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Depending on the intensity of ambient light, the PR 26 rotating green laser is four times more visible to the human eye than other class III lasers, according to the company. Featuring a range of up to 650 ft, the laser includes a built-in alignment system with remote control that doubles as a receiver. Rated for use between -20°C and 45°C, the product also features a rechargeable battery pack and charger that provides 22 hr of continuous working time per full charge, a built-in shock warning system, and an adjustable wall bracket that allows the product's height to be set to the millimeter.
For more information, visit www.hilti.com.
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Bombers split against Tigers
The Barnum Bomber basketball teams hit the road again December 29, this time taking the ride to Pine River to take on the Tigers. The afternoon began with the Bomber girls taking the court first.
Cheyanne Johnson started the shooting for the Bombers, hitting from beyond the arc for the first of many of her three-pointers on the afternoon. The Bombers trailed by 10 points at halftime, 28-18, with 17 of the 18 points coming from Johnson with five shots from beyond the arc and two from the paint. The other point for the Bombers in the first half came from Lissa Warpula from the free throw line.
The Bombers were not able to keep up with the Tigers. Johnson struggled in the second half, scoring only once from beyond the arc. Daron Mainville finally got on the score board but only managed to put up 6 points on the afternoon, while Warpula added another point from the free throw line.
The Bombers fell in the end by a score of 61-27.
Bomber boys
The Bomber boys followed in the second game of the double header on the afternoon. Brandon Newman was the first to score for the Bombers. Daniel Warpula was on the board shortly afterward, and the two of them began to dominate the game for the Bombers.
Dakota Haley got into the scoring collum with three points in the first half. The Bombers first half scoring would finish with Zach Carlson going to the free throw line to put up 2 points just before halftime. The Bombers led the way into halftime with a score of 30-17.
The Bombers came out of halftime ready to continue their run against the Tigers. Newman once again began the scoring, striking right a way. Carlson also came out in the second half adding 8 points.
The game ended with the Bomber boys taking a commanding lead over the Tigers, 67-34. Warpula put up 24 points. Newman had 20 points. Carlson put up 10 points. Haley had 5 points. Tory Slater put up 2 points, followed by Mitchell Weller with 2 points, John Zymslony with 2 points and Chase Peterson with 2 points.


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Leonora Carrington dies at 94; a leading figure of the Surrealist movement
Leonora Carrington dies at 94; a leading figure of the Surrealist movement
Reporting from Mexico City -- She painted eerie rituals with cloaked figures in forests, dancing deer with trees growing from their backs, and breathing buildings that shot planets and stars.
Leonora Carrington, a leading figure of the Surrealist movement and perhaps the last great living Mexican artist, died Wednesday in Mexico City, the government said. She was 94.
Carrington painted, drew, made sculptures, and wrote fiction and plays over a career that spanned much of the 20th century and featured affairs and friendships with some of the greatest artists of her time. Surrealist painter Max Ernst was an early lover in Paris. Remedios Varo was a close friend and fellow female painter in the vibrant artist community of Mexico City in the 1940s and 1950s. Surrealist Andre Breton and filmmaker Luis Buñuel were also among her acquaintances and contemporaries, and she sometimes brushed up against the tumultuous lives of artists Diego Rivera and Frida Kahlo.
Carrington's style became recognizable worldwide, a combination of anthropomorphic whimsy and an undercurrent of shadowy darkness. Yet she often rejected the label "Surrealist," insisting instead that she painted what she observed in the magical space between the corporeal world and the subconscious.
Chilean filmmaker and actor Alejandro Jodorowsky, a later Surrealist, wrote of Carrington as one of his "witch" muses, yet she once remarked: "I didn't have time to be anybody's muse; I was too busy rebelling against my parents and learning to be an artist."
Carrington was born April 6, 1917, in Lancashire, England, to an upper-class family. She began smoking at age 11 (and appeared never to stop), was expelled from several schools and studied art in London. She moved to Paris in the 1930s and began exhibiting her work, joining the Surrealists. Eventually, as the Nazis marched across Europe, Carrington suffered a nervous breakdown and wound up in a psychiatric ward in Madrid.
In 1941, Carrington met a Mexican diplomat, Renato Leduc, whom she married in Lisbon. They traveled to New York and by 1942 the pair settled in Mexico City, divorcing soon thereafter. Carrington later married a photographer, Emerico Weisz, and with him had two sons, Gabriel and Pablo, both of whom survive her.
Carrington painted and wrote energetically in Mexico, inspired by the country's rich pre-Hispanic civilizations and the mythologies and occult knowledge of cultures from around the world. One of her best-known works is an enormous mural titled "The Magical World of the Maya," commissioned in the early 1960s for the National Museum of Anthropology. Her most famous written work, a 1974 fantastical novel titled "The Hearing Trumpet," includes an account of a feminist uprising in a women's retirement home.
Later in life, Carrington enjoyed accolades that in Mexico are reserved for the country's most admired artists. In 2005, she was awarded the National Prize of Sciences and Arts.
In 2008, an outdoor exhibit of her large-scale sculptures along the stately central boulevard Paseo de la Reforma became hugely popular. Elena Poniatowska, the Mexican writer and author of a novel based on Carrington's life, said in an interview with CNN's Spanish language service earlier this year that the artist maintained both a British and a Mexican sensibility throughout her life.
"Smoking, drinking English tea — in her kitchen there is a photo of Lady Di — you feel a bit like you're in England when you're with her," Poniatowska said. "But she loves Mexico deeply. Her son Pablo says she wouldn't be who she is without Mexico."
In a 2008 interview with The Times, Carrington responded with a fanciful spell of words at the thought of returning to Europe: "There are places I'd like to return to. But not as I was then but as I am now. 'Cause I'm trying to understand. And I've understood nothing."
The artist added: "One is born, one lives, one dies. What death is, I don't know."
Hernandez is a news assistant in the Los Angeles Times' Mexico City bureau.


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If so, just pick up a copy of James Tooley's The Beautiful Tree: A Personal Journey into How the World's Poorest People Are Educating Themselves.
The Tories have looked at the evidence amassed by James and his colleagues (see p. 36 of their new report) and concluded that the best way to advance education in developing countries is to encourage and support existing entrepreneurial schools that are already serving the poor. And if the polls are any guide, that will likely be official government policy in the U.K. before too long.
Congratulations to James, Pauline Dixon, and their wonderful team for bringing sanity to the development policy debate.


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A woman is paying for some purchases at Macy's.
As she reaches for her card, a TV remote control falls out of her purse.
The sales clerk asks, "Do you always carry your TV remote?"
"No." the woman says, "But my husband refused to come shopping with me today. I figured this was a great way to pay him back."


