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How do Hill and Golding present a warning to society?
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Transfer-Encoding: chunked Both writers utilise their respective novels effectively as warnings to society. Golding?s novel is written especially in an allegorical style, with hidden messages that can be interpreted to give his view on society. Hill was also known to be a critic of the British upper classes, and so it is no surprise that her major warnings to society revolve around this. The titles of both novels involve ?Kings? and ?Lords?, both of which roles have a large influence on society, and possibly, the authors are trying to warn the readers of the effects that these people in positions of power can have. Both writers warn the readers of the negative effects of the upper classes, as well as warning of the effects of the patriarchy, and the effects of isolation from civilisation. Both writers are very critical of the behaviour of the upper classes and British Empire in their novels, and warn society of these effects. Jack is said to have used a face ?mask? of ?red and white?. These colours could possibly symbolise the colours of the English flag and patriotism, as well as being symbolic of the British Empire. However, the fact that it is described as a ?mask? has tribal, or savage connotations, and Golding could possibly have been using this to portray how the British Empire and the upper classes use patriotism as a facade for their otherwise savage behaviour, and this patriotism is further shown by the exclamation ?the English are the best at everything?. ...read more.
The fact that the arrival of the boys impacts a ?scar? upon, the island, as well as creating ?decaying skull-like coconuts? further shows the message that Golding is trying to convey about masculinity. The use of the word ?scar? suggests a sort of permanent damage, and unsightly destruction, and the ?decaying skull-like coconuts? further portray this, as well as conveying messages of death and suffering brought by the arrival of the boys. The idea that the sow - the only female living thing on the island - is killed by the boys further showcases this oppression of femininity by the boys, and Golding?s use of language such as ?inch by inch? and ?eruption? holds graphically sexual undertones, further portrays the destructive oppression of the patriarchy. Golding possibly uses this negative portrayal of the behaviour of boys and men to warn society of the damaging effects of the patriarchy, and a feminist reading of the novel could argue that Golding is trying to bring to light to downfalls of the patriarchy.Hill reflects this in her novel using the portrayal of Mrs Kingshaw. She is seen to be the only prolific female character in the novel, and this in itself portrays the way in which she is isolated. She is said to ?shorten the hem of her skirt?, and this shows how she has no choice but to succumb to the sexual desires of Joseph Hooper, and the oppressive behaviour of masculinity, in the patriarchy which is especially present in the upper classes. ...read more.
The idea that ?all the Warings land had been sold off, piece by piece? furthermore shows how isolated the area of Warings is. This is conjunction with Hooper?s ?relentless persecution? causes Kingshaw to feel this ?extreme isolation?, and it is this isolation which causes Hooper to ?drop down on all fours? and ?slither?. The image of ?all fours? suggests that Hooper is behaving in an animalistic manner and inhumanly, and the image of ?slither? compares Hooper to a snake, known to be cunning and sly. Moreover, Hooper talks about how ?that?s what hunters do?, which shows his possibly violent aspirations. All this in conjunction results in Hooper being portrayed as savage and inhumane, brought about from the isolation of Warings from the rest of civilisation. Hill uses the Gothic style to portray the isolation of the upper classes from the rest of society, and uses this to explain the negative influences of their behaviour on society. She does this similarly to Golding, utilising isolation to convey her personal beliefs on mankind, and both writers warn of the possible effects of isolation from civilisation Overall, both writers present strong ideas in their novels, and utilise the negative events in either novel as a warning to the reader of what could possibly happen in the further. Golding uses Lord of the Flies as a prophetic, allegorical text, to convey his own ideas, while Hill writes in the Gothic style to convey her warnings to the reader. ...read more.
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Or maybe the question is, "Will they ever go away?"
Like many other financial institutions out there, we offer free Bill Payer and we used to have a checking account that rewarded members with a dividend if they consented to receive their statements and communications with us electronically. And yet we continue to have thousands of members that use paper checks, no matter what incentive we offer. I have yet to hear of a bank or credit union getting rid of paper checks altogether, but it appears that one particular mass retailer might be discouraging their customers from using paper checks. Or maybe it was a lapse in training?
A friend of mine experienced this and she blogged about it:
"I recently got back from a quick trip to the grocery store. I've been putting it off for over a week now. I find that now that I don't have my own income, I procrastinate doing things that involve payments. Grocery shopping is high on the list of things I would rather do with K… part because two kids, two parents… part because, then he can pay for the goods.
I'm used to using my debit card when I'm out. It's convenient and it takes the money directly out of my account. But, I only have a debit card for my account, not our joint account. That means that while I have been unemployed, I've had to write checks for groceries. UUUGH!
My dislike of the paper check is apparently shared by many. I've pulled out my check book numerous times these past several months and I hear moans and groans from those in line… as was the case tonight. It made me think about the time I went to Walmart when they just opened.
It had been opened for maybe a week. I had to get some necessities late at night (they are open 24 hours) and, of course, I had to write a check – I can't believe how much just one can of formula and one package of diapers cost! The cashier looked at me like I was insane. When I asked for a pen, her expression changed from obvious disbelief to sheer panic.
Cashier: "Are you going to write a check?"
Me (I hate obvious questions): "That was the plan."
Cashier: "Uhhh"
Me: "You do accept checks, right?"
Cashier: "Uhhh… I'm not sure. They didn't cover this in training"
She had to call a manager over. The manager had to consult with someone over the phone. They consulted other cashiers and eventually I was able to write my check.It made me wonder… am I really the last of a dying breed? I'm not a fan of the paper check… but at the same time, shouldn't stores be equipped to take all forms of payment… or if they aren't, have it posted on the doors and at the registers? The whole ordeal took nearly 10 minutes… but more annoying than that, I left feeling alienated.
Of course, I still haven't gone in to get a debit card to that account because it requires both of us to be there… and when we have time together, we don't want to go to the CU (sorry Verity!). I'm not sure I really have a point… and I know it's taken me a long time to get to the end of a pointless blog. I guess I just felt like sharing my thoughts on the paper check and the experiences I've had surrounding them lately. Perhaps if anyone associated with Walmart is reading this… they could put in a request to include the acceptance of paper checks into their training."
What do you think? Will paper checks go the way of the dodo bird or are they here to stay?
BTW, I emailed my friend and told her she didn't need to go into a branch to get a debit card. She commented back on her blog—thanks for the shout out, K!
"Kayce said… Now that's customer service! Granted, I used to work at the Credit Union… but a staff member there read my blog and took it upon herself to email me and let me know that I can call to have a debit card issued on the joint account… no need to go into the branch!Take note Walmart! She wasn't sure what the proper procedure was… but instead of trying to talk me out of using the debit card, she asked around and got me some answers. I like that about this company. The employees take it upon themselves to take care of their members and make them feel important. If you are in need of a financial institution, I'm still a big fan of Verity Credit Union. "
Shameless plug, I know—but seriously, will paper check usage ultimately be replaced by debit cards and online bill pay?
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From an emblem of patriotism, to the shoulders of Hollywood stars and now the staple piece of your winter wardrobe, we explore how the trench coat became an icon of fashion.
A Symbol of Patriotism
The trench coat coined its name during World War 1. Wanting to establish status and the social hierarchy of the military, the trench coat was originally designed for soldiers. Double breasted and tailored to the waist, the coat had to be able to withstand the wind, rain and harsh war conditions in the trenches.
From the Trenches into the Fashion world
Stepping out of the trenches and into department stores, men's fashion soon enough adapted the soldier's coat into an ageless fashion trend. Men across America were wearing trench coats to show their solidarity with the war effort. However, in a time where women were encouraged to show their patriotism through domestic household roles, strong, female actresses were emerging in Hollywood. These celebrities sought to challenge such roles and show their support through fashion, adopting the trench coat look. This marked the very early stages of female empowerment, as women began to delve into man-tailored, female dressing.
The entertaining and talented Greta Garbo gave the stamp of approval on male influences on female dressing, during her performance in A Woman of Affairs. Her stunning trench coat, lined with tartan wool and cinched at the waist inspired women around the world. Many female celebrities followed suit, expressing their admiration for the military through upright collars and belted waists. Audrey Hepburn's classic trench coat styling in Breakfast at Tiffany's has continued to be interpreted into modern designs to this day.
A Modern Era for the Iconic Trench Coat
Fast forwarding over a century, what was once a sign of national patriotism is now an essential style for any worthy wardrobe. Despite being more than 100 years old, the trench coat has managed to maintain its place at the peak of high fashion. At its core, the trench still upholds its classic tailoring at the waist and double-breasted form. However, you can make it your own with a pop of colour, experimenting with different collars or alternating between buckle and waist ties for a timeless look, no matter the season. Paired with a structured dress or thrown over your favourite denim, the trench is as versatile as it is classic.
Images sourced from pinterest.com.au


