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The Fear of Fear Itself
My therapist has a gray suit and gray ponytail — no tie. "How are you feeling today?" he asks. Gray, I want to say, like your hair. Or maybe blue, like the faded 1970's wallpaper crackling off the walls. Instead, I just say, "Better this week!" I hope my smile was convincing enough. At that point I wonder why I'm even here if I'm not going to tell him the whole truth. I think about how pathetic I am that I need to embellish my life for this old man who probably sees people regularly who are way "worse" than me. The anxiety, always present these days, bubbles up from my stomach and takes its place solemnly in my throat.
I never thought I'd be a person who has to go to a weekly therapy session. I grew up comfortably middle class. My parents were together. I had healthy friendships. I did well in school. I graduated Cum Laude from college while juggling internships and a job toasting bagels and calming down outraged customers at Panera Bread. I was "stable." I was a calming presence most of the time. A friend even once told me, "Laura, you're like the chillest person I've ever met." I considered myself someone with my life put together. As I glanced over at the hideous wood paneling on one of the other walls in the room, I thought about how I earned As in grad school, had a great full-time job, was married to a wonderful man, and also struggled with debilitating anxiety and depression.
A dog barks outside. Incessant and unrelenting, over and over. It just won't shut up. I'm painfully reminded of my mental illness as the therapist asks if I was able to make time this past weekend for anything fun and stress relieving. I talk about visiting an old friend, spending time with my horse, and finally getting my house cleaned up. I purposely neglect to mention the fact that I retreated to my bedroom and closed the door at 7:30 p.m. on Saturday night without saying a word to my husband. "I've just been feeling a little tired," I offer guiltily.
"I'm sure it's hard," my therapist responds. He closes his eyes and smiles. He is always closing his eyes when he is speaking to me. It's terribly distracting. The thought flickers through my head that maybe he just can't stand to look at me, but it fizzles out quickly. I know it's silly, but anxiety does things like that to you. "You're almost finished school though, right? Just a few more months to go!" I wait for him to open his eyes and look at me before telling him I'm worried my anxiety will get even worse after my graduation. The nagging feeling that I have something I'm forgetting to do will probably worsen when I have more free time. I don't know how to cope when I don't have a million things on my mind, either. "I know that feeling," my therapist says gloomily.
A clock tick-tocks above my head. I hear it in between the shrill barks of the dog outside. I desperately look around the small room for an escape. Therapy sessions aren't like what you see portrayed on TV or in the movies. You don't lie down on a couch in a stark white room. For someone with anxiety, it's not even particularly relaxing. I've been seeing this man for weeks now and still have to fight panic when I enter that building. I wonder why they don't try to make the environment more comfortable for their patients. I wonder what I'd even consider to be a comfortable environment to undergo counseling for my anxiety disorder and decide to give them a break. There is no place I could imagine that would make the experience more pleasant.
Anxiety feels like that moment when your chair is about to tip over backwards and you don't know if you'll be able to catch yourself or not. It feels like when you check your pockets — phone, keys, wallet? — and they're not there. Anxiety lights your mind ablaze, making you wonder if you're adequate enough, if you can make it through another day. It forces you to relive that one embarrassing thing you did three months ago on a nightly basis. The worst part is that you know the entire time that you're overreacting — like when I had to pull my car over to calm down when I thought about how I really needed to buy a new toaster oven and the thought of going to the store and picking one out sent me over the edge. Franklin Delano Roosevelt said it best: "The only thing we have to fear is…fear itself — nameless, unreasoning, unjustified terror which paralyzes needed efforts to convert retreat into advance." Anxiety is the fear of fear itself.
I told my therapist about my struggle to buy a toaster oven last week, knowing it was his job not to judge me for it, but still internally cringing at myself for bringing it up. I never actually did go buy one. I thought about my therapist going home after our session and laughing with his wife about the patient he saw today who was anxious about driving to Walmart and picking out a new toaster oven. "I can't believe I get paid to coach this poor mentally ill girl on how to handle her life. She can't even walk into a Walmart without feeling anxious. What a joke!" he would say to her. Of course, I knew that was absurd of me to think, but the thoughts rose up and exploded in my head like dynamite all the same.
My therapist was closing his eyes again. I looked down at the floor. "Sometimes," he told me, "we often push our stress aside, to the back of our minds. When we do that, we are able to cope with our day-to-day activities. However, the stress doesn't go away. It just piles up higher and higher back there until suddenly it spills out at inopportune or inappropriate moments. That's why you feel anxious about these tiny, everyday events." It was a sensical thing to say. I weighed his words in my mind and felt a little part of the anxiety nestled in my throat make its way back to the pile of stress sitting in the back of my head. Maybe it wasn't a quick fix, but it felt good to have my anxiety validated out loud. So often I punished myself for being unable to do simple tasks. My therapist was telling me it was OK — he understood me.
The dog was still barking and the clock was still ticking and the wallpaper was still peeling but my mind had stopped racing. At least for now. When you're living with anxiety, it can be difficult to explain yourself to people, both figuratively and literally. Sometimes, getting up the strength to make a simple phone call can take all day. It took me months before I was able to pick up the phone and schedule myself an appointment with a therapist. And because there is such a stigma still surrounding mental illness, it can be embarrassing and scary to explain to people why you canceled plans or why you're crying hysterically for no apparent reason. So to just have simple validation and a valid explanation offered for why I simply could not handle my life felt — dare I say it? — therapeutic. I had been craving it all this time without really knowing.
"Thank you for not saying, 'It's going to be OK,' or 'Calm down,' or 'It's not that serious,'" I told my therapist. I hear all of those phrases a lot. Mostly, it's me speaking to myself. But occasionally, it's a friend or family member. All those cliche phrases — Think positive! Live each day like it's your last! Don't worry, be happy! — are the absolute worst. Anxiety manifests without warning and sometimes without cause, and once it takes its hold on me, no amount of positive thinking can pry me from its grasp.
"Well, it looks like our time is up today," my therapist said, rising from his chair. I followed him obediently to the door, still eager to get the hell out of that room. As I stepped out into the hallway, fresh air hit me and filled my body. I'm not generally a claustrophobic person, but that room brought out the worst in me. I tell the receptionist outside, "Same time next week!" and thank my therapist before quickly exiting the building. While walking to my car, I thought about the session. Was being stuffed into that terrible blue room with that gloomy gray man really helping me? It was nice to have a stranger validate my feelings, but what I really needed was self-validation. I thought about dropping out of school. I thought about adopting another cat. I wondered if my husband ever thought about divorcing me. I thought about how much food I was going to stress-eat when I got home. I sat down in my car and practiced my deep breaths that my therapist had taught me before turning the key in the ignition and driving away.
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I'm sure there is something better on it… Damn, what's that banger ?!
Pete Rock feat. Roc Marciano and Trife : Give it to y'all », at the
time I get crazy on that track in the record shop, with the huge
headphones on my head. Aight, I'm buying it just because of this track, «
The beat generation » by Pete Rock (even tho the rest of the CD is well
good too)…
Roc Marciano, this guy kills mics, after some solo tracks, he gets involved in the Flipmode Squad… Roc Marciano will quit the crew a bit later on, but had the time to drop some bangers such as :
I let you looking for more information yourself, so you can (as I did) find some dope shit involuntary thanks to youtube and the other websties!
Peace !
PS : As well, he released « The pimpire strikes back » mixtape this year…(PS : As well, he released « The pimpire strikes back » mixtape this year)
To follow Roc Marciano : @RocMarci

subset: commoncrawl


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Rams vs Bengals Odds - Rams to squeak past hobbled Bengals
Rams vs Bengals odds: Bengals -2.5
- WHAT: NFL betting
- WHEN: Thursday, August 27th – 7:30 PM ET
- WHERE: Paul Brown Stadium – Cincinnati, OH
- PICK: Rams +2.5
Rams vs Bengals odds maker breakdown: Offense Last season, the Rams had all the talent parts of the offense but were lacking a good offensive line. After only one offseason, the Rams are now at the other end of the spectrum. The Rams offensive line has looked very strong this offseason, but the quarterbacking and receiving have been suspect. They'll have an even matchup against an improving Bengals defense on Thursday. The Bengals will likely be without Carson Palmer for a second consecutive week as he deals with his high ankle sprain. The Bengals offense has moved the ball up and down the field well this preseason, but they haven't been able to convert those long drives into points. That likely won't improve without Palmer. Betting Edge: Even
Rams vs Bengals odds maker breakdown: Defense The Rams have a very underrated defense, especially now that defensive guru Steve Spagnuolo is running the team. There's plenty of talent with former first-round picks like Adam Carriker and Chris Long, and newcomers like James Laurinitis and James Butler should also bolster the roster. The Bengals are very similar in the sense that they have a lot of talent that just hasn't jelled quite yet. They showed some signs, though, when they managed to hold the New England Patriots to just six points on the road. At this point, they get the edge. Betting Edge: Bengals
Rams vs Bengals odds maker breakdown: Special Teams NFL betting fans that watched Chad Ochocinco kick an extra point and a kickoff last week may have been impressed, but the reality is that the Bengals are without starting kicker Shayne Graham and are shorthanded at the position. Graham is questionable. Given that injury, the Rams have the edge on special teams. Betting Edge: Rams
Rams vs Bengals odds maker breakdown: Notable Injuries As mentioned, the Bengals will likely be without Carson Palmer and Shayne Graham, which is not good news for a team that's only scored 14 points in the preseason so far. Both of those players directly impact the teams point totals and missing them will be a problem. Betting Edge: Rams
Rams vs Bengals betting breakdown: Outlook and Pick The Bengals are just under a field goal favorite in the sportsbook but the Rams look like the better play as an underdog. The Bengals are without their starting quarterback and placekicker, and they've already had trouble scoring in the preseason. Look for the Rams to capitalize as they get by with a win. STAN'S PICK: Rams +2.5
For more fantasy news and analysis on sports betting, check out the betonline.com Betting Edge daily. Stan Simmons first tasted football through the college game, but his football handicapping has strictly focused on the professional sport. Not many people devote as much time to the NFL as Simmons. For six days a week he works, labors and handicaps the sport, and on the seventh he rests as the games play out. Thoughts or questions?


