text:
c1sc0 brauchte ein neues Anderes Design und hat einen Wettbewerb auf 99designs gestartet.
Aus 25 Designs von 5 Designern wurde eine Gewinner gewählt.
I'm an iPhone/Web developer & I'm about to launch a new product. I'm afraid I can't tell too much right now, suffice to say that it'll be in the social networking sphere.
To celebrate 99designs' Webby Award I thought I'd give back to the community by throwing a fun & well paid contest!
Isn't drawing cartoons pure unadulterated FUN? That's exactly what I am asking you to do! & I'm paying you for it too!
I've got the cartoon's script & text nailed down but my drawing skills are so bad a 6-months old could do better!
I have attached a bad drawing of what the cartoon should look like & described each frame below.
Feel free to be creative & go wild inside each frame in the cartoon, but you NEED to follow the layout & at a minimum you need to draw what is described in each frame.
I'll upload the finished & winning cartoon for all to see! Complete with text & all ;-)
Do you like TheOatmeal? (http://www.theoatmeal.com) ... ThingsBearsLove? (http://www.thingsbearslove.com) ... Xkcd? (http://www.xkcd.com) ... Cyanide & Happiness? (http://www.explosm.net/comics/2091/) ... We the robots? (http://www.wetherobots.com/) ... ?
Yep, me too ... that's why I need YOU to draw my cartoon. With YOU I mean someone who:
1) Is creative
2) Can draw cartoons in a simple & clear style
3) Can make the drawings funny
I want you to draw a cartoon that :
1) Is fairly WIDE (think 800px)
2) Is BIG (making the user scroll is fine!)
3) Is Vector-based
4) Has a clean, simple design
5) Has a white background
6) Has a unique, distinct drawing style
7) Has BIG speech bubbles (I will add text later)
8) Has LOTS of whitespace (I will add text later)
So ... are you up for it? Then open the attachment & read on!
!!! Open the attached File & Ask Questions if you don't understand !!!
Delivery = psd or illustrator
Frame 1: Shows the equation W = Jp / s ... you can pick any kind of setting (classroom, whatever)
Frame 2: Shows a campfire ... again ... you can pick a setting (boyscouts, whatever)
Frame 3: Sunshine radiating on earth. This sentence can be your inspiration: "Did you know that the sun shines 174 PetaWatts of power on our little planet? "
Frame 4: Lots of barbecues flying to earth. This sentence can be your inspiration: "That's like 348000 barbecues hitting earth every second!"
Frame 5: Shows the equation W = Jh / s ... again, ... you can pick any kind of setting (classroom, whatever)
Frame 6: Come up with a funny frame about *sweat*. This sentence can be your inspiration: "Think sweet sweet sexy sweat!" ... (keep it decent but sexy)
Frame 7: Shows two chickens making love in a funny, decent, censored way. This sentence can be your inspiration: "Did you know a good session of dancin' the funky chicken burns almost 100 Kcal?"
Frame 8: Shows a hamburger with 1/3 bitten/sliced out of it. This sentence can be your inspiration: "This means you should have sex three times for each cheeseburger you eat!"
Frame 9/10: Shows 2 todo list, first one is 'bored', second is 'happy'.
Frame 11/12: Shows people talking, in one the people are happy, in the other people are bored.
Frame 13: People talking, Twitter & Facebook logo talking, happy
Frame 14: Stock market crash caused by greedy math / computer nerds. Show them wasting money!


text:
Know what device every visitor is using to visit your website.
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51Degrees provides deeper insights into user preferences and device-specific behaviour, for mobile network operators and their suppliers.
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Discover how your customers are viewing your website - what device, what screen size…
Learn how your website performs and discover great insights into how customers behave based on their device.
Richer content, better user experience, increased engagement.
Identify the connection speed and quality of every website visitor.
Deliver a better user experience on your website, by tailoring your content based on your customer's connection.
Great user experience, reduced shopping cart abandonment, better performance.
51Degrees legal terms and conditions.
The ability to quickly segment audiences, target appropriately and optimise ad creative based on accurate analytics is vital for Adtech companies.
51Degrees' Device Intelligence can help you do this with it's patented technology, unrivaled accuracy and industry leading speed.
Use Device Intelligence to take your business to a whole new level.
You have invested in advertising in all the right channels, why fall at the last hurdle.
Leading advertisers use device intelligence to ensure they understand every user before delivering digital content and experiences.
51Degrees ensures your website doesn't get in the way of your brand conversations.
Publishers mobile sites often have huge page load times which results in abandoned page visits and ads not being viewed.
51Degrees enables content and advertising requests to be optimized for every user's device.
Improve user experience, drive engagement and increase advertising revenue with 51Degrees Device Intelligence.
Responsive web design is not a one size fits all solution.
With money and man hours spent on advertising don't let a poor user experience compromises a potential conversion.
Implement 51Degrees server-side to optimize content faster, resulting in an improved user experience and increased conversion rates.
Leading digital agencies need to keep up to date with the latest trends and tool sets to ensure your clients get the best results can be a challenge when you are busy managing the day to day demand from clients.
Using 51Degrees you can adapt online content to maximise the potential of online conversions and steer the end user purchase path.
Plugin us into yours and your clients' web platforms and see a more granular level of detail about the devices used for clients site.
Your platform is dependent on accurate device data to support improving client-side performance by optimizing user experiences server-side
51Degrees provides the fastest and most accurate device information, including 217 device properties such as retail price, vendor, device age, screen size and more to allow more granular analytics and better content optimization
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Find out how our products have been used to improve user experience and to maximise revenue.
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Browse the full list of properties available in 51Degrees device detection solutions. Find out what values are supported in the Lite, Premium and Enterprise data sets, including full descriptions and advice on how they can be used.
Enter a user-agent and find out the associated device, operating system, and browser.
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Compares the major measures, licencing models and costs associated with 51Degrees, DeviceAtlas and ScientiaMobile's WURFL product.
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Mobile device emulators for Windows based operating systems recommended by 51Degrees.
Mozilla, among others, started to make digital marketers jobs a lot harder in 2019 when they disabled the analytics tools these professionals rely on to understand customer journeys and improve and monitor their websites.
This blog summarizes Mozilla's position and evaluates alternative solutions, before explaining some simple technical tweaks that can be used to bring analytics traffic back to life.
Categories: Analysis, News
Tags: Analytics, Firefox, Mozilla
51Degrees are often asked how our data compares to other Device Intelligence providers. In this blog we highlight the key differences between the data you can retrieve from Google analytics and the granular device data we can provide.
Categories: Device Data
Tags: Analytics, 51Degrees
Tablet device sales have been declining in recent years. In Dec '17, our data showed that 59.83% of our clients' web traffic came from mobile devices, with 16.22% of that coming from tablets. While not a formidable figure compared to smartphones, it still represents a large enough share to show that brands and e-commerce sectors still need to respect that tablet usage is still present and they need granular device data to optimise their online content. In this article, we take a look at the most popular tablets globally and Apple's dominance in the tablet market.
Categories: Analysis, Device Data, Opinion
Tags: Device Detection, Tablet, Analytics, Device Data
Here at 51Degrees we're often asked how our data compares to other Device Intelligence providers. We have carried out extensive testing using 51Degrees data and Google analytics data to provide an understanding. This blog compares our data to the data provided as default by Google analytics and highlights some discrepancies that can be found.
Categories: Analysis
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51Degrees took a look at the first three month of browsing data for the Samsung Galaxy S4, S5 and S6 as well as the HTC One, HTC One (M8) and HTC One (M9). The objective was to see the volume of web browsing that each device took up as a proportion of browsing on all Samsung or HTC devices.
Tags: Samsung, HTC, Analytics
To gain a better understanding of where the tablet market stands today, without the overpowering 'noise' that Apple creates, 51Degrees – a device detection company - has pulled together data from more than 3 billion unique web sessions per month based on a variety of Android tablets, and their percentage of web traffic, on a global and individual country scale (Germany, UK, USA and India.)
As featured on The Wall blog, click here to read the full piece.
Tags: Tablet, Analytics
The battle between iOS and Android is usually played out in the battlefield of the high street, where the two key mobile operating systems compete for sales. The relative performance of each operating system can therefore fluctuate greatly: the month after a new iPhone launch iOS devices tend to do very well and the same is true when a new Android phone is launched or a price drop takes place.
The question we asked ourselves at 51Degrees.com was what does the battle look like out in the real world.
Elements of this article were featured in The Register. You can see the article here.
Tags: Analytics, Android, iOS
The data in this article was featured on Mobile Marketing Magazine. You can read the article here.
51Degrees takes a look at the current state of BlackBerry, here are the vital stats that can help us analyse the possibilities for BlackBerry.
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With the recent announcement that Windows 10 will finally be available for public release on the 29th July 2015 we take a look at the breakdown of the current web traffic generated by all Windows operating systems during May 2015.
