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The US dollar was given a boost following comments from the Federal Reserve that pressures on inflation were no longer considered transitory, as simmering uncertainty around the Greek debt crisis weighed on markets.
The greenback's move was underpinned by a more hawkish interpretation of the US Federal Open Market Committee statement, with the US dollar index reaching 75.17.
"[Fed chairman Ben]
But senior currency strategist at Royal Bank of Canada, Sue Trinh, cautioned that the statement also highlighted that the Fed would act as needed to foster maximum employment and price stability.
"In that sense, even if core inflation were to rise and the labour market was showing no signs of improvement, which seems to be the case at the moment, it doesn't necessarily mean the Fed will bring forward its tightening cycle," said Ms Trinh, who is forecasting a increase in the second quarter of 2012.
"The US dollar's rally has not been consistent with US yields, so the risk is heading into the next 24 hours, one market is going to correct and it's potentially going to be the US dollar because FX [foreign exchange] is driven by rates and not the other way around."
With uncertainty around the European debt crisis and the possible contagion effects, the euro remained under pressure, trading at about $US1.4290, encouraging funds towards "safe havens".
Mr Gibbs said given a large component of the US dollar index is made up of the euro, a weaker euro will drive the index higher.
"You've seen the US dollar improve against a variety of currencies and part of it's to do with market concern about European contagion to its own economy and potentially the global economy, which is providing some support for the US dollar."
With the Reserve Bank of Australia is no hurry to act on its tightening bias, the $A has been subject to global growth concerns, particularly Europe. Ms Trinh said uncertainty has weighed on the $A's ability to sustain rallies, and noted the interest rate market had also moved to price out any RBA tightening this year.
"The market is very complacent about the extent with which the RBA will be tightening, with inflation pressures reasonably high going forward. From that perspective, buy dips in the $A and we expect it to move back towards $US1.10 in the next couple of months as rate hikes get priced backed in," Ms Trinh said.
A speech by RBA assistant governor
The $A traded at about $US1.05 during the Sydney session.
Meanwhile, the British pound fell to $US1.6019 – its lowest since April 1 – one of the worst performing currencies as the Bank of England's minutes diminished chances of an early rate rise, and left the option for further easing.
"The pound will remain relatively soft," Ms Trinh said.
"The indications are from the minutes that they're prepared to use QE [quantitative easing] again should their economy continue to slow down as it has in recent months. Our economists pushed back rate rise expectations further out into next year."
The Australian Financial Review


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So far, we've only seen grainy pictures of Eiza and Liam enjoying each other's company. But on Thursday, the lovely Latino was all glamorous for a press conference to present the DVD of the animated movie The Croods at Papalote Museum in Mexico City.
Eiza plays Eep Crood in the Spanish-language version of the film, and has also landed a role in Adrian Cervantes' comedy All Hail the Squash Bottom Queen, reports the Daily Mail.
And here's five things we've learned about Eiza:
1. She's got ridiculously amazing, stupidly fabulous (no, really, RIDIC) gorgeous legs.
2. She admitted to having a nose job in 2011.
3. She likes a tattoo or two. Well, actually, five. She has a tree symbol on her right foot, a cross on her right wrist, a flower on her left foot, and a group of stars on her neck.
4. Her mum's famous, too: Glenda Reyna is a former model and hosted Mexico's Next Top Model from 2009 to 2012.
5. She's been single for a month after ending her two-year relationship with Mexican businessman Pepe Diaz in August 2013.
Oh, and one more thing. She has awesome ombre hair.


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How to Determine If Your PPC Campaigns Are Losing Money
image source Do you know if your PPC campaigns are actually profitable for your company? Do you want to know? Well this post is a tool you can use to ask the right questions to determine just that! Whether you are utilizing a company outside of your own to manage your PPC campaigns or you do it in house, you … Read More
Customer Buying Process Breakdown Worksheet
This worksheet is meant to help you breakdown your customer's buying process so you can see how to help them make the right decision for them and for you. It will help you to see what your customer goes through before making a buying decision. You can use this sheet for all of your customer groups. The best way to use it … Read More
How to Get More Customers from your Bus Bench Advertising
How to double your leads from your website by writing better content
When you are faced with writing content for your website, one of the most difficult things in the world is to stare that that blank page with that disrespectful cursor sitting there just blinking at you, taunting you to throw a punch at your computer monitor. Especially when it comes to writing content about your products and services, it seems … Read More
How to Get More Leads from Your Roofing Website
[vc_row][vc_column width="1/1″][text_output]In this video I critique the Avalanche Roofing & Exteriors website. They are a roofing and exteriors company in Colorado Springs that is one of the leaders in their market. They provide top quality services and a great experience to their clients which is why I chose their site to critique. In this video I will show you how … Read More


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Singer/songwriter BOBBY HEBB who's hit "Sunny" spent 15 weeks on the Billboard charts in 1966, gone at 72.
The Nashville native's tune was covered by Frank Sinatra, Ella Fitzgerald, James Brown and hundreds others.
Hebb worked the Grand Old Oprey with Roy Acuff's band and played San Francisco's Candlestick Park with The Beatles.
Born to blind musicians, Hebb spent his childhood singing and playing the spoons on the mean streets of Nashville as member of his dad's washboard band.
Hebb said that the story behind his writing Sunny was rooted in tragedy.
On November 23, 1963 the day after JFK was assassinated Bobby's bother was knifed to death outside a club.
Looking for solace in a bottle of Jack Daniel's whiskey, the tune suddenly took shape in his head.
45 minutes later he was sober and sitting on a potential fortune.
"The dark days are gone, and the bright days are here…" Hebb sang then . . . and now forever.


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Diversity In DC: How Superhero TV Shows & Comics Are Fighting The Good Fight
When it comes to LGBT representation, DC is not known for its diversity. In many ways, , but it took until Jessica Jones in 2015 for the MCU to finally represent queer characters. On the flip side, the DC TV shows have casually established a diverse cast of characters since 2012, from the bisexual Sara Lance to lesbian detective Maggie Sawyer joining Supergirl in Season 2.
This is thanks in a large part to the showrunners. Greg Berlanti recently spoke to The Hollywood Reporter about why he's dedicated to introducing as much diversity as possible to the DCTV multiverse.
So why is this is so important? Obviously, we create stories for escapist purposes, but when it comes to superhero tales these characters take on a special symbolism.
Avatars Of Identity
Superheroes are in many ways the modern idols of fiction, fulfilling the same role in our culture as the knights of legend and champions of myth. As viewers, we tend to live vicariously through the characters we see as similar to ourselves — superheroes aren't just powerful, they're empowering. Which is why it's so important to ensure that everyone has a hero they can empathize with.
Berlanti — showrunner for The Flash and executive producer for Arrow, Supergirl, and Legends of Tomorrow — explained his stance on representation to THR.
"As a kid, representation really mattered. For most of my childhood, even through college, there was a lot of feeling very alone. I loved TV, so when those very special episodes of anything came or when certain characters reflected the world I lived in, I felt connected. I wanted to contemporize these comics that I loved growing up and have them reflect the society that we live in now. It's still about working in some of those very real qualities so that everyone feels represented."
Everyone wants to see themselves become a hero, and it's good to know that the people running the DC shows understand why that's important.
The best example of a character like this in the DCTV multiverse is Sara Lance, known first as the heroic Black Canary in Arrow, then the White Canary in Legends of Tomorrow after her resurrection. Although her main love interest throughout her run on Arrow was the eponymous Oliver Queen, the Season 2 episode "Heir To The Demon" established that Sara was in fact bisexual, having had a romantic relationship with Nyssa al Ghul.
This makes Sara the only long running main character in the DCTV multiverse who happens to be queer. Legends of Tomorrow explored her bisexuality further, giving her a female love interest in the episode "Night of the Hawk", and reuniting her with Nyssa, if briefly, in "River of Time".
Sara isn't by any means the only queer character in the DC shows — police captain David Singh of The Flash is gay and married, Arrow Season 4 regular Curtis Holt is also gay, and Supergirl will introduce lesbian Maggie Sawyer in Season 2. As well as being an awesome detective, Maggie is also Batwoman's longterm girlfriend in the comics, which is an exciting prospect for Supergirl, though seeing Batwoman in the show is admittedly unlikely.
However, as fantastic as it is to see these small successes, there's still a long way to go.
Moving Forward
DC has a chequered history with LGBT characters, especially when it comes to Maggie Sawyer. Soon after Marvel had their first gay wedding (on the cover of a comic no less), the writing team on the New 52 Batwoman comic quit because DC higher ups objected to Maggie and Kate Kane tying the knot. In fact, the marriage between these two women was .
That was back in 2013. DC seem to have reversed their stance on this recently though — an issue of Batgirl last year , and one of those women was transgender. Of course, a one-off issue featuring minor characters getting married is a little different from one of DC's biggest heroes getting hitched to her longterm girlfriend, but it's still progress. Here's hoping someday Maggie and Kate can get married too. (Hey, I'd settle for Maggie making a casual reference to her wife Kate in Supergirl Season 2.)
Otherwise the DC comics have had some fantastic examples of positive LGBT representation, like . The recent continuity reset Rebirth also revealed that .
Overall, DC seems to be moving towards a more inclusive future, and the CW shows are a big part of that. There's already far more queer characters in the DC shows than in Marvel's, and with Berlanti continuing to make LGBT representation one of his priorities, we're hopeful for the future.
Who's your favorite LGBT character from DC?
[Source: , ]


