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By David P. Greisman - BETHLEHEM, Pa. — Steve Cunningham put forth the kind of performance that should have earned him long-awaited revenge in his long-anticipated rematch with Tomasz Adamek.
Should have.
Cunningham out-boxed and out-landed Adamek for the better part of 12 rounds. Only one judge saw it that way. The other two judges, however, somehow saw Adamek the winner, awarding him seven rounds on one scorecard and eight rounds on the other and giving him a controversial split decision victory.
Deborah Barnes and Dave Greer had it 115-113 and 116-112, respectively, for Adamek, with the dissenting vote from Tom Miller reading 115-113 for Cunningham.
Their first fight had ended in a split decision as well, though not with anywhere near as much controversy. Four years ago, Adamek had knocked Cunningham down three times over the course of their 12-round bout, those knockdowns proving to be the margin of victory
That bout had taken place at cruiserweight. Four years later, Adamek and Cunningham met at heavyweight, with Adamek tipping the scales at 223 pounds and Cunningham coming in at 203.5.
The question going into the bout was what difference the weigh difference would make — whether Adamek, who had been at heavyweight for more than three years, could use his additional 20 pounds to hurt Cunningham even worse than he had when both had been at cruiserweight.
But Cunningham — whose only appearance among boxing's big men earlier this year had him coming in at 207 pounds — used what some might have perceived as a disadvantage and turned it to his advantage.
Cunningham, just a few pounds above the cruiserweight limit, was faster than Adamek. He was better able to avoid what Adamek was throwing. And Adamek was less able to deal with what was coming at him.
Cunningham worked behind his jab, which seemed far stiffer than it had been in his first fight against Adamek. But while Cunningham still made the error of bringing his left hand back low, Adamek was nowhere near as able to land his right hand as a counter.
Cunningham would land 129 of 349 jabs on the night, for a 37 percent connect rate, according to statistics provided by CompuBox.
He used that strong jab, good combinations, regular movement as he circled around the ring, and the discipline not to get suckered into reckless exchanges. It also helped that Adamek seemed to be giving Cunningham plenty of respect, often waiting to explode with hard combinations until he'd heard the sound that signified that 10 seconds were left in each round.
It should not have been enough to steal the stanza.
Should not.
Adamek's mouth was open for air by the beginning of the third round. Later in that round, Cunningham sent out two jab feints before following with a strong right hand to Adamek's head.
The fourth round saw a reversal of roles — Cunningham countering a missed Adamek right hand, while Adamek missed a right hand counter over Cunningham's jab.
But it wasn't just finesse and discipline that Cunningham was doing well. He was able to earn Adamek's respect with some clean, hard punches, including a right hand in the sixth that momentarily buckled Adamek's knees.
That's not to say that Adamek didn't land. He did, including with several right hands in the eighth, and more right hands in the 10th and 11th. He was credited with landing nearly half of his power shots: 45 percent, to be exact, with 120 of 267 hitting their target.
Cunningham also had a good connect rate, going 80 of 167 with his power punches.
It was a given that Adamek's power would need to be the difference, and that was in mind as the fight entered the 12th. Adamek came out aggressively, landing several one-two combinations and leaving Cunningham reeling during the round. Cunningham stayed standing, however, and Adamek missed on subsequent home run shots.
Cunningham appeared to be the winner. And then Michael Buffer read the scorecards.
At first Buffer announced that Barnes had scored the fight a 115-115 draw, Miller 115-113 for Cunningham and Greer 116-112 for Adamek. But then Buffer announced that there had been a mistake: He had misread Barnes' score, which had Adamek ahead, thus making him the winner. But even the score for Barnes was incorrect — her tally, when added correctly from the round-by-round scores on the master was 115-113, not 115-112.
(CORRECTION: Michael Buffer says he did not misread the 115-115 scorecard he initially read for Barnes, but that it was as written — and that Greg Sirb of the Pennsylvania State Athletic Commission then came into the ring and corrected the scoring for Barnes to 115-112 for Adamek.)
Nevertheless, Barnes' score gave the former cruiserweight champion and light heavyweight titleholder his fourth straight win since his September 2011 stoppage loss to Vitali Klitschko. The 36-year-old, who originally hails from Gilowice, Poland, but now fights out of Newark, N.J., improves to 48-2 (29 KOs).
Cunningham, 36, of Philadelphia, suffered his third loss in four fights. The former cruiserweight titleholder is now 25-5 (12 KOs).
In the televised co-feature, undefeated Ukrainian heavyweight Vyacheslav "Czar" Glazkov scored a fourth-round technical knockout of Tor Hamer. The fight was stopped in Hamer's corner before the fifth round could begin.
Glazkov initially timed counters with Hamer's punches, landing at the same time, and harder. Hamer's output began to dwindle, allowing Glazkov to begin to break him down. Glazkov was able to land left hooks and right uppercuts — the latter from a dangerous distance. Hamer threw less and less, either standing directly in front of Glazkov, or moving again before resuming his inactivity. And when Hamer was ready to start fighting again, Glazkov was there, waiting for him with heavy return fire.
Glazkov would follow hard right hands with good left hooks. He followed a left hook with a left uppercut and then another right hand. And he followed a right uppercut with a left to the body. Hamer looked visibly wary of Glazkov's punches, and he looked visibly weary as the fight was increasingly getting away from him.
Glazkov, in essence, knocked the resistance out of Hamer, and then knocked the fight out of him as well.
The 28-year-old from Lugansk, Ukraine, improves to 14-0 with 10 KOs, finishing 2012 with his fourth victory of the year. Hamer, 29, of New York City, falls to 19-2 with 12 KOs, suffering his first defeat since a 2010 six-round split decision loss to Kelvin Price.
Junior welterweight Jerome Rodriguez taught Edwardo Stith a major lesson in what was Stith's pro boxing debut — don't call yourself "Untouchable" if your opponent can quickly prove that to be quite untrue.
Rodriguez pummeled Stith from the outset, beginning in the first round with a straight southpaw left hand, and continuing through to the end of the second round, when a Rodriguez barrage along the ropes left Stith reeling and forced the referee to jump in and stop the bout.
The time of the stoppage was 2:56.
Rodriguez's uppercut plagued Stith from the outset. One such landed shot had Stith trying to hold during the first round. But Rodriguez kept Stith trapped on the ropes, dug thudding punches into his body, and gave himself plenty of room to continue the onslaught.
Stith survived the first, but got forced back to the ropes again in the latter part of the second round. Rodriguez went to the body, then followed with a left uppercut, a right hand upstairs and then a left that had Stith falling back into the blue corner. The end came after a few more follow-up shots.
Rodriguez, 26, of Allentown, Pa., is now 2-0-1 with 1 KO. Stith, 29, of Philadelphia, is now 0-1.
You wouldn't necessarily expect a fight between two boxers with a combined career total of two knockouts to put on an entertaining match, but that's what Naim Nelson and Osnel Charles put forth for eight rounds. The lightweight bout ended with Nelson the winner via unanimous decision, with two judges scoring the bout 78-74 and the third seeing it 77-75.
Nelson invested early to Charles' body. Though Charles would continue to pursue Nelson over the course of the bout, that bodywork, and a couple of low blows, would slow Charles enough and allow Nelson to get away with being mobile between his combinations. At times Nelson would throw a fast flurry and then move away. Other times, Charles would punch and Nelson would then retaliate with several shots — sometimes up to a dozen, nowhere near all of them landing — to punctuate the exchange.
Nelson, 22, of Philadelphia, improves to 9-0 with 1 KO. Charles, 28, of Atlantic City, N.J., falls to 9-5-1 with 1 KO.
In the show's opener, heavyweights David Williams and William Miranda fought to a four-round draw. The scores were 39-37 for Williams, 39-37 for Miranda and 38-38. If the fight's result were based solely on physical looks, the bout should have easily been for Williams, who came in at 212.5 pounds compared to the very tubby 242 pounds on Miranda's frame. But Miranda came to fight, giving Williams difficulty from the outset.
Williams, 35, of Philadelphia, is now 6-6-2, (2 KOs). Miranda, 34, of Allentown, Pa., is now 6-5-2, with no knockouts.
In a welterweight walkout bout, Julio Dejesus and Korey Sloane fought to a four-round draw. The scorecards were 38-38 (twice) and 39-37 for Dejesus. Dejesus, 37, billed as originally from Yacobua, P.R. but now fighting out of New Jersey, is now 6-3-3 (3 KOs). Sloane, 27, of Philadelphia, is now 2-5-2 (0 KOs).
David P. Greisman is a member of the Boxing Writers Association of America. Follow David on Twitter @fightingwords2 or send questions/comments via email at [email protected]


