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from $0.70
These Oval Silicone Wristbands NXP MIFARE 1K combine RFID technology within an attractive and practical housing. They are robust, waterproof (the module is a sealed electronic unit) and are easily worn around the wrist. These Small Oval Silicone Wristbands are 53mm diameter (standard), however, a slightly bigger wrist size of 55mm can be obtained on request. However there is Medium (60mm) and Large (64mm) size options available to purchase today.
Silicone wristbands are fantastic for events and promotions,available in a range of vibrant colours which can be branded with text and logos.
Materials: Silcone
Frequency: 13.56 MHz
Memory Size: 1 KB
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Size: 53mm / 55mm / 60mm / 64mm
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2. If don't have template, please go to our template page to download it
You can enjoy discount when you order more,
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text: Back in stock: our popular booklet, For Your Protection: Peace Bonds and Family Law Protection Orders, is again available from Crown Publications. We've reprinted both the English and the Punjabi versions with minor improvements: we've added information about the court forms needed for a protection order and also that Supreme Court fees can sometimes be waived. For Your Protection explains how and when people can apply for peace bonds and family law protection orders, and what the differences are between them. The booklet is also available in Chinese (simplified and traditional) and French (online only).

subset: commoncrawl


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Lovely rental in central Annecy, quiet area, private car park in a private courtyard. Brand new rental with quality fittings. Equipped kitchen, large bathroom with wet room, pellet stove.
Premium bedding, basement TV room with leather sofa bed. Window and door fitted with electric shutters and curtains.Free bike lending, free wifi, 2 TV...
All year round.
18 rue Louis Chaumontel
74000 Annecy
Phone : 06 51 56 57 92
Latitude : 45.903923
Longitude : 6.119726
Town location
Week: 390 to 790 €.
Tax not included.
Or with Lac Annecy ID


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Dr. Adriann (Waltenbaugh) Hooks DMD
Dr. Hooks is a lifelong resident of the Kittanning area. She graduated from Kittanning High School in 2001. She then received her Bachelors Degree from Slippery Rock University in Biology with a minor in Chemistry. Dr. Hooks attended the University of Pittsburgh School of Dental Medicine where she received her Doctorate in Dental Medicine, graduating summa cum laude. She has been practicing in the area since graduating in 2009. She enjoys traveling and loves to spend her free time with her two sons and husband. Dr. Hooks' favorite part of her job is being able to develop personal relationships with her patients and staff. Owning her own practice has given Dr. Hooks the opportunity to provide her community with honest, affordable, quality dentistry.
Rose Caratelli RDH
Dental Hygenist
Rose graduated from West Liberty State College in 1979. She has been working in this practice for 32 years. Rose's favorite part of her job is interacting with patients and educating them about good dental care. In her spare time, Rose enjoys working out, cooking, reading, and spending time with her family and friends.
Heather Hooks RDH
Dental Hygenist
Heather received her training from Tri-State Business Institute where she earned a certificate in dental assisting and an associates degree in dental hygiene. Heather enjoys educating her patients on home oral care and the effects that a person's mouth has on their overall health. Heather loves spending time with friends and family and watching crime shows on TV.
Rose Debnar
Front Desk
Rose has been employed at this practice for 16 years. Rose works at the front desk and her responsibilties mainly consist of financial arranging, insurance claim processing, daily and monthly reports, and scheduling patients. She has completed continuing education courses in computer technology, business, and marketing. Rose has also attended numerous seminars in dental education and dental insurance.,,Rose's favorite part of her job is having personal contact with the patients and the satisfaction of working with an office that sincerely wants their patients to maintain good dental hygiene which results in an overall healthier individual.,,Rose enjoys traveling with her husband, Emil, to various places including Europe and the Middle East, especially the ancient Christian and biblical sites, as well as trying new cuisines and recipes from all over the world. She also loves to cook, especially with fresh fruits and vegetables.
Pam Levcik
Pam has worked as a receptionist in different dental practices for over 22 years. She has worked in this practice for the past three years. Pam enjoys interacting with each patient and treating them in a conscientious and professional manner, knowing that each dental appointment begins with her. Her hobbies consist of walking, flower gardening, knitting, reading, and baking.
Carole Mikula
Dental Assistant
Carole graduated from The Career Training Academy in New Kensington in 1994. She has enjoyed her time at this practice since she began working as an assistant for Dr. Martin in 1997. Carole loves watching and doing different dental procedures and learning new things. In her spare time, Carole enjoys doing crafts and making wreaths and different basket centerpieces.


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In the fall when we put things away, it was always "What do we do with the sand from the sandbox?" Well, I assess what areas of my veggie and flower garden soil needs some soil treatment and divide out the sand box to the garden needing it most. Eliminates the spring work of finding sand to use, as well as putting the sand box away.
Add your voice! Click below to comment. ThriftyFun is powered by your wisdom!
Adding some sand to clay soil turns it to "concrete" basically, so it really depends on your soil type if it is a good idea. If you add enough sand, it will lighten clay soil, but adding small amounts doesn't help at all.
I was going to comment similarly to ginnee. Sand + Clay = Bricks. We're chock full of clay in Oklahoma, so we need a lot of organic material instead.
Add your voice! Click below to comment. ThriftyFun is powered by your wisdom!


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You will find a vast selection of hotels on the Maltese Islands, ranging from the larger internationally-branded resorts to the small family-run boutique hotels; from coastal establishments located within walking distance from the beach, to rustic residences situated village centres, to city hotels.
Malta has numerous luxurious 5 and 4 star hotels that cater to the needs of any individual or group. We have a total of 14 Five-star hotels, which can together accommodate more than 5,500 delegates and all of which are well aware of the discerning needs of conference and incentive travellers. These establishments all have in-house meeting facilities and many of them are clustered within walking distance of each other, enabling event organisers to split up large groups into different properties if need be. The 40-plus 4 star hotels on the Islands provide an additional 14,000 beds.


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The ability to create explosives made of hair. Sub-power of Hair Attacks. Variation of Organic Bomb Generation. Combination of Volatile Constructs and Hair Manipulation.
Also Called
- Comakinetic Bomb Generation
- Comakinetic Grenade Generation
- Hair Bomb Creation
- Hair Grenade Generation
The user can create and launch bombs, explosives and other volatile constructs composed of hair, which can have various effects on the target.
Known Users
- Flare Corona (Fairy Tail)
- Yugito Nii (Naruto)
Community content is available under CC-BY-SA unless otherwise noted.


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Oh I love them all, but especially that bird, and the green colour of the yachting scene. I agree — there is nothing lovelier to the touch than well worn linen or cotton. I know this as I have been doing patchwork all weekend using a combination of worn, and not-so-worn. Hope you're well, Jo!
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Before and after: Left William on 5th January 2015 to the right William 30th July 2015
At first glance, it's hard to believe William Davis' Tau Ora statistics.
At a time when many Te Wānanga o Aotearoa kaimahi are battling the cold and falling motivation levels to up their Tau Ora points, William clocks an average of between 40,000 and 50,000 steps most days of the week.
"I was doing so much so the first one (pedometer) has run out, it kind of died on me someone else gave me another pedometer and I also have one on my phone," said William, kaiako for the Level 4 Māori Performing Arts Certificate/ Māori Theatre programme in Mangere.
Throw in to his fitness mix Monday to Friday gym workouts – that start at 5.30am and last nearly three hours.
Added to this are the 60 to 90-minute training sessions in the afternoons with fellow kaimahi Aaron Henare and Riki Ramanui, theatre/kapa haka/mau rākau/ti rākau/ exercises and warm-ups with his tauira three days a week.
On top of all of this is at least 13km of walking every day.
You can soon see why William's daily Tau Ora points haul regularly tips the 80,000-mark.
The acclaimed actor has amassed more than 8,000,000 Tau Ora points so far this year – more than 1,000,000 more from his nearest rival.
And for good measure, his Tau Ora Will's Wild Walkers group are also leading the team competition.
He admits his approach to our organisation's Wellbeing programme that was designed to move kaimahi towards wellness is possibly "extreme".
But the results he's had since starting this year's programme can't be denied - with his weight down more than 15kg.
"I also measured 119cm around the waist but am now 96 cm. My chest has gone down from 120cm to 108cm and my hips are down from 120cm to 105cm."
"I think I'm driven, I'm a bit of a madman."
Te Wānanga o Aotearoa Wellness Advisor Tuihana Ohia said Tau Ora, now in its seventh year, is continuing to garner significant interest after winning the Supreme Award at The New Zealand Workplace Health and Safety Awards earlier this year.
She said both the Accident Compensation Corporation and telecommunications provider 2 Degrees had both shown major interest and had met for talks about incorporating elements of Tau Ora in their own kaimahi wellness programmes and exploring the possibility of partnering on a number of initiatives.
"In 16 years of the awards it was the first time that a wellness programme had won the supreme award," said Tuihana.
While Tau Ora is designed to get kaimahi involved in healthier habits during the cold winter months, Tuihana said it wasn't uncommon to see a drop off in activity around that time.
"Things usually start with a bit of a roar and then drop off when it gets colder but then the warmer months come along and people are naturally more active."
The Tau Ora competition continues until October 17.
Tau Ora was launched in 2009 and has been hugely successful with 70 percent of wānanga kaimahi taking part in the initiative.
It's based on the idea of five team members supporting each other to overcome challenges and achieve their personal wellbeing goals.
Tau Ora is holding its annual awards at the Wintec Atrium in Hamilton on November 27.


