text:
Key scientific publications
Hou, Y. et al. (2015) Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing. GigaScience 4:37
Compared to other whole genome amplification methods, REPLI-g Single Cell Kits are the best in class for variant calling and are the optimal solution if SNVs and CNVs are of similar importance, as in tumor heterogeneity or cell evolution research.
Korfhage, C. et al. (2015) Parallel WGA and WTA for Comparative Genome and Transcriptome NGS Analysis Using Tiny Cell Numbers. Curr Protoc Mol Biol. 2015 Jul 1
Korfhage, C. et al. (2015) Whole-Transcriptome Amplification of Single Cells for Next-Generation Sequencing. Curr Protoc Mol Biol. 2015 Jul 1
Korfhage, C. et al. (2013) Whole-genome amplification of single-cell genomes for next-generation sequencing. Curr Protoc Mol Biol. 2013 Oct 11
Zhang, C.-Z. et al. (2015) Calibrating genomic and allelic coverage bias in single-cell sequencing, Nat. Comm. 6, 6822
Cancer, neuroscience or stem cell research
Approaches suitable for solid tumors have proven limited for circulating tumor samples due to the low levels of input DNA. Furthermore, whole genome amplification methods are not always compatible with blood collection tube preservatives. In this study, the REPLI-g Single Cell Kit was successfully used for whole genome amplification when combined with a multiplex targeted resequencing approach. The results could stand as proof of concept for real-time monitoring of patient tumors using noninvasive liquid biopsies.
Zhang, C.-Z. et al. (2015) Chromothripsis from DNA damage in micronuclei. Nature, published online 27 May 2015
Chromothripsis is a catastrophic mutational event that results in massive rearrangements of one or more chromosomes. It helps to drive cancer development through oncogene amplification, tumor suppressor loss and DNA damage response impairment. This milestone paper describes a study on individual cells selected based on live cell imaging and subjected to single cell whole genome sequencing. The approach (called "LookSeq") allowed identification of the first molecular mechanism of chromothripsis. Whole genome amplification (WGA) with highly uniform genome coverage is a prerequisite for successful single cell sequencing. The superior performance of QIAGEN's REPLI-g Single Cell Kit in WGA was critical for this research.
Yao, X. et al. (2014) Tumor cells are dislodged into the pulmonary vein during lobectomy., J Thorac Cardiovasc Surg.148(6)
This study focused on the contribution of intraoperative tumor shedding to tumor recurrence, using single cell genetic approaches to distinguish between normal and malignant epithelial cells. Whole genome amplification was performed using the REPLI-g Single Cell Kit, followed by amplicon sequencing using the Lung Cancer Panel within the GeneRead DNAseq Targeted Exon Enrichment Panels for target enrichment. After library preparation, barcoded pools were sequenced with paired-end 150 reads using the Illumina MiSeq. Analysis of copy number variation, nested PCR-based mutation analysis of single cells and targeted sequencing were used to identify tumor cells and determine if they were shed into circulation during surgery. The paper shows that single cell genetic approaches together with patient-matched normal and tumor tissues can accurately quantify the number of shed tumor cells.
Wang, Y.. et al. (2014) Clonal evolution in breast cancer revealed by single nucleus genome sequencing, Nature 512
Sequencing studies of breast tumor cohorts have identified many prevalent mutations, but insight into the genomic diversity within tumors is still difficult to attain in bulk tissues. This paper presents a high-coverage method for whole genome and exome single cell sequencing with applications in clonal and mutational evolution (copy number diversity and subclonal mutation analysis). Multiple displacement amplification (MDA) was performed on individual FACS-sorted nuclei using QIAGEN's REPLI-g technology. The sequence libraries were first sequenced at low coverage depth (1x). Libraries passing quality control were selected for full genome or exome sequencing. The method shows excellent performance, with uniform coverage, low allelic dropout rates and low false positive error rates for point mutations.
Hou, Y. et al. (2012) Single-cell exome sequencing and monoclonal evolution of a JAK2-negative myeloproliferative neoplasm. Cell 148, 873
Jeong, J.H. (2015) Cholinergic neurons in the dorsomedial hypothalamus regulate mouse brown adipose tissue metabolism, Mol Metab 4
RT-PCR analysis revealed the co-expression of muscarinic acetylcholine receptor (M2 mAChR) and tryptophan hydroxylase 2 (Tph2) in single cells collected from mouse brain slices. When combined with other results, these data support the existence of a novel neurochemically specific circuit that modulates thermogenesis and gene expression in interscapular brown adipose tissue.
Lee, D.K., Jeong, J.H., Chun, S.-K., Chua, S. and Jo, Y.-H. (2015) Interplay between glucose and leptin signaling determines the strength of GABAergic synapses at POMC neurons. Nat. Comm. 6, Article: 6618.
This study investigates the interplay between glucose and leptin signaling in glutamatergic proopiomelanocortin (POMC) neurons. Single cell qRT-PCR was used to measure the abundance of transcripts involved in leptin signaling to help build a model that integrates both glucose and leptin signaling pathways in POMC neurons.
Lee, D. et al. (2015) Apelin-13 Enhances Arcuate POMC Neuron Activity via Inhibiting M-Current. PLoS ONE 10(3): e0119457.doi:10.1371/journal.pone.0119457
Aneuploidy and genome profiling in blastomere research
Wells, D. et al. (2015) Clinical utilisation of a rapid low-pass whole genome sequencing technique for the diagnosis of aneuploidy in human embryos prior to implantation. J Med Genet. 2014 Aug;51(8)
Thornhill, A.R. et al. (2015) Karyomapping—a comprehensive means of simultaneous monogenic and cytogenetic PGD: comparison with standard approaches in real time for Marfan syndrome, Journal of Assisted Reproduction and Genetics, Volume 32
Xu, J. et al. (2015) Embryo Genome Profiling by Single-Cell Sequencing for Preimplantation Genetic Diagnosis in a β-Thalassemia Family Clinical Chemistry 61:4
Wang, L. et al. (2014) Detection of Chromosomal Aneuploidy in Human Pre-implantation Embryos by Next Generation Sequencing, Biology of Reproduction March 19,2014
Choi, Y.H. et al. (2015) Accuracy of preimplantation genetic diagnosis in equine in vivo-recovered and in vitro-produced blastocysts. Reprod Fertil Dev. 2015 Mar 17. doi: 10.1071/RD14419.
The researchers worked with 10–30 cell biopsies and conducted whole genome amplification (WGA) followed by multiplexed PCR assays designed to detect a variety of heritable equine diseases. With such small amounts of input material, WGA comprises a crucial step in the workflow, since it's exceedingly difficult to design specific, accurate multiplexed assays with such little DNA. Choosing the right WGA method is crucial – REPLI-g MDA technology outperformed another MDA-based solution in Choi et al.'s experiments: REPLI-g results were accurate in 98.2% of total loci analyzed.
Microbiology, infectious disease research, metagenomics
Eloe-Fadrosh, E.A. et al. (2016) Global metagenomic survey reveals a new bacterial candidate phylum in geothermal springs. Nat. Commun. 7, Article number: 10476 doi:10.1038/ncomms10476
By analyzing metagenomic data from IMG/M combined with single-cell genomics from samples collected from geothermal springs, Eloe-Fadrosh et al. were able to discover and describe a novel bacterial candidate phylum ('Candidatus Kryptonia'). Starting with a collection of 31955 long assembled contigs (≥ 100kbp) from 4290 metagenomic data sets (IMG/M), 744 contigs with non-redundant SSU rRNA (small-subunti ribosomal RNA) were further selected, aligned and placed on a reference phylogenetic tree. This led finally to the identification of a distinct lineage and near-complete recovery of four distinct genomes. In addition to metagenomics data mining, single cell sampling from four geothermal springs was performed. Single cells from the samples were isolated using FACS sorting, followed by WGA with REPLI-g Single Cell Kit, PCR screening for genomes from single cells matching 'Ca. Kryptonia' SSU rRNA sequences and sequenced on Illumina MiSeq. 18 single cell genomes with an average genome completeness of 67% were recovered. The high-quality draft genomes from metagenomic data analysis and single-cell genomics enabled further analyses in bacteria-virus interaction and the detection of a novel fusion between two different CRISPR-Cas types, which represents the first description of a type I-B/type III-A CRISPR-Cas fusion.
Chen, Yi-ran et al. (2016) Novel species and expanded distribution of ellipsoidal multicellular magnetotactic prokaryotes. Environmental Microbiology Reports 8(2), 218-226
The research in this study is on a specific morphotype of multicellular magnetotactic prokaryotes (MMPs), which are groups of 10 to 100 cells of diverse magnetotactic bacteria. Phylogenetic analysis of these MMPs was performed by isolating the specific morphotype (ellipsoidal MMPs) by micromanipulation, followed by WGA using the REPLI-g Single Cell Kit and 16S rRNA gene based sequence analysis. The phylogenetic analysis revealed similarities and differences of species composition of MMPs from various sampling sites, indicating as well a new candidate species.
Nakamura, K. et al. (2016) Culture-independent method for identification of microbial enzyme-encoding genes by activity-based single-cell sequencing using a water-in-oil microdroplet platform, Sci Rep. 2016 Feb 26;6:22259. doi: 10.1038/srep22259
The authors describe an activity-based single-cell sequencing approach enabling the visual evaluation of microbial cells during screening and offering a new targeted approach of specific isolation of microbial cells. Environmental microbes were encapsulated in water-oil microdroplets (containing a fluorogenic substrate for a target enzyme) This enabled the screening for microdroplets that contain cells active for the specific enzyme. The selected microbial cells were recovered and subjected to whole genome amplification using the REPLI-g Single Cell Kit. Successfully amplified single cell genomes were further analyzed by whole genome sequencing.
Mansor, M. et al. (2015) Metabolic diversity and ecological niches of Achromatium populations revealed with single-cell genomic sequencing, Frontiers in Microbiology, Vol. 6
Single cell sequencing is used in this study to uncover the anabolic and metabolic potential of the bacterial genus Achromatium and the genetic heterogeneity of Achromatium populations. Achromatium cells were enriched from sediment samples of a spring, flow-sorted and finally hand-picked using a micromanipulator. Whole genome amplification of the single cells was done with the REPLI-g Single Cell Kit. The WGA-DNA samples containing 16SrRNA gene sequences affiliated with the genus Achromatium were further analysed by whole genome sequencing. Draft genomes with 80% completeness were achieved. The draft genomes provided a basis of further analyses of the metabolic versatility of Achromatium providing new insights for further discussions on the mechanisms of intracellular calcification.
Nair, S. et al. (2014) Single-cell genomics for dissection of complex malaria infections, Genome Res. 2014. 24:1028-1038
The study describes optimization of an accurate single-cell genomics approach for malaria parasites that is applicable to both cultivable and noncultivable malaria species to reveal within-host variation. To determine malarial genotypes, the researchers developed a single-cell approach in which they isolated single infected red blood cells, used whole genome amplification and then genotyped and sequenced the parasites using next-generation sequencing.
Zoll, J. et al. (2015) Direct multiplexed whole genome sequencing of respiratory tract samples reveals full viral genomic information, Journal of Clinical Virology 66 (2015) 6–11
A proof-of-concept for virome determination by NGS. Virome determination is enabled by parallel preamplification of viral DNA and RNA with the REPLI-g Cell WGA & WTA Kit.
Bavelaar, H.H. (2015) Whole genome sequencing of fecal samples as a tool for the diagnosis and genetic characterization of norovirus. J. Clin. Virol. 72, 122.
A new proof of principle study by Bavelaar et al. shows that NGS is equally applicable in the detection and characterization of other norovirus genotypes. The authors performed direct multiplexed whole genome sequencing on fecal samples from patients with gastroenteritis. The study included eight norovirus positive fecal samples (samples 1–8) that had been previously characterized using the Noronet typing tool and fecal samples from two patients known to have gastroenteritis caused by norovirus but with unknown genotypes (samples 9 and 10). Sufficient amounts of RNA were isolated from all samples to perform whole transcriptome sequencing for the detection of RNA viruses using the REPLI-g WTA Single Cell Kit.
Lai Y., et al. (2015) Metagenomic Human Repiratory Air in a Hospital Environment. PLoS ONE 10(10): e0139044. doi:10.1371/journal.pone.0139044