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January 2007
Figure 1.
The golden-mantled tree kangaroo or weimang (D. pulcherrimus) is only found in the Torricelli Mountains, Papua New Guinea.
© Photo: Tenkile Conservation Alliance.
In November and December, 2005, a team of field naturalists from Indonesia, America, England, and Australia carried out the first comprehensive biodiversity survey of the Foja Mountains, an isolated range in northern Papua, a province of western (Indonesian) New Guinea (see Figure 2).
Spending a month in the Fojas, the 20-person team inventoried plants, frogs, reptiles, butterflies, mammals, and birds, documenting more than 40 new species in this little-studied corner of the tropical world. These amazing discoveries were the culmination of years of effort and planning.
Figure 2.
The Foja Mountains on the island of New Guinea are host to a dazzling variety of new and rare animal species.
© 2006 Conservation International.
The senior project co-leader began planning this expedition in 1982—the 23-year project timeline should adequately convey a sense of the political challenges to obtaining permission for the field research as well as the nature of this area's near-absolute inaccessibility. The team members all agreed the stunning results proved the project was worth the long wait, and that all the hard work that went into making it happen was well and truly worthwhile.
The search for a lost world
The Fojas' story begins back in the mid-1890s, when a shipment of stuffed birds, intended to adorn women's hats, arrived in Europe from New Guinea. Some of the more peculiar specimens in this shipment were removed by the Dutch trader who received them and forwarded to prominent European naturalists. An unusual bowerbird was sent to Lord Walter Rothschild in England, and a black-and-white bird of paradise was forwarded to a German natural history collection. Shortly thereafter, these were described as new species by Rothschild and renowned ornithologist Otto Kleinschmidt, respectively. They noted the following:
- The bowerbird was distinct in sporting a large erectile crest of golden plumes that stretched from forehead to nape.
- The bird of paradise exhibited a curious mix of characters found in two already-described species of six-wired birds of paradise.
- Most importantly, neither specimen came from an identified locality, but it was assumed that both originated from somewhere in the mountains of western New Guinea, then a Dutch colony.
In the decades to follow, a number of ornithologists were to make expeditions to western New Guinea trying to discover the homeland of these two unique species. Certainly, the researchers wanted to know more about these two "lost" forms, but undoubtedly a greater motivating force was the thought that whoever found the homeland of these mysterious birds might find additional species new to science—the prime goal of most expeditions.
Those who ventured to New Guinea in search of this mountain habitat scoured a number of isolated mountain ranges and adjacent mountainous islands, but to no avail.1 The mystery of the golden-fronted bowerbird was finally solved in 1979 by biologist Jared Diamond, who helicoptered with a small team into the uplands of the Foja Mountains of northern Papua (then called Irian Jaya). He observed that this species
- creates distinctive terrestrial display bowers made of moss and sticks and decorated with blue and yellow fruit
- builds dozens of these bowers on mid-elevation ridge tops in the Fojas
Diamond's wonderful discovery was met with considerable coverage in the Western press, and his paper reporting the rediscovery of the bowerbird was featured as a cover article in Science.2 Diamond also collected evidence hinting that the missing bird of paradise might inhabit the Foja Mountains, but the solution to this mystery had to await our visit to the Fojas in 2005.
The 2005 expedition
I began making plans to conduct a comprehensive biological survey of the Foja Mountains shortly after Diamond's discovery. With this in mind, I visited Indonesia several times, made three overflights of the mountain range, and held discussions with a range of governmental and nongovernmental stakeholders. Thus began more than two decades of on-again, off-again efforts to pull all the pieces together to conduct such a complex, expensive, and difficult mission.
In 2003 I met with project co-leader Stephen Richards of the South Australian Museum to reformulate our plans in light of evidence of improvement in the political climate in Indonesia. Our redoubled efforts produced results in 2005, when our multi-institutional team received preliminary approval for our plan from Indonesia. We then pushed into high gear, pressing for government permits, making an extra effort to raise additional funds, and clearing personal travel schedules to make way for this once-in-a-lifetime opportunity. In October, local village landowners granted us their approval for the field trip. The international team arrived in Jakarta in November, and the final national and provincial authorizations were finalized shortly thereafter.
One final hurdle remained. The team would not be able to get into the misty montane uplands of the Fojas without a helicopter, and helicopters were rare and expensive in Papua in November 2005. Through some indirect negotiations with several institutional partners, the evangelical service organization Helimission agreed to a charter of one of its helicopters, providing its incomparable bush pilots for the challenging mountain-top drop-off and retrieval. At that point we were set to go.
The team and its copious supplies and equipment were ferried by a single-engine Cessna aircraft into the Kwerba airstrip at the foot of the mountain range in mid-November. We divided this large party into a hill forest survey group and a montane survey group:
- The hill group established camps on foot in the hill forest northeast of Kwerba.
- On 22 November, the mountain team got its helicopter ride up to a boggy clearing in the montane forest at 1,650 meters above sea level, in the heart of the Foja's interior.
Being at 1,650 meters in the Foja Mountains was a dream come true for the research team of six: botanist Wayne Takeuchi, lepidopterist Henk van Mastrigt, herpetologists Steve Richards and Burhan Tjaturadi, mammalogist Kris Helgen, and ornithologist Bruce Beehler. A 30-minute helicopter ride transported us into a montane forest tract of humid tropical forest that showed no evidence of human impact: no road, no trail, no trash, no village, no TV, no radio. Only rarely did a passenger jet overhead disturb our isolated wilderness environment. Our six local guides, from the villages of Kwerba and Papasena, were just as amazed as we were. They assured us that they had never visited this interior region of the Foja Range. And the wildlife in many instances was remarkably unwary (unusual in a place like New Guinea, where subsistence hunting is chronic and all pervasive). Birds flitted around our campsite and sang lustily from nearby trees. A giant rat visited nightly to collect scraps. Most remarkably, the long-lost bird of paradise carried out an elaborate display on the ground within view of our rough dining table on a drizzly afternoon.
Our team of 12 worked night and day for 15 days, to learn as much as we could about the natural history of this remarkably pristine and isolated mountain range.3 Whereas the hill forest team found that the forest in their area supported mainly common and widespread species (their five apparently new species of palms were an exception to this rule), our montane team found a world of biological novelties:
Figure 3.
Berlepsch's six-wired bird of paradise (Parotia berlepschi) is named for the curious wires that extend from its head in place of a crest.
© 2006 Conservation International, Bruce Beehler.
- up to 20 new species of frogs
- 5 to 10 new species of plants
- 5 new butterflies
- several possible new mammal species, including a large mammal (golden-mantled tree kangaroo, see Figure 1) new to the Indonesian national list
- a new bird species: the wattled smoky honeyeater
- the "lost" Berlepsch's bird of paradise (see Figure 3)
Moreover, we believe that there are dozens of additional new species from these focal taxonomic groups that will be found with additional effort.
A call for conservation
Finding new species in the tropical rainforest is no rare event. What, then, is so remarkable about this expedition and its findings? Why should we ensure this lost world's biodiversity remains intact?
- When coupled with the remarkable recent discovery of more than 50 new marine species in the waters just west of the Foja Mountains,4 the scale of the biodiversity discoveries coming from Indonesian New Guinea is remarkable. Conservation International, which sponsored and led both the marine and terrestrial field research, is proposing additional field studies, and these are clearly justified. Today, Papua might well be considered a "lost world" for novel biodiversity, and the situation is such that a province-wide biological survey (both marine and terrestrial) is warranted. Perhaps as little as 50 percent of New Guinea's frog species have been described.5
- This offers an opportunity for institutional collaboration and training of new field students, benefiting Indonesia and tropical field science. A large-scale multi-year program of field surveys, matching Indonesian and international scientists with local research students, would set the stage for a renaissance of field study in this important but little-studied part of the tropical world. This, in turn, could lead to greatly expanded ecotourism in the Province, providing local economic benefits.
- The expanded field research and ecotourism could drive a conservation-planning process that could help Papua design and manage a network of forest and marine parks that could become the envy of the tropical world. This would go a long way toward addressing the growing threats of poaching, over-hunting, and unconstrained industrial logging and plantation development. There is room for both development and nature conservation in Papua, if guided by thoughtful planning and management of these resources. It is not too late to make these steps.
The good news is that the Foja Mountains are already part of Indonesia's system of national wildlife sanctuaries. It should be noted, however, that the Fojas are also the traditional lands of several forest-dwelling peoples who inhabit the range's surrounding foothills. The forests constitute these communities' patrimony—their main source of wealth. From the upland forests of the Fojas the local people obtain
- their pure drinking water
- their wild game in the form of pigs, cassowaries, wallabies, and tree kangaroos
- fish from the rivers
- fiber and building materials from the forests
- stories, legends, and myths as a product of their long interaction with the hills, woodlands, plants, and animals
It is safe to say that these local forest peoples are the true stewards of the pristine forests of the Foja Mountains. Any sustainable conservation plan for this remarkable region must place these local stakeholders at the center of any future agreements and plans. These are the voices who can speak with authority about the forests, and these are the people in place to protect these natural resources from the relentless pressure of large-scale development that is bound to arrive at their doorstep in the decades to come. It is the mandate of conservation organizations, such as Conservation International and counterpart government agencies, to work closely with these local stakeholders for the good of this globally significant resource.
© 2007, American Institute of Biological Sciences. Educators have permission to reprint articles for classroom use; other users, please contact email@example.com for reprint permission. See reprint policy.


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An asterisk designates a term specific to IRONMAN-branded events.
The IRONMAN Run Glossary
5K: 3.1 mile run or race. Generally the distance in a sprint triathlon.
10K: 6.2 mile run or race. Generally the distance in an Olympic triathlon.
aerobic: This term is used to define the intensity of a run that is primarily conversational at a slow, easy pace. Generally, you burn
anaerobic: High-intensity pace that allows lactic acid to build up, and can generally not be sustained much longer than a 10K.
elastic laces: The "stretchy" shoelaces many triathletes have on their shoes to allow easy and fast entry into the shoe without having to tie a knot.
fartlek: A style of running that is "random" or variably paced. For example, a fartlek run might involve running five miles on a trail, and sprinting at various intervals throughout the run. Also known as "speed play."
hill repeats: When a runner runs up a hill to increase their strength, and down at an easy, recovery pace. Also used in cycling.
hitting the wall: Generally happens about mile 20 of a marathon due to depletion of carbohydrate. A drop in blood sugar leads to immediate fatigue and loss of energy. (Also called a "
intervals: Short, fast repeats of 30 seconds to five minutes, interspersed with easy walking or jogging in between each effort.
marathon: 26.2 miles. Generally the distance in an IRONMAN triathlon (a half-marathon is the distance in an IRONMAN 70.3 event.)
negative split: Running the second half of a run faster than the first half.
pick-ups: Short accelerations performed during the run, generally to stretch out the legs and prepare them for speed work or a run. Usually 10-30 seconds long.
plyometrics: Jumping, bounding, hopping or other explosive movements designed to train the body for reducing ground contact time.
pronation: The inward roll of the foot as the arch collapses after the foot strikes the ground. Over pronation is excessive inward rolling due to weak support, which can cause many running injuries.
runner's high: An intense feeling of exhilaration that can occur during a run, usually due to the release of endorphins.
splits: in running, refers to your times at mile markers or other pre-planned distance markers.
strides: Similar to pick-ups, but usually performed as intervals (i.e. a set of 8 strides to warm up prior to a race).
supination: Opposite of pronation. Outward rolling of the foot after foot strike. Less common, but also a cause of running injuries.
If you want to learn more about
The IRONMAN Swim Glossary | The IRONMAN Bike Glossary | Run Glossary


text:
Gov. Dayton signed the construction projects bill on Tuesday, meaning the project is a go. When I return from vacation, I plan to do a more detailed story on the hockey portion of the civic center project. Meantime, here's how it's supposed to look:
|Here's a closer look at the west side.
|A new second floor on the west side of the rink will have a training/conditioning (weight room) area, as well as a kitchen and dining area for the hockey teams.


