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|XP is just a number
Re: passing env{} variable to child fails on linux,works on windowsby flexvault (Monsignor)
|on Jan 12, 2013 at 16:48 UTC ( #1013052=note: print w/replies, xml )
|Need Help??
Hello sdetweil,
Since you are testing for MS and treating the problem different for *nix and MS, you may not know about '/dev/shm' which most modern *nix have. If you do a 'df' you'll see if it's there. What this does is allow you to share information between different processes in *nix. It treats memory as a separate filesystem for you to 'open/read/write/close' in memory files without going to disk.
In my testing, I've found it to be 2 or more times faster than using disk, and it's 'cleaned-up' on re-boot.
I don't think the method you're using in *nix will work, since there is no relationship between the process created with 'open' and your process. Why it works in MS, someone else will have to explain. If you use 'fork', all variables are copied to the new process, so you can say:
and it will be available in the new 'fork'ed process.
Good Luck!
"Well done is better than well said." - Benjamin Franklin
In Section Seekers of Perl Wisdom


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NORTH BROOKFIELD — A 46-year-old bus driver, helping her lone passenger disembark from the back of a small bus Wednesday afternoon, was struck and killed when the bus started moving, according to Worcester District Attorney Joseph D. Early Jr.
Nancy M. Woods of 176 Maple St., Rutland, was taken to Harrington Hospital in Southbridge, where she was pronounced dead.
Witnesses said the Worcester Regional Transit Authority bus stopped near Chestnut and Maple streets about 3 p.m. Ms. Woods was helping a passenger get off the vehicle from the back of the bus when it started to move forward, the district attorney's office said in a news release.
Witnesses said Ms. Woods ran around to the driver's side door and reached inside, but the bus struck her and continued to roll down Chestnut Street.
The bus, which is the size of a large van, continued to roll until it struck a garage at 74 Maple St. The stairs and porch for an apartment over the garage were damaged. The passenger, 45, remained in the vehicle and sustained minor injuries. The investigation is continuing, the district attorney said, and no further information was available Wednesday night.

subset: commoncrawl


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NEW YORK (AP) — Scottish author and playwright J.M. Barrie, creator of Peter Pan and Neverland, also wrote intelligent comedies for adults confounded by the fast-changing Edwardian era.
The Pearl Theatre is closing out its 29th season with a lighthearted pair of Barrie's plays, combined in "This Side of Neverland" and deftly staged by Pearl artistic director J. R. Sullivan.
Sullivan creates a period atmosphere of breezy charm in the winsome production, which features several members of the Pearl's resident acting company who are expert at insouciant, perceptive farce. The show opened Sunday night for a brief run at the Pearl's new permanent home on far West 42nd Street.
Prior to the first play, the audience is invited to sing along with engaging turn-of-the-century songs played on an upright piano by Carol Schultz. In the lyrics, courting couples ride on bicycles built for two, chase rainbows and dance waltzes while the band plays on.
Rapidly leaving behind those innocent concepts, the theme in the comedies that follow is more realistic. In a period of rapid social change, women were beginning to find personal freedom, empowering themselves while leaving generally confused menfolk in their wake.
Sean McNall suavely enacts Barrie, introducing each play by reciting the extensive stage directions.
Rachel Botchan's eyes sparkle with mischief in "Rosalind," as she portrays middle-aged Mrs. Page, the retired mother of an actress who lazily gossips with her landlady (Schultz.) When McNall intrudes as a hopeful, naive suitor of her daughter, Mrs. Page gives him quite a surprise.
In the second play, "The Twelve Pound Look," Botchan stars again, this time emanating poise and confidence as a typist named Kate. Her visit to a smug, shallow man about to be knighted will radically change his selfish, well-ordered world. Bradford Cover preens with swaggering pride as soon-to-be-Sir Harry Sims, with Vaishnavi Sharma sweetly demure as his politely loyal wife.
Kate merrily greets all of Sir Harry's boastful claims of success with barely concealed mirth and impertinence. The audience laughs along with Botchan, especially when she brightly agrees with Sims' clearly inaccurate opinion that he has "a profound understanding of women."
Getting a bit closer to the magic of Neverland, it may be improbable that these women successfully pull off their various bids for freedom in a male-dominated world. But that's the charm of Barrie. He made us believe in Captain Hook and Tinkerbell, and so we can believe that women oppressed by Victorian strictures could find the spirit to escape their gilded birdcages of propriety.


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Reviewed by Diana K. (age 10)
I like this story because Jamie had to see how hard it is to work, because his wife did it all. Also you will learn about a new word, "pratie". It means potato. It is good to read this story because the man changed. He started to work a lot harder.
I recommend this book to all children. Everyone can learn that it is important to work hard.


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Cold Case on the Missing Person of Terry Turner
Detectives at the London Post are asking for your assistance in a missing person case from 1992.
Last Seen 9/25/1992
Last Seen ago.
On September 25, 1992, Terry Turner left TURNER'S GUN SHOP and has not been seen since. Terry Turner owns this gun shop and left the gun store that day around 1:30 p.m. According to an employee at the gun shop Mr. Turner left with someone in a vehicle but the employee could not see the person in the vehicle.
Mr. Turner was last seen wearing a short sleeve shirt with a camouflage vest, blue jeans and white tennis shoes.
The victim's family stated that Mr. Turner had an alcohol problem and the family believed he may have gone somewhere seeking help. However, checks of area facilities turned up no information.
If you have any information in this case, please contact Lt. John Yates or Sgt. Greg Hill at (606) 878-6622 or you can email them at email@example.com or email@example.com with any information.
Investigating Officer Contact Information
OFFICERS: Lt. John Yates or Sgt. Greg Hill
firstname.lastname@example.com or email@example.com
If you wish to report more information on this Cold Case, use the form below.
However, if you need to report information that is of an emergency nature, please contact the Kentucky State Police toll free at 1-800-222-5555 (in the state of Kentucky only, does not work out of state) or call your local police/sheriff department at 911.


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I thought I had over supply but now we have hit a growth spurt and by tonight i feel totally empty and she is screaming and coming off the boob. When i try to express i get almost nothing. should i be freaking out now?
I thought I had over supply but now we have hit a growth spurt and by tonight i feel totally empty and she is screaming and coming off the boob. When i try to express i get almost nothing. should i be freaking out now?
should mention that i have not been pumping regularly as i feel her always on demand, she is 8 weeks old. she has never had a bottle but i am wondering right now if i need to offer her one with milk i pumped and had frozen from her first month.
Your breasts are never empty. They may feel that way at the end of the day - when milk supply tends to be less - but there's milk in there. It's common for an 8 week old to be fussy in the evening. Offer the breast; if she doesn't want it, you can always offer again a little while later. Assuming that everything is on track with baby's weight gain and diaper output, no need to offer frozen milk.
Normally there is no reason to pump in addition to breastfeeding on demand if you are not separated from baby. However, pumping in addition to breastfeeding on demand is done under certain situations. Have there been any problems with baby's growth? How are baby's diapers?
Tips for helping with a fussy baby:
Make sure baby is burped and has a clean diaper
Motion - rocking, bouncing on an exercise ball, walking with baby in a carrier or pushing baby in a stroller
Decrease stimuli - lights, noise, etc; sing something soothing and monotonous to baby
Keep baby close
Sometimes no matter what you try baby will be fussy! Usually babies grow out of this by about 3 months or so, but some babies are more sensitive than others.
I am used to that kind of fussiness but this is a new thing for us and not the same as it was when it was the "colic" fussiness as far as i can tell. she is getting only mad when she is showing signs of hunger latching on and then getting little to nothing. at first she was just annoyed and now with time and each offering she is getting more and more angry. This has just been today so I don't think it is far enough along for her to have started having dry diapers or big weight loss. Just wondering if when she is suddenly eating every hour the whole day long, if it depletes your milk supply and if there is anything you can or should do about this.
How do you know she is getting little or nothing? Why would this happen? It does not make sense. A womans body does not just stop producing milk for no reason. UNLESS you have started on hormonal based birth control That can possibly cause production issues.she is showing signs of hunger latching on and then getting little to nothing.
Baby wanting to nurse a lot or being fussy is in general, nothing to worry about. It's totally normal. Roll with it and let baby nurse, this is how you and baby together keep your producton in good shape for growth spurts. If she won't nurse, calm her some other way down and try again later. Also Try to nurse at earliest cues before she gets upset.
You're doing great. Keep up the good work!
"We are not put on earth for ourselves, but are placed here for each other. If you are there always for others, then in time of need, someone will be there for you."
Thanks so much ladies! I assumes she wasn't getting anything cause after she let go and i tried to express i got nothing. I just got super scared, ask my hubby I over think and worry big time lol. By this morning it seems to be ok. My boobs still feel super light which seems so odd (especially for morning!) but she is eating normally again, if a lot still hehe. In the moment I was so just taken aback because usually even at night I can get milk to squirt out with ease. I think it must just be my boobs finally evening out and not making way too much and that scared me hehe.
OH and I did just start the depo 3 month shot. (almost 2 weeks ago) The gyno told me that it was ok to use while breastfeeding....that wouldnt have caused my supply to start dropping or anything would it? If yes what can I do about it cause I have to wait now to get it out of my system if i were to need to stop taking it, which i would in order to breastfeed with ease.
Your doc is on crack. Depo is NOT okay for breastfeeding. Some moms will experience a large decrease in supply after getting the injection, and unlike every other hormonal contraceptive on the market, you can't stop taking it (like the pill) or have it removed (like the hormonal IUD). You just have to wait for it to wear off. That takes 3 months, I think?
Okay, since there's nothing you can do at this point except wait for the shot to wear off, how do you manage the situation in the meantime? The first thing you do is nurse on demand, no matter how often or how long the baby seems to want. The second thing you do is to keep a close eye on diaper output and weight gain. If either of those things happen to drop below normal, you may need to supplement. But cross that particular bridge when you come to it. It's quite possible that your supply is still enough for baby.
Finally, when the time comes and the shot wears off, don't go and get a repeat!!! There are plenty of contraceptive choices which are safe for breastfeeding, and you can always run them by the forum.
OK, wow about the depo shot. PLEASE I beg you, tell your doctor they are wrong to tell mothers it is "OK" to take something while breastfeeding
that has been proven to harm milk production!!!!!!!!!!!!!!!! EDIT-NO, I am wrong, this has not been proven if the shot is given after 6 weeks (Although there is still evidence it does.) Please see my later posts
For ACCURATE info on hormone based birth control methods and breastfeeding, you can also call these folks. www.infantrisk.org
In fact I would suggest calling them anyway to talk about what happened here and see if they have anything to add to mommals suggestions, which I think are good.
It will be ok. I agree with mommal about how to handle this. Luckily, you had normal milk production established by the time yuou had the shot (at about 6 weeks of age, correct?) which I think will lessen the impact of this. Mothers are given depo shot in the hospital sometimes right after birth and that is really tragic. I am just so sad thinking about all the mothers and babies who are being adversely affected by hormonal birth control when there are so many perfectly safe and effective bc options. It's infuriating!
Last edited by @llli*lllmeg; May 18th, 2013 at 10:13 AM.


