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In 2013, the Garda detected the recently convicted darknet weapons seller, Michael Andrew Ryan. Two years later, US law enforcement linked the online identity known as Brad Jones and Gunrunner. He faced 18 various weapons related charges—and they consisted, mainly, of selling Glocks, Berettas, and Tauruses on the darknet. He sold to Ireland, England, Scotland, and Australia, court documents explained. He committed some fraud too; he purchased handguns under false pretenses, officials said.
And while the court documents claimed the Manhattan, Kansas, man sold to those countries, Ryan ended up admitting that the charges were accurate. He accepted a plea deal and pleaded guilty to only six charges in exchange for four years in prison. A senior Garda spokesperson told news agencies that the Garda "welcomed the jailing of a gun dealer in America after he was convicted of gunrunning to several countries, including Ireland."
As we followed the case from the very beginning, the depth of the investigation became quite clear. And I need to point out that Gwern made the initial discovery. He read between the lines on court documentation and connected the dots. He posted his findings on Reddit and the story has not left circulation since. The depth of the investigation became the highlight for the Garda; for instance Ryan removed the serial numbers from many of the weapons.
The Garda used advanced forensics and restored the numbers, then the numbers went to the ATF who managed to track down the original location of the gun – excluding the manufacturer. This case "showed that Garda had the capacity to work closely with international agencies to combat a growing problem of gun dealing on the Internet, the spokesperson said.
In addition to working with the ATF, the Garda worked hand-in-hand with Irish Customs. In addition to preventing illegal weaponry from landing in the wrong hands, law enforcement needed as many recovered serial numbers as possible. The more serial numbers they obtained, the closer law enforcement moved towards the source.
Customs intercepted the disassembled guns that Ryan shipped in various inconspicuous objects. Court documents mentioned "Mexican statues," but we have not seen pictures of any such object or similar item yet. Neither Irish Customs or the Garda commented on how they knew about the operation. They explained, in brevity, that the guns came from the internet on "special websites that used secret browsers" but little else.
Police have not arrested any suspected recipients, a Garda source said. Given that the investigation began in 2013, suspected buyers may remain free. The police believed that common criminals bought the guns—not any organized militant group.
Other countries involved received or intercepted fewer shipments of guns; Australia made no mention of US collaboration.


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February 12, 2010
Happy Valentine's Day (a tad early!)
Gretchen was trying to figure out how to put a heart shape around photos, but could only manage an oval. It works, though doesn't it? You can really see how these two — Saber and Gemma — love each other even without having to look into their eyes.
They set the tone for the Friday before Valentine's Day. There was actually a lot of love to go around, though we had to dodge the raindrops in order to spread the love today.
Our day had some twists in it that I wasn't expecting and I'm not talking about the weather either. First, we left school this morning and picked up Woobie. "Woobie?" I questioned Gretchen. "Why Woobie?" She informed me that the schedule got changed and Woobie would be our first walk of the day. Of course, that's when it started to rain. Not hard, but definitely enough that by the time we stopped by Monty's house to pick him up, we were pretty wet.
But then the rain subsided and the three of us walked to the park to the tennis courts.
Woobie escorted us home where we posed for a photo on the porch. The dark clouds were really moving in so you can imagine what was coming…more rain!
Monty and I got brunch while Woobie braved the rain and headed next door to pick up Oshi and Perrito. By then the rain was really coming down so the only chance Gretchen got to take a photo was under the cover of Woobie's porch.
Oshi and Perrito got quite the rub down when they got home, but by the time Gretchen walked over to Gemma's house, the rain stopped again. Just in case, Gemma had to wear her raincoat, though. They headed toward the park and even though Gretchen knew they'd stop by Saber's house, Gemma pulled her there regardless. I'm telling you, this is true love.
It's a good thing Saber is all skin, isn't it?
And it's a good thing he wears out quickly otherwise Gemma would run him into the ground…well, it looks like she did!
Gretchen was going to take another photo of the two lovebirds on their walk, but every time she went to get her camera out of her backpack, the two of them threw themselves at each other. It was safer for everyone to avoid another photo op!
Those two went home tired and will no doubt dream of each other tonight while Gretchen headed home to pick us up. And guess who decided to join us? Jessica and Quillette! See, another twist, but that wasn't all. We walked up to Bradner Gardens and when Gretchen marched up the long stairway to a familiar house, I knew we were in for yet another twist — Chloe!!!
We haven't seen Chloe for over a year. We generally only walk her when her Mom's off skiing, but the weather hasn't produced much snow here (sorry to the other Washington!) and so not many ski trips can be planned. But today we got a real treat as all four of us (and two humans) walked down to the lake and practiced sitting as close together as we dared on that high up dock!
I have to say, I have a special place in my heart for that Chloe. Can you tell?
We walked back up through Mount Baker park and when we got to the top of the hill we could see gray, gray clouds painted on the horizon. Would we make it home before the clouds opened up on us?
To be concluded…
…just kiddin! Chloe got home alright and we made it back to our house just as the rain started pelting the ground. Whew! I'm going to be away for a few days and dog blog will pick up again next Wednesday. I'm off to hopefully recuperate from my intestinal distress and Gretchen will be faithfully by my side administering love, massages, and something called acidophilus. Is that like a Greek Dog or something?
Happy Valentine's Day everyone!

subset: commoncrawl


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I know, I have already posted and drooled about Thomas O'Brien, but now, I have to blab about his new book. It's out and I can't wait to get it. If you haven't pick up the April issue of Canadian House & Home, you don't want to miss it. It has an article on O'Brien's apartment; a before and after story. Only the before is just as gorgeous as the after!!! I really can't get enough of his vintage studio styling. To tantalize the tastebuds, here the cover shot of "American Modern" and more snaps from Canadian House and Home....
Gorgeous chair.
The low cabinets fronting the bookcase is a great look, piled with interesting books and objet d'art.
Mmmm....I really have to collect more art.
On another note, I have been thinking about transforming Elizabeth's room to some sort of functioning work area (for sewing, folding laundry, etc) and have decided this vintage studio look is the way to go. I nearly went nuts a couple days ago, spending way too much time online looking for a daybed or sofa bed that would work in such a room. You have already seen the desks and rugs I like (here), when I was seeing more of an office. I reduced the daybed/sofas down to these:
This one (above) is my favourite, by Innovation USA
This one is the Flex Sofa by CB2
And for a little more inspiration: I love the look of zebra hide rugs, but can't bring myself to support that industry (no Anna Wintour am I), but how tantalizing to the eye it is.
Domino via Decor8
Living, Etc & Domino pics via Decor8
Unfortunately, the printed zebra rugs don't have the authentic, beautiful look. I think I may be able to find fabric and do an ottoman like the one above. A couple days ago, I picked up this zebra dish from Pier 1 Imports. Cute, isn't it? $6 in the US/$9 CDN (should have waited for my trip to Syracuse next month).


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George Town police detectives are looking for two masked men that robbed a Walkers Road store Thursday afternoon. The 911 Emergency Communications Centre received a call from a staff member of Shop Right at around 1.30pm reporting that two masked men had robbed the store. Police responded to the scene and learned that two men had entered the store carrying what looked like a knife and a sword. The men demanded cash from the clerk, who was working alone in the shop, and after receiving an undisclosed sum of money they left. The men were wearing jeans and dark coloured tops and had masks over their faces. One of the masks was described as a Halloween type of mask, the other was a Spiderman mask. No-one was injured in the incident.
- Advertisement -


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5 Lessons Learned: Experts
Situations When You Need A Car Accident Lawyer
At least once for every eighteen years, an average American is filing claims for collision. Well, that may not sound so alarming considering the fact that many motorists are driving on a regular basis. Before hanging up their keys, drivers can expect to be involved in at least 3 car accidents. Again, if we are going to consider the number of cars that are running in the US, this figure isn't so shocking at all.
According to statistics, there are actually more registered passenger cars in the US than to any other countries. And because there are many cars and that Americans drive greater distances than other nationalities, it is inescapable for them to be involved in accidents. Believe it or not, there are approximately 10 million recorded car crashes annually which range from fender benders to some fatal accidents.
Most of the time, only property is what damaged during these collisions but 1 out of 5 accidents or around 2 million per year, someone gets hurt. The increasing medical costs together with continued presence of older drivers lead to more injury claims as well as greater possibility that a lawsuit would be filed in civil court. The following is a short list of common car accident cases.
Property damage – no matter who is the one at fault in the accident, as long as neither party was hurt or even injured, you should settle it in short order. Opposing insurance adjuster will likely offer you same settlement a lawyer can obtain for you in these open and shut cases. Having said that, there's no need to get a personal injury lawyer or law firm.
Minor injury cases – according to law, an injury is deemed minor if you can recover fully after few days and costs less than a thousand dollars in medical bills. These cases normally involve contusions, scrapes and cuts. The victim can be forced as well to miss a couple of days from work but no more than that. Since the settlement barely cover the bills, it is not necessary to call a car accident lawyer for these kinds of cases.
Major injury cases – it's recommended that you contact a lawyer in case that the damage was too big and has caused you to miss more than 2 days of work. Some of the common injuries here are whiplash, joint damage, head trauma or broken bones. It is important to immediately contact a car accident lawyer when any of the injuries stated are sustained due to the risks of having permanent impairment.