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Title: The Haunting of Hill House Author: Shirley Jackson Published: 1959 Publisher: Viking Audiobook Narrator: Bernadette Dunne — I decided recently to put together an extremely non-definitive, definitive list of the "scariest novels ever written". I put this together by googling that quoted search term, and then using 28 different lists from (seemingly) reputable sources,…
Book Review – Branden Chowen on The Haunting of Hill House by Shirley Jackson
October 26, 2018 Branden Chowen 0 Comment
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Listings in Home Accessories and Aromatherapy
Luna Textiles
Luna Textiles is a family-run mill in the UK, manufacturing a broad range of natural fibre duvets, pillows, mattress toppers and protectors using first grade alpaca and finest cottons.
United Kingdom
House of Marley
in Accessories, Home Accessories
House of Marley superior sound, home and personal audio systems - headphones, Bluetooth® speakers, and lifestyle accessories. Our high-performance audio products are engineered to deliver the Marley "Signature Sound".
United Kingdom
The Rose Tree
in Home Accessories, Facial Skincare, Bodycare
The Rose Tree is a multiple award winning British beauty brand with a focus on extraordinary organic products for body and soul. Designed for modern women who want to look and feel amazing in today's frantic-paced lifestyle.
United Kingdom
Shui Me
Shui Me is a multi-award winning range of luxury, organic, long lasting aromatherapy reed diffusers that harness the aromatic power of plants to help you achieve a more balanced mood. Made from 100% organic essential oils & only natural ingredients.
United Kingdom
Sofia Latif
in Facial Skincare, Aromatherapy
Sofia Latif believes oils have the power to create healthy and strong skin. Luxuriate in a skincare routine using precious, waterless blends, which are made with 100% pure plant oils & nothing else – all selected for their nourishing powers.
United Kingdom
Naked Flooring
Naked Flooring supplies natural carpets & rugs that are durable, stylish, ethical and affordable. Plant fibre carpets, made from Sisal, Seagrass, Jute and Coir are Naked Flooring's speciality and the company also has a huge range of 100% wool carpets
16 Clifton Park
Dyrham Lodge
Bristol BS8 3BY
United Kingdom
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in Kitchen, Home Accessories, Dining
Huskup is a reusable coffee cup made from rice husk, not plastic. Biodegradable, durable and free from plastic, Huskup is tackling the UK's disposable culture one coffee at a time with a brand new reusable cup made from rice husks.
United Kingdom
in Natural Health, Home Accessories
Blushield is an active EMF protection technology designed to protect users from the health effects of man-made electromagnetic fields. It's available in a wide variety of models to suit all lifestyles.
United Kingdom
Laura's Loom
Beautiful, exclusive throws woven from best quality British wool sourced directly from the farmer.
Farfield Mill
Garsdale Road
Sedbergh, Cumbria LA10 5LW
United Kingdom
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We are Bamboodu, an online store and community promoting healthy live style, ecology and zero waste. We believe that our organic and biodegradable products can directly reduce the amount of plastic ending up in an ocean, river, and landfill.
United Kingdom


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March 6, 2017
By Robby Hamman
NOTRE DAME, Ind. – Notre Dame men's lacrosse senior defender Garrett Epple has been named the ACC co-Defensive Player of the Week for the second consecutive week after he helped lock down No. 1 Maryland's offense in the 5-4 win on Saturday at Arlotta Stadium.
The award marks the second career ACC Defensive Player of the Week honor for Epple, who was pegged by Inside Lacrosse as a Preseason First Team All-American. Epple also garnered Preseason All-ACC accolades in 2017 and was an honorable mention All-American after his sophomore season.
Epple led the Notre Dame defense to one of the most impressive performances of the season in Saturday's victory over Maryland, as the Irish held the Terrapins to just four goals. The senior disrupted the Maryland attack all game, as he tied his career high in caused turnovers with four and also led the Irish in ground balls with six.
The four goals are the fewest Maryland has scored all season with its previous season low being 10 goals in a game. The four goals are the lowest total Notre Dame has allowed this season and the fewest since defeating Maryland last season by a score of 9-4 on March 5, 2016 in Costa Mesa, California. The Notre Dame defense is tied for first in the ACC in goals allowed per game, giving up an average of just 6.33 over the course of three games.
Epple currently leads the ACC and ranks third in the nation in turnovers caused per game with an average of 2.67.
The Irish travel west for another marquee matchup. Notre Dame squares off with Denver at 3 p.m. ET on Sunday, March 12 in Denver. The game will be broadcast on Altitude TV.
The University of Notre Dame men's lacrosse team pursues excellence on and off the field through the three pillars in which the program is built: Character, Culture & Community. These three foundational values guide the promise of the program, which is to provide its student-athletes with the most compelling and enriching experience in all of college athletics. Through academics, competition, service and travel, the program aims to immerse its players in situations that enhance their student-athlete experience to help them become the people, students and teammates they aspire to be.
Follow the Notre Dame men's lacrosse team on social media and the Internet …
Click here to follow ND Lacrosse on Twitter
Click here to follow ND Lacrosse Coaching Tips on Twitter
Click here to learn more about the ND Lacrosse Experience on Twitter
Click here to like ND Lacrosse on Facebook
Click here to subscribe to NDLaxTV YouTube Channel
Click here to read the latest news on ND Lacrosse
Click here to learn more about ND Lacrosse Camps
Click here to learn about ND Lacrosse community service on Twitter
Robby Hamman joined Fighting Irish Media as athletics communications assistant director in September 2016, coordinating efforts for the volleyball and men's lacrosse program. Hamman is a 2010 graduate of Bethel College, where he earned bachelor's degrees in sport management and business administration, and earned his master's degree in business administration from Defiance College in 2013.


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Joined a new group yesterday that I have wanted to join for quite some time. I know that there is a lot of creative 'stuff' floating around in my head that I just need to let out. Beyond the Borders (BTB) is a contemporary art quilt group they encourage everyone to "embrace creativity while coloring outside the lines of traditional quilting".
I was especially inspired by the coffee filters that Liz produced and immediatly tried to dye some coffee filters myself! Well, first of all I took the coffee filter out of the coffee pot from this morning, thinking that was a good start. I proceded to dump the grounds out of the filter and rinsed it. Duh, all the dark colors came out. I put the filter in a plastic container, and poured a little bit of the leftover coffee from the pot on the filter. I let that soak over night. Nothing. Same off white color as when I rinsed it.
To make a long story short, after checking the internet, maybe I should be using 'Instant coffee' . The other tip I found was that the dark the roast, the darker the stain. (I'm using HEB San Antonio blend- should that give a red cast?? LOL) .
Today, I went over to Wally world and bought a small jar of instant coffee. Tomorrow, we are going to try the spritzing method. I found this recipe at this Folk Art blog:
mix 5 tablespoons of instant coffee crystals in half a cup of hot water.
Add a few drops of vanilla extract to for a great scent
Using an old sponge, blot the dye on the fabric, adding more until you get the desired look.
She will then put in a 200 degree oven for about 10 minutes to facilitate drying.


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SINGAPORE - A woman who offered a $10,000 bribe to stop a police officer from investigating her involvement in possible offences under the Women's Charter was sentenced to four weeks' jail on Monday (Oct 3).
Zhu Kuai, 42, unemployed, admitted to offering the bribe to Assistant Superintendent Chan Wai Hoong at Fortune Centre, Middle Road, on April 14, 2016.
Deputy Public Prosecutor Vincent Ong said ASP Chan and two others from the police force's compliance management unit were sent to Fortune Centre that day following a tip-off about unlicensed massage establishments and condoms in letterboxes.
The officers were walking around Level 4 of the building when they came across LK Beauty Spa, which purported to offer beauty and spa services. They noticed Zhu sitting inside. Once she saw the police officers, she closed the main door of the unit.
Sensing something amiss, the officers approached the unit to interview Zhu, who had previously worked there but was not managing it any more. Two bundles of keys were found in her handbag.
Suspecting that one of the keys was for the unit's letterbox, ASP Chan suggested that Zhu take the officers to the letterbox for an inspection.
Zhu began to panic and repeatedly pleaded with the officers not to open the letterbox. While walking to Level 1, Zhu continued to plead with ASP Chan not to open the letterbox and asked to settle the matter privately with him. ASP Chan ignored her.
At the letterbox, she admitted that the letterbox contained condoms and that these were used for providing sexual services at the unit. A black bag containing many unused condoms was seized.
A female staff sergeant was recording her statement when Zhu asked to speak to ASP Chan in private, but the latter refused and told her to say what she wanted in the other officer's presence.
Zhu then told ASP Chan in Mandarin: "I give you ten thousand dollars." He rejected the offer and warned her not to bribe a public servant. But Zhu said: "Ten thousand dollars you don't bring me back," intending to induce the officer to cease investigations as she was afraid that he would arrest her for providing sexual services.
He ignored her offer and asked his colleague to continue recording her statement.
Zhu was escorted to Police Cantonment Complex and arrested for suspected offences under the Women's Charter and bribery.
She has a conviction under the Massage Establishments Act.
Her lawyer Chung Ting Fai said the mother of two, who was from China but is now a Singapore citizen, deeply regrets her actions which have brought untold agony and trauma to her family. She could have been fined up to $100,000 and/or jailed for up to five years.


