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This jam is now over. It ran from 2014-10-07 07:00:00 to 2014-12-01 08:00:00. View results
The CPP Quarterly Game Jam is an event hosted by the Cal Poly Pomona Game Design and Development Club. It's open throughout the duration of the Fall Quarter starting from Oct. 7 to Dec. 1. It's main purpose is to be used as a way for students to get their feet wet and start actually making games in accordance to Cal Poly Pomona's mantra: Learning by Doing.
Jam Guidelines:
Games Are Rated On:
Highest rated overall will be given a prize! So don't forget to vote, and tell your friends!
The club is hosting weekly workshops to facilitate any development. If you're new, or just want a space to develop with other people, feel free to drop by! The workshop schedules will be announced soon.
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Capt. Chris Dubois
Spotlight on You: Capt. Chris Dubois
Taking to the sky for Cope North 17
18th Wing Public Affairs | .
published: March 09, 2017
U.S. Air Force Capt. Chris Dubois, 67th Fighter Squadron F-15 Eagle pilot, prepares for flight during annual exercise Cope North Feb. 20, 2017, at Andersen Air Force Base, Guam. The equipment is maintained and inspected by Airmen from the 18th Operations Support Squadron, who ensure life-saving gear will work properly in the event of an emergency.
(U.S. Air Force photo by Senior Airman John Linzmeier/released)
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Know someone whose accomplishments, talents, job, hobby, volunteer work, awards or good deeds qualify them for 15 minutes of fame? How about someone whose claim to glory is a bit out of the ordinary – even, dare we say, oddball? If so, nominate them here.
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Eco-friendly weddings are becoming increasingly popular as people become more aware of their impact on the environment. When planning your wedding, there are a number of ways you can reduce your carbon footprint on your special day. Even making very small adjustments to your wedding plans can help the environment in a big way. From choosing your ring to deciding the venue, there are all sorts of things to consider, so here are some ideas to get you started.
Wedding Rings– People are becoming increasingly concerned about the impact of mining on the environment, so choosing a basic wedding ring is a simple way to help. The mining of metals such as Titanium, for instance, has less of an environmental impact than gold or platinum. Conflict free diamonds, recycled precious metals and wood rings are a wonderful alternative to gold and diamonds. You could always purchase a vintage ring from an antiques dealer or choose one that has been handed down through the family instead of buying a new ring.
Invitations– Send out invites that have been printed on recycled paper or card, or go completely paperless and invite your guests digitally via email. There are plenty of stationary companies using ethically sourced materials and eco-friendly printing practices.
Choosing a Wedding Dress– Buy a vintage second-hand wedding dress, then have it re-styled to suit your body shape. Alternatively hire a designer that will create a dress for you out of organic, natural fabric that has been dyed with vegetable dye. For your hair and make-up, choose products that haven't been tested on animals and are made from natural ingredients.
Picking the Venue– Choose a wedding venue close to your home and ditch the car- there are alternative methods of transport you can use to reduce your carbon footprint. Travel to the venue in style using a horse drawn carriage or a rickshaw.
Catering– Opt for organic food and local fresh produce when planning the catering for your wedding. You can offer your guests Fairtrade chocolate and make sure your wedding cake contains free- range eggs.
Decorations– When choosing your decorations, pick candles that are made from soybean wax or bees wax and use a florist that creates arrangements with locally sourced flowers. If you have the time, you could even grow your own flowers. Remember to visit second-hand stores, markets and fairs to find old pots, crockery and tableware.
Wedding Gifts– Ask your guests to donate to a particular charity as their wedding gift to you. Failing that, register your gift list with a company that offers organic products and has strong eco-friendly values.
The Honeymoon– For your honeymoon, visiting somewhere in your home country would avoid the need for flying. If you really want to travel abroad for your honeymoon, then you could choose to stay in an eco-friendly resort or do some volunteering to help other communities. There are other ways to travel besides getting on a plane too; you could travel by bus, train or boat.
Now go forth, and be a eco-friendly couple!
Related articles
- Will and Kate – No Wedding Ring?
- Top Wedding Posts from March
- 5 Ways for Couples to Save on Their Wedding
- Win Shoes for the Entire Wedding Party and More!
- Beautiful Wedding Invitations at Davids Bridal


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Have a Dirty Weekend at Home
Date: 26-Jul-14
Author: Brad Gray
There are literally hundreds of National Tree Day events happening around the country this weekend. But if you can't make it along to one of them or can't find one in your area don't feel left out. You can take part at home!
Get out into the yard or on to the balcony and plant some natives, herbs or ornamentals and then take 30 seconds to register your activity with us. By registering you'll be adding your plants to the national total. This year we are aiming for more than a million plants – so every bit helps.
And as an encouragement to register, one lucky person will win a case of Planet Ark Endorsed Orange Power Laundry Liquid to get rid of the dirt.


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Mary Kay Acne Treatment Products in Wilderville, Oregon
If you struggle with blemishes, you know difficult it is to find an acne treatment that's right for your skin. That's why Mary Kay offers acne solutions that really help. Not sure which products to pick? Talk to your Mary Kay Beauty Consultant in Wilderville about acne treatment options for you. The Mary Kay acne treatment collection targets issues like adult acne so you can eliminate blemishes and have the confidence that comes with clear skin. Check out individual acne care products that include toners and serums or use our full acne treatment regimen and get the results you really need. To learn what solution will work best with your skin, talk to you Mary Kay Beauty Consultant in Wilderville. Acne treatment has never been easier than it is with Mary Kay.


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Townsend 2800 Peeler
- High in capacity, low in COO
- Improved easy-access design
- More robust materials and long-life-parts
- Accurate peeling of very small sausages possible
- Easy sanitation
The Townsend 2800 Peeler accurately removes the inedible cellulose casing from your cooked and chilled sausages without damaging the sausage itself.
The high capacity sausage peeler can handle a wide variety of products, including very small sausages. The successful high capacity machine has been redesigned, according to market requirements.
Easy sanitation
- Electro-polished parts reduce the ability for bacteria to hide. Provides increased corrosion resistance. C-Clips (instead of bolts) on the steam tube attachment make cleaning easier and quicker.
Reduced maintenance costs
- Redesigned head increases bearing life. Bearings relocated away from the steam.
- Tool-less disassembly and assembly.
Reduced operating costs
- New in-feed sensor automatically shuts off air when no product is being fed. Interfaces with your air supply system.
- Specially designed steam chamber contains the steam keeping inside the machine and reduces harmful condensation in packaging room.
Related products
Townsend 2800 Peeler – English
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June 28, 2010
I love restaurants, cities, buildings, fashion, movies and design that deserve to be called charming. It doesn't happen very often but meeting a charming person is even better. My grandpa is the epitome of charming. He acknowledges when you enter a room and gives his full attention. He calls his wife "sunshine." When we take forever to get ready and finally make our way downstairs he says, "it was worth the wait." He tells us about his dreams where his name is always "Diamond Jim." He is constantly learning – even new technology (he recently bought a Mac iPad). I grew up admiring his love of life and the people in it and am so grateful to know such a charming man.
June 25, 2010
Great Color!
What is this color? I have no idea what you would call it…citrus, maybe? It's definitely too beautiful to be called plain old yellow. Well it's amazing whatever you call it! I don't own anything this color but I'm starting to think I really need to. If I look half as good as Michelle Williams and Lea Michele do in this color I know it needs to be in my closet. It definitely helps that the design of their dresses is flawless (thanks to Vera Wang and Zac Posen) and I absolutely love the red lips. Gorgeous!
June 23, 2010
Band Names
My husband and I have satellite radio in one of our cars and I must say I am a big fan. Not only do we have access to tons of music that's not available on normal radio but we get the track and band names for every song that's played. It's a great way to discover new artists and provides ample entertainment (we play 'Name That Tune' all the time). The thing we notice is how many random band names there are. I would love to know the story behind some of these names and how in the world they come up with them. Is it just something that they thought sounded cool? Is there a story behind the name? Maybe it's an inside joke that we aren't in on? Fascinating! I told my husband he should write a book about how bands come up with their names – that is definitely something I would read. Death Cab For Cutie? Modest Mouse? Foo Fighters? The Beatles? Got you thinking didn't I…
June 21, 2010
My Five Favorite Things About Summer - #5 LONG DAYS
How great is it that we get the sun until 9:30pm! This makes me so happy because I'm a night owl and seem to get my second wind just as everyone else is winding down. I love the idea of playing all day long, eating a late dinner and still having sunlight to play more. In the winter I feel like the day is gone before it gets started but thankfully in the summer, we get that extra sunlight to accomplish all these summer activities we have on our to-do list. What a gift!