Categories: Analysis, Device Data
Tags: Analytics, Microsoft, Windows

subset: commoncrawl


text:
To better understand off-target effects of widely prescribed psychoactive drugs, we performed a comprehensive series of chemogenomic screens using the budding yeast Saccharomyces cerevisiae as a model system. Because the known human targets of these drugs do not exist in yeast, we could employ the yeast gene deletion collections and parallel fitness profiling to explore potential off-target effects in a genome-wide manner. Among 214 tested, documented psychoactive drugs, we identified 81 compounds that inhibited wild-type yeast growth and were thus selected for genome-wide fitness profiling. Many of these drugs had a propensity to affect multiple cellular functions. The sensitivity profiles of half of the analyzed drugs were enriched for core cellular processes such as secretion, protein folding, RNA processing, and chromatin structure. Interestingly, fluoxetine (Prozac) interfered with establishment of cell polarity, cyproheptadine (Periactin) targeted essential genes with chromatin-remodeling roles, while paroxetine (Paxil) interfered with essential RNA metabolism genes, suggesting potential secondary drug targets. We also found that the more recently developed atypical antipsychotic clozapine (Clozaril) had no fewer off-target effects in yeast than the typical antipsychotics haloperidol (Haldol) and pimozide (Orap). Our results suggest that model organism pharmacogenetic studies provide a rational foundation for understanding the off-target effects of clinically important psychoactive agents and suggest a rational means both for devising compound derivatives with fewer side effects and for tailoring drug treatment to individual patient genotypes.
Author Summary
Neuropsychiatric disorders such as depression and psychosis affect one-quarter of all individuals during their lifetime, and despite efforts to improve the selectivity of psychoactive drugs, all are associated with side effects. Drug efficacy and tolerance are known to be linked to an individual's genetic profile, but little is known about the nature of this correlation due, in part, to the current emphasis on screening compounds against targets in vitro. Here we present a comprehensive, genome-wide effort to understand drug effects on the cellular level using an unbiased genome-wide assay to determine the importance of every yeast gene for tolerance to 81 psychoactive drugs. We found that these medications perturbed many evolutionarily conserved genes and cellular pathways, such as those required for vesicle transport, establishment of cell polarity, and chromosome biology. The 500,000 drug–gene measurements obtained in this study increase our understanding of the mechanism of action of psychoactive drugs. Specifically, this study provides a framework to assess the next generation of psychoactive agents and to guide personalized medicine approaches that associate genotype and phenotype.
Citation: Ericson E, Gebbia M, Heisler LE, Wildenhain J, Tyers M, Giaever G, et al. (2008) Off-Target Effects of Psychoactive Drugs Revealed by Genome-Wide Assays in Yeast. PLoS Genet 4(8): e1000151. https://doi.org/10.1371/journal.pgen.1000151
Editor: Michael Snyder, Yale University, United States of America
Received: April 11, 2008; Accepted: July 2, 2008; Published: August 8, 2008
Copyright: © 2008 Ericson et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by grants to CN from NIH and CIHR (MOP-84305), to GG from NIH and CIHR (MOP-81340) and to MT from CIHR and the Howard Hughes Medical Institute. GG and MT are Canadian Research Chairs. EE holds an Ontario Post-Doctoral Fellowship and is supported jointly by the Ministry of Research and Innovation and CIHR (MOP-81340).
Competing interests: The authors have declared that no competing interests exist.
Neuropsychiatric disorders will effect 25% of all individuals at some point in their lives, with devastating social and economic consequences [1]. This constellation of diseases encompasses schizophrenia, depression, age-related memory and cognition decline, and the degeneration of neuromuscular function. Most prescribed psychoactive drugs are thought to primarily target neurotransmission pathways in the central nervous system, and thereby cause changes in perception, mood, consciousness, and behavior. Many of these therapeutics have been developed using in vitro assays and, as such, may have other unknown targets and unanticipated cellular effects in vivo. For example, side effects of antipsychotic drugs include tremors, hypotension, impotence, lethargy, and seizures [2]. In an effort to improve efficacy and to reduce side effects, new generations of drugs have been developed; among these are the so-called atypical antipsychotics such as clozapine. While clozapine is linked to a reduced risk of neuromuscular side effects, it is associated with new side effects such as life-threatening agranulocytosis in up to 1% of patients [3], and, less frequently, fatal myocarditis [4]–[6]. As such, the therapeutic benefit of this and other new atypical drugs remains open to debate. For example, a comprehensive meta-regression analysis that compared both typical and atypical drugs concluded that atypical antipsychotics were neither more effective nor better tolerated than conventional agents [7]. Other classes of psychoactive drugs, such as the antidepressants, also cause numerous undesirable side effects and the broad usage of these medications have been questioned [8].
Surrogate genetics is an effective approach to interrogate heterologous gene function or drug mechanism of action using simpler model organisms [9],[10]. The budding yeast Saccharomyces cerevisae has previously been used to help elucidate the basis of some psychiatric disorders [11]–[18]. For example, the expression in yeast of mutant and wildtype forms of the Huntington's disease gene revealed important factors regulating the toxicity of protein aggregates [11],[15],[19], and a genome-wide suppressor screen in yeast uncovered kynurenine 3-monooxygenase as a potential new therapeutic target for the treatment of Huntington's disease [13]. In other studies, expression in yeast of the alpha-synclein gene associated with Parkinson's disease yielded a network of interacting genes that modulate cellular toxicity [11],[15],[19].
Recently, the genome-wide collection of yeast gene deletion strains has been used to generate genetic profiles of drug sensitivity and resistance [20]–[26]. These profiles have uncovered unexpected mechanisms of action for well-known drugs, such as for the anti-metabolite 5-fluorouracil in perturbation of rRNA processing [22],[25] and for the anti-cancer agent tamoxifen in calcium homeostasis [26].
To better understand potential off-target effects of FDA-approved psychoactive drugs and their analogs, we profiled 214 psychoactive compounds in quantitative wildtype yeast growth assays and generated genome-wide deletion sensitivity profiles for the 81 drugs that caused overt growth defects. The sensitivity profiles for 49 of these drugs were overrepresented for core cellular functions such as chromatin organization, establishment of cell polarity, and membrane organization and biogenesis. Our results provide a rational foundation for personalized drug approaches and for understanding unwanted side effects in clinically important psychoactive agents.
Specific Classes of Psychoactive Drugs Have Bioactivity in Yeast
To ask if psychoactive compounds can inhibit wildtype budding yeast growth, we challenged yeast with 76 high-purity psychoactives representing 16 ligand categories that encompass a broad spectrum of treatments for neurological disorders (see Figure 1 for workflow and Table S1 for drug information). Despite the fact that yeast lacks the established neuronal targets of these compounds, 17/76 (22%) drugs inhibited the growth of wildtype yeast (when tested at 200 µM) and are hereafter referred to as "bioactive". This observation shows that in addition to their reported targets, many of these compounds also have secondary mechanisms of action. In fact, over half of the 16 tested ligand classes included compounds that were bioactive (Figure 2A). Among these, serotonin uptake inhibitors were most effective; four of five tested molecules in this class inhibited yeast growth (Figure 2A). Because our assay depends on growth inhibition in order to observe any effects on specific deletion strains, we proceeded with the 17 bioactive compounds and determined a drug dose that inhibited wildtype growth by ∼15% (Figure 2B, Table S1). In our previous genome-wide studies this level of inhibition best captured the ability to identify the known drug target while minimizing the number of generally sensitive strains [22],[23]. Applying this drug dose, we subjected the bioactive compounds to genome-wide parallel fitness profiling. In this technique, pools of deletion strains are grown competitively for several generations in the presence of a sub-lethal concentration of drug, and genomic DNA is extracted. After PCR-amplification of the unique molecular barcodes incorporated into each gene deletion cassette, the relative role of each gene for growth in the presence of drug is determined by hybridization of the PCR products to a DNA microarray carrying the barcode complements [27]–[29]. The relative abundance of sequence tags in the drug experiments is compared to control experiments and fitness ratios and z-scores are calculated (see Materials and Methods). We used two pools of diploid strains: i) heterozygous deletion strains deleted for one copy of the essential genes (1158 strains), which often identifies compound targets through HaploInsufficiency Profiling (HIP) [22],[30], and ii) homozygous deletion strains deleted for both copies of non-essential genes (4768 strains); this HOmozygous Profiling (HOP) assay identifies genes that buffer the drug target pathway [24].
Schematic overview of the chemical genetic screening process.
(A) Number of drugs that did (black) or did not (white) inhibit wildtype yeast growth for each of the initial drug sets tested. (B) Titration of drug concentrations used in genome-wide screening. Wildtype yeast growth in serial dilutions of drug was recorded as optical density every 15 min over a 25 h period. (C) Number of deletion strains that were sensitive (r>2 and z>3, see Materials and Methods) to bioactive drug in genome-wide fitness profiles.
Using this combination of the HIP and HOP assays we found that only a few deletion strains (∼5) exhibited significant sensitivity to most of the 17 bioactive compounds (Figure 2C). In contrast, several deletion strains (∼50) were scored as sensitive for the α1-adrenoceptor antagonist SR 59230A and the three selective serotonin re-uptake inhibitors fluoxetine (Prozac), clomipramine, and fluvoxamine (Figure 2C). Given this unexpected potency of the serotonergic drugs in our yeast assays, we extended our investigation to encompass pharmacologically related agents and screened two commercially available drug libraries encompassing 95 serotonergic and 55 dopaminergic compounds. These drug libraries contained the four FDA-approved serotonergics sertraline (Zoloft), fluoxetine (Prozac), paroxetine (Paxil), and cyproheptadine (Periactin), and the four FDA-approved dopaminergics bromocriptine (Parlodel), clozapine (Clozaril), haloperidol (Haldol), and pimozide (Orap). Based on our initial results, we anticipated a high rate of bioactivity on yeast for these two drug classes. Indeed, 66/150 (44%) of the serotonergic and dopaminergic drugs were bioactive, a significant difference compared to the 22% of the initially screened drugs that represented the 16 different ligand sets (p<10−7).
