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A couple of weeks ago, I decided to buy my first ever products from Lush after a friend kept recommending a couple to me. I bet you're all staring at the screen in disbelief over the fact that I never ever stepped into the store before that day and lord knows why I never did before. One of my lovely work friends kept telling me to buy 2 products from Lush that she swears by; Angels on Bare Skin Cleanser and Bubblegum Lip Scrub.
I won't lie, the main reason I never rushed into Lush is the strong smell the shop leaks onto the street. Whilst in Harrogate a couple of weeks ago I decided to pop in and pick up the products my friend had suggested. I wasn't completely enamoured by the look of the cleanser and the bubblegum scrub was super bright and strong-smelling. After using these for a couple of days my initial thoughts quickly changed and took a turn for the better.
I was worried that the cleanser would be way to harsh on my skin but by following the instructions on the back, you can't really go wrong. You take a small amount of cleanser in your palm and add water turning it into a paste. This is then massaged into the skin before washing off with warm water. The lip scrub only requires a small amount which is rubbed into your lips and again rinsed away with water. My skin has been looking fab since starting this cleanser, and I can't thank my friend enough for recommending it too me. I feel like I've been missing out all this time! The scrub has done wonders as well as I tend to suffer with dry lips quite a lot especially this time of year. I have been noticing a real improvement and will continue to use this every so often to keep my lips looks smooth and healthy.
Overall I'm amazed by both of these products and will definitely be re-purchasing them in future. I would thoroughly recommend the cleanser to those of you with sensitive skin like myself as it's worked wonders at combatting recent breakouts quickly. If you have never been to Lush before like I hadn't then what are you waiting for?
Laura x


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Barren Earth Issues New North American Road Report
Band Photo: Barren Earth (?)
Finnish progressive death metal act Barren Earth is currently embarked on a North American tour, and you can check out the band's first tour diary here. The group has now checked in with the following new road report:
"The other day back in Louisville, Kentucky, the culinaristic choice of the day was a Chinese restaurant. We were served by a friendly Chinese woman, and the atmosphere was nice. There were, however, problems with communication, since her Chinese accent, combined with a heavy Southern dialect made her speech virtually incomprehensible to us. As a result of this, although me and Marko ordered exactly the same dish, we got two distinctly different portions. But the food was good. The waiter was also in a humorous mode, and was constantly joking. We didn't understand the jokes, but she was laughing, so I'm sure they were funny.
"In Kentucky, the musical reference of the day was obviously Bill Monroe's classic 'Blue Moon of Kentucky'. But we didn't see a blue moon, nor a moon of any other colour. With the absence of gloves, the only thing blue towards the end of the day were our fingers..
"With the cancellation of the Tulsa gig, our route was changed so that we would be spending the day off in Jackson, Mississippi. Although the cancellation was unfortunate, this new bit of routing did provide me with the chance of being able to sing, 'I'm goin' to Jackson…', and that really felt quite cool.
"The day in Ridgewood, Jackson was uneventful. What was notable was the lack of pedestrian paths. It seems that the transport method of choice around here is to drive a car. Many of these places are clearly not designed with the walking citizen in mind. And so our pedestrian group of Finnish rockers was regularly accompanied by honking horns, as we staggered on the busy streets, trying desperately to avoid the menacing Ford pick-up trucks hurdling by.
"In Georgia it was raining, in Kentucky we had snowfall, and now the trilogy is complete, for in Mississippi yesterday we had a shower of hail. Biting and vicious drops of hail were pouring down, as plans of re-christening this tour to 'Hail, Hail, Rock'n roll' emerged.
"It seems that the bad weather conditions are not done with us, or indeed USA, yet. It was bad enough to cancel Tulsa, but now things have turned worse. The bus call was at 3 am, and the plan was to reach the next destination, Austin, Texas, by 4 pm. Waking up at around 8 am, I sensed that the bus was not moving. Suspicions were soon confirmed. We were, and still are, only 8 miles from where we started. The roads everywhere are covered in ice and traffic has come to a halt. It is so unheard of to have icy roads in Mississippi, that no preparations for this situation had been made. And now it has resulted in this. There is no way to reach Austin today, so regrettably that one, also, has now been cancelled, and we are still stuck in Jackson. It's quite cool to sing 'I'm goin' to Jackson'; it's not quite as cool to sing 'I'm stuck in Jackson.'
"This is a major setback, not least because for the whole trip so far, Mikko has been enthusing about a certain restaurant in Austin, namely Stubb's BBQ. We have heard mouth-watering tales of savoury grill dishes, and had been looking forward to dining at Stubb's tonight. Then again, I am not always convinced that Mikko is the man to ask for gastronomic recommendations, given that yesterday his main course for the day was a can of SPAM, eaten raw with a plastic fork, straight out of the tin."
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So Long My Love (American Ideal-Letmedowneasy-Camluck) started from post four and led at every call, posting fractions of :27, :56.1, 1:25 and 1:53.3. She was a neck in front at the wire and the mile clocking represents a new lifetime mark. Completing the race were Up Front Kellie Jo, Sweetheart Dance, Power Everywhere, Riverdancer and Jolting Kate.
Thursday's win was the fifth victory this year, and ninth career score for So Long My Love and it bolstered her lifetime earnings to $54,253. She was an upset winner, returning $27.00 to win.
$30,000 Pick 5 on Monday
Northfield Park is announcing the addition of a $30,000 Pick-5 total pool guarantee to Monday's (May 5) program.
Beginning in Race 3, the $30,000 Pick-5 guaranteed total pool includes a carryover of $5,786.88.
Northfield's Pick 5, Pick 4 and the Pick 3's offer a reduced takeout rate of just 14 percent.
Monday also debuts a new wagering opportunity with the 20-cent Super High 5. A perfect winning ticket requires a bettor to select the first five finishers in order in Northfield's 13th race. The wager offers an attractive reduced takeout rate of just 14 percent and large carryovers of 75 percent of pools when the correct sequence is not selected.
To kick-off the Super High 5, Northfield Park Players Club members will receive a combination package of five FREE Super High 5 wagers and a FREE live program from 5 – 7 p.m.
Monday's post time is 6:00 p.m.
by Ayers Ratliff, for Northfield Park


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Too often, the day to day monotony of routine can stifle inspiration. I visited a certain famous Champagne maker on a once in a lifetime scheduled visit years ago, and unfortunately had caught a virus and lost my voice. He offered the following remedy: mix egg yolks with champagne and drink it down. "Voila, you shall be cured!" This particular Champagne maker has provided more than just health tips; he has inspired my palate. While we can never copy exactly his wines here in Santa Maria, we can certainly learn from him and put our own touch on his style. Therefore, in 2014, Clarissa and I set out to make our own version of a more oxidized and layered sparkling wine, with deep roasted hazelnut, marzipan and a light cloud of whipped cream. Baked apples on the finish round out a truly delectable sparkling wine with extended tirage, and deep red rose petal aromas add an element of sensuality. This, friends, is a special bottle, one that we hope will yield to sighs of satisfaction, special moments, and of course, inspiration. – Laura Booras, General Manager
21 cases produced
pH: 3.16, TA: .99
Picked on August 9, 2014.
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Video Uploader and Publisher for ASP.NET MVC
02 February 2015
We just released latest version of video uploader compatible with asp.net mvc projects. It can be easily integrated in your existing asp.net mvc project to upload unlimited no of videos, publish it one by one, manage published videos in cloud and save information in database .
For complete documentation please visit


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From Tuesday 22 September to 6pm on Saturday 24 October 2020, we will only add content to this website in accordance with Council's Election Period Policy and the Local Government Act 2020. The majority of our services will continue as normal.
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Newark Venture Partners announced today that it closed a $23 million investment fund, part of a $50 million intended fund, to finance seed and series A-stage tech startups.
The CEO of Amazon subsidiary Audible, Don Katz, founded Newark Venture Partners with backing from Audible, Dun & Bradstreet, Prudential Financial, and angel investors.
The recently launched venture firm also announced that it will start accepting applications for its accelerator program, which will be homed in 25,000 square feet of state-of-the-art coworking space in Newark, New Jersey.
Newark Venture Partners Labs, as the program is called, will be working with New York tech entrepreneurs, as well as people from all over the globe, starting September 2016.
NVP will funnel $80,000 into each participating company, and award alumnus that complete "a qualified financing" with up to $1 million in follow-on investment.


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Mitch Farmer June Jam
When: Gates open at 5 p.m.; concert 6-11 p.m. Saturday
Where: Brenau Amphitheater, Prior Street, Gainesville
How much: Tickets are $25 in advance, $30 at the gate; kids 12 and younger are free
More info: 770-654-9323 or www.mitchfarmerjunejam.com
More coverage:
After the death of local Realtor Mitch Farmer, friends, co-workers and family are coming together to continue the June Jam benefit concert he began four years ago.
Farmer began the summer concert in Gainesville to raise money for local organizations and charities. The first year, Farmer booked local favorites April Cummings, King Daddy Polecats and Peggie Hoskins & The Vertigo Band to play the Brenau Amphitheater. The second year was headlined by former "American Idol" star Diana Degarmo.
The third year brought on a whole new set of struggles, including a heart condition that Farmer suffered from. The husband and father died last year.
"Last year Mitch was going to do it, but his health got so bad that it couldn't happen," said Matthew Meeks of Keller Williams Realty Lanier Partners, Farmer and Meeks were business co-owners and partners. "So this year we decided to have it ... to keep the legacy going for something that Mitch started that he believed in so much."
So Meeks organized the concert as Farmer's legacy.
"It's just something that I believe in and something that he believed in," he said. "We are hoping to continue it every year. Mitch was my brother; we were best friends ... we had our sales team at Keller Williams and we were partners for about eight years."
Performing at June Jam this year will be Peggie Hoskins & The Vertigo Band, The Last Minute Band, Allen Nivens Band and King Daddy Polecats.
"We've got some really great charities that we are giving to," Meeks said. "We decided to give money to the Mason Guest House at Emory, and it is a long-term housing facility for family members or people that are going through organ transplants. It gives them an affordable place to stay."
Each band will have about an hour to perform during the benefit.
There will be a silent auction and raffle items including vacations, gym memberships, salon services, fishing trips and art. Part of the money raised from the raffle and silent auction, children's activities and bake sale will go to Farmer's daughter Olivia's trust fund. The Hall-Dawson Court Appointed Special Advocates also will receive part of the proceeds from the event.
Kids games will cost $5, which will include an unlimited play time jump zone, balloons and face painting.
Food will be available from The Varsity and guests are welcome to bring lawn chairs, adult beverages, blankets, picnic baskets and coolers. In case of rain, the concert will move into the gym on the Brenau campus.
"I just want to be able to carry something on in his memory that I know was close to his heart," Meeks said.


