Comprehensive survey of radionuclides in contemporary smokeless tobacco products
2017

K Mcadam
H Kimpton
A Porter
C Liu
A Faizi
M Mola
J Mcaughey
B Rodu
Comprehensive survey of radionuclides in contemporary smokeless tobacco products

Chemistry Central Journal
11131201710.1186/s13065-017-0359-0RESEARCH ARTICLESmokeless tobaccoSnuffSnusRadionuclidesRadioactivityPotassium-40
There is considerable interest in the chemical composition of smokeless tobacco products (STPs), owing to health concerns associated with their use. Previous studies have documented levels of 210 Po, 210 Pb and uranium in STP samples. Here, the levels of 13 α-particle and 15 β-radiation emitting radionuclides have been measured in a broad and representative range of contemporary STPs commercially available in the United States and Sweden. For each radionuclide, the level of radioactivity and calculated mass per gram of STP are reported. The results indicate that, among 34 Swedish snus and 44 US STPs, a more complex radionuclide content exists than previously reported for these products. Of the 28 radionuclides examined, 13 were detected and quantified in one or more STPs. The most frequently identified radionuclides in these STPs were 40 K, 14 C, 210 Po and 226 Ra. Over half the STPs also contained 228 Th, and an additional 8 radionuclides were identified in a small number of STPs. The presence of 14 C, 3 H and 230 Th are reported in tobacco for the first time. The activity of β-emitters was much greater than those of α-emitters, and the β-emitter 40 K was present in the STPs with both the greatest radioactivity and mass concentrations. Since the three radionuclides included in the FDA's HPHC list were either not detected ( 235 U), identified in only three of 78 samples ( 238 U), and/or had activity levels over fifty times lower than that of 40 K ( 210 Po, 238 U), there may be a rationale for reconsidering the radionuclides currently included in the FDA HPHC list, particularly with respect to 40 K. Using a model of the physical and biological compartments which must be considered to estimate the exposure of STP users to radionuclides, we conclude that exposure from α-emitters may be minimal to STP users, but 40 K in particular may expose the oral cavities of STP users to β-radiation. Although a more comprehensive picture of the radioisotope content of STPs has emerged from this study, epidemiological evidence suggests that the levels of radionuclides measured in this study appear unlikely to present significant risks to STP users.

Introduction

There has been considerable interest in recent years in the chemical composition of smokeless tobacco products (STPs), primarily based around health concerns associated with their use. Although banned in the European Union, STPs are widely used in the United States, Sweden and Norway, and across large parts of Africa and Asia. The International Agency for Research on Cancer (IARC) has classified STPs collectively as Group 1 (known human carcinogens) [1]. However, worldwide there are very different types of STP used [1], including dry snuff (DS), moist snuff (MS), chewing tobacco (CT), hard pellets (HP) and soft pellets (SP) (predominantly in the USA), loose and pouched snus (predominately in Sweden), and a range of products used on the Indian sub-continent and in Africa. Indeed, a review of STPs by the UK Royal College of Physicians noted that different health risks are associated with the use of different STPs in line with the levels of chemical toxicants within those products [2]. In an examination of the risks associated with use of STPs [1], IARC Monograph 89 identified 28 chemical agents or toxicants that have been reported in STPs, including the radioactive elements polonium ( 210 Po) in US STPs [3] and uranium in Indian STPs [4], with the latter cited in IARC Monograph 89 as uranium-235 ( 235 U) and uranium-238 Open Access *Correspondence: firstname.lastname@example.com 1 Group Research & Development, British American Tobacco, Regents Park Road, Southampton SO15 8TL, UK Full list of author information is available at the end of the article ( 238 U) [1]. These radionuclides have subsequently been identified by the FDA as "Harmful or Potentially Harmful Constituents" (HPHC) in tobacco products and tobacco smoke [5]. A recent revision to IARC's consideration of STPs revised the summary list to 210 Po and uranium [6].

The radioactive content of tobacco, cigarette smoke and ash has been the focus of research since the early 1950s [7]. Since then a wide range of radionuclides have been identified in tobacco [8]. The 2008 report from the Scientific Committee on Emerging and Newly Identified Health Risks (SCENIHR) recognised that the radionuclide content of tobacco used for STP manufacture was important in determining the radionuclide content of STPs, and stated that radium-226 ( 226 Ra), and to some extent lead-210 ( 210 Pb), a progeny of 226 Ra, were the most important radionuclides in the tobaccos used to manufacture STPs [9]. SCENIHR also concluded that "the dose of ionising radiation from these sources must be considered as negligible in comparison e.g. with the natural radiation background and other sources of ionising radiation". Based on previous studies of the radionuclide content of tobacco and other plant materials, it seems likely that many more radionuclides are present in STPs in addition to the five listed by IARC and SCENIHR [1,9].

The main types of radionuclides that have been identified in plants arise from four distinct sources [10], three natural and one anthropogenic. The first group consists of primordial radionuclides incorporated into the planet during its formation, with half-lives comparable to the age of the earth. These include potassium-40 ( 40 K), thorium-232 ( 232 Th) and uranium-238 ( 238 U). The second group comprises the decay products or progeny of the primordial elements, which are collected into radionuclide groups known as decay series, including the 238 U series, 232 Th series and actinium series of radionuclides. The half-lives of these radionuclides cover many orders of magnitude, from thousands of years to fractions of seconds, and include 210 Pb, 210 Po and 226 Ra. The third group includes radioactive isotopes continuously produced in the earth's atmosphere by cosmic ray bombardment, such as the β-emitters: tritium ( 3 H), carbon-14 ( 14 C), and phosphorous-32 ( 32 P). The final group comprises manmade radionuclides arising in the environment principally from nuclear weapons testing and the nuclear power industry, as well as contributions from specialised (e.g. medical) uses. Examples of this group include caesium-137 ( 137 Cs), iodine-131 ( 131 I), strontium-90 ( 90 Sr) and plutonium radionuclides [11].

Environmental radionuclides enter the human body due to their ubiquitous presence in food, water, and air. Use of products containing tobacco can act as an additional exposure source since radionuclides may be present in tobacco, as in all plants, through uptake of compounds from soil, direct deposition onto leaves, or incorporation of atmospheric gases into the growing plant.

IARC has classified as carcinogenic to humans (Group 1) all radionuclides internalized within the human body that emit α-particles or β-particles for the following reasons. First, all α-particles emitted by radionuclides, irrespective of their source, produce the same pattern of secondary ionizations and the same pattern of localized damage to biological molecules, including DNA. These effects, most easily studied in vitro, include DNA double-strand breaks, chromosomal aberrations, gene mutations and cell transformation. The same is true for all β-particles. Second, all radionuclides that emit α-particles and that have been adequately studied have been shown to cause cancer in humans and in experimental animals. The same is true for β-particles including 3 H, which produces β-particles of very low energy, but for which there is nonetheless sufficient evidence of carcinogenicity in experimental animals. Third, α-particles emitted by radionuclides, irrespective of their source, have been shown to cause chromosomal aberrations in circulating lymphocytes and gene mutations in humans in vivo. Again, the same is true for β-particles: the evidence from studies in humans and experimental animals suggests that similar doses to the same tissues-for example, lung cells or bone surfaces-from β-particles emitted during the decay of different radionuclides produce the same types of non-neoplastic effects and cancers. IARC has recently also established that there is sufficient evidence in humans for the carcinogenicity of γ-radiation, and has assigned this form of radiation to Group 1, along with α-and β-emitters [12]. In addition, IARC has identified specific radionuclides as Group 1 carcinogens. There is evidence of carcinogenicity in humans for 226 Ra, 224 Ra, and 228 Ra; 232 Th and its decay products; plutonium-239 (with plutonium-240; 239,240 Pu); phosphorus-32 ( 32 P); and 131 I [11]. There is evidence of carcinogenicity in animals for many more radionuclides [12].

Given the current scientific and regulatory focus on toxicants in STPs, the paucity of studies investigating the presence of radionuclides in STPs, in comparison to the wider range of radionuclides identified in tobacco [8], highlights a significant need for thorough investigation of STP radionuclide contents. The aim of the present study therefore was to identify the levels of radionuclides in a comprehensive range of contemporary STPs, representing seven different types of product [13][14][15]. In total, 78 products representing approximately 90% of the market share for the major categories of STP in the United States and Sweden [13] were analyzed by alpha spectrometry, liquid scintillation counting and gamma spectrometry for the activity and concentration of 13 α-particle and 15 β-radiation emitters ( Table 1) broadly representative of the four major sources of radioactivity found in the environment.

Experimental section

Tobacco samples

The survey was conducted in two parts, with an initial sampling of 70 STPs from the United States and Sweden in 2008 [13], and a second sampling of 73 STPs in 2010 [14], conducted in order to ensure that the ages of the samples at the time of analysis were reflective of consumption patterns.

Details of the STP markets in the United States and Sweden were obtained in 2008, and the products for analysis were chosen to reflect approximately 90% share of these two markets at that time, including STPs from all of the principal manufacturers. A similar approach was adopted in 2010, when more than 90% of the first set of STPs were resampled, but some samples were no longer on-sale. Eight new products were sampled. These included replacements for the products no-longer sold, and examples of a new category of STP (US snus) that was not available during the 2008 exercise. In total 78 different STPs were sampled.

Both samplings included the major products in each category of STP; where there were multiple flavored variants, the base product was sampled and analyzed. In total, the survey comprised 34 Swedish products (10 L snus and 24 P snus) and 44 US products (13 CT, 5 DS, 2 HP, 1 SP, 16 MS, 6 US snus and 1 plug product) (Additional file 1: Table S1).

In both sampling exercises the products were sourced from Swedish retail websites or from retail outlets in the USA, imported into the United Kingdom, and kept frozen at − 20 °C until analysis.

Reagents

All laboratory reagents (hydrochloric acid, hydrofluoric acid, nitric acid, sulfuric acid, ferric hydroxide, copper oxide, TEA and EDTA) were from Thermo Fisher Scientific Inc. and were of Analytical Reagent Grade. Barium-133 internal tracer was supplied by Amersham International. Polonium-208, thorium-229 and plutonium-242 internal tracers were supplied by the National Physical Laboratory (UK). Uranium-232 internal tracer was supplied by Harwell Technology (Oxford, UK).

Measurement of water content in the STP samples

To convert measurements made on a wet-weight basis (wwb) to a dry-weight basis (dwb), the water content of all STPs was measured by near-infrared (NIR) spectroscopy using a standard technique wherein water was extracted from the STPs using dry methanol. A calibrated double-beam spectrometer was used to measure the intensity of the combination band at 1943 nm (due to -OH stretching and H-OH bending of the water molecule); intensities were compared to standards containing water in methanol for the purposes of quantification.

Measurement of ash content of STPs

The inorganic material content of STPs was estimated by heating the STP at 500-550 °C in air in a pre-dried silica dish placed in a muffle furnace for 1 h. Organic material present in the sample during this time period was burnt off as combustion gases; if the resulting ash was not uniformly white (the presence of carbon particles in the ash indicates incomplete ashing of the STP) then the samples were ashed for a further 30 min. The residual sample weight after ashing, with allowance for the original moisture content of the STP, provided an estimate of the STP's inorganic content.

Determination of radionuclides

The radionuclides examined in the present study are listed in Table 1. Also summarized in the table are their sources, main radioactive decay modes, measurement methods in this study, half-lives and specific activities. All radionuclide analyses were conducted by Environmental Scientifics Group (Didcot, UK), from whom further method details can be obtained. Po was determined by wet oxidation. 208 Po was added to the sample as an internal tracer. A nitric acid/hydrofluoric acid mixture was added to an aliquot of the homogenized sample and then taken to dryness. This was repeated, then nitric acid was added and the sample taken to dryness to remove any traces of hydrofluoric acid. The residue was dissolved in hydrochloric acid, and polonium was isolated by auto deposition onto a silver disc (Fourjay Limited, UK) under reducing conditions. The radioactivity on the silver disc was measured by alpha spectrometry to determine the ratio of 210 Po to 208 Po.

226

Ra

Levels of 226 Ra were determined by adding a known activity of 133 Ba tracer to a dried and ground aliquot of the sample, which was then ashed in a furnace overnight. The sample was then digested in aqua regia (3:1 mix of hydrochloric:nitric acid). The radium radionuclides were initially co-precipitated with lead and barium sulfates from a faintly acidic water sample. The precipitate was isolated by centrifuging, then redissolved in an alkaline solution of ethylenediaminetetraacetic acid (EDTA) and triethanolamine (TEA). The radium radionuclides were then co-precipitated with barium sulphate from acetic acid medium free of lead contamination. The barium/ radium sulphate was then further purified by a series of precipitations and finally mounted as a thin source on a 5 cm diameter stainless-steel planchet. Chemical recovery was determined by measurement of 133 Ba by γ-ray spectrometry (High Purity Germanium Detector and NIM electronics, EG&G Ortec, AMETEK, Inc). After a 21-day ingrowth period, the source was counted for gross α-activity on a Berthold LB770 low-level proportional counter (LB 770 10-Channel α-β-low-level counter, Berthold Technologies GmbH & Co.) for 1000 min. This determines the α-activity of 226 Ra and its daughters in secular equilibrium ( 222 Rn, 218 Po and 214 Po). The 226 Ra activity was given by dividing the gross α-activity by four.