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Error: Learn about refurbishment in the Hippy Dippy Pool area. Learn about refurbishment in the Hippy Dippy Pool area. See Details
Opening September 29, 2019, this new gondola system will offer a bird's-eye view as it whisks you and your family away to Epcot and Disney's Hollywood Studios.
Immerse yourself in the spirit of the 1960's at the sprawling Hippy Dippy Pool, which features flower-shaped water jets. For the little ones, a kiddie pool area with a pop-jet water fountain is located nearby.
Two more pools, the smaller 1950's Bowling Pool and the 1990's Computer Pool, are also on hand for Guests looking to take the pop-culture plunge.
Shop for Disney merchandise before or after you dine at this far-out food court that's a blast from the past. Find food favorites like waffles, pasta, flatbread and salads in one of the 7 food stations.
In-room pizza delivery is also available for those looking to dine in.
Make your vacation more unforgettable with a personalized in-room celebration—featuring gifts, treats and décor.
Per Night Excl Tax
(407) 938-4000


text:
Don't even think that you have a more difficult job than Tom Horton. Although some airline bosses can still seem besotted with this once glamorous business, at ground level it's an industry that has lost more than $60 billion in the past decade, erasing every penny earned in its 80-year existence. An industry whose combined market value, below $30 billion, is less than that of Starbucks, one of its vendors. One with irrational competition, uncontrollable fuel costs, a feckless regulator, and jets and airports filled with people who don't like you--and not just the passengers but your employees too. Beyond that,...
The Worst Job In America
Beset by bankruptcy, balky unions and an unwanted suitor, American Airlines' CEO is trying to set a new course for the company
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Breifcases - Choose Your Weapon for Corporate Combat
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Related Product Information
Enrich untouched mouse Dendritic Cells (DCs) by depleting T cells, mIgM+ B cells, NK cells, erythrocytes and most granulocytes from mouse spleen or lymph node cells with this product. The DC enriched population is bead- and antibody-free and intended for further isolation of any DC subpopulation by flow sorting.
DCs in the enriched population are characterized by expression of CD11c and absence of lineage markers other than those co-plasmacytoid DCs. This kit provides high recovery of CD11c+ cells suitable for further FACS enrichment of any subpopulation, including DCs positive for CD4, CD8a, B220 and CD19 (1-4).
Principle of Isolation
Add a mixture of biotinylated monoclonal antibodies against non-DC cells to your starting sample. Add Depletion MyOne™ SA Dynabeads and allow them to bind to the non-DCs during a short incubation. Separate the beadbound cells with a magnet. Discard the bead-bound cells and use the remaining untouched, enriched cell population for further flow sorting into the DC subpopulation(s) of interest
Description of Materials
Materials Supplied
- 5 ml Depletion MyOne SA Dynabeads -- Depletion MyOne SA Dynabeads are uniform, superparamagnetic polystyrene beads (1.0 μm diameter) coated with streptavidin (SA). Supplied at 10 mg beads per ml in phosphate buffered saline (PBS), pH 7.4, containing 0.1% bovine serum albumin (BSA) and 0.02% sodium azide.
- 1 ml Antibody Mix for Mouse DC kit -- The Antibody Mix contains an optimized mixture of biotinylated monoclonal antibodies against mouse CD2, CD3ε, CD49b, mIgM and Ter-119 supplied in phosphate buffered saline (PBS), pH 7.4, containing 0.02% sodium azide.
- The kit will process approximately 4 x 109 leucocytes, corresponding to 5 x 108 density gradient-prepared leucocytes.
Additional Materials Required
- Magnet (Dynal MPC™): See magnet recommendations.
- Mixer allowing both tilting and rotation.
- Buffer 1: PBS (without Ca2+ and Mg2+) w/0.1% BSA and 2 mM EDTA, pH 7.4.
- Buffer 2: PBS (with Ca2+ and Mg2+), and 0.1% BSA, pH 7.4
- DNAse
- Density gradient medium – (e.g., Nycodenz® 1.077 g/cm3 or equivalent)
- Cell strainer, 70 μm
Important Notes:
- It is critical to keep the buffers cold (2-8˚C) during the enrichment steps.
- PBS containing Ca2+ or Mg2+ is not recommended, except when required in DNAse treatment (as specified in protocol).
- Do not use buffers or additives (i.e. FCS) containing biotin since this may reduce efficiency of depletion.
- Use a mixer that provides tilting and rotation of the tubes to ensure Dynabeads do not settle at the bottom of the tube.
- Follow the magnet recommendations to ensure a successful isolation.
Dynabeads should be washed before use.
- Resuspend the Dynabeads in the vial.
- Transfer the desired volume of Dynabeads to a tube.
- Add the same volume of Buffer 1, or at least 1 ml, and mix.
- Place the tube in a magnet for 3 min and discard the supernatant.
- Remove the tube from the magnet and resuspend the washed Dynabeads in the same volume of Buffer 1 as the initial volume of Dynabeads (step 2).
Sample Preparation
As the enriched DC population is intended for further isolation of subpopulations by flow sorting we advise running single cell suspensions of spleen or lymph node cells through a density gradient to remove platelets, cell debris and high density cells (2).
Recommended Protocol for Preparing Starting Sample for Enrichment.
- This protocol is based on isolation from a maximum of 5 spleens.
- Remove the spleens or lymph nodes from recently killed mice and transfer to a 50 ml tube containing 30 ml room tempered Buffer 2 supplemented with 120 IU/ml DNAse.
- Crush the tissues through a 70 μm cell strainer into a new tube. Flush the cell strainer with the initial buffer to wash out all cells.
- Incubate the tube for 15 min at room temperature (RT) with tilting and rotation.
- Add 0.2 ml of 0.5 M EDTA to each tube to dissolve any DC-T cell aggregates.
- Mix and incubate for 5 min on a roller at RT with tilting and rotation. Perform further cell processing on ice with cold buffers.
- Filter the cells through a new 70 μm cell strainer into a new 50 ml tube and flush the strainer with Buffer 1.
- Fill the tube with Buffer 1.
- Centrifuge at 300 x g for 10 min at 2-8°C.
- Discard the supernatant and resuspend the cell pellet in 5 ml of Buffer 1.
- Transfer the cell suspension and layer carefully on top of 4 ml density gradient medium in a 15 ml tube.
- Centrifuge at 1700 x g for 10 min at 2-8°C with slow acceleration and no brakes.
- Carefully transfer the leucocyte layer to a new tube.
- Fill the tube with Buffer 1.
- Centrifuge at 300 x g for 10 min at 2-8°C.
- Count the cells and resuspend in Buffer 1 to a concentration of 1 x 108 cells/ml.
Enrichment of Mouse DCs
This protocol is based on enrichment from 1 x 10 7 leucocytes. It is scalable from 1 x 10 7-5 x 10 8 cells, (see table 1).
Keep the cells and buffers cold (2-8°C) during the whole process.
- Transfer 100 μl (1 x 107) leucocytes in Buffer 1 to a tube.
- Add 20 μl of Antibody Mix.
- Mix well and incubate for 20 min at 2-8°C.
- Wash the cells by adding 2 ml Buffer 1. Mix well by tilting the tube several times and centrifuge at 300 x g for 10 min at 2-8°C. Discard the supernatant.
- Resuspend the cells in 900 μl Buffer 1.
- Add 100 μl pre-washed Depletion MyOne SA Dynabeads.
- Incubate for 15 min at 2-8 °C with gentle tilting and rotation.
- Resuspend the bead-bound cells by thorough pipetting or vortex for 5 secs.
- Add 1 ml Buffer 1.
- Place the tube in the magnet for 3 min.
- Transfer the supernatant to a new tube. The supernatant contains the DC enriched cell population.
Downstream Applications
The DC enriched cell population can be used for further isolation of DC subpopulations to high purity using flow sorting.
Table 1. Volume requirements for mouse DC enrichment.
|Working volume per 1 x 107 leucocytes
|Working volumes per
1 x 107 leucocytes 1 x 108 leucocytes
|Cell volume (step 1)
|100 μl
|1 ml
|Antibody Mix (step 2)
|20 μl
|200 μl
|Washing (step 4)
|2 ml
|20 ml
|Resuspension (step 5)
|900 μl
|9 ml
|Depletion MyOne SA Dynabeads
(step 6)
|100 μl
1 ml
|Volume added before magnet (step 9)
|1 ml
|10 ml
|Dynal MPC recommended
When working with other cell numbers, scale up all reagents and volumes accordingly. As a rule of thumb, up to 5 x 10 7 leucocytes can be processed in a single 15 ml tube and up to 2 x 10 8 leucocytes can be processed in a single 50 ml tube.
Invitrogen Dynal AS complies with the Quality System Standards ISO 9001:2000 and ISO 13485:2003.
Description of Materials
Dynabeads FlowComp™ are uniform, superparamagnetic beads (2.8 μm in diameter). Supplied at a concentration of approx. 1 × 109 beads (10 mg) per ml in phosphate buffered saline (PBS), pH 7.4, containing 0.1% bovine serum albumin (BSA) and 0.02% sodium azide (NaN3) as preservatives.
This product is stable until the expiry date stated on the label when stored unopened at 2-8°C. Store opened vials at 2-8°C and avoid bacterial contamination. Keep Dynabeads in liquid suspension during storage and all handling steps, as drying will result in reduced performance. Resuspend well before use.
Warnings And Limitations
This product is for research use only. Not intended for any animal or human therapeutic or diagnostic use unless otherwise stated. Follow appropriate laboratory guidelines. This product contains 0.02% sodium azide as a preservative, which is cytotoxic.
Avoid pipetting by mouth!
Sodium azide may react with lead and copper plumbing to form highly explosive metal azides. When disposing through plumbing drains, flush with large volumes of water to prevent azide build up. Certificate of Analysis (CoA) is available upon request. Material Safety Data Sheet (MSDS) is available at .
- Shortman K and Liu Y (2002) Mouse and human dendritic cell subtypes. Nature Rev. Imm. 2:151-161.
- Henri S et al (2001) The dendritic cell populations of mouse lymph nodes. J. Imm. 167: 741-48.
- Vremec D et al (2000) CD4 and CD8 expression by dendritic cell subtypes in mouse thymus and spleen. J. Imm. 164: 2978-86.
- Baban B et al (2005) A minor population of splenic dendritic cells expressing CD19 mediates IDO-dependent T cell suppression via type I IFN signaling following B7 ligation. Int. Imm. 17(7): 909-19.
For Research Use Only. Not for use in diagnostic procedures.


