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Weekly Report Monday 6th May – Sunday 12th May 2019
Report by Andrew Tees
After the marathon heroics of Belfast, the majority of the club was taking a well earned break. However, the Orangegrove reporting team ensured that there was no shortage of material by enthusiastically (or dutifully?) toeing the line at various races around the province over the weekend. Many thanks to Gerald, Brian and Michael for their contributions to this week's report.
'Run the Runways'.
Newtownards Flying Club, Friday 10th May, 2019.
Report by Brian Todd
A number of OAC members gathered at a sunny Newtownards airfield for this unusual series of races hosted magnificently by the local running club, Scrabo Striders. There were a number of distances available, a 1k 'funrun', 5k and 10k. All the races were on the runways and aprons of the airfield. The wide main central runway has a number of shorter feeder runways and taxi areas. For the two longer distances all available runways were utilised to make up the distance with some sharp turns at each end. The 5k required one lap of the course and the 10k two laps. Inevitably, inclines were not an issue although the sharp turns required care and tended to eat into times. There was a slight breeze too which affected some parts of the course. The rather featureless aspect of the airfield was also a slight issue in that there were no distractions to take one's mind off the pain of trying to run as fast as possible. Nevertheless, conditions were perfect, dry and sunny, and it was a pleasure to explore the extremities of the airfield in this novel and innovative way. The 5k race was a round of the Baker-McKenzie Super 5s series so Brian Todd, the writer, plumped for the shorter distance as did Ross Robinson. Jenny Powell, Elaine Weston and the indomitable Michael O'Donoghue chose the longer distance. There was music round the course, the skirl of a lone piper and much food at the close of the running bit. All in all, a great evening, a memorable series of races and superb hospitality from the host club. The following results were achieved by OAC Members…
Ross Robinson…19.39
Brian Todd…20.19
Michael O'Donoghue….42.03
Jenny Powell…51.50
Elaine Weston…52.50
The 5k race was won by Gareth Lyons (North Belfast Harriers) in 16.12 and the first lady was Ann Terek (City of Lisburn AC) in 19.04.
The 10k race was won by Ross Moore (Saintfield Striders) in 36.23 and the first lady was Amanda Perry (Ballydrain Harriers) in 40.25.
Slieve Donard Race
Saturday 11th May
Surely one of the longest-standing races in the Northern Irish calendar, the Slieve Donard race took place for the 74th time on Saturday 11th May. The course has seen different variations over the years, but traditionally it has always started from outside the Newcastle Centre on Main St. From the start, the route led up the Glen River path to the first checkpoint at the saddle, then on to the main goal of Donard summit. From the top, runners had to negotiate the steep descent down the 'front side' of the mountain to the third checkpoint beside the quarry at the top of the Granite Trail. From there it was free reign to find the fastest route down through the forest to finish on the playing fields of Donard Park.
This year the race was not part of the NIMRA championship, but it was the first race in the Irish Championship, so a large number of IMRA runners had made the trip from various parts of the island (mainly Dublin) to compete. The weather was fine on the day, but wet conditions in the preceding weeks meant that the conditions were still boggy underfoot when off the main trail.
Newcastle AC's Seamus Lynch continued his dominance of Irish mountain running, winning the race in 56.50, almost 6 minutes ahead of second place. Esther Dickson of Newry AC was first lady, and 10th overall, in 1:05.42.
For Orangegrove, Andrew Tees finished in 1:07:01 for 17th/167.
Downpatrick Lions 3 Lakes Challenge 15K & 7K
11th May, 2019.
Report by Gerald Harvey.
In the 7K race, it was gradually uphill almost from the start to the left turn into a leafy minor road, then undulating through deepest countryside, until the right turn onto the Buck's Head Road hill. Unlike two years ago, I did not grind to a halt this time – honestly! The top of the hill is the highest point on the course (31m). With that little local difficulty overcome, it was an easy run to the halfway point and water station at the right turn into Drumcullan Road (the 15K race went straight on towards Loughinisland Lake). Getting some water helped and it continued to be an easy run for the next 2K, passing the northern tip of Magheralagan Lake where a really nice house sits close to the water. After the church in the last 1.5K, there is an uphill drag that hurts a lot more in the longer race. The hard work is done when you see Ballydugan Lake through the trees on the old railway embankment, prompting a downhill sprint to the finish in the car park opposite the quaint Lakeside Inn.
Downpatrick Lions Club and Atlas Running organized the event with proceeds in aid of Mainstay DRP, a local charity that provides supported living and respite care for people with learning disabilities. ChampionChip Ireland did the chip timing. The marshals were very good and a reassuring presence all around the course. The PSNI were out on the roads as well. Unfortunately only 114 runners in total showed up this year for the two races. Come on Orangegrove AC, we should be doing this!
The 7K race was won by Nigel Greer (Dromore AC) in 26:39 and first lady was Lorna Chambers (Murlough AC) in 31:08, 5th out of 51 runners. Gerald Harvey (Orangegrove AC) was 15th in 35:00.
The 15K race was won by Gareth Lyons (North Belfast Harriers) in 52:44 and first lady was Roberta Dornan (North Down AC) in 1:08:02, 11th out of 63 runners.
Carrickfergus Marathon
Saturday 11th May
Report by Michael O'Donoghue
We Run Wild is a new local organisation that aims to offer something a bit different for local runners. It takes some ideas that have been tried elsewhere that the organisers, who have done a lot of ultras, have found adds to the experience. I first heard about their ventures when they announced "On the Run from the Crum" a 24 hour self-navigating race which is based upon an English event, Escape From Meridan.
The Carrickfergus Marathon on Saturday 11th May was their first event. The idea that made it different from other local events is a "road book" where instead of a marked course, or map, you'd have written directions at each junction indicating the way to go. The route started in Carrick Castle before climbing up through minor trails all the way up into the hills towards Woodburn Forest and Reservoir before heading back down.