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• British police have arrested 12 men, aged 17-28, from London, Cardiff, and Stoke-on-Trent, said to be involved in a plot to bomb "unspecified targets" in the U.K., "inspired by Al Qaeda." This appears the largest arrest for terrorism in Britain since April 2009. [NYT]
• Terminal A at Newark Airport was shut down after a bag in a bag room tested positive for explosives. It turned out to be a computer monitor leaking a small amount of radiation. The terminal has reopened. [NBC NY, NYDN]
• Mayor Bloomberg is urging the Senate to pass the James Zadroga 9/11 Health Bill, which would provide medical coverage for 9/11 responders and survivors exposed to toxins from the attacks. On Thursday Senate Democrats failed to get the procedural vote to open debate on it, but yesterday they said they hoped to have changed the bill enough to get Republican support. The bill has passed in the House. [CNN]
• The NYPD is reviving their search to find the killer of 19-year-old Lissette Torres, found murdered on New Year's Day 1987 a mile from her home in Sunset Park. [NYP]
• Facebook CEO Mark Zuckerberg has been spotted in Beijing. He's said to have toured the Baidu complex with founder Robin Li and Zuckerberg's girlfriend Priscilla Chan. [Shanghaiist]
• Next Sunday is your last chance of the year to ride the MTA's Holiday Nostalgia train. Bowery Boogie did it! They have pictures.
This article from the Village Voice Archive was posted on December 20, 2010


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Gifts + Accessories
Sold Out $335.00 USD
Alexander Girard originally created the Wooden Dolls (1952), a whimsical assortment of figures both joyful and grim, for his own home. Today they add a charming touch to any interior.
Solid pine wood, hand-painted with mixed media, including feathers, twine, etc.
Large dog: 15.4" h | 4.3" w | 3.4" d
Each Doll comes individually boxed in wooden gift box. Made in Germany.
Call 760-230-1563
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Working with the body's Inner Physician at any level is a quantum technique. Anytime you merge, tune and drop your awareness into the tissue patterns of energy with intention you are engaging the body's wave function.
At these deep levels, your awareness opens into a realm where the Inner Physician begins to respond to you. Here is where intuitive information comes from. When you move and support merging into the wave function by mentally imaging, listening and ask for help, you are knocking on the door of transformation, that is when change happens.
The key to being neutral is, don't use your own energy nor agenda. When you do, tissue soreness and fatigue can occur, for both your client and yourself.
Stay in flow by following the energetic and tissue patterns, to locate and treat "Energy Cysts" – Dr, John Upledger, DO, OMM, with intention, and direction of energy – engage and ask for information at the quantum level, ask to be shown how to help. Then wait, be silent, inside the moment of the still point for an intuitive response.
Your intuition opens when you are truly listening, feeling and seeing – which happens when you are truly out of your own way and in neural. Pushing and Pulling can cause rebound and new injuries for both your client and your self.
At any time during the session you find yourself thinking, stop and tune back into the tissue patterns. Do this by simply moving your awareness back into it. You just don't want your monkey mind interrupting the session.
For more information about the Inner Physician www.Upledger.com
For more information call Claude at 214 232-5278 leave a voice or text message and I will get back with you as soon as possible.
© 2016 Claude Barrett, LMT, MTI, CST – All Rights Reserved.


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Trailers galore
I haven't really been working on anything terribly photogenic lately, hence the lack of blog posts for those thousands of you who have been waiting with bated breath.
My neighbor gave me a bunch of expired SCUBA tanks to play with. I cut them up into bells. They could also be used for a cool heavy-duty outdoor lamp, I imagine. There are maybe half a dozen left if you want one.
One of the tanks, cut into two bells/lamps.
Below is one of the two interchangeable tongues on a new trailer I have built for the Bike Collective. It's going to be a cool trailer. Pictures will be forthcoming when I get it back from the powdercoater.
The Collective's new trailer is going to be fully enclosed with this wooden tabletop as the lid. Here I'm putting a final coat of spar varnish on it to protect it from the inevitable rainfall. I don't like the high-gloss too much; I'm wondering if I can de-gloss it by sanding it with a really fine sandpaper. Wood finishing is not my specialty.
Here's a big utility trailer that I'm going to be working on next week:
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text: Makeup Monday: The Beauty Secret That Works From the Inside Out 11:57 AM PDT 8/24/2015 by Melissa Magsaysay FACEBOOK TWITTER EMAIL ME AP Images Gina Rodriguez - H 2015 HUM is the digestible beauty brand that's keeping Hollywood stars like Gina Rodriguez detoxed and blemish free. When it comes to perpetuating the latest beauty or wellness trend, Los Angeles arguably takes the (gluten-free) cake. Whether it's hot yoga, spinning, cleansing or colonics, it's a veritable buffet of health and well-being. Digestible beauty supplements are the latest aid adopted by celebrities and their hair and makeup artists, and an L.A. based brand called HUM provides a robust offering of skin, nail and body solutions. Read more You, Too, Can Dress Like 'Empire's' Cookie Lyon Molly Sims, Gina Rodriguez and Empire's Kaitlin Doubleday are fans of the Sephora-sold supplements, which are all targeted toward specific issues from bloating to blemishes. "The idea of HUM was born out of my personal struggle with breakouts," says Walter Faulstroh, co-founder of HUM. "I always knew that there was a link between nutrition and my skin but research in the area was hard to come by, with the majority of dermatologists not trained in the field of nutrition. It was only after I met with the most forward thinking nutritionists that I was able to fix my skin once and for all." Read more 'Empire's' Grace Gealey, Kaitlin Doubleday Talk Makeup Must-Haves Each gluten-free formula is created to tackle a certain issue. Ripped Rooster ($40) and Skinny Bird ($40) are weight loss supplements, Flawless + Fearless ($29) is meant to reduce breakouts and the celeb fave Red Carpet ($25) promises glowing skin and shiny, fuller hair in 6 weeks. Of course, it wouldn't be the latest health kick if it didn't come with a cleanse option. HUM has one called Cleanse to the Rescue ($42) , which entails 21 days of supplements said to result in radiant skin and a flatter stomach. Yes, please. "I'm a big fan of beauty supplements," says New York-based celebrity dermatologist Dr. Debra Jaliman. "Many of them contain antioxidants and biotin which helps give the skin glow and improve the nails and the thickness of the hair. I recommend that people take them twice a day. Best way to utilize the supplements is to eat a healthy diet as well and to avoid preservatives in your diet as well as packaged foods." See more See What All the Musicians Wore at Billboard's Hot 100 Music Fest No doubt that those who don't already, will be drinking down their beauty supplements with some green juice or kombucha. FACEBOOK TWITTER EMAIL ME Melissa Magsaysay email@example.com @thr


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Colorado beats Nebraska 65-51
Associated Press
Vail, CO Colorado
BOULDER, Colorado " He was trying to make a good final impression. Instead, Bill Callahan gave his athletic director and all those die-hard Nebraska fans one last glimpse of what a proud program looks like when it's disintegrating.
The once-mighty Cornhuskers gave up 34 straight points in the second half Friday en route to another loss by a videogame score " 65-51 to Colorado, which now has a shot at a bowl bid that could have gone to Nebraska.
Instead, the Huskers will go home with only their second losing record since 1962 " both have come during Callahan's four seasons in Lincoln.
Cody Hawkins threw for 241 yards and Hugh Charles ran for 169 yards and three scores to lift the Buffs (6-6, 4-4 Big 12) to the break-even mark in an up-and-down season that includes a victory over Oklahoma and a loss to Iowa State.
As always, a blowout over Nebraska (5-7, 2-6), in the highest-scoring game in the history of the 66-game series, will be a highlight for Colorado. But as everybody knows by now, this year's Huskers are nothing like the old Big Red.
Next up at Nebraska " that long-awaited meeting, scheduled for Saturday, between Callahan and interim athletic director Tom Osborne, the one-time coach who never would have let a debacle like this unfold at Folsom Field.
For a while, it didn't seem destined to end this way.
Nebraska rolled through the first half. The Cornhuskers scored 73 points in their last game, a win over Kansas State, and led 35-24 at halftime of this one after piling up 393 yards against a Buffs defense that has had plenty of its own problems this year.
In the third quarter, some poor sideline management turned the game into another Big Red embarrassment.
Leading by 11 and facing third-and-2 from its own 19, Nebraska got nailed with consecutive 5-yard penalties " once for illegal substitution and once for delay of game. That made it third-and-12, and on that play, Huskers quarterback Joe Ganz threw the ball straight to Colorado cornerback Jimmy Smith, who returned it 31 yards for a touchdown.
On Nebraska's next offensive play, Ganz underthrew Nate Swift, who batted the ball in the air to be intercepted by Lionel Harris. Four plays later, Charles ran 7 yards to put Colorado ahead 44-35.
Nebraska's next drive ended in a blocked punt, which led to another CU touchdown, and suddenly a 10-point Nebraska lead had become a 16-point deficit.
Ganz threw another pick late in the fourth quarter " this one batted up by receiver Maurice Purify, which led to a 51-yard interception return by Cha'pelle Brown and Charles' third score for a 58-35 lead.
That was 34 straight points, five straight touchdowns, and when the Buffs answered a garbage Nebraska touchdown with one of their own, they because the second team to hang 60 on the Huskers this month. Kansas beat Nebraska 76-39 three weeks ago.
For Colorado, this win will look good alongside that 62-36 blowout over Nebraska in 2001 that led to CU's last Big 12 title. This was the first time CU has cracked 60 since then.
Ganz finished with 484 yards passing for the Huskers and holds plenty of promise for whoever coaches him next year. Purify had 11 catches for 136 yards and three scores, but also had his share of drops and bobbles that cost the Huskers.
Of course, offense hasn't been Nebraska's problem this year.
The defense surrendered 518 yards, which was 45 yards above its average.
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One of the hundreds of posts in my feed-reader this morning was about the British Library electronic theses service (via SCIT blog). As my own thesis should be included I decided to indulge in a bit of vanity searching. Result: EThOS has a long way to go.
I would expect my thesis to turn up for the term 'webometrics', in fact it is about the only term for which someone might actually want to read it. Unfortunately the only webometric thesis belongs to Xuemei Li:
My thesis does however turn up for the wholly inappropriate 'bibliometrics':
Seemingly the reason for my appearance under 'bibliometrics' and not 'webometrics' is that 'bibliometrics' appears in my abstract whereas 'webometrics' does not. Whilst this may seem reasonable at first, theorectically the University of Wolverhampton are taking part in the project and their record includes a number of keywords carefully selected me, including 'webometrics'. The British Library also fails to provide a link to my thesis, despite it being scattered over the web like confetti: "Not yet available for download".
Young academics brought up on Google Scholar, with full text searching and links to the numerous copies on the web, are unlikely to see the value in EThOS and its traditional OPAC style. Whilst I'd like to see an electronic thesis online service that seperates the wheat from the chaff, with full text searching and links to the documents, and believe that librarians could aid in retrieval with classification of such documents, this is not what EThOS is currently offering. It's still in Beta, and likely to improve, but it has a frighteningly long way to go and you do wonder whether they should have buddied up with one of the big search engines to produce a more user friendly version.


