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Changing the Color Theme in Office 2013 including Outlook 2013, Word, Excel was not possible until the final RTM release which is not available on Technet and Volume Licenses sites at Microsoft. This has been a very sore subject with the Office 2013 Preview as the default White theme has been quite painful on the eyes. So changing the colors was the first thing I checked on after installing the RTM and I'm excited to announce it IS possible and here's how to do it.
Again – This only works with Office 2013 RTM "Release to Manufacturer". You cannot change the theme colors in the Preview.
Launch Outlook 2013 (or Word or Excel…) and Click File, Options
On the General Tab, click the down arrow under Office Theme to choose a new Office Color. White, Light Gray, and Dark Gray are the available options.
Once you choose a new Color Theme, it will take effect in all Office apps including Outlook 2013, Word 2013, Excel 2013, etc. Here're a few screenshots of the new look.
Click to enlarge:
Personally, my favorite is the Light Gray however I was also getting quite used to the White Color Theme also. Microsoft is apparently trying to maintain a particular look here being that the colors are relatively limited vs. Office 2010.


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We Are All Media Now
Looks as if Al Gore is still firmly stuck on the 20th-century end of his "bridge to the 21st century." In attempting (and failing) to bar "media" from his speech at the CTIA trade show next week, he showed his total failure to understand the basic 21st-century truth being repeated ad nauseam at South by Southwest this week: in the era of Twitter, we are all media.
Here's Gore's tantrum in a nutshell: The former Vice President is giving a speech in front of 4,000 people at the CTIA Wireless cell-phone trade show, and he tried to ban people with media badges from the room. After several Web sites pointed out that a room of 4,000 people at a cell-phone convention is a room with at least 3,000 people live-blogging on Twitter, he relented.
After the spat appeared on the popular news-industry blog Romenesko, a few other journalists guessed who Gore's real target might be. According to them, he has a vendetta against certain large, old-school media organizations that he blames for causing him to lose the 2000 election; his media ban was aimed at them. Gore is probably a little cannier than I first thought, because what he may be exploiting is the difference between three overlapping classes of media right now: just plain media, professional media, and something I'll call certifiable media. (Pun intended.) With Twitter, everyone is media. Automatically. Anyone who recounts any experience in 140 characters gets the report published on the Web, where it's as searchable as anything I write. Instant media.
Twitter, with its millions of users, is a democratic step way beyond CNN's "iReporters," Al Gore's own Current TV, and venerable community-run Indymedia sites, all of whom harness the work of independent, volunteer writers and build what looks like a professional media site from them.
I like Twitter a heck of a lot more than CNN, and a lot more than Gore's approach, too, and here's why: It doesn't make any money. CNN is basically using its iReporters as unpaid labor to generate a profit. In my mind, if you're helping someone generate a profit, you should be getting paid for it.
So here we get to professional media: people who are being paid for it. That's a key part of CTIA's definition, and it's useful to have professional media. Getting paid tends to give us the time to develop expertise beyond the amateur level. Since I've been studying cell phones for almost five years, I've talked on several hundred different cell phones. I probably couldn't have done that if I had a different day job.
But professional media is different from certifiable mediawhich is actually the most important group. Certifiable media is certifiable because it represents the people in many places and events. And here we get to Al Gore.
There's only a certain number of seats in the White House press room, in Apple's briefing room, or even in the hall where Al Gore is giving his keynote. Bill Gates has only a certain number of hours in a day to give interviews. In a 15-minute session, a CEO can answer only a certain number of questions. There will be scarcity of access as long as there is scarcity of space and time.
As a society, we want those seats to be taken up by people who represent as many other people as possible, and who do it with as much intelligence as possible. The controlling question then becomes not whether you're paid, but how many readers you have: Whom do you represent?
Web analytics makes this meritocratic. Let's show everyone our page view counts. Let's use those to judge whether we represent a grand enough constituency to be allowed in the door. We need to make sure that primary media sources, those who represent the largest audiences, get access to original information. From there, the word will spread.
The Internet has democratized punditry. New technologies and the popularization of micro-media have made the media landscape far more resilient than in the past. Unless you're willing to make everyone you talk to sign a loyalty oath, information will get out. That's just the way life goes on the other end of the bridge to the 21st century.
Follow me on Twitter! http://twitter.com/saschasegan
More Sascha Segan:
Don't Let the Sprint/T-Mobile Zombie Merger Rise Again
iPhone X Has One Small Problem
Will Apple Address Its Global Problem at the iPhone Event?
Equifax Must Pay
Huawei's Irrelevant Success

subset: commoncrawl


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Science in Christian Perspective
Letter to the Editor
Response to Mr. Cole
From: JASA 20 (June 1968): 61
I am pleased to respond to Mr. Cole's letter. I'm sure that in it he has also voiced the thoughts and opinions of others. Indeed, some clarification of points I made in the article are in order. My numbering will follow those of his letter.
1. The phrase "male and female" seemed to precede the phrase "Adam and Eve" in my Bible! It points up that this anthropologist's knowledge of the Hebrew language is as ignorant as most theologians' knowledge of anthropology. Since the morphology of early Hebrew poetry (mythology) reflects the Hebrew's worldview and requires an equilibrium, male and female are neatly balanced by Adam and Eve, both being the same. This is true throughout Genesis 1.
2. I quite agree with some of his points. I would stress my point again: that there were humans in the world other than the children of Adam and Eve whom the latter's children married. To say, "if there was a pre-Adamic race, it appears that it no longer existed since Eve received her name 'Because she was the mother of all living"', is a good example of closing one's eyes to the overwhelming evidence to the contrary in God's revelation in nature.
3. Since evolution is an abstract concept, it can be used in almost any way one wants to use it.
4. The dates given in chart 3 are approximations with the exception of Zinjantbropus, which as a stable date, is 1,750,000 -± 300 years. In capsulating some of the material relating to Zinjanthropus and Homo habilis, I purposely omitted Australopithecus since I considered "it" as "ape" rather than human. I should like to develop this point now: a) It must be kept in mind that there are 77 Australopithecines in Africa alone. These are divided into two groups: "Australopithecus africanus and Austrolopithecus robustus. The " true" Australopithecus, the one giving the name to the group, belongs to the former; Zinjanthropus belongs to the latter. In fact, he is Mr. Robustus himself. Both groups were contemporary. The majority of schol ars feel that Australopithecus africarms was an ape, while Zinjanthropus was hominid or human. The former, A. africanus was probably a random tool-maker though some question even this capability; the latter, A. robustus, was a symbol-maker who purposefully made tools. Although Leaky considers Homo habilis an Australopithecine, Clark, for example, doesn't and it will probably take a while before one will definitely know about the "handy man". He is up for grabs.
Schenk's book, published in 1961 is hopelessly out of date.
Wadjak I and 11 found by DuBois in 1892 were considered as on the same geological level as Pithecanthropus erectus, now called Homo erectus. A re-study of the stratigraphy of Java in the 1930's showed up this error with the result that the Wadjak's are Upper-Pleistocene and Pithecantbropus is Middle Pleistocene, rather than lower as DuBois originally placed him. The Wadjaks to my knowledge, were never placed lower than P. erectus.
5. Mr. Horner was careful NOT to say "that tools is an evidence of being a Homo sapiens". That is contrary to the facts. The point made was that man is unique in his symbol-making ability. The difference between a symbol-maker and a tool-maker is the difference between conceptual and perceptual ability. Man enjoys both. The chimps, porpoises and dogs among other animals enjoy the latter. The choppers made by Zinjantbropus, the religious ceremonies of Peking and Cro-Magnon man are a difference in concept compared to the sharpening of sticks to make tools and playing tic-tac-toe by the chimp, which are perceptual. Man can not live by tools alone!
6. Answered in No. 5. This relationship is not
Suggested Bibliography
Adler, Mortimer, The Difference ot Man and the Difference it
Holt, Rinehart and Winston, New York, 1967.
Buettner-Janusch, J., Origins of Man, Wiley, 1965.
Le Gros Clark, W., Man-Apes or Ape-Men? Holt, Rinehart and Winston, 1967.
George R. Horner
Department of Anthropology, Eastern Nazarene College, Wollaston, Mass.


