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Investment Strategies
An active approach to equity investing that's designed to capitalize on global market trends. Tailored to align with your risk and goals.
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Environmental, social and governance (ESG) integrated approach that seeks to capture global market trends while rewarding funds that aim to make a positive impact. Customized to support your values and goals.
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Award-winning flexible approach to fixed income that targets opportunities for gains in the bond market. Seeks to mitigate risk by limiting exposure to more volatile areas of the bond market and through active management.
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Let's set up a time to talk and see if we're a good fit.


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It wasn't all that long ago that Matt Cutts, the head of Search spam at Google, stated his recommendation that you should nofollow links in widgets since the widget space has had an abundance of link spam abuse. In fact, it was a mere five months ago.
But, in that short of time, something has changed a little since then. A small, yet significant update to the Google Webmaster Guidelines link schemes page has been made. In the update, the page now specifies the exact type of links that will be allowed within widgets. This is a change from before when it stated that it could be any link.
Previously the guideline read:
Links embedded in widgets that are distributed across various sites.
Today the guideline reads:
Keyword-rich, hidden or low-quality links embedded in widgets that are distributed across various sites.
This may be a small change to their stance on putting links in widgets, but it's a positive step. At least now they're being specific to spammy keyword packed, hidden or low-quality links. So now it's ok to put a link in your widgets, so long as everything about it is cool.

subset: commoncrawl


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Whether it's in their acclaimed Méthode Cap Classique (MCC) sparkling wines, or the iconic blend of Chardonnay and Pinot Noir, the von Arnim family of Franschhoek winery Haute Cabrière has long been passionate about the noble cultivars of Chardonnay and Pinot Noir.
In the 35 years since founder Achim von Arnim planted his first vines on Farm Cabrière in the heart of the Franschhoek Valley, the cellar has never released a stand-alone Chardonnay – until now.
Two years after taking over production at Haute Cabrière, cellar master Takuan von Arnim has taken the pioneering decision to release the winery's maiden Chardonnay Réserve from the 2016 vintage.
"Haute Cabrière has always been a cellar focused on Pinot Noir and Chardonnay. They're the varietals my father has always loved, and the launch of our Chardonnay Réserve is perfect in showing respect for who has worked so hard for so many years," says Takuan.
The new release is an impressive addition to the cellar's sought-after Réserve Range, joining the respected Pinot Noir Réserve and award-winning Blanc de Blancs MCC as the purest expression of Haute Cabrière's unique terroir and winemaking philosophy.
Adding a new wine to Haute Cabrière's focused portfolio was a natural progression for cellar master, Takuan von Arnim. Cabriere is certainly no stranger to producing superb barrel-aged Chardonnay which forms an integral part of the winery's acclaimed Blanc de Blancs Méthode Cap Classique – a 4½-star recipient of the 2017 Platter's South African Wine Guide.
"You can't rush into these things. Fortunately our experience with the cultivar and excellent working relationship with the coopers led us to believe we could do it properly," says von Arnim.
All the fruit for the Chardonnay Réserve 2016 was sourced from Haute Cabrière's own vineyards, and harvested by hand. After whole-berry pressing, the wine was fermented in oak – 60 percent new, 40 percent second-fill – and allowed to rest on the lees until 30 percent malolactic fermentation was complete. The wine was then 'rough racked' into stainless steel tanks before being returned to barrel for a further eight months.
The result is certainly impressive: a Chardonnay in the classic Burgundian tradition. Defined by elegance and vitality, the oak supports, not dominates, the primary fruit characters. Expect a nose of vibrant citrus, melon and white pear, backed by a generous mid-palate underpinned by elegant oak tannins.
"It's more European than New World in style," adds von Arnim, who says the well-judged use of oak was key to ensuring a wine retaining plenty of freshness. A Chardonnay you can enjoy with oysters!"
While Takuan is thrilled that years of careful planning have come to fruition in the bottle, what does the founder of Haute Cabrière, Achim von Arnim, make of the cellar's maiden Chardonnay Réserve?
"He's extremely proud that we have added our signature to the Haute Cabrière Wine portfolio," smiles Takuan.
Only 1000 bottles of the Chardonnay Réserve have been produced in the maiden 2016 vintage. The wine will be available from the cellar door and restaurant at R190 per bottle from the end of September 2017.
Haute Cabrière's Restaurant and Tasting Room are situated on Franschhoek Pass (Lambrechts Road), Franschhoek. Tasting Room Hours are: Mon – Fri: 09:00 to 17:00 – Saturdays and Public Holidays: 10:00 to 17:00 – Sunday: 11:00 to 16:00 Contact details for reservations and enquiries: Email firstname.lastname@example.com or call 021 876 8500.


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Fans need not worry about @realcurtisaxel being successful. He has heart & desire plus DNA. Lets not 4get @heymanhustle. Don't over analyze.
— Jim Ross (@JRsBBQ) May 25, 2013
That sounds completely reasonable upon first glance. His response to the next Tweet kind of baffles me and I think this is what speaks to WWE's flawed logic.
"@jeffdimar: @jrsbbq @heymanhustle @realcurtisaxel he wasn't successful as McGillicutty. What makes you think a name change will help?SMH
— Jim Ross (@JRsBBQ) May 25, 2013
Now I would agree that this is more than "a name change" considering JR mentioned Heyman's involvement which certainly cannot hurt. The fan who responded to him brings up an interesting point even if it is indirectly. WWE seems to think that they can simply choose to get someone over whenever they want. That is why we see guys debut, get pushed, start to get over, get de-pushed, treated poorly and then fail to get over in any meaningful way after that point. Jack Swagger is reasonably over now with his "Real American" gimmick and Zeb Colter being part of his act. He isn't all that over either though. I think in large part that has to do with being treated like a joke on RAW for so long. Jobbing to mid to undercard acts is not a recipe for helping a talent succeed. Michael McGillicutty being treated like dirt in his tag team with David Otunga isn't just going to disappear from the WWE Universe's minds because Paul Heyman speaks highly of him and he gets a new name.
Point is WWE needs to be more concerned with how they treat their talent onscreen. Treating them poorly for an extended period of time can seriously hurt later efforts to get those guys over. Sometimes WWE can overcome this, but then again often they cannot.


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The Galaxii Series Omnibus 1: Into The Abyss
Buy now as an eBook (epub) from The Crow Bar for only $5!
- Published: Fifth Edition, June 13, 2018
- Words: 188,061 (Flowing Text eBook)
Please consult the Refund and Privacy Statement pages if you have any questions before purchasing.
Economize! Buy "The Galaxii Series Omnibus 1: Into The Abyss", containing the first three titles in the Galaxii Series by Christina Engela: "Blachart", "Demonspawn" and "Dead Beckoning" – all in one big omnibus, for LESS than buying all three separately!
Do you want to know more?
Book trailer videos for Blachart, Demonspawn and Dead Beckoning:


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Phone (03) 8532 4444
A luxury swimming pool is the perfect finish to your outdoor living space, adding value not only to your home, but also to your lifestyle too. Canny Integrated Pools are part of the Canny group, and specialize in inground, concrete custom pools that fit perfectly with the architecture of your home.
Whether you are having a new home built in North Balwyn, or considering the addition of a luxury swimming pool to an existing property, Canny will ensure a stunning design, and a flawless finish. Our architects and builders are fully accredited, and highly experienced.
The latest 3D modeling technology allows you to visualize the completed design of your luxury swimming pool, before construction even begins.
We place emphasis on ultra-modern architecture that is both functional, and incredibly stylish. With our technical expertise, creativity, and extensive facilities, Canny integrated Pools can create a stunning custom design swimming pool that brings your home to life.
Our swimming pool building services in North Balwyn include:
To find out more about our swimming pool designs, luxury home building and custom architectural services in North Balwyn or to arrange a consultation – contact Canny today.
We also service: Armadale, Ashburton, Ashwood, Balwyn, Camberwell, Canterbury, Doncaster, East Hawthorn, Glen Iris, Hawthorn, Kew, Kew East, Malvern, Mont Albert, North Balwyn, Prahran, South Yarra, Surrey Hills, Toorak and most of Melbourne's Inner East
We'd love to hear from you!
Whether you're inquiring about a luxury home or you'd like to provide us with some feedback, please feel free to call us or fill out a contact form.


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Kingston Raiders – Kingston Frontenacs
Ever heard of the Kingston Raiders? No. You're not alone. In 1988, the Junior 'A' Kingston Canadians were bought by Lou Kazowski who renamed the club the Raiders adopting the silver and black of the NFL's LA Raiders. After a dismal season, the unpopular Kazowski sold the club to local hockey legends Wren Blair and Bob Attersley who renamed the club the Frontenacs, a name that had been used by a pro club that had played in Kingston in the EPHL. Pictured is a Raiders puck from their lone season along with a current Kingston Frontenacs puck.