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uptempo wrote:Bogut gives this team an interior defensive presence which it has not had since Nate Thurmond.
I do like how Bogut plays strong help-defense (desperately needed by this team). My only concern was his one-on-one defense vs. Cousins in the post; but then again, Cousins is a beast.
As he gets healthier and stronger, I can only see Bogut's contributions on both ends of the court increasing. I also like Ezeli as his backup, even with his bad hands; the kid plays tough defense and can rebound.
My sleeper player on this roster is Jeremy Tyler.
Do others on this board sense that this Warrior team may actually be quite good and can compete against any team in the league? While the team may not be a championship contending team, I actually see this team improving and possibly making the playoffs.
Ageed, old friend. Bogut is the missing piece this team has sorely lacked for decades. Big, mean, and merciless on the defensive end.
I also noticed Ezeli dropping passes, but to my mind it seemed like a one-game affair. He showed great poise finishing plays both in the preseason and against Memphis. We're a ways away from me labeling him with bad hands (like old man Foyle and his clumsy stone mits).
You have more faith in Tyler than I do. Perhaps I just burned myself out rooting for project big men like Randolph, Wright, Diogu, Biedrins, and O'Bryant... But Tyler seems like another AR to me. If he used his size to rebound and block shots, that'd be one thing, but he throws the ball away a lot because he wants to operate like Carl Landry and he doesn't have the skill yet. He's an athletic freak, there's no doubt, but with Bogut and Landry on board, I've relegated Tyler to an afterthought.
What do you think of Draymond Green so far?


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Style # 214156
Smocked detail that cinches at the waist, wrapped design and sharply cut neckline make this bebe top a polished way to incorporate color into your look. Try it with suiting pieces to wake them up.
- 100% polyester
- Center back to hem: 17" (43 cm)
- Hand wash
- Imported
- Model is 5'9" and is wearing a US size S


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It's a complete song in the style of power metal without vocals.
This is a epic song with a melodic foundation and a powerful hard guitar riffs.
In this composition, I used a synthesizer. The main theme is realized on it.
This track has become faster and more melodic than my previous work.
Ideal for videos showing extreme, speed, flight and various epic scenes.
Well ideal for video games like a shooter or a race etc.


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what does that mean? i just installed windows and set up my internet connection through a lan and the icon for it has a lock on it. here's a screenshot http://img153.exs.cx/img153/374/lanlock0ui.jpg
Look carefully at the description "Connected, Firewalled"
Yeah thats all, that means windows firewall is enabled for that connection. If you have your own 3rd party firewall dont forget to disable windows built in firewall my freind.
Some helpful links:
"Data is not necessarily information. Information does not necessarily lead to knowledge. And knowledge is not always sufficient to discover truth and breed wisdom." --Spaf
Anyone who is capable of getting themselves made president should on no account be allowed to do the job. --Douglas Adams (1952-2001)
"...people find it far easier to forgive others for being wrong than being right." - Albus Percival Wulfric Brian Dumbledore
i thought i had windows firewall turned off though
nvm, it goes away when i turn the fw off they should really replace that lock with the little windows fw shield symbol since a lock usually implies that you dont have priveliges.
Forum Rules


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Howard Hanna Convention and Annual Awards Show - 2/23/10
A two-day convention brought together more than 2,000 Howard Hanna Real Estate agents and managers to learn the tools for claiming victory in the workplace. The 2010 Convention and Annual Awards Show, titled "The Keys to Your Success," was held at the David L. Lawrence Convention Center and featured a keynote speaker, educational courses, panel discussions, and entertainment for participants. Addressing the company for the first time was Howard Hanna CEO Hoddy Hanna. The featured keynote speaker, Dr. Kevin Frieberg, coauthor of BOOM — 7 Choices for Blowing the Doors Off Business-as-Usual, presented on the first day of the convention. The first day of the event wrapped up with an evening cocktail party and a performance by musical act Solid Gold, followed by additional informative breakout sessions and panel discussions the next day. Photography by Megan WylieRead More
13 / 29


text: Story Posted 01-16-2013 Been Here, Done That But Obama's new gun proposals draw fire at SHOT Show A crowd at the SHOT Show watches Obama's address. By: Steve Wright, OutdoorChannel.com LAS VEGAS — That recent two-word curse Rep. John Boehner directed at Sen. Harry Reid during the fiscal cliff negotiations was repeated en masse Wednesday on the main floor of the SHOT Show. No, those particular words weren't shouted in refrain to President Barack Obama's message. But neither are they a gross exaggeration of the reaction. This group is upset. A perceived restriction of constitutional rights coupled with a threat to your economic survival smacks a powerful one-two punch. President Obama's address on proposed gun safety laws was streamed live on a football stadium-size video screen above main showroom floor, where representatives of the gun industry are gathered for the 35th annual trade show. You didn't have to take many steps in the 12.5 miles of aisles at the Sands Expo and Convention Center to hear the vitriol in reaction to the President's words. History, however, probably deserves more attention now than any reactions from today. "Those who cannot remember the past are condemned to repeat it," as American philosopher George Santayana once said. With the 1994 Federal Assault Weapons Ban, which expired in 2004, as the primary history lesson here, the latest proposals from the White House are more likely to stimulate the gun industry than stifle it. While the President offered some ideas previously untried, the assault weapons ban and magazine restrictions? Been there, done that. A Tuesday conversation with Jim Drager, head of business development at Mission Arms Group, proved even more enlightening after the President's address than it had the day before. In the calm before the storm, Drager offered his thoughts on what might be coming. "The more they try to restrict these guns, the more people want them," Drager said. "That's just the facts, not opinion. Before that (1994 ban), some of these guns were kind of viewed as an extremist weapon. But people said, 'Hey, nobody is going to tell me I can't have these,' so they started buying them. "A lot of the mystique of them being 'assault weapons' just went away. It's just another gun, and people like them." The 1994 law defined an assault weapon, listing several models of semi-automatic rifles and shotguns specifically, plus the features of any new models that would fall under this ban. The law listed various combinations of pistol grips, extended magazines, telescoping stocks, etc., that would provide the legal description of an "assault weapon." Detachable magazine size was limited to 10 rounds. "As tough as the law sounded, the ban was laughably easy to evade in practice," wrote Paul M. Barrett wrote in his 2012 book Glock: The Rise of America's Gun. Yes, manufacturers had to adjust the cosmetics of some guns to avoid the ban. But no matter how hard anyone tries, there's no simple definition for an assault rifle. "The true definition of an assault gun lies in how you use it," Drager said. "If you look at the Old West, they did a heck of a lot of damage with a double-barreled shotgun. Any firearm can be used as an assault weapon in a broad definition of it." It's important to remember that most of today's deer-hunting rifles are adaptations of firearms U.S. servicemen first used in various wars. One of the most popular pistols in America now remains a version of the Colt 1911 designed by John Browning in 1907. (The "1911" refers to the year when the U.S. military officially adopted it prior to World War I.) As for that proposed magazine restriction of 10 rounds, it sounded like a good idea to some when it was included in the 1994 federal law. But all it accomplished was the creation of another niche in the gun market. You could hear history repeating itself simply by standing in line to check in at a Las Vegas hotel Sunday. In a conversion with someone from a high-capacity magazine manufacturer, she told of sending 100,000 clips to a particular tradeshow recently and leaving with unfulfilled orders for another 245,000. Simply the hint of firearms restrictions after the Newtown, Conn., school tragedy sent America's gun-owners reaching deep into their wallets. The magazine restriction is undoubtedly well-intentioned, based on recent shooting tragedies involving 30-round magazines. But its effects are limited. "What's the difference in that and someone that can drop out one 10-round magazine and put in another and another," Drager said. "Or four eights or whatever? You're still talking about milliseconds." New York Gov. Andrew Cuomo signed a new gun law Wednesday that limits magazine capacity to seven, creating yet another niche in the market and accelerating demand for the higher capacity clips, where still available. The 1994 ban had a 10-year life, long enough for its effects to have been studied in detail by various entities. Most of the research came to the same conclusion as a 2004 U.S. Department of Justice report, which stated that rifles in general, including "assault weapons" are rarely used in gun crimes. In face of the recent horrors in Newtown and at the Colorado movie theatre, it's only basic human values that produce an effort to do something – anything – to prevent future tragedies like this. And maybe, with a more detailed examination of the wide-ranging proposals made by the President, some of the good intentions will produce results. "We're putting our emphasis on the wrong things," said Drager, again, a day prior to Wednesday's specifics. "It's only an illusion of accomplishment. People that shouldn't own guns shouldn't be allowed to. Those laws are already on the books. "If (a gun restriction) has any effect, it will make the market even stronger." Exactly. Those market forces are already in display. The industry will survive and adapt, like any strong one in a rapidly changing world. The gun industry's ultimate reply to the President will probably be "thank you," rather than the opposite. But nobody was saying anything like that here Wednesday. Share This Story Related Shooting : Posted 01-16-2013 NSSF Statement on Proposed Gun Measures View Hunting : Posted 01-15-2013 Companies Reveal Products to Conceal View Hunting : Posted 01-15-2013 Did Your Dog Bark Today? View Shooting : Posted 01-14-2013 'Media Day in Baghdad' View Hunting : Posted 01-13-2013 Making A (Deer) Stand View Comments Participating Sponsors


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100% saw instant fullness.
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'In Love' is a deep magenta shade.


