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High Throughput Screening
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White Papers
White Paper
Parallel Fed-batch CHO Culture on SciVario® twin, the Flexible Controller for All Your Bioprocess Needs.
Eppendorf performed fed-batch cultures using BioBLU® 1c and 3c Single-Use Vessels controlled in parallel by the new SciVario® twin bioreactor control system. This method highlights the SciVario twin's capabilities to run complex processes simultaneously using various sized vessels.
INTEGRA Bioscience
Automated RT-PCR set-up for COVID-19 testing
The emergence and outbreak of the novel coronavirus SARS-CoV-2 (COVID-19) has placed unprecedented demands on laboratories testing for COVID-19, leaving scientific staff to contend with a spiraling influx of patient samples and a rapid, continuous growth in workload.
Practical Applications of Data Integrity and Audit Trail Review
For many years there was little guidance on the expectations from auditors on how to ensure that these systems are in compliance and remain that way.
A rapid method to quantitatively screen bispecific antibody using Protein A and His1K biosensors
Bispecific antibodies (bsAbs) were first reported by Nisonoff and Rivers in 19611, however the engineering challenges associated with bsAbs have limited their development.
Thermo Scientific
Cryo-electron microscopy applications
Cryo-electron microscopy (Cryo-EM) allows the structural analysis of protein complexes flash-frozen in their nearnative states. You can now directly visualize not just large macromolecules but also smaller proteins complexes, including membrane proteins.
Reaction Biology
The Hollow Fiber Model: In Vivo Screening Assay to Speed Up Your Drug Discovery Process
Efficacy testing with confidence
Use the Hollow Fiber Model to select the best drug candidates and tumor models for subsequent xenograft tumor testing with higher predictability than conventional assays.
Discover how SMC™ technology can support all phases of immunogenicity testing
using digital counting on the SMCxPRO™ high-sensitivity instrument for low-level protein detection.
Charles River
In Search of a Disease-Modifying Agent for Parkinson's Disease
In this white paper, we review a successful small molecule drug discovery project conducted by a team of scientists from Genentech and Charles River that spanned hit identification and lead optimization of a highly potent LRRK2 inhibitor.
LC-MS/MS Makes Comprehensive Cell Culture Media Analysis Fast, Easy and Effective
Ensuring that the cell culture media is comprised of the optimal formulation for growth and is free of impurities is vital to the success of biopharmaceutical development. This e-book presents one solution for measuring all key media components.
Accelerating Biotherapeutic Discovery
By reading this white paper you will learn about the challenges associated with the discovery and development of therapeutic antibodies – an especially promising and lucrative field for the biopharmaceutical industry. The white paper discusses the benefits of using large antibody panels for screening and interrogating the epitope diversity of the whole panel in early-stage using Array SPR (Surface Plasmon Resonance) to accelerate discovery. Researchers can now realize the full kinetic and epitope potential of their antibody libraries.
Download this white paper to learn how Array SPR kinetics and epitope binning assays will improve your drug discovery efforts.
A study of Interleukin-2:. stability and behaviours in solution
Here we conduct a study into the behavior and stability of IL-2 using microfluidic diffusional sizing (MDS) in order to observe native state, in-solution responses. The tendency to oligomerize at varying concentrations, and the stability (particularly propensity to aggregate) under changing buffer conditions are investigated.
Stunner - The one and only biologics quantification and quality combo.
Stunner pulls double-duty so you don't have to. It's the only system out there that takes UV/Vis concentration and Dynamic Light Scattering (DLS) size measurements from the same sample at the same time. Nail down your protein quality by knocking concentration, hydrodynamic size, polydispersity, and detection of aggregates off your list in one shot. Without skipping a beat, you can also measure the colloidal stability of your samples or design your own custom apps.
Labguru helps ADC Therapeutics increase productivity
Labguru is a secure, compliant, web and cloud-based, Electronic Lab Notebook (ELN) that enables biotech, life sciences, and pharma companies, as well as academic institutions, to run their labs more efficiently and maximize their research output. It's trusted by over 30,000 users worldwide who rely on it to plan and document experiments, track progress, streamline lab logistics and share results.
Ultra-Low Temperature Freezers
Independent test results confirm the new VIP ECO® upright ultra-low temperature freezer has the lowest total daily energy usage in its class in the industry. Download the white paper to get more details of key performance metrics from independent ENERGY STAR® testing.
Download the White Paper
Is Your DART Study Set Up to Fail? Download whitepaper.
Taking place at a critical juncture in drug and vaccine development, Developmental and Reproductive Toxicology (DART) is a highly specialized science that companies simply cannot afford to get wrong. Getting the science right is extremely critical. Is your DART program subject to critical missteps that will place you at risk of program failure? Here's how to tell.
Efficient CRISPR/Cas9 Delivery Using Nucleofector™ Technology: Comparison of Plasmid- and RNP-based Editing
How does the delivery of CRISPR/Cas9 plasmids compare to ribonucleoproteins (RNPs)? A Lonza customer shares a protocol using the non-viral Nucleofector™ Technology for comparing NHEJ and HDR frequencies resulting from CRISPR/Cas9 delivered as plasmids or RNPs. Download the white paper:
Antibiotic resistance is a serious, globally extensive problem that significantly affects patient morbidity and mortality. Here, we discuss the emerging threat of antibiotic resistance and the importance of clinically relevant reference materials for use in assay development and drug discovery.
Read more>>
Recombinant eSpCas9 Protein - For RNP-Based Genome Editing
The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)
system was discovered in bacteria, where it functions as an adaptive
immune system against invading viral and plasmid DNA. In this system,
short DNA sequences (spacers) from invading viruses are incorporated at
CRISPR loci within the bacterial genome and serve as memory of previous
Detecting Neurobehavioral Effects
Neurobehavioral testing provides critical insights into how drugs and chemicals affect the nervous system. This Whitepaper covers the considerations in the Design and Execution of Neurobehavioral Studies.
Comparison of common fluorescent labels for liquid chromatography analysis of released N-linked Glycans
Glycosylation of biotherapeutics is a frequently monitored critical quality attribute. N-Glycan analysis typically involves release and derivatization to a fluorescent label. Workflow and analysis are impacted by label choice. Click for more
Pipette Routine Check – Best Practices
Pipettes should be calibrated annually to maintain their perfomance. In the interim, it is recommended to perform a routine check to test the functionality of your pipettes in order to be confident with your pipetting results. Follow the guidelines below to properly perform such a routine check.
The Tip of the Iceberg: How Pipette Tips Influence Results
This study including tips from 15 different manufacturers proves that a pipetting system working perfectly with a certain tip exceeds the permissible error tolerances when a tip from a different manufacturer is used.
R&D Systems
A Novel Method for Detecting Terminal Glycans Using SDS-PAGE
We have developed a novel technique capable of probing terminal monosaccharides on proteins using SDS-PAGE. This technique does not require expensive equipment and is more specific than comparable methods. Additionally, it offers any laboratory the ability to gain a greater understanding of the glycan profiles of their protein of interest without significant investments.
Monitoring Viral Infection of Mammalian Cells using Digital Fluorescence Microscopy
Here we demonstrate the ability of the Cytation™ 5 Cell Imaging Multi-Mode Reader and Gen5™ Microplate Reader and Imager Software to determine infection levels of HIV with HeLa cells in culture.
Hera BioLabs
Novel gene edited rat models exhibit enhanced immunodeficiency and engraftment of human tumors. Rats can produce larger samples for analysis and in many ways are more physiologically similar to humans than mice. Applications discussed include: efficacy and toxicity in a single animal, more precise surgical manipulations and better in-vivo imaging
The imaging and analysis of fluorescently stained cells has traditionally been accomplished using manual microscopic methods with low numbers of samples. Here we describe the use of the Lionheart™ FX Automated Live Cell Imager and the CellASIC ONIX2 perfusion micro-incubator to rapidly image and analyze perfused tissue culture cells in multiple fluorescent colors and brightfield.
The Jackson Labs
NSG™* and NSG™-SGM3* mice are a proven host for engraftment of human tumors or establishment of human immunity following hematopoietic stem cell transplantation. Here, we provide evidence that NSG and NSG-SGM3 mice...(click for more)
BioStatus Limited
Real-Time Cell Health Monitoring, Cell-by-Cell, in 2D and 3D in the Far-Red.
In flow cytometry and fluorescence microscopy (including high content screening), a reliable estimation of cell viability is important because it is central to assays for apoptosis and in vitro toxicology. Likewise, it is a measure ... (click for more)
Swift Biosciences
Next-generation sequencing (NGS) of bisulfite-converted DNA to detect methylation status with per-base resolution is currently restricted by input requirements, requiring at least 50 ng of DNA. This Application Note shows how the unique chemistry of the Accel-NGS® Methyl-Seq DNA Library Kit enables the construction of high complexity libraries for ....(click for more)
TTP Labtech
Multiplexed fluorescence ELISA assays on mirrorball®
Enzyme-linked immunosorbant assays (ELISA) can be used in multiple stages of the drug discovery process to help identify and measure the cellular responses to therapeutic or toxic molecules.....(click for more)
Validation of High-Throughput Wound Healing Assay using 3D Cell Patterning and Automated, Kinetic Imaging
Here, we demonstrate a novel 3D wound healing assay model that can overcome 2D assay limitations. The method incorporates magnetic levitation (click for more)
Agilent Technologies
White Paper: Why Reversed-Phased?
Protein biopharmaceuticals are very heterogeneous, so a number of chromatographic techniques may be required to fully characterize and a ctive pharmaceutical ingredient (API). Methods include size exclusion chromatography for the quantification of dimers and aggregates, and ion-exchange for the indentification of charge variants. Click for more…
Agilent Technologies
Application Note: Biologics and Biosimilars
Size exclusion chromatography (SEC) is an important tool for monitoring monomers,
dimers, aggregates, and potential degradants in biotherapeutic protein samples,
including monoclonal antibodies and their derivatives. As aggregation is considered
a critical quality attribute, quantitation is required. Click for more...