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Design beautiful websites and apps easily, using top-notch components built with an extreme attention to details.Download DemoBuy Full Version
Version 1.9.1 requires Sketch 52+
Frames toolkit helps to save hundreds of hours on every project by making the full use of the latest Sketch app app features and best practices.
Use the power of Symbols and Shared Styles to create beautiful designs with grace at high speed.
All building blocks are made using Nested Symbols, which gives tons of customization options to each separate component.
Use native resizing to freely change the size of any individual element, compositions or entire layout.
All symbols are linked with the style guide, any changes made are staying in sync with the UI kit providing full consistency for your project.
Frames includes a collection of pre-designed layouts sorted into popular web categories that are ready to be used as working materials for kick starting a new project in seconds.Preview in Sketch Cloud
Frames contains a set of 50 Charts + 3 Table templates that can be easily customized, resized and reused to help you focus on working with data instead of vector points.
Lightweight and simple symbol structure
Helpful to get started with Sketch app
Two Type systems: Roboto and Muli
Made with Bootstrap in mind and 1140px grid
Easy to share and export to Web apps
Save up to 60% of time working in Sketch
Constantly growing and updatable toolkit
Learn best practices and useful technics
Pay once, get free updates forever.
For personal use
For team use
We send email notifications and tweet when the new version is released, also, see the Version History log. If you already bought Frames just go to the Product page and download the latest version.
You can get a 50% discount on all product, if you are a student or a teacher, to enroll for an educational discount just e-mail us any proof of your occupation.


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We do not buy our products from industrial distributors, we cooperate with artisans.
We got delivered pastries, breads as well as fresh orange juice and fruit salad each morning.
A farmer provide us with his jams (over 25 flavors ) according to seasons and crops.
Several options are available :
The buffet in the beautifully decorated breakfast room, room service or at the bar for an express formula .
Our Continental breakfast is served from 7:00 to 10:30 from monday to friday and from 7:30 to 10:30 on the weekend


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Lego Universe Cracked Server
Kal Online Top 1. Top 1. 00. Private servers and game sites. Lineage. 2, Kalonline, Minecraft, World of Tanks, Ragnarok Online, 2. Moons, Priston Tale, RF online, World of Warcraft, Knight Online.
Play as all of the characters from the movie, including Rey, Finn, Poe Dameron, Han Solo, Chewbacca, C- 3. PO, and BB- 8, as well as Kylo Ren, General Hux and Captain Phasma.
Furk. net : : Furk. You can use it to stream video or listen to your music from PC, smartphone, HTPC or even a game console (XBOX, PS3).
Nonton dan download video bokep indo terbaru, video ngentot, video sex, video mesum, mulai dari bokep sd, bokep smp, bokep sma, sampai mahasiswi. Registrar Corp is an FDA compliance agency that provides regulatory assistance for companies in the food and beverage, medical devices, cosmetics and drug industries. Gratis Voetbal Spelletjes Downloaden Voor Ipad.
Comparez les meilleurs tarifs d'assurance auto, moto, animaux, maison, obs. TurboBit.net provides unlimited and fast file cloud storage that enables you to securely share and access files online. Livengrin Foundation is open 24/7 to respond to request for information or admissions for drug and alcohol addiction treatment and substance use and abuse. Kal Online We list the best Kal Online sites on the net in more tan 55 categories. Links to various fun gaming sites, private servers, including mmorpg, flash, java. Koleksi bokep terbaru, koleksi bokep indo, bokep asia, bokep barat, download bokep gratis, kumpulan bokep terbaru, bokep webcam, gadis bugil, bokep sd.


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yes my speaker of pc is not working.....................
If you have NOT loaded Windows from scratch since the speakers last worked, the headphones jack on laptops has a tiny mechanical electrical switch inside of it that disables the built in speakers from producing sound when a plug is plugged into the headphones jack. . If you have used the headphones jack a lot, it's common for that mechanical switch to malfunction. If you insert a plug into that jack and wiggle it, then pull it out, that may get one or both built in speakers to produce sound again, but the only lasting solution is to replace the headphones jack - laptop repair places do that frequently.
OR - if you plug a pair of amplified computer speakers into that headphones jack, that will probably yield you sound.
If you HAVE loaded Windows from scratch since the speakers last worked, the drivers for your sound adapter are probably not built into the contents of the Windows CD.
Most Dell laptops I've seen that originally had XP on them came with a Dell XP Re-installation CD and a Drivers and Applications CD or DVD or similar when the computer was new.
If you have the Dell Drivers and Applications CD or DVD or similar, install the software for your sound adapter from that.
If you don't have that Dell CD or DVD, you must go to the Dell web site and find the software downloads for your specific model and download and install the sound software and install it properly.
While you're at it, you should install the main chipset drivers for your model, and after that the drivers for anything else listed in Device Manager that it shows you do not have the drivers installed for.
(e.g. RIGHT click on My Computer - Properties - Hardware - Device Manager.)


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Come join us for the first St. Louis Revit User Group Event of 2018!!
Our topic for this event is Visualization. Everything from simple Graphic Override techniques in Revit to a fully developed VR/AR model using a game engine. We are going to discuss the gamut of possibilities for visualization of models created in Revit. We plan to break these techniques down into a menu of approaches based on the effort and expertise required to provide different levels of visualization for your clients.
The event will be on Thursday, February 8th 2018, starting at 6 PM and ending at 9 PM. The venue for this event is the HOK St. Louis Office at 10 South Broadway (Suite 200), St. Louis, 63102. See below for parking/access information.
You can park in the HOK (Stadium East) garage. To take the skybridge over to the building go to the 6th Floor of the garage. You can also park in the Ball Park Village Parking lot if desired.
HOK's office is located on the 2nd Floor, we will have people allowing access to the floor or you can go through the main lobby on the 1st Floor, but you may need to sign in as a guest at the security desk. The elevator access will allow people to get to the 2nd Floor on their own until 6 PM.


