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Protect the ocean and you protect yourself. ~Jean-Michel Cousteau
Protect the ocean and you protect yourself. ~Jean-Michel Cousteau
Beluga whales are sometimes called canaries of the sea because they seem to sing…
There are places on this planet where it's a marvel that anything survives. But in the cold Arctic waters of the far north, the sea is alive with sound. The canaries of the sea are singing. They're beluga whales, named from the Russian word for "white ones." They're an evolutionary surprise—a warm-blooded mammal in a numbingly cold sea. Resembling curious ghosts, these intelligent mammals use one of the most complex sonars of any animal.
Belugas inhabiting Cook Inlet, close to Anchorage, were added to the list of endangered species in October 2008. The National Oceanic and Atmospheric Administration stated that a decade-long recovery program had failed to ensure the whales' survival. The relationship between people and belugas is ancient. For more than 4,000 years, hunters of the north have depended on these whales for their own survival in a land with little to offer. These traditional cultures have now partnered with scientists and modern technology to protect the beluga, which in turn, ensures their own future. Read the rest of this article on the I Count for myEARTH blog.
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Boomers vs. Millennials: Will We stay the Course or Change It? Instead of Sabotaging another Relationship, here's how to Run into your Fear. Join: Elephant's Fall 2016 Academy. I Hope he Loves you like This. {Poem} How Sleeping with the Lights on (Literally) Changes our World. When you're Stuck, Remember to ask yourself this Question. To the Man who Ruined me.