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When I became a mom, I had no idea about the amount of judgments that people make about moms every day. It does not matter whether the judgment comes from another mom who does things differently than you do or from your parents, or even from random people at the grocery store – it does not feel very good. Even worse, people make judgments about things over which you have no real control, like when your child becomes potty trained.
Since every child is different, every child potty trains in his or her own time. This is such a simple concept, yet many people still cannot grasp it, or they can understand it and they choose not to. It does not matter whether your mother had you potty trained by age two, or whether the neighbor's three year old is completely potty trained while your three and a half year old still uses diapers. Like many other developmental tasks, there is a large time frame during which most children learn how to use the potty and use it regularly.
When I talk to my mom friends about potty training, we all laugh about it. Each of us uses different methods, and have had varying degrees of success. We laugh because our kids are just so different when it comes to all things potty. For example, my son will poop in the potty no problem, but he won't use the potty for pee. One of my friends has a son who does exactly the opposite. I know people who offer rewards and sticker charts, and people (like me) who don't use any method at all.
After we are done laughing, though, we commiserate about the judgments that have been tossed our way in regards to our childrens' potty training or lack thereof. There are grandparents who think that it is absurd that three and a half year old children are not fully potty trained. There are also other moms who have made competitive comments about how their kids potty trained at such and such an age. If you are a parent and you have been on the receiving end of judgmental comments regarding your toddler's progress on the potty, take heart. Each child learns to use the potty when it is right for him. Your child is wonderful, as are you. Potty learning will happen on your child's schedule, not on the schedule of those who would judge him (and you).
Photo by ZeroSilence3 on Morguefile.com.


