text:
From a young age I had a strong attachment to music. I was pretty confident that music had a unique role in my life. In order to utilize my passion with music I started working as a DJ from a very young age and experimented with mainstream sounds of a wide variety of music genres. Both my personal and professional life is devoted to explore all the aspects and trends of music and to be able to create, and combine sounds so that people can enjoy my set.
In other words music defined me both professionally and personally. I've played in all sort of venues, and touring around mainland Greece and its islands, as well as London, New York, Egypt, Turkey and Cyprus.
Alexandros Christopoulos ★ Compiler, A&R, Radio Producer, Event organizer, Entrepreneur, Travel & Art Aficionado, Awarded DJ ★
He is the driving force behind the top-selling CD series La Suite, Sunset & Sunrise, Remix by Emporio Armani and, in a recent quote by world renowned DJ Jose Padilla, was mentioned as one of the best CD compilers internationally. His list of past and most recent appearances include sold out events organized by some of the world's top brand names in the music, fashion, hotel and entertainment industries: Tom Ford, Prada, Vogue, Louis Vuitton, MTV, Giorgio Armani, Nobu, Fashion TV, Conde Nast Traveler, Dior, Cartier, Van Clef & Arpels, Pink Elephant - New York, Anantara Resort - Maldives, Matsuhisa Athens, Ku-De-Ta - Bali, 83rd Oscar Awards Event & more…
Chris Child...Ένα όνομα που θα μπορούσε να ονομαστεί ο πιο αντιπροσωπευτικός dj της γενιάς του,με συμμετοχή στα πιο hot και massive house parties της τελευταίας 5ετίας.Γεννημένος το 1987 στην Αθήνα και με μουσικές γνώσεις σε πιάνο και drums,δεν άργησε η αγάπη και η κλίση του για την house μουσική.Το 2010 και μετά απο αρκετά residencies σε γνωστά μαγαζιά της Αθήνας(BABAE,GALEA,BOUTIQUE,BALUX),εντάχθηκε στο δυναμικό των SHOW ME LOVE PARTIES του JOHNNY GERONTAKI(JG). Έχοντας 2 επιτυχημένες σεζόν στο μεγαλύτερο EDM-HOUSE PARTY της χώρας,η ανάγκη του για μια πιο underground μουσική κατεύθυνση,τον βρίσκει στα τέλη του 2011 με την δημιουργία ενός νέου πάρτυ με την ονομασία ΤΗΕ ROLLING STONE.
Rhythmetic CEO, CJ Jeff was brought to the attention of the world dance music circuit when he launched his career in 2001. Born & bred a Greek, whose family thrived in a circle of sociologists, Jeff coined a sound that quickly became distinct and recognizable, with a chic aesthetic to match.
13 years down the line Jeff continues his reign from his humble abode in Athens's. With a highly esteemed local residency lasting for over 10 years and an equally impressive 11 years at Mykonos's "Cavo Paradiso" there's no mistaking CJ is a formidable asset to his countries electronic outfit. But his sound has travelled much further afield, having made an impressive appearance at the 2011 Olympic's Closing Ceremony in Athens, and worldwide performances at the likes of Watergate, Berlin & D. Edge, Sao Paulo, Jeff has covered a huge set of ambitions of which he set out to do at day one.
Da Mike is one of the most talented, up and coming house music djs in Greece.
He joined the Global Fantasy team in 2012 and has already had the opportunity to perform with some of the biggest house djs and producers in the world. He has appeared alongside house music trailblazers such as Black Coffee, Osunlade, Manoo, Zepherin Saint, Culoe De Song, Djeff Afrozila, Franck Roger, Boddhi Satva, Jose Marquez and of course his 'partner in crime' Dj Angelo. He has already had numerous performances under his belt in some of the premier venues throughout Athens and Greece. Stay tuned for his first record release as a producer! The future is here!
PLAYMEN is a five year old project by Pavlos Manolis and
Lefteris Xenakis focused on producing dance - pop music and performing DJ sets. Led by their passion for house music and their eagerness to experiment with new styles and tunes, they evolved into one of the hottest duos in the Greek music scene, creating a promising Brand Name. Playmen's original productions have topped the charts and held the "power play" title in most Greek dance-mainstream radios. The production of music that can supreme in the Global industry and electronic dance music (EDM) is their lead concern, since they both have "studied" for years the international scenery, played and experimented in unique house sounds. The need to keep their sets innovating led Playmen into producing unique bootlegs and mash-ups. This is considered to be one of their best figure and have already gained the support of Tiesto's Club Life with the "Supermode vs Hurts – Tell me why life is wonderful Playmen Bootleg" (Tiesto's Club Life 186).
Music aficionado, dj, producer, music in general enthousiast, Nikolaos Pappas aka Junior Pappa hails out of Thessaloniki, Greece.
When he is not performing, he spends time deep inside his personal studio putting into shape & musical forms all those little sparks of inspiration, creating his own music.
Self-taught, aided & driven only by his unconditional love for music and an upbringing in a very musically-influenced home.
Having spent many years playing at all the major local bars & clubs of his city & later the entire country, holding multiple residencies, Djing special events, promoting his own series of parties & events, Junior Pappa has had a lot of time to develop his own sound.
For him, it's all about the feeling, the tremendous bond he creates and feeds from, with the crowd that follows him wherever he performs. The intensity & passion he puts throughout his performances and production skills, are what characterizes him amongst his peers.
Appearances & performances all around Greece and abroad, releases on domestic and foreign respected labels with more to come in the near future, although Junior Pappa holds a 19 year experience, he feels he has just began and continues to learn everyday something new about his craft and electronic music.
At the end, his main purpose, is to play the sounds that he loves and make people dance.
Junior Rush's debut in the music scene came rather unexpectedly in 1997, when at the age of 17 he became resident Dj in the biggest and hottest dance club of Greece at the time. Amfiteatro! It was there that he had the chance to develop his sets playing for 5.000 people every night and work with great names of the international music dance scene for the first time.
Junior Rush continued a residency at Kingsize club. It is there that he became well known as he had the privilege to open for every guest.
Traveling the world and playing at some of the most prestigious clubs and events, DJ and Producer Vassili Tsili Christos has become recognised for his musical talent. Vassili's unique style offers a wide-ranging mix of House Music and he is known never to disappoint a crowd. His passion for music and his extensive experience as a DJ are the main reasons behind his success and international recognition as well as his impressive.
Hailing from the Greek capital, Thodoris Triantafillou has excelled in all corners of the industry after devoting his life and career to a creative output that illustrates a concoction of influences from across the musical sphere. Recognized by peers and fans through a stream of compelling releases on labels such as Rhythmetic and Souvenir, it's the composition of the philosophy behind a record, the raw energy that electronic formats have to offer, and an exploration of the inner self that makes Thodoris's productions stand out amongst the rest.
Over the past 12 months, Thodoris' position within the dance music industry has propelled after breaching the national Greek iTunes Top 10 with "The Way", co-produced with regular associate, CJ Jeff.
Born in Greece. Maria has realized her love about music during her studies.
She is working as a TV Presenter on MAD TV, presenting every Friday and Saturday the "Dance Zone" music show and as radio producer at MAD Radio 106.2 in Athens.
(Monday-Friday 13.00-17.00 "People's Choice")
In 2011 she has successfully completed the "dj school" and since then she is performing as Guest Dj across the country but also abroad in Lebanon, Egypt, Germany, Spain, Italy, Cyprus and more always at the best bars and clubs.
Page Visitors


