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Big and loud: My Favorite Writers by Frank De Blase
The other night I had the opportunity to speak as a special guest at a ladies monthly book club meeting. You know the deal; they all read the same book then get together to discuss its finer points. This month's selection was my debut novel for Down & Out Books, PINE BOX FOR A PIN-UP.
OK, look, I know what you're thinking: a suburban hen party, plenty of merlot, and healthy snacks that no one touches as long as the chocolate/peanut clusters hold out, where they gossip about whoever didn't show up and punctuate it all here and there with discussion of 50 shades of some Agatha Higgins cozy yawn.
I was flattered yet wary and somewhat hesitant. I'm a dark writer and though I adore women immensely, in my writing they are the source —and the victims — of much of this darkness. In many cases if a woman shows up in one of my stories, it's either as a painstakingly described femme fatale or dead. They are all to be admired, even if they make the scene as a corpse. There's some perverse value in being deemed killable and I wasn't in the mood to debate misogyny or exploitation or "What the fuck is wrong with you?" with some chick who thinks I'm a creep.
I couldn't have been more wrong. They were full of questions; my process, where did I get my ideas? Did I know how it was all going to end when I started? Would I like some more chocolate/peanut clusters? And the general what for, why is, and when by.
But it was the question of influence that rendered an epiphany. And as I began to explain, it became increasingly clear to me. Sure, I got turned on to the crime/noir genre by Spillane, Chandler, Prather, Hammett, Thompson, Goodis, Slade, Ellroy, Edgerton, Mosley, and so on. But I've been an outsider in every artistic endeavor I've ever pursued. Why should writing be any different?
Photography sprang from music and writing sprung from both. Though there are procedures and rules and technical knowhow required, I always drove around all that silly stuff and did it wrong. In other words: my way. I applied what I knew of other artistic expression to the art at hand and it has served me well. My pictures look like my writing and my writing sounds like my music.
But I'm getting off track, here. We were talking about influential writers and mine, I discovered, aren't really the masters of the printed page you would think. No dark, brooding chain-smoking types haunted by the characters they create as they peck away at their own demons on an old Smith-Corona and drown in booze, no. One slinked around the stage like a panther with a loud guitar, the other celebrated in film, women with breasts the size of Volkswagens. Yup, the two biggest influences on my writing are guitarist Link Wray and filmmaker Russ Meyer.
You can talk about early rock 'n' roll, how its savage beat was going to warp our youth, rape our women, and allow America to fall into the hands of the Reds. Or rant about either its nonsensical rama lama ding dong-ing or double entendre, and Link Wray's 1958 hit single "Rumble" was banned because it was said to incite juvenile delinquents to riot. But "Rumble" was an instrumental — no lyrics, jack — just the swaggering throb of three chords pounded out with a clenched fist.
Link's primal rhythms echo a femme fatale's strut: swivelin,' swingin,' sexy and capable of causing a riot. His guitar is the sound of the getaway car, the five o'clock shadow on a killer's face, it's bullets ricocheting down the alley.
Mr. Nice Guy spit blood and cursed under his breath. Propping himself up, he wiped his mouth. He wasn't used to being at the wrong end of a gun…his gun.
"Take it easy, pal," he said to the little man with the big gun. "Let's not get excited. Let's not do anything we'll regret."
"You were here to k-k-kill me," the little man stammered. Mr. Nice Guy had to think fast.
"You've got it all wrong, pal," he said. "I didn't come here to kill you. That's all big talk to keep up appearances for the real deadbeats. Look, you're a pro, everyone knows that. But I gotta come on hard, or the fresh fish will take advantage. I came here to work it out, pal. I wasn't here to kill you."
Mr. Nice Guy was there to kill him. The little man with the big gun was a skell on the skids, a degenerate gambler who owed the wrong people a lot of money; people Mr. Nice Guy worked for. His account was past due being past due. He was out of strikes, he was maxed out. An example needed to be made.
— From the story "Big Man, Little Gun" included in the short story compilation BUSTED VALENTINES AND OTHER DARK DELIGHTS by Frank De Blase
Though couched in social commentary, Russ Meyer's name is synonymous with the Double D cup and the often violent, sexified sexpots that routinely spill out of it.
He frequently filmed and photographed these top-heavy, cantilevered cuties from low angles to accentuate their dominance and celebrate — and accentuate — the gonzo, jaw-dropping busty-ness of it all. There are obvious literal references to Meyer when I describe the desired female victim, villain, or vixen. And like Meyer, I try to balance objectification and worship as both are desirable and dangerous, perfect for setting up the male character who can't tell the difference.
It was early evening when I set out to look for Mickey. He wasn't hard to find. He hung out at The Bamboo Club on East Avenue. And it was easy to see why. The Bamboo Club was a tiny burlesque joint—about three times the size of a phone booth—catering to folks who liked to tie one on as the girls took it off, bump'n'grind with a twist of lime.
It was intimate, as the crowd was shoehorned in elbow-to-elbow to take in a striptease act that was practically in their laps, given the establishment's diminutive layout. The gals at The Bamboo Club all had maximum peel and squeal appeal, with torrid displays of agility and anatomy that periodically had owner Hy Isaacs in court explaining this was art and the girl in question was convent-bred and taking care of her sickly mother.
I strolled in as a large breasted and rather callipygian dancer on sparkly heels was in the final stretch of her routine. She was what some might call plump, but something I just considered busty all over—built for comfort, my kind of gal.
The strip portion was over and now it was all about the shimmy and shake. This gal was wholeheartedly shaking her backside into a delicious blur. Around and around it went just like the mesmerized eyes of the guys in front. — From the novel PINE BOX FOR A PIN-UP by Frank De Blase
When I sit down to write, these are the tools I apply to a story: the D chord and the D cup, the ferocious kerrang of the guitar, the mouthwatering allure of generous pulchritude. Both celebrate extremes, both inspire bravado, and fear. It's just big and loud and undeniably cool. And frankly, who wouldn't want to read that?

subset: commoncrawl


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In a small living room in Ridgewood, Queens, Patricia Vesantes, a 50-year-old woman from Cuenca, Ecuador, called her sister, Rosa Vesantes, to sit on her brown couch.
"I have never heard of a worker cooperative," said Rosa in Spanish. Twelve years ago Rosa immigrated to New York City from Cuenca, Ecuador and works to support herself, husband, and three daughters by selling homemade clothing and party decorations. The Queens apartment is reasonably sized by New York standards and furnished with leather furniture against beige colored walls. A striking contrast to the vibrant hues that decorate the streets of Vesante's hometown. In the center of the living room is a wooden table with a sewing machine on top. A few strips of red, purple, and pink cloth dangled across the table's edge. Vesantes' business is run from her own living room; in an apartment on the floor below her sister's. Rosa's youngest daughter, who is 11 years old, lives with her in Ridgewood. But her 14-year-old and 15-year-old daughters still live in Cuenca, due to financial reasons.
"It is hard not having them here," said Vesantes. Vesantes said that she wishes that her whole family could be together in New York but that most likely that will not happen anytime soon.
"I send money back to my family in Ecuador so that they can get things for my daughters," said Vesantes. She said that she tries her best to be a part of their lives and she hopes to be able to bring them to New York in time for them to attend college.
Vesantes left her minimum-wage job at a daycare when she received financial help to pursue her home-based entrepreneurial venture. Her niece who worked for Grameen America, a nonprofit microfinance organization based in New York City, told Vesantes that she could qualify for a loan from the organization. Grameen America specifically lends to female immigrant entrepreneurs who live below the poverty line.
In order for Vesantes to receive her loan, she first had to find four other immigrant women interested in starting their own businesses. She found four people within a week. Vesantes then, along with the other recruited women, attended a week-long financial training boot camp and were educated about loans, building credit, and savings. At the end of the week, each woman opened a savings account and was given a $1,500 loan said Vesantes. After receiving the loan Vesantes has to meet every week with her loan counselor to help her stay on track.
"I wouldn't be able to have my business without it," said Vesantes. "I get the loan a couple of times a year."