Agilent Technologies
Application Note: Biologics and Biosimilars
Monoclonal antibody aggregation can arise from multiple mechanisms, including
product and environmental factors. Size exclusion chromatography (SEC) is a
standard method to determine and quantify aggregation and fragment levels of
monoclonal based therapeutics. Click for more...
Agilent Technologies
Application Note: Biotherapeutics and Biosimilars
N-linked glycosylation is a critically important and very complex post-translational
modification. It therefore needs to be controlled and monitored throughout
development, processing, and manufacture of drug glycoproteins. Click for more.
Simultaneous 96-well Extraction of Urine Samples for Drugs of Abuse Analysis
INTEGRA, in conjunction with DPX Labs LLC, has produced an application note that describes a novel high throughput semi-automated sample method for processing urine samples ready for drugs of abuse analysis by LC-MS/MS. Using an INTEGRA VIAFLO 96 electronic pipette with DPX mixed mode tips, a single 96-well plate loaded with samples can be extracted and ready for LC-MS/MS analysis in less than ten minutes eliminating the need for a time-consuming evaporation step.
For a copy of this new application note please click here
Jackson Labs
JAX® offers humanized NSG™ and NSG™-based strains with additional manipulations
of the host (including the NSG™-SGM3 strain that expresses the human cytokinesIL-3, GM-CSF, and SCF) for an enhanced ability to recapitulate specific functions ofhuman disease in vivo.
Trevigen's Cultrex® Basement Membrane Extract (BME)for in vitro Organoid/Spheroid
There is a growing recognition of the role of the extracellular matrix (ECM). The ECM is the biologically active macromolecular component present in all tissues and organs. It not only provides structural support for tissues and organs but contains information that controls cellular programs, differentiation, and morphogenesis. It is not surprising that different tissues and organs contain different ECMs
EMD Millipore
Application Note:
High resolution imaging flow cytometry provides comprehensive analysis of live mixed algae cultures and assesses high value commodities in algal biomass.
Haley R. Pugsley and Benjamin E. Alderete
Jackson Labs
NSG™* mice are a proven host for engraftment of human tumors or establishment of human immunity following hematopoietic stem cell transplantation. Here, we provide evidence that humanized NSG mice can support the growth of allogeneic human tumors.
EMD Millipore
ImageStream® X Measurement of immune cell function using ImageStream® X Cytometry
Cells of the innate and adaptive immune system defend the host from pathogens while remaining tolerant to self antigens, and dysregulation of the immune system can result in autoimmunity or infection. Immune cells sense their environment by binding and processing soluble mediators of inflammation or through direct contact with other cells, resulting in signal transduction and activation or suppression of effect or function.
Thermo Fisher
Many biological science applications require an accurate, precise measurement of the number of cells and their viability prior to downstream analysis. This assessment
can be done manually with a traditional hemocyotmeter and microscope or with an automated cell counter, such as the Invitrogen™ Countess™ II FL.
Computer-aided Assay Development for Charge Heterogeneity Analysis by iCE
The goal of this note is to promote the successful application of DOE tools in the assay development process by offering a stepwise example. The road map contained in the following pages has purposely catured enough technical detail to provide a comprehensive reference guide for both the statistician and analytical biochemist. The subjects that
will be covered include initial factor screening, construction of a central composite DOE, response surface modeling, assay optimization, model validation and assay performance.
EMD Millipore
Application Note:
Measuring autophagic flux by assessing LC3, p62 and LAMP1 co-localization in Jurkat cells using imaging flow cytometry.
Haley R. Pugsley, Vidya Venkatachalam, Sherree L. Friend, Raymond Kong, Shobana Vaidyanathan, Phil Morrissey, EMD Millipore
Jackson Labs
Humanized NSGTM* mice are used by researchers and drug discovery scientists as powerful tools to study hematopoiesis, inflammatory disease, viral host-pathogen interactions, and are accelerating the development of novel therapies in oncology. How to select the appropriate model for your research are important considerations for conducting impactful research using humanized mice and are the focus of this paper.
Handheld Pipetting - Best Practices
For the vast number of scientists that undertake handheld pipetting protocols - INTEGRA has produced a highly informative illustrated 'Best Practices' guide. Drawing upon decades of pipetting experience - INTEGRA has compiled an unbiased series of hints and tips to help you improve your handheld pipetting. Expert advice is provided in areas including before you start, how to pipette and optimising pipetting performance. Each area of best pipetting practice discussed is backed up with an explanation of why this produces optimal results
Commonly used air displacement pipettes are highly accurate for most liquid dispensing applications. However, the viscosity, volatility, surface tension, and temperature of the solution can adversely affect the performance of these air displacement pipettes.
Click here to read more
Trevigen's Extracellular Matrix Proteins for Triculture of Breast Cancer Spheroids, Endothelial Tubules, and Human Mesenchymal Stem Cells
Here we describe the 3D triculture model which recapitulates the breast cancer tumor
microenvironment. Such a model will be useful for drug screening, basic research, and personalizedmedicine providing one of many needed tools to facilitate more physiologically relevant assessmentsof cancer cell behavior.
Register for the Illumina Genomics Symposium
Next-Generation Sequencing Applications for Neurological Disorders
Today, cell biologists are looking to new methods, like next-generation sequencing, to investigate the genetic underpinnings of disease biology and generate hypotheses for further studies that analyze gene expression and protein function.
New paper
Breaking the Bottleneck: Boosting Lab Performance with Automated Plate Seal Removal
Removing and/or replacing hundreds of plate seals at a time can represent a formidable and painstaking challenge. This paper reviews how high-throughput screening (HTS) and high-content screening (HCS) laboratories discovered hidden productivity improvements using new adhesive-based de-sealing technology.
Gilson, Inc.
Streamlined Purification of Assay-ready Oligonucleotides by Automated HPLC
High Purity Oligonucleotides are used for standard molecular biology techniques such as qPCR, Next Generation Sequencing, and microarray analysis. Full-length products need to be separated from truncated sequences, inhibiting salts, and base protecting groups. Gilson has developed a liquid handling platform configured with automated preparative and analytical HPLC capabilities to isolate, analyze, and desalt reagent-grade oligonucleotides in one continuous run.
Gene Synthesis Handbook: Applications and Technologies for DNA synthesis
36-page technical introduction to designing, constructing, and using synthetic genes. What is gene synthesis? How does it differ from traditional molecular cloning? Why is gene synthesis a revolutionary tool, and how is it performed? Includes: case studies from cancer, structural biology, vaccine development, neuroscience, plant biology, and synthetic biology; oligo design tools and assembly methods; and 54 peer-reviewed references.
Trevigen's γ-H2AX Pharmacodynamic Assay
Here we describe an ELISA based Pharmacodynamic Assay (PDA) to measure the formation of γ-H2AX. Such an assay will be useful for drug screening, basic research and upcoming clinical trials providing one of many needed tools to support hypothesis-driven drug design strategies.
The Effect of Cell Culture Method on Long-Term Primary Hepatocyte Cell Health
BioTek Instruments, Inc.
Features BioTek's MultiFlo™ FX Microplate Dispenser and BioTek's Cytation™ 3 Cell Imaging Multi-Mode Microplate Reader
Cytation™ 3 Cell Imaging Multi-Mode Microplate Reader
Cytation™ 3 is a cell imaging multi-mode microplate reader that combines automated digital microscopy and conventional microplate detection. This patent pending design provides phenotypic cellular information with well-based intensity data.
MultiFlo™ FX Microplate Dispenser
MultiFlo FX is a modular reagent dispenser for 6- to 1536-well plates, dispensing volumes as low as 500 nL. An available wash module adds multi-purpose functionality.
Quanterix Corporation
Scientific Principle of Simoa™ (Single Molecule Array)
Quanterix' ultra-sensitive diagnostic platform, the Simoa HD-1 Analyzer is capable of measuring individual proteins at concentrations 1000 times lower than the best immunoassays available today. The Single Molecule Array (Simoa™) technology at the heart of the platform enables the detection and quantification of biomarkers previously difficult or impossible to measure.
Bio-Rad Laboratories
The story of Leukemia research is being re-written—one droplet at a time. See how droplet digital PCR gives scientists the power to detect what was previously undetectable. Watch Video
Magnetic Bead Based Soil DNA Isolation using the epMotion® and ClearMag™ Technology
Many nucleic acid purification applications have been previously automated on the Eppendorf epMotion 5075. The exception is purification of DNA from difficult samples high in PCR inhibitors, including soil, turbid water, biofilms, and human gut samples. While highthroughput purification of DNA can be achieved using 96-well silica plates, this method is time-consuming and not hands-free. This Application Note describes the successful automation of DNA isolation from these sample types with magnetic bead-based technology from MO BIO Laboratories.
BioTek Instruments, Inc.
of HTRF® elF4E Kinase Assay Using a 3D Tumoroid-Based Cell Model
MultiFlo™ FX is a fully modular, automated reagent dispenser for 6- to 1536-well plates offering BioTek's unique Parallel Dispense™ technology - both peristaltic and syringe pump operation down to 500 nL. An available microplate wash module adds multi-purpose functionality. A fully configured MultiFlo FX replaces up to five liquid handlers.
Quanterix Corporation
Scientific Principle of Simoa™ (Single Molecule Array)
Quanterix' ultra-sensitive diagnostic platform, the Simoa HD-1 Analyzer is capable of measuring individual proteins at concentrations 1000 times lower than the best immunoassays available today. The Single Molecule Array (Simoa™) technology at the heart of the platform enables the detection and quantification of biomarkers previously difficult or impossible to measure.
Knowledge is Power: Why Using ATCC's Annotated, Authenticated Tumor Cell Lines for Cancer Research Matters.
The availability of sequencing data is making it possible to use cell lines to tie oncogenic events to their underlying genetic cause and to examine cancer from new perspectives. However, given the potential cost of using cross-contaminated materials it is important to start your experiments with authenticated tumor cell lines or panels, such as those available from ATCC.