text:
Using the now classic metaphor, if we imagine all of human evolution to have occurred in the past hour, the last 550 years that the World Simulation attempts to simulate is no more than a few tenths of a second. While these final tenths have brought us tremendous technological advances, they have also brought us unparalleled global inequality, the most deadly wars of all time, and a precarious environmental situation. Our population is more than 10 times what it was just a few short tenths of a second ago. The richest 225 humans on earth have more wealth than the poorest 2.5 billion people combined and the richest 20% of humans on earth account for 86% of consumption and on average make over $25,000/year. Meanwhile, 1.2 billion people make less than $1/day and over half the world makes less than $2/day. Humans produce more than enough food to feed everyone in the world, but at least 800 million people are starving. In 2004, worldwide military expenditures were $950 billion. In that same year, Worldwatch estimated that it would cost just $12 billion for reproductive health care for all women, $19 billion for the elimination of hunger and malnutrition, $10 billion for clean drinking water for all, and $13 billion to immunize every child in the world from common major diseases. In these final few tenths of a second we have created a global economy running on nonrenewable fossil fuels, all of which will be gone within the next second on our imaginary clock. The use of these fuels has increased carbon dioxide levels by almost 30%, nitrous oxide by about 15%, and concentrations of methane have more than doubled – all of which contribute to a rise in global temperature leading to rising sea levels, expanding deserts, and more intense storms. Perhaps most dramatic, it is in these final tenths of a second on our metaphorical clock that we human beings have attained the ability to literally stop the clock and annihilate ourselves. Whether or not the clock keeps ticking into the next hour will largely be up to the students we are now teaching. This is no small task they face. It may take an almost complete reinvention of how we live and a total revision of how we see the world and our fellow human beings.
So how do our students view these problems and what do they plan on doing about them? Some students are well aware of these issues and are seriously engaged in finding solutions. Unfortunately, the more common perception among students is that these problems are not theirs to solve. Technology will take care of the environmental problems and those in poverty should take care of themselves. "We" are rich because we are smart, hard-working, and have our head on straight. "They" are poor because they are lazy, not smart, and probably corrupt. In short, our system works. Their systems do not. There is little recognition that "our system" might in some ways depend on those of others and vice versa – that perhaps there is ultimately only one system after all, the world system.
It is almost impossible to say all that and keep the attention of those who don't want to hear it. These are statements that are destined to always be preached to the choir and not far beyond. Fortunately these statements are really secondary to what we really need from our students: good questions that will drive them to understand more about our world and become active and responsible global citizens working to ensure our clock keeps ticking.
The World Simulation is designed to point the way to the questions, while only tentatively suggesting a few answers. It attempts to bring the entire world into one single room to give students a brief glimpse into how the world works, what the problems are, and how the conditions of one human group are profoundly dependent on the conditions of other human groups, even if those groups may be separated by vast oceans.
In this post I will lay out the basic rules of the simulation. Unfortunately I cannot reveal too much because the simulation relies heavily on an element of surprise and some students may find their way to read this.
The Basics
The simulation is made up of 4 or 5 interaction rounds. Each interaction round runs for about 12 minutes followed by a 3 minute intermission allowing students to take account of what has occurred and to see if they can "feed themselves." The main rule of the simulation is very simple: in order to survive, at the end of each round each student must have a piece of food (cereal) to eat. This will require either land (represented by a cereal box from which the student can get food) or money to buy food from others. If a student cannot eat at the end of the interaction, their death is marked as a "famine" by the "cultural historian" (see below) and the death decreases the total population of the culture by 5%. The student who starved must go to a nearby land as a refugee and hope for the best.
After the intermission, there is a 3 minute "news update" which I use to draw connections to real world events that are currently being simulated. The news is an audio-visual extravaganza complete with commercials advertising some of the new products being created by emerging imperialists.
At the beginning of the simulation, each group has at least 3 things: a box of cereal representing their "land," envelopes to be opened at the beginning of each round (providing various challenges or instructions), and a collection of colored notecards representing various resources or goods that they can trade with others (these can represent many things depending on their ethnography, but some examples are white = salt, green = plant materials, orange = obsidian, and pink = shells).
All the props cost me just over $100. The most important props are boxes of cereal. There are three types of cereal in the simulation, each one of them profoundly symbolic. Fruit Loops represent a rich, varied, and nutritious diet. I'm aware of the irony of this, but the multiple colors are what set them apart. Cocoa Puffs represent luxury consumption goods such as cocoa, coffee, sugar, and tobacco. Cheerios represent large-scale monocrop cultivation. Of course, almost as soon as the simulation begins all of the cereals begin to take on different meanings for different people, which is exactly how it should be.
Each culture also has one sacred item, usually represented by a stuffed animal. The meanings they attach to this are up to them.
Populations are adjusted to represent the estimated population of the world in 1450 CE (about 400 million). The population automatically grows in each interaction, simulating the real world's population growth so that we end the 2nd to last round with 6.2 billion people. (The last round is a special "future projection" round in which we try to solve all of the problems we have created.) Throughout the simulation the population of each group may increase or decrease based on famine, disease, or a shift to a new subsistence pattern (e.g. industrial agriculture would increase the "carrying capacity" and thereby increase the population).
Based on the ethnographies, I create mobility maps for each group showing where they are able to travel. Anybody who travels must carry a mobility map with them and cannot go beyond the boundaries that their mobility map sets for them.
I select 3-5 groups who seem to be on the verge of seeking other areas to explore or colonize. They are given full mobility to travel the entire world and a separate set of instructions with a box of materials that facilitate the colonization of other lands. Their materials include colored flags to mark their conquests, various tools and materials to facilitate production of luxury goods, money, and a special map that tells them where certain luxury goods will grow and where they will not (so Cocoa Puffs only grow in certain areas, Fruit Loops grow better in some areas than others, etc.). I can't reveal those materials or instructions here because much of the simulation depends on an element of surprise that forces students to find solutions to emerging issues or capitalize on the opportunities given. Starting in the second round, these three groups are also required to find fossil fuels (represented by yellow notecards) to power their industrial revolution. They need to turn in one yellow card at the end of each round or they lose half of their hard power. There are not enough yellow cards in the room to last until the end of the last round (representing 100 years into the future).
Those with money can participate in the world market exchange – a table in the front of the room where groups can exchange money, natural resources and hard power. Exchange rates change throughout the simulation to simulate technological developments, scarcity, and other factors.
Hard Power & the Rules of War
Each culture starts with a certain amount of hard power with which to launch attacks or protect themselves. Hard power is represented on small cards with numbers ranging from 0 to 1,000,000. Each culture has as many as 50 hard power cards with different numbers on them. The total amount of hard power a culture starts with depends on their population, technology, and other relevant cultural factors. When traveling a student should carry some of this hard power with them either for protection or conquest, however they have to strategize because taking too much depletes the amount of hard power others in the group can carry or could leave the homeland completely unprotected. Alliances can also be made, allowing one culture to draw on the hard power of the other and vice versa.
A battle begins when somebody from one culture challenges another. Both sides quickly decide how much hard power they want to use in the first battle and place these cards in their right hand. At the count of 3 each side shows the other the hard power they are holding in their right hand. The side with the most hard power wins the battle and gets all of the hard power expended by the other culture. The war is over when one side surrenders or is completely out of hard power. Terms of surrender are negotiable between the two warring parties but may include a right to hold on to some hard power, money, land rights, etc.
The winner occupies the land and then acts as a colonizer or occupier. The colonizer can tax the people, take the land, force people into labor, or force them to grow different crops.
Of course, this is where it gets interesting.
As I said before, I cannot reveal too much, but this is how the simulation has typically gone in the past 3 trials:
We start by arranging the tables in the room to match our world map as closely as possible:
Emerging colonial powers almost immediately begin exploration and colonization and have colonized most of the world by the end of the second round.
Colonizers often attempt to completely transform local economies. They tax or take the land (the box of Fruit Loops), forcing the local people to work for money to survive. This not only creates a cheap labor pool, it also creates a market for their exports (Cheerios). They use the cheap labor to manufacture luxury goods (Cocoa Puffs and Fruit Loop necklaces).
As the simulation proceeds, the colonies begin to acquire an emerging sense of nationalism and a growing population. Eventually they overthrow their colonizer, or the colonizer finds it too difficult to manage the colony. There are often several atrocities during this phase of the simulation that send refugees to other lands. Several new nation-states also form during this time.
Even as processes of decolonization set people "free," they still find that they are dependent on a global system of trade in which they are working for low wages and spending all of their money on goods exported from previous colonial centers. The world system of the real world maps onto our simulated world:
Prior to the final round, students are asked to look around at the world they have created. We take a quick survey to determine how our numbers compare to those of the real world. Like the real world, over half of the people in our world are working for very little money and struggle to find enough food to eat. I announce that there are not enough yellow cards to power the fossil fuel economy through the next round. When they open their envelopes for the final round, they find more problems.
The last round often inspires valiant efforts to solve the world's problems. Some students try to create expansive alliances with other low-wage laborers to drive wages hire. Others stage protests and beg for empathy from the colonizers.
In our simulation, they have only 10 minutes to think through solutions – a trivial amount of time. The solutions we create are likewise trivial. But the questions the simulation inspires are not. And if students leave the simulation asking questions they didn't ask before the simulation, or begin asking questions in different ways, there is no telling what might happen. Maybe it will inspire them to keep that clock ticking afterall.

subset: commoncrawl


text:
This plugin has been closed as of জুলাই 8, 2019 and is not available for download. Reason: Guideline Violation.
This plugin provides 1 block.
- photo-gallery-image/index
- GrandWP Gallery
জুন 26, 2019
Very well done indeed, but... The first feature I miss is that it does not show an overview of all photos and videos combined from all already created galleries. The second feature I miss even more is the ability to simply duplicate an existing gallery that can then quickly be modified. Instead, you have to start all over again very time you create a new gallery, even when you only want to change a few things from an already existing one that you want to keep as is. Very time consuming! With those features the plugin would deserve 10 stars (even when 5 is the max they allow you to give it!).
জুন 4, 2019
It is good gallery for view photo easily i am using this and it is great application and in application you get more features than other appliction. Awesome look of application..
জুন 3, 2019
Every person should use this responsive photo gallery
মে' 27, 2019
Really simple to use and very good functionalities also in the free release
অক্টোবৰ 19, 2018
Very fast for basic setup, a bit harder for the more advanced settings.
জুলাই 25, 2018
Really like this plugin for its responsiveness and easy interface. Also, they have provided fast support and where so kind to explain all possible ways to have the results I wanted.
Contributors & Developers
"Responsive Photo Gallery – Images Gallery for WordPress" is open source software. The following people have contributed to this plugin.Contributors
Translate "Responsive Photo Gallery – Images Gallery for WordPress" into your language.
Interested in development?
Browse the code, check out the SVN repository, or subscribe to the development log by RSS.