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Courtesy of Banana Books in Long Beach, Washington
After reading about the horrors of the Holocaust in The Search for Major Plagge, I had to switch to something cheerful. Enter the charming wackiness of ichthyologists in hot pursuit of their passions in A Fish Caught in Time: The Search for the Coelacanth. Ironically, this book also starts in 1938.
Just as Europe was bracing for what would become WWII, in the Indian Ocean off the coast of South Africa, a fossil fish was going about its business, neither knowing or caring that the human world "knew" it to be extinct.
The Lady Curator
Marjorie Courtenay-Latimer was the young head of an obscure museum in East London, South Africa. Like animal advocate and zookeeper Gerald Durrell, she collected everything she could get her hands on, including fish. She had an agreement with the captain of a trawler, a commercial fishing boat, that if he caught anything unusual, he'd let her know and she'd identify it for her museum.
Three days before Christmas in 1938, as Gone With the Wind was opening in America, Latimer went down to the docks to look at a pretty, blue, 5-foot long fish she'd never seen before. It had four fins like arms and legs. It weighed 127 pounds. She knew immediately it was rare and possibly precious, and she had to get an expert in to look at it. That meant she had to preserve the fish despite the tropical heat. But how? No ordinary family had a refrigerator. She thought of the morgue, but they adamantly refused to take on "a stinking fish". She tried the town's "cold storage", but they also refused, saying that the fish could give off gasses, and might contaminate the food. She was out of options. Reluctantly, she took the fish to the town's sole taxidermist and had him stuff it.
Then she contacted South Africa's preeminent ichthyologist, J.L.B. Smith, on holiday several hundred miles away. Upon arriving in East London, he was able to identify the fish as a coelacanth–a fossil fish with a bony exoskeleton–a fierce predator thought to be as extinct as the T-Rex, as dead as the Dodo. Coelacanths are thought to have been around for 75 million years.
Old J.L.B. nearly wet his pants with excitement on seeing this fish. For the next 14 years, he tried to find another one. First, the outbreak of World War II put a stop to his plans. (He was exempt from service due to his WWI injuries, but commercial fishing came to a dead halt and belligerent ships plied the waters). After that he tried offering a 100 British pound reward for another coelacanth with its insides intact. He and his wife tried looking for the fish's habitat themselves, but with no luck.
Until one was hauled in by a native fisherman in the Comoros Islands. And another race against time began…Again, it was just before Christmas. Again, he was hundreds of miles away–this time in another country. Could he beat the French (in charge of the Comoros) to the prize?
An Adventure in the Spirit of Indiana Jones
Or perhaps Clive Cussler. This is no dry, dusty tome about a boring subject–fish. It has controversy, drama, and eccentric characters galore. The controversy comes in because of Charles Darwin's theories of evolution, which had gained exactly no purchase in South Africa. The government refused to allow anything from The Origin of Species to be taught in schools (right up until the 1990s, in fact.) And unfortunately, this fish looked a lot like a transition fossil–the "Missing Link" that would show how fish crawled out of the oceans to sprout legs and lungs and become reptiles, apes, and then humans. (Or as we said back then–Man.)
The Smiths received a lot of letters from religious fundamentalists, which J.L.B. kept in what he called "the crackpot file".
Despite knowing that I probably would have disliked J.L.B. Smith intensely in real life, like many eccentrics he is delightful to read about. A socially awkward genius who didn't bother with the niceties, Smith married a much younger woman. I don't think his ego could have tolerated a strong female his own age. Described as a slight man with a fragile body, I read between the lines that he probably had a Napoleon complex.
But his ill health too was interesting. At the outbreak of WWII, J.L.B. was given 5 years to live. He immediately decided to defy his doctors by going for long walks every day of at least 4 miles or more, and by changing his diet. He also refused to ever wear closed-toe shoes.
The Diet Debacle
As a person with gluten intolerance and diabetes, I wanted to know more about this life-saving diet. Unfortunately author Weinberg let me down.
"He refused to mix his proteins and carbohydrates," she writes. (Why? When you eat carbs without proteins, your sugar shoots straight up and then straight back down. Combining carbs with proteins as my doctors advise, rounds out the curve, making it gradual and gentle.)
"He never ate meat with vegetables, or bread with butter and cheese." (Um…meat is a protein but vegetables are not carbs, unless you mean peas/carrots/corn.)
"People thought he was crazy. His sandwiches, remembers Jean Pote, used to consist of two pieces of cheese with some apple wedged between them. (Hello, apples are carbs! Fruit has fructose–a fruit sugar–a carbohydrate, and LOTS of it.)
This inaccurate reporting made me wonder what else the author had gotten wrong. How far could I trust her? I kept reading, but with a shaker of salt, occasionally fact-checking for myself on the Internet.
Ironically, old J.L.B. Smith got some things wrong himself, including the name of the black man who actually caught the second coelacanth. I won't give that name here, but the fisherman's real name was Ahamadi Abdallah. Smith also thought for a while that this second fish was a different species of coelacanth, before realizing that the dorsal fin and tail were only different from the first fish due to a shark attack.
Fun Fact: Grande Comoro, the largest of these islands, boasts the mountain of Karthala, the largest active volcano crater in the world.
Rating: Five Kapok trees!
You can find my blog about a book set in South Africa here.


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On Monday. I took my final pregnancy test.
"We're required to do one whenever we are doing surgery", the nurse explained.
The irony of a pregnancy test prior to a hysterectomy was not lost on me.
"It just goes to show," my friend Mombie texted, "There have been enough malpractices for performing hysterectomies on pregnant patients."
Other than the 10cm dermoid that has attached itself to my left ovary (which I have nicknamed my alien baby) – pregnant is one of those things I have never been.
I remember buying pregnancy tests when I thought my husband and I would try for a baby. The box sat on the shelf haunting me as months went by and nothing worth testing was happening. I remember my less-than-joking conversations about him just putting some in a cup and I would use a turkey baster.
I remember discussing infertility options with my friends in couples class through our church. Our small group soon got divided as we became the haves and the have nots. Those of us who were childless started to feel like the kids who got held back and it was hard to deal with the depression of those around me who were trying so hard and yet were left without.
I knew what my problem was, as my marriage was rocky and lacked the intimacy required to even get the process started. But my heart ached for my other friends.
We looked briefly at becoming foster parents, but I realized in my heart that my husband would not be the kind of father I would want for any child – he had too many anger issues to provide a safe and stable environment. (It took me several years to realize that if the situation was not mentally healthy for a child, it was probably not healthy for me either, but I did wise up eventually.)
After my marriage ended, getting pregnant meant something different. I remember the first time that I thought I might be pregnant – the rush of emotions as I tried to figure out what I would do if I was. I had just started rebuilding my life, and the thought of being responsible for another being scared me so much.
I don't know what it feels like to have a line turn blue, or show a plus sign, or flash "pregnant" and now I never will. I wouldn't say I'm sad, nor would I say I'm euphoric. I'm more thoughtfully respectful that an aspect of possibility is now being laid to rest.