It was an unusually hilly course for an event that starts in an urban setting, totalling over 2000 feet of climbing. Much of it was trail though, and it made extensive use of Woodburn forest, meaning that overall it was as much trail as a number of events in The Mournes. It was a mile long, which nobody seemed to mind. After Belfast last week everyone is an ultra runner now! It would have been very difficult to mark an accurately measured course on that terrain in any case!
It was a familiar sight at the start of the race, with many curious ultra runners from all over Ireland and England. The winner was Bobbie Irvine whose time was 3.56.49. First lady was Fiona Prue (4.43.51) My time was 5.46.27
Consolidated club report here: https://www.parkrun.com/results/consolidatedclub/…


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The medical career is an incredibly complex and fascinating one that provides many unique and exciting facets that help to offer hard workers a robust financial situation. However, it is also true that medical careers are often incredibly diverse and provide many benefits. Unfortunately, many people have a hard time knowing what kind of career makes the most sense for their needs.
So, if you're still not sure how to answer the question 'what career should I choose in the medical field?' you should consider the options below to get a better idea of which is right for you. We'll also talk about a few side hustles that might be right for many people in your situation. In this way, you should be able to get the fantastic career that you want and feel comfortable at the same time.
Physical Therapy
When trying to answer the question, "what career should I choose in the medical field?" it is crucial to take the time to consider physical therapy seriously. This career option is hugely beneficial for many reasons, each of which is important to think seriously before starting your new career.
For example, there is a massive demand for physical therapist professionals who can help handle the various difficulties that various types of injuries and other health issues might cause. These experts fully understand these unique opportunities and help to keep their clients as strong as possible.
And therapists of this type often can work either independently of hospitals or other care centers or can also get hired directly by these teams. The option will vary according to their needs and provides many unique possibilities that they can consider for their new medical career path.
Thankfully, the high demand (and likely increasing need) for this career field will make it reasonably easy for most people to get started in this career field. So, don't forget to check out the different options available for your needs as a person to ensure that you're satisfied.
Urgent Care Worker
While you might not consider working in an urgent care facility when deciding "what career should I choose in the medical field?" you may want to do so. Why is that the case? Urgent care centers are almost always in high demand for doctors or nurses who can work in what can be difficult situations.
However, urgent care workers often have higher pay and better amenities than many other nurses because their careers are demanding. Just as importantly, you will be in what can be a fascinating position, with many new patients and experiences occurring every day.
For example, an urgent care center is often a lot like an emergency room with a very high-energy and exciting experience with people coming and going with things like severe cuts, flu-like symptoms, and much more. Few medical jobs are as hands-on and as busy as urgent care.
And if you work hard enough, you might even be able to open up an urgent care clinic yourself, making even more money. This option is a great career goal but requires you to understand things like how much money it costs to open up a new facility and what you need to do to cut back on financial struggles.
Even better, you can create a whole series of clinics that help to serve your community or the surrounding area. That type of comprehensive care is a wise choice because it helps create a unique level of treatment therapy that will make your community safer and your career stronger.
CNA Career
Many people asking, "what career should I choose in the medical field?" may ultimately base their decision on the kind of training they have to do. But, on the other hand, some people may want just to get started on their career without having to spend the rest of their life training for a great job.
That's why many people consider a CNA job as a great way to break into the medical field. This option allows you to become a medical assistant for nurses and break into what can be a very challenging field without training nearly as long as you would have to for a nursing profession.
Just as importantly, there's a massive demand for high-quality nursing assistants. It is true that you'll be doing a lot of the harder or even dirtier work that other nurses don't want to do. Expect to be working a lot during your shift and to feel a bit tired at the end of every day.
However, the right person will enjoy that feeling of accomplishment as a person. Just as importantly, you can often get high-quality training that makes it easier to transition to a new career. For example, many hospitals will pay to get you trained to a whole nurse level to make your job easier.
So if you're considering "what career should I choose in the medical field?" and aren't sure that you are ready for a lot of training, it might be a good idea to become a CNA. In this way, you can cut back on the training time that you need and ensure that you are satisfied with your career options.
Dental Professional
People wondering, "what career should I choose in the medical field?" may scoff at the idea of becoming a dentist. The old joke states that a dentist is a failed doctor. However, this gag is very unfair. A great dental professional is a specialized medical professional who is vital for many care needs.
Opening up a dental practice has many apparent benefits that cannot be ignored if you're interested in a medical field. First, this option helps make it easier for you to get through training, as you usually don't have to go to dental school anywhere near as long as you do for other medical career options.
Just as importantly, there is a high demand for dentists in just about every town. Not every medical professional (including surgeons) has the unique skill set necessary to be a dentist, meaning that you can find a surprising range of potential career options in this field.
Even better, you may be able to start out working as an assistant or as a dental cleaner in an established facility, make connections with potential clients, and then use them to create a new practice later. But, again, that kind of standard practice is not uncommon in what can be a pretty competitive field.
Many dental professionals even create multiple practice centers throughout their care center to create a unique level of career options. So, if you want an independent medical career that could be very lucrative, it is a good idea to get trained as a dental professional.
Health Care Planner
If you're not much of a hands-on person but still want to know "what career should I choose in the medical field?" it might be a good idea to consider a career in medical planning. This option has become famous for many reasons and could be suitable for the right person.
For instance, if you're someone who has great ideas of what makes high-quality medical care, but you don't like working with your hands, this option is excellent for you. It will allow you to use your brain and not your hands. And it also gives you a chance to work independently of other medical teams.
For example, you may need to rent out office space to start planning for various types of medical care options. As a planner, you also need to make sure that you find clients who need your services, including multiple types of hospitals and professionals who understand your needs.