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Katie Bozman (PharmD '15)
After four years of study, Marie (Katie) Bozman will earn her Doctor of Pharmacy degree May 29, 2015 as part of the second graduating class of the Philadelphia College of Osteopathic Medicine School of Pharmacy--only minutes from her hometown of Lawrenceville. In fact, she attended both high school and college within just thirty minutes of GA-PCOM's campus.
After learning that a new pharmacy school had opened in Suwanee, Ms. Bozman attended an open house and realized she had found the school she'd been looking for. "Learning more about GA-PCOM, I felt confident that the program's structure would help me to be the well-rounded pharmacist I wanted to be, but more importantly, I was so impressed with the culture of the program. It was obvious even then how invested the program was into its students' successes."
Ms. Bozman explained that she valued how the program allowed her to get involved in activities outside of the classroom. During her first year of pharmacy school, she was able to train as a research assistant with Dr. Eric Wang, Assistant Professor of Pharmaceutical Sciences, and later developed a research project that was awarded first place among pharmacy students in the College's annual Research Day. In her four years at PCOM School of Pharmacy, Ms. Bozman held leadership positions with the American Society of Health-System Pharmacists, the School of Pharmacy Curriculum Committee, and the GA-PCOM Student Ambassador Program. She also authored the School of Pharmacy's student blog, "Confessions of a Student Pharmacist," documenting her journey through pharmacy school.
In March, after interviewing in five states for a pediatric residency position, Ms. Bozman matched with her "dream program" at Children's Healthcare of Atlanta where she will spend a year working with various pediatric populations and in specialties including transplant, neonatology, and oncology. "One aspect I am most excited about is the opportunity to develop my skills in pediatric clinical research," Ms. Bozman said. "Because of the research experiences I was able to develop during my time at GA-PCOM, I strongly believe that I will be able to cultivate and complete a research project that will improve medication safety and patients' quality of life." In the future, Ms. Bozman plans to complete a second year of residency and then seek a specialist position when beginning her pharmacy career.


