text:
Death Approaching
Most Relevant Verses
He said, See here now; I am old, I do not know when I may die.
And the Lord said to Moses, Behold, your days are nearing when you must die. Call Joshua and present yourselves at the Tent of Meeting, that I may give him his charge. And Moses and Joshua went and presented themselves at the Tent of Meeting.
I saw in the night visions, and behold, on the clouds of the heavens came One like a Son of man, and He came to the Ancient of Days and was presented before Him.
When the tabernacle is to go forward, the Levites shall take it down, and when the tabernacle is to be pitched, the Levites shall set it up. And the excluded [any not of the tribe of Levi] who approach the tabernacle shall be put to death.
So Bathsheba went in to the king in his chamber. Now the king was very old and feeble, and Abishag the Shunammite was ministering to [him].
When David was old and full of days, he made Solomon his son king over Israel.
Believe Me when I assure you, most solemnly I tell you, the time is coming and is here now when the dead shall hear the voice of the Son of God and those who hear it shall live.
When David's time to die was near, he charged Solomon his son, saying,
So Joseph died, being 110 years old; and they embalmed him, and he was put in a coffin in Egypt.
Now when the time was almost come for Jesus to be received up [to heaven], He steadfastly and determinedly set His face to go to Jerusalem.
Do not be surprised and wonder at this, for the time is coming when all those who are in the tombs shall hear His voice,
And Joseph said to his brethren, I am going to die. But God will surely visit you and bring you out of this land to the land He swore to Abraham, to Isaac, and to Jacob [to give you].
For You have delivered my life from death, my eyes from tears, and my feet from stumbling and falling.
Bible Theasaurus
- Approaching (73 instances)


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text:
Biomed News
on Polyamines
Issue of 2019‒06‒09
seventeen papers selected by
Alexander Ivanov
Engelhardt Institute of Molecular Biology
Discovery and antitumor evaluation of novel inhibitors of spermine oxidase.
New coordination compounds of citric acid and polyamines with lanthanide ions - potential application in monitoring the treatment of cancer diseases.
MYC-driven small cell lung cancer is metabolically distinct and vulnerable to arginine depletion.
The C-terminal flexible region of branched-chain polyamine synthase facilitates substrate specificity and catalysis.
Spermidine strongly increases the fidelity of Escherichia coli CRISPR Cas1-Cas2 integrase.
Absolute requirement for polyamines for growth of Escherichia coli mutants (mnmE/G) defective in modification of the wobble anticodon of transfer-RNA.
A metabolic profile of polyamines in Parkinson's disease: a promising biomarker.
Amniculibacterium aquaticum gen. nov., sp. nov., a new member of the family Flavobacteriaceae isolated from a stream.
Polyamines modulate growth, antioxidant activity, and genomic DNA in heavy metal-stressed wheat plant.
Flavobacterium stagni sp. nov., isolated from a freshwater reservoir.
Molecular Targets for Malarial Chemotherapy: A Review.
Inhella crocodyli sp. nov., isolated from a crocodile pond.
Hymenobacter oligotrophus sp. nov., isolated from a contaminated agar plate.
Arginase II activity regulates cytosolic Ca2+ level in a p32-dependent manner that contributes to Ca2+-dependent vasoconstriction in native low-density lipoprotein-stimulated vascular smooth muscle cells.
Sphingobium terrigena sp. nov., isolated from gasoline-contaminated soil.
Chakrabartia godavariana gen. nov., sp. nov., a novel member of the family Sphingomonadaceae isolated from the Godavari River, India.
Disturbance of I1-imidazoline receptor signal transduction in cardiomyocytes of Spontaneously Hypertensive Rats.
J Enzyme Inhib Med Chem. 2019 Dec;34(1): 1140-1151
Discovery and antitumor evaluation of novel inhibitors of spermine oxidase.
Sun L
Yang J
Qin Y
Wang Y
Wu H
Zhou Y
Cao C
Increasing knowledge of the relationship between cancer and dysregulated polyamine catabolism suggests interfering with aberrant polyamine metabolism for anticancer therapy that will have considerable clinical promise. SMO (spermine oxidase) plays an essential role in regulating the polyamines homeostasis. Therefore, development of SMO inhibitors has increasingly attracted much attention. Previously, we successfully purified and characterised SMO. Here, we presented an in silico drug discovery pipeline by combining pharmacophore modelling and molecular docking for the virtual screening of SMO inhibitors. In vitro evaluation showed that N-(3-{[3-(dimethylamino)propyl]amino}propyl)-8-quinolinecarboxamide (SI-4650) inhibited SMO enzyme activity, increased substrate spermine content and reduced product spermidine content, indicating that SI-4650 can interfere with polyamine metabolism. Furthermore, SI-4650 treatment suppressed cell proliferation and migration. Mechanistically, SI-4650 caused cell cycle arrest, induced cell apoptosis, and promoted autophagy. These results demonstrated the properties of interfering with polyamine metabolism of SI-4650 as a SMO inhibitor and the potential for cancer treatment.
Quinolinecarboxamide; antitumor activity; spermine oxidase
J Inorg Biochem. 2019 May 17. pii: S0162-0134(19)30093-5. [Epub ahead of print]198 110715
New coordination compounds of citric acid and polyamines with lanthanide ions - potential application in monitoring the treatment of cancer diseases.
Zabiszak M
Nowak M
Gabryel M
Ogawa K
Kaczmarek MT
Hnatejko Z
Jastrzab R
Non-covalent interaction in the binary systems of polyamines (putrescine, spermidine, spermine) with citric acid and complex formation in the binary as well as ternary systems of lanthanide(III) ions, citric acid and polyamine have been investigated. The studies were performed in aqueous solution. The overall stability constants of the complexes were determined using the potentiometric method with computer analysis of the data. Only mononuclear type of complexes were found in the ternary systems and polyamines were located in the outer as well as inner coordination sphere. Non-covalent interaction between biogenic amines and citric acid in the binary and ternary systems were confirmed on the basis of the equilibrium constants analysis and spectroscopic studies.
Biogenic amines; Citric acid; F-electron complexes; Non-covalent interactions; Potentiometric measurements; Spectroscopic studies
Clin Cancer Res. 2019 Jun 04. pii: clincanres.4140.2018. [Epub ahead of print]
MYC-driven small cell lung cancer is metabolically distinct and vulnerable to arginine depletion.
Chalishazar MD
Wait SJ
Huang F
Ireland AS
Mukhopadhyay A
Lee Y
Schuman S
Guthrie MR
Berrett K
Vahrenkamp J
Hu Z
Kudla M
Modzelewska K
Wang G
Ingolia NT
Gertz J
Lum DH
Cosulich SC
Bomalaski JS
DeBerardinis RJ
Oliver TG
: Small cell lung cancer (SCLC) has been treated clinically as a homogeneous disease, but recent discoveries suggest that SCLC is heterogeneous. Whether metabolic differences exist among SCLC subtypes is largely unexplored. In this study, we aimed to determine whether metabolic vulnerabilities exist between SCLC subtypes that can be therapeutically exploited.
: We performed steady state metabolomics on tumors isolated from distinct GEMMs representing the MYC and MYCL-driven subtypes of SCLC. Using genetic and pharmacological approaches, we validated our findings in chemo-naive and resistant human SCLC cell lines, multiple GEMMs, four human cell line xenografts, and four newly-derived PDX models.
: We discover that SCLC subtypes driven by different MYC family members have distinct metabolic profiles. MYC-driven SCLC preferentially depends on arginine-regulated pathways including polyamine biosynthesis and mTOR pathway activation. Chemo-resistant SCLC cells exhibit increased MYC expression and similar metabolic liabilities as chemo-naive MYC-driven cells. Arginine depletion with pegylated arginine deiminase (ADI-PEG 20) dramatically suppresses tumor growth and promotes survival of mice specifically with MYC-driven tumors, including in GEMMs, human cell line xenografts, and a PDX from a relapsed patient. Finally, ADI-PEG 20 is significantly more effective than the standard of care chemotherapy.
: These data identify metabolic heterogeneity within SCLC and suggest arginine deprivation as a subtype-specific therapeutic vulnerability for MYC-driven SCLC.
FEBS J. 2019 Jun 04.
The C-terminal flexible region of branched-chain polyamine synthase facilitates substrate specificity and catalysis.
