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1. John Carmack joins the Oculus team to work on the software for The Rift
John Carmack is a co-founder to the id software, and is most known for his programming work in the quake series. He has recently joined Oculus as Chief Tech Officer, where they have been focused on virtual reality technology. The goal for Oculus over the past year has been to develop a new type of hardware, where the user would be engulfed in a virtual reality while playing their game.
According to Carmack in an interview at QuakeCon in 2012 the prototype they are selling at the moment, is not meant for consumers. The project is still very much in the developmental stage and those at Oculus are hoping to get developers to look at their work and give feedback. In time after they work out all of the kinks, such as developing better software to support it and having more than one game to play on it, then it will be consumer ready. However, they recommend to those who want to support their project, wait until it would be worth the cost when it is software supported.
2. LiveScript, Julia and Elixir: Three Languages To Watch
Livescript is a JavaScript and CoffeeScript compatible language that is meant to be more user friendly. Livescript 1.2 was recently released, and is meant to be used at any point a person would use Java.
Julia is another language, more specifically meant for science, since it is more expressive with a larger library than most other languages that focuses on scientific computing tasks. Julia is not only a higher performance language, but is also faster than other languages.
Elixir is a modern version of Erlang VM, taking out what did not work in Erlang and keeping what did is a step forward to say the least. Some of Elixirs features are: list comprehensions, Metaprogramming via macros, and Protocol-based inheritance.
All of these more unheard of languages have massive potential, and have the purpose of making life easier for those who want to or need a language, but are unsure as to which is best for you. There are more than just the well-known and popularized languages such as JavaScript and Coffeescript.
3. A New Form of Terrorism: Hacker Terrorists
With the increasing dependency on the internet, a new form of terrorism is being achieved through hacking.
Michael Hayden, former NSA chief, calls these transparency groups "terrorists."
But there are two sides to the story. Some see releasing sensitive information, as Edward Snowden did, as dangerous to the US government. Others see the transparency groups as "hacktivists" trying to bring light to unknown government activity.
Hayden jumps to the conclusion that hackers will target The World Trade Centers. "So if they can't create great harm to dot-mil, who are they going after? Who for them are the World Trade Centers? The World Trade Centers, as they were for al-Qaida." Said Michael Hayden during his Washington speech given on Tuesday, assuming that hackers want to target the US government for malicious reasons.
Although the conclusion given is from assumption, there is real fear with the dependency on technology today. When it comes to banking and personal information being out there, it is a real threat that personal and government information could be used for harm. Real terrorists, not just transparency groups, could use hacking to access private information from the government and use it to cause harm.
4. Google glasses are potentially disruptive and could violate privacy, but do the risks out way the potential benefits?
Some of the many points brought up against Google glasses, are the safety concerns when driving. It was said by "Henry Jasny, Advocates for Highway and Auto Safety's general counsel. 'It will be just like cellphones, which became too ubiquitous and popular while driving."' (Jessica Meyers) Also thought to be a privacy issue when it comes to doctor patient confidentiality, yet it is also believed google glasses could help determine a diagnosis.
Another point brought up by police, is that although it could help with evidence, the video could be turned off and on leaving out context, leading to more troublesome than beneficial proof. There is also potential use for piracy when it comes to movies in the theaters. Many places are already beginning to ban Google glass from being used, even though there is not even a release date for the product.
Senate Commerce Committee Chairman Jay Rockefeller believes that no matter what is thought of now, it will take time before any policy can be made. This is probably for the best, since the only way to know the full scale of issues and benefits brought by Google glass will be known once the product is out and used by large number of the populace and not just the few who got a hold of the prototypes from Google.
5. Starting-Up your own business? Here are some tips
Good management is not the most important thing, companies who are just starting out often do not have the resources for it, and many big companies today started out very small with none at all. Although recently The Wall Street journal reported that cyber security was not going to be very big, Defense.net reported 9.5 million in funding. One thing to keep in mind is that most entrepreneurs often did not get in much legal trouble as teens, according to Arizona State University study.
However, if you are not looking to start a business, but instead invest in one, looking at the JOBS act and becoming an angel investor might just be the route for you. It is an easier way to get involved with much less risk than starting a business of your own.
6. New OS based on Bluetooth could revolutionize the way we live
Estimote is a company looking to promote their new product that brings the physical world reality into smart phones. If you were to walk around a museum, all you would have to do is look at your phone and the nearest art to you would be instantly described. If inside a store all that you were looking at would be in your phone, no need to keep track of what you want to get in the future, because your phone would do it for you.
Right now the technology is going to work using "motes" simple waterproof Bluetooth devices that would connect to smartphones, not just to track where you are, but with enough motes around the world or in a shop. A smartphone could map out the store that customer is in, also keeping track of products looked at and what part of the store the person is in.
With this technology, there is some privacy issues, since the ability to keep track of where a person is at all times is a little scary. Who exactly would be able to get a hold of this information would be important. However, it could revolutionize the way people shop, by eliminating buying at a store, it could just be a floor room and order over the phone while letting someone else handle the delivery, such as amazon. Possibly finding cheaper companies with the exact same product that is in front of you. Right now a package of 3 "motes" is available to developers for $99.


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TRENTON – NJDOT officials today announced that construction activity will resume this weekend along Interstate 78 from west of Ramsey Avenue (milepost 53.8) in Union Township to the Route 21 interchange (milepost 56.9) in Newark.
In December 2012, NJDOT restored all available Interstate 78 travel lanes in the project limits to provide maximum capacity through the winter season when major pavement construction is limited. As spring approaches and the asphalt plants reopen, work on the $72 million roadway rehabilitation project is set to gear back up.
Over the weekend, NJDOT's contractor, a joint-venture of Ferreira Construction and Crisdel Group, will resume long-term lane closures to allow for the completion of the full depth pavement replacement. The contractor will reduce I-78 westbound from five travel lanes to four – two Express lanes and two Local lanes – and begin reconstruction of the far right Local travel-lane and shoulder.
All existing travel lanes will be maintained on I-78 eastbound for now, as work continues to reconstruct the eastbound shoulder between mileposts 55.9 to 56.9. Long term single-lane closures will resume on the eastbound highway in the coming weeks.
Construction began in March 2012 on the major roadway rehabilitation with the highway being reconstructed in stages, requiring numerous traffic pattern changes and the closure of one travel lane in each direction at certain locations. A total of four of the five I-78 travel lanes will remain open in each direction during peak period hours throughout construction. Single and double-lane closures will be allowed during overnight and off-peak hours.
This is one of the busiest stretches of highway in New Jersey, with an average of 145,000 vehicles traveling it daily. The new pavement is designed to last up to 20 years. The project is scheduled to be completed in early 2014.
The precise timing of the work is subject to change due to weather or other factors. Motorists are encouraged to check www.511nj.org for real-time travel information.
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Join NJTODAY.NET's free Email List to receive occasional updates delivered right to your email address!

subset: commoncrawl


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Scroll down to view cottages in this area
Read on to discover some of the attractions you can enjoy whilst staying at one of our holiday cottages in Lincolnshire. Lincolnshire cottages offer the perfect accommodation for a getaway in this quiet, peaceful county of open spaces.The Wolds form Lincolnshire's spine, a line of chalk hills criss-crossed by lanes and river valleys, the highest area of land in eastern England between Yorkshire and Kent. This has been classified as an Area of Outstanding Natural Beauty and from our holiday cottages Lincolnshire, with its wealth of history and wildlife, can be explored. The county offers both pretty inland villages and seaside attractions. When it comes to self-catering Lincolnshire has some great family friendly beaches along its coast including Skegness, Mablethorpe and Cleethorpes.
A visit to the city of Lincoln, rising above the surrounding countryside on a cliff above the River Whitham, is recommended for those holidaying at any of our Lincolnshire cottages. Visible for miles around is the cathedral, with its mix of Norman and Gothic architecture. Much of the city's medieval character survives, particularly along the aptly named Steep Hill running up to the cathedral.
South of here lies the flat, watery landscape of the Fens and one of the last true English wildernesses, The Wash. Enjoy the wild and barren landscape at Frampton Marsh or visit the waders' lagoon at Freiston Shore, where in summer avocets, oystercatchers, redshanks and ringed plovers nest in their dozens. These are just a sample of the attractions which can be enjoyed by selecting one of our holiday cottages in Lincolnshire for your break. ... Read More »
Blyton Carr, nr. Gainsborough. View on map>
North Rauceby, nr. Sleaford. View on map>


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Colonial Spindle Pine Bed Frames
Delivery Info...
Local CustomersDelivered and assembled onsite. £10 local delivery. Customers are welcome to collect.
Nationwide Customers This product comes boxed flatpack for home assembly and is delivered via national courier. Standard courier delivery starts from £19.99-Please contact us for a quote.
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A Box of Sunshine
We have had a lot of gray days recently. On one such day, with rain threatening, I received a package filled with sunshine!
I was the winner of Garden Glimpses giveaway. Tammy was giving away all these cheerful items to mark her seventh(!) year blogging. Woot woot!
Tammy and her blog have blessed me in many ways throughout the years. Her writing has encouraged me and many posts have challenged me to be intentional in my day to day relationship with God.
One of the best surprises that came with this package was the fact that Tammy, who inspired me to start blogging, lives a mere half hour from my mom! As she put it, "God's way of putting a smile on my face."
Thank you for the box of sunshine Tammy and thank you for being a faith-full blogger.