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July 26, 1919 - August 23, 2013
Bethel, NY
Robert "Bob" Rossal, a lifelong resident of Bethel, NY, passed away on Friday, August 23, 2013 at his residence surrounded by his loving family. He was 94 years of age.
Bob was the son of the late Robert J. and Margaretha Boucher Rossal, he was born on July 26, 1919 in Bethel, NY. He was a retired self-employed dairy farmer and truck driver. He was a 74 year member of the White Lake Fire Dept. and a member of the Dairymen's League.
Survivors include his wife of 70 years, Hilda Bruning Rossal at home; son, Jon R. Rossal at home; stepson, Robert Bruning and his wife, Karen of Bainbridge, NY; daughters: Arlene Hillriegel and her husband, Robert of Fremont Center, NY and Jane M. Wall and her husband, James of Cochecton, NY; 12 grandchildren; 28 great grandchildren; 32 great great grandchildren and several nieces and nephews. He was predeceased by two sisters: Maye Menhesin and Olga Konopaf.
Visiting hours will be held from 4 to 8 p.m., today, Sunday, August 25th at Ramsay's Funeral Home, 3534 State Rt. 55, Kauneonga Lake, NY 12749. White Lake Fire Dept. will hold Firematic services at 7 p.m. Funeral services will be 2 p.m. on Monday, August 26th at Ramsay's Funeral Home; Rev. Paul Hagedorn will officiate. Burial will be at Evergreen Cemetery, Bethel, NY.
Arrangements are by Ramsay's Funeral Homes Inc., Kauneonga Lake, NY; to send an online condolence, please visit www.ramsaysfuneralhome.com.


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Kaylaa Suicide — Suicide Girl of the Day
Kaylaa is an exotic dancer, photographer, and Suicide Girl from Paris, France. As in, we see Paris, we see France...Sorry.
NAME: Kaylaa Suicide
LOCATION: France
HOMETOWN: Paris
OCCUPATION: Exotic dancer and photographer
CIGARETTES: Nope
ALCOHOL: Occasionally
MY IDEA OF A GOOD TIME: Bars, afterparties, home by dawn.
MY PIGEONHOLES: Hardcore, tattooed, piercing, geek
MAKES ME HAPPY: My girlfriend
HOBBIES: Photography, tattoos, voyages.


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Paper Cutouts Have Brutal Power in Noémie Goudal's Paris Solo Show
Inspired by her travels, photographer and installation artist Noemie Goudal creates images that occupy a space between surrealistic fantasy and modern life. Having once written stories to inspire her photographs, Goudal constructs large-scale installations in abandoned and obsolete structures in locations around the world, such as brutalist buildings and dilapidated barns, which she then photographs before enlarging them into life-size prints. She uses traditional chromogenic Lambda printing, adding a nostalgic element to compositions that are at once stark, romantic, and uncanny.
French, b. 1984, Paris, France, based in London, United Kingdom
Paper Cutouts Have Brutal Power in Noémie Goudal's Paris Solo Show
The Imagined Architectural Observatories of Noemie Goudal
Artsy Editorial


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Investigating the Visual Cortex
Students in Gina Turrigiano's lab explore how the brain balances excitation and inhibition
Gina Turrigiano has always been curious about the natural world. "I remember reading a book called Never Cry Wolf when I was 9," she recalls. "I decided that was it. I wanted to be a biologist."
Turrigiano has trained her focus on the synapses between neurons in the visual cortex. With a team that includes undergraduates, her lab investigates homeostatic plasticity—the brain's complex balancing act between the excitation and inhibition of neurons. The Marder Lab at Brandeis, where Turrigiano did her post-doc, as well as the Nelson and Griffith labs also work on homeostatic plasticity. This network of overlapping and specialized investigation allows for the collaborations that make Brandeis a hub for cutting-edge research and outstanding opportunities for undergraduate involvement. "If you're going to be a scientist, you have to see where the thread is leading you," says Turrigiano, "that takes an ability to think critically, to synthesize, to be creative. Brandeis does a phenomenal job of introducing undergraduates to this whole process."
Turrigiano's contributions to understanding how synapses between neurons modify signal strength in response to experience have been recognized with a MacArthur "Genius Grant," an NIH Director's Pioneer Award and the Nakasone Award, among many other honors.


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Posted 7 years ago
(310 items)
I would like to share this token I dug with a metal detector several years ago in Orange Grove, Texas.
As you can see, it is a $10.00 trade token from the Ingle System, and is a little larger than a quarter.
Here is what a Token Collectors group told me.
"Ingle was a company that manufactured tokens, and sold change dispensers to merchants. You would order 100 each of various denominations (or other quantities), load them into the dispenser, and press a lever to give out the correct change. The tokens are dated with patent dates, and "Ingle System".
I understand they came in denominations up to $100.00.


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Are you the new kid in town? Come to a New in Town Meetup to mingle with other new and not-so-new locals. Find fun things to do, cool places to go and a bunch of friends to come with you!
New In Town Meetups are just getting started. You can start your own or check out related topics


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It's hard enough having a serious condition like cancer or kidney failure. It's even worse, some might think, when your health insurer says you have to buy your medicine from the pharmacy of its choice — or pay the full amount for expensive life-saving drugs elsewhere.
Yet that's precisely what Anthem Blue Cross is telling people who require so-called specialty medicines, which are used for complex conditions and can cost thousands of dollars a month.
As of Jan. 1, the insurer says, such people can either buy their drugs from the mail-order pharmacy CuraScript or pay full fare at a retail drugstore.
"Using a retail pharmacy will be considered going out-of-network," Anthem says in a letter being sent to many users of specialty meds. "And your plan doesn't have coverage for that. So you'll have to pay the full price of the drug."


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Keep up to date with what's happening from KLRC and in your community with KLRC's Event Calendar! If you're organizing a community event, submit it to be included!
Prayer is an essential part of what we do here at KLRC, because we believe that God plays an integral role in both our station, and the daily lives of our listeners.
KLRC needs YOU! Our team of volunteers helps major events like Sharathon, concerts, and more go off without a hitch. We'd love to have you as a part of the team!
KLRC loves teaming up with local area non-profits to support the great work they're doing in our community! And each month, we choose one to highlight!
There are a lot of ministries and non-profits doing great work in our area, and we want to connect you to them! Check out our Non-Profit Database to get started.


text:
Achieve with us.®
Home ... Who We Are ... Position Statements ... Quality of Life
The primary goal for all persons with intellectual and/or related developmental disabilities1 is to enjoy and maintain a good quality of life.
People with intellectual and/or developmental disabilities must be able to live the lives they choose and have a good quality of life.
1 "People with intellectual and/or developmental disabilities" refers to those defined by AAIDD classification and DSM IV. In everyday language they are frequently referred to as people with cognitive, intellectual and/or developmental disabilities although the professional and legal definitions of those terms both include others and exclude some defined by DSM IV.
The Arc, 1825 K Street, NW, Suite 1200, Washington, DC 20006(800) 433-5255, ©2018 All rights reserved.
View Larger Map
Want to donate physical items? Contact a local chapter.


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2014 Fantasy Football PPR Rankings
It is never too early to start planning ahead for the 2014 season. For the majority of fantasy football players, the season ended about a month ago so next season could not come fast enough.
If we learned anything in 2013, it is that Peyton Manning, Drew Brees, Jamaal Charles, LeSean McCoy, and Calvin Johnson are money. These are the players you want to take in the first round of any league, regardless of the PPR status. These are the no doubters. The rest fill in the remainder of the draft board.
So, to make things go a lot smoother, we will take a look at the top players as we head into the 2014 season, of course we have not accounted for trades, free agency, injuries, or anything that might change their status so please keep that in mind when checking out this list.
Top 50 for 2014 Fantasy Football PPR
1. Jamaal Charles
2. LeSean McCoy
3. Peyton Manning
4. Adrian Peterson
5. Calvin Johnson
6. Drew Brees
7. Marshawn Lynch
8. Matt Forte
9. A.J. Green
10. Alfred Morris
11. Demaryius Thomas
12. Aaron Rodgers
13. CJ Spiller
14. Jimmy Graham
15. Dez Bryant
16. Arian Foster
17. Cam Newton
18. Brandon Marshall
19. Rob Gronkowski
20. Eddie Lacy
21. Josh Gordon
22. Julio Jones
23. Victor Cruz
24. Reggie Bush
25. Shane Vereen
26. Matthew Stafford
27. Randall Cobb
28. Antonio Brown
29. Zac Stacy
30. Alshon Jeffrey
31. DeSean Jackson
32. Maurice Jones Drew
33. Doug Martin
34. LeVeon Bell
35. Knowshon Moreno
36. Roddy White
37. Ryan Matthews
38. Andre Johnson
39. Percy Harvin
40. DeMarco Murray
41. Giovani Bernard
42. Frank Gore
43. Julius Thomas
44. Pierre Garcon
45. Jordy Nelson
46. Tom Brady
47. Andy Dalton
48. Eric Decker
49. Vernon Davis
50. Ray Rice