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PORT CHARLOTTE, Fla. (AP) - The importance of good baserunning is a major point of emphasis for the Tampa Bay Rays in spring training and Manny Ramirez is paying attention.
The slugger had two hits, drove in a run and played left field for the first time with his new team during a 6-5 loss to a Pittsburgh Pirates split squad on Monday.
Ramirez, signed this month to a $2 million, one-year contract, also made a good impression by scoring from third base on a second-inning wild pitch, sliding into the plate to the delight of the crowd at Charlotte Sports Park.
``We've talked about baserunning almost 100 percent of the time during practice in the mornings,' manager Joe Maddon said. ``I liked what he did right there. He's alert. He's buying into everything we're trying to do here, and I liked it a lot.'
Garrett Atkins hit a three-run homer for Pittsburgh, delivering the big blow in a five-run fourth inning against right-hander Mike Ekstrom.
Josh Fields had a RBI single for the Pirates, and Chris Snyder scored the winning run on wild pitch during an error-filled seventh inning.
Ramirez started in left field for the Rays after making his spring debut as a designated hitter on Monday. He went 2 for 2, including a RBI single off Fernando Nieve, and scored Tampa Bay's first run when he scooted home on Bryan Morris' wild pitch.
``The game says, `play ball,'' Ramirez said, shrugging off his heads-up baserunning. ``I just go and play.'
It was a relatively quiet day in left for the 12-time All-Star, who only had two balls hit his way. He caught Atkins' line drive to end the second inning, then fielded Neil Walker's fourth-inning single before leaving the the game.
The Rays intend to use Ramirez as the regular DH. Still, they also want him to play some in the outfield during spring training so he's prepared for the possibility they'll need him to spell Johnny Damon on occasion during the season.
``He did fine. A couple of chances out there,' Maddon said. ``I thought he looked really good at the plate. Line drive into left-center, line drive into right-center. I liked his approach at the plate. He looked really good.'
Right-hander Jeff Niemann made his first spring start for the Rays, pitching one scoreless inning. Relievers Kyle Farnsworth and Joel Peralta, signed this winner to help rebuild a bullpen depleted by free agency, both worked scoreless innings, too.
Peralta hit in the back of the left calf by a line drive off the bat of Alex Presley, but was able to throw the runner out at first and finish the inning.
``It hit him pretty squarely in the calf, and we were just concerned that it was going to get too tight,' Maddon said. ``But apparently he was fine. He pitched through it. I've not heard anything negative ... so I imagine it's all good.'
Niemann has been one of Tampa Bay's most consistent pitchers over the past two seasons. He was 6-0 over his first 12 starts of 2010 and was 10-3 with a 3.12 ERA before being placed on the 15-day disabled list with a right shoulder strain on Aug. 9.
He struggled after returning in late August, going 2-5 with a 9.82 ERA in eight appearances, including seven starts.
Nevertheless, the 6-foot-9 right-hander is one of eight big league pitchers with at least 12 victories and a .600 winning percentage each of the past two seasons. The others are Roy Halladay, Tim Lincecum, CC Sabatahia, Jon Lester, Justin Verlander, Adam Wainwright and Chris Carpenter.
``It's always nice to get off to a good start,' Niemann said, adding that the 12-pitch, one-two-three inning was a nice gift on his 28th birthday.
NOTES: Damon, Ben Zobrist and Justin Ruggiano also drove in runs for the Rays. ... Morris, 9-4 with a 3.57 ERA in 27 combined starts for Class A Bradenton and Double-A Altoona last year, allowed one run and two hits in two innings. The Pirates acquired him from the Dodgers in the three-team trade that Ramirez from Boston to Los Angeles and Jason Bay from Pittsburgh to the Red Sox in July 2008.


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Scripture… As We Live It #19
Here is the 19th edition of "Scripture… As We Live It":
These are blemishes on your love feasts, as they feast with you without fear, looking after themselves; waterless clouds, swept along by winds; fruitless trees in late autumn, twice dead, uprooted; wild waves of the sea, casting up the foam of their own shame; wandering stars, for whom the gloom of utter darkness has been reserved forever. Therefore, stop having love feasts altogether. If people are going to come in and spout heresy, then it is much better to cut out that kind of fellowship. Or, if you want to have fellowship and feasts, make sure that you tightly control who can speak. It is much easier to keep everyone from talking than to deal with the ones who cause trouble.(Jude 12-13 re-mix)
4 Comments
Comments are closed. If you would like to discuss this post, send an email to alan [at] alanknox [dot] net.
I think you may need to change the format of the remixes with your new theme. The light blue is hard to read on the white.
Oh I think you should keep it very light blue, maybe go little more aqua perhaps. Just in case people think that it is what it really says. Ugh. And if you keep tight control on everyone how do you discern those who just need to be pulled from the fire?
Love feasts and those messy dishes.
Just kidding about the light blue – it is a little too light.
Since you changed your font size for me, I suppose that I can change my font color for you. Seriously, thanks. I wrote these about a month ago, when the background was still dark blue.
Exactly… messy dishes and messy lives. We don't want either one as part of our church meetings, now do we?
So true! Didn't know you knew me thirty years ago! Can be very painful once ones eyes are opened!


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The Bulldozer Review: AMD FX-8150 Testedby Anand Lal Shimpi on October 12, 2011 1:27 AM EST
The Impact of Bulldozer's Pipeline
With a new branch prediction architecture and an unknown, but presumably significantly deeper pipline, I was eager to find out just how much of a burden AMD's quest for frequency had placed on Bulldozer. To do so I turned to the trusty N-Queens solver, now baked into the AIDA64 benchmark suite.
The N-Queens problem is simple. On an N x N chessboard, how do you place N queens so they cannot attack one another? Solving the problem is incredibly branch intensive, and as a result it serves as a great measure of the impact of a deeper pipeline.
The AIDA64 implementation of the N-Queens algorithm is heavily threaded, but I wanted to first get a look at single-core performance so I disabled all but a single integer/fp core on Bulldozer, as well as the competing processors. I also looked at constant frequency as well as turbo enabled speeds:
Unfortunately things don't look good. Even with turbo enabled, the 3.6GHz Bulldozer part needs another 25% higher frequency to equal a 3.6GHz Phenom II X4. Even a 3.3GHz Phenom II X6 does better here. Without being fully aware of the optimizations at work in AIDA64 I wouldn't put too much focus on Sandy Bridge's performance here, but Intel is widely known for focusing on branch prediction performance.
If we let the N-Queens benchmark scale to all available threads, the performance issues are easily masked by throwing more threads at the problem:
However it is quite clear that for single or lightly threaded operations that are branch heavy, Bulldozer will be in for a fight.
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TekDemon - Wednesday, October 12, 2011 - linkYeah I paid $179 for my i5 2500K and it hums along at 4.8Ghz (can hit 5Ghz+ but I wanted to keep the voltages reasonable). Clock for clock bulldozer is slower since it's only competitive when the higher clocked part is compared to a stock 2500K. Reply
jleach1 - Friday, October 21, 2011 - linkTheir cores offer, what 75% the speed of a normal core?
The fact is, this supposed "8" core processor performs worse than AMDs own 6 core processor. There's no way we can get away with calling it an 8 OR a 6 core.
For all intents and purposes, it's a quad core. Reply
estarkey7 - Wednesday, October 12, 2011 - linkYou took the words right out of my mouth! I am a big AMD fanboy, and I was waiting with baited breath to jump on the bulldozer bandwagon for my next rig (and I probably still will). But this is ridiculous! I'm a computer engineer and where the hell were the simulations AMD? Seems like you could have halved the L3 and kept in the extra FP resources and been better than what you are doing now.
Also, don't bitch about that Windows 7 doesn't realize the architecture of Bulldozer, you knew that 18 months ago, so you should have been writing a patch so that would have been a non issue.
The absolutely, positively only reason i will by an 8150-FX is that my current desktop is a dual core Athlon running at 2.2GHz. So to me, the performance increase over my current desktop would be massive. But on second thought, if I have stuck with such a slow system this long, I might another 3-5 months for Piledriver. Reply
Taft12 - Wednesday, October 12, 2011 - link<i>The power consumption is absolutely through the roof -- unacceptable for 32nm, really!</i>
Uhh, you did see the bar graph for idle power usage, right? And keep in mind this is an 8-core CPU compared to 4- and 6-core competitors.
Like you, I'm also very interested in the 4- and 6-core Bulldozers. Anand let us down by only reviewing the flagship Llano. Hopefully he doesn't do the same with Bulldozer. Reply
Tom Womack - Wednesday, October 12, 2011 - linkYes, the idle power is significantly worse than either of the Sandy Bridge platforms he's comparing it to Reply
JasperJanssen - Wednesday, October 12, 2011 - linkWhat Anand reviews is mostly down to what AMD will let him have -- even sites the size of Anandtech don't simply get to call and order parts from a catalogue for review samples. Reply
Taft12 - Wednesday, October 12, 2011 - linkAMD doesn't have much control over "review samples" that can be purchased at retail, as you can do with the A4-3300 et al. for weeks now Reply
enterco - Wednesday, October 12, 2011 - linkI read that 'at 1920x1200/1080 the gaming performance depends much mure on the GPU. Anyway, I'm happy with my i5-2500k ;-), Bulldozer does not seem to worth the wait. Reply
ninjaquick - Wednesday, October 12, 2011 - linkBlame shitty game developers. Reply
AssBall - Wednesday, October 12, 2011 - linkKinda what I was thinking. When they are all developing games for a 6 year old 3 core PowerPC system with 512MB RAM (xbox) instead of a computer, its no bloody wonder. Reply


