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Truth Sets In tab by Avi Buffalo, added: May 5th, 2011
Profile History
+ --------------------------------------------------------------------- + | Ultimate Guitar Tabs Archive - your #1 source for tabs! | | http://www.ultimate-guitar.com/ | | | | Over 1,000,000 guitar, guitar pro and bass tabs! Also lessons | | news and guitar forums! | + --------------------------------------------------------------------- +
This song needs desperately to be tabbed better, so here's my take. I am very confident that this is at least 95% right. There may be a few transition chords that are played briefly that I'm missing. But on the whole this is what Avi is playing. (And the second guitarist.) * = harmonic h = hammer on / = slide BRIEF INTRO: G G G D, G G G D HARMONICS: |-------------------------------------7*-------| |-12*-------------------12*--------------7*----| |----12*--------5*---------12*-----5*----------| |-------12*--7*---------------12*--------------| |----------------------------------------------| |----------------------------------------------| Then, that is interrupted by the following: KEY CHANGE: A G (D) A G |------------------------------------------| |------------------------------------------| |------------------------------------------| |--6-----7-6-7-6-4------9-6-----9-6-4-6-4--| |------------------------------------------| |------------------------------------------| A G (D) A C D C |--------------------------------------------------------| |--------------------------------------------------------| |--------------------------------------------------------| |--6-----7-6-7-6-4------9-6-----6h7/9----14-14-14-14--12-| |--------------------------------------------------------| |--------------------------------------------------------| CHORDS FOR VERSES: VERSE 1: G what's another time to say Bm witches speak in my head all day (G) Bm witchcraft seems to unload and say Am you don't love me anymore G what's another word to scream Bm maybe one more about the team (G) Bm try to improve the local scene Am we ain't no good anymore Then, HARMONICS section again, without segue to KEY CHANGE section. VERSE 2: (same as verse 1: G Bm Bm Am) wait till the truth sets in pay no attention to how we've been you know that i thought too much of when i made my fatal mistake hope for the consequence picture your people still on the fence pretend that you hope that it's ok when it's no good anymore Then, HARMONICS section, twice as long as first time. Finally, KEY CHANGE section once more. And that's the song.
the key change should be on the 3rd string
verse chord pattern should be G-Am-Bm-Am (with a quick A flat minor in between the Bm and Am) other than that this is excellent!


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This was my first project ever! Since I taught myself how to knit from a book, I did some screwy things for awhile like always knitting into the back of my stitches which is why this scarf is full of twisted stitches. I still wear this all the time.
12383 projects
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subset: commoncrawl


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Libraries and Other Research Links
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Bunnell Family History Center
400 N Palmetto St.
Tuesday 10-2, Wednesday 10-2, Thursday 10-6, Saturday 10-2
From Daytona, take I-95 N. Get off at exit #91. Go west on Moody Blvd (Route# 100) for 3 to 4 miles. Palmetto St. is on your right at the edge of Bunnell after the road curves to the left. It is just after the municipal waterworks. Alternately, go north on Route#1 to the traffic light at the center of Bunnell, turn right a few blocks and the FHC will be on the left.
Genealogy Society of Flagler County
P. O. Box 35-4671
Meeting Location
Flagler County Library
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"Songas is a tribute to the commitment of people who had the vision of gas-fired generation in Tanzania and took ownership to achieve that vision. There were so many reasons for this never to happen, but people believed enough and made the vision a reality."
—Paul Kunert, Chairman of the Board, Songas
Provide cost-effective energy and reliable performance in a remote location with a challenging grid.
Aeroderivative gas turbines with fast ramp rates, water efficiency, and load-following capabilities.
Tanzania has long depended on the region's water resources to produce hydroelectric power, but ongoing drought conditions have forced the country to rely on other power generation resources, specifically Songas' ability to generate 180 MW of electricity using natural gas resources from Songo Songo Island's gas fields.
In 2004, the Songas gas processing, pipeline and Ubungo power station were commissioned. Songas and GE installed four LM6000 Aeroderivative Gas Turbines to help power the country. Despite the distance from major suppliers and engineering facilities, Songas has achieved one of the highest availability rates in all of Africa — historically always above 95 percent and 96.6 percent in 2012.
The added benefit of these particular units is that they do not require water for operation, making them an environmentally friendly alternative energy source. The severe drought experienced in 2006 reduced hydro-based generation by 88 percent. In 2011, another drought reduced hydroelectric generation by 66 percent. Coupled with an existing energy gap of 33 percent, even if all hydro sources were operational, Tanzania faces severe capacity shortages as demand continues to increase. With this diversion away from hydroelectric power, the importance of Songas' natural gas-driven turbines in Tanzania has become much more critical. In fact, Songas now provides nearly a quarter of all of Tanzania's electricity.


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Báo QĐND English
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The Vietnamese Garment and Textile Association, based on the Thang Long Garment and Textile Businesses Club, made its debut in Moscow on April 21.
Do Quy Duong was elected the Chairman of the Association, which aims to promote the exchange of information and strengthen cooperation among Vietnamese businesses in Russia.
The Association will also to provide its members with legal consultations and cooperate with the Vietnam Pharmaceutical and Medical Association (VPMA) in Russia to provide better care for workers.
Vietnamese Ambassador to Russia Pham Xuan Son congratulated the establishment of the organization, which he said will help strengthen unity among the overseas Vietnamese community and encourage them to contribute more to the homeland.
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GMC Designer on Denali XT Hybrid Concept
GM Energy Director on E85 and Flex-Fuel
GM Exec on the GMC Sierra Two-Mode Hybrid
Coskata CEO on E85 Partnership with GM
GM VP on Energy Diversity - Video
GM Energy Director Discusses Fuel Cells
GM VP on Green Technology and Volt - Video
GM Interior Design Renaissance - Video
GM VP Nesbitt on Future of Design - Video
2008-2012 GMC Yukon Hybrid Review
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GMC - Brand in Transition Auto Show Video
General Motors Exec Bob Lutz - Interview
GM Exec on Two-Mode Hybrid Pickups


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MILPITAS, Calif., Feb. 10, 2014 (GLOBE NEWSWIRE) -- FireEye, Inc. (Nasdaq:FEYE), the leader in stopping today's advanced cyber attacks, today announced FireEye ® MVX-IPS, a new approach to Intrusion Prevention Systems (IPS) that modernizes the IPS security layer by incorporating FireEye MVX technology and FireEye Dynamic Threat Intelligence™. FireEye MVX-IPS is designed to provide a more comprehensive view of multi-vector attacks to boost threat protection against known and unknown attacks. FireEye MVX-IPS is now available in Beta and will be generally available as an add-on license for the FireEye Network Threat Prevention Platform (NX series) during the first half of 2014. Current IPS products are deficient for two reasons -- they are signature-based and therefore unable to detect modern threats, and they create excessive alerts, requiring customers to devote additional resources to manage their system. FireEye MVX-IPS takes a new approach to IPS by extending FireEye's Multi-Vector Virtual Execution™ (MVX) technology to a new layer in the security stack, eliminating both problems created by traditional IPS solutions. The unique architecture of FireEye MVX-IPS allows validation of attacks identified by IPS, thereby reducing alerts and allowing security teams to focus on genuine threats. This approach consolidates threat prevention for known and unknown threats, increases the efficacy of defenses against known threats leveraging multi-protocol signatures, and optimizes time and resource investments needed to identify, validate and resolve these threats. "While intrusion detection technology has held the promise of advanced threat detection for some time, it has generally failed to deliver by remaining too reliant on a signature-based approach. This has been one factor in moving the market towards alternative detection products such as STAP (Specialized Threat Analysis and Prevention)," said John Grady, research manager, security products at IDC. "The problem is that in most cases, the consolidated IPS functionality remains heavily signature oriented, thus only marginally improving security. Integrating STAP functionality directly with dedicated IPS technology, creates a more efficient and effective approach to detecting advanced, targeted attacks and greatly improves an organization's security posture."
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text: I had to reset My mid7024 and when I tried to reinstall Angry Bird RIO and Seaons they will not work. My 4 year old loves them and I would like to put them on for him. Also would be nice to have space too but it will not run either. Sugestions?