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Tuberculosis (TB) is an infectious disease caused mainly by the bacillus Mycobacterium tuberculosis, and remains one of the world's deadliest contagious diseases. TB incidence is slowly declining each year, however, novel drugs and vaccines are urgently needed to stop global transmission and to prevent the development of drug-resistant strains (WHO 2016). The better understanding of important metabolic pathways from M. tuberculosis can contribute to the development of novel therapeutic and prophylactic strategies to combat TB. Purine nucleotides in M. tuberculosis may be formed de novo from simple precursors, or may be obtained by the salvage pathway from preformed purine bases and nucleosides. While the de novo pathway is a high energy demanding process, which involves up to 11 enzymatic steps, the salvage pathway might be the main source to maintain the nucleotide pool under conditions of low energy availability or rapid multiplication (Ducati et al. 2011). Nucleoside hydrolase (MtIAGU-NH), encoded by iunH gene (Rv3393, Gene ID: 887625), is an important enzyme from the purine salvage pathway in M. tuberculosis, which catalyses the irreversible hydrolysis of N-glycosidic bond of ribonucleosides forming a-D-ribose and the corresponding base (Wink et al. 2013). MtIAGU-NH was shown to have broad substrate specificity, accepting inosine, adenosine, guanosine, and uridine as substrates, which may point to a pivotal metabolic role in M. tuberculosis (Wink et al. 2013). Here, we describe the construction of a M. tuberculosis knockout (KO) strain for iunH gene, in vitro growth studies, and the effect of iunH deletion in M. tuberculosis in non-activated and activated macrophage model of infection, comparing with M. tuberculosis H37Rv wild-type (WT) and complemented (CP) strains.
Plasmid construction for generation of the knockout strain - A fragment of 1782 bp containing the iunH gene (927 bp) with its flanking region (Fig. 1A) was amplified by polymerase chain reaction (PCR) from M. tuberculosis H37Rv genomic DNA, using primers forward (5'-tttttctagagcagcaggcgatgcgccagg-3') and reverse (5'- tttttctagagacccgtcgccggcggtgc-3'), both containing XbaI restriction sites (underlined). The 1782 bp fragment was subsequently cloned into pUC19 using the XbaI restriction site. The iunH gene was disrupted by the insertion of a kanamycin cassette from pUC4K into unique internal enzyme restriction site XcmI (New England Biolabs, USA) (Fig. 1B). Insert was released from pUC19 derivative vector by digestion with XbaI (New England Biolabs, USA), and subcloned into XbaI linearised pPR27xylE vector (pPR27xylE::iunH kan) (Fig. 1B) (Pelicic et al. 1997).
Plasmid construction for generation of the complemented strain - The iunH gene flanked by about 200 bp upstream and 100 bp downstream, was amplified by PCR from M. tuberculosis H37Rv genomic DNA using primers forward (5'-ttttctagacagcgcgagatcgatcttg-3') and reverse (5'-tttttctagacggtggtatctggagggaa-3'), both containing XbaI restriction sites (underlined), and was cloned into XbaI linearised pNIP40/b (pNIP40::iunH), a mycobacteriophage Ms6-derived integrative vector (Freitas-Vieira et al. 1998).
Construction of the M. tuberculosis knockout strain - Electrocompetent cells were prepared as described (Parish & Stocker 1998) with some modifications. M. tuberculosis H37Rv strain was grown in 50 mL of Middlebrook 7H9 (Becton Dickinson, BD, USA) 10% OADC (oleic acid, albumin, dextrose, and catalase) (BD, USA) 0.05% tween-80 (Sigma-Aldrich, USA) (liquid medium) to an OD600 of 0.6. Cells were washed two times in 0.05% tween-80, one time in 10% glycerol containing 0.05% tween-80, and were suspended in 500 µL of 10% glycerol containing 0.05% tween-80. Aliquots (200 µL) of fresh prepared competent cells were electroporated with approximately 2 µg of pPR27xylE::iunH kan plasmid in 0.2 cm cuvettes with a single pulse (2.5 kV; 25 mF; 1000 ohms). The pPR27xylE plasmid contains a thermosensitive origin of replication, the xylE reporter gene, and the sacB counterselectable marker. Bacteria were plated on Middlebrook 7H10 (BD, USA) 10% OADC (solid medium) containing 25 µg/mL kanamycin (Gibco, USA), and incubated at 32ºC. After six weeks, 1% pyrocatechol (Sigma-Aldrich, USA) was dropped on colonies to select those containing the plasmid. Three different yellow colonies were picked up from the transformant, and cultivated in liquid medium containing kanamycin 25 µg/mL at 32ºC. Individual cultures were plated on solid medium containing 25 µg/mL kanamycin, 2% sucrose (Fisher Scientific, USA), and cultivated at 39ºC. After four weeks, 1% pyrocatechol was dropped on colonies to select those that might be double crossover (DCO) strains. Nine white colonies were inoculated in liquid medium containing 25 µg/mL kanamycin, and cultivated at 37ºC for three weeks. Genomic DNA was isolated and PCRs were carried out using gene-specific screening primers forward (5'- ttcaggaaacgagcgaaggt-3') and reverse (5'-gtgctatccggcggacac-3') to determine whether the WT or the KO strain was present in the targeted chromosomal region (Fig. 1B).
Construction of the M. tuberculosis complemented strain - In order to obtain the CP strain with the iunH gene, the KO strain was transformed by electroporation with about 2 µg of the complementing plasmid construction, pNIP40::iunH. Electrocompetent cells were prepared as described above. Bacteria were plated on solid medium containing 50 µg/mL hygromycin (Invitrogen, USA) and 25 µg/mL kanamycin, and incubated at 37ºC. After three weeks, a single colony was cultivated in 5 mL of liquid medium with 50 µg/mL hygromycin 25 µg/mL kanamycin at 37ºC.
Protein extraction - WT, KO and CP strains were grown in 50 mL liquid medium containing the proper antibiotics until an optical density at 600 nm (OD600) of 0.5-0.7. Cellular pellets were washed twice using 10 mM Tris HCl pH 8.0. Cells were resuspended in 1 mL of the same buffer containing protease inhibitor cocktail (Promega, USA), and then transferred to 2 mL lysing matrix B tubes containing 0.1 mm diameter silica beads (MP Biomedicals, USA). Cells were disrupted using a L-Beader 3 (Loccus, Brazil) at a speed setting of 4000 rpm, 10 cycles of 30 s each, cooling between cycles. After lysis, the cell free supernatants were collected by centrifugation at 2300 x g for 10 min at room temperature. The supernatants were filtered through 0.22 mM Millex Durapore (Millipore, USA), and triton X-114 (Sigma-Aldrich, USA) extraction was carried out to obtain detergent and aqueous fractions as described previously (Malen et al. 2010).
Western blot - Anti-MtIAGU-NH mouse polyclonal antibody was produced by immunising a mouse with 50 mg of MtIAGU-NH purified protein (Wink et al. 2013) containing Freund's incomplete adjuvant (Sigma-Aldrich, USA) (total volume of 100 µL) by subcutaneous route, followed by a booster injection after one month. After one more month, mouse was euthanised by deep isoflurane inhalation, and blood was collected by the descendant aorta. Serum was separated by centrifugation at 10,000 x g, for 10 min, aliquoted, and storage at -80ºC. Mycobacterial proteins from detergent fraction (50 mg) were loaded on sodium dodecyl sulphate 12% polyacrylamide gels (SDS-PAGE), and transferred to nitrocellulose membranes (iBlot Invitrogen, USA). Blots were blocked with 5% non-fat dried milk (Santa Cruz Biotechnology, USA) 0.05% tween-20 (Sigma-Aldrich, USA) in TBS (T-TBS), and probed with anti-MtIAGU-NH polyclonal mouse antibody in a 1:500 dilution. Membranes were washed three times with T-TBS, and alkaline phosphatase-conjugated anti-mouse secondary antibody (Invitrogen, USA) was used at a dilution of 1:5000. Chemiluninescent substrate (Novex by Life Technologies, USA) was used for detection with ChemiDoc (Bio-Rad, USA).
LC-MS/MS - The presence of MtIAGU-NH in the WT, KO and CP strains was also investigated by liquid chromatography coupled to mass spectrometry (LC-MS/MS) of SDS-PAGE slices. Sections from 28-40 kDa of each lane/strain were excised and submitted to in-gel digestion (Shevchenko et al. 2006). LC-MS/MS of peptides was performed on an Eksigent nanoLC Ultra 1D plus with AS-2 autosampler, coupled to a LTQ-XL Orbitrap Discovery (Thermo Scientific, USA). Peptide mixtures from SDS-PAGE slices were separated in reversed phase gradients in C18 (5 µM ODS-AQ C18 Yamamura Chemical Lab) column. Samples were analysed in technical triplicates. Mass spectra were acquired in a top 8 data-dependent manner with dynamic exclusion applied, and were searched against a non-redundant M. tuberculosis database for candidate peptides with the software Comet (Eng et al. 2012) in the platform PatternLab for Proteomics (Carvalho et al. 2016). The validity of the peptide spectra matches was assessed using the module Search Engine Processor from Patternlab for Proteomics, with a false discovery rate of 1%. Identified peptides from MtIAGU-NH were manually validated and peak areas of selected peptides were calculated with Skyline (MacLean et al. 2010).
In vitro characterisation - Growth curves were determined in Sauton's medium containing 0.025% tyloxapol (Sigma-Aldrich, USA), in duplicates, at 37ºC, 80 rpm until reach the stationary phase. Aliquots were removed from each culture at different time points and the OD600 was determined. The data were evaluated with the two-way ANOVA analysis, followed by Bonferroni's post-test, using GraphPad Prism 5.0. Differences were considered significant at the 95% level of confidence. In order to quantify the intracellular concentration of the bases (uracil, guanine, adenine, and hypoxanthine) and nucleosides (uridine, guanosine, adenosine, and inosine) in the WT, KO and CP strains, bacteria were grown in Sauton's medium until an OD600 of 0.6 - 1.0. Cells were washed twice with 1x PBS, and autoclaved at 121ºC for 30 min, followed by sonication. An HPLC method (Dionex Ultimate 3000, Thermo Scientific, USA) to identify and quantify bases and nucleosides was modified from Wink et al. (2013). Solution A (1% acetic acid) was maintained at 100% for 20 min, followed by a linear gradient of 2 min up to 80% B (20 mM ammonium acetate) and 20% C (methanol and acetonitrile 1:1, v/v) for 10 min. The absorbance was measured at 262 nm, injection volume was 50 µL, 0.5 mL/min, and measurements were performed in triplicates.
Macrophage infection - Macrophage infection experiments are often used to determine mycobacterial strains virulence (Copenhaver et al. 2004, Katti et al. 2008). RAW 264.7 macrophage cell line was cultured in DMEM (Gibco, USA), supplemented with 10% heat inactivated fetal bovine serum (FBS) and 1% penicillin-streptomycin at 37ºC with 5% CO2. Before infection procedures, macrophages were seeded in 24-well culture plates at a density of 105 cells per well in DMEM medium with 10% FBS and incubated for 24 h at 37ºC with 5% CO2. Two independent experiments were performed, one with non-activated and the other with activated macrophages, which were activated by the addition of 5 ng/mL of interferon-g (IFN-g) (R&D systems, USA) during 24 h. Infection of RAW 264.7 cells with WT, KO and CP strains was performed at a multiplicity of infection of 2:1 (bacteria/macrophage) at 37ºC with 5% CO2. After 18 h, infection was terminated by removing the overlaying medium, and each well was washed twice with sterile 0.9% NaCl solution to remove extracellular bacteria. At 18 h after infection, and two, three, six, seven and 10 days of incubation, wells were washed with sterile 0.9% NaCl solution, and the infected macrophages were then lysed with 0.025% SDS (Rodrigues Jr et al. 2014). DMEM media was changed after every three days of incubation. Lysates were serially diluted and plated on solid medium. Bacterial colony formation was evaluated after incubation of plates for three weeks at 37ºC. These experiments were performed in triplicates of each time point. The results were expressed as mean numbers of the logarithms of CFU per well, and were evaluated with the two-way ANOVA analysis, followed by Bonferroni's post-test, using GraphPad Prism 5.0. Differences were considered significant at the 95% level of confidence.
Construction of the M. tuberculosis knockout and complemented strains - Among the nine clones screened for the KO of iunH gene, all suffered a double-crossover gene replacement event (Fig. 1C). To construct the CP strain, the pNIP40::iunH construction was transformed in the KO strain. The stability of the mutation introduced by gene replacement in M. tuberculosis was evaluated by plating KO and CP strains on media with and without antibiotics. The difference between the colonies obtained on plates containing antibiotics was not significant when compared with the ones obtained on plates without antibiotic, which indicates that the introduced mutation is stable (data not shown).
Evaluation of iunH knockout at protein level - As shown in Fig. 2A, a 32.9 kDa band was detected by Western blot assay in extracts from WT and CP strains but was absent in the KO strain. The identity of the protein was further confirmed by LC-MS/MS from SDS-PAGE slices (28-40 kDa). Spectra matching MtIAGU-NH peptides were identified in WT and CP but not in KO extracts (Fig. 2B), confirming that the protein observed in WT and CP extracts by western blot is in fact MtIAGU-NH. As shown in Fig. 2B, the areas of the peptides identified on the WT sample are approximately 3-fold higher than the areas identified on CP sample. No peptides were identified on the KO sample; consequently peak areas could not be calculated. Fig. 2C shows a representative MS/MS spectrum of the peptide LASVCGSSPVMR, identified in both WT and CP samples. These results indicated that the disruption of iunH gene abolishes the expression of MtIAGU-NH protein in KO strain, and the MtIAGU-NH protein expression was restored in CP strain.
In vitro characterisation - The growth rate of the WT, KO, and CP strains were compared to determine whether iunH disruption lead to alterations during in vitro cultivation. Sauton's is a defined medium that was used for in vitro characterisation experiments to avoid interferences with traces of bases and nucleosides from 7H9 medium. As shown in Fig. 3A, the deletion of iunH gene led to a delay of growth in Sauton's medium during log phase. The iunH KO strain entered the early log phase of growth (OD600 of 0.2) six days later than WT strain (inset in Fig. 3A). No significant differences were found during lag and stationary phases of growth between WT and KO strains (Fig. 3A). Two independent experiments were performed, and similar results were obtained. The intracellular concentration of the bases and nucleosides was quantified in the WT, KO and CP strains. Uracil, guanine, adenine, hypoxanthine, uridine, inosine, guanosine and adenosine presented retention times of 10.53, 13.35, 14.34, 16.98, 19.89, 29.51, 29.64, and 30.36 minutes respectively. The calibration curves for all analyses were made from 0.48 to 62.5 µM and all presented a correlation coefficient above 0.99. No significant difference was observed in intracellular concentrations of either bases or nucleosides among the three strains when grown in Sauton's medium (Fig. 3B). The absence of accumulation of purine bases and nucleosides in iunH KO strain could be explained by the redundancy found in nucleotide metabolism of M. tuberculosis. There are at least five enzymes in nucleotide salvage pathway that use MtIAGU-NH substrates or products: purine nucleoside phosphorylase (Rv3307), hypoxanthine-guanine phosphoribosyltransferase (Rv3624c), adenine phosphoribosyltransferase (Rv2584c), uracil phosphoribosyltransferase (Rv3309c), and pyrimidine nucleoside phosphorylase (Rv3314c) (Ducati et al. 2011, Villela et al. 2011); which might compensate for the absence of iunH gene in KO strain.
Macrophage infection - To examine whether the iunH gene was important for invasion and growth in phagocytic cells, we determined the bacterial loads of the WT, KO and CP strains by using the macrophage model of infection. Non-activated macrophages were infected with 1.4 x 105, 2.2 x 105, and 2.0 x 105 CFU, while activated cells were infected with 2.6 x 105, 2.5 x 105, and 2.6 x 105 CFU of WT, KO and CP strains, respectively, as determined at the day of infection. As shown in Fig. 4A, no significant difference was observed in intracellular growth among WT, KO, and CP strains in non-activated macrophages after 18 h, two, three, seven and 10 days after infection. Similar results were obtained with IFN-g activated macrophages, where no significant difference in bacterial load of KO strain was observed when compared with both WT and CP strains after 18 h, two, three and six days after infection (Fig. 4B). The disruption of iunH gene does not affect the M. tuberculosis growth in both non-activated and IFN-g activated RAW 264.7 cells. The concentration of 5 ng/mL of IFN-g or lower was shown to cause the activation of the endocytic pathway during the immune activation of RAW 264.7 (Pei et al. 2015), to induce the upregulation of the pro-inflammatory cytokine IL-18 in RAW 264.7 mouse macrophages (Kim et al. 2000), and to lead to its own production in mouse peritoneal macrophages (Di Marzio et al. 1994). In order to evaluate cytokine expression by non-activated macrophages infected with WT, KO and CP strains, the supernatants from 18 h, and three days post infection were collected and interleukin (IL)-1b, tumor necrosis factor (TNF)-a, and IFN-g were quantified by ELISA using a commercial kit (data not shown). TNF-a and IL-1b levels of expression were not significantly different among groups, while IFN-g expression was not detected in culture medium of cells infected with all strains (data not shown).
In this work, we constructed a M. tuberculosis KO strain for iunH gene, validated the gene deletion at protein level, characterised the KO strain in vitro, and evaluated its ability to invade and grow in non-activated and IFN-g activated macrophages. The absence of accumulation of purine bases and nucleosides in M. tuberculosis iunH KO strain in Sauton's medium, together with the fact that iunH gene is not important for M. tuberculosis virulence in macrophages, could be explained by the redundancy found in nucleoside/nucleotide metabolism of M. tuberculosis. As mentioned previously, there are at least five enzymes in nucleotide salvage pathway from M. tuberculosis that use MtIAGU-NH substrates or products (Ducati et al. 2011, Villela et al. 2011). These enzymes might compensate for the absence of iunH gene in KO strain, consequently maintaining the nucleotide pool within the cell. Our results indicated that MtIAGU-NH is not a target for drug development.
ADV constructed and validated the Mycobacterium tuberculosis knockout and complemented strains and wrote the manuscript; ADV and VSRJ performed macrophage infection experiments; AFMP carried out LC-MS/MS experiments; PLW and ZASQ constructed the plasmids; ADV and GOP performed in vitro characterisation; MMC participated in the design of the study and statistical analysis; LAB contributed in the analysis of results and revised the manuscript; and DSS conceived the study and participated in its design and coordination.
To Dr Mary Jackson and Dr Brigitte Gicquel, for providing the pNIP40/b and pPR27xylE plasmids.
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Villela AD, Sánchez-Quitian ZA, Ducati RG, Santos DS, Basso LA. Pyrimidine salvage pathway in Mycobacterium tuberculosis. Curr Med Chem. 2011; 18(9): 1286-98.
WHO - World Health Organization. Global tuberculosis report 2016. Geneva: WHO; 2016.
Wink PL, Sánchez-Quitian ZA, Rosado LA, Rodrigues Jr VS, Petersen GO, Lorenzini DM, et al. Biochemical characterization of recombinant nucleoside hydrolase from Mycobacterium tuberculosis H37Rv. Arch Biochem Biophys. 2013; 538(2): 80-94.