text:
Candida dubliniensis is an opportunistic yeast closely related to Candida albicans that has been recently implicated in oropharyngeal candidiasis in human immunodeficiency virus-infected patients. Most manifestations of candidiasis are associated with biofilm formation, with cells in biofilms displaying properties dramatically different from free-living cells grown under normal laboratory conditions. Here, we report on the development of in vitro models of C. dubliniensis biofilms on the surfaces of biomaterials (polystyrene and acrylic) and on the characteristics associated with biofilm formation by this newly described species. Time course analysis using a formazan salt reduction assay to monitor metabolic activities of cells within the biofilm, together with microscopy studies, revealed that biofilm formation by C. dubliniensis occurred after initial focal adherence, followed by growth, proliferation, and maturation over 24 to 48 h. Serum and saliva preconditioning films enhanced the initial attachment ofC. dubliniensis and subsequent biofilm formation. Scanning electron microscopy and confocal scanning laser microscopy were used to further characterize C. dubliniensis biofilms. MatureC. dubliniensis biofilms consisted of a dense network of yeasts cells and hyphal elements embedded within exopolymeric material.C. dubliniensis biofilms displayed spatial heterogeneity and an architecture showing microcolonies with ramifying water channels. Antifungal susceptibility testing demonstrated the increased resistance of sessile C. dubliniensis cells, including the type strain and eight different clinical isolates, against fluconazole and amphotericin B compared to their planktonic counterparts. C. dubliniensis biofilm formation may allow this species to maintain its ecological niche as a commensal and during infection with important clinical repercussions.
Candida dubliniensis is a newly described and widely distributed yeast species that is closely related to Candida albicans (12, 14, 20, 30, 34, 36, 37, 47, 55, 59-62). Indeed, C. dubliniensis was initially difficult to distinguish from C. albicans and was often misidentified as such in standard clinical laboratory tests because of phenotypic and genotypic characteristics closely shared with the latter (6, 12, 17, 21, 29, 34, 36, 47, 51, 55, 58, 60). Most C. dubliniensis clinical isolates are susceptible to existing antifungal agents, although the inducibility of fluconazole resistance in vitro has been described (34, 38, 39, 50, 52, 54). Thus, the frequent use of fluconazole prophylaxis may contribute to the emerging role of C. dubliniensis as a pathogen.
C. dubliniensis is a causative agent of oropharyngeal candidiasis (OPC) in human immunodeficiency virus (HIV)-infected and AIDS patients (12-14, 29, 30, 34, 36, 37, 56, 61, 62). Other forms of mucosal candidiasis are frequently encountered in other patient groups, such as denture wearers, cancer patients, infants, and the elderly (11). In order to colonize and infect the oral environment, yeast cells must first adhere to host cells and tissues or prosthetic materials within the oral cavity or must coaggregate with the oral microbiota (7, 8, 10, 20, 27, 28, 31, 42, 53, 57). In the case of C. dubliniensis these adhesive properties may be facilitated by the intrinsic cell surface hydrophobicity displayed by this organism (26). Initial attachment of cells is followed by proliferation and biofilm formation, with sessile cells in biofilms displaying properties that are dramatically different from their planktonic (free-living) counterparts. Two consequences of biofilm growth with profound clinical implications are the markedly enhanced resistance to antimicrobial agents and protection from host defenses (9, 15, 16, 18, 19, 48, 64). While previous studies of biofilm development and species interaction have focused largely on bacterial species, relatively little is known about fungal biofilms. C. albicans biofilms share several properties with bacterial biofilms, including their structural heterogeneity, the presence of expolymeric material, and their decreased susceptibility to antimicrobial agents and biocides (1-5, 23, 24, 27, 32, 65). However, the ability ofC. dubliniensis to form biofilms has not been evaluated. We have previously shown that C. dubliniensis was able to withstand competitive pressure from C. albicans when both species were grown under biofilm-inducing conditions but not when they were grown as planktonic cultures (33). Understanding the biofilm lifestyle is, therefore, critical in relation to therapeutic strategies engaged to treat OPC and related infections, as it is more than likely that microbial biofilms will be the predominant mode of growth. Here, we describe models and characteristics of C. dubliniensis biofilms.
Organisms.Nine isolates of C. dublinenisis were used in the course of this study: eight clinical strains (1154, 1231, 1439, 2419, 3698, 4516, 4572, and 4712), isolated from oropharyngeal samples taken from HIV-infected patients, as previously described by Kirkpatrick et al. (1998), and C. dubliniensis type strain NCPF 3949. All strains were stored on Sabouraud dextrose slopes (BBL, Cockeysville, Md.) at −70°C.
All C. dubliniensis isolates were propagated in yeast-peptone-dextrose (YPD) medium (1% wt/vol yeast extract, 2% wt/vol peptone, 2% wt/vol dextrose [US Biological, Swampscott, Mass.]). Flasks containing liquid medium (20 ml) were inoculated from YPD agar plates containing freshly grown C. dubliniensis and incubated overnight in an orbital shaker at 30°C until the cells had reached a stationary phase of growth. All strains grew in the budding-yeast phase under these conditions. Cells were harvested and washed in sterile phosphate buffered saline (PBS) (10 mM phosphate buffer, 2.7 mM potassium chloride, 137 mM sodium chloride, pH 7.4 [Sigma, St. Louis, Mo.]). Cells were suspended in RPMI-1640 supplemented with l-glutamine, buffered with morpholinepropanesulfonic acid (Angus Buffers and Chemicals, Niagara Falls, N.Y.), and adjusted to the desired cellular density by counting in a hematocytometer (see below).
Biofilm formation on the surface of wells of microtiter plates. C. dubliniensis biofilms were formed on commercially available presterilized, polystyrene, flat-bottomed, 96-well microtiter plates (Corning Incorporated, Corning, N.Y.). Biofilms were formed by pipetting standardized cell suspensions (100 μl of a suspension containing 5.0 × 106 cells/ml in RPMI-1640) into selected wells of microtiter plates and incubating over a series of time intervals (0.5, 1, 2, 4, 6, 8, 24, and 48 h) at 37°C. After biofilm formation, the medium was aspirated and nonadherent cells were removed by thoroughly washing the biofilms three times in sterile PBS. A semiquantitative measure of biofilm formation was calculated using a 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) reduction assay, which measures metabolic activity of cells, essentially as described before (22, 25, 63). Briefly, XTT (Sigma, St. Louis, Mo.) was prepared as a saturated solution at a concentration of 0.5 g/liter in Ringer's lactate. This solution was filter sterilized through a 0.22-μm-pore-size filter, aliquoted, and stored at −70°C. Prior to each assay, an aliquot of stock XTT was thawed, and menadione (Sigma) added to a final concentration of 1 μM. A 100-μl aliquot of XTT-menadione was then added to each prewashed biofilm and to control wells (to measure background XTT levels). The plates were then incubated in the dark for 1 h at 37°C, and the colorimetric change (a direct reflection of the metabolic activity of the cells within the biofilms) was measured in a microtiter plate reader at 490 nm (Benchmark Microplate Reader; Bio-Rad, Hercules, Calif.). Microscopic examinations of biofilms formed in microtiter plates were performed with light microscopy using an inverted microscope.
Preconditioning films with serum and saliva.Serum was collected from patients at the University Hospital, University of Texas Health Science Center at San Antonio, who tested negatively for both HIV and hepatitis B infection. The serum was pooled, aliquoted, and stored at −70°C. Saliva was collected from several volunteers, who chewed parafilm to stimulate their salivary glands for 1 h, and it was collected in 50-ml Falcon tubes on ice and then centrifuged at 3,000 × g . The saliva was pooled, aliquoted, and stored at −70°C. Serum and saliva were prepared at 50% (vol/vol) in sterile PBS, individually dispensed (50 μl) into six wells of a microtiter plate, and then incubated in the wells overnight at 4°C. Excess serum and saliva were aspirated, and the adsorbed conditioning film was washed once in sterile PBS. C. dubliniensisisolates were washed in PBS, resuspended at a concentration of 108 cells per milliliter in RPMI-1640, and dispensed (100 μl per well) into the 96-well microtiter plates and incubated for 30 min, 4 h, and 24 h at 37°C. For each time point the wells were aspirated and washed three times in sterile PBS, and adhesion was measured by the XTT reduction assay and by light microscopy.
Experiments were performed twice with six replicates for each condition. Results were analyzed by using an unpaired and two-tailed Student t test.
SEM.For scanning electron microscopy (SEM), biofilms were formed on polymethylmethacrylate (PMMA) disks (diameter, 25 mm), which were prepared by combining Dentsply repair material-lucitone Fas-PorE pourable denture base liquid with Dentsply repair material powder (Dentsply International Inc., York, Pa.) at a ratio of 7.5 ml of monomer liquid to 10 g of powder, and mixed thoroughly. The PMMA solution was then quickly poured into Teflon molds and allowed to polymerize into rigid disks. The disks were then washed in sterile distilled H2O to remove toxic monomer residues and sterilized with 70% (vol/vol) alcohol. Biofilms were formed on PMMA discs within six-well cell culture plates (Corning International, Corning, N.Y.) by dispensing standardized cell suspensions (4 ml of a suspension containing 5.0 × 106 cells/ml in RPMI-1640) onto appropriate disks at 37°C. The disks were removed at selected time intervals (2, 4, 6, 8, 24, and 48 h) and washed as described above. The biofilms were then either air dried or placed in a fixative (4% formaldehyde [vol/vol], 1% glutaraldehyde [vol/vol] PBS) overnight. The samples were rinsed in 0.1 M phosphate buffer (2 times, 3 min each) and then placed in 1% Zetterquist's osmium for 30 min. The samples were subsequently dehydrated in a series of ethanol washes (70% for 10 min, 95% for 10 min, and 100% for 20 min), treated (2 times, 5 min each) with hexamethyldisilizane (Polysciences Inc., Warrington, Pa.), and finally air dried in a desiccator. The specimens were coated with gold-palladium (40%/60%). After processing, samples were observed with a scanning electron microscope (Leo 435 VP) in high vacuum mode at 15 kV. The images were processed for display using Photoshop software (Adobe Systems Inc., Mountain View, Calif.).
Confocal Scanning Laser Microscopy (CSLM).Biofilms were formed as described above for SEM but using 15-mm-diameter plastic coverslips. After incubation at 37°C for different periods of time, they were washed with PBS and stained using the FUN 1 fluorescent cell stain (Molecular Probes, Eugene, Oreg.). This product was used here as a nonspecific stain for all cells within the biofilm, with no differentiation between metabolically active and inactive cells. Stained biofilms were observed with an Olympus FV-500 laser scanning confocal microscope, using a 488-nm argon ion laser. Serial sections in the xy plane were obtained at 1-μm intervals along thez axis. Three-dimensional reconstructions of imaged biofilms were obtained by the resident software. The images were processed for display using the Adobe Photoshop program (Adobe).
Antifungal susceptibility testing.Two clinically used antifungal agents were used in this study, fluconazole (Pfizer, Inc., New York, N.Y.) and amphotericin B (Bristol-Myers Squibb, Princeton, N.J.). Fluconazole and amphotericin B were prepared at stock concentrations of 1,024 μg/ml in RPMI-1640 (Angus Buffers and Chemicals) and antibiotic medium 3 (Difco Laboratories, Detroit, Mich.), respectively. Antifungal susceptibility testing to determine MICs for planktonic cells was performed by using the National Committee for Clinical Laboratory Standards (NCCLS) M-27A broth microdilution method with reading of endpoints at 48 h (41). For antifungal susceptibility testing of sessile (biofilm) cells, biofilms were formed by pipetting standardized cell suspensions (100 μl) into selected wells of the microtiter plate, as described above, which were then incubated for 48 h at 37°C. The biofilms were then washed thoroughly three times with sterile PBS before the addition of antifungal agents in serially double-diluted concentrations and incubated for a further 48 h at 37°C. A series of antifungal free wells was also included to serve as controls. MICs for sessile cells were determined at 50% inhibition (SMIC50) and at 80% inhibition (SMIC80) compared to results with drug-free control wells using the XTT reduction assay described above.
In vitro biofilm formation by C. dubliniensis.The kinetics of biofilm formation by the C. dubliniensis type strain on the surface of polystyrene wells over 48 h as revealed by the colorimetric XTT formazan salt reduction assay is illustrated in Fig. 1. The metabolic activity of cells in the biofilm increased over time as the cellular mass increased. The biofilms were highly metabolically active in the first 8 h. However, as the biofilm matured and the complexity increased (24 to 48 h), the metabolic activity reached a plateau but remained high, which reflected the increased number of cells that constituted the mature biofilm. Experiments were performed in sets of eight replicates on three separate occasions, with similar results obtained in all experiments.
Light microscopy was used in conjunction with this semiquantitative method to monitor biofilm formation and morphological characteristics of adhered cells. At 30 min, adherent budding yeast cells were observed sticking to the wells in a random manner (the origins of microcolonies). Germ tube formation occurred within 2 h, and the presence of filamentous forms was noticeable at 4 and 6 h. After 8 h of adhesion a monolayer of yeast cells, germ tubes, hyphae, and pseudohyphae was observed covering the entire surface of the well. As the biofilm matured after 24 and 48 h of growth, the complexity of the biofilm increased into a multilayered biofilm matrix, with all fungal morphologies being present in the final biofilm structure.
The kinetics of biofilm formation by the different clinical isolates showed a pattern similar to that of the type strain. However, in relation to their metabolic activities and individual propensity to adhere to the polystyrene wells, strain variations were detected (results not shown).
Effect of serum and saliva conditioning films on C. dubliniensis adhesion and biofilm formation.The effect of serum and saliva conditioning films on C. dubliniensisadherence and biofilm formation is shown in Fig.2. When serum was provided as a conditioning film, the level of C. dublinienisis adherence was significantly elevated in comparison to that observed in untreated wells. The effect was most clearly demonstrated during early adherence at 30 min (P < 0.0001) . After 4 and 24 h a smaller difference was observed between biofilm formation in the presence and absence of the serum pellicle, but differences were still statistically significant ( P = 0.0003 and 0.0001, respectively). This pattern of adherence was observed consistently for two other strains examined (data not shown).
When saliva was provided as a conditioning film, the level of adherence of C. dubliniensis was elevated in comparison to results with polystyrene. In contrast to the case with serum, the effect was marginally increased at 30 min (P = 0.0185) . However, after 4 and 24 h there was a larger increase in biofilm metabolic activity ( P = 0.0002 and < 0.0001 , respectively), comparable to that observed in the presence of serum pellicle. The effect of saliva conditioning films on adherence was generally lower than that of serum; however, a general trend in increased adherence and biofilm formation was equally observed. Overall the results showed that biological conditioning films help provide receptor binding sites for planktonic C. dublinienis.
SEM and CSLM visualization of C. dubliniensisbiofilms.Biofilm formation by C. dubliniensis on acrylic discs was monitored by SEM (Fig.3 and 4). Despite its destructive nature, SEM observations provided useful information on the different cellular morphologies present in the biofilm structure. Initial adherence of yeast cells was followed by germ tube formation and subsequent development of hyphae (Fig. 3). Mature biofilms consisted of a dense network of cells of all morphologies, deeply embedded in a matrix consisting of expolymeric material, which was better preserved when the biofilm samples were air dried (Fig. 3).
The noninvasive CSLM technique enabled imaging of intact biofilms and visualization of the three-dimensional distribution of labeled C. dubliniensis cells in the context of the complex biofilm community. Significant channeling and porosity were also observed. Overall, results indicated that mature C. dubliniensisbiofilms displayed a typical microcolony/water channel architecture with extensive spatial heterogeneity. Figure5 shows a three-dimensional reconstruction of a 24-h-old, 40-μm-thick C. dubliniensisbiofilm resulting from the compilation of a series of individualxy sections taken across the z axis.
Susceptibility testing of C. dubliniensis biofilms against clinically used antifungal agents.The in vitro activity of clinically used fluconazole and amphotericin B against preformedC. dubliniensis biofilms was assessed using the modified XTT assay. Experiments revealed the increased resistance of sessileC. dubliniensis cells. The results, expressed as SMIC50s and SMIC80s, are presented in Table1. Data revealed that C. dubliniensis biofilms were intrinsically resistant to fluconazole. Amphotericin B demonstrated certain activity against C. dubliniensis biofilms, as indicated by SMIC50s. However SMIC80s were substantially increased (up to 64-fold higher than the corresponding MICs) and already fell within the resistant range for this antifungal agent (>1 μg/ml).
We have demonstrated the ability of C. dubliniensis to adhere to and form biofilms on the surfaces of different biomaterials. A semiquantitative colorimetric method based on XTT reduction allowed monitoring of the different stages in biofilm formation on the wells of microtiter plates. The presence of serum or salivary pellicles, which are normally found in the oral environment and may provide binding sites for Candida (8, 10), increased the initial adherence of C. dubliniensis cells to biomaterials and subsequent biofilm formation. Other investigators have previously shown that the presence of serum and salivary pellicles can potentiateC. albicans colonization of acrylic strips and denture lining materials (41-46).
SEM techniques revealed that similar to C. albicans biofilms (5, 23), mature C. dubliniensis biofilms consist of a mixture of yeast and filamentous forms embedded within exopolymeric material. The exopolymeric material was better preserved when biofilm preparations were air dried, whereas in fixed biofilms only its remains were visible attached to the surfaces of the cells (Fig. 3 and 4). The nondestructive CSLM technique (Fig. 5) allowed in situ visualization of hydrated biofilms and demonstrated that C. dubliniensis biofilms possess structural heterogeneity and display a typical microcolony/water channel architecture similar to what has been described for bacterial biofilms (49, 64). This structure may represent an optimal arrangement for the influx of nutrients, disposal of waste products, and establishment of microniches throughout the biofilm (15, 16).
Currently there is a method well described by the NCCLS (M-27A) for determining antifungal susceptibilities for yeast planktonic cultures (40). Nevertheless, the susceptibility data generated from this approach do not account for the intrinsic resistance exhibited by sessile cells. For example, Hawser and Douglas (24) reported that a range of antifungal agents were between 30 and 2,000 times less active against C. albicans biofilms than against planktonic cultures as measured by MICs. In agreement with these observations, here we have demonstrated the intrinsic resistance ofC. dubliniensis biofilms to fluconazole, the most commonly used antifungal agent for the treatment of OPC, and their increased resistance to clinically used amphotericin B. Although amphotericin B exhibited a certain degree of activity against biofilms as indicated by SMIC50s, the SMIC80s, all above 1 μg/ml, already fell into the range indicating resistance according to interpretative break points (41). Although these concentrations represent obtainable drug levels, the intrinsic toxicity displayed by this agent will preclude its use at such a high dose. Moreover, even at higher concentrations (up to 16 μg/ml) sterility was never achieved. Factors that may be responsible for the increased resistance of microbial biofilms include restricted penetration of antimicrobials due to the exopolymeric material, a decreased growth rate (physiological status) of cells within the biofilm, and differential gene expression. An interesting possibility to explain biofilm resistance has been proposed recently by Lewis, that is that the majority of cells within the biofilm are not necessarily more resistant to killing than planktonic cells, but rather a few persisters survive and are actually preserved by antibiotic pressure (35). Overall, the disparity between MICs for planktonic and sessile cultures from an identical isolate may, therefore, explain why antifungal treatment may be ineffective in some instances and may partially explain the lack of an absolute correlation between clinical (in vivo) and mycological (in vitro) resistance.
The ability of C. dubliniensis to form biofilms may confer on this microorganism an ecological advantage in trying to maintain its niche as a commensal and pathogen of humans by evading host immune mechanisms, resisting antifungal treatment, and better withstanding the competitive pressure from other oral microorganisms. Fungal biofilms may also serve as a safe reservoir for the release of infecting cells into the oral environment. Thus, biofilm formation in C. dubliniensis may represent a key factor for the survival of this species, which seems to be particularly well adapted to colonization of the oral cavity, with important clinical repercussions.
This work was supported by grant ATP 3659-0080 from the Texas Higher Education Coordinating Board (Advance Technology Program, Biomedicine). J.L.L.-R. is the recipient of a New Investigator Award in Molecular Pathogenic Mycology from the Burroughs Wellcome Fund.
We thank T. F. Patterson for C. dubliniensis clinical isolates. We thank Peggy Miller and Victoria Frohlich (Depts of Pathology and Cellular and Structural Biology) for assistance in SEM and CSLM experiments, respectively.
- Received 23 March 2001.
- Accepted 14 June 2001.
- Copyright © 2001 American Society for Microbiology