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A look at the Hollywood icon renowned for being economical with words, but never with style.
Laconic, effortless and the man who stole the heart of Ms Lauren Bacall, Mr Humphrey Bogart was the most insouciant actor of his time. In strong contrast to present-day Hollywood stars, he never ran towards the cameras with a fireball a feet or two behind him and he never used two words if one would do. In fact, most of Mr Bogart's great movie moments - and there were many - gained their strength from the fact that he said virtually nothing at all, capable as he was of saying everything necessary by the way he lit a cigarette or gazed ahead - nothing more overtly dramatic than that.
"The expression 'laid-back' could have been invented for the one they called Bogie and the truth is they just don't make them like that any more."
But he wasn't entirely as he seemed. Although he became the archetype of the American tough guy and a symbol of US egalitarianism between the wars, Mr Bogart was not a man of the people. His family was comfortably off when he was young and he went to good schools - although he was expelled in 1918 in a scenario evocative of The Catcher in the Rye. Even though Mr Bogart's background wasn't entirely one of silver-spoon assurance, it was comfortable enough to give him the laid-back air that was to be the backbone of his performances on screen as well as in life. And that was the reason why Bogie had such a huge effect on men everywhere. Quite simply, they wanted to be like him because they loved his confidence and cool authority, and the effect they had on women, on and off the screen. In fact, Mr Bogart was the creator of a school of acting, influencing stars such as Messrs Jean-Paul Belmondo and Yves Montand, who admired the sexual charisma that came naturally to Mr Bogart but had to be learnt by others.
Mr Bogart's acting career had a shaky start. He began in the theatre, with varying success, but it was Hollywood that made him, with its gritty film noir approach to its superheroes who were hard on the outside but soft on the inside and, despite an apparent indifference to women, were totally in love with them. Men of undemonstrative gestures and few words, their one-liners not only thrilled audiences at the time, but they remain potent today - and in no case more clearly than that of Bogie. There are certain expressions that, although associated for all time with him, have become part of the lexicon of language wherever English is spoken. Perhaps the most perfect examples of Bogieisms are from Casablanca, the 1942 film in which he starred with Ms Ingrid Bergman. "Here's looking at you, kid", and, inevitably, "Play it again, Sam" which (although a misquote from the actual words in the film, which are, "Play it, Sam") are known to us all.
After three marriages, Mr Bogart met Ms Lauren Bacall, who became as much an icon of American glamour as he was. If it wasn't exactly love at first sight then it was very near it, and the sexiest couple in Hollywood was created - and, like their marriage, it lasted until Mr Bogart's death from esophageal cancer in 1957.
|Ms Bacall, Mr Bogart, Mr Walter Brennan and Mr Howard Hawks on the set of To Have and Have Not, 1944
What was the secret of Mr Bogart's appeal? Most importantly, he interested men as well as women. They took him as a template for US masculinity. He stood for the honesty and integrity of ordinary, decent men. American men, whether middle-class professionals or blue-collar workers who found their identity in his laid-back approach to dress and gesture on and off the screen. "Less is more" is a mantra that could have been created with Bogie in mind. And that was why he was always so totally convincing in the wide variety of roles he played. The clipped comment, the laconic movement: it is no exaggeration to say that they were the basis of masculine America for at least two decades, lasting unquestioned until the age of defiant youth in the early 1950s when Messrs Marlon Brando and James Dean became the new heroes. But even then, from his fedora to his casually belted trench coat, Mr Bogart was still about the confidence that means you don't have to try too hard.
Six decades after his death there is still no hope that there will ever be a star who could be dubbed the new Humphrey Bogart. His world has gone but his memory remains.
Words and voice-over by Mr Colin McDowell, MR PORTER.COM


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Government conservation agency Scottish Natural Heritage has published a map identifying 43 areas of land in the country's remoter upland, mountain and coastal areas which "have little or no capacity to accept new developments".
At the same time, the Scottish government published a draft permitting policy which states that wind farms will not be permitted in national parks. This is the first time this has been expressly enshrined in law, but there are no wind farms in national parks in Scotland.
Together, the proposals mean that 31% of Scotland's land mass will have strengthened environmental protection against wind farm developments, which includes the 19% of land covered by national parks.
Jenny Hogan, director of policy for Scottish Renewables voiced concerns that excluding large areas of the country suitable for wind farms could potentially slow progress towards meeting Scotland's renewable energy targets.
She said: "Ultimately decisions over where wind farms can and can't be built are for democratically elected politicians both locally and nationally.
"It is important that the consultation on the planning proposals brings greater clarity to all those concerned with making decisions about Scotland's landscapes and power needs."
The policy also confirmed that local councils cannot impose moratoria on onshore wind development.
This follows requests last year from two local authorities in Scotland for a temporary halt to the construction of more wind farms after they claimed to have been inundated with "opportunistic" applications.
The government rejected their requests, claiming it would cause undue delay to developments.


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This section explains the policy attributes that govern password content.
Although password values are not generally returned in directory searches, an attacker could potentially gain access to the directory database. Therefore, password values are generally stored in one of the supported hashed formats that you specify using passwordStorageScheme(5dsat).
You can also enforce a check that passwords meet your definition of minimum password quality, by setting pwdCheckQuality(5dsat). The server then checks that the password does not match any of the values of the cn, givenName, mail, ou, sn, or uid attributes. The comparison of the password with any of these attributes is case-insensitive.
Additional checks are available with pwdCheckQuality(5dsat) set. You can enforce that passwords have at least a specified number of characters by setting pwdMinLength(5dsat). Furthermore, when the Strong Password Check plug-in is enabled, Directory Server checks that the password does not contain strings from the dictionary file that the plug-in uses. The server also checks that the password contains an appropriate mix of different types of characters.
You can enable strong password checking with the dsconf set-server-prop command. Use the pwd-strong-check-enabled property to turn on the plug-in, and restart the server for the change to take effect. Use the pwd-strong-check-require-charset property to specify what character sets to require in passwords. The pwd-strong-check-require-charset property takes a mask of the following values:
The new password must include a lower case character.
The new password must include an upper case character.
The new password must include a digit.
The new password must include a special character.
The new password must include at least one character from each of at least two of the above mentioned character sets.
The new password must include at least one character from each of at least three of the above mentioned character sets.
The default setting for the pwd-strong-check-require-charset property is lower && upper && digit && special.


