text:
Daytona Beach Attorneys / Daytona Beach Lawyers - Nursing Home Abuse /
Daytona Beach Lawyers - What to Look For
What should I look for when visiting a nursing facility?
Are there any unpleasant smells? Do residents took well groomed and clean? Does the food taste good? When you test a call bell, is there a quick response? Does the staff respect patient privacy? Does the facility allow private furniture? Are there any visiting restrictions? Is the facility clean? Do staff interact pleasantly and cooperatively with each other?
These are a few of the things the Daytona Beach lawyers of Seitz & Tresher advise you to look for.


text:
I thought id give you all a look at what happened at this lovely event in saltburn! Lillian's House was proud to be part of a fabulous vintage fair 'For the Love of'. Featuring delicious 'true' vintage stalls and lovely vintage inspired stalls it was a treasure trove of bits and pieces perfect for Christmas presents.
Here's a few snapshots of the Lillian's House stall for you to have a nosey at and hopefully enjoy...
Look out for the hand printed Christmas Gift Boxes! With a hand printed tag, string and tissue included, what a find for hassle free pressie wrapping. I'll certainly be using a few of these for family and friends this year, they will look beautuful under the christmas tree.
Miniature bird houses, pom poms and tiny arabian slippers adorned our Christmas tree this year.
Thankyou for all that came down to see 'Lillian's House'...and to all who adopted some goodies from us, i hope you enjoy them and they bring lots of festive cheer!!
A few of my favourites of the day were...
- Vicky Trainor and her wonderful vintage stationary & gifts...look @ http://www.thevintagedrawer.com/
- Northern Lights Interiors...a gorgeous eclectic mix of antique and modern antique bits and pieces. Based in Saltburn, this shop is well worth a peek in! http://www.northernlightsinteriors.co.uk/
- NJL Imaginarium, dark, gothic, victoriana, gorgeous. What a wonderful mix...look @ http://www.njl-imaginarium.co.uk/
- Bo&Bo, a quirky and sweet place to find goodies for your little ones. http://www.boandbo.co.uk/

subset: commoncrawl


text:
Show Summary Details
Quick Reference
(Dresden, 1747–1831, Copenhagen),
was from 1784 to 1813 Danish finance minister. In 1824 he became foreign minister. A humane man greatly interested in poetry and the arts, Schimmelmann, together with ...
Subjects: Literature.
Related content in Oxford Index
Reference entries
Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.


text:
Loch Ness Monster
I was asked to go to Scotland to sort out a rundown fishing hotel on the side of Loch Ness, some fifteen years ago.
Being an avid salmon fisherman, it seemed a great opportunity to chase some Loch Ness salmon and get paid for the enjoyment.
Nothing was further from the truth, I found to my horror, the hotel was a complete shambles, the woman that had obtained it, had absolutely no idea what to do and should never have been in the hospitality business, which is another story.
The bar in the hotel had a number of local fishermen frequenting it regularly. They were a trifle frosty when I first met them and after exchanging various fishing stories, they realized that I had caught a considerable amount of salmon and other fish and was not a Southerner telling them how to fish on their home territory, a mistake made by many visitors.
I sat in the bar and listened to their stories, which always have a similarity wherever you go, only the water changes. The conversation moved from fishing to the Loch Ness Monster, which made me smile very discreetly.
Having been a commercial diver and fished in some strange places for some equally curious fish, my skepticism of the Loch Ness Monster would have alienated me from some interesting characters, who were going to be my customers for the next two months.
The conversation moved from Nessie to eels and a big argument ensued, the serious fishermen were convinced that Nessie was a large eel. It is common knowledge that Nessie could not be herbivore, because there is insufficient plant growth in Loch Ness to sustain an animal of the alleged size of Nessie. Likewise, if Nessie was fish eater, the fish in Loch Ness are migratory salmon, passing through the loch, pike, slob trout and eels, none in sufficient quantities to sustain any large mammal.
The loch at the time when I was there, unbeknown to me at that time was way below it's normal level, due to a serious drought, and salmon were having trouble getting up the river to the loch and the feeder streams to spawn, making even less fish available for a possible Nessie.
My fishing locals, said that salmon fishing was out because of the low water, but I could fish for pile, slob trout (large cannibal trout normally at great depth) or eels.
Eels I have always avoided, having caught and shot, with a harpoon, a number of large congers, where the conger was more in control than I was, large ones are to, be avoided.
I duly arranged to go pike fishing late at night, the advice given to me was light a fire, wear tough boots, take a nail bar with me and keep away from the waters' edge, I thought it was a wind up.
The advice was good, on a subsequent trip. Whatever I hooked smashed up a steel trace, frightened me stiff and gave me a healthy respect for Loch Ness, I also pulled up some animal bones, which may have been thrown in the loch by a local after butchering a deer, or the deer went to close to the loch and couldn't get out.
I also saw small eels working their way up a feeder strip, searching for any available food in late evening.
This caused me to write a book called the Loch Ness Eels, which is now, published on smashwords.com.
The story is fiction based on my research into eels after my frightening experience, using the crazy antics with the hotel as a background and fishing for eels.
The facts about eels that I discovered are as follows.
Firstly, eels have never been, seriously researched since they have no real food value.
They were believed to migrate to the Sargasso sea to spawn, an area that is almost impossible to research, it is now believed that they spawn at great depth, in the deep trenches of the oceans, Loch Ness is on a fault line and very deep.
Small silver eels are migratory and do not grow to much more than a meter.
Conger eels normally live in seawater but can live in freshwater.
Male congers, supposedly grow to about twenty pounds in weight.
Female congers grow to enormous size, rod caught ones at up to 12o lbs in weight, but the Dalkey eel was reputed to be around 300 lbs and put back in the sea, caught off the coast of Scotland.
There are stories of enormous eels being caught in fishing trawls and thrown back, also stories of lakes with enormous eels in other parts of the Northern Hemisphere, all third hand without confirmation, but sufficient to warrant a comment in various books or newspapers.
The following stories were related to me by a number of Loch Ness fishermen, in addition to further research, the perpetuating of Nessie is a major tourist attraction and now a major factor in the local economy, to have Nessie converted into a very large eel is a cause for local concern.
A lady was in a rowing boat with one son and the other son decided to swim to the boat and a large eel grabbed him in shallow water and the mother beat the eel off and rescued her son, all were in a state of shock, the only eel of that size would be a conger eel.
Supposedly, there are various stories of people vanishing in reasonably safe conditions never to be seen again.
A submersible went down to a considerable depth near Drumnadrochit, the pilot came up quickly and refused to go down again, he was terrified.
Another unconfirmed story was that a very large eel got caught in the sluice on the lock gate, leading to the sea from Loch Ness, they could see the outline but it was gone the next morning.
Eels thrive in detritus, live in holes, feed in the main at night are cannibals, and have a perpetual food source, in addition thrive on rotting meat.
There is a theory that there is an outlet to the sea from Loch Ness supposedly near Drumnadrochit, the sea is more than fifty miles, but Loch Ness is on a fault line, maybe there is an underground stream going to the sea, it is all speculation.
Large migrating eels moving up or down the river Ness would be seen, or the eels would avoid the river because of the street lights from the adjoining roads illuminating the river.
If there are very large eels living in the detritus which could be meters deep or holes in the loch sides, they would certainly not be visible to any scanning equipment during the day.
Eels do not have swim bladders, like fish, but their bodies will swell up if they have been at great depth. A bloated dead or sick conger will rise to the surface as the gases expand, where it barks exuding the gases until it is back to normal, but it's body shape could appear to be enormous, then reduces and sinks to the bottom again, if it is dead it provides a food source for the remainder.
I did not publish my book, until this year, following a newspaper article about a sonar image of a massive eel like creature below a boat on Loch Ness.
The eels, if the information is correct, answers a lot of questions, but if correct raise many more.
One thing is very certain, I will not fish at night from the banks or in a small boat when Loch Ness is very low during drought conditions.


text:
Yesterday's Wondershop Series workshop was AMAZING! I am so thrilled at how well everything went and loved meeting all the amazing photographers who attended. I can't wait to give a full re-cap, but for now I'll just share this image of the gorgeous Kamra.
By the way, seats for the next workshops are already starting to disappear, so grab yours before it's too late!
Hair/Makeup: Cali Stott
Dress: Avenia Bridal
Flowers: Lizy's Lilies
Chair/Table: Big Day Vintage
Location: SLC Photo Collective


text:
I LOVE spring, it's so warm out, the flowers are blooming, and I love taking long walks with my little girl. Over the weekend I took her to farm day. A neighbor has a farm full of baby animals, she lets all the kids in the neighborhood come over to play. Brynlee loved it.
She loved chasing after the goats. I kept on telling her to be soft, but she would get so excited and squeal with delight and try to grab them.
| It was
so fun, we got to play with baby chicks, baby bunnies, and horses. I
can't wait for next year.
What's your favorite thing about spring?


text:
Halloween has always been one of my favorite holidays. Of course the candy had something to do with that when I was younger, and the parties at school, but nowadays it's more about coming up with a great costume and enjoying everyone else's at parties. I haven't always been the most inventive with costumes. Let's take it back a few years...
My mom sent me a couple of pictures of my brother and I in our Halloween costumes as kids. I'm not sure why they're in black and white... we definitely had color film when I was a kid... regardless, here we are, in front of the fireplace in our costumes. Mike was a vampire. I was a cat. Quite obvious, no? I mean what cat do you know that doesn't wear a bow tie with a black leotard and tights with bright orange pumpkins all over? (That lasted through multiple Halloween costumes by the way.) Although not pictured here, I definitely remember wearing some white velcro high tops. Probably LA Gear. I only wish it were in color so you could see that the carpet we're standing on was a classy shade of forest green.
Here we are again, sometime around the same year as the photo above, because there are those pumpkin tights again, and possibly the same high tops... And guess what! I was a pumpkin that year! So maybe a little more fitting for this costume. I'm not quite sure what's going on with the pumkin's expression or what's on my head, or what I'm holding... a ghost of some sort? All that really matters is the expression on pirate Mike's face. Priceless.
So I've skipped a few years, but I wanted to actually show you a costume that I'm proud of! This Halloween 2009. Sean went as Wall-E, and I went as his robot sidekick Eve. These costumes were handmade from head to toe, (well, minus Sean's belt and underclothes and our shoes) and we're so proud of them that they're still in our basement somewhere gathering dust :) This year is going to be a little bit different, but you'll have to stay tuned to find out what we're going to be. We're hosting our first Halloween party this evening, complete with a smoking cauldron, creepy music and candy corn jello shots. Happy Friday, and Happy Halloween!