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PlayStation Portable
Review by VergilDT
"An Amazing Piece Of Technology, Otherwise Ruined By The Number Of Defects"
1994. The Play Station X is released. 1996. The PlayStation is released. 2000. The PlayStation 2 is released. Now. Comes the God of all handhelds. The Sony PSP...
I just don't know what to say. This is easily one of the greatest things to happen to me. Sony has just taken my breath away...one day when you're getting old, you'll think back, nostalgic-like, and say "Wow. Just, wow". Sure we'll probably have 856765467-bit -::coughbitsdon'tmattercough:: -- graphics by then, but at least you know that the future of handhelds was started with the PSP.
Graphical Capabilites - 10/10 Wow. Just, wow. I never thought DreamCast-quality graphics were possible on a handheld! I thought handheld gaming would be forever stuck in the land of 2-D gaming. But no. Sony had to say "**** it", and went 3-D. But not like Nintendo. Sony had to make the resolution and texturing sexy as hell. The first time you turn on your PSP, you'll be "Wow. Just, wow." Oh, yeah, UMD movies look better than there DVD counter-parts on this thing.
Sound Capability - 10/10 Wow. Just, wow. I never thought CD-quality sound was possible on a handheld! I thought handheld gaming would be forever stick in the land of 32-bit sound. But -- ::Gets shot:: No really. The sound on this thing is amazing. Maybe that's the reason this baby is can have Atrac3 and MP3 files saved on a memory stick and played through the system. THe sound may sound unnoticeably...crusty when you're not using headphones or stereos, but it still sounds great.
Extra Features - 9/10 Nice, but not necessary. I don't really plan to cram all my music videos and movie trailors in there until I have at least a 256MB. The 32 is everything, game save/picture-wise, but absolutely crappy when it comes to videos and music, it sucks. So I would recommend dropping a few bucks for a bigger memory stick.
Durability and Defects - 4/10 Alright, now this is what brought the overall rating down. It's durability is decent, for the most part (won't break very easily, but scratches like a mother), but it's the defects that really bother me. I currently have a 15 dead pixel PSP in my room, but I'll still be playing. The dead pixels aren't really annoying, but just not acceptable. Sony really brings a pain in the ass, with their level of craftsmanship. I'm severely disappointed in how they handle things, and sometimes, can just be plain stupid ::coughplaystation-xcough::
Price - 7/10 $250 for a portable isn't really great, but is kind of reasonable, if you ask me. $40 for a game isn't too bad, at least. But a UMD movie price range of $13 - $30 is extremely idiotic, om Sony's part. Especially when you consider that there are no extra features. I would gladly pay $10 to $15 dollars for UMD music, but the movie price is stupid.
So overall...JUST BUY THE DAMN THING
Reviewer's Rating: 4.5 - Outstanding
Originally Posted: 04/25/05
Got Your Own Opinion?
Submit a review and let your voice be heard.


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I don't do a lot of movie reviews, although I would like to do more. I kinda wanted to review the Lone Ranger, because it bugged me so much that it could have been great. But it just wasn't.
Johnny Depp was great. He's a fantastic actor, and although he keeps playing Jack over and over, he does it really well. So the acting in the movie was really good at least.
The movie was way darker than I expected it to be and it should have been. It was a little over the top, the scene of the guy cutting out the other guy's heart and eating it comes to mind.
The action is the central part of the movie, or at least it was advertised that way. I actually got bored during the movie for quite a bit of time around the middle, which doesn't happen very often. I actually hardly ever get bored during a movie. Most of the action was pretty good, but there just wasn't enough stuff happening to make a movie of that length. On the other hand, the entire finale was fantastic. By far my favorite part of the movie. Those ten minutes (or however long it was) were amazing. But it wasn't enough to make the whole movie amazing.
I'm giving this movie a 6.9/10. It could have been really good, but it just wasn't there. If you've seen Lone Ranger, tell me what you thought!


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JACKSONVILLE, Fla. Winn-Dixie and the Jacksonville Jaguars have launched a co-branded bottled water that will be sold exclusively at Winn-Dixie and SaveRite stores.
"As the official grocery store of the Jaguars, Winn-Dixie is proud to provide football fans with access to exclusive products and programs that enhance the NFL game day experience," said Dan Portnoy, Winn-Dixie's chief marketing and merchandising officer. "We are excited to introduce this locally produced product and think it's an excellent way for Jaguars fans to show their team spirit – both in the stadium and around Jacksonville."
In line with the bottled-water launch, Winn-Dixie is offering its customers new promotions, just in time for football season:


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Jack and Irma Achmann celebrated their 50th wedding anniversary on Sept. 29, 2012.
They were married on Sept. 29, 1962 in Seven Dolors Church in Albany.
They have 4 wonderful daughters and fine sons-in-law.
Jack and Irma would like 40 or 50 more years of life and to thank God,
Jesus and Mary.


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I underwent a breast lift 3 years ago due to 60 lb weight loss. I am satisfied with getting perky, normal-looking breasts. My drooping was really bad, 3rd degree. I now feel I am lacking upper volume on my breasts, and while I do not want a LOT more size, I would like that round, sexy shape women get with implants. I think my nipples are too high now because if I wear anything with cleavage, they peek through (embarassing). I am 5'4 and 150 lbs. I am thinking a 250 cc implant might help. Any opinions?
Breast Implants 3 Years After Breast Lift for Upper Volume?
Doctor Answers 6
After Breast Lift Breast Implants Will Help Fill the Upper Pole of the Breast
Thank you for your question.
Yes, Breast Implants will give a previously lifted Breast more fullness of the upper pole or top of the breast.
See an experienced Board Certified Plastic Surgeons. If your breasts have "bottomed out" after your Breast Lift, the lower pole may need to be revised to push the Breast Gland and Implant higher.
Learn more: http://drseckel.com/breast-lift/sagging-breasts-how-to-lift-and-firm-your-breast-after-pregnancy/
Breast lift and implant for increased breast fullness
One of the biggest limitations with breast lift surgery is producing a fuller "upper pole" of the breast. A breast lift is produced by tightening the bottom of the breast, but this often improves the lower aspect of the breast, but not the top. As a result, I often recommend using an implant to "fill out" the upper half of the breast. Thus, there are possible solutions to help remedy your problems, but you should consult with a plastic surgeon in your area to discuss this in detail.
With respect to the nipples on the upper part of the breast, this is a difficult problem to correct. This can seem to occur if the breast has "bottomed out" and inappropriately descended below the fold. This can happen with large implants or in breast lifts where the scar has relaxed or stretched. Thus, there may a solution that can be addressed if you choose to have another surgery. Just "lowering" the nipple and areola will result in a visible scar where the nipple used to be.
Good luck.
Breast implant sizing
Much of the final "look" achieved after breast augmentation surgery depends on several factors:
1. The initial shape, size (volume of breast tissue), symmetry of the patient's breasts. In general, the better the preoperative breast appearance the more likely the breast augmentation "look" will be optimal.
2. The experience/skill level of the surgeon is important in determining the final outcome. For example, the accurate and gentle dissection of the breast implant pockets are critical in producing long-term well-placed breast implants. I personally think that these 2 factors are more important than any others, including type (saline or silicone) or model (low/moderate/high profile) of implant.
3. The type of implant used may determine the final outcome, especially if the patient does not have significant covering breast or adipose tissue. For example, some surgeons feel that silicone implants have a more natural look and feel than saline implants because silicone gel has a texture that is similar to breast tissue. Each patient differs in the amount of breast tissue that they have. If a patient has enough breast tissue to cover the implant, the final result will be similar when comparing saline implants versus silicone gel implants. If a patient has very low body fat and/or very little breast tissue, the silicone gel implants may provide a more "natural" result.
On the other hand, saline implants have some advantages over silicone implants. Silicone implant ruptures are harder to detect. When saline implants rupture, they deflate and the results are seen almost immediately. When silicone implants rupture, the breast often looks and feels the same because the silicone gel may leak into surrounding areas of the breast without a visible difference. Patients may need an MRI to diagnose a silicone gel rupture. Saline implants are also less expensive than the silicone gel implants.
Other differences involve how the breast implants are filled. Saline implants are filled after they're implanted, so saline implants require a smaller incision than prefilled silicone breast implants.
On May 10, 2000, the FDA granted approval of saline-filled breast implants manufactured by Mentor Corporation and McGhan Medical. To date, all other manufacturers' saline-filled breast implants are considered investigational. As of 2006, the FDA has approved the use of silicone gel implants manufactured by the Mentor Corporation and Allergan (formerly McGhan) for breast augmentation surgery for patients over the age of 22.
4. The size and model of breast implant used may make a significant difference in the final outcome. Therefore, it is very important to communicate your size goals with your surgeon. In my practice, the use of photographs of "goal" pictures (and breasts that are too big or too small) is very helpful. I have found that the use of words such as "natural" or "C cup" or "fake looking" means different things to different people and therefore prove unhelpful.
Also, as you know, cup size varies depending on who makes the bra; therefore, discussing desired cup size may also be inaccurate. I use intraoperative sizers and place the patient in the upright position to evaluate breast size. Use of these sizers also allow me to select the breast implant profile (low, moderate, moderate plus, high-profile) that would most likely achieve the patient's goals. The patient's goal pictures are hanging on the wall, and allow for direct comparison. I have found that this system is very helpful in improving the chances of achieving the patient's goals as consistently as possible. By the way, the most common regret after this operation, is "I wish I was bigger". I hope this helps.
Learn more: http://www.poustiplasticsurgery.com/Procedures/procedure_breastaugmentation.htm
You might also like...
The photos are difficult to look at, but the nipples do look like they are pointing upward. This may be because of how they were positioned at the time of your lift or from the implants bottoming out. Hard to say from the photos.
Learn more: http://www.stevenwallachmd.com
VIDEO (Click HERE) Breast Implants 3 Years After Breast Lift for Upper Volume
Your implants will likely settle in the lower portions of your breasts and only provide temporary upper pole fullness. You may want to consider fat grafting.
Learn more: http://www.bodysculptor.com/breast-surgery-chicago/breast-enlargement/
Breast implants after breast lift
Your question brings up a very common scenario. Patients frequently desire the sexy, rounder look that implants can provide when either combined with a breast lift or performed after a breast lift has been done previously. Additionally, you described your nipple position as being too high, commonly referred to as "star gazing nipples" which can certainly be seen after breast lifts or breast reductions especially over time as the tissue inferiorly tends to bottom out, especially with certain techniques.
Without actually seeing you, I would say that with an augmentation and probably a re-lift (excising some additional skin) you should be able to get the result you are looking for. Congrats on the weight loss, by the way.
Dr. Sean Simon
Miami, FL
These answers are for educational purposes and should not be relied upon as a substitute for medical advice you may receive from your physician. If you have a medical emergency, please call 911. These answers do not constitute or initiate a patient/doctor relationship.