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Whether collaborating with designers or conceptualizing in-house, the crew at High Plains Drifter takes great pride in bringing the artistic visions of our clients to life. We specialize in custom, high quality wood design and metal fabrication, for both commercial and residential projects. We've been fortunate to work with some of Nashville's most creative entrepreneurs and are humbled to have put our stamp on so many of the city's newest dining establishments. Whether residential, commercial, custom furniture or decor, High Plains Drifter focuses on exceeding our client's expectations with precision, passion and professionalism.
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Structural basis and prediction of substrate specificity in protein serine/threonine kinases
Edited by Susan S. Taylor, University of California at San Diego, La Jolla, CA, and approved November 18, 2002 (received for review July 16, 2002)
The large number of protein kinases makes it impractical to determine their specificities and substrates experimentally. Using the available crystal structures, molecular modeling, and sequence analyses of kinases and substrates, we developed a set of rules governing the binding of a heptapeptide substrate motif (surrounding the phosphorylation site) to the kinase and implemented these rules in a web-interfaced program for automated prediction of optimal substrate peptides, taking only the amino acid sequence of a protein kinase as input. We show the utility of the method by analyzing yeast cell cycle control and DNA damage checkpoint pathways. Our method is the only available predictive method generally applicable for identifying possible substrate proteins for protein serine/threonine kinases and helps in silico construction of signaling pathways. The accuracy of prediction is comparable to the accuracy of data from systematic large-scale experimental approaches.
Posttranslational modification of proteins by phosphorylation is the most abundant type of cellular regulation. It affects essentially every cellular process, including metabolism, growth, differentiation, motility, membrane transport, learning, and memory. Defects in protein kinase function result in a variety of diseases, and kinases are a major target for drug design. To ensure signaling fidelity, kinases must be sufficiently specific and act only on a defined subset of cellular targets. Understanding the basis for this substrate specificity is essential for understanding the role of an individual protein kinase in a particular cellular process.
Experimental approaches for determining specificity, such as the use of oriented peptide libraries (1), are expensive and laborious. Identification of in vivo substrates is even more difficult (2). Although identification of novel putative protein kinases via sequence analysis (3, 4) is straightforward, their biological role cannot be generally predicted, and functional genomics approaches are not yet adequate (5, 6). Substrate identification remains one of the rate-limiting steps in understanding the biological roles of novel protein kinases.
The three-dimensional structures of several protein kinases, some with bound substrates and nucleotides, have been determined (7). All protein kinases show a common fold, consisting of two lobes hinged through a short linker region. The active site, where the phosphoryl group is transferred from ATP to the target residue of the substrate, is located in the cleft between the lobes. Active forms of all protein kinase structures have a similar "closed" conformation, and we reasoned that the available structural information could be exploited to develop computational methods that predict substrate specificities of uncharacterized kinases.
On the basis of an analysis of the crystal structures of peptide complexes of protein Ser/Thr kinases (7–11), we identified 20 enzyme residues ("determinants") that contact the side chains of the residues surrounding the phosphorylation site [only substrate positions (−3), (−2), (−1), (+1), (+2), and (+3) were considered]. Using molecular modeling and sequence analysis of kinases and substrates, we extracted a set of rules that guide the specificity of binding to these positions. We implemented these rules in the web-interfaced program predikin, which performs an automated prediction of optimal substrate peptides by using only the amino acid sequence of the protein kinase as input. To explore the utility of the method, we used predikin to analyze the signaling pathways in two cellular processes in yeast, cell cycle control and DNA damage checkpoints, and predicted new connections in these pathways. Our method should be generally applicable to identifying possible substrate proteins for protein serine/threonine kinases and should aid in unraveling signaling pathways in which these proteins may be involved.
Materials and Methods
Identification of Binding Sites and Sequence Motifs.
The three-dimensional structures of cAMP-dependent protein kinase [protein kinase A (PKA); Protein Data Bank (PDB) ID code 1JBP (9)], phosphorylase kinase [PHK; PDB ID code 2PHK (10)], and cyclin-dependent kinase (CDK) 2 [PDB ID code 1QMZ (11)] with bound substrate peptides were studied to find significant contacts between the catalytic domain and the side chains of the peptide (insightii, Accelrys, San Diego). The locations of key binding residues (determinants) were defined in relation to structural features and conserved sequence motifs (12).
Protein Kinase Models.
As a part of the analysis of substrate specificity determinants, comparative models (insightii) of the kinase catalytic domains with bound substrate peptides were constructed for several kinases [e.g., protein kinase C (PKC)α, PKCζ, PKCμ, calmodulin-dependent kinase (CaMK) 2 (CaMKII), and NIMA]. The choice of template kinase depended on the protein kinase group [AGC (protein kinase A, G, C), CaMK, cyclin-dependent kinase, mitogen-activated protein kinase (MAPK), glycogen synthase kinase 3, CK2 (CMGC), etc. (12)], local similarity, and specific structural features.
The coordinates of ATP or ATP analogues were transferred from the template to the model by superposition. The complexes were energy-minimized using steepest descents and conjugate gradients (discover) to rms deviation of bond lengths of 0.0001 Å, with the coordinates of the backbone atoms of the structurally conserved regions kept constrained. The minimized complexes were examined for likely interactions (<3.5 Å) and steric and electrostatic clashes. The complementarity of an amino acid side chain with a subsite was estimated from the size and charge complementarity and the ability to form favorable interactions (reasonable hydrogen bonds, ionic and van der Waals interactions).
Computer Program.
The program predikin was written with JavaScript. It accepts a protein kinase sequence and outputs predictions of possible heptapeptide substrate sequences. First, it locates (H/Y)RDLKPEN as the characteristic conserved kinase motif and extracts the kinase catalytic domain from the protein sequence provided. Next, it locates other (semi)conserved kinase motifs and, based on the proximity to these motifs, locates the determinant residues. It then applies the specificity rules and predicts an optimal heptapeptide sequence.
To run the program, the user inputs the kinase type and sequence into a form in the browser window. Output consists of the locations of key kinase motifs, the type of kinase, a list of the determinant residues, a list of possible substrate heptapeptide sequences, and commentary text. Substrate data are passed to another window (automatically opened via a link) that contains substrate sequence data formatted for protein database searching.
Searching Protein Databases for Putative Substrates.
Putative substrates were identified using prosite (13), short peptide blast (3), or scansite (14). The searches were usually limited to the same species as the protein kinase. prosite finds all of the sequences containing the permutations of the residues provided. blast will introduce some substitutions and rank the hits according to similarity to the original motif; however, several searches are required to cover all of the sequence choices in a motif. scansite will also introduce substitutions and rank the hits, but only one search is necessary; the simplified user motif method ("Quick Matrix") was used, where residues making multiple contacts with a subsite pocket were considered "primary" residues and other acceptable residues making fewer contacts were considered "secondary" residues. scansite searches were generally limited to no more than 200 hits, or to scores less than 0.12 (usually, ≈20% of known substrates exactly match the primary residues, and ≈40% exactly match the primary and secondary residues). We found scansite to be the most useful method. The comparison of predictions with the known phosphorylation sites in PhosphoBase was performed using the simplified user motif method in scansite, selecting the substrate protein (according to PhosphoBase) by using the species name and the name of the protein.
Results and Discussion
Development of the Procedure for Specificity Prediction.
The information we used to develop our procedure included the amino acid sequences of protein kinases, the available crystal structures of protein kinases [particularly enzyme-substrate complexes of PKA (9), phosphorylase kinase (10), and CDK2 (11)], and the substrate specificities determined by oriented peptide library experiments (1, 15–17).
The rules were developed in several stages: (i) analysis of residues in contact with the side chains of the substrate in relevant crystal structures; such determinant residues were numbered 1–20 starting at the N terminus (Fig. 1, Table 1, and Table 3, which is published as supporting information on the PNAS web site, www.pnas.org); (ii) locations of determinants were defined in protein kinase sequences relative to conserved sequence motifs (12); (iii) correlations between substrate specificity information (oriented peptide library data), and the determinant residues were sought, taking into account complementarity with the substrate in terms of size, polarity, charge, and hydrogen-bonding potential.
The prediction methodology relies on several postulates supported by our analyses and available data: (i) all protein Ser/Thr kinases adopt a similar fold with little scope for conformational differences in the binding cleft, and bind substrates in a similar extended conformation with few bridging water molecules involved (supported by the available crystal structures and the proximity of the substrate binding residues to conserved sequence motifs and structural elements); (ii) residues at the analogous position in a subsite pocket bind the same substrate residue(s) (supported by the available crystal structures); (iii) determinant residues can be identified in any kinase sequence by locating the conserved structural motifs (supported by sequence analyses).
Not all determinants affect specificity, or are involved in substrate binding in all kinases (9–11). The correlations between the determinant residues and substrate specificity were extrapolated to cover other possible sequence variations (Table 4, which is published as supporting information on the PNAS web site). The use of structural data (experimental and comparative models of kinases) implies that that the developed rules are only partially empirical.
General Descriptions of Individual Subsites.
(−3) subsite (determinants 6–8, 10, and 20).
The specificity at (−3) primarily depends on determinant 6. For kinases in the AGC and CaMK groups, Glu or Asp in this position results in a preference for Arg or Lys at (−3). Determinant 7, when hydrophobic, generates the specificity for Pro or Met at (−3). CMGC kinases have residues other than Glu or Asp at 6 and are less specific; determinant 10 consequently has a bigger influence on specificity [e.g., 10 His indicates a (−3) preference for Glu in CK2 (11)].
(−2) subsite (determinants 10, 14–17, and 19).
Binding in the (−2) pocket of AGC and CaMK kinases primarily involves determinant 17 [Phe, effecting hydrophobic specificity; or Tyr, with the phenolic and phenyl groups both possible ligands (for Gln/Arg/Lys and Met/Phe, respectively)]. This specificity is modified in a predictable manner by the nature of 16 [ranging from Glu in PKA (Arg specificity) to Gly in CaMKII (broad specificity)]. Determinant 17 is Trp in CMGC kinases and occludes much of the (−2) pocket, so that binding is restricted to Val, Pro, or Gly; the exception is CK2, where 15 Arg causes a preference for Glu at (−2).
(−1) subsite (determinants 1 and 2).
The (−1) residue makes few contacts with the enzyme. A small residue such as Ala, Gly, or Pro is often found at (−1), although its side chain makes no contacts with the kinase.
(+1) subsite (determinants 11, 13, 15, and 18).
AGC and CaMK kinases frequently prefer large hydrophobic residues, e.g., Phe, when 11, 13, and 18 are large hydrophobic residues, and 15 is Pro (they all contact the phenyl ring). Variations altering specificity include hydrophilic residues at 11, smaller residues at 13 and 18, and Leu or Ala at 15. Tyr at 18 (titin) or at 13 (NIMA) indicate a preference for (+1) Arg. In CDKs and mitogen-activated protein kinases (MAPKs), 15 Leu is diagnostic of the obligatory Pro specificity at (+1).
(+2) subsite (determinants 2, 3, 4, 5, and 11).
In some cases, the binding involves charge–charge interactions, and the specificity is well defined [e.g., PKCα (for Arg/Lys), CK2 (for Glu/Asp)]. Other combinations can result in broad specificity (e.g., PKA, phosphorylase kinase). Determinants 4 and 5 do not bind substrates in CDKs, because this region is involved in cyclin binding (11).
(+3) subsite (determinants 12 and 13).
The subsite is rarely very specific; exceptions include PKCα and CK2 (18). Determinant 12 is often phosphorylated and can result in a preference for Arg/Lys. Determinant 13 (if large enough) is shared with the (+1) site.
No reliable structural information is currently available for subsites N-terminal to (−3) and C-terminal to (+3), although specificity occasionally extends outside this region. It is common that more than one amino acid can successfully bind in a subsite; for example, a typical (+1) subsite can accommodate residues such as Phe, Leu, Met, Ile, and Val to a similar extent. Sometimes even side chains with different properties (e.g., hydrophobic and hydrophilic groups) can bind with similar probabilities. Because the specificity at one subsite can depend on other subsites [links are observed between the (−3) and (−1), (−1), and (+2), (−2) and (+1), and (+1) and (+3) subsites], conditional rules are necessary. Determinants shared by two subsites can contribute to either subsite, and in some cases, both.
Our methodology does not currently cover Tyr kinases; examination of Tyr kinase structures shows that the (−1) residue in the bound peptide bypasses the (−1) and (−2) pockets and binds to what normally is the (−3) pocket, therefore different rules apply to these enzymes. Our rules can be used for protein kinases other than AGC, CaMK, and CMGC groups (e.g., dual specificity kinases, CK1, prokaryote protein kinases); however, the results are less reliable, because little structural information is available.
Computer Program.
We incorporated the rules described above into the web-based computer program predikin, which uses only sequences of protein kinases as input (available on www.biosci.uq.edu.au/kinsub/home.htm; functional within INTERNET EXPLORER 5). The user inputs the amino acid sequence of a protein, and predikin extracts the sequence of the protein kinase catalytic domain (if present), locates the sequence motifs, identifies the determinant residues, and writes a prediction for subsites (−3) to (+3) with relevant comments. A search for possible target proteins by using the predicted optimal heptapeptide sequence can be carried out with prosite (13), blast (3), or scansite (14).