June 18, 2010
My Five Favorite Things About Summer - #4 WATER ACTIVITES
Whether it's just lounging by the pool, on a boat at the lake or building sandcastles at the beach – I love all water activities. In fact I'd love to combine all three for the greatest summer ever! Our love for water must start in our youth, when you're young really any water will do. My summer uniform was a swimming suit just in case I came upon any sprinklers, water balloons, slip-n-slides, streams, or water guns. Then as we get older we seem to watch from the sidelines, well I say not any more. This summer let's get wet!
June 17, 2010
My Five Favorite Things About Summer - #3 BBQ's
"The Balmfy Dog"
Grilled all beef hot dog (cut in half before grilling)
Cut up Claussan pickles (other pickles don't stand a chance
against Claussen pickles, found in the refrigerated
section by the lunch meat)
Toasted bun
June 15, 2010
My Five Favorite Things About Summer - #2 SUMMER READING
Breakfast of Champions by Kurt Vonnegut
Catching Fire by Suzanne Collins (sequel to
The Hunger Games, I highly recommend)
Perks of Being a Wallflower by Stephen Chbosky
Persuasion by Jane Austen
Catch-22 by Joseph Heller
June 14, 2010
My Five Favorite Things About Summer - #1 THE HEAT
It's the first thing we think about when we think of summer – the heat. Just the thought of sunlight on my face makes me sigh. I'm a strong believer in everyone needing 20-30 minutes of Vitamin D a day and the easiest way to get it is to soak up some rays. Let's put a smile on all our faces with some heat on our skin.
June 11, 2010
Why is it that everything in life relates back to Seinfeld? The show about nothing. Way too many times I've said, "it's just like that episode of Seinfeld" and then proceed to tell what happened. This only turns out to be a good comment when the person has actually seen that particular episode otherwise the comedy falls short. But really I love this show; it's so stupid it's funny. And the fact that Jerry Seinfeld cannot act at all really adds to the overall greatness of the show. Yes, I know it was cancelled 12 years ago but thanks to reruns I can watch the madness anytime I want.
Play the Seinfeld trivia game (I got 14 out of 20, I don't know if I should be proud or if it's just sad)
June 10, 2010
Song Of The Day
I LOVE MUSIC! So occasionally I am going to share my 'song of the moment.' Discovering new music is one of my favorite things and I hope you enjoy it too. Here is my song for June 10th. Cheers!
The Morning Benders - Dreams (Fleetwood Mac cover)
June 8, 2010
The Simplest Key Lime Pie
I love easy recipes that taste like you spent hours slaving away in the kitchen. This is seriously the best simple key lime pie recipe I've found. I keep mine in the freezer for weeks and just eat a little here and a little there. Yum!
1 8oz tub cool whip (thawed slightly)
1 14oz can sweetened condensed milk
1/2 cup freshly squeezed lime juice
1 6oz preprepared graham cracker pie crust
lime zest, optional (about 1 tablespoon)
Combine the cool whip, condensed milk, lime juice and zest blend until smooth. Pour mixture into crust and smooth surface. Freeze overnight or until firm. Garnish with whipping cream if desired. Freeze up to 3 months.
Source: Robin Miller Quick Fix Meals
June 7, 2010
Hey listen up all! Memoir has had a major brand makeover. Check out our new look at:
Amazing right? Thanks Shayna, you're so talented!
June 4, 2010
A Different Time
Sometimes I wish I lived in 19th century England. It was such a fascinating time. I love the way they talked and how important a reputation was. Women worked hard developing talents to be considered 'accomplished' (even if it was just to find a good husband). They dressed up for everything and took month long vacations. What a life. Although we have come a long way since then with women's rights and class distinctions, movies and books about this time period remain my favorite to watch and read.
"No one can be really esteemed accomplished who does not greatly surpass what is usually met with. A woman must have a thorough knowledge of music, singing, drawing, dancing and the modern languages to deserve the word. And besides all this she must possess a certain something in her air and manner of walking, the tone of her voice, her address and expressions or the word will be but half deserved."
- Miss Caroline Bingley, Pride and Prejudice by Jane Austen
June 3, 2010
Wall of Frame
My mom always told me when I wanted to change up a room to switch out the throw pillows and it would make the whole room look different (great idea by the way). My idea of changing up the décor is printing and hanging new photos. Sometimes you just need a change. I love this decorating idea. The statement is in the color choice, thin white frames, large white mats and great black & white photos. It's so fun to see a boring wall become a great focal point. Make sure you choose awesome photos that inspire you and hang the frames in an artsy and creative way – it really makes all the difference.
June 2, 2010
Summer Staple
Popsicles completely remind me of my childhood. I don't know why but I haven't embraced them like I did in my younger years. Well that's about to change because it's almost summer and you feel like you can do things you normally wouldn't, case-in-point, popsicles! They are definitely a summer staple and I would like to give a shout out to the classics…
Big Stick – watch out for bright orange lips when you're done
Firecracker – very patriotic, required for the 4th of July
and the all-famous
Twin Pop popsicles – the blue ones disappeared way too fast and we were always left with a bag full of orange popsicles
June 1, 2010
A June Moment
The word 'June' doesn't quite match its month. You know what I mean? June sounds dark and rainy not sunny and green like the weather dictates. Sometimes I feel like certain words contain a mood in and of itself. Like there is a color, a feeling, even a song for each word. June does that for me. It's like those rainy days when your driving in your car – you have nowhere specific to be, the sky is gray, the dark clouds leave a shadow over everything then that perfectly June song comes on the radio and that's it. Everything aligns and you have that perfectly June moment. No one else would understand because it's different for everyone. That's what I like about it; it's just for you. And for me – I enjoy every second.
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I can't help but notice that in my lifetime, politicians have been known to be ruffians and scallywags.
This isn't exactly good for PR (public relations to the working man). I personally struggle to trust liars to even give me the right change at Tesco, let alone to run my country. The solution's simple, right? Purge our government of such people and get back to the good old days of honest politics. The crippling factor here is that that's clearly a crock of rubbish, there never was such a time. A good number of people in our House of Lords have managed to buy their way in rather than earn it, and it's only natural for politicians to abuse their power. I'm not advocating it, it's just like 'spotting' weapons of mass destruction in countries that are brimming with oil-it will happen. And I'm not here to make a political point as such, just rather with an idea for politicians to use.
Tony Blair managed to side with George Bush, one of the most hated presidents in the last hundred years, on a number of issues and made a great number of mistakes, but doesn't have anything like as bad a reputation that his replacement Gordon Brown had, who managed to make significant progress to buying us time to staving off a recession. The Europeans even wanted Blair to be president of the European Union! They eat up anything he has to say. Why? Why, a trusty doctor of course. Not the sort that's keeping Vanessa Feltz alive, or the even more annoying kind that's had half of the Osbourne family inheritance just to keep Sharon's so full of plastic that it's unmovable, and her boobs… well, you could probably break your knuckles on those. A good old spin doctor, Mr Alistair Campbell. The dark hand that loomed over Tony's shoulder was more officially known as 'Director of Communications and Strategy', while he was more publicly known as a sh*t-stirrer. The point is, it doesn't matter how slimy you are, so long as you know how you should come across to the public, you'll be in.
So here's my proposal. Much how most guys my age make a fantasy football team, I want to make my own fantasy political party, based on keeping the wool over the public's eyes while we empty the Bank of England into our pockets. It's a balance of efficiency, personal amusement, while being entirely celebrities so that the public can 'identify' with them. As leader of the cabinet, I'd endeavour to always be in costume like the time I dressed as Top Cat. The idea behind this is that it'd cheer the country up, subconsciously linking me to good times, and of course in terms of foreign affairs, nobody would try to stop Top Cat. He has a purple fedora and a cane, for God sake. As Deputy Prime Minister, I would employ Fidel Castro, on the logic that nobody would get rid of me if he's the alternative. As Secretary of State for Foreign Affairs, I would have Prince Charles, because somebody as clinically racist as him would only want to strike a deal if it really worked in my favour, and it'd amuse me to get as many foreign leaders back-handed by the Duke of Edinburgh as possible (only for my amusement, mind). Chancellor of the Exchequer would go to Deborah Meaden, well known as the one who never invests from Dragons' Den on the logic that if anyone can make the most essential cuts and still be millions of pounds in the black, it'll be her. From here, it'll look like this
Defence Secretary: Mr T.
Work and Pensions secretary: Michael Caine
Health Secretary: Brian Blessed
Education Secretary: Stephen Hawking
Transport Secretary: Jeremy Clarkson
Attorney General: Alan Rickman
Leader of the House of Commons: Danny Dyer
Minister for Cabinet Office: Reginald D. Hunter
Now, my options here may seem… interesting, but having established the paramount crucial jobs in a quasi-official way, I am giving these jobs to people who, in some way or another, are difficult to dispute. On top of all having either humorous or epic voices which are difficult to question, or intimidating ones that you wouldn't dare question, some of them have other things that could help. Stephen Hawking, as a highly reputable physicist, Mr T and Danny Dyer as people who could snap your neck like a twiglet, Blessed and Clarkson could shout you into submission, Reginald D. Hunter can easily get someone into the public eye as a filthy racist, and finally, Alan Rickman and Michael Caine could seduce and sedate you with their voices.
