text:
MILL CREEK — People in affluent enclaves around Mill Creek are fixing to offer on-the-cheap deals for Saturday.
The community garage sale is set to span 21 neighborhoods around the golf course.
"It starts at Aspen and ends at Winslow," said Mary Ann Heine of the Mill Creek Community Association. "It's quite an adventure."
Bargain hunters can find high-end items for a steal. Those who start early, at around 7 a.m., are likely to score the best wares.
The daylong event is not advertised and there isn't an official organizer.
"It happens on its own, believe it or not," said Mike Todd, a longtime city councilman and former mayor. "In fact, it can't be stopped."
People have long been peddling their possessions in front of homes and in garages throughout the town. The tradition takes place on the first Saturday in May and again in October.
Heine said she believes Mill Creek was the first in the area to hold a large-scale garage sale. It's been going on since before Mill Creek became a city in 1983.
Heine said she believes one of the association's original board members came up with the idea for the sale. The powerful homeowners group, which now includes more than half the city's population, organized in 1973.
"Before we were here, there was nothing here," Heine said.
In the early 1980s, Mill Creek had about 3,000 residents. The garage sale began around that time on Village Green Drive, in the association's core territory.
The group bans homeowners from posting signs to advertise.
Todd said he believes the garage sale came about as a response to the strong controls. Holding a number of yard sales on the same day also makes for a better turnout, he said.
"It gets the mess and fuss over with," Heine said.
The association wanted homeowners to be able to have the sales but in an organized way, she said. It now ignores sign violations on sale day.
At least half of the group's 10,000 residents, are setting up to take part this year, Heine said.
"There are incredible things you can buy here," she said. "We have a really interesting neighborhood where people have been to a lot of places around the world."
Mill Creek boasts a median household income of just under $90,000 a year. An average home costs $415,000, according to census data.
Many community groups get in on the action as a way of fundraising.
Goodwill brings donation trailers to the association's parking lot. After the sale, homeowners can get rid of items that didn't move.
"They find it's one of the best pickups all year," Heine said.
The garage sale used to be a weekend-long ordeal. The homeowners group several years ago put its foot down to limit the event to Saturday only. Some rallied to bring the two-day sale back but others were happy setting up once, Todd said.
Over the years, the city has attempted to control traffic and parking problems that result from deal-seekers flooding the area. Todd said the efforts were costly and cumbersome. They also weren't enforceable.
The city reined in promotion of the event. Todd said the traffic issues have since faded from nearly impossible to very busy.
Mayor Pam Pruitt said the event brings hundreds of people into the city. The Mill Creek resident since 1980 said she believes the garage sale is the largest in the area.
"You can pick up absolute bargains," she said. "It's the day you walk out of your house and the mall is the street in front of you."
Amy Nile: 425-339-3192; email@example.com.
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text:
This site is the archived OWASP Foundation Wiki and is no longer accepting Account Requests.
To view the new OWASP Foundation website, please visit https://owasp.org
This OWASP Chapter is inactive. If you are interested in restarting this Chapter contact us for more information or apply to restart this chapter .
- 1 OWASP Bhubaneswar
- 2 Participation
- 3 Sponsorship/Membership
- 4 Join OWASP Bhubaneswar via our Meetup Group
- 5 Chapter Sponsors
- 6 Local News
- 7 Chapter Meetings
- 8 Bhubaneswar Chapter Founders
- 9 Bhubaneswar Committee Members
OWASP Bhubaneswar
Welcome to the Bhubaneswar chapter homepage. The chapter leader is Somen Das
OWASP Foundation (Overview Slides) is a professional association of global members and is open to anyone interested in learning more about software security. Local chapters are run independently and guided by the Chapter_Leader_Handbook. As a 501(c)(3) non-profit professional association your support and sponsorship of any meeting venue and/or refreshments is tax-deductible. Financial contributions should only be made online using the authorized online chapter donation button. To be a SPEAKER at ANY OWASP Chapter in the world simply review the speaker agreement and then contact the local chapter leader with details of what OWASP PROJECT, independent research or related software security topic you would like to present on.
to this chapter or become a local chapter supporter. Or consider the value of Individual, Corporate, or Academic Supporter membership. Ready to become a member?
Join OWASP Bhubaneswar via our Meetup Group
From Feb 2014, we have moved to a Meetup group. MeetUp.com will replace the traditional subscriber email list eventually (referenced above in the top of this page). It allows us to better communicate with members, RSVP for events, and announce meetings - all in one place. Click on the following link to visit our meetup page and signup.
Chapter Sponsors
Sponsor OWASP Bhubaneswar Chapter to help us organize open and free monthly meetings.
Local News
Welcome Message
To know what OWASP Bhubaneswar Chapter has to offer & the detail information about becoming a OWASP individual/corporate member, access the following template :
OWASP Membership Flyer Bhubaneswar Chapter
- Note: Everyone is welcome to join us at our chapter meetings !
Chapter Meetings
OWASP BBSR Chapter Meeting - February 2014
Meeting date & Location
Date: 28th of Feb 2014
Location: College of Engineering & Technology, Bhubaneswar- 751003, Odisha, India
Time: 1:00PM - 5:00PM
Program Details for 28th Feb 2014
Event Presentations
Web Application Security Educational Program
Event Pics
Here we have some pictures of the event.
OWASP BBSR Chapter Meeting - October 2012
Meeting date & Location
12th of Oct 2012
Auditorium, KOEL Campus, KIIT University - 751024
Time: 11:00AM - 02:00PM
OWASP Bhubaneswar Chapter Meeting 12 October 2012
OWASP November 2011 Meeting
Meeting date & Location
16th of Nov 2011
Tata Consultancy Services, Kalinga Park, Special Economic Zone, Bhubaneswar
Time: 5:00PM - 7:00PM
Event Pics
Event Pictures
Event Presentations
OWASP Top 10 Presentation
OWASP Introduction & Bhubaneswar Local Chapter Kickoff Meeting
Meeting date & Location
6th Sept 2011
Enterprise System Solutions (P) Ltd. ( Conference hall 1 ), Near Infocity, Bhubaneswar - 751024
Time: 5:30PM - 7:30PMEvent Pics
Meeting Pictures
Event Presentations
Bhubaneswar Local Chapter Kickoff Meeting
Bhubaneswar Chapter Founders
- Chapter-Leader - SOMEN DAS
- Co-Chapter Leader - Srimant Acharya / Srimant Acharya
Bhubaneswar Committee Members
- Speaker Coordination Committee (SCC) -
Anshuman(Leader) & Jyoti Acharya(Leader)
- Participant Coordination Committee (PCC) -
Rashmi Tarai (Leader) & Bijay Sethi(Leader)
- Venue Coordination Committee (VCC) -
Sanjib Kumar Sahoo (Leader)

subset: commoncrawl


text:
Elegant solutions for complex IT problemsDDBC tackles extremely complex database and systems integration projects, provides thoughtful, tested solutions on time and on or under budget, and invariably exceeds the expectations of our clients.
The abilities of our small, nimble, and highly-qualified team of consultants rival even the largest players in the IT consulting space, and we do so at a lower cost. We do this by hiring exceptional talent, following time-tested processes and methodologies, and bringing our vast experience and value-driven philosophy to bear on every project.
|Legal and Privacy Notice


text: The Virginia Residential Landlord and Tenant Act (VRLTA) handbook has been prepared to provide information on the rights, remedies and responsibilities of landlords and renters concerning the rental process. Before signing a lease, prospective tenants should read and understand the terms of the contract. Consulting a lawyer or the landlord for clarification of the rental agreement is advisable.