Accuracy of Predictions.
The substrate sequences predicted by predikin are optimal and analogous to those generated by an oriented peptide library experiment. The predictions agree well with the peptide library results (Table 1).
Peptide library data previously suggested that a complete complement of possible interactions is unnecessary for productive substrate binding; this observation has ramifications for using an optimal motif for substrate searches in protein databases (1, 14–17, 19). In the example of PKA [hydrophobic residues are predicted for (+1), (+2), and (+3)], the optimal motif is likely to be unsuitable as a protein kinase substrate, because it would be too hydrophobic to be found on the surface of the protein. Small residues, such as Gly, Ala, Ser, and Pro, are frequently found in PKA substrates at positions (−1), (+1), (+2), and (+3), even though in most cases they would make few contacts with the enzyme; they appear beneficial by reducing hydrophobicity and increasing the likelihood of location in a flexible loop. PKA consequently displays a broad specificity, and the substrates are difficult to predict based on searches with the optimal motif.
Substrate searches based on peptide library data (14) showed up to 70% of published sites [PhosphoBase (20)] predicted at the top 5% level. We performed an analogous test based on predikin predictions (see Materials and Methods) and found comparable statistics on sensitivity and specificity for protein kinases as diverse as PKCα, CaMKII, CK1, and CK2. The substrates not found by either method appear suboptimal, poorly matching the motifs, and many may not be functional in vivo. The accuracy is comparable to secondary structure predictions, as well as systematic large-scale experimental methods (21–24). In individual cases, we observe that the predikin predictions can resemble real substrates better than the peptide library results do (e.g., Slk1, Chk2, and CK1; Table 1), and scansite searches using our predicted motif can produce better results than the equivalent searches using experimental peptide library matrices (14) [e.g., in the first 100 hits, our predicted motif finds 3 and 10 known substrates, and peptide library-based motif only 2 and 1 known substrates for PKCα (16) and CK2 (18), respectively]. Analyses show different predictive powers for individual subsites of the AGC and CaMK class kinases; 70% for (−3) and (−2), 55% for (+1), and 40% for (+2) and (+3) [these numbers are based on direct comparisons of predicted residues to the ones found in real substrates of Ser/Thr kinases (18); no predictions were generally made for the (−1) site]. The predictive power also depends on the specificity; (−3) and (−2) predictions of Arg specificity appear particularly reliable, likely due to the larger number of interactions the Arg residue makes with the protein kinase at these subsites.
predikin can locate phosphorylation sites in a single protein sequence with high reliability. However, the key utility of predikin is to be able to predict novel putative substrates through searching protein databases. The number of hits can range from 1 to 600 for different kinases (using prosite and a single organism; it is most commonly between 100 and 200). The user has the flexibility to restrict the degeneracy of the profile if too many hits are obtained, but the probability of finding known positives increases with the number of hits considered. Only ≈20% of hits appear unlikely as substrates due to incorrect cellular localization or other considerations. These results indicate that predikin can be used even for genome-wide analyses, especially if some additional filtering tools are applied (14). However, any searches have to be performed prudently, and the following caveats need to be considered: (i) some protein kinases are inherently not very specific for reasons outlined earlier; in the cell, apparently weak kinase specificity may be boosted by anchoring, adaptor and scaffold proteins, e.g., the A kinase anchor proteins for PKA (25); (ii) the specificity of some kinases depends on subsites outside the (−3) to (+3) range; (iii) determinant residues can be phosphorylated, affecting specificity (e.g., determinant 12 is a phospho-threonine in many protein kinases); (iv) substrates can be prephosphorylated in adjacent sites [e.g., many phosphorylation sites in CK1 substrates are surrounded by phospho-serines (18)]; (v) small residues such as Gly, Ala, Ser, and Pro are often present in substrate sequences even though they make few, if any, contacts with the enzyme; residues binding in other sites appear to be able to provide adequate interactions for the substrate to bind successfully; (vi) occasionally, residues inconsistent with our predictions (or peptide library results) are found at (+3), together with small bend-forming residues at (+2) [e.g., protein kinase G (PKG) and CaMKII]; because the amino acids present at (+3) appear to follow the predictions for (+2) instead, the (+3) residues may in these cases in fact bind to the (+2) pocket; and (vii) the substrate sequence must be accessible to the kinase and capable of adopting an extended conformation.
To test the utility of predikin in finding novel putative substrates and constructing signal transduction pathways, we analyzed the protein kinases involved in the cell cycle control and DNA damage checkpoint pathways in yeast. We identified phosphorylation sites for substrates with unmapped sites and many plausible phosphorylation events within the pathways and between proteins known to interact (Table 2; Figs. 2 and 3; Tables 5 and 6, which are published as supporting information on the PNAS web site).
Yeast Cell Cycle Control Pathways.
Under normal circumstances, yeast cells passage through G1, S, G2, and M (mitosis) phases of the cell cycle. Many of these processes are controlled by a single "master switch" protein kinase CDC28, a CDK that uses different cyclins at different stages of the cell cycle (26). For example, the transition from G2 to M and the simultaneous septin ring formation requires the complex of CDC28 with cyclin B (27). A group of septin-localized protein kinases controls CDC28 activity; the dual specificity kinase Swe1 is a negative regulator (antimitotic; phosphorylates and inhibits CDC28), whereas KCC4, Gin4, Hsl1, Cla4, and Elm1 are positive regulators (by inhibiting Swe1 activity). The cell cycle is shut down in situations such as pheromone-induced cell cycle arrest (leading to mating), high osmolarity, and starvation (leading to filamentous growth); signaling in each of these situations involves a central MAPK cascade with a shared MAPKKKK (Ste20) and MAPKKK (Ste11), before branching to a specific MAPK (e.g., Kss1 in filamentous growth, Fus3 in mating) (26). The known pathway connections are depicted with solid lines in Fig. 2. The results of predictions for selected cell cycle control kinases are listed below.
CDC28 is predicted to have a broad specificity, consistent with its many known substrates and diverse roles (not all known substrates have functions related to cell budding). Our analysis predicts phosphorylation sites in several proteins known to be substrates (with hitherto unknown sites), and several new substrates are predicted, suggesting regulatory connections to various related pathways such as pheromone-induced cell cycle arrest and mitotic exit network (e.g., CDC7, Elm1, and Cla4; Fig. 2).
Our analysis confirms CDC28 as Swe1 substrate (27) and predicts new substrates, including CDC7 and Rck2 in related pathways, and CDKs Ctk1 and Kin28. Phosphorylation of Rck2, a Hog1 substrate in the antimitotic high osmolarity pathway (26), would, for example, be predicted to activate the kinase.
KCC4, Gin4, and Hsl1.
These closely related kinases are predicted to have a similar specificity, except that Hsl1 differs at (+2). We predict that KCC4, Gin4, and Hsl1 phosphorylate Swe1 at a site that would interfere with ATP and substrate binding. The predicted site in CDC28, on the other hand, is close to the cyclin-binding site. An analogous site is present in Sgv1, involved in recovery from pheromone-induced cell arrest (28). We identified two other potential KCC4/Gin4 substrates that would link the various cell cycle control pathways, CDC15 (29) and Ste7 (18). Hsl1 is known to bind and phosphorylate Hsl7, the complex then phosphorylating Swe1; we can predict the likely sites in these proteins. Other predicted Hsl1 substrates could contribute to promoting mitosis and suppressing the MAPK pathways.
Cla4 and Ste20.
These two enzymes are predicted to have the same specificity; the opposing functions (Cla4 inhibits Swe1 and Ste20 activates Ste11) must therefore result from different cellular localization and regulation. The major predicted site in Swe1 is shared with KCC4/Gin4/Hsl1. Predictions confirm Ste11 as Ste20 substrate, leading into a MAPK cascade.
Yeast DNA Damage Checkpoint Pathways.
Damage to double-stranded DNA triggers a number of cellular events including cessation of cell cycle and mitosis, apoptosis, and DNA repair. Protein kinases play a central role, both as sensors of damage (lipid kinase-like kinases Mec1 and Tel1 in yeast, ATM, and ATR in humans) and downstream signal transducers (CaMK group kinases Chk1, Rad53, and Dun1 in yeast; Chk1 and Chk2 in humans) (31). Although many components have been identified in checkpoint and DNA repair pathways, only a few kinase substrates have been established. The known pathway connections are depicted with solid lines in Fig. 3. The results of predictions for selected checkpoint kinases are listed below.
Yeast Chk1 is predicted to have similar specificity as its human namesake (19). Several phosphorylation sites are predicted in the known substrate Pds1 [phosphorylation of Pds1 leads to its ubiquitination and destruction by the anaphase promoting complex (APC) (32)]. Other predicted substrates with related functions (33) include the sensor protein kinase Tel1, the checkpoint protein Mec3 (32), and DNA repair protein Rad54 (34).
The predicted specificity of Rad53 is similar but not identical to Chk1. No phosphorylation sites are predicted in its known binding partner Rad9. New predicted substrates include Xrs2 (35) and Rad50 [involved in double-strand DNA break repair and telomeric function through association with Mre11 (36)]; several proteins associated with radiation damage (34); DNA polymerase Rev1 (37); checkpoint kinase Bub1 (38); Rad53-associated protein Smc3 [involved in chromatin adhesion and DNA recombination (39)]; and Dun1 [previously proposed to act downstream of Rad53 (40)].
Several proteins known to associate with Dun1 (23) are predicted to be its substrates; Mre11 and Rad50 [members of the Mre11-Rad50-Xrs2 complex (36)], and Rad9 and Rad24 (involved in nucleotide excision repair and S-phase regulation). Sites are predicted in the known substrates Rfx1 (Crt1) (41) and Adr1 (40). The site in the known substrate Rir1 is successfully predicted, although the (+1) residue is suboptimal (35).
Using the available three-dimensional structural information, we developed a set of rules that govern substrate specificity of classical protein Ser/Thr kinases, CMGC, and dual specificity kinases, and incorporated these rules in a web-interfaced computer program predikin for specificity prediction. Our analyses show that families of kinases with overall sequence similarities or similar regulatory mechanisms do not necessarily have similar substrate specificities, therefore one should be careful inferring functions based on sequence comparisons. Our results suggest that it is possible to make rational predictions of the optimal substrates based on protein kinase sequence alone. We show in an example involving yeast signal transduction pathways that such methodology aids in identifying the substrates of known and novel protein kinases deduced from genome sequences, the components of signaling networks, and therefore has the potential of identifying new therapeutic targets.
The important distinctive feature of our method is that it can make predictions of specificity. Methods exist to suggest sites in proteins that may be phosphorylated by characterized kinases, based on analyses of known substrates (42) or peptide library results (14). Our tests show that our method shows similar accuracy of identifying such sites, but additionally it can predict sites phosphorylated by uncharacterized protein kinases for which no information other than amino acid sequence is available.
Flawless substrate prediction using our methodology is not achievable. Substrate recognition in the cell depends not only on the internal molecular specificity of a protein kinase for a certain peptide sequence, but on other cellular mechanisms, particularly specific localization. To increase the probability of correct identification of substrates, specificity information should be integrated with other available information such as cellular localization, functional information and structural information for substrate proteins, and used with filtering tools such as dual motif searches (14). The significance of the method is its utility. The method works extremely well predicting a phosphorylation site in a protein known to be phosphorylated. The more general utility is the identification of new substrates; even by partially narrowing the list of candidates to be tested experimentally, substantial savings can be made on cost and duration of experimental research.
Our results also show the potential that similar methodology is extended to other proteins which recognize short amino acid motifs, such as modular signal transduction domains (SH2, FHA).
Early work on this project was supported by the Australian Research Council (B.K.). B.K. is a Wellcome Senior Research Fellow in Medical Science in Australia.
↵* To whom correspondence should be addressed. E-mail: b.kobe{at}mailbox.uq.edu.au.
This paper was submitted directly (Track II) to the PNAS office.
- protein kinase A;
- cyclin-dependent kinase;
- mitogen-activated protein kinase;
- CDK, MAPK, glycogen synthase kinase 3, CK2
- Received July 16, 2002.
- Copyright © 2003, The National Academy of Sciences
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- Hardie D G
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- Altschul S F,
- Madden T L,
- Schaffer A A,
- Zhang J,
- Zhang Z,
- Miller W,
- Lipman D J
- ↵
- Ponting C P,
- Schultz J,
- Milpetz F,
- Bork P
- ↵
- ↵
- ↵
- Knighton D R,
- Zheng J,
- Ten Eyck L F,
- Ashford V A,
- Xuong N-H,
- Taylor S S,
- Sowadski J M
- ↵
- ↵
- ↵
- ↵
- ↵
- Hofmann K,
- Bucher P,
- Falquet L,
- Bairoch A
- ↵
- ↵
- Songyang Z,
- Lu K P,
- Kwon Y T,
- Tsai L H,
- Filhol O,
- Cochet C,
- Brickey D A,
- Soderling T R,
- Bartleson C,
- Graves D J,
- et al.
- ↵
- Nishikawa K,
- Toker A,
- Johannes F J,
- Songyang Z,
- Cantley L C
- ↵
- Obata T,
- Yaffe M B,
- Leparc G G,
- Piro E T,
- Maegawa H,
- Kashiwagi A,
- Kikkawa R,
- Cantley L C
- ↵
- ↵
- O'Neill T,
- Giarratani L,
- Chen P,
- Iyer L,
- Lee C H,
- Bobiak M,
- Kanai F,
- Zhou B B,
- Chung J H,
- Rathbun G A
- ↵
- Kreegipuu A,
- Blom N,
- Brunak S
- ↵
- ↵
- ↵
- Ito T,
- Chiba T,
- Ozawa R,
- Yoshida M,
- Hattori M,
- Sakaki Y
- ↵
- Pawson T,
- Scott J D
- ↵
- ↵
- Mendenhall M D,
- Hodge A E
- ↵
- Cid V J,
- Shulewitz M J,
- McDonald K L,
- Thorner J
- ↵
- Sreenivasan A,
- Kellogg D
- ↵
- Edgington N P,
- Blacketer M J,
- Bierwagen T A,
- Myers A M
- ↵
- ↵
- Wang H,
- Liu D,
- Wang Y,
- Qin J,
- Elledge S J
- ↵
- ↵
- ↵
- Zhao X,
- Rothstein R
- ↵
- ↵
- Haracska L,
- Prakash S,
- Prakash L
- ↵
- Roberts B T,
- Farr K A,
- Hoyt M A
- ↵
- Kim S T,
- Xu B,
- Kastan M B
- ↵
- Sanchez Y,
- Bachant J,
- Wang H,
- Hu F,
- Liu D,
- Tetzlaff M,
- Elledge S J
- ↵
- ↵
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- Philips J,
- Herskowitz I


