text:
The Northern fur seals that breed on the Pribilof Islands have been on the decline for decades, a smaller colony just 200 miles away is thriving. A new study of these colonies is challenging scientists' assumptions about what marine animals need from their environment — and how they get it.
Listen to the full story


text:
There was a topic opened on the GP forum:
http://gp.worldpuzzle.org/content/wpf-a ... -interests
WPF director said in April 2017:
"We will attempt to iron out a plan for future competitions, but that is not happening any time soon nor is it immediately necessary, so I request that we table the discussion for now. At the time when the WPF is ready to divulge a full plan, we'll be open to discussion and thoughts from everyone."
It seems that WPF director and some board members will take part officially in the next WSC/WPC. But what said WPF director wasn't done. Was there a vote of WPF members about this subject?
Can you please inform us about this subject.
Thanks !

subset: commoncrawl


text:
Item was successfully added to your cart
View Cart
A millefeuille of rock lightened with silt and clay, plus a sprinkling of stones and pebbles. The small feeder roots of the vines work themselves completely into the horizontal layers of this very unusual for Oregon rock pastry. These basalt rocks are all about the fractures providing space for tiny amounts of clay (a unique microbiome) and feathery roots, which provoke great tension holding together its mineral and fruit driven sides. Like a suspension bridge anchored by opposing notions of flesh and bone with high toned red fruit connected to mid-tone blue and black flavors creating a liquid harmonic wave. Plush richness with ripe raspberry and pomegranate flavors accented by plum, tea, mineral and spice notes. Explore the soil layers →


text:
Marty St. Louis was still in a walking boot on Thursday, but that is not stopping the Lightning captain from perhaps playing tonight against the Wild at the Tampa Bay Times Forum.
"Marty is a game-time decision," coach Jon Cooper said. "We'll see what happens in the next few hours. He's an option to play."
St. Louis, who is off to a terrific start with 10 points on four goals and six assists in six games, was hit on his right foot by a Drew Doughty slap shot early in the third period of Tuesday's 5-1 victory over the Kings.
Cooper said all tests were negative in that there are no broken bones and St. Louis cannot do more damage to the spot where he is hurt. The issue, then, is simply pain tolerance.
"I just don't want someone in there who is going to be apprehensive, not being the Marty we're used to seeing," Cooper said. "We'll find out game time. That will determine Brett's fate."
Cooper was talking about Brett Connolly, who was called up Wednesday night from AHL Syracuse, and who skated in the morning in St. Louis' right wing spot on a line with center Steven Stamkos and Ryan Malone.
Whether or not to play St. Louis is an interesting decision. If he is willing and cannot further his injury, "Why wouldn't we play him?" Cooper said.
The schedule probably is a consideration in that Tampa Bay after games tonight against the Wild and Saturday against the Bruins, have four days off before it plays Oct. 24 against the Blackhawks. That would give St. Louis some healing time.
There also is the Vinny Lecavalier factor. Lecavalier was off to a great start last season when he was hit in the foot by a Sami Salo slap shot at the end of a Feb. 2 game against the Rangers. Lecavalier tried to play through the injury which he admitted later hampered his game.
"Ultimately, in the end, the decision will be up to the staff to determine whether he's going to play or not," Cooper said. "Marty has played in enough games to know if he can or if he can't go. Sometimes you have to take the players' competitiveness out of it, knowing how much he wants to play. We will talk to him about it, but I highly doubt he's going to walk out saying he doesn't want to play."
Other stuff from the morning skate: As expected, Ben Bishop, who is 4-0-0 with a 1.71 goals-against average and .939 save percentage, will get the start against the Wild. ... Defensemen Mark Barberio and Keith Aulie will be scratched for the sixth time in seven games. ... Cooper said St. louis' injury will not deter him from using his star players on the penalty kill because blocking shots is part of the game. "It used to be something that just the warriors blocked shots. Now, it's just about everybody blocks shots." Cooper pointed out that Malone scored his first goal of the season against the Kings when Salo's shot deflected in off his foot. "What if (he had gotten hurt)? Cooper said. "Would people be saying you shouldn't be in front of the net on a five-on-five? The way the game is played, the way guys shoot, the only way to really protect yourself is to wear protective padding." Bottom line, Cooper said, "The second we're fearing a guy getting hurt on a blocked shot, we're probably in the wrong sport." ... Added Stamkos, who also is being used more on the penalty kill: "Five-on-five you're going to block a shot, penalty kill you're going to block a shot, power play. If you have to block a shot you'll block a shot, so I don't think that changes anyone's mind-set or anything like that." ... Wild assistant coach Darryl Sydor, a member of the Lightning's 2004 Stanley Cup team, got his first look at the renovations to the Times Forum, especially the oversized scoreboard. "I'm not sure if I'm going to watch the screen tonight or the ice?" he said. ... Zenon Konopka was known best for his fighting when he was with the Lightning from 2008-10, but he also was great at faceoffs. He still is and has won 40 of 56 draws this season. "It's a game within a game," he said. "You're trying to figure out what the other guy is going to do. if you can do that, you can counter." Konopka says he keeps a book "in your head" about opponent tendencies. "How their feet will set up, how high or low the guy's hands are on the stick," he said. "For some reason for me it clicks probably a lot quicker than other guys." ... Loved this quote from Wild coach Mike Yeo about how players should manage games in which they are not at their best. "You can have an off night, just don't make it a bad game." ... Connolly, wearing an "A" as an alternate captain, has just an assist in three games for Syracuse, but said a lot of his time has been about bringing together a team with personnel different from last season. "Just trying to be a leader and do the same things I did last year, not only play well but be a good teammate," he said. As for the disappointment of not winning a job with the Lightning out of camp, he said, "I just wanted to go down there (to Syracuse) and have a good attitude. I didn't want to go down there and sulk and be mad about it. I'm happy to be up here now and I'll be ready if they need me."


text:
| 9.3°C Belfast
Arsene Wenger has backed reserve keeper Lukasz Fabianski and the rest of his young Arsenal team to rise to the challenge when they take on Partizan Belgrade in what is set to be a highly-charged Champions League encounter in Serbia on Tuesday night.
The Gunners were shell-shocked by their home Barclays Premier League defeat to West Brom on Saturday, where they had trailed 3-0, and now must look to get their season back on track with a positive result in one of the most intimidating atmospheres in European football.
"The disappointment was very deep, but we can learn from what has happened to us," said Wenger.
"We realise that we need to be focused in every single game, produce performances in every single game and that is what I believe we have learnt from Saturday.
"I believe in the intelligence of my group players and I have no doubt in their talents.
"We will be highly motivated and will try to redress what was not right on Saturday."
Manuel Almunia - who made a number of errors - picked up an elbow injury in that game which opens the door for Fabianski tomorrow, with the Pole looking to take his chance with both hands rather than drop another clanger.
Wenger continued: "For Fabianski, it is another big chance - but you want a goalkeeper or any player to grab the chance when you get it.
"As a manager you can give a chance to a player who is good enough and I believe in his talent. He gets another opportunity to show how good he is. I want him to show how good he is.
"There is only one way to show confidence in a player and that is to put him on the pitch and give him his chance."


text:
I love experimenting with food. There is a part of me that is very traditional about certain aspects of cooking and remaining true to the traditional Persian and Italian recipes. Yet, there is the rebellious side of me who likes to experiment. I am slightly schizophrenic when it comes to this though because you never know which recipes I am not willing to deviate from in any shape, way, or form. One thing is sure though, it is mostly the traditional Persian recipes that I can't deal with changing.
I had a rebellious thought a couple of weeks ago and decided to act on it. I was craving rice and didn't have anything around for a traditional dish. But truth be told I needed to be creative in order to refocus from my writing projects. I had some mushrooms in the fridge so I decided to seriously experiment. What I mean by seriously is using my rice cooker in ways that I had not before. I thought the results were good and most definitely worthy sharing.
If you are Persian, you will likely be horrified because that's not how we are taught to use our rice cookers. But please trust me, it worked.
I welcome, and encourage, you to leave a comment if you choose to make this recipe or have done something similar. I would love to know how others experiment.
4 cups of rice
12 oz package of crimini mushrooms sliced
1 onion diced
2 cloves of garlic minced
1 tbls of advieh
canola oil
salt & pepper
A Persian rice cooker (this will not work in a regular rice cooker)
Sauté onion and garlic until onion is golden. Add 1/2 tbls of advieh.
Wash rice and place in the rice cooker with 4 cups of water. Add a generous amount of oil (1 1/2 tbls) and salt (2 tsp) and give it a little stir. Add the sliced mushrooms on top. Turn on rice cooker. Trust me….
In about 15-20 minutes or so the water will start absorbing. When you noticed that most of the water has been absorbed, and all you can see is bubbles, take out the mushrooms and place them in a bowl. Then scoop out the rice as well only leaving a thin layer at the bottom of the rice cooker. I know…you don't take the rice out of the rice cooker, but hang on to your panties, it works, I promise!
In the bowl mix rice, mushrooms, onion mix, and the remaining advieh. Taste for seasoning and if needed add more salt, pepper, or advieh.
Place rice mixture back in the rice cooker. Place a kitchen towel around the lid and cover. You say: "but you are not supposed to cover the rice cooker's lid with a towel." I say: "I have experimented enough to know that it works better this way!"
Now, all this new found method is likely to mess up the timing on the rice cooker. So cook for about 45-50 minutes and then turn off the rice cooker.
Flip the rice onto a dish and the results should, hopefully, look just like the picture above!


text:
Toxic Games have been showing off their upcoming sequel Q.U.B.E. 2 at different trade shows for a few months. Giving any lucky soul not only the chance to see it in action but to get their hands on an early demo. Couldn't make it to PAX West or EGX, me either. Don't worry though; Toxic Games were kind enough to throw together an eight-minute video for us ordinary folk who couldn't make it.
When compared to the original, two differences blatantly stand out. The first is the massive jump in graphics, the original is slightly dull and cartoonish, whereas now it looks somewhat shiny and more realistic. The other noticeable difference between the two is how the puzzle system works. In the original, colored blocks were scattered around the environment, the color telling you what that specific block did when activated. Now instead of the blocks being pre-colored, they are marked as usable, and you choose the color yourself.
Q.U.B.E. was initially released for PC in 2011, not coming to consoles until 2014, which is when they added the "story." This time around, Toxic Games is focusing on the story and making sure it is an integral part of the game.