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Understanding the Tile Pyramid
Using a distributed framework built on the Apache Spark engine, Aperture Tiles enables you to create a set of data tiles that summarize and aggregate your large-scale data at various levels in a interactive, pyramid-style visualization.
Tile Pyramid Hierarchy
At the highest level in the tile set pyramid (level 0), a single tile summarizes all of your data. On each lower level, there are up to 4z tiles, where z is the zoom level (with lower numbers indicating higher levels). At each level, the tiles are laid out row-wise across the base map or plot, starting at the lower left.
Each tile summarizes the data in the region of the base map/plot to which it corresponds. Tiles are serialized objects partitioned into a variable number of bins (typically 256 x 256 for heatmaps). Each bin contains an aggregation of all the data points in the tile partition it represents.
Tile Generation Process
The process of generating a set of tiles from your raw source data is called a tiling job. The tiles created by a job can be served and browsed in any modern Web browser.
As shown in the following diagram, the tile generation process has five main stages:
- Raw Data Parsing: Pass in your raw data and parse its structure.
For standard tiling jobs, this is the main task; the rest of the applicable stages are kicked off automatically.
- Bin Analytics: Create summaries per tile that are partitioned into bins.
- Extract intermediate values from the records in your data (e.g., a count of values and their sum).
- Map to one tile bin.
- Aggregate all the values in each bin (e.g., divide sum by count to get mean value).
- Output the final value written to each bin (e.g., mean value).
Example Use: Create heatmaps distributed across each zoom level in your tile pyramid.
- Data Analytics (Optional)
- Extract intermediate values from the records in your data.
- Map to one tile.
- Aggregate all the values mapped to each tile (e.g., determine the maximum individual raw data value).
- Output tile metadata values.
Example Use: Create custom tile overlay analytics. Not part of the standard tile generation process.
- Tile Analytics (Optional)
- Extract additional data tile data.
- Map to one tile.
- Output tile metadata values.
Example Use: Analyze completed tiles and store information to tile metadata (e.g., extract the minimum and maximum bin values, which are used to apply color ramps).
- Tile Creation: Assemble the values generated by the various analytics into a completed tile pyramid, first in memory and then to HBase or the local file system.
You can execute the tile generation process on your source data in any of the following manners:
|Job Type
|Uses the built-in CSVBinner tool, which automates generation of tiles that aggregate numeric data by summation or take the minimum or maximum value.
|A set of properties files that describe the source data to analyze and the job options to pass in.
|Uses a set of RDDBinner APIs for creating custom tile-based analytics on non-numeric or non-delimited data.
|Create custom code for some or all of the tile generation stages. See the Twitter Topics demo (tile-examples/
|Uses the built-in CSVGraphBinner tool, which automates generation of tiles that aggregate node and edge data.
|A set of properties files that describe the source data to analyze and the job options to pass in.
|Third Party
|Use third-party tools. This approach is not discussed below.
|Adhere to the Aperture Tiles Avro schema. Basic schema files are in the Aperture Tiles source code (binning-utilities/
Before you run a tiling job, make sure you meet all of the prerequisites listed in the following section.
Tiling Job Prerequisites
Aperture Tiles uses a distributed framework built on the Apache Spark engine to generate tiles. Before you run a tiling job, you must install and configure the following prerequisites:
- Scala v2.10.3
- Apache Spark v1.3
If you intend to work with datasets that cannot fit in the memory of a single machine or if you wish to avoid wait times, we recommend you also install your preferred Hadoop distribution to enable Aperture Tiles to work with particularly large datasets:
- Cloudera v5.4.7 (recommended)
- Apache
- MapR
- HortonWorks
For detailed information on Aperture Tiles prerequisites, see the Installation topic.
To configure Apache Spark for your installed version of Hadoop
Perform one of the following actions:
- Download the correct version directly.
- If no version is listed for your flavor of Hadoop, build Spark to support it.
The Aperture Tiles source code relies on the standard spark-submit script to build your tiles. The script simplifies the process of running Spark jobs by including all the necessary libraries and setting various parameters.
Next Steps
For details on running standard tiling jobs, see the Run Standard Tiling Jobs topic.


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What is Freemasonry?
Freemasonry is a fraternal organization that simply seeks to improve men so that they may, in turn, improve society around them. It makes good men better. But it does not tell them how to do it, nor does it give them political, commercial or religious instructions.
Freemasonry is the oldest, largest and most widely recognized fraternal organization in the world. Current worldwide membership totals 3.6 million members, 1.6 million of which are in North America.
As a fraternal organization, Freemasonry unites men of good character who, though of different religious, ethnic, or social backgrounds, share a belief in the fatherhood of God and the brotherhood of mankind.
The traditions of Freemasonry are founded upon the building of King Solomon's Temple, and its fraternal ceremonies use the working tools of the stonemasons to symbolize moral lessons and truths. For example, Masons are reminded at Lodge to "meet upon the level of equality, act by the plumb of uprightness, and part upon the square of virtue."
Like most organizations, one will get out of Freemasonry what he is able to put into it. However, membership in Freemasonry is not meant in any way to interfere with an individual's commitment to his faith, family, or occupation. Freemasonry is not and never can be a replacement for these important institutions, but rather it is a positive environment that reminds every Mason of his duty to God, his community, his family and himself.
Freemasonry provides opportunities for sincere, honest, forthright men who believe in God and desire to contribute to the improvement of their communities and themselves. Through our Masonic Fraternalism, we reaffirm our dedication and unity to become involved citizens who have a strong desire to preserve the values that have made and continue to make America great..


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Price growth has begun to stall in 2011. The residential markets around Australia have been impacted by rising interest rates, and the decline in first home buyers and general purchaser activity upon the expiry of the First Home Owner's Grant Boost Scheme.
Economic activity has also slowed in 2010/11, although private investment is poised to take over from waning Government spending as the key driver of the economy, and growth is expected to accelerate from 2011/12. Moreover, most residential markets remain in deficiency, with low vacancy rates and solid rental growth coming through. Interest rates have been relatively stable in 2010/11, but are in line to edge upwards again to fend off re-emerging inflationary pressures.
Can the improving economic conditions underpin further price growth or will affordability constraints hamper sentiment? Will investors continue to return to the market as the economic outlook improves? To what level will first home buyer demand recover in 2011 once the current decline after "pull forward" effect of the Boost Scheme is worked through? What does this mean for the price outlook over the next three years by capital city?
Residential Property Prospects 2011-2014 provides a comprehensive review and the outlook for residential property in each capital city and includes price forecasts for the regional areas of Cairns, Townsville, Sunshine Coast, Gold Coast, Newcastle and Wollongong through to June 2014. Understand what is happening with the key fundamental drivers of demand and supply:
- the economic outlook and business conditions
- interest rate movements and timings, housing loan affordability
- impact of Federal and State government incentives
- net interstate and overseas migration
- underlying demand and dwelling construction
- stock deficiencies or oversupply in each market
- trends in rental yields and forecasts for rental growth
- annual median house price forecasts for capital cities and above regional areas
An annual subscription includes the main report (published June), along with an Update report in December. Your organisation will also have access to our team of residential property consultants and analysts throughout the year to further discuss forecasts, content or methodologies. Subscriptions are for a hard copy of the report only.
Residential property is the major investment for most Australians. But without the information that allows you to judge correctly when to buy and when to sell, you can lose badly.
The report...
• forecasts annual median
house prices to June
2014 for capital cities
(Sydney, Melbourne,
Brisbane, Adelaide, Perth,
Hobart, Canberra andDarwin).
• also provides forecasts
for selcted regional areas
(Newcastle, Wollongong,
Gold Coast, Sunshine Coast,
Townsville and Cairns).
• forecasts annual rental
growth for residential
• forecasts economic
conditions, interest rate
movements and timings
• provides forecasts for
net overseas and
interstate migration.
• estimates both underlying
demand and pent-up
demand or excess supply
for each city.
• explains the residential
property cycle.
Who should subscribe?
• Developers and builders
• Private investors
• Real estate agents
• Financial institutions
• Investment advisers
• Government departments
and agencies.


text:
The next face-to-face meeting of RESNA Standards Committee on Support Surfaces will be held October 25-26, 2017 in Reno (Sparks) Nevada at the Nugget Casino Resort. Meetings are open to anyone; voting on documents requires membership.
The RESNA Standards Committee on Support Surfaces last met in April 20-21, 2017 in Station Park, Farmington Utah at the Weber State University Farmington Station Campus to discuss assistive products for tissue integrity when lying down and test methods for full body support surfaces for characteristics related to tissue integrity.
The work of the committee will impact persons that use hospital beds, manufacturers of hospital beds and support surface overlays, home healthcare companies that distribute this type of equipment, hospitals, long term care facilities and other entities that purchase and use this type of equipment, and insurance companies and government organizations that pay for this type of equipment.
RESNA is a standards developing organization accredited by the American National Standards Institute (ANSI). The RESNA Assistive Technology Standards Board is the U.S. Technical Advisory Group to ANSI for the development of ISO (International Organization for Standardization) standards pertaining to assistive technology and other products for persons with disabilities.
It is important to the work of the committee that we have participating members from various aspects of expertise in evaluating the characteristics of full body support surfaces. This includes manufacturers, users, clinicians, distributors, researchers, suppliers, and testing organizations. The committee is currently looking for users, distributors, and testing organizations.
If you have any questions about the work of this committee or how to get involved, please contact Evan Call at email@example.com or Kristen Thurman firstname.lastname@example.com for additional information.


text:
The smallest bones in the body are found in the middle ear, and there are three of them: the incus, malleus, and the stapes, otherwise known as the anvil, hammer, and the stirrup.
Sound travels at the speed of 1,130 feet per second, or 770 miles per hour.
Thirty-seven percent of children with only minimal hearing loss fail at least one grade.
A 12-year study conducted by the neurology department at Johns Hopkins School of Medicine found that untreated hearing loss increased the risk for dementia.
The inner ear is no larger than a pencil eraser in circumference.
Crickets have their hearing organs on their knees, male mosquitos hear with thousands of tiny hair cells growing from their antennae, and snakes hear through their jawbones.
Individuals with hearing loss wait an average of seven years before seeking help.
Your sense of hearing is dependent on tiny hair cells housed inside the inner ear. Damage to these hair cells results in hearing loss.
Skin glands in the ear canal produce earwax, which helps protect the ear by lubricating it and cleaning it of dirt and dust.
The ears are not only necessary for hearing; they also help with balance.