Physiochemical Properties Separating Active from Inactive Drugs
The high prevalence of bioactivity in yeast prompted us to ask if any particular psychoactive drug attribute correlated with the ability of these compounds to inhibit wildtype yeast growth. We first performed structural clustering of all ∼220 screened psychoactive compounds using chemical fingerprints in Pipeline Pilot (Accelyrs, San Diego). As more than half of the resulting clusters contained both active and inactive drugs, chemical structure was not predictive of drug action on wildtype yeast growth for this selection of compounds (data not shown). We next asked if any physiochemical properties, as predicted from the structures, were linked to drug activity. The parameters we tested included the number of H-bond donors and acceptors, molecular weight, and hydrophobicity as measured by AlogP (the octanol-water partition coefficient). These measures are important descriptors used in the empirical parameter set known as Lipinski's Rule of Five [31]. In addition to the Lipinski descriptors, we tested six other parameters relevant to drug activity: van der Waals surface area, molecular surface area, molecular solubility, logD (the octanol-water distribution coefficient; a combination of logP and pKa), number of rings and number of rotatable bonds. Principal component analysis revealed that a partition coefficient of AlogP>3 was best able to predict drug activity (p<4.9e-13, for details see Materials and Methods) as shown in Figure 3. A molecular weight of >260g/mole was also indicative of an active compound (p<3.4e-05, Figure 3). If there is a correlation between human side-effects and conserved cellular pathways scored using our surrogate yeast system, it is possible that an additional study could help predict such effects based on structural features.
All compounds tested were plotted as a function of logP and molecular weight.
Genome-Wide Fitness Profiles on Bioactive Drugs
To systematically interrogate compound mechanisms of action, we subjected the 66 bioactive serotonergic and dopaminergic compounds to genome-wide fitness assays using the approach described above (Figure 1). Combined with the initial set of 17 bioactive drugs, we screened a total of 81 unique drugs (two drugs occurred in duplicate in the chemical libraries), eight of which are used therapeutically (Table 1). Fitness ratios and z-scores for all deletion strains are provided in Tables S2 and S3, respectively (raw data are available at ArrayExpress, EMBL-EBI, accession number E-MTAB-34). The genome-wide fitness profiles were reproducible as the average correlation coefficient for the five replicated compounds was 0.83, which is similar to the average correlation coefficient of 0.72 reported in a previous large-scale fitness study [23]. As an unbiased control, we calculated the average correlation coefficient between all possible random drug pairs in our assay. As expected, this value (0.44) was lower than the average correlation coefficient for duplicates, but well above the previously noted average correlation of zero for unrelated compounds (Maureen Hillenmeyer, unpublished data). In agreement with this, two-dimensional hierarchical clustering [32] did not separate the dopaminergic and serotonergic profiles into two distinct groups, but clearly separated drugs from these two classes from most other compounds profiled (Figure 4). Further indicating the general similarities between dopaminergic and serotonergic drugs in our yeast screen, 25% of the significantly sensitive strains (r>2, z>3, see Materials and Methods) scored in both drug categories (Table S4).
Two-dimensional hierarchical clustering [32] was used to group all log2-fitness ratios obtained from the 81 drugs. Log2-fitness ratios from 0 (no fitness defect) to 3.5 (severe phenotype) are color-coded according to the severity of the sensitivity (this paper focuses on sensitivities, see Materials and Methods). Only 0.1% of the log2-fitness ratios were higher than 3.5 and became saturated in the figure. The separation of dopaminergic and serotonergic drugs (orange) from drugs in other categories (grey) is indicated. Groups of strains exhibiting highly similar fitness profiles across the psychoactive drugs are extracted from the global clustergram, and the deletion strains included in each group are listed in the order determined by the hierarchical clustering algorithm. For each group of strains, the dominant function(s) of the deleted genes is indicated. Essential genes are underlined.
Core Cellular Processes that Confer Resistance to Psychoactive Compounds
To ask which cellular functions and pathways were required for resistance to the tested drugs, we performed functional enrichment tests using Gene Ontology (GO) annotations specifically focusing on sensitive strains in the i) essential heterozygous, ii) homozygous or iii) both collections (see Materials and Methods). 32 drug sensitivity profiles were not enriched for any GO Process but the remaining 49 profiled drugs (60.5%) interfered with 106 different processes (multiple-testing corrected p-value<0.0001, Table S5). For visual clarity, we collapsed these 106 processes down to 22 (Table S6). The drug sensitivity profiles obtained with the combined set of heterozygous and homozygous strains were enriched for the highest number of condensed GO processes (119 processes, purple color in Figure 5), while 12 processes were uniquely enriched among sensitive homozygous deletion strains (blue color in Figure 5). These processes likely reflect drug detoxification mechanisms (e.g. "vesicle transport" and "response to drug") or other processes required for resistance to compound by an unknown mechanism (e.g. "amino acid biosynthesis and metabolism"). Two processes were uniquely scored for essential genes (red color in Figure 5) and are further discussed below.
Significant enrichment (p<0.0001) for GO Processes uniquely scored using sensitive (z>2) homozygous strains (blue) or heterozygous strains deleted for essential genes (red). GO Processes scored using both strain pools are indicated in purple (for details see Materials and Methods). Dopaminergic drugs are indicated in green, serotonergic in orange, and other drugs (from the initially analyzed diverse set) in grey. Drugs with affinity for both a dopaminergic and a serotonergic receptor are indicated according to which Tocris drug library they belong to. Closely related GO categories are collapsed for clarity (see Table S6).
Investigating the general nature of our enrichment profiles, we found that the most frequently enriched processes across all drugs and genetic backgrounds were vesicle transport, protein localization, and telomere biology (Figure 5). Genes functioning in cell morphogenesis, establishment of cell polarity, cell cycle, amino acid biosynthesis, chromatin organization, RNA metabolism, and membrane organization were also needed for resistance to several (>5) of the psychoactive drugs. A few GO Processes were unique to a single drug: protein glycosylation (A77636), methylation (SB 216641), cell wall organization and biogenesis (GR 127935), and membrane lipid metabolic process (pimozide). In the subsequent sections we focus on the analysis of the FDA-approved drugs and summarize the most notable enrichments for these drugs in Table 2. First, we discuss identified buffering pathways and drug detoxification mechanisms. Next, we concentrate on potential new drug targets identified for the therapeutically used psychoactive drugs.
Uncompromised Cellular Transport, Protein Localization, and Telomere Maintenance Is Important for Resistance to Psychoactive Drugs
Vesicle transport was the most commonly overrepresented process among genes required for resistance to psychoactive drugs (Figure 5) suggesting that uncompromised vesicle transport function is a general requirement for psychoactive drug detoxification. The enrichment of cellular transport genes was especially pronounced in response to clozapine treatment, where 9 of the 10 most required genes belonged to this category (Table 3). Protein sorting and localization accounted for the second most frequently enriched process (Figure 5). Deletion of vesicle trafficking and protein localization genes often resulted in very severe phenotypes (bright yellow in Figure 4). Gene products with protein localization roles include those involved in selecting cargo proteins for endosome-to-Golgi retrieval (e.g. Vps29), and those involved in sorting proteins in the vacuole (e.g. Pep8). Interestingly, the fitness profiles obtained with certain vesicle transport and protein localization deletions clustered with those obtained with strains deleted for genes functioning in actin filament organization/stabilization (arc18Δ, tpm1Δ, vrp1Δ,), mRNA degradation (lsm1Δ), and stabilization of membrane amino acid transporters (npr1Δ) (Figure 4, left text panel). A second, large group of strains mainly deleted for genes functioning in vesicle transport and protein localization exhibited similar phenotypes across the 81 drugs as ckb1Δ and ckb2Δ, which are deleted for genes functioning in regulation of transcription and mitotic cell cycle (Figure 4, right panel).
Most of the drug sensitivity profiles were enriched for both protein localization and telomere biology (Figure 5). The apparent "linking" of these enrichments could be attributed to genes that are, in fact, involved in both these processes. Examples of such genes function in the three Endosomal Sorting Complexes Required for Transport, more specifically in ESCRT I (VPS28, STP22), in ESCRT II (SNF8 and VPS25), and in ESCRT III (SNF7). These genes are, in addition, associated with telomere defects [33],[34].
The Atypical Antipsychotic Clozapine Has No Fewer Off-Target Effects than Typical Antipsychotics
Because the more recently developed atypical antipsychotic drugs are still associated with side effects and their benefits are currently debated, we compared the phenotypic profiles of the atypical antipsychotic clozapine to two traditional antipsychotics, reasoning that if atypical drugs are more specific, they would exhibit fewer off-target effects in yeast. In contrast to this expectation, the atypical antipsychotic clozapine exhibited a similar number of significantly sensitive (r>2, z>3, see Materials and Methods) deletion strains (26) as the typical antipsychotic drugs pimozide (29) and haloperidol (20). Comparing the fitness profiles of clozapine with the typical antipsychotics pimozide and haloperidol, we found that each drug was associated with unique functional enrichment profiles: clozapine for telomere biology and protein localization, pimozide for membrane lipid metabolic processes, and haloperidol for aromatic amino acid biosynthesis and metabolism (Figure 5). In contrast, vesicle transport was enriched in all three drug sensitivity profiles. The more detailed GO processes behind the condensed process vesicle transport were vesicle-mediated transport for all three drugs and, in addition, secretory pathway, secretion, post-Golgi vesicle-mediated transport and Golgi vesicle transport for haloperidol and clozapine (Tables S5 and S6). The distinct fitness profiles are consistent with the structural differences that exist between these drugs (Figure S1). For example, clozapine has substructures (piperazine and diazepine) that do not exist in pimozide and haloperidol, and haloperidol contains two benzene rings while pimozide has three.