text:
Need A Home Mortgage? - Real Estate Services Classified Ads in West Sacramento
This is happening too much in today?s real estate market. Here?s what happens.
You find a property you want to buy. You get a great price and you know you can make money on this one IF you can get a loan. So you go to your bank but they say no.
The bank says you don?t make enough money or you own too many properties. Maybe they don?t understand your taxes. Maybe they?re just too scared to lend money!
Sound familiar? We can help solve this problem.
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Turned down by your bank?
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Call today at (707) 523-2900 or e-mail us at firstname.lastname@example.com
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An Equal Housing Lender
Business Spotlight
Small Business Funding


text:
I'm Proud to be An American
'Cuz at least I know I'm free
And I won't forget the men who died
To Give that Right to me
Etc. and so forth. Like this:
This is usually sung by someone whose clothing was made from an American Flag.
Country Singer or 90's Tae Kwon Do Instructor? We'll never know
Because the best way to honor the losses and sacrifices in the aforementioned song is apparently to cover your nipples with the flag that represents them.
And here's the thing. I'm not saying you shouldn't be "proud" to be an American. I'm just saying that maybe we should examine the reasons why we'r proud. That pride can be used to blanket over some really, really, terrible things.
And with the recent gun debates, personally speaking, I'm really starting to wonder if our actions as a nation stand up to our ideals. So let's chat.
Regardless of which side of the donkey-elephant thing you fall on, what does America stand for?
Like half of you just heard a Powerchord in your head
What's put right out there in the beginning? How did the damn thing start? Let's look:
We hold these truths to be self-evident, that all men are created equal, that they are endowed by their Creator with certain unalienable Rights, that among these are Life, Liberty and the pursuit of Happiness.
This is the document that said "We Are A Nation." And starts with "All Men Are Created Equal." This was written by a bunch of slave-owning white men. "All Men Are Created Equal and should be able to pursue Liberty except Black People, obviously, because COME ON," didn't sound nearly as eternal, I guess.
This is not a new argument. It's one of those things we like to just kind of shrug at, and continue using money with their faces on it. Because at the end of the day, it's really awkward that the dude one our most common form of currency owned people.
"I cannot tell a lie. And the truth is that slaves make things SUUUPER easy." Even the link above stresses how he freed all 300 of his slaves upon death. Like that's supposed to make up for it, or something. Yerg.
I care less about black people than George Bush, Kanye. Even. Less.
And in terms of civil rights, we're not exactly up there with the civilized world. In fact, for all that Daniel Day Lewis fixed Slavery and all that, we kinda did it last. Like after Moldova. And Uruguay.
Like, within 70 years of freaking Afganistan, which is known as "the asshole of the earth" by the people that LIVE there.
( I had an image here. I'm not gonna use it)
Pursuit of Life, Liberty and Happiness. If you have money. And are white, in America. Although whoever the Bourgeois Class has been throughout history have always done this. I wrote another relatively unpopular thing about that. But in America, it's been white men, specifically.
Okay, so women's suffrage.
We were right on par with Albania. Albania's chief national export is Sadness.
Black people can vote:
THE FUCKING 1960's.
My father was born in 1956.
I....I just....
(no wonder it's always white guys singing this)
(no wonder it's always white guys singing this)
Do we even really need to bring up gay marriage?
So again. Can we really say we stand for Liberty? Freedom? Equality? Look at our history. The whole goddamn thing, compared to the rest of the world. Because what we "stand for" has to be compared to something, right?
I know so many white people who felt proud of the fact we as a nation elected a black person to the presidency. I'm not saying this wasn't a good thing. The other alternatives were atrocious. He's done a pretty good job at fixing what the last guy did.
The point is WE SHOULDN'T BE FUCKING PROUD OF THAT. We should be ashamed it took so long.
"We all did this together. Equally. Sorry about the whole "spraying you with fire hoses thing. We cool, right?"
FUCKING FREEDOM? LIBERTY? COME ON. We're being beat out. We're not just losing the competition for math scores, America. We've been handily beaten on civil rights throughout history.
Gay people can't get married. BUT, but, but but but, you know what inalienable right everyone has? Everybody? Here's a list of what it's not:
1. Health Care
2. Marriage
3. Equal Pay
4. Marifuckingjuana.
No, but, we ALL CAN HAVE THESE:
This does less harm then two men living together
Can't take away our precious guns. Even the good guys aren't trying to get rid of them.
After Sandy Hook. And Columbine. And West Virginia.
It's more acceptable in our culture to believe that you HAVE to have guns. That ONLY GUNS are keeping the government from busting down your door and making you....uh, be oppressed.
THAT's an acceptable, agreed upon, ENDORSED viewpoint. It's not even worth arguing weather we should all have murder at the twitch of a finger lying around.
Thank god for you, sir. For protecting our rights.
Never mind that no such government coup has taken place in any other country that's ever banned guns, like Great Britain or Japan.
It's never. Even. Questioned.
This is the face of winning, apparently.
But can't get Gay Married. Can't.
Freedom. Liberty. Pursuit of Happiness.
It's not that I'm not proud to be an American because we have fucked up values. Lots of places do. It happens.
Seems like a healthy representation of sexuality.
It's that we're not honest about it.
We talk about Liberty, and Equality, and Freedom, and we just don't do it. Everyone, literally everyone else beats us to it, throughout history.
At every turn.
There's really one place we've excelled, historically speaking.
This is ours now.
And really, that's it. We shot 3 white guys to the moon. THAT'S. IT. We've been beaten at every other turn.
So I guess I have trouble being proud lately because we're in the end, as Americans, as a country, just not that special. We're not ahead of our times, we're not at the forefront of human rights, we're not even honest about what we're about.
And you know what? If the Amercan Flag looked a little less like this:
I have literally never seen a horse.
And just had THIS on it:
Maybe I could feel a little better.
2 probable internet responses:
1) But it's getting better! That's why this country's great! It can change!
Yeah, so can everywhere else, and faster than us on all counts. We're in no way special.
2) If you don't like it, leave, hippy sodomite!
Yeah, fuck off. Take your basement full of guns, secede, and get shot by cops my tax dollars paid for by bullets my tax dollars paid for.
Freedom of speech is number one. To take your guns, shove them up your ass, and get the fuck out of my country.


text:
Crab with Spinach & Black Bean Quesadilla
|Seafood, Beans
|Day Part
|Lunch, Snack, Dinner
|Menu Part
|Cuisine Type
A scrumptious and spicy quesadilla is filled with crab, spinach, cheese and black beans. Grilled until the cheese begins to melt and garnished with sour cream this makes a great appetizer.
8 oz. crab meat
8 large flour tortillas
1 4.5 oz. can green chilies
2 tbsp. cilantro, finely chopped
2 green onions, chopped
1 can black beans
8 oz. shredded Monterey Jack Cheese
1 10 oz. box of frozen chopped spinach, thawed
Cumin, to taste
Salt and pepper, to taste
Chili powder, to taste
Sour Cream, for garnish
1. Heat a large non-stick skillet over medium-high heat. Lightly oil skillet. Place a tortilla in skillet. Sprinkle some cheese evenly over top of the tortilla, followed by some chilies, crab meat, green onions, spinach, and black beans.
2. Sprinkle cumin, cilantro, chili powder, salt, and pepper to taste. Allow to cook for 2-3 minutes or until tortilla browns and cheese begins to melt.
3. Carefully fold tortilla in half and turn over and cook second side until lightly browned and cheese is thoroughly melted.
4. Garnish with sour cream. Repeat for all tortillas.


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Help Docs
Manuals for our various commands
Welcome to RuneScript's command documentation center. RuneScript is constantly expanding and coming out with new features. Each command is fully documented with all possible command syntax variations and detailed examples of the command in action. Each page also contains a brief overview of the command and information on how to change its settings. Remember you can also access quick help directly from the bot by using the !help command.
Lists of the exp values and levels currently used by RuneScript are available on the skills page. These are completely free to use, but they may contain the occasional error. If you stumble upon one please do not hesistate to report it.
Recent Additions
- • Meg - Search through the database of Meg's questions
- • Old School RuneScape Clans - Look up a user's clan on RSClanBase
- • Clans - Look up a user's clan in the Official RuneScape Clan Database
Featured Article: Skill Planner
Built-In Skill Planner
!<skill>-plan allows you to see how much exp you would gain from doing something a certain amount of times.
Syntax: !<skill>-plan [RSN] <number> <item>
-RuneScript- *** [ AGILITY PLAN ]: [Cowman 133] Original Level: 67 (548,055exp) | Exp gain of 170 Gnome Course: 14,705exp (86.5ea) | Final Level: 67 (562,760exp) | Exp needed for 68: 42,272exp (489 Gnome Course)
<Cowman_133> !fletch-plan MysticTiger 500 yew shieldbow
-RuneScript- *** [ FLETCHING PLAN ]: [Mystictiger] Original Level: 87 (4,076,460exp) | Exp gain of 500 yew shieldbow: 75,000exp (150ea) | Final Level: 87 (4,151,460exp) | Exp needed for 88: 234,316exp (1,563 yew shieldbow)
The Help Docs are maintained by Cowman_133