text: Environmental issues such as climate change, pollution and environmental degradation are harmful effects of human activity. Research shows that humanity is living unsustainably and that a collective effort is needed to use Earth's resources at a rate at which they can replenished. You can play an important role by organising SocialErasmus activities that address environmental issues. Explore the following ideas and contribute to a Green world.


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This RP thread is open to anyone, with some stipulation: the astral plane is only accessible to those possessing psychic or mystical abilities (telepaths, arcanists, gods, etc.). There are some exceptions to the rule; anyone can be brought here by an astral "guide" who has the requisite abilities, and it is possible that characters of sufficient scientific acumen could devise some technological means of arrival (perhaps not even on purpose).
The astral plane is a non-physical plane that overlaps the material plane (what most recognize as "reality"). It is a realm of thoughts, dreams, and visions. The landscape (if it can be called such) is unstable and surreal, though a sufficiently strong will can shape it. This can lead to interesting battles in which two powerful minds attempt to shape their surroundings, creating a sort of environmental war.
The most basic rule on the astral plane is that "thoughts are things." A strong mind can create practically anything: will yourself to be holding a sword, and it shall appear in your hand. A character that is weak and sickly on the physical plane may appear strong and healthy on the astral; one's appearance is determined by one's mind, although such forms generally resemble their material forms in some way.
A character's astral form is constantly connected to their material body by a thin, nearly invisible silver cord. Should their astral form be "killed," this cord will yank them back into their biological body, unharmed, but suffering effects similar to a severe hangover. Such individuals will need a recovery period (varies according to their mental fortitude) before returning to the astral plane. Though it is borderline impossible, this silver cord can be severed, breaking the connection between a character's mind and body. The body will fall into a comatose state, and may very well die, if it is not attended to appropriately. The mind will become trapped on the astral plane, and will likely require aid in returning to the body.
In short, the astral plane is a playing field where those with the right abilities can exercise tremendous creativity and wield vast power, but be warned: the plane has its share of both guardians and predators, and the character who causes too much upheaval or draws too much attention will likely encounter them…


text:
Aboriginal People and Canadian Genocide
This is a note to inform you that a Googlevideo version of UNREPENTANT is available. For those of you unaware of this prize winning film, it is an hour and a half documentary about the murder of 50,000 or more Aboriginal children in the residential schools, the attempts to cover up these crimes and the attacks upon the courageous few who have spoken up. Please copy this video and distribute it far and wide. Let the world know the truth about Canada's genocide! See:


text:
Grow or Die!Filed Under: Labels: entrepreneurship, minority business development, strategic planning, technology
It is a fact of nature that healthy things grow. As human beings we understand that infants grow into toddlers into children into teens into adults, and so on. We identify the transition from one stage to another as an indicator of growth. In fact, we expect these growth stages to happen. And when they don't happen or are delayed, we suspect something is wrong and we start investigating, whether through our own research or going to a doctor.
Of course for a human to grow, the individual systems that comprise our bodies, such as the skeletal, immune, digestive, and nervous systems, have to function properly. If any one of these systems is out of sync or degenerative, we will be unhealthy and our growth could be stunted or we could die.
Systems are also the foundation of every business, large or small. These systems, which could also be called operating processes and procedures (written or just understood), allow the business to function effectively (accomplish goals and make money) and efficiently (use as few resources as possible). When any of these systems are dysfunctional or non-existent, the business will be unhealthy and eventually could die (i.e. bankruptcy, liquidation, acquisition).
Good but not Growing
While it's great the number of minority owned firms has increased exponentially over the past 20 years, the focus now needs to shift to growing exponentially more healthy businesses. As I reviewed the recent small business growth statistics, I was astounded to see the high percentage of minority owned firms that earn less than $50,000 per year (see table below). Amazingly, 85.9% of black owned, 76.8% of Hispanic owned, 78.5% of Native American owned firms fall below the $50,000 revenue threshold.
Healthy Systems are Required for Healthy Growth
Let's take a quick look at some of the essential systems that need to be in place for a business to be healthy and positioned for growth:
- Strategic Planning - The on-going, ever-evolving process of structuring the business internally and positioning the business externally to take advantage of current and future opportunities. This includes managing your business according to your long-term vision, mission and values.
- People & Culture - People are the most important asset of any company. How are you creating a culture in your business that values, develops and celebrates people? Whether you have 1 employee or 100, your culture should be inviting and empowering. This also relates to how you engage suppliers, customers and business partners.
- Products & Services - Utilitize creativity and innovation to keep your products aligned with your customers' desires and expectations. You must be able to offer attractive products/services that agree with what your target customers are willing to pay.
- Customer Relations - Without customers you don't have a business. It is essential that you have a strategic and tactical approach outlined for acquiring, serving, satisfying, retaining and rewarding your customers.
- Financial Management - For a small business "cash is king". Strategically manage your finances in order to maintain a strong financial position that meets currents needs and positions your business for future growth.
- Marketing & Advertising - Your company's brand communicates your value to customers. It becomes the launching pad for growth opportunities. Building a strong brand within your target customer market is crucial to growing your company. If potential customers don't understand the value that you provide, then you won't be in business very long.
- Technology - Don't use new (or old) technology just for technology's sake. Leverage technology to reduce costs, improve products/service, increase productivity, and enhance customer interactions.
Many people refer to their business as their "baby" because they have grown it from it's infancy. As I mentioned earlier, when the human growth stages aren't happening in our children as expected, we take them to see a doctor. It's interesting that with our children, although we know them better than anyone else we still go to a baby specialist, i.e. doctor, when something is wrong.
It's unfortunate that many entrepreneurs don't do the same with their businesses. Whether out of pride, ignorance or unwillingness to spend the money (invest in their own well-being), they often avoid having a qualified specialist come in and assess their business condition/situation so they can get a prescription for healthy growth.
At some point, every business needs a check-up with a business doctor, because no business - large or small - can stay in a stagnant, unhealthy position for very long. Either you're advancing and growing or declining and dying. And with the way that business and the world operates today, you can't be satisfied with what you have accomplished up until today, because that may not be enough to survive for tomorrow. So, what are you doing to grow your business?
Empowering Champions,
Paul Wilson, Jr.
1) Mirror Mirror
2) Suggestions for Small Biz
3) The 7 Irrefutable Rules of Small Business Growth by Steven S. Little
Links to this post


