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Moving Cancer Stem Cells toward the Clinic
The science of cancer stem cells and how the cells might be used to prevent and detect cancer was the focus of a workshop last week on the NIH campus. The meeting was sponsored by NCI's Division of Cancer Prevention (DCP).
Several hundred researchers heard presentations on what is known - and not known - about the molecular biology of cancer stem cells. Experts discussed genetic and epigenetic alterations, signaling pathways, and influence of the tumor microenvironment on cancer stem cells. Read more
Guest Update by Dr. Henry Rodriguez
CPTI Will Help Realize the Promise of Proteomics
There are many in the cancer research community who believe proteomics has tremendous potential, particularly for the early detection of disease. Unfortunately, current proteomic methodologies have not sufficiently addressed the lack of reproducibility of measurements from run to run, instrument to instrument, or laboratory to laboratory. This lack of standards and reproducibility led NCI to develop and launch the Clinical Proteomic Technologies Initiative for Cancer (CPTI) last September. The major goals of CPTI are to optimize current proteomic technologies and develop the new technologies, reagents, systems, and working teams needed to realize proteomics' promise.
Following several years of input from the international proteomics and cancer communities, CPTI was designed to provide a highly organized approach to assess, refine, develop, and apply proteomic technologies and data resources to support the discovery of biomarkers for all aspects of cancer research, especially early detection of cancer and to monitor therapeutic outcomes. This model will also lead to effective ways of addressing the barriers that exist early in the biomarker discovery pipeline. Read more
Moving Cancer Stem Cells toward the Clinic
The science of cancer stem cells and how the cells might be used to prevent and detect cancer was the focus of a workshop last week on the NIH campus. The meeting was sponsored by NCI's Division of Cancer Prevention (DCP).
Several hundred researchers heard presentations on what is known - and not known - about the molecular biology of cancer stem cells. Experts discussed genetic and epigenetic alterations, signaling pathways, and influence of the tumor microenvironment on cancer stem cells.
Many researchers were enthusiastic about one day using cancer stem cells in the clinic. But they acknowledged that a lack of cancer stem cells (and patient samples) is preventing the field from exploring basic questions, such as the origins of these cells.
"The meeting reinforced the view that we need to develop technologies to isolate and characterize cancer stem cells," said Dr. Sudhir Srivastava of DCP. "Time and again people talked about having cell lines and culture systems so that more laboratories can study these cells."
Recent studies have supported a role for cancer stem cells in early detection, noted Dr. Srivastava, who heads the Cancer Biomarkers Research Group. But the studies have not been replicated because of a lack of adequate cancer stem cell samples from patients.
Cancer stem cells are hard to isolate because they are so rare - perhaps as few as one tumor cell in a million. The first such cells were discovered in patients with leukemia in 1997. More recently, the cells have been reported in breast, colon, and brain tumors.
Like the stem cells that repopulate adult tissues, cancer stem cells are thought to perpetuate themselves while also giving rise to diverse types of tumor cells.
In a keynote address, Dr. Michael Clarke of Stanford University said that it will be important to have diagnostic tests that can identify precancerous lesions. By the time cancer stem cells develop it is often too late to help the patient, he noted.
Dr. Clarke also stressed the critical need to understand the mechanisms by which cells escape the normal constraints on self-proliferation.
Another keynote speaker, Dr. Stewart Sell of the Ordway Research Institute, discussed how the tumor microenvironment may influence the behavior of cancer stem cells. The surrounding environment may be as important as the intrinsic properties of the cancer stem cell, he said.
He and others discussed the need to characterize genetic pathways that are deregulated in cancer stem cells, particularly those involved in self-renewal. This could lead to strategies for identifying and blocking key signals in mutant stem cells.
Cancer stem cells could also be targets for drugs to prevent cancer in patients at risk of the disease, including those with premalignant lesions, commented Dr. James Crowell, who heads NCI's Chemopreventive Agents Development Research Group.
The need for cancer stem cell markers was a recurrent theme. Many researchers said that multiple markers may be required to capture the genetic diversity of cancers.
Building on the success of the workshop, DCP is consulting with experts on how best to support basic research on cancer stem cells that can be translated into clinical tools.
Drs. Jacob Kagan and Levy Kopelovich of DCP, who organized the workshop, are writing a summary of the meeting and recommendations for publication.
— Edward R. Winstead
Guest Update by Dr. Henry Rodriguez
CPTI Will Help Realize the Promise of Proteomics
There are many in the cancer research community who believe proteomics has tremendous potential, particularly for the early detection of disease. Unfortunately, current proteomic methodologies have not sufficiently addressed the lack of reproducibility of measurements from run to run, instrument to instrument, or laboratory to laboratory. This lack of standards and reproducibility led NCI to develop and launch the Clinical Proteomic Technologies Initiative for Cancer (CPTI) last September. The major goals of CPTI are to optimize current proteomic technologies and develop the new technologies, reagents, systems, and working teams needed to realize proteomics' promise.
Following several years of input from the international proteomics and cancer communities, CPTI was designed to provide a highly organized approach to assess, refine, develop, and apply proteomic technologies and data resources to support the discovery of biomarkers for all aspects of cancer research, especially early detection of cancer and to monitor therapeutic outcomes. This model will also lead to effective ways of addressing the barriers that exist early in the biomarker discovery pipeline.
Through interactions with NCI and federal and private sector groups, CPTI is expected to catalyze targeted discovery and development efforts. The purpose of these partnerships is to overcome the obstacles to recent attempts at applying protein measurement technologies - namely mass spectrometry (MS) and affinity-based detection methods - to clinical applications.
CPTI is addressing these hurdles through its three integrated programs. The team-based component is the Clinical Proteomic Technology Assessment for Cancer (CPTAC), consisting of five networked technology assessment centers that conduct rigorous technology assessment, develop standard protocols and clinical reference sets, and evaluate methods to ensure data reproducibility. Centers include the Broad Institute of MIT and Harvard, Memorial Sloan-Kettering Cancer Center, Purdue University, the University of California, San Francisco, and Vanderbilt University School of Medicine. Performed in conjunction with partners such as the National Institute of Standards and Technology, CPTI has assembled a multidisciplinary team that represents a broad range of commercially available MS proteomic platforms and analysis software to provide the most comprehensive approach to assess intraplatform and interlaboratory variability to the measurement of proteins in clinical specimens.
The other components of CPTI are the principal investigator-driven Advanced Proteomic Platforms and Computational Sciences and the Proteomic Reagents Resource. The former involves the development of innovative affinity-based and MS technologies, including software algorithms that support quantitative analysis of peptides and proteins, while the latter serves as a public resource for high-quality, well-characterized proteomic reagents (proteins, peptides, antibodies, proficiency testing materials) and other resources. Recently, CPTI's experience and the Reagents Resource component served as a model for and played a leading role in the 2008 NIH Protein Capture/Proteome Tools Roadmap Initiative.
Through its extramural community, CPTI aims to support the needed foundation for proteomics working in partnership with existing NCI resources, including caBIG, the Early Detection Research Network, and the SPOREs, to create a national proteomics research infrastructure. CPTI also integrates with NCI's technology programs such as the Innovative Molecular Analysis Technologies program, Office of Cancer Genomics, Office of Biorepositories and Biospecimen Research, the Small Business Innovation Research program, and the NCI Alliance for Nanotechnology in Cancer, among others.
It's truly rewarding to be part of this effort, one that I believe can create a paradigm shift in biomarker discovery and have a significant impact on cancer detection, diagnosis, and treatment.
Common Genetic Variants Linked to Breast Cancer
Researchers have identified common genetic variations associated with breast cancer in several populations of women. The variants occur in a tumor suppressor gene called FGFR2 (Fibroblast Growth Factor Receptor 2), which was previously reported to be amplified or overexpressed in some breast cancers.
Two independent research teams - one in the U.S. and one in the U.K. - made the discovery by conducting genome-wide association studies to look for breast cancer susceptibility genes. They scanned DNA from thousands of women with breast cancer and from healthy women. In both studies, variants in FGFR2 were linked to disease risk.
"This gene is a substantial new risk factor for breast cancer," says Dr. David Hunter of the Harvard School of Public Health and NCI, and lead author of the U.S. study. "The discovery opens up new avenues for research on the gene and its signaling pathway, which may have relevance to treatment and prevention."
For women of European descent, a single copy of the variant gene increases breast cancer risk by 20 percent while two copies increase risk by 60 percent, or about the same amount as having a single family member with the disease. Approximately 15 percent of white women are estimated to carry two copies of the variant gene.
Dr. Hunter says that it would be premature to test women for the FGFR2 risk variants. Additional variants are likely to be found as the researchers analyze the genome data generated as part of the study, conducted under NCI's Cancer Genetic Markers of Susceptibility (CGEMS) program.
In the study's next phase, CGEMS researchers are evaluating 29,000 variants linked to breast cancer risk during a series of collaborative studies, mostly drawn from the NCI Breast & Prostate Cancer Cohort Consortium. The aim is to build a catalogue of genetic risk factors that can be evaluated along with environmental factors, such as hormone use.
"As we sift through the genome-wide association studies, we are almost certain to find more hits that will have important implications for our understanding of breast cancer genetics," says Dr. Hunter, who is an NCI Eminent Scholar.
The findings appeared online in Nature Genetics on May 27. The same day, the U.K. group, which included researchers from NCI's Division of Cancer Epidemiology and Genetics (DCEG), published findings online in Nature. Dr. Douglas Easton of Cancer Research UK at the University of Cambridge and his colleagues identified FGFR2 and three other novel breast cancer susceptibility genes (TNRC9, MAP3K1, and LSP1).
The function of these genes in breast cancer susceptibility is not known. Taken together, the new findings provide leads for identifying new biological processes in breast cancer and may contribute to the familial risk not explained by BRCA mutations.
"It's exciting and also very reassuring to see that we quickly identified a gene that was also found by another group," says Dr. Stephen Chanock, director of the NCI Core Genotyping Facility in DCEG and senior author of the CGEMS study.
Dasatinib Effective Against Difficult-to-Treat ALL
The results of a 36-patient phase II clinical trial indicate that the multitargeted drug dasatinib (Sprycel) may be extremely beneficial in adult patients with a form of acute lymphoblastic leukemia (ALL) who have developed resistance or do not respond to another targeted agent, imatinib (Gleevec). The drug's effectiveness did not appear to be hampered by most of the mutations in a key protein that have been associated with imatinib resistance.
Patients in the trial had a specific chromosomal translocation, often referred to as the Philadelphia chromosome, that is associated with a rapid course of disease after ALL diagnosis and poor survival. The translocation creates a fused protein known as BCR-ABL, the same protein typically seen in chronic myelogenous leukemia, for which imatinib has proven to be an effective treatment.
"These data are highly significant given the refractory nature of patients enrolled in this trial to current treatment modalities, including imatinib," wrote study leader Dr. Olivier Ottmann from Johann Wolfgang Goethe University in Germany and colleagues in a May 11 early online release in Blood.
In the study, patients given dasatinib at 70 mg twice daily had strong hematologic and cytogenetic response rates - meaning a return of normal white blood cell counts and a significantly reduced number of cells positive for the Philadelphia chromosome, respectively. At 8 months, 42 percent of patients had a major hematologic response, of whom two-thirds exhibited no disease progression; 58 percent of patients had complete cytogenetic responses.
Cisplatin Improves Survival for Women with Cervical Cancer
Long-term follow-up results from a Gynecologic Oncology Group clinical trial that compared cisplatin-based chemotherapy with hydroxyurea in addition to radiation therapy for locally or regionally advanced cervical cancer, published online in the Journal of Clinical Oncology, showed that cisplatin-based chemotherapy significantly improved both progression-free and overall survival compared with hydroxyurea alone. The percentage of women experiencing late side effects did not significantly differ between the treatment groups.
The investigators randomly assigned participating women to one of three groups. One group received cisplatin alone, the second received the chemotherapy drug hydroxyurea alone, and the third received a combination of cisplatin, hydroxyurea, and the drug 5-fluorouracil (5-FU). All drugs were given during radiation therapy, and all women received the same type and amount of radiation therapy.
Patients were followed for an average of almost 9 years. Women who received either cisplatin or the combination of cisplatin, 5-FU, and hydroxyurea had significantly longer progression-free survival and overall survival than women who received hydroxyurea alone, regardless of whether the cancer had spread locally or regionally. After adjusting for the fact that more patients who received cisplatin or the combination regimen were alive for the analysis of side effects, the investigators did not observe a significant difference in late-occurring side effects between the groups.
"Collectively, this follow-up analysis continues to support the use of cisplatin-based concurrent chemotherapy with pelvic radiation therapy for locally advanced stage cervical cancer," summarized the authors.
Caspase-8 Drives TRAIL Resistance in Ewing's Sarcoma
Scientists at the Albert Ludwigs University of Freiburg in Germany and their collaborators at NCI's Center for Cancer Research (CCR) have identified both a potential mechanism for resistance of Ewing's sarcoma (ES) to an experimental therapeutic protein called tumor necrosis factor apoptosis-inducing ligand (TRAIL), and a possible method for overcoming this resistance. Their results were published in the June issue of the American Journal of Pathology.