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Dinner Dance at the Hotel Bethlehem
On February 19, the Hotel Bethlehem will host a dinner dance in their Grand Ballroom, featuring jazz music by members of the Moravian community. The cost of $25 per person (plus tax & gratuity) includes a three-course dinner (chef's Hotel Bethlehem salad; duet of pecan chicken and glazed salmon; accompaniments; dessert and coffee); cash bar.
The event is open to the public but the hotel will seat Moravian alumni and members of the College community together. For reservations, call Hotel Bethlehem directly at 610 625-5000; be sure to tell them you are with Moravian. Come support Moravian musicians and enjoy a special evening.
Mark Your Calendar
Nominations Open for the 2011 Hall of Fame Induction
Three Moravian alumni recently were elected Presidents of the Bar Association in their respective counties: Jamie Swartz '86 (Lehigh County, PA), Kerry Freidl '85 (Northampton County, PA), and Brian Fruehling '84 (Morris County, NJ). Swartz and Freidl both attended the University of Bridgeport School of Law (now known as Quinnipiac University School of Law). Swartz is a partner to Donald Spry '69 at the firm King, Spry, Herman, Freund, and Faul; Jim Ravelle, Moravian professor of sociology, law, and public management, is "of counsel" to the firm. Freidl practices family law primarily at Hemstreet, Nitchke, & Freidl in Easton, Pa. Fruehling, a 1987 graduate of the University of Richmond School of Law, is a litigation attorney based in Madison, NJ.
For more Alumni news, visit: www.moravian.edu/alumni.
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I had closed rhinoplasty 6 months ago to remove a dorsal hump (see photo) by rasping top and sides. I'm very unhappy with the results so far as my dorsal hump is still showing. What kind of changes should I still expect? Am I stuck with this hump or will it absorb? Also does the area above the tip still look swollen? I would appreciate any advice, as I'm very depressed about my surgery results. My surgeons knows my concerns but he's just protecting his work. I'd like another opinion please.
I Had Closed Rhinoplasty 6 Months Ago- Unhappy With Results. What Can I Expect From Final Result? (photo)
Doctor Answers 19
Unsatisfactory results after closed rhinoplasty--what to do.
At 6 months post-op, most (but not all) of your swelling has resolved, scar tissue softened, and sensation and circulation recovered. Based on your photos, you have:
- Inadequately-reduced nasal bones, at least with relation to the slightly-more reduced upper lateral cartilages, causing the visible protrusion at the osseocartilagenous junction, and the lack of a smooth nasal profile. This may require osteotomies if an "open-roof" deformity results from additional dorsal bone reduction
- Supratip fullness, possibly secondary to scar tissue (the most common cause), but also possible due to inadequate correction of the alar cartilage configuration. This also contributes to:
- Sagging nasal tip (inadequate upward rotation of the alar cartilages) and lack of supratip "break"
- (No front view available), but if the tip remains too bulbous: inadequate treatment (resection, scoring, and refinement) of the alar cartilages (part of #2)
This is not a result of closed vs. open rhinoplasty, as experts in each method could have achieved better results. Rather, this simply requires more (better) surgery. The timing of surgery is best delayed until there are no further changes being noted, which takes 6-12 months.
If the relationship with your rhinoplasty surgeon is good (sounds like it may not be if your assessment is that he is "protecting his work"), then by all means discuss your exact concerns and expectations with him, including costs. If you are unsure of this, you may wish to seek second opinion elsewhere from ABPS-certified plastic surgeons experienced in rhinoplasty revision or Board-certified otolaryngologists who specialize in revision rhinoplasty. This deserves to be better. There will be additional costs, though your previous surgeon would likely not charge any additional surgeon's fee.
BTW, although HA fillers can improve the appearance temporarily, it does "muddy the waters" for subsequent assessment or surgery, so do not do this unless there is a long delay prior to repeat surgery. Best wishes!
Learn more: http://www.mpsmn.com/photogallery/nose-surgery-rhinoplasty-5912
Unhappy with rhinoplasty result 6 months after surgery
At 6 months, your results from a closed rhinoplasty are pretty stable. Based on your photos, revision certainly seems necessary to improve the result and address your concerns.
In a secondary case I would do it in an open fashion with a general take down of the dorsum, particularly to deal with the step off at the proximal third of the dorsum. The tip needs to be elevated slightly to improve the angel between your lip and columella and also you need a better transition to the tip to remove the poly-beak appearance.
This could be done at any time and should result in significant improvement. Good luck and thanks for sharing.
Learn more: http://www.delucaplasticsurgery.com/rhinoplasty-albany-ny/
I Had Closed Rhinoplasty 6 Months Ago- Unhappy With Results. What Can I Expect From Final Result?
You may be nearing the final results at 6 months post Rhinoplasty but you may want to ask your Rhinoplasty Surgeon these questions. Since no front view was included, it's difficult to see if residual tip swelling exists or if there is an open roof deformity (dorsal hump rasped down without osteotomies to break the nasal bones inward). From the photos, there appears to be some residual dorsal bone above and dorsal cartilage below a centrally placed indentation. The nasal tip also appears to be under-rotated. Hope this helps.
Learn more: http://www.drfpalmer.com
You might also like...
6 Month Result after Closed Rhinoplasty
You do have significant improvement compared to your pre-op appearance, but I do understand your concerns. Although you will wit another 3-6 months I do feel you will have a slight residual hump and a small polly beak which will necessitate a revision. Minimal refinement will give you an excellent result.
Rhinoplasty revision.
You should discuss the result with your surgeon since I don't know what was said before surgery. Your result won't change much and I'm guessing you will need a revision. See an experienced revision rhinoplasty surgeon for a second opinion before proceeding.
Unhappy with rhinoplasty results
I believe that your result may be improved. I would recommend you wait at least 1 year after the original surgery before doing a revision. This will allow for the majority of edema to resolve. Donald R. Nunn MD Atlanta Plastic Surgeon.
I Had Closed Rhinoplasty 6 Months Ago- Unhappy With Results. What Can I Expect From Final Result? (photo)
Agree you will need a revision and 6 months is the minimum time to wait but I recommend at least 2 to 4 more months.
Rhinoplasty revision
When I assess patients for dorsal hump removal before surgery I measure the amount in millimeters to remove and how much of the hump is cartilage vs. bone. Sometimes it's 50:50 other times it more cartilage or bone. The reason is because I need to know how much to remove at surgery and the bone responds better to rasping or filing than the cartilage. I cut the required amount of cartilage off and then extend this upward to get the required amount of bone out. Your before photo shows the hump. The after photos definitely show less of a hump but the contour is not smooth. This could be addressed with some additional rasping and the bottom outer edge of the septal cartilage could be taken down a bit more. I would not consider these major revisions of your surgery. It is also far better to take out insufficient amounts than to take out too much as you can always take out more. If you take out too much though replacing the bone/cartilage can be problematic. As long as you have not lost faith in or communication with your surgeon the best person to do that revision is your current surgeon. You just need to decide on timing and that is best done when the swelling has mostly resolved. 6 to 9 months is usually sufficient especially since the revision is not major.
I hope you realize that this format of posting questions and receiving answers lacks the face to face direct communication required for you to make an informed decision regarding your surgery.
My response to your question/post does not represent formal medical advice or constitute a doctor patient relationship. You need to consult with i.e. personally see a board certified plastic surgeon in order to receive a formal evaluation and develop a doctor patient relationship.
Rhinoplasty, Nose syrgery
By this time, 6 months after rhinoplasty, your swelling has subsided and more drastic improvement should not be expected. Even though your dorsal hump is moderately reduced, there is room to have more reduction, and to achieve more balanced profile. This is not a very difficult procedure and more pleasing result could be achieved with relatively short period of recovery time.
Irregular Nasal Dorsum after Closed Rhinoplasty
Dear Rhinoplasty Patient,
I reviewed your nasal profile pictures and it appears that there is insufficient dorsal reduction. The cartilaginous portion of nasal septum at supratip area is still high and there remains some excess bony nasal bone on top. This has created a dorsal irregularity and dip in the middle of your nose. Your may want to wait another six months and have a revision rhinoplasty. I recommend an open rhinoplasty to correct this problem. Just in case you're hesitant to have another surgery, a non-surgical rhinoplasty using fillers would be a good option as well. Good luck, Dr. Kevin Sadati
Learn more: http://www.orangecountynosejob.com/
These answers are for educational purposes and should not be relied upon as a substitute for medical advice you may receive from your physician. If you have a medical emergency, please call 911. These answers do not constitute or initiate a patient/doctor relationship.


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I am not in the habit of quoting Newt Gingrich approvingly. The former House speaker hasn't changed much since he was the bulldog and bullhorn of the Republican Party in the 1990s, throwing every wrench he could into Bill Clinton's presidency. Even today his primary prescription for our national salvation is the same one-note refrain it has always been: reduce taxes, particularly on wealth.
More voodoo economics, that's all they've got.
But in a recent interview with the St. Petersburg Times editorial board, Gingrich did say something about the tea party that rang true. "The tea party people are the natural heirs of the (Ross) Perot movement," Gingrich said, and just as with Perot's followers, there will be a "natural tension" between the tea baggers and the Republican Party. For Republicans to obtain a governing majority, Gingrich continued, they will have to find a place for the tea party at a "family picnic that's pretty strange," full of folks with highly disparate views and interests.
Gingrich is posing a challenge and a warning to the Republican Party. About 75 percent of those who identify themselves as tea baggers say they are or lean Republican. The movement followers' undifferentiated anger and motivation could be a source of activism for Republicans come November.
But to co-opt the movement the Republican Party will have to figure out what it stands for, and that is going to be tricky. The tea party hasn't coalesced enough to clarify a platform beyond a fierce distaste for President Barack Obama, health reform and government debt.
When more specifics on the group's leanings start to gel, they quickly devolve into political quicksand. Last week, a couple of their leaders on CNN's Larry King Live, Dana Loesch and Wayne Allyn Root, said they would do away with Social Security if they could.
Planks like this and other similarly radical ideas such as the one floated by Wisconsin's Rep. Paul Ryan, the ranking Republican on the House Budget Committee, who would balance the federal budget by turning Medicare into a capped voucher program, would be a big problem for the tea party and other Republicans.
The groups would lose their seniors in an Ozark minute.
From everything I've read, the tea baggers appear to be an inscrutable mass of angry people, each with their own gripe. They want a small government, a balanced budget and tax cuts just so long as nothing touches their Medicare, Social Security, veterans' benefits, federal disability payments or unemployment checks.
Then there are people like Jeff McQueen, who was featured in a recent New York Times article about all the unemployed people in the tea party ranks. McQueen organizes tea party groups in Michigan and Ohio. He's angry because the government allows free trade, which he believes led to the loss of his job selling auto parts.
Why is a man who wants government to more tightly control the economy in a movement that seems to call for the opposite? This is the kind of all-over-the-map incoherence that will frustrate Republican attempts to corral the group's supporters.
Another foreboding aspect for Republicans of Gingrich's parallel between the tea party and Perot relates to electoral impact. Gingrich says Sarah Palin is the new Perot in that she fills the same "emotional and ideological zone." (Just change out Perot's academic graphs and charts with Palin's hand checklist.) If she ran for president in 2012 as an independent after dropping out of a Republican primary, she could clinch Obama's re-election, just as Perot's 1992 run handed Clinton the presidency.
A new poll from Quinnipiac University bears this out. It found that voters say they will vote 44 percent to 39 percent for a Republican over a Democrat in a congressional contest this November. But add a tea party candidate and the vote becomes 36 percent Democrat, 25 percent Republican and 15 percent for the tea party candidate.
There isn't much on which Gingrich and I agree, but I do think that the Republican Party will have to handle this tempest in a tea party very carefully or risk getting scalded.