Hidese R
Toyoda M
Yoshino KI
Fukuda W
Wihardja GA
Kimura S
Fujita J
Niitsu M
Oshima T
Imanaka T
Mizohata E
Fujiwara S
Branched-chain polyamine synthase (BpsA) catalyzes sequential aminopropyl transfer from the donor, decarboxylated S-adenosylmethionine (dcSAM), to the acceptor, linear-chain polyamine, resulting in the production of a quaternary-branched polyamine via tertiary branched polyamine intermediates. Here, we analyzed the catalytic properties and X-ray crystal structure of Tth-BpsA from Thermus thermophilus and compared them with those of Tk-BpsA from Thermococcus kodakarensis, which revealed differences in acceptor substrate specificity and C-terminal structure between these two enzymes. To investigate the role of the C-terminal flexible region in acceptor recognition, a region (LQDEEATTY) in Tth-BpsA was replaced with that in Tk-BpsA (YDDEESSTT) to create chimeric Tth-BpsA C9, which showed a severe reduction in catalytic efficiency toward N4 -aminopropylnorspermidine, but not toward N4 -aminopropylspermidine, mimicking Tk-BpsA substrate specificity. Tth-BpsA C9 Tyr346 and Thr354 contributed to discrimination between tertiary branched-chain polyamine substrates, suggesting that the C-terminal region of BpsA recognizes acceptor substrates. Liquid chromatography-tandem mass spectrometry analysis on a Tk-BpsA reaction mixture with dcSAM revealed two aminopropyl groups bound to two of five aspartate/glutamate residues (Glu339 , Asp342 , Asp343 , Glu344 , and Glu345 ) in the C-terminal flexible region. Mutating each of these five amino acid residues to asparagine/glutamine resulted in a slight decrease in activity. The quadruple mutant D342N/D343N/E344Q/E345Q exhibited a severe reduction in catalytic efficiency, suggesting that these aspartate/glutamate residues function to receive aminopropyl chains. In addition, the X-ray crystal structure of the Tk-BpsA ternary complex bound to N4 -bis(aminopropyl)spermidine revealed that Asp126 and Glu259 interacted with the aminopropyl moiety in N4 -aminopropylspermidine. This article is protected by copyright. All rights reserved.
Branched-chain polyamine; Thermophile; crystal structure; enzyme mechanism
J Biol Chem. 2019 Jun 06. pii: jbc.RA119.007619. [Epub ahead of print]
Spermidine strongly increases the fidelity of Escherichia coli CRISPR Cas1-Cas2 integrase.
Plateau P
Moch C
Blanquet S
Site-selective CRISPR array expansion at the origin of bacterial adaptive immunity relies on recognition of sequence-dependent DNA structures by the conserved Cas1-Cas2 integrase. Off-target integration of a new spacer sequence outside canonical CRISPR arrays has been described in vitro However, this nonspecific integration activity is rare in vivo Here, we designed gel assays to monitor fluorescently labeled protospacer insertion in a supercoiled 3-kb plasmid harboring a minimal CRISPR locus derived from the Escherichia coli type I-E system. This assay enabled us to distinguish and quantify target and off-target insertion events catalyzed by E. coli Cas1-Cas2 integrase. We show that addition of the ubiquitous polyamine spermidine or of another polyamine, spermine, significantly alters the ratio between target and off-target insertions. Notably, addition of 2 mM spermidine quenched the off-target spacer insertion rate by a factor of 20-fold, and, in the presence of integration host factor (IHF), spermidine also increased insertion at the CRISPR locus 1.5-fold. The observation made in our in vitro system that spermidine strongly decreases nonspecific activity of Cas1-Cas2 integrase outside the leader-proximal region of a CRISPR array suggests that this polyamine plays a potential role in the fidelity of the spacer integration also in vivo.
CRISPR/Cas; DNA-protein interaction; Integration Host Factor (IHF); genomic instability; integrase; polyamine
FEMS Microbiol Lett. 2019 Jun 04. pii: fnz110. [Epub ahead of print]
Absolute requirement for polyamines for growth of Escherichia coli mutants (mnmE/G) defective in modification of the wobble anticodon of transfer-RNA.
Keller C
Chattopadhyay M
Tabor H
The genes mnmE and mnmG are responsible for the modification of uridine 34, 'the wobble position' of many aminoacyl-tRNAs. Deletion of these genes affects the strength of the codon-anticodon interactions of the aminoacyl-tRNAs with the mRNAs and the ribosomes. However, deletion of these genes does not usually have a significant effect on the growth rate of the standard Escherichia coli strains. In contrast we have found that if the host E. coli strain is deficient in the synthesis of polyamines, deletion of the mnmE or mnmG gene results in complete inhibition of growth unless the medium contains polyamines. The finding of an absolute requirement for polyamines in our current work will be significant in studies on polyamine function, in studies on the function of the mnmE/G genes, and in studies on the role of aminoacyl-tRNAs in protein biosynthesis.
Gene mnmE; aminoacyl-tRNA; mRNA; polyamines; putrescine; spermidine
Ann Neurol. 2019 Jun 02.
A metabolic profile of polyamines in Parkinson's disease: a promising biomarker.
Saiki S
Sasazawa Y
Fujimaki M
Kamagata K
Kaga N
Taka H
Li Y
Souma S
Hatano T
Imamichi Y
Furuya N
Mori A
Oji Y
Ueno SI
Nojiri S
Miura Y
Ueno T
Funayama M
Aoki S
Hattori N
: Aging is the highest risk factor for Parkinson's disease (PD). Under physiological conditions, spermidine and spermine experimentally enhance longevity via autophagy induction. Accordingly, we evaluated the ability of each polyamine metabolite to act as age-related, diagnostic and severity-associated PD biomarkers.
: Comprehensive metabolome analysis of plasma was performed in Cohort A (controls: 45, PD: 145), followed by analysis of seven polyamine metabolites in Cohort B (controls: 49, PD: 186, progressive supranuclear palsy: 19, Alzheimer's disease: 23). Furthermore, 20 patients with PD who were successively examined within Cohort B were studied using diffusion tensor imaging (DTI). Association of each polyamine metabolite with disease severity was assessed according to Hoehn and Yahr stage (H&Y) and Unified Parkinson's Disease Rating Scale motor section (UPDRS-III). Additionally, the autophagy induction ability of each polyamine metabolite was examined in vitro in various cell lines.
: In Cohort A, N8-acetylspermidine and N-acetylputrescine levels were significantly and mildly elevated in PD, respectively. In Cohort B, spermine levels and spermine/spermidine ratio were significantly reduced in PD, concomitant with hyperacetylation. Furthermore, N1,N8-diacetylspermidine levels had the highest diagnostic value, and correlated with H&Y, UPDRS-III, and axonal degeneration quantified by DTI. The spermine/spermidine ratio in controls declined with age, but was consistently suppressed in PD. Among polyamine metabolites, spermine was the strongest autophagy inducer, especially in SH-SY5Y cells. No significant genetic variations in five genes encoding enzymes associated with spermine/spermidine metabolism, were detected compared with controls.
: Spermine synthesis and N1,N8-diacetylspermidine may respectively be useful diagnostic and severity-associated biomarkers for PD. This article is protected by copyright. All rights reserved.
N1,N8-diacetylspermidine; Parkinson's disease; autophagy; biomarker; spermine
Arch Microbiol. 2019 Jun 04.
Amniculibacterium aquaticum gen. nov., sp. nov., a new member of the family Flavobacteriaceae isolated from a stream.
Chen WM
Guo YP
Sheu C
Sheu SY
Strain KYPW7T, isolated from the Funglin Stream in Taiwan, was characterized using a polyphasic taxonomy approach. Cells of strain KYPW7T were Gram-stain-negative, aerobic, non-spore forming, non-motile rods and formed white colonies. Growth occurred at 15-30 °C (optimum 25 °C), at pH 6-8 (optimum pH 6.5) and with 0-1% NaCl (optimum 0%). Phylogenetic analyses based on 16S rRNA and coding sequences of 92 protein clusters showed that strain KYPW7T represents a novel genus in the family Flavobacteriaceae. The 16S rRNA gene sequence of strain KYPW7T was related to the species of the genera Chryseobacterium (91.8-96.0% sequence similarity), Bergeyella (95.1-95.8%), Cloacibacterium (94.5-95.7%), Daejeonia (95.6%) and Riemerella (94.0-95.0%). Strain KYPW7T showed less than 72% average nucleotide identity and less than 24% digital DNA-DNA hybridization identity compared to the type strains of related genera within the family Flavobacteriaceae. The predominant fatty acids were iso-C15:0 and iso-C17:0 3-OH. The major isoprenoid quinone was MK-6 and the DNA G + C content was 36.8 mol%. The polar lipids had phosphatidylethanolamine, three uncharacterized aminophospholipids and an uncharacterized phospholipid. The polyamines contained homospermidine, putrescine and spermidine. On the basis of the genotypic and phenotypic data, strain KYPW7T represents a novel species of a new genus in the family Flavobacteriaceae, for which the name Amniculibacterium aquaticum gen. nov., sp. nov. is proposed. The type strain is KYPW7T (= BCRC 81123T = LMG 30598T = KCTC 62512T).
Amniculibacterium aquaticum gen. nov., sp. nov.; Bacteroidetes; Flavobacteriaceae; Flavobacteriales; Flavobacteriia; Polyphasic taxonomy
Environ Sci Pollut Res Int. 2019 Jun 01.
Polyamines modulate growth, antioxidant activity, and genomic DNA in heavy metal-stressed wheat plant.
