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Publications (78)582.24 Total impact
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ABSTRACT: Background Chikungunya virus—a mosquito-borne alphavirus—is endemic in Africa and south and southeast Asia and has recently emerged in the Caribbean. No drugs or vaccines are available for treatment or prevention. We aimed to assess the safety, tolerability, and immunogenicity of a new candidate vaccine. Methods VRC 311 was a phase 1, dose-escalation, open-label clinical trial of a virus-like particle (VLP) chikungunya virus vaccine, VRC-CHKVLP059-00-VP, in healthy adults aged 18–50 years who were enrolled at the National Institutes of Health Clinical Center (Bethesda, MD, USA). Participants were assigned to sequential dose level groups to receive vaccinations at 10 μg, 20 μg, or 40 μg on weeks 0, 4, and 20, with follow-up for 44 weeks after enrolment. The primary endpoints were safety and tolerability of the vaccine. Secondary endpoints were chikungunya virus-specific immune responses assessed by ELISA and neutralising antibody assays. This trial is registered with ClinicalTrials.gov, NCT01489358. Findings 25 participants were enrolled from Dec 12, 2011, to March 22, 2012, into the three dosage groups: 10 μg (n=5), 20 μg (n=10), and 40 μg (n=10). The protocol was completed by all five participants at the 10 μg dose, all ten participants at the 20 μg dose, and eight of ten participants at the 40 μg dose; non-completions were for personal circumstances unrelated to adverse events. 73 vaccinations were administered. All injections were well tolerated, with no serious adverse events reported. Neutralising antibodies were detected in all dose groups after the second vaccination (geometric mean titres of the half maximum inhibitory concentration: 2688 in the 10 μg group, 1775 in the 20 μg group, and 7246 in the 40 μg group), and a significant boost occurred after the third vaccination in all dose groups (10 μg group p=0·0197, 20 μg group p<0·0001, and 40 μg group p<0·0001). 4 weeks after the third vaccination, the geometric mean titres of the half maximum inhibitory concentration were 8745 for the 10 μg group, 4525 for the 20 μg group, and 5390 for the 40 μg group. Interpretation The chikungunya VLP vaccine was immunogenic, safe, and well tolerated. This study represents an important step in vaccine development to combat this rapidly emerging pathogen. Further studies should be done in a larger number of participants and in more diverse populations. Funding Intramural Research Program of the Vaccine Research Center, National Institute of Allergy and Infectious Diseases, and National Institutes of Health.The Lancet 12/2014; 384(9959). · 39.21 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Flaviviruses are thought to sample an ensemble of structures at equilibrium. One consequence of a structurally dynamic virion is the observed time-dependent increases in neutralization sensitivity that can occur after prolonged incubation with antibody. Differences in how virus strains "breathe" may affect epitope exposure and contribute to the underlying mechanisms of strain-dependent neutralization sensitivity. Beyond the contribution of structural dynamics, flaviviruses exist as a structurally heterogeneous population due to an inefficient virion maturation process. Herein we investigate the interplay between virion maturation and structural dynamics that contribute to antibody-mediated neutralization. Using West Nile (WNV) and dengue (DENV) viruses produced under conditions that modify the extent of virion maturation, we investigated time-dependent changes in neutralization sensitivity associated with structural dynamics. Our results identify distinct patterns of neutralization against viruses that vary markedly with respect to the extent of virion maturation. Reducing the efficiency of virion maturation resulted in greater time-dependent changes in neutralization potency and a marked reduction in the stability of the particle at 37°C as compared to more mature virus. That neutralization sensitivity of WNV and DENV did not increase after prolonged incubation in the absence of antibody, regardless of virion maturation, suggests that the dynamic processes that govern epitope accessibility on infectious viruses are reversible. Against the backdrop of heterogeneous flavivirus structures, differences in the pathways by which viruses "breathe" represent an additional layer of complexity towards understanding maturation state-dependent patterns of antibody recognition.Journal of Virology 07/2014; · 4.65 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: The production of neutralizing antibodies (nAbs) is a correlate of protection for many human vaccines, including currently licensed vaccines against flaviviruses. nAbs are typically measured using a plaque reduction neutralization test (PRNT). Despite its extensive use, parameters that impact the performance of the PRNT have not been investigated from a mechanistic perspective. The results of a recent phase IIb clinical trial of a tetravalent dengue virus (DENV) vaccine suggests that nAbs, as measured using a PRNT performed with Vero cells, do not correlate with protection. This surprising finding highlights the importance of understanding how well the PRNT captures the complexity of the nAb response to DENV. In this study, we demonstrate that the structural heterogeneity of flaviviruses arising from inefficient virion maturation impacts the results of neutralization assays in a cell type-dependent manner. Neutralization titers of several monoclonal antibodies were significantly reduced when assayed on Vero cells, as compared to Raji cells expressing DC-SIGNR. This pattern can be explained by differences in the efficiency with which partially mature flaviviruses attach to each cell type, rather than a differential capacity of antibody to block infection. Vero cells are poorly permissive to the fraction of virions that are most sensitive to neutralization. Analysis of sera from recipients of live-attenuated monovalent DENV vaccine candidates revealed a strong correlation between the sensitivity of serum antibodies to the maturation state of DENV and cell type-dependent patterns of neutralization. Cross-reactive patterns of neutralization may be under-represented by the "gold-standard" PRNT that employs Vero cells. Cell type-dependent patterns of neutralization describes a differential capacity of antibodies to inhibit virus infection when assayed on multiple cellular substrates. In this study, we establish a link between antibodies that neutralize infection in a cell type-dependent fashion and those sensitive to the maturation state of the flavivirus virion. We demonstrate that cell type-dependent neutralization reflects a differential capacity to measure neutralization of viruses that are incompletely mature. Partially mature virions that most efficiently bind maturation state-sensitive antibodies are poorly represented by assays typically used in support of flavivirus vaccine development. The selection of cellular substrate for neutralization assays may significantly impact evaluation of the neutralization potency of the polyclonal response. These data suggest that current assays do not adequately capture the full complexity of the neutralizing antibody response and may hinder the identification of correlates of protection following flavivirus vaccination.Journal of Virology 04/2014; · 4.65 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: We recently described our most potently neutralizing monoclonal antibody, E106, which protected against lethal Dengue virus type 1 (DENV-1) infection in mice. To further understand its functional properties, we determined the crystal structure of E106 Fab in complex with domain III (DIII) of DENV-1 envelope (E) protein to 2.45 Å resolution. Analysis of the complex revealed a small antibody-antigen interface with the epitope on DIII composed of nine residues along the lateral ridge and A-strand regions. Despite strong virus neutralizing activity of E106 IgG at picomolar concentrations, E106 Fab exhibited a ∼20,000-fold decrease in virus neutralization and bound isolated DIII, E, or viral particles with only a micromolar monovalent affinity. In comparison, E106 IgG bound DENV-1 virions with nanomolar avidity. The E106 epitope appears readily accessible on virions, as neutralization was largely temperature-independent. Collectively, our data suggest that E106 neutralizes DENV-1 infection through bivalent engagement of adjacent DIII subunits on a single virion. The isolation of anti-flavivirus antibodies that require bivalent binding to inhibit infection efficiently may be a rare event due to the unique icosahedral arrangement of envelope proteins on the virion surface.PLoS Pathogens 04/2014; 10(4):e1004072. · 8.06 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: This chapter outlines methods for the production of dengue virus (DENV) reporter virus particles (RVPs) and their use in assays that measure antibody-mediated neutralization and enhancement of DENV infection. RVPs are pseudo-infectious virions produced by complementation of a self-replicating flavivirus replicon with the DENV structural genes in trans. RVPs harvested from transfected cells are capable of only a single round of infection and encapsidate replicon RNA that encodes a reporter gene used to enumerate infected cells. RVPs may be produced using the structural genes of different DENV serotypes, genotypes, and mutants by changing plasmids used for complementation. Further modifications are possible including generating RVPs with varying levels of uncleaved prM protein, which resemble either the immature or mature form of the virus. Neutralization potency is measured by incubating RVPs with serial dilutions of antibody, followed by infection of target cells that express DENV attachment factors. Enhancement of infection is measured similarly using Fc receptor-expressing cells capable of internalizing antibody-virus complexes.Methods in molecular biology (Clifton, N.J.) 01/2014; 1138:75-97. · 1.29 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Dengue viruses are mosquito-borne flaviviruses that circulate in nature as four distinct serotypes (DENV1-4). These emerging pathogens are responsible for more than 100 million human infections annually. Severe clinical manifestations of disease are predominantly associated with a secondary infection by a heterotypic DENV serotype. The increased risk of severe disease in DENV-sensitized populations significantly complicates vaccine development, as a vaccine must simultaneously confer protection against all four DENV serotypes. Eliciting a protective tetravalent neutralizing antibody response is a major goal of ongoing vaccine development efforts. However, a recent large clinical trial of a candidate live-attenuated DENV vaccine revealed low protective efficacy despite eliciting a neutralizing antibody response, highlighting the need for a better understanding of the humoral immune response against dengue infection. In this study, we sought to identify epitopes recognized by serotype-specific neutralizing antibodies elicited by monovalent DENV1 vaccination. We constructed a panel of over 50 DENV1 structural gene variants containing substitutions at surface-accessible residues of the envelope (E) protein to match the corresponding DENV2 sequence. Amino acids that contribute to recognition by serotype-specific neutralizing antibodies were identified as DENV mutants with reduced sensitivity to neutralization by DENV1 immune sera, but not cross-reactive neutralizing antibodies elicited by DENV2 vaccination. We identified two mutations (E126K and E157K) that contribute significantly to type-specific recognition by polyclonal DENV1 immune sera. Longitudinal and cross-sectional analysis of sera from 24 participants of a phase I clinical study revealed a markedly reduced capacity to neutralize a E126K/E157K DENV1 variant. Sera from 77% of subjects recognized the E126K/E157K DENV1 variant and DENV2 equivalently (<3-fold difference). These data indicate the type-specific component of the DENV1 neutralizing antibody response to vaccination is strikingly focused on just two amino acids of the E protein. This study provides an important step towards deconvoluting the functional complexity of DENV serology following vaccination.PLoS Pathogens 12/2013; 9(12):e1003761. · 8.06 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Flavivirus-infected cells secrete a structurally heterogeneous population of viruses because of an inefficient virion maturation process. Flaviviruses assemble as non-infectious, immature virions composed of trimers of envelope (E) and precursor-membrane (prM) protein heterodimers. Cleavage of prM is a required process during virion maturation, although this often remains incomplete on infectious virus particles. Previous work demonstrated that the efficiency of virion maturation could impact antibody neutralization through changes in the accessibility of otherwise cryptic epitopes on the virion. In this study, we show that the neutralization potency of monoclonal antibody (mAb) E33 is sensitive to the maturation state of West Nile virus (WNV) despite recognizing an accessible epitope, the domain III lateral ridge (DIII-LR). Comprehensive epitope mapping studies using 166 E protein DIII-LR variants revealed that the functional footprint of mAb E33 on the E protein differs subtly from that of the well characterized DIII-LR mAb E16. Remarkably, aromatic substitutions at E protein residue 306 ablated the maturation-state sensitivity of E33 IgG, and the neutralization efficacy of E33 Fab fragments was not affected by changes in the virion maturation state. We propose that E33 IgG binding on mature virions orients the Fc region in a manner that impacts subsequent antibody binding to nearby sites. This Fc-mediated steric constraint is a novel mechanism by which the maturation state of a virion modulates the efficacy of the humoral immune response to flavivirus infection.Journal of Virology 10/2013; · 4.65 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Dengue virus (DENV) is a mosquito-transmitted flavivirus that can cause severe disease in humans and is considered a re-emerging pathogen of significant importance to public health. The DENV capsid (C) protein functions as a structural component of the infectious virion, however, it may have additional functions in the virus replicative cycle. Here we show that the DENV C protein interacts and co-localizes with the multi-functional host protein nucleolin (NCL). Furthermore, we demonstrate that this interaction can be disrupted by the addition of a NCL binding aptamer (AS1411). Knockdown of NCL with siRNA, or treatment of cells with AS1411 results in a significant reduction of viral titers after DENV infection. Western blot and qRT-PCR analysis revealed no differences in viral RNA or protein levels at early time points post-infection, suggesting a role for NCL in viral morphogenesis. We support this hypothesis by showing that treatment with AS1411 alters the migration characteristics of the viral capsid as visualized by native electrophoresis. Here we identify a critical interaction between DENV C protein and NCL that represents a potential new target for the development of antiviral therapeutics.Journal of Virology 09/2013; · 4.65 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Although