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This hotest porn pic pictures has 1600 x 1067 119 kb Jpeg. Sexy lesbians licking pussy is a popular picture for wallpaper. If you have a problem about intellectual property, child pornography or immature images with any of these pictures, please send report email to webmaster at Farimg.com, we will remove it immediately.
- Next Hottest Babes


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THOMPSON-OKANAGAN - Nearly 50 communities set new high temperature records in B.C. yesterday, including more than a dozen in the Southern Interior.
Environment Canada is reporting new records in all corners of the province yesterday, April 18, including Kamloops, where a high of 30.5 C yesterday surpassed the previous record of 28.9 C, and Vernon, where a high of 26.4 C beat the previous record by 1.4 degrees. At 27.4 C, Kelowna beat the previous record of 26.1 C set in 1915 and Penticton, at 27.3 C, beat the previous record of 26.7 C.
Blue River, Clinton, Lillooet, Lytton, Merritt, Osoyoos, Princeton, Salmon Arm and Summerland also set new records.
Many of the records were broken by at least a few degrees yesterday, though in Puntzi Mountain and Fort Nelson, previous records were smashed by eight degrees. The oldest record to fall in B.C. was in the Victoria Harbour area, where the previous record of 17.8 Celsius was recorded in 1897.
The heat has been hitting more than just B.C. though. Yesterday 29 new records were also set in Alberta, another nine in Saskatchewan and three in Northwest Territories.
The high temperatures are expected to continue through the end of the week, with highs of 25 C to 31 C expected throughout the region.
Record highs for April 19 are in the mid-20s for the Okanagan and 28.3 C in Kamloops while records for April 20 are in the high-20s across the region. Kamloops will likely fall well short of the 31.1 C record for April 21, but with a high of 27 C in the forecast and records in the mid- to high-20s, Okanagan cities could set new records Thursday as well.
Seasonal normal high temperatures range from 18 C in Kamloops to 16 C in the Okanagan.
To contact a reporter for this story, email Jennifer Stahn or call 250-819-3723 or email the editor. You can also submit photos, videos or news tips to the newsroom and be entered to win a monthly prize draw.
We welcome your comments and opinions on our stories but play nice. We won't censor or delete comments unless they contain off-topic statements or links, unnecessary vulgarity, false facts, spam or obviously fake profiles. If you have any concerns about what you see in comments, email the editor in the link above.


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Instructions from Mimi "Write a dating profile for one of your past finished projects"
Are you feeling blue? Do you need cheering up?
I am seeking a companion.
I'm colourful and affectionate, my antecedents are noble. but I'm no good alone. I am an excellent travel companion whatever the weather. I can be your arm candy (well substitute shoulders for arm), will keep you warm and cheer you up on a dull day, I seek a mutually beneficial partnership. Without you I just hang around
Name - Color Affection
Age - 12 months
Origins - Countess Ablaze Dr Donegal
(slightly pretentious) Interests - Classical History
There - I said I would be perfect for you
But I can't promise I will not find other partners equally well matched... a coat perhaps or jacket...


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With Movember, a mustache growing initiative that occurs in November, underway, men should consult the 'To Stache or Not to Stache' infographic. In recent years, Movember was started as a means of raising awareness and money for prostate cancer while also giving men an excuse to sport some facial stubble. Learn how much it costs to maintain a handsome patch of facial hair, how much time you've been devoting to keep it looking dapper and the genres of scruff available.
According to the To Stache or Not to Stache infographic, the mustache comprises almost 5% of the total shaving area and those who adorn the iconic mo' on average earn more than clean-shaven men. On average shaving takes about 3 minutes per day, which puts this daily activities cost at about $1 in time. Whatever style your choose, be it fu manchu, handlebar or imperial, this graphic will guide you on the path to keeping your 'stache looking polished until the end of the month.
Fees of Facial Hair Visuals
More Stats +/-
Hipster Laptop Stickers
Gamer-Inspired Accessories
Facial Hair-Sporting Tumblers
Mustache-Shaped Hair Accessories
Hairy Automobile Accessories
The 'To Stache or Not to Stache' Infographic Examines Move
- By: Erin KirkpatrickNov 5, 2012
References: ddesignerr
References: ddesignerr