text:
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Dawning Light
Jenica Halliwell moves to Forks, Washington to fix one specific problem. However, as so often happens in Forks, one problem quickly multiplies into millions.
1. Chapter 1
Rating 4.5/5 Word Count 2372 Review this Chapter
My sister dropped me off at the door to the main building. Odd I know, I'm probably one of the few people who don't drive to school. Wouldn't you know it was raining, that's all it ever does in Forks I've come to find. Sunlight is a foreign concept. "I don't think we're in California anymore, Toto," I murmured to myself. I touched my sunglasses hesitantly, it was only out of habit that I had slipped them on that morning before walking out the door. If I sound a little down it's because I am, don't get me wrong I would do anything for a charge, but honestly rain was not made for romance.
The lady at the desk smiled, I gave her my name and told her I was the new student.
"Jenica Halliwell?" That's me, new student, sister, daughter of a charmed one, psychic, and cupid.
That's right you heard it, cupid. It's surprising to most people because when they hear the word cupid they don't exactly think of an eighteen year old girl from San Francisco. People usually conjure up ideas of babies in dippers with arrows, honestly Hallmark has ruined any respectability that my job may have once had. Not that I regret being a cupid—well ok half cupid, it has its perks like being able to look into peoples' hearts and see what they truly believe.
What makes that job unbearable at times is that I deal a lot with demons. As it turns out I'm not just the regular help-the-innocent-find-love kinda of cupid. No, I'm a Keeper of Keys, basically I get to look after demons who have proven themselves capable of human love. I get to open their hearts. That's why I'm here in Forks, something has gone terribly wrong with one of my customers, a certain Mr. Edward Cullen.
I nodded "That's me, but please call me Jen." Eh I hate Jenica my mother calls me that when I'm in trouble, blah.
"Alright, Jen here is your schedule," she handed me a slip of paper, "and you will need to get this paper signed," she said handing me yet another sheet, "by all of your teachers and return it at the end of the day." She smiled again, and handed me the slip of paper. "You seem to be transferring here a little late in the school year."
"Well my family will be here over the summer and my dad thought it would be an easier transition if I came early, to uh—make friends." She nodded as if she understood, which she probably didn't, and wished me good luck on my first day of school.
I wandered around the buildings that made up Forks High School. Weak rays of light streamed in through the heavy rain clouds. I stared down at my schedule, none of the courses were as hard as they could have been. I had the usual calculus, english, and chemistry, but I wasn't as scared of them as the assignments I would have to make up from Magic School. I walked into a building labeled 3 which housed my first class of the day: French.
The class I thought was about half full, but it turned out that classes are a lot smaller in Forks then they are in California. I took a seat in the second row next to a girl who looked that she was probably six feet if she stood up.
"Hi I'm Angela," She introduced herself.
"Jen," I replied feeling a little out of my element. While being the daughter of a Charmed One has its drawbacks the nice thing is that about half of that magical world knows who you are, and you don't have to go through the awkwardness of introductions. "Nice to meet you."
Any further conversation became impossible because the bell rang and the teacher began to drone on about indirect objects and their placement in the passe compose. My morning classes passed (surprisingly) in a whirl of information it was as if someone had cast a spell to speed up time. Before I knew it I was standing in line for lunch. I was wondering exactly where I was going to sit, when Angela invited me to sit with her "friends" and I use that word loosely, I've met nicer piranhas.
Lauren was evil, pure and simple, mental note to find her cupid and make sure she doesn't find love for like the next twenty years. Jessica is slightly nicer than her evil twin Lauren but still demonic. Tyler is cute and has possibilities but I'm taken. Ben is really nice, definitely not a piranha, and you can tell how much he loves Angela. Mike is just trying way too hard, but according to his file he's in kind of in a messy spot. However, he's at least nice to me and tries have a conversation.
"So you're from California? I lived there until I was ten."
"Cool," I replied, he was a nice guy after all, "Where about in California?"
"Up—well technically down from here, in Northern California. Where'd you live?"
"San Francisco."
"So why'd you move here?"
"Parents, why else, my dad got a new job and my mom's finishing up some stuff at home and then she'll come to," I said, it was all a lie, a perfect fabrication. My dad was here to make sure I didn't screw up, he's a full cupid although his powers aren't as extensive as mine. My mother is staying home because she has faith in me or something like that.
"Yeah." He asked a lot of random things like was I seeing anyone (Yes I am, sorry Mike, memo to self find Mike a girl) do I have siblings (Yes one older, one younger, oh the joys of being the middle child) where's your younger sister (Jess, my sister, attends boarding school) And the older one (Just finished a semester studying culinary arts in Italy) did you walk to school (No my sister dropped me off) What (Julie, my older sister, is here she dropped me off) my favorite color (Random? Anyway it's green.) Did I like the beach (Duh…) and where was I staying (Umm…a house on Magnolia, I think I am really bad about remembering street names)
"Really," he said after he found out my address, "that's the same street Bella lives on." Coincidence, right I let you all think that.
"Bella?" I asked, while I know who she is, she became my charge after the whole catastrophe that is Edward's love life, we haven't formerly been introduced. He pointed to a girl at the end of the table who was chatting with the black haired girl seated across from her. Bella looked over and I smiled and she smiled back, so first grade but whatever. I watched Edward stiffen, probably running into my empathy blocks right about now. The blocks don't block out my entire mind, but for him I'm sure he's wondering what's going on right about now.
"Mike, I think you've poked and prodded into Jen's life enough," Angela cut him off before he could continue his game of twenty questions. Mike looked sheepish and murmured, "Sorry."
"It's ok," I responded. I have to hand it to Mike he doesn't let anything bother him for long, he soon struck up a conversation with Lauren and forgot all about me.
"Thanks," I said to Angela, I was liking her more and more. "I thought his next question was going to be animal, vegetable, mineral?" She laughed.
"No problem, Mike sometimes gets carried away. You should have seen the interrogation that he gave Katie when she showed up."
"Katie?" Oh please don't tell me Katie's been here, well at least now I know who was inspiring major heartaches. You see, Katie and I have been mortal enemies since she decided to go all evil and abandon her charges. Katie was once a cupid, a special one like myself, a long time ago, but she fell in with the wrong crowd and changed sides so to speak. Now instead of hooking up love, she destroys it. Especially when I happened to be the one who made the connection in the first place.
"She moved here in the fall, but she recently moved away." Of course, that's her usual MO. Move in, break up, and get the hell out of dodge.
I nodded and finished my pudding, this was a stunning new development. "At the risk of sounding like a new girl, who are the people at the end of the table? Or do we not talk to them much?"
"Oh their cool, they just keep to themselves sometimes," She answered, "Alice is the one with black hair, she's in our French class, and then Bella you already know sort of, she's fun though well…anyway the guy on the end is Edward and," she added quietly, "he and Bella are dating."
I nodded, well at least things seem to be getting back on track. The bell rang, no more time for a walk down memory lane.
"Where are you going next?"
"Ugh, chemistry." As if I needed any help with handling chemistry, well ok maybe I need a little help handling that type of chemistry.
"Oh ok then come on, we can go together." Have I mentioned how much I like this girl? Note to self ask around for Angie's cupid and make sure that they give her an extra boost, and other note to self dispose of her other "friends."
The rest of the day was a blur of chemistry, PE (which I don't recommend taking, physical education is just—well there's no real way to describe what I feel towards PE), and calculus (whoever said math was a necessity was seriously disturbed).
Julie was waiting for me when I walked out of the main building after handing in my schedule to the very perky assistant. I nearly walked past her, I was too busy texting Ari to notice her.
"Hello," she called out, "earth to Jen."
"What." My head snapped up, "Hey Jules."
"Can you stop texting your friend for a minute and notice I'm here to pick you up? Loser."
"Idiot." I retorted. Don't be fooled by our interesting choice of pet names truly they are terms of endearment. Besides we love each other…most of the time. Julie is my older sister and protector of sorts, she's the one who makes sure I don't get killed while I unlock demonic hearts.
Julie shot past Edward Cullen, who was standing by his car looking at me. "Have you talked to him yet?"
"Absolutely not," I replied, talking about anything with Mr. Cullen would give me a ton of unneeded stress. Besides it's not like you can walk up to someone and say 'So I know you're a vampire and surprise I'm your cupid' that's just silly, not to mention stupid. "Honestly why would I talk to him in public."
"There's a saying, Loser, that you can catch more flies with honey then with vinegar. I don't mean that you should go and divulge that master plan you've been keeping to yourself, but a simple hello/goodbye might be nice."
"First off, I'm not catching flies, I'm talking to vampires. They are not insects, therefore vinegar works much better, they always want more of what they can't have. And as for my master plan it's still in the works, but I'll keep you posted."
"Thanks, you know I like to have a little time to get things together, but I understand you like to fly by the seat of your pants. It just doesn't work out so well for those of us who are responsible for making sure your pretty face doesn't end up as the next demonic target."
I looked around my room one last time, everything was set for those pesky nightly visitors. Waking charms, courtesy of my sister, she's great with coming up with spells at the drop of a hat, crystals, the whole ten yards. One quick scry told me that yes he was defiantly in the vicinity of my house.
My dad walked in, he still isn't completely sure that I'm alright, I mean you let your charges emotions take control of you for a few days, alright months, and suddenly there's no trust between us.
"Are you sure you have everything, honey?" my dad asked, he can't help but worry about me, and really in my line of work what parent in their right mind wouldn't worry. Though I wish he would trust me more. The Cullen's, however, are my first big case, and technically though I've been overseeing it from the beginning, I'm rather young for a Keeper. Hence, the tucking into bed routine, which he hasn't done since I was like four and finally figured out the boogieman was vanquished before I was born.
"Yeah dad, I have a waking charm on the window and the crystals are all set on the floor, besides Julie is just a shout away if I need her."
"Alright." He kissed me on my forehead and left my room.
I pulled the blanket up over my head and reached out a hand to flip off the light. I curled my hands around the final crystal and drifted off into the peaceful realms of sleep. I awoke in the early morning hours; well good to know that at least the waking spell was working. Now if this next part would just go as I planned it. I dropped the crystal on the floor so that he wouldn't be able to use his super sonic vampire powers to escape before I could talk to him. The plan worked surprisingly, and now I have one caged and outraged vampire trapped in my room. Wow, what girl doesn't want to wake up to that every morning? One in a million let me tell you."Good," I glanced at the clock, "morning Edward, we need to have a heart to heart chat."
- Author
- EbonyCircles
- Rating
1 2 3 4 5
- Reviews
- 18
- Words
- 6075
- Views
- 1
- Published
- 29 Jul 07
- Updated
- 26 Jan 08
- Status
- In Progress