text:
Heritage Category: Listed Building
Grade: I
List Entry Number: 1034494
Date first listed: 01-Oct-1953
Statutory Address: CHURCH OF ST THOMAS, ST THOMAS'S SQUARE
The above map is for quick reference purposes only and may not be to scale. For a copy of the full scale map, please see the attached PDF - 1034494 .pdf
The PDF will be generated from our live systems and may take a few minutes to download depending on how busy our servers are. We apologise for this delay.
This copy shows the entry on 23-Jan-2019 at 15:22:06.
Statutory Address: CHURCH OF ST THOMAS, ST THOMAS'S SQUARE
The building or site itself may lie within the boundary of more than one authority.
District: Isle of Wight (Unitary Authority)
Parish: Newport
National Grid Reference: SZ 49973 89145
Legacy Record - This information may be included in the List Entry Details.
Reasons for Designation
Legacy Record - This information may be included in the List Entry Details.
Legacy Record - This information may be included in the List Entry Details.
Parish Church, rebuilt 1854-5 by S W Dawkes of Cheltenham, in Decorated style retaining Pugin influence. Large and sited in the centre of the square with a prominent west tower. Rubble with ashlar dressings, the stone work is considerably weathered. Diagonal buttresses to tower and buttresses flanking aisles which have separate roofs and embrace the tower. Fine west door with good sculptural decoration to tall ogee arch. Good early Victorian interior which retains a number of very fine C17 furnishings. Font of 1636, small bowl with cover on very heavy large scrolls. Pulpit also of 1636 by the Fleming Thomas Caper, with upper panels representing the Seven Virtues, the bottom panels the Seven Liberal Arts bracketed bookrest, back panels and tester. The Reading Desk is made up of parts of the original Screen. Contemporary with the Pulpit and probably also by Caper. Alabaster monument to Sir Edward Horsey d 1582 recumbent effigy on a half-rolled-up map, (affinities with the later monument of Sir John Oglander in Brading Church IOW) strap work background, flanked by 2 columns, crowned by 3 achievements. Princess Elizabetg daughter of Charles I, who died in Carisbrooke Castle qv is buried here: white marble recumbent effigy by Baron Marochetti given by Queen Victoria in 1856. Memorial to Prince Albert with head in profile also by Marochetti. Above Chancel arch very elaborate Royal Arms of Queen Victoria decorated with flags.
Listing NGR: SZ4997589144
The contents of this record have been generated from a legacy data system.
Legacy System number: 309731
Legacy System: LBS
This building is listed under the Planning (Listed Buildings and Conservation Areas) Act 1990 as amended for its special architectural or historic interest.
End of official listing


text: I bought a new car that is bluetooth equipped. I followed the instructions for connecting my droid to the bluetooth and all is well. I tried to install my droid contact list to the cars database and am having trouble. The bluetooth screen say's to start the transfer but I don't know how to make the droid start transmitting the data. Can anyone help?


text:
Atlanta contracting distributor HD Supply Holdings Inc. said Wednesday it plans to purchase West Hartford's A.H. Harris Construction Supplies for $380 million.
Founded in 1916, A. H. Harris generated about $380 million in annual sales over the last 12 months, HD Supply said. The Connecticut company, which distributes building products and equipment to the heavy and highway, commercial, industrial and residential construction markets, has more than 600 employees and more than 50 locations in 12 states in the Northeast and mid-Atlantic.
The purchase strengthens that regional position for HD Supply and deepens its product expertise, said John Stegeman, president of HD Supply, which operates three companies in the U.S. and Canada. HD Supply has 220 locations in North America and more than 4,800 employees, the company said.
The deal is expected to close in HD Supply's first fiscal quarter of 2018.
Editor's Note: This story was corrected as Kim Corwin is no longer CEO of A.H. Harris.
Sign up now for our daily and weekly
e-newsletters! Click Here


text:
Cracker Barrel Old Country Store's top shareholder, Sardar Biglari, said he was considering a bid for the restaurant chain, marking the activist investor's latest attempt to take control of the company.
Sardar Biglari, through his investment firm Biglari Holdings, has been pushing for change at the owner of Steak 'n Shake and Western Sizzlin restaurants for more than two years, saying the company's "earning power" was far too low under the current management.
Biglari Holdings, which holds a 19.9 percent stake in Cracker Barrel, said on Tuesday it was in talks with an investment bank to finance a deal
(Read more: McDonald's employee site bashes fast food)
Biglari also said he would consider selling his stake if the company began a share repurchase.
"We would consider selling our entire position because we would not want to leave our money in your care. The handling of the 'Duck Dynasty' controversy is another example of poor judgment," he wrote.
After the chain's removal of goods related to the reality-television show "Duck Dynasty" from its locations because one of the cast's anti-gay comments, a fan outcry ensued. The chain relented and restocked the shelves.
Cracker Barrel's shares rose 1 percent in early trading.
Sardar Biglari's latest attempt in November to win board seats was rebuffed by Cracker Barrel's shareholders at the annual meeting. They also rejected his proposal for a $20 per share special dividend.
Biglari last week sent a letter to the Cracker Barrel's board, urging a review of options including a sale.
(Read more: Eat, drink and bemerry—but is the food safe?)
In Tuesday's letter, Biglari said he was willing to lead a sale process by submitting a bid, but did not give details.


text: Sorry if this topic has been covered elsewhere, I've searched hard & found nothing. How can one manage contacts within the phone. Somehow my phone has 4 "favorites" but can't figure out how to edit, add or delete. For managing contacts from a PC everything seems aimed at sync'ing everything and that is what I DON'T Want, is there some app that could allow me to manage the short list of contacts I want on my phone? Thanks.


text:
Vuzix Schedules Conference Call to Discuss Second Quarter 2015 Financial ResultsPress Releases Follow |
ROCHESTER, N.Y., Aug. 5, 2015 /PRNewswire/ -- Vuzix® Corporation (VUZI) , a leading supplier of Video Eyewear and Smart Glasses products in the consumer, enterprise, and entertainment markets, is pleased to announce that the Company has scheduled a conference call for Friday, August 14, 2015 at 8:30 AM Eastern Time (ET) to review its operating results for its fiscal quarter ended June 30, 2015.
Date: Friday, August 14, 2015
Time: 8:30 AM Eastern Time (ET)
Dial-in Number for U.S. & Canadian Callers: 877-407-0839
Dial-in Number for International Callers (Outside of the U.S. & Canada): 201-689-8863
Participating on the call will be Vuzix Chief Executive Officer and President Paul Travers and Chief Financial Officer Grant Russell, who will discuss operational and financial highlights for the second quarter 2015.
To join the live conference call, please dial in to the above referenced telephone numbers five to ten minutes prior to the scheduled call time. A live webcast and archive of the call will also be available on the Vuzix website at: http://vuzix.equisolvewebcast.com/fr-8-14.
A replay will be available for 14 days starting on August 14, 2015, at approximately 11:00 AM (ET). To access the replay, please dial 877-660-6853 in the U.S. and 201-612-7415 for international callers. The conference ID# is 13595774.
About Vuzix Corporation
Vuzix is a leading supplier of Video Eyewear and Smart Glasses products in the consumer, enterprise and entertainment markets. The Company's products include personal display and wearable computing devices that offer users a portable high quality viewing experience, provide solutions for mobility, wearable displays and virtual and augmented reality. Vuzix holds 41 patents and 10 additional patents pending and numerous IP licenses in the Video Eyewear field. The Company has won Consumer Electronics Show (or CES) awards for innovation for the years 2005 to 2014 and several wireless technology innovation awards among others. Founded in 1997, Vuzix is a public company (NASDAQ: VUZI) with offices in Rochester, NY, Oxford, UK and Tokyo, Japan.
Forward-Looking Statements Disclaimer
Certain statements contained in this news release are "forward-looking statements" within the meaning of the Securities Litigation Reform Act of 1995 and applicable Canadian securities laws. Forward looking statements contained in this release relate to, among other things, and the Company's leadership in the Video Eyewear, VR and AR display industry. They are generally identified by words such as "believes," "may," "expects," "anticipates," "should" and similar expressions. Readers should not place undue reliance on such forward-looking statements, which are based upon the Company's beliefs and assumptions as of the date of this release. The Company's actual results could differ materially due to risk factors and other items described in more detail in the "Risk Factors" section of the Company's Annual Reports and MD&A filed with the United States Securities and Exchange Commission and applicable Canadian securities regulators (copies of which may be obtained at www.sedar.com or www.sec.gov). Subsequent events and developments may cause these forward-looking statements to change. The Company specifically disclaims any obligation or intention to update or revise these forward-looking statements as a result of changed events or circumstances that occur after the date of this release, except as required by applicable law.
Investor and Media Relations Contact:
Andrew Haag
Managing Partner
IRTH Communications
Vuzix Corporation
2166 Brighton Henrietta Townline Road
Rochester, NY 14623 USA
Investor Information – Grant Russell
Tel: (585) 359-7562
DISCLOSURE: The views and opinions expressed in this article are those of the authors, and do not represent the views of equities.com. Readers should not consider statements made by the author as formal recommendations and should consult their financial advisor before making any investment decisions. To read our full disclosure, please go to: http://www.equities.com/disclaimer
|Vuzix Corporation


text:
About this font family
Cacao is another one of my "found fonts". I found this one in an old advertising for a French cocoa drink.
Since I am a vervent lover of cocoa, I will give you my recipe for a normal coffee mug full of delicious hot cocoa. Mix three heaped teaspoons of sugar with one and a half to two teaspoons of finest cocoa powder. Then add a little cold milk, stir, add a little cold milk, stir, and so on until you have a mushy creamy consistency. Now slowly add - always stirring - boiling hot water til the cup is almost full. Top with a little liquid cream and enjoy! More…
If you have a package design job, use my Cacao font and stir in some creativity.
Your sweet-tooth designer, Gert Wiescher.
- Basic text strings
- Random text strings
- News feeds
- Pangrams
Cacao Plain
Cacao Plain Outline
Cacao Plain Inline
Cacao Plain Swashes
Cacao Fat
Cacao Fat Outline
Cacao Fat Inline
Cacao Fat Swashes
Available formats
available in all of the styles:


text:
NFL Hall of Famer Dick Butkus DID WHAT On The Set of 'I Didn't Do It'?
Olivia Holt and Austin North find themselves among football legends in this behind-the-scenes pic from I Didn't Do It.
In this weekend's episode "Ball or Nothing," when Lindy (Holt) and Logan (North) accidentally destroy their father's football signed by NFL Hall of Famer Dick Butkus, they go on a mission to track down the Chicago football legend and have him sign a new ball.
Plus, we spy Jasmine (Piper Curda) in less than her fashionable garbs — what is going on there?!
"Ball or Nothing" airs TOMORROW, Sunday, August 24th @ 8:30PM ET/PT on Disney Channel. 20+ pics inside…
Check out an exclusive featurette from taping, too!
"I Didn't Do It" — "Ball Or Nothing" Featurette
Posted to: Austin North, I Didn't Do It, Olivia Holt, Peyton Clark, Piper Curda, Sarah Gilman


text:
|First Hexie Bee block.
|Only have 2 strips of my Foxy Scout quilt together. Only 8 to go.
|One of the bee blocks I'm working on.
I also have some exciting news! I've been accepted in to do. Good stitches. It is a modern charity bee. Someone had to drop out of a group and I am taking their place. My new group donates the quilts they make to project Linus. I am so excited to be able to help others with my new passion.
And later today I am going to a friends house to help her with her first quilt. I'm happy to be helping her, but it is kind of weird to tell her what to do since she is nearly 10 years older than me.
Finally this weekend my husband got a new car. He had been driving around a 1995 Mercury that he bought for $500 5 years ago. It had no ac, someone has knocked off the drivers side mirror, and it had manual locks and windows. I let him pick whatever he wanted and he bought a brand new Altima with all the bells and whistles-even a moon roof and nav system. I'm glad he finally got something for himself. He has been so supportive of everything I've done (and lenient on my spending habits!). How do your loved ones appreciate you?