New York City spent $606.7 million this year in Career Pathways, a national workforce development strategy designed to increase education, training, and learning opportunities for the current and emerging workforce of men and women. The city has allotted $5.7 million of the $606.7 million for entrepreneurship training, according to the 2016 NYC Career Pathways Update Report.
Of those $5.7 million, $2.1 million was distributed to worker cooperatives by the New York City Council to the Worker Cooperative Business Development Initiative and the Small Business Bureau, according to the 2016 report from the SBS on the WCDBI. According to Christian Gonzalez-Rivera, a researcher at the Center for an Urban Future, this is not enough money to help new worker cooperatives to improve and grow. At this rate, worker cooperatives will resort to transforming into more traditional companies to survive and cease to be worker cooperatives.
A worker cooperative is a business entity that is worker-owned and worker-controlled. Usually, workers invest in a buy-in amount when they join the cooperative and then begin working. At the end of each year a worker also referred to as a worker-member, is paid a portion of the business's profit. This money, which in the co-operative world is called a surplus, can be distributed by seniority, hours worked, or other criteria.The first worker cooperative was started in Manchester during the Industrial Revolution as part of the cooperative movement sparked by the Welsh socialist, Robert Owen. During the 19th there was a movement to transform factories and business into worker-cooperatives. But it was largely unsuccessful. A rebirth of interest in worker cooperatives started in 1960's.The benefit of a worker cooperative is the sense of agency and community workers have, but they are difficult to grow past a certain number and the larger the cooperative the more difficult it to sustain.
Worker cooperatives abide by the rule of "one worker, one vote," according to Adria Powell, Executive Vice President and President-Elect of Cooperative Home Care Associates. This Bronx-based cooperative is the largest worker-owned business in the nation with 2,300 worker-owners with 90% being women of color.
The "one worker, one vote" mentality means that decisions within the worker cooperative are made democratically by voting. Any business can theoretically become a worker cooperative and there are cooperatives in a number of different sectors, such as service, retail, healthcare, skilled trade, manufacturing, technology, education, media, and arts. JustSeeds is a street art worker cooperative based in Pittsburgh, Tech Co-op Network is a North American tech-worker cooperative, and the 3B Brooklyn is a bed and breakfast that is also a worker cooperative.
According to the report and Nick Benson, the press secretary of the New York City Department of Small Business Service, a group that helps business in the city start, grow and operate, 27 worker cooperatives were started in 2016 compared to a handful of worker cooperatives in years past. The initiative worked on outreach to small business across the city and spread information about the worker cooperative model by having people visit business and provide educational pamphlets about worker cooperatives. It claims that 2164 entrepreneurs have been reached in 2016. A slight increase in the number reached in 2015. This number is the sum of individuals who engaged with worker cooperatives through in-person activities like lectures, group training, conferences, and one-on-one assistance.
The WCBDI highlights its efforts in business incubation, stating that its "Business incubation services are hands-on and customized to meet the needs of each unique set of entrepreneurs." Its partner organizations try to equip potential worker cooperatives with the knowledge of how to run a worker cooperative. They also provide them with basic skills need to launch and grow a business. The WCBDI did not list those skills.
The WCBDI also provides financing assistance, legal assistance, and business development. Business development services fall into 8 categories: business plan development, marketing and market research, governance and internal manuals assistance, strategic planning, translation services, bookkeeping, financial planning, and succession planning.
But having initiative volunteers go door-to-door to deliver pamphlets and give a quick summary of the worker-cooperative model was not very effective in converting more businesses to worker-cooperatives. None of the women that joined Damayan, a migrant workers association, within the last year were educated by the initiative about worker cooperatives.
New York City hosts the largest number of women-owned businesses in the country. According to a report from the Center for an Urban Future, the city was home to 413,899 women-owned business in 2012.
The number of women deciding to become entrepreneurs is barely increasing and the start-up gap between the genders is still large. The Kauffman Foundation states that the number of male entrepreneurs in 2016 was 0.42 % compared to the number of females which is 0.26%. Immigrants are more likely than native-born to be entrepreneurs but they face a list of challenges that the native-born do not.
According to a study conducted by the Ewing Marion Kauffman Foundation, a nonprofit that focuses on giving grants to young entrepreneurs, 2016 has seen the largest increase in the number of female entrepreneurs in last 20 years. The rate of new female entrepreneurs has gone up from 0.22 % to 0.26 %. This means that out of 100,000 all women 260 become entrepreneurs in a given month in 2016.
Immigrant entrepreneurs now makeup 27.5 % of all entrepreneurs in the United States, male and female, and are more likely than native-born Americans to become entrepreneurs. Out of 100,000 immigrants, 530 will become entrepreneurs a large difference from the 290 out of 100,000 native-born that will become entrepreneurs in a given month (for the year 2016) according to the same report.
Daniella De Santos came to New York on a work visa in 2001. In her home state of Bahia in Brazil she had met an American woman that was interested in opening a salon in New York City. But she needed someone who could bring something new and exotic to her business. De Santos' specialty was Brazilian bikini waxing. " Nobody knew how to do it in the United States at the time," said De Santos. The American woman hired De Santos on the spot. De Santos worked for her at Sony Skin Care for six years before opening her own business.
"I did my business plan, found a place to rent, and bought my equipment," said Daniella de Santos, owner of Ipanema Spa, Ms. De Santos opened her business in March of 2007.
De Santo's business, Ipanema Spa, sits above a Manhattan pizzeria. The inside is decorated with short dark colored candles around piles of white towels.
"Coming from a third world country you know things are not easy, so I knew it was not going to be easy," said De Santos in reference to starting Ipanema Spa. She had fears that the clientele she had acquired working at Sony Skin Care might not follow her to her new location. "No matter what it would be a challenge."
Ms. De Santos is proud that her spa has stayed in business for nine years. In recent years she has faced hardship due to a lack of well competent employees who want to commit to working at Ipanema for a long period of time.
De Santos said that it was difficult going out on her own to start her business and wished that she would have had help like a network of some kind of other immigrant women that started their own businesses.
As female immigrants, Rosa Vesantes and Daniella de Santos said they would benefit from being part of worker cooperatives.
Immigrant entrepreneurs are frequently less likely than native-born entrepreneurs to have access to capital due to being and are less financially literate. These are due to a lack of established credit history in the U.S and in the case of some immigrant communities a lack of access to basic banking and financial services, payday loans, non-risky mortgages. Immigrants are also more likely to fall prey to poor financial products.Immigrant entrepreneurs will often not have the necessary collateral to secure a loan. According to the Brookings Institute and the Federal Reserve Bank of Chicago, only 63% of immigrant head of households have a checking account, compared with 76% of native-born head of households have a checking account at a brick and mortar bank.
Immigrant entrepreneurs may also have a hard time navigating the business world's regulatory environment. According to the New York City government website, In New York City alone there are more than 6,000 business-related rules and regulations. There are 250 licenses and permit requirements for business. Navigating the complex process can be made even more difficult due to language barriers and cultural differences.
Within some immigrant communities there is also a level of distrust of the government.
A city policy organization called The Center for an Urban Future lies within a building with a warmly lit lobby. After passing through security and ascending in the elevator, mid-way up the building is home to the actual offices, which are austere and dim.
At the end of The Center for an Urban Future's main hallway is where Mr. Christian González-Rivera's office resides.
"Right now we think small but we need to think big," said González. Mr. González-Rivera said that worker cooperatives are effective because they give more agency to individuals that might not have had as much power in a more traditional work environment.He said worker cooperatives are particularly beneficial to immigrant women that
His major concern is the lack of business incubation and business development efforts. Worker cooperatives lack a mentoring culture and are difficult to grow past a few dozen worker-members. " We need to be able to teach worker-owners who are part of worker cooperative how to be in a worker cooperative," said González-Rivera. According to Gonzalez-Rivera, there is poor communication in worker cooperatives and the problem only gets worse the larger the worker cooperative. There needs to be a larger scale education on the worker-cooperative model because since the idea is foreign in the United States said Gonzalez-Rivera. If communication within smaller worker cooperatives is perfected than it can be improved in larger cooperatives. The key to preventing a worker cooperative from being a traditional business is communication and having all worker-members be heard said Gonzalez-Rivera.