You may need to understand things like advanced health care directives that direct treatment options and help to ensure that things go as smoothly as possible. In this way, you can give yourself the best chance of thriving in what can be a unique and sometimes challenging field for many professionals.
Great Side Hustle Careers
If you're having a hard time answering the question 'what career should I choose in the medical field?' even after reading about these options, you may also want to consider the benefit of a side hustle. This unique career option has become very common with a variety of different people over the years.
What is a side hustle? It is a side job that you can do outside your standard career field. And even medical professionals as diverse as surgeons are turning to fun and unique side hustles as a way of preparing for their retirement or expanding their potential earning possibilities as a person.
How do you choose a side hustle? First, it is essential to find something that you can do on your schedule, working in and out of the sometimes hectic work demands of the average medical professional. Just as importantly, you need to find something that you enjoy doing. Just a few options include:
- Creating Great Art Work – If you have any artistic skill, you can create fantastic and unique pieces that will capture the imagination of many people and interest them in your field. For instance, you can buy a large ice block and carve individual decorative items that last a short time but provide a fascinating look.
- Gold Trading – These days, the gold trade has become huge in many circles. For instance, you could become a gold buyer by seeking out computer parts, pulling out the golden materials, and selling them to other people at a reasonable price. And you buy gold on your off-time and days off.
- Jewelry Dealer – Did you know that there's a huge market for high-quality jewelry sold at reasonable and respectable prices? If you go to various thrift shops or talk to your family members and buy jewelry, you can find a jewelry buyer who may help you make good cash in this unique way.
- Other Types of Buying and Trading Gigs – If you seek out inexpensive and high-quality items, you can usually sell them for surprising amounts of money. For instance, you can work with a gun shop to buy and trade various guns and make a decent and incredible amount of money at the same time.
Even if you feel comfortable answering the question 'what career should I choose in the medical field?' and know what kind of training you want here, a good side hustle is still a great option because it can provide you with a surprising array of benefits that you might not expect.
For example, it can give you an excellent hobby that helps to make your life a bit more enjoyable in many ways. Even better, it can help break apart the difficulties of the medical profession and give you a fun outlet for many creative and exciting hobbies and career choices.
Just as importantly, you may want to master a side hustle as a way of preparing for your medical career. For example, any of the options above could help you pay your way through medical school. That benefit is huge because it will help cut back on how much money you owe in student loans.
In this way, you can get started on your medical career without having to worry about struggling to pay your bills. And these side hustles may also help show you what kind of things you enjoy doing as a person. In this way, it should be easier to identify the best career options for your needs and cut back on your confusion.
So, do you feel comfortable knowing the answer to the question, 'what career should I choose in the medical field?' If not, it is vital to seek more answers from high-quality career training professionals who can help you understand what options are suitable for your needs. In this way, you can expand your potential options and feel comfortable getting the best career for your needs as a person.

subset: commoncrawl


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After my Graduation, it was over a cup of Coffee that my friend introduced me to Digital Marketing and It was love at first sight. With an experience of more than 2 years in digital marketing, I now want to share my experience and knowledge with the Odyssey Community.
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In a sector worth £184 billion, the technology space is quickly becoming one of the most exciting industries to be a part of. In fact, it is expanding 2.6 times faster than the rest of the UK economy and with each new day, a wealth of new companies joins the fold.
It is a competitive space and customers are now having to navigate their way through a crowded marketplace of products and services. For businesses, identifying prospective customers and securing down a sale is often impossible without the assistance of a network of channel partners.
Tell me more about the channel, what is it and how does it work?
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Whilst the channel offers a platform to extend the reach of a product or service, it is not an automatic process, and it does require work. For vendors, the perceived difficulty with working in the channel can be the lack of results or complexity. But often it's down to reduced investment – whether this be in people, tools or wider processes.
Identify and work with the right people
Partner engagement and enthusiasm to sell is a critical part of channel marketing. For the vendor, the focus should not just be on acquiring new partnerships but nurturing (and ultimately growing) existing relationships. A clear line of communication and easy access to support and assistance where required is key to this relationship, as is listening to opportunities to develop the partner offering further.
To maintain engagement, measurement of campaign performance is also critical here. The vendor can best determine where the spend should be allocated (and to which partner), based on the results achieved, but it can also prove useful in highlighting the engaged partners (and ensuring they are rewarded accordingly for their efforts).
Invest in the most effective tools
In channel marketing, streamlining messaging, ensuring consistent content and tone of voice, and keeping assets all in one centralised location helps vendors to ensure their offering remains aligned and competitive.
Critically, the perception of the vendor brand is ultimately in the hands of its partners – the way that channel partners talk about or sell the product or service needs to be clearly defined and managed from the offset – and the opportunity to edit any of this with a partner's own branding or positioning needs to be minimised.
When it comes to co-branding, consistency is particularly important. It is here, that there is the most risk of your messaging being lost. A centralised hub of content and communications can essentially serve as a toolkit for both the vendor and its partners to use across the channel – ensuring the product or service messaging is not only being amplified but sold well and not lost in the landscape.
Investing in your processes
When it comes to channel marketing, collaboration is key to success. It is key to choose potential partnerships wisely; a thorough analysis should always be undertaken to determine whether the vendor's product or service will be a good fit. This analysis should consider the existing product offering of the partner, and the audience which they sell to.
That said, the relationship is two way. For partners, vendors must be prepared to answer, "what is in it for me?" Recent data from Canalys indicates that partners consider partner programmes to be less relevant and fit for purpose than they used. The vendor must be able to provide the education, support and materials the partner will need to drive a sale to solidify a mutually beneficial relationship.
