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Stunning Beachfront Casa South Breeze 34
LocationRiviera Maya, Quintana Roo State: Private Home Puerto Morelos in Puerto Morelos
What You'll Love
- Private pool (with jetted tub) and beach.
- Quiet neighborhood.
- Concierge on site (Felix) lives close the property.
- Private beach access
- Perfect for honeymooners or big families
What To Be Aware Of
- No Smoking
- No Pets
- Private beach is narrow.
- crabs, haha yes we have in October.
- Master King Bedroom
- 2nd Master King Bedroom
- king Bedroom
- 4th Room Matrimonial Beds
Key Info
|# Full Baths
|Beach Front
|# Half Baths
|Property Type
|Sleeps (max occy)
|Sleeps in Beds (not counting temp beds)
|Getting to the beach
|steps away from our property just wake up and wet your feet in the beach.
|Hot Tub
|Other Amenities
|Wifi, Exceptional View, King Bed, Flat Panel HDTV, Gourmet Kitchen
|Antiquing, Beachcombing, Bird Watching, Boating, Bowling, Disco, Eco Tourism, Outlet Shopping, Paddle Boating, Photography, Scenic Drives, Shelling, Shopping, Shuffleboard, Water Sports, Wildlife Watching
|Partially Wheelchair Accessible, Children Welcome, Minimum Age Limit, Events/Weddings Allowed, Monthly Rentals Allowed, No Smoking, No Pets
|Sports Availabile
|Fishing, Freshwater Fishing, Golf, Jet Skiing, Kayaking, Roller Blading, Sailing, Scuba, Water Skiing, Snorkeling, Swimming
|Private Full Size
|Total Beds
|King Beds
|Queen Beds
|Twin Beds
Come enjoy this stunning beachfront hacienda equipped with all the modern amenities. This retreat, full of charm, incredible artwork, and traditional Mexican architecture is surrounded by a tropical paradise. Upon entering the front iron gate, you will cross a relaxing courtyard bursting with bougainvillea - a wonderful place to sit in the shade and read a book. From there you will enter through the two-story iron and glass front door into the beautifully tiled living space. The house is masterfully decorated with artwork. You will have your choice of relaxing in the living room or various other sitting spaces including two hammocks. The kitchen is fully equipped with modern appliances and beautifully tiled countertops. In addition, there is an additional room with a grill and sitting area which is screened in so you can enjoy the fresh sea breezes. The four bedrooms accommodating 8 let you enjoy natural light, full baths, and beautiful details including a screened in the sitting room connected to the master suite. Leaving the back door, you will find yourself looking at the crystal clear pool, and beyond. Just steps away you can enjoy your own private beach. Centrally located between Cancun and Playa del Carmen, this luxurious retreat will leave you rejuvenated and inspired.
This will be an experience to never forget, the SkyRun Mexico family offers you concierge services, shuttle to and from the Cancun International Airport, private chef, property host to walk you thru all the local activities ad offer you any information you may need to make the most out of your dream vacation.
We are here to answer any questions and to help you make this new adventure possible.
This incredible property has an amazing old style decor perfect to enjoy the real mexico close to quiet town like Puerto Morelos where you can seat and enjoy great breakfasts, coffee, sunsets, boat trips or just lay down in front of spectacular beaches; our awarded concierge staff and chefs could make this trip and unforgettable experience if you come to mexico for anniversary, birthdays or just holidays.
Fernando, Giovanna and Feliciano will help you to have a non stress vacation time with us, where you will forget everything and just enjoy our property.
our Skyrun team has been awarded by airbnb, trip advisor and others as best for years like super host, please let us show how magnificent could be to stay with us!!
Getting to the beach: steps away from our property just wake up and wet your feet in the beach.


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Wagh Bakri Premium Leaf Tea, this amazing tea is handpicked from the best tea gardens and blended by tea experts. Wagh Bakri Premium Leaf Tea ensures a strong taste, rich colour and a stimulating aroma.
Food Memories tries its best to provide precise product information. However, many times, the manufacturers tend to make changes in their ingredients list. Detailed information about the ingredients and materials can be found on the actual packaging of the products. It is recommended that you do not rely solely on the information provided on the website pertaining to the products. Before consumption and usage, please read the labels, warnings and directions printed on the product packaging.

subset: commoncrawl


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Main Index
Media Index
Jonny Greenwood
UK Media
2012 Interviews
BBC Radio 4 'Front Row', march 23rd 2012
[Interview with Jonny and Krzysztof Penderecki]
This is a transcript from an audio recording of the broadcast on march 23rd 2012, the interview was recorded march 22nd.
John Wilson
[Narrating]: The Polish composer Krzysztof Penderecki was a pioneer of 20th century experimental music, developing new tonal scales and melodic structures in the 1960s. His music is best known for its use by Stanley Kubrick in the film 'The Shining', it also provided a terrifying soundtrack to 'The Exorcist'. Now Penderecki is collaborating with one of his biggest fans – Jonny Greenwood, guitarist with the band Radiohead – who's also forging a career as a classical composer. The pair have released a record together, and last night staged a joint concert at the Barbican in London. I met Greenwood and Penderecki in what we hoped would be a quiet dressing room at the Barbican. I started by asking the 78 year old composer what he was trying to achieve musically in 1960s Poland.
I was young, angry, I wanted to build my own world, forget about polyphony and harmony for many years, and I was trying to write my music.
[Excerpt from a piece]
And did you say you were 'young and angry' or 'young and hungry'?
Maybe both but... [laughs] I was angry because, you know, this was a time of rebellion, a time of discovery... not only me but everybody at that time, young people, especially in Europe. So we wanted to start from the beginning.
A lot of people may think they are unfamiliar with Penderecki, and yet if they've seen films including 'The Shining' and 'The Exorcist', they will know these pieces of music very well. Very evocative and very scary. So did you welcome the use of your music in those films?
Yes, I remember... it was maybe 30 years ago... Kubrick called me and asked me if I would be interested to write the music for The Shining. And I gave him some proposal pieces that he should listen to, maybe it would be useful for him. And he did, it connected with the picture and sounds very scary.
So you don't mind people reinterpreting the music, taking it out of the original context then?
No. I mean, you know there are some people who know my music because of the movies.
Jonny Greenwood, when did you first come across the music of Krzysztof Penderecki?
It was just before we signed a record contract with EMI, and I did a few weeks at music college, and a lecturer played us 'Threnody' and showed us the score and said this is what orchestras can do as well. I took the score with me and just investigated while I was touring.
What appealed?
The complexity, the fact that you can make use of that many musicians in a room and do something that is so far removed from what I thought orchestras were. I didn't realise that you could make these magical sounds and textures.
[Narrating]: Jonny Greenwood is so enthralled to his music hero that he's composed 2 musical works in response to Penderecki. This one featured in the soundtrack to the film 'There Will Be Blood' starring Daniel Day Lewis. But he says that his sense of musical adventure started even before he joined Radiohead.
I remember being at primary school and having a violin orchestral lesson, and the teacher telling everybody "right, now everyone, can you try and make a different sound with your instrument?" And that fascinated me and I thought that's really exciting to think of what you're holding as a way of making other sounds. So, I've always thought of the guitar as a piece of technology, as something physical to get sounds out of, rather than this slightly fetishised obsession. It's like a tool, like a typewriter or something, but also quite limitless what you can do with it.
[Extract from Paranoid Android]
Before you met Jonny Greenwood, were you aware of Radiohead's music?
Yes, from my children and granddaughter actually, they told me and they gave me the music.
And what did you think of Radiohead?
It's very interesting, and you know this was for me... also discovering something new.
Are you increasingly drawn to the classical concert hall rather than the rock music studio, do you think?
What I've realised is that recordings just aren't good enough. I grew up being told that if you had the best recording of an orchestra, that you knew what it sounded like. But when you're sitting in a concert hall and you hear an orchestra begin, you realise that it's a far richer and more complicated sound than you can get from a couple speakers in a room. I mean, I nearly feel like i've been sold a lie. It's such a revelation to me, and that's why I would, you know, urge people to go to more classical concerts and hear orchestras live. I think Penderecki's music especially when you hear it on record and also how it's described is often that it's somehow abrasive, but it really isn't, it's just very rich and complicated and I think that's fascinating to see an orchestra play.
Can we hear any direct Penderecki influence on individual Radiohead songs, do you think?
I suppose so, I mean there's string arrangements in a song like 'How to Dissapear', which is on our album Kid A, and that ends with lots of microtonal effects and things that are very indebted to this kind of writing.
[Extract from HTDC]
I mean, even as early as Climbing Up The Walls on the album OK Computer, the strings on there, again, are very influenced by that complexity you can get.
[Sound of pneumatic drill in the street outside]
Some strange noises off in the Barbican. [Laughter]
That's the first chord.
You like that sound, you could use that.
Oh, yes.
[Narrating]: Well, the sound of a pneumatic drill on the street outside the Barbican may provide inspiration for an avant-garde composer, but it's not good for a Front Row interview. So we moved from dressing room to the concert hall itself.
...And you broke the rules when you first started writing this kind of music, was it an act of rebellion against the Communist regieme of the day?
Yeah, we were trying really to... this was not only happening in the music at the time, it was in the theatre – very modern, avant-garde theatre – it was the movies, everything, and also music. We were trying not to follow the kind of aesthetic which they asked us to do.
And are you still trying to break the rules now, in your later years is it still important for you to be...[Penderecki's mobile rings]
That's a very conventional ringtone, isn't it.
There isn't a Threnody one yet.
There's no Polymorphia ringtone... that's a shame, isn't it? [Laughter] Is it still important for you to be exploring new ways of making music?
Yes, but of course not the way I did 50 years ago. I'm trying to, first, not repeat myself.
And Jonny Greenwood, what is the game plan for Radiohead? I know you're mid-way through a tour. You'll be heading back to the studio to make another album soon?
No, we're touring 'til November, and we've got no plans after that, so...
And do you have a future vision of the direction the music will head?
I dunno, it's confusing because half the time I'm very excited to be programming and using computers, and I love that side of what we do with Radiohead, but then I keep coming to classical concerts and getting very excited about acoustic sounds. I feel the two things splitting apart, which is interesting to me.
Could you see a Radiohead/Penderecki collaboration sometime in the future?
I doubt it. He writes every day, the professor, and has many commissions ahead of him.
No, actually we have some future plans. I think in the next year – I don't know if you are going to do it – but I would like to write a piece for string quartet, electric guitar for you, harp and double bass, so a septet.
So a real collaborative piece. Did you know about this Jonny?
Yeah, it's going back to the style of the first string quartets, which were very interesting to me, and if anything it's going to make me look at the electric guitar in a different way, it's going to be working with the professor on this kind of music... you know, it continues to inspire me.
[Extract from HTDC]