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While revenues, membership and cost controls are all important to your club, one area that is often overlooked is the effectiveness of The Board.
Boards are the sole responsibility for the viability of clubs. Plain and simple. And getting the right people onto the Board is critical to success. In a perfect world, a Board would be composed of a diverse group of individuals who (among other things) share a strategic vision for the club, and who have the necessary skills, background, time and passion to perform their duties.
Unfortunately, that is not always possible.
Don't get me wrong. The majority of Boards out there do a stellar job. In a struggling economy, with other sports and pastimes competing for the almighty dollar, being responsible for a golf club's viability is not an easy task.
But at the same time, there are many board members in power who are simply not up to the task. This is not necessarily the fault of the board members themselves, but rather the fact that they may not have the necessary skills or time required, in the current environment, to thrive in their position.
A club is like a small-to-medium-sized business — often with huge turnovers, and potentially millions of dollars at stake. But not all clubs select their board members accordingly. While a large business, for example, has specific protocols in place to carefully select each member for their board, a golf club board is usually selected by popular vote at the AGM. And like most popularity-based voting processes (let's not comment on Federal Elections, shall we), people often get elected even if they aren't necessarily the best person for that position.
This is sometimes unavoidable, however, as it may be due to the limited pool of candidates.
A Club Board is usually composed of members from that club. A larger metropolitan club would have a pool of candidates from, say, large businesses or corporations. These candidates could be CEOs or Directors of huge businesses, with significant backgrounds in business management. But just because a person is a CEO of a company, for example, doesn't necessarily mean they know anything about marketing to women or junior golfers. And if they are successful in their business, will they necessarily have the time needed to devote to the club?
A smaller club, on the other hand, may not have the same pool of business-educated candidates. They may have a membership of blue-collar, small business owners. Being a successful small business owner does not necessarily mean they have the skills to direct a multi-million-dollar business. Nor do they often have the skill to professionally manage a committee (to reduce the red-tape and inanity of endless committee meetings that often dwell on minutiae). But what they may lack in Big Business experience, they more than make up for in passion and devotion to the Club.
So which is better?
Every club is different, as are the Boards that run them. There is no black or white answer to this…no one-size-fits-all board formula. Boards succeed (or fail) for many reasons.
But a key question to consider is: WHY are these people interested in the position?
A candidate will run for a board for any number of reasons. While many of these reasons are to improve the club, other reasons may be more self-serving. Do they have a truly strategic vision for the long-term future of the club, or do they just want their own reserved car parking space near the clubhouse? A new President, for example, may have an agenda to spend $200k to put in bunkers, simply because in his/her sole opinion it will make for a better look. But what the club may REALLY need is that $200k put into infrastructure, like better irrigation or drainage. While this is not necessarily a "sexy" spend of the money, it could potentially have a better long-term outcome for the club.
Many people that I've spoken to believe that a Board must be able to balance the needs of members with the financials, and should be composed of people that have a mix of backgrounds, age groups, golf skills, etc.
Aussie golfer Jack Newton recently said that he believes golf club boards and committees should be a diverse group.
"I have suggested the structure of a board should feature different age groups so that you get a perspective right across the board. There should be board members in their 20s, 30s, 40s…," he said at a recent industry forum. "At least with the young age groups you are going to get a perspective of where they see things are at rather than how older blokes see it."
No matter how you look at it, a Board is a critical part of any club. And at the end of the day, it may be time to either examine how we populate the boards, or how we can up-skill them to better prepare them to run in today's environment.
What do you think?
Richard Fellner
We want feedback from club members, Board members or anyone else wishing to share an opinion. What do you know about your current board members? Does your club have a successful process to select board members to ensure your club continues to grow? Is your board more successful than others? Why or why not? Should it be more or less corporate? Should Boards get remuneration?
Send us your emails and let us know. Confidentiality will be maintained should you wish to keep your name/club anonymous. But the more feedback we receive, the better.
5 Responses to "Getting your club on "board""
Right on the mark
"Your article should be the basis of a required reading paper for all prospective "board" members.
I worry about the introduction of the wording "board" member as against the previous "committee".
A lid needs to be kept on capabilities against responsibilities.
Some clubs have the good fortune of corporate leaders to call on that make themselves available.
Most, however, are made up of good meaning, caring "committee members" at best. I say that with the greatest of respect.
Too many of our clubs have gone under or are currently in bad shape, due to generally good committee intentions but bad decisions on matters totally out of their depth.
Your points:
1. Careful, best qualified selection vs popular vote. You're right.
2. New C'tee/board members making their mark with change for the sake of change. Right again.
Resisting change until every box is ticked is the required skill.
Course/Building/Major financial decisions should not be implemented without referral to and advice back from qualified respective professionals.
You finished posing the question "How we can up-skill them to better prepare them to run in today's environment…". Clubs are paying a lot of money in affiliation fees, insurance, magazines etc to Golf Australia and their State bodies. They would better serve their member clubs by providing business, club committee/ board guidance than catering to social golf clubs.
Doug Perry
Mt Martha. Vic.
If it ain't broke, fix it anyway
I am a board member of a Melbourne metropolitan club, and your article was so true in respect of the type of person that is required to be on the board. We have a successful gaming venue which heavily subsidizes the golfing activities and, as such, as far as the members are concerned money is no object. The board is basically comprised of retired persons with little or no business experience, yet we are running a business with a turnover in excess of $5.0m and I am not sure how we get change. The attitude is "if it ain't broke, why fix it", instead of trying to improve and getting more professional!
Jack Newton is right about a board being diversified but it really is a question of people being willing to put their hands up for a job, however in most instances it is a closed shop with people not wanting change.
I have a board meeting this week and I will provide the article to my fellow members.
Alan Jones
Board killing the club
I will briefly outline the profound mismanagement by recent Boards at our Golf Club. Over the past ten years, successive Boards have:
• Imposed an $8m debt on our Club. (Huge mortgages whilst repaying interest only).
• Placed the Club in prima facie insolvency with current liabilities $1,542,050 exceeding assets of $385,045.
• Posted substantial losses over the past two years – 2011/$458,616 and 2010/$473,148.
Given that our Beachside Club is the jewel in this Tourist District crown, this situation is inexplicable. I am told that there is no remedy to be found against the persons responsible for this debacle. The future of many of our Club facilities is now in great jeopardy.
The above situation would not have occurred had we a CEO with total responsibility for business and golf operations. This would necessitate a change to the Club's constitution to give the CEO total control/veto over Board propositions/decisions. (The CEO position would need to be filled by utilizing the services of 'headhunting' professionals.)
Trev & Ray
Diversity is key
One the one hand, you have directors who try to do the best for their club and often have a thankless task. On the other hand, they have to get voted at an AGM, and often few people want to do the job.
So the majority of board directors often end up being aged 50+. They have a lot of knowledge of the game, but usually have limited foresight due to a number of factors:
– usually play more than 90% of their golf at their home club
– they don't see what is going on with junior golf
– didn't grow up in the computer age, so don't envisage technology
– find change difficult
Jack Newton's comments that golf board's should be made up of a range of age groups is the way I believe boards should be. There would then be a mix of golf experience, current ideas and capacity to change.
Golf has a major problem now in that there is a lack of players in the 20-40 age group. In the 80's, golf rode off the Greg Norman boom, but if you look in Sydney for example, all club memberships have been reducing for a number of years. Yet, there's been very little changes in attitudes of golf clubs to fostering juniors, ladies competitions and membership generally.
As with directorship generally, there are often disputes in the group, and too often I have seen golf board disputes lead to favouritism in regard to team selections as some board members enjoy the power their role gives them.
To be an effective board member you need to listen to members' concerns, and be able to voice the board's view in a rational manner, as well as have the foresight and vision to advance the club. A business plan can be an effective tool in growing a business, yet I know of few clubs who can foresee more than 12 months in advance.
Golf boards need to move with the times, otherwise all clubs will be struggling with ageing memberships. A mixture of different age groups on board's is definitely an answer, which will probably mean that some potential board members will have to be chased rather than voted for at an AGM.
Experience, not popularity
I was the Finance Director of a Golf Club in S.E. Melbourne for seven years. I have an accounting background which I found to be important in trying to get the financial message across to fellow Directors and members.
Being a Director is a very time consuming position and requires a close working relationship with the GM and other committees for the Board to function efficiently. This is especially so with the Finance Director.
There is no benefit in being a monthly attendee at Board meetings if that is the extent of your participation and input.
Your comment on people often getting elected for the wrong reasons is very true. Popularity polls do not necessarily give you the right person for the job especially where there are supporting factions within the Club for particular candidates.
You do find that some people want to get on the Board to push their own agenda and don't balance the needs of Members with the financial requirements of the Club. They want to protect their self interests and don't want to change what has been the practice for years.
Getting people in the 30+ age bracket to become members is very difficult in this economic climate let alone committing to being a Board member. The trend for this age bracket seem to be more focused in getting together with a few mates and playing different courses once a fortnight or month, and especially so if there are family commitments.
John Miller