text:
By Sarah Christmyer
Rushing to the car with a cart full of groceries, I hit a pothole. Not a big one, just big and deep enough to grab a front wheel. The cart flipped forward, and I flipped with it. It all happened in slo-mo: the bar hitting my shin, me recoiling in pain, the coffee flying, me pulling back, the sideways lurch, the catapult, the unforgiving pavement. I lay there stunned, watching people walk into the store, eyes on their cell phones, oblivious or determined not to see.
Life hits like that sometimes. One year, we moved across the country and couldn't sell our house. Not long after, my husband was laid off, we lost our savings, my grandpa died, and my mom got cancer. "It never rains but it pours," they say, but that doesn't capture the pummeling we feel when bad things won't stop coming and there's no help in sight.
I think of the disciples in the storm in Matthew's gospel. Wave after wave came across the bow of their fishing boat until it seemed they would sink, and Jesus slept. They woke him up; I imagine them shaking him, wild-eyed, begging for help. He did, of course, but it's what he says that grabs my attention: "Why are you afraid, O men of little faith?" (Mt 8: 26)
"Why are you afraid," indeed? Why am I afraid, when storms come and rock my boat, when Jesus is beside me? Even if he seems to sleep: why am I afraid? Do I not have faith that he will help? Jesus calmed the sea, but he also is the master of bringing life out of the jaws of death. I wonder if there was some greater good that might have come about, had they had faith to wait.
"Stay in the boat in which he has placed you, and let the storm come. Long live Jesus!; you will not perish. He may sleep, but at the opportune time he will awake to restore your calm." (Saint Pio of Pietrelcina)
About the author:
Sarah Christmyer is co-developer and founding editor of The Great Adventure Catholic Bible study program. The author of numerous Bible studies and several guided journals for Bible reading, she speaks at conferences and retreats on topics related to Scripture and the Catholic faith. She teaches at St. Charles Borromeo Seminary, Philadelphia. Follow her blog at ComeIntotheWord.com.
Adapted with permission from www.ComeIntotheWord.com under the title "When the waves come: Stay in the boat" originally published on 11/14/2014.
Sarah, I needed to read this today. Sometimes we think our lives are so burdened, but God is there, always! Thank you and God bless you! +JMJ+
Thank you for this beautiful message! I have been through some stormy weather and come out on the other side to see the peace and calm.
Thank you for this needed insight. Matthew 26:8 is one of my favorite lines from the New Testament. Life is very bumpy at times and we need to remember we are never alone. Thanks.
Thank you, Sarah, for your message. Another devotion I read today, revealed the same message. Life is messy and complicated; there are times when we may not feel HIS presence. But, our God is available 24/7! When He is not walking beside us, He is carrying us.
For the past several months, I have been working on starting a new Women's ministry in my church. Although I've prayed over such a ministry for the last two years and felt the call, I have had some bumpy delays while trying to be patient with those helping to provide resources that are not available to me otherwise. I am so thankful I have a merciful God, who has gifted me with insight to adjust my sails, to hang on and to persevere.
Growing up and into my young adulthood, I faced trials that I would not wish on anyone. It's those times that remind me that what I am going through right now is so small in comparison. I love the quote you shared from Saint Pio of Pietrelcina, "Stay in the boat in which he has placed you, and let the storm come. Long live Jesus!; you will not perish. He may sleep, but at the opportune time he will awake to restore your calm." .
May we always Submit ourselves to our Lord, Pray without ceasing and Trust in His timing … when we need Him most, He shows up large and in charge! God bless!
Totally needed to hear this today! It's exactly how I feel at this moment in my life. Thank you for sharing!
Thanks for writing, all you who feel "in the same boat" as it were. As I ride the waves, I'll pray for you as well. Blessings, Sarah.
Beautiful post, Sarah! Thank you, sweet friend!