text:
|2012-09-06, 12:05
|Link #23
Senior Member
Join Date: Dec 2005
And this is EXACTLY the reason why I was hoping that Apollo would be the star of the next sequel. AJ's plot was still heavily centered around Phoenix even though he was technically supposed to be taking a backseat for the new generation. I mean, don't get me wrong, while I'm glad to see Nick again (and yes, I'm also loving that new suit), it still would have been cooler if there were more focus on the next generation of lawyers, IMO. Heck, if even Danganronpa's sequel can focus on an (almost) entirely new cast, then why not AA.....?
Well, putting aside my rant for now, I am glad to learn that AA5 has been confirmed for localization. Now all we need is Layton vs. AA (as well as the long-overdue release of Investigations 2) and it'll be all gravy.
|2012-09-06, 13:14
|Link #25
Senior Member
Join Date: Dec 2005
Actually, speaking of Danganronpa, it would be all kinds of badass if that series and AA had a crossover. Everyone would look like cardboard cut-outs, the court battles would have crazy light gun-style objections and rhythm minigames, we'd get to formulate the whole picture of the crime through a comic, etc..... man, would that be sweet. Plus, I'd love to see the exchanges between characters like Naegi and Edgeworth, Nick and Togami, Larry and Hagakure, etc.... but the chances of such an epic crossover becoming a reality are pretty low for the moment, sadly.....
|2012-09-20, 00:40
|Link #26
Okuyasu the Bird
Join Date: Sep 2009
Location: Alberta, Canada
Age: 27
Subbed Tokyo Game Show trailer showcasing some actual gameplay which seems to utilize characters' emotional spikes to spot where to pressure their testimonies:
Phoenix's new partner's Japanese name is Kidzuki Konoe. (It'll likely be changed in the localization.) She's apparently an apprentice of Phoenix's law firm and aspires to become an attorney, similar to Apollo.
And there's a mysterious silhouette at the end of the video of another character. I wonder who it could be?
Last edited by SilverSyko; 2012-09-20 at 00:52.
|2012-09-20, 00:51
|Link #27
Though I'll miss the old sprites and such, the models and reactions look pretty good to me~
The silhoutte at the end doesn't exactly reminds me of anyone... though it COULD be a new villian. Thouuuuugh... appearances do change, so can't wait to see who it is~
|2013-03-13, 06:20
|Link #31
ダメ人 - 人間失格
Join Date: Jul 2011
Location: Germany
Age: 32
Eye bandage, bandaged hands/arms and a jacket? Bad@ss. Seems like he got into serious trouble/conflicts. The jacket seems somewhat military-alike, due to the one star on the shoulder region of it (but could also be just decoration )
|2013-03-17, 14:42
|Link #33
Okuyasu the Bird
Join Date: Sep 2009
Location: Alberta, Canada
Age: 27
Seems like the gimmick this time around is called "Heart Scope", and it's not something Phoenix can do himself, but rather it's an ability his disciple Kokone can do.
While it's only shown to be used in trials at the moment, it could possibly be used in the investigation portions of the game as well.
Personally I'd like to see Phoenix's psyche-lock abilities return again too and get some depth on those black ones that appeared in Apollo Justice. Those were so mysterious I'd like to know more about them.
Last edited by SilverSyko; 2013-03-17 at 20:55.
|2013-03-17, 17:53
|Link #34
Join Date: Jul 2007
Location: England.
Age: 28
I'm pretty sure a black Psyche-Lock is just meant to represent a secret so well-guarded that Phoenix would never be able to bring it forth in his lifetime. Hell, even when you cornered the person who had them in the courtroom, they still never admitted to anything.
Otherwise yeah, I agree, the Psyche-Locks did add a lot to the investigation phase and I was pretty bummed out they were taken out and not replaced with anything for Ace Attorney 4. I'd also be okay with the Psyche-Locks being left out if you can use the Heart Scope in the investigation phase instead.
In other news, Apollo is awesome. I'm guessing he'll be a witness in the first case and a supporting character in the future cases.
|2013-03-17, 22:58
|Link #38
Join Date: Jan 2006
Location: Dollars
Seems like he experienced major 'accident' or something that made him like that.
|2013-03-18, 06:01
|Link #39
Senior Member
|2013-05-13, 14:00
|Link #40
Senior Member
Join Date: Dec 2005
Huh, kinda surprised nobody posted this yet:
So we now know it's coming out in the West..... as a download-only title. Well, better that way than not being released at all, I say. But I guess this means the same fate awaits Layton vs. Ace Attorney and Investigations 2 if either title manages to see the light of day outside Japan....
|Thread Tools

subset: commoncrawl


text:
firstname.lastname@example.com 9AM - 6PM
Executive Desks | Modern - [Slim Chair]
For CEO´s and Top Level Management, a limited circle of office furniture where product perfection is a core value. Therefore all production processes: from purchase and selection of the best natural wood veneers and cow hides to pre-assembly and distribution. We care a lot about our finishes and all our wood veneers are worked by hand and the end product is assigned a unique serial number, which makes tracking easy and guarantees a personalized after sales service.
No products available


text:
Skip to comments.Good Ol Boy, Inc.
Posted on 12/31/2013 6:45:28 AM PST by KeyLargo
December 31, 2013 4:00 AM Good Ol Boy, Inc. Reality shows about gold miners, ax men, and ice-road truckers are a far cry from the Kardashians. By Victor Davis Hanson
The hysteria over Duck Dynasty reminds us that cable TV is currently inundated with working-class, white-guy reality shows. Top-drawing, relatively low-cost realities showcase gold miners, oil drillers, hunters, locomotive drivers, off-the-grid backwoods eccentrics, fishermen, crabmen, truck drivers, ax men, moonshiners, or the new generation of Beverly Hillbillies. The list of the particular subspecies of the muscular classes is endless.
These shows share a few common themes. They do not take place in an office, where most Americans work. They are not Kardashian psychodramas about plastic surgery gone bad, or a Gucci purse that underwhelmed the latte bunch in Brentwood. The men appear a bit beefier, perhaps stronger, but not necessarily more fit than your average American suburbanite. A big gut can add gravitas to the moonshiners biceps in a way impossible to achieve at the gym.
These men dont quite shave each day, and close-up shots suggest that none use tooth whiteners. There are no Tony Robbinses on the chain-saw crew. Their speech usually is Southern-inflected, or at least rural-sounding. They are almost exclusively white, but do not seem to especially worry that they are. Whatever their actual income, the players clearly think of themselves as solidly middle to lower middle class. They work with the sorts of machines, many of them dangerous huge trucks on ice, four-foot-long chain saws, earth-moving equipment, industrial arc welders that most Americans do not even know how to start up.
(Excerpt) Read more at nationalreview.com ...
-guess the low spot of all was when Dakota Fred dug himself so deep in the melting gravel bank that he couldn't even swing the backhoe---
Mr. Mercat watches some of them because he loves nothing better than taking a chain saw and cutting up stuff. He also likes plumbing work but I don't think that there's a reality show about plumbers. Maybe there should be. I don't like the bleeping. He has not watch DD. I don't know why.
All episodes of AMERICAN PLUMBER here:
Uploaded on May 2, 2007
Eddie Cirigliano is a hot-blooded Italian-American plumber with a short fuse, a huge smile, and a heart of gold. As a plumber-he's old school. He actually comes when you call, and he knows what he's doing- he's been at it since he was 14. His customers love him- usually.
"But there is yet a third and perhaps more likely hypothesis (none of these are mutually exclusive), well beside the notions that viewers are either amused by the slapstick or satisfied they at least have not yet sunk so low. The national hysteria over Duck Dynastys successful war against GLAAD and the profit-minded A&E execs might suggest that the white working classes also are therapeutic for millions of Americans. Suburbanites and urban dwellers usually dont know much about nature; their jobs are not predicated on muscular strength. A fir, a pine, a spruce they are all just trees. Who cares where north is, or whether the wind blows westward? Todays youth dont know the difference between a rip and a cross-cut saw.
Yet Americanus suburbanus also has a vague sense that for all the talk about the Dow Jones, Apple, Twitter, and Facebook economy, someone somewhere else still must be doing something that accounts for lumber at Lowes, gas at the corner Stop-N-Go, granite counters in the kitchen, and arugula at Whole Foods."
Hanson need not look outside of the offices of NR to find Americanus Pussus.
The attraction to these shows is the glimpse into the lives of REAL MEN.
No metro-sexuals on these shows.
They are SELF-SUFFICIENT.
They do real work.
And you don't find some government agency trying to make work conditions easier for women!!!!!!
LOL Thanks. I'll share that with Mr. M
September 13, 2012
defining ideas
Profile in Classics: Victor Davis Hanson
by Emily Esfahani Smith
We in the West forget the wisdom of the ancient past at our own peril.
Same here. I especially enjoy "Ice Road Truckers" and its spinoff, the show where they drive the world's most dangerous roads.
I will say, though, that I can't stand "Call of the Wildman." I think I lose I.Q. points just by watching the commercials for that show.
Like I-40 thru downtown Knoxville, TN?
I like Alex Deborgorski the best of the Ice Road Truckers.
" The first inside look at industry legend and fan favorite Alex Debogorski, a devout Christian, father of 11 children, and the worlds best known truck driver."
LOL, never driven it, but I take your word for it.
Maybe the show should check it out.
Yep, he comes across as a good guy. That belly laugh of his is something else.
Reality show about plumbers.
What would they call it "Plumbers Crack Me Up"?
They are scripted because if they showed what actually goes on in these types of jobs the viewers would probably nasty their drawers and the government types would be on their way to the job to shut down the work because pretty every job of this sort can not be done by the rule book. I is impossible to not break regulations dreamed up by people with no experience in the real world. Paper pushers, bean counters, and other assorted useless folk whose goal in life is to put out of business anyone who would attempt to pull himself up by his bootstraps and make a financial success of his life.
I hear all the time the lament, bring back American jobs, like we, inherited them and therefore own them, got news for ya, we didn't and we don't. They were ours as long as we earned them by the sweat of our brow, and we could do them cheaper and with higher quality. We are no longer a nation of builders or creators, but a nation of whiners.
Rough men and women built this country and until we become like them we will be losers on the financial battleground.
Where come from anybody with more then 4 teeth is considered a showoff and is trying to be 'above his raisin', but when the shtf you would be wise to have him at your side.
Disclaimer: Opinions posted on Free Republic are those of the individual posters and do not necessarily represent the opinion of Free Republic or its management. All materials posted herein are protected by copyright law and the exemption for fair use of copyrighted works.


