text:
- Research
- Open Access
Prevalence of resistance associated polymorphisms in Plasmodium falciparum field isolates from southern Pakistan
- Najia Karim Ghanchi†1,
- Johan Ursing†2Email author,
- Mohammad A Beg1,
- Maria I Veiga2, 3,
- Sana Jafri1 and
- Andreas Mårtensson2, 4
© Ghanchi et al; licensee BioMed Central Ltd. 2011
- Received: 21 October 2010
- Accepted: 28 January 2011
- Published: 28 January 2011
Scarce data are available on Plasmodium falciparum anti-malarial drug resistance in Pakistan. The aim of this study was, therefore, to determine the prevalence of P. falciparum resistance associated polymorphisms in field isolates from southern Pakistan.
Blood samples from 244 patients with blood-slide confirmed P. falciparum mono-infections were collected between 2005-2007. Single nucleotide polymorphisms in the P. falciparum chloroquine resistance transporter (pfcrt K76T), multi drug resistance (pfmdr1 N86Y), dihydrofolate reductase (pfdhfr A16V, N51I, C59R, S108N, I164L) and dihydropteroate synthetase (pfdhps A436S, G437A and E540K) genes and pfmdr1 gene copy numbers were determined using PCR based methods.
The prevalence of pfcrt 76T and pfmdr1 86Y was 93% and 57%, respectively. The prevalence of pfdhfr double mutations 59R + 108N/51R + 108N was 92%. The pfdhfr triple mutation (51I, 59R, 108N) occurred in 3% of samples. The pfdhfr (51I, 59R, 108N) and pfdhps (437G, 540E) quintuple mutation was found in one isolate. Pfdhps 437G was observed in 51% and 540E in 1% of the isolates. One isolate had two pfmdr1 copies and carried the pfmdr1 86Y and pfcrt 76T alleles.
The results indicate high prevalence of in vivo resistance to chloroquine, whereas high grade resistance to sulphadoxine-pyrimethamine does not appear to be widespread among P. falciparum in southern Pakistan.
- Artesunate
- Chloroquine Resistance
- Lumefantrine
- Pfmdr1 Gene
- Pfmdr1 Copy Number
Development and spread of Plasmodium falciparum resistance to anti-malarial drugs represents a major threat to global malaria control. In Pakistan, an estimated 500,000 episodes of malaria infection occur annually [1]. The incidence of malaria has markedly increased during the last decade and the relative frequency of P. falciparum amongst blood-slide positive malaria infections has increased from 45% in 1995 to 68% in 2006 [2–4], probably due to increasing resistance to commonly used monotherapies.
Chloroquine resistance was first reported from Pakistan in 1984 and was followed by several reports confirming it in Punjab, Afghan refugee camps and areas bordering Afghanistan [5–8]. In agreement with recommendations by the World Health Organization, Pakistan has adopted artemisinin-based combination therapy (ACT) as treatment of choice for uncomplicated P. falciparum malaria with artesunate plus sulphadoxine-pyrimethamine as first-line treatment [9]. However, the use of this combination is of some concern as resistance to sulphadoxine-pyrimethamine monotherapy has been reported from western Pakistan [10].
Analyses of molecular markers associated with P. falciparum anti-malarial drug resistance can provide important information about levels of sulphadoxine-pyrimethamine resistance. Single nucleotide polymorphisms (SNPs) at codons 51, 59, 108 and 164 in the P. falciparum dihydrofolate reductase gene (pfdhfr) are well established determinants of pyrimethamine resistance [11]. An initial mutation at codon 108 causes 7-50 fold increase in the in vitro inhibitory concentration (IC50). The presence of additional mutations further increase IC50 and the triple mutant (N51I/C59R/S108N) is associated with clinical treatment failure [12, 13]. Similarly, mutations at codons 436, 437, 540, 581 and 613 in the P. falciparum dihydroptereoate synthase gene (pfdhps) are associated with sulphadoxine resistance [14, 15].
Other genetic polymorphisms have also been associated with P. falciparum drug tolerance/resistance. The K76T amino acid substitution in the chloroquine resistance transporter gene (pfcrt) has been shown to be essential for chloroquine resistance, associated with amodiaquine resistance and predictive of treatment failure for both drugs [16, 17]. Pfcrt 76K has been associated with lumefantrine tolerance/resistance and higher IC50 values [18–20]. The P. falciparum multidrug resistance gene 1 ( pfmdr1) allele 86Y has been associated with chloroquine and amodiaquine resistance and increased chloroquine IC50 values in P. falciparum with pfcrt 76T [21, 22]. In a meta analysis pfmdr1 86Y was significantly linked to chloroquine and amodiaquine resistance [23]. Conversely, pfmdr1 86N has been associated with lumefantrine tolerance/resistance and higher lumefantrine IC50 [20, 24, 25]. Pfmdr1 amplifications are associated with mefloquine resistance in vivo and in vitro, doubled lumefantrine IC50 and reduced sensitivity to artesunate [26].
Scarce data are available on anti-malarial drug resistance among the P. falciparum population in Pakistan. The aim of this study was, therefore, to assess the prevalence of P. falciparum resistance associated polymorphisms in field isolates from southern Pakistan.
Study setting, participants and ethics
The study was conducted between October 2005 and October 2007 at the Aga Khan University Hospital, a tertiary hospital located in central Karachi, and its established chain of primary health care and diagnostic service centres located in Sindh and Baluchistan provinces, Pakistan. In the study area, malaria transmission peaks during and after the monsoon season that lasts from June to October. Patients with microscopy confirmed P. falciparum mono-infection were eligible for enrolment irrespective of age, sex and disease severity.
The study was conducted in accordance with the Declaration of Helsinki and Good Clinical Practice [27]. Informed consent was obtained from all participants or in case of children from their parents/legal guardians. The study was approved by the ethical review committee of Aga Khan University Hospital, Karachi, Pakistan.
Blood collection and microscopy
Two ml of intravenous blood were collected in an EDTA tube from all patients referred to the laboratory for investigation of malaria infection. For screening purposes a thick blood film was prepared and analysed using Leishman stain according to routine practice. In case of a positive screening result, a thick and thin Giemsa-stained blood film was prepared for confirmation of the presence of malaria parasites and species identification. For all patients with confirmed P. falciparum mono-infection the parasite density was assessed by counting asexual parasites against 200 white blood cells (WBC) on the thick film and quantified (parasites/μl) by assuming an average of 8000 WBC per μl blood [28]. All blood slides were examined by experienced microscopists at the clinical laboratory of Aga Khan University Hospital. For quality control, 10% of the blood slides were re-examined by an independent microscopist unaware of the initial result.
From each sample 100 μl of blood was spotted on an FTA® filter paper (Whatman), dried, then put in a separate plastic bag and stored at -80°C. The remaining blood was transferred to cryo-vials and kept frozen at -80ºC until used for DNA extraction. A brief epidemiological and demographic history was also collected from each participant using a structured questionnaire.
DNA extraction
DNA was extracted using Qiamp DNA mini Kits (Qiagen, USA) from 200 μl of whole blood at Aga Khan University Hospital. The FTA® filter papers were transported to Karolinska Institutet, where DNA was extracted from approximately half of each blood sample (50 μl) using an ABI Prism® 6100 Nucleic Acid Prep Station (Applied Biosystems, Fresno, CA). In both cases DNA extraction was according to the manufacturer's instructions. Extracted DNA was stored at -20°C until amplified by PCR.
Pfcrt K76T and pfmdr1 N86Y alleles were analysed for all samples at Aga Khan University Hospital. Quality control was done at Karolinska Institutet by genotyping a randomly selected subset of 25% of the samples. The same multiplex PCR-RFLP protocol [29] was used at both laboratories.
SNPs at pfdhfr codons 16, 51, 59, 108 and 164, and pfdhps codons 436, 437 and 540 were identified by sequencing according to a previously described method [30]. The PCRs were performed at Karolinska Institutet. The products were purified and sequenced commercially (Macrogen Inc. Seoul, Korea). For quality control, the genotype at codon 540 was determined from 25% of the samples using a PCR-RFLP based method [29]. Codon 540 was chosen because of its location towards the end of the amplified segment, where sequencing is less robust. PCR and restriction products were resolved on 2% agarose gels (Amresco, Solon, OH). All gels were stained with ethidium bromide and visualized under UV transillumination (GelDoc®, Biorad, Hercules, Ca, USA).