Many organisations struggle to make the most of their channel marketing due to limited resource, lack of direction or outdated tools and applications but with the appropriate investment in time and material, the rewards can be great.
Whether you are looking to enhance your existing partner programme, develop a new strategy or execute impactful collateral, get in touch. We have a wealth of experience in this space and have developed offerings which include all you need to deliver a successful channel marketing strategy. Find out how, and speak to us today, at firstname.lastname@example.com.


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text:
- Research article
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The application research of xTAG GPP multiplex PCR in the diagnosis of persistent and chronic diarrhea in children
BMC Pediatrics volume 20, Article number: 309 (2020)
Persistent and chronic diarrhea is difficult to treat, and infection is still the main cause. In this study, we investigate the application value of xTAG gastrointestinal pathogen panel (xTAG GPP) multiplex PCR in the early diagnosis of persistent and chronic diarrhea in children and to understand the epidemiology of intestinal diarrhea pathogens.
One hundred ninety-nine specimens were collected from Nanjing Children's Hospital Affiliated to Nanjing Medical University (Nanjing, China). We compared the xTAG GPP multiplex PCR assay with traditional methods (culture, rapid enzyme immunoassay chromatography, and microscopic examination) and performed a statistical analysis.
The positive rate of the xTAG GPP multiplex PCR assay of diarrhea specimens from 199 patients was 72.86% (145/199). The virus detection rate was 48.7%, and rotavirus A was the most common organism detected (34.67%), concentrated in winter, and was common in children. The second most common organism detected was norovirus GI/GII (20.6%). The positive rate of this bacteria was 40.2%, and Campylobacter (22.11%, 44/199) was most frequently detected. C. difficile toxins A/B and Salmonella was detected in 44 and 17 samples, respectively. Infections with Shigella occurred 4 times, and E. coli O157 was only detected once. Three samples were parasitic (1.51%), two samples were positive for Entamoeba histolytica, and one was positive for Cryptosporidium. Adenovirus 40/41, STEC, ETEC, Giardia, Yersinia enterocolitica and Vibrio cholerae were not detected. In total, 86 (43.2%) infected specimens with a single pathogen were detected. There were 59 coinfections (29.65% of the samples) of viruses and/or bacteria and/or parasites. Coinfections involved 49 double infections (24.62%), 9 triple infections (4.52%) and 1 quadruple infections (0.5%). Norovirus GI/GII was found to have the highest involvement, with 32 coinfections (16.08%).
The xTAG GPP multiplex PCR assay is simple, sensitive, and specific and can be used as a quick way to diagnose persistent and chronic diarrhea in children.
Diarrhea continues to be a health burden worldwide, especially in children living in developing countries. It is estimated that in these regions, it is responsible for 2.5 million infant deaths annually, with a mortality rate of 4.9 per 1000 children and an annual incidence of 3 episodes per child among children under 5 years of age [1, 2]. Most of the diarrheal illnesses are acute, lasting no more than 7 days; however, approximately 3–19% of the acute episodes last more than two weeks, which is called persistent and chronic diarrhea [3]. Persistent and chronic diarrhea cases are difficult to treat and their treatment cost is higher, and a case fatality rate as high as 60% has been reported [4]. It is more important to determine the cause of chronic diarrhea by a systematic approach because it can provide the most suitable therapy and give a good prognosis. The causes of chronic diarrhea are divided into infectious and noninfectious etiologies. In developed countries, the incidence of noninfectious-based diseases (food allergies, enteropathy or inflammation) is increasing. However, in developing and industrialized countries, the most common and most important cause of persistent and chronic diarrhea is still enteric infection [5, 6]. It is crucial for timely and effective treatment of infectious diarrhea in the rapid identification of pathogens because appropriate antimicrobial therapy and/or isolation measures to prevent the spread of infectious agents to healthy people can shorten the disease and reduce some bacteria and parasite infection incidence and can help reduce invasive infections [7, 8]. The gold standard for the diagnosis of infectious chronic diarrhea pathogens is culturing pathogens, but this method takes a long time (72 h) and requires more fecal sample [9]. In recent years, the development of faster and more sensitive molecular tests that can detect various pathogenic agents of bacteria, viruses and parasites might improve the etiological diagnosis of diarrhea pathogens [9,10,11].
The Luminex® Corporation has developed a new qualitative bead-based multiplexed molecular diagnostic test, the xTAG gastrointestinal pathogen panel (xTAG GPP), that can be performed directly on stool samples to detect and identify 15 pathogens in a timely manner: Adenovirus 40/41, Campylobacter, Clostridium difficile, Cryptosporidium, Entamoeba histolytica, enterotoxigenic Escherichia coli (ETEC), E. coli O157, Shiga-like toxin-producing E. coli (STEC), Shigella, Salmonella, Giardia, norovirus GI/GII, rotavirus A, Vibrio cholerae and Yersinia enterocolitica [12]. The clinical manifestation of xTAG GPP was recently evaluated in many infectious gastroenteritis cases, the sensitivity and specificity is better in xTAG GPP than in traditional methods [13, 14].
Thus, the purpose of our study was to explore the distribution of enteropathogens in patients with persistent and chronic diarrhea in Nanjing, China and to further evaluate the performance and applicability of xTAG GPP in identifying pathogens in these children.