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Air Temperature Sensor
High quality "NTC" type air temperature probe manufactured by Bosch. Overall length of 49mm, and a fitting thread of M12 x 1.5mm pitch. The resistance of the sensor changes with temperature. For direct connection to the DL1 a "pull up resistor" is required. If the sensor is ordered with a cable, this resistor is already included and will be ready for immediate use with the DL1 or DL2.
Technical Data
|Measuring Range
|-10°C to +100°C
|Permissible Temp
|max °C +130
|Electrical Resistance at 20°C (kohm)
|2.5 ±5%
|Electrical Resistance at:–10°C(kohm)
|+20°C (kohm)
|+80°C (kohm)
|Nominal Voltage (V)
|Measured Current,max (mA)
|Self-heating at max. permissible power loss P=2mW and static air at T=23°C (°C)
|Thermal time constant *1
|approx 20s
|Guide value for permissible vibration acceleration (sinusoidal vibration) (m·s-2)
*1 At 20°C. Time required to reach 63% of final value for difference in resistance, given an abrupt increase in air temperature; air pressure 1000 mbar; airflow rate 6 m · s-1
For further information on the air temperature sensor please visit our Knowledge Base here
* - Main product required.
Note: all prices are exclusive of VAT at 20.0%.


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Isle of Capri Casinos Appoints GM18 August 2006
ST. LOUIS, Missouri – (PRESS RELEASE) -- Isle of Capri Casinos, Inc. (Nasdaq: ISLE) officials announced today the appointment of Bari Richter as vice president/general manager of the company's Isle of Capri-Boonville, Mo. property.
Bari joined Isle of Capri in 2000 at the company's Marquette, Iowa property as senior director of marketing before transferring to Boonville in 2003 serving in a marketing capacity until she was appointed to the role of senior director of operations in 2004. Her prior experience includes extensive marketing, accounting and operations expertise including positions with Miss Marquette Riverboat Casino in Marquette, Iowa; Silver Eagle Casino Cruise in East Dubuque, Ill. and Dubuque Casino Belle in Dubuque, Iowa.
Bari holds a bachelor's degree in business from Linfield College in McMinnville, Oregon, as well as the executive development program from the University of Nevada at Reno.


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Effective project managers know how to provide strategic value by helping their organisation deliver on promises. They also recognise that having the right tools is vital to getting the job done right. Whether you need the basics like tools for creating schedules and tracking statuses, or you're looking at managing more complicated processes, Project Online can help. A crash course in Project Online explores how it enables you to:
- Deliver on time and within budget
- Identify risks
- Set milestones
- Assess the value of progress
No matter which critical path you're on, Project Online helps ensure that you and your organisation are successful.


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The day was almost over, I looked forward to the close of work after a hectic day at the clinic. The clock read 5:50pm. Ten more minutes I silently chanted to myself smiling delightedly. Ten more minutes and i could go home, relax, have a good dinner and surf the net. A perfect end to an incredibly busy and tiring day. Alas! It was not to be.
At precisely, 5:55pm, my boss called me to go on an ambulatory visit. To say I was upset was an understatement. All the same, I packed my things and went to see my patients.
On getting there, I discovered that there were 9 dogs – a mixture of Rottweilers and brindled Boerboels- all male.
Their handler called me aside saying, "doctor, there is one, hin tin don big." Not fully comprehending, I asked him to show me the one. I couldn't believe my eyes! It was the biggest scrotum I had ever seen on a dog. Up untill then, I hadn't witnessed a case that bad.
I examined him quickly. His temperature was sky rocketing, he could barely walk and was in obvious distress. It was painful watching him try to walk. I knew immediately that I had to take the poor dog back with me to the clinic.
I got to work treating the other eight dogs quickly. It was a routine treatment- deworming and vaccinations.
On getting to the clinic, every one was startled. "What is that?" – was the question on everyone's lips as they pointed at the poor dog. My colleague was almost convinced it was a hernia. It was that huge!
We put him on the table, examined carefully and properly and concluded it was indeed Orchitis- inflammation of the scrotum. Antibiotics and pain relievers were administered daily for a week. By the 7th day, our dear boerboel was ready to go home with a normal-sized scrotum.
His handler was so relieved when he came to pick him up and I learnt that sometimes great opportunities happen at the least favourable times.


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Medx Rehabilitative Exercise and FitnessAbout our facility
Medx Rehabilitative Exercise and Fitness is equipped with the industry"s most advanced and sophisticated exercise equipment. We feature Medx Exercise machines, the most technologically advanced and biomechanically correct equipment in the world.
Medx machines are the work of Arthur Jones, famed inventor of the Nautilus machines. After selling the Nautilus Company in 1986, Mr. Jones completed the design and development of sophisticated, computerized muscle strength testing machines to be used in medical rehabilitation and scientific research. He named his new company Medx. Medx equipment is the culmination of over 20 years of research and development and today stands as the ONLY VALID AND ACCURATE devices for measuring human muscular performance. Medx Rehabilitative Exercise and Fitness is one of the only facilities in the state of New Jersey that is equipped with these machines.
Upon completion of the "medical" machines, Medx incorporated the technology into a line of "exercise" machines. The Medx medical exercise machines have brought exercise training to a whole new level. They possess the most technologically advanced features unmatched by any other exercise equipment in the world.
Medx Rehabilitative Exercise and Fitness has spared no expense to provide its clients with this state of the art exercise equipment. Training on this equipment allows Medx Rehabilitative Exercise and Fitness clients the ability to maximize the quality of the workouts and optimize physical development in the safest and most effective manner possible. No other facility in the area, including any of the multi-million dollar health/sports clubs, matches Medx Rehabilitative Exercise and Fitness exercise equipment lineup.
Medx Rehabilitative Exercise and Fitness
Tel:Office 732-671-1430 Cell 917-701-6066


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From the February 12, 2009 issue of the Sopris Sun
CCAH winter dance party will bubble with Mountain Fair spirit
By Jessica Downing
Community Correspondent
The Carbondale Recreation and Community Center will heat up with the progressive soul and funk of Bumpus for the second annual Mid-Winter Mountain Fair on Saturday, February 14. Chicago-based Bumpus headlined Saturday night at Mountain Fair 2008 and is returning for what organizer Amy Kimberly is calling "the biggest indoor dance party Carbondale has seen."
"The Mid-Winter Mountain Fair just seemed a natural evolution once we had a venue for it," said Kimberly, program director for the Carbondale Council on Arts and Humanities (CCAH). "The joy and sense of community that comes from dancing together while raising money for CCAH is just too good to pass up."
Inspired by Sly and the Family Stone, the members of Bumpus came together 10 years ago playing in Chicago's Bop Shop.They have since played thousands of shows and put out four albums, intent on "capturing the spirit of soul while moving into the future of funk." They have opened for many national bands including Jurassic 5,They Might Be Giants and The Roots.
Opening for Bumpus will be local band Elemenopee.And great music isn't the only reason to attend. Multimedia artists Liquid and Lux, along with Jammin Jim Pomey and Carolyn Cipperly will be hosting The Interactive Chill Bubble filled with pillows and projections.
The Backstage Lounge – a la the Beer Garden – will serve up New Belgium Beer, Jack Rabbit Hill wine, and of course,the famous Mountain Fair mojitos will be on tap. Touching on the theme of Valentine's Day will be the"Art Vendors of Love" with art from Megan Larsen and Laura Vogel.
"The fact that Valentine's Day falls on a Saturday allows us to be playful," said Kimberly. "We will have Vicki
Browne's fresh-baked sugar hearts with personalized messages; fun, funky art created by local artists; and lots of cool ways to interact."
Tickets for the Mid-Winter Mountain Fair are available at Sounds Easy and CCAH for $25.
Tickets are available for CCAH members in advance for $20 by calling 963-1680.
Doors open at 7:30 p.m.
Mid-Winter Mountain Fair Who: Carbondale Council on Arts and Humanities
What: Live music by Bumpus, the Chill Bubble, "Art Vendors of Love"
When: 8 p.m. February 14; doors open 7:30 p.m.
Where: Carbondale Recreation and Community Center, 567 Colorado Ave.
Tickets: $20/$25 CCAH members/non-members Info: 963-1680


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Installing a green roof goes a small way to replacing the nature destroyed by building
However, there are plenty of good reasons to incorporate a green roof into the design of a building or to install one on an existing building.
Recent EU legislation outlines the need for
interlinked green spaces to be at the heart of future
housing and urban developments. The emphasis will
be on increasing biodiversity and improving quality
of life in towns and cities. This will be achieved with a
variety of design solutions incorporating green roofs,
green walls, managing open water, gardens and park
We can show you how installing your own green roof can:
- - Reduce energy consumption
- - Increase thermal insulation
- - Extend the lifespan of your roof waterproofing
- membrane by 400%
- - Reduce noise polution and create sound insulation
- - Reduce storm water runoff by up to 80%
- - Enhance photovoltaic performance
- - Preserve /creation habitat
- - Improve building air quality
- - Increase efficiency of solar panels
- - Increase biodiversity
- - Obtain planning gains
- - Reduce urban heat island effect
and will generally make your green roof more pleasing to the eye!