No I haven't particularly thought this through (surely anything that puts Mr T and Brian Blessed in power can't be), but the fact that you secretly want my all-star government is proof that the system works. It's all about how you come across, not what you do, and I personally think Britain wants to be on any side that has Danny Dyer hosting an argument between Deborah Meaden, Fidel Castro and Jeremy Clarkson.
As a closing thought, as I see an advert for Gavin and Stacey DVDs, I've decided that James Corden is just Brian Blessed on helium. Check the laughs, you'll see what I mean.


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Interested in Student Life but don't see an activity you would like to be involved in? Start a new club at DCTC! Download a club application and guide. For more information, contact: Nicole Meulemans Director of Student Life
July 29, 2011 •
Clubs & Organizations, Start a New Club! • Views: 784
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© 2019 Student Life | A Dakota County Technical College Blog | Admin Login


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11 days into June, and it's been a wonderful month so far.
Here are some news update on my life:
1. We're getting a puppy! She's an American Brittany. She'll be 13 weeks old when we pick her up Friday (this Friday! Ahhhh!). Her name is Ches (like the game)-short for Chestnut. We are ecstatic! There will probably be some full posts about her in the very near future. Expect BUNCHES of photos.
2. I'm on week four of my training program and except for yesterday's 3 mile run, I've stayed on track. Today I have a 4.2 mile walk/run interval session lined up. Waiting for Miss Brittney to get home from work and then we're off to whip those legs into shape.
3. There are 82 days until my first race! With nearly three months left, I'm pretty sure I'll be ready and raring to go.
4. I hike the Long Trail in just 12 days – yikes! I am nowhere near prepared. I'm heading into a week-long hiking trip with my cousin Spenser and my Aunt Karen. I believe they just planned out the route this past weekend. I have yet to see it, but I am pretty excited. I'm (hopefully) getting some new hiking shoes Wednesday that I plan on breaking-in all next week (I know, not really enough time, but it'll have to do). I'll try to borrow a hiking pack from one of my female hiking pals. That just leaves a tent, water filtration system, and food to worry about. You know, no big deal.
Ok, enough with the updates, onto my weekend.
This past weekend was gorgeous. A stunning, summery Vermont weekend. If only they could all be like that. I even saw some kiddos splashing around in the lakes! Brrrrr! Dustin and I spent the weekend driving around in a topless jeep. It's been a long while since the jeep's been road-legal. We definitely took advantage of the fine weather and the jeep's new legal status to cruise around the state. Friday night it was off to Southern Vermont to help my little sister prepare for her eighth grade semi-formal.
Isn't she stunning?! She's not one to dress up often, so it was great to see her all dolled up in this wicked cute dress. She's growing up so fast…
She was lucky enough to be picked up from her dance in the loud, attention-grabbing jeep. I think she was thrilled. Then she got to stroll around the grocery store at 10pm with her indecisive sister while wearing her formal garb. Again, thrilled, I'm sure.
Saturday morning began with a relaxing dip in the spa. My mom keeps that hot tub sparkling clean. I love using it at least once when I'm home. Dustin has extensive plans to build a solar powered hot tub for us. I'm sure he will attempt the build. Someday… I don't have my hopes set on enjoying that anytime soon.
Saturday afternoon we made our way to Boston to pick up some friends who flew in from Colorado. I have to shout out to Dustin's brother-in-law for kindly driving us into Boston and back to Leominster. Riding in his new SUV was a much smoother, easier, more baggage-friendly ride than the jeep would have been. We were able to enjoy a nice BBQ dinner on a new deck/patio while catching up with our friends before we headed back to Vermont.
The trip back was… a tight squeeze. 4 adults, 2 full suitcases, a box of puppy supplies, several backpacks and miscellaneous bags, all packed into this jeep:
Catherine and Ryan were able to enjoy a nice romantic cuddle session in the back seat (whether they wanted to or not).
We traveled to Randolph where we picked up a roomier vehicle and then headed from there to the NEK. We arrived way up in Lyndonville at 2:20 am, exhausted and cramped. I think we were all asleep within 5 minutes.
We were awoken on Sunday by two itty bitty kitties… Ryan's cat had kittens about 5 weeks ago. They were adorable little fuzz balls. I love kittens.
We headed out early after doughnuts and coffee, compliments of the Ward family. We traveled the winding roads south to pick up the jeep then finally headed back home. What a beautiful, beautiful day for a ride.
Today is just as gorgeous – sun, breeze, and blue skies. The summer can't get much better (and it's technically not even summer yet)!
Tomorrow I'm off to Southern Vermont once again. This time to celebrate my sister's eighth grade graduation, to kayak with my other sister, and to teach my brother to drive a manual. If I survive that car ride, I'll be back on Wednesday with two red wine updates.


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1) Lands End
Not a place I shop often, but I'm so glad I did. So many cute things! I ended up buying two canvas totes...
I can see these canvas storage bins all over my home - the linen closet, under the beds, toy storage in the playroom.... I ended up buying the smallest size to use as our 'portable' diaper storage around the house.
2) Baby Gap
Oh my word, baby cuteness. I wish A still fit into their baby sizes, because I would buy each and every one of these items. The second one down is called "Earn His Stripes" - really? I feel like Gap made these for me. Gap merchants - if you are reading - please consider making these outfits in toddler sizes. Pretty please?
3) Pottery Barn Vanessa Duvet
Clearly I'm late to the game. This is long gone from Pottery Barn...but it is available on eBay. I have always had a hard time shopping for bedding. I can never find something I love. This is one that I keep thinking about... maybe that's a sign I should buy it. I have a feeling, though, that the hubs will say its not very masculine.
Source: potterybarn.com via Jillian on Pinterest
4) Martha Stewart for Home Depot
I'll explain more in another post, but suffice it to say I'm losing steam in the hunt for a bathroom vanity. After searching the big box stores, we seem to agree on this one: it fits the budget and it fits our decor. This may be the one...
5) More Fall Outfits
I know, enough already with the fall outfits! I think this would be perfect for our family pictures this fall. Now I just need to find a great pair of boots!
6) Pinterest Quotes
These are my favorites from the week. Especially Betty's - so well said.
Source: fansofbeingamom.com via Jillian on Pinterest
I'm linking up with Jaime for WILW!
Happy Wednesday Friends!


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Windows 10 – The best Windows yet is hereWindows 10 is familiar and easy to use, with lots of similarities to Windows 7 including the Start menu. It starts up and resumes fast, has more built-in security to help keep you safe, and is designed to work with software and hardware you already have. So Why become late ? Get Windows 10 Final Build on your devices today !
If you like this article kindly Share this :)
Official Links : Download Link
- All the links are given for the latest final official released ISO files (29th July 2015 Official Release)
- Download tool is officially published by Microsoft and it let you save ISO file to your computer when finishes
- Windows 10 Home has a catch . Microsoft don't let you turn off automatic updates on Windows 10 Home. But its free, no crack required
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Campbellsville High School students were recently named to the honor roll for the fourth nine-week grading period of the 2016-2017 school year.
Students named to the list are:
All As
Freshmen – Blake Allen, Tahler Franklin, Gracyne Hash, Taliyah Hazelwood, Cole Kidwell, Aleah Knifley, Tristin Lopez, Sierra Lynch, Zoe McAninch, Katelyn Miller, Conner Riley, Grant Rinehart, Emily Rodgers, Shelby Smith and Sydney Wilson.
Sophomores – Casey Hardin, Jeremiah Jackson, Ryan Kearney, Reagan Knight, Ian McAninch, Chris Moran, Myles Murrell, Travis Nash, Elizabeth Sullivan and Brooke Tucker.
Juniors – Chanson Atkinson, Caitlin Bright, Cody Davis, Madison Dial, Alex Doss, Brookelyn Franklin, Kimberly Harden, Bryce Richardson, Lisa-Marie Richter, Matteo Rossi, Carlee Sallee, Ryan Wiedewitsch and Connor Wilson.
Seniors – Kayla Atkinson, Caylie Blair, Gabriel Clark, Iori Hashimoto, Shauna Jones, Blair Lamb, Laura Lamb, Murphy Lamb, Madison Lewis, Caroline McMahan, Deovion Owens, Latavia Shively, Breanna Spaulding, Diamond Thompson, Robert Tungate and Noah Wagers.
As and Bs
Freshmen – Abie Angel, Ben Arachi, Jewell Cox, Victoria Cox, Tyler Gribbins, Hannah Hall, Kaleigh Hunt, Gavin Johnson, Megan Owens, Siera Owens, Ketayah Taylor, Nevaeh Underwood and Patrick Walker.