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Do you want to play and download The Tribez for PC or on your laptop? On Windows or MAC? More inches and better controls! Find it below!
Category: Simulation
Developer: Game Insight
Requeriments: Android 4.0.1
Official link Game
Features of The Tribez game!
Spring bunnies have hid their Easter eggs on different islands! Find them and complete the special spring collection of decorations in the Rarity Collector's Wonder Reel.
Complete all the festival's tasks and get a Spring Big Ears as a gift! Game Insight, the Best mobile game publisher of 2012, presents its legendary game The Tribez! The Tribez is not just a game where you build a farm! The Tribez is an adventure where you travel to a distant past full of secrets, mysteries, and many hours of addictive exploration of an unknown, but beautiful world! You are invited to visit a primitive world inhabited by a peace-loving people, who have been hidden beyond a miles-deep portal from time immemorial.
Build your own stone-age village, explore territorieshidden by mountains and seas, farm fertile lands, and lead your tribe, which considers you sent by the gods, to prosperity.
The Tribez is a world in which you feel alive! REVIEWS: The Tribez is the insanely popular empire-building game thats revolutionizing the idea of social gaming. (http://www.thedailybeast.com/) The design is so beautiful and there is so much detail that it's a real pleasure to navigate through the scenes and help the villagers. (http://www.appszoom.com) There is tons of content, quests, and things to accomplish in the game. (http://www.pocketfullofapps.com) What makes The Tribez genuinely pleasant to play is the attention paid to the graphics. (http://www.gamezebo.com) KEY FEATURES: ? This game works when youre offline too play it on a plane, in the subway or in the car.
Enjoy! ? Simple, intuitive controls.
? Adorable characters youll love! Meet the farmer, the builder, the tax collector, and many others! ? A beautiful vibrant world you'll be immersed into instantly.
? Lively animations make the prehistoric world come to life.
Youll be mesmerized by how the crops growon the farm and how the builders work on construction sites.
? Tons of items, characters, buildings, and decorations.
? Truly inexhaustible possibilities to develop your own stone-age empire by producing goods, increasingyour population, expanding your borders and attempting to farm the best lands.
? Hundreds of captivating quests: search for treasure and artifacts, discover distant islands, explore mysterious caves, grow unusual crops on your farm, and much, much more! The game uses in-app purchases.
In-app purchases can speed up gameplay.
Official Page on Facebook: https://www.fb.com/TheTribezCommunity Official game trailer: http://www.youtube.com/watch?v=ZoXWVgCHrSs Discover new titles from GameInsight: http://www.game-insight.com Join our community on Facebook: http://www.fb.com/gameinsight Subscribe to our YouTube channel: http://goo.gl/qRFX2h Read the latest news on Twitter: http://twitter.com/GI_Mobile Follow us on Instagram: http://instagram.com/gameinsight/ Privacy policy: http://www.game-insight.com/site/privacypolicy
Instructions for play The Tribez on PC
Many players who have downloaded and play to The Tribez on PC with control or with keyboard and mouse have improved their stats, so you do not stay behind, here you have the solution to improve.
- First you will have to install an emulator of the Android (Download link below) operating system in your PC, laptop or MAC.
- After the installation and run emulator, configure it and add your Google account, remember "@gmail".
- Once everything is OK, just open the Play Store Market and Search The Tribez mobile game.
- Make sure it is the correct game, there are always similar applications.
- Once the installation is over, Tap the App icon in Menu to start playing.
- If is everything OK, Enjoy!
- Configure your new controls on PC, laptop or MAC.
- Destroy your opponents!
Tutorial play and download The Tribez for PC, Windows or MAC
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Below is the solution for Petty fight crossword clue. This clue was last seen on Feb 24 2018 in the Thomas Joseph crossword puzzle. While searching our database we found 1 possible solution matching the query "Petty fight". Please check the answer provided below and if its not what you are looking for then head over to the main post and use the search function. You can always go back at Thomas Joseph Crossword Puzzles crossword puzzle and find the other solutions for today's crossword clues.
Petty fight crossword clue


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About the Icelandic people
The Assential Bob Dylan
Bob Dylan smells issues
– the Assential Bob Dylan
A while ago, mr Dylans neighbors complained about the smell that came from his toilet (read more about it here).
I thought it was so funny, that I decided to write down as many jokes regarding this as possible, by using his own song titles.
Here's the list (hold the mouse cursor over the title, to see the original one):
- "Don't stink twice, it´s alright"
- "Knocking on heaven´s odeur"
- "Maggie´s fart"
- "Poo' boy"
- "Shit ain´t me, babe"
- "Slow drain"
- "Like a rolling stool"
- "You're gonna shit me"
- "Brownsmell girl"
- "Buckets of drain"
- "If you sewer, say hello"
- "Bob Dylan's 115th cream"
- "A moonbeam on your feces"
- "Tight connection to my fart"
- "Poo right to me baby"
- "Shit hurts me too"
- "Tell me that it isn´t poo"
- "I threw it all your way"
- "Don't ya smell Henry"
- "Someone's got a hold of my fart"
- "We better take piss-cover"
And believe it or not – I'm 34 years old! 😀


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I love paranormal and historical romance novels. I love talking about books and finding out about new authors and books to read. I also love supporting and promoting my favorite authors.
Please feel free to check out my blog @
I was provided this book through the 2016 NERFA Contest put on by the First Coast Romance Writers. I have not read or heard of this author until I was provided a copy, so I went in not expecting much. I was wonderfully surprised. I loved it. The characters are strong, the plot is well developed, the conflict is strong, and the passion was perfectly hot (pun intended). Brooke is a strong heroine and Levi is a passionate hero. The story had highs and lows, some humor, action and adventure, and some emotional turmoil that kept me turning the pages. I very much enjoyed this book and will definitely be keeping an eye out for a book 2.