text:
Round Beach Towel - "Voyage Around The World"
Regular price $ 34.99
Unit price per
SwimZip's trendy round beach towels are perfect for a day at the beach or pool. Spread out on this soft cotton circle towel and stop worrying about falling off the edge - there's so much space! Stylish black and white design. The fun and functional "SwimZip Circle Towel"! World print on a towel makes this the perfect towel to relax on the beach.
Our large, 100% velour cotton towel measures 5 feet (1500 mm) in diameter and features a fun tasseled edge. These towels also make great beach blankets. Round beach towel - Yes - The Best Roundie
Customer Reviews
The beach towel is excellent quality! This was the only place I could find that towel at such a decent price.
It is great quality and the round cut seems more suited to the human body. Why not make bath towels?
I love the Round Beach Towel I received "Ocean Blues". When my friend showed me the product and that she was working for them I fell in love and immediately got on line and bought one. The product is quality work and fun. I look forward to many more products.
So cozy! Thick quality and my daughter is using it as a blanket in the winter! Towel in the summer. Great purchase!
This is MY towel! It's the perfect size, absorbent, can wrap it around you or the kids, just a fav all around. The black and white pattern is awesome too! Love it!


text:
richie hawtin's clothing line...
- open
- mnml maxi
- Posts: 1764
- Joined: Thu Jul 26, 2007 9:59 pm
- Location: Belfast N, Ireland
- masterpiece
- mnml maxi
- Posts: 553
- Joined: Thu Oct 05, 2006 9:10 pm
- pantycontrol
- mnml mmbr
- Posts: 180
- Joined: Sat Apr 21, 2007 8:45 pm
- Location: seattle
- Contact:
- pantycontrol
- mnml mmbr
- Posts: 180
- Joined: Sat Apr 21, 2007 8:45 pm
- Location: seattle
- Contact:
for every reason to hate the man there are two reasons to love him.masterpiece wrote:but it makes me more bored to see everyday a richie hawtin topic with haters.....if u hate ; just dont listen , dont open topics .... why do u even care to talk about him...??.yeah im bored ....from stupid ppl...
clothing line?
there are hundreds of artists with clothing lines, etc... if you like the design (which I don't), have a lot of money to throw around and like richie, then I see no difference in this and a playhouse t-shirt.
now let the topic die in peace
cloutier wrote:he comes in around 1994 and leaves around 2002.minimalwyte wrote:When does Digweed come in?
- mnml mmbr
- Posts: 249
- Joined: Fri Nov 17, 2006 12:01 pm
- Contact:
camus wrote:so what? it is very common that labels sells goodies like tshirts , stickers , posters , etc...
furthermore why cant hawtin diversify his activities into fashion? are you jealous ? he is like 40 , maybe he has the right to start making a little money for living ...
how many of you have real jobs along with djing ? so you are evil profiteers because you are an union worker? computer analyst ? teacher or work at the townhall ?
you should stop bullying hawtin , cause you're no better than him... he did more for techno than anyone of you will ever do, but maybe thats why all you can do is spit on him, cause you're not very usefull to the cause otherwise or you believe so.
Stop critisizing him , do your thing , be successfull so that others can spit on you...
LMAO what an asshole
[img][img]http://i167.photobucket.com/albums/u147 ... ri/081.jpg[/img][/img]
------>>>>hey everybody : Im a fcking pedophile faggot
- mnml maxi
- Posts: 771
- Joined: Wed Feb 27, 2008 1:14 am
- Location: New York


text:
The failure of complex projects is prevalent worldwide, namely that the main factors of success – meeting the deadline, remaining within the budget allocation and realising appropriate quality developments – are not be achieved properly, which is frequently caused by low quality project management. Our mission is to contribute to successful investments by our partners by providing them with high quality project management services.
- Strategic consulting connected to the developments of our partners, is an important element of our services. In this context, we actively contribute to the development of project ideas and support the preparation phase of the project.
With our experience and expertise we contribute to the following:
- feasibility study examining the political, economical-financial, legal, technical and environmental circumstances of the project;
- ensuring compliance with corporate level strategic objectives;
- expert preparation of the sponsorship possibilities of the project.
During the preparatory phase we support:
- stakeholder analysis;
- detailed design of the targets;
- preparation of project time scheduling, detailed financial and resource plan.
In addition, we take the main steps of the risk management, and contribute to the formation of the necessary internal organisational arrangements, to the preparation of operating procedures of the project and to the design of the exit strategy.
- financial controlling services; To ensure high quality support of our partners' projects we undertake the operation of a Programme Management Office (PMO).
During its operation, the PMO provides:
- continuous monitoring of implementation of developed plans;
- measuring the fulfilment of quality requirements;
- efficient operation of internal and external communication processes.
In the PMO establishment phase we identify the range of tasks to be performed, the competence of the PMI and the proposed level of organizational embeddedness, considering the organizational and cultural values of the partner enterprises and applied operational mechanisms.
Our important tasks furthermore:
- administrative support of the project managers;
- preparing reports for the senior management;
- development of document templates and methodologies for the project organisation;
- preparation and compliance to the project documentation order.
After the physical implementation of the project, we support with our high level expertise the fulfilment of project closure tasks, the appropriate dissemination of the results, and the coordination and collection of experiences gained during the project.
"Never measure the height of a mountain until you have reached the top. Then you will see how low it was."
Dag Hammarskjold


text:
Home > College of Education > jpme > Vol. 5 > No. 1 (2010)
This special issue of The Journal of Praxis in Multicultural Education celebrates the 20th anniversary of the National Association for Multicultural Education (NAME) and its legacy of educational equity and social justice, by listening to the voices of faculty in the College of Education at the University of Colorado at Colorado Springs (UCCS) as they discuss the latest panoply of research and practices utilized at UCCS and beyond, to prepare educators who are advocates for educational equity and social justice.


text:
A cozy place in Rozenburg
Being one of the oldest catering facilities in Rozenburg, Pico Bar has earned itself a reputation as a music, eating and event cafe.
Our guest profile greatly differs. The weekends are mostly reserved for the youth, while weekdays are reserved for elder guests. Our regular guests form a large and diverse group. In addition to them we also host many international guests. The atmosphere in the café is cozy, friendly and relaxed. Somebody will be open for a chat, a card or dice game, playing darts or snooker almost all the time.
Our kitchen delivers delicious and fresh meals for our guests. Our menu offers different dishes so every taste can find its wish. In addition to the regular menu, we also offer an order menu for both companies and consumers.
In addition to the authentic kitchen, Pico Bar also offers hotel facilities with plenty of rooms. All rooms are decorated contemporary and modern with television, phone, clock radio and internet connection. We offer rooms with and rooms without a shower and toilet.


text:
They call it yoga divorce. It's what allows Ryann and Phil Doucette to remain friends, co-parents and business partners after their split eight years ago. Now they're opening a new plant-based restaurant together called the Seed Café, connected to their studio Modo Yoga at 3252b W. Lake St.
"Relationships fall apart, but you have a choice in how you build something new," Phil said.
They are building a plant-based café with dishes like chickpea frittatas, marinated tofu steaks and Thai peanut wraps (a popular plate at their former yoga studio and café in Winnipeg).
"Thai peanut sauce is kind of magic when it's done well," Ryann said.
Their daughter Emily is also involved, advising them that the hot chocolate needs a little more agave, and kids should have free refills.
Black charcoal ice cream will come in healthy, unique flavors, the owners said.
"The minute we buy that ice cream machine, it's on," Ryann said.
The café will provide a gathering place for brunch after class, and workshops on how to pack plant-based school lunches. The owners appreciate the extra room to stretch out. In the past, they've stashed sushi ingredients in the office for make-your-own sushi events, and lined up tables along the wall for the plant-based Harvest Feast, an annual fundraiser for Second Harvest Heartland.
When the Orangetheory Fitness space became available next door, they jumped on it.
"Now it has to happen," Phil said.
Phil lives in Kingfield and Ryann lives in Linden Hills. Given their history, people often seek them out for relationship advice.
In moving forward, Phil said they incorporate all the things they've learned on the mat, like how to pause and breathe. Take a breath before saying something stupid, he said. Come back to the Mountain pose, take a breath, and consider a peaceful response.
"All of these things are our yoga practice," Ryann said.
She said they can hit a reset switch, like they do on the mat, and let it all go.
"That's the other thing that's pretty close to magic, other than Thai peanut sauce," she said.
Seed Café is slated to open late this summer.