text:
Globalization, technological change, and growing economic interconnections are challenging traditional strategy approaches. Yet strategy is more necessary than ever. The frequency and speed with which leadership positions change are increasing—and the performance gap between winners and losers is widening. To beat those trends, strategists must understand, compete across, and adapt to, a much broader range of strategic environments, each requiring distinct approaches and capabilities.
At the heart of all business strategy is the trade-off between exploration versus exploitation. Learn how to navigate the course of your business's journey.Our latest thinking on strategy
The Center for Sensing and Mining the Future draws on BCG's global trend database and helps companies better understand developments that will shape their future.Explore our solutions
A targeted strategy helps a bank capture Millennial customers at twice the rate of its conventional rivals.Read more about our impact
Senior Partner & Managing Director, Global Leader Strategy PracticeEmail Go to experts


text:
From the Roots Up, Developing Trees for Sustainable Biofuel
By Marcia Goodrich | Published
New tree varieties with wonder roots could one day supercharge the biofuel industry.
Scientists at Michigan Technological University aim to develop poplar trees with roots that enable them to thrive in dry, infertile soils. Their work is funded by a three-year, $900,000 grant from the joint USDA and Department of Energy's Plant Feedstock Genomics for Bioenergy Research Program.
"Biofuel crops will be grown on marginal lands to avoid competition with food production and curb greenhouse gas emissions," says Victor Busov, leading project principal investigator. "These plants will have to be pretty robust, and we're focusing on the roots."
The poplar is more than a top-quality source of biofuel. It was the first tree to have its entire genome sequenced. Thus, researchers can search among the poplar's 45,000 genes to find those that regulate its root system—and which genetic variations might result in roots that seek out water and nitrogen more efficiently from the soil. The more efficient the roots, the bigger the trees—and the more biomass to turn into biofuel.
The project has two complementary parts, says Busov, an associate professor in the School of Forest Resources and Environmental Science. In the first, poplars will be grown in dry, nitrogen-poor conditions, and researchers will track how the trees' genome responds. Then they will map which genetic networks control those responses and modify the key genetic "hubs" that govern those networks in an effort to grow trees with roots better suited to low-nitrogen, dry soils.
In the second experiment, they will generate random mutations in poplars and grow them under similar dry, infertile conditions. Should any of the plants respond well to drought and low nitrogen, the scientists will track which genetic changes are responsible.
Once the scientists identify which genetic modifications produce better roots, they plan to use a variety of approaches, including genetic modifications and traditional breeding techniques, to develop the ideal poplar varieties for biofuel production on marginal lands.
Developing trees that grow big and healthy on poor, droughty soils could help boost biofuel production and reduce dependence on petroleum. Most importantly, it could bring the biofuel industry another step closer to environmental sustainability.
Biofuels may seem like a clean, green alternative to petroleum. But intensive agriculture, for food or for biofuel, can generate a whole array of environmental problems, says Busov.
Typically, 30 percent to 80 percent of nitrogen fertilizers applied to croplands escape to contaminate water, contribute to greenhouse gas emissions, and even increase the incidence of disease vectors like some types of mosquitoes. Irrigation can lead to waterlogging and salinization and divert water from residential and industrial use.
Plus, the energy required to irrigate cropland and to manufacture and apply fertilizer pours even more greenhouse gas into the atmosphere. "Using nitrogen and water more efficiently to produce biomass is simply more sustainable," says Busov. "If we start on the wrong foot, we are setting ourselves up for disaster in the long run. Sustainable biofuel production is inseparable from efficient nitrogen and water use."
Co-principal investigators from Michigan Tech on the project are Assistant Professor Hairong Wei and Postdoctoral Researcher Yordan Yordanov, both of the School of Forest Resources and Environmental Science.
Michigan Technological University is a public research university, home to more than 7,000 students from 54 countries. Founded in 1885, the University offers more than 120 undergraduate and graduate degree programs in science and technology, engineering, forestry, business and economics, health professions, humanities, mathematics, and social sciences. Our campus in Michigan's Upper Peninsula overlooks the Keweenaw Waterway and is just a few miles from Lake Superior.


text:
Discussion area for Off Highway Vehicle (OHV) legislation, litigation, organizational issues, etc.
Use this control to limit the display of threads to those newer than the specified time frame.
Allows you to choose the data by which the thread list will be sorted.
Order threads in...
Note: when sorting by date, 'descending order' will show the newest results first.
Forum Rules


text:
|Episode Description
|Saturday, Sep 15, 2012 - 7:00 AM
"The Crusader"
This episode delves into the increasingly grey area between right and wrong as television heroes confront internal demons while seeking their own forms of justice. Interviews include Alan Alda ("M*A*S*H"); Steven Bochco and David Milch ("NYPD Blues"); Chris Carter and Gillian Anderson ("The X-Files"); and David Shore and Hugh Laurie ("House"). G
|More on WORLD (PT)
|No upcoming shows in database


text:
Atmospheric Sciences & Global Change
Research Highlights
December 2012
The Middle Child of Tropical Clouds
Mid-level tropical clouds form differently during active and break monsoon periods
Hovering between the large anvils from storm-forming cumulonimbus clouds at the top, and low boundary layer clouds, mid-level clouds are more difficult to characterize. Finding the origin and characteristics of these clouds in the middle will help provide a better picture of the tropical circulation. Shown in the foreground are meteorological instruments and radiometers. Photo taken at the Tropical Western Pacific site of the ARM Climate Research Facility, located in Darwin, Australia. Enlarge Image. Photo courtesy of ARM.
Results: Scientists at Pacific Northwest National Laboratory employed a method to understand how and when mid-level clouds, hovering between their conspicuous "siblings," form in the tropics. Low and high clouds get most of the attention-because they are easier to detect. The researchers found that the timing and temperature of these thin clouds signal whether they form when the monsoon storms originate over the ocean, or primarily over land. The results show a more complete picture of how clouds influence the global climate.
"In the active monsoon phase, when storms come from the ocean, these mid-layer clouds are more often found after ice precipitation melts and cools the layer, causing more water vapor to condense into a cloud," said Dr. Laura Riihimaki, lead author and atmospheric scientist at PNNL. "In the break monsoon phase, the clouds come primarily from over land. A greater percentage of those mid-level clouds come from direct injection of cloud particles into the layer."
Why It Matters: Thin, mid-level clouds can be quite extensive in the tropics. They reflect a large amount of sunlight back into space and trap heat in the atmosphere. Sandwiched between cloud layers, mid-level clouds have a big effect on temperature and moisture, yet are difficult for scientists to simulate in climate models. Like the overlooked middle child, mid-level clouds are often underestimated. This underestimation makes it difficult to simulate the entire picture of tropical air circulation, including the fuel for tropical storms: heat and moisture. A better view of mid-level clouds and how they influence both is essential to understanding how tropical circulation affects the global climate.
Methods: Satellite and surface views of clouds cannot see mid-level clouds because higher or lower clouds block them. PNNL scientists used a technique called vertically pointing, or "soda-straw" view, to observe cloud formation using lidar and radar together to get a complete picture of how these clouds in the middle occur. This study provides a unique climatology based on four years of observations at the U.S. Department of Energy's Atmospheric Radiation Measurement (ARM) Climate Research Facility's site in Darwin, Australia. The research team combined this technique with data from radiosondes launched on weather balloons, which gather atmospheric measurements, and a scanning precipitation radar, which observes precipitation.
Their results show that thin mid-level clouds more frequently follow stratiform precipitation during the active monsoon rather than the break monsoon period. Cloud layers are more likely to coincide with warmer, more stable layers during the break period. How these clouds form influences the moisture and temperature at mid-levels, which is important to the region's convection profile.
What's Next? The scientists want to characterize how water vapor at the mid-levels of the troposphere changes before and after occurrences of these clouds. Next, they will compare the cloud occurrence results in different monsoon periods with models to improve moisture-cloud process simulation. Also, future work can compare different methods of determining whether clouds are ice or liquid particles to improve cloud calculations in radiative heating rates and fluxes.
Sponsor: The U.S. Department of Energy's Office of Science Biological and Environmental Research Atmospheric System Research program.
User Facility: Data collected at the ARM Climate Research Facility, a U.S. DOE user facility, was used in this study.
Research Team: Laura D. Riihimaki, Sally A. McFarlane, and Jennifer M. Comstock of PNNL.
Research Area: Climate & Earth Systems Science
Reference: Riihimaki LD, SA McFarlane, and JM Comstock. 2012. "Climatology and Formation of Tropical Midlevel Clouds at the Darwin ARM Site." Journal of Climate 25(19):6835-6850. DOI:10.1175/JCLI-D-11-00599.1.