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Adventure Games Description
Adventure is wrapping you in its arms and from there is where fun begins. Let see if you can handle these awesome adventure games. Good luck!
new racing games, shooting games, car games, bike games and sport games are added weekly!
Copyright 2017 Brightest Games
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Democracy settles differences between competing visions of public life, so after every election there are inevitably happy winners and unhappy losers.
Under our traditional "50%-plus-1" majority rule, a whopping "50%-minus-1" losing minority could feel jilted after an election, which is an awful lot of unhappiness within a community. Rarely are ballots evenly split, so the unhappy losing camp is usually far less than half the number of voters.
Here in the US, mythic birthplace and bastion of 'majority rule,' we sometimes intentionally allow a minority to win on election day. This was possible in LASD a couple years ago when we voted on Measure E, the school parcel tax increase. If just one third of voters had voted against Measure E it would have failed, since a two thirds supermajority is required for such tax measures. (Luckily, Measure E passed, but only just barely.)
Allowing a one-third minority to overrule majority sentiment is like political leverage; each No vote cancels out two Yes votes.
Parcel tax measures require a two-thirds majority Yes vote, and so can be particularly difficult to pass, because even semi-organized opposition can be hard to overcome even if it isn't richly funded. But "one-third/two-thirds" is the farthest we stray from majority rule. We would never dream of allowing a 10% minority camp to win over a 90% majority. That would be tyranny of a tiny minority.
If someone's rights are at stake, it's an altogether different story. "Majority rule" is suspended when fundamental rights are on the line.
We recently learned this (again) through our experience with California's Prop 8 and the ensuing legal fight over marriage rights and equality. When the courts ruled that marriage is a fundamental right of all adults, including homosexuals, then even overwhelming majority voter sentiment in favor of outlawing same-sex marriage is disallowed. Even a 99% majority opinion is insufficient to deprive someone of fundamental rights.
How does this relate to charter law? I think it's important to consider whether we want local school districts to be governed democratically or whether anyone has rights that trump majority rule.
Q: Does any child have a fundamental right to a seat in a 'choice' school if their democratically-run district doesn't want to operate 'choice' schools? Is 'choice' a fundamental right in California public education?
A: No.
Then on what basis is a school district's authority usurped by a county Board of Education in re: charter petitions? If voters in a local school district prefer a consistent, high-quality educational program across all its schools, and if the schools are successful by government measures, on what grounds are local voters' ballots nullified by an external agent of government?
This is most definitely the situation we are in today in Santa Clara County.
Through the years and for a wide variety of reasons having nothing to do with remediating poor education, tiny minority groups have created (or threatened) a charter petition to extract from the district that which it was not otherwise willing to provide:
- Los Altos School District: "Trustees closed our neighborhood school and consolidated our student body with another at a different campus. We parents don't agree with the Trustees' decision, so we want to create a charter school. The charter's location, size, curriculum and student body will all be the same as the neighborhood school that was closed."
- Palo Alto Unified School District: "Trustees refused to create a Mandarin language magnet program in response to parent requests. The district offers a Spanish language immersion program and we want a similar Mandarin program. Since the Trustees won't accommodate us, we'll start a MI charter school."
- Sunnyvale Elementary School District: "Trustees denied parents' request to expand the district's K-5 magnet program to middle school grades. We love our magnet program and don't want our kids to be forced out and into district middle schools. If the district refuses to expand the program we will form a charter school."
In all cases, district Trustees elected by voters to balance the needs, priorities and resources of the entire district must decide whether modifying their educational program to suit a tiny group is justified. If they refuse the demands of these groups, they instead face a secessionist charter petition. The group might be just 2% of voters in the district or 5% of households with school-aged children, but in all cases, CA charter law is used to force the hand of district Trustees. That's huge leverage. That's tyranny of a tiny minority.
If a district board denies a charter application, the applicants can appeal to the County Board of Education, and unlike the locally elected District Board, a County Board of Education is prohibited by law from considering what impact the proposed charter would have on the remainder of the district's students.
In other words, upon a district's denial of a charter application and its appeal to a County Board, the petition evaluation methodology is completely different. By law, County Boards need not, and cannot, balance and defend the rights and priorities of the entire district community, as an elected district Board quite obviously must.
County Boards may ONLY consider the merits of the charter application in light of the students to be enrolled in the charter, so a tiny minority can put their own interests ahead of everyone else in their district by simply appealing a charter denial to their county Board.
In this way, California's charter law is used to deny equal protection to the majority of public school students in district schools after a charter group secedes. Charter law is being deployed by county Boards of Education to strip control of school districts from democratically elected local Trustees, overriding the majority will of district voters to serve the demands of a few.
That's just wrong.


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Its starting to actually feel like spring here in NY. That means spring cleaning and shopping for some new spring essentials.
After spring cleaning out my closet, I realized I don't have any white jeans that fit me. So, that was going to be my top priority on my most recent shopping trip.
After hitting, Target, The Gap, Banana Republic and Old Navy, I found the perfect pair at JCrew.
They fit perfectly and were on sale. Winning!
While I was there, I tried on this top. It was petite and although it fit, I wasn't comfortable with the length of it. I wanted it a bit longer. So, back on the rack it went.
Last summer I was in a car accident nothing major but my black sunglasses were destroyed. I've been looking for an inexpensive pair to wear everyday to replace them and I found them!! They were on super sale too!
They are half black half tortoise. I know I will be wearing them all the time!! I love a successful shopping trip where I went looking for something specific and I found it! Now mama is off to get a mani/pedi. Happy Spring!