text:
To direct the Secretary of the Interior to dispose of certain public lands that are subject to mining operations in Pershing County, Nevada, to support sustainable development opportunities for the community in which the mining operations occur through privatization of the lands allowing for productive post-mining land use that provides for economic development opportunities and local government revenues, and for other purposes.
The bill's titles are written by its sponsor.
Sponsor and status
James "Jim" Gibbons
Sponsor. Representative for Nevada's 2nd congressional district. Republican.
Last Updated: Jul 25, 2005
Length: 9 pages
109th Congress (2005–2006)
This bill was introduced on July 25, 2005, in a previous session of Congress, but it did not receive a vote.
Position statements
Jul 25, 2005
Bills and resolutions are referred to committees which debate the bill before possibly sending it on to the whole chamber.
H.R. 3419 (109th) was a bill in the United States Congress.
A bill must be passed by both the House and Senate in identical form and then be signed by the President to become law.
Bills numbers restart every two years. That means there are other bills with the number H.R. 3419. This is the one from the 109th Congress.
This bill was introduced in the 109th Congress, which met from Jan 4, 2005 to Dec 9, 2006. Legislation not enacted by the end of a Congress is cleared from the books.
How to cite this information.
We recommend the following MLA-formatted citation when using the information you see here in academic work:
GovTrack.us. (2020). H.R. 3419 — 109th Congress: Northern Nevada Sustainable Development in Mining Act. Retrieved from https://www.govtrack.us/congress/bills/109/hr3419
"H.R. 3419 — 109th Congress: Northern Nevada Sustainable Development in Mining Act." www.GovTrack.us. 2005. October 30, 2020 <https://www.govtrack.us/congress/bills/109/hr3419>
Northern Nevada Sustainable Development in Mining Act, H.R. 3419, 109th Cong. (2005).
{{cite web
|title=H.R. 3419 (109th)
|accessdate=October 30, 2020
|author=109th Congress (2005)
|date=July 25, 2005
|quote=Northern Nevada Sustainable Development in Mining Act
- show another citation format:
- Blue Book
- Wikipedia Template
Where is this information from?
GovTrack automatically collects legislative information from a variety of governmental and non-governmental sources. This page is sourced primarily from Congress.gov, the official portal of the United States Congress. Congress.gov is generally updated one day after events occur, and so legislative activity shown here may be one day behind. Data via the congress project.


text:
Individual news stories are posted on the APRN news page. You can subscribe to APRN's newsfeeds via email, podcast and RSS.
Commercial Fishermen Rally For Change In Fishing Policies
Shaylon Cochran, KDLL – Kenai
Nearly 200 commercial set net fishermen from the east side of Cook Inlet gathered Friday with their families to rally support for a change in the policies that have allowed just three days of fishing so far this year.
Seward Highway Reopens, Kidnapping Suspect Remains At Large
Josh Edge, APRN – Anchorage
The Seward Highway has been reopened, though the suspect in an alleged armed kidnapping Friday morning remains at large.
Anchorage Police Department spokesperson Dave Parker says APD and State Troopers have suspended the search for the suspect in the Turnagain Pass area of the Seward Highway, but are continuing to pursue leads in the ongoing investigation.
The suspect was discovered rummaging around in the back yard of the victim's grandmother's south Anchorage home, at which point, the suspect allegedly forced the victim into his own pickup truck at gunpoint. According to Parker, the suspect made the victim drive south down the Seward Highway.
"When they reached the Turnagain Pass area, the suspect – who was a white male adult, about 200 pounds, probably in the neighborhood of six feet, with a shaved head and a black and red tattoo on his left shoulder – needed to answer nature's call, so he had the victim stop and the suspect got out of the vehicle to relieve in the woods, taking the key from the ignition," Parker said.
But, Parker says the victim had a spare key hidden in the car, and was able to drive away while the suspect was out of the vehicle…the victim then alerted authorities soon after.
"He actually texted family and then we were able to make phone contact with him when he got into phone range," Parker said.
Parker says that APD was assisted by State Troopers, helicopters, and canine units in the search.
It is believed that the suspect is armed and police are urging motorists to not pick up any hitchhikers in the Turnagain Arm area.
NTSB Releases Report on Plane Crash That Killed Former Lawmaker
Aaron Selbig, KBBI – Homer
Investigators are taking a closer look at the airplane crash on Beluga Lake in Homer that killed former state representative Cheryll Heinze last week. On Monday, officials from the National Transportation Safety Board released a preliminary report on the accident.
New Website Explains State Finances
Dave Donaldson, APRN – Juneau
The first output from a newly established legislative committee was put online this week as members of the House Fiscal Policy Committee kept a commitment to try to let the public know more about state finances.
Subsistence Board Criticized For Not Giving RAC Recommendations Enough Weight
Joaqlin Estus, KNBA – Anchorage
Regional Advisory Councils, or RACs, have criticized the Federal Subsistence Board for not giving their recommendations enough weight. At their meeting Wednesday, board members worked to give due deference to the input of one RAC.
12-Year-Old Fairbanks Boy's Death Ruled An Accident
The Associated Press
Fairbanks police say the death of a 12-year-old boy by gunshot was an apparent accident.
Malik Boykins on Wednesday was visiting a home near Fort Wainwright when he was hit in the head by a bullet from a .40-caliber handgun fired by another 12-year-old boy, who lives at the home.
No adults were home during the incident.
Police say they don't plan to forward criminal charges against any adults. However, spokesman Peyton Merideth says the department is working with the juvenile justice system on possible charges against the boy who fired the gun.
The second boy's name has not been released and juvenile proceedings are not made public.
AK: Home Schooling
Diana Saverin, APRN Contributor
In the tiny and remote communities of southern Baranof Island in southeast, there aren't enough kids for a school. So parents have to home school. And both the parents and the kids try to strike a balance between a more traditional education of reading, writing, and arithmetic, with a more creative approach.
300 Villages: Gakona
Instead of heading to a village this week, we're going to visit the Gakona Lodge and it's owner Greg Marshall and his wife Valerie. The historic roadhouse is located in the Copper River Valley and was established in the early 1900's for travelers on the Eagle/Valdez trail.


text:
Jeff Soo
Jeff Soo took up competitive croquet as a player in 1994, and as a coach, referee, and tournament director in 1996. As a player he has won twelve USCA national championship titles, and has been selected to the United States Croquet Team ten times, three times as Team Captain.
As a tournament director Jeff has managed events at club, state, regional, national, and world level. As a coach he has given clinics and private lessons throughout the southeast and as part of the USCA Schools program. Jeff is a USCA Class I referee and has taught and certified many referees.
Jeff is married to Eileen, a computer scientist and also a keenly competitive croquet player. They live in Durham, NC. When Jeff is not playing or teaching croquet he is a freelance web developer.
Jeff's articles are reproduced here (in alphabetical order). You may need to get the Adobe Acrobat Reader.


text:
Client: Guy's and St Thomas' NHS Foundation Trust
Guy's Tower is an iconic building at London Bridge, and forms the heart of the Guy's Hospital campus. The area around the campus is undergoing significant change, with the Shard being completed but not yet fully occupied and improvements to London Bridge Station ongoing. Patients and visitors were finding access to the hospital becoming increasingly confusing and inconvenient. Tibbalds was appointed to develop a strategy for improving pedestrian access in both the short term, during ongoing construction, and in the longer term once the major developments are operational.
We led a team of consultants, including Aedas R&D and Arups to develop a short-term strategy to improve pedestrian crossing points, open temporary entrances, change traffic movement, including bus only routes, and implement public realm improvements.
This strategy supported a longer-term campus masterplan, which served as the starting point for discussions with Transport for London and supporting bids for further public realm investment into the area.


text:
Pilots and Relays | Differential Pressure Pilot
0.25" Female NPT
Pilot may be installed remotely from the control valve (aka motor valve) (see Control Valves shown in Sections E1 and E2 of the catalog). This pilot is used for maintaining a constant pressure drop across meter systems or to produce a pneumatic output signal when the differential pressure of a system falls below the set differential pressure. (see Control Valves shown in Section E of the catalog)
- Connection Size: 0.25 "
- Connection Type: Female NPT
- Body Material: Steel
- Option Available for NACE MR0175 compliance or certification.
- Pilot Type: Vent
- Max. Working Pressure: 300 psig
- Min. Set Point Pressure: 10 psig
- Max. Set Point Pressure: 300 psig
- Operation: Pneumatic
- Catalog Page: Y:170.2
- Repair Kit P/N: RBL


text:
Cheese Recall
Cheese is a popular food in Wisconsin, and many Wisconsinites tend to consume cheese frequently. A Wisconsin cheesemaker, Hennings Cheese, has issued a recall for some of its cheese.
The recall comes after a pasteurizer malfunctioned. Because of the malfunction, the company cannot be sure that the cheese was properly pasteurized to ensure that it is safe for consumption. This recall is being taken with an abundance of caution, as the company does not want anyone to become sick for consuming unpasteurized cheese. As of now, there have not been any reports of illnesses relating to the recalled cheese.
The cheese that is affected by the recall is Henning's Cheese Colby Jack cheese. The cheese included in the recall was sold startinf April 10, 2017. The cheese affected was sold in the sizes of 1 pound and 2 pound blocks. The cheese will have a date code of 1007 that can be found on a label on the block of cheese. There are about 1000 pounds of cheese affected by the recall.
If you think you may have brought some of the affected cheese, you should check the code on the label before consuming it. If you discover that you own some of the cheese that has been affected by the recall, you should not eat it. You can return the cheese to the place where you bought it or a Henning's Cheese store for a full refund of the purchase price. You can also call Henning's Cheese with additional questions about the recall.
What do you think about this recall?