text: Complete control for MMO victory Programmable 12-button design optimized for MMO gaming Increased flexibility – play in wired or wireless modes Easy-swap switchable socket design for customizable click resistance Customizable ASUSAura RGB LED lighting effects across three zones, with Aura Sync support inwired mode Solidly-built magnesium alloy chassis Built-in flash memory to save your favorite profiles Exclusive ROG Armoury interface for easy customization of buttons, performance, and light settings. 8200 DPI, 150 ips, 30g acceleration and 2000Hz USB polling rate supported in wired mode for pixel-precise mouse tracking 3D-printing-friendly design Shop Modal header WATCHTHE VIDEO Omron® switches with 20-million-click durability Easy-swap switchable socket design Six tactile thumb buttons Mayan-patterned rubber grip Magnesium alloy chassis Magnetic charging station Alps® encoder scroll wheel 2-level DPI switch LED battery indicator 20 million-click durability Omron switchesEasy-swap switchable socket design Six tactile thumb buttons Mayan-patterned rubber grip Magnesium Alloy Chassis Magnetic charging station Alps encoder scroll wheel 2-level DPI switch LED battery indicator 0% Twelve programmable buttons for MMO gaming ROG Spatha has a total of 12 programmable buttons, including six thumb buttons, to give you intuitive command and control. It fits perfectly in your hand, and has been ergonomically-designed to give you tactile feedback and response. Customizable switch socket design Gaming mouse buttons take a lot of punishment, and that's why the buttons on ROG Spatha feature durable Omron® switches with a 20-million-click lifespan. The exclusive switchable socket design allows for easy customization too. ▶Learn more about the ASUS-exclusive switchable socket design 20-MILLION-CLICKS Six programmable thumb buttons ROG Spatha comes with fully-programmable thumb buttons with Alps® switches to give you tactile feedback. The six buttons are arranged to resemble the iconic ROG eye and are designed to provide intuitive control and feel. Specially-designed micro-USB cable prevents the cable dragging on desk. The left and right buttons are independent of the mouse body for a tactile click response. The DPI clutch enables instant switching between two sensitivity levels: - Standard speed for everyday gaming - Extra-low speed for better aiming and accurate sniping The scroll wheel features an Alps encoder for a positive stepped action. Premium magnesium alloy chassis for increased durability. Special steel-gray non-stick coat and textured Mayan-stenciled rubber panels for an assured grip. ROG Spatha's 8200DPI laser sensor and 2000Hz polling rate give you extreme precision and response – even the slightest movement will be tracked. Ergonomic right-handed design that's ideal for palm- and claw-grippers. wireless and Wired – it's your choice ROG Spatha utilizes a high-speed wireless connection for high data throughput and low latency to ensure all your movements are tracked accurately. And once the battery runs out, you can continue using it while charging, all without losing response and performance. WIREDMODE WIRELESSMODE Low battery level Medium battery level Ready Customizable light effects across three zones ROG Spatha has an aggressive angular design that stands out. You can even customize the LED light effects on the scroll wheel, side buttons and ROG logo from a wide range of colors. Experience ROG Spatha's lighting modes outshine the competition Perfect Lighting In Sync ASUS Aura Sync technology takes RGB lighting beyond the checkbox by letting you control the LEDs on all of your Aura-enabled products. Achieve perfect, harmonious lighting effects across your motherboard, ROG Spatha, and an ever-growing portfolio of Aura-enabled products — all from a single application. Learn more about Aura Sync > Note 1: ROG Spatha supports Aura Sync in wired mode only. Synchronization between the ASUS motherboard, graphics card and ROG Spatha is enabled and controlled by Aura-ready motherboards. Visit the support page for your Aura-enabled motherboard and download Aura software by clicking Driver & Tools > Utilities. Note 2: To synchronize Aura between ROG Spatha and Claymore only, open the ROG Armoury and select the Claymore lighting tab. While the Armoury is activated, press and hold the Fn key and then press the ROG SYNC hotkey. Synchronization will take about 10 seconds. To stop synchronization, repeat the same process. Prepare for battle with ROG Armoury Customize user profiles, lighting effects, and program macros via the intuitive ROG Armoury dashboard. ▶Built-in flash memory to save five profiles EXCLUSIVE EASY-SWAP SWITCHABLE SOCKET ROG Spatha features an exclusive easy-swap switchable socket design for easy upgrades. All you have to do is unscrew the four bottom screws with the bundled screwdriver to release the top cover and gain access to the sockets. *Bundled screwdriver required 3d printing friendly The new era of customization. Make it your own. 3D printing goes beyond mere aesthetics: It allows you to design and create a mouse that truly fits your hand and grip, with a look that is distinctly yours. Ring finger rest (Original design) No finger rest Ring and pinkyfinger rest Customize and 3D print in four simple steps Download Select the 3D source files you want to download. Personalize Adjust size or shape, add text or change other attributes. Print Use your own 3D printer, or find a local vendor or an online vendor. Attach Easily fit your new 3D-printed part into existing mounting holes. BUILT FOR GAMERS Gaming is in our blood, and our R&D engineers worked extensively with professional gamers, took their feedback into consideration, and developed a gaming mouse that's designed to withstand the rigors of marathon gaming sessions. ROG Spatha is engineered for performance and durability, and stands ready for you to take into battle. Game on, anywhere! ROG Spatha features its own hard case and gives you the convenience of one braided and one rubber USB cable. Simply connect one to the charging station, and keep the other one together with Spatha in the stylish hard case to take to LAN parties. Package contents: 1 x wireless receiver/ charging dock 1 x 2-meter braided USB cable 1 x 1-meter rubber USB cable 2 x Japanese-made Omron switches 1 x carry case 1 x star-head screwdriver 2 x ROG logo stickers 1 x ROG certificate Superior Specs Intuitive control Ultimate customization Built for gamers Best mobility


text:
Senior Contentious Trusts & Probate Solicitor
- Employer
- JMC Legal recruitment
- Location
- England, Surrey, Reigate
- Salary
- £45000 - £65000 per annum + Excellent Benefits
- Closing date
- 5 Oct 2020
View more
- Practice area
- Private client
- 5 years, 6 years, 7 years+
Senior Contentious Trusts & Probate position | Top 200 firm
Are you a Solicitor looking to get a broader range of experience in Contentious Trusts & Probate? The opportunity has arisen to join this Chambers rated and Legal 500 practice, which deals with City standard work. The firm is one of the largest and well-established practises nationally and deals with a client base that most London firms would be envious of.
The Firm
- This practice is ranked in the top 100 nationally and are regarded as market leaders
- They are ranked across multiple areas in the legal 500
- They have a number of Lawyers who are ranked as leaders in the field for both the Legal 500 and Chambers.
- Renowned for excellent client testimonials and establishing deep relationships, this firm stands out throughout the South-East
- The firm creates an enjoyable atmosphere for their staff and has that translate into positive experiences for its clients. This culture is epitomised by the partners and cascades through every team member and department.
- They deal with HNW work with some covering an international scale
- For go getters the sky is the limit; the firm will provide distinctive training and give its staff the opportunity to grow within the practice organically.
Key Points - Contentious Trust Lawyer (5+ year PQE)
- The firm is looking for someone to join their growing team based in Reigate.
- Role responsibilities would include but not be limited to; advising trust companies, acting on trustee disputes, estate disputes where there is the issue of capacity, court of protection, and asset freezing.
- It is important to highlight that although it would be a benefit to have the above experience, they could consider training someone up in this area.
- The firm handles very high-profile work
- Due to the IT Infrastructure which has now been implemented firm-wide, there is a cohesive and collegiate culture within the practice.
This is a fantastic opportunity for a Contentious Trusts & Probate Solicitor to join a growing practice based in Reigate.
Please feel free to contact me for a private consultation or more information.
JMC Legal Recruitment are a National legal recruitment agency with offices in London, Bristol and New York. Working with Top law firms, a refreshing approach.
Get job alerts
Create a job alert and receive personalised job recommendations straight to your inbox.Create alert


text:
There are no reviews yet.
Your email address will not be published. Required fields are marked *
Your review *
Name *
Email *
This 59" double-interlocking stainless steel hose conveniently works with any PULSE ShowerSpas shower system or any 1/2" standard connection. Finish available: Brushed-Nickel, Chrome, or Oil-Rubbed Bronze.
Actuator for Kauai II and Lanikai


text:
The Bank's in Assassin's Creed Revelations will not automatically give you the money to spend. You will get a notification on your screen every 20 minutes advising you how much has been put in the bank's vault. To get access to this money you must travel to one of the Bank's on your map and choose the option to withdraw the money.
If you find the money is not accumulating as much as you thought, its possible the 20 minute timer has been intterupted. If any of the below occur during this time the timer will reset:
- Linear Game Sequences e.g. campaign missions.
- The Cappadocia section of the game.
- Den Defence.
- Altair's missions.
- Any mission is aborted.
Once any of the above take place in the game, the timer will reset and the full 20 minutes will need to pass again before a deposit is made.