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Ecto-5′-nucleotidase promotes invasion, migration and adhesion of human breast cancer cells
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DOI: 10.1007/s00432-007-0292-z
- Cite this article as:
- Wang, L., Zhou, X., Zhou, T. et al. J Cancer Res Clin Oncol (2008) 134: 365. doi:10.1007/s00432-007-0292-z
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Associated with many molecules, metastasis includes cell adhesion to extracellular matrix, migration towards specific direction and invasion into local vessel of distant organs. The purpose of the present study was to evaluate the role of ecto-5′-nucleotidase (eN, ecto-5-NT, CD73) generated extracellular adenosine in biologically malignant behaviors of human breast cancer cell lines.
Materials and methods
Two human breast cancer cell lines, T-47D with lower expression of CD73 and MB-MDA-231 with higher expression of CD73, were used to investigate the functions of CD73. The effects of CD73 over-expression on invasion, migration and adhesion were observed in T-47D transfected with pcDNA-NT5E plasmid. The effects of specific CD73 inhibitor, α, ß-methylene ADP (APCP), were observed in MB-MDA-231 cells.
The results showed CD-73 overexpression increased invasion, migration and adhesion to ECM of the pcDNA-NT5E transfected T-47D cells compared to the saline and mock vector controls. The increased cell mobility of CD-73-overexpressed T-47D cells was blocked by APCP. Adenosine increased the mobility of wild type T-47D cells. APCP inhibited the mobility of the MB-MDA-231 cells.
Taken together, our results indicated that CD73 may facilitate the adhesion, migration and invasion of human breast cancer cells through its enzyme activity of generating adenosine. This study provided a possibly molecular mechanism of metastasis of breast carcinoma.
KeywordsCD73AdenosineBreast cancerInvasionAdhesionMigration
Recent data suggested that approximately 90% of cancer deaths were attributable to the presence of distant metastasis (Hanahan and Weinberg 2000). Therefore, treatments directly targeting metastatic disease by specifically inhibiting the invasive behavior of tumor cells are extremely attractive. Such approaches might increase complete remission rate by limiting the tumors to original location for radiation and surgical treatments. Acquisition of metastatic capability might be an early event during tumorigenesis and invasive growth because their underlying molecular pathways and abnormal cell behaviors are a prerequisite for the establishment, growth and survival of the primary tumour (Elvin and Garner 2005). The process of tumor metastasis includes multiple steps such as adhesion, migration and invasion.
Statistical data showed that in developed countries, breast cancer is the most common tumor in women, and it is the most considerable cause of cancer-associated morbidity and mortality. Over the last few years, new molecular markers have been discovered as significant targets for diagnosis and therapy. One goal is to identify a specific molecular assay for each breast tumor and then to obtain an individualized therapeutic approach. Today, a variety of molecular factors are being evaluated for potential application in clinical practice to predict the prognosis and response to therapy of breast cancer patients. Expression of CD73 in breast cancer cells may become of the candidates.
CD73, known as ecto-5′-nucleotidase (5′-NT, EC 18.104.22.168), is a glycosyl-phosphatidylinositol-linked 70-kDa molecule. Its enzymatic activity involves catalyzing the dephosphorylation of ribo- and deoxyribonucleotide 5′-monophosphates to their corresponding nucleosides. While the enzyme has broad substrate specificity, it prefers purine ribonucleoside monophosphates. Adenosine 5′-monophosphate (5′-AMP) is the best substrate. CD73 was defined as the lymphocyte differentiation antigen CD73. This surface antigen may regulate the availability of adenosine for the interaction with the cell surface adenosine receptor by converting 5′-AMP to adenosine. CD73 was highly expressed in many human solid tumors, and the level of its expression was associated with tumor neovascularization, invasiveness, and metastasis and with shorter patient survival time (Spychala 2000). In a survey of gene expression among a range of human carcinomas, CD73 was found to be upregulated in carcinomas including those of colon, lung, pancreas and ovary (Su et al. 2001). Recent study suggested that high expression of CD73 may be associated with papillary thyroid carcinoma. Clinically, the strong expression of CD73 in papillary thyroid carcinomas could be a diagnostic aid in the differential diagnosis of thyroid tumors (Kondo et al. 2006). Using CD73 (1E9.28.1) monoclonal antibody, CD73 expressions were observed in breast carcinoma. Moreover, Spychala et al. showed that CD73 was negatively regulated by estrogen receptor-α(ER-α) in a dominant fashion and suggested that CD73 expression and its generation of adenosine may relate to breast cancer progression. Increased expression of CD73 in a subset of ER-negative cells may serve as a novel marker for more aggressive breast carcinoma (Spychala et al. 2004).
Adenosine, a purine nucleoside, acts as a regulatory molecule and is a ubiquitous nucleoside present in all body cells, by binding to specific G-protein-coupled A1, A2A, A2B, and A3 cell surface receptors. Adenosine is a natural metabolite that plays a role in several physiological and pathological processes such as inhibition of platelet aggregation, cardioprotection after ischemia, vasodilation, mast cell activation, and lypolysis. Adenosine may interfere with neutrophil's adherence to endothelial cells. Adenosine and A2A agonists reduce inflammation in part by reducing adhesion molecule expression and neutrophil adherence to endothelial cells (Okusa et al. 2000). Adenosine also induces a differential effect on tumor and normal cells. Adenosine and AR agonists were recently shown to inhibit growth or induce apoptosis in several types of cancer cells. While inhibiting in vitro melanoma, leukemia and lymphoma cell growth, it stimulated bone marrow cell proliferation. This dual activity was mediated through the A3 adenosine receptor (Fishman et al. 2001; Lu et al. 2003). Adenosine was also able to promote tumor cell migration, stimulate angiogenesis, increase intratumoral blood flow and confer protection against radiotherapy. Adenosine is generated by CD73 enzyme activity. Although adenosine exerts a number of effects that promote tumor expansion (Mujoomdar et al. 2004), the role of CD73 enzyme activity in adhesion, migration and invasion of breast cancer cell lines is unknown. Considering the different effects of adenosine in diverse normal tissues and malignant tumors due to variable distribution of its receptors, we observed the role of adenosine in breast cancer cells, to see whether it promotes tumor cell invasion, migration and adhesion or not. In this study, we developed a transient gene transfection technique in T-47D breast cancer cells which express low levels of CD73 before transfection to investigate the function of CD73. The effects of adenosine on invasion, migration and adhesion in wild type human breast cancer cell T-47D were investigated by pre-treating with adenosine. The concept was further proved in MB-MDA-231 breast cancer cells, which express high level of CD73, using CD73 specific inhibitor.
Materials and methods
Cell lines and culture conditions
Breast carcinoma cell lines T-47D and MB-MDA-231 were obtained from the American Type Culture Collection (ATCC, Manassas, VA). MB-MDA-231 cells were routinely maintained in Dulbecco's modified Eagle's medium (Gibco, CA, USA) supplemented 10% fetal bovine serum at 37°C in a humidified atmosphere of 5% CO2 and 95% air, penicillin (50 units/ml), streptomycin (50 units/ml) (all of these from Life Technologies, Inc., Burlington, Canada). T-47D human breast cancer cell line was incubated in the same medium plus 0.2 U/ml insulin (Life Technologies, Inc., Burlington, Canada). Cells were split two or three times weekly at a ratio between 1:4 and 1:8.
pcDNA-NT5E plasmid construction and transfection into T-47D cells
To determine the possible role of CD73 in invasion, migration and adhesion of human breast cancer cell lines in vitro, CD73 gene was cut from the vector pBluescript SK(±), then cloned into the unique KpnI and BamHI cloning site of the pcDNA3.0 expression vector. The plasmid pcDNA-NT5E was transfected into T-47D cells using Lipofectamine™ 2000 reagent (Invitrogen, Carlsbad CA, USA) following the protocol offered by the manufacturer. Briefly, parent T-47D cells (3 × 105/well) were plated in 6-well plate and incubated at 37°C with 5%CO2 until 90–95% confluence. Pre-mixed lipofection and plasmid DNA in a ratio 6 (μl): 3 (μg) were added to the wells with gentle swirling for 24–48 h. Clones were selected for invasiveness, motility and adhesion analysis after transfection.
Invasion, migration and adhesion assays in transfected T-47D cell line
Invasion assay was done in 8 μm polyethylene terephthalate filter inserts in Boyden chambers (Becton Dickinson, Bedford, MA, USA). The 8 μm pore inserts were coated with 10 μmol/ml of Matrigel (Becton Dickinson Labware, Bedford, MA) 20 μl at 37°C for 30 min. Breast cancer cells containing transfection pcDNA-NT5E T-47D, mock vector and wild T-47D were trypsinized with 0.25% trypsinase, centrifuged at 1,500 g for 5 min, and suspended in serum-free DMEM medium. 200 μl cells at final concentration of 2 × 105 cells/ml were added to coated filters in triplicate wells. The bottom compartments of the chambers contained 750 μl serum-free medium. The cells were incubated at 37°C in a humidified atmosphere of 95% air and 5% CO2 for 48 h. The matrigel coated on the upper surfaces of the filters was mechanically cleaned with cotton swabs. Cells that migrate through the filters to the opposite side of the filters were fixed with 4% paraformaldehyde (for 20 min at 37°C), and then stained with hematoxylin and eosin, photographed, and counted. Under the inverted microscope, cells in five medium power fields (200×) were counted double blindly by two independent examiners.
Migration assay was determined in the same way described as the invasion assay except that the cells were plated on top of uncoated 8 μm pore polyethylene terephthalate filters in the Boyden chambers. In addition, invasion and migration assays were performed in T-47D cells transfected pcDNA-NT5E plasmid after pre-treating with 3 μM/ml of APCP for 1 h.
Cell adhesion assay
Cell adhesion assay was performed with 96-well tissue culture plates. Plates were pre-coated with 16 μg/ml ECM gel (from mouse sarcoma) diluted with phosphate buffered saline (PBS), 100 μl per well for 4 h at room temperature. Non-specific binding sites in the wells were blocked with 1 mg/ml BSA mixed with PBS for 2 h at room temperature. After the cells grew to approximately 80% confluence in DMEM containing 10% FBS, cells were trypsinized, suspended in serum-free medium at a concentration of 2 × 104 cells/ml and applied 100 μl to each well. The cells were incubated under routine condition as above for 2 h. Following incubation, non-adherent cells were removed by carefully washing three times with PBS and gently rocking. Adherent cells were fixed with 3% paraformaldehyde for 10 min, cleaned with PBS three times, air-dried, and stained with 100 μl 0.5% crystal violet for 5 min. The cells were then washed three times with PBS and lysed with 100 μl ethanol of 1% acetic acid solution and read at A570 on multifunctional reader (Tecan GENios, Zurich, Switzerland). Results were expressed as optical density (OD) at 570 nm ± Standard Error (SE) of three independent experiments. The experimental groups include wild T-47D, T-47D transfected mock vector, T-47D transfected pcDNA-NT5E plasmid, and T-47D transfected pcDNA-NT5E plasmid plus APCP treatment.
Invasion, migration and adhesion assays in T-47D cell treated with adenosine
To study the possible effects of adenosine on invasion, migration and adhesion of wild T-47D cells, cells were treated with adenosine at 100 μM. In human epidermoid carcinoma A431 cells, adenosine evoked a biphasic response in which a concentration of 10 μM produced inhibition of colony formation; however, at concentrations up to 100 μM, this inhibition was progressively reversed (Tey et al. 1992). Our preliminary experiment showed that 100 μM adenosine could reverse inhibition of APCP in T-47D cells transfected with pcDNA-NT5E-CD73 (data not shown). The control group was treated with PBS. Invasion, migration and adhesion assays were conducted as previously described.
Invasion, migration and adhesion assays in MB-MDA-231 cell treated with APCP
The aggressive mammary cell line MB-MDA-231 over-expresses CD73. APCP is specific inhibitor of CD73 enzyme activity. Invasion and migration were examined in MB-MDA-231 cell pre-treated with different concentrations of APCP (0, 3, 6, 9 and 12 μM).
Fibronectin and collagen type IV (Sigma, St Louis, MO, USA) were major ECM proteins. Tumorous cells adhere to ECM before invasion. Additionally, earlier reports suggested that CD73 may interact with ECM proteins (Stochaj et al. 1989). It was also demonstrated that laminin and fibronectin, but not collagen, affected CD73 activity in chicken gizzard. The effect of APCP on the adhesion of breast cancer MB-MDA-231 cells to ECM and its major components. Overnight, 96-well culture plates were coated with 100 μl (10 μg/ml) ECM, fibronectin and collagen type IV, respectively. Cell adhesion to these coated surfaces was observed as described previously.
Statistical analysis
Mean values were calculated from data obtained from three or more separate experiments and reported as mean ± SEM. The significance of the difference between groups with multiple comparisons was assessed by one-way analysis of variance (ANOVA). P values < 0.05 were considered statistically significant.
CD73transfection increases T-47D invasion, migration and adhesion to ECM
Adenosine increases T-47D invasion, migration and adhesion to ECM
APCP inhibits invasion, migration and adhesion of MB-MDA-231 cells
Metastasis of breast cancer is the main cause for high mortality. If the metastatic mechanism is well known, the cancer patients could be treated before and after cancer metastasis to increase remission rate. Determining the timing of anti-invasive intervention is challenging because the key pathways determining the invasive behavior of tumors are less well clear than those that regulate tumor proliferation.
CD73 is a membrane-bound glycoprotein, which hydrolyzes extracellular nucleotides into bioactive nucleoside intermediates (Zimmermann 1992). It stimulates vasodilation, angiogenesis and cell growth in cancer (Barcz et al. 1998; Khoo et al. 1996; Lelievre et al. 1998; Natori et al. 1992; Rathbone et al. 1992). However, the relationship of CD73 expression, activity, and adenosine generation to tumor cell biology and tumor progression has not yet been determined. Previously published data suggested increased expression of CD73 in breast cancer when compared with nonmalignant surrounding tissues (Canbolat et al. 1996; Thompson et al. 1992; Ujhazy et al. 1994). Preliminary survey of a panel of cancer cell lines showed a highly variable expression of CD73 (Spychala 2000). More detailed analysis of breast cancer cell lines in previous studies revealed a striking negative correlation of CD73 expression with estrogen receptor (ER) status that in turn might be predictive of a more invasive tumor phenotype, as determined in animal models (Bachmeier et al. 2001; Gilles et al. 1998; Manni et al. 2002; Mullen et al. 1996; Sommers et al. 1994). Although CD73 is highly expressed in many tumor cells including breast cancer, its specific function during tumor metastasis is unclear.
Mammary carcinoma frequently metastasizes to specific organs, including the regional lymph nodes, the lung and bone marrow. Several genes encoding adhesion and matrix proteins were up-regulated in tumors that preferentially metastasize to the lymph node. One of the up-regulated genes in lymph-homing tumors was CD73. The expression of CD73 in lymph node metastases was higher than in the primary tumor (Lee et al. 2003). In tumor progression, CD73 may act as a molecule that directed lymph node specific-metastasis. CD73 has also been suggested to serve as a lymphangiogenic marker in the lymphatic spread of colorectal cancer cells to regional lymph nodes. Therefore, it may have a prognostic value for colon cancer patients (Parr and Jiang 2003). Present data revealed that among different melanoma cells, up-regulated expression of CD73 is associated with a highly invasive phenotype (Sadej et al. 2006). In addition, the level of CD73 activity showed significant correlation with tumor grade and location (Eroglu et al. 2000). Previous studies indicated that the CD73 implicated in cell–cell and cell–matrix interactions as protein (Vogel et al. 1991) and involve in drug resistance and tumor-promoting functions (Ujhazy et al. 1996). CD73 has been shown to have a role in regulating lymphocyte adhesion. Antibodies against CD73 was shown to interfere with lymphocyte adhesion to cultured endothelial cell (Airas et al. 1993, 1995; Arvilommi et al. 1997). However, the exact mechanism of CD73 functions has remained unclear. Some data showed that CD73 functions at the activation step in the multi-step adhesion cascade and promotes lymphocyte binding to endothelial cells via a lymphocyte function-associated antigen-1-dependent mechanism (Airas et al. 2000). Therefore, CD73 is likely an important molecule in breast cancer metastasis.
Kamath et al. (2001) classified MB-MDA-231 as highly invasive breast cancer cells while T-47D as weakly invasive on the basis of their relative activities in the chemoinvasion assay. In our previous work, the mRNA expression and enzyme activity of CD73 in such two breast cancer cell lines that differ in invasive and metastatic potential were analyzed by RT-PCR and HPLC. The results indicated the expression of CD73 was positively correlated with the migratory ability of breast cancer cells (Zhou and Zhou 2006). T-47D cells were weakly metastatic (Zajchowski et al. 2001) and had a nearly undetectable expression of CD73, while MB-MDA-231 cell was opposite. The ER(-) breast cancer cell line MDA-MB-231 with higher expression of CD73 expressed A2B adenosine receptor (Panjehpour et al. 2005). The fact that MB-MDA-231 cells derived from advanced metastatic breast cancer cells have significantly higher expression of CD73 compared to weak invasive breast cancer cell T-47D indicated that CD73 is a protein potentially involved in breast cancer metastasis. This study therefore focused on the potential implication of CD73 for breast tumor metastasis. The results showed that the ability of invasion, migration and adhesion of T-47D cells over-expressed with CD73 increased. Previous studies indicated that the CD73 might serve as a marker for migratory nerve cells (Schoen et al. 1988). In vitro, aortic endothelial cells (ECs) from CD73−/− mice display an up-regulation of mRNA and protein expression of vascular cell adhesion molecule-1 (VCAM-1) (Zernecke et al. 2006). Recent studies with human glioblastoma suggested that CD73 may serve as an adhesion molecule promoting tumor invasiveness (Fenoglio et al. 1997) and in the case of human lymphocytes it may mediate adhesion of lymphocytes to the endothelial layer (Airas et al. 1995). We investigated whether enzyme activity of CD73 promoted invasion, migration and adhesion of T-47D cells over-expressed with CD73. By pre-treating with APCP, the ability of invasion, migration and adhesion of CD73 gene transfected T-47D cells decreased. These results indicated that CD73 facilitated invasion, migration and adhesion of breast cancer cells due to its enzyme activity.
CD73 activities have been found in a variety of neoplastic cells. A highly active enzyme was detected in breast carcinoma, gastric cancer, pancreatic cancer, chronic myelogenous leukaemia, cutaneous T-cell lymphoma, and in Walker 256 carcinoma (Spychala 2000). CD73 could hydrolyze extracellular nucleotides into bioactive nucleoside intermediates (Zimmermann 1992). Being most abundant, adenosine, acting through specific receptors, is implicated in many physiological and pathophysiological processes, including regulation of tumorigenesis (Madi et al. 2003; Merighi et al. 2002; Woodhouse et al. 1998). The concentration of adenosine in the extracellular fluid of solid tumors was higher than normal tissues and tissues around tumor (Blay et al. 1997). A1 receptors mediate motility and chemotactic response to adenosine in melanoma cells. Some experiments revealed that adenosine regulates the CD73 expression and barrier function of vascular endothelial cells by paracrine pathway (Narravula et al. 2000). Extracellular adenosine has been shown to enhance vascular barrier function by activating endothelial A2B receptors (Lennon et al. 1998; Richard et al. 1998) and to interfere with leukocyte-endothelial adhesion via occupancy of A2A receptors on stimulated neutrophils (Cronstein 1994; Sitkovsky 2003). Furthermore, adenosine has been shown to stimulate canine retinal microvascular endothelial cell migration and tube formation (Lutty et al. 1998; Lutty and McLeod 2003). Therefore, it was not surprising that adenosine has been shown to be a crucial factor in determining the cancer cell progression pathway (Merighi et al. 2003).
Thus, we speculate that CD73 could promote invasion, migration and adhesion to ECM of cancer cells through it enzyme activity in generating adenosine. Breast cancer cell lines with high and low expression of CD73 were selected to prove this concept. The results demonstrated that adenosine promote invasion, motility and adhesion of breast cancer cells in vitro.
In order to further investigate the effect of CD73-generated adenosine on invasion, migration and adhesion of breast cancer cells, mammary carcinoma cells MDA-MB-231 which over-express CD73 were selected as a model of aggressive cancer cells. The ability of invasion and migration of MDA-MB-231 cells were significantly inhibited by APCP due to suppression of CD73 enzyme activity. Previous evidences indicated that CD73 purified from chicken gizzard was shown to specifically interact with laminin and fibronectin (Stochaj et al. 1989 ). Both Fibronectin and collagen are fundamental components of ECM. The effects of CD73 on the adhesion of breast cancer cells to fibronectin and collagen were observed, respectively. The results demostrated that adhesion of MDA-MB-231 cells to fibronectin and collagen was inhibited by suppressing CD73 enzyme activity.
In summary, our study found that CD73 promoted invasion, migration and adhesion of breast cancer cells through CD73 enzyme activity in generating adenosine. CD73 may have pro-metastasis activities and may serve as a diagnostic marker and therapeutic target of breast cancer.
We thank Dr. Linda Thompson for providing pBluescript SK(+) plasmid.