text:
Party Menu: A Spring Picnic in Pink and Green
What better way to celebrate the green shoots and pink blooms of spring than with an outdoor meal of the season's most iconic produce? We're thinking bright green asparagus, thin slices of pink radish, tender peas and tart rhubarb — get ready to grab a blanket and head outside!
A sandwich of hard-boiled eggs, fat asparagus spears and pickled onions on a crusty baguette travels well, along with a thermos of bright green fresh pea soup to stave off any early spring chills. A mayonnaise-free red potato salad with crunchy radishes, smoked olive oil and ponzu is a surprising twist on the usual, while a non-alcoholic sparkling rosemary limeade is an herb-infused alternative to lemonade. Finish the meal with a lightly sweet muffin studded with rhubarb and strawberry and take a moment to lie back and enjoy the day. Spring is here!
Get the recipes:
• Boiled Egg, Seared Asparagus & Pickled Onion Sandwich
• Fresh Pea Soup
• Red Potato Salad with Scallions & Radishes
• Sparkling Rosemary Limeade
• Strawberry Rhubarb Muffins
What would you bring on a spring picnic?
Related: 6 Vegetarian Dinner Menus for Spring
(Images: See linked posts for image credits)


text:
N Logo – An excellent logo template highly suitable for Internet, networking and communications businesses. Also good logo template highly suitable for media and entertainment.
It can be used by professionals in the photography and design, art products, branded as consumer products, banks, insurance company, night clubs, mobile app, any stores, programing software etc.
Unique logo template in 2 logo styles in horizontal and vertical versions in different color variations.
Included : AI, EPS_10, CDR.
File is easy to editing. All colors and text can be modified.
Font used : Aero Matics – http://www.dafont.com/aero-matics.font
View also
Fast Search
If you buy and like it, please rate it! Thank you


text:
Events for 7 Mar 2017
Events Search and Views Navigation
All Day
Fear and Love at the Design Museum
Fear and Love presents installations by some of the most innovative designers and architects working today, including Andrés Jaque's Intimate Strangers.
Forms of Action – Contestador
The Centre for Contemporary Arts in Glasgow (CCA) will host the exhibition Forms of Action, in which the Spanish artist and researcher Asunción Molinos is showing her work 'Contestador'.
Course: Women in Spanish Cinema
Eight weeks course focused on the roles and representations of women in Spanish Cinema, analysing the empowerment proccess from the 40s until now.
Dora García: These Books Were Alive; They Spoke To Me!
These books were alive; they spoke to me! is an exhibition of printed matter and performance works by artist Dora García
Antoni Tàpies: Revulsion and Desire
An exhibition of Antoni Tàpies (1923–2012) is been displayed at Timothy Taylor. It presents in London some works that have never been shown outside of Spain before.
Darwin's Tortoise at Cervantes Theatre
After the success of Blood Wedding last year, the Cervantes Theatre presents Darwin's Tortoise, its second production this year.
Pablo Genovés: Tides
Spanish artist Pablo Genovés' project Tides is on display in St Paul's Cathedral until 30 August as part of the international collaboration Just Water
100 Years of Roca | 100 Years of Design explores the dynamic connection between marketing communications and the evolution of Roca, from its origins in 1917 as a family workshop in Barcelona, to becoming a global bathroom company.


text:
Yesterday the Supreme Court released its first four opinions in cases argued this term, the latest first-opinion release in recent history. The only one that interests me – and it's not Justice Sotomayor's maiden effort – is the civil forfeiture case, Alvarez v. Smith.
Civil forfeiture, the practice in which the police seize cars, money and other kinds of property that they say has some connection to crime, can raise various legal and policy issues — from property rights to due process. The question in Alvarez was the basic one of whether people seeking to get their property back are entitled to a prompt hearing before a judge.
I blogged about the case here, and Cato adjunct scholar Ilya Somin wrote about it here. Cato's also filed a brief in the case supporting the individuals whose property was seized.
Unfortunately, because all underlying disputes had been resolved by the time of oral argument – cars had been returned and the individuals have either forfeited their cash or accepted the state's return of some of it – the Court determined the case to be moot. It thus vacated the lower court's opinion and remanded with instructions for that court to dismiss the case.
And that's a shame. While the dispute does seem to be moot with respect to the particular petitioners, this is obviously a situation "capable of repetition" but "evading review" – along the lines of that little-known case of Roe v. Wade. That is, just like the case of a pregnant woman is moot within nine months, disputes over civil forfeiture get resolved one way or the other long before the slow turn of litigation reaches the Supreme Court. By avoiding the merits of this case, the Court guarantees that the important constitutional questions presented by this case remain perpetually unresolved.
What is more, by vacating the Seventh Circuit's opinion – an extraordinary remedy – the Court deprives Illinoisans of a well-reasoned and just ruling that could be used as precedent in future cases. It also – and this is no small matter – wastes the time, effort, and resources of the parties and their attorneys, taxpayers (who obviously paid for the petitioners' legal work here, as well as that of the judiciary), and, of course, amici (including Cato).
Justice Stevens was correct in his partial dissent: if the Court disagrees with the argument I made in the preceding paragraph, it should have applied the general rule against vacating judgments that have become moot because the parties settled. The proper disposition here would have been to DIG the case – dismiss the writ of certiorari as improvidently granted (which allows the lower court ruling to remain on the books undisturbed).


text:
Angiotensin converting enzyme (ACE) inhibition for arterial hypertension lowers the risk of recurrent chronic subdural hematoma (CSH) by an anti-angiogenic mechanism
Inhibition des Angiotensin Converting Enzyms (ACE) zur Blutdruckeinstellung senkt das Risiko einer Rezidiventwicklung beim chronisch subduralen Hämatom (CSH) über einen antiangiogenen Effekt
Suche in Medline nach
|4. Mai 2005
Chronic subdural hematoma is characterized by an outer membrane with a rich an pathological vascularization. There is growing evidence that angiogenic mechanisms are involved in the formation of leaky vessels leading to hematoma enlargement and recurrence. We tested the hypothesis that the antiangiogenic effect of ACE-inhibition for arterial hypertension also reduces the risk of recurrence in CSH.
We analysed retrospectively the data of 392 patients with CSH treated in our department between 1995 and 2002. Only patients who underwent a standard procedure with one or two burrholes and postoperative drainage were evaluated. Patients with coagulopathies and independent neurological disorders were excluded from the study. Patient records were screened for age, gender, preoperative and postoperative Markwalder score, arterial hypertension, medication with ACE-inhibitors, and recurrence of CSH. Recurrence was defined as an increasing lesion on the operated side in cross sectional imaging and concomittant neurological deterioration within 6 months postoperatively. Additionally, in 40 consecutive patients who met the inclusion criteria the concentration of vascular endothelial growth factor (VEGF) was analysed within hematoma samples and corresponding venous blood.
Overall 279 patients met the inclusion criteria. Demographic data of group A (ACE-inhibitors) and group B (non ACE-inhibitors) did not differ significantly. Five of 46 (11%) patients in group A suffered recurrence from CSH whereas 62 of 233 (27%) in group B needed a second or third operation (p<0.05). A negative correlation was found between the yearly rates of medication with ACE-inhibitors and recurrence (r=-0.7; p<0.05). The concentration of VEGF in the hematoma was significantly lower in group A (7314 pg/ml versus 14494 pg/ml, p=0.01).
ACE-inhibition for arterial hypertension lowers the risk of recurrence in patients operated for CSH by an anti-angiogenic mechanism.


text:
Faculty Directory
Carol Winkler, MSN, RN, PNP, PMHNP
Faculty Fellow
- Email:
- email@example.com
- Phone:
- 716.286.8148
Professor Winkler is a new member of the School of Nursing. She is an alumnus of Niagara University; she is also a graduate of University of Buffalo and University of Rochester. She has many years of experience in pediatric nursing. Professor Winkler is presently practicing as a psychiatric nurse practitioner working with children and adolescents.


text:
Nicole Polizzi doesn't want her kids to know the name 'Snooki'
December 22, 2017
Share This Video
Nicole Polizzi doesn't want her kids to know the name 'Snooki'
Nicole "Snooki" Polizzi dishes on life after "Jersey Shore." The reality star doesn't want her son, Lorenzo, to know her famous nickname just yet. In fact, she and her co-star Jenni "JWoww" Farley want their kids to steer clear of their reality-show antics. "Don't black out and hide in a bush. Don't do those stupid things," Polizzi told PageSix.com's Maggie Coughlan over a holiday crafting table.
Miss Universe 2018's funniest moments
Miss USA was "basic." Miss Nepal nearly wiped out. And Miss Great Britain belted out a lousy Kelly Clarkson. The 67th Miss Universe pageant was full of funny fails — and a few historic firsts, too. Spain's Angela Ponce competed as the first transgender contestant in Miss Universe history. Meanwhile, Catriona Gray, from the Philippines, snatched the crown. Relive the best moments from the FOX telecast. This is "Who Onesie'd The Night?"
Céline Dion's style evolution: From '80s excess to totally epic
Celine Dion has always been a powerhouse performer, but her style has gone through some rough patches. Watch how her fashion has evolved over the years.
Tom Cruise explains how to turn off 'soap opera' motion smoothing on TVs
Tom Cruise released a PSA about an issue that's very important to him: motion smoothing. Modern HDTVs come with this feature automatically turned on, creating what is sometimes called the "soap opera effect." The unfortunate result is that many people don't get to watch movies the way the filmmakers intended.
Taylor Swift's style went from country chic to glamorous gowns
When Taylor Swift exploded onto the scene as a country sweetheart in 2006, curly hair and cowboy boots were her style signatures. But over the last decade, her music has evolved along with her look. Taylor's not afraid to take on new looks, from daring dark lips to sophisticated Met Gala gowns. Her fashion aesthetic may always be in flux, but one thing's for sure: She never goes out of style.
This could be basic cable's craziest sex scene
Can sex bring a coma patient back to life? According to TNT's "Claws," it's certainly worth a shot. In one of the most shocking sex scenes to hit basic cable, beautician Jennifer — played by Brooklyn-based actress Jenn Lyon — climbs atop her comatose husband and miraculously revives him. Watch the play-by-play with "Steamy and Streamy" co-hosts Lea Palmieri and Brian Faas. And for more steaminess, check out Decider After Dark.
'The Good Place' star Jameela Jamil feuds with Cardi B on Twitter over celebrity diets
If you're a celebrity advertising diet products online, Jameela Jamil is coming for you. "I've already lost 35 pounds," the British beauty joked in her toot-filled parody video on Twitter and Instagram. Jamil, who stars in NBC's "The Good Place," called out Cardi B, Perez Hilton and more.
'RHONJ' star would rather her husband be gay than with another woman
The cast of "The Real Housewives of New Jersey" sat down with Page Six to play a game of "Husband Hall Pass" where they shared whom they would allow their husbands to date if something happened to them. While some would let their men get it on with Ramona Singer or Dorit Kemsley, other ladies said they would rather have their man be gay.
The headline-making love story of Gabrielle and Dwyane
Gabrielle Union may be nine years older than Dwyane Wade, but that hasn't stopped them from being one of Hollywood's hottest couples. This installment of "From Hookup to Hitched" goes inside their relationship, from co-hosting a Super Bowl party in 2007 to their storybook Miami wedding in 2014.
A thirsty Carson Daly had the cringiest moment on the 'People's Choice Awards'
The inaugural "People's Choice Awards" on E! had its share of cringey moments. From Nicki Minaj's doubt-inducing double wins to a three-way kiss from "The Voice" crew, the night was full of winners and losers. But the question on everyone's mind is: "Who Onesie'd the Night?" It's no surprise, then, that the Kardashian-Jenners stole the show. Matriarch Kris Jenner mysteriously wore sunglasses throughout the entire two-hour live event — even while "Keeping Up with the Kardashians" won Reality Show of 2018.