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Hurricanes return home to host Bluesby Sean McCullen
BLUES (36-11-5) at HURRICANES (24-20-9)
Last 10: St. Louis 6-4-0; Carolina 6-4-0
Season series: The St. Louis Blues beat the Carolina Hurricanes 4-2 at Scottrade Center on Nov. 16 in the first of two games between the teams.
Big story: With a win in Raleigh, the Blues would pull even with the Chicago Blackhawks atop the Central Division standings at 79 points with three games in hand. The Hurricanes would jump over the Columbus Blue Jackets and Philadelphia Flyers into third place in the Metropolitan Division with a win against the Blues.
Team Scope:
Blues: St. Louis has won three in a row since being routed by the New Jersey Devils 7-1 in snowy Newark on Jan. 21. The Blues got a measure of revenge against the Devils on Tuesday, when Jaroslav Halak made 23 saves in a 3-0 shutout.
On Tuesday, T.J. Oshie and Jaden Schwartz switched lines, with Oshie joining Patrik Berglund and Magnus Paajarvi as Schwartz skated with Oshie's regular linemates, David Backes and Alexander Steen. The team used the new lines in spurts during its four-game road trip before going to them predominantly in the rematch with New Jersey.
Coach Ken Hitchcock enjoys the flexibility Oshie offers him when putting together his lineup.
"I think, depending on the game, depending on the score, that's the flexibility we work with," Hitchcock said, according to the St. Louis Post-Dispatch. "We did it against the [New York] Islanders a little bit, we did it a couple of shifts against the [New York] Rangers. I think you're going to see constant movement there all year. … Oshie is the guy who can play left, right and center. He can play in a checking role or a scoring role. Oshie's the guy who can provide us with flexibility."
Hurricanes: Carolina's four-game winning streak came to an end in a 3-0 road loss to the Montreal Canadiens on Monday. The Hurricanes return home to PNC Arena, where they are 13-10-5 overall and 5-2-0 in 2014, for their next four games.
Goalie Cam Ward, who has missed the past 13 games with a lower-body injury, could return at the end of the homestand. He practiced with the team Thursday after being activated off injured reserve, but was assigned to the Charlotte Checkers of the American Hockey League for a conditioning stint.
Ward will likely play a game or two with the Checkers, coach Kirk Muller said.
"I think it's important that he's going to go down and play a couple of games with Charlotte," Muller said. "I think that will be great for him for the big reason that health-wise … this is his first practice that he's had, so we gave him the net for the full 40 minutes to get the timing and endurance back. The opportunity to go down there and play will get him in a good mindset of where he feels. The biggest thing health-wise is that he can get through a game and he feels good and he feels comfortable."
Who's hot: Halak has won his past three starts with a 1.31 goals-against average and 90 saves on 94 shots (.957 save percentage). … Hurricanes defenseman Andrej Sekera has five assists and six points in the past five games.
Injury report: Steen (lower body) has missed the past few practices, but will likely play. … With the exception of Ward, Carolina reports a healthy lineup.


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Deportation of Immigrant in Same-Sex Marriage Suspended
A gay Venezuelan man married to an American man will temporarily not be deported after an immigration judge ruled Friday to suspend his deportation based on the recent intervention of U.S. Attorney General Eric Holder Jr. in a similar same-sex marriage immigration case.
Judge Alberto J. Riefkohl of Newark, N.J. suspended the deportation of Venezuelan salsa dancer, Henry Velandia, married to Princeton graduate student, Josh Vandiver, because Holder on Thursday intervened and suspended the deportation of a man from Ireland who is joined in a civil union with a U.S. citizen.
Holder suspended the deportation of Irishman Paul Wilson Dorman and sent his case back to the immigration appeals court, asking it to consider several possible grounds on which the Irishman might qualify for legal residency.
Both Dorman, who is joined to a U.S. citizen through a civil union, and Valandia were denied legal residency because of the Defense of Marriage of Act defines marriage as between a man and a woman and therefore does not recognize same-sex marriage.
Judge Riefkohl said he chose to delay Velandia's deportation to December, at the earliest, to allow time for the attorney general and the appeals court to work out a similar deal for the Venezuelan.
The chairman of the House Judiciary Committee, Lamar Smith (R-Texas), criticized Attorney General Eric Holder of having "instructed an immigration court to ignore DOMA in future rulings," according to The New York Times.
In February, President Barack Obama issued an executive order to the Department of Justice to cease defending the 1996 law signed by President Bill Clinton.
While the DOJ cannot defend the constitutionality of DOMA in court, it still must obey the federal law. Moreover, DOMA has not been struck down either legislatively or in the courts and therefore is still a federal law that must be enforced.
But in March, the U.S. Citizenship and Immigration Services wrongly issued a temporary suspension on deportation cases of gay foreigners joined in same-sex marriages to American citizens.
"USCIS has issued guidance to the field, asking that related cases be held in abeyance, while awaiting final guidance related to distinct legal issues," said Christopher S. Bentley, the Department of Homeland Security's spokesman, in a statement.
But Mathew Staver, founder and chairman of Liberty Counsel, denounced the USCIS's decision.
"[DOMA] governs the definition of marriage from a federal standpoint under the federal laws for every federal agency including the immigration services. They must abide by that federal law," he insisted. "No federal agency can act contrary to that law."
Bentley later told The Christian Post that the agency lifted the abeyance and would continue with deportation proceedings for gay foreign nationals regardless if they are in a same-sex union. The agency also criticized media outlets' assertion that married gay foreigners could now apply for green cards as untrue.
American Immigration Lawyers Association told CP that it too would not counsel gay foreign nationals joined to a same-sex citizen to apply for a green card. AILA spokeswoman Jenny Levy said gay foreign nationals who apply should not expect approval for their green card applications.


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Miami Heat
Miami Heat falls to new-look Brooklyn Nets in another forgettable exhibition
Nets coach Jason Kidd will miss Brooklyn's regular-season home opener against the Heat as part of his league-mandated punishment for driving drunk. So, on Thursday at Barclays Center, the Nets retired his jersey before a preseason game.
Other than perhaps a hard foul by Paul Pierce on LeBron James, the retirement ceremony of the Nets' new coach and greatest player in franchise history was the most memorable thing about a mostly unforgettable night at Barclays Center. It was the first clash between the new-look Nets and the back-to-back defending NBA champions and it was terrible even by preseason-game standards. The Nets defeated the Heat 86-62.
Miami's regular-season opener against the Bulls on Oct. 29 can't get here fast enough, but, alas, there are still three more preseason games on the Heat's schedule. A few signs the Heat has played two too many preseason games already:
• Miami has averaged 72 points in the past two games, both road losses.
• Led by a frontcourt of James Jones, Joel Anthony and Jarvis Varnado, the Heat scored 12 points in the second quarter Thursday.
• Shane Battier started the game opposite Kevin Garnett, a slight mismatch.
• Mario Chalmers is 2 of 17 in his past two preseason games.
Dwyane Wade did not play against the Nets, missing his third game this preseason. Wade's pattern of inactivity suggests Saturday's game against the Spurs at AmericanAirlines Arena will be his final action of the preseason. He likely will not play Wednesday in New Orleans and chances are good that he also will skip the final game of the preseason against the Nets next Friday in Miami.
Although the Heat mostly went through the motions against the Nets on Kidd's big night of the preseason, Pierce at least made an effort to physically express his disapproval with comments James made on Wednesday about Pierce and Kevin Garnett leaving Boston for Brooklyn. Pierce slammed into James in the first quarter to prevent a transition bucket and delivered another hard shot to James in the third quarter.
On Wednesday, James suggested Pierce and Garnett were hypocrites for leaving Boston one year after Pierce and Garnett were critical of Ray Allen leaving Boston for the Heat.
"The first thing that I thought was like, 'Wow, Ray got killed for leaving Boston, and now these guys are leaving Boston,' " James said. "I think it's OK. I don't mind it, but there were a couple guys that basically [criticized] Ray for leaving and everybody else is leaving."
James and Chris Bosh led the Heat with 16 points each and were the only players for the Heat to score in double digits. Chalmers had nine points. Center Brook Lopez led the Nets with 14 points.
Sitting out
In addition to Wade, the following regular-rotation players did not play for the Heat: Udonis Haslem, Chris Andersen, Rashard Lewis, who the team said is away for personal reasons, Michael Beasley and Ray Allen. Andrei Kirilenko, Deron Williams and Jason Terry did not play for the Nets.
No setback for Oden
Although Greg Oden said on Wednesday that he was disappointed his left knee responded poorly to his first five-on-five practice, Heat coach Erik Spoelstra refused to call the swelling a step backward in Oden's attempt at a comeback.
"Actually he's making a lot of progress," Spoelstra said. "The fact that he's out here with our guys at this time is ahead of schedule, but we want to temper those expectations. We're thinking big picture with this and we're going to move very patiently."