The investigators focused on caspase-8 expression because lack of the protein has been linked in laboratory studies to TRAIL resistance in ES cells and other tumors. They first looked at tissue samples taken from 47 patients with ES. In three-quarters of the samples, caspase-8 expression was detected in 60 to 100 percent of the cells. In the rest of the samples, caspase-8 expression was detected in only 0 to 50 percent of the cells. Therefore, within any individual ES tumor, cells that lack caspase-8 could cause resistance to TRAIL.
The investigators next tested whether interferon-gamma (IFN-γ), which has been shown in the laboratory to increase caspase-8 expression in cells, could sensitize ES cells with low expression of caspase-8 to treatment with TRAIL. They found that doses of IFN-γ within the range easily tolerated by patients increased caspase-8 expression in caspase-8-deficient cell lines. When the IFN-γ-treated cells were treated with TRAIL, they underwent apoptosis, indicating restored sensitivity to TRAIL.
Additional in vitro experiments showed that chemotherapy does not select for tumor cells that lack caspase-8, which would make subsequent treatment with TRAIL less effective. Changing the levels of caspase-8 expressed by the cells, either genetically or by adding IFN-γ, did not alter their sensitivity to chemotherapy, indicating "that the combination of TRAIL and IFN-γ with standard chemotherapeutics in ES could be feasible," stated the authors.
WTX Found to be a Tumor Suppressor in Wilms' Tumor
A new study has found that a protein called WTX is an important factor in the protein-destruction complex targeting β-catenin when Wnt signaling is misguided, according to results published online May 18 in Science. β-catenin activation causes Wilms' tumor.
Dr. Michael B. Major of the Howard Hughes Medical Institute and colleagues initially used proteomic methods to determine which proteins bind to β-catenin in cell lysates. They found that WTX interacts with β-catenin in the protein-destruction complex. To further investigate WTX activity, researchers generated cultured cells that express WTX. They also manipulated xenopus and zebrafish embryos to examine the effects of the Wnt gene with and without WTX.
Looking at the generated cultured cells, researchers found that WTX partners with other destruction-complex proteins to promote degradation of β-catenin. They also found that the xenopus embryos injected with the Wnt gene developed two heads, while those injected with Wnt and WTX developed milder head anomalies. They saw similar results in the zebrafish embryos. The researchers concluded that WTX negatively regulates Wnt/β-catenin signaling. Additionally, based on the ability of WTX to regulate β-catenin, it is a tumor suppressor gene in Wilms' tumor.
In an editorial, Dr. Roel Nusse of Stanford University School of Medicine noted that "β-catenin activity has been detected in other human cancers, mostly by virtue of the nuclear presence of the β-catenin protein. In many of those cases, there has been no evidence that the known components of the WNT signaling pathway are mutated, suggesting that β-catenin becomes activated without any genetic alterations. But the new knowledge by Major et al. invites speculation that WTX is in fact mutated in these cancers."
Lymphedema After Cancer - How Serious Is It?
Many people who survive cancer will suffer from a serious side effect of treatment known as lymphedema, and they may not even know about it.
The lymphatic system comprises a very fine network of vessels and filtering nodes that circulate lymph (the fluid that bathes cells with nutrients and helps clear away waste and infection) throughout the body. Lymphedema results when part of this system is interrupted - when lymph nodes or vessels are removed or damaged, causing a "traffic jam" of lymph to build up.
Patients often describe the resulting swelling in the distal limb or tissue on the side of the body where the damage occurred as feelings of heaviness, fatigue, and tightness. Over time, swelling can increase dramatically and be accompanied by sclerosis that makes it permanent.
Recent research supports that contention. In the largest prospective study of its kind, published last month in Cancer Epidemiology, Biomarkers & Prevention, 32 percent of women aged 45 years and younger treated for breast cancer had persistent swelling in the hand and/or arm 3 years after surgery. The incidence of episodic swelling was 54 percent.
"This doesn't just happen in a few women," says the study's lead investigator, Dr. Electra Paskett from Ohio State University's College of Public Health and Comprehensive Cancer Center. "It's a serious consequence of treatment in terms of magnitude." Dr. Paskett is also a breast cancer survivor who lives with lymphedema.
"When I was diagnosed with breast cancer 10 years ago, during radiation I developed swelling in my affected hand and index finger," she continues. "My radiation oncologist referred me to a physical therapist, and that's how I found out about lymphedema."
Dr. Paskett's subsequent research with breast cancer survivors showed that lymphedema can have a devastating impact on quality of life, and that communication between physicians and patients on the risks for and signs of lymphedema was sparse, when it happened at all.
Why one patient may develop lymphedema while another does not is still unclear. In general, the greater the number of lymph nodes removed, the higher the risk. The risk after axillary node dissection is much greater than after sentinel node biopsy. Radiation is another risk factor, as is obesity and infection after surgery.
Everyone agrees that there is a great need for patients and physicians to understand risks for and symptoms of lymphedema, and know that there are ways to treat it and manage it.
"It's about creating pathways for the lymph fluid to drain," says Dr. Tammy Mondry, a physical therapist in San Diego, CA, who is certified by the Lymphology Association of North America in lymphedema treatment. Most of Dr. Mondry's patients are breast cancer survivors, though a significant portion developed lymphedema after gynecological cancer, prostate cancer, or melanoma treatment.
Dr. Mondry performs manual lymph drainage to stimulate the pumping rate of the healthy lymph vessels throughout the body. This is one part of a four-part treatment called Complete Decongestive Therapy, which is performed 5 days a week for 2 to 4 weeks and includes compression bandaging with short stretch bandages that are worn 24 hours per day until the decrease in swelling plateaus. After that, the patient wears a compression garment daily for maintenance.
Dr. Mondry teaches people how to perform this massage on their own, as well as exercises and skin and nail care, which are especially important because the lymph that builds up in swollen limbs increases the risk for and severity of infections.
Dr. Paskett and other researchers are looking at ways to prevent lymphedema in women who receive a full axillary node dissection as part of breast cancer treatment, so that these patients can be provided with information that can keep them aware of the symptoms and allow them to obtain treatment quickly.
Awareness and diligence are crucial, because "the risk for lymphedema never goes away after surgery," explains Dr. Paskett. "We mainly see it develop in the first 12 to 18 months, but new cases continue to happen over time."
— Brittany Moya del Pino
Targeted Therapy for Lymphoid Cancers
Name of the Trial
Principal Investigator
Why This Trial Is Important
A new drug called ABT-263 may block the activity of Bcl-2, thereby allowing cancer cells that depend on this protein for survival to undergo apoptosis. Preclinical studies have shown that ABT-263 can bind to Bcl-2 in cancer cells and prevent it from functioning, leading to cell death.
In this clinical trial, patients with T-cell or B-cell lymphoid cancers that have recurred or progressed despite prior chemotherapy will receive ABT-263 orally for up to a year. Researchers seek to establish the maximum tolerated dose of ABT-263 and to determine the drug's safety and, preliminarily, its effectiveness in these patients. They will also study ABT-263's activity in the body (pharmacokinetics).
"The Bcl-2 protein family plays a crucial role in cancer cell immortalization in B-cell lymphomas, as well as in some T-cell lymphomas and solid tumors, making it an important molecular target for these cancers," said Dr. Wilson. "This is the first study in humans of ABT-263, which was specifically designed to inhibit Bcl-2."
Who Can Join This Trial
Study Site and Contact Information
An archive of "Featured Clinical Trial" columns is available at http://cancer.gov/clinicaltrials/ft-all-featured-trials.
NCI Participates in Two Congressional Events
NCI Director Dr. John E. Niederhuber participated with four other NIH Institute and Center directors in a Senate hearing of the Appropriations Subcommittee on Labor-HHS-Education on May 21. The theme of the hearing was "Vision of the Future - Health and Medicine (Predictive and Preemptive)." Dr. Niederhuber described the progress NCI has made in cancer research, including advances being made in gene analysis and the increasing cost effectiveness of such tests. Senators' questions for Dr. Niederhuber focused on the development of advanced technologies for early detection and treatment of disease and improving community access to care. Dr. Niederhuber's testimony highlighted some of the exciting opportunities NCI researchers are pursuing; his prepared remarks are available at http://www.cancer.gov/aboutnci/Senate-Subcommittee-on-Labor-HHS-Education-Appropriations.
On May 24, Senator Barbara Mikulski (D-MD) invited all female Senators to convene for a special public meeting on mammography, held in light of a recent NCI study published in Cancer, which reported a 4 percent decrease in screening since 2000. Dr. Niederhuber and Dr. Nancy Breen of NCI's Division of Cancer Control and Population Sciences were joined by a panel of experts from the Centers for Disease Control and Prevention, the American Cancer Society, and Susan G. Komen for the Cure to discuss the new statistical findings and some of the potential causes to be studied further.
If Memory Serves...
In the 1930s, surgery, radiation, and x-rays were the only treatments available for cancer. In its earliest years, NCI began distributing radium to about 70 hospitals throughout the country for the treatment of financially disadvantaged patients. This program lasted through the late 1960s and was favored by Congress because it brought immediate benefits to the American public. Read more)
For more information about the birth of NCI, go to http://www. cancer.gov/aboutnci/ncia.
Hoover Presents NIH Gordon Epidemiology Lecture
Dr. Hoover reviewed the evidence for hormonally related breast cancer risk factors, some of which have run counter to prevailing hypotheses.
The lecture is named for Dr. Robert S. Gordon, Jr., the first director of the NIH Office of Disease Prevention. The event is available for viewing at http://videocast.nih.gov/.
American Society for Microbiology Honors Gottesman
Dr. Gottesman was an editor of ASM's Journal of Bacteriology and she served as the chair of both the Division of Genetics and Molecular Biology and the Ethical Practices Committee.
ASM presented the award during their general meeting, May 21-25, in Toronto, Canada.
Teleconference Focuses on Cancer Centers Program
For additional information, go to http://ola.cancer.gov/activities/teleconferences or contact NCI's Office of Liaison Activities at 301-594-3194 or email@example.com.
DCCPS Report Available Online
May 31 is World No Tobacco Day
Meet the Experts
Saturday, June 2
Sunday, June 3
Monday, June 4
Featured at the NCI Exhibit
cancer Biomedical Informatics Grid (caBIG)
Metastasis Comes Into Focus at CCR
For much of the 20th century, a diagnosis of cancer terrified many who received it. Today, after some four decades of research progress in cancer detection, control, and treatment, the term that should cause more concern than "cancer" is "metastasis." Many oncologists agree that metastasis, not the primary tumor, is what kills most people who die from the disease.
"By the time we see patients in a clinic, the metastatic cells have often already spread," says Dr. Kent Hunter, chairperson of the Metastasis Working Group in NCI's Center for Cancer Research (CCR). Unfortunately, currently available imaging technologies cannot detect very small lesions or single metastatic tumor cells. Improved patient survival after application of adjuvant therapy, however, is thought to be due to successful targeting of these subclinical micrometastases.
The metastatic cell has been called the decathlete of tumor cells, says Dr. Hunter, because it must succeed at a succession of different tasks. It has to invade the circulating blood or lymph vessels, avoid attack by the immune system, survive a lack of nutrition and cell-to-cell contact, resist shear forces as it travels, stop at a likely spot, get back into adjacent tissue, and finally establish the blood supply needed to proliferate at this new site.
Many cells begin this journey, though only a few survive it. In theory, the sequential process could be thwarted at any point. However, since tumor dissemination is often an early event, the most likely points of clinical intervention are at the end of the metastatic cascade. "You don't want to target an earlier step and find out the horse has already left the barn," says Dr. Hunter.
"Metastatic cells may be quite susceptible to new treatments early after their arrival at secondary sites and as they begin to proliferate," says Dr. Chand Khanna, head of the Tumor Metastases Section in CCR's Pediatric Oncology Branch. Dr. Khanna and others have been working on antimetastasis approaches based on the role of a cytoskeleton protein called ezrin that contributes to many steps in the metastatic cascade.
"Ezrin is the ERM (ezrin-radixin-moesin) protein about which we know the most," says Dr. Khanna. It sits at the cell membrane and coordinates signals about what's happening outside the cell through the interior structure (the cytoskeleton), resulting in an appropriate cellular response.
Ezrin first emerged from mouse model experiments by Dr. Khanna and Dr. Glenn Merlino, chief of CCR's Laboratory of Cancer Biology and Genetics, conducted a few years ago using a series of osteosarcoma and rhabdomyosarcoma cell lines. "When we isolated the genes involved, ezrin popped out as a player in nearly all of the cell lines that were highly metastatic, but was barely expressed in lines that were poorly metastatic." If ezrin expression proves crucial to metastasis, suppressing it should not be too difficult, and may yield a therapeutic clinical strategy.
CCR Scientific Director for Clinical Research Dr. Lee Helman became very interested because he sees many patients in the Pediatric Oncology Branch with both of these cancers. Osteosarcoma is the most common bone tumor among children - less than 20 percent of patients with metastases will be cured.
He and Dr. Khanna wondered if the ezrin expression pattern seen in the mouse models could be found in people. They obtained tumor tissue from dozens of pediatric osteosarcoma patients and the data were clear. "Ezrin was more plentiful in the patients whose disease was highly metastatic compared with those that were not. Understanding the connections and pathways that include ezrin may be a major therapeutic opportunity in a disease we really want to go after," says Dr. Helman.
Dr. Patricia Steeg, head of the Women's Cancers Section in CCR's Laboratory of Molecular Pharmacology, is also unraveling the mechanics of metastasis. Her discovery of Nm23, the first metastasis suppressor gene, pointed the way to a dozen others and opened up a new class of experimental models, leading to a translational strategy. "We're looking at a compound to elevate Nm23 in micrometastatic tumor cells," she explains, with the hope of interfering with colonization in high-risk cancer patients.
— Addison Greenwood