Thorium isotopes ( 232 Th, 230 Th, 228 Th)

An aliquot of the homogenized sample was spiked with a 229 Th internal standard and then ashed at 450 °C. The ashed residue was dissolved in hydrofluoric acid. Thorium was concentrated by co-precipitation with ferric hydroxide. Following dissolution of the precipitate using nitric acid, the thorium was purified using ion-exchange chromatography (disposable plastic columns with Analytical Grade ion exchange resin, Eichrom Technologies, Inc.). The purified thorium was electrodeposited onto a stainless-steel disc (Fourjay Limited, UK), thorium activity was measured by α-spectrometry (Octéte, EG&G Ortec, AME-TEK, Inc. and Alpha Analyst, Canberra UK Limited).

234

U, 235 U and 238 U

Uranium-232 internal yield tracer was added to a dried and ground aliquot of the sample and ashed in a furnace overnight. The ashed residue was dissolved in hydrochloric acid following pre-treatment with hydrofluoric and nitric acids. After co-precipitation of the uranium with ferric hydroxide, ion-exchange chromatography (disposable plastic columns with Analytical Grade ion exchange resin, Eichrom Technologies, Inc.) was used to further purify and separate the uranium, which was then electrodeposited onto stainless-steel discs (Fourjay Limited, UK). Measurement of the uranium isotopes was carried out by alpha-spectrometry.

238

Pu, 239,240 Pu

Plutonium-242 yield tracer was added to a dried and ground aliquot of the sample and ashed in a furnace overnight. The sample was then digested in aqua regia. After co-precipitation of the nuclides of interest with ferric hydroxide, ion-exchange chromatography (disposable plastic columns with Analytical Grade ion exchange resin, Eichrom Technologies, Inc) was used to further purify and separate the plutonium from americium. The plutonium was then electrodeposited onto stainless-steel discs. Measurement of the plutonium isotopes was carried out by alpha-spectrometry.

H

A sub-sample of known weight was taken from each sample and then burnt in an oxygen rich atmosphere in the presence of a copper oxide catalyst. Under these conditions, the hydrogen species were converted to water vapor, which was then selectively trapped in a series of gas-bubblers containing 0.1 M nitric acid. Aliquots of known weight of this liquid were then assessed for their tritium content by liquid scintillation counting (1220 QUANTULUS Ultra Low Level Liquid Scintillation Spectrometer, PerkinElmer Inc.). The tritium activity was corrected for the proportion of the bubbler trapping solution taken and for the weight of sample combusted to yield the specific activity in the sample.

14

C

A sub-sample of known weight was taken from each sample and then burnt in an oxygen rich atmosphere in the presence of a copper oxide catalyst. Under these conditions, the carbon species were converted to carbon dioxide. This was then selectively trapped in a series of gas-bubblers containing a trapping medium. Aliquots of known weight were then assessed for their carbon-14 content by liquid scintillation counting (1220 QUANTU-LUS Ultra Low Level Liquid Scintillation Spectrometer, PerkinElmer Inc.). The carbon-14 activity was corrected for the proportion of the bubbler trapping solution taken and for the weight of sample combusted.

Gamma spectrometry

Gamma ray spectrometry was used to measure the activity of 40  The measurement technique was based on the use of high-purity germanium (HPGe) detectors coupled to the required pulse amplification and shaping electronics and multi-channel analyser (EG&G Ortec, AMETEK Inc.). The γ-ray spectra were stored on a computer and analysed via the software program FitzPeaks Gamma Analysis and Calibration Software (JF Computing Services) for photopeak identification and quantification. The detectors were calibrated for efficiency, energy and peak shape using a certified mixed radionuclide standard, which covers an energy range of approximately 30-2000 keV. The efficiency of γ-rays between 30 and 120 keV was determined on an individual basis. Application of decay corrections for the naturally occurring daughter radionuclides of uranium and thorium assumes that the series daughter radionuclides are all in secular equilibrium and therefore decay with the half-life of the first radionuclide in the series.

Instrument calibration

All instruments are calibrated using certified standards traceable to national standards. The radioactive controls and internal tracers are also made from certified standards and are supplied by various manufacturers: NPL (UK), Amersham International and National Institute of Standards and Technology (NIST, USA).

Limit of detection (LoD)

The LoDs were calculated in accordance with International Standard ISO 11929-7. The generic formulae for the detection limit can be simplified by setting a value for the coverage factor (chosen to be 1.645 for 95% probability), and by assuming that the count time is the same as the background count time and that there is negligible relative error in w (u rel (w)). The formula for the limit of detection (LoD) in Bq/L or Bq/kg is:

Where the symbols are defined as follows: b = background count rate (counts/s) (includes continuum when sample present and background when no sample present), t s = sample count time (s), w = 1/(e V f ) or 1/(e M f ), u rel (w) = total relative standard uncertainties for all the factors making up w.

When calculating the limits of detection in gamma ray spectrometry, it is important to take into account the increased uncertainty from estimating the continuum from a smaller number of channels when peaks are located close together. This is therefore incorporated into the recommended formula above for the peak integration case as follows and in a re-arranged format:
LoD = 2.7w t s + 4.7w b t s
Additional symbols used: n = peak width in channels, m = number of channels used each side of the peak to determine the continuum. Where n 2m is usually about 1. However if gamma ray peaks are close together and the number of channels available for continuum estimation is reduced then n 2m could increase to possibly 4 or more.

A single measurement on each sample was made and a full uncertainty budget calculated as described in the Measurement Good Practice Guide No. 36, British Measurement and Testing Association. The uncertainty is quoted at the 95% confidence level.

General comments on LoD

Different LoDs were calculated for different samples of the same analyte; these arise from the factors used in the calculation for the limit of detection in the formula shown above. The values of some factors, such as b, differed from measurement to measurement, resulting in different LoDs for many samples.

The background for most techniques is fairly constant, but this is not the case for analysis by gamma ray spectrometry. Here the individual sample background is the Compton continuum produced by the gamma rays in the spectrum. If, for example, the K-40 level is low in one sample, the Compton continuum will be low and therefore the background will be low. Conversely if the K-40 activity is high, the Compton continuum will be higher and therefore the background will be higher.

Data presentation and analysis

Measured values for radionuclides in STPs were obtained as measurements of the radioactivity of the sample as received (or wet weight basis, wwb). Values are reported both as activities (mBq/g) and corresponding mass concentrations (g/g) calculated from the specific activities (SA) given in Table 1; the data are presented per gram because STP users commonly use quantities of approximately 1 g or more of snus per application [16]. Mass concentrations allow direct comparison of the data reported here with levels of other chemical toxicants in tobacco. The data are also given on a dry weight basis (dwb), i.e. after the sample weight is adjusted for the water content, as measured by NIR (Additional file 1: Table S1).

The wwb values reflect the radionuclide content of the STP as experienced by the user (and measured in this study), whereas the dwb values refer to the radionuclide content of the solid matter of the STP (predominately tobacco) and is reported here to facilitate a comparison
LoD =   2.71 + 3.29 � � 1 + n 2m � × B T   × w
both across different types of STP and with published values, which are predominantly reported historically as dwb. Activity data that were originally reported in the literature in units of pCi/g have been converted to mBq/g. Half-lives (τ), SAs and % isotopic compositions were taken from references [17,18]. Radionuclide levels across categories of different STPs were compared using the General Linear Model ANOVA in Minitab v16. Where reported activity levels were below limits of quantification (LOQ), randomly imputed values between the LOQ and zero (generated using Microsoft Excel 2010) were used for the purpose of these comparisons.

Results

Although only 210 Pb, 210 Po and uranium have been previously reported in STPs, many other radionuclides have been reported to be present in the tobacco plant and tobacco products [8]. The activities of the 28 radionuclides measured in contemporary Swedish snus and US STPs on a wwb are summarised in Tables 2, 3

Uranium-235 and radionuclides of the uranium-238 decay series

The activity values of uranium-235 and radionuclides of the uranium-238 decay series are presented in Additional file 1: Table S2, and the corresponding mass concentrations in Additional file 1: Table S5.

Uranium-238 ( 238 U, 99.27% of naturally occurring uranium) is a primordial isotope that gives rise to the uranium decay series including uranium-234 ( 234 U, 0.0054% of naturally occurring uranium). Uranium-235 ( 235 U, 0.72% of naturally occurring uranium) is also a naturally occurring isotope, but is part of the actinium series. In the current work these three radionuclides are discussed together because of the way in which uranium levels have been historically reported, sometimes as total uranium and sometimes as the individual radionuclides.

In the present study 238 U was detected in only three samples (2 HP, 1 MS) at an activity of 0.8−9.9 mBq/g wwb, 234 U was detected in 5 products (2 HP, 2 MS, 1 portion snus) at an activity owf 0.96-8.8 mBq/g wwb, and 235 U was not detected in any of the STP samples analyzed ( Table 2). In the samples where both 238 U and 234 U were present, the two radionuclides had very similar activities; however, owing to the greater specific activity of 234 U, a substantially greater mass   None reported concentration of 238 U (6.5−80.0 × 10 −8 g/g wwb) was detected as compared with 234 U (4-38 × 10 −12 g/g wwb) (Additional file 1: Table S5).

Referring to Table 2, mass concentrations of total uranium have been reported in a variety of cigarettes and chewing tobaccos from the Netherlands and India in the range 8-240 × 10 −9 g/g, compared with 7.4-19.1 × 10 −6 g/g in Indian snuff products. The higher levels in Indian snuff was suggested to be due to the inclusion of wood ash/calcium hydroxide [4,19]. For the individual isotopes 234 U, 238 U our results are of the same order of magnitude but slightly higher than those reported for Brazilian and Egyptian tobaccos [20,21]. Given that the majority of the samples measured in the current study did not have measurable levels of uranium radionuclides, it is of value to estimate the upper limits for their presence in these STPs based on the current analytical capabilities. For the current samples with no measurable uranium radionuclides, the sample dependent upper limits (g/g wwb) were < 3.2−25 × 10 −8 for 238 U, < 3-13 × 10 −12 for 234 U and < 4−25 × 10 −9 for 235 U.

Although there have been two previous reports of thorium-234 ( 234 Th) in tobacco, it was not detected in any of the STPs we analysed ( Table 2). But because other members of the uranium decay series were identified in all samples, 234 Th is likely to be present in the STPs albeit at levels below the reporting limits of the analysis (< 20-40 mBq/g wwb, corresponding to < 2.3-4.7 × 10 −17 g/g wwb).

Protactinium-234 ( 234 Pa) has not previously been reported in tobacco products, and none of the STPs we analysed had measurable levels of 234 Pa ( Table 2). The upper limits for activity and mass concentration of 234 Pa were estimated as < 200-600 mBq/g wwb and < 2.7-8.1 × 10 −18 g/g wwb respectively.

Thorium-230 ( 230 Th) has not previously been reported in tobacco. In the present study, five STPs (1 CT, 2 HP, 2 MS) had measurable 230 Th levels, with an activity of 1-9.2 mBq/g wwb (Table 2), and a mass concentration of 1.0-12.1 × 10 −12 g/g wwb (Additional file 1: Table S5). Similar to 234 Th, however, the STPs containing other members of the 238 U decay series are likely to contain 230 Th at levels below the reporting limits of the analysis, calculated as < 1-8 × 10 −12 g/g wwb.

Radium-226 ( 226 Ra) was identified in all but three of the samples at an activity of 0.4−8.8 mBq/g wwb (0.5−17.6 mBq/g dwb) ( Table 2), corresponding to a mass concentration of 1.1 to 24 × 10 −14 g/g wwb (1.4−48 × 10 −14 g/g dwb) (Additional file 1: Table S5). ANOVA based comparison of product categories on a wwb showed that 226 Ra content was similar among most STP categories, except for HP and DS products which had significantly higher levels than MS or CT. On a dryweight basis, there were generally similar 226 Ra contents among the STPs analyzed, except that loose and pouched snus had higher levels than CT. Referring to Table 2, several studies have reported 226 Ra levels in cigarette, pipe and cigar tobaccos from various geographic sources covering a range of 1.8-20 mBq/g dwb, which are comparable to the values found in the present study.