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An article published by the Atlantic describes Deputy Director of iiD Jerry Reiter's research program on methods for sharing data in ways that protect the confidentiality of data subjects' identities and sensitive attributes. This work comes out of grants supported by the National Science Foundation and is associated with two iiD projects that Jerry leads.
External Links:


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Super Simple, Customizable, Light Weight, Easy Text Rotator with Style
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The green environment of this place is really attractive for you.
The available 40 – 50 Bhk house which is with well-designed balcony, bedroom, hall, living room and kitchen.


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- Methodology article
- Open Access
HTJoinSolver: Human immunoglobulin VDJ partitioning using approximate dynamic programming constrained by conserved motifs
- Daniel E Russ1Email author,
- Kwan-Yuet Ho1 and
- Nancy S Longo2
© Russ et al.; licensee BioMed Central. 2015
- Received: 20 January 2015
- Accepted: 22 April 2015
- Published: 23 May 2015
Partitioning the human immunoglobulin variable region into variable (V), diversity (D), and joining (J) segments is a common sequence analysis step. We introduce a novel approximate dynamic programming method that uses conserved immunoglobulin gene motifs to improve performance of aligning V-segments of rearranged immunoglobulin (Ig) genes. Our new algorithm enhances the former JOINSOLVER algorithm by processing sequences with insertions and/or deletions (indels) and improves the efficiency for large datasets provided by high throughput sequencing.
In our simulations, which include rearrangements with indels, the V-matching success rate improved from 61% for partial alignments of sequences with indels in the original algorithm to over 99% in the approximate algorithm. An improvement in the alignment of human VDJ rearrangements over the initial JOINSOLVER algorithm was also seen when compared to the Stanford.S22 human Ig dataset with an online VDJ partitioning software evaluation tool.
HTJoinSolver can rapidly identify V- and J-segments with indels to high accuracy for mutated sequences when the mutation probability is around 30% and 20% respectively. The D-segment is much harder to fit even at 20% mutation probability. For all segments, the probability of correctly matching V, D, and J increases with our alignment score.
- Chronic Granulomatous Disease
- Mutation Probability
- Dynamic Programming Algorithm
- Alignment Score
- Random Match
Immunoglobulins (Ig) are a family of proteins that identify and bind foreign pathogens, e.g., bacteria and viruses. Diversity in the antigen-binding region of Ig provides an appropriate immune response to the wide array of pathogens confronting individuals. This diversity is generated by VDJ recombination, which joins a Variable (V) gene segment, a Diversity (D) gene segment, and a Joining (J) gene segment from distant regions of DNA to potentially create about 10 billion different antibodies, each of which binds to a distinct epitope.
During recombination, nucleotide excision of the germline gene termini and the addition of nontemplated N nucleotides by terminal deoxynucleotidyl transferase (TdT) at the V to D and D to J junctions provide additional diversification. Furthermore, during germinal center reactions B cell receptors undergo further changes including somatic hypermutation as well as nucleotide insertions and/or deletions (indels) of various length which creates a larger potential repertoire of antibodies.
Previously the JOINSOLVER [1] algorithm was used successfully to compare an unknown VDJ rearrangement against a set of V-, D-, and J-germline sequences to provide information about gene utilization in the Ig repertoire in a wide variety of conditions including: S. aureus immune evasion [2], CDR3 characteristics and VH mutations in systemic lupus erythematosus [3], immunological memory in chronic granulomatous disease [4], rheumatoid arthritis, [5], CDR3H characterization of the fetus and neonates [6]; X-linked HyperIgM [7], and the analysis of the neutralizing HIV antibodies [8]. Unfortunately, JOINSOLVER was not designed to handle indels.
This paper addresses the challenge of both accurately aligning heavily mutated Ig rearrangements, potentially with indels, to the nearest matching V, D, and J germline gene and identifying junctional N nucleotides. We introduce a sequence alignment algorithm that approximates the results of a dynamic programming (DP) algorithm, which can save up to 98% of the computational time.
Dynamic programming algorithms have been used to align sequences since 1970 [9,10]. Typically, DP alignment algorithms align sequences by creating a matrix with the rows corresponding to the bases of one sequence, and columns corresponding to the bases of second sequence. Matrix element (i, j) is the best alignment up to the ith base of the first sequence and the jth base of the second sequence. Dynamic programming algorithms have rules that define how to initialize the matrix, how to fill matrix elements after initialization, and where to find the highest score. A traceback is kept to mark the path of the best alignment through the matrix starting from the highest score matrix element. Most DP algorithms rules allow for insertions, deletions, mismatches, and matches. A match or mutated base is a one base step forward in both sequences, corresponding to a diagonal step in the DP matrix. Insertions and deletions increment one sequence, but not the other, corresponding to a right or downward step, respectively. Typically DP algorithms have poor performance, of order O(NM) where N and M are the lengths of the two sequences being matched [10]. Durban [11] provides an outstanding, in depth explanation of the use of DP algorithms for sequence matching.
Previous work suggests that banding, or working along a diagonal band in the DP matrix, improves the performance of DP algorithms [12]. In the same spirit, our method uses prior biological knowledge to lock down the alignment at highly conserved motifs in V- and J-germline genes and traverses along the diagonal of the DP matrix to significantly improve the speed and accuracy of alignments. When the motifs are not found, the algorithm falls back to a more traditional DP algorithm.
The alignment of the V-segment accounts for most of the computational work. The amount of work is related to the length of the segments and the number of sequences being compared against the segment. In germline database used by JOINSOLVER, there are many more V-germline genes (289) than D- or J-germline genes (84 and 12 respectively), and the V-germline genes have the longest germline sequences (~285 nucleotides) used in the analysis. The algorithm balances the need for aligning these irregular V-segments with the need to analyze large numbers of sequences provided by next generation sequencing. A new desktop application, HTJoinSolver, is provided as an implementation of the new partitioning method.
Partitioning sequences using conserved motifs
Next the partitioning method identifies the JH segment using a highly conserved J motif in a process similar to the VH alignment (Figure 1c). The remaining unaligned sequence is split just before the J-motif. A DP algorithm is used to align this fragment to J germline genes and to identify the 5' start of the J-segment. The V-end algorithm is used to align the 3' end of the J-segment. The two J fragment alignments are merged to produce a fully aligned J-segment. Finally, the D-segment is matched using a specialized local DP algorithm (Figure 1d) to produce a fully partitioned and aligned rearrangement (Figure 1e). Those nucleotides that are not partitioned with the VH, D, or JH segment are considered N addition nucleotides.
The approximate backwards algorithm
Unlike the DP algorithms commonly used for sequence alignment, the approximate backwards algorithm starts from a known point and works backwards to the start of the matrix formed by the germline gene and the unknown or query sequence. In this study, the scoring rules are +5 for a match and −4 for a mismatch; gap opening (indel formation) is penalized −30, and gap extension (continuation of indel) is penalized −1. The numbers in the matrix elements are the scores of the best alignment up to the bases corresponding to the column and row. The algorithm starts at the location of the 5' T of the conserved V-motif in both the germline sequence and the query Ig sequence. The score is initialized to zero. The algorithm steps one base backwards, toward the 5' end in both sequences. A traceback, which points to the previous matrix element, is kept to mark the alignment. As long as the bases match, the algorithm continues stepping in the 5' direction.
If the bases don't match, the algorithm considers the possibility of a mutation or an indel. The algorithm steps forward 10 bases (toward the 3' end) and calculates a small 40 × 40 DP matrix using an algorithm similar to the Needleman-Wunsch algorithm [9,10] to find the best alignment in this small section of the sequences. The small matrix is initialized by assuming inserts, which fill the elements in last column, and deletions, which fill the bottom row. Every other element in the small matrix is the maximum of a match, a mismatch, an insertion, or a deletion using the scoring rules. The tracebacks maintain the alignment through the small matrix. By calculating all elements of the small matrix, the algorithm determines the best alignment through the matrix taking insertions, deletions, mutations, and matches into account. The algorithm continues aligning from the highest scoring entry in first row or column of the matrix.
After completing the alignment, sequences with indels are re-aligned from 5' end of the alignment to the 3' end of the V-motif. The two alignments are compared for consistency. This quality control step catches suboptimal alignments caused by local maxima.
3' V-end alignment algorithm
After aligning the 5' end of the V-segment, we align the 3' of the V-segment starting at the conserved VH-motif. The V-end alignment algorithm, which is a type of overlap DP algorithm [11], begins at the first "T" in the VH-motif for both the query and germline sequences. A full matrix calculation is required, however the matrix is fairly small because only a few nucleotides occur after the V-motif in the V-germline genes. The 3' end of the V-segment is defined by the maximum score, which can occur anywhere in the matrix. The 5' J-end alignment uses the same algorithm, starting at the "C" from the conserved JH-motif.
J-start alignment algorithm
The start of the J-segment is aligned using a DP algorithm similar to a Needleman-Wunsch algorithm; however, the maximum score can be anywhere in the DP matrix, and the algorithm is run backwards from the J-motif. The maximum score corresponds to the start of the J-segment. The alignment is determined by tracing back from the highest scoring matrix entry.
D matching alignment algorithm
The D alignment uses a local alignment algorithm similar to a Smith-Waterman algorithm. A match is given a score of +1, however a mismatch sets the score back to 0. When the score = 3, the algorithm looks back 4 steps to check for a potential mutation, if the score before the match start is greater than 3, the current score is increased by the previous score. This allows a mismatch to be recognized as a mutation in the D-segment if and only if there are at least 3 consecutively matching bases on both sides of the mutation. The probability of randomly matching 3 bases is around 1.5% or 3% on either side of the D-segment, and 3 was chose to keep the probability of random matches below 5%. The termination condition is the maximum score anywhere in the matrix. These modifications to the Smith-Waterman algorithm maintain the basis of the original JOINSOLVER, matching D-segments based on consecutive matching bases.
Falling back to other algorithms
In the event, the V-motif is not found in the sequence, the algorithm looks for the motif with one mutation. If the motif with a single mutation is found, the approximate backwards algorithm is run using the mutated motif location. Because the J-motif is shorter, mutations are not allowed in the J motif.
When the location of the V-motif is wrong, the alignment is very poor resulting in a low score. Setting a score threshold of 3.1/base effectively catches an incorrect motif location. Before relinquishing to a slower algorithm, one last attempt is made to identify the location of the motif. The sequence is aligned to the first germline sequence in our database (IGHV1-18*01). The motif location from this alignment is used to align the query sequence to all other germline genes to determine the best match or alignment. If the highest scoring alignment is still below 3.1/base, we run an overlapping sequence DP algorithm for the V or a Smith-Waterman algorithm for the J alignments. Our only modification is that the end of the V- or J-segment occurs at the maximum score anywhere in the matrix, not just in the last row or column.
Default parameter used in simulations, unless otherwise mentioned
Start (5')
Random (usually 1)
End (3')
Mean Length