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Lesbian, Gay, Bisexual, Transgender Peace Corps Alumni
Peace Corps at PRIDE Events Across the Country!
Are you interested in learning more about the Peace Corps and how we support LGBTQ volunteers and staff?
Staff from the US Peace Corps will be on hand this summer at more than a dozen Pride events. Returned Peace Corps Volunteers will join them and share their stories and answer your questions. Watch for them in parades and drop by the booths to pick up some information about how you can live and work abroad as a Peace Corps volunteer! For a complete list of Pride Events where Peace Corps will be present see PRIDE EVENTS on the Peace Corps website.
Welcome to the Adventure of Your Life!
We promote Peace Corps ideals and the legal, political and social rights of gay, lesbian, bisexual and transgender people around the world.
Who we are …
We are an organization of gay, lesbian, bisexual and transgender people and others who are Peace Corps volunteer alumni, current volunteers, former and current staff members and friends. Founded in Washington D.C. in 1991, we have members throughout the country and around the world who have served in Peace Corps since its beginning in 1961. We're made up of a national steering committee, together with regional chapters. We are an active affiliate member of the National Peace Corps Association.
Contribute an Article
We invite current volunteers, recently returned volunteers and our longstanding RPCV members to write articles about issues, concerns and activities that are compatible with our Mission Statement. "We promote Peace Corps ideals and the legal, political and social rights of gays, lesbians, bisexuals and transgender people around the world." Contact editor Mike Learned with articles, ideas for articles, editorial comment and any questions.
Lesbian, Gay, Bisexual and Transgender RPCVs are a proud supporter of the United States Peace Corps and an affiliate of the National Peace Corps Association, the official Peace Corps alumni organization.