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PracticePanther's Law Office Software API
The PracticePanther API gives you access to all the features you see in our app and lets you extend them for use in your own app. It strives to be RESTful, supports OData Queries, and is organized around the main resources you're familiar with from the PracticePanther web interface.
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In the stream of excellent appointments coming from Trump Tower, Donald's naming of Iowa governor Terry Branstad as Ambassador to China hasn't gotten a lot of notice, apart from the obvious point that farmers are the biggest free traders. That is true, of course, but a very smart reader with deep knowledge of international trade suggests that there is more to the Branstad appointment:
Trump could well be making a very shrewd appointment here. Terry Branstad has credibility both in the U.S. and China, not to mention influence over upwards of 25 electoral votes! He clearly has deep connections with China. But the real significance of the pick may be the exact opposite of what seems to be the case, if Trump's trade proposals, essentially a threat of trade war, are taken at face value.
I think Terry Branstad is acutely aware of how important the China export market is — for Big Ag. Soybeans alone are enormous, the third largest global commodities market, if I am not mistaken, after oil and coffee. We are in huge competition with Brazil, which is neck-and-neck with the U.S. in domination of the global soybean market, of which China is the largest customer. In addition ag related manufacturing is big in Iowa and elsewhere in the Midwest; firms like Caterpillar and John Deere (HQ in Iowa [UPDATE: Rather, across the river in Moline, Ill.]) compete with global firms for business all over the world, including China. There is no way the Trump voters in Des Moines, Dubuque, Decatur and Dayton want a trade war with China.
So what's going on? Forget about the largely bogus accusations of dumping, export subsidies and, especially currency manipulation. The Chinese currency has had a long term trend of increasing value and not export-motivated devaluation, as noted by the IMF and the U.S. Treasury Department. Furthermore, China's entry into the WTO in 2005 had a positive impact on U.S. exports:
China's entry into the WTO did not require the United States to lower its duties on Chinese imports, nor did it relax rules against Chinese subsidies. The main impact was to reduce Chinese barriers to exports from the United States. And there China's entry has had measurable, positive impact.
The Office of the U.S. Trade Representative elaborated:
The annual tariff changes that China made following its WTO accession significantly increased market access for U.S. exporters in a range of industries, as China reduced tariffs on goods of greatest importance to U.S. industry from a base average of 25 percent (in 1997) to approximately 7 percent, while it made similar reductions throughout the agricultural sector. [emphasis added]
No doubt Terry Branstad is well aware of these facts and very sensitive to both the importance of U.S. ag related exports and the vulnerability of the ag sector to trade retaliation. In fact, the biggest issue for U.S. exports is the lingering Chinese tariffs and other exclusions on certain ag products from the U.S., arising from prior trade disputes in other sectors.
If Branstad can assuage the tensions in U.S. – China trade relations, and increase primarily ag exports by reducing existing Chinese trade barriers, it would tend to reduce the U.S. trade deficit, perhaps substantially. This achievement, for what it's worth, might be possible without resorting to a ruinous trade war, punitive tariffs to protect low-value added manufacturing at the expense of more important sectors, or the rhetoric of demagogic and exaggerated claims and threats. Trump would be able to claim victory and go home, while pretending that his bluster forced concessions from China and gave us "better deals." Such a political victory based on ag exports reducing the trade deficit, while ignoring the false promise to "bring back our stolen [manufacturing] jobs", would be quite good enough for government work. Trump would demonstrate again that he is not to be underestimated politically.


