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About deane
because at this point me and countless others are fighting a losing battle against BLATANT censorship. their is no difference at this point as to whether all these court rulings make me and countless others victims of mine and others free speech. the RIAA and the MPAA have blatantly made the laws that SILENCES ME.
hmm manufacturing a terrorist plot = those responsible need to be brought to justice since they have committed a crime (or at least are complicit for creating a crime)
thanks DOJ/MPAA/RIAA and all the others. you have by your actions CENSORED ME! the government has now BY LAW decided my political speech is to be silenced. >.
yet another company to boycott. what a shame capcom. you lost me as a customer. though I never played your games anyways! good riddance, see you in bankruptcy some day :-)
personally I smell a massive lawsuit Against I.C.E for kidnapping/illegal detainment/and whatever else a responsible attorney can think up. maybe now I.C.E will learn to do their primary job so much better ;-)
and by "they made" I mean original work. not just a remix but something that BY LAW is copy protected. even my comments FYI is an example of that copy protected content. if the government seized this website for instance (doubt it would happen, just a hypothetical) all the comments we have made would then amount to them censoring us. think of that people. THAT IS WHY we are all up in arms here!
and by "they made" I mean original work. not just a remix but something that BY LAW is copy protected. even my comments FYI is an example of that copy protected content. if the government seized this website for instance (doubt it would happen, just a hypothetical) all the comments we have made would then amount to them censoring us. think of that people. THAT IS WHY we are all up in arms here!
if you wish to support SOPA/PIPA/protect IP then, you are supporting Censorship. end of story.
if you support the seizure of web domains with out due process of law you support Censorship. the reason for this is simple. anybody could and I bet Has published something they made of interest to the readership of a website. when the government seizes and takes that web domain down, they have censored that persons free speech!
lets lay out some things first of all.
if E-PARASITE 1 thing that will cease to exist is anything GNU/LINUX Related since US CoC says its infringing already!
2nd thing that will cease to exist is any form of protest on youtube/google/any other site that allows comments.
3rd thing that will happen is that the real pirates, namely ALL the malware authors will start eroding what little security we have any more on the internet.
sounds fun right? where will I and many others be able to make MY VOICE, MY VIDEOS, MY COMMENTS heard? this is the true scenario that mike and others are talking about! and yes it will make "pirating" possibly disappear however it will come at a cost that would make all of us sit up and cry NO!
if it worked as promised maybe it might be ok. thing is though this whole debacle is a classic bait and switch! also let me ask you this, why is DRM around when under the law it takes the rights of so many people away? case in point would be me wanting to make a commentary/voice over of the movie. CAN'T do that since well you can't change it with out breaking the law! DRM IS DEFECTIVE BY DESIGN!
the problem I see is sony would even take something like experiablog.net. any name the blog took would get the nasty grams. they now are in a no win scenario and never mind WHAT will sony now do with the site? NOTHING!
>The party is over Freetards! Soon all you pirates will be >going to prison and will lose your internets, just like the >Complex.com pirates are!
now to find a lawyer to prosecute you for libel!
interesting and in before the trolls. if you create something new the RIAA/MPAA will take you down even though what they are doing amounts to libel. hope somebody like this will sue them for libel/defamation ;-)
well good that GEMA is in for another smack down. GEMA is now the, and I use the term loosely; "PIRATE". if on one hand you wish to use the following terms to describe all the parties that do digital infringement, namely "freetards", Pirates, thieves, theft, or others; then when a person puts out something and the collective "industry" decides to do the same then THEY MUST be called the same things! GEMA saying they OWN something that they don't is committing all the things they are accusing others of! >.
simply put on police brutality, they should be tried as traitors since they swore to protect the people and defend the constitution and have knowingly broken that vow!
well I wonder when the lawsuits for false imprisonment and police brutality/harassment/and whatever else the lawyers guild might think up will happen :-). my personal opinion is that the police/government will lose out on this 1.
Dear AC,
mike is pointing out the direct cost of patent trolls sucking money from companies/individuals that actually create. if you wish think of it this way simply. for each dollar made 50 cents is then taxed by the trolls. where that money goes IS NOT AN ISSUE! all the actual inventors/creators are losing out and having NO desire to then make things causing less innovation.
to all the detractors,
let us say for the moment that one of the admins of a supposed "INFRINGING" site decides he will put a video denouncing all the profits and tax abuse that the RIAA/MPAA does. he posts this video on his site, and tells the sites users to spread this far and wide. his site is pulled by ICE and his video taken down. has a 1st amendment violation happened? this is just 1 possible way for his civil rights to be taken away. another is a user of the site does the same thing. its all the same under the law. censorship in ANY capacity is wrong. no matter whether a site WHICH HAS never stood a day in court to even be tried for ANYTHING has been censored.
finally a good part against WB/RIAA/MPAA! the agreement that WB makes when they use the provided tool constitutes a contract which the court should uphold. and then maybe just maybe WB is kicked out from lawsuit when they are sued by all affected parties!
"So what the judge is saying is that any lawyer in any civil case who offers a "settlement before going to court" is somehow a money grubber?"
actually I believe its more the fact that the court should not be a "Collection Agency" for the lawyers and others. ask yourself this, What is the purpose of the court?
"I would say that the judge needs to look at the other side, and see that massive copyright infringement is taking away millions of dollars from the useful arts.(citation needed, WHERE is this loss of million of dollars coming from; the public sure would LOVE to know) Perhaps a little balance might clear up the judge's clearly clouded vision."
and by balance what do you mean? the fact that he should proceed to just give the IP address info? or the fact that joining together separate parties and creating a class action lawsuit would be best? the court is in the business of justice and that usually means giving the parties a chance to actually get justice. if the court believes each individual should be tried separately then others that's fine by me since actual justice happens.


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Cook pasta according to directions until al dente. While the pasta is cooking place olive oil, red pepper flakes and onion in a large skillet. Sauté on medium until golden. Add garlic, artichokes and yellow pepper. Sauté until golden. Add broth, olives, garbanzo, tomatoes and salt. Simmer 5 minutes. Place drained pasta in a large bowl, pour sauce over and toss. Season to taste with pepper. Garnish with parsley and serve.
Per serving - 571 calories, 12 g fat (19% calories from fat), 22 g protein, 94 g carbohydrate, 11 g fiber, 0 mg cholesterol, 294 mg sodium