text:
Feelin' It
Quelle: Spotify
[Produced by Ski Beatz]
[Hook: Mecca]
I'm feeling it, fill the glass to the top with Moet
Feeling it, feel the Lex' pushing up on the set
I'm feeling it, feel the high that you get from the la
Feeling it, if you feel it raise your L in the sky
[Verse 1]
I keep it realer than most, I know you feeling it
Cristal on ice, I like to toast, I keep on spilling it
Bone crushers I keep real close, I got the skill for this
On my back the flyest clothes, looking ill as shit
Transactions illegitimate cause life is still a bitch
And then you die, but for now, life, close your eyes and feel this dick
Since diapers, had nothing to live for like them lifers but
Making sure every nigga stay rich within my cipher
We paid the price to circle us, success – they turned my mic up
I'm 'bout to hit these niggas with some shit that'll light ya life up
If every nigga in your clique is rich, your clique is rugged
Nobody will fall cause everyone will be each others crutches
I hope you fools choose to listen, I drop jewels, bust it
These are the rules I follow in my life, you gotta love it
Jiggy-Jigga looking gully in the joint
If y'all niggas ain't talking 'bout large money, what's the point?
[Verse 2]
Even if it ain't sunny, hey, I ain't complaining
I'm in the rain doing a buck 40, hydroplaning
What, shorty? (Where you disappear to, son?) Maintaining
Putting myself in a position most of these rappers ain't in, I'm living
The ill streets' blues got you hunger panging
Nothing to gain and a whole lot to lose you still singing, fool
I'm thorough, in every borough my name be ringing
Warming it up for the perfect time to hit your brain, and
Ya feelin' it? To all the girls that bought a girdle to conceal my bricks
No doubt, they can vouch my life is real as shit
'95 South and Papi on the Hill and shit
And all the towns like Cambridge that I killed with, shit
And all the thorough-ass niggas that I hustle with
Throw your joints in the air one time and bust your shit
These fake rappers can't really know I'm loving it, you feeling it?
[Verse 3]
What, y'all ain't heard that nigga Jay hot?
The Cristals they keep me wet like Baywatch
I keep it tight for all the nights my momma prayed I'd stop
Said she had dreams that snipers hit me with a fatal shot
Those nightmares, ma, those dreams that you say you've got
Give me the chills but these mills, well, they make me hot
Y'all feel me, enough to stop the illing right?
But at the same time these dimes keep me feeling tight
I'm so confused, okay I'm getting weeded now
I know I contradicted myself, look, I don't need that now
It's just once in a blue when there's nothing to do
And the tension gets too thick for my sober mind to cut through
I get to zoning: me and this chick on the L and then we're boning
I free my mind sometimes I hear myself moaning
Take one more toke and I leave that weed alone, man
Zeige deinen Freunden, dass dir Feelin' It von JAY Z gefällt:


text:
This week in new hires, New York Life realigned its practice groups and named new team leaders, and also named Sue Ericksen as CIO; Sen. Max Baucus praised Senate approval of three tax court nominees for second terms; and Great-West Retirement Services promoted Brent Neese.
Also, Essex Investment Management welcomed Gregory Goostray; Shefali Desai returned to MassMutual; and linkedFA brought in Eric Levine.
New York Life Retirement Plan Services Realigns Practice Groups, Picks 5 to Lead Initiative
New York Life Retirement Plan Services announced Tuesday a new service model to align service team practice groups into four industry segments: Taft-Hartley, technology, finance and professional services, and manufacturing, materials and retail. Each practice will have its own team of relationship managers, communications consultants, investment specialists and ERISA consultants. The company named five at the firm to lead the initiative.
Joan Driscoll, a 24-year industry veteran with 18 years of experience at New York Life, has been promoted to lead the new effort as managing director of client strategy, and reports to David Castellani, CEO. James Byrne is director and retirement practice lead for Taft-Hartley; Joseph DeStefano is director and retirement practice lead for financial and professional services; Scott Francolini is director and retirement practice lead for manufacturing, materials and retail; and Michelle Morey is director and retirement practice lead for technology. They will now report to Driscoll.
New York Life Names Sue Ericksen Chief Information Officer
New York Life Insurance Company announced September 22 that Senior Vice President Sue Ericksen has been named chief information officer. In this role, she will report to Frank Boccio, executive vice president and chief administrative officer, direct a staff of more than 1,000 technology professionals, and set the company's strategic technology direction as leader of the company's corporate information department.
Ericksen, who replaces Eileen Slevin, joined New York Life in 2009 as chief technology officer and senior vice president responsible for New York Life's information technology vision, strategy and planning, overseeing the enterprise architecture and engineering division in the corporate information department. Prior to that, she served as chief technology officer at Merrill Lynch Banks; before that, she held a number of executive positions at Citigroup, including chief technology officer for CitiCards and chief information officer for CitiFinancial.
Sen. Baucus Lauds Senate Approval of Tax Court Nominees
Senate Finance Committee Chairman Max Baucus, D-Mont., on Tuesday praised the Senate's unanimous approval of the nominations of Juan Vasquez, Maurice Foley and Joseph Gale to serve second terms as judges of the U.S. tax court, which hears a variety of tax law-related cases and gives taxpayers a dedicated venue in which to dispute IRS claims. Baucus voiced support for Vasquez and Foley at a July 28 Finance Committee hearing and for Gale at a September 12 hearing. The Committee approved the nominations September 20; all were originally appointed to the U.S. tax court by President Clinton in 1995.
Vasquez is also an adjunct professor at the University of Houston Law Center. Foley previously served as deputy tax legislative counsel in the Treasury's Office of Tax Policy and as an attorney-advisor for the IRS. Gale served as staff director and chief tax counsel on the Senate Finance Committee immediately prior to his initial appointment to the tax court, and advised Sen. Daniel Patrick Moynihan, D-N.Y., on tax issues between 1985 and 1992.
Great-West Retirement Services Promotes Brent Neese
Great-West Retirement Services announced September 22 that it has promoted Brent Neese to vice president, government markets. In this role, Neese will be responsible for sales, renewals, market strategy, field operations and plan management for the organization's government clients. He is based in Great-West's headquarters in metro Denver and reports to Gregg Seller, senior vice president of government markets.
Neese joined Great-West Retirement Services in 2007 as regional vice president, eastern region, pursuing growth in the organization's government markets business across the eastern U.S. Prior to joining Great-West, Neese served in a number of management and sales positions with AIG Retirement Services for more than 12 years.
Essex Investment Management Welcomes Gregory Goostray
Essex Investment Management Company, LLC, announced September 22 that Gregory J. Goostray has joined the team as vice president, sales and marketing. In this role, Goostray will focus on identifying and developing relationships with intuitional investors, RIAs, multifamily offices and investment consultants, as well as working with current institutional and private clients.
Goostray has over 18 years of industry experience in sales and relationship management, most recently as senior national accounts manager for the intermediary business group at State Street Global Advisors (SSgA). Prior to that, he was a wholesaler for SSgA's advisor strategies group.
MassMutual Retirement Services Hires Shefali Desai as National Sales Leader for Emerging Market
MassMutual's retirement services division announced Wednesday that Shefali Desai was appointed national sales leader of emerging markets, effective September 6. In this role, Desai leads the division's 15 sales directors as well as third-party administrator (TPA) channel managers targeting small market retirement plans. Desai reports to Scott Buffington, national sales manager for the retirement services division.
She first joined MassMutual in 1999 and held a number of key management roles in the company's retirement business, with experience in the annuity and trust businesses as well as management of the participant experience through the asset retention, call center and enrollment specialist functions. From 2005 to 2009, Desai led the Career Agency System (CAS) and TPA channels of the retirement business. Prior to joining MassMutual, Desai spent nine years with Fidelity Investments.
Linkedfa Appoints Eric Levine Executive Vice President of National Sales
Financial professional social networking site linkedFA announced September 21, that it has hired Eric Levine as executive vice president of national sales. He will be responsible for working with the financial industry to educate financial professionals about the business benefits of social media and technology solutions.
Previously, Levine spent 13 years at Investacorp Inc. as due diligence officer and director of business development.