Although lead-214 ( 214 Pb) and bismuth-214 ( 214 Bi) have previously been reported in tobacco, none of the STPs analyzed showed any 214 Pb or 214 Bi ( Table 2). The upper limits of activity in the STPs were calculated as < 3-8 and < 3-9 mBq/g wwb respectively, corresponding to a maximum possible content of < 2.5-6.6 × 10 −21 and < 1.8-5.5 × 10 −21 g/g wwb respectively.

Among the naturally-occurring radionuclides that become incorporated into tobacco plants, polonium-210 ( 210 Po) has received the greatest attention of any radionuclide due to its transfer to smoke in cigarettes [22], and potential for causing lung cancer [23]. In the present study, 210 Po was detected in 66 of the samples analyzed ( Table 2). The measured activities ranged from 1.8−18 mBq/g wwb (3.2−21 mBq/g dwb), corresponding to a mass concentration of 1.1−11 × 10 −17 g/g wwb (2−13 × 10 −17 g/g dwb) (Additional file 1: Table S5). Two snus portion products and 2 CT products evaluated in our study were below the detection levels. Comparing the different STP categories the SP and DS products had higher 210 Po activities (wwb) than the other categories. The 210 Po content of both loose and pouched snus was lower than the other product categories except for CT. On a dry-weight basis, the DS, MS and SP products had higher 210 Po activities than the portion, loose snus and CT products. Our results for 210 Po activities in DS (11.0-17.0 mBq/g wwb) and MS (6.2-9.4 mBq/g wwb) are consistent with previously reported values for these STPs (Table 2).

In the present study, 210 Pb activities were below the limit of detection of the assay (< 40 mBq/g) for all samples examined. Historic data for 210 Pb contents of tobacco products are consistent with this ( Table 2). A number of authors have reported secular equilibrium between 210 Pb and 210 Po due to the length of time between harvesting of tobacco leaves and tobacco product production [24][25][26][27]. Consequently, 210 Pb is likely to be present in the current sample set, at activity levels comparable to the 210 Po measurements.

Radionuclides of the thorium-232 decay series

The activity values for thorium-232 decay series radionuclides are presented in Additional file 1: Table S3, and the corresponding mass concentrations in Additional file 1: Table S6.

The primordial isotope thorium-232 ( 232 Th) accounts for virtually 100% of the natural abundance of thorium. In the present study, only one US CT product showed a detectable level of 232 Th (1.1 mBq/g wwb), representing a mass concentration of 0.27 × 10 −6 g/g wwb (Additional file 1: Table S6). The corresponding dwb values (1.5 mBq/g and 0.37 × 10 −6 g/g) are in line with levels reported in the literature for total thorium levels ( Table 3).

Although the isotope actinium-228 ( 228 Ac) is extremely rare (almost all naturally occurring actinium is 227 Ac), it has been reported in several tobacco samples (Table 3). However, 228 Ac was not detected in any of the current STPs (Table 3). From the reporting limits, the maximum activity and mass of 228 Ac that could be present in the STPs were < 8−30 mBq/g wwb (< 16−62 mBq/g dwb) and < 1−3.6 × 10 −19 g wwb (< 2−7.5 × 10 −19 g dwb), respectively. The current method is insensitive to the levels of 228 Ac reported historically of 0.4-6.5 mBq/g ( Table 3).

In the current work, 47 of the STPs had detectable levels of 228 Th with activity ranging from 1.3 to 8.5 mBq/g wwb (2.2−15.8 mBq/g dwb) (Table 3), corresponding to mass concentrations of 4.3-28.0 × 10 −17 g/g wwb (7.4−52.0 × 10 −17 g/g dwb) (Additional file 1: Table S6), with many of the measured activities similar in magnitude to the limit of quantification of the analysis. These values are at the lower end of those reported in the literature (Table 3). ANOVA analysis of wwb data showed that DS had higher 228 Th activity levels than MS products. When expressed as a dry weight basis, there were no significant differences among the product categories.

None of the STPs tested had measurable levels of lead-212 ( 212 Pb), bismuth-212 ( 212 Bi) or tantalum-208 ( 208 Tl) ( Table 3). From the reporting limits of Fig. 1 Visual comparison of typical Swedish portion snus (left) and Oomph portion snus (right). Shown are cross-sections of the cut products the analytical methods, the maximum levels of these nuclides in the STPs were, respectively, < 2−6 mBq/g wwb, < 20−60 mBq/g wwb and < 2−5 mBq/g wwb in terms of activity; and < 3.9−12 × 10 −20 g/g wwb, < 3.7−11.1 × 10 −20 g/g wwb and < 1.8−4.6 × 10 −22 g/g wwb. Although not detected here, trace levels of 212 Pb and 208 Tl have been reported in Swiss cigarettes [28], and 212 Pb and 212 Bi levels have been quantified [29] in Iraqi cigarettes at 6-9 and 9.9-17 mBq/g, respectively (Table 3).

Other naturally occurring radionuclides-potassium-40, tritium, carbon-14

For these naturally occuring radionuclides the activity values in the analysed STPs are presented in Additional file 1: Table S3, and the corresponding mass concentrations in Additional file 1: Table S6.

Potassium-40 ( 40 K), present at 0.012% of naturally occurring potassium, was identified in all but one of the STPs analyzed ( Table 3). The activity levels of 390-1900 mBq/g wwb (419-2145 mBq/g dwb) make it the most radioactive component present in the measured STPs. Furthermore, 40 K was the radionuclide present at the highest mass concentrations, 1.5-7.2 × 10 −6 g/g wwb (1.6-8.1 × 10 −6 g/g dwb); an order of magnitude higher than the next most prevalent radionuclide (Additional file 1: Table S6). These data are within the range of values reported in the literature (49-2183 mBq/g) ( Table 3).

Comparing product categories on a wwb showed higher activity levels for DS products, with all other products having similar or lower levels of activity. On a dwb the differences between STP categories diminished, although DS products were still at the higher end of 40 K content. Activity levels were also lower in CT than in DS and MS products. The sample with no measurable 40 K content, Oomph (Wise) citrus and menthol (Northerner), is a very dry product with an upper limit of < 0.4 × 10 −6 g/g 40 K. Compared to the other snus products the material in the Oomph pouch was lighter in colour and contained a substantial content of a white material (Fig. 1). This was probably due to the cellulose powder and vegetable fibre ingredients reported on the package. The tobacco content comprised 50% of the total product mass. Hence, the lack of detectable 40 K may well reflect the diluted tobacco content of this STP.

Carbon-14 ( 14 C) is largely a product of cosmic ray irradiation of the atmosphere. The 14 C content of tobaccos has not been reported previously, although an assimilation study [30] has shown that 14 C is readily taken-up and distributed within the tobacco plant. In the current work, 14 C was detected in all but one of the STPs, making it one of the more pervasive radionuclides examined in this study. 14 C activity ranged from 20 to 101 mBq/g wwb (26-135 mBq/g dwb) (Table 3), with a corresponding mass concentration of 1.2 to 5.9 × 10 −13 g wwb (1.5-8.0 × 10 −13 g/g dwb) (Additional file 1: Table S6). The product without measurable 14 C was Romeo y Julieta (Habanos Nordics), a portion snus; for this product, the detection limit was higher than for many of the other STPs; thus, 14 C may have been present at a level slightly below the limit of detection. Comparison of 14 C activity levels across different product categories showed no significant differences on either a dwb or wwb.

Although tritium ( 3 H) which is also produced by cosmic ray interaction with the atmosphere, has not been reported in tobacco, it was considered a potential contaminant via generation in the atmosphere, and incorporation into the growing tobacco plant as 3 H-incorporated water. 3 H was identified in one Swedish snus and one US plug product at up to 65 mBq/g wwb (79 mBq/g dwb) ( Table 3) or 1.8 × 10 −16 g/g wwb (2.2 × 10 −16 g/g dwb) (Additional file 1: Table S6). The other samples had upper limits for 3 H activity and content of < 10-43 mBq/g wwb and < 2.8-12 × 10 −17 g/g wwb, respectively.

Phosphorus-32 ( 32 P) is another radionuclide generated by cosmic ray interaction with the atmosphere, and has been categorized by IARC as a Group 1 carcinogen. However, its short half-life (14.3 days) and absence of precursors in the STPs would make its presence in these samples highly unlikely, and was therefore not assayed in the present study.

Man-made radionuclides

The activity values for man-made radionuclides in the analysed STPs are presented in Additional file 1: Table  S4, and the corresponding mass concentrations in Additional file 1: Table S7.

The synthetic radionuclide americium-241 ( 241 Am) is generated within nuclear waste. Its presence in tobacco has not been reported previously and it was not found in any of the present STPs analyzed (Table 4). Using the activity reporting limits of < 2-4 mBq/g wwb as upper limits of its potential presence in the STPs, indicated a maximum possible mass concentration of < 1.6-3.2 × 10 −14 g/g wwb (Additional file 1: Table S7).

The STPs were analyzed for three plutonium radionuclides, 238 Pu, 239 Pu and 240 Pu, which are products of the nuclear reactions of uranium. In the present study, four samples (2 portion snus, 1 CT and 1 MS) had measurable 238 Pu activities (0.4-1.1 mBq/g wwb; 0.6-2.3 mBq/g dwb) ( Table 4) corresponding to 6.9-16.9 × 10 −16 g/g wwb (8.9-37 × 10 −16 g/g dwb) (Additional file 1: Table  S7). For the samples with no measurable 238 Pu, the upper limits of activity were estimated as < 0.06-0.7 mBq/g wwb, corresponding to mass concentrations of < 0.95-11 × 10 −16 g/g wwb. Seven of the STPs (2 portion snus, 3 CT, 2 MS) showed measurable 239,240 Pu contents with activities of 0.3-1.3 mBq/g wwb (0.6-2.9 mBq/g dwb).

For the STPs with no measurable 239,240 Pu, the upper limits of activity were < 0.06-0.7 mBq/g wwb. The activities of 239,240 Pu were converted to masses by assuming that 239 Pu comprises 80% of the total amount of 239,240 Pu present, which is a balance between the value of approximately 95% 239 Pu found in weapons-grade plutonium, 84% identified in global atmospheric fall-out and approximately 70-75% 239 Pu found in reactor-grade plutonium [31]. These values correspond to 7.3-30 × 10 −14 g/g wwb (14-65 × 10 −14 g/g dwb) of 239 Pu and 1.8-7.5 10 −14 g/g wwb (3.5-16.3 × 10 −14 g/g dwb) 240 Pu.

Among the STPs found to contain plutonium in the present study, the activity levels of 239,240 Pu were considerably higher than those reported [32] for Finnish cigarette tobaccos in the 1960s (0.396 mBq/g), 1970s (0.012 mBq/g) and 1980s (0.005 mBq/g), which probably reflects different geographic sources of tobacco between the studies.

Both caesium-137 ( 137 Cs) and caesium-134 ( 134 Cs) are products of nuclear fission reactions, and are contaminants produced in nuclear incidents. In the present study, neither 137 Cs nor 134 Cs was detected in any of the STPs examined (Table 4). The upper limits for the possible presence of 137 Cs and 134 Cs in the STPs were estimated as < 2-4 mBq/g wwb 137 Cs and < 2-5 mBq/g wwb 134 Cs, corresponding to mass concentrations of < 6.2-12.4 × 10 −16 and < 4.2-10.4 × 10 −17 g/g wwb, respectively. Both 134 Cs (up to 10 mBq/g) and 137 Cs (0.1-40 mBq/g) have been reported previously in various tobaccos (Table 4). It has been suggested that geographic source is a determinant of its presence or absence in tobacco [28].

Iodine-131 ( 131 I) co-evolves with caesium radionuclides after nuclear reactor incidents. It has not been reported previously in tobacco, probably due to its very short halflife, and it was not detected in any of the current STPs (Table 4).

Cobalt-60 ( 60 Co), which is also a product of nuclear fission, was not detected in any of the STPs. From the reporting limits of the method, the maximum possible activity was < 3-10 mBq/g wwb for 131 I and < 2-6 mBq/g wwb for 60 Co, corresponding to a mass concentration of < 6.5-21.7 × 10 −19 g/g wwb and < 5-14 × 10 −17 g/g wwb, respectively. It has not been reported previously as a natural contaminant in tobacco but has been detected in neutron activated tobacco in laboratory studies [33].

Discussion

The present study represents the most comprehensive assessment of the radionuclide content of STPs published to date. Seventy-eight contemporary STPs from the USA and Sweden, covering the main product categories and manufacturers, were assessed for the presence of 28 radionuclides, encompassing all of the major sources of environmental radioactivity. Three of the species for which we found quantifiable amounts ( 14 C, 3 H, and 230 Th) have not previously been reported in tobacco.