prior studies have characterized the neutralizing activity of monoclonal antibodies (MAbs) against DENV serotypes 1, 2, and 3 (DENV-1, DENV-2, and DENV-3), few reports have assessed the activity of MAbs against DENV-4. Here, we evaluated the inhibitory activity of 81 new mouse anti-DENV-4 MAbs. We observed strain and genotype-dependent differences in neutralization of DENV-4 by MAbs mapping to epitopes on domains II (DII) and III (DIII) of the envelope (E) protein. Several anti-DENV-4 MAbs inefficiently inhibited at least one strain and/or genotype, suggesting that the exposure or sequence of neutralizing epitopes varies within isolates of this serotype. Remarkably, flavivirus cross-reactive MAbs, which bound to the highly conserved fusion loop in DII and inhibited infection of DENV-1, DENV-2, and DENV-3, more weakly neutralized five different DENV-4 strains encompassing the genetic diversity of the serotype after pre-incubation at 37°C. However, increasing the time of pre-incubation at 37°C or raising the temperature to 40°C enhanced the potency of DII fusion loop-specific MAbs and some DIII-specific MAbs against DENV-4 strains. Prophylaxis studies in two new DENV-4 mouse models showed that neutralization titers of MAbs after pre-incubation at 37°C correlated with activity in vivo. Our studies establish the complexity of MAb recognition against DENV-4, and suggest that differences in epitope exposure relative to other DENV serotypes affects antibody neutralization and protective activity.Journal of Virology 06/2013; · 4.65 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus that causes global epidemics of a debilitating polyarthritis in humans. As there is a pressing need for the development of therapeutic agents, we screened 230 new mouse anti-CHIKV monoclonal antibodies (MAbs) for their ability to inhibit infection of all three CHIKV genotypes. Four of 36 neutralizing MAbs (CHK-102, CHK-152, CHK-166, and CHK-263) provided complete protection against lethality as prophylaxis in highly susceptible immunocompromised mice lacking the type I IFN receptor (Ifnar(-/-) ) and mapped to distinct epitopes on the E1 and E2 structural proteins. CHK-152, the most protective MAb, was humanized, shown to block viral fusion, and require Fc effector function for optimal activity in vivo. In post-exposure therapeutic trials, administration of a single dose of a combination of two neutralizing MAbs (CHK-102+CHK-152 or CHK-166+CHK-152) limited the development of resistance and protected immunocompromised mice against disease when given 24 to 36 hours before CHIKV-induced death. Selected pairs of highly neutralizing MAbs may be a promising treatment option for CHIKV in humans.PLoS Pathogens 04/2013; 9(4):e1003312. · 8.06 Impact Factor -
Article: Flaviviruses: braking the entering.
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ABSTRACT: Flaviviruses are small spherical virus particles covered by a dense icosahedral array of envelope (E) proteins that mediate virus attachment to cells and the fusion of viral and cellular membranes. Our understanding of the mechanism by which flavivirus E proteins orchestrate entry into cells has been advanced by studies of E structure and arrangement on the virion at different steps of the virus entry/membrane fusion process. When combined with an increasingly clear (albeit still incomplete) view of the cell biology of virus entry, these advances suggest new antiviral strategies. Indeed, inhibitors that target cellular and viral processes involved in entry show promise as powerful tools to study this critical step of the viral lifecycle, and with luck, may ultimately lead to therapeutic advances.Current opinion in virology. 01/2013; -
Article: Impact of viral attachment factor expression on antibody-mediated neutralization of flaviviruses.
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ABSTRACT: Neutralization of flaviviruses requires engagement of the virion by antibodies with a stoichiometry that exceeds a required threshold. Factors that modulate the number of antibodies bound to an individual virion when it contacts target cells impact neutralization potency. However, the contribution of cellular factors to the potency of neutralizing antibodies has not been explored systematically. Here we investigate the relationship between expression level of a viral attachment factor on cells and the neutralizing potency of antibodies. Analysis of the attachment factor DC-SIGNR on cells in neutralization studies failed to identify a correlation between DC-SIGNR expression and antibody-mediated protection. Furthermore, neutralization potency was equivalent on a novel Jurkat cell line induced to express DC-SIGNR at varying levels. Finally, blocking virus-attachment factor interactions had no impact on neutralization activity. Altogether, our studies suggest that cellular attachment factor expression is not a significant contributor to the potency of neutralizing antibodies to flaviviruses.Virology 01/2013; · 3.28 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: We previously developed a panel of neutralizing monoclonal antibodies against Dengue virus (DENV)-1, of which few exhibited inhibitory activity against all DENV-1 genotypes. This finding is consistent with reports observing variable neutralization of different DENV strains and genotypes using serum from individuals that experienced natural infection or immunization. Herein, we describe the crystal structures of DENV1-E111 bound to a novel CC' loop epitope on domain III (DIII) of the E protein from two different DENV-1 genotypes. Docking of our structure onto the available cryo-electron microscopy models of DENV virions revealed that the DENV1-E111 epitope was inaccessible, suggesting that this antibody recognizes an uncharacterized virus conformation. While the affinity of binding between DENV1-E111 and DIII varied by genotype, we observed limited correlation with inhibitory activity. Instead, our results support the conclusion that potent neutralization depends on genotype-dependent exposure of the CC' loop epitope. These findings establish new structural complexity of the DENV virion, which may be relevant for the choice of DENV strain for induction or analysis of neutralizing antibodies in the context of vaccine development.PLoS Pathogens 10/2012; 8(10):e1002930. · 8.06 Impact Factor -
Article: Measles immunometrics.
Proceedings of the National Academy of Sciences 08/2012; 109(37):14724-5. · 9.81 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Flaviviruses are small enveloped virions that enter target cells in a pH-dependent fashion. Virus attachment, entry, and membrane fusion are orchestrated by the envelope (E) and pre-membrane (prM) proteins, the two structural proteins displayed on the surface of virions. Flaviviruses assemble as an immature non-infectious form onto which prM and E form trimeric spikes. During egress from infected cells, flaviviruses undergo dramatic structural changes characterized by the formation of a herringbone arrangement of E proteins that lie flat against the surface of the virion and cleavage of the prM protein by the cellular protease furin. The result is a relatively smooth, infectious mature virion. This dynamic process is now understood in structural detail at the atomic level. However, recent studies indicate that many of the virions released from cells share structural features of both immature and mature virus particles. These mosaic partially mature virions are infectious and interact uniquely with target cells and the host immune response. Here, we will discuss recent advances in our understanding of the biology and significance of partially mature flaviviruses.Current opinion in virology. 03/2012; 2(2):168-75. - [Show abstract] [Hide abstract]
ABSTRACT: DENV1-E106 is a monoclonal antibody (MAb) with strong neutralizing activity against all five DENV-1 genotypes and therapeutic activity in mice. Here, we evaluated the potential for DENV-1 to escape neutralization by DENV1-E106. A single mutation in domain III of the envelope protein (T329A) emerged, which conferred resistance to DENV1-E106. However, the T329A variant virus had differing phenotypes in vitro and in vivo with attenuation in cell culture yet increased infectivity in Aedes aegypti mosquitoes. Mice infected with this T329A variant still were protected against lethal infection by DENV1-E106 even though much of the neutralizing activity was lost. This study reveals the complex dynamics of neutralization escape of an inhibitory MAb against DENV, and suggests that evaluation of therapeutic MAbs requires detailed investigation in relevant hosts.Virology 03/2012; 427(2):127-34. · 3.28 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: The four serotypes of dengue virus present a formidable challenge for the development of efficacious human vaccines. Cockburn and colleagues, in this issue of Structure, describe the structural basis of a cross-reactive neutralizing antibody, providing greater insight into immune protection and pathogenesis.Structure 02/2012; 20(2):200-2. · 6.79 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Molecular clone technology has proven to be a powerful tool for investigating the life cycle of flaviviruses, their interactions with the host, and vaccine development. Despite the demonstrated utility of existing molecular clone strategies, the feasibility of employing these existing approaches in large-scale mutagenesis studies is limited by the technical challenges of manipulating relatively large molecular clone plasmids that can be quite unstable when propagated in bacteria. We have developed a novel strategy that provides an extremely rapid approach for the introduction of mutations into the structural genes of West Nile virus (WNV). The backbone of this technology is a truncated form of the genome into which DNA fragments harboring the structural genes are ligated and transfected directly into mammalian cells, bypassing entirely the requirement for cloning in bacteria. The transfection of cells with this system results in the rapid release of WNV that achieves a high titer (∼10(7) infectious units/ml in 48 h). The suitability of this approach for large-scale mutagenesis efforts was established in two ways. First, we constructed and characterized a library of variants encoding single defined amino acid substitutions at the 92 residues of the "pr" portion of the precursor-to-membrane (prM) protein. Analysis of a subset of these variants identified a mutation that conferred resistance to neutralization by an envelope protein-specific antibody. Second, we employed this approach to accelerate the identification of mutations that allow escape from neutralizing antibodies. Populations of WNV encoding random changes in the E protein were produced in the presence of a potent monoclonal antibody, E16. Viruses resistant to neutralization were identified in a single passage. Together, we have developed a simple and rapid approach to produce infectious WNV that accelerates the process of manipulating the genome to study the structure and function of the structural genes of this important human pathogen.Journal of Virology 01/2012; 86(7):3501-12. · 4.65 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: RNA interference can be mediated by fully complementary siRNA or partially complementary miRNA. siRNAs are widely used to suppress viral replication and the fully complementary siRNA bound Ago-2 in the RISC is known to degrade the target RNA. Although other argonaute proteins lacking slicer activity can also bind oligonucleotides with both si and miRNA structures, whether they can also contribute to antiviral effects is not entirely clear. We tested si and miRNA structured oligos for target repression in dual luciferase assays as well as for inhibition of Dengue and West Nile virus replication in ES cells expressing individual Ago proteins. In luciferase assays, both fully complementary and partially complementary oligos effectively repressed their targets in all individual Ago expressing cell lines, although the efficacy with fully complementary oligos was higher in Ago-2+ cells. However, partially complementary oligos had no effect on virus replication in any cell line, while fully complementary siRNAs were highly effective in Ago-2 expressing, but not in cells expressing other Ago proteins. This occurred irrespective of whether the target sequences were located in the coding region or 3'UTR of the virus. We conclude that Ago-2 slicer activity is essential for anti-viral efficacy of siRNAs and miRNA-mediated translational repression/transcript destabilization is too weak to suppress the abundantly expressed flaviviral proteins.PLoS ONE 11/2011; 6(11):e27551. · 3.53 Impact FactorThis article is viewable in ResearchGate's enriched format - [Show abstract] [Hide abstract]
ABSTRACT: The human antibody response to flavivirus infection is dominantly directed against a cross-reactive epitope on the fusion loop of domain II (DII-FL) of the envelope (E) protein. Although antibodies against this epitope fail to recognize fully mature West Nile virus (WNV) virions and accordingly neutralize infection poorly in vitro, their functional properties in vivo remain less well understood. Here, we show that while passive transfer of poorly neutralizing monoclonal antibodies (MAb) and polyclonal antibodies against the DII-FL epitope protect against lethal WNV infection in wild-type mice, they fail to protect mice lacking activating Fcγ receptors (FcγR) and the complement opsonin C1q. Consistent with this, an aglycosyl chimeric mouse-human DII-FL MAb (E28) variant that lacks the ability to engage FcγR and C1q also did not protect against WNV infection in wild-type mice. Using a series of immunodeficient mice and antibody depletions of individual immune cell populations, we demonstrate that the nonneutralizing DII-FL MAb E28 does not require T, B, or NK cells, inflammatory monocytes, or neutrophils for protection. Rather, E28 treatment decreased viral load in the serum early in the course of infection, which resulted in blunted dissemination to the brain, an effect that required phagocytic cells, C1q, and FcγRIII (CD16). Overall, these studies enhance our understanding of the functional significance of immunodominant, poorly neutralizing antibodies in the polyclonal human anti-flavivirus response and highlight the limitations of current in vitro surrogate markers of protection, such as cell-based neutralization assays, which cannot account for the beneficial effects conferred by these antibodies.Journal of Virology 09/2011; 85(22):11567-80. · 4.65 Impact Factor
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This story is about how two bestfriends fall in love with each other and all the drama and happiness the relationship will bring :)
a month ago
What if that big ass suitcase dropped on your foot girl. You gonna cry and imma laugh.
7 months ago
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she is to damn dramatic shit doing diggy like that you dumb hoe he likes you.
7 months ago
what was he doing in her room anyway are adopted sister and brother like i don't even know how old they are.
7 months ago
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why the fuck she acting like that it's to damn early in the story for her to have trashy ass attitude like chill its really to early for all that shit that's to much for a first chapter.