Sample collection
A total of 199 stool samples were prospectively collected from 199 diarrheic children mainly under 5 years of age (85.93%, Table 1), including 109 simple diarrhea and 90 secondary diarrhea (colitis, pneumonia and tumor-associated) patients who attended the Nanjing Children's Hospital Affiliated to Nanjing Medical University (Nanjing, China). The study protocol was approved by the ethics committee of the Children's Hospital of Nanjing Medical University (Nanjing, China). Written informed consent was obtained from the proband and their parents. One sample was received from each patient. Inclusion criteria: patients with diarrhea that presented as watery and/or loose and/or mucous and/or blood stools with ≥3 instances within a 24-h period. Patients with inflammatory bowel diseases were excluded from the study. Stool samples were sent to the Department of Microbiology for investigation. Five grams of fresh stool samples were collected into empty tubes and placed in Cary-Blair Transport Medium for bacterial culture. Stool specimens were then stored at − 80 °C until processing with multiplex PCR tests. Unqualified samples (sample volume < 5 g, swabs not preserved in Cary-Blair Transport Medium) were rejected, and resubmission was requested.
Routine diagnostic methods
Stool culture for Salmonella and Shigella was performed using Salmonella–Shigella agar plates and Hektoen enteric agar plates. To detect toxigenic Clostridium difficile A and B toxins and norovirus GI/GII real-time reverse transcription-polymerase chain reaction (RT-PCR) assays were performed on the 7500 real-time PCR platform (Applied Biosystems, Foster City, CA). Rotavirus was detected directly in stool samples with the Diagnostic Kit for Rotavirus, rapid enzyme-linked immunosorbent assay (ELISA) tests. All assays were carried out in accordance with their respective instructions. We looked for Entamoeba histolytica and Giardia lamblia by microscopic examination of fresh stools.
Multiplex PCR and molecular diagnostic assays for the detection of 15 pathogens
Total nucleic acids were extracted from the stool samples using the NucleoSpin® Virus Kit (MACHEREY-NAGEL, Germany) according to the manufacturer's instructions. An internal control (bacteriophage MS2) was included in each specimen to control the quality of the detection process. The RT-PCR experiments and subsequent hybridization steps were performed according to the instructions in the xTAG GPP manual. Negative and positive controls were included in all runs of the xTAG GPP assay. The data were acquired on the Luminex 200 analyzer, and data analysis was carried out using TDAS GPP version 1.11 (xTAG Data Analysis Software).
Demographic and clinical parameters of patients with persistent and chronic diarrhea
The demographic and clinical characteristics of the 199 patients are summarized in Table 1. One hundred ninety-nine stool samples were prospectively collected from 199 diarrheic children under 5 years of age (85.93%, 171/199), with a mean age of 12.93 ± 15.86 months. The percentage of boys (58.29%, 116/199) was slightly higher than that of girls (41.71%, 83/199). There were 163 persistent cases and 28 chronic cases of diarrhea. The majority were inpatients (88.44%, 176/199) during the study period, and no deaths were reported. Of the 199 stool specimens submitted to laboratories, watery/loose stool (n = 139, 72.78%) was the most common type, and mucus/bloody stool was less than 30.15% (60/199).
Pathogens detected with the xTAG GPP
In this study, we found that 145 (72.86%) of the collected 199 samples had positive results. Of these, 97 samples were positive for viruses, with rotavirus A being the most common organism detected (34.67%; 69/199). The second most abundant virus was norovirus GI/GII, which was detected in 41 patients (20.6%; 41/199). Bacterial pathogens accounted for 40.2% (80/199) of all enteropathogens; Campylobacter (22.11%, 44/199) was most frequently detected, and C. difficile toxins A/B and Salmonella were detected in 44 and 17 samples, respectively. Infections with Shigella occurred 4 times, and E. coli O157 was only detected once. There were three parasitic samples (1.51%); two samples were positive for Entamoeba histolytica, and one was positive for Cryptosporidium. Adenovirus 40/41, STEC, ETEC, Giardia, Yersinia enterocolitica and Vibrio cholerae were not detected. There were 59 coinfections (29.65% of samples) of viruses and/or bacteria and/or parasites (Table 2). Coinfections involved 49 double infections (24.62%), 9 triple infections (4.52%) and 1 quadruple infections (0.5%). Norovirus GI/GII was found to have the highest involvement in coinfections 32 (16.08%), followed by rotavirus A (15.58%, 31/199), Campylobacter (12.56%, 25/199) and C. difficile toxin A/B (10.05%, 20/199) (Table 3).
Comparison of the xTAG GPP and conventional detection methods
Among the enteropathogens that could be detected by xTAG GPP, 5 enteropathogens (STEC, ETEC, adenovirus 40/41, Yersinia enterocolitica and Campylobacter) could not be detected by routine detection methods; therefore, in this study, the specificity and sensitivity of this method for the diagnosis of these five enteropathogens were not compared. As shown in Table 4, the sensitivity was 100% for norovirus GI/GII, C. difficile toxin B and Shigella, 96.9% for rotavirus A and 33.3% for Salmonella. The specificity was 100% for all targets except Entamoeba histolytica (99.5%), E. coli O157 (99.0%), Cryptosporidium (99.0%), Shigella (98.0%), Salmonella (92.3%), rotavirus A (89.3%), norovirus GII (89.3%) and C. difficile toxin A/B (84.9%). Among the 10 comparable enteropathogens, 2 enteropathogens (Giardia and Vibrio cholerae) were not detected in our samples by either xTAG GPP or routine assays, so it is impossible to evaluate the sensitivity of these enteropathogens. The overall sensitivity and specificity of xTAG GPP for the diagnosis of intestinal pathogens were 96.3 and 98.2%, respectively, which were significantly higher than those of conventional detection methods. The sensitivity and specificity of this method to individual pathogens are shown in Table 4.