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Da, Da, Da. Da Dada Da. Dada dada dada da dadada. While that may sound like nonsense, if I told you it's my best attempt to spell out the Super Mario Bros. tune, it becomes immediately recognizable. If you were born after 1970, chances are you can hum the theme from the first board of that game (and probably the underground second board) like it's the most natural thing in the world. In fact, even novice gamers can probably hum the tunes from all the major Mario titles of the last twenty... READ ON
I guess with all of the election stuff going on here in the States, we here at the _floss completely overlooked that yesterday was Guy Fawkes Day. While it may not be a huge celebration (or even a celebration at all) in the U.S., it's quite a big deal in Great Britain. It's also observed in certain parts of Canada, South Africa, the Caribbean and New Zealand). So, in at attempt to make it up to the late Mr. Fawkes, I thought we'd honor him (I suppose "honor" him would be... READ ON
Physics professor Mark Newman has a thing for maps. He co-authored The Atlas of the Real World, a volume that features cartograms, maps in which a mathematical variable is substituted for land area. This causes traditional land-based maps to skew and distort in bizarre ways, hopefully communicating important information to the viewer -- information that goes beyond what you can see when just looking at land area.
Newman's cartograms of the 2008 election map have been making the rounds of the web, and... READ ON
The other day I spent five-and-a-half hours commuting and never made it to work. My journey was plagued by major subway shutdowns, massive bus delays and general bad luck. But two good things came out of this:
1) While waiting for a bus back to NJ, I witnessed a loud but civil argument over whether a woman had cheated on her man while he was in jail. His evidence: "There was 2 condoms gone from my Ziploc. I rest my case." (He kept repeating the second part.) They were waiting for... READ ON
I've been shocked by plenty of faulty lamps and even backed into an electric fence one time, so I know how un-fun having unwanted electricity coursing through your body can be. But what about much lower doses of exposure, over long periods of time? Electric fields are everywhere, but aside from the vague sense that positioning my face near a turned-on microwave is a bad idea, I never thought much about the health implications of everyday EMF. That is, until I found a handy little pamphlet enclosed with... READ ON
In an interview with the New York Times Magazine, Christopher Walken said, ''I'm a terrible analyzer of what will be good. Whatever I think the outcome is going to be, I'm always wrong.'' One look at his resume shows just how right he is.
Of the terrible movies in this quiz, can you identify which ones Walken was in?
Take the Quiz: Christopher... READ ON
In his victory speech on Tuesday night, Barack Obama promised his daughters Sasha and Malia that they'd get to bring a new puppy with them to the White House in January. It's a good thing Obama said "Yes, we can" to the girls' request; for all of his charm, ability, and oratorical flair, he could never be our nation's chief executive without a White House pet. Counting Obama, the country has had 44 Presidents, and only two of them—Chester A. Arthur and... READ ON
Australians are proud of their wildlife, which is not only the oddest in the world, but also the most dangerous. That's not to say that it's a dangerous country, because most animals do not go out of their way to attack people. You just don't want to take any chances by provoking the native species.... READ ON
With all the dozens and dozens of Word Wrap posts, I'm flabbergasted that we've never covered menu typos—something that seems like such an obvious topic for a site like this. Of course, the best menu typos can be found in Greece, where lamb is misspelled all over the country as lamp. If you've ever been, then you know it's like striking gold: you get the best food in the world, and have the most fun ordering it.
Closer to home, there's a Mexican joint near me... READ ON
There are two questions in today's Brady Brain Game, and they both have to do with the show's opening theme song. The "rule" is that you get to watch the video clip once before answering the two questions, so do that now. (And remember, a Brady never breaks a rule.) Here's the first-season version:
And now, your two questions:
1. Which is the only Brady child specifically referred to in the song's lyrics?
2. What's the sum total of all the numbers mentioned in the song's lyrics?
The Road to the Inauguration. Newsweek takes an in-depth look at the secrets of the 2008 presidential campaign in a seven-part series.
Do you know a manly man, a man you can look up to, who epitomizes the best in manliness? Then you're lucky! Nominate him now for the Man of the Year award from The Art of Manliness.
The average hand in a study of 51 college students had 150 different species of bacteria on the skin. If that doesn't make you want to go wash your hands right now, I don't know what... READ ON
Quick reminder: we're accepting pre-orders for our "D'ow!" financial meltdown t-shirt. It's the perfect gift for the person who lost everything (including his or her graphic t-shirt collection) but has a sense of humor about it.
In this case, you can pre-order by emailing email@example.com and saying that you want one. Also, tell us your size and shipping address. Once we know how many to print, we'll get back to you to complete the transaction. They're going to cost $17.97.
If economic crisis... READ ON