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An Inside Look At Nationalized Health Care
By Greg L | 20 July 2009 | National Politics | 10 Comments
Congress wants to re-make our health care system into something approximating what Canadians experience. Here's a look at what Canadian health care really looks like, so you'll know what to expect if this gets rammed down our throats. In a nutshell, you'll end up paying a lot more, and your dog will get more effective medical treatment than you will. Really.
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The opinions expressed here are solely the views of the author, and not representative of the position of any organization, political party, doughnut shop, knitting guild, or waste recycling facility, but may be correctly attributed to the Vast Right-Wing Conspiracy. If anything in the above article has offended you, please click here to receive an immediate apology.
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10 Comments
startover said on 20 Jul 2009 at 2:50 pm:
Obama needs to get his head on straight ,as well as those in Congress.
The Nurse said the man couldn't get a Blood Test till he saw a Doctor.He said he tried but they said it would be 2 to 3 years. She said yes,that what it is.But said well your young,so you have time to wait.
How does she know that as many young people die. If we get this Socialize Medicine here our Young People will die before their time and we will not have a Free America.
Everyone needs to write Obama and the Congress,as this is not good for our Country.
I knew it was bad up there and I knew Socialize Medicine is bad, but this is pure
neglegent and uncalled for, for anyone to go through this mess.
Lets get our letters out today.
Thanks, for this review.
Red, White and Blue said on 20 Jul 2009 at 4:21 pm:
I have perused some of the Health Care bill- 1018 pages in all. The "government" - you know, the one that is supposed to be, By the People, Of the People and For the People, is imposing strict control over all employers - ALL - and individuals. Taxing those who elect not to get government controlled insurance - called a "non-electing employer". Your freedom of choice has been narrowed to little or none…or else. The entire bill has been hitched to the I.R.S. There are more provisions, fees, taxes, rules, regulations and compliances that will make a lawyer smile all the more – just what the doctor ordered? Yes, all 1018 pages of control, socialism and government expansion like nothing we have ever seen. I thought when we gave up our rights at airport security we were in trouble until this came along. At least at the airport, you weren't taxed because you didn't take your shoes off fast enough or lost the key to your lock.
This "bill" is beyond absurd. I know, Al Franken really turned this in as a late SNL joke!
I wish.
Then again, Mr. "Present" continues his teardown of individual choice and the total absence of real health care reform like Tort Law reform, controlling liability insurance cost to medical personnel, hospitals and emergency rooms as well as ensuring continuing medical coverage for unemployed. No one is entitled to any insurance, not just health insurance. It is not a "right".
We made it 233 years with the system that is in place. Why this sudden rush to "fix" this "broken and failed" system? The government - republicans and democrats - cannot "fix" a real broken system like social security and Medicare, how can they fix this when it is not really broke but in need of some tuning - a few steps at a time? Do we continue to trust the government with a decision so big as this? I think I have done very well deciding who, when and what health care I need. The government, with all there new taxes, fees and whatever, can NEVER improve our health care quality and system.
"AFFORDABLE" does NOT mean better!
Karla H said on 20 Jul 2009 at 5:21 pm:
I come from Canada. I left because of the creeping socialism there (creeping? hell, its communism!).
The health care system SUCKS!
1. All the good doctors left when their salaries got capped.
2. The system is overburdened by people who know how to "get the most for the least". There is no deductible.
3. There are quotas! You have lung cancer? Oh, we only do 1000 of those a year… and you are number 1001!
4. For decent care many Canucks head south of the border, where they can get a good doctor, who gets paid for his 10 years of post secondary education. I wonder what they will do now.
I suspect the lawyers (i.e. malpractice lawyers, and their buddies the malpractice insurance salesmen) are the ones making the money down here. Isn't that amazing? Lawyers… the same type of scum we have running our country.
BTW… my friends back in Canada typically pay 45% income tax. They work until June before they see income! Welcome to the machine, folks!
Anonymous said on 20 Jul 2009 at 8:28 pm:
but the nationalized healthcare is not what is being proposed…
just another fear factor from the Republicans.
Greg L said on 20 Jul 2009 at 8:58 pm:
If you bother to examine the bill, it certainly is. There's no way a taxpayer-subsidized "public option" can compete with private companies that have to make a profit, particularly under the onerous regulation this would impose. If there was going to be a level playing field, why would we need to pony up another trillion dollars in taxes to support government competition in the health care business?
What is being proposed will destroy private health care as we know it. The only thing Americans generally are concerned with are the costs for the system, many of which are a result of government mandates in the first place. If we simply got government out of the health care business altogether and reduced the regulatory burden it imposes, and reigned in lawsuit abuse, we could easily drop the cost of health insurance likely by half.
Government isn't the solution. It's the problem.
demssuck said on 20 Jul 2009 at 10:45 pm:
I hope all the a$$holes who voted for the "community leader" are happy, and are hit the hardest. I hope they regret their vote until the day they die!!!
Bridget said on 20 Jul 2009 at 11:15 pm:
Greg L brings up one of the biggest elephants in the room:
G Man said on 21 Jul 2009 at 11:02 am:
Here's someone who has read the health care bill and cites specific page references in the bill that show it IS nationalized socialized heath care. Read the bill yourself "Anonymous" if you do not believe it.
Bridget said on 21 Jul 2009 at 11:58 am:
Hey kids — can you say "Establish a comprehensive system of central government control over every significant aspect of your lives and property. We'll call it healthcare."
Cynic said on 21 Jul 2009 at 3:52 pm:
The bigger the lie the better!
Our elected officials devote all their efforts to provide benefits to the illegals and then pass the bill to the tax paying citizens.
And, why don't our elected officials and high level executive branch appointees accept the same medical plan they are foisting on us? Well, they are not stupid like we citizens are (stupid). They have a super medical plan (paid by - Guess Who) so why would they drop it for the inferior plan they have devised for us and the non-paying illegals.
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subset: commoncrawl


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Done for Now
Posted on: Sunday, December 2, 2012
I've just completed my last assignment for the semester! All that remains is a final- which is taken online/at home, and we have two days to complete, and is open-book, and is a compilation of all the quizzes we've had, so it requires no studying or study guide making. I think these cats with party hats are a good representation of how I feel. Enthusiasm masked by pretty much just wanting to sleep. :)
- Mrs. M
Labels: about me. school. social work
1 comments:
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Multimedia & Electronics
With premium audio, satellite radio, GPS and other interactive features, Subaru keeps you connected with multimedia devices for the modern adventurer.
Staying safe on the road means keeping your hands on the wheel. That's why Subaru offers Bluetooth® hands-free technology to manage phone and audio system. Bluetooth-enabled mobile phones link directly through the Subaru audio system.
Phone Integration
Models with Bluetooth give you hands-free control to make and take calls through the vehicle's audio system.
Audio Streaming
Some models also allow you to control streaming audio from internet radio or MP3 players, directed by voice-activated commands through your Bluetooth system.
Models without Bluetooth have the option of BlueConnect®. The same voice-activated controls, supporting English, North American Spanish and Canadian French languages.
GPS Navigation
Find all your destinations easily with Subaru's available navigation systems. Easy touch-screen or voice-activated controls let you set a location, find a point of interest or change course within seconds. Select models also offer traffic information, and an eco-route feature that will plot the most fuel-efficient path to your destination.
Owner? Update maps
Subaru EyeSight™
Subaru takes a look into the future with EyeSight.[1] Two cameras are mounted by the rearview mirror to monitor traffic and react to conditions even before you do. Basically, EyeSight sees the problem and initiates action to help avoid the trouble. EyeSight will also sound an alert and flash a visual warning if there's danger of a collision and will apply the brakes automatically if you don't. It can also optimize cruise control and warn drivers when they're swaying outside their lane.
Available on: Many new models.
Music, Movies & Internet
Wherever you go, you'll want to bring all the comforts of your modern home, from music playlists to online and digital entertainment.
USB & iPod Interface
Control your music device through the Subaru Media Hub or iPod® interface, located in the center console to keep it all contained. Toggle from radio to CD to iPod through the audio system or optional steering wheel controls, even wirelessly with available Bluetooth®.
Owner? Get User Guide
DVD Player
With the Subaru's available navigation systems, you can quickly go from destination guide to entertainment room, as all feature DVD playback capability. And models with the rear seat entertainment system can display the same movie in the rear seats simultaneously.
Mobile Internet
Turn your Outback into a secure Wi-Fi hotspot with the Subaru Mobile Internet accessory. It connects passengers in and around the car up to 150 feet, using patented TRU Technology and 3G network infrastructure backed with comprehensive user support.*
Owner? Get instructions
Audio System & Satellite Radio
Every road trip needs an epic soundtrack, whether you're uploading your own playlists or linking up with digital programming.
Premium Audio
Your music will come alive with the Harman Kardon premium audio system, designed with an array of speakers and up to 440 watts of power. Enjoy stunning clarity and realism as the Digital Signal Processing (DSP) enhances fidelity and harmonic richness.
HD Radio
Converts traditional analog radio signals into digital broadcasts complete with enhanced data features. Also includes additional HD programming not available through regular radio technology.
SiriusXM Radio
All new and leased Subaru vehicles with factory-equipped SiriusXM satellite radio[2] are eligible for a 4-month complimentary subscription. Hundreds of commercial-free, digital music, sports, talk and more.
Owner? Activate service


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The Hotel Hampton Inn Auburn,indiana offers the use of a TV in the rooms. Accommodation has direct dial telephone in all rooms.
Hotel Hampton Inn Auburn,indiana3 Stars
1020 SMALTZ WAY AUBURN 46706 - Auburn (In), United States View Map
- Jump to:
- Services
Our Auburn Hampton hotel has a heated indoor pool and whirlpool. We have a business center available for guests use as well as a conference room available for small groups. The Hampton Inn Auburn Indiana hotel offers high-speed internet access in all public areas as well as all guestrooms. All rooms have a mini-fridge and microwave. We love having you here at the Hampton Inn Auburn Indiana hotel
The Hampton Inn Auburn Indiana hotel is located conveniently off I-69. There are theaters and restaurants within walking distance of our hotel.
TripAdvisor vote: 4/5 Based on 275 guest reviews
Hotel facilities
- Minibus parking area
Rooms facilities
- Television
- Direct Dial Telephone