text:
For Immediate Release
Dan Beeton, 202-239-1460
MINUSTAH Must Take Responsibility for Causing Cholera Epidemic That's Killed 7,000 People, CEPR Co-Director Says
Statements by UN Spokesman Nigel Fisher "False and Deceptive"
WASHINGTON - The United Nations Stabilization Mission in Haiti (MINUSTAH) owes it to the half-a-million cholera victims in Haiti to take responsibility for having caused the outbreak, Center for Economic and Policy Research (CEPR) Co-Director Mark Weisbrot said today. Weisbrot also called recent statements by UN's head of Humanitarian Affairs in Haiti, Nigel Fisher, regarding the origins of the epidemic "false and deceptive."
"It is deplorable that a full 14 months after the discovery of the first cholera case, the UN continues to dodge responsibility for having reintroduced a deadly disease that has now killed a minimum of 7,000 people," Weisbrot said. "This is a case of criminal negligence, and the UN, if it is to continue to be worthy of the respect of people around the world, must own up to the fact that it caused this problem.
"Even worse, the UN, through its spokespeople, is attempting to mislead the public about the type of cholera strain that has been identified in Haiti."
Weisbrot noted that in recent comments to the BBC, Fisher claimed that "The cholera strain we have in Haiti is the same as the one they have in Latin America and Africa. They all derive from Bangladesh in the 1960s so they are all an Asian strain."
But this directly contradicts several scientific studies. Even the UN's own report states: "Overall, this basic bacteriological information indicates the Haitian isolates were similar to the Vibrio cholerae strains currently circulating in South Asia and parts of Africa, and not to strains isolated in the Gulf of Mexico, those found in other parts of Latin America…" and that "A careful analysis of the MLVA results and the ctxB gene indicated that the strains isolated in Haiti and Nepal during 2009 were a perfect match."
In a case that continues to attract international interest and attention, last month, lawyers with the Institute for Justice and Democracy in Haiti and the Bureau des Avocats Internationaux filed for damages from the UN on behalf of 5,000 cholera victims. A Brazilian organization, the Faculdade de Direito de Santa Maria, has also filed a separate petition [PDF], seeking intervention by the Inter-American Commission on Human Rights, and calling for the UN to provide compensation to Haiti, the Dominican Republic, and other affected countries, and for a fund of at least $500,000,000 to create a public health system in Haiti.
This is the world we live in. This is the world we cover.
Because of people like you, another world is possible. There are many battles to be won, but we will battle them together—all of us. Common Dreams is not your normal news site. We don't survive on clicks. We don't want advertising dollars. We want the world to be a better place. But we can't do it alone. It doesn't work that way. We need you. If you can help today—because every gift of every size matters—please do.
The Center for Economic and Policy Research (CEPR) was established in 1999 to promote democratic debate on the most important economic and social issues that affect people's lives. In order for citizens to effectively exercise their voices in a democracy, they should be informed about the problems and choices that they face. CEPR is committed to presenting issues in an accurate and understandable manner, so that the public is better prepared to choose among the various policy options.


text:
Pool Heaters Gas Lines
Installing a natural gas pool heater is the best choice you can make for efficiency, saving you money and pool comfort as they are quite effective for heating. If you're looking to get a gas line for your pool or hot tub heater, you'll appreciate the friendly, professional service and great value you receive from Some Like It Hot.
We are also trained and experienced in troubleshooting Natural Gas pool heater problems and can complete the necessary pool heater repairs.


text:
This week, I started drafting Daisy Chain!
I'm only on Chapter 2, but that's because of a combination of real life, the outlining method, and now, the fact that I'm trying to roughly structure where my characters go in terms of museums. I'm hoping that getting that out of the way now will make it make more sense and make it easier for me to work out where to go if I see an opportunity for on-location research, especially with the job I have – the fact that I work half-day on Friday and it's not in the office all the time.
The things I'm most excited about? Figuring out ways to add history references and kissing. So much kissing!


text:
you know you've got to force yourself to sleep
when you start watching the mint/quizmania at 2 am.
stupid heat.
the real problem is when you start ringing. My girlfriend wasn't happy when the phonebill came in...
I'm bored complaining about the heat
We'll all be complaining about the cold in 6 months anyway.
That is really depressing. I always have nights like that. Its almost more satisfying when you get to sleep though, so i quite like it.
that quizmania thing
is quite hilarious. I'd love to see the proof that anyone has actually won anything.
I hate the show
but I see the quiz or puzzle or whatever and I think I have the answer, so then I feel like I have to watch and wait and see if someone calls in with my answer and see if I'm right, stupid program!!
what I find sends me to sleep
Is a really mind-blowing, draining album. We've all got a few of those. Put that on your ipod or something. It ALWAYS works.
I can't sleep if there's music playing, ever. But thank you.
but then
you've got to pull your ear plugs out at the end. And the action wakes me up again.
I rarely fall asleep
when the music is playing. But I'm shattered when it's done.


text:
20 Years of Investing in a Stronger, More Inclusive New York
Two Decades of Accomplishments by the $160 million Civic FundRead More
Economic Indicators: Q3 2018 Dashboard | Total Jobs Up 1.7% | Unemployment Rate Down 0.5%
Global Business, Local Benefit
Foreign Contributions to the New York EconomyRead More
Total VC Investment Up 34.4% | VC Investment in Tech Up 126.3%
Clear for Takeoff
A Better Future for New York Metro AirportsRead More
Reported Non-Violent Crimes Up 2.7% | Reported Violent Crimes Down 2.1%
Commercial Life Sciences Can be New York's Next Big Industry
A New Report from The Partnership FundRead More
City Spending Down 0.7% | Property Tax Revenue Up 4.3%
We are New York City's Business Leadership
Read More
We are New York City's business leadership.
The Partnership for New York City represents the city's business leadership and its largest private sector employers. We work with government, labor and the nonprofit sector to promote economic growth and maintain the city's position as a global center of commerce and innovation. Through the Partnership Fund for New York City, the Partnership contributes directly to projects that create jobs, improve economically distressed communities and stimulate new business creation.
Statement on Governor Cuomo's State of the State Address from the Partnership for New York CityRead More


text:
Green Energy Environmentally Friendly Building Google Map Launched
Green Energy Using Green Building Google Map Launched.
Exposing "Brown Buildings" and environmentally friendly "Green Buildings" alike, You can finally decide to do business with a business based on it's environmental savvy. Apartments, Offices, Retail Stores, and Factories are featured. You can rank each of these buildings Black, Brown, or Green based their eco friendly energy usage.
"Let's put Green Back on the Map!tm"


text:
1. Trace patterns (available, below) onto tracing paper and cut out.
2. Using an iron on medium heat, adhere freezer paper, shiny side down, to each color of felt.
3. Referring to photo, trace pattern shapes on respective felt colors.
4. Cut out shapes, using pinking shears to cut bottom edge of each tree branch.
5. Peel off freezer-paper.
6. Glue tree shapes to red oval.
7. Glue a hanging loop of baker's twine to the back of the red oval.
8. Glue ornament to aqua felt, enclosing ends of hanging loop, and cut around shape using pinking shears.
Be the first to comment!


text:
There are plenty of things you can do to get your car ready for winter driving. Get new windshield wiper blades. Refill your wiper fluid. Make sure your 4-wheel drive is working properly. Check your battery. Change your oil. Performing each of these tasks is a good idea if you want to ensure that your vehicle is in tip-top shape for the coming winter season. But while these things are all important, getting suitable tires for your Massillon, Ohio or North Canton, Ohio vehicle should be your first priority. Not sure that you can afford new wheels right now? Well, Meineke Car Care Center of Canton can make it a lot easier on your wallet. This Canton, Ohio auto repair company offers affordable tires throughout the year, but right now you can save on tough tires for winter by filling out a brief survey. By taking just twenty seconds out of your day to answer a few questions, you can get a car service coupon from Meineke Car Care Center to help pay for some much needed vehicle maintenance or repair.
Now, you may wonder how important it is to have brand new tires on your vehicle for winter driving. After all, if you have 4-wheel drive, that should be good enough, right? Not exactly. While having power from all sides can be helpful, it's no substitute for having a set of quality tires on your Massillon or North Canton car. You need to have wheels made of thick, strong rubber for maximum performance during the winter months so you have proper traction on the roads. And when you contact Meineke Car Care Center of Canton, that's exactly what you'll receive.
Meineke Car Care Center has been a trusted name in vehicle repair and maintenance for over fifty years. And since this company is one that truly cares about its customers' input, they've developed a brief survey for people to fill out for a car service coupon. By visiting our feedback survey and giving less than a minute of your time to answer some simple questions, you can receive cash off of Meineke's already affordable tires. The coupons are geared towards your answers, so you have the opportunity to get $30 off two tires, $75 off four tires, an oil change for $16.95, or a coolant flush & fill for $69.95. Meineke Car Care Center knows how slick and icy the roads in Northeast Ohio can be, so they want to help you save on tough tires that can better maneuver through the slush and snow of winter.
If your North Canton, Ohio or Massillon, Ohio car could use some preparation for winter driving, your best bet is Meineke Car Care Center of Canton, Ohio. Not only can you have the trained technicians perform routine maintenance on your vehicle, but you can purchase some of their affordable tires as well. Just don't forget to visit our feedback survey to fill out the brief survey so you can receive a car service coupon to help you save on tough tires. For more information about Meineke Car Care Center and the services they offer, visit www.Meineke.com or give them a call at 330.477.2236.