text:
Fragile X mental retardation protein (FMRP) is required for normal cognition. FMRP has two autosomal paralogs, which although similar to FMRP, cannot compensate for the loss of FMRP expression in brain. The arginine- and glycine-rich region of FMRP (the RGG box) is unique; it is the high-affinity RNA-binding motif in FMRP and is encoded by exon 15. Alternative splicing occurs in the 5′ end of exon 15, which is predicted to affect the structure of the distally encoded RGG box. Here, we provide evidence that isoform 3, which removes 25 amino acids from the 5′ end of exon 15, has an altered conformation that reduces binding of a specific antibody and renders the RGG box unable to efficiently associate with polyribosomes. Isoform 3 is also compromised in its ability to form granules and to associate with a key messenger ribonucleoprotein Yb1 (also known as p50, NSEP1 and YBX1). Significantly, these functions are similarly compromised when the RGG box is absent from FMRP, suggesting an important regulatory role of the N-terminal region encoded by exon 15.
Fragile X syndrome is the most common form of inherited mental retardation and is caused by loss of expression of the fragile X mental retardation protein (FMRP, encoded by FMR1) (Bassell and Warren, 2008). FMRP has two autosomal paralogs, FXR1P and FXR2P (encoded by FXR1 and FXR2, respectively), that cannot compensate for the loss of FMRP function in brain (Coffee et al., 2010). FMRP is an RNA-binding protein with two K homology (KH) domains and a region rich in arginine and glycine residues, the RGG box (Bardoni et al., 2001). The RGG box is a high-affinity RNA-binding motif, binding G-quadruplex RNA with nanomolar affinity (Darnell et al., 2001; Schaeffer et al., 2001). Although both FXR1P and FXR2P contain an RGG box and an arginine- and glycine-rich-region, respectively, FMRP is unique in its ability to recognize G-quadruplexes, suggesting that the RGG box of FMRP plays a non-redundant role in the pathology of the disease (Darnell et al., 2009). Because of the key role of the RGG box in FMRP function, it is crucial to understand how this domain is regulated.
The FMR1 pre-mRNA is alternatively spliced at the 3′ end to give multiple FMRP isoforms (Ashley et al., 1993; Eichler et al., 1993). Exon 15, which encodes the highly conserved phosphorylation site (Siomi et al., 2002; Ceman et al., 2003) and the RGG box, has three alternative splice acceptor sites upstream of the RGG box that maintain the RGG box but truncate the N-terminally encoded region of exon 15 (Fig. 1A). All three splice forms have been characterized at the biochemical level (Dolzhanskaya et al., 2008) but not in cells. The RGG box is part of a three-to-four strand antiparallel beta sheet that functions as a platform for nucleic acid interactions in other RNA-binding proteins (Dolzhanskaya et al., 2008). Isoform 3, the isoform with the largest N-terminal deletion in exon 15, is predicted to have a truncated first strand, significantly perturbing the side-chain conformations of the RGG box arginine residues (Dolzhanskaya et al., 2008). To understand the function of the RGG box in cells, we extended the biochemical characterization by Dolzhanskaya and colleagues by examining the cellular function of isoform 3 (Dolzhanskaya et al., 2008). Our data suggest that the N-terminal region of exon 15 is crucial for normal RGG box function, modulating translation regulation and granule formation.
Results and Discussion
The FMR1 mRNA has multiple isoforms in brain, including isoform 3 (Ashley et al., 1993; Xie et al., 2009), but their protein products have not been visualized in the absence of the autosomal paralogs, FXR1P and FXR2P. To examine the brain isoforms of FMRP, we used a serial immunoprecipitation strategy described previously (Ceman et al., 2003). We first isolate the FMRP-containing messenger ribonucleoprotein (mRNP) complex with the FMRP-specific monoclonal antibody 7G1-1 (Brown et al., 2001). The complex is disrupted and eluted by boiling in SDS sample buffer. We then isolate free FMRP with a second immunoprecipitation as described in the Materials and Methods. We found that FMRP exists in at least three isoforms in brain (Fig. 1B, left-hand panel).
The crucially important RGG box is methylated on four of its arginine residues in cells, which accounts for ~90% of the FMRP methylation (Stetler et al., 2006). Isoform 3 is hypomethylated in in vitro assays, suggesting that the region upstream of the RGG box plays a role in regulating FMRP methylation (Dolzhanskaya et al., 2008). In cells, we examined the methylation state of isoform 3 FMRP, wild-type (WT) FMRP and FMRP lacking the RGG box (ΔRGG), which is unmethylatable (Stetler et al., 2006). WT FMRP is encoded by the longest isoform, containing all 17 exons, as described previously (Stetler et al., 2006). In contrast to the in vitro studies, isoform 3 appeared to be hypermethylated compared with the methylation of WT FMRP (Fig. 1C, top panel). To examine the amount of transgene-encoded protein, we probed with the anti-FMRP antibody, KI (Fig. 1C, middle panel). Although this result suggested equal loading, we also probed with an antibody to the N-terminal Flag epitope. Surprisingly, the Flag immunoblot showed that more isoform 3 protein had been loaded (Fig. 1C, bottom panel). These results led to two conclusions: first, that there was no change in the methylation state of isoform 3 in cells (Fig. 1C,D); and second, that the 25 amino acids absent in isoform 3 are crucial for recognition by KI (Fig. 1A).
Comparisons of the ratio of KI reactivity to Flag antibody reactivity demonstrated that these antibodies do not have equal recognition and that isoform 3 has lost epitope(s) recognized by approximately two-thirds of the polyclonal antisera KI but retains an epitope recognized by the remaining antibody. Thus, isoform 3 is recognized approximately one-third as well as WT FMRP or ΔRGG (Fig. 1E). We used this specific recognition by KI to determine whether isoform 3 was present in the brain, as mass spectrometry analysis of brain-derived FMRP did not result in sufficient coverage of exon-15-encoded protein to be informative (personal communication by Benjamin Garcia, Princeton University, Princeton, NJ). Probing of the brain-derived FMRP with an antibody specific to the N-terminus of FMRP (1C3) (Khandjian et al., 1998) detected all FMRP isoforms. By contrast, KI was unable to detect the smallest FMRP isoforms in a comparable exposure (Fig. 1B, right-hand panels), suggesting that the smaller FMRP isoforms are missing the first 25 amino acids of exon 15.
KI was generated using amino acids 516–632 of human FMRP (515–631 in mouse) (Fig. 1A) (Reis et al., 2004) and effectively recognizes ΔRGG (Fig. 1C), which lacks amino acids 526–547. However, KI has a substantially reduced recognition of isoform 3, which is missing amino acids 490–514. Our results suggest that the epitope required for the majority of the KI recognition lies between amino acids 516 and 525, comprising, in part, the antiparallel beta sheet perturbed in isoform 3 (Dolzhanska et al., 2008).
Although isoform 3 has an altered conformation N-terminal to the RGG box, we found that it was normally methylated in both the mouse fibroblast cell line L-M (TK–) (Fig. 1C,D) and the mouse STEK FMR1-knockout fibroblast cell line (data not shown), probably reflecting the ability of protein arginine methyl transferases (PRMTs) to methylate substrate arginine-glycine-bearing peptides without a need for protein context (Gary and Clarke, 1998). Our observation was in contrast to the in vitro studies, which showed that isoform 3 was hypomethylated in reticulocyte extract (Dolzhanskaya et al., 2006). This apparent disparity might be because we examined isoform 3 in cells, which probably express a wider number of PRMTs than do reticulocytes.
The RGG box is required for normal polyribosome association: its removal causes loss of FMRP from the translating polyribosomes (heavier fractions) and a subsequent accumulation of ΔRGG in lighter fractions (Blackwell et al., 2010). To determine whether isoform 3 has compromised RGG box function in cells, we examined polyribosome association in WT FMRP- and isoform 3-expressing cells and found a significant loss of isoform 3 from polyribosomes (Fig. 2, fractions 5 and 6). Accordingly, we found an increase in isoform 3 in fraction 1 compared with WT FMRP (Fig. 2). Our results suggest that the region upstream of the RGG box is important for normal FMRP function, probably through regulation of the RGG box. We suspect that ΔRGG still binds RNAs, given that there are other RNA-binding domains in FMRP (Bassell and Warren, 2008). Isoform 3 also binds RNA in in vitro studies, although with specificities distinct from WT FMRP (Denman and Sung, 2002). Thus, the loss of isoform 3 and ΔRGG from polyribosomes might be due to either less RNA binding by the RGG box or translation suppression of bound RNAs.
It has been reported that expression of ΔRGG in STEK cells results in the formation of fewer FMRP-positive granules compared with upon expression of WT FMRP, although this observation was not quantified (Mazroui et al., 2003). In addition, FMRP isoform 12, which lacks exons 12, 14 and the RGG box, is markedly absent from dendritic granules (Levenga et al., 2009). We hypothesized that the RGG box is required for normal association with granules. Because the presence of a wild-type copy of FMR1 rescues the isoform 12 phenotype, we performed this experiment in STEK cells (Mazroui et al., 2002). To quantify the change in RGG-box-dependent FMRP-containing granules, we used an assay previously described to quantify granules in cultured cells [(Ling et al., 2004); described in the Materials and Methods]. As expected, we found that loss of the RGG box resulted in fewer FMRP-containing granules throughout the cell (Fig. 3A,B, compare WT with ΔRGG). Similarly, expression of isoform 3 also resulted in significantly less FMRP-containing granules compared with WT FMRP. There was no statistically significant difference in granule size, and the granules had an average area of ~77 pixels, equivalent to a diameter of less than 1 μm (Fig. 3C). There was also no statistically significant difference in average granule intensity (data not shown). This decrease in granules containing isoform 3 was not significantly different from the reduction observed with ΔRGG (Fig. 3B). Thus, similar to the polyribosome studies, loss of the region N-terminal to the RGG box has the same effect on function as losing the RGG box.
In addition to the proposed RGG box regulatory domain, isoform 3 also lacks the phosphorylation site at serine 499 (Fig. 1A). To determine whether changes in granule formation were caused by loss of phosphorylation, we measured granule formation of an unphosphorylatable form of FMRP (S499A) (Ceman et al., 2003). Consistent with that previous study, we found no significant difference in granule number compared with WT FMRP (Fig. 3, compare WT with S499A), and there was no significant difference in granule size (Fig. 3C) or intensity (data not shown). To determine whether FMRP loading onto granules requires synthesis of new mRNA, we blocked transcription with actinomycin D and found a significant decrease in FMRP granule formation (Fig. 3, compare WT with WT+act.) but no significant difference in granule size (Fig. 3C) or intensity (data not shown). This result suggests that formation of FMRP-containing granules requires mRNA synthesis and that mRNA is a crucial factor in determining whether FMRP is present on granules.
The results of both the polyribosome and the granule assay suggest that RNA binding by the RGG box is impaired in isoform 3; however, it is also possible that a protein component is affected. In fact, FMRP-containing granules consist of many different proteins (Kanai et al., 2004). The region absent in isoform 3 contains a binding site for microspherule protein 58 (MSP58, also known as microspherule protein 1) (Davidovic et al., 2006) and the C-terminus of FMRP interacts with the Ran-binding protein in the microtubule-organizing center (RanBPM, also known as RANBP9) (Menon et al., 2004). To assess whether any changes in protein association occur upon removal of the N-terminal region of the RGG box or the RGG box itself, we visualized associated proteins by silver staining. We found one distinct band that was reproducibly present for WT FMRP but that was absent for both ΔRGG and isoform 3 (Fig. 4A). This protein migrated at ~50 kDa, similar to a known FMRP-associated protein, Yb1 (also known as p50, NSEP1 and YBX1) (Ceman et al., 2000). Probing an independent immunoprecipitation revealed that the protein absent in both the ΔRGG and isoform 3 samples was Yb1. Like FMRP, Yb1 is an RNA-binding protein found on polyribosomes (Evdokimova et al., 1995; Funakoshi et al., 2003). It is also a core granule protein (Minich et al., 1993; Skabkin et al., 2004) whose association with FMRP is believed to be independent of RNA, suggesting a protein–protein interaction. Yb1 might associate with the C-terminus of FMRP, such that perturbation of the RGG box disrupts association with Yb1, affecting granule formation. Alternatively, Yb1-containing granules might be unable to efficiently recruit isoforms of FMRP in which the RGG box is compromised.
FMRP is a dynamic protein, associating with a large collection of mRNAs that it regulates the expression of [by either facilitating their intracellular localization on transport granules (Antar et al., 2004) or by activating or suppressing their translation (Brown et al., 2001)]. That FMRP has different isoforms might underlie how it has so many intracellular functions and can be found on both translating and non-translating granules (Beaulieu, 2000; Mazroui et al., 2002; Aschrafi et al., 2005). Furthermore, the state of a cell might dictate which FMRP isoforms are present, leading to the execution of a specific function. When P19 cells were induced to differentiate, splice site usage was shifted to 15c, suggesting that isoform 3 is required for neurite production or function (Xie et al., 2009). In conclusion, understanding how the RGG box is regulated will give insight into how FMRP binds its target mRNAs, assembles with granules and regulates translation.
Materials and Methods
Cell lines and assays, DNA constructs and transfection studies
VC (vector control) is the RSV.gpt vector transfected fibroblast L-M (TK–) cell line described by Ceman et al. (Ceman et al., 1999). Cells were grown as described previously (Blackwell et al., 2010). Transient transfections were performed using polyethylenimine (Sigma) at the same concentration as the DNA. Isoform 3 was constructed from WT FMRP using the deletion primers: 5′-CCTGGATATACTTCAGCTCCAACAGAGGAAG-3′ and 5′-CTTCCTCTGTTGGAGCTGAAGTATATCCAGG-3′.
Metabolic labeling was performed as previously described (Stetler et al., 2006) and immunoprecipitations were performed using Flag-M2–Sepharose beads (Sigma). Polyribosome analysis was performed as described previously (Blackwell et al., 2010).
Antibodies and western blotting
The KI antibody was provided by Andre Hoogeveen (Erasmus University, Rotterdam, The Netherlands), the anti-Flag (Sigma) and anti-Yb1 antibodies were obtained from Valentina Evdokimova (University of British Columbia, Canada), the 7G1-1 antibody is as described previously (Brown et al., 2001), the 1C3 antibody was obtained from Jean-Louis Mandel (Institute of Genetics Research, Ilkirch, France). Antibody reactivity was visualized using an anti-mouse-IgG horseradish peroxidase (HRP)-conjugated antibody (KPL, Gaithersburg, MD) or anti-rabbit-IgG HRP-conjugated antibody (Amersham) and developed with ECL (Amersham).
FMRP purification from mouse brain
CBA/CaJ adult male mice were provided by Albert Feng at the University of Illinois at Urbana-Champaign (Institutional Animal Care and Use Committee protocol number 09098). Brains were prepared as described previously (Winograd et al., 2008). To isolate FMRP from the FMRP-containing mRNP complex, a sequential immunoprecipitation strategy was utilized, which was as described previously (Ceman et al., 2003). Briefly, after immunoprecipitation overnight with 7G1-1, FMRP-containing complex was disrupted and eluted by boiling in SDS sample buffer. The elution volume was then increased with Triton X-100-containing buffer (0.5%) to sequester the SDS and then re-immunoprecipitated with 7G1-1. FMRP was resolved by SDS-PAGE (4–20% gels; BioRad) and analyzed with silver stain (BioRad).
Transfected STEK cells were treated with cytochalasin-D (20 μM), to induce projections in S2 cells as described previously (Ling et al., 2004), fixed, stained with DAPI (Kim et al., 2009) and examined by fluorescence microscopy (Zeiss Axiovert 200 M inverted microscope, 63×). GFP-positive cells that displayed projections but also had no nuclear deformities suggestive of cell death were used for granule counting. Granules were counted using Cell Profiler 2.0 (Carpenter et al., 2006). GFP foci were enhanced using the enhance or suppress features module and the speckles setting. To measure primary objects (granules), the signal intensity threshold was determined using the robust background adaptive method and objects above threshold were identified. A total of 23–26 cells were counted per trial and all experiments were performed in triplicate.
We thank Benjamin Garcia and Geena Skariah for their thoughtful input on this project and Labib Rouhana for critically reading this manuscript. In addition, we would like to thank Lin-Feng Chen for the use of his microscope, Andre Hoogeveen for the KI antibody, Valentina Evdokimova for the antibody to YB1 and Jean-Louis Mandel for the 1C3 antibody. This work was supported by the National Institutes of Health, University of Illinois at Urbana-Champaign Developmental Psychobiology and Neurobiology Training Grant [#2T32HD007333-21 to E.B.] and the Spastic Paralysis Research Foundation of the Illinois-Eastern Iowa District of Kiwanis International [to S.C.]. Deposited in PMC for release after 12 months.
- Accepted June 2, 2011.
- © 2011.