text:
Stem Cells International
Volume 2012 (2012), Article ID 167265, 10 pages
The Flatworm Macrostomum lignano Is a Powerful Model Organism for Ion Channel and Stem Cell Research
1Hubrecht Institute, KNAW, University Medical Center Utrecht, 3584 CT Utrecht, The Netherlands
2Institute of Cytology and Genetics SB RAS, 630090 Novosibirsk, Russia
3European Research Institute for the Biology of Ageing and University Medical Center Groningen, University of Groningen, 9713 AV Groningen, The Netherlands
Received 27 April 2012; Accepted 2 August 2012
Academic Editor: Michael Levin
Copyright © 2012 Daniil Simanov et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Bioelectrical signals generated by ion channels play crucial roles in many cellular processes in both excitable and nonexcitable cells. Some ion channels are directly implemented in chemical signaling pathways, the others are involved in regulation of cytoplasmic or vesicular ion concentrations, pH, cell volume, and membrane potentials. Together with ion transporters and gap junction complexes, ion channels form steady-state voltage gradients across the cell membranes in nonexcitable cells. These membrane potentials are involved in regulation of such processes as migration guidance, cell proliferation, and body axis patterning during development and regeneration. While the importance of membrane potential in stem cell maintenance, proliferation, and differentiation is evident, the mechanisms of this bioelectric control of stem cell activity are still not well understood, and the role of specific ion channels in these processes remains unclear. Here we introduce the flatworm Macrostomum lignano as a versatile model organism for addressing these topics. We discuss biological and experimental properties of M. lignano, provide an overview of the recently developed experimental tools for this animal model, and demonstrate how manipulation of membrane potential influences regeneration in M. lignano.
1. Introduction
Ion channels represent a diverse family of pore-forming proteins. They are crucial for establishing voltage gradients across plasma membranes by allowing the flow of inorganic ions (such as Na+, K+, Ca2+, or Cl−) down their electrochemical gradients. Ionic flux through the channels provides the foundation for membrane excitability, which is essential for the proper functioning of neurons, cardiac, and muscle cells [1]. At the same time, ion channels serve many functions apart from electrical signal transduction. For example, Ca2+ is an important messenger, and changes in its intracellular concentrations influence numerous cellular processes in virtually all types of nonexcitable cells [2–4], including stem cells [5–7]. Besides, a number of ion channels are known to be directly involved in chemical signaling pathways in different cell types [8, 9]. As a result, mutations in genes encoding ion channel proteins have been associated with many disorders (so-called "channelopathies"), caused by dysfunction of both excitable (epilepsy, hypertension, cardiac arrhythmia) and nonexcitable (diabetes, osteopetrosis, and cystic fibrosis) cells [10]. Here we briefly describe the crucial role ion channels play in maintenance, proliferation, and differentiation of stem cells on the level of single cell and the whole organism. We discuss the importance of animal model systems, such as flatworms, for studying bioelectric signaling in complex morphogenesis during development and regeneration. Finally, we introduce the new flatworm model, Macrostomum lignano, and discuss its experimental potential for dissecting the roles of ion channels in stem cell regulation.
2. Ion Channels and Membrane Potential in Stem Cells
Numerous ion channels and pumps together with gap junction complexes form transmembrane voltage gradients. While quick changes of these membrane potentials () are best described in neurons, muscle, and cardiac cells, long-term steady-state levels are present in all other cells [11, 12]. Membrane potentials strongly correlate with the mitotic ability of different cell types, with the high resting potential associated with differentiated nondividing cells [13]. fluctuations during progression through the cell cycle have been reported in a number of cell types, and changes of membrane potential appear to be required for both G1/S and G2/S phase transitions [14–16]. Modulation of through applied electric fields or by inhibition of ion channels leads to cell cycle arrest in dividing cells [17–20], and artificial membrane hyperpolarization induces differentiation of mesenchymal stem cells [21]. On the other hand, electroporation (supposedly followed by membrane depolarization) activates cell hyperproliferation and de-differentiation [22].
On the level of multicellular organism, progression through the cell cycle should be strictly regulated and synchronized during such processes as development and regeneration in order to achieve a proper body patterning. Accordingly, stable and reproducible membrane polarization patterns have been recently described in various model organisms. Artificial modulation of these patterns during development or regeneration has a large impact on left-right asymmetry and anterior-posterior identity [23–27]. The role of bioelectric signaling in regeneration is comprehensively reviewed in [28] and schematically shown in Figure 1. Finally, modulations of membrane voltage have been observed in a large number of oncological disorders, and ion channels were proposed as cancer treatment targets [29, 30].
Thus, bioelectric signaling is an important mechanism of cell regulation, including stem cell maintenance, proliferation and differentiation. Recent findings suggest this control system to be well conserved in a wide range of animal phyla. However, the mechanisms linking membrane potential to the cell cycle, proliferation and differentiation, and the role of specific ion channels in this process remain largely unclear. The picture becomes even more complicated on the level of multicellular organism. Our understanding of the ways cells produce and receive bioelectric signals and translate them into positional information during development and regeneration is still fairly poor. While considerable knowledge about the role of membrane potential in stem cells was gathered recently from different species, the number of models used in this field is still limited. Expanding the range of model organisms used for functional studies of bioelectric signaling is crucial for better understanding of this control system and its role in complex morphogenesis.
3. Planarian Models in Ion Channel Research
Planarian flatworms are long-established models for stem cell and regeneration research. The adult stem cell system and regeneration capacity of the species Planaria maculata and Planaria lugubris were described by Morgan as early as in the end of 19th century [31, 32]. In our days the favorite planarian species for research in the regeneration field are Schmidtea mediterranea and Dugesia japonica [33, 34]. Planaria were also one of the first species in which stable membrane potential patterns were described, and their role in regeneration postulated. In 1940s and 1950s Marsh and Beams were able to specifically control establishing of anterior-posterior axis by providing bioelectrical signals to regenerating planaria fragments [35–37].
In the last 5 years considerable work was done in planaria on understanding the molecular and genetic mechanisms that allow cells to establish and maintain long-term membrane potential patterns and transduce bioelectric signals into proliferation and differentiation decisions. The importance of gap junction signaling in establishing anterior-posterior polarity during regeneration was shown [38], and the specific innexin gene, Smedinx-11, responsible for blastema (regenerating tissue) formation and stem cell maintenance identified [39].
The role of ion channels and pumps in the establishment of anterior-posterior axis during regeneration of planaria D. japonica was recently highlighted by groups of Michael Levin and Jonathan Marchant. D. japonica, which can regenerate an entire animal from a small part of a cut worm, has highly depolarized cell membranes in the head region, and highly polarized in the posterior part. In the cut worm this pattern is reestablished rapidly, regardless of the cutting plane [26]. After the wound is closed, blastema at all anterior-facing wounds gives origin to heads, while tails are regenerated from the posterior-facing wounds. The polarization pattern is altered by highly specific drugs against different ion channels and transporters, such as SCH-28080 (inhibitor of H+, K+-ATPase), ivermectin (IVM, activator of the invertebrate GluCl channels), or praziquantel (PZQ, activator of voltage-operated Ca2+-channels). Remarkably, induced depolarization itself is sufficient to drive ectopic anterior (head) regeneration even in posterior-facing blastemas, whereas membrane polarization of anterior-facing wounds blocks the head regeneration [25, 26]. The role of specific voltage-operated Ca2+ channels in regenerative patterning was addressed in the followup experiments [27].
Thus, planarian flatworms can be successfully used for ion channel and stem cell studies. Fascinating regeneration capacity of these animals, together with a wide range of research techniques established and optimized over the last 100 years, make planaria a very attractive model for studying bioelectric signaling during regenerative morphogenesis. However, due to inefficient sexual reproduction under laboratory conditions, classical genetic methods are not available in planarians, and reverse genetics methods are limited to RNA interference. Since genetic manipulation of these animals is difficult, no reproducible transgenesis methods are available for planaria [40].
4. Experimental Properties of the Flatworm Macrostomum lignano