Pfmdr1 gene copy number variation polymorphism was determined using real time PCR (ABI Prism® 7000 Sequence Detection System) as previously described [26]. All samples were run in triplicate. The clones, 3D7, D10 and K1 were used as single copy calibrators and FCB and Dd2 represented multiple copy controls. Pfmdr1 copy numbers were calculated using a comparative threshold method (ΔΔCt method) [26]. An amplification of pfmdr1 was defined as copy number >1.6. Assays were repeated if the following results were obtained: copy number 1.3-1.6 or Ct value >35 or ΔΔCt spread >1.5.
Power calculation and statistical analyses
This was a descriptive/exploratory study precluding a power calculation of sample size. Data were entered, validated and analysed using SPSS version 16.0. The Sequencher ™ software version 4.6 (Gene Codes Corporation, Ann Arbor, MI) was used to analyse the sequences output using the 3D7 clone sequence obtained from NCBI database (pfdhfr Accession # JO4643 and pfdhps XM_001349382) as a reference. Allele proportions were calculated as the number carrying a certain allele divided by the number of samples with positive PCR outcome. Mixed infections thus contribute to the prevalence of both alleles.
Baseline characteristics of enrolled patients.
(n = 244)
(n = 182)
(n = 52)
(n = 10)
Age *
≤5 years
35 (0.14)
16 (0.09)
17 (0.33)
2 (0.20)
6-15 years
39 (0.16)
26 (0.14)
12 (0.23)
1 (0.10)
>15 years
170 (0.70)
140 (0.77)
23 (0.44)
7 (0.70)
173 (0.71)
137 (0.75)
31 (0.60)
5 (0.50)
71 (0.29)
45 (0.25)
21 (0.40)
5 (0.50)
Parasite density § (parasites/μl)
11100 (80-540000)
12720 (80-540000)
7480 (80-126000)
66160 (240-230220)
Gametocyte carriage
96 (0.45)
69 (0.43)
23 (0.48)
4 (0.45)
Pfcrt and pfmdr1 SNPs
Prevalence of pfcrt 76K, 76T, pfmdr1 86N and 86Y alleles in southern Pakistan
Pfmdr 1
16 (0.09)
161 (0.91)
1 (0.006)
73 (0.41)
84 (0.47)
21 (0.12)
52 (1)
25 (0.48)
20 (0.39)
7 (0.14)
1 (0.10)
9 (0.90)
6 (0.60)
4 (0.40)
17 (0.07)
222 (0.93)
1 (0.004)
104 (0.43)
108 (0.45)
28 (0.12)
Pfmdr1 copy number
The real-time PCR was successful in 232/240 (97%) samples. A single pfmdr1 gene was found in 231 samples. One sample had 2 copies of the pfmdr1 gene and carried the pfmdr1 86Y and pfcrt 76T alleles.
Pfdhfr and pfdhps
Prevalence of resistance associated single nucleotide polymorphisms in pfdhfr and pfdhps from southern Pakistan
16 (0.1)
144 (0.89)
159 (0.99)
1 (0.006)
99 (0.58)
3 (0.02)
2 (0.04)
44 (0.92)
47 (0.98)
13 (0.26)
9 (1)
9 (1)
6 (0.60)
18 (0.08)*
197 (0.90)§
215 (0.99)
1 (0.004)
118 (0.51)
3 (0.01)†
Prevalence of pfdhfr- pfdhps haplotypes in P. falciparum isolates from southern Pakistan.
Pfdhfr N51I, C59R, S108N, I164L
Pfdhps S436A, A437G, K540E*
No. of isolates
No. of
(n = 158)
(n = 47)
(n = 9)
(n = 214)
1 (0.02)
1 (0.005)
80 (0.51)
9 (0.19)
5 (0.55)
94 (0.44)
55 (0.35)
33 (0.70)
4 (0.45)
92 (0.43)
1 (0.006)
1 (0.005)
1 (0.006)
1 (0.005)
4 (0.03)
1 (0.02)
5 (0.02)
5 (0.03)
5 (0.02)
3 (0.02)
1 (0.02)
4 (0.02)
2 (0.01)
2 (0.01)
3 (0.02)
3 (0.01)
2 (0.01)
2 (0.04)
4 (0.02)
1 (0.006)
1 (0.006)
This is the most comprehensive report characterizing resistance associated genetic polymorphisms in P. falciparum field samples collected in southern Pakistan. As such, the results bridge an important knowledge gap of the P. falciparum population in South Asia.
The 93% prevalence of pfcrt 76T, essential for chloroquine resistance, is in line with results from neighbouring countries [16, 31, 32]. This data indicate high levels of in vivo P. falciparum chloroquine resistance in southern Pakistan. Moreover, the high pfcrt 76T and moderately high (57%) pfmdr1 86Y prevalence also suggests high levels of tolerance/resistance to amodiaquine in the study area.
The high prevalence of the pfdhfr 108N (99%) and 51I + 108N/59R + 108N (92%) in our study indicate that decreased susceptibility to sulphadoxine-pyrimethamine is widespread in Pakistan. However, only seven patients had infections with the triple pfdhfr resistance associated haplotype and only one patient was infected with P. falciparum that had the quintuple pfdhfr + pfdhps haplotype associated with high grade sulphadoxine-pyrimethamine resistance. These results indicate that high grade resistance to sulphadoxine-pyrimethamine is not widespread and suggest that this drug is probably suitable for use with artesunate in southern Pakistan, as recommended by the National Malaria Control Programme. However, the occurrence of triple and quintuple mutant P. falciparum is of concern as widespread use of sulphadoxine-pyrimethamine as a partner to artesunate may rapidly select these haplotypes. Monitoring of pfdhfr and pfdhps resistance associated haplotypes is consequently of importance. Furthermore, artesunate + sulphadoxine-pyrimethamine in vivo efficacy urgently needs to be assessed. This is critical as efficacy studies conducted in Baluchistan (2001-2005) reported 56% treatment failure with sulphadoxine-pyrimethamine monotherapy [33]
Only one patient had a P. falciparum infection with two copies of pfmdr1, a finding that should be interpreted with caution. Increased pfmdr1 copy number has been associated with an increased risk for treatment failure after mefloquine monotherapy and artesunate-mefloquine therapy [26]. The low prevalence of pfmdr1 amplifications observed in this study suggests that both artesunate-mefloquine and artemether-lumefantrine would be efficacious in southern Pakistan. They may therefore represent potential future treatment alternatives to artesunate + sulphadoxine-pyrimethamine. Furthermore, the observed low prevalence of pfcrt 76K and pfmdr1 86N provides supporting evidence of a probable high artemether-lumefantrine efficacy [20, 24]
This data concur with recent results from a small study (n = 28) conducted in Khyber Pkhtunkhwan Province, Pakistan and previous data from India and Iran [34]. Furthermore, just as in northern India and Iran the pfdhfr A16N51R59N108I164 and A16I 5 1R59N108I164 were the most common double and triple mutants found [35, 36]. These results thus support a selective sweep of chloroquine and sulphadoxine-pyrimethamine resistant P. falciparum from southeast Asia via India to Pakistan and then on to Iran [37–40]
It should be underlined that a majority of patients (74%) presented with symptomatic malaria infection at the Aga Khan University Hospital that represents tertiary level of care. Some of these patients may have received anti-malarial treatment prior to enrolment in this study and the P. falciparum SNP proportions reported may, therefore, not be representative for the parasite population in southern Pakistan. However, the similarity of our results with those reported from neighbouring countries suggests that the results may be generalized.
The results indicate high prevalence of in vivo resistance to chloroquine among P. falciparum in southern Pakistan, but high grade resistance to sulfadoxine-pyrimethamine does not appear to be widespread in the parasite population. This data thus support the recent change of first line therapy for uncomplicated P. falciparum malaria from chloroquine to artesunate + sulphadoxine-pyrimethamine. Continued anti-malarial drug resistance surveillance in Pakistan is essential.
This work was supported by University Research Council grant (URC # 051012 P&M) by Aga Khan University (MAB). Higher Education Commission, Government of Pakistan and The Swedish institute (NKG) (Ref # 042-130358-Ls2305). The Swedish South Asian Studies Network 2005 and 2008 (AM; JU). Fundacão para a Ciência e Tecnologia, Portugal, PhD Grant (SFRH/BD/28393/2006) (MIV). We gratefully acknowledge Clinical Laboratory staff at Aga Khan University Hospital for providing us malaria positive samples. We also thank colleagues at the Malaria Research Laboratory, Karolinska Institutet, for support and valuable comments, and Dr. Sabina Dahlström for molecular training (NKG).
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It's Day 2 of the Pokemon Challenge!
Least Favorite PokemonAnime-wise, it's definitely
The anime creators do an amazing job of making him truly annoying. I really do feel like Misty sometimes with how frustrating this duck is. I love ducks. Farfetch'd is one of my favorites. But omg Psyduck just gets on my nerves.
In the games, I'd have to say
Especially if you're fighting him with Prof. Kukui as his trainer. I never had to use so many potions in one fight. At one point in my battle, it was just each of us healing our pokemon non-stop!
Who is your least favorite Pokemon?
That's all for now! See you next time!