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WILLCOX Cowboy Hall of Fame inductees
Ralph C. Cowan was born in Tombstone on April 3,1899, the son of William and Florence Cowan, a pioneer Cochise County ranching family. Ralph represents the second generation in the Cowan family, which has been involved in over one hundred years of ranching in southern Arizona. Ralph's father, William Cowan, was born at Covey Hill in the province of Quebec, Canada in 1857. After the American Civil War, the "go west" fever struck him. In 1879 he went to Virginia City, Nevada and later moved further south to Tombstone. He started the NI outfit in 1884 with two milk cows he first brought to Tombstone with the NI brand. A miner and banker as well as rancher, Ralph's father was well known for his thrift and his capacity for hard work. He instilled these qualities in his son. Ralph has instilled the same qualities in his three sons William R. "Bill, James H. "Jim" and Robert E. "Bob".
William Cowan had the foresight to understand that the federal government would create and administer regulations for livestock grazing on the federal domain. With that in mind he began buying neighboring ranches and homesteads comprised of deeded land. In doing so, he left a tremendous legacy to his son. Ralph added further to the original holdings.
Ralph spent his school years at Tombstone, Arizona. As a youngster, he rode as "rep" man for the NI's when the neighbors gathered. He was in charge of the chuckwagon at the age of 19.
Ralph married Mattie Geers, a native of Paolo, Kansas who had moved to Gleeson in 1918 to visit her brother. Ralph and Mattie were married on Christmas Day 1921, a union that lasted for more than 56 years.
Mattie soon found out that she had not only taken a husband for better or worse, but she had wedded the whole cattle business. To the Cowans, cattle ranching was their life. In 1939 Mattie founded the Cowbelles, which became a National organization.
The first few years of Ralph and Mattie's marriage were spent living from cow camp to cow camp as drought forced cattle to be moved from Arizona to Sonora, Mexico, to Texas, back to Sonora and finally back home to Arizona in 1925. In 1926, when Bill was two-and-a-half, the family settled in the house at the NI headquarters near McNeal. The Sulphur Springs Valley received an influx of homesteading settlers who hoped to dry farm, and inevitably failed in the brittle climate. Ralph and Mattie bought out a number of homesteads and he also bought the old Reeves Ranch and the JO near Tombstone in 1932. The Cowans raised many of their own registered Hereford bulls and registered Quarter Horses, at times requiring as many as 150 head to keep their cowboys mounted.
Ralph was appointed to the Arizona House of Representatives in 1939 to fill a seat vacated by a death and served through 1940. He then ran for the Senate where he served for some 20 years. Ralph was a tireless advocate for the cattle industry and spent many hours at the Adams Hotel where much of the state's business was conducted. Ralph served as president of the Arizona Cattle Growers Association in 1952 and 1953. He was extremely active in the American National Cattleman's Association. He was also a Mason, Shriner and a Past Grand Patron of the Eastern Star. Ralph passed away at home in December 1977.
Ralph's youngest son, Bob is still in the cow business, living at the JO in Tombstone. Bill and Jim live in Tucson. Ralph had seven granddaughters and four great grandsons and two great granddaughters.


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5th Annual Fairhope Film Festival
Fairhope Film Festival at Downtown Fairhope
The 2017 Fairhope Film Festival is from November 9 – 12, four days of brilliant, entertaining and award-winning films screened throughout our beautiful little town on the Bay! The focus of the Fairhope Film Festival is on national and international film festival competition finalists of the past year: the "best of the best" in cinema arts. Notable foreign and feature films, documentaries and shorts-many that never made it to the big box theaters or were only there briefly–will be selected for appreciative audiences. Although the festival will pull out all the stops, Southern-style, to host opening and closing events and parties, the emphasis will be on the art of film-making and the experience of seeing exceptional films. Directors, actors and screenwriters will participate in the screenings both in person and via live electronic transmission.
-> More Info…


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Really, Fox?
In the age of Google and internet searches, when one can confirm whatever piece of information they want in a matter of seconds, Fox Sports appears to have found a way to confuse one entire city with another during its coverage of the FIFA Women's World Cup.
A screengrab, which is said to have come from a Fox Sports broadcast, shows a built-up Vancouver skyline with a "Moncton, New Brunswick" banner plastered just below.
Besides the fact that the two cities are indeed sites for World Cup matches, they literally have nothing in common. Here's what Moncton actually looks like:
The image was quickly posted to Reddit, where it got the mocking it so deserved.
"Moncton, NB: Year 2150," one user wrote.
"I was thinking 2500 lol," replied another.
To make matters even more embarrassing for the U.S. network, Fox Sports actually built a two-storey broadcasting studio in Vancouver to be its headquarters for World Cup coverage. You'd think someone would have noticed the striking similarity between Moncton and their view from outside the window.
Ah well, guess that's why we stick with Canadian media.


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Please mark your calendars for Saturday
July 20, 2019 for the Sandcastle Run.
Bob Hall Pier
Race will Start Promptly at 8:00 AM
PET Friendly!
LATE/DAY OF Registrants: Please bring cash or check (made out to 'American Diabetes Association') as we will not have credit card readers! Thank you for supporting this worthy cause!
Brent Chesney's Sandcastle Run is a 5k run and walk to raise awareness for children struggling with Diabetes. All proceeds are donated to the American Diabetes Association's Camp Sandcastle. Camp Sandcastle gives children with diabetes the opportunity to enjoy outdoor activities and a wonderful summer camp adventure. Every dollar raised from this race stays right here in the Coastal Bend.
Friday July 19, 2019
Mikel May's Beachside Bar and Grill
15820 Park Road 22
Corpus Christi, TX 78418
5:30pm to 7:30pm


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Learn English in Toronto and Contact Us Today!
Get in touch with CES Today with any questions or learn the next steps to becoming a student. We will get back to you promptly!
School & Mailing Address
Capital English Solutions (CES)
543 Yonge St – Second Floor
Toronto, Ontario
Canada, M4Y 1Y5
For general inquiries, please contact
(416) 483-3738


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A section of the Rashee Road in Ballyclare is set to be closed from next week to allow for works.
Improvement work is scheduled to commence on the road from Readers Park entrance to Charles Drive on Monday, March 25 for approximately six weeks.
The temporary closure will run continuously from 8am on Monday, March 25 to 6pm on Friday, May 3.
The purpose of the closure is to facilitate water main replacement works.
Work will then be suspended during the busy exam period to reduce disruption for local schools and will recommence on June 24 until mid-August to complete the remaining watermains improvement work between Hillmount Avenue and Ballyeaston Road junctions.
In order to complete this work efficiently and safely, traffic management will be in place. Works will be completed under a lane closure with traffic lights, operating a stop-go system during peak flow. The work will be completed in sections to keep disruption to a minimum.
Pedestrian access will remain in place at all times. Unfortunately, on-street parking will be suspended within the works area with NI Water saying they will work with the local community to minimise disruption.
A Northern Ireland Water spokesperson said: "NI Water and our contractor, Farrans will hold an information event in Ballyclare Town Hall on Thursday, April 4, 5pm to 7pm, to discuss the works and upcoming schemes including Ballyeaston Road, Victoria Road, Hillmount Avenue, Erskine Park, Margaret Avenue, Charles Drive and Rathmena. Our team will be happy to answer any questions the public may have.
"NI Water would like to thank the public for their patience and cooperation during this essential improvement work and assure the public that we will do everything possible to keep all disruption to a minimum."