Bromocriptine, Haloperidol, and Five Additional Dopaminergic Drugs Interfere with Amino Acid Biosynthesis and Metabolism
Compared to the other investigated therapeutics, the fitness profile in the anti-Parkinson drug bromocriptine pointed to a single potential off-target mechanism of action for this drug. The only overrepresented function among sensitive strains was amino acid biosynthesis and metabolism (Figure 5) and the most sensitive strains were deleted for the aromatic biosynthesis genes TRP3, TRP4, TRP1, ARO1, TRP2, and ARO2. In addition to bromocriptine, six other dopaminergic drugs also interfered with amino acid biosynthesis and metabolism (Figure 5). The sensitivity profiles of all these seven drugs shared the enrichment for the detailed GO process aromatic compound metabolic process (Tables S5 and S6) due to the sensitive phenotype of 13 strains in total. Among them, strains deleted for TRP1, TRP2, TRP3, TRP4, TRP5, ARO2, and ARO3 were scored in all 7 drugs and strains deleted for ARO1 and ARO7 in 6 drugs. Besides the notable enrichment for genes involved in aromatic compound metabolism, the sensitivity of strains missing other genes also contributed to the observed GO process enrichment. Such genes included the folic acid (vitamin B9) biosynthesis gene FOL2, the panthothenate (vitamin B5, precursor of coenzyme A) biosynthesis gene FMS1, and the protein kinase GCN2, which induces amino acid biosynthesis genes in yeast in response to starvation and, in addition, restricts intake of diet lacking essential amino acids in rats [35].
Pimozide Is Unique in Being Enriched for Membrane Lipid Metabolic Process Genes
The sensitivity profile of the typical antipsychotic pimozide showed a unique enrichment for membrane lipid metabolic processes not seen for any of the other 80 profiled drugs (Figure 5). In pimozide, the MCD4-deletion strain had the strongest phenotype and was 21-fold depleted compared to the control (Table 3). MCD4 is highly conserved among eukaryotes and functions in glycosyl-phosphatidylinositol (GPI) anchor synthesis. Because MCD4 is an essential gene, it may represent an additional, clinically relevant drug target for pimozide. The inositol-lipid-mediated signaling gene PIK1 and the spingholipid-mediated signaling gene YPK1 were also among the ten most required genes for resistance to pimozide (Table 3). They clustered with a group of other strains deleted for genes involved in lipid biology (Figure 4), such as the de novo lipid synthesis genes PAH1 and SUR4.
Fluoxetine Interferes with the Establishment of Cell Polarity
Eight drugs, among them the antidepressant fluoxetine, were enriched for the condensed term establishment of cell polarity (purple or blue color in Figure 5). In total, 51 genes were assigned to the detailed GO process establishment and/or maintenance of cell polarity and caused a sensitive phenotype when deleted (Tables S5 and S6). Many of these genes scored in the majority of the drugs, for example all four members (CKA1, CKA2, CKB1, and CKB2) of the casein kinase II-holoenzyme complex, and TPM1, the major isoform of tropomyosin which directs polarized cell growth and organelle distribution. For the seven drugs where the enrichment for establishment and/or maintenance of cell polarity was scored using sensitive homozygous and essential heterozygous strains (purple color in Figure 5), six essential members (EXO70, SEC3, SEC6, SEC8, SEC10 and SEC15) of the exocyst complex, which determines where secretory vesicles dock and fuse, were scored in all drugs except fluoxetine.
Essential Genes of High Importance for Drug Resistance May Reveal Additional Secondary Drug Targets for Psychoactive Drugs
Drug targets are often encoded by essential genes, thus essential genes scored in our assay may represent important additional targets of psychoactive compounds that may be useful in the development of therapeutics for other applications. In a given heterozygous strain, the reduced gene copy number of a potential drug target leads to a reduced level of the corresponding protein. When this strain is grown in the presence of a drug targeting the heterozygous locus, the result is a further decrease in "functional" dosage due to the drug binding to the protein target. If this protein is important for growth, the result will be drug sensitivity [22]. In our functional enrichment tests, two processes were uniquely overrepresented among sensitive essential genes (red color in Figure 5): mitotic and meiotic cell cycle for fluorophenyl-methoxytropane and chromatin organization for cyproheptadine. Examples of targeted essential genes in cyproheptadine treatment include chromatin-remodeling genes (ARP4, ARP7, ARP9), genes in the multisubunit (NuA4) histone acetyltransferase complex (EPL1, ESA1, SWC4), and RSC4 and RSC6 in the RSC Chromatin remodeling complex.
Although not revealed as a functional enrichment among sensitive strains deleted for essential genes, most of the other FDA-approved drugs also have potential secondary drug targets as infered by the presence of essential genes among the ten most required genes for drug resistance (Table 3). As judged by the high number of sensitive strains deleted for essential genes in paroxetine treatment (10 strains) and sertraline treatment (9 strains), these selective serotonin re-uptake inhibitors are particularly rich in potential secondary drug targets. Essential genes required for resistance to the FDA-approved drugs include those involved in RNA processing, transcription and translation, genes functioning in the protein folding chaperonin complex, and the chromatin-remodeling/DNA repair gene ARP4 (bold in Table 3). Deletion of ARP4 resulted in some of the most sensitive phenotypes when cells were treated with cyproheptadine, sertraline, or with haloperidol (Table 3). ARP4 has a close human homolog, ACTL6B, which encodes a subunit of the BAF (BRG1/brm-associated factor) complex in mammals, functionally related to the SWI/SNF complex in S. cerevisiae. The SWI/SNF complex is thought to facilitate transcriptional activation by antagonizing chromatin-mediated transcriptional repression [36]. Another example of an essential gene required for drug resistance in several FDA-approved drugs is GSP1, which functions in RNA-processing (Table 3). The mammalian homolog of Gsp1, Ran (BlastP E-value<E-261) is, as in yeast, a nuclear GTP-binding protein.
Interestingly, the fitness profile of the ARP4-deleted strain was very similar to the strains deleted for the cytosolic chaperonin subunits CCT5, CCT8 and TCP1 (Figure 4). The chaperonin complex is involved in protein folding (primarily of actin and tubulin) and cytoskeleton organization [37]. In our fitness assays, seven of eight CCT-strains scored as significantly sensitive in many of the probed psychoactive drugs. Some (CCT3, CCT4, CCT7 and CCT8) were even among the top-ten required genes for resistance to cyproheptadine, fluoxetine, paroxetine, and sertraline (Table 3). Furthermore, several deletion strains with uncharacterized functions had similar fitness profiles as the chaperonins CCT5, CCT8 and TCP1 (Figure 4). Among them were TVP23 and YIP5 which both localize to the late Golgi, YEL048C which is synthetic lethal with GCS1 (involved in ER to Golgi transport), APM2 (homologous to medium chain of mammalian clathrin-associated protein complex involved in vesicle transport) and SWH1 (similar to mammalian oxysterol-binding protein, localized to Golgi and nucleus-vacuole junction).
Psychoactive Drugs often Impinge on Evolutionarily Conserved Processes
To test if our findings in yeast might reflect drug action in human cells, we looked at the proportion of scored genes with human homologs. Among the strains significantly sensitive to at least one psychoactive compound, 58.4% were deleted for a gene with a close human homolog (BlastP E-value<E-6), as compared to 45.0% for all analyzed deletion mutants regardless of whether they had a fitness defect or not. To test if strains deleted for genes involved in core cellular processes are more sensitive in general, we compared our results obtained with the 81 psychoactive compounds to 81 randomly chosen chemically diverse compounds (see Materials and Methods). We found that a similar proportion of genes with close human homologs (59.7%) were scored for strains that were significantly sensitive to at least one of these diverse chemicals. Despite this similarity in proportion of sensitive strains with human homologs in the two datasets, conserved genes were scored much more frequently (in >10% of the compounds) in the psychoactive drug set than in the random drug set. In fact, considering only genes deleted in frequently scored strains, 64.1% of the psychoactive drugs had close human homologs (BlastP E-value<E-6) while the corresponding proportion for the structurally diverse drug set was significantly (p<0.006) lower (45.4%) and similar as the fraction of human homologs for multi-drug resistance genes (47.1%) in a recently published study [23]. This difference points to a significant enrichment of frequently scored sensitive strains with human homologs for the psychoactive drugs. Among the strains sensitive to the highest number of psychoactive compounds, seven of eight had close human homologs: NEO1, SAC1, PIK1, VPS29, PEP8, ARP4 and VPS35. The majority of these genes are involved in vesicle transport, which was the most frequently enriched function among strains sensitive to psychoactive drugs. Thus, the specific psychoactive drug detoxification mechanisms identified in yeast are likely to be of importance in humans treated with psychoactives.