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Journal of Biomedicine and Biotechnology
Volume 2008, Article ID 375620, 7 pages
Infectomic Analysis of Gene Expression Profiles of Human Brain Microvascular Endothelial Cells Infected with Cryptococcus neoformans
1Divisions of Hematology-Oncology and Infectious Diseases, Saban Research Institute of Childrens Hospital Los Angeles, Keck School of Medicine, University of Southern California, Los Angeles, CA 90027, USA
2HRL Laboratories, LLC, Malibu, CA 90265, USA
3Department of Life Science, Fu Jen Catholic University, Hsinchuang, Taipei Country 24205, Taiwan
Received 12 August 2007; Accepted 17 December 2007
Academic Editor: Daniel Howard
Copyright © 2008 Ambrose Jong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
In order to dissect the pathogenesis of Cryptococcus neoformans meningoencephalitis, a genomic survey of the changes in gene expression of human brain microvascular endothelial cells infected by C. neoformans was carried out in a time-course study. Principal component analysis (PCA) revealed significant fluctuations in the expression levels of different groups of genes during the pathogen-host interaction. Self-organizing map (SOM) analysis revealed that most genes were up- or downregulated 2 folds or more at least at one time point during the pathogen-host engagement. The microarray data were validated by Western blot analysis of a group of genes, including -actin, Bcl-x, CD47, Bax, Bad, and Bcl-2. Hierarchical cluster profile showed that 61 out of 66 listed interferon genes were changed at least at one time point. Similarly, the active responses in expression of MHC genes were detected at all stages of the interaction. Taken together, our infectomic approaches suggest that the host cells significantly change the gene profiles and also actively participate in immunoregulations of the central nervous system (CNS) during C. neoformans infection.
1. Introduction
The major challenge posed by infectious diseases is to holistically and integratively understand the fundamental issues of how infectious agents and human hosts interact during microbial infection. Host-microbe interactions in the pathogenesis of infectious diseases are dynamic and complex processes [1] which result in changes in whole genome expression profiles of microbial pathogens and their hosts. A new discipline called infectomics [1] became established when the recently developed high-throughput omic approaches and computational tools were combined with the conventional approaches for the study of infectious diseases. Infectomes are detailed maps of microbial infections, and the availability of whole genomes of many living organisms paves the way for their holistic and integrative study. Infectomics can be defined as the study of infectomes, which are encoded by genomes of microbes and their hosts. Microarray has been a powerful tool to monitor infectomes in microorganisms and their host responses during microbial infection.
Infection by Cryptococcus neoformans has increased considerably over the past few years [2–4]. Dehydrated haploid yeast or basidiospore of C. neoformans is the usual form of inhalation [3, 5]. The organisms are likely to spread hematogeneously to extrapulmonary tissues and show a remarkable propensity in spreading to the brain and meninges, where life-threatening meningoencephalitis develops [2, 6, 7]. In order to cause meningoencephalitis, C. neoformans must penetrate the blood-brain barrier (BBB), which is a barrier between blood circulation and the brain parenchyma. BBB mainly consists of brain microvascular endothelial cells (BMECs), which are responsible for maintaining the biochemical homeostasis within the central nervous system (CNS) [8–10]. BMEC has been established as an in vitro cell culture model for dissecting the underlying mechanism(s) whereby C. neoformans crosses the BBB. We have recently demonstrated that C. neoformans are able to alter the cytoskeleton of human brain microvascular endothelial cells (HBMECs) [11]. We have also identified and characterized a C. neoformans capsule gene, CPS1 [12]. This demonstrated that CPS1 encodes hyaluronic acid synthase. The above information suggested that C. neoformans hyaluronic acid (HA) plays a role as an adhesion molecule during the yeast entry. It also suggested that host cell factors are required for C. neoformans HA-binding and the pathogen entry into HBMEC [12].
Like many other pathogens, C. neoformans may manipulate the host system to facilitate its invasion. The investigation of virulence of the pathogen C. neoformans and the study of the responses from HBMEC are equally important in the understanding of the complex invasion process. A more comprehensive knowledge of the interplay between the host and microbial pathogen at the levels of genome expression profiles is central to the understanding of the pathogenesis of infectious diseases. In order to dissect the pathogenesis of this disease, we have combined the infectomic approach with the in vitro model of the BBB to monitor gene expression profiles of HBMECs infected with C. neoformans. These studies provide global and useful information for building a comprehensive framework to interpret C. neoformans pathogenic processes.
2. Materials and Methods
2.1. Cultures of Yeasts and Human Brain Microvascular Endothelial Cells (HBMECs)
C. neoformans strain B3501 was used for this study [11]. Yeast cells were grown aerobically at 30°C in the rich YPD broth containing 1% yeast extracts, 2% peptone, and 2% dextrose. Cells were harvested at early log phase, washed with PBS, and resuspended in Ham-F12/M199 (1:1; v:v) and 5% heat inactivated fetal bovine serum (FBS) (experimental medium).
The HBMEC culture was prepared as described previously [11, 13]. Briefly, the HBMEC cultures were maintained in RPMI 1640 medium including 10% FBS and 10% NuSerum (BD Biosciences, Bedford, MA, USA) at 37°C in a humid atmosphere of 5% CO2 as described above. For the preparation of interactive cultures for microarray analysis, the HBMEC were grown in collagen-coated 24 well tissue culture plates (Costar Corp, Cambridge, MA, USA) until confluency. An inoculum of 106 yeast cells in 1 mL experimental medium was added. C. neoformans B3501 was incubated with HBMEC at 37°C and harvested at 0, 4, 8, 12, 16, 20, 24 hours. One-tenth of cell pellets were saved for Western blots and the rest were subjected to the RNA extraction for making the probes.
2.2. Preparation of the Biotinylated cRNA for Microarray
Total RNA was preparedusing TRIZOL reagent (Invitrogen, Calif, USA), and subjectedto isolation of poly(A)+ RNA using the Oligotex-dT30 mRNA purification kit (TaKaRa Shuzo Co., Kyoto, Japan) according to the manufacturer's instructions. The biotinylated cRNA probe was prepared using the RNA Transcript labeling kit (Enzo Biochem, Farmingdale, NY, USA) according to the manufacturer's instructions. The quality of the probe was first examined with 1% agarose gel, showing the bands between kb, peaking at around 0.75 kb. An aliquot of biotinylated cRNA probes was then examined by the Affymetirx test chips. Internal controls, G3PDH and -actin, on the test chip were measured to evaluate the biotinylated probes. Only high-quality cRNA probes were used to hybridize with DNA microarray HU95A chips (12,809 gene spots in ~1.64 cm2 filters). After hybridization, the HP GeneArray scanner was used to analyze the patterns of gene expression.
2.3. Microarray Analysis
Biotinylated cRNA probes were prepared from 10 g of poly(A)+ RNA. Hybridization and fluorescence detection were performed according to the manufacturer's instructions. Images were analyzed with GeneSpring, Genetrix softwares. Three clustering approaches were used in this study as follows. (a) Hierarchical clustering where the data points were organized in a phylogenetic tree in which the branch lengths represent the degree of similarity between the values. (b) Self-organizing maps (SOMs) that was a nonhierarchical clustering approach. Using this algorithm, gene expression data were transformed into vectors or coordinates in an -dimensional space, where equals the number of variables or time points. (c) Principal-component analysis (PCA) that was used to obtain a simplified visualization of entire datasets.
2.4. Western Blot Analysis
Protein concentration was determined by Bio-Rad protein assay and equal amounts of protein were used from different time point samples. SD-PAGE sample buffer (50 mM Tris-HCl pH 6.8, 10% -mercaptoethanol, 2% SD, 0.1% bromophenol blue, 10% glycerol) was added to the samples and they were boiled in a water bath for 10 minutes. 2 g of total cell extracts were separated on homogeneous 12.5% PhastGel (Phastsystem, Amersham Pharmacia Biotech, NJ, USA) with SDS buffer strips according to manufacturers instructions with subsequent transfer to PVDF membrane for 40 minutes. The membrane was blocked by 0.5% blocking solution (skim milk-based), incubated with anti-Bcl-2 antibody, anti-Bad antibody, anti-Bcl-x antibody, anti-Bax antibody (Transduction Laboratories, Calif, USA), anti-CD47 antibody (Lab Vision, Calif, USA), or anti--actin antibody (Chemicon Interna-tional, Calif) at 25°C followed by incubation with peroxidase-coupled secondary antibody (Kirkegaard Perry Laboratories, Md, USA) and detected by the ECL-enhanced chemiluminescent system (Boehringer Mannheim, Germany).
3. Results
3.1. Kinetics of Gene Expression of HBMEC Showing the Progression of Gene Profile Changes during C. neoformans Infection
Microbial invasion is a complex and dynamic process. We performed a time-course study to examine the gene expression profiles in HBMEC during C. neoformans infection. We selected C. neoformans strain B3501 for this study because the genomic sequence of this strain has been completed. In addition, we have used this strain for in vitro adhesion and transcytosis studies on HBMEC [11, 13]. It is an encapsulated strain with moderate adhesion activity to HBMEC, which was used as the in vitro model of BBB. A spectrum of 24 hours would allow us to evaluate its effect on HBMEC. Poly(A)+ RNAs derived from the incubated HBMEC at 0, 4, 8, 12, 16, 20, or 24 hours after B3501 incubation were subjected to the RNA extraction and the preparation of the biotinylated cRNA probe. The prepared probes at different time points were hybridized with biochip, individually. We used an Affymetric HU95A microarray chip harboring 12,559 human clones, facilitating efficient detection of changes in gene expression in the host cells. The expression levels were measured and analyzed by the GeneChip program. In a hierarchical clustering, the closet pair of expression values is grouped and the data points are organized in a phylogenetic tree in which the branch lengths represent the degree of similarity between the values. The mRNA profiles of HBMEC, assessed at 0, 4, 8, 12, 16, 20, and 24 hours after C. neoformans incubation, are shown in Figure 1.
These 12,559 gene patterns were classified into two major clusters (Clusters 0–12 hours and 16–24 hours) by using the GeneSpring software. Rapid changes in gene profiles at an early time point are followed by a gradual alteration until the 12-hour time point. Of interest, the gene patterns of the last three time points were similar, yet more distal related to the early gradual changes of gene profiles (0 to 12 hours). At these time points (16, 20, 24 hours), the gene profile seems to reach a plateau. The HBMEC is a homogeneous cell culture. The changes in the gene expression profiles are not due to the heterogeneity of cell cultures. The perturbation of gene expression profiles is most likely due to the presence of C. neoformans. Overall, the hierarchical clustering analysis of the mRNA levels reveals a sequential change in HBMEC infected with C. neoformans. The overall profile was altered more prominently at the initial stage of the pathogen-host interaction and subsequently persistent expression.
3.2. Comparison of Gene Expression Profiles from the Microarray and Protein Levels from Western Blot Analyses
To determine the validity of results obtained by the microarrayanalysis, some genes were subjected to Western analysis. These genes included -actin, Bcl-x, CD47, Bax, Bad, and Bcl-2 (see Figure 2). They were chosen for blot analysis because their protein levels were variable and the affinity of commercially available antibodies. For example, -actin mRNA and protein levels are maintained in a constant level at all time points, whereas CD47 mRNA and protein level increase to the 8 hour and then decline back to the normal levels. In general, the protein levels of these genes were expressed in varied degrees, and the protein expression profiles were comparable between microarray and protein analyses (see Figure 2). The results validated the competency of the microarray analysis for detection of changes in gene expression in HBMEC during C. neoformans infection.
3.3. Principal Component Analysis (PCA) of HBMEC Gene Profiles during C. neoformans Infection
In order to obtain a global grouping of gene profiles, we examined the PCA grouping of mRNA from HBMEC. PCA was a useful linear approach for obtaining a simplified visualization of entire datasets, without losing experimental information (variance). PCA allowed the dimension of complex data to be reduced and the most relevant features of a given dataset (transcriptome) to be highlighted. It was useful for our kinetic studies because it was possible to describe trends that were, otherwise, irretrievably by a direct examination of the entire dataset. The gene fluctuation could be classified into 7 major groups. The major group (PCA group 1) contained 4155 genes (33.08% of total analyzed genes) and the PCA group 2 contained 3129 genes (24.92% of total analyzed genes) (see Figure 3). Thus, these two groups represented more than 50% of HBMEC mRNAs. The gene profile of PCA group 1 showed a very slightly increase at 8 and 12 hours, and then a rapid decline. On the other hand, the PCA group 2 showed a gradual decline till 8 hours and then bounced back to near the original levels. These two profiles were nearly a mirror image. The results suggested that HBMEC altered its mRNA levels or adjusted its physiological status in response to the pathogen invasion.
3.4. Gene Expression Profiles at Different Time Points in HBMEC Analyzed by the Self-Organizing Maps (SOM)
The interpretation of system complexity is the most challenging task of biology in this century. The analysis of complexity in biological systems might start from a simplified representation of static gene networks and then move to an increasingly well-defined and integrated description of biological phenomena, bearing in mind that only dynamic networks will explain reality adequately. An example of a nonhierarchical clustering method is SOM. As SOM solves difficult high-dimensional and nonlinear problems. Using this algorithm, gene expression data are transformed into vectors or coordinates in an -dimensional space, where equals the number of variables or time points. Gene profiles of HBMEC postinfection at 7 time points (0, 4, 8, 12, 16, 20, 24 hours) were analyzed by SOM (see Figure 4). Upper bound (4.0), normal (1.0), and lower bound (0.0) were shown. The dynamic of gene expression during pathogen-host interaction can be clearly observed (see Figure 4). The use of SOM for grouping of different profiles greatly facilitates further analysis.
3.5. Persistently Upregulated and Down-Regulated Genes during C. neoformans Infection
Despites significant fluctuation of gene expression profiles in most HBMEC genes in response to C. neoformans infection, there were some genes that were upregulated or downregulated persistently during 24 hours period. One example of the upregulated genes was the cytochrome P450 gene which was continuously upregulated through the course of infection. A similar gene profile could be found and grouped, for example, genes that had a correlation at least 0.95 to the expression profile of cytochrome P450 were defined as upregulated genes (see Figure 5(a)). In the same manner, downregulated genes were grouped, in which genes that had a correlation at least 0.95 to the expression profile of troponin I type 3 gene TNNI3 (see Figure 5(b)).
3.6. Induced Fluctuation of Interferons and MHC Group Genes during C. neoformans Infection
We have performed the hierarchical and gene tree analyses to test whether the interferon genes and MHC-related genes were fluctuation during C. neoformans infection. The group of interferon-related genes (66 genes) was clustered based on their expression level (see Figure 6). Most genes were fluctuated during the course of infection, suggesting interferon played a role in the innate immune response to C. neoformans. Many infectious microbes induced expression of chemokines and adhesion molecules in human endothelial cells. Previous studies of the expression of IL-8, INF--inducible protein-10 (IP-10), MCP-1, and the leukocyte ligand ICAM-1 in primary HUVEC revealed that C. neoformans had the ability to interfere with inflammatory signaling in human endothelial cells, and suggested that C. neoformans might induce leukocyte activation and trafficking in the infected host (HUVEC) [5, 6]. Our results showed that IL-1 increased slightly, while MCP-1 did not change significantly. No changes in IP-10 and ICAM-1 expressions were observed in this chip. The TNF- profile was also increased, though quite late, during the infection. Similarly, the expression of a group of MHC genes including 29 of those in MHC II class was fluctuated (see Figure 7). Although some genes were listed but not expressed, our results suggested that endothelial cells contributed to the host immune response to C. neoformans infection. Taken together, HBMEC is not a professional immune cell; however, the fluctuation in the expression of interferons and MHC genes suggests that the innate immune systems are activated during the pathogen invasion.
4. Discussion
The current work demonstrates the use of cDNA microarray-based infectomic approach to characterize transcriptomes in HBMEC infected with the meningitic pathogen C. neoformans. Like most of meningitic pathogens, the penetration across the BBB that is constituted by BMEC is required for the pathogenesis of the CNS infection caused by C. neoformans [6, 14]. Our study represents the first investigation of the holistic transcriptional response of the in vitro BBB cell system in response to C. neoformans infection. A total of 12,559 human genes have been analyzed in this study, and distinct alterations in HBMEC gene expression have been observed at 4, 8, 12, 16, 20, and 24 hours of infection. Significant changes in the transcriptional infectomes were observed in HBMEC infected with C. neoformans. This approach was used to define the infectomic profiles of HBMEC infected with C. neoformans for a series of time points, thus, evaluating the dynamic changes in gene expression profiles and inflammatory factors in the host response to this fungal pathogen. The data were analyzed with several clustering analyses. In a hierarchical clustering analysis, the gene expression patterns at different time points displayed a progressive change of gene profiles. The first three incubated time points were clustered, and showed more drastic changes at initial engagement between C. neoformans and HBMEC (4 hours). The gene partners of the latter time points (16, 20, 24 hours) were clustered together, indicating the alternations reached to a plateau. Microarray analysis has been used for monitoring host gene expression profiles from other pathogen invasion studies, such as HIV-1. Many of the results were to compare the data pre- and post- infections. Our studies revealed that pathogen invasions are multifaceted and dynamics. A time point study is necessary to monitor the alterations. In a nonhierarchical analysis SOM, the two-dimensional space map displayed different expression profiles form 12,559 genes into 42 groups. One interesting finding defined by PCA is that one group of genes with graduate reduction in expression (~33% of total genes) accompanied with another group of genes showing gradually increased expression profile (~25% of total genes). The alternative changes in the two major groups (1 and 2) are shown as a mirror image (see Figure 3). The biological mechanism and significance of the expression profile changes in the pathogenesis of C. neoformans infection remain to be defined.
Most interestingly, the group of interferon-related genes (66 genes) was clustered based on their expression level. The expression of most genes fluctuated during the course of infection, suggesting that interferons play a role in the innate immune response to the pathogen. The expression of 29 MHC II class also fluctuated. Though some genes were listed but not expressed, the results suggested that endothelial cells contributed to the host immune response to C. neoformans infection. Many infectious microorganisms induced expression of chemokines and adhesion molecules in human endothelial cells. Studies of the gene expression in primary HUVEC revealed that C. neoformans had the ability to interfere with inflammatory signaling in human endothelial cells, and suggested that C. neoformans may later induce leukocyte activation and trafficking in the infected host (HUVEC) [15, 16]. Our results also showed that expression of interferon-related genes and MHC group genes are changed during C. neoformans infection. Collectively, the fluctuation in the expression of innate-related genes suggests that C. neoformans, like other microbial pathogens, is able to induce expression of chemokines and other innate genes.
The BBB has been considered an immunologically inactive organ as there are few antigen-presenting cells present in the CNS and the presence of the tight junction was thought to prevent the entry of immune cells from the peripheral circulations into the CNS [8]. However, increasing number of studies indicate that the endothelium of the BBB constitutes a dynamic and immunoactive interface between the blood and the CNS that can be modulated by endogenous factors such as bradykinin and cytokines, as well as exogenous factors including meningitic pathogens and their products [17]. Alterations in the BBB function are critical for the development of CNS infection including cryptococcal meningoencephalitis. It has been learnt about the immune response to cryptococcal infection from both in vitro and in vivo studies with the focus on immune cells [6, 18]. The immune response to C. neoformans infection was observed as one of the most interesting changes.
5. Conclusion
In summary, the current infectomic studies with a genome survey of C. neoformans-induced host response in HBMEC provide global information for the pathogenesis of the CNS infection caused by this fungus. Similar conclusions were reached when analyzing the microarray data with two complementary approaches, PCA and SOM. However, PCA works well in problem spaces that are linearly separable. SOM solves difficult high-dimensional and nonlinear problems. Most importantly, our study demonstrates for the first time that the BBB can actively participate in immune response to cryptococcal infection by regulating the expression of interferons, MHC and cytokines. It suggests that the BBB is not only an impermeable cellular barrier but also constitutes an immunoactive interface between the circulatory system and the CNS and that can be modulated by meningitic pathogens such as C. neoformans. Further insight into the role of the BBB in the pathogenesis of microbial meningitis and immunoregulation of the host defense against meningitic pathogens will gain a global view of the CNS infection and offer exciting prospects for advances in the prevention and therapy of this disease.
The authors would like to thank Dr. K. J. Kwon-Chung and Dr. Y. Chang for providing C. neoformans strains and intellectual inputs, and Dr. Kwang S. Kim for providing HBMEC. They also thank their colleagues for suggestions, help, and discussion. This work was supported in part by grants-in-aid from the NIH Grant no. RO1 NS047599 (A.Y.J.) and Grant no. RO1 AI40635 (S.H.H.).
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This quilt gave me fits. It was my first Log Cabin and I had never machine quilted before (I used to do it all by hand, but that takes too long). Lizze had just shown me how to do binding the "proper" way as well. This quilt was a mess waiting to happen.
I found the fabrics at my local quilt shop and I just loved them. It was there in the shop that I decided I should make a log cabin quilt. I got the blocks pieced just fine, but then came the quilting. My tension was off and I had big loops on the back. The best part is that I didn't know until I was ALL DONE since it looked fine on the front. As an inexperienced machine quilter I had no idea that I should stop and check once in awhile to make sure it was nice on both sides. And that weird noise my machine was making? Yes, I ignored it and kept on going.
I ended up ripping out all of the quilting I had done and went crying to Lizze's house. She let me use her Bernina to re-quilt it. Then came the binding… I did it wrong. I was so fed up with this quilt that I just folded it and put it away for months. Recently, I decided to try again. I took off the binding and did it correctly. Now that I have made several quilts using the proper binding technique, I felt much more confident. Now that it has been quilted twice, and bound twice… it is finally done.
No sooner do I get this quilt complete, I stumble upon a Log Cabin contest. Well, you know I had to enter. I'm certain that my quilt will not win, but I'm proud of it regardless. Voting starts tomorrow so I'll post a link if you're interested in voting for me 🙂
In the meantime, this quilt is listed for sale in our etsy shop (the link is on the right hand side of this page).