Sophomores – Hailey Armstrong, Keidlan Boils, Lane Bottoms, Davon Cecil, Abbie Dicken, Josh Dooley, Brandon Greer, Randy Harris, Ruby Hatfield, Jackson Hinton, Glenn Lamer, Mariah Lowery, Treyce Mattingly, Gracie Miller, Cassi Pipes, Joe Pipes, Christa Riggs, Salena Ritchie, Hayley Stapleton, Spencer Swafford, Brandon Tucker and Tatem Wiseman.
Juniors – Makala Antle, Caneyah Bowen, Logan Cole, Jay Cox, Austin Cox, Sara Farmer, Shailyn Fields, Tristan Harvey, Wyatt Houk, Aidan McMahan, Lauren Riddle, Missy Vanorder and Darius Wright.
Seniors – Chase Atwood, Zack Bottoms, Jaleel Cowan, Michael De Oliveira, Mallory Haley, Kyrsten Hill, Aubrey Hunt, Kailey Morris, Shelby Page, Zack Settle, Tonika Spaulding, Tiffany Stagner, Brenna Wethington and Stone Williams.


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Friday, 27 March 2009
Sandnes in Wallpaper
Who thought that the glossy lifestyle bible Wallpaper would ever find something in my hometown Sandnes worth writing about? Well, they did, and it is a pretty cool thing they found too.
Photo caption (and photo) copied from Wallpaper's website:
The recent series of Norwegian Wood projects has impressed with its experimental sustainable designs in wood. This innovative open-air stage has become a focal point in the centre of the municipality of Sandnes. The glass roof covers a level, flexible space, creating an acoustic shelter within the open public square - sound is amplified during concerts and background noise is reduced. The semi-transparent roof structure is supported on bunched columns in slender sections of solid, untreated oak – reminiscent of a cluster of trees.
Find out more about this project here: Atelier Oslo
Read more about Norwegian architecture here: Architecture news: Letter from Norway
Helen & Hard is also mentioned in Wallpaper's report on the best of recent Norwegian architecture.
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There are two types of people in the world: those who have been to India and those who haven't.
Never really cared about this thing told me somewhere some time ago, but after visiting Bombay, Calcutta, Varanasi and the most of this amazing sub-continent, i do strongly quote this.
Travelling itself is an incredible experience, all countries are unique,but India, dear my friends, is the most awesome place on earth.
Welcome to Calcutta, the city of joy.
Calcutta is a city with a heart, with a soul.
I get to Kolky in the afternoon, right after a storm, and I start another socio-cultural experience, the ones you can only find in Asia, and each one is stronger the previous, more real, more true.
The smell of humanity saturates the air, from open air sewers – if they are there, to the clouds that follow the swarms of people that keep on accumulating at the railway station; an apparent chaos that hides the essence of Indianity. Everyone is busy with something and everyone has his role in creating the expressionist painting I enjoy, my senses maximized.
I don't even notice these porters, serious and sweaty carrying around humongous weights on old and crappy carts, running through the traffic jungle of ancient yellow cabs – awesome ambassador in great shape – and new and boring cars.
Rickshaw carried by slim runners in dothi and undershirts are jumping in the jams successfully bringing some people home, with bags full of vegetables for the dinner and flowers for the pooja, the constant, humble offerings Indians make to the panoply of the Gods .
Through a nice iron bridge human rivers are flowing, twisting in every direction like a loop, accompanied by a cacophony of horns and whistles, policemen yelling, hoping to earn a bit more baksheesh with some bribes. Street sellers assuring me that their dirty, dusty wares are just what I need.
I get to flowers market, right next to a temple with ghats that touch the river where worshippers take their daily baths.
The market reminds me of a mud war field, huge flower necklaces yellow and orange sleep like snakes inebriated with the noise; dark shapes move quickly filling up newspapers with colored powders, whose quantity forms a nice multicolor mountain range in a world where we sell and buy colours.
Street business and its laws count much more than street and safety codes. They just go on in their amazingly neat shirts and pants; shoe shiners happily clean up the leather wearing the feet of office men, who in turn comb their hair proudly showing their social status.
Having nice chats with these gentlemen I see the goodwill and positive attitudes, rare stuff in faraway western markets. From these guys I learn the three precepts about India: "In India all colours you can find", "Always remember India is n.1", and, most of all, "In India everything is MAXIMUM"
Liters of boiling chai are enjoyed at every crossroad. Each chai is a sweet intense experience that pushes the soul away from the earthly state high up, where the peace of senses rests. But with rupies five you can't go that far and after minutes two we are again in the human limbo fighting for spaces.
Groups of boys happily wash themselves with water from wells on the sidewalks, pumped up by kids whose play brings great joy to those above.
Countless human specimen are sleeping anywhere at any time, not bothered by the mass of life that flows thunderously at their side. A man uses the sidewalk as a pillow.
Meanwhile I notice that roads became wider and I'm on Calcutta's Wall Street, the first capital of the Raj, the Jewel of the British Empire. Buildings are Victorian-style, some of them really impressive symbolizing a special feeling among Britishers, Indians and this city.
Relative to India, at least, the center of Calcutta developed some civic sense: I pleasantly notice, amazingly, trash cans on the streets!
The shops' billboards and the shopkeepers themselves seem to be there forever, the former dusty and vintage, the latter busy in fighting dust that swarms like mosquitoes. The paint and the writings hand-painted on the walls are other splashes of color and artistic expressions, that overshadow the modern digital prints flattening the atmosphere.
Each wall is a perfect photographic set, each face is a perfect model and each moment is a perfect movie plot. No ticket is required to enjoy these life episodes that happened in Sudder Street between locals and tourists, the first in normal daily mode and the latter as charmed as me by the craziness happening before their eyes: barber shops set up next to big pot of food cooked by boys on the sidewalk, poles moving in the ether, bikes with five people on board driving through needles' eyes, clouds of mosquitoes that sometimes let us spot a sweet-store inside them, gangs of cows, dogs and goats that just don't care and live their lives roaming the overcrowded streets.
A group of Japanese is stuck in observing an itinerant piece of modern art quite popular in India: hundreds of electric wires endlessly twisted, hanging desperately from old and suffering poles.
In their Japanese perfection they will be asking themselves how come nothing is blown up yet; thinking of a regulator plan for this country I smile and I get another chai, toasting to India once more.
And in the night we play drums in the streets, we make bonfires and party; everyday in India there's a festival, and this one I'm in is one of the biggest: Holi, the festival of colours!
Shbaaaang explosions of colours in the land of colours, taste buds in tilt and maximum enjoyment for eyes, ears, nose, hands and feet. The three precepts learnt before are painted on every smiling face, today there's no rule and men and women happily play splashing water and colours on their semi naked bodies. My holy (and holey)t-shirt is eaten by the fire's flames and we all dance barefoot in brotherhood.
Sure I wasn't there in Woodstock, but these Technicolor scenes are a sober acid: we are all hippies, Jimmy Hendrix is reincarnated in India!
A deaf-mute guy, called FriendFinder, speaks everybody's language – body language, and he's the center of life in Sudder street where the exception is the rule.
Many people stay under beneficial sphere of Calcutta as long as possible because the energy here is objectively touchable. Dear my Kolky!
With my heart full I leave the city and I spend some time at the feet of Himalayas between Darjeeling and Sikkim, deep into Tibetan Buddhism, prayer flags who bring the words of Buddha in all directions through the wind and prayer wheels whose ethereal spinning helps in making good karma. It's always amazing to be up in the mountains breathing nature and energy.
A short stop in Bodhgaya, under the famous tree where Siddharta Gautama, a disillusioned Hindu noble man looking for answers about the reason of sufferings, meditating reached enlightenment becoming Buddha, whose teachings of love and compassion are the solid base on which the whole oriental culture developed afterwards.
And finally I reach the cradle of life, Varanasi, the eternal city.
The river Ganges reaches the ghats where thousands of pilgrims are making cleansing ablutions. The fire of Lord Shiva burns under Manikarnika, where from the last 2500 years every day hundreds of dead bodies are burned offering Mokhsa, liberation, to the soul whose ashes enter the holy Ganges.
This is the ultimate goal for a Hindu: escaping the cycle of life and reincarnations, Samsara, that relegate the soul to a mere human life. Once obtained Mokhsa the soul is granted eternal salvation, breaking the manifestation of life in human form, too much contaminated by material ties to achieve pure spirituality.
To be here and to live the truth of these rituals is a true lesson about consciousness: death is not the end of life, and being scared of it shows an immature approach to life.
In the west our fear of death - a strongly christian concept - terrifies us and leaves us without explanation when this tragical event strike our small lives, as if we don't know that our cycle is made of begin and end.
The earthly experience is just a small moment of the huge cycle of life.
The sadness and the rage that overcomes us when we lose somebody is also a form of energy that we should lean to control, and teaches us that we should get the personal best out of this life. About next life, I really don't know.