subset: commoncrawl


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Originally were married 27 April 1951, had our family, but divorced in 1967. Found each other again in 1998 and now committed to each other from this day on.
(Steve Huckins/AP)
Police deliver pie after Pizza Hut driver hurt
PORTLAND, Ore. (AP) — After a pizza delivery driver was injured in a crash in Portland, Oregon, two police officers completed the delivery for him. Full Story
Pictures shows aging, bald imprisoned music legend
LOS ANGELES (AP) — Two newly released photos of Phil Spector show the toll of age and prison on the once flamboyant music legend. Full Story
(RONALDO SCHEMIDT/AFP/Getty Image)
Mark Zuckerberg ticks off San Francisco neighbors
Facebook founder's massive home restoration involves 40 to 50 workers and has been under way for more than a year.


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Free English conversation classes at Manchester Art Gallery.
English Corner at Manchester Art Gallery is for people who are learning to speak English.
Our English tutor will lead you in activities that will help you practise your speaking and listening skills.
No need to book.
If you would like to get an English Corner email reminder every month, please contact Ruth Edson
Email: firstname.lastname@example.com
Tel: 0161 235 8877.


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Show & Tell
What is she?
Please note comments are moderated. After being approved your comment will appear below.
Submitted by adale in louisv... | March 6 2012 | Permalink
Submitted by Loma | March 6 2012 | Permalink
Had a dog just like this years ago and it was a Golden Retriever mixed with an Old English Sheep Dog. He was snow wire, had fuffy, wire like hair and shed like crazy. He weighed over 100 lbs. Beautiful dog.
Submitted by Nina L. | March 7 2012 | Permalink
100% goodness is the breed. If you are really curious you can take a swab of your pup's saliva and do a DNA test. There is a company that specializes in identifying the breeds in mutts. Not sure of the e address - but I bet someone out there knows.
Submitted by Darlene | March 8 2012 | Permalink
I have an 18-pound mix from Mexico. She is black and white, has long floppy ears, long hair, part of it curly, pointed nose, climbs trees, and looks like a distant relative of a Tibetian Terrier. I had 2 DNA tests done. 1st one came back as: sheltie, great pyrenees, and papillion. Nope. 2nd one: samoyed, japanese chin, and chihuahua. Nope. So I gave up and just love her to death...but I still wonder what she could be.
Submitted by Debbi | March 14 2012 | Permalink
If the picture is accurate, I'm betting on some mastiff also (or bulldog) due to the shorter muzzle and close fitting ears. Otherwise...??? Golden retriver? Terrier? Great Pyrenees? She sure looks like a cutie, whatever she is. :)
Submitted by Joy | March 27 2012 | Permalink
In my opinion, the definitive answer is, "The doggie is from a good family". Nuff said....
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There's no easier and more affordable way to get started on electric guitar than this value-filled Squier® set. Opening this box opens a whole new musical future for you, with a great-sounding, cool-looking and easy-playing Affinity Series™ Strat® guitar, a compact 10-watt Fender Frontman® 10G amp to plug it into and all the accessories you need, including an electronic tuner, instructional DVD, gig bag, cable, strap and picks.
Squier Affinity Series™ Strat® is available in Black and Brown Sunburst, and features three singing, sparkling single-coil pickups on a classic single-ply white pickguard, rock-solid bolt-on maple neck with rosewood Fingerboard, skirted white control knobs and a sharp new gold-and-black Squier headstock logo. Fender Frontman 10G amp features custom-voiced built-in overdrive for natural grit and adjustable gain control that rocks guitar tones from tube-emulated overdrive to full-strength ultra-saturated distortion (perfect for blues, metal and the famous Fender clean tone), along with volume, treble and bass controls, 6" Special Design speaker, closed-back design for heavier bass response, auxiliary input and headphone jack, and classic black panel/skirted knob/silver grille cloth looks.
You get guitar, amp, accessories and instruction all at once, and more than ever it's everything you need to Stop Dreaming, Start Playing!™
You are welcome to praise or criticize the products you have purchased. However, please be specific in your comments as to why you like or dislike a product, so that it's helpful to others in their decision-making process. All reviews will be monitored, and normally posted within 1-2 business days. Please refrain from including phone numbers, addresses or URLs in your review. Also, we ask that you please do not post promotional content such as advertisements of any kind (including "for sale" ads), links to your music page or music video, or repeated posts that make the same point excessively. Fender reserves the right to reject publication, without notice, of any review due to content, language or other issues.
Note: currently we are only approving reviews written in English.
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owns this item
owns Fender product(s)
I got a set almost exacly like this once and it was everything i needed to start off learning how to play , all begginers should by sets like these
I played an acoustic before but this is my first electric. It's great to learn on and has great sound for the price. Only comment I have is not to buy Fender instructional video because you get an abbreviated version with the package, it's redundant. But buy the Squier to get off to a good start.
Barbara Heffernan owns this item
|Model Name:
|Stop Dreaming, Start Playing!™ Set: Affinity Series™ Strat® with Fender Frontman® 10G Amp, Black, 120V
|Model Number:
|Body Material:
|Body Finish:
|Body Shape:
|Neck Material:
|Neck Finish:
|Neck Shape:
|"C" Shape
|Scale Length:
|25.5" (648 mm)
|Fingerboard Radius:
|9.5" (241 mm)
|Number of Frets:
|Fret Size:
|Medium Jumbo
|String Nut:
|Synthetic Bone
|Nut Width:
|1.6" (40.6 mm)
|Position Inlays:
|Neck Plate:
|4-Bolt Standard
|Bridge Pickup:
|Standard Single-Coil Strat
|Middle Pickup:
|Standard Single-Coil Strat
|Neck Pickup:
|Standard Single-Coil Strat
|Master Volume, Tone 1. (Neck Pickup), Tone 2. (Middle Pickup)
|Pickup Switching:
|5-Position Blade: Position 1. Bridge Pickup, Position 2. Bridge and Middle Pickup, Position 3. Middle Pickup, Position 4. Middle and Neck Pickup, Position 5. Neck Pickup
|Pickup Configuration:
|6-Saddle Vintage-Style Synchronized Tremolo
|Hardware Finish:
|Tremolo Arm Handle:
|Standard Tremolo Arm
|Tuning Machines:
|Standard Die-Cast
|1-Ply White
|Control Knobs:
|White Plastic
|Switch Tips:
|Fender® USA 250L, NPS, (.009-.042 Gauges)
|Included Accessories:
|Electronic tuner, instructional DVD, gig bag, cable, strap and picks.
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Next, you'll be asked to connect with Twitter. Rest assured, we will never send a tweet from your account without your permission.
RT @MrsKiearaJ: @ochocinco Chad can you support my 8 year old daughter business? She make graphic T-Shirts for young girls.
Toine what's up my dude RT @antoine5757: @ochocinco chad u know @Dr_Skyler ain't coming out the valley to go anywhere lol
Okay RT @SHANNON_MAC: @ochocinco Chad - We still gotta race. Hey - check this out...Need to get you in. Talk to me!
You forgot the @ RT @ErThangRed: Man Chad avi 😍😍😍😍😍
Yo son she wants you 😁 RT @_indecisive1: @ochocinco Thanks a lot Chad.
Lmao 😂😂😂 RT @retrorobb: @ochocinco I'm in tears chad 😂😭
In exchange you must post more women over 140 pounds... please RT @PostBadBitches: @ochocinco shout us out
2 so far, really surprises women when you can play RT @DaQuanBowers91: I gotta see you with a ax lmao .. How many guitars you have
What you think about M&M's and Skittles? Different taste but fucking good RT @J_PREZZ: @ochocinco What do u think of Puerto Rican Women?
Grabbed @kanyewest "Cruel Summer" in black also, giving her/them a choice. Women love making choices
Of course not, church and the dentist office RT @colesams13: @ochocinco do you only use twitter in an attempt to speak with random women?
Even if i sent it to a random woman or nah? RT @VuhJynaSaurus: News flash: women are not flattered by unwanted dick pics from strangers..
My bad, go back in class RT @JKfBaby93: I just had to leave class cause @ochocinco had me laughing so hard with these tweets...
$20 class RT @AnaErviti: Well, my 6 pack and cockiness could use some perfecting...RT"@ochocinco: With me RT @AnaErviti: Practice perfection
I'm done working on a 10 pack... i now await Barca/Bayern match RT @ZGrenier42: I just got out of class. What's on your agenda for today?
😂 RT @MARLONLWAYANS: full body work out,core and endurance Shit makes u sweat the liquor OUT bro. But the class smell like lukewarm asshole
I've been the topic of many classes mainly my "Marketing" n "Branding" RT @KLucas26: Talking about @ochocinco in class right now
In class? RT @BlagoBlogger1: in class, we watched the video, when you took out your fans for a steak dinner #respect #classact
😁 RT @mslauralinda: Man @ochocinco uses twitter in one of the most...unique ways I've ever seen lol
See ----> RT @AgrazAmber: When I'm bored, I just get on Twitter & read @ochocinco 's tweets. #priceless #entertainment
You'll lose RT @Scarkillaz: @ochocinco YES I'm calling you out on twitter to play you in FIFA #ps3... you can even stream it on #youstream!
I was on Black Planet and My Space RT @candleman75: Yo @ochocinco how did you function BEFORE twitter??
Are there any professional cyclist on twitter that are in the Miami area that'll allow me to use a bike for Critical Mass tonight?
Of course not, church and the dentist office RT @colesams13: @ochocinco do you only use twitter in an attempt to speak with random women?
Hell no but since I made them being part of Mothers Day possible I'm entitled 2 thank u sex RT @bigtymer90: @ochocinco u regret havin kids?
Don't listen to him, i've been on 225 dates n 2 months RT @cdat: @ochocinco @rock9449 terrible idea. Don't play games. Games are for kids
Anytime your child/kids hit you with that "I Love You" out the blue it's the setup for the "I need money" question
Small example: the kids played A$AP Rocky song "F**ckin Problems" and knew every word but there was an awkward silence in many verses 😬
Damn right my kids get grounded if they lose, no reason to lose with my genes shit RT @Lez_E: @ochocinco and if yo kids lose you ground them
Anytime RT @jasonsmoon: @ochocinco Thanks for taking a picture with my kids this afternoon. You made their day. You're a class act.
Leigh Bodden/Revis, patient n that damn arm bar RT @YouRecreant: Revis is like God in the CB position though. Anyone else struggles with you
I do every morning and he says be yourself and not a pretentious robot for society RT @crowbaby12: @ochocinco you need to talk to GOD bro
Are u flirting? I've been hurt, don't do that RT @FeministaJones: Can't forget a gift from God! RT @ochocinco someone remembers my existence
Nah Moses did most of the work RT @Baddest_Indeed: @ochocinco God himself .
Don't agree with the title Black Sabbath chose for their single but I love the song "God is Dead" album isn't out for a while though...
😡 RT @Siiiimonsays: If you claim god exists you need to prove it or you can assume everyone thinks you also believe in santa claus. Moron.
Dude stop playing around, that's impossible... RT @mdoscher2: @ochocinco try 180K after 6 years of Pharmacy school
Hope you get an academic scholarship, college cost sh*t RT @Jicyraa_Carter: 😭 child please.. school is my only job until i graduate college
This is my last tweet about school, what about the moment you walk in class and notice you have a substitute teacher though? Shit I was out
Remember in the movie "Lean On Me" when Joe Clark caught Sams n his friends in the bathroom, I appreciate that school song moment...💯
Remember in school when you'd be happy just to go to the bathroom to get out class. Those simple moments were priceless right?
Using pandora playing Lil Boosie's song "Do the ratchet" holding my phone next to the Orca's tank and she's splashing to the beat #Dope
Small example: the kids played A$AP Rocky song "F**ckin Problems" and knew every word but there was an awkward silence in many verses 😬
Sitting in McDonalds parking lot eating pancakes listening to The Commodores song "Just to be Close to You" n the beginning words are EPIC
I knew i wasn't crazy RT @knosHERworth: I cry when I listen to that song too! It's okay! We are just in tune w/ our emotions bruh😜
RT @_MsWilliams: Chad Johnson said that song called Suitcase by Emeli Sande made him cry, and I just listened to it and did the same thing. @ochocinco
I just cried before my workout (my emotional ass) listening 2 Emile Sandè song entitled "Suitcase" I pulled myself together though, I'm good
Soon as you're single RT @OhItsDindi: Just me and my boo @ochocinco in Miami, we need to take more pics together ♡
Bump as in run into or is bump a sexual related term in London RT @EmaLouiseH: Wish I was in Miami to be able to bump into @ochocinco
Hello I'm in Miami as well and I'm meetable (is that a word) anyway i'm free RT @IAMPORSCHA: @ochocinco hey im in miami i want too meet u
Where are you? RT @Rubyeautiful: In Miami! What's for dinner @ochocinco??
Heat/Clippers and we'll be court-side no lol RT @CariChampion: @ochocinco lolololol. Now you're being funny. Miami and who in the Finals?
😂😂😂😂 RT @itschuco: Do they make u pay? @ochocinco #Miami #DavidsCafe
😵 RT @reIoweded: I waited until mine went on vacation RT "@petitely: 😂 RT @A2daO: Your girl went on vacation specifically to cheat on you"
😂😂 RT @HeezyBear: You're a real scumbag if you go to a different barber for a week. Cheat on your girl if you must cheat, not your barber.
Would a sweet Mormon girl love a bad boy is the question? RT @jessleav: @ochocinco Chad, would you ever love a sweet Mormon girl?
RT @supe3man: Girl came home last night with blockbuster movies talking cuddle ish like the nba playoffs not on. Hit her with that @ochocinco #chileplease
Miami guy loving a Boston girl virtually... :) RT @chrisjan36: @ochocinco.....Boston girl saying hi....not that you ever say hi back ;)
Like Boyz 2 Men/New Edition or nah RT @DamnnEricka: if one girl knows A LOT of guys that are in the same group what does that make her?