Many psychoactive drugs are associated with adverse secondary effects in humans yet the mechanisms that underlie these off-target effects are poorly understood. To address mechanisms of drug action in a systematic manner, we profiled the genome-wide collection of budding yeast deletion strains for sensitivity to a broad spectrum of psychoactive compounds, of which dopaminergic and serotonergic drugs had a high bioactivity. Among 214 tested compounds, we uncovered 81 drugs that conferred a measurable growth defect on wildtype yeast. An appropriate dose of these active compounds was applied to the pooled heterozygous and homozygous yeast deletion sets to identify genes whose function is required for optimal growth in the presence of drug. Fifteen percent of all yeast strains (deleted for non-dubious ORFs) exhibited significant sensitivity (r>2, z>3) to these 81 psychoactive compounds and more than half of the drugs interacted with core cellular functions. Several clinically important drugs, such as fluoxetine, cyproheptadine, and clozapine were linked to diverse cellular processes. This observation may explain both the diversity of side effects observed in human patients and the therapeutic variability associated with these drugs. That is, polymorphisms in any of the conserved processes affected by a given drug are a likely source of the individual variation in response to drug. For instance, the response to the frequently prescribed antipsychotic clozapine is highly variable between individuals as the same dose can have markedly different efficacy and/or side effects in different patients [38]. Genes functioning in vesicle transport, protein localization, telomere biology, and catabolic processes were required for clozapine resistance in yeast. In another example, fluoxetine is associated with side effects such as seizures, nausea, sleepiness, anxiety, and serious allergic reactions. This antidepressant affects numerous cellular processes including establishment of cell polarity, protein localization, and cytoskeleton organization and biogenesis. Given the limited number of FDA-approved drugs within the set of 81 compounds analyzed here and the overlapping side effects associated with these drugs, it is not yet possible to correlate any single side effect to a particular perturbed pathway.
The most frequently scored sensitivity for the 81 profiled antipsychotic drugs was due to loss of secretory pathway function, likely indicating the importance of vesicle transport (e.g. to the vacuole) for drug detoxification. The lysosome (the mammalian vacuole equivalent) is known as the major site of degradation of both exogenous and endogenous molecules. For FDA-approved drugs, the requirement for vesicle transport genes was reflected in the frequent sensitivity of the neo1 deletion strain as the most sensitive strain in six FDA-approved drugs. Neo1 is an essential, highly conserved type 4 P-type ATPase involved in intracellular membrane- and protein-trafficking. Members of this family of P-type ATPases are implicated in the translocation of phospholipids from the outer to the inner leaflet of membrane bilayers. Our data suggested that interference with membrane structure and transport through inhibition of Neo1 is an additional, unwanted mechanism of action for clozapine, cyproheptadine, fluoxetine, paroxetine, sertraline and haloperidol, and their drug analogs. The importance in humans of functional 4 P-type ATPases is well documented as hereditary cholestasis, caused by defects in biliary epithelial transporters, has been directly linked to mutations in a 4 P-type ATPase gene [39].
In addition to the frequently observed requirement for uncompromised vesicle transport for drug detoxification, several drug sensitivity profiles were enriched for more specific processes. Within the FDA-approved drug group, the antidepressant paroxetine was unique in targeting RNA processing genes, pimozide interfered with membrane lipid metabolic processes, cyproheptadine preferentially targeted essential genes with chromatin remodelling functions, and fluoxetine interfered with establishment of cell polarity. Furthermore, seven dopaminergic compounds including the anti-Parkinson drug bromocriptine resulted in sensitivity of strains deleted in aromatic amino acid biosynthetic genes. This sensitivity may be a result of that dopaminergic drugs block aromatic amino acid uptake in yeast, requiring yeast to activate the corresponding biosynthetic pathways. Given the fact that aromatic amino acids are precursors to dopamine and serotonin, this was an interesting observation suggesting that the levels of intracellular precursors may be important in the response to certain psychoactive drugs.
Interestingly, interference with members of the chaperonin complex resulted in some of the most severe phenotypes. Seven of eight CCT-strains scored as significantly sensitive in several psychoactive drugs, among them CCT5. The human homolog of this gene is associated with hereditary neuropathy [40]. Although it is unclear how mutated CCT5 causes this disease, it has been postulated that its mutation leads to accumulation of misfolded cytoskeletal proteins, leading to defective assembly of actin into microfilaments resulting in neuronal apoptosis [41]. In our yeast screens, CCT5 was needed for resistance to eight different compounds (cyproheptadine, paroxetine, fluoxetine, indatraline, MDL72222, CY208-243, 2-Chloro-11-(4-methylpiperazino)-dibenz[b,f]oxepin, N-Desmethyl-clozapine, and 3-alpha-[(4-Chlorophenyl)-phenylmethoxy]-tropane. We conclude that interference with tubulin and actin folding is an important, secondary mechanism of action of these compounds.
As an example of how the information from our yeast assays may lead to testable drug-gene interaction hypotheses in humans, we found that the levels of the yeast strain heterozygous for ACC1 was eleven-fold reduced in ritanserin as compared to the control, indicating that the acetyl-CoA carboxylase Acc1 may be a secondary target of ritanserin. Like its yeast counterpart, the human homolog ACACA is required for de novo biosynthesis of long-chain fatty acids and its activity drops during fasting [42]. Because increased appetite is a reported side-effect during ritanserin treatment [43], it is tempting to speculate that biochemically mimicking fasting would increase appetite.
These studies raise several important issues for further consideration. Understanding the mechanisms that underlie adverse effects of clinically approved drugs is crucial for the development of next generation therapeutics with improved selectivity and efficacy. Moreover, knowledge of patient polymorphisms in off-target pathways may allow adverse effects of any given drug to be preempted by personalized pharmacogenomic strategies. It is also conceivable that some of the observed secondary drug effects are critical for therapeutic benefit.
In summary, a number of cellular processes were associated with sensitivity to the dopaminergic and serotonergic classes of psychoactive compounds. This points to additional, previously uncharacterized mechanisms of action for these drugs in humans and suggests follow-up experiments aimed at understanding a drug's mechanism of action on a genome-wide level. Our results suggest that model organism pharmacogenetics can be used as a comprehensive and unbiased tool in initial studies aiming at unraveling secondary effects and mechanisms of action for therapeutic compounds and their analogs. A more rigorous understanding of the complete mechanism of drug action in humans would be beneficial in the development of a new generation of better tolerated psychoactive drugs, and in personalized medicine.
Materials and Methods
Compound Libraries
High purity compounds for genome-wide fitness profiles were obtained from Tocris BioScience (http://www.tocris.com) as ligand sets and as the serotonergic (#1732) and dopaminergic (#1718) collections. In total, these drug collections comprised 226 drugs, 12 of which overlapped between the collections. A complete list of drugs, catalogue numbers, solvents, and concentration used in the genome-wide screens is provided in Table S1.
Genome-Wide Yeast Growth Assay
For genome-wide fitness profiles, the complete sets of ∼4700 homozygous deletion strains and ∼1100 essential heterozygous deletion strains in the BY4743 and BY4744 backgrounds (MATa/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 lys2Δ0/LYS2 MET15/met15Δ0 ura3Δ0 /ura3Δ0 ORF::kanMX4) were used [29],[44]. A strain in the same genetic background with YDL227C replaced by kanMX4 was used as the wildtype control for drug titration curves. Strains were stored in 7% DMSO at -80°C. Because all experiments were performed in rich media (YPD [45], without antibiotics), it is unlikely that the presence of auxotrophies had a major effect on our results, however, we cannot rule out that the disruption of the corresponding pathways in yeast may, in some cases, alter our findings. Beginning from an initial maximal concentration of 200 µM, the degree of growth inhibition was determined by exposing wildtype cells to a serial dilution of compound until only a slight inhibition (∼15%) of wildtype growth was observed (see Figure S1). Cells were inoculated at an OD600 of 0.0625 in serial dilutions of drug and grown in a Tecan GENios microplate reader (Tecan Systems Inc., San Jose, USA) at 30°C with orbital shaking. Optical density measurements (OD600) were taken every 15 minutes until the cultures were saturated, and doubling time (D) was calculated as described [46]. Fitness assays using pooled deletion strains were performed as described [47] with the following modifications: i) after growth, 350 µl from each of two independent cultures of the 5-generation homozygous pool and 350 µl from the 20-generation heterozygous essential pool were combined, thereby allowing for approximately equal representation of barcodes for PCR reactions and hybridization to the same DNA chip using the unique barcodes incorporated in each of these strains. ii) for amplification of the tags, ∼0.2 µg genomic DNA was combined with a 1 µM mix of either up- or down-tags and 82% (v/v) Platinum High Fidelity PCR Supermix (Invitrogen # 11306-016) containing anti-Taq DNA polymerase antibody, Pyrococcus species GB-D thermostable polymerase, recombinant Taq DNA polymerase, Mg2+, and dNTPs, iii) extension temperature was 68°, iv) extension was for 2 min except for a final 10 min extension v) 34 cycles of amplification were performed, vi) after 10-16 h, the hybridization mix was removed from Affymetrix Gene Chips, replaced with Wash A (6x SSPE, 0.01% Tween), and chips were stained and washed using GeneChip Fluidics Station 450 (Affymetrix) according to the GeneFlex_Sv3_450 protocol with one additional wash A cycle before the staining.