Several radionuclides are present at low levels in STPs

In contrast to the conclusions of recent literature reviews of radionuclides in STPs [1,6] focusing on 210 Po, 235 U and 238 U, this study has revealed a plurality of radionuclides in contemporary STPs. All STPs were found to contain α-and β-emitting radionuclides [generically categorized by IARC as Group 1 carcinogens when internally deposited- Table 1], and the specific IARC Group 1 carcinogens 226 Ra, and 232 Th were identified in a number of STPs. However, none of the radionuclides investigated were detected in all STPs. 14 C, 226 Ra, 210 Po (and by inference, 210 Pb) and 40 K were found in almost all (66)(67)(68)(69) of the STPs examined, 228 Th, was identified in over half, and 3 H, 238 Pu, 239,240 Pu, 238 U, 234 U, 232 Th, and 230 Th, were found in only a few. Other than 40 K, the mass of radionuclides measured in these STPs were very low in comparison with other toxicants identified in STPs [1,6], often by many orders of magnitude.

Members of both the 238 U and 232 Th decay series were present in the STPs. The most active species from the 238 U series were 210 Po ( 210 Pb) > ( 238 U ~ 234 U ~ 230 Th ~ 226 Ra) respectively in order of activity. For the 232 Th series, only 232 Th and 228 Th were detected, with 228 Th showing greater activity. Radium-228 ( 228 Ra; τ, 5.74 years; SA = 10.1 TBq/g) is a member of the 232 Th series that was not examined in this study; however, previous reports suggest that it might be present in tobacco at levels similar to or slightly higher than those of the other members of the 232 Th decay series [34][35][36].

Radionuclides resulting from cosmic ray irradiation of the atmosphere were also found in the STPs. Particularly notable is the presence of the β-emitter 14 C, which was found in all but one of the samples examined; 14 C has not been reported in tobacco before, and it represents a previously unconsidered source of radioactive exposure from tobacco products. 3 H, also not reported previously in tobacco products, was identified in two STPs. In these two samples, although 3 H was present at much lower mass concentrations than 14 C, its radioactivity levels were similar to 14 C. The substantially lower mass concentrations of 3 H than 14 C probably reflect differences in atmospheric production rates and subsequent uptake by the growing tobacco plant. Among the man-made radionuclides examined, some STPs showed measurable quantities of three plutonium radionuclides.

Many radionuclides are either undetectable or absent from STPs

Although some members of the 238 U and 232 Th decay series were present, others ( 234 Th, 234 Pa, 214 Bi, 214 Pb, and   228 Ac, 212 Pb, 212 Bi, 208 Tl respectively), as well as 235 U, 131 I and the two caesium radionuclides, showed no activity in any of the STPs examined. Some of these radionuclides have been previously detected in tobacco ( 228 Ac, 214 Bi, 134 Cs, 137 Cs, 214 Pb, 212 Pb, and 235 U). When a species was not detected it may be due either to the absence of the species in the analyzed matrix or to insufficient sensitivity of the analytical method for the sample being examined.

There are some indications to the reasons underlying the absence of measured activity from specific radionuclides in some samples. The presence of members of the 238 U and 232 Th decay series, particularly the originating radionuclides, in an STP means the presence of other members of the decay series cannot be precluded, albeit at levels below the detection limit of the assay. This is exemplified by the uranium isotopes examined in this study. No STP was found with measurable 235 U, five samples showed detectable levels of both 234 U and 238 U, and two STP samples were found to contain 234 U but did not have measurable levels of 238 U. Natural sources of uranium contain these radionuclides at a ratio of 99.27% 238 U to 0.72% 235 U to 0.0054% 234 U; however, 234 U is the most radioactive uranium isotope, and thus lower concentrations could be detected by the method used in this work. Therefore, 238 U and 235 U will also be present, even if not detectable, in the samples containing 234 U. Moreover, given the very short half-lives of many of the progeny of the 238 U decay series (such as 214 Pb and 214 Bi) it is reasonable to assume that such species may be present, however fleetingly, at some point between production and consumption of an STP.

In contrast, some of the man-made radionuclides with relatively short half-lives (e.g. 137 Cs, 134 Cs, 131 I) were not detected in the STPs, and it is plausible that these species are not present owing to a combination of their decay rates and the age of the tobacco in the STPs post-harvest. The radionuclides 134 Cs (τ = 2 years) and 131 I (τ = 8 days) would be expected to have decayed to their progeny in the time scale between recent nuclear reactor incidents (e.g. Chernobyl in 1986) and the date of this study (2008)(2009)(2010). However, 137 Cs (τ = 30 years) would have undergone less decay since its emission into the environment following the Chernobyl nuclear accident; therefore, the absence of detectable 137 Cs probably reflects low levels, if any, absorbed from the environment into the tobaccos used to make these STPs. The analytical method is sufficiently sensitive to detect the levels reported in many of the historical observations, and therefore 137 Cs may not be present in these STPs. The plutonium radionuclides identified in small numbers of STPs during the present work have half-lives from 87 to 24,000 years. Appreciable quantities of plutonium radionuclides were released into the atmosphere during atmospheric nuclear weapons testing in the mid to last half of the 20th century, and their presence has subsequently been detected in several plant species [32]. However, 241 Am (τ = 432 years), also a product of man-made nuclear reactions, and a daughter product of 241 Pu, was not detected in the STPs, but may be present at levels below the sensitivity of the method.

In the present work, upper bounds for the possible presence of undetected radionuclides were calculated from the reporting limits of the activity counting method. For some radionuclides with very short half-lives, the upper reporting limit corresponds to a few atoms of the radionuclide within the STP sample. Notably, no radionuclides were detected with half-lives shorter than 132 days. Conversely, all naturally present radionuclides (other than 235 U, which, if present in these STPs, would have levels below the sensitivity of the analytical method) with half-lives greater than 132 days were detected in some of the STPs examined in this work. This may either point to an effective cut-off point, based on radionuclide half-life, for the analytical capability of the current approach, or perhaps reflect the age of tobacco at the time of measurement.

Activity from β-emitters in STPs by far exceed those of α-emitters

The 2008 SCENIHR report [9] stated that "according to Hoffmann et al. [37], the average total activity of alpha emitters in 5 major brands of US snuff was found to be ß activity α activity Fig. 2 Pie-chart comparing mean α and β activities from STPs 0.16-1.22 pCi/g" (6-45 mBq/g). Examination of the Hoffmann et al. study [37] reveals the SCENIHR reports statement to be incorrect, and probably an underestimate, in that Hoffmann et al. reported the presence of 0.16-1.22 pCi/g 210 Po, rather than total α-activity, in 5 US snuff brands. The total α-emissions from the STPs in the current study ranged from 4 to 50 mBq/g wwb and β-emissions ranged from 164 to 1980 mBq/g wwb (plus the unmeasured contribution of 210 Pb, estimated by comparison to 210 Po at 1.8-18 mBq/g). Mean values for total  α-and total β-emissions are graphically compared in Fig. 2, which clearly shows that total β-emissions are substantially greater than total α-emissions, with β-emissions accounting for 98% on average of the measured activity. Figure 3 shows that in terms of the radioactive emissions from constituents within STPs, the greatest contribution by far was from the β-emitter 40 K; and, when detectable, the activities of the other β-emitters ( 14 C, and 3 H) were also greater than that of α-emitters. Unlike the potential risk from more volatile radionuclides such as 210 Po in cigarette tobacco, transfer to smoke is not a factor in assessing exposure to radionuclides in STPs. Among the STPs examined here, the radioactivity of 210 Po was approximately 1% of that of 40 K, and therefore 210 Po is a relatively minor contributor to STP radioactivity. Although as depicted in Fig. 4, and discussed later in more detail, the presence of a given radionuclide in an STP cannot be directly extrapolated to human exposure.

Radionuclide content varies with STP product type

Some variations in radionuclide content were observed among the different STP categories. Only the HP products had consistently measurable levels of 238 U, 234 U and 230 Th. HP products also had higher levels of 226 Ra than the other categories on a wwb. The higher levels of these radionuclides likely reflect the presence of non-tobacco (such as calcium carbonate [38]) materials within the HP products. Estimation of the inorganic content (via ashing) of the STPs showed higher inorganic contents in the HP products than in CT, MS, plug, SP, loose snus and all pouched snus other than the low moisture brands. However, the inorganic contents of the DS, dry pouched snus and HP products were comparable. Hence these measurements suggest that the nature of the non-tobacco materials in the HP products may be more important than the quantity. Uranium is known to interchange with calcium in bone samples [39], and the presence of calcium salts in the HP products may act as a source of uranium and daughter radionuclides in STPs.

For the most abundant radionuclide present, 40 K, the highest levels were found in DS products, and the lowest in an STP whose tobacco content appeared diluted with other materials. No differences were found among product categories for 14 C or 228 Th when adjusted for the moisture content of the STPs. The STPs in which 238 Pu and 239,240 Pu were detected had similar levels of these man-made radionuclides.

A review of the literature generally indicates that the radionuclides we identified in STPs are similar to levels historically reported in tobacco, except, as noted above, where non-tobacco materials appear to be included in the STP. However, we identified several radionuclides in STPs that have not previously been reported in tobacco.

Assessing exposure to radionuclides in STPs There is no existing radiological model for evaluating exposure from STPs

Establishing the radionuclide content of STPs is an essential first step in understanding the incremental contribution of radionuclides associated with STP use to the background exposure from radionuclides in our diet, water and air. A key step is to calculate the radiation dose to tissues of STP users, because it allows estimation of the relative risk profiles of different STP product categories, and in principle it facilitates estimation of the risks associated with radionuclides in STPs. Models exist for calculating the radiation dose (exposure energy divided by mass of exposed tissue) resulting from exposure to radionuclides present in our diet, water and air, as well as from occupational exposure (e.g. [40][41][42]).

However, the type of exposure associated with STP use (shown schematically for use of a generic STP in Fig. 4) is somewhat different to established exposure models. Perhaps the closest established model is that used to calculate the exposure to, and risk from, ingested radionuclides. However, models of ingestion assume rapid mouth transit of the ingested material, and also incorporate the metabolic processes of the body that lead to dispersal of the radionuclide from the gastrointestinal tract to the physiologically preferred accumulation site (e.g. the skeleton for inhaled and ingested uranium radionuclides). STP-use typically involves extended mouth residence (e.g. in the case of Swedish snus an average of 1 h per portion for 12-14 h/ day [43]) at habitual locations within the mouth. During this time, the user's saliva extracts constituents from the STP [16], and the radionuclide-containing saliva may be swallowed or expectorated, but can in principle act as a carrier for radionuclides from STP to mouth tissues for absorption through mucous membranes. During residence in the mouth, radionuclides in the STP may also potentially directly irradiate the tissues adjacent to the STP. Some STPs are dispersed in saliva and not designed to be expectorated; these STPs and their radionuclides will be more readily absorbed or ingested. In those STP categories that are designed for expectoration of the used product, some loose tobacco particles may be swallowed during use. When use of a non-dispersing product is complete, the remaining STP solids (which are heavily loaded with saliva) are removed by the user and discarded.

Direct radioactive exposure of oral tissues by STPs is limited

Localized irradiation of the oral tissue of STP users by α-and β-radiation from STPs during use is possible, and Hoffmann et al. [37] suggested that α-radiation emitted by STPs may contribute to an increased risk of snuff dippers for oral cancer due to concentrated irradiation of a relatively small area of the cheek and gum.

However, α-radiation can cause only localized damage owing to its short path length in air and biological matrices (< 0.1 mm) [12], and it is important to note that the dimensions of STP portions are considerably larger than this path length. Therefore only those radionuclides lying very close to the periphery of the STP portion could possibly lead to direct irradiation of oral tissue. For example, we estimate that approximately 1% of the α particles emitted within a snus pouch (i.e. those emitted near the periphery of the portion) would be capable of travelling far enough to exit the snus matrix. In addition, the average thickness of the salivary film, 0.07-0.1 mm [44] will act as an additional barrier to emitted α-radiation, further reducing the likelihood of tissue exposure from α-particles emitted within an STP.

The ability of β-radiation emitted by STP constituents to exit the STP matrix and contact the oral mucosa is highly dependent on the energy of the β-radiation emitted [45]. Low energy β-radiation emitted from 3 H and 210 Pb could penetrate only 5-6 μm from the site of emission within the STP, whereas the more energetic β-radiation from 14 C and 40 K can potentially penetrate 0.3 and ~ 5 mm respectively [45,46].

In addition to the barrier properties of the mouth's salivary film noted above, the outer layer of the oral cavity epithelium, being composed of keratin and subject to continuous sloughing, is a further physical barrier to αand β-particles. The thickness of the epithelium of the buccal mucosa (the relevant site for STP users) has been measured at around 250 μm in normal healthy subjects [47], and the most superficial keratinized squamous cells are nonvital. It is likely that STP users have somewhat thicker epithelium and a thicker keratin layer, which will physically increase the path length that emitted α-and β-particles must traverse to damage the critical cells in the basal layer. The combination of these factors make it unlikely that biologically-significant damage to oral tissue will result from STP-borne α-emitters and the majority of the β-emitters; however exposure to β-radiation emitted from 14 C and particularly 40 K in STPs may be of concern.