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Words containing aekwondos
Found 1 words containing aekwondos. Browse our Scrabble Word Finder, Words With Friends cheat dictionary, and WordHub word solver to find words that contain aekwondos. Or use our Unscramble word solver to find your best possible play! Related: Words that end in aekwondos
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You smoke because you're a smokeaholic
Video discusses the reason people smoke is largely based on the simple fact that they are nicotine addicts.
You Smoke Because You're A Smoke-a-holic!
Some smokers say they smoke because they are nervous. Others say they smoke to celebrate. Some think they smoke for energy. Many smoke to look sexy. Yet others smoke to stay awake or to sleep. Some think they smoke to think. One truly unique smoker once told me she smoked to breathe better. Another once said she returned to smoking when experiencing chest pains. She figured the fear of a heart attack is enough to make anyone smoke. None of these reasons satisfactorily explains why people continue smoking. However, the answer is, in fact, quite simple. Smokers smoke cigarettes because they are smokers. More precisely, smokers smoke cigarettes because they are smoke-a-holics.
A smoke-a-holic, like any other drug addict, has become hooked on a chemical substance. In the cigarette smoker's case, nicotine is the culprit. He is at the point where the failure to maintain a minimum level of nicotine in his blood stream leads to the nicotine abstinence syndrome, otherwise known as drug withdrawal. Anything that makes him lose nicotine makes him smoke.
This concept explains why so many smokers feel they smoke under stress. Stress has a physiological effect on the body which makes the urine acidic. Whenever the urine becomes acidic, the body excretes nicotine at an accelerated rate. Thus, when a smoker encounters a stressful situation he loses nicotine and goes into drug withdrawal. Most smokers feel that when they are nervous or upset cigarettes help calm them down. The calming effect, however, is not relief from the emotional strain of the situation, but actually the effect of replenishing the nicotine supply and ending the withdrawal. It is easy to understand why smokers without this basic knowledge of stress and its nicotine effect are afraid to give up smoking. They feel that they will be giving up a very effective stress management technique. But once they give up smoking for a short period of time, they will become calmer, even under stress, than when they were smokers.
The explanation of how physiological changes in the body make smokers smoke is difficult for some smokers to believe. But nearly all smokers can easily relate to other situations which also alter the excretion rate of nicotine. Ask a smoker what happens to their smoking consumption after drinking alcohol, and you can be sure they will answer that it goes up. If asked how much their consumption rises, they will normally reply that it doubles or even triples when drinking. They usually are convinced that this happens because everyone around them is smoking. But if they think back to a time when they were the only smoker in the room, they will realize that drinking still caused them to smoke more. Alcohol consumption results in the same physiological effect as stress – acidification of the urine. The nicotine level drops dramatically, and the smoker must light one cigarette after another or suffer drug withdrawal.
It is important for smokers considering quitting to understand these concepts because once they truly understand why they smoke they will be able to more fully appreciate how much more simple their life will become as an ex-smoker.
Once the smoker stops, nicotine will begin to leave his or her body and within two weeks all the nicotine will be gone. Once the nicotine is totally out of the body, all withdrawal will cease. No longer will they experience drug withdrawal states whenever encountering stress, drinking, or just going too long without smoking. In short, they will soon realize that all the benefits they thought they derived from smoking were false effects. They did not need to smoke to deal with stress, or to drink, socialize, or work. Everything they did as a smoker they can do as a non-smoker, and in most cases they will now do these activities more efficiently and feel better during them.
They will become a more independent people. It is a good feeling and a major accomplishment to break free from this addiction. But no matter how long they are off smoking and how confident they feel, the ex-smoker must always remember that he or she is a smoke-a-holic.
Being a smoke-a-holic means that as long as they don't take a single drag off a cigarette, cigar or pipe, or chew tobacco, or inject it into their bloodstream with a syringe, they will never again become hooked on nicotine. If, on the other hand, they do make the tragic mistake of experimenting with any nicotine product, they will reinforce their addiction. This will result either in returning to their old level of consumption or experiencing a full fledged withdrawal process. Neither situation is fun to go through.
So, once off of smoking, the ex-smoker must always remember just who and what he is – a smoke-a-holic for the rest of his life. Remembering this, you can remain truly independent from nicotine by following one simple practice – Never Take Another Puff!
Related articles:
- I Smoke Because I Like Smoking!
- I smoke because I like the flavor
- I Smoke Because I'm Self-Destructive!
- Were you addicted
- Are you a nicotine junkie?


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Fly into NYC with Ease
Minutes from JFK International Airport with free shuttle service
Welcome to our non-smoking Howard Johnson Inn Jamaica JFK Airport NYC hotel. We are conveniently located off the Belt Parkway with easy access to the excitement of New York City. Our Jamaica hotel is minutes from John F. Kennedy International Airport (JFK) and we offer free shuttle service to and from the airport. Experience The Big Apple and its many delights, and stay happy at our hotel, your home base in NYC.


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RICHMOND, Ky. (AP) Ian Clark scored 21 points as Belmont shot a blistering 67.4 percent, including 73.3 percent from beyond the arc, to defeat Eastern Kentucky 85-74 Saturday night and remain undefeated in Ohio Valley Conference play.
Despite the Bruins' efficiency, hitting 31 of 46 field goals and 11 of 15 from beyond the arc, they saw the Colonels rally from a 15-point deficit to get to 75-71 with 2:54 left.
Kerron Johnson answered with an end-of-shot-clock 3-pointer, Clark added a layup and J.J. Mann hit 3 of 4 free throws to send Eastern Kentucky to its first home loss of the season. Mann finished with 18 points and Johnson 16.
Belmont (17-4, 8-0) used a 13-0 spurt and 16-of-23 shooting - including 7 of 8 from long range - to lead 42-31 at the break.
Corey Walden scored 24 point to lead Eastern Kentucky (16-5, 6-2), which made 11 of 21 3-pointers.


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Kentucky city to build discounted 'socialist gas stations'
Greg Newkirk, Roadtrippers.com
6:02 PM, Jun 21, 2014
With gas prices steadily making their way into the $4 range this summer, it's no surprise that people are getting fed up with how light their wallets feel after fueling up. One Kentucky town has decided to tackle the problem in an unexpected way: with socialism.
Thanks to a pricing feud between fuel distributors and town officials, gas prices in Somerset, Kentucky, have been averaging about $3.74 a gallon, a price that residents are saying can be a solid fifty cents more expensive than most surrounding areas.
The city's fueling depot, which is sourced from a local refinery and services all city vehicles, sells its gas for just $3.10. Now Somerset aims to pass their savings on to locals by creating a city-owned fuel depot in the middle of town.
Their goal? To undercut private sellers and drive down prices through heavy competition.
"We will have 10 different nozzles in a kiosk," the town's economic development business coordinator, George Wilson, told the Commonwealth Journal. "An attendant will be on duty, probably from 8 a.m. to 4 p.m., maybe later, to allow customers to pay by cash or credit card. Gasoline will be available at the pumps 24 hours a day, seven days a week, using credit cards."
Somerset is currently working on installing the new pumps, which will give buyers discounted gas calculated by averaging gas prices from towns within a 50-mile radius. Officials hope to have the new station up and running this summer.
If you're going to pay a premium for your gasoline, you might as well enjoy fueling up. Check out the Roadtrippers.com guide to the coolest gas stations across America, where the photo ops are totally worth a little extra at the pump.


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Customer Sales Advisor - Wythenshawe
- Recruiter
- Location
- Altrincham
- Salary
- £15,000 - £19,999
- Posted
- 03 Apr 2018
- Closes
- 19 Apr 2018
- Category
- Retail and Sales
- Contract Type
- Permanent
- Hours
- Full Time
Customer Sales Advisor
Salary £17,500 + £9,000 OTE (uncapped) + brilliant benefits
Shift Pattern: Monday to Sunday, 37.5 hours per week, shifts between 8am and 8pm
Join Vodafone as a Customer Sales Advisor for the chance to earn uncapped commission. You'll be provided with the training, development and support to give you every chance of earning brilliant rewards, with typical on-target earnings of £26,500 but the chance to earn much more. We'll also give you a great benefits package and a vibrant work environment that will soon feel like home - this is a fantastic opportunity you won't want to miss.
In this dynamic and varied role, expect to be handling all sorts of queries from customers who are coming towards the end of their contract who may be looking to cancel or upgrade. It will be your role to get to the bottom of any issues the customer may have, ensuring they remain connected to products and services that best suit their needs.
If you've worked as a Sales Assistant, Sales Executive or Telemarketer before that would be great, but previous experience is not necessary. You just need an excellent telephone manner and great communication skills. The ability to listen and ask the right questions is really important, so you understand what's driving the customer to potentially look elsewhere. Resilience, determination, empathy and the rapport to quickly develop a relationship with any customer are key traits that you'll need to provide excellent service. Regardless of your background this role is all about your personality and behaviours - we'll provide you with all the training that you need.
We are a global company but a close-knit team that gets a real buzz from working together. We invest in a three-week training period so that you get to really know your colleagues and the standards we set before helping our customers with their queries and problems. Close support is provided, tailored for our people's needs. Team leaders and management are really approachable and always on hand to help with any problem or situation.
As well as brilliant earning potential we offer a benefits package that includes:
- 33 days' holiday (including 8 bank holidays) and the option to buy or sell an additional 5 days
- Up to 80% discounted SIM only and Broadband packages for yourself, your family and friends
- Onsite gym
- Onsite barista style coffee shop
- Discounts at 100s of high street stores
- World leading maternity package
- Free health assessments to support your well-being
- Plus many more