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This page is to provide information about what you will have to do in attempting to raise a caterpillar to its final adult stage of butterfly or moth. Since different species require different conditions, I will change this page from time to time to provide information gained from my own experience with various Lepidoptera (butterflies and moths).
Check out my new post on spicebush swallowtails. These are interesting to raise because of color changing as they mature. Note the fake eyes and the threatening posture. The combination would scare off many would be predators ! Then, for further protection, they hide in "tents" they make from the spicebush leaves they feed on.


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With one exception — a big one — we favour expanding Ontario ombudsman André Marin's oversight to include municipalities, school boards and universities.
Politicians and bureaucrats working in these sectors have long operated free of independent scrutiny. Inexcusable excesses have resulted, usually at taxpayers' expense, especially at the civic level.
Scandals abound. The Toronto District School Board, alone, offers a wealth of examples, such as spending $2,900 for an electrical outlet on a library wall. Sudbury generously provides each of its city councillors a $50,000-a-year slush fund to be distributed as they wish. And, of course, police are investigating the widespread flouting of spending rules in Brampton.
In that city, formal approval of expense accounts was rolled back "in favour of the honour system," Marin told the Star's editorial board this past week. "That worked out well," he added, with a sarcastic tone.
Even more worrisome than the abuses we know about now is what else may lie below the surface. Simply put, in the absence of permanent, credible oversight the public has no reliable way of gauging the extent of possible rot at the civic level.
To close this accountability gap, the province is expanding the jurisdiction of Marin's office to cover all 444 of Ontario's municipalities. It's part of legislation re-introduced by Premier Kathleen Wynne's government in July.
Once Bill 8 is passed, people in towns from Kenora to Kingston will be free to file a formal complaint to the Ontario ombudsman if they see wrongdoing or feel mistreated by their municipality. On the whole, that's a good thing.
When the legislation was first announced, Marin noted it would correct a situation that has left "millions of citizens with nowhere to complain about the public bodies that touched their lives most closely."
But there's a problem: Bill 8 goes too far.
It needlessly covers millions of citizens, specifically in Toronto, who already have an effective ombudsman fielding complaints.
Under the new legislation Marin's jurisdiction would extend to encompass Toronto ombudsman Fiona Crean — so he'd be an ombudsman overseeing the work of another ombudsman.
Marin argues that he'll leave Crean free to pursue picayune local complaints while he handles big investigations into "systemic" civic problems. But Crean has done an exemplary job on both fronts, recently exposing systemic failures at the Toronto Community Housing Corp. that resulted in the well-deserved firing of CEO Gene Jones.
Marin's intrusion sets the scene for turf wars, jurisdictional confusion and bureaucratic delay. Jones may have kept his job for months longer had Mayor Rob Ford, seeking a fresh investigation, complained to Marin's office about Crean's in-depth report. Indeed, Ford almost certainly would have done that.
Marin expressed confidence that he would have no problem effectively overseeing this city's ombudsman's office, just as he handles various provincial bodies. "Toronto's not that special," he concluded,
But he's wrong. This city, alone in Ontario, has extraordinary autonomy through its own legislation: the City of Toronto Act. The act requires Toronto to have an ombudsman — that's why only this municipality employs one. And this office should not be superseded.
While Bill 8 brings welcome and necessary accountability to the rest of the province, it risks clouding and even undermining this worthwhile objective in Toronto. Queen's Park has been reluctant to exempt this city because smaller communities may seek to escape supervision simply by hiring their own ombudsman. But that concern is readily answered.
The correct next step is to amend Bill 8 so that it exempts, from Marin's purview, any municipality that has an ombudsman as well as special rights and powers enshrined in its own, unique legislation. As Canada's biggest city and sixth largest government, Toronto is not like any other place in Ontario. It is special. And it would be a capital mistake to ignore that.