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Los Angeles, CA
Guller-Padilla Designs commissioned us to produce and manage a full set of 3D Design layouts and drawings for a low cost housing solution for the housing problem in Southern California. 3D Computer generated renderings were produced to convey the ideas for a new housing project that utilizes steel containers as the shell structure for residential units. Initial designs and concepts were developed, implemented and produced in AutoCAD and then rendered using VIZ software. The new project is currently in the final stages of development in Palm Springs. Land was purchased and is currently being further developed to sustain a community of these low cost steel container residential units that will revolutionize the low income housing development industry.
Status: Built
Location: Palm Springs, CA, US
My Role: BIM Modeler
Additional Credits: Contract work for Guller/Padilla Designs


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Wicked Tickets
One of Broadway's most popular shows, Wicked tells the unbelievable backstory behind The Wizard of Oz's Glinda the Good and Elphaba, the green girl that becomes the Wicked Witch of the West. This family-friendly musical is the definition of a Broadway spectacular, with award-winning sets, costumes and special effects.
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- The most complete, and completely satisfying, musical I've come across in a long time.
- Elysa Gardner, USA TODAY
- What a spectacle this is! Keep your eye on the witches. These wonderful girls from Oz are a delight.
- Linda Winer, Newsday
- Built on songs by Stephen Schwartz and a book by Winnie Holzman, the show is steeped in talent.
- Ben Brantley, The New York Times
Wicked is the untold musical story of The Wizard of Oz's Wicked Witch of the West and Glinda the Good... before Dorothy dropped in. Based on the imaginative Gregory Maguire novel, the visually-stunning musical Wicked takes a fantasy journey through the unseen side of Oz, sharing a tale of unexpected friendship and love. A mega hit for over ten years, Wicked on Broadway is full of memorable tunes including "Defying Gravity," "As Long As Your Mine," "Popular" and "The Wizard and I."


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COMMERCE CITY, Colo. (August 17, 2011) — The Colorado Rapids twice came from behind in their CONCACAF Champions League debut to grab a dramatic 3-2 victory over 10-man Isidro Metapán of El Salvador.
Macoumba Kandji, making his first start in his first game since MLS Cup 2010, emerged as the hero, scoring a brace and creating Andre Akpan's winning goal.
The game could not have started more dramatically. With barely 15 seconds on the clock, Metapán forward Cristian Bautista was pulled down by Drew Moor in the penalty area. The penalty was duly converted by Allan Kardeck to give Metapán and their numerous fans a shock lead.
Los Caleros' lead, however, was short-lived. In the 16th minute, Kandji marked his return with a header to tie things up. Left completely unmarked just yards from the goal, the lanky striker turned in Scott Pagulta's cross.
Kandji's goal lit a fire under the Salvadoran club, and barely nine minutes later, Metapán icon Paolo Suárez put his team back on top. Rapids goalie Matt Pickens, who had just been upended in the penalty box, carelessly threw the ball towards Kosuke Kimura. The Japanese right back was not ready to receive the ball, which was pinched by an alert Suárez. The Uruguayan then chipped the sphere over Pickens and into the empty net.
With a handful of minutes left in the first half, the visitors went down to 10 men when Kardeck was sent off for a a stray arm into Moor's face as the striker chased ball. The Rapids took advantage of their extra man and right before halftime, Kandji struck again, bundling the ball in from close range to level things once more.
Kandji's second tally seemed to calm some Rapids' nerves after a helter-skelter first 45 minutes. The goal, coupled with the man advantage, give Colorado a big boost of energy heading into the second stanza.
Just five minutes into the second half, Kandji and Wells Thompson combined well before Thompson dialed a ball into Akpan, who shot crisply into the net with his left foot to give the Rapids the lead for the first time in the match.
Metapán, apart from some pressure in the last few minutes, found it difficult to create many chances as the Rapids increasingly found spaces behind the Salvador team's defensive line.
Quincy Amarikwa, a second-half substitute for Sanna Nyassi, had a golden chance to stretch the Rapids' lead early on in the second period when he found himself alone on goal, but he could not control Thompsons' cross.
Thompson himself also had a great chance in the 73rd minute after a perfect through ball from Akpan, but his shot was saved by 'keeper Miguel Montes.
The win gives the Rapids three points and has them tied for the Group B lead with Mexican side Santos Laguna, who defeated Honduras' Real España 3-2.
Colorado return to Dick's Sporting Goods Park Saturday for an MLS game against Chivas USA. Their next CCL game is on Tuesday, when they travel to face Real España.


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There have been a lot of rumors floating around about the possibility of an Alice in Chains / Deftones / Mastodon tour. You probably haven't heard anything about this from us -- we don't report rumors, dammit! I actually knew all along that this tour would come to fruition (perks of being and 'industry insider') but held off reporting it until I had something concrete. Now I do because the respective bands have officially announced the said tour and have even released some dates. Tickets -- probably costing you your first born -- will go on sale May 15th here, so jot that down on your calendar and we'll try to keep you updated on dates and prices when we know more. You can see the dates and locations of the tour released thus far after the jump.
9/16 Chicago, IL @ Charter One Pavilion at Northerly Island
9/17 Detroit, MI @ DTE Energy Music Theatre
9/18 Toronto, ON @ Molson Canadian Amphitheatre
9/20 Uncasville, CT @ Mohegan Sun Arena
9/22 Boston, MA @ Agannis Arena
9/24 New York, NY @ Madison Square Garden
9/28 Atlanta, GA @ Aaron's Amphitheatre at Lakewood
10/1 St. Louis, MO @ Scottrade Center
10/4 Denver, CO @ Red Rocks Amphitheatre
10/7 Vancouver, BC @ General Motors Place
10/8 Seattle, WA @ Key Arena
10/9 Portland, OR @ Rose Quarter @ Memorial Coliseum
10/16 Las Vegas, NV @ The Joint
Chief Editor and Writer for Metal CallOut. Favorite sub genre of heavy metal includes Black, Death, Folk and Traditional. Josh can be found at Google+.


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It's about 14 years I saw this wonderful film, in the Portuguese TV. Five years later, I reviewed it in Film School with a different perspective, as I had the opportunity to feel such atmosphere in Cuba, few months before. Heddy Honigmann's approach to the passenger POV inside a taxi in Lima (Peru) is quite similar the experience you can have traveling inside a taxi in Havana. The old models, rebuilt to the last part, as a living frame to the city, as an itinerant exhibition in the gallery of colors, lights, sounds and faces of the daily beat. Barely, we get out of the cars. The city itself, is a living entity that interacts with the viewer without asking permission. For every driver, we get a new puzzle piece - a new intimate narrative - which allows us to understand the beliefs and the expectations of those people.Overview from themoviedb.org
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This star rating is the opinion of the property owner only. A Self-Rated hotel has not been assessed by the AA or Visit Britain, whose official star ratings we use across LateRooms.com.
Sunway Putra Hotel5 Self rated
9th Floor , Sunway Putra Place, 100 Jalan Putra,, Kuala Lumpur, 50350
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About the hotel
Advantageously located along Kuala Lumpur's diamond triangle, the integrated development of Sunway Putra Kuala Lumpur is anchored by the 36-storey Sunway Putra Hotel, the newly reopened Sunway Putra Mall and an office tower. Only 45 minutes away from KL International Airport (KLIA), Sunway Putra Hotel is exactly opposite the Putra World Trade Centre (PWTC) and is also directly connected to the public train lines via covered pedestrian walkways. Besides all this, the hotel also provides scheduled shuttle service to KLCC and Pavilion.
Sunway Putra Hotel Kuala Lumpur offers 650 well appointed guestrooms and suites that comes with complimentary Wi-Fi access for the convenience of our guest(s). Our modern and elegantly furnished rooms are equipped with a flat-screen TV, minibar, tea/coffee maker and ironing facilities while the en suite bathroom offers toiletries and a hairdryer. Guests can relax and rejuvenate at the hotel's fitness centre with state-of-the-art selection of fitness equipment or take a leisure dip in our 25-metres long outdoor swimming pool. Our flagship restaurant, the Coffee House serves authentic local dishes, signature Asian cuisines and sought-after Western fares or you may explore the contemporary Japanese fare at our newly opened Gen Restaurant with signature dishes such as the Sushi and Sashimi or the Gen Bento Set. Guests looking for a more relaxed setting can indulge in our delicious specialty coffees and pastries at the Atrium Lounge.
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- Hotel Non-Smoking Throughout, Air conditioning in rooms, 24 Hour Reception, Cots available, Safety Deposit Box, Tea/Coffee making facilities in each room, Hairdryer in each room, Iron And Board In Each Room, Minibar in each room, Telephone In Each Room, 24 Hour Room Service, Limited room service for hot food, Concierge, Laundry/Valet Service, Wake Up Call Available, Currency Exchange, Interconnecting rooms available, Porter, Lift to all floors, Public Air Conditioning
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Normally I'd preface this piece by saying when I see a bunch of nonsense Chinese small cap stocks rallying for weeks on end, combined with multiple parabolic moves in established "growthy" stocks it has traditionally marked a level of egregious actions by the bulls, that would indicate a short to intermediate term top. But I have been offering that logic for 3-4 weeks, and in the new paradigm market 'old school' views have been rendered moot. Hence, I will just say "buy stocks, QE2 fixes everything".
OpenTable (OPEN) reported last evening, and it appears to be the latest stock (in a now crowded line) to enjoy a "Riverbed day" - it is opening in the +15% range, and appears to be the new Netflix (NASDAQ:NFLX). So to answer my question from last month [Sep 16, 2010: Does One Dare Short OpenTable?] - the answer is "at your own peril".
Via Reuters
- OpenTable Inc (OPEN) the restaurant reservation platform, reported a higher third-quarter profit that topped Wall Street estimates as more restaurants used its services. The San Francisco-based company reported a net profit of $3.8 million, or 16 cents a share, compared with $896,000, or 4 cents a share, a year ago. Excluding items, the company earned 23 cents a share.
- Revenue rose 44 percent to $24.5 million.
- Analysts had expected a profit of 15 cents a share, excluding exceptionals, on revenue of $23.2 million, according to Thomson Reuters I/B/E/S.
- The company's installed restaurant base as of Sept. 30 was up 31 percent at 15,246, and seated diners jumped 54 percent to 15.9 million.
- The company generates substantially all of its revenue from its restaurant customers. OpenTable's revenue includes monthly subscription fees and a fee for each restaurant guest seated through online reservations.
- OpenTable has more than doubled in value this year.
Trader Marker