Two sets of simulations were performed. In the first simulation, 10,000 rearrangements were generated with mutation probabilities ranging from 0% to 95% in increments of 5% throughout the VDJ sequence. Using these rearrangements, the approximate backwards algorithm was compared against a complete DP algorithm, to calculate the success rate. The second set of simulations with 1,000 rearrangements engineered with a mutation probability of 3.5% was performed to compare HTJoinSolver with the original JOINSOLVER using the original JOINSOLVER germline database. The results of an alignment are considered a successful match to a germline if the algorithm returns the correct gene; the exact allele is not required.
Comparison with a standard dataset of biological VDJ rearrangements
Additionally 13,153 human sequences from the Stanford.S22 dataset [15] were analyzed to compare HTJoinSolver with several other frequently employed algorithms. Briefly, the Stanford_S22 data were produced by 454 sequencing of peripheral blood mononuclear cells from a single donor. The S22 individual genotype was determined using an individual analysis of iHMMune-align [16] results. The Stanford_S22 dataset and the S22 genotype form a standard dataset to evaluate the performance of VDJ partitioning algorithms. An online evaluation tool, Evaluation of IGH partitioning tools ([17], http://www.emi.unsw.edu.au/~ihmmune/IGHUtilityEval/evalForm.html) compares the performance of VDJ partitioning tools on the Stanford_S22 dataset. As a performance metric, the tool reports the percentage of V, D, or J assignments to germlines that are not present in the predetermined S22 genotype.
Germline database
The germline genes used in HTJoinSolver are IMGT reference sequences [18,19], www.imgt.org/download/GENE-DB/IMGTGENEDB-ReferenceSequences.fasta-nt-WithGaps-F+ORF+inframeP, downloaded July 30, 2014). HTJoinSolver provides methods to download and reformat germline genes from IMGT. These methods allow the user to maintain an up-to-date library of germline genes usable by HTJoinSolver. When comparing HTJoinSolver with JOINSOLVER, we used the JOINSOLVER germline database, which was derived from IMGT and included pseudogenes.
Access to HTJoinSolver
HTJoinSolver is available for public use, and can be downloaded from https://dcb.cit.nih.gov/HTJoinSolver.
The results of the HTJoinSolver approximate algorithm were then compared to the original JOINSOLVER V-alignment algorithm. Although the JOINSOLVER algorithm was not designed to identify indels, large portions of the rearrangement can align correctly up to the position of the indel, but the remaining V-segment was mismatched. Unfortunately, the results were not usable for mutation analysis because the offset alignment appeared as a region of high mutation instead of a single indel. Adding V-segment indels to our simulations hurt the overall performance of the original JOINSOLVER. With a 3.5% mutation probability and simulated indels, the original algorithm selected the correct V-germline 61% of the time, whereas the new algorithm selected the correct V-germline over 99% of the time. The percent of success for the old vs. new algorithms were 73% vs. 91% and 98% vs. 99% for the D- and J-germlines, respectively. These simulations show that the approximate backwards algorithm performs as well or better than the original JOINSOLVER algorithm.
The D-segment, which is relatively short, is the most difficult segment to identify and align. The D-segment nucleotides must be found within the CDR3, which is the most complex region of the VDJ rearrangement. In order to locate the CDR3 5' and 3' boundaries the algorithm must first correctly identify the 3' end of the V-segment and the 5' end of the J-segment whose nucleotide sequences may differ from the germline gene if nucleotide excision and/or mutation has occurred. N addition may be present or absent and varies in the number of nucleotides flanking the D-segment. Furthermore, D-segment mutations decrease the number of consecutively matching D-nucleotides. Thus, the D alignment success rate drops off almost immediately as the mutation probability increases. The curve in Figure 4 labeled "D Success Rate" levels off at around 4%, which is around what is expected by randomly matching D-germlines. The success rate drops from 93% at a mutation probability of zero to 89% at a mutation probability of 5%. The solid line with 'X' marks labeled "All Success Rate" corresponds to matching the full VDJ rearrangement. This curve strongly resembles the D success curve, reinforcing the fact that the D alignment is the most difficult and performance-limiting step.
The second column in Figure 5 is the success rate for the D-segment. The solid vertical line is at alignment score equals nine, marking the shortest acceptable D-score suggested in the original JOINSOLVER paper [1]. The effect of mutation is pronounced. As the mutation probability increases, fewer sequences have scores that cross the 9-base threshold needed to avoid random matches. When the mutation probability gets high, even though random matches of length 9 are rare, they occur as likely as real matches. Therefore, the success rate drops even at scores as high as 9. The J-segment success rates are shown in the right column of Figure 5. For low mutation probabilities, the J-segment is well identified. As the mutation probability increases to 20%, the success rate drops significantly for low scoring alignments.
Evaluation of IGH partitioning using inferred genotypes tool
Total (%)
3.60 (0.74)
2.30 (2.14)
0.42 (0.0)
All D
3.60 (0.74)
1.11 (0.99)
0.42 (0.0)
6.80 (0.28)
0.71 (0.59)
0.53 (0.0)
All D
6.80 (0.28)
1.25 (1.07)
0.53 (0.0)
High-throughput sequencing of B-cell receptor VDJ rearrangements produces vast numbers of sequences and requires extremely fast algorithms for VDJ alignment. The original JOINSOLVER algorithm was fast and accurate, but was not designed to handle indels. One of the main reasons for creating HTJoinSolver was to handle alignments with indels, while maintaining or improving the speed. Aligning a sequence to approximately 300 VH-germlines takes about 1.7 second on a 2.93 GHz Intel Core i7 iMac. Our approximate algorithm takes around 207 ms. Falling back to standard methods can take over 7x longer. One million lightly mutated sequences (simulation parameters in Table 1) took approximately 6 hours to run on 40 threads on a 4 × 12 core Opteron 6172 2.1 GHz computer.
The increase in speed occurs because we use the available biological information to avoid unnecessary calculations. We know that the highly conserved motif "TAT TAC TGT" begins at codon 102, and we know that insertions or deletions occur in less than 5% of the expressed repertoire, and are usually less than 10 bases [21]. Given these pieces of information, the algorithm calculates only a small fraction of the total DP matrix, even at high mutation probabilities. Our approximate solution was successful at replicating the score of a complete DP matrix.
When the motif is not found, the algorithm makes several attempts to align the sequence before falling back to the complete overlapping DP algorithm. However, without knowing the position of the motif, we lose the increased efficiency that comes with the use of this prior information.
A potential problem can occur if a sequence has a long stretch of mismatches that is longer than the rectangular block matrix used to determine if a mismatch is a mutation or an indel. The algorithm may incorrectly fall into a local maximum, (i.e. not seeing enough of the sequence to find the actual best alignment) and may be incorrectly aligned. Usually, the score threshold will catch the incorrect alignment, and fall back to the overlap DP algorithm. If it is known beforehand that long stretches of indels or mutation will occur, increasing the block size may improve the performance. The larger the block size, the less likely the algorithm will fall into a local maximum requiring a fall back algorithm. However, the price for a large block size is increased computation time.
We were not surprised by the comparison between the original JOINSOLVER and HTJoinSolver. We wanted to show that the HTJoinSolver can perform complete alignments of VDJ rearrangements containing indels. Therefore, all of our simulations included indels, which hindered JOINSOLVER's performance. The increased success rate of the improved algorithm is due to the ability to identify the size and position of indels. The D and J results were very similar because the DP algorithms used to align the D and J regions resemble the original JOINSOLVER algorithms, which were designed to prevent random D matching at the 95% confidence level [1].
The ability to successfully match a sequence to a germline is heavily affected by the length of the V-, D-, or J-segment and the mutation probability. Matching V-segments is easiest because longer germline sequences are available for comparison. As the mutation probability increases, the success rate drops. Both of these cases can be explained by the loss of information from the original germlines by truncation and mutation. Additional studies on whether the success rate can be explained by a noisy-channel model could give additional insights into the theoretical performance of any alignment algorithm.
Germline sequence similarity presents an additional challenge for HTJoinSolver, especially for aligning heavily processed sequences. The problem is compounded by the fact that the D-germline sequences can be similar to the 5' end of the J-germline sequences. A small fraction of our simulations show a VH-JH distance of zero, even though we know a D-germline is present along with VD and DJ junctions. This ambiguity cannot be resolved without additional information.
When compared against the original JOINSOLVER algorithm using the Evaluation of IGH partitioning using inferred genotypes tool, we see an improvement in the V, D, J, and overall results. However, the improvement is small because the sequences are minimally mutated. The median mutation frequency for the S22 dataset is 0% and the mean is 0.9%.
The results of the evaluation tool are highly dependent on the set of germlines used in the comparison. When compared against other partitioning tools using the original JOINSOLVER database, the results show that HTJoinSolver makes fewer V, D, and J assignment to germlines and alleles not present in the S22 genotype than the tools in the study by [17]. When the germline database was updated, the resulting evaluation changed. More V-alleles not in the S22 genotype where matched, hurting V and total performance.
The development of an evaluation tool with a standard dataset is an important first step, but without a better method of assessing the quality of results, and a standard library of germlines, comparison between partitioning tools remains difficult. We have shown that for the original JOINSOLVER® database, always including the highest scoring D alignment, even when we would not recommend using the alignment, improves the partitioning performance according to the evaluation tool. Furthermore, providing a D-segment that occurs in the S22 genome will always improve the performance as measured by the evaluation tool, even if the D-segment is very likely to be wrong. Relaxing the criteria for including a match (e.g., allowing low quality D-matches) should not have improved results of the evaluation. After updating the germline database, the performance for the V-segment dropped. With more allelic variants to choose from, the system is more likely to match previously unknown allelic variants causing the evaluated performance to decrease. Improvements in the D alignment can be explained by the removal of the reverse germlines from the database when we switched from the JOINSOLVER germline database to the IMGT reference database for HTJoinSolver. Interestingly, always including the highest scoring D-germline, regardless of whether the score is high enough, does not improve the D-segment performance using the updated database. Evaluation results for the same algorithm changed because of differences in the germline database and not on algorithmic differences.
These results suggest that evaluating a partitioning tool based solely on whether or not a germline occurs in an assumed genotype is not sufficient. Consider a partitioning algorithm that only assigned IGHV1-3*01 to all query sequences. Since IGHV1-3*01 is in the S22 genome, 0% of the sequences would come from outside the S22 genome. The partitioning algorithm would be evaluated as perfect. However, this algorithm would not make a useful tool. Whereas the evaluation tool is a good idea, currently the tool is too sensitive to similarity between the S22 genome and the germlines present in the partitioning tool's database.
All of the simulations and the comparison with the partitioning tool evaluation software show that HTJoinSolver can identify V-, D-, and J-segment to very high accuracy for reasonably mutated sequences. As the mutation probability increases, alignment becomes more difficult but problematic only for unnaturally highly mutated or truncated sequences. The V-segment is identified very accurately for most biologically relevant cases. The J-segment is fairly well identified from 0-20% mutation probability but above 20% the success rate falls below 90%. At the 20% mutation probability, the D-segment has about a 55% chance of being correct. Regardless of the partitioning software, biologists should be skeptical of short D-matches to highly mutated sequences, even if mutations are not seen in the D.
The authors wish to thank Dr. John Mascola for the opportunity to develop HTJoinSolver. This work was supported by the Intramural Research Programs of the Center for Information Technology [DER, K-YH], National Institutes of Health and the Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health [NSL].
Authors' Affiliations
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This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.