text:
Spider-Dog Pet Costume
Martha's beloved French bulldogs are all dressed up for Halloween, too. Francesca, disguised as a devious black widow spider, is out to catch the little moth Sharkey in her web!
Source: Martha Stewart
The spider's bulbous body and angular legs are made of felt; once assembled, the costume is sewn onto a doggy-size T-shirt.
- Basic sewing supplies
- 36-inch square black felt
- Orange felt
- Fill
- Knitting needle
- Fabric glue
- Black dog T-shirt
Photocopy body, leg, and diamond templates cut out. Using the templates as guides, cut 2 body pieces and 16 leg pieces from black felt; cut 2 diamond pieces from orange felt. To make each leg, pin 2 leg pieces together and sew; using knitting needle, stuff tightly with fill. Sew body pieces together, leaving a small opening for fill. Stuff body, and sew opening closed. Glue the two diamonds to top body piece to make an hourglass shape.
Place each leg on body with the open ends sandwiching the body; hand-sew legs to body. Sew body to center of back of T-shirt. Tack along contour of body to stabilize.


text:
Thursday, May 6, 2010
SITF: Mud Cracks on Mud Cracks (Original and Next Generation)
The newer mud on the right is from late April, 2010 (almost active at the time of the photo); the older, sandier deposit on the left is known to be post-2008, probably from sometime earlier this year. The somewhat irregular and thin, crackly-looking younger mud cracks cover and sink into nicely formed, almost perfectly hexagonal mud cracks. The only area in the two photos where the more recent, still-wet mud shows sub-hexagonal shapes is in the upper right of this last photo.
Too bad I won't get to check out this muddy, crackly, alluvial area to see what develops next!
4 comments:
- Tony Edger said...
Mud cracks. I didn't have a clue. Once again, your blog prompted me to do some research, revealing the geological connections for this non-scientist. Probably old news to you, but there's an interesting paper on the web at http://www.lgoehring.com/Papers_files/SM2009_preprint.pdf which concludes that through repeated cycles of wetting & drying, the cracks in a clay layer evolve from creating rectilinear shapes toward hexagonal shapes with 120 degree angles. So cool.
- Friday, May 7, 2010 at 10:02:00 AM PDT
- Silver Fox said...
Very nice paper! Thanks for the link, Tony.
- Friday, May 7, 2010 at 11:05:00 AM PDT
- Tony Edger said...
Silver Fox: Thought you might enjoy the picture of salt flats in Bolivia that accompanied a travel article in yesterday's Washington Post. http://www.washingtonpost.com/wp-dyn/content/article/2010/05/06/AR2010050605640.html
- Monday, May 10, 2010 at 7:16:00 AM PDT
- Silver Fox said...
Nice! Salt-flat cracks.
- Monday, May 10, 2010 at 8:10:00 AM PDT


text:
stinkywigfiddle wrote:Alright then. Round 1 is now closed.
I'll be starting a new thread for the next round, I guess.
Umm. When?
I'm asking because we'd be glad to spread Juffo-Wup to a new world.
We would like to plant our child in the lot #3.
(am I'm violating some rules posting it here?)


text:
COLBERT, OK-Thursday, we told you about a veteran marine contractor who died while working for a company in Iraq. Family friends reached out to First News and told us they weren't getting straight answers about why his body couldn't be brought back to Colbert.
Tuesday, lawmakers and other state officials said they're doing what they can to help.
Michael Copeland was in Iraq, doing maintenance on aircraft for DynCorp. His father, Mike, said his son was on U.S. Embassy soil when he died. U.S. Congressman Dan Boren told us that hasn't been confirmed, but there were no signs of foul play. Mike said regardless where his son died or how, his body should be returned home and at least one state lawmaker agrees.
It's been over a week since Michael Copeland was found dead in his bunk in Iraq.
"There is absolutely no excuse in this world that you could give me that would convince me that there's a reason why he should not be home," said Copeland's wife, Angela.
Michael's father, Mike, said they were told by the State Department that Iraqi law requires an autopsy before a body is transported out of the country. Friday, they said they were given a different reason.
"We have learned that this rumor that Michael David possibly had SARS, which we are already pretty sure is not true. It's sure enough, not true," said his father, Mike.
"Michael did not have SARS. He would have been coughing, short of breath, difficulty breathing, fever, he didn't have any of those symptoms when I talked to him," said Copeland's mother, Connie.
The Copelands said they got a call from the State Department Tuesday morning and were told that the agency can't talk to them anymore because of Congressional inquiries and media involvement.
"I think they're hiding something," said Connie.
We left several messages with the State Department, our calls were never returned.
"In America, we have our laws, they have their laws there. He is an American citizen, he needs to be brought back home to American soil to his family so he can be laid to rest."
Oklahoma State Rep. Dustin Roberts said he's calling on state and federal lawmakers to help get Copeland's body back.
"Our congressional delegation from Oklahoma is working diligently to try to get Mr. Copeland back home and I applaud their efforts."
"What Gov. Fallin and her office has done is to reach out to the members of the Oklahoma congressional delegation and make it clear that if there's any way the governor can help to resolve the situation, our office is willing to provide assistance," said Alex Weintz of Oklahoma Gov. Mary Fallin's Office.
"He's been there for almost 12 days and whatever they're doing is not quite enough because he's still there," said Connie.
"I'm trying to stay optimistic and I'm thankful for any and all help," said Angela.
"We're gonna get him back home. We're gonna get him back home safe and sound so they can lay him to rest," said Rep. Roberts.
"My bags are packed since day one. I'll go over there myself if that would help, and bring him home," said Mike Copeland.
U.S. Sen. Jim Inhofe released a statement in response to Rep. Roberts' comments.
Inhofe said "it's insensitive and disturbing that a local state representative, like Dustin Roberts, would try to grand stand in the media over this heartbreaking loss."
He added that members of Congress are working with the State Department and are trying to resolve the issue.
Statement from U.S. Sen. Jim Inhofe:
WASHINGTON, D.C. - The office of U.S. Sen. Jim Inhofe released the following statement regarding the death of Michael Copeland in Iraq. Copeland is an Oklahoman who was working as a contractor in Iraq:
"Out of respect for the family who has lost their loved one in Iraq, we do not openly discuss our work on behalf of constituents. At the same time, it is insensitive and disturbing that a local state representative like Dustin Roberts would try to grand stand in the media over this heartbreaking loss. Had he bothered to pick up the phone and check with the congressional delegation, he would have learned that we have been working on this issue since last week when the family made us aware of it. We are working with the State Department to have his body returned to his family and the issue resolved as soon as possible." - Jared Young, Communications Director for U.S. Sen. Jim Inhofe (R-Okla.)
Statement from OK State Rep. Dustin Roberts:
Roberts Calls on State Leaders to Help Bring Oklahoma Citizen's Body Home from Iraq
OKLAHOMA CITY – State Rep. Dustin Roberts today is calling on Oklahoma Gov. Mary Fallin and the state's Senate and Congressional delegation to urge Iraqi leaders to release the body of former Marine and Colbert native Michael David Copeland, who died on June 9 in Baghdad while working as a private contractor.
According to Copeland's family, the Iraqi government has refused to release the body until an autopsy can be performed. The State Department told the family that Iraqi leaders believe Copeland died of Severe Acute Respiratory Syndrome (SARS), but the Copeland family says it cannot be possible.
"This is outrageous and offensive to the Copeland family," said Roberts, R-Durant. "It has now been 10 days and they still refuse to release his body so his family can give him a proper burial. Michael David Copeland was a man who served our nation as a Marine and our state as a National Guardsman, and his family deserves better than this. I am asking Gov. Fallin and our U.S. Congressman and Senators to look into this matter and help this family get answers."
Copeland's family has told Rep. Roberts that he was alert and spoke with his father on the phone just 12 hours before his death. The family said according to the Center for Disease Control (CDC) website, no person has died from SARS since 2003 and that Copeland displayed no symptoms of the disease.