These estimations highlight the need for more sophisticated exposure models to assess radiological dose in STP users. These models should consider the committed effective dose arising from exposure to alpha and beta generating radionuclides; internal exposure to alpha radiation is considered more damaging than beta radiation due to the way in which energy is imparted to tissue by these two types of radiation. Several further aspects of direct irradiation need to be considered. First, the main decay mode of many radionuclides that emit α-or β-radiation can be accompanied by gamma radiation emissions. The emitted gamma radiation can introduce an additional radiation dose to the STP user, as gamma radiation can penetrate further and potentially interact with critical biological tissue; this both widens the area of potential radiation exposure but also introduces a relatively low potential for tissue damage due to the comparatively weak interaction of gamma radiation with tissue. Second, there is also potential for bremsstrahlung radiation resulting from interaction of emitted β-radiation with mercury-based dental amalgams in the mouths of some STP users. Some further, potentially important, exposure mechanisms are also important to consider in the development of a model and are described below.

Radionuclides can be extracted from STPs by users' saliva

STP users may also be exposed to radionuclides extracted from the STP by saliva during STP use. Extracted radionuclides may come into closer contact with oral tissues than those remaining within the STP [48], and therefore may more readily expose STP users to radiation. Syed et al. [48] considered 210 Po extracted in this way to be the main source of irradiation from STPs. However, for most categories of STP (other than dispersable products, for which complete ingestion can be assumed), uncertainties exist over the extent of extraction of individual radionuclides into saliva. There are few data on constituent extraction during STP use, but estimates of the extractability of 210 Po from US moist snuff in a model system using human saliva was reported as being very low, at 2-10% [48]. There are no data on the extractability of other α-emitters from STPs. It is also difficult to estimate the solubility of these species in tobacco because the exact chemical forms are unknown: recent work has demonstrated that inorganic metalloids in tobacco can be present in multiple chemical states [49] and with differing solubilities [50]. Environmental studies have shown that radium is only moderately soluble in water, but is most soluble under chloride-rich reducing aqueous systems with a high total content of dissolved solids, a condition that might relate to STPs that have a high salt and water content [51]. Environmental thorium has very low aqueous solubility [46]. Aqueous solubilities of uranium, plutonium and neptunium are low but pH dependent [52]. These data suggest limited bioavailability of these α-emitting radionuclides in tobacco, but further studies are required to draw a definitive conclusion.

Regarding the extraction of β-emitters into saliva, a study on the extractability of lead from US moist snuff and Iqmik using artificial saliva showed that lead (and hence 210 Pb) was not readily extracted (< 8%) from these STPs [53]. Similarly, no measurable level of lead extraction was found during use of snus by US snus consumers [54]. However, 14 C is incorporated chemically into the tobacco plant in several soluble organic species such as sugars, sugar esters and starches [30], and 3 H can be present as tritiated water or organic species [41]. Therefore it is likely that these two species would be bioavailable from STPs, although the extent of availability is unclear at present. There are no data on potassium extraction from tobacco; however, a study of the extraction of a range of snus constituents by users showed that ~ 30% of the sodium content was extracted [16]. Because potassium and sodium ions share very similar aqueous solubilities it is plausible to assume that potassium (and hence 40 K) extractability is also ~ 30% from snus.

Overall, these data suggest that most of the radionuclide content of STPs may remain within the STP during use, but some extraction of radionuclides into saliva, particularly 40 K, 3 H and 14 C, will occur. Once released into saliva, the radiation emitted by saliva-soluble radionuclides will have to overcome the physical shielding effects of saliva, air and non-vital epithelium cells within the oral cavity in order to encounter biologically-important tissue. However, this mechanism does represent a plausible route to the irradiation of STP users' oral cavities, particularly by 40 K and 14 C. Figure 4 illustrates that systemic dispersion of radionuclides may arise in principle from two routes during STP use: uptake through oral tissues, and swallowing tobacco and tobacco-constituents in saliva.

Systemic exposure from STP radionuclides

Radionuclides extracted from STP portions may potentially be absorbed into oral cavity tissues (Fig. 4). If tissue clearance mechanisms are relatively slow compared with STP usage duration, this may lead to a localized buildup of radionuclide in the oral tissue during use, particularly as STP users generally position the tobacco portion at a fixed location within the mouth. However, radiation exposure may be limited in this scenario, as noted above the identified STP radionuclides all have half-life times in excess of 132 days. Standard radiological models do not account for this potential source of exposure, and this is an area requiring further attention.

In contrast, the incremental exposure to radionuclides after swallowing during STP use, is within the scope of the standard radiological dose models for ingested radionuclides from the diet. Systemic dispersion of radionuclides after ingestion is well understood. Potassium (including 40 K) is almost completely absorbed after ingestion and is quickly distributed to all of the organs and tissues of the body via the bloodstream; it is eliminated from the body with a biological half-life of 30 days. However, the level of potassium in the body is under strict homeostatic control and is not influenced by environmental factors, with an adult male having a body content of 3700 Bq of 40 K [46]; hence STP use will not increase the body content of 40 K. Increased exposure to radiation from 40 K may arise in the GI tract of STP users during transit of swallowed materials; however, comparison to the recommended USA adult daily dietary intake of 4.7 g potassium [55] suggests that GI exposure of STP-sourced 40 K will be 1-2 orders of magnitude lower than dietary intake. Hence the risk of systemic exposure to 40 K from STPs will be small. In contrast, STP use can add to the body concentrations of 3 H, 14 C, and the progeny of 238 U and 232 Th, at levels corresponding to their extractability. Depending upon the effectiveness of fractional absorption from the gut there may also be some GI exposure to radionuclides that undergo extended intestinal transit. The extent of these sources of exposure is unclear, as noted above, but is likely to present a minimal increase in exposure and hence risk in comparison to dietary intake.

The risk of radiation exposure from STPs appears low

The greatest potential radiological risk from radionuclides in STPs therefore appears to be from 40 K, and to a lesser degree 14 C. Given the localized and extended time of STP use in the mouth, exposure of STP users' oral tissues to radioactivity may occur either via direct irradiation from within the STP portion or by radionuclides extracted by users' saliva. With the uncertainties surrounding STP portion size and geometry (and the resulting attenuation of radiation emitted from within STPs), and the differential extent and kinetics of extraction into saliva by users of different STPs, it is challenging to establish an accurate estimate for effective dose to the oral cavity. Clearly, more sophisticated models that account for localized exposure are desirable to quantify radionuclide exposure within the oral cavity, and their development would represent an advance in understanding the potential for oral toxicity of STP use.

Ultimately, epidemiology provides the most informative insights into the risks associated with STP use. Rosenquist et al. [56], Luo et al. [57] and Rodu and Jansson [58] have reviewed the evidence for oral cancer associated with several STP categories. These authors identified no increased risk of oral cancer for snus use by Swedes, and moist snuff and chewing tobacco use by Americans. Assuming that the radionuclide contents of STPs measured in this study are no higher than those present in STPs during the extended time periods corresponding to the epidemiological studies examined in the reviews above, then the levels of radionuclides measured in this study can be regarded as posing no significant hazard to STP users. This conclusion concurs with that expressed in the 2008 SCENIHR report [9] which stated: "the dose of ionising radiation from these sources must be considered as negligible in comparison e.g. with the natural radiation background and other sources of ionising radiations".

Regulatory implications of STP radionuclides

The FDA issued a list [5,59] of harmful or potentially harmful constituents (HPHC) in tobacco products and tobacco smoke, as required by the Federal Food, Drug, and Cosmetic Act (the FD&C Act). The list contains three radionuclides, 210 Po, 235 U and 238 U, and their presence on the list arises [60,61] from chemical data summarised in IARC Monograph 89 [1], which is in turn based on earlier reviews [62,63].

However, IARC Monograph 89 (and earlier reviews) contain factual errors relating to these uranium isotopes. Specifically, Table 3 of IARC Monograph 89 (page 58) lists 2.4 pCi/g of 235 U and 1.91 pCi/g 238 U in MS, arising from (page 85) a study by Sharma et al. [4] of the uranium content of five Indian snuff products. However, examination of the Sharma et al. study shows that the authors reported no specific data for 235 U or 238 U, instead they disclosed specific activity measurements (2.4-6.4 pCi/g) and mass concentrations (7.4-19.1 ppm) for the presence of total uranium [4]. Consequently, the presence of these uranium isotopes on the FDA list is based on flawed data summaries within the IARC monograph.

The findings of this work, which show a more complex picture of STP radiochemistry than previously considered, coupled with errors in IARC Monograph 89, may justify re-examination of the radionuclides currently identified on the FDA HPHC list.

Conclusion

The present study has revealed a more complete and complex picture of the radionuclide content of STPs than previously reported. 28 radionuclides were examined, covering all four typical sources, of which 13 were detected and quantified in STPs representing 90% market share of the US and Swedish STP markets. A number of radionuclides, such as 14 C, 3 H and 230 Th, are reported in tobacco for the first time.

The most prevalent radionuclides in these STPs were 40 K, 14 C, 210 Po and 226 Ra, (with 210 Pb although undetected in these samples, also likely to be widely present due to the secular equilibrium with 210 Po) Over half the STPs also contained 228 Th, and 8 radionuclides were identified in a small number of STPs. The activity of β-emitters was much greater than those of α-emitters, and the β-emitter 40 K was both the most radioactive species and the radionuclide present in the greatest concentration.

In contrast, the three radionuclides identified by the FDA on the HPHC list were either not detected, present in only three of 70 samples, or had activity levels fifty times lower than that of 40 K. The identities of radionuclides on the HPHC list for smokeless tobacco products may merit reconsideration in the light of these findings.

Critical review of factors potentially leading to exposure of STP users to radioactivity suggests that exposure from alpha emitters may represent minimal risk to STP users, but beta emissions from 40 K may expose STP user's oral cavities to levels of radiation during STP use. However, epidemiological evidence suggests that the levels of radionuclides measured in this study appear unlikely to present significant risks to STP users.

and 4, with individual product activity values in Additional file 1: Tables S2-S4 and the corresponding mass of these radionuclides presented in Additional file 1: Tables S5-S7. Where available, literature values of the radionuclide concentrations or activities in tobacco products are summarised inTables 2, 3 and 4.

Fig. 3
3Activities of radioisotopes (mBq/g product wwb) detected in US and Swedish STPs. Activities below LoD are displayed as 0 in the graph

Fig. 4
4Model for estimating radioactive exposure from isotopes in STPs

Table 1
1Radionuclides examined in the current studyA α-spectroscopy, G γ-spectrometryRadionuclide
Symbol
Main source
Main radioactive
emission mode

How measured
(current study)

Half-life time
Specific activity

Uranium-235

235

U
Actinium series
α
A
7.04 × 10 8 years 79.8 kBq/g

Uranium-238

238

U
Primordial
α
A
4.47 × 10 9 years 12.44 kBq/g

Uranium-234

234

U
Uranium-238 decay series
α
A
2.455 × 10 5 years 231.3 MBq/g

Thorium-234

234

Th
β −
G
24.1 days
860 TBq/g

Protactinium-234

234m

Pa
β −
G
6.7 h
74,000 TBq/g

Thorium-230

230

Th
α
A
75,440 years
762.8 MBq/g

Radium-226

226

Ra
α
A
1599 years
36 GBq/g

Lead-214

214

Pb
β −
G
26.9 min
1.213 × 10 6 TBq/g

Bismuth-214

214

Bi
α
G
28.7 min
1.63 × 10 6 TBq/g

Lead-210

210

Pb
β −
G
22.6 years
2.84 TBq/g

Polonium-210

210

Po
α
A
138.4 days
166.272 TBq/g

Thorium-232

232

Th
Primordial
α
A
1.4 × 10 10 years 4.07 kBq/g

Actinium-228

228

Ac
Thorium-232 decay series
β −
G
6.15 h
82,800 TBq/g

Thorium-228

228

Th
α
A
1.913 years
30.366 TBq/g

Lead-212

212

Pb
β −
G
10.6 h
51,407 TBq/g

Bismuth-212

212

Bi
β −
G
1.009 h
542,000 TBq/g

Tantalum-208

208

Tl
β −
G
183.2 s
1.096 × 10 7 TBq/g

Potassium-40

40

K
Primordial
β −
G
1.248 × 10 9 years 265.4 kBq/g

Carbon-14

14

C
Cosmic ray
β −
G
5700 years
170 GBq/g

Tritium

3

H
β −
G
12.32 years
356.2 TBq/g

Americium-241

241

Am
Anthropogenic
α
G
432.5 years
126.9 GBq/g

Plutonium-238

238

Pu
α
G
87.8 years
634 GBq/g

Plutonium-239

239

Pu
α
A
24,110 years
2.297 GBq/g

Plutonium-240

240

Pu
α
A
6561 years
8.404 GBq/g

Cesium-137

137

Cs
β −
G
30.08 years
3.214 TBq/g

Cesium-134

134

Cs
β −
G
2.0652 years
47.864 TBq/g

Iodine-131

131

I
β −
G
8.0252 days
4598.8 TBq/g

Cobalt-60

60

Co
β −
G
5.2749 years
41.868 TBq/g

K,60 Co, 131 I, 134 Cs, 137 Cs, 208 Tl, 210 Pb, 212 Pb, 212 Bi,214

Pb, 214 Bi, 226 Ra, 228 Ac, 234 Th, 234m Pa, 235 U and 241 Am.