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It's the eyes that first give them away.
My name is Minowski, and thanks to my inability to keep my mouth shut when it's good for me, I'm sheriff of Io-31 Spaceport. They founded Io-31 when mercury mining was still going strong. It didn't last, and within a year the place had become the last outpost of law and order in the solar system, with every reject from the Jupiter colonies winding their way there to try and get a way back to earth. Some made it, some didn't, but every last one of them would kill you as soon as look at you.
So one night I'm having a quiet drink in Sizlack's bar. I've spent the day listening to people telling me they're seeing ghosts near one of the abandoned mines. Turns out there's some sort of gas being produced by chemicals in the rotting machinery that's making people see things. All I want to do right now is get half a bottle of sythecol inside me and hit the sack.
But then I spot them – four kids, two guys and two girls, sitting in a booth in the corner. Now kids, we don't see many kids at Io-31. So I figure, these are either runaways, or they're on the game, or both. They don't look like sex workers, though; they're dressed just like ordinary kids. So they're runaways, or they're grifters, or…
Then I see the eyes. A little too settled, a little too knowing. Too old. These aren't kids at all. And that doesn't leave many possibilities, and I don't like any of them.
The pretty girl stands up and walks to the bar. She's well groomed, slender, and poised, just the opposite of her frumpy friend. She touches the arm of a man standing there drinking, and whispers something in his ear. He can't believe his luck and motions to Sizlack to bring her a drink. She opens her purse as if to pay but he stops her; it's a nice touch on her part, I think, whatever the grift is. Because this has to be a grift.
The three at the table are watching her as we drink. They don't say anything; they're just biding their time. Then the bigger guy gets up. He's got a footballer's build, looks like he can bench 220. Suddenly, his face is all thunder, and he stalks up to the couple at the bar, who by now are all over each other, and taps the guy on the shoulder. The guy turns around, first curious, then concerned, then defensive as the blond kid starts to get wound up.
So this is how it's going to go. The blond kid is going to take a swing at the guy. The guy is going to go down. The girl is going to get his wallet. Sizlack is going to throw the kids out so by the time the guy comes to, they're miles away.
I walk across the bar to where the action is about to go down. Blondy is just pulling back his arm to throw a punch. I step between him and the mark and grab his fist.
He's ice cold, like there isn't a drop of blood in his body.
I've found it's best to stay calm and friendly in the face of trouble. "My name is Minowski, and I'm the sheriff here. I just thought I'd say hello and – "
I already know what I'm dealing with. I don't need to be proven right by him throwing me across the room like I was a rag doll. Still, it's always nice to be proven right.
I land on a chair near the booth where the frumpy girl sits waiting with the lanky boy who looks like he's going to need to start shaving real soon, but not yet. It crosses my mind, as the chair shatters beneath me, that it must really piss him off to be caught in the middle of puberty like that.
Across the bar, Blondy weighs up his options. "C'mon, gang," he shouts, heading towards the door and pulling the pretty girl with him. The two in the booth stand up and the girl follows quickly. The lanky guy hesitates to let her pass. I grab a broken chair leg and jump at him, pinning him back against the wall of the booth, the sharp end of the wooden leg poised over his unbeating heart. He looks down, terrified.
"Like I was telling your buddy," I say, "I'm the sheriff here, and I think you and I should have a little talk."
He thinks about it. I think the truth is, he really wants someone to talk to. People don't know how lonely it is living on the wrong side of the law. And my guess is that this guy is pretty much the runt of the litter where his gang are concerned. He relaxes and nods. I motion to Sizlack and a moment later there are drinks on our table.
"You got some ID, kid?" I ask. He fumbles in his pockets. "Forget it," I say. "It's gonna be fake anyway, isn't it?"
He says nothing for a while. "How did you know?" He casts his eyes to the chair leg then back to me.
"That you and your friends are vampires? Partly because your friend threw me across the room with one hand. Partly because he don't have a blood supply. And partly because you might look like you're sixteen but your eyes… your eyes have seen way, way too much."
He gives a hollow laugh.
"Most people don't believe in vampires."
"I know different. I know you don't see them in the bright lights of the big cities. But my family had – dealings with them back in the old country. And in the shadows of a place like this, it's no surprise to find – "
"We're not killers, right? Like, I can hardly even stomach blood."
"I believe you. So humour me. How old are you?"
"Next month I'll be exactly one hundred."
"So, born 1953. A good year." I raise my glass in a toast and he laughs again. "What's your name?"
"Norville. Norville Rogers."
"Well, Mr Rogers, what are you doing in my spaceport?"
My mark of respect wrong-foots him, and he drops his guard a little.
"We're looking for something. Someone." He takes down most of his drink in a single gulp.
"Tell me about it. Maybe I can help you find them."
"It's a long story," he says, and drains his glass.
I motion to Sizlack again. "I'm in no hurry. And if it gets you troublemakers out of my sight quickly, I'm willing to help."
"Okay, like, it's 1969. My pal Freddy Jones gets given a big old van by his dad when he gets through drivers' ed. We think, hey, let's drive up to coast to Frisco and see the sights. So, like, Freddy's got a thing for this chick Daphne Blake, and she's got this friend Velma Dinkley who we're sure is a dyke but is kind of funny, you know? And my best pal, Robbie Scoble, he's been to Frisco before and knows some people there. So we all get in the van and head north.
"And it's great. We have like the best time in the world. And then we meet these guys in a bar who invite us to a frat party in Sunnyvale. They give me some weed and it's primo stuff, man. So I really want to go to this party. Robert, he's got a bad feeling about it, he's heard that Sunnyvale is a pretty nasty place. And he's my best pal, so I really want to listen to him, you know? But I really want some more of that weed. In the end, he gives in, and we go.
"And the place is like, full of freaking vampires. We didn't stand a chance, man. They turned us all. Freddy. Velma. Daphne. Even my best pal, Robbie. Man, I cried. I'd known that dude since kindergarten and I had to watch while they drank every last drop of his blood.
"It takes us a couple of days to get our act together. We knew it would be tough to go home. Hell, we knew it would be tough to do anything. We couldn't even, like, go out in the daytime. Then one morning, Velma comes back to the van. She's been gone all night and she's, like, really excited. She's met a witch who can help us. And I'm like, Velma, you don't believe in witches and she's like, I don't believe in vampires either so fuck you.
"So we go see this witch, right? It turns out she's a priestess of some trickster god, and this trickster god has an enemy he wants killed. And if we find this enemy, and kill him, this god will make us human again. So we're like, how do we find him? And the witch tells us that this guy is into the whole frighteners scene and we have to look around for people being scared, spooked out, and generally terrified by things that go bump in the night. So we're like, how do we do that when we can't even go out in the day?
"So she casts this spell, which like – like, she fixes it so we can go out in the daylight and we don't need to drink so much blood and we can get by on ordinary food, so long as we eat a lot of it. Like, then we start on the world's longest road trip, looking for this trickster god's enemy. We chase down every spooky mystery we can find, and almost every time it's some nut job in a mask hiding a pile of loot.
"We built up quite a following, you know? After a while we could tell the cops anything and they'd believe us. No-one seemed to worry that we never got any older or we had a dog who could freaking read. This one time, we brought down the mayor of Winterhaven. He was running a crazy property scam with one of his buddies dressing up in a wetsuit and sabotaging the local power plant. So, like, we figure out what's going on and we're thinking no-one is ever going to buy this. But we go to the police with this crazy plot, right, and they're like, no problem, it's you guys, and they just, like, arrest the mayor on the spot. The mayor, dude.
"That's when Velma realised we could get away with anything. She really is the brains of the gang. I mean, Freddy has his plans, and people, well, people think I'm funny, but it's Velma who makes things happen. Anyway, Velma goes quiet for a few days, and then says we're going to Sleepy Hollow. Next thing we know, we're chasing after a headless horseman who turns out to be a businessman stealing a diamond necklace because he's going bankrupt. We catch him, and he starts saying we've double-crossed him.
"Double crossed? Well, yeah, because then Velma tells us that she planned the whole thing and put him up to it, told him we'd lend some cred to the whole headless horseman thing. In the meantime, she's made a stack doing some kind of crazy deal with his company's stock. So now he's totally ruined and Velma sells the company's assets. And we're like, rich, man. Which is good, because hamburgers were getting real expensive back then.
"Velma looks after us pretty good, but sometimes money gets tight and we have to hustle a little – like you saw."
"So you're here because of what's going on in the abandoned mine?"
He nods.
"I can save you some time there. There's no nut-job but there is a lot of dangerous gas which is making people see things. I don't think your god's enemy is here."
I see the look of dejection on his face. They've been searching for almost a century and I've just handed them another dead end. Then I see a flash of blond at the door to the bar. "Still," I say, "your friends are."
He waves and motions them to join us. As the three kids walk over, I see they have a dog with them, a Great Dane, who bounds forward joyfully towards us, running to Norville's seat and putting his front paws up on Norville's legs, nuzzling into his neck before settling back down.
"You never told me," I say, "what happened to Robert."
He pauses. "The spell – the witch said that if one of us would give up the last of their humanity, it could be shared with the others to make them almost human again." He takes hold of the dogs head and looks affectionately into its eyes. "Only Robert Scoble was brave enough to make that sacrifice." He turns to face me. "We call him Scooby, now. And, like, he's my best pal."


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Strand-specific libraries for high throughput RNA sequencing RNA-Seq prepared without polyA selection
Strand-specific libraries for high throughput RNA sequencing RNA-Seq prepared without polyA selection - Download this document for free, or read online. Document in PDF available to download.
, 3:9
Non-coding RNARNA: Methods and Protocols
BackgroundHigh throughput DNA sequencing technology has enabled quantification of all the RNAs in a cell or tissue, a method widely known as RNA sequencing RNA-Seq. However, non-coding RNAs such as rRNA are highly abundant and can consume >70% of sequencing reads. A common approach is to extract only polyadenylated mRNA; however, such approaches are blind to RNAs with short or no polyA tails, leading to an incomplete view of the transcriptome. Another challenge of preparing RNA-Seq libraries is to preserve the strand information of the RNAs.
DesignHere, we describe a procedure for preparing RNA-Seq libraries from 1 to 4 μg total RNA without polyA selection. Our method combines the deoxyuridine triphosphate dUTP-uracil-DNA glycosylase UDG strategy to achieve strand specificity with AMPure XP magnetic beads to perform size selection. Together, these steps eliminate gel purification, allowing a library to be made in less than two days. We barcode each library during the final PCR amplification step, allowing several samples to be sequenced in a single lane without sacrificing read length. Libraries prepared using this protocol are compatible with Illumina GAII, GAIIx and HiSeq 2000 platforms.
DiscussionThe RNA-Seq protocol described here yields strand-specific transcriptome libraries without polyA selection, which provide approximately 90% mappable sequences. Typically, more than 85% of mapped reads correspond to protein-coding genes and only 6% derive from non-coding RNAs. The protocol has been used to measure RNA transcript identity and abundance in tissues from flies, mice, rats, chickens, and frogs, demonstrating its general applicability.
KeywordsRNA-Seq Transcriptome High throughput sequencing AbbreviationsdATPDeoxyadenosine triphosphate
dCTPDeoxycytidine triphosphate
dGTPDeoxyguanosine triphosphate
dTTPDeoxythymidine triphosphate
dUTPDeoxyuridine triphosphate
NEBNew England Biolabs
PCRPolymerase chain reaction
PEGPolyethylene glycol
piRNAPiwi-interacting RNA
RNA-SeqRNA sequencing
RNase HRibonuclease H
rRNARibosomal RNA
snoRNASmall nucleolar RNA
snRNASmall nuclear RNA
SOCSuper optimal broth with catabolite repression
T4 PNKT4 Polynucleotide Kinase
UDGUracil-DNA glycosylase.
Electronic supplementary materialThe online version of this article doi:10.1186-1758-907X-3-9 contains supplementary material, which is available to authorized users.
Download fulltext PDF
Author: Zhao Zhang - William E Theurkauf - Zhiping Weng - Phillip D Zamore
Source: https://link.springer.com/article/10.1186/1758-907X-3-9