subset: commoncrawl


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The Book Club is open to the entire Einstein Community. Meetings are held every other month. Books are selected by club members. If you have any questions please contact the Reference Department at email@example.com or 718-430-3104.
This year our theme is food. Join us at our next meeting!
DATE: Wednesday, March 11, 2015
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BOOK: The Botany of Desire: A Plants-Eye View of the World, by Michael Pollan
"Pollan shines a light on our own nature as well as our implication in the natural world."
– The New York Times
The Book Club has an email list for discussion and announcements. Sign up at https://lists.aecom.yu.edu/mailman/listinfo/bookclub.


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Munhoz, Alexandre Mendonça M.D.; Aldrighi, Claudia M.D.; Montag, Eduardo M.D.; Arruda, Eduardo Gustavo M.D.; Aldrighi, José Mendes M.D., Ph.D.; Filassi, José Roberto M.D., Ph.D.; Ferreira, Marcus Castro M.D., Ph.D.
Background: Although use of the latissimus dorsi myocutaneous flap associated with the Biodimensional anatomical expander implant system (McGhan 150) is a reliable technique, little information has been available regarding clinical outcome following periareolar skin-sparing mastectomy reconstruction. The purpose of this study was to analyze the feasibility of the technique, surgical planning, and its outcome following skin-sparing mastectomy.
Methods: Thirty-two patients underwent immediate unilateral latissimus dorsi myocutaneous flap/Biodimensional anatomical expander implant system breast reconstruction. Mean follow-up was 18 months. The technique was indicated in patients with small- or moderate-volume breasts with or without ptosis, in whom the use of abdominal flaps was precluded. Flap and donor-site complications were evaluated. Information on anesthetic results and patient satisfaction was collected.
Results: Seventy-two percent had tumors measuring 2 cm or less (T1) and 78 percent were stage 0 and I according to American Joint Committee on Cancer criteria. Breast skin complications occurred in 9.4 percent. Two patients presented small breast skin necrosis, and in one patient, a wound dehiscence was observed. Donor-site complications, all represented by seroma, occurred in 12.5 percent. The cosmetic result was considered good or very good in 84.4 percent, and the majority of patients were either very satisfied or satisfied. No local recurrences were observed. All complications except two were treated by conservative means.
Conclusions: The latissimus dorsi myocutaneous flap/Biodimensional anatomical expander implant system is a simple and reliable technique for periareolar skin-sparing mastectomy reconstruction. Success depends on patient selection, coordinated planning with the oncologic surgeon, and careful intraoperative and postoperative management.
©2007American Society of Plastic Surgeons
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The Clinical Masters of PRS – Breast eBooks 4 Essential eBooks for Plastic Surgeons


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Everybody knows John 3:16 but nobody goes around wearing it. Its a great conversation starter!
This great custom product in black from our department Women and T-Shirt category can be printed just for you in the size and style you like. The design J316 was created by VThreads and uploaded to Spreadshirt for you to have printed on a T-Shirt. Next to a host of other great customized designs, you can find this J316 design in Spreadshirt's Christianity category. If you are interested in topics like God or Jesus, then you'll love J316 designs by VThreads. Spreadshirt also offers a huge selection of custom t-shirts, hoodies and accessories for the category Christianity as well as top christian clothing. VThreads's designs have already impressed many of our customers, so we are sure that we've got right stuff for you! Spreadshirt is your destination for great designs of all kinds – whether it's God, Jesus or christian. We are sure that you will find something nice in our shop, there are many amazing custom Christianity t-shirts, hoodies, or accessories for you to find! Tags: Design ID: 12476790
DesignsbyFaith is a Christian apparel idea with charity as the foundation. The backbone can be found in Acts 20:35.. I have shewed you all things, how that so labouring ye ought to support the weak, and to remember the words of the Lord Jesus, how he said, 'It is more blessed to give than to receive.' VThreads offers epic text based graphic apparel for a new generation thats both inspiring and creative.
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This figure-flattering, slim fit t-shirt is soft, stylish and above all comfortable. This is the one you don't let your girlfriends borrow. It's perfect with jeans, khakis, leggings or pretty much any other bottom and is great for layering.