text:
WM Splitter 2.2.1409.56 | 16 Mb
WM Splitter is a small and easy to use application that will enable you to trim and even join together audio and video files. Simply load a media file into the program and place markers along the timeline where you want to exclude, or keep, a potion of the file. WM Splitter allows you to mark your sections using a variety of navigation tools that give you precise control, including frame-by-frame, time based, and even K-Frames. Once you have finished marking the section you simply press the "trim" button and your edited file is saved, along side your original file. WM Splitter has a "join manager" that allows you to easily join files of the same file type. It's super fast and easy.
WM Splitter can edit these popular video and audio formats: AVI, WMV, ASF, WMA and MP3. The program is super fast. You can remove commercials from all your favorite videos or trim dead air away from all your favorite MP3's. Use this program to get all your favorite files in exactly the shape you want them. Even a computer novice will have no trouble figuring out how to use this program.
Download WM Splitter 2.2.1409.56
2018, full version, warez, serial numbers, cracks, keygen, serials, nulled, cracked, patch, key, activator, 2015


text:
Hi Everyone,
As I mentioned last week, the Halloween Cardmaking season is now in full swing at my house. Seriously, the 2 Cute Ink Halloween and Fall digi stamps are amazingly fun to work with! With cute Halloween/Fall spiders and pumpkins and bats and cats, these digi stamps will make your cards pop!
For today's card, I used the Pumpkin/Leaves digi stamp....and loved how this one turned out in glitter (especially the leaves). I popped the pumpkin up with a wobble sticker. So my pumpkin is an "action" raker. Instructions for using glitter with a digi stamp can be found here.
Here's my pumpkin "wobbling."
Supplies Used
- Pumpkin Leaves Digi Stamp
- Glitter
- Copper, Java Bean, Classic Taupe, Regal Red (Glitter Ritz)
- Cardstock - Halloween paper pack purchased on clearance at Hobby Lobby
- Action Wobble