Table 2
2Summary of current findings in contemporary STPs and historic values for uranium-235 and radionuclides of the uranium-238 decay seriesFor total uranium concentrations (g/g) are used, BLD-below limit of detection, BLQ-below limit of quantificationa

Table 3
3Summary of current findings in contemporary STPs and historic values for radionuclides of the thorium decay series and for potassium-40 and cosmic ray generated radionuclides BLD below limit of detection, BLQ below limit of quantificationRadionuclide Main radioactive
emission mode

Proportion

of measured STPs
in which radionu-
clide detected
Range in STPs with detectable levels

of radionuclide

Historic
radioactivity

values in tobacco
(mBq/g dwb)

Tobacco type

Country

Date

References

Radioactivity

(mBq/g WWB)

Mass (g/g WWB)

232

Th

α

1/70

1

3
× 10

−7

0.5-18.4

Various

Various
1973-2012 [20, 21, 90-92]

228

Ac

β

−

0/73

BLD

BLD

0.4-6.5
Moassel, cigarette Egypt, Iraq, Swit-

zerland
1963-2009 [28, 29, 71, 93]

228

Th

α

47/70

1-9

4-28
× 10

−17

BLQ-206

Cigarette, leaf

Russia, Brazil

1972-1994 [20, 94]

Table 4
4Summary of current findings in contemporary STPs and historic values for anthropogenic radionuclidesBLD below limit of detectionRadionu-
clide

Main radio-
active emis-
sion mode

Proportion
of meas-
ured STPs
in which
radionuclide
detected

Range in STPs with detect-
able levels of radionuclide

Historic radio-
activity values
in tobacco
mBq/g dwb

Tobacco
type

Country Date
References

Radioactiv-
ity (mBq/g
WWB)

Mass (g/g
WWB)

241

Am
α
0/73
BLD
BLD
None reported

238

Pu
α
4/73
0.4-1.1
7-17 × 10 −16 None reported

239

Pu
α
7/73
0.3-1.3
1-5 × 10 −13 0.396 (1960s)-
0.005 (1980s)

Cigarette
Finland 1985
[32]

240

Pu
α
1-4 × 10 −14

137

Cs
β −
0/73
BLD
BLD
0.1-40
Various
Various 1983-2012 [21, 35, 98, 99,
101]

134

Cs
β −
0/73
BLD
BLD
None reported

131

I
β −
0/73
BLD
BLD
None reported

60

Co
β −
0/73
BLD
BLD
None reported

Authors' contributionsKM co-directed the study, co-developed the exposure model and co-wrote the manuscript. HK project managed the study. AP co-wrote the manuscript. CL, AF, and MM planned and executed the study, JM co-developed the exposure model. BR co-directed the study and contributed to writing the manuscript. All authors read and approved the final manuscript.Competing interestsThe study was funded by British American Tobacco (BAT). At the time of the study KM, HK, CL, AF, MM and JM were employees of BAT. AP is a paid consultant to BAT. BR's research is funded in-part by unrestricted grants from Tobacco Manufacturers (including BAT) to the University of Louisville.Ethics approval and consent to participateNot applicable.Publisher's NoteSpringer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Smokeless tobacco and some tobacco-specific N-nitrosamines. IARC monographs on the evaluation of carcinogenic risks to humans. IARC Press89370LyonIARC (2007) Smokeless tobacco and some tobacco-specific N-nitros- amines. IARC monographs on the evaluation of carcinogenic risks to humans, vol 89. IARC Press, Lyon, p 370

Harm reduction in nicotine addiction: helping people who can't quit. A report by the Tobacco Advisory Group of the Royal College of Physicians. Royal College of Physicians. RCPRoyal College of Physicians (2007) Harm reduction in nicotine addic- tion: helping people who can't quit. A report by the Tobacco Advisory Group of the Royal College of Physicians. RCP, London

Toxic and carcinogenic substances in dry and moist snuff. D Hoffmann, J D Adams, D Lisk, I Fisenne, K D Brunnemann, J Natl Cancer Inst. 79Hoffmann D, Adams JD, Lisk D, Fisenne I, Brunnemann KD (1987) Toxic and carcinogenic substances in dry and moist snuff. J Natl Cancer Inst 79:1281-1286

Study of trace amounts of U in snuff. P K Sharma, N Lal, K K Nagpaul, Health Phys. 48Sharma PK, Lal N, Nagpaul KK (1985) Study of trace amounts of U in snuff. Health Phys 48:811-812

Harmful and potentially harmful constituents in tobacco products and tobacco smoke. 26Established ListHarmful and potentially harmful constituents in tobacco products and tobacco smoke; Established List (2012) FDA. http://www.fda.gov/Tobac- coProducts/GuidanceComplianceRegulatoryInformation/ucm297786. htm. Accessed 26 Sep 2016

A review of human carcinogens: personal habits and indoor combustion. IARC monographs on the evaluation of carcinogenic risks to humans. IARC Press100LyonIARC (2012) A review of human carcinogens: personal habits and indoor combustion. IARC monographs on the evaluation of carcino- genic risks to humans, vol 100E. IARC Press, Lyon, pp 265-318

Lung cancer and smoking. D K Mulvany, Lancet. 262205Mulvany DK (1953) Lung cancer and smoking. Lancet 262:205

The chemical components of tobacco and tobacco smoke. A Rodgman, T Perfetti, 978-1-4665-1548-2CRC PressBoca Raton2nd ednRodgman A, Perfetti T (2013) The chemical components of tobacco and tobacco smoke, 2nd edn. CRC Press, Boca Raton. ISBN 978-1-4665-1548-2

Scientific Committee on Emerging and Newly Identified Health Risks. Health Effects of Smokeless Tobacco Products. 27Scientific Committee on Emerging and Newly Identified Health Risks. Health Effects of Smokeless Tobacco Products (2008) SCENIHR. http://ec.europa.eu/health/ph_risk/committees/04_scenihr/docs/ scenihr_o_013.pdf. Accessed 27 Sep 2016

Radionuclides in the geosphere: sources, mobility, reactions in natural waters and interactions with solids. K H Lieser, Radiochim Acta. 7071Lieser KH (1995) Radionuclides in the geosphere: sources, mobility, reactions in natural waters and interactions with solids. Radiochim Acta 70(71):355-375

Ionizing radiation, Part 2: some internally deposited radionuclides. IARC monographs on the evaluation of carcinogenic risks to humans. IARC Press78LyonIARC (2001) Ionizing radiation, Part 2: some internally deposited radio- nuclides. IARC monographs on the evaluation of carcinogenic risks to humans, vol 78. IARC Press, Lyon

A review of human carcinogens: radiation. IARC monographs on the evaluation of carcinogenic risks to humans. IARC Press100LyonIARC (2012) A review of human carcinogens: radiation. IARC mono- graphs on the evaluation of carcinogenic risks to humans, vol 100D. IARC Press, Lyon

Polycyclic aromatic hydrocarbons in US and Swedish smokeless tobacco products. K G Mcadam, A Faizi, H Kimpton, A Porter, B Rodu, Chem Cent J. 7151McAdam KG, Faizi A, Kimpton H, Porter A, Rodu B (2013) Polycyclic aro- matic hydrocarbons in US and Swedish smokeless tobacco products. Chem Cent J 7:151

Analysis of hydrazine in smokeless tobacco products by gas chromatography-mass spectrometry. K Mcadam, H Kimpton, S Essen, P Davis, C Vas, C Wright, A Porter, B Rodu, Chem Cent J. 913McAdam K, Kimpton H, Essen S, Davis P, Vas C, Wright C, Porter A, Rodu B (2015) Analysis of hydrazine in smokeless tobacco products by gas chromatography-mass spectrometry. Chem Cent J 9:13

The acrylamide content of smokeless tobacco products. K Mcadam, H Kimpton, C Vas, D Rushforth, A Porter, B Rodu, Chem Cent J. 956McAdam K, Kimpton H, Vas C, Rushforth D, Porter A, Rodu B (2015) The acrylamide content of smokeless tobacco products. Chem Cent J 9:56

Multi-analyte approach for determining the extraction of tobacco constituents from pouched snus by consumers during use. H Digard, N Gale, G Errington, N Peters, K Mcadam, Chem Cent J. 755Digard H, Gale N, Errington G, Peters N, McAdam K (2013) Multi-analyte approach for determining the extraction of tobacco constituents from pouched snus by consumers during use. Chem Cent J 7:55

CRC handbook of chemistry and physics. Internet Version. D R Lide, Taylor and FrancisBoca Raton, FL87th ednLide DR (ed) CRC handbook of chemistry and physics. Internet Version 2007, 87th edn. Taylor and Francis, Boca Raton, FL. http://www.hbcp- netbase.com

Computational knowledge engine. Wolframalpha, 26WolframAlpha (2012) Computational knowledge engine. http://www. wolframalpha.com. Accessed 26 Sep 2016

Radioactive uranium in various Indian tobaccos and consumable products (snuff, chutta, bidi and cigarette). N Lal, P K Sharma, K K Nagpual, D Behera, S K Malik, Int J Clin Pharmacol Ther Toxicol. 25Lal N, Sharma PK, Nagpual KK, Behera D, Malik SK (1987) Radioac- tive uranium in various Indian tobaccos and consumable products (snuff, chutta, bidi and cigarette). Int J Clin Pharmacol Ther Toxicol 25:36-37

Determination of alpha emitters in Brazilian tobacco. M S Santos, A M Azeredo, D R Melo, L M Julião, J Radioanal Nucl Chem. 182Santos MS, Azeredo AM, Melo DR, Julião LM (1994) Determination of alpha emitters in Brazilian tobacco. J Radioanal Nucl Chem 182:57-62

Natural radioactivity contents in tobacco and radiation dose induced from smoking. H A Shousha, F Ahmad, Radiat Prot Dosim. 150Shousha HA, Ahmad F (2012) Natural radioactivity contents in tobacco and radiation dose induced from smoking. Radiat Prot Dosim 150:91-95

Polonium-210, a volatile radioelement in cigarettes. E P Radford, V R Hunt, Science. 143Radford EP, Hunt VR (1964) Polonium-210, a volatile radioelement in cigarettes. Science 143:247-249

Lung cancer: is the increasing incidence due to radioactive polonium in cigarettes?. J Marmorstein, Afr Med J. 79Marmorstein J (1986) Lung cancer: is the increasing incidence due to radioactive polonium in cigarettes? S Afr Med J 79:145-150

Azeredo MG (1992) 226Ra/210Pb/210Po equilibrium in tobacco leaves. J M Godoy, V A Gouveu, D R Mello, Radiat Prot Dosim. 45Godoy JM, Gouveu VA, Mello DR, Azeredo MG (1992) 226Ra/210Pb/210Po equilibrium in tobacco leaves. Radiat Prot Dosim 45:299-300

210Po and 210Pb intake by the Portuguese population: the contribution of seafood in the dietary intake of 210Po and 210Pb. F P Carvalho, Health Phys. 69Carvalho FP (1995) 210Po and 210Pb intake by the Portuguese popula- tion: the contribution of seafood in the dietary intake of 210Po and 210Pb. Health Phys 69:469-480

Inhalation of 210Po and 210Pb from cigarette smoking in Poland. B Skwarzec, J Ulatowski, D I Struminska, A Borylo, J Environ Radioact. 57Skwarzec B, Ulatowski J, Struminska DI, Borylo A (2001) Inhalation of 210Po and 210Pb from cigarette smoking in Poland. J Environ Radioact 57:221-230

Evaluation of 210Pb and 210Po in cigarette tobacco produced in Brazil. A C Peres, G Hiromoto, J Environ Radioact. 62Peres AC, Hiromoto G (2002) Evaluation of 210Pb and 210Po in ciga- rette tobacco produced in Brazil. J Environ Radioact 62:115-119