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On August 25, 2015, the Financial Crimes Enforcement Network (FinCEN) issued a notice of proposed rulemaking to prescribe standards for investment adviser anti-money laundering ("AML") programs. In addition, the proposed rulemaking requires certain investment advisers to report suspicious activity to FinCEN pursuant to the Bank Secrecy Act, and to be treated as "financial institutions" under the Bank Secrecy Act.
Investment advisers without established AML and counter terrorism financing compliance programs should prepare now. Regardless of the form the rule ultimately takes, banks and other counterparties will seek assurances that investment advisers have in place a sufficient program. For those who have implemented a program, it will be important to evaluate that program in light of the proposed rulemaking, including the following points of emphasis:
- Investment advisers would be subject to direct regulatory ramifications for failure to have a sufficient AML program;
- Investment advisers would be required to report suspicious activity through the filing of Suspicious Activity Reports ("SARs") and have a process in place to evaluate, execute, document and audit such reporting;
- Investment advisers would be brought under the definition of "financial institution" under the Bank Secrecy Act, which would result in currency transaction reporting and other reporting requirements; and
- Investment adviser AML compliance programs would likely be a point of emphasis for the SEC's Office of Compliance Inspections and Examinations ("OCIE") program, as well as counterparty diligence.


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Dog Fun 3 White
Dog Fun 3 White
Funny little pug, everybody loves them, pug drupal template is dedicated, but not limited to this little dogs that have special character and personality if you know them you have to love them. Dog drupal template is for free for any dog owner or anybody else. dog_fun_3_whiteAuthor: Alex
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hi expert!
i am new to jsp. i m using a html form to get user input. i want to collect all the information given by the user, using a javabean class with it's get and set property. and again i wat to display it to the user.
i am using eclipse ide and weblogic server.
but when i call that html form inertnet explore using
http://localhost:7001/example/userinfo.htm first i see the form and as i fill this form and press submit, controll goes to jsp page which has
<jsp:useBean id="formHandler " class="foo.FormBean" session="request">
but at this point i get an error
Parsing of JSP File '/examples/jsp/forms/process.jsp' failed:
/examples/jsp/forms/process.jsp(9): class 'foo.FormBean' could not be loaded
probably occurred due to an error in /examples/jsp/forms/process.jsp line 9:
<jsp:useBean id="formHandler" class="foo.FormBean" scope="request">
plz help me to sort out this problem.


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Course: Legal Aspects of AgriBusiness
Catalog Number: 10006140
Credits: 3.00
|Class #
|Class Dates
|Class Day(s)
|Class Time
|This class is currently not scheduled for the term you selected. Class schedules are updated daily to reflect changes or additions. Please check prior to the beginning of the term for the most up-to-date information.
COREQUISITE: 10006130 or 10006130OL Agricultural Marketing and Sales OR CONDITION: 190066 Advanced Dairy Herd Management
Description: ......provides students with pertinent information of the legal liabilities and regulations for both state and federal of an agribusiness. Course content includes legal descriptions, contractual agreements, employee and consumer rights, insurance, debt collection, as well as bankruptcy and employment protection issues as regulated by DOL, OSHA and DILHR.


text: Finished these socks a while ago they were a fun knit, I used Deborah Norville Sock Serenity Yarn in colour way Paprika. And the pattern is the Show-off Stranded Socks. The plastic duck is just there to show off, she wanted to be in the picture. I saw her in a Spotlight Store and as my favourite colour is purple I had to buy her. She sits in my craft room on top of my music player.


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Jeff Gamet, The Mac Observer's managing editor, will be speaking at the Colorado Macintosh User Group meeting on Thursday, June 10, in Boulder, Colorado. Jeff will be showing how to use Pixelmator to edit digital images, and will also share his thoughts on Apple's new iPhone 4.
The user group meeting starts at 7PM, and will be held at the NCAR Mesa Lab facility at 1850 Table Mesa Drive. Directions to the meeting along with a full line up of the evening's presentations are available at the CoMUG Web site.


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O'Farrell Cup stays in Leeton after five-run win over Yass
Yass will have to wait for their first win in the O'Farrell Cup after Leeton's successful defence on Sunday.
After making the long trek to Mark Taylor Oval for just their fourth challenge in the competition, Yass fell six runs short of what would have been a historic result.
Teenager Hugh Collins and captain Nathan Carn picked up four wickets each to halt the visitors chase.
Carn was thrilled by how his team responded after Yass loomed dangerously.
"We got there eventually but it went down to the wire," Carn said.
"It was closer than we would have wanted."
Backing up from last week's impressive batting, Daniel Muir preformed well with both bat and ball.
He topped scored with 56 before snaring two early wickets to have Yass on the back foot chasing 202 for victory.
However Yass captain David Field (57) and Nick Pollack (42) got things back on track.
They put on 86 for the third wicket before Collins made his presence felt.
The 17-year-old dismissed both and then ended a 38-run stand between Craig Irwin (15) and David Brown (33).
Yass had both wickets and overs in hand at 7-178 before Carn removed Andrew Swaffield (9) to trigger the beginning of the end with the visitors bowled out for 195.
Carn thought Collins really helped turn the tide.
"He picked up both batters that were in which stemmed their run rate and allowed us to get on top halfway through the overs," he said.
He was also a strong contributor with the bat, making 34, while Matt Dedini (36) and Jameson Booth (30) at 10 played good innings.
Carn thought his team could have been 20 runs or 30 short of good total after being bowled out for 201.
Beau Walker led the way for Yass finishing with 4-19 while Lachlan O'Mara claimed two wickets
After winning the cup twice in the past two seasons, Leeton were finally able to defend it this time around.
Carn was really pleased with the team's energy coming off a big win against Griffith.
"It was a really good team effort," he said.
"The boys all backed each other up and there was a real good energy.
"You are the hunter when you are looking for the cup but when you are the hunted it is an even bigger task to hang onto it.
"The last two years we've won it and lost it the next week so it is really good to hang onto this year."
Up next is Wagga.
Wagga has the penultimate challenge of the season and Carn is looking forward to the clash.
"Everyone loves beating Wagga," he said. "It will be a really good challenge for us."


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FA Cup Preview: Sunderland v Southampton
Sunderland head coach Gus Poyet says he will rest a number of key players for Saturday's FA Cup fifth round clash with Southampton.
Sunderland's Premier League trip to Manchester City on Wednesday was postponed due to inclement weather, allowing Poyet's star men an extra break ahead of the last-16 cup clash.
But despite the unexpected the rest for his players, Poyet said he would stick with his original plan and make several changes for the visit of Southampton.
"I was looking forward to giving a few players opportunities and that will not change," he told The Sunderland Echo.
"We have a few players who need to play and deserve to play but, for different reasons, they haven't been able to.
"I'm talking about players like (Emanuele) Giaccherini and (Ignacio) Scocco. They both need minutes, so we're going to use that game for this.
"But it will all be first-team players, we will not be taking the game lightly. I hope that we win it so we don't have to have a replay."
Sunderland, who will be without the suspended Wes Brown, have had a relatively straightforward run to the fifth round, with home victories over Carlisle United and Kidderminster Harriers to date.
And they have enjoyed even greater success in the League Cup, with Poyet's men due to take on Manchester City in the final at Wembley on March 2.
Sunderland remain in trouble in the league, however, sitting 18th, albeit with a game in hand over most of their rivals.
Southampton, on the other hand, are enjoying a fine season, and are eighth in the Premier League table.
Mauricio Pochettino's men come into the game on the back of an eight-match unbeaten run in all competitions, including wins over Championship outfits Burnley and Yeovil Town in the third and fourth rounds of the FA Cup.
The two sides met last month in the league, with Sunderland coming from two goals down to claim a 2-2 draw.
And Southampton assistant manager Jesus Perez says his side have learned their lesson as they look to reach the last eight of the competition for the first time in nine years.
"We know Sunderland very well," he said.
"This will be the fourth time we've met them (this season) but, when you are playing a lot of competitions, that's always a possibility.
"We are ready to face them. We have learned from every game against them. We review and analyse every game to determine how we have to improve in case we have to repeat situations."