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Nonprofit health providers find financing |
| Print | |
Must Reads | |||
Wednesday, July 25, 2012 | |||
Despite financial uncertainty, Portland nonprofit health systems keep finding ways to finance ambitious projects. Read more at OregonLive.com. {biztweet}portland health{/biztweet}
VIDEO: The 2015 100 Best Companies to Work For in OregonThe LatestFriday, February 27, 2015 VIDEO: 2015 100 Best Companies to work for in Oregon Read more... |
10 Twitter highlights from #OR100BestThe LatestFriday, February 27, 2015 BY OB STAFFOregon Business held its 22nd annual 100 Best Companies to Work For in Oregon celebration Thursday night in the Oregon Convention Center. Read more... |
Game OnMarch 2015Wednesday, February 25, 2015 BY LINDA BAKER | OB EDITORThe big news at Oregon Business is we're getting a ping pong table. After reading the descriptions of the 2015 100 Best Companies to Work For in Oregon, a disproportionate number of which feature table tennis in the office, I decided it was time to bring our own workplace into the 21st century. It was a tough call, but it's lonely at the top, and someone has to make the hard decisions. Read more... |
Money TalksMarch 2015Saturday, February 21, 2015 BY LINDA BAKER | OB DIGITAL NEWS EDITORWill community banks survive the digital age? Three CEOs peer into banking's crystal ball. Read more... |
That's Not a Watch (This Is a Watch)February 2015Tuesday, January 27, 2015 Smartwatches are all the rage. But old-fashioned timepieces keep on ticking. Read more... |
Finding a BalanceContributed BlogsThursday, January 29, 2015 BY JASON NORRIS | OB GUEST BLOGGERActive vs. passive investing: what you need to know. Read more... |
All RiseMarch 2015Friday, February 20, 2015 BY AMY MILSHTEIN | OB CONTRIBUTORDon't just sit there. For a healthy workplace, move up and down — and all around. Read more... |
Real Time - Oregon Business
TWITTER FEED Tweets by @OregonBusiness |
Generations of students and graduates have been plagued by the question: What is my true calling in life? Four alumni from Corban University's Hoff School of Business who graduated in different decades say the school helped them find the answer by giving them a practical, well-rounded education.
It's happening whether anyone's ready or not. Businesses here in Oregon and across the U.S. are already experiencing the effects of the largest generational shift in recent history, and these changing tides will impact every level of the workplace — from a company's executive leadership to its cultural core.
Success stories spotlight meaningful career opportunities in Oregon's diverse and lucrative tourism industry.
The Firm was recognized for the strength of its case matters during 2014, including precedents set or verdicts with notable high dollar amounts at stake.
The Oregon Chapter of the Society for Marketing Professional Services, will be hosting it's Annual Dinner and Keynote event on March 12, 2015. The evening promises to be memorable, with this years Keynote, Christine McKinley.
Lane Powell will team with Oregon Business magazine for a half-day seminar titled "Best Practices For Best Employers™: How to Become One of 'Oregon's Best Workplaces' Starting Today!"


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Definition of asserter
n. - One who asserts; one who avers pr maintains; an assertor.
The word "asserter" uses 8 letters: A E E R R S S T.
Direct anagrams of asserter:
reassert serrates terrases
Words formed by adding one letter before or after asserter (in bold), or to aeerrsst in any order:
e - arrestees k - streakers m - remasters streamers r - arresters rearrests s - asserters reasserts u - treasures v - traverses
List all words starting with asserter, words containing asserter or words ending with asserter
All words formed from asserter by changing one letter
Other words with the same letter pairs: as ss se er rt te er
Browse words starting with asserter by next letter
Previous word in list: assertedly
Next word in list: asserters
Some random words: gharial ocrea huarache djin niacin oogamete much
This is not a dictionary, it's a word game wordfinder.
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"I just ordered these for my baby shower, I'm having a boy, and they came out wonderful! I ordered them on the signature matte paper and they are bright and cute! I would definitely recommend these invitations and will be ordering from here again!"
"Absolutely loved this product. Very professional. Appreciated them double-checking for alignment, spelling errors, etc! Arrived when it said it would! Only part I was disappointed with was the envelope. Unfortunately you could see right through the envelope to the invitation. But I did appreciate that they offer an envelope to decrease on postage costs!"
"Great company, invites came in 2 days! They are even prettier in person. We needed to be able to include a lot of information on the invite and I was worried it might look crowded. It did not! Getting lots of compliments on them too!"
"Ive received many compliments on this card. The paper quality is excellent, the design creative. You can choose your desired font, colors, & borders. Very well made and delivered quickly after ordering!"
"The staff was especially helpful personalizing the text. The finished product was on excellent quality paper and was pleasing to everyone involved in the party planning."


text:
When you listen to Anime soundtracks, which songs can you really, god how do i say this? (thinking...) which songs can you just keep listening to over and over, that just keep you in one mood, and you can't change it? :D
There are a couple for me:
1. Ningyo Hime, by Tanaka Rei, from Chobits
2. Key of the Twilight, from .hack//twilight
3. mostly the rest of the Chobits songs, ^.^
:thumb: :thumb: :thumb:


text:
Music Lyrics Bass and Guitar Tabs and More... |
This page has been accessed times. This page is updated frequently!! Return to my music page or my home page The creator of this page is in no way affiliated with Stroke 9. The creator of this page is simply a fan that made a page about them. Website © 2000-2014 mattsmusicpage.com. All rights reserved. Legal & DMCA: Matt's Music Page has been providing lyrics and tabs in a legal way, paying royalties each year to a few organizations. Then, all lyrics and tabs were removed from the site. Now, there's a new owner to the site, which purchased it August 26, 2014, and he is in negotiation to obtain licensing from BMI at the moment. If you have a specific DMCA request -- please do not hesitate to contact me via the contact button on top. I will do my very best to keep this site fully legal. |
VINTAGE Christmas mice MOUSE band MUSIC BOX display FOLK ART guitar drums UNIQUE 1976 FYFE & DRUM EXTRA LYTE BEER GENESEE BREWING CO ROCHESTER NY 6-PACK CASE New Christmas Ornament: or Cake Topper: Sesame Street Elmo beating Drum LOOK Estate Christmas Ornament :Hard Plastic, Soldier Playing Drum Adorable LOOK Estate Christmas Ornament: 3-1/2" Tall Winnie the Pooh Playing the Drums LOOK |


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Thought Bubbles
Its too warm to do anything today... Fourth floor apartment with no a/c sucks!!
September 6, 2011 at 5:58 PM
Jacob and Israel were born Dec. 4th 2010. They are my whole world! I can't wait to be able to bring them home from the hospital.
December 9, 2010 at 9:38 PM
1-5 of 5 Thought Bubbles