Adria Powell remembers how she used to spend time with her mother at Cooperative Home Care Associates. She filed and worked as a receptionist and when she came home from college in 1993. " I started working for the summer as the operation manage," said Adria Powell. "At the end of the summer she asked me if I wanted to stay on whole time."
Ms. Powell has always loved her work since she believes that the populations being served by a worker cooperative, meaning the worker-owners, have faced social injustice.
"Worker cooperatives provide a type of work environment that promotes equality, inclusion, and agency," she said.
According to Adria Powell what made CHCA special was the close-knit community. Everyone felt as if they were part of a large family. Now it has over 2,000 worker-members.
According to Ms. Powell, who will be starting at the helm of the organization in January, CHCA will continue to grow.
This is the largest challenge of having a worker cooperative the size of CHCA. Powell admits that it is a struggle to have effective communication between so many people due to the difficulties of actually reaching out to so many individuals and inevitable miscommunications.
Everyone at Cooperative Home Care Associates had a say in the decision-making. But worker-member numbers started to creep over 400 in 2003 the organization became unionized but is still technically a worker cooperative. Powell recalled that when decisions had to be made a large box was passed around the company to collect paper ballots from members.
The cooperative would vote on wage increases, health benefits, such as if they wanted to add vision or dental to their health coverage. It would vote on the distribution of profits if the company should start a contract or end a contract if it would have distribution on 401k plan, said Powell.
"But almost all was taken out of our hands when we were unionized," she said.
Now CHCA worker-members vote to elect eight individuals positions in a collective bargaining unit that then makes decisions. According to Powell there is an open door policy and may come and go as they please in the organization as long as they pay dues and vote.
"There is no money that we would be getting from that pool," said Powell.
The money allotted by the city of New York to help worker cooperatives $2.1 million but CHCA does not receive any benefit from it. It is too large of an entity and the city council's money is meant for smaller worker cooperatives that perhaps have not been unionized.
Cooperative Home Care Associates has provided thousands of jobs to women and female immigrants in the Bronx but it is straddling the line of a true cooperative and traditional cooperation. It too needs help staying true to its cooperative roots but is not getting the help it needs.
"I would love to see more money invested in worker cooperatives in the model because it is beneficial to people that are marginalized. but I don't know if that is a reality," said Powell. She too realizes that there is a large number of untouched talent that have never heard of worker cooperatives. Women like Rosa Vesantes and Daniella De Santos who did not hear about worker cooperatives in time to make their lives easier. "So we start small educating various constituents about the worker cooperative, so it doesn't hurt us to have new coops started but there needs to be a strategy for sharing resources."
Rosa Vesantes leaned back on the brown sofa and looked at her sister Patricia. "That sounds good," she said in reference to worker cooperatives. " If it really is something that helps it's workers get better pay and have better jobs I would have liked to learn more."


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Gus Allbritton, a volunteer for the Carl Vinson V.A. Medical Center in Dublin says care at that hospital remains strong despite problems at other locations across the country.
Recently, the Department of Veterans Affairs has faced allegations that 40 people may have died at a V.A. center in Phoenix after being delayed care.
A veteran told WXIA in Atlanta that he has waited months for treatment at a facility in Cobb County.
Many parents and educators across the U.S. are coming to realize the benefits to students of Olympic Sport Fencing. Sport fencing has been a small but ever present sport in America since the 19th century. Usually, and erroneously, associated with the privileged class, it has found little place at large in the ball dominate sports arena in America. However, that view has been changing rapidly over the 25 years. The U.S. has grown steadily in world prominence including generation of World Champions and Olympic Gold and Silver medalist. This growth is attributed with broader media coverage but also with a concerted effort by coaches and fencing enthusiast to promote this exciting sport at the middle school and high school levels around the country. This moves fencing out from the traditional private salles (clubs) and college environments so as to make it accessible to everyone who is interested.
See how Houston County restaurants rated with the Georgia Department of Health from March 25-April 25:
Defense Secretary Chuck Hagel said Monday that he supports a force-wide review of tobacco use and sales on military installations.
"I think we owe it to our people," Hagel said at a Pentagon news conference.
The military's health care costs attributable to tobacco use are "astounding, well over a billion dollars" a year, Hagel said.
"Dollars are one thing, but the health of your people, I don't know if you put a price on that. So I do think it needs to be looked at and reviewed," Hagel said.
"Whether we, in the military, through commissaries [and] PXs, sell or continue to sell tobacco is something that we need to look at. And we are looking at it," Hagel said.
The TRICARE benefits walk-in service center at Robins Air Force Base is closing Tuesday, the base says.
TRICARE is the Defense Department health care program. According to its website,Tricare covers about 9.6 million active- duty service members, National Guard and Reserve members, retirees, their families and survivors.
The Department of Defense is closing all of its service centers nationwide to save an estimated $250 million over the next five years.
They say the centers are becoming obsolete due to advancements in on-line technology that lets people manage benefits from home. Walk-in centers are the most expensive service option, the Department of Defense says.
ROBINS AIR FORCE BASE, Ga. – Medical patients here looking for pain relief may soon have the option of nontraditional treatment.
The treatment, called battlefield acupuncture, was recently introduced to physicians and other healthcare professionals by Dr Tom Piazza, one of three acupuncturists at Andrews Air Force Base, Md.
Piazza led a day-long training session at the 78th Medical Group to get personnel acquainted with and ultimately credentialed on the battlefield acupuncture. Students received hands-on instruction by performing the acupuncture procedure on each other as well as volunteers seeking relief from persistent pain.
The procedure is specific to the surface of the ear and consists of five small needles, which remain in place for two to four days before being removed or falling out on their own. Though performed in minutes, results of the procedure are often felt within seconds.
Bruce Horovitz, USA TODAY
Burger King is tweaking its Kid's Meals with fries that are less nutritionally naughty.
The burger giant Tuesday announced that its crinkle-cut Satisfries -- with 40% less fat and 30% fewer calories than McDonald's fries -- will immediately become the standard fries served in Burger King Kid's Meals nationally. Although BK charges slightly more for Satisfries with adult meals, it will not charge more for its Kid's Meals.
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My Advocare Health and Wellness Challenge – Day 19
Loose Fittin' Jeans
Yesterday marked the end of day 19 of the 24-Day Challenge. We are really racing to the finish now. Obviously I'm not posting every day, since there hasn't been too much to share. One thing I did notice yesterday: I had to pull my jeans up. A lot. Isn't that fun?
I was also reminded how truly 'craving-free' I have been for quite some time. If you have been reading along from the beginning, you may remember that it was around day 3 or 4 that it seemed like my body began to fight back with cravings. It was as if my stomach was saying, "Wait a second…this isn't a mistake. He's cutting out these things on purpose!" But a couple of days later, the cravings were gone. For good. All it took was a little determination and some intentional planning. Now the challenge is almost over.
That's enough for this morning. Looking forward to a great day. I hope you are as well!Image Source: http://grover.net


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- Research
- Open Access
Searching for bidirectional promoters in Arabidopsis thaliana
© Wang et al; licensee BioMed Central Ltd. 2009
- Published: 30 January 2009
A "bidirectional gene pair" is defined as two adjacent genes which are located on opposite strands of DNA with transcription start sites (TSSs) not more than 1000 base pairs apart and the intergenic region between two TSSs is commonly designated as a putative "bidirectional promoter". Individual examples of bidirectional gene pairs have been reported for years, as well as a few genome-wide analyses have been studied in mammalian and human genomes. However, no genome-wide analysis of bidirectional genes for plants has been done. Furthermore, the exact mechanism of this gene organization is still less understood.