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HostMonster Web Hosting Help
Search results for "stats" - 25 results
To view your stats, scroll down to the Logs section of the cPanel Here are different programs that can be used to view your stats. We most recommend awstats When you select your stats program, you ma
WebStats and Features.
I would like to view the statistics for my website.
Knowledgebase Article 143,637 views tags: addon domain enable hits set setup statistics stats subdomain visitors web
How to enable the function to manually update AwStats
How can I import awstats from my previous host?
Learn how to check your email accounts from within Gmail. This tutorial will show you how to setup a POP account in GMail.
I get the following database connection error: Warning: mysql_connect() [function.mysql-connect]: Host 'host#.HostMonster.com' is blocked because of many connection errors; unblock with 'mysqlad
Knowledgebase Article 116,891 views tags: blocked connection host localhost mysql
What is SSH and how do I setup secure connections.
A list of common log files and their locations.
Knowledgebase Article 4,623 views tags: dedi dedicated directory file folder list locations logs vps
I cannot connect to my database locally or remotely, do I have the correct server, username and password?
This article will explain how to clear the outbound mail queue on a dedicated or VPS server.
Allowing access to Awstats without requiring a cPanel login
Knowledgebase Article 224,345 views tags: awstats
How do I publish my website using Microsoft Publisher 2007
Getting Started Wizard After you have installed phpBB, please use the administrative link provided in SimpleScripts to access the login area. Enter the Admin Username you selected when installing php
How do I use the free shared SSL Certificate?
Knowledgebase Article 300,336 views tags: certificate path shared ssl
How to setup email on the Windows Phone
This article will explain how to log in to your Account Control Panel
With support for FrontPage extensions now gone you may want to find alternatives to email forms and the counters that are native to front page. This article explains some ways to replace the forms and hit counters.
Knowledgebase Article 9,293 views tags: alternatives counters forms frontpage mail
This article will outline which PHP PEAR Packages are installed and how you can install additional PHP PEAR Packages.
How do I upload my web site?
How do I use .html files to run SSI (Server Side Includes)?
This article will explain how to run php and other file types using Cron jobs.
Here is a good list of basic SSH commands.
How to manage CloudFlare on your HostMonster account.
Knowledgebase Article 155,122 views tags: cloudflare dns
What is the .htaccess file and where can I find more information?


text:
South Boston News & Record
and Mecklenburg Sun
More charges contemplated in fatal Vernon Hill crash
08/26/16 - 1:07 pm
As the investigation continues into the Aug. 20 traffic crash that claimed the life of 9-year-old Julian Adam Suttle, Virginia State Police and the Halifax County Commonwealth's Attorney are currently…
Medical missionary
08/25/16 - 10:53 am
JMU student receives hands-on lessons in Third World health
Halifax County supervisors advised to borrow $16 million for courthouse
08/25/16 - 10:50 am
Ricketts - Hold off on full amount till needed
Comets take positive step forward
08/29/16 - 7:55 am
Pound Patrick County 42-12 in season opener
- More A&E
Charlotte County woman dies in crash outside Clarksville
SoVaNow.com / July 03, 2013A Charlotte County woman died in a two-car crash outside of Clarksville Thursday night.
Virginia State Police responded to Highway 15 south near Mt. Ararat Rd. at 11:00 p.m. Thursday after receiving reports of a head-on collision. According to Trooper J.A. Williamson, a 2004 Grand Marquis driven by 71-year-old Frederick W. Gaston of Clarksville crossed the center line and collided with a 1993 Geo Prizm driven by 24-year-old Lindsey G. Claytor of Charlotte Courthouse. The impact of the crash forced the Prizm off the right side of the road where it struck the guardrail.
Claytor died at the scene. A passenger in her vehicle, 28-year-old Anthony Irving, was transported by emergency personnel to an area hospital, as was Gaston.
Williams said both drivers were wearing their seatbelts.
No charges have been filed at this time. The crash remains under investigation.
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There is a class 5 typhoon, Neoguri, headed to our little island and it is suppose to hit us within the next 24 hours. Normally, I'd be happy for the forced down day to relax inside my cement-made-for-typhoons-home, while nature has a temper tantrum outside….
I'm on the little speck named Kadena, right along that pink path.
In fact, I should be particularly excited because this is the first typhoon I get to have with my handsome husband, instead of just me and my cat. He was deployed during all the typhoons last year.
Typhoons around here, are like a snow day. Every structure on island is made to withstand multiple typhoons, every year. As long as you prepared; tied down things that could fly away, stocked up on food and water, and prepared for the possibility of the power going out….. there really isn't anything to be nervous about.
So, why the change of heart? Because today is my due date. Can you imagine trying to drive to the hospital in the middle of the biggest typhoon this island has seen in 15 years? Or delivering your first baby at home by yourselves?
Neither can the hospital staff. When the storm gets close, the hospital will start excepting all pregos within 3 weeks of their due dates and their plus ones. We will all get set up in a large room with cots to sleep on.
A cot. I'm not excited about that. Also the image of me and every other pregnant woman waddling in there with our millions of pillows tucked under our arms… it is just comical.
On top of this situation… have you heard about the change in barometric pressure during a storm causing women to go into labor? After a short google search (what I did to see how much weight this theory has) it's easy to see that, while there is not a clear conclusion, it is most certainly loosely connected. The change in pressure outside can cause the membranes inside to rupture and to speed up the labor process. All studies I read were unanimous on the fact that, during storms, the number of pregos giving birth increases.
So now I'm thinking there is a very likely chance that I will have a typhoon induced labor. The first part of my birth plan is out the window in that situation.
I can't labor at all in my own home. There goes my plan for those yummy popsicles in my freezer. And that glistening, just cleaned bathtub.
My doula can't come, unless I go into labor before they close down the roads. If you don't know what a doula is, just know that she is a wonderful lady that helps the family before, during, and after labor. I really want her to be there.
I have to leave my kitty at home by herself. I know, I know…. I'm a crazy cat lady to worry about her. But I can't help it. I don't want my furry friend to go through the stress of a storm without her humans.
And I think my tiny Japanese car might fly away, like a plastic bag, in the hospital parking lot. Even though we did our best to make it extra heavy by filling the gas tank and leaving the sandbags we didn't use in the back of the car. Not like either will help if it catches a gust of wind.
This is why I think that my car might be tossed around: Category 1 typhoon, Jelawat, that hit Okinawa in 2012:
I may have told myself that everything is going to be okay enough times now, that I can calmly head to the hospital tonight feeling like that it is so. I will still feel guilty for leaving my kitty and I'll be shocked when we emerge later to find that our car is still in working condition in the parking lot, but we can work with this.
And who knows? I might get to hold my baby girl a few days earlier than I would have if this typhoon didn't decided to blow through?
If she is born during the storm she'll have a great nickname, Neoguri. Which means raccoon dog in Korean. I think I will call her that when I'm angry.
Come back soon to find out how things went…. I'm curious too.