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Danielle Shanahan, Australia
The camper was good - well suited to our needs. I was pleased for the extra space over the 2-berth as you had suggested Gigi.It ran well, no issues. Took some time for us to get away from the depot as there was a battery problem (over an hour), but at least they sent us away with a functioning vehicle. Kettle was broken, but they happily reimbursed us for the price we paid for a replacement.
I was really glad you were able to accept credit card payment in the end. It would have been very challenging for me to arrange payment by bank transfer from an overseas account. If credit cards are not ideal for you guys, I wonder whether you would consider other options in the future such as paypal?
Thanks, for a second time around, for all your help and assistance.
Posted by Gigi - 27 August, 2014 at 10:09 am


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4805 777 Richards Street, Vancouver Listing #: R2291621
A unique opportunity to own this stunning well priced NORTH WEST corner residence perched high above the 48th floor at Telus Garden by WESTBANK. Inside a bright 3 bdrm, 2 bath + Flex space offering a flawless floor plan. Over 1400 Sqff of indoor/outdoor living inclusive of a 265 SQFT WRAP AROUND SUNDECK offering SPECTACULAR CITY-SKYLINE, OCEAN, MTNS, ENGLISH BAY SUNSET views. Some of the features included an entertainment size living area, gourmet kitchen with Miele appliances, fully separated bedrooms, curtain wall system to maximize views, hardwood flooring main areas and air conditioning. Amazing amenities including 24/7 Concierge, whirlpool, exercise center, sauna/steam room, lounge areas, dog park, garden & more. Includes 2 SXS PKG.
Taxes (2017): $3,855.33
- Bike Room
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The History Center
The History Center is a non-profit organization whose purpose is to preserve and encourage access to its unique collections, while designing learning experiences and services that create opportunities for people to learn about themselves and their place in the world.
With this objective in mind, we organize exhibitions, educational programs, and partner with a diverse range of non-profit organizations throughout Tompkins County to bring original, research-based educational services to the public.
The History Center is located in the Gateway Center on 401 East State Street, Ithaca, NY, five minutes walking from the Commons, and is open to the public on Tuesdays, Thursdays and Saturdays from 11am to 5pm. (Pictured above).
The Eight Square Schoolhouse
The History Center also owns, and recently restored, the Eight Square Schoolhouse, an historic 1827 schoolhouse home to The History Center's living history program. The Eight Square Schoolhouse is listed on the National Register of Historic Places (pictured right).
Eight Square schoolhouse is located on Upper Hanshaw Road in Dryden, New York. Upper Hanshaw Road is approximately 1/4 mile East of the Tompkins County Regional Airport.


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Sale Date Ended
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Cycling is the most effective way to stay fit, Whether you're cycling to work, to school, to the shops or just for fun, the humble bicycle is an easy way to get more active and in turn save the environment (No Smoke!) . Heart disease, type 2 diabetes and stroke are easily avoided with regular cycling. It also boosts your mood and keeps your weight in-check.


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Manchester City hold the advantage in the tie, after winning 5-3 in the first leg at the Etihad stadium.
Guardiola believes the only way to beat Monaco, who has scored 123 goals in all competitions is to score goals.
"Even when I was at Barcelona and Bayern Munich I could not assure that we would win that Champions League," he told a pre-match news conference.
"In that way [attacking] is the only way it can happen. It is the only way I know
"Monaco play to attack, our team play to attack. I'm pretty sure it is going to be a fantastic game.
"When a team scores 123 goals it is because they are able to score in many, many different ways.
"The best way [to beat them] is scoring goals – take the ball and attack as much as possible is the only way to play this kind of team.
"If in 90 minutes you are thinking about 5-3, you kill yourself."


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The number of rape cases across the country has increased with Madhya Pradesh reporting highest number of such crimes, according to an official data.
In most of these cases, the perpetrator of the crime was an acquaintance with the victim, according to data provided by the National Crime Records Bureau.
- Boy drugged & sodomised by cab driver goes missing
- UP: Dalit girls raped but police call cases political canards
- Russia seeks action in Goa rape cases
- 2009: Madhya Pradesh tops rape cases list
A total of 21,467 rape cases were reported in 2008, registering an increase of 3.5 per cent over the previous year. Provisional data for 2009 shows that 21,397 rape cases were reported during the year.
Madhya Pradesh reported as many as 2,937 cases, accounting for 13.7 per cent and highest of total rape cases, followed by West Bengal and Uttar Pradesh at 2,263 and 1,871 cases respectively.
These states were followed by Maharashtra (1,558), Assam (1,438), Rajasthan (1,355) and Bihar (1,302). The lowest number of cases were reported from Nagaland (19).
Women in the age-group of 18-30 years appeared most vulnerable to the crime.
In 2008, 57.2 per cent (12,299) of the victims were from this age group, only 0.5 per cent less than in 2007 (11,984). In as many as 91 per cent (19,542) of these cases, the offenders were known to the victims. Neighbours were accused in 33.1 per cent of rape cases.
Worried over these figures, the Consultative Committee of the Parliament attached to the Ministry of Women and Child Development at its recent meeting decided to focus on the principle of restorative justice to help the victims of the heinous crime survive.
"Rape is one of the most violent forms of crimes against women, which not only impacts her bodily integrity but in the long-run, impairs her capacity to develop meaningful personal and social relationships, and affects her life and livelihood," Minister of State for Women and Child Development Krishna Tirath said in the meeting.
She said the Supreme Court had directed the National Commission for Women (NCW) to evolve a "scheme so as to wipe out the tears of unfortunate victims of rape".
The Apex Court observed that with regard to Directive Principles of the Constitution, it was necessary to set up a Criminal Injuries Compensation Board, as victims are too traumatised to continue in employment in some cases.
Following this order, the NCW held consultations with various stakeholders such as women's organisations, NGOs, members of the judiciary and police and drafted a scheme.
This comprehensive scheme titled "Financial Assistance and Support Services to Victims of Rape: A Scheme for Restorative Justice" was formulated and discussed in a meeting held last week.
Some of the salient features of this proposed scheme are that it covers rape victims in all age groups. Legal heirs are also entitled to financial assistance where death of the affected woman has resulted as a consequence of the crime.
The victim will also be entitled to interim financial assistance of Rs 20,000 to take care of her immediate needs.
Support services such as shelter, counselling, medical aid, legal assistance, education and vocational training shall also be provided.
Assistance of upto Rs 50,000 can be provided for these support services and final financial assistance of Rs 1.30 lakh to address her long-term needs.
In cases, involving minors or mentally-challenged/ differently-abled women, women who got infected with STDs or pregnant due to the crime, the quantum of assistance may be enhanced up to Rs three lakh.
In case of victim's death, her legal heirs, including minors, shall be entitled to Rs one lakh as assistance if she was a non-earning member or Rs two lakh in case she was an earning member of the family.
The scheme envisages the setting up of criminal injuries relief and rehabilitation boards at the district, state and national levels.
With inputs from PTI


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The latest Nintendo Switch stock tracking information for online and in store availability for Wednesday, May 24th.
On May 24 GameStop offers one Nintendo Switch bundle online priced at $398.99. The Nintendo Switch with Neon Joy-Con bundled with Mario Kart 8 Deluxe physical game and accessories ships within 24 hous from gamestop.com.
ToysRUs is selling a Nintendo Switch bundle including three games and accessories for $499.99 online. The GameStop and the ToysRUs bundle are the only way to buy a Nintendo Switch online today so far. To find Nintendo Switch in stock online, shoppers can use our new The Tracker app, available as free download for iOS and Android.
Nintendo believes the Nintendo Switch will be as big as the extremely successful Nintendo Wii. Looking at the current shortage, the Japanese video game maker has a long road ahead of them to ship 101 million Nintendo Switch consoles. The positive reception and praise of the Nintendo Switch fuel the demand for the mobile and home console hybrid. Nintendo is also busy marketing the Nintendo Switch with hands-on events and at the E3 2017.
Find below the latest Nintendo Switch online availability updates and stock tracking updates for stores.
Nintendo Switch Stock at Online Stores
GameStop is offering one Nintendo Switch bundle for under $400. Yesterday the retailer still had two bundles available for under $400. The Zelda bundle sold out GameStop also runs a big video game accessories sale, including discounts of 20% on select Switch accessories.
ToysRUs sells a $499.99 Nintendo Switch bundle online on toysrus.com.
Amazon restocked the Nintendo Switch on Wednesday last week. Only Prime members got access to the Nintendo Switch stock. When the next Switch sale on Amazon takes place is not known. Use our new Tracker app to get notified when the Switch is in stock at Amazon.
Last Friday at 5:01pm ET, Walmart is sold again the Nintendo Switch online at walmart.com. This was the 7th time Walmart sold the Nintendo Switch on Fridays. The Switch will be available as a custom bundle. Through the Walmart app the $299 Switch consoles have been available too. To get an notification when Walmart is again selling the Nintendo Switch online next week try our new Tracker app.
The Switch is extremely hard to score on Friday at walmart.com. A lot of successful orders got cancelled afterwards as Walmart sold more than they had available. It is a head-scratcher why Walmart's shopping platform is not able to keep sales and stock in sync.
At this point no online retailer is selling the $299.99 Nintendo Switch consoles. The Nintendo Switch will continue to be hard to find for the foreseeable future. Nintendo plans to ship 10 million consoles world-wide in the time from April 2017 to April 2018. From the launch on Mach 3 to April 27, the Mario company sold 2.74 million Switch consoles.
Online stores restocked the $299.99 Nintendo Switch with Gray Joy-Con and the $299.99 Nintendo Switch with Neon Joy-Con in time for the launch of Mario Kart 8 Deluxe on April 28. Since then the Switch console was out of stock online, besides the TRU bundle offer, according to data from The Tracker.
The Nintendo Switch reseller markups keep steady and range today between $93 to $110 for the Nintendo Switch with Gray Joy-Con and $100 to $130 for the Nintendo Switch with Neon Joy-Con on amazon.com. The Nintendo Switch with Neon Joy-Con is more rare than the console with Gray controllers, resulting in considerable higher market value. Overall the Nintendo Switch market prices have risen slightly.
To find Nintendo Switch in stock online, shoppers can use our new The Tracker app, available as free download for iOS and Android.
There are also several new accessories coming on the market from Nintendo in the next weeks. See the full list of new Nintendo Switch accessories.
When is Nintendo Switch in stock online again?
Nintendo is ramping up the production for the Nintendo Switch, but has not disclosed what the current production capacity per month is. The demand for the Nintendo Switch will spike during the Holiday Shopping season 2017. Nintendo will set aside a large part of the Switch production during the next months for November and December. This stock piling will keep the Nintendo Switch in short supply during the next 5 months.
As Nintendo coordinated new Switch console shipment with the launch of Mario Kart 8, we expect the company to do the same for the next major Switch game launches. This means the next big wave of new Switch consoles comes in mid-June for the release of ARMS.
The Nintendo Switch will likely be offered in special bundles in time for the Holidays. A Mario Kart 8 Deluxe Switch bundle has already surfaced in Russia. Because of the incredible demand for the Switch console, the monetary savings on these bundles will be limited. Usually bundles offer a way for consumers to get a free game. Nintendo can afford to just offer some savings, unless the demand is slowing down. Read the latest Nintendo Switch news.
How to find a Nintendo Switch in Stock Online
We developed the The Tracker app to make finding stock of rare products online as easy and stress free as possible. Download the free app for Apple iPhone or Android phones. From the list products you select to be notified for the Switch with Grey Joy-Con and/or Neon Joy-Con.
That is it. It is set and forget. At the moment we detect the Nintendo Switch console in stock at major online retailers, you receive a notification on your smartphone. Now you are just a tap away from buying a Nintendo Switch. You can also see the current availability of the Nintendo Switch consoles in the web version of The Tracker: Nintendo Switch with Gray Joy-Con, Nintendo Switch with Neon Joy-Con.
Nintendo Switch in Stock at Stores
Best Buy stores had the Nintendo Switch in stock this morning. We prepared our readers for this Switch event before it was official. The Nintendo Switch is also available in 710 Walmart stores, 487 Target stores, 29 GameStop locations according to the Nintendo Switch inventory tracker iStockNow (9:00am ET).
The Nintendo flagship store in New York said on April 28 in a tweet to have the Nintendo Switch in stock each morning. People in New York or visitors can give this purchase option a try. Social media posts from people who visited the flagship store confirm the availability of the Switch at the Nintendo store in New York. Some even report that they saw the Switch still on the shelves late afternoon.
This week two new Switch games are released. The big upcoming Switch games are Arms on June 16 and Splatoon 2 on July 21. Amazon offers pre-order discounts on both of the titles for Prime members to the tune of 20%. Read the latest Nintendo Switch news.