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4 - 4
The 6 Series is the perfect fusion between technology and design: engineered with built-in efficiency in every single system and with minute attention to detail in every component, instrument and control, these machines are extraordinarily productive. The range also includes agile, more compact models with a shorter wheelbase that are ideal for any application, to match this superlative efficiency with remarkable versatility. With powerful Deutz engines, tried and tested Powershift transmissions and instantly recognisable, attractive style by Giugiaro Design with clean, bold lines, the 6 Series sets the standards for others to follow.
With a highly modular configuration to cover a wide power spectrum, these engines feature state of the art solutions for maximised performance: four-valve cylinder heads, turbo-intercooler forced induction with wastegate, DCR (Deutz Common Rail) high pressure injection system (up to 1600 bar) with integrated electronic control and SCR catalytic converter exhaust after-treatment system. Combined with the special combustion chamber configuration, DCR maximises performance and ensures constant power delivery even in the most demanding conditions, with superlative torque on tap even at very low engine speeds. Deutz engines are engineered and built specifically for agricultural use, with maximum torque already available at 1600 rpm and maximum power produced at 1900 rpm, while close to maximum power is available almost continuously from 1600 to 2100 rpm - effectively the entire operating range used in the majority of field applications. The SCR catalytic converter maximises efficiency by eliminating the need for exhaust gas recirculation, reducing fuel consumption by up to 10% compared with previous versions of equivalent power outputs. This considerable saving in fuel consumption more than compensates for the cost of AdBlue additive (a 32.5% solution of extremely pure urea in demineralised water) needed for the SCR system to function, minimising both running costs and environmental impact. And for even greater environmental sustainability, all 6 Series models are fully compatible with fuels containing up to 100% biodiesel.
The Powershift transmission offers four speeds in each range. The APS (Automatic Powershift ) feature automatically selects the ideal powershift speed in relation to load and engine rpm. The rapid response changes of the powershift are effective during operations which require frequent shifting. This lets the driver concentrate fully on the job in hand while the system optimises engine efficiency to reduce fuel consumption. Automatic 4WD and differential lock control gets the machine out of even the trickiest situation.The system automatically engages or disengages 4WD and the differential locks in relation to speed and steering angle. This not only maximises traction for jobs in the field, but also ensures good manoeuvrability.
The S-Class² cab of the new 6 Series is the next evolution of the legendary Agrotron cab. Generous sized windows and screens combined with narrow posts offer perfect 360° visibility. The all-new interior boasts attractive colours and superior quality materials. Numerous storage areas are located logically around the cab and allows documents to be stored safely, while the drinks cooler is big enough even for large bottles. Large screens and windows, rounded mudguards and a sloped hood form the principles of the DEUTZ-FAHR clear view concept, which allows the 6 Series to offer a high degree of unobstructed all-round visibility that is normally the reserved for higher power categories.
Other features include:
The hydraulic system uses 120l/m load sensing pump to feed the distributors and lift, and a 42 l/min pump dedicated to the hydrostatic steering system, for smooth and effortless steering even when other hydraulic utilities are operating simultaneously, and in critical operating conditions with the engine at idle speed.
The base configuration of the system includes a pair of mechanically controlled dual action distributors (or, as an option, four), and may be extended with the addition of a third electronically controlled distributor and two more front distributors. "P" versions come with four, dual action electronically controlled distributors (or, as an option, up to seven).
All the distributors may be locked if needed and switched between single and dual action mode, even with the lift in use.
For more information:
Mark Bond (Sales) E: firstname.lastname@example.com Ph: 03 5352 1643
Terry Peatt M: 0417 300 548 Ph: 03 5352 1643 Kerry Hammond Ph: 03 5352 1643
Ararat Auto & Ag Services and Ararat Automotive Services - 133 HIGH STREET ARARAT VIC 3377
PH: 03 53521643 | EMAIL: email@example.com |
© Ararat Auto & Ag Services, 2018


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CTC is a cooperative with offices in Brainerd and Baxter. Their goal at CTC is to offer the latest technology and highest quality of service for your business and home phone, high speed internet, and digital TV services.
CTC will be the premier provider of communication solutions providing products and services that enhance our customer's personal and professional lives.
CTC is committed to providing advanced communication solutions, with the highest quality of service, for our customers. CTC's employees inspire one another to support and strengthen the communities we serve.
Integrity – Responsibility – Cooperation – Respect – Service
CTC has over 55 years of telecommunication experince right here in the Brainerd Lakes Area. With CTC implementing the areas first fiber optic network, CTC supports their customers from start to finish on all installations and repairs. Their network allows them to offer the highest dedicated internet speeds and Centrex Cisco IP telephone systems.
High Speed Internet
Digital TV
Local and Long Distance Service
Centrex Phone Systems and Service
Computer Networking
At CTC we're Connecting The Community!
Technology Center: North of Cub Foods on Highway 371. Corporate Office: Brainerd Industrial Park on Madison St.


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If the beaches at the North Sea are too far for you, check out these city beaches in Amsterdam. Urban beaches are perfect for a quick get away from the crowded city life to enjoy the Amsterdam sun at its best: in your bikini. Dip your feet in cool waters while sipping a cocktail and watching the kids build sand castles.
RECOMMENDED READ: Beaches near Amsterdam
Strand Zuid City Beach
Strand Zuid is located at the south side of Amsterdam, near the business district of the Zuidas and next next to Rai Convention Centre. It attracts a successful business crowd. Expect quality sun beds, hammocks, beach chairs and games.
On the sand beach you can play volleyball, table tennis, jeu de boules and the Dutch game sjoelen. The restaurant serves breakfast, lunch and dinner.
The city beach of Strand Zuid is a spacious area bordering a pond and the lovely Beatrix Park. In summer, the Strand Zuid organises special events like movie nights and themed parties. Check the website for the agenda.
How to get to Strand Zuid
Strand Zuid can be accessed by from the center of the city with metro 52 (Europaplein), metro 50, various buses or by train at railway station RAI.
City Beach Pllek at NDSM
At NDSM, a former ship wharf across the IJ waters, is the hugely popular Pllek. The large restaurant and bar is located along the water and has terrific views. Pllek is busy all year round, offering its clientele live music every Sunday afternoon, yoga classes on Sunday morning, club nights on weekends, art exhibitions and more.
In summer the program of Pllek expands outdoor. On the large sand beach there are many comfy bean bags and opportunities to worship the sun. There are festivals and a weekly open air cinema (every Tuesday). When the water warms up you can jump right in!
How to get to Pllek
Amsterdam City Beach Pllek is found at NDSM. The free ferry takes you there in 15-minute and leaves from behind Central Station.
RECOMMENDED READ: Outdoor swimming in Amsterdam
Rooftop beach at NEMO
Not really a beach, but still, the playground created by NEMO science museum on top of its roof has all the ingredients to enjoy a day in the sun: lounge chairs, ice-creams, olive trees and, of course, cocktails. The roof top has beautiful view over the city center.
Especially children will not get bored at this urban beach because of the highly entertaining watery playground Energetica. Kids can splash around with water in an educational and scientifically justified manner. Fun with molecules!
How to get to city beach NEMO
City Beach Nemo is located on top of the green ship-like building the NEMO Science Museum. It's at Oosterdok, a 10 minutes walk from Central Station. Open daily until September from 10.00 to 18.30. Oosterdok 2 (free entrance).


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The following article popped up late last week on ZDNet. It is a very interesting summary of a 30 minute or so podcast.
Free broadband will help the NBN's case
By Phil Dobbie, ZDNet.com.au on October 28th, 2010
Some of the major benefits to society from a ubiquitous broadband network will come from government services, including health and education. That means access to these services needs to be free, so everyone is able to make use of the channel, thus displacing other, more costly, ways of interacting.
This isn't a new notion. Economist Joshua Gans raised it over a year ago at a Senate Select Committee hearing in Melbourne. He suggested the government provides free access to a basic internet service that included public services.
Industry analyst Paul Budde agrees that we should be working towards a free government network, available to all households through the National Broadband Network. For example, e-health services could be available to all, without needing to connect to a commercial retail service provider.
Simon Hackett, managing director of Internode, is less convinced about the idea of a direct connection for government services. He says we only have one network in the home, which means only one connection to the NBN.
Paul Brooks from Layer 10 Advisory disagrees, arguing that we have multiple networks into the home — television, our phone, internet. A government-provided network could be another one.
This week I ask whether we need free government services for everyone to realise meaningful benefits from the NBN and, if so, why is no one doing anything about it?
More here:
You can play the podcast (narrated by Phil Dobbie of ZDNet's Twisted Wire and his guests) here:
The key issue from the e-Health perspective is that to date there has been very little recognition of the whole 'digital divide' issue - where the old and the poor frequently lack any internet access and so cannot utilise things such as the proposed PCEHR etc.
The discussion provides some useful insights to the possibilities that may be explored.
It also points out that for some reason these ideas are not really being discussed much in the halls of power. That indeed is a pity if true.


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The Tiny Clear Blobs Washing up on Beaches Are Even Weirder Than You Think
Contrary to popular belief, these harmless creatures have no relation to jellyfish.
Thousands of small, gelatinous, crystal-clear blobs are washing up on East Coast beaches. Though they're often referred to as "jellyfish eggs" these weird little creatures are called salps, and they have more in common with people than they do with jellyfish.
"As you enjoy the warm water today, you may come across some of these jellyfish-like critters. They are called salps, and are present because of phytoplankton blooms, which are their food source," Kill Devil Hills Ocean Rescue wrote on Facebook alongside a photo (below) of a hand holding a number of salps. "The black dot in the center of them is their digestive system, and they are completely harmless."
Changes in wind direction or water currents will push them onto shore, but as far as blob-like ocean creatures go, they're an absolute breeze.
Though they resemble jellyfish, "the only thing salps and jellyfish have in common is that both are gelatinous and both float around in the ocean," Larry Madin, executive vice president and director of research at the Woods Hole Oceanographic Institution, told National Geographic.
Salps, like their relatives the sea pork, are part of a group called tunicates, are considered one of the most evolved of all marine invertebrates. They have a kind of primitive backbone, which jellies lack. They're just there to chow down on algae.
So, fear not the slimy salp!