text:
Porta Bella Dock No 1 (Infilled)
The entrance is crossed by Towpath Bridge No 5Also known as: Kensal Green Gasworks Docks
Harrow Road, London, Royal Borough of Kensington and Chelsea, Greater London, England, United Kingdom
Porta Bella Dock No 1 (Infilled) is a minor waterways place on the Grand Union Canal (Paddington Branch) between Bull's Bridge Junction (Junction with Grand Union Paddington Branch. Opposite is a dry dock.) (11 miles and 1¾ furlongs to the west) and Little Venice (Junction of Grand Union Regents Canal and Paddington Branch) (1 mile and 6½ furlongs to the east).
The nearest place in the direction of Bull's Bridge Junction is Kensal Green Visitor Moorings (14 day moorings (14 days in one year - £25 per day for overstaying)); 1½ furlongs away.The nearest place in the direction of Little Venice is Kensal Green Stop & Shop Moorings (4 hours moorings); ¼ furlongs away.
Places of interest within 1 mile and to which this is the nearest place: Mainline railway stations (3517 away); B and Q DIY shops (3475 away); Barclays Bank cashpoints (ATM machines) (3273 away); Nationwide Building Society cashpoints (ATM machines) (3231 away); Natwest Bank cashpoints (ATM machines) (3300 away); Santander Bank cashpoints (ATM machines) (3260 away)
|Railway Bridge No 7D
|6¼ furlongs
|Mary Seacole Gardens
|6 furlongs
|Mitre Bridge No 6
|6 furlongs
|Mitre Wharf Moorings
|5½ furlongs
|Kensal Green Visitor Moorings
|1½ furlongs
|Porta Bella Dock No 1 (Infilled)
|Kensal Green Stop & Shop Moorings
|¼ furlongs
|Porta Bella Dock No 2
|¾ furlongs
|Kensal Green Bridge No 4
|1 furlong
|Kensal Green Arm
|1¼ furlongs
|Ladbroke Grove Footbridge No 4AB
|1¾ furlongs
User contributed information about Porta Bella Dock No 1 (Infilled)
Sainsbury's, Ladbroke Grove, has visitor moorings adjacent, to the west of the dock armThis information may need editing and reformatting for appearance and clarity
1 vote cast
Mooring here is "good" - a nice place to moor
Users' comments on mooring here
No comments yet!Help
Click on a photograph to visit it on Geograph.
These pictures are licensed under a Creative Commons license and copyright the photographer shown above
These pictures are licensed under a Creative Commons license and copyright the photographer shown above
Information retrieved Friday 19 August 2016 at 11:39
There are no links to external websites from here.
Why not log in and add some from the "add" submenu?
Why not log in and add some from the "add" submenu?
There is no page on Wikipedia called "Porta Bella Dock No 1"
Wikipedia pages that might relate to Porta Bella Dock No 1[2010–11 Club Nacional de Football season] [Mayagüez, Puerto Rico] [Silvio Berlusconi] [List of Latin phrases (full)]
Information retrieved Monday 22 August 2016 at 15:02
- Ten Health & Fitness (Physical Fitness)
397 to the south.
Spasbeautypersonal care
- Max Borges Hair Salon (Beauty Salon, Hair Salon, Hair & Beauty Supply)
172 to the northeast.
Local business
- Kensal Green Moorings (Boating)
63 to the southeast.
- Kensal Green Cemetery (Funeral Service)
430 to the west.
- Queens Park, Northwest London (Park)
947 to the northeast.
- Paddington Central London (Train Station)
292 to the southwest.
- Sainsbury's (ShoppingRetail)
153 to the southeast.
- Vicki's (Deli)
321 to the northeast.
- Kensal Rise railway station (Train Station)
804 to the north.
- William IV (Pub, Performance Venue, Bed and Breakfast)
198 to the north.
- Nando's (Chicken Restaurant)
470 to the north.
- Maida Vale London UK (Region, Community & Government)
1375 to the east.
- Sainsbury's Ladbroke Grove (Grocery Store)
174 to the southeast.
- The Paradise (Gastropub)
241 to the northeast.
- Innocent Fruit Towers (Workplace & Office)
263 to the east.
- The Sleep Room (ShoppingRetail)
452 to the south.
- Fierce Yoga West (Yoga & Pilates)
857 to the northeast.
- London Euston Train Station (Train Station)
767 to the northeast.
- Portobello Market At Nottinghill (Market, Place to EatDrink)
1458 to the southeast.
- Nothing Hill (Portobello Road) (Neighborhood, Market, Shopping District)
1446 to the east.
- Willesden Junction London Overground and Underground Station (Train Station)
1402 to the northwest.
- FRAME Dance, Yoga & Fitness Studios (Sports & Recreation)
819 to the northeast.
- Regents Canal (Lunch Place)
144 to the southeast.
- Amor (Organization)
542 to the northeast.
- Emslie Horniman Pleasance Park! (Outdoors)
661 to the east.
- The Bartoli Gallery (Wine Bar, Night Club, Pizza Place)
438 to the south.
- MODE (Night Club)
1172 to the southeast.
- Parlour (Gastropub)
221 to the northeast.
- The Whippet Inn (British Restaurant, Beer Garden)
559 to the north.
Museumart gallery
- Serena Morton Gallery (Art Gallery, Museum)
489 to the southeast.
Concert venue
- Loft Studios-Events (Event Venue, Movie & Television Studio)
1229 to the west.
Professional service
- JF Car Service (Travel & Transportation)
1005 to the north.
- La Pia (Pizza Place, Italian Restaurant, Coffee Shop)
432 to the southeast.
- Dock Kitchen (Modern European Restaurant)
276 to the east.
- Nando's Kensal Rise (Portuguese Restaurant, Chicken Restaurant, Fast Food Restaurant)
414 to the north.
- Rullos London (Italian Restaurant, Fast Food Restaurant)
554 to the north.
- Portobello Juice café (Smoothie & Juice Bar, Breakfast & Brunch Restaurant, Salad Bar)
1154 to the southeast.
- Sacro Cuore Pizzeria (Italian Restaurant)
555 to the north.


text:
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text:
Below: An overgrown view of Dewey's House and Connect Dewey when it's not covered in children
After you tour through Dewey's house, you'll flow with the crowd into his Meet 'n' Greet Conservatory in the back yard. There you can observe Dewey who has just finished animating a scene from his latest film, "The Banned Concert". Be sure to ask him a silly question!
Don't click Princess Mode
© copyright 2011–2016
A Pants Aflame production


text:
In the Shade: Shady Shots
Mlee from Hearts of Animals tastefully sets up her gear.
Chris Cascio (Wicked Poseur, Artstorm) stands by as Mlee sets up.
ADR and Erik Bogle (Bring Back the Guns, Fatal Flying Guilloteens) discuss NPR over iPhone.
Andrew Lee of The Wild Moccasins.
-Dusti Rhodes
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text:
The state will spend up to $500,000 to furnish the new Anchorage legislative information office.
The Legislative Council, which handles office policy for state lawmakers, awarded the contract to Think Office, LLC, at a Wednesday meeting. The furniture will come from the modern design company Knoll, and the look of the new building will be up-market Ikea. To give an idea of what they were looking for, the Legislative Council bidding notice listed rolling chairs that retail for $1,500 and $300 coat racks made of colorful metal rods.
The Think Office bid conforms to a decorating plan created by Pfeffer Development, which is renovating the Anchorage LIO and was awarded a $100,000 contract specifically for furniture consulting. The objective was to standardize design using modular components in an effort to reduce the need for new furniture when freshman legislators come into office and as political organization changes.
When the Legislative Council first took up the question of furniture procurement in early June, the initial price tag was $866,000. That triggered sticker shock, and a furniture subcommittee led by Chugiak Republican Bill Stoltze was created to reexamine the cost. The subcommittee concluded that the Anchorage LIO should be outfitted with surplus furniture from state agencies, with a cap of $100,000 for new furniture.
But that recommendation was rejected. Legislative aide Juli Lucky explained to the council that number was unrealistic.
"I will admit that the office furniture was a lot more expensive than I had anticipated," said Lucky. "But I do feel that we did come up with a good compromise position once we did look at the comparable offices that we have outfitted in other places. We did winnow down that cost as much as we felt we could."
About half of the $500,000 award will be spent furnishing legislators' individual offices, at a cost of $8,000 per lawmaker.
Rep. Mike Hawker, an Anchorage Republican who chairs the Legislative Council, said that the expense of furnishing the Anchorage LIO compared favorably to other projects.
"A couple of years ago when the Eagle River LIO was refurnished, those offices ran $20,000 a copy," said Hawker. "So, I think we're getting quite a value."
The other half of the money will go to furnishing the building's public areas, like hearing rooms.
"They can be used for all of the summer sort of meetings like we're doing right here, and they can be used to accommodate special legislative sessions with a chamber for the House and the Senate, as well as a chamber that would accommodate 60 legislators as needed," said Hawker.
While cost was a sticking point for some, Sen. Dennis Egan, a Juneau Democrat who caucuses with the Republican majority, took issue with the argument that the LIO needed to be fully furnished so that special sessions could be held in Anchorage.
"I really am upset that you're talking about special sessions being held in the Anchorage LIO," said Egan. "Special sessions should be held in the Capitol."
Hawker stressed that he did not mean to trigger a debate over the location of the Capitol.
"This is not a disguised Capitol move," said Hawker.
Egan ultimately voted for the contract.
The contract was awarded on a 10-2 vote, with Stoltze and Anchorage Democrat Max Gruenberg opposing the contract.
The Anchorage LIO project has previously faced criticism for its cost. The lease to the office is expected to run $80 million over the next 20 years, and Hawker's Democratic challenger Sam Combs described the furniture contract as an example of project "mismanagement."
Recent headlines
School enrollment numbers in Juneau buck declining trendSo far, the Juneau School District has enrolled about 230 more students than it expected. If the higher enrollment remains true in October, the district could get enough additional state funding to cover a near $200,000 deficit.
Assembly to hear Southeast nonprofit's appeal on denied tax exemptionsJuneau-based nonprofit, Southeast Alaska Land Trust, was denied its property tax exemption earlier this year. Now the Assembly will take another look.
NASA keeps watch of shrinking Arctic ice"A lot of ice experts, including myself, thought we were headed for a record year minimum," said Hajo Eicken, a professor at the International Arctic Research Center at the University of Alaska Fairbanks.