More info at 703-365-2700 firstname.lastname@example.com
BioTek Instruments
Multiplexed Assay for IL-6 Secretion and Cell Viability Using an Epithelial Ovarian Cancer Cell Line
Using the Cytation™3 Cell Imaging Multi-Mode Reader to Monitor IL-6 Secretion Using HTRF Detection and Cell Viability Using Digital Widefield Fluorescence Microscopy
Eppendorf ThermoMixerTM C – Features, Options, Applications
Katrin Käppler-Hanno, Eppendorf AG, Hamburg, Germany
Executive Summary
Primarily used in the field of life sciences, the Eppendorf ThermoMixerTM C is a device for the temperature control and mixing of liquids or samples. The device offers a wide temperature control range (1 °C–100 °C), high flexibility with the use of diverse tube and plate formats and advanced mixing performance with the 2DMix-Control technology and a mixing speed of 3000 rpm. With the new technology
the Eppendorf ThermoTop® prevents condensation on tube lids or plate sealing foils.
mAb Discovery Engine
has developed an antibody engine (mAb discovery engine) for discovery of antibodies to transmembrane proteins like G-protein coupled receptors (GPCRs). The mAb discovery engine generates panels of diverse antibodies in terms of sequence, somatic hypermutation, epitopes, and performance. The mAb discovery engine is a platform that is capable of routinely generating antibody candidates that are diverse, functional and novel.
Mirus Bio, LLC
From Reprogramming to Differentiation – Transfection Applications for Stem Cell Research
Differentiation and reprogramming techniques are imperative in stem cell research and require virus-free applications for biotherapeutic and new drug development. Mirus Bio presents a suite of
IT® Transfection Reagents and an Ingenio® Electroporation Kit that are high efficiency, low toxicity DNA and RNA delivery solutions validated for a variety of stem cell applications.
Application Note
: Cardiotoxicity
This application note describes the utility of the Erenna Immunoassay System and the Erenna cTnI Assay towards early assessment of cardiotoxicity in rats. Due to its unique highdefinition technology, elevated levels of cTnI in serum can be detected earlier with the Erenna Immunoassay System
Hamilton Company
Rapid, High Throughput Extraction and Purification of Genomic DNA from Whole Blood Using the Akonni TruTip® Extraction System on the Hamilton Microlab® STAR Liquid Handling System
Akonni Biosystems has developed the simple and effective ultra-rapid TruTip® automated extraction method designed to isolate genomic DNA from low volumes of whole blood for downstream genetic testing including any type of PCR, sequencing and microarray detection. The tip-based extraction technology is paired with the advanced, proven and reliable Hamilton Microlab STAR liquid handling system for high throughput sample processing.
Applied Photophysics Ltd
Automated Circular Dichroism with Dynamic Multimode Spectroscopy: a powerful tool for establishing conformational stability of cloned proteinsn
Abstract The relative stabilities of the 9th-10th
type III domain pair of fibronectin (FIII9-10) and two
mutants thereof, all of which have immunoglobulin-like beta-sandwich domains, were investigated using dynamic multimode spectroscopy (DMS) with automated circular dichroism (ACD). It is shown that the mutants are significantly more stable than the wild-type, that the folded and unfolded protein conformations for all variants are similar, and that aggregation accompanies unfolding.
Guidelines for Routine Use and Maintenance of Ultra-Performance Size-Exclusion and Ion-Exchange Chromatography Systems
Paula Hong and Kenneth J. Fountain
To decrease the frequency of system repairs and contamination, a series of steps have been outlined for maintenance and care of a chromatographic system and columns used for the analysis of biomolecules.
Evolving Intelligence
By Wendy Quinn-Decatur,
M.S.I.S., Associate Librarian, AMRI
As Contract Research and Contract Manufacturing Organizations (CROs and CMOs) continue to evolve and provide increasingly important contributions to their customer's discovery and development priorities, implementing a customer-focused approach to corporate library services can turn a repository for dated literature into an invaluable information center for the library's constituents.
Waters Corp.
Application Note
Metabolite Identification Workflows: A Flexible Approach to Data Analysis and Reporting
Gilson, Inc
Analyzing catecholamines and their metabolites in patient urine samples is important for sympathoadrenal system tumor diagnosis. This application note discusses the automation of sample preparation and solid phase extraction (SPE) with the CIinRep Complete Kit (RECIPE Chemicals + Instruments GmbH) using the Gilson ASPECTMXL or GX-274 Liquid Handler. Following SPE, fractions were analyzed via HPLC for analytes VMA, HVA, and 5-HIAA using an electrochemical detection (ECD) method.
Applied Photophysics Ltd
Protein Stability by Chemical Denaturation
Abstract Automated Circular Dichroism (ACD) was used to determine the thermodynamic stability of a protein by chemical denaturation.
Incubation of samples ensures equilibrium is reached, eliminating systematic error
► Short path lengths extend the accessible wavelength range
► Automation significantly increases productivity and reduces human error
INDIGO Biosciences, Inc.
Retinoid Receptors: Overview and Tools for Researchers
Retinoids are a class of chemical compounds related to vitamin A. The two predominant and most studied retinoids are all-trans retinoic acid (ATRA) (the acid form of vitamin A also known as Tretinoin) and 9-cis-retinoic acid (9-cis RA or Alitretinoin). Retinoids have many important and diverse functions throughout the body, including roles....
Bio-Rad Laboratories
Effect of Conductivity on DBC on CEX Resin
In an effort to improve the antibody manufacturing platform, newer cation exchange resins were evaluated in this study. Dynamic binding studies were conducted using 2 different monoclonal antibodies at a stable pH and varying conductivities across nine resins to determine the differences in binding capacity
Shimadzu Scientific Instruments
The Perfinity Workstation: Automating Mass Spec Sample Preparation
This paper presents the Perfinity Workstation, a fully-integrated online protein sample preparation workstation, combining the selectivity of immunoassay approaches with the resolution of HPLC. It utilizes multiple proteomic approaches to rapidly and reproducibly perform affinity selection, buffer exchange, trypsin digestion, desalting of the sample and reverse phase LC analysis. All of these processes enable the generation of purified peptides ready for LC-MS analysis in about 10 minutes, and result in peptide maps of exceptional reproducibility (<10% CVs).
Gilson, Inc
This application note provides a quick comparison of three pharmaceutical compounds using high pressure normal phase and low pressure flash chromatography on the Gilson PLC 2020 Personal Purification System. Comparison of peak shape, peak retention time, chromatography run time, fraction volume, and solvent consumption were all studied to determine the most efficient and optimal chromatography method.
Bio-Rad Laboratories
Composite Profiling of Angiogenic Factors Using Bio-Plex Pro Human Cancer Biomarker Panel 1
The Bio-Plex Pro human cancer biomarker panel 1 is a unique blend of magnetic bead-based multiplex assays designed to meet the needs of the most discerning preclinical and clinical researchers. The multiplex format enables robust and reproducible measurement of 16 soluble proteins involved in disease processes such as: Angiogenesis, Metastasis, Cell proliferation, Cell adhesion/migration, Apoptosis and Inflammation
Applied Photophysics Ltd
Strategies to compare far-UV Circular Dichroism spectra of similar proteins using Chirascan™-plus ACD
Circular Dichroism (CD) spectroscopy is an extremely sensitive technique to analyse the conformation of proteins in solution and can be used as a fingerprint of a particular protein structure [Greenfield, 2006]. Due to the very low sample volumes required and the simplicity of the measurement, it is a powerful comparative tool to detect differences in structure between proteins.
This paper discusses how Chirascan™-plus ACD presents easy-to-obtain, highly reproducible results while achieving high throughput, previously not available for Circular Dichroism. Using advanced robotics combined with the 96 or 384-well plates, Chirascan™-plus ACD can rapidly prepare many replicates and perform multiple measurements for clear determination of standard deviations.
Stellaris RNA FISH: Detection, Localization, and Quantification of mRNA at the Cellular Level
Presented here is a new method for in situ RNA detection, the Stellaris™ FISH technology, which enables simultaneous detection, localization, and quantification of individual RNA molecules at the cellular level. This technique provides a high degree of specificity and sensitivity, is ideal for applications that analyze transcription site activity, protein- RNA interactions and mRNA translocation events, and holds promise for future diagnostic applications.
Large Custom Databases
Unlocking the Potential of your Chemistry Investment
Too often, virtual screening has failed to deliver useful results. The data from your past projects is high quality, but may be focused on the wrong area and lack diversity.
This new white paper from InhibOx describes how companies can build and rapidly search very large, high quality virtual libraries, starting from their own reagent collections and chemistries, providing a platform for successful virtual screening.
JMP Clinical
A Primer on JMP® Clinical Incidence Screens
JMP Clinical has a number of analytical processes (APs) that calculate and compare the incidence of events between two or more treatment groups in a clinical trial. Interventions Incidence Screen enables you to compare the incidence of concomitant medications or substances. AE Incidence Screen enables you to compare the incidence of adverse events. DS/MH Incidence Screen enables you to compare disposition events (e.g., randomization or completion status) or medical history terms (which, technically, is a comparison of prevalence). Finally, AE Resolution Screen enables you to examine the resolution of adverse events. Most of these APs operate in a similar manner. In this white paper, we use AE Incidence Screen with sample data to illustrate the common functionality of these APs. We also point out important differences among the other APs at the end of the example
Lucigen Corporation
Cloning and Protein Expression Systems
enable you to effortlessly clone your gene and express protein the next day. PCR amplicons are directly mixed with cloning-ready
vectors and competent cells to yield >90% recombinants, with no ligation step. Expression is tightly controlled. The optional SUMO fusion tag increases solubility.
EMD Millipore
The Importance of Being Profiled: Improving Drug Candidate Safety and Efficacy Using Cross-target Profiling
Profiling of putative lead compounds against a representative panel of important enzymes, receptors, ion channels and transporters is an excellent way to establish a preliminary view of potential issues that might later hamper development. An early idea of which off-target activities must be minimized can save valuable time and money during the preclinical lead optimization phase if pivotal questions are asked beyond the usual profiling at hERG. The best data for critical evaluation of activity at GPCRs and ion channels is obtained using functional assays, since binding assays cannot detect all interactions and do not provide information on whether the interaction is that of an agonist, antagonist, or allosteric modulator. This overview discusses the benefits of in vitro assays, specific decision points where profiling can be of immediate benefit, and highlights relevant profiling services offered by EMD Millipore.