subset: commoncrawl


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Wordsworth Street.
Photograph © Mairead Hardy 2015
Friday, 17 July 2015
Pots on steps
1 comment:
- Gunn said...
Looks pretty!
I do like all the different colourful doors you have in England.
And the gardens, even the small front gardens, many filled with beautiful roses.
Greetings from Gunn / Stavanger / Norway.
- 18 July 2015 at 19:07
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Ohio Insurance Salesman Indicted for Impairing and Impeding the IRS
William R. Herder of Bellville, Ohio, was arrested today on federal tax charges, Assistant Attorney General of the Justice Department's Tax Division Kathryn Keneally, U.S. Attorney for the Northern District of Ohio Stephen M. Dettelbach and Special Agent in Charge, Internal Revenue Service (IRS) Criminal Investigation, Cincinnati Field Office, Darryl K. Williams announced. On June 19, 2012, a federal grand jury sitting in Cleveland returned an indictment against Herder, charging him with corruptly endeavoring to impair and impede the due administration of the Internal Revenue laws, failure to file tax returns, failure to pay taxes, and structuring transactions to evade currency reporting requirements.
According to the indictment, Herder, an insurance salesman, failed to file timely and accurate income tax returns for the years 2000-2009 despite earning substantial insurance commissions and receiving warnings and notices from the IRS. Herder filed returns for the years 2010 and 2011 on which he reported that he owed taxes to the government, but failed to pay the almost $50,000 in taxes that he owed for those years.
The indictment further alleges that, to prevent the IRS from collecting his unpaid taxes, Herder attempted to conceal his assets and income. In 2004, Herder formed two entities in Nevada--one for the purpose of hiding his automobiles and another for the purpose of hiding his insurance business. Herder also began converting his insurance commission checks to cash and paying his expenses in cash to prevent the IRS from collecting his taxes from his bank account.
In addition to failing to file valid tax returns and hiding his assets from the IRS, the indictment alleges that Herder submitted numerous obstructive letters and documents to the IRS and the company for whom he sold insurance in an effort to prevent the IRS from assessing and collecting his taxes. In these letters, Herder falsely claimed, among other things, that the tax laws were not applicable to him. In 2005, Herder also attempted to pay his taxes with a fake financial instrument called an "International Bill of Exchange."
The case was investigated by special agents of IRS – Criminal Investigation and is being prosecuted by Tax Division Trial Attorneys Melissa S. Siskind and Jeffrey A. McLellan.


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SMDH Bill O'Reilly Stays Saying Some Racist Ish: Nine Year Old Children In The Ghetto Smoke Pot Because It's Their Culture!
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Arsonist strikes Chinese consulate in San Francisco
The Chinese consulate in San Francisco sustained fire and smoke damage after an arsonist struck it, the consulate said on Thursday.
The Chinese consulate in San Francisco sustained fire and smoke damage after an arsonist struck it, the consulate said on Thursday, but there were no injuries and the consulate was open for business.
The consulate said video cameras recorded the arsonist on Wednesday night, carrying two cans of gasoline from a van parked on the street and then setting an embassy gate ablaze. The San Francisco Fire Department said it responded two minutes after the fire was reported and quickly put out the fire. The U.S. FBI, which is investigating the arson, said no suspects have been arrested.
"We strongly condemn this despicable attack," said Wang Chuan, the consulate spokesman, reading from a statement at a brief news conference. "We urge the U.S. side to take all necessary measures to provide adequate protection for Chinese consular personnel and properties."
Chuan gave the news conference standing in front of the Laguna Street entrance to the consulate, where a door flanked by statues of lions was charred and the area above it blackened by smoke. Broken glass from a window above the gateway of the door lay on the steps, along with some debris. The interior door just inside the gate was boarded with plywood.
The entrance hit by the arsonist is around the corner from where most of the public enters the building on Geary Street.
The consulate said it was working with U.S. authorities, including the San Francisco Police Department, the Fire Department and the Bureau of Diplomatic Security of the U.S. State Department, and urged them to solve the crime quickly.
The San Francisco Fire Department said it received a report of a fire at 9:33 p.m. PST, arrived on the scene within two minutes and brought the fire under control within six minutes, and has turned over the investigation to the Federal Bureau of Investigation.
FBI spokesman Peter Lee said the FBI's evidence response team responded to the scene on Wednesday night "to see if there is any actionable evidence to hopefully bring the perpetrator or perpetrators to justice."
A small group of staff members was in the consulate when it was attacked, Chuan said. He said video cameras recorded the suspect throwing at least one bottle through the glass over the gate. He declined to provide details on the suspect's gender, age or ethnicity, or to speculate about possible motives.
The consulate will remain open for business, he added. Dozens of visa applicants entered the consulate after lining up early at the Geary Street entrance, some unaware of the fire that had burned around the corner.
State Department spokeswoman Marie Harf said the United States is "deeply concerned" by the reported arson attack. "We take this incident very seriously," she said at a briefing in Washington. She did not comment on whether security was being stepped up at the consulate or at the Chinese Embassy in Washington, and said she was not aware of any specific threats against the consulate.
Protests are common outside Chinese diplomatic missions in Western countries, but acts of violence are rare.
The safety of diplomatic personnel and missions is a sensitive issue in China after a 1999 incident in which the U.S. military accidentally bombed the Chinese embassy in Belgrade during air strikes against Serbia.
That bombing, which many Chinese still believe was intentional, sparked violent anti-NATO demonstrations across China and brought relations with the United States close to a breaking point.


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I was all kinds of famous today and I totally forgot to mention it. What can I say? I can't keep up with things here. Life is really kicking my butt lately!
Anyway, you can catch more of my witty, pithy storytelling at Coloring Outside The Line. The Crayon Wrangler asked ME to guest blog for her this week, and after I picked up my jaw off the floor and verified that she was neither drinking nor on a controlled substance, I was happy to oblige. Check out my post by clicking HERE!


text: Moving Picture Company India (MPC) has partnered with Movico Technologies to digitize, promote and sell archival footage through Movico's new video aggregation website.MPC owns approximately 12,000 hours of video footage that include travel destinations, exotic locations, political events, profiles of Bollywood stars, celebrities, music performances and stock footage from India, Afghanistan and Bhutan. Movico will undertake the digitization, classification and repurposing of footage that will allow Moving Picture Company to easily access the video library for its own production use. It will also help the Company make select footage, available for worldwide sale, to other content producers.Movico Technologies is a video software startup that integrates key video, mobile, PC and internet technologies to build next generation video software products to serve the needs of video producers and distributors.Said Movico Technologies director and co-founder V N Saroja, "The deal has been divided into two segments. In the first segment, we will digitize and catalogue MPC footage, identify the best pieces and repurpose the same for sale. For providing this service, we will receive payment on cost per hour basis. The second phase includes marketing of the repurposed footage, which will be available on our website. The revenue generated will then be shared between us and MPC," she added.Adding further, MPC chairman and MD Ramesh Sharma said, "We have always believed our library has great value waiting to be unlocked, and this partnership with Movico will allow us to tap both the Indian and the global market."Also, Movico has tied up with several international stock footage websites for cross-promotions. Besides, it has tied-up with international producers for stock footage consumption and with content distribution platforms for global delivery.Movico has plans to create a sophisticated, tightly integrated, multi-purpose video production, storage and broadcast management system capable of handling enterprise class loads. Hence, to materialize the same, the company is aiming to invest $1 million in the project. Additionally, the company also intends to raise $5 million this year to strengthen its portfolio of product, service, and aggregation, targeted at the video content market.


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We happened upon Fresh to Frozen just a few weeks after it opened at its first location and always look forward to stopping in as often as possible. We loooooooove Fresh to Frozen and cannot sing its praises loud or long enough. We have saved TONS there. We also shopped there Saturday and one of the most awesome deals we found was a monstrous bag of gourmet salad greens for $5. To this we added a $2 bag of 'gourmet' Stir Fry blend ($2) that included broccoli, snap peas, carrot shreds, etc….I say 'gourmet' because the veg in this blend tasted so fresh and was so tender. We have a big family and made salad our Sunday dinner and had lots left over. We also picked up a box of Clementines (price escapes me) they were sampling at the door. Great deal. Chocolate Silk milk for 50 cents per carton. (Last time it was Organic chocolate soy milk marked down to 50 cents per carton.) We've been picking up nitrite free Oscar Mayer hot dogs for $1 each time we've been the last few months. (These hot dogs are awesome!) When things like that get close to their sell by date, they'll sometimes mark them down super low (like the Oscar Mayer hot dogs we picked up for 25 cents each a few years ago) or they'll freeze them like these nitrite free ones. There were also 30-count large flour tortillas that were incredibly soft and were selling for $1.50!
The loaves of Nature's Own bread for $1.19 that I've picked up have never been stale, and $1.19 is awesome. I have bought pounds and pounds of bananas for 10 cents/lb that made the best banana bread or banana smoothies I've ever had. We have often gotten cases of Breyers and sometimes organic yogurt there for $2. That's $2 for 24 full size yogurt cups!
No, not everything in there is a steal. I don't often buy cold cereal there and don't buy jarred spaghetti sauce anyway. We all know our price points, so I stick with those at this store, too.
Our deals there are endless, and I could go on. I've often gone in to find organic cereal or frozen pizza for pennies on the dollar of what I would find it at say Good Foods Grocery (and I don't buy those either, but I always feel sorry for the poor souls who are at that very moment paying full price at GFG for it!).
If you're used to paying full retail and getting bargains with coupons on name brand items and enjoy the convenience (not checking for exp dates, spoilage, etc.) of that, then you may not love a salvage grocery quite as much as I do. I do that, too, like the $171 worth of groceries we picked up at Kroger Saturday night for $35 after subtracting the catalinas. But if you're in for the thrill of the hunt with some substantial savings on great groceries, then Fresh to Frozen is for you. You just never know what bargain you'll find when you walk in. LOVE Fresh to Frozen! And now that everyone's ready to run out to Fresh to Frozen, y'all be sure to leave some for us 😀 lol