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We know the dilemma all too wellyou want to add some drama to your trademark hairstyle, but don't have time for a salon appointment. The answer? A faint tint like the red shade Aishwarya Rai wore. It's an easy way to try out a new look with minimal effort, and even better, the effect is easy to create at home. If your strands are dark like the star's, try out a red gloss, or a color-depositing conditioner. Not only will you get an instant change, but both products restore the shine that may have been lost through heat styling, and provide a needed boost in hydration. If you have lighter hair, opt instead for an at-home gloss as color-depositing conditioners tend to stain fair tones, and veer no more than two shades lighter or darker than your natural huea professional stylist will be better-equipped if you want a more dramatic switch.
Star-Inspired Holiday Hairstyles
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Hyundai Sonata GPS Navigation
Customer Reviews
Posted by Joan S.
"I bought the Garmin GPS 1390t after borrowing my fathers for a few days for work. And wow I love this thing gets to my destination quickly and easily. Everything about this device is so easy to use. I couldn't live without it the best money I have ever spent."
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Code Lyoko Ulrich ShirtProduct Sku: CLK009
$ 12.00
- This Code Lyoko Ulrich shirt features quiet but powerful warrior Ulrich Stern.
Size Width (A) Length (B) Small 18" 26.5" Medium 20.5" 28.5" Large 22.5" 30.5" XL 24" 32" 2XL 26" 32.5" 3XL 28" 33"
*Measurements are listed in US Inches.
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- • Product Type: T-shirts
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• Gender: Mens
• Graphic: Heat Transfer
Distressed refers to the worn appearance in the image or lettering on a garment. A distressed print may include faded areas, missing color, or other irregularities. This is an intentional occurrence in the product and adds to the vintage look of the design.• Tag Location: TaglessThe traditional size tag has been removed from the neck seam. Sizing and composition information is screen printed on the inside of the back of the shirt, just below the neck seam or collar. Popular clothing brands often use tagless t-shirts for extra comfort when wearing.
• Made of: 100% Cotton
• Fits like: True To Size
• Fabric Softness: StandardT-shirts of STANDARD SOFTNESS are composed of traditional material and are considered to be your standard weight classic T-shirts.


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Starbucks® Stainless Steel & Ceramic Tumbler - Purple, 12 fl oz
A tumbler with a stainless steel exterior, ceramic inner lining and rich purple finish.
- Product Details
Starbucks® Stainless Steel & Ceramic Tumbler - Purple, 12 fl oz
Enjoy the best of both worlds with this Stainless & Ceramic Tumbler with the imperial purple finish. The stainless steel exterior helps keep hot beverages warm and hands cool, while the ceramic inner liner offers the satisfying experience of sipping coffee from your favorite coffee mug. Now that's a proper cup. Featuring a press-in lid with silicone seal to help minimize spills, the coffee tumbler holds up to 12 fl oz. Starbucks name on side. Hand wash only. Do not microwave.


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Minn Kota Fortrex - 80 lb Thrust, 52" Shaft, 24V, US2 - Foot Control
Product Families
With fewer moving parts, tighter tolerances and less give, Fortrex is the beefiest, meanest, quietest motor ever. Fortrex's innovative Mono-Arm Design features an extruded, anodized aluminum structural beam that operates with no metal-to-metal contact and 75% less torsion. Counter-Tension Stabilizer uses an integrated spring that applies force whether the motor's stowed or deployed. On the water, that translates to a motor with no play, no give, reduced steering torque and silent operation. Lift-Assist eases stow and deploy.
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18 U.S. Code § 3557 - Review of a sentence
Source(Added Pub. L. 98–473, title II, § 212(a)(2),Oct. 12, 1984, 98 Stat. 1991.)
Effective Date
Section effective Nov. 1, 1987, and applicable only to offenses committed after the taking effect of this section, see section 235(a)(1) ofPub. L. 98–473, set out as a note under section 3551 of this title.
The table below lists the classification updates, since Jan. 3, 2012, for this section. Updates to a broader range of sections may be found at the update page for containing chapter, title, etc.
The most recent Classification Table update that we have noticed was Tuesday, August 13, 2013
An empty table indicates that we see no relevant changes listed in the classification tables. If you suspect that our system may be missing something, please double-check with the Office of the Law Revision Counsel.
|Description of Change
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Melissa's Q & A
Who's your favorite sports team, and why?
I love college football. My favorite team is the Florida Gators because they've been my favorite since I was a little girl, and Tim Tebow definitely helped evolve my love for them. Regardless, with him gone, they will always have me as a number one fan.
What's your favorite movie quote?
Brennan Huff: I swear, I'm so pissed off at my mom. As soon as she's of age, I'm putting her in a home. STEP BROTHERS!
What's your favorite video game, and could you kick our butts at it?
YES. I am a pro at Call of Duty :Zombies on Xbox! You can say I'm OBSESSED with it. Wait until I get a hold of the HAMR and the monkey bomb. It's over with!! Also Assassin's Creed - HANDS down best game series EVER. I LOVE the cinematic features/stories of these games.
Piercings/Tattoos (How many? Where?)
My ears are pierced twice, and I also have my belly button pierced but I took it out. I grew out of it.
What's the most embarrassing song on your iPod?
Call me maybe! Isn't that everybody's?
What's your best party trick?
What's the most memorable pick-up line you've ever heard?
"Believe it or not, I wasn't always as awesome as I am today." (After buying me a drink, it's off of How I Met Your Mother) So, it was extremely memorable and so funny. I love pick-up lines. The cheesier, the better!
What's the worst job you've ever had?
Working in a factory lifting huge boxes and killing my back!
What's the most dangerous thing you've ever done?
Jumping off of cliffs during summer time. It's definitely illegal now, and I LOVE it!
It's 4AM after a crazy night out – what are we eating?
A concoction of McDonald's Breakfast, Doritos, then before I fall asleep, probably some chocolate chip cookie dough and milk. I can't survive without milk.
What's the strangest thing in your fridge right now?
Homemade dog treats? I love my pit bull! :)
What's the naughtiest thing you've ever done in public?
I never kiss and tell :)
What do you feel sexiest wearing?
I feel sexiest wearing a matching bra and panties outfit. Anything lingerie.