text:
Transdifferentiation is a driving force of regeneration in Halisarca dujardini (Demospongiae, Porifera)
- Published
- Accepted
- Received
- Academic Editor
- Linda Holland
- Subject Areas
- Developmental Biology
- Keywords
- Sponges, Regeneration, Morphogenesis, Epithelial-to-mesenchymal transition, Transdifferentiation, Halisarca dujardini
- Copyright
- © 2015 Borisenko et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
- Cite this article
- 2015. Transdifferentiation is a driving force of regeneration in Halisarca dujardini (Demospongiae, Porifera) PeerJ 3:e1211 https://doi.org/10.7717/peerj.1211
The ability to regenerate is widespread in the animal kingdom, but the regenerative capacities and mechanisms vary widely. To understand the evolutionary history of the diverse regeneration mechanisms, the regeneration processes must be studied in early-evolved metazoans in addition to the traditional bilaterian and cnidarian models. For this purpose, we have combined several microscopy techniques to study mechanisms of regeneration in the demosponge Halisarca dujardini. The objectives of this work are to detect the cells and morphogenetic processes involved in Halisarca regeneration. We show that in Halisarca there are three main sources of the new exopinacoderm during regeneration: choanocytes, archaeocytes and (rarely) endopinacocytes. Here we show that epithelial-to-mesenchymal transition (EMT) and mesenchymal-to-epithelial transition (MET) occur during Halisarca regeneration. EMT is the principal mechanism during the first stages of regeneration, soon after the injury. Epithelial cells from damaged and adjacent intact choanocyte chambers and aquiferous canals assume mesenchymal phenotype and migrate into the mesohyl. Together with archaeocytes, these cells form an undifferentiated cell mass beneath of wound, which we refer to as a blastema. After the blastema is formed, MET becomes the principal mechanism of regeneration. Altogether, we demonstrate that regeneration in demosponges involves a variety of processes utilized during regeneration in other animals (e.g., cell migration, dedifferentiation, blastema formation) and points to the particular importance of transdifferentiation in this process. Further studies will be needed to uncover the molecular mechanisms governing regeneration in sponges.
Regeneration is a biological phenomenon which is critical for the survival of an organism. Reparative regeneration is widely distributed among metazoans, with both similar and divergent cellular and morphogenetic mechanisms utilized within and among different taxa (Sànchez Alvarado, 2000; Tanaka & Reddien, 2011; Vervoort, 2011). To understand the evolutionary history of the diverse regeneration mechanisms, the regeneration processes must be studied in early-evolved metazoans in addition to the traditional bilaterian and cnidarian models.
The regeneration of injured or lost body parts is particularly common in epifauna, and is essential for community recovery from external disturbances that damage these animals (Henry & Hart, 2005). Sponges (phylum Porifera) are benthic sedentary animals which are not equipped with protective tissues or structures like cuticles, scales or shells, and are covered only by a single-cell layer (exopinacoderm). It has been suggested that this lack of protection against injury closely correlates with the high regenerative capacity of sponges (Korotkova, 1997). However, the patterns and rate of regeneration are strikingly different among different sponge species (Henry & Hart, 2005; Wulff, 2013). The regenerative capacities of sponges range from an ability to restore a lost body part, to development from a piece of tissue, or restoration of a complete organism from an aggregate of dissociated cells (Korotkova, 1997).
A large number of studies on regeneration in Porifera, carried out in the last decade, were performed on somatic cell conglomerates called primmorphs (for review see: Nickel & Brümmer, 2003; Chernogor et al., 2011; Lavrov & Kosevich, 2014; Eerkes-Medrano, Feehan & Leys, 2015). Primmorphs represent a suitable model to study de-differentiation or differentiation of cells, as these events do take place during primmorph development. However, as an artificial system primmorphs are not an ideal model for the study of reparative regeneration in whole organisms or its parts. In spite of the importance of sponges as excellent models for investigation of the evolutionary history of regeneration mechanisms and morphogenesis, studies of regeneration after surgical injury or development of sponges from body fragments have been generally limited to histological descriptions using light microscopy (Korotkova, 1961; Korotkova, 1963; Korotkova, 1970; Korotkova, 1972; Korotkova & Nikitin, 1969a; Korotkova & Nikitin, 1969b; Diaz, 1979). There are only a few ultrastructural studies, describing regeneration of sponge structures such as oscular diaphragm (Thiney, 1972) or inhalant papillae (Boury-Esnault, 1976). It was shown that in many sponges, regeneration and primmorph formation from dissociated cells is accompanied by cell transdifferentiation (reviewed in: Simpson, 1984; Korotkova, 1997). Transdifferentiation is the conversion of one type of already differentiated cell to another type of normal differentiated cell. In some cases, transdifferentiation is accompanied by cell division, whereas in others it is not (Tosh & Slack, 2002; Shen, Burke & Tosh, 2004).
Halisarca dujardini Johnston, 1842 (class Demospongiae) is a convenient sponge for studying regeneration. It is a common species in littoral habitats along the European coasts from the English Channel to the White Sea (Ereskovsky et al., 2011), and is accessible throughout the year. Absence of spicules and skeleton makes this sponge useful for histological processing. Embryonic development, metamorphosis and morphology of H. dujardini have been described in detail (Lévi, 1956; Ereskovsky & Gonobobleva, 2000; Ereskovsky, 2002; Gonobobleva & Ereskovsky, 2004a; Gonobobleva & Ereskovsky, 2004b; Mukhina et al., 2006; Gonobobleva & Maldonado, 2009; Ereskovsky, Konjukov & Willenz, 2007; Ereskovsky et al., 2011).
It has been previously demonstrated that H. dujardini has high regenerative capacity. A study on the cellular response to the introduction of foreign material into the sponge body has shown that the sponge response combines protective and regenerative processes (Korotkova & Movchan, 1973). Transdifferentiation of choanocytes into exopinacocytes and endopinacocytes has been shown to occur during regeneration of the sponge from conglomerates of dissociated cells (Volkova & Zolotoreva, 1981). Finally, regeneration of H. dujardini from small body fragments has been described at the light microscopy level (Korotkova, Suchodolskaya & Krasukevitch, 1983; Sukhodolskaya & Krasyukevich, 1984). This type of injury leads to the restructuring of most of the aquiferous system. Labeling choanocytes by China ink suspension, the authors demonstrated a variety of fates of choanocytes: a significant proportion remains in the mesohyl as dedifferentiated cells, others are included in the exopinacoderm, and a few are transdifferentiated into endopinacocytes of the aquiferous canals. Epithelialization of the wound surfaces was suggested to be due to the stretching of the adjacent exopinacoderm, and also differentiation of archaeocytes (Korotkova, Suchodolskaya & Krasukevitch, 1983; Sukhodolskaya & Krasyukevich, 1984).
In the current work, we revisited the regeneration of Halisarca dujardini using transmission and scanning electron microscopy, allowing us to more precisely address the issues of cell transdifferentiation and movement. In addition, we have studied cell proliferation during regeneration in this species. Our study demonstrates a variety of morphogenetic processes during reparative regeneration, and identifies cells involved in these processes.
Material and Methods
Sponge materials
Sponges Halisarca dujardini Johnston (Demospongiae, Chondrosida) were collected in the White Sea (Chupa Inlet, Kandalaksha Bay) in June–July 2010–2012 and in April of 2010 and March of 2011 near island Sotra in the North Sea (Bergen, Norway). Sponges were collected with their algal substrate and maintained in aquariums with sea water at +12 °C in the Marine Station of the Zoological Institute RAS (Kartesh, White Sea) and in the Sars International Center for Marine Molecular Biology (Bergen, Norway).
Field study permissions
No specific permissions were required for these locations because the study was done outside of the national park, private land or protected area. We confirm that the field studies did not involve endangered or protected species.
Surgical procedures
Manual dissections were performed with the aid of a stereomicroscope and use of Castroviejo scissors and microscalpels. For each experiment, a portion of apical ectosome (superficial part of the sponge) was removed, along with a directly underlying section of the aquiferous system (choanocyte chambers and canals). The depth of excision varied slightly between the operations, from 150 to 500 µm. The osculum (exhalant opening) remained intact in all cases. Wounded sponges were maintained in 40 mm Petri dishes with 0.22 µm-filtered sea water replaced daily. Six individuals were observed for each time point at various intervals until regeneration was complete. Timing was started from 0 h (wounding); regeneration was monitored under a dissecting microscope and specimens were fixed at 3, 6, 12, 24, 48 and 72 h after excision.
Light and electron microscopy
For microscopic investigation sponges were fixed in a solution composed of one volume of 25% glutaraldehyde, four volumes of 0.2 M cacodylate buffer and five volumes of filtered seawater for 2 h and post-fixed in 2% OsO4 in seawater at room temperature for 2 h. After fixation, samples were washed in 0.2 M cacodylate buffer and distilled water successively, and finally dehydrated through a graded ethanol series. For semi thin sections and transmission electron microscopy (TEM) specimens were embedded in Araldite resin. Semi thin sections (1 µm in thickness) were cut on a Reichert Jung ultramicrotome equipped with a diamond knife "Micro Star" 45°, then stained with toluidine blue, and observed in a WILD M20 microscope. The digital photos were made on a Leica microscope DMLB with the system of photo capture Evolution LC color. Ultrathin sections (60–80 nm) were cut with a Leica UCT ultramicrotome equipped with a diamond knife Drukkert 45°. Ultrathin sections, contrasted with uranyl acetate, were observed under a Zeiss-1000 transmission electron microscope (TEM). For scanning electron microscopy (SEM), fixed specimens were critical-point-dried, sputter-coated with gold-palladium, and observed under a Hitachi S 570 SEM.
Cell proliferation investigation
For labeling of newly synthesized DNA we used 5-Ethynyl-2′-deoxyuridine (EdU; Molecular Probes), which is incorporated into genomic DNA during S-phase (Salic & Mitchison, 2008). A series of experiments with different concentrations of EdU and incubation times was performed to establish optimal conditions of labeling. In most cases, incubation time between addition of EdU and fixation was 6 h although images with longer incubation are also presented. EdU was added 6 h before the end of the experiment; thus, in the case of a 6 h of regeneration, EdU was added immediately after surgery, and in experiments with 12 h of regeneration—6 h after surgery and 6 h before fixation. This experimental setup resulted in labeling of DNA synthesized during the last 6 h of regeneration in each experiment. Stock solution of EdU in DMSO (500 mM) was added to filtered sea water with sponges to a final concentration of 800 µM. Six sponges were used for each stage of regeneration. Non-operated sponges, placed at the same time in the seawater with EdU as the operated experimental sponges, served as experimental controls. Sponges cultivated in sea water without EdU served as negative technical controls.
Specimens at different stages of regeneration and immediately after wounding were fixed in 4% paraformaldehyde in PBS (phosphate buffered saline with pH 7.4) for 1 h followed by washing in PBS. Fixed and rinsed specimens were blocked in 5% normal sheep serum/0.05% Tween-20 in PBS during 1 h at room temperature. Specimens were treated with the Click-iT® EdU Alexa Fluor® 488 Imaging Kit (Molecular Probes, Carlsbad, California, USA) according to manufacturer instructions, and then incubated in mouse anti-tubulin antibody (Sigma-Aldrich, Seelze, Germany) at 4 °C overnight. Specimens were then rinsed three times in blocking buffer during 3–5 h at while rotating and incubated in Alexa Fluor® labeled anti-mouse secondary antibody (Molecular Probes, Carlsbad, California, USA). After 12 h of incubation in the secondary antibody, specimens were rinsed and the DNA was stained with TO-PRO® -3 (Molecular Probes, Carlsbad, California, USA) at concentration 1 µM in PBS during 2 h. Rinsed samples were mounted in DABCO-glycerol and images obtained using a multiphoton confocal microscope Leica TCS SP5 MP with white laser WLL. Pictures processed with LAS AF Lite (Leica Microsystems, Wetzlar, Germany) and ImageJ software (http://imagej.nih.gov/ij/).
Anatomy and cytology of Halisarca dujardini
Halisarca dujardini is a predominantly shallow water, encrusting sponge growing up to 0.5–4 cm in diameter with thickness of 0.5–12 mm (Fig. 1A). Inorganic and organic skeletons are absent, and the surface is smooth. The body is composed of the peripheral ectosome and the internal choanosome (Figs. 1B and 1C; see also cartoon representation on Fig. 2). The ectosomal region is up to 27 µm thick and consists of three layers: (1) external parts of T-shaped exopinacocytes (Fig. 1D) which are covered an acellular mucous cuticle; (2) layer containing collagen fibrils and rare spherulous cells; and (3) the inner layer, consisting of condensed collagen fibrils and the cell bodies of exopinacocytes. The choanosome (Fig. 1B) makes up the greatest volume of the sponge body and is composed of choanocyte chambers (built of choanocytes the only cell type which is flagellated, Fig. 1C), aquiferous canals (built of endopinacocytes, Fig. 1E) and the mesohyl. Populations of free cells in the mesohyl of H. dujardini include: archaeocytes, lophocytes, spherulous cells, granular cells, microgranular cells, and vacuolar cells (Ereskovsky et al., 2011) (Figs. 1E–1H). Neither specialized cell junctions, nor basement membranes could be identified in association with any of the cell types.
Description of regeneration
The observed regeneration processes in Halisarca dujardini can be subdivided into three stages: I—formation of "regenerative plug" (0–12 h), II—wound healing and formation of a "blastema" (12–36 h), and III—restoration of ectosome and choanosome (36–72 h).
Stage I—formation of a "regenerative plug"
Sections of ectosome together with the directly adjacent choanosome were surgically removed at the beginning of the experiment (Fig. 2). Immediately after the injury, the wound surface retracts, leaving the surface of the intact ectosome protruding around the edges of the wound (Figs. 3A and 3B). The ectosome and the upper areas of choanosome are destroyed (Fig. 3C). However, the structure of the aquiferous system in the deeper zone of choanosome is preserved. The wound surface is covered with exudate and cell debris. A number of amoeboid cells can be identified among the extracellular matrix fibers (ECM) (Figs. 3A–3D). Choanocyte chambers and aquiferous canals in the damaged zone disintegrate: cells of these structures lose contacts with adjacent cells and change their shape from trapeziform (choanocytes) and flat (endopinacocytes) to spherical or amoeboid (Figs. 3E–3G). These changes mark the beginning of dedifferentiation (followed by transdifferentiation, see next section), which is accompanied by migration of the cells into the mesohyl. The de- and trans-differentiating cells can be tracked thanks to preservation of their characteristic organelles. The natural label of dedifferentiated choanocytes is the flagellar apparatus (basal body and accessory centriole situated next to the nucleus), which remains in the cell. The collar of microvilli is reduced and disappears, and the flagellum resorbs (Fig. 3E), although some dedifferentiated choanocytes keep their flagella up to the last stage of transdifferentiation into an exopinacocyte (see: Fig. 9H). Endopinacocytes, which are flat in their intact state, assume spherical to amoeboid shapes and can be identified by their small anucleolated nuclei.
During the initial six hours after injury, the "regenerative plug" forms at the wound area. It consists of exudate (mucus), bacteria and cell debris, and includes dedifferentiated pinacocytes, choanocytes, spherulous and granular cells of the mesohyl (Figs. 3A and 3D). In the wound area, vacuolar cells, phagocytes and archaeocytes can also be found. With exception of the spherulous cells, all cells are filled with phagosomes, testifying to their involvement in active phagocytosis of the cell debris (Figs. 3E–3G).
At 12 h after injury, the wound surface is completely covered with exudate and ECM (Figs. 4A and 4B), with minor amount of debris still detectable at the surface. Underlying choanosome areas are disorganized (Figs. 4C–4F). Intact exopinacocytes, surrounding the wound, change the shape of their flat cytoplasmic outgrowths, and the peripheral parts of adjacent cells become separated (Fig. 4G). Reorganization of the ectosome begins with restoration of its middle layer containing interlaced collagen fibrils organized into firm tracts (Fig. 4A).
Stage II—wound healing and blastema formation
24 h after wounding, the ectosome is composed of a well-developed dense collagen layer, which is comparable to that of the intact ectosome (Fig. 5A compare with Figs. 8A and 8B).
Archaeocytes, which are toti- or pluripotent cells of demosponges (Funayama, 2013), and the dedifferentiated cells (choanocytes, pinacocytes) accumulate beneath the wound surface (Fig. 5B). This accumulation of cells strikingly resembles blastemas formed during regeneration of other animals. In line with this similarity, the new exopinacoderm (ectosome) and aquiferous system (choanosome) structures form from the cells of the blastema.
Differentiation of exopinacocytes is individual, and begins with cells migrating from the blastema towards the wound surface. Upon reaching the wound, the cells flatten, assuming positions parallel to the surface (Figs. 5C and 5D). Ultrastructural characters demonstrate that the migrating cells include archaeocytes, dedifferentiated choanocytes (Figs. 5E and 5F), endopinacocytes and exopinacocytes and newer other mesohyl cells types. Then, these cells begin to transform into T-shaped exopinacocytes, characteristic of the adult sponge: most of the cytoplasm of the cell and the nucleus are inside the ectosome. A fine cytoplasmic bridge is retained between the apical plate and the immersed part of the cell (Fig. 6). The apical parts of new exopinacocyte extend into large polygonal plates.
At 24 h after injury, the part of choanosome which is underlying the wound remains anarchized, presenting as an accumulation of individual cells rather than continuous epithelium (Fig. 5G). These cells include many phagosomes (Fig. 5H); some cell debris remains visible in the area.
Stage III—restoration of ectosome and choanosome
At 48 h after injury, many newly differentiated exopinacocytes are present at the wound surface, but a continuous exopinacoderm has not yet formed (Fig. 7A). Numerous small cytoplasm microvilli develop at the apical surface of the newly formed exopinacocytes, showing that these cells are motile (Fig. 7B). Cell bodies of these differentiated exopinacocytes are concentrated in the middle layer of the ectosome (Figs. 7C and 7D).
During regeneration of the choanosome, the aquiferous canals and choanocyte chambers form by association of previously disaggregated endopinacocytes and choanocytes, respectively (Figs. 7D and 7E). Cells of each type contact each other and connect by interdigitations (Fig. 7F). Individual choanocytes form groups of cells, forming structures less compact than in the intact choanocyte chamber (Figs. 7G and 7H). Some mesohyl cells still include phagosomes.
Scar-free wound healing is complete approximately 72 h after the injury (Fig. 8). At this point, the new exopinacoderm is identical to the intact one (Figs. 8A and 8B). External flat cytoplasmic parts of exopinacocytes connect with each other by interdigitations. Their apical surfaces are covered with a glycocalyx. All mesohyl cells, as well as the choanocytes and endopinacocytes, are free of the phagosomes and have morphology typical of the intact area (Fig. 8D).
Cell proliferation during regeneration
Cells of intact sponges actively incorporate EdU, and after 6 h, labeling nuclei of numerous cells—mainly choanocytes—become marked (Figs. 9A and 9B). Interestingly, long exposure to EdU (20 h and more) results in labeling of the cytoplasm of granular cells, while nuclei of these cells remain unlabeled (Figs. 9C and 9D). This phenomenon has been observed in all sponge samples submitted to long term exposure to EdU (e.g., Fig. 9C).
Many of the choanocytes migrating towards the wound surface after 6 h of regeneration have labeled nuclei (Figs. 9C, 9E and 9F). However, at 12 h after wounding, the labeled cells are absent at the wound surface and underlying mesohyl, although many choanocytes in the deeper chambers are labeled (Fig. 9G). This suggests that choanocytes migrating towards the wound surface to form new exopinacoderm stop their DNA synthesis, while choanocytes in the deeper chambers (even though disorganized) continue to synthesize DNA.
During the reorganization of the aquiferous system between 24 and 48 h following wounding, labeled choanocytes and mesohyl cells are randomly distributed around the wound (Fig. 9H). Thus, at this stage no local proliferation at the wound surface or in deeper layers of mesohyl can be detected. Some choanocytes with their remaining flagella can be found between the newly differentiated exopinacocytes at the wound surface (Fig. 9H).
In this work we show that in Halisarca dujardini regeneration follows a sequence of events previously reported for other demosponges (Korotkova & Nikitin, 1969a; Korotkova & Nikitin, 1969b; Thiney, 1972; Diaz, 1979; Boury-Esnault, 1976): (1) closure of injury and disintegration of the structures in the adjacent areas; (2) undifferentiated cell mass (blastema) formation; (3) epithelialization of the wound surface; (4) the reorganization of the inner damaged structures.
The starting point of eumetazoan regeneration is the healing of the wound, which often involves migration of epithelial cells toward the site of injury (Carlson, 2007; Brockes, Kumar & Velloso, 2001).
According to earlier observations in Halisarca dujardini, partial epithelialization of the wound takes place within the first 24 h of regeneration, and is completed within 3 days (Korotkova & Movchan, 1973; Korotkova, Suchodolskaya & Krasukevitch, 1983). It has been proposed that epithelization of the wound surface in this species is due to the gradual stretching of the intact exopinacoderm (Sukhodolskaya & Krasyukevich, 1984).
However, we have demonstrated here that wound epithelialization is due to incorporation of dedifferentiated mesohylar cells and choanocytes, while the peripheral exopinacoderm remains intact (Fig. 10). Until two days after injury, newly differentiated exopinacocytes, covering the wound surface, are motile, as shown by presence of multiple microvilli on their surface.
In regenerative biology, a blastema is defined as a temporary, specialized structure that forms upon amputation or injury and consists of a mass of undifferentiated cells, and which has the capability to form the missing structures by subsequent cell differentiation (Carlson, 2007). In vertebrate limb regeneration, a blastema forms by dedifferentiation of cells of the remaining tissues, which subsequently proliferate, and eventually redifferentiate into the original cell types or transdifferentiate into different cell types (Brockes, Kumar & Velloso, 2001; Vervoort, 2011).
In this work, we refer to the undifferentiated cell mass beneath a wound in H. dujardini as a blastema. This temporary structure includes dedifferentiated cells, like choanocytes, endo- and exopinacocytes, and also pluripotent cells—archaeocytes. These cells migrate from the blastema towards the wound surface to differentiate into the new epithelial structure, the exopinacoderm. In the following steps, blastemal cells also form aquiferous system elements. Similar structures have also been described during regeneration of some other demosponges, such as Halichondria panicea (Korotkova & Nikitin, 1969a), Hippospongia communis (Thiney, 1972) and Polymastia mamillaris (Boury-Esnault, 1976).
We found that choanocytes are the most actively DNA synthesizing cells in Halisarca dujardini with a few labeled cells (perhaps archaeocytes) also detected within its mesohyl. These findings are consistent with the data available for other demosponges. Study of the choanocyte chamber formation in the freshwater sponge Ephydatia fluviatilis showed that choanocytes were rapidly dividing (Tanaka & Watanabe, 1984). De Goeij and colleagues (2009) also showed that in many tropical sponges choanocytes are the most actively proliferating cell population (Alexander et al., 2014). Additionally, in Hymeniacidon perleve a high level of BrdU incorporation was observed in isolated archeocytes (Sun et al., 2007).
Importantly, we have not observed increased proliferation of the blastemal cells during Halisarca regeneration. These results are consistent with the study of Alexander et al. (2015) on the tropical species Halisarca caerulea, where they observed a decrease in the percentage of proliferative cells during early regeneration due to reduction of choanocyte growth phase while the length of the cell cycle remained the same (Alexander et al., 2015). In addition, we show in H. dujardini the undifferentiated cells migrating towards the wound surface to form exopinacoderm stop or slow down their cell cycle, while choanocytes in underlying tissues continue to proliferate.
The sources of cells participating in regeneration remain a central issue in research on regeneration (Tanaka & Reddien, 2011; King & Newmark, 2012; Rink, 2013). New cells can be generated in a variety of ways, including (1) proliferation of a resident stem cell population (Weissman, Anderson & Gage, 2001), (2) division of terminally differentiated cells (King & Newmark, 2012), (3) dedifferentiation of mature cells that acts a progenitor cell precursors or other cell types (Jopling, Boue & Izpisua Belmonte, 2011), or transdifferentiation when new cell types arise as a result of a change in state from one cell type into another (Jopling, Boue & Izpisua Belmonte, 2011; Slack & Tosh, 2001). The extent to which each mode is used varies between species and even across tissues within the same species (King & Newmark, 2012).
Traditionally archaeocytes were considered to be toti/multipotent stem cells in demosponges (Buscema, de Sutter & Van de Vyver, 1980; Sun et al., 2007; Funayama, 2013). Archaeocytes were suggested to be totipotent cells by Wilson (1907), Wilson (1910), Müller (1911) and Huxley (1921). Further investigations, mainly at the light microscopic level, supported the idea that archaeocytes are one of the more active actors of exopinacoderm regeneration (Fauré-Fremiet, 1932; Ganguly, 1960; Borojevic & Lévi, 1964; Korotkova & Nikitin, 1969a; Korotkova & Nikitin, 1969b; Thiney, 1972; Korotkova, Suchodolskaya & Krasukevitch, 1983). During the regeneration of Polymastia mamillaris incurrent papillae, it was also assumed that the new pinacocytes were derived from archaeocytes (Boury-Esnault, 1976). However, the differentiation of archaeocytes into choanocytes during regeneration has been rarely reported (Brien & Meewis, 1938; Meewis, 1938; Borojevic, 1966). Nonetheless, based on the specific expression of EfPiwiA in archaeocytes and choanocytes, it was proposed that both archaeocytes and choanocytes are components of the demosponge stem cell system (Funayama et al., 2010; Funayama, 2013). Under specific circumstances choanocytes transform into archaeocytes, indicating that even when they are fully differentiated, choanocytes maintain pluripotent stem cell-like potential (Funayama et al., 2010).
Here we show that in Halisarca dujardini there are three main sources of the new exopinacoderm during regeneration: choanocytes, archaeocytes and (rarely) endopinacocytes (Fig. 10).
One of the unexpected and striking results of our work is that the choanocytes, as well as the archaeocytes, are the main source of new tissue during ectosome regeneration. While archaeocytes directly differentiate into new cells, choanocytes and pinacocytes undergo transdifferentiation during regeneration. This is in line with cell transdifferentiation previously described in H. dujardini during somatic embryogenesis (Volkova & Zolotoreva, 1981).
There are two main types of metazoan morphogenesis: epithelial and mesenchymal, involving movement of cell sheets and individual cells, respectively (Gilbert, 2013). Transitions between epithelial and mesenchymal tissue states are referred to as epithelial-mesenchymal transition (EMT) and mesenchymal-epithelial transition (MET) (Hay, 2005; Kalluri & Weinberg, 2009). Both processes are extensively studied in Eumetazoan models, and are involved in embryonic development, asexual reproduction and regeneration (Keller, Davidson & Shook, 2003; Hay, 2005; Radisky, 2005; Kalluri & Weinberg, 2009; Ereskovsky, Renard & Borchiellini, 2013).
Here we show that EMT and MET occur during Halisarca regeneration. EMT is the principal mechanism, prominent during the first stages of regeneration (blastema formation), soon after the injury. Epithelial cells (choanocytes and endopinacocytes) from damaged and adjacent intact choanocyte chambers and aquiferous canals assume mesenchymal phenotypes and migrate into the mesohyl. After the blastema is formed, MET becomes the principal mechanism utilized during regeneration of the ectosome and the upper part of choanosome.
Our study demonstrated that regeneration in demosponges involves a variety of processes utilized during regeneration in other animals (e.g., cell migration, dedifferentiation, blastemal formation) and points to particular importance of transdifferentiation in this process. Further studies will be needed to uncover molecular mechanisms governing these processes.