INDIGO Biosciences
Live Cell Multiplex Assay
For use in combination with INDIGO's
3x32-, 2x48-, or 1x96-well format
Nuclear Receptor Reporter Assay Systems
SABiosciences, a QIAGEN company
RT2 Profiler™ PCR Arrays
Pathway-Focused Gene Expression Profiling with qRT-PCR
This paper evaluates the performance of the newest technique for monitoring the expression of a panel of pathway- or disease-specific genes: the RT2 Profiler PCR Array System from SABiosciences. The PCR Array System combines the quantitative performance of SYBRR Green-based real-time PCR with the multiple gene profiling capabilities of a microarray. The PCR Array is a 96- or 384-well plate containing RT2 qPCR Primer Assays for a set of 84 related genes,
plus five housekeeping genes, and three controls.
Trevigen, Inc
DNA Repair Deficient Cell Lines- Tools to Study Genomic Instability- White Paper
DNA repair proteins maintain the stability of the genome. When repair protein function is impaired through mutations, the genome may become unstable which is a hall mark of solid tumors. Trevigen, Inc. is pleased to announce the availability of nineteen DNA repair gene knockdown cell lines, encompassing the Base Excision Repair pathway. The availability of this panel of target specific LN428 glioblastoma shRNA lentivirus transduced cell lines will permit scientists to study the molecular etiology of tumor genomic instability and to exploit it in oncology research.
Propelling GPCR drug Discovery through innovative products
Cutting edge portfolio and how it has led to new learnings
Caliper Life Sciences
Fragment-Based Screening using the LabChip EZ Reader - White Paper
Fragment-based lead discovery is establishing itself as valuable approach for the discovery of novel small molecule leads. It relies on establishing a better understanding of the binding mechanism of a ligand for its binding partner by probing for "hot spots "on the protein target with small molecule fragments (typically <300 Daltons). Its simplicity is in the principle of building a pharmacophore from the ground up. Successful selection requires data on binding affinity as well as extensive SAR information provided by a variety of techniques . . .
Caliper Discovery Alliances & Services
CDAS has created GPCRFunctional™ Label Free service programs to offer more relevant assay development, screening, drug activity and receptor characterization studies in a more physiological cellular context.
Ariadne Genomics
Mechanistic Disease Modeling
Developing mechanistic models that can help drive hypotheses and explain disease and its progression is a challenge for drug discovery and development companies. This white paper discusses how an in silico approach to defining punitive disease models can assist researchers in their efforts to find novel drug targets, elucidate drug toxicities and find new indications for existing therapies.
Gilson, Inc
Pipetting Hot and Cold
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MD Biosciences, Inc
Progressing pipelines withPre-clinical CRO Partnerships.
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Invitrogen by Life Technologies
Measure inhibitor binding to non-activated kinases
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Invitrogen by Life Technologies
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Generation PARP in vivo Pharmacodynamic Assay:
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Adeptia Inc.
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The Netflix hit Orange Is the New Black has won over critics and viewers alike this summer. The original series follows a diverse cast of characters in a women's prison in upstate New York. One of the breakout stars is Laverne Cox. Her character, Sophia, is a transgender woman who stands up for herself among prison officials and other inmates.
Cox spoke with Tell Me More host Michel Martin about the show and her experience as a transgender woman and actress.
Interview Highlights
On Cox's gender transformation
Cox brings insight from her own experience to the role. "My third-grade teacher, Ms. Ridgeway, called my mom and said, 'Your son will end up in New Orleans wearing a dress if we don't get him into therapy right away.' " Cox said she only met other transgender people after she moved to New York for college.
And although her transition was not an easy process, she says she comes more into her own every day. "Through all the things — the difficulties of what it means to be who I am, I love myself. And I am so grateful that I get to live authentically now."
On the role of Sophia
"When it came along, I thought, what a wonderful opportunity to talk about and highlight issues of trans women in prison. Certainly, Sophia has been one of the most complicated characters I've gotten to play as an actress, and I'm really grateful she's come into my life."
On acting while black and transgender
"It's hard. The issue of not just being trans, but also being a woman, and it's being black. And the industry historically doesn't think that we are marketable, or they want to cast us in very limited ways. But I think that the wonderful lessons that Orange Is the New Black is teaching us is that it shows our industry — the entertainment industry — that you can cast women of different races, you can cast different ages and body types, and folks will tune in and be interested. And the public is craving that."
On prostitute roles
"When folks want to write a trans character, the first thing that they think of is sex work. And part of the reason is that the most visibility, really, that trans folk get is through sex work. And then there is also crazy unemployment rates among transgender people; it's like twice the national average. If you're a trans person of color, it's four times the national average. So, so often the only job opportunities presented for trans folks are in street economies, including sex work. Obviously there are lots of trans women who don't do sex work, who have all kinds of professions."
On transforming the image of transgender people
"A beautiful blog [post] was written by this woman who said that she didn't really approve of the transgender thing and had all these misconceptions about trans people, and Sophia's storyline changed that for her."
Every new TV show has somebody somewhere willing to tout it as the next big thing. But every now and again a new show comes along that grabs critics and the public alike and they can't stop talking about it. This year, that show is called "Orange Is the New Black." It's a Netflix original series that premiered last month. It follows a diverse cast of characters at a women's prison in upstate New York. And one of the breakout stars is Laverne Cox. She plays Sophia, a transgender inmate, in this scene, she's complaining to a prison official about losing an important prescription.
MICHAEL HARNEY: (As Sam Healy) The bottom line is that the prison can no longer provide high-end hormones.
LAVERNE COX: (As Sophia Burset) If I don't get my medication, I'm going through withdrawal, hot flashes, night sweats. My face will sag, my body hair will start to go back.
HARNEY: (As Sam Healy) OK. We don't need to get into that.
COX: (As Sophia Burset) Let me explain this for you.
MARTIN: And Laverne Cox is with us now to talk about this groundbreaking role and her other work, we hope, as a transgender actress and activist. Welcome. Congratulations.
COX: Thank you so much, Michel. It's a pleasure to be here.
MARTIN: I understand that you were actually researching the experience of trans women in prisons when your agent told you about this role. Do I have that right?
COX: That is correct, yes. There is a woman named CeCe McDonald who's in prison. She's serving a 41 month sentence in Minnesota for defending herself against a racist and transphobic attack.
MARTIN: And why were you doing that research?
COX: For a show called "In the Life," which it's a PBS show called - that existed for about 25 years, and last year, they lost their funding. So I was producing a segment where I was planning to go to Minnesota to interview CeCe McDonald. So I was doing a lot of research about her case and specifically about trans women in prison and the issues that affect us.
MARTIN: Yeah, so how interesting that then this role comes along.
COX: I really don't believe there are any accidents. When it came along, I thought, what a wonderful opportunity to talk about and highlight issues of trans women in prison. Certainly, Sophia has been one of the most complicated characters I've gotten to play as an actress and I'm really grateful she's come into my life.
MARTIN: It's only fair to mention that everybody might not know what it means to be transgender. So would you please explain for people who don't know what we're talking about.
COX: Someone who's transgender is someone whose gender that they're assigned at birth does not match their gender identity, how they see themselves. Just because someone will determine that you're a female at birth based on, you know, your genitalia, but you might not necessarily identify as female and so that just means that you're transgender.
MARTIN: And so you were born a biological male. At what point in your life did you embrace a feminine identity and to begin to live as a woman? And were you already in your profession at that point?
COX: When I was in third grade, my third grade teacher Mrs. Ridgway (ph) called my mom and said that your son will end up in New Orleans wearing a dress if we don't get him into therapy right away. So I was always very feminine. And so it's something that, after those therapy sessions and throughout my adolescence, I tried to suppress. When I finally moved to New York - I grew up in Mobile, Alabama - but when I finally moved to New York and I got to meet other transgender people through the club scene and just sort of, you know, living, it was really late in college that I decided that, you know, I couldn't repress who I was anymore.
MARTIN: And how has it been?
COX: It's been a lot of things. I mean, I think the lives of women, in general, are complicated. It's been challenging on a lot of different levels. I've written and spoken a lot about street harassment, it's been one of the, probably, the more difficult parts of my experience. Violence against trans women is a very real part of the lives of so many trans women, particularly trans women of color. So, it's something that I often live in fear of. But through all the things, the difficulties of what it means to be who I am, I love myself and I'm so grateful that I get to live authentically now.
MARTIN: I wanted to ask, though, acting is a hard profession. It's the business of no. And then you add any other element - being a person of color, being a large-sized person, you know, whatever, that it's, OK, how am I going to find my role? How am I going to get parts. How did you start putting that together?
COX: It's been a very difficult road being an actor and being openly trans. It's been very, very difficult. And actually, for a while after I graduated, I thought, OK, I'm not going to be able to be an actor. But then I decided that acting is my passion and it's what I love most in the world. I've done a lot of independent films that folks haven't seen. I've done a lot of off-Broadway theater. So it's been a really rough and hard road. I think that is the case, as you said, for any actor - being trans, the roles that are written for us are very limited.
MARTIN: Well, yeah. To that point, I mean, just for people who have seen you in Netflix but they will have seen you before. You've played characters on "Law and Order," you were on the reality TV show "I Want to Work for Diddy," then you produced your own show on VH1, "TRANSform Me." You've mentioned that you played a prostitute seven times.
COX: Yes.
MARTIN: I wonder why that is?
COX: That is sort of the stereotype. That's the stereotype that when folks want to write a trans character, the first thing that they think of is sex work. And part of the reason is that the most visibility, really, that trans folk get is through sex work. And then there's also crazy unemployment rate among transgender people, it's like twice the national average. If you're a trans person of color, it's four times the national average. So, so often the only job opportunities presented for trans folks are in street economies including sex work. Obviously, there's lots of trans women who don't do sex work who have all kinds of professions.
MARTIN: If you're just joining us, we are speaking with Laverne Cox, a transgender actress who plays Sophia, a transgender inmate on the Netflix original series "Orange Is the New Black." With that background, then I think you can understand why this role as Sophia is a really important role for you. Sophia has a complicated family life. Years before she made her transition, she was a married man with a son. And I just want to play a short clip. In this scene, Sophia's wife is helping her choose some clothes. Here's a clip.
TANYA WRIGHT: (As Crystal Burset) Oh, my God.
COX: (As Sophia Burset) Good or bad?
WRIGHT: (As Crystal Burset) Not good. You look like Hannah Montana. That skirt is...
COX: (As Sophia Burset) I thought it was so cute. That's what happens when I shop for myself. It's 'cause I never got to be a teenage girl.
WRIGHT: (As Crystal Burset) And you never will be. You should be dressing like a classy, grown-up lady.
COX: (As Sophia Burset) You think I'm classy?
WRIGHT: (As Crystal Burset) I can't have my husband walking around like a $2 hooker.
MARTIN: That's a very beautiful scene, though. Don't you think?
COX: I love that scene, too, because it really illustrates how much these two people love each other. You know, I've said this before, that there's not a lot of black families on television like the Bursets, like Sophia and Crystal. And I love that we get to see this untraditional kind of black family on television.
MARTIN: And you have a twin brother, right?
COX: In real life, I have a twin brother.
MARTIN: In real life, who plays your character before the transition.
COX: Yes.
MARTIN: How handy is that? I mean, was that always envisioned that, I mean...
COX: ...Of course, when they cast me they didn't know that I had a twin brother, it's not something I, you know, say when I go to auditions. I initially wanted to play Sophia pre-transition, but Jodie Foster, who directed the episode, thought I looked too feminine to play the masculine firefighter that Marcus Burset was. They held auditions and my agent mentioned to the casting that I have a twin brother and he auditioned and the rest is history.
MARTIN: Isn't that something? What kind of reaction are you getting to Sophia?
COX: Different folks from the trans community have written me and said that they're so proud of this portrayal and they've been so profoundly moved. A beautiful blog was written about - by this woman who said that she didn't really, you know, approve of the trans gender thing and had all these misconceptions about trans people, and Sophia's storyline changed that for her.
MARTIN: Well, you know, it's interesting too, but the series also deals with the repulsion that some people have toward trans people. There's a scene, for example, in Season One where one of the other inmates is a...
COX: ...She's a sort of religious zealot.
MARTIN: She continues to refer to your character as, it.
TARYN MANNING: (As Tiffany 'Pennsatucky' Doggett) I don't want it here.
COX: (As Sophia Burset) Oh, please.
MANNING: (As Tiffany 'Pennsatucky' Doggett) That's why this whole thing happened. God's angry that there's an abomination in the church and if you don't get rid of it, it's just going to keep on happening.
PABLO SCHREIBER: (As George Mendez) All right. You listen to me...
MARTIN: You're an actor, so I'm not going to try to make you the spokes person for all people, but I have to say there are people who really believe that that is true. And I just want to ask how you deal with that.
COX: I think it's important, in terms of religion. I grew up religious. I was raised in the African Methodist Episcopal church and I still believe in God and I believe that God is about love. And I believe that it's not any of our jobs, as human beings, to judge other human beings. So I think whenever someone is having a problem with what another person is doing with their body or with their life, then it's usually because they have a problem with themselves.
MARTIN: Well, what's next?
COX: We're shooting Season 2 and we are - there's scripts and things coming in so we're looking at scripts and I have some speaking engagements coming up as well. I have a lot to say about a lot of things. So folks can, you know, find, you know, follow me on Twitter @LaverneCox.com
MARTIN: Maybe it's because social media, people have outlets to express their ideas in a way that they perhaps had not before. And recently, a number of actresses of color have expressed, you know, some unhappiness about the way they feel that they're limited in their choices.
COX: It's hard. I mean, I'm also a black actor, as well. So the issue of it's not just being trans, but it's being a woman, and it's being black. And the industry, historically, doesn't think that we're marketable or they want to cast us in very limited ways. But I think the wonderful lessons that "Orange Is the New Black" is teaching us is that it shows our industry, the entertainment industry, that you can cast women of different races, you can cast women of different ages and body types and folks will tune in and be interested and that the public is craving that.
MARTIN: Laverne Cox is featured on the Neflix original series "Orange Is the New Black." And she was kind enough to join us in our studios in New York. Laverne Cox, thank you so much for speaking with us.
COX: Thank you so much for having me. Transcript provided by NPR, Copyright NPR.

subset: commoncrawl


text:
Yahoo's CEO Marissa Mayer is one of 40 people up for Time's "Person of the Year."
She's up against some stiff competition. There's Sheldon Adelson, a conservative casino magnate. There's Ai Weiwei, China's most famous artist and dogged political dissident. There's Joe Biden, our veep.
And then, of course, there is Mayer, who made news in July when the Sunnyvale-based Yahoo announced it had hired the 37-year-old pregnant woman as its new CEO. She had been Google's 20th employee - working there for 13 years. And, many say, a key contributor to the Silicon Valley company's success.
Mayer's pregnancy - discovered hours after her new CEO gig was announced - fueled a debate in Silicon Valley on whether it was appropriate to sign on for a top executive gig so close to taking maternity leave. Mayer said she would not take a full leave. Instead she planned on a few weeks and would work from home during that time, too, she said.
She gave birth to baby Bogue weeks after taking the job, and she's now the Fortune 500's highest-profile working mom.
Mayer's description as No. 23 of Time's 38 candidates states that she had "yet to put her imprint on Yahoo ... but her mere presence has boosted its reputation."
"And the fact that she gave birth to a baby boy weeks after taking the job has made her the Fortune 500's highest-profile working mom," Time stated.
As always, the Time award brings fame and recognition, and even though viewers can cast their vote online, Time's editors choose the prestigious "Person of the Year" award. Voting closes at 11:59. p.m. on Dec. 12. The winner will be announced Dec. 14.


text:
Antitrust Case Filings
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text:
When I called the first Killers album "crappy," I was wrong (shoulda saved that opinion for their last two). When I guessed that 'Lost' was all in Hurley's head, I was wrong. When I said that no Ben & Jerry's flavor could ever top Phish Food, I was wrong (shout out to Americone Dream! Woot!) When I waited a few weeks to see "Batman Begins" because it couldn't be better than the Tim Burton Batman films, I was wrong. And when I said that you should drop Darko Milicic and never look back, I was wrong. I should have said you should drop him and constantly watch for both his confidence and his minutes to rise and for his two-step baby hook to develop. Or maybe I should have just told you to stash him on your bench. Mea culpa. What can I say? I'm only half-human. I won't be a full cyborg until they invent mechanical organs. Until they do, I'm just a human being with go-go-gadget limbs and the mistake-making part of me is still human.
The Minnesota center is averaging nearly 36 mpg in his last five along with 18/8.6/3.6 and 4.2 blocks. If this were Serge Ibaka or Kris Humphries or Chuck Hayes, many fantasy owners would be all over a guy producing like this. But he's owned in only about 60 percent of leagues and it's probably because the guy has been the punchline to tons of jokes that, frankly, were well worth a giggle lo these last few years. But Scrooge didn't just say he was sorry to everyone to whom he had been grumpy in the past. He bought them turkeys and presents and gave them tuppence and haypennies and other old timey money. So consider this my tuppence to Milicic. The guy's per36 numbers show that he's been within a rebound of averaging a double-double in three of his previous seven seasons. they also show that he's a blocking machine. Always has been. He's averaged 2.6 blocks per 36 his entire career. But until this year, he's never been given anything close to 36 minutes a game. He averaged 23 minutes twice, but his career average is 17. I can't jump aboard the Darko Milicic bandwagon after just five games. I won't. But I can kick the wagon's tires. Ask what kind of mileage this puppy gets. Remark that maybe, just maybe, it rides like a dream once it's one the road for a bit; after the driver shows confidence that it can reach its destination.* (*for the sake of this metaphor, I do feel compelled to note that one offshoot of never giving Darko much burn over the years is that he's still prone to excessive fouling, which was his downfall last night, and so, might not actually be able to reach his, um, "destination." But still … you get my point). I told you to drop Milicic because he's worthless. Now I'm telling everyone across all leagues to pick him up because he's not. I don't like doing it. I don't like thinking that perhaps David Kahn got one right. I'm not saying he did, I'm saying I'm considering the possibility that he did. Anyway, pick up Darko and apply him with care and patience.
Here's what else went down in a crazy fantasy basketball night:
Kevin Love – The guy dropped 32/22 last night, he's averaging over 20/14 on the season and I'm not sure he's even second in line on his own team for the NBA's Most Improved Player award.
Delonte West – Well, that was fun. Dude broke his wrist and will miss a handful of weeks. This isn't as big for West owners as it is for Nate Robinson owners, because until Rajon Rondo returns (maybe Friday, maybe not) Sugar Nate and some schmoo named Avery Bradley are all the floor generals the C's have left. No, don't bother picking up Bradley. Yes, continue holding onto Robinson until Rondo returns.
Shaquille O'Neal – 25/11 in 22 mpg. Well hell, if you're excited about owning Milicic, this must be November 2003. And if it's 2003, why wouldn't you own Shaquille O'Neal?
Tyrus Thomas – It's a Thanksgiving surprise from Larry Brown! Be thankful for his giving Tyrus Thomas the starting center spot in which he dropped 26/11 and four blocks on the Knicks. And be thankful for T-Time giving a damn about making the opportunity count. Both are extremely rare occurrences. Now be thankful that it lasts the rest of the season. What? We can't preemptively be thankful for things?
Jose Calderon – Shot 70 percent from the floor after I predicted his ceiling was somewhere around 13/6. I ask you, what's more likely? That I'm wrong or that Calderon makes nearly three out of every four shots he takes from here on out?
Reggie Evans – Grabbed a career-best 22 rebounds to go along with a surprising 12 points. "Oh, that's adorable." – Kevin Love.
Evan Turner – The no.2 overall pick blew tonight (2/2/0 in 16 minutes), but he's been averaging a respectable 10/6.5/2 as a starter, despite shooting horrifically. Considering he doesn't have a horrific shot, I'd suggest being patient with him.
Brandon Jennings – He shot 10 percent from the floor and ended with three points and four assists. After starting out with a hot assist hand (8.5 in his first four games) he's become Cool Hand Brandon (4.6 in his last 10).
J.J. Hickson – Two points. Five boards. Still starting over Antawn Jamison. Jamison, for his part, didn't do much better, like when subjects before a king lower their head so as not to be higher than the crown. Cleveland reserves must play just slightly below the king. No, not that 'King.' Those jokes are, like, so summer 2010.
J.J. Redick – To stay with the J.J. theme, Redick took over for the injured Vince Carter and dropped 20/4/5 in 43 minutes. Also dropped? All the gunk he used to put in his hair. Now Marcin Gortat doesn't hate playfully mussing his hair after every big three-pointer.
Brandon Bass – Career-high 18 points in 26 minutes. That's a start but let this Bass bite on this line a few more times before reeling him in.
O.J. Mayo – Benchslapped! But not by any owners this time, by head coach Lionel Hollins. He went 9/1/2 in 23 minutes off the bench, but this smells more like a wake-up call to O.J. than a serious shift. Apparently Hollins just likes his lineup with no Mayo. Relax guys, George Tomato'n'onions is still in the clear.
Richard Hamilton – Ejected for the second time in a week. Meet the new 'Sheed, same as the old 'Sheed … except the old 'Sheed got tossed for being angry at the refs or other players. The new 'Sheed is getting tossed for the same reason employees bring a paperback book into the office bathroom for 20 minutes: because they can no longer stand the mind-numbing awfulness of their job.
Jason Maxiell – Charlie V played only 22 minutes so Maxiell could play 23. … I'm not going to write about the Pistons anymore this year.
James Jones – 0-for-5 from the floor (all from beyond the arc). Drop this threejerk like he's cold.
Tyson Chandler – Season highs in points (17) and rebas (18) after Brendan Haywood went down with a leg injury. Haywood who? Hey would you stay on the bench so I can grab another seven or eight rebounds? 'Kaythanks.
Kyle Lowry – He's averaging 12.7/10 in his last three games and has looked as comfortable as anyone on the forever-shaky Rockets can look.
Chuck Hayes – 16/7/5 He played almost 29 minutes. It's worth noting Brad Miller and Jordan Hill played a combined 7:37. It's because Miller and Hill are slow and the Warriors are not.
Dorell Wright – Played all but three minutes and filled up every part of the box score with happy numbers.
Hakim Warrick – In case you were still weighing the worth of Warrick versus Turkoglu, Warrick played two more minutes and scored 13 additional points.
Grant Hill – Scored a season high 25 points. And even though it was a double overtime game and those tend to produce these kinds of numbers, Hill has scored 21+ points in three of his last four and is owned in fewer than half the leagues out there. C'mon, people!
Kyle Korver – 24/5/3 in over 47 minutes in this double-overtime doozey. He won't get this many kind of burn again any time soon. In fact, he's only seen 47+ minutes one other time in the last five seasons.
Derrick Rose – I don't really need to comment on Rose's 35/12/7 game. You already own him. Or wish you owned him. Or wish the guy you're going head-to-head against didn't own him. But wishin' don't make it so. If it did, I'd have already seen my Ty Lawson – Rondo – Rose footrace Olympiad long ago.


text:
Good 60 Minutes piece on DHL & Unemployment in Wilmington. It illustrates the problems and difficulties of unemployment and underemployment...
A Town in Crisis (13:23)
Watch CBS Videos Online
Friday: Personal Income and Outlays
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World View & Market Commentary.
Forest first; Trees second.
Focused on Real & Knowable facts that filter through the "experts" fluff and media hyperbole. Where we've been, what the future may hold and developing a better way forward.


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Holland currently benefits from a stable housing market which is why it is so attractive to people seeking long-term investments. Buying a holiday home in any country comes with a certain amount of risk, meaning even holiday homes in the Netherlands need protecting.
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The village of Taybeh near Ramallah in the occupied West Bank held its first-ever wine festival last weekend.
Hundreds of people from around the West Bank, Jerusalem and present day Israel converged on the small village on Friday and Saturday evening to sample the wine, to eat and to hear some local Palestinian bands.
The Palestine Monitor spoke over the phone to Madees Khoury, who works at the brewery and winery in Taybeh. "The event was very successful, it was a busy weekend, a lot of people came and I think people really enjoyed the wine… We hope it's going to be an annual event," she said.
The Taybeh winery was established in August 2013 and has been selling red Merlot and Syrah wines throughout the West Bank since. During last weekend's "Vinfest," they launched their new white Sauvignon Blanc.
The village is well known throughout the Middle East, not only for its historical significance but also due to the brewery that was founded there in 1994 by Nadim Khoury. Taybeh brewery was the first microbrewery in the Middle East, and since its inception, the beers have won international acclaim. Taybeh beer is a firm customer favourite throughout the West Bank and present day Israel.
Nadim's son, Canaan, recently returned from studying mechanical engineering and material science at Harvard University, and decided that he wanted to expand the family business into wine production. Having received training from an Italian winemaker, Canaan crushed the first red grapes from nearby Aboud and Birzeit to make wine in August 2013. With the success of the red, he began experimenting with white grapes, and eventually began bottling the new Sauvignon Blanc in early 2015.
Madees explained that the successful expansion of the business has not been without obstacles. "Obviously it's not easy doing business under occupation… There are many difficulties and restrictions so it's not easy but it's not impossible either," she said. For example, seen as Palestine doesn't have a port or an airport, all exports must pass through Israeli checkpoints, being unloaded and reloaded which brings many additional costs and takes more time, making it extremely difficult for Palestinian produce to compete in international markets.
Madees said that the family's positive attitude is what has helped them overcome the difficulties and successfully grow the business. "You can't keep blaming the occupation, you can't let it stop you. You have to be determined", she outlined.
The family have many plans to continue to expand the business and build on their success. "They [Madees' father Nadim and brother Canaan] have high hopes for a better future and a peaceful future. We all have high hopes for the business, Insha'Allah."
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Top Items:
Rachel Donadio / New York Times:
Frail Pope Breaks Tradition and Resigns — VATICAN CITY — Citing advanced years and infirmity, but showing characteristic tough-mindedness and unpredictability, Pope Benedict XVI shocked Roman Catholics on Monday by saying that he would resign on Feb. 28, becoming the first pope to do so in six centuries.
Discussion: alan.com, Reuters, Fox News, protein wisdom, Wall Street Journal, Telegraph, Guardian, News Desk, The Week, Hot Air, American Spectator, Business Insider, Advocate, americanthinker.com, Politico, Taylor Marsh, Quartz, No More Mister Nice Blog, Outside the Beltway, Achenblog, Mother Jones, Mock, Paper, Scissors, Hugh Hewitt's TownHall Blog, The Confluence, Shakesville, The Spectacle Blog and Lawyers, Guns & Money
Philip Pullella / Reuters: Pope Benedict surprises world, steps down citing frailty
Jeff G. / protein wisdom: Pope Benedict XVI to resign
Alessandra Galloni / Wall Street Journal: Pope Benedict to Resign
Jennifer O'Mahony / Telegraph: Pope resigns: live
Amy Davidson / News Desk: Pope Benedict's Resignation and John Paul's Suffering
Matt Purple / American Spectator: So Long, Benedict — He was only to be an interim pope; a placeholder.
Michael Brendan Dougherty / Business Insider: One Of These Men Will Be The Next Pope
Michelle Garcia / Advocate: Pope Benedict XVI Announces Resignation
Rick Moran / americanthinker.com: Pope Benedict in shock resignation
Katie Glueck / Politico: Pope Benedict XVI's politics with Bush, Obama
Lily Kuo / Quartz: Five facts about popes
Steve M. / No More Mister Nice Blog: REMIND ME AGAIN: WHY IS THIS THE BIGGEST NEWS STORY IN AMERICA?
James Joyner / Outside the Beltway: Why Pope Benedict is Resigning
Joel Achenbach / Achenblog: Pope Benedict XVI calls it quits
Kevin Drum / Mother Jones: Here's Hoping for a Better Pope
Hugh Hewitt / Hugh Hewitt's TownHall Blog: On Benedict and His Successor
Riverdaughter / The Confluence: The Pope Thing — Frankly, I'm surprised that more popes haven't resigned.
Melissa McEwan / Shakesville: The Pope Is Resigning — Welp, looks like the Pope is putting …
Wlady Pleszczynski / The Spectacle Blog: Pope Benedict to Resign
Robert Farley / Lawyers, Guns & Money: I Say "Back to Avignon!"
Igor Volsky / ThinkProgress:
What Did Pope Benedict Do, Anyway? — Pope Benedict XVI, who took office in 2005, announced on Monday that he will resign on Feb. 28, the first pope to leave the papacy since Pope Gregory XII in 1415. The 85-year-old pontiff is in poor health and has been advised by doctors "not to take any more transatlantic trips."
Discussion: NBCNews.com, msnbc.com, NewsHounds blog, ProfessorBainbridge.com, NPR, Law Blog, Mashable! and FIRST DRAFT
Aliyah Frumin / msnbc.com: Pope Benedict retires: What will his legacy be?
Priscilla / NewsHounds blog: Martha MaCallum & Fox VP Ignore Pedophilia Scandal During Papal Lovefest
Steve Bainbridge / ProfessorBainbridge.com: Pope Benedict to Resign
Scott Neuman / NPR: Pope Benedict Leaves Behind A Mixed Legacy
Jacob Gershman / Law Blog: A Legal Guide To Papal Resignation
Stan Schroeder / Mashable!: Pope Benedict XVI to Resign on February 28
Athenae / FIRST DRAFT: Does He See the RICO Case Coming?
David Willey / BBC:
Pope Benedict XVI — Born in Germany in 1927, joined Hitler Youth during WWII and was conscripted as an anti-aircraft gunner but deserted — As Cardinal Joseph Ratzinger spent 24 years in charge of Congregation for the Doctrine of the Faith - once known as the Holy Office of the Inquisition
Discussion: The Moderate Voice, Reuters, Twitchy, The Week, Klavan On The Culture, Da Tech Guy's Blog, The Anchoress, The Daily Beast, Agence France Presse, Telegraph, Guardian, The PJ Tatler, NBCNews.com, The Independent, Little Green Footballs, The Other McCain, Scared Monkeys and Red Alert Politics
Patrick Edaburn / The Moderate Voice: Papal Frontrunners Include Two Africans And One Canadian
Philip Pullella / Reuters: Pope's sudden resignation sends shockwaves through Church
Lori Ziganto / Twitchy: Pope Benedict XVI resigns and moral cretins tweet hate, death wishes; Resign by 'slitting your wrists'
Andrew Klavan / Klavan On The Culture: Turn Out the Lights of Europe When You Leave, Pope!
Elizabeth Scalia / The Anchoress: Benedict Announces Retirement (It's what he WOULD do!) UPDATES!
Michael Tomasky / The Daily Beast: An Opportunity for the Catholic Church
Katherine Haddon / Agence France Presse: Pope Benedict XVI's resignation: Live Report
Alex Spillius / Telegraph: Pope Benedict XVI's successor: Africans and Latin Americans among early contenders
Scott Ott / The PJ Tatler: Piers Morgan Tweet Sparks Papal Confession
The Independent: Benedict XVI is first Pope to resign in six centuries - and only the fifth ever to stand down
Robert Stacy McCain / The Other McCain: POPE BENEDICT XVI RESIGNS
Scared Monkeys: Pope Benedict XVI Announces He Will Resign at the End of February ... First Papal Resignation in 600 Years
Sarah Muro / Red Alert Politics: Pope Benedict XVI in shock resignation
Pope Benedict XVI announces his resignation at end of month — Pope Benedict XVI on Monday said he plans on resigning the papal office on February 28th. Below please find his announcement. — Full text of Pope's declaration — Dear Brothers, — I have convoked you to this Consistory …
Aaron Blake / The Fix: How they elect the pope (VIDEO)
Ed Morrissey / Hot Air: Breaking: Pope Benedict resigning at end of month
John Stanton / BuzzFeed: Catholic Left Looks For An Opening With A New Pope
Max Fisher / WorldViews:
The bizarre stories of the four other popes to have resigned in the last 1,000 years
The bizarre stories of the four other popes to have resigned in the last 1,000 years
Discussion: Guardian, Poynter, Hit & Run and FIRST DRAFT
Andrew Beaujon / Poynter: Pope Benedict XVI resigns: What you need to know
Jesse Walker / Hit & Run: The Ones Who Walk Away from the Holy See
Kevin Robillard / Politico:
Pope Benedict XVI: Politicians praise ahead of resignation
Pope Benedict XVI: Politicians praise ahead of resignation
Discussion: Reuters, CNN and Associated Press
Duck of Minerva:
What Political Science Says About Papal Elections
What Political Science Says About Papal Elections
Discussion: Washington Monthly, Washington Post, Spectator, The Dish, Daniel W. Drezner, Taegan Goddard's …, Blazing Cat Fur and National Review
Ed Kilgore / Washington Monthly: Early Papal Line
Dylan Matthews / Washington Post: The political science of papal elections
Freddy Gray / Spectator: A New York Pope? Why I'm betting on Cardinal Dolan
Andrew Sullivan / The Dish: The Pope Resigns: Reax
USA Today:
Pope Benedict XVI to resign
Pope Benedict XVI to resign
Discussion: The Reaction, The Awl, The Agonist and The Latest Word
Carl / The Reaction: Pope Benedict to resign
Ken Layne / The Awl: Farewell To Our Beloved Nazi Pope
Numerian / The Agonist: Why Benedict XVI Quit
Michael Roberts / The Latest Word: Photos: Pope Benedict resignation memes, Twitter Nation reaction
Can a pope resign? — Yes, a pope can resign — up to 10 popes …
Can a pope resign? — Yes, a pope can resign — up to 10 popes …
Discussion: The Dish, Firedoglake and NPR
Andrew Sullivan / The Dish: The Pope Resigns: Your Thoughts
Mark Memmott / NPR: Pope Benedict XVI Is Resigning
The unprecedented resignation of Benedict
The unprecedented resignation of Benedict
Discussion: Washington Monthly
Ed Kilgore / Washington Monthly: Ratzinger's Strange Reign
Washington Post:
Pope Benedict XVI to resign, citing age and waning energy
Pope Benedict XVI to resign, citing age and waning energy
Discussion: The Fix, Guardian, The Moderate Voice, WorldViews and The Daily Caller
Chris Cillizza / The Fix: What the Catholic vote tells us about presidential elections
Jon Henley / Guardian: Can the pope resign?
Phil Bronstein / Esquire:
The Man Who Killed Osama bin Laden... Is Screwed — For the first time, the Navy SEAL who killed Osama bin Laden tells his story — speaking not just about the raid and the three shots that changed history, but about the personal aftermath for himself and his family.
Joe Gandelman / The Moderate Voice: Plight of the Shooter Who Killed Osama bin Laden
Libby Spencer / The Impolitic: To serve with honor and retire with nothing
Larry Johnson / NO QUARTER USA NET: Crocodile Tears for a Foolish SEAL?
John Aravosis / AMERICAblog: I didn't kill bin Laden, and I have to find my own health insurance & pension too
Arturo Garcia / The Raw Story: Esquire reporter: Man who killed bin Laden faced life 'like a mafia snitch' after leaving Navy
Kate Seamons / Newser: I Killed Osama bin Laden— and Now I'm Unemployed
Kevin Cirilli / Politico: Report: Navy SEAL kills Osama bin Laden, loses health care
Meenal Vamburkar / Mediaite: Navy SEAL Who Says He Killed Bin Laden Speaks Out For First Time, Says Military Abandoned Him
Jim Hoft / The Gateway Pundit: Tragic... Navy SEAL Who Killed Bin Laden Is Jobless, Broke & Without Security
Caroline May / The Daily Caller: Report: SEAL Team 6 member who killed bin Laden left with a 'go f*** yourself' from government
Jammie Wearing Fools: SEAL Team 6 Commando Who Shot bin Laden Speaks: 'This is real and that's him. Holy s**t'
Meghashyam Mali / The Hill:
Sen. Inhofe threatens filibuster to block Hagel — Sen. James Inhofe (R-Okla.) on Sunday threatened to filibuster former Sen. Chuck Hagel (R-Neb.)'s nomination for Defense secretary, if necessary to prevent his confirmation. — "I want a 60-vote margin and you don't have to filibuster to get that …
Discussion: Daily Kos, The Maddow Blog, americanthinker.com, The Right Scoop, Israel Matzav and Mock, Paper, Scissors
Steve Benen / The Maddow Blog: Graham: 'No confirmation without information'
The Right Scoop: Lindsey Graham to delay confirmation of nominees to force White House to come clean on Benghazi
Tim Mak / Politico:
GOP threatens walkout on Chuck Hagel vote
GOP threatens walkout on Chuck Hagel vote
Discussion: Washington Monthly, Israel Matzav, The Week, Taylor Marsh and Lawfare
Ed Kilgore / Washington Monthly: Graham's Continuing Hissy Fit
Taylor Marsh: Republicans Threaten Hagel Walkout & Filibuster, While Levin Letter Reveals Their Hypocrisy
John Bellinger / Lawfare: Senator Graham's Threat to Block Hagel/Brennan Nominations
Jeremy Herb / The Hill:
Armed Services panel to vote Tuesday on Hagel confirmation
Armed Services panel to vote Tuesday on Hagel confirmation
Discussion: CBS News and National Review
Major Garrett / CBS News: Hagel confirmation votes to be held this week
Glenn Thrush / Politico:
Obama's State of the Union: Aggressive — President Barack Obama's State of the Union speech will be less a presidential olive branch than a congressional cattle prod. — Emboldened by electoral victory and convinced the GOP is unwilling to cut deals, Obama plans to use his big prime …
Discussion: CNN, Washington Post, NBCNews.com, USA Today, American Prospect, Angry Bear, The Plum Line, First Read, Hot Air, The Huffington Post, msnbc.com and The Hill
Zachary A. Goldfarb / Washington Post: Obama weighing executive actions on housing, gays and other issues
First Read / NBCNews.com: Obama returns to a focus on the economy
Susan Page / USA Today: State of the Union: Obama's audience of four
Paul Waldman / American Prospect: Tomorrow's Republican Post-SOTU Whining Today
Beverly Mann / Angry Bear: The People-as-Props Who Obama SHOULD Use During his Speech Tomorrow Night: John Boehner and Joe Barton.
Jamelle Bouie / The Plum Line: Why Obama should ignore the deficit in his SOTU
Robert Kuttner / The Huffington Post: The Sorry State of Our Union
Court Harson / msnbc.com: Let Me Start: State of the GOP's Union
Quinnipiac News + Events:
Release Detail — February 11, 2013 - Obama Should Focus State …
Release Detail — February 11, 2013 - Obama Should Focus State …
Discussion: The Hill, msnbc.com and Business Insider
Jonathan Easley / The Hill: Poll: Voters trust Obama over GOP on economy ahead of State of the Union
Morgan Whitaker / msnbc.com: Obama better trusted on economy than GOP
Ben Smith / BuzzFeed:
Obama Prepares To Screw His Base — Young people re-elected the president. Now they get to pay disproportionately for ObamaCare. — President Obama talks with college students while waiting for an order of fries at OMG Burgers in Coral Gables, Florida last September. — Image by C.W. Griffin/Miami Herald/MCT
Discussion: Wonkblog, The Mahablog, FreedomWorks blogs, Le·gal In·sur·rec· tion, BizzyBlog, Taylor Marsh, Right Wing News and Balloon Juice
Sarah Kliff / Wonkblog: Is Obamacare bad for young people? Let's ask them!
Kristina Ribali / FreedomWorks blogs: Obamacare's Biggest Loser: The Youth
William A. Jacobson / Le·gal In·sur·rec· tion: Bwahaha — Now they tell them! … We've been pointing that out forever.
Tom / BizzyBlog: Buzzfeed's Ben Smith: 'Obama Prepares To Screw His (Young) Base' — As If It Hasn't Been Happening for Years
William Teach / Right Wing News: Oh, Snap: "Obama Prepares To Screw His Base"
Josh Marshall / Talking Points Memo:
Don't Believe the Hype — Let's just say it: Marco Rubio is the Wes Clark of 2013. Only with many fewer accomplishments. — It pains me to say this because I'm an admirer of Gen. Wesley Clark. I think he would have made a good president. He was an extremely accomplished career military officer.
Discussion: The Daily Caller, Booman Tribune and The Raw Story
Matt K. Lewis / The Daily Caller: Why Marco Rubio isn't 'the Wes Clark of 2013′
Jonathan Terbush / The Raw Story: GOP strategist: We need Rubio because 'he knows who Tupac is'
Nate Silver / FiveThirtyEight:
New Rove Group Could Backfire on G.O.P. — The strategist Karl Rove and his allies last week announced the formation of Conservative Victory Project, a new "super PAC" designed to lend support to what they see as more electable candidates in Republican Senate primaries.
Discussion: Washington Monthly and Prairie Weather
Ed Kilgore / Washington Monthly: Who's Afraid of Karl Rove?
Annie-Rose Strasser / ThinkProgress:
Virginia Cuts State Employees' Hours To Avoid Providing Obamacare Coverage — As part of his state's new budget, Virginia Gov. Bob McDonnell (R) and his administration are trying to force potentially tens of thousands of public sector employees in the state to work fewer hours so that the government …
Discussion: ECHIDNE OF THE SNAKES, Outside the Beltway, Mock, Paper, Scissors and Balloon Juice
Tim F. / Balloon Juice: Best healthcare in the world
Wall Street Journal:
The Absentee Commander in Chief — The Defense secretary told the president that Americans in Benghazi were under attack. Then: nothing. — We've both had the honor to work in the White House. We've seen presidents, vice presidents, chiefs of staff and national security advisers during moments of international crisis.
Discussion: Althouse, americanthinker.com and msnbc.com
Rick Moran / americanthinker.com: 'The Absentee Commander in Chief'
Rick Bosh / msnbc.com: What we're reading: Monday, February 11, 2013
Scott Keyes / ThinkProgress:
NRA Dismisses 'Connecticut Effect,' Suggests Grief Over Newtown Tragedy Will Be Short-Lived — WAUSAU, Wisconsin—The National Rifle Association will wait until the "Connecticut effect" has subsided to resume its push to weaken the nation's gun laws, according to a top NRA lobbyist speaking …
Discussion: The Raw Story and Daily Kos
David Edwards / The Raw Story: NRA lobbyist: Pro-gun laws delayed over temporary 'Connecticut effect'
Barbara Morrill / Daily Kos: The NRA has a name for 20 dead children: the 'Connecticut effect'
Neil Irwin / Washington Post:
Is Congress really going to miss its free lunch on infrastructure? — Since the election, there have been hints that we could be entering a period with some actual productive, bipartisan dealmaking, most explicitly on immigration reform. But the Republican reaction to Obama's expected proposals …
Discussion: No More Mister Nice Blog and Mother Jones


text:
(HealthDay) -- The United States needs to spend more on its chronically underfunded public health system and use that money more efficiently, according to an Institute of Medicine report released Tuesday.
The United States spends more on health than any other nation -- nearly $2.5 trillion in 2009 -- but has lower scores on life expectancy, infant survival and other indicators of population health than other wealthy nations, according to the report.
Public health department initiatives, services and expertise can help prevent or decrease rates of chronic diseases that account for the bulk of rising U.S. health spending, the authors report.
However, only 3.1 percent of U.S. health spending went to government-administered public health in 2009, according to the U.S. Centers for Medicare and Medicaid's National Health Expenditure Accounts. That works out to $251 per person in public health spending, compared with $8,086 per person in medical care spending.
The report calls for the U.S. Department of Health and Human Services to establish new goals for life expectancy and per-person health spending. The hope is that setting targets will motivate public health and medical care professionals to work together to maximize the value of health spending, and that public health skills and knowledge are used to address some of the biggest issues facing the larger health care system, such as the unnecessary use of medical procedures.
To achieve efficient use of public health dollars, the report recommended that the U.S. National Prevention, Health Promotion, and Public Health Council -- created by the Affordable Care Act -- should establish the minimum level of public health services every community should receive from its state and local health departments.
In addition, the council should create an expert panel to determine how much money is needed for every public health department to provide at least these minimum services, and to determine the proportion of federal health spending that needs to be spent in public health and medical care in order to get better value, the report said.
"Developing and implementing strategic population-based efforts to improve our health as a nation will increase the quality of life and productivity of Americans at the same time that it will contribute to moderating the expense of the clinical care system," report committee chair Dr. Marthe Gold, a professor and chair of the department of community health and social medicine at City College of New York, said in a National Academies news release.
"The country's failure to maximize the conditions in which people can be healthy continues to take a growing toll on the economy and on society. As the backbone of the health system, public health departments could help communities and other partners engage in efforts and policies that lead to better population health," Gold said.
More information: The U.S. Department of Health and Human Services has more about public health service agencies.
Copyright © 2012 HealthDay. All rights reserved.


text:
#RSL Tweets of the Day (@RealSaltLake)
#RSL acquires FW @JoaoPlata10 from Toronto FC in exchange for 2015 2nd Round Pick http://instagr.am/p/VHRlGysFR-/
Big welcome to our newest wee-man, Joao Plata! Very excited ab this add - extremely crafty, quick, and dangerous player. Here we come!
Gracias dios por todo ,Thank God for everything #godislove
ICYMI. Fabulous insight into preseason life by Cole Grossman:http://kirsoccer.wordpress.com/2013/01/29/postcards-from-camp-cole-grossman/ … #RSL #MLS #preseason#realsaltlake
#RSL men @KyleBeckerman (74') and @tonybeltran23 (45') with starts tonight for #USMNT v Canada ... Congrats boys! #RSLfam
Jonah Freedman @jonahfreedman
Want to see a textbook definition of what "box-to-box midfielder" is? Check out @KyleBeckerman's heat map from #USAvCANhttp://www.mlssoccer.com/matchcenter/2013-01-29-USA-v-CAN/chalkboard …
I wish haha"@pabloricchetti: Morales comes on for Beckerman, it's his first cap for the #USMNT too.#USAvCAN, 76'"/Felicidades@javi_mo11
ESPN 700: A conversation with RSL owner Dell Loy Hansen
ESPN 700: Nick Rimando joins "The Bill and Hans Show"
USsoccer.com: MNT vs. Canada: Highlights – Jan. 29, 2013
Real Salt Lake News & Notes
RealSaltLake.com: Real Salt Lake acquires FW Joao Plata from Toronto
RealSaltLake.com: Beckerman, Beltran start in US's 0-0 draw v. Canada
The Salt Lake Tribune: Real Salt Lake acquires forward Joao Plata from Toronto FC
OK Español: Real finalize primera concentracion
For the Fans, By the Fans
RSL Soapbox: RSL acquires forward Plata in exchange for 2015 draft pick
RSL Soapbox: Beckerman captains USMNT for first time; Beltran starts for first cap
Keepin' it Real: Postcards from Cap: Cole Grossman
MLS, Other News & Notes
MLSsoccer.com: Recap: Canada, USA play to drab 0-0 stalemate in Houston
MLSsoccer.com: Davis on role with national team: "I know I fit at this level"
MLSsoccer.com: Klinsmann prefers conclusions from camp, not drab draw
MLSsoccer.com: De Rosario basks in love of Houston return in Canada draw
MLSsoccer.com: BBC: Sporting KC's Kamara heading to Norwich City
MLSsoccer.com: Stoke City boss Pulis says Shea transfer deal is close
MLSsoccer.com: US striker Altidore target of racist chants in Netherlands
MLSsoccer.com: Red Bulls confirm Fraser will join revamped coaching staff
SI.com: U.S. fails to impress in 0-0 friendly against Canada
SI.com: U.S. talent pool looks more like puddle in 0-0 draw
SI.com: Player ratings: Few U.S. players shine against Canada
American Soccer Now: Inept U.S. "B" team fails to create against Canada
American Soccer Now: Takeaways from the U.S.'s limp draw against Canada
American Soccer Now: A disappointing match, but some beautiful images
Soccer By Ives: USMNT and Canada finished scoreless yet again
Soccer By Ives: USMNT 0, Canada 0: A look back
Soccer By Ives: Gatt flashes speed, promise as one of few bright spots for USMNT v. Canada
Soccer By Ives: Report: Kamara poised for transfer to Norwich City
Pro Soccer Talk: Opportunities lost: Too few sharp edges for U.S. national team
Pro Soccer Talk: Good performances hard to find in U.S. draw with Canada
Pro Soccer Talk: Would Mix Diskerud have been worth a Designated Player slot? Merritt Paulson has thoughts
Pro Soccer Talk: Kei Kamara looks set for Premier League move
Pro Soccer Talk: Jozy Altidore's poised response to Tuesday's ugliness
Goal.com: United States 0-0 Canada: Americans put on ugly showing against inexperienced Canadians
Goal.com: Player ratings: USA 0-0 Canada
Goal.com: Five players make U.S. debuts in draw with Canada
Fox Soccer: USA ties Canada ahead of qualifier