text:
Here is the Bendix link we discussed. This covers compressors, dryers and brake stuff.
https://www.bendix.com/EN-US/SERVICE/LI ... heets.aspx
Here is another good one. This one probably has everything you need for the brake systems.
http://diesel.btc.ctc.edu/Brakes/Bendix ... 4_2002.pdf
This one has good explanation and drawings of a dual brake system.
http://www.mpi.mb.ca/PDFs/DVL_PDFs/AirB ... ction6.pdf


text:
I've got an audio amplifier chip attached to the Rev. 1 ESP32 and I'm trying to get the PDM-mode of the i2s mode running without success so far. There are several things I struggle with and somehow no sound seems to emerge from my speakers, even though I tried multiple different combinations of parameters... I'm trying to get it to work with the i2s driver module i2s.c.
Here's what I do:
First, I initialize the module
This results in the following console output:
Code: Select all
const i2s_pin_config_t pdm_pin_cfg =
.bck_io_num = I2S_PIN_NO_CHANGE,
.ws_io_num = PDM_CLK_PIN,
.data_out_num = PDM_DATA_PIN,
.data_in_num = I2S_PIN_NO_CHANGE
i2s_config_t i2s_config = {
.mode = I2S_MODE_MASTER | I2S_MODE_TX | I2S_MODE_PDM,
.sample_rate = 16000,
.bits_per_sample = I2S_BITS_PER_SAMPLE_16BIT,
.channel_format = I2S_CHANNEL_FMT_RIGHT_LEFT,
.communication_format = I2S_COMM_FORMAT_PCM,
.intr_alloc_flags = ESP_INTR_FLAG_LEVEL1,
.dma_buf_count = 8,
.dma_buf_len = 64,
.use_apll = 1,
ESP_ERROR_CHECK( i2s_driver_install(I2S_PORT, &i2s_config, 0, NULL) );
ESP_ERROR_CHECK( i2s_set_pin(I2S_PORT, &pdm_pin_cfg) );
So... the clock is running at 512kHz? I'm not sure about I2S, but concerning PDM, from my understanding - mainly from the datasheet of the amplifier - the PDM clock frequency for a sampling rate of 16kHz should be at 2.048MHz for 128x oversampled PDM. If I read the chapter 11.4.7 "I2S PDM" of the ESP32 Technical reference manual, if I understand this right, it says that the PDM-Frequency will always be 48x128kHz, i.e. 6.144MHz. That would correspond to an audio sampling rate of 48kHz.
Code: Select all
I2S: APLL: Req RATE: 16000, real rate: 15999.001, BITS: 16, CLKM: 1, BCK: 8, MCLK: 5.208, SCLK: 511968.031250, diva: 1, divb:
Is this correct? Is the PDM mode of the I2S peripheral built such, that it will always output 48kHz audio at a PDM-clock-rate of 6.144MHz? If so, then the frequency calculation for the APLL in i2s.c is definitely wrong!
After the initialisation, I just continuously feed a sine wave. I'm not sure in what format (endianness) it has to be, but as long as I don't hear no sound at all, I don't really care if the PCM data is read out correctly.
I also tried to use it without the audio PLL, but this seems to result in an extremely unstable clock (see trace below)!
Code: Select all
static const uint16_t mod_sin_table_a[] =
32767, 37892, 42892, 47642, 52026, 55936, 59276, 61962,
63930, 65130, 65534, 65130, 63930, 61962, 59276, 55936,
52026, 47642, 42892, 37892, 32766, 27641, 22641, 17891,
13507, 9597, 6257, 3571, 1603, 403, 0, 403,
1603, 3571, 6257, 9597, 13507, 17891, 22641, 27641,
assert(0 < i2s_write_bytes(I2S_PORT, (const char *)mod_sin_table_a, sizeof(mod_sin_table_a), portMAX_DELAY));
I'd be happy for any pointers on what to do to get PDM working! I urgently need it!
EDIT: Removed section about signals being out-of-phase, as that does not seem to be relevant.
EDIT 2: Okay, at least without APLL it seems to be working on the ESP32-side, I do also have a hardware problem here... Nonetheless, a fix for the APLL calculation would be great!


text:
Slideshow: Tips for Contact Lens Wearers at Every Age
Up Next
Next Slideshow Title
Sources | Medically Reviewed on 10/11/2019 Reviewed by Whitney Seltman on October 11, 2019
1) Oleksiy Maksymenko/im / imagebroker
2) Ary6 / E+
3) Klaus Vedfelt / Riser
4) Image Source
5) Van D Bucher / Photo Researchers
6) WIN-Initiative
7) Adam Gault / Caiaimage
8) Tom Grill / Iconica
9) face to face / doc-stock
10) Dan Foy (orangeacid) / Flickr Collection / Getty
11) Henrik Sorensen / Stone
12) Rick Neves / Flickr Collection / Getty
14) emre ogan / E+
15) manley099 / Vetta
All About Vision: "Acanthamoeba Keratitis: What Contact Lens Wearers Need to Know," "Caring for Gas Permeable Contact Lenses," "Caring for Soft Contact Lenses," "Contact Lenses That Enhance Sports Performance," "Contact Lens Basics," "Contact Lenses," "Sunglasses: Frequently Asked Questions," "Teens and Contact Lenses: What Parents Need to Know," "Tips for Contact Lens Wearers," "Tissue-Wiping Your Contact Lens Case May Keep More Bacteria Off Your Lenses."
American Optometric Association: "Contact Lenses and Cosmetics."
Cleveland Clinic: "Soft Contact Lenses."
EyeSmart, American Academy of Ophthalmology: "Computer Use and Eyestrain," "Decorative Contact Lenses," "Using Eye Makeup," "Tips for Choosing the Right Sunglasses."
Reviewed by Whitney Seltman on October 11, 2019
This tool does not provide medical advice. See additional information.
THIS TOOL DOES NOT PROVIDE MEDICAL ADVICE. It is intended for general informational purposes only and does not address individual circumstances. It is not a substitute for professional medical advice, diagnosis or treatment and should not be relied on to make decisions about your health. Never ignore professional medical advice in seeking treatment because of something you have read on the WebMD Site. If you think you may have a medical emergency, immediately call your doctor or dial 911.
WebMD Slideshows
View our slideshows to learn more about your health.
Psoriasis 14 home remedies to try.
Rheumatoid Arthritis Is it the source of your aches?
Foods for Focus Eat these to help you stay on task.
Multiple Sclerosis Warning signs to watch out for.
Attention Killers What steals your concentration?
Cancer Does THIS cause cancer?


