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Adam sings without a band behind him and the venue doesn't appear to support the performance with the same level of professionalism as he's accustomed to. There are constant sound issues and the engineer is unresponsive to Adam's gestures so he has to interrupt his performance to give verbal instructions. As you can tell, this isn't one of my favourite gigs because I think this is one of the rare occasions on which Adam seems a little uncomfortable. It's like he and the audience - which isn't made of avid fans - aren't quite on the same level and that energy exchange he often mentions is somewhat stunted. He has to work extra hard to engage them and get them going. I feel his frustration that they don't afford him enough respect and he has to tell them to shut up at the start of BtbW.
All videos from this concert are now in this embedded playlist:
Despite all this, the vocals are brilliant throughout and the banter is charming. I love this longer version of RoF and this is the only time we hear him sing both BoW and BtbW outside of AI. It's been a while since we last heard this slow emotional version of MW and he manages to recreate those stratospheric notes of BtbW with aplomb. We get a triple helping of 'Baby', 'Woman' and 'Man' at the a capella ending of a high-energy, gyratory, rocking WLL. I miss this arrangement and would love to hear it before the end of the tour. It's hard to believe he does all of this whilst wearing fangs. Yes, he can still fucking sing amazingly well with big fangs in his mouth!
Get my Halloweeny Glam Nation design in velvety print on a T-shirt:
And here's a new Glampire pic from last night:
Happy Halloween!


text: Medicine https://hdl.handle.net/2123/5412 2020-06-07T00:50:34Z 2020-06-07T00:50:34Z Harvest: an open-source tool for the validation and improvement of peptide identification metrics and fragmentation exploration McHugh, Leo C Arthur, Jonathan W https://hdl.handle.net/2123/6443 2012-04-29T23:46:28Z 2010-01-01T00:00:00Z Harvest: an open-source tool for the validation and improvement of peptide identification metrics and fragmentation exploration McHugh, Leo C; Arthur, Jonathan W Background// Protein identification using mass spectrometry is an important tool in many areas of the life sciences, and in proteomics research in particular. Increasing the number of proteins correctly identified is dependent on the ability to include new knowledge about the mass spectrometry fragmentation process, into computational algorithms designed to separate true matches of peptides to unidentified mass spectra from spurious matches. This discrimination is achieved by computing a function of the various features of the potential match between the observed and theoretical spectra to give a numerical approximation of their similarity. It is these underlying "metrics" that determine the ability of a protein identification package to maximise correct identifications while limiting false discovery rates. There is currently no software available specifically for the simple implementation and analysis of arbitrary novel metrics for peptide matching and for the exploration of fragmentation patterns for a given dataset. Results// We present Harvest: an open source software tool for analysing fragmentation patterns and assessing the power of a new piece of information about the MS/MS fragmentation process to more clearly differentiate between correct and random peptide assignments. We demonstrate this functionality using data metrics derived from the properties of individual datasets in a peptide identification context. Using Harvest, we demonstrate how the development of such metrics may improve correct peptide assignment confidence in the context of a high-throughput proteomics experiment and characterise properties of peptide fragmentation. Conclusions// Harvest provides a simple framework in C++ for analysing and prototyping metrics for peptide matching, the core of the protein identification problem. It is not a protein identification package and answers a different research question to packages such as Sequest, Mascot, X!Tandem, and other protein identification packages. It does not aim to maximise the number of assigned peptides from a set of unknown spectra, but instead provides a method by which researchers can explore fragmentation properties and assess the power of novel metrics for peptide matching in the context of a given experiment. Metrics developed using Harvest may then become candidates for later integration into protein identification packages. Source code for Harvest 1.0 2010-01-01T00:00:00Z Single Nucleotide Differences (SNDs) in the dbSNP database may lead to errors in genotyping and haplotyping studies Musumeci, Lucia Arthur, Jonathan W Cheung, Florence SG Hoque, Ashraful Lippman, Scott Reichardt, Juergen KV https://hdl.handle.net/2123/5419 2010-09-02T03:12:12Z 2009-01-01T00:00:00Z Single Nucleotide Differences (SNDs) in the dbSNP database may lead to errors in genotyping and haplotyping studies Musumeci, Lucia; Arthur, Jonathan W; Cheung, Florence SG; Hoque, Ashraful; Lippman, Scott; Reichardt, Juergen KV The creation of single-nucleotide polymorphism (SNP) databases (such as NCBI dbSNP) has facilitated scientific research in many fields. SNP discovery and detection has improved to the extent that there are over 17 million human reference (rs) SNPs reported to date (Build 129 of dbSNP). SNP databases are unfortunately not always complete and/or accurate. In fact, half of the reported SNPs are still only candidate SNPs and are not validated in a population. We describe the identification of SNDs (Single Nucleotide Differences) in humans, that may contaminate the dbSNP database. These SNDs, reported as real SNPs in the database, do not exist as such, but are merely artifacts due to the presence of a paralogue (highly similar duplicated) sequence in the genome. Using sequencing we showed how SNDs could originate in two paralogous genes and evaluated samples from a population of 100 individuals for the presence/absence of SNPs. Moreover using bioinformatics, we predicted as many as 8.32% of the biallelic, coding SNPs in the dbSNP database to be SNDs. Our identification of SNDs in the database will allow researchers to not only select truly informative SNPs for association studies, but also aid in determining accurate SNP genotypes and haplotypes. This file contains a complete list of single nucleotide differences (SNDs) identified in the research described in the paper: "Single Nucleotide Differences (SNDs) in the dbSNP Database May Lead to Errors in Genotyping and Haplotyping Studies" Lucia Musumeci[1,6,*], Jonathan W. Arthur[2,3,*], Florence SG Cheung[1], Ashraful Hoque[4], Scott Lippman[4], and Juergen KV Reichardt[1,5] [1] Plunkett Chair of Molecular Biology (Medicine) Bosch Institute The University of Sydney Medical Foundation Building (K25) 92–94 Parramatta Road Camperdown, NSW 2006 Australia [2] Discipline of Medicine, Sydney Medical School The University of Sydney Camperdown, NSW 2006 Australia [3] Sydney Bioinformatics The University of Sydney Camperdown, NSW 2006 Australia [4] The University of Texas M. D. Anderson Cancer Center Houston, TX 77030 USA [5] Corresponding Author: email@example.com [*] LM and JWA contributed equally to this work [6] Current Affiliation: Immunology and Infectious Diseases Unit, GIGA-R, Liège University, Liège, Belgium This paper has been accepted for publication in the journal Human Mutation, September 2009. Each row of the data file corresponds to a reported SNP in the dbSNP database subsequently identified in the paper as a SND. The columns contain the following information (in column order): 1) The RefSNP id (rs#) for the SND. 2) The base pair position of the reported polymorphic residue within the full sequence up- and down-stream of the SNP contained in the dbSNP database 3) The reported polymorphic alleles 4) The number of times the SNP and its surrounding sequence aligned to the genome within the criteria of sequence identity and sequence coverage defined in the Materials and Methods section of the paper. 5) Whether this SNP has been identified as a SND or not. (Note: this data file contains only SNDs, so all entries in this column are listed as "SND") 6) The heterozygosity of this SND as reported in the dbSNP chromosome report. The quantity is used to determine "very strong" or "strong" subgroups of SNDs according to the procedure outlined in the Materials and Methods section. 7) The standard error of the heterozygosity of this SND as reported in the dbSNP chromosome report. This quantity is not used in this study. 8) The maximum reported probability of this SND being real as reported in the dbSNP chromosome report. This quantity is not used in this study. 9) The validation codes of this SND as reported in the dbSNP chromosome report. The quantity is used to determine "very strong" or "strong" subgroups of SNDs according to the procedure outlined in the Materials and Methods section. 10) The positions in the genome sequence where the SND aligns. This is a string containing multiple entries separated by a pipe (|). Each entry (position) includes four pieces of information, separated by colons. This information is: * The chromosome number to which the SND aligns * The strand the SND aligns to, where 1 = sense and -1 = antisense * The base pair position to which the SND aligns in the chromosome * The allele found at that position 2009-01-01T00:00:00Z