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Three More Games Added to Xbox One Backwards Compatibility Program
More Xbox 360 games are now playable on Xbox One; see the newest additions here.
More Xbox 360 games are now playable on Xbox One; see the newest additions here.
Headliners join arcade FaceBreaker, NFL Head Coach, and downloadable titles Pirates vs. Ninjas Dodgeball, Shred Nebula, and My Aquarium.
We take a look at Shred Nebula for the Xbox 360.
CrunchTime Games' space-faring overhead shooter rockets onto Microsoft's online arcade next month for $10.
On this week's episode, we show off a number of games including Age of Booty, Flock, Shred Nebula, Metal Slug 7, Mega Man, Perfect World International, and more!
CrunchTime Games took the lid off its new space shooter for Xbox Live Arcade, and we got the details.
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text:
I got the black cotton flannel to interline the sleeves from Vogue, then washed and dried it twice
I purchased the black lambswool to interline the body from Bergen tailors supply in New York.
The buttons came from an old coat I made and were originally from ebay.
I had a whole block of weekend free time to devote to my new winter coat:
The finished garment measurement on the tissue for the smallest size I had (a 16) was a bit big (my bust is 42), so I graded the pattern down to a 14 (making it 2 inches smaller). I also shortened it 4 inches.
Isn't it beautiful!!
It fits my husband better than it fits me!
I was so mad at myself for falling for this pattern. I should have known better. I did want a coat with a bit of ease so I could wear it over a light jacket or sweater, but this is ridiculous! The drop shoulder plus any pattern Vogue says is loose-fitting is a really bad combination for me. Maybe a size 10 would have worked, but I am going to just have to forget about this one.
I am so glad I made a muslin and didn't ruin my good fabric.
After my epic fail, I wore my nice cozy slanket while doing my online shopping. Sure is warm. I did find a nice coat pattern on ebay. I hope I'll have better luck next time.


text: The odd-shaped block at Canal and Varick Streets is, in some ways, an architect's dream. Even the nearby Holland Tunnel entrance, nominally a downside, ensures that whatever goes up there will be visible on all sides. The owner, Trinity Real Estate, cleared the site earlier this year, and says it'll be used as a sculpture park until plans firm up. (There's already a small plaza next door, Juan Pablo Duarte Square, with a statue of the Dominican hero.) New York asked four architects to come up with ideas for the plot (which, we will admit, faces our offices). We required only that the result include a residential component and that it more or less meet zoning requirements.


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I'm over the moon excited today. My good friend and review partner has a new book coming out. I'm showing off the cover. I love it. I can tell the story is going to be deep and and a bit creepy.
Release date: February 7, 2015
Genre: YA/Haunting Mystery
Sixteen-year-old Leocadia arrives home from school to find her mom's bloody body. Unaware that the killer still lingers, she rushes to her mother's side, only to be grabbed from behind and then everything fades to black.
After a year of retrograde amnesia and battling personal demons,
Leo's dreams are getting worse—she's starting to remember. More
bodies are discovered and they seem to be oddly linked to her
mom's unsolved homicide.
When Leo allows her friend, Henry, to drag her to the haunted
Lucien Mansion, misty ghosts appear, ghosts that just might lead
her to her mother's murderer.
Will Leo let her memories threaten her into a relapse or, will she
fight to find her mother's killer – only to become his next victim?
On February 7th Cathrina is having a Release Party on FB: It's for one hour only. There will be a Giveaway and ebook Prizes. If you wish to join here's the site:
Contact the author at:
Contact the author at:
Twitter: https://twitter.com/cathconstantine


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Building Inspector Wanted…
Hi friends, anybody used a great building inspector recently? We are on the look out for super cool service providers to refer business to.
If you have had a fantastic experience with anyone in the property industry let us know so we can add them to our community of preferred service providers.
Send a link to [email protected] or post the details on here.
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Added to your favourites!
Did you know... You can enquire about multiple properties via your favourites page using one enquiry formView Favourites
Celebrating Christmas with family and friends away from home is becoming increasingly popular, so more properties are opening their doors to welcome guests during the festive season.
Book your Christmas Holiday now, before it's too late!
Christmas is a fantastic and exciting time of year for a family to get together. As a traditional season of meeting up and getting festive in large groups, what better way to really push the boat out, than to rent a beautiful holiday house.
It is natural that large holiday homes present the perfect opportunity to experience a family festive celebration. A spacious period home, classic English Manor or Scottish castle is a picture postcard ideal for many holiday makers looking to get away for Christmas.
When should I book my Christmas Holiday?
Christmas and New Year are two of the most popular weeks in the calendar year for visitors wishing to book properties, particularly the self catered ones.
We experience first hand, the disappointment by clients when they leave it too late to book a property over the festive period. As demand often exceeds supply, we highly recommend you organise yourselves well in advance if you are considering going away for Christmas.
Will a Christmas tree be supplied?
Most properties supply a Christmas tree if requested inclusive of the rental price. Some may decorate them for you, others may prefer you to bring your own lights and decorations. It is best to check with the office staff if you have any particular requirements.
Can I set off Fireworks?
As many of our properties are surrounded by farmland, it is always a matter of courtesy to advise Scotts if you wish to let off fireworks during your stay. Some farmers may need to move stock if practical. A small number of properties will not allow fireworks displays on their grounds for insurance reasons. If in doubt, please check with the office.
It is very easy to get carried away with the romanticism of Christmas, but it's worth remembering that planning for a Christmas holiday away is always a busy and exciting time, and there are plenty of things to think about. In the bustle of trying to remember what to take with you, don't forget about what you leave behind. Before embarking on your holiday away, there are a few things to remember to do to keep you and your home safe and sound.
Before you leave the house, make sure you have packed some 'must have' items that you may not want to be without! Take some medical provisions, because Christmas is a horrible time to be ill. Some cold and flu remedies, paracetomol, and a bottle of calpol for the kids is a must.
Don't forget batteries and chargers. There are so many electronic devices around these days, don't get caught short with a flat I-Pad battery, or a fancy new toy that can't be used.
Do you need your phone book? Most people have all the contacts they need in their phones these days, but just have a think before you leave, is there any one you would want to call to wish a Merry Christmas? And do you have their number?!


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Uganda scored a 78 per cent score, coming hot after South Africa and Columbia who also scored same percentage from the study.
Kenya received 86 per cent of the total possible points across all four dimensions, ranking first out of all countries surveyed, for the third time in a row and was followed by Brazil and Mexico at 79 per cent.
The 2017 report assesses the countries' financial inclusion ecosystems based on four dimensions of financial inclusion: country commitment, mobile capacity, regulatory environment, and adoption of selected traditional and digital financial services.
The report examines key developments in the global financial inclusion landscape, highlights selected financial inclusion initiatives within the 26 FDIP countries over the previous year, and provides targeted recommendations aimed at advancing financial inclusion.
"Countries have increasingly recognised that advancing access to and usage of affordable, secure formal financial services can contribute to their development objectives," said FDIP authors Robin Lewis, John Villasenor and Darrell West.
Country Commitment
Uganda scored 100 per cent on country commitment which looked at variables such as national outlook and participation in international financial inclusion-oriented organizations or networks, existence of a comprehensive national financial inclusion strategy which is in development and importantly the existence of a consumer protection framework regarding financial services. Kenya managed to gunner 86 per cent on country commitment in comparison.
Regulatory Environment
The state of the regulatory environment also earned Uganda a whooping 94 per cent being assessed on financial institutions' contract with other legal entities to serve as agents to provide financial services, eligibility of mobile network operators to apply for licenses or other formal approval from the regulator to lead mobile money deployments, regulations on e-money and Mobile Money platform interoperability which MTN and Airtel Uganda already have in place.
Mobile Capacity
This dimension of financial inclusion assessed market penetration with respect to unique subscribers as a percentage of the total subscribers in the market divided by the total population at the end of the period. Other factors included smartphone adoption rate, availability of bill payments, international remittances and merchant payments via Mobile Money. Uganda managed 78 per cent in this category.
Uganda scored very poorly getting below 5 per cent in the areas of credit and debit card use as well as percentage of adults utilizing online bill payments and purchases. It also managed an average of 35 per cent for mobile money account penetration among lower-income adults and women. And an average of 25 per cent for borrowing and saving from a financial institution. This category earned a total of 58 per cent for Uganda.