text:
Ice Block Machine, Cash Cow Or Cash Pit?
We live in the tropics with a lot of heat. Even during the rainy season, the heat is still intense, intense enough to make people to ask for cold drinks.
Therefore, besides the conventional function of food preservation, freezers are needed to cool drinks for people. Since most cannot afford fridges and freezers, it becomes imperative to look for ice blocks for cooling drinks.
Even those who can afford Fridges do not have electricity to power them, no thanks to the epileptic nature of electricity in Nigeria. This is the gap ice block business comes in to fill.
The market is vast in the big cities where the population is on the high side. Cities like Lagos, Ibadan, Kano and Kaduna.
The business also booms where there is high traffic of commuters which again takes us back to the big cities. Petty traders use ice blocks to cool minerals and water for travelers.
Not by any chance is sale of ice blocks limited to this. Other consumers are Hoteliers, beer sellers, clubs and even individuals and governmental organizations when they are having events.
Ice block can come out in any shape. In fact, the block will take the shape of the container when it is done.
But generally there are two types in the market. They are the rectangular ones which are made by just pouring water into rectangular holes constructed in the machines. The other is the tied type which is made by pouring water into transparent polythene bags.
Here you have the big type and the small type. The rectangular type is more popular and it commands higher price than the tied type even if when they are of the same size. Nobody can explain why but that is what is happening in the market.
There are two sources to get ice block making machines. You either get those built locally or the imported ones. To get information on imported ones just goggle ice+block+making+machine.
As for locally made machines you find them in Ibadan and Lagos. However, Ibadan appears to command a higher percentage of the makers.
Prices differ from maker to maker and this depends on many factors. Some of the factors are
1.Size of blocks
- Quantity of blocks
- No of compressors (Two machines of the same size with different number of compressors will differ in price.)
- Size of compressors(2HP, 2.5HP or even 3HP)
- Whether the compressors are used(tokuno) or new.
- Beauty
7.With or without Stabilizers
The most common machine by local manufacturers is the one built to make 25 rectangular blocks and this goes for about N250,000 depending on who is making it and what materials he is using.
You either sell directly to users or retailers who come to your plant to pack them and sell to users. If you are selling to users it means you will need a depot and freezers to keep the blocks in shape till they are sold.
This of course means more expenses on transportation, space, and cost of labour. You will have to do your cost and benefit analysis to decide, but if you ask me I will say let the retailers carry from your plant and reduce your worries.
But you must be current of prices otherwise the retailers will cut your throat.
During the dry season the price is naturally higher. During this period you can sell a block to the retailers for as high as N100 but during the raining season they may refuse to take it for N40.
Weather, season and competition affect your pricing. You ask questions and feel the market to arrive at your price and this may vary on daily basis.
You just have to be current.
Check out Profitable Small &Medium Businesses For Nigerians
This is the crux of the matter. Is this business profitable? Yes if current is stable. Even if you are selling a block for N20 you cannot lose. With stable current your machine will freeze more quickly and the machine will last longer.
Your raw material is water and all you pay for is the labor that fills in the water and harvest the blocks and of course electricity bill which may not amount to much.
No business can be better.
However if electricity is erratic as we have in many cities these days, it becomes another kettle of fish altogether. If you freeze for 10 hours and the light goes for another 24 hours you get back to square one and remember your compressors have been running.
Not only that, erratic supply of electricity shortens the life of your compressors.
We shall see presently. You add a Chinese 15KV generator to take your installation cost to about N400,000. You power with diesel at N160 per litre and run for 20 hours or even 24 hours. This works out at N3200 for fuel alone for one production.
Let us assume you sell for N50 per block, your 25 blocks machine will gross N1250. That is a disaster.
OK, you sell at N100per block which is highly unrealistic unless it is hell hot; your machine will gross N2500. Even at that it is bad business. You have not built in the cost of labour, depreciation for the machine, maintenance for the generator and other inputs.
The only way this business can be profitable with generator is if you have stable electricity and use the generator to fill in the little disruptions.
Or you are loaded and buy a big generator to power a battery of machines and this will run into millions. But, how much block do you make to sell and recoup millions and how quickly.
I may be wrong but my verdict is this, as a struggling man, do not venture into ice block machine if electricity is not stable in your place no matter how fertile the market is.
There are other profitable Low Capital Business in Nigeria. Visit here


text:
Sensex tumbles 292 points to end at 4-month low
Question marks over government's reform initiatives, global woes erode Rs 1 lakh crore in investor wealth.business Updated: Apr 04, 2013, 23:28 IST
Political uncertainty at the Centre raising question marks over the government's reforms initiatives and weak macro-economic data, combined with doubts about a global recovery, are dragging the Indian markets down. Expectations of muted earnings from Indian companies in the fourth quarter are also affecting sentiments.
Falling for the second day, the benchmark Sensex on Thursday plummeted nearly 292 points, or 1.6%, to end at four-month low of 18,509.7, eroding around R1 lakh crore in investor wealth. The index has fallen 531 points in the last two trading sessions.
The broader National Stock Exchange Nifty also plunged below 5,600 level by losing 98 points, or 1.7%, to end at 5,575.
"Political uncertainty at the time when macro-economic data is showing that the Indian economy is not in good shape is taking toll on investors' confidence," said P Phani Sekhar, analyst, Angel Broking. "Investors are concerned that the reforms process may be stalled if the government falls. Expectations of weak corporate earnings have also made investors nervous."
India's economic growth outlook is still shaky with the current account deficit coming at 6.7% of gross domestic product.
Foreign institutional investors or FIIs, who sold shares worth around R368 crore on Wednesday according to provisional data from stock exchanges, were net sellers.
"Technology stocks were trading lower on weak US economic data while capital goods stocks were trading lower on concerns that the slowdown may restrict new orders," said Alex Mathews, research head, Geojit BNP Paribas Financial Services.
Going ahead, the market will watch corporate earnings. "Investors will watch result numbers of pharma and IT sectors, which have been holding the market firm," said Sekhar.


text:
Hilton Garden Inn Houston Cypress Station160 Wagon Point, Houston, Texas, 77090, USA TEL: +1-281-929-0111 FAX: +1-281-929-0112
Things To Do
Get away from the busy city at our peaceful Hilton Garden Inn Houston Cypress Station hotel. Located just north of downtown Houston and close to George Bush Intercontinental Airport (IAH), you'll be close to everything you need. Do some bird-watching and hiking at the Armand Bayou Nature Center or the Houston Arboretum and Nature Center in Memorial Park.
Go downtown and watch a major sporting event at NRG Stadium, Toyota Center, Minute Maid Park or BBVA Compass Stadium. Relax with a round of golf at the nearby Champions Golf Course or try a round of shopping at the Woodlands Market Street or the Galleria Mall. So many attractions to choose from, near the hotel.
Houston Zoo
The zoo is home to more than 6,000 exotic animals representing more than 800 species.Houston Zoo
BBVA Compass Stadium
Catch a soccer game at the 22,000 seat venue.BBVA Compass Stadium
Downtown Aquarium
Home to over 200 species of aquatic life from around the globe.Downtown Aquarium
Minute Maid Park
Come watch the Houston Astros play baseball.Minute Maid Park
NRG Stadium
Catch a football game and watch the Houston Texans.NRG Stadium
Galleria Mall
With 375 stores, The Galleria is the fourth largest shopping center in the nation.Galleria Mall


text:
Service Cats Getting Kitty Ready For An Outing
Welcome to another miserable Monday. It's time fur another Educational Service Cat Posty. Our last few posts have dealt with training specifics dat are good fur all cats and kittens, not just Service Cats; and today's post will be no different. We got some great questions dat we'll be dealin' with fur da next couple of weeks, but 'member to ask your questions, so we can get them answered too. Now, ifin you've missed any of da posts in this series you can ketch up by clickin' da links below. We do suggest befur you begin trainin' kitty to purrform any task dat you start with mommy's Foundation which you can read 'bout in Part 2, Training Foundations. As with all our educational posts da followin' will be in human English, so get your coffee, note pad and let's get to it.
Part 1: https://dezizworld.wordpress.com/2016/06/20/service-cat-what-to-look-for/
Part 2: https://dezizworld.wordpress.com/2016/06/27/service-cat-training-foundation/
Part 3: https://dezizworld.wordpress.com/2016/07/06/service-cats-how-to-train-kitty-to-massage-without-claws/
Part 4: https://dezizworld.wordpress.com/2016/07/11/service-cats-smelling-disease/
Part 5: https://dezizworld.wordpress.com/2016/07/18/service-cats-do-you-need-one/
Part 6: https://dezizworld.wordpress.com/2016/07/25/service-cats-who-bears-the-cost/
Part 7: https://dezizworld.wordpress.com/2016/08/01/service-cats-housing-laws-and-exceptions/
Part 8: https://dezizworld.wordpress.com/2016/08/08/service-cats-accommodations-and-common-sense/
Part 9: https://dezizworld.wordpress.com/2016/08/15/service-cats-what-happens-when-the-handler-dies/
Part 10: https://dezizworld.wordpress.com/2016/08/22/service-cats-proper-training-methods/
Part 11: https://dezizworld.wordpress.com/2016/08/29/service-cats-proper-training-methods-part-2/
Part 12: https://dezizworld.wordpress.com/2016/09/05/service-cats-whats-the-easiest-task-to-train/
Now that you're all caught up, let's get to our question. Our friends from Toby's Wrecking Crew wanted to know how to get a kitty ready for something like BlogPaws. And what they are asking is how do you get a kitty adjusted to a lot of people, noise and other animals, unruly doggies to be exact?. This is a great question. It most certainly applies to Service Kitties who must be accommodating to any situation, but it's also good for all kitties. You never know what the future holds, or what situation you may find yourself in. For instance, you find yourself having to evacuate your home and go to a shelter full of people and other animals, a kitty who is comfortable in this setting is certainly going to be easier to deal with during this time of crisis. Or heaven forbid, you have to take kitty to an emergency vet during a busy night. Again, a kitty that is comfortable with noise and commotion, is going to be much calmer than one who isn't. Now that we've pointed out why all cats should be trained for commotion, let's explain how it's done. Remember, It's NEVER too late to train kitty.
Of course this is something mommy starts on immediately with the new kitty. But remember that first Service Cat mommy had? Well she wasn't a kitten when she was trained, so it can be done. The first thing you want to do is make kitty as comfortable and safe as possible. So here comes the harness and leash training. We do recommend a stroller for these kinds of outings as opposed to a carrier, but keeping a carrier on hand is also a good option. (See the photo below of sis Lexi in her carrier on top of the stroller in the car.) Even in a closed stroller, kitty should be leashed and tethered in. We're going to take this from the start. Buy a proper fitting harness. Mommy prefers the ones with material that have closures around the neck and body, like you see in the photos. We prefer them as well. Cats are more comfortable being wrapped up. So we recommend staying away from the strappy cat harnesses and go straight to the dog department. You can also order online, there are a lot of great companies that make these harnesses for kitties. You'll need a leash that's not to heavy. Remember, the typical kitty weighs less than 20 pounds so they're not going to break even the skinniest of leashes. Now that you have the proper tools, you can start training kitty. We recommend keeping the stroller and carrier out at all times and open for kitty to use for sleep or play.
Harness Training:
Each day put harness on kitty for about 15 minutes and walk away. Initially kitty may go limp and fall over. Don't Worry, kitty is fine. Do Not remove harness or kitty has trained you. Only take the harness off after 15 minutes. And take it off even if kitty seems okay with it. You don't want kitty to over do it initially. Do this twice a day for a few days and then increase the amount of time the harness stays on once kitty actually gets up off the floor. The amount of time this will take will vary from cat to cat, so we can't give you an exact number of days or weeks, but once kitty is comfortable in the harness you can now add the leash. Please, Do Not Rush Kitty. Take your time, this will mean success. And remember, all of this is being done in the house. Kitty hasn't set one foot outside. Okay, you've added the leash to the harness. This should be done the same way as the harness, start with 15 minutes twice a day and increase until kitty is comfortable. During this time, please be careful as kitty will be dragging a leash and this could be a trip hazard for all humans. And keep an eye on kitty if opening any doors or cabinets, etc.. Once kitty is comfortable with the harness and the leash, it's time to add you to the mix. Pick up the leash and let kitty take you for a walk. At this time you may venture outside if you want. This is also the time to start taking kitty with you in public.
Going Public:
Even tho' kitty is now harness and leash trained, it's no comparison for a stroller or carrier. Harness and leash kitty and tether in to stroller for your outing. Of course a pet store is the obvious choice, we like to go places like parks, banks, pharmacies, post offices, etc.. (me's visitin' the pharmacy in the photo below) Try going for a stroll in the park. You'll likely encounter a few dogs and curious people. Your bank, or a small town bank will most likely say nothing and will expose kitty to a lot of lookey loo's (curious people wanting to see kitty) During these outings as kitty gets more comfortable you can open the stroller and let people pet kitty and let kitty sniff them. You can also allow kitty to walk around a little if permitted since kitty is harnessed and leashed. These outings expose kitty to a small number of people/animals and noise, but prepare them for the commotion of a crowd. Now that kitty is comfortable in these small settings it's time for the big crowds. Again, the pet store during an adoption event is a great place. A school football/basketball/baseball game is a great place. A local parade or flea market is another great option. Look around your area, you'll find several great options. The more exposure you can give kitty the better.
The following instructions are for BlogPaws only, and something mommy regrets having to say, and hasn't yet done for us. Altho' me's been thru it already so me doesn't need the training. Find a "yard" that's protected by large guard dogs and take kitty to the fence. Please be sure kitty is tethered into their stroller and that the stroller is closed and secure!!! Do Not leave kittys view at this time. The dogs will charge the fence and thus the stroller, barking and growling and baring teeth. The action you want from kitty is to quietly go to the back of the stroller and lie down. Remove kitty from the situation. If kittys response is to try and fight, remove them immediately and try again another time. Not all the dogs at BlogPaws are this way, but there are a few and their humans have no respect for anyone. Of course, you never know when you walk out your front door what you might encounter. Even tho' we are Service Cats and are taught not to react, sis Lexi and me both did hiss at a few doggies that charged us. But we did then return to the back of the stroller and lie down as we should have. So if kitty hisses and lays down, you're alright. You just don't want kitty going crazy and trying to escape the stroller. They need to feel completely safe with you in their stroller.
Remember, Training is Repetition. Don't rush any step or you'll fail. If you're planning for a specific event, stat your training early and keep at it. Don't let a day go by without training. Once kitty is comfortable in all these situations, reward them by taking them out and about just because. Mommy says that training is also forever. Meaning you have to keep exposing kitty to these situations or they'll grow complacent. Make it a point to take kitty out at least once a week. Mommy tries to take us for a stroll every week, weather permitting, and for a drive at least once a week, even if it's only around the block. Most importantly, have fun. You want kitty to find this as enjoyable as you. Big snarly dogs and all we would love to go back to BlogPaws. We would be better prepared this time around. And we've got our paws crossed we make it there again.
We're gonna wrap it up for now. Hope this helps ya'. Ifin ya' have any additional questions or comments 'bout todays topic or another training tip you're interested in, purrlease say so in da comments below. You can also email us using our contact page which is completely purrivate. All this talk 'bout strolls has me wantin' to go fur one. Gotta run now.
Do you (your kitty) like to go on outings?
Would you like (your kitty) to go on outings?
We're curious how many kitties are already harness trained?
Till da next time……………………………….Be Blest!!!
Luv and Hugs and Kitty Kisses
Deztinee and RaenaBelle
Posted on September 12, 2016, in Educational, Human Speak, Service Cats, Training and tagged #RagdollCats, #ServiceCats, Deztinee, Educational, English, Human speak, RaenaBelle, RagdollKittens. Bookmark the permalink. 44 Comments.
We took Ringo on many outings (in his carrier, we don't have a stroller)–the pet store, hubby's workplace when it was quiet, the pet bakery, etc. He is really a confident cat and I wish I'd done more of it with Sadie when she was younger. It makes a difference. (Our late Tucker had a lot of outings when he was young, when I was single and took him everywhere. He too was a well-adjusted kitty.)
Pingback: Service Cats: Tips For Moving With Cats | Deziz World
Pingback: Service Cats: Kitten/Cat Proofing Your Home Pt. 4 | Deziz World
Pingback: Service Cats: How To Deal With An Attack By An Unleashed Animal | Deziz World
Pingback: Service Cats: 6 Tips To Keep Kitty Stress Free While Going To the VET | Deziz World
Pingback: Service Cats: What Age Should Kitty Be Altered & Preparing For A Trip To The VET | Deziz World