text:
Kids are boon to the couples. But when it's about parenting at some age, then we should care them more! Parenting is not an easy thing.. It's an Art! We should learn parenting first to teach our kids then! Actually there are some interesting reasons behind, for the parents telling lies to their kids..
Because of the over affection and care towards the kids, the parents can't beat their kids at most of the times. Babies are always naughty! But, we should control them when they are adamant on useless things and when they do wrong activities. So, the RIGHT LIE at the RIGHT SITUATION will threaten the kids to get afraid and calm down from the adamant state. So next time whenever the kid will do the same thing, that lie will make them to remain in calmed stage till they come to their matured age!
Most of the kids love the fantasy things and stories. So if they adamant on something, especially on food feeding, the fantasy LIES on some specific things will make them to have their food happily.
Sometimes, the kids may ask adult related questions. At that time, being a good parent, they cannot answer frankly. So they need to tell LIE to make them understand in another way.
Some other times, some families' economical situation may low. At that time, when the kid asks for costly things, the parents cannot afford. At the same time, they need to calm down their kid. So, a right LIE will set a kid in happy mood without crying for it.
Some kids are really brilliant even in their growing stage. So they might ask some brilliant unknown questions to their parents to know about it. As the questions are strange & brilliant, the parents don't know the answer. So obviously they will tell some lies as answer to save their respect with kids Lol..
These lies are really harmless. In fact, "The lies telling for the Good things & Making people happy, always considered as truths only.." :)


text:
Dataquick has announced, Southern California had its worst January for home sales in nearly three years, as potential home buyers dealt with tough lending rules, and a lack of families looking to buy in the region. the new Dataquick real estate report said.
Home sales fell 9.9 percent across the six-county region from from San Diego to Norther Los Angeles in January 2013.
The Unon Tribune in San Diego announced that "prices also dipped last month, with the median falling $15,000 from December to $405,000, real estate tracker DataQuick reported Wednesday. Despite the drop, the January median is still up 15.7 percent from January 2012's median $350,000."
Dataquick Spokesman Andrew LePage said many homeowners are trying to resurface after being 'underwater' following the recession that began in 2008.
In the Inland Empire the number of homes sold was down 10 percent from January of last year.
Sales prices for Riverside and San Bernardino counties – combined – skyrocketed the past 12-months rising nearly $47,000 for the average transaction or about 23 percent.
DataQuick President John Walsh said in a statement, "Two of the bigger questions hanging over the housing market right now are, 'How much pent-up demand is left out there?' and, 'Will inventory skyrocket this year as more owners take advantage of the price run-up?'" Walsh said. "Unfortunately, we'll probably have to wait until spring for the answers. When it comes to statistical trends, January and February are atypical months that haven't proven to be predictive over the years."
Absentee buyers — investors or second-home purchasers — bought 27.5 percent of all homes sold, down from 32.4 percent in January 2013.
Dataquick Announces January Was The Worst Month for Home Sales in Nearly 3-years.