text:
[Verse 1: Frank Ocean]
And the peaches and the mangos
You could sell for me
What do you think my brain is made for
Is it just a container for the mind
This great grey matter
Sensei replied what is your woman
Is she just a container for the child
That soft pink matter
Cotton candy Majin Buu
Close my eyes and fall into you
My god she's giving me pleasure
What if the sky and the stars are for show
And the aliens are watching live
From the purple matter
Sensei went quiet then violent
And we sparred until we both grew tired
Nothing mattered
Cotton candy Majin Buu
Dim the lights and fall into you
My god giving me pleasure
Pleasure pleasure pleasure
Pleasure over matter
[Verse 2: Andre 3000]
Since you been gone
I been having withdrawals
You were such a habit to call
I ain't myself at all had to tell myself naw
She's better with some fella with a regular job
I didn't wanna get her involved
By dinner Mr. Benjamin was sitting in awe
Hopped into my car; drove far
Far's too close and I remember
My memories no sharp
Butter knife, what a life, anyway
I'm building y'all a clock stop
What am I Hemingway
She had the kind of body
That would probably intimidate
Any of 'em that were un-southern
Not me cousin
If models are made for modeling
Thick girls are made for cuddlin'
Switch worlds and we can huddle then
Who needs another friend
I need to hold your hand
You'd need no other man
We'd flee to other lands
Grey matter
Blue used to be my favorite color
Now I ain't got no choice
Blue matter
You're good at being bad
You're bad at being good
For heaven's sakes go to hell
Nah knock on wood
You're good at being bad
You're bad at being good
For heaven's sakes go to hell
Knock knock knock knock on wood
Well frankly when that ocean so motherfucking good
Make her swab the motherfucking wood
Make her walk the motherfucking plank
Make her rob a motherfucking bank
With no mask on and a rusty revolver
Writer(s): James Ryan Wuihun Ho, James Ryan Ho, Andre Benjamin, Christopher Breaux
Copyright: Bug Music, Chrysalis Music, Bughouse, Heavens Research, Wedontplayevenwhenwereplayinsongs LLC, Wedontplayevenwhenwereplayin Songs LLC, In Thee Face Music Publishing
Lyrics powered by www.musiXmatch.com
1 | Lost |
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4 | Fertilizer |
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6 | Sweet Life |
7 | Not Just Money |
8 | Super Rich Kids |
9 | Pilot Jones |
10 | Crack Rock |
11 | Pyramids |
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17 | End/Golden Girl |
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Frank Ocean - Channel ORANGE Album Lyrics | |
1. | Lost |
2. | Start |
3. | Thinkin Bout You |
4. | Fertilizer |
5. | Sierra Leone |
6. | Sweet Life |
7. | Not Just Money |
8. | Super Rich Kids |
9. | Pilot Jones |
10. | Crack Rock |
11. | Pyramids |
12. | White |
13. | Monks |
14. | Bad Religion |
15. | Pink Matter |
16. | Forrest Gump |
17. | End/Golden Girl |
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1 | Memrise |
2 | Wise Man |
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4 | Whip Appeal |
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The X Factor (Season 2)
'The X Factor' behind-the-scenes: Exclusive details you didn't see on last night's premiere
By Reality TV World staff, 09/13/2012
The X Factor
's second season debut last night with coverage of the competition's auditions in Austin, San Francisco and Providence and the introductions of Britney Spears and Demi Lovato, who will join returning judges
Simon Cowell
L.A. Reid
on the show's new judging panel.
According to Cowell, Providence was one of the top stops on this summer's audition tour -- and Reality TV World was there, witnessing everything from the judges' initial arrivals to the final audition by a singer who sang The Temptations' "My Girl." (In fact, we live-tweeted coverage at the time --
follow us on Twitter
if you don't already!)
Below are some behind-the-scene details about the auditions of advancing auditioners Jennel Garcia and Jillian Jensen that you didn't get to see on television last night, as well as the Providence auditions in general.
Reality TV World: The X Factor (Season 2)
More The X Factor (Season 2) News
- While Jennel ended up being a "young Pat Benatar," she initially came across as Miley Cyrus-like to the judges and audience. Sorry Jennel!
- The judges' praise of Jennel was even more gushing than what was shown on TV and featured Demi admitting she had a girl crush on her. Some of the judges' unbroadcast comments included:
"This is what I have been waiting for today."
"I had a girl crush in Austin but I think you're my new one."
"You're probably the most promising act I've seen in this competition so far."
- Although it was emotional, the studio audience didn't have the benefit of having watched Jillian's background package that detailed the extent of her bullying prior to her audition -- making the live performance less impactful than last night's broadcast.
- Given their distance from her, Demi's tears were also not visible to the studio audience.
- Unlike home viewers, the studio audience didn't get to hear Demi's conversation with Jillian when she came up on stage and hugged Jillian after her performance -- Demi's mic audio was not piped into the sound system.
- In addition to her bullying, Jillian also mentioned her background as a member of the Varsity Girls, a local teen girl group, when she introduced herself to the judges.
- Jillian's grandma may have been even more excited about her advancement than her granddaughter was -- and she loved the crowd. What a ham!
- While their auditions aired within minutes of each other on TV, Jennel and Jillian actually auditioned on separate days. Jennel was the third audition of Day 2's evening session while Jillian was the sixth audition of Day 3's evening session. Yeah, we did count!
- Yes, Jennel and Jillian really are nearly the same age and from the same small Massachusetts town of Rochester (population 5,232 according to the 2010 U.S. Census.) Maybe there's something in the drinking water?
- Britney must have lightened up and quieted down as
The X Factor
audition tour went on, because she was not as nasty as her Austin audition editing package presented during the Providence auditions. In fact, she spoke the least of any judge and headed backstage quickly during nearly every filming break.
- While
The X Factor
Providence auditions will air over two episodes (look for more on tonight's broadcast!) they took place over three days (June 27-29) and consisted of six sessions -- three afternoon sessions and three evening sessions. Each session lasted about four hours.
- Britney, Demi, and
L.A. Reid
wore the same outfits during all six audition sessions. However if you watched closely, you'll see Simon changed his tight-fitting T-shirt from session to session, bouncing between fifty shades of gray and black.
- Viewers only saw him on TV briefly, but Britney's fiance Jason Trawick was a constant off-screen presence during the Providence auditions.
- Britney, Demi and L.A. all made individual public entrances and posed for press and fan photos on the first day of the Providence auditions, but Simon made a private entrance, avoiding the crowd. Maybe he was having a bad hair day?
- Although viewers only saw a handful of auditions on Wednesday night's premiere, over a hundred acts auditioned for the judges while they were in Providence.
(Photo credit Fox)
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Fifth Harmony member Camila Cabello and Austin Mahone split
Britney Spears confirms new romance, reveals there's a "special someone" in her life
Britney Spears signs two-year residency to perform in Las Vegas
'The X Factor' finalist Beatrice Miller signs record deal
Demi Lovato reveals she has discovered an unknown half-sister
'The Bachelor' host Chris Harrison on 'The Women Tell All:' I don't understand the hatred towards Britt Nilsson
Bobby Brown lawyer denies family is shooting a reality show based on Bobbi Kristina Brown condition
Carrie Underwood and husband Mike Fisher welcome a baby boy
'The Voice' artist India Carney wows all four coaches, Adam Levine makes her a promise
'Teen Mom' star Amber Portwood confirms engagement to boyfriend
'The Bachelor' bachelorette Britt Nilsson: Carly Waddell ruined it for me, I believe I would've won Chris Soules' heart
'The Bachelor' producer Mike Fleiss denies Kaitlyn Bristowe has been chosen as 'The Bachelorette' star
'The Bachelor's Kelsey Poe: Chris Soules and I parted ways mutually
Britney Spears
Demi Lovato
Khloe Kardashian
L.A. Reid
Mario Lopez
Simon Cowell
Jamie Dornan eats his own abs on 'The Graham Norton Show'
Lorena Rojas, Mexican actress, dies of cancer at 44
'It's My Party' singer Lesley Gore dies at 68
Three women arrested for assault at 'Fifty Shades' screening
'SNL40' -- Chevy Chase's health questioned on red carpet
Cameron Diaz and Nicole Richie double date for Valentine's Day
'Beauty and the Beast' for fourth season by The CW
Sarah Palin, Alec Baldwin hit the red carpet for the 'SNL' 40th anniversary event
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Jim GrobeJim Grobe resigning after 13 years at Wake ForestSubmitted by WWAY on Mon, 12/02/2013 - 5:29pm.READ MORE:
Wake Forest coach Jim Grobe is resigning after 13 seasons at the school.
Grobe made the announcement Monday during a news conference, saying "I just really feel right now, it's probably good for the program to have some new energy, some new direction."
Full story... » |


text:
Courtroom Insight and Daubert Tracker Announce New Partnership
I am excited to announce our new partnership with the Daubert Tracker. As a result of this agreement, Courtroom Insight now offers access to over 100,000 records detailing specific challenges to expert witness testimony. This content is organized and accessible from individual expert and judge profiles on Courtroom Insight. Initially, the challenge data is only available to law firm subscribers; however, individual subscriptions will go on sale in the near future.
If expert witness reviews and challenge information isn't enough, we also offer custom expert witness research reports that offer a comprehensive look at an expert's background, prior and pending challenges, testimonial history and more. Performing due diligence about an expert witness has never been easier!
Read the full press release here.
Happy Holidays
Decision in Ferguson Case Leads the Brown Family to Offer Solutions
A grand jury in Missouri decided on Monday not to indict Darren Wilson, a Ferguson police officer who shot and killed Michael Brown, an unarmed teenager. Brown's death has reignited the debate about law enforcement, race and power. The decision has led to riots and protests in multiple cities; people feel that justice was not served. Along with these protests, debates have started about possible ways to stop this from happening again. Michael Brown's parents have opened the discussion by asking for "a sensible change in police procedures-namely, a move to equip street officers with tiny video cameras that would record interactions with citizens, including criminal suspects." This is already taking place in a city in California and has seen a "dramatic reduction in use-of-force and complaints against officers."
The Brown Family is holding a press conference now, tune in to follow the story.
Iowa Supreme Court Questions Constitutionality of 2-Way Video Conferencing in Court
The Sixth Amendment gives us the right to "be confronted with the witnesses against [us]." This is a rule that the U.S. Supreme Court has upheld with few exceptions. The idea is that it is harder for someone to lie if they are facing both the court and the criminal defendant. Therefore, witnesses testifying through video is rarely seen. However, the present question is "whether it would make a difference if a witness testified by two-way video, with witness and accused able to observe each other simultaneously."
The Supreme Court of Iowa ruled last week, saying "it depends." Their reasoning is that while it doesn't fully comply with the constitution, it should be judged on a case-by-case basis using U.S. Supreme Court standards. Justice Edward Mansfield of the Iowa Supreme Court said that "despite its preferability over one-way transmission, [he] does not believe two-way video-conferencing is constitutionally equivalent to the face-to-face confrontations envisioned by the Sixth Amendment."
Source: The Des Moines Register
Expert Witness Fails to Complete Work, Overcharges and then Sues Plaintiffs' Counsel
Ronald Harstad, a professor at the University of Missouri, was retained as an expert witness to calculate damages in a class action lawsuit. The case revolved around "alleged misrepresentations in the sale and marketing of prepaid calling cards." The plaintiffs' counsel (who retained Mr. Harstad) had come to an agreement (via a formal letter) that Mr. Harstad would produce monthly work logs, use students to help at a lower hourly rate, and that he would accept payment for each invoice over time. In April 2008, the plaintiffs' counsel had supplied him with all the necessary information and Mr. Harstad calculated that the total costs for completing his reports would be $17,000 to $21,000.
Irrespective of his estimations, Mr. Harstad was paid $164,604.79 by September. In addition, he failed to complete invoices for October and November 2008 until December when he billed a total of $160,800 for all unpaid services through the end of that year. In January 2009, the plaintiffs' counsel rejected that number and Harstad told them that if they did not pay, they were not allowed to use the reports that he completed. The plaintiffs' counsel decided to hire a new expert witness who was able to complete everything they needed for $22,500.
The plaintiffs' counsel "filed a declaratory judgment action to establish that they owed no futher amounts to Harstad. Harstad counterclaimed for breach of contract and unjust enrichment and sought $410,000 in damages." The court rejected Harstad's claim and decided that no further compensation was necessary. The court explained their decision, noting that Harstad broke the terms of the agreement and he had already received an amount that was far greater than his estimated price. Lastly, the court pointed out that because Harstad chose to void the agreement by refusing to let counsel use his reports, he could not sue for damages based on a breach of contract.
Source: The National Law Review
Check out Mark Torchiana's Interview with Expert Witness Forum
Our Co-founder and CEO, Mark Torchiana, recently spoke with Expert Witness Profiler about Courtroom Insight's innovative knowledge management solution. The interview focused on the history and development of Courtroom Insight. When discussing key benefits and success stories, such as Littler Mendelson, Mark explained how Courtroom Insight differentiates itself from an in-house database.
"Courtroom Insight leverages its public directories of 100,000+ litigation professionals as a starting point. Our customizable templates offer firms an opportunity to begin capturing valuable firm-wide information immediately. We also add significant value by providing additional fully integrated content not available elsewhere. Publicly posted performance reviews, expert witness challenge data and inexpensive custom expert witness research are all available directly from Courtroom Insight. Our value proposition stems from this combination of a ready-made software platform with unique, integrated content."
Click here to read the full interview.
Out of Nearly 6,000 Expert Witnesses to testify in the House, Only 23% were Women
A Sunlight analysis of the 113th Congress reveals that "when it comes to testifying before the House, men get a lot more to say than women". As of September 1, there have been 5,575 witnesses identified to have appeared before House committees and only 23% of those experts have been women. Although women make up a little more than half the U.S. population they are underrepresented in Congress.
There are some committees where women are better represented than others. The House Committee on Agriculture heard from the fewest number of women at 13%, while the Committee on Education and the Workforce heard from the most women ("40 percent of the panel's witnesses were female").
Click on the graph for the breakdown of House witnesses by committee and gender.
Picture Source: Sunlight Foundation