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BioMed Research International
Volume 2013 (2013), Article ID 205183, 12 pages
Effects of Nanosilver Exposure on Cholinesterase Activities, CD41, and CDF/LIF-Like Expression in ZebraFish (Danio rerio) Larvae
1Kokshetau State University Named after Sh. Ualikhanov, Abai Street 76, Kokshetau 020000, Kazakhstan
2DISTAV, Università di Genova, Viale Benedetto XV, 16132 Genova, Italy
3Nanodiagnostics Srl, Via Enrico Fermi 1/L, 41057 San Vito di Spilamberto, Italy
4Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy
Received 22 April 2013; Revised 7 July 2013; Accepted 8 July 2013
Academic Editor: Zongli Xu
Copyright © 2013 Marzhan Myrzakhanova et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Metal nanosolicoparticles are suspected to cause diseases in a number of organisms, including man. In this paper, we report the effects of nanosilver (Ag, 1–20 nm particles) on the early development of the zebrafish, a well-established vertebrate model. Embryos at the midgastrula stage were exposed to concentrations ranging from 100 to 0.001 mg/L to verify the effects on different endpoints: lethality, morphology, expression of cholinergic molecules, and development of the immune system. (1) Relative risk of mortality was exponential in the range between 0.001 and 10 mg/L. Exposure to 100 mg/L caused 100% death of embryos before reaching the tail-bud stage. (2) Developmental anomalies were present in the 72 h larvae obtained from embryos exposed to nanosilver: whole body length, decreased eye dimension, and slow response to solicitation by gentle touch with a needle tip, with a significant threshold at 0.1 mg/L. (3) Dose-dependent inhibition of acetylcholinesterase activity was significant among the exposures, except between 1 mg/L and 10 mg/L. (4) The distribution of CD41+ cells and of CDF/LIF-like immunoreactivity was altered according to the Ag concentration. The possible effect of nanosilver in impairing immune system differentiation through the inhibition of molecules related to the cholinergic system is discussed.
1. Introduction
One of the important problems of theoretical and practical medicine and physiology is the study of the responses of the organisms to environmental cues, with the goal of enhancing prevention of the main diseases induced by environmental stress.
An emerging risk is represented by the wide diffusion of nanoparticles, such as the silver nanoparticles, which were among the first metal nanoparticles to reach the market. Manufacturers have exploited their exceptionally efficient antibacterial, antiviral, and antifungal activity [1, 2]. As reported in the reviews [3, 4], nowadays the products containing nanosilver are increasing, as well as their worldwide diffusion for industrial processes and treatments. In daily life, consumers may have nanosilver containing room sprays, laundry detergents, toys, clothing, washing machines coatings, water purificants, and wall paint. Their high catalytic activity is due to the particularly small size (from 1 to 20 nm), that highly increases the metal surfaces [1]. Nevertheless, exposure to silver nanoparticles has been associated with "inflammatory, oxidative, genotoxic, and cytotoxic consequences" [5]. According to these authors, the particles primarily accumulate in the liver and have also been shown [6] to be toxic in other organs including the brain. Thus, the balance between the advantages and risks of nanosilver employment as a water disinfecting agent is puzzling, in part due to the scarcity of validated alternative models for testing its behavior in the aquatic environment and the effects of chronic exposures.
The first thoroughly experimented studies regard the health effects of airborne nanoparticles [8], while very few is known about the behaviour of nanoparticles in the aquatic medium, so that no databases are presently available about their presence in the aquatic environment.
In this work, we have used the first developmental stages of the cyprinid zebrafish, Brachydanio rerio (Danio rerio), as a vertebrate model to test the effects of nanosilver in the aquatic environment. In this work, we will use the abbreviation ZF for zebrafish. During the last decades, this fish has been chosen as a reliable vertebrate model for understanding basic events in developmental biology.
This model possesses a high degree of homology to the human genome [9], is easy to breed in amateur aquaria, and has transparent eggs allowing the observation of embryonic development. In 48 h after fertilization, the larva is ready to hatch and development time may be easily controlled, as fertilization occurs at the first daylight.
In this model, the effects of exposures to nanosilver have been studied by several authors: silver gills toxicity was demonstrated to be effect of particulates as opposed to release of dissolved ions [9, 10]. Bar-Ilan et al. [9] demonstrated that various sized gold and silver particles were able to affect zebrafish development, causing lethality and developmental anomalies in body axis shape and heart functionality.
In aquatic organisms, pollutants are taken up by entering food, gills, and skin and rapidly are diffused to blood and distributed to all the body regions, as demonstrated in sea urchin model [11]: ingested nanoparticles were rapidly distributed to the coelomic fluids, where impaired the immune cells morphology and activity of AChE and pseudoChE.
For authors working on human health and mammalian models, the relationships between potentiation against impairment in immune system exerted by nanosilver are still controversial [12, 13]. We tried to bring a contribution by analysing in control and nanosilver exposed ZF embryos and larvae, the distribution pattern of immunoreactivity (IR) against the cholinergic differentiation factor/leukemia inhibiting factor (CDF/LIF), a cytokine molecule responsible for cholinergic differentiation and cholinesterase expression [14], and CD41+ thymocyte recruitment [14–16] as well. Actually, the interdependence of the lymphatic system with neural regulation was previously demonstrated in high vertebrates [17–21].
In this light, we also identified CD41 immunoreactive (IR) cells, which have been reported to be responsible to generate RAG2+ T lymphocyte precursors [22] as well as the expression of cholinesterases, which are well-known markers of cholinergic differentiation.
2. Materials and Methods
Adult specimens of Danio rerio were purchased from a commercial source and reared in aquaria with 15 L ultrafiltered (mesh = 0.22 μm) freshwater. The animals were regularly fed and maintained at temperature ranging between 24 and 28°C, with natural light/dark cycle.
The eggs, spawned and fertilized at the first daylight, were laid in trays covered with glass spheres at the bottom of aquaria. The glass spheres were used to prevent predation of eggs and embryos by adult specimens. Fertilized eggs and embryos were collected and allowed to further develop for 12 h at = 28°C.
2.1. Characterization of Ag NPs
Ag NPs were obtained from Polytech (Germany, type WM 1000-c), supplied as a 1000 ppm in deionized water suspension of metallic silver (Ag particles encapsulated in liposomes) with an NP size between 1 and 10 nm. This "nanosuspension" did not need further sonication, since it was very stable and it did not form any agglomerates [23]. Size range and zeta potential of Ag NPs were evaluated by dynamic light scattering (DLS) analysis (Malvern, UK).
2.2. Exposure
At 12 h dead and anomalous embryos were discarded, and the healthy ones were divided into multiwell dishes containing nanosilver particles (Ag) suspended in ultrafiltered freshwater at concentrations ranging from 100 to 0.001 mg/L.
Control eggs were maintained in ultrafiltered fresh water for the time of the experiment.
All the dishes were placed in a thermostat at = 25°C and the control and exposed specimens were allowed to develop for further 24 and 48 h, up to hatching.
After those times, the survived larvae were counted and measured. Developmental anomalies were registered and classified.
2.3. Fixation for Immunochemistry Reactions
After incubation, the living larvae were fixed in paraformaldehyde (PFA) 3% in phosphate buffer saline (PBS) + 70% cold methanol for 20 min and then rinsed and rehydrated in 0.1% BSA/PBS for 10 min before processing for immunohistochemical reactions.
2.4. Localization of Molecules Immunologically Related to CDF/LIF and to CD41
Samples were incubated overnight at = 5°C in the primary antibodies diluted 1 : 200 in PBS containing 0.5% BSA, 0.1% NGS. The primary antibodies were anti-leukemia inhibitory factor (CDF/LIF), raised in goat (Sigma, IT), or anti-CD41 (ABCAM, IT, l1024) raised in mouse. After rinsing in PBS/BSA, the samples were incubated overnight at 5°C in the secondary antibodies (chick anti-goat Alexafluor 488 and rabbit anti-mouse igG, resp.), 1: 300 in PBS/BSA. Nuclei were counterstained with 1 μM RNAase followed by 2 μM propidium iodide (PI). The preparations were mounted on a slide with the antifading gelvatol [24]. Images (1024 × 1024 × 8 bit) were acquired on a Leica TCS SP5 AOBS confocal laser scanning microscope (Leica Microsystems Mannheim, Germany), using a one airy disk unit pinhole diameter and an HCX PL APO 20x/0.70 objective; magnifications were obtained by scan field selection. Alexa Fluor 488 was excited with the 488 nm line of the Ar laser, and its fluorescence was collected in a spectral window of 500–530 nm. Propidium iodide was excited with the 543 line of the He-Ne laser, and its fluorescence was collected in a spectral window of 600–640 nm. Laser scanning transmitted light images were obtained using the 488 nm laser line.
2.5. Quantitative Analysis of ChE Activity
The control and exposed larvae (a dry volume of 100 μL for each vial) were frozen in 100 μL of 0.1 M phosphate buffer, pH7 for 24 h. Then, they were brought to the room temperature and homogenate in an ice bath by use of a minipotter homogenisator (Braun-Melsungen). The protein content of each sample was determined by the Bradford method. Of these, a quantity corresponding to 30 mg protein was used for the measurement of ChE. The lecture of the speed of substrates cleavage was performed in 0.1 phosphate buffer, pH 8, in the presence of dithiobis nitrobenzoic acid (DTNB) and bicarbonate, according to the quantitative method suggested by Ellman et al. [7], and by use of a spectrophotometer JENWAY, 6405 UV/vis, at λ = 412 nm. The absorbance kinetics were recorded for 3 min, for at least 15 homogenate fractions/each exposure. The amount of cholinesterase units (ChE U) was related to the protein content of each sample (30 mg) previously measured by the Bradford method. (one ChE U hydrolyzes 1 μmole of substrate per min at pH 8.0 at 37°C).
Acetylthiocholine iodide (ATCh), acetyl(β-methyl)thiocholine iodide (AMTCh), 1,5-bis(4-allyldimethylammoniumphenyl)-pentan-3-one dibromide (BW284C51), Tetraisopropyl pyro phosphoramide (iso-OMPA), propionylthiocholine iodide (PTCh), S-butyrylthiocholine iodide (BTCh), 5,5-dithiobis(2-nitrobenzoic acid) (DTNB), and choline chloride were purchased from Sigma.
2.6. Histochemical Localization of ChE Activity [25]
The larvae were fixed in paraformaldehyde 4% in ultrafiltered tapwater and incubated in the mixture suggested by Karnovsky and Roots [25], containing 0.03 M copper sulphate,, 0.005 M K ferricyanide, and 0.1 M natrium citrate in 0.1 M maleate buffer. AMTCh was used as a substrate for AChE reaction. The incubation was carried out at room temperature, and the development of the reaction was monitored on a light microscope. The brown-magenta staining was due to the precipitation of metallic salts at the enzymic sites.
2.7. Specificity Controls
CDF/LIF-like and CD41-like immunoreactivity (IR) controls were performed by omitting the primary antibody. Aspecific reactions were blocked by use of BSA, NGS, as above described.
The specificity of ChE enzyme activity was checked by AcTCh, and AcβMeTCh and BuSCh were selected as diagnostic substrates selective for ChE, AChE, and BChE, respectively [26]. A further specificity diagnosis was carried out by exposing homogenates before and during incubation in the Ellman et al. [7] medium to specific inhibitors, 10−4 M BW284c51 (specific inhibitor of AChE), 10−4 M eserine (inhibitor of AChE and pseudocholinesterases), and 10−4 M iso-OMPA (tetra isopropyl pyrophosphoramide, specific inhibitor of BChE). All the inhibitors were obtained by Sigma (IT).
2.8. Statistical Analysis
2.8.1. Lethality
Although the statistic elaboration of mortality data (ANOVA) did not show significant dose dependence, nevertheless a strong trend was seen for the effects exerted by the exposure to the different Ag concentrations. Thus, we calculated the relative risk (RR), showing the probability of death occurring in the exposed group versus te control cohort of larvae. RR shows the probability of death in the exposed groups versus nonexposed groups [27]. With this method, the nonexposed group (control) is always 1, because it is divided for itself, whichever is the occurrence (from 0 to 100%) of the event inside it. For example, if the occurrence of the death is 35% in the control cohort, and 45% in one exposed cohort the risk ratio for the controls will be 35/35 = 1, while it will be 45/35, that is, 1.28 times major risk of death in exposed samples.
2.9. Homogeneous Measures
For homogeneous measures (body length, enzyme activity), a one-way ANOVA was performed to test for differences among the effects of different nanosilver concentrations. Prior to running analyses, homogeneity of variances was tested by Levene's test; whenever necessary, data were transformed and retested. When transformation did not produce homogeneous variances, we set , in order to make up for the increased likelihood of type 1 error [28]. Post hoc multiple comparisons after ANOVA were made by Tukey's test of honestly significant differences. Descriptive statistics are reported as mean ± standard deviation. All analyses were performed using the free PAST software package version 2.17c [29].
3. Results
3.1. Effects of Exposures on Survival of Embryos and Larvae
The occurrence of dead embryos for each exposure was different among the embryos exposed to the different Ag concentrations, with a trend to increase from control to the more concentrated exposures, but no homogeneity among the different groups of embryos was present. The RR elaboration of the data showed an exponential risk probability of about 1.2-folds with respect to the control for the exposure to 0.001 mg/L, average risk of 1.7-folds for the embryos exposed to 0.01 mg/L, 0.1 mg/L, and 1 mg/L, and a risk of 2.56-folds for the exposures to 10 mg/L Ag concentration (Figure 1). The exposure to 100 mg/L caused 100% death in almost all the experiments.
3.2. Morphological and Behavioral Effects of the 48 h Exposures
Exposed samples presented some developmental anomalies, including scarce reactivity to stimulation exerted by the gentle touch of a needle tip. If after the third touch they did not move or their swim was inefficient, we marked a no-reply in the excel column used for registration. This feature was not dose dependent: control = 17%: 0.001 = 44%; 0.01 and 0.1 mg/L = 0%; 1 mg/L = 75% and 10 mg/L = 100%. The anomalies were classified as anomalous shape of the vertebrate column, anomalies in the otoliths shape and size (not shown). The most measurable effects were represented by the larval length (Figure 2) and by the diameter of the eyes (Figure 3). These showed a significant () threshold of effect at 0.1 mg/L, as shown in Figures 2 and 3.
3.3. Cholinesterase Activity Quantitative Assay [7]
Both acetylcholinesterase and propionylcholinesterase activities were decreased by exposures; PChE activity was always lower than that AChE (Figure 4). The AChE activity (measured by the cleavage speed of AcTChI) was always significantly inhibited by the exposure to Ag, at any concentration. In addition, a growing enzyme inhibition was present among the different exposures (), in a dose-dependent way, except between the exposures to 1 and 10 mg/L Ag.
Pseudocholinesterase (PChE) activity, measured by cleavage of PTChI, showed nondose-dependent decrease caused by exposure to crescent Ag concentrations, as 0.001 mg/L and controls did not differ in a significant way, while the exposure to 0.01 and 0.1 mg/L caused significantly different impairment (), and no significant difference was present among 0.1, 1, and 10 mg/L exposures (Figure 4).
3.4. Specificity Control of the Enzyme Activities (Figure 5)