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- Methodology article
- Open Access
A simplified but robust method for the isolation of avian and mammalian muscle satellite cells
- Belinda Baquero-Perez1Email author,
- Suresh V Kuchipudi†1,
- Rahul K Nelli1 and
- Kin-Chow Chang†1
© Baquero-Perez et al.Licesee BioMed Central Ltd. 2012
- Received: 15 February 2012
- Accepted: 11 June 2012
- Published: 21 June 2012
Current methods of isolation of muscle satellite cells from different animal species are highly variable making inter-species comparisons problematic. This variation mainly stems from the use of different proteolytic enzymes to release the satellite cells from the muscle tissue (sometimes a single enzyme is used but often a combination of enzymes is preferred) and the different extracellular matrix proteins used to coat culture ware. In addition, isolation of satellite cells is frequently laborious and sometimes may require pre-plating of the cell preparation on uncoated flasks or Percoll centrifugation to remove contaminating fibroblasts. The methodology employed to isolate and culture satellite cells in vitro can critically determine the fusion of myoblasts into multi-nucleated myotubes. These terminally differentiated myotubes resemble mature myofibres in the muscle tissue in vivo, therefore optimal fusion is a keystone of in vitro muscle culture. Hence, a simple method of muscle satellite cell isolation and culture of different vertebrate species that can result in a high fusion rate is highly desirable.
We demonstrate here a relatively simple and rapid method of isolating highly enriched muscle satellite cells from different avian and mammalian species. In brief, muscle tissue was mechanically dissociated, digested with a single enzyme (pronase), triturated with a 10-ml pipette, filtered and directly plated onto collagen coated flasks. Following this method and after optimization of the cell culture conditions, excellent fusion rates were achieved in the duck, chicken, horse and cow (with more than 50% cell fusion), and to a lesser extent pig, pointing to pronase as a highly suitable enzyme to release satellite cells from muscle tissue.
Our simplified method presents a quick and simple alternative to isolating highly enriched muscle satellite cell cultures which can subsequently rapidly differentiate into well developed primary myotubes. The use of the same isolation protocol allows better inter-species comparisons of muscle satellite cells. Of all the farm animal species investigated, harvested chicken muscle cells showed the highest percentage of muscle satellite cells, and equine muscle cells presented the highest fusion index, an impressive ≈ 77%. Porcine cells displayed the lowest amount of satellite cells but still achieved a modest fusion rate of ≈ 41%.
- Muscle satellite cells
- Primary skeletal muscle cultures
- Immunocytochemistry
- Desmin
- Pax7
- α-sarcomeric actin
- Fusion index
Skeletal muscle cells are complex multi-nucleated structures that specialize in contractile movement. During the prenatal period of muscle development, embryonic myoblasts initially increase in number (hyperplasia) by mitosis, later align and fuse into large post-mitotic multi-nucleated cells, myotubes, which further mature into contractile myofibres. Subsequently, post-natal/post-hatch development of muscle cells is characterised by an increase in the size of myofibres (hypertrophy), accomplished in part by the addition of myoblasts to the myofibre sarcolemma[1]. Myoblasts are derived from muscle satellite cells (a population of resident stem cells), which reside between the basement membrane and the myofibre sarcolemma[2]. When the muscle tissue is subjected to injury, stretch or exercise, muscle satellite cells are activated, divide and differentiate into myoblasts[3–5]. Similar to the embryological development of muscle, myoblasts undergo repeated mitosis and fuse into a pre-existing myotube or form new myotubes.
Several methods have been described for the isolation and in vitro culture of muscle satellite cells from skeletal muscles to study various aspects of skeletal muscle biology. An early method to isolate muscle satellite cells from rat skeletal muscle was described in 1974[6]. Since then, a wide variety of modifications have been carried out to isolate satellite cells from other species such as human[7], chicken[8], turkey[9], horse[10], cow[11], sheep[12] pig[13] and rabbit[14]. In essence, all these methods involve common or similar steps which are mechanical mincing of muscle, followed by enzymatic digestion to release satellite cells, and differential centrifugation to separate the satellite cells from muscle debris. Following centrifugation, freed cells are plated onto culture flask coated with muscle extracellular matrix proteins such as collagen type I, gelatine, laminin or fibronectin. If further enrichment of muscle satellite cells is required, cells could be pre-plated on uncoated culture plates to remove faster adhering fibroblasts or centrifuged through Percoll gradients[8] which is more time consuming and labour intensive.
Primary cultures derived from skeletal muscles are often mixed populations of muscle cells and non-myogenic cells such as adipocytes, immune cells (e.g. macrophages) and particularly fibroblasts. Primary cell cultures are an invaluable tool because they are obtained directly from a normal animal, do not contain tumour cells unlike immortal cell lines and more closely represent in vivo muscle cells. Primary skeletal muscle cultures have been used in various studies involving development of new medical applications[15–18], better understanding of muscle physiology and even production of in vitro meat[19, 20].
Most of the approaches that have been used to date for the isolation and culture of satellite cells do not usually include characterization of types of cells in culture. Studies conducted with an excessively heterogeneous cell population may not accurately provide valid insights into the biology of skeletal muscles in vitro. Identification of primary muscle cells can be achieved either by morphological features of the cells or by analysing the expression of muscle-specific genes. While the multi-nucleated myotubes in culture could be readily identified, a significant proportion of mononucleated cells cannot be distinguished from non-myogenic cells. Hence, identification of muscle-specific marker proteins by immunocytochemistry is valuable in distinguishing myogenic and non-myogenic cells in culture.
In addition, most of the methods described for the isolation of primary cultures of skeletal muscle are time consuming and complex. There are no standardized methods that could be used to isolate satellite muscle cells across a variety of different species. Hence, there is a need for a simple, rapid and reproducible method that will generate a high yield of muscle satellite cells from different species. We report here on the development of a simplified protocol that can be used for the rapid isolation and identification of primary muscle cells from chicken, duck, horse, cow and pig skeletal muscles. We found this method to be cost efficient and yield high number of primary muscle cells that readily fuse to form myotubes in all the species tested.
Results and discussion
Key known factors which affect the isolation of satellite cells and/or modulate myogenesis in vitro are: (a) the extracellular matrix protein chosen to coat the culture plates[24, 25], (b) the proteolytic enzyme(s) used to digest the muscle tissue[6], (c) the manner in which satellite cells are released from the myofibres -enzymatic digestion, mechanical dissociation (vigorous vortexing or triturations) or a combination of both[26, 27], (d) the cell seeding density[28]- and (e) the choice of growth and differentiation culture media[11, 13, 29, 30]. Taking into account all these factors, we modified Yablonka's method[21] in the following aspects: (a) the extracellular matrix protein for coating the cell culture surface was changed from gelatin to type I collagen from rat tail, (b) the collection, digestion and culture media used were modified as described in Methods, (c) for simplicity, the batch of horse serum (HS) was not pre-selected for maximal fusion, (d) muscle tissue was enzymatically digested in a water bath without shaking, (e) the use of only 10-ml pipettes to triturate the muscle tissue, and (f) instead of using Millipore Swinney filters, the digested cell suspension was conveniently filtered through a 40-μm cell strainer. We chose to employ pronase as the proteolytic enzyme because of its ability to digest the basal membrane[6]. Pronase worked well in all species tested and has been extensively used by others to isolate satellite cells from different animal species[21, 31–33].
After establishing the level of muscle satellite cells in freshly isolated cells, we performed immunocytochemistry to detect several muscle-specific muscle markers in terminally differentiated muscle cultures.
Bovine muscle cells were also readily able to proliferate and differentiate without necessitating a specific differentiation medium which was required in previous reported primary bovine muscle cultures[11, 29, 38, 39]. After 3 days of seeding in 24 well plates, bovine muscle cells underwent terminal differentiation and fusion into extensive and large multi-nucleated myotubes (Figure5e-h). Desmin expression was predominantly found in myotubes but also in a small number of myoblasts (Figure5e and f) as previously reported in bovine skeletal muscle cells[40]. Predictably, α-sarcomeric actin was restricted to myotubes only (Figure5g and h).
In our study, equine and bovine muscle satellite cells were harvested from adult animals and appeared to result in reduced yield of satellite cells in relation to the other younger animal species. This apparent reduction in yield could be related to reduced satellite cell density in muscle tissue with age as observed in some species[43–45][46]. Nevertheless, once cells reached 70% confluence, both bovine and in particular equine satellite cells, differentiated and fused rapidly to form large multi-nucleated myotubes. Equine cells fused within a short period of several hours. Whether the proliferation and/or differentiation potential of satellite cells in vitro[45, 47–49] and in vivo[50] are adversely affected by age remains to be fully resolved. Although we did not quantitatively monitor proliferation, our primary bovine and equine muscle cultures displayed an exceptional degree of myogenic differentiation, indicating that there is no need to exclusively use neonatal muscles to achieve satisfactory yield and myotube morphology in vitro.
The described modified method represents a versatile and robust way to isolate satellite cells from a variety of animal species. This relatively simple method achieves good yield of high quality satellite cells that are demonstrably able to proliferate, differentiate and fuse into extensive myotubes.
Preparation of primary muscle cultures
Skeletal muscle samples were derived from several 4- to 6-week-old Pekin ducks, from Cherry Valley Farms UK, several 4- to 6-week-old broiler chickens (ROSS 308 strain), from PD Hooks Hatcheries Thirsk UK, a 7- to 8-year-old thoroughbred horse, obtained at post mortem, several 5- 8-week-old Landrace-Large White hybrid pigs, and several 18- to 20-month-old Limousine cross heifers obtained from a local abattoir. All animals were euthanased following approved humane methods. Pectoralis major (breast) muscle from chicken and duck, biceps femoris from horse and psoas major from pig and cow were aseptically removed. Each muscle was washed once in phosphate buffered saline (PBS) and placed in collection medium, high glucose Dulbecco's Modified Eagle Media (DMEM-Glutamax I) (Invitrogen) supplemented with 1% penicillin and streptomycin (P/S) and 1% amphotericin B. All media and PBS were used at room temperature.
In the cell culture hood, each muscle tissue was washed several times in PBS, followed by the removal of associated connective tissues with a sterile scalpel before mincing with scissors. The procedure for the isolation of muscle satellite cells was modified from a previously described method as detailed by Yablonka[21] and described below.
Proteolytic digestion of muscle tissue
Minced muscle was washed once with collection medium. Approximately 4 g of minced muscle were incubated at 37 °C for 1 hour in a 50-ml conical tube in a water bath with 30 ml of dissociation medium, DMEM:Ham's F12 containing 15 mM HEPES (Invitrogen), supplemented with pronase (Sigma) at 1.4 mg/ml, 1% P/S and 1% amphotericin B. The digested muscle tissue was collected by low speed centrifugation for 6 min at 300 g and washed once in PBS (~10 ml per tube).
Isolation of muscle satellite cells
Digested muscle pellet from the previous step was resuspended in 15 ml of collection medium and subjected to vigorous triturations (repeated pipetting of tissue suspension with a 10-ml pipette for approximately 15 times). The suspension was subjected to low speed centrifugation to sediment the tissue fragments while the cells remained in suspension. The supernatant containing the released cells was collected and filtered through a 40-μm cell strainer (BD Falcon) into a new 50-ml conical tube to remove other cell debris. The flow through was then diluted in PBS (~40 ml) and centrifuged for 10 min at 800 g to pellet the cells.
Plating of muscle satellite cells
The washed cell pellet obtained in the previous step was re-suspended in appropriate growth medium and directly plated onto T75 flasks which had previously been coated with 1% type I collagen from rat tail diluted in sterile water (Sigma). All flasks were rinsed with PBS before use. Erythrocytes in the cell preparation could make an accurate cell count difficult, especially with red (oxidative) skeletal muscle. Empirically each cell pellet obtained from 4 g of minced muscle tissue would yield sufficient cells to seed onto one T75 flask. We observed a decrease in plating efficiency when more tissue was digested and plated onto one T75 as the amount of debris appeared to interfere with the attachment of cells[51]. All cells in this study were subjected to a maximum of 2 passages (trypsinization) and maintained at 37 °C in the presence of 5% CO2. For the first two days after isolation, cells were washed several times with warm PBS to remove tissue debris and erythrocytes; during this period 1% amphotericin B was also added to the growth medium. Cells were allowed to proliferate for 2 days in avian and porcine cultures and 4 days in equine and bovine cultures before trypsinization into 24-well collagen coated plates (approximately 20,000 cells per well) for immunocytochemistry. The remaining cells were frozen using 90% horse serum (HS) and 10% dimethyl sulfoxide and stored in liquid nitrogen.
Composition of Growth (GM) and differentiation medium (DM)
Avian cells were grown as well as differentiated in DMEM-Glutamax I (high glucose) with 10% HS, 4% chick embryo extract (Egg Technologies) and 1% P/S. The batch of HS was not pre-selected for maximal fusion. Equine and bovine cells were grown and differentiated in DMEM-Glutamax I (high glucose) supplemented with 20% fetal calf serum (FCS) and 1% P/S. Pig cells were grown using skeletal muscle basal medium-2 (SkBM-2) supplemented with the SkBM-2 SingleQuots kit (Lonza); when they reached 90% confluence, the GM was replaced with DM (DMEM containing 0.4% Ultroser G (Pall) and 1% P/S).
Cell fixation and immunocytochemistry
Cells were fixed at room temperature with acetone-methanol (1:1) for 10 min for immunochemical staining for muscle cell markers. Acetone:methanol solution was added at room temperature. Fixed cells were washed with Tris buffered saline (TBS) and immunochemical staining was carried out using EnVision + system-HPR (DAB) kit (DAKO) following the manufacturer's instructions. In brief, fixed cells were incubated for 10 min with an endogenous peroxidase blocker. Cells were then washed once with TBS and were incubated with a 10 μg/ml concentration of primary mouse antibody against Pax7 (R&D systems) (for avian and equine cells), or a mouse antibody against Pax7 (Developmental Studies Hybridoma Bank, kindly provided by Dr. Dylan Sweetman) used at 1:50 dilution (for porcine cells), or a rabbit anti-desmin antibody (Abcam) at 5 μg/ml or a mouse anti-α-sarcomeric actin (clone Sr-1) (Dako) at 1:50 dilution for 40 min at room temperature. Cells were rinsed four times with generous volumes of TBS and were incubated with an anti mouse or anti rabbit HRP labelled polymer for 40 min. After rinsing four times with TBS, cells were incubated with DAB substrate for 6 min, washed with TBS and counterstained with Harris' Haematoxylin. Negative controls were performed omitting the use of primary antibody.
Percentage of Pax7 positive cells in freshly isolated cells
To determine the percentage of Pax7 positive cells, immunocytochemical labeling for Pax7 was carried out. After counterstaining with Harris' Haematoxylin, 10 arbitrary fields were photographed using a x40 objective and cells presenting positive labeling were counted with respect to the total cell number. Approximately 1,000 to 1,500 total cells were counted for each species.
Fusion index
To compare the fusion rate achieved by the different species tested, fusion index was determined in differentiated muscle cell cultures. For this purpose, after either desmin or α-sarcomeric actin immunocytochemical labeling followed by counterstaining with Harris' Haematoxylin, 3 to 4 random fields were photographed using the 10-time objective lens. Nuclei within myotubes and nuclei within unfused cells were counted manually and the number of nuclei in myotubes was expressed against the total number of nuclei as a percentage. Approximately 5,000 to 7,000 cells were counted for each species. Only myotubes containing at least three nuclei were scored.
The authors gratefully acknowledge funding from the Biotechnology and Biological Sciences Research Council (BBSRC) (UK), Cherry Valley Farms (UK), Biosciences KTN (UK) and the University of Nottingham (UK). The authors also express their gratitude to C.H. Rowley Ltd. for providing cow muscle tissue and to Dr. Yablonka-Reuveni (University of Washington) (USA) for technical guidance.
Authors' Affiliations
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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