text:
Collection Search
A Dangerous Division
- Creator:
- Lloyd Ostendorf
- Location:
- Dayton, Ohio
- Origin Date:
- 1968
- Materials:
- paper, pen and ink
- Measurements:
- overall: 14 1/2 in x 11 1/4 in
- Item ID:
- 71.2009.081.0665
- Holding Institution:
- Lincoln Financial Foundation Collection, courtesy of the Indiana State Museum
- Available for Viewing:
- No
- Category:
- Magazines & Newspapers
This is an original sketch of a political cartoon. The cartoon features Abraham Lincoln, depicted from the waist up, holding a miniature version of the Capitol Building in his hands. The building is cracking and spilt ting directly down the middle. Above the building are two birds who appear to be fighting and squawking at one another. One bird is white, and the other one is dark. The white bird is a bit smaller and seems more benevolent. The darker bird is larger and more threatening. The year of production, 1968, is handwritten in blue pencil in the bottom proper left corner. The title, also in blue pencil appears in the top margin. The title has actually been written twice, one line on top of the other, and the upper version has been crossed out. The artist signed the cartoon with his name and the year in which it was made in the folds of Lincoln's sleeve. "544" is handwritten in pencil in the top proper left corner. "OC Cut Card Made 11/85" is handwritten in pencil on the reverse side in the bottom proper left corner.
Explore the Collection
Curated Groupings
- The Generals of the Civil War
- A Look Into the Honest Salesman
- Lincoln in Cartoons: Through the Years
- Robert Todd Lincoln
- The Booth Mummy
- We Mourn a Martyred Father
- "O moody, tearful night!"
- This Cruel War Is Over
- A Case of Mistaken Identity
- The Gettysburg Address
- The Lincolns of Springfield, Illinois
- The 1860 Election
- A Civil War Soldier
- The Election of 1864
- The Emancipation Proclamation
- The Widow Lincoln
- A Superb Affair
- The Lincoln Family Album


text:
Global Lingo London love to get involved in walking, baking, more baking, and even running like Wally for charity and, after raising over £1,600 for the National Literacy Trust in 2014, they are looking forward to challenging themselves further and raising even more money in 2015.
The charity choice for this year is The Brain Tumour Charity. They are the UK's largest dedicated brain tumour charity, and their stated mission is to fight brain tumours on all fronts.
Brain tumours are the biggest cancer killer of children and adults under 40 in the UK. Quite often the signs and symptoms of brain tumours mimic other more common illnesses, like meningitis, and more often than not they are overlooked until it's too late.
Research offers the only real hope of improvements in the diagnosis, treatment and management of brain tumours. Sadly, figures show that it is seriously underfunded: brain tumour research receives less than 1% (0.7%) of national cancer research spending in the UK. The Brain Tumour Charity funds scientific and clinical research into brain tumours and offers support and information to those affected, all while raising awareness and influencing policy.
Wear a Hat Day
The leading brain tumour awareness event in the UK is set for tomorrow, 27th of March, and will involve plenty of hats! Simply put, people wear a hat that day to show support for The Brain Tumour Research's aim: to fund brain tumour research in the same way as leukaemia, breast and other cancers.
The event encourages people to share their stories about brain tumour, whether they are personal experiences or are about someone who has been affected by such a tumour. These stories are truly inspirational and the entire event has caught the public attention already.
No-one can prepare you for how devastating it is when a loved one becomes ill and when Jack was diagnosed with a brain tumour at the age of four, it was devastating for us all. Jack is now an accomplished young man and together we want to help raise awareness of the need for further research into such a distressing form of cancer. My family was lucky but 16,000 people are diagnosed with a brain tumour every year and there desperately needs to be further funding in place to one day find a cure. My family will certainly be showing their support for Brain Tumour Research and its member charities on Wear a Hat Day and hope to see lots of people doing the same.
Sheila Hancock, Actress and author
Find more such testimonies here.
The Global Lingo London team are happy to be involved in this event, lured at first glance by the chance of wearing fabulous hats during working hours, but being aware nonetheless of the importance of the cause and donating a small amount to their Just Giving page. We invite you to do the same.
Pictures or it never happened!
Since we live in the "selfie" era, we are anxious to see everyone in the London office wearing their magnificent head-wear. So, watch this space for their pictures!
Until then, have a consolation prize with this cutie from a previous edition:
Update March 27th
Find out more about the signature brooches in the header photo here.


text:
Versión en EspañolContentsAlleged DiscrepanciesArticle ReprintsAudio ResourcesBible BulletsDarwin Day DebateDecisive DesignsE-Books"In the News"Reason & RevelationResearch ArticlesScripturally SpeakingSensible ScienceResourcesDiscovery for KidsExamine the EvidenceHome Study CoursesFeedbackEBGlobalA.P. InformationAbout APContact APCopyright StatementHelp APPrivacy StatementSpeaking SchedulesA.P. Scientists and Auxiliary WritersUsage Guidelines
Due to the brief nature of the debate, several of the assertions made by atheist Dan Barker could not be answered in the allotted time. This section of the A.P. Web site provides articles that respond specifically to these assertions. Additional articles that address various statements that Barker made during the debate will be added below as they are produced.


text:
This image, acquired on 28 October 2011 by Envisat, shows central Saudi Arabia on the arid Arabian Peninsula.
Most of Saudi Arabia has a desert climate and experiences extremely high temperatures, sometimes reaching up to 50°C in the summer.
While there are virtually no permanent rivers or lakes in the country, large valleys called 'wadis' fill with water during times of heavy rain. Most settlements are located along these valleys.
The area pictured is on the central plateau, Nejd, which slopes downwards from west to east.
The dark circle near the centre of the image is the capital city of Riyadh, the nation's legislative, financial administrative, diplomatic and commercial hub.
Despite the dry climate, large irrigated crops have been established in the area west of the capital. The irrigation of the fields largely depends on fossil water that was stored underground when the area received more rain several thousand years ago.
This image was acquired by the Medium Resolution Imaging Spectrometer on Envisat.