text:
This is the HYACINTH quarter length sleeve criss cross top– MADE TO ORDER- in your custom sizing and choice of fiber reactive dye color. NOT HERBAL DYE.
This top is only available in FIBER REACTIVE DYE until further notice.
1. please READ my sizing chart. all sizing charts are different from brand to brand, and I will not start your custom order until I have a confirmation from you that you have read it, and chose your size wisely.
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3. remember, I CANNOT EXCHANGE PANTIES, I CAN EXCHANGE BRAS AND OTHER CLOTHING GARMENTS, BUT SPECIFICALLY I HAVE HAD ISSUES WITH PEOPLE RETURNING UNDIES THAT 'DONT FIT' THAT HAVE OBVIOUSLY BEEN WORN. why someone would ever do this sort of thing, is beyond my understanding… but, please take into consideration the hygienic reasons why I cannot exchange panties if they do not fit.
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**This top is made with the intention to be an everyday staple- and features quarter length sleeves and is designed with the breast feeding mamas in mind ! The criss cross front is there for easy access for mamas and babies. Made with medium weight hemp and cotton 5 way stretch, this top is extremely comfortable and is made to have a snug fit- if you want this top to be a little looser, choose a size bigger ! KEEP IN MIND, THIS TOP IS IN NO WAY ONLY FOR BREAST FEEDING- THIS TOP LOOKS AWESOME NO MATTER YOUR STATUS AS A MOM.
**CALICO ALCHEMY::How these are Made::
ALL GARMENTS ARE – Designed, Hand Cut, Sewn, and Dyed in Northern CA, by Kelley Bennett
FOR NOW THESE DESIGNS ARE ONLY AVAILABLE IN FIBER REACTIVE DYE. I will be offering them in herbal dye, once I am able to test and decide which colors are most economical to offer in each design.
We ask that you take good care of your garments from us especially in the washing process:
1. Machine wash on cold
2. Wash with other delicates
3. AIR DRY FOR BEST AND LONGEST LASTING COLOR and FABRIC ELASTICITY RESULTS !
*you can dry your clothes in the machine, but I do not recommend it for the majority of the times you wash your hand dyed garments
*choose a medium heat or tumble dry.
4. BEST TO OPT FOR A NATURAL LANDRY DETERGENT! for the earth, for us all !
With Love and Gratitude~ Kelley Bennett~ CALICO ALCHEMY ORGANICS
a production of: FAMILY OF LIGHT DESIGNS


text:
Click through to view benefits organizations can gain from mobilizing their workflow applications process.
Every company has business processes that keep the work flowing forward, and many companies rely on workflow software to standardize common activities across the entire organization. Workflow applications have proven extremely beneficial in automating routine tasks such as approvals for vacations, purchase orders and timesheets. In retail, manufacturing, delivery/transport and other niche industries, workflow applications are incredible time savers and often reduce human errors.
A standard rule of thumb is that any task that involves three or more people takes seven business days to complete. When one of those people is out of the office, that completion timeframe can move closer to 10 days. Mobilizing workflow applications keeps business activities moving forward, potentially decreasing task completion times from seven days to seven minutes.
Mobilizing workflow takes away the hassle of e-mail notifications, searching for Wi-Fi connections, accessing the VPN, then logging into a system to approve. New mobile workflow capabilities eliminate those multiple steps. Recipients need only to open the workflow notification on their smartphone, and all the necessary information is available to approve or decline.
According to Willie Jow, vice president for business operations and mobility product marketing for Sybase, mobilizing internal business processes is a significant first step toward creating a truly mobile environment. This type of mobility leads to business process reengineering that can spread throughout the enterprise and lead to new, long-term strategies.
This slideshow highlights the benefits organizations can gain from mobilizing their workflow applications process.
Why IT Projects Fail Recognize the early warning signs of imminent project failure.
10 Top Google Apps for Project Management 10 of the highest-rated apps for project management from the Google Apps Marketplace.
Top Five Rules for E-mail Etiquette Follow these simple rules to help you avoid looking like an amateur in the world of e-business communication.
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text:
We are a UK Battery Repair Service for electric toothbrushes* based in Essex and are currently continuing to offer our professional repair service for a special price of £19.99 (normally £24.99).
Unfortunately electric toothbrushes do not have an eternal battery life. After a number of charge cycles (and usually after the warranty has run out) the battery starts to become less powerful and keeps its charge for a shorter period of time. Often this ends in the charge not even being enough for a 2 minute brush of your teeth.
Manufacturers show their 'generosity' by offering to either service the handset or exchange it for usually well over £50 or people often go straight down the route of binning their old brush (which besides the battery is working perfectly fine still) and purchasing a completely new brush which can cost in excess of £100 for top of the range models.
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Travel for free with BuddyTraveller.com
In today's world there are many innovative ways to travel. It is easier and cheaper than ever to get around anywhere in the world, than at other time in history if you know where to look.
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BuddyTraveller.com is not just limited to the United States. You also have the chance to make a whole new group of friends all around the world! Through this website you have the opportunity to be hosted all around the world!
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Not only can you save money by using this website to enjoy your trip more thoroughly, but will also leave will friends all over the world.
This website also gives hosts the option to charge for room and board. If you want to rent out your couch or spare bedroom to travelers for money on the side this is a great website for you.
You will probably find that the hosts that are charging for your stay have more lavish accommodations. So for those of you who like to travel in style, this is a great alternative to spending a lot of money on hotel rooms.
This is one of the major differences between this website and other websites offering similar services. This allows the hosts the option to make money if
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It is great to travel and see the world. Too many people today think that it is impossible or too expensive for them to do. www.BuddyTraveller.com makes
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There is an entire community of travelers and hosts out there ready to see the world. It is time that you joined them!


text:
Opened 7 years ago
Closed 6 years ago
#1818 closed bug (fixed (in master))
Examining fails after a lack of item to identify
|Reported by:
|Owned by:
If you try to use identify and get the "You have nothing to identify" message, immediate use of the inspect command gives the "You have nothing to examine" message.
Change History (4)
comment:1 Changed 6 years ago by magnate
Very arcane indeed. This does NOT happen if you get any subsequent message after "You have nothing to identify" - such as "You re-order some items in your pack." or even "Welcome to level 2."
comment:2 Changed 6 years ago by magnate
- Status changed from new to confirmed
comment:3 Changed 6 years ago by noz
It happens because the item_tester_hook remains set to item_tester_unknown. Obviously there are several things that clear that hook, but doing an examine straight after an unsuccessful identify doesn't. Can't see yet where to fix it, but will keep looking.
comment:4 Changed 6 years ago by takkaria
- Resolution set to fixed (in master)
- Status changed from confirmed to closed
Fixed with pullreq https://github.com/angband/angband/pull/371


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Words for Delivery
|Mekkatorque's Speech (Provided)
DescriptionAll right, soldier. Time to prove your worth!
Take the speech and board the flying machine "Thunderflash". It's the fastest ship to High Tinker Mekkatorque and this speech is the only thing he's waiting on before starting Operation: Gnomeregan.
Go! Now!
RewardsYou will receive:
GainsUpon completion of this quest you will gain:
Please keep the following in mind when posting a comment:
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Simply type the URL of the video in the form below.
The Wowhead Client is a little application we use to keep our database up to date, and to provide you with some nifty extra functionality on the website!
It serves 2 main purposes:
- It maintains a WoW addon called the Wowhead Looter, which collects data as you play the game!
- It uploads the collected data to Wowhead in order to keep the database up-to-date!
You can also use it to keep track of your completed quests, recipes, mounts, companion pets, and titles!
So, what are you waiting for? Download the client and get started.


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Experiencing Love – Online Course
This is a free course is for anyone who wants to experience more love and kindness in their life.
It began as the realization that there are many people who might never have experienced true, unconditional love – the kind of love that is always compassionate, never judgemental and just loves every part of you.
In my teachings of tantra, 100% kindness is the first thing that we must accomplish in our relationships if we are ever to experience true connection with ourselves, others, or God. But I found that we all defined kindness differently – in the same way that we all define love differently. And so, this series continues the discussion of what kindness is, and what it isn't.
The goal of this 9-video series is to shift what we believe love and kindness to be, so that whether we have ever experienced either in our lives ever before, we can start today and find both. We CAN experience kindness in the world and we CAN experience true love – with our children, friends, and partner – perhaps for the first time.
You can watch the series here or visit my Youtube Channel HERE.
Experiencing Love Series
Going Deeper
Along with this video series, if you would like to go deeper into any of the topics described, I highly recommend reading both of my books – especially if you would like to strengthen your intuition and how to cultivate the levels of love that we discuss here.
Both books are available everywhere online in paperback, ebook and audiobook!


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Roses for Don Jose's Birthday
Last Sunday was a day of celebration in our town and for my host family. It was the Feast Day of San Dionisius who is the patron saint of the town, the Catholic Church, and of Don Jose. It was also Don Jose's birthday. There were many events to celebrate the auspicious day, including a family birthday celebration, Mass, first communion for a few children, a procession for San Dionisius, and a big fair that featured many bands in the plaza.
Can you see the rose petals encased in the netting of her communion dress?
This photo of Don Jose was taken a couple of days earlier when he was offering me a lemonade. He and his wife, Doña Irma, are such lovely people with a great sense of humor.
Doña Irma prepared a beautiful meal for Don Jose, family and friends. After his birthday celebration, we returned to the church in the afternoon to see the procession of San Dionisius. His statue was carried on a very large platform by about ten men. They were accompanied by a brass band and additionally, a trio of folk musicians. The procession moved through the church and wound its way through village streets and a long curvy hill before retracing its steps back to the church. Along the way, fireworks were set off with loud explosions and smoke.
A man carrying fireworks that he just purchased.
On way downhill to the church (my friend, Cristina in the blue blouse).
In the evening, the plaza was filled with people listening to musical bands. LOUD bands playing a variety of music from marimba to rumba to salsa and more! I could feel the pulsation of the music in my heart while I tried to sleep that night. Thank goodness my party-heavy Brooklyn neighborhood prepared me for this. [It all makes sense now.] The next day, Monday, was a school holiday so there was no holding back on the festivities.
For some reason, I cannot upload my videos so I will look for a different wordpress theme that is more video friendly. Expect changes, in life and on this site.