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Medical Marijuana
Canadian and European,autoflowering,feminised,indica,sativa,Cannabis Seeds
Monday, 13 August 2012
sensi seeds Bank - Jack Flash #5 - feminized
To create Jack Flash, Herer's sublime gene-pool was refined, reinforcing desired traits through back-crossing. Over multiple generations, our favourite Herer mother was infused with a precise blend of Super Skunk and Haze, undergoing rigorous selection and testing at every stage. By altering this program only slightly and adding a unique father plant to the mix in the final stage, we have been able to produce Jack Flash - a reliable, consistent, feminised variation on our spectacular Jack Flash. Jack Flash has the bulk and vigour of her Afghani-Skunk ancestors without sacrificing any of Herers sumptuous Haze high. In isolating this particular balance of traits, the four common phenotypes of Jack Herer are reduced to two recurring forms in Jack Flash, representing the contrasting sides of her heritage. The Indica form is compact enough for SOG, with rapid and highly distinctive bud development. Calyxes swell to outrageous sizes, piling atop each other to form resin-saturated ropes which build into multi-point crowns on terminal buds. The Sativa-dominant Jack Flash is a truly world-class example of cannabis. Growers with space for medium-to-large plants will be astounded by this phenotype - her flavour, yield and mind-body potency are virtually unparalleled. Significant height gain in flowering, paired with the ability to completely fill out with solid, running, Skunk-Haze bud means that best results are seen in when Jack Flashs Sativa form is allowed to finish at 1m or taller. While most members of the Sensi family rate Northern Lights#5 x Haze or Jack Herer as their all-time favourite ganja, Jack Flash is another serious contender for the title and is steadily gaining ground on the two world champion Sativas. Flowering: 55-75 days Yield: 125g
you can find it at
cannabis bud-beans.com
remebger you get 5% discount using code
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10 Things to Experience on the Trek to Everest Base Camp
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Categories: Everest BC Trek
There is something to admire around every turn of the 31 mile trail through the Khumbu Valley from Lukla to Everest Base Camp. Monasteries, cultivated fields, grazing yaks, and tiny villages all share the valley, sitting beneath the some of the world's tallest and most stunning mountains. A few of our guides sat down to compile a list of ten things to be sure and experience on the Everest Base Camp Trek:
10. View From Kala Patar: It's not widely known, but the view from the top of Kala Patar, a rocky outcropping above Gorak Shep, provides a sweeping panorama from Everest to Ama Dablam and are far better than those of Base Camp.
9. Lama Geshe: A renowned spiritual leader living in a small house in Pangboche, Lama Geshe never fails to greet visitors with a deep laugh and broad smile. As he chants prayers in his native Tibetan in a deep but soft voice, he provides a fascinating appreciation and insight into the Buddhist process of embarking on a journey, whether for an Everest Expedition or a trip to visit distant relatives.
8. Momos: Akin to Chinese dumplings, the Nepalese momo is a delicious treat after a long day on the trail.
7. Afternoon prayers at the Tengboche Monastery: After making the 1,300' ascent to the ridge top Monastery, find a seat along the edges of the Dokhang (the prayer hall), framed by a two story tall golden statue of Buddha, and listen to the deep chants of the resident monks fill the space.
6. Exploring Namche Bazaar: Perched on a hillside in the middle of the Khumbu, Namche Bazaar is the cultural and economic hub of this mountainous region. Yaks, monks, and Tibetan traders share the narrow streets with locals and trekkers alike, and a new discovery can be found around every corner, from tailors to bakeries to monasteries and museums.
5. The experience of trekking lodge-to-lodge: After a warm breakfast served in a cozy dining room, shoulder a light day pack with only the gear you need for the day, leaving your duffel outside your door, and start up the trail. Spend the day wandering through villages, fields, and valleys, until you reach your next night's teahouse where your bag awaits. The teahouses are simple but comfortable, with cozy community dining rooms to eat, relax, and socialize in and sleeping rooms just down the hall.
4. The Trail: While the excitement of trekking to Base Camp is reaching the foot of Everest at the end of the trail, the wonder comes from the entire experience of traveling the trail, where a simple centuries old footpath serves as both highway through the valley and the villages' Main Streets. Slow your pace now and again to look around and take in the shops, homes, and life that all happens right along the path!
3. Island Peak's summit ridge: Ringed by several of the world's greatest mountains (Nuptse, Lhotse, Everest, and Ama Dablam), the airy summit ridge of Island Peak provides all of the excitement of Himalayan climbing in a short extension to the spring trek.
2. Spending the night at Everest Base Camp: you've walked all that way, why turn around and leave again in a matter of hours? Spending the night in Base Camp is a peek into the full experience of embarking on an Everest Expedition and enjoying a cup of tea while watching the sun peak over the top of the Western Cwm is an unforgettable moment.
1. Stopping to soak it all in: The Khumbu is simply an amazing place. One of the best things is just being there: stopping to dig your heels into the ground and looking around. There is nowhere else in the world like it.
Ready to experience the Khumbu for yourself? Join RMI on a trek to Everest Base Camp!
Comments (5)
Please see our in depth blog about hiking to everest base camp. We have pictures, our budget, planning, and pictures/videos. It should be very helpful for you!
Posted by: Scott on
It is one of the best Everest Base Camp Trekking blog i have ever read. Really well written n knowledgeable thanks for sharing glad to read your blog.
Posted by: Everest Base Camp Trekking on
It is one of the best Everest Base Camp Trekking blog i have ever read. Really well written n knowledge able thanks for sharing glad to read your blog.
Posted by: Everest Base Camp Trekking on
I am very happy to see this blog. This is really meaningfully blog for Everest base camp trekking. I am also trek and tour operator from
Posted by: Prakash Lamsal on
Loved every minute! Highly recommend! Trip of a lifetime!
Posted by: Kelly on
More to Explore
Mt. Elbrus: Justman & Team Acclimate on the Mountain
July 23, 2014