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Dèjatch Alámayou / King Theodore's Son
- Object:
- Place of origin:
Isle of Wight (photographed)
- Date:
July 1868 (photographed)
- Artist/Maker:
Cameron, Julia Margaret, born 1815 - died 1879 (photographer)
- Materials and Techniques:
Albumen print from wet collodion glass negative
- Credit Line:
Given by Mrs Ida S. Perrin, 1939
- Museum number:
- Gallery location:
Prints & Drawings Study Room, level F, shelf X, box 311V
When Julia Margaret Cameron photographed her intellectual heroes such as Alfred Tennyson, John Herschel and HenryTaylor, her aim was to record 'the greatness of the inner as well as the features of the outer man.' Another motive was to earn money from prints of the photographs, since her family's finances were precarious. Within her first year as a photographer she began exhibiting and selling through the London gallery Colnaghi's. She used autographs to increase the value of some portraits.
Prince Dèjatch Alamayou was the son of an Ethiopian emperor who committed suicide rather than surrender to the British in 1868. The orphan prince came to live on the Isle of Wight, where Queen Victoria took a particular interest in him, paying for his education and allowing him to be buried at Windsor Castle when he died of pleurisy aged eighteen.
Cameron took this photograph of Alámayou during one of his visits to Queen Victoria at Osborne House, Isle of Wight, in 1868. Probably responding to public interest in the orphan African prince, Cameron has presented her subject as a melancholy and romantic figure. The photograph was initially printed in large format but was reproduced in the form of cabinet cards and cartes de visite.
Physical description
Photograph of a seven-year-old boy (Prince Alámayou), seated, wearing a white shirt and necklace with large pear-shaped pendants. A shield has been positioned behind the child who looks off to his left whilst holding up a doll on a toy couch. The same shield appears in a Cameron photograph of Captain Speedy (19-1939).
Place of Origin
Isle of Wight (photographed)
July 1868 (photographed)
Cameron, Julia Margaret, born 1815 - died 1879 (photographer)
Materials and Techniques
Albumen print from wet collodion glass negative
Marks and inscriptions
Below photograph, on left: 'From Life Registered Photograph July 1868', on right: 'Julia Margaret Cameron'. Below, centre: 'Dejátch Álámáyou' / [Text in Amharic?] / King Theodore's Son'.
Height: 41 cm (mount), Width: 32.5 cm (mount), Height: 29.5 cm (photograph), Width: 23.5 cm photograph
Object history note
Julia Margaret Cameron (1815–79) was one of the most important and innovative photographers of the 19th century. Her photographs were rule-breaking: purposely out of focus, and often including scratches, smudges and other traces of the artist's process. Best known for her powerful portraits, she also posed her sitters – friends, family and servants – as characters from biblical, historical or allegorical stories.
Born in Calcutta on 11 June 1815, the fourth of seven sisters, her father was an East India Company official and her mother descended from French aristocracy. Educated mainly in France, Cameron returned to India in 1834.
In 1842, the British astronomer Sir John Herschel (1792 – 1871) introduced Cameron to photography, sending her examples of the new invention. They had met in 1836 while Cameron was convalescing from an illness in the Cape of Good Hope, South Africa. He remained a life-long friend and correspondent on technical photographic matters. That same year she met Charles Hay Cameron (1795–1880), 20 years her senior, a reformer of Indian law and education. They married in Calcutta in 1838 and she became a prominent hostess in colonial society. A decade later, the Camerons moved to England. By then they had four children; two more were born in England. Several of Cameron's sisters were already living there, and had established literary, artistic and social connections. The Camerons eventually settled in Freshwater, on the Isle of Wight.
At the age of 48 Cameron received a camera as a gift from her daughter and son-in-law. It was accompanied by the words, 'It may amuse you, Mother, to try to photograph during your solitude at Freshwater.' Cameron had compiled albums and even printed photographs before, but her work as a photographer now began in earnest.
The Camerons lived at Freshwater until 1875, when they moved to Ceylon (now Sri Lanka) where Charles Cameron had purchased coffee and rubber plantations, managed under difficult agricultural and financial conditions by three of their sons. Cameron continued her photographic practice at her new home yet her output decreased significantly and only a small body of photographs from this time remains. After moving to Ceylon the Camerons made only one more visit to England in May 1878. Julia Margaret Cameron died after a brief illness in Ceylon in 1879.
Cameron's relationship with the Victoria and Albert Museum dates to the earliest years of her photographic career. The first museum exhibition of Cameron's work was held in 1865 at the South Kensington Museum, London (now the V&A). The South Kensington Museum was not only the sole museum to exhibit Cameron's work in her lifetime, but also the institution that collected her photographs most extensively in her day. In 1868 the Museum gave Cameron the use of two rooms as a portrait studio, perhaps qualifying her as its first artist-in-residence. Today the V&A's Cameron collection includes photographs acquired directly from the artist, others collected later from various sources, and five letters from Cameron to Sir Henry Cole (1808–82), the Museum's founding director and an early supporter of photography.
Descriptive line
Photograph by Julia Margaret Cameron, 'Dèjatch Alámayou / King Theodore's Son' (sitter Prince Alámayou of Abyssinia), albumen print, 1868
Bibliographic References (Citation, Note/Abstract, NAL no)
Photograph featured in V&A web theme 'Treasures from Ethiopia' [http://www.vam.ac.uk/collections/periods_styles/hiddenhistories/ethiopia_treasures/index.html]
Cat. no. 1123, p. 456.
Cox, Julian and Colin Ford, with contributions by Joanne Lukitsh and Philippa Wright. Julia Margaret Cameron: The Complete Photographs. London: Thames & Hudson, in association with The J. Paul Getty Museum, Los Angeles and The National Museum of Photography, Film & Television, Bradford, 2003. ISBN: 0-500-54265-1
Weiss, Marta. Julia Margaret Cameron: Photographs to electrify you with delight and startle the world. London: MACK, 2015, p. 112.
Labels and date
Julia Margaret Cameron
Victoria and Albert Museum
Dèjatch Alámayou
Prince Dèjatch Alámayou was the son of an Ethiopian emperor who committed suicide rather than surrender to the British in 1868. The orphan prince came to live on the Isle of Wight, where Queen Victoria took a particular interest in him. Photographs of the prince were widely circulated and he was perceived as an exotic and romantic figure.
Given by Mrs Ida S. Perrin, 1939
V&A: 24-1939 [28 November 2015 - 21 February 2016]
Dèjatch Alamayou / King Theodore's son
Prince Dèjatch Alamayou was the son of Emperor Tewodros II, who committed suicide rather than surrender to the British during the Abyssinian Expedition of 1868. The orphan prince came to live on the Isle of Wight. Queen Victoria took particular interest in him, paying for his education and allowing him to be buried at Windsor Castle when he died of pleurisy aged eighteen.
Given by Mrs Ida S. Perrin, 1939
Museum no. 24-1939
[18 November 2014 – 25 September 2016]
Photographic paper
Albumen process
Subjects depicted
Portraits; Princes; Shields
African Diaspora; Black History; Portraits; Photographs
Prints, Drawings & Paintings Collection


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Banbury Football Festival
Two teams from Years 3 and 4 represented Queensway at the Banbury Football Festival on Thursday 16th October. Both teams did very well with Team B finishing 4th and Team A finishing 1st! Team A will now go on to represent Queensway in the finals which will take place in November. Well done to all the children who took part, and who represented the school brilliantly.