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Note: This update is not available by default for all Dash users. If you would like to try it out, you can access it by clicking the "Try our New Team Page" in the upper right hand corner.
The Class, Camp, and Team pages have a brand new design and user experience.
How it Looks
There is a left navigational bar to help access all of the different components that make up the specific program you are viewing.
This section includes all of the primary set up information for the group.
On the roster tab you will find summary information for the next event, group financials, group notes and assigned employees. In the roster toolbar are buttons for adding players or managers, and a whole suite of reports and group actions: mass invoice, group invite, group message, audit log and email history can all be found here.
For customers that did not fully pay for their invoice for a roster, it will display on the Roster page, their balance owed.
When adding participants from the Roster page, press the "Add" button. Managers added to teams will display a "Star" next to their name. A pop up modal will appear to search the customer database. In this box, you can enter names, emails, phone numbers, customer ID, etc. Also once you put your cursor in the box, you will notice that your recent search and any newly created accounts will appear for you to select from. If the roster has a custom form attached, this will appear in this box to fill out as well.
From this tab you can add events and see the group schedule. In the toolbar you'll also find links to the League Schedule and the League Analyzer. Should you have an upcoming event, this will be called out at the top of this page and the roster page. The full event list is color-coded so you can distinguish between past and future events.
Here you will find the list of group payments. Should this group have the normal team billing type, you will also see the team invoice here. At the bottom of the group payments list, you will find the link to the invoice summary page should you need it.
This tab will only appear for the "Team" type.
Purchase Orders
Should you have any purchase orders assigned to the group, you will be able view these to edit and/or fullfill them.


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S-5-S Clamps
The S-5-S clamp was created specifically for popular snap-together profiles—including residential profiles by Taylor Metals and Easy Lock Standing Seam. For horizontal seams under .540 inches (like the Firestone UC4) the S-5-S or S-5-S Mini clamp can be used to avoid the necessity of crimping the seam.
Example Profiles
S-5-S and Mini Seam Profiles


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Google is increasing its security and systematically blocking sites that are not securely encrypted with an SSL (Secure Socket Layer) certification. SSL provides a layer of protection that encrypts communications over a computer network, and ultimately increases security. Oh yes! Or at least starting for … Read More
Drafting an Analytics Measurement Plan for Your Website
When you first set out to build your website, you may spend a lot of time considering the fonts, photos, written content and resources the public will interact with on your website, but neglect the time to think about the data your website should track. If … Read More


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Copyright Mountain Meadow Farm. All Rights Reserved.
Betty Lu
Mountain Meadow Farm Betty Lu has a rich chocolate out-of-fleece color and a caramel type B in-fleece color. Her processed cloud is a soft taupe color.
Her fleece is thick, soft and abundant. Like her mother Cornell, Betty Lu's fleece can be plucked or sheared.