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I am really enjoying the following book:
Hand to Hand Fighting - Karate / Tae-Kwon-Do
book is designed for use by US Army Special Forces and is meant as a
training manual. It is not a fluffy martial arts book full of colour photos (all the photos in this book are greyscale) designed to boost the ego of the reader - it is a no nonsense military book on the subject of hand to hand combat.
It also includes a section on how to defend yourself from knife, gun and rifle attacks - in order to pinch their weapon and gain the upper hand.
Price - $12.99.
I got it from an army surplus store and it was totally worth it. I am going to reread it again when I am done. And practice much of the contents during my spare time, possibly adding them as a permanent feature to my fitness routine.
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Wednesday, July 10, 2013
Hand to Hand Fighting US Army Special Forces
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Like most Americans, I will be busy with family stuff today, although I will probably check in a couple times to respond to comments from readers in the rest of the world. But I wondered, "What could I do for a Thanksgiving-themed post?" Killer turkeys have probably already been done. Killer balloons, maybe not, but also not very useful. But then I thought: what about rules -- good rules -- for eating or drinking too much?
Clearly, this is a job for the reaction roll table.
|Overindulgence Reaction
|"I can't believe I ate the whole thing!" Move 3, penalty on actions,
|"I'm stuffed/sloshed to the gills!" Move is halved.
|Pleasant, ordinary meal or round of drinks.
|"I'm good for another go!" Any exhaustion or fatigue is dispelled.
|"Woo-Hoo!" Restore 1 point of damage.
Where appropriate, characters rolling a 2 may have to save vs. poison or pass out. Or, in some cases, save vs. actual poison. There may be additional effects depending on the substance ingested or the race of the character; for example, any result below 6 for alcohol should probably include the effects of a Confusion spell.
Similarly, the GM can modify the roll based on quantity of food/booze or other reasons. Hobbits (not halflings) get a bonus on the roll. Dwarves get a bonus for booze specifically. If players make a roll, then opt for another round of eating or drinking, halve the roll (or roll 1d6,) If you try to eat like Gord in Gary Gygax's first book, you wil, eventually, succumb.


text:
Climbing wall inside the American Mountaineering Center - 710 10th Street, Golden, CO 80401
Please bring your own harness, belay device and climbing shoes (approach shoes or even normal athletic shoes work just fine on this wall). No chalk.
Logan Chandler - email@example.com or you can also sign up on the Trailblazers page: https://www.facebook.com/groups/CMCTrailblazers/
FREE open climbing night for the Trailblazers: CMC's group for ages 20-40!
710 10th Street, Suite 200
Golden, Colorado 80401
Local Calls 303-279-3080
Toll Free 800-633-4417


text:
Park Releases Final Prairie Dog Management Plan
June 07, 2006
Tom Farrell, 605-745-4600
Acreage of prairie dogs at Wind Cave National Park will remain in the 1,000 to 3,000 acre range as a result of a recently completed environmental assessment. The National Park Service Midwest Regional Director Ernest Quintana recently completed the planning process by signing a Finding of No Significant Impact (FONSI) for an environmental assessment written to manage the park's black-tailed prairie dog population.
Four alternatives were analyzed in the environmental assessment, with the chosen alternative representing the mid-range of acreage alternatives. Currently, there are approximately 2,200 acres of prairie dogs in the park which contribute to the State of South Dakota's goal of 168,000 acres of prairie dogs statewide.
"In this alternative, the size of prairie dog colonies in the park will be maintained at a sustainable level, for both the long-term viability of the prairie dog population and the availability of forage and habitat for other species within the park," said Dan Roddy, biologist for the park.
Incidences of prairie dogs dispersing beyond park boundaries will be addressed on a case-by-case basis in support of a good neighbor policy. Available management tools include habitat management, non-lethal control, and lethal control tools.
For more information, and for an on-line copy of the FONSI, visit https://parkplanning.nps.gov/wica. Printed copies of the FONSI are available for review at the Custer, Hot Springs, and Rapid City libraries and at the park visitor center.
Last updated: October 26, 2016


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|By Jon Herskovitz1/5 |By Jon Herskovitz
|By Jon Herskovitz2/5 |By Jon Herskovitz
|By Jon Herskovitz3/5 |By Jon Herskovitz
|By Jon Herskovitz4/5 |By Jon Herskovitz
|By Jon Herskovitz5/5 |By Jon Herskovitz
By Jon Herskovitz
AUSTIN, Texas (Reuters) - The primal confluence of food, flames and family simmers in high definition in "Barbecue," a globe-trotting new documentary and cinematic love letter to cooking meat over an open fire.
The feature-length movie filmed in 12 countries had its global premiere at the South by Southwest film festival in Austin, Texas, not far from some of the barbecue joints that inspired Australian filmmaker Matthew Salleh and his partner Rose Tucker to explore the globe's shared barbecue culture.
"Barbecue is this perfect combination of tradition, community and culture," Salleh said in a weekend interview shortly after the film's debut.
"We just started talking to people and everyone was passionate about their country's version of barbecue," Salleh said over a plate of barbecued brisket, beef rib and sausage in Austin.
"In the six or seven months that we researched it, we were pitched by people who said 'you have got to come to my country,'" he said, adding they are shopping for a distributor for the film.
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He and Tucker went on a 200-day shoot that took them from Sweden to Uruguay, with stops including Armenia, Mongolia, Japan and a refugee camp on the Syria-Jordan border.
While the barbecue craft varied from the hot stones used to cook marmot in Mongolia to the pit used to grill goat in Mexico, the message in each trip was the same. Communities and families came together around the food.
"We have vegetarians who have seen the film and you cannot deny the cultural significance in the world of eating meat over the fire," Salleh said.
The film starts in South Africa, where racial divides of apartheid still scar the country, and shows the separate and similar barbecue tradition among blacks and whites. It includes Philippines family gatherings around roast pig and exiled Syrians trying to recapture a bit of home at a shawarma shop in a refugee camp.
After their trek, Salleh and Tucker says they have received gentle ribbing from people asking why their country's barbecue did not make the cut and a persistent question of what barbecue was best.
"At this point we begin the politically correct process of saying how we loved them all," Salleh said. Tucker added the answer can be more about the experience, then the taste.
"We had some of the best experiences in Armenia where you drink vodka with the first bite of meat," she said.
(Reporting by Jon Herskovitz; Editing by Richard Chang)