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April 19, 2016
Steve Droy
steve Droy
Polyester Play Day
November 26, 2018
How to tune a piano by ear
October 15, 2015
On the road in New South Wales; the journey so far..
February 1, 2019
We are growing!
May 7, 2019
A visit from I.M.I.T
October 24, 2018
Its beginning to look a lot like..2018 course will be FULL!
December 11, 2017
Zen and The Art of Piano Technology
November 22, 2017
Re-Stringing an upright
April 25, 2017
Andy's career as a Piano Tuner gets kick start!
November 21, 2016
A Journey into Sound
October 11, 2016
Good luck class of 2016!
September 21, 2016
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NV Connie Mack reaches state semi
Thursday, August 10, 2017 by CHRISTOPHER HIPPENSTEEL in Sports
Northern Valley's bid in the Connie Mack state playoffs did not get off to an auspicious start. In their opening matchup, the Chargers fell to a lower-seeded Nazareth team by a score of 11-2, placing them on the brink of elimination. With one loss already on its record, Northern Valley would have to remain undefeated if they were to keep its championship hopes alive.
Ultimately, the Chargers wouldn't take home this summer's state title, but they came closer than anyone could have predicted after the opening loss.
NV Connie Mack seeks playoff run
Wednesday, July 12, 2017 by CHRISTOPHER HIPPENSTEEL in Sports
When Northern Valley stepped onto the field for its matchup against Northampton, the Chargers knew that the next seven innings carried heavy implications for the upcoming Lehigh Valley Connie Mack postseason.
The Chargers entered the contest with momentum on their side; with three consecutive wins under their belts, the players felt confident they could continue their streak and climb a bit higher in the standings.
Despite their positive outlook, the Chargers would ultimately fall to Northampton 5-3 in a well-fought defensive showing by both teams.


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Fans, get your favorite Disney/Pixar Cars locations, characters and tracks in one exciting pack! This ultimate Radiator Springs story set delivers hours of racing and stunt fun! Open it up and cruise through Willie's Butte track, then boost your speed at the Racin' Route 66 launcher. Rest up at the Piston Cup Pit stop or rev up at Flo's V-8. When you're ready for action, brave the loops, ramps and more at Sarge's Stuntin' Surplus or dare Grey's Transporter stunt set.
Amazon Prime Eligible
Don't have Amazon Prime? Try it for FREE! Try Amazon Prime 30-Day Free Trial. Comes with Free 2-Day Shipping!


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Since it was established in April of 2000, AWMA Pty Ltd has evolved into Australia's leading manufacturer of aluminium and stainless steel water control infrastructure. Offering complete water control solutions across a variety of sectors including dams, desalination plants, mining water treatment, environmental and many others, AWMA continues to provide quality solutions and services to the nation's water industry.
Steve Laracy founded Larpro Projects in May 2006 with "a desire to do better." He had worked for several major contracting companies in the past and "had always thought of doing things a bit different." By early 2007, the fledging project management company already had a number of projects under its belt and was growing rapidly.
Australian Fibreoptic Communications
Since the 1970's, fibre optic communication has revolutionised the way people communicate and the way companies do business. The move from traditional copper wiring to fibre optic cable has allowed greater amounts of information to flow across Australia and has brought millions of people within virtual reach of one another.
Modular Building Systems
Many companies are currently in the business of ordering accommodation for installation in remote areas; but, in the view of Glenn Smith, Chief Executive Officer of Modular Building Systems, many are underestimating their options.