We conducted comprehensive analysis of bidirectional gene pairs through the whole Arabidopsis thaliana genome and identified 2471 bidirectional gene pairs. The analysis shows that bidirectional genes are often coexpressed and tend to be involved in the same biological function. Furthermore, bidirectional gene pairs associated with similar functions seem to have stronger expression correlation. We pay more attention to the regulatory analysis on the intergenic regions between bidirectional genes. Using a hierarchical stochastic language model (HSL) (which is developed by ourselves), we can identify intergenic regions enriched of regulatory elements which are essential for the initiation of transcription. Finally, we picked 27 functionally associated bidirectional gene pairs with their intergenic regions enriched of regulatory elements and hypothesized them to be regulated by bidirectional promoters, some of which have the same orthologs in ancient organisms. More than half of these bidirectional gene pairs are further supported by sharing similar functional categories as these of handful experimental verified bidirectional genes.
Bidirectional gene pairs are concluded also prevalent in plant genome. Promoter analyses of the intergenic regions between bidirectional genes could be a new way to study the bidirectional gene structure, which may provide a important clue for further analysis. Such a method could be applied to other genomes.
- Intergenic Region
- Gene Pair
- Thymidine Kinase
- Functional Similarity
- Random Pair
In our study, we pay more attention to the regulatory analysis of the intergenic region between bidirectional genes. In the papers mentioned above, a "bidirectional promoter" is presumedly defined as the intergenic DNA sequence between two TSSs of a bidirectional gene pair. However, increasing evidences show that in eukaryotic organisms, promoters are not always in the vicinity of TSSs of genes, but can locate from the distant 5' upstream regions of genes, or to the 3' downstream regions [9, 10]. Recently the ENCODE pilot project even suggested that the human genome is pervasively transcribed [11] and it is noted that most genes are controlled cooperatively by several transcription factors (TFs) binding to various regulatory elements [12, 13] which are often located spatially close to each other. Therefore, in the scrutiny of intergenic DNA sequences between bidirectional genes, an additional criterion was used here to see whether those sequences are also enriched of regulatory elements which are essential for the initiation of transcription. The hierarchical stochastic language (HSL) model we recently developed [14] were utilized to identify putative transcriptional regulatory regions (TRRs) on those intergenic sequences between bidirectional genes by scoring them. The TRR regions identified by HSL are enriched of cooperative motif pairs which are over-represented in promoters. We found that these cooperative motif pairs selected from yeast are conserved across species and HSL trained on yeast are successfully applied to identify promoters (functional regions) for fly, human, Arabidopsis thaliana, and rice [14].
In this article, we conducted a genome-wide survey of Arabidopsis thaliana genome and identified 2471 bidirectional gene pairs in the whole genome (excluding chloroplast genome and mitochondrial genome). Besides examining the expression and function association of bidirectional genes as previous studies [2, 7], we searched the intergenic DNA sequences between bidirectional genes by HSL to see if they contain TRR. We found that these 2471 bidirectional gene pairs show a higher probability to be coordinately regulated and functionally associated which are consistent with results in mammalian [2]; and thus indicate that this "head-to-head" gene organization is prevalent in A. thaliana genome. We further showed that functionally associated bidirectional genes have higher frequencies to contain intergenic sequences with higher TRR scores, indicating that these sequences response for co-regulating the bidirectional genes. Interestingly, most of the intergenic DNA sequences of bidirectional genes with high TRR scores contain only one peak of TRR score, suggesting that the gene pair share the same promoter. Finally, we picked 27 functionally associated bidirectional gene pairs with high TRR scores and hypothesized them to be regulated by bidirectional promoters. Five of them share common ancient orthologs and in addition, more than half of them are further supported by sharing the same functional categories (GO terms) as these of handful experimental verified bidirectional genes in other organisms.
Identify bidirectional genes
Coexpression of bidirectional genes
Functional similarity of bidirectional genes
In the previous studies about human genome, researchers noted that many bidirectional transcribed genes are related to DNA-repair mechanism [1, 2]. It means that head-to-head structured genes may be associated functionally. To test this hypothesis, we analyzed the functional similarity of bidirectional genes using the Gene Ontology (GO) functional annotation (See Method for details). GO is a rooted directed acyclic graph (DAG) with three subcategories of "cellular component", "molecular function" and "biological process". Each GO node covers a set of genes with specific annotations and a gene is covered by one or multiple GO nodes along the DAG generally. The closer a node is to the terminal, the more detailed annotations are given. Similarly to Deng et al [19], those nodes that cover at least 50 genes and none of their offspring node satisfying this condition are defined as terminal informative nodes (TINs). We obtained 31 TINs in subcategory "cellular component", 82 TINs in "molecular function" and 125 TINs in "biological process" respectively, which can be found in the Additional file 1. Among all the 37019 genes of Arabidopsis thaliana, 17365 are covered by at least one TIN, namely 2898 genes with "cellular component" TINs, 11140 with "molecular function" TINs and 10242 with "biological process" TINs, respectively. We declared a pair is annotated by meaning that both genes of the pair are covered by at least one TIN in a specific subcategory and assigned functional similarity as 1 if the two genes have overlapping TINs and 0 otherwise. Among all the 327 annotated bidirectional gene pairs, we found 34 pairs (10.4%) of them having functional similarity (functional similarity is 1) and 293 pairs having no functional similarity (functional similarity is 0), whereas the counterpart number in the 10000 random pairs are 109 (6.21%) and 1645. Such a difference is significant with a p value 1.33 × 10-3 if we use a binomial background distribution, which supports the hypothesis that bidirectional genes are more likely to be functionally associated than randomly paired genes.
Coexpression levels on groups with different functional similarity
Coexpression levels on different groups.
2471 bidirectional gene pairs
Fun_sim = 1
Fun_sim = 0
Identification of transcriptional regulatory region (TRR)
In previous studies, putative bidirectional promoters are defined as intergenic regions between TSSs with not more than 1000 base pairs long. However, a region designated like that may not contain functional promoters since promoters can locate faraway from TSSs, and the two genes may locate "head-to-head" closely along genome by a chance. Beware of this and because of the difficulties for experimental verifications of promoters, we further scrutinize the DNA sequences between the bidirectional genes by HSL we recently developed to see if these sequences contain potential functional regulatory regions (See Methods for details).
Functional similarity on high scoring TRR group and low scoring TRR group
Functional similarity on different groups.
Fun_sim = 1
Fun_sim = 0
High scoring group
Low scoring group
Random pairs
Predicting potential functional bidirectional promoters
True bidirectional promoters we predict.
GO term
Function description
ATP binding transmembrane receptor activity
ATP binding transmembrane receptor activity
ATP binding transmembrane receptor activity
ATP binding transmembrane receptor activity
ATP binding transmembrane receptor activity
protein amino acid phosphorylation
protein amino acid phosphorylation
protein amino acid phosphorylation
protein serine/threonine/tyrosine kinase activity protein amino acid phosphorylation
protein amino acid phosphorylation
anchored to membrane
lipid binding anchored to membrane lipid transport
transcription factor activity
transcription factor activity
response to auxin stimulus
response to auxin stimulus
zinc ion binding
zinc ion binding
structural constituent of ribosome
electron carrier activity
translation initiation factor activity
response to heat
thiolester hydrolase activity ubiquitin-specific protease activity
ubiquitin-dependent protein catabolic process
RNA binding
monovalent cation: proton antiporter activity
defense response to bacterium
Among these 27 gene pairs, we found for each of five pairs (pair2, 4, 6, 7 and 26), the bidirectional genes contain the same functional protein domains and also share same orthologs from Nematode, Yeast and Fungi, which are evolutionally more ancient than A. thaliana (See Additional file 2 for details). The results indicate that genes in each of these five pairs are derived from a common ancestor into two genes during evolution.