Acetylcholinesterase activity measured by cleavage of AcTChI was impaired by BW284c51 (activity reduced by 90% in controls), while 10−4 M iso-OMPA affected the speed by a negligible percentage (activity reduced by 9.6% in controls). The inhibition percentages exerted by BW284 c51 decreased with Ag concentration, suggesting an increased amount of BChE in parallel with the decrease of AChE activity (Figure 6).
3.5. Localization of Molecules Immunologically Related to CDF/LIF (Figure 7)
In control samples, the CDF/LIF-like fluorescent immune reaction marked a complex net of vessels in the head, in the thymus (not shown), and the main trunk vessels.
For simplicity, we showed only the trunk and tail regions, containing the supraintestinal (SILV), the dorsal longitudinal (DLLV), the paracordal (PCLV), and the intersegmental lymphatic vessel (ISLV) (according to the anatomical description by Meeker and Trede [30]). In control larvae, CDF/LIF IR defined the particular structure of the vessels and of the varicosities, and the thin intersegmental vessels (ISLV), joining the DLLV. CDF/LIF-like IR also stained the walls of the vessels and molecules released among the muscle fibres (Figure 7(a)). The samples exposed to 0.001 mg/L nanosilver showed an aspect very similar to the controls; IR-positive cells were present inside the PCLV varicosities and ISLVs departing from them (Figure 7(b)). The samples exposed to higher concentrations of Ag showed decreasing distribution of positive sites (Figures 7(c), 7(d), and 7(e)). The ISLVs were not decored in the samples exposed to 0.01 and 0.1 mg/L (Figures 7(c) and 7(d)); in the PCLV the LIF IR appeared irregularly distributed and weaker in the samples exposed to 1 mg Ag/L (Figure 7(e)). Traces of released LIF-like molecules were seen inside the ISLVs in samples exposed to 1 mg/L, while the samples exposed to 10 mg/L only showed IR traces, scattered inside the DLLV, the SILV, and the PCLV (Figure 7(f)).
3.6. In Toto Staining of AChE Activity (Karnovski and Roots [25])
Figures 8 and 9 show the localization of AChE activity, revealed by use of AcMTCh as a substrate in a ZF control larvae.
3.7. Localization of Molecules Immunologically Related to CD 41 (Figure 10)
Cells showing IR to CD41 were present in the lymphatic vessels of controls and of the larvae obtained from embryos exposed to 0.001 and 0.01 mg/L (Figures 10(a), 10(b), and 10(f)), including the PCLV and the ISLV. The PCLV presented along its length varicosities (V), full of cells. The positive IR progressively disappeared from the cells contained in the ISLV starting from the exposure to 0.1 mg/L (Figures 10(c) and 10(d)) and remained localized in cell groups scattered along the PCLV and the SILV in the larvae exposed to 1 and 10 mg Ag/L (Figures 10(e) and 10(f)).
4. Discussion
Our results show a toxic effect and a death risk exerted by nanosilver exposure, inhibition of AChE and PseudoChE activity, and impaired recruitment of T-lymphocytes (CD41+ cells). The lethal effects showed a random occurrence, so that lethal toxicity was only demonstrated for the Ag suspension of 100 mg/L. Nevertheless, the risk of death showed a trend growing exponentially with the increasing concentration of the Ag suspension. As it concerns the morphophysiological and enzymatic responses to exposure, the controls and the samples exposed to 0.001 mg Ag/L generally presented similar effects, while the exposures to 0.01 and 0.1 mg/L caused statistically significant () responses, as compared to controls, followed by the effects of the exposures to 1 mg/L and 10 mg/L. This suggests the presence of thresholds for the Ag effects, probably corresponding to stages of increased catalytic activity. At these concentrations, the total area of the particles surface would reach the ability to interfere in the normal chemical reactions of the organisms, such as for instance the activity of key enzymes. These thresholds were rather congruent, although slightly different among the different endpoints. The effects found in exposed larvae were mainly due to the particulate nanosilver suspension other than to the possible ionic component. Although no news are available about the Ag(+) component, the encapsulation in liposomes prevents direct contact with water. Besides, pH of freshwater is neutral, and ionic transformation is proportional to the alkalinity of the medium, as reported by [31] and by A.G. Oromieh (http://stud.epsilon.slu.se/2358/1/geranmayeh_oromieh_a_110315.pdf).
4.1. Effects on Cholinesterase Activities
In vertebrates, acetylcholinesterase plays a key role in the modulation of neuromuscular impulse transmission, while pseudocholinesterases are mainly represented in blood plasma [32]. The role of AChE is to remove acetylcholine (ACh), the cholinergic signal molecule, from its receptors (AChRs) in the plasma membrane of the target cells. In the zebrafish early larva, the cholinergic tissues are mostly represented by the heart and the neuromuscular junctions and are scarcely present in the head and brain (see Figure 8). The tissues are characterized by different kinds of AChRs. In the neuromuscular tissue, the nicotinic AChRs are preeminent and associated to Na+ selective channels, while in the other tissues the muscarinic ones are mostly represented. These last are membrane receptors, associated to G-proteins in the intracellular domain, triggering a second messengers cascade. This cascade culminates in both nuclear and cytoplasmic events, leading to differentiative (through cGMP and protein kinases) or electrical events. In particular, the electrical events are related to the IP3 release and calcium dynamics (m1 and m3 AChRs) or to K+ outwards fluxes (m2 and m4 AChRs). In addition, AChE has been demonstrated to be a modulator of a number of nonneuromuscular events leading embryonic development [33], is a wellknown biomarker of stress [34], is involved and responsible for the control of neural and immune system interaction [35, 36], modulates cell proliferation [37] and cell migration [38], and regulates cell-to-cell communication during differentiative [39] and inflammatory events [40]. In these cases, it is able to modulate the relatively slow cell-to-cell communication related to these events, besides the high-speed signals taking place in the well established neuromuscular synapses. In some cases, such as the function in morphogenetic movements, well elucidated by Drews [38] the action of AChE may be affected by its structure of adhesion protein, which allows linking the cell glycocalyx to substrate matrices, such as fibronectin and laminin [39].
At the studied ZF stages, ChE activities in the zebrafish seem to be mainly represented by AChE (E.C., 126.96.36.199) and, at a minor extent, by pseudocholinesterases, such as PChE, able to cleave propionylthiocholine iodide. Butyrylcholinesterase (BChE, E.C. 184.108.40.206) is scarcely present at these developmental stages as shown by the scarce inhibition of AcTChI cleavage exerted by iso-OMPA in controls (Figure 5) but seems to increase its activity while AChE activity is decreasing (Figure 6, shown by the residual activity in the presence of BW284c51). This may be due to a possible role of pseudoChEs in integrating the AChE activity failure, as it has been reported by Mesulam et al. [41] in case of AChE knockout.
4.2. Possible Role of Cholinesterase in Developmental Events and Larval Health
The alteration found in the AChE and pseudoChE activities might be involved in the morphological and physiological responses of the zebrafish larvae. Actually, during the last 40 years, awareness is increased of the AChE's role in regulating cell-to-cell communications such as the ones occurring during embryonic development [38, 39], for example, inductive processes, morphogenetic cell migrations, regulation of axon growth, and neurogenesis. Moreover, ChE activities are responsible for other relevant biological events, including inflammation [40] and regulation of the balance between cell proliferation and cell death [37], as well as the modulation of cell adhesion and cell migration [38]. In particular, cholinesterase activity alteration has been shown to be impaired by NPs in sea urchin immune cells, where a reduced expression of HSP70 and GRP78 was also found [11]. This may explain the presently found effects on ZF larval morphology and on recruitment of the CD41-like IR cells. Actually, the affected larval defence seems to be decreased by the exposures.
4.3. Comparison between CDF/LIF and AChE Distribution in ZF Larvae
The distribution of AChE activity, as compared to the CDF/LIF pattern, is not completely overlapping, because in the head AChE activity is not present, except in the main sense organs (Figures 9(a) and 9(b)). In the trunk it is widely present, localized in the lymphatic vessels (Figures 8(a) and 8(b)), including the ISLV and PCLVs, where it presents the same distribution of CDF/LIF (Figures 8 and 9(f), white arrow) and in sites where it exerts its neuromuscular function, associated to the muscle, miocommas, and ventral neurons of the neural tube (Figures 9(c), 9(d), and 9(e), arrows).
4.4. Possible Mechanisms for the Found Effects
The small size of the NPs, ranging between 1 and 10 nm, may be responsible for enhanced penetration in cells and tissues, reaching the hematopoietic tissues, directly interfering in the cell-to cell communication modulated by cholinesterase activity, and impinging on differentiation of immune cells during the first developmental events of the zebrafish. The zebrafish CD41(+) cells are known to be involved in the immune system differentiation, because, after the first wave of aorta-gonad-mesonephros emopoyesis, these cells colonize the thymus to generate rag2(+) T-lymphocyte precursors [22]. These effects may be exerted independently from each other, but the CD41(+) cells recruitment may also depend on the modulatory action of the cholinergic system. In mice, T-lymphocyte differentiation was shown to be regulated by a cholinergic pathway [42].
The relationship between AChE activity and blood [43] and in thymocytes [44] is known since more than 20 years, and recently it was associated to stress events in several aquatic organisms, such as prawn exposed to ChE-inhibiting pesticides [45]. In general, the cholinergic anti-inflammatory system and α7 nicotinic receptors in macrophages have been proposed to play a role in neuroimmunomodulation of resistance and relief in mammalian inflammatory processes [46]. In the high vertebrates interrelationship between the lymphatic system cell mobilization and neurotransmitter systems was also demonstrated [16–19].
The decrease of AChE and PChE activity at doses from 01, 1, and 10 mg/L corresponding to the doses inhibiting the presence of CD41 IR cells suggests a possible function of these enzymes also in the regulation of thymocytes recruitment during early zebrafish development, or as suggested by Koelle et al. [47], pseudoChE could substitute the activity of AChE, when it is inhibited.
5. Conclusions
The effects of exposure to NPs show a trend to impairment of immune responses, possibly related to the degree of inhibition of AChE activity. This hypothesis paves the way to further studies on the presence of molecules related to the cholinergic system in the immune cells of different organisms and the competitive effects possibly exerted by the Ag NPs.
The general mechanism of action seems to be represented by a depression of the immune system, as previously stated by Falugi and Aluigi [39] in sea urchin immune cells, where not only ChE activity but also HSP70 and GRP78 expression was depressed. The general mechanism of nanoparticles on organismic health is represented by the rising of ROS and inflammation. This in turn causes or is followed by increased expression of acetylcholine [48]. This should be mitigated by AChE activity, whose task is to remove AChE from the receptors, but AChE activity is depressed by some concentrations of the Ag NPs. As a consequence, AChE inhibition is at the same time responsible for increasing inflammation by ACh and for the block of apoptosis, as it was demonstrated by apoptosis suppressions in models treated by AChE antisense [4], or by AChE inhibitors, such as organophosphorus compounds [49]. Other authors found that ACh itself, at high concentration, may exert ACh receptors blockade; this paradox was resolved by Dionne and Stevens [50] at the level of frog neuromuscular junctions.
As reported by different authors, the mechanism of action of the nanoparticles is largely unknown: in particular, it is not clear which are the NP characteristics triggering the organismic responses [51]. In general, nanoparticles do not seem to act by poisoning, but by their mechanical effect, that raise inflammation. The responses of the molecules related to the cholinergic system to inflammation have been studied thoroughly by other authors: the group of Wessler et al. (e.g., [48]), the group of Soreq (e.g. [52]), and others (e.g., [53]). On the other hand, CDF/LIF, which enhances the cholinergic differentiation, is involved in the downregulation of immune cell proliferation in case of inflammation. Thus the cholinergic system seems to be involved in the modulation of immune cells responses to inflammation, and AChE is the factor modulating the balance between inhibition and enhancement of the responses, as already stated for the effect of organophosphate pesticides on the sea urchin development [11]. However, the effects of exposure to nanoparticles seem to be more puzzling as compared to the exposures to cholinergic toxicants, such as organophosphates (OPs) and carbamates (CBs), although in a certain measure they appear similar. Actually, some induced anomalies (smaller eyes, bend trunk, etc., as described by [54]) are very similar. Nevertheless, the occurrence of the anomalies is not dose dependent as in the case of exposure to organophosphorus compounds, such as chlorpirifos or diazinon. This may be due to the different mode of action of the nanoparticles as compared to chemicals. In fact, the nanoparticles effect is due to their physical features (shape, size, degree of suspension, etc.), while the effect of chemicals such as OPs or their oxonized derivatives is due to irreversible chemical link between the phosphate group and the serine molecule in the catalytic site of the AChE molecule [51, 55–57]. Also the inhibition of the cholinergic system exerted by the Ag nanoparticles could be due to nonspecific steric competition at the level of the gorge, due to the mechanical accumulation that in some cases might act as a plug.. This could explain why the effect is rather uncertain, because the presence of the nanoparticles on the gorge in this case would be "casual" and present a threshold at certain concentrations. Otherwise, the change in cholinesterase activity might be due to a specific response to stress, as Ag nanoparticles were also found to induce oxidative stress in dose- and time-dependent manners indicated by depletion of GSH and induction of ROS, LPO, SOD, and catalase [58]. As thoroughly described by the group of Soreq, the AChE gene encoding the acetylcholine-hydrolyzing enzyme acetylcholinesterase is known to undergo long-lasting transcriptional and alternative splicing changes after stress [59]. In particular, a relationship between stress and hematopoiesis/inflammation events was established to interact with AChE molecular forms [43, 56]. These authors describe how stress can modify alternative splicing giving rise to AChE variants and suppressing micro-RNAs. The findings of these authors pave the way to understanding responses of the organisms to environmental cues, with the goal of enhancing prevention of the main diseases induced by environmental stress, including the scientific control of the possible medical application [60].
The work was supported by the INESE (IIT seeds, 2010) project. Dr. Myrzakhanova was supported by a grant e-Astana Erasmus Mundus EU programs "Kazakhstan in Italy."
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text:
HP OpenVMS Systemsask the wizard
The Question is:
Sorry for such a dumb question. How can I "loop" the show queue/full/all/by_job_status=executing command so I don't have to keep hitting reenter? thank you Mr. Wizzard time for this turtle to go home sherri
The Answer is :
Though the simplest answer is: $loop: $ show queue/full/all/by_job_status=executing $ wait 00:00:05 $ goto loop The OpenVMS Wizard would recommend learning to use DCL, and -- particularly for this case -- the f$getqui lexical function. A DCL command procedure can be created to perform most any task you want in this particular area. The OpenVMS User's Guide manual and the Digital Press book Guide to Writing Real Programs in DCL will likely be of interest here. Existing discussions of the f$getqui lexical include the following topics: (813), (1240), (2159), (3951), (4546), (4568), (4903), (5188) (5471), (5567), (5651), (5793), (5982), (6315), (6877), etc.