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Learn More
Thermo Scientific RadEye B20 and B20-ER Multi-Purpose Survey Meters
Quickly characterize mixed surface contamination from gamma radiation, x-ray radiation, alpha radiation and beta radiation with dose rate measurement survey meters.
Manufacturer: Thermo Scientific 425068510
First responders need to quickly identify mixed radioactive surface contamination in facility and field environments. Thermo Scientific RadEye B20 and B20-ER Multi-Purpose Survey Meters are simple, robust, reliable contamination and dose rate measurement tools for characterizing alpha, beta, gamma and X-ray radiation. RadEye B20 and B20-ER Multi-Purpose handheld Geiger counters provide an on-site solution for homeland security personnel, fire brigades, emergency response personnel, agencies involved in decontamination and decommissioning projects and hospital and pharmaceutical industry employees.
RadEye B20 and B20-ER Multi-Purpose Survey Meters offer consistent, reliable results in any application requiring radiological surveys of flat surfaces. Model B20 is for normal measurements. Model B20-ER is for high-range measurements.
- Lightweight (300g), excellent grip with and without gloves.
- Rugged and compact design, thick rubber protective cover.
- Low cost of ownership with > 500 hour operation time with 2 AAA batteries rechargeable NiMH-cells can be used.
- Menu-driven user interface results in low training cost and immediate familiarity.
- Huge internal data memory for both scaler results and continuous data recording.
- Bright backlit LCD display. Plain text messages and different languages can be selected.
- Easy adaptation to different tasks by supervisor configuration, calibration, selection of measuring units.
- Versatile operation modes:
Continuous ratemeter mode for frisker operation.
Dose rate mode.
- Audible indication: single pulse or chirper mode proportional to count rate.
- Earphone output for operation in loud environment.
- IR PC Interface or Bluetooth as option.
- Advanced Windows software is available as option.
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|5.2 x 2.8 x 5.2 in. (13 x 7 x 13cm)
|Survey Meter
|RadEye B20-ER Multi-Purpose Survey Meter
|2p Efficiency (ref. to 50mm diameter without rubber sleeve): Am-241; Co-60: 25%; Sr/Y-90: 36%; C-14: 19%
|0.7 lb.
|2.8 in.
|Contamination B-20: 0 - 10kcps; B20ER: 0 - 500kcps
|More than 500 hours on two AAA batteries
|LED, sound, vibrator
|1.7 in.
|Gamma Dose Rate: 0 to 100mSv/h (0 to 10 rem/h); Contamination: 0 to 500kcps
|Gamma dose rate: 0-100mSv/h (0-10rem/h); Contamination measuring range: 0-500kcps
|13 cm
|Civil defense, fire brigades, hospitals, nuclear industry, and pharmaceutical industry
|Pancake GM-tube, window dia. 44 mm (1.7 in.) 1.8 to 2.0mg/cm2
|5.2 in.
|2.4 in.
|17 keV - 3 MeV; according to IEC 60846-1)
|LED, sound, vibrator
|2 AAA batteries
|100mSv/h (10rem/h)
|5.2 in.
|cps, cpm. Bq, dpm, dps, Sv/h, rem/h
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Crystal growing apparatus
An apparatus for growing diffraction quality, protein crystals by vapor diffusion techniques. The apparatus includes a plurality of equilibrating solution reservoirs each having a side wall terminating in a top wall disposed in a first plane; a column disposed within each of the equilibrating solution reservoirs, each column having a protein solution receptacle including a side wall terminating in a top wall disposed in a second plane spaced from the first plane; and a cover having a non-greasy adhesive on one surface therof for sealable engagement with the top walls of the equilibrating solution reservoirs for sealing the reservoirs relative to atmosphere.
Field of the Invention
The present invention relates generally to an apparatus for growing crystals. More particularly, the invention concerns an apparatus for growing diffraction quality, protein crystals by vapor diffusion techniques.
Discussion of the Invention
Crystallography is a powerful analytical tool which provides precise and detailed descriptions of the structure of molecules. Over the last thirty years, small molecule crystallography has been successfully integrated into chemical research at both the basic and industrial levels. Crystallography of macromolecules is now increasingly being viewed as the ultimate analytic tool of life scientists and is expected to play a very significant role in the biotechnology revolution.
In the past, several major technical difficulties have prohibited the routine use of macromolecular cyrstallography and its complete integration into the mainstream of biological sciences. These include problems associated with data collection from X-ray diffraction instrumentation, the computational analysis of the data, and the actual growing of the crystals. The successful introduction of are detectors for the extremely rapid collection of data has significantly reduced the data collection problems. Similarly, the enormous increase in computing power and the development of sophisticated computer graphic displays have substantially reduced the problems associated with computational analysis of the data. The remaining problem, crystallization, was initially circumvented by analyzing only those macromolecules which could be obtained in abundance and which crystallized readily. The structures of macromolecules possessing those qualities have long since been solved and today, the crystallization step remains the last major barrier to the routine structural analysis of commercially significant macromolecules.
One of the most common prior art vapor diffusion methods for growing crystals is the so-called "sitting drop" method and uses a glass plate with a plurality of depressions that act as wells for the protein drops. The plates are expensive and therefore are typically washed and siliconized for every trial. A clear plastic sandwich box is typically used for the equilibration chamber. The glass plate is placed on an inverted petri dish to elevate it above the solvent in the chamber and is then sealed with grease. The carrier contains protein drops equal in number to the depressions in the plate and each drop is equilibrated against the same solvent reservoir.
Another prior art method, sometimes called the "hanging drop" method, uses a multi-well plastic cell culture plate. This method allows a single protein drop to equilibrate against its own unique solvent, as opposed to all protein samples equilibrating against the same solvent, as is the case in the "sandwich box" method. In the "hanging drop" method, each drop is placed on a glass cover slip, which is carefully inverted and placed over a greased well to seal it. This "hanging drop" method is generally superior to the "sitting drop" method, but is quite awkward and significant problems are presented in removing the crystal from the greasy cover slip.
Still another prior art vapor diffusion method for growing crystals is the so-called "sandwich drop" method. In accordance with this method, the equilibrating solution is contained within a suitable reservoir and the protein drop is sandwiched between two glass plates which are sealed in position within the apparatus using vacuum diffusion pump oil. The technique of forming the sandwiched drop with parallel flat surfaces perpendicular to the light path provides improved microscopic viewing. However, this method is costly, somewhat tedious and the necessity of using a sealing medium such as pump oil provides an undesirable source of possible contamination. Further, the requirement for sealing two glass plates substantially increases the risk of vapor leaks causing the protein drops to dry out.
The aforementioned prior art, manual methods clearly illustrate the fact that, to date, the crystallization of proteins has been largely an empirical practice. The probability of success of the particular prior art method is often proportional to the number of trial samples composed. Frequently, the total number of variables such as concentrations of protein, salt, and inhibitors along with physical parameters, such as temperature, require many thousands of attempts in order to properly sample all the possibilities and combinations. Success, if it is achieved at all, sometimes demands a nearly intolerable amount of technical application and hundreds of hours of tedious human effort.
To obviate the labor intensive requirements of the prior art methods, attention in the field has turned to automation of the crystallization trial procedure. Systems for this purpose, for both dispensing the trial solutions and their later analysis for the presence of crystals, have been designed using a variety of robotic and optical scanning procedures However, for various reasons, the prior art methods do not easily lend themselves to automation. For example, robots have substantial difficulty in manipulating small glass components and properly sealing containers with sealing mediums such as oil and grease. Further, the prior art apparatus is generally not compatible with automatic pipetting equipment, or with automated optical systems used for data collection. Additionally, because of the basic design of the prior art apparatus, the kinetics of the equilibrium process are often difficult to control and reproduce.
The apparatus of the present invention overcomes many of the drawbacks of the prior art methods and devices by providing novel crystal growing apparatus which is inexpensive, easy to use and readily compatible with automatic pipetting, sample preparation and diffraction analysis equipment. Because of the unique design of the apparatus, laboratory technicians with no particular skills, or specialized training, can use the apparatus to grow high quality crystals in very large numbers for numerous uses, including the diffraction analysis of macromolecular structures.SUMMARY OF THE INVENTION
It is an object of the present invention to provide an apparatus for growing protein crystals by vapor diffusion in which a multiplicity of crystals of precise size can simultaneously be grown with each crystal being grown within its own equilibrium solvent chamber.
Another object of the invention is to provide an apparatus of the aforementioned character in which each equilibrium solvent chamber is individually sealed by a readily removable sealing membrane without the need to use any type of sealing oils or greases.
Another object of the invention is to provide an apparatus of the character described in the preceding paragraph in which each protein drop is stratigically supported within its solvent chamber on an upstanding column having an upper receptacle of optimum configuration to contain the particular protein drop.
A further object of the invention is to provide an apparatus for growing protein crystals in which the drop supporting columns and the equilibrium solvent chambers are integrally formed from a clear, non-wetting plastic having superior optical properties.
Still another object of the invention is to provide an apparatus of the class described which is easy to manipulate and stack and one which is readily usable with automated handling, pipetting and optical analysis equipment.
Another object of the invention is to provide an apparatus as described in the preceding paragraphs which can be inexpensively fabricated, thereby making it economically feasible to dispose of the apparatus after use.
Another object of the invention is to provide an apparatus for growing protein crystals in which the solvent is maintained within the equilibrium chambers of the device by a porous, foam material closely fitted into the equilibrium chambers.BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 is a generally perspective view of the apparatus of the present invention for growing protein crystals.
FIG. 2 is plan view of the apparatus.
FIG. 3 is a cross-sectional view taken along lines 3--3 of FIG. 2.
FIG. 4 is an enlarged fragmentary, cross-sectional view taken along lines 4--4 of FIG. 2.
FIG. 5 is a fragmentary view of a alternate embodiment of the apparatus of the present invention for growing protein crystals.
FIG. 6 is a greatly enlarged fragmentary crosssectional view taken along lines 6--6 of FIG. 5.DESCRIPTION OF THE INVENTION
Referring to the drawings, and particularly to FIGS. 1 through 4, one form of apparatus of the present invention for growing protein crystals is there illustrated and generally designated by the numeral 12. In this embodiment of the invention, the apparatus comprises interconnected side and end walls 14 and 16 respectively, which are integrally formed to define an interior enclosure 18. Each of the end and side walls terminate in a top wall, or edge, 14a and 16a respectively, which are disposed within a first plane. Formed interiorly of the side and end walls are a plurality of equilibrating solution reservoirs 20. As best seen by referring to FIGS. 3 and 4, each of the equilibrating solution reservoirs 20 includes a bottom wall 22 and a upstanding side wall 24 which terminates in an edge or top wall 26. Walls 24 are preferably generally cylindrical in shape. As indicated in FIG. 3, top walls 26 of each of the equilibrating solution reservoirs is, also, disposed within the first plane in a coplanar relationship with the top walls 14a and 16a of side and end walls 14 and 16.
Centrally located within each equilibrating solution reservoir 20 is an upstanding, generally cylindrically shaped column 28. Each column 28 includes, proximate the top thereof, a generally cup-shaped, protein solution carrying receptacle 30 having a wall terminating in an edge, or top wall, 32 which is disposed in a second plane spaced apart from the first plane of the top edges of the side and end walls. With this construction, top walls 32 are located substantially below the previously identified first plane.
A thin pliable cover 34, having an adhesive material on the lower surface thereof, is movable into sealable engagement with the top walls 14a and 16a of the apparatus and, also, into sealable engagement with the top walls 26 of each of the equilibrating solution reservoirs 20. With the cover 34 in position over the apparatus in the manner shown in FIGS. 3 and 4, each of the equilibrating solution reservoirs 20 is effectively sealed with respect to atmosphere. Cover 34 can be constructed of any suitable, thin plastic material, but a Mylar material having an adhesive on the lower surface thereof has proven quite satisfactory. Such a material is manufactured by the Corning Company and is sold under the name and style "Crack and Peel Mylar Sheeting."
In the preferred form of the apparatus, the side walls, end walls, equilibrating solution reservoirs and columns are all integrally formed by an injection molding process from a clear moldable plastic such as polystyrene. Other materials can be used to mold the apparatus but an optically clear, non-wetting plastic is, for reasons presently to be discussed, highly desirable. Further, the plastic material selected should be moldable in a manner such that the inner surfaces of the protein solution receptacles have a smooth texture.
In the embodiment of the invention shown in FIG. 1, the side and end walls, 14 and 16, are formed in a manner to provide a peripherally extending step 36 disposed between the upper and lower edges of the side and end walls 14 and 16. Such a step facilitates the stacking and handling of the apparatus of the invention during automated pipetting and crystal analysis steps.
In using the apparatus of the invention, each reservoir 20 is carefully filled with a selected equilibrating solution 38 so that the solution is contained within the annular shape defined by the side and bottom walls of each reservoir and the upstanding column 28 (FIGS. 3 and 4). It is to be observed that different equilibrating solutions can be added to each of the reservoirs 20 if this is desired. Typically, polyethylene glycol in high concentration (10 to 15%), or ammonium sulfate, is used as the precipitating agent in the equilibrating solution.
Following the addition of the equilibrating solution 38 to the reservoirs 20, a selected protein drop 40 is deposited within the receptacle 30 of each of the upstanding columns 20. The protein drop is typically 10 mg/ml of the protein in a buffered salt solution containing a lower concentration of the same precipitating agent used in the reservoir solution For example, the buffered salt solution may contain 5 to 7.5% polyethylene glycol. It is to be understood that the configuration of each of the receptacles 30 can be formed of an optimum size and shape to accommodate the particular protein drop being crystallized. For example, when lower surface tension solutions, including protein solutions containing detergents are used, a cup-shaped receptacle has proven satisfactory. Further, the texture of the walls of the receptacle can be varied to obtain optimum results for the particular protein solution being crystallized. In all cases, the non-wetting characteristic of the polystyrene plastic material from which the receptacle is formed significantly aids in the formation of protein solution drops of optimum configuration.
It is to be understood that the equilibrating solution as well as the protein drops can be added to the apparatus either by hand or by sophisticated automated pipetting apparatus which is readily commercially available. Because of the novel construction of the apparatus and the systematic placement of the reservoirs and protein drop receptacles, the apparatus is readily adaptable to most commercially available pipetting systems.
Once the equilibrating solution and protein drops have been added to the apparatus in the manner shown in FIGS. 3 and 4, the cover means 34 is carefully placed over the apparatus so that the top walls of the sides and edges of the apparatus as well as the top walls of each of the equilibrating solution reservoirs are positively sealed relative to atmosphere. With this arrangement, it is apparent that each protein drop is positively sealed within each reservoir 20 and can equilibrate against the particular equilibration solution which was earlier deposited into the particular reservoir. Since the starting concentration of precipitating agent is always higher in the reservoir than in the protein drop, once the chamber is sealed, water will diffuse from the protein drop to the reservoir until the concentration of precipitating agent at equilibrium is the same in the drop as in the reservoir. This diffusion results in a controlled steady increase in the concentration of both the protein and precipitating agent within the drop which forces the protein to come out of solution, hopefully as a crystal.
Following crystallization of the protein drops, the crystals can be analyzed within the apparatus, or after removal of the cover 34, the crystals can be removed from the apparatus for analysis in any appropriate manner.
Referring now to FIGS. 5 and 6, an alternate form of the apparatus of the present invention is there illustrated. In this embodiment of the invention, the construction of the basic apparatus is identical to that previously described and like numerals have been used to identify like components. As best seen in FIG. 6, in this second embodiment of the invention, porous means shown here in the form of a porous plastic material is placed within each of the equilibrating solution reservoirs 20. This plastic material, identified in the drawings by the numeral 44, is preferably formed in a generally annular shaped configuration which can be placed around columns 28 in the manner shown in FIG. 6. This porous, equilibrating solution absorbing means rests within each equilibrating reservoir in the manner shown in the drawings and is of porosity such that the equilibrating solution is substantially absorbed into the porous, or sponge-like plastic material, so that the apparatus can be conveniently transported without any danger of the equilibrating solution splashing into the receptacles 30 which contain the protein drops 40. Various types of plastic material can be used to form members 44, but a porous plastic material of a character similar to that manufactured by the Dow Chemical Company and sold under the name and style, STYROFOAM, has proven satisfactory.
The apparatus of the alternate form of the invention is used in much the same manner as the embodiment of the invention earlier described. However, in this instance, prior to adding the equilibrating solution to the apparatus, the annular shaped sponge-like members 44 are placed within each of the equilibrating solution reservoirs. Once members 44 are in place, the equilibrating solution is added to the reservoir in a quantity which can be readily absorbed by the porous means 44. The use of members 44 permits the apparatus to be filled with the reservoir solution prior to shipment to the end user.
As illustrated in FIG. 6, individual units of the apparatus of the invention can be conveniently stacked one on top of another following the addition of the equilibrating solution and protein drops and the sealing of the apparatus by the cover means 34. The step design of the apparatus is such a to enable a number of devices to be stacked one upon another in a highly stable configuration. As indicated in FIG. 6, because of the step construction of the end and side walls, a lower flange portion 14b and 16b of the side and end walls overlaps the adjacent apparatus in the manner illustrated in FIG. 6 thereby adding stability to the stacked units.
Having now described the invention in detail in accordance with the requirements of the patent statutes, those skilled in this art will have no difficulty in making changes and modifications in the individual parts or their relative assembly in order to meet specific requirements or conditions. Such changes and modifications may be made without departing from the scope and spirit of the invention, as set forth in the following claims.
1. An apparatus for growing protein crystals comprising:
- (a) a plurality of equilibrating solution reservoirs each having a bottom wall and an inclosing side wall so fixed in said apparatus as to terminate in a top disposed in a first plane;
- (b) a drop supporting means for supporting a protein drop disposed within each of said equilibrating solution reservoirs, each said drop supporting means comprising an upstanding column spaced apart from said side wall and having a protein solution depression disposed on the top thereof, the top of said protein solution depression being located below and parallel said first plane; and
- (c) cover means for engagement with said top of said equilibrating solution reservoirs for sealing said reservoirs wherein each of said protein solution depressions is in communication only with said equilibrating reservoir within which it is disposed.
2. An apparatus as defined in claim 1 in which said protein solution receptacle is generally cup shaped and formed from a plastic material.
3. An apparatus as defined in claim 1 in which said cover means comprises a thin sheet of transparent material provided with an adhesive for sealable engagement with said top of each of said equilibrating solution reservoirs.
4. An apparatus as defined in claim 1 in which each of said equilibrating solution reservoirs is circular in cross-section.
5. An apparatus as defined in claim 4 in which each of said columns is generally circular in cross-section.
6. An apparatus as defined in claim 5 in which said bottom wall of each of said equilibrating solution reservoirs is formed with said column by molding.
7. An apparatus for growing protein crystals, comprising:
- (a) interconnected side and end walls defining an enclosure, said walls terminating in a top edge disposed within a first plane;
- (b) a plurality of equilibrating solution reservoirs formed interiorly of said enclosure; each said reservoir including a bottom wall and an upstanding inclosing side wall terminating in an edge disposed within said first plane;
- (c) an upstanding column disposed within each of said equilibrating solution reservoirs, each said column having a generally cup shaped protein solution carrying receptacle having a wall terminating in an edge disposed in a second plane, said second plane being spaced below said first plane and parallel to said first plane; and,
- (d) a sealable cover for engagement with said edges of said side and end walls and for engagement with said edges of each of said reservoirs wherein each said protein solution carrying receptacle is in communication only with said equilibrating reservoir within which it is disposed.
8. An apparatus as defined in claim 7 in which each said column is integrally formed with said bottom wall of said reservoir within which said column is disposed.
9. An apparatus as defined in claim 7 in which said side walls, said end walls, said equilibrating solution reservoirs and said columns are integrally formed from a polystyrene.
10. An apparatus as defined in claim 7 in which each of said side and edge walls includes an expansion away from respective side and edge walls, the tops of each said expansion disposed a third plane spaced below said first plane so more than one of said apparatus can be placed in stackable arrangement with the bottom edges of said expansion of the upper apparatus of a stack being in surrounding contact with the top of said side and end walls of the next lower apparatus.
11. An apparatus as defined in claim 7 further including porous means disposed within each said equilibrating solution reservoir for absorbing the equilibrating solution.
12. An apparatus as defined in claim 11 in which said porous means comprises annular shaped members constructed from a porous plastic foam.
|January 1945
|Van Tuyl
|March 1972
|May 15, 1979
- Auduyeusky et al., "Technological Experiments Aboard Salyut-5", ACTA Astronautica, vol. 7, pp. 867-876.
Type: Grant
Filed: Aug 2, 1990
Date of Patent: Mar 17, 1992
Inventors: Alexander McPherson (Riverside, CA), Daniel Morris (Riverside, CA)
Primary Examiner: Gary P. Straub
Attorney: Edward S. Irons
Application Number: 7/563,670
International Classification: B01D 902; C30B 3500;