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Noella - Escape the Planet is a point and click adventure type escape game.
In this game, you need to escape from the planet called Noella by searching around to find items and solving puzzles.
Good luck!!!
Play Noella
Walkthrough - Guide - Solution - Cheats
Click on ship
Click by dead guy's armpit and get gun
Click on gun in items
Enter door on left
Shoot creature by clicking on it
For the panel, you need to have
from top, clockwise:
#1: white, black, pink
#2: black, pink, white
#3: pink, black, white
Click Enter.
Leave ship and click on the dip in the edge of the cliff
Click on dead guy.
Click on the area above his hand.
Click on cave space.
Take silver thing.
Go back to ship and enter.
Click on silver thing in Items and insert in little circle on shelf(?).
Go to little screen in front of chair and enter code on the front of the ship (c782).
Online Games point-and-click walkthrough
Noella - Walkthrough
Noella - Escape the Planet is a point and click adventure type escape game.
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I should tell you why I do my own framing. The main reason is cost. The second reason is having spent so many hours working on these projects, I really don't trust anyone else to have control over them.
Here's what I got and what it cost to do both these Santas.
From PictureFrames.com:
- Frame x 2
- Mats x 4
- Foamcore x 2
- Acrylic x 2
- Framing hardware x 2 (there's always left over clips, which I do save)
Additional costs would be the wrapping paper backing and the double-sided tape. That's less than $2. The last frame I purchased in a frame shop cost me $90US and that was just the frame! No mats, glass, foamcore or hardware and I still did all the work!
Now on to the framing!
I pin my projects for framing rather than lacing. Either way is fine, I just prefer the security of the pins.
You would need these items:
- Frame
- Mat(s), optional
- Spacers (only if no mats)
- Foamcore
- Acrylic or glass, UV protective is fine; non-glare will just make the stitching look blurry and isn't protective
- Stainless Steel Pins (even if lacing)
- Counting pins or large headed pins
- Thread - I use hand-quilting thread (unwaxed dental floss is also an option)
- Needle - this should be a sharp rather than blunt
- Screwdriver
- Wire cutters
- Awl, optional but very useful - I have misplaced mine so I had to do without
- Ruler
- Some sort of blade - I use a quilting rotary cutter but a utility knife would work too
- Framing clips - what holds your piece in the frame, style may vary
- Wire
- Eye hooks (2)
- Brown paper or wrapping paper
- Camera if you want a photo of your framed project (you will take a photo during the framing process)
- Your completed cross stitch, of course
This is the most important part of the process. If you mess this up, it won't look good in the frame no matter how gorgeous (read, expensive) the frame is!
Oh, I should mention that in the photos Santa might appear to be having an identity crisis. He couldn't make up his mind if he wanted to be wearing red or black. Just depends on how photogenic he was feeling at the time.
Lay your piece face up on the foamcore:
Get it approximately centered on the foamcore. Since I decided that I didn't want my "signature" to show, I'm ignoring it for this entire process.
Place a large-headed pin in the center of each side. This is just to keep it from shifting while you measure.
The order doesn't really matter as long as you do opposites together (top/bottom or left/right). I measured from the bottom of the stitching to the edge of the foamcore. I then measured from the top of the stitching to the edge of the foamcore.
In the photos, the bottom was about 2.375" and the top was about 2.5". I removed the top and bottom pins to allow me to move the fabric slightly toward the top. That increased the amount at the bottom and decreased the amount at the top to even it out. It was a small amount so I didn't have to remove the pins on the sides for the little adjustment. I replaced the top and bottom pins.
I measured the remaining sides of the project and make any necessary adjustments.
Now this is where I'd say I'm OCD...I'm not, but I seem to have the occasional OCD moment.
Using the counting pins (or other large-headed pins), count from the outside edge of your stitching to the edge of your foamcore. Ok, I didn't use large-headed pins to count but I was too lazy to get off my butt and grab some. In this photo, the uppermost twig is the stitching at the edge of the design. My count is based from the hole that lines up with the twig. The first pin is 25 holes and the second is at 50 holes from the stitching. Using multiple pins helps keep you (me really) from losing count.
I kept counting until my pin lined up with the center of the foamcore edge. It ended up being 62 holes. I then counted 62 holes from the opposite side of my stitching towards the center of the foamcore edge. Hmm, 62 holes was a tad bit short so I went to 64 which was still a tiny bit off from the center of the foamcore (where the real, large-headed pin is). Switching one side to 64 meant that I had to change the other to 64 as well. Making that adjustment let me put pins in the foamcore on both sides at the same distance from the stitching.
I moved my large-headed pins into the "64 hole" and then counted in the other direction (in this case, top and bottom). The measurements top and bottom also came out to 64 holes. This is not always the case! I was really surprised with this one because the fabric is true linen which isn't always "square" due to the fabric threads being uneven thicknesses. Again, I switched my bigger pins to the appropriate hole.
Now comes the
The pins I use are stainless steel because they won't rust! They are 1" long with small but noticeable heads.
I usually start on one of the long sides, in the middle. I work from the middle to one corner, then from the middle to the other corner. I don't measure how far apart they really are but I'd guess ½" - ¾". Until I have them all in along one side, I leave them sticking out. I don't think there is a particular reason why I do, I just do.
Once they're all spaced, I push them in. Sorry it's hard to see in the photo. I think the little beggars are camera-shy.
I then do the opposite side the same way. I think I do the long edges first because when I get to the third side, I'm definitely more than half-way done. I do the same along the other two sides and voila! the pinning is done.
Hmm, there's all that extra fabric What to do, what to do? Truss it up I suppose!
I started at the bottom left corner. Folded the point of the fabric in.
Folded the excess from one side than the other. This is when I needed the needle and thread. I use a single thread of hand-quilting thread. I ran it through the folded fabric and tied a knot. I should mention that I'm a bit lazy. Rather than have to cut more thread part-way through, I use a really looonnngg length of thread - probably close to two-yards long.
I then went to the bottom right corner and folded the fabric the same way. I ran the needle and thread through the corner fabric like in the photo above. I then zig-zagged my way up the sides (the narrow way) until I reached the top corners. I do really big zigs and zags. If you are ultimately lacing your project (removing the pins), you would want to space your zig-zags close together like the pins were and keep the thread as tight as you can.
When I got to the first top corner, I folded it like the previous corners and anchored it with the thread. I then started zig-zagging from top to bottom (the long length), until I reached the remaining corner. The corner got folded, threaded and then the thread got knotted off. I only lace to keep the excess fabric from flopping around like a fish - my pins stay put. If you are lacing your project, now is when you would remove the pins.
Here it is from the front, looking all tidy.
Whew! The hard part is done!
Framing the Piece
When I got the box containing my 2 frames, they were well-packaged! I had a little "prep work" to get them ready to use. The acrylic had a protective sheet on each side which needed to be removed. The mats were protected by the 2 pieces of foamcore so I had to separate them (they were lightly taped).
I took the frame and put it face down on my work table. I then popped in the appropriate mats (white/maroon) and the red Santa. At this point, that's all I did - no acrylic, no clips, nothing else. I turned the whole thing over and took a photo.
Sometimes, I hang it on the wall to photograph it but this was easier at the time. The reason that I photograph it now is no glare or reflections! It is completely framed/matted and perfectly photogenic.
I disassembled it, put in the acrylic, added the mats, and then popped in Santa. Now is the time I really looked at it and checked everything. I made sure there are no fingerprints or dust on the acrylic (the second one took forever to clean - there was a lot of fuzz and fingerprints). I checked to make sure the mats were aligned properly (they are two separate pieces so they do shift). I made sure Santa had the same amount of space all around him.
Then I carefully turned him back over and clipped everything into place. The clips I used came with the frame.
These clips rock! They are made for one end to fit in the channel on the frame and the other puts just enough pressure on the piece to keep it in place. They also don't need any special tools, just thumbs!
I started on the long edge and put one near the corner, one near the center and the last one at the corner. Across the narrow edge, I only used one in the center.
I like to make my framing look as tidy as possible. I also don't want dust or anything else to get to my fabric so I like to really finish off the back. I started by putting two-way (2-sided tape) all the way around the back of the frame. I like to get it as close as possible to the edge. Since this tape doesn't come in contact with my fabric, I actually use the tape from my trophy shop. It's not acid-free but like I said, it doesn't touch my fabric. It's the yellow around the edge.
I could use regular brown paper like grocery bags or parcel wrapping for mailing, but this is a Santa - Christmas wrapping paper!
I cut a piece slightly larger than the entire frame. The paper is actually sitting on the frame here:
I take it off and peel the yellow paper off one of the tape strips. I started with one on the narrow edge. If you look closely at the photo of the tape, you will see that the long strips go to the edge of the frame and the narrow strips fit between the long ones. By pulling a narrow one first, I still had easy access to the paper on the long strips. You'll thank me later for that tip!
I put the Christmas paper back over the frame and carefully pressed it onto the one exposed tape working from the center out. Then I uncovered the tape on the other narrow edge and keeping the wrapping paper taut, I pressed that end down. I then did the same to the two remaining sides.
Now I needed to trim the excess paper. This is when it's handy to have other hobbies - you can "steal" the tools from them. I used a quilting ruler and rotary cutter. If you are a quilter, don't freak out - I have a rotary cutter that I use just for stuff like this and a separate one for actual quilting projects.
First I just finger-pressed the paper over the edge of the frame so that I could see where the edge was.
I then put my ruler as close to the edge as possible and whacked off the excess with the rotary cutter.
Now I need to measure for my hanging wire. I took these photos before I put the paper on because there was better contrast. I measured the overall height of the frame. You always measure from top to bottom for this step even if your piece is wider than it is tall. My frame is just under 16½" tall.
Then I measured from the top edge, down just a bit over 4". The rule of thumb for hanging with wire is to be down ¼ of the overall height. 16.5 ÷ 4 = 4.125 (or 4 1/8"). For the photos, I made a mark down 4 1/8" from the top on both sides. Normally, I just measure down and use my awl to make a pilot hole.
Now here's a tip from the sad voice of experience. As you can see in the above photo, my mark is near the edge of the frame. That's not a problem as long as you check your frame first! If you have a frame that has a profile like this:
and you tried to place the screw/eye hook for your hanger in the thinner area on the left without checking the length of the screw vs. the thickness of the frame, your screw might end up poking out the front of the frame. Yes, I have had that happen. Thankfully once was all it took for me to learn that lesson!
Now I attached the hanging hardware. In this instance, they are eye hooks. The screwdriver is much easier to hang on to than that tiny little eye hook. So 'round and 'round goes the screwdriver. I made sure that the base of the eye just barely came into contact with the back of the frame.
The eye should also end up parallel to the side of the frame.
Next I had to attached the wire to the eye hook. I made a loop near the end of my wire and inserted it in the eye hook.
Then I took the end of the wire and fed it through the loop and pulled it snug. The excess end wire got wrapped around the main wire. You may end up using the plier part of your wire cutters to twist the very tip as close to the main wire as possible to prevent it catching on the paper, your finger, etc.
Most of the time, I have way more wire than I need - hence the wire cutters. I tightly pulled the wire across to the other eye hook then gave it a little slack and made a loop like before. Once I'd done this, I could see how much excess wire I really had and I cut off the extra.
Just in case you don't know what wire cutters look like, here you go:
These are needle-nose pliers with a wire cutter. The very base of the "v" of the pliers is the wire cutter.
Here is how much slack I left in the wire. It doesn't need much, but it does have to have some or it would be impossible to adjust the way the frame hangs.
For reference, the top of the frame is on the left.
Now for the Santas to finally be seen together:
Happy Stitching!