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After another three-win weekend, a 6-game winning streak and an 8-game unbeaten streak, the Indy Fuel are in a great position, with three more home games this week, all against East Division foes. It's a great opportunity to keep rolling and build momentum going into a February chock-full of division games.
Current record: 13-21-7, 6th North Division
Make sure to check out Tony Brown's weekly team preview
Last week: The Fuel had a 3-0-0 weekend and continued a six-game winning streak. They pasted the team with the league's best record, the Allen Americans, 4-1 on Friday. Then, they knocked off Evansville by a 4-1 score on Saturday in Indy before an OT win in Evansville by a 2-1 count on Sunday. … On the weekend, Kyle Stroh had the team's first-ever hat trick on Saturday, and scored his 6th game-winning goal on Sunday, which was also the first-ever OT winner for the Fuel. He had a 4-1-5 line in the three games. Garett Bembridge also tallied points in the three games and was 2-2-4. Mike Duco had a goal and two assists, as did Rhett Bly. Dillon Fournier and Justin Holl had two assists each. In scoring 10 goals, 14 different Fuel skaters (and one goalie) got on the scoresheet. … Mac Carruth had the first goalie point of the year with an assist on Mike Duco's empty-netter on Saturday night. Carruth nearly scored a goal, too. He was named the ECHL's Goaltender of the Week on Tuesday. … The line of Stroh-Bembridge-Vincent Arseneau had seven of the Fuel's 10 goals on the weekend. … Mac Carruth won back-to-back games and Shane Owen won Sunday's game, with each allowing just one GA in each game.
Fuel bits: Several streaks are active right now for the team – six straight wins, eight games without a regulation loss (7-0-1), eight games allowing two goals or less in regulation, five games allowing one goal or less total … The winning streak and road winning streak (4 games) are both the second-longest active ones in the ECHL to Colorado (10/5, respectively) … Mac Carruth has won five straight starts and has a 0.98 GAA and a .965 save percentage in those games. Shane Owen is 2-0-1 in his last three starts with a 1.30 GAA and .956 save percentage … Stroh now has six goals and six assists in the last seven games, and is +9 in those contests. He has a seven-game scoring streak going. That point streak is the fifth-longest active streak in the ECHL … Stroh has 12 points in 10 games since joining the Fuel (6-6-12) after tallying 14 points in 32 games with Rapid City … Bembridge has a four-game scoring streak going and has taken over the team lead in goals (15) and points (30). With the Fuel, he has a 13-11-24 line in 31 games. … Mike Duco also has a three-game scoring streak going, and with his 10th goal, became the third player currently on the roster to score 10 with the Fuel this season … Dillon Fournier's two-assist game Sunday was his first multi-point game of the season … Stroh has six game-winners, making him second in the league. He had the GWG in each of the last two games … Mac Carruth's recent hot streak has moved him to 17th in the ECHL in GAA (2.69) and 16th in save percentage (.905). … Matt White's 2 SHG place him second among ECHL rookies … Nick Bruneteau (3-19-22) is tied for 16th among ECHL defensemen in points and ninth in assists … The Fuel remain the fifth-least penalized team in the league (14.33 PIM/game) … In a Fuel uniform, Indy has four active 20-point players: Bembridge (13-11-24), Bruneteau (3-19-22), Rhett Bly (8-13-21) and Duco (10-10-20). Justin Holl (3-16-19) is close … Matt White is the only player to suit up for the Fuel in all 41 games this season … The Fuel PK is 31 for its last 33. … Indy's PP is now sixth in the ECHL (19.8), and the PK is up to 17th (82.2).
This week
Wednesday: vs. Gwinnett Gladiators (14-22-2), 7:05 p.m., Indiana Farmers Coliseum. TV: HTSN-6.2, Radio: WXNT-1430
Season series: Fuel lead 1-0-0
Season meetings: 1/19 at GWT: Fuel 1-0This marks the teams' second meeting in a nine-day span, and the final meeting between the two teams this season. They had a doozy of a game last Monday in Gwinnett, with the Fuel winning 1-0 on a third-period goal by Kyle Stroh, assisted by Garett Bembridge and Nick Bruneteau. Mac Carruth made 26 saves for his first Fuel shutout, and Kent Patterson stopped 21 of 22 Fuel shots in the Gwinnett net. … Since, the Gladiators have gone 2-1, beating a powerful Florida team twice (7-5 and 4-3 in OT) and then falling to Reading 6-3. Greg Carey had three goals and three assists, including a hat trick in Friday's 7-5 win over Florida. Casey Pirro-Zabotel had five goals and three assists, including a three-assist game Friday, a hat trick in Saturday's 4-3 OTW, and two goals in Sunday's loss to Reading. Patterson and Adam Brown split the net duties in the three games. … Gwinnett was just 6-11 on the PK in those three games, including 2-6 against Reading, and 1-9 on the PP. … Pirro-Zabotel (18-27-45) is third in the ECHL in scoring and tied for third with seven PPGs. He is the only player with double-digit goals. Garry Nunn (7-24-31) and Brenden Walker (9-16-25) are the second and third-leading scorers. … Patterson is 2-1-0 with a 3.54 GAA with Gwinnett, and Brown is 5-7-0/3.34. … Gwinnett is 4-11-0 away from home … Gwinnett has the league's seventh-best PP (19.5) and ranks 28th on the PK (77.4). … A few old friends are on the Gladiators' roster – former Ice Will MacDonald and former Fuel Travis Ouellette. A third, former Ice D Tristin Llewellyn, was traded to Wheeling over the weekend. … Gwinnett is the ECHL affiliate of the Arizona Coyotes.
Friday: vs. Reading Royals (25-13-2), 7:35 p.m., Indiana Farmers Coliseum. TV: HTSN-6.2, Radio: WXNT-1430
Season series: Reading leads 4-0-0
Previous meetings: 11/22 at REA: REA 4-3; 11/23 at REA: REA 2-1; 12/17 at Indy: REA 6-4; 1/3 at Indy: REA 3-1
This is the fifth of eight meetings between the two teams this season. Reading has won the previous four, but all were close games, and all came before the Fuel's significant roster overhaul that came in the first full week of January. … The teams meet again March 27, and the Fuel's next trip to Reading will close out the season April 10-11. Reading is the fourth-most-frequently faced foe for the Fuel this year, and the most-frequent opponent outside the North Division. … Reading has won four straight games, including two last weekend against South Carolina (2-1) and Gwinnett (6-3). Maxim Lamarche had a goal and two assists and Adam Hughesman two goals to lead the Royals last weekend. Connor Knapp stopped 38 of 39 to beat South Carolina, and Ken Reiter stopped 30 of 33 to beat Gwinnett. … Pat Mullane (14-25-39) is the Royals' top scorer and ranks 18th in the league in points. His five shorthanded points lead the league … Ryan Cruthers (9-21-30) has 12 PP assists, second in the ECHL … Mike Marcou (4-21-25) ranks 10th among ECHL defensemen in scoring and fifth in assists. He has 10 assists on the PP (2nd in the ECHL) and 12 points with the man advantage (3rd in the league) … Besides Mullane, Olivier Labelle (15-18-33), Adam Hughesman (14-19-33) and Cam Reid (12-20-32) are the Royals' top scorers. … Knapp is the leading active goalie, at 12-7-0/3.02 … Reading is the ECHL's second-least penalized team, taking just 10.88 PIM/game. The Royals play at Evansville on Wednesday before coming to Indy. … Reading ranks 12th in the ECHL on the PP (18.4) and 13th on the PK (82.6). … Reading's +12 goal differential in the third period is 4th in the ECHL … Reading ranks 6th in the ECHL in scoring (3.48 goals/game) and 14th in GA (3.05) … Reading launches 33.8 shots/game, 2nd in the league. … Reading is in the first year of an affiliation with the nearby Philadelphia Flyers.
Saturday: vs. Elmira Jackals (21-16-5), 7:35 p.m., Indiana Farmers Coliseum. TV: HTSN-6.2; Radio: WXNT-1430
Season series: First meeting
This is the first of four meetings between the Fuel and Jackals – they were initially scheduled to play last Friday night as part of a three-game road swing into the Midwest, but Elmira and Allen flipped opponents, and the Jackals faced Brampton for three straight contests. Elmira won two of the three – 3-1 on Friday and 4-2 on Saturday, before falling 4-1 on Sunday. The weekend before, the Jackals went 1-1-1 against Reading. … Forward Allen McPherson led Jackals scorers with five assists in the three games last weekend. Defensemen Steven Shamanski and Zach Tolkinen each had two goals and an assist. Goaltenders Sam Marotta and Travis Fullerton split net duties, with Marotta stopping 38 of 43 shots in two games and Fullerton getting 33 of 35 in a win. Elmira's PP was 4-11 over the weekend and the PK allowed four goals on 13 chances. … Shamanski leads Elmira in scoring from the blueline (11-17-28), while forward Matt Tassone has 24 points (14-10) in just 24 games. They are the lone double-figure goal scorers for the Jackals. Shamanski ranks 10th among rookies in points, and leads rookies and defensemen with five PPG and 13 power play points. He is also the fourth-highest-scoring defenseman in the ECHL, and ranks second among league D in goals scored … In net, Marotta is 8-11-1/2.56, while Fullerton is 13-5-4/2.58. They rank 10th and 12th in the ECHL in GAA … The Jackals are sixth in the ECHL in PIM (20.24/game). They are 10th on the PP (18.6) and 26th on the PK (79.2), but are third in the league with seven shorthanded goals, and are the league's only team to not allow a shorthanded goal this season … Elmira outscores teams in the first and third, but a minus-16 goal differential in the second is second-worst in the ECHL … Elmira ranks 23rd in the league in goals scored (2.71) but is in the top 10, ranking ninth, in fewest goals allowed (2.83). … The Fuel and Jackals meet again Feb. 20-21 in Elmira … Elmira plays at Cincinnati Wednesday and at Fort Wayne Friday before coming to Indy. … Elmira is in the first year of an affiliation with the Buffalo Sabres.
Roster moves: The Fuel's most significant roster move last week was placing forward Jonathan Parker on the 21-day IR. Jason Torf dressed as an emergency backup on Friday, but Shane Owen was ready to return Saturday night. Several players were moved on and off the reserve list to keep fresh players on the ice given the number of games in a short amount of time the Fuel were playing.
Current unofficial roster
Forwards (10)
7 Dean Chelios (6-2, 185, Bloomfield Hills, Mich.). Age: 25. Shoots: L. Last team: Toledo Walleye (ECHL) (Fuel)
9 Rhett Bly (5-10, 185, Regina, Sask.). Age: 25. Shoots: L. Last team: Merrimack (NCAA) (Fuel)
11 Kyle Stroh (6-1, 185, Vibank, Sask.). Age: 26. Shoots: R. Last team: Rapid City Rush (ECHL) (Fuel)
12 Evan Vossen (6-0, 196, Swift Current, Sask.). Age: 28. Shoots: L. Last team: Missouri Mavericks (ECHL) (Fuel)
*14 (C) Mike Duco (5-10, 200, Toronto, Ont.). Age: 27. Shoots: R. Last team: Orlando Solar Bears (ECHL) (Fuel)
*19 Nicklas Lindberg (5-11, 190, Stockholm, Sweden). Age: 34. Shoots: L. Last team: St. Charles Chill (CHL) (Fuel)
20 Matt White (6-2, 198, McMurray, Pa.). Age: 23. Shoots: L. Last team: Vermont (NCAA) (Fuel)
21 Johnny McGuire (6-1, 205, Peterborough, Ont.). Age: 21. Shoots: R. Last team: Idaho Steelheads (ECHL) (Fuel)
27 Vincent Arseneau (6-2, 200, Iles-de-la-Madeline, Quebec). Age: 22. Shoots: L. Last team: Ontario Reign (ECHL) (Manchester Monarchs)
*41 (A) Garett Bembridge (6-0, 180, Saskatoon, Sask.). Age: 33. Shoots: R. Last team: Missouri Mavericks (ECHL) (Fuel)
Defensemen (8)
2 Anders Franzon (6-3, 196, Plattsburgh, NY). Age: 26. Shoots: R. Last team: Missouri Mavericks (CHL) (Fuel)
4 Nick Bruneteau (5-10, 182, Omaha, Neb.). Age: 22. Shoots: R. Last team: Reading Royals (ECHL) (Fuel)
5 Nick Jones (6-2, 200, Cranberry, Pa.). Age: 24. Shoots: L. Last team: Worcester Sharks (AHL) (Worcester Sharks)
10 Dan Ford (6-1, 195, Skaneateles, NY). Age: 24. Shoots: L. Last team: Reading Royals (ECHL) (Fuel)
15 Sam Posa (5-10, 176, Grand Blanc, Mich.). Age: 22. Shoots: L. Last team: University of Windsor (OUAA) (Fuel)
23 Dillon Fournier (6-2, 187, Montreal, PQ). Age: 20. Shoots: L. Last team: Rouyn-Noranda Huskies (QMJHL) (Blackhawks)
24 Kirill Gotovets (5-11, 194, Minsk, Belarus). Age: 23. Shoots: L. Last team: Milwaukee Admirals (AHL) (Rockford IceHogs)
25 Justin Holl (6-3, 195, Tonka Bay, Minn.). Age: 22. Shoots: R. Last team: Minnesota (NCAA) (Rockford IceHogs)
Goaltenders (2)
1 Shane Owen (6-2, 195, Markham, Ont.). Age: 24. Catches: L. Last team: Stockton Thunder (ECHL) (Fuel)
35 Mac Carruth (6-2, 160, Jackson, Wyo.). Age: 22. Catches: L. Last team: Rockford IceHogs (AHL) (Blackhawks)
Reserve (2)
16 Pete Massar (5-9, 180, Williston, Vt.). Age: 25. Shoots: R. Last team: Missouri Mavericks (CHL) (Fuel) F
*88 (A) Garrett Klotz (6-6, 250, Regina, Sask.). Age: 25. Shoots: L. Last team: Allen Americans (CHL) (Fuel) F
21-day IR (1)
22 Jonathan Parker (5-10, 195, Solana Beach, Calif.). Age: 23. Shoots: R. Last team: Colorado Eagles (ECHL) (Fuel)


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ᓱᒃᑲᔪᒥᒃ ᖃᕋᓴᐅᔭᒃᑯᕈᓐᓇᐅᑎᖃᕐᐲᑦ? ᓱᒃᑲᔪᒧᐊᕐᓗᑎᑦ
ᑕᕆᔭᕋᓱᒃᑕᐃᑦ ᓱᒃᑲᐃᓗᐊᕐᐸ? ᐊᓯᓪᓕᕐᓗᒍ ᓱᒃᑲᐃᓂᕐᓴᒧᑦ ᑕᑯᒃᓴᐅᓇᕐᑐᒧᑦ
Uploaded by: IBC admin
17 March 2019
896 views
Program: Qimavvik – was an Igloolik cultural show including storytelling, hunting and sewing techniques, legends, language, igloo building, etc.
Producer: Igloolik Inuit Broadcasting, Nunavut
Host: Julie Ivalu
Segment 1: Baker Lake, Nunavut, elder Ada Iyi'uaq Kingilik, recalls a story during times of local starvation.
Segment 2: Igloolik residents convey their Christmas greetings to relatives and friends.
The Inuit Broadcasting Corporation, established in Canada's territory of Nunavut, has been producing Inuit language video, by, for and about Inuit since 1981. This video is from their collection and has been made available on the Internet for your enjoyment through funding provided by the Government of Canada and the Government of Nunavut.


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Is it a bird? A plane? Superman? No, it's a giant curse word floating in the sky.
The elusive F*ck Cloud was spotted this week by a Redditor and, despite a few skeptics saying that the swear word cloud is fake, it appears that this is a genuine, authentic, real cloud cursing at the world.
Brennan61 shared the photo this week on Reddit. The image quickly made its way to the front page where it received more than 2500 upvotes and another 550 comments.
The Redditor writes:
"I can't believe it. I turned around and saw the word "fuck" written flawlessly in the sky. This was my special moment. It may be my MOST special moment ever. Thank you for understanding. I have two eye witnesses to my absolute "fuck cloud" freakout I had when I noticed it."
In addition to the awesome photo, Brennan61 also posted a video of the swear word cloud. The Redditor said that he posted the video to give definitive proof that the F*ck cloud does indeed exist.
The Redditor writes: "That's why I posted the video too. I want people to know that mother nature actually did that for me, and for us."
Here's a video of the curse word cloud.