text:
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text:
BEAVERTON, OR -- Several fitness equipment manufacturers as well as 24 Hour Fitness and Virgin Active Health Clubs are working with Nike Inc. and Apple Inc. to make Apple's iPod Nano compatible with cardio equipment, Nike and Apple announced yesterday.
The Nanos would track workouts, set goals and upload the information to a Nike Web site. Also, users can access other benefits, such as buying specialty songs or challenge other users to a workout goal by joining in social network components. The new technology is expected to be ready in more than 500 gyms worldwide this summer.
More than a year ago, Nike and Apple formed a partnership to create Nike+ technology in which the wireless system allows some Nike running shoes embedded with a sensor to communicate with Nanos. Apple and Nike both say shifting their attention to health clubs was the next natural step, and both companies say there are plans to grow in the future.


text:
"Top Ten Thoughts That Went Through Philip Humber's Mind During His Perfect Game."
10. Don't jinx it by thinking about it. Don't jinx it by thinking about it — I'm thinking about it.
9. Thank goodness for my catcher, A.J. Pierzanky… Piernoftski… Pierzonski… Whatever.
8. Go sit in the truck!
7. Thank goodness I drafted myself for my fantasy team.
6. The only thing better than pitching a perfect game is making a perfect meatloaf every time with my new Perfect Meatloaf Pan.
5. Can Broadway's "End of the Rainbow" really be like seeing Judy Garland in person?
4. Grab some pine, ducklips!
3. I see the Red Sox are up 9-0 on the Yanks — that's an easy win.
2. I wish I could get me one of them dancing horses.
1. Humber? How about Humbest!
The video is also on whitesox.com.


text:
Honestly, dear Berliners
What do you keep celebrating all the time?
Mondays. Tuesdays. Every day. The weekend. Him. Her. Tourism. Berlin has no industry anymore to speak of, so we live off the legend 24/7: freedom, tolerance, love, adventure. It's a piece of cake to sell and everyone wants to be a part of it, but it's pretty hard work.
What on earth are you wearing?
It's really hard to look like you don't care about how you look! That's why things get started later, people need time to get ready. High fashion has not caught on here despite Fashion Week, but many young designers from Berlin have gone on to conquer the world!
How many cities are there in Berlin?
Wait a sec, at least 96. That's how many districts we have in Berlin. There's Little Freiburg (Prenzlauer Berg), Little Istanbul (Wedding and Kreuzberg) and Little Cologne (the government district). More than 1.5 million people moved to Berlin after the Wall came down in 1989, we should be calling it New Berlin. There's no real division between East and West anymore because no matter which direction you go, sooner or later, you end up in the East.
Why are people here so rude?
What do you mean, rude? We call it being authentic. We have no time for false courtesies here. Live and let live is our motto, so go ahead and speak your mind as well, but how about a little smart repartee! There's no need to be so sensitive. Just because the bus driver made a joke at your expense doesn't mean he doesn't like you! He doesn't even know you! And at least you were noticed. In Berlin, not complaining about a person is praise enough.
Are curry sausages giving way to Hawaiian poke bowls?
Fat chance! The trendier the cuisine, the longer the lines outside the famous Curry 36 shacks. Caution: The main exhibits at the Curry Sausage Museum in Mitte district are tourists – they just don't know it.
Lufthansa operates a number of daily flights from Frankfurt (FRA) and Munich (MUC) airports to Berlin (TXL). Use the app to calculate your miles. Download here:
Lorenz Maroldt
The editor of Der Tagespiegel writes a daily newsletter called Checkpoint, which even non-Berliners enjoy.


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Welcome to the world of perfume bottle collecting
There is a fascination and intrigue to collecting perfume bottles. Whether a beginner or a seasoned collector, you will likely use these words (and more?) to describe our shared passion – Breathtaking! Captivating! Exciting! Challenging! Rewarding! Historically Educational! Good Investment! Beautiful Craftsmanship!
The pictures above are only a taste of the entire spectrum of this collecting specialty. Visit the Virtual Museum to see the various galleries that reflect the many aspects and sub-specialties of perfume bottle collecting. Perhaps you enjoy commercial bottles, or perfume burners? Novelties, Victorian laydowns, miniatures or art glass?
Did you think you were alone in the world enjoying this collecting specialty? Well, no longer. There is a large group of like-minded collectors who are passionate about this subject. They are the heart of this organization, producing a highly rated color quarterly magazine and presenting a world-class annual convention.
Want to know more? Request information with our online form. Want to know about the IPBA itself? Find out about IPBA. Have questions? Check out our FAQ.
We look forward to hearing from YOU and sharing in your passion for perfume bottle collecting.


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It's all about our customer...
Decisions are easy when you understand your values. At Benson Kearley IFG, serving you is our privilege. Our brokers are highly functioning, empathetic people who never compromise their pride or integrity in offering you the best insurance on the market with old-fashioned name-to-name service. Though our scope is global, our touch is local. READ MORE
The Community
Business is an essential, embedded part of this community, and we are conscious of that every day. We represent Benson Kearley IFG outside the office as well as in it, which is why each year we Participate in a number of community programs. It's about give and take.
Insurance Simplified
Insurance terms can be quite complex if not explained properly. Click out these helpful definitions that we've put together to make understanding insurance easy.
Newmarket Chamber of Commerce Insurance Program
Chamber of Commerce members work hard for our community. If you work for a member of the Newmarket Chamber of Commerce you may qualify for our preferred home and auto insurance rates.
Watch a message from our President...
We are independent insurance agency leaders at the top of our game, with our feet planted firmly in our community. Click on the icon below to watch a video from our President, Stephen Kearley.
Save when you combine!
Combine your home and auto insurance to get the best deal! Click here to start today!
Prefer to speak to someone over the phone? Contact us at 1.800.463.6503, Monday-Friday 8:30 a.m. to 5:00 p.m.
Report a Claim
Benson Kearley IFG is always here for you. To report a claim, please click here.
Prefer to speak to someone over the phone? Contact us at 1.800.463.6503, Monday-Friday 8:30 a.m. to 5:00 p.m.