During the last decade another flatworm, Macrostomum lignano, has emerged as a complementary model organism for regeneration research [41–44]. This marine free-living basal flatworm is about 1.5 mm long and consists of roughly 25000 cells. M. lignano is easy to culture in laboratory conditions, and populations of this animal are continuously maintained in the number of laboratories for over a decade. The generation time of the flatworm is short, with about two weeks of postembryonic development to sexually mature adult. Both juvenile and adult worms have clear morphology and are highly transparent (Figure 2(a)), greatly facilitating phenotyping and both fluorescent and non-fluorescent staining. The regeneration capacity of M. lignano is provided by roughly 1600 neoblasts (adult stem cells) located mesodermally. Proliferation activity of these cells can be easily studied using BrdU labeling, performed by simple soaking [43, 45] (Figure 2(b)). Importantly, M. lignano is nonself fertilizing hermaphrodite and has exclusively sexual reproduction. Well-fed adult animals generate a lot of embryos all year through (one animal lies on average one egg a day), making it accessible for genetic manipulation. In situ hybridization [43] and RNA interference (by soaking) [45] protocols are established and optimized for M. lignano, and a number of tissue-specific monoclonal antibodies are available [41]. Basic culturing and experimental properties of M. lignano are summarized in Table 1.
Considerable progress has been made in the past three years towards establishing M. lignano as versatile stem cell research model for the genomics era. The work on M. lignano genome assembly and annotation is in progress (Berezikov and colleagues), and draft genome and transcriptome assemblies are publicly available at http://www.macgenome.org/. Comparing transcriptome data obtained from irradiated (neoblast-depleted) and control worms provided the insight into the role of a number of genes in regeneration, while stage-specific transcriptome data showed the temporal expression of Macrostomum genes through development (Simanov et al., in preparation). Most importantly, proof-of-principle for transgenesis in M. lignano has been demonstrated and first stable transgenic GFP-expressing lines of M. lignano have been established (Demircan, De Mulder, Berezikov et al., in preparation). Thus, biological and experimental properties of M. lignano, combined with its rapidly expanding experimental toolbox, make this animal an attractive and powerful model organism for stem cell and regeneration research. Its astonishing ability to resist γ-irradiation and recover after being exposed to it makes the neoblast system of this animal exceptional even for flatworms [46]. Moreover, fascinating but yet poorly understood link between regeneration and rejuvenation provides an exciting opportunity of using M. lignano as a model for ageing research [47].
5. Ion Channels and Regeneration in M. lignano
Unlike planarian flatworm species, M. lignano is unable to regenerate the head under normal circumstances. Posterior-facing blastemas give origin to fully functioning tails with all its organs and structures, whereas anterior-facing wounds develop blastema layer but the actual regeneration can only happen if the worm was amputated in front of the brain (at the very tip of the head). Thus, anterior fragments of the worm, having a functional head, can regenerate the whole body in 2-3 weeks, while posterior fragments normally die 5–10 days after losing the head [42]. These differences in the head regeneration capacity between M. lignano and planarians, and the ability to induce ectopic head regeneration in D. japonica by the manipulation of membrane voltage gradients, prompted us to investigate how these findings in planarians translate into M. lignano.
DiBAC4(3) voltage-reporting dye stainings (as described in [48]) showed that membrane voltage pattern in M. lignano is similar to the one observed in D. japonica [26]—the anterior part is highly depolarized, while the tail is relatively polarized. In the cut worms this pattern is quickly reestablished in the anterior head-containing fragments, while the posterior headless fragments do not show any clear anterior-posterior polarization gradient and do not regenerate (Figure 3(a)). Just like in planarians, membrane polarization patterns in M. lignano can be altered using drugs against ion channels. IVM induces depolarization of the membranes of intact and cut worms, both in anterior and posterior regions (Figure 3(b)). Posterior-facing blastemas still regenerate the tails after treatment, though the full regeneration takes longer than normally. Anterior-facing wounds treated with IVM develop blastema, and some tissue growth is often observed within a week after wound closure. IVM-treated headless fragments always move more actively and survive longer comparing to control fragments. Strikingly, 1.5% of posterior fragments after IVM treatment are able to regenerate head-specific structures and, in a few cases, a fully functional head (Figures 3(c) and 3(c′)). PZQ causes the same depolarization effect but does not have any effect on regeneration patterning at tested concentrations (data not shown). Intact animals exposed to high doses of IVM or PZQ display phenotypes that in planarian flatworms are stereotypically associated with stem cell loss or disorder [49–52]. M. lignano animals treated with 2 μM IVM gradually lose anterior identity, with no head-specific structures left 7–9 days after treatment (Figure 3(d)). After exposure to higher doses of IVM (3-4 μM), worms develop characteristic square head due to partial tissue loss in the most anterior part of the body, get paralyzed and die 3-4 days after treatment (Figure 3(d′)). High concentration of PZQ in culturing media causes formation of bulges, mainly in the posterior part of the body (Figure 3(d′′)). This phenotype is completely different from the one observed after IVM treatment, suggesting specific action of the drugs.
These pilot experimental results show that M. lignano can be successfully used as a model for ion channel and stem cell studies. The complete transcriptome and established in situ hybridization and RNA interference methods, in combination with chemical treatment make it possible to address the function of specific ion channels in development, tissue turnover, and regeneration. For example, comparison of transcriptome data from irradiated (stem cell-deficient) and nonirradiated animals (Simanov et al., in preparation) highlights a number of ion channel genes expressed specifically in dividing cells (Figure 3(e)), and future elaborated studies of such genes may provide novel insights into the role of bioelectric signaling in stem cell maintenance and differentiation. Importantly, a significant number of ion channels are well-conserved between M. lignano and human (Table 2), increasing the relevance of findings in flatworms to understanding ion channels and stem cells in human situation.
6. Future Directions
We advocate that Macrostomum lignano has great potential as a model for ion channel and stem cell research. The genetic toolbox available for this organism is already useful enough to address a wide range of scientific problems, and more methods and approaches will be optimized and used in this flatworm in the near future. M. lignano is a small animal and it is cultured in water, which makes it easy to apply different chemicals to the worms. Another major advantage of the animal is its high transparency. Phenotypic changes, fluorescent signals or certain transgene expression can be observed in any part of the body, as well is on the whole organism scale. For example, various fluorescent reporter dyes can be just added to culturing media in order to enable real-time in vivo monitoring of membrane potentials, pH, and ion flows [53]. Short generation time and efficient reproduction of M. lignano make logistics of large-scale experiments, such as drug screens, feasible in this animal.
As a model, M. lignano offers an exciting opportunity to bridge the gap between bioelectric signaling and genetic pathways involved in stem cell functions. The expression pattern and function of any gene can be determined by in situ hybridization and RNAi protocols, but it is transgenics that can bring such studies to the whole new level. Transgenic reporter lines expressing pH-sensitive or Ca2+-sensitive fluorescent proteins [54, 55] would make a perfect tool to visualize bioelectric phenotypes during drug- or RNAi-screens. Overexpression of ion channels or even certain subunits would help to better understand their functions and interactions. Targeted genome editing by Zinc Finger Nucleases have not been tested yet in this animal but should be also feasible and potentially can be used to generate ion channel knockout and knock-in lines [56, 57]. The same method allows fluorescent tagging of genes of interest and analysis of their expression, localization, and functions at the endogenous level [58]. Sexual reproduction and lack of self-fertilization make possible crossing different lines of M. lignano and hence to use the power of classical genetics approaches in this animal. Taken all together, we are convinced that M. lignano is poised to become a productive model to study relations between ion channels and stem cell regulation (Figure 4).
The authors thank S. Mouton and T. Demircan for critical comments on the paper, and S. Mouton for providing the image of the BrdU-labeled M. lignano animal.
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It will be quite the award season for Angelina Jolie.
The star of A Mighty Heart is nominated for a Golden Globe and, it was announced this morning, a Screen Actors Guild Award.
For best actress, Jolie will vie with: Cate Blanchett (Elizabeth: The Golden Age), Ellen Page (Juno), Julie Christie (Away From Her) and Marion Cotillard (La Vie En Rose).
Nominated for best actor are George Clooney (Michael Clayton), Ryan Gosling (Lars and the Real Girl), Daniel Day-Lewis (There Will Be Blood) and Viggo Mortensen (Eastern Promises).
For the complete list, click here.
The awards will be telecast on TNT and TBS on January 27 despite the writers' strike, thanks to a deal between the Actors Guild and the Writers Guild of America for one of its members to write the ceremony.
Tell Us: Who deserves to win?