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Brothers on song
The Malladi Brothers are indeed a breath of fresh air when they ascend the concert stage. With their foreheads streaked with bright splashes of vibhuti, they look bright and sparkling in their usually pearly white jibbas and veshtis, their eyes taking in the audience in an intelligent sweep that seems at once to gauge its level of appreciation, its stylisitic preferences. They invariably radiate energy and grandeur even as they deliver the opening salvo in a brisk, bright manner that would have done credit to the Alathur Brothers, arguably the most spectacular duo in Carnatic music.
With their impeccable pedigree, Sreeramaprasad and Ravikumar are no doubt destiny's children. With both father and guru devout students of a great musician/ musicologist/ teacher, could they have chosen a career path other than Carnatic music? Eclectic is a much abused cliche, but in their case, that seems the only word to describe the several influences that have shaped the siblings' musical loyalties as much as their readiness to embrace art from different directions, with good aesthetics the only limiting factor… read more


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Using genome-wide microarrays, we recognized 172 genes that are highly expressed at one stage or another during multicellular development of Dictyostelium discoideum. When developed in shaken suspension, 125 of these genes were expressed if the cells were treated with cyclic AMP (cAMP) pulses at 6-min intervals between 2 and 6 h of development followed by high levels of exogenous cAMP. In the absence of cAMP treatment, only three genes, carA, gbaB, and pdsA, were consistently expressed. Surprisingly, 14 other genes were induced by cAMP treatment of mutant cells lacking the activatable adenylyl cyclase, ACA. However, these genes were not cAMP induced if both of the developmental adenylyl cyclases, ACA and ACR, were disrupted, showing that they depend on an internal source of cAMP. Constitutive activity of the cAMP-dependent protein kinase PKA was found to bypass the requirement of these genes for adenylyl cyclase and cAMP pulses, demonstrating the critical role of PKA in transducing the cAMP signal to early gene expression. In the absence of constitutive PKA activity, expression of later genes was strictly dependent on ACA in pulsed cells.
Multicellular organisms develop through a series of stages during which the patterns of gene expression are regulated to alter the properties of the cells and generate distinct cell types. These processes can be clearly recognized during development of the social amoeba Dictyostelium even though multicellularity is achieved by aggregation of vegetative cells to give the multicellular forms (5, 15, 19). During the first 10 h following the initiation of development, previously solitary amoebae acquire the ability to signal each other by secreting cyclic AMP (cAMP) and respond chemotactically to form mounds containing up to 105 cells. Definition of the genetic program that underlies these and subsequent developmental changes can benefit from genome-wide expression analyses with wild-type and mutant strains. Characterization of a large number of developmentally regulated cDNAs by the Japanese EST (expressed sequence tag) Project (28) has made it possible to recognize patterns of temporal and cell-type-specific expression of many developmentally regulated genes by use of microarrays (14, 25, 38). Microarrays not only expand the number of genes analyzed but also allow direct, quantitative comparisons between different genes.
A considerable number of genes are known to be expressed at high rates soon after the initiation of development (17, 20, 23, 26, 27, 29, 30). These include the genes for the cAMP receptor (carA), for the Gα subunit that couples the receptor to adenylyl cyclase (gpaB), for the activatable adenylyl cyclase (acaA), and for the secreted and internal cAMP phosphodiesterases (pdsA and regA) (3, 11, 29, 34). The products of these genes function together with preexisting proteins to generate an oscillatory network that relays extracellular cAMP signals and regulates the levels of cAMP both inside and outside the cells (12, 18, 21). As the cells aggregate in chemotactic fields, they stick to each other in an EDTA-resistant manner as the consequence of expressing the genes, csaA and lagC, that encode the surface proteins gp80 and gp150, respectively (13, 40).
It has been known for some time that cells will express many developmental genes while suspended in buffer if they are given pulses of 30 nM cAMP at 6-min periods for several hours followed by high concentrations of cAMP to mimic conditions encountered on solid supports (17, 23, 26, 27, 30). Under these conditions, the cells are spatially homogeneous and can be expected to express developmental genes more synchronously than when developing on solid supports where there are local differences in cell density. Moreover, the effects of cAMP pulses on gene expression can be directly addressed. We compared the expression profiles in cells developing on solid supports with those in cells incubated in suspension with or without the addition of cAMP pulses. We found that most of the genes that are strongly expressed during the aggregation and mound stages of development are normally expressed in cells incubated in buffer and given cAMP pulses.
Three genes, car1, gbaB, and pdsA, have been shown to be expressed in suspension-developed cells even in the absence of cAMP signaling (10, 17, 23, 26, 27). Their products allow cells to respond to cAMP and regulate its exogenous levels. Surprisingly, these were the only cAMP pulse-independent genes recognized out of 6,345 targets carried on the microarrays.
Growth, development, and preparation of RNA.Strains AX4 (wild type), GP6 (acaA−), TL149 (acaA− acrA−), and TL130 (acaA− acrA− pkaCoe) were grown axenically in HL5 medium and collected while in exponential growth (2, 29, 37). Strain GP6 was generated by homologous recombination in the acaA locus of AX3-derived strain JH10 and was the kind gift of Peter Devreotes. Strain TL149 was the kind gift of Fredrick Soderbom, who used homologous recombination to delete acrA from strain GP6. Strain AK631 (acaA− pkaCoe) was provided by Adam Kuspa.
For development on solid supports, cells were collected, washed, and deposited on nitrocellulose filters on buffer-saturated pads and allowed to develop synchronously at 22°C (37). Cells were scraped off the filters at 2-h intervals into deionized water, pelleted, and dissolved in Trizol reagent (Gibco/BRL), and total RNA was prepared.
For development in suspension, exponentially growing cells were collected, washed, and resuspended in 100 ml of 20 mM Na+ K+ phosphate buffer (pH 6.5) at a concentration of 107 cells/ml and rapidly shaken at 125 rpm on a rotary shaker. To simulate normal signaling, cells were given pulses of 30 nM cAMP at 6-min periods from 2 to 6 h followed by addition of 300 μM cAMP at 2-h intervals (30). At each time point, 5 × 107 cells were collected, pelleted, and dissolved in Trizol reagent (Gibco/BRL) for preparation of RNA.
Preparation of microarrays.Corning slides were microarrayed with 6,345 cDNA and genomic targets by using a Molecular Dynamics GenIII robot in the BioGEM facility as previously described (14). Inserts from 5,655 cDNA were generously provided by the Japanese EST Project (28). A list of the genes is available online (http://www.biology.ucsd.edu/loomis-cgi/microarray/paper2.html ). All genes referred to in this study were sequence verified.
Expression analyses.Probes were prepared from total RNA collected at 2-h intervals as well as from time-averaged reference RNA as previously described (14). Superscript II DNA polymerase (Invitrogen, Carlsbad, Calif.) was used to incorporate either Cy5- or Cy3-conjugated dCTP (Amersham, St. Louis, Mo.) into DNA. Following incubation at 42°C for 3 h, unincorporated dyes were removed using Microcon-30 columns (Millipore, Burlington, Mass.) with three washes with 450 μl of Tris-EDTA buffer before drying and resuspension in 5× SSC (1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate)-0.3% sodium dodecyl sulfate-25% formamide. Labeled probes were mixed and hybridized at 42°C to the microarrays for 6 to 12 h. Dyes for the sample and reference probes were interchanged in different experiments. Protocols are available online (http://www.biology.ucsd.edu/loomis-cgi/microarray/index.html ).
Probed microarrays were analyzed in an Axon Genepix 4000B scanner, and the measurements were processed with the associated software. Total Cy3 signal was normalized to total Cy5 signal after background subtraction to allow independent slides to be compared. The ratios of Cy3 to Cy5 for individual genes were then calculated. Each sample was hybridized to two or more microarrays. Each developmental time course was repeated at least twice. Mean values were used for subsequent analyses and are available online (http://www.biology.ucsd.edu/loomis-cgi/microarray/paper2.html ). Values were normalized to 1 at the start of each experiment.
Statistical analyses.The median and standard deviation of the time course values for each target were analyzed by a curve-fitting algorithm to extract the times of onset and cessation of transcription (31). The goodness of fit was measured by the statistic s = Σ[E(t) − A(t)]2/Σ[E(t) − 1]2, where E is the ratio of the background-adjusted fluorescence of the time sample (Cy3) to that of the reference sample (Cy5) normalized so that the mean expression level of each gene throughout development is unity and A is the abundance of the gene transcript at time t. Since the denominator of the statistic is a measure of temporal variation found experimentally, s is a measure of how much of that variation can be accounted for by the kinetic equation. The statistical significance (P value) of the s statistic for each gene was calculated by permuting the expression pattern 1,000 times and calculating the fraction of permutations whose s statistic is less than that of the experimental pattern. Genes with good statistics that showed an increase of at least fivefold during development were K-means clustered into five groups that were consistently color coded. Averages of the clusters were normalized to the time of initiation of development (t = 0).
Expression profiles during multicellular morphogenesis and in suspension.To characterize a large proportion of the genome, we arrayed 5,655 targets from cDNAs provided by the Japanese EST Project together with 690 targets previously chosen from specific genes (14, 28). Exponentially growing cells of wild-type strain AX4 were developed on filter supports, and samples were collected every 2 h. Fluorescently labeled copies were generated from RNA of each time sample and compared to labeled copies generated from time-averaged reference RNA prepared by pooling samples from different stages in development as previously described (14). Signals at 5,279 of the 6,345 targets either decreased or failed to increase significantly during development. These were mostly vegetative genes that are shut off during development. Values for each individual gene throughout development are available in supplemental materials found online (http://www.biology.ucsd.edu/loomis-cgi/microarray/paper2.html ).
We have focused on genes that give at least a fivefold-higher signal at one stage or another during filter development of wild-type cells. Many of these genes are represented by several targets giving good reproducibility. A nonredundant set of 172 developmental genes clustered into five groups on the basis of their temporal profiles (Fig. 1A.). A cluster of 24 genes started to accumulate immediately after the initiation of development, another cluster of 42 genes started to be expressed at 4 h, another with 23 genes accumulated after 6 h, and two clusters started to accumulate at 8 h. Expression profiles for genes in the first four clusters were similar in cells incubated in buffer to which cAMP pulses were added for 6 h followed by high levels of cAMP (Fig. 1B). Many of the genes in the fifth cluster that were expressed after 8 h of development failed to be expressed in cells treated with cAMP in suspension, suggesting that they require intercellular signals in addition to cAMP.