text: I was really kind of disappointed with this Coben book, his newest. The main characters were a television news reporter, five men, all room mates and graduates of Princeton University sometime in the late 1980s. All five suffer staggering defeats and obstacles in their personal lives. One of the five has been branded as a pedophile and murderer and is actually murdered himself. The television news reporter, Wendy, feels responsible for the man being classified as a pedophile and sets out to find out the truth in all the tragedies involved in the story. I thought the pace was slow but the writing was in the typical easy style of Harlan Coben. Anyone who likes his writing should, of course, still read this book.


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Spill Response is where it all started. Over 14 years of hands-on experience in facilitating tanker roll-overs, Hazardous chemical spillages and other flammable and non flammable spills. Rapid Spill Response is fully equipped to clean-up any type of spillage.
– CAIA member
– Fire Protection Association Member
– Institute of Waste Management Member


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Hi everyone. Just thought I'd pop on here to say a quick hello. I've had sick kidlets at home this week, so I haven't had much of a chance to use the computer. But I have been busy making lots of things with felt and fabric! I am still waiting on my button order to arrive so I can start making new buttons, so the shop update will most likely be next Friday.
I do have something to share with you today though. Have a look at this gorgeous layout by Stephanie Hart, featuring a cute Purple Pumpkin hedgehog button...
Doesn't that little button look so cute on her layout? To see more of Stephanie's gorgeous layouts, be sure to check out her blog HERE. (and while you're there, have a look at her shop...she makes the most AMAZING little clay embellishments!) Thanks for letting me share this Steph!
Well, I'm off to cut up some felt. Hope you are having a fabulous week!
Wednesday, August 4, 2010
A Layout To Share
Posted by Samantha Hauzer at 9:25 PM
2 comments:
- Kelly Massman said...
Very striking! I would never have thought to put those papers together but it makes for a great LO! Love your hedgie on there!
- August 5, 2010 at 3:08 AM
- Debbie said...
Really cute layout! Just got my order yesterday and can't wait to use some of my new goodies!
- August 7, 2010 at 7:35 AM
Subscribe to: Post Comments (Atom)


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By Bert Moore, Editor, AIM Global
While it is highly desirable to have a single standard for identifying animals and food products, it is not entirely necessary as long as the standard or convention being followed is understood. A company's own internal track-and-trace system, say, for fresh produce, could be used up to the point where a processor or distributor applies its own system. As long as there is a link to the producer's system, there can be an electronic ID trail back to the source.
Many food animal producers have been using bar code or RFID ear, collar or leg tags for years for internal processes. Extending the use of the IDs on these tags throughout the food animal production system (ranch, transport, feed lot, auction house, processor) would not be as monumental or costly as might initially be assumed.
Some large agricultural companies have already embraced the idea of field-to-fork (or at least field-to-processing plant) traceability to limit the size of product recalls, protect brand image and, incidentally, safeguard the public. Many of these companies own or control the production or output in other countries and could, therefore, adopt a track-and-trace methodology for their local businesses without the need for local government regulation or cooperation.


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Parker Robinson Pvt Ltd is a cGMP certified Company with complaint revised schedule "M" unit and also have a DSIR (Department of Scientific and Industrial Research, Government of India, New Delhi) recognized in house R&D unit.
Our major thrust areas are towards total eradication of 'Broad Spectrum Derma Diseases' for which we manufacture antifungal, antibacterial, antiscabies, keratolytic, antibiotic creams and lotions with effective dosage to give better treatment to the patient, in the field of derma diseases. Besides that we have also a wide range of corticosteroids with antifungal & antibacterial combinations to enhance our service to mankind to combat major derma diseases. Anti Psoriasis Drugs of School of Tropical Medicine Research Product was launched by us and we are manufacturing regularly with proven efficacy. Antibiotic, gentamicin sulphate is being manufactured in various dosages forms and gentamicin tropical lotion which is our one of the prestigious product is unique in its activity and almost zero skin toxicity.
External application requires a good penetration enhancer for better absorption of the active ingredients and ultimately it is phased out causing no skin toxicity. Cream based on urea is much helpful for Its manifold action and one such product is Urea - 10 skin cream (IP, BP) which widely used during winter season as a moisturizer along with Urea-E cream which is a combination product of Urea & Vitamin E.
We are always aiming to bring new formulation drugs to extend our fruitful service for the present and upcoming generations and to save them from Critical Derma Diseases because due to climatic change, global warming & atmospheric pollution new diseases are prone to develop in tropical countries like ours, which needs much care & research to find out proper solution and treat them scientifically.
Besides that recently our company pursuing the scientific researches on a wide range of herbal products from different natural resources to enhance our service to mankind to combat against major derma and other broad spectrum diseases. Research products have been launched by us after successful trial and one such product is Alovee gel (20% aloe vera) which widely used during winter season as a moisturizer along with Alovee-E cream which is a combination product of Aloe vera (10% aloe vera) & Vitamin E. Other prestigious products in our herbal field are an anti-inflammatory preparation namely Aloebalm and anti-flatulent and digestive preparation namely Aquajwain (Jowan Ka Ark).