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Welcome to the new ASER Website!
Your Board has been hard at work on a new website during the past six months.
Enhanced Recovery
Surgical recovery can be optimized with evidence-based pre-operative, intra-operative, and post-operative interventions. Patient education, nutrition, and preconditioning combined with intraoperative and postoperative standardization can improve patient safety, enhance quality of care, advance outcomes, and speed recovery, all while optimizing resource utilization and satisfaction. "Healing is a matter of time, but it is sometimes also a matter of opportunity" – Hippocrates
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FINALLY!! The weekend we have all been waiting for…tomorrow is the BIG GAME (due to copyright laws I think we can't call it what it is really called, right?)! The Packers and the Steelers apparently brought the cold weather, snow and ice to the South when they descended on the city of Dallas en masse earlier this week. Parties galore, big events and falling ice? Someone must really be unhappy with the Dallas Cowboys season to bring all of this wrath on Dallas.
I realized a few days ago that on Sunday it is possible that the Pittsburgh Steelers will win their seventh Super Bowl ring…in Cowboys Stadium. Something just seems so wrong about that idea. I have heard more than one Cowboys fan this week ask, "Can both teams lose?" Big props to Troy Polamalu though for being named the MVP of the season earlier this week. Funny jokes all around about the battle of the hair, as Clay Matthews was the runner-up for MVP honors. Looks like Head & Shoulders won this round, but will Suave win out on Sunday?
In case you didn't catch it earlier this week, one of our owners, Michael Tashnik did a fantastic guest spot on KEYE's "We Are Austin" showcasing how to make some of our favorite appetizers at Third Base. However, we all know you do not really want to cook on Sunday, so why not get your party catered? Or better yet, join us at your favorite Third Base location so that we can clean up after the party!
This year also marks the first year since "Wardrobe Malfunction" that the halftime show will feature a current performer. I am definitely looking forward to see what Fergie and the Peas will do to entertain us. What about you? Will you be watching halftime? The commercials? Or are you a football purist and only concerned about the game?


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The Black Watch Castle and Museum have been awarded a special commendation at VisitScotland's esteemed Thistle Awards.
For the past 25 years the Scottish Thistle Awards have honoured businesses who provide an exceptional experience to their customers and lead the way in Scotland's tourism and hospitality industry.
Jim Clarkson, Regional Director at VisitScotland, said: "I'm delighted that The Black Watch Castle and Museum has received a special commendation in the Historic Environment Scotland - Best Heritage Tourism Experience category."
"The team at the Castle and Museum should be proud of their achievements in representing the region at the prestigious Scottish Thistles Awards National Final."
"The Black Watch Castle and Museum offers a unique portrayal of The Black Watch Regiment's history through their excellent displays and collection, and it is a testament to the hard work and dedication by the team in delivering a top experience for visitors that it has been acknowledged in this way."
Anne Kinnes, CEO at The Black Watch Castle and Museum, explains, "We are thrilled to have been recognised in these national industry awards, one of only two special commendations awarded on the night. The team here at the Castle and Museum are committed to providing a world class experience to all our visitors. This special commendation is a tribute to their drive and enthusiasm to share the rich history of The Black Watch with current and future generations."


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Microneedling is a procedure that's growing in popularity among men and women across the globe. It involves the use of very tiny needles to create hundreds of invisible puncture wounds on the top layer of your skin. While it might not sound very comfortable or appealing, it's less uncomfortable than you might expect, and the benefits certainly speak for themselves.
Microneedling is a simple process, but it's one of the most effective non-surgical procedures available. Benefits include all of the following:
• Reduction of fine lines and wrinkles. Wrinkles form when your skin no longer has a sufficient amount of collagen to keep it plump. Microneedling triggers the production of new collagen, which means the appearance of fine lines and wrinkles will improve over time – especially if you have microneedling done regularly.
• Repairing visible scars. Whether you have a scar from an injury, surgery, or even acne, microneedling can help substantially reduce its appearance.
• Reversal of sun damage and hyperpigmentation. Stimulating the growth of new collagen can even help reverse the effects of UVA/UVB damage and the hyperpigmentation that can occur as a result. Women who have pregnancy or hormone-related melasma can also benefit from the reversing effects of microneedling.
• Improving the signs of aging. The number one reason why people across the globe undergo microneedling is to reverse the signs of aging and stop them in their tracks. Loss of collagen is responsible for the appearance of aging skin, so being able to stimulate its production will leave you looking younger and more vibrant.
• Shrinking pores. Last, but most certainly not least, microneedling can even shrink pores, which can improve the overall look and texture of your skin. As collagen production increases, the skin around your pores becomes plumper, thus reducing the size of your pores and improving your appearance.
Microneedling is a wonderful way to enjoy more radiant and youthful skin, and while it's most commonly done on the face, the treatment has been approved for use on the entire body. In fact, preliminary findings from ongoing studies have shown that regular microneedling can even reduce the look of cellulite and stretch marks just as it reduces the appearance of scars on the face.
Nina Gaetano, BS, PA-C, is the Medical Director of Bella Sol Med Spa


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Tweedsmuir Camp as Accommodation for Polish Families and Dependents (1948 to early 1960)Adapted from Chapter 5 of a book called The Polish Community in Tweedsmuir Camp, Surrey after WWII, Zen and Wies Rogalski, published by The Old Kiln Museum Trust. The book is part of The Tweedsmuir Camp Exhibition at The Rural Life Centre, Tilford, Surrey, England. It is available from The Rural Life Centre, Amazon, Waterstones-on-line and other reputable book sellers.
Families and Dependents
Six days before Churchill made his pledge to the House of Commons on 27 February 1945, he had had an "emotionally charged meeting with General Anders," wrote Keith Sword in 1989. "Churchill was clearly influenced by his encounter with Anders," continued Sword, when he made, what Sword considered to be the sudden pronouncement. Churchill spoke eloquently of "the Polish troops … who had fought under British command." He had hoped "that it may be possible to offer (them) the citizenship and freedom of the British Empire …" His emotions were fully charged when he spoke the last few words on the matter, saying that " … we should think it an honour to have such faithful and valiant warriors dwelling among us as if they were our own flesh and blood." It was a statement that had barely been discussed by the War Cabinet, and certainly not with leaders of the Dominion countries. Yet Australia and Canada, for example, were urged to accept many thousands of men from the Polish Armed Force after the war.
The pledge made no mention of whether the soldiers' families and relatives should be included. Leaving out those who were in Russian and German work camps, there were 396,147 Polish refugees accommodated in camps found in 30 other countries that stretched from Sweden to Australia and Mexico to India. The problem facing the new Labour administration between 1946 and 1948 was which of the civilians should form part of the PRC process and on what grounds were they to be included. To begin with, the title "refugee", and subsequently "displaced person" continued to be used in correspondence and memoranda between British government departments. These terms referred to specific groups of civilians as defined by the military administrators in Germany after WWII. The Polish families who settled in Britain after the war were not displaced people as is often thought, but refugees. Churchill's pledge had to be clarified for government departments. Therefore a more precise definition was required to identify just who exactly Whitehall officials were dealing with. Hence a distinction was made between persons who could be regarded as PRC "family" members, and PRC "dependants." Of course Polish soldiers could not be classified as 'refugees' until they were demobilised.
A family member was the wife, child or children of a PRC enlistee. Other relatives such as uncles, aunts, cousins, mothers and fathers were described as dependants. Therefore, while men in the PRC lived in "Families Camps" with their wives and children, other relatives lived in "Dependants Camps."
There is no evidence to suggest that the ATS/PRS (see Background to the Process of Civilianisation) included married women with children. Had there been then by definition they, their husbands and their children, would have been accommodated in Dependants Camps and not Families Camps.
It may be of interest to know that as well as "families" and "dependants", the Home Office also differentiated between other groups of Polish individuals for whom camps were prepared. For instance, Service Camps/PRC were prepared for single men; Service Camps/ATS(PRS) for single women; Recalcitrant Camps for Polish Airforce, Army and Navy personnel (men) who persistently refused to enlist into the PRC, and Disabled Camps for personnel (men) who had been wounded during the war and whose welfare depended on others.
There is no evidence of Polish orphans at Tweedsmuir even though the war had left many in the care of the Red Cross in countries often referred to as "safe havens" such as Uganda, India, Denmark and Sweden. In the main, the orphans were evacuated to Canada and New Zealand from the country that hosted their stay during the war. Some of them, however, remained in the care of their host country after the war.
During the immediate post war period, Tweedsmuir Camp became a Service Camp for PRC personnel, but was later reclassified as a Families Camp. As such only men (PRC or those demobilised from the PRC) with wives and children were to be accommodated there. However, owing to an administrative oversight, a few grandparents, and in some cases more distant relatives also settled in Tweedsmuir. It is therefore impossible to describe a typical family at Tweedsmuir Camp.
A number of Polish servicemen met Italian women when stationed in Italy after the war. Although these Italian/Polish families arrived in the United Kingdom there is no evidence that any of them settled in Tweedsmuir Camp.