In all, we found 125 targets that gave robust developmental signals in cells developed in suspension and treated with cAMP. In the absence of cAMP treatment, very few genes were expressed (Fig. 1C).
Expression profiles of early genes.The expression profiles for 18 genes (Table 1) that started to accumulate within the first 2 h of development in suspension are shown in Fig. 2. Except for carA, which encodes the high-affinity cAMP receptor, most of these genes were expressed at reduced or insignificant levels unless the cells were treated with cAMP pulses (Fig. 2A and B). When developed in suspension, the cells were at sufficiently high density that accumulation of cAMP could result in autonomous signaling after a few hours (12). Therefore, we analyzed a mutant strain, GP6, lacking the cAMP activatable adenylyl cyclase, ACA.
In the absence of cAMP treatment, only four genes, carA, gpaB, pdsA, and cbpB, were expressed in cells lacking ACA (Fig. 2C). Three of these genes, carA, gpaB, and pdsA, have been previously shown to be expressed in cells developed in suspension in the absence of cAMP and at higher levels when the cells were treated with cAMP pulses (10, 17, 23, 26, 27). cbpB is unlikely to be a pulse-independent gene since it did not significantly accumulate in unpulsed wild-type cells (Fig. 2A).
Treatment of acaA− null cells with pulses of cAMP resulted in expression of all of the early pulse-dependent genes, except for acaA, which is mutated in these cells (Fig. 2D). cAMP induction of these genes is somewhat surprising since the signal transduction pathway leading from exogenous cAMP to alteration in the transcriptional pattern is thought to be mediated by PKA following stimulation by a rise in internal cAMP (4, 21, 32, 39). Yet these acaA− cells lack the major source of internal cAMP, the adenylyl cyclase ACA that is coupled to the cAMP receptor.
Genes that were expressed later in wild-type cells while suspended in buffer and treated with cAMP pulses failed to be expressed in pulsed acaA− cells (Fig. 3). Expression of these late genes appears to depend on activation of ACA to generate high levels of cAMP within the cells.
Role of the minor adenylyl cyclase ACR.The induction of early genes by cAMP in cells lacking ACA suggests that other signal transduction pathways may be stimulated upon ligand binding to CAR1. One such pathway involves a MAP kinase cascade that leads to the rapid activation of the MAP kinase ERK2 (24, 33). Mutant strains lacking ERK2 fail to accumulate cAMP and so are unable to aggregate. However, this phenotype can be suppressed by mutations in the gene encoding the internal cAMP phosphodiesterase, RegA (22; A. Kuspa, personal communication). These cells accumulate high levels of cAMP and are able to form aggregates by accretion. Thus, it appears that when ERK2 is activated by external cAMP binding to CAR1, RegA is inhibited and cAMP can accumulate to levels that activate PKA. However, there must be a source of cAMP other than ACA since at least 14 genes are activated by exogenous cAMP pulses in acaA− null cells.
There is a minor adenylyl cyclase activity in vegetative cells that could be the source of cAMP (16, 35). To test the hypothesis that this adenylyl cyclase is necessary for pulse induction when ACA is missing, we characterized suspension-developed cells of a strain in which both adenylyl cyclase genes, acaA and acrA, are disrupted. As shown in Fig. 4, the early pulse-independent genes were expressed with or without cAMP pulses but none of the pulse-dependent genes were expressed at significant rates even when the double mutant cells were treated with cAMP. Thus, internal cAMP appears to be required for expression of these genes. Characterization of a strain in which only acrA is missing showed that either ACA or ACR is sufficient since all early genes were expressed in pulsed acrA− null cells (see the supplementary materials available online).
Constitutive PKA activity bypasses the requirement for internal cAMP.If increases in the internal levels of cAMP regulate gene expression by stimulating PKA activity, then constitutive PKA activity should be sufficient for full expression independently of cAMP synthesis. The catalytic subunit of PKA is inhibited when associated with its regulatory subunit but is activated when cAMP binds to the regulatory subunit. However, when the catalytic subunit outnumbers the regulatory subunit, some of the PKA activity would be expected to be cAMP independent and constitutive (1). We disrupted acrA in strain AK631, in which acaA is disrupted and actin 15/pkaC is carried in a multicopy vector. The resulting strain, TL130, was shown to have high levels of PKA activity that was independent of cAMP (2). Early genes, both pulse independent and pulse dependent, were fully expressed in both unpulsed and pulsed cells of this acaA− acrA− pkaCoe strain that is unable to synthesize cAMP in response to exogenous cAMP pulses. As expected, acaA was not expressed since it is mutated in this strain. It appears that constitutive PKA activity is sufficient for expression of these genes.
Previous Northern analyses of carA and gbaB have indicated that these genes are expressed at basal levels in the absence of cAMP pulsing and at a higher rate when exposed to cAMP pulses (17, 23, 26, 27). Results from the microarray analyses confirmed these observations but showed that pulsing resulted only in a modest two- or threefold increase in accumulation of these mRNAs (Fig. 2 and 4). Even when one or both of the developmental adenylyl cyclases were disrupted, the pulse-independent genes were expressed within the first few hours of development.
When development is induced by shifting the cells to a nonnutrient environment, carA and gbaB mRNAs start to accumulate immediately such that their products, the cAMP receptor CAR1 and the trimeric G protein subunit Gα2 that couples it to activation of ACA, are rapidly synthesized. The cells then become responsive to cAMP and express these genes as well as pulse-dependent genes at high levels. The only other pulse-independent gene that was recognized on the microarrays, pdsA, encodes the extracellular cAMP phosphodiesterase. Previous studies have shown that transcription of pdsA is regulated by three independent cis-acting regions. The distal promoter is activated in the first few hours of development in a pulse-independent manner (10). Rapid synthesis of the phosphodiesterase allows the cells to keep the extracellular levels of cAMP within bounds as they start to produce cAMP pulses. Strains carrying null mutations in either carA or gpaB fail to generate cAMP pulses or show any signs of aggregation when developed on solid supports (29). Likewise, strains carrying null mutations in pdsA fail to show chemotactic movement (36).
A set of pulse-induced genes was coordinately expressed starting at 2 h of development such that their mRNAs reached high levels by 6 h of development. Most of them declined thereafter when the levels of exogenous cAMP were raised. These genes were induced by cAMP pulses even in cells lacking the cAMP-stimulated adenylyl cyclase, ACA (Fig. 2D). Later genes were not expressed in acaA− null cells (Fig. 3), suggesting that they depend on the high internal levels that ACA can generate.
acaA is expressed rapidly in pulsed cells and leads to a high rate of cAMP synthesis during the aggregation stage. Another pulse-induced gene, csaA, has previously been shown to be cAMP regulated (9). It encodes the cell adhesion glycoprotein gp80. There are five pulse-induced genes that encode calcium binding proteins, cbpA, cbpB, cbpD, cbpF, and cbpG; cbpB becomes enriched in prespore cells while cbpD becomes enriched in prestalk cells (14). Although cbpB was expressed in unpulsed acaA− cells, it was not expressed in unpulsed wild-type AX4 or acaA− acrA− double mutants and so is unlikely to be a pulse-independent gene. Loss of cbpA results in aberrant aggregation, further indicating a role for calcium regulation in early development (7). SSL579 encodes a protein related to CbpB and other calcium binding proteins and has four EF-hand domains.
dia-2 encodes a protein of unknown function which is essential for normal aggregation (6). pgmA encodes phosphoglucomutase, which plays a role in rapid breakdown of glycogen during early development (19). Three members of the lagC family are pulse-induced genes. The product of lagC, gp150, is a surface glycoprotein which makes cells mutually adhesive at the mound stage and is necessary for later gene expression (8, 40). lagC2 encodes a protein which is 55% identical to LagC and may play a similar role. Two novel genes from the EST collection, SLD188 and SSG481, were found to be expressed in pulsed wild-type and acaA− null cells (Table 1).
The signal transduction pathway leading from CAR1 ligand binding to activation of these genes appears to require a source of internal cAMP to activate PKA. Only when both acaA and acrA were disrupted did cAMP pulses fail to induce these genes (Fig. 4). While the stimulation of ACA upon ligand binding to CAR1 is well defined, it has not previously been realized that ACR is able to generate sufficient cAMP for PKA activation in pulsed cells. A model accounting for the kinetic interactions of these components following a pulse of exogenous cAMP is given in Fig. 5. Ligand binding to CAR1 leads to GTP replacement of Gα2-bound GDP and dissociation of the trimeric G protein. In wild-type cells, the βγ subunits lead to activation of ACA while the Gα2 subunit leads to activation of the MAP kinase ERK2 (4). If ERK2 results in the inhibition of the internal phosphodiesterase, cAMP can build up and activate PKA. It has been proposed that PKA activity indirectly leads to inhibition of ERK2 and the reactivation of RegA (4, 18, 21). Even in the absence of ACA, this model can account for the activation of PKA in cells treated with exogenous pulses of cAMP.
On a longer time scale, expression of pulse-independent genes within the first few hours following initiation of development can be seen to prepare the cells for expression of pulse-dependent genes. Entrainment of pulse production among cells ensures that they will be coordinated for subsequent differentiation (12, 18). At least one pulse-dependent gene, lagC, is necessary for progression through subsequent developmental stages (8; our unpublished data). Strains carrying lagC null mutations form only loose aggregates and fail to express postaggregation marker genes even when treated with cAMP. Other pulse-dependent genes may also play critical roles in the temporal sequence of gene expression. Genome-wide expression analyses can uncover previously unsuspected genes within coordinately controlled groups and focus attention on their physiological roles. The newly recognized developmentally regulated genes behaved in a consistent manner in the different strains under various experimental conditions and provide robust markers for the early stages of development. It will be interesting to define later groups on the basis of genome-wide expression profiles in strains lacking specific gene products and determine the networks that interconnect them.
We are indebted to the Japanese EST Project for supplying inserts of cDNA clones and the BioGEM facility of the University of California, San Diego for arraying them. We thank Nicolas Buchler for statistical analyses.
This work was supported by grants from the National Institutes of Health (GM60447 and GM62350) and the National Science Foundation Biocomplexity Program.
- Received 28 March 2003.
- Accepted 5 June 2003.
- Copyright © 2003 American Society for Microbiology