It's always the hardest lesson that makes us grow up.
From my rooftop I observe to the west old Benares, in the middle the holy river and on the other side the valley of death, where life fades away ritually, while ironically the sun rises from east every morning bringing new life.
Never ending Samsara.
My guru (Shiv from Woodstock!) is my key to understanding this city where life has a totally different meaning than anywhere else on earth, where you can live for a moment, in the present or 2000 years ago, or even stop the concept of time itself; where death and life are two sides of the same coin.
Tourism has in part conditioned the eternal city, well used to host pilgrims for the last millennia. But the aura of respect for Varanasi is way too big and powerful, nobody can scratch it.
Looking at the ghats in the silence of night or sunrise, walking through narrow alleys saturated with palpable deities highly worshipped by the community; attending magical poojas where the Brahmins play with fire and talk to the divine following precise and mysterious rituals, something moves inside me and I have the impression of elevating to a superior human state of comprehension.
it's very difficult to see beyond Maya, the apparent reality, but the power is all inside every single soul, Atman; the challenge is to control its energy. Everybody can fly. Magic is just a shift of thought. Too much charas?
Varanasi is the perfect summary of Indian spirituality, something that for a western eye might seem 'too much, incomprehensible and untouchable', but the city carries real signs of something that we've lost irreparably, something that the new man, as said, has to take back in order to not lose the whole path we've been through.
Soul my friend, where is our soul?
With the soul in my pockets I enter Taj Mahal in Agra, still a splendid live vision far beyond photos and posters. A perspective game makes incalculable the space between me and the Taj: now I can touch it, now it's a mile away. I have fun in watching the interactions of rich western tour groups of 50 50-years-old people with videocameras hanging from the neck and a flock of Rajastani pilgrims, with turbans and earrings grazing barefoot in the crowd, followed by wives wrapped in colorful sarees and home made jewels. Beautiful. Far away worlds skim over each other, sharing humanity.
In Rajasthan indeed the scene is repeating with turbans and sarees filling up the alleys of forts and towns as a background for the most typical postcards from India. I'm a little bit in a hurry though because in Haridwar a huge event is calling me: Kumbh Mela, the biggest religious festival on the planet.
On april 14th, lucky day for astrologers, I was among the 5 millions pilgrims that bathed in the holy waters of the river Ganges (again), running fast from the Himalayas to the Indian plateau.
It's the festival for Babas and pure people. Babas are these men who quit all the material ties with the world and live a totally spiritual life. During Kumbh Mela worshippers from any corner of the sub-continent pour in and pay homage to them acquiring credits. Religion and cash have always found a way to cooperate in human history and this festival becomes the money box for Babas, who make of spirituality their portfolio and of wisdom their business.
I'm in Delhi just before leaving India, pondering about how much this experience is one of these personal key points, understood only by who tried same feelings and seen same things, and for a moment touch upon the untouchable.
In the city spirituality remains a personal matter, and even though New Delhi has a modern and civilized character it happens that in Paharganj, the streets who welcomes every tourist and pilgrim approaching the capital, there's pure madness. The order of the government is to smash down all the abusive and crappy buildings to leave room for a new big clean avenue full of hotels worthy the new India. The way things work in India is to give nonsense orders to keep the people moving; hence the whole Paharganj is destroyed in just one night, with bulldozers and people hanging from balconies smashing walls with hammers and blowlamps.
It seems the end of an era, the end of old India that must keep up the pace with modern times. How impossible all this!
What will happen if and when the Indians will accept the pact with the devil? Our soul is going to be sellout?
If the Amazon forest is the lung of the planet, India is its soul. We are destroying our lungs and covering our soul with a new and out of place dress; right or wrong it might be, this is where we are going.
But it's ok man, no complaints.
Positive and negative energy will keep on intertwining in an infinite loop.
Everything is perfect, in its imperfection.
I'm not scared, because as Buddha said before leaving this planet: "We have all the teachings we need, now it's time to strive for our own salvation".
Of the two kinds of people describes at begin, maybe the former have some more instrument to understand and accept the present human condition, both in global and spiritual form.
The latter probably don't really care, rightly.
For who will survive the apocalypse of 2012, we will find in 2013 at Maha Kumbh Mela in Allahabad, to open a new chapter of human history.
So we have a bunch of month to enjoy this planet!
Burocratic problems don't allow me to go through Pakistan, hence I'm flying to Iran!
Goodbye, Unbelievable India.


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Basic information about the Harris County
The Harris County is located in the state of Georgia. This county occupies 474 square miles, with 10 square miles being water areas. There are 32.024 inhabitants living in the county according to the 2010 Census. As for the racial makeup of Harris County citizens, the 3 largest groups are White (79.29%), followed by Black or African American (17.19%) and Two or More races (1.49%). There are 11.823 households in the county, while 78,39% of them are family households and 21,61% represent non family households. There are 5 cities and towns in Harris County. The most populated city is West Point city and the smallest town or village is Shiloh city.


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The dramatic transformation of the High Line in Manhattan has been so successful that it has influenced other urban communities to re-examine what they're doing with their unused railroads. Just across the East River, however, Queens is aiming to transform a stretch of train track, that has been abandoned for 50 years, into what advocates are calling the QueensWay. More from the New York Times:
Now, the three-and-a-half-mile stretch of rusty train track in central Queens is being reconceived as the "QueensWay," a would-be linear park for walkers and bicyclists in an area desperate for more parkland and, with the potential for art installations, performances and adjacent restaurants, a draw for tourists interested in sampling the famously diverse borough.
Unlike the High Line, the QueensWay would welcome bicycles. While the trestles are relatively narrow, long stretches are wide enough — up to 25 feet — to accommodate walkers and bicyclists. New bike paths could connect the park to Flushing Meadows-Corona Park to the north, as well as an existing bikeway in Jamaica Bay to the south. About 250,000 residents live within a mile of the proposed park, and its backers see all kinds of ancillary benefits, from health to traffic.
This Up To Speed link is meant to share perspectives from around the world that may be of interest to our readers. We do not necessarily agree or disagree with the views and perspectives shared in those stories.


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Thomas Robert Veith, 60, of Cicero, Indiana, formerly of South Bend, died Wednesday June 14, in Riverview Hospital, Noblesville, Ind.
He was born June 17, 1945, in South Bend to Helen Marie (Ruppert) and Robert H. Veith. He graduated from South Bend Central High School in 1964. He served in the U.S. Air Force during the Viet Nam Conflict followed by service in the Air Force Reserves. He was a member of the Cicero American Legion and also served several years as a Cicero Police commissioner.
He first worked for Anacomp Company in Indianapolis, Indiana. He then purchased the Cicero Do It Center and operated it for several years. He was employed at Same Day Courier Company, Carmel, Indiana.
Survivors include a son, James Courtland Veith and his wife, Theresa, of Granger, Ind.; a sister, Judie Apt and her husband, Dave, of Rochester, Ind.; a brother, Robert H. Veith II of Indianapolis; a grandson, Trenton James Veith of Granger; two step-granddaughters, Amanda Jones and Ashley Jones of Granger, Ind.; stepchildren, Brian Dickover and his wife, Andrea, Chris Dickover and his wife, Kelley; step-grandchildren, Dylan, Jagger and Delilah Dickover, all of Noblesville, Ind.; his friend and companion; Patricia (Musselman) Overdorf of Cicero, Ind.; her parents, Wayne and Helen Musselman of Noblesville, Ind.; her siblings and their spouses, Joyce and Harry Wiggins, Gary and Pam Musselman, Jean and Ron Fleming, and David Musselman, all of Noblesville, and Diana Ritchie of Lafayette, Ind.; and several nieces, nephews, aunts and uncles.


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Pulling from traditional country and folk artists such as Patsy Cline, Loretta Lynn, and Laura Nyro, Oakland-based Eight Belles, aka singer/songwriter Jessi Philips and a rotating cast of musicians, are set to self-release their debut album Girls Underground on September 25th. Having already released the track "Buried Child" (hear it here) from the album, they've given us another taste of the record with earnest heartbreaker "Nolchi". Listen to the track below.
Plugging away since 1999, The National finally hit mainstream success with the release of their 2010 album High Violet. Of course, this entailed their first world tour, but in the new documentary Mistaken For Strangers, it's only the backdrop for the relationship between lead singer Matt Berninger and his younger brother Tom, who had no idea that these short videos he was shooting would turn into a public document of their troubled, if still loving brotherhood.
We talk with Israeli rockers Vaadat Charigim about some of their favorite records.
We talk with Yvonne Ambree and Jesse Barnes of Take Berlin about some of the records which influenced the recording of their debut EP, Lionize.
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Digital forensics specialist receives prestigious science and technology award
THE CHIEF executive of a digital forensics specialist based in Milton Keynes has been named First Women of Science and Technology 2017 at the First Women Awards in London.