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Last fall my daughter showed an interest in raising chickens, so began the journey..............
I researched and learned a lot about chickens, and found this website and became amazed at all the different chicken coops! So i took a little of this and a little of that from a few coops that i liked and came up with my own design. I did not create any plans to go by and pretty much just designed it as i went along.
Since it was the beginning of winter i started the coop inside,
Everything was prefabbed inside, so it could be put together like a puzzle outside. The coop is 4' x 8', raised two feet off the ground. The coop was framed for six nest boxes but only 4 will be available to chickens, the other two will hold supplies, tools, etc. I wanted windows for ventilation and to increase the overall look since my wife was not 100% on the chicken band wagon, i didn't want it to be an eye sore in the yard. Due to the high cost of windows i made my own out of plexiglass.
I did not want to be in a hurry on finishing the project, and figured we would not get the chicks until spring of 2015, unfortunately a friend had hatched 5 new black australorp chicks in January, and guess who became the proud owners?, so the project had to kick into overdrive!
i created the run to be 12' by 16', with a roof to provide shade, i installed 2x4 welded wire around the coop and buried it to keep predators out
I installed two big doors at the back of the coop for ease of cleaning and also installed two poop trays under the roost to collect the free fertilizer.
Things left to do ,
Install chicken access to the coop from the run
paint coop
install epoxy to protect wood floor
install automatic feeder and waterer
finish trim
The "Egg Factory"
By btownchix · Feb 12, 2015 · ·
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July 24th
On this particular date my brother and dad saw a baseball game in Philadelphia in which my mum and I went off to our own adventures.The drive from New Jersey to Philadelphia was about two hours we left rather early so that we could have a full day to see the sites
We first visited the Liberty Bell. It dates back to 1752 and is really important in American History and a symbol for American Independence.Its worth seeing if you are in Philadelphia! My mum and I then went on to find the famous L O V E sign which is located in the L O V E park and of course had a picture taken right in front of the sign. A typical tourist thing to do.
After we had some lunch at a lovely Irish restaurant and yes they did have free Wi-Fi too we went onto to see the famous Rocky sign and climbed the steps.The Rocky statue is located just outside the Philadelphia museum of Art. To climb to the top you have to climb 72 steps but once up the top its an amazing view of Philadelphia.
There's still so much left to see in Philadelphia and hopefully I will return to this beautiful city 🙂


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You know you're in trouble when the entrance to the next stage looks like a mouth.
Run, run you little grunt!...
Uh oh! That can't be good. XD
Love that little derpy face the cave has! lol
What misfortunes shall befall our orange hero??
Hahaha, thank you! I love to draw derpy little faces. ( ˙꒳˙ )