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Rex Pemberton - Youngest Australian to climb Mt Everest & the Seven Summits
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Rex? Simply Exceptional. The best keynote I have ever attended.
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Thank you, Tim, that was a truly funny, motivation- al experience... a presentation built for the moment. Looking forward to working with you ...
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Rowan Gibson's motivation and leadership have changed the dynamics of our organization.
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artist's notes
January 6, 2019
I'm staying warm today, and working inside the studio. I still manage to share my day with the goats, one way or another. Maggie may be a momma this coming May. Time will tell.
September 30, 2018
Every Summer Solstice a group of musicians from all over the Rocky Mountains converge on a special place for a weekend of playing music, great conversation, reconnecting and starlit nights around the campfire. This weekend leads to new musical compositions, new friend...
November 15, 2017
An apple a day and a drawing a day is where I am these days. Apples are in abundance these days as I'm surrounded by beautiful organic orchards.
A drawing a day seems abundant as well. After months of stepping back from my work, pausing and reflecting on the "whats" an...
November 11, 2017
She's my studio mate, my lap warmer, and my free entertainment. But when the light catches her whiskers, and the silly fluff of her hair...she's my model and muse. It's the simple things like this that make me question why joy can sometimes elude us.
November 9, 2017
It really depends on how fast you are flying and at what angle you hit the window. That split second decides your fate. I hear the hit. It's unmistakable. I check to see if there's any hope. Sometimes you revive, I'm relieved and I sing a little joyful song. And somet...
August 18, 2017
I'm working on a small commission of a Western Meadowlark today. There is a pair who nest in my goat pasture every year. Their nest is usually under a tuft of loose grass, barely hidden. In the past, if I can find it, I put a
wire barrier around it so I don't step on...
May 7, 2017
My most recent painting is titled "Home". It originated from a vision I kept having of my husband's beard, and the many forest creatures that live around us. But it then became more personal and as I was painting his image, the birds kept flying around it (in my head...
April 28, 2017
The studio space that I share with two other artists celebrated it's open house last night. What a great crowd, great support, and great food...oh yeah, and the art was pretty great too. It's an old turn of the century apartment above the Blue Sage Center for the Art...
February 13, 2017
Happy Valentine's Day!
In honor of love, "Sweetpea's Day" will be hanging in the beautiful Blue Sage gallery in downtown Paonia, Colorado. I have two of my large goat paintings up for another couple of weeks. I love walking by the windows and seeing the girls looking great on the walls of...
January 21, 2017
My large canvases were photographed and archived by Brian Maul of West Slope Graphics. It's great to see Brian amongst the goats. There will be some Fine Art Giclees of these paintings coming soon!
"Maggie in the Snow"
The Source
An apple a day
Me-You kitty.
You brought beauty...
Meadowlark, Meadowlark, what do you see?
Home is a funny thing...
Second Story Studios in open!
Goats Love Love
Got your goats!
January 2019 (1)
September 2018 (1)
November 2017 (3)
August 2017 (1)
May 2017 (1)
April 2017 (1)
February 2017 (1)
January 2017 (2)
July 2016 (1)


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Tag Archive
another dead comedian biff juggernaut BLC Productions blight the clown blood in vain bret hart chvad chvad sb clowns compilation devo elias facility records god in a box Go Kustom Records horror culture Industrial Nation interview invisible records liar live lyrics mettle mettle III new york nyc raphael hickman recherche remix review sealed in silence show show dates site updates so emotional tao spudsuckers t.o.t.s. Teen Feeding Frenzy: A Tribute to the Music Teens Love the most appealing thing the qualia things outside the skin tots various artists video you knew it all along
Ectomag, interview (2005)
Publication: Ectomag t.o.t.s. Interview w/Ectomag (Oct. 2005) by; Sameerah Ecto/Sameerah: Fucks sake, It's been another shit day at my day job and once again I am running late. These days it seems like the story of my life. Tonight it isn't a rush to get home and feed the cat or a potty emergency that […]


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New issues show Facebook's loss of control
Facebook has exploded into the news this week with a number of issues that show how problematic the popular platform has become. The first issue for Facebook was when they announced the deletion of a number of profiles and pages that were from Russia and Iran designed to spread false and inflammatory information into the US and other Western countries. Facebook says they deleted 652 pages, groups, and accounts. While it's commendable that Facebook removed these accounts, this is only a symptom in a larger problem of continuous foreign influence in Western Democracy.
Secondly, Facebook announced that, in a move similar to the Cambridge Analytica scandal, four million users may have had their personal data compromised. Facebook has banned an app called MyPersonality, a personality quiz as you can probably surmise. Even though four million users is a drop in the bucket of Facebook's billions of users worldwide, that's still a significant number of users whose personal information may have been exposed. While it's an improvement over the 87 million accounts exposed in the Cambridge Analytica kerfuffle, Facebook seems like it's still leaking like a sieve with our information.
Lastly, and probably the most damning, the New York Times published an expose on a study that has linked Facebook use to anti-refugee violence in Germany. While the study doesn't blame Facebook per se, it does allege that Facebook use paired with engagement into hate-filled rhetoric has resulted in a rise in hate-related violence. What concerns me most about this study is how much Facebook hate-crimes based on ethnicity or religion are coming closer and closer to the US. In today's charged political climate, how long will it be before Facebook lynch mobs finally leave their keyboards and start taking to the streets?
Facebook has become a virus that has escaped the lab and is creeping through the world's population and by continuing to rely so heavily on it we're willingly ignoring the cure.