text:
Advice For Specialty Gift Baskets Clarified
Advice For Specialty Gift Baskets Clarified
I would say the main criteria will be getting the photo on your computer digitally. If you only use a print, you 'll need to scan some sort of photo into your computer. If you begin doing not own a scanner, ask a friend for help.
Mother's Day is here! Busy sons or matrimony equality who procrastinated on discovering the perfect present on behalf of mom will breathe one sigh of relief comprehending there's still plenty regarding time to get clever last-minute gift at CVS/pharmacy.
Decide on a theme or color Make your gift container memorable by following the theme of the gardening shop or the parent's preferred color. Sleeping bags, teddy bears, cuddle wraps, picture frames or piggy banks come in an amazing array of shades.
What on earth about the groomsmen? A personalized glass draught beer mug or personalized ale stein makes a great gift idea. Or possibly try an engraved treatment glass. Gifts they'll not only use but yet they probably don't certainly have one like the. A personalized timepiece dinero clip is a traditional gift, but with you see, the added flare of the actual built in watch. Last but not minimal would be a stainless-steel swiss army like bank account knife. Here is more info on specialty gift baskets us take a look at www.youtube.com/watch All blokes like to fix things, so why fight it all.
One of the unique and personalized gifts should give to any little tot is a set of all wooden letters which spell out their name. Majority of these letters can be set up on a wall inside the children's room, on the malbox of the room or even anywhere in house where it is clearly noticed. In fact parents often surprise their children by ordering fancy raw wood alphabet letters which stipulate not just their sites but other things too such as "Congratulations", "Welcome Home" etc.
If you care to make something exclusive for your mom, but you aren't sure you have the ability to pull off a useful gift on your own, the staff at Yucandu Art Studio may manage to help. At Yucandu Art Studio, "you can do" a represent by yourself, using the specific studio's art supplies, a person can work with a coach who will help generate creative ideas and obtain them through until you have a beautiful gift in both your hands.
Dads, before the big day arrives, take your young people to any St. Louis area Michael's crafting or Hobby Lobby organizations. Ask a sales associate to show you to the scrapbooking and young ones crafts section. From there, let your small ones pick out colored paper, stickers, glitter, markers, ribbon or anything that conveys their eye, with the intent of making each paper butterfly craft because mom will love. If your kids would rather make jewelry needed for mom instead, purchase a package of Shrinky Dinks and either some lanyard or a chain from the beads and jewelry articles section of the gather.
As Linux continues to play an ever increasing role in corporate data centers and institutions, ensuring the integrity and protection of these systems must be a priority. With 60% of the world's websites and an increasing share of organization's mission-critical workloads running on Linux, failing to stop malware and other advanced threats on Linux can increasingly impact an organization's reputation and bottom line.
Sponsored by Bit9
Most companies incorporate backup procedures for critical data, which can be restored quickly if a loss occurs. However, fewer companies are prepared for catastrophic system failures, in which they lose all data, the entire operating system, applications, settings, patches and more, reducing their system(s) to "bare metal." After all, before data can be restored to a system, there must be a system to restore it to.
In this one hour webinar, learn how to enhance your existing backup strategies for better disaster recovery preparedness using Storix System Backup Administrator (SBAdmin), a highly flexible bare-metal recovery solution for UNIX and Linux systems.
Sponsored by Storix
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text:
Wilstone House & Gardens - Display Gardens is based in the grounds of an 18th Century Manor House in an area of "Outstanding Natural Beauty" in the heart of the Shropshire Hills.
Here a dedicated design team provide a bespoke project service for both trade and retail clients in Sandstone and Wrought Iron. Each piece is then produced by our skilled master craftsmen using traditional methods.
On display is Wilstone's full hand carved sandstone range, iron gates, water features and an extensive selection of original unique stone and wooden architectural pieces for the garden and home. We have also set aside an acre for the natural ageing of the sandstone pieces for those who prefer an aged look.
Wilstone House & Gardens - Retail Shop is situated on the busy A49 between Ludlow and Shrewsbury and houses a varied selection of eclectic interior and exterior pieces from around the world. Described as a "jewel in the Shropshire crown" by the local press, the shop provides a chance to purchase original items from Europe and India making it a must stop for those searching for unusual stylish pieces.
Come and visit the shop's Facebook page for images and information about our amazing range https://www.facebook.com/wilstonehouseandgardens
Wilstone House & Gardens - Warehouse with over 2,500 sq. ft. of secure storage houses a large supply of ready made pieces for immediate delivery to anywhere in the world to become the antique of the future. Visits by appointment only arranged via the Shop.
Wilstone won the very first RHS Product of the Year award at Chelsea Flower Show with our Charcoal Maker, part of the Kadai Firebowl range. For Kadai Firebowls and accessories, please click here for further information http://www.kadai.co.uk/


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Brian BergerNew Hanover Co. Commissioner Woody White's full statement on BergerSubmitted by WWAY on Fri, 09/06/2013 - 3:24pm.READ MORE:
Inside is a link to White's full statement he made this afternoon during a news conference.
Full story... » FIRST ON 3: Judge restores Brian Berger to New Hanover County Board of CommissionersSubmitted by Holden Kurwicki on Fri, 09/06/2013 - 12:33pm.READ MORE:
N.C. Special Superior Court Judge James Gale has restored Berger to the New Hanover County Board of Commissioners.
Full story... » Berger's attorney thinks judge will rule for ousted commissionerSubmitted by Holden Kurwicki on Fri, 08/30/2013 - 2:43pm.READ MORE:
We are one step closer to getting a ruling in Brian Berger's legal battle with New Hanover County.
Both the county's and Berger's attorney, Chris Anglin, submitted their final briefs this week ahead of today's deadline.
Full story... » Brian Berger accuses New Hanover County of discriminationSubmitted by Brandon Taylor on Mon, 08/19/2013 - 10:22pm.READ MORE:
Former New Hanover County Commissioner Brian Berger has filed an employment discrimination charge against the county.
Full story... » FIRST ON 3: New Hanover Co. files motion to stop Berger's requests for evidenceSubmitted by WWAY on Fri, 08/16/2013 - 2:38pm.READ MORE:
The New Hanover County Commission has requested a protective order against ousted Commissioner Brian Berger as he requests evidence as his appeal of his removal from office continues.
Full story... » New Hanover Co. asks for dismissal in Berger amotion appeal, court costsSubmitted by WWAY on Wed, 08/14/2013 - 2:44pm.READ MORE:
New Hanover County wants Brian Berger's appeal of his removal from office dismissed, and they want him to pay for it.
Full story... » Timeline set for Berger's appealSubmitted by StarNewsOnline.com on Tue, 07/23/2013 - 11:42am.READ MORE:
An official timeline has been set for the rest of former New Hanover County Commissioner Brian Berger's appeal.
Berger was removed from the county board May 20 following an amotion hearing and the ousted commissioner filed an appeal of the decision with the New Hanover County Superior Court in mid-June.
Full story... » Berger amotion appeal heard in GreensboroSubmitted by Holden Kurwicki on Tue, 07/16/2013 - 3:58pm.READ MORE:
It's been nearly two months since New Hanover County Commissioners voted to kick Brian Berger out of office. Today Berger's fight to keep his seat moved to a Greensboro courtroom.
Full story... » Attorney for Berger amotion appeal to cost thousands in taxpayer dollarsSubmitted by Cacky Catlett on Mon, 07/01/2013 - 11:49pm.READ MORE:
While a court is set to hear the appeal of Brian Berger's amotion, the question arises about how much the county will spend on hiring an attorney for the case.
Full story... » New Hanover hires attorney to handle Berger's appealSubmitted by StarNewsOnline.com on Mon, 07/01/2013 - 10:54am.READ MORE:
The price just went up for New Hanover County on former Commissioner Brian Berger's appeal.
The county has hired outside counsel to represent them during the appeal proceedings.
Full story... » |
Recent comments


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For new questions please post at magento.stackexchange.com, the community-run support site for the Magento community. We will be providing updates on the new forum solution soon. For questions or concerns please email email@example.com.
Is there some basic function documentation anywhere? I need to tie magento to a third party system for importing and exporting order information, and automating firing-off of tracking e-mails, etc.
After spending days trying to figure out the basics off how the themes work, which was extremely frustrating and convoluted, largely due to terrible documentation, and now tearing my hair out trying to figure out what some basic functions are called, I'm at my wits end, and ready to switch to another cart.
I mean, maybe I'm missing something here, but I can't seem to quickly find even a basic list of functions or how this thing is built, with out going through code for hours and hours.
Seriously, how are people who've written plugins doing this? I feel like I've looked everywhere and found nothing good at all.