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2011/06/14 12:59:26
New Pizza Hut Marketing Initiative: Bait-and-Switch
I'm visiting with a friend. Last night about 6 PM he got a jones for Pizza Hut. And he was buying. He looks at this preprint that either came in his mail or his newspaper somewhere between May 31 and June 3. On the preprint are six coupons, each with an expiration date of 6/30/11. There are also three "Daily Specials" listed, for Mondays, Tuesdays, and Wednesdays. The deal for Mondays reads 2 medium 3 topping pizzas for $6 each. The ad also gives a list of stores in the area. So, we drive to a nearby store, taking the ad with us. The ESL clerk tells us they don't offer that deal anymore; they disco'd it two months ago. We showed him the expiration dates on the coupons. He offers to check in the computer. Nope, deal no good anymore. So, thinking this guy is uninformed, we go to another PH equi-distant to my friend's house. We show the ad to the clerk and ask for the deal. Clerk can't find it in the computer, so he calls a lady out from the back. She tells us they changed the prices three days ago. She offers us another 2 medium pizza deal using a coupon on the ad for more money. Underneath the three panels for the daily specials is some incredibly small type that reads, in part, "Crust availability, prices, participation, delivery areas and charges, and minimum purchase requiements for delivery may vary". So, we calculate the "Daily Special" was good on one and only one day: June 6, 2011.
Since the addresses of both stores were listed in the ad and there is no expiration date for the daily specials, this can legitimately be called bait-and-switch. On the way to a local mom-and-pop pizzeria, we discussed the fact that the clerks seemed oblivious to the ad, even though it must have been plastered all over the immediate area. We couldn't believe they didn't get any phone calls or walk-ins earlier yesterday from people who wanted that offer. We wound up with two excellent Indy pizzas for slightly more than PH's regular prices.
I think we have discussed the fact that these industrial pizza chains have dug themselves a hole by couponing. To get the people addicted to couponing detoxified, they are resorting to bait-and-switch.
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La Dolce Vita also makes an ideal gathering spot with its Bubbles N Brushes paint parties, where instructors guide novice artists through the steps of creating masterpieces.