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Still no closure for Pasco teacher accused of manhandling student
A month ago, the Pasco School Board overruled superintendent Heather Fiorentino's plan to fire Jeffrey Bush, a teacher at the Irvin Educational Center. Bush had been accused of violently grabbing a student who made lewd comments about his wife.
Even though the board sided with Bush, the story isn't over.
Fiorentino has stated that she will reinstate Bush to another post in the district, but she does not intend to repay him the salary he lost while suspended without pay. Bush's lawyers and union argue that such a move is against the law.
Florida statute 1012.33, section 6b, says in the case of a school employee being charged with misconduct, "if the charges are not sustained, he or she shall be immediately reinstated, and his or her back salary shall be paid."
"Heather Fiorentino's intransigence on an untenable position is not reason for him to suffer," United School Employees of Pasco president Lynne Webb said.
But Fiorentino contends that, while the board didn't agree with her choice of penalty, neither did it clear Bush of wrongdoing. In fact, she said, Bush and his lawyers admitted his grabbing of a student. And that makes him ineligible for the back pay, she suggested.
"They won Round 1, and now they're going for Round 2," Fiorentino said.
Lawyers for both sides are scheduled to make statements on the issue to the School Board on Oct. 6.


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NEW YORK ( TheStreet) -- U.S. stock futures were suggesting Wall Street would open lower Monday as Japan's economy grew slower than expected in the June quarter. European stocks were trading to the downside while Asian shares ended Monday's session mostly lower. Japan's Nikkei 225 fell marginally to close at 8,885.15. Japan's economy grew at a slower-than-expected annual rate of 1.4% in April-June. This follows reports of weaker-than-expected trade data from China on Friday.
U.S. stocks on Friday finished with gains on renewed belief from investors that the Federal Reserve will prop up the economy. The Dow Jones Industrial Average climbed 41 points, or 0.3%, to 13,206. The index closed up 0.9% for the week. The S&P 500 rose 3 points to 1,406. It closed up 1.1% for the week. The Nasdaq finished up 2 points to 3,021, climbing 1.8% for the week.
Republican presidential candidate Mitt Romney chose Rep. Paul Ryan of Wisconsin as his running mate for the November election. Romney made the announcement on Saturday in Norfolk, Va.. As chairman of the House Budget Committee, Ryan has been a vocal proponent of conservative fiscal policy, advocating steep spending cuts in social programs and an overhaul of Social Security and Medicare.
Activist investor Nelson Peltz will join the board of Ingersoll-Rand ( IR) as he pushes to improve profitability at the industrial conglomerate, The Wall Street Journal reported, citing people familiar with the matter. Peltz's Trian Management LP fund took a roughly 7% stake in the company in May. The company is expected to announce Peltz's appointment to the board on Monday, the people told the newspaper.
Groupon ( GRPN) is expected by analysts Monday to post second-quarter adjusted earnings of 3 cents a share on revenue of $578 million. In the first quarter, the daily deals site posted a profit of 2 cents a share on revenue of $559.3 million. Shares of Groupon have declined more than 60% since its IPO in November was priced at $20.
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PRZOOM - /newswire/ -
Oakland, CA, United States, 2010/06/11 - Entrust Administration, Inc. has launched Entrust IRA for iPhone® and iPad, a new application for investors on the go.
Entrust Administration, Inc., is proud to present Entrust IRA for iPhone and iPad, a new application that empowers investors to take control of their financial future from the palm of their hand. This innovative and educational hand-held tool, now available for download from the App Store℠, is a convenient source of information for investors seeking to grow and diversify their retirement portfolio.
Entrust IRA for iPhone and iPad includes features that allow users to log in to their Entrust account, get the latest updates on industry news and asset-building strategies, and learn about different types of investments allowed in a self-directed retirement plan. Theres even a handy feature devoted to prohibited transactions, per IRS code. With the app, users can determine Required Minimum Distributions based on IRS guidelines, and compare Roth and traditional IRAs, with Entrusts practical calculators.
This new app has the features investors need to keep up with the pace of todays world, says Hugh Bromma, CEO of Entrust. It gives our customers complete access to their Entrust self-directed retirement plan, and offers them tools to make an informed decision, all in a convenient mobile format.
For more than 28 years, Entrust Administration, Inc. (entrustcalifornia.com) has been the premier provider of account administration services for self-directed retirement plans. Entrust is an acknowledged authority in the field of self-directed retirement accounts, and is committed to the goal of financial independence, especially during retirement. They are the leader in self-directed plans, custodial services, and educational curriculum to build wealth with investments that clients know, understand and control.
Apple, the Apple logo, and iPhone are trademarks of Apple Inc., registered in the U.S. and other countries. iPad is a trademark of Apple Inc. App Store is a service mark of Apple Inc.
Agency / Source: The Entrust Group
Availability: All Regions (Including Int'l)
Traffic Booster: [/] Quick PRZOOM - Press & Newswire Visibility Checker
Distribution / Indexing: [+]


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The Joy of FRED Blog
Cheryl Collett, Itty Bitty Foodies - Yummy Adventu, on 01/09/11
This cracks me up. I want some for my two boys!
John at PVD, on 01/10/11
Cute ninja ginger bread. This is what I hate, I don't like eating them because they are too cute to destroy :)
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Amin Kawar & GAC Jordan Support Siraj Program
Save the Children signed a Memorandum of Understanding (MoU) with Amin Kawar & Sons Co. (shipping & transport), according to which the company, in cooperation with GAC Jordan, will support the Siraj youth leadership program, which is being implemented by Save the Children in cooperation with a number of governmental and non-governmental institutions that work for the youth, and which is funded by the United States Agency for International Development.
As part of the partnership, GAC Jordan and Amin Kawar are shipping 650 Siraj Leadership Tool Kits to five Arab countries where the youth leadership program is being implemented, which includes Egypt, Jordan, Lebanon, West Bank/Gaza and Yemen. The MoU was signed by Ms. Nadia Shahin, General Manager of Amin Kawar & Sons Co. and Ms. Paulette Hassell, Co-Country Director of the Save the Children's offices in Jordan & Lebanon.
The Siraj Leadership Tool Kits were launched in Egypt during The Regional Youth Forum - a regional event that took place between August 6-8, 2010. The Tool Kit presents unconventional leadership competencies, case studies and skills to be examined and shared at national and regional levels, ensuring that emerging leaders are at the forefront of positive development approaches.
Ms. Hassell highlighted the importance of the MoU stating, "Save the Children believes in the importance of building strong partnerships with the private sector as they play an extremely important role in supporting our development projects."
Ms. Hassell highlighted the important role and support of Amin Kawar & Sons Co. and GAC Jordan which has allowed Save the Children to provide hundreds of thousands of youth across the five participating countries with Siraj Leadership Tool Kits. She added that Save the Children has been working in Jordan since 1985 and it is a global non-profit organization that brings a lasting and positive change to the lives of children, youth and families in more than 120 countries around the world.
Fadi Nasser, General Manager of GAC Jordan underlined the importance of GAC's cooperation with Save the Children to reach out to the largest number of youth possible across the region by utilizing GACs top quality services and total shipping solutions. Nasser went on to say that he values the efforts put forth to execute such a successful initiative by Amin Kawar and highlighted GACs commitment to the development and support of the local community, especially the youth.


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Spoke Talk: Green-knuckle riding
July 26, 2013
Steamboat Springs — Where is the trail? I know it's there. I can feel the smooth dirt under my tires as I roll through the turn by rote. As the turn finishes and my bike returns to an upright position, the trail reappears through a screen of green foliage. Looks like we will have to dedicate a workday to this trail before it vanishes into the Yampa Valley jungle. The foliage around here does not need much water to grow, and Emerald Mountain is a perfect example of the never-ending growth that encroaches on singletrack trails until they barely are visible.
Trimming the foliage around the trail and re-establishing the corridor is thankless work that often goes unnoticed, but it is essential to keeping the trail rideable and open.
We have many miles of trails in the valley, and most of them need regular maintenance just to keep them as they are. Much of that work falls on a few dedicated members of Routt County Riders and the volunteers who graciously show up to our trail workdays in exchange for food and beer. We hope they get something else out of it also — perhaps the knowledge that they have done their part to keep our trails established and accessible.
There are many more mountain bikers in this valley than miles of trail. If every mountain biker gave one four-hour volunteer shift per summer, we would stay ahead of the game and never lose sight of the trails behind a sea of green.
On one recent work session, we were trimming the foliage that had gotten overgrown on Larry's with a volunteer group of six. During our four-hour work session, we were passed, and usually thanked, by almost 100 riders. Of all the riders, I only recognized two from previous trail workdays. Although the riders were thanking us as volunteers, it's Routt County Riders that really deserves the credit.
The group spends much of its limited budget on these trail workdays. This summer, $5,000 has gone toward trail maintenance, and another $1,000 was spent on additional tools. The money Routt County Riders spends is much more than the organization brings in through membership dues and is just a fraction of the value given to the community when all the volunteer hours are taken into account. Routt County Riders is able to do this through grants, donations and community support, and the more members we have, the easier it is for us to raise these funds.
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As trail design evolves, our trails are becoming more sustainable and require less maintenance of the tread surface, but the vegetation never stops. It grows relentlessly, and as we add more trails, we will need more bikers and other trail users to give a few hours back to the trails that give them so much enjoyment.
Aryeh Copa is a board member of Routt County Riders.