Age and sex distribution of children with enteropathogens
The prevalence of enteropathogens among sex groups was compared, 88 (75.86%) male patients and 57 (68.67%) female patients were positive for enteropathogens. The distribution of enteropathogens was similar in both boys and girls (Table 5), with rotavirus A being the most common pathogen detected at 39.66 and 27.71%, respectively, followed by Campylobacter and norovirus GI/GII, and there was also no significance in coinfection (p > 0.05). The distributions of viruses, parasites and coinfections were similar in the three age groups (0–12 months, 12–60 months and ≥ 60 months), with P values of 0.73, 0.724 and 0.76, respectively (Table 5). Rotavirus A was the most common enteropathogen in patients 0–12 months (37.9%) and 12–60 months (33.33%), while Campylobacter was the most frequent enteropathogen in patients ≥60 months (28.6%, 8/18). In this study, bacterial infections were the most common in the 12–60 months age group (57.1%) compared with the other age groups (33.3–46.4%).
Seasonal distribution of children with enteropathogens
In this study, the seasonal curve of viral infection had a peak in the winter and a trough in the summer. Rotavirus A was the most important enteropathogen, and the infection peak occurred from November 2014 to February 2015 (Fig. 1), with the highest proportion occurring in December 2014 (90.0%, 18/20). In contrast, bacterial agents had a peak in the summer and a trough in the winter, Campylobacter was the most frequent enteropathogen, and the highest proportion occurred in October 2015 (75.0%, 15/20).
There are few data that simultaneously describe the prevalence of bacterial and viral pathogens in children with persistent and chronic diarrhea in China. In our research, persistent and chronic diarrhea were related to sex and age and commonly existed in boys, especially in children under 2 years old. In our study, 129 patients ages 0–1 years old (64.82%), this was similar to the research showing that the morbidity age was 4 months to 1 year [3]. Patients who had watery and/or loose stool predominated more than patients who had mucoid/bloody stool, even though most samples were collected in the winter.
In this study, the overall sensitivity and specificity of xTAG GPP were 96.3 and 98.2%, respectively, which were more efficient than routine detection methods. At the same time, there were significant differences in single or mixed intestinal enteropathogen infection (P < 0.001) (Table 4). In the present study, the xTAG GPP method efficiently detected infection in approximately 59 (29.65%) out of 199 children and showed multiple positive results (coinfection); this figure is higher than the positive result, which was relatively high and also detected by xTAG GPP, in a previous study by Deng J et al. [13]. Norovirus GI/GII was found to have the highest involvement in coinfections in our study.
In our study, rotavirus A was the most common pathogen in children with chronic diarrhea in the spring and autumn, followed by norovirus. Previous reports have shown that rotavirus A is the most common virus that causes diarrhea in children [15]. Moreover, norovirus is an important cause of diarrhea in adults and children [16]. This result was similar to other studies conducted previously in China [17,18,19] as well as other countries prior to the introduction of rotavirus vaccination [20, 21].
In our study, 4 samples with a positive Salmonella culture had no positive results in xTAG GPP, indicating false-negative results. This phenomenon is consistent with some previously reported results [13, 22]. The cause of the failure of the Salmonella pathogen requires further sequence analysis or qPCR assay investigation. Special attention should be paid to the occurrence of Campylobacter because the detection of this pathogen is rarely requested in patients with diarrhea; however, in our study, we detected a high frequency of 25% (43/199) for Campylobacter.
In conclusion, our research shows that xTAG GPP has very good sensitivity and specificity in detecting pathogens associated with persistent and chronic diarrhea. This method can shorten the detection time and reduce false-negative diagnostics, identify the cause of infection more quickly and accurately, provide a basis for accurate follow-up clinical treatment and improve the prognosis of the disease. However, the number of samples in this experiment is limited, and some pathogens have no positive samples (Giardia and Vibrio cholerae), so it is impossible to compare the results from different methods.
Availability of data and materials
All data generated or analysed during this study are included in this published article.
- xTAG GPP:
xTAG gastrointestinal pathogen panel
Enterotoxigenic Escherichia coli
Shiga-like Toxin producing E.coli
Realtime reverse transcription-polymerase chain reaction
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This work was supported by the National Natural Science Foundation of China (grant number: 81570470). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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CLW and XYZ researched the topic, analyzed the data, and was a major contributor in writing the manuscript. MSZ and XYW collected clinical stool samples and clinical data. HJY, YH and JMT completed relevant experiments and collated experimental data. ZFL, YJ and BXZ guided writing and critically reviewed the manuscript. All authors read and approved the final manuscript.
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The study protocol was approved by the IEC of the Children's Hospital of Nanjing Medical University (Nanjing, China) and approval number is 201901013–1. Informed consent, additional clinical information and stool samples were obtained from all subjects. The study protocol was approved by the ethics committee of the Children's Hospital of Nanjing Medical University (Nanjing, China). Written informed consent was obtained from the proband and their parents.
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Wang, C., Zhou, X., Zhu, M. et al. The application research of xTAG GPP multiplex PCR in the diagnosis of persistent and chronic diarrhea in children. BMC Pediatr 20, 309 (2020). https://doi.org/10.1186/s12887-020-02206-6
DOI: https://doi.org/10.1186/s12887-020-02206-6


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It is one thing, and fairly incorrect, to label a game in September or October as one that carries "great urgency" or one that indicates a "litmus test" of some sort for an NFL team. We tried that in Week 2 when the Eagles played Dallas and, in a narrow loss, provided the encouragement and the belief that the Eagles could play with any team in the league.
We're way past that now. Moral victories, never of import to the team, mean a whole lot of nothing in the big picture, anyway. As the Eagles line up for this keep-the-season-alive game against New York, everything is on the line. The Eagles need a win to keep their playoff hopes alive. They need to win in the NFC East. They need to go on the road and beat an 11-1 team.
So what is this game going to tell us about your favorite football team? There are a bunch of things on the hook ...
**1. Can The Eagles Run the Football Against A Top Defense?
You know about the weather forecast. And you know that Nick Cole is starting at right guard. And you know the Giants are very good along their front seven. What we don't know is how much Andy Reid wants to run the football, and how he intends to scheme the run game. Kyle Eckel is expected to be active to get some playing time.