text:
Addressing the complex problems of our society (energy, health, and sustainability) will require solutions drawing on knowledge across disciplines. Scientists and engineers trained to utilize multidisciplinary knowledge will be increasingly sought after. My teaching philosophy embraces the burgeoning multidisciplinary approach to science and engineering as part of a chemical engineering curriculum, while maintaining the rigor of the traditional chemical engineering core. I focus on developing students' critical thinking skills by asking them to tackle new problems, that are often open ended, and have no single correct solution.
I believe that by breaking chemical engineering problems into their underlying fundamental phenomena, students can more easily see how to apply fundamental knowledge to other types of problems. In this way, they can make the connections between traditional chemical engineering problems, and problems in emerging multidisciplinary fields.
Modern Biomolecular Engineering (ChE 8450) - Fall 2017
Biomolecular Engineering (BMOLE 4250/6250) - Spring 2015, Spring 2016, Spring 2017, Spring 2018
Mass Transfer & Separation (ChE 3300) - Spring 2013, Spring 2014, Spring 2015
Chemical Reaction Engineering (ChE 4500) - Fall 2013, Fall 2014, Fall 2015
Graduate Reaction Kinetics (ChE 8050) - Fall 2016, Fall 2017
Unit Operations Lab II (ChE 4070) - Fall 2012
Honors Research (ChE 3950/4950) - Fall 2013, Spring 2014, Fall 2014, Spring 2015, Fall 2015, Spring 2016
Creative Inquiry (ChE 1990/2990/3990/4990) - Spring 2013-CurrentMentor Guided Research (CES 1900) - Summer 2014


text:
Southern Fires
575 Bellevue St. (Jefferson Ave.) Send to Phone
313-393-4930 | Make Restaurant Reservations | Menu
OpenLunch & Dinner daily
- Valet parking
- Dress code: Casual
- Kid-friendly
* Click here for rating key
: John Thompson, former co-owner of Franklin Street, has had patrons lining up since the opening of this eatery. The reason? This is soul food done right. Decorated in earthy tones, Southern Fires is huge, with 340 seats on the sprawling first floor of a former warehouse. The menu is simple: five beef, three seafood, two pork and three chicken entrées, no salads and no alcohol. All dishes are executed by chef Beatrice Coleman, using Thompson's recipes. We like the slightly tangy barbecue sauce atop smoky ribs as well as the crunchy fried chicken and country fried steak. Cornbread and two sides like greens, mac 'n' cheese made with four types of cheese, sweeter-than-sweet sweet potatoes and cornbread stuffing come with your meal. Peach cobbler is a good finisher. Plan on using the $2 valet parking, yet another steal. There's carry out and catering as well.
Check GAYOT's list of
Top Thanksgiving Wines.
Wine of the Week Review by GAYOT


text:
Yoga Infographics
There are some wonderful infographics out there which display data in an easy to consume way. We have shared a selection on our website.
Is Yoga For Me
Courtesy of Alison Hinks Yoga Here's a brief rundown of each of the eight limbs: The Yamas - rules of moral code which includes ahimsa (non-violence or non-harming), satya (truthfuless), asteya (non-stealing), bramacharya (sexual restraint) and aparigraha (non-possessiveness). The Niyamas - rules of personal behaviour which includes saucha (purity), santosha (contentment), tapas (discipline or austerity), Read More
Vinyasa or flow yoga is when you synchronize your movements to your breath, and the moves all flow together. Breathing is very important, because you must move from one pose to the next on an inhale or an exhale through the nose. Sun salutations is an important part of the Vinyasa yoga practice. Firstly, it can be a great Read More
You want to take up yoga but are not sure which one would suit you. Try out this little quiz to see which type of yoga would suit you with regards to what you would like to achieve with Yoga. Infographic courtesy of Alison Hinks Yoga


text:
Meghalaya Results 2017
Meghalaya Results 2017: Candidates can check all Meghalaya Results 2017 through this page. The results are declared only for those candidates who have appeared in the exam. Students can check various examination results Meghalaya TET Results in 2017 on this page. So the students are advice to check latest updates regarding your results here. Students can bookmark allindiaresult.net and visit it regularly to get all the latest news updates about Meghalaya Results. Every board has different rules and regulation. Based on these rules and regulation, boards of education in India declare the results. Students want a perfect and accurate place from where they can able to get the latest updates and information about the Meghalaya Results 2017.
|Name of The Exam
|Exam Wise Results Links
|Meg TET 2017/Meghalaya TET 2017/ML TET Exam 2017
Meghalaya Board of School Education
|Meghalaya TET Result
|JEE Main Exam 2017
IIT-JEE Main Examination Board
|JEE Main Result
|GATE Exam 2017
IIT Roorkee
|GATE Result
|National Eligibility Entrance Test 2017
|NEET Result
Meghalaya Results
The Meghalaya TET exam is known as ML TET. The exam guidelines are maintained by the central regulating authority. The examination has two categories. One can either apply any one of them. The first paper is targeted for the primary section that is the individual has to teach classes one to five. The second paper is for the secondary section that is from classes six to eight. ML TET 2017 notification will be released by the Department of School Education, Meghalaya OR concerned examination board as directed by the Government of Meghalaya. TET is a compulsory examination for all the B.Ed. qualified candidates to be considered for teacher jobs in schools run by the government and private managements. Candidates may be facilitated to apply online for this examination.
MBOSE SSLC Results 2017
The Meghalaya Board of Secondary Education (MBOSE) has successfully conducted the Secondary School Leaving Certificate (SSLC) Examination for the session year 2016-17 in the Month of March/April. Now the MBOSE is going to declare the Meghalaya Board SSLC Result 2017 in the Month of May. The Students who are searching for their MBOSE 10th Class Result 2017, they can check their Tenth Exam Results from below provide a link. The Meghalaya Board of Secondary Education will upload the Meghalaya Board SSLC Result 2017 only on its official website. The board is now busy outlining the timetable for the SSLC Public exams. Students need to come to terms and should start preparing for the exams so that there will be time left for revision when the exams are nearer. The board will held exams for all the students of the state who study in the schools that are recognized by the board.
MBOSE HSSLC Results 2017
Meghalaya Board of Secondary Education is all set to declare the Meghalaya HSSLC 12th Results in the State in the month of May.The Board is going to announce the Meghalaya Class 12 Result at the end of the month May 2017.The Board has conducted the Class 12 Examinations for all the 1st and 2nd-year students in different streams such as Science, Commerce, and Arts.The Exams of 1st year and 2nd-year Intermediate students were held on alternative days in the month of March. The Meghalaya Board has conducted HSSLC Exam in the month of March/April. So students who had appeared in the examination and waiting for the result can check Meghalaya HSSLC Result 2017 by using their roll no wise or name wise from the official website. Large numbers of candidates appeared in HSSLC main examination. Now all those candidates eagerly waiting MBOSE HSSLC Result 2017, they can check the official website.
Meghalaya Results Information
The examinations are usually conducted in the month of February or March, and Results will be announced in the month of May or June. So the students are advice to check latest updates regarding your results here. We will provide information on Results Meghalaya 10th Results 2017, Meghalaya 10th Class Results 2017, MBOSE 10th Results 2017, Meghalaya 12th Results 2017, MBOSE 12th Results 2017, MBOSE HSSLC Results 2017, Meghalaya State Matric 10 Results, Meghalaya Board Senior 12th Results, Meghalaya Board 12 2017 Results, MBOSE SSLC Results 2017, Meghalaya Board 10 2017 Results, Meghalaya MBOSE SSLC/HSSLC 10th/12th. For more information or details about Meghalaya Education Board, then please visit the official website of the Meghalaya Board.


text:
Hole 16 - Cottons
What a magnificent golf hole. Visually this hole can be both very beautiful and quite intimidating at the same time. It is another short par 4 which asks a question. The 16th dog legs quite severely from left to right, playing around a bend in the river Tame and to a green surrounded by water on three sides at the end of a small peninsula.
To view the green from the tee, just look directly along the river and there is the flag. If you love golf, and enjoy standing on a tee which makes you smile and shake your head at the same time, you need to play this one.
The more you cut off the dog leg by aiming further to the right, the narrower the fairway becomes. This hole demands an accurate tee shot. Unless you aim way left off the tee, you must play over the out of bounds so take enough club to clear it. The safest shot is down the left side to leave a mid to short pitch on.
Named after Henry Cotton who described the 16th hole as 'the best looking, most fair and at the same time probably the most difficult hole I have ever played'.


text:
PCTEST will be attending the TCB Council Workshop in Baltimore Maryland.
From TCB Council's website:
The TCB Council is pleased to host our April 2016 Workshop from April 12-14, 2016 at the Holiday Inn – Inner Harbor in Baltimore, Maryland. This Workshop, like recent April Workshops, will be divided into a one-day Basic Training on April 11, geared toward training the new personnel in our industry. This will be followed by our regular 3-day Workshop on April 12-14, 2016. The Education Committee has reviewed past Workshop evaluation/comments to build an excellent agenda for these Workshops.
Click here for access to the detailed information about the Workshop, Agenda and hotel information.


text:
StatsServices: Escort Service, Massage, Fetish & Role Play, GFE, Incall, Outcall
Locations: Oakland, San Francisco, San Jose
About me
Is personality important to you? It is to me. I pride myself on being the most down to earth and friendly person you will ever meet. You will find that I am intelligent, playful, classy and compassionate. I love to spoil the man I'm with. I have a very wild side when it comes to pleasing my partner. I strive to please you on many levels. I ooze sex-appeal. Let me find out what you like and GIVE IT TO YOU!
I look exactly like my photos. What you see is what you get. I have a flawless body.
I am always on time and discreet, during our time together you will always have my undivided attention.
Are you ready to meet me yet? Still need to know more about what I offer? If below sounds like what you are looking for then call me.
If you have accomplished everything you set out for, yet you are still needing something, I am what you are missing. Let me be your girlfriend for the evening and help you fulfill your every fantasy. If life has left you feeling taken for granted and unappreciated, I can show you what feeling alive and wanted is all about. Starting a whole new life is just not possible for everyone and some just have to much invested. With me, you can keep all you have and add the excitement you had for life and the dreams that made you alive.
For a date you will always remember and a friendship with new beginnings let's make a date.