subset: commoncrawl


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Angry Scallops with Citrus-Spinach Salad
By á-7246
- Flour Mixture:
- 1 cup all-purpose flour
- 1 1/2 tablespoons California chili powder
- 1 tablespoon Gray Salt
- 1 teaspoon freshly ground pepper
- Salad:
- 2 blood oranges, peeled and sectioned, pith removed
- 2 tangelos, peeled and sectioned, pith removed
- 2 grapefruit, peeled and sectioned, pith removed
- Salt and pepper
- Extra-virgin olive oil
- 2 pounds (16 to 20 count shrimp) 4 per person, peeled but with the tail on
- 6 large cloves garlic, thinly sliced
- 2 serrano or jalapeno peppers, thinly sliced
- 1 1/2 cups basil leaves, stemmed and cleaned
- 1 tablespoon orange, julienned
- 1 bunch basil, cleaned
- 3 cups pre-washed spinach, stems removed
Step 1
Prepare the flour mixture for shrimp:
Combine the ingredients and set aside in container. This step can be done the day before dinner.
Peel and cut the citrus fruit into sections over a plate. Drain the juice from the plate into a bowl. Season liberally with gray salt and black pepper. Whisk in olive oil to form a vinaigrette (it should be in proportion, about 2 parts juice to 1 part olive oil).
Season citrus sections with salt and pepper. Drizzle a little of the vinaigrette on them to marinate and put them on a platter.
Have your fishmonger peel the prawns but leave the tails on for handles. Season liberally with salt and pepper. Preheat a sauté pan on high. Dredge the shrimp in the flour mixture. This will give them a nice crust and allow them to stay crispy and fresh at room temperature. Add olive oil to the pan and put in 1 layer of shrimp. You want them to caramelize, so don't toss or move them. Add a little more oil if the flour absorbs what's in the pan. After about 3 minutes, turn over the prawns. Let it caramelize, toss and turn out onto a cookie sheet. Add about 1/4 cup more olive oil to the already hot sauté pan. Drain pan juices from the cookie sheet into the sauté pan. Add sliced garlic and let it get light brown. Add chiles and let them get soft. Add the basil and stand back, there's a lot of water in basil and it will pop. Let it get crispy, less than a minute. While this is happening, arrange prawns over the citrus salad. Add the orange zest to the basil/garlic/chile mixture in the sauté pan. Season with salt and pepper, then spoon basil/garlic/chile mixture over the prawns.
Toss the spinach with the citrus vinaigrette and mound on top of the prawns. Serve.
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This is a formal invitation for all you awesome people to join us at the Forest this week for amazing performances, workshops and art:
Monday 19th July
9pm Film Night
'Fast food nation'
Tuesday 20th July
7-8.30pm Gudrun Hirt
Beautiful songs in different languages, voice improvs over magical instrument
9pm-close Fundraiser for Jakarta social centre
The specific Heats and The hermit crabs
Wednesday 21st July
7-8pm The Gramophone Hour
78s from 7 to 8
9pm-close Golden Hour
Best poetry and music around!
Thursday 22nd July
7-close Botanical Grove
Theatre workshop in prep for the Fronge Gallery at Botanics. Open to all!
9pm-close Avital Raz
Folk-Alternative Fusion from Jerusalem
Friday 23rd July
7pm-close Different Drummer
Sounds from all over the world plus special guests
Saturday 24th July
7-close Plastick Fork
Sunday 25th July
9-close Flution
Jillian Mathews & Grainne Rooney
present a funky fusion of flute and harp
All workshops are FREE and open to everybody.
Share skills,
create a workshop.
Contact: firstname.lastname@example.com
Mon, 11-12am: Chi Kung lesson (Hall)Mon, 12-1pm: Self Defence for beginners (Hall)
Tue, 11am: Healing meditation
Tue, 6-7pm: Nacho Picchu, Spanish (Action Room)
Tue, 7-9.30pm: Forest Choir (Hall)
Wed, 2pm: Gaelic workshop (Cafe)Thu, 11-12am: Chi Kung lesson (Hall)
Fri, 11am: Healing meditation
Fri, 5-6pm: Self Defence for beginners (Hall)
Sat, 5-6pm: Nacho Picchu, Spanish (Action Room)
Sun, 3pm: Letter Writing (Front Room in Cafe)
Sun, 7.30pm: Forest Drama Group (Hall)
12th-25th July Forest is 10
Celebrating 10 years of Forest hitory with photos,
stories and trees.
Old Hat books
Free Radical Book & Hat Library
Mondays - Wednesdays – Saturdays: 4pm – 8pm
The weekly programme 19-25 July
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'it's not a lie if you believe it' - George Costanza
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More than a feeling: A desire for God
You're longing for God--you just don't know it, yet.
A few years ago I was having lunch with a friend who is an agnostic. I had just finished my annual eight-day retreat at my favorite place to pray, Eastern Point Retreat House in Gloucester, Massachusetts, right on the Atlantic Ocean. Despite her agnosticism, she was interested in hearing about my retreat. So I told her a little about my prayer.
After a few minutes she said, "Boy, it must be nice to have had an experience of God. There's nothing I want more."
She's not the only one. The most frequently mentioned barrier to a personal relationship with God is never having experienced God's presence. "If only I felt something," some Catholics have told me, "then it would be easier to pray." "If only God made his presence known," say seekers, "then I could start down the path of faith." Even agnostics like my friend-as well as atheists-seeking intellectual proofs for God's existence admit that if they saw a glimmer of God, maybe they'd consider believing.
But people regularly have spiritual experiences. How do I know this? They tell me. The problem is that they don't take them seriously. "Oh, I was just being emotional," they'll say afterwards. Just as often, they ignore them. Or they reject them. Or they're simply not encouraged to talk about them.
Let's take one of the most common spiritual experiences: the desire for God. "O Lord," wrote St. Augustine in the fourth century, "our hearts are restless until they rest in you." Desire is a key way that God's voice is heard in life. And ultimately, our deepest desire, planted deep within us, is our longing for God. In short, the desire for God comes from God. It's a primary way that God draws people to the divine.
Still not convinced? Here are the most common ways that our holy desires reveal themselves in daily life. Maybe one will sound familiar.
Incompletion. "Is that all there is?" sang Peggy Lee in the 1960s. "I still haven't found what I'm looking for," sang Bono in the 1980s. At times you may have had the feeling that, even though you've had some success and happiness, something's missing. Part of this nagging restlessness never goes away and never will go away; in fact, it provides you with a glimpse of your longing for God. This is what one writer called the "God-shaped hole" that only God can fill.
Henri Nouwen, the Dutch spiritual writer, once wrote, "Somewhere deep in our hearts we already know that success, fame, influence, power, and money do not give us the inner joy and peace we crave. Somewhere we can even sense a certain envy of those who have shed all false ambitions and found a deeper fulfillment in their relationship with God."
What you're experiencing in those moments is your innate longing for God-planted in you by God.
Common longings. Sometimes you experience a strange or fleeting desire in the midst of an everyday situation: standing silently in the snowy woods on a winter's day; staring into the wide eyes of a tiny baby; finding yourself moved to tears during a movie. You feel an inexpressible longing to savor this feeling and understand what it is. Such common longings are ways of becoming conscious of the desire for God.
One friend, a self-described workaholic, described being "overtaken" by strange feelings during a baptism. She began to cry and said that she didn't know where this "feeling of peace" was coming from. She dismissed it as being overemotional. For me, however, it seemed clear that it was something else: a longing for what St. John of the Cross called "I know not what." That is, God.
Uncommon longings. Sometimes you feel a powerful, unmistakable, almost mystical, sense of longing for God, or of having a fleeting connection to God, triggered by unexpected circumstances. Mysticism, despite what you may have heard, is not simply the province of the saints: Karl Rahner, the German Jesuit, often spoke of "everyday mysticism." These are more intense feelings than common longings. One young man described the experience as if he were a crystal vase with God's love like water about to overflow the top.
Again, these feelings may happen more often-and to more people-than you may think. "For what is the mystical life," writes Ruth Burrows in Guidelines for Mystical Prayer (Continuum), "but God coming to do what we cannot do; God touching the depths of our being?"
Exaltation. Here you feel lifted up, or likewise, a sense of exaltation or happiness. Different from longing to know what it's all about, you feel that you are very close to, or are about to meet, the object of your desire. You may even feel the warmth of being near God, though you may be reluctant or embarrassed to define it as such.
Each of these feelings, by the way, may overlap. And there's no need to identify them precisely. Sometimes the feelings that are the most difficult to describe are those that are the most personal, the most tailored to your own situation. Here God may be speaking more clearly than anywhere else. Just because you can't describe it doesn't mean it's not real.
Clarity. There is an old New Yorker cartoon that features a wizened, monkish-looking man hunched over a large book. "By God," he says, "for a minute there it all made sense." Sometimes you feel tantalizingly close to understanding exactly what the world is about. Yes, you think to yourself, now I get it. On the morning of my ordination to the priesthood, at a church in Chestnut Hill, Massachusetts, I thought, "This is where I should be."
In her book Out of Africa, the Danish writer Isak Dinesen writes of being taken up in an airplane for the first time and peering down at the Kenyan landscape. "I see," she wrote, "This was the idea. And now I understand everything." (Meryl Streep repeats these lines in the film as Robert Redford pilots the plane, the music swelling on the soundtrack.)
Desires to follow. These desires are much more explicit. It is not a desire for "I know not what," but for "I know exactly what." You may be reading something about Jesus or another religious figure like St. Francis of Assisi or Dorothy Day or Pope John Paul II, and say to yourself, "That's the path I want to take." This is another way that God calls to us. You may be able to identify that as the desire for God.
Desires for holiness. An attraction to personal holiness is another sign of the desire for God. This can be triggered in at least two ways: first, learning about holy people in the past; and second, meeting holy people today. Holiness is naturally attractive, since it is an invitation into the divine. "Deep calls to deep," as Psalm 42 says. Think of the crowds that follow the Dalai Lama or Mother Teresa. But holiness is not reserved for the saints, who were human after all. Holiness always makes its home in humanity.
Vulnerability. Here's an often misunderstood statement: Many people feel drawn to God in times of suffering. Often people scoff at this response, saying it's just a crutch. Or it's a manifestation of superstition. But we do not turn to God in suffering because we suddenly become irrational. Rather, God is able to reach us because our defenses are lowered. You are not less rational. You are more open. Vulnerability can awaken your innate desires for God, which have been buried under layers of resistance.
Anyone, at any time, in any of these ways, can become aware of the innate desire for God. Moreover, finding God and being found by God are linked, since those phenomena have God as their source and goal. The beginning of the path to God is trusting not only that those desires are placed within us by God, but that God seeks us in the same way we seek God.
And that's what I told my agnostic friend over lunch. Her desire to experience God comes from God. She's still thinking about that one, she told me recently.
So the next time you're tempted to think that you've never experienced any direct experiences of God, or have never had a "spiritual experience," think again. Or, more politely, consider those experiences of desire, which are one of the primary ways that you can hear the echoes of God's voice in your life.
This article appeared in the July 2010 issue of U.S. Catholic (Vol. 75, No. 7, pages 37-38). It was adapted from a selection from The Jesuit Guide to (Almost) Everything (HarperOne, 2010).
Image: Andrew Gill