The judges described Elizabeth Sheldon pictured as a "stand-out candidate" with a "fascinating career route". They added: "She works tirelessly to help keep us safe, perhaps in a way we do not always see in terms of cyber security."
In her role at Evidence Talks, she works with military, law enforcement and intelligence chiefs across the world as well as with corporate and legal clients.
Evidence Talks achieved record sales last year, including its biggest ever order from a US government agency.
Mrs Sheldon said: "'I am honoured and humbled to have won this prestigious award, the quality of the finalists was so high.
"The number of women running businesses in the security sector is still in the minority and we are working hard to encourage and nurture more women in this area to ensure this number continues to grow."
Last year, Thames Valley Police began to use Evidence Talks' SPEKTOR digital triage technology in its forensic investigation unit a year ago.
SPEKTOR collects targeted data or conducts full forensic images from removable media such as hard disk drives, USB sticks and memory cards and is already yielding impressive process improvements, said a police spokesman.
In her acceptance speech, Mrs Sheldon said: "I also want to recognise the wonderful work of our diligent police officers, military personnel and other operators who are able to keep us safer through the use of technologies such as SPEKTOR."
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Ship as Lifeboat Concept Disputed by ICV
A recent meeting at the National Transportation Safety Board in Washington, D.C. (attended by International Cruise Victims (ICV) Board representatives) discussed the new International Maritime Authority (IMO) policy of using "the ship as the lifeboat", which drew the following response from ICV.
ICV inform that the opening address to the meeting was given by the head of the IMO Safety Committee, Captain Andy Winbow.
According to Kendall Carver, Chairman of International Cruise Victims (ICV), the assumption the new policy makes is that ships don't sink, but obviously the Costa Concordia and the Korean ferry boat designed to hold close to 1000 passengers overnight could NOT be used as lifeboats.
In addition, in a major paper prepared by ICV and submitted to the NTSB, the following facts concerning safety regulations were presented:
- Lifeboats - cruise ships are permitted by the IMO to sail with only 75% of capacity.
- They allow ships to have the lifeboats several decks away from the muster points.
- Do not require special regulations for wheelchair or limited mobility passengers.
- Allows single hull cruise ships into icy waters, against the advice of all the experts.
- Permits usage of lifejackets which provide inadequate protection and need to be updated to a type that incorporates a hood, face mask and crotch strap.
Finally, the issue of flag states maintaining compliance of all safety regulations is of concern say ICV. Of particular concern to them was testimony given at the NTSB meeting by John Akhurst, a representative of the Bahamas Maritime Authority (BMA), who testified that they have only a full time staff of six technical experts in London to manage compliance of 1600 registered ships including… 226 cruise ships.
According to Carver, "It is ICV's position that these ships are not properly reviewed for safety compliance by the flag states given their limited resources and staff."


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Weight Control
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9 Tips To Get Off The Couch And
Exercise To Lose Weight
By Scott Gray
Many of us have a problem getting motivated, or started, when we want to lose weight. If only we could just wish the muffin top or love handles away. Unfortunately, the only effective way is to eat more sensibly and exercise to lose weight.
It can be easy to become distracted and put the diet and exercise regime off until tomorrow or next week. The only problem with this is that in the meantime you are more than likely getting less fit and the waistline is slowly but surely inching up.
Exercising to lose weight is great for many reasons. Exercise burns calories and fat. Exercising adequately can raise your metabolism for up to 48 hours after the workout has finished, ensuring that you are burning more calories more easily. Exercising also has the added benefit of adding muscle mass to your body, which again burns your metabolism at a higher rate.
There are many simple and effective strategies for motivation. Apply any or all of the following to make it easier to get off your couch and onto that exercise bike or into the gym:
Realistic goals should be set. You may want to lose 14 pounds in a week, but setting unrealistic goals will only end up with you becoming frustrated and throwing in the towel. If you have a lot of weight to lose, break it into portions. Look for the next significant number down. For example if you weigh 168 pounds, then try initially to get to 160. Once you have reached that magic number congratulate yourself and set a new target.
Start date. Give yourself a date to start. Make sure that by that date you have emptied the pantry and fridge of unhealthy and fattening foods. Restock the pantry with your new diet foods. Ensure your workout clothes and trainers are ready to wear. Enroll in the gym or dust off the exercise bike or home gym. Mark the date on the calendar or your personal organizer. Tell everyone that this is when you are starting so that they can help cheer you on. Count the days down in your head, prepare yourself mentally, and have your new routine worked out.
Inspiration. There is nothing like a school reunion, the start of summer, or some other big event to motivate most of us. If you have nothing big coming up, say to yourself I want to be this size by Christmas, my birthday, or next summer. When you have a firm date set for your weight loss it is much more motivating.
New routines. Incorporate your exercise routine into your life so that it quickly becomes a habit. Once it has become a habit you will feel bad when you have missed out. Be sure to do your workout at some part of the day that is convenient for you on a regular basis, otherwise you will have too many excuses to back out.
Rewards. Once you hit that new number reward yourself. There may be a pair of jeans that you can now fit into. You may want a new hair style or shirt. Some dieters take a day off their diet and eat something they have missed and then go back to their new eating habit the following day. This can be great for the strong willed, but if you are easily led astray with your food, I wouldn't recommend using food as a reward.
Personal trainers. If you cannot get off the couch, or out of bed, or out of the office at a decent hour, you may well need a personal trainer. This person will ensure that you are exercising by turning up and banging on your door if needs be. If you miss your appointment at the health club you will be hit with a hefty failure to show up fee. Personal trainers can be great to get the truly unmotivated started, although this will cost you.
Food diary. Keep a journal of what you are eating and the accompanying weight loss. If you are not losing weight you can look back over the past week and see why. Sometimes those glasses of wine or big lunches can catch up on you.
Exercise log book. An exercise log book is an excellent way to keep track of your fitness and how often you are working out. You will be able to monitor your fitness levels and become more motivated. Most people find exercise log books invaluable for making themselves more competitive with themselves. For example, if you are jogging around the block, how many times you went, or how much faster you are running.
Stationary exercise bikes can be great for the person who likes to exercise in the privacy of their home at all hours of the day or night. The great thing about an exercise bike is that it doesn't matter whether there is a heat wave or blizzard outside, you will not have to miss your workout. Stationary exercise bikes are wonderful for burning fat and calories, and improving your cardiac fitness.
People with a sore back, knees or hips are better suited to ride a recumbent exercise bike, which resembles a bucket seat with pedals out the front. The best recumbent exercise bikes come with heart monitors, magnetic resistance, and display consoles with pre-programmed workouts. Recumbent exercise bikes are safer and more comfortable than the traditional upright exercise bike.
You can get your butt off the couch and become motivated to exercise to lose weight. Any or all of the above strategies will have you leaping onto your exercise bike in no time. Now, where are the trainers?
About the Author:
Scott Gray is currently a fitness enthusiast and freelance writer who enjoys providing health and fitness tips to consumers. He maintains a fitness tips information web site where you can find information about getting in shape, losing weight and how to live a healthy life style.
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- Nano Express
- Open Access
HAI-178 antibody-conjugated fluorescent magnetic nanoparticles for targeted imaging and simultaneous therapy of gastric cancer
© Wang et al.; licensee Springer. 2014
- Received: 11 April 2014
- Accepted: 15 May 2014
- Published: 30 May 2014
The successful development of safe and highly effective nanoprobes for targeted imaging and simultaneous therapy of in vivo gastric cancer is a great challenge. Herein we reported for the first time that anti-α-subunit of ATP synthase antibody, HAI-178 monoclonal antibody-conjugated fluorescent magnetic nanoparticles, was successfully used for targeted imaging and simultaneous therapy of in vivo gastric cancer. A total of 172 specimens of gastric cancer tissues were collected, and the expression of α-subunit of ATP synthase in gastric cancer tissues was investigated by immunohistochemistry method. Fluorescent magnetic nanoparticles were prepared and conjugated with HAI-178 monoclonal antibody, and the resultant HAI-178 antibody-conjugated fluorescent magnetic nanoparticles (HAI-178-FMNPs) were co-incubated with gastric cancer MGC803 cells and gastric mucous GES-1 cells. Gastric cancer-bearing nude mice models were established, were injected with prepared HAI-178-FMNPs via tail vein, and were imaged by magnetic resonance imaging and small animal fluorescent imaging system. The results showed that the α-subunit of ATP synthase exhibited high expression in 94.7% of the gastric cancer tissues. The prepared HAI-178-FMNPs could target actively MGC803 cells, realized fluorescent imaging and magnetic resonance imaging of in vivo gastric cancer, and actively inhibited growth of gastric cancer cells. In conclusion, HAI-178 antibody-conjugated fluorescent magnetic nanoparticles have a great potential in applications such as targeted imaging and simultaneous therapy of in vivo early gastric cancer cells in the near future.