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Are you stuck with strands of old Christmas lights that don't work anymore? Or maybe you've heard about energy-saving LED lights and want to toss out your old traditional lights in favor of new ones? Perhaps you just don't decorate as much as you used to, and want to clear out some clutter.
No matter what the reason, resist the urge to throw old holiday lights in the trash. Christmas lights are made from copper, glass, and plastic—valuable materials that can actually be recycled and reclaimed. Recycling companies collect millions of pounds of old lights every year and ship them to reclamation facilities. The facilities will crush the lights, separate the individual materials, and then bundles those off for reuse. It's a way to "mine" existing products to reduce the need to go after virgin resources—which is why it makes environmental sense.
In addition to reducing waste, recycling your old lights gives you the chance to purchase new lights that are LEDs. ENERGY STAR certified LED holiday lights are 75 percent more energy efficient than incandescents. They last longer, too, because LED bulbs are not nearly as fragile as the thin glass bulbs on the old lights. Though the white LEDs take a little getting used to, the colored lights are just as delightful as the old mini lights you're recycling.
Plus, some organizations collect old lights, then make a small fee when they sell them to recyclers. They use the fees to help support their holiday charitable giving programs.
Ways to recycle old Christmas lights
Contact your city's municipal solid waste office. Many will recycle the lights if you drop them off, so give a call and ask. They may have even set up a collection day for old lights or be able to direct you to a local drop-off spot.
Ship your old lights to Holiday LEDS Recycling. Pack up your lights in a box, cushioned by some wadded-up newspaper, and ship them regular mail. Ask your neighbors if they'd like to ship their old lights, as well. The company says there's no reason to send the lights express. Once the company confirms shipment, they'll send you a coupon good for 15% off the purchase of new lights from HolidayLEDs.com. Please note that this program is only active until the end of January. The shipping address is: Holiday LEDS Recycling, 13400 Watertown Plank Rd., Suite 34, Elm Grove, WI 53122.
Drop off your lights at MOM's Organic Markets. If you live in Maryland, Pennsylvania, Virginia, or Washington, D.C., you can drop off your old lights at any MOM's Organic Market. MOM's will then pack up all the lights they receive and ship them to HolidayLEDs.com. Here's a full list of hours and MOM's locations.
Mail your lights to Christmas Light Source. This program is active all year long. Based in Dallas, TX, they work with a local recycling company that recycles the copper, glass, and plastic. The recyclers pay Christmas Light Source a small amount per pound of lights, similar to the way you get money in exchange for recycling aluminum cans. All proceeds from the Christmas lights recycling program are used to purchase educational books and toys donated to Toys for Tots in early December. Like the HolidayLEDs Recycling program, Christmas Light Source recommends you send them the lights the cheapest and slowest way. Their mailing address is: Christmas Light Source Recycling Program, 4313 Elmwood Drive, Benbrook, TX 76116.
At the end of the holiday season, get in the habit of shipping broken light strands to Christmas Light Source or Holiday LEDS rather than tossing them out or packing them up with the rest of your holiday decorations. Keep a box handy to pack the lights in as you take them off your tree or down from around the house. Then you can easily mail them off without much hassle. Perhaps each year, one person in your neighborhood can collect all the broken lights from nearby houses and get the lights shipped off for everyone.
Over time, as you replace your old lights with more durable LEDs, you'll end up saving time and money because you'll spend less shopping for and replacing old lights. Now that's something to feel festive about!
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Astronomy Archive
Village Sky At Night with Brian Watkiss
Sky of superlatives
Posted on January 20 2013 at 1:49:38 0 comments
Amateur astronomer Brian Watkiss peers into the Village night sky.
I know it's nearly two months ago, but I feel I must start these notes with a farewell to Sir Patrick Moore.
His love of astronomy was matched only by his love of a good party. The Universe is an emptier place and The Sky At Night will never be the same.
He did like to encourage people to go out and look at the night sky, though, and this month is the best time of year for doing just that, as it is full of superlatives.
The first thing that leaps out of the sky, directly south from about 7pm, is just about the largest constellation that is well-defined: Orion.
Looking much larger than it would appear from the accompanying chart, the three stars of the belt are immediately obvious, as are the stars of the sword hanging down from them. The middle star of these is actually a tight cluster of stars, one of which is the hottest star in the sky.
They all sit in front of, and illuminate, a cloud of gas, forming the Orion Nebula – visible as a sort of "fuzziness" around them. The top left hand star of Orion is the biggest star you will ever see: Betelgeuse.
This is the granddaddy of red giants and varies in a rather unpredictable way, as if it is getting ready to explode in a supernova.
Just below and to the left of Orion is the brightest star in the sky (unless Betelgeuse does erupt). This is Sirius in the constellation of Canis Major, one of Orion's hunting Dogs. This too, is a very hot star, giving it a distinctive blue colour.
Above and to the left of Orion, beyond Betelgeuse, is a pair of stars at the head of the zodiacal constellation Gemini. These two seem to generate the most amusement of all the stars but I have never understood why.
They are Castor and Pollux and the lower of the two, Pollux, is the smallest and nearest red giant in this part of the sky. It is known to have a planet the size of Jupiter orbiting it.
To the right, at a similar height above Orion, is the eye of Taurus the Bull. This is Aldebaran, the colour of which gives it away as another red giant. Higher and further right is the largest planet you will probably ever see.
This is Jupiter, certainly the brightest object in the sky and well worth viewing through binoculars to spot its four moons changing position on a daily basis.
Moving up and right a similar distance again, we come to what is, for me, the most beautiful sight in the sky. This is the Pleiades or Seven Sisters. Through binoculars, this little gathering of obviously blue stars can be seen to be sitting in the cloud of gas from which they were formed.
They are thought to be so young that the early dinosaurs would not have seen them (well, binoculars hadn't been invented then!).
Finally, from about the 6th, we get our best chance of spotting the smallest planet in our Solar System: Mercury. Start looking into the sunset from about 5.30pm but make sure the Sun has definitely set. Mercury will be very low and getting lower, disappearing by 6.30pm.
A very thin crescent Moon will be alongside on the 11th, which will help locate the planet – and by the 20th, it will, again, be too close to the Sun.
So get out and get to know our night sky. It's what Patrick would have wanted!
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For most of 2012 I did a printing course at the lovely Double Elephant in Exeter one day a week and have been experimenting with screen printing, drypoint, lino and collograph . It is very liberating and I am continuing with it into 2013 with a short break at the moment to catch up with myself in regards to content and graphics to be printed.
Here are a couple of the prints drypoint, lino and screen print.
I am excited about exploring and getting obsessive about detail in the drypoints and bold colour and graphics of the lino and screenprinting.
My practise is changing at the moment as I am opening my gilding workshop again to take in studio based work from antique dealers and designers in order to balance the books. It may well lead to some interesting combinations as I have always loved paintings on gilded panels i hope to try printing on them now. A new 21st century take on the icon tradition.
I bought a new domain name www.janefox.co.uk and have updated the site and added a new gilding page also.


text: I'm looking for advice on how to move from academia to IP or patent law. I've heard from multiple people (who all heard from a friend, who heard from a friend) that some law firms will hire Ph.D. scientists and help pay for law school, but I've never met anyone who has actually done this. I don't think I can quit my job to go to law school without some kind of guarantee that there's a job for me on the other side.


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Xiao Liuqiu【小琉球】or Little Liuchiu a small island located on the south-west of Taiwan, makes your summer vacation more lively. Enjoy!
If you are searching a place for a short trip and don't want to fly faraway to other tropical countries to enjoy sunshine and beaches, come to Xioa Liuchiou for a 2-3 day-trip.
We had limited time and tight budget for our vacation this time, but want to chillax at the beach in an island with easy access by public transportation. Xiao Liuchiu became our first choice.
Wanna know more full article now on Full Article – travelleben.com! WE NEED YOUR SUPPORT Your comment is very valuable to us. If you find this article useful or have any opinion, please share, subscribe, click "LIKE" or kindly let us know through ° Follow Travelleben https://www.facebook.com/travelleben https://www.instagram.com/travelleben https://www.travelleben.com so we could provide a better platform with complete information for you.
Music credits and all rights by Silence: • Follow Silience Facebook – http://facebook.com/momentofsilience Soundcloud – http://soundcloud.com/momentofsilience


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Aug 23, 2018
On today's episode of Just the Tips, Dean and I chat about the importance of taking breaks, going on vacation, leaving the grind behind and resetting. This is a really important episode for anyone who is feeling the burnout, or who is eyeing an upcoming vacation with anxiety because they feel like they just can't step away. Dean talks about how he sometimes realizes he's a bottleneck in his business that's unplugged once he's unplugged, and I tell a story about an epic vacation decision that demonstrates how important it is to surround yourself with the best people.
When we recorded this episode, I was just back from a vacation, and as I told Dean, even though I know it's true, I'm always surprised by how good vacations are for me. It can take a few days to reset, but eventually I get there. And Dean agreed, saying that for a time, he wouldn't allow himself to take vacations because he wasn't happy with where his business was at the time. So he'd just plow through, not recognizing a break would do him good. This is all part of something we've been talking about: When you own a business, the goalposts are always moving, so there's a good chance you're always going to feel there's "more work to be done." If this sounds like you, you need to tune into this week's episode of Just the Tips.
As you know, we're all about systems here. But Dean brought up a good point. When you have everything running on systems, theoretically it should be easier to step away. But you also become systematized, and it can be difficult to unplug yourself from your work. For me, it's less about feeling like I have to stick with the system, and more about the guilt I feel for not working. When you've been pursuing some sort of work for most of your life, the guilt is real when you take time away. And as Dean says, sometimes when he steps away, things progress rapidly and he realizes he was the one holding things back. That's more a Dean thing than mine thing, but still, it's a salient point. It's a less-talked-about aspect of owning a business, and an important discussion on this week's Just the Tips.
Dean and I chatted about how you empower people on your team both in your absence and in their day-to-day jobs. I told him this story of an executive coach I had early in my career who had a philosophy of a "six-inch putt." And what he meant was that it was his job to make the final decision that something was ready. So in golf terms, it was the team's job to drive the ball, chip it up onto the green, get it within six inches of the cup, and then when it's a sure thing, he can tap it in. But if it's not at that point, then it's not his job, and it is someone else's. So that's how you empower people, you get them to see their role as important to getting to that six-inch putt.
I told Dean this story about my vacation, where I was hanging out with a great group of friends who are all doing amazing things and really pushing boundaries. On the last night of the trip, we were in Albuquerque, and it was the birthday of one of the guys on the trip. So we were trying to figure out what to do for it, and I suggested something kind of crazy (Sorry, you have to listen to find out). Everyone quickly got on board, and we made an amazing few days happen. As Jim Rohn says, you will become the average of the five people you hang around with the most. This is a really great episode and fun conversation with a guy I try to limit my time with, Dean Holland. Check out this week's episode of Just the Tips.
Music for "Just The Tips" is titled, "Happy Happy Game Show" by Kevin MacLeod (http://incompetech.com) Licensed under Creative Commons: By Attribution 3.0 License
James P. Friel:
Dean Holland: