text:
MMRF, TGen to lead multiple myeloma study
May 2012
NORWALK, Conn.The Multiple Myeloma Research Foundation (MMRF) recently announced a multi-year oncology research partnership with several partners including the Translational Genomics Research Institute (TGen), Spectrum Health and the Van Andel Research Institute (VARI). The collaboration will provide a broad range of genomic services and analyses to help drive the success of a landmark 1,000-patient study on the molecular segments and variation of multiple myeloma which has been launched by the MMRF.
Under the agreement, TGen will provide a central hub where patient samples will be analyzed using an unprecedented breadth of genomics platforms. The data to emerge from this work will provide the most comprehensive view of myeloma at the molecular level and will enable the research community to better understand what drives a patient's response to treatment or disease progression and will also generate new leads for targeted drug development.
The MMRF began enrolling patients in the landmark study Relating Clinical Outcomes in MM to Personal Assessment of Genetic Profileknown as CoMMpasslast year through a network of academic and community clinical centers. Study participants will provide an initial tissue sample at the time at which they are newly diagnosed, and will provide follow-up tissue samples at the time of first and additional relapse. Sequential analysis of these tissue samples will shed new light on the relationship between molecular variation and patients' response or resistance to therapy.
"We are proud to support the MMRF's unparalleled research initiative, which has tremendous potential to make a significant difference in the way multiple myeloma is treated," said Dr. John Carpten, professor and director of TGen's Integrated Cancer Genomics division, in a statement. "The rigor and breadth of this effort will enable a much more sophisticated understanding of the molecular changes that give rise to myeloma, and that affect a patient's course of disease. We believe the collective data to emerge along the way will provide an invaluable resource for innovators to design the next significant breakthroughs against this incurable disease."
Analyses during the study will also apply and build on insights from the Multiple Myeloma Genomics Initiative (MMGI) sequencing project to identify specific subgroups of multiple myeloma patients. For example, the study will involve sequencing tissue samples using techniques perfected in the MMGI and will test for mutations activating the BRAF gene, which were found in a small percentage of multiple myeloma patients in the sequencing project.
"We are excited to build on our earlier partnership with TGen. Their dedicated commitment to multiple myeloma genomic research and earlier achievements will play a critical role in the success of this initiative," said Dr. Louise M. Perkins, chief scientific officer at the MMRF. "The strong collaboration of academia, the clinical community and industry in this landmark project will enable us to translate new information into improved treatment approaches more efficiently and effectively than ever before."
TGen will be working with VARI to centrally collect and store tissue samples and extract DNA and RNA from samples for next-generation sequencing analysis including whole-genome and RNA-sequencing. Using the Program for Biospecimen Science and its biorepository at VARI, Dr. Scott Jewell's program will use collection and biobanking best practices to centrally manage the collection and biobanking for this study. VARI will process the specimens, isolate the cancer cell population and prepare derivatives for genomic analysis at TGen. VARI will use the VARI/TGen bioinventory software to assist in the tracking and management of the biospecimens throughout the life of the project.
Spectrum Health, which is accredited by the College of American Pathology and is also a Clinical Laboratory Improvement Amendments (CLIA) laboratory, will provide the BRAF gene analysis and a flow cytometric immunophenotype of each patient's myeloma using state-of-the art analytics in its clinical diagnostics laboratory.
"We are very excited to be a partner in this effort to determine how to conquer this disease," said Lisa A. Shannon, chief operating officer of Spectrum Health Grand Rapids. "This is very important research and we are proud to lend our expertise and experience to the effort. Partnerships like this expand the opportunity for success in conquering diseases like multiple myeloma."
This study is currently enrolling patients at clinical centers throughout the United States.

subset: commoncrawl


text:
In October 2010, Melanie Flintoft of Australian Fashion Labels launched Keepsake, a collection of on-trend, affordable eveningwear. Keepsake clothing features bold lines and silhouettes that meet classic fabrics and colors. The label launches new collections frequently, incorporating quality fabrics, beautiful prints, and unique shapes for a look that is singularly feminine. Favored by fashionable celebrities, Keepsake apparel has grown from its Australian roots into a worldwide presence.
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text:
Recipe from Bob Gallahan of Clifton Forge, Va.
Prep Time: 30 min
Total Time: 1 hour
2 cups vinegar
8 T worcestershire sauce
4 oz ketchup
6 T A-1 Steak Sauce
3 tsp salt
10 T brown sugar
2 tsp dry mustard or 1 T yellow mustard
1 T paprika
2 T crushed red pepper
2 T fresh lime juice
1/4 cup honey
1/4 cup molasses
Mix ingredients, pour in sauce pan and simmer for 30 minutes.
Yield: 2 1/2 cups
Comments: A great pork sauce
*DISCLAIMER*: The information contained in or provided through this site section is intended for general consumer understanding and education only and is not intended to be and is not a substitute for professional advice. Use of this site section and any information contained on or provided through this site section is at your own risk and any information contained on or provided through this site section is provided on an "as is" basis without any representations or warranties.


text:
MONTREAL - Not a second goes by that Dana Quigley isn't thinking of his son Devon – even when he's on the golf course.
"I think about him every moment of every single day," Quigley said. "I play golf every day, but the whole time I'm out there I think of him and I pray."
Quigley's 27-year-old son has been in a coma-like condition in a Florida hospital since he was seriously injured in a car crash the night of Nov. 30 while on his way home from a charity golf tournament.
Devon's car slammed into the rear of a tractor trailer. He suffered massive head trauma, was placed in a medically induced coma and remained in critical condition in intensive care in the St. Mary's Medical Centre for more than a week.
Devon is no longer in an induced coma, but remains in a coma-like state. He has shown signs of recovery, albeit gradual, over the last couple of months. He's still unable to communicate verbally, but a few weeks ago started moving his legs for the first time.
"The doctors have called (Devon's condition) all kinds of stuff, but we don't know exactly what is happening," Quigley told The Gazette from the hospital, days before his son began to show movement in his legs.
"He responds to questions … just with his eyes … the only thing he moves. He seems to be coming 'round, but it is a very slow process," the Champions Tour veteran added. "We all just have to continue to pray for him."
Quigley turned 65 on April 14. He became the Champions Tour's iron man when he played a record 278 consecutive tournaments from 1997 to 2005. He missed only one of the first seven tournaments this season and plans to play 15-20 in all, but stressed that "everything depends on Devon."
Quigley expects to be in town for the third edition of the Montreal Championship presented by Desjardins, slated for June 22-24 at the Richelieu Valley Golf Club in South Shore Ste. Julie. He tied for 47th in last year's event at Le Fontainebleau in Blainville after missing the inaugural edition the year before.
"I'm looking forward to coming back to Montreal," Quigley said. "It was a great tournament last year. The tournament director (David Skitt) did a fabulous job of taking care of us, making us feel at home. It's one of the best run tournaments we have."
Quigley will return to Richelieu Valley for the first time since 1999, when he played in the AT&T Canada Senior Open Championship. Monday qualifier Tom Ahern won that tournament in a playoff over World Golf Hall of Famer Hale Irwin.
Quigley was also here in 1994, shooting a 6-under-par 65 to win a pro-am event on the eve of the Quebec Open at Golf Dorval. Stephane Talbot won the tournament.
"I have a lot of fond memories of Montreal," Quigley said. "Lot of friends up there, too."
Quigley, his daughter Nicole, wife Angie and Devon's mother Charlotte (Quigley's ex-wife) have maintained a bedside vigil at the hospital since Devon's accident. The family set up a website (caringbridge.org/visit/DevonQuigley) to share news of Devon's progress.
Here's part of what Quigley wrote on the evening of April 22:
"Hi Devo clan, hope your weekend was good! Devo's was pretty good. He got some time in the sun, and got some nice colour. He actually did a few more new moves today, and Nicole sent videos to everyone! She was massaging his foot, while his leg was straight, and Devo pulled his whole leg back toward his chest! It was really a sight to behold!
"Then she did the same thing with his other foot, and Devo responded by pulling that leg back! Wow! It was fun to watch. God continues to throw us a bone every day, and it certainly pumps us up! He gives us a little something every day, and it proves to us that his brain is connecting with some of his motor skills. Even though we are not doctors, we all know he's getting better!
"Sure glad God is on this caper! He may incur a two shot penalty for slow play, but we don't mind, take all the time you need, God! Just a little golf humour, hope no one is offended! God's ways are amazing, I'm so glad I get to witness His love every day!"
At the time of the interview, the Quigley family was still awaiting word of whether Devon would be transferred to another hospital. He could be moved to the Shephard Centre, a catastrophic care hospital in Atlanta, or the Spaulding Rehabilitation Hospital in Cambridge, Mass.
St. Mary's Medical Centre is less than 10 minutes from Quigley's West Palm Beach home, where Devon lived with his dad.
"It's tough, but we're doing OK," Quigley said of the family's constant vigil. "We have a great support system. His mother is with him probably 18 hours a day. His sister's with him all the time."
The family agreed that Quigley should continue to play on the tour as much as possible, believing it was what Devon would have wanted.
Like his father, Devon also played college golf at the University of Rhode Island, graduating in 2007 after having been on teams that won New England and ECAC Championships and competed in the NCAA Regionals. In March, Devon was named a graduate captain of the 2011-12 Rams golf team, and players and coaches are wearing a "Devo" pin on their caps.
Father and son were regulars at their home course at Bear Lakes Country Club in West Palm Beach, and were scheduled to play together the morning after the near fatal car accident.
"I play every day in the morning," Quigley said. "I'm through by noon and then I come up to the hospital and just hang around and talk to him. I spend a few hours before dinner every night and stay until 10 and 11 at night.
"We talk to him like he knows everything we're saying, which would be really good if he does.
"I watched the Masters with him and talked to him all day about the final round on Sunday, telling him about all the shots and everything. I asked him the next day if he remembered that Bubba Watson won and he raised his eyes, which means yes."
Quigley has won 11 times on the Champions Tour since 1997 and has amassed $14,819,592 in earnings – fourth on the tour's career money list.
He qualified on the Monday for four of his first eight starts before winning the Northville Long Island Classic in a three-hole playoff over Jay Sigel.
It was an impressive start for the native of Lynnfield Centre, Mass., who turned pro in 1971.
Quigley had a relatively nondescript career on the PGA Tour after winning numerous tournaments at the club professional level, including the New England PGA Championship five times and the Rhode Island Open six times. His best season on the PGA Tour was in 1980, when he won $31,509.
Quigley won twice in 1998, his first of three two-win seasons, and won at least once in four consecutive years from 2001 to 2004.
In 2005, with victories at the MasterCard Championship and Bayer Advantage Championship, Quigley capped his best season by winning player-of-the-year honours and, at age 58, became the oldest player to take the Arnold Palmer Award as the tour's leading money winner. He earned a career-high $2,170,258 and was the only player to eclipse the $2-million mark that season.
"I continue to play because I love it," he said. "I don't hit balls. Until Devon got hurt, I'd play 36 or 45 holes every day. Now it's only 18.
"Devon would play with me all day long, and once he gets better we'll be back out there together.
"I still have an unbelievable passion to play golf," Quigley added. "Some days are a mystery and I should hate it, but other days I can't wait to get out there. That's the beauty of the game."


text:
This coming week is the Frankfurt Bookfair. It's the biggest book fair in the world! So imagine my excitement when I found out my book is going!
My humans often tell me I'm special but we all know that they say and do silly things, so I've never taken them too seriously. However, I'm very honoured to find out that my publishers also think I'm special and my book is going to be a special title!
This means they've made a dummy, it's a mockup of how my book will look, with the cover and some spreads. It means people will have an idea how the finished book will look…
If you look closely you can see me swimming and completing my cat overboard drill!
I think it looks pawesome! What do you think?
Bisous Bailey


text:
- Product Features
This product has been discontinued and has limited stock available. Please contact our sales team for current stock levels.Create impressive eye catching light installations with 120V Daylight White LED Super Flat Rope. 120 Volt LED Super Flat Rope is rugged, like LED rope, but is more compact and brighter. We are proud to announce that 120V LED Super Flat Rope is also UL Listed and runs off of 120VAC so it is convenient for plug and play applications.
Each end is double injection molded with twist locking connectors. All lengths and colors of 120V Neon are linkable from end to end, with a maximum run length of 40 meters. No need to cut the product! Cutting will void the product warranty and UL listing.
120 VAC LED Super Flat Rope is capable of producing lighting effects that were never possible with traditional rope light. Easy to control using our Lutron ELV Dimmers, DVELV-303P or SELV-303P-WH. This is a fantastic product for commercial, studio, architectural, and decorative purposes.
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- Maximum run length is 40 meters.
- Daisy chain up to 40 meters worth using one power cord (120V-LN-SFR-PC). Power cords are sold separately. (see Accessories tab).
- Specifications:
- Daylight White
- 120 volts AC.
- 6 watts (1.8 watts/foot = 6 watts/meter.)
- Length: 3.3 ft = 1 m.
- Width: 0.61 in = 15.5 mm.
- Height: 0.24 in = 6 mm.
- LED spacing: 0.47 in = 11.9 mm.
- UL Listed, RoHS, IP65.
- Cutting increment: 19.69 in = 500 mm. Not UL Listed if cut.
- 120V Neon/SFR products operate on standard 60Hz power. Dimming at this frequency may cause flicker when recorded on certain cameras.
- Minimum bending diameter is 30 mm - bending the LED Super Flat Rope more than this will cause damage.
- Do not hang or suspend LED Super Flat Rope without proper mounts or supports.
- 50,000 hours expected lifetime.
- Warranty: 2 years limited.
- No Ultra-Violet emissions, so it won't harm your art or documents.
- We use high quality LEDs for maximum color vibrancy, longevity and a well-balanced 'all-on' white. We pay more for our LED components, and you'll benefit from the difference. This is not a commodity product. Quality matters, and the materials, controls, connections and tech support we provide will make your project successful.
- Cut LED Super Flat Rope is not returnable.
- We provide detailed installation instructions, design criteria and technical support upon request.
- Applications
- Specifications
Ambient Temperature -20°C to 45°C Average Lifetime 50,000 hours Beam Angle 150° Brightness 476.8 lumens/meter Efficacy 79.5 lumens/watt Brightness 2 1533 mW/meter Efficacy 2 255.5 mWatt/watt CCT 6,000K CRI 80+ Height (English) 0.24 in Height (Metric) 6 mm IP Rating IP65 Input Current 50 mA Input Voltage 120 AC LED Density 60 LEDs/meter LED Node Size 2835 LED Spacing (English) 0.66 in LED Spacing (Metric) 16.7 mm Length (English) 3.28 ft Length (Metric) 1 m Linkable up to 40 reels Manufacturer EnvironmentalLights Min. Cutting Increment (English) 19.69 in Min. Cutting Increment (Metric) 500 mm Power (Watts) 6 Power (Watts/ft) 1.80 Power (Watts/m) 6.00 Rating UL Listed, RoHS, IP65 Warranty 2 years limited Width (English) 0.61 in Width (Metric) 15.5 mm
- Documentation
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text:
sea, salt, smile, sand, sun, shore, steel, stone.
hello esa.
sea, salt, smile, sand, sun, shore, steel, stone.
hello esa.
i thought i would run around with the camera during the graduation party, but nope. it was time for socialising!
but i captured some pictures before the sixty hungry guests arrived.
bon appétit!
by the way, i have never seen this beautiful butter XD
most of the decorations eventually ended up in my tummy - wow new flavours!
a great party is nothing without great people.
but there isn't even an incentive for a party without some pearls behind the scenes.
ina and cami: thanks for the contribution and the company!
the "surprise" i mentioned before was a speech i held to this (not so small anymore) brother.
thanks to all guests and also a big thanks to the weather gods.
in my speech in mentioned that he'll manage whatever he chooses to do. he's practical and calm as a filbunke
= a pot of sour yoghurt ;) ?
yesterday he got good news: he's starting the flight mechanics programme in the army in july!
... follows sun? i hope.
my mood follows the barometer.
too much thinking - too little done during these past monotone monocrome melancholic days of rain.
but enough of them!
first i want to share janccu's new analogue photo project.
i happened to be day 3 ;)
actually a suitable expression for this week.
men mungiporna upp!
today i'm full of pride and expectations.
my little brother graduates from mattliden's gymnasium and it's party time.
but i'm a bit nervous and i'll let you know why later.
alexander's achievement brings to mind my own graduation 2,5 years ago.
although my IB graduation is a fine memory i'm sincerely lucky that things have changed - a lot :)
loving life, family and friends.
allu is already a top mark bachelor of law already and is next semester returning to england for masters level.
we celebrated her with good food, great discussions and a passionate music performance by stella & paul.
... but what else than a fantastic show could you expect from a satin circus pop star + a fresh bachelor of theatre art
also my saab is back from scotland over summer and the two of us "prepartied" in the summer heat at the world village festival in kajsaniemi.
allu's symphathetic dad entertained us with his guitar and groovy singing voice.
stella got to try cognac from this 3-in-1 glass ;)
me, cami, paul & stella and andu focused on the strawberries. the more allu brought in, the more we emptied ;)
but also the cake à la allu was a success: loads of fillings between 4 mud cake layers!
and then i biked home to be fit for fight for work at the eu elections the whole sunday :D
ina and i laughed at this petit gourmand.
eating ice cream is emotional and engages the whole body!
by the way, i am selling clothes at syrrans garderob!
sun, sea, sausage, SUP, smiles = summer
ina got paid for the second cup of coffee!
the café actually has its own potato land in this flower box <3
and people buy or bring their own sausage to grill.
looks good. i'm happy to have found delicious vegan versions. they even look the same, so i can belong to the "sausage gang" this summer!
another thing which i definitely want to try this season is SUPping! they even have some for rent at the café.
i'm currently more active on instagram, so please follow me there as well :)
i seem to celebrate summer vacation - also from blogging.
but here finally comes the grande finale of the congress in cologne.
i love spending time in the summer heat outside, but (new) friends actually will trigger our hearts to produce some heat by themselves.
the last evening we hired a whole bar to organise an auction.
all delegations brought a bucket of specialities from the homecountry to raise money for fejs.
i brought mignon eggs, reindeer ryebread crisps and marimekko napkins ...
finland + turkey gave the highest bid on the german package, and here you see what i got to carry home ;)
fejs was all about networking and to create social webs.
but i also happened to unravel some personal meshes.
mari found this spooky tree with spider? webs.
i took a walk alone and reflected about everything under the sky.
... but of course it was not deep intellectual talking or thinking all the time.
while mari visited a french style bakery i captured this huge bread cow ;)
the best thing about fejs were the friends i made there.
we had a great finnish delegation on 5 girls - but none of us knew each other from before!
i had a spent a lot of time with empathetic mari. here we are badasses skipping a lecture to eat strawberries in the sun ;)
girls going out.
travelling is nothing without people watching.
cool "colognials"
my blog still breathes german air, but i already celebrate summer holiday in esbo. now it's time for my brother's birthday party!
today i was a guest in the radio talk show pia & peter on the channel vega. i enjoyed the relaxed and inviting atmosphere in the studio.
you can listen to me afterwards here (but only in finland i think). i'm there during the whole show, but i'm introduced at 18:10.
reflecting over my experiences in cologne.
could this be the trip in a nutshell: the dome, spooky skulls and a journalist?
well, maybe not the skulls (although i adore them)
... and the nut needs to be a bit bigger to fit all the other 99 journalists as well ;)
so, the fejs congress i cologne is hard to summarize with a few subjectives, but i'll try with 3 adjectives instead:
inspiring, social and exhausting.
we had a free afternoon to do some "home work". when it was done i took some time to stroll around sunny cologne.
i found a mix&match müsli shop!
a pity that they ran out of all the glutenfree ones. it would have been the perfect gift for mom.
the 1st day at the FEJS congress for young journalists.
panel debates, new media, multiculturality, copters, interaction, innovation
... and tons of questions, but what else could you expect from 100 journalists ;)
the phone camera is just perfect for capturing clichés.
the sunny day made me long for frozen yoghurt, but they were out of lactose free in the two bars i ran in to.
the journalism congress in cologne started out promisingly. i spotted two dalmatians on the street ... and later in a shop!
although the germans don't actually celebrate the 30th of april as we do, the whole city was full of life out on the streets all evening.
but what i disliked was all the tobacco. they should take example of thailand where national campaigns have been successful. the only people there i saw smoking where farangs, foreigners.
the bars looked however cosy and chill unlike many of our drunken places ;)
candlelight <3
i appreciate finnish safety, but forbidding candles (in many situations) is too much.
where is the ambiance in a sterile and perfectly safe place?
bringing arts and culture to hospitals, schools and backyards adds life quality.
sipping some vitamins with my new friend ugi. then good old janccu joined from school.
plants everywhere, so i asked ugi who takes care of them?
- everyone.
i wish this system would work in helsinki as well.
here's a neighbour in action!
i dragged my guide ugi to the photography playground in berlin.
fantastic light sculptures and optical illusions.
you didn't know that i also freelance as spiderwoman?
behind the epilectic curtains.
i see the light.
and a chameleon granny who fits into all situations.
climbing on a bike messenger.
around his corner i saw this cosy italian restaurant.
my host janccu outside my guide ugi's home in hip kreuzberg.
i spotted skeletons in the windows. my fetisch.
love the architectural variety in berlin.
jungle homes and roof chilling.
chez janccu.
germanwings to berlin.
bus in berlin.
train to cologne.
taking it easy right now, i need it.
tried yin yoga with my favourite "monkey" tiina mankki.
gathering sun energy and maritime air at fölisön.
tomorrow i'm flying to cologne to represent our uni at a seminaar for young european journalists.
but first i'll celebrate wappen in berlin with janccu :D
i wouldn't mind having my entire spring wardrobe from cos.
here are some window mannequins showing the sleek fashion of this season.
next door there is another source of inspiration.
shopping gifts made me hungry.
i turned tölö into asia with spicy quorn-buckweat noodle wok
... and continued across the pacific ocean with a carribean sweet potato-banana stew.
attn! only the first portions ;)
i am not one of the 10 handpicked participants of the photojournalism programme in aarhus.
of course i feel sad since it was, and still is, my dream.
luckily mommy was there to cheer me up.
we celebrated the day of books and roses over lunch at our favourite café.
the apple does not fall far from the tree ...
we both arrived a bit shameful with ugly plastic bags as the only wrapping.
inside both bags were cooking books ;)
mine about raw cakes and her's about salads.
luckily there are bright spots among the grey feeling of disappointment.
as plåster på såren mom didn't ask whether i also want a sweet piece of cake - she just bought two to share <3
i will not give up on aarhus, i just have to sort out my future right now.
i have known
luna since kindergarden:
maria since school started:
and i just met lilian and we share a lot of interests.
she showed me the café sininen huvila as we walked around tölöviken. i must have been blind to have lived in tölö for 1,5 years without noticing what a great place for a break it is.
feeling rich since i have met so many friends this week that are worth their weight in gold.
sometimes time just ran away and i didn't bother to distort a good moment by looking through the lenses instead of my own eyes. social experiences cannot be reproduced even with the very best camera.
already in april :D
the bridge was full of fishermen and -women.
while i and cami walked around drumsö, some people sunbathed halfnaked.
by the way, i love my lumia's camera, so handy and fun!
i really hope this new area will give a facelift to helsinki. not that i don't like it now, but it's never good to stagnate.
we climb a hill of stones for better views.no psysical effort to climb this mountain, but it's never wrong to take a coffee break.
sometimes i believe in gods - the weather gods - who blowed the first heat wave towards helsinki just in time for easter and our photowalk.
i made new friends and met lauri and atte who inspired me to start blogging.only me and the dudes shot photos. but we walked on jätkäsaari, the dude island, after all.
hanging at my hoods.
what could be better than this?
too hot for jacket
prime time with godson
and lots of fun plans today!
it's not that bad to be left "alone" for easter? i thought i would get bored, but you know me ;)
this easter is different. i usually spend a lot of time with my family, but my parents are photographing foreign nature.
this means time to catch up with friends, such as this philosophizing ballet dancer.
it is easy to feel lonely during holidays when everyone else seem so occupied with family dinners and traditions.
i have no special decorations this year, but got my share of good old easter at my grand parents house the other day.
easter awakes some thoughts.
is someone keeping a safe eye on us from above?
or are we marionettes in a game called tellus?
driven like hamsters in a wheel?
like in henrik vibskovs human laundry service.
i belong to the church, love the christmas mood and i'm proud to have a godson to take out for a easter froyo brunch at yobot. but i wouldn't call me a christian. i want to have a wider view of the world.
ecological, local, experimential, fair, classic haute couture & design in turquoise - that's what i and anni like.to see danish design accelerated my danmark fever - can't wait to know if i will move there in august!
reflecting over falling flamingos.
i have been a bit tired despite this awesome weather and a not-too-full calendar.
last week ina and i baked a veggie taco pie in my terrible oven ;). but it turned out yummy.
yesterday i met up with my best art exhibition company: anni. we both fell in love with this anorak of finnish design.
after that i biked to sörnäs to see a play that focuses on portraying humanity through movements and behaviour. among the actors are two uni friends.
still two shows left on saturday, but be quick if you want the cheap tickets!
and it rained. so my eyes almost felt like raining some tears too.
the sun suddenly came to save my calm weekend in gäddvik.
btw, here you can read my blogging story i swedish (scroll to page 7).
i was against instagram ever since it started. to post retro images with light leak effects felt like cheezy cheating. i did the real thing with my plastic film camera diana.
but i haven't played with her since i became petitemu. developing films is expensive nowadays and i got thrilled by the speed and easiness of shooting only digital.
my new yellow aquaintance constantly blipped about an awesome app called instagram.
fine then. jepardqvist is in the house!
consious cycling.
better than b&j.
am i throwing out an old toy for the new mainstream one?
i hope not.
diana will always be my number 1 and is great company at summer parties for example.
while waiting for my 9-year old kiddoes to arrive to my lecture about refugees in uganda, i felt like becoming small again. luckily i have a new toy to play with.
i have had a fetisch for neon these past grey months, and i don't fell like stopping.
today i will host a studio debate in tv, so jump into the coach and watch me here.
now i almost regret that i cut my hair. otherwise i would have got a nice hairdo while sitting in a 50s salon.
i cued a while for some makeup, but then realised i was spending the evening all alone.
i would have waited for sure if mr brylcreem would have been waiting for me ;)
finnish skiing fashion certainly has changed.
but the round pair of sunglasses could be mine. my brother calls my ossy ;)
guess who these lovely legs belongs to?
who else than the winner of the costume competition.
these 1st row-ladies were pretty from all perspectives:
helsinki couture from the 50s.
i thought i took the tram, but it must have been the time machine.
everyone is longing for cool cuisine: hotdogs!
which is the odd one out?
answer: the smart phone.
i visited villa hagasund again. this time with a real touch of the fifties.
there was a costume competition. this handsome couple was crowned as most stylish.
even i made a small effort and came with dramatic eyes, an a-shaped dress, jewellery and half boots - just for fun.
time to wash the car - in a t-shirt when it's 4 degrees.
this was when i decided to put my wool scarf into the bag and celebrate spring.
say hello to mr motor.
i guess he's jätkä enough for jätkäsaari.
busholmen is probably the oddest part of helsinki. i travelled there to buy a new phone and to take a look at the helsinki photo biennale in galleria huuto.
i have seen a sneakpeak of the biennale also in the photomuseum, but the rest parts are still on my todo-list. this year's theme is a fascinating one: ecological fallacy.
today i also heard a lecture about refugees by a swedish digging journalist. interesting, informatic and important.
there i was served a delicious bowl of fish soup. why haven't i eaten it for ages?
another professional idol of mine is jeanette björkqvist. she tried out as a playwright, which i wrote an article about in ny tid a while ago.
this week i got to see the performance kvinna till salu about sex trafficking.
like a horror movie - but true.
i saw rasvaletti at villa hagasund with mom and bought some pastry from a cosy glutenfree cafe in the garden.
laura and i tried out some cool ideas for the restaurant day.
so the sporty blondies baked vegan glutenfree protein blondies.
and of course we had to eat the treat!
even moomin knows that goji berries and blondies energises a swimming tour ;)
my old girl's gang is like sisters for me. we had a lazy sunday brunch in our childhood hoods.
cami i have known all my life, allu since school started, jonna from 3rd grade and ina joined at 7th grade.
the rest of the best life elsewhere in finland and abroad.
i felt a bit weak, so drank a super C in my favourite colour.
expectations. jonna (far left) and allu (bottom right) flew the same day to budapest for an interrail across europe.
i wish you all the best!
then i took a look at the emma museum's temporary exhibitions.
i <3 the combo of fresh organic food, cool company, concrete architecture and modern art.
i already have denmark fever, even before i know if i have the chance to move there.
i sent in an application to study photojournalism in aarhus.
please keep your fingers crossed while checking out my portfolio.
in aarhus they have a perfect exhange programme, but ...
the big but is the fierce competition. they actually require at least one year's studies in photojournalism - impossible here in finland.
here are some oldies from a family trip to copenhagen in december 2011:
i got an unique opportunity to attend the photography expedition by pata degerman.
i knew that he was a mountain climber, an adventurer and a family father - but not that he's a natural comedian, a painter, a nature expert, a businessman and a fantastic photographer.
when a polar bear comes too close he rather shoots with a camera than with a gun.
a walk from uni/work together with this lady last thursday.
we heard birds singing in the evening sun and these pics are from the place named the birds song.
i will spend monday evening in this old people's home. i'm doing a publication for the foundation.
laura organised the best housewarming party i have been to:
1) the food: 1000 sorts and flavours.
we decided to take part in the upcoming restaurant day on the 17th of May, so stay tuned!
raspberry raw cake (my favourite) and lime cheese meringue cake (most people's favourite) = both top class.
2) the big brand-new appartement with fresh scandinavian interior design. i stayed there all night.
3)great guests that i didn't know from before.
"just" some fireworks over the neighbourhood. the reason is unkown.
sawatdeekaa thailand!
but now it's time to move on.
... could as well be photos of me ;)
these two tall mammals - luna and the giraffe - got along well.
from "inside" a jungle bathroom ;)
sometimes i have a bottomless stomach, but it's nothing compared to the apetite of an elephant.
baby shower - literally.
an afraid aunt?
show business is exhausting ...
colourful company.
there are 6 albino tigers in the world - i have seen one of them.
i'm sceptic to keeping animals trapped in cages and turing their life into a circus, but here at the open zoo they seemed quite alright.
i admit it was cool to see them from such a short distance.
why see red when you can see pink?
i have been back in snowy sunny stressy finland for 2,5 weeks already, but this is my way to keep on relaxing in bangsaen.
chilling in the museum shop.
we didn't dare to sneak into the actual university corridors because of all the guards.
but i wouldn't mind to be one of the over 46 thousand students at the university.
do i have time to apply for an exchange programme here next autumn? just in case i'm not accepted to aarhus.
just passing by a health exhibition.
we didn't understand any of the information, but i hope this wasn't a mirror that shows ideal health - i look more like being tortured in a rack ;)
there are not many museums in bangsaen, but the university is specialised in marine biology.
looking at aquariums is fun whatever age you are.
the stingrays are my favourites!
luna and the mola mola.
la mode de bangsaen.
luna was the bravest. she just pointed and ordered something, while i involved a whole crew of street chefs to order meatless and my pet.
luna's lunch turned out to be delicious and very spicy, mine more safe, less cheap but tasty.
to cool down our tongues we ordered thai style icecream served from a coconut.
once again luna dared to take all toppings included.
the twenty eleventh bubble tea café ;)
we bought some mouthwatering snacks from this lovely man and his wife ...
but, before we were out of the car on the monkey hill most of the goodies were gone! argh!
that's why we didn't buy them any bananas - the theft was enough!
they were humane, but in a grotseque and ... animalistic way.
wanna eat my fleas?
the weekend street market at bangsaen beach.
bangsaen lacks foreign tourists, but bangkokers drive down here to relax, eat seafood and enjoy the ocean during the weekends.
music - thai people can't stand silence.
i tried a comfy and nice dress, but it was too romantic for me.
this made me smile:
walking, eating, shopping, eating, talking, eating ...
choose between: sushi, colourful bananas, cakes, fruit candy, spicy noodle wok ...
on my plate: a vegetarian tofu "omelette" with vegetables
and sweet white ice tea with chrysanthemum.
hungry dancers ...
and brave farangs!
we tried 1 fried grasshopper each. it was tickling my throat but luna liked it.
friday is a perfect day for a makeover.
step 1. here is a hint:
no, not a tattoo!
but it was exiting enough to make a new piercing without common language or methods.
i have no idea how he made it, but i guess it was with a needle.
i was afraid to end up with a huge hole in the wrong place, but i'm completely satisfied.
next step was at the hairdresser's. i have never let someone random cut my hair before.
she cut less than i wanted, but better so. it was cheap and friendly.
less than 8 euros for me and my aunt.
yeah - here is mr piercing again!
luckily i got phompens son and half of his class to assist me.
bangsaen is a coastal city with world-famous oyster farms.
and a daily market for fish and seafood.
fresh fish for breakfast and dried into the suitcase.
these girls know that eating fish leads to a healty happy life.
and now i don't mean finnish fish fingers ;)
our nearest temple is a really neat and nice one ;).
the whole area is full of statues that show what happens when you sin.
luna has tried alcohol - too bad for her!
luckily she gets to feed the turtles before her destiny.
this cute couple was tall as a block house.
the only right way is to obey the buddhist rules.
and donate money!
another nearby market.
i had to try an octopus sushi.
i bought a new towel for the gym: with stitch!
belly bag with skeletons, cheap sunglasses and green lipstick.
we celebrated lisa's last day in thailand with a cooking night in her family's grand penthouse.
i was more than happy to see that a favourite - fresh spring rolls - were on the menu!
chefs in action a cute little thief.
lovely pim planned pure vegetarian alternatives for me.
the fresh tofu with pickled carrot was delicious!
we cooked and ate thai style - anywhere and all the time!
but i managed to roll more than i could eat
-> doggy bag ;)
too much veggies for pai? he had his own meal.
- i take a phramed luna, thank you!
how much? can i get any discount?
anything can be bought at a thai market.
this is the main street from the beach to the mall.
feels like home, i miss it already!
see no evil
say no evil
hear no evil ...
and do no evil?
at wat arun in bangkok.
the world's highest open air bar. we didn't visit - unfortunately ;)
a poor phone picture through the bus window, but it should show an intense forest fire on our way home.
temple wat arun on the shore of the bangkok river.
i needed to hire some clothing to cover my legs whereas lisa and luna found other ways.
muscular and tall lisa looked rather riddiculous in my petite shirt ;)
the stairs to the temple felt like straight up towards heaven!
maybe noodle time after climbing the stairs?also the monks use collective traffic.
directly after koh samet we headed for a one-day-trip to bangkok.
this was one day after the bombing. therefore we only hopped on and off the river boat at safe locations.
pim, luna and lisa. we really had a great time together <3
laugh as much as you breath and love as much as you live.
china town in bangkok was crazily cheap and fullpacked of goods.
we also ate lunch for 70 cents/person.
pim recommended me a new look and my company seem to have a fetisch with hats ;)
we drank pure pomegranate juice and lisa bought a dessert with coconut and colorful "worms".
more fooding.
our koh samet rooster + the neighbours karaoke night = not much sleep.
luckily we're on vacation ;)
rabbits seem to have good taste: watermelon!
snorkling tour from koh samet. only 13 euros for a one-day-tour including lunch!
i caught a crab and got niped!
i also dared to lift a sea urchin but luckliy none of us stepped on one.
our hopp-iland-kalle
a light start.
continued even brighter.
i'm on fire, but luckily a wave came to cool the fire limbo down.
sand in the pants, water on the toes and delicacies in the tummies.
when you wish upon a ...a dessert favourite: mango sticky rice with cocnut milk.
3 (ugly) blondes training for the snorkling tour.
photoshoot on a catamaran.
the model smiled at me - the stupid farang who tried to capture him ;).
we also met some less pleasant creatures.
my aunt got a bad scratch by a cat who probably carried rabies.
and mr snake was visiting the neighbours when i passed by.
i also walked by a yoga class in the nearby studio.
did neither dare to try thai yoga nor these angry birds sushis.
here still pale as porcelain, but not anymore.
ironically i caught a flu in thailand.
now curing it with sweet and lovely thai tea soya latte.
our café chics were adorable and we became regulars ;)
the shadow of luna.
beach crayfish. koh samet gym.
our hut was meant for me
with singapore key.
we had a cool blowfish lamp!
the cycle of life of a thai frog - a tragedy.


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A spectacular physical features portrait of the United States. This stunning map, published in October of 1996, compiled information gathered from multiple sources to create this look at the physical forces that have shaped our landscape. A companion to the Federal Lands map published the same month, this is a must have for anyone interested in geology or Earth Science.
Coverage Area: United States


text:
Rail Runner Station hit by vandals
Culprits not found
Criminals sprayed an entire wall at Rail Runner Station with graffiti and recorded themselves vandalizing the property on their smartphones.
But what they didn't know was a security camera caught them in the act as well.
Officials with Rail Runner station are asking the public to watch the video attached to this story very closely.
There are three males, who appear to be teens, vandalizing the property.
A young man is caught recording the spray-paint job on his smartphone.
"They went about it in a very methodical fashion, covering the whole wall," said spokeswoman Augusta Meyers.
Then the camera zooms out and you can see three so-called graffiti artists. One of them is holding a large spray tank.
"They has some kind of apparatus that allowed them to get up pretty high," said Meyers.
On Monday morning, Bernalilo County's graffiti removal team doused gallons of solvent to eat up the paint, followed by water pressure to wash stains off.
Rail Runner said the cleanup job could cost up to $1,000 which is why they're hoping the public can help identify the culprits and force them to pay up.
"We are going to find out who these people are and they will probably face some serious charges," said Meyers.
Officials said it appears the graffiti on the wall is gang-related.
If you think you recognize the males in the video attached to this story, you are asked to call Crimestoppers at 505-843-STOP.


text:
There are just 2 days to go for the fifth edition of IPL tournament to begin and the excitement is already building up. One of crickets' most talked about tournament, IPL 5 2012 will start from April 4, 2012. This year's IPL 5 Opening ceremony will have performances from Katy Perry and Prabhudeva.
IPL 5 is one of the biggest cricketing tournaments in India at have matches at convenient hours. However, the online broadcasting of IPL 5 gives users an opportunity to catch up on the live action of the cricket matches while on the go.
This years' official partner for broadcasting the match online is Times Group's Indiatimes. Viewers will be able to catch up on all the live action for IPL 5 2012 on the Indiatimes IPL website at http://ipl.indiatimes.com. Users can also catch up on the latest IPL 5 news and scores on the official website at http://www.iplt20.com.
Indiatimes got the telecast rights for IPL after the initial online broadcasting for IPL 3 had gone to YouTube. However, the broadcasting of IPL 4 in 2011 was later jointly streamed by both Indiatimes and YouTube on Indiatimes official YouTube channel. Users can also watch the IPL broadcast on Indiatimes official channel on YouTube at http://www.youtube.com/indiatimes. Additionally, you can also find more links to watch IPL 5 2012 Online.


text:
Sticking to the time-honored principles of teamwork and talking, Nipmuc Regional, 7-4-1, is on track for another date in the postseason.
"At Nipmuc, we're all about teamwork and before each game we meet and make sure we emphasize teamwork and its meaning," senior captain Jessie Alibozek said.
Teamwork has worked in the past for Nipmuc, which won 12 games last season and qualified for the Central Mass. Tournament. This year's team, which has a good mix of seniors, juniors and sophomores, started strong, winning its first four games.
"This is one of the most talented teams that I've played on," senior captain Kaitlin Massey said. "Besides the defense, we communicate really well, and that's always a key. But we do have to work on some little details, like getting our sticks down and making sure we're covering everyone. Our coach (Sarah McIntyre) is the best. She'll keep us playing together. She really knows the game."
Massey, a defender, is a familiar name in Nipmuc field hockey circles. Kaitlin is the third Massey to serve as captain. Older sisters Erin and Michelle both went on to play college field hockey at Salve Regina after standout high school careers.
Alibozek, a midfielder, said that Nipmuc has been boosted by its commitment to defense, which goes beyond the physical.
"We have a great defense and I think what works really well for us is that we always cover for each other," Alibozek said. "If one person gets beat, there's always someone else there to cover for her. We play a lot of good teams, so we have to play the good defense and be there for each other."
Senior captain Meaghan Roche, a goalie, said that Nipmuc has a nice mix of veteran players and newcomers who are getting better. The improving-each-game process has been helped by the fact that many girls have basically stepped in, and up.
"I like the way our team is playing — things are going really well," Roche said. "And it should get better when we play the teams we already played and pick up on the mistakes that we've already made. There are still a lot of tough games on the schedule."
The Warriors' offense is led by junior forward Danielle Schiloski and junior midfielder Juliana Miccile, both top ten scorers in the Dual Valley Conference.
Other contributors: seniors Sara Dodd, Maddie LaPlante-Dube and Emily Davidshofer, juniors Erin Kelly, Maddie Killeen, Aislyne Calianos, Taylor Russell and Karlie Jango, and sophomores Maddie Davidshofer, Anna Young and Lauren Guertin.
The Warriors battled Wachusett Regional to a 1-1 tie yesterday in a crisply played game dominated by defense.
"A tie is better than a loss, but a win would be a lot better," McIntyre said. "This was a game that we could have won. There are things we need to work on. That's what practice is for. Hopefully, we can fix those issues in practice this week and get some more wins."
Bromfield coach Sue Silver is hoping that a hot second half will propel her graduation-depleted contingent into the Central Mass. Tournament, annually a sure bet for the hard-working Trojans.
Bromfield, which has moved from Mid-Wach D to the Wachusett A League, begins this week with a 4-5-1 record. That's not bad considering the array of talented teams in the league, headlined by pace-setter Oakmont Regional, Littleton and Narragansett Regional.
"We have a tough schedule — every game has been a struggle," Silver said. "We lost quite a few players and have had trouble scoring."
Bromfield, which won its league last year and qualified for the postseason, had a strong 3-2 early-season win over Sutton, but has been beaten by Narragansett (twice), Lunenburg and Oakmont.
The Trojans are led by senior captains Haley Newsham, Laura Jarvis and Anna Lee.
Newsham has moved from center-midfield to sweeper, bolstering the defense. She has excellent stickwork, moves the ball and sees the field well. Jarvis plays right midfield and also has good stickwork. Jarvis uses her skills to launch a lot of shots and jumpstart the offense. The hard-working Lee plays left back and is consistent. Lee also helps out with younger players, coaching the middle school team in the winter.
Junior center-forward Gabby Doben is the team's top scorer, totaling six goals and two assists. She is quick, skilled and handles the ball well, particularly on breakaways. Sophomore Colby Newsham, Haley's sister, has moved into her sister's former position at center-midfield. Colby has adapted well to her new role, switching from defense to offense, and sports good stickwork.


text:
An autopsy conducted on Sunday revealed that Abed Fattah al-Sharif, the 21-year-old Palestinian shot dead last week after a stabbing attack in Hebron, died from the head shot caught on camera.
Even though Israeli medical sources did not confirm the findings, a Palestinian pathologist present at the autopsy said the direct cause of al-Sharif's death was the bullet fired to his head at close range by an Israeli soldier.
Doctor Ryan al-Ali, director of pathology at the forensic institute at Najah University in Nablus, told Palestinian media that he agreed with the Israeli team that al-Sharif was wounded by several bullets in his arm, shoulder, lower body and chest, but none of the wounds were lethal.
"The heart and main arteries were complete and therefore it is clear that the bullet to the head caused death because his entire skull was smashed," al-Ali told the Israeli newspaper Haaretz.
The Israeli Supreme Court denied appeals to allow a Palestinian doctor to partake in the autopsy itself. However, the court granted permission for al-Ali to attend the autopsy and takes notes.
A video released by the Israeli human rights organisation B'Tselem captured the moment the Israeli soldier shot al-Sharif in the head, after he had already been injured and left lying on the ground.
The autopsy was conducted to determine if al-Sharif was killed by the first gunshots that incapacitated him or the bullet fired at close range to his head. The results dispel the claims that al-Sharif was already dead when he was shot by the Israeli soldier.
The soldier caught on camera claimed he acted in self defense, and is currently facing charges of manslaughter.
"We are concerned this killing may not be a lone incident," said Rupert Colville, UN's High Commissioner for Human Rights in a recent statement. "All incidents where security forces have caused death or injury should be fully investigated, and those responsible brought to account," he added.
The wave of unrest that started last October killed nearly 30 Israelis and at least 200 Palestinians, predominantly in lone-wolf stabbing attacks carried out by young Palestinians against soldiers and Israeli civilians.
Most Palestinian assailants have been shot down by Israeli forces, which have been criticized by human rights groups as "unlawful executions" and "unjustified use of lethal force."
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text:
III. Prevailing Wage Rates
What wage rates must be paid to supervisory employees (foremen, superintendents, etc.) employed on a covered project?
The wage rates for bona fide supervisory employees are not regulated under the Davis-Bacon and Related Acts because their duties are primarily administrative or executive in nature rather than those of laborers or mechanics. However, such employees who devote more than 20 percent of their time during a workweek to mechanic or laborer duties are laborers and mechanics for the time so spent, and must be paid at least the appropriate wage rates specified in the wage determination. Employees who are bona fide executive, administrative, or professional employees as defined under the Fair Labor Standards Act at 29 CFR Part 541 are not covered by the Davis-Bacon Act.


text:
From 2010.igem.org
iGEM Davidson – MWSU 2010: Measuring Gene Expression
The system we originally designed to address the knapsack problem had several vital components. The TetA and RFP genes, along with their corresponding gene products, served as our reporters to see if and how a cell solved the knapsack problem. However, we recorded several observations regarding gene expression as we progressed. Characterizing and attempting to understand these foundational problems became one of the new focuses of this team.
Results from past Davidson and Missouri Western research hinted at the presence of a transcriptional terminator within the TetA gene. Any gene located downstream of the 3' end of the TetA gene did not appear to be expressed. This gene codes for an effluent pump that physically removes the antibody tetracycline from the cell.
To test for the presence of this terminator, we created a construct with a fluorescent protein gene downstream of TetA. If TetA really does block transcription, we would expect to see no fluorescence despite induction of the promoter that precedes the construct (pLac+TetA+RFP). We also tested pLac+TetA+RFP to see if the gene products provided protection from tetracycline compared to a negative control..
The data suggest that the IPTG (0.5mM) inducer decreases cell density in all three cell types. In addition, the construct with TetA before RFP was only able to survive the presence of tetracycline (50ug/mL) when the inducer IPTG was present. These data imply that the inducer did increase gene expression even if overall cell density decreased. The construct fluoresced very little in comparison with the positive control (pLac+RBS+RFP) no matter what the conditions. The data support the hypothesis that there is a transcriptional terminator in the TetA gene.
We performed another battery of tests to compare the pLac+TetA+RFP construct to a construct with the pLac+RFP+TetA orientation. When compared to cells with RFP followed by TetA, the construct's negligible fluorescence was even more apparent. Interestingly, the cells with the flipped orientation grew poorly in the presence of tetracycline but had a high fluorescence per cell. One possible explanation for this phenomenon is that the fluorescence is divided by such a low number of cells that small variations are magnified. Alternatively, the presence of tetracycline could be selecting for cells with high expression levels of both gene products. These few cells are producing high levels of TetA effluent pumps in order to survive. Correspondingly, they are producing a lot of RFP.
MG 1655 E. coli cells have a genotype that closely resembles naturally occurring E. coli. We did a direct comparison of RFP gene expression in MG 1655 cells vs. JM 109. The data we collected is shown in the graph above.
We also wanted to see if the MG 1655 cells were more prone to induction by IPTG. The data for this experiment is shown in the graph above.
While working with two pLac-RBS-RFP clones, we noticed that one of the clones seemed to have a much higher copy number.In order to document the copy number we performed cell density tests and extracted the DNA and determined relative DNA per cell for each clone. The results from this experiment can be viewed above. We do not have an explanation for this variation in copy number.
In order to achieve a range of induction of RFP expression we attached a LacI repressor to the front of a pLac-RBS-RFP-RBS construct. We then proceeded to induce with combinations of IPTG and Ara. Placing the LacI repressor onto to gene affected the level of RFP expression. Results can be seen above.
During production of a commonly used expression casette, pLac-RBS-RFP, we noticed some colonies that produced a brighter red fluorescence than others. We picked a pink colony and a red colony and sequenced the inserts. We found that the DNA sequences were identical. We traced the difference in phenotype to the inserts by doing experiments that involved switching of the inserts between the two vectors. This led us to suspect that an epigenetic mechanism was involved in this process. The mechanism we proposed was DNA methylation. DNA methylation involves the addition of a methyl group to a nitrogenous base of the DNA. This modification of the DNA can be inherited through subsequent cell divisions and is known to regulate gene expression in a variety of organisms. Adenine or cytosine methylation (Dam or Dcm, respectively) is known to occur in many bacteria, including E. coli. Methylation could affect gene expression by making the DNA specific sequence harder to recognize by the polymerase; thus, decreasing gene expression. Methylation might also increase gene expression by having the opposite effect where the DNA binding site is more easily recognized by the polymerase, though this type is rare. We sought to determine if DNA methylation was actually playing a role in the gene expression of these E. coli cells. Our first experiment used three different restriction endonucleases that were sensitive to DNA methylation to see if any of the sites that are recognized by the enzyme are methylated. This experiment was not able to determine if any DNA in our gene was methylated. A future experiment that will help us in the determination of whether this gene is methylated is methylation-specific PCR (MSP).
Top Left = Red clone insert ligated to Red clone vector, Top Right = Red clone insert ligated to Pink clone vector, Bottom Left = Pink clone insert ligated to Red clone vector, Bottom Right = Pink clone insert ligated to Pink clone vector
In another effort to understand why the pLac-RBS-RFP construct was producing one set of red clones and one set of pink clones we designed an experiment to see whether the degree of red fluorescent protein was a function of the vector or the insert. In this experiment we performed a EcoRI/PstI digest on each clone and purified both the vector and insert for each. Then we put each of the inserts back into their original vectors as well as placing the inserts into the opposite vectors. The diagram illustrates the crosses. See pictures of the results of this experiment above. Fluorimeter data confirmed that the intensity of fluorescence in terms of Red or Pick followed the insert, not the vector.
As we were doing experiments with a pLac-RBS-RFP construct (part number I715039) we noticed some color variation between clones that were supposed to be identical. In an effort to trace the differences in fluorescence we spotted 2 ml of the two clones. These clones produced ring-like structures resembling a fried egg with a center, a middle, and an outer edge all of different colors. Sections from these colonies were picked, grown up overnight, and spotted again. Each of the new colonies showed similar results. These results suggest that variation in gene expression occurs in clonal populations of bacteria and that these variations are not genetically determined.


text:
I Have Not Seen, Yet I Believe
Solos to Sing, Submitted Music
1. They heard His voice; they saw His face,
The promised Savior come to earth in days long past,
They saw Him heal the sick and cause the lame to stand,
They watched as wind and waves were stilled at His command,
And though I did not see him calm the raging seas,
His hand has calmed my troubled heart, and I believe.
They saw Him scourged, And mocked to scorn,
They heard the angry crowd, They saw Him crowned with thorns,
They watched Him bend beneath our burden in the streets,
They saw the bitter nails that pierced His hands and feet,
And though I was not there to watch with them at Calvary,
My spirit weeps, I have not seen, Yet I believe!
2. They heard His voice, They saw His face,
The risen Jesus, crowed with vict'ry o'er the grave,
And though I did not see His triumph over death,
Though I did not see Him draw immortal breath,
I know He lived and died and lives again for me,
My faith is sure, I have not seen, yet I believe.
3. They heard His voice; they saw His face,
They heard His teaching of forgiveness, love and faith.
He blessed their little ones, He taught them how to pray,
He fed the multitudes who hungered by the way,
And though I did not taste the bread He bade them eat,
His word is manna to my soul, and I believe.
Music: Sally DeFord
Artist: Sally DeFord


text:
Walmart Protection Plans
Most items come with a limited manufacturer's warranty. The addition of a Walmart Protection Plan adds extra protection from the date of purchase. Walmart Protection Plans cover the total cost of repair, or replacement, for products, as well as covering delivery charges for the exchange.
Walmart Protection Plan options and pricing can be found on the product page, as well as in your cart. Go towww.walmart.com/protectionto see all the coverage offered for each product. You can view your Walmart Protection Plan after your purchase in the Walmart Protection Plan Hub.
Product warranty:
Warranty Information:All our products have 30 days warranty. 100% Satisfaction guaranteed. Contact us at firstname.lastname@example.com if you have any concerns and we will take care of you.
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A Single Venue to Connect Buyers and Suppliers across Multiple Industries.
Streamlined Buyer and Supplier Management
JAGGAER's robust Supplier Network connects buyers and suppliers across multiple industries and continents.
Our Network provides a single venue for customer-supplier collaboration, vendor discovery, supplier profile maintenance, and supplier catalog management. The Network is a platform that automates source-to-process, allowing buyers and suppliers to connect and realize the maximum benefit of their partnership.
JAGGAER also provides dedicated, HQ-based Supplier Integrations and Support teams to ensure buyers and suppliers are able to leverage the Supplier Network to drive value for their business.
Suppliers: Streamline Your Buyer Management Processes
The JAGGAER Supplier Network creates a single portal for suppliers to effortlessly manage buyers, track transaction details, create invoices, and more. It brings money saving transparency and efficiency into the relationship. Gone are the days of sorting for a single purchase order detail or payment in an email "wasteland."
The process of maintaining an online presence can easily become cumbersome. Many Suppliers offer hosted and punchout catalogs to numerous customers that require constant attention. Updating and maintaining profiles, and drafting, submitting and monitoring invoices, can all slow down the process. JAGGAER's Supplier Network tools make communications and transactions quick, transparent, and easy. The network allows for catalog management activities such as pricing and item detail updates, reporting, and connects suppliers to JAGGAER experts dedicated to integrations.
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The Supplier Network connects buyers to the right supplier for their business needs—whether the key factor is price, location, product availability, or something else, buyers can find the optimal supplier in an easy to navigate and trackable system.
Buyers don't have a problem finding suppliers—they are inundated with possibilities and requests. What they can benefit from is a more effective way to work with current suppliers and find new ones that will allow them to maximize margins and efficiency in their ordering activities. When there's a need to source a new product that current suppliers don't sell, or look for more budget-friendly alternatives, the Supplier Network helps easily guide buyers through 3,700,000+ supplier options to find the ones that are right for them.
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Supplier Network Members are Supported by the JAGGAER Team
Suppliers working with the JAGGAER Supplier Network can take advantage of our dedicated, HQ-based Supplier Integrations and Support teams that ensure suppliers are able to leverage the Network in order to drive value for their business.
- The Supplier Integrations team offers project management services for customers who decide to enable supplier catalog content in their shopping environment. The team members work collaboratively with both the customer and supplier to guide the enablement process to successful completion.
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- Access to 3,700,000+ suppliers—and counting
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Cracking the Spend Management Code: Integrated Supplier Management Software for Best-in-Class Results
Join Our Supplier Network
Whether you're a supplier looking to attract new buyers and streamline your relationships and processes, or a buyer aiming to navigate a sea of suppliers and find your way to cost-efficient arrangements, the JAGGAER Supplier Network can help your organization make the purchasing process—regardless of which side of it you are on—more efficient.


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Late last week, two bills came before the New York State Assembly that would have made it illegal to register cars purchased direct from manufacturers. That meant Tesla in particular, and the bills were more salvos their ongoing war with car dealers. But it looks like Elon Musk can breathe easy in New York for now.
Autoblog Green reports that the State Assembly ended their 2013 session late Friday without taking action on the two bills, making the issue effectively dead until they reconvene in January.
A rightfully relieved Musk tweeted this in response:
The kill Tesla bill in NY was stopped in the 11th hour due to public outcry. Am super grateful to everyone who helped.
— Elon Musk (@elonmusk) June 23, 2013
The bill was backed by the Greater New York Automobile Dealers Association, who have suggested that Tesla set up a dealership franchise system of their own just like Ford and Toyota and everyone else. Musk has previously said he doesn't think that would work because it didn't work for any other electric startup car company either.
Dealers have said that their system is better for customers because it allows for services like financing, trade-ins and maintenance. For these reasons and others, they have been pushing for anti-direct sales laws in a number of other states.
Personally, I'm more of the opinion that people should be allowed to buy cars however they want, and companies should be allowed to sell their products in just about any manner they choose. But car dealers have a lot of political clout (read: money) in nearly every state, so this is a fight where Tesla is definitely outgunned, even if they happen to be right.
Looks like Tesla has won a battle for now, but they have a long war ahead.
Photo credit Getty Images


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We all love to be in great shape. Our wardrobe has at least a few clothes that flatter our curves. That's where we should take some help from shapewear. It's a garment that is meant to flatter our curves and give us the confidence to wear figure hugging clothes. It's surprising that some women think that shapewear or a body shaper would help them lose weight. Sorry to break it to you, but that's a myth! However it will give you a flattering shape irrespective what every size you are in. A Zivame shapewear guide brings you facts that you must know!
Shapewear is meant to help you shape your body. It might visually make you look a tad slimmer. Some shapewear also comes with thermoslim and they are more effective than the normal shapewear. Shapewear have different levels of compression based on your need. The more you want to flatten or smoothen, use the higher levels. Shapewear helps you fit into the bodycon dress you have been thinking of throwing away, a tummy shaper smoothens your love handles instantly giving you a slimmer looking waistline, a saree shapewear is the perfect solution that replaces the traditional petticoat and the shapewear to smoothen the tummy and thigh curves.At Zivame, we know what you need and have all the right kind of shapewear online in India.
When you think of a Shapewear, do you know which one is perfect for you?
Shaping Camisole: Wear it under your tight top.
Waist Cincher: Perfect solution for your love handles.
Bodysuits: Pair it with your favourite dresses and jumpsuits.
Shaping Bottom Wear: Team it with your pencil skirts and thigh-hugging trousers.
Tummy tucker: It's the all time favourite of all women. Most of them love wearing it almost every day.
It means the capacity of a shapewear to smoothen or flatten the curves. The higher the level, the better the shapewear can control the bulges. Here are the four levels of compression for Zivame shapewear.
Level-1: Low compressionfor gentle smoothening. {8 to 12 hrs}
Level-2: Medium compression for tighter control. {6 to 8 hrs}
Level-3: High compression for a super smooth look. {4 to 6 hrs}
Level-4: Body-sculpting for a completely trimmed look. {2 to 3 hrs}
A saree shapewear is a replacement for your petticoat! A saree shapewear tends to shape your lower belly and thighs. The broad waistband is strong enough to hold any type of saree and especially the pleats. Be it a heavy saree like Kanchivaram ora light one like georgette or chiffon, our saree shapewears can carry the weight and make you look and feel gorgeous inside out.
At Zivame, we have all kinds of shapewear for your every need. You can buy shapewear online from our site or visit any of our stores all across India. Walk into our stores and our fit experts can get you the perfect shape wear. We also have Dermawear, Swee and Jockey shapewear for giving you more choices than you already have.


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Article content
Call it the perils of Pikachu.
A British man obsessed withPokemon Go was so focused on the, er game, that he fell onto electrified railway tracks.
tap here to see other videos from our team.
Pokemon Go player has leg amputated after falling onto electrified tracks Back to video
The result was that the man's right leg had to be amputated because of the severity of his injuries. He also suffered third and fourth-degree burns.
He had been looking at his phone when the accident occurred.
His legs, chest, arm and jaw were all affected and the man needed numerous operations to recover.
The incident has prompted a doctor to warn about the increasing amount of people he's seeing in emergency rooms who were injured — or killed — while looking at their phones.
The man was not identified.
The gamer was taken into the operating room where doctors had to cut off the burned skin and muscle.
He then returned to surgery two days later to have more flesh removed, along with his kneecap, which had started to rot.
The decision was made by doctors to amputate his right leg above the knee, leaving him with a "significant disability."
"Media coverage has reported on safety incidents involving Pokemon Go, including people becoming stranded, risky behaviour at the sites of known minefields and the unfortunate death of a teenager," said Dr. Kai Yuen Wong, who wrote about the case.
"Limb salvage was not possible in this patient. And therefore, in addition to his acute care, the disease burden will include the long-term costs associated with an above-knee amputation in a young man."
A study estimated Pokemon Go was responsible for 150,000 traffic accidents and 256 deaths in the first 148 days after the game's release.


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Xbox Kinect to ghost hunt ? Ghost adventures did !
Posted 07 September 2013 - 11:24 AM
It`s been awhile since I`ve been around, and I`m hoping everyone is doing well
I got a question for you, I was told in passing by my friends sister, that on an episode of one of my favorite shows ( Ghost adventures ), they used an Xbox Kinect to capture a spirit on screen.
I was curious if it`s possible, seeing as how the only time I`ve ever used one, was for video games, and it didn`t catch me all that well. I am assuming though, since I havn`t watched the episode yet, thatthey attached it to a laptop or computer and not the Xbox itself lol.
The episode in question through my googling the subject is titled * Cripple Creek*
I hope to hear your thoughts and ideas...on whether it would work or not... and if you had personal stories to share on the subject as well
Thanks for the read
PS. wasn`t sure if it should be posted here or not, but given it`s something that happened on the show... I thought I was in the right place to put it .
Posted 08 September 2013 - 04:53 PM
I am moving this to the Equipment board, as this is more about using an Xbox as equipment than it is about discussing a show. It'll get more attention in Equipment.
Posted 17 September 2013 - 07:51 AM
But for serious investigations one must use a device intended for the use it was designed for. It's like using a cell phone camera / recorder. It might work, sometimes, but the odds of getting a false positive far out weigh the odds of getting valid data. So if you want a camera, use a good camera. If you want to record EVP, use a good audio recorder. And leave the Kinect / X Box home to play games on it. You will get less data that way, but what you do get stands a much better chance of being something worth looking at.
Visit my website, "The Paranormal DESTINATION"
Posted 18 September 2013 - 05:48 AM
Posted 17 October 2013 - 05:39 PM
Caverat, I never thought of it that way, that is a pretty good view on the whole thing.
Hope you all are doing well.
Posted 04 June 2014 - 08:16 AM
For the add, they weren't using an Xbox Kinect, but was using a custom software that utilizes motion sensors and there's no way that it could be used as Xbox marketing when they never even mention Xbox or Xbox Kinect, they claim it as their engineer's custom software that he developed, and I feel it's a good idea to experiment with different devices in investigation to get evidence through a broad variety of research not to mention when one device may fail to capture for certain pieces of evidence another may succeed and via verse.
Posted 04 June 2014 - 04:01 PM
The same applies to all "new" methods someone proposes..... Test them against known science. Of course a new application may be untested at first but it doesn't take much to start the evaluation process. To begin, does it build on something we already know? Most science does, it takes something common and uses it in a different way. Second does this "different way" make sense? Is it explainable? Science works on the basis of proving theories, so even a new method should be accompanied by a theory, that is someone explaining how it works, or should work. Read the theory behind it, if it makes no sense or requires a lot of mumbo jumbo, discard it. On the other hand if it makes sense and could actually work then go on. Test the theory. In the end it may fall apart, but it also may just move things forward.
Visit my website, "The Paranormal DESTINATION"
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Financial Finesse's latest report focuses on the gap in financial wellness between men and women. Women significantly lag men in basic money management and investing, the report found. Not only that, but the gender gap is actually widening in some areas.
Financial Finesse analyzed questions that employees put to the firm's financial hotline from January through March, and broke down the data into seven topics: investing, money management, tax planning, college planning, insurance, retirement planning and estate planning.
Nearly 90% of men claim to have general investment knowledge, but only 66% of women could agree. While men reported relatively low levels of confidence in their allocations (45%), it far exceeded the 29% of women who said they felt confident.
Furthermore, it seems very few women have reviewed their plans to make sure everything is in order. Just 37% said they had taken a risk assessment and were aware of where they sat on the risk tolerance spectrum, a full 20 percentage points less than men who have done the same. While 38% of men have reviewed their assets and created a master plan to avoid overweighting certain asset classes or overlapping investments, just 16% of women have, and only 10% of women have done a fee analysis on their portfolio. Twenty-eight percent of men have done a fee analysis.
The gap between men and women with a general knowledge of investment products is noticeably growing, the report found. In 2010, the gap was 33 percentage points, a gap that more than halved in 2011. This year, though, the gap increased to 23 percentage points.
In money management, the gap between men and women was also significant. Debt is clearly a problem for women. Fifty-two percent say they are comfortable with the amount of non-mortgage debt they have, and only 45% regularly pay their full credit card balance. Less than half check their credit report on a regular basis, compared with 58% of men.
"It seems like men and women have taken a step backward," Diane Winland, resident financial planner at Financial Finesse, told AdvisorOne on Thursday. "Generally, men are pulling back across the board, and women are pulling back in certain areas like money management and investing. We're seeing a bigger pullback in the day-to-day, but none in retirement."
The report notes that between 2009 and 2011, women made "steady progress" in paying off credit card debt in full, before reversing course in the first quarter. "This, coupled with the steady decline in the percentage of women that report feeling comfortable with their level of non-mortgage debt, may be cause for concern and is something worth watching in the future," according to the report.
Regarding taxes, women are struggling to understand the implications of their investments and retirement accounts. Just 34% said they understand the subject, compared with 56% of men.
Winland (left) stressed the importance of understanding the difference between Roth IRAs and traditional IRAs. It's also important, she said, to understand how distributions are taxed as they come out of the account.
Women reported low levels of understanding in college planning, but this subject also presents the only area in the report where women outpaced men. Less than 30% of men know how much they need to save for college costs and are on track to meet that goal, but only 16% of women agreed. Over a third of men are saving for college in a tax-advantaged plan, compared with 19% of women. Women edged out men in understanding financial aid options, but just barely; 60% of women understand grants, scholarships and loans, compared with 59% of men.
When it comes to insurance, the gap between men and women begins to shrink, with both groups reporting fairly high levels of understanding in some areas. Seventy-eight percent of women review their insurance coverage every year and are confident they are adequately covered, compared with 82% of men. Half of men and 42% of women report having enough life insurance. While both groups are prepared for a long-term disability (53% of men have long-term disability coverage, compared with 52% of women), long-term care insurance is still something of a novelty. Just 16% of men reported having coverage, compared with 12% of women.
The good news is that in retirement planning, women aren't lagging men as badly as they are in other areas. In fact, the report found "virtually no gap" between men and women participating in 401(k)s or IRAs. Almost 80% of women are taking full advantage of their company match, and 87% of men are doing likewise. Unfortunately, neither group appears well-prepared for retirement. After falling from 14 percentage points in 2010 to just eight points in 2011, the gap between men and women who are on track for retirement increased in 2012. Just 22% of men said they are on target to replace 80% of their income in retirement. A piddly 12% of women said they were on track.
If men and women are already participating in their company's retirement plan and contributing the max to get the full match, what else can they do to improve their retirement chances? One thing, Winland said, is to put in the actual maximum they are allowed contribute to the plan, and not just the maximum needed to earn the company match. "Planners like to see people putting in 10%," she said. "But for some people the max to get the match may only by 6%." Workers need to "set a higher bar for how much they're going to save for retirement," Winland said. "Once they've maxed out their contribution, maybe they can supplement that with an IRA."
The subject of estate planning, while it didn't represent the wide gap other topics did, also indicated a challenge for men and women. Although 81% of men and 80% of women have updated their beneficiary designations, less than 5% of each group has met with a professional to create an estate plan.
Winland was disappointed in women's apparent backslide after improvements in 2011. "I would have thought women would continue gaining ground," she said of this year's report. She noted that men are pulling back, but women are pulling back more, and wondered if women were more reactive than men. "It seems women are more sensitive to outside changes," she said.


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A stage hypnotist from Yorkshire burst into the Sunday papers this weekend after it emerged that Britain's ladies are using his self-hypnosis CDs to increase their breast size by as much as two cups.
David Knight came onto the papers' radar when the BBC issued a press release ahead of a documentary it claims "shows how easy it is to set yourself up as an "accredited" hypnotherapist with absolutely no qualifications".
According to the Beeb, Knight "quotes an 85 per cent success rate. He tells Inside Out that his figure is based on his own customer feedback and a study of 22 women carried out in the Seventies."
He says, the Beeb claims: "Every lady's breasts today have been grown by the mind. The mind grows it, so the mind can enhance it. Fact."
However, Knight displays the evidence here on his website [scientific but arguably not safe for work] where he cites a study which showed results of as much as two inches' worth of bust improvement.
He cites one happy customer's testimonial: "I have been using them faithfully for about three weeks now and have grown one inch already!!! ... The other thing is, I have lost two inches around my waist, however I have also been working out quite a bit as well! ...I just had to share my success with you, no one else would believe me (except my husband) Thank You so much for your good work. You are just GREAT!!!"
It's not just the ladies who can benefit from Knight's gift though. He also claims his CDs can put martial artists "in the perfect zone to complete your task and beat any competitor. With the power of Hypnosis you will always perform at your best with a sharp and clear mind."
Is there nothing that won't succumb to hypnosis?
Not much it seems, with Knight claiming success against phobias, smoking and flagging sex drives.
Even irritable bowel syndrome will succumb, as his CDs will alleviate the stress that is often behind the crippling condition. But Knight admits even hypnosis has its limits, warning: "Rectal bleeding is never caused by IBS, and any rectal bleeding must be properly and thoroughly evaluated." ®


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Scheinman Institute on Conflict Resolution
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"Educating the Next Generation of Neutrals and Practitioners"
The Scheinman Institute on Conflict Resolution at Cornell University promotes interdisciplinary education, research and training in dispute resolution for students, academics, neutrals and practitioners.
The Institute builds on the Cornell ILR School's proud tradition of instruction in the practical use of conflict resolution skills in the workplace and beyond.
In support of its mission, the Scheinman Institute's principal activities involve:
- Undergraduate and graduate courses and degrees
- Training and certificate programs for neutrals and professionals
- Consultation and technical assistance to practitioners and neutrals
- Scholarly and empirical research and evaluation
- Conferences, symposia and workshops
In Cornell's ILR School, the Institute continues its focus on programs dealing with workplace dispute resolution. Through its joint program with the Cornell Law School and relationships with the other Cornell colleges, the Institute addresses commercial, environmental, international, regulatory and other conflicts that arise in the public, private and non-profit sectors as well as in complex litigation where creative conflict resolution can have a meaningful impact.
In all of its activities, the Institute is committed to promoting diversity, cross-cultural understanding and equal opportunities for individuals without regard to race, gender, religion, national origin, sexual orientation, age, disability or other protected characteristics.