text:
This is an archived article that was published on sltrib.com in 2013, and information in the article may be outdated. It is provided only for personal research purposes and may not be reprinted.
BYU basketball players Brock Zylstra (right) and Brandon Davies appeared at the postgame news conferenced and talked about the Cougars' 74-57 win over San Diego on Saturday night at the Marriott Center.
Davies scored 17 points and Zylstra added 10 as the seniors helped BYU coach Dave Rose get his 200th win.


text:
Daniel Montano, artist on the runJanuary 29, 2008 5:00 AM
Five years ago, a 20-year-old vandal named Michael Monack was arrested when he emerged from the Armstrong Tunnels riding a bike on an early Sunday morning.
Thanks to a tip from a motorist who saw him scribbling on the tunnel's grimy walls, Mr. Monack was about to find out that when it came to "aerosol murals," everyone's a critic.
After a brief chase that ended on the 10th Street Bridge, Mr. Monack told the cops that it was a coincidence that he was sitting in the back of their cruiser the same morning fresh graffiti appeared under his nom de plume "Mook."
At a magistrate's hearing later that week, Mr. Monack looked more nervous than notorious. He was on the hook for tens of thousands of dollars in public and private property damage -- much of it painted on difficult-to-reach surfaces like overpasses and bridge pylons.
Mr. Monack didn't generate much sympathy from property owners in the days leading up to his hearing.
Homeowners had dealt with the ubiquity of his "Mook" tags on their walls and garage doors for years and considered $100,000 straight bond more than reasonable, given the damage he had committed. He didn't have an art gallery waiting in the wings to impart a scintilla of legitimacy to his scribbling.
Fast forward half a decade. Mr. Monack has been replaced in local urban folklore by 22-year-old Daniel Montano, a scribbler who goes by the moniker "MFONE."
Like Mr. Monack, Mr. Montano will be spending quality time in Allegheny County Jail until he comes up with $50,000 straight bond. He's facing a May 12 trial for having inflicted $713,801 in public and private property damage over the years.
Unlike Mr. Monack, Mr. Montano can lay claim to being an artist -- and a very talented one.
Mr. Montano's installation of assorted furniture and abstract art is currently featured in "Gestures: Illustrations of Catastrophe and Remote Times," an exhibit at the Mattress Factory on the North Side.
When he's not inflicting thousands of dollars in property damage around town, Mr. Montano has proven himself a thoughtful assembler of "found art" and personal totems.
The description of his work on the Mattress Factory Web site provides as useful a summary as any:
"Here Montano has literally transferred elements from his home into the room: his bed, spray-painted dresser, two small shelving units, and miscellaneous found objects exist alongside intensely worked collages and wheat-pasted drawings, while fragments of text on the wall evoke disturbing images of pain and violence.
"The overall effect generates a disconcerting impression of domestic space, imbued with a dark sense of foreboding. Montano's installation forges ambiguous and unanswerable questions as to whether the fury of cataclysmic events exists in the world outside, or within, in our homes and individual selves."
Mr. Montano surrendered to the cops two days before the Mattress Factory show opened, so he hasn't had a chance to take a much deserved bow.
While assembling the show, Mr. Montano slept in his bed on site -- making himself as much a part of the show as his personal effects.
Clearly, Mr. Montano is enough of a thinker and a showman to know what the intelligentsia that frequent the Mattress Factory are interested in validating as legitimate art.
Given the stakes, why is he willing to jeopardize his future in that world by defacing public and private property and risking a long prison term?
In a recent interview with PG reporter Diana Nelson Jones, Mr. Montano said: "I don't see that what I do is nearly as destructive as what society does."
Sometimes moral incoherence of the kind Mr. Montano espouses is a pose that artists take on to sound deeper than they really are. Still, it indicates that Mr. Montano is at least thinking about his place in the aesthetic scheme of things and how to justify it.
But it's always difficult to rationalize antisocial actions as righteous. If one of Mr. Montano's victims was to wander into the Mattress Factory tomorrow and dump a bucket of tar onto his bed and furniture, would that be kosher? Could a vandal justify his or her actions by echoing Mr. Montano's glib answer to the reporter and get away with it?
Of course not. Mr. Montano would be the first to demand that the vandal be arrested. He would probably compare the incident to the assault on Michelangelo's "Pieta" by a hammer-wielding madman in the late 1970s.
Still, all true artists know that they have to suffer for their art. Why should MFONE be any different?
Tony Norman can be reached at firstname.lastname@example.com or 412-263-1631.


text:
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text:
Back in 2006, Rockne S. O'Bannon tried to sell the WB on his latest TV series, called "Cult." Although the project was reportedly a casualty of the WB and UPN merger, "Cult" has found new life on the WB's successor, The CW.
According to Deadline, The CW has given an early greenlight to a pilot episode of O'Bannon's revived "Cult;" which will be executive produced by "Gossip Girl" co-creators Josh Schwartz and Stephanie Savage. "Cult" is described a mystery drama that follows a young woman working as a production assistant on a popular TV show (also called "Cult), who soon teams up with an online journalist to investigate the rapid fans of the series who may be recreating the horrific crimes in reality.
The report also mentions that "Cult" may be set up as a limited run TV series, with only 13 episodes per season over a set period of time. However, there was no indication of how many seasons "Cult" could possibly run if it gets ordered to series.
In the original iteration for the WB, "Cult" also dealt with a rapid fanbase of a hit TV series called "Cult," but it featured two brothers as the primary characters; one of whom was to be played by future "White Collar" star, Matthew Bomer. Since the first version of "Cult" was scrapped only a week after the WB and UPN merged, the project has resurfaced at The CW and ABC before eventually coming back to The CW for its current incarnation.
Among genre fans, O'Bannon is best known for creating the classic sci-fi series, "Farscape" in addition to writing the screenplay for the "Alien Nation" movie. O'Bannon has also written for "SeaQuest DSV" and "Amazing Stories" in addition to co-creating "The Triangle" miniseries on sci-fi and his recent stint on the revival of "V."
O'Bannon's current TV project is a Syfy television show called "Defiance;" which is currently steeped in secrecy. Aside from the premise revealed at E3 that "Defiance" takes place on "a world where humans and aliens live together on a planet ravaged by decades of conflict;" all that is currently known about the TV series is that it promises to have a unique interaction with the "Defiance" MMO video game that is being produced for Xbox 360 and Playstation 3 by Trion.


