make it clear that we are a dog rescue.
Our target audience is, of course, potential adopters in the Washington DC Metro area. However, we also want to attract support in the form of sponsorship and donations from businesses and the local community. Since we also deal with other animal related issues like shutting down hoarders and puppy mills, we are often given spots on TV and other media outlets.
Our logo needs to be flexible enough that it can be used on banners, varying printable material, T-Shirts, our Vehicles, and Website. We are completely open to color schemes or even single colors. We do not need it to have a house in the design but want the logo to give the viewer a warm, homey feeling or a happy feeling. Most of all, we want to stay away from a cartoon like logo, which is what our previous logo was like. Also no cats, please--we love them but that is not our bag.
Also, we need the winner to provide the file in a vector format, preferably in .ai format.
Find out how a design contest works or learn more about our logo design services.


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Moving 6 tons of sand by hand: It's all in a day (or five days) of river research for UB scientists
Release Date: March 19, 2013
BUFFALO, N.Y. — The work wasn't glamorous, but it had to be done.
Last November, University at Buffalo master's student Ethan Mamer removed 6 tons of sand — by hand — from a 10-meter-long (33-foot-long) artificial river he was using to study how water cycles through streams.
The purpose: Mamer needed to empty the river to repair leaks in silicone caulking that were affecting experimental results.
"It took some thought as to how I was going to get all the sand out and where I would put it," Mamer said. "We toyed with the idea of getting a giant moving dolly, a complicated pulley system and another couple of plans that were all a little crazy."
In the end, he settled on simpler tools: shovels, 55-gallon barrels and a lot of muscle. The job took five days. Several students in Mamer's department, geology, took pity on him and volunteered to help. Then, in January, after plugging the leaks, the team spent four more days refilling the sand (video here: http://www.youtube.com/watch?v=X2iCAPee5PY).
Mamer ran another series of experiments. They worked. He was euphoric.
Welcome to the world of river restoration research.
Though scientists in the field spend countless hours studying real-life streams, scale models made from lumber and PVC pipes also play an important role in helping investigators understand rivers.
At UB, scientists are using such devices to test techniques for strengthening riverbanks, and to simulate groundwater discharge in streams — a process that plays a role in removing nitrogen pollution and supporting wildlife.
These model waterways, called flumes, are awesome apparatuses that feature flowing water and elements customized to meet the needs of research projects. They require maintenance (as Mamer discovered), but the science they support is important.
Details on two flumes at UB, both built by the UB College of Arts and Sciences Instrument Machine Shop:
Groundwater Simulator
Research Team: Ethan Mamer (MS student, geology), Chris Lowry (assistant professor, geology)
Flume Location: Natural Sciences Complex Basement, UB North Campus
Flume Features: This flume pipes water into a thick bed of sand to simulate how groundwater bubbles up to the surface of streams. Heat-sensing fiber-optic cables run through the flume, providing Mamer with temperature measurements that he uses to determine how groundwater, which is cool, is rising and mixing with warmer surface water.
The body of the flume is a 5-meter-long rectangular box, but a divider runs lengthwise down the center, doubling the length of the artificial river to 10 meters.
Research Purpose: Mamer and Lowry recently completed a series of experiments using the flume to model how groundwater and surface water interact. This work enables the scientists to verify their field results and improve techniques for locating groundwater hotspots in real rivers — important information for stream restoration.
"In zones where groundwater is discharging, you have more invertebrates, which are a food source for fish like trout," said Lowry, Mamer's faculty advisor. In addition, the cycling of water from the surface of a river into the ground and back can help remove nitrogen pollution, which enters streams as agricultural fertilizer runoff.
Stream Restoration Test Bed
Research Team: Donghua Cai (MS student, geography), Michael Gallisdorfer (PhD student, geography), Seyed Mohammad Ghaneeizad (PhD student, civil, structural and environmental engineering), Sean Bennett (professor, geography) and Joseph Atkinson (professor, civil, structural and environmental engineering)
Flume Location: Statler Commissary, UB North Campus
Flume Features: This flume is a 7-meter-long, 2-meter-wide scale model of the Big Sioux River in South Dakota. Using a battery of instruments that slide along movable guiderails, scientists can measure the volume and speed of water flowing through the flume, along with the forces that the water exerts on structures placed inside the flume.
The "river" drains into a large plastic basin, where it's then recycled — pumped through white PVC pipes back to the top of the apparatus. On a recent afternoon, water was moving through the flume at a rate of 425 gallons per minute.
Research Purpose: Placing formations of engineered log jams into rivers can protect stream banks from the erosive forces of flow, and Bennett and his team are studying which formations work best: "What should they look like, where should they be placed, and what should be their frequency in space?" he said.
To test different stabilization structures, the researchers place models of the structures into the flume and track how their presence affects turbulent flow, fluid forces and bed topography.
The team's findings will help scientists working with the City of Sioux Falls and the South Dakota Department of Environment and Natural Resources make decisions on how to stabilize the real Big Sioux River, Bennett said. Ghaneeizad says one of the most exciting parts about the flume work is that it will yield real-world results: "This is a project that will be used somewhere in the United States. The results will help someone — it's not just a library project."
Media Contact Information
Charlotte Hsu
News Content Manager
Sciences, Economic Development
Tel: 716-645-4655
Twitter: @UBScience
Pinterest: UB Science