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On this week's show we chat about games coming and going from the free-to-play world, really dig into some EverQuest news, speculate on Steam's Review Bombs fix, and chart out our Guid Wars 2 PoF plans. All that and more on this week's Free to Play Cast!
Discussion Timestamps:
01:36 Free-to-Play Games Coming and Going
40:26 Valve Tries to Fix Review Bombing
48:18 Guild Wars 2 Path of Fire Plans
50:01 Weekly Bombs (DA-Bomb / A-Bomb)
58:15 Questions and Answers of the Week
If you have submissions for the show's Weekly Bombs, have questions for the panel (nothing is off limits!), or just ideas for what you want covered you can now send them to firstname.lastname@example.com. You can also be a guest in the show, just send us why you want to host, and what topic or game you would like to cover.
Free-To-Play Cast is the official podcast (and videocast) of MMOBomb.com about free to play multiplayer online games. Free-To-Play Cast takes on the week's biggest news with plenty of opinion, weekly bombs, community feedback, guests, laughs thrown in for good measure and much more. Have fun and expect a new episode next week!
3 Readers CommentedJoin discussion
For the record: I'm LOVING Fortnite and just hope they add things like winning tokens and unlockables etc….but it is VERY EARLY and yet it is VERY FUN and that's the only thing that matters to me! <3
Fortnite Battle Royale that is lol
Da-Bomb to you guys! Opposite to the commenter last week, your format is a big reason I watch (or listen if I'm farming). I like seeing the varying opinions, one of which usually aligns with mine, and you guys just have fun chemistry in your communication together. So I say keep it up.


text: Did you get a phone call or text message from (859)410-6201? Other formats of number (859)410-6201 are 8594106201 and 859-410-6201, area code is 859, in Lexington, KY. The phone number (859)410-6201 is used for telemarketing, scamming and silent or blank call. The caller does not make any response if you call back to the number. The phone number used for the bad purpose. So, you should avoid the call if you get any missed call from the phone number. You may get different offers from the person and you must not the response of the call. If you get any offer from the person, you should avoid those. The caller of the phone number can give miss call and call but he or she does not leave any massage. You should avoid the call and do not response. ,


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"St. Petersburg Open" - one of the major sports events in St. Petersburg
The history of "St. Petersburg Open" has started in 1995. That year the first international ATP Tour tennis tournament took place in St. Petersburg. The city received the right of holding the Tournament after the victory in the ATP Tour contest over Budapest (Hungary) and Graz (Austria).
The Tournament immediately became the important event in the life of the city and the whole country as well. The participants list of the new Tournament was really impressive: Yevgeny Kafelnikov, No. 10 in the ATP rating of that period, Thomas Muster from Austria - outstanding player of the season 1995. The Tournament results inspired both - organizers and spectators: three Russians - Kafelnikov, Chesnokov and Volkov have reached the semifinals. The first winner of "St. Petersburg Open" was the best player of the last century.
From the very first Tournament the organizers paid great attention towards the social program of the event. Sightseeing tours to the Hermitage and the Russian Museum, players' parties, Pro-Am tournaments have always been the essential part of the Tournament. Participants of the first tournaments still remember helicopter flights over the Ladoga lake and winter fishing:
A year later Kafelnikov was again at the top of the participants list and had a chance to repeat his result. That time he had reached the final in the singles draw and won the pairs competition together with Andrey Olkhovsky. Magnus Gustafsson from Sweden became the winner of the second "St. Petersburg Open" and another famous player from Scandinavia - Thomas Johansson won the tournament of 1997.
"St. Petersburg Open" had a difficult times those years and only the support of the city Administration (the Governor of St. Petersburg Vladimir Yakovlev is traditionally the Chaiman of the Organizing Committee) and the commercial companies have helped it to overcome the difficulties. Even in these hard conditions the list of participants has always been impressive. The Tournament 1998 final was played by Wimbledon 1996 winner Richard Krajicek and the Russian fans favorite Mark Rosset from Switzerland. That time Rosset was defeated by the Dutch player, but a year later he won this title in the match against the German player David Prinosil.
The year 1999 became the crucial moment in the history of "St. Petersburg Open". One of the major banks of the North-Western region has purchased the rights for the Tournament from its first owner - Professional Tennis League. The Tournament financial situation improved significantly and it allowed to draw even more attention of the fans towards the Tournament. Due to these in 1999 "St. Petersburg Open" has gained the ATP award for the Record Attendance.
Furthermore the prize money was increased from 340,000 to 800,000 dollars(2000 - 2001), and in the year 2002 increased again to $1,000,000, that fact rose "St. Petersburg Open" to the level of "Kremlin Cup". The Tournament winner now receives 50 points in the ATP rating - maximum for the Tournament of this level.
The dates of holding the Tournament have been also changed - from February, the start of the season, to autumn - closer to its end. New position in the ATP calendar (right before the World Championship) makes the Tournament more interesting for the world top players - the chance to gain necessary points for getting into the World Eight in the Masters Cup.
Due to this the participants list has changed immediately - any ATP Champions Series Tournament could envy "St. Petersburg Open-2000": for the first time two of the Top six players have played on the courts of the Peterburgsky Sports Complex. They were the US Open winner Marat Safin and Olympic Champion Yevgeny Kafelnikov. Marat Safin won the "St. Petersburg Open" title in 2000.
The development in the technical aspect was noticeable as well. The most up-to-date surface - GreenSet Grand Prix - was laid in the Sports Complex. It is traditionally used in the prestigious indoor tournaments. The players (Marat Safin, Dominic Hrbaty, Jonas Bjorkman and others) highly estimated the new courts.
"St. Petersburg Open", which is traditionally held in one of the largest European indoor arenas, provides a unique environment for relationship building for companies interested in exploring or enhancing their positions in the Russian marketplace. Sports Complex Peterburgsky can seat up to 25,000 spectators (the capacity of the Central Court tribunes is 11,000 seats). The number of spectators annually attending "St. Petersburg Open" is more than 40,000 (last year - 42,000). Thus sponsors and partners of "St. Petersburg Open" gain incredible advertising opportunities and benefit from being associated with this top class event.
Tournament 2002 | Tournament history | Sponsors & partners
News | Tickets | Tennis tours | Contest
Contacts | SCC | Statistics | Search | E-mail


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Lumberjaxe is opening up a permanent spot in Fall 2017.
Throwing darts is so last year. At least, that's what the owners of Lumberjaxe Charlotte will tell you. This new business is all about throwing axes at wooden targets, and it couldn't be more exciting.
While the company doesn't have a permanent location yet, they plan to open a facility by Fall 2017. Until then, they're utilizing an old truck as a pop-up ax-throwing facility – complete with instructions.
Look for where they'll be next on their Facebook page.


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2 Invitations & Stationery in Donegal meet your criteria
Invitations & Stationery in Donegal
10 Sheares Street, Cork City
TypeInvitations & Stationery
We are specialists in designing and producing tailor made wedding stationery. We will do everything to assist in making the process as easy as possible. We cover all areas of your Weddings; from your engagement party, right up to after your special day.
Last Update on 21st Jan 2016
Kerry Harvey
Clar Road, Donegal Town, Donegal
TypeInvitations & Stationery
Trend-setting, award winning, beautifully designed wedding stationery collections for all tastes! Stand out from the crowd with our vintage chic, to quirky character designs, with fresh new collections to chose from every season!
Last Update on 14th Aug 2015


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The Nebraska baseball team (6-13, 0-0 Big Ten) took a 4-2 lead into the bottom of the sixth inning on Wednesday night at Goodwin Field, only to see 8th ranked Cal State Fullerton rally for eight runs on their way to a 10-4 win and two-game series sweep against the Huskers. The Huskers controlled the first half of the game, holding the Titans to two runs on three hits. The NU offense got a lift from Chad Christensen, who hit his second home run of the season and Austin Darby added a triple. NU also got doubles from both Kash Kalkowski and Wes Edrington.
Fullerton took over the game with eight runs on six hits and three hit batters in the sixth to take a 10-4 lead. The Titans scored five runs on three straight one-out singles, followed later by a two-out RBI double, an RBI triple and then a wild pitch.
Nebraska will catch a flight tomorrow morning to Illinois, where it will open Big Ten play on Friday against the Illinois Fighting Illini at 4:05 p.m. The teams are also scheduled to meet on Saturday at 3:05 p.m. and Sunday at 12:05 p.m.


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I can almost hear the crunchy sound of biting into one of these maltesers just by looking at them. At my work we have a cake giving occasion anytime someone experiences something new or big in their private lives. So a colleague of mine had promised to give the others cake because it was his birthday. But he was like we always have the same type of cake so I asked if I should make some cupcakes in his name? Well he was thrilled and I made these also Double Chocolate with maltesers which received a lot of praise :-).
I used my favourite double chocolate decadence recipe and topped it off with nougat-buttercream frosting and a piece of malteser on top of each.
For the frosting:
*150g soft nougat
*250g unsalted butter
*350g icing sugar
*2 tbs bitter sweet cocoa powder
*1 tbs milk or heavy cream
*maltesers for…
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