text:
Prince Harry's Las Vegas party guest reveals 'drunken fumble'
A guest at Prince Harry's infamous Las Vegas party last month has claimed she shared a 'drunken fumble' with the third in line to the throne.
Carrie Reichert claims she was handpicked by the royal's entourage and asked to join Harry's party at the Wynn hotel.
When she arrived at his penthouse suite with a bunch of other girls, she alleges a game of strip billiards was already underway.
Carrie, who was born in Britain but now lives in San Diego, explained: 'Harry was already undressed. It was just crazy. He looked actually delirious. There was a pool table and he was playing air guitar with pool sticks.
'He was screaming out, "Somebody get me a glove! I'm going to do a Michael Jackson impression!"'
According to Carrie, the pair instantly hit it off and Harry, 27, soon whisked the 32-year-old to his hotel bedroom for some alone time.
Carrie told The People: 'He grabbed my hand and walked back toward his bedroom.
'He said that he liked my eyes and was just going on and on about the military. He didn't make too much sense. Then he grabbed my hand and we went back to his room.
'We kissed, he was naked at the time, and pretty open. It was a drunken fumble. It wasn't romantic, just fun.
'He was a gentleman, but he was so wasted.'
Carrie says her time with the royal was cut short because 'the alcohol affected him'
'I was there for 15 to 20 minutes', she continued.
'There was not very much talking at that time, his clothes were off. I had a bikini on. A string bikini. I had a party dress over that, and that came off. And I just had a bikini.'
Slightly confused over the exact details, she added: 'I am sure somebody stumbled in as there were people going in and out of rooms all the time. We kissed again and he said, "That was great".
'He told me I was beautiful and gorgeous, which was sweet. Then we kind of nonchalantly returned to the party and kept drinking.'
Carrie went on to insist that Harry was being egged on by his mates and that the evening was just a bit of fun and nothing more.
'[Harry] wasn't the only one naked. There were girls naked. The guys were half dressed. It was not like an orgy.
'It was almost like a game. He was trying to get everybody to get naked. That was the whole point of him being naked.'


text:
John Allen Ditullio Jr. sits in court in New Port Richey, Fla. Dec. 4, 2009
Ditullio is apparently a walking billboard for the neo-Nazi movement: a large 6-inch swastika tattooed under his right ear, barbed wire inked down the right side of his face, and an extreme and very personal vulgarity scrawled on one side of his neck.
And now Judge Michael Andrews, acting on a request by Ditullio's lawyer, has ruled that the tattoos are potentially offensive and could influence a jury's opinion in the state's death penalty case against the 23-year-old, who is accused of donning a gas mask, breaking into a neighbor's home and stabbing two people, killing one of them.
The judge ruled that any tattoos Ditullio had before his arrest won't be covered, such as a small cross under his right eye, but since his March 26, 2006 arrest the self-described neo-Nazi has added tattoos to his body that are prominently displayed and not easily concealed and public defender, Bjorn Brunvand, was worried that a jury might be biased against his client on the basis of the tattoos alone.
"Whenever someone is facing the death penalty, they should get a fair trial," Brunvand said.
The trial began Tuesday with opening statements. Proceedings are expected to stretch into next week.
Prosecutors allege that Ditullio broke into his neighbor's home and stabbed two people — injuring 44-year-old Patricia Wells, the home's owner, and killing Kristofer King, a 17-year-old visitor and friend of Wells' son.
Wells lived next door to a mobile home that was commonly known as "the Nazi compound," which had large swastika flags flying on the property, authorities said. Ditullio was arrested at the mobile home after a SWAT standoff.
Authorities called the stabbings a hate crime, and Wells agreed, previously telling local media that she believed Ditullio attacked her because she had a black friend — and because her own son was gay and Ditullio may have mistaken King for her son.
Brunvand said his client is innocent and plans to tell the jury that someone else inside the neo-Nazi compound could have committed the crime.


text: TambourineMan, - The 50 button allows you to notify other users that there is a speed trap where you are located - The "Eco" is changing from green to orange to red according the way you drive it tells you if you have an economic driving style or not. You can have an histogram of these values by tapping the speed figure. The histogram is Prius like ;-) - To switch from Km/h to Miles/h go to menu/preferences/ and change "distance unit" from kilometres to miles and restart the application. If you have any other question feel free to ask them on this forum


text:
Actually, this is an interim report more than a summary. I
asked about dialin/out modem lines with their getty blocked on
rsockt, which doesn't make sense.
First, a workaround furnished by email@example.com (Robert Andersson):
simply add "to#60" to the gettytab entry used by the modems.
The offending getty will timeout after 60 seconds, exit, and
init will produce a fresh getty without the problem. While this
does not address the underlying problem, it does prevent the
dialin lines from becoming permanently disabled and keeps one's
shop in working order. If you have Telebit T2500 modems (and
perhaps others) on dialin/out modem lines on a Sun, you should
do this.
Here is what is happening. getty thinks that it has the modem
line open. It proceeds to dup it a couple of times, so that
file descriptors 0, 1 & 2 will be open on the modem. It goes on
to condition the line with several ioctl's. It doesn't bother
to check the return values of the dup's, but it does check on
the ioctl's. The hitch is that the line is *not* open. The
dup's fail without notice. When the ioctl's fail (because they
are handed an invalid file descriptor), getty reports the
failures via syslog. syslog opens a socket to log the errors.
This open returns 0, the first available fd! The ioctl failures
are logged, but getty proceeds anyway. Satisfied that all is in
order, getty does a read on fd 0 to get a login name. But fd 0
is the socket that syslog opened, not the modem. Of course,
there is never any input on this file descriptor, so getty waits
forever. What remains to be explained is how getty gets itself
into this embarassing position.
I will continue trying to get to the bottom of this. Meanwhile,
if anybody has anything to contribute, please speak up!
This archive was generated by hypermail 2.1.2 : Fri Sep 28 2001 - 23:06:35 CDT


text:
This property has been removed by the agent. It may be sold or temporarily removed from the market
2 bedroom terraced house for saleYarmouth
Sold STC £245,000
Property Description
Full description
The properties are designed and finished to the highest standards, comprising six individually planned houses (only three now remaining), all with the benefit of high quality fittings including German Nolte kitchens, Neff appliances, oak interior doors, underfloor ground floor heating and beautifully carpeted throughout.
All houses have a spacious garage linked to a useful separate lockable storage room with power and light providing a good space for sails, bikes, and surfboards. All have a large south or west facing private decked terrace, neatly enclosed with navy blue canvas panels.
They will be sold with the benefit of a new 10 Year NHBC warranty, and are fully double glazed and partly under floor heated and have pitched ceiling on the first and second floors allowing a light and spacious feel. The garage block is located at the side of Yar Cottages with an approached by an attractive resin gravel driveway.
The property is well located being only a stones throw from the Yar Estuary with footpaths leading to miles of downland walks and the popular walk following the old railway line to Freshwater. The centre of Yarmouth is within a few minutes walk, where a good range of facilities can be found including restaurants, pubs, sailing/yacht clubs and shops and the regular ferry service to Lymington.
LOUNGE/DINING/KITCHEN 12' 1" x 26' 8" (3.68m x 8.13m) Substantial solid oak stable front door gives access to this large open plan room via the kitchen area. The kitchen is well equipped with contemporary stainless steel German Nolte units with Neff appliances, together with a stainless steel sink with drainer to side. There is a 4 hob cooker, with separate integrated oven and extractor hood over. Door to lobby. Window facing east, and French doors leading to private patio/terrrace.
LOBBY A bright and airy two storey lobby with floor to ceiling height windows fill this area with light. Doors to cloakroom and lounge and stairs to first floor.
CLOAKROOM Hand basin, WC, and extractor fan, and space for washing machine.
BEDROOM 1 12' 1" x 10' 7" (3.68m x 3.23m) A good sized bright and airy double bedroom, walk-in cupboard. West facing aspect and an Estuary glimpse.
BEDROOM 2 12' 1" x 9' 1" (3.68m x 2.77m) An east facing double bedroom with skylights and loft access above.
BATHROOM A high quality suite comprising of large stand in bath with separate shower unit over, wash hand basin and WC. Towel rail, and extractor fan, ceramic tiles throughout.
OUTSIDE A resin gravelled driveway at the side leads to garage en bloc and to the enclosed lockable storage room. To the front of the property is an enclosed west facing patio/terrace accessed via steps from Mill Road.
VIEWINGS All viewings will be strictly by prior arrangement with the sole selling agent.
MISDESCRIPTIONS ACT 1991 Spence Willard for themselves and the vendor of this property whose agents they are give notice that:
i The particulars are set out as a general outline only for the guidance of intending purchasers and do not constitute, nor constitute part of, an offer or contract.
ii All descriptions, references to condition, necessary permissions for use and occupation and other details are given in good faith and are believed to be correct, but any intending purchaser should not rely on them as statements or representations of fact and must satisfy themselves by inspection or otherwise as to the correctness and accuracy of each of them.
iii No person in the employment of Spence Willard has any authority to make or give any representation or warranty whatever in relation to this property.
More information from this agent
To view this media, please visit the on-line version of this page at www.rightmove.co.uk/new-homes-for-sale/property-33764962.html
Listing History
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Market Info
Disclaimer - Property reference 100523001563. The information displayed about this property comprises a property advertisement. Rightmove.co.uk makes no warranty as to the accuracy or completeness of the advertisement or any linked or associated information, and Rightmove has no control over the content. This property advertisement does not constitute property particulars. The information is provided and maintained by Spence Willard, Yarmouth. Please contact the selling agent or developer directly to obtain any information which may be available under the terms of The Energy Performance of Buildings (Certificates and Inspections) (England and Wales) Regulations 2007 or the Home Report if in relation to a residential property in Scotland.
Map data ©OpenStreetMap contributors.