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Joint Scholars' Convocation
18 March 2016
Institute of Biology, National Science Complex
University of the Philippines, Diliman
Quezon City 1101
Valedictory address of Mr. Miguel Ricardo Leung
Mr. Leung is the graduating valedictorian, summa cum laude, for this year's batch in the UP System
Good afternoon, everyone.
When I was asked to give this speech, I was given the topic "academic excellence in future service". As a science major, I have not been asked to write or speak about anything remotely related to this in recent memory. I proceeded to do what any scientist (and any UP student for that matter) would do when faced with something unfamiliar – I googled it. To my dismay, many of the search results instead talked about how academic excellence does not determine one's future.
This idea – that academic excellence does not in any way predict one's future success – is one I have heard echoed by students and faculty throughout the University, as well as by professionals outside its confines. They say that grades don't matter. They say that the students who will really succeed in the real world are not those who spent their free Friday nights poring over their favorite textbook.
Such ideas may come about from falsely associating academic excellence with something it is not. Academic excellence is not about getting high grades. To me, academic excellence describes the pursuit of knowledge – the desire to understand. It is the unrelenting drive to discover and to learn, to devote oneself to unraveling the intricately woven threads of existing human knowledge. What makes such scholarly pursuits worthwhile is that by taking stock of what we know, we one day arrive at the realization that there is much more that we do not know. Academic excellence, therefore, is the process, not the product – it is the race, not the medal.
To me, academic excellence means being an active learner. It is not enough to accept the information that is provided to us by our mentors, our peers, or the media. This, to me, is where academic excellence and simple grade consciousness diverge. It is highly possible to get excellent grades by simply accepting information, determining what is important, regurgitating it on an exam, and then forgetting about it afterwards to make room for the next subject.
But this is not how academic excellence works. It is those who look at the information, take a step back, and dissect it – pull it apart, turn it on its head, beat it, batter it, torture it – who will truly gain an understanding of the material. It is in the process of doubting, questioning, and rebutting ideas that we begin to understand where they fit in the context of other ideas. Academic excellence is not the mere acceptance of information, but the screening and distilling of ideas to understand what is truly fundamental. Academic excellence is not being afraid to stop and think.
These are the attitudes developed in students who are academically excellent. But how do these scholarly attributes help individuals contribute not just as academics, but as citizens? I would like to illustrate this with an example that hits close to home.
In December of last year, the Supreme Court decided to permanently stop government field tests of genetically modified Bttalong. In the said ruling, the SC also temporarily stopped the government from accepting new applications for field testing or propagating GMOs. The SC cited evidence from Greenpeace that there is no scientific consensus on the safety of GMOs, and one of the major studies cited was from a Greenpeace-funded study that was retracted from the journal that initially published it because of questionable and unsound methods and analyses.
A thorough evaluation of the prevailing scientific literature on GMOs shows that there is a clear scientific consensus that they are safe, and that they do provide benefits of increased yield and decreased dependence on chemical pesticides. If the attitudes of academic excellence and scholarly passion were applied to this case, then perhaps we would have had a different outcome. Agriculture is one field where the Philippines had a real, fighting chance of becoming regionally if not globally competitive. Instead, agricultural biotechnology has been set back – we may ultimately have to depend on other countries to feed our population.
I believe this situation exemplifies the role of academic excellence in service to the country. As soon-to-be UP graduates, we will become professionals, and many of us will go on to become mid- or even high-level decision-makers. It is my sincerest hope that we will carry with us the attitudes of academic excellence when we get there – the attitudes of critically evaluating evidence, thinking logically and rationally, and synthesizing information to understand the driving forces behind events.
We must remember, however, that our academic pursuits – whether in the natural sciences, the social sciences, or the humanities – can never be separated from the societal contexts in which they operate. Remember that what sets a UP student apart is not academic excellence, but the desire to use this excellence in the service of the country and of the people. Kaya nga UP – utak at puso.


text: Boo Radley by KYOSI We are doing well for literary references this week and KYOSI does her bit to make words mean more with her song "Boo Radley". She is right on the money musically with her insistent electronica neatly counterpointing her words. www.facebook.com/kyosimusic Review date: May 24 2019 Reviewer: Bluesbunny


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All members of Serampore Rifle Club are hereby notified that a club picnic has been scheduled on 13th January, 2019, Sunday. Here are the following details below:-
- Date: 13 January, 2019
- Venue: Serampore Rifle Club
- Time: 9:00 AM
- Menu (Breakfast): Koraisutir Kochuri, Aloor Dom, Dorbesh
- Menu (Lunch): Veg. Fried Rice, Chili Chicken, Chili Paneer (for vegetarians only), Salad, Chatni, Papad
- Snacks (Evening): Veg Pakoda, Coffee.
Subscription fee: 250/- per head.
Last date of subscription: 06/01/2019 (Sunday).
Interested persons are requested to collect the coupon from SRC office.
N.B.: Any member without a picnic coupon will not be catered in the picnic.
Cub picnic on 13th January, 2019


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'Masterpieces of Chinese Painting' opens in LondonComments
Rare Chinese artefacts, dating as far back as 1,200 years, are going on display at the Victoria and Albert Museum in London.
The exhibition presents over 70 of the finest examples of Chinese painting, some of which have never been exhibited in the UK before.
It begins with paintings from the Tang Dynasty and includes 'The Nine Dragons' hand scroll that captures the world of the mythical fire-breathing monster.
Outlining the developing styles of painting over a 1,200-year period, the exhibition also includes figure paintings on silk for religious sites and landscape painting.
The exhibition is divided into three parts: the very early period, when most of the art was concerned with deities and the symbolism of costume; the middle period, which focussed on nature; and the 19th century, when people's faces became important in portraits.
Senior curator at the Victoria and Albert, Hongxing Zhang gave an insight into the development of Chinese art:
"In Chinese painting there is a genre we could describe as similar to genre paintings in European history. (It) really started in the 12th century, but became very popular and one of the dominant, major genres of painting in the 15th and 16th centuries. But, the highest achievement of the genre painting was the court art in the 18th century."
According to the Chinese, painting is a supreme art – something that is in clear evidence throughout the exhibition.
This is the biggest exhibition of Chinese art in the UK since a Royal Academy exhibition in the 1930s. These masterpieces shed some light on China's past and help understand Chinese society today.
Zhang also spoke of the importance of the exhibition in understanding China and he Chinese:
"China is increasingly exposed in the west for many, many reasons: the economy is a very major one. And so, in order to do business and to have a dialogue with Chinese people we need to know about China and know about really, the stories behind a contemporary China."
Chinese life is represented in miniature throughout the exhibition; from streets teaming with merchants, to a fishing village and boats on the shore at the edge of the town.
It took years to complete and contains over 4,600 figures and 400 boats.
The exhibition will continue until January 19, 2014.


text: > > At least 3 South African species have brightly orange to >yellow flowers ( O dubium, O maculatum and O. multifolium). > > The wiki page >(http://pacificbulbsociety.org/pbswiki/index.php/…) >shows some hybrids . but they seem to be all South African too. > > Are there any hardy hybrid Ornithogalum ythat are any color >other than same-old-white? Jim, I realise this is not what you'd call hardy, but the Ornithogalum dubium (yellow form) I have sail through -8 or -9'C without a problem. I think that is hardier than people seem to think of them. I've never lost one of frost so I would hazard that they'll take quite a bit colder than that as well? Mine are out in the open in small pots, and unbothered by the cold at all. I realise that isn't your type of hardy, but it is hardier than I have heard others talk about them at times. I'm so so on the other Orniths, but wouldn't be without the yellow dubium. The orange dubium on the other hand goes dormant and never reshoots again. I've never had one of those come back for a second year (although the bulbs lasted for more than 6 years, still dormant). The yellow form never skips, flowers for months, multiplies, sets seed happily etc. If only I could find one of the orange ones that would do the same. LOL Probably not much help to you given your extreme cold, but might be useful feedback for some other people who are thinking of trying them. Cheers. Paul Tyerman Canberra, Australia - USDA Zone Equivalent approx. 8/9 Growing an eclectic collection of plants from all over the world including Aroids, Crocus, Cyclamen, Erythroniums, Fritillarias, Galanthus, Irises, Trilliums (to name but a few) and just about anything else that doesn't move!!