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You won't be able to dismiss this by usual means (escape or click button), but you can close it programatically based on user choices or actions.
"I completely loved my wedding thank you notes i recently ordered from the site. Would definitely refer and reorder of the need comes up!"
"These turned out exactly like I was hoping and better than I was expecting. At first I thought they were a little pricey but not having to stuff them in envelopes and with the stamp discount I figured it would be worth it. And when they arrived and looked so dang adorable, I realized it was definitely worth the money. It's a very good quality, thick card that has just enough space to write a brief message on the back. The picture quality was fantastic and it was very easy to upload and personalize the card on the website. I've had tons of complements and people keep asking me who did them. I've recommended wedding paper divas to all the brides and bride-to-be's that I know! No regrets!!"
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"I was going for something very sweet, classic and simply beautiful. This card lived up to my requirements and so happy when I received it. I chose the gold foil on blush colored paper with my name at the bottom. I kept the back simple with no pattern/photo/text on it and the corners were cut out. Even with these minimal designs, the card is absolutely lovely."


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Derby County Football Club
- Nickname:The Rams
- Founded:1884
- Manager:Steve McClaren
- Ground:Pride Park
- Capacity:33597
- Last Game: 24-Feb-2015 19:45
- Derby County2
Charlton Athletic0 - Full match report
Davies delight at final berth
16 May 2007 - 10:29:10
Derby County boss Billy Davies felt justice was done after the Rams sealed a place in the Championship play-off final following a penalty shoot-out victory over Southampton.
Darren Moore had extended Derby's one-goal first-leg advantage with a third minute header, but Jhon Viafara and Grzegorz Rasiak netted either side of a Leon Best own goal to send the match into extra-time.
In the shoot-out, Derby held their nerve to secure a Final berth following misses by Best and former County midfielder Inigo Idiakez.
"It was a great game of football, real end-to-end stuff," admitted Davies.
"After we'd scored so early I thought we just needed to keep Southampton quiet for the next five minutes and we'd be on our way, only for us to give away a stupid goal in typical Derby County fashion.
"Then they get another one, we pull one back and then Rasiak, a former player here, scores late on to force extra-time.
"At the end of the regulation 90 minutes I told the players that over two legs we'd done enough to deserve to go to Wembley and that they must believe it would be their night, that justice would be done.
"We were the only team who looked like winning it in extra-time but in the end we needed four well-taken penalties."
Related Derby County News
Related Derby County News


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Diamond Heart Love Necklace in 14 Karat White Gold
He had heard the word love so many times, maybe even said it himself, but he never knew what it really meant until he met her. She made him the happiest man in the world, and he wants to show her the depth of his devotion. More
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Mr. and Ms. Little Head Start 2014
The Tunica Head Start Center sponsored a King and Queen Drive/Sweetheart Ball in February. Officials thank parents for participating in this event and all events at the Center.
Latest News
County claims as of Feb. 2, 2015 The claims docket approved...
Board cuts monthly expenses by $120,000 per month County administrator...
A Whole New World Tunica Academy students...
Fire departments, co. utility in cash crunch Budget and cash flow...
Old Man Winter takes residence in Tunica Area schools took a snow...
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Text messaging while driving is wrong regardless of driver's age
Text messaging isn't the only problem. Talking on the phone, applying makeup and drinking coffee take a driver's attention away from where it should be: the road.
Today's Question: Finding opportunity in a down economy
More people than ever are applying for graduate schools in the face of a tough economy. But with more people earning advanced degrees, the job market is becoming flooded with "overqualified" workers. Is the deflated economy a good opportunity to pursue a advanced degree?
Media should be careful with 911 calls
The media needs to be thoughtful about when and how emergency recordings are used. Using them for scream-value alone is to risk a valuable means of protecting public safety and endanger the privacy of victims for something that often looks like an inane attempt to turn the news into "24."
GUEST COLUMN: The valor of patriots remembered on Memorial Day
The holiday was first begun to honor those who died in the Civil War. Today, we celebrate the brave soldiers who protect the peace at home and overseas.
GUEST COLUMN: What makes a great city?
Are the characterstics and qualities of a great city different for ethnic minority residents?
Guests often become friends for Pepper and Mouser
Many of the people who have appeared on "Pepper and Friends" over the past 27 years have been more then interview subjects. And they have been a variety of characters. "We run the gamut, I think, in local community television," co-host James Mouser said.
The Dennis Skillicorn execution: Why it was front page news
State-sanctioned executions are the government's ultimate expression of power, and therefore newspapers bear the responsibility of shining a light on the event.
CARTOON: The Magnificent Seven
Leonard Griswell's take on geographic policing in downtown Columbia.
Eurovision Song Contest: the world's version of American Idol
Internationally, this competition attracts an audience of over 100 million from more than 50 different countries.
Today's Question: A new plan for downtown businesses
The Special Business District and the Central Columbia Association are looking to merge into one entity — a community improvement district. Is a community improvement district a good idea for downtown? Why or why not?
GUEST COLUMN: Changing helmet law makes no sense
Changing the law to allow motorcycle riders over the age of 21 to ride without a helmet is a step backward.
The report of newspaper industry's death is greatly exaggerated
Most individual newspapers are making money, and readership is higher than ever.
Today's Question: Putting restrictions on credit for young people
Congress has passed a bill that would tighten credit for those younger than 21 — if they don't have a co-signer or can show a way to pay it back, they won't get a credit card. Will age restrictions for obtaining credit cards solve credit problems for young people?
Columbia offers no bailouts for Dodge City as Chrysler exits
With Chrysler telling Dodge City Motors to get out of town, the question emerges — who will stand up for small businesses in Columbia? The more I learn, the more it seems as though the answer is: No one.
GUEST COLUMN: Thirty years later, Aslanidis family at G&D still as wonderful as ever
The kindness shown to a young, financially struggling couple by the owners of G&D Steakhouse is still alive after more than 30 years.
LETTER: Blagojevich case shows there will always be corruption
While the former governer has received most of the media attention, little attention has been paid to those aides who worked for him, many of whom continue to serve in state government.
iPhone mandate from MU School of Journalism laced with problems
Experience as an MU student shows that the School of Journalism's iPhone/iPod Touch requirement is probably unnecessary, which leads to more questions about the decision to make the devices mandatory.
Today's Question: iPhone competition raises questions over ownership
Four MU students developed an award-winning application as part of a university-sponsored competition. Should the students have 100 percent of the rights to the application they developed using university property?
If gardening space is limited, be creative with containers
Mix and match to create gardens that are fun and compact.
Today's Question: Should Missouri adopt a consumption tax?
The idea of a "fair tax" — one based on what people spend, not what they earn — is gaining in popularity. What are the advantages or disadvantages of a consumption tax?


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Author: crossley
Date: Sat Jun 12 22:57:57 2004
New Revision: 21155
Refine the "committers" section after discussion on forrest-dev
Modified: forrest/trunk/src/documentation/content/xdocs/guidelines.xml
--- forrest/trunk/src/documentation/content/xdocs/guidelines.xml (original)
+++ forrest/trunk/src/documentation/content/xdocs/guidelines.xml Sat Jun 12 22:57:57 2004
@@ -74,7 +74,7 @@
When developers remain with the project and demonstrate commitment
to the project in various ways, then they will be noticed and
@@ -86,14 +86,6 @@
- they are given more responsibility and can do more work.
- The responsibilities of committers include:
All Apache committers are required to have a signed Contributor
License Agreement (CLA) recorded on file with the Apache Software
Foundation. The
@@ -106,14 +98,17 @@
From a project management perspective, and as defined by the
- ASF bylaws,
- the project committers form the PMC. The committee is responsible
+ ASF bylaws,
+ the project committers form the Project Management Committee.
+ The PMC is responsible
to the Board and the ASF for the management and oversight of the
Apache Forrest codebase and community.
(more info)
The PMC responsibilities include: The Project Management Committer responsibilities include:


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Antonio Sabato Jr. Says, "I Do." Luke Kerr September 26, 2012 General Hospital, My Antonio, TV 8 Comments Former General Hospital star Antonio Sabato Jr. has tied the knot with Cheryl Moana Marie, according to People. Reportedly, the couple married at a private estate in Hawaii. Congratulations to the Sabatos! Photo by PR Photos
Antonio Sabato Jr. and Cheryl Moana Marie Expecting Son Luke Kerr January 20, 2011 General Hospital, General Hospital: Night Shift, My Antonio, SOAPnet, VH1 Former General Hospital and General Hospital: Night Shift star Antonio Sabato Jr. and girlfriend Cheryl Moana Marie are expecting a baby boy, according to People. Reportedly, the couple plans to name their son Antonio Sabato III. Congrats to the couple! Photo by PR Photos
Antonio Sabato Jr. Brings Daughter to Million Milkshakes Opening Luke Kerr October 24, 2009 General Hospital, General Hospital: Night Shift, My Antonio 3 Comments My Antonio and former General Hospital: Night Shift star Antonio Sabato, Jr. brought daughter Mina Sabato and friends Amanda and Kylie and to the launch of Millions of Milkshakes, his new store in West Hollywood. See more pictures and watch video after the jump.
Antonio Sabato Jr. Searches for Love on My Antonio Luke Kerr July 15, 2009 General Hospital, General Hospital: Night Shift, My Antonio, News, Reality 8 Comments Antonio Sabato Jr. is looking for love and his quest to find hat "special someone" will unfold on My Antonio, a VH1 show premiering August 16th. The 10-episode series begins with Antonio's sexy swim ashore to meet the 13 beauties vying for his attention. Within the first few moments, Antonio makes a shocking elimination, leading the women to realize that anything can happen when Antonio is in charge. "I've been with some of the most amazing women in the world," says Sabato, "and at the age of 36, I'm finally ready to settle down. I really hope to meet that special someone. For me this is not a game. This is real. This is my life."