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Here's some good news: Madden NFL 11 for iPad, the #1 NFL experience in the video game industry, has been hit with a price drop! it was once $12.99, but now you can pick it up for just $6.99 in the App Store
If you haven't given it a try yet, now's the time. The touchscreen controls are intuitive, the graphics fantastic and HD-quality, Vintage Voltage Football is a fun spin on a classic sport, and you can even take on friends via your Bluetooth connection. Visit the App Store and take it for a spin.


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In this video, I share a case study with you where I actually got people to sponsor my business projects and I also show you how you can do it in three easy steps.
If you like this video, please consider joining my 48 Days Internet Marketing Group for new updates.
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Zhen Liu
Zhen Liu was born in 1970. He obtained his Ph.D. degree from the Dalian Institute of Chemical Physics (DICP), Chinese Academy of Sciences (CAS), China, in 1998. From February 2000 to January 2002, he performed his first postdoctoral research in the University of Hyogo (formerly the Himeji Institute of Technology), Japan. From August 2002 to November 2005, he performed his second postdoctoral research in the University of Waterloo, Canada. From December 2005, he was appointed as a Full Professor at the School of Chemistry and Chemical Engineering, Nanjing University. He is currently an Adjunct Professor at the Department of Mechanical and Mechatronics Engineering, University of Waterloo. He is currently a Member of the Editorial Board of the Chinese Journal of Chromatography (Sepu) and ISRN Chromatography. He has authored and coauthored 1 monograph and 87 peer-reviewed papers, which have obtained citations more than 1200 times (h index: 22). He has delivered more than 20 invited and plenary lectures in international and domestic conferences and institutions. His research interest is mainly the development of novel functionalized materials and hyphenated approaches for the separation and analysis of biomolecules.
Biography Updated on 4 December 2012


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We are continually reminded that in most areas of life, effective communication is the key to understanding and harmony. As we consider the church family we see that the reporting of activities and their results is a vital part of internal communication. In many churches there is much activity, but only those involved in each … [Click to read more …]
Category Archives: 2012b Evangelism and Witnessing
As soon as someone mentions reports, you may imagine reams of paper filled with facts and statistics that will probably do little more than gather dust. However, reporting is not a modern invention designed to frustrate those involved in witnessing and evangelism. It is a biblical principle. As our memory text for this week reveals, … [Click to read more …]
Read for This Week's Study: Acts 4:1–31, 21:19–25, 1 Cor. 9:19–23, Num. 13:17–33, Acts 11:1–18. Memory Text: "Then the apostles gathered to Jesus and told Him all things, both what they had done and what they had taught" (Mark 6:30, NKJV). Key Thought: As a report of the early church's missionary endeavors, the book of Acts is filled with lessons … [Click to read more …]
Does the past ever haunt you? Do you ever wish you could go back and do something differently? Every baseball season I am haunted by something that I did, or actually didn't do when I was 12. Many springs ago, I was a little league baseball player. It was my first year of organized baseball, … [Click to read more …]
Keeping on Track Just as any good motor vehicle will ultimately break down as a result of a lack of regular maintenance, so many good and worthwhile church ministries have fallen by the wayside because of a lack of regular intentional maintenance. To keep your ministry healthy and on track, consider the following maintenance check … [Click to read more …]
Melody and Stewart Part 2 "Good morning, class," Stewart said after the general greetings and opening prayer had been shared. "We're going to do something today we've never done before." He looked around. "Let's start with Matthew 18:19 and 20." Bible pages rustled as class members turned to the required text. "Go therefore and make … [Click to read more …]
The Bible makes it clear that we were once slaves to sin, but through Christ we have been released (Rom. 6:6), set free (Gal. 5:1), delivered (1 Thess. 1:10), adopted (Rom. 8:15), and born again (1 Pet. 1:23). The effective worker for God is the one who has given the past to Him and has accepted His power … [Click to read more …]
There's an English expression that says: "There is no such thing as a free lunch"–the idea that if you receive something free, it really isn't free because somewhere, somehow, sometime, you will have to pay or repay. The theory that nothing is ever really free has subtly infiltrated Christian thinking to the extent that many … [Click to read more …]
Key Thought : Christ's loving service to us should awaken a loving response as the basis for our witness as reflected in our loving ministry to others. [Teaching plan for A Love Response June 4, 2012] 1. Have a volunteer read I John 4:18,19. A. Ask class members to share a short thought on … [Click to read more …]
What would you think of someone who constantly and loudly declared that they were motivated and yet attempted nothing? What about someone who claimed to be dedicated yet never revealed to what, or to whom, they were dedicated? As we have seen, love is a most powerful motivator; but to only declare our love, even … [Click to read more …]


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Now that you know everything there is to know about Apple TV, here's a service where you can pay someone to upgrade it for you. Sure, you may be comfortable swapping out the disk, but your mom or uncle—who the Apple TV is targeted to anyway—may not.
To upgrade the 40GB disk to 60GB costs $195, and the highest it goes is 160GB for $295. Most of that is labor, but you're familiar with that concept with mechanics and doctors anyway.
Product Page [MacService via MacWorld]