- HAI-178 antibody
- Fluorescent magnetic nanoparticles
- Fluorescent imaging
- Magnetic resonance imaging
- Gastric cancer
- Nude mice model
Gastric cancer is the second most common cancer and the third leading cause of cancer-related death in China [1–3]. It remains very difficult to cure effectively, primarily because most patients present with advanced diseases [4]. Therefore, how to recognize and track or kill early gastric cancer cells is a great challenge for early diagnosis and therapy of patients with gastric cancer.
We have tried to establish an early gastric cancer pre-warning and diagnosis system since 2005 [5, 6]. We hoped to find early gastric cancer cells in vivo by multi-mode targeting imaging and serum biomarker detection techniques [7–12]. Our previous studies showed that subcutaneous and in situ gastric cancer tissues with 5 mm in diameter could be recognized and treated by using multi-functional nanoprobes such as BRCAA1-conjugated fluorescent magnetic nanoparticles [13], her2 antibody-conjugated RNase-A-associated CdTe quantum dots [14], folic acid-conjugated upper conversion nanoparticles [15, 16], RGD-conjugated gold nanorods [17], ce6-conjugated carbon dots [18], ce6-conjugated Au nanoclusters (Au NCs) [19, 20]. However, the clinical translation of these prepared nanoprobes still exists as a great challenge because no one kind of biomarker is specific for gastric cancer. Looking for new potential biomarker of gastric cancer and development of safe and effective nanoprobes for targeted imaging and simultaneous therapy of in vivo early gastric cancer have become our concerns.
Dr. Jian Ni et al. found that the α-subunit of ATP synthase exhibited over-expression in breast cancer cell lines such as MCF-7H and MCF-7 cell line, with different metastasis potentials, and also exhibited high expression in breast cancer tissues, hepatocellular carcinoma, colon cancer, and prostate cancer [21]. ATP synthase is responsible for ATP production in oxidative phosphorylation and can work in reverse as a proton-pumping ATPase [22, 23]. ATP synthase expression is localized exclusively in the mitochondria where it generates most cellular ATP. However, ATP synthase components have recently been identified as cell-surface receptors for apparently unrelated ligands in the course of studies carried out on angiogenesis [24–26], lipoprotein metabolism [27], innate immunity [28–32], etc. by immunofluorescence, biochemistry, and proteomics analyses. Its molecular mechanism, function, and significance have not been clarified well.
Dr. Jian Ni's group prepared specific monoclonal antibody against the α-subunit of ATP synthase, named as HAI-178 antibody, and provided this to my group. Our primary studies showed that the α-subunit of ATP synthase also exhibited over-expression in gastric cancer cells and clinical gastric cancer tissues, with no or very low expression in normal gastric mucous tissues. Especially as one kind of self antibody which existed in human sera from patients with gastric cancer, this should be a potential biomarker with diagnosis value. In our previous work, we prepared fluorescent magnetic nanoparticles (FMNPs) composed of silicon-wrapped magnetic nanoparticles and CdTe quantum dots and used FMNPs-labeled MSC cells to realize the targeted imaging and hyperthermia therapy of in vivo gastric cancer [33]. We also confirmed that the prepared fluorescent magnetic nanoparticles show good biocompatibility [34].
In the present study, we fully used the advantages of FMNPs and potential gastric cancer biomarker α-subunit of ATP synthase, prepared HAI-178 monoclonal antibody-conjugated FMNPs, and investigated the feasibility of prepared nanoprobes to target in vitro and in vivo gastric cancer cells. Our results show that as-prepared nanoprobes can be used for in vivo dual-model imaging and therapy of in vivo cancer, and have great potential in applications such as dual-model imaging and simultaneous therapy of early gastric cancer in the near future.
All animal experiments (no. SYXK2007-0025) were approved by the Institutional Animal Care and Use Committee of Shanghai Jiao Tong University.
Expression of α-subunit of ATP synthase in gastric cancer tissues
HAI-178 monoclonal antibody was presented as a gift by Dr. Jian Ni. HAI-178 monoclonal antibody was used as first antibody to stain 172 specimens of gastric cancer and control gastric mucous tissues with immunohistochemistry method [35], which were collected from Xi'an Central Hospital, Xianya Hospital, Changzheng Hospital, and the First People's Hospital in Shanghai, and identified by pathological examination.
Preparation and Surface functionalization of FMNPs
FMNPs were prepared according to our previous report [36–38]. Before coupling the FMNPs with the HAI-178 antibody, we first functionalized the surface functional group of FMNPs as carboxyl group. Solutions of 95 mL ethanol and 2 mL 3-aminopropyltriethoxysilane (APS) were added to form a mixed solution and allowed to react at room temperature for 24 h. The aminosilane-modified FMNPs were separated by permanent magnet and were washed with deionized water three times then redispersed the FMNPs-NH2 in 100 mL dimethylformamide (DMF) and added with excess succinic anhydride to form a mixed solution and react at room temperature for 24 h. The carboxyl-modified FMNPs were separated by permanent magnet again and washed with deionized water three times.
Preparation and characterization of HAI-178 monoclonal antibody-conjugated FMNPs
We used a two-step process to obtain stable HAI-178-antibody-FMNPs conjugation. Solution of 1.5 mg FMNPs-COOH was dispersed in 2 mL pH 7 PBS buffer and was sonicated for 10 min. Then we mixed 1 mL of fresh 400 mM EDC and 100 mM NHSS in pH 6.0 MES buffer and rotated it at room temperature for 15 min. After this, the resulting solution was separated by magnetic field, and 1 mg/mL of HAI-178 monoclonal antibody was added to the above mixture and stirred in dark place for 2 h. To remove free HAI-178 antibody, the residual reaction mixture was separated by magnetic field and the solid remaining was washed with 1 mL of PBS buffer three times. Finally, 1 mL of 0.05% Tween-20/PBS was added to the HAI-178 antibody-FMNPs conjugation and the bioconjugation was stored at 4°C. When used, this HAI-178 antibody-FMNPs conjugation should be diluted with PBS/0.05% Tween-20. Then we used the Nano Drop device to quantify the coupling rate of HAI-178 antibody with FMNPs-COOH. Before the coupling reaction, we measured the total concentration of HAI-178 antibody. After the coupling reaction, we measured the HAI-178 antibody concentration in residual reaction mixture and calculated the coupling rate according the equation:
Coupling (%) = (1 − Concentration of HAI-178 antibody in residual reaction mixture/Total concentration of HAI-178 antibody) × 100. The as-prepared nanoprobes and pure FMNPs were characterized by transmission electron microscopy, photoluminescence (PL) spectrometry, and fluorescent microscopy.
Nanoprobes for in vitro targeting imaging of gastric cancer cells
Gastric cancer cell line MGC803 cells with over-expression of α-subunit of ATP synthase were used as target cells, and human gastric mucous GES-1 cells without expression of α-subunit of ATP synthase was used as control. The cells were cultured and collected, then were treated with 50 μg/mL HAI-178 antibody-conjugated FMNPs nanoprobes, and cultured in a humidified 5% CO2-balanced air incubator at 37°C for 4 h. Meanwhile, the MGC803 and GES-1 cells were treated with FMNPs as the control group. Afterward, the cells were rinsed with PBS three times, and then the cells were fixed with 2.5% glutaraldehyde solution for 30 min. For nuclear counterstaining, MGC803 cells were incubated with 1 mM Hoechst 33258 (Invitrogen, Life Technologies, Carlsbad, CA, USA) in PBS for 5 min. The cells were observed and imaged using fluorescence microscope (Nikon TS100-F, Nikon Instruments, Shanghai, China).
Nanoprobes for fluorescence imaging of gastric cancer-bearing nude mice
Animal experiments were performed according to Guidelines for Animal Care and Use Committee, Shanghai Jiao Tong University. Male athymic nude mice were obtained from Shanghai LAC Laboratory Animal Co. Ltd., Chinese Academy of Sciences (Shanghai, China). MGC803 cells (1 × 106) were injected subcutaneously into the right anterior flank area of the male nude mice with 4 to 5 weeks of age. The tumors were allowed to grow to a diameter of approximately 5 mm. At that point, about 40 μg HAI-178 antibody-FMNPs nanoprobes was injected into the mice (n = 3) via the tail vein. Mice were respectively monitored in a non-invasive manner at 0.5, 1, 3, 6, and 12 h to get fluorescence images. Then, tumor and major organs were collected, were placed on black papers, and subjected to IVIS Lumina imaging system (Xenogen) with emission wavelengths of 630 nm. The fluorescence images were acquired, and the total fluorescence flux for each sample was obtained. For the control experiment, mice (n = 3) were injected via tail vein with 40 μg of FMNPs and subjected to optical imaging at various time points post-injection. Identical illumination settings (e.g., lamp voltage, filter, exposure time) were used in all animal imaging experiments.