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Portable nebulizers are slightly larger than a deck of cards, so they can be carried in a purse, briefcase, or backpack to be used whenever and wherever you need them.
The Omron CompAir Elite Portable Compressor Nebulizer is a compact nebulizer that provides treatment of allergies, asthma, and other respiratory disorders anywhere you need it. The upgraded features to the Omron CompAir Elite Portable Compressor Nebulizer have made the perfect nebulizer you can take with you where ever you go! The unique low flow technology of the Omron CompAir Elite Portable Compressor Nebulizer allows safe and convenient treatment almost anywhere. This compact compressor nebulizer also can run on AC or Batteries with the optional rechargeable battery package.
The Omron NE-U22V MicroAir Nebulizer System is the first portable nebulizer with a powerful delivery that is comparable to table-top compressor nebulizers. Designed for patients suffering from Asthma, COPD, or any other respiratory conditions, the Omron NE-U22V MicroAir Nebulizer System delivers undiluted medication, shortening treatment time so you have more time to do the things you love. The Omron NE-U22V MicroAir Nebulizer System is one of the few compressor nebulizers that is tubeless and cordless providing an unmatched portability and ease of use.
The Respironics MiniElite Compressor Nebulizer System is a fast, portable nebulizer that optimizes treatments and promotes active lifestyles. Easy to use, transport, and maintain, the Mini Elite Compressor Nebulizer System nebulizes most commonly prescribed solutions and suspensions. The compressor nebulizer system includes: MiniElite Compressor, Micro Plus reusable nebulizer handset, AC electrical adapter, DC electrical Car Adapter, four filters, instructional DVD, and a carrying case. The unit only weighs 1 lb.
Nebulizers are electric or battery-powered medical devices that convert liquid asthma medicine into a fine mist that's inhaled into the lungs. The user breathes in the mist through a mouthpiece or facemask. Nebulizers vary in size and shape, but can be a bit bulky and noisy and may need to be plugged in. Nebulizers are commonly used for the treatment of cystic fibrosis, asthma, COPD and other respiratory diseases. Nebulizers use oxygen, compressed air or ultrasonic power to break up medical solutions and suspensions into small aerosol droplets that can be directly inhaled from the mouthpiece of the device.
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Download the KTIV Storm Team 4 Weather App for Android and Apple (iOS) devices and track the storm.
Get the free "KTIV Storm Team 4" weather app now. You'll enjoy interactive radar with storm tracking tools, video forecasts, severe weather push alerts and much more. It's now available for Android and iOS phones and tablets.
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By Jonathan Henschen, CFS |
May 22, 2013
Whether you're a high-end wine or a high-end broker-dealer with a cult following, it's all about "the experience."
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Browse the latest stories and reports on MSF from various media outlets.
Filter by vocabulary: issues (multisite):
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- Disease (17) Apply Disease filter
- HEALTH (17) Apply HEALTH filter
- Infectious Disease (17) Apply Infectious Disease filter
- HUMANITARIAN CRISIS (3) Apply HUMANITARIAN CRISIS filter
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- Earthquake (2) Apply Earthquake filter
- Cholera Remove Cholera filter (-)
MSF's Duncan McLean explains that Haitians are at worse risk of dying from cholera now than they were two years ago, due to neglect of the country's health care system by international donors.More >
MSF's Duncan McLean explains that Haitians are at worse risk of dying from cholera now than they were two years ago, due to neglect of the country's health care system by international donors.
View external media
MSF's Oliver Schulz explains how a lack of aid is leaving Haitians vulnerable to cholera and other catastrophes.More >
MSF's Yann Libessart describes the poor state of Haiti's cholera treatment centers and the need for international funding of Haiti's cholera elimination plan.More >
MSF reports that the cholera crisis in Haiti is getting worse due to a lack of funding and basic medical supplies.More >
MSF's Oliver Schulz speaks with Thomas Roberts of MSNBC about cholera and other health challenges facing Haiti three years after the earthquake.More >
Medical teams from Doctors Without Borders witnessed a small increase in the number of cholera patients in Port-au-Prince following Hurricane Sandy.More >
Deane Marchbein, president of Doctors Without Borders, spoke about responding to crises in South Sudan, Libya and Haiti on the public radio program "Conversations with Allan Wolper."More >
In the 10 weeks since an epidemic erupted in Haiti, cholera has killed more than 3,000 people -- partly because the distribution of health supplies remains a logistical nightmare.More >
Guest blog by David M. Olson, MD, Medical Advisor, Médecins Sans Frontières/Doctors Without Borders (MSF), New York, NY, USA. This week I have been called back to Haiti because of the cholera epidemic that first hit in late October and which continues to have a devastating effect. At the time of writing this post, more than 1,100 people have died and over 18,000 hospitalizations due to cholera have been reported throughout the country.More >


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Alesone Gray of Cranlegh
From EastKingdomWiki
m (→Service)
m (→Projects & Photos)
|Line 76:
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*Rapier armor http://www.flickr.com/photos/alysten/sets/72157625772638559/
*Rapier armor http://www.flickr.com/photos/alysten/sets/72157625772638559/
*Culinary: http://www.flickr.com/photos/alysten/sets/72157622537504281/
*Culinary: http://www.flickr.com/photos/alysten/sets/72157622537504281/
*General projects
*General projects : http://www.flickr.com/photos/alysten/sets//
*General projects 2010: http://www.flickr.com/photos/alysten/sets/72157624433690725/
*General projects 2010: http://www.flickr.com/photos/alysten/sets/72157624433690725/
== More Information ==
== More Information ==
Revision as of 22:38, 20 March 2011
|This article is a stub. You can help the East Kingdom wiki by expanding it.
Don't know what information to put? See our Templates Page.
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THL Alesone Gray of Cranlegh
Quarterly gules and sable, on a bend sinister argent three fleurs-de-lys gules
|Awards & Offices
Rapier fighter, costumer, cook and confectioner with the Tadcaster Militia, Cadet to Don Ian Raven of Tadcaster.
Alesone Gray, is the daughter of a successful merchant that made a sizable donation to the crown and received a Knighthood. She grew up in Cranlegh village, Surrey, England, as the youngest sibling with five older brothers, in the later part of the 16th century. She was taught dancing at an early age, by the master hired to teach her brothers fencing. She would secretly watch the fencing lessons, longing to play with the blades. After being caught on numerous occasions with weapons in hand, her father allowed her to take lessons with her brothers. She eventually cajoled her father into allowing her to be trained to use black powder and cannons.
Offices & Positions
- Queen's Guard, 9/2008-4/2009
- Kingdom Webminister,10/01/2009 to present day
- Rapier Marshal at Large, Bhakail, current
- Chronicler, Bhakail, 12/01/2010 to present day
- Cook/Water coordinator- Ducal Challenge, June 2009
- Cook- Queen's Tea, Pennsic 2009
- Head cook- Court of Love, Pennsic 2009
- Head cook- Raze a Village, September 2009, 2010
- Head cook- Bhakail Yule, December 2009, 2010
- Cook- State Dinner Pennsic 2010
- Deputy cook- Coronation, September 2010
- Head cook- Mudthaw, March 2011
- Cook- Crown Tourney, May 2011 (scheduled)
- Deputy steward- Raze a Village September 2009
- Event steward- Noisemakers, March 2010, February 2011
- Royal Liaison- Ducal Challenge, June 2010
- Pennsic 2009, 2010, 2011(scheduled)
- River War, September 2009
- Known World Academy of Rapier/Known World Costume Symposium, October 2009
- Cooks Colliegium, January 2010
- Royal University of the Midrealm November 2010, 2011(scheduled)
- Cooking Thynge, March 2011
- Southern Region War Camp, April 2011(scheduled)
Rapier Armor:
- HRM HRM Alethea I, 2008
- HRM Andreas IV, 2009
- HRM Konrad II, 2009
- HRM Margarite I, 2010
- HRM Gryffith II, 2010
- HRH Lucan VIII, 2011 (in progress)
Class Topics
Costuming- Heraldic Clothing for the Lists, Clone yourself- How to make a duct tape double,How to make and use a drop tester, List legal fencing armor
Cooking- Creating Order out of Chaos- Feasts for 20 or 200, Period Spices, Preparing poultry for Galantines, Secrets of a Restaurant Chef, Food Challenges in SCA Cooking
Confectionery- Large Pastry Structures, Sugar Work/Gum Paste
Miscellaneous- UI Basics for Webministers, Life in the SCA after Gastric Bypass
Projects & Photos
Project journal and diaries http://alysten.wordpress.com
Photo journals
- Rapier armor http://www.flickr.com/photos/alysten/sets/72157625772638559/
- Culinary: http://www.flickr.com/photos/alysten/sets/72157622537504281/
- General projects 2011: http://www.flickr.com/photos/alysten/sets/72157626310726874/
- General projects 2010: http://www.flickr.com/photos/alysten/sets/72157624433690725/
- General projects 2009: http://www.flickr.com/photos/alysten/sets/72157620681340853/
More Information


text:
From WENN.com
The Superbad star was said to have landed the lead role in an upcoming reboot of the series, which starred David Hasselhoff and Pamela Anderson, but his spokesperson has moved swiftly to shoot down the rumours.
His rep has branded the speculation ""completely incorrect"" and tells GossipCop.com that Hader is ""not attached"" to the project.
Hasselhoff revealed last year (12) that he plans to play himself in the film instead of revisiting his role as lifeguard Mitch Buchannon.