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Write an Android assignment
1. Implement a native Android application
1.1. Has at least two different kinds of views in your Layout (TextView, EditText,
ImageView, etc.)
1.2. Requires input from the user
1.3. Makes an HTTP request (using an appropriate HTTP method) to your web
1.4. Receives and parses an XML or JSON formatted reply from your web service
1.5. Displays new information to the user
1.6. Is repeatable (I.e. the user can repeatedly reuse the application without
restarting it.)
2. Implement a web service, deployed to Heroku
2.1. Using an HttpServlet to implement a simple (can be a single path) API. It is
recommended that you do not try to use JAX-RS / Jersey but rather create the
API in the style you did for Project 3.
2.2. Receives an HTTP request from the native Android application
- 3 -
2.3. Executes business logic appropriate to your application. This includes fetching
XML or JSON information from some 3rd party API and processing the response.
§ -10 if you use a banned API
§ -10 if screen scrape instead of fetching XML or JSON via a published API
2.4. Replies to the Android application with an XML or JSON formatted response.
The schema of the response can be of your own design.
Alternatively, you can adopt a standard schema that is appropriate to your
application. (E.g. Common Alerting Protocol if your application deals with
emergency alerts.)
12 威客就此工作平均出价 $107
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Hi I'm ready for this project. I'm expert in Android development. Please give me an opportunity. I will do it perfectly. Best Regards VnNorthStar
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text:
Resources >> Browse Articles >> Nursing Trends
Resources >> Browse Articles >> On the Job
Should Nurses Blow the Whistle?
Jennifer LeClaire | Monster Contributing Writer
With the various corporate scandals of recent years, we've seen plenty of headlines about whistleblowers in the business world. Now, the nursing industry is bringing us its own front-page cases that could see more medical watchdogs come to the fore.
Legal experts say the number of whistleblower cases in the healthcare field has been on the rise since 1999, when the Institute of Medicine of the National Academies reported that medical errors are the nation's leading cause of death and injury.
"Nurses are becoming more vocal about concerns that healthcare organizations are using unlicensed assistant personnel and not employing enough nurses on a shift," says attorney LaTonia Denise Wright, RN, of the Healthcare Risk Aversion Group in Cincinnati. "This is a real concern, because these practices lead to medical errors."
What's Your Health Care IQ?
Nurses Speak Out
Nurses are also blowing the whistle on questionable practices. Cindy Moore, RN, got vocal about the way Florida's Duval County Health Department diagnosed people with sexually transmitted diseases. She complained that health officials were not notifying infected people in a timely manner and was subsequently fired for her accusations.
Likewise, Stephanie Hohman, RN, blew the whistle on the University of Texas Medical Branch (UTMB) emergency room when she became concerned about patients' rights and possible abuse. Hohman was also fired. And psychiatric nurse Stacie Neldaughter, RN, was fired after reporting alleged misuse of shock therapy treatment at a Wisconsin hospital.
Despite the career risks, healthcare attorneys say nurses have a responsibility to blow the whistle on such activity.
"Everyone has a moral obligation to blow the whistle on unsafe practices," says attorney Joanne Sheehan of Friedman, Newman, Levy, Sheehan and Carolan in Fairfield, Connecticut. "A nurse may be disciplined by the state licensing board for participating in unsafe practices that can harm patients."