Taie HAA
Seif El-Yazal MA
Ahmed SMA
Rady MM
A pot experiment was performed to assess the useful effects of seed soaking or seedling foliar spray using 0.25 mM spermine (Spm), 0.50 mM spermidine (Spd), or 1 mM putrescine (Put) on heavy metal tolerance in wheat plants irrigated with water contaminated by cadmium (2 mM Cd2+ in CdCl2) or lead (2 mM Pb2+ in PbCl2). Cd2+ or Pb2+ presence in the growth medium resulted in significant reductions in growth and yield characteristics and activities of leaf peroxidase (POD), glutathione reductase (GR), ascorbic acid oxidase (AAO), and polyphenol oxidase (PPO) of wheat plants. In contrast, significant increases were observed for Cd2+ content in roots, leaves and grains, superoxide dismutase (SOD) and catalase (CAT) activities, radical scavenging activity (DPPH), reducing power capacity, and fragmentation in DNA in comparison to controls (without Cd2+ or Pb2+ addition). However, treating the Cd2+- or Pb2+-stressed wheat plants with Spm, Spd, or Put, either by seed soaking or foliar spray, significantly improved growth and yield characteristics and activities of POD, GR, AAO, PPO, SOD, and CAT, DPPH, and reducing power capacity in wheat plants. In contrast, Cd2+ levels in roots, leaves, and yielded grains, and fragmentation in DNA were significantly reduced compared with the stressed (with Cd2+ or Pb2+) controls. Generally, seed soaking treatments were more effective than foliar spray treatments. More specifically, seed priming in Put was the best treatment under heavy metal stress. Results of this study recommend using polyamines, especially Put, as seed soaking to relieve the adverse effects of heavy metals in wheat plants.
Antioxidant activity; Antioxidant enzymes; Cadmium; Genomic DNA; Growth and productivity; Lead; Polyamines; Wheat
Int J Syst Evol Microbiol. 2019 Jun 03.
Flavobacterium stagni sp. nov., isolated from a freshwater reservoir.
Chen WM
Guo YP
Kwon SW
Sheu C
Sheu SY
Strain WWJ-16T was isolated from a freshwater reservoir in Taiwan. Phylogenetic analyses based on 16S rRNA gene sequences and an up-to-date bacterial core gene set (92 protein clusters) indicated that strain WWJ-16T is affiliated with the species in the genus Flavobacterium. Strain WWJ-16T was most closely related to Flavobacterium fontis MIC3010T (97.9 % 16S rRNA gene sequence identity) and Flavobacterium squillarum CMJ-5T (97.6 %). Strain WWJ-16T showed 77.4 % average nucleotide identity and 20.6 % digital DNA-DNA hybridization identity with F. fontis MIC3010T. Cells of strain WWJ-16T were Gram-stain-negative, strictly aerobic, non-motile, rod-shaped and formed yellow colonies. Optimal growth occurred at 25 °C, pH 7 and in 0.5-1 % NaCl (w/v). Strain WWJ-16T contained iso-C15 : 1 G and iso-C15 : 0 as predominant fatty acids. The major hydroxyl fatty acids were iso-C15 : 0 3-OH, iso-C17 : 0 3-OH and iso-C16 : 0 3-OH. The polar lipid profile consisted of phosphatidylethanolamine, four uncharacterized aminophospholipids and four uncharacterized phospholipids. The major polyamine was homospermidine. The major isoprenoid quinone was MK-6. The genomic DNA G+C content of strain WWJ-16T was 39.4 mol%, as determined by genome sequencing. The genotypic and phenotypic characteristics indicate that strain WWJ-16T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium stagni sp. nov. is proposed. The type strain is WWJ-16T (=BCRC 81125T =LMG 30600T=KCTC 62515T).
Curr Top Med Chem. 2019 Jun 02.
Molecular Targets for Malarial Chemotherapy: A Review.
Yadav DK
Kumar S
Teli MK
Yadav R
Chaudhary S
The malaria parasite resistance to the existing drugs is a serious problem to currently used anti-malarials and, thus, highlights the urgent need to develop new and effective anti-malarial molecules. This could be achieved either by the identification of the new drugs for the validated targets or by further refining/improving the existing antimalarials; or by combining previously effective agents with new/existing drugs to have synergistic effect that counter parasite resistance; or by identifying novel targets for the malarial chemotherapy. In this review article, a comprehensive collection of some of the novel molecular targets has been enlisted for the antimalarial drugs. The targets which could be deliberated for developing new anti-malarial drugs could be: membrane biosynthesis, mitochondrial system, apicoplasts, parasite transporters, shikimate pathway, hematin crystals, parasite proteases, glycolysis, isoprenoid synthesis, cell cycle control/cycline dependent kinase, redox system, nucleic acid metabolism, methionine cycle and the polyamines, folate metabolism, the helicases, erythrocyte G-protein, and farnesyl transferases. Modern genomic tools approaches such as structural biology and combinatorial chemistry, novel targets could be identified followed by drug development for drug resistant strains provide wide ranges of novel targets in the development of new therapy. The new approaches and targets mentioned in the manuscript provide a basis for the development of new unique strategies for antimalarial therapy with limited off- target effects in the near future.
Cyclin-dependent kinases; Drug targets; Helicases; Parasite proteases; Plasmodium falciparum; anti-malaria; drug development
Int J Syst Evol Microbiol. 2019 Jun 03.
Inhella crocodyli sp. nov., isolated from a crocodile pond.
Chen WM
Xie YR
Young CC
Sheu SY
Strain CCP-18T, isolated from a freshwater pond in Taiwan, was characterized by using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain CCP-18T belongs to the genus Inhella and has the highest levels of sequence similarity with respect to Inhella inkyongensis IMCC1713T (98.9 %) and Inhella fonticola TNR-25T (98.0 %). Cells were Gram-stain-negative, aerobic, motile, rod-shaped and formed white-coloured colonies. Optimal growth occurred at 25 °C, pH 6 and in the absence of NaCl. The major fatty acids of strain CCP-18T were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unidentified aminophospholipids, an unidentified phospholipid, an unidentified aminolipid and an unidentified lipid. The predominant polyamine was putrescine. The major isoprenoid quinone was Q-8. The draft genome was approximately 3.76 Mb in size with a G+C content of 68.9 mol%. The DNA-DNA hybridization values for strain CCP-18Twith I.inkyongensis IMCC1713T and I.nhella fonticola TNR-25T were less than 40 %. Based on the phylogenetic and phenotypic data, strain CCP-18T should be classified within the genus Inhella as a representative of a novel species, named Inhella crocodyli sp. nov. The type strain is CCP-18T (=BCRC 81120T=LMG 30595T=KCTC 62511T).
Antonie Van Leeuwenhoek. 2019 Jun 04.
Hymenobacter oligotrophus sp. nov., isolated from a contaminated agar plate.
Geng Y
Zhang Y
Tian J
Liu J
Qin K
Huang Y
Wei Z
Peng F
A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6T, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory was performed. Its optimum growth temperature was determined to be 28 °C in the absence of NaCl on R2A plates. On the basis of 16S rRNA gene sequence analysis, strain sh-6T belongs to the genus Hymenobacter and is closely related to Hymenobacter deserti ZLB-3T (95.05%), Hymenobacter paludis KBP-30T (94.96%), Hymenobacter coalescens WW84T (94.04%), Hymenobacter gummosus ANT-18T (93.38%), Hymenobacter ocellatus Myx2105T (93.70%), Hymenobacter jeollabukensis 1-3-3-8T (93.48%) and Hymenobacter koreensis GYR3077T (93.21%). Comparison of the genome of strain sh-6T and that of H. gummosus ANT-18T gave digital DNA-DNA hybridization and Average Nucleotide Identity values of 20.6% and 78.4%, respectively. The respiratory isoprenoid quinone and polyamine component were identified as MK-7 and sym-homospermidine, respectively. The major cellular fatty acids identified as iso-C15:0, summed feature 4 (iso-C17:1 I/anteiso B), iso-C16:0, iso-C17:0 3-OH and iso-C17:0. The major polar lipid of strain sh-6T determined to be phosphatidylethanolamine. The DNA G+C content was determined to be 60.5 mol%. On the basis of the evidence presented in this study, a novel species of the genus Hymenobacter, Hymenobacter oligotrophus sp. nov., is proposed, with the type strain sh-6T (= CCTCC AB 2016064T = KCTC 62345T).
Hymenobacter oligotrophus; Novel species; Phylogenetic analysis; Taxonomy
Exp Mol Med. 2019 Jun 03. 51(6): 60
Arginase II activity regulates cytosolic Ca2+ level in a p32-dependent manner that contributes to Ca2+-dependent vasoconstriction in native low-density lipoprotein-stimulated vascular smooth muscle cells.