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PDA Quest Part II: A Pyrrhic Victory (2017/01/08)
Serious Sam came in the mail. Everything was going smoothly. The game files can be grabbed right from the disc without bothering with any dated PC software, so that's good. But the PC emulator refused to work. It's just too damn fast. When the back of the box said "Pentium 233MHz or better", I don't think a 5th gen i5 is what they had in mind.
So, it was time to get creative.
limiting POSE's cpu usage
My first though was that I could force the program to slow down. There are a few programs that can do this, most notably "Battle Encoder Shirase" which has a pretty funny name. Unfortunately, BES couldn't properly hook the executable, so it was completely useless. There are a few other options, like CpuKill, which attempts to slow down programs by running a literal fork bomb. I wasn't going to do that.
running POSE in its native habitat
According to the readme file, the version of POSE I found was released in 2001. Of course, it does not officially support Windows 7. XP compatibility mode didn't do anything, so my next step was to install a full Windows XP virtual machine to run POSE in. It may have worked once, but generally speaking, it was still way too fast. And way too black and white.
running POSE in its native habitat with some extras
Even limiting the VM's cpu usage until it was so slow that the Windows XP UI lagged (as if that's hard) did not stop POSE. This emulator sucks.
Installing Windows ME on an old laptop, or buying a PDA, or something
Thank god I didn't have to go through with this, because then I remembered something completely different: PHEM.
As it turns out, some other sad soul out there cares about the state of Palm OS emulation, and he ported POSE to Android. His port's called PHEM, and it was last updated in 2014, which makes it 13 years more recent than POSE. That's great, because it means I don't have to fiddle with any more old hardware. Assuming the underlying POSE code doesn't ruin everything, that is.
I'm not going to pretend PHEM is something it isn't: the Android code is trash and the interface sucks. Roms have to be placed in an obscure place on the device and it's still way too fast, depending on your phone hardware.
That being said, it works! PHEM runs the color version of the game with no errors, aside from the speed issue. The next problem is to find a way to mirror my phone's screen to a PC so I can record gameplay footage, but that is an entirely separate nightmare. In the meantime, here are some screenshots.
So, with all that work done, how's the game? It's garbage. Completely awful. The controls are unresponsive in ways I never thought possible and the level design sucks. But honestly, who cares? The levels are vague facsimiles of the ones in the PC version which is pretty cool to see, and it's one hell of a novelty. At least, it was before I dumped it online. Now it's just another shitty PDA game.
You can download Serious Sam TFE, as well as the necessary OS files, a copy of POSE to play around with, and some other files I mentioned earlier here. You can download PHEM to play it here, but it'll cost a dollar.
One Man's Quest to Play an Old PDA Game (2016/12/30)
In the last few years, I've gotten into playing first person shooters on the Gameboy Advance. They're all somewhat awful, but the biggest disaster of the bunch (and therefore my favorite) is Serious Sam Advance. I actually like this game enough that I've taken to speedrunning it, but I can't deny that it's a pretty bad game. It's also a game most people don't know about, since most people are only familiar with the PC shooters and not the bizarre spinoffs.
As it turns out, there is a game in the series worse than Serious Sam Advance. Maybe. Someone at Croteam (or maybe their old publisher Global Star) back in the day had the bright idea of creating a unique entry in the series for Palm OS. Yes, the Palm OS that ran on PDAs in the early 2000s. Apparently the game has fifteen levels, twelve enemies, and six weapons. It's also likely a loose adaption of The First Encounter, judging by its name and the fact that the first level is titled 'Hatshepsut.' Aside from that, I don't know anything.
Looking around on the internet, the above information dump seems to be about all anyone knows. There's no gameplay footage on Youtube, and only the demo is available for download anywhere. There's a guy on Ebay who wants $50 for the game but aside from that it seemed almost completely lost. That is, until I had the chance to buy it for $15. I took that opportunity before I even considered how I'd play it. Fantastic! I'm now the owner of an undumped video game.
I had just spent nearly $20 on a 15 year old PDA game, and I wasn't going to spend another $20 on the old PDA itself if I could help it, so I started looking for an emulator. As it turns out, there's an official Palm emulator that was used for development. I don't trust bloated old sites like cnet and Softonic, so finding a download for that was harder then I expected. Then, of course, the emulator needs to be given the OS rom itself, which you could probably have gotten from your PDA or Palm's website when it still existed. Luckily, I was able to find the exact rom version I needed (OSv3.5) pretty quickly.
The next thing to do was to test the emulator with something. The first thing I found was 'SUPER_MARIOLAND2.pdb', a dysfunctional half of what couldn't possibly be the real Super Mario Land 2. The next thing I tried was the Russian freeware version of a crappy RPG called Quest of the Hero, which actually worked just fine. After that, I remembered that the Serious Sam demo was actually available online.
Unfortunately, the demo doesn't work. Whenever it tries to load the first level in the color or the black and white version, we get this:
Which doesn't bode well for the full version. After some fiddling with different versions of the OS rom, I was finally able to get the B&W version in-game!
...For a little while, at least.
Ultimately, I think the issue is that the game is running too fast for its own good. What makes me think this is that the credits scroll way too fast to be readable, and the controls in-game are way too strange to be right. Very solid evidence indeed. This doesn't seem to be an emulator-wide issue, since Quest of the Hero worked fine, so the problem may be that Serious Sam specifically doesn't like to be emulated.
Maybe things will be different when it's time to run the full version of the game. Or maybe I'm in the market for a fifteen year old PDA. Either way, I've come too far to give up, and I'll be pissed off if I can't play the worst ad most obscure Serious Sam game ever released.


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Posted on June 03, 2014
Preheat the oven to 190 degrees.
In a large skillet over medium heat add olive oil, garlic and jalapenos. Sauté until garlic starts to brown. Reduce heat to medium-low, add spinach leaves and sauté until wilted. Add 1 tsp Slap Ya Mama Original Blend Seasoning, pepper jack cheese and sour cream. Stirring continuously, cook mixture until cheese is melted.
On a clean surface, lay chicken breasts flat and season with the remaining 2 tsp of Slap Ya Mama Original Blend Seasoning. Spoon a little spinach mixture onto each breast. Roll up each breast with spinach mixture inside and wrap each breast with 2 or 3 slices of bacon, securing the ends with a toothpick.
Arrange breast on a baking dish. Bake uncovered at 190 degrees for35 minutes. Then set oven to broil and broil for 10 minutes or until bacon is browned. Remove toothpicks, slice into medallions or serve whole and enjoy!
Servings: 4


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You and I are on the roller coaster of life everyday. We ride up and down, in and out; we seemingly have no control on where we are going. Sometimes we are silent, and sometimes we scream. Sometimes we hold on tight, and at other times, we let go and are carefree. I do not know about you, but I can tell you, there are times when I simply want to get off the ride. I want smooth sailing, not all the twists and turns. I want to know what is coming next, and not to be fearful of the unknown.
In our text for Sunday, Psalm 22, we are reminded of the roller coaster of life in a poignant way. This is a personal lament psalm, and it is the one Jesus quoted from the cross with those piercing opening words, "My God, My God, why have you forsaken me?" I shudder each time I hear those words because the feeling of abandonment is perhaps the most painful in the human experience.
The good news of the Psalm and of our existence, however is that we are not left alone, though we may feel like we are. Immediately after questioning God's presence, the psalmist is comforted in God's faithfulness throughout the generations. That up and down, back and forth is exactly the way we live today. Sometimes we feel God's presence, and at other times we feel completely alone. Sometimes we are close, and other times far away. What can we take from this?
Perhaps the most comforting claim is found in verse 24. The Psalmist exclaims, "for he has not despised or disdained the suffering of the afflicted one; he has not hidden his face from him but has listened to his cry for help." In other words, God hears our prayer each time, every time. But that is not all...we can be comforted by his presence even when we are not feeling it. Our feelings are a poor barometer of God's faithfulness. We are called to trust in that we do not see, and sometimes that we do not feel...that is what faith truly is.
So I challenge you to hold on tight to the ride. Sometimes up, down, left or right, but never alone, and never without hope.
May God bless you and keep you,


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Adoption Doula
Whoever has heard of an adoption doula? Now you have. I can help your growing family when you're adopting a newborn baby. I can give you the help and support you need to make your adoption process the best experience possible.
Why Have a Doula for Adoption?
In addition to being a doula, I'm also an anthropologist. As an anthropologist, I'm educated and prepared to understand and address your unique needs and concerns. Understanding your situation and needs is paramount when it comes to any blooming family. I take the time necessary to understand how and where help is needed to prevent any issues that may arise from the newborn adoption birthing process.
Did you know that adopting families can breastfeed? This is only one of many situations I can help you with.
It's a Group Effort
When I work with you in your newborn birthing process, I'll also work with the birthing parent to ensure that everyone is on the same page in all areas. This includes how to begin breastfeeding and transitioning this and more to the adopting family.
LGBT & More
Families of all types can benefit from my adoption doula services. You can learn more about my LGBT doula services as well.


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Panthers Thursday Injury Report (Cam Limited and Butler a Full Go)/
Teams are required to release three injury reports in the week leading up to game day. Teams playing Sunday must report injuries and practice participation reports on Wednesday, Thursday, and Friday. Carolina reported the following from Thursday practice:
Notables: Cam Newton was full participant and limited on Thursday. Looks like they are going to be cautious in not putting too much work on that shoulder. Vernon Butler was full participant in practice for the second day. It would be nice to have the big fella on the field with Shady coming.