text:
|Anime >> English-translated Anime
|Flag torrent
|[Commie] Trigun the Movie - Badlands Rumble [BD 1080p AAC] [E7AC1F12].mkv
|0 fans.
|File size:
|8.52 GiB
Vuze with the Mainline DHT plugin is the recommended client.
Playback help is here, and you probably need it.
Torrent description:
Files in torrent:Show files
User comments:
2011-01-01 at 04:48 UTC
2011-01-01 at 04:48 UTC
does this one have subtitles?
2011-01-01 at 04:59 UTC
2011-01-01 at 04:59 UTC
When it is in the category "English-translated Anime", I strongly suspect so
Dark form10
2011-01-01 at 05:19 UTC
2011-01-01 at 05:19 UTC
2011-01-01 at 06:01 UTC
2011-01-01 at 06:01 UTC
anyone nice enough to upload the subs alone? Don't rly want to redownload 8gb =/
2011-01-01 at 07:02 UTC
2011-01-01 at 07:02 UTC
4 of the original voice actors for the 4 main characters from the original TV series are back! Can't wait! :D
2011-01-02 at 15:35 UTC
2011-01-02 at 15:35 UTC
*sigh* I hope there is a release for people with slower machines.
2011-01-04 at 00:00 UTC
2011-01-04 at 00:00 UTC
it is too large
2011-01-04 at 21:52 UTC
2011-01-04 at 21:52 UTC
Nice thanks. 1080p ftw as long as they don't contain FLAC(which IMO is a waste of b/w).
2011-01-08 at 12:01 UTC
2011-01-08 at 12:01 UTC
I downloaded it but I don't hear people talking in it, only what I hear are the sounds and music :/
2011-01-08 at 13:30 UTC
2011-01-08 at 13:30 UTC
It's 5.1. Get a better decoder.
2011-09-15 at 16:07 UTC
2011-09-15 at 16:07 UTC
Nice thanks! The bigger the better ^^
2013-09-10 at 20:52 UTC
2013-09-10 at 20:52 UTC
thank you for this BD version
Post a comment:You need to be logged in to post a comment.
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[Omnicat] <Nyaa> We will of course be playing Star Citizen. If you have a game package, you can join our subversion efforts right here. See you in the 'verse!
[10-02, 16:28] <stinky> 10-02, 03:38] <stinky> I could use a program to capture hardsubs, but they are filled with errors, and it takes me longer time to fix all that, than to just let Google Translate to do the job through Subtitle Edit. [10-02, 03:35] <stinky> Really, well I don't know russian, and it does a good job of giving me a nice start, and it also does the timings, so, if you got time to waste you can go learn german and translate for me ok. Thanks. I guess the Germans and Russians who translated it are all idiots too huh? [10-02, 03:31] <sarachikorita> Only idiots, kooks and trolls release subs made with machine translators. [10-02, 03:31] <Tysonblast> who is your nt mod waifu, chatbox? [10-02, 03:23] <Artemix> Herkz please delete sinky account. Thanks. <stinky> Herkz= admin troll. He'll erase my argument, and leave the negative comments with no context, so you take them out of contex. [10-02, 03:04] <stinky> Herkz, i asked if anyone knew of a good program to translate subs, you erased that. Then I said I found a good program, Subtitle Edit. You erased that. So asking a question gets erased, like wanting to start a normal conversation. But typing FU Nyaa and you leave it? Just shows who the trolls are, leaving my negative comment so you can act all politically correct and call me troll. Who's the troll? [10-02, 03:01] <stinky> I love it i type, Subtitle edit does a great job at translating subs using the Google Translator, and they erase that. I type FU Nyaa after they do that and they leave that!!?! Whaaa??? Now I can translate a nice version of Wangan Midnight I just found!
[10-02, 16:28] <stinky> it went something like this
[10-02, 16:13] <hazardous> check here: http://anichart.net/airing
[10-02, 14:57] <Mayobe> Chewey, you may want to wait for the first episode to air first.
[10-02, 14:48] <patlabor> ok
[10-02, 13:04] <jofs> @rabcor: Bakabt
[10-02, 12:45] <nitekatt> you can look for that on common torrent trackers
[10-02, 12:37] <rabcor> By Japanese audio I really meant Korean audio :P It's a Korean movie.
[10-02, 12:33] <rabcor> Hey guys, anyone have that Wonderful Days movie from 2003 with Japanese audio? I want it :( only torrent I can find (SEA FS) has no seeds.
[10-02, 12:29] <Chewey> Guys who know when to be sub to названием Danna ga Nani o Itteiru ka Wakaranai Ken(I Can't Understand What My Husband Is Saying)?
[10-02, 12:24] <Dhruv2930> I really don't get it.... Why can't I upload anything?? all I get is a poem ater i click on upload
[10-02, 12:15] <[WolfPack]> OMG hi nyaa personificated. Thank you so much for being back online! (And I apologize for being crazy, even though it's so much fun) Maybe he's mean because he's hungry.
[10-02, 11:34] <Keigo Kakiguchi> so whos gonna sub reconguista in g?
[10-02, 09:12] <Nyaa> I didn't erase your post, stinky. Why are you being rude?
[10-02, 08:48] <Crixitron> Hi guys does anyone know where to download boku wa tomodachi ga sukunai live on stage not live action?
[10-02, 07:51] <sarachikorita> For the record, I've never gotten the teapot error.
[10-02, 05:58] <[TRVE]> That was me. And as far as I know, there is no surefire way to find out what's wrong, it might be anything You can simply try again a few times or you can change something and try again, for example, delete the description.
[10-02, 05:48] <[WolfPack]> It wasn't uploaded. It happened to me. Someone said I did something wrong, but I know I didn't. Maybe it was because I took it from kickass.to and added nyaa's trackers, but it worked before. Maybe it's because I already uploaded stuff. Maybe nyaa just plain bugged and was overloaded. That error isn't supposed to exist. The tea pot was supposed to be a joke. It,s not funny. I can't fix an error if I don't know what I do wrong. I just plain gave up. I'm looking forward to paycho pass 2 and log horizon 2
[10-02, 05:37] <theklutch> damn son
[10-02, 05:11] <Dhruv2930> I've got a question regarding uploading to Nyaa.se .... I uploaded this torrent here and when I click "Upload" , a nursery rhyme comes... how do I know my torrent was uploaded?
[10-02, 05:10] <Dhruv2930> hey guys
[10-02, 05:09] <Dhruv2930> hey guys...
[10-02, 03:31] <sarachikorita> Only idiots, kooks and trolls release subs made with machine translators.
[10-02, 03:31] <Tysonblast> who is your nt mod waifu, chatbox?
[10-02, 03:23] <Artemix> Herkz please delete sinky account. Thanks.
[10-02, 02:34] <Chewi105> Motherfucking DATABASE, duuuh
[10-02, 02:24] <Artemix> So, what's worth watching this new season?.
[10-02, 02:11] <herkz> because you're retarded, stinky
[10-02, 02:05] <sarachikorita> I think certain people here would agree with me that googlefished subtitles need to die in a fire.
[10-02, 01:51] <himuraK24> Nyanpasu~
[10-02, 01:46] <Kenaya9> tes
[10-02, 01:35] <stinky> Why do you erase my post on subtitle edit? Fuckyou NYAA! There
[10-01, 23:35] <Addaz> trolololing
[10-01, 22:39] <titannobaka> there is a search bar,you know.
[10-01, 21:44] <whattawaste> Any Tokyo Ghoul blu ray uploads yet?
[10-01, 21:14] <jofs> Omnicat pls
[10-01, 20:58] <kureyonall> nyanpasu. :3
[10-01, 20:10] <zaturama007> NYANPASU~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
[10-01, 20:10] <zaturama007> is the original the light novel?
[10-01, 17:35] <SneakiestNEG> Most of the stuff on nyaa has plenty of seeds.
[10-01, 17:20] <Kawaii!!> Yeah, i guess i'll have to =(
[10-01, 17:17] <SneakiestNEG> Or just wait for the BD rip to come out. Patience ;)
[10-01, 17:08] <Kawaii!!> Thanks... seems to be stalled for a while now, sadly.
[10-01, 17:03] <MisterDonut> FFF
[10-01, 17:00] <Kawaii!!> Is anyone working on the Danganronpa Blu-rays? Just wondering, thanks.
[10-01, 16:15] <hazardous> lol they shud have named the movie Dragged in to The War
[10-01, 16:08] <[TRVE]> Also, nobody cares.
[10-01, 16:08] <[TRVE]> It's based on the light novel, you dipshit. So is the manga.
[10-01, 14:59] <nitekatt> that tinfoil hat looks so good on you
[10-01, 14:51] <hazardous> time travel is real. hidden gov already have it. amnesia is real. you'all in amnesia right now!
[10-01, 14:32] <sakuyabloodwing> everything that isn't time travel = moe shit
[10-01, 13:55] <xell17> I agree. fuck time travel and fuck amnesia, too.
[10-01, 13:54] <memekontol> ooooooooooooooooiiiiiiiiiiiiiiii
[10-01, 13:43] <nitekatt> who cares? all this time travel shit in anime and fiction is getting annoying
[10-01, 13:14] <Profuse> EDGE OF TOMORROW IS BASED ON MANGA "ALL YOU NEED IS KILL"
[10-01, 12:56] <CrimsonTitanRed> Can anybody help me setup this rss feed with my rainmeter theme?
[10-01, 12:50] <shaddrag> My upload speed is literally 50kb/s, I might as well not even try.
[10-01, 12:47] <Profuse> I have a very important annoucement to make, Edge of Tomorrow is based on a manga that's all.
[10-01, 12:38] <sakuyabloodwing> So I wrote a long explination about why people bitching about people not seeding/sharing is fallible but; TL;DR: ISP throttle upload speeds, for many it isn't doable and any encoder/uploader should make proper use of a seedbox which means you don't need to bitch to your fan base.
[10-01, 12:30] <Tysonblast> more like Gundam Seed PLS
[10-01, 12:21] <GoldenCrow> go kill yourself! (did i do it right?)
[10-01, 11:50] <x265> @lotusgg I see, then I'll keep using this aq values for now, I'll be testing with other anime, in order to get "general settings for anime"
[10-01, 10:19] <AttackNeo++> Seed Please
[10-01, 10:02] <sarachikorita> Oh geez, Mediaset...the Italian masters of the Macekre
[10-01, 09:34] <HypnoDisk> Ciao Benjo ti ringrazio follemente per questa tua release, era tanto che cercavo l'intera serie col doppiaggio milanese. Tuttavia devo segnalarti un piccolo errore. L'episoio 006 non ha la traccia Mediaset, la seconda traccia infatti è sempre quella del doppiaggio romano anni 80. Puoi risolvere? (o in caso contrario dove posso recuperare il 6° episodio col doppiaggio Mediset?)
[10-01, 08:40] <[WolfPack]> The anime suggestion I made is the link for the 480p. I LOVE CRAP. It's a wonderful fertilizer. Enjoy~
[10-01, 08:38] <[WolfPack]> Kips, you have made no torrents and I bet you're not sharing either. So I don't see much value in your mere opinion. I was about to make this torrent but it was already made, so I adopted it. Nozaki is hilarious: #603990
[10-01, 08:31] <[WolfPack]> I'm a french canadian, to me a meme is a grandmother, and they're both dead. I make demotivational posters. I have more than 200 but I'm just getting started. I think that the one I shared is relevant here, and quicker than saying it in my usual millions words. Enjoy. It's so freaking cute!
[10-01, 08:18] <Kips> your memes end here
[10-01, 06:51] <[WolfPack]> I actually came here to edit my torrents with a demotivational poster about the importance of sharing. Look at how cute it is! I'm going to eat so I won't get the wonderful insults you're going to use to praise me. Enjoy~ http://bananapoop.com/zextra/imgs/jokes/sharing-torrents-demotivational-wolf.jpg
[10-01, 06:49] <[WolfPack]> Then why are you here and with a member name. *hands over a rope to Mayobe* Come back as a zombie and watch Tokyo Ghoul
[10-01, 06:40] <Mayobe> Anyone who watches anime should hang themselves.
[10-01, 06:14] <jofs> Underwater = Steven Seagal. Hard to kill
[10-01, 05:16] <Abunja> talking about rip, has underwater re-surfaced again? i saw their release of barakamon the other day.
[10-01, 05:13] <Abunja> there is no easier explanation other than that
[10-01, 05:12] <Abunja> rip = death
[10-01, 05:05] <hazardous> encode from lossless = rip. encode from rip = re-encode. encode from re-encode = death.
[10-01, 04:39] <eternia> Anyone who watches 50 MB anime should hang themselves.
[10-01, 04:39] <lotusgg> you understood me well. Using a 1080p to reencode to 720p is still a reencode.
[10-01, 04:36] <sarachikorita> You would do better to encode CR's 1080p video to 720p, instead of using their 720p at all.
[10-01, 04:30] <NoobSubs> Anyone who watches 50MB Anime files should be ashamed of themselves.
[10-01, 04:14] <lae> >implying source has much anything to do with size
[10-01, 03:46] <krucierchan> i do faggot fuckin fight me I like my 50mb animes it saves a lot of space, queer
[10-01, 03:46] <HerbalNekoTea> Some people has brain fart in the chat. lol
[10-01, 03:41] <herkz> lol who the fuck encodes from CR's 720p video
[10-01, 03:17] <lotusgg> using crunchyroll 2pass 1800kbit and reencode it is not incredible at all.
[10-01, 03:16] <lotusgg> @crf if all reencodes are shit, then almost all releases at nyaa are shit.
[10-01, 03:06] <lotusgg> aq2 is still better for economic bitrates tho.
[10-01, 03:05] <lotusgg> @x265 aq1 is better at highet bitrates and with grainy anime, also is meant to be used with higher strenght.
[10-01, 02:29] <crf> All re-encodes are shit.
[10-01, 02:18] <OnDeed> the best thing soviet union did was to die \o/
[10-01, 02:17] <OnDeed> yeah, I wish they would drop it, those trolls
[10-01, 02:04] <x265> http://www.cccp-project.net/ Name is kind of a joke
[10-01, 02:01] <LiQuid> is vlc bad to use ? cuz I have no idea what CCCP is.
[10-01, 01:43] <Senfu> Database, Database
[09-30, 22:59] <x265> Why nyaa should be https? I mean, no personal data involved...
[09-30, 22:58] <SneakiestNEG> If I had a euro for every time someone asked why it is called the 25th anniversary! Any decent extras?
[09-30, 22:55] <SneakiestNEG> Weird nyaa is not https. Never noticed that.
[09-30, 22:50] <SneakiestNEG> I am incredibly old skool Just get mp4, throw it on a flash drive and stick it in a smart tv. Sure tv's are not the best media players but it is good enough.