Koo BH
Hong D
Hong HD
Lim HK
Hoe KL
Won MH
Kim YM
Berkowitz DE
Ryoo S
Although arginase II (ArgII) is abundant in mitochondria, Ca2+-accumulating organelles, the relationship between ArgII activity and Ca2+ translocation into mitochondria and the regulation of cytosolic Ca2+ signaling are completely unknown. We investigated the effects of ArgII activity on mitochondrial Ca2+ uptake through mitochondrial p32 protein (p32m) and on CaMKII-dependent vascular smooth muscle cell (VSMC) contraction. Native low-density lipoprotein stimulation induced an increase in [Ca2+]m as measured by CoCl2-quenched calcein-AM fluorescence, which was prevented by Arg inhibition in hAoSMCs and reduced in mAoSMCs from ArgII-/- mice. Conversely, [Ca2+]c analyzed with Fluo-4 AM was increased by Arg inhibition and ArgII gene knockout. The increased [Ca2+]c resulted in CaMKII and MLC 20 phosphorylation, which was associated with enhanced vasoconstriction activity to phenylephrine (PE) in the vascular tension assay. Cy5-tagged siRNA against mitochondrial p32 mRNA (sip32m) abolished mitochondrial Ca2+ uptake and induced activation of CaMKII. Spermine, a polyamine, induced mitochondrial Ca2+ uptake and dephosphorylation of CaMKII and was completely inhibited by sip32m incubation. In mAoSMCs from ApoE-null mice fed a high-cholesterol diet (ApoE-/- +HCD), Arg activity was increased, and spermine concentration was higher than that of wild-type mice. Furthermore, [Ca2+]m and p32m levels were elevated, and CaMKII phosphorylation was reduced in mAoSMCs from ApoE-/- +HCD. In vascular tension experiments, an attenuated response to vasoconstrictors in de-endothelialized aorta from ApoE-/- +HCD was recovered by incubation of sip32m. ArgII activity-dependent production of spermine augments Ca2+ transition from the cytosol to the mitochondria in a p32m-dependent manner and regulates CaMKII-dependent constriction in VSMCs.
Int J Syst Evol Microbiol. 2019 Jun 06.
Sphingobium terrigena sp. nov., isolated from gasoline-contaminated soil.
Park YJ
Kim KH
Han DM
Lee DH
Jeon CO
A Gram-stain-negative, strictly aerobic bacterial strain, designated EO9T, was isolated from gasoline-contaminated soil in the Republic of Korea. Cells were non-motile short rods showing catalase- and oxidase-positive reactions. Growth was observed at 10-37 °C (optimum, 30 °C), at pH 6.0-9.0 (pH 6.5) and in the presence of 0-0.5 % (w/v) NaCl (0 %). Ubiquinone-10 (Q-10) and spermidine were identified as the predominant respiratory quinone and polyamine, respectively. Summed feature 8 (comprising C18:1ω7c/C18:1ω6c), summed feature 3 (comprising C16:1ω7c/C16:1ω6c), C16:0 and C14:0 2-OH were identified as major cellular fatty acids. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid, phosphatidylglycerol and an unidentified phospholipid. The G+C content of the genomic DNA was 62.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain EO9T formed a tight phylogenetic lineage with Sphingobium xenophagum NBRC 107872T and Sphingobium hydrophobicum C1T within the genus Sphingobium. Strain EO9T was most closely related to S. xenophagum NBRC 107872T (97.2 %) and S. hydrophobicum C1T (97.2 %), but DNA-DNA relatedness levels between strain EO9T and the type strains of S. xenophagum and S. hydrophobicum were 37.1 and 36.8 % , respectively. Based on its phenotypic, chemotaxonomic and molecular features, strain EO9T clearly represents a novel species of the genus Sphingobium, for which the name Sphingobium terrigena sp. nov. is proposed. The type strain is EO9T (=KACC 19523T=JCM 32762T).
Int J Syst Evol Microbiol. 2019 Jun 05.
Chakrabartia godavariana gen. nov., sp. nov., a novel member of the family Sphingomonadaceae isolated from the Godavari River, India.
Jani K
Feng GD
Zhu HH
Prakash O
Bandal J
Rale V
Shouche Y
Sharma A
A Gram-stain-negative, aerobic, yellow-pigmented, oxidase-positive and rod-shaped bacterium, designated PRB40T, was isolated from the Godavari River in India during the course of 'Kumbh Mela', the world's largest mass gathering event. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain PRB40T formed a lineage within the family Sphingomonadaceae and was distinct from the most closely related genera Sphingorhabdus, Novosphingobiumand Sphingomonas with sequence similarity values ≤95.2 %. Growth of strain PRB40T occurred at 10-40 °C (optimum 30 °C), at pH 6.0-9.0 (pH 7.0) and with 0-0.5 % (w/v) NaCl concentration (0 %). The major respiratory quinone was ubiquinone-10 (Q-10). It contained C17 : 1ω6c, C14 : 0 2-OH, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) as the major cellular fatty acids. The predominant polar lipids were phospholipid, phosphatidylethanolamine and sphingoglycolipid. It took sym-homospermidine as the major polyamine. The DNA G+C content based on its draft genome sequence was 63.7 mol%. The polyphasic taxonomic analyses indicated that strain PRB40T represents a novel species of a novel genus within the family Sphingomonadaceae, for which the name Chakrabartia godavariana gen. nov., sp. nov. is proposed. The type strain of Chakrabartia godavariana is PRB40T (=MCC 3406T=GDMCC 1.1197T=KCTC 52678T=LMG 29985T).
Arch Biochem Biophys. 2019 May 31. pii: S0003-9861(19)30267-X. [Epub ahead of print]
Disturbance of I1-imidazoline receptor signal transduction in cardiomyocytes of Spontaneously Hypertensive Rats.
Maltsev AV
Evdokimovskii EV
Kokoz YM
Imidazoline receptor of the first type (I1R) in addition to the established inhibition of sympathetic neurons may mediate the direct control of myocellular functions. Earlier, we reveal that I1-mediated signaling in the normotensive rat cardiomyocytes suppresses the nitric oxide production by endothelial NO synthase, impairs sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) activity, and elevates intracellular calcium in the cytosol. Also, I1-agonists counteract β-adrenoceptor stimulation effects in respect to voltage-gated calcium currents. This study ascertains the I1R signal transduction in the normotensive Wistar and SHR cardiomyocytes. Reduction of Ca2+-currents by rilmenidine, a specific agonist of I1R, ensued from the phosphatidylcholine-specific phospholipase C-mediated activation of protein kinase C. There is a stimulation of serine/threonine phosphatase activity. In SHR cardiomyocytes, both the rilmenidine, and putative endogenous ligand, agmatine, almost twofold less effectively reduced L-type of Ca2+-currents. Average mRNA level of Nischarin, established functional component of I1R, is slightly decreased in SHR, whereas the protein levels are not significantly differed from normotensive rats. Disturbance of I1R signal transduction in SHR may aggravate the development of this cardiovascular pathology.
Adrenergic stress; Agmatine; L-type Ca(2+)-channel; PKC; Rilmenidine


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I got an early start to the day. When I head to the salon it's an all-day affair. My goals are in place and I'm going to work hard to make myself healthy and proud again. I've had a change of heart and mind recently and I'm looking at life and all my challenges in a… Read the Post »
Day In The Life Vlogs
Tornados, Peppers, Fruit, and Processing Tomatoes
Tornados, Peppers, Fruit, and Processing Tomatoes. It was an office workday and Greg was home working on the garden and picking food to be processed later in the day. It was my first day back at the office and we had a tornado come through. If you follow my Youtube channel you may have seen the… Read the Post »
Cooking Out and Getting Ready for the Week
Cooking Out and Getting Ready for the Week. The lettuce in the raised garden is coming in and today was first picking. I could not think of a better day to start picking than today. Greg made burgers on the grill so they will go on the burgers from this point until they are gone. I'm… Read the Post »
99lb Loss and Transformation Pictures
99lb Loss and Transformation Pictures Today I wanted to share where my weigh-loss journey started and why I did what I did. The journey was not always easy and it took a few years for me to finally get serious about my goals. I wanted to share my before photo of when I was 209lbs to… Read the Post »
Starting a Tree Garden
Starting a Tree Garden. Just a typical Saturday around the Rambles household. The weekend means it's time to work in the yard and add fruit trees. Our main goal was to get all the trees in the yard so they can get lots of sun. We spent some time to map out where we want to… Read the Post »
Dating Gone Bad
Dating Gone Bad. It was a typical Saturday of working in the yard to get ready for the growing season. I love that Spring is here and we can add to the garden and yard. One of the most exciting things about this house is that we grow our own food. It would be better if… Read the Post »
Cuddling with Baby Goats
Greg and I went to a local family farm to play with the goats. We found a place on Facebook that mentioned they offer time slots to cuddle with the baby goats. I figured Greg was all for it because he would love to talk me into moving to a larger piece of property. Goats are… Read the Post »
Learning to Drive a Stick Shift 😱
Learning to Drive a Stick Shift 😱. You will be happy to know that I've been taking my blood pressure medicine as I should. It's not always easy to remember but I'm doing better with each day. Greg made us a spicy breakfast this morning. We plan on speeding up my metabolism one way or another…. Read the Post »
So Many Fruit Trees
A beautiful Saturday morning and I spent it at the office working on my real estate class. It's a lot to go over and I feel like I have information overload. Look at the new picture we have for the office. It says 'one kind word can change someone's day'. I love the saying and it's… Read the Post »
Annoying Wife
We took Digger for his normal walk around the yard to check on the flowers and garden activity going on. He likes to be shown all the new growth and to talk time to smell the flowers. Spring is really showing up in so many ways now. Picking rocks out of the garden is starting to… Read the Post »


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Internet Service Provider Pelham Centre unlimited ISP. Pelham Centre unlimited Cable Internet & DSL internet (if available ). Pelham Centre ISP providing Excellent prices. Pelham Centre unlimited internet plans offered. Wonderful support. No long term commitments. Unlimited Internet Service Provider. WRS Web Solutions Inc. is an Internet Service provider (ISP).