Nanoprobes for MRI and fluorescent imaging of gastric cancer-bearing nude mice
For MR imaging, gastric MGC803 cells (1 × 106) were injected subcutaneously into the right anterior flank area of male nude mice (n = 3) with 4 to 5 weeks of age. After the tumors reached approximately 5 mm in diameter, mice were injected with the HAI-178 antibody-FMNPs nanoprobes. MR imaging was performed at 6 h post-injections on animals anesthetized with 0.4% pentobarbital, using 3.0 T field intensity by GE HDX 3.0 T MR imaging instrument (GE Healthcare, Beijing, China) equipped with GE Signal Excite 3.0 T magnetic resonance imaging (MRI) software. The imaging protocol consisted of coronal and transverse T2-weighted spin echo (SE) pulse sequences. To produce T2 maps, the following imaging parameters were used: TR/TE = 1,000/10, 20, 30, 40, 40, 50, 60, 70, 80 ms; FOV = 8.0 cm; NEX = 1; slice thickness = 2.0 mm; number of excitations = 2. MR imaging was performed on the mice (n = 3) model with gastric tumor, and injected FMNPs without labeling HAI-178 antibody were used for the negative control. Then, the mice models with gastric cancer were injected with 40 μg HAI-178–FMNPs via the tail vein and imaged by small animal imaging system at 6 h post-injection [13].
Nanoprobes for integrated therapy of in vivo gastric cancer
Gastric cancer-bearing mice were randomly divided into four groups: test group 1 (10 mice) (200 μg of HAI-178-FMNPs plus external alternating magnetic field with 63 kHz and 7 kA/m for 4 min), test group 2 (10 mice) (200 μg of FMNPs plus external alternating magnetic field with 63 kHz and 7 kA/m for 4 min), test group 3 (10 mice) (100 μg of HAI-178 antibody), and blank control (5 mice) (saline). Every 2 days, the mice sizes were measured up to 14 days, then the mice were sacrificed.
Effects of HAI-178-FMNPs on important organs
The mice in test group were sacrificed after in vivo imaging. For histological evaluation, excised important organs from the heart, liver, spleen, lung, and kidney were frozen and embedded by medium at −20°C, were sectioned into 8-μm slices, were stained by hematoxylin and eosin (HE) stain method, and were observed by microscopy.
Statistical analysis
Each experiment was repeated three times in duplicate. The results were presented as mean ± SD. Statistical differences were evaluated using the t- test and considered significance at P < 0.05.
Results and discussion
Expression of α-subunit of ATP synthase in gastric cancer tissues
Clinicopathological data and ATP synthase α-subunit expression in 172 gastric cancers
α-ATP synthase expression
P value
5 (6.8%)
31 (42.4%)
37 (50.6%)
73 (100%)
Not significant
4 (4.0%)
32 (32.3%)
63 (63.6%)
99 (100%)
<2 cm
2 (11.7%)
11 (64.7%)
4 (23.5%)
17 (100%)
≥2 cm
2 (3.4%)
22 (37.9%)
31 (53.4%)
58 (100%)
Histological grade
3 (11.5%)
12 (46.1%)
11 (42.3%)
26 (100%)
7 (6.0%)
43 (37.0%)
66 (56.8%)
116 (100%)
0 (0.0%)
3 (15.0%)
17 (85.0%)
20 (100%)
TNM stage
2 (18.1%)
5 (45.4%)
4 (36.3%)
11 (100%)
2 (4.3%)
25 (54.3%)
19 (41.3%)
46 (100%)
0 (0%)
3 (23.1)
10 (76.9%)
13 (100%)
Lymph node invasion
3 (5.6%)
19 (35.8)
31 (58.4)
53 (100%)
Not significant
2 (4.5%)
13 (29.5%)
31 (70.4%)
44 (100%)
Preparation and characterization of HAI-178-FMNPs nanoprobes
In the course of preparing HAI-178 antibody-FMNPs nanoprobes, we found that the surface functionalization of FMNPs was very the key to conjugate HAI-178 antibody with FMNPs via covalent bond. We observed that carboxyl groups on the surface of FMNPs conjugated with HAI-178 antibody easier than amino groups on the surface of FMNPs. In our experiment, the average coupling rate of HAI-178 antibody with FMNPs-COOH was 80.29%.
Nanoprobes for targeting in vitro gastric cancer cells
Nanoprobes for fluorescent imaging of in vivo gastric cancer cells
Nanoprobes for MR imaging of gastric cancer-bearing nude mice
Nanoprobes for therapy of gastric cancer-bearing nude mice
Pathological analysis of important organs
Potential mechanism of targeting imaging and therapy of gastric cancer
In recent years, with the development of 'omics,' systems biology, and molecular imaging, a tendency of integration of multi-modality, multi-target imaging, and theranostic agents has emerged as a hotspot, a new concept called as 'systems molecular imaging' was suggested by Dr. Shen, which can be used to show the complexity, diversity, and in vivo biological behavior and the development and progress of disease in an organism qualitatively and quantitatively at a systems level. Ultimately, system molecular imaging should enable the physicians not only to diagnose tumors accurately but also to provide 'on the spot' treatment efficiently. It will become comprehensive research tools and technical means [39–44].
In this study, with the aim of integrating multi-mode targeted imaging and simultaneous therapy into a nanoprobe, we prepared HAI-178 antibody-conjugated FMNPs. Our previous work showed that FMNPs are very stable and have strong fluorescent signals and magnetic intensity, as well good biocompatibility. Using the strong fluorescent signals of the as-prepared nanoprobes, we successfully obtained the targeted fluorescent images of in vivo gastric cancer tissues in tumor-bearing nude mice, and using the strong magnetic signals of the as-prepared nanoprobes, we also successfully obtained MR images of in vivo gastric cancer tissues in tumor-bearing nude mice. It is confirmed that HAI-178 antibody can inhibit the growth of breast cancer cells [21]; up to date, no report is closely associated with HAI-178 antibody to inhibit growth of gastric cancer. Our results confirmed for the first time that HAI-178 antibody could be used for therapy of in vivo gastric cancer.
How to target in vivo gastric cancer cells is a key scientific problem [45]. Up to date, no specific gastric cancer biomarkers were reported. Dr. Ni et al. found that α-subunit of ATP synthase exhibited over-expression in breast cancer tissues. In our study, we confirmed that α-subunit of ATP synthase also exhibited over-expression in 94.7% of the gastric cancer specimens, which highly indicate that the α-subunit of ATP synthase may be a potential target for gastric cancer diagnosis and therapy. We also observed that the α-subunit of ATP synthase exhibited over-expression in MGC803 cells, and we used anti-α-subunit of ATP synthase antibody, that is, HAI-178 monoclonal antibody, to conjugate with florescent magnetic nanoparticles. The resultant HAI-178 antibody-conjugated FMNPs successfully realized targeted imaging and simultaneous therapy of in vivo gastric cancer, which highly suggests that HAI-178 antibody can target, recognize, and kill in vivo cancer cells, specially gastric cancer cells. Thus, the prepared nanoprobes have a great potential in applications such as targeted dual model imaging and selective therapy of early gastric cancer.
We successfully prepared novel HAI-178 antibody-conjugated FMNPs nanoprobes which can be used for targeted two modal imaging of gastric cancer and have an obviously specific targeting ability toward gastric cancer tissues at 2 h of post-injection. In addition, HAI-178 antibody-conjugated FMNPs nanoprobes also exhibited inhibition of growth of gastric cancer, as first reported in this study. The as-prepared nanoprobes also can be used for hyperthermia therapy of gastric cancer under in vitro alternating magnetic field irradiation and have great potential in applications such as simultaneous targeted imaging and targeting therapy of clinical gastric cancer in the near future.
This work is supported by the National Key Basic Research Program (973 Project) (No. 2011CB933100), National Natural Scientific Fund (Nos. 81225010, 81327002, and 31100717), 863 project of China (2012AA022703), Shanghai Science and Technology Fund (No. 13NM1401500), and Shanghai Jiao Tong University Innovation Fund for Postgraduates (No. AE340011).
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This delicious fall dessert can be served plain or with a scoop of vanilla ice cream.
Pumpkin Mousse
This delicious and light dessert would also be a good filling for a no-bake pie in a graham cracker or crushed gingersnap crust. Another festive idea would be to use it as a filling for Pumpkin Parfaits and layer with crushed gingersnaps and whipped cream.
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Wednesday, 15 June 2011
11th June 2011.
Rather belated in adding this - but Damian Pinguey visited the pond at Chippenham on Saturday where, as well as a Broad-bodied Chaser,4-spot Chaser, Azure and Blue-tailed Damsels, he saw a semi-mature Black-tailed Skimmer. The first to be reported to me this year though no doubt there are plenty about - particularly in the CWP. Surprisingly there was no Emperor present as they are usually a constant feature at the pond at this time of year! This mixed weather is possibly having some odd effects on emergences.
Posted by Steve Covey at 20:57
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