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Lord/orchestre Metropolitain
Marie-josee Lord - Opera Arias
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For the Anniversary of 20 years of Sunday Morning Concert a special programme is created not only inside the Concertgebouw, but also outside in the open air, at the Museumplein! This will be a great musical party on Radio 4 and at the Museumplein! Liza Ferschtman likes very much the contrasts ...Read more
Jazzics! is the new album of Matangi Quartet and is an ode to jazz from a classical point of view. To celebrate this brand new and special release, Matangi will present the album in Bimhuis, Amsterdam on Wednesday 10th of September. After years of collaboration with a wide variety of jazz-greats ...Read more


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For Women Who Want The Ballerific Life!
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More Call of Duty Veterans Quit Infinity WardModern Warfare 2 lead designer Mackey McCandlish and veteran Call of Duty series programmer Jon Shiring have left developer Infinity Ward, Edge reports, a mere week after another two high-ranking developers quit the Activision-owned studio.
"After almost 6 years at Infinity Ward, I resigned today," wrote Shiring on Twitter. "I'm incredibly proud of everything we accomplished and I'm going to miss everyone." Shiring joined Infinity Ward during Call of Duty 2 while McCandlish was there from Call of Duty.
Their departures came to light on the day that fired Infinity Ward co-founders Vince Zampella and Jason West announced the founding of their new development studio Respawn Entertainment, which has signed an exclusive publishing deal with Activision's rival Electronic Arts through its EA Partners scheme. However, there's no indication yet of McCandlish or Shiring joining Respawn, or the events being connected.
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Quality CareFind out why Mayo Clinic is the right place for your health care. Make an appointment.
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We've seen it happen a few times in the Panhandle just this past week - dangerous flash flooding on the roadways. It's been an issue the City of Amarillo has been trying to address for years. Now, with new floodgates, commissioners hope fewer drivers find themselves up a creek in a flooded underpass.
"When you can't see the base of the roadway, do not drive through standing water," said Amarillo City Commissioner Dr. Brian Eades. "It can be very deceiving."
Deceiving and dangerous, as flooding at the underpass on Tenth Street in Amarillo claimed the life of an Amarillo teenage boy eleven years ago inn 2002.
"It was not apparent when it was raining heavy and you're driving toward one of those railroad underpasses, but there was 14 feet of water," Dr. Eades said. "He was not able to get out and subsequently drowned. Since then, it's been one of the City's missions to get better control of Third and Tenth Street."
The City originally posted flood gauge signs at those underpasses to show drivers when it was too deep to drive through, but even those warnings were not enough as underpasses like the one on Tenth have been known to quickly fill with up to 14 feet of water. Starting this past Monday, June 17, the City began installing floodgates at some of the more dangerous underpasses.
"They will essentially exclude that side of traffic and there will be a warning sign on them," said Eades. "It'll be quite a struggle to get around those in a vehicle."
The gates will function much like railroad crossings are being paid for with money the City gained through red light cameras.
"That's been dedicated toward public safety and traffic and so that's one of the things we're doing," explained Commissioner Eades. "We're spending approximately $85,000 in order to put up gates both east and west on Third and on Tenth."
Costing the City instead of costing lives.
"We're doing it in order to protect the public so we never have anything like that happen again."
Construction and maintenance crews still lack about two weeks before those floodgates are up and fully functioning.


text:
By Sage Young, Hollywood Staff
Fresh off the most devastating episode of television ever, Richard Madden is back on our television screens. And this time, he's got an American accent and a hankering for the shiny stuff.
The Game of Thrones star plays prospector Bill Haskell, who finds gold, danger, and Abbie Cornish out in the Yukon in this Discovery miniseries. If this one-minute trailer truly represents the rest of the series, we are in for plenty of brooding looks, quick-draws and some very sexy old-timey PDA. Though the best part is obviously when Haskell dons a very King-in-the-North-style fur coat. Are you excited for Klondike? Let us know in the comments!
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Banda Sinaloense MS de Sergio Lizárraga - Cahuates, Pistaches
Artist Banda Sinaloense MS de Sergio LizárragaSong title Cahuates, Pistaches
Time 2:56 minYear 2009Genre(s) Latino
Label Universal Music Group - North America - Vevo
Artist Banda Sinaloense MS de Sergio LizárragaSong title Cahuates, Pistaches
Time 2:56 minYear 2009Genre(s) Latino
Label Universal Music Group - North America - Vevo
Does Anybody Want a Remake of 'I Know What You Did Last Summer'?
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Matt Damon and Paul Greengrass to Return for Fifth 'Bourne' Movie
Matt Damon is to return to play Jason Bourne in the fifth movie in the spy-thriller...
Ben Affleck Can Relate to Batman's Anger Management Issues
Ben Affleck, who will play Batman in Zack Synder's forthcoming Batman...
Want to Know How to Remove U2's 'Songs of Innocence' from iTunes?
Apple has launched a tool to help iTunes users remove U2's new album Songs...
Kiesza - Becoming Kiesza Featurette
Kiesza talks about what inspired her to become a musical artist, as well as her interest in sailing and playing the piano...
Death From Above 1979 - The Physical World Album Review
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George Clooney is Deserved Recipient of Golden Globes' Lifetime Achievement
George Clooney, the Oscar-winning actor and producer, is to be honoured with...
Robin Thicke Lied about Writing 'Blurred Lines', "I Was High and Drunk"
Robin Thicke says he was "high and drunk" during every interview he...
Can Jennifer Lawrence and Bradley Cooper Recreate Oscar Magic in 'Serena'? [Trailer]
Jennifer Lawrence and Bradley Cooper certainly appear to have a connection...
Open Grave
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Foster The People


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Starting this month, our design team will be hosting challenges here at GDS again. This will be a great way for our community to get to know our designers! Please take the opportunity to join in the challenges for this month.
We would like to thank our wonderful Legacy Ladies for hosting challenges over the past while. We really appreciate their dedication and hard work! The Legacy Ladies will be hosting some fun, new games for us each month, so make sure to look for their posts in the games and challenges thread each month. :)


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Loclly is a Buckhead-based local business built on a foundation of superior customer service and trust. We take customer service very seriously and regret anytime customer concerns are not 100% resolved in a timely manner.Though our 150,000+ Atlanta members consistently give us feedback that we are #1 in customer service in the local e-commerce industry, and our internal metrics say there is a 98% resolved rate within an average time of 64 minutes, there are times when we do not meet even our own standards. For this reason, Loclly has recently enhanced our customer service system and technology in order to continue to exceed every expectation within 2 business days of customers bringing any matter to our attention.We understand our customers have a choice when it comes to shopping online. Rest assured, Loclly's members will continue to find unbeatable deals from the top local businesses and receive first class service when and where they need it.Please feel free to contact me with any questions or for additional information about Loclly.Regards,Ainslee Marsden
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Wooden Black Board with Clip Pack of 6 Style 9076
This item is available online only. *
Mini wooden blackboards provide nostalgic signage. Whether used to create charming labels for a "Candy Bar", guest table numbers or other small messages, the hand-made quality is currently a very popular decor style. Blackboards measure: 4.5" x 3" x 3.5" H. Pack of 6
customers bought with this dress
Wooden Black Board with Clip Pack of 6 Style 9076 1 1
Cute These blackboards will be a cute touch to the wedding. I was hoping they were more wooden but they appear plastic(area around the edges). Will do its job anyhow. Good purchase for the money. November 30, 2012
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*Color & Style representation may vary by monitor. Not responsible for typographical or pictorial errors. Product availability varies by store and online. Some in-store purchases require special order and markdown styles cannot be special ordered. Contact your local store for details.


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You've heard about it in the playground, now it's in the class room. Gelli Baff turns water into goo and when you're done, like magic it changes back again.
Gelli Baff Messy Play comes in powder format in convenient bulk tubs and can be made into goo in any waterproof container, bowl or sink by simply adding the correct amount of water. Once messy play is over, the dissolver sachet turns the goo back into plain old water making it easy to clean up and it won't block or clog your drains.
Gelli Baff is a totally safe water absorbing polymer that absorbs 400 times its own weight in water, it's used in a variety of everyday products from keeping plants moist to disposable nappies. The dissolver is simple table salt, just like you put on your chips (sorry Jamie, just like you sparingly put on your veggie lasagne and side salad)
Each tub contains:
Size: L: 20cm w: 20cm H: 14.5cm