Of the 27 bidirectional gene pairs, five pairs (pair 1–5 in Table 3 – True bidirectional promoters we predict.) sharing the same two GO terms GO:0005524 and GO:0004888, five pairs (pair 6–10) sharing the same GO term GO:0006468, two pairs (pair 11–12) sharing GO:0031225, two pairs (pair 13–14) sharing GO:0003700, two pairs (pair 15–16) sharing GO:0009733 and two pairs (pair 17–18) sharing GO:0008270. There are 20 GO terms being shared by identified bidirectional promoters in total, and most of these GO terms are supported by previous studies. Guarcuaglini et al found that murine RanBP1 and Htf9-c genes is regulated from a shared bidirectional promoter during cell cycle progression [20]. The Mouse Genome Informatics (MGI) database annotates Htf9-c with GO: 0003723 (RNA binding) which is listed in Table 3 – True bidirectional promoters we predict. [21]. Thymidine kinase (TK) is a growth factor inducible enzyme that is highly expressed in proliferating mammalian cells. Expression of mouse TK mRNA is controlled by transcriptional and posttranscriptional mechanisms including antisense transcription. Schuettengruber et al had identified a novel gene which is divergently transcribed from the bidirectional TK promoter, encoding kynenurine formamidase (KF), an enzyme of the tryptophan metabolism [5]. Murine gene TK is annotated in GO:0005524 (ATP binding) which is also in our prediction list by MGI annotation. Our prediction can also be supported by evidences in human genome. Xu et al concluded that the expression of human genes NBR2 and BRCA1 are co-ordinated through a bidirectional promoter [22], and Wu et al confirmed that BRCA1 have function GO:0008270 (zinc ion binding) which is shared by pair 17 and 18 [23]. Human genes PRKDC and MCM4 are divergently transcribed and located at chromosome 8 band q11 [24]. Gene PRKDC is annotated in GO:0018105 (peptidyl-serine phosphorylation) [25] which is a son node of GO:006468 (protein amino acid phosphorylation) in our prediction. Whereas MCM4 is covered by GO:0005524 (ATP binding) which is shared by pair 1–5 through the annotation of Gene Ontology. Besides that, Momota et al found that human genes COL4A3 and COL4A4 coding for the human alpha3(IV) and alpha4(IV) collagen chains are arranged head-to-head on chromosome 2q36 [26], while COL4A3 is annotated in GO:0006468 (protein amino acid phosphorylation) by Raya et al [27]. Bellizzi et al confirmed that two human genes SIRT3 and PSMD13 shared one bidirectional promoter [28], while gene PSMD13 is covered by GO:0031145 (anaphase-promoting complex-dependent proteasom-al ubiquitin-dependent protein catabolic process) through the annotation of Gene Ontology [29], which is a grandchildren of GO: 0006511 (ubiquitin-dependent protein catabolic process) we also predicted. Besides that, we find two genes from one bidirectional gene pair which is with lower TRR score not regulated by common bidirectional promoter. The two genes AT3G11450 and AT3G11460 constituting one bidirectional pair are located on chromosome 3 with a 773 base pairs long sequence separating them and the TRR score of this pair is 25.8, less than threshold 30. AT3G11450 is a heat shock gene [30], expression of which is regulated by heat stress transcriptional factors (HSFs), while AT3G11460 encodes pentatricopeptide repeat (PPR) protein. Geddy and Brown [31] revealed genes encoding PPR proteins are not conserved in location in Arabidopsis genomes and may be subject to diversifying selection. All these examples have been confirmed experimentally or by literature, providing a convincing support of our hypothesis. The five nodes GO:0006511, GO:0003723, GO:0008270, GO:000-6468 and GO:0005524 annotated most of genes mentioned above and also covered more than half of gene pairs we predicted in Table 3 – True bidirectional promoters we predict.
Bidirectional gene organization is reported existing in mammalian genomes by many literatures[1–3, 32, 33], but no genome-wide study of bidirectional genes for plant genome. We perform a systematic survey for the bidirectional genes in Arabidopsis thaliana and find this head-to-head gene structure is also very common, about 13.3% of the all the A. thaliana genes are arranged in such architectural features. Similarly to other studies [2, 6], bidirectional genes are more correlated in expression and associated in function. Furthermore we find that bidirectional gene pairs sharing similar functions exhibit stronger expression correlations.
In previous studies, the intergenic region between two transcription start sites (TSSs) of a bidirectional gene pair is commonly designated as a putative bidirectional promoter. However, it is not clear whether this region has regulatory activity, or enriched of regulatory elements which are essential for initiation of transcription. We scanned the intergenic sequences by HSL and assigned a TRR score of it to make sure it contains transcriptional regulatory regions or not. It's interesting that most of the intergenic regions of bidirectional genes with high TRR scores contain only one score peak, suggesting that the bidirectional genes share common promoter for transcription initiation. Statistical results showed that functionally associated bidirectional gene pairs with high TRR scores are probably regulated by common regulatory elements taking effects cooperatively.
If we restrict ourselves to the high scored TRRs with functional associated bidirectional genes, we obtain 27 gene pairs, 5 pairs of which may be involved in the same biological process and share common orthlogs from Nematode, Yeast and Fungi. Surprisingly, almost all of them limited to several GO functions such as ATP binding et al., and most of them are supported by previous studies.
Individual examples of divergently transcribed gene pairs have been found for years, most of which concern human genome [22, 24, 26, 34] or mammalian genome [35, 36], but corresponding results on plants are rarely reported. We conducted a systematic analysis of bidirectional gene pairs for Arabidopsis thaliana genome. We found that bidirectional gene pairs account for a large proportion (13.3%) of all the A. thaliana genes, confirming that this structure is also prevalent in plant genome. Similarly to other studies, we found that bidirectional gene pairs are more correlated in expression and associated functionally. Furthermore, we also perform systematic promoter activity analysis. We evaluated whether the putative bidirectional promoter regions are enriched of regulatory elements which are essential for promoter function and finally predicted 27 pairs of bidirectional genes which share common bidirectional promoters more probably. Five of them have the same functions for sharing common ancient orthologs, and in addition, more than half of them are further supported by sharing the same functional categories (GO terms) as these of handful experimental verified bidirectional genes in other organisms. These results significantly expanded the knowledge of "head-to-head" gene organization in plant genome and our work provides a new approach for further study of bidirectional genes in other genomes.
Identify bidirectional gene pairs and neighboring gene pairs
We obtained the information of annotated genes of Arabidopsis thaliana including loci, orientation of DNA chains and so on from TAIR [30]. There are 37019 genes in total and we defined the transcription start site coordinate and transcription end coordinate as 5' boundary and 3' boundary of a gene's locus. We then calculated the distances between 5' ends and 3' ends for genes in the same region no matter in forward chain or reverse. A gene pair that located in opposite strands and with the distance between their 5' ends not more than 1000 base pairs is defined as "bidirectional". We called a gene pair "convergent" if they located in opposite strands and with the distance between their 3' ends not more than 1000 bps, "codirectional" if they located in the same strand and with the distance between their 5' ends or 3' ends not more than 1000 bps. When studying coexpression, we refer the "convergent" genes and "codirectional" genes as neighboring gene pairs.
Calculation of correlation coefficients using microarray data
The AtGenExpression project [37] includes many microarray data for comparing Arabidopsis thaliana responses to the biotic and abiotic environment. We downloaded the microarray data of 46 groups from its database and then complied the data from the same group to a data set. We normalized the data by quantile normalization before calculating the Pearson correlation coefficients for three kinds of gene pairs (bidirectional pairs, neighboring pairs and random pairs) in each of the 46 data sets independently.
Download Gene Ontology data
We downloaded the three ontology files of subcategories "cellular component", "molecular function", "biological process" and the annotation file of Arabidopsis thaliana from Gene Ontology [38] on January 31, 2008. And using these files, we defined 31 terminal informative nodes (TINs) in subcategory "cellular component", 82 TINs in "molecular function" and 125 TINs in "biological process" respectively.
Calculate p value of expression correlation coefficients
Brief description of HSL algorithm
We presented a hierarchical stochastic language (HSL) algorithm for the detection of transcriptional regulatory regions (TRRs) in eukaryotes [14]. Since in higher eukaryotes the promoter region of a gene is not easy to determine most of the time, whereas in yeast it usually coincides with the immediate upstream region of the gene, the upstream regions of the genes of yeast were used as putative TRR sample sequences (positive samples) for training of the algorithm. In the training procedures of HSL, words of length six are counted in yeast putative TRR regions as well as in coding/randomized regions. Then their occurrence frequencies in both cases are compared, where a high frequency in the first and a low one in the latter case can now be used as an indicator for a TRR region. This first-order analysis is extended in such a way that now the frequencies of word pairs are compared and constructed with likelihood ratio scores. (In principle this can be further extended to triplets, but we showed that the analysis with pairs seems to be sufficiently accurate.)