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The morning after Clive Davis' Grammy party turned into a celebratory tribute, details surrounding the death of Whitney Houston began to emerge.
The singer was found unconscious and submerged in the bathtub of her room at the Beverly Hilton by her hairdresser Saturday afternoon, according to TMZ. She called security, paramedics were summoned and a bodyguard attempted to resuscitate her via CPR, but Houston was declared dead at 3:55 p.m.
Read more: Whitney Houston Dead at 48
An autopsy was performed Sunday but police weren't talking about the investigation and said there would be no updates until Monday. Results of the autopsy won't be known until toxicology tests are analyzed, which typically takes 4-6 weeks.
The singer's death, coming on the eve of Sunday night's Grammy Awards (CBS, 8 p.m.), threw organizers and those in town for the event into a state of shocked disarray.
Even as planners worked to incorporate a tribute to Houston into Sunday night's events, Recording Academy CEO Neil Portnow said "a light has been dimmed in our music community today, and we extend our deepest condolences to her family, friends, fans and all who have been touched by her beautiful voice."
Davis' Saturday night party, a must-attend for every star in the music industry, was set to honor Richard Branson. Instead on the red carpet music stars were asked about the shocking death of Whitney, 48, just hours earlier on the fourth floor of that same hotel.
Davis, who discovered Houston and became a mentor to her, was said to be "devastated." But in opening remarks at the party, he issued a call to celebrate her life.
"Whitney was a beautiful person and a talent beyond compare," Davis said. "She graced this stage with her regal presence and gave so many memorable performances here over the years. Simply put, Whitney would have wanted the music to go on and her family asked that we carry on."
Performances and tributes from artists such as Sean Combs, Tony Bennett, The Kinks, Wiz Khalifa and R&B singer Alicia Keys turned the mood into celebration of her life.
Bennett kicked off the evening with a subdued performance of "How Do You Keep The Music Playing," and gave a speech that recalled the tragic deaths of Michael Jackson and Amy Winehouse who, like Houston, had suffered from drug abuse during their lives.
"She always, she always hit you with that beautiful smile, she always hit you with that incredible energy, she gave you that hug, that grandma hug that just shook your body," Combs told the Daily Telegraph.
The 54th Grammy awards celebrate the best in American music, and the highlight of the show on Sunday was expected to be a performance by Adele, the first time she returns to performing since she had throat surgery.
Paul McCartney is also set to perform, and another anticipated moment is meant to be a reunion of the Beach Boys.
But Grammy organizers were scrambling on Saturday to incorporate the demise of Houston, one of the most successful pop singers of all time, and a winner of six Grammys.
In her career, Houston sold over 170 million albums and singles worldwide, with many hit singles. Her lead single, "I Will Always Love You," became the best-selling album by a female artist in music history.
She was the only artist to chart seven consecutive No. 1 Billboard Hot 100 hits: "Saving All My Love for You," "How Will I Know," "Greatest Love of All," "I Wanna Dance with Somebody (Who Loves Me)," "Didn't We Almost Have It All," "So Emotional" and "Where Do Broken Hearts Go".
A spokesman for the Grammy awards did not return calls about changes to the telecast, which airs on Sunday.
In what appears to have been her last public appearance, Houston attended the Kelly Price & Friends for the love of R& B event at Tru Hollywood on Thursday night.
She arrived with her daughter Bobbi Kristina and walked the red carpet. She took the stage to sing a brief duet of "Jesus Loves Me" before returning to her table. She left with her entourage after the performances.
Mikey Glazer contributed to this report.


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The Accessible Capitol Visitor Center
Although my disability from knee surgery is temporary, it has given me the opportunity to see Capitol Hill from a different perspective. Never have I appreciated the Capitol's consistently functioning escalators and elevators more than over the past month. From the elevators that take me from the Capitol Plaza to the Visitor Center's main entrance to the escalators that transport me to my office on the lower level, my journey has been trouble- and stair-free.
My colleagues and I at the Capitol Visitor Center (CVC) have made accessibility a priority with respect to all visitors with special needs. The tour of the Capitol is accessible, with elevators located close to the tour route. Brochures are available in alternative formats including large print, Braille, and HTML. Additionally, all restrooms at the Visitor Center are accessible, and family restrooms are available at all restroom locations. A public TTY is located near the North Gift Shop.
Visitors may request wheelchairs at the North Coat Check, located just to the right of the main entrance to the CVC. Shuttle service running from the southwest corner of Capitol Square to the CVC elevators on the plaza level, is available for people with mobility issues or who are using manual wheelchairs. Service animals are allowed in the CVC and in the Capitol.
Listening devices with an audio description of the CVC orientation film and Exhibition Hall are available, and all films at the CVC have open captioning. Sign-language interpreting for tours is available when booked in advance.
In the near future, the Office of Congressional Accessibility Services will release an audio tour of Exhibition Hall for people who are blind or have low vision.
My disability is temporary. But it's good to know that people with more permanent disabilities who are working at or visiting the Capitol are well taken care of.
Learn more about accessibility services at the U.S. Capitol Visitor Center.
See a map of accessible entrances to the buildings on Capitol Hill.


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Outrunning T. Rex
Q: Imagine a time-warp Kentucky Derby where somehow a 12,000-pound bi-pedal Tyrannosaurus rex chases a 1,000-pound modern racehorse. Could the thoroughbred run away? E. Arcaro
A: Horses are good middle-distance runners, with legs and body structure suited for speed and stamina, says Miami University interdisciplinary studies professor Nancy Nicholson. Probably the rocking fore and aft of a galloping horse helps pump air in (rock back) and out (rock forward), piston-like.
Computer modelings suggest T. rex was no slouch either, with amazingly complex motions during a stride, and feet possibly best for straight line runs or gentle curves.
Moment of suspension is a fascinating factor, where for a galloping horse all four feet come off the ground simultaneously– a moment of friction-free airglide. "It makes no sense to assign such a moment to a beast the size of an adult T. rex, because of the huge forces in falling even a few inches."
So give the horse a credit on this one, and debit the dino on its ecological role as cleanup crew on large kills by other species, which does not argue for blazing speed.
Put it all together, concludes Nicholson, and a racing horse may do 40 mph, the dino did a highly speculative 10-25 mph. "So, for a mile and a quarter on a curved track, I vote for the horse by a factor of 2, with terror likely inducing an inspired performance on the part of the horse. On a straight track for that distance, I would think the difference would be less, but still vote for the horse to win the Triple Crown."
Q: One thrill of parenting is knowing you're passing along your genes to future generations. OK, Moms and Dads, since 50 percent of your genes– on average– go forth in each child, how many kids do you need to have to assure passing on your full gene "slate," albeit in bits and pieces? M. Hubbard
A: Careful now. You can't just add 50 percent and 50 percent and assume two kids will do it. Problem here is your second kid's 50 percent of your genes overlaps half of your first kid's 50. So it's only 50 percent + 25 percent of you at this point, + 12.5 + 6 + 3 etc. for subsequent kids. So even after five kids there's still 3 percent of you "unexpressed," which just might be those beautiful eyes of yours or that gift for poetry.
How about 10 kids? Assuming 60,000 human genes, and other simplifications, by the time you get to your 10th kid you can expect to miss about 60 of your genes, on average, says Oxford University genetics statistician Carsten Wiuf. Make that 16 kids, and it's less than a single missed gene.
Still, that solo gene (or more) could slip through. To be rock-solid certain everything gets passed on, you'd need an infinity of kids. "In practice, 16 or so will do."
For a less exhausting approach, rely on your sibs and other family members to pass parts of you along.
Q: With only a handful of thumb tacks and a box of matches, devise a way to mount a short inch-thick candle on a bulletin board. Hint: Beware boxed-in thinking. J. B. Nimble
A: Solving this requires realizing that a box need not always serve as a container, says David G. Myers in "Psychology." Just empty the matchbox, tack it up on the board, and drip-stick the candle onto a horizontal surface.
Another classic: Try to connect nine dots in a 3-by-3 array, using four straight lines and no re-tracings or picking up your pencil. Can't do it until you see to go outside the "box," then it's easy.
Rather more fanciful flex-thinking let 60s celluloid hero James Bond, armed with only a ballpoint pen filled with poisonous ink, escape a small island surrounded by alligators, in the movie Live and Let Die, says Erick Lauber on his "Cognitive Psychology Tutor" Web site. "With death imminent, Bond notices that five alligators have lined themselves in a row stretching from the island to the mainland. Rather than try to outswim the beasts or kill them with his pen, he runs to the safety of the shore by stepping on the tops of the alligators' heads."
Some steppingstones! (DON'T TRY THIS!)
(Send STRANGE questions to brothers Bill and Rich at email@example.com)