Data Analysis
Intensity values for the probes on the chip were extracted using the GeneChip Operating Software (Affymetrix). Quantile normalization, outlier omission, fitness defect ratio (denoted as "r") and z-score (denoted as "z") calculations were performed as previously described [47],[48]. In short, fitness defect ratios were calculated for each deletion strain as the log2 of the ratio between the mean signal intensities of the control and the drug chips. The larger the ratio, the more depleted (sensitive) is the strain as compared to control condition without the drug. To include the variance in the control experiments, we also calculated z-scores for each gene by dividing the difference in mean intensity across the control chips and treatment with the mean standard deviation of the signal intensities for the given gene across all 18 control chips [48]. The larger the z-score, the more likely it is that a given strain is significantly depleted from the pool. In our analysis, we scored a deletion strain as significantly sensitive using a threshold for both z-score and log2 intensity ratio. A threshold of z>3 was selected based on our earlier observations that above this limit, 100% of 186 deletion strains detected as sensitive by microarray could be confirmed using individual growth assays [24]. This stringent threshold was chosen to minimize the number of false positives. In addition, we added a further requirement that a sensitive strain should display at least a fourfold depletion (r>2, i.e. log2>2) compared to the control condition. This criterion was added to avoid including z-scores which were artificially high due to a low standard deviation in the control chips. Due to the way the screens were performed (at low drug concentration, i.e. an IC15) and analyzed [22],[24] we have focused on sensitive strains in this work, as opposed to apparently resistant strains. Two-dimensional hierarchical clustering of the fitness ratios was performed using Pearson correlation [32] and the data was visualised using the MultiExperiment Viewer from the TM4 microarray software suite (http://www.tm4.org/index.html).
In each of the 81 profiled drugs, sensitive deletion strains were tested for Gene Ontology Functional enrichment using the standard hypergeometric test provided by the GoStats Bioconductor modules for R [49]. For each drug, we performed three independent functional enrichment tests using i) sensitive heterozygous strains deleted for essential genes (z>2), ii) sensitive homozygous strains (z>2), and iii) all sensitive strains in the given drug with z>2. As the global control set, we used all yeast ORFs in the corresponding deletion background with chip intensity values above background. The background was determined as the average value of all unused tags on the chip (∼3600 tags×5 copies = 18000 values) +2 standard deviations of the background tags. Obtained p-values were corrected for multiple testing by multiplying by the number of identified terms. Adjusted p-values<0.0001 were considered significant. GO processes linked to less than 20 or more than 300 genes in our background set were excluded from our tests. Two-dimensional hierarchical clustering of overrepresented GO processes was performed using binary data [50]. To test the robustness of our functional enrichment tests, we repeated the same analysis using each of the following thresholds: z>3, r>2, r>3 and found consistent functional enrichment profiles.
In the calculations of the proportion sensitive strains deleted for genes with close human homologs (Blastp E-value<E-6), we used a set of 81 recently profiled (our unpublished data) compounds with potency against wildtype yeast. These compounds represent structurally diverse chemicals derived from Chemical Diversity Labs, Inc. repository of >500,000 compounds.
Structure data files were obtained from Tocris and Pubchem for all compounds and Babel canonical smile strings were generated. In the chemical structure clustering, extended connectivity fingerprints based on functional classes in Pipeline Pilot were used [51]. In the physiochemical property clustering, ten descriptors representing important properties for potential drug candidates were calculated after salts were stripped, using Frowns and Openeye cheminformatic libraries [31],[52]. PCA was used to find the strongest properties that separated active from non-active compounds. The revealed properties ALogP and molecular weight were validated to see how they correlated with the pattern of the other eight descriptor loadings. The non parametric Wilcoxon rank sum test supported LogP (p-value 4.91e-13) and MW (p-value 3.42e-05) as significant representative properties.
All supplementary data can also be downloaded from our webpage, http://chemogenomics.med.utoronto.ca/Supplemental/psychoactives/.
Supporting Information
Figure S1.
Chemical structures of the atypical antipsychotic clozapine and the typical antipsychotics haloperidol and pimozide.
Table S1.
Drugs used in genome-wide fitness profiles. Catalogue number (Tocris), solvent, highest drug concentration tested in wildtype yeast, drug concentration used, and brand names for FDA-approved drugs.
Table S2.
Fitness ratios for indicated compounds for all deletion strains with intensity values above background.
Table S3.
Z-scores for the indicated compounds for all deletion strains with intensity values above background.
Table S4.
Strains scored as significantly sensitive with at least one dopaminergic or one serotonergic drug. For each strain, the number of dopaminergic and serotonergic drugs that caused significant depletion in the pool is shown (r>2, z>3). Strains scored in both drug classes are indicated with "1".
Table S5.
Significantly enriched (p<0.0001) GO Processes in genome-wide fitness profiles.
Table S6.
Sub-grouping of the enriched GO categories.
Author Contributions
Conceived and designed the experiments: EE GG CN. Performed the experiments: EE MG. Analyzed the data: EE LEH JW. Contributed reagents/materials/analysis tools: LEH GG. Wrote the paper: EE MT GG CN.
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I'm thinking about getting the Nikon D7000 body-only, and want to know how to tell an FX lens from a DX lens. I may upgrade in the future to an FX body and would like to have glass ready to go when I do.
I was looking on the B&H site and can't tell what lenses are FX and what's DX. Any help on this?


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Email our sales team, or call them on +44 117 990 2960
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The Village of San Fortunato
The village of San Fortunato belongs to the municipality of Rimini, in the province of Rimini, region Emilia-Romagna.
>>>COVID-19<<< Data about spreading of Covid-19 in San Fortunato are not yet available.
For the province of Rimini, to whom San Fortunato belongs, on 2020-05-31 are reported 2,154 cases. In the previos day there were 2,154 cases, thus having a variation of 0 cases, i.e. 0.00% with reference to the number of cases reported in the previous day.
Please follow this link for updated information on Covid-19 including new cases and percentage changes for the whole of Italy, regions and individual provinces
The village of San Fortunato is 4,26 kilometers far from the same town of Rimini to whom it belongs.
To the municipality of Rimini also belong the localities of Bellariva (5,81 km), Belvedere (6,14 km), Borgo Nuovo (11,32 km), Ca' Acquabuona (4,15 km), Ca' Guda (4,06 km), Ca' Palloni (4,21 km), Ca' Rinaldi (4,45 km), Ca' Spina (9,55 km), Ca' Tentoni (11,82 km), Ca' Tomba (7,58 km), Calastra Nuova (5,56 km), Calorè (9,11 km), Casalecchio (7,98 km), Case della Fossa (6,14 km), Case Monte Cieco (10,32 km), Case Orsoleto (5,41 km), Case Pradese (5,51 km), Case sparse (-- km), Casetti (5,80 km), Casetti Prazzolo (3,81 km), Corpolò (12,71 km), Dogana (11,86 km), Fienili (8,98 km), Fornace (10,83 km), Fornace di Miramare (8,56 km), Fornace Marchesini (7,83 km), Gaiofana (7,19 km), Gaiofana DI Vergiano (7,11 km), Ghetto Casale (5,91 km), Ghetto Masere (6,79 km), Ghetto Mavos (4,58 km), Ghetto Petini (4,47 km), Ghetto Piccinelli (5,17 km), Ghetto Randuzzi (6,10 km), Ghetto Tamagnino (8,45 km), Ghetto Tombanuova (8,39 km), Grillo (7,33 km), Il Palazzone (5,56 km), La Brusada (5,72 km), La Cerbaiola (9,20 km), La Fasolina (4,95 km), La Zingarina (8,28 km), Lagone (7,94 km), Le Casette (9,17 km), Macanno (5,80 km), Malte (5,47 km), Marano (9,66 km), Miramare di Rimini (8,59 km), Missiroli (7,23 km), Monte Cieco (10,36 km), Morri (10,52 km), Mulino Carlotti (4,30 km), Orsoleto (6,16 km), Osteria (10,33 km), Osteria del Bagno (7,15 km), Osteria del Fiume (9,55 km), Pelito (7,61 km), Pozzi (8,67 km), Rivabella (0,77 km), Rivazzurra (7,09 km), Sabanelli (4,70 km), San Giuliano (0,81 km), San Giuliano a Mare (1,26 km), San Lorenzo In Correggiano (7,46 km), San Martino In Riparotta (4,86 km), San Martino in Venti (8,69 km), San Martino Monte L'Abate (5,76 km), San Paolo (12,02 km), San Salvatore (9,31 km), San Vito (11,17 km), Santa Aquilina (6,10 km), Santa Cristina (13,65 km), Santa Giustina (8,06 km), Santa Maria in Cerreto (8,06 km), Spadarolo (5,38 km), Stazione Vergiano (6,71 km), Torre Pedrera (6,53 km), Tramontana (10,24 km), Urbinità (9,14 km), Vergiano (6,60 km), Villa Francolini (5,12 km), Viserba (2,58 km), Viserba Monte (2,10 km), Viserbella (4,19 km).
The number in parentheses following each village name indicate the distance between the same village and the municipality of Rimini.
Please notice that in what follows no statistic nor population data are available at the moment: the same are available for the Italian places having al least the status of municipalities ad for most of the villages too.
The distribution of the most commun family names is equally unavailable.
Please find in what follows:
- The banks in San Fortunato, when available.
- The parishes in San Fortunato, when available.
- The pharmacies in San Fortunato, when available.
- Weather and weather forecast for the municipality of Rimini, to whom San Fortunato belongs.
- Hotels in San Fortunato and its surroundings.
- Free ads in San Fortunato and in its province and region too.
- Map and road map of San Fortunato.
- A selection of videos about San Fortunato.
In the hamlet of San Fortunato there is no bank.
Please look at the closest banks in the town of Rimini, to whom San Fortunato belongs.
There is no pharmacy in the hamlet of San Fortunato.
Please look at the closest pharmacies in the town of Rimini, to whom San Fortunato belongs.