text:
Scandal First Look: Who Is Olivia Marrying in the 100th Episode?
Apr 6, 2017 10:01 PM EDT
Would Olitz be living happily ever after if Olivia hadn't rigged the election?That's certainly what the promo for Scandal's 100th episode implies. The hour will take place in an alternate reality, exploring what life would be like if Olivia (Kerry Wa… Read more


text:
As a former soap star-turned-international pop sensation, Kylie Minogue might be one of the coolest aunts around, but she admitted she's can't get the idea of babies out of her head.
The Australian pop star told Glamour UK that she looks "wistfully" at sister Dannii Minogue's baby boy, Ethan, and also revealed that she has looked into ways she could too could join the motherhood fray.
"I've looked into various options," Kylie told the fashion magazine, according to the UK's Press Association, which obtained excerpts from the interview.
Among the options the "Love At First Sight" singer has "thought about," include egg donation, Kylie reportedly told the mag.
"I don't know if I'm going to go down any of those roads yet, but I do need to look at what might be potential paths that lead to a family," she reportedly added. "They can do incredible things now, especially in America."
Kylie also opened up about her recovery from breast cancer, noting that she is getting her body back into top form.
"I don't want to lose any more weight. I just need to get my fitness up. I do feel that then I'll almost be back in my own body, which would be great," she said. "I felt for such a long time that I was in a stranger's body."


text:
It's rumoured that Giuseppe Sacco once said: > [please CC in reply, since I am not subscribed to the list] > > Hi, > I jst read the announcement about pgAdminIII and I am very happy. > I tried to install it in my debian box, but I found that the binary > version is available only for i386. I then decided to recompile > everything from source and send it back to you, but I have now this > problem: Hi Guiseppe, I'm not sure about the Debian side of things, but the wx source code is in the wxWindows directory on the ftp servers (it was only put there this morning so may not be on all of them yet). There are compilation notes in docs/en_US/unix_compilation.html in the pgAdmin source tree that should help. If you need help with the Debian packaging, then Raphaël Enrici <blacknoz(at)club-internet(dot)fr> is our Debian packager. Regards, Dave.
Next: From: Mark A. Taff Date: 2003-08-09 06:07:21 Subject: Re: pgAdmin III Bugs/Feature Requests Previous: From: Dave Page Date: 2003-08-08 19:01:39 Subject: Re: pgAdmin III Bugs/Feature Requests


text:
Online dating with New Scientist Connect – "find chemistry with other singles here" – has just been launched to bring like-minded people together, according to today's New Scientist newsletter.
"Whether you're looking for love, or just to meet someone on the same wavelength, no matter where you are in the world," the message continues, with a link to "let your love life lift off," with a free one month subscription.
New Scientist is part of RBI, parent company of ICIS.
photo: New Scientist


text:
The Palm Beach Post January 28, 2001 Sunday
WANT TO STAY SAFE? KEEP OUT OF SPACEEliot Kleinberg, Palm Beach Post Staff Writer
The seven who died aboard the Challenger on this day 15 years ago weren't the first American astronauts to perish in the line of duty, but their shuttle's explosion on live television shook a nation complacent in its space supremacy.
It was wake-up call to the dangers of space travel - a tragedy some experts say is as relevant today as ever.
NASA says it has employed 312 active astronauts and trainees during the half-century of the space program.
The 17 deaths calculate to 5,449 deaths per 100,000 astronauts - far above the 162.5 deaths per 100,000 fishermen, the most dangerous profession listed by the U.S. Department of Labor.
Though the figure's statistical value could be debated because of the small sampling, no one disputes that the vacuum of space and the miles of thin air between it and Earth constitute just about the most dangerous workplace possible.
"With all this ballyhoo of space tourism and moving into space, it's like it's taking a trip to West Palm from New York," said State University of New York professor Karl Grossman, author of The Wrong Stuff and producer of two video documentaries strongly critical of NASA's safety record.
Grossman also is concerned about the dangers to civilians from accidents involving spacecraft carrying plutonium or nuclear reactors.
"I'm worried about people all over the world being impacted by this public Pollyanna attitude by NASA toward the dangers of space travel," Grossman said.
Even in orbit, astronauts face a man-made minefield. NASA counts more than 150,000 orbiting objects of at least 1 centimeter in size; 2,720 are operating or abandoned spacecraft or parts of them, ranging in size from 110 pounds to Russia's 154-ton Mir space station, set to fall to earth in March.
It may be no coincidence that the American astronauts and Soviet cosmonauts died not in the deadly reaches of space but either sitting on the pad or during launch or reentry, said John Pike, director of GlobalSecurity.org, a nonprofit, nonpartisan public policy organization supported by private foundations.
GlobalSecurity deals with defense and space issues. Pike has argued that many space missions could be handled by unmanned spacecraft without endangering people.
"It's not so much that being in space is dangerous as coming and going is," Pike said from Virginia.
But, he added, "Everything we've done thus far, if you got into trouble you had at least the possibility of bailing out in somewhere between a few minutes and a few days.
"The shuttle is never more than a half hour from landing. There's normally very little margin for error in spaceflight generally, and dramatically less margin of error in deep-spaceflight."
Pike notes that recently two Mars probes in a row vanished; had they been manned, he said, "You'd have a lot of dead people."
Also ran South.
Copyright 2001 Palm Beach Newspapers, Inc.


text: We're heavy AFS users here at Boston University, and it's going to be a major blow to not have an AFS client available. OpenAFS, last I looked, said that it'd take them a year to properly develop a 2.6 version *if* they got some funding to do so -- and they don't. Arla doesn't seem to be going anywhere. So that leaves the in-kernel AFS -- which was apparently developed by someone at Red Hat. (An enterprise customer needed it, perhaps?) I haven't played with this much, but I tried and failed to get it going in Fedora Core 2 test 1. I'll need to speak with our AFS gurus to really figure it out. But in the meantime, Can someone point me to further information, or nicely tell me about, Red Hat / Fedora's plans in this regard? And what I can do to help? (From a tester's perspective at the very least.) Thanks! -- Matthew Miller mattdm mattdm org <http://www.mattdm.org/> Boston University Linux ------> <http://linux.bu.edu/>


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NATHANIEL COOPER, son of Ezra Cooper, married Phebe Barton, eldest daughter of Benjamin Barton, Esq., and removed to Leon, Cattaraugus County, N. Y., where he occupied a prominent position, for a long time, doing the larger share of public business. His son JOHN has many of the characteristics of his father, has been Representative, Supervisor, and held other offices.
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Iraqi President Fouad Massoum nominated a new candidate to replace Prime Minister Nuri al-Malaki, but the move has sparked a stand-off, as Maliki says he plans to remain in power.
In northern Iraq, Islamic militants still roam the country where they've been attacking several cities in an attempt to consolidate power. In response, the White House has launched airstrikes to support the Iraqi and Kurdish forces, but the US has stopped short of putting troops on the ground.
Douglas Ollivant, senior National Security Fellow at the New America Foundation, talked to Take Two on Monday about the situations brewing in Iraq.


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November 10, 2011, 09:02 AM
anyone have guidance on using a cable lock for a shotgun? since this is my first firearm i dont want to damage it and i havent seen a good explanation anywhere. I know it's supposed to run through the chamber but it's not clear to me what the best way is. My gun is Beretta AL391 URIKA2. Thanks.


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Julia Roberts Bikini Pictures in Hawaii
Julia Roberts Flaunts Her Bikini Body on a Family Vacation
Julia Roberts wore a bikini over the weekend for a swim! She was off on a family vacation with her husband Danny Moder and their three children, Hazel, Phinnaeus, and Henry in Hawaii. The 44-year-old Mirror Mirror star treated herself to the family getaway during her children's Spring break. It also happened to coincide with the conclusion of her press duties for her Snow White picture, which hit theaters on March 30. Over the holiday, the movie came in fifth at the box office with just over $11 million.
There's more film work to come in Julia's future as well. She's signed on to star in A Normal Heart, another project with her Eat Pray Love director Ryan Murphy. Also, Julia will team up with Meryl Streep in the big-screen version of the hit play August: Osage County.