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I have a MA Teaching English to Speakers of Other Languages (TESOL), Madonna University, and a BA International Studies Business and French, University of Michigan-Dearborn. I am a university instructor with five years ESL tutoring experience. I am dedicated to instructing those who are interested...
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Columnist/Journalist/ Tech Writer, Hindustan Times, Delhi. Music and movie lover. Alleged poet. Views are personal. RTs not endorsements
RT @madversity: Via WhatsApp Sindhu, Sakshi & Dipa 🏅 make their debut straight on Pg.1. Shobha De 💃 after so many years still on Pg.3. 😂😆😝😃
@madversity @Svara so single parents can adopt a child in India but cant become biological parent of own genes ? What logic is tht?
@tshamsi88 Success for 2Day& 2morrow means playing 2 different Games simultaneously? @DrAshwani_Kumar @ShekharGupta @NitishKumar @madversity
RT @madversity: Via WhatsApp Sindhu, Sakshi & Dipa 🏅 make their debut straight on Pg.1. Shobha De 💃 after so many years still on Pg.3. 😂😆😝😃
@madversity somehow thr is tacit surrender that ALL wearing burka do by thr choice or culturalsymbol;exact same argument once used for sati!
RT @veejaysai: Hellooowwwww! Diplomats in Delhi obviously have very exciting lives.... @madversity @sanjayuvacha @MadrasMobile https://t.co/Atvh0iyXoC
@madversity why outrage against banning burka(penalty-$30)always more than against those enforcing it(penalty-death)?Surrender to religion?
RT @madversity: "France has "misunderstood" burkini, Australian designer says" - https://t.co/hOHIIa61hl
RT @madversity: Casteist oppression, religious bigotry and dynasticism are all forms of a parochial outlook.
@madversity so much importance to achievements than doing something out of pleasure/happiness or interest. Results for China, Pleasure4USA
RT @madversity: India will continue to be mocked. Hype for medals only shows misplaced priorities in a nation beset with poverty. pollution & parochialism
Gul fenke auron par, samar bhi/ E abr-e-karam, e-behr-e-sakha kuch to idhar bhi || ठाकुर जी के पंखे हो गए हैं हम तो इसके बाद से @madversity
@madversity @PMOIndia @HRDMinistry shame on us 7th grade students raise points which we should raise
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MTV VMAs: Beyonce wins the VMAs, Rihanna and Drake melt our hearts, Britney Spears fizzles
Los Angeles Times
It's fair to say Beyonce stole the MTV VMAs with an extended performance inspired by "Lemonade." It was also a special evening for Rihanna, who performed throughout the night before Drake presented the Vanguard award to the woman he's "been in love ...
- - -
Australian aid worker Kerry Jane Wilson released unharmed in Afghanistan after four-month ordeal
The Sydney Morning Herald
Australian aid worker Kerry Jane Wilson has been released unharmed after four months being held hostage in Afghanistan. Dr Wilson was snatched at gunpoint in late April by gun-toting men posing as Afghan intelligence officers from the office of her ...
Mars simulation crew 'return to Earth' after 365 days in isolation
(CNN) A crew of intrepid astronauts have emerged after a year on Mars... kind of. Six scientists spent 365 days in a geodesic dome set in a Mars-like environment 8,200 feet (2,500 meters) above sea level. The simulated habitat was located on the slopes ...
- - -
Colin Kaepernick: I'll continue to sit during National Anthem
SANTA CLARA, Calif. -- The word "cancer" was used by some 49ers players Sunday following the uproar over quarterback Colin Kaepernick's decision Friday to not stand for the National Anthem for a third time before a preseason game. Kaepernick's ...
- - -


text: AdChoices Terms of Use Privacy Policy Hide VIDEO Sports and Fitness Become An Optimist Video Become An Optimist Video The 3 Steps To Becoming An Optimist AskMen Editors August 2, 2012 Share Tweet 0 Shares It's easy to get drowned in negative thoughts, so editor Elysha Krupp breaks down a three step process to help you have a more positive outlook on life. Stress Positivity Optimism Comments Share your opinion Your name More Like This Health & Sports News Trending News: Want To Feel Less Stressed? Try Making This Small Change To Your Day Read More Career How To Stay Calm Under Pressure: 3 Simple Tips Read More Basics Don't Become The Target Of A Common Holiday Scam - Read This Read More Thrive Here's The Simple Secret To Making Every Single Workout A Success Read More Thrive How Orangetheory Fitness Is Changing Today's Gym Landscape Read More Thrive It's Seriously Time You Added Lateral Lunges To Your Workout - Here's Why Read More Around the Web Special Features Sponsored Content How To Dress Like The World's Most Stylish Men Read More Dating The Ultimate Guide To Hooking Up Online Read More Groming Guide 15 Best Beard Trimmers On The Market Read More Fitness Gear Finding The Right Gym Bag Read More Grooming Guide The Best Razors Of 2016 Read More Guy Gear The Best Watches For Men Under $500 Read More


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Lyrics Depot is your source of lyrics to SO YOU WANT TO BE A ROCK N' ROLL STAR by Tom Petty. Please check back for more Tom Petty lyrics.
SO YOU WANT TO BE A ROCK N' ROLL STAR Lyrics
Artist: Tom Petty
Album: Pack Up The Plantation
So you want to be a rock 'n' roll star?
Well listen now, hear what I say
Just get an electric guitar
Take some time
And learn how to play
And when your hair swung right,
And your pants get tight
It's gonna be alright
Well then it's time to go downtown
To the agent man won't let you down
Sell your soul to some company
Who are waiting there to sell plastic ware
And in a week or two if you make the charts
The girls will tear you apart
It's the price you pay for your riches and fame,
Was it all a strange game?
You're a little insane
The money that came, and the public acclaim,
Don't forget what you are,
You're a rock 'n' roll star
Tom Petty Lyrics
Tom Petty Pack Up The Plantation Lyrics
More Tom Petty Music Lyrics:
Tom Petty - Accused Of Love Lyrics
Tom Petty - MAKE IT BETTER (FORGET ABOUT ME) Lyrics
Tom Petty - Money Becomes King Lyrics
Tom Petty - NEEDLES AND PINS Lyrics
Tom Petty - Stop Draggin' My Heart Around Lyrics
Tom Petty - THE SAME OLD YOU Lyrics
Tom Petty - THE WAITING Lyrics
Tom Petty - Walls (Circus) Lyrics


text:
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The Oakland Raiders are the dark lords of the National Football League. Known for their SIlver and Black attire and a fan base who looks as though they came to the stadium directly from the gates of hell. So it is only fitting that when someone decided to create Star Wars logos for every NFL team, the Raiders received one of the most epic characters to grace the trilogy (Sorry, new movies suck and I like to pretend they never happened).
So without further ado, I present to you, the Oakland Vaders


text: Me and my boyfriend went there for a dinner, we wanted to try some traditional Sardinian food and we couldn't choose a better place. The agriturismo is quite far from the main road, but there are signs so you can't get lost. We were the only one having dinner there that evening. We were sited and the owner started bringing... 更多