In the predication procedures, for a given DNA sequence, sum of scores of appeared word pairs was given for each window of L base pairs along the sequence to indicate the likelihood of core TRR, where L can be chosen based on the required resolution (we used L = 300 in this study). A threshold can be given to distinguish the TRRs from non-TRRs. We found that HSL model trained based on yeast achieved comparable accuracy in predicting TRRs in other species, e.g., fruit fly, human, and rice, thus demonstrated HSL model can be used to accurately predict core TRRs of transcripts across species
This work is supported by the National Natural Science Foundation of China (No. 30570425), the National High Technology Research and Development of China (No. 2006AA02Z331, 2008AA02Z306), the National Key Basic Research Project of China (No. 2003CB715903), and the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry.
This article has been published as part of BMC Bioinformatics Volume 10 Supplement 1, 2009: Proceedings of The Seventh Asia Pacific Bioinformatics Conference (APBC) 2009. The full contents of the supplement are available online at http://www.biomedcentral.com/1471-2105/10?issue=S1
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This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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From the banks of the frozen Lake Erie in early May 1831, Lucy Mack Smith admonished her despondent fellow Saints. "Where is your faith in God?" she asked. "If I could make my voice to sound as loud as the trumpet of Michael the archangel I would declare the truth from land to land and from sea to sea."
At the Pulpit contains fifty-four discourses given by Latter-day Saint women throughout the history of The Church of Jesus Christ of Latter-day Saints. Like Lucy Mack Smith, these women drew on inspiration and experience to declare their understanding of eternal truths. This book illustrates the history of women's public preaching in the church, but its most important feature is the actual words of Mormon women. From the time of Emma Hale Smith's earliest exhortations at meetings of the Nauvoo Relief Society, Latter-day Saint women have been charged to instruct their families and neighbors, their congregations and Relief Societies, and other organizations. The talks featured in this volume show Mormon women doing the spiritual and intellectual work inherent in a life of Christian faith—seeking to do good works, understand the atonement of Jesus Christ, and strengthen their own faith and the faith of those around them. These women endeavored to live what they believed and to help their listeners do so as well.
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Funding for a major new Alberta Metis centre has been announced by the federal and Alberta governments and the Metis Nation of Alberta (MNA).
The funding will be used to create a new Metis cultural gathering centre at the Metis Crossing heritage site, located downstream from Edmonton on the North Saskatchewan River within the Victoria District National Historic Site. Metis Crossing encompasses 512 acres and features a historic village, restored homesteads and nature trails. It offers outdoor programming such as voyageur canoe trips, festivals and comfort camping in newly built Metis trappers tents.
The new cultural gathering centre will provide a welcoming indoor space for visitors to enjoy after outdoor activities. With meeting rooms, classrooms, exhibit and interpretive spaces, it will also enable Metis Crossing to offer year-round programming for the first time with experiences such as trapping, night sky-watching and snowshoeing.
"Métis Crossing is a place of pride where we not only share culture and tradition but offer an opportunity for others to learn and experience Métis culture," says MNA President Audrey Poitras. "Our long awaited Cultural Gathering Centre, that has been a dream of Alberta's Métis people for decades, is finally underway and will open up so many more future possibilities for visitors to enjoy in every season."
"Investing in cultural infrastructure plays an important role in developing dynamic communities, promoting tourism and preserving Canada's heritage, including the history and experiences of the Métis," says Amarjeet Sohi, the Edmonton MP who is also the federal minister of Infrastructure and Communities whose department is providing $3.5 million in funding for the project through the Major Infrastructure Component of its Building Canada Fund.
"The Government of Canada is proud to support the Métis Nation of Alberta in honouring this region's—and Canada's—diverse heritage and history by investing in the construction of a new cultural gathering centre for Métis Crossing," Sohi said. "The new centre will not only serve as a year-round tourist attraction, it will also act as a social gathering place for the community where residents and visitors can celebrate the unique traditions, art and culture of the Métis."
"Métis Crossing will contribute to the local and provincial economy while showcasing the history of a proud people – the Métis people of Alberta, "said Richard Feehan, Alberta Minister of Indigenous relations. "Through this initiative, visitors from across the province and the country will learn about the importance of the Métis to Alberta and Canada through several exhibits and historical village tours." The Government of Alberta is contributing $1 million and the Métis Nation of Alberta is providing the remaining project costs.
Métis Crossing is the first Métis cultural interpretive centre in Alberta. It mirrors Métis core values such as self-sufficiency, respect for Elders, encouraging youth participation and cultural pride. This site is one of many initiatives and projects the Métis Nation of Alberta supports to develop the socio-economic and cultural well-being of Métis people in Alberta.
Construction of the new cultural centre is scheduled to be completed in the late summer of 2019.
The Government of Canada will invest more than $180 billion over 12 years in public transit projects, green infrastructure, social infrastructure, trade and transportation routes, and Canada's rural and northern communities.
$25.3 billion of this funding will support social (including cultural and recreational) infrastructure in Canadian communities.
$4 billion of this funding will support infrastructure projects in Indigenous communities.


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Graduate Account Officer at Eben System
At Eben System, We believe every great business idea takes wings with the launch of a professionally designed web solution. That is why we utilize the Internet in redefining the way business is transacted. Since 2004, Eben System has been creating verifiable online presence which has become indispensable for every company - to lead its competition and to secure a dominant position in the industry. We are dedicated to providing you with exceptional customer service and solutions that are result oriented that ultimately will help you in achieving your goals and objectives.
Job Title: Account Officer
Location: Lagos
Job Description
Closing Date
25th September, 2016.
How to Apply
Interested and qualified candidates should forward copy of their CV's to: firstname.lastname@example.com
Job Title: Account Officer
Location: Lagos
Job Description
- Preparation, examination and analyzing of financial statements, responsible for record keeping, bank reconciliation statements
- Reviews, investigate, correct errors and inconsistencies in financial entries and related activities.
- Prepares asset, liability, and capital account entries by compiling and analyzing account information.
- Documents financial transactions by entering account information.
- Maintains accounting controls by preparing and recommending policies and procedures.
- Reconciles financial discrepancies by collecting and analyzing account information
- Ensure the prompt and accurate processing of income and expenditure transactions
- Ensure that the purchases and payments, and credit control systems are kept under regular review, and to recommend and implement improvements.
- Ensure that the organization's income is promptly collected, accounted for and secured.
- Ensure that supplier's accounts are settled in accordance with agreed terms.
- Ensure the correct and efficient operation of the Nominal Ledger, ensuring that control accounts are reconciled on daily, weekly and monthly basis.
- Minimum of HND in any related discipline.
- At least 1-year experience in a similar role, preferably in an environment with high transaction volumes and multiple operational locations.
- Sound knowledge of MS office tools and accounting applications such as with sage pastel is a must
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- Excellent organizational skills a good eye for details, determine priorities meet regular deadlines and take/follow up action.
- Must be highly reliable and should be able to work with minimum supervision.
- ICAN, ACA or related professional qualification will be an added advantage.
Closing Date
25th September, 2016.
How to Apply
Interested and qualified candidates should forward copy of their CV's to: firstname.lastname@example.com


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We provide free delivery on orders of £60 or more. Please Click here for more details.
Please note all deliveries to the Scottish Highlands, Islands and all Offshore Islands will be subject to surcharges. Prices will be given on application.
The courier used by Chayo Pet Food runs a 3 day delivery service (for orders processed before midday). If there is no-one at home to sign for the food it will not be left, but a card will be left offering a telephone number for the local depot so that the food can be collected.
If delivery instructions are given, i.e. leave in garage – the food will be left as per instructions. Please note that although we can arrange for our couriers to leave your goods when you are not at home, we cannot be held responsible for any goods that are stolen or damaged due to them being left.