subset: commoncrawl


text: TV / Video Cops Ousted by Fox After 25 Seasons, Moves to Spike TV Police reality show will have new episodes on guy-centric cable netBy Sam Thielman|May 6, 2013 Share By Sam Thielman|May 6, 2013 Share Cops, the great-granddaddy of the contemporary reality show, finally got its walking papers from the Fox broadcast network after 25 seasons, only to get picked up immediately by male-centric cable network Spike TV. The show has been on the air constantly since 1989 and remains ubiquitous in syndication on TruTV, G4 and several other outlets abroad. It also began life as one of the cheapest shows on broadcast, since it was shot entirely on video and requires only the cooperation of local police departments to make an episode. Aspects of the series, especially the grainy video and the theme song (Bad Boys, by reggae band Inner Circle), have been featured in dozens if not scores of parodies, including on Fox itself. Orders for the show sometimes reached as many as 45 episodes in a season. All in all, more than 900 episodes of Cops have aired, "a portion" of which will be available to Spike (though not all) according to the network. The number of new episodes ordered was also undisclosed. The acquisition by Spike is driven principally by the show's continued popularity in the demo, and by its preexisting relationship with John Langley, who created both Cops and Spike's own Undercover Stings. "Cops is a remarkable series that has been able to sustain strong ratings well into its third decade, a monumental achievement in television," said Spike president Kevin Kay in a statement. "As we continue to grow and expand our audience, new episodes of Cops, with its loyal audience of adults 18-49, is the perfect addition to our prime-time lineup on Saturday nights." In recent days, the show has come under fire from African-American advocacy group Color of Change, which exhorted advertisers to put pressure on executives not to renew the series, claiming that the show portrayed black people—especially poor black people—in a negative light. "With such a narrow range of black characters and personalities in primetime, the negative perceptions and distorted images presented by shows like Cops create an atmosphere of suspicion that desensitizes and conditions audiences to view harsher punishments and police misconduct—including police brutality and unconstitutional searches—as acceptable," read an email from the group last week. Color of Change is celebrating the move to Spike, calling it a "victory." Share http://adweek.it/10CPdIH copy Adweek Adweek


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