text:
This dramatic hip hop instrumental stirs up hopeful, triumphant, victorious, determined, gritty, and excited emotions. The track features banging drums, massive synths, and an epic electric guitar lead that slowly builds and builds all the way through the track. This track would be perfect for dramatic and modern video presentations for sports videos, commercials, and various other promos that would suit a massive hip hop club track.
Both a full mix and a version without the lead guitars are included to better suit the use under a voiceover. You'll find high rez wav and 320kbps mp3 files included in the main files.
1. The Rise of a Legend Full 2:12 2. The Rise of a Legend – no lead 2:12
This music is part of my Hip Hop Club Pack. Get this and two other great hip hop instrumentals at a huge discount!.
If you liked this track check out another great hip hop track I'm sure you'll love called Hip Hop Club Banger


text:
|Head Coach
|Bogata, Colombia
|Alma Mater:
|East Stroudsburg
|Graduating Year:
In 2013, Oklahoma posted a 24-8 campaign that ended in the NCAA Second Round. Sallie McLaurin repeated as an All-American with an AVCA All-America Second Team selection.
Restrepo has turned around a program that won a combined 27 matches in the four years prior to his arrival. In 10 years at Oklahoma, Restrepo's record stands at 209-136 (.606). He has guided the program to eight NCAA Championship appearances, including two Sweet 16 appearances in 2006 and 2010.
During his tenure, 10 Oklahoma student-athletes have earned All-America honors from the American Volleyball Coaches Association and the organization has recognized 10 Sooners with all-region accolades. The Big 12 Conference has selected 16 OU players as all-conference selections and eight rookies have earned a spot on the conference's all-freshman team.
Seven OU players have garnered various Big 12 Conference "of the Year" honors and 61 have earned Academic All-Big 12 awards. A total of 13 Sooners have been awarded with the College Sports Information Directors' Academic All-District distinction. Suzy Boulavsky became the program's first Academic All-American in 2010 after being named to the CoSIDA third team. Boulavsky earned first team honors in 2011.
The 2012 senior class became the first to reach the postseason in each of its four seasons, only to be matched by the 2013 class. The 2013 season ended in the NCAA Second Round with a 24-8 overall record and 11-5 mark in the Big 12, good enough for third. McLaurin claimed her third AVCA All-America honor and earned CoSIDA Academic All-American as well as Big 12 Scholar Athlete of the Year.
The 2011 team finished with a 21-12 overall record and 8-8 mark in the Big 12 Conference. Oklahoma advanced to its third consecutive NCAA Championship that season and fifth in six years. Brianne Barker earned her second consecutive All-America third team honor, while Boulavsky and McLaurin were named All-America honorable mention. Barker and Boulavsky earned first team All-Central Region honors from the AVCA and first team All-Big 12 honors, while McLaurin was named to the All-Central Region and All-Big 12 honorable mention teams. McLaurin was named Big 12 Defensive Player of the Year, while Carlee Roethlisberger was named Newcomer of the Year. Tara Dunn earned a spot on the All-Big 12 freshman team. Nine OU players earned academic all-conference honors.
The 2010 Sooners made the NCAA Championship and were awarded their first-ever first and second round host site as the team advanced to the third round for the third time in program history. The season was highlighted with a 23-11 overall mark and third-place finish in the Big 12 with a 13-7 record. McLaurin became the first Oklahoma player to be named Big 12 Freshman of the Year. Barker and Boulavsky earned first team All-Big 12 honors, while Barker was a first team All-Central Region selection and Boulavsky was honorable mention. Barker was also named to the All-America third team by the AVCA.
After losing his youngest son to leukemia prior to the start of the 2009 season, Restrepo returned from a leave of absence to lead his team to the NCAA Championship for the first time since the 2007 season. The Sooners finished fourth in the Big 12 after going 11-9 and 18-12 overall. Boulavsky earned AVCA honorable mention all-region honors. Barker and Francie Ekwerekwu were named honorable mention All-Big 12.
Restrepo coached Caitlin Higgins to All-Big 12 freshman team honors in 2008. She became the first OU player to earn AVCA Division I National Player of the Week on Nov. 4, 2008. McLaurin duplicated the feat on Sept. 17, 2013.
Selected ninth in the 2007 preseason poll, the Sooners advanced to the NCAA Championship, finishing 22-10 with a 13-7 mark in Big 12 play. Sarah Weiland represented OU on the AVCA and All-Big 12 honorable mention teams.
One of the most successful seasons in the program's history came during the 2006 campaign as OU turned in the biggest turnaround in Big 12 history. That year, Restrepo guided the Sooners to their first-ever win over Texas and the team advanced to the NCAA Championship third round for just the second time ever. Oklahoma finished the year ranked No. 12 in the final AVCA, its highest in history. Behind Restrepo's leadership, the Sooners recorded the best winning percentage in school history with a 28-6 (.824) record and finish second in the Big 12 with a 17-3 mark.
All five seniors on the 2006 team earned All-Big 12 recognition. Joanna Schmitt and Eliane Santos each earned All-America honors. Restrepo earned Big 12 and Regional Coach of the Year honors and was recognized by CVU.com as its 2006 National Coach of the Year.
Restrepo left Southern Mississippi in 2004 to fulfill a dream of coaching in the Big 12. In Restrepo's final season at Southern Miss, he led his 2003 team to a program-best 27-6 record and was named Conference USA Coach of the Year.
That squad reeled off three winning streaks of at least six matches, including one eight-win string, the longest in school history. Three Golden Eagle players garnered All-Conference USA honors during Restrepo's three-year tenure, including two in his final season. He left Southern Miss with a conference record of 52-40 (.565) and an overall mark of 127-72 (.638).
Perhaps the most remarkable part of the Golden Eagles' 27-win season is that it came just two years after a nine-win campaign. The 2001 team went 9-19 and finished 11th in C-USA. One recruiting class later, his 2002 team improved to 16-15 and finished the conference season in eighth.
Prior to coaching at Southern Miss, Restrepo served three seasons (1998-2000) as an assistant coach at Saint Louis University under Marilyn Nolen. Restrepo brought a tough training regimen to the Billiken program in 1998, and his dedication and work sparked an immediate improvement.
Saint Louis posted its fourth best single-season winning percentage in school history at 21-7 (.750) during the 1998 season. In 2000, he helped the Billikens to 23 victories, the most since 1996.
Restrepo served as the women's head coach at East Stroudsburg (Pa.) University from 1994-97. He led the Warriors to a 21-11 record and a semifinal finish in the Pennsylvania State Athletic Conference (PSAC) in his first season in the league. East Stroudsburg posted its best record in school history with a 32-2 mark in 1996. For his team's efforts, Restrepo was named PSAC Coach of the Year. During his tenure, he led ESU to a 75-32 (.735) overall record.
A former NCAA Division I All-American at East Stroudsburg, Restrepo was also a four-time all-conference selection. He earned team most valuable player honors all four years and was the co-winner of the senior athlete award in 1986. He was inducted into the East Stroudsburg Hall of Fame in 2004.
Following his collegiate career, Restrepo played one season professionally in France and returned to the United States where he played on the American Volleyball Players Pro Beach Tour.
He earned a 3A player rating on the AVP tour and had the opportunity to play against some of the giants of professional beach volleyball, including Karch Kiraly, Sinjin Smith, Randy Stoklos and Kent Steffes.
Highly involved with USA Volleyball and internationally, Restrepo served as co-head coach of one of two USA Volleyball under-22 select teams that toured Argentina in June 2005 and 2006. He was head coach of USA Volleyball's A2 junior national team during its summer 2011 international tour.
Restrepo and his wife, Heidi Nichols, have two sons, Diego Ignacio and Javier Santiago. Restrepo's youngest son, Javier Santiago, passed away from leukemia in July 2009 at the age of four. To find out what you can do to help promote childhood cancer awareness, visit The Children's Hospital at OU Medical Center.


text:
Show 2 sample images
Eine Kleine Nachtmusik (f.score)
Share with your friends
Price: $5.75
Available for sale by
Virtual Sheet Music
Virtual Sheet Music
Show Store Details
Tweet about this sheet music
for a chance to get it for free!
orAdd to wish list to buy later
This is an exclusive transcription for saxophone quartet of MEDIUM skill level from the original composition by Wolfgang Amadeus Mozart. This title is available as instant sheet music download. Contents: K 525, K.525, K. 525, K525, serenade,little night music
This sheet music has not received any reviews yet, you can be the first one to write one!
Virtual Sheet Music
Virtual Sheet Music has become one of the leading sources for classical sheet music downloads and for other music products and services serving over 20,000 users daily. Desired titles can be downloaded from the web site directly to the user's home computer, and subsequently displayed and/or printed. Users can also listen to and enjoy audio MIDI and MP3 files for any item in their catalog.
This store has not received any reviews yet, you can be the first one to write one!