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In honour of Breast Cancer Awareness Month, we will be launching a limited collection for the month of October! https://pink.snupped.com. Net profits from this campaign will be donated to the breast cancer foundation.
All artworks were contributed for a good cause and were solely done at the goodwill of our artists.
1 in 8 women will be diagnosed with breast cancer at some time during her life
Yes, that number is staggering. I was never aware of it till today. This isn't a death sentence, it is treatable, preventable and most importantly many survivors have lived to share their stories!
Early detection
Early detection is key in the treatment of breast cancer. There are steps you can take to detect breast cancer early when it is most treatable.
Women age 40 – 45 or older who are at average risk of breast cancer should have a mammogram once a year whilst women at high risk should have yearly mammograms along with an MRI starting at age 30.
Spread the message, save lives
Everyone has a part to play, even if you manage to get the message across to just one more person, that's fine! You may be saving someone's live.
For more information on breast cancer, do read more
Special thanks
I'd like to personally thank the artists and influencers who are putting in the effort to help spread the message. Together, we'll achieve more :)


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In September I started taking classes again after three years out of school. Currently I'm taking Historical Methods (online course) and Visual Literacy (evening class). I have four small paperback "textbooks" for my Historical Methods class that I've started reading in preparation for assignment one (more about that later), and so far there have been four Visual Literacy classes.
I'll talk a little more about my Historical Methods class in a future post, but I really wanted to share a list we were given in the second Visual Literacy class because it really resonated with me. While the list is simple, it's also insightful.
Dieter Rams Ten Principles of Good Design
1. Good design is Innovative.
2. Good design makes a product useful.
3. Good design is aesthetic.
4. Good design helps us to understand a product.
5. Good design is unobtrusive.
6. Good design is honest.
7. Good design is durable.
8. Good design is consequent to the last detail.
9. Good design is concerned with the environment.
10. Good design is as little as possible.
Visit one of my favourite architecture blogs to read a little bit more about Dieter Rams: http://www.lifeofanarchitect.com/who-is-dieter-rams/
While Wikipedia is not the most reliable source considering where the content originates from, you can still get a basic idea of the life of Dieter Rams here: http://en.wikipedia.org/wiki/Dieter_Rams
He seems like a fascinating person – definitely someone I can learn a lot from.
What do you think of Dieter Rams' Ten Principles of Good Design? Do you think he makes valid points?


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Tracker1's Blog
Something that tends to annoy me, is seeing a ton of string concatenation, such as below.
string mystring = "" +
ResourceHelper.GetString("somestr", SiteHelper.GetCurrentCulture()) +
The real issue is seeing the above code all on one line, and with the various html markup intersperced with various object variables. It can be very difficult to follow, especially when you see all this one one line, or not very well broken up. This leads me to the happy little method known as String.Format(), which takes a format string as its' first parameter, and a set of objects as parameters to be formatted into the string. Below is the above example rewritten to use String.Format().
string mystring = string.Format(
"{3}",
ResourceHelper.GetString("MyResourceKey", SiteHelper.GetCurrentCulture())
The above example is much more readable. The format string takes numbered parameters starting at 0 and can even take a format string such as for a date.
string formattedString = string.Format(
As you can see, using String.Format can make your formatted text much easier to follow and read than inline concatenation. I know the examples above apply to formatting text into html markup, and acknowledge that this isn't the *only* place where this happens, however it is one of the more common themes I see used over, and over again. If your string floats off to the right of the screen, and you can't easily follow the concatenation, then string.Format is probably a good idea. Especially combined with multi-line strings in C# (@""). Note, if you need a literal curly braces symbol ("{" or "}") simply double the character up ("{{" or "}}").
Tags: strings, c#, vb
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My name is Michael J. Ryan and I've been developing web based applications since the mid 90's.
I am an advanced Web UX developer with a near expert knowledge of JavaScript.


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It's been quite a while, but the best -and admittedly the only- Warhammer (pdf) e-zine made its comeback. At last. The 8th issue of Irresistible Force is available for download at irresistibleforce.net. And a worthy download of 20 Warhammer packed pages it is. Give it a read.
Related Tags: Warhammer, WHFB, Games Workshop, Miniatures, Game, Games, Ezine, pdf, E-zine, free, Magazine


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This is Bathtime Rules
Designer Janlynn
Date started - 01/02/12
Date finished - 11/20/13
I also did some catching up on
Mary's Sampler II
This is my progress as of 11/24/13.
I have just started the final section tonight so I hope to try and get some stitching done on it tomorrow.
I also HOPE to get back to Dinosaurs this week -
I have kinda missed it!
Thanks for taking a peek at my stitching
see ya next week with another faithful update!


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Create an Account - Increase your productivity, customize your experience, and engage in information you care about.
The Town Administrator is appointed by the Board of Selectmen to assist in the administration of the executive branch of town government. The Town Administrator is responsible for assembling the budget, keeping the Selectmen advised on unusual budgetary matters, preparing a long range fiscal plan, and performing special studies and analysis as the Selectmen may require. The Town Administrator has supervisory responsibility, maintains an inventory of town-owned property and a purchasing system, makes recommendations to improve town services and conducts studies and investigations pertaining town government.


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Nevertheless This can be reassuring, translations from animal to human scientific studies are constantly complicated, and the fact stays that the probable consequences of lasting supplementation are unknown. Bacopa Monnieri (leaf) Extract
The information supplied on This website is of the standard character only and isn't intended to exchange the recommendation within your physician or pharmacist. We do our best to keep up exact and entire information and facts that is certainly saved up-to-date, but we simply cannot (and do not) provide any guarantee of your precision of any information presented on this Internet site. For more information, make sure you see our disclosure policy.
There have been 89 subjects involved in this study, all of whom were being overweight women. They subjects were being break up into two teams: the initial team consumed garcinia cambogia thirty-60 minutes right before foods, when another team consumed a placebo at exactly the same instances. The study lasted twelve weeks: the topics' physique composition and fat had been calculated At the beginning with the research then just about every two months, whilst the topics' appetite variables and food items consumption ended up measured Initially, then at the time every month.
Usually keep in mind any person can post their very own customer testimonials and responses, we consider our greatest to filter out any purchaser opinions and comments which happen to be Evidently published by a company to market their own individual item or by a competitor to discredit a product. Nonetheless, if you see any doubtful shopper evaluations we might have skipped, make sure you Speak to us.
The reason caffeine is utilised so much is as it is actually a central anxious stimulant. It may perk people up and make them feel a lot more energetic and warn. Nonetheless, caffeine can become addictive rather easily, though the human body can speedily turn into tolerant to its outcomes.
Paradoxically, the detrimental results of alpha-adrenoreceptor blockade include decreased blood pressure level and drowsiness. A few of the far more severe side effects from extended exposure contain possible harm to the heart and also the anxious technique. Bauhinia Purpurea (leaf and pod) Extract
The powerhouse component you've been missing is building a comeback in HydroxyElite! 1,three Dimethylamylamine is back using a vengeance. Extracted from geranium, this compound acts being a sympathomimetic by unleashing the neurotransmitter norepinephrine, and that is unveiled during the flight-or- battle reaction. Norepinephrine allows activate lipolysis, the breakdown of Extra fat that will help you obtain Your whole body composition targets.
On the other hand, the addition of Garcinia Cambogia on the formula appears superfluous as this component lacks medical proof. Lastly, given that the opposite components of HydroxyElite's proprietary Mix are severely underneath-researched, it can be unclear whether they will offer weight-loss results.
Bacopa Monnieri (generally his comment is here known as Brahmi) is noted for its capability to strengthen cognitive function. This capacity is investigated some times in people and often times in animal scientific tests, For example in an effort to ascertain its usefulness to prevent cognitive deficit in schizophrenia. It has been proven to boost thyroid T4 stages, which implies it could indirectly lower the quantity of Extra fat saved in the body.
The most typical Unwanted effects of bacopa monnieri extracts seem to be nausea, increased intestinal motility and tummy upset. Furthermore, in a analyze wherever aged clients eaten bacopa monnieri, supplementation was identified to reduce the guts amount (this outcome correlated with minimized pressure ranges).
Rauwolscine appears to become the name for any material that may be similar to yohimbine, a chemical that is derived in the yohimbe plant, an evergreen tree that's found in Cameroon, Gabon and Zaire. Yohimbine is found in the bark with the yohimbe tree and is assumed to assist with sexual effectiveness by raising both of those nerve impulses and blood movement for the sexual organs.
Garcinia cambogia is believed to focus on Body fat burning by regulating cortisol manufacturing. Cortisol is often a steroid hormone that the adrenal gland releases in reaction to worry; Additionally it is assumed to boost blood sugar levels.
By the tip on the review it was mentioned that the Ladies who had been consuming garcinia cambogia had lost three.7kg on ordinary, while those that had consumed the placebo experienced shed two.4kg on normal. While The end result is encouraging, with one.3kg of weightloss separating the two groups, it's really worth bearing in your mind that this research did not contain any Adult males or any one not obese or overweight and it can be unsure if the similar outcomes will be made in Males or anyone obese or overweight.
A further examine checked out Check This Out the results of caffeine on metabolism by analysing caffeine's consequences on physique temperature and Electrical power expenditure. There were twelve male subjects involved with this analyze. They have been break up into two groups: the 1st group consumed 150ml decaffeinated coffee with 200mg caffeine, whilst the next consumed 150ml decaffeinated coffee by alone.
Top hydroxyelite Secrets
Nevertheless This can be reassuring, translations from animal to human scientific studies are constantly complicated, and the fact stays that the probable consequences of lasting supplementation are unknown. Bacopa Monnieri (leaf) Extract