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Parish Life
About the United Church of Christ
Foyer Groups
Members and friends join for casual fellowship and refreshing meals. This is a great time to get to know one another outside of worship.
First Friday Hop
During the months of April through December, we are open on the First Friday of each month for those wishing to appreciate our beautiful sanctuary. We often have art exhibits in the Narthex, or musical guests in the Sanctuary, and on our grounds. Our hosts are eager to share our history and answer any questions visitors may have.
Lemonade Social
From May through September, our fellowship continues after worship each Sunday, weather permitting, as we gather in the Courtyard to share cookies and lemonade.
Shakespeare in the Park
We gather annually in Old Louisville's Central Park to picnic and enjoy a fabulous performance of the oldest free Shakespeare festival in the United States.
Operation Brightside
Operation Brightside is a division of the City of Louisville focusing on sustainability through beautification. Participants from St. John gather annually on a designated Saturday morning to clean up our campus, streets, and surrounding neighborhood. We also manage to include fun and fellowship!
The Book Group
The Book Group includes both members and friends of St. John. They meet in the Parish House parlor one morning each month to discuss themes and opinions about the book they are currently reading. Fellowship often extends into lunch afterward.
Annual Fish Fry
During the Lenten season, we traditionally share a meal at our annual Fish Fry. It is one of many meals after worship we enjoy in the Parish Hall throughout the year.
Annual Pancake Breakfast
On the Sunday before Ash Wednesday, St. John hosts our Annual Pancake Breakfast. It is our last ecclesiastical indulgence before the beginning of Lent.
NuLu Festival
Embracing our neighborhood, we open our doors for the annual NuLu Festival each September. Visit our booth to learn more about St. John, the United Church of Christ, and the availability of our campus as a wedding venue. The sanctuary is open for you to appreciate the artistry of our stained-glass, the architecture, and to learn a bit of our 170-year history.
Easter Celebrations
We begin our Easter celebrations with our annual Easter Breakfast in the Parish Hall, which is followed by a Easter egg hunt in the Courtyard and grounds. This usually features a certain 'special visitor.' Our most capable Organist-Choirmaster expands our Holy Communion Service with an inspiring Easter Concert Prelude – each year surpassing the past one.
Hat Sunday
Following one of Kentucky's longest traditions, and as part of Louisville's largest festival, Hat Sunday falls on the Sunday after the Kentucky Derby. Women and men both are encouraged to join in the celebration.
Wear Red Sunday
In observance of the long tradition of representing the Holy Spirit with the liturgical color red, we enjoy wearing red on Pentecost Sunday.
Thunder Over Louisville
Three weeks before the Kentucky Derby, Thunder Over Louisville kicks off the Derby Festival with the largest annual fireworks show in the country. Coupled with a major air show, it draws well over one hundred thousand to Louisville's waterfront parks. Cars are parked in our lot as an annual fundraiser for the church.
Summer Picnic
Whether it's hamburgers or brats, German or American potato salad, cakes or pies, you can find it at our annual Summer Picnic. Each August we gather after worship for a cookout, a potluck, and good company.
Blessing of the Animals Service
Our four-legged, furry, feathered, and scaled family members can all be blessed at the Blessing of the Animals Services held on the first Sunday of October in the Courtyard. Church members, friends, and neighbors all share in the service.
Thanksgiving Luncheon
Every Sunday before Thanksgiving we gather after worship in the Parish Hall in gratitude and fellowship for our Thanksgiving dinner with each other. Everyone contributes a covered dish to brighten the traditional fare.
Children-Youth Christmas Pageant
As Christmas approaches we depart from our traditional service and turn to a form with Medieval roots. With the children all dressed in their part, readings of the Bible alternate with familiar carols as the children act out the Christmas Story. It is a favorite service of some.
Brooklawn/Uspiritus Christmas Wish List
Originally named the German Protestant Orphan's Asylum when it was founded in 1852 by German immigrants, it would bear several titles [Protestant Orphan Home (1940's), Brooklawn Children's Home (1960's)] before its merger with Bellewood Home for Children in 2012 to form Uspiritus. St. John contributes spiritually, materially, and financially to their efforts. Christmas time brings special needs that beg to be filled.