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They say time heals all wounds, but for Chinese telecom giant Huawei, the hope is that time also leads to significant amounts of forgetfulness as well. You see, Huawei, the third largest smartphone company in the world, has dominated everywhere in the world, except for the in the United States, where over the last several years or so it has been vilified as a communist spy, targeted as a front for foreign espionage, and shunned because of perceived security concerns.
But now, several years later, Huawei is trying once again to enter the saturated American smartphone market, announcing the impending release of its flagship Mate 8 smartphone in 2016.
The hope is, of course, that most people have forgotten the bad press, the witch-hunts, and the veritable tar and feathering that went on through 2012 and 2013, and that consumers will be able to see the Mate 8 (and the cheaper Honor 5X) as worthy competitors to Apple and Samsung's respective flagship devices, comparing the phones on merit, and not what country the company behind the phones hails from.
As mentioned, Huawei stands as the world's third-largest smartphone maker based on shipments, behind only Samsung and Apple in volume. But Huawei has managed to accomplish that with almost no presence in the U.S. mobile market, again due to the vilification of Chinese telecom firms that happened several years ago. The Chinese firm announced this week that it plans to showcase its flagship device at the annual Consumer Electronics Show in Las Vegas early in the New Year, in hopes of establishing itself as a competitor in the high-end smartphone market.
"We have already seized a leading position in the Chinese [smartphone] market. We hope that in the U.S. we can achieve the same success," Huawei Deputy Chairman Ken Hu said in a recent interview.
The vilification of Huawei began in 2012 with a damning Congressional report that recommended avoiding Huawei's telecom hardware because of fears that the company was operating as a front for the Chinese government, and had built in back-doors the communist regime could use to spy on America's industrial complex. While the accusations were largely unfounded (at least to the public) and vehemently denied by Huawei, the company found itself all but shut out from the U.S. market, one of the few global telecom leaders that American consumers had no idea about
But that didn't stop Huawei from attempting the impossible, as the company invested heavily in improving its image and dedicated a research and development team to identifying U.S. consumer preferences and requirements, and working to meet those specifically.
While Huawei has been able to have a small footprint in the budget smartphone market in the U.S., the introducing of the Mate 8 is a sign the company believes the American market is ready to love again, having forgotten all that nasty business about espionage, spies, and whatnot.
Although analysts firmly believe that for Huawei to firmly establish itself as a global smartphone brand that entry into the U.S. market is critical, the fact of the matter is that it won't be easy, as Apple and Samsung clearly dominate, with the remainder of the pie spread across a multitude of smaller brands, meaning that unless Huawei can generate significant market hype, even if customers forget how Huawei has been characterized as in the past, it won't be easy to gain any traction.
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text:
November 27, 2019
Search Committee for System Administrator position in UITS
NWQB 7521, 01:00 pm
Purpose of Meeting: Phone interview.
Contact: Chris Cigale, email@example.com, (414) 229-3410.
This meeting may go into closed session, per state statute:
Wis. Stat. sec. 19.85(1)(c)
Considering employment, promotion, compensation or performance evaluation data of any public employee over which the governmental body has jurisdiction or exercises responsibility., Wis. Stat. sec. 19.85(1)(f)
Considering financial, medical, social or personal histories or disciplinary data of specific persons, preliminary consideration of specific personnel problems or the investigation of charges against specific persons.


text:
Dinner = $12 Includes choice of entree, dessert, and beverage (coffee, tea or soda). Full bar available for additional cost.
Neil Langlois is a CPA and advisor at Jones & Roth. His tax practice focuses on three main areas: the construction Industry, Real Estate Investing, and Estate and Trusts. He is a veteran of The United States Coast Guard.
He enjoys serving the community and currently sits on the board of the Eugene Estate Planning Council and is the President and Treasurer of the Walama Restoration Project.
In addition to his work as a CPA, he owns and operates several rental properties throughout Oregon, which he has renovated and maintained over the years.
Unless you get the free guest pass for first-time guests
Never been to a meeting before? First Time Guests can attend their first meeting for Free with a complimentary Guest Pass.
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text:
Ballinkillen manager Tommy Comerford has stressed that all their focus is now on Saturday's county SHC semi-final.
Following a much publicised fall out with the county board that saw the club refuse to play their county SHC semi-final against St Mullins on the same weekend that they had a number of players lining out with Kildavin-Clonegal, they were subsequently threw out of the championship and their opponents were handed a place in the final.
An appeal by the club was thrown out, but the St Mullins club has offered to play the semi-final and Ballinkillen are now back in the fray.
Comerford told the Carlow Nationalist that all their focus was now on the field and preparing for Saturday's clash at Netwatch Cullen Park.
"That was the goal at the start of the year. To play a semi-final and hopefully a final as well. Thankfully it is all resolved. It will make a good game," said Comerford.
"St Mullins didn't want to go through without playing a semi-final as well. They don't want to be given games. The lads are in good spirits. We will give it a rattle and hopefully it will be a good game."Tweet