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Florence Faure
Florence Faure studied in Paris. She got her M.S. degree in immunology from the University Paris VI and Pasteur Institute in 1985 and her Ph.D. degree from the University Paris VI in 1989. During her PhD in Villejuif, France, she was involved in the very early description of the TCR ?/d human T-lymphocyte subset. She described molecular, phenotypical, and functional differences among ?/d T lymphocytes circulating in human blood. Her research on MHC-independent target cell/T lymphocyte interactions led her to start the analysis of CD1/TCR molecular interactions both in France and in the UK (in the Laboratory of Pr Cesar Milstein, LMB, Cambridge). At the beginning of the 90s, she described for the first time the negative regulatory role of CD223 molecule in APC/CD4 lymphocytes interaction. She then started to study cancer immune response, in melanoma and breast cancer. She pointed out the bias using in vitro culture both for T cells and tumor cells analyses. For a melanoma patient, she characterized the tumor infiltrating lymphocytes and described the large repertoire of tumor specificities among CD8 T cells concomitantly with the local expansion of some given tumor-specific T-cell clone. In collaborative works, she also characterized the molecular basis of differences between primary and metastatic cell lines for T-cell recognition and cytotoxicity. Faure is now studying in Institut Curie, Paris, the characteristics of cross-presentation required for an efficient induction of tumor immune response.
Biography Updated on 4 October 2011


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The aim of the VENUS-C Open Call is
to expand the user community, gather additional requirements for the VENUS-
platform, test and validate it by funding between 10 and 20 new pilot prototypes
with applications suited to the Cloud. The Call is open to European public and
private research organisations involved in Research and Technology Development
(RTD) from the 27 Member States and the 13 Associated Countries (Annexes). The
Open Call targets a spectrum of application areas (Annexes), spanning the Arts &
Humanities, Engineering, Health & Life Sciences, Economics and Financial
Services, Natural Sciences, as well as maths and science with application
characteristics such as dynamic scaling requirements, peak demands and
ubiquitous availability. Specifically VENUS-C aims to bring on board research
groups that have operational and scientifically productive application software
already running on platforms, whether commodity clusters, HPC clusters, grids or
VENUS-C will work closely with the new pilots to determine what features and
capabilities of Cloud computing environments are needed to support the type of
computing exemplified by the selected pilots, and to validate the VENUS-C
infrastructure in this aim. To this end, the pilots will have collective access
to 6.000.000 CPU hours per year of Windows Azure and 40 TBytes of storage plus
additional CPU hours and TBytes available from other VENUS-C providers. Seed
funds will be provided to catalyse take-off. VENUS-C will also provide technical
support to address specific the specific needs of the pilots and enable users
through innovative web-based and physical training. The pilot prototypes will
have access to the VENUS-C infrastructure from July 2011 until May
2012 with a one-year extension to the Azure and other platforms until May
2013. Visibility will be ensured through focused VENUS-C dissemination
activities, including the support of media partnerships.
VENUS-C would like to thank the eScienceTalk project and the following media
partners for their support of the Open Call: International Science Grid This
Week (iSGTW, to be re-named The Digital Scientist), Supercomputing Online,
Science|Business and EnterTheGrid.
Complete Open
Call Documentation: Application Form
and all the details, including the procedures, full list of target scientific
areas, eligible countries, expectations and evaluation, and an FAQ.
Open Call Launch: Tuesday 11 January 2011.
midnight CEST Monday 11 April 2011
Evaluation notification: Monday 2 May 2011.
You may submit the completed Application Form through the on-line form below.
However, to facilitate processing, we recommend you send the word version to
Proposers will receive by return email a PDF version of the Application Form for
verification with a number, randomly allocated, for internal purposes.
Acknowledgement of receipt does not represent endorsement or acceptance of the
proposal. All Proposals will receive a confidential evaluation in May 2011.
VENUS-C is committed to selecting the best possible pilots. Please feel free to
contact us via email (email@example.com) for any assistance,
marking your message "VENUS-C Open Call Enquiry" before the 11 April 2011
Brief CV of the Proposer
(<500 words):
State-of-the-art in the field
and expected advance
Description of the
positioning of the project
among other contributions in the domain (<2000 words):
Describe briefly the main motivations behind porting the application to the cloud
(e.g. limitations of the current approach) (<500 words):
What are the potential benefits for the Proposer and its scientific community in
using VENUS-C? (e.g. reducing processing time, increased capability to run
larger problems, new features, improved management of resources, improved
exploitability, etc.) Is it a key activity in your research?(< 500 words):
Has any other similar application been migrated to cloud? (if yes, please provide
references <500 words):
Please add any other
requirements associated with the application.
Degree of maturity of the
application in its current status:
Has the application already
been adapted to a Cloud infrastructure?
Has the application already been adapted to another Distributed
Computing Infrastructure
Will the Proposer Group be
able to participate in demonstration and dissemination actions (in terms of
Describe the software dependencies needed to run the application, indicating
origin and requirements.
Indicate if the application
has dependencies on databases
Computational, storage and
bandwidth resources for the whole experiment
Will usage consist in a
single execution or a periodic repetition of executions?
Unique experimentRepeated usage, indicate number of monthly executions:


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SEEKINGRhythm Guitar, Lead Guitar.
-We are looking for a second guitarist. Check the music out, and if it sounds like something you'd be interested in get ahold of us! Thx!!
Calveris is a heavy metal band hailing from Toledo, OH. They have been steadily honing their sound through high energy shows while developing into a relentless and dynamic band. Their debut album "From Sorrow to Strength" was released in spring of 2009. With no end in sight this fun loving, beer swigging group of friends have stayed unwaveringly focused on bringing their unique brand of metal to the masses abroad.
- Screen name:
- calveris
- Member since:
- Apr 06 2010
- Active over 1 month ago
- Level of commitment:
- Very Committed
- Years together:
- 4
- Gigs played:
- 50 to 100
- Tend to practice:
- 2-3 times per week
- Age:
- 34 years
Pantera, Machine Head, BLS, Lamb of God, Down, and a sh*t ton more.
Members Of Band
Josh Haas- Guitars
Ryan Mullins- Vox
Justin Bailey- Bass
Matt Austin- Drums
Architects of Manipulation
Architects of Manipulation Track number: 1 Year: 2009 Album: From Sorrow to Strength Genre: Metal
Within Our Grasp
Within Our Grasp Track number: 10 Year: 2009 Album: From Sorrow to Strength Genre: Metal