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Masters & champions. Use the best shortcuts of masters and millionaires, the best shortcuts of champions and billionaires,
Shortcuts are their secret.
Shortcuts accelerate everything.
Thus, the Psychology of Shortcuts is.
Shortcuts are a hugely untapped resource.
Using more of what you already have generates more.
Repeat it one hundred times, faster, and see for yourself.
Shortcuts are the best tools for accelerating any desired result.
Ever buy food prepared by someone else? Is this not a shortcut?
Ever ask someone to do something that you could've done yourself?
Have you ever performed two totally separate tasks, with skill, at the same time?
These are shortcuts you've used countless times before, that' you're still using daily.
Every one of us uses shortcuts. The smarter you get, the more shortcuts you figure out.
Sometimes, the best shortcut of all is to find someone else already using great shortcuts.
By simply mimicking their words, actions, even their attitudes and physical postures,
you are indubitably certain to repeatedly duplicate or near-duplicate their results.
It's not the art of success, it's the replicable science of success; a recipe.
When you follow the recipe you obtain identical or similar results.
Rather than focusing on food recipes, here's it about success.
the Psychology of Shortcuts of masters and millionaires,
because even you deserve a big taste of success.
the ; Psychology of Shortcuts urges you on,
in hope that you'll use your new wealth,
intelligently, and compassionately,
because it all comes back to us:
good, bad, and indifferent.
Shortcuts, easing life.
YOUR shortcuts.
Learn more in order to live more, at the Psychology of Shortcuts.
Crafted with devotion to your potential, by your Godfather of EyeCandy, for You
the secrets that are YOUR best shortcuts to succeeding, proven to work by rafters of masters.
Enjoy, the Psychology of Shortcuts is free for your life, except half of your new wealth.
That belongs to MisterShortcut, who instructs you to invest it into helping the helpless.
Each birthday, put a hundred into something not to be touched for a century or two.
The growth of interest generates more millions than you will likely earn in your life.
As long as we train humans to be greedy, there will be kids starving to death.
Those hundreds, invested once per year, will feed many millions of children.
Never mind what your neighbor is doing. Invest in your own immortality.
Use the best shortcuts of masters and millionaires, the best shortcuts of champions and billionaires,


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Restaurant Manager
Wyoming, MI 49519
Job Description
We are seeking a Restaurant Manager to join our leadership team.
Restaurant Manager Responsibilities:
- In charge of Full-service Restaurant Operations
- Help direct systems and processes for team efficiency
- Able to work in unity with other members of the supervisory team
- Ensure excellent customer service and great quality food
- Creative operator to support company culture
Restaurant Manager Requirements:
- 2-4 years of Restaurant Management Experience
- Strong leadership and communication skills
- Good understanding of FOH operations
- ServSafe certified a plus
- Good knowledge and understanding of craft beers
Restaurant Manager Benefits
- Excellent Starting Salary, ranging from $45,000 to $50,000, based on experience
- Progressive Health Insurance Package
- PTO, Company Values Quality of Life Balance
Job Requirements
Recruiter Managing this Job
Larry Chesner
Senior Recruiter
Recruiting is awesome! It enables me to stay close to the hospitality business (which I love), serving both my guests and clients. I enjoy that wonderful feeling of knowing you have provided excellent, above-and-beyond service.
- Larry Chesner joined Goodwin Recruiting in March of 2017. He grew up in New Jersey as a latch-key kid with two working parents and thus learned to cook at an early age.
- Larry went on to attend Johnson and Wales College in Providence, RI. He earned an Associate's degree in Culinary Arts and a Bachelor's degree of Science in Food Service Administration.
- Much of Larry's career was with Interstate Hotels, where he started in F&B, then migrated to rooms operations and General Manager. He helped open several properties during his tenure and worked in Boston, MA; Providence, RI; Corpus Christi, TX; Cincinnati, OH; Philadelphia, PA and Grand Rapids, MI. This included several brands like Marriott, Hilton, Holiday Inn, Wyndham, and independents. Larry also worked for Wyndham Corporation, Lane Hospitality, and National Hospitality Services as an Operations Manager and General Manager.
- Larry has been married for 23 years to Cherie. They have two daughters, Sam (19) and Sarah (15). Larry is a classic foodie; he loves to cook, drink, and learn about food and wine. He enjoys the outdoors, fishing, (crabbing when he gets back to NJ for visits), hiking, and camping. Larry's favorite nonprofit is "Give Kids the World."


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We are looking for the best of the best
Can you combine rail and rolling stock working knowledge and experience with a professional consultancy project delivery focus? Do you have a proven track record in rail – ideally in rolling stock operational and commercial engineering? Are you looking for challenging and exciting business-critical roles in vital railway projects and operations? Are you looking for opportunities to apply your knowledge and experience more widely to the cutting edge issues of our modern railway?
Join our exciting journey and help to shape our growing business.
We believe that our blend of railway rolling stock industry experience and practical, diligent, hands-on consultancy delivered collaboratively within the client team has proved a winning formula for our success to date. We help clients by bringing real operational knowledge and experience and professional assurance and delivery to solving their varied problems.
Our culture is very important to us so it is essential that we find consultants who are the right 'fit'. A great attitude and a desire to excel are vital, as is being a team player – all built on a rail / rolling stock and engineering background. Our people are the best of the best and embody our values of integrity, professionalism and excellence. We believe that approach, actions and delivery speak louder than words.


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Connection Groups at Christ Church are small groups who gather together to grow spiritually, pray faithfully and serve compassionately together in order that they may increase their capacity to love God above all things and their neighbors as themselves.
Our small groups are one of the primary places where authentic community is experienced at Christ Church. Groups gather to share in the journey of faith. Moreover, these groups provide respite from the anxieties and pressures of life in New York City, as friendships are deepened and individuals strengthened in community.
Prayer Partners– Everyone is welcome to join us as we pray over the prayer requests we receive weekly. Because Christ said, "For where two or three are gathered in my name, I am there among them," we believe that in our joining together in prayer, Christ hears our requests. Pray with us. Thursdays, 12:30 p.m., Christ Church Prayer Line.
LGBTQ Connection Group – Join other LGBTQ members and friends to meet in the evening monthly to discuss a topical book relating to the LGBTQ community and our spiritual lives.
New Parents Connection Group – If you have a new bundle of joy and could use some connection and support with others also on this journey, this is the perfect Connection Group for you. This upcoming group will meet once a month on Sunday during the 10:00 a.m. hour so that childcare can be provided.
To join any of these groups or to find out more information, contact us here.


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Friday NOLA Wrap-Up
Surprisingly, the Verizon IndyCar Series was able to get the second practice in. It was probably a smart move, as these conditions are probably going to be similar to what they will be racing in on Sunday. Fortunately, they were able to get most of session in with just a few harmless raindrops falling. I'm glad that we were able to see cars on track on our first day here.
The times were down from the first session with a wet track. Honda was able to salvage their day by putting two cars in the Top Five. Will Power led the session with a quick time of 1:20.17. Ryan Hunter-Reay was second. Simon Pagenaud and Stefano Coletti were third and fourth respectively, while Simona de Silvestro was fifth.
I was able to snap a few pictures while we were in the pits. We stood in James Jakes pit for a while catching up with friends; more on seeing the aero kits in person tomorrow. We then wandered the pits and caught Josef Newgarden and Luca Filippi about to go out. I also stole a quick shot (albeit of the back of his head) of one of my all-time favorites, Rick Mears, taking it all in from the Penske pits. Then we got to speak with our fellow Nashvillian, Newgarden, after practice as he was heading to the paddock.
That's going to do it for us for now. We're going to go eat some of the good cuisine I've been dreaming about since we made our reservations last August. Check back early and often tomorrow. Also, don't forget you can follow us both on Twitter; @Oilpressureblog and @MrsOilpressure. Thanks for reading, and we'll see you tomorrow.
George Phillips
3 Responses to "Friday NOLA Wrap-Up"
Awesome. So you like NOLA?!
Thanks for being our reporters on the ground. I had forgotten you two were attending NOLA.


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1 tablespoon paprika2 teaspoons pepper
1-1/2 teaspoons kosher salt
1-1/2 teaspoons brown sugar
1-1/2 teaspoons ground cumin
1-1/2 teaspoons chili powder
1 teaspoon sugar
1/4 teaspoon cayenne pepper
1 beef top sirloin steak (1-1/2 pounds)
Comments are closed.
All burgers made with beef & pork for the juiciest and most flavourful burger ever! We come plain too! Just ask!