Other packages available by request.
We offer 2 types of home internet to places like Pelham Centre. The first is over cable company TV network (which WRS Web Solutions Inc. pays for the use of, not you ). The second is over copper Bell phone lines in areas which are DSL (Digital Subscriber Line) capable, & one would require to have a live Bell phone line or order a 'dry loop' from our company. A 'dry loop' is a telephone line that is solely used for data. Cable internet packages are usually the better way to go. If you ask our team to Check My Address For Internet Availability our company will check it for cable internet & get back to you by means of email the same or next day. Should cable internet not available then we will in addition check it for DSL. DSL internet. Not all Bell telephone lines is able to get DSL internet. Should a potential customer has questions, one should ask them in advance of making an order. These orders have to be paid for when they are made as our company does not accept orders which have not been paid. Modem purchase or rental from us as part of placing the home internet order is usually required. Our firm accepts Interac e-Transfer, credit cards and PayPal account as ways of paying. One should note that our company uses PayPal as our credit card processing, that said one does not need to open a PayPal account. Once you make your residential internet order you would be displayed an Invoice that has two yellow buttons in it. One says 'PayPal Subscribe' and the second says 'PayPal Checkout'. If a customer wishes to create a monthly automatic subscription with your credit card or using a PayPal account please use the 'PayPal Subscribe' button, or to just pay the Invoice select the 'PayPal Checkout button option.
Unlimited Internet Service Providers (ISP) Pelham Centre. WRS Web Solutions Inc. simply the best choice when looking for an unlimited Internet Service Provider. Get internet offers. Very good internet plan price. Internet Service Providers (ISP) Pelham Centre. Compare Pelham Centre unlimited Cable and DSL internet service provider Pelham Centre, Ontario cable or DSL internet packages. These Pelham Centre high speed internet packages. Pelham Centre Cable and DSL Internet Service Provider (ISP) have differences when it comes to internet plans. Compare internet service providers who sell internet Pelham Centre. For both home or business internet for Pelham Centre residents compare our Pelham Centre high speed internet service providers rates with our company Pelham Centre high speed internet service provider, & best ISP. WRS Web Solutions can provide low cost high speed internet service provider to just about every house in Pelham Centre that is serviceable with cable TV, and internet capable DSL telephone lines. Pelham Centre business cable internet is often available at Pelham Centre high speed internet home prices (or close to them ). High speed internet service provider delivering unlimited plans.
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Cheap internet Pelham Centre at sweet prices. Switch internet from your current ISP. We want to be your Internet Service Provider (ISP)! Our packages may be cheaper than your present Internet Service Provider. Compare us to your current Internet Service Provider. Then choose the Internet Service Provider which is cheapest.
Best Internet Service Provider Pelham Centre. Best internet costs for Pelham Centre homes. ISP Pelham Centre. Pelham Centre internet packages are delivered utilizing rented Cogeco Pelham Centre cable TV lines by WRS Web Solutions Inc. Compare Internet Service Provider prices and consider the switch from Cogeco Pelham Centre cable internet to WRS Web Solutions Inc. cable internet. Modem purchase & complete order payment is usually necessary when placing the order. Our company is not a Pelham Centre wireless internet service provider. Pelham Centre DSL internet might be available if Pelham Centre cable internet isn't. Packages do not come with Pelham Centre Home telephone or Pelham Centre cable TV services. Look for the best Internet Service Provider!
As an Internet Service Provider, we do not offer wireless internet, satellite internet, 'point to point' internet, towers or wireless hubs. Our firm is not in a position to 'wire' unserviced parts of the Province of Ontario, utilising either the cable TV or telephone networks, or build DSL capability where Bell phone lines do not already have it as we are using Bell's DSL infrastructure. Thus if you can presently get Bell DSL then you can get our DSL (up to the speeds we are allowed to offer ). WRS Web Solutions Inc. does not sell fibe TV packages, or cable TV plans, despite that the bulk of our clients use Netflix in place cable TV.
Internet Providers Pelham Centre. Compare cheap internet plans for Pelham Centre. Like various Pelham Centre internet service providers, our Pelham Centre high speed internet plan speeds below are in Mbps, & are 'up to'. 'Fee' refers to an Installation or Activation or Transfer fee, & may be referred to as a 'setup fee' in some parts of the company's website. Rates are monthly, well priced unlimited internet service provider packages Pelham Centre. Now that's a great internet Pelham Centre price! Plans are normally made available through cable internet Pelham Centre lines, as opposed to Pelham Centre telephone lines (unless no cable lines are available at that address or area in which case we may have DSL internet plans), & as such are frequently not available in non-urban areas unless cable is available there (or unless DSL internet is available). Curious about the price of internet Pelham Centre? This is cheap home cable internet marketed by the best internet providers Pelham Centre. Locate a cable internet Pelham Centre providers list and compare high speed internet providers or consider our company's internet deals Pelham Centre. Pick WRS Web Solutions Inc. as your Internet Service Provider.
Before checking your Postal Code, & particularly in advance of asking WRS Web Solutions Inc. to lookup to see which DSL and / or cable internet plans are deliverable at your address, please be aware that in Ontario we use Cogeco, Rogers & Shaw Pelham Centre Cable TV networks to market our cable high speed internet services, (we pay a price to utilize those cable TV lines therefore if you have previously asked to order cable high speed internet from both of those internet service providers and they could not deliver cable high speed internet, then our team would be unable to supply you with cable high speed internet either. On the plus side if you currently have (or ever had, or anyone else at that address ever had) cable internet services with the cable company at your current address you can have our cable internet services no need to contact us or look anything up.
In addition we do also offer DSL high speed internet over the Bell phone lines (in the very same areas where Bell Pelham Centre is capable of providing DSL), but if you already contacted Bell & could not obtain phone line based internet services from them, then we would be reply to you the exact same answer. If you do not have an active telephone line with Bell, when you order DSL internet from us, then you will require a 'Dry Loop' that is an added monthly cost of around $15.95 in addition you must order that from us as part of the on-line order process as a 'add on' option on the order.
In addition please be aware that this Postal Code database is still rather incomplete (especially for smaller places) because the large firms which own the networks are unwilling to share their Postal Code location data with us because it provides them a competitive advantage over us knowing where the lines are & are not, & we must build up our own database search by search one at a time, gradually. Therefore, if your Postal Code is not in the Postal Code that does not mean we cannot service the address. Thanks in advance for your understanding. ☺
Unlike many Internet Service Providers, we do not take orders by phone, they must be done online using the website. We try to avoid taking inquires by phone, rather we prefer to be contacted through the Sales or Support contact forms, that way we may research a suitable written response. For Pelham Centre website hosting, questions please use the Support form.
To view our list of approved modems in a separate window, please click here
The above Internet modem Pelham Centre listing of Pelham Centre Ontario internet high speed suitable modems (for use with our well priced internet Pelham Centre services) is up to date from 15 Oct 2018, & is subject to change at any time without notice.
Find internet in Pelham Centre. Compare internet plans for internet providers in my area. Our company can check on your behalf! Check My Address For Internet Availability
Internet availability in my area Pelham Centre, Ontario. Internet Service Providers. Ask us if Pelham Centre Cable internet & DSL internet available in my area. Research My Address For Internet Availability
As an Internet Service Provider, we sell a SmartRG SR505N wireless modem at $99.95 and $12.95 postage. You would have the option to buy a modem when placing an order for Pelham Centre phone internet with our company - the top internet service provider Pelham Centre.
When it comes to deciding to go with a DSL high speed internet service provider in Pelham Centre, for your Pelham Centre residence, our firm feels that we offer the the best value month to month Pelham Centre internet packages no contract. Normally one could even switch internet service providers to WRS Web Solutions from some other internet service providers in Pelham Centre. WRS Web Solutions Inc. is an alternative Pelham Centre internet to the various Pelham Centre internet service providers. We offer internet in Pelham Centre. As an internet service provider, our firm has several of Pelham Centre's best internet packages.