text:
The Celestron Cheshire Collimation Eyepiece is ideal for precise collimation of Newtonians and helpful for aligning Schmidt-Cassegrains. This special eyepiece fits into 1¼" focusers or diagonals.
Alignment is easy using the small opening on one end and thin cross hairs at the other end. The collimation eyepiece is more accurate than the simple Collimation Tool.
Unfortunately our dealer agreements with Meade, Coronado, Celestron and Orion only permits us to ship their telescopes and accessories to addresses inside the United States of America.
No other images currently available


text:
Alison Brie
"Community" fans know her as Annie, and anyone who saw "Scream 4" saw Brie hit the big screen along with seemingly every other young actress in Hollywood. But 2012 could be Brie's breakout movie year with a major supporting role in "The Five-Year Engagement" (with Jason Segel and Emily Blunt) and a co-starring role in "Save the Date" (with Lizzy Caplan and Martin Starr). I'll be pretty disappointed if both of those movies aren't awesome.
Copyright © 2018, Sun Sentinel


text:
About hitZmagic.Com
hitZmagic.Com is an incentive based advertising community where the members will receive $0.07 for every ad they view for 30 seconds and also receive a percentage of what all their direct referrals earn. You can do the same now.
Advertisers need visitors and they are willing to pay you for it. Signing up is free and only takes seconds.
You don't need any skills!!
With hitZmagic.Com you don't need any skills. This is because all you need to do is visit the sites we provide you with.
How Much Can I Earn?
If you click 10 ads a day, you refer 100 members who click 10 ads a day, you could earn up to $3,500 yearly.
There is no limit in the amount of websites you visit or referrals that you obtain. With more referrals or ads the earning potential is endless. More about: More Info
Earn Money in Just 3 Easy Steps
You want to earn money in seconds with hitZmagic.Com?? Then View, Click, and GET PAID!!
hitZmagic.Com is a new innovative, international and FREE English based service that allows advertisers to reach thousands of potential customers by displaying their ad on our "View Ads" page..
You view websites for a few seconds, via the "View Ads" page. Once the few seconds is up, you'll either get a green tick sign or a red 'X'. The green tick sign means you've earned money for the visit and the 'X' means you have not earned money for the visit
Get Paid
If you have the minimum amount accumulated, you can click on your account balance within your stats area and it will submit your request.


text:
Lidstrom: Tecan Plate Reader
From OpenWetWare
Revision as of 10:18, 26 August 2013 by Janet B. Matsen (talk | contribs) (→Fluorescence-based promoter tests)
Back to Protocols
About the Tecan Infinite f500 plate reader
- This plate reader can do fluorescence tests.
- Product literature, machine specs.
Fluorescence-based promoter tests
- If you have promoters in front of a fluorescent protein and want to test the promoter strength. The answer you get will depend (nonlinearly!) on:
- The OD of the sample matters
- The gain you chose matters
- Should you subtract out fluorescence of the no-promoter control or divide the test value by the control?
- Evidence of both can be found in the literature
- You may have negative values for some samples.
- Is there a way to blank it??
- Evidence that the gain and the OD of the sample matter:
You should definitely:
- Compare the test to a control at the same OD.
- Compare across samples at the same OD
- If you are comparing different cell types, (e.g. E. coli compared to a small methylotroph) you can't totally compensate for effects of cell size, but using the same OD should help.
- This will require doing calculations for dilutions. You cannot just assume that 5uL of each type of cells + x uL water/buffer will give you the same OD.
- Use at least 50 uL (100 is better) so the 96-well plate has enough fluid
- Use the correct plate. Use one with black-sided wells & plastic that is compatible at your wavelength.
You should probably
- put cells in a non-fluorescent buffer.
- LB is known to contain fluorescent molecules.
Sample Protocol
See "Tips for Fluorescence Microplate Assays" in Invitrogen's Fluorescence Microplate Assays, 7th edition


text:
Past Media PartnersWe are delighted to have been associated with the following Media Partners for our past events. Please contact firstname.lastname@example.com if you would like to help partner and promote the event in 2015.
GolfPunk isn't just a magazine, it's a lifestyle. ... Read more >
Property Week
Property Week is the leading news magazine in the commercial property market. ... Read more >
TIME Magazine
Since its founding in 1923, TIME Magazine has been one of the most authoritative and informative guides to what is happening in the worlds of health and science, politics, business, society and entertainment. ... Read more >


text:
Norwegian Cruise Line's long-anticipated Epic has finally arrived, and it rocks.
Long an industry innovator with a list of firsts that includes the first alternate-dining restaurant at sea and a secluded complex of luxury villas atop the ship, NCL yet again breaks new ground in maritime entertainment, dining and ship design.
There is so much live entertainment aboard Epic, you won't want to put your head on a pillow until the wee hours.
Some of Epic's hallmark entertainment is unique: For example, you won't see the renowned Blue Man Group on any other ship. As usual, this trio of men in blue greasepaint were a howling hit.
In Fat City, something akin to a Greenwich Village cafe, you hear cool jazz.
It doesn't stop there: Other diversions include Chicago's Second City improv comedy; a Cirque du Soleil-inspired dinner theater with stunning acrobatics; dueling pianists, who toss songs to one another like hand grenades; and "Legends," a show with performers impersonating Elvis, Michael Jackson and Tina Turner. For a truly cool experience, there is even an Ice Bar.
NCL also stepped out of the box by transforming the boxy cabins typical aboard most vessels into curved-walled staterooms with a radical bathroom design that puts the bathroom sink outside the bathroom and the commode and shower behind matte glass but otherwise in the open and in your face — a mistake, according to many passengers.
Pioneers can expect to make a few mistakes.
What NCL did get spot on are its specially designed 128 studio cabins for single passengers. With most lines, singles must pay a premium for bunking alone. On Epic, they not only get 100 square feet of tastefully crafted cabins without surcharge but also a singles lounge for their exclusive use.
NCL also expanded on its already very popular Courtyard Villas concept, essentially an upscale small ship within a larger ship. It offers a private complex of 60 suites and penthouses on Epic's two uppermost decks. Here passengers share exclusive areas loaded with luxury options, such as their own dining area, nightclub and fitness center, plus a private pool deck area.
Despite all its novelty, including a huge water park, Epic probably makes its biggest splash with its refined eateries — some 20 dining options; nine of them require a surcharge ranging from $10 to $25.
Considering the quality of Epic's signature restaurants — the French Le Bistro, Cagney's Steakhouse, Italian La Cucina, Wasabi (sushi and sashimi) and Teppanyaki — passengers apparently do not object to an added tariff.
New to Epic are Moderno, an Argentine-style restaurant; Shanghai, a noodle bar with a la carte pricing; O'Sheehan's, an Irish pub with chicken pot pie and fish and chips on the menu (and a magnet for the 24/7 crowd); and the Manhattan Room, the closest thing Epic has to a main dining room.
Unfortunately, the success of these dining venues has unintended consequences.
Years ago, NCL introduced "FreeStyle" cruising as way of offering passengers more dining choices and the flexibility to choose where, when and with whom they want to dine, said Andy Stuart, NCL's executive vice president of global sales and passenger services. But Freestyle isn't always about spontaneity.
So popular are the specialty restaurants that it's often hard to pick a dining option without encountering either standing-room-only notices or long lines. To offset that, NCL does allow passengers to book restaurant reservations 45 days before departure.
Though Epic is huge, designers succeeded in seamlessly melding one area into another so passengers are led from restaurant, to lounge, to cafe, to theaters without need of a GPS.
With Epic, NCL took on some epic challenges and countered with some epic changes and once again set a new bar for entertainment and dining on the briny.
Ship stats
Passenger capacity: 4,100
Overall length: 1,081 feet
Maximum width: 133 feet
Cruise speed: 22 knots
Crew: 1,708
For a 7-night eastern Caribbean cruise sailing round trip from Miami, fares range from $679 per person, double occupancy, excluding taxes and port charges for an inside cabin, and $979 for a balcony stateroom, to $1289 for a spa suite. Studio cabins begin at $839, single occupancy. On some sailing dates, a free stateroom upgrade is included. Seven-night western Caribbean fares round trip from Miami may vary slightly from eastern Caribbean voyages.


text:
Today in A State of Trance 702 new tracks by Alexander Popov, Cosmic Gate, RAM, Bobina, Ronski Speed, ReOrder and many more! Also some news about ASOT Festival at Ultra Music Festival in Miami! Plus new Future Favorite and Tune of The Week! Enjoy! asot 702
Artist: Armin van Buuren
Title: A State of Trance 702
Date Of Release: 26-02-2015
Source: DI.fm (Trance Channel)
Genre: Trance
Quality: MP3 / Joint Stereo
Total Size: 270 Mb [SBD 320kbps]
Get ready to nice mix today!! For further info, when radio show starts and how to get broadcast links, please check on "About" page!
Read more… »
Tags: a state of trance 702, a state of trance 702 download, Armin van Buuren, ASOT 700 info, ASOT 700 news, asot 702, asot 702 cue, asot 702 Cue sheets, asot 702 download, asot 702 tracklist, Cue sheets for asot 702, state of trance 702, Ultra Music Festival 2015, Ultra Music Festival Miami


text:
When you've traveled too far to stay in a hotel that's interchangeable with the ones back home, there's often an option to stay in one worthy of writing home about.
The following ten lodgings are repurposed structures in settings like an Asian hillside, a Swedish peninsula, and a Costa Rican nature preserve. Their histories date back as far as the 14th century and involve famous authors, businessmen, and rock stars. Several of these buildings were drafted into service as shelters during World War II. Read on to see ten unusual hotels created in repurposed, and often abandoned, older structures.
By Colleen Kane
Posted 4 April 2013


text:
Offi & Company
Based in Corning, New York, OFFI & Company was born out of a love for great design. OFFI & Company satisfies this passion by creating simple, beautiful and practical furnishings that address the modern urban lifestyle. OFFI & Company products are created to be multifunctional, comfortable, and at home in any modern home in the world.