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We leave Cape Town at 8am and head for the picturesque Hottentots Holland Mountain Range. We pass the beautiful town of Somerset West (and Gordon's Bay in view on the foothills of the mountain) and take on the awesome Sir Lowry's Pass from where we will have a stunning'view over False Bay, Cape Town and Table Mountain in the distance. A short coffee stop is taken on the mountain after driving along the beautiful apple orchards of 'Apple Country.'
The mountain pass of Houwhoek now follows, where after we descend and see the quaint little town of Botriver below and the outstretched wheat and canola fields in the distance. Next is the fisherman's town of Hawston and then we drive into the town of Hermanus for whale watching from the rocks. An orientation tour follows along the coastline before we stop on the square known for rock based whale watching. With so many restaurants and craft markets on the square the client can even grab a take-away lunch and watch the whales while enjoying it.
After lunch and enough time for whale watching we return via the panoramic False Bay Coastal Clarence Drive. Drive to Stoney Point to see the hundreds of African penguins in the water. Time permitting, we even visit the Harold Porter Botanical Gardens for a short hike. We then carry on with this spectacular drive and join the main highway to take us back to Cape Town. Arrival in Cape Town is at approximately 5pm.


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Thursday, 12 November 2009
Radio ga ga
A different day to my normal, that's if my days are ever normal! Boots the Chemist was the 1st port of call, I'm sure stress and nerves bring on my allergies! Driving to Carlisle to Radio Cumbria I was full of excitement and also that jitter feeling on a visit to the dentist! I was shown round the studio's which was quite a different view of the radio that I listen to. Then into the studio, starting to get a touch nervous now but Steven the radio wizz soon put me at ease, or nearly! 'Right Jane just read it out and lets see how we get on' ahhhhhhhhhh so with hands shaking and voice full of fear off I went. Guess what, it was great fun, and Steven Radio wizz seemed pleased and so the date for the 1st Jane Ward radio ga ga is Monday 23rd November 5.45 pm. Now doubt I'll keep you posted.
3 comments:
Well done Jane! By the way, I love the new slideshow that you've added at the side. Beautiful!
- 12 November 2009 at 21:50
- Dave from Keswick said...
Looking forward to hearing you on the radio. Good luck or should that be brake a leg ???
- 15 November 2009 at 23:28
- Lakeland art said...
Hey Dave, prefer good luck, no broken bones thank you!
- 16 November 2009 at 10:56
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ST. LOUIS — The Cardinals slumping bats remained quiet through a 95-minute rain delay. Once they were able to get going, they never cooled off.
Jose Martinez drove in five runs and had four hits to help the Cardinals rout the Philadelphia Phillies 12-4 on Friday night.
"We know it's not going to happen every day," Martinez said. "It's not going to happen often, but days like this give us a lot of confidence."
Francisco Pena homered and also had a single and double for the Cardinals.
The Cardinals set a season high with 15 hits, but got none off Phillies shortstop Pedro Florimon, who threw a perfect eighth inning in his second pitching appearance of the season. Florimon then hit his second home run of the season to cap off his night in the ninth.
Despite the margin of defeat, Phillies manager Gabe Kapler had plenty of praise for Florimon.
"What an inspiration he has been to our team," Kapler said. "I walked over to him and said 'Flo, I'd really like you to pitch this inning for us and he just looked at me and said 'Absolutely, I got you."'
Michael Wacha (5-1) gave up two runs on five hits. He struck out eight and walked two to earn his 50th career win.
Despite the rain delay, Wacha held his opponent to two or fewer runs for his sixth consecutive start.
"It truly is an art to being able to stay prepared," Cardinals manager Mike Matheny said. "I think he kind of figured that out by force."
Jake Arrieta (3-2) lasted three innings in his shortest outing of the season. He allowed four runs on five hits with two walks.
"I didn't do my job of establishing strikes early, which makes it harder for the guys coming in after me," Arrieta said, "Tough conditions, a delay, but you can't really use that as an excuse because those guys have to deal with the same circumstances. Just one to forget."
Martinez's four hits matched his career high set on April 12 at Cincinnati.
Pena had his second consecutive three hit game including his first home run of the season. He is hitting .360 since Yadier Molina was placed on the disabled list May 6.
"I'm not trying to do more. I'm not trying to put pressure on myself. I'm not trying to put my team on my back," Pena said. "My mentality is just winning ballgames and controlling the pitching staff."
Odubel Herrera extended his streak of reaching base to 44 consecutive games with a two-run single off Wacha in the fifth inning.
Home plate umpire David Rackley departed the game prior to the eighth inning. Second base umpire Hunter Wendelstedt replaced him.
Pena's home run made him and his father Tony the second father-son tandem in Cardinals history to hit home runs for the franchise. They join Ed and Scott Spezio in accomplishing that feat.
Phillies: C Jorge Alfaro departed the game in the middle of the fifth inning with right knee soreness.
Cardinals: SS Paul DeJong was placed on the 10-day disabled list with a fractured fifth metacarpal on his left hand. DeJong injured the hand after being hit by a pitch in the eighth inning of Thursday night's game. The team also placed RHP Matt Bowman on the 10-day disabled list with blisters on his right index and middle fingers. The club recalled INF Yairo Munoz and OF Tyler O'Neill from Triple-A Memphis.
Philadelphia RHP Zach Eflin (1-0, 0.71 ERA) will face St. Louis RHP John Gant (1-1, 4.15 ERA) in the third game of a four game series Saturday.
More AP baseball: https://apnews.com/tag/MLBbaseball


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It's probably not why you think, though.
Okay. Okay. Okay. I miiiiiiight get my proud mummy-face on every time she does something awesome, like hits the tennis ball perfectly with her little Wilson racket(it's so ridiculously cool that thing) or ride her skateboard like a baby pro. Even when she climbs the mountain side at the beach without any fear what so ever. Ohh, and by the way she can also swim by herself now. No biggie.
She's a little adventurer and is fairly advanced physically. Two things that I, admittedly, do cherish quite a lot. However, there is absolutely no doubt that I would love her just the same if she had had poor balance and couldn't climb a fallen tree.
No, what REALLY makes me proud of our little girl is that she's got the biggest and most caring heart and soul ever seen in such a tiny body. We have made a human being loaded with empathy. How rad is that?
Two of the last three weekends I've been ridiculously sick. You know, stomach bug sickness, of the kind where you lay on the bathroom floor at 3 a.m. thinking you most definitely is dying. Nothing is supposed to be that awful and painful and disgusting. One night I even had Josh call an ambulance, when I REALLY thought I was dying. My hole body was literally cramping. That's what happens just before you die, right?!! Well, it turned out I had just been hyperventilating for too long, due to all the vomiting, and that, apparently, causes the body to cramp. So yeah, I survived. Twice.
The following days I was very weak and tired, and that's when Abby stepped her empathy game right up. It's nothing new that she does these things, but this time I really noticed it and appreciated it. I might even have shed an emotional tear in there in my sickbed, when she came in and did nothing but look at me and kiss my hand. Kisses make everything better.
Sadly, kisses aren't enough to cure real sickness, so she also brought me cucumber and biscuits and plenty of water. I could even have some from her drink bottle if I liked. Nothing out of the ordinary, really. What makes it special is the look on her face in those situations. There is no doubt in the world, that it hurts her deep inside to witness someone else in pain. It makes her feel terrible and she will do anything to help and "make it aaaaaaaall better".
Also, her ability to switch from a terrorizing toddler to a caring and helpful little girl, as soon as she realizes someone else is not okay, is amazing to witness – and every time it happens, I get confirmed that, despite her tantrums and crazy fits, she is pretty much emotionally on track. Such a relief.
During the week, I have daily been met with a "Good morning, are you better, mummy?" Today I could finally tell her, that I'm back to normal. That made her very happy…
Now, the above is just one tiny example of all the times where this little person over floats with care and emotions for other people.
After my parents left for Denmark, I was an emotional wreck and I could and would not hide from Abby, that I was very sad. Abby was also sad those days, but she assured me, that it would all be okay, and we would soon fly to mormor's house on a big aeroplane.
She also gets very upset, when she can hear the neighbour boy cry, or any other child cry, for that matter. She wants to help them out, because "no-one is supposed to be sad".
Today, Josh had a migraine. Abby went and found the thermometer all by herself and gave it to him, because that's what you need, when you're sick, right? "For your fever, daddy. It will make you better."
The list could go on and on, but I think my point is clear by now; Abby is an awesome human being and I could not be any more proud of her for it.
Maybe it's natural for children to have and show this amount of empathy. Maybe it's the surrounding environment that ruins it on their journey towards grown-up-ness. If so – shame on the environment.
I know it is not a matter of course for grown-ups to have so much empathy, none-the-less to show it and put it into action like Abby does. I, for sure, don't consider myself to be very good a showing empathy, but I do try my best to break down my emotional barriers, and even I, at the age of 33, is learning to be a better person these days. And Abby's the best teacher I could get!
So yeah, we'll worry about learning to count to 10 later. For the time being we focus on the important things around here. Such as showing empathy – and getting those 10.000 hours of tennis practice in the book.


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being able to delete the stupid thing


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Black & Decker 1200W Lawnmower, 32cm Cut
Item number MGAWE
The Black & Decker 1200W Lawnmower makes easy work of keeping your garden trim.The bike-style handle is compact and lightweight, giving you optimum control and manoeuvrability.. The high performance blade delivers 80% better grass collection and greatly improves cutting results.
A large 35-litre grass box lets you power through the mowing without the frustration of repeatedly stopping to empty the container. The full box indicator alerts you when it's finally time to empty.
With improved wheel design for better grip and control, an integrated carry handle as well as 3 height adjustable settings, this Black & Decker mower has everything you need for a flawless garden.