Should you buy a Pelham Centre DSL internet plan rather than a cable internet package a DSL Modem is required to receive DSL internet service in Pelham Centre. Should you be have a rental modem from Bell you would need to plan a date to bring it back it on. You must provide an approved modem (or rent or buy a suitable from us and we will send it to you) or that suitable modem won't function with our Pelham Centre internet services). If you own your own suitable modem you need to share with us the information we need (make / model, serial number, plus the 'macid' or 'machine ID') when you make that Pelham Centre high speed internet order online with us. We retail both non-wireless modems and wireless modems, please be sure to buy the right kind! If you order a modem from our company, a delivery fee applies when you buy it (shown in the ordering system as a 'setup fee' but it is really for 'shipping' and not for any sort of 'setup').
Pelham Centre DSL Internet Service Provider if DSL is available. It is important to be conscious of the fact that WRS Web Solutions Inc. doesn't have permission to access Bell FTTN lines. Be sure to ask us to check the address for serviceability prior to placing an order. Paying is necessary when the order is placed. Billing begins from day of order. If you do not have an active Bell phone at your Pelham Centre home, we will have to bill for a 'dry loop' (a telephone number & line which is only for internet use and cannot be used to for phone calls). Our firm also retails an optional Telephone line noise filter for a small one time cost. Static IPs are offered for people who wish them, but most people do not require them. Plans do not include TV channels, home phone, or security services.
WRS Rateband A, B, C, D, E, F - DSL Dry Loop $15.99
WRS Band G - DSL Dry Loop $25.99
ALL ADSL2+ compliant ADSL modems may be used on the Bell lines in Pelham Centre with ADSL speed tiers (6/0.8, 7/1, 10/1, 15/1), but you needs to own that modem (or buy or rent one from us). If the current modem was belongs to Bell, you need to return it. It can't be used with our services. Regarding ADSL, it would be a SmartRG 501, which is only a modem (no built in router) that our website does offer. The SmartRG SR515AC modem is able to do both ADSL and VDSL may be used in the event that you desire a modem router combo on an ADSL connection. As for the SmartRG SR515AC Wireless Modem, we presently retail this modem at roughly $129.99 plus shipping of $12.95
A Modem is required to have internet. We presently sells modems (& can vary with plan speed) for Pelham Centre internet users. You would have the opportunity to buy or rent one :as part of placing the order for Pelham Centre DSL internet. You must provide a suitable modem (or purchase or rent an approved modem from us and WRS Web Solutions will ship the modem to your address) . If you have a suitable modem you need to provide our team the particulars we need (make / model, serial number, as well as the 'macid' or 'machine ID') at the time you make your Pelham Centre internet package order online with us.
Like most Pelham Centre DSL internet service providers, the Pelham Centre DSL internet plan speeds advertised here are maximum speeds under perfect circumstances (as they say 'up to'). Results can be different because of many situations, most of them are beyond our control, despite then fact that WRS Web Solutions Inc. always strives to provide the best internet services in Pelham Centre. For supplemental info regarding a variety of Pelham Centre internet packages topics check out the Knowledgebase and the FAQs page of our website.
The Pelham Centre phone number you supply for your customer account at the sign-up is the same phone number we will give to the Pelham Centre DSL Internet Installer, who could call to confirm the appointment If nobody answers the call the Installers will assume that no one is there and not come Please answer the call. If your Pelham Centre residence is already wired for DSL, the Ontario Installer might not need to enter your Pelham Centre home, so please connect the modem with the high speed internet DSL & the computer to the modem (if not a wireless modem), then please try the internet connection on the activation date (and time of day) and see if it is running or not. If it is connected that is good news. If you did not get a call and the Installer did not come and it is not live, please tell us by Help Desk Ticket.
Please note that watching Netflix and various steaming and downloading services may very rapidly use a lot of that Pelham Centre internet usage & unless one has an 'unlimited' internet package this could very quickly get costly in internet fees. We are not able to provide any pop up advisory when a customer exceeds the usage in the Pelham Centre high speed internet plan, & no easy way for you to look it up either, you usually have to open a Help Desk ticket to ask us (& it might take us a day to respond by email, or you could wait for your internet overage bill to come by email, so please be conscious of how much use you make of such high speed internet streaming & download services! Please realize that often we do not get the overage number figures until the following month, so you could receive an Invoice for overage expenses for the prior calendar month.
The above Pelham Centre packages prices, availability, overage rates, packages, etc, and other info, may change without notice. Not all plans are available at all Ontario addresses. Modem prices and availability are subject to change. Sales taxes apply. Terms & Conditions, Traffic Management Policy and Privacy Statement apply. The Pelham Centre internet services are a month to month and pay in advance service. Customers are invoiced through e-mail on the first day of the month for that month. Overage fees could be a month later in showing on your account. Billing begins from the date of order not date of installation/activation. The preferred way to Contact WRS Web solutions Inc. is through the Help Desk. WRS Web Solutions Inc. is a Canadian company, residing in the Province of Ontario. It is a separate company, from Bell & the cable companies. It pays a fee when makes us of the phone and cable internet networks.
Switch internet service providers in Pelham Centre. Thinking of switching internet service providers in Pelham Centre to our cheap Pelham Centre high speed phone internet plans Review our cheap Pelham Centre high speed phone internet packages? These value priced Pelham Centre high speed internet plan options are yours to pick from. Curious if WRS Web Solutions Inc. can offer low cost internet package services at your Pelham Centre residential address? If a person be in a contract for Pelham Centre internet they might not be able to switch from internet service provider to our Pelham Centre internet plans until that contract has ended. As opposed to some companies our high speed internet plans do not have contracts, and no cancellation fees either! Switch Internet Service Provider!
Cheap Pelham Centre DSL Internet rates from our company your Pelham Centre high speed internet service provider, cover not only Pelham Centre, but various additional regions of the Province. The main advantage of our best Pelham Centre high speed internet plans over other high-speed internet (or other companies) is in the company's thoughts and opinion the price, (i.e. internet service Pelham Centre cheap) but you have to compare best cheap Pelham Centre high speed internet packages and prices and make a decision on your own. The second advantage is the fact we do not need term contracts for our cheap Pelham Centre high speed internet prices. We think that creates a good high speed internet providers Pelham Centre comparison. Cheap internet in Pelham Centre, & many of the very best internet rates in Pelham Centre.
Need high speed internet prices for Pelham Centre. Looking for internet alternatives Pelham Centre? Not having a whole lot of success? Need cheaper internet? Should you have previously attempted to get Pelham Centre broadband internet service from Cogeco and Bell, or another cable or DSL internet providers Pelham Centre, & they said they cannot offer service at your Pelham Centre address, then please do not try to get it from us, as we use the Bell phone system to offer our best cheap Pelham Centre high speed internet plans as well, and without their phone line to you Pelham Centre home, we aren't able to sell our best cheap Pelham Centre broadband DSL internet service either (much as we would like too). Same applies if you checked on the Bell site, whether it said that the telephone land line based form of Pelham Centre high speed internet was not available at your Pelham Centre Postal Code, address or phone number, then we will have the same problem. Thus, no point in asking our company if we can offer service, as we cannot. So please don't make such an order for internet.
Our Pelham Centre high speed internet plans are very competitive, even as we are among the best high speed internet service providers Pelham Centre. These best cheap Pelham Centre high speed internet plans are billed from 'date of order' not from date of installation or activation. Your 1st month's best cheap high speed internet package service will be prorated, and then billed by calendar month. If you decide at a later time to change your internet plan with us, a small change cost might apply so please pick the best Pelham Centre high speed internet plan for your requirements. You'll be invoiced by email, credit cards are not charged automatically except if one has a PayPal Automatic Payment agreement (subscription) in Pelham Centre, and also you must pay the Invoice online though the payment gateway provided. WRS Web Solutions Inc. accepts credit cards & PayPal accounts. A couple days after we accept your order we will contact you with your modem shipping particulars, & later the Installation/Activation day and time slot
As with the majority of the best cheap Pelham Centre high speed internet service providers (ISP), the Pelham Centre high speed internet plan wire internet speeds in the above list are maximum speeds under normal conditions ('Up to'), results may vary according to numerous conditions, almost all of those are beyond our control, even though we constantly strive to give the best internet services Pelham Centre. Internet speed Pelham Centre, for internet services Pelham Centre. We believe our speeds compare well against our rivals. To view additional info over a bunch of internet packages regarding Pelham Centre subject areas please refer to our Knowledgebase. Our DSL packages will not be any faster than the equivalent advertised speeds sold by Bell or Cogeco.
ISP Pelham Centre. WRS Web Solutions Inc. is your best ISP Pelham Centre high speed internet providers. WRS Web Solutions Inc. cares. WRS Web Solutions Inc. has excellent costs.
The Pelham Centre telephone number you provide on the account at time of ordering is the telephone number we might give to the Internet Installer, who will phone to confirm the appointment. If your Pelham Centre home is already wired for internet, the Pelham Centre Installer might not would like to enter your Pelham Centre home, thus please connect the modem to internet line, & the computer to the modem (if not a wireless modem), then please attempt the high speed internet connection on the activation date (& the appointment time slot) & check if it's operating or not. If it is operating that is wonderful news If you did not get a phone call, and the Pelham Centre Installer did not come, and the connection is not working after midnight on the installation date, please let us know us through the Help Desk Ticket.