text:
MIAMI (AP) — A juror says she feels like she let Trayvon Martin's parents down by agreeing to acquit George Zimmerman in the teen's fatal shooting.
In a taped interview aired Friday on ABC's "Good Morning America," juror B29 said jurors couldn't find enough evidence under Florida law to convict the neighborhood watch volunteer in the February 2012 death.
The juror agreed to let ABC show her face and use her first name, Maddy. She says she "fought to the end" but stands by the verdict returned earlier this month by the six-member, all-woman jury.
The 36-year-old Puerto Rican mother says the decision was difficult.
But the juror said she wants Martin's mother to know that her son will always be in her heart.


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Difference between revisions of "20.109(S13):DNA cloning (Day4)"
(→Part 2: Purify 16S PCR band from gel)
(→For next time)
|Line 205:
|Line 205:
==For next time==
==For next time==
==Reagent list==
==Reagent list==
Revision as of 18:03, 13 February 2013
- 1 Introduction
- 2 Protocols
- 2.1 Part 1: Gel electrophoresis of PCR products
- 2.2 Part 2: OPTIONAL -- Purify 16S PCR band from gel
- 2.3 Part 3: Ligate purified product to vector arms
- 2.4 Part 4: Prepare tubes for liquid O/N cultures
- 2.5 Part 5: Transform ligated product into cloning strain
- 2.6 Part 5: Set up microsporidia PCR on this day also? Starting to lean towards Day 2 after all...
- 3 For next time
- 4 Reagent list
background on this cloning system -- vector arms and strain
You will melt the agarose gel bands, then isolate the DNA by using a silica (SiO2) column similar to the one you used last time. Salt concentration and pH effects, along with ethanol precipitation, will alternately allow for binding and eluting the DNA while washing away contaminants. The final elution here will be done in water, and these silica columns will collect any DNA between about 70 and 10,000 bp.save some of this for next time?
Part 1: Gel electrophoresis of PCR products
You will use a 1% agarose gel to run your three PCRs from last time, as well as a reference lane of molecular weight markers (also called a DNA ladder).
- Add 3 μL of loading dye to each of three eppendorf tubes. No need to change tips in between.
- Loading dye contains xylene cyanol as a tracking dye to follow the progress of the electrophoresis (so you don't run the smallest fragments off the end of your gel!) as well as glycerol to help the samples sink into the well.
- Now add 30 μL of each reaction from last time (your sample, your partner's sample, and the no template control) to individual eppendorf tubes and label them.
- If a reaction has less than 30 μL due to evaporation, take the maximum amount you can and write it down.
- Flick the eppendorf tubes to mix the contents, then quick spin them in the microfuge to bring the contents of the tubes to the bottom.
- Remember: to quick-spin, hold down the "short" button on your centrifuge for 3-5 seconds, then release.
- Load the gel according to the table below. Up to 4 groups will share each gel: 2 groups per lane.
- To load your samples, draw 30 μL into the tip of your P200. Lower the tip below the surface of the buffer and directly over the well. You risk puncturing the bottom of the well if you lower the tip too far into the well itself (puncturing well = bad!). Slowly expel your sample into the well. Do not release the pipet plunger until after you have removed the tip from the gel box (or you'll draw your sample back into the tip!).
- Once all the samples have been loaded, we will attach the gel box to the power supply and run at 110 V for 45 minutes.
- Need to check what voltage/time works well for a lower % gel; have been using higher % during pilots due to microsporidia work.
- Later you will be shown how to photograph your gel and excise the relevant bands of DNA. Begin by anticipating where you expect to see your sample band relative to the bands of the DNA ladder, described here.
- While the gel runs, weigh two eppendorfs and label each tube with its weight. By preparing these in advance, we won't encounter unnecessary delays during purification.
|Sample (30 μL)
|Sample (30 μL)
|DNA ladder (load 10 μL)
|DNA ladder (load 10 μL)
|Group 1, NTC
|Group 2, NTC
|Group 1, sample A
|Group 2, sample A
|Group 1, sample B
|Group 2, sample B
Part 2: OPTIONAL -- Purify 16S PCR band from gel
To purify your DNA from the agarose, you will use a kit from the Qiagen company. As we learned during the DNA extraction step on Day 2, reagents in such commercial kits can have uninformative names and their contents are in part proprietary.
- Estimate the volume of your gel slices by weighing them.
- The easiest way to do this is to pre-weigh an eppendorf tube for each slice, then weigh it again after adding the gel, and take the difference.
- What can you assume about the density of agarose and why?
- Add 3 volumes of QG for every 1 volume of agarose.
- The maximum advised volume is 550 μL. If you have a greater volume, continue for now, but first read step 6 to understand how to proceed later. Feel free to ask the teaching faculty for clarification.
- Incubate in the 50°C water bath for 10 minutes, until the agarose is completely dissolved. Every few minutes, you should remove your tubes from the 50°C heat and flick or vortex them for a few seconds to help dissolve the agarose.
- Add 1 volume — original gel volume, not current solution volume — of isopropanol to each eppendorf tube and pipet well to mix.
- Get two QIAquick columns and two collection tubes from the teaching faculty. Label the spin-columns (not the collection tubes!) with your sample IDs and then pipet the appropriate dissolved agarose mixture to the top of each one. Microfuge the column in the collection tube for 60 seconds at maximum speed (approx. 16,000 rcf). The maximum capacity of the QIAquick columns is 800 uL! If you have more than 800 uL in your mixture, you will need to repeat this step using the same column.
- Discard the flow-through in the sink and replace the spin-columns in their collection tubes. Add 500 μL of QG to the top of the column and spin as before.
- Discard the flow-through again, then add 750 μL of PE to the top of the column and incubate for 5 min at room temperature.
- Spin for 1 min as before.
- Discard the flow-through in a temporary waste container such as a 15 mL conical tube (PE contains ethanol) and replace the spin-columns in their collection tubes.
- Add nothing to the top but spin for 60 seconds more to dry the membrane.
- This step completely removes remaining ethanol that could interfere with future reactions.
- Trim the caps off of two new eppendorf tubes and prepare sticky labels (in your team color) for the top: write the date, your section day, and the sample ID. You may also want to label the side of each tube, so you don't lose track of which sample is which in the following step.
- Place the labeled spin-column in its matching trimmed eppendorf tube and add 30 μL of pH 7 water to the center of the membrane.
- Do not add regular distilled water, as this is at a lower pH, which will lower elution efficiency.
- Allow the columns to sit at room temperature for one minute and then spin as before. The material that collects in the bottom of the eppendorf tubes is your purified 16S DNA.
Part 3: Ligate purified product to vector arms
- Prepare a clearly labeled eppendorf tube for each cloning reaction.
- In the following order, combine 3 μL cloning buffer, 2 μL purified DNA, and 1 μL of vector mix.
- Incubate at room temperature for 5 min, and then move to ice if you are not yet ready to proceed with the next step. Meanwhile, obtain an aliquot of competent cells from the teaching faculty and let them slowly thaw on ice. Label the side of each tube (and also the top if you like).
- When both cells and reaction are ready, add 1 μL of the reaction to the cells. Do not pipet up-and-down more than once! Instead, gently mix the cells and DNA by twirling the tube in your fingers.
- Hold the tube near the top, so that the cells at the bottom stay cold.
- Incubate the mixture on ice for 20 min.
- Place the tubes in the 42 °C heat block for exactly 45 seconds, and transfer immediately immediately to ice for 2 min more.
- Add 250 μL of warm LB to each sample; do not pipet to mix.
- Place the tubes on the nutator in the 37 °C incubator, and rock them for about 1 hr.
- You may want to put a colored sticky label on each tube so you can quickly identify them later.
- Pre-warm 2 LB-Amp-Xgal plates in the incubator at least 20 min prior to using them in the next step. Also be sure to label the plates in advance with your sample IDs.
Add positive and/or negative control plates?
Part 4: Prepare tubes for liquid O/N cultures
You will make your teaching faculty very happy if you contribute to their preparatory work.
- Please label X large glass test tubes with your team color and sample ID, per sample.
- Mix Z mL LB with Z μL of ampicillin.
- Using a serological pipet, aliquot 2.5 mL of LB+Amp per tube. These will be used to set up liquid overnight cultures from your X colonies for next time.
Part 5: Transform ligated product into cloning strain
- Sometime during the hour incubation step, watch the teaching faculty demonstrate how to transform cells.
- Pick up an ethanol jar, spreader, lighter, and alcohol burner when you are almost ready to begin. Don't forget to wear your safety glasses!
- Plate 100 μL of each transformation mix onto an LB-Amp-Xgal plate. After dipping the glass spreader in the ethanol jar, you should pass it through the flame of the alcohol burner just long enough to ignite the ethanol. After letting the ethanol burn off, the spreader may still be very hot, and it is advisable to tap it gently on a portion of the agar plate without cells in order to equilibrate it with the agar (if it sizzles, it's way too hot). Once the plates are ready, wrap them together with one piece of colored tape and incubate them in the 37°C incubator overnight. One of the teaching faculty will remove them from the incubator and set up liquid cultures for you to use next time.
Part 5: Set up microsporidia PCR on this day also? Starting to lean towards Day 2 after all...
need to do microsporidia PCR on separate day because the Ta etc. is different --> need to find notes on where I planned to put that. possibly Day 2? primers should have arrived by then. or maybe wait until Day 4? don't want to have them balancing lab steps too soon.
- Begin by carefully labeling each PCR tube that you will use with the date, sample name, and a unique symbol and/or color for quick identification. Filling in the cap tab with your team color will usually suffice. Pre-chill the tubes on a cold block.
- You and your partner can now prepare and share a so-called "master mix," which contains every PCR ingredient except the template and the polymerase. Prepare enough for the number of reactions you need to run, plus an additional 10%. Feel free to use the table below for your calculations.
- When the master mix is not in use, keep it on ice.
- Combine 5 μL of template, 45 μL of master mix, and 1 μL of PfuUltra polymerase in a PCR tube. When everyone's reactions are ready, they will undergo the cycling conditions listed below.
- Add the master mix first, because the template alone may freeze. Then add template and polymerase, and finally (gently!) mix the reaction with a larger pipet.
|Amount for 1 reaction (μL)
|Amount for ? reactions + 10%
|PfuUltra buffer (10X stock)
|Primer mix [TO WHAT EXTENT PREPARED FOR THEM?]
|5 of each (Fx and Rx)
|10% BSA (100X stock)
|DNA template
|Temperature (° C)
|3 min
|1 min
|1.5 min
|2 min
|10 min
For next time
- Prepare the overview schematic of your experimental approach that will be included in the Introduction or (more likely) at the beginning of the Results.
- Recall that our collaboration and integrity guidelines state that your text and figures must be your own unless otherwise specified. Thus, you should all make your own schematic rather than modifying one from class slides or the wiki. Keep in mind that you are being evaluated not on advanced PowerPoint skills, but instead on showing that you understand the purpose and major steps of the experiment and can convey them.
- and
Reagent list
- Agarose gels
- Standard agarose prepared in TAE buffer at 1%
- With SYBR Safe stain (Invitrogen)
- used at manufacturer's recommended concentration, 10000-fold dilution
- Loading dye
- 0.25% xylene cyanol
- 30% glycerol
- RNase
- Gels made and run in 1X TAE buffer
- 40 mM Tris
- 20 mM Acetic Acid
- 1 mM EDTA, pH 8.3
- 100 bp DNA ladder from New England BioLabs
- Qiagen QIAquick gel extraction kit
- silica spin columns
- QG and PE buffers
- Isopropanol
- StrataClone Blunt PCR Cloning kit from Agilent
- Includes StrataClone SoloPack competent cells