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Olivier El Mekki@oelmekki · maker
Grungiest startup video ever :) I wonder about the main argument for bars : "Gain clientele, Nobody goes to a bar alone for just one beer. Its a simple fact.". Is there anything preventing that potential customer's friends to use the app as well, causing the bartender to have to offer like 10 beers in hope they will take a second one ?
@oelmekki beers can only be redeemed once every 24 hours so that prevents multiple redemptions at once. Bars also have the option of buy 1 get 1 free or buy 2 get 1 free and can put a cap on the number of total redeemable beers in a day.
http://www.fafmag.com/life/food-... Alan from the Hangover is pushing for beer!
Armaan Gupta@katoree · Product at Mapfit
Awesome job with the Zach Galafinakis endorsement! The video reminds me of the Push For Pizza video done by Sandwich Video, but nonetheless good luck fellas!


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Please enter your email address below to get started.
By Lucia Gómez, Ronald Wall and Päivi Oinas
Appendix 2
Worldwide flows of foreign direct investment (FDI) are increasingly targeting countries in the Global South. Understanding what attracts foreign investors to the South helps shed light on how policy can support the countries in making the most of those investments in terms of their developmental impact.
Analyses of global FDI targeting the South between 2003 and 2014 (fDi Markets database) shows that the rising importance of the South in global investment networks is mainly caused by the faster growth of investments either targeting or originating in the periphery (see Figure 1 for the definition of peripheral countries). The analyses further show that the peripheries also attract investments from countries at different levels of development. They are able to satisfy the requirements of investors involved in a range of value-added activities and thereby become entangled in complex global investment networks.


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West Michigan: Pop Or Soda? [POLL]
So here's a question...is it pop or soda? As a lifelong Michigander, I was brought up calling it pop. We had pop in glass bottles. We drank pop at McDonalds. The place was called "Towne Club Pop Centers". Carbonated beverages are just called pop. Fast forward 30 plus years, when I moved to Kentucky. It's not really pop down there. It's coke...little "c". You get a coke, and then they ask what kind do you want. Pop is coke.
A website called "The Atlantic Cities" have a cool map, put together by a scientist at MIT. It shows where the terms "pop", "soda" and "coke" are used across the country. Here it is.
The whole story is here: "Map of the Day: Soda vs. Pop vs. Coke" It's pretty interesting how a simple term like pop or soda can be so defined by a region of the country.


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Hello. I have been slaving over a hot keyboard to bring they guys in our band, and YOU, a brand new and improved web site with better features, easier to use navigation and more streamlined layout. I hope you like it and I hope you will register and leave comments wherever possible!
On behalf of all of US: Thank you!


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The Hail Marys are music veterans of the SF Bay Area music scene and were founded in 1993 by lead singer Jackie Strano. The band has played and toured coast to coast from The Fillmore and Slim's in SF to CBGB's in NYC. Regular fixtures at The Paradise Lounge, DNA, and Pound SF as well as legendary venues like Satyricon in Portland, The Off Ramp in Seattle and Al's Bar in LA, The Hail Marys' songs are featured in soundtracks as well. After the song Hopeless Faith (co-written with Strano by original line up members Peter Hudson and Chris Dean) was featured on the soundtrack of BOUND (The Wachowskis of Matrix and Cloud Atlas fame), the popularity of the band increased. During the quintessential queer years The Hail Marys featured the most well-known line up of the band: Veronica Savage on bass, Debbie Torrey on lead guitar and Pyke Barber on drums. Their indie-produced albums are centered around Jackie Strano and Debbie Torrey's songwriting. When playing live, the 4 of them together produce a sonorous cohesion that packs an emotionally raw fervent punch.
Opening for such notables as X, Cyndi Lauper, and LA Guns, The Hail Marys were also featured in Override, a short narrative film by LIsa Ginsberg that played the International Film Festival Circuit and in Radical Harmonies, a Sundance Select 2003 documentary on the history of the women's music movement from 1970's to present day. Jackie, Debbie, Veronica, and Pyke never really broke up they just stopped playing together for a while as they ventured into parenthood, and sadly separate towns. When they resumed performing, each joined and/or created new projects and bands.


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"a masterpiece lost in plain sight"
There are currently three incredible productions of historic women's plays on stages across the country:
Funnyhouse of a Negro by Adrienne Kennedy at The Signature Theatre in New York,
The Sign in Sydney Brustein's Window by Lorraine Hansberry at the Goodman in Chicago, &
Trouble in Mind by Alice Childress at the Guthrie in Minneapolis.
"I bet you won't see anything so fearlessly weird and original all year. I don't know if Beyoncé is familiar with Kennedy's work, but Funnyhouse of a Negro plays like a hard-core retort to the self-empowerment poetics of Lemonade. That Funnyhouse of a Negro came a half century earlier hardly even matters" -- David Cote, Time Out NY
"[The Sign in Sydney Brustein's Window is] an extraordinary play, a drama so infused with emotional intelligence, linguistic treasures and the human conditions of dread and longing that it keeps you bolt-upright in your seat for nearly three hours... How can this play not be famous?" -- Chris Jones, The Chicago Tribune
"If a play as cogent, beautifully structured and up-to-the-minute as Trouble in Mind had been written by a white dude, it would have become part of the canon. But Trouble in Mind was written by a writer fighting two strikes — Alice Childress was black and a woman — and so it faded into obscurity." – Chris Hewitt, Twin Cities Pioneer Press


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Kansas City, KS 66111
Seeking a Full-Time Permanent Psychiatric Mental Health Nurse Practitioner to join an outpatient team!
Seeking a PMHNP in the Kansas City area to work primarily with the adult and geriatric population.This position is full-time outpatient and the hours will typically be Monday-Friday, 8a-5pm.
For consideration, candidates must be a graduate of an accredited PMHNP certification program. This position offers competitive compensation packages, Medical, Dental, and Vision, 401k participation, generous PTO, and ability to grow within the company.
This company is a full-service psychiatric treatment provider that offers solutions for various organizations. The providers develop individualized treatment methods to treat a full spectrum of psychiatric disorders for a wide variety of populations. Services are provided in psychiatric hospitals, residential treatment facilities, outpatient clinics, psychiatric consultations in hospitals, nursing homes, and FQHC's. The providers also utilize telepsychiatry in a variety of practice areas.
For more details please apply today!!
Please click APPLY ONLINE or email us a copy of your resume directly (my email is next to my profile). Be sure to check out all of our jobs at http://jobs.ka-recruiting.com. We look forward to working with you!
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Khan says new industrial measures needed
ENERGY Minister Franklin Khan said on Wednesday that new industrial relations measures must be adopted to ensure that the nation's industrial development thrust was not affected by labour unrest.
Khan was addressing a town meeting at the Vessigny Secondary School, La Brea.
He said although the aluminium smelter plant had been shelved by the previous People's Partnership administration, the downstream aluminium industry presented several opportunities for the region including the construction of a cable and wire plant and an aluminium flat roll plant. In a power point presentation, Khan said the region had had its fair share of "attempted industrialisation" opportunities and this was the third attempt to develop a Point Lisas type model.
"La Brea development has been characterised more by opportunities lost than opportunities found," Khan said, and noted that labour-related issues continue to hinder the region's development.
"TOFCO (Trinidad Offshore Fabricators Unlimited) had it when it was being constructed, TGU (Trinidad Generation Unlimited), had it when it was being constructed, CGCL (Caribbean Gas Chemical Limited) has it. We cannot continue like that," he said, adding, "Other jurisdictions have learned to resolve issues without obstructing development."
However he noted that he was not supportive of paying inferior wages or disregarding Occupational Safety & Health (OSH) standards.
"I do not support paying inferior wages. I do not support working under conditions that are contrary to OSH standards.
However, industrial relations resolutions needs to be adopted," he said.


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Christmas Impact Update is Here!
How do you impact children through GraceInside? Find out here!
Tevin tells his story of how he stole Christmas. We hear a quick word from Kate – and we find out what YOU are doing this Christmas behind bars.
Please take a peek at our Christmas Impact Update and find out what Jesus is doing in prison!