Please be positive that you give a preferred Installation date once you place your order online with us, and please ensure that date is leastwise seven days in the future, because the third party Pelham Centre Installers are typically already booked one week out. Please also have the needed time of the day for example AM, PM, evening, etc.
We would ask that you take note that this is your responsibility when watching and downloading movies, TV series, and other content, utilizing your Pelham Centre high speed internet package to ensure that you doing so legally. When ever you do any such activity your Pelham Centre Internet protocol address is usually noticeable to such websites, and is kept by them (and maybe others) and might be shared by those sites to companies which search for folks from Pelham Centre who have illegally viewed copyrighted materials using their Pelham Centre internet plan. Such companies could then send us 'copyright infringement notices' as per the Copyright Act, for us to forward to you, and our firm has no legal option but to send them to you, therefore please be careful with what you stream or download (and from where), with your Pelham Centre high-speed internet plan as all usage is never 'anonymous' regardless of what others might assure a customer. An example is the use of Android TV boxes. Thanks in advance.
WRS Web Solutions Inc. is an internet service provider (ISP) that was incorporated in 2010. WRS Web Solutions Inc. is not solely an internet service provider. For instance in addition to being an internet service provider the company is also a domain name provider and a web host company, and it retails website related services. WRS Web Solutions Inc. is registered with the CRTC as an Internet Service Provider (ISP). Internet Service Provider related media enquirers are very welcome
If you like our internet packages and our customer service, please tell your friends, relatives, neighbors & coworkers. If you do not, please tell our folks and please allow our staff an opportunity to correct the situation. If you are not currently a customer, then please give us an opportunity at being your internet service provider.


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Japan has demanded that the US ground its V-22 Osprey aircraft deployed in Japan after one of the Marine Corps planes crashed off Australia.
The crashed Osprey was among the tilt-rotor aircraft deployed at U.S. Marine Corps Air Station Futenma in Japan's southern island prefecture of Okinawa, where local residents have expressed concern about the safety of the aircraft that have a history of fatal accidents, Kyodo news agency reported.
Japan plans to discuss with the US whether it would be appropriate for Ospreys to take part in joint training between the Japanese Ground Self-Defense Force and the U.S. Marines in Japan's island of Hokkaido, planned to be held from Thursday, Defense Minister Itsunori Onodera told reporters Sunday as reported by Kyodo news agency.
Onodera said his country would demand a "solid explanation" about the accident. He was speaking with reporters after an Osprey crashed off the coast of Shoalwater Bay near Rockhampton in Queensland state Saturday, leaving three U.S. Marines missing while 43 others were rescued.
Onodera said Japan urged Washington to disclose information about the accident, investigate what caused it and take measures to prevent such an accident from happening again.
The Osprey aircraft, which can take off and land like helicopters but cruise like fixed-wing airplanes, have one of the worst safety records among military transport aircraft in the world. Built by Bell-Boeing, the unique planes are intended to take marines or special forces quickly to the scene of action. They can land and take off from aircraft carriers.
In December last year, a U.S. Marine MV-22 Osprey crash-landed in waters off the main island of Okinawa. In May 2015, a U.S. Osprey made a hard-landing in Hawaii, resulting in the deaths of two Marines.
Two B-1B bombers under the command of US Pacific Air Forces have joined their counterparts from the Republic of Korea and Japanese air forces in sequenced bilateral missions in response to North Korea's ballistic missile and nuclear programs. This mission is in direct response to North Korea's escalatory launch of intercontinental ballistic missiles on July 3 and July 28
Japanese Defense Minister Tomomi Inada has tendered resignation taking responsibility over an alleged cover-up involving daily activity logs of Japanese troops serving as UN peacekeepers in South Sudan. Foreign Minister Fumio Kishida will take over as interim defense minister until new chief is appointed, Prime Minister Shinzo Abe was quoted as saying by
The Indian Ministry of Defense has issued Request for Information to Mitsubishi Heavy Industries and Kawasaki Heavy Industries of Japan, Naval Group (formerly DCNS) of France, Navantia of Spain, Rosoboronexport Rubin Design Bureau of Russia, Saab of Sweden and ThyssenKrupp Marine Systems of Germany to build its new line of six conventional submarines at an estimated cost of $7.7 billion
Japan's Air Self-Defense Force will equip its F-2 fighters fleet with a new air-to-ship missile that travels three times faster than the type 93, from next fiscal year. The government will allocate huge amount in a budgetary request for fiscal 2018 to mass produce the missile, which is currently under development
Chinas Peoples Liberation Army Air Force (PLAAF) flew six Xian H-6K long-range bombers through the strategically important Miyako Strait Thursday. The Miyako Strait is located between the Japanese islands of Miyako and Okinawa
Japans Air Self-Defense Force will deploy a Patriot Advanced Capability 3 (PAC-3) surface-to-air guided missile unit for a joint training exercise with US troops next month at the hub of the two allies' Yokota Air Base. The exercise, which could be held as early as August, is to test the two forces capabilities against increasing military provocations by North Korea in the form of ballistic missile launches, local media reports
US Marines commander of Japan-based Osprey squadron has been sacked 'due to loss of trust following a series of accidents involving the rotorcraft in the past few months. Bryan Swenson was relieved of his duties last week, "due to loss of trust and confidence in his ability to lead the command," III Marine Expeditionary Force was quoted as saying in a statement to AFP on Friday
Two MV-22 Osprey aircraft made emergency landings at Ishigaki airport in southern Japan today, the administrative office of Ishigaki city told Mainichi Japan. The U
Bell-Boeing is being awarded $40 million modification contract for the procurement of long-lead components associated with the manufacture and delivery of four fiscal year 2018 Lot 22 MV-22 Osprey tilt-rotor aircraft for the government of Japan. Work is expected to be completed in September 2018, US department of Defense said in a statement Thursday
Japan has launched third of its new quasi-zenith satellites on Saturday to improve the global positioning system (GPS) navigation services. The launched third of its new quasi-zenith satellites is one of a constellation being put into orbit to improve the accuracy of available global positioning data,
Up to six MV-22 Ospreys with the US Marine Corps will join a US-Japan military exercise from Friday, Japanese Defense Minister Itsunori Onodera told reporters in Tokyo Tuesday settling doubts about the fitness of the aircraft which had crashed off Australia last week. "Japan also believes there is important significance in the Osprey's participation in the exercise
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Table of Contents :
Top Suggestions 4 Printable Worksheet For Grade K 5 :
4 Printable Worksheet For Grade K 5 Whether your child is struggling to grasp the rules of two digit multiplication doesn t quite get the nuances of suffixes or simply wants to learn more about important figures in african american Is at the front of the classroom repeating the steps onto her own worksheet projected onto a large screen for those students Scholastic teachables contains tens of thousands of searchable and printable educational materials the content is searchable by topic theme grade level and project and includes a variety of.
4 Printable Worksheet For Grade K 5 Since the early s colbert says she has reached some 5 the 4 year olds up to the tallest one for the 10 year olds each has a notebook open alongside the learning guide at one point the A grandmother from massachusetts told this story my 5 year old of drill and worksheets and reduces other vital areas of learning such as math science social studies art music and creative Government funding can help o keefe amp lewis new report shows 4 grade level and irrelevant to their lives we routinely.
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Duke nell k 3 6 minutes per day the scarcity of informational texts in first grade reading research quarterly 35 2 202 224 fox mem what next in the read aloud battle win or One of the sites teachers are using is share my lesson which offers more than 256 000 classroom resources for grades k 12 ranging from in a national teachers union representing 1 5 million We ve recently released more anchor pages in grades k 2 that help teachers take a targeted approach the two syllable double consonant flashcards is a printable resource that allows teachers to.
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4 Printable Worksheet For Grade K 5. The worksheet is an assortment of 4 intriguing pursuits that will enhance your kid's knowledge and abilities. The worksheets are offered in developmentally appropriate versions for kids of different ages. Adding and subtracting integers worksheets in many ranges including a number of choices for parentheses use.
You can begin with the uppercase cursives and after that move forward with the lowercase cursives. Handwriting for kids will also be rather simple to develop in such a fashion. If you're an adult and wish to increase your handwriting, it can be accomplished. As a result, in the event that you really wish to enhance handwriting of your kid, hurry to explore the advantages of an intelligent learning tool now!
Consider how you wish to compose your private faith statement. Sometimes letters have to be adjusted to fit in a particular space. When a letter does not have any verticals like a capital A or V, the very first diagonal stroke is regarded as the stem. The connected and slanted letters will be quite simple to form once the many shapes re learnt well. Even something as easy as guessing the beginning letter of long words can assist your child improve his phonics abilities. 4 Printable Worksheet For Grade K 5.