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Many of us have one - an ancestor who was a stinker, put quite frankly. And this can be quite a can of worms when writing and documenting our family histories.
I descend from William Nickerson, a fellow who gave the colonial government quite a run for their money back in the 1600s, and was well-documented for it. There are amply written, unbiased sources documenting his behaviors and punishments, and it's a part of who he was. He poses no problem for me in writing the family history - he was a character, and his own person, and no one is likely to be offended by what I write about him.
Then there's Aunt L. She's not so far back in history, having departed this life not quite 30 years ago. Many in the family still remember her. She left no descendants that might be more easily offended than the rest of us. But still, how exactly do I handle her in the family history?
She was my grandfather's aunt, and out of his own mouth come the memories of her locking he and is brothers in a dark closet and terrorizing them, and calling them names, because she hated their father. Or all of the Christmases that the girls got gifts and the boys got nothing. One of her nieces has less than fond memories of her as well, saying that she tried to cheat their mother out of anything that she could, be it family heirlooms, inheritance, or their brother's insurance money.
My own memories are much tamer, but then, Auntie was quite a bit older by the time I knew her. Once a year, at Christmas, we would gather at her house for a Christmas dessert and open small gifts. She got out the family china, and spent time trying to tell us about her father and mother, and trying to show a largely (unfortunately) uninterested bunch of people about the family history. No one, including me, seemed to care at the time. Under that tame exterior, though, still lurked the same anger and temper that she had as a young woman.
After a bad fall, she ended up having to go into a nursing home. She was furious. And it was my grandfather, the same little boy she terrorized as a child, who looked after her. He and my grandmother went to her house those last few years she lived there, and mowed the lawn, took her shopping, helped her clean, and visited with her... and in the nursing home, they went out twice a week to see her. One particular week, they took me with them. I was standing in the doorway when Auntie L., in a fit of rage, suddenly kicked her trash can violently out into the hallway. Two older gentlemen with walkers were approaching when the projectile shot out of her room, ricocheted on the opposite wall and came to rest in the middle of the hall. Without missing a beat, one of them said, "Well, looks like another one kicked the bucket!" Nursing home humor... not a great situation, but it has ended up being one of my favorite memories of Aunt L. It was so very... her.
So, do we try to leave future generations with positive impressions of their departed family members, or do we do our best to capture them as they were, warts and all? Should the wishes of other family members be taken into consideration, and if so, to what extent? Do we, as family historians, respect truth, or respect the dead? Is there a way to to both?
Thursday, October 31, 2013
2 comments:
If they have been gone a long time I would post it like it was but if recently deceased no. You will probably will be writing what everyone else was thinking.ReplyDelete
I vote warts and all. However, if the deceased still has living children. I'd probably keep the warts private for now. I never met my grandfather, but I have yet to hear one good thing about him. True, he had a rough childhood, but so have others who have managed to rise above it. So one day, I'll write about my grandfather so my children and grandchildren will know he was human, just like them.ReplyDelete
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text: Legal Protections for Workers with Different Immigration Statuses Michael Litrownik, O&G Employment Law Blog, January 9, 2020 Continue reading Amicus Brief: Lawyers for Civil Rights (LCR) and the law firm of Outten & Golden LLP Co-authored by Ossai Miazad, Michael N. Litrownik, Chauniqua D. Young, Rachel Williams Dempsey, et al., October 4, 2019 PDF file (555.74 KB) Recent Trends in Executive Compensation in the United States Wayne N. Outten and Cody Yorke, Outten & Golden LLP PDF file (137.74 KB) Continue reading Can Lenders Legally Discriminate and Limit Access to Credit Based on Applicants' Immigration Status? Michael N. Litrownik, O&G Employment Law Blog, August 15, 2018 Continue reading Moving Backwards: Trans Military Service Michael N. Litrownik, co-written with Toby Rae Irving, O&G Employment Law Blog, July 28, 2017 Continue reading Walmart's Failures to Support Workers Michael N. Litrownik, O&G Employment Law Blog, June 20, 2017 Continue reading Activists Challenge Wells Fargo on Many Fronts at Annual Shareholders Meeting Elizabeth Stork, O&G Employment Law Blog, April 27, 2017 Continue reading Article Co-Authored by Wendi Lazar Reveals Why Women Lawyers in BigLaw Earn Less Than Male Colleagues and Ways to Close the Pay Gap Wayne N. Outten, O&G Employment Law Blog, December 28, 2016 Continue reading Working Families in America Are Forced to Wait for a Raise Elizabeth Stork, O&G Employment Law Blog, December 2, 2016 Continue reading Trump to Sexual Harassment Victim, "You're Fired!" Co-authored by Kathleen Peratis and Nina Frank, O&G Employment Law Blog, August 9, 2016 Continue reading Pagination Current page 1 Page 2 Page 3 Page 4 Page 5 … Next page → Displaying 1 through 10, out of 77


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Finserve Marketing necesitaba un nuevo diseño de página web y lanzó un concurso de diseño en 99designs.
Un ganador ha sido elegido entre 6 diseños de 4 diseñadores freelance.
The website "forextradingonline.at" will be focused on reviewing and providing information on online forex trading websites.
Online forex trading is where people can "trade" one currency against another – e.g. bet that that the pound will rise against the dollar. This is not to be confused with foreign exchange websites where people buy currency for their holiday.
Forex trading may be considered a niche or product within the wider market of financial spreadbetting where people bet on whether a share (e.g. Vodafone), commodity (e.g. Gold) or currency (US$ vs. Euro) will go up on down.
Some examples of online forex trading websites are:
• http://www.Etoro.com
• http://www.Forexyard.com
• http://www.GftForex.com
There are also more general financial trading and financial spreadbetting companies such as http://www.igindex.com, http://www.cityindex.co.uk and http://www.cmcmarket.com.
Please note these examples are only to give you an idea of the sort of forex providers we will be providing information and reviews on – they're not necessarily examples of designs we like, or websites that have been put together well.
Most importantly this is your design - We do not wish to push you in any particular direction as to the style or design of the site as we believe you are the talent and wish to allow you to let your talent shine through.
Promote online forex trading and utilise the "theme" of the domain name in unison. And Although the domain is .at (Austria) it is targeted specifically at English language speaking countries with a strong bias towards the UK audience.
The theme may be that you can trade forex online ".at" home, in the office – anywhere – being online makes it easy. But as I say the creative freedom is yours.
The visitors to the website will be those that have an interest in trading forex online. They are likely to be male, aged 25-40 and either have an interest in more traditional share trading but like the simplicity of the forex sites, or they have a background in enjoy gambling online (poker or casino) and like the thrill of now trying to play the forex markets.
For the gamblers it will be the thrill of making a trade, perhaps the skill it entails but also of the chance to get a trade right and make a considerable amount of money. So there is definitely a trend towards someone who aspires to greater things and levels of luxury as well.
• PSD files only.
• The website should be clean, crisp modern in style.
• We have no "logo" and this should be created as part of the homepage design. Any design central text (logos for example) should be provided non-rasterized.
• If custom fonts are used these should be sent along with the design.
• If your design uses any library images – e.g. iStock - please provide both a link to the image (and ID) and the cost we'd incur in buying it should we should your design.
• Colour Palette – clear, clean and natural preferably using Web 2.0 colours.
• Screen Size – The website should fit a 1024px width display.
• Scrolling of page content should be avoided – or at least with a neat break between above the fold and below the fold content.
• Minimum Requirements. This project calls for a minimum of two pages to be created:
(1) the homepage design; and
(2) the review page template – onto this we will add content to review each online forex provider.
PLEASE NOTE - the review page template is as important as the home page design so please do not ignore it. Sadly any designs which do not have the second page (or otherwise meet the brief) will not be considered.
Any questions please just let us know!
Finserve Marketing


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12 Celebs Airbrushed to Look Bigger and Less Muscular
Case of the Disappearing Muscles
You're well aware of how Photoshop can brighten skin, erase cellulite, and slim tummies in order to transform supermodels into superhumans. But over the past few years, a new trend is taking over: reverse photoshopping, or using digital manipulation to make a person look bigger, less muscular, or even "healthier." Nicky Eaton, international director of communications at Condé Nast, has said many models in Vogue, GQ, Glamour, and others have been 'shopped to look fuller-figured.
"There have been cases where models are booked way ahead of a shoot and then they turn up two months later looking less healthy and perhaps a bit underweight. We wouldn't be happy showing them that way, so it is then that we would need that person to look a little bit fuller."
The editor of Self magazine agrees, admitting, "We retouch to make the models look bigger and healthier." But Robin Derrick, creative director of British Vogue, sums up the problem best, saying, "I spent the first 10 years of my career making girls look thinner—and the last 10 making them look larger."
Here are 12 examples of models and celebrities who've had their hard work in the gym airbrushed away.
Karlie Kloss
Supreme supermodel Karlie Kloss recently had rib removal, courtesy of a computer. But if thin is in, why edit her natural bone structure?
"We've become conditioned to expect perfected images of skinny, apparently boneless, smooth little girls in our magazines," says Jenna Sauers, a model and blogger at Jezebel.com. "In a certain way, we've come to rely on Photoshop to insulate us from the sharp reality of what maintaining an industry-approved fighting weight can do to a human body."
Kamilla Wladyka
Editor Jane Druker received international attention when she made
magazine's cover girl
Kamilla Wladyka
gain 30 pounds through the magic of airbrushing, taking her from "emaciated" to "healthy." Druker, the editor of Healthy, a mag sold in health food stores, explained her decision by saying the model "showed up at the shoot looking really thin and unwell."
Cameron Diaz
Once known for her curvaceous bod, in recent years Cameron Diaz has taken on a leaner, more athletic look—a decision some photographers don't care for, as evidenced by a photo shoot where the actress had her abdominal "v cuts" removed because they "looked too masculine."
Victoria Pendleton
Olympic cyclist Victoria Pendleton had her back muscles retouched to look less pronounced. The athlete and beauty columnist wrote in Zest magazine about the experience, "It still surprises me that we have such a narrow view of what makes women attractive. I've been photographed lots of times over the years, but one picture sticks in my mind. I wore a dress that exposed my whole back, and when I saw the photo on a screen at the shoot I thought 'Wow! My back looks muscly,' and I felt really proud. But when the picture was printed, my back was smooth and practically muscle free. They'd softened it all, and I was so disappointed because I'd put a lot of work into that! I guess, in their opinion, being muscly isn't that attractive in a woman. But surely if you take a picture of an athlete, you'd expect to see some muscle, wouldn't you?"
Lady Gaga
Lady Gaga is already known for her supernatural looks, but Vogue took the singer to new anthropomorphic heights when they digitally enhanced her hips and boobs on a recent cover. After a "behind-the-scenes montage of the shoot" was released by photographers Mert Alas and Marcus Piggott, Amy Odell of BuzzFeed wondered "why people bother with the original photography anymore when the end result is basically just a photo illustration." But the Lady herself was happy, tweeting, "Aah, the Vogue Express dropped off the September issue yesterday morning! I'm a cover girl and it's fab! Can't wait to show u tonight!"
Keira Knightley
Keira Knightley showed up on movie posters for her 2006 film King Arthur with more meat on her bones (particularly her arms and bust) than she really has. The famously petite actress was not impressed, saying, "Those things certainly weren't mine." And while she approved the pictures, telling the marketing department, "OK, fine. I honestly don't give a s—-," she has apparently learned her lesson. For her more recent movie The Duchess, "She has insisted that her figure stay in its natural state," an insider said. "She is proud of her body and doesn't want it altered."
Deutzen Kroes
Proving that even gorgeous lingerie models aren't immune, Deutzen Kroes recently had her pectoral and abs muscles "smoothed out" in a Victoria's Secret swimsuit shoot.
Candice Swanepoel
Fellow Victoria's Secret model Candice Swanepoel received similar treatment when she had abs definition and protruding hip bones photoshopped out.
The Queen of Pop has seemed to magically defy aging through her famously intense workout regimen and diet, but fashion house Dolce and Gabanna didn't appreciate the side effects of all her strength training during a recent ad campaign. Thanks to Photoshop, Madonna had almost all her hard-earned muscle tone and vascularity in her arms airbrushed off.
Demi Moore
In the past Demi Moore has made headlines for her nude pregnancy portrait on the cover of Vanity Fair and her Photoshop fail cover of W in which her hip was removed. The latest scandal to haunt the star? Her prominent chest and collar bones were erased in a cosmetics campaign for Helen Rubenstein. The actress lost a lot of weight after splitting with Ashton Kutcher so at the time of the ad campaign she was, according to industry insiders, "scary skinny."
Sarah Jessica Parker
When people aren't gushing over Sarah Jessica Parker's impeccable fashion taste or speculating about the state of her womb, they're talking about her amazing muscles. Unfortunately the actress recently lost her arm definition to retouching for an Elle magazine cover.
Kelly Ripa
Perky talk show host and known exercise enthusiast (she's been a SHAPE covergirl twice!) Kelly Ripa unfortunately lost a lot of muscle definition in a Fitness bikini shoot. But proving that nobody's perfect, she also lost her outie bellybutton in a Shape shoot.


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When I think of a snow day, I think about curling up in front of a roaring fire. Let's face it, I'm not a winter girl. In fact, one of my friends recently called me a Sunshine Girl and he was right – I love to be warm. So when faced with this month's Wine Blogging Wednesday theme (which I chose), all I could think about was port.
Port is the type of wine that warms me up from the inside. Port was originally a Portugese style of fortified wine. Under EU Guidelines, only port from Portugal may be called Port or Portugal. However, everyone makes it and outside of the EU, you'll often see "Port" used to denote a fortified wine. And no wonder port wine warms me up. It is fortified. Winemakers add a neutral grape spirit that stops the fermentation, leaves residual sugar in the wine, and ups that alcohol content.
So the other night, after braving some rather biting wind, I popped one of our Duraflame logs into the fireplace and we poured out a bottle of Lilly Pilly Estate 1995 Shiraz port.
This particular port is from Australia, not Portugal, but it honored the "Port" name well. At 18.5% AbV, it has a lower alcohol content than many of the ports we drink (which often top out around 20%). But it certainly went down fast and easy. Port, with the sugar and alcohol content, is a sipping wine, but the 375 ml bottle still disappeared quickly.
The Shiraz port had a lot of raisins and, surprisingly, rum on the nose. It's an obviously heavy wine and it was dark, almost chewy. We caught flavors of raisins, plums, and figs, as well as the obligatory dark cherry.
It was the perfect wine on a bitterly cold evening – a great way to round out a snow day!
Stay tuned for the Wine Blogging Wednesday Round-up – the complete list of everyone who participated – to show up early next week.
Still want to participate? Get your link to me by Friday, Jan 22 and I'll make sure you're included in the WBW round-up!
[…] brought out at the prospect of a snow day. I have to admit, I expected more Ports. Kevin & I indulged in a port here in the Cincinnati area, where snow is no stranger. Gary and Allison, recently of Cincinnati but now […]
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Krystal Pepper Memorial Scholarship Fund
Raising money for a scholarship fund in memory of Michelle's little sister who passed away in 2007


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Indicative Rate * AU$137.00 to AU$147.00
Panoramic Views : Rolling Hills : Magical Sunsets
The Block Vista B and B is the relaxing country escape you have been looking for.
Situated in the beautiful South Burnett on a 400 acre cattle grazing property and a two hour scenic drive from Brisbane and the Sunshine Coast.
There are three very comfortable Queen Bed rooms, two of which come complete with en suites. All rooms are located within and attached to the main homestead. Each room is very private, having its own separate entrance and facilities that will ensure your comfort throughout your stay.
Indulge in a scrumptious breakfast to start your day. Stroll around the property at your leisure and enjoy a delicious picnic lunch. Watch the spectacular sunsets by the dam with a glass of wine. Gaze at the stars in the endless night sky.
Spend time relaxing and revitalizing, or take a short drive and visit the many wineries and attractions around the South Burnett. Bring your bike and ride the Link Trail.
As you drive through the entrance gate and up the hill to the homestead, take a deep breath...feel your shoulders relax and let the vista's natural beauty embrace you.
Note: Information on listed products and services are provided by the operator and were correct at the time of publishing. Rates are indicative based on the minimum and maximum available prices of products and services. Please visit the operator's website for further information. All prices quoted are in Australian dollars (AUD).


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Diane Chevalier introduced Linda Keller, Gilmer Chapter Volunteer Coordinator for the Northeast Texas Child Advocacy Center who presented a program on the work of the Center. Ms. Keller discussed the various services provided by the Center such as a safe child friendly environment to children between the ages of 2-17 who allegedly have been sexually or severely physically abused or have been a witness to a violent crime and have been referred by a school or a law enforcement agency. She noted, "The children who come to the Center are allowed to tell their stories in a non-leading way to a trained forensic interviewer rather than having to repeat it multiple times to multiples agencies involved in the investigation and prosecution, therefore reducing the trauma to the child."
She stated that Upshur County has the highest number of abuse cases of all the counties they cover – Camp, Delta, Franklin, Hopkins, Rains, Titus, Upshur, and Wood. Keller noted, "When it comes to sex offenders, there are no stereotypes. A sex offender could be someone you know, someone who lives in your neighborhood, someone you would least expect. In 95% of all cases, the abuser is someone the child knows, possibly a relative, a friend, a neighbor."
The Center provides parenting classes and counseling in all of their locations. The satellite office In Gilmer is located at 302 E. Butler, Hwy 271. Hours are on Monday, Wednesday and Friday by appointment only. To schedule appointments, the Winnsboro location can be called 902-629-7588; email: email@example.com. At the conclusion of her presentation, sorority members gave a collection of items for use by the organization, i.e. socks, toothbrushes, clothing, toys, boxed juice, snacks, etc.
The program was preceded by a baked potato and salad meal provided by hostess Becky Starnes Attendees were Racheal Roberts, Becky Skinner, Donna Varnado, Becky Starnes, Barbara Vogl, Shelia Bledsoe, Dianne Wren, Shirley Wilson, and Diane Chevalier with guests Linda Keller and Yvonne Youngblood. The next regular meeting will be on Monday, February 24.


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The Crew make their first appearance in 2012 on national TV this Saturday on the new NBC Sports channel. The Black & Gold travel to Philadelphia to take on the Union Saturday at 3:30pm.
Click here to find NBC Sports on your local provider.
Official Crew Pub Tour stop this week at BBR in the Arena District. Learn More


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CSAIL Principal Investigator Harold (Hal) Abelson has been named the recipient of the 2012 SIGCSE Award for Outstanding Contribution to Computer Science Education. The award is presented annually in recognition of significant contributions to computer science education.
Abelson is the Class of 1922 Professor of Electrical Engineering and Computer Science at MIT and a fellow of the IEEE. He played key roles in fostering educational initiatives at MIT, including OpenCourseWare, which allows individuals worldwide to take MIT classes online, and DSpace, an online library for MIT research materials. Along with CSAIL Principal Investigator Gerald Sussman, Abelson developed MIT's introductory computer science subject, Structure and Interpretation of Computer Programs, which is widely credited with having a global impact on computer-science education at the university level.
The award will be presented at the 43rd SIGCSE Technical Symposium, where Abelson will be giving a plenary address.
For more information on Abelson's work, visit http://www.csail.mit.edu/user/1535.


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Africa Resists "Green" Revolution
[Africa: Agriculture]
The introduction of the new Alliance for a Green Revolution in Africa (AGRA) will have adverse social, economic and agricultural implications on the livelihoods of smallholder farmers who make the bulk of people on the entire African continent, opponents contend.
Agronomists, agro-ecologists, environmentalists and development experts who met in Mali at a conference on Climate Change, Agriculture, Fisheries and Pastoralism in Africa urged African government to reject the project which got a $150 million infusion from the Rockefeller Foundation and the Bill and Melinda Gates Foundation. The plan is to improve African seed and distribution over the next five years.
"AGRA ignores the many successful agro-ecological and non-corporate approaches to agricultural development," said Eric Holt-Gimenez of the Institute for Food and Development Policy, commonly known as Food First. "AGRA will undermine Africa's food sovereignty and kill its cultural diversity and agriculture."
AGRA was unveiled on September 12, 2006 by the Rockefeller and the Bill and Melinda Gates Foundations to improve seed hybrids, inorganic fertilizers, water management and agricultural extension services in Africa. The goal of this new agricultural project is to develop 100 new varieties in five years focusing on at least 10 different staple crops which include corn, cassava, sorghum and millet.
AGRA's programs are administered through the Programs for a Green Revolution in Africa (ProGra) which got an initial grant of close to $30 million for selected countries in East, Southern and West Africa.
Mariam Mayet of the African Centre for Biosafety said the officers of AGRA and ProGRA will initially be key senior staff from the Rockefeller Foundation who will be based in Nairobi, Kenya. The first major initiative of ProGRA, she said, is the Program for Africa's Seed System (PASS) intended to operate in 20 African countries. PASS will focus primarily on improvement and distribution of cop varieties, training of a new generation of plant breeders, seed distribution through seed companies, public community seed systems and public extension.
Provision of credit and training for small middle men agro-dealers for distribution of seeds, chemicals and fertilizers will also be done. Some agricultural experts say a "green revolution" merely include efforts aimed at increasing productivity of major food crops by incorporating scientific advances in plant breeding, development or expansion of supportive technologies and implementation of various reforms. They say large scale investment in irrigation, application of chemical fertilizers and other inputs and farm equipment all make up a "green revolution."
Proponents of AGRA say Africa missed the first Green Revolution and hence the need to embrace this new alliance project. They feel strongly that Africa missed out on the Green Revolutions that took place in Asia and Latin America in the 1960s and 1970s. AGRA is joining other multilateral institutions, G8 and other donor countries, international foundations and multinationals to invest in African agriculture.
Yet African scientists, agricultural specialists, farmers' organization and civil society activists are calling for a total rejection of AGRA as they cite several key concerns.
Opponents contend the growing influence of powerful multinationals such as Monsanto, Bayer, DuPont, Dow Chemical Company, BASF and Syngenta will lead to an extensive investment in genetically modified hybrids and "suicide" seeds which make farmers lose their indigenous and affordable seeds.
Monsanto, for example, controls 88 percent of global transgenic seed production. "There is not any other reality," said Holt-Gimenez. "This new Green Revolution is meant to perpetuate slavery and dependence on the major US seed companies. A new AGRA project in Africa means a new market for their seed."
"These multinationals are only speaking about profits and poisons. They are not speaking about the culture and needs of the Global South farmers," he said. "The wisdom of all farmers in the Global South and indigenous communities is not taken into account. They are promoting mono-cropping at the expense of promoting agro-biodiversity," he added.
Added Renato T Salazar, a South East Asia Regional Initiative for Community Empowerment (SEARICE) senior fellow:
"We must come out strongly and inform the political side and farmers about the badness of AGRA in Africa. The Green Revolutions that were implemented in Asia in the 1960s and 1970s had disastrous effects."
Opponents say beyond the yield gains from Green Revolution projects, there are many costs –economic. Agricultural and social. The use of large amounts of water, fertilizers and chemicals impoverishes the soil leaving it less fertile and heavily polluted. Apart from health hazards, there are also human health implications.
The biodiversity kept by farmers for ages is lost and this forces farmers to depend heavily on pesticides and seed supplied by multinationals. "The profound cultural and economic changes wrought by the Green Revolution of the 1960s and 1970s produced a massive rural exodus and with it, a profound loss of traditional knowledge and skills," Grain, a Food First publication reported.
"The Green Revolution is based on a scientific reductionism, which has resulted in monocultures, the use of chemicals, fertilizers and inappropriate mechanization. This is alien to Africa's peasant farming systems which pursues a more holistic approach to agriculture in which crops are combined with livestock, organic manure is used, soils are looked after and there is a deep respect for the wider environment."
"The Green Revolution is a serious threat to the continent's peasants, seeds and livelihoods," said Diamantino Nhampossa of Mozambique's National Peasants Union (UNAC) and Via Campensina in Africa. "Instead of recognizing the rich knowledge that peasant women and men have managed for millennia, the introduction of hybrid seeds and technological packages will further damage the peasants own production systems."
Renowned Ethiopian geneticist Dr. Melaku Worede said: "There's so much potential with seeds in Africa that is not being explored. It's being undermined by outside solutions. Rather than the industrial agriculture model, we should support more holistic approaches to agriculture. Farmer-led programs tend to look at more than just yields. There are about raising productivity without losing biodiversity."
Dr. Melaku Worede says: "We need to build from the strengths of the farmers. Our work shows that farmers are the best breeders and the best judges of new agricultural projects."
Tsiko is The Black Star News' Southern Africa correspondent based in Harare.
To comment or to subscribe to or advertise in New York's leading Pan African weekly investigative newspaper, or to send us a news tip, please call (212) 481-7745 or send a note to email@example.com
Also visit out sister publications Harlem Business News www.harlembusinessnews.com and The Groove music magazine at www.thegroovemag.com
"Speaking Truth To Empower."
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Colocalization Analysis
|Coloc_2 - Standardized, modular, Colocalization analysis. (ImageJ - Fiji - imglib)
|Daniel J White, Tom Kazimiers, Johannes Schindelin
|Daniel White
|on gitweb.
|Latest version
|Spring 2011
|Development status
|beta. Under development, but stable enough for real work.
- 1 Colocalization Analysis - using Coloc_2
- 2 Colocalization Analysis - What is Colocalization anyway?
- 2.1 There are two basic methods for colocalization analysis:
- 2.2 Open the sample data
- 2.3 Questions you should ask before attempting colocalisation analysis from 2 colour channel images, using the pixel intensity spatial correlation methods of Manders and Costes:
- 2.4 Check image data for problems and suitability for analysis
- 3 Notes and details on performing colocalization analysis (including using the old plugins)
- 4 Why scatter plots instead of colour merge images?
Colocalization Analysis - using Coloc_2
The first part of this tutorial is the basic user documentation of the Coloc_2 plugin which is found in the Analyze - Colocalization Analysis menu.
Coloc_2 implements and performs, the pixel intensity correlation over space methods of Pearson, Manders, Costes, Li and more, for scatterplots, analysis, automatic thresholding and statistical significance testing.
What is Coloc_2, and why?
Coloc_2 is an imageJ / Fiji plugin that uses the new imglib image data container library for image processing, and implements the above methods in a pixel data type (8, 16, 32-bit) independent, modular and easily extensible way. The source code has unit tests to detect if changes to the source code break the maths. It should be easy to add new methods since the plugin is designed with that in mind.
One main feature of Coloc_2 is the standardised PDF output, which is intended to make the results of different colocalization experiments comparable. Please tell us what you think should be in this standard output, so it is suitable to put directly into the supplemental info of a research publication.
This plugin supersedes the Colocalization Threshold and Colocalization Test plugins, which, unfortunately, were buggy and hard to maintain. So we started from scratch with a carefully planned and designed new plugin. The older plugins are now retired and not actively supported.
How to use Coloc_2
- Open images to analyse.
- you need a 2 colour channel image. If the image has more than 2 channels, identify the two you want to analyse with each other, then split the channels into separate images (Image- Color - Split Channels)
- If you want to analyse only some region of interest (ROI), there are 2 ways to do that:
- Select a region of interest with one of the imageJ selection tools, in one of the images.
- If the image is a z stack, then the ROI applies in all "slices" of the stack.
- You can have a third "binary mask" image, with the same x,y,z dimensions as the 2 images to be analysed:
- where the mask image is white (255 pixel value for an 8 bit greyscale image) colocalization will be analysed for those pixels only. Where it is black (zero pixel value), the pixels will be ignored: not included in the analysis.
- you can use a z stack as a 3D mask... its up to you how you make that mask image, manually or by some automated method.
- Select a region of interest with one of the imageJ selection tools, in one of the images.
- Launch the Coloc_2 plugin from the menu item: Analyze - Colocalization Analysis - Coloc 2
- or use the command finder: press L then start typing Coloc, then choose the plugin with the arrow keys and hit enter, or mouse click it to launch the plugin.
- In the plugin's graphical user interface (GUI) choose the 2 images you want to analyse in the first 2 drop down lists.
- Select the images according to which you want to be channel 1 and which to be channel 2.
- In the third drop down list selection, select the image/channel you want to use that has the correct ROI
- or you can choose a mask image - it must have the same xyz dimensions (number of pixels and slices) as the other 2 images.
- Choose which "Algorithms" are run and which statistics you wish to calculate, and if you want to save the "standardised" PDF result file, when the OK button is pressed.
- Turn the options on and off by clicking the selection button at the left of the Algorithm description
- Also choose the approximate size of the point spread function (PSF) in your images, and the number of iterations to run the Costes statistical significance test (We suggest a large number... the larger the number the longer the analysis will take. Do, at the very very least, 10 iterations (100 would be better).
- You should know approx. how big the PSF is (in pixels) in your images.
- If you dont, go back and read about what it is and why its important in colocalisation analysis (for instance read the Costes paper )
- This size determines what size of image chunks are shuffled in the randomisation process. PSF sized image pieces make physical sense, as thats the size of the smallest objects in the image.
- You should know approx. how big the PSF is (in pixels) in your images.
- Click OK to run the analysis.
- You will be asked if you want to save the results as a PDF file (if the Show "save PDF" dialog button was checked. So tell it where to save the PDF. This is standard output format, so you can compare with your friend, same vs. same (neat eh!?)
- Feel free to tell us what things you think should be included in this standardised PDF output file.
- You will be asked if you want to save the results as a PDF file (if the Show "save PDF" dialog button was checked. So tell it where to save the PDF. This is standard output format, so you can compare with your friend, same vs. same (neat eh!?)
Colocalization Analysis - What is Colocalization anyway?
Suppose you are given some images by a colleague, or have some images of your own, and you want to measure the amount of colocalisation between two of the dyes or stains in the images. First you have to define what you mean by colocalisation, and that is not trivial. One place to start reading about colocalisation and and for how to correctly capture quantitative fluorescence microscopy images suitable for colocalisation analysis, look here: Image Processing Courses at IFN Dresden
There are two basic methods for colocalization analysis:
- Pixel intensity Spatial Correlation Analysis (which is covered in this tutorial)
- Object Based Overlap Analysis (not covered in this tutorial, since it requires segmenting the image into objects and background, and thats a whole branch of image processing in itself).
At some point (preferably before you start), you should read these 2 papers very carefully: Manders et al. and Costes et al., since we are going to use these methods for the analysis in this tutorial. Don't be scared by the maths, its actually very simple :-)
Open the sample data
First, lets open a sample data set that we know should have very good colocalisation because the 2 subunits of a dimeric protein are stained with green and red dyes respectively. The methods of Pearson, Manders, Costes and Li should work very well for this sample, but maybe we can see some problems with the data? Maybe we can decide if the data is suitable for this analysis or not?
Open this sample data file colocsample1bRGB_BG.tif. Then use the "Image-Color-Split Channels" menu command to get a separate z stack for the 2 dyes (you can throw the blue one away!).
If you like, you can change the look up tables of the images (LUTs) so one is "green" and one is "magenta". Of course the colours here are always false. These false colours are only useful (to us as apes with trichromatic vision) to tell which channel is which: We are evolved to find red fruit in green trees. The optoelectronic detectors we use only see photons, and don't know what colour they are... that is determined by the fluorescence emission filters we use. There is no such thing as a green dye or a red dye, since they have broad emission spectra not a single wavelength corresponding to a certain "colour". If I want to show DAPI in green and EGFP as magenta, there is nothing "wrong" about that.
Questions you should ask before attempting colocalisation analysis from 2 colour channel images, using the pixel intensity spatial correlation methods of Manders and Costes:
- Q1) Is the image data noisy?
- Q2) Has there been lossy compression?
- Q3) Is the intensity information saturated / clipped / overexposed?
- Q4) Is there a problem with uniform background / detector offset?
- Q5) What is the spatial resolution?
Check image data for problems and suitability for analysis
To begin with, we should check the images for problems that might make the colocalisation analysis methods fail or be unreliable.
- 1) Significant noise (uncertainty in the pixel values - usually from detection of too few photons) means the methods we will use will significantly underestimate the true colocalization, or even completely fail to give the "right" result.
- 2) Lossy compression messes up the intensity information of the pixels, causing the colocalization result to be more or less wrong.
- 3) Intensity clipping/saturation is bad news. Pixel intensity correlation measurements rely on the pixel intensities being true and not clipped to 255 when they were really higher!. We did this in the DetectInformationLoss tutorial, so do that first if you didn't already!
- 4) We should look for wrong offset / high background (since this confuses the auto threshold method, since zero signal is not zero pixel intensity but some larger number, see below),
- 5) Spatial resolution)
The spatial resolution of the images, by definition, determines the spatial resolution that you are measuring colocalization at. The results will be different at different levels of spatial resolution. If the image is one big pixel, everything will colocalize!
The spatial resolution of the light microscope is limited by the wavelength of the light (and the NA of the objective lens) according to Ernst Abbe's work. Molecules / proteins are an order of magnitude smaller than the wavelength of visible light, so they could be many nm apart, but still appear in the same image pixel. Is that true colocalization? Maybe.... it depends how you define it!
Are the images spatially calibrated? If not then we need to calibrate them so we know the spatial sampling rate (think pixel or voxel size) in x, y and z. See the SpatialCalibration tutorial for how to do that. We need the images to be spatially calibrated in order for the Costes statistical significance test (below) method to work properly.
We need to think carefully about the correct or adequate spatial resolution in x, y and probably z. This depends on the Numerical Aperture of the objective lens. You can calculate the correct pixel or voxel sizes for the objective lens you are using, to get the maximum resolution that that objective lens can really see: Nyquist Calculator]. Essentially the pixels / voxels should be about 3 times smaller then the resolution of the lens. On the other hand, if you are only interested in larger objects, and not the smallest details the objective can see, it makes sense to have larger pixels or voxels. Again, these should be about 3 times smaller than the smallest feature you want to resolve.
Nyqvist tells us the spatial sampling should be about three times smaller then the smallest object we want to resolve. Remember, spatial intensity correlation analysis, as we will perform here, can not tell you that 2 proteins are bound together in some biophysical bonding interaction. However, it might suggest that the 2 molecules occur with the same relative amounts when they are present in the set of spatial samples (pixels or voxels) with intensities above the thresholds we will calculate below. In any case, it might be a hint that "maybe they are binding partners or in the same macromolecular complex". You SHOULD follow up by determining true binding using FLIM, FRET and biochemical methods like Immuno co-Precipitation etc.
There is another (badly behaved) sample data file here which we will use later: 150707_WTstack.lsm.
Notes and details on performing colocalization analysis (including using the old plugins)
Colocalization Threshold plugin: Note: this plugin is no longer under active development and support. Use Coloc_2 instead.
This plugin performs several functions for you in one go. With the "green" and "red" stacks of the colocsample1bRGB_BG.tif dataset open and the channels split (see above) choose the menu item "Analyze-Colocalization-Colocalization Threshold". Next select the right stacks for the analysis in Channel1 and Channel2. You can use a region of interest (ROI) if you like, which should be defined before you run the plugin. Check on "Show Colocalized Pixels" and "Show Scatter Plot" (see also Why scatter plots?), and others off. You can explore the options in Set options. Turn ALL the options on the first time you use it, so you see what it can do.
- It generates a 2D Histogram / Scatterplot / Fluorogram. this is a really good way to visualise the correlation of the pixel intensities, over all pixels/voxels in the image, and can tell you immediately about problems such as intensity saturation/clipping, wrong offset, emission bleedthrough (fluorescence signal from the wrong dye in the detection channel), and even if there are multiple populations of colocalising species with different ratios of dyes in the same sample. Think of it just like a FACS or Flow cytometry scatter plot; indeed it is very similar.
- It makes a linear regression fit of the data in the scatter plot. That is the diagonal white line in the scatter plot, the gradient of which is the ratio of the intensities of the 2 channels.
- It does the Costes method auto threshold determination. The thresholds are the intensity levels above which for both channels you say the two dyes are "colocalised". This method uses an iterative procedure to determine what pair of thresholds for the 2 channels of the scatterplot give a Pearson's correlation coefficient (r) of zero for the pixels below the thresholds. That means that all the pixels which have intensities above the two thresholds have greater than zero correlation, and the pixels below the thresholds have none or anti correlated intensities. The method is pretty robust (so long as you don't stupidly defeat it, i.e. with image data with high offsets / background), and is fully reproducible, meaning you will always get the same thresholds for the same data set, and similar thresholds for similar datasets. Threshold setting is a big problem in colocalisation analysis. If you use a tool that allows you to manually set the thresholds, obviously you can get any result you like, since you are subjectively deciding what is colocalised and what isn't. This might please your boss, but it's not very scientific is it? So don't do that! Use the Costes auto threshold instead! Some people say the Costes method sets the thresholds too low, and lower than they would set them by eye. That might be true, but manual methods are subjective and totally unreliable. The thresholds that Costes method sets always mean the correlation below the thresholds is zero. That's a good thing.
The plugin finally sends a bunch of statistics and results to the results window. You need to turn them all on using the "set options" checkbox of the plugin GUI. Some of these results are pretty uninformative. They are listed here, in arguably order of usefulness:
- The thresholded Mander's split colocalisation coefficients (zero is no colocalisation, one means perfect colocalisation. There is one coefficient per channel, which tells you the proportion of signal in that channels that colocalises with the other channel. Of course this might be different for the two channels! The thresholded Mander's coefficients are probably the numbers you would publish (not the Pearson's coefficients as these are less informative).
- The thresholds that were set by the Costes Auto threshold method.
- The Pearson's coefficients for: the whole image, image above thresholds (should be close to 1 for very good colocalizing dyes) and image below thresholds (should be around zero, because that's how the Costes auto threshold method tries to set them... so if it is not close to zero, something went wrong!)
- Linear regression solution: If the image data are close to ideal, with comparable mean intensity in both channels and no problems exist with offset/background, then by definition, the gradient of the regression line will be close to unity (1) and the intercept will be close to zero. These are both good things for the quality of the result. This information might be less useful in other situations, e.g. where there is only sparse data in one channel, or there are multiple colocalising populations of signals (several clearly independent patches/blobs in the scatterplot).
- The % volume and intensity colocalised values are pretty useless as they are very dependent on how much stuff and how much background there is in a certain image, and you will get totally different values for different images of the same sample, that happen to have different areas of background. In images that have no empty regions, like tissue samples, it might then be a useful number. Maybe % intensity above threshold colocalised might be a useful value in some cases?
Real problems and pitfalls using the Manders and Costes methods for colocalisation analysis.
- The auto threshold calculation method can fail if you feed it inappropriate information. That means it does not like images with high offset / high flat background, which adds a constant number to the "real proportional" intensity relationship to the "concentration" of the dye. If pixels that contain no real signal have large intensity values, the algorithm has no way of knowing that, and can reach a result for the thresholds where one or both of them is below the value of the lowest intensity value present in that channels of the image. This means that ALL of that channel's pixels are considered to be colocalised, then the Manders Coefficients that you get will reflect that aberrant situation. In these cases, the background / offset should be carefully removed/subtracted before running the Colocalization Threshold plugin. The images below are an example of this situation, using the badly behaved data set:150707_WTstack.lsm. Note that the vaules for M1 and tM1 are the same! This should not be the case. You can see the green channel threshold is wrongly set below the intensity where the image data actually starts :-O
- Effect of noise on Manders Coefficients. In the case of perfect colocalisation, where the intensities of the 2 channels are always the same: Low red with low green, and high green with high red, the scatterplot would have all the data point falling on a straight diagonal line, since the green intensity would always bee the same as that of the red! however, that is the ideal case and real biological data is noisy! Noise (be it from the dyes not staining every single molecule, or from statistical photon shot noise from recording the signal from too few photons, or from other noise sources) will cause the pixel intensities to deviate from the perfect case, to be lower or higher than they really are on average. This causes scatter in the distribution of the data point in the scatterplot perpendicular to the line of regression fit. So you can see the noise by looking at how spread or tight the scatterplot points are. Also, since Manders coefficients are measuring correlation, and noise lowers the similarity of two identical signals, noise lowers the Manders coefficients to less than they should be for an image with very low noise. So for the same object under the microscope, a nosier images will appear to give less colocalisation than a clean low noise image. That means you cant compare different images with different signal:noise levels, unless you have some way of estimating the noise and correcting for it.
- Fluorescence Emission Bleed Through Looks Like Perfect Colocalisation!!! As is often true for DAPI nuclear stain and GFP dye pairs, when images are captured at the same time, with both dyes being excited and detected simultaneously, fluorescence emission bleed through gives misleading results, as the signal from the DAPI also appears also in the GFP detection channel! Where there is more DAPI, there is also more signal in the GFP channel. This looks like really good colocalisation, but of course it is totally false! It is a problem with the imaging systems not being set up correctly or not used correctly. This can also happen with many other dye combinations, if they have overlapping emission spectra. Always check your spectra. You can do that here: Invitrogen Fluorescent Dye Spectra Viewer]. To be safe, check your emission filter sets don't allow in the wrong signal, and do "sequential imaging", so you only excite and image one dye at a time.
- Next is the topic: Regions of Interest (ROIs), and whether or not to consider zero - zero pixels as part of the interesting data for the algorithms to deal with. If you think about it, in a fluorescence image there is typically quite a large area which is black in both channels... for instance where there is space between cells, or just no signal in either channel since that area is not part of an interesting area of the sample. A philosophical point... but a significant one: Why bother taking images of black areas? Why bother analyzing black areas for colocalisation? Surely you are not interested in those regions, as they contain no information of use to you? If you perform these pixel intensity correlation methods and include zero zero pixels, then of course these pixels have a very high correlation! They have the same value. But they are totally uninteresting! Sure, the auto threshold method excludes them from the tM1 and tM2 figures, but why include them in the first place? Probably better not to include them unless there is a good reason to do that. Why not just image the area, or just analyze the area where your biology is happening? If you analyse an image with large areas of close to zero and zero intensities, then the autothreshold method will tend to lower the thresholds to include more of that non interesting background. If you image the same sample, but only image a patch of the interesting part, say cytoplasm, then the autothreshold will probably give higher thresholds, and exclude more non interesting background, so the thresholded Manders coefficients will better reflect the biologically interesting parts of the image data - right? You can analyze only a region of interest by making an ROI then selecting the use ROI option in the plugin. You can use a regular shape (rectangle or ellipse) or even a freehand ROI to manually select the interesting part of the image and ignore the part you know is background. Yes, this is a subjective decision, so be careful! You can see in the following example screenshot that for the same misbehaving data set, using an ROI which roughly gets just the cell, the thresholds were calculated properly and the tM1 and tM2 are sensible and lower then 1.00:
- Colocalization Test plugin: Note: This plugin is no longer actuvely developed or supported. Use Coloc_2 instead.
To perform the Costes test for statistical significance (which you should ALWAYS do after calculating the thresholded Manders coefficients and the scatterplot), choose menu item: "Analyse-Colocalization-Colocalization Test"
Choose the correct Channel 1 and Channel 2 images stacks from the drop down lists. Make sure "Current Slice Only" is off, and "Keep Example Randomized Image" and Show All R values" are on. Then click "OK"
The results window will then display the calculated P-value, and some other details of the test calculation.
The Costes method for [Statistical Significance] relies on the spatial calibration of the image, knowledge of the [Numerical Aperture (N.A.)] of the objective lens, and the fluorescence emission wavelength to calculate how many pixels the point spread function covers in the image. Then it takes the image in one of the channels, and randomizes it by moving PSF sized chunks of the image to random locations in a new random test image. Then it calculates the [Pearson's correlation coefficient (r)] between the randomized image and the original image of the other channel. If the correlation of the randomized image with the real image of the other channel is as good as or better then the correlation between the two real images, then any correlation that you measure is no better then what you would have got by chance for this image. This test is performed many (100) times, and the P-value is output, which is the proportion of random images that had better correlation than the real image. A P-value of 1.00 means that none of the randomised images had better correlation. 0.95 is the normal statistical confidence limit of 95%. Anything lower than that, and the correlation / colocalisation that you measure in the real images is not likely to be better than random chance, and thus is probably not interesting.
In this case the P-value should be 1.00. Since you told it to display the Pearson's correlation (r) values (R values here), they are in another window. You can see they are all close to zero, and in the results you can see that on average the randomized R value is about zero, meaning that the randomized images all had no correlation with the real image. Which is a good thing!
Other available methods, such as Fay and Van Steensel, do the same thing but randomize the image in less rigorous but very simple ways, which may lead to errors such as over or under estimation of the P-value. However, they are faster than the Costes method.
Again, as for the Colocalization Threshold plugin, using an ROI here may well make very good sense, as you are only interested in the correlation between the 2 colour channels in parts of the image where the biology you are interested in is located. Background its typically uninteresting, and you can exclude it from the analysis. Its probably sensible to to use the same ROI as you used in the Colocalization Threshold plugin!
Why scatter plots instead of colour merge images?
Far too often, composite/merge images of red and green channels are considered sufficient to demonstrate colocalisation. This is plain wrong. The problems with red/green merge images for colour-blind people aside, there is another very good reason to require scatter plots: the perception of human eyes and brain can be fooled very easily. Just have a look at this image:
Most people might think that the prominent spirals are in shades of green and blue, but they actually have exactly the same color! You can verify this yourself by calling File>Open Samples>Spirals (Macro) in Fiji.
So... now, how do you feel about determining colocalization by looking for yellow blobs? Doesn't make much sense does it? You need to measure something, not simply subjectively "look at" a red/green colour merge image.
An even beter reason to always look at scatterplots / 2D histograms / cytofluorograms is that they actually show the thing you are looking for and talking about - the correlation (or not) between the intensities of the 2 colour channels of the pixels over space.


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Microsoft falsely branded BBC CBeebies, CNN.com and other websites as Windows 8 piracy haunts - and ordered Google to remove them from search results.
Pages belonging to the Beeb's children's telly service CBeebies, film reviews site Rottentomatoes and US cinema chain AMC Theaters - as well as web articles by the BBC's technology news department, CNN and The Huffington Post - were among 66 URLs Microsoft wanted to block. The Windows 8 giant wielded the US's Digital Millennium Copyright Act (DMCA) to censor the search results.
Some of the targeted pages, such as the BBC's, remain in Google's results as the search giant has whitelisted trusted domains against automatic take-downs - however the others weren't as fortunate.
The take-down request appears to have been issued by mistake and the URLs generated by software operating on behalf by Microsoft. The copyright-infringement-hunting bot coughed up roughly 800 URLs on 27 July, a list of which has just been made public on the law-watching website Chillingeffects.
A number of websites caught in the erratic software's sights included torrent file-sharing hangouts. But Microsoft's bot also accused various innocent websites of pirating copies of Office, Xbox 360 and Visual Studio as well as a beta version of Windows 8.
The number of take-down requests Google is reported to have received has mushroomed in 2012: in August the search giant was asked to drop 1.5 million URLs from its results each week compared to 131,577 in the same month last year.
Censorship requests come from governments, interest groups and individuals; under the DMCA, copyright holders can demand the removal of links to sites that infringe copyright. Famously, Viacom sued YouTube owner Google in 2007 for hosting its videos - and the lawsuit was stalled when Google removed the offending footage, but was reignited when a US appeals court sent the case back to the lower court after siding with Viacom and agreeing that YouTube had turned a blind eye to the infringing material in the first place. ®
Sponsored: How to simplify data protection on Amazon Web Services


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Monday - Friday
All visits are scheduled by appointment only.
Krisha McCoy, MS
Aphasia is a disorder that affects the ability to communicate. People with aphasia may have difficulty with the expression and/or understanding of language, as well as reading and writing. Aphasia can be classified into 2 broad categories.
Aphasia is caused by an injury to parts of the brain that are involved with language. The injury may be the result of:
Aphasia is more common in older people. Other factors that may increase your chance of aphasia include:
Aphasia is a symptom of an underlying problem. It may include:
You will be asked about your symptoms and medical history. A physical exam will be done.
If you have a brain condition, you may already be seeing a doctor who specializes in the nervous system. This doctor will most likely be able to recognize your aphasia. Some simple tests may be done. For example, you may be asked to follow commands, answer questions, name objects, and have a conversation. You may then be referred to a speech-language pathologist who will perform additional tests to assess your speech and language skills.
Imaging tests are used to evaluate the brain and other structures. These may include:
Your bodily fluids may be tested. This can be done with:
Your brain activity may be measured. This can be done with
You may also be given the following specialized tests:
Talk with your doctor about the best treatment plan for you. Treatment will focus on:
Options for treating aphasia itself include:
A speech-language specialist will help you:
This therapy may take place in both individual and group settings.
A speech-language therapist will help you and your family learn how to best communicate with each other.
Psychological evaluation may also be helpful.
Since stroke is a common cause of aphasia. To help reduce the chance of a stroke:
If you have signs of a stroke, call for emergency medical services right away.
National Aphasia Association
National Institute of Neurological Disorders and Stroke
Aphasia Institute
Brain Injury Awareness
Aphasia. American Speech-Language-Hearing Association website. Available at:
February 16, 2018.
Aphasia. National Institute on Deafness and Other Communication Disorders (NIDCD) website. Available at:
Updated March 6, 2017. Accessed February 18, 2018.
Stroke rehabilitation. EBSCO DynaMed Plus website. Available at:
http://www.dynamed.com/topics/dmp~AN~T580145/Stroke-rehabilitation. Updated January 19, 2018. Accessed February 16, 2018.
Last reviewed February 2018 by EBSCO Medical Review Board
Rimas Lukas, MD
Please be aware that this information is provided to supplement the care provided by your physician. It is neither intended nor implied to be a substitute for professional medical advice. CALL YOUR HEALTHCARE PROVIDER IMMEDIATELY IF YOU THINK YOU MAY HAVE A MEDICAL EMERGENCY. Always seek the advice of your physician or other qualified health provider prior to starting any new treatment or with any questions you may have regarding a medical condition.
Copyright © EBSCO Publishing. All rights reserved.


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Furniture Taken To Room Of Choice
Removal of old furniture
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Why Ash Logistics For Furniture Delivery To And From Aberdeen?
- Our White glove delivery comes as standard with all of our furniture delivery, see below for more details.
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Ash Logistics, furniture logistics specialists, are always ready to take your call. No waiting about, no annoying phone queuing systems, just people who want to help people.
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Total posts: 1
Not sure if this site is where to meet a compatible date/ partner seems kinda slezzy . Is any one really here for a soulmate?
I agree with Jensen I have seen worth websites and think that this one might even be quite decent.
Though I don't know what to think of online dating in general as it seems extremely visual. I know that's known of us men but too often and from many people too I heard that women don't really care about the look of a guy but when it comes to this online thing I figured that it doesn't matter what's written in one's profile if they don't like your pictures.
I think people have greater expectations online due to the vast offer of meat. Therefore they tend to be even pickier than they are in the "real" world. I alos think that these websites are often used by people to evaluate their attraction or the like. Especially women if I may say so.
Proof me wrong.
Total posts: 6
Hi saw your post it is definetly a weeding out process. there are nice folks everywhere you just have to look hard enough.
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Profit and Economic Growth
Many facts attest to the existence of close ties between producer profits and economic growth. In the first place, in periods of economic growth, an overwhelming number of enterprises have fairly high profits. The markets are dominated by general confidence and optimism. A wholly different picture can be seen when economic growth is absent. In crisis conditions, producer profits tend towards zero, a few enterprises have insignificant profits and others suffer losses. In the second place, profits serve as the main source of investments, and as such the magnitude of economic growth depends on the amount of profit. If loans are a source of investment, then the guarantee for its repayment is precisely profit. The amount of loans received is directly dependent on the size of profits. In the third place, in a simple, commodity exchange economy the sole potential for generating profits is acquisition of additional commodities. It follows that the total profit of all economic agents is precisely equal to the cost of additionally manufactured commodities. As such, total profit equals economic growth. In such an economy, the words "profit" and "economic growth" practically become synonyms. If it can be proven that the stated equality always occurs, in any economy, then it will become possible to explain in a fairly simple manner the cyclical nature of economic development, to understand the reason for the existence of interest, and to continue drawing together the two branches of economic science: micro- and macroeconomics.
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DOI: https://doi.org/10.5296/ber.v2i2.2285
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Mother Encourages 2 Girls To Brawl It Out! (Female Pounds Another Chick's Head On Hood Of Car) "Hell No. Not On My Car"
Mother says not on her ride
Please click the "Report" button below if the video on this page is not working properly.
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Artwork description:
To create this pleasant piece, I used a highly pigmented gouache and mixed media. The colors are rich, bright, vibrant and make the artwork truly standout. This piece of unique art will embellish your home, in your reading room, by the side table or on your desk.
The piece comes unframed signed on the back and varnished for protection against the environment.
*Shipment prices are for paintings that do not exceed 5 Kg (10 pounds). My work usually does not exceed 5 kg.
*The colors of the photos can differ slightly from those on the original piece of artwork.
Excess postage will be reimbursed promptly.
#sand dunes and sunrise #woman with shorts #woman by the beach #landscape #seascape #expressive #landscape painting #ocean #seaside #blue sky #lake district #grey sky #gestural #blue sea #sand dunes
I long for you (Souvenir postcard series) Seascapes (2018)
Mixed-media painting by Samacca Art
- Mixed-media painting on Paper
- One of a kind artwork
- Size: 9.06 x 11.81 x 0.39 in (unframed) / 9.06 x 11.81 in (actual image size)
- Signed on the back
- Style: Expressive and gestural
- Subject: Landscapes, sea and sky
This artwork is sold by Samacca Art from Germany


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There can be few businesses that do not understand the value and importance of social media. Having a presence on the likes of Facebook and Twitter can help to put forward a friendly face and gives customers and other businesses a handy point of contact. But managing social media can be a nightmare. This is where BuzzBundle aims to help out.
Things get off to a great start thanks to the fact that the software is available for Mac, Windows and Linux. There are currently not mobile versions available, but this is not necessarily a major problem. The software lets you create a profile that can then be used to manage numerous related accounts. Your business may have a Facebook and Twitter account that are used by customers, while the corporate blog and LinkedIn profile are more likely to be accessed by clients, competitors and those in the industry.
Even in this scenario, having four different profiles to manage can quickly spiral out of control. One of the things that BuzzBundle lets you do is to manage them all from one place, without the need to keep signing in and out of different accounts. Different accounts can be grouped together into related personas, each reflecting a different side of your business, and in this way it is possible to set up target campaigns complete with scheduled posts.
But the tool can also be used in other ways. It can be used to monitor social networks, blog and forums for any references to you, your business and your products and services. This makes it possible to quickly respond to complaints and queries even if they have not been made directly to you or your company.
You are also able to create new social network accounts as and when required from within the app. Decide that you need new profiles for a new branch of your business? BuzzBundle can be used to fill in all of the necessary forms so you don't need to visit countless websites.
It is BuzzBundle's monitoring options that are particularly interesting. It helps to automate the process of tracking your company's online rating, popularity and more. It provides a handy way to stay in control of your brand reputation and more easily keep in touch with clients and customers with a minimum of effort and outlay.
There is a free version of BuzzBundle available and, as you would expect, this features a few limitations. While it is possible to work with an unlimited number of social profiles, you are only able to monitor a percentage of online mentions of your company or brand, can monitor fewer keywords and there is no option to save your online history. All of this, and more, can be unlocked in the paid for version.
A staggeringly powerful social media management tool that will help propel any company to the next level by simplifying the control of an online presence and allowing for better engagement with customers and clients.


text: Hello there! I'm an an undergrad in University of Brunei Darussalam taking up BSc. Computer Science. I'm currently working on a project that deals with java programming and I'm really really noob at it. So I'm hoping I could get help here when I'm stuck and likewise learn something here too.