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Felipe Massa has the cards stacked in his favour: he's starting from pole position at Valencia with the benefit of the clean side of the grid – now the organisers have swapped the grid around. But will the F1 pack get through the tricky first corners without a crash? What are their strategy options for … Continue reading Thoughts on qualifying and the start (2008 European Grand Prix)
Circuito Urbano Valencia articles archive
Circuito Urbano Valencia (Valencia street circuit)
Ferrari engines fill top four places in speed traps in qualifying at Valencia
Posted on
So much for Mercedes giving McLaren a 25 horsepower advantage – the engine doing the business at Valencia this weekend is the Ferrari. Here's who set the top four maximum speeds in the second sector of the lap at Valencia (where the highest speeds are reached) during qualifying today: Position Drivers Car Speed 1 Sebastian … Continue reading Ferrari engines fill top four places in speed traps in qualifying at Valencia
Felipe Massa pips Lewis Hamilton to pole (2008 European Grand Prix qualifying)
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Felipe Massa took pole position for the first ever European Grand Prix in Valencia. He will rejoin his battle with Lewis Hamilton from the Hungarian Grand Prix as the McLaren driver took second place on the grid. Robert Kubica will start from third place ahead of Kimi Raikkonen. But the star of the session was … Continue reading Felipe Massa pips Lewis Hamilton to pole (2008 European Grand Prix qualifying)
2008 European GP qualifying live blog
Posted on
The European Grand Prix qualifying live blog has now finished. You can review the session in full below:
2008 European GP practice 3 live blog
Posted on
Join in the European Grand Prix free practice live blog below. Here are some useful links: Links to live F1 video (please post any new video links here) Live F1 timing Radio commentary (UK) To read earlier messages in the live blog it will be easier if you turn off automatic scrolling using the button … Continue reading 2008 European GP practice 3 live blog
First impressions of Valencia's F1 track
Posted on
I've been surprised by some of the negative reactions to the new Valencia F1 track. Watching the practice sessions yesterday I found a lot to like about the circuit. The setting is a bit more industrial than we expected but the parts where the circuit passes alongside the harbous look good and there are some … Continue reading First impressions of Valencia's F1 track
First pictures of the Valencia F1 street track (2008 European Grand Prix)
Posted on
The Circuito Urbano Valencia saw F1 action for the first time today. Before the race weekend I wasn't sure how good the track would look – footage from the first race at the circuit made the track look a bit featureless. But I thought it looked better during today's free practice session. Here's a selection … Continue reading First pictures of the Valencia F1 street track (2008 European Grand Prix)
2008 European GP practice 2 live blog
Posted on
The second free practice session for the 2008 European Grand Prix has now finished. You can review the live blog in full below.
2008 European GP practice 1 live blog
Posted on
The European Grand Prix free practice 1 live blog has finished. You can review the session in full below.
2008 European GP predictions
Posted on
Yesterday I made a case for why I thought Robert Kubica would do well at the new Valencia street track. But there are plenty of convincing reasons his McLaren and Ferrari rivals could find the Valencia circuit suits them well. Here are my predictions for the top three in the European Grand Prix ?����ǣ share … Continue reading 2008 European GP predictions
Robert Kubica the best outside bet? (2008 European Grand Prix preview)
Posted on
Seeing the BMWs of Robert Kubica and Nick Heidfeld at the sharp end of a Grand Prix isn't really a surprise any more – not since their one-two finish at Montreal anyway. The team are yet to re-capture the high Kubica's maiden win. But I think Kubica could be a dark horse for victory this … Continue reading Robert Kubica the best outside bet? (2008 European Grand Prix preview)
European GP history 1984-2007 (video)
Posted on
Here's the second part of Journeyer's video guide to the European Grand Prix. Read part one here. One reason that the European Grand Prix became a regular round of the championship can be summed up in two words: Michael Schumacher. His popularity was such that from 1995 Bernie Ecclestone decided to have 2 races in … Continue reading European GP history 1984-2007 (video)
European GP history 1950-1997 (video)
Posted on
F1 Fanatic guest writer Journeyer is back with a two-part series on the history of the European Grand Prix. The European Grand Prix is an oddity. It's unique in that it doesn't necessarily represent a single country, but a whole continent. Various versions of the name have been in use ever since the world championship … Continue reading European GP history 1950-1997 (video)
Spain's other Formula 1 street circuits: Pedralbes and Montjuich Park
Posted on
The Circuito Urbano Valencia will become the sixth Spanish circuit to host a round of the world championship this weekend. It will also be the third Spanish street track to hold a round of the championship. The previous two such circuit – Pedralbes and Montjuich Park – were lost from the championship following concerns over … Continue reading Spain's other Formula 1 street circuits: Pedralbes and Montjuich Park
Toyota European GP preview (Video)
Posted on
This video includes footage of the new Circuito Urbano Valencia including the attractive pit buildings in the old port warehouses and the novel swing bridge (which presumably will be kept closed during the race…) Toyota's engineers explain how they are preparing for the track including the very high braking demands they expect to encounter.
Valencia: Hockenheimring with walls?
Posted on
It's becoming clear from what the drivers are saying about the Circuito Urbano Valencia that it's not a typical street track. Here's a look at a lap of the circuit and some of the ways in which it seems more like Montreal than Monaco and other famous street circuits.
Drivers and teams explain how they prepare for the Valencia street track
Posted on
The European Grand Prix next weekend will be held at a new circuit for the teams: the Circuito Urbano Valencia in Spain. The venue is so new it only held its first race a few weeks ago, so how are the teams preparing for this leap into the unknown? Here's what Fernando Alonso, Nico Rosberg, … Continue reading Drivers and teams explain how they prepare for the Valencia street track
Final tyre allocations good for McLaren?
Posted on
Last week Ferrari suggested the harder tyres Bridgestone have brought to some races have played into McLaren's hands. If that is what's going on the red team are likely to be disappointed by the tyre allocations for the final race of the season where Bridgestone again are bringing a harder set of tyres than last … Continue reading Final tyre allocations good for McLaren?
Ferrari want softer Bridgestone tyres
Posted on
An interesting article on Autosport yesterday gives some more insight into how McLaren have suddenly got the upper hand in the world championship battle. Ferrari believe part of McLaren's advantage has been because the tyres supplied by Bridgestone at recent events have suited the MP4/23 better than the F2008. With Bridgestone yet to announce their … Continue reading Ferrari want softer Bridgestone tyres
Video: Valencia street track TV footage
Posted on
Here are two more videos of the Circuito Urbano Valencia, which will host its first F1 race in three weeks' time. I posted some onboard video from the Spanish street track earlier this week which started us wondering how good the circuit would look on TV. This footage should give us a good impression:


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The FAA issued Information for Operators (InFO) 09005 last week, urging proper pilot training for boot operation and maintaining airspeed in de-icer-boot-equipped airplanes. Although the FAA cites only one accident in the InFO–the February 2005 Circuit City Citation 560 crash in Pueblo, Colo.–it is possible that the Colgan Air Q400 accident near Buffalo, N.Y., in February also prompted publication of this information. The InFO recommends that all training and checking personnel ensure that training and checkrides address operating limitations and procedures for flight-in-icing conditions, the ice-bridging issue and when to deploy boots, appropriate airspeeds and monitoring skills and workload management during icing operations. The FAA also suggests that training center evaluators and check airmen test de-icer boot knowledge and operation during practical tests and flight checks. Pilots should also ensure they use the manufacturer's recommended airspeed in icing conditions or a speed 1.5 times the non-ice stall speed, the FAA said.
FAA Recommends Added Icing Training for Boot Fliers
- April 29, 2009, 5:11 AM


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________________________________ From: Josef Springer [mailto:Josef(dot)Springer(at)JOOPS(dot)COM] Sent: 04 August 2005 13:35 To: Dave Page Cc: pgsql-odbc(at)postgresql(dot)org Subject: Re: [ODBC] Usind database with encoding UNICODE and vovel He Dave, as other wrote, there should be an encoding problem with Windows 2000. My Environment: Windows 2000 internal version: 5.0.2195; SP 2 Germal locale PostgreSQL: 8.0.3 ODBC driver: 8.00.01.01 (5. March 2005) OK, I'll say this again - have you tried 08.01.0003? I have little time to help, and I'm not going to chase bugs that might easily have been fixed already in newer versions of the driver than you are running. No MDAC The ODBC Driver manager is part of MDAC - you cannot use ODBC on Windows without it. If you are running the default version that comes with Windows 2000, then you should try upgrading to the latest (which is what works for me on XP) http://msdn.microsoft.com/data/mdac/default.aspx Regards, Dave.
Next: From: Josef Springer Date: 2005-08-04 13:45:45 Subject: Re: Usind database with encoding UNICODE and vovel Previous: From: Josef Springer Date: 2005-08-04 12:35:29 Subject: Re: Usind database with encoding UNICODE and vovel


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Dementia costs are rising as the baby boomers age. Long-term care costs now exceed cancer and heart disease combined!
The Statistics
The New England Journal of Medicine recently published significant data about just how much Dementia costs us. Not surprising, as we are now paying attention to the growing numbers of older adults and tracking treatment and disease cost with more interest than ever. According to this article on CNN's website, long term care pricing will likely be double by the year 2040. Federal efforts to track and understand how to manage these costs have been increasing and in 2011 President Obama signed into law the National Alzheimer's Project Act , which aims in part to track the costs of dementia for government and society.
What does that mean for us now?
1. If you are an aging person, or advocate for one, consider your current resources and schedule an appointment with an accountant and attorney: they can best advise you of financial expenditures and planning going forward.
2. If you are a Boomer, consider financial/insurance products that insure your dollars will go to best use: whether in an asset-based long-term care policy or financial accounts that have flexibility and guaranteed returns. Because we are living longer, it is imperative to consult a financial advisor to help plan for a comfortable future.
3. If you are an elder advocate, consider meeting with a skilled Care Director like ElderCompass to prioritize and align a plan that makes sense for your particular circumstances.
Don't wait until there is a crisis! Planning ahead is imperative to get positive results and insure wellness and financial peace of mind as you experience the changes aging brings.


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Recording of July 2000: Sonny Rollins: The Freelance Years
Sonny Rollins, tenor sax; Clark Terry, Kenny Dorham, trumpet; Jimmy Cleveland, trombone; Ernie Henry, alto sax; Thelonious Monk, Hank Jones, Sonny Clark, Wynton Kelly, Gil Coggins, Hampton Hawes, piano; Victor Feldman, vibes; Barney Kessel, Oscar Pettiford, Ray Brown, Paul Chambers, Wendell Marshall, Leroy Vinegar, bass; Max Roach, Shelly Manne, Roy Haynes, Kenny Dennis, drums; Abbey Lincoln, vocals
Riverside 5RCD-4427-2 (5 CDs). 2000. Orrin Keepnews, Lester Koenig, Leonard Feather, original prods.; Eric Miller, compilation prod.; Dave Luke, tape transfers; Kirk Felton, remastering. AAD. TT: 5:58:42
Performance *****
Sonics *****
Would that we could reclaim the glory days of hard bop, when giants walked the Earth, without returning to the nadir of legalized apartheid. Would that we were all young again in that blink of an eye before the coming of Ornette Coleman and the ascension of John Coltrane, when Sonny Rollins first hit full stride as a leader and improviser. People still yearn for those bygone days in pre-Beatles America when the second wave of boppers—a remarkable assortment of innovative African-American instrumentalists and composers—sang their freedom song at the tail end of the Jim Crow era.
The Freelance Years commemorates Rollins' arrival as a mature improviser, and complementing the glory of the music is the pristine quality of these new remasterings of the original tapes. For instance, they've reclaimed the lost Monk intro edited on to the front end of "Ba-Lue Bolivar Ba-Lues-Are" on the earlier Monk box (Complete Riverside Recordings, Riverside 22), but minus the noise and crackling.(Perhaps they found a piece of the master tape to replace a scratchy old LP transfer.)
And several remasterings down the road, sweet Jesus, we finally have a definitive rendering of the 1957 Way Out West trio session: newly revealed low-level information and reverb trails surround Rollins' horn, there are greater extension and focus to Ray Brown's bass, and a wealth of newfound details and realistic dynamics in Shelly Manne's drumming—minus the brutal digital sibilance and glare that has marred past transfers. And the enhanced sense of timbral smoothness and soundstage depth to these original masters allows you to delve even deeper into the heart of this pianoless trio's special brand of interplay, and the giddy joy of Rollins' mix of Saturday-afternoon C&W and after-midnight modern jazz. Particular pleasures are Sonny's masterful rhythmic displacements in working his way back into the head of "Way Out West" and his protean effusions on "Come, Gone," the latter inspiring Brown and Manne to swing so hard you could plotz already.
Rollins displays still greater mastery of the trio form a year later with Max Roach and Oscar Pettiford on The Freedom Suite, where his collaborators display an even more advanced feel for spontaneous orchestrations. Rollins makes one of his most profound conceptual statements in the title suite, a heady mix of blues moods and protest. Again, the transparency of these masters allows one to hear more clearly where edits of transitional themes were plugged into the spontaneously conceived sections. Takes in the context of these seminal sessions—Sonny's underrated quartet with Sonny Clarke, Percy Heath, and Roy Haynes, and his west coast super-session with Manne, Leroy Vinegar, Hampton Hawes, and Barney Kessel—shine all the more brightly, particularly on Sonny's cheeky, bravura readings of chestnuts from the Al Jolson canon, "Toot, Toot Tootsie" and "Rock-A-Bye Your Baby With a Dixie Melody."
Elsewhere, Rollins' final appearances as a sideman—with Thelonious Monk, Kenny Dorham, and vocalist Abbey Lincoln—are further indications of his growing mastery. It's wonderful to hear how the saxophonist's terse melodic ideas and intervallic leaps echo and extend Monk's conception on their hilarious romp through the title track, or how he sustains the magisterial elegance of the theme to "Pannonica" through a complex weave of phrases. On the equestrian tempos of "I'll Remember April," he and Roach re-create the galloping dynamism of Brown & Roach, Inc. at their best with erstwhile sub Kenny Dorham. And Rollins' restraint, è la Lester Young, on the Lincoln session adds theatrical drama and melodic tenderness to the singer's tough-love readings of dark ballads, particularly those from the Billie Holiday songbook.
In short, The Freelance Years is a coherent, definitive snapshot of the artist as a young man, just before he left for his sabbatical on the Williamsburg Bridge, where the tenor saxophonist re-invented himself in the first of several creative reassessments that continue apace to this day.—Chip Stern