Are the Eagles going to come out running? What are the plans with the short-yardage approach? Do the Eagles tempt the edge, or are they going to go directly at the Giants? What about the offensive line, which reacted to criticism last week with a tremendous performance against Arizona?
Brian Westbrook needs to have a big game here. The Giants are going to key on him, of course, and the Eagles must work to get him in space. If Westbrook comes anywhere close to what he did against the Cardinals ...
**2. Is This Offense Ready To Start Quickly On The Road?
Here we go again. The Eagles are away from home and in recent outings away from the friendly confines the offense has struggled. The woes in Cincinnati and Baltimore are in the past, however. Donovan McNabb hopes to ride the momentum created last week and start well against a Giants defense against which he tossed three TD passes one month ago.
Everybody wants to know what the Eagles plan to do from a scheme standpoint. McNabb has to be good for the Eagles to win, because the Eagles are going to have to make some big plays in the passing game to score points. Nick Cole is a key up front, but the entire offensive line is on the spot here.
Big game. Big moment in the season and, truthfully, for the era here.
**3. Can The Run Defense Rebound?
Tough game last time around. You would think the Eagles will draw upon all of their pride and emotion and energy to improve on their run defense in this game. How much Jim Johnson's scheme changes is something to watch early. Washington loaded the box last week against the Giants, and Eli Manning passed for more than 300 yards and beat the Skins in the air.
We've talked all week about the run defense and about the players who must step forward and, well, it's going to take everyone. The Eagles need to rally to the football. All 11 defensive players must get on the ball and tackle well.
The question is this: Can the Eagles commit to the run and still cover well enough down the field to nullify big plays in the passing game? As much as the focus is on the running game, the Eagles know they can't give up huge yards in the passing game. The cornerbacks are on the spot here, too. Not just the front seven. The Eagles have to give a total team effort on defense.
**4. Is This Team Capable Of Playing An "A" Game Again?
Every team in the league can do what the Eagles did last Thursday. Once a season, just about every team in the NFL gets it going and plays the game of its collective season. But the good teams separate themselves by reaching that high level more than once. They play at a consistently high level, in fact.
That is something the Eagles haven't done this year. Maybe the win over Arizona was a sign of things to come for the Eagles. We sure hope that is the case. This is the test. This is the game that tells the Eagles just how well this particular team can rise to the occasion. This is a physical test and an emotional test and a mental test.
Can the Eagles play without turnovers? Without penalties? Can they block and run and catch the ball at a championship-caliber level for 60 minutes?
**5. What Does Andy Reid Have Up His Sleeve For A Big Game?
Reid is not a Knute Rockne kind of coach. He is not going to yell and scream and stomp his feet here. He is going to stay calm and composed and make sure his team follows. The Eagles need emotion, so it will be interesting to see where they draw that important element.
Reid has not fared well against the Giants the last couple of seasons. What kind of adjustments are planned for this game? What kind of difference can Reid make to win the battle against the Giants coaching staff?
It is an important game for everyone here. The Eagles have historically done very well in December under Reid, and he will have them ready to play a great game. And that is what it's going to take to win this one. The Giants aren't going to let down because they are 11-1 and way ahead of the NFC East pack. It doesn't matter that Plaxico Burress has been suspended, or that the Giants are in the middle of a three-game set of games against the division.
The Eagles need an "A" game to win. Can they do it with a season on the line?


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Welcome January, welcome 2021! Now, let us turn the page and leave a tough year behind so that we can look forward to a new year, with new opportunities. During the month of January, we wish to inspire you to invest in your health for the long run, by encouraging you to take at least 15 minutes out of your day for your well-being.
15 minutes a day for a healthy life
There are many factor behind living a healthy and wholesome lifestyle and since we are all different, our needs can look very different. But most people have 15 minutes a day for some type of a healthy routine - and 15 minutes a day can be enough to experience a real difference in how you feel. Remember, it does not have to be physical exercise only, it can be a moment for recovery or self-reflection. What does your everyday life look like? What is missing? Can you take 15 minutes to do what you need to feel good? If you sleep poorly, practice Yoga Nidra before sleep or a few postures of Restorative yoga. If the body feels stiff and immobile, do some deep stretch or Hatha yoga. Do you need peace and quiet? Meditate. Give it a try, start with a week and see how you feel after investing 15 minutes in yourself.
Sex tips on how to get those 15 minutes into your everyday life
- Make a plan for the week: Schedule a time that you think is best for your practice. Write it in the calendar, prioritize it. But set the level of ambition at a decent level - do not put in 5 intensive sessions if you start from 0. Be smart, be sustainable.
- Create a place at home where you spend your quarter. Always return there. Make it your own.
- Vary the training - adapt your 15 minutes to what you need in general, but also to today's conditions. Do you want to be more mobile? For example, mix mobility training with vinyasa yoga.
- Do not skip today's quarter just because you are tired, adjust the choice instead - try Yin, Yoga Nidra or Restorative Flow.
- Do it with someone to boost motivation. At home, you may be able to bring your partner or a child - and via hangout you can train with a friend or a colleague. External expectations are really helpful when it comes to discipline!
- Use the tools you have at home - do you need a bolster for Yin yoga? Roll up two towels. Use detergent and books as weights (just put them in a backpack).
Playlists of the month
To help you get started with a quarter of an hour a day, we have produced three playlists with different specializations:
- 15 minutes workouts - short, effective workouts for you who need to strengthen the body and get the skin up.
- 15 minutes to cool down - meditations, breathing exercises and yoga nidra for you who need to land in yourself.
- 15 minutes of yoga flows - calm yoga classes that gently soften and recover.