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While the games at the annual East Coast Invitational team basketball camp are simply summer exhibition contests, they can play a vital role in some team�s progress as it looks forward to the upcoming season.
While the games at the annual East Coast Invitational team basketball camp are simply summer exhibition contests, they can play a vital role in some team�s progress as it looks forward to the upcoming season.
Just ask Northside High coach Tony Marshburn, whose squad became the first Onslow County team to capture the ECI championship last year.
�It gave us a ton of confidence,� he said. �In the East Coast you have to beat very good teams.�
The Monarchs certainly did that last year en route to the ECI title, a feat that Marshburn said helped Northside later win the East Central 2-A Conference tournament title and advance to the NCHSAA East Regional final for the first time in program history.
The Monarchs finished 23-4 overall, falling to Kinston 69-58 in the regional final in Fayetteville. Kinston, which had lost to Northside in the ECI, went on to win its third consecutive state championship.
�It was a fun run and obviously beating Kinston (in the ECI) was a highlight because year in and year out Kinston is one of the teams picked to win a state championship and they will be another favorite again to win it,� Marshburn said. �We are going to have to play well to win.�
And while Marshburn would like to see his Monarchs seize another ECI title, he understands winning games in the summer isn�t the most important thing. Rather, he wants to see improvement during the dog days of summer and he wants to get a sense of which players will be ready come the regular season.
The ECI begins Thursday and concludes Sunday � games are played at the Jacksonville Commons Recreation Center, Northside High School and Jacksonville Commons Middle School. The Monarchs open with Jack Britt.
Northside is joined by White Oak, Jacksonville, Southwest and Dixon as area teams playing in the ECI. White Oak finished 14-12 overall last year and advanced to the state 3-A playoffs. Jacksonville ended 13-12 and was playoff-bound in its first season under coach Doug Erny and Southwest finished 19-4, won the ECC regular season title and advanced to the East Regional semifinal, falling 54-44 to Northside.
Dixon finished 11-15, but is expected to return 12 players. The 24-team field also features Kinston and reigning state 1-A runner-up East Carteret.
Kinston is led by 6-foot-9 standout Brandon Ingram, who has received offers from Duke, North Carolina, N.C. State, Wake Forest, Louisville, Kentucky, Kansas, Florida and UCLA, among others.
�First and foremost we want to see how we stack up against really good competition,� Marshburn said. �I know all of the coaches say the same thing in that you want to see if you can defend and execute a play against quality competition, and you want to see how hard the kids play. It�s a tough tournament and obviously there�s good teams in it so you have to play at a high level because you�re trying to find out how you team can play at that level.�
Marshburn said he recently spoke to his team about its quest to maintain a high level and that he�s looking for his returning players about trying to push the new ones to �perform at a higher level,� this summer and during the regular season.
The Monarchs are led by the Curry brothers � seniors Kris and Korey � and sophomore Zach Hobbs. Northside graduated six players, including The Daily News player of the year Davion Ayabarreno and Josh Cabrera, who will play football at the University of North Carolina this fall.
Overall, Marshburn has been pleased with the effort this summer by the Monarchs.
�I�ve seen some good things out of this group with one of the biggest things has been their unselfishness,� he said. �We played in one tournament this summer and I thought we shared the ball well.�


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3yrs Breakdown Care TV 150 to 199.99890/0621
About this product
What would you do if your washing machine broke down? Or if your TV went blank? Finding a reliable repairer could be a big hassle – and could be expensive too. If you add Argos Breakdown Care, provided by Domestic & General, you won't have to worry about who to call. From the moment you buy the plan, Argos Breakdown Care can help.
What's included:
- Protection against breakdowns and damage caused by accident.
- Repairs and replacements.
- Unlimited repair requests; if we can't repair it we will replace it.
- UK contact centre.
- National network of qualified engineers.
What's not included:
- Deliberate damage.
- Delivery and installation charges where applicable.
- Cosmetic damage that doesn't stop the product from working.
General information:
For the full list of exclusions and restrictions, please see the terms and conditions available online, or in a leaflet in Argos stores. Your Statutory Rights will not be affected when you buy a plan. These include the rights to claim a refund, repair or replacement for up to 6 years (may be different in Scotland) if your electrical goods were not of satisfactory quality or fit for purpose when they were sold to you.
You can buy a plan at the point of purchase or within 45 days of buying your item. Your plan can be cancelled at any time within 45 days of purchase. You will receive a full refund if u have not used any services under the plan. After the 45 day cancellation period you may terminate the plan at any time. If you have not received a replacement, we'll refund the fee paid by you for the remaining full months of your plan. If your item is replaced under the plan your plan will end.
The obligations under your Domestic and General plan are backed by assets held within a trust for your protection. Plans may be available from other providers. You may also be covered under your household contents insurance or other policies that you hold. You can compare the prices of product care for your electrical good online using any internet enabled device at www. Compareextendedwarranties. Co. Uk.
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*Prices correct as displayed but are subject to change.
You can compare the prices of product care for your electrical goods online using any internet enabled device at www.compareextendedwarranties.co.uk.


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Family vacation destinations: Lilium - Wallpaper size 1600x1200 free download
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Beautiful wallpaper images free download: You could download and use these pictures absolutely freely as wallpapers for your computer, tablet or smartphone. Fine art photography - Extending the limits of the soul. Copyright © 2015 Dina Shvayakova & Andrew Karpov. All rights reserved.
Free wallpapers and photos are made available for your personal, non-commercial use only. You may not distribute, modify, transmit or use the content of this site for public or commercial purposes without site owner's expressed written permission.
Images on the website have been digitally watermarked with ownership and usage information. Digimarc and the Digimarc logo are registered trademarks of Digimarc Corporation. The "Digimarc-Enabled" Web Button is a trademark of Digimarc Corporation, used with permission.
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Whilst every effort has been made to ensure that the information on this site is as accurate as possible, the authors cannot be held responsible for any inaccuracies or consequences arising out of the use of this site.


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Local country singer Betty Rydell and her granddaughter Randi Rae will present a concert at the Coon Rapids Dam Regional Park performance pavilion Thursday, Aug. 2, 7 p.m.
The free outdoor show is part of Coon Rapids Arts Commission's 2012 Thursday Nites Live summer series – the eighth of nine concerts.
Rydell has entertained with not only country, but gospel, patriotic and variety music for many years playing nine musical instruments.
She has performed all over the United States, Canada, Europe and Central America and has appeared on stage with Barbara Mandrell, Charlie Pride and Conway Twitty, among others.
Rydell is a winner of the Entertainer of the Year Award presented by the Country Entertainers Association of the Upper Midwest.
Rydell and Rae, who has been performing country songs for many years herself, will both be appearing at the 2012 Minnesota State Fair Thursday and Friday, Aug. 23 and 24, giving three performances each day.
The remaining concert on the 2012 schedule is Thursday, Aug. 9 when the Sonny Winberg Band will entertain with top 40 country music.
The Shortstop Bar & Grill on Northdale Boulevard is sponsoring the Aug. 2 concert, one of eight in the 2012 series that it is sponsoring.
For refreshments at the performance pavilion, Boy Scout Troop 524, Church of the Epiphany, is selling popcorn, candy, soda and water, while the Coon Rapids North Star Lions Club has food items such as hamburgers, cheeseburgers, hot dogs, walking tacos, nachos and cheese and brats for sale.
The Coon Rapids Arts Commission, which is a volunteer advisory commission to the city council, plans arts events in Coon Rapids throughout the year.
The Anoka County Parks and Recreation Department is co-sponsoring the series. It is not charging admission to the regional park for the concerts.
For more information call 763-767-6432 or 763-757-4700.
Peter Bodley is at [email protected]


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Seven Indiana schools awarded grant money
Seven Indiana schools will share more than $8.5 million in federal grants, State Superintendent Glenda Ritz announced Thursday.
The School Improvement Grants are awarded to schools that show a need, along with a commitment to use the funds to raise achievement levels. The grant money will be awarded over the next three years.
Stonybrook Middle School in Warren Township is the lone Indianapolis-area school to receive grant funding and will receive the most money of the seven schools ($1.85M). Fairview and Highland Park elementary schools in Monroe County and McCulloch Middle School in Marion are the other central Indiana schools on the list.
"I am honored to award these grants today," Ritz said. "My department is committed to providing schools and communities with the resources they need to ensure that all Hoosier children receive an equitable and high quality education. These schools have demonstrated a great need for these funds, as well as excellent plans to improve student performance."
More on School Improvement Grants


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On Mon, 22 Dec 2003, Joshua D. Drake wrote: > > >so the problem is in the sgml itself, and not the tools... ? > > > > > > > > Well, I have yet to get that far but I am running SuSe 8.2 and Fedora. I > can duplicate > the problem on both. > > I would suspect that something in the SGML is causing it to loop on > itself. It may be > a bug with openjade that is rearing its head because of something we are > doing in > the docs. > > I am going to dig a little more and see if I can find the exact problem. 'k, on the dev server, I get a whack of errors before it exits: LaTeX Warning: Reference `26529' on page xxix undefined on input line 33613. LaTeX Warning: Reference `26550' on page xxix undefined on input line 33643. LaTeX Warning: Reference `26613' on page xxix undefined on input line 33673. LaTeX Warning: Reference `26821' on page xxix undefined on input line 33703. ! TeX capacity exceeded, sorry [save size=5000]. \setnormal ...ing \let \fPosture \pageFontPosture \let \fProportionateWidth ... l.33711 {start}} \Link% ! ==> Fatal error occurred, the output PDF file is not finished! Transcript written on postgres.log. gmake: *** [postgres.pdf] Error 1 gmake: *** Deleting file `postgres.pdf' ---- Marc G. Fournier Hub.Org Networking Services (http://www.hub.org) Email: scrappy(at)hub(dot)org Yahoo!: yscrappy ICQ: 7615664
Next: From: Bruce Momjian Date: 2003-12-22 22:26:07 Subject: Re: PDF 7.4? Previous: From: Joshua D. Drake Date: 2003-12-22 22:17:48 Subject: Re: PDF 7.4?