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Characterization of individual polynucleotide molecules using a membrane channel
We show that an electric field can drive single-stranded RNA and DNA molecules through a 2.6-nm diameter ion channel in a lipid bilayer membrane. Because the channel diameter can accommodate only a single strand of RNA or DNA, each polymer traverses the membrane as an extended chain that partially blocks the channel. The passage of each molecule is detected as a transient decrease of ionic current whose duration is proportional to polymer length. Channel blockades can therefore be used to measure polynucleotide length. With further improvements, the method could in principle provide direct, high-speed detection of the sequence of bases in single molecules of DNA or RNA.
Measurement of ionic current passing through single ion channels in biological membranes or planar lipid bilayers is routine in neurobiology and biophysics (1, 2). Although most such channels undergo voltage- or ligand-dependent gating (3, 4), several relatively large ion channels, including Staphylococcus aureus α-hemolysin (5, 6) and the mitochondrial voltage-dependent anion channel (7, 8), can remain open for extended periods, thereby allowing continuous ionic current to flow across a lipid bilayer (9, 10). We reasoned that a transmembrane voltage applied across a continuously open channel of appropriate size should draw polyanionic DNA or RNA molecules through the channel as extended linear chains whose presence would detectably reduce or block normal ionic flow. Such blockages should make it possible to use single channel recordings to characterize the length and, possibly, other characteristics of the polymer.
Random coil polymers have previously been used to investigate channel pore dimensions (8) and single channel current recordings show that small poly(ethylene glycol) polymers partition into channels formed by alamethicin (11) or S. aureus α-hemolysin (12, 13). Recent investigations show that proteins can also traverse a lipid bilayer by moving through protein translocating channels, presumably as unfolded, extended chains (14, 15, 16, 17, 18). While translocating a protein, such channels are electrically silent; they regain their ion permeability only after the translocating polypeptide has been cleared from the channel (18). The ionic conductance of large nuclear pore complexes is similarly reduced during translocation of transcription factors, although in this case the macromolecules are assumed to move through the nuclear pore in a folded state (19, 20).
To determine whether nucleic acid polymers can be detected by single channel measurements, S. aureus α-hemolysin was used to form a single channel across a lipid bilayer separating two buffer-filled compartments (21). The α-hemolysin monomers spontaneously insert into lipid bilayers (9) and assemble to form heptameric transmembrane channels (6) that are 2.6 nm in diameter (L. Song, M. R. Hobaugh, C. Shustak, S. Cheley, H. Bayley, and J. E. Gouaux, personal communication). Channels with this dimension should be sufficiently large to accommodate the diameter of an extended, single-stranded polynucleotide.
Conductance Measurements.
A solvent-free bilayer membrane of diphytanoyl-PC (Avanti Polar Lipids) was formed across a ≈0.1-mm-diameter orifice in a 25-μm thick Teflon partition separating two buffer filled compartments (ref. 23, patterned after ref. 21) each containing 1.5 ml of 1 M KCl, 5 mM Hepes (pH 7.5). α-Hemolysin channels were reconstituted into the membrane by adding less than 1 μg of α-hemolysin to the compartment we designated as cis. A single channel usually formed within 5 min. To prevent further channel incorporation, the cis compartment was flushed extensively with fresh buffer solution. The current was converted to voltage and amplified by either an Axopatch 200A (Axon Instruments, Foster City, CA) or a Dagan 3900A patch clamp amplifier (Dagan Instruments, Minneapolis). The signal was filtered at less than 3/8 the sampling rate, which was typically 64,000 samples/sec, using a Frequency Devices (Haverhill, MA) low pass Bessel filter. Unless stated otherwise, the data was digitized using a National Instruments (Austin, TX) AT-MIO-16-X 16-bit board using programs written in lab windows/CVI (National Instruments). The bilayer apparatus was shielded from electric and magnetic noise sources using a μ-metal box (Amuneal, Philadelphia).
For most of our experiments we used polyuridylic acid (poly[U]) because this homo-polynucleotide has minimal secondary structure or base pairing (24) that might interfere with translocation of the polymer. Fractions of the poly[U] (Sigma), prepared with polynucleotide phosphorylase, were size selected by elution from agarose after fractionation by electrophoresis in the presence of formaldehyde. All DNA was synthesized using standard phosphoramidite methods. Although the sequence of the 150-nt DNA (CTCACCTATC CTTCCACTCA TTTTCCTTAA CCATTTCATT CACCCATCTC TTCACTCCAT CTATCACCTC CATACATACC CTCCATATTA CACTCCCATC ACTATCATTA TCTACATCCA TTACATCACT ACTCCTCACA CTACCATACC) was designed to minimize base pairing expected in the nondenaturing conditions we used, this polymer exhibited a larger proportion of long-lived blockades than did the homopolymers. Many of these blockades required clearing by brief polarity reversal. The 120-nt DNA for competitive PCR contained the same sequence as the 150-nt molecule but lacked residues 97–126. Double-stranded DNA, designed to be amplified by PCR using the same primers as the 150-nt single-stranded DNA, was made by mixing and annealing a synthetic 100-nt sense strand with its 100-nt antisense strand. The 100-nt sense strand contained the same sequence as the 150-nt molecule but lacked residues 49–98.
PCR Analysis and Blockade Counts.
Competitive PCR analysis (25) was performed after amplification (26) optimized to avoid the plateau phase. To prevent DNA loss by adsorption to the trans chamber walls, 55 μg/ml of carrier poly[U] was added to the trans chamber when DNA was to be harvested for PCR analysis. The PCR products were resolved on a 15% (total monomer) acrylamide gel and stained with SYBR Green I (Molecular Probes) for densitometry. In experiments with 1–8 channels, the number of blockades in peak 2 + peak 3 was determined by counting individual blockades during several 5-min sampling periods throughout the experiment; in the experiment with 40 channels, the number of blockades was estimated by multiplying (peak 2 + peak 3 rate observed with one channel) × (number of channels in the membrane) × (time of experimental voltage application).
In the absence of polymer, applying a potential of −120 mV (cis side negative) resulted in single channel currents that were free of transients (Fig. 1). Following the addition of poly[U] to the cis chamber, numerous short-lived current blockades occurred. Typically, the magnitude of the blockades was large (the current was reduced by 85–100%) and, depending on polymer length, the blockades lasted from several hundred to several thousand microseconds. For a given polymer size, the total number of transient blockades was directly proportional to polymer molar concentration (not shown). Virtually no blockades were observed in the absence of poly[U] (Fig. 1) or if the potential across the bilayer was reversed (not shown). Occasionally, after adding polymer, long-lived blockades of several seconds or more occurred which could be cleared by briefly reducing the voltage to 0, or by reversing the polarity of the potential. We assume that these indefinitely long blockages were produced when a polynucleotide entered but could not completely traverse the channel because of entangling secondary structure. The millisecond time scale blockades were also seen in similar experiments with poly[A], poly[C], poly[dT], poly[dC], as well as with single-stranded synthetic DNA composed of 150 nt of poly[dA, dT, dC] (see Materials and Methods).
If the blockades were caused by the transport of extended linear polynucleotides through the channel, the blockade lifetimes should be proportional to the length of the polymer and inversely proportional to the applied potential. A histogram of the current blockade lifetimes for 210-nt-long poly[U] shows that the blockades cluster into three distinct peaks (Fig. 2). Similar histograms for other size selected poly[U]s demonstrated that the mean lifetimes for peaks 2 and 3 were related to polymer length and inversely related to the applied potential (Fig. 3). On the other hand, the numerous fast blockades in peak 1 were independent of polymer length (Fig. 3a) or applied potential. We attribute these fast peak 1 blockades to polymers that collided with the channel, or partially entered but failed to traverse the channel. Thus, only the blockades in peaks 2 and 3, whose lifetimes were proportional to polymer length, appeared to be the result of polymers that traversed the channel.
Although the length dependency for peaks 2 and 3 was consistent with the notion that a polynucleotide traversed the channel as an extended linear chain, we were surprised that passage through the channel of relatively monodisperse, unbranched polymers would produce blockades whose lifetimes fell under two distinct peaks that contained approximately equal numbers of events. An explanation for the presence of these two peaks is that the characteristic times for polymer transit in the 3′ to 5′ direction may differ from that in the 5′ to 3′ direction. If true, the physical basis for such a difference remains to be explained.
The 2.6-nm diameter of the α-hemolysin channel is apparently too narrow to allow passage of double-stranded polynucleotides because blunt-ended double-stranded DNA produced only the rapid blockades similar to peak 1 events. We also tested mixtures containing equal concentrations of diverse length poly[A] with poly[U] or diverse length poly[dC] with poly[dG]. Such preparations would contain many base paired regions surrounded by unpaired single-stranded ends that might enter the channels but stop at a region of paired bases. As expected, these preparations produced indefinitely long blockades that required voltage polarity reversal to be cleared.
To demonstrate that blockades were attributable to DNA traversing the channels, we performed experiments to compare the number of blockades observed in peaks 2 and 3 with the number of single-stranded molecules that actually traversed the bilayer from the cis to the trans chamber. Taking advantage of our observation that double-stranded DNA could not traverse the α-hemolysin channel, we used double-stranded DNA as an internal control for inadvertent contamination of the trans chamber. The results (Table 1 and Fig. 4) showed that the amount of single-stranded DNA in the trans chamber approximated the amount we estimated should have accumulated. The single-stranded DNA found in the trans chamber could not be the result of contamination by aerosol or leaks from the cis chamber because such contamination would have yielded approximately the same ratio of single-stranded 150-nt DNA to double-stranded 100-nt DNA in the trans chamber as in the cis chamber. This was clearly not the case (Fig. 4, lanes 1, 2). Because the number of single-stranded molecules that accumulated in the trans chamber depended on the number of channels in the membrane and was proportional to the total number of peak 2 and peak 3 blockades, significant movement of single-stranded DNA through the lipid bilayer itself, rather than through the channels, could be ruled out.
While we have not yet established the quantitative relation between polymer molar concentration and blockade rate for a wide range of polymer sizes, we have observed that the number of blockades/minute produced by short (≈200 nt) polymers was significantly greater than the number produced by equimolar concentrations of longer (≈1000 nt) polymers. These observations suggested that the blockade rate could be used as an especially sensitive monitor of polynucleotide hydrolysis. For example, hydrolysis of long polymers by ribonuclease would increase blockade rate by simultaneously increasing the polymer molar concentration and decreasing the polymers' sizes. Indeed, upon addition of ribonuclease A to a cis compartment containing poly[U] there was a striking increase in blockade rate until the polymer fragment sizes fell below the temporal resolution of the instrumentation, whereupon the detected blockade rate decreased until no further blockades were observed (Fig. 5). Upon addition of fresh poly[U] to the cis chamber, a burst of blockades was again observed (not shown), demonstrating that the channel was still capable of detecting polymer induced blockades and that active ribonuclease remained in the cis chamber. Ribonuclease had no effect when poly[U] was replaced by poly[A], which is not a substrate for ribonuclease A. Thus, the initial increase in blockade rate cannot be attributed to the action of RNase on the bilayer or the channel. We conclude that analysis of blockade rate can provide a novel and rapid measure of enzymatic activity.
The discovery that a polynucleotide can easily be drawn through a single, well-defined channel provides a simple system for measuring the driven motion of polymers through constraints, a basic problem underpinning many biological processes, as well as gel electrophoresis (27). Recent theories of polymer translocation through a single membrane pore (28) and through multiple pores (22) could easily be tested in this system.
The realization that single channel measurements can be used to determine the lengths of individual RNA or DNA chains suggests that other characteristics of a polynucleotide could also be measured as the molecule traverses the channel. In principle, a single purine or pyrimidine nucleotide passing through the limiting aperture of a channel should block the ionic current in a manner that reflects the molecular size and chemical properties of each nucleotide in a polynucleotide. If so, it may be possible to use single channel recordings to directly determine the sequence of a polynucleotide. For this to be possible, at least five conditions must be met: each nucleotide must produce a characteristic transient current blockage,¶ the limiting aperture of the channel must have appropriate dimensions to reflect the presence of only one nucleotide at a time, the time resolution of ionic flow measurement must exceed the rate of nucleotide movement through the channel, backward movement of the polynucleotide must be minimal, and the channel and membrane must be sufficiently robust to withstand whatever temperature and chemical treatments are required to eliminate interference from polynucleotide secondary structure.
We thank H. Bayley for the generous gift of α-hemolysin and H. Berg, J. Golovchenko, and D. Nelson for their encouragement and suggestions. We also thank A. Schroder, R. Li, and S. Lee for technical assistance. This research was supported by grants from the National Aeronautics and Space Administration (D.W.D.), the National Science Foundation (D.B.), the National Institutes of Health (D.W.D.), and a National Academy of Sciences/National Research Council Research Associateship (J.J.K.).
↵ To whom reprint requests should be addressed. e-mail: dbranton{at}harvard.edu.
Daniel Branton
The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
↵ In closely related experiments, F. Roth, R. Baldarelli, and G. M. Church have modeled the movement of DNA through the pore formed by the LamB receptor. Their model shows that DNA can cause conductance changes that should be sensitive to the structure of the different bases (personal communication).
- Accepted September 5, 1996.
- Copyright © 1996, The National Academy of Sciences of the USA
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- Location of a Constriction in the Lumen of a Transmembrane Pore by Targeted Covalent Attachment of Polymer Molecules
- Automation for Genomics, Part Two: Sequencers, Microarrays, and Future Trends
- Reversal of charge selectivity in transmembrane protein pores by using noncovalent molecular adapters
- Rapid nanopore discrimination between single polynucleotide molecules
- Conformational dynamics of vesicles
- Passive entry of a DNA molecule into a small pore
- Identification and characterization of a cell membrane nucleic acid channel
- DNA Translocation Across Planar Bilayers Containing Bacillus subtilis Ion Channels
- Kinetics and mechanism of DNA uptake into the cell nucleus
- Kinetics of duplex formation for individual DNA strands within a single protein nanopore
- Partitioning of a polymer into a nanoscopic protein pore obeys a simple scaling law