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Mode of Application
10th Mar, 2019 - 01st Aug, 2019
Babasaheb Bhimrao Ambedkar University (BBAU), Lucknow is a central university established in 1996, by Government of India. It is recognized by UGC and accredited as an "A" grade university by NAAC. The university has a dynamic campus with more than 4800 students enrolled in a broad range of graduate, post-graduate & doctorate programs.
BBAU offers UG programs in management, commerce, law, arts, education, and Library science. The minimum eligibility required for admission to the UG courses is a Higher Secondary degree in a relevant stream from a recognized board of education or university.
BBAU begins the application process for admission to UG courses in April every year. It is an online process and the application forms are made available on the official website of the university. The admission to these programs is through an entrance test conducted by the university. Check BBAU Admission 2019
The entrance test for each course is conducted separately. The test is conducted in offline mode around the month of May every year. After the entrance test, the university releases merit list of selected candidates who are then called for counselling/admission process.
10th Mar, 2019 - 01st Aug, 2019
|10th Jan - 15th May , 2019
|Date of Entrance Examination
|25th May - 10th Jun , 2019
|Announcement of Results
|01st Jul, 2019
|Counselling Session
|10th - 26th Jul, 2019
BBA (Bachelor of Business Administration) – 3 years (90 seats)
B.Com. (Bachelor of Commerce)- 3 years (120 seats)
BA Hons. (Public Administration)- 3 years (120 seats)
BBA -LL.B. - 5 years (60 seats)
Eligibility: Candidate should have passed 10+2 / intermediate with minimum 50% marks (45% for SC/ST/PH) from a recognized board/ equivalent.Bachelor of Science [B.Sc] + Master of Science [M.Sc] (Applied Geology) & Bachelor of Science [B.Sc] + Master of Science [M.Sc] (Basic Science)
Integrated B.Sc. - M.Sc. - 5 Years
Candidate must have passed Intermediate or 10+2 in the Science stream with 50% marks (45% for SC/ST/PH) from a recognized board/equivalent.Bachelor of Education [B.Ed]
B.Ed. (Bachelor of Education) - 2 years (100 seats)
B.Lib.I.Sciences (Bachelor of Library and Information Science)-1 year (40 seats)
Candidate should have a Graduation Degree in any discipline with minimum 50% marks (45% for SC/ST/PH) from any recognized University
Note: Candidates whose result is awaited are also eligible to apply, however such candidates have to submit the final result card at the time of admission.
The application process for BBAU is conducted in online mode. It is basically a five-step process. Candidates can apply for UG courses at BBAU by following the steps given below:
Step 1: Visit the official website: www.bbau.ac.in and click on "Online Admission System". You will be redirected to www.bbauesolutions.com.
Step 2: On the new page you will find the following information:
Step 3: To register, click on "Register and Apply" link. You will be required to enter the following details:
After filling the details mentioned above, you will need to review your form and submit it.
Step 4: In the next step, you need to upload the following documents. The file size should be maximum 80Kb and should be in jpg format.
Step 5: After uploading the documents, and submitting the application form, you will have to pay application fee. The application fee can be paid in both online and offline mode. Online payment can be done via Credit card/ Debit card/ Netbanking. Offline payment can be done via online generated challan at any ICICI bank branch.
BBAU conducts entrance exam for selection of candidates to UG courses. The entrance test is objective in nature. The university will prepare merit list on the basis of marks scored by candidates in the test. In case of equal marks in the entrance test, preference will be given to candidate with higher marks in the qualifying examination.
The course-wise selection process is given below.Bachelor of Business Administration [BBA] , Bachelor of Commerce [B.Com] {Hons.} & Bachelor of Business Administration + Bachelor of Laws [BBALLB]
The entrance exam for these courses will have question from the following topics:
In case of BBA LLB, there will be an additional section of Legal Aptitude in the question paper.Bachelor of Library and Information Science [B.Lib.I.Sc]
The entrance test for Bachelor of Library and Information Science will comprise of questions from the following topics:
|General Knowledge
|General English
|General Mathematics
|General Awareness
|Concept of Library and its services
|Awareness of Information Science and Technology
|National and International Famous authors and their books
|Knowledge about famous Libraries and Information Centers
The entrance test for B.Ed. will have questions from the following topics:
Integrated B.Sc. (Hons) – M.Sc. Applied Geology
The entrance test for this course will have questions on basic geology and general awareness.
Integrated B.Sc. (Hons) – M.Sc. Basic Science
The entrance test for this course will have questions from Biology, Chemistry, Physics, and Maths of 10+2 level.Bachelor of Arts [BA] {Hons.}
BA (Hons) Public Administration
The entrance exam for this course consists of two sections. Both sections will carry 50 marks each. Section one will have questions from General Knowledge. Section two will have questions from the following topics:
*The article might have information for the previous academic years, which will be updated soon subject to the notification issued by the University/College
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The Hearthstone Heritage wood burning stove features optional top or rear flue exits, greater heat output with a larger fire box, beautiful detailing, and soapstone facing on the side loading door. You can have your Heritage stove built with either a left or a right side loading door to accommodate your fuel storage option.
When fully loaded, the new Hearthstone Heritage can burn for up to 8 hours, providing up to 12 hours of sustained heat. The updated look of this whole house heater wood stove includes taller legs allowing for an ember protection only hearth requirement.
Right or left side load door
Oven style side load door
helps keep ashes in firebox
Top or rear flue exit
flexible installation
Single lever stove control
easy operation, consistent performance
Non-catalytic combustion system
clean burning, efficient, reduces creosote build-up
Removable ash tray
easy to clean, easily accessible


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Plan 2.2 Coursework and Research (BA>PhD)
Program structure
|Fundamental course
|LAAL700 Studies in Linguistics (3 credits)
For those who have never enrolled in Applied Linguistics as a subject (those who enroll with no credits)
|Core courses
|LAAL701 Seminar for PhD in Applied Linguistic Students (3 credits)
LAAL503 Writing for Academic Purposes (3 credits)
LAAL506 Principles in Language Pedagogy (3 credits)
LAAL507 Paradigms in Language Curriculum Development (3 credits)
LAAL710 Description of Languages and Language Use for Foreign Language Teaching (3 credits)
LAAL711 Advanced Essential Areas of Inquiry in Applied Linguistics (3 credits)
LAAL712 Design of Language Tests and Assessments (3 credits)
LAAL721 Advanced Research and Data Analysis in Applied Linguistics (3 credits)
|Elective courses
|elective 6 credits
LAAL750 Advanced Topics in SLA (3 credits)
LAAL751 Language, Culture, and Cognition (3 credits)
LAAL752 Pronunciation Fundamentals for L2 Teaching and Research (3 credits)
LAAL758 Independent Study (3 credits)
*students may take elective courses offered by the MA program in Applied Linguistics to fulfill this module.
|Minimum 48 credits
Admission requirements
a:Regular program
- Hold a BA in Linguistics, or related fields of study.
- Have a composite GPA of 3.50, or a second class honor or upper.
- Submit a PhD proposal.
- Fulfill the English proficiency requirements set by the Faculty of Graduate Studies.
|English language requirements
|IELTS: overall grade of more than 3 or
TOEFL iBT: overall grade of more than 32 or
TOEFL ITP held by Faculty of Graduate Studies, Mahidol University at a level of more than 400
|Admission numbers
|1/2561: 3 people
|Course duration
|4 years
|Coursework (credit)
|Thesis (credit)
|Min. GPA
|QF + FGS Requirements
|Fee (Thesis fee included)
*Currency: baht


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The purpose of a Gap Ministry is to provide a resource or services to meet ongoing needs that are not currently being met by other agencies or organizations. We currently work with our partner churches in the operation of Community Meals, a Clothes Closet, Little Luxuries, Bed Blessings & Beyond and a Laundry Ministry.
Lake Orion United Methodist Church
140 E. Flint St.
Lake Orion, MI
Immanuel Congregational Church
1 Hovey St.
Oxford, MI
Oxford United Methodist Church
21 E. Burdick
Oxford, MI
Christ the King Church
1500 W. Drahner Rd.
Oxford, MI
Tuesdays and Thursdays (closed Thursdays in June, July and August)
Christ the King Church
1500 W. Drahner Rd.
Oxford, MI
9:00 am - 12:00 pm
Donations of household and personal care items accepted
12:00 pm - 3:00 pm
Shopping Hours
Tuesdays and Thursdays
St. Mary's in the Hills Episcopal Church
2512 Joslyn Rd.
Lake Orion, MI
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Directed by Joe Hill-Gibbins. An Almeida Theatre production.
Within 2129 miles
is Carrera 1 No. 12 – 118, Cartagena
Directed by Joe Hill-Gibbins. An Almeida Theatre production.
Ian McKellen leads the cast of the five-star Chichester Festival Theatre production.
Presented by Sonia Friedman Productions and Fox Stage Productions
A co-production from The Old Vic and Headlong
Ralph Fiennes and Sophie Okonedo play Shakespeare's fated couple.
A co-production from National Theatre and Neal Street Productions
A production from Matthew Byam Shaw for Playful Productions, Robert Fox and Andy Harries.