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Free Mental Health First Aid Classes Available to Public
POSTED: Tuesday, May 8, 2012 - 11:59am
UPDATED: Tuesday, May 8, 2012 - 12:09pm
EL PASO — Doña Ana County will offer certified Mental Health First Aid Training to help the public identify, understand, and respond to signs of mental illness and substance abuse disorders. This groundbreaking public education program is offered at no cost to the public.
The interactive 12-hour course is an overview of mental illnesses and substance-abuse disorders that gives participants an opportunity to learn how to recognize risk factors and warning signs of mental health problems, understanding their impacts and identifying common treatments.
Anyone who takes the free 12-hour course will be certified as a Mental Health First Aider and will learn a 5-step action plan that includes skills, resources and knowledge to help those in crisis connect with the appropriate care.
Mental Health First Aid Training is offered at the following times and locations:
Doña Ana County Government Center
845 N. Motel Blvd., Las Cruces, N.M.
May 10 and 11, 9 a.m. to 4 p.m.
Vado/Del Cerro Community Resource Center
180 Le Fe Avenue, Vado, N.M.
May 14 and 15, 9 a.m. to 4 p.m.
For more information or to register in one of the above trainings, please contact Sabrina Tang at (303) 541-0311 or by email at email@example.com. The registration form is also available on the Doña Ana County web site at http://www.donaanacounty.org/health/.


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Gunns may have traded insolvent: report
Market watch top headlines
Australian reports
- Aust markets: Resources sector weighs on share market
- Aust dollar report: $A weakens ahead of US non-farm payrolls
- Aust credit close: Bond market flat ahead of US jobs data
World reports
- World commodities: Commodities markets summary
- World markets: International markets roundup
Timber giant Gunns may have traded while insolvent, administrators of the collapsed company say.
In a detailed report to creditors, PPB Advisory says more investigation is needed to determine the date of insolvency.
But the report says Gunns may have had solvency concerns six months before it entered administration on September 25 last year.
"As we are yet to form a conclusive view on the date of insolvency we are unable to state definitively whether the Gunns Group traded whilst insolvent," the report said.
"Additional investigations will be required by a liquidator (if appointed) before a view may be formed."
The administrators' "preliminary view" is that Gunns was insolvent from "at least" September 21.
But the report says solvency concerns could have existed when prospective partner in the company's proposed northern Tasmanian pulp mill, the Chandler Corporation, pulled out in March, 2012.
PPB says there were also concerns in July, when Gunns announced it was relying on the support of lenders, and September 12 when lenders knocked back a request for funding.
It says it is continuing to investigate the possible use of third party funds from managed investment schemes among a number of potential breaches by directors.
The report concludes Gunns has debts of around $3 billion and recommends the company be liquidated.
It says lenders owed $446 million are unlikely to be paid in full and unsecured creditors owed $2.4 billion will not see any return.
Workers' entitlements totally $10 million will be met, the report says, but the timing of payments remains uncertain.
A second meeting of creditors in Launceston next Tuesday will determine whether the company should be wound up.
David Beniuk


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Breaking News
Women's Big Ten tournament: Minnesota advances to play Ohio State
Minnesota 82, Purdue 78: Shae Kelley scored 26 points, Amanda Zahui B. added 21 points and 11 rebounds and Minnesota defeated Purdue in the women's Big Ten tournament in Hoffman Estates, Ill., last night.
Read More
Women's basketball: Buckeyes seek to keep focus in postseason
A year ago, Ohio State entered the Big Ten tournament as an eighth seed with the postseason life expectancy of a goldfish dropped into a tankful of largemouth bass.
Read More
Bob Hunter commentary | Rumblings: Ohio State football recruiting is off to quick start
Defensive end Terrell Hall's commitment to Ohio State this week affirmed that Urban Meyer and his coaches are off to a hot start in filling their 2016 class.
Read More
The Latest From
The Locker Room
The Extremely Rare Healthy Blue Jacket
It only took a couple of games. David Clarkson succumbed to the curse almost immediately after being traded to Columbus; and now he's out, most likely for the season, with a torn oblique.
Yes, that would be the same David Clarkson whom the CBJ obtained because Nathan Horton was hurt.
Injuries have so decimated the Blue Jackets this season that it seems a healthy CBJ skater is incredibly rare. Dare we say endangered?
For this reason, we actually contacted the Department of Fish and Wildlife, who gave us the following response:
The healthy Blue Jacket "cannot be listed under the Endangered Species Act because they do not qualify as a distinct species or subspecies."
So there you have it.
No matter how many man-games the Jackets lose (currently 406…and counting), they cannot get federal protection from a Cal Clutterbuck hit or a Jordin Tootoo high stick.
The very nice folks at the Department of Fish and Wildlife did wish the Blue Jackets the best of luck. Heaven knows they need it.
Read More
College wrestling: Big Ten shows it is big-time
IOWA CITY PRESS-CITIZEN PHOTODan Gable coached Iowa to 15 NCAA titles and 21 consecutive Big Ten championships from 1976 to '97.
The iconic Dan Gable will hobble through St. John Arena this weekend with bad knees and artificial hips, but difficulty getting around won't dampen his spirit. Already, the thought of the Big Ten wrestling championships on Saturday and Sunday at Ohio State has the sport's mythical warrior rife with anticipation.
Read More
NHL: Blue Jackets might be facing glut at forward
The Blue Jackets' short-term view is clear: They hope to end a seven-game losing streak tonight against the New Jersey Devils at the Prudential Center.
Read More
Blue Jackets notebook: Dubinsky back after 'scary' injury
EAMON QUEENEY | DISPATCHBrandon Dubinsky hits the ice after being undercut by Montreal's Tom Gilbert (77) on Feb. 26.
Brandon Dubinsky's last goal was beautiful. His last goal celebration? Let's just say it was cut short.
Read More
Soccer: Crew welcomes pressure to succeed
Barbara J. Perenic | DISPATCHThe Crew's Tony Tchani, center, said the team must stay focused despite higher expectations this season.
Tony Tchani is a private person by nature. Physically imposing on the soccer field, he can be soft-spoken and reserved away from it when it comes to personal matters. Goals, hopes and expectations are kept contained within, leaving others to speculate and assess.
Read More
Soccer | Crew preview: 5 questions for MLS
ASSOCIATED PRESS FILE PHOTOForward Jozy Altidore, a member of the U.S. national team, returns to MLS to play for Toronto FC.
As part of the addition of expansion clubs Orlando City SC and New York City FC and the dissolution of Chivas USA, the balance of power has shifted a little more to the west.
Read More
Around baseball: Indians' Bourn feels faster after track work
Michael Bourn got back in the starting blocks this winter. A track sprinter in high school, Cleveland's speedy outfielder had some hurdles to clear.
Read More
Big Ten wrestling: Buckeyes fully healthy at just the right time
Ohio State wrestling coach Tom Ryan finished a talk with his team before practice last week by asking if anyone had anything to say. Logan Stieber, a fifth-year senior co-captain and a three-time national champion, stood up.
Read More
Around college basketball: Kentucky starts slow, improves to 28-0
Nam Y. Huh | Associated PressIndiana coach Tom Crean reacts to a call during the Hoosiers' loss at Northwestern.
No. 1 Kentucky 74, Mississippi State 56: Kentucky fought off a slow start but still cruised to a Southeastern Conference victory over host Mississippi State last night to keep its undefeated season on track.
Read More
Women's basketball: Ohio State playing for double bye in Big Ten tournament
The Ohio State women's basketball team has reached the final two games of the regular season with the chance to accomplish something that few thought possible in November: earning a double bye for the Big Ten tournament.
Read More
Sports roundup: County approves $2 billion Los Angeles stadium plan
The Inglewood (Calif.) City Council unanimously approved a $2 billion stadium plan late Tuesday backed by St. Louis Rams owner Stan Kroenke that clears a path for the NFL to return to the Los Angeles area for the first time in two decades.
Read More
Soccer: Winter World Cup plan angers critics
FIFA's scheduling committee formally recommended yesterday what most had been expecting for the 2022 World Cup in Qatar: moving the soccer tournament from the summer to the cooler months, between Thanksgiving and Christmas.


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Milton Center Historic District
Milton Center Historic District
Milton Congregational Church
Location | Roughly bounded by Milton, Shearshop, Headquarters, Sawmill, and Blue Swamp Rds., Milton, Connecticut |
Coordinates | 41°46′3″N 73°16′22″W / 41.76750°N 73.27278°WCoordinates: 41°46′3″N 73°16′22″W / 41.76750°N 73.27278°W |
Area | 180 acres (73 ha) |
Built | 1750 |
Architect | Dickinson,Oliver; Et al. |
Architectural style | Greek Revival |
Governing body | Local |
NRHP Reference # | 86003754[1] |
Added to NRHP | December 23, 1986 |
The Milton Center Historic District is a 180 acres (73 ha) historic district in the town of Litchfield, Connecticut, that was listed on the National Register of Historic Places (NRHP) in 1986.[1] It includes 20 buildings deemed contributing to the historic character of the district, one additional contributing structure, and one contributing site.
Notable buildings in the district include the David Welch House and the Trinity Church, which are both separately NRHP-listed. The district includes the Milton Academy and a Milton District School. It includes the Milton Congregational Church, built in 1791. The district includes a common, the Blue Swamp Burial Ground (a Revolutionary War era cemetery), and two bridges over the East branch of the Shepaug River. The district is embedded in a larger district which is locally designated and encompasses 150 buildings.[2]
See also[edit]
- ^ a b "National Register Information System". National Register of Historic Places. National Park Service. 2009-03-13.
- ^ David F. Ransom and John Herzan (February 9, 1986). "National Register of Historic Places Inventory-Nomination: Milton Center Historic District". National Park Service. and Accompanying 19 photos from 1986 and one from 1926
This article about a property in Connecticut on the National Register of Historic Places is a stub. You can help Wikipedia by expanding it. |


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Regulator to keep charities honest
PUBLISHED: 16 Jan 2012 00:01:34 | UPDATED: 16 Jan 2012 05:36:46PUBLISHED: 16 Jan 2012 PRINT EDITION: 16 Jan 2012The head of the country's newest regulator is an optimist, which is a good thing because Susan Pascoe has just six months and $53 million to set up the first federal overseer of the $43 billion non-profit industry.
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Sorry this item is currently sold out. Please use this discount coupon for an additional 5% off during checkout:
The Hadley is back again this year to keep you warm while giving you a really soft feel against your skin. This is a must have whether you are cruising down the mountain or sending it in the park.