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Meg Seidel / Collections / Groups
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I am hoping after effects users will use this space as a center for advice, tutorials, collaboration and exploration in the world of using Adobe After Effects. Adobe After Effects is one of the leading…
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Bike + camera - what have you got? A group for bike-mounted cameras of all kinds. Let's experiment.
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WELCOME TO THE VIMEO GROUP FOR DEDICATED USERS AND FANS OF CANON REBEL T3I / 600D / KISS X5. Photographers looking for an easy-to-use camera that will help them create their next masterpiece need…
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For those of you who aren't another "face" in the crowd of the millions of filmmakers out there. The group of the few. The group that exceeds the limitations that our society knows…
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The best Experimental Video Art Vimeo has to offer. El mejor Video Arte Experimental que vimeo puede ofrecer.
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Interesting and artistic projects taking place in and around Austin, Texas. The weirder the better!
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Where stills move.
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This is the timeLapse group for all your timelapse videos. Anyone can add videos, if possible please add timelapse info. To learn more about TimeLapse this is a great site to start: http://www.prestonkanak.com/extensive-raw-time-lapse-tutorial/ the…
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text:
Explore Floppy Art, Inch Floppy, and more!
via tumblr | #mixed_media #collage Reminds me of Melinda Gibson's photo collages and Rene Magritte's surreal paintings.
Learn more at beauxness.tumblr.com
from Saatchi Art
Artist: Loui Jover; Household 2015 Painting "perhaps "
from Saatchi Art
Artist: yossi kotler; Digital 2014 New Media "wondering"
Surreal pieces of Art by Antonio Mora, a Spain-based artist who spent several years working as an art director in the industry. Mora — now a full-time artist — describes himself as someone who creates portraits from images found on the Web, blogs, and magazines. He then merges the images, creating pieces of art with great force and expression that surely won't leave you indifferent
Learn more at clipzine.me
from Saatchi Art
Artist: Loui Jover; Ink 2014 Drawing "one perfect day"
NEW !! - mylovt - Antonio Mora - takes found photos and creates collages.
Learn more at lovely-market.fr
Powerful Paintings by Andrew Salgado Andrew Salgado is a Canadian artist based in London. His powerful, colourful portraits are focused on themes such as identity, sexuality and convalescence.
Learn more at theinspirationgrid.com


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Nick Oliver September 14, 2012 at 9:54pm
Keep up the good work, Ben. I know I'm not alone when I say thanks and good job. I'm supposed to take the fam on a mini vacation Wed n Thur, but if not I'm down to help.
Nick Oliver September 14, 2012 at 5:25pm
Keep up the good work, Ben. I know I'm not alone when I say thanks and good job. I'm supposed to take the fam on a mini vacation Wed n Thur, but if not I'm down to help.
Ben Calhoun September 14, 2012 at 3:23pm
Course is freshly mowed- let me know if you spot any demolished baskets. Speaking of hole C, if all goes well it should be replaced by next weekend. I have the replacement basket, working on securing it together today. Bill G is bringing a spare collar on Monday, and I should be able to dig the hole and pour cement Wednesday (could use some help need a volunteer), the collar will be moved approx. 30 ft. from where it is now. After the basket gets replaced, I plan to try to fix the bent one so we can have multiple placements on that hole.
Andrew Boyle September 13, 2012 at 3:10pm
lost my orange blizzard champion boss on hole 12. into the pond on the left. has name and number on it. will be reimbursed.
Nick Oliver September 12, 2012 at 5:40pm
I'm part of a group trying to put a permanent course on Belle Isle. Theres a clean up for the upcoming Oct 27th +28th tournament happening THIS SATURDAY! If interested contact me, or if your on Facebook http://www.discgolfscene.com/tournaments/Battle_at_the_Belle_2012. Lets work together and create a Detroit landmark for the disc golf world!
Jason Cassidy September 3, 2012 at 6:35pm
I would be there!
Ben Calhoun September 3, 2012 at 3:27pm
Any interest in a Jay and Des Reading clinic again, this Wednesday?
Ben Calhoun August 31, 2012 at 5:34pm
BIG Willow on #5 down
Shaun Adkins August 30, 2012 at 2:53pm
I think kt lost one.
Ben Calhoun August 30, 2012 at 2:49pm
Anyone lose any discs (out in the open, not the water) this week? We've had 3 turned in found out in the grass..1 was really nice, found Tuesday night, it probably belongs to someone who played the league. No names on any.
Douglas McIntosh August 27, 2012 at 8:46pm
Tomorrow Tuesday Aug 28th, Huron Valley PDGA Sanctioned Singles is heading back to Lakeshore to play 18 holes from the long tees. $1 to get your round rated and there are other side bets like ace pool and CTP. There is no extra fee if your not current with the PDGA. Tee off @ 5:30pm
Following weeks
Sept 4th - Willow
Sept 11th - Orginal
Ben Calhoun August 24, 2012 at 4:10pm
Anybody have a portable welder? A weld holding the D-hook for a chain link broke off W's basket sometime this week, somehow.
Nick Pacific August 19, 2012 at 3:31pm
Lost a black Pro D Challenger in the pond. Unmarked.
Shane Brayton August 19, 2012 at 4:05am
Lost a orange JK Aviar in the middle of the pond, willing to give a reward if it's returned.
Felicia Calhoun August 18, 2012 at 2:19am
But have you ever seen one go into a pond about nine feet deep to get discs.
Felicia Calhoun August 17, 2012 at 5:09pm
Lol not really Mike but whatever.
Mike "Papa Par" Even August 17, 2012 at 5:02pm
Don't you mean Dirty Bum?
Felicia Calhoun August 17, 2012 at 4:45pm
Ryan i was talking about another disc bandit thats on the loose.
Felicia Calhoun August 17, 2012 at 12:13am
Well there is more then one DB out there at lakeshore! Just a heads up!
Angel Gilbert August 16, 2012 at 10:46pm
i would use the like button for gabes two statements and joe p. those were great. cant say this is a fav course but i would play it any day over cass for sure everyone has been saying horrible things about it lately.


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The Noisy Thunder Planet Railroad attraction, mechanically considered to be the only overcrowded attraction at Disneyland
Board the Noisy Thunder Planet Railroad in the remains of Double Rainbow Ridge, then hold on to your head as you go on the craziest tour in the desert! While racing through Noisy Thunder Planet Railroad, you'll see dinosaur barbecue ribs, whiplashed cactus, and even a goat decaying dynamite!
Avoid Zombie Mode
© copyright 2011–2016
A Pants Aflame production


text:
Discover a selection of top forums stuff electronics on the internet. Come to share your passion on forums stuff electronics and meet other fans of stuff electronics. Then, why don't you create your own forum of stuff electronics ?
A place for gamers to talk about games and stuff.
begone, forum, free, browser, shooter, brotherhood, unity, game, community, online, based, multiplayer, guerra, first, person, universal, gaming
A cricket forum. Flaming Bails. cricket forum stuff nonsense England Australia India Pakistan South Africa West Indies Sri Lanka Bangladesh Zimbabwe New Zealand IPL ashes irreverent message board
cricket, #stuff, nonsense, england, australia, india, pakistan, south, africa, west, indies, lanka, bangladesh, zimbabwe, zealand, ashes, irreverent
Our members offer cheap giftcards and discounted brand electronics at amazing prices! Automatic escrow system active to protect both seller and buyer with zero fees! Join as a seller and help out the community with amazing offers or become a buyer
discount, giftcards, #electronics, cheap, discounted, brand, amazing, prices!, join, seller, buyer, automatic, escrow
Here is our Forum Feel Free to Browse Around and Look at the Diff Selections we have They Range From RP to Battle to Gaming stuff etc so enjoy.
touhou, fans, feel, free, browse, diff, selections, range, battle, gaming, #stuff, enjoy
Free forum : a place to store my stuff
Old Stuff
My Interests