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The United States Sports Academy selected Brandon Spradley from more than 100 applicants for one of five doctoral teaching assistant positions in August 2011.
One year later, the 24-year-old found himself in Malaysia on a three-week teaching assignment with 20 other university instructors involved in training 1,000 of Malaysia's physical education teachers on the latest techniques and sports science for youth sports.
Besides teaching, Spradley joined an Academy delegation that met with Malaysia's Deputy Prime Minister and Minister of Education Muhyiddin Yassin. Spradley briefly hobnobbed with him during introductions. The moment showing a beaming Spradley was captured on camera by the Malaysian media and printed in newspapers throughout the country and across Southeast Asia.
"It was definitely a big deal," Spradley says, laughing. "People looked at me like a star."
The Academy, also known as America's Sports University, began delivering its International Diploma in Physical Education and Scholastic Sports (IDPESS) program June 20. The six-month program approved by the Malaysia Ministry of Education (MOE) is taught in six different regions throughout the country. The Academy's faculty developed the 10-course diploma program to teach in Malaysia, where the nation's leaders want to enhance the physical education programs taught in its schools and to improve youth sports.
Spradley is a former University of Alabama track and field sprinter who earned a bachelor's in Kinesiology in December 2009 and earned his master's with honors in Exercise Science in December 2010. He spoke with The Sport Update about his positive experience in Kulim, Malaysia.
The Sport Update: What did you think about the chance to teach physical education in Malaysia?
Brandon Spradley: First off, I didn't think I would get an opportunity like this. I feel so blessed to have this opportunity at the Academy to go overseas for the first time in my life. As far as the teaching experience was concerned, I had a great experience. The Malaysian teachers we were training were knowledgeable about the subjects and were really interested in learning more. They asked a lot of questions about P.E. and sports in the United States. In Malaysia, most of them only taught students twice a week for 30 to 45 minutes. They want to change up their curriculum. Teaching there gave me a big confidence booster in my ability.
The Sport Update: Did you have a highlight?
Brandon Spradley: Kulim was a beautiful city depending on what area you were in. We did do a little sightseeing but not much because we had long days teaching. My highlight, though, came in the second course. The purpose of it was teaching social and physical responsibility through P.E. If you learn values through physical education they may transfer to other areas of life, like being respectful at home. Dr. Tim DeVinney, Dr. Fred Cromartie and I had our four classes create three events and compete in them like a Malaysian Olympic Games. They had to come together and create the rules and regulations. We also had opening and closing ceremonies, fireworks and sang their national anthem. It was a lot of fun but also matched up perfectly with what we were trying to teach in this course.
The Sport Update: What did you like about Malaysia?
Brandon Spradley: There are a lot of different cultures and nationalities in Malaysia. They all seem to get along just fine. It seemed there was a lot of togetherness there and a lot of pride in their country. There were few African-Americans but I was able to fit right in and they treated me like a regular guy. We were teaching there during the Muslim celebration of Ramadan, where they wouldn't eat or drink during the day. We just wanted to be respectful of the students and not eat in front of them but the class wasn't too affected by us. We didn't have to adjust too much to their culture, we just had to act respectful.
The Sport Update: What was it like getting to meet Malaysia's Deputy Prime Minister and having your picture in many of the country's papers with him?
Brandon Spradley: The students were really interested in it. They were like, "We saw you on the news!" They gave me newspapers and translated them for me. The reason I was smiling so much was that the DPM said, "You must be the youngest." I didn't expect him to make a joke and thought it was funny. That was definitely a big deal. People looked at me like a star.


text:
Busha Browne's Pukka Hot Sauce
Produced in Jamaica, this condiment is made from crushed Jamaican Scotch Bonnet peppers. The 2nd Place Winner in the Chile Pepper 2003 Fiery Food Challenge for the Hot Sauce-Chile Specific division, this spicy serum adds zest to steak, chicken, fish and is great in creole and cajun cooking!
Happy H'eating!
Postal Address
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You can also receive a catalog from us by giving us a call at, 336 596 0454.
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text:
Items where Author / Artist / Editor is "Dai, Donghai"
|See full list of headings
Group by: Item Type | No Grouping
Jump to: Article
Number of items: 1.
Cole, Laurence A. and Dai, Donghai and Butler, Stephen A. and Leslie, Kimberly K. and Kohorn, Ernest I. (2006) Gestational trophoblastic diseases: 1. Pathophysiology of hyperglycosylated hCG. Gynecologic Oncology, 102 (2). pp. 145-50. ISSN 0090-8258


text:
s_hanson_99 needed a new logo design and created a contest on 99designs.
A winner was selected from 41 designs submitted by 19 freelance designers.
The singles ministry of West End Community Church in Nashville, "Metro", needs a new logo to kick off a new year and a new branding effort.
Metro is the singles ministry of West End Community Church in Nashville, TN. WECC is associated with the PCA (Presbyterian Church of America) denomination. Metro is a ministry of WECC that serves singles between the ages of 25 - 34. The ministry provides bible study and social activities for singles to get involved and plugged into the church and surrounding community.
This is a new year for Metro after 2 years of poor performance. I am looking for a new logo to signal to the public that this is a new ministry, a new beginning and something to get excited about. I chose the word "Metro" for a couple of reasons: 1. Nashville is referred to as "Metropolitan Davidson and Williamson Counties" and 2. I wanted the ministry to have a broad focus outisde the walls of WECC. Reaching into the whole of Nashville.
I'm looking for something I can use for signage, correspondence, and other marketing materials promoting the ministry to the congregation and public.
I wish I could offer more prize money, but I am unfortunately limited by very tight church budget.
- Do whatever you want, but it must use the word "Metro"
- May or may not encorporate the phrase(s): "Singles Ministry of West End Community Church" or "Singles Ministry" or "Singles Ministry of WECC" or any variation or combination of the words. It's not necessary though.
- no vulgarity
Find out how a design contest works or learn more about our logo design services.