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Site Spotlight: Now Play It
by findingDulcinea Staff
Now Play It, a site that offers video tutorials for learning to play the guitar, bass, drums, or piano parts to famous songs.
Can't quite get down that guitar intro for Roxanne? Allow Sting to teach it to you. Now Play It is a British music site with free video tutorials on famous tunes. The lessons are taught by the artists themselves and involve a 15-25 minute lesson, a recap, and a play-through. While the tutorials do cost a small fee to download, Now Play It offers a wide variety of tutorials on the guitar, bass, drums, and piano.


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I don't want to succumb to a defeatist mentality. This mentality is something I've actively tried to eradicate from my life, starting from finding at least one thing positive in even a negative incident to making an effort in noting a small positive trait in other people. Now, when it comes to having something to say from the ruminating part of my mind, for instance, to bring forth that thought experiment or idea that's been stewing in some recess of the brain, I just have not found the inner strength to fight defeat. I cannot overcome that idea that everything interesting and noteworthy has been said. I've finally understood the stark reality that writing down an idea has the lowest entry barrier and this statistically will work against someone seeking to break new ground in this area. Maybe I am late to the party. Maybe I am stating the most obvious. But I have just understood that creating something, something beyond words, is the true domain of creativity — at least a domain that hasn't had its final word.
01 Nov 2011 Leave a comment
in Blog, v1 Tags: creativity, goals, personal, thoughts, writing


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An analyst sounded a warning note on shares of biotechnology company Dendreon on Monday, which have more than tripled in value since March 30, but the stock continued to rise.
A Food and Drug Administration panel endorsed Provenge, a prostate cancer vaccine, on March 30, saying there was evidence the drug could be an effective cancer fighter. The agency usually follows panel recommendations in evaluating new drug applications.


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Phil Minissale represents the future for the acoustic finger-styling of the Piedmont. At just 24 years old, Phil is a seasoned performer, appearing at music venues, folk clubs, festivals and colleges from Maine to Georgia and recently abroad in Italy.
Phil's cross generational appeal has many folks watching very closely as he exposes his generation to the acoustic folk/blues sound. Combining contemporary songwriting with the traditional finger styling of the blues masters, his nostalgic sound is a warm reminder of the Greenwich Village acoustic scene of the early 60s while cultivating a new and younger audience.


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Discussion in 'Technical Analysis' started by paysense, Jun 1, 2008.
Will the Nasdaq once again find resistance at it's 200-day M.A.?
Investopedia: The "head-and-shoulders" pattern is believed to be one of the most reliable trend-reversal patterns.
Does this resemble the bearish pattern?
Market might stay in a range (S&P 1375-1425) for a while just to screw both bulls and bears.
...a few more tests? Then what?pS
Weak futures. . .now what?
Ok, well in the early going we get a 1.25% drop in the Dow and a 1.5% drop in both the Nasdaq and the Nasdaq 100.
A quick "lop-off" from gains made last week in lightened trade.
Since there has been no commentary on this "formation", I take it Investopedia has it nailed.
So if the support/"neckline" is broken or about another 1.25% - 1.5% fall. . .we head for a Bear Market?
I guess this site is mostly "scalpers" that are not interested in "swings" or general Market Direction. After all, T.A. and volume has no bearing on trades - just pure "price" action.
Either ET members are very much like the "crowd" (in denial) or I am looking at this wrong:
So people here would rather post about everything else - but this does seem like a possible staunch "reversal".
Yep, that was a bit of a chomp on last weeks gains:
Of course I'd be sorely remiss if I forgot the timely PPT!
I'd post some pretty pictures, but I'll just say - are we having fun yet?
Does anyone lend credence to this "reliable" bearish pattern?
What is wrong with this website? I am actually having a conversation with myself. It seems you have to act severely dysfunctional just to "incite" responses to a thread discussion.
Maybe I should've named the thread - "IT'S FOR SURE, GENTS, THE BEAR MARKET IS STARTING NOW!!!!"
I am just curious is the right shoulder does indeed seem to be headed sharply lower - i.e. going into a Bear Market very soon.
Please take a moment and post your views and experience.
Separate names with a comma.


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( Genesis 11:3 ; LXX., "asphalt;" RSV marg., "bitumen"). The vale of Siddim was full of slime pits ( 14:10 ). Jochebed daubed the "ark of bulrushes" with slime ( Exodus 2:3 ). (See PITCH .)
M.G. Easton M.A., D.D., Illustrated Bible Dictionary, Third Edition,
published by Thomas Nelson, 1897. Public Domain, copy freely.
[N] indicates this entry was also found in Nave's Topical Bible
[S] indicates this entry was also found in Smith's Bible Dictionary
Bibliography InformationEaston, Matthew George. "Entry for Slime". "Easton's Bible Dictionary".


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Carriers participating in modernized Connect America Fund programs must file deployment data with USAC's HUBB (High Cost Universal Broadband) portal showing where they are building out mass-market, high-speed internet service by precise location. This information includes latitude and longitude coordinates for every location where service is available, and USAC will eventually display this information on a public-facing map to show the impact of Connect America funding on broadband expansion throughout rural America.
The HUBB portal is accessible through USAC's E-File system. Once you are logged in, click on "HUBB" on the left-hand navigation panel. If you have permissions to file the Form 481, you will have the same permissions to file data through HUBB portal (e.g., user, officer, general contact). Users and general contacts can upload, validate, and save data. Officers can perform all of those functions as well as certify locations.
Carriers participating in the following funds have until March 1, 2018 to file latitude and longitude coordinates for all broadband locations newly deployed or upgraded during the following periods:
To help you prepare your data, we have created Geolocation Methods: A guide to successfully collecting broadband deployment data. This guide describes three methods for gathering geographic coordinates, with the pros and cons of each approach. You can use any method or combination of methods to gather the correct geographic coordinates for the locations you serve or could serve. Accurate data is the key to filing successfully with the HUBB portal.
USAC has been designing and developing the HUBB portal with your needs in mind.
We have conducted a comprehensive outreach campaign, meeting with carriers, consultants, engineers, and other stakeholders who will file data with the HUBB portal. We held multiple focus groups, conducted site visits, did extensive user prototype testing, participated in industry conferences and trade shows, held more than two dozen stakeholder meetings and briefings, and conducted a survey. The input we gathered is informing the design of this new portal.
Based on the feedback we received, we have included the following features in the HUBB portal:
We appreciate the time and effort so many of you have devoted to help ensure that this is a success. Thank you!
USAC is here to help you navigate these changes successfully. We will provide updates on this page, through the HUBB portal email distribution list, and through other ongoing communications with stakeholders. For regular updates, please sign up for the HUBB portal email distribution list, entitled "High Cost Detailed HUBB Updates," in the USAC's Subscription Center.
If you have any questions or concerns, contact us via email.


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I recently had a chance to review The Essential Step-By-Step Guide to Acupressure with Aromatherapy Treatments: Relief for 64 Common Health Conditions. This book provides a comprehensive look at acupressure and aromatherapy and how they can be used for a wide variety of conditions. The points are all easily explained, with pictures and with specific explanations on how to find the precise points on the body (since every body's body is slightly different). Then, many different conditions are listed, each one with the primary and secondary points that will help relieve the conditions, along with appropriate aromatherapy treatments.
While I don't believe that every single condition can be treated using alternative medicines like acupressure or aromatherapy, I do think that they can help with many different health conditions. They also can work in conjunction with traditional treatments (just be sure to let your health practitioners know about any alternative therapies you are also using).