text:
Many of the New Year's resolutions people make involve self-promises to behave in a more healthy way. I thought of this while getting ready to throw out a Chinese-language U.S.-published weekly magazine that a Chinese friend had used to cushion a present being sent me in a mail package. I couldn't read the Chinese, of course, but a full-page ad on the back page caught my eye. It was an ad for various vitamins, and, amidst all the Chinese, there was one piece of text in English clearly visible on each bottle: "Made in U.S.A." (Several of the bottles displayed American flags on the containers.)
This caught my eye because I know that health-conscious Chinese tourists coming to the U.S. to visit often buy vitamins to take home with their Louis Vuitton handbags and Jaeger-LeCoultre watches. They don't trust Chinese-made vitamins -- manufactured in an environment where safety regulation, to put it gently, is a work in progress -- worrying that they are adulterated at best and dangerous at worst. So the Made in America cachet signifies safety, backed up by U.S. government Food and Drug Administration inspections. Hence the ad I saw.
However, last fall, during a visit to a specialist about a health issue, I asked the doctor whether I should take a certain vitamin to help. He looked at me and said that not only was there no evidence that this vitamin helped (a problem that is occurring increasingly in studies, unfortunately), but that he himself avoided all vitamins because most of the ones we buy in the U.S. are -- get ready for this! -- made in China and probably harmful. In theory, Chinese-made vitamins had to meet FDA manufacturing standards, but he was not confident that the FDA has the inspection resources to check on compliance with the regs.
I proceeded to check the bottles of the vitamins I had at home. To my surprise, I discovered that none of them listed a country of origin. This was surprising, since I assumed that the law required that all products sold in the U.S. indicate where they are made. It turns out, however, that this rule does not apply to over-the-counter medication, and that a large proportion of vitamins sold in the U.S. (and aspirin as well) are actually manufactured in China. I would love to know the history of this exception to the law, particularly inappropriate since there may be a health issue at stake. (I will confess -- at the risk of generating hate posts to this blog -- that I am by no means normally a rabid made-in-America only kind of guy.)
Does any blog reader know any more about this? It strikes me as particularly ironic that many Chinese tourists may be buying vitamins in the U.S. because they trust our regulatory system more, only to be unwittingly purchasing vitamins from their own country.
BTW, a happy 2012 to all blog readers!
Posted on Jan 03, 2012 at 12:09 PM
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In the latest issue: grid security, agile development, BYOD and more!
Get the full issue
Steve Kelman contends that a recent report on agencies' prize challenges tells only half the success story.
OMB's Data Center Optimization Initiative gives agencies new metrics and goals for fiscal 2018.
NSA and the CIA have revamped themselves in the past year. FCW asked officials how well Fort Meade and Langley are collaborating in cyberspace in pursuit of their separate missions.
The latest version of the Defense Department-wide Enterprise Service Management Framework places more emphasis on managing IT risk.
Despite GSA's efforts to accelerate the FedRAMP approval process, the lack of agency reciprocity puts the program's central goals at risk.
Got some early-career colleagues who are doing great things in federal IT? Nominate them for FCW's 2016 Rising Star awards.
The agency software inventories required under the draft category management policy will be treasure maps that lead to efficient, effective, streamlined buying and unprecedented savings.
As experts warned of the "dire" threats posed by outdated federal technology, lawmakers grilled top feds, debated workforce issues and inched closer to backing a $3.1 billion fix-it fund.
The agency is making strides in developing and deploying border technologies but still has some weaknesses.
Nature Made is a company owned by the Primivite owned by the Japanese. Not American.
I have unimpeachable information that store brand vitamins made at large warehouse stores are made from raw materials sourced in Kazakhstan, Nigeria, and
East Tior.
Also, I know with 100% certainty that Nature's Bounty is manufactured using imported Vitamin raw materials from China.
The OTC and supplement mfg would also not want to have an FDA study of their product find and report that the supplement does not perform as advertised... Tough to sell things that really don't work...
Nature Made and Nature's Bounty are both made in the US
Show All Comments
8609 Westwood Center Drive, Suite 500Vienna, VA 22182-2215 703-876-5100
© 1996-2013 1105 Media, Inc. All Rights Reserved. This copy is for your personal, non-commercial use only.To order presentation-ready copies for distribution to colleagues, clients or customers, visit: www.1105Reprints.com


text:
Comfy Apartment in the Center
Størrelse50 m²
Etasje1(med heis)
- Familie / Barne vennlig
- Internett/WiFi
- Husdyr er tillatt
- Heis i bygningen
- Låsbare rom
- Håndklær
|Thokoly, 1076 Budapest, Ungarn
|Eiendoms ID
|Innen noen få timer
|Kalender oppdatert
|I dag
The apartment offers you modest, clean comfort with excellent infrastructure (Metro in 2 corners distance) nice, spacious rooms, TV, kitchenette, WIFI free of charge, running hot water all day and night. It is suitable for up to 4 people and offers provides them 2 comfy bedrooms with 2 single beds in each bedroom and also a complete bathroom.
Discover the atmosphere of the real city in a house built on the turn of the 19th-20th century, take a walk to the wonderful City Park, enjoy Szechenyi Spa's world famous services, visit Heroes Square, try the typical Hungarian kitchen on the way back home. Take the road to the left and walk downtown to Vaci Street, and simply take the night bus on your return and hop off just by your house.
You have your key on your own, move freely whenever you feel like
A damage deposit of 50 EUR may be asked on arrival, which is fully refundable unless damage is done.
|Ukentlig rate for opptil 2 gjester
|Månedlig rate for opptil 2 gjester
|Pris for >2 gjester
|107 NOK pr. gjest/natt ekstra
|Innsjekking etter
|Etter avtale
|Check-in før
|Etter avtale
|Utsjekking før
|Etter avtale
Clean and comfortable apartment with high ceiling and retro furniture. Spacious and airy. Central location. Well priced. Caring, helpful host. Recommended.
Lovely apartment close to Metro and overland rail links.
It's also close to the wonderful Városliget (City) Park.
The host, Balint was very accommodating towards some flight delay issues we had on the day of arrival and his mother was at the apartment to greet us when we finally arrived.
Thank you
Even though our time in Budapest was shorter than planned - Balint's apartment was absolutely perfect for our needs. Very authentic and close to Keleti train station with metro etc., clean and charming rooms, caring host. Totally recommended!
Nice apartment in an old house with high ceilings and a convenient location. Disadvantages: noisy, not enough kitchen utensils (such as frying pans). The owner is very friendly.
The apartement was great for our needs. There was enough space for 3 people and the beds were very comfortable. There was even a small courtyard, where you can sit and chill.
The location was very central, close to the trainstation, the subway and the (night) busstop was right in front of our door.
Since the apartement is central, you do have city noises, but it didn't bother us.
Balint was very helpful, friendly and easy-going. He welcomed us on the first day, gave tips and offered us to contact him anytime during our stay if we needed help.
The day we left he came by to say goodbye and to make sure everything was ok during our stay.
I would return to the apartement anytime! Everthing was fine.
Balint was very nice because we waited for us until very late because of the flight. The flat is in a building with a lot of charme. Internet works very good and the locatation very good with a park, the baths close.
Everything was really fine, and we had a nice stay in Budapest - and also a nice conversation with Balint's mother ;)
Balint is very kind and polite. Everything has been great! The house is near to a metro station and there are many and frequent ways to reach the city centre. Our staying at Balint house has been great and perfect: I advice you to consider his house if you are going to Budapest. If I go newly there, of course I will stay at Balint house.
Bell incantevole Budapest siamo stati ospiti del gentilissimo Balint! Appartamento un po datato ma spazioso e comunque centrale. Ben servito da bus sia diurni che notturni, fermata della metro nn dista molto anche se con buona volontà la città si gira bene a piedi. Ottima comunicazione con il propriietario che al nostro arrivo ci ha dato utili suggerimenti. Buon rapporto qualità -prezzo.consiglio vivamente.
- Kvalitetssikret innkvartering
- Hver vert er verifisert via telefon
- Sikker betaling med SSL-kryptering


text:
Originally posted on Pro Sports Daily | Last updated 7/18/13
The 76ers' search for a coach continues to percolate. The team interviewed Boston Celtics assistant Jay Larranaga on Tuesday, Yahoo Sports reported. The Sixers are also reportedly going to interview Atlanta Hawks assistants Quin Snyder and Kenny Atkinson for the job vacated by Doug Collins in April. Larranaga, whose father, Jim Larranaga, is the University of Miami coach, was considered a candidate for the Celtics coaching job before Brad Stevens was hired as Doc Rivers' replacement. The 38-year-old was the coach of the Erie BayHawks for the NBA Development League for two seasons before joining the Celtics staff. Larranaga also had a two-year stint as the Irish national team coach. Snyder is familiar with the Sixers, having served as their player development coach during the 2010-11 season. New Hawks coach Mike Budenholzer hired the 46-year-old as an assistant coach on June 10. Snyder spent last season as an assistant coach for CSKA Moscow of the Russian Professional Basketball League. He also was an assistant coach with the Los Angeles Lakes (2011-12), the Los Angeles Clippers (1992-93), and Duke (1995-99). He had head coaching stints with the University of Missouri (1999-2006) and the Austin Toros of the D-League (2007-10). Atkinson, 46, was hired by the Hawks last summer and retained by Budenholzer. He went to Atlanta after spending four seasons as a New York Knicks assistant. Atkinson was the Houston Rockets' player development coach during the 2008-09 season. Before that, he spent two seasons as the player development coach for Paris Basket Racing Club in France. This news comes one day after it was reported that the Sixers plan to interview Chicago Bulls assistants Ed Pinckney and Adrian Griffin for the job.
One Gotta Go: NFL Legends (and Jose Canseco!) Have No Love For Captain America
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Lentil and Spinach Soup
Cold Sesame Noodle Salad
Energy Juice Drink
Flourless Orange-Almond Cake with Mocha Sauce
Japanese Teriyaki Marinade
Steamed Salmon with Sesame Ginger Dressing
Honey Glazed Grilled Pork Chops
Hawaiian Chicken Wings
General Tso Chicken
Chicken in Avocado and Bacon Sauce
Marinade for Rib Eye Steaks
Carrot Cupcakes with Ginger-Cream Cheese Icing
Seafood Pan-Fried Noodles
Shrimp Won Ton with Hot And Sour Sauce
Paella (Spanish Chicken, Seafood Casserole)
Japanese Potstickers (gyoza)
Asian Tuna Steaks
Maple soy glazed salmon
Spicy Stir-Fried Tofu with Coconut Rice
Lumpia (Philippine Egg Rolls From Scratch)
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