Should any delivery not be delivered within 96 hours (4 days) please contact us on 0161 973 3207 so that a trace can be put onto the consignment. Any claims for non delivery must be made to us within 4 days after that we cannot make a claim against Business Post.
Damaged goods and Shortages on arrival must be noted on the courier delivery note and reported to us on the day of delivery so that we can make a claim with our courier. Call 0161 973 3207 or email firstname.lastname@example.com Failure to notify us within the stated time period will result in the loss of your right to reject the goods and your right to have the defects remedied subject to our absolute discretion. Your statutory rights are not affected.
Returns, Refund and Cancellation Policy.
If you wish to return any item please ensure that it is undamaged and that you retain all original packaging and labelling. It is essential that your order be returned in the original packaging together with a brief description of the nature of the complaint. All labels must still be in place. Any return not complying with these terms may be rejected. If you are entitled to a refund on damaged, faulty or incorrectly described goods the cost of the item(s) will be refunded to you in full and we will make arrangements to collect the item(s) from you on your next regular delivery day, or via courier. We will determine whether a refund is appropriate. We will not refund any items that have already been used in any way. Your statutory rights will not be affected. Please Call 0161 973 3207 or email firstname.lastname@example.com regarding any returns.


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Kansas - Point of Know Return Anniversary Tour Reviews
Michael Cookson from Nashville, Tennessee
45 years since its inception, and 40 years since the release of it's multi-platinum album, the current iteration of Kansas brought its A game to perform the Point of No Return album plus some on Saturday night at the Ryman. With 2 of the original members, and a cast of talented support, the performance was great. The encore Carry On is exactly the way I want to remember them, and far from being only Dust in the Wind.
DK from Chicago, Illinois
Nice venue, Bathrooms scarce on the 2nd level....Kansas did not meet expectations for an "Anniversary Tour" Everyone waiting for more songs that made them what they were, but under-delivered. Crowd lit up when they played the few Leftoverture tunes, but the rest of the night, we sat there waiting for more. Save your money and find a good tribute band.
Scott from Detroit, Michigan
What a shame. For a level set:I love my music loud. But levels were set so high there was considerable distortion. Ruined harmonies , and actually made it hard to differentiate instrumentals. They could have pulled someone off of Woodward avenue and put them on the sound board. Or maybe they did...
HIGGY from Des Moines, Iowa
I recall seeing the band back in the late 70s, God's not been paying that close of attention due to the ganja going around. Went to the show on a moment's notice very glad I did. probably one of the most awesome shows I've seen in a long time. Thanks for delivering Kansas.
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Forum: General Discussion
If you need extended support, contact our Support Team.
Forum Guidelines (*) The moderators hold the rights to move or modify posts in order to keep the discussions clear or to facilitate the search.
General Discussion
YouTube Music
Topic: YouTube Music
Inviato Wed 17 Oct 18 @ 9:55 pm
Inviato Wed 17 Oct 18 @ 11:20 pm
Inviato Wed 17 Oct 18 @ 11:34 pm
Inviato Tue 23 Oct 18 @ 6:32 am
VirtualDJ is considered to be DJ software (not home use) and playing through it would break the terms of service.
Sure this has been explained many times before.
Inviato Tue 23 Oct 18 @ 8:26 am
The music industry is helping us so spend a little and they will give you terrific value for money.
VJ Pro
Don't worry about them being video..if required they will play like a simple audio mp3.
Inviato Tue 23 Oct 18 @ 8:44 am


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Sponsored Listings:
Deborah K Lantz (Postmaster) email@example.com
20856 Criders Rd
Criders, VA 22820
Criders, VA 22820
The Criders Post Office is located in the state of Virginia within Rockingham County. This location serves 214 Criders residents with a median income of $26,500. It's estimated that approximately 342 packages pass through this post office each year.


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Proverbs 3:6 says" In all your ways acknowledge him, and he will make straight your paths.
We read this verse so often that we tend to not focus on the word "acknowledge". Acknowledging the Lord means you seek Him first in all things. The problem with us is that we don't seek the Lord until after the effect. Seeking Him first will save us allot of pain and heartache.
Being focus means eliminating stuff and junk out of your life that is keeping you from focusing on the Lord. We tend to have way too much meaningless things that are cluttering our lives that we tend to make them our priority, while our spiritual life is suffering.
Unfortunately, we only acknowledge the Lord when we have problems and the moment the problem has dissolved we are back on track to our bad behavior. But if we learn to disciple ourselves to stay focus we will find out just how quickly what's really important and what's not.
I know for myself I have a busy life from teaching to working outside my home. It's not always easy staying focus because things do come up in our lives. When this happens and it does I take a step back take a deep breath and regroup, because I know that acknowledging the Lord first will only enhance my walk with the Lord.
So yes just like you I might be tired and don't feel like reading my bible, or maybe I might not feel like praying because like I said things happen but I re-group and I acknowledge Him and I eliminate what is in my way at that moment to make time to read my bible and pray.
Acknowledging the Lord is acknowledging Him in everything in our lives. His word promise that when we do our paths will be straight. I rather walk the straight path then walk a crooked path.


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Dr. A. Rajkamal Goud
MBBS, DNB(Internal Medicine)
Consultant General Physician
About Dr. A. Rajkamal Goud
Dr. A. Rajkamal Goud completed his MBBS and DNB in Internal Medicine.He has 2 years of experience and He worked as a consultant general physician in Bangalore(1 1/2 yr.).
- study of hypokalemia in hospitalized patients (2015).
- Got gold medals in MBBS (Anatomy, Bio-Chem, fusio, SPM, Plasma, Micro,Paluo).


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Tyres in Newbridge
Conveniently located in Broxburn, R&J Strang Tyre Services Ltd is easy to reach from Newbridge. Carrying one of the widest ranges of tyre brands and sizes in Broxburn you can book online (pay in store), call us or simply drive in. For your convenience we've provided driving instructions from Newbridge to our location in Broxburn.


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Automotive Repair in Eagleby, QLD
The most popular Automotive Repair near Eagleby, QLD, 4207
The most popular Automotive Repair near Eagleby, QLD, 4207
Fast, affordable, and reliable Auto Repair in Brisbane. We are Car Mechanic Experts offering All Around and Complete Car Service, Maintenance, and Repairs. Log Book Car Servicing available. Same Day Mechanic Service. Any models are welcome.