The parishes in San Fortunato
There is just a single parish in San Fortunato. The same is listed in what follows, along with some related information.
|Zip Code
|S. Fortunato
|Via Covignano, 257 - San Fortunato
|San Fortunato
|Diocese of Rimini
Where to stay in San Fortunato and its neighborhoods
Hotels, Resorts, B&B in San Fortunato and its neighborhoods.
You can arrange the results by number of stars, by popularity, by distance or even by the guest review score choosing among young couples, mature couples, families with young children, families with older children, people withfriends, or solo travellers.
Free ads in San Fortunato
Most recent free ads in San Fortunato, municipality of Rimini, province of Rimini, Emilia-Romagna region, updated at 19:16:49, 01 June 2020. Place your free ad on San Fortunato: no registration needed. Place your free ad.
Up to now there is no free ad placed in San Fortunato
|Most recent free ads in the province of Rimini
|Rimpatriata con CRISTINA e la IV B 1979 - Forlì
|Affitto stagionale per vacanze a Riccione
|Tranquillo ed ampio trilocale, a due passi dal mare
|Most recent free ads in the region Emilia-Romagna
|Rovinaglia casa affitto annuale
|investire nel rame
Map and road map of San Fortunato
Look at the interactive map of San Fortunato and get the road maps, the satellite maps and the mixed ones too. Interactive maps of San Fortunato also allow an immediate view of: bar,restaurants,pubs,pizzerias,dancing,night clubs,banks,jewellers,beauty centers,hair stylists,supermarkets,pharmacies.
Just fill in the free text search box placed on top of the map with any of previous categories and then press enter.
Please feel free to use the same free text search box placed on top of the map to find any kind of business activities you're interested into.
Videos about San Fortunato Rimini, Rimini, Emilia-Romagna
Several videos about San Fortunato, Rimini, Rimini, Emilia-Romagna.
A first video is shown: clicking on the PLAY button the video will start.
Inside the video window there are several commands and a Playlist button: .
Press the Playlist button to access the complete listing of the available videos, along with their titles. It will suffice to scroll through the list using the cursor to select any of the videos.
Once the video started you can use the and buttons to scroll through the videos. Each video can be displayed in full screen.


text: The Atlantic creole fish has an elongated body with a very short head and a forked tail. This fish can be any color from gray to a very bright red. It inhabits coral reefs and hard bottoms, preferring the shallow water but is found as deep as 165 feet. The creole fish feeds in large groups, often very close to the surface of the water. However, this species tends to hide immediately when alarmed. Did You Know? The creole fish actually varies in color from very bright red to gray. Diet The Atlantic creole fish feeds primarily on zooplankton. Size This fish can reach up to 11 inches in length. Range The Atlantic creole fish is found in the Western Atlantic from the southern United States to Brazil, and the Eastern Atlantic near the islands of Principe and São Tomé. Population Status This species is not threatened. Predators Larger fish and sharks. Back to the Top


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Alberto Juarez, Elena Roche and Quim Diaz have spent many years dedicating their time to scrapbooking and paper crafting and inspiring thousands of people. Their love for crafts has led them to teach hundreds of workshops and design product lines for scrapbooking, such as paper, stamps and paint. They have been part of Sizzix's design team for years. The three are now very happy that their concept of dies is carried out in this new line for journaling.


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A Devastating Injury for the Yanks!…
The injuries have mounted for many teams this spring. We're concerned about Joe Mauer in Minnesota (well, some of us more than others…LOL!) and Cole Hamels in Philadelphia. Justin Duchscherer of the A's will start the season the DL after surgery on his elbow. There are too many injuries, both big and small, to count…
A review of current headlines show that the Braves' Brian McCann is still hurting from getting hit in the ring finger by a foul pitch in yesterday's loss to the Yankees…
…to Angels pitcher John Lackey's stiff forearm.
We've seen Jason Johnson in Yankees camp be diagnosed with eye cancer in the Yankees camp, and of course both Damaso Marte and Robinson Cano came back from the WBC hurting.
But is the biggest injury in Yankees camp the hip surgery performed on A-Rod's torn labrum earlier this month?
No, the most devastating injury in Yankees camp is…
…Derek Jeters's pinkie!
Stop the presses!
Call the ambulance…quick! Emergency treatment is needed now!
Yankeeville hangs in the balance, awaiting news of DJ's injured left pinkie…
Oh, he'll play today against the Pirates?
Well, Nevermind!…as Roseanne Rosannadanna would say…
In an interview with WFAN, it appears that Mark Teixiera's signing with the Yankees wasn't so improbable after all. In words that are sure to infuriate both Angels and Red Sox fans, Tex clearly had the Yanks in his sights from the start.
It has been assumed that the Yankees came in with a late offer to swoop Tex away from the Red Sox. However, it appears that this was destined to happen from the start as the Yankees were apparently the only team that complied with Teixeira's request to keep negotiations confidential.
As transcribed by Mike DiGiovanna in the Los Angeles Times:
"I always had the Yankees in my sights, and one thing my agent and I had asked every single team is, we're not going to negotiate through the media, and we ask you not to. The Yankees were really the only team that did that. The other teams went out and told everybody their offers, told everybody that they talked to me, that they made this call, made that trip. The Yankees kept quiet. We talked all winter. When they were ready to make their final offer, it was a great offer, and my wife and I were excited about going to New York."
Teixiera was asked about the recruiting visit made to his Texas home by the Red Sox in December, and he responded: "I think in the end, it probably worked against them a little bit, because everyone thought the Red Sox were my No. 1 choice," Teixeira said. "… The Yankees had a leg up all along."
Regardless of how it went down, I will be glad to see Tex standing at first base for Opening Day at the new Yankee Stadium on April 16th.
Jared Max/WCBS
The Yankees will dedicate a new memorial for the victims of 9/11 today at Steinbrenner Field. A piece of steel from the World Trade Center ruins was used to create the memorial which depicts the Twin Towers sitting on a Pentagon-shaped foundation. The memorial sits on grass in tribute to United Flight 93 which crashed in Somerset County, PA. It is a tremendous memorial that pays a great tribute to all those who lost their lives on that fateful day, and broke the heart of a nation.
Courtesy New York Yankees
According to many articles, it sounds as though the Yankees may opt to go with Jonathan Albaladejo in the bullpen, leaving long reliever candidates Brett Tomko, Alfredo Aceves, and Dan Giese on the outside looking in. It's unfortunate for Tomko, who has had a terrific spring, but the odds were stacked against him from the start since he is not on the 40-man roster.
Kathy Willens/AP
I had expected the Minnesota Twins to return Rule 5 draftee Jason Jones to the Yankees, but the teams opted to swap righthanders as the Yankees received 23-year-old Charles Nolte. I don't know anything about Nolte, but he's three years younger than Jones. He posted a 2.05 ERA in 44 Single A relief appearances last year, and had 75 strikeouts in 70 1/3 innings.
With the attention that Ramiro Pena has drawn for his fielding prowess, is this the year that the Yankees start to experiment with Derek Jeter in an occasional start in other positions, like centerfield? Given that there are other long-term solutions for centerfield to go with Brett Gardner (like potentially Jeter and the much anticipated arrival of Austin Jackson later this year or in 2010), I wonder if Melky Cabrera will become a trading chip at some point. I am still not comfortable with the thought of Cody Ransom at third.
Where's Mike Pagliarulo when you need him?
Just kidding…
Speaking of Pena, it sounds as though he's beaten out Angel Berroa for the infield utility spot. I definitely feel for Joe Girardi. He has some very difficult decisions in the coming days, and they are decisions that will dramatically shape the 2009 Yankees. No pressure…
Meanwhile, I am trying to decide whether I want an apple or an orange. Man, what a tough decision! 😉


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My maternal 5th-great granduncle, Benjamin H. CROCKETT, married on this date in 1830 in Owen County, Indiana to Elizabeth THOMAS. Benjamin was the son of Alexander and Elizabeth (WATKINS) CROCKETT, and the younger brother of my David CROCKETT. I do not have Elizabeth's parentage. Benjamin was born in 1810. Elizabeth was born circa 1812. They had 8 children. Elizabeth died after the 1860 census. Benjamin died after 1880. Nothing further is known at this time.
My maternal 5th-great grandfather, James GROENENDYKE, married on this date in 1826 in Henry County, Indiana to Barbara BUCK. James was the son of Nicholas GROENENDYKE. Barbara's parentage is unknown. James was born in 1770 in Middlesex County, New Jersey. Barbara was born April 5, 1795 in Salem, North Carolina. They had 7 children. Barbara died September 17, 1878. James died September 1836 in Fayette County, Indiana.
Thursday, June 03, 2010
Family Milestones: June 3rd
at Thursday, June 03, 2010 Posted by Travis LeMaster
Labels: Buck, Crockett, Groenendyke, Thomas, Watkins
2 comments:
- Earline Hines Bradt said...
I guess that makes us cousins of some sort, my 5th great-grandmother, Elizabeth Hawkins Stonecipher,was first cousin to Davy Crockett on his mother's side.
- Travis LeMaster said...
Earline, there must be a connection there somewhere.
I'm seen some work on cementing the connection between my David to the Alamo Davy, but don't have it in front of me. Will keep looking.
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Known for his fusion of the International Style and personal poetic influences in his architecture,Louis Kahn is notably one of the most respected architects of the 20th century. He often worked alongside engineers and contractors, which enabled his innovative designs to be structurally sound while continually advancing towards a new refinement.