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Tabs too difficult? Try these video lessons and learn fast
Just play power chords.
Bb Eb Bb Eb
then the little fill is the notes Ab Bb Bb Ab
Bb Eb
Growing sleepy as the rain falls
Bb Eb
As children draped in flowers form a chain
Bb Eb
They sing a song with jelly jars and bird calls
Bb Eb
As night falls into dust and it's day again
Bb Eb
I'm not afraid of a love parade in my daydream
Bb Eb
Old men with kazoos and beating drums
Bb Eb
But I awake and I see the streets are ice cream
Bb Eb Bb
It's just you and me and oh dear, our life has just begun
Eb Bb
Everything is beautiful here
Eb Bb
It's spinning circles around my ears
Eb Bb
I'm finally breaking free from fear
And it's fading
The whole song goes just like that.
Submit corrections
Hard to play? Try these video lessons and learn fast


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Updated from 2:59 p.m. ET with market close information. NEW YORK ( TheStreet) -- State Street ( STT) was the winner among major U.S. financial names on Wednesday, with shares rising 1.5% to close at $69.67. The broad ended mixed, pulling back from earlier gains that followed the release of the Federal Open Market Committee's statement on Federal Reserve monetary policy, which followed the conclusion of a two-day committee meeting. After initial euphoria over the FOMC's decision to make no changes in policy, investors seemed spooked at a small change in language from the previous statement: "The Committee recognizes that inflation persistently below its 2 percent objective could pose risks to economic performance, but it anticipates that inflation will move back toward its objective over the medium term." The main features of the Fed's policy to spur U.S. economic growth have been keeping the short-term federal funds rate in a range of zero to 0.25% since late 2008, and the central bank's long-term bond purchases, which have totaled $85 billion per month since September. The FOMC has said repeatedly, and again on Wednesday, that keeping the federal funds rate in the current "exceptionally low range" would be likely be appropriate at least as long as the U.S. unemployment rate remained above 6.5%. The June unemployment rate was 7.6% and the Labor Department will announce the July unemployment rate on Friday. But investors have been anticipating a tapering of the Fed's bond-buying, sending the market yield for 10-year U.S. Treasury bonds to 2.58% Wednesday afternoon from 1.70% at the end of April. Comments from Federal Reserve chairman Ben Bernanke this month seemed to indicate the Fed might curtail bond purchases as early as September. The 10-year yield was as high as 2.609% earlier on Wednesday. Despite investors apparent discomfort among the wording on inflation, the FOMC on Tuesday made no changes in policy, keeping the federal funds rate in its current range, and continuing the same level of bond-buying, while "maintaining its existing policy of reinvesting principal payments from its holdings of agency debt and agency mortgage-backed securities in agency mortgage-backed securities and of rolling over maturing Treasury securities at auction." This means the Federal Reserve's balance sheet will continue to expand, by a net $85 billion per month, from the securities purchases. UBS economist Maury Harris early on Wednesday in a note to clients predicted that there would be "little change" in the FOMC statement, after members were "likely debating communications strategies" for eventual policy changes. "We still expect the Fed to announce at the September FOMC meeting that they will begin tapering the size of their monthly securities acquisitions," Harris wrote.
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Mary Bailey
Community Editor
The Lilleskogen Park Restoration Plan was amended at the March 18 Scandia City Council meeting to reduce the butterfly garden from 5,000 to a more manageable 2,000 square feet.
Park and Recreation Committee Chair Greg Zauner said the change is temporary; more area can be added later.
The council also voted to have the butterfly garden certified as a Monarch Waystation.
Every fall, monarchs migrate from the U.S. to Mexico, returning in the spring. Milkweeds fuel their flight and feed their caterpillars. Lack of milkweed has contributed to the record low monarch count.
Once common, milkweeds have suffered as farmers switched to genetically modified glyphosate-resistant crops. Milkweeds can survive tilling but not repeated herbicide use.
Land development, weed control in lawns, ditch mowing, drought and temperature extremes are also factors.
To counteract this trend, landowners can plant milkweeds to create a corridor of monarch feeding stations.
Monarch Watch, a nonprofit program, provides seed kits with common milkweed (Asclepias syriaca), swamp milkweed (Asclepias incarnata) and butterfly weed (Asclepias tuberosa).
The city will receive an official sign explaining the site's purpose.
Butterfly garden advocate Janie O'Connor has been presenting monarch seminars in Scandia, recruiting volunteers and donations. At the February Park and Recreation committee meeting, she reported that 11 people have volunteered time, and the Friends of Scandia Parks and Trails and the Scandia-Marine Lions have offered financial support.
The butterfly garden is expected to cost about $4,400. Planting may begin this fall.
After the meeting, O'Connor said the reduction makes sense.
"5,000 square feet is a very large space," she said. "When we looked at all the resources needed (seeds, plugs, water, volunteers, maintenance), it was logical to start with a smaller plot of ground."
Groundwater use
The council voted unanimously to approve a 10-year intergovernmental agreement with other Washington County cities to address groundwater issues. The purpose is to build a coalition; no new regulations or costs are required.
A state law passed in 2012 gives the Department of Natural Resources commissioner authority to create groundwater management areas and limit water use. This could affect residents and businesses who receive water permits.
In Scandia there is no municipal water service. Many residents have private wells, and two water companies, Hilltop and Scandia Water Company, have wells that supply water to downtown businesses and residents.
Temporary dwellings
To make sure the city allows enough time for building high-quality homes, the council amended city code to allow someone building a new home to have a temporary dwelling unit for a year instead of six months.
When the year is up, temporary housing must be removed within 30 days. The amended code will let the city grant an extension allowing up to 90 days for good reason, for example, when parts of the old home are being reused in the new home. Both changes were recommended by the Planning Commission.
Recycling contract
The recycling contract with SRC Inc. of Wyoming was extended for another year. SRC provides homeowners with 65-gallon wheeled carts and picks up recyclable materials every other week. The 2014 cost will be the same as it has been since 2001, $27,846. About 25 percent of this expense is offset by grant revenue from Washington County.
There will be no Scandia Heavy Metal Day this spring. Instead of bringing spring cleanup materials to the community center, residents are invited to take them to SRC at 6320 East Viking Blvd. in Wyoming from April 26 to May 3. Hours are 8 a.m. to 4:30 p.m. Monday through Friday and 8 a.m. to 1:30 p.m. Saturdays. Residents must prove they live in Scandia to receive a 20 percent discount on fees.
Other actions
The council also took the following actions:
– Approved a minor subdivision to create two lots from a 10-acre parcel on Meadowbrook Avenue.
– Accepted a $1,000 donation for Leonard Wojtowicz Skating Park from Roberts Family Funeral Home, organizer of the Vinterfest Scanley Cup hockey tournament, and a $100 donation from the St. Croix Horse and Carriage Society, which holds meetings in the community center.
– Approved a resolution supporting legislation that would allow cities to designate a city website, instead of a local newspaper, to publish public notices. Scandia spends about $2,000 a year on legal notices.
– Renewed an agreement with Girl Scouts of Minnesota and Wisconsin River Valleys for summer 2014 recreation programs at Camp Lakamaga on Big Marine Lake. The city promotes the classes and River Valleys staffs them. Scandia receives 20 percent of the registration fees paid for camps such as archery, skateboarding and tennis; last year the city's portion was $1,121.
– Voted to send City Administrator Kristina Handt to the League of Minnesota Cities annual conference in St. Cloud in June, where she can choose from sessions such as "Tax Increment Financing Oversight" and "City Officials – They Can Be Oh-So-Good and Oh-So-Bad." The registration fee is $325. Handt will stay with relatives, avoiding a hotel charge.
– Heard from Washington County Sheriff's Deputy Chris Majeski of a burglary at Am-Tec Designs, a vehicle theft on Pilar Road and a burglary at an ice house on Big Marine Lake.