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Month: March 2009
On Planning
"In preparing for battle I have always found that plans are useless, but planning is indispensable." Dwight David Eisenhower quote
This looks like a good tool
Virtual Certified Professional
Today I passed the VCP. Now onto the next challenge!
Always checking out new things. My friend Rob pointed me to this one. It makes firefox into an itouch. http://www.cooliris.com/
Trying xmind this week. It works with freemind but not personal brain. I will see how well it translates. Personal … More
Who is watching the people that write and produce movies today. I mean.. when are we going to get a … More
Fusion Logging for Windows
Windows Registry Editor Version 5.00 [HKEY_LOCAL_MACHINE\SOFTWARE\Microsoft\Fusion] "LogPath"="c:\\fusion" "EnableLog"=dword:00000001 "LogFailures"=dword:00000001 "ForceLog"=dword:00000001
http://www.wikihow.com/Stop-Being-a-Workaholic Thought this was an interesting "how to". I don't think I am a workaholic, but I can see where … More
Working on SOA
I am working on a presentation for the first SOA Symposium for the US DoD. This work is all about … More


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Today we will step outside of the box. Sure, there are dozens if not hundreds of software applications created specifically for keeping a personal journal or diary. But today, we will ignore that. We will imagine that we have to create our own personal, digital journal using other resources not specifically made for the purpose of journaling.
So, if you are feeling brave (I know I am), we will begin.
1. Video Journal/Log
You might think that you have a face for radio, but I'm here to tell you today that you have a face for a video journal. This simple way of recording and enriching your life experience is simple but often goes unnoticed. All you need is a webcam, smartphone, video camera, tablet or anything that can record your pretty mug. Spend a few minutes now and then talking into this recording device and over time you will begin to see how fulfilling this can become! Make a folder on your computer where you can store these video clips and name them by date. Password protection is also a nice edition if you really want to open up knowing that no one will see these videos.
Plus, Jake Sully did it on Avatar and we all know he is cool!
This video is for Skype interview tips but the same principles apply to a video journal or log.
2. Gmail Journal
As I discuss in Modern Journaling, simply using a journal account can be one of the cheapest and easiest ways to keep a personal journal. If you use a cloud service such as Gmail it is already password protected and secure and you can also include pictures and other media! Oh, and it's free!
Of course this just doesn't work with Gmail. Yahoo, Outlook and many other email clients are browser based (or at least have that option) and work the same was as Gmail. But seriously, what sounds better than Gmail Journal?
My advice would be to start an entirely new account for this purpose. The nice part about this is that you will now have a special email address that you can send or forward emails to and they will instantly become entries! This works nicely when your work blocks Gmail (like mine does). You can just send an email from your work account to this special Gmail Journal.
3. JPG Journal
Similar to the video log, this journal will essentially consist of a folder full of date-named files (.jpgs or picture files to be exact). This journal is a unique way to record your life by capturing as many parts of it as you can through pictures files. I say 'files' because it just doesn't have to be pictures of your life taken from your camera. Also include web clippings showing your blog, email, Facebook, daily news/weather and so on. You can also hand write entries or draw doodles and take pictures of these to include in this journal.
The end result will hopefully be a creative and varied reflection of your life. A benefit of this type of journal is that you can easily print out a picture book from a variety of online services which you could use to free your JPG journal from its binary prison.
4. Speak it Journal
Not only can you use your hands, fingers and face to keep a journal but your voice as well. This is often the best choice for those who spend a lot of time on the road or on walks.
There are actually two ways to do this: audio files and speech to text. The audio files method is simply recording your voice and saving it in a folder like the video journal and JPG journal in a format such as .wav or .mp3.
The speech to text method takes your spoken word and converts it into editable text using software. This can be accomplished using software on your Mac, PC or even mobile devices. Once it is in text you can throw it into a Word document or even journaling software such as Penzu or LDS Journal.
5. Touchscreen Journal
Yes, you will need a touchscreen for this. That's okay because most of us have these around these days including smartphones, tablets and all-in-one PCs.
For this type of journal you just need an application that allows you to draw or write on the touchscreen. Then you can hand write your entries using your finger or a stylus and add goodies such as doodles or drawings from your kids.
You can usually save these as picture files but it would be best if you can save them as a single PDF. This will allow the most portability and usability in the future.
This app is also a good place to start.
So, there you have it. Anyone else have a clever or unconventional way that you keep a journal or diary? Please let us know!


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I have a soft spot for artists who got their start at a young age. Let's just get that out of the way. From the Biebers to the Lovatos, I really struggle to critique anyone who had the responsibility of fame thrust upon them before they fully developed into a real person. 5 Seconds of Summer fall into that category. However, like a true professional, I am going to try my damnedest to push that aside for the band's third full-length studio release, Youngblood.
Unlike their previous albums, Youngblood doesn't attempt to claim the pop-punk label (and honestly, thank god because that was always a bit of a stretch). The band waved goodbye to the Madden brothers and the not-so-subtle "Sugar, We're Going Down"-sound-a-like riffs and dove headfirst into the world of pop.
While that change loses some of the band's boyish charm, it's a sure signal that the band has matured into a new stage of their lives.
Much of that maturation is shown off in frontman Luke Hemming's vocals which serve as a highlight of the album. Where melodies or lyrics (or other vocalists' vocals) may fall flat, Hemmings carries the extra weight. He gave us a taste of the 80s falsetto featured heavily throughout much of the record with the band's first singles "Youngblood" and "Want You Back".
"Valentine" will immediately remind listeners of 21 Pilots' "Stressed Out" and for good reason. Both tracks were produced by Mike Elizondo. The song's punchiness makes it a stand-out on the album, but not necessarily a good one. The track will likely polarize listeners, and it will be interesting to see the general consensus. For me, "Valentine" causes a viscerally anxious response I can't quite explain.
Tracks like "Talk Fast", "Moving Along" and "Better Man" offer a fun, peppy beat masking potentially heart-breaking lines like "Have you been eating breakfast alone like me?" and "Demons I tried to defend, but I couldn't get enough".
Is Youngblood revolutionary? No. Does it offer anything remotely new to the music world? Also no.
That being said, we should all give Youngblood a chance. Why, you may ask, should I waste my time listening to a seemingly subpar album that will undoubtedly be hailed as the second coming by thousands of 5SOS devotees? Let me tell you.
For a band who skyrocketed to unprecedented levels of fame after touring with One Direction and pushed out two albums practically within a year of each other, you have to applaud them for taking a risk with the album. While its contents may not be groundbreaking, Youngblood delivers something completely different from 5SOS's discography, and that takes guts.
Plus, I'm going to retract what I said in the opening paragraph – we have an obligation to look after our young musicians, musicians who (whether they wanted it or not) were forced into the spotlight before they could even legally drink and lost any sort of anonymity the majority of us rely on to get through our adolescent years.
In the age of social media, anyone (especially anyone with a blue checkmark next to their name) automatically becomes subject to truly insane amounts of attention on either end of the spectrum. On the one hand, you have a hoard of fans who would honest to god die for you. On the other, you have hundreds of people wishing you would just die. Both sides of the coin would be hard to manage, let alone having to do both simultaneously. The resulting effect can be an understandably isolating experience.
It's exactly that effect that much of the album speaks to. Lines throughout the record point to trying to drown out the loneliness and inner demons with partying and booze, but none do so as beautifully as the last track of the record. "Ghost of You" breaks your heart in a gorgeous way. Hemmings and bassist/vocalist Calum Hood lament a failed relationship and the utter loneliness that follows: "I chase it down with a shot of truth / Dancing through our house with the ghost of you". In fact, if you were still on the fence about listening to any of Youngblood, I implore you to at least listen to "Ghost of You". It's a sad but truthful way to end the album.
All in their early twenties, the Aussie band already feels the draining effects of fame. If we owe them anything, it's to see them through and to try our best to do better by our next generation of kids who just want to make music.
Recommended Tracks:
Want You Back
Lie to Me
Ghost of You
The 1975
Young the Giant