text:
Ephemeral bookstore
26 - 30 SEPT, 12 H - 20 H, Quartiers POP & Espace POP
éclair is a moving micro bookshop and literary consultancy based in Montreal. They build small collections of unique, strange, and worthy books circling around a particular theme. For this year's Art POP, they have put together an exciting collection of picks that include fiction, theory, poetry, comics, zines, artist publications, and children's books, available in English and French. We want you to discover books that hit like lightning bolts!
Find éclair at various locations throughout the festival.


text:
Linda, by birth, my mother is/was Italian and my father Jewish (Russian). My father had blond hair and blue eyes, my mother very dark brown hair and brown eyes. When you see me, you will not believe it. I grew up as a "tow-headed" blond with chameleon eyes (change from grey to blue to turquoise). Now, I am a washed-out blond with blond highlighting, as I am not turning grey fast enough.Donna
Folders |
Best Of |
Favorites & Replies |
Settings |
Start a New Board |
My Fool |
Help |


text:
Our Dermatologist specialists treat all disorders of the skin, including rashes, pigmented lesions, acne and skin cancer. They have over 30 years combined experience in Dermatology. While our department is exclusive in Dermatology - handling only medical issues of the skin - because we are part of Optum, we also have over 70 other physicians in many specialties readily available to help our patients with surgical or other special needs.
The following links also provide additional resources you may find helpful:
American Academy of Dermatology
American College of Mohs Surgery


text:
Smiley Puzzle Girl Edition
Tags: Puzzle Games / Girl Games
Publisher: SmileyGamer.com
Video Walkthrough
Smiley Puzzle Girl Edition is a Puzzle Game where you need to drag columns and rows to make matches of 3 or more smileys. The more smileys in a match, the more points you will get! Some smileys will prevent you from dragging the column and row they are on. Watch out for the special gloves that let you erase a single smiley or recolor it!
· Controls = Use The Mouse
Embed this game
Share this game


text:
What is your proudest Citizens moment?
Witnessing important issues, being presented by normal people (citizens) working together toward a common goal and achieving it. I observed stake holders including the city councillors from all different political parties and MPs agree to the people's requests for accepting more refugees, to sign up to Living Wage and more. The most recent proud moment was being part of the team that planned the Great Get Together Iftaar on 16th June.
What's the impact of membership been on you and your institution?
This year, our community took part in various events and actions. We developed strong links and relationships, both personal and as an organisation., friendships with people from all walks of life and from many local and national organisations. We achieved exposure for our work and goals, and found common ground, values, aims and passions, shared with many in our wider MK community.
What are you most looking forward to over the next 12 months?
I am looking forward to the next task; planning and organising a Fight Against Hate summit during national Hate Crime Awareness Week which will be mid-October. We will be supporting Citizens:mk's Fight Against Hate campaign team. As a community we are looking forward to achieving more, grabbing opportunities, developing and making greater impact in our society under the Citizens:mk umbrella.


text:
June 23, 2011
Finding Motivation to Control Spending
In a May 5 Squared Away blog post, I provided a list of financial planners' five favorite tools for helping people control their spending. In this post, I'm providing their motivational suggestions.
Here are the five tips, based on my informal survey of planners. Each tip includes the psychological rationale behind it.
Find the "Aha! Moment."
Some clients respond when they see, in detail, how much they're spending and what they're buying. Bonnie A. Hughes, a northern Virginia planner, is a big believer in mild shock therapy. She's had great success by showing clients how much their income would fall if they were laid off, divorced, or dropped out of the workforce. Or she shows them just how much they'll need in retirement, and it's usually a big number.
Reason: The Aha! Moment provides the self-motivation that clients must possess and that planners can't provide. …Learn More


text:
What we're looking at today is the handset version of the rather impressive News360 news feed app that's been previously available for Honeycomb-laden Android tablets. We've got a review of that available for you as well, of course, because we love to grab those fabulous app reviews as soon as possible, and now here's the BRAND NEW handset version, fresh as can be! What this handset version does is take the same beautiful aesthetics and a very similar user interface as the original tablet-based newsfeed app and applies it to the smaller-screen having handsets you use on the daily. Will it add up? We'll see!
In the video below you'll get a glimpse of the tablet version of this app, for a full look at it, head over to the June 15th 2011 App of the Week post. That's right, this app is so good, it's gotten that title twice! What this new version does is take the same excellent content they've got streaming to you over your internet connection and pushes it down to a single image, title, time, date, and source. That's the most basic view. You'll be flipping back and forth, seeing the main image and titles that interest you, searching until you find the story that strikes your interest the most. At this point you'll just want to tap the image (or the title) and you'll be brought into a single text-based article view.
Article View
Inside this individual story mode, you'll be able to see a shortened version of the story you're interested in along with several options for reading. First, you'll be able to switch on or off highlighted keywords. When you tap a highlighted keyword, you'll be brought to a screen that identifies the keyword, this also linking you to stories that use that keyword prevalently. Next you'll be able to adjust text size (for people with small eyeballs.) Third, you'll be able to share the article using several social networks and sharing programs.
News360 has a special screen with Facebook, Twitter, ReadItLater, Instapaper, and other – other leads you to your normal share screen where you'll have the option to share with all the apps you'd normally be able to share with on your handset, including, in our case right this moment, Bluetooth, Gmail, GroupMe, and Mail. What's currently missing? Google+ of course! Lastly you'll be able to hit a Star which will add the article to your favorites for later access. You can also head below for a link to the full article on the source's webpage. This source link does not lead you outside the app into a browser app, instead conveniently sticking inside the app's own browser window for ease of continued access.
Below this is a set of "more coverage" articles that connects to the same story as written by different sources. Strange and awesome, that's what we're thinking here.
Top Stories
On your main screen in News360 there's a tiny bar link at the bottom of the screen that reads "Top Stories." It's here that you'll be able to get into your list of selected topics that will then keep you in the same single-story-title-and-picture mode but will have adjusted the subject matter to the keyword of your choice. Elegant and simple solution for a tiny screen!
Local Filter
One of the coolest features from the tablet version is still present here: the target button that leads you to a filtered collection of stories. One thing that us modern generation internet-based folk have trouble with (or, at least, quite a few folks my age in my area have trouble with,) is caring about local news when there's a whole internet of world-wide news out there at our fingertips. What this single button does is redirect all of our eyes back down to our own streets – a bonus tip: should you decide to want news from a different area specifically, head to your settings and switch off GPS – you'll be given the option to add a custom location, anywhere you want in the whole entire world! Don't expect too many stories out of Abu-Doobie though, not many online sources in the area.
Search and Favorites
You can search articles and you can keep a list of favorite articles which you can then later read, keep forever, or delete upon not caring once you realize you didn't want to save at all. Helpful and excellent.
Review and Hands-On Video
[vms 64e9f27197a484f4257c]
What's Missing
This version of News360 isn't quite as intricate and full as the tablet version, but that's to be expected. If you get the chance to check out the tablet version, I recommend doing so, as the floating gallery view alone is worth the trip down to your local Android Market!
Where do I get it?
To grab this app for FREE right now, head over to the [Android Market page for News360 for Phones!] It's one fantastic deal – free, all you could ever ask for. Now go in there and find you some Android Community posts so we can be more prevalent amongst the big gun non-Android news teams! Go get em!


text:
One of the least often heard, but most compelling rumors of the past year has been Toyota's development of an eight-speed transmission for its Lexus models. It seems insane. One minute we're in high school, trying to un-jam (with a sledgehammer) the three-speed manual linkage on our ex-fleet 1973 Buick Century, the next we're cruising along in a Maserati, six-speed robotic manual churning away like a coal shoveler on the steamboat Robert E. Lee. Next, we'll be cruising the interstate in some V8-powered Lexus, in some ungodly gear 8, watching our gas-gauge needle stay put, mile on mile. Is it good thing?
The Detroit News offers a quick profile on automakers' efforts to add additional tranny gears, which is becoming a competitive struggle of big-block proportion. Next model year, Mercedes will offer the company's new 7-TRONIC system, and VW already offers a six-speed automatic on its 2006 Jetta. Even slowpoke GM has entered a partnership with Ford to (finally) develop a six-speed autobox, of which the company expects to sell three million per year by 2008. Of course, we should be happy mechanical engineers' degrees aren't going to waste, considering such multigear slushboxes, with the help of today's sophisticated control software, continue to wring better fuel efficiency out of the most powerful engines.
Gear wars: The race for an eight-speed car [The Detroit News]


text:
Gojek is a Super App. It's one app for ordering food, commuting, digital payments, shopping, hyper-local delivery, getting a massage and two dozen services. It is Indonesia's first and fastest growing unicorn building an on-demand empire across Southeast Asia.
As of 2018, Gojek processed more than $9 billion annualized gross transaction value (GTV) across all markets where it operates - in Singapore, Thailand and Vietnam. The Gojek app was launched in 2015, and is now the largest consumer transactional technology group on a GTV-basis in Southeast Asia.


text:
Bob Wilson
Bob Wilson Footballer - Born in Chesterfield 1942.
Signed for Arsenal in 1963 and made more than 300 first team appearances.
Career in sports journalism started with the BBC. He presented Football Focus for 20 years and was also a regular on Grandstand, Sportsnight and Breakfast sport before being lured to ITV in 1994 to be the channel's main presenter.
Bob ran his own goalkeeping school for youngsters for 13 year. David Seaman was one of those to benefit from the Wilson system.
Services to football which have included writing several books on the subject have brought him academic honours including honorary doctorates from the universities of Derby and Middlesex. Now lives in Herefordshire with wife Meg, and runs the Willow Foundation, a Charity set up in the memory of his daughter who died of cancer at the age of 32.