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There are days in the mountains that have you hiding behind rocks to get out of the relentless wind. Days when you tug your hood over your face to shield it from blasts of hail. Days when you think: "Where did all this begin?"
Shiny Clouds by Ketsa, Butterfly/Kid On The Mountain and Lark In The Morning by Sláinte, We Were Never Meant To Live Here by Chris Zabriske, Mantra by Jahzarr and A Storm At Eilan Mor, Epoch and Upbeat by Jon Luc Hefferman.
Thanks very much to Bill Whiteford. Editing help from Huw Williams. Mountain is sponsored by Alpkit.
You can vote for Mountain in the Listener Choice category of the British Podcast Awards. Thank you.


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Join us this weekend as we bring you same day coverage of all the action from The US Open Snowboarding Championships, We'll have stories and photos as athletes vie for $130,000 in prize money
Over 500 athletes representing 20 countries, compete at The U.S. Open each year. Current elite pros such as Shannon Dunn, Ross Powers, Nicola Thost, and Drew Nielson, mixed with old-school legends on the comeback trail, and complete newcomers from the most remote places on the planet, all surface at the Open to compete on an even platform. In four days, heroes are made in Halfpipe, Big Air, Duel Slalom, and Boardercross.
Tune in to Snowboarding-Online.com each evening for total coverage of the day's events.
Event Schedule:
Thursday, March 16: Men's and Women's Halfpipe Qualifiers
Friday, March 17: Men's and Women's Duel Slalom CompetitionEvening Big Air Competition (under the lights)
Saturday, March 18: Men's and Women's Halfpipe Competition
Sunday, March 19: Men's and Women's Boardercross Competition and the all new Junior Halfpipe Competition