text:
About the Development Review Commission
The Development Review Commission is a seven-member citizen advisory body appointed by the City Council. It meets each month to review development applications for compliance with the City's land use and design regulations, based on criteria outlined in the City's Comprehensive Plan and Community Development Code and other related codes.
Development Review Commission Meetings
The commission meets on the 1st and 3rd Monday of each month at 7:00 p.m. in the Council Chamber at 380 A Avenue, Lake Oswego. The Council Chamber has disabled access. For any special accommodations, please contact Janice Bader at (503) 635-0297, at least 48 hours before the meeting.
The current commissioners are:
- David Poulson - Chair
- Brent Ahrend - Vice Chair
- Paden Prichard
- Nick Shur
- Jeff Shearer
- Kirk Smith
- Nader Taheri
Council Liaison - Theresa Kohlhoff
Back to the Planning Home Page


text:
News staff writer
October 7, 2006
The questions:
Each candidate was asked the same four questions. Some chose to make one statement while others chose to answer the questions individually.
Question 1 — What do you see as the top three issues facing Cascade Locks?
Question 2 — If you could change one thing about the way the city does business, what would it be?
Question 3 — In recent months, there has been acrimonious division with the council and community. How will you work in this environment?
Question 4 — Tell the community about your background, including biographical details and why you feel you are best suited for the position.
William K. Logue
My name is William K. Logue. I have been a Cascade Locks resident and property owner for 30 years.
I have decided to run for the mayor of Cascade Locks because I am concerned with some of the issues that are facing us. Among them are high utility rates, excessive spending, fire department issues and zoning concerns.
I feel that I can be of help in some of these areas. If I am elected I will be a "hands-on mayor." I will be available to hear your comments, complaints and ideas. My home phone number is 374-8887 and I will be available weekdays after 4 p.m. and most Saturdays and Sundays.
I would appreciate your vote.
Rob Brostoff
Question 1
The top three issues facing our city are:
One: The economy
Two: Public safety
Three: Housing
We need jobs, places to work, and for our children too. The resort/casino will certainly do that when and if it comes. The Port is also working on a proposed resort, but that is not a sure thing. Google is setting up a server farm in the Gorge. Microsoft and Yahoo will do the same. Google is already looking at another location in The Dalles. Other high-tech firms would find the Gorge an attractive place to call home. These firms are non-polluting and require only land, electricity and infrastructure; we have these.
We are ready to build our fire station. This will help us meet codes and rules, particularly OSHA's. The city council is asking for your support in forming a fire district to finance our fire and ambulance. The city has done this from its small budget for years, the equipment is old and unreliable; much needs to be replaced.
We need inexpensive housing: New housing in town runs between $215,000 and $315,000; not affordable by working people and our seniors. Could you buy your home at its current value?
Question 2
The past few years the city has raised millions of dollars in grant money. It's been raised by our administrator, Bob Willoughby, and the RARE volunteers he mentors and our fire chief. This is not a reliable funding source; in fact it's drying up, CLIK's inability to get re-funded is an example of this.
In addition, we have one person responsible for all our city bureaus and the fire and ambulance department, I feel it's too much for one person to be doing. In Portland each councilor is responsible for a bureau, spreading responsibility over several people and allowing for sharper focus.
The city should form a fire district, with citizens on the board to oversee the emergency services department. This will allow for more citizen input into the process. In addition, it will free up Bob to concentrate on other important functions in the city.
The emergency services department grows more important as our town grows; they need a stable source of funding that is not capricious. Over time the rules and regulations developed by different controlling agencies have made operation difficult without professional supervision. It's becoming important to bring professionalism to our fire and ambulance department.
Question 3
There are many schisms in our community and county. I ran for county commissioner in hopes of healing some of these. It's important to heal them on the local level also. Reaching out to those with different ideas and finding common ground is a start. Working on common projects and developing trust helps. Developing an inclusive, not an exclusive attitude makes people feel welcome. Building trust and consensus helps. Getting out into the community and looking at what people want goes a long way to build bridges.
As a mayor, it's important to set expectations, both for the council and for the community. For the council, it can be important to hold workshops to explain what can and can't be done, how things work, build trust and learn to work together for the common good. Staff plays an important role in this; they do the daily work. It helps for key staff to set expectations and explain what their role is and how it works.
Holding town halls on issues important to the city gives people input into the process. These are basic ways to start to heal the divisions within our city.
Question 4
I grew up in the Portland/Vancouver area; my spare time was spent in the Gorge and on the Columbia — my favorite places.
At 22 I went into business in Eugene, a pizza parlor named Rico's.
My degree is in electronics engineering technology; I went to work for Prime Computer in 1982 in the Bay area. I worked as a factory field engineer there, and for Apollo Computer; later acquired by Hewlett Packard. I repaired, installed and taught hardware and software to customers and fellow engineers.
Retired at 54, I moved to the Gorge where I reside. Since coming to Cascade Locks I've been in the Lions, on the planning commission, and the city council for six years. Also the city and port budget committees.
I've mentored elementary children in chess at our school, and organizing tournaments. I served as Cascade Locks rep on the advisory committee to the school board; we built the music room along with rebuilding the locker and shower rooms, nearly $1 million's worth of construction.
As part of the telecom committee, we brought you broadband, improved your TV signal, and lineup.
These accomplishments speak for themselves, and hopefully will be a strong predictor of future accomplishments.
Arni Kononen
Question 1
The top issue I see is what I call "Smart Growth." Cascade Locks is experiencing a growth spurt. That growth is happening with or without the casino. We have to be smart about how this growth happens. How will the traffic patterns change? Do we have the right infrastructure in place? How can we keep our city's character? We only get one chance to do this right.
The second issue is: How do we ensure that fire and emergency services are staffed and equipped properly? We need equipment now. The current fire hall is woefully inadequate. New trucks will not fit in the building. Providing adequate and stable funding for emergency services could be a life-and-death issue for some citizens. We have to make sure they have what they need.
The last issue is the city's sense of community. We need to be a community that takes care of each other. We are a changing community. We have some who want to go in one direction and some in another. We need to put our heads together and see if we can come up with solutions that are satisfactory to all so everyone can enjoy their city.
Question 2
I would change the way the City Council communicates with residents. Some say Cascade Locks does a wonderful job by televising council meetings and sending out a quarterly newsletter, but those steps are not enough.
The Council often passes resolutions or ordinances with complicated titles and with little public discussion except in un-televised workshops. A person watching the council meeting has no idea what their council just passed. If a citizen then tries to find the ordinance on the city Web site, they are met by an unforgiving search engine that confounds and confuses them. It certainly frustrates me, and I know what I'm looking for.
We need to have more citizen input into future council actions. We need to let citizens know what we are doing and why. They need to hear the discussions and not just the vote. Several recent controversies could have been avoided if residents had been aware of the Council's intentions.
Question 3
Communication is again the key. When a community is changing, there is often resistance to change. Sometimes we have choices and sometimes we don't. We can try to mitigate problems, though. When reasonable people discuss all the options, solutions are usually found. The entire community hasn't been upset with the Council, only a small part of the community. If the council had communicated with them properly, they might not have been so upset. That doesn't mean they don't have legitimate concerns or that those concerns should be ignored. We need to hear all concerns. It only takes one person to find a fatal flaw in a plan that everyone thought was great, and we should be glad that one person found the flaw for us. Anger and harsh words won't solve problems. Communication, discussion and brainstorming are better tools for problem-solving.
Question 4
I've been a resident of Cascade Locks for approximately 18 years. My wife and I have seven children, so I understand family problems and will continue to work to strengthen families as city policy. I work for the State of Oregon Department of Human Services. I've been a member of the Cascade Locks Budget Committee and I currently am a member of the City Council. As a Council member I have not been afraid to challenge current policies or practices such as my recent objection to a power rate increase I felt we didn't need. I believe in fairness for all and will work in the best interest of the entire city. I want the city to become more self-sufficient. I want to see more local jobs. I want to see better communication between the Council and the citizens. One way I intend to do this is to energize the City's committees so more residents are involved and have a greater say in their community. I hope to give the city a positive image so we can be proud to be Lockians.
Roger Freeborn
Question 1
1. Present and future growth must be immediately revisited. Although we have a well-thought-out Comprehensive Growth Plan, developed five years ago, addressing housing and our downtown area, recent residential development and the lack of downtown improvements have raised questions. I believe most citizens' concerns can be addressed through forming a citizens' advisory committee coupled with public hearings to amend our current Plan to suit the mindset of the citizens at this time; no doubt, the Plan will need revisiting as growth continues.
2. The city, public servants and elected officials have, for the most part, lost touch with the citizens of Cascade Locks and the will of these citizens. As elected officials, we must remember that we are the voices of the majority of voters. It is an elected official's duty to stay in touch with constituents. The staff must remember that they serve at the will of these elected officials. I do encourage staff to think creatively. I also believe citizens have a responsibility to contact their elected officials with their thoughts. Had this simple scenario been followed recently with the sale of the McCoy property and the current attempt to form a fire district a whole lot of bad feelings could have been avoided.
3. The size and control of government in Cascade Locks. Our lives are controlled by the actions of a city, port, county, ODOT, Forest Service and possible Fire District. Government is big business in C.L. I would like to try to lessen government's effect on our lives in C.L. and make it work for us instead of against us.
Question 2
If I can change one thing about the way the city does business: financial accountability — take away any doubt of the citizens that their city government is not squandering money. We are a town of approximately 1,000 people; to put that in perspective, the size of a large apartment complex. We purchase all of our essential services from the city or county and some non-essential services through either taxes or monthly billing. I believe that in a few areas, we are living beyond our means and the city has only a general accountability. I know that any government staff hates this but the new council must implement some micro-management and cost analysis of certain government services to rein in spending or at least have an accurate accounting of department expenditures. A case in point: The city auditor for years recommended that the clerical department have a work order system in place to track expenses; to this date no system is in place other than general government budgeting.
Question 3
I realize that the new mayor and council have their work cut out for them bridging the communication gap that has festered in recent months between the city, council and citizens; how would I work in this environment? Listen, listen and listen some more; when I feel myself and the council have all the facts I would act to best serve the will of the people.
Question 4
I have been elected to this office twice before, fulfilling one term and having to resign shortly into my second term due to a bout with cancer. I have now sat on the sidelines for about three years, observing my local government in action, and have learned a lot by watching. I feel that having been there, serving with a fairly diverse and successful Council and now having observed another Council gives me a unique perspective of how this city should be governed. I have served on the city's budget and tourism committees. I have been party to negotiations concerning a casino, electric rates, water and sewer rates, hiring city and county staff, Dry Creek low-impact Hydro project, Comprehensive plan review and I have worked with two separate governor's staffs to mention a few. I spearheaded the formation of the Cascade Locks Drug-Free Zone and created what I consider the town's largest event, the Cascade Locks Kart Grand Prix. I also started what I believe to be the first CL Mayor's Ball. I am involved in the construction industry, car sales, and event promotion and management. It is these life experiences, and more, that make me feel I am qualified to serve as mayor.
Bob Walker
Question 1
One: Restoring the community's confidence in the mayor, the City Council and city administration.
Two: Assure that our high school not only remains in Cascade Locks, but that it will also grow and improve.
Three: Come to a consensus on what the citizens really want Cascade Locks to look like not only today but in 10, 20 or 30 years.
Question 2
Our local cable channel is virtually unchanged from what it was 25 years ago. I believe it is underutilized. As mayor I will explore ways to maximize its use as a source for information-sharing for city-related activities, school and the rest of the community. I think this can be done with very little or no cost to taxpayers. Perhaps we can partner with the high school? Students could earn credit for producing informational videos and videos of events of interest to all of us.
The city Web site is another area where there is a lot of room for improvement. Much of the information found there is not updated. People gather and share information online — that's the way things are done now and we need to do a better job of making information available.
If there is a meeting of the City Council or Planning Commission Monday, I want the minutes of that meeting to be available on our city Web site by Thursday.
The city newsletter latest edition appears to have become comprised more of opinions than actual fact and useful information. It could be published more often.
I believe that by working harder on sharing information, we can better deal with the three issues I have identified.
Question 3
The level of dissatisfaction with our city government is easily recognized. In my 40 years of living in Cascade Locks, I can't remember another time that five people were running for mayor of Cascade Locks. The recall effort against two council people is another indication that a portion of the community feels that their opinions are not being seriously considered. I believe we can begin to solve the problem of confidence in our city government by making information more accessible. People are complaining that they are not being listened to. No reasonable person can expect to get things their way all the time but when unpopular and difficult decisions are made, are the city council and mayor making every effort to give people all the same information as the Council has?
We need to communicate better so everyone can see all sides of the issues.
Question 4
I have lived in the Gorge for all but one year of my life. My family moved here from Bridal Veil in 1967. My grandfather and father worked in the mill in Bridal Veil back in the days when the mill was owned by the Kraft family and they made boxes, mouse traps and window and door frames. I attended Cascade Locks School and graduated in 1973. I married Myra Klapprich after she graduated here in 1974. I have two sons, a daughter, three granddaughters and one grandson. In the past I have served on the Cascade Locks Fire Department as Assistant Chief, served on the City Budget Committee, and I was on the Local School Committee. I have coached several kids' sports teams at the youth and high school levels.
I have been employed with the Oregon Department of Transportation since 1993. I worked my way up to Supervisor of the ODOT Region One Sign Crew, which I managed for six years.
In January 2005 I was promoted to Assistant District Manager of ODOT District 2B. My work in state government has enabled me to gain valuable experience in dealing with regulations, contract maintenance, purchasing, dispute resolution and being a servant of the public.
Comments are subject to moderator review and may not appear immediately on the site. A user's first several comments must be manually approved by a moderator.
Please read our commenting policy before posting.
Use the comment form below to begin a discussion about this content.
Information from the News and our advertisers (Want to add your business to this to this feed?)