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Why You Can't Stop Checking Your Phone
Over the weekend I was at a housewarming with a few friends. At one point in the evening, two of us were called out for incessantly being on our phones. I hadn't realized how much time I spent on it over the course of the evening and mused that it isn't Apple's fault my iPhone battery dies so quickly when it was brought to my attention. In an effort to unplug and be present with our friends, we tossed our phones in the corner of the room. Being ten feet away from my phone felt uncomfortable, and I didn't even last an hour before picking it up again.
The physiological stress reaction in response to not being able to check a cell phone is something that affects many smartphone users. Constant use of smartphones hinders communication and signals rudeness is social setting, but this inability is psychologically based and can be downright dangerous in other settings.
The dangers of texting and driving have received a great deal of publicity and nearly everyone agrees it is hazardous. In fact, 94% of people believe it should be illegal, but 33% of American adults do it each month anyways. The numbers behind texting and driving speak for themselves. Texting while driving doubles the likelihood of getting into an accident and in 2011 alone texting caused 213,000 car crashes, 387,000 injuries, and 2,331 deaths. We know the dangers and think it should be outlawed, yet we're doing it anyways. Why?
When standing in line or waiting at a stop light, there is a seemingly physical urge to look at your smartphone. When your phone vibrates, it feels as if that text message is burning a hole in your pocket, compelling you to check it. Psychology tells us this is a conditioned stress based response. The notifications on a smartphone induce the physiological markers of stress and the only way to reduce that stress, urge, or anxiety is to check the phone.
Your phone goes off, you get the urge to check it, you check your phone, and then the urge goes away. If you think back to your introduction to psychology class, this is an example of operant conditioning, and more specifically, negative reinforcement. Negative reinforcement is the process of increasing the likelihood a behavior will occur by removing an unpleasant stimulus after the behavior occurs. In the example of your smartphone, you checking your smartphone (the behavior) removes the urge or stress (the unpleasant stimulus) so you're more likely to continue checking it in the future. You check your phone and bad feeling goes away so you keep checking it. It's a cyclical and cumulative process. This process of negative reinforcement is largely responsible for how the compulsions of Obsessive Compulsive Disorder (OCD) are developed, maintained, and strengthened. In many ways, attached smartphone users are similar to individuals with OCD.
As this pattern of negative reinforcement repeats, the responses are strengthened and become automatic. Like me at the party, many people respond to their phone without thinking or even realizing they are doing so. In other words, using your smartphone becomes an automatic process.
Automatic processing is the result of our brains creating shortcuts for things we do frequently, so we can save cognitive resources for more complex or important tasks. Your phone has programmed your brain to habitually check it, and to send out an unpleasant feeling when you don't. Recent research has shown people whose cellphone use is driven by such automated habits are more likely to text and drive. These cognitive processes are the reason people text and drive when they know they shouldn't or interact with their phone in the presence of others despite knowing they are being rude. Keep in mind, these phenomena do not justify such behavior, they merely explain it.
Psychology has not been as helpful at finding quick and simple ways to reduce phone checking compulsions as it has explaining it, but there are some in the works. I think exploratory interventions for texting and driving and should target the compulsory feelings that spur us to check our phones. I would consider applying treatments typically used for OCD like cognitive behavioral therapies (CBT). Prolonged Exposure is one of those treatments. To use PE, the individual would be exposed to that stressful feeling of knowing you have a text and not being able to check it for an extended period of time. By having this feeling, not being able to check it and then being shown that nothing bad happened from not checking it that stress response is lessened over time.
Systematic desensitization with reciprocal inhibition may also have merit as a treatment. That big technical name represents a treatment that is typically used to treat phobias. Essentially, the person is walked through a series of anxiety producing scenarios (in this case would include thinking you have an important text message, notification, or alert) while going through relaxation exercises. Pairing the stress inducing stimulus (thinking you have a text) with relaxation techniques teaches the body to automatically manage the stress of not checking a smartphone.
Using smartphones at inappropriate times can be explained by basic psychology, so interventions to reduce it should feature psychological methods. Shaming people, threatening them with jail time, or disabling their phones only addresses a symptom and ignores the root cause of the behavior. Even using behavior modification techniques, there is not a simple solution to reducing dependence on smartphones as everyone knows how hard it is to break a habit. Awareness is the first step to changing this behavior, especially since smartphone use has become an automatic process for many people. If you see someone glued to their phone at an inappropriate time, it is worth bringing it to their attention because like me at the party, they may not even realize they are doing it.
Follow me on Twitter @Aaron_Kraus


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Democratic challenger Alison Lundergan Grimes, who is trailing incumbent Senator Mitch McConnell (R-KY) in the highly competitive Kentucky U.S. Senate race, is claiming in a new television ad that her support for the Gang of Eight "immigration reform" legislation, which McConnell voted against, is "not amnesty." And she's relying on the words of Senators John McCain (R-AZ) and Lindsey Graham (R-SC) to drive that point home in the commercial.
Grimes herself is not heard in the commercial, which features only the voices of a narrator, McCain, and Graham.
"2013 will be the year, I hope, that we pass bi-partisan immigration reform," says Graham.
The unusual tactic of highlighting the words of prominent "pro-amnesty" Republican Senators, however, could well backfire. The ad, which aligns Grimes with two establishment Republicans utterly despised by grassroots Tea Party activists around the country, may, for the first time, give Kentucky voters who support the limited government principles of the Tea Party movement a legitimate reason to vote for McConnell over Grimes.
It was this core of supporters that gave Senator Rand Paul (R-KY) his upset victory in the 2010 Republican U.S. Senate primary and swept him to an easy general election victory that year. Until now, this voting block, which backed failed primary challenger Matt Bevin against the McConnell machine, had no reason to support McConnell over Grimes.
While Grimes' new ad is unlikely to gain any traction among a populace that largely opposes amnesty and is skeptical of claims by establishment Republicans and Democrats alike that the Gang of Eight's "path to citizenship" isn't really amnesty after all, the most engaged Tea Party activists in the state seem unlikely to see it as a reason to support McConnell.
Scott Hoffstra, a well-respected Tea Party activist in Kentucky, told Breitbart News on Wednesday, "This ad won't change the Tea Party's mind about McConnell. We know his true colors."
According to Hoffstra, McConnell "only voted no [on the Gang of Eight bill] because public opinion was against him. He put Rubio on the [Gang of Eight] panel to support amnesty and to give [himself] plausible deniability if it all went wrong."
"Republicans like McCain, Graham, and McConnell," Hoffstra added, "are pushing for amnesty to placate their Chamber of Commerce masters. Conservatives across the country threatening a revolt in November is the only thing that has prevented Boehner and McConnell from joining with Obama to push amnesty through. If McConnell wins reelection in November, he will be at the head of the line pushing for amnesty in the lame duck session."
Six weeks before the election, Grimes continues to trail McConnell by 4 to 6 points, depending upon the poll. The campaign has stumbled on its messaging and the handling of the invitation and subsequent disinvitation of billionaire Warren Buffett to a California fundraiser for Grimes last week.
Grimes' campaign looks rudderless and reactive and in need, as one Kentucky Democratic operative told The Hill on Wednesday, of a "shift [in] strategy."
"I genuinely believe, if it's an hourglass, the sands are running out [for the Grimes campaign]," Jimmy Cauley told The Hill.
"They've been outmaneuvered," University of Kentucky professor Al Cross told The Hill. "Most people had already made up their minds about Mitch McConnell, but she needed to make a case for herself. Contrasting with McConnell wasn't enough," Cross added.
The reactive nature of the campaign was highlighted by the fact that the new "This is Not Amnesty" ad from the Grimes campaign does not appear to be part of a strategic thrust but instead a response to an ad that a pro-McConnell Super PAC, the Kentucky Opportunity Coalition, ran last week, accusing her of favoring amnesty.
"Mitch McConnell voted against immigration reform that would have helped Kentucky farmers and secured our border by putting thousands more agents on the border. Alison opposes President Obama in any attempt to alter our immigration system by executive order and believes Congress needs to do its job and pass comprehensive immigration reform," Grimes campaign spokesperson Charly Norton said of the earlier ad.
Norton is also the spokesperson who claimed that the sponsor of the Grimes fundraiser in Northern California had invited Buffett to participate via conference call without authorization from the campaign.
Grimes's voice was not only missing from her new ad. It has apparently been missing on the ground in Kentucky for the past week, as she has toured the country on a fundraising tour replete with big liberal special interest groups. In addition to the mishap with Buffett, Grimes hit a Hollywood fundraiser hosted by film mogul David Geffen, followed by a scheduled appearance at a New York City fundraiser sponsored by the pro-abortion Emily's List. Though her name appeared on the invitation, at the last minute Grimes apparently thought better of showing up at an event sponsored by a group that supports a position that is very unpopular back home in Kentucky.
McConnell's constant negative attacks appear to be taking their toll on support for Grimes, just as they did on Bevin in the May primary.
And while many Tea Party activists appear unwilling to forgive and forget, the same may not be true for the less engaged supporters of Tea Party principles.
In a tight Senate race in a state that Barack Obama lost by 23% in 2012, McConnell could win without the support of the most engaged Tea Party activists. The support of some portion of the larger percentage of the population that simply considers itself supportive of the Tea Party's limited government message may be sufficient.
Among that group, as a recent poll by the Polling Company indicates, there is little support for the kind of "path to citizenship" Grimes, McCain, and Graham publicly support. And while Hoffstra's criticism that "the real McConnell supports amnesty" may well be true, those who do not follow the issues as closely as the highly engaged Tea Party activists may not draw that conclusion.


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XL Hollow Stainless Steel Urethral Insert
by: Master Series
$ 45.00
This smooth, super sized urethral plug has a hollow core and a ball capped end. The seamless shaft glides smoothly in and widens the further in you go, with a tapered shaft at the head to help keep it in place. The ball end keeps it from going in too far, and provides a visual adornment as you wear it. The hollow center allows for liquids to pass through, for long-term wear or creative play. The stainless steel material stays sparkling and bright, and cleans easily.
Measurements: 2.95 inches in total length, 2 insertable, 0.23 inches at base, 0.47 inches at widest point
Material: Stainless steel
Color: Grey


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Category: Creative
6 Ways to Have a Creative, Inspired Workday
Need to have a creative workday? Lucky for you, there are ways to make yourself more inclined to produce creative, inspired work.Read More
5 Things Quentin Tarantino Can Teach Us About Doing Better Creative Work
Quirky characters who ramble about anything and everything? Check. Ultra violence and a non-linear structure? Check and check. It's signatures like these that make Quentin Tarantino's movies stand out from the pack. Love them or hate them, you can't deny that they're unique. After a few decades of success, Tarantino has shared plenty of cool […]Read More