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FULTON, NY – Wesley L. Lyons, 86, of Fulton, NY, died Saturday June 5, 2010, at Oswego Hospital.
He was born in Minetto and had been a resident of Minetto for most of his life.
Mr. Lyons was a World War II Veteran having served in the United States Marine Corps in the 8th Squadron as a Flying Sergeant.
Mr. Lyons was honored during his service with the following medals and awards: Â American Campaign Medal, Navy Occupation SVC Medal, WWII Victory Medal, Marine Corps Sharpshooter Rifle Badge, Honorable Service Lapel Button, and the Marine Corps Honorable Discharge Button.
He was past employed with Ball & Wilson, Oswego, NY.
Mr. Lyons was an avid Yankee fan.
He was predeceased by his wife of 59 years, Margaret (Forbes) Lyons, in 2005; and his son, John Lyons, in 2004.
He is survived by his children:  Wesley Lyons Jr. of Oswego, Margaret (Harrison) Wilde of Hannibal and Terry (Jennifer) Lyons of Panama, and Michele (Mark) Sherman of Fulton; two brothers:  Norman "Tom†Lyons of Minetto and Howard Lyons of Oswego; 13 grandchildren; 10 great-grandchildren; and several nieces and nephews.
Memorial services will be held 10 a.m. Tuesday at the Sugar Funeral Home, 224 W. Second St. S., Fulton with Rev. Richard Morisette officiating.
Burial with Military Honors will be conducted in Minetto Cemetery, Minetto.
There will be no calling hours.
The Sugar Funeral Home has care of the arrangements.


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Max Mortlock
- Santa Clarita US-CA
- [email protected]
- dynatekindustries.com/
- facebook.com/pages/Dynatek-Industries-Inc/175983285778337
- twitter.com/#!/DynatekInd
Our products are superior in every way offering guaranteed performance, higher tensile strength and hundred percent customer satisfaction- all for up to 25% less than our competitors. The highest quality parts, a proven manufacturing process, stringent quality controls and testing enable our products to beat the competitors on quality, performance, and price.
We carry a large inventory of standard and specialty shipping supplies.
We purchase all items in bulk so you don't have to. This allows our customers the opportunity to purchase supplies in smaller quantities at the lowest possible prices.
Every sale is important to us, which is why we treat everyone equally. Buy one item or a thousand items and we are just as happy! Remember that we buy in bulk so you don't have to.


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This blog is a part of our educational campaign on the occasion of National Payroll Week. In this blog, we will try to help you understand the basics of paycheck i.e. benefits, deductions, check, and direct deposit.
- What is a Paycheck?
- Difference between Gross Pay and Net Pay
- Deductions and Benefits
- Paycheck
- Direct Deposit
- Check


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Kaufmann Mercantile's grilling set is the perfect gift for dad this Father's Day. If your dad is a self-proclaimed grill master, give him a gift that he will actually use and enjoy. This barbecue accessory kit is equipped with a leather apron that features added pocket storage along with a steel and rosewood grilling set that includes a spatula, a knife, a two-tined fork and a sauce brush.
In addition to this barbecue kit's handy apron and grilling accessories, it is also sold with handmade Texas BBQ smoking bags. They come in three flavors including pecan, mesquite and honey and boast artisanal, burlap packaging.
Priced at a $321 rate, this chef gift set for dad will put a smile on his face this Father's Day.
Dad-Approved Grilling Sets
More Stats +/-
Dad-Celebrating Coffee Sets
Multifunctional Hipster Accessories
Artisanal Key Ring Accessories
Exclusive Male Grooming Clubs
Dad-Bod Perfecting Devices
Kaufmann Mercantile's Chef Gift Set for Dad Features BBQ Accessories
- By: Jana PijakJun 17, 2015
References: store.kaufmann-mercantile
References: store.kaufmann-mercantile


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The city of Key West is situated in Monroe County, Florida, USA. It's a sea port destination area of Florida. Key West has hotels and motels as well. The main business area of Key West is the Duval Street.
The main economic activities include Airlines, architecture, retail, counseling, banking, entertainment, financial advisory services, real estate, tourism, telephone services, education, photography and painting.


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The Honourable Minister who was represented at the event by the Permanent Secretary in the Ministry, Mrs Nkechi Ejele disclosed this while declaring open the workshop organized by the NCC for staff of the Ministry's newly created Entertainment and Creative Services Department held at the Conference Hall of the Ministry on September 11, 2014. He said he was "optimistic that the workshop will broaden the knowledge of staff of the Ministry which would help them face the daunting challenges ahead in the Entertainment Industry".
Also speaking at the event, the Director General, NCC, Mr Afam Ezekude described the workshop as a reaffirmation of the close linkage between entertainment, creativity and copyright while also stating that it was a demonstration of the unalloyed commitment of the Commission to promote inter-agency collaboration in the discharge of its mandate.
He thanked the minister for accepting the offer from the Commission to offer training for staff of the newly formed department which he said would open up new opportunities and promote mutually beneficial synergy to strengthen institutional capacities and enhance service delivery to the very important creative industries.
Speaking further, the DG said the training is designed to acquaint officers of the Department with the economic importance of the creative industries and how they interface with the Copyright System. The DG encouraged participants to take full advantage of the opportunity provided by the training.
The DG explained that he had directed the Nigerian Copyright Institute, NCI to identify other areas of collaboration with the Ministry and other Departments with a view to assisting them to deliver on their mandates. Lectures at the workshop were delivered by Mr John Asein, Director, NCI, Mr. Bitrus Dauda, Director, Planning, Research and Statistics, NCC, Mr. Austin Amodu, Director, Enforcement, NCC, and Mr. Mike Akpan, Deputy Director and Head, Regulatory Department, NCC.
The workshop which had the theme 'The Copyright System as a Catalyst for Growth in the Entertainment and Creative Sector' was attended by senior officers in the Entertainment and Creative Services Department of the Ministry and a team of Directors and staff of the NCC.
For: Director General


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The wait is over as Gazza announces album release
Windhoek "The wait is over, who's got my back?" reads a post by one the most celebrated local musicians, Lazarus "Gazza" Shiimi. Gazza last week announced the date for the release of his 11th album. Gazza who has put Namibian music on an international level over the past years by featuring and performing alongside big guns internationally, is set to release his much anticipated 25-track album on December 3, at the University of Namibia (Unam) stadium. He describes this as his biggest project so far featuring international renowned artists such as Davido, X-Trio, Revolution, Uhuru, Nyanda from Brick and Lace. Locally he has Lady May, Tequila, Suzy Eises, Maria Nepembe, Betty David, Lazarus Kaumbi, and many more. The album is titled Pumumu, and according to Gazza, the album has been produced with the penetration of the African market in mind. "To title that name it means the danger is penetrating the African market. This is not for Namibia any more but we are moving forward," says Gazza. He adds that the album is being distributed by the Universal Music Group, a deal that he signed end of last year. This means his songs will be distributed and made available to all purchasers and sellers of the mother company, making their music available in outlets such as Musica, Music Moods and all digital platforms affiliated with the Universal Music Group. On this album, one can look forward to songs which Gazza have worked with the Jamaican-born Nyanda Janelle Thorbourne of Brick and Lace fame, on his single Up up away. Other songs include his new single release, Get it on tonight, featuring saxophonist, Suzy Eises. "This is an album whereby I am expressing myself freely. I did not limit my creativity this time around as I did on the previous albums," says Gazza, adding that he has decided to do want he wants and to sing the way he wants. He says he cannot reveal the genres of the songs now as he will live that as surprises to his followers. "All I am asking is for people to buy original, to support local and not to copy the songs," pleads Gazza. Gazza who is ready to unveil his 11th album titled Pumumu on December 3.
New Era Reporter
2016-11-25 12:21:47 2 years ago


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the former Yugoslav Republic of Macedonia
- FYR Macedonia: Cold Wave - Jan 2017
- FYR Macedonia: Flash Floods - Aug 2016
- FYR Macedonia: Flash Floods and Mudslides - Aug 2015
- South-Eastern Europe: Floods - Feb 2015
- FYR Macedonia: Cold Wave - Dec 2014
- FYR Macedonia: Floods - Feb 2013
- Europe/Northern Africa: Cold Wave - Jan 2012
- Influenza A (H1N1) Pandemic - Apr 2009
- The former Yugoslav Republic of Macedonia: Forest Fires - Jul 2007
- Central/Eastern Europe: Heat Wave - Jul 2007
Most read reports
- Wildfire management focus of OSCE organized workshop for South-East Europe
- Factsheet on the former Yugoslav Republic of Macedonia - Last updated 07/02/2018
- The former Yugoslav Republic of Macedonia: Location Map (2013)
- 6 February 2015: Southern Europe -Severe weather
- the former Yugoslav Republic of Macedonia - Floods - ECHO Daily Map | 08/08/2016
Strategic Assessment
The global terrorist threat continued to evolve rapidly in 2015, becoming increasingly decentralized and diffuse. Terrorist groups continued to exploit an absence of credible and effective state institutions, where avenues for free and peaceful expression of opinion were blocked, justice systems lacked credibility, and where security force abuses and government corruption went unchecked.
I. Introduction
1. A significant increase in the numbers of persons of concern to UNHCR brought new challenges in 2006. While there was a 14 per cent increase in refugee numbers from the previous year, the Office's involvement, together with other humanitarian partners, in protecting and assisting internally displaced persons (IDPs) under the inter-agency cluster approach, resulted in a doubling of IDP figures. Thanks to better data capturing, many more stateless people have been identified, also swelling numbers.