One of his more famous structures and the first significant commission of Louis Kahn, the Yale University Art Gallery in New Haven, Connecticut was designed when he was a visiting critic at the Yale School of Architecture as the first of three art museums to be designed and built. The project was built between 1951 and 1953.
With this Kahn was able to explore the ideas he had about transforming modern architecture which to him lacked the monumental and spiritual quality of ancient buildings. He was successful in his hopes of redefining architecture, as this building marks a significant turning point in the history of American museum architecture.
According to author Patricia Cummings Loud, "The commission brought about Kahn's discovery of structure, materials, and perhaps most important, the power of the forms he was capable of creating. The Yale Art Center served to catalyze many of his basic ideas and beliefs about architecture, both in words and in work."
This art gallery is considered by historians to be a response to Kahn's desire for a new monumentality in the post-World War II period. His masterful sense of space and light worked to create structures that emotionally impacted those who encountered them, as he combined visually compelling spaces that varied under the transforming light during different times of the day.While walking along the bordering street of the campus, the building's blank walls stand out against the neo-Gothic background of the university. As is apparent in this structure, Kahn typically tended toward heavily textured brick and bare concrete, which he wonderfully juxtaposes against more refined and pristine surfaces, like the large glass windows of this building beautifully lined by steel.The front door is found in a recessed corner that is defined by an absent rectangle following the pattern of the glass fenestration. The door leads to a series of open loft spaces on the first floor, which flow horizontally until the space is broken by core circulation elements, including the main stair, elevator and mechanical core. The highly flexible space not only stores a portion of the University's art collection but also functions as a studio for architecture students.The more prominent features of this building include the hollow concrete tetrahedral space-frame that allows for the omission of ductwork while also reducing the standard requirements regarding floor-to-floor height. His interest in pushing the boundaries with technology led him to design this waffle-slab that served as the floor of one room and just as functionally became the ceiling of another. The stairway in the center reflects the triangular patterns and lines of the exterior and also acts as an sculpture in the center of the gallery space.
Recently the art gallery underwent a three-year, $44 million renovation which hoped to restore the landmark to its original purity and integrity after years of repair and alterations. The hopes were to also update the building systems so that they may be of the most functional and optimal design.
The most laudable aspect of the renovation was the replacement of the signature window-walls of the building with a new system that addressed the original wall's technical shortcomings.The open-space layout of the galleries and the exterior courtyard were reinstated in the renovation, as well as the transformation of the first-floor lobby into a media lounge. The President of Yale University, Richard C. Levin says that the renovation "preserves and restores the architect's brilliant vision, and it also accommodates the Gallery's expanding scope and needs for many years to come."
Said by New York architect Jeffry Kieffer, "Kahn's accomplishment was not the formal variation of elements as ends in themselves, but his constant ability to extract from this void means to express his belief in the institutions he was working for."
Architect: Louis Kahn
Location: New Haven, Connecticut
Project Year: 1951-1953
Photographs: Flickr: caprilemon, illa flaubert; Elizabeth Felicella, images.library.yale.edu
References: greatbuildings.com, nytimes.com


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1971 Pro Street Dodge Challenger
By the time the ponycar wars were beginning, Keith Legar was following a family trend set by his father and brothers in developing a taste for Mopars. Keith remembers his dad owning nothing but Dodge or Plymouth station wagons, and his older brothers both had hot-rodded Plymouths as well. "Steve had a Savoy with a dual-quad 361 that we used to race, and Tommy worked on cars too," Keith says.
Keith purchased his first Mopar in the late '60s--a '63 Plymouth Sport Fury convertible with a 383 and a four-speed. He had big dreams for the car and even traded a Ducati motorcycle for a 426 Max Wedge that he planned to install in it. But then cruel reality reared its ugly head--somebody stole the seats out of the Plymouth. Keith gave up on the project and sold the car in frustration.
Thirty years later, Keith owns a construction-site clean-up business and has a son of his own. While he was working on a job site one day, he spotted a good-looking '71 Challenger parked in front of a neighboring home. He asked about the car and was told the owner didn't want to sell, so he went back to work and put the car out of his mind. About a year later, he ran into the homeowner at a hardware store and was told the Challenger was up for sale. Keith was soon back in the Mopar fraternity.
The '71 was an exceptionally clean 318/automatic car, and Keith drove it just as he bought it for a time. Then he started making some changes, adding an R/T hood and a 10.0:1-compression 340, and eventually treating the car to a full Alston backhalf kit with a 'cage and four-link rear suspension. Then a Six Pack hood was added, and to live up to the power it implied, the same 340 was recammed by Keith's pal John Gilbert, and treated to a D-1SC Procharger centrifugal blower; when the SD Performance blower mount wouldn't clear the downtubes of the rollcage, a custom mount was fabbed. But that didn't last long. Gilbert told us, "That 340 was just never built for blower power, and the bottom end fell out of it." So Keith scored a used R3 siamese-bore small-block at SoCal's Spring Fling Mopar show, and John loaded it with with billet guts and 8.5:1 compression for 427 ci of small-block Mopar that could withstand all the abuse the Motec-injected, intercooled Procharger could dish out. The end result is the car he now exhibits at local shows and drives a couple of times a week just for fun.
We joined Keith, his son Marty, and their friend Kareem Elsherif at a recent test-and-tune at Irwindale Raceway not far from his home in Redondo Beach, California. Elsherif, who is a Honda aficionado and has crewed for import racer Stephan Papadakis, served as the tuner for the injection system and eventually laid down the best numbers in the Challenger for that night. Without the transbrake, Elsherif posted a 6.86-second e.t. at 100 mph on the eighth-mile run, which means it should be good for high 10s in the quarter. But there's plenty more left, as the ribbed blower belt was slipping, allowing boost to drop from 8 pounds in First gear to just 4 at the top end. A cog beltdrive is in the works. Also, with 4.56:1 gears and 29-inch rubber, the engine spins 5,300 through the lights in the eighth-mile, so a set of 3.91s is being installed in preparation for estimated 135-mph trap speeds in the quarter.
So while the quest continues, Keith is still perfectly happy that he finally was able to build a car the way he wanted it and keep his family's Mopar tradition alive.
What: '71 Dodge ChallengerOwner: Keith Legar, a man with a Mopar pastHometown: Redondo Beach, California, where the sun and the surf make car guys smile
Short-Block: The block was a used R3 race version that he bought at a car show, and Keith's friend John Gilbert took it to 427 ci with a 4.125-inch bore and 4.00-inch stroke. The holes are stuffed with custom JE dished pistons for 8.5:1 compression. The rods are Manley H-beams with Chevy 0.927-inch pins and they swing on a Moldex billet crank. Milodon provides the oiling, and the cam is a mystery solid flat-tappet grind.
Heads: The block shoulders a pair of Indy Cylinder Head 360-2 oval-port, W2-style heads with CNC-cut 230cc intake runners and 2.100/1.650-inch valves. They're fitted with Indy proprietary shaft rockers.
Induction: The D-1SC centrifugal Procharger supercharger is hung on a custom bracket and drive system with parts from SD Performance and The Supercharger Store. It's connected to the Procharger air-to-air intercooler with custom stainless-steel tubing. Boost runs 8-12 psi when the belt isn't slipping. The intake is an Indy unit with 83-lb/hr RC Engineering injectors and a Mopar Performance throttle-body with a hat from The Supercharger Store.
Fuel System: A Motec Systems USA electronic control unit controls the flow from a trunk-mounted JAZ 12-gallon fuel cell with twin electric Paxton fuel pumps and XRP braided stainless fuel hoses. A pair of nitrous bottles are also in the trunk, but he hasn't opened them yet.
Exhaust: Stock TTI headers feed 3.00-inch stainless tubing and a pair of Spin Tech mufflers.
Transmission: A Dodge 518 automatic transmission that was swapped in from a '92 pickup modified by ProTrans in Lancaster, California, with a reverse-pattern valve body, a transbrake, and a 3,200-stall converter from Continental Torque Converters. The trans is fitted with an electric overdrive that can be operated in any gear through a toggle switch, so the Challenger can be quite docile on the highway. It can also be shifted hard on the track through a Precision Performance Products air shifter. The bottle for the shifter is housed under the dash and can be adjusted with a chip to change the tranny's shift points. When launched with the transbrake, the stock cast U-joint yoke gave up, so the car is now fitted with billet yokes and an aluminum driveshaft from Inland Empire Driveline Service.
Chassis: It's stock in front but has an Alston back-halved rear with a four-link and Koni coilovers. The rolling stock consists of Mickey Thompson 26x7.50-15 front and 31x18.50-15 rear rubber wrapped around Center Line rims. The rear wheelwells were opened to get the car low without feeding the rubber to the fenders. The power is passed back through an Alston Fab 9 rearend that carries a 4.56:1 ring-and-pinion set, a Detroit Locker, and Currie axles.
Interior: The interior reflects the car's street/track dual purposes. The 12-point rollcage heads up the safety gear and is supplemented by a pair of Recaro seats fitted with Simpson harnesses. The black door panels, carpet, and headliner are the work of Geisens Automotive Upholstery in Laguna Hills, California, and an aluminum instrument insert was added to the stock dash. It carries a host of Auto Meter Pro Comp mechanical gauges, including monitors for voltage, boost pressure, fuel pressure, and water temperature as well as a tachometer and a speedometer.
Performance: Keith has never run it down the 1,320, but it recently turned 6.86 at 100 mph in the eighth-mile. Rear-wheel horsepower is estimated at 700. With the upgrades being planned, high 9s in the quarter are expected.