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Quote:No comment about a Kung Fu style that has kata?Kata are japanese, Kung Fu is Chinese.
Quote:I was informed that Shaolin Kung Fu instead uses sets instead of kata so to speak and that you are kind of encouraged to make your own, though there are older ones of course. I think kata or sets should be only passed down master to student personaly unless you are going to make your own becuase they should hold the essence of the art.
Quote:Shuey,You have to admit that story line on the lineage of this style of Kung Fu seems an awfully lot like a bad MA movie. I mean if it's true....it's true. But I would certainly like to see some other disinterested info to confirm it.-B
Quote:As does mine coxne. We bring in many other grappling styles in class as well. Just because you have never heard of something doesn't mean that it's no good either.
Night club fight footage and street fights captured
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My daughter is going to Argentina and I am trying to make it as easy as possible for her to use a local sim. I did get her an Argentina sim card and she will have to activate it when she gets to Argentina. I want her to be able to use the Argentine sim for phone calls and switch to her Verizon sim for the Global data plan. Note Verizon charges $4.99 an minute for voice calls in Argentina.
The Question is does she have to set the options-mobile network to GSM/UMTS to use the local sim? If she puts the Verizon sim back in does she have to change the setting? Or should she leave it on Global?


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Sarah "Maria" BALL b. 1850 Compton Township, PQ was the daughter of Thomas BALL and Mary BRADY, both from Cavin County, Ireland. (and my great, grandmother).
According to a genealogy contact (Leslie Nutbrown) in Quebec a check of the 1815-1879 birth records in Quebec do not list a Maria BALL. There is a Sarah Ball born in 1850 in Compton, daughter of Thomas BALL and Mary BRADY. I subsequently found both of her names "Sarah Maria" listed on the birth record of one of her children.
She married William KERR 11 March 1873 in St. John's Anglican Church Waterville, Quebec and they had three children, Frederick James b. 1874, Bertha Eliza b. 1874 and Homer William b. 1881. Homer was my grandfather.
She died young at age 37 on 18 November 1887 leaving behind her husband and three young children, ages 13, 11 and 6.
Young Bertha was sent to live with her Boston BALL relatives and lost touch with the rest of the family. Family stories say she moved west to Idaho (?) after her marriage but this has not been confirmed.
I wish to learn more about the BALL family in Quebec, Ireland and Boston.
If anyone has a connection (no matter how remote) to this family please comment below.
[This post is part of the Smile for the Camera, 21st Edition Carnival by footnoteMaven at Shades of the Departed]


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For this giveaway I decided to go trawling the internet to try and find my favourite copy of the infamous Alexa! As I'm currently saving up for a proper Alexa and Im always asked about copies of the bag, I thought I'd give you all the chance to get your paws on a handbag yourself without having to save up a small fortune for a real one! I've been planning on doing a giveaway for this bag for what feels like forever, But I just kept holding out until I found THE bag!
The bag is a tan colour and made from PU Leather (ie. Not proper leather!). Its actually surprisingly nice quality for a non leather bag! Its about 35cm by 25cm and then 9.5cm width, Which is about the same size as the regular Alexa. Its got one large zipped pocket and a small pocket on the inside as well as a slot for a mobile phone (I always use the phone fab to put my lipsticks in!) As well as the twist lock, and it does have a long strap included too!
Anyway, Read the rules below to find out any information you might need to know! You dont need to have a blog, you just need to be a follower of my blog! The only extra entries are tweets or blogs about the giveaway! Please spread the word about the giveaway, Personally I think its absolutely fab and seeing as its one of the most coveted bags at the moment I'm sure you'd know someone who'd be interested in it!
- There will be ONE winner!
Tweet @llymlrs is giving away a fab Alexa-esque Bag! http://bit.ly/b59X2v
Post a link about this giveaway on your blog/sidebar!


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Amadou King (Feat. Dija Star) - Fight For Your Love [Prestigefilm Works Submitted]
To know more about Amadou please visit
Video Directed by Alex Acosta of Prestige Film Works 917-609-9708
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- Home Delivery
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2019 GMC Yukon
$69,654 • New
Asking Price
Ext. Color
Crimson Red Tintcoat
Stock Number
8 Cylinder
Int. Color
Cocoa / Shale
Four Wheel Drive
Installed Options
10 Speakers
3.23 Rear Axle Ratio
3rd row seats: split-bench
4-Wheel Antilock Disc Brakes
4-Wheel Disc & 4-Wheel VAC Power Antilock Brakes
4-Wheel Disc Brakes
5 Auxiliary 12-volt Power Outlets
ABS brakes
Adjustable pedals
Advanced Adaptive Cruise Control
Air Conditioning
Alloy wheels
All-Weather Floor Mats
AM/FM radio: SiriusXM
Auto High-beam Headlights
Auto-dimming door mirrors
Auto-dimming Rear-View mirror
Automatic temperature control
Black All-Weather Cargo Mat (LPO)
Black Assist Steps w/Chrome Strip
Blind spot sensor: Lane Change Alert with Side Blind Zone Alert warning
Bodyside moldings
Bose Active Noise Cancellation
Bose Centerpoint Surround Sound Audio System
Brake assist
Bumpers: body-color
CD player
Delay-off headlights
Denali Ultimate Package
Driver door bin
Driver vanity mirror
Dual front impact airbags
Dual front side impact airbags
Electronic Stability Control
Emergency communication system: OnStar and GMC connected services capable
Enhanced Driver Alert Package
Enhanced Security Package
Exterior Parking Camera Rear
Forward Automatic Braking
Forward Collision Alert
Front anti-roll bar
Front Bucket Seats
Front Center Armrest
Front dual zone A/C
Front fog lights
Front reading lights
Front wheel independent suspension
Full-Feature Reclining Bucket Seats
Fully automatic headlights
Garage door transmitter: HomeLink
Genuine wood console insert
Genuine wood dashboard insert
Genuine wood door panel insert
Hands Free Power Programmable Rear Liftgate
Heads-Up Display
Heated & Ventilated Driver & Front Passenger Seats
Heated door mirrors
Heated front seats
Heated Leather-Wrapped Steering Wheel
Heated rear seats
High-Intensity Discharge Headlights
Illuminated entry
Inside Rear-View Auto-Dimming Mirror
Integrated Trailer Brake Controller
IntelliBeam Automatic High Beam On/Off
Lane Change Alert w/Side Blind Zone Alert
Lane Keep Assist w/Lane Departure Warning
Leather Shift Knob
Low Speed Forward Automatic Braking
Low tire pressure warning
Magnetic Ride Control Suspension Package
Memory Package
Memory seat
Navigation System
Occupant sensing airbag
Open Road Package
Outside temperature display
Overhead airbag
Overhead console
Panic alarm
Passenger door bin
Passenger vanity mirror
Passive Entry System
Pedal memory
Perforated Leather-Appointed Seat Trim
Power door mirrors
Power driver seat
Power passenger seat
Power Release 2nd Row Bucket Seats
Power steering
Power Tilt & Telescopic Steering Column
Power Tilt-Sliding Sunroof w/Express-Open/Close
Power-Adjustable Pedals For Accelerator & Brake
Preferred Equipment Group 5SA
Premium audio system: IntelliLink
Push Button Keyless Start
Pwr Retractable Assist Steps w/Perimeter Lighting
Radio: AM/FM/SiriusXM w/Navigation
Rain sensing wipers
Rear air conditioning
Rear anti-roll bar
Rear Cross Traffic Alert
Rear reading lights
Rear Seat Blu-Ray/DVD Entertainment System
Rear window defroster
Rear window wiper
Remote keyless entry
Roof rack: rails only
Security system
SiriusXM NavTraffic
SiriusXM Radio
Speed control
Speed-sensing steering
Split folding rear seat
Steering wheel memory
Steering wheel mounted audio controls
Telescoping steering wheel
Theft-Deterrent Alarm System
Tilt steering wheel
Traction control
Turn signal indicator mirrors
Universal Home Remote
Variably intermittent wipers
Vehicle Inclination Sensor
Vehicle Interior Movement Sensor
Ventilated front seats
Wheels: 22/ x 8.5/ Bright Aluminum
Wireless Charging
Seller's Comments
$12,466 off MSRP! Priced below KBB Fair Purchase Price! Bradshaw Greer is pleased to offer this wonderful 2019 GMC Yukon Denali in Crimson Red and Cocoa/Shale Interior. Price includes: $3500 - Consumer Cash Program. Exp. 06/01/2020View More
14000 E Wade Hampton Blvd
Greer, South Carolina 29651
(888) 904-4584
Open Now 9:00 AM - 8:00 PM | DirectionsRead Dealership Reviews
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From Flab to Fab: Tone Up in 15 Minutes
Stop muffin top, nip hips, and trim thighs! This Pilates and plyometrics routine tones every trouble zone.
Karen Pearson
Targets: Abs, butt, hamstrings, inner and outer thighs
- Stand with feet wider than shoulder-width apart, toes turned outward 45 degrees and arms extended out to sides at shoulder level, palms down.
- Bend knees until thighs are parallel to floor and lower arms so that wrists cross in front of hips.
Karen Pearson
Bend and Stretch
Targets: Shoulders, back, abs, butt, thighs, and calves
- Start in downward dog: Get on all fours on mat, tuck toes under and press hips up to form an inverted V.
Karen Pearson
Targets: Arms, abs, obliques, butt, and thighs
- Lunge right leg out to right, toes pointing right, left leg straight. Press palms together overhead with elbows bent about 90 degrees out to sides (like a genie).
- Keeping hips squared, side bend at waist toward right.
- Next, reach with extended arms and torso toward right. Hold for 1 count, then pull torso back and bend elbows to return to side-bend start position.
- Do 10 reps. Switch sides (lunge and bend to left); repeat. Do 2 sets.
Karen Pearson
Kneeling Leg Circle
Targets: Shoulders, back, abs, butt, inner and outer thighs
- Kneel on mat, extend left leg out to left (toes on mat) and side bend at waist to right to place right palm on mat near right knee.
- Lift left foot off mat with toes slightly turned out; with leg, slowly draw 10 large clockwise circles, then 10 counter-clockwise circles.
- Switch sides, repeat. Do 2 sets.
Karen Pearson
Alternate V-Sit Roll
Targets: Abs, obliques, and lower back
- Sit on mat with knees bent, feet flat and arms by sides.
- With knees bent, lift feet off mat until shins are nearly parallel to floor and lean torso back 45 degrees into V-sit; extend arms forward.