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Police Men's 14139SR-04 Sonoran Watch
- Classically styled designer watch from Police
- Scratch resistant hard mineral crystal lens
- Genuine brown leather strap with buckle
- Water resistant up to approximately 30 metres
- In presentation box with 2 year warranty
More product info
Never be late for a meeting or date again with this classically styled watch.
Coming fresh from the designers at Police, this Sonoran watch has a stylish IP rose gold case with a genuine brown leather strap, making it suitable for any occasion. The face features a silver sunray dial with the added bonus of a date feature and is water resistant to 30 metres. The protective scratch resistant, hard mineral crystal lens will keep your watch looking shiny and new for longer and the hypoallergenic stainless steel case makes it perfect even for those with sensitive skin.
Men's Police Sonoran 14139SR-04 Watch – Genuine Leather Strap & Rose Gold Case
Product Specification
- Hypoallergenic stainless steel case
- Scratch resistant hard mineral crystal lens
- Date feature
- Silver sunray dial
- Water resistant to 30 metres
- IP rose gold case
- Genuine brown leather strap
- Quartz movement
- Stainless steel bezel
- Buckle fastening
- Analogue display
- Case diameter 42mm
- Case thickness 8mm
- Band width 22mm
- Weight 5 grams
- 2 year warranty
- Model number 14139JSR/04


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HARRISBURG -- At East Stroudsburg School District, business manager Patricia Bader oversees a reserve fund balance -- one of the largest among the 500 school districts in Pennsylvania.
But the district's more than $34 million fund balance will not be tapped to help close an expected $10 million shortfall in a proposed $143 million budget.
Instead, residents of the district -- which straddles the line between Monroe and Pike counties in the state's northeast corner -- face a possible 2.3 percent property tax hike, the highest allowed for school districts under state law. In addition, several vacant positions will not be filled.
Bader said the school board was unwilling to dip into the reserves to close the budget hole.
"We didn't want to go into that, because the future looks as bleak as this year," Bader said.
She was referring to growing pension costs for public school employees the district plans to cover with the reserves in coming years.
Districts must pay for half of the pension costs -- with the state filling in the rest -- which are forecast to nearly triple in the next four years and remain at that height for decades to come.
Gov. Tom Corbett's administration, meanwhile, is encouraging districts with large reserve funds -- like East Stroudsburg -- to dip into those accounts to cover operating expenses in a year when state education funding is increasing, but not as much as districts would like.
CLICK HERE for a complete listing of school districts and what they have in reserve.
"School districts that are claiming that they are losing money, many of them should look into their fund balances and using those accounts to help fund academic programs," Tim Eller, spokesman for the state Department of Education, said.
Eller said keeping a small amount of money in an emergency reserve fund is prudent, but many districts have stockpiled cash that could be used to close budget holes.
As of June 30 last year, school districts statewide were sitting on more than $3.2 billion above what was needed to pay expenses for the 2010-11 fiscal year, according to Department of Education statistics.
Pittsburgh School District leads districts statewide with more than $168 million in reserve, but nine other districts have at least $25 million tucked away (see chart).
However, 13 districts have reserve funds that are overdrawn and are in the negative -- led by Philadelphia City School District, with a negative balance of $118 million.
Another 13 districts have reserve funds with less than $500,000, mostly consisting of small or poor districts.
The median average for the state -- the point at which 250 districts have larger funds and 250 districts have smaller funds -- is about $5.1 million in budgetary reserves.
Facing another round of tough budget choices, some districts are tapping their reserve funds.
The school board in Downingtown Area School District in Chester County -- which holds more than $50 million in reserve, the second largest total in the state -- is doing just that.
Patricia McGlone, the district's chief financial officer, said the decision to pull $10 million out of the savings account was not made easily, but reflects the difficulty of this year's budget while maintaining some savings for future construction and pension costs.
"I think we're respecting the decision of past school boards to put this money away for future generations, and we're using it prudently," she said.
The district's preliminary budget for next year also includes a 1.96 percent property tax increase that McGlone said could shrink to 1.7 percent if state aid increases.
Oxford Area School District in Chester County -- which has a reserve fund of $17 million, well above the state average -- also plans to raise taxes by 1.7 percent and will drain about $2 million to close a shortfall in the district's $57 million budget.
Financial industry standards suggest that pulling more than 10 percent out of savings in a single year is not prudent.
The Pennsylvania School Boards Association's "advice to districts is that reserve fund revenue is one-time revenue, so if you're going to use it, use for one-time expenditures," said Dave Devare, director of research services for PSBA.
About 70 percent of the state's 500 districts are tapping into their reserve funds in their preliminary budgets, according to a survey by the Pennsylvania Association of School Business Officers, a professional association that helps provide products and services for school business management.
Jay Himes, executive director of PASBO, said the administration is taking a different view on using reserve funds after being critical of districts that built federal stimulus dollars -- another one-time revenue source -- into their budgets in recent years, creating a funding cliff.
"This is time to use it, but you have to be careful and selective in how you use it," Himes said of the reserve funds.
Districts also can classify their reserve funds differently.
Committed and assigned funds have been earmarked for one purpose but have not been used. About $1.7 billion -- a little more than half -- of the total reserve funds districtwide fall into those categories.
The remaining $1.5 billion are unassigned reserve funds, which can be used for anything from filling budget holes to paying pension costs and constructing new facilities.
State law prevents districts from raising taxes if they have more than 8 percent of their overall budget in unassigned reserves, so districts will store money in the other types of funds to get around that restriction.
Since the committed and assigned funds have not been used, they can be repurposed by a simple vote of the local school board, Eller said.
District Reserve Funds:�The Top 10
Downingtown -�$50 million
Abington - $45 million
Lower Merion - $43 million
Altoona - $37 million
Reading -�$36 million
East Stroudsburg -�$34 million
Tredyffrin-Easttown -�$31 million
Bensalem -�$28 million
Council Rock -�$27 million
State Total


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Financial Aid High School Counselors Page
Midland College Financial Aid forms are available for download in Adobe PDF format. To read and print these forms you will need Adobe Acrobat Reader.
All documents must be submitted by a High School Counselor.
• MC High School Counselor's Recommendation Scholarship
• Fasken Foundation Top 5% Scholarship


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From OpenWetWare
ATTO Dyes
We use the Atto dyes Atto 550 and Atto 647N.
DAPI is an unfused aromatic ring system linked by bonds with torsional freedom which allow the molecules to adopt appropriate conformation to fit the helical curvature of the groove without significant perturbation of the DNA. DAPI has demonstrated DNA binding via intercalation with poly[d(GC)]. It is a nonclasical intercalator. (Strekowski et.al.)
DNA Binders
Lots of small molecules bind to DNA, with highly diverse effects. Nevertheless, most compounds can be classified according to their binding mode: major groove binder, minor groove binder, or intercalator. The last ones insert between neighbored bases. Here we examined one example for each of these groups:
|Binding mode
|minor groove binding
|0.9 nM
|8.3 µM
|major groove binding
|4.8 µM
|Ethidium bromide
|12.0 µM
Ethidium BromideEthidium bromide is a classical intercalator. It is a fused-ring aromatic molecule with positive charges on an attached side chain and/or the ring system itself. The planar ring system stacks well with base pairs in the intercalation. Ethidium bromide intercalates from the minor groove of the duplex complex. (Strekowski et.al.)
FOBFOB is short for folding buffer. It contains 50mM Tris and 10mM EDTA. FOBxM refers to FOB without MgCl2, whereas FOB20 is FOB with 20mM MgCl2
In short, FRET is a fluorescence based approach often used in biosciences to measure distances in the lengthscale of up to ca. 10nm. For more explanation, please read the entry in the methods section.
IntercalationIntercalation is known to occur without interfering with hydrogen bonding of the base pairs and obeys the nearest neighbor exclusion principle. When DNA is saturated with intercalators, every second potential intercalation site on the helix remains empty. In order for intercalation to ensue, theDNAbase pairs separate 3.4 °A to form a cavity for the incoming planar ligand through localized left-handed unwinding of the duplex. The normal twist of B-formDNA is 36◦ (10 base pairs per one turn of 360◦). In order to accommodate the ligand, a reduction of this rotation occurs. The unwinding angle varies with the geometry of the ligand–DNA complex.
In spite of the wealth of structural data known for intercalation complexes, the origin of the intercalation forces is not fully understood. Intercalation has been generally considered to be the result of a hydrophobic interaction in which a hydrophobic aromatic molecule is drawn to a hydrophobic environment of the base pairs from the hydrophilic aqueous environment.
SpermineSpermine is a polyamine, which is protonated under physiological conditions and therefore interacts strongly with DNA. All theoretical and experimental results (see: Noncovalent interactions with DNA: An overview) can be rationalized in terms of the concept of the negative potentials in the DNA macromolecule. It has been concluded that the highest concentration of the negative potentials is in the grooves of the DNA duplexes rather than on the backbones, despite the location of the anionic phosphates within these backbones. The negative potentials are stronger in the major groove than in the minor groove at GC sequences. Accordingly, a flexible cationic molecule of protonatedspermine forms a complex within the DNA major groove with GC sequences following the negative potential concentration. (Strekowski et.al.)
Transmission Electron Microscopy (TEM)Transmission electron microscopy is an imaging technique to observe thin specimen. An electron beam interacts with the specimen while passing and is detected by a sensor.
Organic samples can be prepared with high atomic number stains to enhance contrast. Electrons can pass through the biological structure and are absorbed or scattered by the stain.