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E-procurement system ProZorro helps save UAH 4 bln of budget funds
16:42, 20 September 2016Economy
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Cost savings due to the launch of the ProZorro electronic public procurement system, which is mandatory for use by all public agencies from August 1, have amounted to UAH 4 billion, according to the system's website.
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Pathology of the Week – April 9th
Jervell and Lange-Nielsen syndrome. Medicalese: first described in 1957 by Anton Jervell and Fred Lange-Nielsen. Short forms: JLNS or J-LN syndrome
JLNS is the first in a weekly series of uncommon pathologies I will post. I first heard about this cardioauditory (two words not usually together) disorder from a February, 2010 Dr. Venkatesan blog post. Jervell and Lange-Nielsen syndrome (JLNS) is a genetic disorder that affects potassium channels in the heart and inner ear. This affects cellular repolarization and lengthens the cardiac action potential causing prolonged QT intervals and neural deafness. JLNS is the most severe variant of the congenital long QT disorders with over 90% of patients becoming symptomatic and 25% suffering sudden death. In a sample of 186 patients 50% had a cardiac event before age three and only 5% neared adulthood symptomless. See this Circulation article (free access) as well as this thorough review by Tranebjaerg et al.
Signs and Symptoms
JLNS is usually diagnosed after a congenitally deaf child experiences repeated syncopal episodes at a young age. The two cardinal symptoms are profound hearing loss present from birth and a wide QTc interval (at least 500 ms). Long QT disorders predispose a patient to deadly cardiac arrhythmias such as Torsades, Vtach and Vfib.
JLNS is an autosomal recessive birth defect. This means both parents must be carriers of the mutated sexless (autosomal) gene. The above study did find, in 186 patients, a consanguinity rate of 35%. This greatly increases the risk of recessive genetic disorders. The mutation occurs on genes which would produce proteins that coassemble to form specific potassium channels in cardiac and inner ear cells. These cells normally pump positively charged potassium ions back out of the cell to aide in repolarization.
Management and Treatment
JLNS has an extremely poor patient outcome with a mean life expectancy of around 8 years. Pharmacologically beta-blockers are the most commonly prescribed drug. Unfortunately 51% of patients will still suffer a cardiac event (arrest or sudden death). Mean age at death for patients on Beta Blockers remains just 8 years. Cochlear implants are effective in restoring hearing and do not affect pacemaker activity. Patients are also usually advised to avoid activities that would stimulate sympathetic arousal of the heart such as sports, scary movies and even cold water.
Implications for Prehospital Care
This disorder is relatively rare, the authors of the 186 patient study had to assemble the sample from over a dozen countries in numerous continents. JLNS is something to consider for a differential diagnosis of a syncopal paediatric patient with a history of hearing loss at birth. It is also helpful in learning the long QT disorders.
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Airlight Energy, a Swiss-based supplier of solar power technology has partnered with IBM Research to bring affordable solar technology to the market by 2017. The system can concentrate the sun's radiation 2,000 times and convert 80 percent of it into useful energy to generate 12 kilowatts of electrical power and 20 kilowatts of heat on a sunny day — enough to power several average* homes. The High Concentration PhotoVoltaic Thermal (HCPVT) system, which resembles a 10 meter high sunflower, uses a 40 square meter parabolic dish, made from patented fiber- based concrete which can be molded into nearly any shape in less than four hours with mechanical characteristics similar to aluminium at one-fifth the cost.web: www.zurich.ibm.com/dsolarPublished on Sep 23, 2014 on You Tube by IBM Reasearch
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The WATCH Project played host to a laughter workshop run by Denise recently. One of the members who participated (Maureen) collected her thoughts on paper and was happy to share them on the website today:
"After a leisurely lunch (slow because so many bits I had to pick out)! Already some of the others were assembling in comfortable chairs at the other end. I didn't mind sitting in the middle of two people on a sofa to do that, hopefully I could "lip read." Nevertheless, I feel apprehensive as I had never been able to hear a talk.
However, I was told, right at the beginning that the speaker knew me. I was amazed – but it was true. She had heard about me from Simon Heyes when he was a resident at Swallowed cliffe. She remembered the morning we set off to the Brighton Mind Conference – Simon with sixty photocopies draped over his arm – Our first 1st S.U.S newspaper.
But on the talk – with 100% audience participation, I felt excited – Somebody I had known!
Ho, ho, ho hah – and we all stood up – eager to respond. Expelling air seemed to exhilarate me and I felt eager to follow the next direction. Shake hands – it felt good to feel the warmth of other people's hands and shaking them about really made me feel light-hearted and free as a bird.
Then she produced a soft yellow laughing ball and I couldn't help laughing with it and feel uplifted with abandonment. I didn't care about making a fool of myself. The laughter was infectious.
Then we chatted in gibberish like small children – crazy but infectious. I didn't feel embarrassed – free as a bird and didn't hesitate to converse in talking absolute rubbish!
We then dipped our hands into a bag, covering them with an imaginary powder. It felt good, even though nothing was there!
We then blew bubbles from tiny polythene pots, enjoying with wonder as they flew in the air.
Seeing Denise with an enormous "bubble maker" we looked around with wonder, no embarrassment or inhibitions, enjoying seeing each other totally relaxed – feeling a release of tension in each other.
I watched several latecomers walk in – the tension in their faces. I felt sorry they had missed the best talk. However, no straining to hear. Her voice came through loud and clear – a happy voice and I felt like communicating – the barriers had been lifted!"


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Dentures in Bethpage
Augenstein Family Dentistry Offers Workable Solutions
At Augenstein Family Dentistry, we know that when you are missing teeth, you may have difficulties with chewing and speaking, and it may even affect your self-confidence. Dr. Jay Augenstein, our experienced Bethpage dentist, has provided many patients with customized dental solutions for missing teeth, and he would be happy to help you, too. Our office is clean and friendly, and we offer punctual appointments, professional staff, and a personalized approach to your dental health.
If you have been considering dentures, call Augenstein Family Dentistry at (516) 589-7729 and schedule a visit.
Get Natural-Looking Teeth at Augenstein Family Dentistry
Augenstein Family Dentistry cares about keeping you informed. We will discuss all options for treatment with you and determine if dentures are the right route to take for your oral health. Properly fitted dentures can aid in your ability to chew foods and swallow, improve your speech, and even boost confidence. Typically, conventional dentures are placed after the gums have healed following a tooth extraction, but we can also provide you with immediate dentures for a temporary solution.
Why Choose Augenstein Family Dentistry?
We strive to provide outstanding customer service and trusted dental care to every one of our patients. Augenstein Family Dentistry has served the community of Bethpage and surrounding areas for more than 50 years. We offer emergency dental services to deal with urgent matters promptly. Our office accepts most major insurance plans, and we also extend financing through CareCredit.
If you are considering dentures or you need a repair or adjustment for your existing set, contact Augenstein Family Dentistry in Bethpage at (516) 589-7729.


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The family and I are really looking forward to seeing ANT-MAN AND THE WASP when it hit theaters soon. We've been going to the movies every weekend for the past couple of months because SO many great movies are coming out. This one is NO different!
From the Marvel Cinematic Universe comes ANT-MAN AND THE WASP, a new chapter featuring heroes with the astonishing ability to shrink.
In the aftermath of CAPTAIN AMERICA: CIVIL WAR, Scott Lang grapples with the consequences of his choices as both a Super Hero and a father. As he struggles to rebalance his home life with his responsibilities as Ant-Man, he's confronted by Hope van Dyne and Dr. Hank Pym with an urgent new mission.
Scott must once again put on the suit and learn to fight alongside the Wasp as the team works together to uncover secrets from the past.
ANT-MAN AND THE WASP is directed by Peyton Reed and stars Paul Rudd, Evangeline Lilly, Michael Pena, Walton Goggins, Bobby Cannavale, Judy Greer, Tip "T.I." Harris, David Dastmalchian, Hannah John Kamen, Abby Ryder-Fortson, Randall Park, with Michelle Pfeiffer, with Laurence Fishburne and Michael Douglas.
Check out this new featurette
- Like ANT-MAN AND THE WASP on Facebook
- Follow ANT-MAN AND THE WASP on Twitter
- Follow Marvel Studios on Instagram
Marvel Studios' ANT-MAN AND THE WASP opens in theaters everywhere on July 6th!