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Follow RFF News on your cell phone. Text follow rff to 40404 you will receive txt messages with RFF News
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Next week's episode is sure to be a NEL.And O M G, what an episode! I seriously thought Claire and Michelle was going to be eliminated the whole time. But in the end, it was a fight for last place for both the all-male teams, who should have been the strongest competitor!And this is why i like TARA. It is so random as always. Who would have thought Ethan and Khairie will be eliminated?? Especially with the Richards falling SO FAR BEHIND.Guess the Detour was not really a balanced one, but the Roadblock, is OMG.... HARD??I'm so glad that the top 3 teams this leg are the teams that i really want to see. And guys, you just gotta admit that TARA loves all-female team. For the FOURTH season in a row, we're going to have a all-female team in the final three. And for the first season ever, we might even have two all-female teams in the final 3, as it seems as though Natasha and Hussein can get eliminated anytime if they made a bad decision. But seriously, these two are lucky that a TBC saved their asses! But i love to watch them anyway!Although the editing was bad, but dramas between the teams made it really exciting. Claire and Michelle in the F4 along with Natasha and Hussein and Jess and Lani.. who would have thought?? I think the Richards might win this.. but you really never know. Let's hope somehow the Marc and Rovilson might find their way to them. *evil*And the malaysian boys eliminated?? I thought Claire and Michelle might share the password with the boys at the Roadblock. But i guess they didn't want to take the chance. But then again, people will hate the girls and the malaysian boys over the Richards elimination.. so i guess i'm glad they didn't do it. But you gotta agree that the Richards are much more stronger than Ethan and Khairie and it's better to keep the Malaysian boys around..OVERALL, this has been a fairly exciting leg!! Not to forget for the third season in a row, we have a team in the Final 4!! GO SINGAPORE!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
Ruth, the last leg in Australia was not NEL. You have to have a real pit stop for that it; it was only a "virtual pit stop." What it was is a To Be Continued (TBC) leg similar to several that have occurred in the history of the U.S. Amazing Race:AR6, ep. 6 HungaryAR7, ep. 8 IndiaAR8, ep. 11 Canada, with a small amount of the U.S.on each endAR10, ep. 9 AustraliaAR14, ep. 10 Beijing China
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colonic(redirected from colonic bands)
Also found in: Dictionary, Thesaurus.
Related to colonic bands: Haustra, Epiploic appendages
Relating to the colon.
colonic/co·lon·ic/ (ko-lon´ik)
1. pertaining to the colon.
See colon.
colonicadjective Referring to the colon.
noun See Colon therapy.
Relating to the colon.
colonic (k·lˑ·nik),
adj pertaining to the colon.
colonic (k·lˑ·nik),
n irrigation of colon for cleansing by injecting large amounts of fluid.
pertaining to or arising from the colon.
colonic aganglionosis
see colonic aganglionosis.
colonic atony
see megacolon.
colonic atresia
affected neonates appear normal at birth but develop abdominal distention quickly. No feces are passed and death occurs at about one week unless surgical repair is effected. The defect occurs sporadically in most species. In horses and cattle it can be inherited, in cattle it can result from over-vigorous palpation of the fetus between 35 and 41 days gestation at pregnancy diagnosis, but the cause is not determined in most cases.
colonic bands
see teniacoli.
colonic constriction
due to contraction of peritoneal adhesions in horses; causes chronic or intermittent colic.
colonic contraction
permanent inability of colon to dilate due to congenital aganglionosis.
colonic crypt
straight tubular glands in the colonic mucosa.
colonic entrapment
see left colonic displacement (below).
colonic foreign body
foreign bodies, e.g. halter shanks, are found in the colon in horses, having passed the gastric sphincter and the ileocecal valve; quickly encrusted with salts.
colonic impaction
intractable constipation occurs in dogs and cats, primarily with obstruction by foreign material and secondarily when there is an obstruction to the normal passage of feces, including retention because of pain at defecation. See also colon impaction colic of horses.
colonic infarction
see thromboembolic colic.
colonic ischemia
deprivation of blood supply to all or part of the colon. See also intestinal torsion, intestinal strangulation, intestinal obstruction colic.
colonic obstruction
see colonic impaction (above), intestinal obstruction.
right dorsal colonic displacement
displacement of the right dorsal colon in the horse to the area between the right body wall and the cecum, in an anterior direction so that the pelvic flexure comes to lie against the diaphragm.
colonic rupture
occurs, apparently spontaneously, in mares at foaling, death occurring soon afterwards.
colonic torsion
in horses, see under equine colic. In cattle, torsion of the coiled colon is an acute obstruction with coils of gas-distended colon visible in the right flank and palpable per rectum.
colonic wash
fecal samples can be collected from reptiles by flushing the colon with saline through a catheter inserted through the cloaca and into the colon.
Patient discussion about colonic
Q. How is colon cancer diagnosed?
A. thank you lamsophie, great answer...
Q. how successful is the treatment of removing the colon? Are there any people who have had their colons removed successfullly? What other treatment options are there and how successful are they?
A. colon removal is a treatment for various situation, usually a last resort treatment...when anything else just wouldn't or couldn't work.
it's "success" as a treatment depends on the cause. i can tell you that this is the area that absorbs B12 and bile and most of the water, so expect a shortage of that three. in the water and bile case- expect watery stool...
sorry all that doesn't seem such a nice state but when Dr. come to the point they have to do it- there must be a good enough reason.
it's "success" as a treatment depends on the cause. i can tell you that this is the area that absorbs B12 and bile and most of the water, so expect a shortage of that three. in the water and bile case- expect watery stool...
sorry all that doesn't seem such a nice state but when Dr. come to the point they have to do it- there must be a good enough reason.
Q. Is colon cancer hereditary? My uncle died of colon cancer and as I've been having some unexplained problems these days- of vomiting etc I'm really afraid I may have it as well. Is it hereditary? What are the first symptoms?
A. Thank you Bianca for your answers! helped a lot...this is a great site!More discussions about colonic


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Applications of Digital Image Processing, Issue 15
Society of Photo-optical Instrumentation Engineers, 1993 - Image processing
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Nonlinear techniques for the enhancement of images reconstructed from projections 177103
Intelligentbased optical Chinese character recognition system by using integrated character
Segmentation of aneurysms via connectivity from MRA brain data 177106
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WINNIPEG - Hudson the polar bear's celebrity power was immediately evident when he took his first public romp around his enclosure at the Assiniboine Park Zoo.
"There he is! There he is!" someone observed Thursday as a furry head with a charcoal nose, two button eyes and cupcake ears poked out of his den.
The 15-month-old bruin then squeezed his rather broad backside through the opening and lumbered toward a clapping crowd that cheered "hip, hip, hooray!"
He cemented his star status by sliding down a small embankment on his big pigeon-toed paws. A ramble over to the fence where he stood on his hind legs to show off his formidable bulk — already at 185 kilograms — sealed the deal.
"Only 15 months old!" someone exclaimed.
Hudson is the first inhabitant of the Winnipeg zoo's International Polar Bear Conservation Centre. The outdoor part of the exhibit has been renovated and expanded specifically to accommodate polar bears and is three times its original size. The hope is that the centre will be a world-class attraction for tourists and researchers.
"The polar bear is a sentinel species with respect to climate change," said Manitoba Premier Greg Selinger before Hudson was introduced. "As goes the polar bear, so goes the planet.
"And as the polar bear becomes more threatened ... it means we have to be very vigilant to protect our planet from climate change. The exhibit here gives a very good illustration of what's happening around the world these days."
Selinger predicted the exhibit will attract attention for three reasons.
"We are the global centre for polar bear conservation, education and research," he said. "This facility here will allow families from Winnipeg and tourists from around the world to come and see Hudson and other threatened members of the polar bear species, to learn about climate change."
The $4.5-million centre will also provide a home for orphaned, injured or problem bears.
Hudson is adapting well after arriving on a cargo jet two weeks ago from the Toronto zoo, where he was born, said Tim Sinclair-Smith, director of zoological operations in Winnipeg.
Hudson's bio on the zoo's website says he enjoys icy snacks filled with fish or vegetables and rolling around in snow or mud.
Sinclair-Smith said Hudson is friendly, likes people and loves Winnipeg's frosty winter conditions.
That was certainly evident Thursday as the bear frolicked in front of well-wishers, batted at a red ball and chowed down on treats.
Hudson has big pawprints to fill.
He replaces the zoo's beloved Debby, who was recognized by Guinness World Records as the world's oldest polar bear when she died in November 2008. She was 42 — almost twice the age her kind usually reach in the wild.
Hundreds of people turned out for Debby's memorial and the zoo received countless letters from around the world.
The big white bear's statue, donated by the province of Manitoba, stands in her honour at the zoo.
Debby was not forgotten on Thursday. Winnipeg Mayor Sam Katz made sure of that.
"I know many of you remember one of the treasures that we had here not that long ago back in 2008, Debby," he said. "It's been five long years."
Note to readers: This is a corrected story. A previous version said Greg Selinger was Winnipeg mayor.