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Recording artiste Tommy Lee Sparta is disassociating himself from an individual, who he claims is attempting to mislead the public that he is part of his Sparta unit.
According to Tommy Lee Sparta, the artiste who goes by the name Stamma Gramma Sparta, has been using his likeness and vocals in a new song about scamming without his consent.
"I hear I have a look-alike using the name Sparta, saying we are friends and singing about scamming. I do not know this bredda at all. Never see him or talk to him yet. I believe in working for what you want. I do not support any fraud of innocent people. He is jus looking a lil' hype. He is not a member of my camp," Tommy Lee Sparta told THE STAR in an exclusive interview.
The song, which has been labelled the 'scammer anthem', has been racking up views on youtube with persons lashing out at Tommy Lee Sparta for condoning a criminal act.
"Mi can only talk fi miself. A nuh my ting that, and mi no want people a say them things deh bout mi. Me nah run down no hype so mi nuh interested to brand myself as a scammer. Everybody different you zi mi, some man good wid certain tings, some man nuh need it. Seven brother, seven different mind."
Tommy Lee Sparta says he is totally opposed to the illegal practice of scamming and wants no affiliation to this individual whom he has never met.
"Him use mi voice and mi intro fe mi song, Maniac. When mi seh 'Sparta...Yow', a dat him a use, mi hear. People a tink now say a Sparta newborn. Why him take set on me, I don't know him from nowhere. I believe that everybody deserves to get a buss so I wish him the best but leave me out of your argument," he continued.
According to Heavy D, Tommy Lee's manager, the new artiste appears to have an obsession with Tommy Lee.
"If he is the same person that is emailing me about rhythms that he built for Tommy, then he seems to be crazy and obsessed. This is pretty much stalking. I have been pestered via email, whatsapp and BBM. The person said they want to build website, design for Tommy Lee and sell his music. He called me sounding very disturbed and saying that we diss him, because Tommy Lee is voicing for everybody except him. Based on his approach, I don't want to have anything to do with him."
He continued, "I even got a call that he is angry that a producer stole his beat and voiced Tommy Lee on it and he wants credit. It would appear that he is filled with vendetta and using a gimmick with Tommy Lee's name to push it."
Arrangements are being made with You- Tube to have these videos with Tommy Lee's vocals permanently blocked from the site.
In the meantime, Tommy Lee Sparta has been receiving rave reviews for his latest single, Spartan Angels, which features Tabeta Cshae.


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West Branch
West Branch Meeting Room A
Thursday, February 15 2:00 pm to 3:00 pm
Emma Woodhouse is young, headstrong, wealthy and very much interested in love. Not being in love herself, but helping others find love. After successfully matching her governess with a local widower, Emma sets her sights on new friend Harriet, determined to match her with the perfect man. Everything goes awry when secrets are revealed and intentions are misread - and Emma is in the middle of it all. Revisit the classic love story Emma from Jane Austen. Book Chat is a librarian-led book club meeting on the third Thursday of the month. Meet with your fellow book lovers for an hour of discussion. Reading the book beforehand is not necessary.


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Sea Sure Swim School
Our Sea Sure Swim School project aims to reduce drowning deaths in South Australia.
The National Coastal Safety Report indicates that only 38% of Australians can swim further than 30m in the ocean without stopping. It also says that swimming accounts for 33% of all drowning deaths. Our statistics indicate that senior Australians, Indigenous Australians, youth and people from Culturally & Linguistically Diverse (CALD) backgrounds are over-represented in drowning and rescue statistics.
With 9.2 million beach visitations taking place in South Australia each year and 51% of all SA residents swimming at our beaches during the summer months, we know that preparing the community for coastal conditions is a core priority for drowning prevention.
With this in mind Surf Life Saving South Australia would like to introduce the Sea Sure Swim School. It involves inviting public access to free, social and fitness opportunities whilst improving coastal safety literacy and increasing bonds between lifesavers and the community.
Project Details:
Course: 5 week program of FREE ocean swimming lessons
Days: Mondays & Wednesdays
Age: 18+
Location: Glenelg Beach
Dates: TBC
Times: 5.30pm OR 6.30pm
Duration: 1 hour lesson, including water safety education
Capacity: 30 participants per hour time slot (10 per group)
The swimming lessons will be delivered by accredited swimming instructors and aims to improve the open water swimming ability of community members.
The benefits to the community include:
Increased public safety at SA beaches
Improved physical & mental health outcomes for participants
Increased access to safe and affordable fitness activities at the beach
Improved social connection for marginalised community members
Increased diversity in participants in Surf Life Saving.
For more information on the Sea Sure Swim School contact
Reception at Surf Life Saving SA on (08) 8354 6900


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Photo: Michael Heiman/2011 Getty Images
mr. 3000
Bidding on Derek Jeter Foul Ball Reached $17,392 — and Didn't Meet the Reserve
You'll recall that a couple weeks back, a foul ball that Derek Jeter hit during the at-bat in which he got his 3,000th hit went on the auction block. You may also recall that, as of last Monday, bidding had already reached $10,000. Well, bidding ended this weekend, and in all, the ball received fourteen bids — the highest of which was for $17,392. But here's the thing: That bid — which, we'll remind you, is for a foul ball — didn't meet the seller's reserve price. Somewhere, non-greedy souvenir-snagger Christian Lopez is crying, just a little. [Sports Collectors Daily via darrenrovell/Twitter]


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Get on board at Property Central
Property Central
Aerial view of the City campus as viewed in Property Central.
More informationFor more information, email: firstname.lastname@example.com
- What is Property Central?
- Property Central: at work with other online systems
- Property Central FAQs
- More information
What is Property Central?
Property Central is a web portal system that is an industry-standard, GIS (Geographical Information System) application and a centralised, user-friendly repository of all onshore RMIT campus building plans and facilities information.
Staff can efficiently access information on RMIT's physical resources - building, space and campus information and information specific to the University's Colleges, Schools and Groups via the web.
Campus maps and building floor plans can also be modified to suit user requirements. Notations can be added and a wide range of information can also be added or removed, like Usable Floor Area (UFA), room type and the School or Group that uses the space.
Generate a campus map
You can view an aerial map of the selected campus, hone in on a building, and view details of that buildings individual floors and rooms on that floor.
Generate a room enquiry
Bring up detailed information about any room on any of RMIT's onshore campuses and generate a floor plan with a pointer to the room that is being searched for.
Generate space reports
Run a variety of reports, which include listings of different classroom types in a building or on a campus.
Property Central: at work with other online systems
Property Central can also be used in conjunction with Property Services' other online systems.
Web Room Booking System
If you need to make a room booking for a meeting, makeup class or event, search for an appropriate venue in Property Central and then book it online using the Web Room Booking System (WRBS).
Project Request System
If you need to submit a project request - that ranges from requests to have a room painted to major renovation works – mark up a floor plan in Property Central with details of the required work and submit it as an attachment to the project request you enter into the online Project Request System.
If you need to submit a maintenance or service request, log into Archibus with your e-number and password..
Property Central FAQs
- 1. How do I log in to Property Central?
- 2. How will Property Central benefit me?
- 3. Can I download a campus map and floor plan and how do I do it?
- 4. What if I find an error in Property Central?
- 5. What new feature are going to be added to Property Central?
1. How do I log in to Property Central?
All staff and students have general access to Property Central, which does not require a login.
Should you wish to obtain a higher level of access, you need to login, using your E-number and your NDS password. The higher level of access will enable you to retrieve staff location details.
2. How will Property Central benefit me?
Property Central will enable you to perform a number of functions, including:
- Location of a staff member
- Location of a space: a meeting room or classroom
- Finding and viewing a classroom that has been allocated to you for one or more of your classes.
Resources staff will find Property Central useful to assess space that has been allocated to their Group or School.
3. Can I download a campus map and floor plan and how do I do it?
Yes, you can download a campus map or floor plan. Select the "Print" function from the "File" menu and then select the "CutePDF Writer" option from the menu of printer options.
You will them get a Windows prompt to print the campus map or floor plan. Select "Print" and then you will get the "Save As" dialogue box, in which you will name the file - which will automatically save in PDF format - and save it to your selected location.
4. What if I find an error in Property Central?
Please contact firstname.lastname@example.com and provide details of the error.
Examples of errors include:
- Staff member in wrong location
- Missing walls in a floor plan
- Incorrect department code.
5. What new features are going to be added to Property Central?
The following features will be added to Property Central:
- Documents relating to building changes
- Photographs of teaching spaces and their layouts
- Locations of rubbish bins
- Environmental information, including building energy and water efficiency data.
More information
For more information, please email email@example.com


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Crap. My hair. That was my first thought (followed a few days later by weight loss here I come -but don't be fooled you actually put weight on). Crap.
So after the first chemo I became hair obsessed. Head hair, leg hair, arm hair, face hair (yes face hair -who knew it was actually that hairy), eyebrows, eyelashes, lady garden hair, nose hair -you name it i was obsessed with it.
In fact one thing I wasn't told about was nose hair. When people say chemo, you generally associate it with throwing up and loosing the hair on your head, no one ever mentioned anything about bloody nose hair. So when mine fell out and all of a sudden it was freeeeezing cold and streaming I convinced myself that I had nose Cancer. Yep nose Cancer. It was the only logical explanation, that was until my next clinic appointment and the nurse casually said
"Oh yes that's normal it's just your nose hair falling out" brilliant, thanks for that.
Then the lady garden hair fell out - and Arron gave me the loving nick name "Sconner." This wasn't really a big deal, neither was the arm pit hair or leg hair, in fact it was rather nice not having to Veet/shave etc.
Im not going to write about loosing my head hair as there are plenty of awesome blogs about that. I will say that I found loosing my eyebrows devastating, and hated the generic "Cancer face" - see below -
I then adopted the even more
Loving Nick name of "Sconner Face."
I wish I'd had them tattoo'd on. Yes you can draw them on. Yes some of the products available are great. Yes you can buy stencils (actually amazing and probably one of my best chemo presents I received). But it takes ages. Making sure they're symmetrical, not too thick, not too Scouse brow, level, not too obviously drawn on. There's no such thing as quickly getting ready, it's a bloody nightmare, and you have good eyebrow days and bad eyebrow days. This is probably a good day -
Clearly I have no eyelashes but eyebrows have been drawn on reasonably well here. When I had surgery I asked the nurse 2 things - could I keep my knickers on (only so I would know if I'd wet myself during the 4hr op) and if I could keep my eyebrows drawn on (I don't think she'd ever been asked that). Not sure what happened but I woke up looking like the dad from American Pie-
So then chemo finished (yay) and hair started growing back whilst waiting for surgery. Which sounds good but 2 things -
1. It means that the chemo is pretty much out of your system (crap, that means it could start growing/spreading)
2. It grows back with a vengeance (well,everywhere EXCEPT your head).
My eyebrows sprouted overnight but now grow outwards, and again not evenly, getting my HD brows was genuinely one of the happiest days ive had in ages -
But unfortunately for me it's not the 70's and im not a porn star so my 70's bush takes a lot of work (bring back sconner Aimee), and it's also poker straight, as in ive used GHD's straight and I can assure you that I haven't -no pictures fortunately.
Im also sick to death of shaving my legs and arm pits, no matter what you use nothing, and I mean nothing gets it as smooth as chemo does....