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Vail Valley books: Insight into immigration
VAIL VALLEY, Colorado – As an immigrant herself, Helen Thorpe, who will visit the Vail Valley Tuesday, thought she knew plenty about what is undeniably a very complex issue. Then she wrote a book about it and realized just how different her experience was. Thorpe was born in England, moved to the U.S. as a child and became a naturalized citizen when she was 21. The Denver-based journalist (she's written for The New York Times Magazine, New York magazine, Westword, 5280 and more) recently published her first book, called "Just Like Us: The True Story of Four Mexican Girls Coming of Age in America." The book focuses on four Denver girls – two with legal status, two without – trying to live the elusive American dream. Thorpe spent four years shadowing the girls as they move from high school to college. "I thought I'd had a personal experience of immigration. I watched my family adjust to living in a new country," Thorpe said. "As I got to know the students, I realized their experiences were so much more challenging than mine ever were."Not only was Thorpes family's socioeconomic status significantly different than the four girls, her family also spoke fluent English, her father had a job as an engineer lined up before moving to the states and her mother quickly found work as a nurse."We also had white skin and arrived in a country where the majority of people had white skin," Thorpe said. "I didn't experience what it was like to have black or brown skin."She will talk about that and her book, at The Bookworm of Edwards Tuesday. The talk is set for 6 p.m. and at press time, a handful of tickets were available for the event. "No matter what side of the immigration debate you might be on, 'Just Like Us' addresses issues that we deal with every day here in the Vail Valley and in Colorado," aid Besse Lynch, the Bookworm's marketing coordinator. "Helen Thorpe took on a complex topic when she set out to write this book. The issues that she addresses – identity, immigration, education – are gray areas, to say the least."
Thorpe is married to Denver mayor John Hickenlooper, who just last week announced his candidacy for Colorado governor. She admits she wrestled with the question of how to handle that relationship in the context of this project. "At the beginning, it didn't seem like it would be such a big issue – when I first started reporting on this subject, immigration had not yet become the intensely polarized argument that it is today," Thorpe writes in a Q&A on her Web site – helenthorpe.wordpress.com. "It wasn't at the top of the list of things that voters cared passionately about. So initially I imagined that I could write about the girls without ever mentioning my husband. But everything changed during the years that I was reporting on the girls' lives. The subject of immigration became increasingly politicized, and my husband was drawn into the debate. "For a while, I thought I had to abandon this project, but I couldn't forget about the girls."In the end, Thorpe decided that politics had to be part of the story, too, she said.So far the feedback on her book has been positive, and garnered good reviews from The Atlantic Monthly, The New Yorker, The Washington Post and more."I haven't had anyone say I was a raving liberal or way off base after they read the book," she said. "I didn't sugarcoat anything. I think I described some choices these families made, and the average reader might think they made some bad choices along the way," Thorpe said, pointing out a part in the book that describes how one of the girls' father decided to purchase a stolen identity. "I don't think anyone could read that part of the book and come away thinking I thought that was a good thing to do," Thorpe said.High Life Editor Caramie Schnell can be reached at 970-748-2984 or firstname.lastname@example.com.
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Steinar Engen
Bakgrunn og aktiviteter
Steinar Engen (1946) er professor ved Institutt for matematiske fag, nestleder ved Senter for Biodiversitetsdynamikk (CBD), og tilhører Gruppe for statistikk. Professor Engen er utdannet sivilingeniør ved NTH, linje for teknisk fysikk.
Han innehar også D. Phil. grad fra Department of Biomathematics, University of Oxford.
Vitenskapelig, faglig og kunstnerisk arbeid
Et utvalg av nyere tidsskriftspublikasjoner, kunstneriske produksjoner, bok, inklusiv bokdeler og rapport-del. Se alle publikasjoner i databasen
- (2020) Phenotypic evolution in stochastic environments: The contribution of frequency- and density-dependent selection. Evolution.
- (2020) Spatial scales of population synchrony in predator-prey systems. American Naturalist. vol. 195 (2).
- (2020) Decomposing demographic contributions to the effective population size with moose as a case study. Molecular Ecology. vol. 29 (1).
- (2020) Decomposing demographic contributions to the effective population size with moose as a case study. Molecular Ecology. vol. 29 (1).
- (2020) Spatial covariation of competing species in a fluctuating environment. Ecology. vol. 101 (1).
- (2020) Consequences of barriers and changing seasonality on population dynamics and harvest of migratory ungulates. Theoretical Ecology.
- (2020) Contrasting patterns of density-dependent selection at different life stages can create more than one fast–slow axis of life-history variation. Ecology and Evolution. vol. 10 (6).
- (2019) Ecological dynamics and large scale phenotypic differentiation in density-dependent populations. Theoretical Population Biology. vol. 127.
- (2019) Spatial scaling of population synchrony in marine fish depends on their life history. Ecology Letters. vol. 22 (11).
- (2019) Accounting for interspecific competition and age structure in demographic analyses of density dependence improves predictions of fluctuations in population size. Ecology Letters. vol. 22.
- (2019) Towards a predictive conservation biology: the devil is in the behaviour. Philosophical Transactions of the Royal Society of London. Biological Sciences. vol. 374 (1781).
- (2018) The effect of harvesting on the spatial synchrony of population fluctuations. Theoretical Population Biology. vol. 123.
- (2018) Spatial distribution and optimal harvesting of an age-structured population in a fluctuating environment. Mathematical Biosciences. vol. 296.
- (2018) Spatial scales of population synchrony of two competing species: effects of harvesting and strength of competition. Oikos. vol. 127 (10).
- (2018) Heterogeneity among species and community dynamics – Norwegian bird communities as a case study. Ecological Modelling. vol. 388.
- (2017) Spatial synchrony and harvesting in fluctuating populations:Relaxing the small noise assumption. Theoretical Population Biology. vol. 116.
- (2017) Extinction risk and lack of evolutionary rescue under resource depletion or area reduction. American Naturalist. vol. 190 (1).
- (2017) Interactions between demography and environmental effects are important determinants of population dynamics. Science Advances. vol. 3 (2).
- (2017) Reversal of response to artificial selection on body size in a wild passerine bird. Evolution. vol. 71 (8).
- (2017) Evolution of stochastic demography with life history tradeoffs in density-dependent age-structured populations. Proceedings of the National Academy of Sciences of the United States of America. vol. 114 (44).


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These resources examine approaches other institutions have taken to modifying or expanding the curriculum to enhance the capacities of graduate students for success in the twentieth century job market.
Teaching Career Diversity Through Informational InterviewsThe content and format of career diversity within humanities departments follows no single model. As curriculum committees and graduate …Read More »
Humanities for AllA project of the National Humanities Alliance, the Humanities for All database showcases "higher ed-based publicly engaged humanities initiatives, presenting a …Read More »
Mobilizing the Humanities for Diverse CareersThis piece from Anne Krook encourages humanities graduate departments to focus "on two problems whose fixes are within our …Read More »
Recap: A Tool Kit for Doctoral Student Career PlanningIn May 2017, the MLA's Connected Academics Initiative published a guide for PhD programs and faculty about doctoral student career …Read More »
Preparing for Humanities Careers: Suggestions for Doctoral Students and DepartmentsBy Matthew Woodbury, Doctoral Candidate, Department of History As a historian, when asked to explain what I do, one reply …Read More »
American Historical Association's Career Diversity Faculty ResourcesThe American Historical Association has identified five core skills of historical training – communication, collaboration, quantitative literacy, intellectual self-confidence, and …Read More »
Resources for PhilosophersPart of the mission statement of the American Philosophical Association is the "professional development of philosophers at all levels." Beyond …Read More »
What role should departments play?In this post, Dr. Kristina Markman and Dr. Michael Ryan discuss what role departments have in preparing students for humanities …Read More »
Promising Practices in Humanities PhD Professional DevelopmentThis report presents findings from the 2016-2017 Next Generation Humanities PhD Consortium that is useful for faculty and administrators interested …Read More »
Next Steps for Career Diversity: Diving into Graduate EducationIn the December 2017 issue of Perspectives, the American Historical Association's news magazine, Executive Director James Grossman discussed next steps …Read More »
Fixing Our Job-Market ProblemA new article from Chronicle Vitae discusses important considerations for doctoral programs going forward: "There is no reason that strategic …Read More »
PhD Innovation Grants at Duke UniversityDuke University's Versatile Humanists at Duke program, funded by a major grant from the National Endowment for the Humanities, offers …Read More »
Reimagining the Humanities PhD and Reaching New Publics – Simpson Center for the Humanities, University of WashingtonReimagining the Humanities PhD and Reaching New Publics—launched in July 2015 with the support of the Andrew W. Mellon Foundation—has …Read More »
The Future of the Humanities PhDThis article discusses several well-known problems facing PhD programs in the humanities, including high attrition rates, long time to degree, …Read More »
On the Usefulness of Graduate Student InternshipsA history PhD student at the University of Mexico writes about her skepticism when she first learned about internships for …Read More »
Challenges to Graduate Program InnovationTen years after the MLA called for a transformation in the way that foreign language departments structure graduate curricula, very …Read More »
Professional Development at Northwestern UniversityThe Graduate School at Northwestern University offers small grants for departments, faculty, and graduate students for a range of professional …Read More »
Interview With Leonard CassutoLeonard Cassuto is the author of The Graduate School Mess: What Caused It and How We Can Fix It, a thorough …Read More »
Reforming Doctoral Education, 1990 to 2015: Recent Initiatives and Future ProspectsThis report was commissioned by the Andrew W. Mellon Foundation to provide an historical overview of initiatives to reform graduate …Read More »
Bass ConnectionsDuke University has launched a university-wide initiative to develop connections across disciplines and create a new model for education by …Read More »
UGrow: Graduate Internship Program at University of MiamiThe UGrow (Graduate Opportunities at Work) Program at the University of Miami was established in 2015 to provide professional training and …Read More »
From Being to Doing: Mobilizing the HumanitiesThis piece by Anne Krook, a former academic and U of M professor who transitioned to corporate and nonprofit work, …Read More »
Public Humanities Initiatives at University of WisconsinThe Center for the Humanities at University of Wisconsin supports a number of initiatives designed to connect scholars and their …Read More »
Professionalization Initiatives at VanderbiltThe graduate program in English at Vanderbilt University "recognizes the challenges facing doctoral candidates today and devotes considerable attention to …Read More »
Future Humanities: Transforming Graduate Studies for the Future of CanadaFuture Humanities is an SSRC-funded initiative to transform graduate study in Canada and engage in global discussions about humanities training …Read More »
Grants Seek to Foster a Culture Change in Humanities Graduate EducationA recent Chronicle of Higher Education article describes the growing movement towards better preparation for diverse careers. Large organizations such as the …Read More »
National Endowment for the Humanities Announces Next Generation PhD Grant Recipients for 2016-17On August 9th, 2016, the National Endowment for the Humanities announced nearly $1.7 million in grants to 28 universities as …Read More »
Georgetown University, Reinvent the PhD Course DevelopmentReinvent the PhD at Georgetown University is a program supported by the MLA's Connected Academics. The Course Development Group is …Read More »
Re-envisioning the PhD – Recommendations from National Studies on Doctoral EducationOne of the products of a conference held at the University of Washington in 2000, funded by the Pew Charitable …Read More »
Re-envisioning the PhD – Some Common ConcernsThis 26-page report, based on research that preceded the Pew Charitable Trusts-funded University of Washington conference, includes reflections and concerns …Read More »
Humanists at WorkHumanists at Work is a University of California-wide initiative geared toward humanities doctoral students who are interested in pursuing careers …Read More »
Modern Language Association— Task Force ReportThe body of this MLA report contains a number of recommendations for rethinking the humanities doctorate including rationales for each …Read More »
Penn State Graduate Internship ProgramPenn State's Liberal Arts Graduate Internship Program (GRIP) places humanities and social science graduate students with internship positions in university …Read More »
Reimagining Graduate Scholarship: The Emergence of Paid InternshipsA graduate student at the Graduate Center of the City University of New York discusses the growing variety of paid …Read More »
The Praxis NetworkThe Praxis Network features information about programs at colleges and universities in the US and UK that address the future …Read More »
White Paper on the Future of the PhD in the HumanitiesThis paper, published in 2013 by the Social Sciences and Humanities Research Council of Canada & McGill University, argues for a …Read More »