text:
Ian Bell's highest score in a home Ashes series helped England emerge from another day mired in controversy with a match-winning opportunity at Trent Bridge.
Bell's unbeaten 95 perhaps did not deserve to be overshadowed by any more intrigue in a series only three days old, yet already jam-packed with incident.
But that will doubtless be the case after Aleem Dar failed to pick up an edge so blatant by Stuart Broad, off Ashton Agar, that it carried via a faint deflection off the wicketkeeper's gloves into the safe hands of Australia captain Michael Clarke at first slip.
The tourists had no recourse to DRS, having already used up two permitted reviews, so Broad survived on 37 and was still there at stumps when England reached 326 for six and a lead of 261.
They profited from Broad's good fortune, and judgment of what is acceptable when it comes walking or otherwise, as they also cashed in on the hard work of Bell, Alastair Cook (50) and Kevin Pietersen (64).
England were apparently seething on Thursday when they felt they were the wronged party after Jonathan Trott was lbw for a golden duck following what they felt was an unsatisfactory DRS process.
On Friday, they were the beneficiaries when an obvious edge went undetected - but more than that the concept of sportsmanship, in the rarefied atmosphere of cricket's oldest battle, appeared to be endangered.
Before the whys and wherefore of Broad's reprieve, when England were 297 for six, Cook and Pietersen's century stand and then Bell's skill had already combined to ensure the series' first dominant close-of-play position would belong to the hosts.
The last thing England needed was to allow any sense of perceived injustice over Trott's dismissal on Thursday - or indeed Agar's survival of a marginal stumping decision, both involving third umpire Marais Erasmus - to deflect them from their primary purpose.
It was to Cook and Pietersen's great credit that they did not, at least until each had a valuable 50 to his name.
The pair had first joined forces at 11 for two, and on a hot and hazy morning took their stand to 110 before Pietersen got a thick edge back on to his middle-stump as he tried to carve James Pattinson through the off-side.
Pietersen's mistake was a shock after he had become progressively assured, particularly with his impressive driving down the ground and through the off-side.
He counted two fours from false shots too, an inside-edge past fine-leg from Peter Siddle's attempted yorker and one off the outside of the bat past cover as he aimed to hit Mitchell Starc to leg. But Pietersen's 10th boundary, a cover-drive off Starc, was in more authentic mould and completed his 120-ball 50.
Cook stayed long enough, with his third-wicket partner gone, only to reach his first Test half-century at this venue before departing to the 165th ball he faced - a maiden wicket on debut for Agar, Australia's unlikely number 11 batting hero on Thursday.
Cook fell to a one-handed slip catch by Clarke, high to his left, when he closed the face and edged one that did not turn from Agar. Bell was therefore joined first by Jonny Bairstow and then Matt Prior for contrasting stands of 43 and then 44.
Bairstow batted against type, stifling any attacking instincts until he fell to Agar's left-arm spin - walking instantly when he was caught-behind pushing forward in defence. Prior then climbed into a series of trademark boundaries, chiefly through the off-side, but mistimed a pull at Siddle with the second new ball off the shoulder of the bat to midwicket.
Bell has not always been England's most reassuring presence, despite a fine record which has taken him past 6,000 Test runs - and on Friday he also had to defy discouraging statistics which previously gave him an average at home to Australia in the low 20s, including a top score of only 72, and mostly slim pickings too in Nottingham.
But his only scares came when DRS spared him after Kumar Dharmasena initially gave him out lbw to the miserly Shane Watson on 34, before 43 runs later he edged a half-chance past Brad Haddin off Siddle.
Among his 12 fours from 228 balls were a glorious cover-drive to get off the mark and then two memorably deft late cuts between slip and a fine gully in one over, all off Pattinson.
Clarke posted some characteristically eccentric fields to him, and especially Broad - but all to no avail on a day, unlike the previous one, when almost everything went England's way.
Bell shines on controversial day
Ian Bell's highest score in a home Ashes series helped England emerge from another day mired in controversy with a match-winning opportunity at Trent Bridge.


text:
Arguably the most important social media tool around is Twitter. For journalists – the ones that "get it" at least – it has meant a new network of contacts, shared information and friends.
News is increasingly broken by ordinary people with iPhone Twitter apps or normal office workers sat at their desks updating Twitter by web or Gtalk and not by dashing reporters in long macs, or even agency reporters in far-flung bureaux. It is the canary in the news coal mine.
So it's great and everybody loves it.
But where are the PRs? As Michael Cooper (@michaelcooper) rightly said a while back, "If newsdesks had Twitter at their disposal, the relationship between hacks and flacks could change dramatically".
He says: "From instant updates like 'Don't bother me. I'm on deadline!' through to 'Looking for urgent case study about….' journalists should be using Twitter as a tool to interact with PRs. If newsdesks are evolving into 24-hour bodies, maybe it's time for their journalists to move away from resources like ResponseSource to a more immediate communication tool."
Indeed. But more and more, on some national papers and forward-thinking regionals, journalists are getting it big time. Trinity Mirror seem to be leading the way in both Liverpool and Birmingham where journalists use Twitter to connect with people locally as well as to break and aggregate news. But are PR officers seizing the opportunity?
Though I know people who have, I've never been approached by a PR on Twitter and I would quite happily discuss story that way. Or what about sending press releases or statements out that? Downing Street does. Even the Mars Lander does.
And for PRs it could be a good career move – Todd at PR-Squared says he has hired people because they handled themselves well on Twitter.
So if you want me to write about your newspaper/website/magazine follow me and let's start talking. And if you are in public relations and already a prolific Tweeter, get in touch and prove me wrong.
UPDATE 07-08-08 – A Twitter friend tweets to say that tech PR people are 'all over it, unsurprisingly'. That would make sense, it's just not something I've seen myself.