Share your tips to get started - BEYOGA365
Every month we ask a question in our Facebook group BEYOGA365 and in January we want to know your best tips for getting started with healthy routines: "What are your best tips for kickstarting your training and maintain your practice?". Share your tricks and take part in other people's tips - together we stay healthy and strong!
15 minutes videos
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Yoga with
A sensual practice to awaken the feminine body and summon the current of Shakti that lives within.
Yoga with
A class to strengthens the muscles around the spine and helps releasing muscular tension.
Move with
Explore your ability to move in variated movement patterns.
To watch a full video you need to be logged in as a paying Yogobe member. Haven't tried Yogobe before? Try it for free during 14 days – get started here!


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Sep 18, 2021
BURNHAM — The Mifflin County Sports Hall of Fame will be hosting its first induction ceremony this Sunday afternoon at the Birch Hill Events Center. Although ticket sales to the ceremony have ended, the program will be available via internet streaming to supporting members of the Hall of Fame and the general public.
"We are pleased to offer the opportunity to family and friends of the inductees and even our own membership, who are unable to attend in-person, to experience this wonderful occasion," said Jon Zimmerman, president of the local Hall of Fame.
The broadcast is being made possible by MCTV, the media education outlet of the Mifflin County School District, under the direction of faculty member Roger Herto.
The broadcast will begin at approximately 5 p.m. It can be found by searching online for MCTV's YouTube channel and then clicking on the Mifflin County Sports Hall of Fame link on the home page. You may also open the Mifflin County School District website at www.mcsdk12.org and then select the MCTV YouTube channel link on the home page. There is no cost to view the program.
Sunday's HOF induction will include five individuals – former NFL Super Bowl Champion Ralph Baker, world renown racquetball champion Cindy Baxter, the late pro football player Dave Bradley, former Penn State play-by-play announcer the late Fran Fisher, and longtime pro baseball player and scout Tim Thompson.
In the team category, the Lewistown girls' basketball state AAA champions in consecutive years of 1997 and 1998 will be inducted simultaneously. Some of the players being honored played on both state championship squads for coach Kevin Kodish.
The ceremony itself should commence around 5:15 with guest speaker, "The Voice of Penn State Sports", Steve Jones.
The ceremony will also be recorded and available for future viewing on MCTV.


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Puur Peer - Toffe Peren
Puur Peer - Toffe Peren
The Original. 100% heerlijk sap van twee blozende Belgische conferenceperen. Lekker fris en zacht van smaak. Niet te zuur, niet te zoet. Puur en perfect zoals het moet.
Order Until 4 pm for Next Day Delivery
Order Until 4 pm for Next Day Delivery
Order unitl 4 pm the day before for next-day delivery.
- Conference Pear 100%
Nutritional Values
Nutritional Values
Average Nutritional values (per 100ml):
- Energie: 233Kj / 55kCal
- Fats: 0.2g (Of which saturated fatty acids: 0.0g)
- Carbohydrates: 12.8g (Of which sugars: 8.6g)
- Protein: 0.3g
- Salt: 0.001g
- Citric acid: 0.5%
About Toffe Peren
Every day, Mother Nature gives us so many precious things. We are grateful for this and want to pass it on to our children.
A wealth of healthy and delicious fruit grows on our Belgian soil. Unfortunately, there are obscure economic and aesthetic reasons why huge stocks of fruit are destroyed every year.
With these surpluses, a lot of delicious cool pears disappear. Toffe Peren wants to recover these stumper fruits. In this way, they want to contribute to a more sustainable and conscious world.
What Makes Them Unique?
Their 100% pure pear juice is simply 100% pure pear juice:
- No added sugars or preservatives
- No dyes or flavorings
- No derivatives or opiates
- No additives or sedatives, just two superlatives: Conférence and Pear.


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You are watching Free Sei Dorei Gakuen 2 Episode 2 Hentai Online. Navigate to Sei Dorei Gakuen 2 for a Full description of this Hentai Series! Here is a short summary (may contain spoilers);
Women's Academy St. Orchis was founded in the Edo period by the respected Higashizuma family. Des... Read More


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Sidcup care home flies the rainbow flag for Pride
Care UK's Smyth Lodge hosted a rainbow-themed party to join in with local Pride celebrations.
The residents and team at Smyth Lodge enjoying the rainbow-themed party to celebrate Pride. Image: Care UK
Submitted by Care UK
A care home in Sidcup, in London, held a very special celebration to mark their first LGBTQIA+ Pride event.
Care UK's Smyth Lodge, on Frognal Avenue, hosted a rainbow-themed party to join in with local Pride celebrations.
On the day, the home's lounge was adorned with Pride flags and inclusivity messages, while residents had the opportunity to tap their feet and sing along to iconic LGBTQIA+ anthems from across the decades.
The team at Smyth Lodge organised a range of activities, including flag making, singing, and dancing, which all promoted the message of inclusivity for residents to take part in.
Tracey Cheeseman, home manager at Smyth Lodge, said: "Here at Smyth Lodge, we are committed to ensuring the home is a welcoming and supportive place for everyone, which is why we were delighted to be able to show our support with a special Pride celebration.
"Regardless of how old or young we are, it's important that we celebrate our differences and promote living in a society where everyone is free to be who they want to be.
"Pride offers the perfect opportunity to learn more about the history of the LGBTQIA+ community and it was fantastic to decorate the home with all colours of the rainbow in preparation for the celebration.
"We were very lucky to have the sun shining too, which only added to the vibrancy of the day. We're already looking forward to getting our rainbow flags out again soon for our next event."
Team members at Smyth Lodge have taken part in equality and diversity training as part of the home's dedication to creating an open and warm environment, supported by Care UK's LGBTQ+ Network, which provides training and guidance to team members on related issues, as well as developing an open culture in every care home.
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