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hi guys Aashi here again hope you like this chapter and an important thing please read the author's note below . Now enjoy the chapter :
Annika's pov
Happy would be an understatement for what I was feeling right now to find him in front of my eyes, shivaay looking handsome as usual in his blue jacket with jeans complementing his magical orbs…with whom I fell so deep. Who would've thought that me coming to the cruise with my family to face my parents would find him standing with his friends in the hallway while I was with bhai to find Mallika .
A nudge on my shoulder broke my trance I looked up to find bhai giving me a concerned look.
"What happened doll? Why did you stop?"
"Nothing bhai just got reminded something sorry.." I said smiling as I can't hold it back, bhai looked happy to find me smiling which was real and hugged me smiling and pecked my head.
Our moment was broken when "sibling love without me not fair Arnav bhai, you only love your doll" we pulled away to find Mallika standing with a sulked up face shooting daggers at us , bhai chuckled and pulled her towards us "come here you little monster" we all had a group hug and pulled back hearing a fake cough , again someone has to interrupt right.
We pulled away to find a beautiful young lady standing "Mallika dad is calling yo.. Mr. Raizada You..!" The lady tells Mallika and suddenly exclaim loudly as she saw my..bhai? That's weird..and why is bhai grinning like an idiot..okay is it only me or the whole world has gone crazy..? The look on Mallika's face tells me the world has gone crazy .
"Ms. Kapoor nice meeting you here…" bhai said slyly…okay…
"Annie this is Khushi di and di this is my bestie Annika!" Oh so she is Khushi di , last time I saw her I was eight and after that she along with Chandni di went to London for study.
"This is our Annie.. omg kid you grew up so much.." she said hugging me.
"You are Mallika's sister ?" Bhai asked bemused
"yes Arnav bhai that's exactly what I said right now.." mickey told him in a duh tone.
"Ah.yes di this is Annie's big brother Arnav bhai and bhai you know who she is.. so I'm taking Annie with me you guys continue bye.." mickey said grabbing my hand and running away from them , I looked back when I didn't got a response but.. bhai was smiling playfully at Khushi di and she looked embarrassed..? What the hell is happening here?
"Annika…" I stopped hearing his husky voice and my heart beat quickened , I turned back and found him coming towards me.
Mickey smiled at him and left giving me a wink. Was all this planned.. did..he planned all this..?
"Hi.." he spoke looking at me smiling and it did made my heart pound against my ribs so hard that I bet all on the cruise would have heard it.
"Hi.." I spoke meekly getting lost in his eyes…
"you look beautiful…" he spoke after a while.
I lifted my eyes from my shoes to look at him in the eyes with my cheeks being painted pink from his complement
"um thanks.. you look good too.." I spoke shy
" so how come you here?" I asked
"my dad is the business partner of mr. Kapoor and you?"
"The Kapoor's and we are family friends so.."
"Your family..?"
"The Raizadas.." just as I said out those words he had a twinkle in his eyes
"you're the daughter of Anish Singh Raizada..?" He asked amused and I nodded not wanting to say something but, if someone asked me this six months ago I would have told them yes with pride in my heart…
"oh gosh Annika..!" He said excited grabbing my shoulders , my eyes widened and my heart burned at the contact, excitement rushed through me his one gesture made me feel new feelings which I didn't know about.
"I didn't knew I'm friends with my idol's daughter..!" And that wiped my smile.
"Shivaay here you are.!" I looked behind to find mr. Shakti Singh Oberoi walking towards us
" aah Annika nice to see you again" he said smiling down at me which I returned
" same here uncle"
"dad how do you know her?" Shivaay asked him withdrawing his hand from my shoulder which complained at the loss.
" who doesn't know the princess of the Raizada" the endearment was a forbidden topic which hurts today also.
" I have met her several times in the business parties with her badepapa and chachu and not to forget her brothers.." I smiled at him
"I will take my leave uncle"
"sure beta"
"bye Annika"Shivaay smiled
"bye Shivaay" I made my way to my cabin smiling at shivaay's actions , only thinking about it makes my heart racing.
"Sunshine…" and that threw my happiness in hell , I closed my eyes and fisted my hand , a hand grabbed my shoulder.
I opened my eyes and turned around making the hand drop , to face mom.
She looked shocked to see my eyes having no emotion, which resembles my feelings for her absolutely nothing .
"What do you want Mom ?"
She closed her eyes and looked hurt. Wow, nice acting , anybody who would find it real but not me. How many times can you trick someone. I have fallen for her acting enough times but now I won't!.
Precap: Shivaay and fun unlimited and much more…!!!!
Author's note : Hi readers thanks for reading the chapter hope you enjoyed it . I wanted to ask you guys something um.. are you upset with me or I made you angry by any means if I did I'm really sorry . please forgive me for not updating for such long time I was stuck in my family problems otherwise I would never leave you hanging like that .
And I promise you I will update everyday or every other day pinky promise , just don't be angry with me . I miss your comments very much so please try to share your views about the story with me….
until next chapter Adios…….


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Our blog contains short snippets from our full research coverage. We release new content regularly, so check back often for the latest insights.
This blog post examines the different components of the insurance technology (insurtech) ecosystem. We will illustrate what the categories of innovation are and which categories have the most companies. We will also compare the categories in terms of their funding and maturity.
The following infographic summarizes the founding date distribution of Insurance Technology companies to show the percentage of Insurance Technology companies that were founded within a specific time period. It shows that the founding of Insurance Technology companies reached its peak from 2014-Present, with 27% of the companies being founded during this period. Company founding was at the lowest in 2000-2001 and 2002-2003, with only 2% of the companies being founded during those four years. We are currently tracking over 535 companies in 13 categories across 47 countries, with a total of $4.59 Billion in funding. To see the full list of 535 Insurance Technology companies, contact us using the form on www.venturescanner.com.
The following infographic summarizes the average funding for each Insurance Technology category to show which Insurance Technology categories are the best funded. It shows that Product Insurance is in the lead with $63.24 Million funding per company, followed by Auto Insurance with $58.95 Million per company. Please note that this data is based only on Insurance Technology companies that have publicly available funding data. We are currently tracking over 511 companies in 13 categories across 47 countries, with a total of $3.74 Billion in funding. To see the full list of 511 Insurance Technology companies, contact us using the form on www.venturescanner.com.
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'Spanish Girls Are Beautiful' brings the hazy world of women and weed into sharp focus, with the intersection of urban and suburban New York serving as a backdrop to the story of Taylor and Jayla.
An all female ensemble cast throws down a real picture of two would-be lovers living in totally different worlds. Can love cross the county lines that divides the 'hood from the 'burbs?


text: It's vastly approaching the 3-year bracket in which I received my Autism diagnosis back in 2016. Time has truly flown by! I often hear the term "suffering from Autism" which I felt encouraged to shed some light on, as somebody on the complex Autistic spectrum. I understand that the spectrum is large and people enlisted… Continue reading Autism and me: Suffering or growing?


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This Three Amigos Costume was so much fun! I took Black buttondown shirts and shortened them to make the jackets and then free-hand drew on the symbols and squiggles on the arms and legs of the pants (which I used black hospital scrubs for).
I used white puff-paint and silver glitter to make the designs really stand out. We won the contest!


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If you are a lady, you would understand after I say "The Dress have the Power to make or damage". Every vast event in a woman's life is marked with the aid of the get dressed that went together with it. Woman round the sector have closets complete of those attire they just cannot component with.
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