text:
Wednesday, November 19, 2008
Pumpkin Roll
14 comments:
Oh, it's like your in my kitchen. I have nearly full can of pumpkin in my fridge just dying to be used for something better than the pumpkin & ricotta spread I've been making out of it.ReplyDelete
Not only fantastic, but so pretty!
Dana - I figured people have open cans of pumpkin in their fridge, since most pumpkin recipes except pie don't need a whole can. This is soo good. And impressive for guests!ReplyDelete
Lea - Do it. It's good. It's not super pumpkin-ey, though. I wish it were a little more pumpkin flavored, I might add some to the filling or a pumpkin white chocolate ganache next time!
Looks like a great alternative for thanksgiving!ReplyDelete
OMG... JILL!ReplyDelete
Gorgeous Photos, Girl!
And.. please.. if you think that pumpkin roll can survive a Phily to Seattle flight... do send some my way!
Ingrid - I don't have a recipe for white chocolate ganache, I was just thinking about next time!! And yes, all the pictures I post are mine unless stated otherwise!ReplyDelete
I saw that recipe on Libby's site but I wasn't convinced to make it until I saw your pictures, it looks so much more appetizing here! Nice work.ReplyDelete
PB Boy - I was really unimpressed with the pictures on their site as well, I'm glad you like mine!ReplyDelete
WHAT THE! GIVE ME! I LOVE PUMPKIN! GIVE ME PUMPKIN ROLL! AHHH!ReplyDelete
Hey Matty, come over sometime I'll bake you something delish. You and Cliff owe me a visit from oh, like two years ago?ReplyDelete


text:
Gonzalo wrote:The strenght of the young people.
Gonzalo wrote:As a noun, it is iuvenum for genitive plural. As an adjective, it is iuvenium.
Gonzalo wrote:Hi,
I have learnt it by usage and not by grammar rules but I found anyway in my Latin lexicon what I have referred to and, besides, I have found the next reference -I have just written "iuvenis" at Google:
http://en.wiktionary.org/wiki/iuvenis Take a look.
Boban wrote:But if you reffering to noun "iuvenis, is, m." then genitive plural is "iuvenium" because iuvenis, is, m. is parisyllaba with genitive -is, so belongs to III-i declination.
Boban wrote:Difference betwen III declension and III-i declension is only in genitive plural ending.


text:
Image: Blue Sea Slug Glaucus atlanticus and Porpita Porpita pacifica
Blue Sea Slug, Glaucus atlanticus, and Porpita Porpita pacifica.
- Photographer:
- Peter Parks
- Rights:
- © Image Quest 3-D
This brilliant Blue Sea Slug is beautifully adapted for life floating upside down in the sea and is often found with the beautiful blue jellyfish Porpita. Blue Sea Slugs feed almost exclusively on the tentacles of Bluebottles, Physalia species. Interestingly, the nematocysts (stinging cells) on these tentacles pass through the Blue Sea Slug intact. The slug can then use these stinging cells in its own defence.
Last Updated:
Tags sea slugs, molluscs, sea jellies, jellyfish, porpita, cnidarians, marine invertebrates,


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Lakeland University
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Mehraban Khodavandi
Professor of Education & Psychology
Miscellaneous Information
Mehraban Khodavandi, who joined Lakeland in 1981, earned his Ph.D. in educational administration from the University of Wisconsin-Madison in 1980.
Khodavandi has served as chair of the education division and is currently the director of Lakeland's Graduate Education Programs and the administrator of the Alternative Teacher Certification Program through Milwaukee Teacher Education Center. He is a two-time winner of Lakeland's Outstanding Professor of the Year award and a winner of the Underkofler Excellence in Teaching award, and a winner of the Alumni Association Outstanding Faculty Member award.
Khodavandi, who has one year of post-doctoral credit in the area of vocational and adult education, has been the driving force in Lakeland's undergraduate and graduate education programs.
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Loyalty Day Parade rolls through Standish
Participants in the 42nd annual Loyalty Day Parade made their way through Standish Saturday, April 27. Local veterans organizations, civic groups, business representatives and elected officials marched or rode by in the parade, which stretched from Airpark Drive east to the Standish Elementary School. The parade's grand marshal was Bob Sanch Sr., a lifetime resident of the area, a VFW member and business owner.
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Ormat Technologies, Inc. Announces a Non-Cash Pre-Tax Charge for Impairment to its North Brawley Geothermal Power Plant
Jan 23, 2013, 01:30 EST
RENO, Nev., Jan. 23, 2013 /PRNewswire/ -- Ormat Technologies, Inc. (NYSE: ORA) announced today that it will record a non-cash pre-tax charge of up to $230 million during the fourth quarter of 2012 for impairment to the company's North Brawley power plant located in Imperial County, California.
(Logo: http://photos.prnewswire.com/prnh/20040422/LATH066LOGO)
The North Brawley power plant was placed in service under its power purchase agreement with Southern California Edison (SCE) in 2010. However, the North Brawley geothermal field has been much more difficult to operate than other fields and the power plant has been unable to reach its design capacity of 50 MW. Instead, it has been operating at capacities between 20 MW and 33 MW. This generation level has been achieved following significant additional capital expenditures and higher than anticipated operating costs.
In order to improve the economics of the North Brawley power plant, the company approached SCE to discuss various contractual alternatives to the power purchase agreement and, in early 2012, reached a written understanding to engage in discussions with third parties about purchasing the power at better rates. However, in a letter dated January 14, 2013, SCE informed the company that it is no longer interested in pursuing alternatives to the current power purchase agreement, thus retracting its permission to the company to explore a replacement power purchase agreement with higher electricity prices.
As a result of SCE's notification and the resulting continuation of the current low price regime under the existing power purchase agreement, coupled with a further understanding of the cost and probability of success of additional well-field work which has been accumulated in the recent months, the company has concluded that it will not be economical to continue to invest the substantial capital required to increase the generating capacity of the North Brawley plant. Accordingly, the company has decided to operate the plant at the current capacity level of approximately 27 MW and refrain from additional capital investment to expand the capacity. The company expects to record a non-cash pre-tax charge for impairment to the North Brawley power plant in the fourth quarter of 2012, which is currently estimated to be in an amount of up to $230 million. The impairment charge will result in a material loss in the fourth quarter and for the full year 2012. The company does not expect that the impairment charge will affect its ability to meet the financial covenants under any of its corporate debt agreements or materially adversely affect its future operational results.
"Although we expect that the substantial reduction in operating expenses through 2012 will result in positive EBITDA for North Brawley going forward, the inability to replace the current power purchase agreement with a new agreement providing higher electricity prices, coupled with this facility's higher than typical operating expenses drove our impairment decision," said Ormat CEO Dita Bronicki. "Given these circumstances, additional investment to increase generation is not economically justified. However, we will continue to explore ways to improve North Brawley's future cash flows to deliver value for shareholders," added Mrs. Bronicki. "Where applicable, we have implemented operational lessons learned from the North Brawley experience in the development of new projects brought on-line in the last two years, thus improving our ability to develop successful and profitable projects," concluded Mrs. Bronicki.
Separately, the company notes that it has been advised by Ormat Industries Ltd., the company's parent entity, that the parent intends to record a pre-tax, non-cash charge in a currently estimated amount of between $30 million and $40 million during the fourth quarter of 2012 for impairment to the company's Jersey Valley power plant based on International Financial Reporting Standards (IFRS). The company has independently conducted its own impairment test for the Jersey Valley power plant in accordance with generally accepted accounting principles (GAAP), and has concluded, based on the results of its examination, that no impairment charge is required under GAAP with respect to the Jersey Valley power plant.
Conference Call Details
Ormat will host a conference call to discuss the matters included in this press release at 9 a.m. EST on Wednesday, January 23, 2013. To participate, please dial (888) 562-3356 (U.S. and Canada) or (973) 582-2700 (international). The call will be available as a live, listen-only webcast at www.ormat.com. The webcast can be accessed through the Webcast & Presentations in the Investor Relations section of Ormat's website. The webcast will be available approximately two hours after the conclusion of the live call. A replay will be available from 12 p.m. EST on January 23, 2013 until 11:59 p.m. EST on January 30, 2013 by dialing: (855) 859-2056 (U.S. and Canada) (404) 537-3406 (International) and enter the replay code: 92522007.
About Ormat Technologies
Ormat Technologies, Inc. is a leading geothermal company and the only vertically-integrated company in the geothermal and recovered energy power business. The company designs, develops, owns and operates geothermal and recovered energy-based power plants around the world. Additionally, the company designs, manufactures and sells geothermal and recovered energy power units and other power-generating equipment, and provides related services. The company has more than four decades of experience in the development of environmentally-sound power, primarily in geothermal and recovered-energy generation. Ormat products and systems are covered by 82 U.S. patents. Ormat has engineered and built power plants that it currently owns or has supplied to utilities and developers worldwide, totaling over 1,500 MW of gross capacity. Ormat's current generating portfolio includes the following geothermal and recovered energy-based power plants: in the United States - Brady, Brawley, Heber, Jersey Valley, Mammoth, McGinness Hills, Ormesa, Puna, Steamboat, Tuscarora, OREG 1, OREG 2, OREG 3, and OREG 4; in Guatemala - Zunil and Amatitlan; in Kenya – Olkaria III; and, in Nicaragua - Momotombo.
Ormat's Safe Harbor Statement
Information provided in this press release may contain statements relating to current expectations, estimates, forecasts and projections about future events that are "forward-looking statements" as defined in the Private Securities Litigation Reform Act of 1995. These forward-looking statements generally relate to Ormat's plans, objectives and expectations for future operations and are based upon its management's current estimates and projections of future results or trends. Actual future results may differ materially from those projected as a result of certain risks and uncertainties. For a discussion of such risks and uncertainties, see "Risk Factors" as described in Ormat Technologies, Inc.'s Annual Report on Form 10-K filed with the Securities and Exchange Commission on February 29, 2012 and Quarterly Report on Form 10-Q filed with the Securities and Exchange Commission on November 8, 2012. In addition, the amount of the impairment charge is a preliminary estimate and is subject to a third party valuation, and a final review of the company's management and review by Ormat's audit committee. The actual impairment charge and the final result may be different from the preliminary estimate presented herein.
These forward-looking statements are made only as of the date hereof, and we undertake no obligation to update or revise the forward-looking statements, whether as a result of new information, future events or otherwise.
Ormat Technologies Contact:
Investor Relations Contact:
Dita Bronicki
Todd Fromer/ Rob Fink
KCSA Strategic Communications
212-896-1215 (Todd) /212-896-1206 (Rob)
SOURCE Ormat Technologies, Inc.