Phone: (07) 3392 6972 | Make an Enquiry: #1 Best Car Service in Brisbane | Servicing: Eagleby
75 Sunscape Dr, Eagleby, QLD, 4207
Shed 1, 14 Reservoir St, Beenleigh, QLD, 4207
Beenleigh Batteries is a family owned business that has served Beenleigh, Logan and the surrounding areas since 1988.more
1 Kalimna St, Loganholme, QLD, 4129
Mobile, Mount Warren Park, QLD, 4207
Our technicians repair all types of alloy wheel damage. If you've damaged your wheels and need alloy wheel repair, gutter rash rim repair, curb damage, face alloy wheel and alloy wheel scratch repairmore
24 Dulwich St, Loganholme, QLD, 4129
7/101-107 Logan River Rd, Beenleigh, QLD, 4207
Unit 17 34 Pacific Hwy, Yatala, QLD, 4207
48-50 Cairns St, Loganholme, QLD, 4129
240 Peachey Road, Shed 22, Yatala, QLD, 4207
Dynamic Diesel Services are the go-to Gold Coast and Brisbane heavy vehicle mechanic for all truck repairs, trailers, earthmoving equipment repairs and more!more
41 Quarry Rd, Stapylton, QLD, 4207
41 Quarry Rd, Stapylton, QLD, 4207
41 Quarry Rd, Stapylton, QLD, 4207
Cnr Pacific Hwy & Elliott Drv, Yatala, QLD, 4207
Unit 6, 11 Commerce Circuit, Yatala, QLD, 4207
Currey Customs and Mechanical is an automotive and performance tuning workshop for vehicles, offering the following services: logbook servicing and maintenance for all petrol and diesel vehicles suspension, brakes, engine, gearbox and driveline repairs RWC inspections (roadworthy and safety certificates) electronic fault diagnosis and repairs full customisation, race packages and performance tuning for 4/6/8 cylinder engines turbo installations for GOAT performance products; twin turbo kits for Holden Commodore VT-VF 6 and 8 Cylinder Models dyno tuning classic car restorations and builds Currey Customs and Mechanical has over 15 years of experience in the mechanical industry and was founded by Chris Currey, a highly regarded mechanic and classic car restorer from Brisbane. Chris's passion and expertise is servicing, restoring and customising American muscle cars from the 50s through to the 70s. So whether your vehicle needs a service, a diagnosis and repair, performance tuning or a complete custom build or restore, Chris and the team at Currey Customs and Mechanical can look after all of your needs!more
Cnr Pacific Hwy & Elliot Drv, Yatala, QLD, 4207
Unit 1 14 Glasson Dr, Bethania, QLD, 4205
1462 Ipswich Rd, Rocklea, QLD, 4106
We are one of Queensland's leading car wreckers, located in Brisbane. We recycle all types of scrap metals including unwanted cars, 4×4 Wreckers Brisbane, buses, vans, motorbike and trucks.more
1770 Mt Cotton Rd, Carbrook, QLD, 4130
By Appointment, Mount Cotton, QLD, 4165
We specialise in the removal of hail damage, minor body dents, everyday door dings and those annoying dents caused by shopping trolleys and car park mishaps. Your car will be restored to its pre-dent condition. It will look like the dent never happened! We come to you! We are a mobile service that can repair your dents at a location convenient to you, whether it be at home, work or elsewhere. On most occasions we can offer a same day service, operating from 7am to 7pm, 7 days a week. The cost to remove the dents from the panels of your car using Paintless Dent Removal is much less than a panel shop repair and in most cases, will be less than your insurance excess. Furthermore, your car will be repaired in a fraction of the time. Most dents can be repaired on site, while you wait. We service in all sectors of the automotive industry, including privately owned cars, fleet cars, car yards and dealerships, panel shops, insurance companies and rental car companies. Our comprehensive understanding of all types of vehicles and the latest dent removal techniques ensures you receive the highest quality service every time. We offer a reliable, cost effective, environmentally friendly service and a lifetime warranty on all PDR repairs. Customer satisfaction is our priority. For a free quote, please call or send a photo to us. Alternatively, you can complete the online enquiry form at our website.more
314-316 Loganlea Rd, Meadowbrook, QLD, 4131
Unit 2, 1-15 Beal St, Meadowbrook, QLD, 4131
Junair specialises in commercial and industrial spray booth equipment and maintenance across Australia and New Zealand. Besides selling spray booth equipment, they offer services such as mechanical & electrical equipment, service and maintenance, and more.more
46 Railway Pde, Loganlea, QLD, 4131
46 Railway Pde, Loganlea, QLD, 4131
181 Queens Rd, Kingston, QLD, 4114
181 Queens Rd, Kingston, QLD, 4114
180 Queens Rd, Kingston, QLD, 4114
173-177 Queens Rd, Kingston, QLD, 4114
Unit 1, 17 Moss St, Slacks Creek, QLD, 4127
Trusted Mechanic, Low prices, Family Owned and Excellent Workmanship. We value our Customers and their Vehicles so we make sure that you get back on the road ASAP with comfort and ease of mind.more
Po Box 700, Ormeau, QLD, 4208
Jual Court Unit 4 3489 Pacific Hwy, Slacks Creek, QLD, 4127
Southside Brisbane, Logan Central, QLD, 4114
9 Aranda St, Slacks Creek, QLD, 4127
13 Aranda St, Slacks Creek, QLD, 4127
Horizon Shores Marina Cabbage Tree Point Rd, Woongoolba, QLD, 4207
35 Watland St, Springwood, QLD, 4127
32 Waratah Dr, Crestmead, QLD, 4132
116 Aquarius Dr, Kingston, QLD, 4114
3405 Pacific Hwy, Springwood, QLD, 4127
Carters Car Care is an auto repair and diagnostics shop that is situated in the Springwood area. Carters tells customers that if they are looking for a mechanic to trust, then they can stop looking. Carters has an unrelenting commitment to quality and customer service, evidenced by desirable features like logbook servicing, transparent pricing and affordable prices. Carters also is an advocate for preventative maintenance since they want their customers safely on the road for as long as possible.more
3376 Pacific Hwy, Springwood, QLD, 4127
Wide range of new Toyota cars available.
45 Randall St, Slacks Creek, QLD, 4127
Panel House is an Australian owned and operated crash repair parts company with thousands of items in stock and ready to go.more
45 Randall St, Slacks Creek, QLD, 4127
Unit 1 40 Randall St, Slacks Creek, QLD, 4127
7 Dan St, Slacks Creek, QLD, 4127
Unit 3, 40 Randall St, Slacks Creek, QLD, 4127
No matter what your cars situation is, you have come to the right place for advice, affordable prices (no hidden costs), qualified mechanics (no apprentices) and 100% customer care.more
9 Herbert St, Slacks Creek, QLD, 4127
86 Moss St, Slacks Creek, QLD, 4127
5 Murrajong Rd, Springwood, QLD, 4127
28 Moss St, Slacks Creek, QLD, 4127
3 Monte St, Slacks Creek, QLD, 4127
As your local Mechanic in Slacks Creek, Kiwi French Automotive take great pride in offering our clients the very best in car servicing and automotive repairs.more


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Angels acquire Arizona ace Haren
2nd player hit in head by foul ball
Dodgers pitcher Carlos Monasterios was struck on the right side of the head by a foul ball off the bat of Carlos Beltran while sitting in the Los Angeles dugout in the fourth inning of a game against the Mets on Sunday.
A Dodgers spokesman said that Monasterios never lost consciousness. Monasterios was examined by team physician Neal ElAttrache and appeared to be doing fine, adding that Monasterios does not appear to have any signs of a concussion.
The accident occurred less than 24 hours after Giants outfielder Eugenio Velez was struck on the left side of his head by teammate Pat Burrell's foul ball while he was in the dugout during a game against the Diamondbacks. Velez sustained a concussion and spent the night in the hospital.
Manager Bruce Bochy said Sunday he was doing well and was expected to be released before the team heads back to San Francisco.
The Giants placed Velez on the 15-day disabled list.
Dan Haren was traded to the Los Angeles Angels by the Arizona Diamondbacks on Sunday for left-hander Joe Saunders and three other players.
The last-place Diamondbacks also get right-hander Rafael Rodriguez, minor-league pitcher Patrick Corbin and a player to be named.
A three-time All-Star, Haren was one of the most prized pitchers available as the July 31 non-waiver trade deadline approaches. He joins a Los Angeles rotation that is solid up front but is missing injured left-hander Scott Kazmir.
"A top-of-the-rotation guy that's going to help us not just this year but years down the road," Angels General Manager Tony Reagins said.
Haren has averaged 15 wins over the past five seasons and is 7-8 with a 4.60 ERA in 21 starts this year. He was tied for the NL lead with 141 strikeouts through Saturday's games.
Saunders, an All-Star in 2008, was 6-10 with a 4.62 ERA in 20 starts for the Angels this year.
Short hops
■ ESPN analyst and former Mets and Rangers manager Bobby Valentine said he's interested in taking over for retiring Cubs manager Lou Piniella after this season. Valentine said the Cubs job was "one of the most coveted positions in all of sports." Valentine spoke before Sunday's game between the Cubs and Cardinals.
■ Carlos Zambrano will likely make one more appearance with Triple-A Iowa before rejoining the Cubs next weekend. He will probably return during their series at Colorado, Piniella said.
■ The Tigers placed outfielder Magglio Ordonez and second baseman Carlos Guillen on the 15-day disabled list Sunday. Ordonez fractured his right ankle and Guillen strained his right calf in Saturday night's 3-2 loss to the Blue Jays.
■ Orlando Hudson was placed on the 15-day disabled list by the Twins, who wanted to give their second baseman sufficient time to recover from a strained muscle in his right ribcage.