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Journal Focuses on the legacy and impact of Anthony Cerami's groundbreaking work on diabetes
Manhasset, NY – Molecular Medicine, the peer-reviewed open access journal published by Feinstein Institute Press, today released its 20th anniversary edition. This special commemorative issue highlights the career and scientific impact of Anthony Cerami, PhD. It includes reviews about the influence of Dr. Cerami's research on the scientific careers of renowned leaders in the fields of parasitology, hematology, immunology, metabolism, therapeutics and molecular medicine. Dr. Cerami, CEO of Araim Pharmaceuticals, is best known for developing the HbA1c (A1C) diagnostic test, the current gold standard for diagnosing diabetes.
Dr. Cerami's interest in the underlying conditions of diseases developed at an early age, when his mother developed diabetes. He witnessed first-hand the complications and suffering caused by the disease. This inspired Dr. Cerami to study the cause and consequences of diabetes to best explore viable treatments.
"As I look back over the course of my work, it is easy to see how ideas, observations and discoveries are connected," said Dr. Cerami, editor emeritus of Molecular Medicine. "The basic biological questions I asked as a youth have been the framework for most of my scientific work. My perseverance was encouraged enormously by mentors, students, collaborative colleagues, and the desire to understand and develop therapies that might help patients has obsessed me my entire life. There is no map for the unknown, but that is the fun of it."
The issue includes an introduction by Kevin Tracey, MD, president and CEO of the Feinstein Institute for Medical Research, and editor emeritus of Molecular Medicine.
"Students in molecular medicine and active investigators pursuing basic science in the hopes of improving human health will find inspiration in these accounts," said Dr. Tracey. "Dr. Cerami's persistence and passion fed his ability to move beyond observations of the natural world to perform experiments that continue to produce new diagnostics and therapeutics. Future generations of molecular medicine researchers can learn from his approach."
Another major focus of Dr. Cerami's research has been the identification of tumor necrosis factor (TNF) as a key inflammatory mediator of tissue damage caused by a wide variety of diseases. The current success of anti-TNF therapy as a disease modifying treatment for rheumatoid arthritis, Crohn's disease, and psoriasis, is based on this discovery. This work has also culminated most recently in the engineering of a peptide, ARA 290, as a general antagonist of inflammation and an activator of tissue repair. The issue can be read in its entirety on molmed.org.
About Araim Pharmaceuticals, Inc.
Araim Pharmaceuticals, Inc. is a privately held biotech company founded in 2006 engaged in developing novel treatments for devastating injuries and chronic diseases underserved by current therapies. Their lead compound, ARA 290, is a novel 11 amino acid peptide engineered to specifically activate the body's natural repair system via the innate repair receptor that is present only following injury. ARA 290 activates anti-inflammatory, tissue protective, and reparative signaling pathways. The short half-life of ARA 290 coupled with the restricted expression of the innate repair receptor functions as a dual safety system to avoid potential side effects. Clinical trials evaluating ARA 290 for treatment of neuropathic symptoms associated with diabetes and the orphan disease sarcoidosis are currently ongoing in the US and Europe. ARA 290 has received orphan drug designation in the US and in Europe, as well as Fast Track designation in the US for treatment of small fiber neuropathy in sarcoidosis. For more information, visit www.araimpharma.com.
About Molecular Medicine
Molecular Medicine is an open access, international, peer-reviewed biomedical journal published by the Feinstein Institute Press. Molecular Medicine strives to understand normal body functioning and disease pathogenesis at the molecular level, which may allow researchers and physician-scientists to use that knowledge in the design of specific molecular tools for disease diagnosis, treatment, prognosis, and prevention. To learn more, visit www.molmed.org.
About The Feinstein Institute for Medical Research
Headquartered in Manhasset, NY, The Feinstein Institute for Medical Research is home to international scientific leaders in many areas including Parkinson's disease, Alzheimer's disease, psychiatric disorders, rheumatoid arthritis, lupus, sepsis, human genetics, pulmonary hypertension, leukemia, neuroimmunology, and medicinal chemistry. The Feinstein Institute, part of the North Shore-LIJ Health System, ranks in the top 6th percentile of all National Institutes of Health grants awarded to research centers. For more information, visit www.FeinsteinInstitute.org.
Media Contact:
Terry Lynam, Vice President of Public Relations
(516) 465-2640


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The "March for giants" campaign, which is the first to use digital out-of-home in this way, was an International Winner in the 2016 creative digital competition run by Campaign and Ocean Outdoor.
Corporate donors will be invited to create an elephant decorated with their logo and brand colours for £5,000, the cost of protecting an elephant for life
Individuals and companies will be able to generate their own branded adult and baby elephants to swell the herd which will march in real time across Ocean's high-impact digital outdoor sites in the UK and across some of their Alliance partners screens in Hong Kong and New York. The campaign supports the work of the conservation organisation Space for Giants.
Corporate donors will be invited to create an elephant decorated with their logo and brand colours for £5,000, the cost of protecting an elephant for life in the wild in Africa. Individuals will have the chance to name a baby elephant for £5 or sign up to an annual subscription of £5-a-month, which will protect an elephant for one year.
The virtual herd will be created and grow online during March before being released across five giant digital out-of-home screens operated by Ocean. These include Tsim Tsa Tsui in Hong Kong, Times Square in New York, Westfield Stratford City in London, The Media Eyes at Grand Central in Birmingham and the Manchester Printworks.
The march will begin in Hong Kong on March 23 before moving to New York and on to London on March 24. It will continue to Birmingham on the following day and end in Manchester on March 26.
Corporate donors already signed up to the "March for giants" include National Express, Givergy, Made by Riley, The Delta Group, Westfield, Chantecaille, BCD Travel and First Avenue.The march will be supported in social and broadcast media by a network of celebrities, social influencers and international news networks across the world.
William Thacker, creative director at 18 Feet & Rising, approached Space for Giants after the 2016 Great Elephant Census reported that a third of all African elephants have been wiped out in the last seven years.
The creative digital competition run by Campaign and Ocean rewards creative concepts that are later brought to life on Ocean's digital screens. "With the competition open, we saw an opportunity and real possibility of securing some global media on a scale significant enough to make a difference to an extremely serious and unreported issue," said Thacker. Digital agency Popped also worked on the campaign and DOOH.com worked on digital integration.
"To see this march of virtual elephants unfold across the world is a massive ideas made possible by an incredible team," said Tim Bleakley, chief executive of Ocean Outdoor. "The creative concept and cause are worthy winners of our creative competition."
Source: Campaign
"This is a worldwide media first for our industry, supporting an amazing charity and delivered across multiple channels simultaneously. This is why I approached '18 Feet & Rising' at the Ocean award ceremony offering our complete and free support" Andrew Phipps-Newman – CEO DOOH.com
Other Articles:
Evening Standard
Creative Pool
Daily DOOH
Screen Media Daily
Mundo Marketing
Communidad EME
Net Imperative
Daily DOOH


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Foligno, Italy – Bundleecious' first 2013 Bundle includes 5 amazing Apps for your Mac and a 3 Month License of online backup system Backblaze for all the first 1,000 customers (Total Value: $140.00 USD – Bundleecious Price: $9.99 USD).
What's included:
* Backblaze 3 Month License ($15) – only for the first 1,000 customers: Backblaze backs up everything on your computer, continuously and securely. You can restore your data at anytime by downloading a zip file or having a USB hard drive sent.
* MacPilot from Koingo Software ($20): Easily enable and disable hidden features in Mac OS X, optimize and repair your system, and perform numerous routine maintenance operations with the click of a button!
* Speedy from Apimac ($15): Speedy is a powerful utility that brings you the power to quickly launch everything you need for your everyday work as documents, folders, apps, sites and AppleScript workflows.
* Clipbuddy from Ondesoft ($30): ClipBuddy is the ultimate Clipboard Extender on Mac OS that records everything you cut or copy to the Mac Clipboard, and provides multiple ways to view, edit and paste them to any app.
* CollageFactory Pro from Softease ($30): CollageFactory Pro is a powerful combination of photo collage maker and greeting card creator. You can make photo collage and holiday e-greeting card effortlessly.
* Audio Recorder from Ondesoft ($30) – Free For Anyone: Ondesoft Audio Recorder is a powerful and easy-to-use Mac audio recording software, with it, you can capture and record audio from any application on Mac and save to any audio file formats.
Pricing and Availability:
Any Mac user can get all these apps for only $9.99 from the Bundleecious website. Bundleecious Bundle is a very limited time offer that will expire on 17th of March.
Ondesoft Audio Recorder Giveaway
Launched on May 2010, Bundleecious is quickly become one of the most reliable bundle of apps for Mac. Proudly created by two italian guys, Gabriele Contilli and Riccardo Caprai, Bundleecious is an indie bundle which aims to present innovative offers for all Mac-users. With thousands of customers all around the world, Bundleecious is one of the best way to buy applications for Mac. Copyright (C) 2010-2013 Bundleecious. All Rights Reserved. Apple, the Apple logo and OS X are registered trademarks of Apple Inc. in the U.S. and/or other countries. All other trademarks are property of their holders.


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Find a wedding photography course that will suit you.
One of the best ways for you to be able to get ahead of the competition one way or the other would be if you could get some kind of formal training that will really give you that competitive edge for as far as photography skills are concerned. This is something that you will need to tread carefully with though because it is something that can cause a serious dent in your finances. It will also take up an awful lot of your time and efforts so you have to proceed with caution and do as much research as you possibly can. Check out the photography styles that will suit you the most and those that you are interested to develop at some point or so.
Check out the background of the teacher who will be conducting the course or the classes. You will need to go for someone who has the reputation and the experience to back him up and to make sure that he has all of the right credentials for it. Another thing that you will have to figure out would have to be the aspect of the schedule. Find a schedule that works for you and that you will not come across a lot of troubles to work out. If it is not possible for you to attend actual physical classes, then you should check out the possibility of online photography classes instead.
Get someone to be your mentor for your wedding photography interests.
You will still need a guiding hand, someone who has been there and done that in the field of wedding photography. This person can be your constant source of advice and inspiration as well so that you can really steer your career towards the right direction at the end of the day. Try to see if you can have someone meet you at least around once a month. If you cannot find anyone who can personally meet with you, then you can possibly resort to following the blog of a wedding photographer Warwickshire who inspires you the most.
Get as much wedding photography experience as you possibly can.
You cannot bring in any clients without having some kind of experience first. This can be tough to go with in the beginning but for as long as you are not picky with the little projects that you get at first, then you should be able to get things running for you and built your career up slowly and bit by bit. If you have to, then try to see if you can assist in the wedding events of other more established and seasoned wedding photographers out there. It may not be as glamorous as you would like it to be at first, it is still a pretty good start and it will steer you towards the right direction.
Constantly surround yourself with people who support you and your wedding photography dreams.
Wedding photography is a tough dream to chase after. You will need all of the help that you can get your hands on.