There isn't anything like a superb story, and nothing like being the person who started a renowned urban legend. Deciding upon the ideal approach route Cursive writing is basically joined-up handwriting. Practice reading by yourself as often as possible.
Research urban legends to obtain a concept of what's out there prior to making a new one. You are still not sure the radicals have the proper idea. Naturally, you won't use the majority of your ideas. If you've got an idea for a tool please inform us. That means you can begin right where you are no matter how little you might feel you've got to give. You are also quite suspicious of any revolutionary shift. In earlier times you've stated that the move of independence may be too early.
Each lesson in handwriting should start on a fresh new page, so the little one becomes enough room to practice. Every handwriting lesson should begin with the alphabets. Handwriting learning is just one of the most important learning needs of a kid. Learning how to read isn't just challenging, but fun too.
The use of grids The use of grids is vital in earning your child learn to Improve handwriting. Also, bear in mind that maybe your very first try at brainstorming may not bring anything relevant, but don't stop trying. Once you are able to work, you might be surprised how much you get done. Take into consideration how you feel about yourself. Getting able to modify the tracking helps fit more letters in a little space or spread out letters if they're too tight. Perhaps you must enlist the aid of another man to encourage or help you keep focused.
4 Printable Worksheet For Grade K 5. Try to remember, you always have to care for your child with amazing care, compassion and affection to be able to help him learn. You may also ask your kid's teacher for extra worksheets. Your son or daughter is not going to just learn a different sort of font but in addition learn how to write elegantly because cursive writing is quite beautiful to check out. As a result, if a kid is already suffering from ADHD his handwriting will definitely be affected. Accordingly, to be able to accomplish this, if children are taught to form different shapes in a suitable fashion, it is going to enable them to compose the letters in a really smooth and easy method. Although it can be cute every time a youngster says he runned on the playground, students want to understand how to use past tense so as to speak and write correctly. Let say, you would like to boost your son's or daughter's handwriting, it is but obvious that you want to give your son or daughter plenty of practice, as they say, practice makes perfect.
Without phonics skills, it's almost impossible, especially for kids, to learn how to read new words. Techniques to Handle Attention Issues It is extremely essential that should you discover your kid is inattentive to his learning especially when it has to do with reading and writing issues you must begin working on various ways and to improve it. Use a student's name in every sentence so there's a single sentence for each kid. Because he or she learns at his own rate, there is some variability in the age when a child is ready to learn to read. Teaching your kid to form the alphabets is quite a complicated practice.
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text: Chatty Kids Organisational Profile Chatty Kids is developing the world's first online, interactive, graded library of English picture and chapter books, with read-along videos. Chatty Kids is building strategic partnerships in the Chinese education market. In China, English is compulsory for primary school students and valued by parents as the path to international education and high-level employment. However, access to local or remote tutors who are native English speakers, and to high quality educational resources, is limited and expensive. On any internet-connected device, the user selects an e-book from the Chatty Kids library and follows along as a native English speaker reads the text in a video window. The child can record a video of him/herself reading aloud, to share with other children for peer-to-peer learning, and for assessment and feedback by educators and parents. Globally, the Chatty Kids platform will be the most cost-effective way for schools or parents to access a sufficient number of engaging, graded books, to help children develop English literacy and fluency. Services Provided gemaker wrote the successful Chatty Kids application for a $500,000 Accelerating Commercialisation grant from the Australian Government. I was determined to submit the best grant application possible, for the best chance of success, so I needed professional assistance. There is much more to it than simply filling out a form; there is special terminology and a particular writing style required, which gemaker knows and applies well. The grant assessors rejected two Chatty Kids applications prepared by other writers. With gemaker's help, my application was successfully completed to their exacting standards. The gemaker team pulled out all the stops and got the work done in a very tight time frame. They handled the pressure well and were pleasant to work with. I would certainly recommend them. Ken Taggart Founder and Director, Chatty Kids Related Success Stories The Advance Queensland IndustryTech Fund (ITF) Solve Innovation Challenges Falling Walls Lab Australia The AustCyber Projects Fund INNOVATION XCHANGE INAUGURAL OPTUS INNOVATION CHALLENGE XPRIZE Thinkable NSW Pitch to Pilot Program Queensland Small Business Innovation Research Program (SBIR) Small Business Innovation Research for Defence (SBIRD) Business Research and Innovation Initiative (BRII) (DIIS) The IOT/WT Innovation World Cup Horizon Prize – Cleanest Engine of the Future The Humanitarian Innovation Hackathon Legal Access Challenge Galileo Masters Idea of the Year Galileo Masters Startup of the Year 2020 Millennium Technology Prize Creating Hope in Conflict: A Humanitarian Grand Challenge Cyber Security Challenge Australia 2019 Transport Pitchfest Active Australia Innovation Challenge Fast Track: Improve the Site Access Experience WYSIWYG 3D UNSW University of Sydney UTS Data61 UOW ANSTO Integra Law Life Cell Marine Safety Monash University Innovation ANU Smart Services CRC (iSee) Stormseal Industries Pty Ltd Alkane Resources University of Wollongong CRC for Low Carbon Living University of Technology Sydney Katie Cowling – Digital Marketing Manager Swinburne University of Technology Chatty Kids Data61 CSIRO IP Australia Beasley Intercultural Pty Ltd SynFlyt Rebecca Colless – Communications Specialist Dr Ellen Gregory – Communications Specialist Jessica Simpson – Operations & Resourcing Manager Dr Michael Chapman – Technology Manager Knowledge Commercialisation Australasia (KCA) Dr Anna Figueiredo, Commercialisation Advisor Paula Moss – Technology and Market Assessor Cruiser Interactive CAST CRC Yvette Hlavaty – Technology and Market Assessor BioGill ANU's Advanced Instrumentation Technology Centre VPAC INNOVATIONS Pty Ltd ANSTO Minerals Dr Ken Crane Dr Julie Wheway Natalie Chapman James Dalton – Commercialisation Manager Beasley Intercultural SynFlyt


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This week saw the launch of our much-anticipated behind-the-scenes 'Champions' DVD, and the response has been amazing. The club would like to thank everyone who has purchased a copy, as supporters have made the most of the opportunity to relive last season's unforgettable title triumph. Exiled supporters are reminded that we are also welcoming orders
Torquay United are inviting Season Ticket holders to join us in celebrating last season's unforgettable title triumph by living the dream and lifting the National League South Trophy! Our first event will take place prior to the Aldershot home match on Saturday, 24th August, and will be exclusive to Season Ticket Holders only. This will
Excitement is growing at Plainmoor ahead of our forthcoming televised clash against Aldershot Town this Saturday, 24th August (5.20pm KO). With BT Sport covering the game live, it promises to be an exciting occasion for everybody involved with the club, and we will be opening early to allow supporters to savour this special matchday experience.
United head to London on Bank Holiday Monday, as Gary Johnson's side take on Barnet at The Hive. Tickets for the match are available directly from Barnet FC via the link here, with Gulls fans being situated in Stand Sixty-Six Blocks A & B. Please note that our allocation is all-seater. Ticket prices are as
Striker Manny Duku notched a hat-trick as United's second-string recorded an impressive 7-0 win over Bristol Rovers' Development Squad at Lodge Road this afternoon. Torquay took the lead in the ninth minute, as Trialist A side-footed home from the edge of the area. A spell of pressure from the home side followed, before Duku opened
The Club was saddened to learn of the recent passing of former Torquay United favourite Doug Clarke at the age of 85. Clarke, who began his league career with Bury, enjoyed a remarkable ten year spell with Hull City prior to joining Frank O'Farrell's side in the summer of 1965. During his first season with


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Accession Number : ADA603304
Title : Bearing Assembly With Carbonate Resistant Anti-Seizing Properties
Descriptive Note : Patent application, Filed 28 Jan 2014
Corporate Author : NAVAL UNDERSEA WARFARE CENTER DIV NEWPORT RI TECHNOLOGY PARTNERSHIP ENTERPRISE OFFICE
Personal Author(s) : Wiedenheft, John H
Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a603304.pdf
Report Date : 28 Jan 2014
Pagination or Media Count : 15
Abstract : A bearing assembly including a load-carrying shaft, an electrically non-conductive sleeve fitted over the shaft, and a surrogate shaft surface made from a corrosion resistant metal surrounding the non-conductive sleeve. The bearing assembly electrically isolates the load-carrying shaft from an underwater structure. This isolation prevents carbonates from building up in the void space between the metallic surrogate shaft surface and a bearing. A rotor or slider subassembly may be included in the bearing assembly. A bearing may be affixed as part of the rotor or slider assembly located between the slider and the surrogate shaft surface.
Descriptors : *SHAFTS(MACHINE ELEMENTS) , ASSEMBLY , BEARINGS , CARBONATES , CORROSION RESISTANCE , METALS , PATENT APPLICATIONS
Subject Categories : Machinery and Tools
Distribution Statement : APPROVED FOR PUBLIC RELEASE