text:
Product Code: 73369
Shop all ANUSOL products now!
Roll over image to zoom in
Hemorrhoidal Suppositories Regular Pain Relief 24 Tablets
AvailabilityIN STOCK
Anusol Suppositories eases the itching and burning sensation caused by hemorrhoids. It gives you fast acting, long-lasting relief so you can go through your day pain free.
- Zinc sulfate monohydrate suppositories
- Fast, long-lasting relief
- Helps reduce swelling
- Doctor and pharmacist recommended


text:
boen_robot 107 Posted March 13, 2007 Report Share Posted March 13, 2007 I can tell you the benefits of using XSLT over pure ASP.NET right now. It's future proof. What if tomorrow MS decided to deprecate support for their sitemap objects? What if you decided to use PHP at some point?Also, I don't know about you, but to me, XSLT feels a lot more readable. At least it's pure markup.As for your problem... not having the complete site map file here is probably what confuses me, so I'll work with what I have. You mean that if aboutus.xml had: <aboutus><internal_links><link>default</link><link>arealinks</link><link>faqs</link><link>contactus</link></internal_links></aboutus> (notice my addition)You would want to get the links in the sitemap file that correspond to those keywords (if any) by using the value and ID as a key? How about this: <xsl:variable name="uri" select="'web.sitemap'"/><xsl:for-each select="aboutus/internal_link">
<xsl:for-each select="link"><xsl:varible name="cur" select="."/>
<xsl:value-of select="document($uri)//siteMapNode[@id = $cur]/@title" />: <xsl:value-of select="document($uri)//siteMapNode[@id = $cur]/@url" />
</xsl:for-each>
</xsl:for-each> By the way, as far as I've heared, xsl:key is a more efficient method for this. However, I don't have enough experience with it to show you. Link to post Share on other sites


text:
Smart entrepreneurs would rather buy than make, whenever doing so gives their customers the best deal. From the internal entrepreneur's point of view, every outsourcing contractor and vendor is considered a resource, not a competitor.
In an extended-staffing strategy, external service providers are treated as part of the internal group that offers the same skills and products.
To make extended staffing work effectively, every internal entrepreneur cultivates contacts with outside vendors and contractors within his or her area of expertise. Standing contracts may be arranged to permit rapid acquisition of help when demands warrant. In fact, regular contractors may even be pre-trained in the firm's technologies and business processes so that they're prepared to go right to work when needed.
Whenever peaks in demand occur, entrepreneurs acquire resources (funded by their customers) rather than lose the business. This way, they are never resource constrained. The only limit to an entrepreneur's capabilities is the customers' ability to pay for the work they need.
Staff doesn\'t limit its use of vendors and contractors to peaks in demand. In the interest of offering their customers the best possible deal and maintaining their preferred-vendor status, internal entrepreneurs should be the first to offer vendor products as alternatives to internally-produced products and services.
Of course, since vendors and contractors are extensions of their staff, internal service organizations add value by carefully selecting suppliers. They\'re the ones who are the best qualified to judge the competence of vendors in their field of expertise. And they know that their reputation rides on the success of their key suppliers.
An important consideration in selecting a vendor is deciding the most appropriate type of relationship (to be explored in a future column!). Each type of relationship has its advantages and risks. Internal staff adds value by selecting the right relationship.
Once hired, entrepreneurs manage vendors and contractors as if they were part of the group. Corporate boundaries are irrelevant once the purchase decision is made. Internal staff assigns tasks, manages projects to internal quality standards, and retains accountability for the vendor's work. (In government, this is done through management of statements of work, not personal service contracts or day-to-day management of contractors; but the point remains.)
From the clients' points of view, outside contractors appear the same as their internal counterparts. The fact that their paycheck is written by a different company doesn\'t change the nature of their work or their relationships with others on project teams.
Project funding must be sufficient to pay for the added value of internal staff managing contractors. In other words, when bidding projects that will utilize vendors and contractors, entrepreneurs add a small mark-up to the external expenses to cover their own time. They use this extra money to buy a bit of extra help from contractors, whom they use to off-load internal staff from other projects so that they can manage the contractors.
In fact, once a client agrees to fund a project, staff can use their money in a variety of ways to match project requirements with resources. Internal staff should focus on projects that require their unique skills and knowledge. Staff should also be assigned projects which provide learning opportunities that will benefit their careers, while contractors should be given the more mundane tasks.
What if a new project is most appropriate for internal staff, but everybody is busy with other assignments? Clients generally do not care who does the work, as long as someone they know and trust manages both projects and the promised deliverables are achieved. Staff can use the funds provided to do the new project to hire contractors, and then assign the contractors to other projects to offload staff to do the new project. This ensures an optimal use of internal and external resources.
All of the right things will happen, automatically day after day, if staff develops an entrepreneurial culture that proactively bids every deal and manages vendors as part of their staff.
As I mentioned earlier, when acquiring help from a vendor, the firm must make a decision about the type of customer-supplier relationship. Extended staffing alternatives include the following four types of relationships, each with advantages and risks:
- Product vendors.
- Temporary employees.
- Project employees.
- Long-term contract employees ("perma-temps").
In the next column, I\'ll discuss when, depending on circumstances, each of these relationships might be optimal.
Useful Links:
Learn more about the Òentrepreneurial cultureÓ here:
4 Dimensions To Managing Your Service Provider
4 Advantages to Outsourcing
How Executives Paralyze the Effectiveness of Their IT Teams
Wresting Control of Priorities from Your Internal IT Team
Why Your IT Team Needs a Full-time Internal Consultant
How To Get Your IT Staff to Give You Service Like Your Service Provider


text:
- Join over 1.2 million students every month
- Accelerate your learning by 29%
- Unlimited access from just £6.99 per month
Extracts from this document...
THE WALL STREET CRASH AND THE GREAT DEPRESSION Q1: The stock market collapsed dramatically in October 1929. Why did this happen? Ans: The period in America between 1920 and 1930 was known as the 'roaring twenties'. This term was applied to the US because of the economic boom in the country. The World War I ended in 1918 after which there was a great change in the American society. The mindset of Americans changed greatly during the post-war period and thus began a new age of possibilities. Everybody in America wanted a bright future for themselves and their families and so they began investing in the American stock market. Industries at that time in America were booming because of new technologies, increased man-power and a promise of prosperity for the investors. People had a high rise in income during this period. Therefore, they were convinced that they would come out richer if they invested their money in the stock market. There were a number of reasons for this new trend and spending habits of people. 1920's was a period of industrialization. Productivity in industries increased greatly during the roaring twenties. There were new methods of production that helped step-up production and made it safer. The ford car company was one such industry that prospered during the boom. They made cars using the assembly-line production and thus the speed increased. In 1900, only 4000 cars were being produced and the production increased rapidly over the years. In 1929, 4.8 million cars were being made. There was a low rate of unemployment because as the industries grew, manpower needed also increased. The rate of unemployment decreased from 5 million in 1921 to only 2.5 million in 1929. The government also aided America in this rapid growth. They cut down taxes on imports and exports due to which trade boomed. Within the country it helped the richer section of society who bought land and assets because of which the economy would get a boost. ...read more.
Just some days before the Wall Street crash, over-confident investors such as Charles E Mitchell said, "the industrial condition of the United States is absolutely sound." Another learned professor of Economics at the Yale University, Irvin Fisher stated, "Stock prices have reached what it looks like a permanently high plateau." This over-confidence was contrasted by a sudden selling of shares on 29th October that was the day of the crash. The crash was followed by a period of the great depression. The period between 1920 and 1929 was known as the 'roaring twenties' in America. The lifestyle of people had changed a lot during these years. As their income increased, their spending habits also changed. During these years, about one-third of the American population was investing in the American stock exchange. This is why they had a lot of disposable income. They invested in any company that they found promising. As a result, industries in America started producing goods in huge quantities. There were new technologies introduced because of which production grew rapidly. People bought the latest products by taking loans that were given very easily by banks. The system of credit cards was also introduced during this period of prosperity. The term 'buy now, pay later' became common amongst commoners. They would buy goods on credit and paid through instalment. However, when the depression began in America, people were forced to repay their loans and banks also started shutting down. Big industries had over produced according to demands which had decreased towards 1928. This was because people had already bought whatever they could afford. The surplus could not be exported to other countries because the prices of American goods were so high that Europeans couldn't afford them and when the excess wasn't sold, the companies began producing less. As a result, share prices started falling and investors lost interest in companies. These were the short-term causes that led to the great depression after the Wall Street crash. ...read more.
They would sleep in parks at night or build themselves untidy shanties. The effect of depression on children was quite different from what it was on elders. They took on responsibilities at an earlier age and tried to find jobs to support their families. A 12 year old boy in Chicago wrote to the president asking for help because he saw his father weeping and felt sorry for him. Thus children behaved more maturely and helped their families. The overall condition in America was bad because the economy had collapsed more than 60% of the population did not have money for their basic needs. The president of America, Herbert Hoover reassured people that the country would get back to its feet again and rise to prosperity. He cut down taxes even further and this encouraged people to buy more goods. By mid 1931 there was an increase in production and again it looked as if America was settling back again. However, the national income of the country fell from $59 billion to $42 billion by 1932. Important individuals such as Irving Fisher had stated that market would get stronger but just three days later it collapsed. He was left poor just as other Americans and people blamed him for his wrong forecasts. Charles E Mitchell, who was also an affluent American, was also left poor by the crash. He had bought shares when people were desperately selling them and finally he too ran out of money. He was also arrested for tax evasion during the depression period but eventually paid his debt by 1938. These were the harsh conditions that the Americans had to live with during the days of the great depression. Not only America but even countries such as Germany and Britain faced the slump. Unemployment was about 6 million in Germany and 3 million in Britain. Lives of people had changed greatly before America could recover from the terrible days of the depression. Sources: www.en ?? ?? ?? ?? History Coursework-1 Devanshi Sodhani IGCSE May 2008 Centre No. IN-405 Candidate No. 0293 1 ...read more.
This student written piece of work is one of many that can be found in our GCSE History Projects section.
Found what you're looking for?
- Start learning 29% faster today
- 150,000+ documents available
- Just £6.99 a month
- Join over 1.2 million students every month
- Accelerate your learning by 29%
- Unlimited access from just £6.99 per month


text:
Breakthrough: How To Get Unstuck With God's Help
5 Days
Have you ever thought, "I need a breakthrough!" Did you know that God is called "God of the breakthrough"? Despite that, many of us live lives that are "stuck" in some area: relationships, career, spiritual growth, finances. While there's no promise that our desires ever be fulfilled, we can get "unstuck." This is not about better goal setting or higher productivity. It's about allowing God breakthrough in our lives that tears down barriers and enables freedom through the Gospel.
We would like to thank Ignite SF for providing this plan.About The Publisher
Related Plans
See All