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Tag: boxing
Box It Out: Q&A with Noah Neiman, Co-Founder of NYC's Rumble Boxing Studio
In a sea of boutique fitness studios, Rumble is the latest way to get in a sweat.
Here's What It's Like to Take a Class at Gotham G-BOX
Boxing can be for everyone with the new Gotham G-BOX class.
Acclaimed Fashion Photographer Ben Watts on Wellness
In the fast-paced society that we live in, Ben reminds us to take time to relax at the end of the day.
The 6 Essential Pieces of Boxing Gear
It's not enough to be good at something anymore - you want to look good doing it. Here's our roundup of an enviable boxing kit.
The 4 Fundamental Boxing Exercises
Boxing requires a unique combination of footwork, hand eye coordination and power. Each dimension requires a different training protocol - we break it down for you to get you fight ready.
WellPath Inspiration: Bruce Lee
The man most responsible for popularizing martial arts in the western world and who remains a source of inspiration to anyone who aspires to achieve the impossible.
The Reemergence of Boxing
Boxing is having a renaissance in part fueled by a renewed desire for challenging workouts and the virality afforded by social media. We take a deeper look into the drivers behind the trend.


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I am currently documenting two cemeteries which have mausoleums over ten feet tall. I can't get a clear picture of the two or three rows of markers at the top. Does anyone have any advice for me?
Would a selfie stick give you enough extra height?
No. They are 2 to 2 1/2 stories high. Maybe they have extensions for selfie sticks. I will have to check.
Yes. I have tried that. The problem is that it is hard to get a clear, steady picture with the drone. I think what I am going to do is to take a tall ladder with a selfie stick. I have never used a selfie stick before, but everyone is advising to use it, so I am going to try that. Thanks for your help!


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Filed under: Coliseum | Tags: Adrian Adonis, Big John Studd, Billy Jack Haynes, Bobby Heenan, Bret Hart, Brian Blair, Cousin Junior, Dan Spivey, Harley Race, Hercules Hernandez, Jake Roberts, Jim Brunzell, Jim Neidhart, Jim Powers, Jimmy Hart, Jimmy Jack Funk, JunkYard Dog, King Kong Bundy, King Tonga, Lanny Poffo, Lou Albano, Moondog Rex, Pat Patterson, Pedro Morales, Ricky Hunter, Ricky Steamboat, Ted Arcidi, Terry Gibbs, Tony Garea
It's time for the eighth tape in the Best of the WWF series, as we head through the summer of 1986. The cover for this volume is bright yellow and features a young star named Bret Hart. Advertised on the box are Jake the Snake versus Ricky Steamboat, Pedro Morales versus Adrian Adonis, Pat Patterson versus Lou Albano, debuts of Hercules, Dan Spivey and Harley Race. It looks like quite the impressive lineup, so let's roll the tape.
The usual opening sequence sends us to Mene Gene who talks about the WWF TV coverage, seven hours a week across the planet. That really surprised me, I thought we had a lot of TV today, but they had about the same twenty five years ago! Gene sends it to our opening match, from Madison Square Gardens! Continue reading


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Below is a map showing the results of yesterday's elections.
And here is the map of prediction market forecasts I posted on October 1st.
Florida has yet to be called, but if it goes to Obama, then the prediction markets will have accurately forecasted 50 out of 50 states five weeks before the election.
Here is how the prediction markets fared in 2004 and 2008. I'd say they nailed it yet again!
Read more of my posts on prediction markets here.


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About Us
The economics department has a strong research record, with a particular emphasis on applied economics. Its main research strengths include international macroeconomics and various areas in applied microeconomics (including international development, energy economics, urban economics, cultural economics, industrial economics). Economics was first taught in Trinity College in 1832 when Mountifort Longfield was appointed to the newly established Chair of Political Economy, a chair endowed by the then Church of Ireland Archbishop of Dublin, Dr. Richard Whately.
The Department takes great pride in its undergraduate teaching, provided through the BESS (Business, Economics and Social Studies) programme, the PPES (Politics, Philosophy, Economics and Sociology) programme and the TSM (Two-Subject Moderatorship) programme. The Department has exchange programmes with a range of international universities.
Equally important to the Department are its successful postgraduate programmes: the MSc in Economics, designed to provide the skills required to undertake advanced research in Economics and the PhD in Economics. The doctoral programme includes 30 credits of PhD-level coursework, plus the writing of a dissertation. It is the largest doctoral programme in Ireland and its doctoral graduates have positions in high-profile academic institutions (Kiel, Lausanne, Ecole Polytechnique, University College Dublin, City University London, Hamburg), policy organisations (European Central Bank, European Commission, International Monetary Fund, OECD, Central Bank of Ireland, Irish Department of Finance) and the private sector. The Department provides generous financial support for research students through a programme of teaching assistantships, as well as providing a number of research awards.
The Department has been successful in attracting financial support from the Irish Research Council, Irish Aid, Science Foundation Ireland, PRTLI, the EU Framework Programmes, NORFACE, the World Bank, Fondation Banque de France and the Institute for New Economic Thinking (INET).
Whately Professor of Political Economy Philip Lane has a high international profile in research on international macroeconomics – the macroeconomics group also includes Agustin Benetrix, Vahagn Galstyan, Paul Scanlon and Michael Wycherley. The international development group mass includes staff members Fadi Hassan, Michael King, Tara Mitchell, Gaia Narciso and Carol Newman. The department is also strong in areas such as cultural economics (John O'Hagan), energy economics (Eleanor Denny) and urban economics (Ronan Lyons). For more detailed information on research activity, see the home pages of individual staff members.


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Apple Inspired Appetizers For Fall
You all know I love good food and good drinks. But what I can not get enough of is appetizers! Seriously, I can (and have!) make a meal out of appetizers. There is just SOMETHING about yummy food in a small bite or a dip that makes me want them ALL THE TIME. If you are a restaurant and you have tapas or a small plate menu…. I am DONE. Take all my money.
With fall knocking on our door it is time to start thinking of all things Apple. Fresh apples, apple sauce, apple pie, dried apples, apple cider and culinary products featuring apples.
Honey crisp
GIANT Food Stores loves Apples as much as the rest of us. They have created a Limited Time Original product line and they have decided to feature one of my FAVORITE apples, the Honey crisp. There are over 2,500 varieties of apples grown in the US and 100 varieties are grown commercially. So for the Honey crisp to be featured, that is a big deal! Not all apples taste, cook or juice the same! So I am super excited that Giant decided to feature the Honey crisp apple. It is so good for so many reasons!
30 Apple Limited Time Originals
You will find these Apple-inspired products in the store to include in dairy, bakery, deli, floral, prepared food, household and general merchandise.
Offerings include:
- granola
- cinnamon sweet filled rolls
- apple dicer donuts (yes please!)
- honey crisp apple inspired goat cheese
- spiced apple & butternut squash bisque and apple cheddar ravioli
- Nature's Promise Apple all-purpose cleaner
- and so many more!
So let's take some of these items and make some fall appetizers with them! Enjoy them while you can because they are here for a limited time.
Chicken and Waffles – Apple style!
Start with the Apple Chicken meatballs from the honey crisp line.
They are precooked but they are still frozen. I followed the package instructions to reheat them.
Easy peasy.
Grab a bottle of the Mulled Apple Bourbon BBQ Sauce (I could eat this with a spoon!)
I think this BBQ sauce would make and EXCELLENT addition to a pulled pork sandwich. Check out how I make it in the crockpot in this recipe from when Giant had the Bacon! Moonshine BBQ Sauce in limited release back in January. Same directions…. just switch out the sauces.
Toast the Belgian Waffles and cut each one into quarters.
Top each waffle quarter with a chicken meatball.
Secure with a toothpick.
Drizzle with the BBQ sauce.
I had a small bowl of extra sauce on the side for extra dipping if anyone felt it was needed.
These are DELICIOUS!
Great for an appetizer or 'snack on dinner' as we call it.
Baked Brie
*sigh* I just want you to know I have had a love affair with baked brie from the age of 15 when I got my first job as a prep cook in a fancy country club. Brie and I go way back.
Giant has go you COVERED with this Honey Crisp Inspired Baked brie. The apples and cinnamon are already IN the pastry. The pastry is already wrapped around the brie so you don't have to break out your rolling pin to roll out pastry.
It is fool proof.
Take it out of the plastic box and the plastic bag. Pop it on a cookie sheet and bake it according to the package instructions.
I sliced one of Giant's Nature's Promise baguettes to place along side the baked brie.
To add to the apple theme I sliced up an apple as well.
Nothing goes better with baked brie than baguette and apples!
Caramel Appletini
What is appetizers with out a little cocktail?
The Just Pressed, unfiltered juice was just SCREAMING to be made into a Caramel Appletini. I love that this juice is unfiltered! All the GOOD of the apple is still in there. Just be sure to give it a good shake so you evenly distribute the fiber through the juice.
So simple, even I can do it!
In an ice filled shaker add:
- 2 oz caramel vodka
- 2 oz green apple vodka
- 4 oz Just Pressed apple juice
Shake it up and pour in a martini glass.
Garnish with a few slices of apple that have been dipped in cinnamon and ENJOY!
Grab the Honey Crips Apple Limited Time Originals while they are available. Check out more information about them on Giant's website.
Be sure to read more about my delicious post featuring Giant.