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caught in the early mornin rays of the sun...
(before the neighbors can see me hopping around in the wet grass, in my jammies).
THAT LAST SHOT especially dearest, is remarkable...to catch the fine lines of the web, what a gift. Ohhh, what a lovely summer this has been! Anita
The spider web shot is amazing!!! Have a beautiful day!!!
Thanks for the link to the shop in Franklin!!!
Oh my! The photo of the web is amazing! Love it. I'm not blog surfing much these days, but I always visit you!
I think your pajama shots are fabulous.
Good morning, Erin! :) Your spiderweb shot is fabUlous.
Why is it our best photos are taken in our pjs?
Thanks for stopping by... I'm loving these Garden photos, especially the one with the Spider Web... Spiders are such Natural Artists aren't they, I've always been intrigued by their Web designs and how delicate yet strong they are. The Phlox are beautiful, I could envision a large Bouquet of them in a Rustic Jug on the Breakfast Table...
Dawn... The Bohemian
I run around in my garden in my jammies too. Is there any better way.
I love phlox but I seem to be riping mine out these days. Yours are lovely though.
had a wonderful morning walk in your garden with a cup of kona coffee, tx for sharing the tour :)
The spider web shot is just beautiful!!
Gorgeous, gorgeous!!!! A dreamy summer morning, I just love these images. That last one with the web is like an impressionist painting. :)
– g
ooooh, dare we form a hopping around the garden in jammies group? sign me up. guilty as charged! love the phlox.
Oh yes it is...Oh YES it is. Stunning images. Your soul speaks through your lens. Beautifully!
Wow Erin!!!
Gorgeous gorgeous! love the phlox! (had no idea that is what it was called!)
Beautiful photos!
Oh, and thank you for looking for the name of the fabric. You are too sweet but I hope it hasn't been a pain. Don't worry if you can't find it. Thank you again!


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Does God Desire All to Be Saved? (New Book from John Piper)
This extended excerpt is from the introduction to John Piper's Does God Desire All to Be Saved? — now available in paperback and as a free PDF from Crossway and Desiring God.
When climbing from lowlands to mountaintops, one must often pass through clouds. That's the way it has been for me all my life as I have tried to get the best views of the glory of God.
When you enter a layer of clouds, it helps to have a guide to keep you away from the precipices and on the path to the other side of the murkiness. That's one way to view this book. I hope it will serve as a guide upward through the haze and confusion about God's electing and saving will.
Steep Paths and Thick Clouds
I admit that some of the paths in this book are steep. And some of the steepest places are through the thickest clouds. The climb is not for everyone. We all have different gifts, and not everyone is called to this kind of intellectual climb. I don't mean that the non-climbers will see less glory or worship with less passion. There are glories in the valleys. And there are paths into beauties of God that are less intellectual. I would not dare to claim that those who do this sort of climbing always see or savor more glory than those with wider eyes for the glory that is right there in the meadow.
Nevertheless, some of us are wired to make this climb. There is not much choice in it. We should no more boast about doing it than one should boast about being a morning person. Almost every time we open our Bibles, we see challenges. Puzzles. Mysteries. Paradoxes. Mountain paths beckon us, but seem to lead in opposite directions. We move toward these paths like bumblebees toward morning glories.
So if you are like me in this way, I would like to invite you to take a climb. I don't claim to be superior, but it may be that on this mountain I've gone up and down enough times to be of some help. There are clouds. It can get really murky on the way to the brightness on the other side. I would like to help if I can.
Two Paths Beckon
The paths that beckon us on this mountain are the path of God's election and the path of God's will for all people to be saved. Election seems to say that God has a people who are his, and he sees to it that they come to Christ and are saved. But the other path seems to say that God loves everyone and invites everyone to come, and wants them all to be saved.
On the mountain path of election, Jesus says, "I have manifested your name to the people whom you gave me out of the world. Yours they were, and you gave them to me, and they have kept your word" (John 17:6). And in another place, he says, "No one can come to me unless it is granted him by the Father" (John 6:65). Or, as God says in Romans 9:15, "I will have mercy on whom I have mercy, and I will have compassion on whom I have compassion." Then Paul draws out the inference: "So then it depends not on human will or exertion, but on God, who has mercy" (Romans 9:16).
But on the mountain path of God's desire for all, Jesus says to the city that is about to kill him: "O Jerusalem, Jerusalem, the city that kills the prophets and stones those who are sent to it! How often would I have gathered your children together as a hen gathers her brood under her wings, and you were not willing!" (Luke 13:34). And he offers an open and free invitation to everyone who is heavy laden, thirsty, and perishing: "Come to me, all who labor and are heavy laden, and I will give you rest" (Matthew 11:28); "If anyone thirsts, let him come to me and drink" (John 7:37); "For God so loved the world, that he gave his only Son, that whoever believes in him should not perish but have eternal life" (John 3:16).
It's an old paradox. I didn't climb this mountain by myself. More seasoned climbers than I helped me along the way. I'll introduce them to you as we go.
For the Love of God and People
The aim of the climb is not intellectual satisfaction. The aim is worship. God gets more honor when we worship on the basis of what we know about him than he gets if we worship on the basis of what we don't know. If our effort to know God more clearly is not an effort to love him more dearly, it will be fatal. "Knowledge puffs up, but love builds up" (1 Corinthians 8:1). This means that the only knowledge worth having in the end is knowledge that leads to love — love for God and love for people.


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What Lurks in the Dark Shadows of the Heart? Most men who see Amanda Charles dance worship her from afar. But one "admirer" is getting too close to the glamorous showgirl...perilously close. The looks, style, and grace that make Amanda sparkle on stage have now made her something else: the target of a serial killer. Detective Tristan MacLaughlin has drawn the case that is pulling him deep into a world of pageantry and dazzle -- and closer to the stunning, violet-eyed beauty who keeps her secrets and emotions locked tightly inside. Tristan never thought he'd be bewitched by Amanda's charms, never though he'd care. And he never imagined that by wanting her, by trying to protect her, he'd place her in the gravest danger of all.
2018 Download PDF/ePUB eBook - comic-bookmania.com


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A colleague who was looking to refinance his mortgage to today's record low interest rates, just told me that he opted instead for a "modification."
No, he's not behind on his monthly payments, nor even struggling to make them. He is current, and modifications on current loans have been around for years, though they are increasingly rare and consequently few people know about them.
My colleague's bank, New Jersey-based First Niagara, allowed him to do the modification to a lower interest rate. Here's the best part: My colleague had to pay just $500 for his 7/1 Adjustable Rate Mortgage (ARM) to go from 4 percent to 3.125 percent, which is an eighth of a percentage point above the going rate, but still a great move. No principal payment, no new underwriting, no nothing.
I called First Niagara and asked if they do mortgage recasts. The first agent said yes, but when I asked to be transferred to a mortgage specialist, and then identified myself as a reporter, I was disconnected … three times.


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Taylor Swift and Harry Styles may be an item again
The celebrity media obsession with Taylor Swift's love life continues, with the latest reports linking her with an earlier crush, Harry Styles, teen heartthrob of British boy band One Direction.
The celebrity media obsession with Taylor Swift's love life continues: The latest reports link her with an earlier crush, Harry Styles, teen heartthrob of the British boy band One Direction, currently fluttering tween hearts everywhere.
The breathless news, from MTV and others, is that Swift, 22, was spotted holding hands with Styles, 18, on the X Factor set, where she was rehearsing her Thursday night performance of a song from her latest record, Red.
X Factor host Mario Lopez spilled the beans about what he saw on set during his radio show on 104.3 MyFM on Friday night.
"Taylor Swift was the special guest performer, and a little inside scoop for you: During rehearsals, Harry from One Direction came and slapped me on the back and said, 'Hey, Mario, how ya doing?' And I said, 'What are you doing here?' And he sort of [pointed] toward Taylor."
Swift and Styles walked away "hand in hand," Lopez reported.
Guess Swift must be getting over the end of her summer fling with Kennedy scion Conor Kennedy, also just 18. So far, no word on whether she's written a song to commemorate that broken romance.
According to MTV, Swift and Styles met last spring at the Kids Choice Awards. Hollywood Life reported that in April they had a brief fling, which was suspended when he went on tour to Australia and she found out he was messing around with other girls.
It may be that I Knew You Were Trouble is a song about Styles, a boy who brings new meaning to the phrase "big hair." He also has a history of dating older women: One of his romances was with a British woman 14 years older.
But one thing is certain: If they are dating again, the publicity can only be good for both of them — she with already-top-selling Red to promote, and he with 1D's sophmore record, Take Me Home, to support.
True love.


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Welcome to The Halloween Connection
We are the best and top-rated Halloween store on the web, with a wide selection of Halloween costumes, accessories, decorations and more! We specialize in movie props and graveyard props, including tombstones, Gothic décor, skeletons, zombies and Animatronics, as well as distinctive haunted house supplies, for your professional or home haunt. We have bloody zombies, gory props, Halloween coffins, scary clowns, Halloween lighting and much more! Check out our Halloween costumes, including sexy costumes, plus size costumes and gory and bloody make-up. Find great deals in our Halloween Clearance section. Choose Halloween Connection for all your horror and Halloween needs. We take pride in the quality of our products and our excellent customer service. If you don't see what you're looking for on our website, please contact us and we will do our best to locate or make it for you! All of the products we manufacture can be ordered in 3D paint, simply by calling us at (702) 682-6677 or (702) 493-8912 after placing your order to request this option.