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Understanding Earnings Inequality in Appalachia: Skill Upgrading versus Rising Returns to Skill
Understanding Earnings Inequality in Appalachia: Skill Upgrading versus Rising Returns to Skill - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.
The Appalachian region is one of the most persistently poor areas of the United States. A focal explanation for the weak economic performance over the years is the fact that Appalachia has long lagged behind other regions in terms of the supply of skilled workers, particularly those with higher levels of education attainment, and this lack of skill has perpetuated poverty in the region. In recent decades, however, residents of Appalachia have begun to narrow the gap in education attainment. To what extent this relative skill upgrading in Appalachia has translated into higher wages and reduced wage inequality across regions of the country depends on changes in the relative returns to skill. Knowledge of how regional differences in skill levels and returns to skill translate into regional differentials in economic inequality and development is needed for a better understanding of widening inequality. This report uses data from the 1980-2000 Integrated Public Use Microdata Samples (IPUMS) of the Decennial Census to decompose changes in the wage levels and distributions of men and women within and outside Appalachia over the past two decades. Evidence indicates that while still lagging behind the United States as a whole, the Appalachian region has shown some social and economic convergence toward the United States during the last decade. The convergence appears to be primarily in the direction of decreased income inequality and high school graduation rates. Income growth in Appalachia has generally kept pace with the United States, but average income levels are still below those of the rest of the nation. (Contains 1 footnote, 13 figures and 15 tables.)
Descriptors: Salary Wage Differentials, Poverty Areas, Rural Areas, Outcomes of Education, Differences, Regional Characteristics, Rural Urban Differences, Educational Attainment, Gender Differences
University of Kentucky Center for Poverty Research. 302D Mathews Building, Lexington, KY 40506. Tel: 859-257-7641; Fax: 859-257-6959; e-mail: ukcpr[at]uky.edu, Web site: http://www.ukcpr.org
Autor: University of Kentucky Center for Poverty Research
Fuente: https://eric.ed.gov/?q=a&ft=on&ff1=dtySince_1992&pg=5302&id=ED505044


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Psychology Press
312 pages
In contrast to other disciplines, social psychology has been slow in responding to the questions posed by the issue of ethnicity. The Social Psychology of Ethnic Identity demonstrates the important contribution that psychology can make.
The central aim of this book is to show, on the one hand, that social psychology can be used to develop a better understanding of ethnicity and, on the other hand, that increased attention to ethnicity can benefit social psychology, filling in theoretical and empirical gaps. Based on recent research, The Social Psychology of Ethnic Identity brings an original approach to subjects such as:
* ethnic minority identity: place, space and time
* hyphenated identities and hybridity
* self-descriptions and the ethnic self.
The combination of diverse approaches to this burgeoning field will be of interest to social psychologists as well as those interested in issues of identity, ethnicity and migration.
Social Psychologies. Social Identity. Ethnicity. Ethnic Minority Identity: Place, Space and Time. Ethnic Group Essentialism. Hyphenated Identities and Hybridity. Self-descriptions and the Ethnic Self. Conclusion: Towards Better Understanding.
The European Monographs in Social Psychology series is published in conjunction with the European Association of Social Psychology, to support authored books for advanced students covering social topics that promote a European intellectual perspective to the rest of the world.
The aim of this series is to publish and promote the highest quality of writing in European social psychology, and provide an outlet for new research in the field. At a time when cultural and political frontiers in Europe are being redrawn, there could hardly be a more appropriate time to promote a distinctively European intellectual perspective to the rest of the world. The editor and the editorial board encourage publications which approach social psychology from a wide range of theoretical perspectives and whose content may be applied, theoretical or empirical.


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Translation Project Management: Asking the Right Questions
June 23, 2017
Localization project managers have a clear understanding of the tasks and requirements of translation projects. However, clients, especially those new to localization, may not fully understand what information they need to provide and which questions they need to answer before a project begins. To avoid making assumptions, which can lead to miscommunications with your clients, I have put together some basic questions localization project managers should ask before beginning a translation project. This list of questions will also help clients know what information is important to provide to their translation agency.
This blog will focus on the general questions you will want to ask for any type of translation request. But, there are also questions specific to the types of content you are translating: website, document, software, etc., which I will cover in future blogs. This is also not a complete list, rather a starting point. You may have many more questions depending on the project requirements.
Below is a list of questions you should ask before sending your project kick-off email:
What are the source and target languages?
It is important to be clear about this before starting the project to be sure you have the appropriate resources available.
Where is your target audience located?
This is required so you can be sure you are translating into the correct dialect. For example, are you targeting Spanish speakers in Spain or Latin America? Is your French website aimed at French speakers in France or Canada? Identifying your target audiences will ensure you are creating culturally appropriate content and your message is being effectively received.
Do you have onsite reviewers we should meet with before the project starts?
If these reviewers will play a significant role in any phase of the project, it's best to communicate with them from the start about expectations and roles.
Do you have any style guides or translation memories from similar localization projects?
Style guides and translation memories will help translators create content that is consistent with a client's messaging, tone and style.
In which type of file format would you like to receive the final translated content?
As an example: if a client needs the translated content for a document project in exactly the same format of the source document, like a PDF, they should provide editable files so the translation teams can work in the document and send the translated PDF back to the client.
The questions mentioned in this blog are just the starting point for what you should ask clients. You can use your professional experience managing multilingual projects to build on this list and ask questions more specific to the client, languages, locales and project type. In my upcoming blogs I will cover the questions to ask for document, website and software translation projects.
Additional Resources for Localization Project Management
You may gain further insight into project management best practices, global e-business, global SEO, translation, localization and related topics by reviewing some previous blogs written by GPI:
Please feel free to contact GPI at email@example.com with any questions about our translation services. Also let us know if you have any interesting blog topics you would like us to cover in future blogs. You may also request a complimentary Translation Quote for your projects as well.
- Category:
- Language Translation Facts
- Tags:
- project management
Cultural Customization: Part 1Website Localization Tips for the Arabic Market
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Ahmed Mossad El-Saba - Localization Project Manager
Ahmed is a native Arabic speaker from Egypt, Cairo. He has over 12 years' experience in the translation and localization field working as a Hebrew – Arabic translator and editor. He has also held positions for language Testing Engineer and Localization Project Manager. Ahmed has extensive experience in handling large volume translation projects for software and document localization. He has worked for a range of translation and localization groups and companies including Microsoft, Babylon, Saudisoft, as well as several governmental authorities. He holds a B.A. degree in Translation and Interpreting from Faculty of Alsun Ain Shams University and additionally is a certified Hebrew - Arabic translator with a High Translation professional diploma from the same faculty. He is a published author translating news articles and writing political analysis with contributions to an array of research centers and newspapers including AL Aharam, Beirut Political Centre, Aldiplomasy Magazine, and Lindro Italian News, to name a few. In his free time Ahmed likes swimming, shooting and reading about new ideas.


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Sunday Morning Newpaper Reading Music… Enjoy.
Almost 40 minutes of mellow music to get you through a quiet Sunday morning… "Andreas Vollenweider"
|Andreas Vollenweider – DrmzSpace Radio Volume 6 – Andreas Vollenweider
|Found at bee mp3 search engine
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Critical Linking: December 11th, 2014
Understand the grammar in your favorite carols.
This was unexpectedly interesting.
An illustration of Winnie the Pooh has been sold at auction for over £300,000. EH Shepard's famous ink drawing of the bear playing Poohsticks with Piglet and Christopher Robin was published in 1928, and sold for £314,500. A pencil drawing of the same scene sold at Sotheby's in 2013 for £58,750.
If I were to drop a half-million bucks on a Winnie the Pooh illustration, it would be a pooh sticks illustration.
Eleanor & Park author Rainbow Rowell announced her next book, and it will please fans of her Fangirl. Rowell explained her next book will be titled Carry On, and it will be about Simon Snow.
If you are an Eleanor & Park fan, then this is very exciting news. And if you aren't, let me tell you that this could be a big deal.
We run a pretty sweet little bookish Instagram account, if we do say so ourselves (and we do). Come check it out.