text:
Event DetailsFebruary 08, 2016 | 03:00 PM
Blue Buffalo is taking the stampede to the streets and will be at Pet Supplies Plus! We're on a tour across the country meeting pet parents, spreading the good word about natural nutrition, and making some new best friends, too! Check out our tour schedule and stop by when we're in your area. We'll be giving away coupons, treats and more!
Pet Supplies Plus
4923 Sheridan St, Hollywood , FL 33021
4923 Sheridan St, Hollywood , FL 33021


text:
From FamiLAB Wiki
Early geek development, circa 1977
Tracy Lunquist
IRC nick= artemisia
My geekdom is mostly education -- I know a lot about teaching, facilitation, training, development and the differences between all of them. I know quite a bit about how organizations work and some reasons why they don't. As FamiLAB evolves into needing things like a mission statement, a long-term plan, member orientation classes, policies and procedures, I can get my geek on about all that stuff and help make it work really well.
I have also earned my accomplishment patches for soldering and letting the magic smoke out of an IC.


text:
NORFOLK — Citing the need for greater leadership for its players, Old Dominion athletic director Wood Selig fired men's basketball coach Blaine Taylor on Tuesday, the latest blow in an epically dismal season for one of the nation's notable mid-major programs.
Taylor, the most successful men's coach in ODU history, was fired just hours after an 85-74 loss to George Mason that dropped the team's record to 2-20. The Monarchs are spiraling toward the program's worst season in the Division I era after a run that included seven 20-win seasons and four NCAA tournament appearances in the past eight years.
"This decision is not based solely on wins and losses, but a number of factors by which a head coach is evaluated," a stone-faced Selig said Tuesday afternoon, reading from a prepared statement in the press room of the Constant Center.
"At this point, in regard to our head men's basketball coach, our student-athletes need more mentorship, guidance and leadership," Selig continued. "Our fans and alumni need encouragement. Our administration needs confidence in our leadership."
Selig named longtime assistant Jim Corrigan as interim head coach for the remainder of the season, which ends March 2 at Northeastern. In their final season as members of the Colonial Athletic Association before leaving for Conference USA, the Monarchs are not permitted to compete in next month's CAA tournament.
Selig was accompanied by ODU president John Broderick, a fixture at Monarchs' basketball games and athletic events. Broderick said that he supported Selig's decision.
The two men sat at a table in the front of the press room for several minutes and made statements before departing without taking questions.
Senior associate athletic director Debbie White said that neither Selig nor school officials could elaborate on Taylor's dismissal because it was "a personnel matter." She spent approximately four minutes fielding questions, most of which she said that she could not answer.
Selig had told reporters recently that he fully expected ODU would return to previous success enjoyed under Taylor. Asked what had changed in the past couple of weeks, White declined comment.
White said she wasn't permitted to discuss if Taylor would be paid for the final two years of his current contract. She did say that Taylor was told he was fired late Tuesday morning. The coaching staff and players were informed early in the afternoon.
None of the remaining staff or players was available for comment. No practice was scheduled Tuesday, so the players were not at the Constant Center. The Monarchs next play Thursday at Drexel. Their next home game is Saturday versus league leader Northeastern.
Hampton High guard Jordan Baker is one of three players who have committed to the Monarchs for next season, and he said that Taylor's dismissal hit him hard.
"It's just sinking in," Baker told DP colleague Dave Johnson. "I'm really shocked. I thought at least he'd get another year for my group to get there, but the AD thought otherwise. It kind of hurts, but Coach (John) Richardson called and told me to only worry about Hampton High School basketball right now. He said he and the assistants will be there for me on the road to another championship."
Baker said that he planned to honor his commitment to ODU out of loyalty to Corrigan and Richardson, who were his primary recruiters. But he said that Taylor played a major role in his decision.
"We sat down face-to-face and he offered me the scholarship," Baker said. "Then we sat down again face-to-face and I accepted. The whole atmosphere has a family feel and there's going to be a hole there."
Taylor, 55, is one of the glib and gregarious figures in the history of ODU athletics. The native Montanan combined folksy, homespun humor with a fierce, demanding and at-times profane style that succeeded at the three places he's coached: Montana, Stanford and ODU.
He leaves ODU as the school's winningest basketball coach, with a 239-144 record in nearly 12 seasons. He revived a program that had dipped under previous coach Jeff Capel. He bridged the gap between the program's traditional homes of Scope and the ODU Field House, and the on-campus Constant Center, which opened in 2002.
The Monarchs were sub-.500 his first two seasons before gaining traction. The breakthrough came in 2004-05, when the team went 28-6, won its first CAA title since 1997 and carried powerful Michigan State to the end before losing in the first round of the NCAA tournament.
That began a string of seven 20-win seasons in eight years, with three CAA titles, four NCAA appearances and an NIT final four.
Selig praised Taylor and his family for their contributions to the ODU campus and community. Indeed, Selig said recently that Taylor was one of the school's most effective fundraisers. He raised hundreds of thousands of dollars through the years to endow scholarships and for the program. He helped elevate the school's profile throughout the community.
"Blaine's student-athletes have always shown great character and tremendous heart," Selig said. "They have enjoyed considerable athletic and academic success. They have gone on to become productive citizens in their communities following graduation from the university.
"In those regards, Blaine has led a model program — all of which has made for a difficult decision today. But as previously stated, my primary focus is our student-athletes and the future of our men's basketball program."
Asked about the peculiarity of terminating a head coach with just eight games remaining in the season, White responded, "I can't comment any further than to tell you that I think you can look at the expressions on people's faces to understand how difficult a decision this was."
Selig insisted in his statement that, "We are not giving up on our student-athletes or the program. Today's announcement is about addressing the present and the future needs of our men's basketball program. We want to make the most of our remaining games — there are eight games left — and we want to put our student-athletes in position for success."


text:
Swollen Members - Death Warrant
Music video from Swollen Members from the new album "Beautiful Death Machine" out now
Posted by Jay
Please click the "Report" button below if the video on this page is not working properly.
Comments will only be displayed by verified emails. No Spamming or Racism.


text:
Information regarding both Federal and State of Wisconsin workplace poster requirements can be found at the Wisconsin Department of Workforce Development web address below. To order Federal posters, use the Department of Labor web address. Almost all posters can be printed directly off one of these two sites using the Adobe Acrobat Reader format.
U.S. Department of Labor Posters
Wisconsin Department of Workforce Development Workplace Posters
questions about this page, or accessibility issues: firstname.lastname@example.com
Published March 21, 2005


text:
Gym Allegedly Threatens To Call Police On Blogger For Blogging That Its Prices Were A Bit High
from the good-luck-there dept
Alana Munro's original blog post did discuss the fact that she thought the price was too high, and that she normally likes a workout that generates more of a sweat, but on the whole it doesn't seem that negative at all. She noted that if it was a little closer to where she lived (and the prices were lower) she'd likely go back. However, somewhere along the way that got interpreted as being a dig by someone who ran the place, and they first got into a bit of a Facebook and Twitter argument with Alana and some others.
And then.... someone with the same last name as the owner posted a comment on the original blog post, accusing Alana of "stealing" the class she attended:
Just stop the posting about Barre Cleveland and take down all the existing posts. We know that you stole the class and we can pursue legal action against you for that and that is why it is ridiculous that you complain about a price when you never paid for the class. You were never given a discount code by Barre Cleveland and somehow you used that to enter the studio. I am sending you this message to politely ask that you remove all the content about Barre Cleveland from your blog and twitter and we will not get the Beachwood Police involved on this theft of services.Alana says she did use a discount code -- it was one that was being passed around widely to help promote the gym in the first place. Now the gym may argue that it only meant the code to be used by people it gave it too, but then it should have implemented one-time codes. Instead, the code worked, and Alana has a nice receipt posted to her blog "thanking" her for signing up. Her complaint wasn't that she paid too much for that one class, but that the general price might be too much for her to keep going back.
Either way, the whole thing has blown up and received lots of attention. BarreCleveland has scrubbed the back-and-forth tweets from its Twitter feed. But it certainly looks like it already went way too far. Engaging with people who criticize you online is one thing -- and can be quite useful. Threatening to call the police on them and demanding they erase what they've written about you is going way too far, and the internet (hello Streisand Effect) doesn't take kindly to such things.