text:
Published May 31, 2017
After 40 years of dedicated service, Mike Noe is retiring from UB.
Noe has served as associate dean for community relations and clinical affairs in the School of Public Health and Health Professions (SPHHP) since 2003, a role that fits well with his clinical interests in graduate and professional training of physicians in preventive medicine, medical management, quality improvement health care, geriatric medicine and long-term medicine, and in addressing the health risks of caregivers.
"Mike served in various roles in preventive medicine, epidemiology and public health during his remarkable career here at UB," says SPHHP Dean Jean Wactawski-Wende. "His retirement will leave a void in our school and university, but we thank him for his remarkable contributions to the university and the community, and we wish him happiness in retirement."
Noe joined the UB faculty in 1975, rising to the rank of clinical professor of epidemiology and environmental health (formerly social preventive medicine), as well as clinical associate professor of medicine.
Early in his career, he taught the foundational courses in epidemiology in SPHHP's graduate program and also courses for medical students in the School of Medicine and Biomedical Sciences. Working with John Naughton, then-dean of the medical school, he was a leader in establishing the Graduate Medical-Dental Education Consortium of Buffalo, which centralized the governance and administration of more than 40 residency training programs at UB and its affiliated hospitals.
In 1991, he founded the residency program in general preventive medicine, which he continued to direct for 25 years until it moved to the Jacobs School of Medicine and Biomedical Sciences last July. During that time, he oversaw the training of physicians enrolled in this fully accredited graduate medical education program that has graduated more than 45 physicians — a number of whom remain in Western New York, including several who became UB faculty members.
From 2003-06, Noe served as interim chair of the Department of Rehabilitation Science in the new School of Public Health and Health Professions, which was created through the merger of the School of Health Related Professions with the Department of Social and Preventive Medicine in the School of Medicine and Biomedical Sciences.
In addition to his roles at UB, Noe held various senior executive positions in medical management throughout his career, including leadership roles within the Buffalo General Health System after joining the hospital staff in 1975, among them executive vice president and medical director. He also served as a vice president for medical affairs for the Kaleida Health System, the region's largest non-profit health care provider.
Noe earned his medical degree from SUNY Upstate Medical University. He completed residency training in internal medicine and preventive medicine at Tulane University Medical Center and Veterans Hospital in New Orleans, also earning an MPH degree in 1973 while at Tulane. Certified by the American Board of Internal Medicine, the American Board of Preventive Medicine and the American Board of Medical Management, he is a fellow of the American College of Preventive Medicine, the American College of Physicians and the American College of Physician Executives.
Throughout his career, Noe received numerous accolades for his service. Specific awards include membership in Delta Omega, the National Public Health Honor Society; the Dean's Award in the School of Medicine and Biomedical Sciences; the Dean's Award in the School of Public Health and Health Professions; a Distinguished Physician Award from Buffalo General Hospital; and the Program of the Year Award from the Network in Aging for the Powerful Tools for Caregivers Program, which he brought to Western New York in collaboration with the Erie County Caregiver Collation and in which he remains active. In 2007, he was named one of the Top 50 in Health Care by Buffalo Business First.
Colleagues and friends honored Noe for his many years of service at a farewell reception last week in Harriman Hall.
Office of the Dean
University at Buffalo School of Dental Medicine
325 Squire Hall
Buffalo, NY 14214-8006
(716) 829-2836


text:
Yesterday we took out the food coloring to see if we could turn this
Leprechaun Hat GREEN for St. Patrick's Day!
I wrapped some green construction paper around a cylinder vase, added a yellow band and a buckle and placed it on a circle cut from more green construction paper to look like the hat brim.
Then we added LOTS of green food coloring to the water.
Most of the white carnations did turn a light shade of green overnight.
With the extra carnations, each of the kids wanted to try a different color.
{Matthew-blue, Sarah-green, and Jillian-red}
They were pretty impressed with all the colored flowers this morning!
The kids were also excited to see the green pancakes Chris made them for breakfast.
{way to get crafty Chris!}
Happy St. Patrick's Day!


text:
Perfect Match
SWC Championship
TONIGHT - 7 p.m.
at Trumbull High
Brookfield vs. Pomperaug An impenetrable defense vs. an unstoppable offense.
In a classic match-up between two of the state's 10 remaining unbeaten football teams, Brookfield (9-0) and Pomperaug (9-0) collide tonight in the ninth annual South-West Conference championship game at Trumbull High.
Remarkably, Brookfield has yet to allow a touchdown this season, yielding only three points, while high-powered Pomperaug is averaging 36 points per game.
"It's going to be a battle," said Pomperaug coach Chuck Drury.
What makes the Bobcats' veteran defense so impressive is that so many players contribute, even after the loss of standout linebacker Mike Palo to a season-ending injury.
Linebacker Doug Sutherland and safety Kade Treibt are the top tacklers, but five Bobcats have at least 30 tackles and six others have at least 20 tackles.
"There are so many people making plays," Brookfield coach Rich Angarano said.
As one opposing coach told him, "There are no flaws."
Sutherland and Treibt are among the seven returnees from last year's unit, which carried Brookfield into the Class SS state final and is schooled by respected coordinator Mickey Consalvo.
"They get to the ball. They have good schemes. Their linebackers read the offensive blocking well and they get to the spots in a hurry," Drury said.
"They're just very stingy. They're incredible versus the run."
Which happens to be Pomperaug's trademark. Senior halfbacks Joe Melillo (129 carries, 986 yards) and Mike Kielt (130 carries, 984 yards) are among the state's most formidable duos. Melillo leads the area with 21 touchdowns; Kielt is next with 15. Both have the speed to go the distance at any time.
They operate behind an offensive line that averages 260 pounds and will be joined in the backfield by Brian Rusate, a 6-foot-1, 250-pound guard turned fullback.
"They're without a doubt the strongest football team that we have played to date," Angarano said. "They get shoe to shoe and their wings are so tight. It's going to present a challenge. We're just going to play our defense and we'll see what happens."
The Panthers plan to line up in the Delaware Double Wing using two tight ends (one of which is another lineman). They began to use the "Hammer" series, with Rusate as the lead blocker, in short yardage and goal line situations. It has since become standard operating procedure.
"The kids like it so we use it predominately now," Drury said. "You've got to coach what you coach best. We're just going to go at them."
Pomperaug doesn't do anything fancy. It simply executes.
"They're unbelievable," Angarano said. "When I watch them on film they do the same thing over and over again and they do execute it very, very well. You know what he's going to do and it's still very hard to stop.
"Our defense is extremely fast. We have team speed. But this kind of an offense is three yards and a cloud of dust. And occasionally (Melillo and Kielt), who are very fast in their own right, break it."
Brookfield's offensive philosophy is identical to the Panthers: it eschews the pass in favor of a smashmouth running game. The Bobcats' three-pronged attack features fullback John Hayduk (112 carries, 815 yards), halfback Scott Lutrus (87 carries, 707 yards) and halfback Ben Russell (92 yards, 687 yards).
Although their offensive line isn't as big as Pomperaug's, it does include 260-pound guard Ryan Pacific and 240-pound tackle Luke Badaracco on the right side. Plus, the 230-pound Russell would be a lineman, not a ball carrier, on most SWC teams.
"They're a very physical team," Drury said. "They come at you. They've got big boys, too.
"They're not on the Atkins diet over there, that's for sure. They've scored a lot of points themselves. They get the ball in the end zone. They hold the ball a lot. They've moved the ball on everybody and put up big numbers against good teams. They shorten the field on you."
Brookfield is averaging 28 points, while the Panthers are giving up 9 ppg.
Kielt is arguably among the finest linebackers in the state.
"He has a motor that never ever stops," Angarano said.
"They may have given up some points (but) they've scored a lot of points early and when you score a lot of points you get your younger guys in there early. We didn't score as many points as them and our younger guys didn't play as much," he added.
As they proved during the regular season, these teams deserve to be here. To bring home the trophy, they will have to earn it.
"We're looking forward to this championship and we think it's going to be a helluva football game," Angarano said. "It's the best against the best."
NOTES: Brookfield is appearing in the SWC title game for the first time. It won the last three Western Connecticut Conference finals (1993-95)...Pomperaug is seeking its second league title in three years, having won the program's first championship with a 17-13 victory over Immaculate in 2002.


text:
Who Runs The World?? Kelly Rowland 'Bows Down' To BeyBey's Song And Gushes About The New Album
Kelly Rowland is the president of the #BeyHive…
Kelly Rowland Talks About Beyonce And New Album
Kelly Rowland stopped by "The Big Tigger Show" to talk about her upcoming album "Talk A Good Game" which will be her fourth solo studio album.
The brown skinned beauty also had things to say about her sister's Bey's new single "Bow Down" which has been been getting trashed by everyone and their mama…
What she had to say about her album:
"I'm actually in the process of wrapping it up this weekend. This is actually the last recording session I've got for this album, but I'm so excited. I've been in the studio with Dream. I've been in the studio with Harmony. I've been in the studio with Pharrell. I've been in the studio with Kevin Cossom, who is incredible. When I tell you, he is so incredible and I'm so happy I got the opportunity to work with him."
Kelly speaks on "Bow Down":
"I've heard pieces of what she's done. She's heard things that I've recorded and I just think it scratches the surface to everything else. I think it's a great record. It's her expression. I think, as an artist, you can express yourself however you want to and I just can't wait for everybody to hear the rest of the record. It is insane."
Check out the full interview HERE
Hit the flip to peep more photos of Kelly partying it up while in ATL this past weekend…
Photo Credit: Prince Williams/ATLpics.net
More Stories From Bossip
Bossip Comment Policy
Please read our Comment Policy before commenting.