text:
Royal Caribbean International (Royal Caribbean) has notified the Commission that due to the fire it experienced this weekend, the company has cancelled the next six sailings of Royal Caribbean's GRANDEUR OF THE SEAS. The ship is expected to return to service with the July 12 sailing.
Royal Caribbean has informed the Commission that it will provide passengers of the canceled cruises with the following:
Guests onboard the May 24, 2013, sailing of Grandeur of the Seas will receive:
Guests booked on the May 31, 2013, sailing of Grandeur of the Seas will receive:
For any sailings that may be canceled beyond May 31, 2013, Royal Caribbean will provide affected guests with:
Royal Caribbean International's Vice President of Government Relations, Eleni Kalisch, informed the Commission that passengers with questions or concerns should contact Royal Caribbean International's hotline for guests on the canceled sailings at (888) 824-0012 in the US or +1-408-916-9000 internationally. Royal Caribbean has also posted information on its website about the incident and the actions it is taking to assist guests scheduled to cruise on the Grandeur of the Seas.
Questions or concerns may also be directed to the Commission's Office of Consumer Affairs & Dispute Resolution Services at (202) 523-5807 or firstname.lastname@example.com.
This site is an official U.S. Government Website


text:
Commercial Carpet Cleaning in Bloomfield, New York
- Our friendly, skilled staff is trained and well equipped to clean carpets at any major facility........stores, schools, churches, medical facilities, offices, nursing homes, day care centers, etc.
- We provide flexible scheduling, whether you need a one time job done, bi-weekly, weekly, monthly, you name it, we'll do it.
- Our rates are the most competitive you'll find in Bloomfield NY, for the quality of service and product.
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Thomas Carhart, Janitorial Services2018-05-30


text:
The first Met I remember as a kid was Ed Kranepool. Maybe it was the way Bob Murphy pronounced his name, I don't know. Who really knows why things stick in your head when you're ten years old?
My family spent our summer vacations at my grandmother's house in Pelham, and I watched a lot of Met games. This was before the 1969 season, and they usually lost, often in agonizing fashion.
Kranepool always stood out although he wasn't a great player. At the time, he was pretty much the best the Mets had to offer.
By 1979, I was following the Mets in the box scores and occasionally the Game of the Week. Growing up near Cleveland, the Indians were on once or twice a week, and I always thought how great it would be to live in New York when the games were on every day.
On this date that season, Kranepool hit the 118th, and final, home run of his career in a 3-2, 15-inning win over Pittsburgh.
Kranepool made his debut as a 17-year old in the Mets' inaugural 1962 season as a defensive replacement for Gil Hodges, Sept. 22, and the next day started his first game and collected his first hit.
He began the next season splitting time at first base and right field, and was getting more time the following year. In 1965, he gave up his No. 21 to Warren Spahn and began wearing No. 7, and was the Mets' lone representative in the All-Star Game.
Kranepool was demoted to Tidewater in 1970 and contemplated retirement, but had his best season the following year. He lost his starting job in 1973 to John Milner, and was a platoon player the next two years, and finished his career as a role player/pinch hitter, retiring at 34 in 1979.
After retirement, Kranepool was part of a group that attempted to buy the Mets, but lost out to the Nelson Doubleday-Fred Wilpon group. He worked as a stockbroker after retirement and was inducted into the Mets' Hall of Fame in 1990.


text: Hello I've installed PowerDNS on a Virtual Machine and on another I've installed his recursor but when I uncommented the recursor configuration in pdns.conf, the DNS didn't work. My network is in IPv6-only, and I'm also trying to have an access on the website from a customer computer connected in PPPoE (IPv6 + DNS), but the DNS failed (DNS request time out with nslookup) DNS => fd01:1:1:120::53 DNS Recursor => fd01:1:1:120::54 Website => fd01:1:1:120::81 pdns.conf Code: daemon = yes distributor-threads = 3 do-ipv6-additional-processing=yes guardian = yes launch = mysql local-address= local-ipv6= fd01:1:1:120::53 local-port=53 module-dir=/usr/lib/powerdns query-cache-ttl=300 recursive-cache-ttl = 150 recursor= fd01:1:1:120::54:5400 setuid=pdns recursor.conf Code: aaaa-additional-processing=yes allow-from= ::/0 local-address=fd01:1:1:120::54 local-port=5400 query-local-address6= fd01:1:1:120::54 Thanks. Best regards.


text:
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