The thorium-series in cigarettes and in lungs of smokers. G Joyet, Experientia. 27Joyet G (1971) The thorium-series in cigarettes and in lungs of smokers. Experientia 27:85-89

Studying the natural radioactivity in some tobacco cigarettes imported to Iraq from unknown origins. N Farhan, Jordan Med J. 43Farhan N (2009) Studying the natural radioactivity in some tobacco cigarettes imported to Iraq from unknown origins. Jordan Med J 43:83-90

The distribution pattern of carbon-14 assimilated by a single leaf in tobacco plant. T Yamamoto, Plant Cell Physiol. 8Yamamoto T (1967) The distribution pattern of carbon-14 assimilated by a single leaf in tobacco plant. Plant Cell Physiol 8:353-362

Isotopic evidence of plutonium release into the environment from the Fukushima DNPP accident. J Zheng, K Tagami, Y Watanabe, S Uchida, T Aono, N Ishii, S Yoshida, Y Kubota, S Fuma, S Ihara, 10.1038/srep00304Sci Rep. 2204Zheng J, Tagami K, Watanabe Y, Uchida S, Aono T, Ishii N, Yoshida S, Kubota Y, Fuma S, Ihara S (2012) Isotopic evidence of plutonium release into the environment from the Fukushima DNPP accident. Sci Rep 2:204. https://doi.org/10.1038/srep00304

Plutonium-239, 240Pu and 210Pu contents of tobacco and cigarette smoke. H Mussalo-Rauhamaa, T Jaakkola, Health Phys. 49Mussalo-Rauhamaa H, Jaakkola T (1985) Plutonium-239, 240Pu and 210Pu contents of tobacco and cigarette smoke. Health Phys 49:296-301

Determination of 16 elements in tobacco by neutron activation analysis. A Wyttenbach, S Bajo, A Haekkinen, Beitr Tabakforsch Int. 8Wyttenbach A, Bajo S, Haekkinen A (1976) Determination of 16 ele- ments in tobacco by neutron activation analysis. Beitr Tabakforsch Int 8:247-249

Naturally occurring alpha activity of cigarette tobaccos. R C Turner, J M Radley, Lancet. 275Turner RC, Radley JM (1960) Naturally occurring alpha activity of ciga- rette tobaccos. Lancet 275:1197-1198

Radiation dose from cigarette tobacco. C Papasterfanou, Radiat Prot Dosim. 123Papasterfanou C (2007) Radiation dose from cigarette tobacco. Radiat Prot Dosim 123:68-73

Radioactivity of tobacco leaves and radiation dose induced from smoking. C Papasterfanou, Int J Environ Res Public Health. 6Papasterfanou C (2009) Radioactivity of tobacco leaves and radiation dose induced from smoking. Int J Environ Res Public Health 6:558-567

Carcinogenic agents in snuff. D Hoffmann, N Harley, I Fisenne, J Adams, K Brunneman, JNCI. 76Hoffmann D, Harley N, Fisenne I, Adams J, Brunneman K (1986) Carcino- genic agents in snuff. JNCI 76:435-437

Comparative chemical analysis of different lots of Ariva ™. 26Comparative chemical analysis of different lots of Ariva ™ (2002) FDA. http://www.fda.gov/ohrms/DOCKETS/dailys/02/Nov02/111502/02p- 0075-sup0002-02-attach-a-vol2.pdf. Accessed 26 Sep 2016

Basis for the ICRP's age-specific biokinetic model for uranium. R W Leggett, Health Phys. 67Leggett RW (1994) Basis for the ICRP's age-specific biokinetic model for uranium. Health Phys 67:589-610

Cancer risk coefficients for environmental exposure to radionuclides. EPA 402-R-99-001Oak Ridge National LaboratoryUSEPAUSEPA. Cancer risk coefficients for environmental exposure to radio- nuclides, federal guidance report no. 13, 1999. EPA 402-R-99-001. Oak Ridge National Laboratory

Report of the United Nations scientific committee on the effects of atomic radiation to the general assembly. 26UNSCEARUNSCEAR. Report of the United Nations scientific committee on the effects of atomic radiation to the general assembly. http://www. unscear.org/docs/publications/2000/UNSCEAR_2000_Report_Vol.I.pdf. Accessed 26 Sep 2016

Human alimentary tract model for radiological protection. ICRP Publication. 100ElsevierICRPICRP (2006) Human alimentary tract model for radiological protection, ICRP Publication 100. Elsevier, Amsterdam

Patterns and behavior of snus consumption in Sweden. H Digard, G Errington, A Richter, K Mcadam, Nicotine Tob Res. 11Digard H, Errington G, Richter A, McAdam K (2009) Patterns and behav- ior of snus consumption in Sweden. Nicotine Tob Res 11:1175-1181

The surface area of the adult human mouth and thickness of the salivary film covering the teeth and oral mucosa. Lmc Collins, C Dawes, J Dent Res. 66Collins LMC, Dawes C (1987) The surface area of the adult human mouth and thickness of the salivary film covering the teeth and oral mucosa. J Dent Res 66:1300-1302

Woods Hole Oceanographic Institute (2012) Isotope safety data sheets. Woods Hole Oceanographic Institute (2012) Isotope safety data sheets. http://ehs.whoi.edu/ehs/radsafety/isotope.pdf. Accessed 26 Sep 2016

Human health fact sheet. Accessed. 2640Argonne National LaboratoryHuman health fact sheet, potassium-40 (2005) Argonne National Laboratory. https://www.remm.nlm.gov/ANL_ContaminantFactSheets_ All_070418.pdf. Accessed 26 Sep 2016

Measurement of epithelial thickness within the oral cavity using optical coherence tomography. S Prestin, S I Rothschild, C S Betz, M Kraft, 10.1002/hedPrestin S, Rothschild SI, Betz CS, Kraft M (2012) Measurement of epithe- lial thickness within the oral cavity using optical coherence tomogra- phy. Head Neck. https://doi.org/10.1002/hed

Leaching of 210 Po in human saliva from smokeless tobacco. U-F Syed, Bari A Husain, L , J Radioanal Nucl Chem. 281Syed U-F, Bari A, Husain L (2009) Leaching of 210 Po in human saliva from smokeless tobacco. J Radioanal Nucl Chem 281:541-546

Arsenic speciation in tobacco and cigarette smoke. C Liu, C G Wright, K G Mcadam, C Taebunpakul, J Heroult, J Braybrook, H Goenaga-Infante, Beitr Tabakforsch Int. 25Liu C, Wright CG, McAdam KG, Taebunpakul C, Heroult J, Braybrook J, Goenaga-Infante H (2012) Arsenic speciation in tobacco and cigarette smoke. Beitr Tabakforsch Int 25:375-380

Complementary HPLC-ICP-MS and synchrotron X-ray absorption spectroscopy for speciation analysis of chromium in tobacco samples. S Cuello, J Entwisle, J Benning, C Liu, S Coburn, K G Mcadam, J Braybrook, H Goenaga-Infante, 10.1039/c5ja00442jJ Anal At Spectrom. Cuello S, Entwisle J, Benning J, Liu C, Coburn S, McAdam KG, Braybrook J, Goenaga-Infante H (2015) Complementary HPLC-ICP-MS and syn- chrotron X-ray absorption spectroscopy for speciation analysis of chro- mium in tobacco samples. J Anal At Spectrom. https://doi.org/10.1039/ c5ja00442j

Natural radioactivity in ground water: a review. U.S. Geological Survey national water summary 1986. Groundwater quality: hydrologic conditions and events, US Geological Survey water supply paper 2325. O S Zapecza, Z Szabo, Zapecza OS, Szabo Z (1987) Natural radioactivity in ground water: a review. U.S. Geological Survey national water summary 1986. Ground- water quality: hydrologic conditions and events, US Geological Survey water supply paper 2325. pp 50-57

Geochemical modeling of solubility and speciation of uranium, neptunium, and plutonium. (YM). Z Yu, Y Lin, K Johannesson, A Smiecinski, K J Stetzenbach, Paper 66Yu Z, Lin Y, Johannesson K, Smiecinski A, Stetzenbach KJ (2007) Geo- chemical modeling of solubility and speciation of uranium, neptunium, and plutonium. (YM). Paper 66. http://digitalscholarship.unlv.edu/ yucca_mtn_pubs/66. Accessed 26 Sept 2016

Analysis of toxic metals in commercial moist snuff and Alaskan iqmik. R S Pappas, S B Stanfill, C H Watson, D L Ashley, J Anal Toxicol. 32Pappas RS, Stanfill SB, Watson CH, Ashley DL (2008) Analysis of toxic metals in commercial moist snuff and Alaskan iqmik. J Anal Toxicol 32:281-291

Assessment of mouth-level exposure to tobacco constituents in U.S. snus consumers. J W Caraway, P X Chen, 10.1093/ntr/nts187Nicotine Tob Res. Caraway JW, Chen PX (2012) Assessment of mouth-level exposure to tobacco constituents in U.S. snus consumers. Nicotine Tob Res. https:// doi.org/10.1093/ntr/nts187

National Institute of Health. US National Library of Medicine: potassium in diet. 55. National Institute of Health. US National Library of Medicine: potassium in diet. https://medlineplus.gov/ency/article/002413.htm. Accessed 26 Sep 2016

Use of moist snuff, smoking and alcohol consumption in the aetiology of oral and oropharyngeal cell carcinoma. A population-based case-control study in southern Sweden. K Rosenquist, J Wennerberg, E B Schildt, Acta Otolaryngol. 125Rosenquist K, Wennerberg J, Schildt EB et al (2005) Use of moist snuff, smoking and alcohol consumption in the aetiology of oral and oro- pharyngeal cell carcinoma. A population-based case-control study in southern Sweden. Acta Otolaryngol 125:991-998

Oral use of Swedish moist snuff and risk for cancer of the mouth, lung and pancreas among male construction workers. J Luo, W Ye, K Zendehdel, Lancet. 369Luo J, Ye W, Zendehdel K et al (2007) Oral use of Swedish moist snuff and risk for cancer of the mouth, lung and pancreas among male construction workers. Lancet 369:2015-2020

Smokeless tobacco and oral cancer: a review of the risks and determinants. B Rodu, C Jansson, Crit Rev Oral Biol Med. 15Rodu B, Jansson C (2004) Smokeless tobacco and oral cancer: a review of the risks and determinants. Crit Rev Oral Biol Med 15:252-263

Draft guidance for industry: reporting harmful and potentially harmful constituents in tobacco products and tobacco smoke under the federal food, drug, and cosmetic act (2012) FDA. Federal Register. 77Draft guidance for industry: reporting harmful and potentially harmful constituents in tobacco products and tobacco smoke under the federal food, drug, and cosmetic act (2012) FDA. Federal Register/vol. 77, no. 64. https://www.federalregister.gov/docu- ments/2012/04/03/2012-7766/draft-guidance-for-industry-reporting- harmful-and-potentially-harmful-constituents-in-tobacco. Accessed 27 Sep 2016

FDA briefing information for the June 8-9 meeting of the tobacco constituents subcommittee of the TPSAC (memorandum). Director, Center for Tobacco Products. FDA briefing information for the June 8-9 meeting of the tobacco con- stituents subcommittee of the TPSAC (memorandum). Director, Center for Tobacco Products (2010) FDA. http://www.fda.gov/downloads/Advi- soryCommittees/CommitteesMeetingMaterials/TobaccoProductsScien- tificAdvisoryCommittee/UCM212300.pdf. Accessed 26 Sep 2016

The subcommittee's edited version of the draft initial list of harmful/potentially harmful constituents in tobacco smoke or smokeless tobacco products (PDF) (2010) FDA. 26The subcommittee's edited version of the draft initial list of harm- ful/potentially harmful constituents in tobacco smoke or smokeless tobacco products (PDF) (2010) FDA. http://www.fda.gov/downloads/ AdvisoryCommittees/CommitteesMeetingMaterials/TobaccoProd- uctsScientificAdvisoryCommittee/UCM219548.pdf. Accessed 26 Sep 2016

Chemical composition of smokeless tobacco products. In: Smokeless tobacco or health. An international perspective. K D Brunnemann, D Hoffmann, Smoking and tobacco control monograph. 2NIH PublNational Cancer InstituteBrunnemann KD, Hoffmann D (1993) Chemical composition of smoke- less tobacco products. In: Smokeless tobacco or health. An interna- tional perspective. Smoking and tobacco control monograph, vol. 2. NIH Publ. No. 93-3461, National Cancer Institute, Bethesda, pp 96-108

On the control of toxic substances in smokeless tobacco. D Hoffmann, M V Djordjevic, K D Brunnemann, J Smok Relat Dis. 2Hoffmann D, Djordjevic MV, Brunnemann KD (1991) On the control of toxic substances in smokeless tobacco. J Smok Relat Dis 2:165-172