text:
An impressive high headboard combines beautifully with a low foot-end to present an elegant bedstead, rich with character. Comfort is assured with Emily's posture sprung slatted base while a choice of three fabric tones and four leg finish options provide a versatile approach to styling the wider room.
Finish: Green / Graphite / Mink
Size: Small Double | 120cm Double| 135cm King | 150cm Superking | 180cm


text: The Italian manufacturer of luxury yachts, Azimut, was established in 1969 by Paolo Vitelli and is based near Turin in Avigliana, Italy. 1970 marked the start of the company's success by expanding its operations (distribution and the design of new yachts) in partnership with Amerglass. Then later in 1985 Azimut acquired Benetti. And by so a new era started, since this acquisition allowed the company to build its own yachts. Today Azimut Yachts is considered as one of the leaders in the design and build of luxury motor yachts in the market. With 138 sites in 68 countries, Azimut Benetti group offers a wide range of motor boats going from 39 to 140 feet, and more ?


text:
Check out our Facebook page!
"Like" us to connect with other students, watch videos, see job offers and even get special discounts.
$199Apply Now!
$499Apply Now!
$1490Apply Now!
$1195Apply Now!
$175Apply Now!
$249Apply Now!
Home / TEFL Testimonials / TEFL Training Courses
Check out Teflonline.net about TEFL Training Courses and apply today to be certified to teach English abroad.
You could also be interested in:
This is how our TEFL graduates feel they have gained from their course, and how they plan to put into action what they learned:
The Problems of english Learners in ChinaAs China grows economically and expands its power in the global marketplace, more and more people are learning english not only as a means to advance themselves intellectually, but to be more and more useful in their future jobs. However, the country's english learners face many problems in their schools, including large class sizes, non-native speaking teachers, and a lack of creativity and freedom in the classroom. A readily noticeable problem in chinese classrooms is the sheer amount of students the teacher must deal with. There are over 20 million students in China's higher education system, which is a huge number of students to deal with (China Education Center). While studying in China, the author spoke to classes with an average number of 50-75 students, quite a large number of students to deal with when it comes to language learning, a high maintenance subject requiring constant attention and practice. Large classes make planning
activities problematic, as well as limiting the amount of freedom and spontaneity the class can experience in general. As the author observed, practice is largely limited to worksheets, homework is primarily reading, and lessons largely consist of teacher-centric lectures. Another problem that chinese classrooms face is the prevalence of non-native english speakers which populate the lower levels of chinese education, and sometimes can even penetrate the ranks of higher education. The problem with non-native speaking english teachers is that many areas of the curriculum can be taught incorrectly. Pronunciation is liable to be taught wrong, as well as the potential for "Chinglish" to abound. Outdated and rarely-used words and phrases often make their way into the classroom and can confuse native speakers in conversation. Because they have no way to know that what they are being taught is wrong, they take these incorrect lessons they are being taught to heart and, in turn, teach
them to the next generation. Possibly the biggest problem for english learners in China is the lack of freedom and creativity and its repercussions in the classroom. "Only 4.7 percent of chinese primary and secondary school students think they have curiosity and imagination and 14.9 percent of them hope to have imagination and creativity," writes Liang Jun of People's Daily Online ("chinese Students"). This stems from a number of factors. First, because China's population is so big, it fills the classroom to capacity and beyond. Because of that, teachers need to put their foot down in order to control the students' behavior and teach the lesson in what they see as an effective way. Second, traditionally, teachers in China command the utmost respect, and when the teacher talks, nobody else talks. The students are not allowed to have any opinions. With classes so large, how could any teacher let each student have their say? Third, the chinese education system and job
market looks fondly upon test scores and grades, not upon skills and innovation, which shows itself in China's students domination in the world's test scoring, and have manipulated their education system to be test-oriented rather than creativity-oriented ("China Beats Out"). Because of the lack of creativity, freedom, and discussion in China's classrooms, chinese students are withdrawn, rarely inclined to speak, let alone state their own opinion and engage in a discussion. These are very serious problems that China must deal with in order to truly be a world-class competitor in education. China, just like any other country, has issues with its educational system. A booming population, non-native speakers who teach incorrect english, and the lack of freedom and creativity in its classrooms all coalesce into real problems for the english teacher. The teacher must be creative and willing to try anything in order to help students overcome these barriers to truly learn to love english
and see it as a real tool for communication. Games, activity, an open, engaging attitude, and the willingness to let students experiment with the language are all key to helping chinese students overcome the shortcomings of what they were taught as younger students. Bibliography China Education. China Education Center. . "chinese Students Lack Imagination, Creativity." People's Daily Online - english. . "China Beats Out Finland For Top Marks in Education." http://www.time.com/time/world/article/0,8599,2035586,00.html
TEFL Phuket - Thailand
TEFL Cape Town - SA
TEFL New York City - USA
TEFL Rome - Italy
TEFL Paris - France
TEFL Barcelona - Spain
TEFL Prague - CZ
TEFL Zhuhai - China
TEFL Seville - Spain
TEFL Florence - Italy
TEFL Alexandria - Egypt
TEFL Bangkok - Thailand
TEFL Buenos Aires - Argentina
TEFL Bundoran - Ireland
TEFL Cebu - Philippines
TEFL Chia - Colombia
TEFL Chiang Mai - Thailand
TEFL Chiapas - Mexico
TEFL Chongqing - China
TEFL Corinth - Greece
TEFL Cusco - Peru
TEFL Ho Chi Minh City - Vietnam
TEFL Kathmandu - Nepal
TEFL Koh Kong City - Cambodia
TEFL La Antigua - Guatemala
TEFL Leipzig - Germany
TEFL Loja - Ecuador
TEFL Manuel Antonio - Costa Rica
TEFL Moscow - Russia
TEFL Singapore - Singapore
TEFL Tokyo - Japan
|All In-class Courses
|All Combined Courses
|TEFL Ann Arbor - Michigan
|TEFL Atlanta Georgia - USA
|TEFL Barcelona - Spain
|TEFL Boston Massachusetts
|TEFL Columbus Ohio - USA
|TEFL Las Vegas - Nevada
|TEFL Los Angeles - California
|TEFL Madison Wisconsin - USA
|TEFL New York City - USA
|TEFL New York City - USA
|TEFL Orange County - California
|TEFL Orlando Florida - USA
|TEFL Paris - France
|TEFL Phoenix Arizona - USA
|TEFL Phuket - Thailand
|TEFL Portland Oregon - USA
Chat with our team or leave a message!
This video shows how the theory of "Total Physical Response" (TPR) led James Asher to develop a new teaching methodology
"Like" us to connect with other students, watch videos, see job offers and even get special discounts.


text:
Saut� onion and zucchini in butter until tender. Arrange in a shallow 1-1/2 quart baking dish. Combine eggs, milk, salt, pepper, mustard and 1/2 cup cheese. Pour egg mixture over vegetables; sprinkle with remaining cheese. Bake at 375 degrees for 20 minutes or until center is set. Makes 6 servings.
Enjoy this recipe from What's for Dinner? originally published 10/2004.


text:
Apr 5 2012
Miike Snow 'Happy To You' Album Review
Tis the season for new albums fa la la la la la la la la. Yes folks, they're coming at us rapid fire at the moment. Everyone and their mother (that's a good band name) are releasing this year's new musical offerings, while we in the press weed through the lot looking for some nugget or shiny new ditty that we can cling to. As usual most of it doesn't hold up and ultimately offers very little in the way of in-depth listening and enjoyment. Not to say that I expected anything like this from Miike Snow, but you never know what that second album will hold. It's been a career killer for many an artist and band, especially following up an effort as well done as Miike Snow's self titled debut.


text:
Dear Alex,
Our boy is ten, this is a parental landmark, I feel different. I feel like a very grown up mummy having a ten-year-old!
But my day has subsided into tears. My boy, our boy, and you weren't here to celebrate it with us.
My day was engulfed with sadness, bitterness, that I held well. Monty has had a wonderful party, darlek cupcakes and his best presents EVER, he says!
HE sleeps on the floor in my room, holding his teddy in his arms. surrounded by Dr Wo lego. The lego, which he has received every year for years-being obsessed with it- that he used to sit with you at the table and construct.
He now does it alone.
No dad to be with him and do father and son things.
And I feel so sad.
So sad for him, this landmark double figures age, and you were away from us.
Friends come over with their kids and share the parenting, I see to our kids alone. No dad to play with them, give in and give them more cake, help clear up at the end of having 12 kids in the house.
These times are when I miss you most Alex. When the kids must miss you most. Because the crammed room, even with 12 kids and 6 adults, feels empty to me without you.
Tonight, I just wanted to say on all our behalves:
It's not fair.
Tonight I want the kids to have a daddy.
Tonight, I want to have a husband.
Tonight I am in bed alone, and ache, silently ache, for you.
Husband to me, father of our kids.
I am so sorry this has happened to you.
So sorry this has happened to our amazing kids.
me xxxxxxxxxxxxxx


text:
Saved Items Shopping Bag 0
Please fill out the field below, and we will send your friend a link to this page
This beautiful mother's bracelet features heart-shaped color stones of your choice to represent your loved ones. Personalize by engraving their names or a special sentiment. Most natural gemstones pictured have been subject to an enhancement process or treatment (e.g. heating, oiling, diffusion, waxing, etc.), which may not be permanent and may require special care. Generally, most treated gemstone jewelry should only be gently cleaned by rinsing in warm water and dried with a soft cloth. Please refer to the FAQ section below for more information about gemstone enhancements and special care recommendations.
Selected Style:


text:
lurking from the dark ruins...
zaterdag 7 december 2013
necromancer with her retinue
the necromancer and her bodyguard are Reaper Bones, the skeletons are from Mantic
Gepost door jack op 09:05
Labels: warbands
3 opmerkingen:
Very nice work!BeantwoordenVerwijderen
Great work. Very evocative band of ne'erdowells!BeantwoordenVerwijderen
Nice necromancer! I always considered her a dark elf with black skin and white hair, but she's very well suited for a human character too as it turns out :)BeantwoordenVerwijderen
Those Mantic undead are great aren't they :) I have them too.
Abonneren op: Reacties posten (Atom)