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What do the Bayonne Bleeder and Dick Codey have in common?
We can't figure it out either, but Chuck Wepner, the guy who almost went the distance against Mohammed Ali in 1975 and inspired the movie "Rocky," joins the former governor in Codey's spoof video about his decision on whether he should run for the top seat in 2013. (Hat tip the The Record's John Reitmeyer for turning us onto the video).
"Alright," Codey opens. "I know I got to make a decision. But it's just not a decision to run against (Gov. Chris) Christie. It's also a primary race against (Newark Mayor Cory) Booker in June."
The former governor, who as Senate president took over and filled the rest of Gov. Jim McGreevey's term after he stepped down, gets in an early shot on Booker, too, comparing him to an oft-arrested and drug-addled starlet.
"Now here's a guy who gets more free publicity than Lindsay Lohan," Codey cracks.
Recently polling shows Codey and Booker as the two Democrats who would do the best against Christie in 2013. But the polls indicate that both would still lose to the Republican governor.
Codey, in the video, concedes it will be a tough slog, so he needs to get in shape. That's where Bayonne native Wepner comes in. To the theme from "Rocky," the two jog through the streets before Codey, wearing a hooded sweatshirt with the words "Governor Richard J. Codey" on it, collapses outside a government building.
We see two problems with the video's boxing analogies:
1) Wepner lost to Ali by technical knockout in the 15th round, an ominous precedent if Codey wants to be governor again. Codey talks of needing a different ending than the one in "Rocky" (he loses), but he didn't bring up Ali's TKO, which was probably a good idea, since Wepner still looks like he could pound someone into oblivion.
2) Codey never mentions his colleague, Sen. Barbara Buono, who has already declared her intention to run, instead focusing on Booker, who has not made up his mind yet. Could taking Buono lightly could lead to an early knockout? Buono's a runner, so she's got the wind to go the distance, and we suspect she has sneaky power in her left.
Hopefully, there will be another video installment to answer that question.
Here's the video:
ADDITION: We have to give Wepner his props. The guy really was a good fighter, and he's a New Jersey icon. Here's a video of Wepner's legendary 9th-round knockdown of Ali, who swears to this day that Wepner stepped on his foot, causing him to fall:


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Friday, May 22, 2015
RNA doesn't correlate with protein? Huh?
In our group, when we present work on RNA abundances, we are often faced with the question: "Well, what about the protein?" (fair enough). This is usually followed by the statement "Because of course it is well known that RNA doesn't correlate with protein." Umm, what?
I have to say that I'm a bit puzzled by this bit of apparently obvious and self-evident truth. I thought that most people accept that the central dogma of DNA to RNA to protein is a pretty solid fact in most cases. So… if you have more RNA, that should lead to more protein, right? Shouldn't that be the null hypothesis?
Apparently this notion has been around for a long time, though nowadays it is perhaps a bit more conceptually confusing due to a few recent results. Perhaps the biggest one was the Schwanhausser paper in which they compare RNA-seq to mass-spec and show that there is a distinct lack of correlation between mean RNA levels and mean protein levels across all genes (also the Weissman ribosome profiling paper). What this means, on the face of it, is that even if gene A produces more RNA than gene B, then it may be the case that there is more protein B than protein A. Fine. There are differences in protein translation rate and degradation rate, leading to these differences, no surprises there. Plus, Mark Biggins and Allan Drummond make the point that any measurement noise will lead to decorrelation even if things are very correlated, and their reanalyses seem to indicate that the correlation between RNA and protein may actually be considerably higher than initially reported.
The next example that's a bit closer to home for me is whether RNA levels and protein levels correlate, even for the same gene, across single cells. Here, it gets a bit more complex, and one might expect a variety of behaviors depending on the burstiness of transcription, degradation rate of the RNA and the degradation rate of the protein. Experimentally, there are some cases in which the RNA and protein of a particular gene do not correlate in single cells (Taniguchi et al. Science 2010 is a particularly good example). This may be due to long protein half-life, which effectively smooths over RNA fluctuations. In our PLOS 2006 paper (Fig. 7), we showed that there can be a strong correlation between RNA and protein when the protein degrades fast, and that correlation goes down a lot when the protein degrades more slowly.
And of course there's the whole world of post-translational modifications, like during the cell cycle, etc., in which protein activity and potentially levels change independent of transcript abundance. Well, dunno what to say about that, I'm biased to just think about RNA. :)
Nevertheless, overall, I think it's pretty safe to assume most of the time that if you increase RNA abundance for a particular gene, you will end up with more of the encoded protein. I think that should be the null hypothesis. If anyone knows of any counterexamples, please let me know.
Oh, and by the way, in case you're wondering, transcription also correlates with RNA.
13 comments:
- AnonymousMay 25, 2015 at 4:07 AM
What all those thousands of researchers who "over express" a protein using a strong promoter (e.g. CMV). I think that's a pretty good hint for the RNA-protein correlation...ReplyDeleteReplies
- AnonymousMay 25, 2015 at 4:18 AM
'What about..'Delete
About transcription - not neccessarily so - becuase there is this fine balance between transcription & decay. By looking solely at "steady-state" level, one cannot say anything about transcription or decay seperately. Because you can have increased transcription combined with rapid decay, or increased stability combined with reduced transcription, and in both cases you can end up with the same "steady-state" level.
Hi Gal, yep, I think the overexpression experiments are pretty solid evidence as well! You are of course right about transcription levels not being the whole story for transcript abundance. I guess what I meant is that if you increase transcription of a given gene, you will end up with more transcript of that gene. The relationship between genes is certainly confounded by half-lives, etc.Delete
Hi Arjun,ReplyDelete
If we only increase the levels for a set of mRNAs without other changes, the corresponding protein levels must go up. The only exception is if the ribosomes are completely saturated by those transcripts, which is very unlikely.
However, the picture is different if we study two conditions that differ substantially, i.e., two different cell types. They have different RNA-binding proteins, many mRNA have different/alternative UTRs with known regulatory roles in translational regulation, and protein degradation times are different. The latter is one of the most interesting results from the Jovanovic paper that Mark Biggins was asked to highlight. In this context, predicting that protein levels are linearly dependent on mRNA levels is analogous to predicting that mRNA levels are linearly dependent on DNA levels. In fact, mRNAs are linearly proportional to DNA levels in the case of gene duplications but of course this does not prove the more general point. A general example of translational regulation is early development of lower metazoans; lower metazoans generally shut-off transcription and use translation to make protein from the existing mRNAs in a highly developmentally regulated fashion.Replies
Hi Nikolai,Delete
You are of course right: context matters. The thing that bugs me is just the blanket statement that protein doesn't correlate with RNA, often in cases where it makes no sense. I think that the Jovanovic paper also shows that most proteins correlate with RNA. I think that's a fairly reasonable null hypothesis. I think the question of whether one *needs* to check that the protein indeed follows the RNA in any specific case is somewhat tricky and context dependent.
I think it's also interesting to think about some bounds on translational regulation. To me, I think there can only be so much regulation at the level of translation, reason being that there's simply much less sequence space there to specify regulation. In higher metazoans, gene regulation requires a lot more bases to encode than is available in the UTRs of most RNA. Thus, I think it's pretty reasonable to assume that *in general* there is more regulation at the level of transcription, although I have not really done any analysis of this myself. Of course, there will be a lot of counterexamples.
Those experiments in early development are really cool! I know in C. elegans you can shut off zygotic transcription and the thing still develops fairly normally to the 100 cell stage. Of course, some fraction of that is protein localization rather than translation. Any idea what percentage?
Eric Lecuyer et al. estimated that in Drosophila over 70 % of the mRNAs are localized and thus their translation will result in at least partially localized protein. All of the well studied localized mRNAs (e.g., bicoid, oskar and others) are also translationally regulated and this translational regulation is essential. The best global estimates that come to mind about translational regulation in early development are from Xenopus. They are all whole embryo estimates, thus independent of localization, and report very weak or no correlation between most mRNAs and proteins, with a few prominent exceptions. I have examined some of these data, and I am convinced that in this context the lack of correlation is not due to noise.Delete
I fully agree with you that these examples should not be interpreted as the general rule and certainly do not warrant requests to "check that the protein indeed follows the RNA". This latter request is frequently unwarranted, and I think even somewhat separate from the discussion on what is the level at which a particular gene is regulated. The fact that bicoid is translationally regulated does NOT mean that its mRNA level is not important and should not be studied.
- AnonymousMay 29, 2015 at 9:07 AM
Hi Arjun,ReplyDelete
In my discussions with immunologists, statements like "mRNA and protein doesn't correlate" often come up. The one example that is often given is that of NF-kappB, whose activity is induced after LPS stimulation by post-translational modifications that are independent of new transcription. The general consensus among immunologist is therefore that NF-kappaB's activity increases in the absence of an increase in its mRNA.
But, if you check the mRNA levels after stimulation of the genes encoding the subunits of NF-kappaB, they tend to go up too. So, even in this example case of "lack of correlation" between protein (activity) and mRNAs, in the end there is a clear (time-lagged) correlation. It's a funny case when you think about it: people often (correctly) claim that "correlation does not imply causality", but in this case, they do seem to have accepted that "lack of causality implies lack of correlation". This latter statement is just as incorrect as the former one.
I have similar feelings about the notion that "alternative transcripts are not correlated".
- AnonymousDecember 6, 2016 at 8:39 PM
I was wondering if you have come across examples where you can reliably detect a protein but not mRNA transcript of the same gene? What sort of things would explain this? thanks.
I don't *think* we've encountered such an example. We've definitely found cases where neither RNA nor protein are reliably detected, much to the chagrin of collaborators… :)Delete
- AnonymousDecember 7, 2016 at 11:34 AM
ok thank you.Delete
This question troubles protein biochemists as well. I've spent many nights reading the literature to check the evidence - summarized in this white paper http://www.kendricklabs.com/WP1_mRNAvsProtein-New2014.pdf. At best there's a 40% correlation between protein and mRNA levels. The correlation is highest for housekeeping proteins and poorest. ~25%, for regulatory proteins like tyrosine kinases.
Metazoan evolution has had 700 million years to mess with cell growth mechanisms. When you think about the protein synchronicity required for generation of the numerous cell types that make up any mammalian organism, the disparity is not surprising. Post-translational modifications are running the show.
That's my 2 cents anyway.
in response to perturbation, the mRNAs that change in abundance do not translate fast enough to be important at the protein level to survive the perturbation. evidence, most of the genes that transcribe these mRNAs can be deleted with out effect.ReplyDelete


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Steve Watson
Prison Planet.com
April 19, 2013
The father of the two suspects in the Boston bombing, Tamerlan and Dzhokhar Tsarnaev, has told Russian and Western media that he believes his sons have been framed by US intelligence.
A report from the Russian agency Interfax [1] says that a correspondent spoke with Anzor Tsarnaev, who resides in the southern Russian republic of Dagestan.
From the Interfax report:
"I learned about the incident from TV. My opinion is: the special services have framed my children, because they are practicing Muslims. Why did they kill Tamerlan? He was supposed to be caught alive. The younger is on the run now. He was a sophomore at a medical school in the U.S. We expected him to come home for vacation. Now I don't know what's going to happen. Tell you once again: I believe special services have framed my children."
Reports on Twitter also claim that Anzor has said that "all hell will break loose [2]," if his remaining son, who is currently on the run in a suburb of Boston, is killed.
The AP reports [3] that the father of the suspects also spoke with their own reporters. During a short conversation Anzor Tsarnaev was said to have told the reporters that his children were 'true angels'. He also said that he has two more daughters who live in New York.
He described his younger son as "an intelligent boy" who was studying medicine, and said that both his children are innocent.
"They were set up, they were set up!" he exclaimed. "I saw it on television; they killed my older son Tamerlan."
The father is then said to have become more agitated and abruptly ended the call angrily, saying, "Leave me alone, my son's been killed."