Analysis of tobacco by instrumental epithermal neutron activation analysis. P Faanhof, H A Das, Radiochem Radioanal Lett. 41Faanhof P, Das HA (1979) Analysis of tobacco by instrumental epither- mal neutron activation analysis. Radiochem Radioanal Lett 41:367-374

Determination of the uranium content in some Indian cigarettes. S K Chakarvarti, J Dhiman, K K Nagpaul, Health Phys. 36Chakarvarti SK, Dhiman J, Nagpaul KK (1979) Determination of the uranium content in some Indian cigarettes. Health Phys 36:638-640

Analysis of trace uranium in Indian tobacco samples. A Rawat, P J Jojo, A Kumar, R Prasad, J Radioanal Nucl Chem. 166Rawat A, Jojo PJ, Kumar A, Prasad R (1992) Analysis of trace uranium in Indian tobacco samples. J Radioanal Nucl Chem 166:365-372

Insights from analysis for harmful and potentially harmful constituents (HPHCs) in tobacco products. M J Oldham, D J Desoi, L T Rimmer, K A Wagner, M J Morton, Regul Toxicol Pharmacol. 70Oldham MJ, DeSoi DJ, Rimmer LT, Wagner KA, Morton MJ (2014) Insights from analysis for harmful and potentially harmful constituents (HPHCs) in tobacco products. Regul Toxicol Pharmacol 70:138-148

Characterization of SPECTRUM variable nicotine research cigarettes. P Richter, R S Pappas, R Bravo, J G Lisko, M Damian, N Gonzalez-Jimenez, N Gray, L M Keong, J B Kimbrell, P Kuklenyik, T S Lawler, G E Lee, M Mendez, J Perez, S Smith, H Tran, R Tyx, C H Watson, 10.18001/TRS.2.2.1Tob Regul Sci. 22Richter P, Pappas RS, Bravo R, Lisko JG, Damian M, Gonzalez-Jimenez N, Gray N, Keong LM, Kimbrell JB, Kuklenyik P, Lawler TS, Lee GE, Mendez M, Perez J, Smith S, Tran H, Tyx R, Watson CH (2016) Characterization of SPECTRUM variable nicotine research cigarettes. Tob Regul Sci 2(2):94-105. https://doi.org/10.18001/TRS.2.2.1

Determination of toxic metals in little cigar tobacco with 'Triple Quad' ICP-MS. R S Pappas, N Martone, N Gonzalez-Jimenez, M R Fresquez, C H Watson, J Anal Toxicol. 39Pappas RS, Martone N, Gonzalez-Jimenez N, Fresquez MR, Watson CH (2015) Determination of toxic metals in little cigar tobacco with 'Triple Quad' ICP-MS. J Anal Toxicol 39:347-352

Determination of environmental actinide nuclides and lead-210 (polonium-210) by low-energy photon spectrometry with alpha-spectrometry. M Sakanoue, M Yamamoto, K Komura, J Radioanal Nucl Chem. 115Sakanoue M, Yamamoto M, Komura K (1987) Determination of environ- mental actinide nuclides and lead-210 (polonium-210) by low-energy photon spectrometry with alpha-spectrometry. J Radioanal Nucl Chem 115:71-82

Natural radioactivity contents in tobacco. N A El-Aziz, Aem Khater, Al-Sewaidan Ha, Int Congr Ser. 1276El-Aziz NA, Khater AEM, Al-Sewaidan HA (2005) Natural radioactivity contents in tobacco. Int Congr Ser 1276:407-408

Radiation chemistry: radium alpha-activity of cigarette and pipe tobacco ash. E T Pallister, J H Green, Nature. 195Pallister ET, Green JH (1962) Radiation chemistry: radium alpha-activity of cigarette and pipe tobacco ash. Nature 195:487-488

Polonium-210 in tobacco products and human tissues. E S Ferri, E J Baratta, Radiol Health Data Rep. 7Ferri ES, Baratta EJ (1966) Polonium-210 in tobacco products and human tissues. Radiol Health Data Rep 7:485-488

Polonium 210 in tobacco, cigarette smoke, and selected humans. E S Ferri, E J Baratta, Public Health Rep. 81Ferri ES, Baratta EJ (1966) Polonium 210 in tobacco, cigarette smoke, and selected humans. Public Health Rep 81:121-127

Radium-226 and polonium-210 in Burley and cigar wrapper tobacco. T C Tso, N H Harley, L T Alexander, 1163TobaccoTso TC, Harley NH, Alexander LT (1966) Radium-226 and polonium-210 in Burley and cigar wrapper tobacco. Tobacco 1163:28-29

Lead-210 and polonium-210 contents in certain types of Czechoslovak cigarettes. H Solnicka, R Bischof, Cas Lek Cesk. 109654translationSolnicka H, Bischof R (1970) Lead-210 and polonium-210 contents in certain types of Czechoslovak cigarettes. Cas Lek Cesk 109:654 (translation)

Content and distribution of radium-226 and polonium-210 in tobacco. M Cui, Z Yao, X Yan, A Shi, Fushe Fanghu. 3Cui M, Yao Z, Yan X, Shi A (1983) Content and distribution of radium-226 and polonium-210 in tobacco. Fushe Fanghu 3:218-222

International Atomic Energy Agency, Vienna 79. Purkayastha BC, Bhattacharyya DK (1975) Estimation of rare and radioactive constituents in samples of Indian tobacco with the aid of low-level beta-counter. S L Simon, S A Ibrahim, Series No. 310The environmental behaviour of radium. 1Technical ReportBiological uptake by terrestrial plantsSimon SL, Ibrahim SA (1990) Biological uptake by terrestrial plants. In: The environmental behaviour of radium, vol 1, Chap 5-6. Technical Report Series No. 310. International Atomic Energy Agency, Vienna 79. Purkayastha BC, Bhattacharyya DK (1975) Estimation of rare and radioactive constituents in samples of Indian tobacco with the aid of low-level beta-counter. J Radioanal Chem 27:345-351

Pb-210 concentrations in cigarettes tobaccos and radiation doses to the smokers. S N Tahir, A S Alaamer, Radiat Prot Dosim. 130Tahir SN, Alaamer AS (2008) Pb-210 concentrations in cigarettes tobac- cos and radiation doses to the smokers. Radiat Prot Dosim 130:389-391

. A Savidou, K Kehagia, K Eleftheriadis, 210Savidou A, Kehagia K, Eleftheriadis K (2006) Concentration levels of 210

Pb and 210 Po in dry tobacco leaves in Greece. J Environ Radioact. 85Pb and 210 Po in dry tobacco leaves in Greece. J Environ Radioact 85:94-102

Po and 210 Pb concentration of cigarettes traded in Hungary and their estimated dose contribution due to smoking. T Kovacs, J Somali, K Nagy, G Szeiler, Radiat Meas. 42Kovacs T, Somali J, Nagy K, Szeiler G (2007) 210 Po and 210 Pb concentra- tion of cigarettes traded in Hungary and their estimated dose contribu- tion due to smoking. Radiat Meas 42:1737-1741

Polonium-210 and lead-210 in food and tobacco products: a review of parameters and an estimate of potential exposure and dose. ORNL/TM-8831. A P Watson, Oak Ridge National Laboratory, Oak RidgeWatson AP (1983) Polonium-210 and lead-210 in food and tobacco products: a review of parameters and an estimate of potential exposure and dose. ORNL/TM-8831. Oak Ridge National Laboratory, Oak Ridge

Lead-210 and polonium-210 in tissues of cigarette smokers. R B Holtzman, F H Ilcewicz, Science. 153Holtzman RB, Ilcewicz FH (1966) Lead-210 and polonium-210 in tissues of cigarette smokers. Science 153:1259-1260

Polonium-210 in the environment and in the human organism. Y D Parfenov, At Energy Rev. 12Parfenov YD (1974) Polonium-210 in the environment and in the human organism. At Energy Rev 12:75-143

Lead-210 in tobacco and cigarette smoke. E S Ferri, H Christiansen, Public Health Rep. 82Ferri ES, Christiansen H (1967) Lead-210 in tobacco and cigarette smoke. Public Health Rep 82:828-832

Determination of polonium-210 in cigarettes from Syria. K Batarekh, D K Teherani, J Radioanal Nucl Chem. 117Batarekh K, Teherani DK (2005) Determination of polonium-210 in cigarettes from Syria. J Radioanal Nucl Chem 117:75-80

. C H Colangelo, M R Huguet, M A Palacios, A A Oliveira, 210Colangelo CH, Huguet MR, Palacios MA, Oliveira AA (1992) Levels of 210

Po in some beverages and in tobacco. J Radioanal Nucl Chem Lett. 166Po in some beverages and in tobacco. J Radioanal Nucl Chem Lett 166:195-202

Medical consequences of polonium in snus. C Samuelsson, Läkartidningen. 86translationSamuelsson C (1989) Medical consequences of polonium in snus. Läkartidningen 86:2290-2291 (translation)

Determination of trace elements in Iranian cigarette tobacco by neutron activation analysis. Z Abedinzadeh, B Parsa, J Radioanal Chem. 14Abedinzadeh Z, Parsa B (1973) Determination of trace elements in Iranian cigarette tobacco by neutron activation analysis. J Radioanal Chem 14:139-145

Neutron activation analysis of an Iranian cigarette and its smoke. Z Abedinzadeh, M Razeghi, B Parsa, J Radioanal Chem. 35Abedinzadeh Z, Razeghi M, Parsa B (1977) Neutron activation analysis of an Iranian cigarette and its smoke. J Radioanal Chem 35:373-376

Trace element characteristics of Indian cigarette tobacco by instrumental neutron activation analysis. A N Garg, V Singh, N L Chutke, M N Ambulkar, J Radioanal Nucl Chem. 195Garg AN, Singh V, Chutke NL, Ambulkar MN (1995) Trace element characteristics of Indian cigarette tobacco by instrumental neutron activation analysis. J Radioanal Nucl Chem 195:161-172

Alpha-particle activity and free radicals from tobacco. E Marsden, M A Collins, Nature. 198Marsden E, Collins MA (1963) Alpha-particle activity and free radicals from tobacco. Nature 198:962-964

Thorium-228 content in tobacco and the human body. A A Petushkov, Mr, A I Medvedovskii, Gig Sanit. 37Petushkov AA, Zel'tser MR, Medvedovskii AI (1972) Thorium-228 con- tent in tobacco and the human body. Gig Sanit 37:113-114

Natural radioactivity in tobacco and tobacco smoke. V C Runeckles, Nature. 191Runeckles VC (1961) Natural radioactivity in tobacco and tobacco smoke. Nature 191:322-325

Radioactivity of tobacco. B Bonev, P Karmuzova, Nauchni Tr Vissh Inst Khranit Vkusova Prom-st Plovdiv. 122translationBonev B, Karmuzova P (1975) Radioactivity of tobacco. Nauchni Tr Vissh Inst Khranit Vkusova Prom-st Plovdiv 122:225-231 (translation)

Measurement of environmental radioactivities. S Sakamoto, K Matsumura, Tokai Daigaku Kiyo Kogakubu. 1981115005940Sakamoto S, Matsumura K (1981) Measurement of environmental radioactivities. Tokai Daigaku Kiyo Kogakubu, vol 1981, no 1, pp 53-56. https://inis.iaea.org/search.aspx?orig_q=RN:15005940

Caesium-137 and strontium-90 from nuclear weapon fall-out using tobacco as an example. E Fischer, J A Schmidt, W H Kalus, R Schelenz, Z Lebensm Untersuch Forsch. 176translation from GermanFischer E, Schmidt JA, Kalus WH, Schelenz R (1983) Caesium-137 and strontium-90 from nuclear weapon fall-out using tobacco as an example. Z Lebensm Untersuch Forsch 176:27-31 (translation from German)

Doses from radiocaesium and 40 K activities found in some tobacco leaves and cigarettes. J J Fletcher, Appl Radiat Isot. 45Fletcher JJ (1994) Doses from radiocaesium and 40 K activities found in some tobacco leaves and cigarettes. Appl Radiat Isot 45:133-134

40 K activities and potassium concentrations in tobacco samples of Mexican cigarettes. T Martinez, M Navarrete, L Cabrera, F Juarez, A Ramos, K Vazquez, J Radioanal Nucl Chem. 273Martinez T, Navarrete M, Cabrera L, Juarez F, Ramos A, Vazquez K (2007) 40 K activities and potassium concentrations in tobacco samples of Mexican cigarettes. J Radioanal Nucl Chem 273:569-572

Fate of potassium and caesium-137 during the machine smoking of cigarettes. R Schelenz, E Fischer, Z Lebensm Untersuch Forsch. 178Schelenz R, Fischer E (1984) Fate of potassium and caesium-137 during the machine smoking of cigarettes. Z Lebensm Untersuch Forsch 178:118-119