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The current MLS listings of homes for sale in Rim Rock are displayed below. If you see a home that interests you or want to set up a NO Sales Pressure appointment to see homes in Rim Rock, call Jim at 512.913.1557.
♦ Belterra ♦ Friendship Ranch ♦ Highpointe ♦ Hills of Texas ♦ La Ventana (Driftwood) ♦ Ledge Stone ♦ Reunion Ranch ♦ Rim Rock ♦ Sawyer Ranch ♦ More Southwest Austin Neighborhoods w/interactive map ♦
Click link to see featured S-SW Austin Neighborhoods (East of Brodie Ln.), with an interactive Google map.
Home sale activity over the past in Rim Rock subdivision are summarized in the table that follows.
# of Homes Sold
Median Sales Price
Avg. Sales Price
Sales Price Range
Avg. Days on Mkt.
Age of Homes Sold
Sq. Ft. of Homes Sold
Rim Rock real estate market data from the Austin Board of Realtors, 1/1/2011-12/31/2017.
Rim Rock is designed to provide homeowners with the peaceful experience of living out in the Central Texas Hill Country. It is located just 19 miles from Downtown Austin via FM-1826. From Rim Rock, you can get to all things Austin and Central Texas with little trouble (depending on traffic, of course!). Rim Rock is a nice alternative for families who want to live in the country, but on larger lots than are available in subdivisions like Belterra and Highpointe (neighborhood links at the top of the page) or Meridian.
In Rim Rock, there are three key options for potential homeowners: (1) Purchase a lot in the neighborhood and choose a custom builder. (2) Purchase an inventory or to-be-built home from one of the major builders in Rim Rock. (3) Buy a home that is on the resale market.
All Rim Rock homes are built on lots a minimum of 1 acre in size. The largest lot available in the original section in April 2009 was just over 3 acres. Some lots have Hill Country views and back up to green areas.
As can be seen in the table above, previously-sold homes in Rim Rock come in a wide range of sizes: from 2,566 sq. ft. to over 5,000 sq. ft. Prices also varied widely in 2016: from $395k to $889k. The average sales price was $560k.
In addition to several custom home builders, there are several major area home builders are currently building homes in Rim Rock. These are Scott Felder, Century Communities, Vintage Estate Homes, Coventry Homes, and Dream Finder Homes.
These Rim Rock builders offer 1- and 2-story options with many standard features and plenty of other elevation and upgrade options for buyers who want to personalize their homes. New home sizes (base plans) vary widely.
Residents of Rim Rock live just a short drive from Southwest Austin and all it offers. Shopping venues include The Shops at Arbor Trails (Costco), Escarpment Village (Circle C Ranch, HEB grocery store), and the Sam's Club & WalMart Super Center developments at Mopac and US-290/71.
The community is also near the Grey Rock and Plum Creek Golf Courses, the Lady Bird Johnson Wildflower Center, the Veloway, Hamilton Pool and other outdoor attractions.
Tax rates in Rim Rock, like those in many outlying residential developments, are higher than in Austin, for example. Why? Though there are no city taxes, the water service is provided through the Greenhawe WCID (water control and improvement district). Taxes from this WCID brought the total tax rate to near $2.94/$100 in property value in 2015. Other tax entities include the school district and Hays County.
All of the homes in Rimrock have septic systems. Electricity is provided by Pedernales Electric.
Rim Rock students learn the 3 R's and more at three schools of the Dripping Springs Independent School District. Since school attandance zones are redrawn occasionally, parents should verify which schools are assigned to the Rim Rock neighborhood with the Dripping Springs ISD.
"We love because He first loved us." (1 John 4:19, NKJV)
Jim Gilbert, e-Pro®, REALTOR®
Austin, Texas Real Estate Agent
Licensed by the Texas Real Estate Commission (#0536692) to sell Homes & Real Estate in Texas
4501 Spicewood Springs Rd., #1029, Austin, TX 78759 ♦ (512) 342-8744
Texas Real Estate Commission Information about Brokerage Services
Terms of Use and Privacy Policy


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The Guns n' Roses CD was not the only artist we have had to wait for 17 years for new music.
Grace Jones new CD is "Hurricane" and it friggin' great. I have been a fan for years and while some of her past music can be a tad frivolous this new CD is a sonic blast. Always a great visual artist she has 9 new songs to put forth a whole package. Check out her new video "Corporate Criminal" on YouTube.com It is incredible. To me it sounds very industrial, like she had been listening to Nine Inch Nails. Never the best singer (I admit that half of her appeal is presentation) her voice has been layered to great effect on this CD. Former Prince protege's Wendy & Lisa also contributed a song. The CD is great.
The bad news is that it is, so far, only being issued in Europe but is available as an import. For the life of me I never understand how music is marketed. It is obvious that a lot of money and effort went into the project, why not make it available for those that want to experience it?
Worth your while.
Wednesday, November 26, 2008
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HARK! Are those sleigh-bells I hear?
I know it seems a tad early to be bringing up the holiday season now. I mean, we finally have some summer weather here in the Pacific Northwest, but after August 6th (this coming Saturday) Hearth & Larder will be taking two months off the whole market thing. However, I'll be back in the Fall beginning October 1st. And right around the corner are the Holidays and you know what THAT means...HOLIDAY BAKING!!! So, from November 26th through December 31st I will be making holiday items: Sourdough Stollen Rolls (pictured above), Lebkuchen (German Christmas Cookies), Snowballs and a few TBA items.
The Tri-Cities Baker's Community Market
Evergreen Cultural Centre, 1205 Pinetree Way, Coquitlam BC V3B 7Y3
Evergreen Cultural Centre, 1205 Pinetree Way, Coquitlam BC V3B 7Y3
OCTOBER 1st & 8th
NOVEMBER 12th & 26th
NOVEMBER 12th & 26th
Moberly Arts & Cultural Centre, 7646 Prince Albert Street, Vancouver, BC V5X 3Z4
OCTOBER 15th
NOVEMBER 5th
DECEMBER 3rd & 10th
(a special Christmas market!)
(a special Christmas market!)
And now for some product news.
|Pomelo-Lime Marmalade
I always like to experiment with jams, jellies and marmalades, especially citrus flavors. Earlier this year a made some lime marmalade. A lot of lime marmalade. Entirely TOO much lime marmalade. And it's pretty sharp tasting and I don't know many people that would really like it. So I thought "how can I keep the lime flavor but reduce the face contorting factor?" Heading down to my favorite produce store I picked up some pomelos. For those of you unfamiliar with pomelos they are giant citrus fruit similar to a grapefruit but very mild and the texture is a little crunchy. I know, weird. But adding it to the limes sort of cut the overly harsh taste and with a little vanilla bean added to the mix made for a highly flavorful and unique marmalade flavor. Thus Pomelo-Lime Marmalade was born! It will make an appearance at this Saturday's market and subsequent markets while supplies last.


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Every person who has been born again is equipped to evangelize the lost. So why is it that some of us are hesitant to engage unbelievers with the gospel? Ladies, let's continue to be encouraged by what God's Word says about evangelism.
Click here to listen to this episode of Equipping Eve.
Click here for a list of resources used on this episode and the previous episode of Equipping Eve.
Additional Resources
Equipping Eve: Equipped to Evangelize, Part 1
Equipping Eve: Simeon's Psalm
Equipping Eve: Counted Worthy to Suffer


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|MPolish Metallics stamped over Bear Pawlish Sacrifice
|But there's not even a minimum spend on Monday 5/25!!
For my mani today, I decided I wanted to do something a little different. I started with a base of Qtica Natural Nail Growth Stimulator and a coat of OPI Nail Envy. My base polish is Bear Pawlish Sacrifice. Sacrifice is a stunning silver polish with a strong linear holo and holo micro glitter scattered throughout. It is a very smooth polish and I had no trouble controlling it when polishing. It is greatly pigmented so you could get away with one coat and it would be fully opaque, but I used two coats for this mani.
|One coat of Bear Pawlish Sacrifice for full coverage
|Bear Pawlish Sacrifice, two coats and no topcoat
|Bear Pawlish Sacrifice
|Two coats of Bear Pawlish Sacrifice topped with Bear Pawlish Oso Quick
|Oso Quick quick dry topcoat over Bear Pawlish Sacrifice
|Macro of Bear Pawlish Sacrifice - look at that holo microglitter!
|Pet'la Calaveras stamping plate
|MPolish Baroque and Elizabethan stamped over Bear Pawlish Sacrifice
|Hand Pose inspired by SloppySwatches
|Macro of MPolish Metallics stamped over Bear Pawlish Sacrifice with lead lighting
|Sugar Skull image from Pet'la Calaveras stamping plate
And I couldn't let you go without a sun shot of Sacrifice! This polish is gorgeous in sunlight!!
|Holo effect of Bear Pawlish Sacrifice in direct sunlight
So don't forget, automatic FREE SHIPPING to US and Canada on Monday 5/25/15 for all MPolish orders, no minimum spend necessary and no codes to enter! She is also completely restocking the store, so if you haven't already gotten your hands on the glowing Spring collection, or her fantastic summer POMP collection, now's your chance!


text: Paintings Used In Signs Welcome, Guest. Please login or register. 1 Hour 1 Day 1 Week 1 Month Forever M. Night Fans Home Films Home Help Search Calendar Login Register MNightFans.com » Films » Signs » Paintings Used In Signs « previous next » Print Pages: [1] Go Down Author Topic: Paintings Used In Signs (Read 4091 times) QUESTIONER Praying with Anger Offline 2 Paintings Used In Signs « on: June 19, 2006, 07:11:53 PM » Does anyone know who painted the paintings above the fireplace and bed? Logged QUESTIONER Praying with Anger Offline 2 Paintings Used In Signs « Reply #1 on: June 20, 2006, 05:57:45 PM » "Stone City, Iowa" - by Grant Wood Logged Dal Wide Awake Offline 24 Paintings Used In Signs « Reply #2 on: August 05, 2006, 02:14:44 PM » its a nice painting, reminds me of the film. Logged Print Pages: [1] Go Up « previous next » MNightFans.com » Films » Signs » Paintings Used In Signs SMF 2.0.14 | SMF © 2017, Simple Machines Films: Praying with Anger | Wide Awake | The Sixth Sense | Unbreakable | Signs | The Village | Lady in the Water | The Happening | The Last Airbender | After Earth This site is maintained and updated by fans of M. Night Shyamalan, and is in no way affiliated with M. Night Shyamalan or Blinding Edge Pictures. We in no way claim the artwork displayed to be our own. Copyrights and trademarks for the books, films, articles, and other promotional materials are held by their respective owners and their use is allowed under the fair use clause of the Copyright Law. Design and original photography however are Copyright © 2004 - 2015 MNightFans.com. Related Fan Sites: Chronicles of Narnia Movie News | Christopher Nolan | Last Airbender | Legendarium | Matrix Trilogy | SweetPaul | The Terminator | Walden Media XHTML RSS WAP2 Page created in 0.065 seconds with 20 queries.