text:
Having missed the chance to work in the financial markets (usually through a lack of ability and/or qualifications) some beginners imagine that sports trading will provide them with riches without the hard work. Buchdahl shows in S&S, S&S that whilst most don't make a profit, those that do, put in a lot of hard work and are unlikely to hand their edge on a plate to others. I recommend that you read the book before going any further.
If Trader247 was a quant in the financial markets, his manager would be throwing a huge bonus at him, such is Trader247's skill at picking up pennies in front of a steamroller as effectively as any high-frequency trading firm. Only the paucity of volume and market capacity prevents Trader247 from earning more. He has found his technical trading niche and I applaud him for that. I doubt his detractors make a single penny.
This website gets more page views per day than most of its ilk so I must be doing something right. Curiously, outside of the UK the majority of my readers are from Russia and the United States, which are not countries I would immediately associate with the exchange trading of sports. Thanks to Google Translate I can read what my Russian cousins are saying. All good, I am happy to say. I have always thought that the Russians are a highly educated and intellectual people. Contact me for speaking engagements. I hear Saint Petersberg is nice.
The year 2017 will see me releasing more research. Again, no edge given, just tools for readers to do with as they please. My desire is to flood the exchanges with a new breed of algorithmic bot trader, who may have new ideas and new ways of looking at the trading problem and will take trading to the logical conclusion that I, Buchdahl and others have come to. Fuddy-duddies pushing tired old manual trading techniques can expect to be hounded again, throughout the year.
Predictions? Andy Murray to retain his number one position in the rankings and to win the French Open. With cycling's Grand Tours reducing team sizes to eight riders and Nairo Quintana still improving I think he could well win the Tour de France in 2017. And, more manual traders will turn to algorithmic trading in 2017 to avoid emotional, tired, loss-chasing, miss-timed trades and to take advantage of speed of data acquisition, processing and trading thus freeing up more time to enjoy life away from their monitor screens.
"Where could you get money that you don't give back? Let's go on with the show!"
(Ethel Merman - and in photo)


text:
By Jennie L. Phipps for Bankrate.com
First-time parents who are in their 40s and 50s face some retirement-planning issues that are different from those generally encountered by people who got an earlier start on rearing children.
A baby shower of advice
Put saving for your retirement first. There is a very real possibility that you'll be paying for your child's college education at the same time that you're considering hanging up your work boots. Sheila Gugliuzza, managing director, wealth management for TIAA-CREF, advises that you put retirement savings ahead of other savings because you can't borrow money for your retirement.
Find out if your child will be eligible for Social Security. Once you hit Social Security's full retirement age, your children who are younger than 18 (or 19 if they are still in high school) are eligible for up to half of your benefit. This applies to natural, adopted and dependent stepchildren. A spouse who is too young to claim benefits on his or her own and who is caring for a child can also qualify.
For instance, if you were receiving $1,500 a month and you had a spouse and two children, each could receive $750 a month. There is a family maximum of 150 percent to 180 percent of the Social Security participant's full benefit. Your own Social Security benefit won't be reduced, and there are no income limits. Some people have dubbed this the "Viagra benefit," and some of them are reluctant to take it. Gugliuzza says, don't be shy. "When Social Security is available it definitely makes sense for people to take it."
Invest in disability insurance. It is smart to buy a robust, long-term disability insurance plan. The Insurance Information Institute, a trade group, estimated in 2009 that a 40-year-old has a 43 percent chance of being disabled for more than 90 days by the time he turns 65.
Don't neglect life insurance. Gugliuzza recommends that you consider buying insurance on your life in the amount of $250,000 for each child you have at a minimum.
Identify a parenting plan. Name a guardian for your child in case you are incapacitated and can't provide care. Gugliuzza says you might consider picking two people -- "a guardian to provide loving care and a financially savvy trustee to watch over the money."
Get help preparing an estate plan. A simple will often isn't enough to make sure that what you have accumulated goes to your child in the most efficient and beneficial way, says Gugliuzza.
This article was originally published on Bankrate.com.
The views and opinions expressed herein are the views and opinions of the author and do not necessarily reflect those of Nasdaq, Inc.
The views and opinions expressed herein are the views and opinions of the author and do not necessarily reflect those of Nasdaq, Inc.


text:
Compliance Hotline: Online or 1.800.324.5794
Media & News | Jobs | Families | Get Involved | Contact Us
Since opening its first prevention program in Sullivan County in 1990, NYSAP has expanded in both its diversity of programming and geography. Currently, NYSAP provides services across 20 counties from the border of Canada (Clinton, St. Lawrence, Jefferson and Franklin) to the more populous areas of Orange and Westchester Counties as well as our most recent expansion into the Bronx in New York City.
From our 16 offices dispersed throughout NYS, we provide an array of services tailored to meet community-specific needs including:
New York State Brochure


text:
Professor Guy Claxton is an internationally acclaimed, writer, consultant, lecturer and academic, specialising in creativity, education and the mind. He is Visiting Professor of Learning Sciences at the University of Bristol's Graduate School of Education, and a Fellow of the British Psychological Society.
He has written extensively on subjects from creative thinking to Buddhism, and the value of uncertainty in learning and what makes people effective lifelong learners.
As an academic, Guy is the author of a dozen influential books on the mind, including Hare Brain, Tortoise Mind: Why Intelligence Increases when You Think Less, Wise Up: The Challenge of Lifelong Learning and The Wayward Mind: An Intimate History of the Unconscious.
Guy is the UK's leading expert on practical ways of developing young people's learning and creative capacities. As a featured speaker at the South Australian learning to learn expo in 2007, Guy shared his passion for building learning power in the classroom and more recently with learning to learn phase IV sites as part of the core learning program.
Building Learning Power (BLP) written by Guy for teachers explains how to help students become better learners, both in school and out. BLP embodies what he calls the 4th generation approach to learning to learn. It doesn't just add a few tips and techniques, it systematically helps students develop the general habits of mind that underpin confident, curious, creative learning.
During 2008/09 Guy will provide critical support to learning to learn as the South Australian teaching for effective learning compass is further developed.
- Expanding the capacity to learn: a new end for education? (PDF 282KB)
- Improving student's learning (PDF 139KB)
- School as an epistemic apprenticeship: the case of building learning power (PDF 307KB)
- The virtues of uncertainty: a character curriculum for the learning age (PDF 30KB)
- Building learning power: education for a learning life (PDF 910KB)
- Leading the learning school: building learning power in action (PDF 557KB)
Teaching for Effective Learning
Phone: 8463 5959
Email: Education.TfEL [at] sa.gov.au


text:
How sports marketers can hold attention of 'Generation HD'
The latest of these market segments is what has been called Generation HD. This does not reference high definition, nor have anything to do with enhanced viewing or listening to video or audio. Generation HD is shorthand for Generation Heads Down: With eyes glued to their smartphone device wherever they go, this generation is hard to miss.
Generation HD has already developed a set of characteristics that we have observed in sports marketing. First, they are steadfastly keeping their eyes down, consumed in their own personal entertainment, while video screens as long as 60 yards are showing sponsored replays or other messaging. Second, while standing in any line to purchase anything in-stadium, again, heads are firmly in downward gaze looking to see who in their social graph has checked in and announced their entry to the event. Finally, while the venue video and audio is running a broad-reach message, targeted to no one in particular, Generation HD in the audience has tuned that out so that they can focus on the targeted message, delivered via their social network, on their personal mobile device.
With all of the lights, sound and video blasting around and at them during an event, you would not think that someone can effectively tune that out, but Generation HD has. And, the reason that they can is because they always have. For Generation HD, the second screen has become their first screen.
|Teams must give the "Generation Heads Down" crowd a reason to look up.
In order to get eyes up from Generation HD, sports marketers are going to have to consider three important principles:
1. Start earlier.
2. Work harder.
3. Give them more.
In classic brand marketing terms, we have been taught to meet the audience at their level of expectation, and if their expectation is more than the dull, broad-reach audience entertainment of their parents' generation, then we have no choice but to get more social, more mobile, more personal, and more inclusive. Brands and experiences can attract Generation HD sports fans; they just have to have a well-thought-out strategy.
Start earlier
It's no longer satisfactory to start connecting with this audience when they sit down. There are enough information nodes available now that brands can provide data and information relative to products and services in the area of the venue, in the city on the way to the event. This can be about sports (about football, basketball, etc.) or about the social and entertainment opportunities within a mile to 10 miles away. Brands and properties can meet Generation HD at their level of expectation, enhancing their lifestyle. And when these messages are optimized for their social graph and for their mobile devices, sports marketers will get more of their attention and get those eyes up when they need to.
Work harder
Marketers must work harder at defining their offerings and defining a product or event along the lines of the world that these consumers live in, not the one that the marketers work in. For example, Cuban may want to get more eyes up at Mavericks games, but in order to do that, he may have to connect that heads-up activity to something or someone in the heads-down world. Is there, perhaps, an incentive to pay attention to the big board because of what a fan just saw on his or her personal mobile device? If game activities are competing for the attention of Generation HD, brands and properties are likely to lose that battle. Marketers that connect them, or make them sync with a team's programming, will force those eyes up and keep them up longer.
Fifteen years ago, automakers began to understand that unless they could sync in-car entertainment components to a consumer's personal devices they would lose out to those that could integrate effectively. Sports marketers may be faced with a similar dilemma in terms of the big video screens at venues. Could those go the way of AM radio? Not likely, but unless they become more relevant to the fans' own personal experience, there is a risk.
Give them more
For Generation HD, it's not less is more, it's more is more. Generation HD wants more of a relationship with a brand, more acknowledgement of their following of that brand and more transparency into the brand's values, not just the product or the team roster. Crowdsourcing for their ideas is old news to Gen HD. They are going to be excited when marketers reach out to them with ideas for ways in which they can be included in the message, such as featuring them in media or product announcements.
Gen HD is also among the first of the "global kids," raised with a wider world view, social consciousness and values for sustainability, diversity and equality, which are important to them. A brand's engagement in topics such as the environment, social justice, or LGBT, for example, is meaningful to Generation HD. Where do those issues have an ascending presence in a sports brand's mission? If they don't, then the brand may continue to see those heads down, relative to its end goal of reaching Generation HD.
Marketers must listen, learn and understand how to connect and communicate to and with the Generation HD market segment. The long-term success of their sports property, product or brand may depend on it.
Marty Conway is an adjunct professor of sports management at Georgetown University and senior consultant for Way Forward Associates. Follow him on Twitter @MartyConway.