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Duke quarterback Thomas Sirk to transfer for his final season
Duke's Thomas Sirk on the Blue Devils' bowl win
Thomas Sirk will complete his college football career – one that's seen historic accomplishments intertwined with crushing injuries – at a school other than Duke.
The 6-4 quarterback, who has suffered three torn Achilles tendons since enrolling at Duke in January 2012, plans to transfer for his final season of eligibility.
Named co-Most Valuable Player of the 2015 Pinstripe Bowl when he led the Blue Devils to their first bowl win since 1961, Sirk already has his Duke degree and will be eligible to play this season at the school where he transfers.
"I am beyond thankful for my time spent at Duke University," Sirk said. "I will never take for granted the relationships I've built with the entire Duke community, especially my teammates and coaches. The men in the locker room have become my family over the course of five years, and calling them teammates would be an understatement."
Duke's release restricts Sirk from transferring to another ACC school and also prevents him from landing at nonconference schools the Blue Devils will play this fall – Army, Baylor, N.C. Central and Northwestern.
Regardless, he'll leave with Duke coach David Cutcliffe's blessing and admiration.
"We are 100 percent supportive of Thomas and his decision to complete his eligibility at another school," Cutcliffe said. "I've said it many times before: Thomas is an incredible young man. He's conscientious, dedicated and what I would consider the ultimate teammate due to his selflessness and leadership qualities. Thomas loves Duke. He and I have had great discussion regarding this decision, and his love for this university and everything it represents is what made this decision so difficult for him."
Sirk played only two healthy seasons at Duke, spending 2014 as the backup to Anthony Boone and 2015 as the Blue Devils starter. But he still established two school records.
After twice tearing his left Achilles tendon last year – in February and August – he sat out the season while recovering from the surgeries. Redshirt freshman Daniel Jones became Duke's starting quarterback. Even though the Blue Devils went 4-8 overall and 1-7 in the ACC, Jones completed 62.8 percent of his passes while throwing for 2,836 yards with 16 touchdowns and nine interceptions.
Jones threw eight interceptions in his first five games and one over the final seven.
That improvement has him Duke's clear No. 1 quarterback as the Blue Devils go through spring practice this month, which Cutcliffe has often stated.
Sirk has been at Duke's spring practices but is not taking part in contact drills. Because of his numerous injuries, the NCAA granted him a sixth season of eligibility in November that allows him to play in the 2017 season.
Sirk is on track to receive a master's degree in liberal studies from Duke this spring. He received an undergraduate degree from Duke in political science last May.
But, with Duke building its offense around Jones, Sirk is saying goodbye to the school and the football program he loves.
"I am grateful that Coach Cutcliffe believed in me to play quarterback at the next level when not very many people did," Sirk said.
Sirk took a healthy redshirt in 2012 before suffering his first torn Achilles tendon during spring practice in 2013. He had surgery and sat out the 2013 season.
In 2014, he played extensively in Duke's short-yardage packages, rushing for a team-best eight touchdowns as Duke went 9-4 and played in the Sun Bowl.
As the starter in 2015, Sirk again rushed for eight touchdowns while leading the Blue Devils in rushing with 803 yards. He also passed for 2,625 yards with 16 touchdowns and eight interceptions.
In Duke's 44-41 overtime win over Indiana in the 2015 Pinstripe Bowl, Sirk threw for 163 yards and a touchdown and rushed for 155 yards, a Duke bowl game record, and two touchdowns.
Sirk also set a Duke record for most consecutive pass attempts without an interception to begin a career with 93.
Steve Wiseman: 919-419-6671, @stevewisemanNC


text: Far from the shine and reflective characteristics of a stainless steel wire mesh look typically found in a modern urban scene, is a home remodel project in lake country Wisconsin. Restoration was the theme and a 3" square opening wire mesh fit the bill. The large .250" diameter wire was crimped and then woven into an intercrimp pattern to create a texture for this living space that no other material can achieve. The wire mesh railing infill panels were fabricated in a strong 13 gauge U-Edge frame, and by leaving the corners untouched after welding, these panels have the overall desired look the homeowner was after.


text: Contact Us Brechbill & Helman Construction Company, Inc.1115 Sheller AvenueChambersburg, PA 17201Phone: 717-263-4621Our office business hours are:Monday-Friday8:00am-4:30pmContact UsYou may contact us by filling in this form any time you need our professional services or have any questions. You can also fill in the form to leave your comments or feedback.Name:* First Last E-mail:*Phone:* Area Code - Phone Number Subject:*How did you hear about us?*Brechbill & Helman WebsiteFacebookLinkedInTwitterWord of MouthPreviously Worked On A ProjectCrossroads Business Journal AdDetails:*Send a copy of this message to yourself: Please Check Box BelowSubmitReset* Indicates required fields ShareTweet0 Shares


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Joss turned to art at the age of 58 and is now a worldwide selling artist with her work currently displayed in galleries in California, Virginia, Maryland and Indiana USA. She experiments with different textures and techniques including oil pastels, acrylics, gouache, inks, food colouring and scratching.
Her art is heavily influenced by her upbringing in South Africa. Her colourful work, often distorted and fragmented is impactful and beautiful. She has many private collectors worldwide and all her work is available as both Giclee and canvas prints. Her vibrant Note Cards can be ordered directly from her and are also in stores in the UK, USA and Kenya.
Copyright (c) Nude Tin Can Ltd. All Rights Reserved.
Registered England Company 09718791
125 Hatfield Road, St Albans, Herts AL1 4JS
Tel: 01727 569291


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Summer Sunshine and Apricot Vanilla mean warmer days filled with fun, freedom and fragrance. A sultry Midnight in Curaçao, a stay in a Rainforest Bungalow or lessons learning the Kahiko Hula. Our Scentsy 2018 Spring Summer catalog is the best in years!
This season, we're serving up a summer holiday for your Crazy, Hot Mess life! Awaken your senses with inspiration from Around the World. Whether you've been Lucky in Love for a dozen years or find yourself Accidentally in Love this season, we hope you find your Scentsy sunshine in 2018. Starting March 1, 2018 you may purchase these NEW and exciting products from the Scentsy 2018 Spring Summer Catalog and have them shipped directly to your home. Our customer service is second to none and our promise to you is that you will be 100% satisfied.


text: <!-- The RIOT community cares a lot about code quality. Therefore, before describing what your contribution is about, we would like you to make sure that your modifications are compliant with the RIOT coding conventions, see https://github.com/RIOT-OS/RIOT/wiki/Coding-conventions. --> ### Contribution description This PR update the params definitions scheme for the adxl345 device driver. <!-- Put here the description of your contribution: - describe which part(s) of RIOT is (are) involved - if it's a bug fix, describe the bug that it solves and how it is solved - you can also give more information to reviewers about how to test your changes --> ### Issues/PRs references Initially done in #7937 and related to #7519 <!-- Examples: Fixes #1234. See also #5678. Depends on PR #9876. Please use keywords (e.g., fixes, resolve) with the links to the issues you resolved, this way they will be automatically closed when your pull request is merged. See https://help.github.com/articles/closing-issues-using-keywords/. --> You can view, comment on, or merge this pull request online at: https://github.com/RIOT-OS/RIOT/pull/8671 -- Commit Summary -- * drivers/adxl345: use new driver params scheme * sys/auto_init: update adxl345 auto_init -- File Changes -- M drivers/adxl345/include/adxl345_params.h (9) M sys/auto_init/saul/auto_init_adxl345.c (11) -- Patch Links -- https://github.com/RIOT-OS/RIOT/pull/8671.patch https://github.com/RIOT-OS/RIOT/pull/8671.diff -- You are receiving this because you are subscribed to this thread. Reply to this email directly or view it on GitHub: https://github.com/RIOT-OS/RIOT/pull/8671 -------------- next part -------------- An HTML attachment was scrubbed... URL: <http://lists.riot-os.org/pipermail/notifications/attachments/20180228/98beb8ea/attachment.html>


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Lindsey H. Emerson
Follow Lindsey on LinkedIn
J.D., University of Mississippi School of Law – May 2016
B.A. in History, University of Arkansas – May 2013
B.A. in Political Science, University of Arkansas – May 2013
Legal Experience
Law Clerk, Office of the Attorney General State of Mississippi – July – Aug. 2014
Legal Extern, U.S. Bankruptcy Court, Eastern & Western Dists. of Ark. – May – June 2014
Areas of Interest
General Litigation
Bankruptcy Law
Corporate Law
Criminal Law
Professional Associations & Memberships
Staff Editor, Mississippi Law Journal – Volume 84
Member, Law Association for Women – 2L, 1L
Member, Public Interest Law Foundation – 2L, 1L
Member, Phi Delta Phi – Legal Honor Society – 2L, 1L
Member, Law School Student Body Association – 2L, 1L


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Preliminary Accident Reports
May 2000 Issue
Feb. 8, Alma, Mich. / Piper Tomahawk
At 07:30 EST, a Piper PA-38-112 lost power near Alma and was damaged in the ensuing forced landing. The pilot reported minor injuries. The airplane had departed the Chippewa County International Airport at about 05:05 and was en route to Alma. The pilot said he had flown from Alma to Chippewa County the previous day and did not refuel the aircraft prior to the accident flight. The pilot admitted to investigators that he did not calculate total fuel consumption for the trip. The pilot made a forced landing in a snow-covered field and the airplane nosed over after striking a fence line. Examination of the aircraft revealed no preexisting anomalies with the aircraft. There was no evidence of fu...
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DGio The Artist - Sundress Season
Check out Sundress Season by DGio The Artist on Artist Sounds! DGio The Artist is a Texan rapper. He puts his heart and soul into his music, giving a voice to the millennial generation. DGio's work reflects all the travelling he did in his childhood, and combines haunting production with DGio's unique style of rap. His latest track, Sundress Season, is an ode to all the beautiful women rocking their sundresses this summer. The track is a perfect summer hit, typical of DGio's music. Give it a listen on SoundCloud and keep an eye out for more of DGio The Artist's work.